WorldWideScience

Sample records for bloodless rapid transection

  1. Rapid transdermal bloodless and reagent-free malaria detection

    Science.gov (United States)

    Lukianova-Hleb, Ekaterina Y.; Campbell, Kelly M.; Constantinou, Pamela E.; Braam, Janet; Olson, John S.; Ware, Russell E.; Sullivan, David S.; Lapotko, Dmitri

    2014-02-01

    Successful diagnosis, screening, and elimination of malaria critically depend on rapid and sensitive detection of this dangerous infection, preferably transdermally and without sophisticated reagents or blood drawing. Such diagnostic methods are not currently available. Here we show that the high optical absorbance and nanosize of endogenous heme nanoparticles called hemozoin, a unique component of all blood-stage malaria parasites, generate a transient vapor nanobubble around hemozoin in response to a short and safe near-infrared picosecond laser pulse. The acoustic signals of these malaria-specific nanobubbles provided the first transdermal non-invasive and rapid detection of a malaria infection as low as 0.00034% in animals without using any reagents or drawing blood. These on-demand transient events have no analogs among current malaria markers and probes, can detect and screen malaria in seconds and can be realized as a compact, easy to use, inexpensive and safe field technology.

  2. Bloodless, sutureless circumcision

    Directory of Open Access Journals (Sweden)

    Nisar A Bhat

    2013-01-01

    Full Text Available Background: W e present our experience of sutureless and bloodless elective circumcision in neonates and infants with Gomco clamp. Patients and Methods: From March 2008 to May 2011, 200 babies with age ranging from 2 weeks to 7 months underwent Gomco circumcision. All patients were given chlorohydrate 50 mg/kg, paracetamol suppository 15 mg/kg, and local anesthesia. Procedure was done in minor operation theatre (OT and babies were observed for 1 h in recovery room before discharging them home. Results: Two of our patients (1% required immediate suturing on table after Gomco clamp was removed, five patients (2.5% were shifted back from the recovery room to minor OT for suture repair and eight patients (4% required reinforcement of primary dressing to control the minor ooze. There was no other complication. Cosmesis was to the satisfaction of the surgeon as well as the parents. Conclusion: Gomco clamp is a bloodless, sutureless, simple, and safe method of circumcision in newborns and infants. It is cost-effective and can be performed under local anesthesia and sedation with excellent cosmetic results.

  3. Current role of bloodless liver resection

    Institute of Scientific and Technical Information of China (English)

    Spiros G Delis; Juan Madariaga; A Bakoyiannis; Ch Dervenis

    2007-01-01

    Liver resections are demanding operations which can have life threatening complications although they are performed by experienced liver surgeons. Recently new technologies are applied in the field of liver surgery, having one goal: safer and easier liver operations. The aim of this article is to address the issue of bloodless liver resection using radiofrequency energy. Radionics, Cool-tipTM System and Tissue Link are some of the devices which are using radiofrequency energy. All information included in this article, refers to these devices in which we have personal experience in our unit of liver surgery. These devices take advantage of its unique combination of radiofrequency current and internal electrode cooling to perform sealing of the small vessels and biliary radicals. Dissection is also feasible with the cool-tip probe. For the purposes of this study patient sex, age, type of disease and type of surgical procedure in association with the duration of parenchymal transection, blood loss, length of hospital stay, morbidity and mortality were analyzed. Cool-tip RF device may provide a unique, simple and rather safe method of bloodless liver resections if used properly. It is indicated mostly in cirrhotic patients with challenging hepatectomies (segment W, central resections). The total operative time is eliminated and the average blood loss is significantly decreased. It is important to note that this technique should not be applied near the hilum or the vena cava to avoid damage of these structures.

  4. Benthic data from rapid assessment transects, 2001-2004, in the Hawaiian Islands (NODC Accession 0002464)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This dataset consists of CRAMP Rapid Assessment Transect surveys taken in 2001-2004 and includes quantitative estimates of substrate type and species. The types and...

  5. Substrate and species data from Coral Reef Assessment and Monitoring Program (CRAMP) Rapid Assessment Transect surveys from 2006 in the coastal waters of Hawaii, 22 June - 12 December 2006 (NODC Accession 0039383)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This dataset consists of CRAMP Rapid Assessment Transect surveys taken in 2006 and includes quantitative estimates of substrate type and species. In 2006, there...

  6. Lake Transect : 1986

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — This document summarizes transect surveys that were done at four different lakes on St. Vincent National Wildlife Refuge in 1986. Lists of the plant species found...

  7. Lake Transect : 1988

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — This document summarizes transect surveys that were done at four different lakes on St. Vincent National Wildlife Refuge in 1988. Lists of the plant species found...

  8. Lake Transect : 1987

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — This document summarizes transect surveys that were done at four different lakes on St. Vincent National Wildlife Refuge in 1987. Lists of the plant species found...

  9. Lake Transect : 1990

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — This document summarizes transect surveys that were done at four different lakes on St. Vincent National Wildlife Refuge in 1990. Lists of the plant species found...

  10. Lake Transect : 1989

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — This document summarizes transect surveys that were done at four different lakes on St. Vincent National Wildlife Refuge in 1989. Lists of the plant species found...

  11. CRED REA Fish Team Belt Transect Survey at Kauai, Main Hawaiian Islands, 2006

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Belt transects along 3 consecutively-placed, 25m transect lines were surveyed as part of Rapid Ecological Assessments conducted at 7 sites at Kauai in the Main...

  12. CRED REA Fish Team Belt Transect Survey at Ofu-Olosega, American Samoa, 2006

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Belt transects along 3 consecutively-placed, 25m transect lines were surveyed as part of Rapid Ecological Assessments conducted at 12 sites at Ofu-Olosega in the...

  13. CRED REA Fish Team Belt Transect Surveys at Maro Reef, 2002

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Belt transects along 3 consecutively-placed, 25m transect lines were surveyed as part of Rapid Ecological Assessments conducted at 5 sites at Maro Reef in October,...

  14. CRED REA Fish Team Belt Transect Surveys at Pearl and Hermes Reef, 2002

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Belt transects along 3 consecutively-placed, 25m transect lines were surveyed as part of Rapid Ecological Assessments conducted at 17 sites at Pearl Hermes Reef in...

  15. CRED REA Fish Team Belt Transect Surveys at Laysan Island, 2002

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Belt transects along 3 consecutively-placed, 25m transect lines were surveyed as part of Rapid Ecological Assessments conducted at 3 sites at Laysan Island in...

  16. CRED REA Fish Team Belt Transect Surveys at French frigate Shoals, 2002

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Belt transects along 3 consecutively-placed, 25m transect lines were surveyed as part of Rapid Ecological Assessments conducted at 12 sites at French Frigate Shoals...

  17. CRED REA Fish Team Belt Transect Surveys at the Midway Atoll, 2002

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Belt transects along 3 consecutively-placed, 25m transect lines were surveyed as part of Rapid Ecological Assessments conducted at 12 sites at Midway Atoll in...

  18. CRED REA Fish Team Belt Transect Surveys at Johnston Island, 2004

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Belt transects along 3 consecutively-placed, 25m transect lines were surveyed as part of Rapid Ecological Assessments conducted at 12 sites at Johnston Island in...

  19. CRED REA Fish Team Belt Transect Survey at Santa Rosa Bank, Marianas Archipelago, 2005

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Belt transects along 3 consecutively-placed, 25m transect lines were surveyed as part of Rapid Ecological Assessments conducted at 2 sites at Santa Rosa Bank in the...

  20. CRED REA Fish Team Belt Transect Survey at Santa Rosa Reef, 2003

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Belt transects along 3 consecutively-placed, 25m transect lines were surveyed as part of Rapid Ecological Assessments conducted at 2 sites at Santa Rosa Reef in the...

  1. CRED REA Fish Team Belt Transect Surveys at Midway Atoll, 2003

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Belt transects along 3 consecutively-placed, 25m transect lines were surveyed as part of Rapid Ecological Assessments conducted at 9 sites at Midway Atoll in July,...

  2. CRED REA Fish Team Belt Transect Survey at Rose, American Samoa, 2006

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Belt transects along 3 consecutively-placed, 25m transect lines were surveyed as part of Rapid Ecological Assessments conducted at 12 sites at Rose in the American...

  3. CRED REA Fish Team Belt Transect Survey at Midway, NW Hawaiian Islands, 2006

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Belt transects along 3 consecutively-placed, 25m transect lines were surveyed as part of Rapid Ecological Assessments conducted at 9 sites at Midway in the NW...

  4. CRED REA Fish Team Belt Transect Survey at Pathfinder Reef, Marianas Archipelago, 2005

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Belt transects along 3 consecutively-placed, 25m transect lines were surveyed as part of Rapid Ecological Assessments conducted at 2 sites at Pathfinder Reef in the...

  5. CRED REA Fish Team Belt Transect Survey at Baker, Pacific Remote Island Areas in 2008

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Belt transects along 3 consecutively-placed, 25m transect lines were surveyed as part of Rapid Ecological Assessments conducted at 4 sites at Baker in the Pacific...

  6. CRED REA Fish Team Belt Transect Survey at Ta'u, 2004

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Belt transects along 3 consecutively-placed, 25m transect lines were surveyed as part of Rapid Ecological Assessments conducted at 9 sites at Ta'u Islands in...

  7. Consequences of Neurite Transection In Vitro

    Science.gov (United States)

    Cengiz, Nurettin; Erdoğan, Ender; Him, Aydın; Oğuz, Elif Kaval

    2012-01-01

    Abstract In order to quantify degenerative and regenerative changes and analyze the contribution of multiple factors to the outcome after neurite transection, we cultured adult mouse dorsal root ganglion neurons, and with a precise laser beam, we transected the nerve fibers they extended. Cell preparations were continuously visualized for 24 h with time-lapse microscopy. More distal cuts caused a more elongated field of degeneration, while thicker neurites degenerated faster than thinner ones. Transected neurites degenerated more if the uncut neurites of the same neuron simultaneously degenerated. If any of these uncut processes regenerated, the transected neurites underwent less degeneration. Regeneration of neurites was limited to distal cuts. Unipolar neurons had shorter regeneration than multipolar ones. Branching slowed the regenerative process, while simultaneous degeneration of uncut neurites increased it. Proximal lesions, small neuronal size, and extensive and rapid neurite degeneration were predictive of death of an injured neuron, which typically displayed necrotic rather than apoptotic form. In conclusion, this in vitro model proved useful in unmasking many new aspects and correlates of mechanically-induced neurite injury. PMID:20121423

  8. The traveling transect

    DEFF Research Database (Denmark)

    Braae, Ellen Marie; Diedrich, Lisa; Lee, Gini

    2013-01-01

    , when working with the more unpredictable qualities of sites as in water-made landscapes, designers often lack mapping and representational tools capable of capturing and expressing ephemeral qualities - dynamics, relationships and atmospheres. These abstract qualities, that exist over physical site...... in everyday practice, the Canarysect project negotiates testing and capture of the dynamic, relational and atmospheric qualities encountered along lines of transect across island lands and waters. Individual sketching, photography and modelling gestures merge into a common archipelago of thinking around...... the water landscapes of the Canaries. Through the medium of the Nordes 2013 exhibition, coexisting tableaux of imagery and form produce another mapping of already-known island landscapes, brought to contemporary presence through a gaze informed by the layered histories of the landscapes and peoples, sites...

  9. Radiofrequency ablation-assisted liver resection:a step toward bloodless liver resection

    Institute of Scientific and Technical Information of China (English)

    Athanasios Petrou; Kyriakos Neofytou; Constantinos Mihas; Jessamy Bagenal; Michael Kontos; John Griniatsos; Evangelos Felekouras

    2015-01-01

    BACKGROUND: Liver resection is currently the most efficient curative approach for a wide variety of liver tumors. The ap-plication of modern techniques and new surgical devices has improved operative outcomes. Radiofrequency ablation is used more often for liver parenchymal transection. This study aimed to assess the efficacy and safety of radiofrequency abla-tion-assisted liver resection. METHODS: A retrospective study of 145 consecutive patients who underwent radiofrequency ablation-assisted liver resec-tion was performed. Intraoperative blood loss, need for trans-fusion or intraoperative Pringle maneuver, the duration of liver parenchymal transection, perioperative complications, and postoperative morbidity and mortality were all evaluated. RESULTS: Fifty minor and ninety-five major liver resections were performed. The mean intraoperative blood loss was 251 mL, with a transfusion rate of 11.7%. The Pringle maneuver was necessary in 12 patients (8.3%). The mean duration for parenchymal transection was 51.75 minutes. There were 47 patients (32.4%) with postoperative complications. There is no mortality within 30 days after surgery. CONCLUSIONS: Radiofrequency ablation-assisted liver re-section permits both major and minor liver resections with minimal blood loss and without occlusion of hepatic inflow. Furthermore it decreases the need for blood transfusion and reduces morbidity and mortality.

  10. CRED REA Fish Team Belt Transect Survey at Palmyra Atoll, Pacific Remote Island Areas (PRIAs) in 2006

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Belt transects along 3 consecutively-placed, 25m transect lines were surveyed as part of Rapid Ecological Assessments conducted at 13 sites at Palmyra Atoll in the...

  11. Use of a low-prime circuit for bloodless heart transplantation in xenotransplant of 5-7 kilogram primates.

    Science.gov (United States)

    Gomez, D; Olshove, V F; Weinstein, S; Davis, J T; Michler, R E

    2000-09-01

    There is a great effort to decrease blood product use during open-heart surgery in pediatrics. We were presented with a research challenge to accomplish heart xenotransplantation from donor cynomologous monkey (Macaca fascicularis) to recipient olive baboon (Papio anubis) of 5-7 kilograms without benefit of donor or banked blood products. The purpose of this study was to design and implement a practical, low-volume circuit to minimize hemodilution and avoid the use of blood products. A simple circuit was assembled using a low-volume oxygenator with hardshell venous reservoir, an 1/8-inch arterial line, an 1/4-inch venous line, and gravity drainage. Three xenotransplants were performed and evaluated. The mean recipient weights were 6.3 +/- 0.7 kg. Circuit prime volume was 228 +/- 5.8 mL, and bypass time was 85 +/- 6.7 min. Blood flow rates were 585 +/- 113 mL/min with postmembrane arterial line pressures of 344 +/- 81 mmHg, and patient mean arterial pressures (MAP) of 51.4 +/- 16.7 mmHg. Venous saturations were 63.7 +/- 8.0%. The hematocrit prebypass was 37.4 +/- 3.2, bypass 20.7 +/- 0.9, post-MUF 27.8 +/- 3.3, and 7 days postoperative 24.5 +/- 7.5%. Platelet count was 289 +/- 1.1 K/microL, 147 +/- 37.1 K/microL, and 322 +/- 292.7 K/microL prebypass, postbypass, and 7 days postoperative, respectively. Plasma-free hemoglobin prebypass was 7.5 +/- 4.4 mg/dL and postbypass 22.2 +/- 16.5 mg/dL with no noted hematuria during and after the procedure. All patients survived and were successfully weaned from cardiopulmonary bypass (CPB) with same day extubation. A low-prime circuit for bloodless heart surgery is possible. To achieve low reservoir levels, especially without the use of an arterial line filter (ALF), it is necessary to have a full armament of monitoring and alarm devices.

  12. James F. McGinnis, D.C., N.D., C.P. (1873-1947): spinographer, educator, marketer and bloodless surgeon.

    Science.gov (United States)

    Keating, J C

    1998-12-01

    Perhaps best remembered for his contributions to B.J. Palmer's earliest developments in spinography, James F. McGinnis also pioneered in marketing methods while a straight chiropractic practitioner in Iowa. His advertising brought him to the attention of organized medicine, which sought his prosecution. Relocating to California in the early 1920s, he broadened his scope of practice and earned a naturopathic doctorate. In the 1930s he became one of the best known of several chiropractic bloodless surgeons and traveled around the nation to teach his methods. Although initially a passionate member of the Universal Chiropractors Association and receptive to Palmer's introduction of the neurocalometer, McGinnis eventually changed his political allegiance and became an active member of the National Chiropractic Association. He died in 1947 while on a teaching tour of Claifornia's San Joaquin Valley. PMID:11623684

  13. Moist soil vegetative transect results - 1985

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — Moist soil impoundment (MSI) construction at Duck River unit was completed in 1985 and water management was initiated. Permanent transects were established within...

  14. Transect 23:2 (autumn 2005)

    OpenAIRE

    UC Natural Reserve System

    2007-01-01

    IN THIS ISSUE OF TRANSECT - (feature article) New Laser-Mapping Technology Enables "Aerial Groundtruthing" - Marine biologist Dan Costa at the TOPP of His Game - Teens Learn Leadership and Literacy in the Sierras at Sagehen Creek Field Station - Kids Star in Bug Video Produced at Valentine Reserve - Sedgwick Reserve Celebrates New All-Green Science Center

  15. Fractured cervical spine and aortic transection.

    LENUS (Irish Health Repository)

    Griffin, M J

    2012-02-03

    A 17-year-old victim of a road traffic accident presented. Following investigation diagnoses of fractured first cervical vertebra, aortic transection, diffuse cerebral oedema, fractured right ribs 2-4 and pubic rami were made. Management of this case presented a number of anaesthetic dilemmas: management of the airway, use of cross-clamp vs. shunting or heparinization and bypass, cardiovascular and neurological monitoring, maintenance of cardiovascular stability during and post cross-clamp, minimizing the risk of post-operative renal and neurological dysfunction.

  16. Likelihood-based inference for clustered line transect data

    DEFF Research Database (Denmark)

    Waagepetersen, Rasmus Plenge; Schweder, Tore

    The uncertainty in estimation of spatial animal density from line transect surveys depends on the degree of spatial clustering in the animal population. To quantify the clustering we model line transect data as independent thinnings of spatial shot-noise Cox processes. Likelihood-based inference...

  17. Likelihood-based inference for clustered line transect data

    DEFF Research Database (Denmark)

    Waagepetersen, Rasmus; Schweder, Tore

    2006-01-01

    The uncertainty in estimation of spatial animal density from line transect surveys depends on the degree of spatial clustering in the animal population. To quantify the clustering we model line transect data as independent thinnings of spatial shot-noise Cox processes. Likelihood-based inference...

  18. Vegetative Transect Results : Clarence Cannon National Wildlife Refuge : 1980

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — This report summarizes the result of 27 vegetative transects conducted on 21 fields of the Clarence Cannon National Wildlife Refuge. The methods used to collect the...

  19. Avian Point Transect Survey; Seward Peninsula, Alaska, 2012

    Data.gov (United States)

    U.S. Geological Survey, Department of the Interior — This data product contains avian point-transect survey data and habitat data collected on the Seward Peninsula, Alaska, USA, during 21 May – 10 June 2012. We...

  20. Open hepatic parenchymal transection using ultrasonic dissection and bipolar coagulation

    OpenAIRE

    Lesurtel, Mickael; Belghiti, Jacques

    2008-01-01

    Liver transection is the most challenging part of liver resection due to the risk of massive blood loss which is associated with increased postoperative morbidity and mortality, as well as reduced long-term survival after resection of malignancies. Among the devices used for open parenchyma transection, ultrasonic dissection with bipolar cautery forceps is one of the most widely used technique worldwide. We identified four retrospective comparative studies and three randomized controlled tria...

  1. Isolated Pediatric Pancreatic Transection Secondary to Ocean-Related Trauma

    Directory of Open Access Journals (Sweden)

    Afif N Kulaylat

    2013-03-01

    Full Text Available Context Isolated pancreatic transection is a rare but well-recognized complication following blunt trauma of the abdomen. Diagnosis at presentation may be difficult and delayed due to subtle initial symptoms and evolving nature of the injury. Case report We describe an isolated complete pancreatic transection in a 14-year-old female secondary to a previously unreported and highly unusual mechanism (being tossed by a wave. Diagnosis was obtained by computed tomography scan 24 hours following initial trauma. She was managed operatively with an open distal pancreatectomy with splenic preservation and no subsequent complications. Conclusions The force sustained from the blunt abdominal trauma of being tossed by a wave can be significant. The management of pancreatic injuries in children, particularly in the context of ductal transection, is controversial. Timely recognition and management is critical to optimal outcomes. Early operative intervention may help to avoid complications such as abscess or pseudocyst formation.

  2. Persistent bilateral function after unilateral facial nerve transection

    Directory of Open Access Journals (Sweden)

    A Sehgal

    2014-01-01

    Full Text Available To indicate that occasionally the contralateral facial nerve provides redundant innervation to the hemi-face. Single case report. We report the case of a patient whose facial nerve was transected during removal of a 3 cm vestibular schwannoma. Postoperatively, this patient still demonstrated spontaneous mimetic motion on the side of the face ipsilateral to the transsection. Review of the literature finds rare instances of preserved function after nerve transection in the removal of large parotid masses. We speculate that the slow loss of ipsilateral function can at times contribute to innervation from the contralateral facial nerve.

  3. Edge effect on carabid assemblages along forest-grass transects

    OpenAIRE

    Magura, T.; B. Tóthmérész; T. Molnár

    2001-01-01

    During 1997 and 1998, we have tested the edge-effect for carabids along oak-hornbeam forest-grass transects using pitfall traps in Hungary. Our hypothesis was that the diversity of carabids will be higher in the forest edge than in the forest interior. We also focused on the characteristic species of the habitats along the transects and the relationships between their distribution and the biotic and abiotic factors.

    Our results proved that there was a significant edge eff...

  4. Open hepatic parenchymal transection using ultrasonic dissection and bipolar coagulation.

    Science.gov (United States)

    Lesurtel, Mickael; Belghiti, Jacques

    2008-01-01

    Liver transection is the most challenging part of liver resection due to the risk of massive blood loss which is associated with increased postoperative morbidity and mortality, as well as reduced long-term survival after resection of malignancies. Among the devices used for open parenchyma transection, ultrasonic dissection with bipolar cautery forceps is one of the most widely used technique worldwide. We identified four retrospective comparative studies and three randomized controlled trials dealing with the efficacy of ultrasonic dissector (UD) compared with other techniques including the historical clamp crushing technique. UD is associated with similar blood loss and slower resection time compared with water-jet or clamp crushing technique. However, it seems to be more precise in dissecting vessels. Its use does not impact on morbidity and hospital stay compared with other techniques. From an economic point of view, UD is the most expensive technique and may be a disadvantage for low centre volume. UD with bipolar cautery is one of the safest and the most efficient device for liver transection, even if its superiority over the clamp crushing technique has not been well established. It is considered as a standard technique for liver transection. PMID:18773097

  5. Edge effect on carabid assemblages along forest-grass transects

    Directory of Open Access Journals (Sweden)

    T. Magura

    2001-02-01

    Full Text Available During 1997 and 1998, we have tested the edge-effect for carabids along oak-hornbeam forest-grass transects using pitfall traps in Hungary. Our hypothesis was that the diversity of carabids will be higher in the forest edge than in the forest interior. We also focused on the characteristic species of the habitats along the transects and the relationships between their distribution and the biotic and abiotic factors.

    Our results proved that there was a significant edge effect on the studied carabid communities: the Shannon diversity increased significantly along the transects from the forest towards the grass. The diversity of the carabids were significantly higher in the forest edge and in the grass than in the forest interior. The carabids of the forest, the forest edge and the grass are separated from each other by principal coordinates analysis and by indicator species analysis (IndVal, suggesting that each of the three habitats has a distinct species assemblages. There were 5 distinctive groups of carabids: 1 habitat generalists, 2 forest generalists, 3 species of the open area, 4 forest edge species, and 5 forest specialists. It was demonstrated by multiple regression analyses, that the relative air moisture, temperature of the ground, the cover of leaf litter, herbs, shrubs and canopy cover, abundance of the carabids’ preys are the most important factors determining the diversity and spatial pattern of carabids along the studied transects.

  6. Transect 16:1 (spring/summer 1998)

    OpenAIRE

    UC Natural Reserve System

    1998-01-01

    IN THIS ISSUE OF TRANSECT - (feature article) Science, Art, and Community Service Converge When Kids Meet in the Carpinteria Marsh "Classroom" - Carpinteria Marsh Kids - Multilevel Outreach at SNARL/Valentine Camp - Jepson Prairie Docents - Wilderness Medicine at James San Jacinto Mtns - Many, Many Thanks to the Packard Foundation!

  7. A hierarchical modeling framework for multiple observer transect surveys.

    Directory of Open Access Journals (Sweden)

    Paul B Conn

    Full Text Available Ecologists often use multiple observer transect surveys to census animal populations. In addition to animal counts, these surveys produce sequences of detections and non-detections for each observer. When combined with additional data (i.e. covariates such as distance from the transect line, these sequences provide the additional information to estimate absolute abundance when detectability on the transect line is less than one. Although existing analysis approaches for such data have proven extremely useful, they have some limitations. For instance, it is difficult to extrapolate from observed areas to unobserved areas unless a rigorous sampling design is adhered to; it is also difficult to share information across spatial and temporal domains or to accommodate habitat-abundance relationships. In this paper, we introduce a hierarchical modeling framework for multiple observer line transects that removes these limitations. In particular, abundance intensities can be modeled as a function of habitat covariates, making it easier to extrapolate to unsampled areas. Our approach relies on a complete data representation of the state space, where unobserved animals and their covariates are modeled using a reversible jump Markov chain Monte Carlo algorithm. Observer detections are modeled via a bivariate normal distribution on the probit scale, with dependence induced by a distance-dependent correlation parameter. We illustrate performance of our approach with simulated data and on a known population of golf tees. In both cases, we show that our hierarchical modeling approach yields accurate inference about abundance and related parameters. In addition, we obtain accurate inference about population-level covariates (e.g. group size. We recommend that ecologists consider using hierarchical models when analyzing multiple-observer transect data, especially when it is difficult to rigorously follow pre-specified sampling designs. We provide a new R package

  8. A hierarchical modeling framework for multiple observer transect surveys.

    Science.gov (United States)

    Conn, Paul B; Laake, Jeffrey L; Johnson, Devin S

    2012-01-01

    Ecologists often use multiple observer transect surveys to census animal populations. In addition to animal counts, these surveys produce sequences of detections and non-detections for each observer. When combined with additional data (i.e. covariates such as distance from the transect line), these sequences provide the additional information to estimate absolute abundance when detectability on the transect line is less than one. Although existing analysis approaches for such data have proven extremely useful, they have some limitations. For instance, it is difficult to extrapolate from observed areas to unobserved areas unless a rigorous sampling design is adhered to; it is also difficult to share information across spatial and temporal domains or to accommodate habitat-abundance relationships. In this paper, we introduce a hierarchical modeling framework for multiple observer line transects that removes these limitations. In particular, abundance intensities can be modeled as a function of habitat covariates, making it easier to extrapolate to unsampled areas. Our approach relies on a complete data representation of the state space, where unobserved animals and their covariates are modeled using a reversible jump Markov chain Monte Carlo algorithm. Observer detections are modeled via a bivariate normal distribution on the probit scale, with dependence induced by a distance-dependent correlation parameter. We illustrate performance of our approach with simulated data and on a known population of golf tees. In both cases, we show that our hierarchical modeling approach yields accurate inference about abundance and related parameters. In addition, we obtain accurate inference about population-level covariates (e.g. group size). We recommend that ecologists consider using hierarchical models when analyzing multiple-observer transect data, especially when it is difficult to rigorously follow pre-specified sampling designs. We provide a new R package, hierarchical

  9. Breton Island, Louisiana Transects with Shoreline Change Rates (Post Hurricane Katrina) (Geographic, NAD83)

    Data.gov (United States)

    U.S. Geological Survey, Department of the Interior — Breton Island, Louisiana Transects with Shoreline Change Rates (Post Hurricane Katrina) (Geographic, NAD83) consists of vector transect data that was derived from...

  10. Terrestrial transect study on driving mechanism of vegetation changes

    Institute of Scientific and Technical Information of China (English)

    2008-01-01

    In terms of Chinese climate-vegetation model based on the classification of plant functional types, to- gether with climatic data from 1951 to 1980 and two future climatic scenarios (SRES-A2 and SRES-B2) in China from the highest and the lowest emission scenarios of greenhouse gases, the distribution patterns of vegetation types and their changes along the Northeast China Transect (NECT) and the North-South Transect of Eastern China (NSTEC) were simulated in order to understand the driving mechanisms of vegetation changes under climatic change. The results indicated that the vegetation distribution patterns would change significantly under future climate, and the major factors driving the vegetation changes were water and heat. However, the responses of various vegetation types to the changes in water and heat factors were obviously different. The vegetation changes were more sensi- tive to heat factors than to water factors. Thus, in the future climate warming will significantly affect vegetation distribution patterns.

  11. Multiscale entropy-based analyses of soil transect data

    OpenAIRE

    Tarquis Alfonso, Ana Maria; Bird, N. R. A.; Whitmore, A.P.; Cartagena Causape, Maria Carmen; Pachepsky, Yakov

    2008-01-01

    A deeper understanding of the spatial variability of soil properties and the relationships between them is needed to scale up measured soil properties and to model soil processes. The object of this study was to describe the spatial scaling properties of a set of soil physical properties measured on a common 1024-m transect across arable fields at Silsoe in Bedfordshire, east-central England. Properties studied were volumetric water content ({theta}), total porosity ({pi}), pH, and N2O flux. ...

  12. Sleeve resection for delayed presentation of traumatic bronchial transection.

    LENUS (Irish Health Repository)

    Mohamed, H Y

    2010-02-01

    Tracheobronchial disruption is uncommon in blunt chest trauma. Many of these patients die before reaching the hospital. In the majority of survivors diagnosis is occasionally delayed resulting in complications like airway stenosis and lung collapse. Thus it is important to have radiological follow up after severe thoracic trauma. Sleeve resection can be an excellent option to conserve lung tissue in delayed presentation of bronchial transection.

  13. Sampling forest tree regeneration with a transect approach

    Directory of Open Access Journals (Sweden)

    D. Hessenmöller

    2013-05-01

    Full Text Available A new transect approach for sampling forest tree regeneration isdeveloped with the aim to minimize the amount of field measurements, and to produce an accurate estimation of tree species composition and density independent of tree height. This approach is based on the “probability proportional to size” (PPS theory to assess heterogeneous vegetation. This new method is compared with other approaches to assess forest regeneration based on simulated and measured, real data. The main result is that the transect approach requires about 50% of the time to assess stand density as compared to the plot approach, due to the fact that only 25% of the tree individuals are measured. In addition, tall members of the regeneration are counted with equal probability as small members. This is not the case in the plot approach. The evenness is 0.1 to 0.2 units larger in the transect by PPS than in the plot approach, which means that the plot approach shows a more homogeneous regeneration layer than the PPS approach, even though the stand densities and height distributions are similar. The species diversity is variable in both approaches and needs further investigations.

  14. Transect-based Three-Dimensional Road Modeling and Visualization

    Institute of Scientific and Technical Information of China (English)

    LI Qingquan; TANG Luliang; ZUO Xiaoqing; LI Hanwu

    2004-01-01

    On the basis of the study on road elements and the existing three-dimensional road data models, this paper puts forward the transect-based road modeling, resolves the modeling of the simplest road with two road transects, and fulfils the visualization of the road. OpenGL is applied as the tool of visualization. The map texture technology, LOD algorithm and dynamic multi-differentiating texture technology are of benefit to the construction of 3D road GIS, and improve the speed of roaming and effective visualization. This paper also disusses the combination of 2D digitall road map and 3D road scene, and the dynamic response between them. Finally, on the basis of the research on the transect-based road model, this paper develops the three-dimensional road geographic information system called virtual road ( VRoad ), which not only supplies road designers with a set of tool which can turn the designed 2D road data into 3D road and the high road assistant function area in computer, but also supplies the road management with a set of tool which can realize the road real time and interactive roaming, high-efficiency management.

  15. Liver trauma and transection of the inferior vena cava

    International Nuclear Information System (INIS)

    CT of a child with severe liver trauma due to a seat belt injury demonstrated avulsion of a portion of the lateral segment of the left lobe of the liver. The location of nondependent extravasated contrast material aided in identification of the visceral fracture site (the sentinel contrast sign). Associated transection of the inferior vena cava was evidenced by hypoatenuating zones adjacent to all the major hepatic veins and vena cava (hepatic perivenous tracking). Recognition of these two signs is important so that the radiologist can help the surgeon select the optimal operative approach. (orig.)

  16. Application of Golden Section Method to Channel Transect Design

    Institute of Scientific and Technical Information of China (English)

    2001-01-01

    During the desing of channel transect,the paper brings forward golden section method,which is 0. 618methods. In order to reduce the calculation volume of the natural depth of water ho and bottom-width b which apply trial calculation method and gralphic method ,and improve the calculate precision ,the mathematical model has been built up,the writer combines example to explain the train of thought ,the result shows that the calculation precision is high, the correctness is tested and verified by the result which is calculated by hand. It can be referred tO the hydroelectric works.

  17. Radon and ammonia transects across the Cerro Prieto geothermal field

    Energy Technology Data Exchange (ETDEWEB)

    Semprini, L.; Kruger, P.

    1981-01-01

    Radon and ammonia transects, conducted at the Cerro Prieto geothermal field, involve measurement of concentration gradients at wells along lines of structural significance in the reservoir. Analysis of four transects showed radon concentrations ranging from 0.20 to 3.60 nCi/kg and ammonia concentrations from 17.6 to 59.3 mg/l. The data showed the lower concentrations in wells of lowest enthalpy fluid and the higher concentrations in wells of highest enthalpy fluid. Linear correlation analysis of the radon-enthalpy data indicated a strong relationship, with a marked influence by the two-phase conditions of the produced fluid. It appears that after phase separation in the reservoir, radon achieves radioactive equilibrium between fluid and rock, suggesting that the phase separation occurs well within the reservoir. A two-phase mixing model based on radon-enthalpy relations allows estimation of the fluid phase temperatures in the reservoir. Correlations of ammonia concentration with fluid enthalpy suggests an equilibrium partitioning model in which enrichment of ammonia correlates with higher enthalpy vapor.

  18. Terrestrial transect study on driving mechanism of vegetation changes

    Institute of Scientific and Technical Information of China (English)

    ZHANG YuJin; ZHOU GuangSheng

    2008-01-01

    In terms of Chinese climate-vegetation model based on the classification of plant functional types,together with climatic data from 1951 to 1980 and two future climatic scenarios (SRES-A2 and SRES-B2)in China from the highest and the lowest emission scenarios of greenhouse gases,the distribution patterns of vegetation types and their changes along the Northeast China Transect (NECT) and the North-South Transect of Eastern China (NSTEC) were simulated in order to understand the driving mechanisms of vegetation changes under climatic change. The results indicated that the vegetation distribution patterns would change significantly under future climate,and the major factors driving the vegetation changes were water and heat. However,the responses of various vegetation types to the changes in water and heat factors were obviously different. The vegetation changes were more sensitive to heat factors than to water factors. Thus,in the future climate warming will significantly affect vegetation distribution patterns.

  19. Bilateral Traumatic Globe Luxation with Optic Nerve Transection

    Directory of Open Access Journals (Sweden)

    Levent Tok

    2014-12-01

    Full Text Available Purpose: The purpose of this study was to document clinical findings and management of a patient with bilateral globe luxation and optic nerve transection. Materials and Methods: A 25-year-old female patient was admitted to the emergency department with bilateral traumatic globe luxation following a motor vehicle accident. Results: Visual acuity testing showed no light perception. The right pupil was dilated and bilaterally did not react to light. The globes were bilaterally intact. A computed tomography scan revealed Le Fort type II fractures, bilateral optic nerve transection and disruption of all extraocular muscles. The globes of the patient were bilaterally reduced into the orbit. However, the patient developed phthisis bulbi in the right eye at month 3. Conclusion: Globe luxation presents a dramatic clinical picture, and may lead to the development of severe complications due to the concomitance of complete optic nerve dissection and multiple traumas. Even if the luxated globe is repositioned into the orbit, there is still an increased risk of the development of phthisis due to ischemia.

  20. Image-guided ureteral reconstruction using rendezvous technique for complex ureteric transection after gunshot injuries.

    Science.gov (United States)

    Arabi, Mohammad; Mat'hami, Abdulaziz; Said, Mohammad T; Bulbul, Muhammad; Haddad, Maurice; Al-Kutoubi, Aghiad

    2016-01-01

    Management of complex ureteric transection poses a significant clinical challenge, particularly after gunshot injuries due to marked distortion of anatomy and associated tissue loss. We report two cases of total ureteric transection due to gunshot injury successfully repaired using fluoroscopy-guided rendezvous procedure and double J stent placement. This minimally invasive approach may offer a safe and effective technique to repair complete ureteral transection and obviate the need for complex surgical procedures. PMID:26955601

  1. Surgical treatment for traumatic transection of left common carotid artery

    Institute of Scientific and Technical Information of China (English)

    ZHONG Qian-jin; XIAO Ying-bin; PENG Li; HAO Jia; AN Yong; MA Rui-yan

    2005-01-01

    @@ Injury to the supraaortic artery is a rare event, with poor prognosis and high mortality.1 Improvement of the outcome may lie on the combination of several aspects, including better pre-hospital care, use of emergency cardiopulmonary bypass (CPB), improved surgical techniques and facilities, and advanced postoperative intensive care. Some researchers emphasized the importance of emergency CPB in the treatment and thought that it was responsible mainly for the improved outcome.2-6 However, there exists controversies about it.7-9 In this article, we reported that a patient with life-threatening hemorrhage due to traumatic transection of the left common carotid artery, who was admitted to our hospital in July 2003, was treated successfully with operations with help of emergency CPB and systemic hypothermia.

  2. Carbon balance along the Northeast China Transect(NECT-IGBP)

    Institute of Scientific and Technical Information of China (English)

    周广胜; 王玉辉; 蒋延玲; 许振柱

    2002-01-01

    The Northeast China Transect (NECT) along a precipitation gradient wasused to calculate the carbon balance of different vegetation types, land-use practices and temporal scales. NECT consists of mixed coniferous-broadleaved forest ecosystems, meadow steppe ecosystems and typical steppe ecosystems. Analyses of the C budget were carried out with field measurement based on dark enclosed chamber techniques and alkali absorption methods, and the application of the CENTURY model. Results indicated that: (1) soil CO2 flux had a strong diurnal and seasonal variation influenced by grassland type and land-use practices. However, the seasonal variation on soil CO2 fluxes did not show obvious changes between non-grazing and grazing Leymus chinensis dominated grasslands. (2) Hourly soil CO2 fluxes mainly depended on temperature, while daily CO2 fluxes were affected bothby temperature and moisture. (3) NPP of the three typical ecosystems showed linear relationships with inter-annual precipitation, but total soil carbon of those ecosystems did not. NPP and total soil carbon values decreased westward with decreasing precipitation. (4) Model simulation of NPP and total soil carbon showed that mean annual precipitation was the major limiting factor for ecosystem productivity along NECT. (5) Mean annual carbon budget is the largest for the mixedconiferous- broadleaved forest ecosystem (503.2 gC m-2 a-1), followed by the meadow steppe ecosystem (227.1 gC m-2 a-1), and the lowest being the typical steppe ecosystem (175.8 gC m-2 a-1). This study shows that concurrent field measurements of terrestrial ecosystems including the soil and plant systems with surface layer measurements along the water-driven IGBP-NECT are valuable in understanding the mechanisms driving the carbon cycle in different vegetation types under different land-use practices. Future transect research should be emphasized.

  3. Composition and diversity of tree species in transects of location lowland evergreen forest of Ecuador

    Directory of Open Access Journals (Sweden)

    Jorge Caranqui A.

    2015-09-01

    Full Text Available The study was conducted in 9 transects 1000m2 of lowland evergreen forest, located in two locations on the coast and one in eastern Ecuador. It was to contribute to knowledge of the diversity and composition of woody plants over 10 cm diameter at breast height (DBH plus infer the state of conservation of forests based on the composition, the number of species, indices diversity and importance value (IV, found in 9 transects of 1000 m² of forest: 156 species, 107 genera and 39 families distributed in 9 transects, in each one the Simpson diversity index is of 0.92 to 0.95, in this case are diversity because all approaches 1. Most were found species aren´t present in all transects, the index value in each transect does not exceed 40%. Grouping transects match three locations exception made to transect 5 and 8 were conducted in disturbed sites, the most transects are intermediate disturbance that their high levels of diversity.

  4. Harness Traction Technique (HARNESS): Novel Method for Controlling the Transection Plane During Laparoscopic Hepatectomy.

    Science.gov (United States)

    Itano, Osamu; Oshima, Go; Kitago, Minoru; Suzuki, Keiichi; Hayatsu, Shigeo; Shinoda, Masahiro; Abe, Yuta; Hibi, Taizo; Yagi, Hiroshi; Ikoma, Naruhiko; Aiko, Satoshi; Kitagawa, Yuko

    2015-08-01

    We present our experience using a novel method for controlling the transection plane, which we termed as the Harness Traction Technique (HARNESS) and evaluate its usefulness. From May 2009 to March 2012, laparoscopic hepatectomies using HARNESS were performed on 35 patients. After the superficial hepatic parenchyma on the line was transected at 1 to 2 cm depth, 5 mm tape was placed along the groove of the line and tied to prevent it from slipping off. Tape was tied and pulled using a forceps toward the best direction for minimizing the bleeding, moving the transection point to the appropriate position and creating good tension for parenchymal transection at the transection point. There were no conversions to laparotomy or intraoperative complications. HARNESS is useful for controlling the dissection line during laparoscopic hepatectomy, leading to precise and safe laparoscopic liver parenchymal dissection. PMID:26121541

  5. The Characteristics of Plant Communities Along East Eurasian Steppe Transect

    Institute of Scientific and Technical Information of China (English)

    HAN Wen-jun; HOU Xiang-yang; OLOKHNUUD Chun-liang; Michael P Schellenberg

    2014-01-01

    The East Eurasian Steppe Transect (EEST) is the ifrst international transect across regions of middle and high latitudes in the eastern Eurasian steppe. The EEST is an ideal platform for researching the response of Eurasian temperate steppe to global change, because of its integrated gradients of temperature and human activities on a large-scale. In this study, basic characteristics of plant communities along the EEST across a latitudinal gradient was analyzed. According to the survey of 58 sampling sites, there are 140 species belonging to 34 families and 94 genera. Of particular note was the ifnding of Astragalus dalaiensis which has disappeared in the grasslands of China. On the whole, Gramineae plants are dominant with Liliaceae plants in the communities signiifcantly decreasing along the latitudinal gradient from south to north. The Shannon-Wiener index and biomass of communities all decreased along the latitudinal gradient with signiifcant negative linear regressions. The SDR2 (summed dominance ratio based on two factors) of dominant plants in the upper layers of communities, such as Stipa and Leymus chinensis, decreased along the latitudinal gradient from south to north. Especially, the SDR2 of L. chinensis decreased signiifcantly. The SDR2 of Cleistogenes squarrosa, Agropyron cristatum in the lower layers of communities and the indicator species for degradation were not affected. Potentilla acaulis was found mainly in the southern and northern areas. Stellera chamaejasme was found just in a few sites in the southern area of the EEST. In communities of Stipa grandis and Stipa krylovii, annual and biennial species are dominant. The ratio of annual and biennial species in the community is signiifcantly related to the latitudinal gradient. Perennial herbaceous plants and shrubs were not affected. According to the principal component analysis (PCA), with the data from 58×140 dimensions, the ifrst and second components had the lowest proportion, thus indicating

  6. Shrinkage of ipsilateral taste buds and hyperplasia of contralateral taste buds following chorda tympani nerve transection

    Institute of Scientific and Technical Information of China (English)

    Yi-ke Li; Juan-mei Yang; Yi-bo Huang; Dong-dong Ren; Fang-lu Chi

    2015-01-01

    The morphological changes that occur in the taste buds after denervation are not well under-stood in rats, especially in the contralateral tongue epithelium. In this study, we investigated the time course of morphological changes in the taste buds following unilateral nerve transection. The role of the trigeminal component of the lingual nerve in maintaining the structural integrity of the taste buds was also examined. Twenty-four Sprague-Dawley rats were randomly divided into three groups:control, unilateral chorda tympani nerve transection and unilateral chorda tympani nerve transection+lingual nerve transection. Rats were allowed up to 42 days of re-covery before being euthanized. The taste buds were visualized using a cytokeratin 8 antibody. Taste bud counts, volumes and taste receptor cell numbers were quantiifed and compared among groups. No signiifcant difference was detected between the chorda tympani nerve transection and chorda tympani nerve transection+lingual nerve transection groups. Taste bud counts, vol-umes and taste receptor cell numbers on the ipsilateral side all decreased signiifcantly compared with control. On the contralateral side, the number of taste buds remained unchanged over time, but they were larger, and taste receptor cells were more numerous postoperatively. There was no evidence for a role of the trigeminal branch of the lingual nerve in maintaining the structural integrity of the anterior taste buds.

  7. Shrinkage of ipsilateral taste buds and hyperplasia of contralateral taste buds following chorda tympani nerve transection

    Directory of Open Access Journals (Sweden)

    Yi-ke Li

    2015-01-01

    Full Text Available The morphological changes that occur in the taste buds after denervation are not well understood in rats, especially in the contralateral tongue epithelium. In this study, we investigated the time course of morphological changes in the taste buds following unilateral nerve transection. The role of the trigeminal component of the lingual nerve in maintaining the structural integrity of the taste buds was also examined. Twenty-four Sprague-Dawley rats were randomly divided into three groups: control, unilateral chorda tympani nerve transection and unilateral chorda tympani nerve transection + lingual nerve transection. Rats were allowed up to 42 days of recovery before being euthanized. The taste buds were visualized using a cytokeratin 8 antibody. Taste bud counts, volumes and taste receptor cell numbers were quantified and compared among groups. No significant difference was detected between the chorda tympani nerve transection and chorda tympani nerve transection + lingual nerve transection groups. Taste bud counts, volumes and taste receptor cell numbers on the ipsilateral side all decreased significantly compared with control. On the contralateral side, the number of taste buds remained unchanged over time, but they were larger, and taste receptor cells were more numerous postoperatively. There was no evidence for a role of the trigeminal branch of the lingual nerve in maintaining the structural integrity of the anterior taste buds.

  8. Form, quantity, and fate of nitrogen inputs along a boreal forest climate transect

    Science.gov (United States)

    VandenBoer, T. C.; Edwards, K.; Ziegler, S. E.

    2013-12-01

    The cycling and fate of soil organic matter, a globally significant carbon (C) reservoir, is intimately linked to the availability and form of nitrogen (N). Nitrogen inputs to remote ecosystems from the atmosphere have been accelerated by increases in agricultural fertilizer use, and fossil fuel use. Such inputs may influence the biogeochemistry of high latitude ecosystems where soil organic matter reservoirs are particularly vulnerable to climate change. The Newfoundland and Labrador Boreal Ecosystem Latitudinal Transect (NL-BELT) network has been actively monitoring C and N pools across three watershed regions spanning 47° to 53° N to understand boreal forest ecosystem responses to a warming climate. Litterfall inputs exhibited decreasing stable nitrogen isotope (d15N) values from south to north along the transect (-2, -3, and -6 ‰) and also among litter sources (deciduous > green needles > brown needles). These regional trends in d15N persist in the bryophytic biomass and throughout the soil organic horizons. Litterfall C:N increased from approximately 55 in the south to 75 in the north. In each region, C:N also exhibited consistent patterns between litterfall input types, with C:N of brown needles > deciduous > green needles. Differences between green and brown needle litterfall C:N increased along this climate gradient indicating that trees increased their N-resorption from south to north, perhaps due to increased N-limitation. Two possible phenomena could explain the trends observed: i) atmospheric N-inputs vary in quantity and composition with latitude along the transect; and/or ii) more rapid recycling of N at the most southern site reduces N-limitation relative to cooler regions. Fractionation during long-range transport of atmospheric reactive-N leads to depletion of 15N and subsequent deposition to the NL-BELT regions could explain these observations. The forms and quantities of atmospheric N-inputs are not constrained for the NL-BELT forests and

  9. Aerobic Methane Oxidation in Alaskan Lakes Along a Latitudinal Transect

    Science.gov (United States)

    Martinez-Cruz, K. C.; Sepulveda-Jauregui, A.; Walter Anthony, K. M.; Anthony, P.; Thalasso, F.

    2013-12-01

    Karla Martinez-Cruz* **, Armando Sepulveda-Jauregui*, Katey M. Walter Anthony*, Peter Anthony*, and Frederic Thalasso**. * Water and Environmental Research Center, Institute of Northern Engineering, University of Alaska Fairbanks, Fairbanks, Alaska. ** Biotechnology and Bioengineering Department, Cinvestav, Mexico city, D. F., Mexico. Methane (CH4) is the third most important greenhouse gas in the atmosphere, after carbon dioxide and water vapor. Boreal lakes play an important role in the current global warming by contributing as much as 6% of global atmospheric CH4 sources annually. On the other hand, aerobic methane oxidation (methanotrophy) in lake water is a fundamental process in global methane cycling that reduces the amount of CH4 emissions to the atmosphere. Several environmental factors affect aerobic methane oxidation in the water column both directly and indirectly, including concentration of CH4 and O2, temperature and carbon budgets of lakes. We analyzed the potential of aerobic methane oxidation (PMO) rates in incubations of water collected from 30 Alaskan lakes along a north-south transect during winter and summer 2011. Our findings showed an effect of CH4 and O2 concentrations, temperature and yedoma thawing permafrost on PMO activity in the lake water. The highest PMO rates were observed in summer by lakes situated on thawing yedoma permafrost, most of them located in the interior of Alaska. We also estimated that 60-80% of all CH4 produced in Alaskan lakes could be taken up by methanotrophs in the lake water column, showing the significant influence of aerobic methane oxidation of boreal lakes to the global CH4 budget.

  10. Artificial ultra-fine aerosol tracers for highway transect studies

    Science.gov (United States)

    Cahill, Thomas A.; Barnes, David E.; Wuest, Leann; Gribble, David; Buscho, David; Miller, Roger S.; De la Croix, Camille

    2016-07-01

    The persistent evidence of health impacts of roadway aerosols requires extensive information for urban planning to avoid putting populations at risk, especially in-fill projects. The required information must cover both highway aerosol sources as well as transport into residential areas under a variety of roadway configurations, traffic conditions, downwind vegetation, and meteorology. Such studies are difficult and expensive to do, but were easier in the past when there was a robust fine aerosol tracer uniquely tied to traffic - lead. In this report we propose and test a modern alternative, highway safety flare aerosols. Roadway safety flares on vehicles in traffic can provide very fine and ultra-fine aerosols of unique composition that can be detected quantitatively far downwind of roadways due to a lack of upwind interferences. The collection method uses inexpensive portable aerosol collection hardware and x-ray analysis protocols. The time required for each transect is typically 1 h. Side by side tests showed precision at ± 4%. We have evaluated this technique both by aerosol removal in vegetation in a wind tunnel and by tracking aerosols downwind of freeways as a function of season, highway configuration and vegetation coverage. The results show that sound walls for at-grade freeways cause freeway pollution to extend much farther downwind than standard models predict. The elevated or fill section freeway on a berm projected essentially undiluted roadway aerosols at distances well beyond 325 m, deep into residential neighborhoods. Canopy vegetation with roughly 70% cover reduced very fine and ultra-fine aerosols by up to a factor of 2 at distances up to 200 m downwind.

  11. A summary of the 2004 Devils Lake Wetland Management District roadside transect survey

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — This report is on the 2004 road transect surveys conducted on the Devils Lake Wetland Management District. Surveys were preformed in an effort to fill the data gap...

  12. [Endovascular repair for an acute traumatic aortic transection: a case report].

    Science.gov (United States)

    Sanioğlu, Soner; Sahin, Sinan; Aydoğan, Hakki; Barutça, Hakan; Eren, Ergin

    2012-03-01

    A thirty-eight-year-old male patient who suffered from 10th and 11th thoracal vertebrae fractures, paraplegia and acute traumatic aortic transection because of accidental fall was referred to our hospital. Open surgical repair carried a very high risk due to severe coexisting injuries. Transection was treated with 30x100 mm Valiant thoracic endograft, which was deployed just distal to the ostium of the left carotid artery. The patient was transferred to the neurosurgery clinic for treatment of paraplegia after an uneventful recovery. Endovascular repair of acute transection confers substantial advantages in mortality and morbidity compared to surgical repair. However, the long-term durability of thoracic endografts remains unknown. If the long-term results are as satisfactory as the promising mid-term results, this technique may become the gold standard approach for the treatment of acute transection. PMID:22792827

  13. Brain-derived neurotrophic factor and neural plasticity in a rat model of spinal cord transection

    Institute of Scientific and Technical Information of China (English)

    Ruxin Xing; Jia Liu; Hua Jin; Ping Dai; Tinghua Wang

    2011-01-01

    The present study employed a rat model of T10 spinal cord transection. Western blot analyses revealed increased brain-derived neurotrophic factor (BDNF) expression in spinal cord segments caudal to the transection site following injection of replication incompetent herpes simplex virus vector (HSV-BDNF) into the subarachnoid space. In addition, hindlimb locomotor functions were improved. In contrast, BDNF levels decreased following treatment with replication defective herpes simplex virus vector construct small interference BDNF (HSV-siBDNF). Moreover, hindlimb locomotor functions gradually worsened. Compared with the replication incompetent herpes simplex virus vector control group, extracellular signal regulated kinase1/2 expression increased in the HSV-BDNF group on days 14 and 28 after spinal cord transection, but expression was reduced in the HSV-siBDNF group. These results suggested that BDNF plays an important role in neural plasticity via extracellular signal regulated kinase1/2 signaling pathway in a rat model of adult spinal cord transection.

  14. Ground Validation Transects (2012) in the U.S. Virgin Islands

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This shapefile denotes the location of underwater photos and video transects taken between 10/23-10/31/12 in Fish Bay and Coral Bay, St. John and the St. Thomas...

  15. Tree Transect Starting Locations (Points) at Sand Creek Massacre National Historic Site, Colorado

    Data.gov (United States)

    National Park Service, Department of the Interior — A vector point dataset representing the starting location of tree transects at Sand Creek Massacre NHS as part of a University of Colorado research study.

  16. A quantitative morphological study of the recovery of cat lingual nerves after transection or crushing.

    OpenAIRE

    Holland, G R; Robinson, P P; Smith, K. G.; Pehowich, E

    1996-01-01

    The morphological changes were examined proximal and distal to crush and transection injuries of the lingual/chorda tympani nerve. Under general anaesthesia the nerve was transected unilaterally in 6 adult cats and crushed with watchmakers forceps in 6 others. After 12 wk, again under general anaesthesia, the injured and contralateral (control) nerves were removed, fixed and embedded for histological examination. Sections were cut from sites proximal and distal to the injury and from a site e...

  17. Ammonia-Oxidizing Bacteria along Meadow-to-Forest Transects in the Oregon Cascade Mountains†

    OpenAIRE

    Mintie, A. T.; Heichen, R. S.; Cromack, Jr., K.; Myrold, D. D.; Bottomley, P. J.

    2003-01-01

    Although nitrification has been well studied in coniferous forests of Western North America, communities of NH3-oxidizing bacteria in these forests have not been characterized. Studies were conducted along meadow-to-forest transects at two sites (Lookout and Carpenter) in the H. J. Andrews Experimental Forest, located in the Cascade Mountains of Oregon. Soil samples taken at 10- or 20-m intervals along the transects showed that several soil properties, including net nitrogen mineralization an...

  18. Low-intensity pulsed ultrasound accelerates nerve regeneration following inferior alveolar nerve transection in rats.

    Science.gov (United States)

    Sato, Mai; Motoyoshi, Mitsuru; Shinoda, Masamichi; Iwata, Koichi; Shimizu, Noriyoshi

    2016-06-01

    Inferior alveolar nerve (IAN) injury, which is frequently caused by orofacial surgery or trauma, induces sensory loss in orofacial regions innervated by the IAN. However, no effective treatment for orofacial sensory loss currently exists. We determined whether sensory loss in facial skin above the mental foramen following IAN transection was recovered by exposure of the transected IAN to low-intensity pulsed ultrasound (LIPUS). Inferior alveolar nerve transection (IANX) was performed in 7-wk-old male Sprague-Dawley rats. On day 7 after IANX, the effect of daily LIPUS (from day 0) on the transected IAN, in terms of sensitivity to mechanical stimulation of the facial skin above the mental foramen, was examined. Moreover, the number of trigeminal ganglion (TG) neurons innervating the facial skin above the mental foramen of rats with IANX treated daily with LIPUS was counted using the retrograde neurotracing technique. Daily exposure of the transected IAN to LIPUS significantly promoted recovery of the head-withdrawal threshold in response to mechanical stimulation of the facial skin above the mental foramen, and the number of TG neurons innervating the facial skin above mental foramen was significantly increased in rats with IANX treated daily with LIPUS compared with sham or LIPUS-unexposed rats. Daily treatment of stumps of the transected IAN with LIPUS facilitated morphological and functional regeneration, suggesting that LIPUS is an effective and novel therapy for IAN injury. PMID:27058986

  19. Medusahead invasion along unimproved roads, animal trails, and random transects

    Science.gov (United States)

    Medusahead, an exotic annual grass, is rapidly spreading and causing ecological damage across the western United States. It is critical that land managers prioritize where they direct treatment and monitoring efforts due to the vast areas this exotic plant occupies and the limited amount of resourc...

  20. Quantifying the Spatiotemporal Patterns of Urbanization along Urban-Rural Gradient with a Roadscape Transect Approach: A Case Study in Shanghai, China

    Directory of Open Access Journals (Sweden)

    Zhonghao Zhang

    2016-08-01

    Full Text Available Quantifying the landscape pattern change can effectively demonstrate the ecological progresses and the consequences of urbanization. Based on remotely sensed land cover data in 1994, 2000, 2006 and a gradient analysis with landscape metrics at landscape- and class- level, we attempted to characterize the individual and entire landscape patterns of Shanghai metropolitan during the rapid urbanization. We highlighted that a roadscape transect approach that combined the buffer zone method and the transect-based approach was introduced to describe the urban-rural patterns of agricultural, residential, green, industrial, and public facilities land along the railway route. Our results of landscape metrics showed significant spatiotemporal patterns and gradient variations along the transect. The urban growth pattern in two time spans conform to the hypothesis for diffusion–coalescence processes, implying that the railway is adaptive as a gradient element to analyze the landscape patterns with urbanization. As the natural landscape was replaced by urban landscape gradually, the desakota region expanded its extent widely. Suburb areas witnessed the continual transformation from the predominantly rural landscape to peri-urban landscape. Furthermore, the gap between urban and rural areas remained large especially in public service. More reasonable urban plans and land use policies should push to make more efforts to transition from the urban-rural separation to coordinated urban-rural development. This study is a meaningful trial in demonstrating a new form of urban–rural transects to study the landscape change of large cities. By combining gradient analysis with landscape metrics, we addressed the process of urbanization both spatially and temporally, and provided a more quantitative approach to urban studies.

  1. Location and description of transects for ecological studies in floodplain forests of the lower Suwannee River, Florida

    Science.gov (United States)

    Lewis, L.J.; Light, H.M.; Darst, M.R.

    2001-01-01

    Twelve transects were established in floodplain forests along the lower Suwannee River, Florida, as the principal data collection sites for a comprehensive study conducted by the U.S. Geological Survey and the Suwannee River Water Management District from 1996 to 2001. Data collected along the 12 transects included hydrologic conditions, land-surface elevations, soils, and vegetation of floodplain forests in relation to river flow. Transect locations are marked in the field with permanent markers at approximately 30 meter intervals. Detailed descriptions of the 12 transects and their locations are provided so that they can be used for future ecological studies. Descriptions of the transects include contact information necessary for access to the property on which the transects are located, maps showing transect locations and routes from the nearest city or major road, small scale maps of each transect showing marker locations, latitude and longitude of each marker, compass bearings of each transect line and graphs showing land-surface elevations of the transect with marker locations.

  2. Full spinal cord regeneration after total transection is not possible due to entropy change.

    Science.gov (United States)

    Zielinski, P; Sokal, P

    2016-09-01

    Transected spinal cord regeneration is a main challenge of regenerative medicine. The mainstream of research is focused on the promotion of spinal axons growth, which is strongly inhibited in mammals. Assuming that the inhibition of the axonal growth may be ever overcome, the complexity of neural reconnections may be the second serious stand to overcome. Peripheral nerve axons regeneration seem to form a random pattern of their targets reconnections. The hypothesis is that due to the laws of entropy or irreversible information loss the full spinal cord restoration after the transection is not possible. The hypothesis is discussed based on several assumptions. Simplifying the dissertation spinal cord is represented by 2millions of pyramidal axons. After the transection each of these axons has to make a growth and reconnect with exactly matching targets below the transection, in the same number. Axons are guided by neurotrophic factors and afterwards reconnected with neuroplasticity mechanisms. Assuming random reconnections, there are 2,000,000! permutations [Formula: see text] , therefore the chance of ideally random but correct reconnection of pyramidal axons with adequate targets is 1/2,000,000!. Apart from pyramidal axons, there are other axons, like extrapyramidal, sensory and associative. Empirical data and analysis of neurotrophic factors and organogenesis mechanisms may seem to slightly contradict the hypothesis, but strictly adhering to the second law of thermodynamics and entropy laws the full restoration of the transected cord may never be possible. PMID:27515203

  3. The longitudinal epineural incision and complete nerve transection method for modeling sciatic nerve injury

    Directory of Open Access Journals (Sweden)

    Xing-long Cheng

    2015-01-01

    Full Text Available Injury severity, operative technique and nerve regeneration are important factors to consider when constructing a model of peripheral nerve injury. Here, we present a novel peripheral nerve injury model and compare it with the complete sciatic nerve transection method. In the experimental group, under a microscope, a 3-mm longitudinal incision was made in the epineurium of the sciatic nerve to reveal the nerve fibers, which were then transected. The small, longitudinal incision in the epineurium was then sutured closed, requiring no stump anastomosis. In the control group, the sciatic nerve was completely transected, and the epineurium was repaired by anastomosis. At 2 and 4 weeks after surgery, Wallerian degeneration was observed in both groups. In the experimental group, at 8 and 12 weeks after surgery, distinct medullary nerve fibers and axons were observed in the injured sciatic nerve. Regular, dense myelin sheaths were visible, as well as some scarring. By 12 weeks, the myelin sheaths were normal and intact, and a tight lamellar structure was observed. Functionally, limb movement and nerve conduction recovered in the injured region between 4 and 12 weeks. The present results demonstrate that longitudinal epineural incision with nerve transection can stably replicate a model of Sunderland grade IV peripheral nerve injury. Compared with the complete sciatic nerve transection model, our method reduced the difficulties of micromanipulation and surgery time, and resulted in good stump restoration, nerve regeneration, and functional recovery.

  4. Upregulation of S100A4 after spinal cord transection in adult rats

    Institute of Scientific and Technical Information of China (English)

    Kai-hua ZHANG; Shu HAN; Pei-hua LU; Xiao-ming XU

    2004-01-01

    AIM: To investigate whether spinal cord transection induces changes of gene expression of S 100A4 protein.METHODS: In a spinal cord transection model, S 100A4 expression and cellular localization were examined using cDNA microarray, Northern blot, immunohistochemistry, and immunofluorescence double-labeling methods.RESULTS: There was very limited S 100A4 mRNA expression in the control spinal cord. However, S 100A4 mRNA expression was increased significantly in both the rostral and caudal spinal cord segments adjacent to the injury site.Specifically, S100A4 gene expression was substantially increased at d 2, peaked at d 7 and d 14, and remained high up to 28 d post-injury. During its peak expression, S100A4 protein was localized in astrocytes of the spinal cord within 5 mm from the site of spinal transection. CONCLUSION: Spinal cord transection induces prolonged S 100A4 expression at both mRNA and protein levels in areas close to the injury site. Increased expression of S100A4 in astrocytes after spinal cord transection may indicate that this molecule may play a role in astrocytic responses to injury.

  5. A brain-machine-muscle interface for restoring hindlimb locomotion after complete spinal transection in rats.

    Directory of Open Access Journals (Sweden)

    Monzurul Alam

    Full Text Available A brain-machine interface (BMI is a neuroprosthetic device that can restore motor function of individuals with paralysis. Although the feasibility of BMI control of upper-limb neuroprostheses has been demonstrated, a BMI for the restoration of lower-limb motor functions has not yet been developed. The objective of this study was to determine if gait-related information can be captured from neural activity recorded from the primary motor cortex of rats, and if this neural information can be used to stimulate paralysed hindlimb muscles after complete spinal cord transection. Neural activity was recorded from the hindlimb area of the primary motor cortex of six female Sprague Dawley rats during treadmill locomotion before and after mid-thoracic transection. Before spinal transection there was a strong association between neural activity and the step cycle. This association decreased after spinal transection. However, the locomotive state (standing vs. walking could still be successfully decoded from neural recordings made after spinal transection. A novel BMI device was developed that processed this neural information in real-time and used it to control electrical stimulation of paralysed hindlimb muscles. This system was able to elicit hindlimb muscle contractions that mimicked forelimb stepping. We propose this lower-limb BMI as a future neuroprosthesis for human paraplegics.

  6. Water yield responses to climate change and variability across the North-South Transect of Eastern China (NSTEC)

    Science.gov (United States)

    Lu, Nan; Sun, Ge; Feng, Xiaoming; Fu, Bojie

    2013-02-01

    SummaryChina is facing a growing water crisis due to climate and land use change, and rise in human water demand across this rapidly developing country. Understanding the spatial and temporal ecohydrologic responses to climate change is critical to sustainable water resource management. We investigated water yield (WY) responses to historical (1981-2000) and projected potential climate changes across a large and complex climatic and land cover gradients over the North-South Transect of Eastern China (NSTEC, a standard terrestrial transect of the International GeoBiological Project, IGBP). After an annual scale evapotranspiration (ET) model was validated with historical streamflow records from ten watersheds, the model was applied to the NSTEC that encompasses seven climatic zones. We found that (1) The spatial and temporal variations of WY were highly dependent on precipitation (P) patterns during 1981-2000. Overall, the influences of significant temperature (T) rise on the trend of WY were suppressed by the insignificant P change during 1981-2000. (2) The long-term mean WY by climatic zone had a similar pattern as P. The different climatic zones had differential contributions to the total volumetric WY of the NSTEC. Within each climatic zone, the volumetric WY for each land cover type was highly dependent on its area of each land cover. (3) Corresponding to the P pattern, the mean WY decreased from the low (South) to high latitude (North), but the rates of changes varied along the NSTEC. Along the NSTEC, the sensitivity of WY to potential T and P changes increased from the high latitude to the low latitude. Future potential changes in WY are likely to follow changes in P with some modification by changes in energy availability. We conclude that precipitation is a major driver for water resource availability, and reliable prediction of future precipitation change patterns is critical to hydrologic forecast across the study region.

  7. Spatio-Temporal Distribution of Dengue and Lymphatic Filariasis Vectors along an Altitudinal Transect in Central Nepal

    Science.gov (United States)

    Dhimal, Meghnath; Gautam, Ishan; Kreß, Aljoscha; Müller, Ruth; Kuch, Ulrich

    2014-01-01

    Background Rapidly increasing temperatures in the mountain region of Nepal and recent reports of dengue fever and lymphatic filariasis cases from mountainous areas of central Nepal prompted us to study the spatio-temporal distribution of the vectors of these two diseases along an altitudinal transect in central Nepal. Methodology/Principal Findings We conducted a longitudinal study in four distinct physiographical regions of central Nepal from September 2011 to February 2012. We used BG-Sentinel and CDC light traps to capture adult mosquitoes. We found the geographical distribution of the dengue virus vectors Aedes aegypti and Aedes albopictus along our study transect to extend up to 1,310 m altitude in the Middle Mountain region (Kathmandu). The distribution of the lymphatic filariasis vector Culex quinquefasciatus extended up to at least 2,100 m in the High Mountain region (Dhunche). Statistical analysis showed a significant effect of the physiographical region and month of collection on the abundance of A. aegypti and C. quinquefasciatus only. BG-Sentinel traps captured significantly higher numbers of A. aegypti than CDC light traps. The meteorological factors temperature, rainfall and relative humidity had significant effects on the mean number of A. aegypti per BG-Sentinel trap. Temperature and relative humidity were significant predictors of the number of C. quinquefasciatus per CDC light trap. Dengue fever and lymphatic filariasis cases had previously been reported from all vector positive areas except Dhunche which was free of known lymphatic filariasis cases. Conclusions/Significance We conclude that dengue virus vectors have already established stable populations up to the Middle Mountains of Nepal, supporting previous studies, and report for the first time the distribution of lymphatic filariasis vectors up to the High Mountain region of this country. The findings of our study should contribute to a better planning and scaling-up of mosquito

  8. Dark rearing maintains tyrosine hydroxylase expression in retinal amacrine cells following optic nerve transection

    Institute of Scientific and Technical Information of China (English)

    Wei Wan; Zhenghai Liu; Xiaosheng Wang; Xuegang Luo

    2012-01-01

    The present study examined changes in retinal tyrosine hydroxylase (TH) expression in rats having undergone optic nerve transection and housed under a normal day/night cycle or in the dark. The aim was to investigate the effects of amacrine cells on axonal regeneration in retinal ganglion cells and on the synapses that transmit visual signals. The results revealed that retinal TH expression gradually decreased following optic nerve transection in rats housed under a normal day/night cycle, reaching a minimum at 5 days. In contrast, retinal TH expression decreased to a minimum at 1 day following optic nerve transection in dark reared rats, gradually increasing afterward and reaching a normal level at 5-7 days. The number of TH-positive synaptic particles correlated with the TH levels, indicating that dark rearing can help maintain TH expression during the synaptic degeneration stage (5-7 days after optic nerve injury) in retinal amacrine cells.

  9. Endovascular stent graft treatment of acute thoracic aortic transections due to blunt force trauma.

    LENUS (Irish Health Repository)

    Bjurlin, Marc A

    2012-02-01

    Endovascular stent graft treatment of acute thoracic aortic transections is an encouraging minimally invasive alternative to open surgical repair. Between 2006 and 2008, 16 patients with acute thoracic aortic transections underwent evaluation at our institution. Seven patients who were treated with an endovascular stent graft were reviewed. The mean Glasgow Coma Score was 13.0, probability of survival was .89, and median injury severity score was 32. The mean number of intensive care unit days was 7.7, mean number of ventilator support days was 5.4, and hospital length of stay was 10 days. Mean blood loss was 285 mL, and operative time was 143 minutes. Overall mortality was 14%. Procedure complications were a bleeding arteriotomy site and an endoleak. Endovascular treatment of traumatic thoracic aortic transections appears to demonstrate superior results with respect to mortality, blood loss, operative time, paraplegia, and procedure-related complications when compared with open surgical repair literature.

  10. Modelling agricultural suitability along soil transects under current conditions and improved scenario of soil factors

    Science.gov (United States)

    Abd-Elmabod, Sameh K.; Jordán, Antonio; Fleskens, Luuk; van der Ploeg, Martine; Muñoz-Rojas, Miriam; Anaya-Romero, María; van der Salm, Renée J.; De la Rosa, Diego

    2015-04-01

    Agricultural land suitability analysis and improvement of soils by addressing major limitations may be a strategy for climate change adaptation. This study aims to investigate the influence of topography and variability of soil factors on the suitability of 12 annual, semiannual and perennial Mediterranean crops in the province of Seville (southern Spain). In order to represent the variability in elevation, lithology and soil, two latitudinal and longitudinal (S-N and W-E) soil transects (TA and TB) were considered including 63 representative points at regular 4 km intervals. These points were represented by 41 soil profiles from the SDBm soil database -Seville. Almagra model, a component of the agro-ecological decision support system MicroLEIS, was used to assess soil suitability. Results were grouped into five soil suitability classes: S1-optimum, S2-high, S3-moderate, S4-marginal and S5-not suitable. Each class was divided in subclasses according to the main soil limiting factors: depth (p), texture (t), drainage (d), carbonate content (c), salinity (s), sodium saturation (a), and the degree of development of the soil profile (g). This research also aimed to maximize soil potential by improving limiting factors d, c, s and a after soil restoration. Therefore, management techniques were also considered as possible scenarios in this study. The results of the evaluation showed that soil suitability ranged between S1 and S5p - S5s along of the transects. In the northern extreme of transect TA, high content of gravels and coarse texture are limiting factors (soils are classified as S4t) In contrast, the limiting factor in the eastern extreme of transect TB is the shallow useful depth (S5p subclass). The absence of calcium carbonate becomes a limiting factor in some parts of TA. In contrast, the excessive content of calcium carbonate appeared to be a limiting factor for crops in some intermediate points of TB transect. For both transects, soil salinity is the main

  11. Altered prosaposin expression in the rat facial nerve nucleus following facial nerve transection and repair

    Institute of Scientific and Technical Information of China (English)

    Dong Wang; Wenlong Luo; Cuiying Zhou; Jingjing Li

    2009-01-01

    BACKGROUND: Studies have demonstrated that damaged facial nerves synthesize prosaposin to promote repair of facial neurons.OBJECTIVE: To observe time-course changes of prosaposin expression in the facial nerve nucleus of Sprague Dawley rats following facial nerve transection and repair.DESIGN, TIME AND SETTING: A randomized control neuropathological animal experiment was performed in Chongqing Medical University between March 2007 and September 2008.MATERIALS: A total of 48 adult, male, Sprague Dawley rats were selected and randomly divided into transection and transection + end-to-end anastomosis groups (n =24). Rabbit anti-rat prosaposin antibody, instant SABC immunohistochemical kit, and antibody dilution solution were purchased from Wuhan Uscn Science Co., Ltd., China.METHODS: In the transection group, the nerve trunk of the distal retroauricular branch of the left facial nerves was ligated in Sprague Dawley rats, and a 5-mm nerve trunk at the distal end of the ligation site was removed. In the transection + end-to-end anastomosis group, epineurial anastomosis was performed immediately following transection of the left facial nerves. The right facial nerves in the two groups sewed as the normal control group.MAIN OUTCOME MEASURES: The number of prosaposin-positive neurons, as well as intensity of immunostaining in facial nerve nucleus, following transection and end-to-end anastomosis were determined by immunohistochemistry at 1,3, 7, 14, 21, and 35 days after injury.RESULTS: Transection group: transection of facial nerves resulted in increased number of prosaposin-positive neurons and immunoreactivity intensity in the facial nucleus on day 1. These values significantly increased by day 3. Expression was greater than in the control side. The peak of the reduction was reached at 7 days post-surgery. Transection + end-to-end anastomosis group: the number of prosaposin-positive neurons and immunoreactivity intensity was reduced in the facial nerve nucleus following

  12. Q value structure of geoscience transect from Korla to Jimsar and its geodynamic implication

    Institute of Scientific and Technical Information of China (English)

    ZHAO; Junmeng(赵俊猛); FAN; Jichang(樊吉昌); LI; Zhichun(李植纯)

    2003-01-01

    In this paper, the methods for Q value inversion in 2-D lateral inhomogeneous medium have been introduced. The 2-D Q value inversion has been conducted using seismic wide-angle reflection/refraction data of the profile from Korla to Jimsar. The result shows that the 2-D Q value structure of the transect from Korla to Jimsar is characterized by vertical stratifying and lateral zoning. Vertically, the crust can be divided into upper crust, middle crust and lower crust with the Q value increasing downwards. Horizontally, the total transect can be classified as three regions--the northern margin of the Tarim Basin, the Tianshan orogenic belt (TOB) and the southern margin of the Junggar Basin. At the northern margin of the Tarim Basin the Q value increases northwards, indicating a northward subduction of the Tarim Basin (TB) into the TOB. The Q value within the TOB jumps near Kumux, making a stage-like difference in Q value. The Q value distribution at the southern margin of the JB suggests a southward subduction of the Junggar Basin (JB) into the TOB.The double subduction pattern of the TB and JB into the TOB revealed by the transect from Korla to Jimsar has a big difference from the model " lithospheric subduction with intrusion of the layers into the crust" developed according to the results of the geoscience transect from Xayar to Burjing. The differences between the two provide some dynamic evidence at lithospheric scale for the segmentation of the TOB.

  13. Relative spatial soil geochemical variability along two transects across the United States and Canada

    Science.gov (United States)

    Garrett, Robert G.

    2009-01-01

    To support the development of protocols for the proposed North American Soil Geochemical Landscapes project, whose objective is to establish baselines for the geochemistry of North American soils, two continental-scale transects across the United States and Canada were sampled in 2004. The sampling employed a spatially stratified random sampling design in order to estimate the variability between 40-km linear sampling units, within them, at sample sites, and due to sample preparation and analytical chemical procedures. The 40-km scale was chosen to be consistent with the density proposed for the continental-scale project. The two transects, north–south (N–S) from northern Manitoba to the USA–Mexico border near El Paso, Texas, and east–west (E–W) from the Virginia shore north of Washington, DC, to north of San Francisco, California, closely following the 38th parallel, have been studied individually. The purpose of this study was to determine if statistically significant systematic spatial variation occurred along the transects. Data for 38 major, minor and trace elements in A- and C-horizon soils where less than 5% of the data were below the detection limit were investigated by Analysis of Variance (ANOVA). A total of 15 elements (K, Na, As, Ba, Be, Ce, La, Mn, Nb, P, Rb, Sb, Th, Tl and W) demonstrated statistically significant (p<0.05) variability at the between-40-km scale for both horizons along both transects. Only Cu failed to demonstrate significant variability at the between-40-km scale for both soil horizons along both transects.

  14. A clinical comparative analysis of crush/clamp, stapler, and dissecting sealer hepatic transection methods1

    Science.gov (United States)

    Earl, T. Mark; Chari, Ravi S.; Gorden, D. Lee; Merchant, Nipun B.; Wright, J. Kelly; Feurer, Irene D.; Pinson, C. Wright

    2008-01-01

    Introduction. Several methods for hepatic parenchymal division exist. The primary aim was to assess differences in postoperative bile leaks, operative blood loss, and margin status between three transection methods: crush/clamp (CC), stapler (SP), or dissecting sealer (DS). Methods. A single institution, retrospective cohort study was performed on data collected over a three-year period in patients undergoing elective liver resection using the CC, SP, or DS. Patients were excluded if multiple methods of transection were used or for intraoperative death. The association of bile leak with transection type was assessed. A logistic regression model was tested to assess if blood loss was associated with the covariates of transection method, use of portal inflow occlusion, extent of liver resection, and other concurrent major operations. Results. Analyses included 141 patients. The stapler method was quicker than the other methods (p=0.01). The risk of postoperative bile leak was no different between CC, SP, and DS transection methods (p=0.23). There was no difference in mean blood loss or transfusions; however, hepatectomies performed with DS were associated with an increased risk of blood loss ≥1000 mL compared to CC (p=0.04). There were no differences in mean surgical margin between the three methods. Conclusion. The risk of bile leaks was not different between the three methods. While mean blood loss was similar, hepatectomy performed with the DS was associated with an increased risk of having operative blood loss ≥1000 mL compared to CC. Margins were equal by all methods. The stapler method was quicker. PMID:18982146

  15. Genetic structure of desert ground squirrels over a 20-degree-latitude transect from Oregon through the Baja California peninsula.

    Science.gov (United States)

    Whorley, Joshua R; Alvarez-Castañeda, S Ticul; Kenagy, G J

    2004-09-01

    The genetic structure of populations over a wide geographical area should reflect the demographic and evolutionary processes that have shaped a species across its range. We examined the population genetic structure of antelope ground squirrels (Ammospermophilus leucurus) across the complex of North American deserts from the Great Basin of Oregon to the cape region of the Baja California peninsula. We sampled 73 individuals from 13 major localities over this 2500-km transect, from 43 to 22 degrees north. Our molecular phylogeographical analysis of 555 bp of the mitochondrial cytochrome b gene and 510 bp of the control region revealed great genetic uniformity in a single clade that extends from Oregon to central Baja California. A second distinct clade occupies the southern half of the peninsula. The minimal geographical structure of the northern clade, its low haplotype diversity and the distribution of pairwise differences between haplotypes suggest a rapid northward expansion of the population that must have followed a northward desert habitat shift associated with the most recent Quaternary climate warming and glacial retreat. The higher haplotype diversity within the southern clade and distribution of pairwise differences between haplotypes suggest that the southern clade has a longer, more stable history associated with a southern peninsular refugium. This system, as observed, reflects both historical and contemporary ecological and evolutionary responses to physical environmental gradients within genetically homogeneous populations. PMID:15315683

  16. Stocks, Chemistry, and Sensitivity to Climate Change of Dead Organic Matter Along the Canadian Boreal Forest Transect Case Study

    Energy Technology Data Exchange (ETDEWEB)

    Preston, C.M.; Norris, C. [Pacific Forestry Centre, Natural Resources Canada, Victoria, BC, V8Z 1M5 (Canada); Bhatti, J.S. [Northern Forestry Centre, Natural Resources Canada, Edmonton, AB, T6H 3S5 (Canada); Flanagan, L.B. [Department of Biological Sciences, University of Lethbridge, Lethbridge, AB, T1K 3M4 (Canada)

    2006-01-15

    Improving our ability to predict the impact of climate change on the carbon (C) balance of boreal forests requires increased understanding of site-specific factors controlling detrital and soil C accumulation. Jack pine (Pinus banksiana) and black spruce (Picea mariana) stands along the Boreal Forest Transect Case Study (BFTCS) in northern Canada have similar C stocks in aboveground vegetation and large woody detritus, but thick forest floors of poorly-drained black spruce stands have much higher C stocks, comparable to living biomass. Their properties indicate hindered decomposition and N cycling, with high C/N ratios, strongly stratified and depleted d13C and d15N values, high concentrations of tannins and phenolics, and 13C nuclear magnetic resonance (NMR) spectra typical of poorly decomposed plant material, especially roots and mosses. The thinner jack pine forest floor appears to be dominated by lichen, with char in some samples. Differences in quantity and quality of aboveground foliar and woody litter inputs are small and unlikely to account for the contrasts in forest floor accumulation and properties. These are more likely associated with site conditions, especially soil texture and drainage, exacerbated by increases in sphagnum coverage, forest floor depth, and tannins. Small changes in environmental conditions, especially reduced moisture, could trigger large C losses through rapid decomposition of forest floor in poorly drained black spruce stands in this region.

  17. A photographic and acoustic transect across two deep-water seafloor mounds, Mississippi Canyon, northern Gulf of Mexico

    Science.gov (United States)

    Hart, P.E.; Hutchinson, D.R.; Gardner, J.; Carney, R.S.; Fornari, D.

    2008-01-01

    In the northern Gulf of Mexico, a series of seafloor mounds lie along the floor of the Mississippi Canyon in Atwater Valley lease blocks 13 and 14. The mounds, one of which was drilled by the Chevron Joint Industry Project on Methane Hydrates in 2005, are interpreted to be vent-related features that may contain significant accumulations of gas hydrate adjacent to gas and fluid migration pathways. The mounds are located ???150 km south of Louisiana at ???1300 m water depth. New side-scan sonar data, multibeam bathymetry, and near-bottom photography along a 4 km northwest-southeast transect crossing two of the mounds (labeled D and F) reveal the mounds' detailed morphology and surficial characteristics. Mound D, ???250 m in diameter and 7-10 m in height, has exposures of authigenic carbonates and appears to result from a seafloor vent of slow-to-moderate flux. Mound F, which is ???400 m in diameter and 10-15 m high, is covered on its southwest flank by extruded mud flows, a characteristic associated with moderate-to-rapid flux. Chemosynthetic communities visible on the bottom photographs are restricted to bacterial mats on both mounds and mussels at Mound D. No indications of surficial gas hydrates are evident on the bottom photographs.

  18. Results of trench perpendicular wide angle seismic transects across the Manila subduction zone offshore southern Taiwan

    Science.gov (United States)

    Eakin, D. H.; McIntosh, K. D.; Van Avendonk, H. J.

    2011-12-01

    Multi-channel seismic reflection and wide-angle seismic data collected in 2009 aboard the R/V Marcus Langseth as part of the TAIGER program delineate the crustal structure of the Manila subduction zone in the northern South China Sea. As part of that project, we recorded marine seismic data using a deployment of ocean-bottom-seismometers (OBS) from the U.S. instrument pool and National Taiwan Ocean University. The region between northern Luzon and southern Taiwan evolves from oceanic subduction to incipient arc-continent collision. This presentation focuses on results of 2 offshore transects across the Manila subduction zone offshore southern Taiwan. Our goal here is to document the transition from pure oceanic subuction in the south to incipient arc-continent collision in the north, an understanding of which is integral for future geodynamic modeling of the advanced arc-continent collision in the north. The northern transect, line T2 is located at 21.4° N and used 30 OBSs. Line T1 was located at 20.5° N and used 27 OBSs across the Manila subduction zone. Data quality is extremely variable due to the local geology and quality of seafloor coupling at each instrument. Preliminary travel-time tomography of transect T2 shows a 10-15 km thick Eurasian crust with crustal velocities of 5-7.5 km/sec entering the Manila trench suggesting thinned continental crust, serpentinized upper mantle, or both in this region. The model shows the accretionary prism to be cored by high velocity material (6-7 km/sec) that may be the result of accretion of crustal material from the subducting Eurasian slab. We also observe asymmetric crustal thickening beneath the Gagua Ridge that is potentially a result of failed subduction of the Philippine Sea Plate westward along the Gagua Ridge. The wide-angle data is complimented by MCS reflection data to constrain sediment thickness, top of the crystalline basement, and moho. Preliminary work is in progress with transect T1 which will be

  19. i-got-u GPS waypoints delineating low-altitude transects over the Arctic Network of national park units and Selawik National Wildlife Refuge, Alaska, July 2013

    Data.gov (United States)

    U.S. Geological Survey, Department of the Interior — GPS waypoints delineating the flight paths for low altitude transects from a i-got-u GPS unit. Transects were conducted from small aircraft over the National Park...

  20. Garmin GPS waypoints delineating low-altitude transects over the Arctic Network of national park units and Selawik National Wildlife Refuge, Alaska, July 2013

    Data.gov (United States)

    U.S. Geological Survey, Department of the Interior — GPS waypoints delineating the flight paths for low altitude transects from a Garmin GPS unit. Transects were conducted from small aircraft over the National Park...

  1. Contrasting alluvial architecture of Late Pleistocene and Holocene deposits along a 120-km transect from the central Po Plain (northern Italy)

    Science.gov (United States)

    Campo, Bruno; Amorosi, Alessandro; Bruno, Luigi

    2016-07-01

    High-resolution investigation of a ~ 120-km-long transect along the course of the modern Po River, northern Italy, revealed marked changes in alluvial architecture across the Pleistocene-Holocene boundary. Along the whole transect, a 20- to 30-m thick sheet-like succession of Late Pleistocene fluvial sands is invariably overlain by silt and clay deposits, with isolated fluvial bodies of Holocene age (cal ka BP). The Holocene succession displays consistent downstream changes in facies architecture: well-drained floodplain deposits are transitional at distal locations to increasingly organic, poorly drained floodplain to swamp facies associations. Thick paludal facies extend continuously up to 60 km landward of the Holocene maximum marine ingression, about 90 km from the modern shoreline. Based on 28 radiocarbon dates, the abrupt change in lithofacies and channel stacking pattern occurred at the transition from the last glacial period to the present interglacial, under conditions of rapid sea-level rise. The architectural change from amalgamated, Late Pleistocene sand bodies to overlying, mud-dominated Holocene units represent an example of chronologically well-constrained fluvial response to combined climate and relative sea-level change. The overall aggradational stacking pattern of individual channel-belt sand bodies indicates that high subsidence rates continuously created accommodation in the Po Basin, even during phases of falling sea level and lowstand.

  2. Metagenomic analysis of lacustrine viral diversity along a latitudinal transect of the Antarctic Peninsula.

    Science.gov (United States)

    Aguirre de Cárcer, Daniel; López-Bueno, Alberto; Alonso-Lobo, Juan M; Quesada, Antonio; Alcamí, Antonio

    2016-06-01

    Environmental viruses constitute the most abundant biological entities on earth, and harbor an enormous genetic diversity. While their strong influence on the ecosystem is widely acknowledged, current knowledge about their diversity and distribution remains limited. Here we present the metagenomic study of viral communities from freshwater bodies located along a transect of the Antarctic Peninsula. These ecosystems were chosen on the basis of environmental and biogeographical variation. The results obtained indicate that the virus assemblages were diverse, and that the larger fraction represented viruses with no close relatives in the databases. Comparisons to existing metaviromes showed that the communities studied were dissimilar to other freshwater viromes including those from the Arctic. Finally, we observed no indication of there being a reduction in either viral richness or diversity estimates with increasing latitude along the studied transect, further adding to the controversy regarding the possible existence of latitudinal gradients of diversity in the microbial world. PMID:27059864

  3. Twenty-one years of mass balance observations along the K-transect, West Greenland

    Directory of Open Access Journals (Sweden)

    R. S. W. van de Wal

    2012-08-01

    Full Text Available A 21-yr record is presented of surface mass balance measurements along the K-transect. The series covers the period 1990–2011. Data are available at eight sites along a transect over an altitude range of 380–1850 m at approximately 67° N in West Greenland. The surface mass balance gradient is on average 3.8 × 10−3 m w.e. m−1, and the mean equilibrium line altitude is 1553 m a.s.l. Only the lower three sites within 10 km of the margin up to an elevation of 700 m experience a significant increasing trend in the ablation over the entire period. Data are available at: doi:10.1594/PANGAEA.779181.

  4. Twenty-one years of mass balance observations along the K-transect, West Greenland

    Directory of Open Access Journals (Sweden)

    R. S. W. van de Wal

    2012-05-01

    Full Text Available A 21-yr record is presented of surface mass balance measurements along the K-transect. The series covers the period 1990–2011. Data are available at 8 sites along a transect over an altitude range of 390–1850 m at approximately 67° N in West Greenland. The surface mass balance gradient is on average 3.8 × 10−3 m w.e. m−1, and the mean equilibrium line altitude is 1553 m a.s.l. Only the lower 3 sites within 10 km of the margin experience a significant increasing trend in the ablation over the entire period. Data are available at: http://doi.pangaea.de/10.1594/PANGAEA.779181.

  5. Tropical rain forest structure, tree growth and dynamics along a 2700-m elevational transect in Costa Rica.

    Directory of Open Access Journals (Sweden)

    David B Clark

    Full Text Available Rapid biological changes are expected to occur on tropical elevational gradients as species migrate upslope or go extinct in the face of global warming. We established a series of 9 1-ha plots in old-growth tropical rainforest in Costa Rica along a 2700 m relief elevational gradient to carry out long-term monitoring of tropical rain forest structure, dynamics and tree growth. Within each plot we mapped, identified, and annually measured diameter for all woody individuals with stem diameters >10 cm for periods of 3-10 years. Wood species diversity peaked at 400-600 m and decreased substantially at higher elevations. Basal area and stem number varied by less than two-fold, with the exception of the 2800 m cloud forest summit, where basal area and stem number were approximately double that of lower sites. Canopy gaps extending to the forest floor accounted for <3% of microsites at all elevations. Height of highest crowns and the coefficient of variation of crown height both decreased with increasing elevation. Rates of turnover of individuals and of stand basal area decreased with elevation, but rates of diameter growth and stand basal area showed no simple relation to elevation. We discuss issues encountered in the design and implementation of this network of plots, including biased sampling, missing key meteorological and biomass data, and strategies for improving species-level research. Taking full advantage of the major research potential of tropical forest elevational transects will require sustaining and extending ground based studies, incorporation of new remotely-sensed data and data-acquisition platforms, and new funding models to support decadal research on these rapidly-changing systems.

  6. Panax notoginseng saponins improve recovery after spinal cord transection by upregulating neurotrophic factors

    OpenAIRE

    Bo Wang; Yu Li; Xuan-peng Li; Yang Li

    2015-01-01

    Saponins extracted from Panax notoginseng are neuroprotective, but the mechanisms underlying this effect remain unclear. In the present study, we established a rat model of thoracic (T 10 ) spinal cord transection, and injected Panax notoginseng saponins (100 mg/kg) or saline 30 minutes after injury. Locomotor functions were assessed using the Basso, Beattie, and Bresnahan (BBB) scale from 1 to 30 days after injury, and immunohistochemistry was carried out in the ventral horn of the spinal co...

  7. Post-LIA glacier changes along a latitudinal transect in the Central Italian Alps

    OpenAIRE

    R. Scotti; F. Brardinoni; Crosta, G.B.

    2014-01-01

    The variability of glacier response to atmospheric temperature rise in different topo-climatic settings is still matter of debate. To address this question in the Central Italian Alps we compile a post-LIA (Little Ice Age) multitemporal glacier inventory (1860-1954-1990-2003-2007) along a latitudinal transect that originates north of the continental divide in the Livigno mountains, and extends south through the Disgrazia and Orobie ranges, encompassing conti...

  8. Post-LIA glacier changes along a latitudinal transect in the Central Italian Alps

    OpenAIRE

    R. Scotti; F. Brardinoni; Crosta, G.B.

    2014-01-01

    The variability of glacier response to atmospheric temperature rise in different topo-climatic settings is still a matter of debate. To address this question in the Central Italian Alps, we compile a post-LIA (Little Ice Age) multitemporal glacier inventory (1860–1954–1990–2003–2007) along a latitudinal transect that originates north of the continental divide in the Livigno Mountains and extends south through the Disgrazia and Orobie ranges, encompassing continental-to-ma...

  9. Benthic Foraminifera along a depth transect in western Bahrain: seasonal variations and environmental assessment

    Science.gov (United States)

    Muhammad, Arslan; Kaminski, Michael; Tawabini, Bassam; Ramadan, Khalid Al; Babalola, Lamidi; Frontalini, Fabrizio

    2015-04-01

    We surveyed living benthic foraminifera in a depth transect off western Bahrain (Arabian Gulf) with the aim of understanding the seasonal population dynamics and environmental parameters. In winter, the population was found to be highest due to the large number of rotaliids, followed by miliolids. In each season, the population was found to increase along the depth transect due to the higher number of juveniles. A strong correlation is observed between foraminiferal population and sediment grain size - the juveniles are most abundant on coarser-sandy substrate. The population decreases in the spring and is lowest in the summer. Finally, the living population recovered again in the autumn with increasing juveniles/adult ratios along the depth transect. Results of species consistency and relative abundance showed that Ammonia was consistent from the shallowest to the deepest station, whereas miliolids started appearing in the deeper stations. The average numbers of Elphidium and Peneroplis also increased along the depth transect. Another seasonal effect is that juveniles of Ammonia tepida are found during each season reflecting its reproduction throughout the year, whereas Brizalina pacifica was only found during spring and autumn. The study of environmental parameters reveals that the site is subjected to eutrophication i.e. nitrates and sulfates, however, pollution by heavy metals and hydrocarbons is not significant. An assessment of 63 heavy metals in sediment samples showed that none of the metals had concentrations higher than the internationally accepted norms, which is further confirmed by values of the Foraminiferal Deformities Index of less than 2%. Likewise, no hydrocarbons were detected in the water or sediment samples. Therefore, it is concluded that the site in Bahrain is not yet adversely affected by human development, and therefore can provide baseline information for future comparison and assessment of foraminiferal assemblages in contaminated zones

  10. The Uniformly Most Powerful Invariant Test for the Shoulder Condition in Point Transect Sampling

    OpenAIRE

    Piero Quatto; Riccardo Borgoni

    2006-01-01

    Estimating population abundance is of primary interest in wildlife population studies. Point transect sampling is a well established methodology for this purpose. The usual approach for estimating the density or the size of the population of interest is to assume a particular model for the detection function (the conditional probability of detecting an animal given that it is at a given distance from the observer). The two most popular models for this function are the half-normal model and th...

  11. On the Uniformly Most Powerful Invariant Test for the Shoulder Condition in Line Transect Sampling

    OpenAIRE

    Riccardo Borgoni; Piero Quatto

    2007-01-01

    In wildlife population studies one of the main goals is estimating the population abundance. Line transect sampling is a well established methodology for this purpose. The usual approach for estimating the density or the size of the population of interest is to assume a particular model for the detection function (the conditional probability of detecting an animal given that it is at a given distance from the observer). Two common models for this function are the half-normal model and the neg...

  12. A clinical comparative analysis of crush/clamp, stapler, and dissecting sealer hepatic transection methods1

    OpenAIRE

    Castaldo, Eric T.; Earl, T. Mark; Chari, Ravi S.; Gorden, D. Lee; Merchant, Nipun B; Wright, J. Kelly; Feurer, Irene D; Pinson, C Wright

    2008-01-01

    Introduction. Several methods for hepatic parenchymal division exist. The primary aim was to assess differences in postoperative bile leaks, operative blood loss, and margin status between three transection methods: crush/clamp (CC), stapler (SP), or dissecting sealer (DS). Methods. A single institution, retrospective cohort study was performed on data collected over a three-year period in patients undergoing elective liver resection using the CC, SP, or DS. Patients were excluded if multiple...

  13. Transect based analysis versus area based analysis to quantify shoreline displacement: spatial resolution issues.

    Science.gov (United States)

    Anfuso, Giorgio; Bowman, Dan; Danese, Chiara; Pranzini, Enzo

    2016-10-01

    Field surveys, aerial photographs, and satellite images are the most commonly employed sources of data to analyze shoreline position, which are further compared by area based analysis (ABA) or transect based analysis (TBA) methods. The former is performed by computing the mean shoreline displacement for the identified coastal segments, i.e., dividing the beach area variation by the segment length; the latter is based on the measurement of the distance between each shoreline at set points along transects. The present study compares, by means of GIS tools, the ABA and TBA methods by computing shoreline displacements recorded on two stretches of the Tuscany coast (Italy): the beaches of Punta Ala, a linear coast without shore protection structures, and the one at Follonica, which is irregular due to the presence of groins and detached breakwaters. Surveys were carried out using a differential global positioning system (DGPS) in RTK mode. For each site, a 4800-m-long coastal segment was analyzed and divided into ninety-six 50-m-long sectors for which changes were computed using both the ABA and TBA methods. Sectors were progressively joined to have a length of 100, 200, 400, and 800 m to examine how this influenced results. ABA and TBA results are highly correlated for transect distance and sector length up to 100 m at both investigated locations. If longer transects are considered, the two methods still produce good correlated data on the smooth shoreline (i.e. at Punta Ala), but correlation became significantly lower on the irregular shoreline (i.e., at Follonica). PMID:27640163

  14. Biogeochemical markers of organic matter along the Wight-Cherbourg transect (central English Channel)

    OpenAIRE

    Ismaili, Mm; Serve, L; Gadel, F.; Lafite, R; Texier, H.

    1999-01-01

    Three cruises on the Wight-Cherbourg transect were undertaken during the FluxManche II Programme in order to study the origin, nature and behaviour of organic matter inputs to the waters of the English Channel. Suspended particulate material (SPM) collected from surface and deep waters at each station was analysed at elemental level for Particulate Organic Carbon and Particulate Organic Nitrogen (POC, PON), at molecular level for main classes of organic compounds using Pyrolysis-Gas Chromatog...

  15. Variations of plant life form diversity along northeast China transect and its direct gradient analysis

    Institute of Scientific and Technical Information of China (English)

    2002-01-01

    Plant life form diversity and its direct gradientanalysis on a larger scale climate change gradient were tested,based on the data from Northeast China transect platform. Theresults showed that the species numbers, life form richness andlife form diversity were relative higher at the eastern forests andthe ecotone between typical vegetation, while those on the meadowgrasslands and typical steppes were lower. Although plant lifeforms can reflect the climate variations, life form diversity isnot consistent with the major global gradient along the NECT.

  16. The uniformly most powerful invariant test for two models of detection function in point transect sampling

    OpenAIRE

    Riccardo Borgoni; Piero Quatto

    2013-01-01

    Estimating population abundance is of primary interest in wildlife population studies. Point transect sampling is a well established methodology for this purpose. The usual approach for estimating the density or the size of the population of interest is to assume a particular model for the detection function (the conditional probability of detecting an animal given that it is at a certain distance from the observer). Two popular models for this function are the half-normal model and the negat...

  17. Vascular endothelial growth factor promotes peripheral nerve regeneration after sciatic nerve transection in rat

    Directory of Open Access Journals (Sweden)

    Mohammadi Rahim

    2013-12-01

    Full Text Available 【Abstract】Objective: To evaluate the local effect of vascular endothelial growth factor (VEGF on transected sciatic nerve regeneration. Methods: Sixty male white Wistar rats were divided into four experimental groups randomly (n=15. In transected group the left sciatic nerve was transected and the stump was fixed to adjacent muscle. In treatment group the defect was bridged using a silicone graft filled with 10 µL VEGF. In silicone group the graft was filled with phosphate-buffered saline. In sham-operated group the sciatic nerve was ex- posed and manipulated. Each group was subdivided into three subgroups with five animals in each and nerve fibers were studied 4, 8 and 12 weeks after operation. Results: Behavioral test, functional study of sciatic nerve, gastrocnemius muscle mass and morphometric indi- ces confirmed a faster recovery of regenerated axons in VEGF group than in silicone group (P<0.05. In immunohistochemi- cal assessment, reactions to S-100 in VEGF group were more positive than that in silicone group. Conclusion: Local administration of VEGF will im- prove functional recovery and morphometric indices of sci- atic nerve. Key words: Peripheral nerves; Nerve regeneration; Sciatic nerve; Vascular endothelial growth factor

  18. Vascular endothelial growth factor promotes peripheral nerve regeneration after sciatic nerve transection in rat

    Institute of Scientific and Technical Information of China (English)

    Rahim Mohammadi; Sima Ahsan; Masoume Masoumi; Keyvan Amini

    2013-01-01

    Objective:To evaluate the local effect of vascular endothelial growth factor (VEGF) on transected sciatic nerve regeneration.Methods:Sixty male white Wistar rats were divided into four experimental groups randomly (n=15).In transected group the left sciatic nerve was transected and the stump was fixed to adjacent muscle.In treatment group the defect was bridged using a silicone graft filled with 10μL VEGF.In silicone group the graft was filled with phosphate-buffered saline.In sham-operated group the sciatic nerve was exposed and manipulated.Each group was subdivided into three subgroups with five animals in each and nerve fibers were studied 4,8 and 12 weeks after operation.Results:Behavioral test,functional study of sciatic nerve,gastrocnemius muscle mass and morphometric indices confirmed a faster recovery of regenerated axons in VEGF group than in silicone group (P<0.05).In immunohistochemical assessment,reactions to S-100 in VEGF group were more positive than that in silicone group.Conclusion:Local administration of VEGF will improve functional recovery and morphometric indices of sciatic nerve.

  19. Links between viruses and prokaryotes throughout the water column along a North Atlantic latitudinal transect.

    Science.gov (United States)

    De Corte, Daniele; Sintes, Eva; Yokokawa, Taichi; Reinthaler, Thomas; Herndl, Gerhard J

    2012-08-01

    Viruses are an abundant, diverse and dynamic component of marine ecosystems and have a key role in the biogeochemical processes of the ocean by controlling prokaryotic and phytoplankton abundance and diversity. However, most of the studies on virus-prokaryote interactions in marine environments have been performed in nearshore waters. To assess potential variations in the relation between viruses and prokaryotes in different oceanographic provinces, we determined viral and prokaryotic abundance and production throughout the water column along a latitudinal transect in the North Atlantic. Depth-related trends in prokaryotic and viral abundance (both decreasing by one order of magnitude from epi- to abyssopelagic waters), and prokaryotic production (decreasing by three orders of magnitude) were observed along the latitudinal transect. The virus-to-prokaryote ratio (VPR) increased from ~19 in epipelagic to ~53 in the bathy- and abyssopelagic waters. Although the lytic viral production decreased significantly with depth, the lysogenic viral production did not vary with depth. In bathypelagic waters, pronounced differences in prokaryotic and viral abundance were found among different oceanic provinces with lower leucine incorporation rates and higher VPRs in the North Atlantic Gyre province than in the provinces further north and south. The percentage of lysogeny increased from subpolar regions toward the more oligotrophic lower latitudes. Based on the observed trends over this latitudinal transect, we conclude that the viral-host interactions significantly change among different oceanic provinces in response to changes in the biotic and abiotic variables. PMID:22258100

  20. Urban Transects

    OpenAIRE

    Tixier, Nicolas; Melemis, Steven; Brayer, Laure

    2010-01-01

    International audience Developed within the framework of a Frenchinterdisciplinary research program and a design studioMaster’s program of the Grenoble School ofArchitecture’s entitled Architecture, Cultures of theSenses and the Environment, we have explored thenotion of the “Urban Section” as a meeting-pointbetween global environmental issues and situated,atmospheric qualities of space, as experienced throughspatial practices. This initial idea was to develop thecapacity of the urban sect...

  1. Magnetopause transects

    Science.gov (United States)

    Sonnerup, B. U. Ö.; Guo, M.

    A novel method is described for reconstruction of two-dimensional current-layer structures from measurements taken by a single spacecraft traversing the layer. In its present form, the method is applicable only to 2D magneto-hydrostatic structures that are passively convected past the observing spacecraft. It is tested on a magnetopause crossing of the tangential-discontinuity type by the spacecraft AMPTE/IRM. The magnetic structures recovered include a magnetic island located between two X-type nulls as well as a magnetic ‘worm hole’ through which a bundle of weak magnetic flux appears to connect the magnetosphere and the magnetosheath.

  2. Quantitative analysis of photoquadrats acquired at Johnston Atoll permanent transect site 1A-P on June 29, 2000

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — This spreadsheet summarizes the number of corals photographed along a permanent transect line at Underwater Site 1A-P at Johnston Atoll on 6/29/2000.

  3. FBSAD Recruit Reef Fish Belt Transect Survey at Hawaii Island (Big Island), Main Hawaiian Islands, 2005 (NODC Accession 0046935)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Shore-based belt transects were conducted at 8-13 m depths at 3 longshore sites on the leeward coast (North and South Kohala districts) of the Big Island (Hawaii...

  4. FBSAB RECRUIT Reef Fish Belt Transect Survey at Hawaii Island (Big Island), Main Hawaiian Islands, 2009 (NODC Accession 0073870)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Shore-based belt transects were conducted at 1 to ~ 5 m depths at a total two (2) sites on the leeward coast (South Kohala district) of the Big Island (Hawaii...

  5. Groundwater Dynamics along Forest-Marsh-Tidal Creek Transects in North Inlet Estuary, South Carolina: 1994-1996

    Data.gov (United States)

    Baruch Institute for Marine and Coastal Sciences, Univ of South Carolina — Ground water level elevations were collected every 10 to 15 days from piezometers stationed along three forest-marsh-tidal creek transects (B, C, and D) across the...

  6. Quantitative analysis of photoquadrats acquired at Johnston Atoll permanent transect site 2A-P on June 30, 2000

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — This spreadsheet summarizes the number of corals photographed along a permanent transect line at Underwater Site 2AP at Johnston Atoll on 6302000.

  7. Quantitative analysis of photoquadrats acquired at Johnston Atoll permanent transect site 3A-P on July 1, 2000

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — This spreadsheet summarizes the number of corals photographed along a permanent transect line at Underwater Site 3AP at Johnston Atoll on 712000.

  8. Spatial distribution of phytoplankton in Spring 2004 along a transect in the eastern part of the North Sea

    DEFF Research Database (Denmark)

    Skaloud, P.; Rezacova, M.; Ellegaard, Marianne

    2006-01-01

    , although the species composition was different. Some species were found to prefer coastal waters, other species were characterized as oceanic, and several species were found at all stations. Porosira glacialis showed an atypical distribution along the transect, with highest abundances at both coastal and......We report the results from a 250 km long transect, from the Danish coast to the North Sea at 55°30' N, which was sampled every 32 km in order to study the composition and distribution of phytoplankton, and their dependence on the distance from the coast, depth and other environmental factors...... algae. The other less abundant groups were Dinophyceae, Dictyochophyceae, Prasinophyceae and Chlorophyceae. The pattern of distribution of diatoms and dinophytes along the transect was more or less similar, with larger numbers of cells found close to both the eastern and western parts of the transect...

  9. Traumatic cervical cord transection without facet dislocations--a proposal of combined hyperflexion-hyperextension mechanism: a case report.

    Science.gov (United States)

    Cha, Yoo-Hyun; Cho, Tai-Hyoung; Suh, Jung-Keun

    2010-08-01

    A patient is presented with a cervical spinal cord transection which occurred after a motor vehicle accident in which the air bag deployed and the seat belt was not in use. The patient had complete quadriplegia below the C5 level and his imaging study showed cervical cord transection at the level of the C5/6 disc space with C5, C6 vertebral bodies and laminar fractures. He underwent a C5 laminectomy and a C4-7 posterior fusion with lateral mass screw fixation. Previous reports have described central cord syndromes occurring in hyperextension injuries, but in adults, acute spinal cord transections have only developed after fracture-dislocations of the spine. A case involving a post-traumatic spinal cord transection without any evidence of radiologic facet dislocations is reported. Also, we propose a combined hyperflexion-hyperextension mechanism to explain this type of injury.

  10. Video Transect Images from the Hawaii Coral Reef Assessment and Monitoring Program (CRAMP): Data from 2003 (NODC Accession 0001732)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This dataset consists of video transect images (TIF files) from CRAMP surveys taken in 2003 at 15 sites, some of which had multiple depths. Estimates of substrate...

  11. AFSC/NMML: Cetacean line-transect survey in the eastern Bering Sea shelf; 1999, 2000, 2002, and 2004

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Visual surveys for cetaceans were conducted on the eastern Bering Sea shelf along transect lines, in association with the AFSC.s echo integration trawl surveys for...

  12. Video Transect Images from the Hawaii Coral Reef Assessment and Monitoring Program (CRAMP): Data from 2000 (NODC Accession 0000728)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This dataset consists of video transect images (TIF files) from CRAMP surveys taken in 2000 at 23 sites, some of which had multiple depths. Estimates of substrate...

  13. Video Transect Images from the Hawaii Coral Reef Assessment and Monitoring Program (CRAMP): Data from 2002 (NODC Accession 0000961)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This dataset consists of video transect images (TIF files) from CRAMP surveys taken in 2002 at 23 sites, some of which had multiple depths. Estimates of substrate...

  14. Digital Still Transect Images from the Hawaii Coral Reef Assessment and Monitoring Program (CRAMP): Data from 2004 (NODC Accession 0037908)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This dataset consists of video transect images (JPG files) from CRAMP surveys taken in 2004 at 6 sites, some of which had multiple depths. Digital images are...

  15. Video transect images from the Hawaii Coral Reef Assessment and Monitoring Program (CRAMP): data from year 1999 (NODC Accession 0000671)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This dataset consists of video transect images (JPEG files) from CRAMP surveys taken in 1999 at 26 sites, some of which had multiple depths. Estimates of substrate...

  16. Video Transect Images from the Hawaii Coral Reef Assessment and Monitoring Program (CRAMP):Data from 2003 (NODC Accession 0001732)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This dataset consists of video transect images (TIF files) from CRAMP surveys taken in 2003 at 15 sites, some of which had multiple depths. Estimates of substrate...

  17. Video transect images from the Hawaii Coral Reef Assessment and Monitoring Program (CRAMP): data from 2002 (NODC Accession 0000961)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This dataset consists of video transect images (TIF files) from CRAMP surveys taken in 2002 at 23 sites, some of which had multiple depths. Estimates of substrate...

  18. Video Transect Images (1999) from the Hawaii Coral Reef Assessment and Monitoring Program (CRAMP) (NODC Accession 0000671)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This dataset consists of video transect images (JPEG files) from CRAMP surveys taken in 1999 at 26 sites, some of which had multiple depths. Estimates of substrate...

  19. Digital Still Transect Images from the Hawaii Coral Reef Assessment and Monitoring Program (CRAMP) Maui 2006 (NODC Accession 0039627)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This dataset consists of digital still transect images (JPG files) from CRAMP surveys taken on Maui in 2006 at 8 sites, some of which had multiple depths. Surveys...

  20. Video transect images from the Hawaii Coral Reef Assessment and Monitoring Program (CRAMP): data from 2000 (NODC Accession 0000728)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This dataset consists of video transect images (TIF files) from CRAMP surveys taken in 2000 at 23 sites, some of which had multiple depths. Estimates of substrate...

  1. How much effort should be employed for surveying a low-diversity Amazonian mammal assemblage using line-transects?

    Directory of Open Access Journals (Sweden)

    Ítalo Mourthé

    2013-02-01

    Full Text Available Line-transect surveys are commonly used for sampling large mammals, but estimates of the effort needed to reliably surveying low-diversity assemblages of mammals are scarce. Using data from line-transects and species accumulation curves, I examined whether or not a sampling effort previously suggested to survey mammals elsewhere (ca. 85-100 km would be satisfactory for surveying a low-diversity assemblage of large mammals in the Rio Negro basin in northern Amazonia. In total, 14 mammals were recorded after an accumulated effort of 690 km walked. The desired threshold of completeness was only achieved in one of six transects after an average effort of 115 km surveyed. Considering the entire landscape (all transects pooled, survey completeness was reached after a much higher effort. Moreover, the theoretical effort required to achieve completeness was estimated to be 150-360 km per transect, and 512 km for the landscape. Further studies are required to fully understand this issue, but meanwhile it is safest to assume that higher sampling efforts should be employed when surveying low-diversity assemblages through diurnal line-transects in northwestern Amazonia to get robust estimates of mammal richness.

  2. Morphological and Genetic Variation along a North-to-South Transect in Stipa purpurea, a Dominant Grass on the Qinghai-Tibetan Plateau: Implications for Response to Climate Change.

    Science.gov (United States)

    Liu, Wensheng; Zhao, Yao; You, Jianling; Qi, Danhui; Zhou, Yin; Chen, Jiakuan; Song, Zhiping

    2016-01-01

    Estimating the potential of species to cope with rapid environmental climatic modifications is of vital importance for determining their future viability and conservation. The variation between existing populations along a climatic gradient may predict how a species will respond to future climate change. Stipa purpurea is a dominant grass species in the alpine steppe and meadow of the Qinghai-Tibetan Plateau (QTP). Ecological niche modelling was applied to S. purpurea, and its distribution was found to be most strongly correlated with the annual precipitation and the mean temperature of the warmest quarter. We established a north-to-south transect over 2000 km long on the QTP reflecting the gradients of temperature and precipitation, and then we estimated the morphological by sampling fruited tussocks and genetic divergence by using 11 microsatellite markers between 20 populations along the transect. Reproductive traits (the number of seeds and reproductive shoots), the reproductive-vegetative growth ratio and the length of roots in the S. purpurea populations varied significantly with climate variables. S. purpurea has high genetic diversity (He = 0.585), a large effective population size (Ne >1,000), and a considerable level of gene flow between populations. The S. purpurea populations have a mosaic genetic structure: some distant populations (over 1000 km apart) clustered genetically, whereas closer populations (transect and might be mediated by seed dispersal via migratory herbivores, such as the chiru (Pantholops hodgsonii). These findings suggest that population performance variation and gene flow both facilitate the response of S. purpurea to climate change.

  3. A floristic survey of the Hyrcanian forests in Northern Iran, using two lowland-mountain transects

    Institute of Scientific and Technical Information of China (English)

    Alireza Naqinezhad; Hassan Zare-Maivan; Hamid Gholizadeh

    2015-01-01

    We investigated the floristic composition of the Hyrcanian forests and the related forest-steppe ecotone in Northern Iran by using two long ecological transects, from lowland to upper mountain areas. The study was conducted during 2008 and 2009 and yielded the identification of 395 plant taxa belonging to 233 genera and 78 families. Dicots with 300 taxa were the richest groups of the flora, followed by monocots with 75 taxa, pteridophytes with 18 species, and gymnosperms with two species. The largest families were Asteraceae (33 taxa); Rosaceae (32 taxa); and Poa-ceae (30 taxa), and the most diverse genera included Carex (15 taxa); Alchemilla (7 taxa); and Poa, Geranium and Acer (6 taxa each). Hemicryptophytes were the most dominant life forms in the area (40%); followed by geo-phytes (31.4%); phanerophytes (15.4%); therophytes (11.4%);and chamaephytes (1.8%). Phytogeographically, Euro-Siberian/Irano-Turanian elements (86 taxa, 21.8%) and Euro-Siberian elements (85 taxa, 21.5%) were the most common chorotypes in the area. Out of 395 taxa, 66 taxa (16.7%) were endemics and subendemics in Iran, of which 26 taxa were exclusively endemics of Iran. According to the IUCN Red List Categories, 48 threatened plant taxa were found in the study area. Plant diversity, life form, and chorotypes in the current study were compared with similar transect studies in other areas of the Hyrcanian forests and in different altitudinal belts, using Sørenson similarity indices. Floristic composition of the surveyed transects demonstrated almost 50% similarity between them.

  4. Inferotemporal-frontal disconnection and fornix transection in visuomotor conditional learning by monkeys.

    Science.gov (United States)

    Gaffan, D; Harrison, S

    1988-12-01

    The first 2 experiments examined the ability of monkeys (Macaca fascicularis) to learn a series of visuomotor conditional discrimination problems for food reward. In each discrimination problem there were 2 visual stimuli, which were different from those in any previous discrimination problem. Each trial within a problem presented either one visual stimulus or the other, and the stimulus indicated which of 2 motor responses, tap or hold, was correct; the motor responses were defined in such a way as to be mutually exclusive. Successive problems were each trained to a criterion of correct performance. In the first experiment, it was found that fornix transection did not impair monkey's learning rate in this task. This result contrasts with previous results showing an impairment of learning rate following fornix transection in visuo-motor conditional discriminations in which the 2 motor responses were differentiated from each other by their spatial position or direction. The present result shows that the earlier demonstrated impairments are specific to spatial responses. In the second experiment, learning rate in the present task was found to be impaired by the combination of a unilateral inferotemporal ablation, contralateral to the hand used in the task by the animal, with a transection of the anterior corpus callosum. This result suggests that cortico-cortical interaction between the inferotemporal area and the frontal lobe contralateral to the hand in use is necessary for efficient learning in this task. The third experiment examined simultaneous 2-choice visual discrimination learning in the animals which had participated in experiment 2. In contrast to the results of Expt. 2, learning rate in this task was unimpaired. Experiment 3 shows that the impairment observed in Expt. 2 is not a general impairment of visual learning. PMID:3202946

  5. Minocycline mechanism of neuroprotection involves the Bcl-2 gene family in optic nerve transection.

    Science.gov (United States)

    Levkovitch-Verbin, Hani; Waserzoog, Yael; Vander, Shelly; Makarovsky, Daria; Ilia, Piven

    2014-10-01

    The second-generation tetracycline, minocycline, has been shown to exhibit neuroprotective therapeutic benefits in many neurodegenerative diseases including experimental glaucoma and optic nerve transection (ONT). This study investigated the mechanism underlying minocycline neuroprotection in a model of ONT. ONT was applied unilaterally in 36 Wistar rat eyes. The rats were randomly divided into a minocycline (22 mg/kg/d) treatment group and a saline treatment group (control). Treatment (minocycline or saline) was given by intraperitoneal injections initiated 3 d before ONT and continued daily until the end of the experiment. The involvement of pro-apoptotic, pro-survival and inflammatory pathways was analyzed by quantitative Real-Time Polymerase Chain Reaction at 4 h and 3 d after the transection in both treatment groups. The involvement of Bcl-2 protein was evaluated by immunohistochemistry. We found that Minocycline significantly increased the expression of the antiapoptotic gene bcl-2 4 h after transection (n = 8, p = 0.008) and decreased the expression of Bax at the same time point (n = 8, p = 0.03). Tumor Necrosis Factor α (TNFα), Inhibitor of Apoptosis Protein (IAP1) and Gadd45α were significantly upregulated in the retinas of eyes with ONTs compared to control (n = 10 for each gene, p = 0.02, p = 0.03, p = 0.04, respectively) but this effect was unaffected by minocycline. This study further support that the mechanism underlying minocycline neuroprotection involves the Bcl-2 gene family, suggesting that minocycline has antiapoptotic properties that support its value as a promising neuroprotective drug. PMID:24410139

  6. Combined Effect of Bilateral Ovariectomy and Anterior Cruciate Ligament Transection With Medial Meniscectomy on the Development of Osteoarthritis Model

    Science.gov (United States)

    2016-01-01

    Objective To investigate the combined effect of bilateral ovariectomy (OVX) and anterior cruciate ligament transection (ACLT) with medial meniscectomy (MM) on the development of osteoarthritis (OA). Methods Twenty female 15-week-old Sprague-Dawley rats were used. Five rats in each group underwent bilateral OVX (OVX group), bilateral ACLT with MM (ACLT with MM group), bilateral OVX plus ACLT with MM (OVX plus ACLT with MM group), and sham surgery (SHAM group). All the rats were subjected to treadmill running for 4 weeks. The behavioral evaluation for induction of OA used the number of rears method, and this was conducted at 1, 2, and 4 weeks post-surgery. Bone mineral density (BMD) was calculated with micro-computerized tomography images and the modified Mankin's scoring was used for the histological changes. Results The number of rears in the OVX plus ACLT with MM group decreased gradually and more rapidly in the ACLT with MM group. Histologically, the OVX plus ACLT with MM group had a significantly higher modified Mankin's score than the OVX group (p=0.008) and the SHAM group (p=0.008). BMDs of the OVX plus ACLT with MM group were significantly lower than the SHAM group (p=0.002), and the ACLT with MM group (p=0.003). Conclusion We found that bilateral OVX plus ACLT with MM induced definite OA change in terms of histology and BMD compared to bilateral OVX and ACLT with MM alone. Therefore, OVX and ACLT with MM was an appropriate degenerative OA rat model. PMID:27606264

  7. Panax notoginseng saponins improve recovery after spinal cord transection by upregulating neurotrophic factors

    Institute of Scientific and Technical Information of China (English)

    Bo Wang; Yu Li; Xuan-peng Li; Yang Li

    2015-01-01

    Saponins extracted fromPanax notoginseng are neuroprotective, but the mechanisms underlying this effect remain unclear. In the present study, we established a rat model of thoracic (T10) spinal cord transection, and injectedPanax notoginseng saponins (100 mg/kg) or saline 30 minutes after injury. Locomotor functions were assessed using the Basso, Beattie, and Bresnahan (BBB) scale from 1 to 30 days after injury, and immunohistochemistry was carried out in the ventral horn of the spinal cord at 1 and 7 days to determine expression of nerve growth factor (NGF) and brain-derived neurotrophic factor (BDNF). Our results show that at 7–30 days post injury, the BBB score was higher in rats treated withPanax notoginseng saponins than in those that received saline. Furthermore, at 7 days, more NGF- and BDNF-immunoreactive neurons were observed in the ventral horn of the spinal cord of rats that had receivedPanax notoginseng saponins than in those that received saline. These results indicate thatPanax notoginseng saponins caused an upregulation of NGF and BDNF in rats with spinal cord transection, and improved hindlimb motor function.

  8. Gravity Transects across the Karonga Fault in the Northern Malawi Rift

    Science.gov (United States)

    Hull, C. D.; Johnson, T.; Chindandali, P. R. N.; Clappe, B.; Dawson, S.; Laó-Dávila, D. A.; Atekwana, E. A.; Abdelsalam, M. G.; Ivey, T.; Nyalugwe, V.; Salima, J.

    2015-12-01

    The Karonga area of northern Malawi is in the hanging wall of the Livingstone border fault. While most of the strain is accommodated along the border fault, recent seismicity and remote sensing studies indicate there is strain accommodation in the hanging wall area. Gravity data were collected along two West-East transects (one in the north and the other in the south) in this region to explore this strain accommodation, locate faults, determine the thickness of sediments on the hanging wall block of the Karonga fault, and to determine the geometry of a Karoo depositional basin. Both profiles extended from the uplifted basements into the rift basin; the northern profile is approximately 30 km in length while the southern profile is 70 km. Both gravity profiles were acquired with a Scintrex CG-5 Autograv using station spacings ranging between 0.5 - 2 km. These gravity transects place constraints on the structural interpretations of the area based on previous fieldwork and remote sensing. This project uses 2.5D forward gravity modeling to test the possibility of the Karonga fault being a single fault in the north while breaking up into multiple faults further south as it responds to the weaknesses created by preexisting structures.

  9. Harmonic scalpel for a bloodless partial glossectomy: a case report.

    Science.gov (United States)

    Irfan, M; Aliyu, Y A; Baharudin, A; Shahid, H

    2011-06-01

    Tongue surgery is almost always complicated by intraoperative bleeding. Its rich blood supply especially from the lingual vessels makes the operative field bloody. Electrocautery has been widely used to replace cold scissors in order to achieve better hemostasis. The use of ultrasonic harmonic scalpel for glossectomy is still new in this country. We report a case of partial glossectomy using the harmonic scalpel in a patient who had a squamous cell carcinoma of the lateral border of the tongue.

  10. Cross-continental comparison of the functional composition and carbon allocation of two altitudinal forest transects in Ecuador and Rwanda.

    Science.gov (United States)

    Bauters, Marijn; Bruneel, Stijn; Demol, Miro; Taveirne, Cys; Van Der Heyden, Dries; Boeckx, Pascal; Kearsley, Elizabeth; Cizungu, Landry; Verbeeck, Hans

    2016-04-01

    Tropical forests are key actors in the global carbon cycle. Predicting future responses of these forests to global change is challenging, but important for global climate models. However, our current understanding of such responses is limited, due to the complexity of forest ecosystems and the slow dynamics that inherently form these systems. Our understanding of ecosystem ecology and functioning could greatly benefit from experimental setups including strong environmental gradients in the tropics, as found on altitudinal transects. We setup two such transects in both South-America and Africa, focussing on shifts in carbon allocation, forest structure and functional composition. By a cross-continental comparison of both transects, we will gain insight in how different or alike both tropical forests biomes are in their responses, and how universal the observed adaption mechanisms are.

  11. Expanding biological data standards development processes for US IOOS: visual line transect observing community for mammal, bird, and turtle data

    Science.gov (United States)

    Fornwall, M.; Gisiner, R.; Simmons, S. E.; Moustahfid, Hassan; Canonico, G.; Halpin, P.; Goldstein, P.; Fitch, R.; Weise, M.; Cyr, N.; Palka, D.; Price, J.; Collins, D.

    2012-01-01

    The US Integrated Ocean Observing System (IOOS) has recently adopted standards for biological core variables in collaboration with the US Geological Survey/Ocean Biogeographic Information System (USGS/OBIS-USA) and other federal and non-federal partners. In this Community White Paper (CWP) we provide a process to bring into IOOS a rich new source of biological observing data, visual line transect surveys, and to establish quality data standards for visual line transect observations, an important source of at-sea bird, turtle and marine mammal observation data. The processes developed through this exercise will be useful for other similar biogeographic observing efforts, such as passive acoustic point and line transect observations, tagged animal data, and mark-recapture (photo-identification) methods. Furthermore, we suggest that the processes developed through this exercise will serve as a catalyst for broadening involvement by the larger marine biological data community within the goals and processes of IOOS.

  12. Involvement of medullary GABAergic system in extraterritorial neuropathic pain mechanisms associated with inferior alveolar nerve transection.

    Science.gov (United States)

    Okada-Ogawa, Akiko; Nakaya, Yuka; Imamura, Yoshiki; Kobayashi, Masayuki; Shinoda, Masamichi; Kita, Kozue; Sessle, Barry J; Iwata, Koichi

    2015-05-01

    In order to determine if the functional changes in the GABAergic system in the trigeminal spinal subnucleus caudalis (Vc) are involved in the mechanisms underlying extraterritorial neuropathic pain in the orofacial region following inferior alveolar nerve transection (IANX), mechanical noxious behavior, phosphorylated extracellular signal-regulated kinase (pERK) immunohistochemistry and single neuronal activity were analyzed in vesicular GABA transporter (VGAT)-VenusA rats expressing fluorescent protein and the VGAT in Vc neurons. The number of VGAT-VenusA positive neurons was significantly reduced in IANX rats than naive and sham rats at 7days after nerve transection. The number of VGAT-VenusA positive pERK-immunoreactive (IR) cells was significantly increased in IANX rats at 21days after IAN transection compared with naive and sham rats. The background activity and mechanical-evoked responses of Vc nociceptive neurons were significantly depressed after intrathecal application of the GABA receptor agonist muscimol in sham rats but not in IANX rats. Furthermore, the expression of potassium-chloride co-transporter 2 (KCC2) in the Vc was significantly reduced in IANX rats compared with sham rats. The head-withdrawal threshold (HWT) to mechanical stimulation of the whisker pad skin was significantly decreased in IANX rats compared with sham rats on days 7 and 21 after IANX. The significant reduction of the HWT and significant increase in the number of VGAT-VenusA negative pERK-IR cells were observed in KCC2 blocker R-DIOA-injected rats compared with vehicle-injected rats on day 21 after sham treatment. These findings revealed that GABAergic Vc neurons might be reduced in their number at the early period after IANX and the functional changes might occur in GABAergic neurons from inhibitory to excitatory at the late period after IANX, suggesting that the neuroplastic changes occur in the GABAergic neuronal network in the Vc due to morphological and functional changes at

  13. Line Transect Surveys Underdetect Terrestrial Mammals: Implications for the Sustainability of Subsistence Hunting.

    Science.gov (United States)

    Fragoso, José M V; Levi, Taal; Oliveira, Luiz F B; Luzar, Jeffrey B; Overman, Han; Read, Jane M; Silvius, Kirsten M

    2016-01-01

    Conservation of Neotropical game species must take into account the livelihood and food security needs of local human populations. Hunting management decisions should therefore rely on abundance and distribution data that are as representative as possible of true population sizes and dynamics. We simultaneously applied a commonly used encounter-based method and an infrequently used sign-based method to estimate hunted vertebrate abundance in a 48,000-km2 indigenous landscape in southern Guyana. Diurnal direct encounter data collected during three years along 216, four-kilometer -long transects consistently under-detected many diurnal and nocturnal mammal species readily detected through sign. Of 32 species analyzed, 31 were detected by both methods; however, encounters did not detect one and under-detected another 12 of the most heavily hunted species relative to sign, while sign under-detected 12 never or rarely collected species relative to encounters. The six most important game animals in the region, all ungulates, were not encountered at 11-40% of village and control sites or on 29-72% of transects where they were detected by sign. Using the sign methodology, we find that tapirs, one of the terrestrial vertebrates considered most sensitive to overexploitation, are present at many sites where they were never visually detected during distance sampling. We find that this is true for many other species as well. These high rates of under-detection suggest that behavioral changes in hunted populations may affect apparent occurrence and abundance of these populations. Accumulation curves (detection of species on transects) were much steeper for sign for 12 of 16 hunted species than for encounters, but that pattern was reversed for 12 of 16 species unhunted in our area. We conclude that collection of sign data is an efficient and effective method of monitoring hunted vertebrate populations that complements encounter and camera-trapping methods in areas impacted by

  14. Contrasting pH buffering patterns in neutral-alkaline soils along a 3600 km transect in northern China

    Directory of Open Access Journals (Sweden)

    W. Luo

    2015-08-01

    Full Text Available Soil pH buffering capacity (pHBC plays a crucial role in predicting acidification rates, yet its large-scale patterns and controls are poorly understood, especially for neutral-alkaline soils. Here, we evaluated the spatial patterns and drivers of pHBC along a 3600 km long transect (1900 km sub-transect with carbonate containing soils and 1700 km sub-transect with non-carbonate containing soils across northern China. Soil pHBC was greater in the carbonate containing soils than in the non-carbonate containing soils. Acid addition decreased soil pH in the non-carbonate containing soils more markedly than in the carbonate containing soils. Within the carbonate soil sub-transect, soil pHBC was positively correlated with cation exchange capacity (CEC, carbonate content and exchangeable sodium (Na concentration, but negatively correlated with initial pH and clay content, and not correlated with soil organic carbon (SOC content. Within the non-carbonate sub-transect, soil pHBC was positively related to initial pH, clay content, CEC and exchangeable Na concentration, but not related to SOC content. Carbonate content was the primary determinant of pHBC in the carbonate containing soils and CEC was the main determinant of buffering capacity in the non-carbonate containing soils. Soil pHBC was positively related to aridity index and carbonate content across the carbonate containing soil sub-transect. Our results indicated that mechanisms controlling pHBC differ among neutral-alkaline soils of northern China, especially between carbonate and non-carbonate containing soils, leading to different rates, risks, and impacts of acidification. This understanding should be incorporated into the acidification risk assessment and landscape management in a changing world.

  15. Traumatic Cervical Cord Transection without Facet Dislocations-A Proposal of Combined Hyperflexion-Hyperextension Mechanism: A Case Report

    OpenAIRE

    Cha, Yoo-Hyun; Cho, Tai-Hyoung; Suh, Jung-Keun

    2010-01-01

    A patient is presented with a cervical spinal cord transection which occurred after a motor vehicle accident in which the air bag deployed and the seat belt was not in use. The patient had complete quadriplegia below the C5 level and his imaging study showed cervical cord transection at the level of the C5/6 disc space with C5, C6 vertebral bodies and laminar fractures. He underwent a C5 laminectomy and a C4-7 posterior fusion with lateral mass screw fixation. Previous reports have described ...

  16. Accounting for subgroup structure in line-transect abundance estimates of false killer whales (Pseudorca crassidens in Hawaiian waters.

    Directory of Open Access Journals (Sweden)

    Amanda L Bradford

    Full Text Available For biological populations that form aggregations (or clusters of individuals, cluster size is an important parameter in line-transect abundance estimation and should be accurately measured. Cluster size in cetaceans has traditionally been represented as the total number of individuals in a group, but group size may be underestimated if group members are spatially diffuse. Groups of false killer whales (Pseudorca crassidens can comprise numerous subgroups that are dispersed over tens of kilometers, leading to a spatial mismatch between a detected group and the theoretical framework of line-transect analysis. Three stocks of false killer whales are found within the U.S. Exclusive Economic Zone of the Hawaiian Islands (Hawaiian EEZ: an insular main Hawaiian Islands stock, a pelagic stock, and a Northwestern Hawaiian Islands (NWHI stock. A ship-based line-transect survey of the Hawaiian EEZ was conducted in the summer and fall of 2010, resulting in six systematic-effort visual sightings of pelagic (n = 5 and NWHI (n = 1 false killer whale groups. The maximum number and spatial extent of subgroups per sighting was 18 subgroups and 35 km, respectively. These sightings were combined with data from similar previous surveys and analyzed within the conventional line-transect estimation framework. The detection function, mean cluster size, and encounter rate were estimated separately to appropriately incorporate data collected using different methods. Unlike previous line-transect analyses of cetaceans, subgroups were treated as the analytical cluster instead of groups because subgroups better conform to the specifications of line-transect theory. Bootstrap values (n = 5,000 of the line-transect parameters were randomly combined to estimate the variance of stock-specific abundance estimates. Hawai'i pelagic and NWHI false killer whales were estimated to number 1,552 (CV = 0.66; 95% CI = 479-5,030 and 552 (CV = 1.09; 95% CI = 97

  17. Accounting for subgroup structure in line-transect abundance estimates of false killer whales (Pseudorca crassidens) in Hawaiian waters.

    Science.gov (United States)

    Bradford, Amanda L; Forney, Karin A; Oleson, Erin M; Barlow, Jay

    2014-01-01

    For biological populations that form aggregations (or clusters) of individuals, cluster size is an important parameter in line-transect abundance estimation and should be accurately measured. Cluster size in cetaceans has traditionally been represented as the total number of individuals in a group, but group size may be underestimated if group members are spatially diffuse. Groups of false killer whales (Pseudorca crassidens) can comprise numerous subgroups that are dispersed over tens of kilometers, leading to a spatial mismatch between a detected group and the theoretical framework of line-transect analysis. Three stocks of false killer whales are found within the U.S. Exclusive Economic Zone of the Hawaiian Islands (Hawaiian EEZ): an insular main Hawaiian Islands stock, a pelagic stock, and a Northwestern Hawaiian Islands (NWHI) stock. A ship-based line-transect survey of the Hawaiian EEZ was conducted in the summer and fall of 2010, resulting in six systematic-effort visual sightings of pelagic (n = 5) and NWHI (n = 1) false killer whale groups. The maximum number and spatial extent of subgroups per sighting was 18 subgroups and 35 km, respectively. These sightings were combined with data from similar previous surveys and analyzed within the conventional line-transect estimation framework. The detection function, mean cluster size, and encounter rate were estimated separately to appropriately incorporate data collected using different methods. Unlike previous line-transect analyses of cetaceans, subgroups were treated as the analytical cluster instead of groups because subgroups better conform to the specifications of line-transect theory. Bootstrap values (n = 5,000) of the line-transect parameters were randomly combined to estimate the variance of stock-specific abundance estimates. Hawai'i pelagic and NWHI false killer whales were estimated to number 1,552 (CV = 0.66; 95% CI = 479-5,030) and 552 (CV = 1.09; 95% CI = 97

  18. Vegetation biomass, leaf area index, and NDVI patterns and relationships along two latitudinal transects in arctic tundra

    Science.gov (United States)

    Epstein, H. E.; Walker, D. A.; Raynolds, M. K.; Kelley, A. M.; Jia, G.; Ping, C.; Michaelson, G.; Leibman, M. O.; Kaarlejärvi, E.; Khomutov, A.; Kuss, P.; Moskalenko, N.; Orekhov, P.; Matyshak, G.; Forbes, B. C.; Yu, Q.

    2009-12-01

    Analyses of vegetation properties along climatic gradients provide first order approximations as to how vegetation might respond to a temporally dynamic climate. Until recently, no systematic study of tundra vegetation had been conducted along bioclimatic transects that represent the full latitudinal extent of the arctic tundra biome. Since 1999, we have been collecting data on arctic tundra vegetation and soil properties along two such transects, the North American Arctic Transect (NAAT) and the Yamal Arctic Transect (YAT). The NAAT spans the arctic tundra from the Low Arctic of the North Slope of Alaska to the polar desert of Cape Isachsen on Ellef Ringnes Island in the Canadian Archipelago. The Yamal Arctic Transect located in northwest Siberia, Russia, presently ranges from the forest-tundra transition at Nadym to the High Arctic tundra on Belyy Ostrov off the north coast of the Yamal Peninsula. The summer warmth indices (SWI - sum of mean monthly temperatures greater than 0°C) range from approximately 40 °C months to 3 °C months from south to north. For largely zonal sites along these transects, we systematically collected leaf area index (LAI-2000 Plant Canopy Analyzer), normalized difference vegetation index (NDVI - PSII hand-held spectro-radiometer), and vegetation biomass (clip harvests). Site-averaged LAI ranges from 1.08 to 0 along the transects, yet can be highly variable at the landscape scale. Site-averaged NDVI ranges from 0.67 to 0.26 along the transects, and is less variable than LAI at the landscape scale. Total aboveground live biomass ranges from approximately 700 g m-2 to < 50 g m-2 along the NAAT, and from approximately 1100 g m-2 to < 400 g m-2 along the YAT (not including tree biomass at Nadym). LAI and NDVI are highly correlated logarithmically (r = 0.80) for the entire dataset. LAI is significantly related to total aboveground (live plus dead) vascular plant biomass, although there is some variability in the data (r = 0.63). NDVI is

  19. Establishment and evaluation of a rat model of complete transected spinal cord injury

    Institute of Scientific and Technical Information of China (English)

    Xuejun Li; Chunhai Huang; Shangming Liu; Xianrui Yuan

    2008-01-01

    BACKGROUND: The establishment of a rat model of complete transected spinal cord injury lacks technological specifications. The current models lack concordance and reliability, and the death rate of the experimental animals is high. Therefore, there is a great need for a reliable model to apply clinical applications of therapy.OBJECTIVE: To construct a rat model of complete transected spinal cord injury characterized by stability, reproducibility, and a high animal survival rate. DESIGN: Completely randomized controlled study.SETTING: Department of Neurosurgery, Xiangya Hospital of Central South University.MATERIALS: Fifty-five healthy specific pathogen free grade adult female Sprague Dawley rats were provided by the Experimental Animal Department, Xiangya Medical College, Central South University. Olympus BX51 imaging collecting analytic system was provided by Olympus Company, Japan; and SEN-7203 Nihon-Kohden electrical stimulator by Nihon Kohden, Japan. METHODS: This study was performed at the Laboratory of Neurosurgery, Xiangya Hospital of Central South University from April to June 2006. Experimental grouping: 55 rats were randomly divided into model group (n = 40) and sham surgery group (n = 15). In the model group, a self-made sliver hook was passed through the ventral side to support the spinal cord at the T12 segment and to shear it off. A complete transected spinal cord, 2 mm in length, was resected. In the sham surgery group, the spinal cord was identically exposed. The dura mater of the spinal cord was cut open, but the spinal cord was not damaged. MAIN OUTCOME MEASURES: Histopathological changes after spinal cord injury at L2 segment were observed subsequent to hematoxylin and eosin staining under optical microscopy. Olympus BX51 imaging collecting analytic system was used to count spinal cord ventral horn neurons. Motor function of rat hindlimb was evaluated with the Basso, Beattie and Bresnahan (BBB) scale. Paraplegia was evaluated as 0 point, and

  20. Jejunal tackle: a case report of complete jejunal transection in rugby union.

    Science.gov (United States)

    Perry, William; Fischer, Jesse; Wakeman, Christopher

    2014-09-01

    Jejunal perforation as a result of blunt abdominal trauma during sport is particularly rare. We are aware of 6 reported cases of jejunal perforation in sport: 1 in hockey, 2 in football (soccer), and 3 in American football. This report presents the case of a 25-year-old professional rugby union player, who presented to an "After Hours" general practice clinic with increasing central abdominal and epigastric pain after a heavy tackle during an international match in New Zealand. Despite suffering complete jejunal transection, the patient continued to play on, only presenting to an After Hours general practice clinic 3 hours after the injury. The case demonstrates the remarkable physiological resilience of professional rugby players and acts as a reminder to maintain a high degree of suspicion for small bowel injury despite normal vital signs in healthy young patients with abdominal pain secondary to blunt trauma.

  1. The lithosphere of Ellesmere Island and adjacent northwestern Greenland (CALE “A” transect onshore)

    DEFF Research Database (Denmark)

    Stephenson, Randell Alexander; Schiffer, Christian; Oakey, Gordon

    orogenesis in the Cenozoic (Eurekan Orogeny). The present-day topography of Ellesmere Island is closely related to the crustal architecture of these tectonic provinces, which includes the adjacent polar continental margin. A two-dimensional lithosphere-scale model is presented that crosses Ellesmere Island...... from northern Baffin Bay to the Arctic Ocean, part of Transect “A” of the Circum-Arctic Lithosphere Evolution (CALE) project. The model is based on gravity and magnetic data constrained by mapped geological structure as well as a not yet unpublished Receiver Function study, based on teleseismic data...... to the lithosphere model. The most recent tectonic event governing the geological and physiographic character of Ellesmere Island is the Eurekan Orogeny, an intraplate orogeny that developed as a consequence of North Atlantic-Arctic plate reorganisations and the resulting convergence of Greenland against Ellesmere...

  2. Missed diagnosis of complete urethral transection after sling: the case for translabial ultrasound.

    Science.gov (United States)

    Rogo-Gupta, Lisa; Le, Ngoc-Bich; Raz, Shlomo

    2012-01-01

    Patients with complications of urethral sling placement for stress urinary incontinence are often treated for recurrent symptoms for years after initial reassuring evaluation. Translabial ultrasound is a noninvasive modality with minimal risks that can clearly diagnose urethral mesh complications. We present a 47-year-old premenopausal woman referred for treatment of urethral stricture and diverticulum 8 years after mesh sling placement. The diagnosis was made at an outside institution by voiding cystourethrogram and cystoscopy. However, translabial ultrasound confirmed the diagnosis of complete urethral transection, and the patient underwent a complex urethral reconstruction. Ultrasound should be used to evaluate patients with a history of urethral sling and persistent lower urinary tract symptoms. Referral to a center with advanced pelvic reconstruction services may be required. PMID:22453271

  3. Temperature and precipitation effects on wheat yield across a European transect

    DEFF Research Database (Denmark)

    Pirttioja, N; Carter, T.; Fronzek, S;

    2015-01-01

    his study explored the utility of the impact response surface (IRS) approach for investigating model ensemble crop yield responses under a large range of changes in climate. IRSs of spring and winter wheat Triticum aestivum yields were constructed from a 26-member ensemble of process-based crop...... simulation models for sites in Finland, Germany and Spain across a latitudinal transect. The sensitivity of modelled yield to systematic increments of changes in temperature (-2 to +9°C) and precipitation (-50 to +50%) was tested by modifying values of baseline (1981 to 2010) daily weather, with CO2...... concentration fixed at 360 ppm. The IRS approach offers an effective method of portraying model behaviour under changing climate as well as advantages for analysing, comparing and presenting results from multi-model ensemble simulations. Though individual model behaviour occasionally departed markedly from...

  4. Line transect estimates of Irrawaddy dolphin abundance along the eastern Gulf Coast of Thailand.

    Directory of Open Access Journals (Sweden)

    Ellen eHines

    2015-09-01

    Full Text Available Effective conservation of coastal marine mammals is largely dependent on reliable knowledge of their abundance, as well as the ecological and human factors driving their distribution. In developing countries, lack of resources and capacity frequently impedes research needed to estimate abundance and to determine the ecological requirements of coastal marine mammals and the impact of threats related to coastal development and fisheries. Over the course of five years, we developed practical research methods and trained local scientists in Thailand to use accepted line transect distance sampling methods for abundance assessment. The study focused on a little-known coastal and freshwater species found throughout Southeast Asia, namely the Irrawaddy dolphin, which has been sighted regularly along the coast of the eastern Gulf of Thailand. During five years of line transect boat surveys in Trat Province, the eastern-most province in Thailand, we found an average of 423 dolphins distributed within 12km of the coast. Compared to other abundance estimates of coastal Irrawaddy dolphins in Southeast Asia, this is a relatively large number. This population could extend into the northern coast of Cambodia, where surveys are currently being planned. The Thai government has begun talks with Cambodia about a transboundary marine protected area that would include areas in both countries where coastal Irrawaddy dolphins are found. Other analyses include photo-identification, modeling environmental factors that determine presence, determination of fresh vs. salt water foraging using stable isotopes, and an assessment of threats. Collaboration between scientists in Thailand, Cambodia and Vietnam is further needed to determine dolphin movement and habitat use across borders.

  5. Splenic autotransplantation and oesophageal transection anastomosis in patients with portal hypertension(26 years clinical observation)

    Institute of Scientific and Technical Information of China (English)

    CHEN Jisheng; HUO Jinshan; ZHANG Hongwei; SHANG Changzhen; CHEN Rufu; ZHANG Jie; Obetien Mapudengo; CHEN Yajin; ZHANG Lei

    2007-01-01

    The surgical treatment methods for cirrhosis patients complicated with portal hypertension are complicated.In this study,we evaluated the effectiveness of a new treatment strategy:splenic auto-transplantation and oesophageal transection anastomosis on 274 patients from three aspects:clinical observation,splenic immunology and portal dynamics.From 1979 to 2005,274 cirrhosis patients with portal hypertension who underwent the new treatment strategy were followed up to observe different clinical indexes,which were then compared with those of the traditional surgery treatment.From 1999 to 2002,a randomized control trial (RCT) was performed on 40 patients to compare their immune function after operation.From 1994 to 2004,another RCT.was carried out on 28 patients to compare the portal dynamics through three-dimensional dynamic contrast enhanced MR angiography (3D DEC MR.A) investigation after operation.Among 274 patients (mean age 41.8 years),the emergency operative mortality (4.4%),selective operative mortality (2.2%),complication rate (17.9%),morbidity of hepatic encephalopathy ( < 1%),bleeding rate of portal hypertension gastritis (PHG) (9.1%),and morbidity of hepatic carcinoma (8%) were similar to those under traditional operation;the spleen immunology function (Tuftsin,IgM)decreased among the groups 2 months after operation.Through 3D DCE MRA,the cross section area,the velocity and volume of blood flow of main portal vein decrease significantly after operation in both groups,the auto transplantation group was significantly lower in velocity and volume of blood flow than in the control group.Splenic auto transplantation and esophageal transection anastomosis are a safe,effective,and reasonable treatment strategy for portal hypertension with varicial bleeding.It can not only correct hypersplenism but also completely stanch blood,and auto transplanted spleen in the retroperitoneal space can preserve immune function and establish abroad collateral circulation.

  6. Lithospheric conductivity along the GGT/SVEKA transect in the Fennoscandian shield

    Science.gov (United States)

    Lahti, I.; Korja, T.; Petersen, L. B.

    2003-04-01

    LITHOSPHERIC CONDUCTIVITY ALONG THE GGT/SVEKA TRANSECT IN THE FENNOSCANDIAN SHIELD I. Lahti (1), T. Korja (1), L. Pedersen (2) and BEAR Working Group (1) Department of Geophysics, University of Oulu, Finland (2) Department of Earth Sciences, University of Uppsala, Sweden toivo.korja@oulu.fi The GGT/SVEKA transect traverses the main tectonic units in the central part of the Fennoscandian Shield in NE-SW direction. These units are the Archaean Karelian Province in the northeast and several Palaeoproterozoic arc complexes in the Svecofennian Domain in the southwest. Since 1985 over 150 magnetotelluric (MT) soundings of which 140 are short period and 10 long period soundings have been made in the survey area. We have performed several 2-D Occam inversions of the MT data using the REBOCC code (Siripunvaraporn and Egbert, 2000) to generate smooth conductivity models for the survey area. The best fitting model with the RMS error below 3.0% is obtained by using the determinant of impedance tensor as the inverted parameter. Highly conductive dipping conductors at both sides of the boundary zone between the arc complexes in southern and central Finland are seen in the final model. Both conductors represent borders of major crustal segments possibly indicating two subductions in the research area. In contrast, only minor conductivity variations are seen at the lithological boundary between the Karelian and Svecofennian domains in central Finland whereas a southwestward dipping conductor beneath the Palaeoproterozoic Kainuu Belt is revealed. The conductor suggests the presence of Palaeoproterozoic graphite bearing sedimentary rocks beneath the Archaean rocks of the Iisalmi complex. Lower crustal conductor is absent to NE from the Kainuu Belt while the conductor is present in the Palaeoproterozoic Svecofennian Domain to southwest from the Kainuu Belt. Thus, the main conductivity boundary between the Archaean and Palaeoproterozoic lithosphere is located beneath the Kainuu Belt

  7. Global coverage of cetacean line-transect surveys: status quo, data gaps and future challenges.

    Directory of Open Access Journals (Sweden)

    Kristin Kaschner

    Full Text Available Knowledge of abundance, trends and distribution of cetacean populations is needed to inform marine conservation efforts, ecosystem models and spatial planning. We compiled a geo-spatial database of published data on cetacean abundance from dedicated visual line-transect surveys and encoded >1100 abundance estimates for 47 species from 430 surveys conducted worldwide from 1975-2005. Our subsequent analyses revealed large spatial, temporal and taxonomic variability and gaps in survey coverage. With the exception of Antarctic waters, survey coverage was biased toward the northern hemisphere, especially US and northern European waters. Overall, <25% of the world's ocean surface was surveyed and only 6% had been covered frequently enough (≥ 5 times to allow trend estimation. Almost half the global survey effort, defined as total area (km(2 covered by all survey study areas across time, was concentrated in the Eastern Tropical Pacific (ETP. Neither the number of surveys conducted nor the survey effort had increased in recent years. Across species, an average of 10% of a species' predicted range had been covered by at least one survey, but there was considerable variation among species. With the exception of three delphinid species, <1% of all species' ranges had been covered frequently enough for trend analysis. Sperm whales emerged from our analyses as a relatively data-rich species. This is a notoriously difficult species to survey visually, and we use this as an example to illustrate the challenges of using available data from line-transect surveys for the detection of trends or for spatial planning. We propose field and analytical methods to fill in data gaps to improve cetacean conservation efforts.

  8. Mercury and Methylmercury Distributions Along a Longitudinal Transect of the North Atlantic Ocean

    Science.gov (United States)

    Krabbenhoft, D. P.; Maglio, M.; Ogorek, J.; Landing, W. M.; Morton, P. L.; Shelley, R.; Sunderland, E. M.

    2014-12-01

    From August 1 to September 23, 2013 the A16N Repeat Hydrography Cruise (US CLIVAR program) conducted a nearly longitudinal (~20-25°W) sampling transect of the Atlantic Ocean (63°N to 6°S) originating in Reykjavik, Iceland. Water column profiles were taken at 145 locations along this transect, and at 13 of these locations samples for total mercury (THg) and methylmercury (MeHg) were also collected. Although profile depths for this cruise reached nearly 6000 m, Hg and MeHg sampling was limited to 12 discrete samples ranging from the surface to about the 1000 m. All samples were collected while observing strict adherence to trace-metal-free protocols. Upon collection from the rosette, each whole-water sample was immediately acidified to 1% HCl, thus our analysis of THg and MeHg includes both particulate and filter-passing fractions. For MeHg, our results represent a composite of both monomethyl and dimethyl mercury species. All samples were analyzed at the USGS Mercury Research Lab. Our results show several clear and interesting trends. First, an examination of THg versus MeHg reveals a bimodal distribution. Samples with THg > 0.03 pM show a highly correlated and linear distribution (R2=0.72) and a high mean percentage of MeHg (49%). On the other hand, samples with THg levels observed in profiles collected between 9 and 14°N. This region of the North Atlantic Ocean is associated with an oxygen minimum and PCO2 maximum zone both driven by degradation of sinking algal-derived organic matter. Last, the overall results from the Atlantic Ocean show a clear MeHg enrichment compared to similar data collected in the Pacific and Southern Indian Oceans.

  9. Trans-Alaska Crustal Transect and continental evolution involving subduction underplating and synchronous foreland thrusting

    Science.gov (United States)

    Fuis, G.S.; Moore, T.E.; Plafker, G.; Brocher, T.M.; Fisher, M.A.; Mooney, W.D.; Nokleberg, W.J.; Page, R.A.; Beaudoin, B.C.; Christensen, N.I.; Levander, A.R.; Lutter, W.J.; Saltus, R.W.; Ruppert, N.A.

    2008-01-01

    We investigate the crustal structure and tectonic evolution of the North American continent in Alaska, where the continent has grown through magmatism, accretion, and tectonic underplating. In the 1980s and early 1990s, we conducted a geological and geophysical investigation, known as the Trans-Alaska Crustal Transect (TACT), along a 1350-km-long corridor from the Aleutian Trench to the Arctic coast. The most distinctive crustal structures and the deepest Moho along the transect are located near the Pacific and Arctic margins. Near the Pacific margin, we infer a stack of tectonically underplated oceanic layers interpreted as remnants of the extinct Kula (or Resurrection) plate. Continental Moho just north of this underplated stack is more than 55 km deep. Near the Arctic margin, the Brooks Range is underlain by large-scale duplex structures that overlie a tectonic wedge of North Slope crust and mantle. There, the Moho has been depressed to nearly 50 km depth. In contrast, the Moho of central Alaska is on average 32 km deep. In the Paleogene, tectonic underplating of Kula (or Resurrection) plate fragments overlapped in time with duplexing in the Brooks Range. Possible tectonic models linking these two regions include flat-slab subduction and an orogenic-float model. In the Neogene, the tectonics of the accreting Yakutat terrane have differed across a newly interpreted tear in the subducting Pacific oceanic lithosphere. East of the tear, Pacific oceanic lithosphere subducts steeply and alone beneath the Wrangell volcanoes, because the overlying Yakutat terrane has been left behind as underplated rocks beneath the rising St. Elias Range, in the coastal region. West of the tear, the Yakutat terrane and Pacific oceanic lithosphere subduct together at a gentle angle, and this thickened package inhibits volcanism. ?? 2008 The Geological Society of America.

  10. Crustal structure of the western transect of the Rif Cordillera from broadband magnetotelluric data

    International Nuclear Information System (INIS)

    Complete text of publication follows. Within the frame of the Topo-Iberia project, the magnetotelluric method has been applied for first time in the western transect of the Rif Cordillera to delineate the electrical crustal structure. Data acquisition has been done in 18 MT measurement sites, with ADU-07 Metronix equipment, including vertical magnetic records. Frequencies range from 4Hz to 32 kHz and acquisition time on each site was from two to three days. The profile extends approximately 110 km in NE-SW trend orthogonal to the main regional geological structures, crossing from the Alboran Sea coast through the major alpine units of the western Rif Cordillera up to the foreland Gharb basin. We have performed a dimensionality analysis using the Wal's and the Bahr's methods, both based on the rotational invariants. The data obtained show variable dimensionalities ranging from 1D to 3D. Predominant strikes suggest NW-SE orientations. Therefore several 2D models have processed using Winglink software with different rotations. The model with N145 deg E rotation shows a good correlation between the resistive structures and the surface geology, as well as their continuity in depth. The Internal Zones corresponds generally to resistive rocks (metamorphic rocks) including a significant conductive body that probably corresponds to the nearby outcropping Beni Boussera peridotites. The External Zones and the foreland basin are characterized by shallow heterogeneous conductive structures. In depth, the most relevant feature corresponds to a large resistive body located below the frontal part of the Rif. The presence of exotic gneiss blocks in the front of the External Zones suggests that this large deep body may correspond to a gneissic or granitic basement surrounded by metapelitic rocks. The deep structure of this transect contributes to highlight the recent evolution and development of the southern part of the Gibraltar Arch in the frame of the Eurasian-African convergent

  11. Functional recovery after transection of the sciatic nerve at an early age : a pilot study in rats

    NARCIS (Netherlands)

    Meek, Marcel F.; IJkema-Paassen, Jos; Gramsbergen, Albert

    2007-01-01

    It is often clinically noted that peripheral nerve transections in infants and very young children seem to show better functional recovery after nerve repair compared with adult patients. There can be several reasons for this (e.g. outgrowing axons might have maintained their potential to locate the

  12. Topographic, bioclimatic, and vegetation characteristics of three ecoregion classification systems in North America: Comparisons along continent-wide transects

    Science.gov (United States)

    Thompson, R.S.; Shafer, S.L.; Anderson, K.H.; Strickland, L.E.; Pelltier, R.T.; Bartlein, P.J.; Kerwin, M.W.

    2005-01-01

    Ecoregion classification systems are increasingly used for policy and management decisions, particularly among conservation and natural resource managers. A number of ecoregion classification systems are currently available, with each system defining ecoregions using different classification methods and different types of data. As a result, each classification system describes a unique set of ecoregions. To help potential users choose the most appropriate ecoregion system for their particular application, we used three latitudinal transects across North America to compare the boundaries and environmental characteristics of three ecoregion classification systems [Ku??chler, World Wildlife Fund (WWF), and Bailey]. A variety of variables were used to evaluate the three systems, including woody plant species richness, normalized difference in vegetation index (NDVI), and bioclimatic variables (e.g., mean temperature of the coldest month) along each transect. Our results are dominated by geographic patterns in temperature, which are generally aligned north-south, and in moisture, which are generally aligned east-west. In the west, the dramatic changes in physiography, climate, and vegetation impose stronger controls on ecoregion boundaries than in the east. The Ku??chler system has the greatest number of ecoregions on all three transects, but does not necessarily have the highest degree of internal consistency within its ecoregions with regard to the bioclimatic and species richness data. In general, the WWF system appears to track climatic and floristic variables the best of the three systems, but not in all regions on all transects. ?? 2005 Springer Science+Business Media, Inc.

  13. The Point Count Transect Method for Estimates of Biodiversity on Coral Reefs: Improving the Sampling of Rare Species.

    Science.gov (United States)

    Roberts, T Edward; Bridge, Thomas C; Caley, M Julian; Baird, Andrew H

    2016-01-01

    Understanding patterns in species richness and diversity over environmental gradients (such as altitude and depth) is an enduring component of ecology. As most biological communities feature few common and many rare species, quantifying the presence and abundance of rare species is a crucial requirement for analysis of these patterns. Coral reefs present specific challenges for data collection, with limitations on time and site accessibility making efficiency crucial. Many commonly used methods, such as line intercept transects (LIT), are poorly suited to questions requiring the detection of rare events or species. Here, an alternative method for surveying reef-building corals is presented; the point count transect (PCT). The PCT consists of a count of coral colonies at a series of sample stations, located at regular intervals along a transect. In contrast the LIT records the proportion of each species occurring under a transect tape of a given length. The same site was surveyed using PCT and LIT to compare species richness estimates between the methods. The total number of species increased faster per individual sampled and unit of time invested using PCT. Furthermore, 41 of the 44 additional species recorded by the PCT occurred ≤ 3 times, demonstrating the increased capacity of PCT to detect rare species. PCT provides a more accurate estimate of local-scale species richness than the LIT, and is an efficient alternative method for surveying reef corals to address questions associated with alpha-diversity, and rare or incidental events.

  14. The Point Count Transect Method for Estimates of Biodiversity on Coral Reefs: Improving the Sampling of Rare Species.

    Science.gov (United States)

    Roberts, T Edward; Bridge, Thomas C; Caley, M Julian; Baird, Andrew H

    2016-01-01

    Understanding patterns in species richness and diversity over environmental gradients (such as altitude and depth) is an enduring component of ecology. As most biological communities feature few common and many rare species, quantifying the presence and abundance of rare species is a crucial requirement for analysis of these patterns. Coral reefs present specific challenges for data collection, with limitations on time and site accessibility making efficiency crucial. Many commonly used methods, such as line intercept transects (LIT), are poorly suited to questions requiring the detection of rare events or species. Here, an alternative method for surveying reef-building corals is presented; the point count transect (PCT). The PCT consists of a count of coral colonies at a series of sample stations, located at regular intervals along a transect. In contrast the LIT records the proportion of each species occurring under a transect tape of a given length. The same site was surveyed using PCT and LIT to compare species richness estimates between the methods. The total number of species increased faster per individual sampled and unit of time invested using PCT. Furthermore, 41 of the 44 additional species recorded by the PCT occurred ≤ 3 times, demonstrating the increased capacity of PCT to detect rare species. PCT provides a more accurate estimate of local-scale species richness than the LIT, and is an efficient alternative method for surveying reef corals to address questions associated with alpha-diversity, and rare or incidental events. PMID:27011368

  15. A review of the transect method by comparing it with three other counting methods to estimate rabbit (Oryctolagus cuniculus) density

    NARCIS (Netherlands)

    Bankert, D.; Groen, in 't K.C.G.; Wieren, van S.E.

    2003-01-01

    In dune areas in the Netherlands, the standard method for estimating rabbit (Oryctolagus cuniculus) populations is by counting the number of rabbits that can be seen in the headlights of a car along standard transects. This paper evaluates whether estimated rabbit densities derived from this method

  16. FBSAB PREDATOR Reef Fish Belt Transect Surveys at Hawaii Island (Big Island), Main Hawaiian Islands, 2009 (NODC Accession 0073870)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Shore-based belt transects were conducted at 1 to ~5 m depths at a total two (2) sites: at 2 longshore sites on the leeward coast (South Kohala district) of the Big...

  17. Continental-scale patterns in soil geochemistry and mineralogy: results from two transects across the United States and Canada

    Science.gov (United States)

    Woodruff, L.G.; Cannon, W.F.; Eberl, D.D.; Smith, D.B.; Kilburn, J.E.; Horton, J.D.; Garrett, R.G.; Klassen, R.A.

    2009-01-01

    In 2004, the US Geological Survey (USGS) and the Geological Survey of Canada (GSC) initiated a pilot study that involved collection of more than 1500 soil samples from 221 sites along two continental transects across Canada and the United States. The pilot study was designed to test and refine protocols for a soil geochemical survey of North America. The two transects crossed a wide array of soil parent materials, soil ages, climatic conditions, landforms, land covers and land uses. Sample sites were selected randomly at approximately 40-km intervals from a population defined as all soils of the continent. At each site, soils representing 0 to 5 cm depth, and the O, A, and C horizons, if present, were collected and analyzed for their near-total content of over 40 major and trace elements. Soils from 0–5 cm depth were also collected for analysis of organic compounds. Results from the transects confirm that soil samples collected at a 40-km spacing reveal coherent, continental- to subcontinental-scale geochemical and mineralogical patterns that can be correlated to aspects of underlying soil parent material, soil age and climate influence. The geochemical data also demonstrate that at the continental-scale the dominance of any of these major factors that control soil geochemistry can change across the landscape. Along both transects, soil mineralogy and geochemistry change abruptly with changes in soil parent materials. However, the chemical influence of a soil’s parent material can be obscured by changing climatic conditions. For the transects, increasing precipitation from west to east and increasing temperature from north to south affect both soil mineralogy and geochemistry because of climate effects on soil weathering and leaching, and plant productivity. Regional anomalous metal concentrations can be linked to natural variations in soil parent materials, such as high Ni and Cr in soils developed on ultramafic rocks in California or high P in soils formed on

  18. Synchrotron X ray induced axonal transections in the brain of rats assessed by high-field diffusion tensor imaging tractography.

    Science.gov (United States)

    Serduc, Raphaël; Bouchet, Audrey; Pouyatos, Benoît; Renaud, Luc; Bräuer-Krisch, Elke; Le Duc, Géraldine; Laissue, Jean A; Bartzsch, Stefan; Coquery, Nicolas; van de Looij, Yohan

    2014-01-01

    Since approximately two thirds of epileptic patients are non-eligible for surgery, local axonal fiber transections might be of particular interest for them. Micrometer to millimeter wide synchrotron-generated X-ray beamlets produced by spatial fractionation of the main beam could generate such fiber disruptions non-invasively. The aim of this work was to optimize irradiation parameters for the induction of fiber transections in the rat brain white matter by exposure to such beamlets. For this purpose, we irradiated cortex and external capsule of normal rats in the antero-posterior direction with a 4 mm×4 mm array of 25 to 1000 µm wide beamlets and entrance doses of 150 Gy to 500 Gy. Axonal fiber responses were assessed with diffusion tensor imaging and fiber tractography; myelin fibers were examined histopathologically. Our study suggests that high radiation doses (500 Gy) are required to interrupt axons and myelin sheaths. However, a radiation dose of 500 Gy delivered by wide minibeams (1000 µm) induced macroscopic brain damage, depicted by a massive loss of matter in fiber tractography maps. With the same radiation dose, the damage induced by thinner microbeams (50 to 100 µm) was limited to their paths. No macroscopic necrosis was observed in the irradiated target while overt transections of myelin were detected histopathologically. Diffusivity values were found to be significantly reduced. A radiation dose ≤ 500 Gy associated with a beamlet size of < 50 µm did not cause visible transections, neither on diffusion maps nor on sections stained for myelin. We conclude that a peak dose of 500 Gy combined with a microbeam width of 100 µm optimally induced axonal transections in the white matter of the brain.

  19. Synchrotron X ray induced axonal transections in the brain of rats assessed by high-field diffusion tensor imaging tractography.

    Directory of Open Access Journals (Sweden)

    Raphaël Serduc

    Full Text Available Since approximately two thirds of epileptic patients are non-eligible for surgery, local axonal fiber transections might be of particular interest for them. Micrometer to millimeter wide synchrotron-generated X-ray beamlets produced by spatial fractionation of the main beam could generate such fiber disruptions non-invasively. The aim of this work was to optimize irradiation parameters for the induction of fiber transections in the rat brain white matter by exposure to such beamlets. For this purpose, we irradiated cortex and external capsule of normal rats in the antero-posterior direction with a 4 mm×4 mm array of 25 to 1000 µm wide beamlets and entrance doses of 150 Gy to 500 Gy. Axonal fiber responses were assessed with diffusion tensor imaging and fiber tractography; myelin fibers were examined histopathologically. Our study suggests that high radiation doses (500 Gy are required to interrupt axons and myelin sheaths. However, a radiation dose of 500 Gy delivered by wide minibeams (1000 µm induced macroscopic brain damage, depicted by a massive loss of matter in fiber tractography maps. With the same radiation dose, the damage induced by thinner microbeams (50 to 100 µm was limited to their paths. No macroscopic necrosis was observed in the irradiated target while overt transections of myelin were detected histopathologically. Diffusivity values were found to be significantly reduced. A radiation dose ≤ 500 Gy associated with a beamlet size of < 50 µm did not cause visible transections, neither on diffusion maps nor on sections stained for myelin. We conclude that a peak dose of 500 Gy combined with a microbeam width of 100 µm optimally induced axonal transections in the white matter of the brain.

  20. The Crustal Structure of the Central Iberian Zone form the ALCUDIA Deep Seismic Reflection transect.

    Science.gov (United States)

    Martí, D.

    2009-04-01

    The ALCUDIA transect is a 250 km long, vertical incidence Vibroseis seismic reflection profile acquired in 2007. It extends IBERSEIS transect to the N and NE imaging from within the Ossa Morena Zone (OMZ) to the Central Iberian Zone (CIZ) from 20 km south of Fuenteovejuna in the S to Toledo in the N. The southern part of the transect samples the suture zone between the OMZ and the CIZ. It continues in a N-NE direction crossing the Pedroches batholith and a series of relatively long wavelength synclinal structures limited by sub-vertical and relatively narrow folds (e.g. the Almaden syncline, the Alcudia anticline). Lower Paleozoic quartzites and slates cover most of these synclynal structures. Farther to the N, the profile crosses several major faults system (e.g Santa Elena and Toledo) . The acquisition parameters, 35 m station spacing, 70 m VP spacing resulted in a 60-90 fold high resolution seismic reflection image. A 20 s long Vibroseis sweep with frequencies between 8-120 Hz was recorded by a 400 station recording cable a long a 14 km long split spread configuration. The new processing sequence significantly increased the signal-to-noise ratio. It includes: crooked line geometry, geometrical divergence corrections, elevation statics, surface-wave attenuation, surface consistent zero-phase spiking deconvolution, time-variant band pass filtering., refraction and residual static corrections velocity analysis, NMO, surface consistent amplitude balancing, CMP stacking F-X deconvolution. The seismic image reveals the geometry of the suture between the OMZ and the CIZ. This is a reworked transpression suture (the Badajoz-Cordoba Sherar zone) includes the Central Unit (CU) as a north dipping wedge structure limited by two bands of reflectors that reach the middle crust (5 s twtt). This CU includes amphibolites with some oceanic affinity, orthogneisses, paragneisses, schists and minor amounts of peridotites. To the north the upper crust shows a moderate reflectivity

  1. Potential of Gdgts as Temperature Proxies Along Altitudinal Transects in East Africa

    Science.gov (United States)

    Coffinet, Sarah; Huguet, Arnaud; Omuombo, Christine; Williamson, David; Fosse, Céline; Anquetil, Christine; Derenne, Sylvie

    2014-05-01

    Glycerol dialkyl glycerol tetraethers (GDGTs) are lipids of high molecular weight and include the isoprenoid GDGTs (iGDGTs) produced by Archaea and the branched GDGTs (brGDGTs) produced by unknown bacteria. Several indices were developed to describe the relationship between GDGT distribution and environmental parameters: the TEX86 (tetraether index of tetraethers consisting of 86 carbons), based on the relative abundances of iGDGTs in sediments, and the MBT (methylation index of branched tetraethers) and CBT (cyclisation ratio of branched tetraethers), based on the relative abundance of brGDGTs in soils. The TEX86 was shown to correlate well with water surface temperature, and the MBT and CBT with mean annual air temperature (MAAT) and soil pH. The GDGTs are increasingly used as temperature proxies. In this study, 41 surface soils were sampled along two altitudinal transects, from 500 to 2800 meters in Mount Rungwe (South western, Tanzania) and from 1897 to 3268 meters in Mount Kenya (Central Kenya). MAAT was reconstructed along the two transects using the MBT/CBT proxies. A linear correlation between the MBT/CBT-derived temperatures and the altitude (R2=0.83) was obtained by combining results of the two transects. The reconstructed temperature lapse rate (0.5 ° C/100 m) was consistent with the one determined from temperature measurements at six altitudes. These results show that the MBT/CBT is a suitable and robust temperature proxy in East Africa. In Mt. Rungwe soil samples, the TEX86 index, which was mainly used to reconstruct water surface temperatures until now, was found to vary linearly with altitude (R2=0.50). Such a relationship between TEX86 and altitude in organic soils has also been recently noticed in Mt. Xiangpi, China (Liu et al., 2013; R2=0.68). The adiabatic cooling of air with altitude could explain the TEX86 variation with altitude. If such a relationship is confirmed, the use of the TEX86 as a temperature proxy could be extended to soil

  2. Spinal cord transection-induced allodynia in rats--behavioral, physiopathological and pharmacological characterization.

    Directory of Open Access Journals (Sweden)

    Saïd M'Dahoma

    Full Text Available In humans, spinal cord lesions induce not only major motor and neurovegetative deficits but also severe neuropathic pain which is mostly resistant to classical analgesics. Better treatments can be expected from precise characterization of underlying physiopathological mechanisms. This led us to thoroughly investigate (i mechanical and thermal sensory alterations, (ii responses to acute treatments with drugs having patent or potential anti-allodynic properties and (iii the spinal/ganglion expression of transcripts encoding markers of neuronal injury, microglia and astrocyte activation in rats that underwent complete spinal cord transection (SCT. SCT was performed at thoracic T8-T9 level under deep isoflurane anaesthesia, and SCT rats were examined for up to two months post surgery. SCT induced a marked hyper-reflexia at hindpaws and strong mechanical and cold allodynia in a limited (6 cm2 cutaneous territory just rostral to the lesion site. At this level, pressure threshold value to trigger nocifensive reactions to locally applied von Frey filaments was 100-fold lower in SCT- versus sham-operated rats. A marked up-regulation of mRNAs encoding ATF3 (neuronal injury and glial activation markers (OX-42, GFAP, P2×4, P2×7, TLR4 was observed in spinal cord and/or dorsal root ganglia at T6-T11 levels from day 2 up to day 60 post surgery. Transcripts encoding the proinflammatory cytokines IL-1β, IL-6 and TNF-α were also markedly but differentially up-regulated at T6-T11 levels in SCT rats. Acute treatment with ketamine (50 mg/kg i.p., morphine (3-10 mg/kg s.c. and tapentadol (10-20 mg/kg i.p. significantly increased pressure threshold to trigger nocifensive reaction in the von Frey filaments test, whereas amitriptyline, pregabalin, gabapentin and clonazepam were ineffective. Because all SCT rats developed long lasting, reproducible and stable allodynia, which could be alleviated by drugs effective in humans, thoracic cord transection might be a

  3. Post-LIA glacier changes along a latitudinal transect in the Central Italian Alps

    Science.gov (United States)

    Scotti, R.; Brardinoni, F.; Crosta, G. B.

    2014-12-01

    The variability of glacier response to atmospheric temperature rise in different topo-climatic settings is still a matter of debate. To address this question in the Central Italian Alps, we compile a post-LIA (Little Ice Age) multitemporal glacier inventory (1860-1954-1990-2003-2007) along a latitudinal transect that originates north of the continental divide in the Livigno Mountains and extends south through the Disgrazia and Orobie ranges, encompassing continental-to-maritime climatic settings. In these sub-regions, we examine the area change of 111 glaciers. Overall, the total glacierized area has declined from 34.1 to 10.1 km2, with a substantial increase in the number of small glaciers due to fragmentation. The average annual decrease (AAD) in glacier area has risen by about 1 order of magnitude from 1860-1990 (Livigno: 0.45; Orobie: 0.42; and Disgrazia: 0.39 % a-1) to 1990-2007 (Livigno: 3.08; Orobie: 2.44; and Disgrazia: 2.27 % a-1). This ranking changes when considering glaciers smaller than 0.5 km2 only (i.e., we remove the confounding caused by large glaciers in Disgrazia), so that post-1990 AAD follows the latitudinal gradient and Orobie glaciers stand out (Livigno: 4.07; Disgrazia: 3.57; and Orobie: 2.47 % a-1). More recent (2007-2013) field-based mass balances in three selected small glaciers confirm post-1990 trends showing the consistently highest retreat in continental Livigno and minimal area loss in maritime Orobie, with Disgrazia displaying transitional behavior. We argue that the recent resilience of glaciers in Orobie is a consequence of their decoupling from synoptic atmospheric temperature trends, a decoupling that arises from the combination of local topographic configuration (i.e., deep, north-facing cirques) and high winter precipitation, which ensures high snow-avalanche supply, as well as high summer shading and sheltering. Our hypothesis is further supported by the lack of correlations between glacier change and glacier attributes in

  4. Spatial variability of detrended soil plow layer penetrometer resistance transect in a sugarcane field

    Science.gov (United States)

    Pérez, Luis D.; Cumbrera, Ramiro; Mato, Juan; Millán, Humberto; Tarquis, Ana M.

    2015-04-01

    Spatial variability of soil properties is relevant for identifying those zones with physical degradation. In this sense, one has to face the problem of identifying the origin and distribution of spatial variability patterns (Brouder et al., 2001; Millán et al., 2012). The objective of the present work was to quantify the spatial structure of soil penetrometer resistance (PR) collected from a transect data consisted of 221 points equidistant. In each sampling, readings were obtained from 0 cm till 70 cm of depth, with an interval of 5 cm (Pérez, 2012). The study was conducted on a Vertisol (Typic Hapludert) dedicated to sugarcane (Saccharum officinarum L.) production during the last sixty years (Pérez et al., 2010). Recently, scaling approach has been applied on the determination of the scaling data properties (Tarquis et al., 2008; Millán et al., 2012; Pérez, 2012). We focus in the Hurst analysis to characterize the data variability for each depth. Previously a detrended analysis was conducted in order to better study de intrinsic variability of the series. The Hurst exponent (H) for each depth was estimated showing a characteristic pattern and differentiating PR evolution in depth. References Brouder, S., Hofmann, B., Reetz, H.F., 2001. Evaluating spatial variability of soil parameters for input management. Better Crops 85, 8-11. Millán, H; AM Tarquís, Luís D. Pérez, Juan Mato, Mario González-Posada, 2012. Spatial variability patterns of some Vertisol properties at a field scale using standardized data. Soil and Tillage Research, 120, 76-84. Pérez, Luís D. 2012. Influencia de la maquinaria agrícola sobre la variabilidad espacial de la compactación del suelo. Aplicación de la metodología geoestadística-fractal. PhD thesis, UPM (In Spanish). Pérez, Luís D., Humberto Millán, Mario González-Posada 2010. Spatial complexity of soil plow layer penetrometer resistance as influenced by sugarcane harvesting: A prefractal approach. Soil and Tillage

  5. Post-LIA glacier changes along a latitudinal transect in the Central Italian Alps

    Directory of Open Access Journals (Sweden)

    R. Scotti

    2014-07-01

    Full Text Available The variability of glacier response to atmospheric temperature rise in different topo-climatic settings is still matter of debate. To address this question in the Central Italian Alps we compile a post-LIA (Little Ice Age multitemporal glacier inventory (1860-1954-1990-2003-2007 along a latitudinal transect that originates north of the continental divide in the Livigno mountains, and extends south through the Disgrazia and Orobie ranges, encompassing continental-to-maritime climatic settings. In these sub-regions we examine area change of 111 glaciers. Overall, total glacierized area has declined from 34.1 to 10.1 km2, with a substantial increase in the number of small glaciers due to fragmentation. Average annual decrease (AAD in glacier area has risen of about an order of magnitude from 1860–1990 (Livigno: 0.45; Orobie: 0.42; and Disgrazia: 0.39 % a−1 to 1990–2007 (Livigno: 3.08; Orobie: 2.44; and Disgrazia: 2.27 % a−1. This ranking changes when considering glaciers 2 only (i.e., we remove the confounding caused by large glaciers in Disgrazia, so that post-1990 AAD follows the latitudinal gradient and Orobie glaciers stand out (Livigno: 4.07; Disgrazia: 3.57; and Orobie: 2.47 % a−1. More recent (2007–2013 field-based mass balances in three selected small glaciers confirm post-1990 trends showing consistent highest retreat in continental Livigno and minimal area loss in maritime Orobie, with Disgrazia displaying a transitional behaviour. We argue that the recent resilience of glaciers in Orobie is a consequence of their decoupling from synoptic atmospheric temperature trends. A decoupling that arises from the combination of local topographic configuration (i.e., deep, north-facing cirques and high winter precipitation, which ensures high snow-avalanche supply, as well as high summer shading and sheltering. Our hypothesis is further supported by the lack of correlations between glacier change and glacier attributes in Orobie, as well

  6. Phosphorus speciation and transformation along transects in the Benguela upwelling region

    Science.gov (United States)

    Nausch, Monika; Nausch, Günther

    2014-11-01

    Transformation of phosphate (PO4) introduced by upwelling into the 20 m surface layer was studied four times in turn along a transect perpendicular to the coast of Namibia from August 27th to September 15th 2011. [33P]PO4 uptake rates as well as the concentrations of inorganic nutrients (PO4, NO2/3), dissolved and particulate organic phosphorus, and particulate organic carbon and nitrogen were measured and the respective stoichiometry was determined. The fate and interactions of these components are described in relation to both the distance from the coast and a calculated "pseudoage" of the water masses to get knowledge about the phosphorus dynamics during surface transport and aging of upwelled water. PO4 decreased from 1.6 μM in the upwelling cell near the coast to 0.4 μM at remote stations. The decrease in PO4 was lower than that of nitrate, resulting in a lower N:P ratio seawards (from 12-16 to 5-9). PO4 depletion was reflected only partially in increasing DOP, but not in POP concentrations. Concentrations of POP, POC and PON decreased with the distance from the coast and with "pseudoage", indicating that produced particulate matter is removed from the upper 20 m layer. A mean PO4 turnover time of 57 days, based on [33P]PO4 uptake measurements, suggested complete PO4 consumption within the transects and thus the need for an additional PO4 supply to sustain the gradient. Fast mineralisation of DOP could be one mechanism. Deduced from our bioassays, PO4 seemed to be transformed into DOP by heterotrophic bacteria which was mineralized back to PO4 within few days. Hence, our results support the hypothesis that the observed PO4 gradient in the 20 m surface layer is not only due to input in the upwelling center and locomotion to the open ocean by Ekman transport combined with utilization by phyto- and bacterioplankton. PO4 has to be provided by remineralisation and input from deeper layers probably by wind curl driven mixing processes. However, most of the

  7. Ex vivo and in vivo evaluation of an ultrasonic device for precise dissection, coagulation, and transection

    Directory of Open Access Journals (Sweden)

    Bertke BD

    2014-12-01

    Full Text Available Brian D Bertke,1 Patrick J Scoggins,1 Alissa L Welling,1 Tamara V Widenhouse,1 Chaoyang Chen,2 Srinivasu Kallakuri,2 John M Cavanaugh,2 Jeffrey W Clymer,1 Joseph F Amaral1 1Ethicon, Inc., Cincinnati, OH, USA; 2Spine Research Laboratory, Bioengineering Center, Wayne State University, Detroit, MI, USA Background: A new ultrasonic device, Harmonic Focus®+, has been developed that is smaller and delivers energy more efficiently than its predecessor via the inclusion of Adaptive Tissue Technology. This study was undertaken to compare its dissection capabilities to an advanced bipolar electrosurgery device in benchtop and preclinical evaluations. Methods: In ex vivo testing, Focus+ and LigaSure™ Small Jaw were evaluated for physical dimensions, device and tissue temperature after repeated applications to porcine jejunum, and burst pressure of vessel seals, transection time, and tissue sticking in 3–5 mm porcine carotid arteries. In in vivo testing, the devices were tested on intact porcine carotid arteries for thermal damage via collagen denaturation and in muscle incisions near rat sciatic nerve for acute inflammation via hematoxylin and eosin and for impaired axonal transport via β-APP. Results: Focus+ was smaller than the Small Jaw in width and height, yet it had a longer active blade and larger jaw aperture. Device temperatures were not different after application, but thermal spread (tissue temperature above 50°C was 78% greater for Small Jaw (9.6 mm than for Focus+ (5.4 mm. Burst pressures of sealed vessels were not significantly different between the devices: 900 (±466 mmHg for Focus+ versus 974 (±500 mmHg for Small Jaw. Small Jaw had a shorter individual transection time (5.0 seconds compared to 6.3 seconds for Focus+, whereas Focus+ had 70% less tissue sticking. Thermal damage, neural inflammation, and impaired axonal transport were all significantly lower for Focus+ compared to Small Jaw, by 19%, 57%, and 50%, respectively

  8. The Crust and Mantle Relationships Beneath Central and Southern Iberian Peninsula constrained by a 550 km long multiseismic transect

    Science.gov (United States)

    Ehsan, Siddique Akhtar; Carbonell, Ramon; Simancas, Jose Fernando; Martinez Poyatos, David; Azor, Antonio; Ayarza, Puy; Storti, Fabrizio

    2013-04-01

    A composite lithospheric cross section which is composed by data from controlled source multiseismic experiments strongly constrains the lithospheric structure of southwestern Iberia. The data includes coincident normal incidence and wide-angle profiles along an, approximately, 550 km long transect. This transect goes across, from North-to-South, the major tectonic zones that build up Southwestern part of the Iberian Peninsula (the Central Iberian Zone -CIZ-, the Ossa-Morena Zone -OMZ- and the South Portuguese Zone -SPZ-). The knowledge provided by these datasets constitutes the base to develop multidisciplinary models of the lithosphere. The multichannel deep seismic high resolution (60-90 fold) profiles, IBERSEIS & ALCUDIA were acquired in summer 2001 and 2007 are about 300 and 250 km long respectively. The transects image 20 s (TWTT), about 70 km depth. To address the crust and upper mantle structural relationships a reassessment of the normal incidence seismic reflection transect ALCUDIA has been carried out. We revised the key processing steps and applied advance analysis on the ALCUDIA transect with the aim to improve the signal to noise ratio especially in the deep parts and to produce a depth migrated image. The velocity model generated through wide-angle seismic survey (2003) was used to convert IBERSEIS time migrated stack image into depth. The new data processing flow provide better structural constraints on the shallow and deep structures as the current images reveal indentation features which strongly suggest horizontal tectonics. The ALCUDIA transect shows slightly less reflective upper crust about 13 km thick decoupled from the comparatively reflective lower crust. The reflectivity of the lower crust is continuous, high amplitude, horizontal and parallel though evidences of deformation are present as flat-ramp-flat geometry on the northeastern portion and a "Crocodile structure" wedging into the upper mantle on the southwestern portion of the ALCUDIA

  9. Linking primary production, climate and land use along an urban-wildland transect: a satellite view

    International Nuclear Information System (INIS)

    Variation of green vegetation cover influences local climate dynamics, exchange of water-heat between land and atmosphere, and hydrological processes. However, the mechanism of interaction between vegetation and local climate change in subtropical areas under climate warming and anthropogenic disturbances is poorly understood. We analyzed spatial-temporal trends of vegetation with moderate-resolution imaging spectroradiometer (MODIS) vegetation index datasets over three sections, namely urban, urban-rural fringe and wildland along an urban-wildland transect in a southern mega-city area in China from 2000-2008. The results show increased photosynthetic activity occurred in the wildland and the stable urban landscape in correspondence to the rising temperature, and a considerable decrease of vegetation activity in the urban-rural fringe area, apparently due to urban expansion. On analyzing the controlling factors of climate change and human drivers of vegetation cover change, we found that temperature contributed to vegetation growth more than precipitation and that rising temperature accelerated plant physiological activity. Meanwhile, human-induced dramatic modification of land cover, e.g. conversion of natural forest and cropland to built-up areas in the urban-rural fringe, has caused significant changes of green vegetation fraction and overall primary production, which may further influence local climate.

  10. Effects of Sevoflurane on the discharges of wide dynamic range neurons in spinally transected rats

    Institute of Scientific and Technical Information of China (English)

    WANG Ying-wei; XIONG Yuan-chang; DENG Xiao-ming; ZHAO Zhi-qi

    2004-01-01

    Objective: To study the effects of clinical concentration of sevoflurane on activity of wide dynamic range neurons. Methods: Eight Spraque-Dawley rats(male) were selected. Their spinal cords were exposed and transected at T9- 10 level. The rate of firings of single neurons in the dorsal horn in response to electrical stimulation of skin was recorded with microelectrodes. The early and late discharges were observed when rats inhaled 0.5%, 1.0%, 1.5%, and 2.0%sevoflurane. Results: Sevoflurane suppressed the early and late discharges at the concentration of 0.5%, 1.0%, 1.5%,and 2.0%. Compared with early discharges, the extent of inhibition of late discharges was wider at the concentration of1%, 1.5 %, and 2.0% of sevoflurane. Conclusion: It is indicated that sevoflurane could suppress the transmission of nociceptive and non-nociceptive stimulation at dorsal horn. The suppression on nociceptive imput is stronger than that on nonnociceptive imput when the concentration of sevoflurane is more than 1%.

  11. Linking primary production, climate and land use along an urban-wildland transect: a satellite view

    Science.gov (United States)

    Hu, Yonghong; Jia, Gensuo; Guo, Huadong

    2009-10-01

    Variation of green vegetation cover influences local climate dynamics, exchange of water-heat between land and atmosphere, and hydrological processes. However, the mechanism of interaction between vegetation and local climate change in subtropical areas under climate warming and anthropogenic disturbances is poorly understood. We analyzed spatial-temporal trends of vegetation with moderate-resolution imaging spectroradiometer (MODIS) vegetation index datasets over three sections, namely urban, urban-rural fringe and wildland along an urban-wildland transect in a southern mega-city area in China from 2000-2008. The results show increased photosynthetic activity occurred in the wildland and the stable urban landscape in correspondence to the rising temperature, and a considerable decrease of vegetation activity in the urban-rural fringe area, apparently due to urban expansion. On analyzing the controlling factors of climate change and human drivers of vegetation cover change, we found that temperature contributed to vegetation growth more than precipitation and that rising temperature accelerated plant physiological activity. Meanwhile, human-induced dramatic modification of land cover, e.g. conversion of natural forest and cropland to built-up areas in the urban-rural fringe, has caused significant changes of green vegetation fraction and overall primary production, which may further influence local climate.

  12. Nitrogen fixation in sediments along a depth transect through the Peruvian oxygen minimum zone

    Science.gov (United States)

    Gier, Jessica; Sommer, Stefan; Löscher, Carolin R.; Dale, Andrew W.; Schmitz, Ruth A.; Treude, Tina

    2016-07-01

    The potential coupling of nitrogen (N2) fixation and sulfate reduction (SR) was explored in sediments of the Peruvian oxygen minimum zone (OMZ). Sediment samples were retrieved by a multiple corer at six stations along a depth transect (70-1025 m water depth) at 12° S, covering anoxic and hypoxic bottom water conditions. Benthic N2 fixation, determined by the acetylene reduction assay, was detected at all sites, with highest rates between 70 and 253 m and lower rates at greater depth. SR rates decreased with increasing water depth. N2 fixation and SR overlapped in sediments, suggesting a potential coupling of both processes. However, a weak positive correlation of their activity distribution was detected by principle component analysis. A potential link between N2 fixation and sulfate-reducing bacteria was indicated by the molecular analysis of nifH genes. Detected nifH sequences clustered with the sulfate-reducing bacteria Desulfonema limicola at the 253 m station. However, nifH sequences of other stations clustered with uncultured organisms, Gammaproteobacteria, and Firmicutes (Clostridia) rather than with known sulfate reducers. The principle component analysis revealed that benthic N2 fixation in the Peruvian OMZ is controlled by organic matter (positive) and free sulfide (negative). No correlation was found between N2 fixation and ammonium concentrations (even at levels > 2022 µM). N2 fixation rates in the Peruvian OMZ sediments were in the same range as those measured in other organic-rich sediments.

  13. A silk sericin/silicone nerve guidance conduit promotes regeneration of a transected sciatic nerve.

    Science.gov (United States)

    Xie, Hongjian; Yang, Wen; Chen, Jianghai; Zhang, Jinxiang; Lu, Xiaochen; Zhao, Xiaobo; Huang, Kun; Li, Huili; Chang, Panpan; Wang, Zheng; Wang, Lin

    2015-10-28

    Peripheral nerve gap defects lead to significant loss of sensory or motor function. Tissue engineering has become an important alternative to nerve repair. Sericin, a major component of silk, is a natural protein whose value in tissue engineering has just begun to be explored. Here, the first time use of sericin in vivo is reported as a long-term implant for peripheral nerve regeneration. A sericin nerve guidance conduit is designed and fabricated. This conduit is highly porous with mechanical strength matching peripheral nerve tissue. It supports Schwann cell proliferation and is capable of up-regulating the transcription of glial cell derived neurotrophic factor and nerve growth factor in Schwann cells. The sericin conduit wrapped with a silicone conduit (sericin/silicone double conduits) is used for bridging repair of a 5 mm gap in a rat sciatic nerve transection model. The sericin/silicone double conduits achieve functional recovery comparable to that of autologous nerve grafting as evidenced by drastically improved nerve function and morphology. Importantly, this improvement is mainly attributed to the sericin conduit as the silicone conduit alone only produces marginal functional recovery. This sericin/silicone-double-conduit strategy offers an efficient and valuable alternative to autologous nerve grafting for repairing damaged peripheral nerve. PMID:26332703

  14. Liquid Metal as Connecting or Functional Recovery Channel for the Transected Sciatic Nerve

    CERN Document Server

    Zhang, Jie; Jin, Chao; Liu, Jing

    2014-01-01

    In this article, the liquid metal GaInSn alloy (67% Ga, 20.5% In, and 12.5% Sn by volume) is proposed for the first time to repair the peripheral neurotmesis as connecting or functional recovery channel. Such material owns a group of unique merits in many aspects, such as favorable fluidity, super compliance, high electrical conductivity, which are rather beneficial for conducting the excited signal of nerve during the regeneration process in vivo. It was found that the measured electroneurographic signal from the transected bullfrog sciatic nerve reconnected by the liquid metal after the electrical stimulation was close to that from the intact sciatic nerve. The control experiments through replacement of GaInSn with the conventionally used Riger Solution revealed that Riger Solution could not be competitive with the liquid metal in the performance as functional recovery channel. In addition, through evaluation of the basic electrical property, the material GaInSn works more suitable for the conduction of the...

  15. The uniformly most powerful invariant test for two models of detection function in point transect sampling

    Directory of Open Access Journals (Sweden)

    Riccardo Borgoni

    2013-05-01

    Full Text Available Estimating population abundance is of primary interest in wildlife population studies. Point transect sampling is a well established methodology for this purpose. The usual approach for estimating the density or the size of the population of interest is to assume a particular model for the detection function (the conditional probability of detecting an animal given that it is at a certain distance from the observer. Two popular models for this function are the half-normal model and the negative exponential model. However, it appears that the estimates are extremely sensitive to the shape of the detection function, particularly to the so-called shoulder condition, which ensures that an animal is nearly certain to be detected if it is at a small distance from the observer. The half-normal model satisfies this condition whereas the negative exponential does not. Testing whether such a hypothesis is consistent with the data at hand should be a primary concern. Given that the problem of testing the shoulder condition of a detection function is invariant under the group of scale transformations, in this paper we propose the uniformly most powerful.

  16. Total gaseous mercury along a transect from coastal to central Antarctic: Spatial and diurnal variations.

    Science.gov (United States)

    Wang, Jiancheng; Zhang, Lulu; Xie, Zhouqing

    2016-11-01

    Total gaseous mercury (TGM) in the atmospheric boundary layer was investigated along a transect from coastal (Zhongshan Station; 69°22'25″S, 76°22'14″E) to central (Kunlun Station; 80°25'2″S, 77°6'47″E) Antarctic from December 16, 2012 to February 6, 2013. TGM varied considerably from 0.32 to 2.34ngm(-3) with a mean value of 0.91ngm(-3). Spatially, relatively high values occurred near the coastal region and on the central plateau with altitude higher than 3000m above sea level. This distribution pattern cannot be accounted for simply by the influence of mercury emission from the ocean. Changes in TGM were also found to be related to the topography. TGM was higher in the inland flat region (290-800km from the coast) than in the inland transition zones with steep slopes (800-1000km from the coast). Temporally, diurnal cycling of TGM was clearly observed at Kunlun Station, with the lowest value occurring typically at midnight, and the peak value at midday. This diurnal pattern was attributed to the reemission of gaseous elemental mercury (GEM) from the snow pack, the oxidization of GEM and convective mixing. PMID:27318733

  17. Total gaseous mercury along a transect from coastal to central Antarctic: Spatial and diurnal variations.

    Science.gov (United States)

    Wang, Jiancheng; Zhang, Lulu; Xie, Zhouqing

    2016-11-01

    Total gaseous mercury (TGM) in the atmospheric boundary layer was investigated along a transect from coastal (Zhongshan Station; 69°22'25″S, 76°22'14″E) to central (Kunlun Station; 80°25'2″S, 77°6'47″E) Antarctic from December 16, 2012 to February 6, 2013. TGM varied considerably from 0.32 to 2.34ngm(-3) with a mean value of 0.91ngm(-3). Spatially, relatively high values occurred near the coastal region and on the central plateau with altitude higher than 3000m above sea level. This distribution pattern cannot be accounted for simply by the influence of mercury emission from the ocean. Changes in TGM were also found to be related to the topography. TGM was higher in the inland flat region (290-800km from the coast) than in the inland transition zones with steep slopes (800-1000km from the coast). Temporally, diurnal cycling of TGM was clearly observed at Kunlun Station, with the lowest value occurring typically at midnight, and the peak value at midday. This diurnal pattern was attributed to the reemission of gaseous elemental mercury (GEM) from the snow pack, the oxidization of GEM and convective mixing.

  18. Spatial distribution patterns in macrobenthos along a latitudinal transect at the deep-sea observatory HAUSGARTEN

    Science.gov (United States)

    Vedenin, A.; Budaeva, N.; Mokievsky, V.; Pantke, C.; Soltwedel, T.; Gebruk, A.

    2016-08-01

    Spatial distribution patterns of benthic organisms are the focus of various faunal marine studies. However, data on horizontal and bathymetric distribution of the deep-sea macrofauna are still scattered and incomplete, and conclusions are usually based on a low number of samples. Spatial distribution of benthic macrofauna was studied based on the material collected during the RV Polarstern expedition ARK-XXVII/2 in July 2012. Eleven stations along the latitudinal transect at the LTER (Long-Term Ecological Research) observatory HAUSGARTEN in the Fram Strait were taken at depths of about 2.3-2.7 km. Macrofauna was obtained using the box corer. A half core (0.125 m2) was taken at each station and four subcores (0.03 m2) were taken from each core and used for the quantitative analysis. The results suggest that a single, highly variable community with the dominance of polychaetes Galathowenia fragilis and Myriochele heeri inhabits the studied area. No latitudinal gradient in the community was revealed. The prevalence of a spatial variability in the community structure at a scale larger than the full sample per station (0.125 m2) was detected. Several abundant taxa (e.g. the polychaetes Prionospio sp. and Galathowenia fragilis) tend to form patches within a full sample.

  19. Moho geometry along a north-south passive seismic transect through Central Australia

    Science.gov (United States)

    Sippl, Christian

    2016-04-01

    Receiver functions from a temporary deployment of 25 broadband stations along a north-south transect through Central Australia are used to retrieve crustal and uppermost mantle structural constraints from a combination of different methods. Using H-K stacking as well as receiver function inversion, overall thick crust with significant thickness variation along the profile (40 to ≥ 55 km) is found. Bulk crustal vp/vs values are largely in the felsic to intermediate range, with the southernmost stations on the Gawler Craton exhibiting higher values in excess of 1.8. A common conversion point (CCP) stacking profile shows three major discontinuities of the crust-mantle boundary: (1) a two-sided Moho downwarp beneath the Musgrave Province, which has previously been associated with the Neoproterozoic to early Cambrian Petermann Orogeny, (2) a Moho offset along the Redbank Shear Zone further north attributed to the Middle to Late Paleozoic Alice Springs Orogeny, and (3) another Moho offset further north, located at the boundary between the Davenport and Warramunga Provinces, which has not been imaged before. In all cases, the difference in crustal thickness between the two sides of the offset is > 8-10 km. Unlike the two southern Moho offsets, the northernmost one does not coincide with a prominent gravity anomaly. Its location and the absence of known reactivation events in the region make it likely that it belongs to a Proterozoic suture zone that marks a previously unknown block boundary within the North Australian Craton.

  20. Downward migration of radiocaesium in organic soils across a transect in Scotland.

    Science.gov (United States)

    Shand, C A; Rosén, K; Thored, K; Wendler, R; Hillier, S

    2013-01-01

    Following the accident at the Chernobyl nuclear power plant in 1986, radioactive materials including (137)Cs were distributed over large parts of the former Soviet Union and Europe. Due to the relatively long physical half-life of (137)Cs (30.1 y) measurable activity concentrations can still be observed in the natural environment. We have determined the depth distribution of (137)Cs from the testing of nuclear weapons in the atmosphere and Chernobyl, in eight highly-organic soils along an approximately east to west transect across the Central Highlands of Scotland. The (137)Cs activity concentrations in the soil profiles, corrected for radioactive decay to May 1986, were between 2.8 and 14.4 kBq m(-2). There were differences in the pattern of distribution of (137)Cs in the soil profiles. The mean migration depth of (137)Cs in the soil profiles was 12.2 cm with a range between 8.2 and 17.4 cm. Quantitative mineralogical analysis of the ash obtained after heating the soil to 400 °C indicated that clay minerals alone did not have a prime role in controlling the migration of (137)Cs down the profile. PMID:22935438

  1. Spatial-temporal Patterns of Land-use Change in Typical Transect Area Along China National Highway 106 During 1996-2008

    Institute of Scientific and Technical Information of China (English)

    LIU Yu; LIU Yansui; GUO Liying; LU Shasha

    2011-01-01

    Based on land-use database updated with the survey of 1996, 2000, 2004 and 2008, this paper analyzed the land-use changes in the typical transect area along National Highway 106 with the aid of GIS technology and quantitative models. The results showed that arable land had been continuously decreased from 1996 to 2008, with a loss of 65.85× 10^3 hm2 and an average decrement of 5.49×10^3 hm^2 per year, and the loss in northern areas was larger; garden land and woodland were gradually centralized to the predominance area with an increment of 25.73×10^3 hm2 and 22.37×10^3 hm2, respectively; residential and industrial land increased year after year, and the transportation land showed the equalized developing spatial pattern; the increment of construction land and encroachment on arable land in the area were deeply influenced by the national macroscopic land-use policies, the rapid advance of urbanization and the development level of the regional economy. Through this case study, it is suggested that differential land- use policies should be adopted to create good environmental conditions to guarantee food security and to promote the economic development.

  2. Morphological and Genetic Variation along a North-to-South Transect in Stipa purpurea, a Dominant Grass on the Qinghai-Tibetan Plateau: Implications for Response to Climate Change.

    Science.gov (United States)

    Liu, Wensheng; Zhao, Yao; You, Jianling; Qi, Danhui; Zhou, Yin; Chen, Jiakuan; Song, Zhiping

    2016-01-01

    Estimating the potential of species to cope with rapid environmental climatic modifications is of vital importance for determining their future viability and conservation. The variation between existing populations along a climatic gradient may predict how a species will respond to future climate change. Stipa purpurea is a dominant grass species in the alpine steppe and meadow of the Qinghai-Tibetan Plateau (QTP). Ecological niche modelling was applied to S. purpurea, and its distribution was found to be most strongly correlated with the annual precipitation and the mean temperature of the warmest quarter. We established a north-to-south transect over 2000 km long on the QTP reflecting the gradients of temperature and precipitation, and then we estimated the morphological by sampling fruited tussocks and genetic divergence by using 11 microsatellite markers between 20 populations along the transect. Reproductive traits (the number of seeds and reproductive shoots), the reproductive-vegetative growth ratio and the length of roots in the S. purpurea populations varied significantly with climate variables. S. purpurea has high genetic diversity (He = 0.585), a large effective population size (Ne >1,000), and a considerable level of gene flow between populations. The S. purpurea populations have a mosaic genetic structure: some distant populations (over 1000 km apart) clustered genetically, whereas closer populations (climate change. PMID:27580056

  3. Modern pollen rain–vegetation relationships along a forest–steppe transect in the Golestan National Park, NE Iran

    OpenAIRE

    Djamali, M.; de Beaulieu, JL; Campagne, P.; H. AKHANI; Andrieu-Ponel, V.; Ponel, P; Leroy, SAG

    2009-01-01

    Pollen rain-vegetation relationships were studied over a forest-steppe transect in Golestan National Park, NE Iran. The surface pollen percentages were compared to the vegetation composition of the respective vegetation types in 18 sampling points using both descriptive and numerical approaches. Hyrcanian lowland forests are characterized by pollen assemblages dominated by Quercus, Carpinus betulus and low frequencies of Zelkova carpinifolia. Both Parrotia persica and Zelkova carpinifolia sho...

  4. A case report of a Wada test after dominant hemisphere multiple hippocampal transections: Pathophysiology of confusion after amobarbital injection

    Directory of Open Access Journals (Sweden)

    Patrick Landazuri

    2014-01-01

    Full Text Available Dialepsis is defined as a predominant alteration of consciousness with preservation of motor tone and the ability to perform movements. While dialepsis is a common feature of both focal and generalized epilepsies, its precise symptomatogenic zone and pathogenesis remain undefined. This case report describes a patient who underwent intracarotid amobarbital procedures before and after dominant hemisphere multiple hippocampal transections. From our observations, we propose a possible pathogenesis for the generation of dialeptic seizures.

  5. Major- and trace-element concentrations in soils from two continental-scale transects of the United States and Canada

    Science.gov (United States)

    Smith, David B.; Cannon, William F.; Woodruff, Laurel G.; Garrett, Robert G.; Klassen, Rodney; Kilburn, James E.; Horton, John D.; King, Harley D.; Goldhaber, Martin B.; Morrison, Jean M.

    2005-01-01

    This report contains major- and trace-element concentration data for soil samples collected from 265 sites along two continental-scale transects in North America. One of the transects extends from northern Manitoba to the United States-Mexico border near El Paso, Tex. and consists of 105 sites. The other transect approximately follows the 38th parallel from the Pacific coast of the United States near San Francisco, Calif., to the Atlantic coast along the Maryland shore and consists of 160 sites. Sampling sites were defined by first dividing each transect into approximately 40-km segments. For each segment, a 1-km-wide latitudinal strip was randomly selected; within each strip, a potential sample site was selected from the most representative landscape within the most common soil type. At one in four sites, duplicate samples were collected 10 meters apart to estimate local spatial variability. At each site, up to four separate soil samples were collected as follows: (1) material from 0-5 cm depth; (2) O horizon, if present; (3) a composite of the A horizon; and (4) C horizon. Each sample collected was analyzed for total major- and trace-element composition by the following methods: (1) inductively coupled plasmamass spectrometry (ICP-MS) and inductively coupled plasma-atomic emission spectrometry (ICPAES) for aluminum, antimony, arsenic, barium, beryllium, bismuth, cadmium, calcium, cerium, cesium, chromium, cobalt, copper, gallium, indium, iron, lanthanum, lead, lithium, magnesium, manganese, molybdenum, nickel, niobium, phosphorus, potassium, rubidium, scandium, silver, sodium, strontium, sulfur, tellurium, thallium, thorium, tin, titanium, tungsten, uranium, vanadium, yttrium, and zinc; (2) cold vapor- atomic absorption spectrometry for mercury; (3) hydride generation-atomic absorption spectrometry for antimony and selenium; (4) coulometric titration for carbonate carbon; and (5) combustion for total carbon and total sulfur.

  6. Thoracic 9 Spinal Transection-Induced Model of Muscle Spasticity in the Rat: A Systematic Electrophysiological and Histopathological Characterization

    OpenAIRE

    Corleto, Jose A.; Mariana Bravo-Hernández; Kota Kamizato; Osamu Kakinohana; Camila Santucci; Navarro, Michael R.; Oleksandr Platoshyn; Dasa Cizkova; Nadezda Lukacova; Julian Taylor; Martin Marsala

    2015-01-01

    The development of spinal hyper-reflexia as part of the spasticity syndrome represents one of the major complications associated with chronic spinal traumatic injury (SCI). The primary mechanism leading to progressive appearance of muscle spasticity is multimodal and may include loss of descending inhibitory tone, alteration of segmental interneuron-mediated inhibition and/or increased reflex activity to sensory input. Here, we characterized a chronic thoracic (Th 9) complete transection mode...

  7. Cruise report for the April 2009 Gulf of Alaska Line-Transect survey (GOALS) in the Navy training exercise area

    OpenAIRE

    Rone, Brenda K.; Douglas, Annie B; Clapham, Phillip; Martinez, Anthony; Morse, Laura J.; Calambokidis, John

    2009-01-01

    The United States Navy, which maintains a maritime training area in the central Gulf of Alaska (GoA), funded a vessel-based line-transect survey during April 2009 to determine marine mammal species distribution and abundance in the training area. The survey cruise employed multiple observation techniques, including visual and passive acoustic observations, as well as photographic identifications. Results of the survey are presented in this document. During the survey cruise, fin whales were...

  8. Crustal Structure Across the Northridge Earthquake Region Using Wide-Angle Seismic Profiling along the LARSE II Transect

    Science.gov (United States)

    Okaya, D.; Fuis, G.; Godfrey, N.; Murphy, J.; Clayton, R.; ten Brink, U.

    2001-12-01

    A crustal transect across the 1994 Northridge earthquake region was completed in 1999 with the acquisition of land reflection/refraction data in LARSE II (Los Angeles Region Seismic Experiment, Phase II). These data are combined with onshore-offshore refraction profiling plus multichannel seismic (MCS) data and ocean bottom seismometer (OBS) data collected in 1994 as part of LARSE I. The total data set forms a complete 280-km onshore-offshore transect from the southern California Borderlands, across the Northridge earthquake region, the Transverse Ranges, the San Andreas fault, the Mojave Desert, and Tehachapi Mts. The onshore-offshore experiment in 1994 used data from 47 land stations (vertical-component Refteks) and eight OBS's in the Borderlands. These instruments collected approx. 150 km of MCS airgun sources produced by the R/V Ewing. The land experiment in 1999 used 93 variable-sized explosions (5-4000 lbs) recorded into an array of portable seismographs 150 km long extending from the Malibu coastline to the Tehachapi Mts. For the southern 90 km, instrument spacing was 100 m including through the urban San Fernando and Santa Clarita Valleys (km 12-47), where LARSE data is augmented by industry reflection and well data. The Mojave Desert segment (km 77-101) also has industry reflection and well data. We present the crustal structure along this transect derived from first arrival traveltime tomography and reflection ray trace modeling. We compare these results to a separate LARSE transect 50-80 km to the east through the Los Angeles Basin and San Gabriel Mts.

  9. Histological modifications of the rat prostate following transection of somatic and autonomic nerves

    Directory of Open Access Journals (Sweden)

    Rosaura Diaz

    2010-06-01

    Full Text Available It is known that hormones influence significantly the prostate tissue. However, we reported that mating induces an increase in androgen receptors, revealing a neural influence on the gland. These data suggested that somatic afferents (scrotal and genitofemoral nerves and autonomic efferents (pelvic and hypogastric nerves could regulate the structure of the prostate. Here we assessed the role of these nerves in maintaining the histology of the gland. Hence, afferent or efferent nerves of male rats were transected. Then, the ventral and dorsolateral regions of the prostate were processed for histology. Results showed that afferent transection affects prostate histology. The alveoli area decreased and increased in the ventral and dorsolateral prostate, respectively. The epithelial cell height increased in both regions. Efferent denervation produced dramatic changes in the prostate gland. The tissue lost its configuration, and the epithelium became scattered and almost vanished. Thus, afferent nerves are responsible for spinal processes pertaining to the trophic control of the prostate, activating its autonomic innervation. Hence, our data imply that innervation seems to be synergic with hormones for the healthy maintenance of the prostate. Thus, it is suggested that some prostate pathologies could be due to the failure of the autonomic neural pathways regulating the gland.Sabe-se que os hormônios influenciam significativamente o tecido prostático. Entretanto, nós demonstramos que o acasalamento induz um aumento nos receptores androgênicos, revelando uma influência neural sobre a glândula. Esses dados sugerem que os aferentes somáticos (nervos escrotal e genito-femural e os eferentes autonômicos (nervos pélvicos e hipo-gástricos poderiam regular a estrutura da próstata. Neste trabalho, avaliou-se a função destes nervos na manutenção da histologia da glândula. Dessa forma, os nervos aferentes e eferentes de ratos machos foram

  10. Evaluation of the combination of multiple subpial transection and other techniques for treatment of intractable epilepsy

    Institute of Scientific and Technical Information of China (English)

    赵全军; 田增民; 刘宗惠; 李士月; 崔月汉; 林鸿

    2003-01-01

    Objective Multiple subpial transection (MST) is one approach to the surgical treatment of intractable epilepsy with epileptogenic lesion located in functional areas. To verify the effect of MST, an experimental study was performed first, followed by clinical application. Methods On the basis of the experimental study, MST was performed in 200 intractable epileptic patients from 1991 to 2000. Of them, 80 cases underwent MST only while 120 others underwent MST combined with other techniques, such as corpus callosotomy, temporal lobectomy and focus resection. A series of modifications of the surgical techniques were made. Results The results of the experimental study indicated that MST could inhibit the formation and spreading of epileptic discharge and limit the damage to neurons in a minimal area on the epileptogenic agent injected cortex. MST does not impair major functions of the cortex. After the clinical application and modifications, 160 patients were followed up for 1 to 8 years. Complete control of seizure was obtained in 100 cases (62.5%), significant reduction (more than 75%) in 32, reduction (more than 50%) in 20 and no change in 8. The total rate of effectiveness was 95.0%, and the significant rate of effectiveness was 82.5%. No functional defects were found in any patients. Conclusions The results indicate that MST is an effective approach to the surgical treatment of intractable epilepsy. MST can be combined with other approaches. The outcome of the subdivision of the MST only group indicates that MST on local epileptogenic lesion without structural changes is as effective as that of the combined operation group. To evade hemispheric disturbance, MST should be done first to avoid severe complications. Hemispherectomy should be performed only on poor effected cases of MST.

  11. Plant species dominance shifts across erosion edge-meadow transects in the Swiss Alps.

    Science.gov (United States)

    Huck, Corinne; Körner, Christian; Hiltbrunner, Erika

    2013-03-01

    While exerting no obvious function under "average" environmental conditions, the presence of certain plant specialists becomes crucial in the event of a complete failure of a community due to severe disturbance such as landslides. Plants capable of growing at erosion edges may act as potential edge-engineers by coping with unstable ground and stabilizing the soil with their roots. We hypothesized that life conditions at erosion edges select for a particular set of specialists or species with specific traits, the identification of which was the aim of the study. Across 17 small-scale transects (0.40 × 1.60 m) from intact meadows to landslide edges (Ursern Valley, Swiss Alps, c. 1,600 m a.s.l.), we quantified plant species abundance by the point intercept method and characterized growth conditions based on Landolt's indicator values, leaf δ(13)C, and volumetric soil moisture in the uppermost soil layers. We observed a clear change of plant species composition and relative abundance from the meadow to the edge, presumably induced by the 25 % lower soil moisture and microclimatic exposure. Species richness at the edge was two-thirds of that in the meadow, but was positively correlated with species richness of the adjacent meadow. Species with "edge-preference" had either (1) rolled or festucoid leaves like Festuca spp., Avenella flexuosa and Nardus stricta, or (2) small, scleromorphic leaves like Vaccinium vitis-idaea, Calluna vulgaris and Thymus ssp. Graminoids with rolled/festucoid leaves were found to be the most dominant edge-specialists. The grass Festuca valesiaca s.l. emerged as the most dominant plant species at the edge, having an 11-times higher cover at the edge than in the meadow. In this montane grassland, a single species contributes to the stabilization of erosion edges and may be regarded as a potential keystone species for slope stability and regeneration after landslides even its role has not so far been established.

  12. Therapy induces widespread reorganization of motor cortex after complete spinal transection that supports motor recovery.

    Science.gov (United States)

    Ganzer, Patrick D; Manohar, Anitha; Shumsky, Jed S; Moxon, Karen A

    2016-05-01

    Reorganization of the somatosensory system and its relationship to functional recovery after spinal cord injury (SCI) has been well studied. However, little is known about the impact of SCI on organization of the motor system. Recent studies suggest that step-training paradigms in combination with spinal stimulation, either electrically or through pharmacology, are more effective than step training alone at inducing recovery and that reorganization of descending corticospinal circuits is necessary. However, simpler, passive exercise combined with pharmacotherapy has also shown functional improvement after SCI and reorganization of, at least, the sensory cortex. In this study we assessed the effect of passive exercise and serotonergic (5-HT) pharmacological therapies on behavioral recovery and organization of the motor cortex. We compared the effects of passive hindlimb bike exercise to bike exercise combined with daily injections of 5-HT agonists in a rat model of complete mid-thoracic transection. 5-HT pharmacotherapy combined with bike exercise allowed the animals to achieve unassisted weight support in the open field. This combination of therapies also produced extensive expansion of the axial trunk motor cortex into the deafferented hindlimb motor cortex and, surprisingly, reorganization within the caudal and even the rostral forelimb motor cortex areas. The extent of the axial trunk expansion was correlated to improvement in behavioral recovery of hindlimbs during open field locomotion, including weight support. From a translational perspective, these data suggest a rationale for developing and optimizing cost-effective, non-invasive, pharmacological and passive exercise regimes to promote plasticity that supports restoration of movement after spinal cord injury. PMID:26826448

  13. Levels of mercury in alligators (Alligator mississippiensis) collected along a transect through the Florida Everglades

    Science.gov (United States)

    Rumbold, D.G.; Fink, L.E.; Laine, K.A.; Niemczyk, S.L.; Chandrasekhar, T.; Wankel, Scott D.; Kendall, C.

    2002-01-01

    As part of a multi-agency study of alligator health, 28 American alligators (Alligator mississippiensis) were captured along a transect through the Florida Everglades in 1999. Liver and tail muscle tissues were sampled and analyzed on a wet weight basis for total mercury (THg) using cold-vapor atomic absorption spectrophotometry. All tissues had detectable concentrations of THg that ranged from 0.6 to 17 mg/kg in liver and from 0.1 to 1.8 mg/kg in tail muscle. THg was more concentrated in liver tissue than tail muscle, but levels were highly correlated between tissues. THg concentrations in tissue differed significantly among locations, with animals from Everglades National Park (ENP) having mean concentrations of THg in liver (10.4 mg/kg) and tail muscle (1.2 mg/kg) that were two-fold higher than basin-wide averages (4.9 and 0.64 mg/kg, respectively). The reasons for higher contamination of ENP alligators were unclear and could not be explained by differences in sex, length, weight or animal age. While ??15N values were positively correlated with THg concentrations in tail muscle, spatial patterns in isotopic composition did not explain the elevated THg levels in ENP alligators. Therefore, it appears that ENP alligators were more highly exposed to mercury in their environment than individuals in other areas. Comparisons to a previous survey by Yanochko et al. [Arch Environ Contam Toxicol 32 (1997) 323] suggest that mercury levels have declined in some Everglades alligators since 1994. ?? 2002 Elsevier Science B.V. All rights reserved.

  14. Data management for support of the Oregon Transect Ecosystem Research (OTTER) project

    Science.gov (United States)

    Skiles, J. W.; Angelici, Gary L.

    1993-01-01

    Management of data collected during projects that involve large numbers of scientists is an often overlooked aspect of the experimental plan. Ecosystem science projects like the Oregon Transect Ecosystem Research (OTTER) Project that involve many investigators from many institutions and that run for multiple years, collect and archive large amounts of data. These data range in size from a few kilobytes of information for such measurements as canopy chemistry and meteorological variables, to hundreds of megabytes of information for such items as views from multi-band spectrometers flown on aircraft and scenes from imaging radiometers aboard satellites. Organizing and storing data from the OTTER Project, certifying those data, correcting errors in data sets, validating the data, and distributing those data to other OTTER investigators is a major undertaking. Using the National Aeronautics and Space Administration's (NASA) Pilot Land Data System (PLDS), a Support mechanism was established for the OTTER Project which accomplished all of the above. At the onset of the interaction between PLDS and OTTER, it was not certain that PLDS could accomplish these tasks in a manner that would aid researchers in the OTTER Project. This paper documents the data types that were collected under the auspices of the OTTER Project and the procedures implemented to store, catalog, validate, and certify those data. The issues of the compliance of investigators with data-management requirements, data use and certification, and the ease of retrieving data are discussed. We advance the hypothesis that formal data management is necessary in ecological investigations involving multiple investigators using many data gathering instruments and experimental procedures. The issues and experience gained in this exercise give an indication of the needs for data management systems that must be addressed in the coming decades when other large data-gathering endeavors are undertaken by the ecological

  15. Prevention of Cartilage Degeneration and Gait Asymmetry by Lubricin Tribosupplementation in the Rat Following ACL Transection

    Science.gov (United States)

    Jay, Gregory D.; Elsaid, Khaled A.; Kelly, Karen A.; Anderson, Scott C.; Zhang, Ling; Teeple, Erin; Waller, Kimberly; Fleming, Braden C.

    2011-01-01

    Objective To investigate whether cartilage degeneration is prevented or minimized in an anterior cruciate ligament (ACL) injury rat model following a single dose-escalated intra-articular injection of lubricin derived from human synoviocytes in culture (HSL). Methods Unilateral ACL transection (ACLT) of the right hindlimb was performed in Lewis rats (N = 56). Control animals underwent a capsulotomy alone leaving the ACL intact (N = 11). Intra-articular injections (50μl/injection) of PBS (N = 14) and HSL (N = 14; 1600μg/ml) were performed on day 7 post-surgery. Animals were euthanized on day 70 post-surgery. Histological specimens were immunoprobed for lubricin, and sulfated glycosaminoglycans. Urinary CTX-II (uCTX-II) levels were measured on day 35 and 70 post-surgery. Hindlimb maximum applied force was determined using a variable resistor walkway to monitor quadruped gait asymmetries. Results Increased immunostaining for lubricin in the superficial zone and on the surface of cartilage was observed in lubricin-treated and control animals but not the PBS-treated nor the untreated ACLT animals. On post-operative day 35 and 70, uCTXII levels of HSL-treated animals were lower than corresponding untreated and PBS-treated (p=0.005; p<0.001 respectively) animals. ACLT animals treated with HSL and control animals distributed their weight equally between hindlimbs compared to PBS treated or untreated animals (p<0.01). Conclusion A single intra-articular injection of concentrated lubricin, following ACLT, reduced collagen type II degradation and improved weight bearing in the affected joint. This study supports the practice of tribosupplementation with lubricin in retarding cartilage degeneration and possibly the development of post-traumatic OA. PMID:22127873

  16. Participation of neuronal nitric oxide synthase in experimental neuropathic pain induced by sciatic nerve transection

    Directory of Open Access Journals (Sweden)

    M. Chacur

    2010-04-01

    Full Text Available Nerve injury leads to a neuropathic pain state that results from central sensitization. This phenomenom is mediated by NMDA receptors and may involve the production of nitric oxide (NO. In this study, we investigated the expression of the neuronal isoform of NO synthase (nNOS in the spinal cord of 3-month-old male, Wistar rats after sciatic nerve transection (SNT. Our attention was focused on the dorsal part of L3-L5 segments receiving sensory inputs from the sciatic nerve. SNT resulted in the development of neuropathic pain symptoms confirmed by evaluating mechanical hyperalgesia (Randall and Selitto test and allodynia (von Frey hair test. Control animals did not present any alteration (sham-animals. The selective inhibitor of nNOS, 7-nitroindazole (0.2 and 2 µg in 50 µL, blocked hyperalgesia and allodynia induced by SNT. Immunohistochemical analysis showed that nNOS was increased (48% by day 30 in the lumbar spinal cord after SNT. This increase was observed near the central canal (Rexed’s lamina X and also in lamina I-IV of the dorsal horn. Real-time PCR results indicated an increase of nNOS mRNA detected from 1 to 30 days after SNT, with the highest increase observed 1 day after injury (1469%. Immunoblotting confirmed the increase of nNOS in the spinal cord between 1 and 15 days post-lesion (20%, reaching the greatest increase (60% 30 days after surgery. The present findings demonstrate an increase of nNOS after peripheral nerve injury that may contribute to the increase of NO production observed after peripheral neuropathy.

  17. Changes in Microbial Nitrogen Dynamics with Soil Depth, and along a Latitudinal Transect in Western Siberia

    Science.gov (United States)

    Wild, B.; Schnecker, J.; Knoltsch, A.; Takriti, M.; Mooshammer, M.; Gentsch, N.; Mikutta, R.; Alves, R.; Gittel, A.; Lashchinskiy, N.; Richter, A.

    2015-12-01

    Plant productivity is often limited by low N availability, and this has been attributed to the slow breakdown of N-containing polymers such as proteins into amino acids that are small enough for uptake. Under such conditions, plants and microorganisms efficiently use the available N for growth, and the microbial release of excess N as ammonium (N mineralization), as well as the transformation of ammonium into nitrate (nitrification) is low. Nitrogen limitation is expected to increase towards high latitudes as conditions become less favourable for decomposition. On the other hand, within an ecosystem, microbial N limitation is expected to decrease with soil depth, following the decrease in the C/N ratio of organic matter. To test these hypotheses, we sampled organic topsoils, mineral topsoils and mineral subsoils from seven ecosystems along a latitudinal transect in Western Siberia, ranging from tundra (67°N) to boreal forest and further to steppe (54°N), and determined gross rates of protein depolymerization, N mineralization and nitrification using 15N pool dilution assays. We found that all rates decreased with depth following the decrease in organic matter content. Related to microbial biomass, however, only protein depolymerization decreased with depth, whereas N mineralization and nitrification significantly increased. This pattern was consistent across the seven ecosystems studied. Furthermore, we did not find indications for a decrease in microbial N limitation from arctic to temperate systems. Our findings thus challenge the perception of ubiquitous N limitation at high latitudes, but suggest a transition from N to C limitation of microorganisms with soil depth. With microbial N immobilization constrained by low C availability, subsoils might harbour an easily available N pool that can contribute to plant N nutrition, but might also promote N losses from the ecosystem, e.g., by nitrate leaching, even in high latitude systems such as tundra and boreal

  18. Therapy induces widespread reorganization of motor cortex after complete spinal transection that supports motor recovery.

    Science.gov (United States)

    Ganzer, Patrick D; Manohar, Anitha; Shumsky, Jed S; Moxon, Karen A

    2016-05-01

    Reorganization of the somatosensory system and its relationship to functional recovery after spinal cord injury (SCI) has been well studied. However, little is known about the impact of SCI on organization of the motor system. Recent studies suggest that step-training paradigms in combination with spinal stimulation, either electrically or through pharmacology, are more effective than step training alone at inducing recovery and that reorganization of descending corticospinal circuits is necessary. However, simpler, passive exercise combined with pharmacotherapy has also shown functional improvement after SCI and reorganization of, at least, the sensory cortex. In this study we assessed the effect of passive exercise and serotonergic (5-HT) pharmacological therapies on behavioral recovery and organization of the motor cortex. We compared the effects of passive hindlimb bike exercise to bike exercise combined with daily injections of 5-HT agonists in a rat model of complete mid-thoracic transection. 5-HT pharmacotherapy combined with bike exercise allowed the animals to achieve unassisted weight support in the open field. This combination of therapies also produced extensive expansion of the axial trunk motor cortex into the deafferented hindlimb motor cortex and, surprisingly, reorganization within the caudal and even the rostral forelimb motor cortex areas. The extent of the axial trunk expansion was correlated to improvement in behavioral recovery of hindlimbs during open field locomotion, including weight support. From a translational perspective, these data suggest a rationale for developing and optimizing cost-effective, non-invasive, pharmacological and passive exercise regimes to promote plasticity that supports restoration of movement after spinal cord injury.

  19. Line Transect and Triangulation Surveys Provide Reliable Estimates of the Density of Kloss' Gibbons (Hylobates klossii) on Siberut Island, Indonesia.

    Science.gov (United States)

    Höing, Andrea; Quinten, Marcel C; Indrawati, Yohana Maria; Cheyne, Susan M; Waltert, Matthias

    2013-02-01

    Estimating population densities of key species is crucial for many conservation programs. Density estimates provide baseline data and enable monitoring of population size. Several different survey methods are available, and the choice of method depends on the species and study aims. Few studies have compared the accuracy and efficiency of different survey methods for large mammals, particularly for primates. Here we compare estimates of density and abundance of Kloss' gibbons (Hylobates klossii) using two of the most common survey methods: line transect distance sampling and triangulation. Line transect surveys (survey effort: 155.5 km) produced a total of 101 auditory and visual encounters and a density estimate of 5.5 gibbon clusters (groups or subgroups of primate social units)/km(2). Triangulation conducted from 12 listening posts during the same period revealed a similar density estimate of 5.0 clusters/km(2). Coefficients of variation of cluster density estimates were slightly higher from triangulation (0.24) than from line transects (0.17), resulting in a lack of precision in detecting changes in cluster densities of triangulation and triangulation method also may be appropriate.

  20. Erythropoietin improves place learning in fimbria-fornix-transected rats and modifies the search pattern of normal rats

    DEFF Research Database (Denmark)

    Mogensen, Jesper; Miskowiak, Kamilla; Sørensen, Thomas Alrik;

    2004-01-01

    The acquisition of a water-maze-based allocentric place learning task was studied in four groups of rats: two groups subjected to bilateral transections of the fimbria-fornix and two groups undergoing a sham control operation. At the moment of surgery all animals were given one systemic (intraper......The acquisition of a water-maze-based allocentric place learning task was studied in four groups of rats: two groups subjected to bilateral transections of the fimbria-fornix and two groups undergoing a sham control operation. At the moment of surgery all animals were given one systemic......-associated impairment. The two sham-operated groups did not differ with respect to the proficiency of task acquisition. But administration of EPO to intact animals caused a significant modification of swim patterns-apparently reflecting a somewhat modified strategy of task solution. It is concluded that systemic...... administration of EPO significantly improves the posttraumatic functional recovery of the presently studied place learning task after transections of the fimbria-fornix. Additionally, administration of EPO influences the strategy, although not quality, of task solution in normal (sham-operated) rats....

  1. Thoracic 9 Spinal Transection-Induced Model of Muscle Spasticity in the Rat: A Systematic Electrophysiological and Histopathological Characterization

    Science.gov (United States)

    Corleto, Jose A.; Bravo-Hernández, Mariana; Kamizato, Kota; Kakinohana, Osamu; Santucci, Camila; Navarro, Michael R.; Platoshyn, Oleksandr; Cizkova, Dasa; Lukacova, Nadezda; Taylor, Julian; Marsala, Martin

    2015-01-01

    The development of spinal hyper-reflexia as part of the spasticity syndrome represents one of the major complications associated with chronic spinal traumatic injury (SCI). The primary mechanism leading to progressive appearance of muscle spasticity is multimodal and may include loss of descending inhibitory tone, alteration of segmental interneuron-mediated inhibition and/or increased reflex activity to sensory input. Here, we characterized a chronic thoracic (Th 9) complete transection model of muscle spasticity in Sprague-Dawley (SD) rats. Isoflurane-anesthetized rats received a Th9 laminectomy and the spinal cord was transected using a scalpel blade. After the transection the presence of muscle spasticity quantified as stretch and cutaneous hyper-reflexia was identified and quantified as time-dependent changes in: i) ankle-rotation-evoked peripheral muscle resistance (PMR) and corresponding electromyography (EMG) activity, ii) Hoffmann reflex, and iii) EMG responses in gastrocnemius muscle after paw tactile stimulation for up to 8 months after injury. To validate the clinical relevance of this model, the treatment potency after systemic treatment with the clinically established anti-spastic agents baclofen (GABAB receptor agonist), tizanidine (α2-adrenergic agonist) and NGX424 (AMPA receptor antagonist) was also tested. During the first 3 months post spinal transection, a progressive increase in ankle rotation-evoked muscle resistance, Hoffmann reflex amplitude and increased EMG responses to peripherally applied tactile stimuli were consistently measured. These changes, indicative of the spasticity syndrome, then remained relatively stable for up to 8 months post injury. Systemic treatment with baclofen, tizanidine and NGX424 led to a significant but transient suppression of spinal hyper-reflexia. These data demonstrate that a chronic Th9 spinal transection model in adult SD rat represents a reliable experimental platform to be used in studying the

  2. Thoracic 9 Spinal Transection-Induced Model of Muscle Spasticity in the Rat: A Systematic Electrophysiological and Histopathological Characterization.

    Directory of Open Access Journals (Sweden)

    Jose A Corleto

    Full Text Available The development of spinal hyper-reflexia as part of the spasticity syndrome represents one of the major complications associated with chronic spinal traumatic injury (SCI. The primary mechanism leading to progressive appearance of muscle spasticity is multimodal and may include loss of descending inhibitory tone, alteration of segmental interneuron-mediated inhibition and/or increased reflex activity to sensory input. Here, we characterized a chronic thoracic (Th 9 complete transection model of muscle spasticity in Sprague-Dawley (SD rats. Isoflurane-anesthetized rats received a Th9 laminectomy and the spinal cord was transected using a scalpel blade. After the transection the presence of muscle spasticity quantified as stretch and cutaneous hyper-reflexia was identified and quantified as time-dependent changes in: i ankle-rotation-evoked peripheral muscle resistance (PMR and corresponding electromyography (EMG activity, ii Hoffmann reflex, and iii EMG responses in gastrocnemius muscle after paw tactile stimulation for up to 8 months after injury. To validate the clinical relevance of this model, the treatment potency after systemic treatment with the clinically established anti-spastic agents baclofen (GABAB receptor agonist, tizanidine (α2-adrenergic agonist and NGX424 (AMPA receptor antagonist was also tested. During the first 3 months post spinal transection, a progressive increase in ankle rotation-evoked muscle resistance, Hoffmann reflex amplitude and increased EMG responses to peripherally applied tactile stimuli were consistently measured. These changes, indicative of the spasticity syndrome, then remained relatively stable for up to 8 months post injury. Systemic treatment with baclofen, tizanidine and NGX424 led to a significant but transient suppression of spinal hyper-reflexia. These data demonstrate that a chronic Th9 spinal transection model in adult SD rat represents a reliable experimental platform to be used in studying the

  3. Thoracic 9 Spinal Transection-Induced Model of Muscle Spasticity in the Rat: A Systematic Electrophysiological and Histopathological Characterization.

    Science.gov (United States)

    Corleto, Jose A; Bravo-Hernández, Mariana; Kamizato, Kota; Kakinohana, Osamu; Santucci, Camila; Navarro, Michael R; Platoshyn, Oleksandr; Cizkova, Dasa; Lukacova, Nadezda; Taylor, Julian; Marsala, Martin

    2015-01-01

    The development of spinal hyper-reflexia as part of the spasticity syndrome represents one of the major complications associated with chronic spinal traumatic injury (SCI). The primary mechanism leading to progressive appearance of muscle spasticity is multimodal and may include loss of descending inhibitory tone, alteration of segmental interneuron-mediated inhibition and/or increased reflex activity to sensory input. Here, we characterized a chronic thoracic (Th 9) complete transection model of muscle spasticity in Sprague-Dawley (SD) rats. Isoflurane-anesthetized rats received a Th9 laminectomy and the spinal cord was transected using a scalpel blade. After the transection the presence of muscle spasticity quantified as stretch and cutaneous hyper-reflexia was identified and quantified as time-dependent changes in: i) ankle-rotation-evoked peripheral muscle resistance (PMR) and corresponding electromyography (EMG) activity, ii) Hoffmann reflex, and iii) EMG responses in gastrocnemius muscle after paw tactile stimulation for up to 8 months after injury. To validate the clinical relevance of this model, the treatment potency after systemic treatment with the clinically established anti-spastic agents baclofen (GABAB receptor agonist), tizanidine (α2-adrenergic agonist) and NGX424 (AMPA receptor antagonist) was also tested. During the first 3 months post spinal transection, a progressive increase in ankle rotation-evoked muscle resistance, Hoffmann reflex amplitude and increased EMG responses to peripherally applied tactile stimuli were consistently measured. These changes, indicative of the spasticity syndrome, then remained relatively stable for up to 8 months post injury. Systemic treatment with baclofen, tizanidine and NGX424 led to a significant but transient suppression of spinal hyper-reflexia. These data demonstrate that a chronic Th9 spinal transection model in adult SD rat represents a reliable experimental platform to be used in studying the

  4. NOAA Line Shapefile- Locations of Phantom S2 ROV Underwater Video Transects, US Virgin Islands, Project NF-05-05, 2005, UTM 20N WGS84

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This dataset contains a line shapefile showing the trackline of various Remotely Operated Vehicle (ROV) underwater video transects in the US Virgin Islands.NOAA's...

  5. National Coral Reef Monitoring Program: Assessment of coral reef communities in Flower Garden Banks National Marine Sanctuary using the Belt Transect fish census method

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Belt Transect method is used to conduct fish surveys at Flower Garden Banks National Marine Sanctuary (FGBNMS) in the Gulf of Mexico as part of the National...

  6. GPS data collected for transects as part of the pre-construction survey at Emergent Sandbar Habitat site on the Missouri River near river mile 769.8

    Data.gov (United States)

    U.S. Geological Survey, Department of the Interior — This data set contains land surface elevations on dry and wadeable portions of transects for the hydrographic surveys on the Missouri River below Gavins Point Dam...

  7. Postconstruction water velocity arrays collected at selected transects on the Missouri River downstream from Gavins Point Dam near River Mile 769.8

    Data.gov (United States)

    U.S. Geological Survey, Department of the Interior — This data set contains arrays of water velocity collected on selected transects of the Missouri River below Gavin's Point Dam near River Mile 769.8.

  8. Fishery Biology and Stock Assessment Division (FBSAD) Recruit Reef Fish Belt Transect and Habitat Quadrat Surveys at Hawaii Island (Big Island), Main Hawaiian Islands, 2005 (NODC Accession 0046935)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Shore-based belt transects were conducted at 8-13 m depths at 3 longshore sites on the leeward coast (North and South Kohala districts) of the Big Island (Hawaii...

  9. NOAA Polyline Shapefile - ROV transect Lines, US Caribbean - North East Reserve, Puerto Rico - M-I907-NF-13 (2013), UTM 20N NAD83

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This shapefile denotes the transect lines of the underwater video and photos that were collected by NOAA scientists using a Spectrum Phantom S2 ROV (remotely...

  10. NOAA Polyline Shapefile - ROV transect Points, US Caribbean - North East Reserve, Puerto Rico - M-I907-NF-13 (2013), UTM 20N NAD83

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This shapefile denotes the transect points of the underwater video and photos that were collected by NOAA scientists using a Spectrum Phantom S2 ROV (remotely...

  11. Map images portraying flight paths of low-altitude transects over the Arctic Network of national park units and Selawik National Wildlife Refuge, Alaska, July 2013

    Data.gov (United States)

    U.S. Geological Survey, Department of the Interior — Maps portraying the flight paths for low altitude transects conducted from small aircraft over the National Park Service’s Arctic Network (Bering Land Bridge...

  12. National Coral Reef Monitoring Program: Assessment of coral reef communities in U.S. Virgin Islands (USVI) using the Belt Transect fish census method

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Belt Transect method is used to conduct fish surveys in the U.S. Virgin Islands (USVI) as part of the National Coral Reef Monitoring Program (NCRMP). The Belt...

  13. NOAA Polyline Shapefile - ROV transect points, US Caribbean - North East Reserve, Puerto Rico - M-I907-NF-12 (2012), UTM 20N NAD83 (NCEI Accession 0131856)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This shapefile denotes the transect points of the underwater video and photos that were collected by NOAA scientists using a Spectrum Phantom S2 ROV (remotely...

  14. Evaluating the use of the belt transect method in determining native plant composition changes in upland and wet meadow habitats on Lacreek National Wildlife Refuge

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — Determine if the belt transect method can be used to detect a 10% increase in native plant composition on upland and wet meadow habitats on Lacreek NWR within a 10...

  15. Hawaii Coral Reef Assessment and Monitoring Program (CRAMP): Digital Still Images from Transects on Maui, Molokai, and Kauai, 2007 (NODC Accession 0056791)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This dataset consists of digital still transect images (JPG files) from CRAMP surveys taken in 2007 from 9 sites on Maui, 3 sites on Molokai, and 1 site on Kauai....

  16. Hawaii Coral Reef Assessment and Monitoring Program (CRAMP): digital still images from transects on Maui, Molokai, and Kauai, 2007 (NODC Accession 0056791)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This dataset consists of digital still transect images (JPG files) from CRAMP surveys taken in 2007 from 9 sites on Maui, 3 sites on Molokai, and 1 site on Kauai....

  17. GPS data collected for transects as part of the post-construction survey at Emergent Sandbar Habitat site on the Missouri River near river mile 769.8

    Data.gov (United States)

    U.S. Geological Survey, Department of the Interior — This data set contains land surface elevations on dry and wadeable portions of transects for the hydrographic surveys on the Missouri River below Gavins Point Dam...

  18. NOAA Polyline Shapefile - ROV transect Lines, US Caribbean - North East Reserve, Puerto Rico - M-I907-NF-12 (2012), UTM 20N NAD83 (NCEI Accession 0131856)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This shapefile denotes the transect lines of the underwater video and photos that were collected by NOAA scientists using a Spectrum Phantom S2 ROV (remotely...

  19. Multi-sensor synthesis of vegetation pattern over a large climatic gradient transect in Africa

    Science.gov (United States)

    Guan, K.; Wood, E. F.; Caylor, K. K.

    2010-12-01

    African savannas are highly susceptible to degradation by drought, changes in fire regimes, and overgrazing. These degradations threaten important ecosystem services (e.g., forage, freshwater and food supply) and socio-economic development. The vast terrestrial coverage of savannas makes the effect of structural changes on global water and carbon cycles potentially significant, though uncertain. Vegetation composition (i.e. tree/grass fractions) is a key variable in characterizing savanna ecosystems. Accurately quantifying the fractions of trees, grasses and bare soil in savannas is essential for understanding ecosystem response to climate variability and assessing ecosystem resilience to possible degradations. In this research, we applied the `mean-sensitivity unmixing method' over a large climatic gradient transect in eastern Africa. We comprehensively assessed the applicability of deriving vegetation fractions from three independent remote sensing datasets: (i) normalized differential vegetation index (NDVI); (ii) scatterometer backscatter coefficients (dB); and (iii) vegetation optical depth (VOD). We focused on long-term average, interannual sensitivity to precipitation, and physical interpretation of these three datasets. We found that NDVI and VOD were highly correlated for long-term average; while dB showed abnormally high value for pixels with more bare soil. Three datasets showed similar interannual senstivity to precipitation for each landcover type, that grassland had the highest sensitivity, and both woodland and bare soil had lower sensitivity. Nevertheless, microwave-based dB and VOD largely overcame cloudiness problem found in NDVI, and showed more robust responses to precipitation. Furthermore, the lag times of the maximum response of three vegetation datasets to precipitation peak time were different, suggesting three datasets may capture distinct components of vegetation information and phenology. We proposed a synergic use of NDVI and dB, and

  20. Substrate use efficiency of microbial communities along a latitudinal transect through Western Siberia

    Science.gov (United States)

    Takriti, Mounir; Wild, Birgit; Schnecker, Jörg; Mooshammer, Maria; Knoltsch, Anna; Lashchinskiy, Nikolay; Eloy Alves, Ricardo J.; Gentsch, Norman; Gittel, Antje; Mikutta, Robert; Wanek, Wolfgang; Richter, Andreas

    2014-05-01

    As stable soil organic matter is largely derived from microbial compounds, the partitioning of C uptake by microorganisms into growth and respiration determines the C storage potential in soils. The proportion of substrate carbon (C) which is invested into new microbial biomass (i.e., microbial growth), compared to the fraction of substrate carbon C which is respired as as CO2,is often referred to as substrate use efficiency (SUE) or carbon use efficiency (CUE). According to stoichiometric theory, the CUE of microbes is strongly controlled by the availability of nutrients such as nitrogen (N), as microorganisms have to maintain their biomass stoichiometry within relatively narrow boundaries. Hence, when microorganisms are nitrogen limited, excess C is respired (low SUE), while conversely excess N is mineralized when C is limiting (high SUE). In this study we took advantage of the high variability in biotic and abiotic factors, such as C:N ratio and litter input quality, between samples taken from the top three soil horizons (organic topsoil, upper and lower mineral horizon) from six different ecosystems along a 1,500 km linear-distance latitudinal transect through Western Siberia. We hypothesized that SUE would increase with soil depth, as organic matter becomes successively enriched with N relative to C, and decrease with latitude, as ecosystem N availability decreases. To determine SUE we measured uptake and respiration of a mixture of 13C labeled substrate (consisting of monosaccharides, organic acids, amino acids and amino sugars), as well as C and N pools and extracellular enzyme activities. In contrast to our expectations, we found that microbial SUE in lower mineral horizons was significantly lower than in upper mineral horizons, while there was no significant difference to the organic horizons. This is contradictory to the theory of ecological stoichiometry, since microbial SUE did not increase with decreasing soil C:N. Potential oxidative enzyme activities

  1. In vivo longitudinal Myelin Water Imaging in rat spinal cord following dorsal column transection injury.

    Science.gov (United States)

    Kozlowski, Piotr; Rosicka, Paulina; Liu, Jie; Yung, Andrew C; Tetzlaff, Wolfram

    2014-04-01

    Longitudinal Myelin Water Imaging was carried out in vivo to characterize white matter damage following dorsal column transection (DC Tx) injury at the lumbar level L1 of rat spinal cords. A transmit-receive implantable coil system was used to acquire multiple spin-echo (MSE) quantitative T2 data from the lumbar spinal cords of 16 rats at one week pre-injury as well as 3 and 8weeks post-injury (117 microns in-plane resolution and 1.5mm slice thickness). In addition, ex vivo MSE and DTI data were acquired from cords fixed and excised at 3 or 8weeks post injury using a solenoid coil. The MSE data were used to generate Myelin Water Fractions (MWFs) as a surrogate measure of myelin content, while DTI data were acquired to study damage to the axons. Myelin damage was assessed histologically with Eriochrome cyanine (EC) and Myelin Basic Protein in degenerated myelin (dgen-MBP) staining, and axonal damage was assessed by neurofilament-H in combination with neuron specific beta-III-tubulin (NF/Tub) staining. These MRI and histological measures of injury were studied in the dorsal column at 5mm cranial and 5mm caudal to injury epicenter. MWF increased significantly at 3weeks post-injury at both the cranial and caudal sites, relative to baseline. The values on the cranial side of injury returned to baseline at 8weeks post-injury but remained elevated on the caudal side. This trend was found in both in vivo and ex vivo data. This MWF increase was likely due to the presence of myelin debris, which were cleared by 8 weeks on the cranial, but not the caudal, side. Both EC and dgen-MBP stains displayed similar trends. MWF showed significant correlation with EC staining (R=0.63, p=0.005 in vivo and R=0.74, p=0.0001 ex vivo). MWF also correlated strongly with the dgen-MBP stain, but only on the cranial side (R=0.64, p=0.05 in vivo; R=0.63, p=0.038 ex vivo). This study demonstrates that longitudinal MWI in vivo can accurately characterize white matter damage in DC Tx model of injury

  2. Electrospun micro- and nanofiber tubes for functional nervous regeneration in sciatic nerve transections

    Directory of Open Access Journals (Sweden)

    Amadio Stefano

    2008-04-01

    Full Text Available Abstract Background Although many nerve prostheses have been proposed in recent years, in the case of consistent loss of nervous tissue peripheral nerve injury is still a traumatic pathology that may impair patient's movements by interrupting his motor-sensory pathways. In the last few decades tissue engineering has opened the door to new approaches;: however most of them make use of rigid channel guides that may cause cell loss due to the lack of physiological local stresses exerted over the nervous tissue during patient's movement. Electrospinning technique makes it possible to spin microfiber and nanofiber flexible tubular scaffolds composed of a number of natural and synthetic components, showing high porosity and remarkable surface/volume ratio. Results In this study we used electrospun tubes made of biodegradable polymers (a blend of PLGA/PCL to regenerate a 10-mm nerve gap in a rat sciatic nerve in vivo. Experimental groups comprise lesioned animals (control group and lesioned animals subjected to guide conduits implantated at the severed nerve stumps, where the tubular scaffolds are filled with saline solution. Four months after surgery, sciatic nerves failed to reconnect the two stumps of transected nerves in the control animal group. In most of the treated animals the electrospun tubes induced nervous regeneration and functional reconnection of the two severed sciatic nerve tracts. Myelination and collagen IV deposition have been detected in concurrence with regenerated fibers. No significant inflammatory response has been found. Neural tracers revealed the re-establishment of functional neuronal connections and evoked potential results showed the reinnervation of the target muscles in the majority of the treated animals. Conclusion Corroborating previous works, this study indicates that electrospun tubes, with no additional biological coating or drug loading treatment, are promising scaffolds for functional nervous regeneration. They

  3. Production of dissolved organic carbon in forest soils along the north-south European transect

    International Nuclear Information System (INIS)

    The aim of this study is to estimate the C loss from forest soils due to the production of dissolved organic C (DOC) along a north-south European transect. Dissolved organic matter (DOM) was extracted from the forest soils incubated at a controlled temperature and water content. Soils were sampled from forest plots from Sweden to Italy. The plots represent monocultures of spruce, pine and beech and three selected chronosequences of spruce and beech spanning a range of mean annual temperature from 2 to 14 deg. C. The DOM was characterized by its DOC/DON ratio and the C isotope composition δ13C. The DOC/DON ratio of DOM varied from 25 to 15 after 16 days of incubation and it decreased to between 16 and 10 after 126 days. At the beginning of incubation the δ13C values of DOC were 1 per mille or 2 per mille less negative than incubated soils. At the end of the experiment δ13C of DOC were the same as soil values. In addition to DOC production heterotrophic respiration and N mineralization were measured on the incubated soils. The DON production rates decreased from 30 to 5 μgN gC-1 d-1 after 16 days of incubation to constant values from 5 to 2 μgN gC-1 d-1 after 126 days at the end of experiment. The DIN production rates were nearly constant during the experiments with values ranging from 20 to 4 μgN gC-1 d-1. DOC production followed first-order reaction kinetics and heterotrophic respiration followed zero-order reaction kinetics. Kinetic analysis of the experimental data yielded mean annual DOC and respiration productions with respect to sites. Mean annual estimates of DOC flux varied from 3 to 29 g of C m-2 (1-19 mg C g-1 of available C), corresponding to mean DOC concentrations from 2 to 85 mg C L-1.

  4. Linking water balance of mountain grasslands along altitudinal transects to climate and land-use change

    Science.gov (United States)

    Leitinger, Georg; Obojes, Nikolaus; Tasser, Erich; Tappeiner, Ulrike

    2010-05-01

    Changes of the water balance of mountain grasslands with regard to climate and land-use changes are a popular research field since years. Measuring evapotranspiration (EVT) for different land-use types and plant communities at varying sea level helps us to understand the change of water availability in a future environment. Linked with transplantation experiments, this method is promising to cover most forecasted scenarios. Although the mentioned approach is well established, our study is innovative in so far as the field work as well as data analyses was supported by more than 50 pupils from a secondary school for agriculture and food industry. Hence, a huge number of field measurements could be conducted at the same time distributed over a whole alpine valley. In our study site Stubai Valley (300km²), Tyrol, Austria, 13 sites on 4 different altitudinal transects (valley bottom, hillside, and sub-alpine/alpine) ranging from 900m a.s.l. up to 2400m a.s.l. were selected and equipped with weather stations recording air temperature, air humidity, precipitation, solar radiation, and soil water content in different soil depths at 15-minute interval. Additionally, more than 300 small lysimeters have been installed and data on EVT, infiltration, leaf conductivity, and soil wetness was collected on 7 measuring days. The measurements spanned an entire daylight period from sunrise to sunset. Moreover, soil and vegetation analyses on all selected plots complete the enormous data pool. The lysimeters on each plot contained samples of long-stemmed local vegetation (1 cut / 1 uncut), short-stemmed local vegetation (1 cut / 1 uncut), alpine standard vegetation (1), intensive standard vegetation (1 cut / 1 uncut), and water for potential transpiration (1). Each type was replicated three times resulting in a total number of 24 lysimeters per study plot. Results revealed a little increase in EVT rates for the Alpine Standard Vegetation transplanted to lower altitudes and slight

  5. Dissolved Organic Matter Along a North South Transect of the Yenisei River

    Science.gov (United States)

    Roth, V.; Dittmar, T.; Gaupp, R.; Gleixner, G.

    2012-12-01

    The molecular composition of dissolved organic matter (DOM) contains important information on properties and processes like decomposition in terrestrial and aquatic systems. To explore the source characteristics of DOM in relation to environmental factors, tributaries and bogs along the Yenisei River between 56° N near Krasnoyarsk and 68° N at Khantayka River were sampled. This includes a transect of 1000 km across a climatic gradient with different vegetation zones and permafrost influence. The sampling took place in July and August 1998 during the one-month BOBCAT (Boat on a Boreal Carbon Tour) campaign of the Max Planck Institute for Biogeochemistry (Jena, Germany) and the Institute of Forest (Krasnoyarsk, Russia). After filtration with GF/F, the samples were freeze-dried for following analysis. To unravel the complexity of DOM in our samples on a molecular basis, we applied Electrospray Ionization Fourier-Transform Ion Cyclotron Resonance Mass Spectrometry (ESI-FT-ICR-MS). For ESI-FT-ICR-MS freeze-dried material was re-dissolved and extracted by solid phase extraction (SPE) with Varian Bond Elute PPL according to Dittmar et al. 2008. The desalted samples were measured in negative ionization mode at the Bruker Solarix 15 T FT-ICR-MS at the University of Oldenburg (Germany). The data of the highly resolved and accurately calibrated masses was used to calculate the corresponding molecular formulas. Different multivariate statistical tools, like principal component analysis (PCA) in combination with environmental parameters, and graphical display methods are applied to the data to explore differences and similarities between samples and as well possible correlations with environmental parameters. In our contribution we show the results of our comparative study. Major outcomes are the correlation between molecular composition of DOM and environmental parameters like DOC concentration, pH and geographical latitude. We found, that the pH in water is a driving factor

  6. Modelling soil organic carbon stocks along topographic transects under climate change scenarios using CarboSOIL

    Science.gov (United States)

    Kotb Abd-Elmabod, Sameh; Muñoz-Rojas, Miriam; Jordán, Antonio; Anaya-Romero, María; de la Rosa, Diego

    2014-05-01

    CarboSOIL is a land evaluation model for soil organic carbon (SOC) accounting under global change scenarios (Muñoz-Rojas et al., 2013a; 2013b) and is a new component of the MicroLEIS Decision Support System. MicroLEIS is a tool for decision-makers dealing with specific agro-ecological problems as, for example, soil contamination risks (Abd-Elmabod et al., 2010; Abd-Elmabod et al., 2012)which has been designed as a knowledge-based approach incorporating a set of interlinked data bases. Global change and land use changes in recent decades have caused relevant impacts in vegetation carbon stocks (Muñoz-Rojas et al., 2011) and soil organic carbon stocks, especially in sensible areas as the Mediterranean region (Muñoz-Rojas et al., 2012a; 2012b). This study aims to investigate the influence of topography, climate, land use and soil factors on SOC stocks by the application of CarboSOIL in a representative area of the Mediterranean region (Seville, Spain). Two topographic transects (S-N and W-E oriented) were considered, including 63 points separated 4 km each. These points are associated to 41 soil profiles extracted from the SDBm soil data base (De la Rosa et al., 2001) and climatic information (average minimum temperature, average maximum temperature and average rainfall per month) extracted from raster data bases (Andalusian Environmental Information Network, REDIAM). CarboSOIL has been applied along topographic transects at different soil depths and under different climate change scenarios. Climate scenarios have been calculated according to the global climate model (CNRMCM3) by extracting spatial climate data under IPCC A1B scenario for the current period (average data from 1960-2000), 2040, 2070 and 2100. In the current scenario, results show that the highest SOC stock values located on Typic Haploxeralfs under olive groves for soil sections 0-25 cm and for 25-50 cm, but the highest values were determined on fruit-cropped Rendolic Xerothent in the 50-75cm

  7. Spatial Variation in Microbial Populations Across a Cattle Feedlot Transect from Barn to Retention Basin

    Science.gov (United States)

    Run-off from livestock production can pose a risk to environmental quality particularly in karst terrains where geomorphic features like sinkholes provide rapid passage for contaminants to ground water resources. In these environments, it is especially important to understand how variations in terr...

  8. Positive effects of bFGF modified rat amniotic epithelial cells transplantation on transected rat optic nerve.

    Directory of Open Access Journals (Sweden)

    Jia-Xin Xie

    Full Text Available Effective therapy for visual loss caused by optic nerve injury or diseases has not been achieved even though the optic nerve has the regeneration potential after injury. This study was designed to modify amniotic epithelial cells (AECs with basic fibroblast growth factor (bFGF gene, preliminarily investigating its effect on transected optic nerve.A human bFGF gene segment was delivered into rat AECs (AECs/hbFGF by lentiviral vector, and the gene expression was examined by RT-PCR and ELISA. The AECs/hbFGF and untransfected rat AECs were transplanted into the transected site of the rat optic nerve. At 28 days post transplantation, the survival and migration of the transplanted cells was observed by tracking labeled cells; meanwhile retinal ganglion cells (RGCs were observed and counted by employing biotin dextran amine (BDA and Nissl staining. Furthermore, the expression of growth associated protein 43 (GAP-43 within the injury site was examined with immunohistochemical staining.The AECs/hbFGF was proven to express bFGF gene and secrete bFGF peptide. Both AECs/hbFGF and AECs could survive and migrate after transplantation. RGCs counting implicated that RGCs numbers of the cell transplantation groups were significantly higher than that of the control group, and the AECs/hbFGF group was significantly higher than that of the AECs group. Moreover GAP-43 integral optical density value in the control group was significantly lower than that of the cell transplantation groups, and the value in the AECs/hbFGF group was significantly higher than that of the AECs group.AECs modified with bFGF could reduce RGCs loss and promote expression of GAP-43 in the rat optic nerve transected model, facilitating the process of neural restoration following injury.

  9. Assessing Fractional Tree, Grass, and Bare Soil Cover from NDVI and Rainfall Time Series along the Kalahari Transect, Africa

    Science.gov (United States)

    Scanlon, T. M.; Albertson, J. D.; Caylor, K. K.; Williams, C. A.

    2001-12-01

    Savanna ecosystems are water-limited, a characteristic that can be exploited to estimate fractional cover of trees, grass, and bare soil over large-scale areas from synthesis of remote sensing and rainfall measurements. A method is presented to estimate fractional cover components along the Kalahari Transect (KT), an aridity gradient in southern Africa, based upon the differing ways in which grasses and trees respond to rainfall. Mean wet season normalized difference vegetation index (NDVI) and the sensitivity of the NDVI to variation in wet season rainfall are used as input into a linear unmixing model, and end-members for this analysis are extracted on the basis of best fit to the observed data. The end-members for the mixing analysis are consistent with the qualitative characteristics of trees (high NDVI, low sensitivity of NDVI to interannual variations in rainfall), bare soil (low NDVI, low sensitivity), and the transient grass/ bare soil area (moderate NDVI, high sensitivity). Sensitivity of NDVI to rainfall was based upon the relationship between NDVI and the standardized anomalies, or z-score, of the wet season precipitation, resulting in a 22% improvement in the number of significant relationships (parea is limited by the tree fractional cover on the wetter end of the transect, peaks at approximately 450 mm of mean wet season rainfall, and is limited by rainfall on the more arid portion of the transect. With NDVI for grass inferred from the data, predictions of yearly tree, grass, and bare soil fractional cover can be derived. No calibration or training sets were required for this unmixing procedure, and an additional advantage of this method over traditional unmixing approaches is that cover components can be predicted for future rainfall scenarios. This remote sensing-based model framework, together with a tree/grass interaction submodel, could be used to predict long-term migration of the cover components along this gradient in response to climate

  10. Controls on compound specific 2H/1H of leaf waxes along a North American monsoonal transect

    Science.gov (United States)

    Berke, M. A.; Tipple, B. J.; Hambach, B.; Ehleringer, J. R.

    2013-12-01

    The use of hydrogen isotope ratios of sedimentary n-alkanes from leaf waxes has become an important method for the reconstruction of paleohydrologic conditions. Ideally, the relationship between lipid 2H/1H values and source water is one-to-one. But the extent to which the 2H/1H values are altered between initial source water and lipid 2H/1H values varies by plant type and environment. Additionally, these variables may be confounded by use of varied source waters by plants in the same ecosystem. Here, we use a transect study across the arid southwestern landscape of the United States, which is heavily influenced by the North American Monsoon, to study the variability in 2H/1H values of leaf waxes in co-occurring plants from Tucson, Arizona to Salt Lake City, Utah. Perennials, including rabbit brush (Chrysothamnus nauseosus), sagebrush (Artemisia tridentata), and gambel oak (Quercus gambelii) and an annual plant, sunflower (Helianthus annuus), were chosen for their wide geographic distribution along the entire transect. Our results indicate that n-alkane distribution for each plant was similar and generally showed no relationship to environmental variables (elevation, mean annual precipitation, latitude, and temperature). However, we find evidence of n-alkane 2H/1H value relating to transect latitude, a relationship that is weaker for all samples combined than the strong individual correlation for each plant species. Further, these 2H/1H values suggest that not all plants in the monsoon region utilize monsoon-delivered precipitation. These results imply an adaptation to discontinuous spatial coverage and amount of monsoonal precipitation and suggest care must be taken when assuming consistent source water for different plants, particularly in regions with highly seasonal precipitation delivery.

  11. Ground and surface water quality along a dambo transect in Chihota smallholder farming area, Marondera district, Zimbabwe

    Science.gov (United States)

    Wuta, M.; Nyamadzawo, G.; Mlambo, J.; Nyamugafata, P.

    2016-04-01

    In many smallholder farms in sub-Saharan Africa dambos are used for grazing and crop production especially horticultural crops. Increased use of dambos especially for crop production can result in ground and surface water pollution. Ground and surface water quality along a dambo transect in Chihota, Zimbabwe, was investigated between October 2013 and February 2014. The transect was divided into; upland (control), dambo gardens (mid-slope) and the river (valley bottom). Water samples for quality assessment were collected in October 2013 (peak of dry season) and February 2014 (peak of rainy season). The collected water samples were analysed for pH, faecal coliforms, total nitrogen, electrical conductivity, total dissolved solids (TDS), and some selected nutrients (P, K, Ca, Mg, Na, Zn, and Cu). Water pH was 7.0, 6.4 and 6.1 for river water, garden and upland wells respectively. During the wet season total nitrogen (TN) concentrations were 233 mg/L for uplands, 242 mg/L for gardens and 141 mg/L for the river. During the dry season, TN concentrations were all below 20 mg/L, and were not significantly different among sampling stations along the dambo transect. Dry season faecal coliform units (fcu) were significantly different and were 37.2, 30.0 and 5.0 for upland wells, garden wells and river respectively. Wet season faecal coliforms were also significantly different and were 428.5, 258.0 and 479.4 fcu for upland wells, garden wells and river respectively. The other measured physico-chemical parameters also varied with sampling position along the transect. It was concluded that TN and fcu in sampled water varied with season and that wet season concentrations were significantly higher than dry season concentrations. High concentrations of faecal coliforms and total N during the wet season was attributed to increased water movement. Water from upland wells, garden wells and river was not suitable for human consumption according to WHO standards during both the dry and

  12. Phytoplankton, Bacteria and Heterotrophic Nanoflagellate studies using ship and OCM-2 data along a coastal transect in the Arabian Sea

    Digital Repository Service at National Institute of Oceanography (India)

    Matondkar, S.G.P.; Basu, S.; Parab, S.G.; Pednekar, S.; Niyati Hede, N.; Dwivedi, R.M.; Raman, M.; Babu, K.N.; Shukla, A.K.; Shirdhankar, M.M.

    predictable based upon insitu Chl a and Chl a retrieved by the OCM-II and other sensors. Of particular relevance to energy and material cycling in the marine habitat is the trophic relationship between algae and heterotrophic bacteria (Stewart et al. 2004..., Bacteria and Heterotrophic Nanoflagellate studies using ship and OCM-2 data along a coastal transect in the Arabian Sea S. G. Prabhu Matondkar 1 , S. Basu 1 , Sushma G. Parab 1 , Suraksha Pednekar 1 , Niyati Hede 1 , R. M. Dwivedi 2 , Mini Raman 2 , K...

  13. Storage and turnover of organic matter fractions along a Siberian Arctic soil transect

    Science.gov (United States)

    Gentsch, Norman; Mikutta, Robert; Shibistova, Olga; Guggenberger, Georg

    2013-04-01

    Recent observation and climate models demonstrate that arctic ecosystems are already affected by climate warming, as revealed by continuous permafrost degradation and increase of active layer depths. Variations of organic matter (OM) storage in different soil horizons and the OM quality are likely the major drivers of trace gas emissions to the atmosphere. A better understanding of the biogeochemical cycling of OM in permafrost environments is the key to predict future climate changes and the role of terrestrial arctic regions. This study investigates the storage and turnover patterns of OM in functionally different pools, i.e., in particulate plant debris, extractable-water-soluble OM, and mineral-associated OM in permafrost soils along a West-East Siberian transect in the Russian Arctic. We quantified the stocks of total soil organic C (OC) and the respective OM fractions for the first soil meter. Furthermore, we estimated their apparent 14C ages by accelerator mass spectrometry, and determined the mineralization rates and bioavailability of particulate, mineral-bound, and bulk OM in a 90-day incubation experiment. Particulate OM was separated from the mineral-associated OM fraction by density fractionation with sodium polytungstate (density cut-off 1.6 g cm-3) and the OM liberated by this treatment was quantified. Considerable differences in OM storage existed from the West- to the East Siberian Arctic. Cryosols of the Central- and East Siberian sampling sites stored on average 56% more OC than those in West Siberia (25 ± 7 kg m-2versus 11 ± 4 kg m-2 to 1 m soil depth). However, the proportion of the three OM fractions to total OM was similar among the sites. In mineral soil horizons, on average, 17 ± 5% of the total OM was particulate OM, 61 ± 10% was associated with minerals, and 21 ± 3% could be mobilized in dissolved forms during density fractionation. Except for West Siberian soils, ~30% of the OM of the first soil meter was stored in permafrost while

  14. Sustainable Urban Cells and the Energy Transect Modeling: Reconciling the Green and the Urban

    OpenAIRE

    Troglio, Elisabetta; Haas, Tigran

    2013-01-01

    The global city of the 21st century faces major challenges & crises, including social and economic stratification, wasteful consumption of resources, transportation congestion, and environmental degradation with the omnipresence of global climate change. Our cities, communities and neighborhoods are undergoing also rapid transformation and retrofits in terms of energy efficiency and climatic adaptations almost to the point of drastic environmental determinism. The discussion in this paper exp...

  15. Rapid Prototyping Laboratory

    Data.gov (United States)

    Federal Laboratory Consortium — The ARDEC Rapid Prototyping (RP) Laboratory was established in December 1992 to provide low cost RP capabilities to the ARDEC engineering community. The Stratasys,...

  16. Status of amphibians on the Continental Divide:Surveys on a transect from Montana to Colorado, USA

    Science.gov (United States)

    Corn, P.S.; Hossack, B.R.; Muths, E.; Patla, D.A.; Peterson, C.R.; Gallant, A.L.

    2005-01-01

    The Rocky Mountain Region of the United States Geological Survey's Amphibian Research and Monitoring Initiative is conducting monitoring of the status of amphibians on a transect that extends along the Continental Divide from Canada to Colorado and comprises four National Parks. Monitoring uses visual encounter surveys to determine site occupancy, with multiple visits to a subset of sites to estimate detection probabilities for each species. Detection probabilities were generally high (above 0.65) among species. There was a gradient in site occupancy, with most species scarce in the south and relatively common in the north. For example, Bufo boreas is close to extinction in Rocky Mountain National Park, was found at fewer than 5 % of sites in Yellowstone and Grand Teton National Parks in the middle of the transect, but occurs at approximately 10 % of sites in Glacier National Park. The salamander Ambystoma tigrinum was rare in Rocky Mountain and occurred at less than 25 % of sites at Yellowstone and Grand Teton, but A. macrodactylum occurred at more than 50 % of sites in Glacier. There are numerous differences among parks, such as latitude, climate, numbers of visitors, and human population density in the surrounding landscape. The degree to which these factors have influenced the current distribution and abundance of amphibians is unknown but should be a focus of additional research.

  17. Quantitative anatomical and behavioral analyses of regeneration and collateral sprouting following spinal cord transection in the nurse shark (ginglymostoma cirratum).

    Science.gov (United States)

    Gelderd, J B

    1979-01-01

    The spinal cord was transected at the mid-thoracic level in 32 nurse sharks. Four animals per group were sacrificed at intervals of 10, 20, 30, 40, 60 and 90 days postoperative. Two groups of fish underwent a subsequent spinla1 cord retransection at the same site at 90 days and were sacrificed 10 and 20 days later. Three sections of spinal cord were removed from each shark for histological analysis. Behaviorally, timed trials for swimming speed and a strength test for axial musculature contraction caudal to the lesion site were performed at 5 day postoperative intervals. Histological analysis showed little regeneration (9-13 percent) of two descending tracts 90 days following the lesion and no return of rostrally controlled movements caudal to the lesion. However, synaptic readjustment did occur caudal to the lesion. This phenomenon was attributed to local segmental sprouting of adjacent, intact nerve fibers. A close correlation was shown between this synaptic readjustment and the strength of uncontrollable undulatory movements seen caudal to the lesion site following spinal cord transection. The relationship of regeneration and collateral sprouting to quantitative behavioral changes is discussed. PMID:543459

  18. Delayed Presentation of Isolated Complete Pancreatic Transection as a Result of Sport-Related Blunt Trauma to the Abdomen

    Directory of Open Access Journals (Sweden)

    Andrew J. Healey

    2008-01-01

    Full Text Available Introduction: Blunt abdominal trauma is a rare but well-recognized cause of pancreatic transection. A delayed presentation of pancreatic fracture following sport-related blunt trauma with the coexisting diagnostic pitfalls is presented. Case Report: A 17-year-old rugby player was referred to our specialist unit after having been diagnosed with traumatic pancreatic transection, having presented 24 h after a sporting injury. Despite haemodynamic stability, at laparotomy he was found to have a diffuse mesenteric hematoma involving the large and small bowel mesentery, extending down to the sigmoid colon from the splenic flexure, and a large retroperitoneal hematoma arising from the pancreas. The pancreas was completely severed with the superior border of the distal segment remaining attached to the splenic vein that was intact. A distal pancreatectomy with spleen preservation and evacuation of the retroperitoneal hematoma was performed. Discussion/Conclusion: Blunt pancreatic trauma is a serious condition. Diagnosis and treatment may often be delayed, which in turn may drastically increase morbidity and mortality. Diagnostic difficulties apply to both paraclinical and radiological diagnostic methods. A high index of suspicion should be maintained in such cases, with a multi-modality diagnostic approach and prompt surgical intervention as required.

  19. Estimating Synaphobranchus kaupii densities: Contribution of fish behaviour to differences between bait experiments and visual strip transects

    Science.gov (United States)

    Trenkel, Verena M.; Lorance, Pascal

    2011-01-01

    Kaup's arrowtooth eel Synaphobranchus kaupii is a small-bodied fish and an abundant inhabitant of the European continental slope. To estimate its local density video information using the remotely operated vehicle (ROV) Victor 6000 were collected at three locations in the Bay of Biscay slope. Two methods for estimating local densities were tested: strip transect counts and bait experiments. For bait experiments three behaviour types were observed in about equal proportions for individuals arriving near the seafloor: moving up the current towards the ROV, moving across the current and drifting with the current. Visible attraction towards the bait was the highest for individuals swimming against the current (80%) and about equally low for the other two types (around 30%); it did not depend on current speed nor temperature. Three main innovations were introduced for estimating population densities from bait experiments: (i) inclusion of an additional behaviour category—that of passively drifting individuals, (ii) inclusion of reaction behaviour for actively swimming individuals and (iii) a hierarchical Bayesian estimation framework. The results indicated that about half of individuals were foraging actively of which less than one third reacted on encountering the bait plume and the other half were drifting with the current. Taking account of drifting individuals and the reaction probability made density estimates from bait experiments and strip transects more similar.

  20. Diversity and distribution patterns of Pronophilina butterflies (Lepidoptera: Nymphalidae: Satyrinae) along an altitudinal transect in north-western Ecuador.

    Science.gov (United States)

    Pyrcz, Tomasz W; Wojtusiak, Janusz; Garlacz, Rafaa

    2009-01-01

    Samplings of Pronophilina, a species-rich group of neotropical montane butterflies, were carried out along an elevational transect in Ecuador to assess the effect of altitude on their distribution patterns, diversity and community structure. All diversity indices were significantly correlated with altitude. Maximum diversity expressed in species-richness, Shannon index and Fisher alpha was recorded at 2600 m. Two assemblages of species were identified in the lower (below 2100 m) and upper (above 2300 m) sections of the transect by means of correspondence (CA) and cluster analysis. A comparison of Sørensen similarity coefficients showed lower values, thus higher turnover in the intermediate elevational band. Several closely related morphologically and ecologically species were found to have mutually exclusive altitudinal distribution patterns. A comparison with similar studies in Venezuela, Colombia and Peru revealed far reaching congruency of the patterns of altitudinal diversity of Pronophilina in distant areas of the Andes. In particular, the Shannon index reaches its maximum values at 2600-2850 m, which invariably correspond to ca. 400-500 m below the upper limit of cloud forest. Increase of diversity of Pronophilina with altitude is marginally related to higher limited resource availability. The lower pressure of predators and parasites at higher elevation can contribute with higher abundance, but cannot be directly correlated with higher diversity. Higher diversity is related with intrisic characteristics of the group, such as aggregated diversity by overlapping of elevational faunal assemblages and higher speciation ratio towards high elevations, particularly near timberline.

  1. Rapid Tooling via Stereolithography

    OpenAIRE

    Montgomery, Eva

    2006-01-01

    Approximately three years ago, composite stereolithography (SL) resins were introduced to the marketplace, offering performance features beyond what traditional SL resins could offer. In particular, the high heat deflection temperatures and high stiffness of these highly filled resins have opened the door to several new rapid prototyping (RP) applications, including wind tunnel test modelling and, more recently, rapid tooling.

  2. A belt transect setting strategy for mark-recapture experiments to evaluate the 1D diffusion coefficient of beached litter in the cross-shore direction.

    Science.gov (United States)

    Hinata, Hirofumi; Kataoka, Tomoya

    2016-08-15

    We propose a belt transect setting strategy for mark-recapture experiments (MREs) to evaluate the time-independent 1D diffusion coefficient (〈Dp0〉) of marine litter in the cross-shore direction that determines the backwashing flux of the litter, based on two-year MREs for plastic floats (PFs) on Wadahama Beach, Nii-jima Island, Japan. When the alongshore width of the belt transect (Lt) was of the order of, or longer than, the length scale of wave-induced nearshore current circulation (Lc), the PFs were rarely transported alongshore across the selected transects prior to being backwashed offshore. Thus, the transect residence time became longer and showed a much weaker dependence on the transect position, in contrast to when Lt was even shorter than Lc. We therefore obtained the diffusion coefficients close to the value of (〈Dp0〉) when we set Lt to the order of, or longer than, Lc. PMID:27263978

  3. Rapid Characterization of Shorelines using a Georeferenced Video Mapping System

    Energy Technology Data Exchange (ETDEWEB)

    Anderson, Michael G.; Judd, Chaeli; Marcoe, K.

    2012-09-01

    Increased understanding of shoreline conditions is needed, yet current approaches are limited in ability to characterize remote areas or document features at a finer resolution. Documentation using video mapping may provide a rapid and repeatable method for assessing the current state of the environment and determining changes to the shoreline over time. In this study, we compare two studies using boat-based, georeferenced video mapping in coastal Washington and the Columbia River Estuary to map and characterize coastal stressors and functional data. In both areas, mapping multiple features along the shoreline required approximation of the coastline. However, characterization of vertically oriented features such as shoreline armoring and small features such as pilings and large woody debris was possible. In addition, end users noted that geovideo provides a permanent record to allow a user to examine recorded video anywhere along a transect or at discrete points.

  4. Geophysical Modeling of Tectonostratigraphic Terrane Boundaries and Crustal Structure Across a Pacific Ocean-Gulf of Mexico Transect, Southern Mexico

    Science.gov (United States)

    Urrutia-Fucugauchi, J.; Flores-Ruiz, J. H.; Spranger, M.

    2006-12-01

    Geophysical models of terrane boundaries and lithospheric structure beneath southern Mexico derived from gravity and aeromagnetic surveys are presented. The transect crosses southern Mexico from the active Pacific margin to the passive Gulf of Mexico margin, across four distinct terranes (Xolapa, Oaxaca, Juarez and Maya) with Precambrian, Paleozoic and Mesozoic basements and contrasting tectonostratigraphic records. The crust/mantle boundary displays a smooth large amplitude variation along the transect from Puerto Escondido at the Pacific margin to Los Tuxtlas-Alvarado at the Gulf of Mexico, roughly between 28 km and 44 km deep. Crustal thickness variations correspond well with inferred terrane distribution and major surface discontinuities. Suture zones are complex as a result of the kinematics of terrane accretion, contrasting crustal rheological properties, shallow level detachments, post-accretion deformation, thermal conditions and characteristics of relative terrane/plate motions. Pre-suturing characteristics of terranes including crustal structure are difficult to document because of deformation resulting from suturing and any subsequent post-accretion processes. In a simplified way, gravity anomalies from the Pacific margin to the Gulf of Mexico show: large positive 50 mgal anomaly above the continental slope units and the intrusive and metamorphic rocks of the Xolapa complex, then anomalies increasingly negative (with minimum values of -180 mgal over the Acatlan and Oaxaca metamorphics. The Juchatengo mylonitic zone is characterized by a gradient change, while minimum gravity values approximately coincide with the wide mylonitic zone north of Oaxaca city. The Juarez terrane and the region over the Sierra de Juarez is characterized by positive gradient. Finally, the Gulf coastal plain is marked by a positive anomaly in the order of -40 mgal. Geophysical models are combined with the seismic models of the Geolimex profile and used to evaluate the crustal

  5. Spatio-temporal variations and influencing factors of polycyclic aromatic hydrocarbons in atmospheric bulk deposition along a plain-mountain transect in western China

    Science.gov (United States)

    Xing, Xinli; Zhang, Yuan; Yang, Dan; Zhang, Jiaquan; Chen, Wei; Wu, Chenxi; Liu, Hongxia; Qi, Shihua

    2016-08-01

    Ten atmospheric bulk deposition (the sum of wet and dry deposition) samplers for polycyclic aromatic hydrocarbons (PAHs) were deployed at a plain-mountain transect (namely PMT transect, from Daying to Qingping) in Chengdu Plain, West China from June 2007 to June 2008 in four consecutive seasons (about every three months). The bulk deposition fluxes of ∑15-PAHs ranged from 169.19 μg m-2 yr-1 to 978.58 μg m-2 yr-1 with geometric mean of 354.22 μg m-2 yr-1. The most prevalent PAHs were 4-ring (39.65%) and 3-ring (35.56%) PAHs. The flux values were comparable to those in rural areas. Higher fluxes of total PAHs were observed in the middle of PMT transect (SL, YX and JY, which were more urbanized than other sites). The seasonal deposition fluxes in the sampling profile indicated seasonality of the contaminant source was an important factor in controlling deposition fluxes. PAHs bulk deposition was negatively correlated with meteorological parameters (temperature, wind speed, humidity, and precipitation). No significant correlations between soil concentrations and atmospheric deposition were found along this transect. PAHs in soil samples had combined sources of coal, wood and petroleum combustion, while a simple source of coal, wood and grass combustion for bulk deposition. There were significant positive correlation relationship (p Mountain cold trap effect existed in this transect where the altitude was higher than 1000 m. Long-range transport had an impact on the bulk deposition in summer. And this transect was a source to Tibetan only in summer. The forward trajectory analysis showed most air masses did not undergo long-range transport due to the blocking effect of surrounding mountains. Only a few air masses (<10%) arrived at the eastern and northern region of China or farther regions via long-range transport.

  6. SAP Sector Develops Rapidly

    Institute of Scientific and Technical Information of China (English)

    Zheng Chengwang

    2007-01-01

    @@ Stable demand growth internationally Super absorbent polymers (SAP) feature high water absorption, high water retention, rapid water absorption, great expanding power,strong thickening, strong anchoring and excellent elasticity.

  7. Rapid Lead Screening Test

    Science.gov (United States)

    ... Medical Procedures In Vitro Diagnostics Lab Tests Rapid Lead Screening Test Share Tweet Linkedin Pin it More ... reducing the need for a follow-up visit. Lead Risk Links Centers for Disease Control and Prevention ( ...

  8. Morphological and electrophysiological evidence for regeneration of transected spinal cord fibers and restoration of motor functions in adult rats

    Institute of Scientific and Technical Information of China (English)

    2006-01-01

    After 2/3 transection of the right ninth thoracic spinal cord of an adult rat, a chitosan tube seeded with L-poly-lysine was implanted between the rostral and caudal end of the lesioned cord. Twelve months after the operation, regeneration of myelinated and non-myelinated axons and new blood vessels were observed along the wall of the chitosan tube implanted under an electron microscope. Somatosensory evoked potentials (SEP) could be consistently recorded from the left somatosensory cortex following electrical stimulation of the right hind limb, while transcranial magnetic stimulation of the left motor cortex could also evoke motor activity from the right hind limb. The present result suggests that implanted chitosan tube might be useful in regeneration of injured nerve fibers of the spinal cord resulting in a long-term restoration of motor functions.

  9. Crustal deformation styles along the reprocessed deep seismic reflection transect of the Central Iberian Zone (Iberian Peninsula)

    Science.gov (United States)

    Ehsan, Siddique Akhtar; Carbonell, Ramon; Ayarza, Puy; Martí, David; Pérez-Estaún, Andrés; Martínez-Poyatos, David Jesús; Simancas, Jose Fernando; Azor, Antonio; Mansilla, Luis

    2014-05-01

    The multichannel normal incidence (230 km long) deep seismic reflection profile ALCUDIA was acquired in summer 2007. This transect samples an intracontinental Variscan orogenic crust going across, from north to south, the major crustal domain (the Central Iberian Zone) and its suture zone with the Ossa-Morena Zone (the Central Unit) both build up most of the southwestern part of the Iberian Peninsula basement. This high resolution (60-90 fold) profile images about 70 km depth (20 s TWTT) of the continental lithosphere. A new data processing flow provides better structural constraints on the shallow and deep structures resulting in an image that reveals indentation features which strongly suggest horizontal tectonics. The ALCUDIA seismic image shows an upper crust c. 13 km thick decoupled from the comparatively reflective lower crust. The shallow reflectivity of the upper crust can be correlated with surface geological features mapped in the field whereas the deep reflectivity represents inferred imbricate thrust systems and listric extensional faults. The reflectivity of the mid-lower crust is continuous, high amplitude, and horizontal to arcuate though evidences of deformation are present as ductile boudinage structures, thrusting and an upper mantle wedge, suggesting a transpressional flower structure. The image reveals a laminated c. 1.5 km thick, subhorizontal to flat Moho indicating an average crustal thickness of 31-33 km. The Moho shows laterally variable signature, being highly reflective beneath the Central Iberian Zone, but discontinuous and diffuse below the Ossa-Morena Zone. The gravity response suggests relatively high density bodies in the mid-lower crust of the southern half of the transect. The seismic results suggest two major horizontal limits, a horizontal discontinuity at c. 13-15 km (corresponding to the brittle-ductile transition) and the Moho boundary both suggested to act as decoupling surfaces.

  10. S-waves velocity model for the SW-Iberia derived from the IBERSEIS wide-angle seismic reflection transects

    Science.gov (United States)

    Palomeras, I.; Marti, D.; Carbonell, R.; Ayarza, P.; Simancas, F.; Martinez-Poyatos, D.; Azor, A.; Gonzalez-Lodeiro, F.; Perez-Estaun, A.

    2009-04-01

    The IBERSEIS wide-angle seismic reflection transects acquired in 2003 in SW-Iberia Peninsula provided constraints on the P-wave seismic velocity structure across the three tectonic provinces in the area: the South Portuguesse Zone (SPZ), the Ossa-Morena Zone (OMZ) and the Central Iberia Zone (CIZ). These data were acquired by 650 vertical component seismographs (TEXAN seismic recorders) from the IRIS-PASSCAL Instrument center, using explosive sources with charge sizes ranging from 500 to 1000 kg. Both transects A and B are, approximately, 300 km long with a station spacing of 400 m and of 150 m respectively. The relatively small station spacing favored the lateral correlation of the seismic events and provided enough resolution for the identification of shear-wave arrivals. The most prominent S-wave phase recorded by the vertical component sensors corresponds to the SmS which is nearly horizontal for a velocity reduction of 4600 m/s. This phase can even be followed up to near vertical incidence at 18 s(twtt). A few S-wave crustal arrivals can be also identified, although at small offsets they interfere with the previous P-wave arrivals. The Sn phase can be observed at very far offsets providing additional constraints on the nature of the shallow subcrustal mantle. Furthermore, slant stacks of the shot gathers (tau-p sections) reveal the existing of PS energy. PS phases are more difficult to identify in the shot gathers. Finally, a preliminary S-wave velocity model has been derived by iterative forward modeling to provide additional constraints on the nature of the deep crust and upper mantle beneath the Variscan of SW-Iberia.

  11. Tissue effects in vessel sealing and transection from an ultrasonic device with more intelligent control of energy delivery

    Directory of Open Access Journals (Sweden)

    Broughton D

    2013-09-01

    Full Text Available Duan Broughton,1 Alissa L Welling,1 Emily H Monroe,1 Kristen Pirozzi,2 John B Schulte,1 Jeffrey W Clymer1 1Ethicon Endo-Surgery, Inc., Cincinnati, OH, 2University of Central Florida, Orlando, FL, USA Background: Ultrasonic surgical devices have been demonstrated to provide excellent hemostasis, efficient transection, minimal lateral thermal damage, low smoke generation, and no risk of electrical current passage to the patient. These benefits originate from the inherent characteristics of the ultrasonic mechanism, but further improvements were thought possible through optimization of the energy delivery during application. The study reported here compared a new ultrasonic device, Harmonic ACE®+ Shears with Adaptive Tissue Technology, with a commercial predicate device, Harmonic ACE® Shears (both Ethicon Endo-Surgery, Inc., Cincinnati, OH, USA. Methods: Devices were evaluated in an in vivo porcine model intraoperatively and after a 30-day survival period. Both devices were used to seal a variety of vessels 1–5 mm in diameter, and compared for hemostasis, histological thermal damage, and adhesion formation. Sealed vessels were evaluated ex vivo for burst pressure, and visual obstruction caused by plumage from device application was assessed quantitatively. Results: ACE+ produced significantly less thermal damage, had fewer adhesions, offered faster transection, involved less visual obstruction, and had higher burst pressures than the predicate device. All vessel seals, evaluated over the course of a 30-day survival period in pigs, remained intact. Conclusion: The new Adaptive Tissue Technology algorithm assists the surgeon in achieving better control of energy delivery to the tissue, sealing vessels with supra-physiological burst pressures, and low thermal damage. These preclinical results with Harmonic ACE+ may translate into meaningful clinical benefits, providing greater precision along with more efficient and effective cutting and

  12. Lycium barbarum (wolfberry reduces secondary degeneration and oxidative stress, and inhibits JNK pathway in retina after partial optic nerve transection.

    Directory of Open Access Journals (Sweden)

    Hongying Li

    Full Text Available Our group has shown that the polysaccharides extracted from Lycium barbarum (LBP are neuroprotective for retinal ganglion cells (RGCs in different animal models. Protecting RGCs from secondary degeneration is a promising direction for therapy in glaucoma management. The complete optic nerve transection (CONT model can be used to study primary degeneration of RGCs, while the partial optic nerve transection (PONT model can be used to study secondary degeneration of RGCs because primary degeneration of RGCs and secondary degeneration can be separated in location in the same retina in this model; in other situations, these types of degeneration can be difficult to distinguish. In order to examine which kind of degeneration LBP could delay, both CONT and PONT models were used in this study. Rats were fed with LBP or vehicle daily from 7 days before surgery until sacrifice at different time-points and the surviving numbers of RGCs were evaluated. The expression of several proteins related to inflammation, oxidative stress, and the c-jun N-terminal kinase (JNK pathways were detected with Western-blot analysis. LBP did not delay primary degeneration of RGCs after either CONT or PONT, but it did delay secondary degeneration of RGCs after PONT. We found that LBP appeared to exert these protective effects by inhibiting oxidative stress and the JNK/c-jun pathway and by transiently increasing production of insulin-like growth factor-1 (IGF-1. This study suggests that LBP can delay secondary degeneration of RGCs and this effect may be linked to inhibition of oxidative stress and the JNK/c-jun pathway in the retina.

  13. Investigating the influence of PFC transection and nicotine on dynamics of AMPA and NMDA receptors of VTA dopaminergic neurons

    Directory of Open Access Journals (Sweden)

    Chen Ting

    2011-10-01

    Full Text Available Abstract Background All drugs of abuse, including nicotine, activate the mesocorticolimbic system that plays critical roles in nicotine reward and reinforcement development and triggers glutamatergic synaptic plasticity on the dopamine (DA neurons in the ventral tegmental area (VTA. The addictive behavior and firing pattern of the VTA DA neurons are thought to be controlled by the glutamatergic synaptic input from prefrontal cortex (PFC. Interrupted functional input from PFC to VTA was shown to decrease the effects of the drug on the addiction process. Nicotine treatment could enhance the AMPA/NMDA ratio in VTA DA neurons, which is thought as a common addiction mechanism. In this study, we investigate whether or not the lack of glutamate transmission from PFC to VTA could make any change in the effects of nicotine. Methods We used the traditional AMPA/NMDA peak ratio, AMPA/NMDA area ratio, and KL (Kullback-Leibler divergence analysis method for the present study. Results Our results using AMPA/NMDA peak ratio showed insignificant difference between PFC intact and transected and treated with saline. However, using AMPA/NMDA area ratio and KL divergence method, we observed a significant difference when PFC is interrupted with saline treatment. One possible reason for the significant effect that the PFC transection has on the synaptic responses (as indicated by the AMPA/NMDA area ratio and KL divergence may be the loss of glutamatergic inputs. The glutamatergic input is one of the most important factors that contribute to the peak ratio level. Conclusions Our results suggested that even within one hour after a single nicotine injection, the peak ratio of AMPA/NMDA on VTA DA neurons could be enhanced.

  14. Weight-bearing locomotion in the developing opossum, Monodelphis domestica following spinal transection: remodeling of neuronal circuits caudal to lesion.

    Directory of Open Access Journals (Sweden)

    Benjamin J Wheaton

    Full Text Available Complete spinal transection in the mature nervous system is typically followed by minimal axonal repair, extensive motor paralysis and loss of sensory functions caudal to the injury. In contrast, the immature nervous system has greater capacity for repair, a phenomenon sometimes called the infant lesion effect. This study investigates spinal injuries early in development using the marsupial opossum Monodelphis domestica whose young are born very immature, allowing access to developmental stages only accessible in utero in eutherian mammals. Spinal cords of Monodelphis pups were completely transected in the lower thoracic region, T10, on postnatal-day (P7 or P28 and the animals grew to adulthood. In P7-injured animals regrown supraspinal and propriospinal axons through the injury site were demonstrated using retrograde axonal labelling. These animals recovered near-normal coordinated overground locomotion, but with altered gait characteristics including foot placement phase lags. In P28-injured animals no axonal regrowth through the injury site could be demonstrated yet they were able to perform weight-supporting hindlimb stepping overground and on the treadmill. When placed in an environment of reduced sensory feedback (swimming P7-injured animals swam using their hindlimbs, suggesting that the axons that grew across the lesion made functional connections; P28-injured animals swam using their forelimbs only, suggesting that their overground hindlimb movements were reflex-dependent and thus likely to be generated locally in the lumbar spinal cord. Modifications to propriospinal circuitry in P7- and P28-injured opossums were demonstrated by changes in the number of fluorescently labelled neurons detected in the lumbar cord following tracer studies and changes in the balance of excitatory, inhibitory and neuromodulatory neurotransmitter receptors' gene expression shown by qRT-PCR. These results are discussed in the context of studies indicating

  15. Abundance of Two Pelagibacter ubique Bacteriophage Genotypes along a Latitudinal Transect in the North and South Atlantic Oceans

    Science.gov (United States)

    Eggleston, Erin M.; Hewson, Ian

    2016-01-01

    This study characterizes viral and bacterial dynamics along a latitudinal transect in the Atlantic Ocean from approximately 10 N–40 S. Overall viral abundance decreased with depth, on average there were 1.64 ± 0.71 × 107 virus like particles (VLPs) in surface waters, decreasing to an average of 6.50 ± 2.26 × 105 VLPs in Antarctic Bottom Water. This decrease was highly correlated to bacterial abundance. There are six major water masses in the Southern Tropical Atlantic Ocean, and inclusion of water mass, temperature and salinity variables explained a majority of the variation in total viral abundance. Recent discovery of phages infecting bacteria of the SAR11 clade of Alphaproteobacteria (i.e., pelagiphages) leads to intriguing questions about the roles they play in shaping epipelagic communities. Viral-size fraction DNA from epipelagic water was used to quantify the abundance of two pelagiphages, using pelagiphage-specific quantitative PCR primers and probes along the transect. We found that HTVC010P, a member of a podoviridae sub-family, was most abundant in surface waters. Copy numbers ranged from an average of 1.03 ± 2.38 × 105 copies ml−1 in surface waters, to 5.79 ± 2.86 × 103 in the deep chlorophyll maximum. HTVC008M, a T4-like myovirus, was present in the deep chlorophyll maximum (5.42 ± 2.8 × 103 copies ml−1 on average), although it was not as highly abundant as HTVC010P in surface waters (6.05 ± 3.01 × 103 copies ml−1 on average). Interestingly, HTVC008M was only present at a few of the most southern stations, suggesting latitudinal biogeography of SAR11 phages. PMID:27733846

  16. Ocean-scale patterns in community respiration rates along continuous transects across the Pacific Ocean.

    Science.gov (United States)

    Wilson, Jesse M; Severson, Rodney; Beman, J Michael

    2014-01-01

    Community respiration (CR) of organic material to carbon dioxide plays a fundamental role in ecosystems and ocean biogeochemical cycles, as it dictates the amount of production available to higher trophic levels and for export to the deep ocean. Yet how CR varies across large oceanographic gradients is not well-known: CR is measured infrequently and cannot be easily sensed from space. We used continuous oxygen measurements collected by autonomous gliders to quantify surface CR rates across the Pacific Ocean. CR rates were calculated from changes in apparent oxygen utilization and six different estimates of oxygen flux based on wind speed. CR showed substantial spatial variation: rates were lowest in ocean gyres (mean of 6.93 mmol m(-3) d(-1)±8.0 mmol m(-3) d(-1) standard deviation in the North Pacific Subtropical Gyre) and were more rapid and more variable near the equator (8.69 mmol m(-3) d(-1)±7.32 mmol m(-3) d(-1) between 10°N and 10°S) and near shore (e.g., 5.62 mmol m(-3) d(-1)±45.6 mmol m(-3) d(-1) between the coast of California and 124°W, and 17.0 mmol m(-3) d(-1)±13.9 mmol m(-3) d(-1) between 156°E and the Australian coast). We examined how CR varied with coincident measurements of temperature, turbidity, and chlorophyll concentrations (a proxy for phytoplankton biomass), and found that CR was weakly related to different explanatory variables across the Pacific, but more strongly related to particular variables in different biogeographical areas. Our results indicate that CR is not a simple linear function of chlorophyll or temperature, and that at the scale of the Pacific, the coupling between primary production, ocean warming, and CR is complex and variable. We suggest that this stems from substantial spatial variation in CR captured by high-resolution autonomous measurements.

  17. Rapid small lot manufacturing

    Energy Technology Data Exchange (ETDEWEB)

    Harrigan, R.W.

    1998-05-09

    The direct connection of information, captured in forms such as CAD databases, to the factory floor is enabling a revolution in manufacturing. Rapid response to very dynamic market conditions is becoming the norm rather than the exception. In order to provide economical rapid fabrication of small numbers of variable products, one must design with manufacturing constraints in mind. In addition, flexible manufacturing systems must be programmed automatically to reduce the time for product change over in the factory and eliminate human errors. Sensor based machine control is needed to adapt idealized, model based machine programs to uncontrolled variables such as the condition of raw materials and fabrication tolerances.

  18. Rapid microbiology - raising awareness.

    Science.gov (United States)

    Bailie, Jonathan

    2016-01-01

    A 'high-level overview' of some of the emerging rapid microbiology technologies designed to help healthcare engineering and infection control teams working in hospitals and other healthcare facilities more rapidly identify potentially hazardous levels of waterborne microorganisms in their water systems, enabling them to take prompt remedial action, and a look at the some of the 'pros and cons' of such testing techniques, was given by Nalco technical director, Howard Barnes, the vice-chair of the Legionella Control Association (LCA), at a recent LCA open day. HEJ editor, Jonathan Bailie, reports.

  19. FBSAD RECRUIT Reef Fish Belt Transect Survey at Hawaii Island (Big Island), Main Hawaiian Islands, 2008; and Midway Atoll, Northwestern Hawaiian Islands (NWHI), 2008 (NODC Accession 0067519)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Shore-based belt transects were conducted at 1 to ~ 5 m depths at a total four (4) sites: at (1-2) 2 longshore sites on the leeward coast (South Kohala district) of...

  20. Nadir videos taken during low-altitude transects of the Arctic Network of national park units and Selawik National Wildlife Refuge, Alaska, July 2013

    Data.gov (United States)

    U.S. Geological Survey, Department of the Interior — Nadir videos shot from a GoPro Hero3 Black Edition (focal length 2.77 mm) of the arctic landscape during low altitude transects at the rate of 60 frames per second....

  1. Testing the Validity of the Random Quadrat and Line-Transect Methods for Estimating Field Populations--or 'String and Coverslip' Ecology

    Science.gov (United States)

    Hitchon, Arnold

    1975-01-01

    Uses a printed sheet of paper to represent a plant population in a given area. Quadrat sampling is simulated by dropping a square coverslip on the sheet and recording the symbols under the coverslip. Line-transect method is simulated by having a student randomly adjust the sheet under a string. (GS)

  2. The Morphofunctional Effect of the Transplantation of Bone Marrow Stromal Cells and Predegenerated Peripheral Nerve in Chronic Paraplegic Rat Model via Spinal Cord Transection

    Science.gov (United States)

    Buzoianu-Anguiano, Vinnitsa; Orozco-Suárez, Sandra; García-Vences, Elisa; Caballero-Chacón, Sara; Guizar-Sahagún, Gabriel; Chavez-Sanchez, Luis; Grijalva, Israel

    2015-01-01

    Functional recovery following spinal cord injury (SCI) is limited by poor axonal and cellular regeneration as well as the failure to replace damaged myelin. Employed separately, both the transplantation of the predegenerated peripheral nerve (PPN) and the transplantation of bone marrow stromal cells (BMSCs) have been shown to promote the regrowth and remyelination of the damaged central axons in SCI models of hemisection, transection, and contusion injury. With the aim to test the effects of the combined transplantation of PPN and BMSC on regrowth, remyelination, and locomotor function in an adult rat model of spinal cord (SC) transection, 39 Fischer 344 rats underwent SC transection at T9 level. Four weeks later they were randomly assigned to traumatic spinal cord injury (TSCI) without treatment, TSCI + Fibrin Glue (FG), TSCI + FG + PPN, and TSCI + FG + PPN + BMSCs. Eight weeks after, transplantation was carried out on immunofluorescence and electron microscope studies. The results showed greater axonal regrowth and remyelination in experimental groups TSCI + FG + PPN and TSCI + FG + PPN + BMSCs analyzed with GAP-43, neuritin, and myelin basic protein. It is concluded that the combined treatment of PPN and BMSCs is a favorable strategy for axonal regrowth and remyelination in a chronic SC transection model. PMID:26634157

  3. The Morphofunctional Effect of the Transplantation of Bone Marrow Stromal Cells and Predegenerated Peripheral Nerve in Chronic Paraplegic Rat Model via Spinal Cord Transection.

    Science.gov (United States)

    Buzoianu-Anguiano, Vinnitsa; Orozco-Suárez, Sandra; García-Vences, Elisa; Caballero-Chacón, Sara; Guizar-Sahagún, Gabriel; Chavez-Sanchez, Luis; Grijalva, Israel

    2015-01-01

    Functional recovery following spinal cord injury (SCI) is limited by poor axonal and cellular regeneration as well as the failure to replace damaged myelin. Employed separately, both the transplantation of the predegenerated peripheral nerve (PPN) and the transplantation of bone marrow stromal cells (BMSCs) have been shown to promote the regrowth and remyelination of the damaged central axons in SCI models of hemisection, transection, and contusion injury. With the aim to test the effects of the combined transplantation of PPN and BMSC on regrowth, remyelination, and locomotor function in an adult rat model of spinal cord (SC) transection, 39 Fischer 344 rats underwent SC transection at T9 level. Four weeks later they were randomly assigned to traumatic spinal cord injury (TSCI) without treatment, TSCI + Fibrin Glue (FG), TSCI + FG + PPN, and TSCI + FG + PPN + BMSCs. Eight weeks after, transplantation was carried out on immunofluorescence and electron microscope studies. The results showed greater axonal regrowth and remyelination in experimental groups TSCI + FG + PPN and TSCI + FG + PPN + BMSCs analyzed with GAP-43, neuritin, and myelin basic protein. It is concluded that the combined treatment of PPN and BMSCs is a favorable strategy for axonal regrowth and remyelination in a chronic SC transection model. PMID:26634157

  4. The Morphofunctional Effect of the Transplantation of Bone Marrow Stromal Cells and Predegenerated Peripheral Nerve in Chronic Paraplegic Rat Model via Spinal Cord Transection

    Directory of Open Access Journals (Sweden)

    Vinnitsa Buzoianu-Anguiano

    2015-01-01

    Full Text Available Functional recovery following spinal cord injury (SCI is limited by poor axonal and cellular regeneration as well as the failure to replace damaged myelin. Employed separately, both the transplantation of the predegenerated peripheral nerve (PPN and the transplantation of bone marrow stromal cells (BMSCs have been shown to promote the regrowth and remyelination of the damaged central axons in SCI models of hemisection, transection, and contusion injury. With the aim to test the effects of the combined transplantation of PPN and BMSC on regrowth, remyelination, and locomotor function in an adult rat model of spinal cord (SC transection, 39 Fischer 344 rats underwent SC transection at T9 level. Four weeks later they were randomly assigned to traumatic spinal cord injury (TSCI without treatment, TSCI + Fibrin Glue (FG, TSCI + FG + PPN, and TSCI + FG + PPN + BMSCs. Eight weeks after, transplantation was carried out on immunofluorescence and electron microscope studies. The results showed greater axonal regrowth and remyelination in experimental groups TSCI + FG + PPN and TSCI + FG + PPN + BMSCs analyzed with GAP-43, neuritin, and myelin basic protein. It is concluded that the combined treatment of PPN and BMSCs is a favorable strategy for axonal regrowth and remyelination in a chronic SC transection model.

  5. FBSAD PREDATOR Reef Fish Belt Transect Survey at Hawaii Island (Big Island), Main Hawaiian Islands, 2008; and Midway Atoll, Northwestern Hawaiian Islands (NWHI), 2008 (NODC Accession 0067519)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Shore-based belt transects were conducted at 1 to ~5 m depths at a total four (4) sites: at (1-2) 2 longshore sites on the leeward coast (South Kohala district) of...

  6. Ocean-scale patterns in community respiration rates along continuous transects across the Pacific Ocean.

    Directory of Open Access Journals (Sweden)

    Jesse M Wilson

    Full Text Available Community respiration (CR of organic material to carbon dioxide plays a fundamental role in ecosystems and ocean biogeochemical cycles, as it dictates the amount of production available to higher trophic levels and for export to the deep ocean. Yet how CR varies across large oceanographic gradients is not well-known: CR is measured infrequently and cannot be easily sensed from space. We used continuous oxygen measurements collected by autonomous gliders to quantify surface CR rates across the Pacific Ocean. CR rates were calculated from changes in apparent oxygen utilization and six different estimates of oxygen flux based on wind speed. CR showed substantial spatial variation: rates were lowest in ocean gyres (mean of 6.93 mmol m(-3 d(-1±8.0 mmol m(-3 d(-1 standard deviation in the North Pacific Subtropical Gyre and were more rapid and more variable near the equator (8.69 mmol m(-3 d(-1±7.32 mmol m(-3 d(-1 between 10°N and 10°S and near shore (e.g., 5.62 mmol m(-3 d(-1±45.6 mmol m(-3 d(-1 between the coast of California and 124°W, and 17.0 mmol m(-3 d(-1±13.9 mmol m(-3 d(-1 between 156°E and the Australian coast. We examined how CR varied with coincident measurements of temperature, turbidity, and chlorophyll concentrations (a proxy for phytoplankton biomass, and found that CR was weakly related to different explanatory variables across the Pacific, but more strongly related to particular variables in different biogeographical areas. Our results indicate that CR is not a simple linear function of chlorophyll or temperature, and that at the scale of the Pacific, the coupling between primary production, ocean warming, and CR is complex and variable. We suggest that this stems from substantial spatial variation in CR captured by high-resolution autonomous measurements.

  7. Spatio-temporal variations and influencing factors of polycyclic aromatic hydrocarbons in atmospheric bulk deposition along a plain-mountain transect in western China

    Science.gov (United States)

    Xing, Xinli; Zhang, Yuan; Yang, Dan; Zhang, Jiaquan; Chen, Wei; Wu, Chenxi; Liu, Hongxia; Qi, Shihua

    2016-08-01

    Ten atmospheric bulk deposition (the sum of wet and dry deposition) samplers for polycyclic aromatic hydrocarbons (PAHs) were deployed at a plain-mountain transect (namely PMT transect, from Daying to Qingping) in Chengdu Plain, West China from June 2007 to June 2008 in four consecutive seasons (about every three months). The bulk deposition fluxes of ∑15-PAHs ranged from 169.19 μg m-2 yr-1 to 978.58 μg m-2 yr-1 with geometric mean of 354.22 μg m-2 yr-1. The most prevalent PAHs were 4-ring (39.65%) and 3-ring (35.56%) PAHs. The flux values were comparable to those in rural areas. Higher fluxes of total PAHs were observed in the middle of PMT transect (SL, YX and JY, which were more urbanized than other sites). The seasonal deposition fluxes in the sampling profile indicated seasonality of the contaminant source was an important factor in controlling deposition fluxes. PAHs bulk deposition was negatively correlated with meteorological parameters (temperature, wind speed, humidity, and precipitation). No significant correlations between soil concentrations and atmospheric deposition were found along this transect. PAHs in soil samples had combined sources of coal, wood and petroleum combustion, while a simple source of coal, wood and grass combustion for bulk deposition. There were significant positive correlation relationship (p atmospheric bulk deposition and local PAHs emission, with biomass burning as the major contribution to the total emission of PAHs. This transect acts as an important PAHs source rather than being a sink according to the ratio of deposition/emission. Mountain cold trap effect existed in this transect where the altitude was higher than 1000 m. Long-range transport had an impact on the bulk deposition in summer. And this transect was a source to Tibetan only in summer. The forward trajectory analysis showed most air masses did not undergo long-range transport due to the blocking effect of surrounding mountains. Only a few air masses (<10

  8. Fisheries Biology and Stock Assessment Division (FBSAD) Recruit and Predator Reef Fish Belt Transect and Habitat Quadrat Surveys at Hawaii Island (Big Island), Main Hawaiian Islands, 2008; and Midway Atoll, Northwestern Hawaiian Islands (NWHI), 2008 (NODC Accession 0067519)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Shore-based belt transects were conducted and habitat quadrats were surveyed using transects at 1 to ~ 5 m depths at a total four (4) sites: at (1-2) 2 longshore...

  9. Navigate the Digital Rapids

    Science.gov (United States)

    Lindsay, Julie; Davis, Vicki

    2010-01-01

    How can teachers teach digital citizenship when the digital landscape is changing so rapidly? How can teachers teach proper online social interactions when the students are outside their classroom and thus outside their control? Will encouraging students to engage in global collaborative environments land teachers in hot water? These are the…

  10. Temporal dynamics of patterns and structure of electrical conductivity and water storage along three transects irrigated with water at different salinity levels

    Science.gov (United States)

    Saeed, Ali; Ajeel, Ali; Dragonetti, Giovanna; Comegna, Alessandro; Lamaddalena, Nicola; Coppola, Antonio

    2015-04-01

    This study evaluated the role of local scale soil heterogeneities on water and salinity distribution in the soil. The study used a large spatial and temporal dataset of Electromagnetic Induction (EMI), Time Domain Reflectometry (TDR) and Diviner 2000 sensor readings collected at regularly spaced monitoring sites and in several time campaigns along three transects irrigated with water at different salinity levels. The experiment was carried out in a 558m2 field at the Mediterranean Agronomic Institute of Bari, south-eastern coast of Italy. The experimental field consisted of three adjacent transects, 30 m long and 4.2 m wide, with a distance between transects of 3.0 m. The three transects were irrigated with irrigation water at three different salinity levels (1dSm-1, 3dSm-1, 6dSm-1). The irrigation volumes were calculated according to the time-dynamics of water storage measured by the Diviner 2000 capacitance sensor. For each transect, thirteen access tubes, 2 m apart, were installed along the middle line at 60 cm depth to monitor water content in the soil before and after irrigation. A Fourier's analysis was applied to explore the different patterns and structures of variability of the original TDR data series. The analysis was also applied to filter the original data series, in order to extract the predominant, high-variance signal after removing the noise of both the EMI and TDR data series. It is our opinion that identifying the predominant patterns of variability should reveal specific features of the space-time variability of soil water content and salinity to be explicitly used for upscaling water flow and salt transport processes from local to field scale.

  11. Quantitative mineral proxies of fluid chemistry and geothermal gradients in the Kumano Transect, Nankai Trough, Japan

    Science.gov (United States)

    Sample, James; Weeks, Sarah; Fisher, Andrew; Defliese, Will; Tripati, Aradhna; IOPD Expedition 348 Science Party

    2016-04-01

    interval at 2105 mbsf, fall mostly below the gradient, even though the trend in oxygen isotope values from several cements and veins suggests formation along a geotherm. The carbonates could have formed at shallower depths, and the apparent trend along a geotherm is fortuitious. Another possibility is that the geothermal gradient decreases more rapidly at depth than has been modeled, and that the carbonates are a record of recent temperatures. If the latter is correct, then there is a clear temperature spike of ~20°C from the 2105 fault that records flow of exotic fluid from depth. Additional carbonate clumped analyses are underway on samples from beneath the Kumano Basin. In accretionary margins it is common to observe carbonate formation in the upper 10s to 100s of meters, above the sulfate-methane transition zone, but our results show at greater depths processes continue to favor dissolution and reprecipitation of carbonate, especially in faults, thus contributing to sediment lithification before and during deformation. The relationship of veins with faults supports faults as prevalent conduits. In some cases it is clear that carbonates can provide quantitative estimates of thermal gradients where other temperature data are not available. Such information is essential for understanding other processes that can change physical properties.

  12. Physical vs. Chemical Weathering Controls of Soils' Capacity to Store Carbon: Hillslope Transects under Different Climatic Conditions

    Science.gov (United States)

    Yoo, K.; Wackett, A.; Amundson, R.; Heimsath, A. M.

    2015-12-01

    Soil C storage is balanced by photosynthetic production and microbial decomposition of organic matter (OM). Recently, this view has been expanded to account for the effects of physical erosion of OM in determining soil C storage. In parallel, the focus on OM quality as a primary determinant of C turnover has shifted to OM-mineral interactions. These recent advances necessitates our ability to discern how physical erosion, which controls the production, breakdown, and removal of colluvial soils, and chemical weathering, which generates secondary phyllosilicate and iron oxides, independently and collaboratively affect soils' capacity to store C. Here we present soil organic C contents and storages as a function of soil properties that are controlled by physical vs. chemical weathering processes. The study site includes two hillslopes under different climates in SW Australia. The wetter site has continuous canopy of eucalyptus, while the drier site is covered by grasses with scattered eucalyptus overstorey. The two hillslope transects share similar granodiorite parent materials and denudation rates. Bioturbation-driven soil creep appears equally effective at both sites. In eroding areas, chemical weathering has created greater mineral surface area in the soils of wetter site, while physical soil production and erosion resulted in forming the eroding soils of similar thicknesses at both sites. In the drier site, however, vegetation density varies significantly with topography-dependent soil moisture, which appears to have resulted in a soil toposequence where impacts of localized overland-flow erosion is evident through soil mineral surface area, texture, and C contents. These soil properties, in contrast, are largely homogeneous across the wetter hillslope transect presumably because of the lack of localized overland-flow erosion. As a result, at the depositional areas, the drier site exhibits greater or similar soil C storages, which sharply contrasts with the

  13. The effects of the mineral phase on C stabilization mechanisms and the microbial community along an eroding slope transect

    Science.gov (United States)

    Doetterl, S.; Opfergelt, S.; Cornelis, J.; Boeckx, P. F.; van oost, K.; Six, J.

    2013-12-01

    An increasing number of studies show the importance of including soil redistribution processes in understanding carbon (C) dynamics in eroding landscapes. The quality and quantity of soil organic carbon in sloping cropland differs with topographic position. These differences are commonly more visible in the subsoil, while the size and composition of topsoil C pools are similar along the hillslope. The type (plant- or microbial-derived) and quality (level of degradation) of C found in a specific soil fraction depends on the interplay between the temporal dynamic of the specific mechanism and it's strength to protect C from decomposition. Here, we present an analysis that aims to clarify the bio/geo-chemical and mineralogical components involved in stabilizing C at various depths and slope positions and how they affect the microbial community and the degradation of C. For this we analyzed soil samples from different soil depths along a slope transect applying (i) a sequential extraction of the reactive soil phase using pyrophosphate, oxalate and dithionite-citrate-bicarbonate, (ii) a semi-quantitative and qualitative analysis of the clay mineralogy, (iii) an analysis of the microbial community using amino sugars and (iv) an analysis of the level of degradation of C in different soil fractions focusing on the soil Lignin signature. The results show that the pattern of minerals and their relative importance in stabilizing C varies greatly along the transect. In the investigated soils, pyrophosphate extractable Manganese, and not Iron or Aluminum as often observed, is strongly correlated to C in the bulk soil and in the non-aggregated silt and clay fractions. This suggests a certain role of Manganese for C stabilization where physical protection is absent. In contrast, pyrophosphate extractable Iron and Aluminum components are largely abundant in water-stable soil aggregates but not correlated to C, suggesting importance of these extracts to stabilize aggregates and

  14. Quantitative T2 mapping evaluation for articular cartilage lesions in a rabbit model of anterior cruciate ligament transection osteoarthritis

    Institute of Scientific and Technical Information of China (English)

    WEI Zheng-mao; DU Xiang-ke; HUO Tian-long; LI Xu-bin; QUAN Guang-nan; LI Tian-ran; CHENG Jin; ZHANG Wei-tao

    2012-01-01

    Background Quantitative T2 mapping has been a widely used method for the evaluation of pathological cartilage properties,and the histological assessment system of osteoarthritis in the rabbit has been published recently.The aim of the study was to investigate the effectiveness of quantitative T2 mapping evaluation for articular cartilage lesions of a rabbit model of anterior cruciate ligament transection (ACLT) osteoarthritis.Methods Twenty New Zealand White (NZW) rabbits were divided into ACLT surgical group and sham operated group equally.The anterior cruciate ligaments of the rabbits in ACLT group were transected,while the joints were closed intactly in sham operated group.Magnetic resonance (MR) examinations were performed on 3.0T MR unit at week 0,week 6,and week 12.T2 values were computed on GE ADW4.3 workstation.All rabbits were killed at week 13,and left knees were stained with Haematoxylin and Eosin.Semiquantitative histological grading was obtained according to the osteoarthritis cartilage histopathology assessment system.Computerized image analysis was performed to quantitate the immunostained collagen type Ⅱ.Results The average MR T2 value of whole left knee cartilage in ACLT surgical group ((29.05±12.01) ms) was significantly higher than that in sham operated group ((24.52±7.97) ms) (P=0.024) at week 6.The average T2 value increased to (32.18±12.79) ms in ACLT group at week 12,but remained near the baseline level ((27.66±8.08) ms) in the sham operated group (P=0.03).The cartilage lesion level of left knee in ACLT group was significantly increased at week 6 (P=0.005) and week 12 (P <0.001).T2 values had positive correlation with histological grading scores,but inverse correlation with optical densities (OD) of type Ⅱ collagen.Conclusion This study demonstrated the reliability and practicability of quantitative T2 mapping for the cartilage injury of rabbit ACLT osteoarthritis model.

  15. Cenozoic variations in the South Atlantic carbonate saturation profile: Insights from the Walvis depth-transect (ODP Leg 208)

    Science.gov (United States)

    Schellenberg, S. A.; Nielsen, J. L.

    2004-12-01

    Ocean Drilling Program Leg 208 Science Party (D. Kroon, J. C. Zachos, P. Blum, J. Bowles, P. Gaillot, T. Hasegawa, E. C. Hawthorne, D. A. Hodell, D. C. Kelly, J. Jung, S. M. Keller, Y. Lee, D. C. Leuschner, Z. Liu, K. C. Lohmann, L. Lourens, S. Monechi, M. Nicolo, I. Raffi, C. Riesselman, U. Röhl, D. Schmidt, A. Sluijs, D. Thomas, E. Thomas, H. Vallius) Carbonate saturation profiles are complex and dynamic products of processes operating on temporospatial scales from the "short-term local" (e.g. carbonate export production) to the "long-term global" (e.g. carbonate-silicate weathering, shelf:basin carbonate partitioning). Established, if admittedly crude, proxies for reconstructing carbonate saturation from sediments include wt% carbonate, where values of 0-20% are typically attributed to deposition below the carbonate compensation depth (CCD), and planktonic foraminifer fragmentation, where enhanced fragmentation is typically attributed to deposition below the lysocline. Ocean Drilling Program Leg 208 successfully drilled a six-site Walvis Ridge depth-transect spanning modern water depths from 2,717 to 4,755 m. Exceptional core recovery, well-constrained biomagnetostratigraphy, and standard crustal subsidence corrections provide a working age-depth framework for contouring ship-board wt% carbonate determinations and identifying the following first-order features of the regional CCD: (1) >3.5 km position from 60-48 Ma punctuated by a major transient shoaling to 4.25 km from 37 to 28 Ma; (4) marked high amplitude fluctuations from 28 to 20 Ma followed by deepening to >4.75 km; (5) transient shoaling to ˜4 km around 15 Ma followed by deepening to >4.75 km by ˜12 Ma. These first-order features are broadly congruent with classic Atlantic CCD reconstructions by van Andel (1975) and Berger and Roth (1975). A wealth of higher frequency variation in carbonate saturation is clearly preserved within the Leg 208 depth-transect. Ongoing shore-based analyses aim to transform

  16. Age-dependent changes in the proteome following complete spinal cord transection in a postnatal South American opossum (Monodelphis domestica.

    Directory of Open Access Journals (Sweden)

    Natassya M Noor

    Full Text Available Recovery from severe spinal injury in adults is limited, compared to immature animals who demonstrate some capacity for repair. Using laboratory opossums (Monodelphis domestica, the aim was to compare proteomic responses to injury at two ages: one when there is axonal growth across the lesion and substantial behavioural recovery and one when no axonal growth occurs. Anaesthetized pups at postnatal day (P 7 or P28 were subjected to complete transection of the spinal cord at thoracic level T10. Cords were collected 1 or 7 days after injury and from age-matched controls. Proteins were separated based on isoelectric point and subunit molecular weight; those whose expression levels changed following injury were identified by densitometry and analysed by mass spectrometry. Fifty-six unique proteins were identified as differentially regulated in response to spinal transection at both ages combined. More than 50% were cytoplasmic and 70% belonged to families of proteins with characteristic binding properties. Proteins were assigned to groups by biological function including regulation (40%, metabolism (26%, inflammation (19% and structure (15%. More changes were detected at one than seven days after injury at both ages. Seven identified proteins: 14-3-3 epsilon, 14-3-3 gamma, cofilin, alpha enolase, heart fatty acid binding protein (FABP3, brain fatty acid binding protein (FABP7 and ubiquitin demonstrated age-related differential expression and were analysed by qRT-PCR. Changes in mRNA levels for FABP3 at P7+1day and ubiquitin at P28+1day were statistically significant. Immunocytochemical staining showed differences in ubiquitin localization in younger compared to older cords and an increase in oligodendrocyte and neuroglia immunostaining following injury at P28. Western blot analysis supported proteomic results for ubiquitin and 14-3-3 proteins. Data obtained at the two ages demonstrated changes in response to injury, compared to controls, that were

  17. Erraticity of rapidity gaps

    International Nuclear Information System (INIS)

    The use of rapidity gaps is proposed as a measure of the spatial pattern of an event. When the event multiplicity is low, the gaps between neighboring particles carry far more information about an event than multiplicity spikes, which may occur very rarely. Two moments of the gap distribution are suggested for characterizing an event. The fluctuations of those moments from event to event are then quantified by an entropy-like measure, which serves to describe erraticity. We use ECOMB to simulate the exclusive rapidity distribution of each event, from which the erraticity measures are calculated. The dependences of those measures on the order q of the moments provide single-parameter characterizations of erraticity. (c) 2000 The American Physical Society

  18. Rapid Detection of Pathogens

    Energy Technology Data Exchange (ETDEWEB)

    David Perlin

    2005-08-14

    Pathogen identification is a crucial first defense against bioterrorism. A major emphasis of our national biodefense strategy is to establish fast, accurate and sensitive assays for diagnosis of infectious diseases agents. Such assays will ensure early and appropriate treatment of infected patients. Rapid diagnostics can also support infection control measures, which monitor and limit the spread of infectious diseases agents. Many select agents are highly transmissible in the early stages of disease, and it is critical to identify infected patients and limit the risk to the remainder of the population and to stem potential panic in the general population. Nucleic acid-based molecular approaches for identification overcome many of the deficiencies associated with conventional culture methods by exploiting both large- and small-scale genomic differences between organisms. PCR-based amplification of highly conserved ribosomal RNA (rRNA) genes, intergenic sequences, and specific toxin genes is currently the most reliable approach for bacterial, fungal and many viral pathogenic agents. When combined with fluorescence-based oligonucleotide detection systems, this approach provides real-time, quantitative, high fidelity analysis capable of single nucleotide allelic discrimination (4). These probe systems offer rapid turn around time (<2 h) and are suitable for high throughput, automated multiplex operations that are critical for clinical diagnostic laboratories. In this pilot program, we have used molecular beacon technology invented at the Public health Research Institute to develop a new generation of molecular probes to rapidly detect important agents of infectious diseases. We have also developed protocols to rapidly extract nucleic acids from a variety of clinical specimen including and blood and tissue to for detection in the molecular assays. This work represented a cooperative research development program between the Kramer-Tyagi/Perlin labs on probe development

  19. New Rapid Spore Assay

    Science.gov (United States)

    Kminek, Gerhard; Conley, Catharine

    2012-07-01

    The presentation will detail approved Planetary Protection specifications for the Rapid Spore Assay for spacecraft components and subsystems. Outlined will be the research and studies on which the specifications were based. The research, funded by ESA and NASA/JPL, was conducted over a period of two years and was followed by limited cleanroom studies to assess the feasibility of this assay during spacecraft assembly.

  20. Tiber Personal Rapid Transit

    Directory of Open Access Journals (Sweden)

    Diego Carlo D'agostino

    2011-02-01

    Full Text Available The project “Tiber Personal Rapid Transit” have been presented by the author at the Rome City Vision Competition1 2010, an ideas competition, which challenges architects, engineers, designers, students and creatives individuals to develop visionary urban proposals with the intention of stimulating and supporting the contemporary city, in this case Rome. The Tiber PRT proposal tries to answer the competition questions with the definition of a provocative idea: a Personal Rapid transit System on the Tiber river banks. The project is located in the central section of the Tiber river and aims at the renewal of the river banks with the insertion of a Personal Rapid Transit infrastructure. The project area include the riverbank of Tiber from Rome Transtevere RFI station to Piazza del Popolo, an area where main touristic and leisure attractions are located. The intervention area is actually no used by the city users and residents and constitute itself a strong barrier in the heart of the historic city.

  1. Rapid frequency scan EPR.

    Science.gov (United States)

    Tseitlin, Mark; Rinard, George A; Quine, Richard W; Eaton, Sandra S; Eaton, Gareth R

    2011-08-01

    In rapid frequency scan EPR with triangular scans, sufficient time must be allowed to insure that the magnetization in the x, y plane decays to baseline at the end of the scan, which typically is about 5T(2) after the spins are excited. To permit relaxation of signals excited toward the extremes of the scan the total scan time required may be much longer than 5T(2). However, with periodic, saw-tooth excitation, the slow-scan EPR spectrum can be recovered by Fourier deconvolution of data recorded with a total scan period of 5T(2), even if some spins are excited later in the scan. This scan time is similar to polyphase excitation methods. The peak power required for either polyphase excitation or rapid frequency scans is substantially smaller than for pulsed EPR. The use of an arbitrary waveform generator (AWG) and cross loop resonator facilitated implementation of the rapid frequency scan experiments reported here. The use of constant continuous low B(1), periodic excitation waveform, and constant external magnetic field is similar to polyphase excitation, but could be implemented without the AWG that is required for polyphase excitation. PMID:21664848

  2. Effective Elastic Thickness of Southeast Part of Arctic Ocean-Eurasia Continent-Pacific Ocean Geoscience Transect

    Institute of Scientific and Technical Information of China (English)

    2002-01-01

    The effective lithospheric elastic thickness of the continent is an important parameter for examination of the large-scale structure and analyses of the mechanism of isostatic compensation within the plate, and a parameter standing for the strength of the lithosphere. The Te values along QuanzhouHeishui, the southeast section of the Arctic Ocean-Eurasian Continent-Pacific Ocean geoscience transect,are estimated by using the coherence technique developed by Forsyth. Studies of the feature of the coherence between gravity and topography suggest that at short wavelengths (6.6-100 km) for each data box that is used to estimate Te, the plate is strong enough to support topographic loads and gravity and topography are uncorrelated. At long wavelengths where the plate is deflected by surface and subsurface loads are compensated by the flexure model. Sichuan land-stone with Iow heat-flow values has high Te values whereas in Ninghua, Datian, land-stone with high heat-flow values has low To values, which reflects a correlation, iow heat-flow values corresponding to high Te values and high heat-flow values corresponding to low Te values. Te values can be divided into two sections: northwest high section and southeast iow section. There is roughly a positive correlation between crustal thickness and effective elastic thickness of the lithosphere.

  3. Linking the soil moisture distribution pattern to dynamic processes along slope transects in the Loess Plateau, China.

    Science.gov (United States)

    Wang, Shuai; Fu, Bojie; Gao, Guangyao; Zhou, Ji; Jiao, Lei; Liu, Jianbo

    2015-12-01

    Soil moisture pulses are a prerequisite for other land surface pulses at various spatiotemporal scales in arid and semi-arid areas. The temporal dynamics and profile variability of soil moisture in relation to land cover combinations were studied along five slopes transect on the Loess Plateau during the rainy season of 2011. Within the 3 months of the growing season coupled with the rainy season, all of the soil moisture was replenished in the area, proving that a type stability exists between different land cover soil moisture levels. Land cover combinations disturbed the trend determined by topography and increased soil moisture variability in space and time. The stability of soil moisture resulting from the dynamic processes could produce stable patterns on the slopes. The relationships between the mean soil moisture and vertical standard deviation (SD) and coefficient of variation (CV) were more complex, largely due to the fact that different land cover types had distinctive vertical patterns of soil moisture. The spatial SD of each layer had a positive correlation and the spatial CV exhibited a negative correlation with the increase in mean soil moisture. The soil moisture stability implies that sampling comparisons in this area can be conducted at different times to accurately compare different land use types. PMID:26620951

  4. The essential oil qualitative and quantitative composition in the needles of Pinus sylvestris L. growing along industrial transects

    Energy Technology Data Exchange (ETDEWEB)

    Kupcinskiene, Eugenija [Department of Biology, Vytautas Magnus University, Vileikos 8, LT-44404 Kaunas (Lithuania)], E-mail: e.kupcinskiene@gmail.com; Stikliene, Aida [Department of Ecology, Lithuanian University of Agriculture, Studentu 11, LT-53361 Kaunas, Akademija (Lithuania); Judzentiene, Asta [Institute of Chemistry, A. Gostauto 9, LT-01108 Vilnius (Lithuania)

    2008-10-15

    The aim of this study was to evaluate composition of the essential oils in the needles of Pinus sylvestris growing in the areas affected by a cement factory (CF), and an oil refinery (OR). Volatile components of the needles were analyzed by GC and GC/MS. The most heavily polluted CF stand had significantly higher concentration of {gamma}-Terpinene, Caryophyllene oxide in the current-year needles, while higher concentration of {delta}-3-Carene, {alpha}-Terpinene, {gamma}-Terpinene and Terpinolene was documented for 1-year-old needles. The most heavily polluted OR stand had a significantly higher concentration of Sabinene + {beta}-Pinene, 1-epi-Cubenol in the current-year needles and a significantly higher concentration of Camphene, Sabinene + {beta}-Pinene, Myrcene, {alpha}-Cadinene, 1-epi-Cubenol in the 1-year-old needles than the least polluted site. Along transects an increase in the amount of some diterpenes and a decrease in the components of the shorter chain essential oils was observed. These effects could be at least partially attributed to SO{sub 2}. - Quantities of components of essential oil in the needles of Scots pine growing around two factories are described in relation to pollution.

  5. Linking the soil moisture distribution pattern to dynamic processes along slope transects in the Loess Plateau, China.

    Science.gov (United States)

    Wang, Shuai; Fu, Bojie; Gao, Guangyao; Zhou, Ji; Jiao, Lei; Liu, Jianbo

    2015-12-01

    Soil moisture pulses are a prerequisite for other land surface pulses at various spatiotemporal scales in arid and semi-arid areas. The temporal dynamics and profile variability of soil moisture in relation to land cover combinations were studied along five slopes transect on the Loess Plateau during the rainy season of 2011. Within the 3 months of the growing season coupled with the rainy season, all of the soil moisture was replenished in the area, proving that a type stability exists between different land cover soil moisture levels. Land cover combinations disturbed the trend determined by topography and increased soil moisture variability in space and time. The stability of soil moisture resulting from the dynamic processes could produce stable patterns on the slopes. The relationships between the mean soil moisture and vertical standard deviation (SD) and coefficient of variation (CV) were more complex, largely due to the fact that different land cover types had distinctive vertical patterns of soil moisture. The spatial SD of each layer had a positive correlation and the spatial CV exhibited a negative correlation with the increase in mean soil moisture. The soil moisture stability implies that sampling comparisons in this area can be conducted at different times to accurately compare different land use types.

  6. Burial Diagenesis of Magnetic Minerals: New Insights from the Grès d’Annot Transect (SE France

    Directory of Open Access Journals (Sweden)

    Myriam Kars

    2014-07-01

    Full Text Available The diagenetic evolution of the magnetic minerals during burial in sedimentary basins has been recently proposed. In this study, we provide new data from the Grès d’Annot basin, SE France. We analyze fine-grained clastic rocks that suffered a burial temperature from ~60 to >250 °C, i.e., covering oil and gas windows. Low temperature magnetic measurements (10–300 K, coupled with vitrinite reflectance data, aim at defining the magnetic mineral evolution through the burial history. Magnetite is documented throughout the entire studied transect. Goethite, probably occurring as nanoparticles, is found for a burial temperature <80 °C. Micron-sized pyrrhotite is highlighted for a burial temperature >200 °C below the Alpine nappes and the Penninic Front. A model of the evolution of the magnetic assemblage from 60 to >250 °C is proposed for clastic rocks, containing iron sulfides (pyrite and organic matter. This work provides the grounds for a better understanding of the magnetic properties of petroleum plays.

  7. Hydrodynamism, crude oil distribution and geochemistry of the stratigraphic column in a transect of the Eastern Venezuelan Basin

    Energy Technology Data Exchange (ETDEWEB)

    Gallango, O.; Escandon, M.; Alberdi, M. (Intevep, S.A. Caracas (Venezuela)); Parnaud, F.; Pascual, J.C. (Beicip, Rueil Malmaison (France))

    1992-01-01

    The hydrocarbon accumulation history in a transect of the Eastern Venezuelan Basin is closely related to the generation and migration process as a consequence of the stratigraphic, structural and tectonic evolution of the basin during the Cretaceous and Cenozoic times. Thermal maturity assessment based on kinetic parameters using the appropriate software indicates that the generation of oil from the marine facies of the Querecual and San Antonio source rocks in the northern part of the basin took place during early Paleocene to early Miocene times prior to the start of thrusting, while the second and third phase occurred during middle Miocene to recent times in zones beneath the principal thrust in the Maturing area. The third phase of oil generation is associated with the latter thrusts which are out of sequence. Taking into consideration the development of the oil and gas kitchen through time, the hydrodynamism, the characteristics and distribution of the hydrocarbons, the migration of oil and gas have been outlined. The major differences observed among the oils, are due to maturity and post-accumulation processes such as oil mixing and biodegradation. A wide range of thermal maturity was observed in the oils, which is mainly due to the thermal stress experienced by the source rock. Association of light crudes with meteoric waters have been observed, as well as heavy to extra-heavy crudes with connate waters at greater depth. These unusual associations of crudes and formation waters are related to the hydrodynamical regime in the basin.

  8. Rock magnetic properties of a loess-paleosol couple along an N-S transect in the Chinese Loess Plateau

    Institute of Scientific and Technical Information of China (English)

    2001-01-01

    We have conducted detailed rock magnetic experiments on samples from loess unit 8(L8) and paleosol unit 8 (S8) in Jingbian, Yichuan and Duanjiapo loess sections along an N-S transect in the Chinese Loess Plateau. Major rock magnetic results are as follows: ( i ) An increase of high field susceptibility (χh) in the same level of loess or paleosol from north to south is observed, suggesting an enhancement of pedogenesis. (ii) The Iow field susceptibility (χL) in loess unit L8 is almost the same in three sections. In contrast, the χL of paleosol unit S8 in Yichuan is highest, and the χL of Duanjiapo is lower than that in Yichuan section, suggesting that there is not correlation between the χL and the degree of pedogenesis in loess-paleosols. (iii) With the increasing of χL, both the contents of the superparamagnetic (SP) and the ferrimagnetic grains in Ioess-paleosol increase, however, the enhancement of magnetic susceptibility is probably dependent more on the increase of the ferrimagnetic concentration than on a change in the grain size. (iv) The content of the maghemite in loess unit 8 increases from the northern to the southern part of the Chinese Loess Plateau, and is positively correlated with the pedogenesis of the loess.

  9. Shifts in methanogen community structure and function across a coastal marsh transect: effects of exotic Spartina alterniflora invasion

    Science.gov (United States)

    Yuan, Junji; Ding, Weixin; Liu, Deyan; Kang, Hojeong; Xiang, Jian; Lin, Yongxin

    2016-01-01

    Invasion of Spartina alterniflora in coastal areas of China increased methane (CH4) emissions. To elucidate the underlying mechanisms, we measured CH4 production potential, methanogen community structure and biogeochemical factors along a coastal wetland transect comprised of five habitat regions: open water, bare tidal flat, invasive S. alterniflora marsh and native Suaeda salsa and Phragmites australis marshes. CH4 production potential in S. alterniflora marsh was 10 times higher than that in other regions, and it was significantly correlated with soil organic carbon, dissolved organic carbon and trimethylamine concentrations, but was not correlated with acetate or formate concentrations. Although the diversity of methanogens was lowest in S. alterniflora marsh, invasion increased methanogen abundance by 3.48-fold, compared with native S. salsa and P. australis marshes due to increase of facultative Methanosarcinaceae rather than acetotrophic and hydrogenotrophic methanogens. Ordination analyses suggested that trimethylamine was the primary factor regulating shift in methanogen community structure. Addition of trimethylamine increased CH4 production rates by 1255-fold but only by 5.61- and 11.4-fold for acetate and H2/CO2, respectively. S. alterniflora invasion elevated concentration of non-competitive trimethylamine, and shifted methanogen community from acetotrophic to facultative methanogens, which together facilitated increased CH4 production potential.

  10. Sulfasalazine attenuates ACL transection and medial menisectomy-induced cartilage destruction by inhibition of cystine/glutamate antiporter.

    Science.gov (United States)

    Tsai, Wei-Yuan; Tsai, Ru-Yin; Liu, Chih-Chung; Wu, Jia-Lin; Wong, Chih-Shung

    2016-04-01

    We had previously demonstrated that excitatory amino acid glutamate plays a role in the progression and severity of knee osteoarthritis (OA), and early hyaluronic acid injection attenuates the OA progression by attenuation of knee joint glutamate level, which was also related to the cystine/glutamate antiporter system X (system XC-) expression. System XC- uptakes cystine into chondrocytes for glutathione (GSH) synthesis, but the role of system XC- in OA is rarely addressed. Sulfasalazine (SSZ) is a system XC- inhibitor; SSZ was applied intra-articularly to study the function of system XC- in the development of OA in rats subjected to anterior cruciate ligament transection and medial meniscectomy (ACLT + MMx). Moerover, the system XC- activator N-acetylcysteine (NAC) was also applied to verify the role of system XC-. The intra-articular injection of SSZ significantly attenuated knee swelling and cartilage destruction in the knees of ACLT + MMx rats and this effect was blocked by NAC. The results showed that inhibition of system XC- function can attenuate ACLT + MMx-induced cartilage destruction. In the present study, system XC- inhibitor SSZ was shown to reduce glutamate content in synovial fluid and GSH in chondrocytes. It was also showed SSZ could attenuate ACLT + MMx-induced cartilage destruction, and treatment of NAC reversed the protective effect of SSZ.

  11. Right-Rapid-Rough

    Science.gov (United States)

    Lawrence, Craig

    2003-01-01

    IDEO (pronounced 'eye-dee-oh') is an international design, engineering, and innovation firm that has developed thousands of products and services for clients across a wide range of industries. Its process and culture attracted the attention of academics, businesses, and journalists around the world, and are the subject of a bestselling book, The Art of Innovation by Tom Kelley. One of the keys to IDEO's success is its use of prototyping as a tool for rapid innovation. This story covers some of IDEO's projects, and gives reasons for why they were successful.

  12. Rapid Frequency Scan EPR

    OpenAIRE

    Tseitlin, Mark; Rinard, George A.; Quine, Richard W.; Eaton, Sandra S.; Eaton, Gareth R.

    2011-01-01

    In rapid frequency scan EPR with triangular scans, sufficient time must be allowed to insure that the magnetization in the x,y plane decays to baseline at the end of the scan, which typically is about 5 T2 after the spins are excited. To permit relaxation of signals excited toward the extremes of the scan the total scan time required may be much longer than 5 T2. However, with periodic, saw-tooth excitation, the slow-scan EPR spectrum can be recovered by Fourier deconvolution of data recorded...

  13. Rapidly progressive tabetic neurosyphilis

    Institute of Scientific and Technical Information of China (English)

    赖伟红; 薛华忠; 韩国柱

    2003-01-01

    Since the sexually transmitted diseases were recognized as a public health problem in China during the early 1980's, the incidence of syphilis has gradually increased. Though there have been case reports of clinical variants of neurosyphilis, including syphilitic cerebrospinal meningitis or meningomyelitis and meningovascular syphilis, occurring in different regions of China,1-3 tabes dorsalis or tabetic neurosyphilis has not yet been reported in China. Here, we report a young man with rapidly progressive tabetic neurosyphilis admitted to our hospital in October 1999.

  14. Implementation of a Functional Observation Battery for the Assessment of Postoperative Well-being in Rats Subjected to Fimbria-Fornix Transection

    DEFF Research Database (Denmark)

    Marschner, Linda; Wogensen, Elise; Mogensen, Jesper;

    2016-01-01

    -fornix transected animals (FF), sham-operated animals (Sham), and two non-operated control groups with and without administration of buprenorphine (+BUP and -BUP, respectively) were observed twice daily for seven days after surgery. Buprenorphine (0.4 mg/kg) mixed in a nut paste for voluntary ingestion was supplied...... twice daily for 84 h to all groups except the -BUP control group starting on the day of surgery. Body weight was slightly decreased postoperatively in both surgical groups (FF and Sham) compared to control groups. The +BUP control group lost weight starting at day four after discontinuation......, the FOB implemented in the present study appears to be a sensitive and accurate protocol for assessing animal well-being in the experimental setup applied. It is apparent that the FF transection is an invasive procedure that causes mildly adverse postoperative effects on the rats' well-being. We therefore...

  15. The Rapid Transient Surveyor

    CERN Document Server

    Baranec, Christoph; Wright, Shelley A; Tonry, John; Tully, R Brent; Szapudi, István; Takamiya, Marianne; Hunter, Lisa; Riddle, Reed; Chen, Shaojie; Chun, Mark

    2016-01-01

    The Rapid Transient Surveyor (RTS) is a proposed rapid-response, high-cadence adaptive optics (AO) facility for the UH 2.2-m telescope on Maunakea. RTS will uniquely address the need for high-acuity and sensitive near-infrared spectral follow-up observations of tens of thousands of objects in mere months by combining an excellent observing site, unmatched robotic observational efficiency, and an AO system that significantly increases both sensitivity and spatial resolving power. We will initially use RTS to obtain the infrared spectra of ~4,000 Type Ia supernovae identified by the Asteroid Terrestrial-Impact Last Alert System over a two year period that will be crucial to precisely measuring distances and mapping the distribution of dark matter in the z < 0.1 universe. RTS will comprise an upgraded version of the Robo-AO laser AO system and will respond quickly to target-of-opportunity events, minimizing the time between discovery and characterization. RTS will acquire simultaneous-multicolor images with a...

  16. Tetraether membrane lipid distributions in water-column particulate matter and sediments : a study of 47 European lakes along a north–south transect

    OpenAIRE

    Blaga, C. I.; Reichart, G.-J.; O. Heiri; Sinninghe Damsté, J.S.

    2008-01-01

    We studied the distribution of glycerol dialkyl glycerol tetraethers (GDGTs) in water-column particulate matter and the top 5 cm of sediment from 47 lakes along a transect from southern Italy to the northern part of Scandinavia. Our objective was to investigate the biological sources and potential palaeoenvironmental applications of GDGTs in lacustrine sediments. Both archaea-derived isoprenoid and bacteria-derived branched GDGTs, produced by yet unknown soil bacteria, were identified in all ...

  17. The Morphofunctional Effect of the Transplantation of Bone Marrow Stromal Cells and Predegenerated Peripheral Nerve in Chronic Paraplegic Rat Model via Spinal Cord Transection

    OpenAIRE

    Vinnitsa Buzoianu-Anguiano; Sandra Orozco-Suárez; Elisa García-Vences; Sara Caballero-Chacón; Gabriel Guizar-Sahagún; Luis Chavez-Sanchez; Israel Grijalva

    2015-01-01

    Functional recovery following spinal cord injury (SCI) is limited by poor axonal and cellular regeneration as well as the failure to replace damaged myelin. Employed separately, both the transplantation of the predegenerated peripheral nerve (PPN) and the transplantation of bone marrow stromal cells (BMSCs) have been shown to promote the regrowth and remyelination of the damaged central axons in SCI models of hemisection, transection, and contusion injury. With the aim to test the effects of ...

  18. Electro-acupuncture promotes survival, differentiation of the bone marrow mesenchymal stem cells as well as functional recovery in the spinal cord-transected rats

    OpenAIRE

    Li Yan; Zhang Yu-Jiao; Li Wen-Jie; Zhang Yan-Qing; Ruan Jing-Wen; Yan Qing; Ding Ying; Dong Hongxin; Zeng Yuan-Shan

    2009-01-01

    Abstract Background Bone marrow mesenchymal stem cells (MSCs) are one of the potential tools for treatment of the spinal cord injury; however, the survival and differentiation of MSCs in an injured spinal cord still need to be improved. In the present study, we investigated whether Governor Vessel electro-acupuncture (EA) could efficiently promote bone marrow mesenchymal stem cells (MSCs) survival and differentiation, axonal regeneration and finally, functional recovery in the transected spin...

  19. H-REFLEX UP-CONDITIONING ENCOURAGES RECOVERY OF EMG ACTIVITY AND H-REFLEXES AFTER SCIATIC NERVE TRANSECTION AND REPAIR IN RATS

    OpenAIRE

    Chen, Yi; Wang, Yu; Chen, Lu; Sun, Chenyuo; English, Arthur W.; Wolpaw, Jonathan R.; Chen, Xiang Yang

    2010-01-01

    Operant conditioning of the spinal stretch reflex or its electrical analog, the H-reflex, produces spinal cord plasticity and can thereby affect motoneuron responses to primary afferent input. To explore whether this conditioning can affect the functional outcome after peripheral nerve injury, we assessed the effect of up-conditioning soleus (SOL) H-reflex on SOL and tibialis anterior (TA) function after sciatic nerve transection and repair. Sprague-Dawley rats were implanted with EMG electro...

  20. The effects of post-pasture woody plant colonization on soil and aboveground litter carbon and nitrogen along a bioclimatic transect

    OpenAIRE

    La Mantia T; Gristina L; Rivaldo E; Pasta S; Novara A; Rühl J.

    2013-01-01

    We investigated the effects of woody plant colonization of abandoned pastures on soil and litter organic carbon (C) stocks and nitrogen (N) content along a bioclimatic transect in a semi-arid environment (Sicily, Italy). Soil samples were taken in three successional stages (grazed pasture, shrubland, forest) within each of three bioclimates (supramediterranean - “supra”, mesomediterranean - “meso”, thermomediterranean - “thermo”). Organic C and N in litter and soil (0-10 cm and 10-30 cm depth...

  1. The carbon isotope record in soils along a forest-cerrado ecosystem transect : implications for vegetation changes in the Rondonia state, Southwestern Brazilian Amazon region

    OpenAIRE

    Pessenda, L.C.R.; Gomes, B.M.; Aravena, R.; Ribeiro, A.S.; Boulet, René; Gouveia, S.E.M.

    1998-01-01

    This paper presents carbon isotope data on soil organic matter (SOM) collected along an ecosystem transect that includes a wooded savanah (cerrado), a tropical semideciduous forest (cerradao), a forest transition type and a tropical forest. The study area is located in the Rondonia state, southwestern Brazilian Amazon region. 14C data of total soil organic matter and charcoal indicate that the organic matter in these soils is a least Holocene in age. The forest and forest transition sites are...

  2. Satellite and ground-based observations of patterns and seasonality of sea-ice, summer warmth, snow, and NDVI along the North America and Eurasia Arctic transects

    Science.gov (United States)

    Walker, D. A.; Epstein, H. E.; Raynolds, M. K.; Bhatt, U. S.; Bieniek, P. A.

    2011-12-01

    We analyzed vegetation, climate, and spectral data from zonal sites along two >1500 km long transects that span all five Arctic bioclimate subzones in North America and Eurasia to help interpret the long-term changes in satellite-derived trends of pattern and seasonality of vegetation greenness. Despite large differences in environment and vegetation along the two transects, there is nearly an identical logarithmic relationship between biomass and the summer maximum normalized difference vegetation index derived from AVHRR sensors (MaxNDVI) along the two transects. Summer open water in the Northern Alaska/Beaufort Sea region has increased by 39%, the summer warmth index (SWI) of the tundra increased by 14%, MaxNDVI by 28% and time-integrated NDVI (TI-NDVI) by 21%. The increased open water in the Beaufort is associated with a warming of the land and a large positive increase in the NDVI. In the eastern Kara Sea/Yamal Peninsula region, summer-fall open water has increased by 115%, the SWI decreased by -3%, MaxNDVI increased by only 6%, and TI-NDVI by 2%. The greatly reduced sea ice has affected the summer total warmth and NDVI of the Eurasia transect minimally possibly due to increased winter snow and delayed snowmelt in much of northwest Russian Arctic. In northern Alaska, there is distinctive trend of earlier snow melt at most stations; whereas the northern Yamal has seen an increase in the snow water equivalent and delayed melt on much of the Yagorsky, Yamal, Gydan, and Taimyr peninsulas. This appears to be associated with the reduction in the total summer warmth and relatively small increase in NDVI.

  3. Using scaling factors for evaluating spatial and temporal variability of soil hydraulic properties within one elevation transect

    Science.gov (United States)

    Nikodem, Antonín; Kodešová, Radka; Jakšík, Ondřej; Fér, Miroslav; Klement, Aleš

    2016-04-01

    This study was carried out in Southern Moravia, in the Czech Republic. The original soil unit in the wider area is a Haplic Chernozem developed on loess. The intensive agricultural exploitation in combination with terrain morphology has resulted in a highly diversified soil spatial pattern. Nowadays the original soil unit is preserved only on top of relatively flat parts, and is gradually transformed by water erosion up to Regosols on the steepest slopes, while colluvial soils are formed in terrain depressions and at toe slopes due to sedimentation of previously eroded material. Soils within this area has been intensively investigated during the last several years (e.g. Jakšík et al., 2015; Vašát et al., 2014, 2015a,b). Soil sampling (disturbed and undisturbed 100-cm3 soil samples) was performed at 5 points of one elevation transect in November 2010 (after wheat sowing) and August 2011 (after wheat harvest). Disturbed soil samples were used to determine basic soil properties (grain size distribution and organic carbon content etc.). Undisturbed soil samples were used to determine the soil water retention curves and the hydraulic conductivity functions using the multiple outflow tests in Tempe cells and a numerical inversion with HYDRUS 1-D. Scaling factors (alpha-h for pressure head, alpha-theta for soil water contents and alpha-k for hydraulic conductivities) were used here to express soil hydraulic properties variability. Evaluated scaling factors reflected position within the elevation transect as well as time of soil sampling. In general large values of alpha-h, lower values of alpha-k and similar values of alpha-theta were obtained in 2010 in comparison to values obtained in 2011, which indicates development of soil structure during the vegetation season. Jakšík, O., Kodešová, R., Kubiš, A., Stehlíková, I., Drábek, O., Kapička, A. (2015): Soil aggregate stability within morphologically diverse areas. Catena, 127, 287-299. Vašát, R., Kode

  4. Lithospheric velocity model across the Southern Central Iberian Zone (Variscan Iberian Massif): The ALCUDIA wide-angle seismic reflection transect

    Science.gov (United States)

    Ehsan, Siddique Akhtar; Carbonell, Ramon; Ayarza, Puy; Martí, David; Martínez Poyatos, David; Simancas, José Fernando; Azor, Antonio; Ayala, Concepción; Torné, Montserrat; Pérez-Estaún, Andrés.

    2015-03-01

    A P wave seismic velocity model has been obtained for the Central Iberian Zone, the largest continental fragment of the Iberian Variscan Belt. The spatially dense, high-resolution, wide-angle seismic reflection experiment, ALCUDIA-WA, was acquired in 2012 across central Iberia, aiming to constrain the lithospheric structure and resolve the physical properties of the crust and upper mantle. The seismic transect, ~310 km long, crossed the Central Iberian Zone from its suture with the Ossa-Morena Zone to the southern limit of the Central System mountain range. The energy generated by five shots was recorded by ~900 seismic stations. High-amplitude phases were identified in every shot gather for the upper crust (Pg and PiP) and Moho (PmP and Pn). In the upper crust, the P wave velocities increase beneath the Cenozoic Tajo Basin. The base of the upper crust varies from ~13 km to ~20 km between the southernmost Central Iberian Zone and the Tajo Basin. Lower crustal velocities are more homogeneous. From SW-NE, the traveltime of PmP arrivals varies from ~10.5 s to ~11.8 s, indicating lateral variations in the P wave velocity and the crustal thickness, reflecting an increase toward the north related with alpine tectonics and the isostatic response of the crust to the orogenic load. The results suggest that the high velocities of the upper crust near the Central System might correspond to igneous rocks and/or high-grade metamorphic rocks. The contrasting lithologies and the increase in the Moho depth to the north evidence differences in the Variscan evolution.

  5. Dissolved low-molecular weight thiol concentrations from the U.S. GEOTRACES North Atlantic Ocean zonal transect

    Science.gov (United States)

    Swarr, Gretchen J.; Kading, Tristan; Lamborg, Carl H.; Hammerschmidt, Chad R.; Bowman, Katlin L.

    2016-10-01

    Low-molecular weight thiols, including cysteine and glutathione, are biomolecules involved in a variety of metabolic pathways and act as important antioxidant and metal buffering agents. In this last capacity, they represent a potential mechanism for modulating the bioavailability and biogeochemistry of many trace elements in the ocean, particularly for chalcophilic elements (e.g., Cu, Zn, Cd, Ag and Hg). For this reason, and in the context of the international GEOTRACES program that seeks to understand the biogeochemistry of trace elements in the ocean, we measured the concentration of individual dissolved low-molecular weight thiols during the U.S. GEOTRACES North Atlantic Zonal Transect (USGNAZT). Only two thiols were identified, cysteine and glutathione, in contrast to results from the northeast subarctic Pacific Ocean, where the dipeptides glycine-cysteine and arginine-cysteine were also present and γ-glutamylcysteine was dominant. Concentrations of cysteine and glutathione in the North Atlantic Ocean were lower than in the Pacific and ranged from below detection (~0.01 nM) to 0.61 nM of cysteine and up to 1.0 nM of glutathione, with cysteine generally more abundant than glutathione. Vertical profiles of cysteine and glutathione were broadly consistent with their biological production, being more abundant in surface water and usually below detection at depths greater than about 200 m. Subsurface concentration maxima, often co-incident with the deep chlorophyll maximum, were frequently observed but not universal. We conclude that cysteine and glutathione do not make up significant portions of complexation capacity for Cu and Zn in the upper open ocean but could be important for Cd, Hg, and potentially other chalcophiles. Extremely low concentrations of cysteine and glutathione in deep water suggest that higher molecular-weight thiols are a more important ligand class for chalcophiles in that portion of the ocean.

  6. Spatiotemporal variations in phytoplankton biomass and community structure in a meridional transect of the East/Japan Sea

    Science.gov (United States)

    Kwak, J. H.; Kang, C. K.; Kang, H.

    2015-12-01

    To better understand the variations in phytoplankton biomass and community composition associated with water-column structure and environmental conditions in the East/Japan Sea (EJS), three cruses were carried out along a meridional transect in May 2007 (spring), July 2009 (summer), and October 2012 (fall). The subpolar front (SPF) was formed between warm and cold water mass (37-40 °N). Chlorophyll a concentration and phytoplankton community composition were studied using HPLC pigment and CHEMTAX analysis and the results showed no significant differences between warm and cold water masses. These results reject our initial hypothesis that different water masses between southern and northern parts of the EJS may lead to different phytoplankton community structure. During the study periods, isotherm layers (≤ 12 °C) fluctuated over 50 m depth between warm and cold water masses on the basis of the SPF. In contrast, the nitracline (i.e. 2.5 μM nitrate isopleths) depth was recorded within the limited depths ranged 20-40 m, 30-50 m, and 40-60 m in spring, summer, and fall, respectively. The chlorophyll a concentrations at the subsurface chlorophyll maxima (SCM) in spring and summer (356 ± 233 and 270 ± 182 ng L-1, respectively) were significantly higher than those in fall (117 ± 89 ng L-1). The relative contributions of phytoplankton groups to total chlorophyll a concentration reflected phytoplankton community composition in the SCM layer with showing a dominance of diatoms (58 ± 19, 48 ± 11, and 30 ± 20 % in spring, summer, and fall, respectively). High contribution of diatoms to total biomass may enhance the efficiency of biological pump in the EJS. In addition, canonical correspondence analysis revealed a clear distribution of phytoplankton groups associated with temperature and nutrient concentration which mean prevalence of vertical variation. Finally, our findings suggested that phytoplankton biomass and groups are regulated by surface mixed layer depth

  7. Trunk robot rehabilitation training with active stepping reorganizes and enriches trunk motor cortex representations in spinal transected rats.

    Science.gov (United States)

    Oza, Chintan S; Giszter, Simon F

    2015-05-01

    Trunk motor control is crucial for postural stability and propulsion after low thoracic spinal cord injury (SCI) in animals and humans. Robotic rehabilitation aimed at trunk shows promise in SCI animal models and patients. However, little is known about the effect of SCI and robot rehabilitation of trunk on cortical motor representations. We previously showed reorganization of trunk motor cortex after adult SCI. Non-stepping training also exacerbated some SCI-driven plastic changes. Here we examine effects of robot rehabilitation that promotes recovery of hindlimb weight support functions on trunk motor cortex representations. Adult rats spinal transected as neonates (NTX rats) at the T9/10 level significantly improve function with our robot rehabilitation paradigm, whereas treadmill-only trained do not. We used intracortical microstimulation to map motor cortex in two NTX groups: (1) treadmill trained (control group); and (2) robot-assisted treadmill trained (improved function group). We found significant robot rehabilitation-driven changes in motor cortex: (1) caudal trunk motor areas expanded; (2) trunk coactivation at cortex sites increased; (3) richness of trunk cortex motor representations, as examined by cumulative entropy and mutual information for different trunk representations, increased; (4) trunk motor representations in the cortex moved toward more normal topography; and (5) trunk and forelimb motor representations that SCI-driven plasticity and compensations had caused to overlap were segregated. We conclude that effective robot rehabilitation training induces significant reorganization of trunk motor cortex and partially reverses some plastic changes that may be adaptive in non-stepping paraplegia after SCI.

  8. Response of heterotrophic and autotrophic microbial plankton to inorganic and organic inputs along a latitudinal transect in the Atlantic Ocean

    Directory of Open Access Journals (Sweden)

    S. Martínez-García

    2010-01-01

    Full Text Available Atmospheric nutrient deposition into the open ocean increased over the past decades as a result of human activity and water-soluble organic nitrogen accounts for up to 30% of the total nitrogen inputs. The effects of inorganic and/or organic nutrient inputs on phytoplankton and heterotrophic bacteria have never been concurrently assessed in open ocean oligotrophic communities over a wide spatial gradient. We studied the effects of potentially limiting inorganic (nitrate, ammonium, phosphate, silica and organic nutrient (glucose, aminoacids inputs on microbial plankton biomass, community structure and metabolism in five microcosm experiments conducted along a latitudinal transect in the Atlantic Ocean (from 26° N to 29° S.

    Primary production rates increased up to 1.8-fold. Bacterial respiration and microbial community respiration increased up to 14.3 and 12.7-fold, respectively. Bacterial production and bacterial growth efficiency increased up to 58.8-fold and 2.5-fold, respectively. The largest increases were measured after mixed inorganic-organic nutrients additions. Changes in microbial plankton biomass were small as compared with those in metabolic rates. A north to south increase in the response of heterotrophic bacteria was observed, which could be related to a latitudinal gradient in phosphorus availability. Our results suggest that organic matter inputs associated with atmospheric deposition into the Atlantic Ocean will result in a predominantly heterotrophic versus autotrophic response and in increases in bacterial growth efficiency, particularly in the Southern Hemisphere. Subtle differences in the initial environmental and biological conditions are likely to result in differential microbial responses to inorganic and organic matter inputs.

  9. Crustal implications of bedrock geology along the Trans-Alaska Crustal Transect (TACT) in the Brooks Range, northern Alaska

    Science.gov (United States)

    Moore, T.E.; Wallace, W.K.; Mull, C.G.; Adams, K.E.; Plafker, G.; Nokleberg, W.J.

    1997-01-01

    Geologic mapping of the Trans-Alaska Crustal Transect (TACT) project along the Dalton Highway in northern Alaska indicates that the Endicott Mountains allochthon and the Hammond terrane compose a combined allochthon that was thrust northward at least 90 km in the Early Cretaceous. The basal thrust of the combined allochthon climbs up section in the hanging wall from a ductile shear zone, in the south through lower Paleozoic rocks of the Hammond terrane and into Upper Devonian rocks of the Endicott Mountains allochthon at the Mount Doonerak antiform, culminating in Early Cretaceous shale in the northern foothills of the Brooks Range. Footwall rocks north of the Mount Doonerak antiform are everywhere parautochthonous Permian and Triassic shale of the North Slope terrane rather than Jurassic and Lower Cretaceous strata of the Colville Basin as shown in most other tectonic models of the central Brooks Range. Stratigraphic and structural relations suggest that this thrust was the basal detachment for Early Cretaceous deformation. Younger structures, such as the Tertiary Mount Doonerak antiform, deform the Early Cretaceous structures and are cored by thrusts that root at a depth of about 10 to 30 km along a deeper detachment than the Early Cretaceous detachment. The Brooks Range, therefore, exposes (1) an Early Cretaceous thin-skinned deformational belt developed during arc-continent collision and (2) a mainly Tertiary thick-skinned orogen that is probably the northward continuation of the Rocky Mountains erogenic belt. A down-to-the-south zone of both ductile and brittle normal faulting along the southern margin of the Brooks Range probably formed in the mid-Cretaceous by extensional exhumation of the Early Cretaceous contractional deformation. copyright. Published in 1997 by the American Geophysical Union.

  10. Distribution characteristics and influencing factors of soil organic carbon in alpine ecosystems on the Tibetan Plateau transect, China

    Institute of Scientific and Technical Information of China (English)

    Yuqiang TIAN; Hua OUYANG; Minghua SONG; Haishan NIU; Qiwu HU

    2008-01-01

    The undisturbed regions along the Qinghai-Tibet Highway crossing the natural zones of montane desert, alpine meadow-steppe, and montane shrub-conifer-ous forest were chosen as the study areas. Soil samples were collected at 23 sites and the relations between the influ-encing factors and distribution of soil organic carbon (SOC) content were studied. The results indicated that the order of SOC content for the whole soil profile with differ-ent vegetations and in the horizontal direction was shown as below: forest > shrub > meadow > steppe > desert. All the SOC contents of the top 10 cm soil layers of forest, shrub and meadow vegetations, as well as that of the top 20 cm soil layers in steppe, in the vertical direction, were higher than those of corresponding lower soil layers. However, the SOC content in the desert soil was in accord-ance. The grey correlative analysis between the climatic factors and SOC content in the top soil show that precip-itation was the dominant climatic factor affecting the dis-tribution of SOC in the Tibetan Plateau transect. The influence of precipitation on the horizontal distribution of SOC decreased with the increase of precipitation in the horizontal direction. The vertical distribution of SOC along the soil profile was greatly affected by precipitation or the soil clay content in top soil layers, and was clearly influ-enced by soil silt content or sand content in lower soil layers, as well. The influences of both soil bulk density and soil pH on the vertical distribution of SOC along the soil profile gradually declined. The plant biomass was the most import-ant biotic factors affecting the distribution of the SOC.

  11. Prevention of Cartilage Degeneration and Restoration of Chondroprotection by Lubricin Tribosupplementation in the Rat Following ACL Transection

    Science.gov (United States)

    Jay, Gregory D.; Fleming, Braden C.; Watkins, Bryn A.; McHugh, Karen A.; Anderson, Scott C.; Zhang, Ling X.; Teeple, Erin; Waller, Kimberly A.; Elsaid, Khaled A.

    2010-01-01

    Objective To investigate whether cartilage degeneration is prevented or minimized in an anterior cruciate ligament (ACL) injury rat model following intra-articular injections of lubricin derived from human synoviocytes in culture (HSL), recombinant protein (rhPRG4), or from human synovial fluids (HSFL). Methods Unilateral ACL transection (ACLT) was performed in Lewis rats (n=45). Intra-articular injections (50μl/injection) of PBS (n=9), HSL (n=9; 200μg/ml), rhPRG4 (n=9; 200μg/ml) and HSFL (n=9; 200μg/ml) started on day 7 post-injury and continued twice weekly. Animals were harvested on day 32 post-injury. Histological analysis was performed using Safranin O/Fast green stain and blinded investigators graded articular cartilage degeneration using OARSI modified Mankin criteria. Histological specimens were immunoprobed for lubricin and sulphated glycosaminoglycans. 24 hour urine collection was performed on days 17 and 29 post-injury and urinary CTXII (uCTXII) levels were measured. Results Treatment with HSL resulted in significantly (p<0.05) lower OARSI scores for cartilage degeneration compared to no treatment or PBS treatment. Increased immunostaining for lubricin in the superficial zone chondrocytes and on the surface of cartilage was observed in lubricins-treated but not untreated or PBS-treated joints. On day 17, uCTXII levels of HSL and HSFL-treated animals were significantly lower than untreated (p=0.005; p=0.002) and PBS-treated (p=0.002; p<0.001) animals, respectively. Conclusion Across all types of lubricin evaluated in this study, a reduction in cartilage damage following ACLT was evident, combined with a reduction in collagen type II degradation. Intraarticular lubricin injection following an ACL injury may be beneficial in retarding cartilage degeneration and development of post-traumatic OA. PMID:20506144

  12. Decreased gastric emptying and gastrointestinal and intestinal transits of liquid after complete spinal cord transection in awake rats

    Directory of Open Access Journals (Sweden)

    Gondim F. de-A.A.

    1998-01-01

    Full Text Available We studied the effect of complete spinal cord transection (SCT on gastric emptying (GE and on gastrointestinal (GI and intestinal transits of liquid in awake rats using the phenol red method. Male Wistar rats (N = 65 weighing 180-200 g were fasted for 24 h and complete SCT was performed between C7 and T1 vertebrae after a careful midline dorsal incision. GE and GI and intestinal transits were measured 15 min, 6 h or 24 h after recovery from anesthesia. A test meal (0.5 mg/ml phenol red in 5% glucose solution was administered intragastrically (1.5 ml and the animals were sacrificed by an iv thiopental overdose 10 min later to evaluate GE and GI transit. For intestinal transit measurements, 1 ml of the test meal was administered into the proximal duodenum through a cannula inserted into a gastric fistula. GE was inhibited (P<0.05 by 34.3, 23.4 and 22.7%, respectively, at 15 min, 6 h and 24 h after SCT. GI transit was inhibited (P<0.05 by 42.5, 19.8 and 18.4%, respectively, at 15 min, 6 h and 24 h after SCT. Intestinal transit was also inhibited (P<0.05 by 48.8, 47.2 and 40.1%, respectively, at 15 min, 6 h and 24 h after SCT. Mean arterial pressure was significantly decreased (P<0.05 by 48.5, 46.8 and 41.5%, respectively, at 15 min, 6 h and 24 h after SCT. In summary, our report describes a decreased GE and GI and intestinal transits in awake rats within the first 24 h after high SCT.

  13. 24R,25-Dihydroxyvitamin D3 Protects against Articular Cartilage Damage following Anterior Cruciate Ligament Transection in Male Rats

    Science.gov (United States)

    Boyan, Barbara D.; Hyzy, Sharon L.; Pan, Qingfen; Scott, Kayla M.; Coutts, Richard D.; Healey, Robert; Schwartz, Zvi

    2016-01-01

    Osteoarthritis (OA) in humans is associated with low circulating 25-hydroxyvitamin D3 [25(OH)D3]. In vitamin D replete rats, radiolabeled 24R,25-dihydroxyvitamin D3 [24R,25(OH)2D3] accumulates in articular cartilage following injection of [3H]-25(OH)D3. Previously, we showed that 24R,25(OH)2D3 blocks chondrocyte apoptosis via phospholipase D and p53, suggesting a role for 24R,25(OH)2D3 in maintaining cartilage health. We examined the ability of 24R,25(OH)2D3 to prevent degenerative changes in articular cartilage in an OA-like environment and the potential mechanisms involved. In vitro, rat articular chondrocytes were treated with IL-1β with and without 24R,25(OH)2D3 or 1α,25(OH)2D3. 24R,25(OH)2D3 but not 1α,25(OH)2D3 blocked the effects of IL-1β in a dose-dependent manner, and its effect was partially mediated through the TGF-β1 signaling pathway. In vivo, unilateral anterior cruciate ligament transections were performed in immunocompetent rats followed by intra-articular injections of 24R,25(OH)2D3 or vehicle (t = 0, 7, 14, 21 days). Tissues were harvested on day 28. Joints treated with vehicle had changes typical of OA whereas joints treated with 24R,25(OH)2D3 had less articular cartilage damage and levels of inflammatory mediators. These results indicate that 24R,25(OH)2D3 protects against OA, and suggest that it may be a therapeutic approach for preventing trauma-induced osteoarthritis. PMID:27575371

  14. Estimation of wind erosion rates by using 137Cs tracing technique: A case study in Tariat-Xilin Gol transect, Monsolian Plateau

    Institute of Scientific and Technical Information of China (English)

    LIU JiYuan; QI YongQing; SHI HuaDing; ZHUANG DaFang; HU YunFeng

    2008-01-01

    Wind erosion is one of the major environmental problems in semi-arid and arid regions. Here we established the Tariat-Xilin Gol transect from northwest to southeast across the Mongolian Plateau, and selected seven sampling sites along the transect. We then estimated the soil wind erosion rates by using the 137Cs tracing technique and examined their spatial dynamics. Our results showed that the (from Tariat to Sainshand), the wind erosion rate increased gradually with vegetation type and climatic regimes; the wind erosion process was controlled by physical factors such as annual precipitation and vegetation coverage, etc., and the impact of human activities was negligible. While in the China section of the transect (Inner Mongolia), the wind erosion rates of Xilin Hot and Zhengxiangbai Banner were thrice as much as those of Bayannur of Mongolia, although these three sites were all dominated by typical steppe. Besides the physical factors, higher population density and livestock carrying level should be responsible for the higher wind erosion rates in these two regions of Inner Mongolia.

  15. Rapid automated nuclear chemistry

    Energy Technology Data Exchange (ETDEWEB)

    Meyer, R.A.

    1979-05-31

    Rapid Automated Nuclear Chemistry (RANC) can be thought of as the Z-separation of Neutron-rich Isotopes by Automated Methods. The range of RANC studies of fission and its products is large. In a sense, the studies can be categorized into various energy ranges from the highest where the fission process and particle emission are considered, to low energies where nuclear dynamics are being explored. This paper presents a table which gives examples of current research using RANC on fission and fission products. The remainder of this text is divided into three parts. The first contains a discussion of the chemical methods available for the fission product elements, the second describes the major techniques, and in the last section, examples of recent results are discussed as illustrations of the use of RANC.

  16. JINR rapid communications

    International Nuclear Information System (INIS)

    The present collection of rapid communications from JINR, DUBNA, contains eight separate records on symmetry in modern physics (dedicated to the 100th anniversary of the birth of academician V.A.Fock), the double φ-meson production investigation on the Serpukhov accelerator, two-leptonic η-meson decays and SUSY without R parity, charge form factors and alpha-cluster internal structure of 12C, increasing of muon-track reconstruction efficiency in ME1/1 Dubna prototype for the CMS/LHC, study of photon-structure function F2γ in the reaction e+e- → e+e- + hadrons at LEP2, jets reconstruction possibility in pAu and AuAu interactions at STAR RHIC and high-vacuum nondispersable gas absorber

  17. JINR rapid communications

    International Nuclear Information System (INIS)

    The present collection of rapid communications from JINR, Dubna, contains twelve separate records on pseudoscalar form factor from inverse pion electroproduction and the first radial pion excitation, chiral and parity anomalies at finite temperature and density, the canonical form of an elastoplastic model of nuclear fusion, the peculiarities of the reactions of heavy and superheavy element synthesis within the dinuclear system concept, a study of the photon structure function F2γ in the reaction e+e-→e+e- + hadrons at LEP1 and LEP2, tritons for the study of the charge-exchange reactions with the LHE streamer chamber: status and some possibilities, nonlinear dynamics in nuclotron, current transformers and optical diagnostic monitor of nuclotron beam, the design features of the LHE JINR nuclotron internal target stations, a system of graphics digitization (SGD), numeric simulation of thermal treatment of metal surface by means of high current ion beam

  18. JINR rapid communications

    International Nuclear Information System (INIS)

    The present collection of rapid communications from JINR, Dubna, contains nine separate reports on effects arising from charged particles overcoming of the light velocity barrier, deformable templates for circle recognition, scintillation detectors for precise time measurements, atomic form factors and incoherent scattering functions of atoms and ions with the number of electrons N ≤ 10, experimental set-up ANOMALON for measurement of relativistic nuclear fragmentation cross sections, superconducting dipole magnet for ALICE dimuon arm spectrometer, analysis of transverse mass dependence of Bose-Einstein correlation radii using the DELPHI data, low-energy theorem in softly broken supersymmetry and study of the characteristics of particles in reactions π-, p, d, He, C + C with the total disintegration on carbon nucleus

  19. JINR rapid communications

    International Nuclear Information System (INIS)

    The present collection of rapid communications from JINR, Dubna, contains seven separate records on measurements of the total cross section difference ΔσL(np) at 1.59, 1.79, and 2.20 GeV, to the estimation of angular distributions of double charged spectator fragments in nucleus-nucleus interactions at superhigh energies, simulation dE/dx analysis results for silicon inner tracking system of ALICE set-up at LHC accelerator, high-multiplicity processes, triggering of high-multiplicity events using calorimetry, ORBIT-3.0 - a computer code for simulation and correction of the closed orbit and first turn in synchrotrons and determination of memory performance

  20. Higgsstrahlung at forward rapidities

    CERN Document Server

    Pasechnik, Roman; Potashnikova, Irina

    2014-01-01

    We discuss the inclusive and single diffractive heavy flavor (top and bottom) production in association with the Higgs boson at forward rapidities in proton-proton collisions at the LHC. The calculations are performed in the framework of the phenomenological dipole approach, which automatically accounts for the absorptive corrections induced by soft interactions, i.e. for the gap survival effects at the amplitude level. Major differential observables including the realistic ATLAS detector constraints are considered. The forward inclusive and diffractive Higgsstrahlung processes are generated essentially by excitation of the valence or sea quarks in the proton. The single diffractive Higgsstrahlung off top quarks is found to dominate compared to the loop-induced mechanism at sufficiently large Higgs boson transverse momenta. The Higgsstrahlung processes offer a direct and precise measurement of Higgs-top and, possibly, Higgs-bottom Yukawa couplings at the LHC, as well as the studies of the intrinsic heavy flav...

  1. Rapid Diagnosis of Malaria

    Directory of Open Access Journals (Sweden)

    Clinton K. Murray

    2009-01-01

    Full Text Available Malaria's global impact is expansive and includes the extremes of the healthcare system ranging from international travelers returning to nonendemic regions with tertiary referral medical care to residents in hyperendemic regions without access to medical care. Implementation of prompt and accurate diagnosis is needed to curb the expanding global impact of malaria associated with ever-increasing antimalarial drug resistance. Traditionally, malaria is diagnosed using clinical criteria and/or light microscopy even though both strategies are clearly inadequate in many healthcare settings. Hand held immunochromatographic rapid diagnostic tests (RDTs have been recognized as an ideal alternative method for diagnosing malaria. Numerous malaria RDTs have been developed and are widely available; however, an assortment of issues related to these products have become apparent. This review provides a summary of RDT including effectiveness and strategies to select the ideal RDT in varying healthcare settings.

  2. Rapid automated nuclear chemistry

    International Nuclear Information System (INIS)

    Rapid Automated Nuclear Chemistry (RANC) can be thought of as the Z-separation of Neutron-rich Isotopes by Automated Methods. The range of RANC studies of fission and its products is large. In a sense, the studies can be categorized into various energy ranges from the highest where the fission process and particle emission are considered, to low energies where nuclear dynamics are being explored. This paper presents a table which gives examples of current research using RANC on fission and fission products. The remainder of this text is divided into three parts. The first contains a discussion of the chemical methods available for the fission product elements, the second describes the major techniques, and in the last section, examples of recent results are discussed as illustrations of the use of RANC

  3. Rapid shallow breathing index.

    Science.gov (United States)

    Karthika, Manjush; Al Enezi, Farhan A; Pillai, Lalitha V; Arabi, Yaseen M

    2016-01-01

    Predicting successful liberation of patients from mechanical ventilation has been a focus of interest to clinicians practicing in intensive care. Various weaning indices have been investigated to identify an optimal weaning window. Among them, the rapid shallow breathing index (RSBI) has gained wide use due to its simple technique and avoidance of calculation of complex pulmonary mechanics. Since its first description, several modifications have been suggested, such as the serial measurements and the rate of change of RSBI, to further improve its predictive value. The objective of this paper is to review the utility of RSBI in predicting weaning success. In addition, the use of RSBI in specific patient populations and the reported modifications of RSBI technique that attempt to improve the utility of RSBI are also reviewed. PMID:27512505

  4. Rapid chemical separations

    CERN Document Server

    Trautmann, N

    1976-01-01

    A survey is given on the progress of fast chemical separation procedures during the last few years. Fast, discontinuous separation techniques are illustrated by a procedure for niobium. The use of such techniques for the chemical characterization of the heaviest known elements is described. Other rapid separation methods from aqueous solutions are summarized. The application of the high speed liquid chromatography to the separation of chemically similar elements is outlined. The use of the gas jet recoil transport method for nuclear reaction products and its combination with a continuous solvent extraction technique and with a thermochromatographic separation is presented. Different separation methods in the gas phase are briefly discussed and the attachment of a thermochromatographic technique to an on-line mass separator is shown. (45 refs).

  5. Rapid prototyping and time compression

    OpenAIRE

    Chatwin, Chris

    2000-01-01

    Rapid Prototyping - manufacture by layering processes: Stereolithography Selective Layer Sintering (SLS) Laminated Object Manufacture (LOM) Solid Ground Curing Small batch programmable rapid manufacture with lasers Micro-Engineering – Prototyping and Manufacture Additive - fabrication Subtractive – machining

  6. Rapid Refresh (RAP) [20 km

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Rapid Refresh (RAP) numerical weather model took the place of the Rapid Update Cycle (RUC) on May 1, 2012. Run by the National Centers for Environmental...

  7. Rapid Refresh (RAP) [13 km

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Rapid Refresh (RAP) numerical weather model took the place of the Rapid Update Cycle (RUC) on May 1, 2012. Run by the National Centers for Environmental...

  8. Rapid geophysical surveyor

    International Nuclear Information System (INIS)

    The Rapid Geophysical Surveyor (RGS) is a system designed to rapidly and economically collect closely-spaced geophysical data used for characterization of Department of Energy (DOE) waste sites. Geophysical surveys of waste sites are an important first step in the remediation and closure of these sites; especially older sties where historical records are inaccurate and survey benchmarks have changed due to refinements in coordinate controls and datum changes. Closely-spaced data are required to adequately differentiate pits, trenches, and soil vault rows whose edges may be only a few feet from each other. A prototype vehicle designed to collect magnetic field data was built at the Idaho national Engineering Laboratory (INEL) during the summer of 1992. The RGS was one of several projects funded by the Buried Waste Integrated Demonstration (BWID) program. This vehicle was demonstrated at the Subsurface Disposal Area (SDA) within the Radioactive Waste Management Complex (RWMC) on the INEL in September of 1992. Magnetic data were collected over two areas in the SDA, with a total survey area of about 1.7 acres. Data were collected at a nominal density of 2 1/2 inches along survey lines spaced 1 foot apart. Over 350,000 data points were collected over a 6 day period corresponding to about 185 man-days using conventional ground survey techniques. This report documents the design and demonstration of the RGS concept including the presentation of magnetic data collected at the SDA. The surveys were able to show pit and trench boundaries and determine details of their spatial orientation never before achieved

  9. Geochemistry of soils along a transect from Central Mexico to the Pacific Coast: a pilot study for continental-scale geochemical mapping

    Science.gov (United States)

    Chiprés, J.A.; de la Calleja,; Tellez, J.I.; Jiménez, F.; Cruz, Carlos; Guerrero, E.G.; Castro, J.; Monroy, M.G.; Salinas, J.C.

    2009-01-01

    The Mexican Geological Survey (SGM), the National Institute of Statistics, Geography and Informatics (INEGI) and the Autonomous University of San Luis Potosi (UASLP) have established a multidisciplinary team with the objective of creating a national program of geochemical mapping of soils in Mexico. This is being done as part of the North American Soil Geochemical Landscapes Project in partnership with the US Geological Survey and the Geological Survey of Canada. As the first step, a pilot study was conducted over a transect that extends from the Mexico–US border near Ciudad Juarez in the north to the Pacific Ocean in the south. This pilot transect was conducted in two phases, and this paper presents results from the first phase, which sampled soils at about a 40-km spacing along a 730-km transect beginning in Central Mexico and ending at the Pacific Coast. Samples were collected from the A and C horizons at each site and 60 elements were analyzed. This pilot study demonstrates that geochemical mapping based on a 40-km spacing is adequate to identify broad-scale geochemical patterns. Geologic influence (i.e., soil parent material) was the most important factor influencing the distribution of elements along the transect, followed by the influence of regional mineralization. The study also showed that influence by human activities over the transect is minimal except possibly in large mining districts. A comparison of element abundance in the A horizon with the environmental soil guidelines in Mexico showed that the natural concentrations of the studied soils were lower than the established threshold for soil restoration with the exception of V and As. The former had a median value (75 mg/kg) approximately equal to the value established in Mexico for soil restoration in agricultural and residential lands (78 mg/kg), and the latter had three values higher than the 22 mg/kg threshold for soil restoration in agricultural and residential lands. These cases demonstrate

  10. Evaluation of Scat Deposition Transects versus Radio Telemetry for Developing a Species Distribution Model for a Rare Desert Carnivore, the Kit Fox.

    Directory of Open Access Journals (Sweden)

    Steven J Dempsey

    Full Text Available Development and evaluation of noninvasive methods for monitoring species distribution and abundance is a growing area of ecological research. While noninvasive methods have the advantage of reduced risk of negative factors associated with capture, comparisons to methods using more traditional invasive sampling is lacking. Historically kit foxes (Vulpes macrotis occupied the desert and semi-arid regions of southwestern North America. Once the most abundant carnivore in the Great Basin Desert of Utah, the species is now considered rare. In recent decades, attempts have been made to model the environmental variables influencing kit fox distribution. Using noninvasive scat deposition surveys for determination of kit fox presence, we modeled resource selection functions to predict kit fox distribution using three popular techniques (Maxent, fixed-effects, and mixed-effects generalized linear models and compared these with similar models developed from invasive sampling (telemetry locations from radio-collared foxes. Resource selection functions were developed using a combination of landscape variables including elevation, slope, aspect, vegetation height, and soil type. All models were tested against subsequent scat collections as a method of model validation. We demonstrate the importance of comparing multiple model types for development of resource selection functions used to predict a species distribution, and evaluating the importance of environmental variables on species distribution. All models we examined showed a large effect of elevation on kit fox presence, followed by slope and vegetation height. However, the invasive sampling method (i.e., radio-telemetry appeared to be better at determining resource selection, and therefore may be more robust in predicting kit fox distribution. In contrast, the distribution maps created from the noninvasive sampling (i.e., scat transects were significantly different than the invasive method, thus scat

  11. NOAA Line Shapefile- Locations of Phantom S2 ROV Underwater Video Transects, US Virgin Islands, Project NF-05-05, 2005, UTM 20N WGS84 (NCEI Accession 0131860)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This dataset contains a line shapefile showing the trackline of various Remotely Operated Vehicle (ROV) underwater video transects in the US Virgin Islands.NOAA's...

  12. Chemistry of surface sediment along a north-south transect across the equator in the Central Indian Basin: An assessment of biogenic and detrital influences on elemental burial on the seafloor

    Digital Repository Service at National Institute of Oceanography (India)

    Banakar, V.K.; Parthiban, G.; Pattan, J.N.; Jauhari, P.

    The detailed chemistry of the surface sediment collected along a north-south transect across the equator in the Central Indian Ocean suggest that: biogenic sedimentation north of equator is dominated by carbonate ooze and the south of equator...

  13. Cetacean line-transect survey conducted in the eastern Bering Sea shelf by Alaska Fisheries Science Center, National Marine Mammal Laboratory from NOAA Ship Miller Freeman from 1999-07-07 to 2004-06-30 (NCEI Accession 0131862)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Visual surveys for cetaceans were conducted on the eastern Bering Sea shelf along transect lines, in association with the AFSC’s echo integration trawl surveys...

  14. Oceanographic data collected during the Estuary to the Abyss: Exploring Along the Latitude 31-30 Transect (abyss2004) on RV Seward Johnson in Charleston Bump from August 19, 2004 - September 1, 2004 (NODC Accession 0072309)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The diverse ocean-bottom habitats off the coast of South Carolina and Georgia support a corresponding diversity of organisms. An imaginary line, or transect,...

  15. Transects used for pre- and postconstruction data collection at constructed emergent sandbar habitats downstream from Gavins Point Dam near River Miles 761.4 and 769.8 on the Missouri River

    Data.gov (United States)

    U.S. Geological Survey, Department of the Interior — This dataset represents the transects used for hydrographic surveys of Emergent Sandbar Habitat at two sites near river miles 761.4 and 769.8 downstream of Gavins...

  16. The Rapid Transient Surveyor

    Science.gov (United States)

    Baranec, Christoph; Tonry, John; Wright, Shelley; Tully, R. Brent; Lu, Jessica R.; Takamiya, Marianne Y.; Hunter, Lisa

    2016-01-01

    The next decade of astronomy will be dominated by large area surveys (see the detailed discussion in the Astro-2010 Decadal survey and NRC's recent OIR System Report). Ground-based optical transient surveys, e.g., LSST, ZTF and ATLAS and space-based exoplanet, supernova, and lensing surveys such as TESS and WFIRST will join the Gaia all-sky astrometric survey in producing a flood of data that will enable leaps in our understanding of the universe. There is a critical need for further characterization of these discoveries through high angular resolution images, deeper images, spectra, or observations at different cadences or periods than the main surveys. Such follow-up characterization must be well matched to the particular surveys, and requires sufficient additional observing resources and time to cover the extensive number of targets.We describe plans for the Rapid Transient Surveyor (RTS), a permanently mounted, rapid-response, high-cadence facility for follow-up characterization of transient objects on the U. of Hawai'i 2.2-m telescope on Maunakea. RTS will comprise an improved robotic laser adaptive optics system, based on the prototype Robo-AO system (formerly at the Palomar 1.5-m and now at the Kitt Peak 2.2-m telescope), with simultaneous visible and near-infrared imagers as well as a near-infrared integral field spectrograph (R~100, λ = 850 - 1830 nm, 0.15″ spaxels, 8.7″×6.0″ FoV). RTS will achieve an acuity of ~0.07″ in visible wavelengths and < 0.16″ in the near infrared leading to an increase of the infrared point-source sensitivity against the sky background by a factor of ~9, crucial for efficient near-infrared spectroscopy.RTS will allow us to map the dark matter distribution in the z < 0.1 local universe with ten times better accuracy and precision than previous experiments. ATLAS will discover several thousand SNIae per year, measuring SNIa peak brightness, and decline rates, while RTS will measure reddening by dust, confirm SN type and

  17. Combining satellite and seismic images to analyse the shallow structure of the Dead Sea Transform near the DESERT transect

    Science.gov (United States)

    Kesten, D.; Weber, M.; Haberland, Ch.; Janssen, Ch.; Agnon, A.; Bartov, Y.; Rabba, I.

    2008-02-01

    The left-lateral Dead Sea Transform (DST) in the Middle East is one of the largest continental strike-slip faults of the world. The southern segment of the DST in the Arava/Araba Valley between the Dead Sea and the Red Sea, called Arava/Araba Fault (AF), has been studied in detail in the multidisciplinary DESERT (DEad SEa Rift Transect) project. Based on these results, here, the interpretations of multi-spectral (ASTER) satellite images and seismic reflection studies have been combined to analyse geologic structures. Whereas satellite images reveal neotectonic activity in shallow young sediments, reflection seismic image deep faults that are possibly inactive at present. The combination of the two methods allows putting some age constraint on the activity of individual fault strands. Although the AF is clearly the main active fault segment of the southern DST, we propose that it has accommodated only a limited (up to 60 km) part of the overall 105 km of sinistral plate motion since Miocene times. There is evidence for sinistral displacement along other faults, based on geological studies, including satellite image interpretation. Furthermore, a subsurface fault is revealed ≈4 km west of the AF on two ≈E-W running seismic reflection profiles. Whereas these seismic data show a flower structure typical for strike-slip faults, on the satellite image this fault is not expressed in the post-Miocene sediments, implying that it has been inactive for the last few million years. About 1 km to the east of the AF another, now buried fault, was detected in seismic, magnetotelluric and gravity studies of DESERT. Taking together various evidences, we suggest that at the beginning of transform motion deformation occurred in a rather wide belt, possibly with the reactivation of older ≈N-S striking structures. Later, deformation became concentrated in the region of today’s Arava Valley. Till ≈5 Ma ago there might have been other, now inactive fault traces in the vicinity

  18. Nitrogen fixation in sediments along a depth transect through the Eastern Boundary Upwelling Systems off Peru and Mauritania

    Science.gov (United States)

    Gier, J.; Sommer, S.; Löscher, C. R.; Dale, A.; Schmitz, R. A.; Treude, T.

    2015-12-01

    The distribution of benthic nitrogen (N2) fixation and its relevance for N cycling in the Eastern Boundary Upwelling Systems (EBUS) are still unknown. Recent studies confirm that benthic N2 fixation can be coupled to sulfate reduction (SR) and that several species of sulfate reducing bacteria have the genetic ability to fix N due to the presence of the gene encoding for the nitrogenase enzyme. We investigated benthic N2 fixation and SR in the Peruvian oxygen minimum zone at 12°S and in the Mauritanian upwelling system at 18°N along a depth transect. Sediments were retrieved by a multicorer and a benthic lander at six stations in both regions. Benthic N2 fixation occurred throughout the sediment in both EBUS. Off Peru the highest integrated (0-20 cm) N2 fixation rate of 0.4 mmol N/m2/d was measured inside the core of the OMZ at 253 m water depth. Off Mauritania the highest integrated (0-20 cm) N2 fixation rate of 0.15 mmol N/m2/d was measured at 90 m, coinciding with a low bottom water oxygen concentration (30 μM). N2 fixation depth profiles often overlapped with SR activity. Moreover, sequencing data yielded insights into the composition and diversity of the nifH gene pool in EBUS sediments. Interestingly, detected sequences in both EBUS clustered with SR bacteria, such as Desulfovibrio vulgaris and several of the novel detected clades belonged to uncultured diazotrophs. Our results suggest that N2 fixation and SR were coupled to a large extent in both regions. However, potential environmental factors controlling benthic diazotrophs in the EBUS appear to be the availability of sulfide and organic matter. Additionally, no inhibition of N2 fixation at high ammonium concentrations was found, which highlights gaps in our knowledge regards the interaction between ammonium availability and diazotrophy. Our results contribute to a better understanding of N cycling in EBUS sediments and sources of fixed N.

  19. The effect of minocycline on the masticatory movements following the inferior alveolar nerve transection in freely moving rats

    Directory of Open Access Journals (Sweden)

    Mostafeezur Rahman

    2012-04-01

    Full Text Available Abstract Background To determine the effects of inferior alveolar nerve transection (IAN-X on masticatory movements in freely moving rats and to test if microglial cells in the trigeminal principal sensory nucleus (prV or motor nucleus (motV may be involved in modulation of mastication, the effects of microglial cell inhibitor minocycline (MC on masticatory jaw movements, microglia (Iba1 immunohistochemistry and the masticatory jaw movements and related masticatory muscle EMG activities were studied in IAN-X rats. Results The number of Iba1-immunoreactive (IR cells both in prV and motV was significantly larger in IAN-X rats compared with sham rats on day 3 after IAN-X. The intraperitoneal (i.p. administration of MC caused a significant reduction of the number of Iba1-IR cells both in prV and motV that was evident on day 14 after IAN-X. Furthermore, a significant reduction of the number of Iba1-IR cells could be observed in motV but not in prV after microinjection (m.i. of MC into the motV of IAN-X rats. The rats also exhibited a significant decrease in the head-withdrawal threshold on the side ipsilateral to the IAN-X compared to the threshold before IAN-X and it lasted to day 14. In addition, IAN-X markedly affected the ability to rat to carry out mastication. The number of complete masticatory sequences was significantly decreased. Furthermore, the total masticatory sequence time and food preparatory (PP period duration was significantly elongated in compared to sham rats. Although IAN-X significantly affected the total number of chewing cycles within the RC period of a masticatory sequence, it had no effect on the duration of the chewing cycles. On the other hand, systemic administration of MC (both i.p. and m.i. in IAN-X rats significantly improved decreased head-withdrawal threshold and the impaired masticatory jaw movements. Conclusions The present findings reveal that the strong modulation of masticatory jaw movements occurs following

  20. Cervical sympathetic trunk transection affects inducible nitric oxide synthase expression in rat hippocampus following focal cerebral ischemia/reperfusion injury

    Institute of Scientific and Technical Information of China (English)

    Liangzhi Xiong; Yan Wang; Qingxiu Wang; Qingshan Zhou

    2008-01-01

    BACKGROUND: The stellate ganglion block (SGB) plays a protective role in focal cerebral ischemia/reperfusion injury. The human SGB can be simulated by transection of the cervical sympathetic trunk (TCST) in rats.OBJECTIVE: To observe the effects of TCST on inducible nitric oxide synthase (iNOS) levels and cerebral infarct wolume in the hippocampus of rats with cerebral ischemia/reperfusion injury, and to analyze the mechanism of action.DESIGN, TIME AND SETTING: A completely randomized, controlled, neuropathological experiment was performed at the Institute of Neurological Disease, Taihe Hospital, Yunyang Medical College between March and September 2006.MATERIALS: A total of 93 Wistar rats, aged 17-18 weeks, of either gender, were used for this study. 2, 3, 5-triphenyl tetrazolium chloride was purchased from Changsha Hongyuan Biological Reagent Company, China. Rabbit iNOS antibody and goat anti-rabbit lgG antibody were the products of Wuhan Boster Biological Reagent Co., Ltd., China.METHODS: Ten rats were randomly selected for the sham-operated group. Cerebral ischemia/reperfusion injury was induced by middle cerebral artery occlusion (MCAO) using the suture method in the remaining rats. Forty successful rat models were randomly and equally divided into the following two groups: (1) TCST group: subsequent to TCST, MCAO was performed for 2 hours, followed by 24 hours reperfusion; (2) model group: rats underwent experimental procedures similar to the TCST group, with the exception of TCST. Rats in the sham-operated group were subjected to experimental procedures similar to the model group; however, the thread was only introduced to a depth of 10 mm.MAIN OUTCOME MEASURES: Following 24 hours of reperfusion, functional neurological deficits were scored. Brain tissue sections from ten rats of each group were used to measure cerebral infarct volume by TTC staining. Hippocampal tissue sections of an additional ten rats from each group were used to detect iNOS levels using

  1. Cenozoic global sea level, sequences, and the New Jersey transect: Results from coastal plain and continental slope drilling

    Science.gov (United States)

    Miller, K.G.; Mountain, Gregory S.; Browning, J.V.; Kominz, M.; Sugarman, P.J.; Christie-Blick, N.; Katz, M.E.; Wright, J.D.

    1998-01-01

    The New Jersey Sea Level Transect was designed to evaluate the relationships among global sea level (eustatic) change, unconformity-bounded sequences, and variations in subsidence, sediment supply, and climate on a passive continental margin. By sampling and dating Cenozoic strata from coastal plain and continental slope locations, we show that sequence boundaries correlate (within ??0.5 myr) regionally (onshore-offshore) and interregionally (New Jersey-Alabama-Bahamas), implicating a global cause. Sequence boundaries correlate with ??18O increases for at least the past 42 myr, consistent with an ice volume (glacioeustatic) control, although a causal relationship is not required because of uncertainties in ages and correlations. Evidence for a causal connection is provided by preliminary Miocene data from slope Site 904 that directly link ??18O increases with sequence boundaries. We conclude that variation in the size of ice sheets has been a primary control on the formation of sequence boundaries since ~42 Ma. We speculate that prior to this, the growth and decay of small ice sheets caused small-amplitude sea level changes (sea level amplitudes are substantially lower than theirs. Lithofacies patterns within sequences follow repetitive, predictable patterns: (1) coastal plain sequences consist of basal transgressive sands overlain by regressive highstand silts and quartz sands; and (2) although slope lithofacies variations are subdued, reworked sediments constitute lowstand deposits, causing the strongest, most extensive seismic reflections. Despite a primary eustatic control on sequence boundaries, New Jersey sequences were also influenced by changes in tectonics, sediment supply, and climate. During the early to middle Eocene, low siliciclastic and high pelagic input associated with warm climates resulted in widespread carbonate deposition and thin sequences. Late middle Eocene and earliest Oligocene cooling events curtailed carbonate deposition in the coastal

  2. Rapidly Evolving Giant Dermatofibroma

    Directory of Open Access Journals (Sweden)

    K. J. Lang

    2010-01-01

    Full Text Available Dermatofibroma, also known as “fibrous histiocytoma”, is a benign dermal or subcutaneous poorly circumscribed proliferation of spindle-shaped fibroblasts and macrophages in the dermis. Although it is commonly present as a brownish nodule the legs of females, it may also arise on the upper extremities, trunk, and rarely on the head. The exact pathogenesis is unclear. However, it is widely believed that the originating insult to the dermis is a folliculitis, an arthropod bite, or an unspecified initial inflammatory condition. Giant dermatofibromas of greater than 5 cm in diameter are rare, with only 22 cases reported in the literature. We present a case of a rapidly evolving pedunculated mass in the groin of a male patient. Histological examination confirmed this to be a giant dermatofibroma. Though this specimen cannot is not confirmed as such, the cellular subtype is sometimes present as a larger lesion with anecdotal reports of local recurrence and distant metastases. The clinical and radiological features which were somewhat suspicious of malignancy are considered in the context of the definitive pathological diagnosis of a benign lesion.

  3. Rapid Polymer Sequencer

    Science.gov (United States)

    Stolc, Viktor (Inventor); Brock, Matthew W (Inventor)

    2013-01-01

    Method and system for rapid and accurate determination of each of a sequence of unknown polymer components, such as nucleic acid components. A self-assembling monolayer of a selected substance is optionally provided on an interior surface of a pipette tip, and the interior surface is immersed in a selected liquid. A selected electrical field is impressed in a longitudinal direction, or in a transverse direction, in the tip region, a polymer sequence is passed through the tip region, and a change in an electrical current signal is measured as each polymer component passes through the tip region. Each of the measured changes in electrical current signals is compared with a database of reference electrical change signals, with each reference signal corresponding to an identified polymer component, to identify the unknown polymer component with a reference polymer component. The nanopore preferably has a pore inner diameter of no more than about 40 nm and is prepared by heating and pulling a very small section of a glass tubing.

  4. Rapid Evaporation of microbubbles

    Science.gov (United States)

    Gautam, Jitendra; Esmaeeli, Asghar

    2008-11-01

    When a liquid is heated to a temperature far above its boiling point, it evaporates abruptly. Boiling of liquid at high temperatures can be explosive and destructive, and poses a potential hazard for a host of industrial processes. Explosive boiling may occur if a cold and volatile liquid is brought into contact with a hot and non-volatile liquid, or if a liquid is superheated or depressurized rapidly. Such possibilities are realized, for example, in the depressurization of low boiling point liquefied natural gas (LNG) in the pipelines or storage tanks as a result of a leak. While boiling of highly heated liquids can be destructive at macroscale, the (nearly) instantaneous pace of the process and the release of large amount of kinetic energy make the phenomena extremely attractive at microscale where it is possible to utilize the released energy to derive micromechanical systems. For instance, there is currently a growing interest in micro-explosion of liquid for generation of micro bubbles for actuation purposes. The aim of the current study is to gain a fundamental understanding of the subject using direct numerical simulations. In particular, we seek to investigate the boundary between stable and unstable nucleus growth in terms of the degree of liquid superheat and to compare the dynamics of unstable and stable growth.

  5. Rapidly rotating red giants

    CERN Document Server

    Gehan, Charlotte; Michel, Eric

    2016-01-01

    Stellar oscillations give seismic information on the internal properties of stars. Red giants are targets of interest since they present mixed modes, which behave as pressure modes in the convective envelope and as gravity modes in the radiative core. Mixed modes thus directly probe red giant cores, and allow in particular the study of their mean core rotation. The high-quality data obtained by CoRoT and Kepler satellites represent an unprecedented perspective to obtain thousands of measurements of red giant core rotation, in order to improve our understanding of stellar physics in deep stellar interiors. We developed an automated method to obtain such core rotation measurements and validated it for stars on the red giant branch. In this work, we particularly focus on the specific application of this method to red giants having a rapid core rotation. They show complex spectra where it is tricky to disentangle rotational splittings from mixed-mode period spacings. We demonstrate that the method based on the id...

  6. Northeast China Transect (NECT):Ten-Year Synthesis and Future Challenges%中国东北样带(NECT):十年集成与未来挑战

    Institute of Scientific and Technical Information of China (English)

    倪健; 王国宏

    2004-01-01

    Northeast China Transect (NECT), one of the fifteen International Biosphere-Geosphere Programme (IGBP) terrestrial transects, has been established for 10 years by Prof. Zhang Xin-Shi, through a core project of the IGBP - the Global Change and Terrestrial Ecosystems (GCTE). This transect is located in the mid-latitude semi-arid region, ranging 42-46° N latitude and 110-132°E longitude. The primary driving force for global change is precipitation and the secondary one is land use intensity.Research progresses have been performed during the past decade in the following aspects: ecological database development, climate and its variability, ecophysiological response of plants to environments,vegetation and landscape changes, biodiversity patterns and their changes, plant functional types and traits with relation to climatic gradient, productivity and carbon dynamics, pollen-vegetation relationship, trace gas emissions, land use and land cover changes, as well as biogeographical and biogeochemical modelling. In order to achieve the higher level of integrated research, the NECT needs the consistent basic data sets within the same framework, further field experiments and observations, integrated simulations of vegetation structure, process and function from patch, landscape to biome scales, intercomparisons of results and simulations within the transect and to other IGBP transects, multidisciplinary research,national and international co-ordinates, and full scientific plan and implementation strategy.%作为"国际地圈-生物圈计划(IGBP)"的15条陆地样带之一,中国东北样带(Northeast China Transect,NECT)在IGBP核心项目"全球变化与陆地生态系统(GCTE)"中已经建立10年之久.该样带位于中纬度温带半干旱地区,跨越北纬42~46°,东经110~132°,其主要全球变化驱动因素为降水,次要驱动因素为土地利用强度.在过去的10年里,中国东北样带的研究进展表现在以下几个方面:生态数据库发展

  7. A GEOBIA framework to estimate forest parameters from lidar transects, Quickbird imagery and machine learning: A case study in Quebec, Canada

    Science.gov (United States)

    Chen, Gang; Hay, Geoffrey J.; St-Onge, Benoît

    2012-04-01

    The GEOgraphic Object-Based Image Analysis (GEOBIA) paradigm continues to prove its efficacy in remote sensing image analysis by providing tools which emulate human perception and combine analyst's experience with meaningful image-objects. However, challenges remain in the evolution of this new paradigm as sophisticated methods attempt to deliver on the goal of automated geo-intelligence (i.e., geospatial content within context) from geospatial sources. In order to generate geo-intelligence from a forest scene, this article introduces a GEOBIA framework to estimate canopy height, above-ground biomass (AGB) and volume by combining lidar (light detection and ranging) transects, Quickbird imagery and machine learning algorithms. This framework is comprised three main components: (i) image-object extraction, (ii) lidar transect selection, and (iii) forest parameter generalization. The rational for integrating these methods is to provide a semi-automatic GEOBIA approach from which detailed forest information is obtained at the individual tree crown or small tree cluster level (i.e., mean object size of 0.04 ha); while also dramatically reducing airborne lidar data acquisition costs. Analysis is performed over a 16,330 ha forested study site in Quebec, Canada. Forest parameter estimation results derived from our GEOBIA framework demonstrate a strong relationship with those using the full lidar cover; where the highest estimates for canopy height ( R = 0.85; RMSE = 3.37 m), AGB ( R = 0.85; RMSE = 39.48 Mg/ha) and volume ( R = 0.85; RMSE = 52.59 m 3/ha) were achieved using a lidar transect sample representing only 7.6% of the total study area.

  8. Pb-concentrations and Pb-isotope ratios in soils collected along an east-west transect across the United States

    Science.gov (United States)

    Reimann, Clemens; Smith, David B.; Woodruff, Laurel G.; Flem, Belinda

    2011-01-01

    Analytical results for Pb-concentrations and isotopic ratios from ca. 150 samples of soil A horizon and ca. 145 samples of soil C horizon collected along a 4000-km east–west transect across the USA are presented. Lead concentrations along the transect show: (1) generally higher values in the soil A-horizon than the C-horizon (median 21 vs. 16.5 mg/kg), (2) an increase in the median value of the soil A-horizon for central to eastern USA (Missouri to Maryland) when compared to the western USA (California to Kansas) (median 26 vs. 20 mg/kg) and (3) a higher A/C ratio for the central to eastern USA (1.35 vs. 1.14). Lead isotopes show a distinct trend across the USA, with the highest 206Pb/207Pb ratios occurring in the centre (Missouri, median A-horizon: 1.245; C-horizon: 1.251) and the lowest at both coasts (e.g., California, median A-horizon: 1.195; C-horizon: 1.216). The soil C-horizon samples show generally higher 206Pb/207Pb ratios than the A-horizon (median C-horizon: 1.224; A-horizon: 1.219). The 206Pb/207Pb-isotope ratios in the soil A horizon show a correlation with the total feldspar content for the same 2500-km portion of the transect from east-central Colorado to the Atlantic coast that shows steadily increasing precipitation. No such correlation exists in the soil C horizon. The data demonstrate the importance of climate and weathering on both Pb-concentration and 206Pb/207Pb-isotope ratios in soil samples and natural shifts thereof in the soil profile during soil-forming processes.

  9. Heterotrophic prokaryote distribution along a 2300 km transect in the North Pacific subtropical gyre during strong La Niña conditions: relationship between distribution and hydrological conditions

    Directory of Open Access Journals (Sweden)

    M. Girault

    2014-11-01

    Full Text Available The spatial distribution of heterotrophic prokaryotes was investigated during the Tokyo–Palau cruise in the western part of the North Pacific subtropical gyre (NPSG along a north–south transect between 33.60 and 13.25° N. The cruise was conducted in three different hydrological areas identified as the Kuroshio region, the Subtropical gyre area and the Transition zone. Two eddies were crossed along the transect: one cold core cyclonic eddy and one warm core anticyclonic eddy and distributions of the heterotrophic prokaryotes were recorded. By using analytical flow cytometry and a nucleic acid staining protocol, heterotrophic prokaryotes were discriminated into three subgroups depending on their nucleic acid content (low, high and very high nucleic acid contents labeled LNA, HNA and VHNA, respectively. Statistical analyses performed on the dataset showed that LNA, mainly associated with temperature and salinity, were dominant in all the hydrological regions. In contrast, HNA distribution seemed to be associated with temperature, salinity, Chl a and silicic acid. A latitudinal increase in the HNA / LNA ratio was observed along the north–south transect and was related to higher phosphate and nitrate concentrations. In the Kuroshio Current, it is suggested that the high concentration of heterotrophic prokaryotes observed at station 4 was linked to the path of the cold cyclonic eddy core. In contrast, it is thought that low concentrations of heterotrophic prokaryotes in the warm core of the anticyclonic gyre (Sta. 9 are related to the low nutrient concentrations measured in the seawater column. Our results showed that the high variability between the various heterotrophic prokaryote cluster abundances depend both on the mesoscale structures and the oligotrophic gradient.

  10. Transformations of the chemical compositions of high molecular weight DOM along a salinity transect: Using two dimensional correlation spectroscopy and principal component analysis approaches

    Science.gov (United States)

    Abdulla, Hussain A. N.; Minor, Elizabeth C.; Dias, Robert F.; Hatcher, Patrick G.

    2013-10-01

    In a study of chemical transformations of estuarine high-molecular-weight (HMW, >1000 Da) dissolved organic matter (DOM) collected over a period of two years along a transect through the Elizabeth River/Chesapeake Bay system to the coastal Atlantic Ocean off Virginia, USA, δ13C values, N/C ratios, and principal component analysis (PCA) of the solid-state 13C NMR (nuclear magnetic resonance) spectra of HMW-DOM show an abrupt change in both its sources and chemical structural composition occurring around salinity 20. HMW-DOM in the lower salinity region had lighter isotopic values, higher aromatic and lower carbohydrate contents relative to that in the higher salinity region. These changes around a salinity of 20 are possibly due to introduction of a significant amount of new carbon (autotrophic DOM) to the transect. PC-1 loadings plot shows that spatially differing DOM components are similar to previously reported 13C NMR spectra of heteropolysaccharides (HPS) and carboxyl-rich alicyclic molecules (CRAM). Applying two dimensional correlation spectroscopy techniques to 1H NMR spectra from the same samples reveals increases in the contribution of N-acetyl amino sugars, 6-deoxy sugars, and sulfated polysaccharides to HPS components along the salinity transect, which suggests a transition from plant derived carbohydrates to marine produced carbohydrates within the HMW-DOM pool. In contrast to what has been suggested previously, our combined results from 13C NMR, 1H NMR, and FTIR indicate that CRAM consists of at least two different classes of compounds (aliphatic polycarboxyl compounds and lignin-like compounds).

  11. Rapid Robot Design Validation Project

    Data.gov (United States)

    National Aeronautics and Space Administration — Energid Technologies will create a comprehensive software infrastructure for rapid validation of robotic designs. The software will support push-button validation...

  12. Rapid Robot Design Validation Project

    Data.gov (United States)

    National Aeronautics and Space Administration — Energid Technologies will create a comprehensive software infrastructure for rapid validation of robot designs. The software will support push-button validation...

  13. Effect of Combustion Temperature on Soil and Soil Organic Matter Properties: A Study of Soils from the Western Elevation Transect in Central Sierra Nevada, California

    OpenAIRE

    Araya, Samuel Negusse

    2014-01-01

    Fire is a common ecosystem perturbation that affects many soil physical and chemical properties and soil organic matter (SOM). In my Master's thesis, I investigated the effect of combustion temperatures on the physical and chemical properties of five soils from an elevation transect that spans from 210 to 2865 m.a.s.l. along the Western slope of the Sierra Nevada. All soils were formed on a granitic parent material under either oak woodland, oak/mixed-conifer forest, mixed-conifer forest or s...

  14. Expression of hippocampal corticosteroid receptors, as well as corticotrophin-releasing hormone and vasopressin in the hypothalamic paraventricular nucleus, in fornix transected rats

    Institute of Scientific and Technical Information of China (English)

    Fang Han; Hong Liu; Yanhui Zhang; Yuxiu Shi

    2009-01-01

    BACKGROUND: The hippocampus regulates the hypothalamic-pituitary-adrenal axis through negative feedback. The hypothalamic paraventdcular nucleus receives neuronal input from the hippocampus via the fornix.OBJECTIVE: To explore whether the negative feedback effect of the hippocampus on the hypothalamic-pituitary-adrenal axis is contributed to the inhibitory effect of mineralocorticoid receptor (MR) and glucocorticoid receptor (GR) in the hippocampus on the paraventricular nucleus via the fomix.DESIGN, TIME AND SETTING: Randomized, controlled, animal experiment. The study was performed at the Department of Histology and Embryology, China Medical University between September 2006 and September 2008.MATERIALS: Rabbit anti-rat anti-MR and rabbit anti-rat anti-GR antibodies were purchased from Santa Cruz Biotechnology, USA. Rabbit anti-rat anti-corticotrophin releasing hormone (CRH) and rabbit anti-rat anti-arginine vasopressin antibodies were purchased from Wuhan Boster.METHODS: A total of 90 male, Wistar rats were randomly divided into model and sham-surgery groups (n=45). Fornix transection was performed in the model group, while the sham-surgery group underwent surgery, but no fornix transection.MAIN OUTCOME MEASURES: Immunohistochemistry was used to examine MR and GR expression in the hippocampus, as well as CRH and anti-arginine vasopressin in the paraventricular nucleus. Western blot was used to measure alterations in MR, GR, and CRH protein expression following fomix transection.RESULTS: Compared with the sham-surgery group, there were no obvious changes in MR and GR expression in the hippocampus, or CRH and anti-arginine vasopressin expression in the paraventricular nucleus within 4 days of fornix transection. However, after 7-10 days, significantly decreased MR and GR expression in the hippocampus, and increased CRH and anti-arginine vasopressin expression in the paraventricular nucleus were observed (P < 0.05-0.01).CONCLUSION: Negative feedback from the

  15. Biogeochemical plant–soil interaction: Variable element composition in leaves of four plant species collected along a south–north transect at the southern tip of Norway

    Energy Technology Data Exchange (ETDEWEB)

    Reimann, C., E-mail: clemens.reimann@ngu.no [Geological Survey of Norway (NGU), PO Box 6315 Sluppen, N-7491 Trondheim (Norway); Englmaier, P. [Department of Freshwater Ecology, Faculty of Life Science, University of Vienna, Althanstr. 14, A-1090 Vienna (Austria); Fabian, K. [Geological Survey of Norway (NGU), PO Box 6315 Sluppen, N-7491 Trondheim (Norway); Gough, L. [US Geological Survey National Centre, MS 954, Reston, VA 20192 (United States); Lamothe, P. [US Geological Survey, Denver Federal Centre, Box 25046, MS-964, Denver, CO 80225 (United States); Smith, D. [US Geological Survey, Denver Federal Centre, Box 25046, MS-973, Denver, CO 80225 (United States)

    2015-02-15

    Leaves from four different plant species (birch, willow, juniper, and heather) together with samples of the soil O and C horizons were collected at 44–46 sites along a south-to-north transect extending inland for 200 km from the southern tip of Norway. The transect covers one of the steepest vegetation gradients on Earth, crossing six vegetation zones. Juniper and heather are evergreen, and preferably exclude potentially toxic elements to avoid their accumulation in assimilating tissues, birch and willow shed their leaves in autumn together with the load of potentially toxic elements, and thus can tolerate the uptake of such elements. The plant leaves show the highest concentrations for B, Ca, K, Mg, Mn, P, Rb and S. In the soil O-horizon Ag, Au, As, Bi, Cu, Ge, Hg, In, Pb, Sb, Se, Sn, Te and W are enriched with respect to the C-horizon, whilst Mn and Rb are depleted. Cadmium, Sr and Zn are enriched in willow and Cs, Na and Tl in heather. In terms of concentration gradients from the coast inland, two different patterns are detected: 1) short range with an almost exponential decrease of concentrations from the coast, which appears to be typical for seaspray-related element input, and 2) long range with an almost linear decrease of concentrations with distance from the coast. These patterns differ among the four species, even for one and the same element. Inter-element correlation is different from material to material. Along the transect each of the different plants at the same site individually adapts to the available element combination. High linear correlations in the plants occur between the lanthanides (La, Ce, Y), and interestingly, between P and Ti. The plant/soil system appears highly non-linear and self-regulated. - Highlights: • Plant–soil element uptake and interactions were studied along a 200 km transect. • 53 elements were analysed in leaves of 4 plants and 2 soil horizons. • Element relations within and between the materials revealed

  16. Etude géologique de la Serrania del Interior Oriental (Venezuela sur le transect Cariaco-Maturin Geological Survey of the Serrania Del Interior Oriental in Venezuela on the Cariaco-Maturin Transect

    Directory of Open Access Journals (Sweden)

    Hernandez G.

    2006-11-01

    Full Text Available La Serrania del Interior Oriental, située au nord-est du Venezuela, constitue l'avant-pays plissé de la chaîne Caraïbes qui appartient à la zone frontière entre plaques Caraïbes et Amérique du Sud. Elle est limitée au Nord par le décrochement dextre est-ouest de El Pilar et s'ennoie au Sud sous les séries post-tectoniques du bassin de Maturin. L'étude stratigraphique et structurale, réalisée sur un transect nord-sud normal aux structures, s'appuie sur des données de terrain et de sub-surface; elle a permis de définir deux grandes périodes : - Du Néocomien(?-Barrémien au Miocène inférieur basal, le secteur correspond à une vaste plate-forme continentale, marquée par la concurrence des faciès marins au Nord et des sédiments terrigènes issus du craton guyanais au Sud. La sédimentation enregistre les variations eustatiques (en particulier la grande transgression du Crétace supérieur et la régression de l'Eocène superieur-Oligocène ainsi que des mouvements épirogéniques (Aptien supérieur, Sénonien supérieur, Eocène inférieur et moyen, mais aucun bouleversement paléogéographique. - A partir du Miocène inférieur terminal, le soulèvement de la bordure septentrionale de la région (début de tectonisation de la Serrania del Interior Oriental limite la sédimentation marine à un sillon méridional très subsident (foreland basin : le futur bassin de Maturin. Jusqu'au Miocène moyen terminal, son flanc nord sera progressivement incorporé à l'édifice structural au cours de trois pulsations tectoniques rapprochées; parallèlement, seront repoussés vers le Sud les dépocentres successifs du bassin toujours très subsident. Ce dernier sera ensuite comblé (et repoussé vers l'Est par des sédiments marins peu profonds puis continentaux. L'organisation structurale de la Serrania est caractérisée par un système de plis de direction N 70, souvent coffrés, disposés en échelon par rapport à la faille

  17. Novel Bloodless Potassium Determination Using a Signal‐Processed Single‐Lead ECG

    OpenAIRE

    Attia, Zachi I.; DeSimone, Christopher V.; Dillon, John J.; Sapir, Yehu; Somers, Virend K.; Dugan, Jennifer L.; Bruce, Charles J; Ackerman, Michael J.; Asirvatham, Samuel J.; Striemer, Bryan L.; Bukartyk, Jan; Scott, Christopher G; Bennet, Kevin E.; Ladewig, Dorothy J.; Gilles, Emily J.

    2016-01-01

    Background Hyper‐ and hypokalemia are clinically silent, common in patients with renal or cardiac disease, and are life threatening. A noninvasive, unobtrusive, blood‐free method for tracking potassium would be an important clinical advance. Methods and Results Two groups of hemodialysis patients (development group, n=26; validation group, n=19) underwent high‐resolution digital ECG recordings and had 2 to 3 blood tests during dialysis. Using advanced signal processing, we developed a persona...

  18. Dimethyl Ether Sector Develops Rapidly

    Institute of Scientific and Technical Information of China (English)

    Zong Yangong

    2007-01-01

    @@ Rapid capacity expansion Dimethyl ether production in China has developed rapidly in recent years. The total capacity of dimethyl ether in China was only 31.8 thousand t/a in 2002, the output was around 20 thousand tons and the operating rate was 63%. The number of producers increased to over 30 in 2006.

  19. Effects of C8 ventral root avulsion or transection on spinal alpha motoneurons in adult rats A qualitative light and electron microscopic study

    Institute of Scientific and Technical Information of China (English)

    Khulood M.AL-Khater; Bassem Y.Sheikh

    2008-01-01

    BACKGROUND:Nerve root avulsion is a frequent finding in patients with brachial plexus injury following road traffic accidents or as a result of severe arm traction during complicated deliveries.This injury constitutes a challenging clinical and surgical problem.The orphological characteristics of motoneurons after nerve root avulsion deserve further analysis.OBJECTIVE:To study the different morphological changes of u -motoneurons under light and electron microscopy after C8 spinal ventral rootlets avulsion and transection at various stages.DESIGN:Controlled animal study.SETTING:Department of Anatomy,King Faisal University.MATERIALS:The experiment was carried out at the Department of Anatomy,College of Medicine,King Faisal University between January 2005 and March 2006.Six adult Sprague Dawley rats weighing 200-350 g, irrespective of gender,were used for this study.The animals were bred at the animal house,College of Medicine,King Faisal University,and fed on rat maintenance diet.Water and standard diet were supplied ad libitum.Animal interventions were carried out according to animal ethical standards.METHODS:Three animals were randomly chosen for avulsion of the right ventral rootlets of C8 spinal nerves.The other three received transection of the right ventral rootlets of C8 spinal nerves.①Avulsion experiment:After rats were anesthetized,the right ventral rootlets of C8 spinal nerves were identified.The ventral rootlets were avulsed from the spinal cord by traction with a fine hook(Fine Science Tools Inc.,No. 10031-13,Germany).Traction was exerted in a direction parallel to the course of the spinal root.Under the operating microscope,the Cs segment was exactly located.After checking the successfulness of the surgical procedure,the Ca segment was separated from the spinal cord.The outcome of the avulsion procedure was as follows:two animals had true avulsion,i.e.,no remaining stump was attached to the spinal cord surface.One rat had a stump still attached

  20. Abundance, distribution and size structure of zooplankton along a 20° west meridional transect of the northeast Atlantic Ocean in July

    Science.gov (United States)

    Gallienne, C. P.; Robins, D. B.; Woodd-Walker, R. S.

    Zooplankton distribution, abundance and size structure were characterised on leg 2 of the PRIME cruise in the northeast Atlantic during July 1996. Latitudinal sampling was carried out from 60°N to 37°N along a meridian at approximately 20°W. Zooplankton were characterised using an Optical Plankton Counter (OPC), and from traditional depth-integrated net hauls for taxonomy and carbon. The former method allows zooplankton to be described in terms of biovolume and size. There was good agreement between the two methods for abundance and a good correlation between OPC biovolume and carbon analysis. These data also showed compatibility with concurrent acoustic measurements of zooplankton. Results for the cruise compared to recent studies in the region, show that, overall abundance was generally low, although most of the previous studies were closer to spring bloom conditions. The size structure over latitude shows that mean size fluctuated, but there was a general trend of decreasing mean size from the north of the transect to about 47°N. Further statistical treatment of the OPC data, using principal component analysis, showed that zooplankton size structure changed at five positions along the transect. Three of these coincided with changes in physical structure, one each side of the front at 48°N and the third on the outer edge of an eddy. The other two occurred in regions of high-surface chlorophyll concentration.

  1. Spatial-temporal dynamics of chemical composition of surface snow in East Antarctic along the transect Station Progress-Station Vostok

    Science.gov (United States)

    Khodzher, T. V.; Golobokova, L. P.; Shibaev, Y. A.; Lipenkov, V. Y.; Petit, J. R.

    2013-05-01

    This paper presents data on chemical composition of the Antarctic snow sampled during the 53rd Russian Antarctic Expedition (RAE, 2008) along the first tractor traverse (TT) from Station Progress to Station Vostok (East Antarctica). Snow samples were obtained from the cores drilled at 55.3, 253, 337, 369, 403, 441, 480, 519, 560, 618, 819, and 1276 km from Station Progress. Data on horizontal and deep distribution of chemical components in the snow provide evidence of spatial and temporal variations of conditions for the snow cover formation along the transect under study. Sea salt was the main source for chemical composition of snow cover near the ice edge. Concentrations of marine-derived components decreased further inland. A hypothesis was put forward that some ions in the snow cover of the central part of East Antarctica were likely to be of continental origin. Elevated concentrations of sulphate ions of continental origin were recorded in some profiles of the transect at a depth of 130-150 cm which was attributed to buried signals of the Pinatubo volcano eruption (1991).

  2. A Radio-telemetric System to Monitor Cardiovascular Function in Rats with Spinal Cord Transection and Embryonic Neural Stem Cell Grafts

    Science.gov (United States)

    Hou, Shaoping; Blesch, Armin; Lu, Paul

    2014-01-01

    High thoracic or cervical spinal cord injury (SCI) can lead to cardiovascular dysfunction. To monitor cardiovascular parameters, we implanted a catheter connected to a radio transmitter into the femoral artery of rats that underwent a T4 spinal cord transection with or without grafting of embryonic brainstem-derived neural stem cells expressing green fluorescent protein. Compared to other methods such as cannula insertion or tail-cuff, telemetry is advantageous to continuously monitor blood pressure and heart rate in freely moving animals. It is also capable of long term multiple data acquisitions. In spinal cord injured rats, basal cardiovascular data under unrestrained condition and autonomic dysreflexia in response to colorectal distension were successfully recorded. In addition, cardiovascular parameters before and after SCI can be compared in the same rat if a transmitter is implanted before a spinal cord transection. One limitation of the described telemetry procedure is that implantation in the femoral artery may influence the blood supply to the ipsilateral hindlimb. PMID:25350486

  3. Variability of the sorption of Cs, Zn, Sr, Co, Cd, Ce, Ru, Tc and I at trace concentrations by a forest soil along a transect

    International Nuclear Information System (INIS)

    The variability of the sorption properties (characterized by the distribution coefficient Kd) of a forest soil for Cs, Zn, Sr, Co, Cd, Ce, Ru, Tc, and I were determined along a transect of 150 m in regular intervals of 3 m. Radioactive tracers were used to ensure trace concentrations of these elements. For comparison, for each soil sample the loss on ignition, as a measure of the soil organic matter content, and the pH were also determined. On average, the Kd values increase in the sequence Tc d values for some elements changed in a similar, and for others in a dissimilar pattern. In a few case a correlation between the pH or the loss on ignition and the Kd values was also present. The spatial structure of the data was examined by semivariograms. For the elements Co, Cd, Zn, and Sr, the Kd values showed an almost periodic behavior along the transect, which is probably due to periodic changes of some soil properties in a forest with regularly spaced trees

  4. Comparisons of surface Chl a and primary productivity along three transects of the southern South China Sea, northern Java Sea and eastern Indian Ocean in April 2011

    Institute of Scientific and Technical Information of China (English)

    YI Rong; KE Zhi-xin; SONG Xing-yu; SHEN Ping-ping; WANG Sheng-fu; FAN Yan-zhi; HUANG Liang-min; TAN Ye-hui; LI Gang

    2014-01-01

    Results are presented about the changes in chlorophyll a density, carbon fixation and nutrient levels in the surface waters of three transects of the southern South China Sea (SCS), northern Java Sea (JS) and eastern Indian Ocean (IO) during April 5~16 of 2011. The in situ Chl a concentration and carbon fixation showed decreasing trends from high to low latitude along the three transects, while the photosynthetic rate of phytoplankton estimated from 14C incorporation displayed no simple variation with latitude. Chl a concentration and carbon fixation in the IO water was lower than that in the JS water. Higher salinity and lower contents of dissolved inorganic nitrogen (DIN) and silicate (SiO32−) characterized the IO water as compared to the SCS or JS water, and the PO43−content was lower in the IO water than in the SCS or JS water in most cases. Our results also indicate the importance of DIN and SiO32− concentrations for the geographical changes in phytoplankton biomass and primary productivity among the three regions.

  5. Variations in Pb concentrations and Pb-isotope ratios in soils collected along an east-west transect across the United States

    Science.gov (United States)

    Smith, David; Woodruff, Laurel; Reimann, Clemens; Flem, Belinda

    2014-05-01

    Soil A-horizon and C-horizon samples were collected along a 4000 km long transect cutting the USA from the west to the east coast. For purposes of site selection, the transect was divided into approximately 40-km segments. For each segment, a 1-km2 target area was selected at random. Soil A- and C-horizon samples were collected at a site within each target area that was most representative of the surrounding landscape. The samples were air-dried at ambient temperature, disaggregated, and sieved through a 2-mm stainless steel screen. The soil A-horizon and 137 soil C-horizon samples on an inductively coupled sector field plasma mass spectrometer (SF-ICP-MS; ELEMENT 1, Finnigan MAT) in the laboratory of the Geological Survey of Norway (NGU), following a 7 N HNO3 digestion. Lead concentrations along the transect show (1) generally higher values in the soil A-horizon than the C-horizon (median 21 vs. 16.5 mg/kg), (2) an increase in the median value of the soil A-horizon for the central to eastern U.S. (Missouri to Maryland) when compared to the western U.S. (California to Kansas) (median 26 vs. 20 mg/kg) and (3) a higher A/C ratio for the central to eastern US (1.35 vs. 1.14). Lead isotopes show a distinct trend across the U.S., with the highest 206Pb/207Pb ratios occurring in the centre (Missouri, median A-horizon: 1.245; C-horizon: 1.251) and the lowest at both coasts (e.g. California, median A-horizon: 1.195; C-horizon:1.216). The soil C-horizon samples show generally higher 206Pb/207Pb ratios than the A-horizon (median C-horizon: 1.224; A-horizon: 1.219). The 206Pb/207Pb isotope ratios in the soil A horizon show a correlation with the total feldspar content for the same 2500-km portion of the transect from east-central Colorado to the Atlantic coast that shows steadily increasing precipitation. No such correlation exists in the soil C horizon. The data demonstrate the importance of climate and weathering on both Pb concentration and 206Pb/207Pb isotope ratios in

  6. How Rapid is Rapid Prototyping? Analysis of ESPADON Programme Results

    Directory of Open Access Journals (Sweden)

    Ian D. Alston

    2003-05-01

    Full Text Available New methodologies, engineering processes, and support environments are beginning to emerge for embedded signal processing systems. The main objectives are to enable defence industry to field state-of-the-art products in less time and with lower costs, including retrofits and upgrades, based predominately on commercial off the shelf (COTS components and the model-year concept. One of the cornerstones of the new methodologies is the concept of rapid prototyping. This is the ability to rapidly and seamlessly move from functional design to the architectural design to the implementation, through automatic code generation tools, onto real-time COTS test beds. In this paper, we try to quantify the term “rapid” and provide results, the metrics, from two independent benchmarks, a radar and sonar beamforming application subset. The metrics show that the rapid prototyping process may be sixteen times faster than a conventional process.

  7. Rapid Multiplex Microbial Detector Project

    Data.gov (United States)

    National Aeronautics and Space Administration — ORBITEC, in collaboration with Lucigen, proposes a rapid nucleic acid-based detector for spaceflight water systems to enable simultaneous quantification of multiple...

  8. A Rapid Coliform Detector Project

    Data.gov (United States)

    National Aeronautics and Space Administration — ORBITEC, in collaboration with Lucigen, proposes a rapid genetic detector for spaceflight water systems to enable real-time detection of E-coli with minimal...

  9. Rapid deployment intrusion detection system

    International Nuclear Information System (INIS)

    A rapidly deployable security system is one that provides intrusion detection, assessment, communications, and annunciation capabilities; is easy to install and configure; can be rapidly deployed, and is reusable. A rapidly deployable intrusion detection system (RADIDS) has many potential applications within the DOE Complex: back-up protection for failed zones in a perimeter intrusion detection and assessment system, intrusion detection and assessment capabilities in temporary locations, protection of assets during Complex reconfiguration, and protection in hazardous locations, protection of assets during Complex reconfiguration, and protection in hazardous locations. Many DOE user-need documents have indicated an interest in a rapidly deployable intrusion detection system. The purpose of the RADIDS project is to design, develop, and implement such a system. 2 figs

  10. Economical Investigation of Rapid Prototyping

    OpenAIRE

    Péter Ficzere; Lajos Borbás; Ádám Török

    2013-01-01

    Usage of rapid prototyping gain developers, designers and engineers more time and consume less money and save more resources. In this article authors investigated the economic possibilities of rapid prototyping. In concurrent engineering, different tasks are tackled at the same time, and not necessarily in the usual order. Concurrent engineering is a method by which several teams within an organization work simultaneously to develop new products and allows more flexible approach. The concurre...

  11. Dilepton distributions at backward rapidities

    CERN Document Server

    Betemps, M A; De Oliveira, E G

    2006-01-01

    The dilepton production at backward rapidities in $pAu$ and $pp$ collisions at RHIC and LHC energies is investigated in the dipole approach. The results are shown through the nuclear modification ratio $R_{pA}$ considering the transverse momentum and rapidity spectra. The dilepton modification ratio presents interesting behavior at the backward rapidities once compared with the already known forward one, since it is related with large $x$ kinematical region that is being probed. The nuclear modification ratio rapidity spectrum in the dilepton production is strongly dependent on the Bjorken $x$ behavior of the nuclear structure function ratio $R_{F_{2}}=F_{2}^{A}/F_{2}^{p}$. The $R_{pA}$ transverse momentum dependence at backward rapidities is modified due to the large $x$ nuclear effects: at RHIC energies, for instance, the ratio $R_{pA}$ is reduced as $p_T$ increases, presenting oppositive behavior once compared with the forward one. It implies the dilepton production at backward rapidities should carry info...

  12. Beyond the transect: An alternative microchemical imaging method for fine scale analysis of trace elements in fish otoliths during early life

    International Nuclear Information System (INIS)

    Microchemical analysis of otolith (calcified ‘ear stones’ used for balance and orientation) of fishes is an important tool for studying their environmental history and management. However, the spatial resolution achieved is often too coarse to examine short-term events occurring in early life. Current methods rely on single points or transects across the otolith surface, which may provide a limited view of elemental distributions, a matter that has not previously been investigated. Imaging by laser ablation-inductively coupled plasma-mass spectrometry (LA-ICP-MS) permits microchemical analyses of short-term events in early life with high (< 10 μm) resolution, two-dimensional (2D) visualization of elemental distributions. To demonstrate the potential of this method, we mapped the concentrations of Sr and Ba, two key trace elements, in a small number of juvenile otoliths of neon damselfish (Pomacentrus coelestis) using an 8 μm beam diameter (laser fluence of 13.8 ± 3.5 J cm−2). Quantification was performed using the established method by Longerich et al. (1996), which is applied to 2D imaging of a biological matrix here for the first time. Accuracy of > 97% was achieved using a multi-point non matrix-matched calibration of National Institute of Standards and Technology (NIST) 610 and 612 (trace elements in glass) using Longerich's calculation method against the matrix-matched standard FEBS-1 (powdered red snapper [Lutjanus campechanus] otolith). The spatial resolution achieved in the otolith corresponded to a time period of 2 ± 1 days during the larval phase, and 4 ± 1 days during the post-settlement juvenile phase. This method has the potential to improve interpretations of early life-history events at scales corresponding to specific events. While the images showed gradients in Sr and Ba across the larval settlement zone more clearly than single transects, the method proved sample homogeneity throughout the structure; demonstrating that 2D scanning

  13. Beyond the transect: An alternative microchemical imaging method for fine scale analysis of trace elements in fish otoliths during early life

    Energy Technology Data Exchange (ETDEWEB)

    McGowan, Nicole [Elemental Bio-imaging Facility, University of Technology, Sydney, New South Wales (Australia); CCFS ARC Centre of Excellence, Department of Earth and Planetary Sciences, Macquarie University, Sydney (Australia); Fowler, Ashley M.; Parkinson, Kerryn [Fish Ecology Laboratory, School of the Environment, University of Technology, Sydney, New South Wales (Australia); Bishop, David P. [Elemental Bio-imaging Facility, University of Technology, Sydney, New South Wales (Australia); Ganio, Katherine [Florey Department of Neuroscience and Mental Health, University of Melbourne, Victoria (Australia); Doble, Philip A. [Elemental Bio-imaging Facility, University of Technology, Sydney, New South Wales (Australia); Booth, David J. [Fish Ecology Laboratory, School of the Environment, University of Technology, Sydney, New South Wales (Australia); Hare, Dominic J., E-mail: dominic.hare@uts.edu.au [Elemental Bio-imaging Facility, University of Technology, Sydney, New South Wales (Australia); Florey Department of Neuroscience and Mental Health, University of Melbourne, Victoria (Australia)

    2014-10-01

    Microchemical analysis of otolith (calcified ‘ear stones’ used for balance and orientation) of fishes is an important tool for studying their environmental history and management. However, the spatial resolution achieved is often too coarse to examine short-term events occurring in early life. Current methods rely on single points or transects across the otolith surface, which may provide a limited view of elemental distributions, a matter that has not previously been investigated. Imaging by laser ablation-inductively coupled plasma-mass spectrometry (LA-ICP-MS) permits microchemical analyses of short-term events in early life with high (< 10 μm) resolution, two-dimensional (2D) visualization of elemental distributions. To demonstrate the potential of this method, we mapped the concentrations of Sr and Ba, two key trace elements, in a small number of juvenile otoliths of neon damselfish (Pomacentrus coelestis) using an 8 μm beam diameter (laser fluence of 13.8 ± 3.5 J cm{sup −2}). Quantification was performed using the established method by Longerich et al. (1996), which is applied to 2D imaging of a biological matrix here for the first time. Accuracy of > 97% was achieved using a multi-point non matrix-matched calibration of National Institute of Standards and Technology (NIST) 610 and 612 (trace elements in glass) using Longerich's calculation method against the matrix-matched standard FEBS-1 (powdered red snapper [Lutjanus campechanus] otolith). The spatial resolution achieved in the otolith corresponded to a time period of 2 ± 1 days during the larval phase, and 4 ± 1 days during the post-settlement juvenile phase. This method has the potential to improve interpretations of early life-history events at scales corresponding to specific events. While the images showed gradients in Sr and Ba across the larval settlement zone more clearly than single transects, the method proved sample homogeneity throughout the structure; demonstrating that 2D

  14. A transect across Australia's southern margin in the Otway Basin region: crustal architecture and the nature of rifting from wide-angle seismic profiling

    Science.gov (United States)

    Finlayson, D. M.; Collins, C. D. N.; Lukaszyk, I.; Chudyk, E. C.

    1998-03-01

    The Otway Basin in southeastern Australia formed on a triangular-shaped area of extended continental lithosphere during two extensional episodes in Cretaceous-to-Miocene times which ultimately led to the separation of Australia and Antarctica. The velocity structure and crustal architecture of the Otway continental margin has been interpreted from offshore-onshore wide-angle seismic profiling data along a transect extending from near the northern Otway Basin margin with Palaeozoic outcrop to the deep ocean basin under the Southern Ocean. Along this transect, the Otway Continental Margin (OCM) Transect, the onshore half-graben geometry of Early Cretaceous deposition gives way to a 5-km-thick basin sequence (P-wave velocity 2.2-4.6 km/s) extending down the continental slope offshore to at least 60 km from the shoreline. At 120 km from the nearest shore, sonobuoy data indicate a 4-5 km sedimentary sequence overlying 7 km of crustal basement rocks above the Moho at 15 km depth (water depth 4220 m). Conspicuous strong Moho reflections are evident under the continental slope at about 10.2 s TWT. Basement is interpreted to be attenuated/faulted Palaeozoic rocks of the Delamerian and Lachlan Orogens (intruded with Jurassic volcanics) that thin from 16 km onshore to about 3.5 km at 120 km from the nearest shore. These rocks comprise a 3 km section that has a velocity of 5.5-5.7 km/s overlying deeper basement with a velocity of 6.15-6.35 km/s. Over the same distance the Moho shallows from a depth of 30 km onshore to 15 km depth at 120 km from the nearest shore, and then to about 12 km in the deep ocean at the limits of the profile (water depth 5200 m). The continent-ocean boundary (COB) is interpreted to be at a prominent topographic inflection point at the bottom of the continental slope in 4800 m of water. P-wave velocities in the lower crust are 6.4-6.8 km/s above a transition to the Moho, with an upper mantle velocity of 8.05 km/s. There is no evidence of massive high

  15. Variations in carbon isotope ratios of C_3 plants and distribution of C_4 plants along an altitudinal transect on the eastern slope of Mount Gongga

    Institute of Scientific and Technical Information of China (English)

    LI JiaZhu; WANG GuoAn; LIU XianZhao; HAN JiaMao; LIU Min; LIU XiaoJuan

    2009-01-01

    Variations in carbon isotopic ratios (δ~(13)C) of C_3 plants and distribution of C_4 plants were investigated along an altitudinal transect on the eastern slope of Mount Gongga,and the environmental effects on them were discussed,it is shown that plants with C_4 photosynthetic pathway mainly occur at altitudes below 2100 m a.a.l.,suggesting that the low summer temperature is responsible for the distributional pattern.In addition,δ~(13)C of C_3 plants increases with elevation at the region above 2000 m a.s.l,with the characteristics of humid climate,and the increase rate in δ~(13)C for C_3 plants is about 1.3‰ per kilometer.Temperature determines the altitudinal trend of δ~(13)C.

  16. Dust pollen distribution on a continental scale and its relation to present-day vegetation along north-south transects in east China

    Institute of Scientific and Technical Information of China (English)

    ZHENG Zhuo; P. COUR; HUANG CiXuan; D. DUZER; G. ROBERT; M. CALLEJA; C. BEAUDOUIN; DENG Yun; HUANG KangYou

    2007-01-01

    A series of dust pollen samples was collected along N-S transects in east China (18°N to 53°N latitudes). Sample sites extend from the cold-temperate zone in the north to the tropical region in the south. Pollen taxa characterize each region and reflect the natural and devastated vegetation as well as corresponding climatic zones. The quantitative pollen data can be used to estimate the spatial distribution of planted and introduced species. Valuable information of human disturbance of the natural forest is evaluated by quantitative comparison between dust pollen and in-situ pollen of protected forest. In addition, percentages of grass pollen vary regularly from north to south that is consistent with spatial distribution of net primary productivity in east China. Among all grasses, Artemisia and the Gramineae carry the clearest signal: their ratio increases northwards and therefore represent a suitable and convenient tool for palaeoclimate reconstructions.

  17. Dust pollen distribution on a continental scale and its relation to present-day vegetation along north-south transects in east China

    Institute of Scientific and Technical Information of China (English)

    P.; COUR; D.; DUZER; G.; ROBERT; M.; CALLEJA; C.; BEAUDOUIN

    2007-01-01

    A series of dust pollen samples was collected along N-S transects in east China (18°N to 53°N latitudes). Sample sites extend from the cold-temperate zone in the north to the tropical region in the south. Pollen taxa characterize each region and reflect the natural and devastated vegetation as well as corre- sponding climatic zones. The quantitative pollen data can be used to estimate the spatial distribution of planted and introduced species. Valuable information of human disturbance of the natural forest is evaluated by quantitative comparison between dust pollen and in-situ pollen of protected forest. In addition, percentages of grass pollen vary regularly from north to south that is consistent with spatial distribution of net primary productivity in east China. Among all grasses, Artemisia and the Gramineae carry the clearest signal: their ratio increases northwards and therefore represent a suitable and con- venient tool for palaeoclimate reconstructions.

  18. Variations in carbon isotope ratios of C3 plants and distribution of C4 plants along an altitudinal transect on the eastern slope of Mount Gongga

    Institute of Scientific and Technical Information of China (English)

    2009-01-01

    Variations in carbon isotopic ratios (δ 13C) of C3 plants and distribution of C4 plants were investigated along an altitudinal transect on the eastern slope of Mount Gongga, and the environmental effects on them were discussed. It is shown that plants with C4 photosynthetic pathway mainly occur at altitudes below 2100 m a.s.l., suggesting that the low summer temperature is responsible for the distributional pattern. In addition, δ 13C of C3 plants increases with elevation at the region above 2000 m a.s.l. with the characteristics of humid climate, and the increase rate in δ 13C for C3 plants is about 1.3‰ per kilometer. Temperature determines the altitudinal trend of δ 13C.

  19. A 26-year clinical observation of splenic auto-transplantation and oesophageal transection anastomosis: a new treatment strategy in patients with portal hypertension

    Institute of Scientific and Technical Information of China (English)

    ZHANG Lei; CHEN Ji-sheng; HUO Jin-shan; ZHANG Hong-wei; CHEN Ru-fu; ZHANG Jie; Obetien Mapudengo; FANG Tian-lin; CHEN Ya-jin; OU Qing-jia

    2007-01-01

    Background Surgical treatment options for patients with cirrhosis and portal hypertension are complicated. In this study,we evaluated the effectiveness of a new treatment strategy, splenic auto-transplantation and oesophageal transection anastomosis. We report results from clinical observations, splenic immune function and portal dynamics in 274 patients.Methods From 1979 to 2005, 274 cirrhosis patients with portal hypertension underwent the new treatment strategy, and were followed up to compare results with those patients who underwent traditional surgical treatment. From 1999 to 2002,a randomized controlled trial (RCT) was performed on 40 patients to compare their post-operative immune function. From 1994 to 2006, another RCT enrolled 28 patients to compare portal dynamics using three-dimensional dynamic contrast-enhanced magnetic resonance angiography (3D DEC MRA) investigation post operation.Results Among 274 patients (mean age 41.8 years), the emergency operative mortality (4.4%), selective operative mortality (2.2%), complication rate (17.9%), prevalence of hepatic encephalopathy (<1%), rate of portal hypertension gastritis (PHG) bleeding (9.1%), and morbidity of hepatic carcinoma (8%) were similar to those patients undergoing traditional operation; the spleen immunology function (Tuftsin, IgM) decreased in both groups 2 months post operation,but this decrease did not reach statistical significance. Through 3D DCE MRA, the cross sectional area and the velocity and volume of blood flow of the main portal vein decreased significantly after operation in both groups. The velocity and volume of blood flow in the auto-transplantation group was significantly lower than that in the control group.Conclusions Splenic auto-transplantation and esophageal transection anastomosis is a safe, effective, and reasonable treatment strategy for patients with portal hypertension with varicial bleeding. It not only can correct hypersplenism, but may also achieve complete

  20. Evolution of Temperature and Carbon Storage Within the Deep Southeast Atlantic Ocean Across the Last Glacial/Interglacial Cycle Inferred from a Highly-Resolved Sedimentary Depth Transect

    Science.gov (United States)

    Foreman, A. D.; Charles, C. D.; Rae, J. W. B.; Adkins, J. F.; Slowey, N. C.

    2015-12-01

    Many models show that the relative intensity of stratification is a primary variable governing the sequestration and release of carbon from the ocean over ice ages. The wide-scale observations necessary to test these model-derived hypotheses are not yet sufficient, but sedimentary depth transects represent a promising approach for making progress. Here we present paired stable isotopic (d18O, d13C) and trace metal data (Mg/Ca, B/Ca) from benthic foraminifera collected from a highly vertically-resolved depth transect from the mid-depth and deep SE Atlantic. These observations, which cover Marine Isotope Stages 5e, 5d, 5a, 4, and the Last Glacial Maximum, document the evolution of glacial conditions from the previous interglacial, and provide detailed observations regarding the magnitude and timing of changes in temperature and salinity within the deep ocean at key time points over the last glacial/interglacial cycle. Furthermore, the comparison between purely 'physical' tracers (i.e. Mg/Ca, d18O) and tracers sensitive to the carbon cycle (i.e. d13C and B/Ca) provides critical insight into the relationship between deep/mid-depth stratification and global carbon dynamics. Notably among our observations, the paired stable isotope and trace metal results strongly suggest that much of the ice-age cooling of deep South Atlantic occurred at the MIS 5e/5d transition, while the onset of salinity stratification in the mid-depth South Atlantic occurred at the MIS 5/4 transition.

  1. Iron oxide nanoparticles and magnetic field exposure promote functional recovery by attenuating free radical-induced damage in rats with spinal cord transection

    Directory of Open Access Journals (Sweden)

    Pal A

    2013-06-01

    Full Text Available Ajay Pal,1 Anand Singh,2 Tapas C Nag,3 Parthaprasad Chattopadhyay,2 Rashmi Mathur,1 Suman Jain1 1Department of Physiology, 2Department of Biochemistry, 3Department of Anatomy, All India Institute of Medical Sciences, New Delhi, India Background: Iron oxide nanoparticles (IONPs can attenuate oxidative stress in a neutral pH environment in vitro. In combination with an external electromagnetic field, they can also facilitate axon regeneration. The present study demonstrates the in vivo potential of IONPs to recover functional deficits in rats with complete spinal cord injury. Methods: The spinal cord was completely transected at the T11 vertebra in male albino Wistar rats. Iron oxide nanoparticle solution (25 µg/mL embedded in 3% agarose gel was implanted at the site of transection, which was subsequently exposed to an electromagnetic field (50 Hz, 17.96 µT for two hours daily for five weeks. Results: Locomotor and sensorimotor assessment as well as histological analysis demonstrated significant functional recovery and a reduction in lesion volume in rats with IONP implantation and exposure to an electromagnetic field. No collagenous scar was observed and IONPs were localized intracellularly in the immediate vicinity of the lesion. Further, in vitro experiments to explore the cytotoxic effects of IONPs showed no effect on cell survival. However, a significant decrease in H2O2-mediated oxidative stress was evident in the medium containing IONPs, indicating their free radical scavenging properties. Conclusion: These novel findings indicate a therapeutic role for IONPs in spinal cord injury and other neurodegenerative disorders mediated by reactive oxygen species. Keywords: secondary damage, oxidative stress, electromagnetic field, cytotoxicity, neurodegeneration, pain

  2. Rapid Manufacturing - auch mit SLM

    OpenAIRE

    Spierings, Adriaan; Levy, Gideon

    2009-01-01

    Rapid Manufacturing (RM) wurde als Schlagwort in der letzten Zeit insbesondere aus dem Bereich des Selective Laser Sintering (SLS) bekannt. In dieser inzwischen über 15-jährigen Technologieentwicklung wurden in den vergangenen Jahren bedeutende Fortschritte erzielt, die die Bauteileigenschaften nahe an die Anforderungen für End-Teile heran brachten. So ist das RM denn auch weniger aus der Sicht grösserer Losgrösse zu verstehen. Viel mehr bedeutet Rapid Manufacturing, dass die Bauteile nach ei...

  3. Knowledge Exchange Within the Rapid Climate Change Program (RAPID)

    Science.gov (United States)

    Wallace, C.; Srokosz, M.

    2008-12-01

    The Rapid Climate Change Program (RAPID) is a major UK-led science program investigating the susceptibility to and risk of abrupt change in the climate system, with a particular focus on the role of the Atlantic Meridional Overturning Circulation (AMOC). RAPID commenced in 2001, with initial funding to 2008 and has just entered its second phase - RAPID-WATCH - extending some aspects of the program until 2014. Scientifically, a key focus of the program has concerned the development and deployment of a continuous AMOC observing system, although the program has also supported research into a number of other aspects of rapid climate change, including paleo studies of past rapid changes, modeling of the past and future climate, and the development of methods with which to aid the quantification of future risks. Given the societal implications that abrupt climate change associated with the AMOC may have for the North Atlantic region, a major focus within the program has been the implementation of a Knowledge Exchange strategy with which to engage key decision and policy makers within the UK political and industrial sectors. This interaction is especially pertinent for those decision makers involved in the development of adaptive capacities that must incorporate resilience to so-called high-impact, low-probability class threats. We will summarize here the main aspects of our approach, outlining activities/methods used both to satisfy the obvious need to transfer key scientific findings from the program to users, but also in facilitating a return flow of knowledge which can be used by scientists to refine the impact and utility of scientific output. We will draw on particular experience gained from the program's involvement in i: personnel exchanges with central government departments; ii: interaction and co-operation with third party stakeholder organizations (e.g. the UK Climate Impacts Program); and iii: provision of written and oral briefings. Throughout, techniques

  4. Rapid Prototyping Enters Mainstream Manufacturing.

    Science.gov (United States)

    Winek, Gary

    1996-01-01

    Explains rapid prototyping, a process that uses computer-assisted design files to create a three-dimensional object automatically, speeding the industrial design process. Five commercially available systems and two emerging types--the 3-D printing process and repetitive masking and depositing--are described. (SK)

  5. Rapidly Deployed Modular Telemetry System

    Science.gov (United States)

    Varnavas, Kosta A. (Inventor); Sims, William Herbert, III (Inventor)

    2013-01-01

    The present invention is a telemetry system, and more specifically is a rapidly deployed modular telemetry apparatus which utilizes of SDR technology and the FPGA programming capability to reduce the number of hardware components and programming required to deploy a telemetry system.

  6. Rapid thermal processing of semiconductors

    CERN Document Server

    Borisenko, Victor E

    1997-01-01

    Rapid thermal processing has contributed to the development of single wafer cluster processing tools and other innovations in integrated circuit manufacturing environments Borisenko and Hesketh review theoretical and experimental progress in the field, discussing a wide range of materials, processes, and conditions They thoroughly cover the work of international investigators in the field

  7. Evolution of rapid nerve conduction.

    Science.gov (United States)

    Castelfranco, Ann M; Hartline, Daniel K

    2016-06-15

    Rapid conduction of nerve impulses is a priority for organisms needing to react quickly to events in their environment. While myelin may be viewed as the crowning innovation bringing about rapid conduction, the evolution of rapid communication mechanisms, including those refined and enhanced in the evolution of myelin, has much deeper roots. In this review, a sequence is traced starting with diffusional communication, followed by transport-facilitated communication, the rise of electrical signaling modalities, the invention of voltage-gated channels and "all-or-none" impulses, the emergence of elongate nerve axons specialized for communication and their fine-tuning to enhance impulse conduction speeds. Finally within the evolution of myelin itself, several innovations have arisen and have been interactively refined for speed enhancement, including the addition and sealing of layers, their limitation by space availability, and the optimization of key parameters: channel density, lengths of exposed nodes and lengths of internodes. We finish by suggesting several design principles that appear to govern the evolution of rapid conduction. This article is part of a Special Issue entitled SI: Myelin Evolution. PMID:26879248

  8. Fisheries Biology and Stock Assessment Division (FBSAD) Recruit and Predator Reef Fish Belt Transect and Habitat Quadrat Surveys at Hawaii Island and Midway Atoll, 2006-2007; and Oahu, 2007 only (NODC Accession 0056602)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Shore-based belt and habitat quadrats were surveyed using transects at 1 to ~5 m depths at a total seven (7) sites: at (1-2) 2 longshore sites on the leeward coast...

  9. FBSAD RECRUIT Reef Fish Belt Transect Survey at Hawaii Island (Big Island), Main Hawaiian Islands, 2006 and 2007; Oahu (Kaneohe Bay), Main Hawaiian Islands, 2007 only; and Midway Atoll, Northwestern Hawaiian Islands (NWHI), 2006 and 2007 (NODC Accession 0056602)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Shore-based belt transects were conducted at 1 to ~ 5 m depths at a total seven (7) sites: at (1-2) 2 longshore sites on the leeward coast (South Kohala district)...

  10. FBSAD PREDATOR Reef Fish Belt Transect Survey at Hawaii Island (Big Island), Main Hawaiian Islands, 2006 and 2007; Oahu (Kaneohe Bay), Main Hawaiian Islands, 2007 only; and Midway Atoll, Northwestern Hawaiian Islands (NWHI), 2006 and 2007 (NODC Accession 0056602)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Shore-based belt transects were conducted at 1 to ~5 m depths at a total seven (7) sites: at (1-2) 2 longshore sites on the leeward coast (South Kohala district) of...

  11. Characteristics of soil grain size in the southern and northern transections of Ebinur Lake region%艾比湖地区南北典型断面的土壤粒度特征

    Institute of Scientific and Technical Information of China (English)

    常轶深; 钱亦兵; 王忠臣; 马玉娥; 杨林

    2012-01-01

    通过对艾比湖典型断面土壤粒度特征分析,发现研究区粒径组成以中粉沙为主,质地以砂质壤土为主,土壤垂向粗骨化明显.土壤分选性较差,粒度分布多为正偏,峰态多为窄峰态,表明该区土壤母质来源丰富,搬运—沉积环境异质性显著,但流水作用突出.北断面的分维值高于南断面,这与南断面分选性好于北断面相吻合.不同地区的概率累计曲线类型各异,但都富含悬移组分,并且对环境敏感的粒度组分大都在60 μm上下,说明研究区土壤母质以水成沉积环境为主的基础上,叠加有风成碎屑.%In August 2010, the field investigation was conducted in the southern and northern transections of the Ebinur Lake region, and different vegetation parameters and 39 soil samples were collected according to the natural conditions of geomorphology, vegetation, and soil in this study. The elevation was obtained with GPS positioning for each sample site. The physical-chemical properties of 78 soil samples, which were collected according to the layers of soil genesis, were analyzed in the lab. The composition of grain-sizes of soil samples was measured using Malv-em -2 000 for the range of 0. 02 -1 000 μm and the grain-sizes of larger than 1 000 μm were measured by mechanical screening method. Grain-size parameters were calculated by Folk and Ward formula. The Visher cumulative probability size-frequency curves and environment sensitive grain-size curves also were plotted. Finally, the fractal dimension values of soil samples were analyzed by exponential model. The results show that the soils are mainly composed by medium silt in the study area; the texture is dominated by sandy loam; and the coarseness of the soils in the vertical profiles is significant. The sorting of soils in Ebinur Lake region is poorer. Most of the grain size patterns are positive-skewed. The kurtosis of most soils is a narrow spike state, showing that the sources of soil' s

  12. Rapid and sustained cost management

    International Nuclear Information System (INIS)

    Accenture helps clients develop comprehensive, process-driven strategies for rapid and sustained cost management that leverage deep insights and analytics. This approach enables companies to gain operating cost advantages by rationalizing, simplifying and automating current operating capabilities. It drives structural cost advantages by optimizing business mix, capital structure, organizational structure and geographic presence. This paper discussed how successful companies achieve high performance during times of economic turmoil. It also discussed the value of the winner's strategy in terms of rapid and sustained cost management (RSCM). It discussed how Accenture operates and its leveraged capabilities, improved efficiency, margins and cash flow while maintaining customer service levels. Building structural advantage and the Accenture difference were also discussed. It was concluded that RSCM is one vital way that Accenture can help companies achieve success. 4 figs

  13. Rapid Sampling from Sealed Containers

    International Nuclear Information System (INIS)

    The authors have developed several different types of tools for sampling from sealed containers. These tools allow the user to rapidly drill into a closed container, extract a sample of its contents (gas, liquid, or free-flowing powder), and permanently reseal the point of entry. This is accomplished without exposing the user or the environment to the container contents, even while drilling. The entire process is completed in less than 15 seconds for a 55 gallon drum. Almost any kind of container can be sampled (regardless of the materials) with wall thicknesses up to 1.3 cm and internal pressures up to 8 atm. Samples can be taken from the top, sides, or bottom of a container. The sampling tools are inexpensive, small, and easy to use. They work with any battery-powered hand drill. This allows considerable safety, speed, flexibility, and maneuverability. The tools also permit the user to rapidly attach plumbing, a pressure relief valve, alarms, or other instrumentation to a container. Possible applications include drum venting, liquid transfer, container flushing, waste characterization, monitoring, sampling for archival or quality control purposes, emergency sampling by rapid response teams, counter-terrorism, non-proliferation and treaty verification, and use by law enforcement personnel during drug or environmental raids

  14. Exercise-induced motor improvement after complete spinal cord transection and its relation to expression of brain-derived neurotrophic factor and presynaptic markers

    Directory of Open Access Journals (Sweden)

    Sulejczak Dorota

    2009-12-01

    Full Text Available Abstract Background It has been postulated that exercise-induced activation of brain-derived neurotrophic factor (BDNF may account for improvement of stepping ability in animals after complete spinal cord transection. As we have shown previously, treadmill locomotor exercise leads to up-regulation of BDNF protein and mRNA in the entire neuronal network of intact spinal cord. The questions arise: (i how the treadmill locomotor training, supplemented with tail stimulation, affects the expression of molecular correlates of synaptic plasticity in spinal rats, and (ii if a response is related to BDNF protein level and distribution. We investigated the effect of training in rats spinalized at low thoracic segments on the level and distribution of BDNF immunoreactivity (IR in ventral quadrants of the lumbar segments, in conjunction with markers of presynaptic terminals, synaptophysin and synaptic zinc. Results Training improved hindlimb stepping in spinal animals evaluated with modified Basso-Beattie-Bresnahan scale. Grades of spinal trained animals ranged between 5 and 11, whereas those of spinal were between 2 and 4. Functional improvement was associated with changes in presynaptic markers and BDNF distribution. Six weeks after transection, synaptophysin IR was reduced by 18% around the large neurons of lamina IX and training elevated its expression by over 30%. The level of synaptic zinc staining in the ventral horn was unaltered, whereas in ventral funiculi it was decreased by 26% postlesion and tended to normalize after the training. Overall BDNF IR levels in the ventral horn, which were higher by 22% postlesion, were unchanged after the training. However, training modified distribution of BDNF in the processes with its predominance in the longer and thicker ones. It also caused selective up-regulation of BDNF in two classes of cells (soma ranging between 100-400 μm2 and over 1000 μm2 of the ventrolateral and laterodorsal motor nuclei

  15. Oxygen isotope ratios (18O/16O) of hemicellulose-derived sugar biomarkers in plants, soils and sediments as paleoclimate proxy II: Insight from a climate transect study

    Science.gov (United States)

    Tuthorn, Mario; Zech, Michael; Ruppenthal, Marc; Oelmann, Yvonne; Kahmen, Ansgar; Valle, Héctor Francisco del; Wilcke, Wolfgang; Glaser, Bruno

    2014-02-01

    The oxygen isotopic composition of precipitation (δ18Oprec) is well known to be a valuable (paleo-)climate proxy. Paleosols and sediments and hemicelluloses therein have the potential to serve as archives recording the isotopic composition of paleoprecipitation. In a companion paper (Zech et al., 2014) we investigated δ18Ohemicellulose values of plants grown under different climatic conditions in a climate chamber experiment. Here we present results of compound-specific δ18O analyses of arabinose, fucose and xylose extracted from modern topsoils (n = 56) along a large humid-arid climate transect in Argentina in order to answer the question whether hemicellulose biomarkers in soils reflect δ18Oprec. The results from the field replications indicate that the homogeneity of topsoils with regard to δ18Ohemicellulose is very high for most of the 20 sampling sites. Standard deviations for the field replications are 1.5‰, 2.2‰ and 1.7‰, for arabinose, fucose and xylose, respectively. Furthermore, all three hemicellulose biomarkers reveal systematic and similar trends along the climate gradient. However, the δ18Ohemicellulose values (mean of the three sugars) do not correlate positively with δ18Oprec (r = -0.54, p fucose and xylose do not simply reflect δ18Oprec but rather δ18Oleaf water. The correlation between measured δ18Ohemicellulose and modeled δ18Oleaf water is highly significant (r = 0.81, p < 0.001, n = 20). This finding can be attributed to the evaporative 18O enrichment of leaf water during transpiration. Model sensitivity tests using a Péclet-modified Craig-Gordon (PMCG) model corroborate that relative air humidity is a very rigorous climate parameter influencing δ18Oleaf water, whereas temperature is of minor importance. While oxygen exchange and degradation effects on δ18O values of hemicelluloses sugar biomarkers seem to be negligible (Zech et al., 2012), further effects that need to be considered when interpreting δ18Ohemicellulose

  16. A rapid method to characterize seabed habitats and associated macro-organisms

    Science.gov (United States)

    Anderson, T.J.; Cochrane, G.R.; Roberts, D.A.; Chezar, H.; Hatcher, G.; ,

    2007-01-01

    This study presents a method for rapidly collecting, processing, and interrogating real-time abiotic and biotic seabed data to determine seabed habitat classifications. This is done from data collected over a large area of an acoustically derived seabed map, along multidirectional transects, using a towed small camera-sled. The seabed, within the newly designated Point Harris Marine Reserve on the northern coast of San Miguel Island, California, was acoustically imaged using sidescan sonar then ground-truthed using a towed small camera-sled. Seabed characterizations were made from video observations, and were logged to a laptop computer (PC) in real time. To ground-truth the acoustic mosaic, and to characterize abiotic and biotic aspects of the seabed, a three-tiered characterization scheme was employed that described the substratum type, physical structure (i.e., bedform or vertical relief), and the occurrence of benthic macrofauna and flora. A crucial advantage of the method described here, is that preliminary seabed characterizations can be interrogated and mapped over the sidescan mosaic and other seabed information within hours of data collection. This ability to rapidly process seabed data is invaluable to scientists and managers, particularly in modifying concurrent or planning subsequent surveys.

  17. Polychlorinated biphenyls (PCBs) in soils of the Moscow region: Concentrations and small-scale distribution along an urban-rural transect

    International Nuclear Information System (INIS)

    In soils of the Moscow region, we examined PCB concentrations in bulk samples and aggregate fractions. Topsoils under grassland and forest at five locations along a southeast-bound transect from Moscow and at a northeastern background location (grassland only) were analysed. We collected aggregates >1 cm and fractionated them into interior and exterior portions and also analysed the remaining soil without the aggregates >1 cm. The concentrations of the sum of 17 PCBs (Σ17PCBs) in 35 bulk soil samples ranged from 3.1 to 42 μg kg-1. This was 48-61% of the Σ33PCBs determined in 23 selected samples. The congeners 138(+158), 101 and 52 were most abundant. All PCB concentrations and the degree of chlorination declined with increasing distance from Moscow. The PCBs were accumulated in the aggregate exterior (on average 146% of the Σ17PCBs in bulk soil). We conclude that the ecotoxicological risk of PCBs in soils may not be properly assessed with the conventional bulk soil analysis. - PCBs are accumulated on aggregate surfaces where soil biological activity is particularly high

  18. Soil microbial respiration rate and temperature sensitivity along a north-south forest transect in eastern China: Patterns and influencing factors

    Science.gov (United States)

    Wang, Qing; He, Nianpeng; Yu, Guirui; Gao, Yang; Wen, Xuefa; Wang, Rongfu; Koerner, Sally E.; Yu, Qiang

    2016-02-01

    Soil organic matter is one of the most important carbon (C) pools in terrestrial ecosystems, and future warming from climate change will likely alter soil C storage via temperature effects on microbial respiration. In this study, we collected forest soils from eight locations along a 3700 km north-south transect in eastern China (NSTEC). For 8 weeks these soils were incubated under a periodically changing temperature range of 6-30°C while frequently measuring soil microbial respiration rate (Rs; each sample about every 20 min). This experimental design allowed us to investigate Rs and the temperature sensitivity of Rs (Q10) along the NSTEC. Both Rs at 20°C (R20) and Q10 significantly increased (logarithmically) with increasing latitude along the NSTEC suggesting that the sensitivity of soil microbial respiration to changing temperatures is higher in forest soils from locations with lower temperature. Our findings from an incubation experiment provide support for the hypothesis that temperature sensitivity of soil microbial respiration increases with biochemical recalcitrance (C quality-temperature hypothesis) across forest soils on a large spatial scale. Furthermore, microbial properties primarily controlled the observed patterns of R20, whereas both substrate and microbial properties collectively controlled the observed patterns of Q10. These findings advance our understanding of the driving factors (microbial versus substrate properties) of R20 and Q10 as well as the general relationships between temperature sensitivity of soil microbial respiration and environmental factors.

  19. Predicting available water of soil from particle-size distribution and bulk density in an oasis-desert transect in northwestern China

    Science.gov (United States)

    Li, Danfeng; Gao, Guangyao; Shao, Ming'an; Fu, Bojie

    2016-07-01

    A detailed understanding of soil hydraulic properties, particularly the available water content of soil, (AW, cm3 cm-3), is required for optimal water management. Direct measurement of soil hydraulic properties is impractical for large scale application, but routinely available soil particle-size distribution (PSD) and bulk density can be used as proxies to develop various prediction functions. In this study, we compared the performance of the Arya and Paris (AP) model, Mohammadi and Vanclooster (MV) model, Arya and Heitman (AH) model, and Rosetta program in predicting the soil water characteristic curve (SWCC) at 34 points with experimental SWCC data in an oasis-desert transect (20 × 5 km) in the middle reaches of the Heihe River basin, northwestern China. The idea of the three models emerges from the similarity of the shapes of the PSD and SWCC. The AP model, MV model, and Rosetta program performed better in predicting the SWCC than the AH model. The AW determined from the SWCCs predicted by the MV model agreed better with the experimental values than those derived from the AP model and Rosetta program. The fine-textured soils were characterized by higher AW values, while the sandy soils had lower AW values. The MV model has the advantages of having robust physical basis, being independent of database-related parameters, and involving subclasses of texture data. These features make it promising in predicting soil water retention at regional scales, serving for the application of hydrological models and the optimization of soil water management.

  20. The High Andean Cordillera of central Argentina and Chile along the Piuquenes Pass-Cordon del Portillo transect: Darwin's pioneering observations compared with modern geology

    Directory of Open Access Journals (Sweden)

    Laura Giambiagi

    2009-04-01

    Full Text Available The geological observations made by Darwin in 1835 during his crossing of the Andes from Santiago to Mendoza via the Piuquenes Pass and Cordón del Portillo are compared with the present geological knowledge of the Cordillera Principal and Cordillera Frontal at 33°-34°S. The analysis of the complex stratigraphy of the Cordillera Principal, the imbricated structure of the Aconcagua fold and thrust belt, as well as the stratigraphy and structure of the inter mountain foreland Tunuyán Basin, allows to assess the pioneer observations of Darwin. He recognized the old metamorphic basement and the granitoids and volcanic sequences of late Paleozoic to Triassic age of the Cordillera Frontal, established the Cretaceous age of the marine successions cropping out along the eastern Cordillera Principal and studied the conglomeratic deposits associated with the uplift of the Cordillera in the Alto Tunuyán Basin. Based on the study of clast provenance of the synorogenic deposits of the Alto Tunuyán Basin, Darwin recognized that the Cordillera Frontal was uplifted later than the Cordillera Principal. The present knowledge of this sector of the Andean Cordillera confirms his pioneer observations and show that Darwin was one of the first scientists ever in realizing that in an orogenic system the sequence of uplift and deformation proceeds from hinterland towards foreland, according to a process that is exceptionally well-illustrated along the Piuquenes-Cordón del Portillo transect.

  1. An outer ramp to basin plain transect: Interacting pelagic and calciturbidite deposition in the Eocene-Oligocene of the Tuscan Domain, Adria Microplate (Italy)

    Science.gov (United States)

    Ielpi, Alessandro; Cornamusini, Gianluca

    2013-08-01

    The interaction of ramps, basin plains and turbidite systems on the scale of tens of km has been rarely observed in fossil examples. Deep marine Eocene-Oligocene beds are exposed in the axial zone of the Chianti Mountains, Italy, and compose a regionally continue stratigraphic succession known as the Scaglia Toscana Formation. The formation was deposited in the Tuscan Domain of the Adria Microplate. This research aims at depicting its depositional architecture and evolution in the type area. Stratigraphic and sedimentologic analyses were performed on a ca. 25 km-long transect that includes depositional systems sectioned both in the down- and along-dip directions. Shaly-carbonate deposits compose a complex of interacting ramps, basin plains and turbidite floor fan systems. Ramp deposits accumulated above the lysocline and in oxic conditions. Basin plain beds were deposited below the lysocline and were subject to episodes of oxygen depletion. Turbidity flows fed elongate fan lobes characterized by poor channelisation. The basin palaeogeography hampered the development of slope apron turbidite systems. The Eocene-Oligocene geodynamic setting of the Tuscan Domain was characterized by the evolution of a peripheral bulge and by the early structuring of a foredeep basin. Syn-sedimentary tectonism acted a primary role in the basin-scale arrangement. However other mechanisms also contributed to the local facies distribution, including the disposition of sediment-source areas and intrabasinal confinement morphologies, as well as relative oscillations of the depositional surface with respect to the lysocline and oxycline.

  2. Life form-specific gradients in compound-specific hydrogen isotope ratios of modern leaf waxes along a North American Monsoonal transect.

    Science.gov (United States)

    Berke, Melissa A; Tipple, Brett J; Hambach, Bastian; Ehleringer, James R

    2015-12-01

    The use of hydrogen isotope ratios (δ(2)H) of sedimentary n-alkanes from leaf waxes has become an important tool for reconstructing paleoenvironmental and ancient hydrologic conditions. Studies of modern plant waxes can elucidate driving ecological mechanisms behind geologic deposits. Here, we used a transect across the North American Monsoon region of the western USA from Tucson, Arizona to Salt Lake City, Utah to study variations in leaf wax δ(2)H among co-occurring plants. Three co-occurring life forms were selected: perennial shrub (rabbit brush, Chrysothamnus nauseosus; sagebrush, Artemisia tridentata); tree (Gambel's oak tree, Quercus gambelii); and annual (sunflower, Helianthus annuus). Our results showed that the distributions and abundances of n-alkanes in perennial plants were similar across all sites and generally did not vary with environmental conditions (e.g., precipitation and temperature). In contrast, variations in n-alkane δ(2)H were significantly correlated with the fraction of the annual precipitation coming during the summer monsoon period. We use a modified Craig-Gordon model to speculate on the possible drivers of the δ(2)H values of leaf wax n-alkanes of plants across the region. The model results suggest that the most likely explanation for variation in wax δ(2)H values was a combination of seasonal source water usage and subsequent environmental conditions. PMID:26310435

  3. Evidence that dorsal locus coeruleus neurons can maintain their spinal cord projection following neonatal transection of the dorsal adrenergic bundle in rats.

    Science.gov (United States)

    Stanfield, B B

    1989-01-01

    In adult rats, locus coeruleus neurons which extend axons to the spinal cord are found only at mid-rostrocaudal levels of the nucleus, where they are essentially confined to its ventral, wedge-shaped half (Satoh et al. 1980; Westlund et al. 1983; Loughlin et al. 1986). However, during early postnatal development, coeruleospinal cells are found throughout the locus coeruleus (Cabana and Martin 1984; Chen and Stanfield 1987). This developmental restriction of the distribution of coeruleospinal neurons is due to axonal elimination rather than to cell death, since neurons retrogradely labeled through their spinal axons perinatally are still present in the dorsal portion of the locus coeruleus at survival periods beyond the age at which these cells lose their spinal projection (Chen and Stanfield 1987). I now report that if axons ascending from the locus coeruleus are cut by transecting the dorsal adrenergic bundle on the day of birth, a more widespread distribution of coeruleospinal neurons is retained beyond the perinatal period. These results not only indicate that the absence of the normally maintained collateral of a locus coeruleus neuron is sufficient to prevent the elimination of a collateral which would otherwise be lost, but also may imply that during normal postnatal development the presence of the maintained collateral is somehow causally involved in the elimination of the transient collateral. PMID:2612596

  4. Rapid diagnosis of bacteric meningitis

    Directory of Open Access Journals (Sweden)

    Sara Giordana Rimoldi

    2010-09-01

    Full Text Available Gram stain and bacterial antigen detection are economic and rapid tests for the identification of the bacteria in cerebrospinal fluid (CSF for the diagnosis of meningitis. Even if culture is the gold standard of these diagnosis, it requires long time (growth time. The purpose of our study was to compare in the laboratory diagnosis of meningitis real time PCR (Eurospital, Italy. with standard methods. Samples were collected in patients referred to L. Sacco Universital Hospital (range of age: 16 to 70 years from September 2008 to July 2009. 9 out of 130 CFS samples investigated were positive in PCR but they produced different results to the other methods used.

  5. Rapidly Adaptable Instrumentation Tester (RAIT)

    International Nuclear Information System (INIS)

    Emerging technologies in the field of ''Test ampersand Measurement'' have recently enabled the development of the Rapidly Adaptable Instrumentation Tester (RAIT). Based on software developed with LabVIEW, the RAIT design enables quick reconfiguration to test and calibrate a wide variety of telemetry systems. The consequences of inadequate testing could be devastating if a telemetry system were to fail during an expensive flight mission. Supporting both open-bench testing as well as automated test sequences, the RAIT has significantly lowered total time required to test and calibrate a system. This has resulted in an overall lower per unit testing cost than has been achievable in the past

  6. 2008年春季中国南方冰雪冻害林木折损分析%Analysis of forest damage caused by the snow and ice chaos along a transect across southern China in spring 2008

    Institute of Scientific and Technical Information of China (English)

    邵全琴; 黄麟; 刘纪远; 匡文慧; 李佳

    2011-01-01

    An abrupt ice and snow storm disaster which occurred in the spring of 2008 severely destroyed forests over a surprisingly large portion of southern China. A transect crossing Jinggang Mountain-Jitai Basin-Yushan Mountain-Wuyi Mountain was selected as the study area. The authors integrated field data collected in two field surveys to analyze the impacts of the disturbance on forests. The following results were obtained. (1) The extent of damage to plantations along the transect decreased in the order of slash pine > masson pine > mixed plantation > Chinese fir. Slash pine is an introduced species from southern America which is characterized by fast growth, low wood quality and rich oleoresin, and showed a damage rate of 61.3% of samples, of which 70.4% cannot recover naturally. Masson pine is the native pioneer species of forests with harder wood, and 52.5% were damaged due to turpentine, of which 60.9% cannot recovery naturally. Chinese fir is a local tree species and samples showed a rate of 46% and a relative rate of 32.5%, lower than the mixed plantation.(2) From west to east along the transect, we can see that evergreen broad-leaved forest of the western transect on Jinggang Mountain showed the lightest damage extent, and a Cryptomeria plantation at an altitude of 700 m was severely destroyed while Chinese fir showed light damage below 700 m and relatively severe damage above 900 m. Masson pine and slash pine in the central transect in Jitai Basin were damaged severely due to turpentine activities, and closed natural secondary deciduous broad-leaved forest was damaged severely due to high ice and snow accumulation on intertwined shrubs. Masson pine aerial-seeding plantations below 400 m along the eastern transect in Xingguo and Ningdu counties were nearly undamaged for small tree sizes, and Chinese fir at 500-900 m altitude showed a lighter damage extent. However, masson pine which was distributed above 400 m and planted in the 1960s, was

  7. Rapidly rotating neutron star progenitors

    CERN Document Server

    Postnov, K A; Kolesnikov, D A; Popov, S B; Porayko, N K

    2016-01-01

    Rotating proto-neutron stars can be important sources of gravitational waves to be searched for by present-day and future interferometric detectors. It was demonstrated by Imshennik that in extreme cases the rapid rotation of a collapsing stellar core may lead to fission and formation of a binary proto-neutron star which subsequently merges due to gravitational wave emission. In the present paper, we show that such dynamically unstable collapsing stellar cores may be the product of a former merger process of two stellar cores in a common envelope. We applied population synthesis calculations to assess the expected fraction of such rapidly rotating stellar cores which may lead to fission and formation of a pair of proto-neutron stars. We have used the BSE population synthesis code supplemented with a new treatment of stellar core rotation during the evolution via effective core-envelope coupling, characterized by the coupling time, $\\tau_c$. The validity of this approach is checked by direct MESA calculations ...

  8. Rapidly rotating neutron star progenitors

    Science.gov (United States)

    Postnov, K. A.; Kuranov, A. G.; Kolesnikov, D. A.; Popov, S. B.; Porayko, N. K.

    2016-08-01

    Rotating proto-neutron stars can be important sources of gravitational waves to be searched for by present-day and future interferometric detectors. It was demonstrated by Imshennik that in extreme cases the rapid rotation of a collapsing stellar core may lead to fission and formation of a binary proto-neutron star which subsequently merges due to gravitational wave emission. In the present paper, we show that such dynamically unstable collapsing stellar cores may be the product of a former merger process of two stellar cores in a common envelope. We applied population synthesis calculations to assess the expected fraction of such rapidly rotating stellar cores which may lead to fission and formation of a pair of proto-neutron stars. We have used the BSE population synthesis code supplemented with a new treatment of stellar core rotation during the evolution via effective core-envelope coupling, characterized by the coupling time, τc. The validity of this approach is checked by direct MESA calculations of the evolution of a rotating 15 M⊙ star. From comparison of the calculated spin distribution of young neutron stars with the observed one, reported by Popov and Turolla, we infer the value τc ≃ 5 × 105 years. We show that merging of stellar cores in common envelopes can lead to collapses with dynamically unstable proto-neutron stars, with their formation rate being ˜0.1 - 1% of the total core collapses, depending on the common envelope efficiency.

  9. Rapid ISS Power Availability Simulator

    Science.gov (United States)

    Downing, Nicholas

    2011-01-01

    The ISS (International Space Station) Power Resource Officers (PROs) needed a tool to automate the calculation of thousands of ISS power availability simulations used to generate power constraint matrices. Each matrix contains 864 cells, and each cell represents a single power simulation that must be run. The tools available to the flight controllers were very operator intensive and not conducive to rapidly running the thousands of simulations necessary to generate the power constraint data. SOLAR is a Java-based tool that leverages commercial-off-the-shelf software (Satellite Toolkit) and an existing in-house ISS EPS model (SPEED) to rapidly perform thousands of power availability simulations. SOLAR has a very modular architecture and consists of a series of plug-ins that are loosely coupled. The modular architecture of the software allows for the easy replacement of the ISS power system model simulator, re-use of the Satellite Toolkit integration code, and separation of the user interface from the core logic. Satellite Toolkit (STK) is used to generate ISS eclipse and insulation times, solar beta angle, position of the solar arrays over time, and the amount of shadowing on the solar arrays, which is then provided to SPEED to calculate power generation forecasts. The power planning turn-around time is reduced from three months to two weeks (83-percent decrease) using SOLAR, and the amount of PRO power planning support effort is reduced by an estimated 30 percent.

  10. KEPLER RAPIDLY ROTATING GIANT STARS

    Energy Technology Data Exchange (ETDEWEB)

    Costa, A. D.; Martins, B. L. Canto; Bravo, J. P.; Paz-Chinchón, F.; Chagas, M. L. das; Leão, I. C.; Oliveira, G. Pereira de; Silva, R. Rodrigues da; Roque, S.; Oliveira, L. L. A. de; Silva, D. Freire da; De Medeiros, J. R., E-mail: renan@dfte.ufrn.br [Departamento de Física Teórica e Experimental, Universidade Federal do Rio Grande do Norte, Campus Universitário, Natal RN (Brazil)

    2015-07-10

    Rapidly rotating giant stars are relatively rare and may represent important stages of stellar evolution, resulting from stellar coalescence of close binary systems or accretion of substellar companions by their hosting stars. In the present Letter, we report 17 giant stars observed in the scope of the Kepler space mission exhibiting rapid rotation behavior. For the first time, the abnormal rotational behavior for this puzzling family of stars is revealed by direct measurements of rotation, namely from photometric rotation period, exhibiting a very short rotation period with values ranging from 13 to 55 days. This finding points to remarkable surface rotation rates, up to 18 times the rotation of the Sun. These giants are combined with six others recently listed in the literature for mid-infrared (IR) diagnostics based on Wide-field Infrared Survey Explorer information, from which a trend for an IR excess is revealed for at least one-half of the stars, but at a level far lower than the dust excess emission shown by planet-bearing main-sequence stars.

  11. Kepler rapidly rotating giant stars

    CERN Document Server

    Costa, A D; Bravo, J P; Paz-Chinchón, F; Chagas, M L das; Leão, I C; de Oliveira, G Pereira; da Silva, R Rodrigues; Roque, S; de Oliveira, L L A; da Silva, D Freire; De Medeiros, J R

    2015-01-01

    Rapidly rotating giant stars are relatively rare and may represent important stages of stellar evolution, resulting from stellar coalescence of close binary systems or accretion of sub-stellar companions by their hosting stars. In the present letter we report 17 giant stars observed in the scope of the Kepler space mission exhibiting rapid rotation behavior. For the first time the abnormal rotational behavior for this puzzling family of stars is revealed by direct measurements of rotation, namely from photometric rotation period, exhibiting very short rotation period with values ranging from 13 to 55 days. This finding points for remarkable surface rotation rates, up to 18 times the Sun rotation. These giants are combined with 6 other recently listed in the literature for mid-IR diagnostic based on WISE information, from which a trend for an infrared excess is revealed for at least a half of the stars, but at a level far lower than the dust excess emission shown by planet-bearing main-sequence stars.

  12. Rapid self-healing hydrogels

    Science.gov (United States)

    Phadke, Ameya; Zhang, Chao; Arman, Bedri; Hsu, Cheng-Chih; Mashelkar, Raghunath A.; Lele, Ashish K.; Tauber, Michael J.; Arya, Gaurav; Varghese, Shyni

    2012-01-01

    Synthetic materials that are capable of autonomous healing upon damage are being developed at a rapid pace because of their many potential applications. Despite these advancements, achieving self-healing in permanently cross-linked hydrogels has remained elusive because of the presence of water and irreversible cross-links. Here, we demonstrate that permanently cross-linked hydrogels can be engineered to exhibit self-healing in an aqueous environment. We achieve this feature by arming the hydrogel network with flexible-pendant side chains carrying an optimal balance of hydrophilic and hydrophobic moieties that allows the side chains to mediate hydrogen bonds across the hydrogel interfaces with minimal steric hindrance and hydrophobic collapse. The self-healing reported here is rapid, occurring within seconds of the insertion of a crack into the hydrogel or juxtaposition of two separate hydrogel pieces. The healing is reversible and can be switched on and off via changes in pH, allowing external control over the healing process. Moreover, the hydrogels can sustain multiple cycles of healing and separation without compromising their mechanical properties and healing kinetics. Beyond revealing how secondary interactions could be harnessed to introduce new functions to chemically cross-linked polymeric systems, we also demonstrate various potential applications of such easy-to-synthesize, smart, self-healing hydrogels. PMID:22392977

  13. Rapidly Developing Toxic Epidermal Necrolysis

    Directory of Open Access Journals (Sweden)

    Viktoria Oline Barrios Poulsen

    2013-01-01

    Full Text Available Severe cutaneous reactions with potentially fatal outcomes can have many different causes. The Stevens-Johnson syndrome (SJS and toxic epidermal necrolysis (TEN are rare. They are characterized by a low incidence but high mortality, and drugs are most commonly implicated. Urgent active therapy is required. Prompt recognition and withdrawal of suspect drug and rapid intervention can result in favourable outcome. No further international guidelines for treatment exist, and much of the treatment relies on old or experimental concepts with no scientific evidence. We report on a 54-year-old man experiencing rapidly developing drug-induced severe TEN and presented multiorgan failure involving the respiratory and circulatory system, coagulopathy, and renal insufficiency. Detachment counted 30% of total body surface area (TBSA. SCORTEN = 5, indicating a mortality rate >90%. The patient was sedated and mechanically ventilated, supported with fluids and inotropes to maintain a stable circulation. Component therapy was guided by thromboelastography (TEG. The patient received plasmapheresis, and shock reversal treatment was initiated. He was transferred to a specialized intensive care burn unit within 24 hours from admittance. The initial care was continued, and hemodialysis was started. Pulmonary, circulatory, and renal sequelae resolved with intensive care, and re-epithelialization progressed slowly. The patient was discharged home on hospital day 19.

  14. Moved by a Rapid Transit

    Science.gov (United States)

    Bueter, C.

    2013-04-01

    Enticing by virtue of its predictability, historical utility, and spectacle, the transit of Venus is a niche event among astronomical phenomena. Though the value of a transit for scientific purposes is now diminished, the brief appearance of Venus silhouetted against the background of the Sun in 2004 moved the artistic community to celebrate the rare alignment. Artists of all ages combined old traditions with fresh technology to create a 21st-century tapestry of music, sculpture, paintings, glasswork, quilts, sky shows, and digital imagery. A full catalog of transit-related art generated over the centuries would feature the sampling of entries presented here and at the Moved by a Rapid Transit website.

  15. Rapid world modelling for robotics

    International Nuclear Information System (INIS)

    The ability to use an interactive world model, whether it is for robotics simulation or most other virtual graphical environments, relies on the users ability to create an accurate world model. Typically this is a tedious process, requiring many hours to create 3-D CAD models of the surfaces within a workspace. The goal of this ongoing project is to develop usable methods to rapidly build world models of real world workspaces. This brings structure to an unstructured environment and allows graphical based robotics control to be accomplished in a reasonable time frame when traditional CAD modelling is not enough. To accomplish this, 3D range sensors are deployed to capture surface data within the workspace. This data is then transformed into surface maps, or models. A 3D world model of the workspace is built quickly and accurately, without ever having to put people in the environment

  16. Rapid Adaptation in Digital Transformation

    DEFF Research Database (Denmark)

    Hansen, Anne Mette; Kræmmergaard, Pernille; Mathiassen, Lars

    2011-01-01

    In today’s highly dynamic environments, organizational leaders need to quickly adapt existing approaches to digital transformation. However, without a shared mindset between IS and business leaders, it is difficult to adopt new approaches in response to changes in the competitive and technology...... landscape. In this article, we share insights gained from two public sector organizations in which IS and business leaders used the Participatory Process Model (PPM) designed by the authors to share their assumptions about IS leadership, challenge existing IT strategies and collaboration patterns and adapt...... the organization’s digitization approach. We demonstrate in detail how the leaders within these two organizations were engaged and offer recommendations for how other organizations can use the PPM to rapidly adapt their approaches to digital transformation through more effective IS leadership roles....

  17. Rapid adaptation to climate change.

    Science.gov (United States)

    Hancock, Angela M

    2016-08-01

    In recent years, amid growing concerns that changing climate is affecting species distributions and ecosystems, predicting responses to rapid environmental change has become a major goal. In this issue, Franks and colleagues take a first step towards this objective (Franks et al. 2016). They examine genomewide signatures of selection in populations of Brassica rapa after a severe multiyear drought. Together with other authors, Franks had previously shown that flowering time was reduced after this particular drought and that the reduction was genetically encoded. Now, the authors have sequenced previously stored samples to compare allele frequencies before and after the drought and identify the loci with the most extreme shifts in frequencies. The loci they identify largely differ between populations, suggesting that different genetic variants may be responsible for reduction in flowering time in the two populations. PMID:27463237

  18. Silicone:Worries behind Rapid Growth

    Institute of Scientific and Technical Information of China (English)

    2007-01-01

    @@ Rapid consumption growth Silicone's extensive application scope, sustained production growth and constant technology improvements have benefited from the rapid development of China's economy overall, and particularly the boom in the manufacturing sector.

  19. Platform-induced clay-mineral fractionation along a northern Tethyan basin-platform transect: implications for the interpretation of Early Cretaceous climate change (Late Hauterivian-Early Aptian)

    OpenAIRE

    Godet, Alexis; Bodin, Stéphane; Adatte, Thierry; Föllmi, Karl B.

    2009-01-01

    High-resolution clay-mineral analyses were performed on upper Hauterivian to lower Aptian sediments along a platform-to-basin transect through the northern Tethyan margin from the Neuchâtel area (Switzerland), to the Vocontian Trough (France) in order to investigate links between climate change, carbonate platform evolution, and fractionation patterns in clay minerals during their transport. During the Hauterivian, the northern Tethyan carbonate platform developed in a heterozoan mode, and t...

  20. Effects of bone morphogenetic protein-4 on spatial memory and cholinergic expression in the dentate gyrus after fornix-fimbria transection in rats

    Institute of Scientific and Technical Information of China (English)

    Lei Liu; Yilong Xue; Jingkun Pan; Yazhuo Hu; Yuhong Gao; Yun Luo

    2008-01-01

    BACKGROUND: Previous experiments have confirmed bone morphogenetic proteins (BMPs) upregulate cholinergic expression in neurons isolated from the embryonic rat hippocampus and cerebral cortex. Therefore, BMPs could be useful for treating Alzheimer's disease and other neurodegenerative diseases. OBJECTIVE: BMP-4 was infused into the hippocampal dentate gyrus of fornix-fimbria transected rats to test the effects of BMP-4 on cholinergic expression in dentate gyrus neurons, and to observe changes in spatial memory behavior. DESIGN: A randomized controlled animal experiment. SETTING: Department of Neurosurgery and Laboratory for Cell Biology, Institute of Geriatrics, General Hospital of Chinese PLA.MATERIALS: Twenty-seven healthy adult male Sprague Dawley (SD) rats, weighing 250-300 g, were provided by the Laboratory Animal Center of the General Hospital of Chinese PLA. Reagents: BMP-4 (B-2680, Sigma Company) and choline acetyl transferase (ChAT) antibody (AB5042, Chemicon Company) were used in this study. Equipments: a rat stereotaxic instrument (type: SN-2N, Narushige Group, Japan) and Image-prog-plus image analysis software (Media Cybernetics company, USA) were used in this study. The protocol was carried out in accordance with ethical guidelines for the use and care of animals.METHODS: This experiment was performed in the Institute of Geriatrics, General Hospital of Chinese PLA between July 2004 and March 2005. Rats were randomly divided into 4 groups: Alzheimer's disease group (n = 7), normal control group (n = 5), BMP-4-Alzheimer's disease group (n = 8), and model group (n = 7). In the Alzheimer's disease group, the left hippocampal fornix-fimbria of rats was transected to mimic Alzheimer's disease symptoms. In the BMP-4-Alzheimer's disease group, 1 μL BMP-4 (10 mg/L) was perfused into the left dentate gyrus with a microinjector at 1 μL/min. In the model group, 1 μL saline was perfused into the same position by the same method. Twenty-eight days after injection

  1. Autocrine fibronectin from differentiating mesenchymal stem cells induces the neurite elongation in vitro and promotes nerve fiber regeneration in transected spinal cord injury.

    Science.gov (United States)

    Zeng, Xiang; Ma, Yuan-Huan; Chen, Yuan-Feng; Qiu, Xue-Cheng; Wu, Jin-Lang; Ling, Eng-Ang; Zeng, Yuan-Shan

    2016-08-01

    Extracellular matrix (ECM) expression is temporally and spatially regulated during the development of stem cells. We reported previously that fibronectin (FN) secreted by bone marrow mesenchymal stem cells (MSCs) was deposited on the surface of gelatin sponge (GS) soon after culture. In this study, we aimed to assess the function of accumulated FN on neuronal differentiating MSCs as induced by Schwann cells (SCs) in three dimensional transwell co-culture system. The expression pattern and amount of FN of differentiating MSCs was examined by immunofluorescence, Western blot and immunoelectron microscopy. The results showed that FN accumulated inside GS scaffold, although its mRNA expression in MSCs was progressively decreased during neural induction. MSC-derived neuron-like cells showed spindle-shaped cell body and long extending processes on FN-decorated scaffold surface. However, after blocking of FN function by application of monoclonal antibodies, neuron-like cells showed flattened cell body with short and thick neurites, together with decreased expression of integrin β1. In vivo transplantation study revealed that autocrine FN significantly facilitated endogenous nerve fiber regeneration in spinal cord transection model. Taken together, the present results showed that FN secreted by MSCs in the early stage accumulated on the GS scaffold and promoted the neurite elongation of neuronal differentiating MSCs as well as nerve fiber regeneration after spinal cord injury. This suggests that autocrine FN has a dynamic influence on MSCs in a three dimensional culture system and its potential application for treatment of traumatic spinal cord injury. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 104A: 1902-1911, 2016. PMID:26991461

  2. Five-years of microenvironment data along an urban-rural transect; temperature and CO2 concentrations in urban area at levels expected globally with climate change.

    Energy Technology Data Exchange (ETDEWEB)

    George, Kate; Ziska, Lewis H; Bunce, James A; Quebedeaux, Bruno

    2007-11-01

    The heat island effect and the high use of fossil fuels in large city centers is well documented, but by how much fossil fuel consumption is elevating atmospheric CO2 concentrations and whether elevations in both atmospheric CO2 and air temperature are consistent from year to year are less well known. Our aim was to record atmospheric CO2 concentrations, air temperature and other environmental variables in an urban area and compare it to suburban and rural sites to see if urban sites are experiencing climates expected globally in the future with climate change. A transect was established from Baltimore city center (Urban site), to the outer suburbs of Baltimore (suburban site) and out to an organic farm (rural site). At each site a weather station was set-up to monitor environmental variables annually for five years. Atmospheric CO2 was significantly increased on average by 66 ppm from the rural to the urban site over the five years of the study. Air temperature was significantly higher at the urban site (14.8 oC) compared to the suburban (13.6 oC) and rural (12.7 oC) sites. Relative humidity was not different between sites but vapor pressure deficit (VPD) was significantly higher at the urban site compared to the suburban and rural sites. During wet years relative humidity was significantly increased and VPD significantly reduced. Increased nitrogen deposition at the rural site (2.1 % compared to 1.8 and 1.2 % at the suburban and urban sites) was small enough not to affect soil nitrogen content. Dense urban areas with large populations and high vehicular traffic have significantly different microclimates compared to outlying suburban and rural areas. The increases in atmospheric CO2 and air temperature are similar to changes predicted in the short term with global climate change, therefore providing an environment suitable for studying future effects of climate change on terrestrial ecosystems.

  3. Regional structure of the southern segment of Central Iberian Zone (Spanish Variscan Belt) interpreted from potential field images and 2.5 D modelling of Alcudia gravity transect

    Science.gov (United States)

    García-Lobón, J. L.; Rey-Moral, C.; Ayala, C.; Martín-Parra, L. M.; Matas, J.; Reguera, M. I.

    2014-02-01

    This study presents a structural interpretation of potential field data at the southern segment of the Spanish Central Iberian Zone, where little is known about its geometry and physical properties in depth. We used ground gravity and aeromagnetic data from the Spanish Geological Survey (IGME) databases, together with a gravity survey recently acquired along the Alcudia deep seismic reflection profile, and also petrophysical data from rock samples, in part specifically acquired for this work. The aim is to characterize with potential fields the depth structure and physical properties of a complex area, focusing on features of the upper crust where the potential fields can provide a useful insight, as these features appear to be transparent in the images of seismic surveys carried up to date. After building a 2.5 D model of the magnetic susceptibility and density distribution for the whole crust with the constraints of the Alcudia seismic transect, relationships between lower and upper crustal structures can be better established, and surface geophysical maps allow estimating the lateral extensions of the main structures characterized by the potential field modelling. Interpreted gravity and magnetic Alcudia features consist of: 1) long-wavelength anomalies due to changes in crustal thickness and structure, identified along the Alcudia seismic reflection profile; some crustal boundaries and the seismic Moho have been slightly modified in the potential field model to fit the observed data; and, 2) short wavelength anomalies related to upper crustal features as large NW-SE faults and folds, and plutonic alignments. Northern and southern Alcudia segments show relevant lower-middle crust shortening structures underneath extensive Variscan plutons (Mora, Pedroches) and folded basins (Pedroches). Potential field imaging and modelling of these structures have provided a better understanding of the subsurface geology, particularly in non-reflective areas, unravelling the

  4. Are mangroves drivers or buffers of coastal acidification? Insights from alkalinity and dissolved inorganic carbon export estimates across a latitudinal transect

    Science.gov (United States)

    Sippo, James Z.; Maher, Damien T.; Tait, Douglas R.; Holloway, Ceylena; Santos, Isaac R.

    2016-05-01

    Mangrove forests are hot spots in the global carbon cycle, yet the fate for a majority of mangrove net primary production remains unaccounted for. The relative proportions of alkalinity and dissolved CO2 [CO2*] within the dissolved inorganic carbon (DIC) exported from mangroves is unknown, and therefore, the effect of mangrove DIC exports on coastal acidification remains unconstrained. Here we measured dissolved inorganic carbon parameters over complete tidal and diel cycles in six pristine mangrove tidal creeks covering a 26° latitudinal gradient in Australia and calculated the exchange of DIC, alkalinity, and [CO2*] between mangroves and the coastal ocean. We found a mean DIC export of 59 mmol m-2 d-1 across the six systems, ranging from import of 97 mmol m-2 d-1 to an export of 85 mmol m-2 d-1. If the Australian transect is representative of global mangroves, upscaling our estimates would result in global DIC exports of 3.6 ± 1.1 Tmol C yr-1, which accounts for approximately one third of the previously unaccounted for mangrove carbon sink. Alkalinity exchange ranged between an import of 1.2 mmol m-2 d-1 and an export of 117 mmol m-2 d-1 with an estimated global export of 4.2 ± 1.3 Tmol yr-1. A net import of free CO2 was estimated (-11.4 ± 14.8 mmol m-2 d-1) and was equivalent to approximately one third of the air-water CO2 flux (33.1 ± 6.3 mmol m-2 d-1). Overall, the effect of DIC and alkalinity exports created a measurable localized increase in coastal ocean pH. Therefore, mangroves may partially counteract coastal acidification in adjacent tropical waters.

  5. The effects of post-pasture woody plant colonization on soil and aboveground litter carbon and nitrogen along a bioclimatic transect

    Directory of Open Access Journals (Sweden)

    La Mantia T

    2013-06-01

    Full Text Available We investigated the effects of woody plant colonization of abandoned pastures on soil and litter organic carbon (C stocks and nitrogen (N content along a bioclimatic transect in a semi-arid environment (Sicily, Italy. Soil samples were taken in three successional stages (grazed pasture, shrubland, forest within each of three bioclimates (supramediterranean - “supra”, mesomediterranean - “meso”, thermomediterranean - “thermo”. Organic C and N in litter and soil (0-10 cm and 10-30 cm depth were determined, as well as soil bulk density. Especially at 0-10 cm depth, changes in C and N contents along successional stages differed among bioclimates. Soil organic carbon (SOC stock decreased from pasture to shrubland and increased from shrubland to forest in “supra”, increased from pasture to shrubland and then remained stable in “thermo”, and was stable in “meso”. Soil C/N ratio decreased with succession in “supra”, showed no significant trend in “meso”, and increased with succession in “thermo”. Litter C stock increased with succession in “meso”, increased from pasture to shrubland and decreased from shrubland to forest in “thermo”, and increased from pasture to shrubland and then remained stable in “supra”. Litter C/N ratio increased in “thermo” and “supra” from pasture to shrubland and from shrubland to forest, but did not change significantly with succession in “meso”. The different trends in SOC among bioclimates may be caused by changes in the importance of litter input, litter decay rate and mineralization. Successional changes in “meso” and “supra” appeared to be most affected by litter quality, while those in “thermo” appeared to be strongly influenced by limited litter decay due to low soil moisture and high temperature.

  6. The Use of Bovine Pericardial Buttress on Linear Stapler Fails to Reduce Pancreatic Fistula Incidence in a Porcine Pancreatic Transection Model

    Directory of Open Access Journals (Sweden)

    A. Maciver

    2011-01-01

    Full Text Available We investigate the effectiveness of buttressing the surgical stapler to reduce postoperative pancreatic fistulae in a porcine model. As a pilot study, pigs (n=6 underwent laparoscopic distal pancreatectomy using a standard stapler. Daily drain output and lipase were measured postoperative day 5 and 14. In a second study, pancreatic transection was performed to occlude the proximal and distal duct at the pancreatic neck using a standard stapler (n=6, or stapler with bovine pericardial strip buttress (n=6. Results. In pilot study, 3/6 animals had drain lipase greater than 3x serum on day 14. In the second series, drain volumes were not significantly different between buttressed and control groups on day 5 (55.3 ± 31.6 and 29.3 ± 14.2 cc, resp., nor on day 14 (9.5 ± 4.2 cc and 2.5 ± 0.8 cc, resp., P=0.13. Drain lipase was not statistically significant on day 5 (3,166 ± 1,433 and 6,063 ± 1,872 U/L, resp., P=0.25 or day 14 (924 ± 541 and 360 ± 250 U/L. By definition, 3/6 developed pancreatic fistula; only one (control demonstrating a contained collection arising from the staple line. Conclusion. Buttressed stapler failed to protect against pancreatic fistula in this rigorous surgical model.

  7. Rabbit Achilles tendon full transection model - wound healing, adhesion formation and biomechanics at 3, 6 and 12 weeks post-surgery.

    Science.gov (United States)

    Meier Bürgisser, Gabriella; Calcagni, Maurizio; Bachmann, Elias; Fessel, Gion; Snedeker, Jess G; Giovanoli, Pietro; Buschmann, Johanna

    2016-01-01

    After tendon rupture repair, two main problems may occur: re-rupture and adhesion formation. Suitable non-murine animal models are needed to study the healing tendon in terms of biomechanical properties and extent of adhesion formation. In this study 24 New Zealand White rabbits received a full transection of the Achilles tendon 2 cm above the calcaneus, sutured with a 4-strand Becker suture. Post-surgical analysis was performed at 3, 6 and 12 weeks. In the 6-week group, animals received a cast either in a 180 deg stretched position during 6 weeks (adhesion provoking immobilization), or were re-casted with a 150 deg position after 3 weeks (adhesion inhibiting immobilization), while in the other groups (3 and 12 weeks) a 180 deg position cast was applied for 3 weeks. Adhesion extent was analyzed by histology and ultrasound. Histopathological scoring was performed according to a method by Stoll et al. (2011), and the main biomechanical properties were assessed. Histopathological scores increased as a function of time, but did not reach values of healthy tendons after 12 weeks (only around 15 out of 20 points). Adhesion provoking immobilization led to an adhesion extent of 82.7±9.7%, while adhesion inhibiting immobilization led to 31.9±9.8% after 6 weeks. Biomechanical properties increased over time, however, they did not reach full strength nor elastic modulus at 12 weeks post-operation. Furthermore, the rabbit Achilles tendon model can be modulated in terms of adhesion formation to the surrounding tissue. It clearly shows the different healing stages in terms of histopathology and offers a suitable model regarding biomechanics because it exhibits similar biomechanics as the human flexor tendons of the hand. PMID:27635037

  8. Spatial variations of community structures and methane cycling across a transect of Lei-Gong-Hou mud volcanoes in eastern Taiwan

    Directory of Open Access Journals (Sweden)

    Pei-Ling eWang

    2014-03-01

    Full Text Available This study analyzed cored sediments retrieved from sites distributed across a transect of the Lei-Gong-Hou mud volcanoes in eastern Taiwan to uncover the spatial distributions of biogeochemical processes and community assemblages involved in methane cycling. The profiles of methane concentration and carbon isotopic composition revealed various orders of the predominance of specific methane-related metabolisms along depth. At a site proximal to the bubbling pool, the methanogenic zone was sandwiched by the anaerobic methanotrophic zones. For two sites distributed toward the topographic depression, the methanogenic zone overlaid the anaerobic methanotrophic zone. The predominance of anaerobic methanotrophy at specific depth intervals is supported by the enhanced copy numbers of the ANME-2a 16S rRNA gene and coincides with high dissolved Fe/Mn concentrations and copy numbers of the Desulfuromonas/Pelobacter 16S rRNA gene. Assemblages of 16S rRNA and mcrA genes revealed that methanogenesis was mediated by Methanococcoides and Methanosarcina. pmoA genes and a few 16S rRNA genes related to aerobic methanotrophs were detected in limited numbers of subsurface samples. While dissolved Fe/Mn signifies the presence of anaerobic metabolisms near the surface, the correlations between geochemical characteristics and gene abundances, and the absence of aerobic methanotrophs in top sediments suggest that anaerobic methanotrophy is potentially dependent on iron/manganese reduction and dominates over aerobic methanotrophy for the removal of methane produced in situ or from a deep source. Near-surface methanogenesis contributes to the methane emissions from mud platform. The alternating arrangements of methanogenic and methanotrophic zones at different sites suggest that the interactions between mud deposition, evaporation, oxidation and fluid transport modulate the assemblages of microbial communities and methane cycling in different compartments of terrestrial

  9. Variability of magnetic character of S5-1 paleosol (age ∼470 Ka) along a rainfall transect explains why susceptibility decreased with high rainfall

    Science.gov (United States)

    Guo, Xuelian; Liu, Xiuming; Miao, Shengjun; Zhao, Guoyong; Liu, Yixin

    2015-12-01

    This study was based mainly on environmental magnetic, soil science and geochemical methods to analyze the spatial variation of magnetic characteristics of the S5-1 paleosol (age ∼470 Ka) in a NW-SE transect from Xifeng, Linyou and Baoji sections from the Chinese Loess Plateau. The results show that (a) pedogenesis and chemical weathering of the coeval S5-1 paleosol layers increased with rainfall from Xifeng to Baoji, (b) the proportion of the pedogenic maghemite component decreased, while (c) the concentrations of weakly magnetic antiferromagnetic minerals (hematite and goethite) relatively increased. Maghemite and hematite appear to have formed simultaneously with development of pedogenesis, thus being similar in this aspect to soil in arid regions of SW Spain. However, amount of goethite did not show a consistent relationship as observed from magnetism in the three sections. Maghemite was found to be the main contributor to magnetic enhancement. Iron-manganese cutans, an indicator of diagnesis, increased with the rainfall in the S5-1 paleosol layer from Xifeng to Baoji. As expected, they show that pedogenesis occurred intermittently between wet and dry conditions causing iron mineral transformation. However, we suggest that parts of the fine-grained strongly magnetic minerals (mainly oxidized magnetite and maghemite) were converted into weakly magnetic minerals (mainly hematite and goethite) by pedogenesis, which resulted in a decline in the finest superparamagnetic (SP) and stable single-domain (SSD) ferrimagnetic minerals, thus decreasing the susceptibility of S5-1 paleosol from north to south. This behavior in a high rainfall region is different from the aerobic soil in SW Spain in which part of the initial hydro-maghemite transformed only to hematite rather than goethite and hematite.

  10. Knockdown of α-synuclein in cerebral cortex improves neural behavior associated with apoptotic inhibition and neurotrophin expression in spinal cord transected rats.

    Science.gov (United States)

    Wang, You-Cui; Feng, Guo-Ying; Xia, Qing-Jie; Hu, Yue; Xu, Yang; Xiong, Liu-Lin; Chen, Zhi-Wei; Wang, Hang-Ping; Wang, Ting-Hua; Zhou, Xue

    2016-04-01

    Spinal cord injury (SCI) often causes severe functional impairment with poor recovery. The treatment, however, is far from satisfaction, and the mechanisms remain unclear. By using proteomics and western blot, we found spinal cord transection (SCT) resulted in a significant down-regulation of α-synuclein (SNCA) in the motor cortex of SCT rats at 3 days post-operation. In order to detect the role of SNCA, we used SNCA-ORF/shRNA lentivirus to upregulate or knockdown SNCA expression. In vivo, SNCA-shRNA lentivirus injection into the cerebral cortex motor area not only inhibited SNCA expression, but also significantly enhanced neurons' survival, and attenuated neuronal apoptosis, as well as promoted motor and sensory function recovery in hind limbs. While, overexpression SNCA exhibited the opposite effects. In vitro, cortical neurons transfected with SNCA-shRNA lentivirus gave rise to an optimal neuronal survival and neurite outgrowth, while it was accompanied by reverse efficiency in SNCA-ORF group. In molecular level, SNCA silence induced the upregulation of Bcl-2 and the downregulation of Bax, and the expression of NGF, BDNF and NT3 was substantially upregulated in cortical neurons. Together, endogenous SNCA play a crucial role in motor and sensory function regulation, in which, the underlying mechanism may be linked to the regulation of apoptosis associated with apoptotic gene (Bax, Bcl2) and neurotrophic factors expression (NGF, BDNF and NT3). These finds provide novel insights to understand the role of SNCA in cerebral cortex after SCT, and it may be as a novel treatment target for SCI repair in future clinic trials. PMID:26822976

  11. Experimental micropedology—A technique for investigating soil carbonate biogenesis along a desert-grassland-forest transect, New Mexico, USA

    Directory of Open Access Journals (Sweden)

    Farhad Khormali

    2014-03-01

    Full Text Available Manipulative experiments-characterized by the comparing treatments to controls-are widespread in scientific investigations. This study uses experimental micropedology to investigate whether soil microbes precipitate carbonate if a liquid growth-medium is applied to soil in situ. This was undertaken using apparatuses designed to (1 obtain micromorphological images of biogenic carbonate on microscope slides, (2 to quantify carbonate formation in fiberglass cloths, and (3 to measure associated carbon-isotope fractionations. The apparatuses were buried and harvested at monthly intervals from December 2010 to June 2011. The study was conducted along an ecological transect in New Mexico, USA, at three sites: a low-elevation desert (C3 shrubs, an intermediate-elevation steppe (C4 grasses, and a high-elevation forest (C3 conifers. In addition to comparing bioclimatic zones, the effect of parent material was also tested using paired limestone and igneous soils at each site. Microscope slides were analyzed with binocular, petrographic, and scanning electron microscopy equipped with an x-ray microanalyser (EDS, and the fiberglass traps were analyzed with x-ray diffraction and a mass spectrometer for carbon concentrations and isotope ratios. Naturally occurring calcified microbes were found at each site in the form of calcified hyphae, needle fiber, and calcified root hairs, with the exception of the forest site on igneous parent material. Liquid growth medium induced microbial calcification regardless of whether the vegetation was desert shrubs, grassland, or forest, and regardless of whether the parent material was igneous or limestone. Thus, the ability of soil microorganisms to biomineralize carbonate when supplied with liquid growth medium in situ is a phenomenon that crosses biomes and is not limited to microbes endemic to either limestone or igneous parent material.

  12. Spatial variations of community structures and methane cycling across a transect of Lei-Gong-Hou mud volcanoes in eastern Taiwan.

    Science.gov (United States)

    Wang, Pei-Ling; Chiu, Yi-Ping; Cheng, Ting-Wen; Chang, Yung-Hsin; Tu, Wei-Xain; Lin, Li-Hung

    2014-01-01

    This study analyzed cored sediments retrieved from sites distributed across a transect of the Lei-Gong-Hou mud volcanoes in eastern Taiwan to uncover the spatial distributions of biogeochemical processes and community assemblages involved in methane cycling. The profiles of methane concentration and carbon isotopic composition revealed various orders of the predominance of specific methane-related metabolisms along depth. At a site proximal to the bubbling pool, the methanogenic zone was sandwiched by the anaerobic methanotrophic zones. For two sites distributed toward the topographic depression, the methanogenic zone overlaid the anaerobic methanotrophic zone. The predominance of anaerobic methanotrophy at specific depth intervals is supported by the enhanced copy numbers of the ANME-2a 16S rRNA gene and coincides with high dissolved Fe/Mn concentrations and copy numbers of the Desulfuromonas/Pelobacter 16S rRNA gene. Assemblages of 16S rRNA and mcrA genes revealed that methanogenesis was mediated by Methanococcoides and Methanosarcina. pmoA genes and a few 16S rRNA genes related to aerobic methanotrophs were detected in limited numbers of subsurface samples. While dissolved Fe/Mn signifies the presence of anaerobic metabolisms near the surface, the correlations between geochemical characteristics and gene abundances, and the absence of aerobic methanotrophs in top sediments suggest that anaerobic methanotrophy is potentially dependent on iron/manganese reduction and dominates over aerobic methanotrophy for the removal of methane produced in situ or from a deep source. Near-surface methanogenesis contributes to the methane emissions from mud platform. The alternating arrangements of methanogenic and methanotrophic zones at different sites suggest that the interactions between mud deposition, evaporation, oxidation and fluid transport modulate the assemblages of microbial communities and methane cycling in different compartments of terrestrial mud volcanoes.

  13. Rabbit Achilles tendon full transection model – wound healing, adhesion formation and biomechanics at 3, 6 and 12 weeks post-surgery

    Science.gov (United States)

    Meier Bürgisser, Gabriella; Calcagni, Maurizio; Bachmann, Elias; Fessel, Gion; Snedeker, Jess G.; Giovanoli, Pietro

    2016-01-01

    ABSTRACT After tendon rupture repair, two main problems may occur: re-rupture and adhesion formation. Suitable non-murine animal models are needed to study the healing tendon in terms of biomechanical properties and extent of adhesion formation. In this study 24 New Zealand White rabbits received a full transection of the Achilles tendon 2 cm above the calcaneus, sutured with a 4-strand Becker suture. Post-surgical analysis was performed at 3, 6 and 12 weeks. In the 6-week group, animals received a cast either in a 180 deg stretched position during 6 weeks (adhesion provoking immobilization), or were re-casted with a 150 deg position after 3 weeks (adhesion inhibiting immobilization), while in the other groups (3 and 12 weeks) a 180 deg position cast was applied for 3 weeks. Adhesion extent was analyzed by histology and ultrasound. Histopathological scoring was performed according to a method by Stoll et al. (2011), and the main biomechanical properties were assessed. Histopathological scores increased as a function of time, but did not reach values of healthy tendons after 12 weeks (only around 15 out of 20 points). Adhesion provoking immobilization led to an adhesion extent of 82.7±9.7%, while adhesion inhibiting immobilization led to 31.9±9.8% after 6 weeks. Biomechanical properties increased over time, however, they did not reach full strength nor elastic modulus at 12 weeks post-operation. Furthermore, the rabbit Achilles tendon model can be modulated in terms of adhesion formation to the surrounding tissue. It clearly shows the different healing stages in terms of histopathology and offers a suitable model regarding biomechanics because it exhibits similar biomechanics as the human flexor tendons of the hand. PMID:27635037

  14. State-space approach to evaluate spatial variability of field measured soil water status along a line transect in a volcanic-vesuvian soil

    Directory of Open Access Journals (Sweden)

    A. Comegna

    2010-12-01

    Full Text Available Unsaturated hydraulic properties and their spatial variability today are analyzed in order to use properly mathematical models developed to simulate flow of the water and solute movement at the field-scale soils. Many studies have shown that observations of soil hydraulic properties should not be considered purely random, given that they possess a structure which may be described by means of stochastic processes. The techniques used for analyzing such a structure have essentially been based either on the theory of regionalized variables or to a lesser extent, on the analysis of time series. This work attempts to use the time-series approach mentioned above by means of a study of pressure head h and water content θ which characterize soil water status, in the space-time domain. The data of the analyses were recorded in the open field during a controlled drainage process, evaporation being prevented, along a 50 m transect in a volcanic Vesuvian soil. The isotropic hypothesis is empirical proved and then the autocorrelation ACF and the partial autocorrelation functions PACF were used to identify and estimate the ARMA(1,1 statistical model for the analyzed series and the AR(1 for the extracted signal. Relations with a state-space model are investigated, and a bivariate AR(1 model fitted. The simultaneous relations between θ and h are considered and estimated. The results are of value for sampling strategies and they should incite to a larger use of time and space series analysis.

  15. Rapid typing of Coxiella burnetii.

    Directory of Open Access Journals (Sweden)

    Heidie M Hornstra

    Full Text Available Coxiella burnetii has the potential to cause serious disease and is highly prevalent in the environment. Despite this, epidemiological data are sparse and isolate collections are typically small, rare, and difficult to share among laboratories as this pathogen is governed by select agent rules and fastidious to culture. With the advent of whole genome sequencing, some of this knowledge gap has been overcome by the development of genotyping schemes, however many of these methods are cumbersome and not readily transferable between institutions. As comparisons of the few existing collections can dramatically increase our knowledge of the evolution and phylogeography of the species, we aimed to facilitate such comparisons by extracting SNP signatures from past genotyping efforts and then incorporated these signatures into assays that quickly and easily define genotypes and phylogenetic groups. We found 91 polymorphisms (SNPs and indels among multispacer sequence typing (MST loci and designed 14 SNP-based assays that could be used to type samples based on previously established phylogenetic groups. These assays are rapid, inexpensive, real-time PCR assays whose results are unambiguous. Data from these assays allowed us to assign 43 previously untyped isolates to established genotypes and genomic groups. Furthermore, genotyping results based on assays from the signatures provided here are easily transferred between institutions, readily interpreted phylogenetically and simple to adapt to new genotyping technologies.

  16. Rapid purification of recombinant histones.

    Directory of Open Access Journals (Sweden)

    Henrike Klinker

    Full Text Available The development of methods to assemble nucleosomes from recombinant histones decades ago has transformed chromatin research. Nevertheless, nucleosome reconstitution remains time consuming to this day, not least because the four individual histones must be purified first. Here, we present a streamlined purification protocol of recombinant histones from bacteria. We termed this method "rapid histone purification" (RHP as it circumvents isolation of inclusion bodies and thereby cuts out the most time-consuming step of traditional purification protocols. Instead of inclusion body isolation, whole cell extracts are prepared under strongly denaturing conditions that directly solubilize inclusion bodies. By ion exchange chromatography, the histones are purified from the extracts. The protocol has been successfully applied to all four canonical Drosophila and human histones. RHP histones and histones that were purified from isolated inclusion bodies had similar purities. The different purification strategies also did not impact the quality of octamers reconstituted from these histones. We expect that the RHP protocol can be readily applied to the purification of canonical histones from other species as well as the numerous histone variants.

  17. Establishment of the inferior alveolar nerve transection combined experimental periodontitis animal modal in rats%大鼠下牙槽神经离断和牙周炎双重动物实验模型的建立

    Institute of Scientific and Technical Information of China (English)

    孙静; 李纾; 高艳; 张盼盼; 杨丕山

    2012-01-01

    PURPOSE: To establish an animal model of the inferior alveolar nerve (IAN) transected combined experimental periodontitis in rats, in order to provide a foundation for exploring the function of nerve factor in the occurrence and development of periodontitis and periodontal tissue regeneration in vivo. METHODS: Thirty-six adult SPF Wistar rats were divided into 3 groups randomly. The IAN-transection and periodontitis group (P1): steel-wire was used to ligate to the dental cervix of the dual first lower molars, the gingiva was lacerated using a dental probe, and the surgical transection of the IAN-transection was applied to the left side of the experimental rats, the animals were provided with high-carbonhydrate diet after surgery;the IAN-transection group (P2): the surgical transection of the IAN-transection was applied to the left side of the experimental group, the animals were fed on in routine way after surgery; the sham surgical group (N): no surgery was performed, the animals were fed on in routine way after surgery.6 weeks after the operation, specimens were collected by dissecting the operation areas after internal fixation and observed by clinical examination, X -ray, and histological examination. SPSS 13.0 software package were used for statistical analysis. RESULTS: The IAN-transection operation was successfully conducted in the study and there was no significant necrosis or ulceration observed in the nerve dissected rats. All experimental periodontitis models were established successfully at the time point; there was severe destruction of the periodontal tissues especially in the P1 group. There was bone losses in the specimens of IAN-transection side than that in non IAN-transection side according to X-ray examination 6 weeksafter operation both in the P1 and P2 group, especially in the P1 group. Periodontal tissues on both sides of the P2 group hadno obvious inflammation and periodontal destruction. CONCLUSIONS: The experimental periodontitis animal

  18. 78 FR 11678 - Notice of Inventory Completion: Grand Rapids Public Museum, Grand Rapids, MI

    Science.gov (United States)

    2013-02-19

    ... National Park Service Notice of Inventory Completion: Grand Rapids Public Museum, Grand Rapids, MI AGENCY: National Park Service, Interior. ACTION: Notice. SUMMARY: The Grand Rapids Public Museum has completed an... Grand Rapids Public Museum. Repatriation of the human remains and associated funerary objects to...

  19. 77 FR 11575 - Notice of Inventory Completion: Grand Rapids Public Museum, Grand Rapids, MI

    Science.gov (United States)

    2012-02-27

    ... National Park Service Notice of Inventory Completion: Grand Rapids Public Museum, Grand Rapids, MI AGENCY: National Park Service, Interior. ACTION: Notice. SUMMARY: The Grand Rapids Public Museum has completed an... Rapids Public Museum. Repatriation of the human remains and associated funerary objects to the...

  20. Neuronal injury external to the retina rapidly activates retinal glia, followed by elevation of markers for cell cycle re-entry and death in retinal ganglion cells.

    Directory of Open Access Journals (Sweden)

    Alba Galan

    Full Text Available Retinal ganglion cells (RGCs are neurons that relay visual signals from the retina to the brain. The RGC cell bodies reside in the retina and their fibers form the optic nerve. Full transection (axotomy of the optic nerve is an extra-retinal injury model of RGC degeneration. Optic nerve transection permits time-kinetic studies of neurodegenerative mechanisms in neurons and resident glia of the retina, the early events of which are reported here. One day after injury, and before atrophy of RGC cell bodies was apparent, glia had increased levels of phospho-Akt, phospho-S6, and phospho-ERK1/2; however, these signals were not detected in injured RGCs. Three days after injury there were increased levels of phospho-Rb and cyclin A proteins detected in RGCs, whereas these signals were not detected in glia. DNA hyperploidy was also detected in RGCs, indicative of cell cycle re-entry by these post-mitotic neurons. These events culminated in RGC death, which is delayed by pharmacological inhibition of the MAPK/ERK pathway. Our data show that a remote injury to RGC axons rapidly conveys a signal that activates retinal glia, followed by RGC cell cycle re-entry, DNA hyperploidy, and neuronal death that is delayed by preventing glial MAPK/ERK activation. These results demonstrate that complex and variable neuro-glia interactions regulate healthy and injured states in the adult mammalian retina.

  1. [Chemicohydrographic characteristics and the seasonal variations of nutrients at 35 degrees N transect in the cold water mass of the Southern Yellow Sea].

    Science.gov (United States)

    Wei, Qin-Sheng; Fu, Ming-Zhu; Ge, Ren-Feng; Zang, Jia-Ye

    2010-09-01

    Based on the four cruises during 2006-2007, the chemicohydrographic characteristics and the seasonal variations of nutrients at 35 degrees N transect in the cold water mass of the Southern Yellow Sea were analyzed. The results showed that: In Winter, hydrological conditions in the eastern part of the section was significantly influenced by the Yellow Sea Warm Current, and the vertical mixing in the deep water had not reached the bottom, which led to the different distribution of the elements in the eastern and western part of the section, the vertically uniform distribution was presented in the west and the upper water of the east, while the stratification structure was found in the bottom water of the east. In Spring, the Yellow Sea warm current residual water and the Qingdao cold water mass were the most obviously hydrological characteristics, and because of the increasing of the temperature in the upper water and the weakening of the vertical mixing, the stability of the water column increased gradually; compared with winter, the contour trend of DO, pH and nutrients in deep water transferred to the more horizontal direction, and with the arrival of spring blooming, the nutrient concentrations in the upper water decreased significantly, besides, the subsurface chlorophyll maximum (SCM) phenomena also began to emerge. In Summer, the section was influenced by the thermocline and the Yellow Sea cold water mass, which was accompanied with the maximum value phenomenon of DO and pH, in addition, the nutrient concentrations were low in upper water and high in bottom water, and compared with spring, the further reduction of the nutrient concentrations existed in upper water, but the nutrient concentrations were increasing dominantly in the water below the thermocline. In Autumn, with the decreasing of the temperature in the upper water and the enhancing of the vertical mixing, the stability of the water column decreased, but the vertical mixing in the deep water was

  2. Geochemistry driven trends in microbial diversity and function across a temperature transect of a shallow water hydrothermal system off Milos (Greece)

    Science.gov (United States)

    Bühring, Solveig I.; Amend, Jan P.; Gómez Sáez, Gonzalo V.; Häusler, Stefan; Hinrichs, Kai-Uwe; Pichler, Thomas; Pop Ristova, Petra; Price, Roy E.; Santi, Ioulia; Sollich, Miriam

    2014-05-01

    The shallow water hydrothermal vents off Milos Island, Greece, discharge hot, slightly acidic, reduced fluids into colder, slightly alkaline, oxygenated seawater. Gradients in temperature, pH, and geochemistry are established as the two fluids mix, leading to the formation of various microbial microniches. In contrast to deep-sea hydrothermal systems, the availability of sun light allows for a combination of photo- and chemotrophic carbon fixation. Despite the comparably easy accessibility of shallow water hydrothermal systems, little is known about their microbial diversity and functioning. We present data from a shallow hydrothermal system off Milos Island, one of the most hydrothermally active regions in the Mediterranean Sea. The physico-chemical changes from ambient seafloor to hydrothermal area were investigated and documented by in situ microsensor profiling of temperature, pH, total reduced sulfur and dissolved oxygen alongside porewater geochemistry. The spatial microbial diversity was determined using a combination of gene- and lipid-based approaches, whereas microbial functioning was assessed by stable isotope probing experiments targeting lipid biomarkers. In situ microprofiles indicated an extreme environment with steep gradients, offering a variety of microniches for metabolically diverse microbial communities. We sampled a transect along a hydrothermal patch, following an increase in sediment surface temperature from background to 90°C, including five sampling points up to 20 cm sediment depth. Investigation of the bacterial diversity using ARISA revealed differences in the community structure along the geochemical gradients, with the least similarity between the ambient and highly hydrothermally impacted sites. Furthermore, using multivariate statistical analyses it was shown that variations in the community structure could be attributed to differences in the sediment geochemistry and especially the sulfide content, and only indirectly to shifts in

  3. Patterns and driving factors of WUE and NUE in natural forest ecosystems along the North-South Transect of Eastern China

    Institute of Scientific and Technical Information of China (English)

    SHENG Wenping; REN Shujie; YU Guirui; FANG Huajun; JIANG Chunming; ZHANG Mi

    2011-01-01

    From July 2008 to August 2008,72 leaf samples from 22 species and 81 soil samples in the nine natural forest ecosystems were collected,from north to south along the North-South Transect of Eastern China (NSTEC).Based on these samples,we studied the geographical distribution patterns of vegetable water use efficiency (WUE) and nitrogen use efficiency (NUE),and analyzed their relationship with environmental factors.The vegetable WUE and NUE were calculated through the measurement of foliar δ 13C and C/N of predominant species,respectively.The results showed:(1) vegetable WUE,ranging from 2.13 to 28.67 mg C g-1 H2O,increased linearly from south to north in the representative forest ecosystems along the NSTEC,while vegetable NUE showed an opposite trend,increasing from north to south,ranging from 12.92 to 29.60 g C g-1 N.(2) Vegetable WUE and NUE were dominantly driven by climate and significantly affected by soil nutrient factors.Based on multiple stepwise regression analysis,mean annual temperature,soil phosphorus concentration,and soil nitrogen concentration were responding for 75.5% of the variations of WUE (p<0.001).While,mean annual precipitation and soil phosphorus concentration could explain 65.7% of the change in vegetable NUE (p<0.001).Moreover,vegetable WUE and NUE would also be seriously inf1uenced by atmospheric nitrogen deposition in nitrogen saturated ecosystems.(3)There was a significant trade-off relationship between vegetable WUE and NUE in the typical forest ecosystems along the NSTEC (p<0.001),indicating a balanced strategy for vegetation in resource utilization in natural forest ecosystems along the NSTEC.This study suggests that global change would impact the resource use efficiency of forest ecosystems.However,vegetation could adapt to those changes by increasing the use efficiency of shortage resource while decreasing the relatively ample one.But extreme impacts,such as heavy nitrogen deposition,would break this trade-off mechanism and

  4. Silicon limitation on primary production and its destiny in Jiaozhou Bay, China Ⅳ: Study on cross-bay transect from estuary to ocean

    Institute of Scientific and Technical Information of China (English)

    YANG Dongfang; CHEN Yu; GAO Zhenhui; ZHANG Jing; WANG Fan

    2005-01-01

    The authors analyzed the data collected in the Ecological Station Jiaozhou Bay from May 1991 to November 1994, including 12 seasonal investigations, to determine the characteristics, dynamic cycles and variation trends of the silicate in the bay. The results indicated that the rivers around Jiaozhou Bay provided abundant supply of silicate to the bay. The silicate concentration there depended on river flow variation. The horizontal variation of silicate concentration on the transect showed that the silicate concentration decreased with distance from shorelines. The vertical variation of it showed that silicate sank and deposited on the sea bottom by phytoplankton uptake and death, and zooplankton excretion. In this way, silicon would endlessly be transferred from terrestrial sources to the sea bottom. The silicon took up by phytoplankton and by other biogeochemical processes led to insufficient silicon supply for phytoplankton growth. In this paper, a 2D dynamic model of river flow versus silicate concentration was established by which silicate concentrations of 0.028-0.062 μmol/L in seawater was yielded by inputting certain seasonal unit river flows (m3/s), or in other words, the silicate supply rate; and when the unit river flow was set to zero, meaning no river input, the silicate concentrations were between 0.05 -0.69 μmol/L in the bay. In terms of the silicate supply rate, Jiaozhou Bay was divided into three parts. The division shows a given river flow could generate several different silicon levels in corresponding regions, so as to the silicon-limitation levels to the phytoplankton in these regions. Another dynamic model of river flow versus primary production was set up by which the phytoplankton primary production of 5.21-15.55(mgC/m2.d)/(m3/s) were obtained in our case at unit river flow values via silicate concentration or were achieved at zero unit river flow condition. A primary production conversion rate reflects the sensitivity to silicon depletion

  5. Effects of transection of cervical sympathetic trunk on cerebral infarct volume and oxygen free radical levels in rats with focal cerebral ischemia/reperfusion injury

    Institute of Scientific and Technical Information of China (English)

    Liangzhi Xiong; Yongxia Shi; Feng Xiao; Qingxiu Wang

    2008-01-01

    BACKGROUND: Stellate ganglion block (SGB) plays a protective role on the brain, but the precise mechanism of action is not clear.OBJECTIVE: To simulate SGB by transection of the cervical sympathetic trunk (TCST) and to investigate the TCST effects on changes in cerebral infarct volume and oxygen free radical levels in rats with focal cerebral ischemia/reperfusion injury.DESIGN, TIME AND SETTING: A complete randomized control animal experiment was performed at the Institute of Neurological Diseases of Taihe Hospital, Yunyang Medical College from February to December 2005.MATERIALS: A total of 101 healthy Wistar rats, weighing 280-320g, of both genders, aged 17-18 weeks, were used in this study. 2,3,5-triphenyltetrazolium chloride (TTC) was purchased from Changsha Hongyuan Biological Company. Superoxide dismutase (SOD), malondialdehyde (MDA) and nitric oxide (NO) assay kits were provided by Nanjing Jiancheng Bioengineering Institute.METHODS: Rats were randomly divided into a TCST group, a model group and a sham operation group. Successful models were included in the final analysis, with at least 20 rats in each group. After TCST, rat models of focal cerebral ischemia/reperfusion injury were established in the TCST group by receiving middle cerebral artery occlusion (MCAO) by the intraluminal suture method for 2 hours, followed by 24 hours of reperfusion. Rat models of focal cerebral ischemia/reperfusion injury were made in the model group. Rats in the sham operation group underwent experimental procedures as for the model group, threading depth of 10mm, and middle cerebral artery was not ligated.MAIN OUTCOME MEASURES: Brain tissue sections of ten rats from each group were used to measure cerebral infarct volume by TTC staining. Brain tissue homogenate of another ten rats from each group was used to detect SOD activities, MDA contents and NO levels. Rat neurological function was assessed by neurobehavioral measures.RESULTS: Cerebral infarct volume was bigger in the

  6. Rapid declines of large mammal populations after the collapse of the Soviet Union.

    Science.gov (United States)

    Bragina, Eugenia V; Ives, A R; Pidgeon, A M; Kuemmerle, T; Baskin, L M; Gubar, Y P; Piquer-Rodríguez, M; Keuler, N S; Petrosyan, V G; Radeloff, V C

    2015-06-01

    Anecdotal evidence suggests that socioeconomic shocks strongly affect wildlife populations, but quantitative evidence is sparse. The collapse of socialism in Russia in 1991 caused a major socioeconomic shock, including a sharp increase in poverty. We analyzed population trends of 8 large mammals in Russia from 1981 to 2010 (i.e., before and after the collapse). We hypothesized that the collapse would first cause population declines, primarily due to overexploitation, and then population increases due to adaptation of wildlife to new environments following the collapse. The long-term Database of the Russian Federal Agency of Game Mammal Monitoring, consisting of up to 50,000 transects that are monitored annually, provided an exceptional data set for investigating these population trends. Three species showed strong declines in population growth rates in the decade following the collapse, while grey wolf (Canis lupus) increased by more than 150%. After 2000 some trends reversed. For example, roe deer (Capreolus spp.) abundance in 2010 was the highest of any period in our study. Likely reasons for the population declines in the 1990s include poaching and the erosion of wildlife protection enforcement. The rapid increase of the grey wolf populations is likely due to the cessation of governmental population control. In general, the widespread declines in wildlife populations after the collapse of the Soviet Union highlight the magnitude of the effects that socioeconomic shocks can have on wildlife populations and the possible need for special conservation efforts during such times. PMID:25581070

  7. RAPID TRANSFER ALIGNMENT USING FEDERATED KALMAN FILTER

    Institute of Scientific and Technical Information of China (English)

    GUDong-qing; QINYong-yuan; PENGRong; LIXin

    2005-01-01

    The dimension number of the centralized Kalman filter (CKF) for the rapid transfer alignment (TA) is as high as 21 if the aircraft wing flexure motion is considered in the rapid TA. The 21-dimensional CKF brings the calculation burden on the computer and the difficulty to meet a high filtering updating rate desired by rapid TA. The federated Kalman filter (FKF) for the rapid TA is proposed to solve the dilemma. The structure and the algorithm of the FKF, which can perform parallel computation and has less calculation burden, are designed.The wing flexure motion is modeled, and then the 12-order velocity matching local filter and the 15-order attitud ematching local filter are devised. Simulation results show that the proposed EKE for the rapid TA almost has the same performance as the CKF. Thus the calculation burden of the proposed FKF for the rapid TA is markedly decreased.

  8. Rapid nuclear import of short nucleic acids.

    Science.gov (United States)

    Kitagawa, Mai; Okamoto, Akimitsu

    2016-10-01

    Exogenous short-chain nucleic acids undergo rapid import into the nucleus. Fluorescence-labeled dT1-13 DNA microinjected into the cytoplasm domain of a HeLa cell was rapidly imported into the nucleus domain within 1min. This is much more rapid than what has been observed for intracellular diffusion of small molecules. In contrast, import of longer nucleic acids with a length of over 30nt into the nucleus was suppressed. PMID:27597250

  9. Rapid Automated Mission Planning System Project

    Data.gov (United States)

    National Aeronautics and Space Administration — The proposed innovation is an automated UAS mission planning system that will rapidly identify emergency (contingency) landing sites, manage contingency routing,...

  10. Rapidity Correlation Structures from Causal Hydrodynamics

    CERN Document Server

    Gavin, Sean; Zin, Christopher

    2016-01-01

    Viscous diffusion can broaden the rapidity dependence of two-particle transverse momentum fluctuations. Surprisingly, measurements at RHIC by the STAR collaboration demonstrate that this broadening is accompanied by the appearance of unanticipated structure in the rapidity distribution of these fluctuations in the most central collisions. Although a first order classical Navier-Stokes theory can roughly explain the rapidity broadening, it cannot explain the additional structure. We propose that the rapidity structure can be explained using the second order causal Israel-Stewart hydrodynamics with stochastic noise.

  11. Rapid methods for detection of bacteria

    DEFF Research Database (Denmark)

    Corfitzen, Charlotte B.; Andersen, B.Ø.; Miller, M.;

    2006-01-01

    Traditional methods for detection of bacteria in drinking water e.g. Heterotrophic Plate Counts (HPC) or Most Probable Number (MNP) take 48-72 hours to give the result. New rapid methods for detection of bacteria are needed to protect the consumers against contaminations. Two rapid methods...

  12. Rapid eye movement sleep behavior disorder

    DEFF Research Database (Denmark)

    Schenck, C H; Montplaisir, J Y; Frauscher, B;

    2013-01-01

    We aimed to provide a consensus statement by the International Rapid Eye Movement Sleep Behavior Disorder Study Group (IRBD-SG) on devising controlled active treatment studies in rapid eye movement sleep behavior disorder (RBD) and devising studies of neuroprotection against Parkinson disease (PD...

  13. Set point calculations for RAPID project

    International Nuclear Information System (INIS)

    The Respond and Pump in Days (RAPID) project was initiated to pump part of the contents of tank 241-SY-101 into tank 241-SY-102. This document establishes the basis for all set points and ranges used in the RAPID project

  14. Set point calculations for RAPID project

    Energy Technology Data Exchange (ETDEWEB)

    HICKMAN, G.L.

    1999-10-18

    The Respond and Pump in Days (RAPID) project was initiated to pump part of the contents of tank 241-SY-101 into tank 241-SY-102. This document establishes the basis for all set points and ranges used in the RAPID project.

  15. Variability in pH, fCO 2, oxygen and flux of CO 2 in the surface water along a transect in the Atlantic sector of the Southern Ocean

    Science.gov (United States)

    Chierici, Melissa; Fransson, Agneta; Turner, David R.; Pakhomov, E. A.; Froneman, P. W.

    2004-11-01

    Underway sampling and measurements of pH, fCO 2, oxygen and Chlorophyll a (Chl a) were performed in the surface waters from Cape Town (South Africa) to Queen Maud Land (Antarctica) in the Atlantic sector of the Southern Ocean during the austral summer 1997/1998. From direct measurements of these parameters and from calculated fCO 2 the oceanic carbon dioxide system was studied and related to hydrological and biological parameters. fCO 2 was in general undersaturated relative to the atmosphere and showed a large variability with values ranging from 313 to 377 μatm with a mean value of 346±13 μatm. The undersaturation was more pronounced in areas associated with fronts where high Chl a and high pH in situ values were observed. Using shipboard wind speed data, estimates of the CO 2 flux were made along the transect and during three mesoscale surveys on the northward return transect in the area of the Spring Ice Edge (SIE), the Winter Ice Edge (WIE) and in the Antarctic Polar Front (APF). The undersaturation observed during the transect caused the ocean to act as a sink for CO 2 with a mean sea-air flux for the entire transect of -3±5 mmol m -2 d -1 with a large variability between -20 mmol m -2 d -1 (oceanic uptake) to 1.3 mmol m -2 d -1 (oceanic source). The lowest fCO 2 values (largest oceanic uptake of CO 2) were found at the southern boundary of the APF at 53°S, which coincided with a supersaturation in oxygen and high pH values. Oxygen concentrations were measured from 50°S to 63°S and varied between 324 and 359 μmol kg -1 with a mean value of 347±9 μmol kg -1. In general only small deviations from equilibrium oxygen saturation were observed (mean value=99±2%). However, in the SIE oxygen was clearly undersaturated, probably an effect of upwelling of oxygen poor deep water which had not yet been compensated for by biological production. Three weeks later, the ice edge had retreated in the SIE region and the Chl a concentration had increased three

  16. In vivo imaging of dorsal root regeneration: rapid immobilization and presynaptic differentiation at the CNS/PNS border.

    Science.gov (United States)

    Di Maio, Alessandro; Skuba, Andrew; Himes, B Timothy; Bhagat, Srishiti L; Hyun, Jung Keun; Tessler, Alan; Bishop, Derron; Son, Young-Jin

    2011-03-23

    Dorsal root (DR) axons regenerate in the PNS but turn around or stop at the dorsal root entry zone (DREZ), the entrance into the CNS. Earlier studies that relied on conventional tracing techniques or postmortem analyses attributed the regeneration failure to growth inhibitors and lack of intrinsic growth potential. Here, we report the first in vivo imaging study of DR regeneration. Fluorescently labeled, large-diameter DR axons in thy1-YFPH mice elongated through a DR crush site, but not a transection site, and grew along the root at >1.5 mm/d with little variability. Surprisingly, they rarely turned around at the DREZ upon encountering astrocytes, but penetrated deeper into the CNS territory, where they rapidly stalled and then remained completely immobile or stable, even after conditioning lesions that enhanced growth along the root. Stalled axon tips and adjacent shafts were intensely immunolabeled with synapse markers. Ultrastructural analysis targeted to the DREZ enriched with recently arrived axons additionally revealed abundant axonal profiles exhibiting presynaptic features such as synaptic vesicles aggregated at active zones, but not postsynaptic features. These data suggest that axons are neither repelled nor continuously inhibited at the DREZ by growth-inhibitory molecules but are rapidly stabilized as they invade the CNS territory of the DREZ, forming presynaptic terminal endings on non-neuronal cells. Our work introduces a new experimental paradigm to the investigation of DR regeneration and may help to induce significant regeneration after spinal root injuries.

  17. Use of dissolved inorganic carbon isotopes to track photosynthesis, respiration, and nitrification along a 56 mile transect in the Sacramento River and San Francisco Bay

    Science.gov (United States)

    Silva, S. R.; Kendall, C.; Peek, S.; Young, M. B.

    2013-12-01

    A decline in phytoplankton stocks in the San Francisco Bay and Delta is thought to contribute to the pelagic organism decline observed over the past two decades. One factor controlling phytoplankton growth rate is the availability of nutrients. Although there is an excess of nutrients in the Bay and Delta, the type and relative abundance of nutrients is critical to phytoplankton growth. To evaluate the response of phytoplankton to nutrient sources and to better understand phytoplankton dynamics downstream, we tested the hypothesis that the δ13C values of dissolved inorganic carbon (DIC) along with conventional water chemistry analyses will record events such as increased nitrification (related to the Sacramento River Wastewater Treatment Plant ammonium input) and algal blooms, and reflect the balance between photosynthesis and bacterial respiration. Multiple parameters affect [DIC] and its δ13C, including DIC sources, pH, and biological processes. Consumption of CO2 by phytoplankton during photosynthesis and by autotrophic bacteria during nitrification both result in increases in δ13C-DIC. However, photosynthesis and nitrification have very different relationships to chlorophyll and nutrient concentrations. The balance between heterotrophic bacterial respiration and photosynthesis should be reflected in trends in DIC, nutrient, and chlorophyll concentration, and δ13C-DIC. The δ13C of DIC should also be reflected in the δ13C of phytoplankton with approximately a 20 per mil fractionation. Significant deviation in the fractionation factor may indicate local variations in growth rate, nutrient availability, or speciation. Combined, these parameters should provide a gauge of the relative importance of the above mentioned processes. To test this hypothesis, we collected 19 water samples per cruise between July 2012 and July 2013 along a 56 mile transect between Rio Vista on the Sacramento River and San Francisco Bay near Angel Island during 8 cruises on the USGS RV

  18. A new method for rapid Canine retraction

    Directory of Open Access Journals (Sweden)

    "Khavari A

    2001-06-01

    Full Text Available Distraction osteogenesis method (Do in bone lengthening and rapid midpalatal expansion have shown the great ability of osteognic tissues for rapid bone formation under distraction force and special protocol with optimum rate of one millimeter per day. Periodontal membrane of teeth (PDM is the extension of periostium in the alveolar socked. Orthodontic force distracts PDM fibers in the tension side and then bone formation will begin.Objects: Rapid retraction of canine tooth into extraction space of first premolar by DO protocol in order to show the ability of the PDM in rapid bone formation. The other objective was reducing total orthodontic treatment time of extraction cases.Patients and Methods: Tweleve maxillary canines in six patients were retracted rapidly in three weeks by a custom-made tooth-born appliance. Radiographic records were taken to evaluate the effects of heavy applied force on canine and anchorage teeth.Results: Average retraction was 7.05 mm in three weeks (2.35 mm/week. Canines rotated distal- in by mean 3.5 degrees.Anchorage loss was from 0 to 0.8 mm with average of 0.3 mm.Root resorption of canines was negligible, and was not significant clinically. Periodontium was normal after rapid retraction. No hazard for pulp vitality was observed.Discussion: PDM responded well to heavy distraction force by Do protocol. Rapid canine retraction seems to be a safe method and can considerabely reduce orthodontic time.

  19. Risks and benefits of rapid clozapine titration

    Directory of Open Access Journals (Sweden)

    Jeannie D. Lochhead

    2016-05-01

    Full Text Available Clozapine is often considered the gold standard for the treatment of schizophrenia. Clinical guidelines suggest a gradual titration over 2 weeks to reduce the risks of adverse events such as seizures, hypotension, agranulocytosis, and myocarditis. The slow titration often delays time to therapeutic response. This raises the question of whether, in some patients, it may be safe to use a more rapid clozapine titration. The following case illustrates the potential risks associated with the use of multiple antipsychotics and rapid clozapine titration. We present the case of a young man with schizophrenia who developed life threatening neuroleptic malignant syndrome (NMS during rapid clozapine titration and treatment with multiple antipsychotics. We were unable to find another case in the literature of NMS associated with rapid clozapine titration. This case is meant to urge clinicians to carefully evaluate the risks and benefits of rapid clozapine titration, and to encourage researchers to further evaluate the safety of rapid clozapine titration. Rapid clozapine titration has implications for decreasing health care costs associated with prolonged hospitalizations, and decreasing the emotional suffering associated with uncontrolled symptoms of psychosis. Clozapine is considered the most effective antipsychotic available thus efforts should focus on developing strategies that would allow for safest and most efficient use of clozapine to encourage its utilization for treatment resistance schizophrenia.

  20. Hadron rapidity spectra within a hybrid model

    CERN Document Server

    Khvorostukhin, A S

    2016-01-01

    A 2-stage hybrid model is proposed that joins the fast initial state of interaction, described by the hadron string dynamics (HSD) model, to subsequent evolution of the expanding system at the second stage, treated within ideal hydrodynamics. The developed hybrid model is assigned to describe heavy-ion collisions in the energy range of the NICA collider under construction in Dubna. Generally, the model is in reasonable agreement with the available data on proton rapidity spectra. However, reproducing proton rapidity spectra, our hybrid model cannot describe the rapidity distributions of pions. The model should be improved by taking into consideration viscosity effects at the hydrodynamical stage of system evolution.

  1. The inventory of the terrestrial part of land-to-sea transects on Olkiluoto island in 2008 and the investigations of reedbeds surrounding Olkiluoto island carried out in 2007-2008

    International Nuclear Information System (INIS)

    In 2008, six transects running from land to sea were established on Olkiluoto Island, where soil and vegetation properties were studied at the transition zone and land- and seawards from the waterline. This Working Report presents results of the inventory on the land side. The soils varied from very fine grained to stony till soils and bedrock outcrops. This variation was reflected in the vegetation. In forested areas, the least diverse field layers were met in transect sections with sandy till soils or bedrock outcrops, where the canopies were already closed. Groves were situated on fine sand soils. The transition zone between sea and land differed from line to line and depended on soil properties, topography and degree of shelter. Human actions (e.g. various construction works) have greatly affected the land cover and soils. In addition to Olkiluoto, similar methodology was applied on shorelines of two so called reference lakes (Koskeljaervi and Lutanjaervi). Furthermore, this Working Report describes reedbed inventories carried out on Olkiluoto on 2007 and 2008. Reed was met on gyttja, clay, till and stone bottoms. The extent and vitality of the reedbeds varied relatively much, depending on the soil type and degree of shelter. In water, widest reedbeds were located by the bridge connecting the island to mainland. On the terrestrial side, competitive reed was met very far from the shoreline in a few large bays with clay soil. Summer cottages and dredging had affected the natural extent of reed. (orig.)

  2. Coral bleaching, hurricane damage, and benthic cover on coral reefs in St. John, U.S. Virgin Islands: A comparison of surveys with the chain transect method and videography

    Science.gov (United States)

    Rogers, C.S.; Miller, J.

    2001-01-01

    The linear chain transect method and videography were used to quantify the percent cover by corals, macroalgae, gorgonians, other living organisms, and substrate along permanent transects on two fringing reefs off St. John. Both methods were used simultaneously on Lameshur reef in November 1998, and on Newfound reef in March and October 1998. Hurricane Georges passed over St. John in September 1998, and a severe coral bleaching episode began the same month. Both methods gave remarkably similar values for coral cover, while the video method gave consistently higher values for gorgonians and macroalgae. The most dramatic difference was in the quantification of bleaching. At Newfound, the chain method indicated 13.4% (SD = 14.1) of the coral tissues were bleached and the video method, 43.4% (SD = 13.0). Corresponding values at Lameshur were 18.1% (SD = 22.3) and 46.5% (SD = 13.3). Although hurricane damage was conspicuous at Newfound reef, neither method showed significant changes in coral cover or other categories as a result of the storm.

  3. Zika Spreading Rapidly Through Puerto Rico: CDC

    Science.gov (United States)

    ... page: https://medlineplus.gov/news/fullstory_159430.html Zika Spreading Rapidly Through Puerto Rico: CDC Possibly hundreds ... 2016 FRIDAY, June 17, 2016 (HealthDay News) -- The Zika virus is spreading fast through Puerto Rico, placing ...

  4. Soft-/rapidity- anomalous dimensions correspondence

    CERN Document Server

    Vladimirov, Alexey A

    2016-01-01

    We establish a correspondence between ultraviolet singularities of soft factors for multi-particle production and rapidity singularities of soft factors for multi-parton scattering. This correspondence is a consequence of a conformal mapping between scattering geometries. The correspondence is valid to all orders of perturbation theory and in this way provides a proof of rapidity renormalization procedure for multi-parton scattering soft factors (including the transverse momentum dependent (TMD) soft factor as a special case). As a by-product we obtain an exact relation between the rapidity anomalous dimension and the well-known soft anomalous dimension. The three-loop rapidity anomalous dimensions for TMD and a general multi-parton scattering are derived.

  5. Ceramic microfabrication by rapid prototyping process chains

    Indian Academy of Sciences (India)

    R Knitter; W Bauer

    2003-02-01

    Fabrication of micropatterned ceramics or ceramic microparts make high demands on the precision and resolution of the moulding process. As finishing of miniaturised or micropatterned ceramic components is nearly impossible, shaping has to be done by a replication step in the green, unfired state. To avoid high tooling costs in product development, a rapid prototyping process chain has been established that enables rapid manufacturing of ceramic microcomponents from functional models to small lot series within a short time. This process chain combines the fast and inexpensive supply of master models by rapid prototyping with accurate and flexible ceramic manufacturing by low-pressure injection moulding. Besides proper feedstock preparation and sufficient small grain size, the quality of the final components is mainly influenced by the quality of the master model. Hence, the rapid prototyping method must be carefully selected to meet the requirements of the component to be fabricated.

  6. Rapid Update Cycle (RUC) [13 km

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Rapid Update Cycle (RUC) weather forecast model was developed by the National Centers for Environmental Prediction (NCEP). On May 1, 2012, the RUC was replaced...

  7. Development of a Rapid Thermoplastic Impregnation Device

    NARCIS (Netherlands)

    Weustink, A.P.D.

    2007-01-01

    A melt impregnation device for rapid thermoplastic impregnation of fiber bundles has been developed through modeling and experiments. The basic principles behind the thermoplastic impregnation process are investigated and the properties needed for a successful thermoplastic impregnation device are f

  8. Colorado Plateau Rapid Ecoregion Assessment Data Catalog

    Data.gov (United States)

    Bureau of Land Management, Department of the Interior — Datasets used in the analysis of the Colorado Plateau (COP) Rapid Ecoregion Assessment (REA).They can be downloaded via a layer package (lpk, similar to a zip file...

  9. Rapid Development of the Radiation Curing Sector

    Institute of Scientific and Technical Information of China (English)

    2007-01-01

    @@ Radiation curing is an advanced material surface treatment technology using ultraviolet (UV) radiation and electronic beams (EB). With the greater attention paid to environ mentel protection in recent years ,radiation curing has developed rapidly.

  10. Rapidity Correlation Structure in Nuclear Collisions

    CERN Document Server

    Gavin, Sean; Zin, Christopher

    2016-01-01

    We show that measurements of the rapidity dependence of transverse momentum correlations can be used to determine the characteristic time $\\tau_\\pi$ that dictates the rate of isotropization of the stress energy tensor, as well as the shear viscosity $\

  11. The Rapid Perceptual Impact of Emotional Distractors.

    Directory of Open Access Journals (Sweden)

    Briana L Kennedy

    Full Text Available The brief presentation of an emotional distractor can temporarily impair perception of a subsequent, rapidly presented target, an effect known as emotion-induced blindness (EIB. How rapidly does this impairment unfold? To probe this question, we examined EIB for targets that immediately succeeded ("lag-1" emotional distractors in a rapid stream of items relative to EIB for targets at later serial positions. Experiments 1 and 2 suggested that emotional distractors interfere with items presented very soon after them, with impaired target perception emerging as early as lag-1. Experiment 3 included an exploratory examination of individual differences, which suggested that EIB onsets more rapidly among participants scoring high in measures linked to negative affect.

  12. An Exponential Regulator for Rapidity Divergences

    CERN Document Server

    Li, Ye; Zhu, Hua Xing

    2016-01-01

    Finding an efficient and compelling regularization of soft and collinear degrees of freedom at the same invariant mass scale, but separated in rapidity is a persistent problem in high-energy factorization. In the course of a calculation, one encounters divergences unregulated by dimensional regularization, often called rapidity divergences. Once regulated, a general framework exists for their renormalization, the rapidity renormalization group (RRG), leading to fully resummed calculations of transverse momentum (to the jet axis) sensitive quantities. We examine how this regularization can be implemented via a multi-differential factorization of the soft-collinear phase-space, leading to an (in principle) alternative non-perturbative regularization of rapidity divergences. As an example, we examine the fully-differential factorization of a color singlet's momentum spectrum in a hadron-hadron collision at threshold. We show how this factorization acts as a mother theory to both traditional threshold and transve...

  13. Rapid Update Cycle (RUC) [20 km

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Rapid Update Cycle (RUC) weather forecast model was developed by the National Centers for Environmental Prediction (NCEP). On May 1, 2012, the RUC was replaced...

  14. A rapid method of ferromanganese nodule mounting

    Digital Repository Service at National Institute of Oceanography (India)

    Mukhopadhyay, S.; Banerjee, R.

    GEOLOGICAL SOCIETY OF INDIA Vol. 36. Aug. 1990. pp. 173 to 174 A Rapid Method of Ferromanganese Nodule Mounting S. MUKHOPADHYAYl AND R. BANERJEE2 1 Department of Geological Sciences, Jadavpur University, Calcutta 700032 2 National Institute of Oceanography.... Dona Paula, Goa 403 004 Abstract. A rapid method of sample preparation for ferromanganese nodules andcrusts mounting is presented here. by using laminated polyester resin. cobalt napthanate and methyl ethyl ketone peroxide. With- this technique vacuum...

  15. Rapid coal proximate analysis by thermogravimetric method

    Energy Technology Data Exchange (ETDEWEB)

    Mao Jianxiong; Yang Dezhong; Zhao Baozhong

    1987-09-01

    A rapid coal proximate analysis by thermogravimetric analysis (TGA) can be used as an alternative method for the standard proximate analysis. This paper presents a program set up to rapidly perform coal proximate analysis by using a thermal analyzer and TGA module. A comparison between coal proximate analyses by standard method (GB) and TGA is also given. It shows that most data from TGA fall within the tolerance limit of standard method.

  16. Rapid HIV Testing in Large Urban Jails

    OpenAIRE

    Beckwith, Curt G.; Nunn, Amy; Baucom, Sharon; Getachew, Asresahegn; Akinwumi, Akin; Herdman, Bruce; DiBartolo, Phil; Spencer, Susan; Brown, Devon; Lesansky, Henry; Kuo, Irene

    2012-01-01

    HIV prevalence is higher in jails than in the community, yet many jails do not conduct HIV testing. Jails in Baltimore, Maryland; Philadelphia, Pennsylvania; and the District of Columbia have implemented innovative rapid HIV testing programs. We have summarized the results of these programs, including the numbers of persons tested, rapid and confirmatory HIV test results, and numbers of persons newly diagnosed with HIV. We have described facilitators and challenges of implementation. These pr...

  17. Exploring data with RapidMiner

    CERN Document Server

    Chisholm, Andrew

    2013-01-01

    A step-by-step tutorial style using examples so that users of different levels will benefit from the facilities offered by RapidMiner.If you are a computer scientist or an engineer who has real data from which you want to extract value, this book is ideal for you. You will need to have at least a basic awareness of data mining techniques and some exposure to RapidMiner.

  18. Review on CNC-Rapid Prototyping

    Science.gov (United States)

    Z, M. Nafis O.; Y, Nafrizuan M.; A, Munira M.; J, Kartina

    2012-09-01

    This article reviewed developments of Computerized Numerical Control (CNC) technology in rapid prototyping process. Rapid prototyping (RP) can be classified into three major groups; subtractive, additive and virtual. CNC rapid prototyping is grouped under the subtractive category which involves material removal from the workpiece that is larger than the final part. Richard Wysk established the use of CNC machines for rapid prototyping using sets of 2½-D tool paths from various orientations about a rotary axis to machine parts without refixturing. Since then, there are few developments on this process mainly aimed to optimized the operation and increase the process capabilities to stand equal with common additive type of RP. These developments include the integration between machining and deposition process (hybrid RP), adoption of RP to the conventional machine and optimization of the CNC rapid prototyping process based on controlled parameters. The article ended by concluding that the CNC rapid prototyping research area has a vast space for improvement as in the conventional machining processes. Further developments and findings will enhance the usage of this method and minimize the limitation of current approach in building a prototype.

  19. Review on CNC-Rapid Prototyping

    International Nuclear Information System (INIS)

    This article reviewed developments of Computerized Numerical Control (CNC) technology in rapid prototyping process. Rapid prototyping (RP) can be classified into three major groups; subtractive, additive and virtual. CNC rapid prototyping is grouped under the subtractive category which involves material removal from the workpiece that is larger than the final part. Richard Wysk established the use of CNC machines for rapid prototyping using sets of 2½-D tool paths from various orientations about a rotary axis to machine parts without refixturing. Since then, there are few developments on this process mainly aimed to optimized the operation and increase the process capabilities to stand equal with common additive type of RP. These developments include the integration between machining and deposition process (hybrid RP), adoption of RP to the conventional machine and optimization of the CNC rapid prototyping process based on controlled parameters. The article ended by concluding that the CNC rapid prototyping research area has a vast space for improvement as in the conventional machining processes. Further developments and findings will enhance the usage of this method and minimize the limitation of current approach in building a prototype.

  20. Modified TB rapid test by proteinase K for rapid diagnosis of pleural tuberculosis.

    Science.gov (United States)

    Yari, Shamsi; Hadizadeh Tasbiti, Alireza; Ghanei, Mostafa; Shokrgozar, Mohammad Ali; Fateh, Abolfazl; Yari, Fatemeh; Bahrmand, Ahmadreza

    2016-03-01

    The diagnosis of pleural tuberculosis continues to be a challenge due to the low sensitivity of traditional diagnostic methods. Better and more rapid tests are needed for diagnosis of pleural TB. In this study, pleural fluids were tested with rapid test to determine Mycobacterium tuberculosis (MTB antigen). Affinity chromatography was used to purify specific polyclonal antibodies against MTB antigen. Pleural samples after decontamination were treated with proteinase K. Rapid test for pleural fluids was prepared by specific antibody. Rapid test was performed on 85 pleural fluid patients. The patients had a mean age of 46.55 ± 15.96 years and 38 were men. The performance of rapid test, using proteinase K, was found to be the most impressive: sensitivity 93%, specificity 94%, PPV 90%, and NPV 96% compared with adenosine deaminase test (ADA), PCR, smear, and culture. The present study did demonstrate that modified TB rapid test can substantially improve the diagnosis of extrapulmonary TB.