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Sample records for blood serum

  1. Blood serum atherogenicity and coronary artery calcification.

    Science.gov (United States)

    Sobenin, Igor A; Myasoedova, Veronica A; Anisimova, Elena V; Pavlova, Xenia N; Möhlenkamp, Stefan; Schmermund, Axel; Seibel, Rainer; Berenbein, Sina; Lehmann, Nils; Moebus, Susanne; Jöckel, KarlHeinz; Orekhov, Alexander N; Erbel, Raimund

    2014-01-01

    The phenomenon of blood serum atherogenicity was described as the ability of human serum to induce lipid accumulation in cultured cells. The results of recent two-year prospective study in asymptomatic men provided the evidence for association between the changes in serum atherogenicity and dynamics of carotid intima-media thickness progression. The present study was undertaken to test the hypothesis that blood serum atherogenicity and its changes in dynamics may be associated with accumulation of coronary calcium in subclinical atherosclerosis. It was performed in 782 CHD-free participants of The Heinz Nixdorf RECALL (Risk Factors, Evaluation of Coronary Calcium and Lifestyle) Study, in whom blood samples have been taken at the baseline and at the end of 5-year follow-up. Opposite to the previous findings, the changes in serum atherogenicity did not correlate neither with the extent of coronary artery calcification, nor with the changes in Agatston CAC score. There was a moderate but significant rise in serum atherogenicity after 5-year followup period, and the same dynamics was observed for Agatston CAC score, but not for convenient lipid-related risk factors. The absence of association of the changes in serum atherogenicity with the changes in Agatston CAC score, along with previous findings, provides a point of view that serum-induced intracellular cholesterol accumulation is not related to the processes of calcium deposition in arterial wall, since the last one reflects the progression of already existing subclinical atherosclerotic lesions. PMID:24533940

  2. Deuterium content variation of human blood serum

    International Nuclear Information System (INIS)

    We report, for the first time, the variation of deuterium content of blood serum of the patients with cancer disease. The tumorous cell, because of the higher growth speed , is deuterium consuming. The deuterium content of blood serum of cancerous human is diminished by about 5-7 ppm compared with that of the healthy individual. This effect is in accordance with some previous results. The replacement of tap water with deuterium depleted water in a drinking water of tumorous mice diminished the growth rate of tumors and the slight increase in the deuterium concentration stimulates growth because it is more easy for the cells to elevate the intracellular deuterium concentration up to the threshold level. (authors)

  3. The low TSH level in blood serum

    International Nuclear Information System (INIS)

    The low TSH level in blood serum (0.38 - 4,8 mμ/l) and the frequency of cross-reactions dictate the need for a sensitive and specific assay method. The IRMA (immunoradiometric assay) technique meets the requirements for high sensitivity and specificity. In the present study work was done to determine the characteristics of the IRMA-TSH (Netria-Batan) kit which makes use of the ''sandwich'' technique. Samples/standards were reacted with excess I-125 labelled monoclonal antibodies against TSH (Ab*), the free and the bound Ab* fraction were separated using solid phase antibody. The result showed that the IRMA-TSH (Netria-Batan) kit has abord working range (0.2 - 550 mμ/I) and a Low blank (Bo) value (0.8%-0.095%), high precision (with in assay CV less than 10%, and between assay CV less than 15%) high sensitivity (detection limit 0.04 mμ/l), and high accuracy (recovery 94,1%). High specificity of the kit was assured by the use of monoclonal anti-TSH with low cross reaction (0.008% against hCG, 0.7% against LH and 1.7% against FSH (1). (author). 7 figs.; 7 refs

  4. Radioimmunoassay of serum β2-microglobulin in donor's blood

    International Nuclear Information System (INIS)

    Serum β2-microglobulin (β2-MG) was tested by radioimmunoassay in 149 donors' and 54 healthy volunteers' blood. The results were 203 +- 33.0 nmol/l and 176 +- 26.2 nmol/l, respectively. There was significant difference statistically between them (P2-MG content. In order to increase the quality of donated blood and to keep the health of blood donor, it is suggested that the high content of serum β2-MG is the indicator of too frequent blood donating. The results also showed that the content of β2-MG in donor's blood is not a normal reference value

  5. Depressed serum erythropoietin in pregnant women with elevated blood lead.

    Science.gov (United States)

    Graziano, J H; Slavkovic, V; Factor-Litvak, P; Popovac, D; Ahmedi, X; Mehmeti, A

    1991-01-01

    During the course of a prospective study of lead exposure and pregnancy outcome in 1,502 women, we tested the hypothesis that environmental lead exposure is associated with depressed serum erythropoietin concentration. At mid-pregnancy and at delivery, blood samples were stratified by hemoglobin concentration; within each hemoglobin stratum, sera of women with the lowest and highest whole blood lead concentrations were selected for serum erythropoietin analysis. Analysis of variance revealed that women with higher blood lead levels had inappropriately low serum erythropoietin at both mid-pregnancy and at delivery. Thus, depressed serum erythropoietin appears to indicate lead nephrotoxicity, and it may also be responsible for the anemia associated with lead poisoning. PMID:1772259

  6. Association of serum uric acid with blood urea and serum creatinine

    International Nuclear Information System (INIS)

    Background: Hyperuricemia can cause serious health problems including renal insufficiency. Hyperuricemia is associated with many diseases including Hypertension, Diabetes Mellitus, Hypertriglyceridemia and Obesity. Objective of the present study was to study the Association of Serum Uric Acid with Blood Urea and Serum Creatinine. Methods: Eighty subjects, aged above 40, having blood urea more than 40 mg/dl and serum Creatinine more than 1.3 mg/dl were selected. 52.5 % subjects were male. Eighty subjects were selected as control group matching the age and sex with study group with normal blood urea and serum Creatinine. Results: Serum Uric Acid was found to be raised in 33 patients. Mean Serum Uric Acid value was 6.98+-2.021 in males (p<0.05) and 5.054+-2.324 in females (p<0.05). Conclusion: Serum Uric Acid is raised in patients with impaired renal function (p<0.05). Levels of increased Serum Uric Acid were not significantly associated with the cause of renal disease. (author)

  7. TRANSFERRIN: VARIATIONS IN BLOOD SERUM OF RED HOWLER MONKEYS.

    Science.gov (United States)

    SCHOEN, M A; ARENDS, T

    1964-11-01

    Blood serum samples from 33 red howler monkeys (Alouatta seniculus) were examined. Three different phenotypes were found and denominated A, B, and C. Four serums could not be classified because their transferrin apparently did not bind iron-59, possibly owing to saturation. A difference was observed in the electrophoretic migration and pattern of the transferrins in these monkeys compared with those of other primates. PMID:14197564

  8. Reagents for Lithium Electrodes and Sensors for Blood Serum Analysis

    Directory of Open Access Journals (Sweden)

    Gary D. Christian

    2002-10-01

    Full Text Available The measurement of lithium in blood serum requires high selectivity since the blood contains about 140 mM sodium compared to the 0.5-1.5 mM lithium level in manic depressive patients under treatment with lithium salts. This review traces the development of optical and potentiometric methods for the selective measurement of lithium in the presence of sodium. Selectivities of over 1,000:1 are achievable with properly designed ionophores.

  9. Enzyme immunoassay of immunoreactive trypsin in serum and blood spots

    International Nuclear Information System (INIS)

    An enzyme immunoassay method for the assay of serum immunoreactive trypsin (IRT) is described. The method is a two site binding assay carried out on microtitre plates as the solid phase. Wells were coated with affinity purified anti-human trypsin and bioinylated anti-trypsin and avidin-β-galactosidase were used as the second antibody and detection system respectively. The assay was sensitive enough to determine IRT concentrations in either serum or dried blood spots. A good correlation was obtained when the method was compared with the Hoechst radioimmunoassay method. (Author)

  10. Changes of blood serum in acute cerebral infarction after treatment

    International Nuclear Information System (INIS)

    Objective: To investigate the changes of serum of HDL, LDL, TXB2, 6-K-PGF1α, TG and β-LP in acute cerebral infarction after treatment. Method: The serum levels of HDL, LDL, TXB2, 6-K-PGF1α, TG and β-LP in 94 acute cerebral infarction patients were detected by RIA and biochemistry assay respectively. Results: The results showed that the serum HDL and 6-K-PGF1α levels in acute cerebral infarction patients were increased (P2 were decreased (P<0.01) after treatment. Conclusion: The Hyper coagulable state and blood fat index in acute cerebral infarction after treatment could improve obviously. (authors)

  11. Blood serum components and serum protein test of Hybro-PG broilers of different ages

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    PRL Silva

    2007-12-01

    Full Text Available Blood serum samples of HYBRO PG broilers were analyzed, with 30 samples collected from 21-day-old broilers (G1, 30 from 35-day-old birds (G2, and 30 from 42-day-old birds (G3, with the aim of establishing normal values of some blood serum parameters. The activities of the enzymes gamma-glutamyl-transferase (GGT, aspartate aminotransferase (AST, creatine kinase (CK, alkaline phosphatase (ALP, and lactate dehydrogenase (LDH, serum levels of total calcium, calcium ion, phosphorus, sodium, potassium, magnesium, chlorides, creatinine, uric acid, triglycerides, cholesterol, total protein, albumin, total and indirect and direct bilirubin, and electrophoretic profile of serum proteins in acrylamide (SDS-PAGE and agarose gel were determined. There was no influence of age on total bilirubin and albumin levels. All the other evaluated parameters presented differences in at least one age group. Protein electrophoretic profile also changed as a function of age. The obtained results can be considered as normal for the studied ages, and therefore be used as references for the interpretation of laboratory exams of broilers of this genetic line in the evaluated ages.

  12. Determination of phosphodiesterase I activity in human blood serum.

    Science.gov (United States)

    Hynie, I; Meuffels, M; Poznanski, W J

    1975-09-01

    Phosphodiesterase I (EC 3.1.4.1) activity was detected in normal human blood serum. The enzyme is stable at laboratory temperature for three days, but is inactivated at pH less than 7. The pH for optimum activity increases with the substrate concentration (under the conditions used, from pH 9.0 to 10.2) and, conversely, the Km increases with pH and buffer concentration. The enzyme is inhibited by ethylenediaminetetraacetate but not by phosphate (0.1 mol/liter). We developed a simple quantitative method for its determination, based on hydrolysis of the p-nitrophenyl ester of thymidine 5'-monophosphate and subsequent measurement of the liberated p-nitrophenol at 400 nm in NaOH (0.1 mol/liter). Normal values (mean +/- 2 SD) were determined to be 33 +/- 6.4 U/liter. Preliminary studies indicate that phosphodiesterase I activity is greater than normal in serum of patients with necrotic changes in the liver or kidney or in cases of breast cancer, but not in that of patients with myocardial infarction, bone cancer, lung cancer, or chronic liver cirrhosis. PMID:168991

  13. STUDY OF ERYTHROCYTE SEDIMENTATION RATE,SERUM TOTAL PROTEIN, SERUM ALBUMIN,SERUM GLOBULIN AND RED BLOOD CELL COUNT IN DIFFERENT PHASES OF MENSTRUAL CYCLE

    OpenAIRE

    Shilpa,; Girish M; Patil, MR; Malipatil

    2014-01-01

    : BACKGROUND: Menstruation is a phenomenon unique to females and nearly universal experience in women's lives and is poorly understood. It is characterized by co-ordinate sequence of hormonal changes but the changes that occur in the distribution of hematological parameters, biochemical parameters have not been clearly established. AIMS: To compare Erythrocyte Sedimentation Rate (ESR), Serum total protein, Serum albumin, Serum globulin and Red Blood Cell count (RBC) in dif...

  14. Ratio of blood serum ferritin and ferritin-bearing lymphocytes in breast cancer

    International Nuclear Information System (INIS)

    The ratio of the blood serum ferritin level and the amount of ferritin-bearing lymphocytes was determined in breast cancer patients and mastopathy patients. Direct relation between a rise of blood serum ferritin concentration, a drop in the amount of ferritin-bearing cells and tumor spreading was established in breast cancer. The maximum amount of ferritin-bearing cells was present in the blood of the patients with Stage I-IIA disease. Radical mastectomy in such patients returned the blood serum ferritin level and the amount of ferritin-bearing cells to normal

  15. STUDY OF ERYTHROCYTE SEDIMENTATION RATE,SERUM TOTAL PROTEIN, SERUM ALBUMIN,SERUM GLOBULIN AND RED BLOOD CELL COUNT IN DIFFERENT PHASES OF MENSTRUAL CYCLE

    Directory of Open Access Journals (Sweden)

    Shilpa

    2014-09-01

    Full Text Available : BACKGROUND: Menstruation is a phenomenon unique to females and nearly universal experience in women's lives and is poorly understood. It is characterized by co-ordinate sequence of hormonal changes but the changes that occur in the distribution of hematological parameters, biochemical parameters have not been clearly established. AIMS: To compare Erythrocyte Sedimentation Rate (ESR, Serum total protein, Serum albumin, Serum globulin and Red Blood Cell count (RBC in different phases of menstrual cycle. MATERIALS & METHODS: The present study was carried out on 100 healthy female medical students in the age group of 18 to 23years with normal menstrual cycle. E.S.R, Serum total protein, Serum albumin, Serum globulin and RBC count were studied on 2nd day MP(Menstrual Phase,11th day PP(Proliferative Phase, 22ndday SP(Secretory Phase of menstrual cycle using Wintergreen’s method, semi auto analyzer Erba chem-7(BCG Dye method for serum proteins, Hemo Auto analyzer SYSMEX KX-21 respectively. Statistical analysis was done using SPSS 17.0 Software. To compare means of two independent groups, student’s t- test for independent samples was used. RESULTS: The study revealed that ESR was significantly (P<0.01 higher in menstrual phase compared to proliferative phase. Serum total protein was significantly increased in Secretory Phase (SP compared to Menstrual Phase (MP (P<0.05. Serum albumin was significantly (P<0.05 increased in proliferative phase compared to secretory phase and menstrual phase. Serum globulin was significantly increased in SP compared to PP (P<0.05. Red blood cell count showed no significant changes during various phases of menstrual cycle. CONCLUSION: In the present study we found significantly low level of serum albumin and decreased RBC count though not significant increases rouleaux formation thus increasing ESR in the menstrual phase but serum globulin and serum total protein showed increase in secretory phase which does not

  16. Changing optical properties of blood serum proteins in case of oncological diseases

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    Petrova, G. P.; Petrusevich, Yu. M.; Boiko, A. V.; Ivanov, A. V.; Papish, E. A.; Khlapov, V. P.; Fedorova, K. V.

    2007-03-01

    Molecular methods of diagnostics of widespread diseases including oncological pathology on the base static and dynamic laser light scattering in serum blood solution are testified. Rayleigh - Debye laser light scattering method are used to measure molecular parameters of blood serum. Dynamical parameters of macromolecules can be measured by photon correlation spectroscopy method. We obtained that the parameter of intermolecular interaction B for serum blood solution of oncological patients is considerably less then B for serum blood solution of healthy persons and in a number of cases has even a negative value. The effective mass of scattering particles in serum water solutions for samples of oncology diseases patients increase in comparison to control samples. As follows from our experimental results, there is the difference between dynamic molecular parameters for control samples and ones with oncological pathology.

  17. Study on umbilical venous blood serum leptin and plasma neuropeptide Y levels in full term newborns

    International Nuclear Information System (INIS)

    Objective: To investigate the levels and clinical significance of umbilical venous blood serum leptin and plasma neuropeptide Y(NPY) in full term newborns. Methods: Umbilical venous blood serum leptin and plasma NPY levels were measured with radioimmunoassay. Results: The mean level of umbilical venous blood serum leptin was 7.15 +- 3.72 μg/L, the mean level of plasma NPY was 112.07 +- 42.36 ng/L. Levels in female fetal cord blood were significantly higher than those in males (p < 0.01). There were significantly positive correlations between cord venous blood serum leptin and birth weight of newborn and maternal body mass index (BMI) before delivery (r = 0.647, r = 0.547, p < 0.05); the same was true for plasma NPY ( r = 0.584, r = 0.705. p < 0.05). Conclusion: Leptin and NPY produced from fetus and placenta may regulate the fetal weight growth

  18. Effects of blood sample handling procedures on measurable inflammatory markers in plasma, serum and dried blood spot samples

    DEFF Research Database (Denmark)

    Skogstrand, K.; Thorsen, P.; Vogel, I.;

    2008-01-01

    whole blood samples at low temperatures and rapid isolation of plasma and serum. Effects of different handling procedures for all markers studied are given. DBSS proved to be a robust and convenient way to handle samples for immunoassay analysis of inflammatory markers in whole blood Udgivelsesdato......The interests in monitoring inflammation by immunoassay determination of blood inflammatory markers call for information on the stability of these markers in relation to the handling of blood samples. The increasing use of stored biobank samples for such ventures that may have been collected and...... stored for other purposes, justifies the study hereof. Blood samples were stored for 0, 4, 24, and 48 h at 4 degrees C, room temperature (RT), and at 35 degrees C, respectively, before they were separated into serum or plasma and frozen. Dried blood spot samples (DBSS) were stored for 0, 1, 2, 3, 7, and...

  19. [Properties of blood serum from reindeer and results of using it in virology and biotechnology].

    Science.gov (United States)

    Gunenkov, V V; Sukharev, O I; Sirota, V F

    2003-01-01

    The blood serum of the reindeer does not virtually contain any antibodies to respiratory and intestinal viruses widely spread among the cattle. The reindeer blood serum is comparable with the bovine serum by the contents of chlorides, hemoglobin, total protein and protein fractions; however, it is different from the latter by a 2.5-fold higher content of glucose and by a higher osmotic pressure. The serum stimulates the mitotic cell activity and cell monolayer production; it also contributes to accumulating the cytopathogenic viruses in cell culture. 5- and 10%-serum, when added to a nutrient medium, ensures the reproduction of bovine leucosis virus and the accumulation of the antigen of this virus in a continuous cell culture that is chronically infected with the virus. Finally, the reindeer serum stabilizes the viability of ovocytes and stimulates the bovine embryo development at transplantation. PMID:14708230

  20. The effects of coffee on serum lipids and blood pressure in a UK population.

    OpenAIRE

    Lancaster, T; Muir, J; Silagy, C

    1994-01-01

    Data on coffee consumption were collected for 1074 adults attending for health checks in the OXCHECK study, to assess associations with serum lipids and blood pressure. Of the sample, 70.5% drank coffee, largely instant. Coffee had no significant effects on total or high-density lipoprotein (HDL) cholesterol or blood pressure, and was negatively correlated with serum triglycerides. The type of coffee drunk in the UK does not adversely effect these cardiovascular risk factors.

  1. Estimation of free copper ion concentrations in blood serum using T1 relaxation rates

    Science.gov (United States)

    Blicharska, Barbara; Witek, Magdalena; Fornal, Maria; MacKay, Alex L.

    2008-09-01

    The water proton relaxation rate constant R1 = 1/ T1 (at 60 MHz) of blood serum is substantially increased by the presence of free Cu 2+ ions at concentrations above normal physiological levels. Addition of chelating agents to serum containing paramagnetic Cu 2+ nulls this effect. This was demonstrated by looking at the effect of adding a chelating agent—D-penicillamine (D-PEN) to CuSO 4 and CuCl 2 aqueous solutions as well as to rabbit blood serum. We propose that the measurement of water proton spin-lattice relaxation rate constants before and after chelation may be used as an alternative approach for monitoring the presence of free copper ions in blood serum. This method may be used in the diagnosis of some diseases (leukaemia, liver diseases and particularly Wilson's disease) because, in contrast to conventional methods like spectrophotometry which records the total number of both bound and free ions, the proton relaxation technique is sensitive solely to free paramagnetic ions dissolved in blood serum. The change in R1 upon chelation was found to be less than 0.06 s -1 for serum from healthy subjects but greater than 0.06 s -1 for serum from untreated Wilson's patients.

  2. ELISA with double antigen sandwich for screening specific serum anti-TP antibody in blood donors

    International Nuclear Information System (INIS)

    Objective: To select a sensitive and specific laboratory examination suitable for screening serum anti-TP antibody in blood donors. Methods: The serum anti-TP antibody in 11271 blood donors were detected using ELISA with double antigen sandwich and the outcomes were compared with those using RPR assay. The conflicting specimen were confirmed by repeating the test with TPHA assay. Results: The positive rates of serum anti-TP antibody by ELISA with double antigen sandwich and RPR was 0.36% (41/11271) and 0.26% (29/11271), respectively. The coincidence of the detecting outcomes by ELISA with double antigen sandwich and RPR with TPHA was 97.5% (40/41) and 63.41%(26/41) respectively. Conclusion: Compared with RPR assay, ELISA with double antigen sandwich has higher sensibility and specificity for screening serum anti-TP antibody in blood donors

  3. A Survey On Ionic And Metabolite Factors Of Blood Serum In Kutum (Rutilus frisii kutum

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    Afkhami Majid

    2014-10-01

    Full Text Available In this study, ionic parameters and metabolite factors (cholesterol, total protein, and glucose of serum and their interrelationships were detected in 48 specimens of kutum (Rutilus frisii kutum captured during spawning migration. Blood sampling was conducted by cutting the caudal peduncle of each sample, and blood was collected into heparinized and sterile capillary glass tubes.

  4. Optical diagnosis of dengue virus infection in human blood serum using Raman spectroscopy

    International Nuclear Information System (INIS)

    We present the optical diagnosis of dengue virus infection in human blood serum using Raman spectroscopy. Raman spectra were acquired from 18 blood serum samples using a laser at 532 nm as the excitation source. A multivariate regression model based on partial least-squares regression is developed that uses Raman spectra to predict dengue infection with leave-one-sample-out cross validation. The prediction of dengue infection by our model yields correlation coefficient r2 values of 0.9998 between the predicted and reference clinical results. The model was tested for six unknown human blood sera and found to be 100% accurate in accordance with the clinical results. (letter)

  5. Effect of a serum factor on IgE-mediated histamine release from whole blood.

    OpenAIRE

    Tanizaki,Yoshiro; Komagoe,Haruki; Sudo,Michiyasu; Morinaga, Hiroshi; Nakagawa,Saburo; Kitani,Hikaru; Takahashi, Kiyoshi; Kimura,Ikuro

    1984-01-01

    IgE-mediated histamine release from whole blood was analyzed in 44 patients with bronchial asthma by observing maximum present release and dose-response curves of histamine release induced by anti-IgE and house dust extract. The maximum histamine release from whole blood induced by anti-IgE correlated with total serum IgE levels. There was a close correlation between allergen-induced release from whole blood and the serum levels of specific IgE antibodies. In the maximum histamine release fro...

  6. Detecting free hemoglobin in blood plasma and serum with luminescent terbium complexes

    OpenAIRE

    Morgner, F.; Lecointre, A.; Charbonniere, L.J.; Lohmannsroben, H.G.

    2015-01-01

    Hemolysis, the rupturing of red blood cells, can result from numerous medical conditions (in vivo) or occur after collecting blood specimen or extracting plasma and serum out of whole blood (in vitro). In clinical laboratory practice, hemolysis can be a serious problem due to its potential to bias detection of various analytes or biomarkers. Here we present the first "mix-and-measure'' method to assess the degree of hemolysis in biosamples using luminescence spectroscopy. Luminescent terbium ...

  7. Auto-Immune Hemolytic Anemia in Patient who his Serum React with all ABO Blood Group

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    A Pourazar

    2004-12-01

    Full Text Available There are several irregular antibodies associated with various blood group systems which may cause some problems during blood cross matching in transfusion. The atypical antibodies are included auto and alloantibodies such as anti-I, anti-HI, anti-P… . In order to detect these antibodies, generally the agglutination reaction technique and anti-human-globulin (coombs tests would be performed and a panel of identified red blood cells will use if necessary for further investigation. During our work, we encountered with one serum sample that showed agglutination reaction with all the blood groups (A, B, O, and AB. We tested pooled red blood cells with OI group of adult and pooled cord red blood cells of Oi group with the patient serum. it was shown that the serum was reactive with OI but not with Oi. For confirmation of the result, the sample was sent to Institute of Immunohematology (I.I.H., India. The report approved that the serum contained anti-I specificity. To solve the transfusion problem for this patient, the recommendation is using the blood group with minimum coombs titration if the patient life is in threatened. Further investigations disclosed that the patient had leukemia. Keywords: Anti-I, Ii antigen, Allo-Autoantibody.

  8. Serum leptin levels correlation with high blood pressure in adult females

    International Nuclear Information System (INIS)

    To measure serum leptin levels and compare them in lean and obese subjects and to identify correlation between serum leptin levels, heart rate and hypertension in lean and obese subjects among adult females. Seventy female subjects with different body mass indices were selected from OPD of Jinnah Medical and Dental College Hospital (OPD), Karachi. Heart rate was counted manually; blood pressure was measured by mercury sphygmomanometer while serum leptin was measured using enzyme-linked immunoassay. The outcomes hypertension and heart rate were correlated to risk factor leptin. Mean heart rate, systolic and diastolic blood pressure and serum leptin levels of obese people were 90+-1, 142+-2, 89+-1 and 24.13+-1.7 respectively, which were significantly higher as compared to lean subjects (p<0.05). All the parameters correlated positively and significantly with increasing BMI. There was a relationship of tachycardia and hypertension with high serum leptin levels in obesity. Serum leptin levels increase with the level of obesity. Hyper-leptinemia is associated with tachycardia and increases in both systolic and diastolic blood pressure in obesity via complex mechanisms. (author)

  9. Chromium and cobalt ion concentrations in blood and serum following various types of metal-on-metal hip arthroplasties

    DEFF Research Database (Denmark)

    Jantzen, Christopher; Jørgensen, Henrik L; Duus, Benn R;

    2013-01-01

    Widely different metal ion concentrations in blood and serum have been reported with metal-on-metal (MoM) implants. We reviewed the literature on blood and serum ion concentrations of chromium (Cr) and cobalt (Co) following various MoM hip arthroplasties.......Widely different metal ion concentrations in blood and serum have been reported with metal-on-metal (MoM) implants. We reviewed the literature on blood and serum ion concentrations of chromium (Cr) and cobalt (Co) following various MoM hip arthroplasties....

  10. Radioimmunological determination of parathyroid normal concentration in the blood serum

    International Nuclear Information System (INIS)

    The radioimmunological method was used for determining the concentration of parathyroid hormone (PTH) in the serum. A comparative study was made of its two modifications with the use of the heterological systems ''antigen - antibody''. The hormone content of parathyroid glands was studied in practically healthy adults and in children at an age of 9-10 years. It was shown that the level of PTH in adults was 0.46+-0,04 ng/ml (in the set of the firm CIS) and 0.32+-0.18 ng/ml (in the set of the firm DRG). The concentration of PTH in children of the group studied was 1.00+-0.11 ng/ml (in the French set). Reproducibility of the method was studied

  11. Carbon dot based non enzymatic approach for the detection and estimation of glucose in blood serum

    Science.gov (United States)

    Shanti Krishna, A.; Nair, Priya A.; Radhakumary, C.; Sreenivasan, K.

    2016-05-01

    In this study we generated a simple, reliable and selective approach based on carbon dots (CDs) and 4-cyanophenylboronic acid (CPBA) for blood glucose sensing. The methodology relies on the quenching of the emission of CDs by CPBA followed by its recovery by glucose. The system consisting of CDs and CPBA was characterised by Fourier transform infra red spectrum, transmissions electron microscopic, dynamic light scattering instrument, UV–visible and fluorescence techniques. The response of the probe, CD-BA in presence of different concentrations of glucose was assessed. Linear range was obtained for glucose concentrations ranging from 1 to 30 mM. Interferences by other saccharides and various biomolecules coexisting in blood serum were negligible. The chemo sensor thus developed has been successfully used for the estimation of glucose in human blood serum. The system being sensitive, efficient and easy to perform is a promising platform for blood glucose sensing.

  12. Simultaneous determination of eight anticoagulant rodenticides in blood serum and liver.

    Science.gov (United States)

    Chalermchaikit, T; Felice, L J; Murphy, M J

    1993-01-01

    A liquid chromatographic method was developed for the analysis of indandione and 4-hydroxycoumarin anticoagulant rodenticides in blood serum and liver. The method enabled the measurement of serum and liver concentrations of eight anticoagulant rodenticides: brodifacoum, bromadiolone, chlorophacinone, coumafuryl, coumatetralyl, diphacinone, difenacoum, and warfarin. Anticoagulants were extracted from serum and liver with acetonitrile. Extracts were applied to solid-phase extraction columns, which contained mixed packings. Column eluates were evaporated to dryness, reconstituted, and subjected to reversed-phase liquid chromatography. Hydroxycoumarins were detected by fluorescence at an excitation wavelength of 318 nm and an emission wavelength of 390 nm. Indandiones were detected by UV absorption at 285 nm. Extraction efficiencies of greater than 75% for serum and greater than 69% for liver were obtained. The within-run precision (CV) ranged from 2.4 to 8.6% for serum and 2.6 to 8.7% for liver. The between-run precision (CV) ranged from 1.5 to 12.2% for serum and from 2.1 to 11.8% for liver. Hydroxycoumarin rodenticides were detected at 1 ng/mL of serum and 1 ng/g of liver. Indandiones were detected at 10 ng/mL of serum and 10 ng/g of liver. PMID:8429630

  13. Serum contents of endocannabinoids are correlated with blood pressure in depressed women

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    Ho WS Vanessa

    2012-02-01

    Full Text Available Abstract Background Depression is known to be a risk factor for cardiovascular diseases but the underlying mechanisms remain unclear. Since recent preclinical evidence suggests that endogenous agonists of cannabinoid receptors (endocannabinoids are involved in both cardiovascular function and depression, we asked whether endocannabinoids correlated with either in humans. Results Resting blood pressure and serum content of endocannabinoids in ambulatory, medication-free, female volunteers with depression (n = 28 and their age- and ethnicity-matched controls (n = 27 were measured. In females with depression, both diastolic and mean arterial blood pressures were positively correlated with serum contents of the endocannabinoids, N-arachidonylethanolamine (anandamide and 2-arachidonoylglycerol. There was no correlation between blood pressure and endocannabinoids in control subjects. Furthermore, depressed women had significantly higher systolic blood pressure than control subjects. A larger body mass index was also found in depressed women, however, it was not significantly correlated with serum endocannabinoid contents. Conclusions This preliminary study raises the possibility that endocannabinoids play a role in blood pressure regulation in depressives with higher blood pressure, and suggests an interrelationship among endocannabinoids, depression and cardiovascular risk factors in women.

  14. Determination of selenium in blood serum by inductively coupled plasma atomic emission spectrometry with pneumatic nebulization

    Energy Technology Data Exchange (ETDEWEB)

    Machat, Jiri; Kanicky, Viktor; Otruba, Vitezslav [Laboratory of Plasma Sources for Chemical Analysis-Laboratory of Atomic Spectrochemistry, Faculty of Science, Masaryk University Brno (Czech Republic)

    2002-02-01

    The possibility of determining selenium in blood serum using inductively coupled plasma emission spectrometry with conventional pneumatic nebulization was studied. A high-resolution spectrometer (SBW=6 pm) with laterally viewed ICP was employed. Analysis with conventional pneumatic nebulization could overcome laborious and demanding digestion, which is necessary for hydride generation. A pressure digestion with nitric acid at 160 C was sufficient to decrease the carbon content in the serum sample to 5%-10% of its original value. Spectral interference of the CN band was observed and mathematically corrected. It was found that the carbon-induced selenium line emission enhancement occurred even under ICP optimized conditions. A method of determination was developed and applied to the analysis of blood serum. True limit of detection in real samples is 0.01-0.02 mg/L and the limit of quantification (RSD 10%) is 0.03-0.07 mg/L using Se I 196.090 nm line at an integration time of 10-2 s. The method was tested by analysis of porcine blood serum and the serum reference material Seronorm MI 0181. (orig.)

  15. LEVELS OF SELECTED INDICATORS IN THE BLOOD SERUM OF HORSES AND THEIR CORRELATION DEPENDENCY

    OpenAIRE

    Peter Čupka; Marek Halo; Peter Massányi; Anton Kováčik; Robert Stawarz; Formicki Grzegorz

    2013-01-01

    In this study 26 sport horses (14 mares and 12 stallions) were included in the trial. The content of urea, total proteins (TP), glucose (GLU), cholesterol (CHOL), triglycerides (TG), bilirubin (BILI), AST, ALT, GGT and ALP was determined in the blood serum. Levels of monitoring indicators in stallions and mares were balanced. Significant difference (P

  16. Serum selenium concentration in maternal and umbilical cord blood. Relation to course and outcome of pregnancy

    DEFF Research Database (Denmark)

    Bro, S; Berendtsen, H; Nørgaard, J; Høst, A; Jørgensen, P J

    1988-01-01

    The present knowledge of the role of selenium in human fetal and neonatal development is sparse. In this study we measured serum selenium concentrations in maternal and umbilical cord blood from 500 Danish mothers at delivery, looking for a relationship between various maternal and fetal complica...

  17. Quality standards, safety and efficacy of blood-derived serum eye drops: A review.

    Science.gov (United States)

    van der Meer, Pieter F; Seghatchian, Jerard; Marks, Denese C

    2016-02-01

    Serum eye drops (SEDs) are being used increasingly to treat dry eye syndrome and persistent corneal epithelial defects, and are usually prescribed when conventional treatments fail. SEDs are commonly sourced from the patient's own blood via an autologous collection. Although SEDs are clearly beneficial, they are not available for those patients that cannot donate sufficient blood, and some centres are moving to allogeneic SEDs. Many studies have reported that both allogeneic and autologous SEDs are effective. However, few large randomised controlled trials have been conducted to date, and clinical evidence is therefore limited to smaller studies. Alternatives to serum are also being explored, such as platelet lysate and products made from platelet rich plasma, as they are a rich source of growth factors. This article reviews how some centres are approaching allogeneic collections for SEDs, and alternatives to serum that are currently being explored. PMID:26847866

  18. Blood Transfusion, Serum Ferritin, and Iron in Hemodialysis Patients in Africa

    Directory of Open Access Journals (Sweden)

    Leonard Kouegnigan Rerambiah

    2015-01-01

    Full Text Available Background and Objectives. There is no data analyzing the outcome of blood transfusions and oral iron therapy in patients with kidneys failure in sub-Saharan Africa. The present study aimed to fill that gap and assess the value of ferritin in the diagnosis of iron overload and deficiency. Design. From January to February 2012, we prospectively studied 85 hemodialysis patients (78% of males and 22% of females aged 20 to 79 years attending the Gabonese National Hemodialysis Centre. Results. Correlation studies showed (a a strong positive linear relationship between the number of blood transfusions and high serum ferritin in hemodialysis patient (Spearman r:0.74; P value: 0.0001; (b a weak association between the number of blood transfusions and serum iron concentrations (Spearman r:0.32; P value: 0.04; (c a weak association between serum ferritin and serum iron (Spearman r:0.32; P value: 0.003. Also, the strength of agreement beyond chance between the levels of ferritin and iron in the serum was poor (κ=0.14. The prevalence of iron overload was 10.6%, whereas the prevalence of iron deficiency was 2.3%, comparing (1 patients with a maximum of one transfusion not on iron therapy; (2 patients with a maximum of one transfusion on iron therapy; (3 polytransfused patients not on iron therapy; and (4 polytransfused patients on oral iron therapy. The “Kruskal-Wallis test” showed that ferritin levels varied significantly between the groups (P value: 0.0001. Conclusion. Serum ferritin is not reliable as a marker of iron overload. For patients undergoing regular transfusion we recommend routine serum ferritin measurement and yearly measurement of LIC.

  19. Multicomponent determination of 4-hydroxycoumarin anticoagulant rodenticides in blood serum by liquid chromatography with fluorescence detection.

    Science.gov (United States)

    Felice, L J; Chalermchaikit, T; Murphy, M J

    1991-01-01

    A sensitive liquid chromatographic method was developed for the analysis of 4-hydroxycoumarin anticoagulant rodenticides in blood serum. The method can simultaneously measure the serum levels of five anticoagulant rodenticides: brodifacoum, bromadiolone, coumatetralyl, difenacoum, and warfarin. Serum proteins are precipitated with acetonitrile and the supernatant is mixed with ethyl ether. The organic phase is separated, evaporated to dryness, and the residue subjected to chromatographic analysis. The anticoagulants are separated by reversed-phase gradient chromatography with fluorescence detection at an excitation wavelength of 318 nm and emission wavelength of 390 nm. Extraction efficiencies of 68.1 to 98.2% were obtained. The within-run precision (CV) ranged from 2.19 to 3.79% and the between-run precision (CV) from 3.72 to 9.57%. The anticoagulants can be quantitated at serum levels of 10 to 20 ng/mL. PMID:1943055

  20. Concentration of beta-carotene and vitamin A in blood serum of cows in peripartal period

    Directory of Open Access Journals (Sweden)

    Veličković Miljan

    2005-01-01

    Full Text Available In a herd of high-productive dairy cows of the Holstein breed, 40 cows were selected for an experiment. The animals were chosen at the period of advanced gravidity. Data on mean values of beta-carotene concentrations in blood serum of the cows show that significantly higher values (p<0.01 were established in advanced gravidity (x=9.53± 3.49 μmol/l in comparison with the values on calving day (x=5.69±3.14 μmol/l and in early puerperium (x=2.25±1.00 μmol/l. The average concentration of vitamin A in blood serum of cows varied approximately the same as the concentration of beta-carotene. The highest average concentration of vitamin A in blood serum of cows was determined in advanced gravidity (x=1.856±0.52 μmol/l, and the lowest in early puerperium (x=0.988±0.31 μmol/l (p<0.05. It can be concluded on the grounds of the results obtained in this work that the average concentration of beta-carotene and vitamin A in blood serum of cows in advanced gravidity and on the day of calving is within the limits of physiological values and in keeping with the values cited in literature, and that concentrations of beta-carotene and vitamin A in blood serum of cows two weeks after calving are significantly lower than the values obtained in advanced gravidity and on the day of calving and are lower than the physiological values most often reported in literature for this animal specie.

  1. The correlation between serum leptin and blood pressure after exposure to noise at work

    Directory of Open Access Journals (Sweden)

    Muayad S Rahma

    2013-01-01

    Full Text Available Several epidemiologic studies have reported that exposure to noise is associated with cardiovascular disease. The increased body weight is often associated with metabolic as well as increased blood pressure. The aim of this study is to investigate the correlation between the elevation of blood pressure and serum leptin hormones due to the effects of noise in the work place. A total of 80 volunteer males where included in this study with an age range between of 20 and 45 years, they were divided in two groups equally, the 1 st group were exposed to noise in the workplace while the 2 nd group were not. The individual noise exposure was determined by using a sound level meter. The range of noise was 80-100 dBA. Body Mass Index was also taken for each individual by a standard measure, blood pressure was measured by OMRON sphygmomanometer and serum leptin was measured through venous blood sample analysis enzyme linked immunosorbent assay. Spearman rank order correlation was used to examine the correlations between Blood pressure value (Systolic, Diastolic and Leptin. All the relationships between parameters showed a positive correlation. Systolic and diastolic blood pressure values had a significant correlation to leptin hormone level in comparison to the control. There was a significant relation between leptin and blood pressure. leptin effects on the sympathetic nervous system may provide a partial explanation. Therefore, Leptin might have diverse cardiovascular actions.

  2. Identification of transferrin as the principal neptunium-binding protein in the blood serum of rats

    Energy Technology Data Exchange (ETDEWEB)

    Wirth, R.; Taylor, D.M.; Duffield, J.

    1985-01-01

    The binding of 239Np(V) to blood serum components of rats was examined in vivo and in vitro. After gel filtration of the serum using a Sephacryl S-300 column, 98% of the applied activity appeared with protein fractions representing coeluted albumins and transferrin. A separation of the albumin- and transferrin-proteins by ion-exchange chromatography using DEAE-cellulose showed the 239Np being entirely bound to the iron-carrier protein transferrin. The high elution yields from the ion-exchange columns, greater than 90%, suggest that the binding may be quite strong. The binding capacity of transferrin for neptunium in vivo was found to decline when the iron level in blood serum was increased. Precipitation experiments showed that 84 +/- 2% of the 239Np was precipitated with 10% (w/v) trichloracetic acid, 77 +/- 3% with 90% ethanol but only 6 +/- 1% with saturated ammonium sulphate at pH 7.4. The available data indicate that as for plutonium, thorium, americium and curium, the iron transport protein, transferrin, may be the main carrier protein for neptunium in mammalian blood serum.

  3. National blood requirement, serum ALT and hepatitis in Ethiopian blood donors.

    Science.gov (United States)

    Zawde, D; Sisay, Y

    1991-10-01

    To appraise the national blood requirement and supply, and to determine the impact of alanine aminotransferase (ALT) and hepatitis B surface antigen (HBsAg) screening on the blood supply, 407 random blood donor sera were tested for HBsAg, human immunodeficiency virus (HIV), and ALT activity. HBsAg and anti-HIV antibody were determined by the enzyme linked immunosorbent assay (ELISA) technique using Hepanostica and Welcozyme kits, respectively. The Western Blot test was performed to confirm anti-HIV positive sera by the ELISA technique. ALT was determined by an automated photometer using ALAT kits and serologic testing for syphilis was done by the rapid plasma reagin (RPR) test. The amount of blood required in Ethiopia and the actual supply was calculated on the basis of the number and type of hospital beds in Addis Abeba and the amount of blood transfusions in units per hospital bed. The results showed that the combined donor and unit rejection rate was 34.6%. The annual blood requirement was 7 units for emergency and 4 units for nonemergency beds. The national blood requirement in 1989 was 64,350-80,000 units, but the supply met only a third of the requirement. The mean and 2SD cut off ALT levels were 28 and 69 IU/L, respectively. ALT was elevated in 9.1% of HBsAg positive but apparently healthy donors, while HBsAg screening eliminated 25% of those with elevated ALT activity. This data suggests that there is a serious blood shortage in Ethiopia and that the currently supplied blood is relatively unsafe in terms of hepatitis.(ABSTRACT TRUNCATED AT 250 WORDS) PMID:1659534

  4. Direct determination of sodium and potassium in blood serum by flow injection and atomic absorption spectrophotometry

    International Nuclear Information System (INIS)

    A simple and reliable method for the measurement of sodium and potassium in blood serum without any sample dilution by using flow injection and atomic absorption spectrophotometry is described. A sample throughout of 100 measurements per hour is possible. The coefficient of variation for within-run determination was about 1,14 and 2,36% for sodium and potassium, respectively, in serum samples (n=10). The method is easily adaptable to pediatric research, because of the low required sample volume of 5ul. (Author)

  5. Trace element analysis of human blood serum by neutron activation analysis

    International Nuclear Information System (INIS)

    An attempt was made to determine if there is any correlation between trace element concentrations in human blood serum and some specific diseases. The serum samples of the patients suffering from cancer, Down syndrome, and Banti syndrome were analyzed by the neutron activation method and compared with the trace element concentrations observed among clinically healthy men. The cancer patients had concentrations in Rb, Mn, Fe, Co, Cu, Zn, Al and Se below normal. The Down syndrome patients were found to have similar deficiencies in Cr, Mn, Fe, Co, Zn, Cu and Sb. (author)

  6. Serum cortisol level and its correlation to serum insulin and fasting blood sugar levels in patients with type 2 diabetes mellitus

    International Nuclear Information System (INIS)

    Objective: To investigate the change of serum cortisol levels and its correlation to blood sugar and serum insulin levels in patients with type 2 diabetes mellitus. Methods: Blood sugar with oxidase method and serum cortisol insulin levels with RIA (8 AM fasting specimen) were measured in 26 patients with type 2 diabetes mellitus and 30 controls. Results: The serum cortisol levels in the diabetic patients were significantly higher than those in the controls (P<0.01). The cortisol levels were positively correlated to the blood sugar levels (r=0.32, p<0.01), but not correlated to insulin levels. Conclusion: There were cortisol secretion disturbances in patients with type 2 diabetes

  7. Increased serum erythropoietin concentration after allogeneic compared with autologous blood transfusion.

    Science.gov (United States)

    Avall, A; Hyllner, M; Swolin, B; Bengtson, J p; Carlsson, L; Bengtsson, A

    2002-12-01

    Serum erythropoietin (sEPO) level is known to increase as hemoglobin (Hb) concentration decreases during and after preoperative autologous blood donation (PAD). The endogenous erythropoietin (EPO) production after allogeneic blood transfusion has not to our knowledge, been studied. The aim of the present study was to determine whether there is, after surgery, any change in sEPO concentration after allogeneic blood transfusion, and whether there is any difference in EPO response after autologous or allogeneic blood transfusion. Thirty-one patients approaching total hip-joint replacement surgery, were randomized to receive either allogeneic red blood cells (n = 15) or predeposited autologous whole blood transfusion (n = 16). The relationship between Hb, sEPO, and reticulocytes in the recipients were repeatedly analyzed before, during and after surgery. The Hb followed an expected pattern, with a decreased concentration after PAD in the autologous group, then in both groups after surgery. The sEPO concentration was significantly higher in the allogeneic than in the autologous group on day one and day 4-5 postoperatively. The reticulocyte level, on the contrary, was higher in the autologous patients before, one hour after, and one day after surgery. The study showed a greater increase in sEPO concentration after allogeneic blood transfusion than after autologous blood transfusion. There may be an inverse relationship between sEPO and the reticulocyte level. PMID:12509214

  8. Blood serum and BSA, but neither red blood cells nor hemoglobin can support vitellogenesis and egg production in the dengue vector Aedes aegypti

    Directory of Open Access Journals (Sweden)

    Kristina K. Gonzales

    2015-05-01

    Full Text Available Aedes aegypti is the major vector of dengue, yellow fever and chikungunya viruses that put millions of people in endemic countries at risk. Mass rearing of this mosquito is crucial for strategies that use modified insects to reduce vector populations and transmission of pathogens, such as sterile insect technique or population replacement. A major problem for vector mosquito mass rearing is the requirement of vertebrate blood for egg production since it poses significant costs as well as potential health hazards. Also, regulations for human and animal use as blood source can pose a significant obstacle. A completely artificial diet that supports egg production in vector mosquitoes can solve this problem. In this study, we compared different blood fractions, serum and red blood cells, as dietary protein sources for mosquito egg production. We also tested artificial diets made from commercially available blood proteins (bovine serum albumin (BSA and hemoglobin. We found that Ae. aegypti performed vitellogenesis and produced eggs when given whole bovine blood, serum, or an artificial diet containing BSA. Conversely, egg production was impaired after feeding of the red blood cell fraction or an artificial diet containing only hemoglobin. We also found that egg viability of serum-fed mosquitoes were comparable to that of whole blood and an iron supplemented BSA meal produced more viable eggs than a meal containing BSA alone. Our results indicate that serum proteins, not hemoglobin, may replace vertebrate blood in artificial diets for mass mosquito rearing.

  9. Blood serum and BSA, but neither red blood cells nor hemoglobin can support vitellogenesis and egg production in the dengue vector Aedes aegypti.

    Science.gov (United States)

    Gonzales, Kristina K; Tsujimoto, Hitoshi; Hansen, Immo A

    2015-01-01

    Aedes aegypti is the major vector of dengue, yellow fever and chikungunya viruses that put millions of people in endemic countries at risk. Mass rearing of this mosquito is crucial for strategies that use modified insects to reduce vector populations and transmission of pathogens, such as sterile insect technique or population replacement. A major problem for vector mosquito mass rearing is the requirement of vertebrate blood for egg production since it poses significant costs as well as potential health hazards. Also, regulations for human and animal use as blood source can pose a significant obstacle. A completely artificial diet that supports egg production in vector mosquitoes can solve this problem. In this study, we compared different blood fractions, serum and red blood cells, as dietary protein sources for mosquito egg production. We also tested artificial diets made from commercially available blood proteins (bovine serum albumin (BSA) and hemoglobin). We found that Ae. aegypti performed vitellogenesis and produced eggs when given whole bovine blood, serum, or an artificial diet containing BSA. Conversely, egg production was impaired after feeding of the red blood cell fraction or an artificial diet containing only hemoglobin. We also found that egg viability of serum-fed mosquitoes were comparable to that of whole blood and an iron supplemented BSA meal produced more viable eggs than a meal containing BSA alone. Our results indicate that serum proteins, not hemoglobin, may replace vertebrate blood in artificial diets for mass mosquito rearing. PMID:26020000

  10. Detection and identification of antinuclear autoantibodies in the serum of normal blood donors.

    Science.gov (United States)

    de Vlam, K; De Keyser, F; Verbruggen, G; Vandenbossche, M; Vanneuville, B; D'Haese, D; Veys, E M

    1993-01-01

    The occurrence of antinuclear antibodies (ANA) in the serum of 485 healthy volunteer blood donors was assessed. Sixty two sera displayed nuclear immunofluorescence staining on Hep-2 cells using a polyvalent anti-Ig conjugate. In general, the titer of these antibodies was low (42/62 sera displaying a titer lower than or equal to 1:80). In only 23 sera were the ANA of the IgG isotype, which is the more disease-related immunoglobulin class of autoantibodies. In order to define the frequency of antibodies to extractable nuclear antigens and dsDNA within this population, sera were further analyzed by counterimmunoelectrophoresis. Western blot and the Crithidia luciliae assay. One serum displayed weak antids DNA reactivity; another serum had anti-SSA/Ro activity. On Western blot several patterns were found. They could not be identified with any of the available reference antisera. PMID:8403584

  11. Study of serum malondialdehyde and whole blood reduced glutathione in emphysema patients

    Directory of Open Access Journals (Sweden)

    Nagaraj R.Shetkar, Anand Pyati, Rajesh CS

    2013-04-01

    Full Text Available Background: Emphysema is associated with high incidence of morbidity and mortality. The imbalance between oxidants and antioxidants is thought to play an important role in the pathogenesis of Emphysema. Methods: A total number of 80 subjects comprising of 40 healthy controls and 40 Emphysema cases were studied. In all the subjects, serum levels of malondialdehyde (MDA as a biomarker of lipid peroxidation and antioxidant whole blood reduced glutathione (GSH were estimated. Results: The level of whole blood reduced glutathione was significantly decreased in emphysema patients when compared to controls. Serum MDA was significantly increased in Emphysema patients when compared to controls. Conclusion: The presence of increased systemic oxidative stress in emphysema patients seems to be associated with current active smoking and systemic inflammation. The decrease in antioxidants levels appears to be mainly a consequence of increased oxidative stress. This suggests that oxidative stress is likely to be involved in pathogenesis of emphysema.

  12. CYTOKINES IN LACRIMAL FLUID AND BLOOD SERUM: EARLY BIOMARKERS OF AGE-RELATED MACULAR DEGENERATION

    Directory of Open Access Journals (Sweden)

    O. S. Slepova

    2015-06-01

    Full Text Available The article presents results of multiplex cytokine assays in blood serum and lacrimal fluid at the initial and intermediate stages of age-related macular degeneration (AMD. Some features of local and systemic disturbances in the cytokine profile were detected in these patients. It was revealed that the initial stage of AMD was associated with elevated IL-17 levels in lacrimal fluid, along with imbalance between the local increase and systemic decrease of TGF-β1 amounts. Intermediate-stage AMD was associated with increased levels of the most cytokines assayed (except of TGF-β1 in blood serum and lacrimal fluid, thus suggesting stimulation of both pro-inflammatory and angiogenic responses, like as activation of anti-inflammatory and anti-infective factors. 

  13. Electrophoretic pattern of blood serum proteins of some of the vertebrates of Pakistan

    International Nuclear Information System (INIS)

    The electrophoretic pattern of blood serum proteins of some of the common fishes e.g. Catla catla, Cirrhina mrigala, Channa punctatus, Channa marulius, Wallago attu, Heterop-neustes fossilis; amphibia e.g., Rana tigrina, Rana cyanophlyctis, Bufo melanostictus; reptiles e.g. Varanus bengalensis, Uromastix hardwickii; birds e.g. Columba livia, Gallus domesticus, Passer domestica, Anas platyrhynchos; and mammals e.g. Homo sapiens, Mus musculus, Lepus cuniculus have been described. The mobility of proteins of blood sera has been studied over cellulose acetate paper and then a comparative pattern analysed

  14. Development of radiochemical method of analysis of binding of tritium labeled drotaverine hydrochloride with human blood serum albumin

    International Nuclear Information System (INIS)

    Full text: The albumin, being a basic functional linkage of numerous endogenous and exogenous substances is the most important protein of blood plasma. At the diseases connected to liver disfunction, collected in blood metabolite reduce connecting ability of albumino. The aim of the present research was a development of radiochemical method of determination of ability of albumin to bind the tritium labeled preparation drotaverine hydrochloride (no - spa). We had developed a micromethod of definition of connecting ability of albumin, allowing to analyse 20 mkl of blood serum. The method consists in incubation of tritium labeled drotaverine hydrochloride with blood serum in vitro, the following fractionation of serum proteins by gel - filtration on a microcolumn with Sephadex G-25, and direct measurement of the radioactivity connected to fraction of proteins of blood serum. The method has been tested on a series of blood serum of control group of healthy people and on a series of blood serum of patients with hepatitis B. We received quantitative characteristics of binding of drotaverine hydrochloride with albumin of patients with hepatitis B. It was preliminary established that binding ability of serum albumin of children with various forms of acute virus hepatitis tends to decrease in comparison with group of the control. Advantage of the developed radiochemical method is high precision and the high sensitivity of detection of infringement of binding ability of albumin. Application of tritium labeled drotaverine hydrochloride allows to measure directly levels of binding of a preparation with albumin

  15. Effect of mastitis on macro-minerals of bovine milk and blood serum in Sudan

    OpenAIRE

    El Zubeir, I.E.M.; O.A.O. ElOwni; G.E. Mohamed

    2005-01-01

    Milk and blood serum from clinically mastitis infected, subclinically mastitis infected and healthy Friesian cows (15 samples from each of 3 groups) were evaluated for macrominerals (sodium, potassium, calcium, magnesium and phosphorus). The milk from cows infected with subclinical mastitis revealed a significant decrease in potassium (P < 0.001) and a significant increase in sodium and phosphorus content (P < 0.01). Similarly, the milk from cows with the clinical form of the disease sh...

  16. Blood pressure and serum cholesterol among male Ethiopian immigrants compared to other Israelis.

    OpenAIRE

    Green, M S; Etzion, T.; Jucha, E

    1991-01-01

    STUDY OBJECTIVE--The aim was to determine the effect of migration from a non-industrialised to an industrialised society on age related changes in blood pressure and serum cholesterol. DESIGN--The study was a comparison of major risk factors for cardiovascular disease in cross sectional surveys in two groups: Ethiopian immigrants and Israeli industrial employees. SETTING--Ethiopian immigrants were examined at Army induction centres in Israel; industrial employees were examined in the course o...

  17. Inhibition of bacterial aggregation by serum- and blood-derived proteins.

    OpenAIRE

    Malamud, D; Brown, C; Goldman, R

    1984-01-01

    Human and animal sera contain potent inhibitors of saliva-mediated aggregation of oral streptococci. The inhibitors consist of a high-molecular-weight heat-labile factor and a lower-molecular-weight heat-activated factor. The latter appears to be serum albumin. Analyses of purified blood-derived proteins indicated that several high-molecular-weight proteins (fibrinogen, fibronectin, and ferritin) were able to inhibit aggregation at low concentrations. These data suggest that high-molecular-we...

  18. Platelet Component of Hemostasis System and the Content of Homocysteine in Blood Serum in Coxarthrosis

    OpenAIRE

    Fedoseeva I.А.; Ezhov I.Y.; Tcharykova I.N.

    2011-01-01

    The aim of the investigation is to study the content of homocysteine in blood serum and the analysis of thrombocyte aggregation activity in patients suffering from degenerative-dystrophic diseases of coxofemoral joint (coxarthrosis). Materials and Methods. There have been examined 155 patients with coxarthrosis and 45 healthy men and women aged 45—65 years old. The platelet aggregation ability has been studied using adenosine diphosphate (ADP) in small doses (1.25 mkg/ml) and collagen (2....

  19. Correlations between meat quality and components of blood serum in steers fattened

    OpenAIRE

    Djagra, Ida Bagus; Wada, Hiroshi

    1980-01-01

    In fattened steers of Holstein breed correlation coefficients between factors of the carcass, and between roast grade and size of selected body parts were studied. Study of correlation between roast grade and levels of serumal components of blood taken at slaughter was carried out in the steer above mentioned. Roast grade had minus significant correlation to fat cover depth and daily gain, and plus significant correlation to hips width and thurls width. Correlations between roast grade and co...

  20. Characterization of monoclonal gammopathy of undetermined significance by calorimetric analysis of blood serum proteome.

    Directory of Open Access Journals (Sweden)

    Francisca Barceló

    Full Text Available Monoclonal gammopathy of undetermined significance (MGUS is a premalignant proliferative disorder that may progress to multiple myeloma, a malignant plasma cell neoplasia. We evaluated differential scanning calorimetry (DSC as an experimental tool for differentiating serum samples of MGUS patients from healthy individuals. DSC thermograms can be used for monitoring changes in the serum proteome associated with MGUS. MGUS patients showed great variability in serum thermogram characteristics, which depended on the IgG, IgA or IgM isotypes and/or the κ or λ light chains. Thermogram feature parameters distinguished patients with MGUS from healthy people. Serum samples, named as non-MGUS, were also collected from patients with subjacent immunological pathologies who were discarded of having MGUS through serum immunofixation. They were used to verify the sensitivity of DSC for discriminating MGUS from related blood dyscrasias. Only some DSC thermogram feature parameters differentiated, to a lesser extent, between MGUS and non-MGUS individuals. We contemplate DSC as a tool for early diagnosis and monitoring of MGUS.

  1. Serum levels of perfluoroalkyl compounds in human maternal and umbilical cord blood samples

    International Nuclear Information System (INIS)

    Perfluoroalkyl compounds (PFCs) are end-stage metabolic products from industrial flourochemicals used in the manufacture of plastics, textiles, and electronics that are widely distributed in the environment. The objective of the present study was to quantify exposure to perfluorooctane sulfonate (PFOS), perfluorooctanoate (PFOA), perfluorodecanoic acid (PFDeA), perfluorohexane sulfonate (PFHxS), perfluoroheptanoic acid (PFHpA), and perfluorononanoic acid (PFNA) in serum samples collected from pregnant women and the umbilical cord at delivery. Pregnant women (n=101) presenting for second trimester ultrasound were recruited and PFC residue levels were quantified in maternal serum at 24-28 weeks of pregnancy, at delivery, and in umbilical cord blood (UCB; n=105) by liquid chromatography-mass spectrometry. Paired t-test and multiple regression analysis were performed to determine the relationship between the concentrations of each analyte at different sample collection time points. PFOA and PFOS were detectable in all serum samples analyzed including the UCB. PFOS serum levels (mean±S.D.) were significantly higher (p<0.001) in second trimester maternal serum (18.1±10.9 ng/mL) than maternal serum levels at delivery (16.2±10.4 ng/mL), which were higher than the levels found in UCB (7.3±5.8 ng/mL; p<0.001). PFHxS was quantifiable in 46/101 (45.5%) maternal and 21/105 (20%) UCB samples with a mean concentration of 4.05±12.3 and 5.05±12.9 ng/mL, respectively. There was no association between serum PFCs at any time point studied and birth weight. Taken together our data demonstrate that although there is widespread exposure to PFCs during development, these exposures do not affect birth weight

  2. Serum zinc and copper concentrations in maternal and umbilical cord blood. Relation to course and outcome of pregnancy

    DEFF Research Database (Denmark)

    Bro, S; Berendtsen, H; Nørgaard, J; Høst, A; Jørgensen, P J

    1988-01-01

    serum zinc and copper concentrations in maternal and umbilical cord blood from 500 Danish mothers at delivery, looking for an association between serum zinc and copper levels and various maternal and foetal complications. Preterm infants (n = 30) had significantly lower serum copper concentrations than...... reference infants (n = 346) (p = 0.01), whereas there was no difference in serum zinc concentrations. Mothers of preterm infants (n = 34) did not differ in serum zinc or copper concentrations from reference mothers (n = 220). Small for date infants (n = 37) and mothers of small for date infants (n = 47) had...

  3. Synthesis of fluorine-18 radio-labeled serum albumins for PET blood pool imaging

    International Nuclear Information System (INIS)

    We sought to develop a practical, reproducible and clinically translatable method of radiolabeling serum albumins with fluorine-18 for use as a PET blood pool imaging agent in animals and man. Fluorine-18 radiolabeled fluoronicotinic acid-2,3,5,6-tetrafluorophenyl ester, [18F]F-Py-TFP was prepared first by the reaction of its quaternary ammonium triflate precursor with [18F]tetrabutylammonium fluoride ([18F]TBAF) according to a previously published method for peptides, with minor modifications. The incubation of [18F]F-Py-TFP with rat serum albumin (RSA) in phosphate buffer (pH 9) for 15 min at 37–40 °C produced fluorine-18-radiolabeled RSA and the product was purified using a mini-PD MiniTrap G-25 column. The overall radiochemical yield of the reaction was 18–35% (n = 30, uncorrected) in a 90-min synthesis. This procedure, repeated with human serum albumin (HSA), yielded similar results. Fluorine-18-radiolabeled RSA demonstrated prolonged blood retention (biological half-life of 4.8 hours) in healthy awake rats. The distribution of major organ radioactivity remained relatively unchanged during the 4 hour observation periods either by direct tissue counting or by dynamic PET whole-body imaging except for a gradual accumulation of labeled metabolic products in the bladder. This manual method for synthesizing radiolabeled serum albumins uses fluorine-18, a widely available PET radionuclide, and natural protein available in both pure and recombinant forms which could be scaled up for widespread clinical applications. These preclinical biodistribution and PET imaging results indicate that [18F]RSA is an effective blood pool imaging agent in rats and might, as [18F]HSA, prove similarly useful as a clinical imaging agent

  4. Blood cell characteristic, hematological and serum biochemistry values of Painted Stork (Mycteria leucocephala

    Directory of Open Access Journals (Sweden)

    Worapol Aengwanich

    2002-07-01

    Full Text Available Blood cell characteristics, hematological and serum biochemistry values were examined in 10 mature healthy Painted Storks (Mycteria leucocephala (5 males and 5 females from Khow Khoew Open Zoo, Chonburi province, Thailand. The morphology of erythrocytes, leukocytes and thrombocytes were similar to those of other avian species, but oval shaped granules of heterophils were less stained by Giemsa-Wright solution. Erythrocytes, hemoglobin, packed cell volume, mean corpuscular volume, mean corpuscular hemoglobin, mean corpuscular hemoglobin concentration, total white blood cells, percentage of leukocytes (lymphocyte, heterophil, monocyte, eosinophil, basophil, thrombocytes, serum glucose, serum creatinine, serum glutamic oxaloacetic transaminase and serum glutamic pyruvic transaminase values of Painted Storks were 3.24 + 0.82 106cells / μl, 0.82 106cells /μl, 17.67 + 1.56 g/dl, 42.40 + 3.57 %, 139.47 + 38.70 fl, 58.56 + 18.24 g/dl, 41.76 + 2.90 pg, 3.88 + 2.60 104cells /μl, 76.10 + 10.28 %, 10.60 + 9.49 %, 0.00 + 0.00 %, 12.00 + 8.54 %, 1.00 + 1.33 %, and 1.29 + 0.42104cells /μl, 270.50 + 42.85 mg/dl, 6.19 + 1.70 mg/dl, 249.47 + 42.19 IU/L and 124.29 + 8.81 IU/L, respectively. Total erythrocytes and mean corpuscular volume (MCV differed significantly between male and female Painted Stork (P < 0.05.

  5. Serum lipids and apolipoprotein B values, blood pressure and pulse rate in anorexia nervosa.

    Science.gov (United States)

    Sánchez-Muniz, F J; Marcos, A; Varela, P

    1991-01-01

    Some risk factors associated with coronary heart disease (CHD) were evaluated in patients with different types of anorexia nervosa (AN). Anthropometric parameters, serum cholesterol, triglycerides and apoprotein (apo) B values, blood pressure and pulse rate were tested in 29 young female patients and 16 controls. Cholesterol, triglycerides and apo B were higher at the acute period of the illness (AN1), whereas at the chronic period the values of these parameters tended to normalize. Triglycerides were higher in patients who binge ate (bulimarexia). Systolic blood pressure decreased in all types of AN, while diastolic blood pressure decreased only in AN1; pulse rate was not altered. According to cholesterol and apo B values, AN patients may be at risk of CHD if they remain at low body weight. PMID:1855497

  6. HMSRP Hawaiian Monk Seal Blood Values (Establishing hematology and serum chemistry reference ranges for wild Hawaiian Monk Seals)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Hematology and serum chemistry evaluations are essential to a patients comprehensive health exam by providing measures of organ function, blood cell volume and...

  7. Comparison of serum, salivary, and rapid whole blood diagnostic tests for Helicobacter pylori and their validation against endoscopy based tests.

    OpenAIRE

    Reilly, T G; Poxon, V; Sanders, D S; Elliott, T S; Walt, R P

    1997-01-01

    BACKGROUND: A rapid, reliable, and accurate test for the diagnosis of infection with Helicobacter pylori is needed for screening dyspeptic patients before referral for endoscopy. AIM: To compare a new rapid whole blood test (Helisal rapid blood, Cortecs), two serum enzyme linked immunosorbent assays (ELISAs; Helico-G, Shield and Helisal serum, Cortecs), and a salivary assay (Helisal saliva, Cortecs), with slide biopsy urease, 13C-urea breath test, and histology. METHODS: Three hundred and thr...

  8. Influence of normal blood serum on the uptake and organificition of iodine-131 by thyroid cell culture

    International Nuclear Information System (INIS)

    The effect of normal blood serum of cattle on the levels of uptake and organification of iodine-131 by the primary culture of thyroid cells of newborn porcine has been studied. The negative dose-dependent effect suggests the influence of biologically active substances of blood serum on the regulation of these processes both at the receptor and post receptor levels, but the mechanism of a such effect is yet unknown

  9. Body Fatness and Risk for Elevated Blood Pressure, Total Cholesterol, and Serum Lipoprotein Ratios in Children and Adolescents.

    Science.gov (United States)

    Williams, Daniel P.; And Others

    1992-01-01

    Examines the relationship between body fat percent and risk for elevated blood pressure, serum total cholesterol, and serum lipoprotein ratios in 1,230 African-American and 2,090 white 5-18 year olds (1,667 males and 1,653 females). Results support body fatness standards in children and adolescents as cardiovascular risk factors. (SLD)

  10. GUAR GUM EFFECTS ON BLOOD SERUM LIPIDS AND GLUCOSE CONCENTRATIONS OF WISTAR DIABETIC RATS1

    Directory of Open Access Journals (Sweden)

    DARIO FRIAS Andrea

    1998-01-01

    Full Text Available The effects of guar gum derived from the endosperm of Cyamopsis tetragonoloba (75% soluble fiber, 7.6% insoluble fiber, 2.16% crude protein, 0.78% total lipids, 0.54% ash and 9.55% moisture on diabetic rats were studied concerning food intake, body weight gain, blood serum cholesterol, triacylglycerols, glucose, LDL-, and HDL-cholesterol concentrations. The effect of gum on indexes of protein absorption and utilization was also investigated. Diets containing 0%, 10% and 20% (w/w guar gum were fed to diabetic rats for 28 days. In spite of the fact that diabetes elevated blood lipids in all animals, guar gum diet significantly decreased (p <0.05 serum concentrations of cholesterol and triacylglycerols. Furthermore, a concomitant increase in HDL-cholesterol with a substancial elevation of the HDL/LDL cholesterol ratio was found. The most significant result in this assay was the drastic reduction of blood glucose in diabetic rats treated with guar gum diet. The gum promoted a general improvement in the condition of the diabetic rats, in body weight gain and indexes of protein absorption and utilization. The results of this research suggest that guar gum, at concentrations equal to or higher than 10%, should be effective in the treatment of hypercholesterolemia and diabetes, in humans.

  11. Investigation the effect of Commiphora mukul on blood glucose and Serum lipid profile in diabetic rats

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    leila Shirazi

    2015-01-01

    Full Text Available Background & aim: The decrease of serum glucose level and lipids in diabetic patients is clinically significant. The purpose of this study was to determine the effect of Commiphora mukul extract on blood sugar and lipid profile in diabetic rats. Methods: In the present experimental study, thirty-two male Wistar rats were randomly divided into four groups: control, control treated with the extract, diabetic and diabetic treated with the extract. Diabetes was induced by intraperitoneal injection of Streptozotocin. In an eight week period, the control group of normal saline and the control group and diabetic recipient extract of CM oleo gum blue resin was given by gavage. Treatment resumed eight weeks with onset of hyperglycemia. The control and diabetic control groups received normal saline orally. Extract treated control and extract treated diabetic groups received extract of Commiphora mukul gum (300 mg/kg P.O. daily by gavage. At the end of this period, blood samples were collected from each rat and biochemical tests for investigation of glucose level and lipid profile was performed. One- way analysis of variance (ANOVA statistical test and Post-hoc test Tukey’s were used for data analysis Results: The study indicated that diabetes increases the serum levels of glucose, cholesterol, triglycerides, LDL and HDL. Administration of Commiphora mukul gum extract in diabetic groups significantly decreased the serum level of glucose, cholesterol(p<0.01 and triglyceride and LDL(p<0.001 and increased HDL(p<0.01. Conclusion: Commiphora mukul gum extract may well improve undesirable effects of diabetes on serum level of glucose, cholesterol, triglyceride, LDL and HDL.

  12. Analysis of blood serum of lung cancer patients using particle induced X-ray emission

    International Nuclear Information System (INIS)

    Trace elemental imbalance in human beings is postulated to exert action, directly or indirectly, on the carcinogenic process. The objective of this study was to evaluate the levels of trace elements in blood serum samples of lung cancer patients and analyze their alteration with respect to healthy controls. Particle induced X-ray emission (PIXE), a well established method for elemental analysis, was used to identify and quantify trace elements in the blood serum samples of the studied groups. The PIXE measurements were carried out using 2.5 MeV collimated proton beam from the 3 MV Tandem Pelletron Accelerator at Ion Beam Laboratory, Institute of Physics, Bhubaneswar, India. The serum of the cancerous group displayed increased concentrations of Ti, Ni, and Cu but lowered concentrations of V, Cr, Mn, Fe, Co, Zn, and Se. Statistically significant differences were found for serum Cr (p < 0.01), Fe (p < 0.0005), Ni (p < 0.05), Cu (p < 0.00005), and Zn (p < 0.0005) between the two studied groups. The copper to zinc ratio for the lung cancer group was 2.24 ± 0.39, which was almost three times the value for normal subjects (0.77 ± 0.14). The observed alterations are discussed with respect to the possible mechanisms by which these elements might influence the carcinogenic process. Significant reduction in mortality from lung cancer can be achieved by advances in early diagnosis and implementation of multidisciplinary treatment programmes leading to improvement of survivorship and better quality of life. It is expected that similar studies from all corners of the world would ultimately lead to the development of novel therapeutic agents to treat lung cancer. (author)

  13. DRIED BLOOD/SERUM SPOT TOTAL CHOLESTEROL ESTIMATION AS AN ALTERNATIVE TO FRESH SERUM TOTAL CHOLESTEROL: AN ANSWER OR A QUESTION IN ITSELF?

    OpenAIRE

    Pushpa,; Raghunath; Nimisha

    2015-01-01

    INTRODUCTION: Surveillance for risk factors of heart diseases, like increased blood sugar, cholesterol becomes difficult in places with inadequate lab facilities due to difficulty in sample collection, transportation and processing. Feasibility of using dried blood/seru m spots in such situations can be thought of as an alternative to fresh serum total cholesterol, as sample collection does not require much expertise. AIM: The aim of this study is to determine whether ...

  14. Patients blood serum ferritin concentrations changes associated with Caesium-137 incorporation

    International Nuclear Information System (INIS)

    The results of radioimmune study of ferritin and carcinoembryonic antigen in 60 persons: 35 those who took part in liquidation of the Chernobyl accident and incorporated 25.9-70.4 MBq of Cesium-137,25 residents of Rivno Region with 7.4-203.5 MBq of the radionuclide in the organism are reported. The increased concentration of ferritin and carcinoembryonic antigen in blood serum was noted to be determined more often in the residents of the areas polluted with Caesium-137 than in liquidators. The tumor markers levels depended to some extent on bad habits (smoking, alcohol) and existing chronic diseases of the alimentary system

  15. Clinical significance of determination of serum leptin, insulin levels and blood sugar in pregnant women with glucose metabolism disturbances

    International Nuclear Information System (INIS)

    Objective: To investigate the changes of serum leptin, insulin levels and blood sugar contents in pregnant women with gestational glucose metabolism disturbances. Methods: Fasting and 3h after oral 50g glucose serum levels of leptin were measured with RIA in 36 pregnant women with glucose metabolism disturbances (gestational diabetes mellitus or gestational impaired glucose tolerance) and 34 controls. Also, fasting serum insulin levels (with CLIA) and blood sugar contents 1h after oral 50 glucose (with glucose oxidase method) were determined in all these subjects. Results: 1. Serum levels of leptin in pregnant women with glucose metabolism disturbances were 14.9 ± 4.3 μg/L (vs controls 9.8 ± 1.7 μg/L, P<0.01). 2. The serum levels of insulin and 1 h post - 50g glucose blood sugar contents in pregnant women with glucose metabolism disturbances were 12.9±4.3mU/L and 11.0±1.4mmol/L respectively, which were both significantly positively correlated with the serum leptin levels (r=0.835, r=0.758 respectively) (vs levels in controls: 8.45±3.0mU/L and 7.84±1.3mmol/L). Conclusion: Elevation of fasting serum levels of leptin was demonstrated in pregnant women with glucose metabolism disturbances and the level of leptin was positively correlated with that of insulin and blood sugar. (authors)

  16. Electrophoretic pattern of blood serum proteins and enzymes activity of house sparrow (Passer domesticus in Baghdad (Iraq

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    Faris A. Al-Obaidi

    2011-04-01

    Full Text Available The objective of this study was to determine the electrophoretic pattern of blood serum proteins and the activity of some blood serum enzymes included GOT, GPT and AP of house Sparrow (Passer domesticus in Baghdad, Iraq. Seventeen (9 males and 8 females individuals of house sparrow were collected from different regions of Baghdad. Samples of 1.0 ml of whole blood were taken from the wing vein from individuals to determined electrophoretic pattern of serum proteins and enzymes in three replicates for each sex within species. Results revealed that house sparrow blood serum proteins were separated into seven different regions, these bands were pre- albumen (3.68%, albumen (13.20%, post–albumen (8.37%, α–globulin (4.54%, β–globulin (27.49%, γ–globulin (30.15% and transferrin (12.59%. Electrophoretic pattern of serum proteins were differed due to sex: females dominated males in pre–albumen, γ–globulin and total albumens. House sparrow blood serum GOT, GPT and AP enzymes activity were 38.4, 10.9 and 42.05 U/L respectively. Males had higher activity of GOT and GPT enzymes compared to females.

  17. Alterations of blood serum parameters in patients with chronic hematogenous osteomyelitis

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    Sadrudin Magomedov

    2015-10-01

    Full Text Available Objective: To examine metabolic disorders of major components of organic basis of bone tissue in patients with chronic hematogenous osteomyelitis and response to surgical treatment. Methods: The cubital vein puncture was conducted to take blood for analysis in patients with chronic hematogenous osteomyelitis. The activity of collagenase and hyaluronidase, elastin, elastase and total content of glycosaminoglycans were measured in blood serum. Results: The study revealed an enhancement of catabolic phase of metabolism of the main components in bone organic matrix during the relapse of inflammation. It was evidenced by indicators reflecting the synthetic and catabolic phases of the main components of the connective tissue collagen and glycosaminoglycans. The effective therapeutic treatments led to the reduction and normalization of studied compounds. Conclusions: The initial development of hematogenous osteomyelitis happens in a background of metabolic disorders of the main components of organic matrix of bone tissue, and normalizes upon effective therapy.

  18. Alterations of blood serum parameters in patients with chronic hematogenous osteomyelitis

    Institute of Scientific and Technical Information of China (English)

    Sadrudin Magomedov; Larisa Polishchuk

    2015-01-01

    Objective:To examine metabolic disorders of major components of organic basis of bone tissue in patients with chronic hematogenous osteomyelitis and response to surgical treatment. Methods: The cubital vein puncture was conducted to take blood for analysis in patients with chronic hematogenous osteomyelitis. The activity of collagenase and hyaluronidase, elastin, elastase and total content of glycosaminoglycans were measured in blood serum. Results: The study revealed an enhancement of catabolic phase of metabolism of the main components in bone organic matrix during the relapse of inflammation. It was evidenced by indicators reflecting the synthetic and catabolic phases of the main components of the connective tissue collagen and glycosaminoglycans. The effective therapeutic treatments led to the reduction and normalization of studied compounds. Conclusions: The initial development of hematogenous osteomyelitis happens in a background of metabolic disorders of the main components of organic matrix of bone tissue, and normalizes upon effective therapy.

  19. X-ray fluorescence analysis of trace elements in blood serum from diabetic patients

    International Nuclear Information System (INIS)

    Full text: The wave dispersive X-ray fluorescence (WDXRF) technique was used for analysis of trace elements in blood serum from 23 nondiabetic healthy individuals (control group) and 58 type II diabetic patients who attended the diabetic clinics. The elements Na, Mg, Al, P, S, Cl, K, Ca, Cr, Mn, Fe, Ni, Cu, Zn, Se, Mo, Cd, Hg and Pb were identified and their concentrations were estimated. Physical basis of used analytical methods, experimental set-up and the procedure of sample preparation are described. The maximum increase in trace elements in the blood samples of the workers compared with the control group was observed for Pb but also for Rb, As and Br

  20. Comparison of human whole blood, plasma, and serum matrices for the determination of perfluorooctanesulfonate (PFOS), perfluorooctanoate (PFOA), and other fluorochemicals

    International Nuclear Information System (INIS)

    Interest in human exposure to perfluorinated acids, including perfluorobutanesulfonate (PFBS), perfluorohexanesulfonate (PFHS), perfluorooctanesulfonate (PFOS), and perfluorooctanoate (PFOA) has led to their measurement in whole blood, plasma and serum. Comparison of measurements in these different blood-based matrices, however, has not been rigorously investigated to allow for across-matrix comparisons. This research evaluated concentrations of PFBS, PFHS, PFOS, and PFOA in whole blood collected in heparin (lithium) and ethylenediamine tetraacetic acid (EDTA), plasma samples collected in heparin and EDTA, and serum (from whole blood allowed to clot). Blood samples were collected from 18 voluntary participants employed at 3M Company. Solid phase extraction methods were used for all analytical sample preparations, and analyses were completed using high-pressure liquid chromatography/tandem mass spectrometry methods. Serum concentrations ranged from: limit of quantitation (LOQ, 5 ng/mL) to 25 ng/mL for PFBS; LOQ (5 ng/mL) to 75 ng/mL for PFHS; LOQ (5 ng/mL) to 880 ng/mL for PFOS; and LOQ (5 or 10 ng/mL) to 7320 ng/mL for PFOA. Values less than the LOQ were not included in the statistical analyses of the mean of the ratios of individual values for the matrices. PFBS was not quantifiable in most samples. Serum to plasma ratios for PFHS, PFOS, and PFOA were 1:1 and this ratio was independent of the level of concentrations measured. Serum or plasma to whole blood ratios, regardless of the anticoagulant used, approximated 2:1. The difference between plasma and serum and whole blood corresponded to volume displacement by red blood cells, suggesting that the fluorochemicals are not found intracellularly or attached to the red blood cells

  1. Relationship of serum 25-hydroxyvitamin D and parathyroid hormone levels with blood pressure in 1403 community adults

    Institute of Scientific and Technical Information of China (English)

    王睿

    2014-01-01

    Objective To investigate the association of serum25-hydroxyvitamin D(25OHD),parathyroid hormone(PTH),blood glucose,blood lipid,uric acid,obesity,insulin etc with blood pressure.Methods A total of 1403 residents aged(45.3±14.4)years were enrolled from Guiyang residents during a healthy survey in 2009.The standard questionnaire survey and physical examination were performed in all subjects.The fasting plasma glucose,postprandial 2 h plasma glucose(2h PG),triglyceride,high density lipoprotein-cholesterol,serum

  2. An Investigation of the Effects of Ivermectin on Blood Serum, Semen Hyaluronidase Activities and Spermatological Characteristics in Sheep

    OpenAIRE

    TANYILDIZI, Sadettin

    2002-01-01

    This study was carried out to investigate the effects of ivermectin on the hyaluronidase activities of blood serum and semen and on the sperm characteristics in sheep. In this investigation 18 sheep, at the age of 2 years and weighing between 50-60kg were used. After the administration of ivermectin subcutanously at a dose of 0.2 mg/kg, semen and blood samples were taken at different times. The hyaluronidase activities of blood serum and semen samples in sheep were determined to increase sign...

  3. The relationship between serum lipid levels, high blood pressure and obesity in children

    Directory of Open Access Journals (Sweden)

    Meltem Kurtuncu

    2014-03-01

    Full Text Available Objective: The purpose of this study was to determine serum lipid, cholesterol and obesity levels in healthy children, and then to explore the relationships between these factors. Methods: The sample group consisted of 103 students at Gelik Elementary School in Zonguldak, Turkey who was willing to participate in the study, had not been medically diagnosed with a chronic ailment, and were not taking regular medications. Results: When the students' mean BMI (Body Mass Index measurements were considered, it was found that mean BMI at 13 years of age (21.03 ± 0.23 was higher than at 14 years (20.05 ± 0.20 and that this difference was strongly significant (p=0.002. When the relationships between the students' height, weight, BMI, blood pressure, FBS (Fasting Blood Sugar, cholesterol, LDL (Low Density Lipoprotein, HDL (High Density Lipoprotein measurements were considered, it was seen that there was a positive significant relationship between height-weight (r=0.472; p<0.001, height-blood pressure (r=0.432; p<0.001, and height-FBS (r=0.332; p=0.001. Conclusions: The conclusion drawn was that monitoring blood pressure, cholesterol, LDL and HDL levels in childhood medical examinations is not only important in terms of identifying obesity, but also in identifying nutritional mistakes and deficiencies at an early stage.

  4. Methyl-binding domain protein-based DNA isolation from human blood serum combines DNA analyses and serum-autoantibody testing

    Directory of Open Access Journals (Sweden)

    Jungbauer Christof

    2011-09-01

    Full Text Available Abstract Background Circulating cell free DNA in serum as well as serum-autoantibodies and the serum proteome have great potential to contribute to early cancer diagnostics via non invasive blood tests. However, most DNA preparation protocols destroy the protein fraction and therefore do not allow subsequent protein analyses. In this study a novel approach based on methyl binding domain protein (MBD is described to overcome the technical difficulties of combining DNA and protein analysis out of one single serum sample. Methods Serum or plasma samples from 98 control individuals and 54 breast cancer patients were evaluated upon silica membrane- or MBD affinity-based DNA isolation via qPCR targeting potential DNA methylation markers as well as by protein-microarrays for tumor-autoantibody testing. Results In control individuals, an average DNA level of 22.8 ± 25.7 ng/ml was detected applying the silica membrane based protocol and 8.5 ± 7.5 ng/ml using the MBD-approach, both values strongly dependent on the serum sample preparation methods used. In contrast to malignant and benign tumor serum samples, cell free DNA concentrations were significantly elevated in sera of metastasizing breast cancer patients. Technical evaluation revealed that serum upon MBD-based DNA isolation is suitable for protein-array analyses when data are consistent to untreated serum samples. Conclusion MBD affinity purification allows DNA isolations under native conditions retaining the protein function, thus for example enabling combined analyses of DNA methylation and autoantigene-profiles from the same serum sample and thereby improving minimal invasive diagnostics.

  5. Erythrocyte-bound apolipoprotein B in relation to atherosclerosis, serum lipids and ABO blood group.

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    Boudewijn Klop

    Full Text Available INTRODUCTION: Erythrocytes carry apolipoprotein B on their membrane, but the determining factors of erythrocyte-bound apolipoprotein B (ery-apoB are unknown. We aimed to explore the determinants of ery-apoB to gain more insight into potential mechanisms. METHODS: Subjects with and without CVD were included (N = 398. Ery-apoB was measured on fresh whole blood samples using flow cytometry. Subjects with ery-apoB levels ≤ 0.20 a.u. were considered deficient. Carotid intima media thickness (CIMT was determined as a measure of (subclinical atherosclerosis. RESULTS: Mean ery-apoB value was 23.2% lower in subjects with increased CIMT (0.80 ± 0.09 mm, N = 140 compared to subjects with a normal CIMT (0.57 ± 0.08 mm, N = 258 (P = 0.007, adjusted P<0.001. CIMT and ery-apoB were inversely correlated (Spearman's r: -0.116, P = 0.021. A total of 55 subjects (13.6% were considered ery-apoB deficient, which was associated with a medical history of CVD (OR: 1.86, 95% CI 1.04-3.33; adjusted OR: 1.55; 95% CI 0.85-2.82. Discontinuation of statins in 54 subjects did not influence ery-apoB values despite a 58.4% increase in serum apolipoprotein B. Subjects with blood group O had significantly higher ery-apoB values (1.56 ± 0.94 a.u. when compared to subjects with blood group A (0.89 ± 1.15 a.u, blood group B (0.73 ± 0.1.12 a.u. or blood group AB (0.69 ± 0.69 a.u. (P-ANOVA = 0.002. CONCLUSION: Absence or very low values of ery-apoB are associated with clinical and subclinical atherosclerosis. While serum apolipoprotein B is not associated with ery-apoB, the ABO blood group seems to be a significant determinant.

  6. Effect of exercise on Special Aviation Gymnastics Instruments on blood serum levels of selected biochemical indices in cadets

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    Zbigniew Wochyński

    2014-03-01

    Full Text Available Introduction. Aim of this study was the training effect evaluation on the Special Aviation Gymnastics Instruments (SAGI on blood metallothionein (MT, zinc (Zn, copper (Cu, protein, neuron-specific enolase (NSE, and physical fitness in the examined cadets. Material and methods. The study comprised 55 cadets, aged 20, divided into two groups: examined group A (N=41 and control group B (N=14. In both groups, blood material was collected twice, i.e. before (baseline and after training (series I, during (series II, and after completion of training on the SAGI (Series III. Blood serum MT, Zn, Cu, protein, and NSE were assayed with commercially available kits. Physical fitness was assessed with commonly used fitness tests. Results. A significant decrease in serum MT was noted in both groups in all three series of assays after training, except group B in series II. NSE significantly increased in group A in series II after training. NSE activity increased significantly in group B in series I and III. In both groups, a significant decrease in blood serum Zn was noted after training in series I and II. Serum Cu significantly decreased in group A in all three series of assays. Blood serum protein significantly decreased in group A in series III. In series II, blood serum protein increased significantly in both groups. The remaining values were not changed significantly. Conclusions. Training intensity on SAGI lowered serum MT levels after training in comparison with the control group. This might be associated with Zn, Cu, and protein metabolism.

  7. Associations between Serum Sex Hormone Concentrations and Whole Blood Gene Expression Profiles in the General Population.

    Directory of Open Access Journals (Sweden)

    Robin Haring

    Full Text Available Despite observational evidence from epidemiological and clinical studies associating sex hormones with various cardiometabolic risk factors or diseases, pathophysiological explanations are sparse to date. To reveal putative functional insights, we analyzed associations between sex hormone levels and whole blood gene expression profiles.We used data of 991 individuals from the population-based Study of Health in Pomerania (SHIP-TREND with whole blood gene expression levels determined by array-based transcriptional profiling and serum concentrations of total testosterone (TT, sex hormone-binding globulin (SHBG, free testosterone (free T, dehydroepiandrosterone sulfate (DHEAS, androstenedione (AD, estradiol (E2, and estrone (E1 measured by liquid chromatography-tandem mass spectrometry (LC-MS/MS and immunoassay. Associations between sex hormone concentrations and gene expression profiles were analyzed using sex-specific regression models adjusted for age, body mass index, and technical covariables.In men, positive correlations were detected between AD and DDIT4 mRNA levels, as well as between SHBG and the mRNA levels of RPIA, RIOK3, GYPB, BPGM, and RAB2B. No additional significant associations were observed.Besides the associations between AD and DDIT4 expression and SHBG and the transcript levels of RPIA, RIOK3, GYPB, BPGM, and RAB2B, the present study did not indicate any association between sex hormone concentrations and whole blood gene expression profiles in men and women from the general population.

  8. Fatigue, serum cytokine levels, and blood cell counts during radiotherapy of patients with breast cancer

    International Nuclear Information System (INIS)

    Purpose: To assess the level of fatigue during the course of adjuvant radiotherapy (RT) of breast cancer patients and its relation to anxiety, depression, serum cytokines, and blood count levels. Methods and Materials: Forty-one patients who received adjuvant RT after breast-conserving surgery were prospectively studied. All patients underwent RT without concomitant chemotherapy. Patients rated their fatigue with two standardized self-assessment instruments, the Fatigue Assessment Questionnaire and a visual analog scale on fatigue intensity, before RT, during weeks 1-5 of RT, and 2 months after RT completion. In addition, the anxiety and depression levels were assessed with the Hospital Anxiety and Depression Scale. A differential blood cell count and the serum levels of the cytokines interleukin (IL)-1β, IL-6, and tumor necrosis factor-α were determined in parallel to the fatigue assessments. Results: Fatigue intensity as assessed with the visual analog scale increased (p<0.001) until treatment week 4 and remained elevated until week 5. Two months after RT, the values had fallen to the pretreatment levels. Fatigue measured with the Fatigue Assessment Questionnaire did not increase significantly during treatment, but the subscores on physical (p=0.035) and cognitive (p=0.015) fatigue were elevated during treatment weeks 4 and 5. Affective fatigue did not change significantly. Anxiety, as rated with the Hospital Anxiety and Depression Scale, declined during RT (p=0.002), but the Hospital Anxiety and Depression Scale depression score did not change significantly. IL-1β, IL-6, and tumor necrosis factor-α levels did not change during therapy and did not correlate with fatigue. Peripheral blood cell levels declined significantly during therapy and were still low 2 months after treatment. Until treatment week 5, lymphocytes were reduced to almost 50% of their initial values. Hemoglobin levels did not correlate with fatigue. Conclusions: We observed an increase in

  9. Blood serum free amino acids pattern in newborn calves on colostral diet and orally treated with zeolite

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    Gvozdić Dragan

    2010-01-01

    Full Text Available Oral zeolite treatement effects on the blood serum free amino acid pattern in newborn calves was investigated. The total number of 30 newborn Holstein calves of both sexes, weighting 35±3 kg (mean±SD, were immediately after parturition separated from their dams and placed in individual pens. Calves were divided in two experimental groups, 15 calves each. All calves were bottle-fed twice/ day (1.5 L/meal during the first 48 hours after delivery, in 12 hour intervals, with their mother's first (during 24 hours postpartum or second colostrum (at 24-48 hours postpartum, starting two hours after delivery. Zeolite suspension (20 mL, 25% suspension in distilled water was added to every meal for treated calves. Colostrum samples were collected from six cows at 0-12h and 24h after delivery. Total and colostral whey protein concentrations were determined using the colorimetric method. Blood samples were taken from the jugular vein of calves at 6, 16, 30 and 40 hours after birth. After spontaneous coagulation at room temperature blood serum was separated and stored at -20°C until analyzed. Total protein concentration was determined by the colorimetric method. Blood serum immunoglobulin G (IgG concentrations were determined using single radial immunodiffusion (sRID plates. Pooled blood serum free amino acids (aspartic acid - Asp, glutamic acid - Glu, serine - Ser, histidine - His, glycine - Gly, threonine - Thr, alanine - Ala, proline - Pro, tyrosine - Tyr, arginine - Arg, valine - Val, methionine - Met, Leucine - Leu, Isoleucine - Ile, phenylalanine - Phe were identified and quantified using high-performance liquid chromatography (HPLC, GBC Australia. Total and colostral whey protein concentrations were significantly higher in the first colostrum and decreased between 50-75% at 24-48 hours later on. Mean blood serum IgG concentration was significantly increased at 6 and 16 hours in the treated calves (26±7:20±5 and 55±15:42±13 g/L, p<0.05. Blood

  10. IgM AUTOANTIBODIES TO DNA IN BLOOD SERUM OF THE PATIENTS WITH HEMORRHAGIC FEVER WITH RENAL SYNDROME

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    D. G. Ishmukhametova

    2010-01-01

    Full Text Available Levels of IgM autoantibodies (AAbs to native (double-stranded and denaturated (single-stranded DNA were studied in blood serum of sixty patients with hemorrhagic fever with renal syndrome (HFRS and twenty-five healthy persons, using an ELISA technique. The median levels of IgM AAbs to double-stranded DNA in blood serum of healthy persons and HFRS patients corresponded to 0.41 and 0.53 arbitrary units, respectively. Thus, the difference between the samples from HFRS and healthy persons proved to be non-significant. The median level of IgM AAbs to single-stranded DNA in blood sera of HFRS patients (0.71 arbitrary units did significantly exceed serum values of healthy persons (0.57 arbitrary units. A probable involvement of IgM AAbs into regulation of IgG AAbs' production during virus-induced activation of autoimmune events in HFRS patients is discussed.

  11. Slurry sampling in serum blood for mercury determination by CV-AFS

    International Nuclear Information System (INIS)

    The heavy metal mercury (Hg) is a neurotoxin known to have a serious health impact even at relatively low concentrations. A slurry method was developed for the sensitive and precise determination of mercury in human serum blood samples by cold vapor generation coupled to atomic fluorescence spectrometry (CV-AFS). All variables related to the slurry formation were studied. The optimal hydrochloric concentration and tin(II) chloride concentration for CV generation were evaluated. Calibration within the range 0.1-10 μg L-1 Hg was performed with the standard addition method, and compared with an external calibration. Additionally, the reliability of the results obtained was evaluated by analyzing mercury in the same samples, but submitted to microwave-assisted digestion method. The limit of detection was calculated as 25 ng L-1 and the relative standard deviation was 3.9% at levels around of 0.4 μg L-1 Hg

  12. Study of AFP level in the blood serum of the 2223 normal pregnant women

    International Nuclear Information System (INIS)

    The authors have reported AFP level in the blood serum of 2223 normal pregnant women in different semesters by RIA method. All the data were made by statistical analysis. The result shows that the AFP level is normal during the first 12 weeks of pregnancy. Then, it gradually increases from the 13th week and reaches its maximum (184.6 +- 74.6 μg/l) at 32nd week. Later on it tends to decrease slowly, but still remains over 100 μg/l in parturition period. Therefore, it is suggested that AFP in pregnant women has substantial difference and regularity between different semesters but without any significant correlation with their ages

  13. Isotopes and concentrations of Zn in human blood and serum by ICP-MS

    International Nuclear Information System (INIS)

    The double focusing IMS-Plasmax2 is used at different resolving powers, for the separation between zinc isotopes and the interfering ions. NIST standard samples of zinc in the single and multi-elements are used in the analysis. Variation of different Zn isotopic abundances and their interfering ions are studied as a function of concentrations at low and high resolutions. The precision and the accuracy of the data are calculated. Blood and serum samples in human are used as an application for this method in the biological samples. The obtained results are compared with that obtained by using flame atomic absorption spectrometer and it is found in the same range of concentration

  14. Optical properties of solutions consisting of albumin and γ-globulin molecules in different ratio modeling blood serum

    Science.gov (United States)

    Petrova, G. P.; Boiko, A. V.; Fedorova, K. V.; Sergeeva, I. A.; Sokol, N. V.; Tichonova, T. N.

    2009-06-01

    Rayleigh-Debye laser light scattering (RDLS) was used for study of the alterations of physical properties of albumin and γ- globulin molecules water solved in different ratios as a model system of blood serum. By using a portable optical device we simultaneously measured a number parameters of protein solutions such as the effective mass, coefficient of intermolecular interaction, diffusion coefficients of the components, etc. The results of the investigations for model systems were compared with the data obtained for blood serum of healthy and oncology patients.

  15. [Distribution of immunoglobulin isotypes and Salmonella antibodies in blood serum and meat juice of pigs].

    Science.gov (United States)

    Steinbach, Günter; Methner, Ullrich; Meyer, Horst

    2003-01-01

    Using the close linear regression between the logarithm of the dilution degree of a sample and the logarithm of the extinction measured in an ELISA both the relative concentrations of immunoglobulines of the isotypes IgG, IgM and IgA and of the LPS antibodies against S. Typhimurium of the different isotypes in blood sera and meat juice of 15 slaughtered pigs were detected and compared. Furthermore the total concentration of antibodies against LPS of S. Typhimurium according to the "meat juice ELISA" were compared. Distribution of immunoglobulines between serum and meat juice revealed individual differences between the animals as well as between the different immunoglobulin-isotypes. Within the same isotype the ratio of the concentrations of anti-LPS Salmonella Typhimurium antibodies between serum and meat juice was significantly closer than relating the whole of immunoglobulines of the referred isotype. In order to detect pig herds with a high level of Salmonella exposure a comparison of the 1:30 diluted meat juice samples with the 1:400 diluted blood sera is justified, however, for detailed epidemiological or scientific studies there is a need to consider the existing differences between the immunoglobuline-isotypes as well as between the specificity of antibodies and of total immunoglobulines. While the concentration of Salmonella antibodies of the isotypes IgG1, IgG2 and IgA showed a clear and statistically significant correlation between both one below the other and with the total amount of Salmonella antibodies, this connection could not be established for the total amount of immunoglobulines of different isotypes and the IgM-antibodies. PMID:12894680

  16. Cu isotopic signature in blood serum of liver transplant patients: a follow-up study

    Science.gov (United States)

    Lauwens, Sara; Costas-Rodríguez, Marta; van Vlierberghe, Hans; Vanhaecke, Frank

    2016-07-01

    End-stage liver disease (ESLD) is life-threatening and liver transplantation (LTx) is the definitive treatment with good outcomes. Given the essential role of hepatocytes in Cu homeostasis, the potential of the serum Cu isotopic composition for monitoring a patient’s condition post-LTx was evaluated. For this purpose, high-precision Cu isotopic analysis of blood serum of ESLD patients pre- and post-LTx was accomplished via multi-collector ICP-mass spectrometry (MC-ICP-MS). The Cu isotopic composition of the ESLD patients was fractionated in favour of the lighter isotope (by about ‑0.50‰). Post-LTx, a generalized normalization of the Cu isotopic composition was observed for the patients with normal liver function, while it remained light when this condition was not reached. A strong decrease in the δ65Cu value a longer term post-LTx seems to indicate the recurrence of liver failure or cancer. The observed trend in favour of the heavier Cu isotopic composition post-LTx seems to be related with the restored biosynthetic capacity of the liver, the restored hepatic metabolism and/or the restored biliary secretion pathways. Thus, Cu isotopic analysis could be a valuable tool for the follow-up of liver transplant patients and for establishing the potential recurrence of liver failure.

  17. Cu isotopic signature in blood serum of liver transplant patients: a follow-up study

    Science.gov (United States)

    Lauwens, Sara; Costas-Rodríguez, Marta; Van Vlierberghe, Hans; Vanhaecke, Frank

    2016-01-01

    End-stage liver disease (ESLD) is life-threatening and liver transplantation (LTx) is the definitive treatment with good outcomes. Given the essential role of hepatocytes in Cu homeostasis, the potential of the serum Cu isotopic composition for monitoring a patient’s condition post-LTx was evaluated. For this purpose, high-precision Cu isotopic analysis of blood serum of ESLD patients pre- and post-LTx was accomplished via multi-collector ICP-mass spectrometry (MC-ICP-MS). The Cu isotopic composition of the ESLD patients was fractionated in favour of the lighter isotope (by about −0.50‰). Post-LTx, a generalized normalization of the Cu isotopic composition was observed for the patients with normal liver function, while it remained light when this condition was not reached. A strong decrease in the δ65Cu value a longer term post-LTx seems to indicate the recurrence of liver failure or cancer. The observed trend in favour of the heavier Cu isotopic composition post-LTx seems to be related with the restored biosynthetic capacity of the liver, the restored hepatic metabolism and/or the restored biliary secretion pathways. Thus, Cu isotopic analysis could be a valuable tool for the follow-up of liver transplant patients and for establishing the potential recurrence of liver failure. PMID:27468898

  18. Umbilical cord blood serum therapy for the management of persistent corneal epithelial defects

    Institute of Scientific and Technical Information of China (English)

    Elif; Erdem; Meltem; Yagmur; Inan; Harbiyeli; Hande; Taylan-Sekeroglu; Reha; Ersoz

    2014-01-01

    AIM:Toevaluatetheroleofumbilical cord blood serum(CBS) therapy in cases with persistent corneal epithelial defects(PED).METHODS:Sixteen eyes of 14 patients with PED who were resistant to conventional treatment were treated with 20% umbilical cord serum eye drops. Patients were followed-up weekly until epithelization was complete.The collected data included the grade of corneal lesion(Grade I: epithelial defect +superficial vascularization,Grade II: epithelial defect +stromal edema, Grade III:corneal ulcer +stromal melting), the size of epithelial defect(pretreatment, 7th, 14 thand 21stdays of treatment),and follow-up time was evaluated retrospectively.RESULTS:The mean size of epithelial defect on two perpendicular axes was 5.2×4.6-mm2(range: 2.5-8 mm×2.2-9 mm2). Mean duration of treatment was 8.3 ±5wk.CBS therapy was effective in 12 eyes(75%) and ineffective in 4 eyes(25%). The epithelial defects in 4ineffective eyes were healed with amniotic membrane transplantation and tarsorrhaphy. The rate of complete healing was 12.5% by 7d, 25% by 14 d, and 75% by 21 d.The healing time was prolonged in Grade III eyes in comparison to eyes in Grade I or Grade II.CONCLUSION:The results of the current study indicated the safety effectiveness of CBS drops in the management of PED. The grade of disease seems have a role on the healing time.

  19. Correlation of hepatitis C RNA and serum alanine aminotransferase in hepatitis B and C seronegative healthy blood donors

    OpenAIRE

    Ali Natasha; Moiz Bushra; Moatter Tariq; Ahmed Shiraz; Adil Salman; Khurshid Mohammad

    2010-01-01

    Introduction: Historically, serum alanine transaminase (ALT) has been used as a surrogate marker in the detection of hepatitis viruses in blood donors. With the availability of newer sensitive technologies for the detection of seroconversion, the value of ALT becomes questionable but continues to be used for this purpose with subsequent discarding of ALT elevated blood units. Objective: The present study aims to evaluate the significance and cost effectiveness of ALT as a surrogate marker for...

  20. The Effect of Different Types of Musculoskeletal Injuries on Blood Concentration of Serum Amyloid A in Thoroughbred Racehorses

    OpenAIRE

    Turło, Agnieszka; Cywińska, Anna; Czopowicz, Michał; Witkowski, Lucjan; Niedźwiedź, Artur; Słowikowska, Malwina; Borowicz, Hieronim; Jaśkiewicz, Anna; Winnicka, Anna

    2015-01-01

    Background Training-induced muscle, skeletal and joint trauma may result in acute phase response reflected by the changes in the blood concentration of serum amyloid A (SAA) in racehorses. It remains yet unclear if such systemic reaction could be triggered by sport injuries and what is the impact of different types of musculoskeletal trauma on SAA concentrations in racehorses. This study aimed to determine changes in the SAA blood concentration in racehorses with different types of injuries o...

  1. Diagnostic prediction of renal failure from blood serum analysis by FTIR spectrometry and chemometrics

    Science.gov (United States)

    Khanmohammadi, Mohammdreza; Ghasemi, Keyvan; Garmarudi, Amir Bagheri; Ramin, Mehdi

    2015-02-01

    A new diagnostic approach based on Attenuated Total Reflectance-Fourier Transform Infrared (ATR-FTIR) spectrometry and classification algorithm has been introduced which provides a rapid, reliable, and easy way to perform blood test for the diagnosis of renal failure. Blood serum samples from 35 renal failure patients and 40 healthy persons were analyzed by ATR-FTIR spectrometry. The resulting data was processed by Quadratic Discriminant Analysis (QDA) and QDA combined with simple filtered method. Spectroscopic studies were performed in 900-2000 cm-1 spectral region with 3.85 cm-1 data space. Results showed 93.33% and 100% of accuracy for QDA and filter-QDA models, respectively. In the first step, 30 samples were applied to construct the model. In order to modify the capability of QDA in prediction of test samples, filter-based feature selection methods were applied. It was found that the filtered spectra coupled with QDA could correctly predict the test samples in most of the cases.

  2. Serum γ-glutamyltransferase, alanine aminotransferase, and aspartate aminotransferase activity in Iranian healthy blood donor men

    Institute of Scientific and Technical Information of China (English)

    Hossein Khedmat; Nasrin Zarei; Farahnaz Fallahian; Hassan Abolghasemi; Bashir Hajibeigi; Zohre Attarchi; Farshid Alaeddini; Mohammad Taghi Holisaz; Masoumeh Pourali; Shahin Sharifi

    2007-01-01

    AIM: To determine serum γ-glutamyltransferase (GGT), alanine aminotransferase (ALT), and aspartate aminotransferase (AST) activity, and to assess their correlation with demographic and clinical findings in healthy blood donors.METHODS: This cross-sectional study was performed in 934 male blood donors, aged 18 to 68 years, who consecutively attended Tehran blood transfusion service in 2006. All participants were seronegative for HBV or HCV infections, non alcohol users, and all underwent a standard interview and anthropometric tests. Clinical and biochemical parameters including AST, ALT, and GGT activities were determined. Patients taking drugs known to cause hepatic fat deposition were excluded. For AST, ALT, and GGT variables, we used 33.33 and 66.66 percentiles, so that each of them was divided into three tertiles.RESULTS: Mean AST, ALT, and GGT activities were 25.26 ± 12.58 U/L (normal range 5-35 U/L), 33.13 ± 22.98 (normal range 5-35 U/L), and 25.11 ± 18.32 (normal range 6-37 U/L), respectively. By univariate analyses, there were significant associations between increasing AST, ALT, or GGT tertiles and age, body weight, body mass index, and waist and hip circumferences (P < 0.05). By multiple linear regression analyses, ALT was found to be positively correlated with dyslipidemia (B = 6.988, P = 0.038), whereas ALT and AST were negatively correlated with age. AST, ALT, and GGT levels had positive correlation with family history of liver disease (B = 15.763, P < 0.001), (B = 32.345, P < 0.001), (B =24.415, P < 0.001), respectively.CONCLUSION: Although we did not determine the cutoffs of the upper normal limits for AST, ALT, and GGT levels, we would suggest screening asymptomatic patients with dyslipidemia and also subjects with a family history of liver disease.

  3. Immunoelectrophoresis - blood

    Science.gov (United States)

    IEP - serum; Immunoglobulin electrophoresis - blood; Gamma globulin electrophoresis; Serum immunoglobulin electrophoresis ... A blood sample is needed. For information on how this is done, see: Venipuncture

  4. Graphite furnace atomic absorption spectrometry as a routine method for the quantification of beryllium in blood and serum

    Directory of Open Access Journals (Sweden)

    Brousseau Pauline

    2008-07-01

    Full Text Available Abstract Background A routine method for the quantification of beryllium in biological fluids is essential for the development of a chelation therapy for Chronic Beryllium Disease (CBD. We describe a procedure for the direct determination of beryllium in undigested micro quantities of human blood and serum using graphite furnace atomic absorption spectrometry. Blood and serum samples are prepared respectively by a simple 8-fold and 5-fold dilution with a Nash Reagent. Three experimental setups are compared: using no modifier, using magnesium nitrate and using palladium/citric acid as chemical modifiers. Results In serum, both modifiers did not improve the method sensitivity, the optimal pyrolysis and atomization temperatures are 1000°C and 2900°C, respectively. In blood, 6 μg of magnesium nitrate was found to improve the method sensitivity. The optimal pyrolysis and atomization temperatures were 800°C and 2800°C respectively. Conclusion In serum, the method detection limit was 2 ng l-1, the characteristic mass was 0.22 (± 0.07 pg and the accuracy ranged from 95 to 100%. In blood, the detection limit was 7 ng l-1, the characteristic mass was 0.20 (± 0.02 pg and the accuracy ranged from 99 to 101%.

  5. Studies on folate binding and a radioassay for serum and whole blood folate using goat milk as binding agent

    International Nuclear Information System (INIS)

    Preparations of cow, goat, buffalo, and human milk in addition to pig plasma were tested for folate binding properties. Of these, only pig plasma and goat milk showed sufficient binding to enable use as binding agents in a radioassay for serum and whole blood folate. The binding of folate by cow mild preparations in particular was found to be very poor. (orig.)

  6. Arterial blood pressure but not serum albumin concentration correlates with ADC ratio values in pediatric posterior reversible encephalopathy syndrome

    Energy Technology Data Exchange (ETDEWEB)

    Furtado, Andre; Zuccoli, Giulio [Section of Neuroradiology Children' s Hospital of Pittsburgh of UPMC, Department of Radiology, Pittsburgh, PA (United States); Hsu, Ariel [University of Pittsburgh Medical Center, Department of Radiology, Pittsburgh, PA (United States); La Colla, Luca [University of Parma, Department of Anesthesiology, Parma (Italy)

    2015-07-15

    Posterior reversible encephalopathy syndrome (PRES) is a clinical-radiological entity affecting both adults and children characterized by neurotoxicity often in setting of hypertension coupled with distinct brain magnetic resonance imaging features. Decreased serum albumin level has been suggested to correlate with the presence of vasogenic brain edema in adult PRES. Serum albumin has thus been hypothesized to protect against neurotoxicity in PRES by reducing vasogenic brain edema through its role in maintaining plasma osmotic pressure and endothelial integrity. The purpose of our study was to investigate if such correlation between decreased serum albumin level and PRES-related vasogenic edema could be found in children. We conducted a retrospective study of 25 pediatric patients diagnosed with PRES. Underlying clinical conditions, presenting symptoms, blood pressures, and serum albumin levels at onset of symptoms were collected. Brain MR imaging studies were reviewed. We used a quantitative method to evaluate the degree of vasogenic edema by measuring apparent diffusion coefficient (ADC) values of the T2-FLAIR hyperintense brain lesions. No significant correlation was found between serum albumin level and degree of PRES-related vasogenic edema. A significant correlation was found between elevated blood pressure and degree of vasogenic edema in the temporal lobes (p = 0.02 and 0.04, respectively) but not in the other cerebral lobes or cerebellum. Our initial results suggest blood pressure, not serum albumin level, as a main biomarker for brain edema in children with PRES. Thus, our study does not suggest a protective role of serum albumin against PRES-related neurotoxicity in children. (orig.)

  7. Arterial blood pressure but not serum albumin concentration correlates with ADC ratio values in pediatric posterior reversible encephalopathy syndrome

    International Nuclear Information System (INIS)

    Posterior reversible encephalopathy syndrome (PRES) is a clinical-radiological entity affecting both adults and children characterized by neurotoxicity often in setting of hypertension coupled with distinct brain magnetic resonance imaging features. Decreased serum albumin level has been suggested to correlate with the presence of vasogenic brain edema in adult PRES. Serum albumin has thus been hypothesized to protect against neurotoxicity in PRES by reducing vasogenic brain edema through its role in maintaining plasma osmotic pressure and endothelial integrity. The purpose of our study was to investigate if such correlation between decreased serum albumin level and PRES-related vasogenic edema could be found in children. We conducted a retrospective study of 25 pediatric patients diagnosed with PRES. Underlying clinical conditions, presenting symptoms, blood pressures, and serum albumin levels at onset of symptoms were collected. Brain MR imaging studies were reviewed. We used a quantitative method to evaluate the degree of vasogenic edema by measuring apparent diffusion coefficient (ADC) values of the T2-FLAIR hyperintense brain lesions. No significant correlation was found between serum albumin level and degree of PRES-related vasogenic edema. A significant correlation was found between elevated blood pressure and degree of vasogenic edema in the temporal lobes (p = 0.02 and 0.04, respectively) but not in the other cerebral lobes or cerebellum. Our initial results suggest blood pressure, not serum albumin level, as a main biomarker for brain edema in children with PRES. Thus, our study does not suggest a protective role of serum albumin against PRES-related neurotoxicity in children. (orig.)

  8. Related research of blood glucose, blood lipid and serum cystatin C and lower-extremity arterial disease in type 2 diabetic mellitus

    Institute of Scientific and Technical Information of China (English)

    Juan Zeng; Jie Ou; Yun-Mei He; Chun-Yu Cai

    2016-01-01

    Objective:To explore the relationship between levels of blood glucose, blood lipid and serum cystatin C and lower-extremity arterial disease in type 2 diabetic mellitus so as to provide the basis for the prevention and treatment of the disease.Methods:A total of 240 cases of patients with type 2 diabetic mellitus receiving physical examinations in our hospital from March 2014 to March 2015 were selected and divided into three groups. The control group was consisted of type 2 diabetes patients without lower-extremity arterial disease, the observation group 1 included type 2 diabetes patients with mild or moderate lower-extremity arterial disease, and the observation group 2 was formed by type 2 diabetes patients with severe lower-extremity arterial disease. Each group contained 80 cases. Then, the heights, weights, waist and hip circumferences of patients in the three groups were determined and the body mass index (BMI) and waist to hip ratio (WHR) were calculated; the fasting blood glucose (FBG), fasting insulin (FINS), insulin resistance index (HOMA-IR), 2 h postprandial blood glucose (2 h PG) and glycosylated hemoglobin (HBA1c) in two groups were detected; and the levels of serum total cholesterol(TC), triglyceride (TG), low density lipoprotein cholesterol (LDL-C), high density lipoprotein cholesterol (HDL-C), apolipoprotein A1 (apoA1), apolipoprotein B (apoB) and cystatin C (CysC) in two groups were also tested.Results:Compared with the control group, the WHR, FBG, HOMA-IR, 2 h PG, HBA1c, LDL-C, apoB and CysC all increased significantly, and the differences were statistically significant. Besides, there were statistically significances existing between the observation groups 1 and 2. What’s more, the serum levels of TC, HDL-C, TG and apoA1 in the observation groups were not significantly different from those of the control group.Conclusions: There is a close relationship between blood glucose, blood lipid, serum cystatin C level and lower-extremity arterial

  9. Clinical significance of determination of changes of serum ferritin, MMP-2 and MMP-9 levels and after transfusion of red blood cells in patients with chronic nephritis

    International Nuclear Information System (INIS)

    Objective: To explore the changes of serum Ferritin, MMP-2 and MMP-9 contents after transfusion of red blood cells in patients with chronic nephritis. Methods: Serum Ferritin (with RIA) and serum MMP-2, MMP-9 (with ELISA) levels were measured in 32 patients with chronic nephritis both before and after a course of transfusion of red blood cells and 35 controls. Results: Before transfusion, the serum Ferritin, MMP-9 levels in the patients were significantly lower than those in controls (P 0.05). Conclusion: Determination of serum Ferritin, MMP-2 and MMP-9 levels is clinically useful for management of patients with chronic nephritis. (authors)

  10. Comparison of extraction and quantification methods of perfluorinated compounds in human plasma, serum, and whole blood

    International Nuclear Information System (INIS)

    Perfluorinated compounds are ubiquitous in the environment and have been reported to occur in human blood. Accurate risk assessments require accurate measurements of exposures, but identification and quantification of PFCs in biological matrices can be affected by both ion suppression and enhancement in liquid chromatography-tandem mass spectrometry techniques (LC/MS-MS). A study was conducted to quantify potential biases in LC/MS-MS quantification methods. Using isotopically labeled perfluorooctanoic acid ([13C2]-PFOA), perfluorononanoic acid ([13C2]-PFNA), and ammonium perfluorooctanesulfonate ([18O2]-PFOS) spiked tissues, ion-pairing extraction, solid-phase extraction, and protein precipitation sample preparation techniques were compared. Analytical accuracy was assessed using both solvent calibration and matrix-matched calibration for quantification. Data accuracy and precision of 100 ± 15% was demonstrated in both human sera and plasma for all three sample preparation techniques when matrix-matched calibration was used in quantification. In contrast, quantification of ion-pairing extraction data using solvent calibration in combination with a surrogate internal standard resulted in significant analytical biases for all target analytes. The accuracy of results, based on solvent calibration was highly variable and dependent on the serum and plasma matrices, the specific target analyte [13C2]-PFOA, [13C2]-PFNA, or [18O2]-PFOS, the target analyte concentration, the LC/MS-MS instrumentation used in data generation, and the specific surrogate internal standard used in quantification. These results suggest that concentrations of PFCs reported for human blood using surrogate internal standards in combination with external solvent calibration can be inaccurate unless biases are accounted for in data quantification

  11. DETERMINATION OF UREA IN BLOOD SERUM BY CONDUCTOMETRIC BIOSENSOR Screen Printed Carbon Electrode (SPCE – NATA DE COCO

    Directory of Open Access Journals (Sweden)

    Sendy Kurniawan

    2011-11-01

    Full Text Available Urea is byproduct of protein degradation in normal serum about 10.7 – 42.8 mg/dL. Conductometric biosensor for determination urea in blood serum based on hydrolysis reaction of urea by urease produce ammonia (NH3 and carbon dioxide (CO2 which will be ionized in water. The research, optimum condition of urease mass, nata de coco membrane thickness, and pH urea solution were studied for determination performance of biosensor while the biosensor was applied for blood serum sample. The biosensor was made from Screen Printed Carbon Electrode (SPCE which coated nata de coco immobilized urease. Accordingly, various pH (6; 7; 8; 9, urease mass (0.1; 0.5; 1,0; 1.5 µg, and membran thickness (5; 10; 15 µm was studied in detail with range concentrations of urea solution were 0 to 5 ppm in phophate buffer 0.01 M pH 8 and SPCE area 5 mm2. The result showed that the optimum performance in 1 µg urease mass, 5 µm membrane thickness, and pH solution was 8, whereas the sensitivity was 14.8 µS/ppm, detection limit was 0.035 ppm, and range concentrations of urea were 0.035 to 0.400 ppm. The biosensor have 73 to 87 % accuration.when be applied in blood serum sample.

  12. Relationship between blood haemoglobin and serum iron concentrations and heart girth in pre-weaned dairy calves

    Directory of Open Access Journals (Sweden)

    Martina Klinkon

    2010-01-01

    Full Text Available The aim of the research was to study the relation between blood haemoglobin (Hb and serum iron (Fe concentrations and changing of heart girth with age in calves. Blood samples of 66 dairy calves were taken at the age of 3, 5 and 16 weeks (wks. Blood samples were analysed for Hb and serum Fe concentration. At each bleeding, the heart girth was measured. The lowest mean concentration of Hb was measured at the age of five wks. The concentration of serum Fe and heart girth increased with age. Significant positive correlations were found between Fe concentration at 3 wks of age and heart girth of calves at 5wks (r=0.270; P=0.032 and between Fe concentration at 5 wks and heart girth of calves at 5 (r=0.283; P=0.021 and 16 wks (r=0.284; P=0.028. The lowest Hb concentration recorded at 5 wks, might suggest that this is the most critical period for developing anaemia in calves. The correlations established between heart girth and Fe concentration at different ages indicate that serum Fe concentration has some influence on changing of heart girth in calves.

  13. Partitioning and levels of neutral organochlorine compounds in human serum, blood cells, and adipose and liver tissue.

    Science.gov (United States)

    Mussalo-Rauhamaa, H

    1991-04-15

    Concentrations of neutral organochlorine compounds (OCs) in blood compartments and adipose tissue were determined in three groups of subjects. In 12 healthy volunteers a positive correlation was found between DDT residue levels in paired serum/adipose tissue samples when the concentrations were calculated on a fat-weight basis (r = 0.74, p less than 0.05); positive correlations were also found for PCB and HCB when the calculations were based on a wet-weight basis (r = 0.68, p less than 0.01; r = 0.69, p less than 0.01). For lindane the correlation coefficient for paired serum/adipose tissue samples was -0.94 (p less than 0.01). The association between adipose tissue and blood cells was weaker than that obtained for serum. These readily obtainable samples are adequate for estimating, or monitoring, the total burden of neutral organochlorines in adipose tissue, especially in cases of low chronic exposure, such as those found in epidemiological studies. In paired liver-adipose tissue samples from 23 autopsy cases, no correlation was found either on a wet- or fat-weight basis. In 131 adults resident in southern and eastern Finland the concentration medians for serum were 1.8, 2.0 and 0.3 ng g-1 wet wt for DDT compounds. PCB and HCB, respectively. This study indicates that monitoring of fat/serum ratios may provide, with tissue concentrations, more information about human exposure to OCs. PMID:1909054

  14. SDS-PAGE Patterns of Blood Serum Proteins in some Species of the Genus Meriones (Mammalia: Rodentia)

    OpenAIRE

    ÇOLAK, Reyhan; Nuri YİĞİT; ÇOLAK, Ercüment

    2002-01-01

    The blood serum proteins of Meriones meridianus, Meriones crassus, Meriones persicus and Meriones tristrami, all found in Turkey, along with domestic Meriones unguiculatus, which originated in Mongolia, were examined for the first time by SDS-PAGE. In the globulin zone, seven to ten bands were identified, one band in the post-albumin and albumin zones and one or two bands in the pre-albumin zone of five species of the genus Meriones. There was no diagnostic difference in the pattern of serum ...

  15. Trace element determination in fingernails, hair and blood serum in patients with Crohn's disease using neutron activation analysis

    International Nuclear Information System (INIS)

    The determination of trace elements and electrolyte concentrations in blood serum, hair and fingernails of 16 patients with Crohn's disease was carried out by means of instrumental neutron activation analysis. In the serum a significant decline in the zinc content could be registered, while the remaining trace elements remained in the normal range. The parenteral nutrition also showed a zinc deficiency. There was, however, also an iron deficiency. The studies of the hair and fingernails gave the following results: Rubidium and antimony in the normal range; zinc, selenium, iron and cobalt below normal values. (orig./PW)

  16. Investigations of presence of antibodies against bovine herpesvirus-1 in blood serum of calves prior to colostrum diet

    Directory of Open Access Journals (Sweden)

    Lazić Sava

    2010-01-01

    Full Text Available The paper presents the results of investigations of the presence of the bovine herpesvirus-1 (BHV-1 in samples of blood serum from 106 cows and 107 of their calves (one cow had twins. Blood was sampled from the cows immediately after parturition, and from the calves before feeding on colostrum. The examined cows and their calves originated from 5 herds in which previous investigations had shown infection with the bovine herpesvirus-1. The determination of antibodies against BHV-1 was performed using the method of virus neutralization in culture of MDBK cells with 100 TCID/50 viruses (BHV-1, TN-41 Am. Bio Research, USA. Antibodies against BHV-1 were determined in all blood serum samples of cows and in 16 samples of precolostral blood serums of calves. The antibody titer values in cows ranged from 1:4 to 1:512, and in calves the determined values were from 1:2 to 1:16. The results indicate that cows that are seropositive to BHV-1 can deliver calves seropositive to BHV-1 in about 15% cases. This must be kept in mind in selecting cows for the production of breeding material, in particular bulls for reproduction centers, as well as in making a programme for the immunoprophylaxis of calves against BHV-1. .

  17. The effects of omega-3 on blood pressure and the relationship between serum visfatin level and blood pressure in patients with type II diabetes

    OpenAIRE

    Hosseinzadeh Atar, Mohammad Javad; Hajianfar, Hossein; Bahonar, Ahmad

    2012-01-01

    BACKGROUND Hypertension is a condition normally detected in people with type II diabetes.It eventually leads to cardiovascular diseases in the patient. Visfatin is an adipocytokine which issecreted from adipose tissue and can affect the inflammatory reaction and also serum lipidlevels. Additionally, omega-3 inhibits the accumulation of fat and formation of insulinresistance. The current study tried to investigate the effects of omega-3 on blood pressurecompared to placebo and the relationship...

  18. Detection of IL28B SNP DNA from buccal epithelial cells, small amounts of serum, and dried blood spots.

    Directory of Open Access Journals (Sweden)

    Philippe Halfon

    Full Text Available BACKGROUND & AIMS: Point mutations in the coding region of the interleukin 28 gene (rs12979860 have recently been identified for predicting the outcome of treatment of hepatitis C virus infection. This polymorphism detection was based on whole blood DNA extraction. Alternatively, DNA for genetic diagnosis has been derived from buccal epithelial cells (BEC, dried blood spots (DBS, and genomic DNA from serum. The aim of the study was to investigate the reliability and accuracy of alternative routes of testing for single nucleotide polymorphism allele rs12979860CC. METHODS: Blood, plasma, and sera samples from 200 patients were extracted (400 µL. Buccal smears were tested using an FTA card. To simulate postal delay, we tested the influence of storage at ambient temperature on the different sources of DNA at five time points (baseline, 48 h, 6 days, 9 days, and 12 days. RESULTS: There was 100% concordance between blood, plasma, sera, and BEC, validating the use of DNA extracted from BEC collected on cytology brushes for genetic testing. Genetic variations in HPTR1 gene were detected using smear technique in blood smear (3620 copies as well as in buccal smears (5870 copies. These results are similar to those for whole blood diluted at 1/10. A minimum of 0.04 µL, 4 µL, and 40 µL was necessary to obtain exploitable results respectively for whole blood, sera, and plasma. No significant variation between each time point was observed for the different sources of DNA. IL28B SNPs analysis at these different time points showed the same results using the four sources of DNA. CONCLUSION: We demonstrated that genomic DNA extraction from buccal cells, small amounts of serum, and dried blood spots is an alternative to DNA extracted from peripheral blood cells and is helpful in retrospective and prospective studies for multiple genetic markers, specifically in hard-to-reach individuals.

  19. Relationship and significance of serum leptin with blood insulin and lipid in 6-13 years old obese children

    International Nuclear Information System (INIS)

    To explore relationship and significance of Serum Leptin with BMI, Insulin, triglyceride (TG) and total cholesterol (TC) in obese children aged 6-13 years. Serum Leptin of school-age children 118 (64 male, 54 female; normal non-obese 56 and obese 62) were deter- mined and compared with BMI, Insulin, TG and TC. The results showed that: (1) Each index of obese children was remarkably higher than that of non-obese children (P0.05). (3) Leptin was poritinely corelation with BMI, insulin, TG and TC(P=0.001). Leptin level in serum may varied according to sex, BMI or blood lipid level. It is of great significance in prevention and treatment of obesity to use drug which may improve Leptin receptor effect. (authors)

  20. Effect of Buddhist meditation on serum cortisol and total protein levels, blood pressure, pulse rate, lung volume and reaction time.

    Science.gov (United States)

    Sudsuang, R; Chentanez, V; Veluvan, K

    1991-09-01

    Serum cortisol and total protein levels, blood pressure, heart rate, lung volume, and reaction time were studied in 52 males 20-25 years of age practicing Dhammakaya Buddhist meditation, and in 30 males of the same age group not practicing meditation. It was found that after meditation, serum cortisol levels were significantly reduced, serum total protein level significantly increased, and systolic pressure, diastolic pressure and pulse rate significantly reduced. Vital capacity, tidal volume and maximal voluntary ventilation were significantly lower after meditation than before. There were also significant decreases in reaction time after mediation practice. The percentage decrease in reaction time during meditation was 22%, while in subjects untrained in meditation, the percentage decrease was only 7%. Results from these studies indicate that practising Dhammakaya Buddhist meditation produces biochemical and physiological changes and reduces the reaction time. PMID:1801007

  1. A label-free fluorescent aptasensor for selective and sensitive detection of streptomycin in milk and blood serum.

    Science.gov (United States)

    Taghdisi, Seyed Mohammad; Danesh, Noor Mohammad; Nameghi, Morteza Alinezhad; Ramezani, Mohammad; Abnous, Khalil

    2016-07-15

    Sensitive and fast detection of antibiotic residues in animal derived foods and blood serum is of great interest. In this study a fluorescent aptasensor was designed for selective and sensitive detection of streptomycin (STR) based on Exonuclease III (Exo III), SYBR Gold and aptamer complimentary strand. In the absence of STR, the fluorescence intensity is weak. Upon addition of STR, the aptamer binds to its target, leading to release of complementary strand from aptamer and more protection against Exo III function. Following addition of SYBR Gold, a strong fluorescence intensity is obtained. This aptasensor showed a high selectivity toward STR with a limit of detection (LOD) as low as 54.5 nM. The validity of the procedure and applicability of the aptasensor were successfully assessed by detection of STR in a spiked milk and blood serum without interference from the sample matrix. PMID:26948599

  2. Hormone-metabolic parameters of blood serum at revealing the metabolic syndrome at liquidators on Chernobyl disaster

    International Nuclear Information System (INIS)

    The purpose of research was the definition of the maintenance leptin, other hormones and some metabolic parameters in liquidators blood serum of group 1.1. Under supervision was 30 healthy persons who were not treat to action of radiation-ecological factors, and 154 liquidators. It is established, that in blood serum of liquidators with body mass index > 25 kg/m2 leptin concentration is authentically raised and cortisol concentration is lowered. Following most important results are received: 1) hyperleptinemia and hypo-alpha-cholesterolemia can be markers of a radiating influence available in the past; 2) the strict algorithm of revealing of metabolic syndrome X allows to generate adequate groups of risk of the diseases interfaced with an insulin resistance and an atherosclerosis development; 3) the strict algorithm of metabolic syndrome X revealing allows to define concrete directions of metabolic preventive maintenance and therapy at the persons who have entered into risk-groups of diseases development. (authors)

  3. [Mineral content of blood serum in greenland seal pups during the period of adaptation to the captivity].

    Science.gov (United States)

    Erokhina, I A

    2003-01-01

    Results of investigation of mineral levels in the harp seal pups blood serum in connection with adaptation to the captivity are presented. The contents of copper, zinc, iron, magnesium, cobalt, manganese, sodium, potassium, calcium and phosphorus in the first 18 day of animals keeping in the oceanarium were determined. The causes of the revealed differences in changes of the contents of single elements during the observation term, and also question on duration of fading stress alterations and stabilization of biochemical parameters of marine mammals blood are discussed. PMID:15143524

  4. Study of the Effect of Garlic on Serum Lipids and Blood Glucose Levels in Type 2 Diabetic Patients

    Directory of Open Access Journals (Sweden)

    M Afkhami - Ardekani

    2005-04-01

    Full Text Available Introduction: Hyperlipidemia and diabetes are common risk factors for ischemic heart disease, which is the main cause of mortality in diabetic patients. Strict control of blood glucose and other risk factors in diabetics has led to prevention of complications. Garlic has received particular attention for control of blood glucose and decrease in blood lipid levels. At present, several studies have been carried out in order to prove advantages of garlic. Methods: In this study, effects of garsin (a derivative of garlic present in our country on serum lipids and blood glucose levels in diabetes mellitus type 2 patients was observed. Forty-five type 2 diabetics who had hyperlipidemia were selected. These patients were kept on treatment with 3 tablets of Garsin / day for 4 weeks. Serum lipids and blood glucose levels were measured prior to and at the end of treatment. Results: Relationship between sex and response to treatment in this study was meaningful, such that Gsarsin led to decrease in LDL-C and increase in HDL in females. Conclusion: Therefore, Garsin can be used as an adjunct to treatment in diabetes type 2 patients with hyperlipidemia.

  5. Studies on Some Biophysical Properties of the Serum Protein of Mice blood exposed to an electric field

    International Nuclear Information System (INIS)

    As an indication of the effect of the electric field on each of the dielectric properties and the molecular structure of the serum protein of the mice blood, an electric field of a 6 kv/m strength and 50 Hz frequency was directed to three groups of mice for exposure periods 30, 45 and 60 days respectively, and investigated directly. Another group was exposed to also 60 days, but investigated after 30 days from switching off the electric field for delayed effect studies. The molecular structure of the serum protein was studied by measuring each of the dielectric relaxation and the electric conductivity in the frequency range 0.15 MHz at 4 ± 0.5 degree C and the dielectric increment (Δ), relaxation time (τ) and average molecular radii (τ) were calculated for all groups. The absorption spectra of the extracted protein were also measured in the wavelength range 200 600 nm. Moreover, electrophoresis of enzymes B-esterase, lactate and Malate dehydrogenase extracted from the blood serum of exposed mice were taken by using the gel electrophoresis technique. The results indicated that exposure of the animals to 50 H, 6 kv/m electric field resulted in the decrease of serum protein permittivity values and increase its conductivity a fact that indicates pronounced changes in the molecular structure of total serum protein the exposed mice. In addition, the intensity of the absorption spectral bands of serum protein of exposed mice were found to decrease relative to unexposed mice. Also the enzymes B-esterase and lactate dehydrogenase were slightly affected by exposing to the electric field whereas their number of bands and their intensities changed relative to the unexposed mice but the malate dehydrogenase was not affected

  6. Comparison of blood serum selenium concentrations from elderly population living in Sao Paulo city and literature data

    International Nuclear Information System (INIS)

    Selenium is an essential nutrient of fundamental importance to human biology. This element is a component of the enzyme glutathione peroxidase that avoids the formation of free radicals protecting the organism against the oxidative damage. The Se concentrations in serum vary from one part of the world to another and this fact has been satisfactorily explained as a consequence of different Se intakes of the population. The main objective of this paper was the determination of serum Se concentrations in healthy elderly population included in a program for 'Successful Ageing' of the Hospital das Clinicas, Sao Paulo University Medical School. The blood samples were collected after a 12 h fast from 32 subjects aged 60 to 87 years. The blood was centrifuged after completely clotted and 4.0 mL of serum were freeze.dried for Se determination using neutron activation analysis. The serum samples and Se standard were irradiated for 16 h under a thermal neutron flux of de 5 x 1012 n cm-'2 s-1 at the IEA-R1 nuclear reactor. Se was determined by measuring the gamma activity of 75Se using an HGe detector coupled to a gamma ray spectrometer. The mean value of (0.92±0.07) μmol L-1 was obtained for serum Se concentration. This value is within the data used as reference values in clinical laboratories and those published in literature. Results obtained for certified reference materials NIST 1566b Oyster Tissue and NIST 1577b Bovine Liver showed good precision and agreed with the certified values. The relative standard deviation of the results was lower than 4.8 % and relative error lower than 0.97 %. Detection limit value obtained for serum Se determination was 0.003 μmol L-1. (author)

  7. Biochemical indices of blood serum of cows with acute radiation sickness due to nuclear fission products. [/sup 235/U

    Energy Technology Data Exchange (ETDEWEB)

    Yakovleva, V.P.; Burov, N.I

    1977-01-01

    Fission products of /sup 235/U were given to cows daily for 4 days. The animals developed the intestinal form of acute radiation sickness and did not survive longer than 20 days. Blood samples were taken at intervals and determinations were made on activity of transaminases and alkaline phosphatase, serum carbohydrates, cholesterol, lecithin, total lipids, K, Na, and Fe. Results showed an increase in enzyme activity, increase in carbohydrate levels, decrease in lipids and electrolytes, and weight loss. (HLW)

  8. Determination of total creatine kinase activity in blood serum using an amperometric biosensor based on glucose oxidase and hexokinase.

    Science.gov (United States)

    Kucherenko, I S; Soldatkin, O O; Lagarde, F; Jaffrezic-Renault, N; Dzyadevych, S V; Soldatkin, A P

    2015-11-01

    Creatine kinase (CK: adenosine-5-triphosphate-creatine phosphotransferase) is an important enzyme of muscle cells; the presence of a large amount of the enzyme in blood serum is a biomarker of muscular injuries, such as acute myocardial infarction. This work describes a bi-enzyme (glucose oxidase and hexokinase based) biosensor for rapid and convenient determination of CK activity by measuring the rate of ATP production by this enzyme. Simultaneously the biosensor determines glucose concentration in the sample. Platinum disk electrodes were used as amperometric transducers. Glucose oxidase and hexokinase were co-immobilized via cross-linking with BSA by glutaraldehyde and served as a biorecognition element of the biosensor. The biosensor work at different concentrations of CK substrates (ADP and creatine phosphate) was investigated; optimal concentration of ADP was 1mM, and creatine phosphate - 10 mM. The reproducibility of the biosensor responses to glucose, ATP and CK during a day was tested (relative standard deviation of 15 responses to glucose was 2%, to ATP - 6%, to CK - 7-18% depending on concentration of the CK). Total time of CK analysis was 10 min. The measurements of creatine kinase in blood serum samples were carried out (at 20-fold sample dilution). Twentyfold dilution of serum samples was chosen as optimal for CK determination. The biosensor could distinguish healthy and ill people and evaluate the level of CK increase. Thus, the biosensor can be used as a test-system for CK analysis in blood serum or serve as a component of multibiosensors for determination of important blood substances. Determination of activity of other kinases by the developed biosensor is also possible for research purposes. PMID:26452867

  9. Determination of total creatine kinase activity in blood serum using an amperometric biosensor based on glucose oxidase and hexokinase

    OpenAIRE

    Kucherenko, Ivan S; Soldatkin, Oleksandr O; Lagarde, Florence; Jaffrezic-Renault, Nicole; Dzyadevych, S.V.; Soldatkin, A.P.

    2015-01-01

    International audience Creatine kinase (CK: adenosine-5-triphosphate-creatine phosphotransferase) is an important enzyme of muscle cells; the presence of a large amount of the enzyme in blood serum is a biomarker of muscular injuries, such as acute myocardial infarction. This work describes a bi-enzyme (glucose oxidase and hexokinase based) biosensor for rapid and convenient determination of CK activity by measuring the rate of ATP production by this enzyme. Simultaneously the biosensor de...

  10. Effects of Clove Oil as a Euthanasia Agent on Blood Collection Efficiency and Serum Cortisol Levels in Danio rerio.

    Science.gov (United States)

    Davis, Daniel J; Klug, Jenna; Hankins, Miriam; Doerr, Holly M; Monticelli, Stephanie R; Song, Ava; Gillespie, Catherine H; Bryda, Elizabeth C

    2015-09-01

    Zebrafish are an important laboratory animal model for biomedical research and are increasingly being used for behavioral neuroscience. Tricaine methanesulfonate (MS222) is the standard agent used for euthanasia of zebrafish. However, recent studies of zebrafish behavior suggest that MS222 may be aversive, and clove oil might be a possible alternative. In this study, we compared the effects of MS222 or clove oil as a euthanasia agent in zebrafish on the volume of blood collected and on serum levels of cortisol. Greater amounts of serum could be collected and lower serum levels of cortisol were present in fish euthanized with clove oil compared with equipotent dose of MS222. Euthanasia with clove oil did not blunt the expected elevation of serum cortisol levels elicited by an acute premortem stress. According to our findings, clove oil is a fast-acting agent that minimizes the cortisol response to euthanasia in zebrafish and allows the collection of large volumes of blood postmortem. These results represent a significant refinement in euthanasia methods for zebrafish. PMID:26424256

  11. Characterization of monoclonal gammopathy of undetermined significance by calorimetric analysis of blood serum proteome

    OpenAIRE

    Barceló, Francisca; Cerdà, Joan J.; Gutiérrez, Antonio; Jiménez-Marco, Teresa; Durán, Maria Antònia; Novo, Andrés; Ros, Teresa; Sampol, Antonia; Portugal, José

    2015-01-01

    © 2015 Barceló et al. Monoclonal gammopathy of undetermined significance (MGUS) is a premalignant proliferative disorder thatmay progress to multiplemyeloma, a malignant plasma cell neoplasia.We evaluated differential scanning calorimetry (DSC) as an experimental tool for differentiating serum samples of MGUS patients from healthy individuals. DSC thermograms can be used for monitoring changes in the serum proteome associated with MGUS. MGUS patients showed great variability in serum thermogr...

  12. Characterization of Monoclonal Gammopathy of Undetermined Significance by Calorimetric Analysis of Blood Serum Proteome

    OpenAIRE

    Barceló, Francisca; Cerdà, Joan J.; Gutiérrez, Antonio; Jimenez-Marco, Teresa; Durán, M. Antonia; Novo, Andrés; Ros, Teresa; Sampol, Antonia; Portugal, José

    2015-01-01

    Monoclonal gammopathy of undetermined significance (MGUS) is a premalignant proliferative disorder that may progress to multiple myeloma, a malignant plasma cell neoplasia. We evaluated differential scanning calorimetry (DSC) as an experimental tool for differentiating serum samples of MGUS patients from healthy individuals. DSC thermograms can be used for monitoring changes in the serum proteome associated with MGUS. MGUS patients showed great variability in serum thermogram characteristics,...

  13. Mobile and cordless telephones, serum transthyretin and the blood-cerebrospinal fluid barrier: a cross-sectional study

    Directory of Open Access Journals (Sweden)

    Carlberg Michael

    2009-04-01

    Full Text Available Abstract Background Whether low-intensity radiofrequency radiation damages the blood-brain barrier has long been debated, but little or no consideration has been given to the blood-cerebrospinal fluid barrier. In this cross-sectional study we tested whether long-term and/or short-term use of wireless telephones was associated with changes in the serum transthyretin level, indicating altered transthyretin concentration in the cerebrospinal fluid, possibly reflecting an effect of radiation. Methods One thousand subjects, 500 of each sex aged 18–65 years, were randomly recruited using the population registry. Data on wireless telephone use were assessed by a postal questionnaire and blood samples were analyzed for serum transthyretin concentrations determined by standard immunonephelometric techniques on a BN Prospec® instrument. Results The response rate was 31.4%. Logistic regression of dichotomized TTR serum levels with a cut-point of 0.31 g/l on wireless telephone use yielded increased odds ratios that were statistically not significant. Linear regression of time since first use overall and on the day that blood was withdrawn gave different results for males and females: for men significantly higher serum concentrations of TTR were seen the longer an analogue telephone or a mobile and cordless desktop telephone combined had been used, and in contrast, significantly lower serum levels were seen the longer an UMTS telephone had been used. Adjustment for fractions of use of the different telephone types did not modify the effect for cumulative use or years since first use for mobile telephone and DECT, combined. For women, linear regression gave a significant association for short-term use of mobile and cordless telephones combined, indicating that the sooner blood was withdrawn after the most recent telephone call, the higher the expected transthyretin concentration. Conclusion In this hypothesis-generating descriptive study time since first

  14. Nutrient intake, blood pressure, serum and urinary prostaglandins and serum thromboxane B2 in a controlled trial with a lacto-ovo-vegetarian diet.

    Science.gov (United States)

    Rouse, I L; Beilin, L J; Mahoney, D P; Margetts, B M; Armstrong, B K; Record, S J; Vandongen, R; Barden, A

    1986-04-01

    Fifty-nine healthy omnivores volunteered for a randomized crossover trial with a lacto-ovo-vegetarian (L-O-V) diet. Twenty-one 1-day diet records were kept throughout the project as a means of assessing food and nutrient intakes, and samples of serum and urine were assayed to evaluate change in prostanoid metabolism. While on the L-O-V diet subjects ate more vegetable protein, wholegrain cereals, polyunsaturated oils, fruits and vegetables, and avoided eating meat, fish or poultry. The L-O-V diet contained significantly more polyunsaturated fatty acids, fibre, vitamin C, vitamin E, magnesium, calcium and potassium, and less total protein, saturated fat, monounsaturated fat and vitamin B12 than the control omnivore diet. Changes in nutrient intakes were subjected to principal components analysis to identify dimensions of change in nutrient intakes. Three Factors accounted for 83% of the total variation in dietary intake. Blood pressure changes were significantly and negatively (F = 17.4, P less than 0.001 for systolic; F = 6.09, P = 0.02 for diastolic pressure) related to individual scores for only one Factor--that representing an increase in intake of polyunsaturated fat, fibre, vitamin C, vitamin E, calcium and magnesium, and a fall in intake of protein and vitamin B12. Blood pressure changes were unrelated to change in body weight or sodium intake. Serum and urinary prostanoids were not affected by eating the L-O-V diet. PMID:3011891

  15. CORRELATIONS BETWEEN BLOOD PRESSURE AND BODY WEIGHT, SERUM LEPTIN IN HIGH CALORIE DIET-INDUCED OBESE RATS

    Institute of Scientific and Technical Information of China (English)

    Hu Zhi; Ma Aiqun; Yang Chun; Tian Hongyan

    2006-01-01

    Objective To examine the change of body weight (BW) and blood pressure (BP) in obese rats, clarify relationships between BP and BW and other factors. Methods Male Spraque-Dawley rats were fed either with normal diet (ND) or high calorie diet (HC) for 20 weeks. BW and BP of tail artery were observed biweekly and tetraweekly respectively; serum leptin and fasting insulin (FINS) were detected by enzyme-linked immunoadsordent assay (ELISA) and radioimmunoassay (RIA) respectively. Fasting plasma glucose (FPG) and free fatty acid(FFA) were measured by conventional means. Results BW, abdominal fat weight (AFW), ratio of abdominal fat weight to body weight (RF/W), systolic blood pressure (SBP), diastolic blood pressure (DBP), serum levels of leptin and FINS, FPG, FFA increased in the HD group after 20 weeks diet intervention (P<0.05 or P<0.01). SBP was strongly correlated with BW, leptin, FINS and FFA (P<0.05), DBP was correlated with FFA (r=0.47, P<0.05). In addition, leptin was positively correlated with BW, AFW, RF/W, FINS and FFA (P<0.05 or P<0.01). Conclusion In this study of high calorie-diet induced rats, the gain of BW is accompanied by increased BP. The obese rats have hyperleptinemia, hyperinsulinemia, hyperglycemia and dyslipidemia which may have important effects on the development of obesity-related hypertension. RF/W is the key factor in which affect serum leptin level.

  16. Effect of Oral Contraceptive Pills on the Blood Serum Enzymes and DNA Damage in Lymphocytes Among Users.

    Science.gov (United States)

    Naz, Falaq; Jyoti, Smita; Rahul; Akhtar, Nishat; Siddique, Yasir Hasan

    2016-07-01

    The continuous use of synthetic hormones as contraceptive pill or hormonal replacement therapy among women is increasing day by day. The widespread use of different formulations as oral contraceptives by women throughout their reproductive cycle has given rise to a serious concern for studying the effects of oral contraceptives on enzymatic profile and DNA damage in peripheral blood lymphocytes among users. The present study was carried out on women taking oral contraceptives. The study was based on the questionnaire having the information of reproductive history, fasting, age, health, nature of menstrual cycle, bleeding and other disease. The profile of the blood serum enzymes i.e. alkaline phosphatase (ALP), gamma glutamyl transferase (GGT), lactate dehydrogenase (LDH), aminotransferases (SGOT and SGPT), serum proteins (albumin and globulin) and DNA damage in lymphocytes was studied among users and non-users. The results of the present study suggest that OCs not only effects enzymatic activity but also results in DNA damage that may vary with the duration of using oral contraceptives. A significant increase in LDH, GGT, SGPT, SGOT, globulin and decrease in ALP as well as albumin was found among users as compared to non-users. The observed DNA damage was more in users as compared to non-users. Hormonal contraceptives seem to exert DNA damage and also have significant effects on blood serum enzymes. PMID:27382200

  17. Comparison of plastic vs. glass evacuated serum-separator (SST) blood-drawing tubes for common clinical chemistry determinations.

    Science.gov (United States)

    Hill, B M; Laessig, R H; Koch, D D; Hassemer, D J

    1992-08-01

    We evaluated a plastic evacuated blood-drawing tube containing an integral serum-separating barrier gel, by direct comparison with a glass counterpart. The plastic tube demonstrated no differences when compared for common clinical chemistry analytes with multiple types of instruments and systems. A total of 260 such different combinations were studied with emphasis on tests sensitive to drawing and handling indexes such as lactate dehydrogenase and potassium. A total of six separate blood drawings were tested with no significant differences noted in these tests. The total study included subjective evaluations of the plastic tube's use as a blood-drawing device and objective studies based on quantitative test results from normal and hospitalized patients and use of the primary sampling tubes (both plastic and glass) for 48-h storage. PMID:1643717

  18. In Vitro and in Vivo Wound Healing Properties of Plasma and Serum from Crocodylus siamensis Blood.

    Science.gov (United States)

    Jangpromma, Nisachon; Preecharram, Sutthidech; Srilert, Thanawan; Maijaroen, Surachai; Mahakunakorn, Pramote; Nualkaew, Natsajee; Daduang, Sakda; Klaynongsruang, Sompong

    2016-06-28

    The plasma and serum of Crocodylus siamensis have previously been reported to exhibit potent antimicrobial, antioxidant, and anti-inflammatory activities. During wound healing, these biological properties play a crucial role for supporting the formation of new tissue around the injured skin in the recovery process. Thus, this study aimed to evaluate the wound healing properties of C. siamensis plasma and serum. The collected data demonstrate that crocodile plasma and serum were able to activate in vitro proliferation and migration of HaCaT, a human keratinocyte cell line, which represents an essential phase in the wound healing process. With respect to investigating cell migration, a scratch wound experiment was performed which revealed the ability of plasma and serum to decrease the gap of wounds in a dose-dependent manner. Consistent with the in vitro results, remarkably enhanced wound repair was also observed in a mouse excisional skin wound model after treatment with plasma or serum. The effects of C. siamensis plasma and serum on wound healing were further elucidated by treating wound infections by Staphylococcus aureus ATCC 25923 on mice skin coupled with a histological method. The results indicate that crocodile plasma and serum promote the prevention of wound infection and boost the re-epithelialization necessary for the formation of new skin. Therefore, this work represents the first study to demonstrate the efficiency of C. siamensis plasma and serum with respect to their wound healing properties and strongly supports the utilization of C. siamensis plasma and serum as therapeutic products for injured skin treatment. PMID:26975771

  19. Insulin-like factor 3 levels in cord blood and serum from children: effects of age, postnatal hypothalamic-pituitary-gonadal axis activation, and cryptorchidism

    DEFF Research Database (Denmark)

    Bay, Katrine; Virtanen, Helena E; Hartung, Stefan; Ivell, Richard; Main, Katharina M; Skakkebaek, Niels E; Andersson, Anna-Maria; Toppari, Jorma

    2007-01-01

    The Leydig cell hormone insulin-like factor 3 (INSL3) is important for testicular descent. Currently INSL3 levels in cord blood, in serum throughout childhood, and in relation to congenital cryptorchidism are unknown....

  20. THE EFFECT OF GINGER ROOT POWDER (zingiber officinale SUPPLEMENTATION ON BROILER CHICKS PERFORMANCE, BLOOD AND SERUM CONSTITUENTS

    Directory of Open Access Journals (Sweden)

    W.B. ZOMRAWI

    2012-11-01

    Full Text Available A study using one hundred and twenty eight unsexed day old broiler chicks (Ross 308 32 birds/treatment with four replicates was conducted to evaluate the effect of ginger root powder as natural feed additives on growth performance, blood and serum constituents of broiler chickens. Four dietary treatments were formulated to meet the nutrient requirements of broiler chick containing ginger root powder at levels 0%, 0.5%, 1% and 1.5%. Result showed that significant decreased (P0.05 in feed conversion ratio among all dietary treatments. Treatments had significant decreased (P0.05 were observed in dressing percentage. There were no significant effect (P>0.05 on serum glucose, total protein and creatinine. Significant differences were observed in serum triglyceride and cholesterol levels. There were no significant differences (P>0.05 among all dietary treatments in Hb percentage, PCV percentage, TRBcs, MCV, MCH and MCHC percentage. The results showed that the inclusion of ginger root powder at levels 0.5% and1% in the diet , had lowering effect on cholesterol levels, and the chick may tolerate up to 1.5% without adverse effect on growth performance and blood parameters.

  1. Influence of relative blood serum amount in culture medium as well as its previous inactivation with heating on nonspecific radioactive iodine binding in vitro studies

    International Nuclear Information System (INIS)

    Nonspecific binding of radioactive iodine with the culture medium is directly proportional to the relative blood serum content for concentrations ranging 1-15%. Preliminary inactivation of the serum with heating at 56 degree C for 30 minutes, which is frequently used in in vitro studies, does not influence significantly radioactive iodine binding with culture medium

  2. Single-step bioassays in serum and whole blood with a smartphone, quantum dots and paper-in-PDMS chips.

    Science.gov (United States)

    Petryayeva, Eleonora; Algar, W Russ

    2015-06-21

    The development of nanoparticle-based bioassays is an active and promising area of research, where point-of-care (POC) diagnostics are one of many prospective applications. Unfortunately, the majority of nanoparticle-based assays that have been developed to date have failed to address two important considerations for POC applications: use of instrumentation amenable to POC settings, and measurement of analytes in biological sample matrices such as serum and whole blood. To address these considerations, we present design criteria and demonstrate proof-of-concept for a semiconductor quantum dot (QD)-based assay format that utilizes smartphone readout for the single-step, Förster resonance energy transfer (FRET)-based detection of hydrolase activity in serum and whole blood, using thrombin as a model analyte. Important design criteria for assay development included (i) the size and emission wavelength of the QDs, which had to balance brightness for smartphone imaging, optical transmission through blood samples, and FRET efficiency for signaling; (ii) the wavelength of a light-emitting diode (LED) excitation source, which had to balance transmission through blood and the efficiency of excitation of QDs; and (iii) the use of an array of paper-in-polydimethylsiloxane (PDMS)-on-glass sample chips to reproducibly limit the optical path length through blood to ca. 250 μm and permit multiplexing. Ultimately, CdSe/CdS/ZnS QDs with peak emission at 630 nm were conjugated with Alexa Fluor 647-labeled peptide substrates for thrombin and immobilized on paper test strips inside the sample cells. This FRET system was sensitive to thrombin activity, where the recovery of QD emission with hydrolytic loss of FRET permitted kinetic assays in buffer, serum and whole blood. Quantitative results were obtained in less than 30 min with a limit of detection 18 NIH units mL(-1) of activity in 12 μL of whole blood. Proof-of-concept for a competitive binding assay was also demonstrated with

  3. Dose related anxiolytic effects of diazepam: relation with serum electrolytes, plasma osmolality and systolic blood pressure (sbp) in rats

    International Nuclear Information System (INIS)

    Diazepam is an anxiolytic and anticonvulsant drug that also induces hypnosis. Changes in serum electrolyte balance, plasma osmolality and systolic blood pressure (SBP) are often associated with stress-induced anxiety. Administration of diazepam has been show to decrease stress-induced enhancement of hypothalamic pituitary adrenal cortical (HPA) axis. The present is designed to monitor the anxiolytic effects of different doses of diazepam (1 mg/kg, 2.5 mg/kg and 5 mg/kg) and its association with changes of serum electrolyte balance, plasma osmolality and SBP in rats. Administration of diazepam at doses of 1 mg/kg, 2.5 mg/kg and 5 mg/kg elicited anxiolytic effects monitored in light-dark transition test and increased serum concentration of electrolytes and plasma osmolality. Serum levels of magnesium as well as SBP decreased. The results are discussed in context of anxiolytic effects of diazepam to be mediated via a modulation of stress-induced increase in the activity of HPA-axis arid electrolytes balance. (author)

  4. Clinical significance of detection of serum markers of several viral infections in hospitalized patients before blood exposure

    International Nuclear Information System (INIS)

    Objective: To explore the desirability of setting a routine of test for detection of the serum markers of several viral infections hospitalized patients before anticipated blood exposure. Methods: Serum levels of five HBV markers, anti-HCV, anti-HIV (with ELISA) and ALT were determined in 214 hospitalized patients before forthcoming blood exposure as well as in 2468 controls. Results: The positive rate of each of the above-mentioned markers in the patients was: HBsAg 15.2% (397/2614), HBcAb- IgG 72.5% (1895/2614), anti-HCV 3.91% (102/2614), anti- HIV 0.08% (2/2614) and ALT level was above 40 u in 8.7% of the patients (227/2614). Each of the positive rate was significantly higher than that in the controls. Conclusion: There is a substantial portion of subjects harboring viral infections in the hospitalized patients. It is imperative to have these patients identified before blood exposure so that proper cautions can be taken and preventive measures implemented to minimize possible nosocomial as well as patients-to-staff infections. Moreover, any potential legal problems can also be appropriately dealt with. (authors)

  5. The Effect of Different Types of Musculoskeletal Injuries on Blood Concentration of Serum Amyloid A in Thoroughbred Racehorses.

    Directory of Open Access Journals (Sweden)

    Agnieszka Turło

    Full Text Available Training-induced muscle, skeletal and joint trauma may result in acute phase response reflected by the changes in the blood concentration of serum amyloid A (SAA in racehorses. It remains yet unclear if such systemic reaction could be triggered by sport injuries and what is the impact of different types of musculoskeletal trauma on SAA concentrations in racehorses. This study aimed to determine changes in the SAA blood concentration in racehorses with different types of injuries of musculoskeletal system.The study involved 28 racehorses diagnosed after the race with bone fractures (n = 7, dorsal metacarpal disease (n = 11, joint trauma (n = 4 or tendon and muscle trauma (n = 6 and 28 healthy control racehorses. Serum samples were collected twice, between 1 and 4 days of the injury or succesful completion of the race. SAA concentration was measured using the commercial ELISA kit. Differences between mean SAA concentration in respective groups were analyzed using ANOVA and Tukey post-hoc test.Mean SAA concentration within the first 4 days of the injury of muscle and tendon was significantly higher than in bone fractures, dorsal metacarpal disease, joint trauma or in the healthy horses (p<0,001. There were no significant differences between the other groups.Strain injuries of muscle and tendons can cause a moderate increase in SAA blood concentration in racehorses, reflecting the occurrence of the acute phase response. Similar reaction is not observed in the stress-related bone injuries.

  6. High dose flaxseed oil supplementation may affect fasting blood serum glucose management in human type 2 diabetics.

    Science.gov (United States)

    Barre, Douglas E; Mizier-Barre, Kazimiera A; Griscti, Odette; Hafez, Kevin

    2008-01-01

    Type 2 diabetes is characterized partially by elevated fasting blood serum glucose and insulin concentrations and the percentage of hemoglobin as HbA1c. It was hypothesized that each of blood glucose and its co-factors insulin and HbA1c and would show a more favorable profile as the result of flaxseed oil supplementation. Patients were recruited at random from a population pool responding to a recruitment advertisement in the local newspaper and 2 area physicians. Completing the trial were 10 flaxseed oil males, 8 flaxseed oil females, 8 safflower (placebo) oil males and 6 safflower oil females. Patients visited on two pre-treatment occasions each three months apart (visits 1 and 2). At visit 2 subjects were randomly assigned in double blind fashion and in equal gender numbers to take flaxseed oil or safflower oil for three further months until visit 3. Oil consumption in both groups was approximately 10 g/d. ALA intake in the intervention group was approximately 5.5 g/d. Power was 0.80 to see a difference of 1 mmol of glucose /L using 12 subjects per group with a p impact on fasting blood serum glucose, insulin or HbA1c levels. It is concluded that high doses of flaxseed oil have no effect on glycemic control in type 2 diabetics. PMID:18391475

  7. METABOLIC PARAMETERS CONCENTRATIONS IN BLOOD SERUM OF CZECH PIED BULLS DEPENDING ON SINGLE NUCLEOTIDE POLYMORPHISM OF LEPTIN GENE

    Directory of Open Access Journals (Sweden)

    Aleš Pavlík

    2014-02-01

    Full Text Available The aim of present study was to test hypothesis, that the leptin gene single nucleotide polymorphism (C/T giving missense mutation (Arg25Cys has an effect on concentration of blood serum total cholesterol, beta-hydroxybutyrate and urea in cattle. The experiment were performed in 58 Czech Pied bulls at 240 ± 9 days of age, which were divided in three experimental groups depending on different leptin genotypes (CC, n=28; CT, n=21; TT, n=9. Resulting genotypes in the exon 2 were CC (48.3%, CT (36.2%, and TT (15.5%. There were no differences in serum total cholesterol, urea, beta-hydroxybutyrate concentrations among the genotypes. Based on our results we may assume that analysed SNP of leptin gene have no effect on nutritional status and energy balance in fattened cattle.

  8. Serum thyroxin (T/sub 4) and thyroid stimulating hormone (TSH) levels in cord blood of newborns in Lahore

    International Nuclear Information System (INIS)

    Objective: The primary aim of this study was the early detection and treatment of hypothyroidism in neonates. This paper describes the determination of cord blood serum T/sub 4/ and TSH levels, their mean levels reference ranges and interrelationship. Results: Mean (Plus minus SD) T/sub 4) leveling cord blood serum was 115 plus minus 36 nmol/L (range: 15-350 nmol/L) and mean TSH level was found to be 5.6 plus minus 5.1 mlU/L (range: 0.05 - 150). The reference range for T/sub 4/ and TSH was 49-189 nmol/L and 0.4-17.6 mlU/L respectively. A trend in T/sub 4/ and TSH levels distributions towards higher values was noted Serum T/sub 4/ and TSH levels were not correlated significantly. Except at lowest and highest levels no reciprocal relationship between T/sub 4/ and TSH levels was found as is observed in adults. T/sub 4/ and TSH levels no reciprocal relationship between T/sub 4/ and TSH levels was found as is observed in adults. T/sub 4/ and TSH levels in 90% neonates were between 60-173 nmol/L and 1.0-14.2 mlU/L respectively. Conclusion: In most neonates, level of cord serum T/sub 4/ was independent of TSH level. Mean T/sub 4/ and TSH levels and their normal ranges were lower as compared to an iodine-sufficient country like USA. In 11.4% of neonates TSH levels were above 10 mlU/L, corresponding to mild degree of iodine deficiency disorders in Lahore. (author)

  9. Comparison Among Plasma, Serum, and Whole Blood Ethanol Concentrations: Impact of Storage Conditions and Collection Tubes

    OpenAIRE

    Penetar, David M.; McNeil, Jane F.; Ryan, Elizabeth T.; Scott E. Lukas

    2008-01-01

    How blood samples are processed and stored before being analyzed for alcohol levels is of concern in the forensic and toxicological fields, and is important in the standardization of research methods. This experiment explored for systematic differences in ethanol levels among several methods of processing and storing blood samples. Five adults consumed a standard alcoholic drink (0.7 g/kg) over a 15-min period, and blood samples were taken 5 times during a 3-h period following drinking onset....

  10. BLOOD SERUM TESTOSTERONE LEVEL AND ITS RELATIONSHIP WITH SCROTAL CIRCUMFERENCE AND SEMEN CHARACTERISTICS IN NILI-RAVI BUFFALO BULLS

    Directory of Open Access Journals (Sweden)

    M. SAJJAD, S. ALI, N. ULLAH1, M. ANWAR1, S. AKHTER AND S. M. H. ANDRABI1

    2007-04-01

    Full Text Available This study was aimed at determining the blood serum testosterone level and its relationship with scrotal circumference and physical characteristics of semen in Nili-Ravi buffalo bulls. Semen samples were collected weekly from three buffalo bulls of 14 years age for 12 weeks and were evaluated for physical characteristics i.e. ejaculatory volume, sperm motility, sperm concentration, pH and sperm abnormalities. Jugular blood samples were collected from each bull at weekly intervals and analyzed for serum testosterorse concentrations. Mean (+ SE blood serum testosterone level (ng/ml, scrotal circumference (cm, semen volume (ml, progressive sperm motility (%, sperm concentration (106/µl, semen pH and total sperm abnormalities (% observed were 0.69 ± 0.12, 34.6 ± 0.9, 3.59 ± 0.41, 51.53 ± 2.23, 0.99 ± 0.07, 7.01 ± 0.08 and 11.67 ± 0.90, respectively. Positive correlations between testosterone level and scrotal circumference (r=0.414 and ejaculatory volume (r=0.348 were observed. However, no correlation of testosterone level with sperm motility (r=0.145, sperm concentration (r=0.264, semen pH (r=-0.208 and total sperm abnormalities (r=-0.242 was found. Similary, ejaculatory volume did not show any correlation with sperm motility percentage (r=0.115, sperm concentration (r=0.045, semen pH (r=-0.015 and total sperm abnormalities (r=-0.135. Sperm motility percentage had positive correlation with sperm concentration (r=0.347 and negative correlation with semen pH (r=-0.670. Sperm concentration was negatively correlated with semen pH (r=-0.501. It was concluded that in 14 years old buffalo bulls the level of serum testosterone and scrotal circumference and ejaculatory volume were positively correlated. The other semen quality parameters including sperm motility, sperm concentration, semen pH and sperm abnormalities were not related with serum testosterone level.

  11. [Dynamics of lysozyme levels in the blood serum and milk of puerperae with various functional activities of the breasts].

    Science.gov (United States)

    Safronov, O V; Bakuleva, L P; Nesterova, A A; Babaian, S S

    1991-05-01

    The morbidity rates, ++features of lactation formation, and serum and breast milk lysozyme levels were studied in 91 parturients who showed various breast functional activity in the early postpartum period. The highest frequency of postpartum ++pyo-septic diseases was found in females with hyperlactation. There was earlier appearance of foremilk and milk in these females. Healthy mothers with higher breast functional activity exhibited the greatest levels of lysozyme in the milk and marked decreases in its levels in the blood within the first 3 days as compared to those observed in females who had normal or insufficient quantities of milk. PMID:1897671

  12. Assessment of concentration of lead, cadmium, chromium and selenium in blood serum of cancer and diabetic patients of Pakistan

    International Nuclear Information System (INIS)

    Heavy metals have specific role in the progression of diseases. The aim of this study was to investigate the level of toxic elements, i.e. lead, cadmium, chromium and selenium in blood serum of type 1 diabetes mellitus and cancer patients. Samples were collected from Institute of Nuclear Medicine and Oncology, Lahore (INMOL) Hospital and Jinnah Hospital, Lahore. They were analyzed by inductively coupled plasma optical emission spectrometry (ICP-OES). Concentrations of toxic metals, i.e. lead, cadmium and chromium have been found relatively high in both cancer and diabetic patients where as concentration of selenium was relatively lower than the normal subjects. (author)

  13. A genetic risk factor for low serum ferritin levels in Danish blood donors

    DEFF Research Database (Denmark)

    Sørensen, Erik; Grau, Katrine; Berg, Trine;

    2012-01-01

    BACKGROUND: Iron deficiency is a frequent side effect of blood donation. In recent years, several studies have described genetic variants associated with iron concentrations. However, the impact of these variants on iron levels is unknown in blood donors. Knowledge of genetic variants that predis...

  14. Enzyme-linked immunoassay for dengue virus IgM and IgG antibodies in serum and filter paper blood

    NARCIS (Netherlands)

    T.N.T. Tran; P.J. de Vries; L.P. Hoang; G.T. Phan; H.Q. Le; B.Q. Tran; C.M.T. Vo; N.V. Nguyen; P.A. Kager; N. Nagelkerke; J. Groen

    2006-01-01

    Background: The reproducibilty of dengue IgM and IgG ELISA was studied in serum and filter paper blood spots from Vietnamese febrile patients. Methods: 781 pairs of acute (t0) and convalescent sera, obtained after three weeks (t3) and 161 corresponding pairs of filter paper blood spots were tested w

  15. Enzyme-linked immunoassay for dengue virus IgM and IgG antibodies in serum and filter paper blood

    NARCIS (Netherlands)

    T.N.T. Tran (Thanh Nga); P.J. de Vries (Peter); L.P. Hoang (Lan Phuong); G.T. Phan (Giao); H.Q. Le (Hung); B.Q. Tran (Binh); C.M.T. Vo (Chi Mai); N.V. Nguyen (Nam); P.A. Kager (Piet); N.J.D. Nagelkerke (Nico); J.M. Groen (Jan)

    2006-01-01

    textabstractBackground: The reproducibilty of dengue IgM and IgG ELISA was studied in serum and filter paper blood spots from Vietnamese febrile patients. Methods: 781 pairs of acute (t0) and convalescent sera, obtained after three weeks (t3) and 161 corresponding pairs of filter paper blood spots w

  16. Relation of electrocardiographic left ventricular hypertrophy to blood pressure, body mass index, serum lipids and blood sugar levels in adult Nigerians.

    Science.gov (United States)

    Opadijo, O G; Omotoso, A B O; Akande, A A

    2003-12-01

    Left ventricular hypertrophy (LVH) is considered an independent risk factor even in the absence of systemic hypertension. Electrocardiographic (ECG) LVH with repolarisation changes has been found in some countries to carry more coronary risk than LVH alone. How far this observation is true among adult Nigerians is not known. We therefore decided to study adult Nigerians with ECG-LVH with or without ST-T waves changes and compare them with normal age matched controls (without ECG-LVH) in relation with established modifiable risk factors such as systemic hypertension (BP), body mass index (BMI), fasting blood sugar (FBS) and serum lipids such as total cholesterol (Tc), low density lipoprotein cholesterol (LDL-C), high density lipoprotein cholesterol (HDL-C) and triglyceride (TG). Adult Nigerians who were consecutively referred to the ECG laboratory were randomly recruited. Three hundred patients were studied. Their blood pressures (BP) as well as body mass indices were recorded after recording their resting 12 read ECG using portable Seward 9953 ECG machine. Their waist-hip ratio (WHR) was also recorded. Blood samples were taken to determine their fasting blood sugar and serum lipids. Their ECG tracings were read by the cardiologists involved in the study while the blood samples were analysed by the chemical pathologist also involved in the study. At the end of the ECG reading, the patients were divided into 3 groups according to whether there was no ECG-LVH (control group A), ECG-LVH alone (group B), and ECG-LVH with ST-T waves changes (group C). One hundred and fifty (50%) patients belonged to group A, 100 (33.3%) patients to group B and 50 (16.7%) group C. Group B patients were found to have higher modifiable risk factors in form of systemic BP. Tc, LDL-C, and WHR compared to group A. However, the group C patients had increased load of these coronary risk factors in terms of BP elevation, higher BMI, FBS, and scrum cholesterol compared to group B. In addition

  17. HMSRP Hawaiian Monk Seal Contaminants (Blubber, serum, and whole blood persistent organic pollutants) Data

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This data set contains information on persistent organic pollutant analysis of Hawaiian monk seal whole blood and blubber samples from the northwestern Hawaiian...

  18. [Role of Mg2+-dependent alkaline endodnaase of blood serum in tumorigenesis in A/smail mice].

    Science.gov (United States)

    Kovalenko, G A; Lel'kin, M K; Panin, L E

    2006-01-01

    An enzymoassay of levels of Mg2+-dependent alkaline endoDNAase of protein spectrum of blood serum of noninbred albino mice by SDS-electrophoresis in 10% PAAG established a fairly high heterogeneity of the enzyme. The variety of alkaline endoDNAases must be due to the limited proteolysis of their high-molecular precursor by specific proteases as described in the literature. No alkaline endoDNAase activity was identified by analysis of 10-150 kDa protein spectrum in A/smail line mice without ascites hepatoma. It was detected (pH 8.3) in the 25-45 kDa range on day 10 after tumor transplantation. Considering the gravity of disease (pre-lethal stage), on day 10, it was suggested that the level be accounted for by decay of diseased cells. The lack or extremely low level of endoDNAase activity in original blood serum expressed a mechanism of enhancing sensitivity to tumor cell effects. In mice bearing ascites hepatoma, the enzyme levels (pH 8.3) were much higher on day 10. DNAase activity (pH 7.5) was not induced in response to the heterogenous DNA. Our data point to possible defects in the induction of Mg2+-dependent alkaline endoDNAases (pH 8.3) and DNAses (pH 7.5) as well as their role in raising sensitivity to transplantable ascites hepatoma. PMID:17191709

  19. Oligosaccharide and Creatine Supplementation on Glucose and Urea Nitrogen in Blood and Serum Creatine Kinase in Basketball Athletes

    Institute of Scientific and Technical Information of China (English)

    2005-01-01

    The effects of oligosaccharide and creatine (Cr) supplementation on glucose, lactic acid and urea nitrogen levels in blood and activity of serum creatine kinase (CK) were explored. Twenty CUBA male athletes were divided into 4 groups: group A (supplementation of Cr alone), group B (supplementation of oligosaccharide), group C (supplementation of oligosaccharide and Cr) and group D (placebo control group). By using orthogonal L4 table (23), the experiment was performed. There were factors including oligosaccharide (carbohydrate, CHO), Cr and their correlation. Each factor had two levels: supplementation and no-supplementation. The results showed that the supplementation of CHO or Cr alone, combined supplementation of CHO and Cr could significantly reduce the glucose, urea nitrogen levels in blood and serum CK activity after competition in the athletes. Moreover, the effects of combined supplementation of CHO and Cr were more satisfactory. It was concluded that supplementation of CHO and Cr could promote the recovery of physical performance and athletic abilities after athletics in basketball athletes.

  20. Clinical significance of serum carbohydrate antigen 19-9 and its relationship with blood glucose in patients with type 2 diabetes

    Institute of Scientific and Technical Information of China (English)

    武秀玲

    2013-01-01

    Objective To study the relationship between serum carbohydrate antigen 19-9 (CA19-9) and blood glucose level in patients with type 2 diabetes (T2DM) .Methods Totally 784 T2DM patients and 197 healthy controls were enrolled in this study.Age,duration,body mass index (BMI) ,systolic blood pressure (SBP) ,diastolic blood pressure (DBP) ,levels of fasting blood glucose (FBG) ,postprandial blood glucose (PBG) ,hemoglobin A1c (HbA1c) ,total cholesterol (TC) ,triglyceride (TG) ,

  1. Biological indicators of radiation exposure - increase of blood serum amylase following irradiation of salivary glands

    International Nuclear Information System (INIS)

    In this paper, an isoenzyme test is described which enables the proteins of pancreas to be differentiated from those of the salivary glands. This isoenzyme test, in combination with an antibody lipase test which in turn is sensitive to the pancreatic enzyme triacylglycerinlipase, has been used to examine a total of the 42 patients under radiation therapy. 25 of these patients had been subjected to partial body exposure of the head region and 17 to whole body exposure. The results show that the amount of activity increase depends upon the dose to the salivary glands and that almost exclusively the isoenzymes of these organs are responsible for the radiation-induced activity increase in the serum. The isoenzyme test proved to be a good approach in cases where pathogenic variations in the serum α-amylase activity concerning primarily the pancreatic glands had to be differentiated from radiation-induced variations. (orig./MG)

  2. Comparison of some blood parameters, serum vitamin E and mineral concentrations of Arabian and English thoroughbred race horses

    Directory of Open Access Journals (Sweden)

    Bilal Tarik

    2004-01-01

    Full Text Available The aim of this study was to determine some blood parameters, serum vitamin E and mineral concentrations of Arabian and English thoroughbred racehorses fed the same diets. The diet was formulated to provide 2.31 Mcal DE/kg, and 10.96% crude protein. Total protein, lactate, calcium, phosphorus, potassium copper, cobalt and zinc were determined in serum obtained from 40 Arabian and 40 English healthy racing thoroughbred horses aged 2-3. The copper, cobalt and zinc concentrations were determined by atomic absorption, vitamin E by HPLC and the other biochemical parameters by a spectrophotometer. Mean values were 6.77 and 6.86 g/dl for total protein, 1.88 and 2.16 mg/dl for lactate 13.18 and 12.80 mg/dl for calcium, 4.35 and 4.39 mmol/l for phosphorus, 2.64 and 3.14 mmol/l for potassium, 129 and 166 μg/dl for copper, 36 and 44 μg/dl for cobalt and, 160 and 58 μg/dl for zinc in Arabian and English horses respectively, and Mean serum vitamin E levels were 2.65 and 2.81 μg/ml respectively. This study did not demonstrate a significant effect of breed on serum total protein, lactate, calcium, phosphorus, copper, cobalt and vitamin E. However, breed may have an effect on potassium and zinc concentration in Arabian and English thoroughbred racehorses (p<0.05.

  3. TRANSFORMING GROWTH FACTOR Β IN BLOOD SERUM AND BRONCHOALVEOLAR LAVAGE FLUID IN PATIENTS WITH CHRONIC OBSTRUCTIVE PULMONARY DISEASE

    Directory of Open Access Journals (Sweden)

    E. A. Surkova

    2008-01-01

    Full Text Available Abstract. Progressive obstruction and lung tissue remodeling comprise an important feature of the airways in COPD patients. The main processes involved in tissue remodeling in COPD are protease/antiprotease, oxidant/antioxidant imbalances, like as inflammatory and fibrotic events that contribute to development or progression of disease. TGFβ is a multifunctional growth factor that regulates synthesis of extracellular matrix proteins, primarily collagen and fibronectin, thus inducing fibrosis of respiratory ways. The aim of our study was to determine levels of TGFβ in serum and bronchoalveolar lavage fluid (BALF of COPD patients. All the patients with COPD had increased levels of TGFβ in serum, as compared with subjects without COPD (p < 0.01, but there was no difference in TGFβ concentration between patients at different stages of disease. Increased phagocytic activity of blood monocytes was found in 81% of COPD patients, as compared to controls. Phagocytosis of apoptotic T­cells and bacterial infection of monocytes leads to increased secretion of TGFβ and it may cause higher levels of TGF β in peripheral blood. TGFβ concentration in BALF of patients at stage III of disease was higher than in the patients at stage II (p < 0.05. The level of TGFβ in BALF directly correlates with number of alveolar macrophages (r = 0.39; р = 0.03. These data indicate that TGFβ is involved in chemotaxis of macrophages in COPD patients’ airways. We conclude that increased secretion of TGFβ by peripheral blood monocytes may be a result of their high phagocytic activity. Hence, TGFβ mediates interactions between the two main components underlying lung tissue remodeling, i.e. fibrosis of respiratory airways, and development of emphysema in COPD.

  4. The correlation of the serum leptin, insulin resistance, endocrine hormone, blood lipid and of simple obesity children and growth

    Institute of Scientific and Technical Information of China (English)

    Xiao-Yu Wang; Xiao-Yun Liu; Kai-Yu Xu

    2015-01-01

    Objective:To analyze the correlation of the serum leptin, insulin resistance, Endocrine hormone, blood lipid and of simple obesity children and growth.Methods:200 cases of simple obesity children (Study Group) and 200 cases of non obese children (control group) were studied, Fasting blood glucose(FBG), Glycosylated hemoglobin (HbAlc), the serum leptin detection (Leptin), insulin (INS), insulin resistance index (HOMA2-IR), growth hormone (GH), cortisol (cortisol), thyroid hormones and blood lipid indexes were detected.Results:Leptin, cortisol and T3 levels of study group were (21.39±2.29) g/L, (18.43±1.72) g/mL and (20.71±1.82) pmol/L, were significantly increased compared with the control group, and GH level was (3.15±0.41) g/L, was significantly reduced, the difference of all these indexes were statistically significant in two groups (P0.05), HbAlc, INS and HOMA2 - IR were respectively (5.82±0.87)%, (33.02-2.34) mIU/L, (3.31-0.54), compared with the control group obviously increase than the corresponding numerical of the control group, the difference of all these indexes have statistical significance in two groups (P<0.05). TC, TG and LDL-C of study group were (4.67±0.65) mmol/L, (1.62±0.45) mmol/L and (3.59±0.46) mmol/L, were significantly higher than those in the control group, while HLD-C was (1.10±0.24) mmol/L, was significantly lower (P<0.05). Conclusion:Children with simple obesity have leptin resistance, reduction of growth hormone and endocrine and lipid metabolism disorder, and seriously affect the normal growth and development of children.

  5. Blood serum concentrations of perfluorinated compounds in men from Greenlandic Inuit and European populations

    DEFF Research Database (Denmark)

    Lindh, Christian H; Rylander, Lars; Toft, Gunnar; Axmon, Anna; Rignell-Hydbom, Anna; Giwercman, Aleksander; Pedersen, Henning Sloth; Góalczyk, Katarzyna; Ludwicki, Jan K; Zvyezday, Valentyna; Vermeulen, Roel; Lenters, Virissa; Heederik, Dick; Bonde, Jens Peter; Jönsson, Bo A G

    2012-01-01

    Perfluorinated compounds (PFCs), such as perfluorooctane sulfonate (PFOS) and perfluorooctanoic acid (PFOA), are used in large quantities. They are persistent and found in measurable levels in human serum around the world. They have been associated with developmental, hepatic, and carcinogenic...... of seven PFCs were analyzed by liquid chromatography/tandem mass spectrometry (LC/MS/MS). The concentrations of PFOS and PFOA were the highest of all PFCs in all three populations with a total amount of almost 90% of the PFCs. The mean levels of PFOS and PFOA were in the Greenlandic Inuits 52 and 4...

  6. Correlation of hepatitis C RNA and serum alanine aminotransferase in hepatitis B and C seronegative healthy blood donors

    Directory of Open Access Journals (Sweden)

    Ali Natasha

    2010-07-01

    Full Text Available Introduction: Historically, serum alanine transaminase (ALT has been used as a surrogate marker in the detection of hepatitis viruses in blood donors. With the availability of newer sensitive technologies for the detection of seroconversion, the value of ALT becomes questionable but continues to be used for this purpose with subsequent discarding of ALT elevated blood units. Objective: The present study aims to evaluate the significance and cost effectiveness of ALT as a surrogate marker for hepatitis C virus infection in healthy asymptomatic blood donors who were serologically negative. Materials and Methods: The study was conducted at clinical laboratory of a tertiary care hospital for a period of one year from November 2006 to October 2007. All donors were screened serologically for hepatitis B, C and HIV I and II, syphilis and malaria and those tested positive were excluded from further evaluation. Gender-wise reference ranges and minimal and markedly raised results for ALT (described respectively as one and two folds increase above reference range were defined and, accordingly, donors were grouped into three. Two hundred seronegative blood donors were randomly selected from all three groups of ALT results and tested for hepatitis C nucleic acid through Amplicor; HCV RNA test. The cost of discarding an ALT -only elevated blood unit was also assessed. During the study period, 25117 subjects donated blood. Eight hundred and Results: seventy two donors (3.4% were positive for one or more serological tests. ALT of all donors ranged from 0-1501 U/L (Mean ± SD; 33.4 ± 25.45U/L. The donors seronegative for all disease markers were 24245 (96.6%. Of these, 21164 (87.2% donors had their ALT within reference range while 2874 (11.8% and 207 (0.8% of donors had minimal and markedly elevated results. Thus, 621 blood bags (red cells, platelets and plasma costing $ 39200.0 were discarded based on ALT results alone. Of 200 seronegative donors evaluated

  7. Growth of erythroid burst-forming units (BFU-E) in cultures of canine bone marrow and peripheral blood cells: effect of serum from irradiated dogs

    International Nuclear Information System (INIS)

    Erythroid burst-forming units (BFU-E) from canine bone marrow and peripheral blood could be grown in methylcellulose in the presence of an appropriate batch of fetal calf serum (FCS), transferrin, and erythropoietin (Epo). However, improved colony formation (size and number of bursts) was obtained when serum from total body irradiated dogs was present in the culture. This serum, obtained from dogs at day 9 after total body irradiation with a dose of 3.9 Gy, reduced markedly the Epo requirement of BFU-E. Furthermore, it allowed the omission of FCS from the culture medium if cholesterol and bovine serum albumin (BSA) were used as FCS substitutes. BFU-E concentrations were found to be rather different in the peripheral blood and in bone marrow samples from different sites (i.e., iliac crest, sternum, and humerus) of normal beagles. The studies further show that canine bone marrow BFU-E can be cryopreserved in liquid nitrogen

  8. Dramatic effect on Selenium concentration in blood serum due to the difference between the Hungarian and Indian dietary habits

    International Nuclear Information System (INIS)

    Complete text of publication follows. The importance of Selenium as trace element in animals, as well as in humans is well known. The deficiency of Selenium was observed in case of several heavy or fatal clinical events such as high infant mortality, premature, malformations and even prostate cancer. Hungary is in the top of their worldwide statistic with parallel of the low concentration of Selenium in soil as well as in food. The direct correlation can explain in Venezuela, where the prostate cancer is 'unknown disease'. The concentration of Selenium in India is higher than the worldwide level. Under the scientific bilateral cooperation 'Speciation dependent studies on physicochemical behavior of some elements in trace scale in natural and synthetic system' the Selenium was determined in human blood serum from the group of Hungarian and group of Indian patients. The samples were given in same time from each group. The main goal of the study was explain the effect of the traditional dietary habit for the level of Selenium. Therefore the samples were taken from the visiting scientist just arrived to the host institute as well as from the host scientists too. After 2 weeks stay the sampling was repeated, when the guest scientists eat the same food as the host scientists. The measurements were done in Thermo-2 ICP-MS from blood-serum separated by centrifuge. The results are summarized in Table 1. Dramatic effect was found for the level of Selenium in blood serum. As it was expected the Hungarian samples showed low concentration of Selenium in starting of the visit, as well as the high concentration was found in Indian sample, comparing to the Hungarian sample. After two weeks the Hungarian sample showed much higher concentration, however it was still less, than in Indian sample. Similar effect was found during the visit the Indian scientists in Hungary, however their Selenium concentration decreased not so spectacular due to the Selenium 'store' in body. The general

  9. Peculiarities of antithyroid autoimmunity indicators in type 2 diabetic patients depending on leptin level in blood serum and their dynamics as a result of sodium selenite treatment

    OpenAIRE

    ABRAMOVA N.O.; PASHKOVSKA N.V.; BEREZOVA M.S.

    2015-01-01

    There were studied 46 patients with diabetes mellitus type 2 in order to identify the autoimmune processes directed against thyroid tissue and dependence of those changes on the level of leptin in blood serum. It was established that in patients with high leptin serum level antithyroid antibody titer increased. In order to adjust the levels of antithyroid antibodies sodium selenite was prescribed against the background of standard therapy. Statistically significant reduction in antibodies exp...

  10. Blood Transfusion, Serum Ferritin, and Iron in Hemodialysis Patients in Africa

    OpenAIRE

    Leonard Kouegnigan Rerambiah; Laurence Essola Rerambiah; Armel Mbourou Etomba; Rose Marlène Mouguiama; Phanie Brunelle Issanga; Axel Sydney Biyoghe; Batchelili Batchilili; Sylvestre Akone Assembe; Joel Fleury Djoba Siawaya

    2015-01-01

    Background and Objectives. There is no data analyzing the outcome of blood transfusions and oral iron therapy in patients with kidneys failure in sub-Saharan Africa. The present study aimed to fill that gap and assess the value of ferritin in the diagnosis of iron overload and deficiency. Design. From January to February 2012, we prospectively studied 85 hemodialysis patients (78% of males and 22% of females aged 20 to 79 years) attending the Gabonese National Hemodialysis Centre. Results. Co...

  11. Haematological and serum biochemical indices of growing rabbits fed camel blood-rumen content mixture

    Directory of Open Access Journals (Sweden)

    Mohammed Gambo,

    2011-01-01

    Full Text Available Forty-five crossbred rabbits (Dutch × New Zealand White of mixed sexes with age between 5 and 7 weeks were divided into 5 groups of 9 rabbits and fed camel blood–rumen content mixture (CBRCM for 10 weeks. The CBRCM which contained 36.40% crude protein and 22.36% crude fibre was included at 0, 10, 20, 30 and 40% levels in diets of group 1, 2, 3, 4 and 5 respectively. The packed cell volume (PCV, red blood cells (RBC, mean corpuscular volume (MCV, mean corpuscular haemoglobin (MCH and mean corpuscular haemoglobin concentration (MCHC were not significantly different (P>0.05 among the treatments groups, although the haemoglobin (Hb and white blood cells (WBC were significantly (P<0.05 influenced by the treatments. The number of basophils, neutrophils, eosinophils and lymphocytes were not affected by the levels of inclusion, however, only the monocytes differed significantly (P<0.05 among the treatments. All values for blood parameters were within the normal range for growing rabbit. Thus, the study indicated that up to 40% CBRCM could be incorporated into the diets of growing rabbits without compromising the health status of the rabbits.

  12. Effect of parenterally administered cystamine and gammaphos (WR-2721) on some biochemical parameters in dog blood serum

    International Nuclear Information System (INIS)

    The effects were studied of intravenous and intramuscular administration of radioprotectives cystamine and gammaphos in dogs on the biochemical parameters of the blood serum. The activities were studied of enzymes aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase, creatinine kinase, gamma-glutamyl transpeptidase, lactate dehydrogenase, malate dehydrogenase, sorbitol dehydrogenase and alpha-amylase. The contents were determined of total protein, albumin, bilirubin, urea nitrogen, creatinine, glucose, triglycerides, cholesterol, lipids, calcium, sodium and potassium. Cystamine was shown to be hepatotoxic. The intramuscular administration of gammaphos was found to be more advantageous than of cystamine. Only slight increase was observed in the activities of lactate dehydrogenase, malate dehydrogenase, and alpha-amylase. With cystamine, the changes in all biochemical parameters were most marked. (M.D.). 17 figs., 18 refs

  13. Study on serum TNF-α level, B-cell count and T-cell subsets distribution in peripheral blood in patients with rheumatoid arthritis

    International Nuclear Information System (INIS)

    Objective: To study the changes of serum TNF-α levels, B-cell count and T-cell subsets distribution in peripheral blood in patients with rheumatoid arthritis. Methods: Serum TNF-α levels (with RIA), B cell as well as T cell subsets distribution type (with monoclonal antibody technique) were examined in 37 patients with rheumatoid arthritis and 30 controls. Results Serum TNF-α levels and B lymphocytes count were significantly higher in the patients than those in controls (P3, CD4 and CD4/CD8 were obviously lower (P<0.01). Conclusion: Rheumatoid arthritis is an autoimmune disease with abnormal immunoregulation. (authors)

  14. Study on the effect of food irradiation on some blood serum enzymes in rats

    International Nuclear Information System (INIS)

    Rats were fed irradiated diets as part of a study of screening tests for the safety of irradiated food. The diet consisted (g/100 g) of casein, 8.5; skim-milk, 9.4; potato starch, 50.00; wheat flour, 16.50; sucrose, 5.00; sunflower oil, 6.00; choline chloride, 0.10; salt mixture, 3.50; and vitamin mix.ure, 1.00. Diets were irradiated at 2.5 or 4.5 Mrad and were fed ad lib. for 4 months. Levels of serum (i) glutanic-pyruvic transaminase, (ii) glutamic-oxalacetic transaminase, and (iii) lactic dehydrogenase were determined. No significant changes were observed in (i) or (iii) on ieeding irradiated diets, or in (ii) for male rats. Significant decreases (P < 0.02) in (ii) were observed in female rats fed diets irradiated at 2.5 or 4.5 Mrad. (orig.)

  15. Determination of Cl, K, and Na in the blood serum of healthy elderly in the city of Sao Paulo, SP

    International Nuclear Information System (INIS)

    This work determined the blood serum concentration of Cl, K, and Na from a healthy elderly population of the city of Sao Paulo, Sao Paulo, Brazil viewing the comparison with reference values used in clinical laboratories. For collecting these samples the present work were approved by the Ethic Committee of the FMUSP and IPEN-CNEN/SP. The quality of the analytical results were evaluated by the analysis of the reference material certification NIST 1577b Bovine Liver. The results presented good accuracy and precision due to the fact that they presented relative errors less than 5.2 % and standard deviation less than 8.6 %. The average concentrations (mmol L-1) of Cl in the analysed serum were 99.1; the K were 3.9 and the Na were 136.9. The obtained data allows the to conclude that the mostly of the elderly presented Cl, K and Na concentrations in the range of reference values used in the clinical laboratories

  16. Immune complexes in blood serum of calves with clinical symptoms of bronchopneumonia

    Directory of Open Access Journals (Sweden)

    Fratrić Natalija

    2011-01-01

    Full Text Available Pneumonia in preruminant calves is a multifactorial disease. Infectious agents, the environment, management and the immune status of the calves are all important factors in determining the outcome of an infection. Until today, the level and composition of circulating immune complexes in preruminant calves with pneumonia have not been studied in detail. We performed this work with the aim to determine whether pneumonia in three-month-old calves is followed by changes in the immune complex level and changes in the γ-globulin level as their possible constituents. Immune complexes from the calves’ sera were isolated by polyethylene glycol (PEG precipitation methods. Optical density at 350 nm (OD350 of redissolved precipitates was measured to determine the circulating immune complexes level. The OD350 level of PEG precipitates of calves with pneumonia at the time of diagnosis was 0.577±0.206 and it was statistically significantly higher (p<0.001 than OD350 the level of PEG precipitates of healthy calves (0.286±0.080. Electrophoretic analysis of sera and PEG precipitates showed that both slow and fast γ-globulins are found among serum and immune-complexes' γ-globulins, but the concentration of fast γ-globulins was significantly lower in sera of diseased calves. The level of PEG precipitable immune complexes was not correlated with the concentration of serum and PEG precipitable g-globulins. The results of this study have shown that by relatively simple PEG precipitation assay it is possible to detect an increased level of circulating immune complexes in calves with pneumonia. This can be used as an additional diagnostic parameter for the detection and follow up of the disease.

  17. EFFECT OF ACUPUNCTURE OF FENGCHI (GB 20) ON BLOOD PRESSURE AND SERUM IL-6 and PLASMA ET LEVELS IN PATIENTS WITH HYPERTENSION

    Institute of Scientific and Technical Information of China (English)

    2006-01-01

    Objective: To observe the effect of acupuncture of Fengchi (风池 GB 20) on blood pressure,serum IL-6 and plasma endothelin (ET) contents in patients with hypertension. Methods: Thirty cases of hypertension patients were made up of treatment group and treated with acupuncture of Fengchi (GB 20); while 20 healthy subjects were made up of control group. Blood pressure was determined before and after the treatment. Fasting blood samples were taken from the cubital venous vessel for detecting serum interleukin (IL)-6 and plasma ET contents with radioimmunoassay before and after acupuncture treatment. Results: Before the treatment, in hypertension patients, serum IL-6 and plasma ET levels were significant higher than those in healthy subjects (P < 0.01 ); while after the treatment, blood pressure of treatment group declined significantly ( P < 0.01 ), serum IL-6 and plasma ET levels decreased considerably ( P < 0.01 ). Conclusion:Acupuncture of Fengchi (GB 20) can lower blood pressure, regulate cellular immunity and secretion functions of the vascular endothelial cells in hypertension patients.

  18. Effect of Liangxue Huoxue Xiaoyin Tang on Serum Levels of TNF-α,IFN-γ and IL-6 in Psoriasis of Blood-Heat Type

    Institute of Scientific and Technical Information of China (English)

    2005-01-01

    To explore the effect of Liangxue Huoxue Xiaoyin Tang (LHXT 凉血活血消银汤 Decoction of Removing Heat from the Blood and Promoting Blood Flow to Eliminate Psoriasis) on serum levels of tumor necrosis factor-α (TNF-α), interferon (IFN-γ) and interleukin-6 (IL-6) in psoriasis of blood-heat type. Blood samples from both the treatment group (N=33) and control group (N=30) were taken before and after treatment, and the serum levels of TNF-α, IFN-γ and IL-6 were determined by radio-immunoassay and ELISA. The total effective rate achieved in the treatment group was 90.91%. The remarkably high serum levels of TNF-α, IFN-γ and IL-6 in patients before treatment (P<0.01) were obviously decreased after one course of treatment (P<0.05) and were close to those of healthy subjects after two course of treatment (P>0.05). The data demonstrate that LHXT has the actions of reducing serum levels of TNF-α, IFN-γ and IL-6 in psoriasis of blood-heat type, and may exert a pharmacological effect targeting at the cytokines.

  19. Ontogeny and characterization of blood leukocyte subsets and serum proteins in piglets before and after weaning

    DEFF Research Database (Denmark)

    Juul-Madsen, H.R.; Jensen, K.H.; Nielsen, Jens;

    2010-01-01

    Existing knowledge about the development of the porcine immune system was extended by phenotypic characterization of leukocyte subsets and with assessment of Mannan-Binding Lectin (MBL) and immunoglobulin concentrations in peripheral blood of healthy piglets. Single-color and/or double-color flow...... immune system seem to be stimulated immediately after weaning. At the time considered to have the highest infection pressure T-cells and TLR4+ cells were markedly enhanced, whereas the expression of SLA I did not seem to be affected by weaning....

  20. Five-year weight changes associate with blood pressure alterations independent of changes in serum insulin

    DEFF Research Database (Denmark)

    Seven, Ekim; Husemoen, Lise L N; Wachtell, Kristian;

    2014-01-01

    OBJECTIVE: In overweight-related hypertension, the effect of weight changes on blood pressure (BP) is believed to be mediated by insulin. To test this hypothesis, we studied 5-year changes in weight, BP, and insulin in a general population of Danish adults (n = 3443; mean age 45.7 ± 7.6 years). M...... of the insulin variables was significantly associated with SBP or DBP (P ≥ 0.08). The results for changes in DBP were similar to SBP. CONCLUSION: Five-year weight changes associate with BP alterations independent of the insulin changes....

  1. [The effect of blood serum proteins from the seal on the analgetic action of narcotic analgesics].

    Science.gov (United States)

    Aslaniants, Zh K; Melik-Eganov, G R; Evstratov, A V; Ivanov, M P; Batrakov, S G; Korobov, N V; Iasnetsov, V V

    1991-11-01

    The protein fraction isolated from blood of seal, Phoca groenlandica, has been found to produce hyperalgesic effect on rats exposed to thermic or electrocutaneous nociceptive stimulation, but fail to affect writhes provoked by intraperitoneal injection of acetic acid solution on mice. When combined with morphine, the fraction lowered completely its narcotic analgetic action in the above mentioned tests. On the contrary, these same proteins combined with promedol or fentanil enhanced and prolonged analgetic effect of the latter. Tested in vitro the protein showed neither opioid nor anti-opioid activity. Therefore it is reasonable to suppose that neurophysiological activity of the isolated fraction is due to the peptides formed on enzymatic hydrolysis of proteins in vivo rather than these proteins as such. PMID:1687360

  2. Enzyme-linked immunoassay for dengue virus IgM and IgG antibodies in serum and filter paper blood

    Directory of Open Access Journals (Sweden)

    Tran Binh Q

    2006-01-01

    Full Text Available Abstract Background The reproducibilty of dengue IgM and IgG ELISA was studied in serum and filter paper blood spots from Vietnamese febrile patients. Methods 781 pairs of acute (t0 and convalescent sera, obtained after three weeks (t3 and 161 corresponding pairs of filter paper blood spots were tested with ELISA for dengue IgG and IgM. 74 serum pairs were tested again in another laboratory with similar methods, after a mean of 252 days. Results Cases were classified as no dengue (10 %, past dengue (55% acute primary (7% or secondary (28% dengue. Significant differences between the two laboratories' results were found leading to different diagnostic classification (kappa 0.46, p Conclusion Dengue virus IgG antibodies in serum and filter papers was not affected by duration of storage, but was subject to inter-laboratory variability. Dengue virus IgM antibodies measured in serum reconstituted from blood spots on filter papers were lower than in serum, in particular in the acute phase of disease. Therefore this method limits its value for diagnostic confirmation of individual patients with dengue virus infections. However the detection of dengue virus IgG antibodies eluted from filter paper can be used for sero-prevalence cross sectional studies.

  3. Haemolytic complement activity, C3 and FactorB consumption in serum from chickens divergently selected for antibody responses to sheep red blood cells

    NARCIS (Netherlands)

    Parmentier, H.K.; Baelmans, R.; Nieuwland, M.G.B.; Dorny, P.; Demey, F.

    2002-01-01

    Antibody responses, serum complement haemolytic activity, and complement component C3 and Factor B consumption were studied in chickens divergently selected for high and low antibody responses to sheep red blood cells, and in a randombred control line. Significantly higher total and IgG antibody res

  4. Clinical significance of determination of VCAM-1 and TNF-α in serum of patients with pregnancy-induced hypertension and in cord blood of newborns

    International Nuclear Information System (INIS)

    Objective: To study the role of vascular cell adhesion molecules (VCAM-1) and tumor necrosis factor (TNF-α) on the pathogenesis of pregnancy-induced hypertension (PIH). Methods: ELISA and radioimmunoassay were used to measure the levels of VCAM-1 and TNF-α in maternal serum and cord blood of 20 cases of normal pregnant women and 60 cases of PIH women. Results: The serum levels of VCAM-1 and TNF-α in the moderate and severe PIH group were significantly higher than that in control group (P<0.05). There was no difference of the levels of VCAM-1 and TNF-α in cord blood between PIH and control group. There was significant relativity between VCAM-1 and TNF-α in serum of PIH patients (P<0.05). conclusion: VCAM-1 and TNF-α may be important factors in the pathogenesis of PIH

  5. Maternal and Cord Blood Levels of Serum Amyloid A, C-Reactive Protein, Tumor Necrosis Factor-α, Interleukin -1β, and Interleukin-8 During and After Delivery

    Directory of Open Access Journals (Sweden)

    Luciane Marzzullo Cicarelli

    2005-01-01

    after delivery and try to correlate these proteins with tumor necrosis factor-α, interleukin -1β, and interleukin-8. Acute-phase proteins and cytokines were measured by ELISA in 24 healthy pregnant women undergoing vaginal delivery or Cesarean section. Cord blood samples in addition to maternal blood were collected. SAA and CRP reached the maximum maternal serum levels 24 hours after delivery, while cytokines remained constant over time. SAA and CRP were significantly higher in maternal serum than in newborn's (P<.001 at the moment of delivery. SAA and CRP, regardless of the type of delivery, reproduce the common pattern observed in most inflammatory conditions. Proinflammatory cytokine serum levels do not mirror the increase in SAA and CRP levels.

  6. Interday variation and effect of transportation on indirect blood pressure measurements, plasma endothelin-1 and serum cortisol in Standardbred and Icelandic horses

    Directory of Open Access Journals (Sweden)

    Söder Josefin

    2012-06-01

    Full Text Available Abstract Background Systemic hypertension is a prominent feature in humans with metabolic syndrome (MS and this is partly caused by an enhanced endothelin-1 (ET-1 mediated vasoconstriction. There are indications that systemic hypertension might be a feature in equine metabolic syndrome (EMS but if ET-1 is involved in the development of hypertension in horses is not known. Increased levels of cortisol have also been found in humans with MS but there are no reports of this in horses. Before blood pressure, plasma ET-1 and serum cortisol can be evaluated in horses with EMS, it is necessary to investigate the interday variation of these parameters on clinically healthy horses. The aims of the present study were therefore to evaluate the interday variation and influence of transportation on systemic blood pressure, plasma ET-1 and serum cortisol in healthy Standardbred and Icelandic horses, and to detect potential breed differences. Methods Nine horses of each breed were included in the study. Blood pressure was measured and blood samples were collected between 6 and 9 am on two separate days. Eight of the horses (four of each breed were transported to a new stable were they stayed overnight. The next morning, the sampling procedure was repeated. Results The interday variation was higher for plasma ET-1 (37% than for indirect pressure measurements (8-21% and serum cortisol (18%. There were no differences in systemic blood pressure between the two breeds. The Icelandic horses had significantly lower serum cortisol and significantly higher plasma ET-1 concentrations compared to the Standardbred horses. Plasma ET-1 was significantly elevated after transportation, but systemic blood pressure and serum cortisol did not differ from the values obtained in the home environment. Conclusions Indirect blood pressure, plasma ET-1 and serum cortisol are of interest as markers for cardiovascular dysfunction in horses with EMS. The elevated plasma ET-1

  7. Free phenytoin assessment in patients: measured versus calculated blood serum levels.

    Science.gov (United States)

    Tobler, Andrea; Hösli, Raphael; Mühlebach, Stefan; Huber, Andreas

    2016-04-01

    Background Total serum drug levels are routinely determined for the therapeutic drug monitoring of selected, difficult-to-dose drugs. For some of these drugs, however, knowledge of the free fraction is necessary to adapt correct dosing. Phenytoin, with its non-linear pharmacokinetics, >90 % albumin binding and slow elimination rate, is such a drug requiring individualization in patients, especially if rapid intravenous loading and subsequent dose adaptation is needed. In a prior long-term investigation, we showed the excellent performance of pharmacy-assisted Bayesian forecasting support for optimal dosing in hospitalized patients treated with phenytoin. In a subgroup analysis, we evaluated the suitability of the Sheiner-Tozer algorithm to calculate the free phenytoin fraction in hypoalbuminemic patients. Objective To test the usefulness of the Sheiner-Tozer algorithm for the correct estimation of the free phenytoin concentrations in hospitalized patients. Setting A Swiss tertiary care hospital. Method Free phenytoin plasma concentration was calculated from total phenytoin concentration in hypoalbuminemic patients and compared with the measured free phenytoin. The patients were separated into a low (35 ≤ albumin ≥ 25 g/L) and a very low group (albumin <25 g/L) for comparing and statistically analyzing the calculated and the measured free phenytoin concentration. Main outcome measures Calculated and the measured free phenytoin concentration. Results The calculated (1.2 mg/L (SD = 0.7) and the measured (1.1 mg/L (SD = 0.5) free phenytoin concentration correlated. The mean difference in the low and the very low albumin group was: 0.10 mg/L (SD = 1.4) (n = 11) and 0.13 mg/L (SD = 0.24) (n = 12), respectively. Although the variability of the data could be a bias, no statistically significant difference between the groups was found: t test (p = 0.78), the Passing-Bablok regression, the Spearman's rank correlation coefficient of r = 0

  8. UPLC-ESI-QTOF/MS and multivariate data analysis for blood plasma and serum metabolomics: effect of experimental artefacts and anticoagulant

    DEFF Research Database (Denmark)

    Barri, Thaer; Dragsted, Lars Ove

    2013-01-01

    agents, e.g. heparin, EDTA and citrate. In the present study, we looked into metabolite and other differences in matched serum and plasma samples and different plasma preparations by using untargeted UPLC-ESI-QTOF/MS profiling and multivariate data analysis (PCA and OPLS-DA). Metabolite differences...... between serum and plasma samples were mainly related to small peptides reflecting presence or absence of coagulation. Only subtle metabolite differences between the different plasma preparations were noticed, which were primarily related to ion suppression or enhancement caused by citrate and EDTA...... anticoagulants. For the first time, we also report that anticoagulant counter cation (Na+ or K+) in Na-citrate and K-EDTA plasma can make some metabolites more dominant in ESI-MS. Polymeric material residues originating from blood collection tubes for serum preparation were observed only in serum samples...

  9. Levels of organochlorine pesticides in the blood serum of agricultural workers from Rio de Janeiro State, Brazil

    Directory of Open Access Journals (Sweden)

    Francisco José Roma Paumgartten

    1998-01-01

    Full Text Available Serum levels of organochlorine pesticides (OCP were measured in agricultural workers from Rio de Janeiro State, Brazil. Blood samples from 26 volunteers (24 males, 02 females, 17-60 years old were taken in October 1997. OCP residues (op'DDT pp'DDT, pp'DDD, pp'DDE, aldrin, dieldrin, endrin, heptachlor, heptachlor-epoxide, alpha-, beta- and gamma-hexachlorocyclohexane, and hexachlorobenzene were analyzed by gas chromatography with an electron capture detector. Tests detected pp'DDE in 16 out of 26 samples, but pp'DDE concentration exceeded 1.4 µg/L (i.e. 1.8, 2.4 and 4.4 µg/L in only 3 of these. beta-HCH was found in 6 (23.1% out of 26 samples. In one sample beta-HCH did not exceed 1.4 µg/L, but in the remaining samples concentrations ranged from 1.4 to 5.3 µg/L. The percentage of positive pp'DDE samples increased from the youngest (£29 yrs: 30.0% to the oldest age group (³ 40 yrs: 100%. A similar trend was found for beta-HCH contamination (£ 29 yrs: 0%; 30-39 yrs: 20.0%; ³40 yrs: 66.7%. Dieldrin (3.7 µg/L was found in only one sample. No other OCP residue was found in the samples. Serum concentrations of OCPs found in this study are comparable to levels reported for the non-occupationally exposed population in Brazil and elsewhere.

  10. FRUCTOOLIGOSACCHARIDE (FOS SUPPLEMENTATION IN TYPE 2 DIABETIC ADULTS IMPROVES SYSTOLIC BLOOD PRESSURE, SERUM LIPID, AND GUT MICROBIOTA

    Directory of Open Access Journals (Sweden)

    Mahendra A

    2013-12-01

    Full Text Available Fructooligosaccharide (FOS is a prebiotic, becoming apparent for its therapeutic role for diseased conditions like CHD, hypertension, diabetes and obesity. CHD and hypertension are one of the most prevalent NCD’s encountered in diabetic adults. Present study aims to assess the effect of FOS supplementation in type 2 diabetic adults on their lipemic, biophysical parameters and gut microflora parameters. A cross-sectional study was designed with 65 adult type 2 diabetics enrolled from Health clinic of The M.S. University of Baroda, Gujarat. All the subjects were randomly divided into two groups control and experimental. The experimental group was given 10 g of fructooligosaccharide and compared with the controls for lipemic parameters, hypertension and fecal counts in terms of lactic acid bacteria, bifidobacteria and enteric pathogen. Eight week supplementation of FOS resulted in an appreciable reduction in serum TC, TG and LDL levels by 10%, 4.9% and 7.8% respectively. A significant reduction (p<0.05 in systolic blood pressure was also observed as a result of FOS supplementation in the experimental group. A decline was seen in TC/HDL, LDL/HDL and non-HDL by 10.4%, 7.6% and 6.6% respectively (p<0.05, p<0.001. Gut microbiota values exhibited a significant increment in fecal log10 counts of Lactic acid bacteria and bifidobacteria by 9.3% and 10.9% respectively (p<0.001 while a significant reduction by 4.8% (p<0.001 was observed for Enteric pathogen. These outcomes revealed an efficacy of FOS in reducing serum lipid parameters, systolic BP and improving the gut microbiota in type 2 diabetic adults

  11. Apoptosis-related deregulation of proteolytic activities and high serum levels of circulating nucleosomes and DNA in blood correlate with breast cancer progression

    International Nuclear Information System (INIS)

    As cell-free circulating DNA exists predominantly as mono- and oligonucleosomes, the focus of the current study was to examine the interplay of circulating nucleosomes, DNA, proteases and caspases in blood of patients with benign and malignant breast diseases. The concentrations of cell-free DNA and nucleosomes as well as the protease and caspase activities were measured in serum of patients with benign breast disease (n = 20), primary breast cancer (M0, n = 31), metastatic breast cancer (M1, n = 32), and healthy individuals (n = 28) by PicoGreen, Cell Death Detection ELISA, Protease Fluorescent Detection Kit and Caspase-Glo®3/7 Assay, respectively. Patients with benign and malignant tumors had significantly higher levels of circulating nucleic acids in their blood than healthy individuals (p = 0.001, p = 0.0001), whereas these levels could not discriminate between benign and malignant lesions. Our analyses of all serum samples revealed significant correlations of circulating nucleosome with DNA concentrations (p = 0.001), nucleosome concentrations with caspase activities (p = 0.008), and caspase with protease activities (p = 0.0001). High serum levels of protease and caspase activities associated with advanced tumor stages (p = 0.009). Patients with lymph node-positive breast cancer had significantly higher nucleosome levels in their blood than node-negative patients (p = 0.004). The presence of distant metastases associated with a significant increase in serum nucleosome (p = 0.01) and DNA levels (p = 0.04), and protease activities (p = 0.008). Our findings demonstrate that high circulating nucleic acid concentrations in blood are no indicators of a malignant breast tumor. However, the observed changes in apoptosis-related deregulation of proteolytic activities along with the elevated serum levels of nucleosomes and DNA in blood are linked to breast cancer progression

  12. Diagnostic potential for gold nanoparticle-based surface-enhanced Raman spectroscopy to provide colorectal cancer screening using blood serum sample

    Science.gov (United States)

    Lin, Duo; Feng, Shangyuan; Pan, Jianji; Chen, Yanping; Lin, Juqiang; Sun, Liqing; Chen, Rong

    2012-03-01

    Surface-enhanced Raman spectroscopy (SERS) is a vibrational spectroscopic technique that is capable of probing the biomolecular changes associated with diseased transformation. The objective of our study was to explore gold nanoparticle based SERS to obtain blood serum biochemical information for non-invasive colorectal cancer detection. SERS measurements were performed on two groups of blood serum samples: one group from patients (n = 38) with pathologically confirmed colorectal cancer and the other group from healthy volunteers (control subjects, n = 45). Tentative assignments of the Raman bands in the measured SERS spectra suggested interesting cancer specific biomolecular changes, including an increase in the relative amounts of nucleic acid, a decrease in the percentage of saccharide and proteins contents in the blood serum of colorectal cancer patients as compared to that of healthy subjects. Principal component analysis (PCA) of the measured SERS spectra separated the spectral features of the two groups into two distinct clusters with little overlaps. Linear discriminate analysis (LDA) based on the PCA generated features differentiated the nasopharyngeal cancer SERS spectra from normal SERS spectra with high sensitivity (97.4%) and specificity (100%). The results from this exploratory study demonstrated that gold nanoparticle based SERS serum analysis combined with PCA-LDA has tremendous potential for the non-invasive detection of colorectal cancers.

  13. Non-enzymatic amperometric detection of hydrogen peroxide in human blood serum samples using a modified silver nanowire electrode.

    Science.gov (United States)

    Thirumalraj, Balamurugan; Zhao, Duo-Han; Chen, Shen-Ming; Palanisamy, Selvakumar

    2016-05-15

    In this paper, we report a highly sensitive amperometric H2O2 sensor based on silver nanowires (AgNWs) modified screen printed carbon electrode. The AgNWs were synthesized using polyol method. The synthesized AgNWs were characterized by scanning electron microscopy, UV-vis spectroscopy and X-ray diffraction techniques. The average diameter and length of the synthesized AgNWs were found as 86±5 and 385nm, respectively. Under optimum conditions, the AgNWs modified electrode shows a stable amperometric response for H2O2 and was linear over the concentrations ranging from 0.3 to 704.8μM. The non-enzymatic sensor showed a high sensitivity of 662.6μAmM(-1)cm(-2) with a detection limit of 29nM. The response time of the sensor was found as 2s. Furthermore, the AgNWs modified electrode exhibited a good recovery of H2O2 (94.3%) in the human blood serum samples. PMID:26939075

  14. The Comparative Evaluation of the Effect of the Foods with Different Glycemic Indices on Blood Glucose and Serum Free Fatty Acids in Cycling, Male Athletes

    Directory of Open Access Journals (Sweden)

    Asadi, J. (PhD

    2014-05-01

    Full Text Available Background and Objective: Carbohydrates are considered as the major source of energy in physical activity. Studies show that consumption of carbohydrate foods before exercise can balance blood glucose and free fatty acids and increase athletes’ performance. In this study , we compared the effect of three kinds of foods with different glycemic indices on blood glucose (BG and serum free fatty acids (FFA in cycling ,male athletes. Material and Methods: In this clinical trial, 21 members of national cycling team randomly allocated to three equal groups of glucose (low glycemic index ، lentil (low glycemic index and potato (high glycemic index. First, Fasting blood samples (5ml were obtained to measure BG and FFA . Then the subjects were asked to eat their foods. After 45 mins of rest, they pedaled with maximal oxygen consumption VO2max for two hours and again their blood samples were taken to compare with the levels of before interventions. Results: Glucose consumption resulted in a significant decrease in FFA level after 2 hours of pedaling (P = 0.01 but no significant change in BG level. Plasma glucose was higher after eating lentil than that of potato (P<0.05, but it was not true for FFA level of both groups. Conclusion: Based on the results, the pre-exercise use of low glycemic index (lentil compared to high glycemic index (potato can better lead to increased blood glucose during exercise. Keywords: Glycemic Index; Blood Glucose; Serum Free Fatty Acids; Cyclists

  15. Proliferation and apoptosis property of mesenchymal stem cells derived from peripheral blood under the culture conditions of hypoxia and serum deprivation

    Institute of Scientific and Technical Information of China (English)

    FU Wei-li; JIA Zhu-qing; WANG Wei-ping; ZHANG Ji-ying; FU Xin; DUAN Xiao-ning; LEUNG Kevin Kar Ming; ZHOU Chun-yan; YU Jia-kuo

    2011-01-01

    Background The proliferation and apoptosis property of mesenchymal stem cells derived from peripheral blood (PB-MSCs) were investigated under hypoxia and serum deprivation conditions in vitro so as to evaluate the feasibility for autologous PB-MSCs applications in cartilage repair.Methods MSCs were mobilized into peripheral blood by granulocyte colony stimulating factor (G-CSF) and AMD3100.The blood samples were collected from central ear artery of rabbits.Adhered cells were obtained by erythrocyte lysis buffer and identified as MSCs by adherence to plastic,spindle shaped morphology,specific surface markers,differentiation abilities into osteoblasts,adipocytes and chondroblasts in vitro under appropriate conditions.MSCs were cultured in four groups at different oxygen tension (20% O2 and 2% O2),with or without 10% fetal bovine serum (FBS)conditions:20% O2 and 10% FBS complete medium (normal medium,N),20% O2 and serum deprivation medium (D),2% O2 and 10% FBS complete medium (hypoxia,H),2% O2 and serum deprivation (HD).Cell proliferation was determined by CCK-8 assay.Apoptosis was detected by Annexin V/Pl and terminal deoxynucleotide transferase dUTP nick end labeling (TUNEL) staining.Results Spindle-shaped adherent cells were effectively mobilized from peripheral blood by a combined administration of G-CSF plus AMD3100.These cells showed typical fibroblast-like phenotype similar to MSCs from bone marrow (BM-MSCs),and expressed a high level of typical MSCs markers CD29 and CD44,but lacked in the expression of hematopoietic markers CD45 and major histocompatibility complex Class Ⅱ (MHC Ⅱ).They could also differentiate into osteoblasts,adipocytes and chondroblasts in vitro under appropriate conditions.No significant morphological differences were found among the four groups.It was found that hypoxia could enhance proliferation of PB-MSCs regardless of serum concentration,but serum deprivation inhibited proliferation at the later stage of culture

  16. Changes of Leptin Level in Serum, Cord Venous Blood and Placenta in Women with Gestational Diabetes Mellitus and Correlations with Insulin and C-Peptide

    Institute of Scientific and Technical Information of China (English)

    Jin-ming ZHU; Li SHI; Xiao-yuan LU; Rong-rong ZHANG; Min LI; Xiao-ning ZHANG

    2014-01-01

    ObjectiveTo investigate the changes of leptin levels in serum, cord venous blood and placenta and examine the possible relationships among them in women with gestational diabetes mellitus (GDM).Methods This case-control study was performed in 40 women with GDM and 40 normal women. The women with GDM received dietary advice, blood glucose monitoring and insulin treatment when necessary. Maternal serum and venous cord blood leptin, insulin and C-peptide levels were detected by ELISA. The expression level of leptin in placenta was measured by an immunohistochemical method. Results1. Leptin, insulin and C-peptide levels in serum of the women with GDM were significantly higher than those of the normal women (P<0.01,P<0.05, P<0.01). 2. Cord venous blood leptin and insulin in GDM group were significantly higher than in the normal group (P<0.01,P<0.05). No significant difference was found in cord venous blood C-peptide between two groups. 3. The expression of placenta leptin protein was significantly higher in GDM women than in normal women (P<0.01). There were positive correlations between placental leptin, cord venous leptin and insulin, birth weight, ponderal index in GDM women (r=0.37,P<0.05;r=0.39,P<0.05;r=0.53,P<0.01;r=0.54,P<0.01). However, there was no correlation between placental leptin and serum leptin.Conclusion The women with GDM and their fetuses suffer from hyperleptinaemia and hyperinsulinaemia. In the women with GDM, there was disorder in the interaction between leptin and insulin. The GDM women and their fetuses may be prone to insulin resistance and leptin resistance in late pregnancy.

  17. The levels of blood mercury and inflammatory-related neuropeptides in the serum are correlated in children with autism spectrum disorder.

    Science.gov (United States)

    Mostafa, Gehan Ahmed; Bjørklund, Geir; Urbina, Mauricio A; Al-Ayadhi, Laila Yousef

    2016-06-01

    Tachykinins (substance P, neurokinin A, and neurokinin B) are pro-inflammatory neuropeptides that may play an important role in some autoimmune neuroinflammatory diseases, including autism spectrum disorder (ASD). Mercury (Hg) is a neurotoxicant, and potentially one of the main environmental triggers for ASD as it induces neuroinflammation with a subsequent release of neuropeptides. This is the first study to explore the potentially causal relationship between levels of serum neurokinin A and blood mercury (BHg) in children with ASD. Levels of serum neurokinin A and BHg were measured in 84 children with ASD, aged between 3 and 10 years, and 84 healthy-matched children. There was a positive linear relationship between the Childhood Autism Rating Scale (CARS) and both serum neurokinin A and BHg. ASD children had significantly higher levels of serum neurokinin A than healthy controls (P < 0.001). Increased levels of serum neurokinin A and BHg were respectively found in 54.8 % and 42.9 % of the two groups. There was significant and positive linear relationship between levels of serum neurokinin A and BHg in children with moderate and severe ASD, but not in healthy control children. It was found that 78.3 % of the ASD patients with increased serum levels of neurokinin A had elevated BHg levels (P < 0.001). Neuroinflammation, with increased levels of neurokinin A, is seen in some children with ASD, and may be caused by elevated BHg levels. Further research is recommended to determine the pathogenic role of increased levels of serum neurokinin A and BHg in ASD. The therapeutic role of tachykinin receptor antagonists, a potential new class of anti-inflammatory medications, and Hg chelators, should also be studied in ASD. PMID:26738726

  18. Study on the relationship between the serum thrombopoietin (TPO) levels and blood platelet counts in patients with various kinds of liver diseases

    International Nuclear Information System (INIS)

    Objective: To study the relationship between serum thrombopoietin (TPO) levels and thrombocytopenia in patients with various kinds of liver diseases. Methods: Serum TPO levels were determined with ELISA in 102 patients with various kinds of liver diseases (acute hepatitis n=9, chronic hepatitis n=80, hepatic cirrhosis n=13, etiology, HBV infection n=73, HBV + HEV n=13, HEV n=8, HCV n=6, autoimmune hepatitis n=2) and 30 controls. Results: The serum TPO levels in patients with various kinds of liver diseases were not significantly different from those in the controls (P>0.05). However, the blood platelet counts were significantly lower in all the patients (with the exception of the nine patients with acute hepatitis) than those in the controls (P 0.05). Conclusion: The lower TPO levels in patients with liver diseases could be only one of the many factors leading to thrombocytopenia. (authors)

  19. Effect Of Garlic Oil On Haematological Parameters, Blood Respiratory Functions And Serum Testosterone In Male Rats Exposed To An Electromagnetic Field

    International Nuclear Information System (INIS)

    This study was conducted to investigate the biological effects of exposure to an electromagnetic field (EMF, 900 MHz) emitted from mobile base station antenna for 4 weeks on haematological parameters, respiratory function of blood (blood gases, acid-base status) and oxygen equilibrium curves beside the levels of serum testosterone and possible protective role of garlic oil (250 mg/kg b.w/day for 4 weeks) in male albino rats. The results revealed significant reductions in haemoglobin (Hb) contents, total leucocytes (WBC) count, mean corpuscular volume (MCV) and haematocrit value (Hct %) in rats exposed to EMF (GI). These reductions have been ameliorated by garlic oil treatment (GII). Significant increases in oxygen partial pressure (PO2) were observed in arterial (a) and alveolar (A) blood. These increases were reduced by garlic oil treatment. The percent oxygen saturation (% O2 sat) was significantly decreased in arterial while it was increased significantly in venous blood in both treated groups. There was a significant reduction in carbon dioxide partial pressure (PCO2) in the arterial blood after exposure to EMF (GI) which was decreased by garlic oil treatment. Significant reductions were recorded in arterial blood pH, bicarbonate (HCO-3), total carbon dioxide (TCO2) and arterial and venous blood base excess (BE). These reductions were decreased by garlic oil treatment in GII. The blood oxygen equilibrium curves (OEC) of (GI) and (GII) were shifted to the right compared to that of control group with non-significant increases in P50 . The level of serum testosterone was highly significantly decreased in GI. This reduction was decreased in garlic oil treated group (GII). It can be concluded that garlic oil has a protective role against the hazardous effects of EMF.

  20. A study on the effects of the estrous cycle on uterine fluid and blood serum immunoglobulin G (IgG content in the cow

    Directory of Open Access Journals (Sweden)

    Sayed Mortaza Alavi-Shoushtari

    2014-06-01

    Full Text Available To investigate the IgG content and its variations in uterine fluid (UF during the estrous cycle of the cow and to compare them with those of the blood serum (S, six pairs of serum and UF samples for each phase of the cycle selected out of 240 bovine genital tracts and blood samples were collected from Urmia abattoir. The UF samples were collected by gentle scraping of the endometrium using a curette after uterine incision and their IgG content and those of the serum were measured by single radial immuno-diffusion (SRID assay. Serum IgG values (Mean ± SEM were generally higher than the UF values throughout the cycle except for di-estrus (S: 38.50 ± 0.90, UF: 51.60 ± 2.10 mg mL-1, in which the highest values were observed in UF samples. In met-estrus the difference was not significant (S: 34.80 ± 1.80mg mL-1, UF: 30.80 ± 5.20 mg mL-1, however, in estrus the mean UF IgG value (12.50 ± 1.10 mg mL-1 was lower than that of the serum (31.30 ± 1.20 mg mL-1. In pro-estrus, the lowest values (S: 27.80 ± 1.30 mg mL-1, UF: 9.10 ± 1.50 mg mL-1 were obtained. The results showed a lower IgG values in the bovine UF than those of the serum in the follicular phase of the cycle, while in di-estrus the UF IgG content was the highest, suggesting some IgG production in the uterus at this phase.

  1. Influence of different periods of the year and age on the parameters of antioxidative status and oxidative stress in the blood serum of breeding bulls.

    Science.gov (United States)

    Žaja, Ivona Žura; Samardžija, Marko; Vince, Silvijo; Majić-Balić, Ivanka; Đuričić, Dražen; Milinković-Tur, Suzana

    2016-06-01

    The sources of variations that may cause physiological differences between blood serum biochemistry parameters of bulls have not been investigated in detail. Aim of the present study was to establish influence of different periods of the year and the age of breeding bulls on parameters of antioxidative status and oxidative stress in their serum and to correlate these monitored variables. Research was performed on two groups, each comprising 9 Simmental bulls: a younger group (YB) (aged 2-4 years) and older one (OB) (aged 5-10 years). Blood samples for biochemical analyses were collected from jugular vein in cold (CP) and warm periods (WP) of the year. Reduced glutathione (GSH), uric acid (UA), total protein (TP), albumin (ALB), thiobarbituric acid reactive substance (TBARS), and protein carbonyl content (PCC) serum concentration were determined, as well as activities of selenium-dependent glutathione peroxidase (Se-GSH-Px), total superoxide dismutase (TSOD), manganese superoxide dismutase (MnSOD), copper-zinc superoxide dismutase (CuZnSOD) and catalase (CAT). Serum values of SeGSH-Px, MnSOD, UA and TP in OB were significantly higher compared to those in YB during CP of the year. Significantly higher PCC concentration in serum of YB and OB were established in CP of the year than in WP. TBARS serum concentration in YB was significantly higher in comparison to that in OB during CP of the year. It can be concluded that both OB and YB show a great sensitivity to climate condition alterations during CP in comparison to WP of the year and that YB show even greater sensitivity. PMID:27288340

  2. Phthalate Diesters and Their Metabolites in Human Breast Milk, Blood or Serum, and Urine as Biomarkers of Exposure in Vulnerable Populations

    OpenAIRE

    Högberg, Johan; Hanberg, Annika; Berglund, Marika; Skerfving, Staffan; Remberger, Mikael; Calafat, Antonia M.; Filipsson, Agneta Falk; Jansson, Bo; Johansson, Niklas; Appelgren, Malin; Håkansson, Helen

    2007-01-01

    Background Phthalates may pose a risk for perinatal developmental effects. An important question relates to the choice of suitable biological matrices for assessing exposure during this period. Objectives This study was designed to measure the concentrations of phthalate diesters or their metabolites in breast milk, blood or serum, and urine and to evaluate their suitability for assessing perinatal exposure to phthalates. Methods In 2001, 2–3 weeks after delivery, 42 Swedish primipara provide...

  3. Detection of Canine Distemper Virus Nucleoprotein RNA by Reverse Transcription-PCR Using Serum, Whole Blood, and Cerebrospinal Fluid from Dogs with Distemper

    OpenAIRE

    Frisk, A. L.; König, M.; Moritz, A; Baumgärtner, W.

    1999-01-01

    Reverse transcription-PCR (RT-PCR) was used to detect canine distemper virus (CDV) nucleoprotein (NP) RNA in serum, whole blood, and cerebrospinal fluid (CSF) samples from 38 dogs with clinically suspected distemper. Results were correlated to clinical findings, anti-CDV neutralizing antibody titers, postmortem findings, and demonstration of CDV NP antigen by immunohistochemistry. The specificity of the RT-PCR was ensured by amplification of RNA from various laboratory CDV strains, restrictio...

  4. Phenobarbital and Phototherapy Combination Enhances Decline of Total Serum Bilirubin and May Decrease the Need for Blood Exchange Transfusion in Newborns with Isoimmune Hemolytic Disease

    OpenAIRE

    Mahmoud A.F. Kaabneh; Ghassan S. A. Salama; Ayoub G.A Shakkoury; Ibrahim M. H. Al-abdallah; Afrah Alshamari; Ruba A.A. Halaseh

    2015-01-01

    OBJECTIVE The objective of this study was to evaluate the effect of phenobarbital and phototherapy combination on the total serum bilirubin of the newborn infants with isoimmune hemolytic disease (IHD) and its impact on blood exchange transfusion rates. PATIENTS AND METHOD This single-blinded, prospective, randomized, controlled trial was conducted between March 2013 and December 2014 at the pediatric ward of two Military Hospitals in Jordan. A total of 200 full-term neonates with IHD were di...

  5. Chemiluminescent analysis different methods in evaluation of lipoproteins free radical superoxide oxidation of human blood serum during different pathological processes development in organism

    International Nuclear Information System (INIS)

    Different forms of spontaneous, photo- and electro-chemiluminescence intensity was determined for human blood serum. The marked difference in intensity was revealed between normal and pathological processes development states in organism. It is suggested that the difference could be caused by lipoproteins free radical oxidation processes development with formation of radicals which are responsible for emission of light quants. Lipoproteins superoxide oxidation level change was confirmed by determination of malone dialdehide, dien conjugates as well as tocopherol and activity of superoxide dismutase

  6. Increased apoptosis of peripheral blood mononuclear cells in patients with perennial allergic asthma/rhinitis: relation to serum markers of apoptosis

    Directory of Open Access Journals (Sweden)

    Janina Grzegorczyk

    2002-01-01

    Full Text Available Background: The goal of our study was to examine spontaneous and stimulated apoptosis of peripheral blood MNC from allergic patients, sensitized to Der p I antigen as compared to cells from non-atopic subjects. Furthermore we aimed to investigate which populations of mononuclear cells (lymphocytes, monocytes undergo the apoptosis and to determine relations between apoptosis and serum levels of sFas/APO-1, ICE/caspase-1 or TNF-α.

  7. Some impressions from using new IZINTA test-kits for determining blood serum levels of T3, T4 and TTX

    International Nuclear Information System (INIS)

    The specimens of new test-kits produced by Izinta (HU) are characterized with good precision, sensitivity and specificity. The authors performed comparative determinations and the results received are satisfactory (p<0.05). The blood serum level of T3, T4 and TTX in patients with different pathology is determined. The results are confirmed by the clinical diagnosis. 2 tabs., 4 refs. (orig.)

  8. Alkaline phosphatase and transaminase activity in rat liver and blood serum at delayed periods following external γ-irradiation combined with internal exposure to plutonium 239

    International Nuclear Information System (INIS)

    A study was made of activity of alkaline phosphatase and alanine- and aspartate aminotransferase in rat liver and blood serum at remote times after external γ-irradiation combined with internal exposure to 239Pu nitrate delivered in two chronically effective doses. The radionuclide was shown to be mainly responsible for the changes observed in activity of the enzymes under study. The degree to which the changes were manifest depended upon dose of plutonium administered

  9. Analysis of Genotype 1b Hepatitis C Virus IRES in Serum and Peripheral Blood Mononuclear Cells in Patients Treated with Interferon and Ribavirin

    OpenAIRE

    Iwona Bukowska-Ośko; Kamila Caraballo Cortés; Agnieszka Pawełczyk; Rafał Płoski; Maria Fic; Karol Perlejewski; Urszula Demkow; Hanna Berak; Andrzej Horban; Tomasz Laskus; Marek Radkowski

    2014-01-01

    Hepatitis C virus (HCV) highly conserved IRES (internal ribosome entry site) sequence, localized within the 5′-untranslated region (5′UTR), may determine viral properties like replication efficiency and cell tropism. The aim of the present study was to characterize newly emerging 5′UTR variants in serum and peripheral blood mononuclear cells (PBMC) in chronic hepatitis C patients treated with interferon (IFN) and ribavirin and to identify their effect on IRES secondary structures. The study g...

  10. Fluoride Levels in Urine, Blood Plasma and Serum of People Living in an Endemic Fluorosis Area in the Thar Desert, Pakistan

    International Nuclear Information System (INIS)

    Fluoride (F/sup -/) levels in the urine, blood plasma and serum samples of people living in a village in the Thar Desert have been analysed where fluorosis is endemic and groundwater contains fluoride as high as 9.76 mg/L. Average values of fluoride levels in these human specimens i.e. urine, blood plasma and serum have been found to be 12.98 mg/L, 0.61 mg/L and 0.75 mg/L, respectively. These values are much higher than the normal values reported from other parts of the world. It was observed that urinary fluoride levels increase with age of an individual but with an insignificant correlation (r = 0.116), however, fluoride levels in plasma and serum samples did not vary considerably with age in male and female subjects. It has been found that after the age of 50 years the urinary fluoride decreases with its concomitant increase in serum fluoride level. Since groundwater is the only available source of water for human consumption and it contains higher fluoride (av. 7.09 mg/L) than WHO limit (1.5 mg/L), therefore, it is the main cause of fluorosis in the area. (author)

  11. Ketones blood test

    Science.gov (United States)

    ... Ketones - serum; Nitroprusside test; Ketone bodies - serum; Ketones - blood ... A blood sample is needed. ... When the needle is inserted to draw blood, some people feel slight ... there may be some throbbing or a slight bruise. This soon ...

  12. Positive Reinforcement Training for Blood Collection in Grizzly Bears (Ursus arctos horribilis) Results in Undetectable Elevations in Serum Cortisol Levels: A Preliminary Investigation.

    Science.gov (United States)

    Joyce-Zuniga, Nicole M; Newberry, Ruth C; Robbins, Charles T; Ware, Jasmine V; Jansen, Heiko T; Nelson, O Lynne

    2016-01-01

    Training nonhuman animals in captivity for participation in routine husbandry procedures is believed to produce a lower stress environment compared with undergoing a general anesthetic event for the same procedure. This hypothesis rests largely on anecdotal evidence that the captive subjects appear more relaxed with the trained event. Blood markers of physiological stress responses were evaluated in 4 captive grizzly bears (Ursus arctos horribilis) who were clicker-trained for blood collection versus 4 bears who were chemically immobilized for blood collection. Serum cortisol and immunoglobulin A (IgA) and plasma β-endorphin were measured as indicators of responses to stress. Plasma β-endorphin was not different between the groups. Serum IgA was undetectable in all bears. Serum cortisol was undetectable in all trained bears, whereas chemically immobilized bears had marked cortisol elevations (p < .05). The highest cortisol elevations were found in 2 bears with extensive recent immobilization experience. These findings support the use of positive reinforcement training for routine health procedures to minimize anxiety. PMID:26847149

  13. Detection of Babesia bovis in blood samples and its effect on the hematological and serum biochemical profile in large ruminants from Southern Punjab

    Institute of Scientific and Technical Information of China (English)

    Samreen Zulfiqar; Ali Saeed; Furhan Iqbal; Sadia Shahnawaz; Muhammad Ali; Arif Mahmood Bhutta; Shahid Iqbal; Sikandar Hayat; Shazia Qadir; Muhammad Latif; Nazia Kiran

    2012-01-01

    Objective:To determine the presence of Babesia bovis (B. bovis) in large ruminants in southern Punjab and its effect on hematological and serum biochemical profile of host animals. Methods:Blood samples were collected from 144 large ruminants, including 105 cattle and 39 buffaloes, from six districts in southern Punjab including Multan, Layyah, Muzaffar Garh, Bhakar, Bahawalnagar and Vehari. Data on the characteristics of animals and herds were collected through questionnaires. Different blood (hemoglobin, glucose) and serum (ALT, AST, LDH, cholesterol) parameters of calves and cattle were measured and compared between parasite positive and negative samples to demonstrate the effect of B. bovis on the blood and serological profile of infected animals. Results:27 out of 144 animals, from 5 out of 6 sampling districts, produced the 541-bp fragment specific for B. bovis. Age of animals (P=0.02), presence of ticks on animals (P=0.04) and presence of ticks on dogs associated with herds (P=0.5) were among the major risk factors involved in the spread of bovine babesiosis in the study area. ALT concentrations were the only serum biochemical values that significantly varied between parasite positive and negative cattle. Conclusions:This study has reported for the first time the presence of B. bovis in large ruminant and the results can lead to the prevention of babesiosis in the region to increase the livestock output.

  14. Investigations on the presence of antibodies against equine herpesvirus-1 in blood serum of foals, prior to and after colostrum intake

    Directory of Open Access Journals (Sweden)

    Lauš Saša

    2015-01-01

    Full Text Available The titer of specific antibodies against equine herpesvirus-1 in blood serum was tested in two groups of mares and their foals. The first group consisted of 12 mares, Standardbred and Serbian Trotter breed, who were vaccinated against equine herpesvirus-1 and 4 in the 5th, 7th and 9th month of pregnancy. On the contrary, 12 mares from the second group, of Lipizzaner breed, were not vaccinated. The mares’ blood samples for antibodies titer investigation were taken 30, 15 and 7 days before the expected partus, then immediately after the partus, while their foals’ blood samples were taken immediately after foaling, then just before colostrum intake, and finally 1, 2, 3 and 7 days later. The titer of antibodies against equine herpesvirus-1 was tested by the method of virus - neutralization, on microtiter plates with constant dose of the virus and serial double dilutions of the serum. In unvaccinated mares, titer of antibodies against equine herpesvirus-1 was either low or not present, but on the contrary, in the vaccinated ones the antibodies titer ranged from 1:32 to 1:256. In the foals originating from both vaccinated and unvaccinated there were not found specific antibodies in the serum before colostrum intake. After the colostrum intake, the values of specific antibodies against equine herpesvirus-1 significantly increased in the foals originating from the vaccinated mares, and ranged from 1:8 to 1:32.

  15. nfrared Spectral Analysis of a Blood Serum as a Reflection of the Metabolic Process Disturbance Level at Infectious Pathology in Children

    Directory of Open Access Journals (Sweden)

    Krasnov V.V.

    2009-06-01

    Full Text Available The aim of work is a detection of the IR-spectroscopic analysis integrative value correlation with a toxicosis expression degree, stipulated by the metabolic disturbance level. Materials and Methods. 183 children with different clinical variants of diphtheria, angina, infectious mononucleosis and 30 healthy children at the age of 1 to 14 years were observed. The dried blood serum, prepared for the IR-spectral analysis, was investigated. Results and discussion. The differences of the spectral analysis conventional mathematical symbol (parameter numerical meanings at different diseases in children depending on a degree of intoxication expression, characteristic of each disease, as well as on their meanings in healthy children, are revealed. Besides, the substantional differences of numerical meanings are marked at different outcomes of the diphtheria critical forms (the survived and died children. A correlation between the blood serum IR-spectral analysis alterations and a metabolism disturbance level at infectious pathology, defined by the intoxication expression degree, is revealed. It is presumed, that the blood serum IR-spectra reflect a level of metabolic disturbances in the organism, appearing under the influence of infectious agent. These alterations are not specific for any separate disease and reflect the common regularities of the infectious process pathogenesis.

  16. Protein electrophoresis - serum

    Science.gov (United States)

    ... this page: //medlineplus.gov/ency/article/003540.htm Protein electrophoresis - serum To use the sharing features on ... JavaScript. This lab test measures the types of protein in the fluid (serum) part of a blood ...

  17. Effect of low level Doses of fast neutrons on the toxicity of snake venom through measuring some biophysical properties of blood serum of rats

    International Nuclear Information System (INIS)

    This study was conducted to investigate the effect of low level doses of fission neutrons from Cf 252 source on sublethal doses (low medium) of snake venom cerastes cerastes by injecting albino eats with unirradiated or irradiated venom and measuring the biophysical alterations in the blood serum of the rats. The biophysical properties of the total serum proteins were studied through measuring their dielectric relaxation and the electric conductivity in the frequency range 0.1→5 MHz at 4 degree C. The absorption spectra of the extracted total serum protein were also measured. The results indicated that there are pronounced changes in the molecular constructions of the total serum protein such as the molecular radii, shape, the relaxation time and dielectric increment for the rats injected with unirradiated venom but for the rats injected with irradiated venom (3x108 n/cm2) corresponding values approach the control value. These changes in the molecular constructions of the total serum protein indicate changes in its biochemical properties. This fact was revealed in a previous work, where the irradiation with the fast neutrons were found to decrease the toxicity of the venom

  18. Detection of Hepatitis B Virus (HBV) in Blood Serum By Means of PCR (Polymerase Chain Reaction) Technique

    International Nuclear Information System (INIS)

    Research for detecting the presence of HBV DNA in serum with PCR technique by using two pairs of oligonucleotide primers, has been carried out. Ten serum consisted of 5 HBsAg positive serum, I HBsAg weak positive serum, 3 HBsAg negative serum, and I sampel with negative HBV DNA as a previous PCR product trom another laboratory, were used to purify and to extract the DNA of virus, the sample pretreatment was done with Boom method. The two pairs of primers used for the- PCR process, were PC1 and PC2 and P1 and P2. The amplification process by means of PC1 and PC2 primer was carried out with two treatments, l.a. and l.b treatments of 5 HBsAg positive serum samples, 3 were positive for HBV DNA by PCR test with l.a. treatment. The PCR test by means of either the same primer but different treaunent (l.b treatment) or different pair of primer (pI and P2 pimer), revealed the presence of HBV DNA in all of HBsAg serum mentioned above of HBsAg negative Seruln, I serum was positive for HBV DNA and it was an amplification product of PCR test by using PI and P2 primer. The amplification products of PCR processwith either l.b treatment or PI and P2 primer, showed the positive results for I HBV positive serum as a previous PCR product trom another laboratory. All of the PCR test in this research provided the negative HBV DNA result in the HBsAg weak positive serum. The DNA amplification process by means of PI and P2 primer was more sensitive compared with PC I and PC2 primer

  19. Identification of muscle-specific microRNAs in serum of muscular dystrophy animal models: promising novel blood-based markers for muscular dystrophy.

    Directory of Open Access Journals (Sweden)

    Hideya Mizuno

    Full Text Available Duchenne muscular dystrophy (DMD is a lethal X-linked disorder caused by mutations in the dystrophin gene, which encodes a cytoskeletal protein, dystrophin. Creatine kinase (CK is generally used as a blood-based biomarker for muscular disease including DMD, but it is not always reliable since it is easily affected by stress to the body, such as exercise. Therefore, more reliable biomarkers of muscular dystrophy have long been desired. MicroRNAs (miRNAs are small, ∼22 nucleotide, noncoding RNAs which play important roles in the regulation of gene expression at the post-transcriptional level. Recently, it has been reported that miRNAs exist in blood. In this study, we hypothesized that the expression levels of specific serum circulating miRNAs may be useful to monitor the pathological progression of muscular diseases, and therefore explored the possibility of these miRNAs as new biomarkers for muscular diseases. To confirm this hypothesis, we quantified the expression levels of miRNAs in serum of the dystrophin-deficient muscular dystrophy mouse model, mdx, and the canine X-linked muscular dystrophy in Japan dog model (CXMD(J, by real-time PCR. We found that the serum levels of several muscle-specific miRNAs (miR-1, miR-133a and miR-206 are increased in both mdx and CXMD(J. Interestingly, unlike CK levels, expression levels of these miRNAs in mdx serum are little influenced by exercise using treadmill. These results suggest that serum miRNAs are useful and reliable biomarkers for muscular dystrophy.

  20. Cysticercus antibodies and antigens in serum from blood donors from Pondicherry, India Antígenos e anticorpos de Cysticercus em soro de doadores de sangue de Pondicherry, Índia

    OpenAIRE

    Subhash Chandra Parija; N. Balamurungan; Priyadarshi Soumyaranjan Sahu; S.P. Subbaiah

    2005-01-01

    The aim of the present study was to screen the serum of blood donors, which are apparently healthy and residing in Pondicherry or its neighboring districts of Tamil Nadu State, for specific detection of Cysticercus antigens and antibodies. A total of 216 blood samples were collected from blood donors at the Central Blood Bank, JIPMER Hospital, Pondicherry, India during January and February 2004. Enzyme-linked immunosorbent assay (ELISA) was used to demonstrate anti-Cysticercus antibodies and ...

  1. Comparative analysis of the concentration of matrix metalloproteinases in the blood serum before and after surgical treatment of breast cancer

    OpenAIRE

    Shek Dmitrii Leonidovich

    2015-01-01

    An immunosorbent identification and comparative analysis of the content of matrix metalloproteinases 2, 7 and 9 in the serum of patients with breast cancer (BC) before and two weeks after the surgical removal of the tumor.

  2. Effect of supplements: Probiotics and probiotic plus honey on blood cell counts and serum IgA in patients receiving pelvic radiotherapy

    Science.gov (United States)

    Mansouri-Tehrani, Hajar-Alsadat; Rabbani-Khorasgani, Mohammad; Hosseini, Sayyed Mohsen; Mokarian, Fariborz; Mahdavi, Hoda; Roayaei, Mahnaz

    2015-01-01

    Background: Radiotherapy is frequently used in treatment approaches of pelvic malignancies. Nevertheless, it has some known systemic effects on blood cells and the immune system that possibly results in their susceptibility to infection. Probiotics are live microbial food ingredients that provide a health advantage to the consumer. Honey has prebiotic properties. The aim of this clinical trial was to investigate probable effects of probiotic or probiotics plus honey on blood cell counts and serum IgA levels in patients receiving pelvic radiotherapy. Materials and Methods: Sixty-seven adult patients with pelvic cancer were enrolled. Patients were randomized to receive either: (1) Probiotic capsules (including: Lactobacillus casei, Lactobacillus acidophilus, Lactobacillus rhamnosus, Lactobacillus bulgaricus, Bifidobacterium breve, Bifidobacterium longum, and Streptococcus thermophiles) (n = 22), (2) probiotic capsules plus honey (n = 21) or (3) placebo capsules (n = 24) all for 6 weeks. Blood and serum samples were collected for one week before radiotherapy and 24-72 h after the end of radiotherapy. Results: White blood cells (WBC), red blood cells (RBC), platelet counts, and serum IgA level were not significantly changed in patients taking probiotic (alone or plus honey) during pelvic radiotherapy. The mean decrease in RBC count was 0.52, 0.18, and 0.23 × 106 cells/μL, WBC count was 2.3, 1.21, and 1.34 × 103 cells/μL and platelet count was, 57.6, 53.3, and 66.35 × 103 cells/μL for the probiotic, probiotic plus honey, and placebo groups, respectively. The mean decrease of serum IgA was 22.53, 29.94, and 40.73 mg/dL for the probiotic, probiotic plus honey, and placebo groups, respectively. Conclusion: The observed nonsignificant effect of probiotics may be in favor of local effects of this product in the gut rather than systemic effects, however, as a trend toward a benefit was indicated, further studies are necessary in order to extract effects of probiotics or

  3. Serum free hemoglobin test

    Science.gov (United States)

    Blood hemoglobin; Serum hemoglobin ... Hemoglobin (Hb) is the main component of red blood cells. It is a protein that carries oxygen. ... people may contain up to 5 mg/dL hemoglobin. Normal value ranges may vary slightly among different ...

  4. No effect of human serum and erythrocytes enriched in n-3 fatty acids by oral intake on Plasmodium falciparum blood stage parasites in vitro

    DEFF Research Database (Denmark)

    Abu-Zeid, Y A; Hansen, H S; Jakobsen, P H;

    1993-01-01

    acid (EPA, 20:5n-3) of 3.5 g/d and docosahexaenoic acid (DHA, 22:6n-3) of 2.5 g/d and 24 mg/d of total tocopherol. Post-intake fish oil serum (post-s) and erythrocytes (post-e) were tested in vitro for inhibitory activity against blood stages of P. falciparum compared with pre-intake serum (pre-s) and...... pre-intake erythrocyte (pre-e). Also the effect of EPA and arachidonic acid (AA, 20:4n-6) on the erythrocytic growth of P. falciparum was tested using in vitro assays. The results show that both post-s and post-e had no antimalarial activity on P. falciparum. No differential antimalarial effect was...

  5. Rapid quantification of conjugated and unconjugated bile acids and C27 precursors in dried blood spots and small volumes of serum[S

    OpenAIRE

    Janzen, N; Sander, S.; Terhardt, M.; Das, A.M; Sass, J.O.; Kraetzner, R.; Rosewich, H.; Peter, M.; Sander, J.

    2010-01-01

    The aim of the study was to develop a method for fast and reliable diagnosis of peroxisomal diseases and to facilitate differential diagnosis of cholestatic hepatopathy. For the quantification of bile acids and their conjugates as well as C27 precursors di- and trihydroxycholestanoic acid (DHCA, THCA), in small pediatric blood samples we combined HPLC separation on a reverse-phase C18 column with ESI-MS/MS analysis in the negative ion mode. Analysis was done with good precision (CV 3,7%–11.1%...

  6. Study of the Effect of Garlic on Serum Lipids and Blood Glucose Levels in Type 2 Diabetic Patients

    OpenAIRE

    M Afkhami - Ardekani; AR Kamali - Ardakani

    2005-01-01

    Introduction: Hyperlipidemia and diabetes are common risk factors for ischemic heart disease, which is the main cause of mortality in diabetic patients. Strict control of blood glucose and other risk factors in diabetics has led to prevention of complications. Garlic has received particular attention for control of blood glucose and decrease in blood lipid levels. At present, several studies have been carried out in order to prove advantages of garlic. Methods: In this study, effects of garsi...

  7. Experimental infection of commercial layers with wild or attenuated Salmonella Gallinarum mutant strains: anatomic pathology, total blood cell count and serum protein levels

    Directory of Open Access Journals (Sweden)

    KO Garcia

    2013-06-01

    Full Text Available The aim of the present study was to comparatively evaluate hemogram, blood serum components and anatomopathologic alterations in commercial layers experimentally challenged with an attenuated vaccine candidate strain (SG∆cobS∆cbiA and other two pathogenic strains (SGDcobS and SGNalr of Gallinarum (SG. In total, 280 commercial layers were randomly divided into 4 groups (G1, G2, G3 and G4. At five days of age, birds from groups G1 received approximately 107 colony forming units (CFU of SGDcobS; meanwhile birds from group G2 and G3 received the same dose of SGNalr and SG∆cobS∆cbiA, respectively. Birds from G4 were not infected. At 24 hours before (DBI and 24 hours after (1 DAI, and three (3 DAI, five (5 DAI, seven (7 DAI ten (10 DAI, and fifteen (15 DAI days after the infection, 10 birds of each group were humanely killed and blood samples collected to hematological and serum tests. Samples of liver, spleen, thymus, bursa of Fabricius, kidney and heart were also collected for the histological examination. Birds inoculated with SGDcobS and SGNalr showed similar alterations in hemogram, blood serum components and anatomopathologic exams. On the other hand, the exams of birds inoculated with SG∆cobS∆cbiA strain were similar to those of the uninfected birds. However, changes could be noticed in levels of uric acid and cholesterol during the course of the infection of birds from G3. Decrease in levels of light IgG 3 DAI was also observed in birds from this group. Pyknosis in kidney cells was a microscopic alteration found in birds from G3. Further studies must be done to verify if these alterations will not interfere in the performance of the vaccinate birds with SG∆cobS∆cbiA strain.

  8. Chemical speciation of arsenic species in human blood serum by liquid chromatography using a phosphatidylcholine-coated ODS column with detection by ICP-MS

    International Nuclear Information System (INIS)

    Chemical speciation of arsenic species in human blood serum was performed by high-performance liquid chromatography/inductively coupled plasma mass spectrometry (HPLC/ICP-MS) with direct sample injection, where an octadecylsilyl silica (ODS) column coated with phosphatidylcholine (PC) (hereafter known as 'PC-coated ODS column') was used as the separation column. In arsenic species analysis, a citrate buffer solution (pH 4.0) was used as the mobile phase, in which the following reagents were added: sodium 1-dodecanesulfonate (SDS), tetramethylammonium hydroxide (TMAH), which are ion-pair reagents to separate inorganic and organic arsenic species, and 3-[(3-cholamidopropyl)-dimethylammonio]-1-propane sulfonate (CHAPS), which is a protein-solubilizing agent to prevent adsorption of proteins on the column. As a result of optimization, five representative arsenic species spiked in human blood serum reference material could be separated from each other within 5 min on the PC-coated ODS column by elution with a 5 mM citrate buffer (pH 4.0) containing 5 mM SDS, 5 mM THAH, and 0.2 mM CHAPS. The detection limits obtained by ICP-MS were 3.1, 2.7, 4.5, 2.5, and 2.5 ng of As g-1 for arsenate (iAsV), arsenite (iAsIII), monomethylarsonic acid (MMA), dimethylarsenic acid (DMA), and arsenobetaine (AB), respectively, when the injection volume was 20 μL. The present separation system was also applied to speciation analysis of arsenic species in human blood serum collected from a leukemia patient after therapeutic treatment with arsenic. (author)

  9. Assessment risk of osteoporosis in Chinese people: relationship among body mass index, serum lipid profiles, blood glucose, and bone mineral density

    Science.gov (United States)

    Cui, Rongtao; Zhou, Lin; Li, Zuohong; Li, Qing; Qi, Zhiming; Zhang, Junyong

    2016-01-01

    Objective The aim of our study was to investigate the relationship among age, sex, body mass index (BMI), serum lipid profiles, blood glucose (BG), and bone mineral density (BMD), making an assessment of the risk of osteoporosis. Materials and methods A total of 1,035 male and 3,953 female healthy volunteers (aged 41–95 years) were recruited by an open invitation. The basic information, including age, sex, height, weight, waistline, hipline, menstrual cycle, and medical history, were collected by a questionnaire survey and physical examination. Serum lipid profiles, BG, postprandial blood glucose, and glycosylated hemoglobin were obtained after 12 hours fasting. BMD in lumbar spine was measured by dual-energy X-ray absorptiometry scanning. Results The age-adjusted BMD in females was significantly lower than in males. With aging, greater differences of BMD distribution exist in elderly females than in males (P7.0 mmol/L was lower than in people with BG of ≤7.0 mmol/L (P<0.001). People with serum high-density lipoprotein cholesterol levels of ≥1.56 mmol/L had a greater prevalence of osteoporosis compared with high-density lipoprotein cholesterol ≤1.55 mmol/L. Logistic regression with odds ratios showed that no association was found among total cholesterol, triglyceride, low-density lipoprotein cholesterol, glycosylated hemoglobin, postprandial blood glucose and BMD. Conclusion The present study further confirmed that factors such as age, sex, weight, BMI, high-density lipoprotein cholesterol, and diabetes are significant predictors of osteoporosis in the Chinese people. PMID:27445467

  10. Relationship between changes of blood HBV-DNA viral load and serum liver fibrosis markers (HA, LN, IV-C, PCIII) levels in patients with chronic hepatitis B

    International Nuclear Information System (INIS)

    Objective: To investigate the relationship between changes of blood HBV DNA viral load and serum liver fibrosis markers levels in patients with chronic hepatitis B. Methods: In 2002, 20 patients with hepatitis B were divided into two groups: Group A treated with antiviral ageat (n=10), Group B not treated.Five years later (2007) the blood viral load (with FQ-PCR) and serum levels of hepatic fibrosis markers (with RIA) were determined in these patients. Results: The average log viral load in serum in the tow groups were 3.56 ± 1.12 (treated group) and 7.76 ± 1.23 respectively with significant difference (P<0.05). In 2002, serum liver fibrosis markers (HA, LN, IV-C, PCIII) levels were about the same in the two groups were (82.72 ± 30.62μg/ml, 71.18 ± 26.71μg/ml, 93.77 ± 69.87μg/ml, 91.4 ± 18.64μg/ml and 79.32 ± 31.34μg/ml, 70.25 ± 28.23)μg/ml, 90.35 ± 67.81μg/ml, 85.77 ± 20.56μg/ml respectively). In 2007, in the treated patients, serum liver fibrosis markers HA, LN, IV-C, PCIII levels were 85.72 ± 29.52μg/ml, 70.18 ± 25.4μg/ml, 94.2 ± 70.92μg/ml, 93.4 ± 19.32μg/ml respectively However, in the non-treated groups, the serum HA, LN, IV-C, PCIII levels were105.67 ± 28.54μg/ml, 97.75 ± 26.25μg/ml, 132 ± 72.13μg/ml, 120.72 ± 19.87μg/ml, being significantly higher than those in the treated group (P<0.05). Conclusion: Effective decrease of the viral load might control the progress of hepatic fibrosis in patients with chronic hepatitis B. (authors)

  11. Post-exercise dynamics of serum amyloid A blood concentration in thoroughbred horses classified as injured and non-injured after the race.

    Science.gov (United States)

    Turlo, A; Cywinska, A; Czopowicz, M; Witkowski, L; Szarska, E; Winnicka, A

    2015-06-01

    The aim of this study was to evaluate serum amyloid A (SAA) concentration in horses with orthopedic injuries acquired during racing and in healthy ones after completing the race. Injuries of bone and tendon did not cause radical increase in SAA concentration observed in other inflammatory conditions. SAA concentration correlated positively with white blood cell count (WBC) on the 3rd-4th days after race being significantly higher in the injured horses than in the control group in that time. It was suggested that racing effort may cause increase in SAA level, more pronounced in horses manifesting clinical signs of orthopedic injury after the race. PMID:25933933

  12. Diagnostic value of serum procalcitonin levels in children with meningitis: a comparison with blood leukocyte count and C-reactive protein

    International Nuclear Information System (INIS)

    Objectives: To determine the level of serum procalcitonin, blood leukocyte count (TLC) and C-reactive protein (CRP) in children with bacterial and non bacterial meningitis and document their efficacy in differential diagnosis. Also described are procalcitonin levels variation during treatment. Methods: From March 2005 to February 2008, we evaluated 38 clinically suspected meningitis patients in the paediatric departments, Al-Jedaany Hospital, Jeddah, KSA, for Serum procalcitonin, CRP, TLC and Lumbar punctures and CSF analysis. Patients were classified into bacterial meningitis group I (18) and non bacterial meningitis group II (20). Results: Serum PCT levels were significantly higher in bacterial meningitis (BM) 9 mean 4.8 +- 3.85 ng/ml (2.9-11.6)) compared with non bacterial meningitis (NBM) (mean 0.38 +- 0.25 ng/ml(0.31-0.61)) P< 0.001). Mean of all CSF parameters, TLC (15,000 +- 2,900 cell/ml(BM) and 9,500 +-1,105 cell/ml(NBM))and CRP (20 +- 6.8 mg/l (BM) and 12.5 +-12.0 mg/l(NBM))showed a zone of overlapping between the two groups. There is a positive correlation between serum PCT, TLC and CRP in bacterial and non bacterial meningitis cases but this relation becomes highly significant with bacterial meningitis positive group. Day 3 and day 6 treatment serum PCT was less than on admission levels (P<0.001). Conclusion: PCT can be used in the early diagnosis of bacterial meningitis and may be a useful adjunct in differentiating bacterial and non bacterial meningitis than CRP or TLC and diminishing the value of lumbar puncture performed 48-72 hours after admission to assess treatment efficacy. (author)

  13. Blood Metal Concentrations of Manganese, Lead, and Cadmium in Relation to Serum Ferritin Levels in Ohio Residents

    Science.gov (United States)

    The objectives of this study were to assess fcrritin-specific profiles of blood metal concentrations such as manganese, lead, and cadmium and to evaluate whether ferritin may affect the behavior of the blood metals in relation to menstruation, menopause, or sex in Ohio residents....

  14. SDS-binding assay based on tyrosine fluorescence as a tool to determine binding properties of human serum albumin in blood plasma

    Science.gov (United States)

    Zhdanova, Nadezda; Shirshin, Evgeny; Fadeev, Victor; Priezzhev, Alexander

    2016-04-01

    Among all plasma proteins human serum albumin (HSA) is the most studied one as it is the main transport protein and can bind a wide variety of ligands especially fatty acids (FAs). The concentration of FAs bound to HSA in human blood plasma differs by three times under abnormal conditions (fasting, physical exercises or in case of social important diseases). In the present study a surfactant sodium dodecyl sulfate (SDS) was used to simulate FAs binding to HSA. It was shown that the increase of Tyr fluorescence of human blood plasma due to SDS addition can be completely explained by HSA-SDS complex formation. Binding parameters of SDS-HSA complex (average number of sites and apparent constant of complex formation) were determined from titration curves based on tyrosine (Tyr) fluorescence.

  15. Potent inhibition of replication of primary HIV type 1 isolates in peripheral blood lymphocytes by negatively charged human serum albumins

    NARCIS (Netherlands)

    Groenink, M; Swart, PJ; Broersen, S; Kuipers, M; Meijer, DKF; Schuitemaker, H

    1997-01-01

    We previously reported the antiviral capacity of human serum albumin (HSA), which was modified by the introduction of a single (Suc-HSA) or two carboxylic groups (Aco-HSA) per lysine residue, yielding strongly negatively charged polypeptides, Here we report the antiviral effect of these modified HSA

  16. Dose estimation of Tc-99m-DTPA-galactosyl-human serum albumin (Tc-99m-GSA) in the blood with nonlinear regression method

    International Nuclear Information System (INIS)

    Tc-99m-DTPA-galactosyl-human serum albumin (Tc-99m-GSA) was applied as an agent for receptor-binding hepatic scintigraphy. A single dose of 3-10 mg/185 MBq (5 mCi) Tc-99m-GSA per body was administered in four healthy volunteers and three patients. Time-activity curves for 60 (40) min after injection were obtained from the regions-of-interest over the heart and lung (background). Exponential regression curve was generated from the heart curve during 2-60 (40) min after background subtraction with nonlinear least squares method. Regression curve was well fitted to the heart curve and a significant correlation coefficient of 0.997±0.003 was obtained between these two curves. Absolute dose of GSA in the blood was decided on the assumption that extrapolated value of regression curve at time 0 represented the total injected dose. The absolute dose differences between sample blood and regression curve were 1.4-4.3% (mean). Nonlinear regression analysis is useful for estimating quantitative dose of GSA in blood without any blood samples. (author)

  17. Alteration of pulmonary blood flow in tetralogy of Fallot; Pre- and postoperative study with macroaggregates of [sup 99m]Tc-labeled human serum albumin

    Energy Technology Data Exchange (ETDEWEB)

    Hashimoto, Kazuhiro; Matsui, Michihiko; Kurosawa, Hiromi; Arai, Tatsuta (Jikei Univ., Tokyo (Japan). School of Medicine); Nakamura, Yuzuru

    1992-10-01

    The pulmonary blood distribution was examined in 17 patients with tetralogy of Fallot (TOF) pre and postoperatively with macroaggregates of [sup 99m]TC-labeled human serum albumin. Most of the patients with TOF demonstrated an abnormal preoperative distribution pattern. The abnormalities included not only an unbalanced distribution between the right and left lungs but also a maldistribution of peripheral vessels in each lung. The right/left lung counts ratio and pulmonary peripheral index (calculated in order to express the severity of peripheral maldistribution) correlated neither to the diameter nor the cross-sectional area of either right or left pulmonary arteries which were measured angiographically. Postoperatively, the pulmonary blood was shunted toward the developed side of the lung which further contributed to maldistribution of blood flow and unbalanced pulmonary growth. Since the patients with an unbalanced pulmonary blood distribution demonstrated a higher right ventricular pressure one year after the operation, a palliative operation facilitating the growth of the underdeveloped side of the lung might be considered as an effective procedure to precede intracardiac repair. (author).

  18. Dietary supplementation with dried chicory root triggers changes in the blood serum proteins engaged in the clotting process and the innate immune response in growing pigs.

    Science.gov (United States)

    Lepczynski, A; Herosimczyk, A; Ozgo, M; Skomial, J; Taciak, M; Barszcz, M; Berezecka, N

    2015-02-01

    The aim of the study was to characterize the systemic immune and metabolic alterations in the blood serum of growing pigs in response to a dietary supplementation with 4% of dried chicory roots. This was achieved by examining the influence of the experimental diet on serum protein changes especially these related with immunology and lipid metabolism. Serum proteins with the isoelectric point ranging from pH 3.0 to 10.0 were separated using high resolution two-dimensional electrophoresis. As a result, we found that experimental diet triggered significant changes in 37 protein spots. Of these, 14 were up-regulated, whereas 23 showed down-regulation. Of 37 significantly altered protein spots, 24 were successfully identified, representing 14 distinct gene products. Implementation of the dried chicory roots into the diet of growing pigs caused a significant down-regulation of apolipoprotein C-II complement component C6, C-reactive protein, CD14 antigen, C4b binding protein α and β chains, and fibrinogen. Piglets fed experimental diet had similar IgA, IgG and IgM concentrations, although the level of IgM tended to be lower compared to the control group. It is concluded that diet supplemented with 4% of dried chicory root may exert anti-inflammatory properties and affect lipid metabolism in growing pigs. PMID:25716964

  19. Effect of different types of stress on adrenal gland parameters and adrenal hormones in the blood serum of male Wistar rats

    Directory of Open Access Journals (Sweden)

    Adžić M.

    2009-01-01

    Full Text Available In the present study, we examined gross changes in the mass of whole adrenal glands and that of the adrenal cortex and medulla in mature male Wistar rats subjected to three different stress types: acute, chronic, and combined, i.e., chronic followed by acute stress. These parameters were correlated with adrenal activity as judged from serum levels of corticosterone and catecholamine, respectively, as well as with serum levels of ACTH and glucose. Under all three conditions, we observed bilaterally asymmetric and stress-type-independent hypertrophy of whole adrenals, as well as adrenal cortices and medullas. Under acute and combined stress, adrenal hypertrophy was followed by increase of adrenal hormones in the blood serum. However, under chronic stress, both cortical and medullar activities as judged from low or unaltered levels of the respective hormones and glucose were compromised and disconnected from the input signal of ACTH. Since all of the studied adrenal activities could be restored by subsequent acute stress, it is concluded that chronic isolation can be viewed as partly maladaptive stress with characteristics resembling stress resistance rather than the stress exhaustion stage of the general adaptation syndrome.

  20. Human adipose stromal cells expanded in human serum promote engraftment of human peripheral blood hematopoietic stem cells in NOD/SCID mice

    International Nuclear Information System (INIS)

    Human mesenchymal stem cells (hMSC), that have been reported to be present in bone marrow, adipose tissues, dermis, muscles, and peripheral blood, have the potential to differentiate along different lineages including those forming bone, cartilage, fat, muscle, and neuron. Therefore, hMSC are attractive candidates for cell and gene therapy. The optimal conditions for hMSC expansion require medium supplemented with fetal bovine serum (FBS). Some forms of cell therapy will involve multiple doses, raising a concern over immunological reactions caused by medium-derived FBS proteins. In this study, we cultured human adipose stromal cells (hADSC) and bone marrow stroma cells (HBMSC) in human serum (HS) during their isolation and expansion, and demonstrated that they maintain their proliferative capacity and ability for multilineage differentiation and promote engraftment of peripheral blood-derived CD34(+) cells mobilized from bone marrow in NOD/SCID mice. Our results indicate that hADSC and hBMSC cultured in HS can be used for clinical trials of cell and gene therapies, including promotion of engraftment after allogeneic HSC transplantation

  1. Flow injection spectrophotometric determination of vitamin E in Pharmaceuticals, milk powder and blood serum using potassium ferricyanide-Fe(Ⅲ) detection system

    Institute of Scientific and Technical Information of China (English)

    Saima

    2010-01-01

    A simple and sensitive flow injection spectrophotometric method is reported for the determination of vitamin E using potassium ferricyanide-Fe(Ⅲ detection system. In the presence of vitamin E, Fe(Ⅲ/ferricyanide reduces. The in situ reduced ions are then reacted with unreduced portion of ferricyanide/Fe(Ⅲ to make soluble Prussian blue, which is monitored at absorption wavelength of 735 nm. Linear calibration graph was obtained in the concentration range of 0.1-40 μg mL-1. The relative standard deviations (n = 4) were in the range of 1.1-3.6%, with limits of detection (3 s blank) of 0.04 μg mL-1. The proposed method allowed 12 injections h-1. The method is applied to determine vitamin E in Pharmaceuticals, infant milk and blood serum samples using hexane extraction with the recoveries in the range of 93 ± 3 to 97.5 ± 4%. The method is validated using certified reference materials SRM 968c for blood serum samples.

  2. Synthesis and study of cholosubstituted 4-aroyl pyrazolines and isoxazolines and their effects on inorganic ions in blood serum in albino rats

    Directory of Open Access Journals (Sweden)

    PRITHVISIGH R. RAJPUT

    2011-11-01

    Full Text Available Bhoyar AD, Vankhade GN, Rajput PR. 2011. Synthesis and study of cholosubstituted 4-aroyl pyrazolines and isoxazolines and their effects on inorganic ions in blood serum in albino rats. Nusantara Bioscience 3: 118-123. Condensation of 2-substitutied 3,5-dichloroacetophenones (2a-b obtained from the condensation of 2-hydroxy 3,5-dichloro-acetophenone (1 and benzoyl chloride were dissolved in NaOH, on treatment under Baker-Venkatraman transformation in presence of KOH with pyridine gives 1-(2-hydroxy-3,5-dichlorophenyl-3-substituted-1,3-propanediones (3a-b. Then converted into 3-aroyl-6,8-dichloroflavanones (4a-d by using different aromatic aldehyde in ethanol containing little piperidine. The condensation of (4a-d and phenylhydrazinehydrochloride, piperidine in DMF gives 3-(2-hydroxy3,5-dichlorophenyl-4-substitution-1-phenyl-2pyrazolines (5a-d and condensation of (4a-d and hydroxylamine-hydrochloride gives 3-(2-hydroxy-3,5-dichlorophenyl-4-aroyl-5-substituted isoxazolines (6a-d. The above compounds are screened for their activities and have been found to exhibit significant effects on inorganic ions in blood serum in albino rats.

  3. Effect Of L-Cysteine On Blood Picture And Some Serum Parameters In Rats Exposed To 2 Gauss Electro-Magnetic Field

    Directory of Open Access Journals (Sweden)

    Mervat Abdel-Rahman

    2004-12-01

    Full Text Available Objective: investigation of the bio-effects of exposure to 2 gauss electromagnetic field (EMF on blood elements, blood glucose, hepatocellular enzymes and bilirubin of mice and their possible modification by L-cysteine. Methods: the following groups were studied; (1 normal rats treated with saline; (2 normal rats treated with L-cysteine (18 mg/kg; (3 rats exposed to EMF for 21 days and treated with the vehicle (saline during the exposure period; (4 rats exposed to EMF for 21 days and treated with L-cysteine (18 mg/kg orally, 3 times per week during the exposure period; (5 rats exposed to EMF for 21 days and treated with the vehicle (saline during the exposure period and for 45 days after exposure; (6 rats exposed to EMF for 21 days and treated with L-cysteine (18 mg/kg orally, 3 times per week during the exposure period and for 45 days after exposure; Results: in rats exposed to low frequency EMF for 21 days (group2, marked increase in aspartate aminotransferase (AST and alanine aminotransferase (ALT activities in serum was observed. Plasma bilirubin level was raised. Meanwhile, significant decrease in plasma glucose levels occured after exposure to EMF. No significant changes were observed in haemoglobin level, red blood cells or total leucocytic count noted in rats exposed to EMF. The elevations in serum bilirubin, AST and ALT levels were reduced to near normal values in rats given L-cysteine druing the 21 days of exposure (group3. On the other hand, in rats examined 45 days after the end of the exposure period (group 3, no significant alterations were noted as regards bilirubin, AST, ALT and glucose levels in serum. Conclusions: these results suggest that (1 exposure to low frequency EMF of 50 Hz is associated with some degree of liver injury reflected in increased leakage of hepatoceular enzymes into plasma as well as an increase in serum bilirubin; (2 these alterations can be ameliorated by the administration of L-cysteine, as well as; (3

  4. Ferritin blood test

    Science.gov (United States)

    Serum ferritin level ... The amount of ferritin in the blood (serum ferritin level) is directly related to the amount of iron stored in your body. Iron is important for red blood cell production. Your doctor ...

  5. Levels of arsenic, mercury, cadmium, copper, lead, zinc and manganese in serum and whole blood of resident adults from mining and non-mining communities in Ghana.

    Science.gov (United States)

    Obiri, Samuel; Yeboah, Philip O; Osae, Shiloh; Adu-Kumi, Sam

    2016-08-01

    Human beings working or living near an industrial site where toxic chemicals such as As, Hg, Cd, Cu, Mn, Pb, Zn and or their compounds are used or indiscriminately discharged into the environment, are constantly exposed to such chemicals via ingestion (drinking or eating), dermal contact or inhalation (breathing). However, in developing countries such as Ghana, limited data on levels of the aforementioned chemicals in whole blood and serum of human beings as a result of exposure to the aforementioned chemicals from mining communities and non-mining communities is preventing effective policy formulation to protect human health. Hence, this study was undertaken to measure the levels of the aforementioned toxic chemicals in whole blood and serum of 300 resident adults from mining (Tarkwa Nsuaem Municipality Assembly (TNMA) and Prestea Huni Valley District (PHVD)) and non-mining (Cape Coast Metropolis) communities in Ghana, using neutron activation analysis (NAA). Blood samples were taken from 200 resident adults (105 males and 95 females) from mining and 100 resident adults (60 males and 40 males) from non-mining communities in the study area following the completion of an informed consent and the issuance of ethical clearance by the Ghana Health Service Ethical Committee. The mean concentrations for As, Hg, Cd, Cu, Mn, Pb and Zn in whole blood of residents from mining communities were as follows: 38 ± 320 μg/L, 63 ± 0.23 μg/L, 303 ± 117 μg/L, 3300 ± 953, 195 ± 90 μg/L, 28 ± 14 μg/L and 1405 ± 458 μg/L, respectively; while the levels of measured toxic chemicals in the serum of resident adults from mining communities were as follows: 65 ± 14 μg/L, 358 ± 22 μg/l, 134 ± 12 μg/L, 3590 ± 254 μg/L, 401 ± 113 μg/L, 58 ± 5.8 μg/L and 49 ± 31 μg/L, respectively, for As, Hg, Cd, Cu, Mn, Pb and Zn and were found to have exceeded the permissible WHO guideline values. PMID:27178291

  6. Usefulness of blood disappearance corrected hepatic uptake ratio (LHL/HH) as a hepatic functional index using 99mTc-galactosyl serum albumin

    International Nuclear Information System (INIS)

    The authors introduced blood disappearance corrected hepatic uptake ratio (LHL/HH) as an index of hepatic function using 185 MBq/3 mg of 99mTc-galactosyl serum albumin (GSA). Three ratio parameters, the hepatic uptake ratio (LHL15), blood disappearance ratio (HH15), and LHL15 divided by HH15 (LHL/HH) were evaluated with percent injected dose of 99mTc-GSA in blood at 15 min after injection (%ID15) and hepatic functional severity graded by Child-Turcotte criteria score. Seventy eight patients with chronic liver disease were studied. There was a poor linear correlation between the LHL15 and %ID15, because the LHL15 formed a plateau phase in the lower-value range for %ID15. In contrast, LHL/HH showed an excellent linear correlation with %ID15 in its whole range. In the comparison with the clinical functional severity, LHL/HH gave a more highly significant difference (p99mTc-GSA. (author)

  7. Peculiarities of Multiple Sclerosis Depending on Changes of Vitamin D Concentration in the Blood Serum of Patients

    OpenAIRE

    Orynchak, L. B.

    2015-01-01

    The objective of the research was to conduct a comparative analysis of 25(OH) vitamin D concentration in the serum of examined adults, patients with multiple sclerosis (MS), and the control group, depending on the clinical characteristics of the disease.Materials and methods of the research. The study involved 68 patients with MS, including 39 women and 29 men with confirmed MS diagnosis. The average age of patients was 37.7 ± 9.7, an average severity according to EDSS scale constituted 4.7 ±...

  8. Hypomethylation of serum blood clot DNA, but not plasma EDTA-blood cell pellet DNA, from vitamin B12-deficient subjects.

    Directory of Open Access Journals (Sweden)

    Eoin P Quinlivan

    Full Text Available Vitamin B12, a co-factor in methyl-group transfer, is important in maintaining DNA (deoxycytidine methylation. Using two independent assays we examined the effect of vitamin B12-deficiency (plasma vitamin B12<148 pmol/L on DNA methylation in women of childbearing age. Coagulated blood clot DNA from vitamin B12-deficient women had significantly (p<0.001 lower percentage deoxycytidine methylation (3.23±0.66%; n = 248 and greater [3 H]methyl-acceptance (42,859±9,699 cpm; n = 17 than DNA from B12-replete women (4.44±0.18%; n = 128 and 26,049±2,814 cpm; n = 11 [correlation between assays: r = -0.8538; p<0.001; n = 28]. In contrast, uncoagulated EDTA-blood cell pellet DNA from vitamin B12-deficient and B12-replete women exhibited similar percentage methylation (4.45±0.15%; n = 77 vs. 4.47±0.15%; n = 47 and [3 H]methyl-acceptance (27,378±4,094 cpm; n = 17 vs. 26,610±2,292 cpm; n = 11. Therefore, in simultaneously collected paired blood samples, vitamin B12-deficiency was associated with decreased DNA methylation only in coagulated samples. These findings highlight the importance of sample collection methods in epigenetic studies, and the potential impact biological processes can have on DNA methylation during collection.

  9. Influence of diet with kale on lipid peroxides and malondialdehyde levels in blood serum of laboratory rats over intoxication with paraquat

    Directory of Open Access Journals (Sweden)

    Elżbieta Sikora

    2013-03-01

    Full Text Available Background. Organism’s lipid peroxidation is one of the most often examined and known physiological process evoked by free radicals. It concerns oxidation reaction of unsaturated fatty acid and/or other lipids leading to lipid oxidation products (LOP, which as a result of further changes generate among others the malondialdehyde molecules. The aim of the work was an estimation if raw or cooked kale addition to rat’s diet infl uences antioxidant defense effi ciency in their organisms in comparison to rats fed with standard AIN-93G diet. Material and methods. The experiment was conducted with 36 Wistar strain, male rats over 21 days. The rats were divided into 3 groups (each 12 stuck which were fed with: standard diet AIN-93G (2 groups, AIN-93G diet with 10% addition of raw kale (2 groups, and AIN-93G with 10% addition of cooked lyophilised kale. The total content of polyphenols (FC method and antioxidant activity (ABTS+• were previously determined in raw and then in cooked kale. On the 20th day of experiment, half of rats (6 stuck of each kind of the diet were injected intraperitoneally by the solution of paraquat (PQ in physiological salt to evoke the oxidative stress. The next day animals were stunned and blood from their hearts was sampled. In the obtained serum, the levels of lipid oxidation products (LOP and malondialdehyde (MDA were assessed. Results. It was observed that in blood serum of rats fed with modifi ed diet with raw and cooked lyophilised kale addition the lipid oxides level was lower in comparison to control group fed with standard diet (p < 0.05. It was found that intoxication with paraquat caused growth of MDA and LOP levels in blood serum of all rats in comparison to not intoxicated groups but that growth was the lowest in group fed diet with cooked kale addition. Conclusion. Diet with kale, both raw and cooked, effi ciently inhibited the lipid peroxidation process in rats’ organisms, ongoing during natural metabolism

  10. Serum, plasma, and dried blood spot high sensitivity C-reactive protein enzyme immunoassay for population research

    OpenAIRE

    Brindle, Eleanor; FUJITA, MASAKO; Shofer, Jane; O’Connor, Kathleen A.

    2010-01-01

    C-reactive protein (CRP) is used as a biomarker of morbidity and mortality risk in studies of population health, and is essential to interpretation of several micronutrient biomarkers. There is thus need for a robust high sensitivity CRP (hsCRP) measurement method for large-scale, non-clinical studies. We developed an efficient, inexpensive assay suitable for quantifying CRP across the physiological range using any blood specimen type. The ELISA uses readily available monoclonal antibodies to...

  11. Correlation of serum C-reactive protein, white blood count and neutrophil percentage with histopathology findings in acute appendicitis

    OpenAIRE

    Xharra Shefki; Gashi-Luci Lumturije; Xharra Kumrije; Veselaj Fahredin; Bicaj Besnik; Sada Fatos; Krasniqi Avdyl

    2012-01-01

    Abstract Background Acute appendicitis is one of the most common surgical emergencies. Accurate diagnosis of acute appendicitis is based on careful history, physical examination, laboratory and imaging investigation. The aim of the study is to analyze the role of C-reactive protein (CRP), white blood count (WBC) and Neutrophil percentage (NP) in improving the accuracy of diagnosis of acute appendicitis and to compare it with the intraoperative assessment and histopathology findings. Materials...

  12. A Case of Hepatic Hemangioma Detected by Blood Pool with {sup 99m}Tc Human Serum Albumin

    Energy Technology Data Exchange (ETDEWEB)

    Yoon, Hwi Joong; Moon, Hee Bum; Kwon, In Soon; Choi, Kang Won; Kim, Chung Yong; Koh, Chang Soon [Seoul National University College of Medicine, Seoul (Korea, Republic of)

    1979-03-15

    A case of hepatic hemangioma is presented in which blood pool imaging by {sup 99m}Tc-HSA provided a definite diagnostic clue. Cavernous hemangiomas are the most common benign tumor of the liver. But the majority of them are asymptomatic and are found incidentally at laparotomy or autopsy. Selective hepatic arteriography has been the most helpful technique for the preoperative diagnosis. Recently radioisotope scan and ultrasonogram proved to be a new, definite and non- invasive diagnostic procedure.

  13. A case of hepatic hamangioma detected by blood pool scan with sup(99m)Tc human serum albumin

    International Nuclear Information System (INIS)

    A case of hepatic hemangioma is presented in which blood pool imaging by sup(99m)Tc-HSA provided a definite diagnostic clue. Cavernous hemangiomas are the most common benign tumor of the liver. But the majority of them are asymptomatic and are found incidentally at laparotomy or autopsy. Selective hepatic arteriography has been the most helpful technique for the preoperative diagnosis. Recently radioisotope scan and ultrasonogram proved to be a new, definite and non-invasive diagnostic procedure. (author)

  14. A Case of Hepatic Hemangioma Detected by Blood Pool with 99mTc Human Serum Albumin

    International Nuclear Information System (INIS)

    A case of hepatic hemangioma is presented in which blood pool imaging by 99mTc-HSA provided a definite diagnostic clue. Cavernous hemangiomas are the most common benign tumor of the liver. But the majority of them are asymptomatic and are found incidentally at laparotomy or autopsy. Selective hepatic arteriography has been the most helpful technique for the preoperative diagnosis. Recently radioisotope scan and ultrasonogram proved to be a new, definite and non- invasive diagnostic procedure.

  15. Experimental toxicity studies of cadmium in human blood serum in Jordan, using inductively coupled plasma (ICP) techniques

    International Nuclear Information System (INIS)

    A spectrometric inductively coupled plasma-emission atomic technique was used for the determination of cadmium in human blood samples. An organized system for the collection and handling of cadmium of human blood samples was applied. Also procedures for collection, preparation, analysis and quality control were suggested. The aim of this is to assist scientists world wide to produce comparable data which will be useful on regional, national and international scale. The guideline in this study is directed to (Cd) which ranged from non detectable to low measurable values. Limit of detection of ICP-EAS was in the range from 0.3 ppb to 40 ppb. Accuracy was evaluated by analysis of the certified reference materials, using a standard addition method. One hundred and three human blood samples were analyzed; the population for the study had ages between nineteen and twenty years. Samples were collected at a specific time during a typical study day and analyzed for cadmium. The results of analysis showed a low measurable and non-detectable concentrations of (Cd), which is within and below the limits recommended by World health Organization (WHO). (author)

  16. A defined, feeder-free, serum-free system to generate in vitro hematopoietic progenitors and differentiated blood cells from hESCs and hiPSCs.

    Directory of Open Access Journals (Sweden)

    Giorgia Salvagiotto

    Full Text Available Human ESC and iPSC are an attractive source of cells of high quantity and purity to be used to elucidate early human development processes, for drug discovery, and in clinical cell therapy applications. To efficiently differentiate pluripotent cells into a pure population of hematopoietic progenitors we have developed a new 2-dimensional, defined and highly efficient protocol that avoids the use of feeder cells, serum or embryoid body formation. Here we showed that a single matrix protein in combination with growth factors and a hypoxic environment is sufficient to generate from pluripotent cells hematopoietic progenitors capable of differentiating further in mature cell types of different lineages of the blood system. We tested the differentiation method using hESCs and 9 iPSC lines generated from different tissues. These data indicate the robustness of the protocol providing a valuable tool for the generation of clinical-grade hematopoietic cells from pluripotent cells.

  17. Rapid assessment of iron in blood plasma and serum by spectrophotometry with cloud-point extraction [version 1; referees: 2 approved

    Directory of Open Access Journals (Sweden)

    Tatyana Samarina

    2015-08-01

    Full Text Available Rapid photometric assessment of iron in blood plasma and serum by a simple procedure after the extraction of iron(II complex with 1-nitroso-2-naphthol in the micellar phase of a nonionic surfactant at the cloud point upon heating (pH range is 4.5–6.3 is proposed. The procedure trueness was verified using a standard reference protocol using bathophenanthroline. The advantages of the procedure are higher sensitivity than the reference protocol: the limit of detection is 0.03 μg/mL, the limit of quantitation is 0.1 μg/mL, the determination range is 0.1 – 2.8 μg/mL (RSD 0.02–0.10. Copper does not interfere with the iron assessment.

  18. Mixed reagents in multicomponent flow-injection analysis Simultaneous determination of iron and copper in blood serum with mixed bathocuproinedisulphonate and bathophenanthrolinedisulphonate or ferrozine.

    Science.gov (United States)

    Kubán, V; Gladilovich, D B; Sommer, L; Popov, P

    1989-04-01

    Mixtures of 0.6mM bathocuproinedisulphonate (BC) and 0.2mM bathophenanthrolinedisulphonate (BP) or 2mM BC and 0.2mM ferrozine (FZ) were used for a rapid determination of iron and copper in deproteinated blood serum in the presence of 0.1M formate buffer (pH 3.5), 10mM ascorbic acid and 0.3M trichloroacetic acid, by two variants of multicomponent FIA with a diode-array detector (MC-FIA). Merging zones MC-FIA is especially suitable for the determination of 0.7-33muM Fe and 0.4-35muMCu, for Cu:Fe concentration ratios from 10:1 to 1:10and with RSD individual chelates (at 2 or 3 wavelengths) also gave satisfactory results. PMID:18964739

  19. Alkaline phosphatase activity in blood serum of dogs exposed to a mixture of external γ-radiation and internal α-radiation

    International Nuclear Information System (INIS)

    Alkaline phosphatase activity in dog blood serum was studied for two years following separate and combined exposure to gamma radiation (6.45 to 51.6 mc/kg) and inhaled submicron 239Pu oxide containing 25% 241Am in chronically effective amounts (approx. 7-10 kBq/kg). Alkaline phosphatase activity was of an ondulatory nature and the significance of changes depended on the kind and the level of radiation as well as the time lapsed from the start of the expose. With the combined exposure to gamma and alpha radiation in the doses used no enhancement of the effec twaas noted as compared with the action of each factor applied separately

  20. Anti-inflammatory effect of cerivastatin in vascular injury independent of serum cholesterol and blood pressure lowering effects in mouse model

    Institute of Scientific and Technical Information of China (English)

    陈小东; 李震; 李建文

    2002-01-01

    Objective: To observe the anti-inflammatory effect of cerivastatin in a mouse model of vascular injury and its cholesterol-lowering effect. Methods: We developed a mouse model of vascular remodeling induced by polyethylene cuff placement and determined the anti-inflammatory effects of cerivastatin in wild mice. Cerivastatin was given by Alzet micro-osmotic minipumps implanted intraperitoneally at the same time as cuff placement at doses of 0.1 mg/kg, 0.5 mg/kg and 1 mg/kg per day, respectively for 2 weeks after cuff placement. The insufficient doses of Cerivastatin to lower serum cholesterol and systolic blood pressure through the neointimal formation and BrdU index were investigated in mouse femoral injury artery induced by cuff-placement. Results: There was a little change in serum cholesterol by the treatment with cerivastatin, the cross-sectional area of intima of injured femoral artery was significantly increased, the neointima formation was significantly increased by the cuff-induced vascular injury at day 14. The neointimal formation and BrdU index were inhibited in the 1 mg/kg cerivastatin, but not in the 0.1 mg/kg and 0.5 mg/kg cerivastatin. Furthermore, 1 mg/kg of cerivastatin significantly inhibited the expression of tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β) without lowering serum cholesterol.Conclusions: These results suggest that cerivastatin can inhibit vascular inflammation and the proliferation of vascular smooth muscle cells (VSMCs) through its lipid-lowering independent action. Such effects of cerivastatin may be an important mechanism, by which it prevents the development of atherosclerotic lesions.

  1. Spinal cord injury in rats: inability of nimodipine or anti-neutrophil serum to improve spinal cord blood flow or neurologic status

    International Nuclear Information System (INIS)

    The role of a calcium-mediated increase in vascular resistance and of vascular damage caused by polymorphonuclear leukocytes (PMNLs) in the development of neurologic deficit and disturbance of spinal cord circulation following spinal cord compression was studied in the rat. Spinal cord injury was induced by 5 min of compression with a load of 35 g on a 2.2 x 5.0 mm compression plate. This caused transient paraparesis. The rats received either the calcium receptor antagonist nimodipine or an anti-rat neutrophil serum (ANS). Nimodipine was infused i.v. for 4 h in an amount of 1.5 μg/kg/min starting 60 min after trauma. The number of circulating PMNLs was depleted by intraperiotoneal injection of an ANS raised in sheep given 12 h before trauma. This caused a reduction to about 2% of the pre-ANS value. Controls received saline or normal sheep serum. The motor performance was assessed daily on the inclined plane. On day one, the day after injury, the capacity angle had decreased from about 63 deg. preoperatively to close to 32 deg. in the experimental groups. There was then a slow improvement in both the control and experimental groups and on day 4 the capacity angle was close to 43 deg. in all 3 groups. Spinal cord blood flow, as measured with the 14C-iodoantipyrine autoradiography method, was similar in all groups on day 4. As neither the neurologic dysfunction nor the spinal cord blood flow was affected by post-trauma treatment with nimodipine or pretreatment with ANS, the possibility that calcium-mediated vasoconstriction or PMNLs play a role in the development of posttraumatic neuroligic disability was not supported by this study. (author)

  2. Simultaneous analysis of 22 antiepileptic drugs in postmortem blood, serum and plasma using LC-MS-MS with a focus on their role in forensic cases.

    Science.gov (United States)

    Deeb, Shaza; McKeown, Denise A; Torrance, Hazel J; Wylie, Fiona M; Logan, Barry K; Scott, Karen S

    2014-10-01

    In recent years, there has been a growth in reports of antiepileptic drugs (AEDs) being misused on their own or in combination with other drugs of abuse in a variety of toxicological case types such as drug abuse, suicide, overdose and drug facilitated crime. To our knowledge, there are no simultaneous quantification methods for the analysis of the most commonly encountered AEDs in postmortem whole blood and clinical plasma/serum samples at the same time. A simple, accurate and cost-effective liquid chromatography-tandem mass spectrometric (LC-MS-MS) method has been developed and validated for the simultaneous quantification of carbamazepine (CBZ) and its metabolite CBZ-10,11-epoxide, eslicarbazepine acetate, oxcarbazepine and S-licarbazepine as a metabolite, gabapentin, lacosamide, lamotrigine, levetiracetam, pregabalin, phenobarbital, phenytoin and its metabolite 5-(p-hydroxyphenyl)-5-phenylhydantoin, retigabine (ezogabine) and its metabolite N-acetyl retigabine, rufinamide, stiripentol, topiramate, tiagabine, valproic acid, vigabatrin and zonisamide in postmortem whole blood, serum and plasma which would be suitable for routine forensic toxicological analysis and therapeutic drug monitoring. All AEDs were detected and quantified within 17 min without endogenous interferences. The correlation coefficient (R(2)) was >0.995 for all AEDs with accuracy ranging from 90 to 113% and precision <13% for all analytes. The recovery ranged from 70 to 98%. No carryover was observed in a blank control injected after the highest standard and the matrix effect was acceptable and ranged from 90 to 120%. The method has been successfully verified using authentic case samples that had previously been quantified using different methods. PMID:25217536

  3. Determination of nickel in blood and serum samples of oropharyngeal cancer patients consumed smokeless tobacco products by cloud point extraction coupled with flame atomic absorption spectrometry.

    Science.gov (United States)

    Arain, Sadaf Sadia; Kazi, Tasneem Gul; Arain, Jamshed Bashir; Afridi, Hassan Imran; Kazi, Atif Gul; Nasreen, Syeda; Brahman, Kapil Dev

    2014-10-01

    Oropharyngeal cancer is a significant public health issue in the world. The incidence of oropharyngeal cancer has been increased among people who have habit of chewing smokeless tobacco (SLT) in Pakistan. The aim of present study was to evaluate the concentration of nickel (Ni) in biological samples (whole blood, serum) of oral (n = 95) and pharyngeal (n = 84) male cancer patients. For comparison purposes, the biological samples of healthy age-matched referents (n = 150), who consumed and did not consumed SLT products, were also analyzed for Ni levels. As the Ni level is very low in biological samples, a preconcentration procedure has been developed, prior to analysis of analyte by flame atomic absorption spectrometry (FAAS). The Ni in acid-digested biological samples was complexed with ammonium pyrrolidinedithio carbamate (APDC), and a resulted complex was extracted in a surfactant Triton X-114. Acidic ethanol was added to the surfactant-rich phase prior to its analysis by FAAS. The chemical variables, such as pH, amounts of reagents (APDC, Triton X-114), temperature, incubation time, and sample volume were optimized. The resulted data indicated that concentration of Ni was higher in blood and serum samples of cancer patients as compared to that of referents who have or have not consumed different SLT products (p = 0.012-0.001). It was also observed that healthy referents who consumed SLT products have two to threefold higher levels of Ni in both biological samples as compared to those who were not chewing SLT products (p < 0.01). PMID:24920259

  4. Responses of plasma cyclic AMP, serum immunoreactive insulin, C-peptide immunoreactivity and blood sugar levels to glucagon in patients with liver diseases.

    Directory of Open Access Journals (Sweden)

    Shimamura,Junnosuke

    1985-10-01

    Full Text Available Levels of plasma cyclic AMP, serum immunoreactive insulin (IRI, serum c-peptide immunoreactivity (CPR and blood sugar (BS were determined 0, 15, 30, 45 and 60 min after a glucagon injection (0.01 mg per kg body weight in normal controls, patients with acute hepatitis and liver cirrhosis. Plasma cyclic AMP responses to glucagon in liver disease patients varied widely in peak value, and only in patients with fulminant hepatitis and decompensated liver cirrhosis with poor prognosis was the response suppressed. The peak response of BS was found significantly later in liver cirrhosis patients than in normal controls. IRI and CPR responses to glucagon were lower in acute hepatitis patients than in normal controls and liver cirrhosis patients. IRI levels and their sum were also lower in acute hepatitis patients, although CPR levels were not significantly different. Thus, the ratio of the sum of CPR from 0 to 60 min to that of IRI was significantly higher in acute hepatitis, indicating impaired pancreatic secretion of insulin to glucagon stimulation as well as increased uptake of insulin by the liver in acute hepatitis.

  5. Seasonal changes in blood serum protein fractions and in activity of AspAT and AlAT in Arabian brood mares and their foals.

    Science.gov (United States)

    Gill, J; Jakubów, K; Kompanowska-Jezierska, E; Kott, A; Szumska, D

    1985-01-01

    In 34 pure breed Arabian horses divided into four groups (Gr. I--10 pregnant mares, Gr. II--7 barren mares, Gr. III--10 foals born in 1981, Gr. IV--7 foals born in 1982) seasonal changes in total blood serum protein, its electrophoretic fractions and the activity of AspAT and AlAT were studied. Seasonal cyclicity was found in all groups in the amount of total serum proteins, and alpha 2- and beta 1-globulin fractions. Cyclicity was found in the level of albumin and activity of AspAT in three groups, not Gr. II, and in gamma-globulin, not Gr. IV. beta 2-globulin and AlAT cyclicity was found in two groups and alpha 1-globulin cyclicity was found only in Gr. II. Out of nine indices studied, cyclicity was found in eight of them in pregnant mares, Gr. I; in seven in older foals, Gr. III; in six in the young foals, Gr. IV; and in five in barren mares, Gr. II. PMID:2864198

  6. Anti—inflammatory effect of cerivastatin in vascular injury independent of serum cholesterol and blood pressure lowering effects in mouse model

    Institute of Scientific and Technical Information of China (English)

    陈小东; 李震; 等

    2002-01-01

    Objective:To observe the anti-inflammatory effect of cerivastatin in a mouse model of vascular injury and its cholesterol-lowering effect.Methods:We developed a mouse model of vascular remodeling induced by polyethylene cuff placement and determined the anti-inflannatory effects of cerivastatin in wild mice.Cerivastatin was given by Alzet micro-osmotic minipumps implanted intraperitoneally at the same time as cuff placement at doses of 0.1mg/kg,0.5mg/kgand1mg/kgper day,respectively for 2weeks after cuff placement.The insufficient doses of Cerivastatin to lower serum cholesterol and systolic blood pressue through the neointimal formation and BrdUindex were investigated in mouse formation and BrdUindex were investigated in the 1mg/kg cerivastatin,but not in the 0.1mg/kgand0.5mg/kg cerivastatin,Furthermore,1mg/kg of cerivastatin significantly inhibited the expression of tumor necrosis factor-α(TNF-α)and interleukin-1β(IL-1β)without lowering serum cholesterol.Conclusions:These results suggest that cerivastatin can inhitit vascular inflammation and ahe proliferation of vascular smooth muscle cells(VSMCs)through its lipid-lowering independent action.Such effects of cerivastatin may be an important mechanism,by which it prevents the development of atherosclerotic lesions.

  7. Cytological comparison of gill chloride cells and blood serum ion concentrations in kutum (Rutilus frisii kutum spawners from brackish (Caspian Sea and fresh water (Khoshkrood River environments

    Directory of Open Access Journals (Sweden)

    Ghahremanzadeh Zahra

    2014-09-01

    Full Text Available The size and number of chloride cells and serum ion concentrations in kutum, Rutillus frisii kutum Nordman, from brackish (Caspian Sea and fresh water (Khoshkrood River environments were studied to gain a better understanding of osmoregulation in this species. Twenty mature kutum specimens were collected from the Caspian Sea (Anzali coasts, 8.49 ppt salinity and 12.4°C temperature and 20 specimens from Khoshkrood River (0.18 ppt salinity and 18°C temperature. Gill samples were analyzed histologically and concentrations of Na+, Cl- , K+, and Mg2+ ions were determined in the blood serum. Concentrations of Na+, Cl- , K+, and Mg2+ ions and osmotic pressure in mature kutum from brackish water were significantly higher than in specimens from fresh water. The average size and number of chloride cells in the fish from seawater were considerably larger than those from fresh water. The mean size of chloride cells was 6.89 ± 1.16 μm in brackish water samples and 5.1 ± 0.81 μm in river samples. The average number of chloride cells in brackish and river water samples were 16.92 and 6.57, respectively. The density and size of chloride cells increased with increases in salinity

  8. CORRELATIONS BETWEEN BLOOD PRESSURE AND BODY WEIGHT, SERUM LEPTIN IN HIGH CALORIE DIET-INDUCED OBESE RATS

    Institute of Scientific and Technical Information of China (English)

    2006-01-01

    Obesity is a complex,multifactorial diseasethat has beenshownto be anindependent risk factorfor the development of hypertension.Large epide-miological studies have confir med the associationbet ween body weight and blood pressure.Howev-er,the pathophysiological mechanis ms have notbeen completely understood.One problem in thestudy of the mechanis ms of obesity-related hyper-tensionis the lack of a suitable ani mal model.Thegenetic models of obesity[1]may or may not develophypertension or do not mi mic the c...

  9. Effects of different fibre sources and fat addition on cholesterol and cholesterol-related lipids in blood serum, bile and body tissues of growing pigs.

    Science.gov (United States)

    Kreuzer, M; Hanneken, H; Wittmann, M; Gerdemann, M M; Machmuller, A

    2002-04-01

    Knowledge is limited on the efficacy of hindgut-fermentable dietary fibre to reduce blood, bile and body tissue cholesterol levels. In three experiments with growing pigs the effects of different kinds and levels of bacterially fermentable fibre (BFS) on cholesterol metabolism were examined. Various diets calculated to have similar contents of metabolizable energy were supplied for complete fattening periods. In the first experiment, a stepwise increase from 12 to 20% BFS was performed by supplementing diets with fermentable fibre from sugar beet pulp (modelling hemicelluloses and pectin). Beet pulp, rye bran (modelling cellulose) and citrus pulp (pectin) were offered either independently or in a mixture in the second experiment. These diets were opposed to rations characterized in carbohydrate type by starch either mostly non-resistant (cassava) or partly resistant (maize) to small intestinal digestion. The third experiment was planned to explore the interactions of BFS from citrus pulp with fat either through additional coconut oil/palm kernel oil blend or full-fat soybeans. In all experiments the increase of the BFS content was associated with a constant (cellulose) or decreasing (hemicelluloses, pectin) dietary proportion of non-digestible fibre. In experiment 1 an inverse dose-response relationship between BFS content and cholesterol in blood serum and adipose tissue as well as bile acid concentration in bile was noted while muscle cholesterol did not respond. In experiment 2 the ingredients characterized by cellulose and hemicelluloses/pectin reduced cholesterol-related traits relative to the low-BFS-high-starch controls whereas, except in adipose tissue cholesterol content, the pectinous ingredient had the opposite effect. However, the changes in serum cholesterol mainly affected HDL and not LDL cholesterol. Adipose tissue cholesterol also was slightly lower with partly resistant starch compared to non-resistant starch in the diet. Experiment 3 showed that

  10. 血尿酸浓度与慢性心力衰竭患者的血糖血脂血压相关性研究%Analysis on the relationships between serum uric acid concentration and blood -sugar, blood -lipid, and blood- pressure in patients with chronic heart failure

    Institute of Scientific and Technical Information of China (English)

    宋健雄

    2011-01-01

    Objective To explore the relationships between serum uric acid(SUA) concentration and blood - sugar, blood lipid, and blood - pressure in patients with chronic heart failure. Methods Eighty -four patients suffering from chronic heart failure were used as the observation group and another 84 healthy people with normal heart function as the control group. The SUA, blood -pressure, fasting blood- glucose, blood- lipid including cholesterol, three acids glyceride, high -density lipo- protein, and low- density lipo- protein were determined in two groups. Results Compared with the control group, the patients with chronic heart failure have higher incidence in high SUA, high choles-terol, high blood - pressure, and abnormality in blood - sugar and blood - lipid. ( P < 0.05 ).Conclusions High SUA is relative to blood - sugar, blood - lipid, and blood - pressure in patients with chronic heart failure.%目的 探讨血尿酸浓度与慢性心力衰竭患者血糖、血脂、血压的关系.方法 选择84例慢性心力衰竭患者为观察组,另选84例心功能正常者为对照组,测定血尿酸、血压、空腹血糖、血脂,包括血浆总胆固醇、甘油三酯、高密度脂蛋白、低密度脂蛋白.结果 慢性心力衰竭组中高尿酸、高血压、血糖和血脂异常的发生率较高,与对照组比较差异有统计学意义(P<0.05).结论 高尿酸血症与慢性心力衰竭患者的血糖、血脂、血压水平密切相关.

  11. Immunoglobulin concentration in blood serum of postcolostral calves: Ratio between immunoglobulin level and appearance of enzootic pneumonia

    Directory of Open Access Journals (Sweden)

    Jonić Branko

    2007-01-01

    Full Text Available The timely supply of newborn calves with optimal quantities of colostrum has a key role in the process of immune protection in the early phase of their lives. Passively acquired antibodies can protect the digestive organs from infection caused by E.coli bacteria, and it seems also from the appearance of diseases of the respiratory tract. These examinations were performed on a cattle farm where bronchopneumonia was one of the most significant health problems, and a group of 39 calves were selected for the investigations. The calves were fed with their mothers’ colostrum after birth, and then with collective milk. Immunoglobulin concentration was determined in blood samples taken during the postcolostral period, with the method using zinc-sulphate. At the age of 40 days, the calves were administered a polyvalent inactivated vaccine, and revaccinated 20 days after that (Vibak, Veterinary Department Subotica. In 74.34% calves, the immunoglobulin G concentration ranged from 26 to 40 g/l. In 25.66% calves, the immunoglobulin concentration was lower, from 8 to 25 g/l. The calves found to have a lower concentration of immunoglobulin in blood contracted bronchopneumonia more frequently, and the outcome of the disease in some cases was mortality, even.

  12. High-Moisture Diet for Laboratory Rats: Complete Blood Counts, Serum Biochemical Values, and Intestinal Enzyme Activity

    Science.gov (United States)

    Battles, August H.; Knapka, Joseph T.; Stevens, Bruce R.; Lewis, Laura; Lang, Marie T.; Gruendel, Douglas J.

    1991-01-01

    Rats were fed an irradiated high-moisture diet (KSC-25) with or without access to a water bottle. Physiologic values were compared between these two groups and a group of rats fed a purified diet. Hematologic and serum biochemical values, urine specific gravity, and intestinal enzyme activities were determined from samples collected from the three groups of rats. Sprague Dawley rats (n=32) fed the irradiated high-moisture diet with or without a water bottle were the test animals. Rats (n=16) fed an irradiated purified diet and water provided via a water bottle were the control group. The purified diet formulation, modified AIN-76A, is a commonly used purified diet for laboratory rodents. All rats remained alert and healthy throughout the study. A comparison of the physiologic values of rats in this study with reported normal values indicated that all of the rats in the study were in good health. Significant differences (P less than 0.05) of the physiologic values from each rat group are reported.

  13. Genetic survey of an isolated community in Bali, Indonesia. I. Blood groups, serum proteins and hepatitis B serology.

    Science.gov (United States)

    Breguet, G; Ney, R; Grimm, W; Hope, S L; Kirk, R L; Blake, N M; Narendra, I B; Toha, A

    1982-01-01

    320 adults and children of an isolated community of Bali, Indonesia, have been tested for blood groups ABO, Rh, MNS, P, Lewis, Duffy, Kell, for haptoglobin and transferrin and for hepatitis B surface antigen and antibodies. Phenotype distribution and gene frequencies are given for the total population tested and for two subgroups representative of the inbred population of the isolate and of the non-inbred part of the population. Significant differences between the two subgroups show a clear genetic drift in the inbred population. The study brings biological support to the ethnological hypothesis of population migrations in this area. Tests for hepatitis B surface antigen reveal a lower prevalence of the disease than in most other south-east Asian populations. PMID:7068159

  14. Effect of enalapril on angiotensin II and serum nitric oxide in rats with high pulmonary blood flow

    International Nuclear Information System (INIS)

    Objective: To explore the therapeutic mechanism and effect of ACEI on pulmonary hypertension and pulmonary vascular structural remodeling induced by high pulmonary blood flow in rats. Methods: Twenty-three male SD rats were randomly divided into the control group, shunting group and shunting with enalapril group. Abdominal aorta and inferior vena cava shunting were produced in rats of shunting group and shunting with enalapril group. After 11-week shunting, pulmonary artery mean pressure (mPAP) of each rat was measured by using a right cardiac catheterization procedure. The ratio of right ventricular mass to body weight (RV/BW) and the ratio of right ventricular mass to left ventricular plus septal mass [RV/(LV+S)] were documented. Pulmonary vascular microstructure was measured. The ratio of vascular wall thickness to vascular external diameter (WT%) and the ratio of vascular wall area to total vascular area (WA%) were documented. Meanwhile, angiotensin II (Ang II) contents of lung tissue was measured by radioimmunoassay. The concentration of plasma nitric oxide (NO) was measured by spectrophotometry. Results: (1) After 11-week aortocaval shunting, mPAP, RV/BW and RV/(LV+S) were significantly increased in shunt rats as compared with those in the normal controls respectively. Muscularization of small pulmonary artery, WT% and WA% of pulmonary muscularized arteries were obviously increased in shunt rats compared with those in the controls. Meanwhile, in the lung tissue, Ang II contents also increased significantly, plasma NO concentration was significantly decreased. (2)However, mPAP, RV/BW and RV/(LV+S) were significantly decreased in rats in the shunting with enalapril group as compared with shunt rats. WT% and WA% of pulmonary muscularized arteries were obviously decreased in rats in the shunting with enalapril group compared with shunt rats. Meanwhile, in the lung tissue, Ang II contents also decreased significantly, plasma NO concentration was significantly

  15. Clinical trial of Cecropia obtusifolia and Marrubium vulgare leaf extracts on blood glucose and serum lipids in type 2 diabetics.

    Science.gov (United States)

    Herrera-Arellano, A; Aguilar-Santamaría, L; García-Hernández, B; Nicasio-Torres, P; Tortoriello, J

    2004-11-01

    Cecropia obtusifolia and Marrubium vulgare have been widely used in Mexican traditional medicine for the control of type 2 diabetes. In order to evaluate the clinical effect produced by the aqueous extract from these species on type 2 non-controlled diabetes mellitus, a total of 43 outpatients were included. Based on the European NIDDM (policy group) criteria, only patients with poor response to the conventional treatment were selected. All patients maintained their medical treatment and also received a prepared infusion of the dry leaves of the plant treatment for 21 days. In a double-blind manner, the patients were randomly grouped as follows: 22 patients were treated with C. obtusifolia and 21 with M. vulgare. The fasting blood glucose values were reduced by 15.25% on patients treated with C. obtusifolia, while cholesterol and triglycerides were decreased by 14.62% and 42.0%, respectively (ANOVA pvulgare, the plasma glucose level was reduced by 0.64% and cholesterol and triglycerides by 4.16% and 5.78%, respectively. When the results were compared between groups, significant differences in glucose and cholesterol diminution were found. The obtained results showed that the infusion prepared with the leaves of C. obtusifolia (containing 2.99+/-0.14mg of chlorogenic acid/g of dried plant) produced beneficial effects on carbohydrate and lipid metabolisms when it was administered as an adjunct on patients with type 2 diabetes with poor response to conventional medical treatment. PMID:15636168

  16. Study of changes in serum lipid profile and blood sugar level by perindopril and telmisartan during treatment of systemic hypertension

    Directory of Open Access Journals (Sweden)

    Manish Kumar

    2014-06-01

    Results: With perindopril initial means of TC, HDL, LDL, TGs, FBS, and PPBS in Groups A and B were 190.32, 49.76, 117.96, 165.04, 84.56, 122.60, and 188.80, 51.64, 118.52, 159.12, 93.92, 133.60, respectively. After 24 weeks, these values were 190.84, 50.68, 118.60, 163.84, 83.48, 120.20, and 190.96, 52.04, 118.28, 157.56, 93.96, 133.68, respectively (p > 0.05. With telmisartan, initial means of TC, HDL, LDL, TG, FBS, and PPBS in both groups were 188.08, 49.76, 118.84, 167.20, 83.72, 120.68, and 188.08, 46.88, 121.96, 167.84, 91.44, 131.72, respectively. After 24 weeks, these values in both groups were 189.36, 49.80, 120.04, 165.96, 82.60, 118.36 and 186.12, 45.28, 121.08, 167.72, 92.76, 129.56 respectively (p > 0.05. Conclusions: It concluded that both perindopril and telmisartan had not any significant adverse effects on plasma lipid profile and blood sugar level in both groups. [Int J Basic Clin Pharmacol 2014; 3(3.000: 454-459

  17. Effect of the hydroalcoholic extract and juice of Prunus divaricata fruit on blood glucose and serum lipids of normal and streptozotocin-induced diabetic rats.

    Science.gov (United States)

    Minaiyan, M; Ghannadi, A; Movahedian, A; Ramezanlou, P; Osooli, F S

    2014-01-01

    Prunus divaricata (Alloocheh) is a small tree cultivating in Iran, Middle East and central Asia. Prunus genus has many species with anti-oxidant, anti-hyperlipidemia and anti-hyperglycemia effects. In the present study the anti-diabetic and anti-hyperlipidemic effects of P. divaricata fruits were examined in normal and streptozotocin (STZ)-induced diabetic rats. Both groups, control and reference rats received normal saline and glibenclamide respectively. Test groups were treated with Prunus freeze dried juice (PFDJ, 200, 400, 800 mg/kg) and Prunus freeze dried extract (PFDE, 100, 200, 400 mg/kg) started at the 3(rd) day of the experiment and continued for 27 days thereafter. Weight changes of animals were checked periodically. Fasting blood glucose (FBG) level as well as serum triglyceride (TG), total cholesterol (TC), high density lipoprotein (HDL) and low density lipoprotein (LDL) cholesterol were determined. Different treatments had no significant effect on body weight increments of normal rats, while in diabetic rats, PFDJ (800 mg/kg) and PFDE (400 mg/kg) opposed with weight loss. In acute phase of experiment (0-8 h of 3(rd) day), none of tested fractions were effective in reducing FBG and serum lipids of normal rats. During the sub-acute phase (13(th) and 30(th) days) however, the greatest test doses of PFDJ (800 mg/kg) and PFDE (400 mg/kg) induced hypoglycema. In diabetic groups, PFDJ and PFDE, at all test doses, could diminish FBG during sub-acute phase of the experiment. In addition, PFDJ and PFDE at most examined doses could diminish TG significantly and they were also effective on cholesterol derivatives in different magnitude. PMID:26339257

  18. Hemograma e perfil bioquímico sérico, inclusive hemogasométrico, de bezerros infectados experimentalmente com Salmonella Dublin Blood cell counts and serum biochemical profile, including blood gas levels, in Salmonella Dublin-infected calves

    Directory of Open Access Journals (Sweden)

    D.G. Silva

    2010-04-01

    Full Text Available O objetivo do estudo foi avaliar o hemograma e o perfil bioquímico sérico, inclusive hemogasométrico, de bezerros infectados experimentalmente com Salmonella Dublin. Foram utilizados 12 bezerros sadios da raça Holandesa com 10 a 15 dias de idade, distribuídos aleatoriamente em dois grupos experimentais: grupo-controle (n= 6 e grupo infectado com 10(8UFC de Salmonella Dublin (n=6. Os bezerros foram submetidos ao exame físico diário, e as amostras de sangue foram coletadas minutos antes da inoculação (0h e 24, 48, 72, 96, 120, 144 e 168h após a inoculação. Além do hemograma e das análises hemogasométricas, foram mensuradas as atividades séricas das enzimas aspartato aminotransferase (AST, fosfatase alcalina (ALP, creatina cinase (CK, gamaglutamiltransferase (GGT e lactato desidrogenase (LDH, e os teores de albumina, bilirrubinas, cálcio total, cálcio ionizado, sódio, potássio, cloretos, creatinina, ferro, fibrinogênio, fósforo, glicose, magnésio, proteína totais e ureia. As principais alterações foram: redução das concentrações de albumina, ferro, glicose, magnésio e proteína total, aumento do teor de fibrinogênio, leucocitose e acidose metabólica e hiponatremia.The blood cell counts and biochemical profile, including blood gas levels, were evaluated, in Salmonella Dublin-infected calves. Twelve healthy 10 to 15-day old Holstein calves were randomly allotted into two groups: control (n=6 and group orally infected with 10(8 CFU Salmonella Dublin (n=6. The calves were submitted to physical examination and the blood samples were taken just before the inoculation (0h and at 24, 48, 72, 96, 120, 144, and 168h later. Besides, blood cell counts, blood gas levels, and the serum concentrations of aspartate aminotransferase (AST, alkaline phosphatase (ALP, creatine kinase (CK, gammaglutamyltransferase (GGT, lactate desidrogenase (LDH, albumin, bilirubin, total calcium, ionic calcium, sodium, potassium, chlorides, creatinine

  19. Correlation of serum C-reactive protein, white blood count and neutrophil percentage with histopathology findings in acute appendicitis

    Directory of Open Access Journals (Sweden)

    Xharra Shefki

    2012-08-01

    Full Text Available Abstract Background Acute appendicitis is one of the most common surgical emergencies. Accurate diagnosis of acute appendicitis is based on careful history, physical examination, laboratory and imaging investigation. The aim of the study is to analyze the role of C-reactive protein (CRP, white blood count (WBC and Neutrophil percentage (NP in improving the accuracy of diagnosis of acute appendicitis and to compare it with the intraoperative assessment and histopathology findings. Materials and methods This investigation was a prospective double blinded clinical study. The study was done on 173 patients surgically treated for acute appendicitis. The WBC, NP, and measurement of CRP were randomly collected pre-operatively from all involved patients. Macroscopic assessment was made from the operation. Appendectomy and a histopathology examination were performed on all patients. Gross description was compared with histopathology results and then correlated with CRP, WBC, and NP. Results The observational accuracy was 87,3%, as compared to histopathological accuracy which was 85.5% with a total of 173 patients that were operated on. The histopathology showed 25 (14.5% patients had normal appendices, and 148 (85.5% patients had acutely inflamed, gangrenous, or perforated appendicitis. 52% were male and 48% were female, with the age ranging from 5 to 59 with a median of 19.7. The gangrenous type was the most frequent (52.6%. The WBC was altered in 77.5% of the cases, NP in 72.3%, and C-reactive protein in 76.9% cases. In those with positive appendicitis, the CRP and WBC values were elevated in 126 patients (72.8%, whereas NP was higher than 75% in 117 patients (67.6%. Out of 106 patients with triple positive tests, 101 (95.2% had appendicitis. The sensitivity, specificity, and positive predictive values of the 3 tests in combination were 95.3%, 72.2%, and 95.3%, respectively. Conclusion The raised value of the CRP was directly related to the severity of

  20. Oral pathology follow-up by means of micro-Raman spectroscopy on tissue and blood serum samples: an application of wavelet and multivariate data analysis

    Science.gov (United States)

    Delfino, I.; Camerlingo, C.; Zenone, F.; Perna, G.; Capozzi, V.; Cirillo, N.; Gaeta, G. M.; De Mol, E.; Lepore, M.

    2009-02-01

    Pemphigus vulgaris (PV) is a potentially fatal autoimmune disease that cause blistering of the skin and oral cavity. It is characterized by disruption of cell-cell adhesion within the suprabasal layers of epithelium, a phenomenon termed acantholysis Patients with PV develop IgG autoantibodies against normal constituents of the intercellular substance of keratinocytes. The mechanisms by which such autoantibodies induce blisters are not clearly understood. The qualitative analysis of such effects provides important clues in the search for a specific diagnosis, and the quantitative analysis of biochemical abnormalities is important in measuring the extent of the disease process, designing therapy and evaluating the efficacy of treatment. Improved diagnostic techniques could permit the recognition of more subtle forms of disease and reveal incipient lesions clinically unapparent, so that progression of potentially severe forms could be reversed with appropriate treatment. In this paper, we report the results of our micro-Raman spectroscopy study on tissue and blood serum samples from ill, recovered and under therapy PV patients. The complexity of the differences among their characteristic Raman spectra has required a specific strategy to obtain reliable information on the illness stage of the patients For this purpose, wavelet techniques and advanced multivariate analysis methods have been developed and applied to the experimental Raman spectra. Promising results have been obtained.

  1. Increased miR-155 expression in peripheral blood mononuclear cells of primary immune thrombocytopenia patients was correlated with serum cytokine profiles.

    Science.gov (United States)

    Qian, Bao-Hua; Ye, Xin; Zhang, Lei; Sun, Yi; Zhang, Jian-Rong; Gu, Ming-Li; Qin, Qin; Chen, Jie; Deng, An-Mei

    2015-01-01

    We investigated the possible pathogenic role of a microRNA (miR-155) in primary immune thrombocytopenia (ITP). We used quantitative real-time PCR to determine the relative expression of miR-155 and SOCS1 (suppressor of cytokine signaling) mRNA in peripheral blood mononuclear cells (PBMCs) from 28 ITP patients and 28 healthy controls. Cytokine plasma levels were determined by ELISA. Possible associations between miR-155 levels and serum cytokine concentrations were assessed using Spearman or Pearson correlation analysis. Seven naive ITP patients were followed and the effects of medical treatment on miR-155 levels were assessed. Compared to healthy controls, ITP patients had increased miR-155 and decreased SOCS1 mRNA levels. ITP patients also had increased plasma IL-17A and decreased IL-4, IL-10 and TGF-β1 levels. miR-155 levels were negatively correlated with platelet counts, SOCS1 mRNA levels, and the plasma levels of IL-4, IL-10 and TGF-β1, but positively correlated with plasma IL-17A levels. Medical treatment for ITP decreased miR-155 levels. Thus, our results suggest that miR-155 might be involved in the pathogenesis of ITP by regulating cytokine profiles, which may be mediated by miR-155 targeting SOCS1. PMID:25413124

  2. Simplifying sample preparation using fabric phase sorptive extraction technique for the determination of benzodiazepines in blood serum by high-performance liquid chromatography.

    Science.gov (United States)

    Samanidou, Victoria; Kaltzi, Ioanna; Kabir, Abuzar; Furton, Kenneth G

    2016-06-01

    Fabric phase sorptive extraction (FPSE), a recently introduced novel sample preparation technology, has been evaluated for the extraction of benzodiazepines from human blood serum. FPSE utilizes a flexible fabric surface as the substrate platform for creating sol-gel hybrid organic-inorganic sorbent coatings. FPSE media can be introduced directly into the sample containing the target analyte(s), requiring no need for prior sample pretreatment or clean-up. Benzodiazepines were selected as model analytes because they represent one of the most widely used therapeutic drugs in psychiatry and are also amongst the most frequently encountered drugs in forensic toxicology. The chromatographic separation of target analytes was performed on a LiChroCART-LiChrospher®100 RP-18e (5 µm, 250 × 4 mm) analytical column, operated at room temperature. Ternary gradient elution was applied with a mobile phase that consisted of acetonitrile, methanol and ammonium acetate (0.05 M), which was delivered at a flow rate of 1.0 mL/min. Diode array detection was performed with monitoring at 240 nm. FPSE was performed using cellulose fabric extraction media coated with sol-gel poly(ethylene glycol) (sol-gel PEG). Absolute recovery values in the equilibrium state for the examined benzodiazepines were found to be 27% for bromazepam, 63% for lorazepam, 42 % for diazepam and 39% for alprazolam. Copyright © 2015 John Wiley & Sons, Ltd. PMID:26378746

  3. Facile fabrication of FeN nanoparticles/nitrogen-doped graphene core-shell hybrid and its use as a platform for NADH detection in human blood serum.

    Science.gov (United States)

    Balamurugan, Jayaraman; Thanh, Tran Duy; Kim, Nam Hoon; Lee, Joong Hee

    2016-09-15

    Herein, we present a novel strategy for the synthesis of an iron nitride nanoparticles-encapsulated nitrogen-doped graphene (FeN NPs/NG) core-shell hierarchical nanostructure to boost the electrochemical performance in a highly sensitive, selective, reproducible, and stable sensing platform for nicotinamide adenine dinucleotide (NADH). This core-shell hierarchical nanostructure provides an excellent conductive network for effective charge transfer and avoids the agglomeration and restacking of NG sheets, which provides better access to the electrode material for NADH oxidation. The FeN NPs/NG core-shell hierarchical nanostructure demonstrates direct and mediatorless responses to NADH oxidation at a low potential. This material displays a high sensitivity of 0.028μA/μMcm(2), a wide linear range from 0.4 to 718μM, and a detection limit of 25nM with a fast response time of less than 3s. The interferences from common interferents, such as glucose, uric acid, dopamine, and ascorbic acid, are negligible. The fabricated sensor was further tested for the determination of NADH in human blood serum. The resulting high sensitivity, excellent selectivity, outstanding stability, and good reproducibility make the proposed FeN NPs/NG core-shell hierarchical nanostructure as a promising candidate for biomedical applications. PMID:27104586

  4. Establishment of Elevated Serum Levels of IL-10, IL-8 and TNF-β as Potential Peripheral Blood Biomarkers in Tubercular Lymphadenitis: A Prospective Observational Cohort Study

    Science.gov (United States)

    Abhimanyu; Bose, Mridula; Varma-Basil, Mandira; Jain, Ashima; Sethi, Tavpritesh; Tiwari, Pradeep Kumar; Agrawal, Anurag; Banavaliker, Jayant Nagesh; Bhowmick, Kumar Tapas

    2016-01-01

    Background Tubercular lymphadenitis (TL) is the most common form of extra-pulmonary tuberculosis (TB) consisting about 15–20% of all TB cases. The currently available diagnostic modalities for (TL), are invasive and involve a high index of suspicion, having limited accuracy. We hypothesized that TL would have a distinct cytokine signature that would distinguish it from pulmonary TB (PTB), peripheral tubercular lymphadenopathy (LNTB), healthy controls (HC), other lymphadenopathies (LAP) and cancerous LAP. To assess this twelve cytokines (Tumor Necrosis Factor (TNF)—α, Interferon (IFN) -γ, Interleukin (IL)-2, IL-12, IL-18, IL-1β, IL-10, IL-6, IL-4, IL-1Receptor antagonist (IL-1Ra), IL-8 and TNF-β, which have a role in pathogenesis of tuberculosis, were tested as potential peripheral blood biomarkers to aid the diagnosis of TL when routine investigations prove to be of limited value. Methods and Findings A prospective observational cohort study carried out during 2010–2013. This was a multi-center study with three participating hospitals in Delhi, India where through random sampling cohorts were established. The subjects were above 15 years of age, HIV-negative with no predisposing ailments to TB (n = 338). The discovery cohort (n = 218) had LNTB (n = 50), PTB (n = 84) and HC (n = 84). The independent validation cohort (n = 120) composed of patients with cancerous LAP (n = 35), other LAP (n = 20) as well as with independent PTB (n = 30), LNTB (n = 15) and HC (n = 20). Eight out of twelve cytokines achieved statistical relevance upon evaluation by pairwise and ROC analysis. Further, variable selection using random forest backward elimination revealed six serum biosignatures including IL-12, IL-4, IL-6, IL-10, IL-8 and TNF-β as optimal for classifying the LNTB status of an individual. For the sake of clinical applicability we further selected a three analyte panel (IL-8, IL-10 and TNF-β) which was subjected to multinomial modeling in the independent

  5. 理想血压、正常高值血压和高血压与血尿酸相关性探讨%Association study of ideal blood pressure, high-normal blood pressure and hypertension with serum uric acid

    Institute of Scientific and Technical Information of China (English)

    王一军; 李琴芝

    2012-01-01

    目的 探讨理想血压、正常高值血压和高血压与血尿酸的相关性.方法 以理想血压、正常高值血压和高血压患者为研究对象,检测其血清尿酸水平.对检测结果进行比较和分析.结果 正常高值血压组的血尿酸水平显著高于理想血压组,而显著低于高血压组.正常高值血压组的高尿酸血症患者发病率显著高于理想血压组,而显著低于高血压组.血压与尿酸相关性分析显示:血尿酸与收缩压、舒张压均呈正相关.结论 血尿酸升高是高血压的一个独立危险因子.%Objective To investigate the relationship between ideal blood pressure,high-normal blood pressure and hypertension with serum uric acid.Methods In this study,the ideal blood pressure,high-normal blood pressure and hypertension patients were enrolled,the serum uric acid levels were detected,and the test results were compared and analyzed.Results The serum uric acid level of high-normal blood pressure group was significantly higher than that of ideal blood pressure group,but significantly lower than that of the hypertensive group.The incidence of hyperuricemia in high-normal blood pressure group was significantly higher than that in ideal blood pressure group and significantly lower than that in hypertensive group.Correlation between blood pressure and uric acid analysis showed:serum uric acid were positively related with systolic blood pressure and diastolic blood pressure.Conclusions The elevated serum uric acid level is an independent risk factor of hypertension.

  6. Effect of whole blood donation on serum iron and ferritin%定期无偿献血对献血者血清铁和铁蛋白的影响

    Institute of Scientific and Technical Information of China (English)

    亓法英; 赵孔波; 张春来; 于继徐

    2015-01-01

    目的:研究献血对血清铁含量的影响。方法收集76例无偿献血人员为献血组,83例健康人员(非献血人员)为对照组,采集空腹静脉血,进行血常规、血清铁和铁蛋白检查。结果献血组血清铁、红细胞和红细胞压积均在正常范围,血清铁蛋白和血红蛋白除1例外也在正常范围。献血组男性血清铁蛋白为(116.52±67.16)ng/mL,对照组男性为(186.39±103.15) ng/mL,两组比较差异有统计学意义(P <0.05);献血组女性血清铁蛋白为(66.2±47.62)ng/mL,对照组女性为(101.1±73.89) ng/mL,两组比较差异有统计学意义(P <0.05)。献血组血清铁与对照组比较差异无统计学意义(P >0.05)。结论献血组血清铁蛋白降低,但是并没有造成贫血。因此,献血可以减少体内的铁贮存量。%Objective To study the influence of blood donation serum iron levels.Methods 76 blood donors and 83 control subjects (who had never donated blood)were enrolled to this study.Venous blood samples were drawn for Hb,RBC,HCT,serum iron and ferritin analysis.Results Serum ferritin and Hb of enrolled donors except one were above the mandatory threshold.Serum iron,RBC and HCT of enrolled donors were all above the mandatory threshold.Furthermore,we found that serum ferritin of male blood donors(116.52±67.16 ng/mL)were significantly lower than that of control subjects (186.39±103.15 ng/mL),and serum ferritin of female blood donors(66.2±47.62 ng/mL)were significantly lower than that of control subjects(101.1±73.89 ng/mL). In contrast,there was no difference of serum iron between blood donors and control group.Conclusion Anemia had not occurred in enrolled donors,but serum ferritin level decreased.Thus,whole blood donation could diminish iron stores.

  7. Field Evaluation of Calypte’s AWARE™ Blood Serum Plasma (BSP) and Oral Mucosal Transudate (OMT) Rapid Tests for Detecting Antibodies to HIV-1 and 2 in Plasma and Oral Fluid

    OpenAIRE

    Alemnji, George A.; Ngulefac, Gisele A; Ndumbe, Peter M; Asonganyi, Tazoacha

    2009-01-01

    As programs to prevent and care for HIV-infected persons are scaled-up in Africa, there is the need for continuous evaluation of the performance of test kits that could best support these programs. The present study evaluated the sensitivity, specificity, ease of use, and cost of AWARE ™ Blood Serum Plasma (BSP) and Oral Mucosal Transudate (OMT) Rapid HIV-1/2 test kits using real-time and archived samples of HIV-infected persons from Cameroon. Matched whole blood and OMT specimens were collec...

  8. Functional Langerinhigh-Expressing Langerhans-like Cells Can Arise from CD14highCD16- Human Blood Monocytes in Serum-Free Condition.

    Science.gov (United States)

    Picarda, Gaëlle; Chéneau, Coraline; Humbert, Jean-Marc; Bériou, Gaëlle; Pilet, Paul; Martin, Jérôme; Duteille, Franck; Perrot, Pierre; Bellier-Waast, Frédérique; Heslan, Michèle; Haspot, Fabienne; Guillon, Fabien; Josien, Regis; Halary, Franck Albert

    2016-05-01

    Langerhans cells (LCs) are epithelial APCs that sense danger signals and in turn trigger specific immune responses. In steady-state, they participate in the maintenance of peripheral tolerance to self-antigens whereas under inflammation LCs efficiently trigger immune responses in secondary lymphoid organs. It has been demonstrated in mice that LC-deprived epithelia are rapidly replenished by short half-life langerin-expressing monocyte-derived LCs (MDLCs). These surrogate LCs are thought to be progressively replaced by langerin(high) LCs arising from self-renewing epithelial precursors of hematopoietic origin. How LCs arise from blood monocytes is not fully understood. Hence, we sought to characterize key factors that induce differentiation of langerin(high)-expressing monocyte-derived Langerhans-like cells. We identified GM-CSF and TGF-β1 as key cytokines to generate langerin(high)-expressing cells but only in serum-free conditions. These cells were shown to express the LC-specific TROP-2 and Axl surface markers and contained Birbeck granules. Surprisingly, E-cadherin was not spontaneously expressed by these cells but required a direct contact with keratinocytes to be stably induced. MDLCs induced stronger allogeneic T cell proliferations but released low amounts of inflammatory cytokines upon TLR stimulation compared with donor-paired monocyte-derived dendritic cells. Immature langerin(high) MDLCs were responsive to MIP-3β/CCL20 and CTAC/CCL27 chemokine stimulations. Finally, we demonstrated that those cells behaved as bona fide LCs when inserted in a three-dimensional rebuilt epithelium by becoming activated upon TLR or UV light stimulations. Collectively, these results prompt us to propose these langerin(high) MDLCs as a relevant model to address LC biology-related questions. PMID:27016604

  9. Addition of a sequence from α2-antiplasmin transforms human serum albumin into a blood clot component that speeds clot lysis

    Directory of Open Access Journals (Sweden)

    Gataiance Sharon

    2009-03-01

    Full Text Available Abstract Background The plasma protein α2-antiplasmin (α2AP is cross-linked to fibrin in blood clots by the transglutaminase factor XIIIa, and in that location retards clot lysis. Competition for this effect could be clinically useful in patients with thrombosis. We hypothesized that fusion of N-terminal portions of α2-antiplasmin to human serum albumin (HSA and production of the chimeric proteins in Pichia pastoris yeast would produce a stable and effective competitor protein. Results Fusion protein α2AP(13-42-HSA was efficiently secreted from transformed yeast and purified preparations contained within a mixed population the full-length intact form, while fusions with longer α2AP moieties were inefficiently secreted and/or degraded. The α2AP(13-42-HSA protein, but not recombinant HSA, was cross-linked to both chemical lysine donors and fibrin or fibrinogen by factor XIIIa, although with less rapid kinetics than native α2AP. Excess α2AP(13-42-HSA competed with α2AP for cross-linking to chemical lysine donors more effectively than a synthetic α2AP(13-42 peptide, and reduced the α2AP-dependent resistance to fibrinolysis of plasma clots equally effectively as the peptide. Native α2AP was found in in vivo clots in rabbits to a greater extent than α2AP(13-42, however. Conclusion In this first report of transfer of transglutamination substrate status from one plasma protein to another, fusion protein α2AP(13-42-HSA was shown to satisfy initial requirements for a long-lasting, well-tolerated competitive inhibitor of α2-antiplasmin predicted to act in a clot-localized manner.

  10. The effect of high level natural ionizing radiation on expression of PSA, CA19-9 and CEA tumor markers in blood serum of inhabitants of Ramsar, Iran

    International Nuclear Information System (INIS)

    Since several high level natural radiation areas (HLNRAs) exist on our planet, considerable attention has been drawn to health issues that may develop as the result of visiting or living in such places. City of Ramsar in Iran is an HNLRA, and is a tourist attraction mainly due to its hot spas. However, the growing awareness over its natural radiation sources has prompted widespread scientific investigation at national level. In this study, using an ELISA method, the level of expression of three tumor markers known as carcinoembryonic antigen (CEA), prostate-specific antigen (PSA) and carcino antigen 19-9 (CA19-9) in blood serum of 40 local men of Ramsar (subject group) was investigated and compared to 40 men from the city of Noshahr (control group). Noshahr was previously identified as a normal level natural radiation area (NLNRA) that is some 85 km far from Ramsar. According to statistical analysis, there was a significant difference in the levels of PSA and CA19-9 markers between the two groups (p < 0.001) with those of Ramsar being considerably higher. CEA level did not show any difference. Although some of the volunteers tested positive to the markers, they were in good health as confirmed by the physician. Moreover, the high number of positive markers in Noshahr was considerable. Therefore, future study is needed to further validate this result and to determine the level of positivity to tumor markers in both cities. -- Highlights: • Expression of three tumor markers was examined in 80 volunteers from Ramsar. • There was a significant difference in the levels of PSA and CA19-9 markers. • Some of the volunteers from the control city of Noshahr also tested positive. • Radiation might have caused an adaptive response in people of Ramsar. • Further study is necessary to re-confirm the positivity to tumor marker

  11. Study on the Changes in Enzyme and Insulin-like Growth Factor-1 Concentrations in Blood Serum and Growth Characteristics of Velvet Antler during the Antler Growth Period in Sika Deer (Cervus nippon).

    Science.gov (United States)

    Park, Jaehyun; Jeon, Byongtae; Kang, Sungki; Oh, Mirae; Kim, Myonghwa; Jang, Seyoung; Park, Pyojam; Kim, Sangwoo; Moon, Sangho

    2015-09-01

    This study was conducted to investigate changes in blood enzyme parameters and to evaluate the relationship between insulin-like growth factor-1 (IGF-1), antler growth and body weight during the antler growth of sika deer (Cervus nippon). Serum enzyme activity and IGF-1 concentrations were measured in blood samples collected from the jugular and femoral veins at regular intervals during the antler growth period. Blood samples were taken in the morning from fasted stags (n = 12) which were healthy and showed no clinical signs of disease. Alfalfa was available ad libitum and concentrates were given at 1% of body weight to all stags. The experimental diet was provided at 9 am with water available at all times. There were no significant differences in alkaline phosphatase, aspartate aminotransferase, and alanine aminotransferase during antler growth, but alkaline phosphatase concentrations increased with antler growth progression, and the highest alkaline phosphatase concentration was obtained 55 days after antler casting. Serum IGF-1 concentrations measured from blood samples taken from the jugular vein during antler growth, determined that levels of IGF-1 was associated with body weight and antler growth patterns. Serum IGF-1 concentrations were higher at the antler cutting date than other sampling dates. Antler length increased significantly during antler growth (p<0.001), and there was a similar trend to between right and left beams. Body weight increased with antler growth but was not significant. Consequently it appeared that serum alkaline phosphatase concentration was related to antler growth and both antler growth and body weight were associated positively with IGF-1 concentrations during antler growth. PMID:26194228

  12. The elements Se, Hg, Cr, Sb, Fe, Zn, Co, and Rb were investigated by neutron activation analysis in blood serum of leukemia patients and workers of metal coating industries

    International Nuclear Information System (INIS)

    Current universal interests in trace element studies are being spurred by our needs to determine trace element requirements and tolerance by organism, including relationships to animal and human health and disease, evaluate the potential bio magnification and bio toxicity of trace elements. The element Se, Hg, Cr, Sb, Fe, Zn, Co, and Rb were investigated in blood serum of 24 leukemia patients and 20 workers of metal coating of industries, using neutron activation analysis and gamma ray counting high purity germanium detector, it was possible to determine the above mentioned trace elements with great accuracy. The result of these investigations are described briefly. In the blood serum of leukemia patients, which were collected from Ali Asghar children Hospital, some increase of Fe and decrease of Se could be found, whereas the amount variation of other trace elements were negligible. The amount of Zn in the blood serum of these workers by comparison with 60 normal man indicates no change. Due to importance of trace elements in the human health we suggest that this investigation must be prolonged using more samples

  13. Alaska Steller sea lion pups blood serum chemistry and hematology values measured from 1998-06-01 to 2011-07-15 (NCEI Accession 0137994)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — These data were used for an analysis of Steller sea lion pup health and condition by Lander et al. (2013). Serum chemistry and hematological values were measured by...

  14. Determination of oligo elements, platinum and relation Zn/Cu in samples of blood serum and urine among oncological pediatric patients by means of Total Re flexion X-Ray Fluorescence

    International Nuclear Information System (INIS)

    Full text: An analytic methodology has been developed by means of Total Reflection X-Ray Fluorescence, for the determination of oligo elements, platinum in serum and urine, and the relation Zn/Cu in samples of blood serum of oncological pediatric patient. The technique allows the simultaneous determination of interesting elements with quantities of samples of microliters order, which is an advantage over other atomic spectroscopy techniques, because the analytic quality of the results is comparable. A simple procedure was developed by TXRF that allows the monitoring of levels of platinum in serum and urine, of drugs containing platinum, administered to patients under chemotherapy treatment, at the same time that the levels of Ca, K and the oligo elements Zn, Cu, Fe and Se are obtained. The procedure was also applied to the analysis of oligo elements in samples of serum and urine among oncological pediatric patient before the treatment and in a normal group (healthy) as reference. From the results obtained, it is deduced that there are significant differences among the total average levels of Zn and Cu among the groups with cancer and normal, as well as in the relation Zn/Cu, which was shown like a parameter with possible capacity for the cancer diagnosis in a more appropriated way than the oligo elements level, as it has already been reported before in the literature of researches on cancer in adults. All the details inherent to the developed analytic method will be object of discussion

  15. Diagnostic value of platelet derived growth factor-BB, transforming growth factor-β1,matrix metalloproteinase-1, and tissue inhibitor of matrix metalloproteinase-1 in serum and peripheral blood mononuclear cells for hepatic fibrosis

    Institute of Scientific and Technical Information of China (English)

    Bin-Bin Zhang; Wei-Min Cai; Hong-Lei Weng; Zhong-Rong Hu; Jun Lu; Min Zheng; Rong-Hua Liu

    2003-01-01

    AIM: Noninvasive diagnosis of hepatic fibrosis has become the focus because of the limited biopsy, especially in the surveillance of treatment and in screening hepatic fibrosis.Recently, regulatory elements involved in liver fibrosis, such as platelet derived growth factor-BB (PDGF-BB), transforming growth factor-β1 (TGF-β1), matrix metalloproteinase-1 (MMP-1), and tissue inhibitor of matrix metalloproteinase-1 (TIMP-1), have been studied extensively. To determine whether these factors or enzymes could be used as the indices for the diagnosis of hepatic fibrosis, we investigated them by means of receiver operating characteristic (ROC) curve.METHODS: Serum samples from sixty patients with chronic viral hepatitis B and twenty healthy blood donors were assayed to determine the level of PDGF-BB, TGF-β1, MMP-1, and TIMP-1 with ELISA, and HA, PCIII, C-IV, and LN level with RIA. The message RNA (mRNA) expression of TIMP-1 and MMP-1 in peripheral blood mononuclear cells (PBMCs) was detected by RT-PCR and Northern blot hybridization. Liver biopsy was performed in all patients.The biopsy samples were histopathologically examined. The trial was double-blind controlled.RESULTS: The serum level of PDGF-BB, TIMP-1, the ratio of TIMP-1 and MMP-1 (TIMP-1/MMP-1), mRNA expression of TIMP-1 (TIMP-1mRNA), and the ratio of TIMP-1mRNA and MMP-1mRNA (TIMP-1mRNA/MMP-1mRNA) in patients was significantly higher than those in the healthy blood donors (t=2.514-11.435, P=0.000-0.016). The serum level of PDGF-BB, TIMP-1, TIMP-1/MMP-1, and TIMP-1mRNA was positively correlated with fibrosis stage and inflammation grade (r=0.239-0.565, P=0.000-0.033), while the serum level of MMP-1 was negatively correlated with fibrosis stage and inflammation grade, and TIMP-1mRNA/MMP-1mRNA was positively correlated with inflammation grade. Through the analysis by ROC curve, serum PDGF-BB was the most valuable marker, and its sensitivity was the highest among the nine indices. The markers with the highest

  16. Effect of Four Weeks of β-alanine Supplementation on Muscle Carnosine and Blood Serum Lactate during Exercise in Male Rats.

    Science.gov (United States)

    Naderi, Alireza; Hemat Far, Ahmad; Willems, Mark E T; Sadeghi, Mehdi

    2016-01-01

    β-alanine (BA) supplementation may increase muscle buffering capacity and affect physiological responses during exercise. We examined the effects of 4 weeks of BA supplementation on muscle carnosine and serum lactate in male rats. Rats (n = 24, age: 2 months, body weight: 265±22 g) were divided into a BA supplementation or control group. Along with aerobic acclimatization exercise (15 m·min(-1), 8-10 min·day(-1), 4 days·week(-1) for 4 weeks), the BA group had access to BA powder in their drinking water (1.8%) with the control group having access to plain water for 4 weeks. After 4 weeks, rats ran on a treadmill at speeds of 15, 20, 25, 30, and 35 m·min(-1), respectively, each for 4 min, in order to measure post-exercise serum lactate. Muscle carnosine and serum lactate levels were measured with high-performance liquid chromatography (HPLC) and enzyme-linked immunosorbant assay (ELISA) procedures, respectively. Following BA supplementation, carnosine content in the m.rectus femoris increased by 117% (p carnosine content and reduces serum lactate; these changes may indicate an adaptation of rat skeletal muscles to postpone peripheral muscle fatigue during high-intensity exercise. PMID:26745664

  17. 急性缺血性脑血管病患者血清内皮素以及血糖、肌酐、尿素氮水平的研究%Study on levels of serum endothelin, blood glucose, creatinine and blood urea nitrogen in patients with acute ischemic cerebrovascular disease

    Institute of Scientific and Technical Information of China (English)

    赵华头; 汪杏; 徐旭然; 严婷婷

    2012-01-01

    目的 研究急性缺血性脑血管病患者血清内皮素以及血糖、肌酐、尿素氮水平变化规律,探讨神经功能缺损程度与血清内皮素水平之间的关系.方法 对照分析急性缺血性脑血管病患者与同期非神经系统疾病患者的血清内皮素以及血糖、肌酐、尿素氮水平.结果 急性脑梗死组患者血糖以及ET-1水平明显高于对照组,其他指标差异无统计学意义.血清ET-1水平与神经功能缺损程度具有显著的正相关性.结论防治胰岛素抵抗可以作为防治急性缺血性脑血管病的有效措施.血清ET-1水平可以作为急性缺血性脑血管病的敏感监测指标,对于预测患者预后具有重要意义.%Objective To study levels of the acute ischemic cerebrovascular disease in patients with serum endothelin and blood glucose,creatinine,blood urea nitrogen,and explore the relationship between the degree of neurological deficit and serum endothelin levels. Methods The serum endothelin and levels of blood sugar,creatinine,and blood urea nitrogen between patients with acute ischemic cerebrovascular disease and patients with non - nervous system disease were comparatively analyzed. Results The blood glucose and ET- 1 levels of patients with acute cerebral infarction were significantly higher than those of patients in the control group. The differences of the other indicators were not statistically significant. There was significant positive correlation between serum ET- 1 levels and the degree of neurological deficits. Conclusion The prevention and treatment of insulin resistance can be used as an effective measure to prevent and treat acute ischemic cerebrovascular disease. Serum ET- 1 levels can be used as sensitive indicators for monitoring of a-cute ischemic cerebrovascular disease,and of great significance for predicting the prognosis of patients.

  18. Analysis of variability of concentrations of valproic acid (VPA and its selected metabolites in the blood serum of patients treated with VPA and patients hospitalized because of VPA poisoning

    Directory of Open Access Journals (Sweden)

    Jolanta Wilimowska

    2015-03-01

    Full Text Available Aim of the study: To compare the metabolic profile of valproic acid (VPA in the studied groups of cases through an analysis of variability of concentrations of VPA with its selected metabolites (2-ene-VPA, 4-ene-VPA, 3-keto-VPA. Study material : Blood serum samples collected from 27 patients treated with VPA drugs in the Psychiatry Unit and in the Neurology and Cerebral Strokes Unit at the Ludwik Rydygier Provincial Specialist Hospital in Krakow, and blood serum samples collected from 26 patients hospitalized because of suspected acute VPA poisoning at the Toxicology Department, Chair of Toxicology and Environmental Diseases, Jagiellonian University Medical College in Krakow. Results and conclusions : The analysis of concentrations of VPA and its selected metabolites has shown that the metabolic profile of VPA determined in cases of acute poisoning is different from cases of VPA therapy. One of VPA’s metabolic pathways – the process of desaturation – is unchanged in acute poisoning and prevails over the process of -oxidation. The ingestion of toxic VPA doses results in an increased formation of 4-ene-VPA, proportional to an increase in VPA concentration. Acute VPA poisoning involves the saturation of VPA’s metabolic transformations at the stage of -oxidation. The process of oxidation of 2-ene-VPA to 3-keto-VPA is slowed down after the ingestion of toxic doses.

  19. Development and validation of an automated liquid-liquid extraction GC/MS method for the determination of THC, 11-OH-THC, and free THC-carboxylic acid (THC-COOH) from blood serum.

    Science.gov (United States)

    Purschke, Kirsten; Heinl, Sonja; Lerch, Oliver; Erdmann, Freidoon; Veit, Florian

    2016-06-01

    The analysis of Δ(9)-tetrahydrocannabinol (THC) and its metabolites 11-hydroxy-Δ(9)-tetrahydrocannabinol (11-OH-THC), and 11-nor-9-carboxy-Δ(9)-tetrahydrocannabinol (THC-COOH) from blood serum is a routine task in forensic toxicology laboratories. For examination of consumption habits, the concentration of the phase I metabolite THC-COOH is used. Recommendations for interpretation of analysis values in medical-psychological assessments (regranting of driver's licenses, Germany) include threshold values for the free, unconjugated THC-COOH. Using a fully automated two-step liquid-liquid extraction, THC, 11-OH-THC, and free, unconjugated THC-COOH were extracted from blood serum, silylated with N-methyl-N-(trimethylsilyl) trifluoroacetamide (MSTFA), and analyzed by GC/MS. The automation was carried out by an x-y-z sample robot equipped with modules for shaking, centrifugation, and solvent evaporation. This method was based on a previously developed manual sample preparation method. Validation guidelines of the Society of Toxicological and Forensic Chemistry (GTFCh) were fulfilled for both methods, at which the focus of this article is the automated one. Limits of detection and quantification for THC were 0.3 and 0.6 μg/L, for 11-OH-THC were 0.1 and 0.8 μg/L, and for THC-COOH were 0.3 and 1.1 μg/L, when extracting only 0.5 mL of blood serum. Therefore, the required limit of quantification for THC of 1 μg/L in driving under the influence of cannabis cases in Germany (and other countries) can be reached and the method can be employed in that context. Real and external control samples were analyzed, and a round robin test was passed successfully. To date, the method is employed in the Institute of Legal Medicine in Giessen, Germany, in daily routine. Automation helps in avoiding errors during sample preparation and reduces the workload of the laboratory personnel. Due to its flexibility, the analysis system can be employed for other liquid-liquid extractions as

  20. 星形细胞瘤预后与血清中基质金属白酶-2表达的关系%The relationship of expression of matrix metalloproteinase 2 in blood serum and prognostic of astrocytoma

    Institute of Scientific and Technical Information of China (English)

    向晖; 刘如恩

    2013-01-01

    目的 探讨基质金属蛋白酶-2(MMP-2)在血清中的表达与星形细胞瘤预后的关系.方法 随机选取46例经病理证实的星形细胞瘤患者,分为两组:A组为星形细胞瘤Ⅰ~Ⅱ级,B组为星形细胞瘤Ⅲ~Ⅳ级.分别对比两组患者术前,术后3、6、12个月的血清中MMP-2的含量,观察两组患者术前的差异以及复发前后之间的变化.结果 低级别星形细胞瘤术后血清中MMP-2水平为(9.7 ±3.1)g/L,高级别星形细胞瘤术后复发血清中MMP-2水平为(276.0 ±21.0)g/L,差异有统计学意义(P<0.01).结论 MMP-2在血清中的表达与星形细胞瘤术后复发密切相关.%Objective The aim of this article is to discuss the relationship of Expression of matrix metalloproteinase 2 (MMP-2) in blood serum and prognostic of astrocytoma.Methods 46 patients sufferd from astrocytoma are devided into 2 groups:group A contains patients of astrocytoma of grade Ⅰ-Ⅱ ; group B of grade Ⅲ-Ⅳ.The serum total level of MMP-2 are detected separately before surgery and in the 3rd,6th,12th month after surgery.Results Serum MMP-2 level in low grade Astrocytoma was (9.7 ± 3.1) g/L,serum MMP-2 level in high grade Astrocytoma Postoperative recurrence was (276.0 ± 21.0) g/L.The Expression was significant (P < 0.0l).Conclusion Expression of MMP-2 in blood serum is closely related to the grade and postoperative recurrence of astrocytoma.

  1. Effect of supplements: Probiotics and probiotic plus honey on blood cell counts and serum IgA in patients receiving pelvic radiotherapy

    OpenAIRE

    Hajar-Alsadat Mansouri-Tehrani; Mohammad Rabbani-Khorasgani; Sayyed Mohsen Hosseini; Fariborz Mokarian; Hoda Mahdavi; Mahnaz Roayaei

    2015-01-01

    Background: Radiotherapy is frequently used in treatment approaches of pelvic malignancies. Nevertheless, it has some known systemic effects on blood cells and the immune system that possibly results in their susceptibility to infection. Probiotics are live microbial food ingredients that provide a health advantage to the consumer. Honey has prebiotic properties. The aim of this clinical trial was to investigate probable effects of probiotic or probiotics plus honey on blood cell counts and s...

  2. THE CONTENT OF MICROELEMENTS IN BLOOD SERUM AND ERYTHROCYTES IN CHILDREN WITH DIABETES MELLITUS TYPE I DEPENDING ON LEVEL OF GLYCEMIC CONTROL.

    Science.gov (United States)

    Gluschenko, N; Vasylyshyn, Kh; Roschupkin, A; Lekishvili, S; Gladchenko, O

    2016-01-01

    The aim of this paper is to investigate the content of chromium, cobalt and nickel in serum and erythrocytes in children with type 1 diabetes mellitus, depending on the level of glycemic control. The study was conducted on 68 children with type 1 diabetes mellitus. The patients were divided into four groups based on glycemic control. Group I was composed of 9 children with optimal level of glycemic control. Group II - 25 children with suboptimal level of glycemic control. Group III - 34 children with a high risk to life level of glycemic control. Group IV (control group) consisted of 30 healthy children. Compensation state of type 1 diabetes was evaluated according to ISPAD (Consensus for the Management of Type 1 Diabetes Mellitus in Children and Adolescens 2000). The content of trace elements in biological agents was determined by atomic absorbtion spectrophotometry method with C-115M1 mass-spectrophotometer, manufactured by «Selmi» enterprise (Ukraine). It is found that there is a decrease in serum concentrations of chromium and erythrocyte content of cobalt in patients with optimal level of glycemic control. The deficiency of chromium is accompanied by the deficiency of cobalt in patients with suboptimal level of glycemic control. The lower levels of cobalt and nickel are recorded simultaneously, but there is theexcess of chromium in the erythrocytes of these patients. Patients, who suffer from 1 type diabetes mellitus and high risk for life level of glycemic control have considerable polideficiency of cobalt, nickel and chromium in serum.The increasing level of chromium was recorded only in the erythrocytes. The level of glycemic control and the duration of 1 type diabetes mellitus are important in the forecasting of the development of chronic diabetic complications. It is found that the duration of 1 type diabetes mellitus influences the levels of cobalt and nickel in serum mostly, while the level of glycemic control influences the chromium content. PMID

  3. Diagnostic informative value of gastroduodenal regulatory peptides of the blood serum on an empty stomach and after test breakfasts of various compositions

    International Nuclear Information System (INIS)

    Gastrin, secretin and cholecystokinin were determined by a radioimmunoassay in healthy persons (19) and in patients with peptic ulcer (13) on an empty stomach and after test breakfasts with different nutrients. In the healthy persons the blood concentration of regulatory peptides was lower than in the patients. Breakfasts increased the concentrations of gastrin, secretin and cholecystokinin in the patients much more than in the controls. Some differences in changes of the blood concentration of peptides were noted with regard to a type of test breakfast. However differentiated reactions of the endocrine apparatus of the gastroduodenal complex in response to the breakfasts were not a reliable functional and diagnostic criterion

  4. In vitro studies using Tc-99m-labelled human serum albumin millimicrospheres for assessing the contribution to phagocytosis of mononuclear blood cells

    International Nuclear Information System (INIS)

    Tc-99m-labelled HSA millimicrospheres were incubated with whole blood for various periods of time, in order to analyse the rate of fixation of HSA millimicrospheres to monocytes, and their interaction. It was not possible with the technique applied to differentiate between a genuine process of phagocytosis and a conceivable mere fixation of particles to the cells. (MBC)

  5. Effect of L- or DL-methionine Supplementation on Nitrogen Retention, Serum Amino Acid Concentrations and Blood Metabolites Profile in Starter Pigs.

    Science.gov (United States)

    Tian, Q Y; Zeng, Z K; Zhang, Y X; Long, S F; Piao, X S

    2016-05-01

    The objective of the current study was to evaluate the effect of supplementation of either L-methionine (L-Met) or DL-methionine (DL-Met) to diets of starter pigs on nitrogen (N) balance, metabolism, and serum amino acid profile. Eighteen crossbred (Duroc×Landrace×Yorkshire) barrows weighing 15.45±0.88 kg were randomly allotted to 1 of 3 diets with 6 pigs per treatment. The diets included a basal diet (Met-deficient diet) containing 0.24% standardized ileal digestibility Met with all other essential nutrients meeting the pig's requirements. The other two diets were produced by supplementing the basal diet with 0.12% DL-Met or L-Met. The experiment lasted for 18 days, consisting of a 13-day adaptation period to the diets followed by a 5-day experimental period. Pigs were fed ad libitum and free access to water throughout the experiment. Results showed that the supplementation of either L-Met or DL-Met improved N retention, and serum methionine concentration, and decreased N excretion compared with basal diet (p0.05). In conclusion, on equimolar basis DL-Met and L-Met are equally bioavailable as Met sources for starter pigs. PMID:26954214

  6. The bone mass density in men aged over 50 and its relation to the concentration of free and total testosterone in the blood serum

    International Nuclear Information System (INIS)

    As the mean length of life increases, osteoporosis affects a growing number of men and women, thus becoming an important medical and socioeconomic problem in many countries. Pathogenesis and the prevalence of the osteoporosis in women are well established, however, in men, they are still controversial. In this study, the bone mass density (BMD) of the lumbar spine was determined in 100 healthy men age 50-83, using quantitative computed tomography (QCT). Also, the total serum and free testosterone was measured. The mean BMD was 123.1I39.3 mg/cm3, and the values below a fracture threshold were noted in 39% of subjects. The mean concentration of total and free serum testosterone was 4.3I1.7 ng/ml and 6.2I3.7 pg/ml, respectively. There was a significant (p3, respectively). There was no correlation found between total testosterone and BMD. Results indicate that reduced bone mass density in males over 50 is as frequent as recently reported in females. Moreover, sex hormones seem to be related to osteoporosis development in men as well. (author)

  7. 小麦饲粮中添加木聚糖酶对肉鹅血糖和血清总蛋白水平的影响%Effect of Wheat Based Diet Supplemented with Xylanase on Blood Sugar and Total Protein in Serum of Geese

    Institute of Scientific and Technical Information of China (English)

    王佳丽; 史东辉; 杨桂芹

    2008-01-01

    [Objective] The effects of wheat based diet supplemented with xylarmse on blood sugar and total protein in serum of geese were studied. [Method] By using the randomized design of single factor, the 1-day-old healthy goslings were divided into 6 groups and fed with corn based diet, wheat based diet and wheat based diet supplemented with xylanase at different concentrations respectively, the contents of blood sugar and total protein in serum were determined. [Result] The wheat based diet supplemented with xylanase could increase the blood sugar and total protein in serum of geese and wheat based diet supplemented with 0.2% xylanase generated the best effect, which was higher than those of corn based diet group. As for the concentration of protein in senun, wheat based diet supplemented with O. 2% xylarmse was significantly different from corn based diet and wheat based diet. [Conclusion] The wheat based diet supplemented with xylanase could enhance geese production.

  8. 内源性n-3多不饱和脂肪酸对fat-1转基因小鼠血糖血脂的影响%Effects of Endogenous n-3 Polyunsaturated Fatty Acids (PUFAs) on Blood Glucose and Serum Lipid in fat-1 Transgenic Mouse Model

    Institute of Scientific and Technical Information of China (English)

    周飞; 张晓宏; 邹祖全; 张才乔

    2015-01-01

    利用fat-1转基因小鼠模型,研究内源性n-3多不饱和脂肪酸(n-3 PUFAs)的血糖血脂调节作用.采用fat-1转基因小鼠和C57BL/6野生型小鼠喂食高n-6、低n-3 PUFAs的标准配方饲料4周,然后2组小鼠给予高糖饮液自由饮用4周,每周测体重.第8周末,取血离心测血糖、胰岛素、甘油三脂(TG)、胆固醇(TC)、高密度脂蛋白(HDL-C)、低密度脂蛋白(LDL-C)水平.结果表明:fat-1转基因小鼠体重增加幅度、空腹血糖值、血清胰岛素、胰岛素抵抗指数、血清TC、TG、HDL-C、LDL-C水平明显低于野生型小鼠(P<0.05).认为n-3 PUFAs能抑制体重增长,降低小鼠血糖、血脂和胰岛素抵抗,起到调节血糖、血脂的作用.%In this study, we investigate the effects of endogenous n-3 PUFAs on blood glucose and serum lipid in fat-1 transgenic mouse model. The transgenic and wild type mice are maintained on a 10%corn oil diet which has high ratio of n-6/n-3 polyunsaturated fatty acids for 4 weeks. Then make them drink high sugar pinocytosis freely for another four weeks. On the eighth weekend, serum glucose, insulin, serum triglyceride (TG), serum total cholesterol (TC), serum high-density lipoprotein cholesterol (HDL-C), serum low-density lipoprotein cholesterol (LDL-C) levels of blood serum are tested after blood collection and centrifugation. The weight gain rate, fasting blood sugar and serum insulin levels, insulin resistance index, serum TC, TG, HDL-C, LDL-C of fat-1 transgenic mice are found to be significantly lower than those of wild type mice (P<0.05). The tests indicate that n-3 polyunsaturated fatty acids can reduce the blood glucose and serum lipid, which may play a vital role in regulating blood lipid and decreasing insulin resistance.

  9. Kolaviron Improved Resistance to Oxidative Stress and Inflammation in the Blood (Erythrocyte, Serum, and Plasma) of Streptozotocin-Induced Diabetic Rats

    OpenAIRE

    Ayepola, Omolola R.; Brooks, Nicole L.; Oguntibeju, Oluwafemi O

    2014-01-01

    Aims. Bitter kola seed (Garcinia kola, family: Guttiferae) has been used as a social masticatory agent in Africa for several years and is believed to possess many useful medicinal properties. The present study evaluates the antioxidative, anti-inflammatory, and antilipidemic effects of kolaviron (an extract from the Garcinia kola seeds) in the blood of streptozotocin- (STZ) induced diabetic rats. Methods. Diabetic rats were treated with kolaviron (100 mg/kg b·wt) orally, five times a week for...

  10. Assessing the sensitivity and specificity of First Response HIV-1-2 test kit with whole blood and serum samples: a cross-sectional study

    OpenAIRE

    Boadu, Raymond; Darko, George; Nortey, Priscilla; Akweongo, Patricia; Sarfo, Bismark

    2016-01-01

    Background Human immunodeficiency virus (HIV) Rapid diagnostic Test (RDT) kits are the preferred assays for HIV testing in many countries. Prevention of Mother-to-Child Transmission, Know Your Status Campaigns, Blood-Safety, Voluntary Counseling and Testing are major strategies adapted to control transmission of the virus and the pivot of these interventions is either screening or diagnosing individuals through testing. There are reports of inconsistent sensitivity and specificity with whole ...

  11. Direct analysis of blood serum by total reflection X-ray fluorescence spectrometry and application of an artificial neural network approach for cancer diagnosis*1

    Science.gov (United States)

    Hernández-Caraballo, Edwin A.; Marcó-Parra, Lué M.

    2003-12-01

    Iron, copper, zinc and selenium were determined directly in serum samples from healthy individuals ( n=33) and cancer patients ( n=27) by total reflection X-ray fluorescence spectrometry using the Compton peak as internal standard [L.M. Marcó P. et al., Spectrochim. Acta Part B 54 (1999) 1469-1480]. The standardized concentrations of these elements were used as input data for two-layer artificial neural networks trained with the generalized delta rule in order to classify such individuals according to their health status. Various artificial neural networks, comprising a linear function in the input layer, a hyperbolic tangent function in the hidden layer and a sigmoid function in the output layer, were evaluated for such a purpose. Of the networks studied, the (4:4:1) gave the highest estimation (98%) and prediction rates (94%). The latter demonstrates the potential of the total reflection X-ray fluorescence spectrometry/artificial neural network approach in clinical chemistry.

  12. Direct analysis of blood serum by total reflection X-ray fluorescence spectrometry and application of an artificial neural network approach for cancer diagnosis

    International Nuclear Information System (INIS)

    Iron, copper, zinc and selenium were determined directly in serum samples from healthy individuals (n=33) and cancer patients (n=27) by total reflection X-ray fluorescence spectrometry using the Compton peak as internal standard [L.M. Marco P. et al., Spectrochim. Acta Part B 54 (1999) 1469-1480]. The standardized concentrations of these elements were used as input data for two-layer artificial neural networks trained with the generalized delta rule in order to classify such individuals according to their health status. Various artificial neural networks, comprising a linear function in the input layer, a hyperbolic tangent function in the hidden layer and a sigmoid function in the output layer, were evaluated for such a purpose. Of the networks studied, the (4:4:1) gave the highest estimation (98%) and prediction rates (94%). The latter demonstrates the potential of the total reflection X-ray fluorescence spectrometry/artificial neural network approach in clinical chemistry

  13. [Analysis of the connection between the level of protein fractions and immunoglobulins in blood serum with irradiation in nuclear power enterprise workers].

    Science.gov (United States)

    Tel'nov, V I

    1996-01-01

    989 workers of atomic industry at the age from 35 to 78 years old subjected to the general external gamma radiation and incorporation of plutonium 239 in a wide range of doses about 17-40 years ago have been examined for the protein indices with the radiation effect bearing in mind nine non-radiation factors. The step-by-step regression analysis revealed a positive linear link for the total protein level, absolute content of alpha-I-globulins with the total dose of external gamma radiation. Increasing of serum proteins entropy, correlated with external radiation by protein shifts effect, had not coincided with its age increasing. The influence of plutonium-239 incorporation on the examined protein parameters was not found. PMID:9254521

  14. Influence of a chinese traditional medicine recipes for replenishing kidney function and activating blood circulation on levels of serum with interleukin-6 and other hormones affecting calcium metabolism in female rats after ovariectomy

    International Nuclear Information System (INIS)

    Objective: To investigate the influence of a Chinese traditional medicine recipe for replenishing kidney function and activating blood circulation (CRRK) in female rats on serum levels of interleukin-6 (IL-6) and other hormones affecting calcium metabolism after ovariectomy. Methods: Ninety female SD rats were divided into 6 groups: control, model (ovariectomy only) treated with nyl-estriol, treated with CRRK (high, moderate and low doses). CRRK (Gu Kang) was administered at the three dose levels for 90 days. Radioimmunoassay was used to determine the serum levels of estradiol (E2), IL-6, calcitonin (CT) and BGP. The weight and histo-morphology of the uterus of the rats sacrificed after 90 days of treatment was also studied. Results: Compared with the control group, the levels of IL-6 in the model group were significantly higher (P 2 and CT, BGP were significantly lower (P 2 and BGP were significantly higher (P < 0.05-0.001). The weight of uterus was also increased (P < 0.05). The composite recipe proved to have definite effects on normalizing the uterine histomorphology. Conclusion: The results had showed that the composite recipe had good effects on prevention and therapy of osteoporosis after ovariectomy in rats

  15. 波纹唇鱼血细胞显微结构和血液生化指标%Microstructure of Peripheral Blood Cell and Serum Biochemical Indices of Cheilinus undulatus

    Institute of Scientific and Technical Information of China (English)

    廖光勇; 区又君; 李加儿

    2011-01-01

    以Wright's和Giemsa双重染色法对波纹唇鱼(Cheilinus undulatus)外周血细胞的形态进行显微观察研究并计数,同时测定各类血细胞的大小及血液的生化指标.血涂片观察发现:波纹唇鱼的外周血细胞可区分出红细胞、嗜中性粒细胞、单核细胞、淋巴细胞、血栓细胞等五种血细胞,未观察到嗜碱性粒细胞、嗜酸性粒细胞.在外周血液中还观察到核影、正在分裂的红细胞、幼稚红细胞和降解的红细胞.各类血细胞胞体由大到小的次序依次为单核细胞、嗜中性粒细胞、红细胞、大淋巴细胞、血栓细胞、小淋巴细胞.血浆中红细胞为(3.15±0.79)×1012/L,白细胞为(1.42±0.71)×1010/L,红细胞数远远多于白细胞.血液生化指标与其他鱼类相比有一定差异,表明鱼类血液的生化成分因种和生活环境而异.%The microscopic observation and count are conducted for the peripheral blood cells of C. Undula-tus by means of the Wright's and Giemsa double dye method. The serum biochemical indices of C. Undula-tus , the sizes of the various blood cells are determined. From the stained smears, five blood cell types I. E. Erythrocyte, neutrophil, monocyte, lymphocyte and thrombocyte are detected. Neither eosinophilic granu-locyten nor basophilic granulocyte is found but, some nuclear shadow of erythrocytes, immature erythro-cytes, direct dividing or dissolving erythrocytes. Among these cells, the sizes are put into an order from large to small as follows monocyte, neutrophil, erythrocyte, large lymphocyte, thrombocyte and small lymphlcyte. The erythrocyte and leucocyte concentrations in the blood are (3. 15 ±0. 79) × 1012/L and (1. 42 + 0. 71) × 1010/L respectively. The number of erythrocyte is much more than the leucocyte in the blood. The serum biochemical indices are different from other fish species. Therefore it is indicated that the serum biochemical indices for various fishes depend upon their species and

  16. Detection of benzo[a]pyrene diol epoxide-DNA adducts in peripheral blood lymphocytes and antibodies to the adducts in serum from coke oven workers.

    OpenAIRE

    Harris, C. C.; Vahakangas, K.; Newman, M J; Trivers, G E; Shamsuddin, A; Sinopoli, N; Mann, D L; Wright, W. E.

    1985-01-01

    Coke oven workers are exposed to high levels of carcinogenic polycyclic aromatic hydrocarbons, including benzo[a]pyrene (B[a]P), and are at increased risk of lung cancer. Since B[a]P is enzymatically activated to 7 beta,8 alpha-dihydroxy(9 alpha, 10 alpha)epoxy-7,8,9,10-tetrahydrobenzo[a]pyrene (B[a]PDE) that forms adducts with DNA, the presence of these adducts was measured in DNA from peripheral blood lymphocytes by synchronous fluorescence spectrophotometry and enzyme radioimmunoassay. App...

  17. Alterations in the blood glucose, serum lipids and renal oxidative stress in diabetic rats by supplementation of onion (Allium cepa. Linn)

    OpenAIRE

    Bang, Mi-Ae; Kim, Hyeon-A; Cho, Young-Ja

    2009-01-01

    This study examined the anti-diabetic effect of onion (Allium cepa. Linn) in the streptozotocin (STZ)-induced diabetic rats. Male Sprague-Dawley rats were divided into normal rats fed control diet or supplemented with onion powder (7% w/w) and diabetic rats fed control diet or supplemented with onion powder. Diabetes was induced by a single injection of STZ (60 mg/kg, ip) in citrate buffer. The animals were fed each of the experimental diet for 5 weeks. Blood glucose levels of rats supplement...

  18. Lipid profiles of blood serum and fatty acid composition of meat of hybrid duck fed diet supplemented with Noni (Morinda citrifolia fruit meal

    Directory of Open Access Journals (Sweden)

    David Kurniawan

    2015-09-01

    Full Text Available Noni fruit is a medicinal plant with biological activity like antioxidant that could potentially be used as a feed additive in poultry. This research investigated the effect of noni fruit powder as feed additive on lipid profiles of blood and meat fatty acid compositions of meat of hybrid duck. One hundred twenty 2-week-old hybrid ducks crossing between Peking and Khaki Campbell duck were subjected. They were randomly allotted to 24 experimental units. Each experimental unit was 70x80x40 cm in size and it was used for 5 ducks up to they reached 56 days of age. Each unit was equipped with waterer and feeder. The ducks were raised on litter-type floor. The basal experimental diet was formulated according to the standards of National Research Council (1994. The method used for this study was experimental with 4 different treatments in 6 replications. The treatments were as follow: P0: basal feed without supplementation of noni fruit powder as control; P1: basal feed + 1 % noni fruit powder; P2: basal feed + 2 % noni fruit powder; P3: basal feed + 3 % noni fruit powder. Data were analyzed by one-way of Completely Randomized Design ANOVA and if there was significant effect followed by Duncan’s Multiple Range Test. Result showed that using noni fruit powder as feed additive had no significant effect (P>0.05 on lipid profiles of blood and fatty acid composition of meat.

  19. Serum Ferritin in Thalassemia Intermedia

    OpenAIRE

    Shah, Ravi; Trehan, Amita; Das, Reena; R K Marwaha

    2013-01-01

    Serum ferritin is a useful monitoring tool for iron overload in thalassemia major. In resource poor settings access to modalities for assessment of iron overload are limited. This study was undertaken to assess the efficiency and usefulness of serum ferritin level in thalassemia intermedia (TI) patients. This was a cross sectional observational study. Seventy seven TI patients attending the pediatric hematology clinic were included. Fasting blood sample was taken from each patient in iron fre...

  20. Colorimetric and fluorescence quenching aptasensors for detection of streptomycin in blood serum and milk based on double-stranded DNA and gold nanoparticles.

    Science.gov (United States)

    Emrani, Ahmad Sarreshtehdar; Danesh, Noor Mohammad; Lavaee, Parirokh; Ramezani, Mohammad; Abnous, Khalil; Taghdisi, Seyed Mohammad

    2016-01-01

    Antibiotic residues in animal foodstuffs are of great concern to consumers. In this study, fluorescence quenching and colorimetric aptasensors were designed for detection of streptomycin based on aqueous gold nanoparticles (AuNPs) and double-stranded DNA (dsDNA). In the absence of streptomycin, aptamer/FAM-labeled complementary strand dsDNA is stable, resulting in the aggregation of AuNPs by salt and an obvious color change from red to blue and strong emission of fluorescence. In the presence of streptomycin, aptamer binds to its target and FAM-labeled complementary strand adsorbs on the surface of AuNPs. So the well-dispersed AuNPs remain stable against salt-induced aggregation with a wine-red color and the fluorescence of FAM-labeled complimentary strand is efficiently quenched by AuNPs. The colorimetric and fluorescence quenching aptasensors showed excellent selectivity toward streptomycin with limit of detections as low as 73.1 and 47.6 nM, respectively. The presented aptasensors were successfully used to detect streptomycin in milk and serum. PMID:26212949

  1. Gamma-glutamyl transferase activity in fetal serum, maternal serum, and amniotic fluid during gestation.

    OpenAIRE

    Moniz, C; Nicolaides, K H; Keys, D.; Rodeck, C H

    1984-01-01

    Gamma-glutamyl transferase activity was measured in fetal serum, maternal serum, and amniotic fluid in 173 pregnancies from 15 to 40 weeks' gestation. Fetal serum was obtained in the second trimester by fetoscopy and in the third trimester by umbilical cord puncture at caesarian section or vaginal delivery. Enzyme activities in maternal blood (10 IU/1, SD 2) and fetal blood (88 IU/1, SD 20) remained relatively constant throughout gestation, whereas in the amniotic fluid there was a significan...

  2. Comparative Analysis of Serum Renal Function, Blood Glucose and Amylase in Different Detection Systems%不同检测系统测定血清肾功、血糖、淀粉酶结果对比分析

    Institute of Scientific and Technical Information of China (English)

    徐新生

    2015-01-01

    目的:探讨不同检测系统测定血清肾功、血糖、淀粉酶结果对比;方法将我院检验科收入的300例患者的血清样品分别采用两种不同的检测系统进行检验,比较两种检测系统血清肾功、血糖及淀粉酶水平;结果不同检测系统测定的肾功、血糖及淀粉酶结果无差异,比较无统计学意义(>0.05);不同检测系统在肌酐、血糖及淀粉酶测定的精密度无差异;结论两种检测系统对肾功、血糖及淀粉酶测定结果具有可比性,测定的精密度较高,能够满足临床使用要求。%Objective To compare the detection results of renal function,blood sugar and amylase based on dif erent systems.Methods 300 serum samples col ected from our Clinical Laboratory were tested by two systems.The levels of renal function,blood sugar and amylase were compared.Results Based on the dif erent detection systems,the levels of renal function,blood sugar and amylase were not dif erent ( <0.05);based on the dif erent systems,the detection precision of Creatinine,blood sugar and amylase was not dif erent.Conclusion The detection results of renal function,blood sugar and amylase based on two systems are comparable and have a higher detection precision,which it conforms to the clinical requirements.

  3. Effects of Dietary Furundu (Fermented Hibiscus sabdariffa Seed Supplementation on the Performance, Some Blood and Serum Parameters and Histopathology of Wistar Rats

    Directory of Open Access Journals (Sweden)

    Warda S. Abdelgadir

    2012-04-01

    Full Text Available The present study was conducted to obtain information on the effects of various dietary doses of the fermented Hibiscus sabdariffa seed (Furundu on Wistar rats. Emphasis was put on changes on growth, lesions, alteration in hematology and serobiochemical constituents and histopathology of treated rats. Fermented Hibiscus sabdariffa seed product (Furundu was fed to Wistar rats at 10, 15 and 20%, respectively of the standard diet for 12 weeks. Incorporation in the diet of the three doses of both was toxic but not fatal. Depression in growth and hepatonephrotoxicity were observed in rats that had been given 10, 15 and 20% Furundu, respectively for 12 weeks. These findings were accompanied by leukocytosis, macrocytic normochromic anaemia in rats fed on 20% Furundu and macrocytic hypochromic, normocytic hypochromic anaemia in those fed on 10 and 15% Furundu respectively, in addition to alterations in serum aspartate aminotransferase (AST, alanine aminotransferase (ALT and alkaline phosphatase (ALP activity and albumin, globulin, bilirubin and cholesterol concentrations. At the end of the experimental period (12 weeks, the total protein of all the test groups and the globulin concentration of group 2 fed on 15% Furundu decreased significantly (p<0.05, albumin concentration of all the test groups and the urea concentration of group 3 fed on 15% Furundu remained unchanged. Histopathological examination showed Fatty cytoplasmic vacuolation of the centrilobular hepatocytes and lymphocytic infiltration, desquamation of the intestinal epithelium and intestinal congestion were noticed in rats fed on 20% Furundu. Fermented Hibiscus sabdariffa seed product Furundu is considered enterohepatonephrotoxic to Wistar rats at dietary levels of 10, 15 and 20%, respectively.

  4. Alterations of serum concentrations of thyroid hormones and sex hormone-binding globulin, nuclear binding of tri-iodothyronine and thyroid hormone-stimulated cellular uptake of oxygen and glucose in mononuclear blood cells from patients with non-thyroidal illness

    DEFF Research Database (Denmark)

    Kvetny, J; Matzen, L

    1990-01-01

    Nuclear tri-iodothyronine (T3) binding and thyroid hormone-stimulated oxygen consumption and glucose uptake were examined in mononuclear blood cells from patients with non-thyroidal illness (NTI) in which serum T3 was significantly (P less than 0.05) depressed (0.62 +/- 0.12 (S.D.) nmol/l) compar...

  5. Serum progesterone

    Science.gov (United States)

    ... make a woman's uterus ready for a fertilized egg to be implanted. It also prepares the uterus for pregnancy and the breasts for milk production. How the Test is Performed A blood sample is needed. Most of the time blood is drawn from a vein located on the inside of the elbow or the back of the ...

  6. Influence of Burn Infection on Complement C3 and IL-10 Level in Blood Serum%烧伤感染对血清中补体 C3和 IL-10水平的影响

    Institute of Scientific and Technical Information of China (English)

    闫沛; 李武平; 代艳然

    2012-01-01

      目的分析烧伤感染对血清中补体 C3和 IL-10水平的影响,旨在根据其变化水平及时给予合适的免疫调节治疗,预防感染的发生.方法分别采集患者烧伤后第3、7、14天的创面分泌物和外周血,对分泌物做细菌培养,用酶联免疫吸附测定法(Enzyme-linked immunosorbent assay, ELISA)检测血清中的补体 C3和 IL-10水平,病情严重疑有脓毒血症者及时进行血培养.结果烧伤后创面感染率不断上升,脓毒血症发生率达16.4%,对补体 C3水平的影响无统计学意义(P >0.05),但烧伤后第14天脓毒血症患者血清中 IL-10水平是创面感染患者的2倍,相比有统计学意义(P 0. 05). However, at the four-teenth day postburn, the level of IL-10 in blood serum of patients with septicopyemia was two times over that of the patients with burn infection, and the comparison had significance in statistics (P <0. 05). Conclusion The increase of complement C3 level can improve the body immunity but can not reflect the infection situation of the body, while the excessive increase of IL-10 level signifies the aggravation of infection to some extent, and in clinic, the auxiliary detection of IL-10 level in blood serum can help to discover the tendency of infection aggravation and so that prompt immunoregulation can be given.

  7. Significance of serum procalcitonin and blood gas analysis in the risk anticipation of patients with acute exacerbation of COPD%血清降钙素原联合血气分析检测对AECOPD患者临床风险预测的意义

    Institute of Scientific and Technical Information of China (English)

    曹友红

    2013-01-01

    Objective To estimate the prognostic value of serum procalcitonin and blood gas analysis in the risk anticipation of patients with acute exacerbation of chronic obstructive pulmonary disease ( AECOPD ). Methods The study selected 65 patients with AECOPD as the test group and 50 healthy people as the control group. Their PCT level and arterial blood gas analysis indexes were tested and dynamically monitored 5 days after the treatment. Results Serum procalcitonin and blood gas analysis had a high sensitivity, and the differences showed a statistical significance before and after the treatment (P 0.05).结论 联合检测血清降钙素原及血气分析对AECOPD患者的风险预测具有较重要的临床意义.

  8. Serum Adiponectin in Women with Gestational Diabetes

    OpenAIRE

    2014-01-01

    Background &Objective: Adiponectin is an adipose tissue adipokin that may contribute to obesity and insulin resistantance. The aim of this study was to evaluate the associations between serum concentrations of adiponectin and insulin resistance in gestational diabetes (GDM).Materials & Methods: Serum adiponectin levels, fasting blood sugar (FBS), glycated hemoglobin (HbA1C), insulin levels and blood lipids were measured in 66 women with GDM and 70 pregnant women without GDM. The associations ...

  9. 成人身体成分与血脂、血糖关系的探讨%Analysis of the relationship between body composition and fasting serum lipids, fasting blood glucose in adult

    Institute of Scientific and Technical Information of China (English)

    李秋桂; 姬芳勤

    2014-01-01

    目的:分析成人身体成分与血脂、血糖指标之间的关系方法选择成年体检者490名,排除严重心脑肝肾疾病,肿瘤患者,急性炎症患者及长期服药者等。通过Biospace InBody 220人体成分分析仪对身体水分、蛋白质、无机盐、体脂肪、体重、骨骼肌肉量、体质指数、体脂百分比、腰臀脂肪比等指标进行测定。同时测定空腹血糖、血脂指标。所有指标的测定均由我院医护人员经过统一培训后进行操作。结果男女性别年龄无差异(p>0.05)。除体脂肪外,男女性别间身体水分、蛋白质、无机盐、体重、骨骼肌肉量、体质指数、体脂百分比、腰臀脂肪比均有显著差异(均p<0.001)。蛋白质、体脂肪、体重、骨骼肌肉量、体质指数、体脂百分比、腰臀脂肪比与空腹血糖呈正相关(均p<0.05)。身体水分、蛋白质、无机盐、体脂肪、体重、骨骼肌肉量、体质指数、体脂百分比、腰臀脂肪比均可对血脂(总胆固醇、甘油三酯)产生影响(均p<0.05)。剔除性别因素后,对身体成分与血糖、血脂各指标进行偏相关分析,体脂肪、体重、体质指数、体脂百分比、腰臀脂肪比仍然与血糖、总胆固醇、甘油三酯呈正相关(均p<0.05)。结论成人身体成分与血糖、血脂指标密切相关。通过身体成分的分析,可尽早预防代谢相关性疾病。%Objective To analyze the relationship between body composition and fasting serum lipids, fasting blood glucose in adult. Methods Select 490 adult, eliminate the subjects who has the serious cardiovascular diseases, liver diseases , kidney diseases, cancer and the subjects with acute inflammation and long term medication etc. Body moisture, protein, inorganic salt, fat, skeletal muscle weight, body mass index, percentage of body fat, waist and hip fat ratio was determined by Biospace InBody 220. Measure the

  10. Ready-made allogeneic ABO-specific serum eye drops

    DEFF Research Database (Denmark)

    Harritshøj, Lene Holm; Nielsen, Connie; Ullum, Henrik;

    2014-01-01

    severe ocular surface disorders. METHODS: Serum was derived from 450 ml whole-blood donations from regular male blood donors, produced and tested according to good manufacturing practice and legislation regulating blood products in Denmark. Serum was diluted to 20% (v/v) with NaCl 0.9%, filtered, bottled......PURPOSE: To overcome problems and delays of the preparation of autologous serum eye drops, a production line of ABO-specific allogeneic serum eye drops from male blood donors was set up in a blood bank. Feasibility, clinical routine, safety and efficacy were evaluated in a cohort of patients with......, registered and stored at -30°C in the blood bank. Upon request, frozen ABO-identical serum drops in lots of 14 bottles could be provided immediately. Safety and efficacy were evaluated in 34 patients with severe ocular surface disease refractory to conventional medical therapy. Patients were treated six...

  11. Blood Clots

    Science.gov (United States)

    ... Index A-Z Blood Clots Blood clots are semi-solid masses of blood that can be stationary (thrombosis) ... treated? What are blood clots? Blood clots are semi-solid masses of blood. Normally, blood flows freely through ...

  12. Value of ABO blood group antibody titers in serum of pregnant woman of O-type blood in late pregnancy in predicting neonatal hemolytic disease%O型血孕妇妊娠晚期血清ABO血型抗体滴度对新生儿溶血病的预测价值

    Institute of Scientific and Technical Information of China (English)

    冯丽瑾

    2016-01-01

    Objective To analyze the value of ABO blood group antibody antibody titers in serum of pregnant woman of O-type blood in late pregnancy in predicting neonatal hemolytic disease.Methods Totally 655 pregnant women of O-type blood in late pregnancy from January 2014 to April 2015 underwent detection of ABO blood group antibody antibody titers in serum of pregnant woman;according to the IgG anti-A (B) were divided into5 groups (≤64,128 and >64,≤256 and >128,≤512 and >256,>512).The three standard tests of hemolytic disease was performed in neonates with neonatal hemolytic disease.The correlation of neonatal hemolytic disease and ABO blood group antibody antibody titers in serum of pregnant woman in late pregnancy was analyzed in 655 neonates.Results There were 103 (15.7%) strongly positive patients in neonatal antibody elution test.There were respectively 5 cases (1.6%,5/307),15 cases (12.6%,15/119),19 cases (26.4%,19/72),28 cases (38.9%,28/72),36 cases (42.4%,36/85) of neonatal hemolytic disease in pregnant women with antibody antibody titers ≤64,≤128 and >64,≤256 and >128,≤512 and >256,>512,with significant differences between groups (all P < 0.05).The levels of total and indirect bilirubin in neonatal serum decreased with increasing of antibody antibody titer (P < 0.05).Conclusion In pregnant women in late pregnancy,the ABO blood group antibody antibody titer in serum has the clinal significance of monitoring the occurence of neonatal hemolytic disease.%目的 分析0型血孕妇妊娠晚期血清ABO血型抗体滴度对新生儿溶血病的预测价值.方法 选取从2014年1月1日到2015年4月30日期间于江苏省苏州市立医院进行产前检测的O型血孕妇655例作为研究对象,检测其ABO血型抗体滴度,按IgG抗-A(B)滴度分为5组(≤64,≤128且>64,≤256且>128,≤512且>256,>512),并对发生新生儿溶血病的患儿进行新生儿溶血3项试验检查,观察655名新生儿中新

  13. Association between dietary habits, education, serum triglycerides and blood cholesterol among women of Cabildo, Buenos Aires Asociación entre hábitos nutricionales, educación, triglicéridos séricos y colesterol total en mujeres de Cabildo, Buenos Aires

    OpenAIRE

    Raúl J. Schneider; Noel Barengo; Irja Haapala; Marcelo Tavella

    2006-01-01

    A cross sectional study of 107 women between 20 and 69 years old, living in the town of Cabildo, province of Buenos Aires, Argentina, which describes food intake and analyses its relation to their education, blood cholesterol and serum triglyceride levels. A food frequency questionnaire including questions regarding meal patterns and food use were completed by the participants. Questions regarding educational status were included. A nutritional risk score was created from nine food groups. To...

  14. Serum ferritin

    International Nuclear Information System (INIS)

    Serum ferritin (SF) concentration as determined by the immunoradiometric method allows the direct measurement of a fraction of the body ferritin pool. In normal subjects, SF is an excellent index of body iron stores. In certain conditions associated with increased ferritin synthesis (such as liver disease, inflammation, malignancy, chronic disorders, ineffective erythropoiesis, or during ferrotherapy), SF may not accurately reflect body iron stores. In hyposideremic anemias SF concentration permits to differentiate those due to iron deficiency from those due to chronic disorders. With a good assay quality, subnormal SF levels are incontrovertible in the diagnosis of iron deficiency. SF determination has been investigated as possible tumor marker. When performed in combination with the alpha-fetoprotein assay, SF enhances the specificity of serodiagnosis of hepatoma. SF results must be interpreted bearing in mind the possible participation of circumstances that i) modify the body iron stores and ii) lead to increased ferritin synthesis. (author)

  15. Serum pancreatic lipase as a screening test for cystic fibrosis.

    OpenAIRE

    Adriaenssens, K; Van Riel, L

    1982-01-01

    Pancreatic lipase catalyses the hydrolysis of emulsified triglycerides to form a transparent solution of monoglycerides and fatty acids. Levels of serum pancreatic lipase were measured in neonates known to have cystic fibrosis and compared with levels in control infants. During the first weeks of life infants with cystic fibrosis had raised serum pancreatic lipase values in parallel with raised serum trypsin values. A simple and specific turbidimetric dried blood spot assay for serum pancreat...

  16. Correlation of serum leptin with levels of hemoglobin in hemodialysis

    Directory of Open Access Journals (Sweden)

    Rafieian-Kopaei Mahmoud

    2012-04-01

    Full Text Available To examine the association of serum leptin level with anemia in hemodialysis, we investigated 36 patients (males: 21, diabetics: 11 under regular hemodialysis. For patients, complete blood counts, iron profile, serum leptin, and adequacy of hemodialysis were assessed. In this study a significant correlation of serum leptin with level of hemoglobin and body mass index was detected. An association between serum leptin and total iron binding capacity was observed. No correlation of serum ferritin with leptin level was seen. Our findings attest previous findings showing that greater serum leptin levels are associated with greater hemoglobin levels.

  17. CO2 blood test

    Science.gov (United States)

    Bicarbonate test; HCO3-; Carbon dioxide test; TCO2; Total CO2; CO2 test - serum ... Many medicines can interfere with blood test results. Your health care provider will tell you if you need to stop taking any medicines before you have this test. DO ...

  18. 血管内皮生长因子在妊娠期糖尿病产妇所分娩新生儿脐血中的表达%Expression of vascular endothelial growth factor in serum of umbilical blood from the newborns of women with gestational diabetes mellitus

    Institute of Scientific and Technical Information of China (English)

    王萍; 张巍; 陈素娟

    2009-01-01

    Objective It is to study the expression and significance of vascular endothelial growth factor(VEGF) in serum of umbilical blood from the newborns of women with gestational diabetes mellitus(GDM). Methods Radioimmunoassay(RIA) was used to measure serum VEGF level of umbilical blood from 31 newborns of women with GDM(infants of diabetes mothers , IDM group) and 30 newborns of normal pregnancy women(control group). According to blood sugar, IDM group included 18 newborns with normal blood sugar(normal blood sugar group) and 13 newborns with hypoglycemia (hypoglycemia group). The level of blood sugar of 30 cases in control group was normal. Results The VEGF level of umbilical vein blood and umbilical artery blood in IDM group was significantly higher than that in control group (P<0.01). In control group, the VEGF level in umbilical artery blood was significantly higher than that in umbilical vein blood(P<0.05). VEGF level of umbilical artery blood in IDM hypoglycemia group was significantly higher than that in control group as well as in IDM normal blood sugar group(P<0.01 and P<0.05). Conclusion The serum VEGF level of umbilical vein and artery blood from the IDM was significantly higher than that of normal pregnancy women. The VEGF level of umbilical artery blood can reflect the severity degree of damage of GDM to their newborns.%目的 探讨血管内皮生长因子(VEGF)在妊娠期糖尿病(GDM)产妇分娩的新生儿(IDM)脐静脉、脐动脉血中的表达及其意义.方法 采用放射免疫分析法检测31例妊娠期糖尿病患者分娩的新生儿(IDM组)与30例健康产妇所分娩的新生儿(对照组)脐静脉、脐动脉血VEGF水平,IDM组新生儿按其出生后血糖水平分为IDM血糖正常组(18例)和IDM低血糖组(13例),对照组30例新生儿血糖均正常.结果 IDM组脐静脉血和脐动脉血VEGF水平均显著高于对照组(P均<0.01).对照组脐动脉血VEGF水平显著高于脐静脉血水平(P<0.05).IDM低血糖

  19. HPLC Analysis of Fresh Rehmannia Glutinosa Libosch in Serum of Rats with Blood Heat and Hemorrhage Syndrome Animal Model%血热出血模型大鼠血清中鲜地黄高效液相色谱分析

    Institute of Scientific and Technical Information of China (English)

    郜新莲; 段红福; 崔瑛; 冯静; 杨云

    2011-01-01

    目的:对阴虚血热出血大鼠含鲜地黄血清进行分析,探索鲜地黄清热凉血药效的物质基础.方法:给予血热出血症模型大鼠鲜地黄,采集血清,用高效液相色谱法分析大鼠含药血清、正常血清以及鲜地黄药材的色谱,研究鲜地黄在血中移行成分以及鲜地黄对大鼠血清中固有成分的影响.结果:在血热出血症模型大鼠含药血清中检测到了鲜地黄的有效成分——梓醇的色谱峰;且发现血清中和药材中有3种相同的共有成分;鲜地黄对模型大鼠血清中1种固有成分的升高有抑制作用.结论:鲜地黄中梓醇及3种未明成分可能是其清热凉血物质基础;鲜地黄对动物血清中固有成分代谢的影响可能是鲜地黄清热凉血药效的机制.%Objective:To investigate bioactive components of fresh Rehmannia glutinosa Libosch. In heat - clearing and blood - cooling efficacy by analysis of it - contained serum of rats with yin - deficiency and heat - flourishing hemorrhage. Methods; High - performance liquid cinematography ( HPLC) was adopted to analyze the differences of chromatographs among drug - contained serum, the normal serum and fresh R. Glutinosa material, and to explore transferred components and natural ingredients of serum in rats with blood heat and hemorrhage syndrome animal model treated with fresh R. Glutinosa. Results: Catalpol as one of bioactive components was detected in serum of rats with blood heat and hemorrhage syndrome animal model. Meanwhile,three components were found in both fresh R. Glutinosa and it -contained serum. Moreover,fresh R. Glutinosa could prevent the increased amounts of a natural component of serum in rats with blood heat and hemorrhage syndrome animal model. Conclusion .-Captalpol and three unknown ingredients can all be the bioactive components of fresh R. Glutinosa. Furthermore, the mechanism of fresh R. Glutinosa in heat - clearing and blood - cooling efficacy may be related to

  20. Serum bleomycin-detectable iron in patients with thalassemia major with normal range of serum iron.

    Directory of Open Access Journals (Sweden)

    Han,Khin Ei

    1995-06-01

    Full Text Available "Free" iron, a potentially radical-generating low mass iron, and not found in normal human blood, was increased in the serum of blood-transfused thalassemia major patients seen in the Yangon General Hospital, Yangon, Myanmar (Burma. The low mass iron was detected by the bleomycin assay. Fifty-one blood samples were analyzed (from 28 males and 23 females. High "free" iron was detected in 47 sera samples from thalassemia patients. Serum ferritin, which reflects the body store iron, was higher than the normal range (10-200 ng/ml in 49 patients. On the other hand, serum iron of 39 sera samples fell within the normal range (50-150 micrograms/dl. Four were less than 50 micrograms/dl and eight were more than 150 micrograms/dl. Almost all the patients' sera of normal or higher serum iron level contained "free" iron. Thus, almost all the sera from thalassemic patients from Myanmar contain bleomycin-detectable iron, even when serum iron is within the normal range. In developing countries where undernutrition is prevalent (serum albumin in these patients was 3.6 +/- 0.4 g/dl, P < 0.0001 vs. control value of 4.0 - 4.8 g/dl, normal serum iron does not preclude the presence of free iron in the serum.

  1. Cálcio ionizado no soro: estimativa do intervalo de referência e condições de coleta Serum ionized calcium: reference interval estimation and blood collection condictions

    Directory of Open Access Journals (Sweden)

    Adagmar Andriolo

    2004-04-01

    methodology with favorable cost/benefice ratio. The use of this methodology implies in reference interval estimation. OBJECTIVE: To estimate the reference interval for serum ionized calcium, and to evaluate interferences in tourniquet time application, and interferences in sample refrigeration before analysis. MATERIAL AND METHOD: to estimate the reference interval we included the results of 11,320 consecutive calcium ionized determinations accomplished from January 2000 to November 2002; in order to evaluate the effect of sample refrigeration, 16 samples were collected in duplicate, so that one tube was placed in ice bath and the other was maintained in room temperature. To evaluate the effect of tourniquet application time, we collected blood samples from one arm of 6 normal subjects, immediately after, and from the other arm, after 3 minutes of the tourniquet application. The blood was collected in evacuated tubes with gel separator and centrifuged up to 30 minutes after collection. All determinations were performed up to 4 hours after the centrifugation by ion-selective electrode. RESULTS: regarding to the central 95% data distribution, the inferior and superior limits were, respectively, 1.11 (confidence interval of 90%: 1.1 to 1.11 and 1.4mmol/l (confidence interval of 90%: 1.39 to 1.41. No significant differences were detected between results with and without refrigeration and between samples with less than 1 and after 3 minutes of tourniquet application.

  2. Kadar Asam Urat Serum sebagai Biomarker Preeklamsi

    OpenAIRE

    Neli Sumanti; Noormartany; Muhammad Alamsyah; Tiene Rostini

    2013-01-01

    Preeclampsia remains a health problem that becomes one of the causes of maternal deaths besides bleeding and infection. The etiology and pathogenesis of preeclampsia are unclear. Increased serum uric acid levels is seen simultaneously with the increase of blood pressure and occurred before the onset of proteinuria. Therefore, the uric acid can be used as a biomarker. The aim of this study was to analyze the serum uric acid levels between normal and preeclampsia pregnancies. The study was cond...

  3. 槲皮素对力竭运动疲劳大鼠血清自由基代谢的影响%EFFECTS OF QUERCETIN ON BLOOD SERUM FREE RADICAL METABOLISM IN EXHAUSTED FATIGUE RATS

    Institute of Scientific and Technical Information of China (English)

    杨波; 任晓丽; 李翔

    2011-01-01

    目的 探讨槲皮素对力竭运动疲劳大鼠血清自由基代谢和运动能力的影响,为抗疲劳功能食品的研制提供科学依据.方法 雄性SD大鼠随机分为正常安静对照组(对照组)、力竭游泳疲劳组(疲劳组)和力竭游泳疲劳+槲皮素50 mg/kg组(槲皮素组),每组10只.采用一次性力竭游泳运动建立力竭疲劳模型,记录各组大鼠力竭游泳活存时间,测定血清MDA含量、SOD和GSH-Px活力.结果 槲皮素组大鼠力竭游泳活存时间为(348.5±52.4)min,明显长于疲劳组[(171.7±37.1)min,P<0.01];血清MDA含量为(4.4±1.0)nmol/ml,明显低于疲劳组[(6.2±1.9)nmol/ml,P<0.01];SOD和GSH-Px活力分别为(105.0±3.4)和(873.6±72.3)U/ml,均明显高于疲劳组[分别为(80.5±4.2)和(538.6±63.6)U/ml,P<0.01].结论 槲皮素可以提高力竭运动疲劳大鼠血清抗氧化酶活力,减轻脂质过氧化反应,提高大鼠运动能力.%Objective To investigate the effects of Quercetin (QC) on oxidative stress induced by exhausted swimming in rats,so as to provide scientific basis for developing anti-fatigue food.Methods Male SD rats were randomly divided into resting control group, exhausted swimming group, and exhausted swimming with administration of Quercetin group.The content of malondialdehyde (MDA), the activity of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) in blood serum were measured.At the same time the survival time after exhausted swimming was determined.Results The survival time in exhausted swimming ± Quereetin group was longer than exhausted swimming group[(348.5± 52.4)min vs (171.7 ± 37.1 )min, P < 0.01].The content of MDA in exhausted swimming + QC group was lower [(4.4 ± 1.0) nmol/ml vs (6.2 ± 1.9) nmol/ml, P < 0.01], whereas the activity of SOD, and GSH-Px was higher [( 105.0 ± 3.4) U/ml vs (80.5 ± 4.2) U/ml, (873.6 ± 72.3) U/ml vs (538.6 ± 63.6) U/ml respectively, P < 0.01]than in exhausted swimming group.Conclusion Quercetin

  4. Blood pressure

    Science.gov (United States)

    ... the walls of the arteries is called blood pressure. Blood pressure is measured both as the heart contracts, which ... as it relaxes, which is called diastole. Normal blood pressure is considered to be a systolic blood pressure ...

  5. Blood transfusions

    Science.gov (United States)

    ... this page: //medlineplus.gov/ency/patientinstructions/000431.htm Blood transfusions To use the sharing features on this ... several sources of blood which are described below. Blood From the Public (Volunteer Blood Donation) The most ...

  6. Blood Basics

    Science.gov (United States)

    ... Patient Group Links Advocacy Toolkit Home For Patients Blood Basics Blood is a specialized body fluid. It ... about 9 pints. Jump To: The Components of Blood and Their Importance Many people have undergone blood ...

  7. Blood Thinners

    Science.gov (United States)

    If you have some kinds of heart or blood vessel disease, or if you have poor blood flow to your brain, your doctor may recommend that you take a blood thinner. Blood thinners reduce the risk of heart ...

  8. Blood culture

    Science.gov (United States)

    Culture - blood ... A blood sample is needed . The site where blood will be drawn is first cleaned with an antiseptic such ... organism from the skin getting into (contaminating) the blood sample and causing a false-positive result (see ...

  9. [Attitude of blood donors towards cholesterol measurement].

    Science.gov (United States)

    Flesland, O; Botten, G; Solheim, B G; Orjasaeter, H

    1992-05-20

    In analyses of cost-effectiveness it is customary to count knowledge of having a high serum cholesterol level as a negative factor. There is little support for this practice in the literature. We have studied the attitude of 305 Norwegian blood donors towards cholesterol testing. 63% stated that they were interested in their serum cholesterol level, and 40% said they knew their own serum cholesterol level. The attitude towards cholesterol testing was clearly positive, both among men and among women, regardless of age. Only one donor stated that she did not want to have her serum cholesterol tested in conjunction with blood donation. PMID:1509430

  10. Radioimmunoassay for serum 11-deoxy-17-ketosteroids

    International Nuclear Information System (INIS)

    A simplified method for evaluating serum 11-deoxy-17-ketosteroids (11-deoxy-17-KS) equivalent to dehydroepiandrosterone sulfate (DHEAS) has been developed without solvolysis and chromatography. Blood serum or plasma (5 mu l) was added to 1 ml of ethanol, mixed, and centrifuged, and 10 or 20 mu l of the supernatant was evaporated to dryness and incubated with anti-11-deoxy-17-KS antiserum obtained by immunizing a rabbit with DHEA-3.O. CO-BSA which was prepared from DHEA-3.O.COCl and containing DHEAS-7α3H, pepsin-treated human immune serum globulin and bovine serum albumin. Ammonium sulfate was used to separate free from bound DHEAS-7α3H. The accuracy, precision and sensitivity were satisfactory. The blank values could not be differentiated from zero. As the antiserum reacted not only on DHEAS but also on androsterone sulfate and etiocholanolone sulfate, serum 11-deoxy-17KS obtained by the radioimmunoassay expressed nearly the sum of 100 percent of DHEAS, 45 percent of androsterone sulfate and 35 percent of etiocholanolone sulfate in the serum. A good correlation was found between serum 11-deoxy-17-KS and DHEAS obtained by the radioimmunoassay described in a previous paper. The present radioimmunoassay is the simplest method for the evaluation of the concentrations of C19 steroids in the serum. (auth)

  11. Serum Biochemical Profile of Post Partum Metritic Cow

    OpenAIRE

    Magnus P. K.; Lali F. A.

    2009-01-01

    Present study was conducted to find out the relationship between serum biochemical profile and postpartum metritis. Mainly serum glucose, total protein, albumin, albumin globulin ratio, blood urea nitrogen (BUN), creatinine and calcium were studied. Colorimetric method was used for quantitative estimation of biochemical profile. Twenty-seven animals with recent history of calving and subsequent metritis were included in the study. On analysis, serum glucose was found to be 22.3 ± 2.1...

  12. Isolation and characterization of serum albumin from Camelus dromedarius

    OpenAIRE

    Malik, Ajamaluddin; Al-Senaidy, Abdulrahman; SKRZYPCZAK-JANKUN, EWA; JANKUN, JERZY

    2013-01-01

    Serum albumin constitutes 35–50 mg/ml of plasma proteins and performs various physiological activities including the regulation of osmotic pressure on blood, maintaining buffering of the blood pH, carrying different fatty acids and other small molecules, such as bilirubin, hormones, drugs and metal ions, as well as participating in immunological responses. Serum albumin is an extensively used protein in biotechnological and pharmaceutical industries. The camel (Camelus dromedarius) is well ta...

  13. Serum calcium levels are not associated with coronary heart disease

    Directory of Open Access Journals (Sweden)

    Jin Y

    2013-09-01

    Full Text Available Yuelong Jin,* Lianping He,* Quanhai Wang, Yan Chen, Xiaohua Ren, Hui Tang, Xiuli Song, Lingling Ding, Qin Qi, Zhiwei Huang, Jiegen Yu, Yingshui Yao Department of Preventive Medicine, Wannan Medical College, Wuhu, People's Republic of China *These authors contributed equally to this work Background: Numerous studies have reported that low calcium intake is related to a higher prevalence of cardiovascular disease. However, the relationship between serum calcium and coronary heart disease is unclear. The purpose of this study was to compare serum calcium levels in patients with coronary heart disease and those in healthy individuals. Methods: This retrospective, case-control study conducted in the People's Republic of China comprised 380 cases and 379 controls. Serum calcium levels, blood lipids, and anthropometric measurements were measured in both groups. The Student's unpaired t-test or Chi-square test was used to compare differences between cases and controls. Pearson's partial correlation coefficient was used to determine the association between serum calcium, blood lipids, and blood pressure in both groups. Results: Our results indicate that the average level of serum calcium in cases was higher than in controls. Serum calcium levels showed no correlation with any parameter except for triglycerides in either group. Conclusion: Overall, these data suggest that serum calcium has no influence on coronary heart disease or triglyceride levels in the general population. Keywords: serum calcium, hypertension, blood lipids

  14. Serum Mineral Profile in Various Reproductive Phases of Mares

    OpenAIRE

    Farah Ali1, Laeeq Akbar Lodhi*2, Zafar Iqbal Qureshi2, Ijaz Ahmad2 and Riaz Hussain1

    2013-01-01

    The present study was conducted to determine the trace mineral profile in fertile, subfertile and pregnant mares kept under different management conditions. For this purpose the blood samples were collected without anticoagulant from 100 field mares and 100 farm mares for serum separation. All animals were grouped according to their history and rectal examination. Serum manganese levels in pregnant mares were significantly (P

  15. Fluorimetric determination of cholesterol in hypercholesterolemia serum

    Science.gov (United States)

    Lan, Xiufeng; Liu, Jiangang; Liu, Ying; Luo, Xiaosen; Lu, Jian; Ni, Xiaowu

    2005-01-01

    With the increase of people"s living standard and the changes of living form, the number of people who suffer from hypercholesterolemia is increasing. It is not only harmful to heart and blood vessel, but also leading to obstruction of cognition. The conventional blood detection technology has weakness such as complex operation, long detecting period, and bad visibility. In order to develop a new detection method that can checkout hypercholesterolemia conveniently, spectroscopy of cholesterol in hypercholesterolemia serum is obtained by the multifunctional grating spectrograph. The experiment results indicate that, under the excitation of light-emitting diode (LED) with the wavelength at 407 nm, the serum from normal human and the hypercholesterolemia serum emit different fluorescence spectra. The former can emit one fluorescence region with the peak locating at 516 nm while the latter can emit two more regions with peaks locating at 560 nm and 588 nm. Moreover, the fluorescence intensity of serum is non-linear increasing with the concentration of cholesterol increases when the concentration of cholesterol is lower than 13.8 mmol/L, and then, with the concentration of cholesterol increase, the fluorescence intensity decreases. However, the fluorescence intensity is still much higher than that of serum from normal human. Conclusions can be educed from the experiments: the intensity and the shape of fluorescence spectra of hypercholesterolemia serum are different of those of normal serum, from which the cholesterol abnormal in blood can be judged. The consequences in this paper may offer an experimental reference for the diagnosis of the hypercholesterolemia.

  16. Reference values for chinchilla (Chinchilla laniger blood cells and serum biochemical parameters Valores de referência para os parâmetros das células e bioquímica sangüínea da chinchila (Chinchilla laniger

    Directory of Open Access Journals (Sweden)

    Tális de Oliveira Silva

    2005-06-01

    Full Text Available Raising chinchilla (Chinchilla laniger for commercial purpose has increased significantly; however, hematological and serum biochemical reference values have not yet been determined for chinchillas raised in south Brazil. Establishing blood cells and serum biochemistry reference values might be helpful to evaluate health status of chinchillas and might be used as a tool by clinicians. The purpose of this study was to determine the reference values for blood cells and serum biochemistry of Chinchilla laniger. Blood samples were collected by cardiac puncture from 16 adult males, at the time they were killed to remove the fur coat, and from 8 adult males anesthetized with ketamine and xylazine. Blood cell counts and serum biochemistry analysis were performed using standard techniques and the results were expressed as mean ± SEM. Analysis of blood parameters from post-mortem cardiac punctured and from anesthetized chinchillas indicated that blood samples from anesthetized chinchillas had higher PCV, Hemoglobin, MCHC and WBC (P A criação de chinchila (Chinchilla laniger com objetivos comerciais tem crescido muito nos últimos anos. No entanto, os valores de referência para os parâmetros hematológicos e bioquímicos não foram ainda determinados para chinchilas criados no sul do Brasil. O estabelecimento dos valores de referência para esses parâmetros pode servir de auxílio para a avaliação da saúde das chinchilas e servir de auxílio diagnóstico para o clínico. Esse estudo teve como objetivo determinar os valores de referência das células e da bioquímica sangüínea da Chinchilla laniger. As amostras de sangue foram coletadas por meio de punção cardíaca de 16 machos adultos no momento em que os animais foram mortos para remoção da pele, e de 8 machos adultos após anestesia com xylazina e ketamina. A contagem das células sangüíneas e a análise dos parâmetros bioquímicos foram feitas utilizando-se métodos padronizados em

  17. Correlation between Serum T3 Resin Uptake and Serum Lipid Profile

    Directory of Open Access Journals (Sweden)

    Dinarvand, GH. ( MSc

    2015-05-01

    Full Text Available Background and Objective: T3 resin uptake test indicates the availability of thyroxin binding globulin (TBG, which is the carrier of a large amount of T3 and T4 in blood. We aimed to study the relationship between serum T3 Resin Uptake and serum lipids profile. Material and Methods: The participants aged 14.80 ± 38.29 were both males (59; 29.5% and females (141; 70.5 %. T3 Resin Uptake was measured by Immunoassay method and serum lipid profile components (cholesterol, triglycerides, HDL-C, LDL-C, and VLDL were measured by the kit. Results: Significant negative correlation was observed between serum T3 Resin Uptake and cholesterol, triglycerides and LDL-C (P≤ 0.05, but that was not the case for HDL-C and VLDL. Conclusion: Serum T3 Resin Uptake can be evaluated to assess the risk factors related to cardiovascular disorders

  18. Association of ABO and Rh Blood Groups to Blood-Borne Infections among Blood Donors in Tehran-Iran.

    OpenAIRE

    Fatemeh Mohammadali; Aliakbar Pourfathollah

    2014-01-01

    Abstract Background The aim of this study was to investigate the prevalence of hepatitis B, hepatitis C, HIV and syphilis infections in blood donors referred to Tehran Blood Transfusion Center (TBTC), and determine any association between blood groups and blood- borne infections between the years of 2005 and 2011. Methods This was a retrospective study conducted at TBTC. All of the donor serum samples were screened for HBV, HCV, HIV and syphilis by using third generation ELISA kits and RPR te...

  19. 母亲血清乙型肝炎病毒载量及新生儿外周血游离母亲DNA与新生儿乙型肝炎病毒感染的关系%Relationships between serum hepatitis B virus load in mothers, free maternal DNA in peripheral blood of newborns and hepatitis B virus infection of newborns

    Institute of Scientific and Technical Information of China (English)

    魏俊妮; 冯永亮; 王素萍; 李淑珍; 胡婷; 阎娟娟

    2010-01-01

    Objective To study the relationships between serum hepatitis B virus (HBV) DNA level in chronic HBV infected mothers, free maternal DNA in newborns' peripheral blood and HBV infection of newborns. Methods Free maternal DNA in newborns' peripheral blood was amplified by allele-specific polymerase chain reaction (As-PCR) and heminested polymerase chain reaction (heminPCR). Serum HBV DNA of pregnant women were detected by fluorescence quantitative real-time PCR. The relationships between mothers' serum HBV DNA level, mother-to-fetus DNA transfer and newborns HBV infection were analyzed by SPSS 13. 0 software. Results Thirty-six pairs of motherfetus informative cases were selected and free maternal DNA in the peripheral blood was detected in 26newborns (72. 2%). Statistical analysis indicated that mother-to-fetus DNA transfer was not related with HBsAg, HBV DNA DOsitive in newborns (Fisher exact Drobabilities were 0. 278 and 1.000,respectively; both P > 0. 05), while it was related with HBV infection in the peripheral bloodmononuclear cell (PBMC) of newborns (Fisher exact probability was 0. 026, P0. 05). Therisk of HBV DNA positive in newborns increased with mother serum HBV DNA increasing ( total X= 62. 21, P0. 05).Conclusions DNA transfer from HBV infected mother to fetus is related with PBMC HBV infection innewborns, which could be a risk factor of HBV infection in newborns. The risk of serum HBV DNApositive in newborns increases with mother serum HBV DNA level increasing.%目的 探讨慢性HBV感染母亲血清HBV DNA载量及新生儿外周血中游离母亲DNA与新生儿HBV感染的关系.方法 等位基因特异性PCR及半套式PCR技术检测慢性HBV感染母亲及其新生儿外周血中游离母亲DNA.荧光定量PCR对母亲外周血血清中HBV DNA定量检测.数据采用阳性率、X2检验、Fisher精确概率等进行统计学分析.结果 筛选出36对母儿信息病例对,有26个新生儿外周血中检测到游离母亲DNA,占72.2%.母-

  20. Blood Types

    Science.gov (United States)

    ... How Can I Help a Friend Who Cuts? Blood Types KidsHealth > For Teens > Blood Types Print A A ... or straight hair instead of curly. ...Make Eight Blood Types The different markers that can be found in ...

  1. Blood Types

    Science.gov (United States)

    ... confidence to respond in emergency situations with the skills that can help to save a life. Learn more » Red Cross Information Donating Blood Learn About Blood Hosting a Blood Drive For Hospitals Engage with Us About Us Media ...

  2. Ferritin. Radioimmunological determination in serum and clinical significance

    International Nuclear Information System (INIS)

    Ferritin is an iron storage protein which has been shown to be present in blood serum only recently. An immunoradiometric determination of ferritin in 324 subjects with different iron stores is reported. In healthy men and women a ferritin concentration of 131 μg/l (SD: 1.59) and 67 μg/l (SD: 1.79) was found respectively. In male and female blood donors as well as patients with iron deficiency and iron overload significant differences of serum ferritin concentration could be demonstrated. In clinical practice the determination of serum ferritin is a valuable method for the estimation of body iron stores. (orig.)

  3. Serum and urine trace metals in ketosis

    Energy Technology Data Exchange (ETDEWEB)

    Burch, R.E.; Hiner, C.; Sullivan, J.F.

    1974-01-01

    Three groups of male rats were housed in metabolic cages. The control group was fed ad libitum, a second group was fasted and diabetes was induced in the third group by the subcutaneous injection of alloxan. After 48 hours all animals were bled and livers, which were quick-frozen in situ, were obtained. Fasted and diabetic animals were ketotic as manifested by lowering urinary pH and elevated urinary and blood acetoacetate and ..beta..-hydroxybutyrate. Blood and hepatic content of acetate was elevated in diabetic versus control rats. Blood and hepatic content of acetate was not increased in fasted animals. In fasted rats total urinary Zn and Cu were decreased and Mg and Ca did not differ significantly from control values. In diabetic rats total urinary excretion of Mg was increased whereas urinary Zn, Cu and Ca levels did not differ significantly from control values. In fasted animals only serum Zn was diminished. In diabetic rats serum Cu and Ca were decreased and serum Mg was increased while serum Zn was unchanged. Thus, the ketosis of fasting differs from the ketosis of diabetes in several of its metabolic manifestations. 11 references, 9 figures.

  4. Crocodylus siamensis serum and macrophage phagocytic activity.

    Science.gov (United States)

    Aree, Kalaya; Siruntawineti, Jindawan; Chaeychomsri, Win

    2011-12-01

    Antimicrobial activity of sera from many crocodilian species has been recognized. This activity was proposed to be mediated, at least in part, by complement. Due to the fact that complement proteins have different functions in the immune system, they may be involved in phagocytic process of phagocytes. In the present study, the effects of Siamese crocodile serum on phagocytic activity of macrophages as well as the possible involvement of complement in this process were examined. The results showed increases in the phagocytosis of both Escherichia coli and to a lesser extent, Staphylococcus aureus upon incubation of murine macrophage cell line with fresh crocodile serum (FS). Similar to FS, other crocodile blood products, including freeze dried serum (DS) and freeze dried whole blood (DWB) exhibited phagocytosis-enhancing property. However the ability of DWB to enhance phagocytosis was less efficient than that of FS and DS, suggesting that serum factors were involved in this process. Treatment of FS with heat at 56 degrees C for 30 min deteriorated the effect of FS on bacterial uptake of macrophages, suggesting that complement proteins play a role in the modulation of the phagocytic process. Collectively, the results of the present study suggested that crocodile serum enhances the macrophage phagocytic activity through complement activity and, therefore, may be taken as an alternative medicine for supporting the human immune responses. PMID:22619919

  5. Elemental analysis of human serum and serum protein fractions by thermal neutron activation

    International Nuclear Information System (INIS)

    Some applications of thermal neutron activation for the determination of elemental contents in human serum and human serum protein fractions are presented. Firstly total serum is dealt with, secondly serum protein fractions obtained by gel filtration are described. A brief review on the role of (trace) elements in human health and disease and a compilation of literature data for elemental contents in human serum, as obtained by neutron activation techniques, are given. The most important sources of statistical and systematic errors are evaluated. Results for the contents of sodium, potassium, magnesium, bromine, iron, copper, zinc, selenium, rubidium, cesium and antimony in serum are given, with emphasis on control of accuracy and precision. The possible relation between selenium in blood and cancer occurrence in humans is discussed. The results of elemental analyses from cancer patients and from a patient receiving a cytostatic treatment are presented. A survey of literature results for the determination of protein-bound elemental contents in serum is presented. Subsequently, results from a study on the behaviour of elements during gel filtration are discussed. Gel-element and protein-element interactions are studied. Finally the protein-bound occurrence of trace elements in human serum is determined by gel filtration and neutron activation analysis. Results for both desalting and fractionation are given, for the elements bromine, copper, manganese, vanadium, selenium, zinc, rubidium, iron and iodine. (Auth.)

  6. Effect of aerobic exercise on ambulatory blood pressure, serum uric acid and HDL in young and middle-aged male patients with mild essential hypertension%运动对中青年高血压患者动态血压和血清尿酸、高密度脂蛋白水平的影响

    Institute of Scientific and Technical Information of China (English)

    申秀玲; 李平; 张菲斐

    2013-01-01

    Objective To investigate the effect of aerobic exercise on ambulatory blood pressure,serum uric acid and HDL in young and middle-aged male patients with mild essential hypertension.Methods Sixty patients who had mild hypertension were randomly divided into control group and observed group (n =30).Control group were administrated nifedipine (10 mg,bid) and observed group received aerobic exercise (30-60 minutes)everyday for 12 weeks.Change of blood pressure in 24 hours,serum uric acid and blood lipids of sixty subjects were measured before and after a 12-week exercise training program.Date were analyzed with statistical methods.Results After 12 weeks,the systolic/diastolic blood pressure decreased significantly in two groups.SUA reduced,with 20.96/23.33 μmol/L.TG,TC,LDL-C reduced,HDL-C increased,with 0.27/0.22 mmol/L (P < 0.05).After treatment,there were no statistical significance with SBP,DBP,SUA and blood lipids between the two groups.Conclusions Aerobic exercise may decrease blood pressure and serum uric acid and improve lipid metabolism.The effect is equivalent to drug treatment.%目的 观察长期有氧运动对男性中青年高血压患者动态血压、血清尿酸和高密度脂蛋白(HDL)的影响.方法 将60例轻度男性高血压病患者随机分为对照组和观察组,对照组给予硝苯地平缓释片(10 mg,每日2次),观察组采用运动疗法(每天进行30 ~60 min的有氧运动),并分别于治疗前、治疗12周末各做24 h动态血压监测(ABPM)1次,并于治疗前后分别测定血尿酸(SUA)和血脂.结果 治疗3个月后进行比较,对照组、观察组患者的收缩压(SBP)、舒张压(DBP)均明显降低(P<0.05);SUA均明显降低,分别下降20.96、23.33 μmol/L;三酰甘油(TG)、总胆固醇(TC)、低密度脂蛋白胆固醇(LDL-C)降低,高密度脂蛋白胆固醇(HDL-C)升高,分别增加0.27、0.22 mmol/L(P<0.05),治疗后两组患者之间SBP、DBP、SUA、血脂比较差异无统计学意义(P>0.05).

  7. Serum histidine in rheumatoid arthritis: a family study

    OpenAIRE

    J Kirkham; Lowe, J.; Bird, H. A.; Wright, V

    1981-01-01

    We have compared free serum histidine in patients with rheumatoid arthritis, their blood relatives, and their non-blood relatives. The hypohistidinaemia of rheumatoid arthritis is acquired with the disease and does not provide a biochemical marker of those at risk.

  8. Serum hyaluronan levels in oral cancer patients

    Institute of Scientific and Technical Information of China (English)

    2008-01-01

    Background Hyaluronan(HA)is most likely associated with tumor invasion and metastasis.Studies have shown that HA levels are often increased in serum of patients with various malignant tumors.The purpose of this study was to determine the levels of serum hyaluronan in patients with oral cancer and evaluate the value of serum HA in adjuvant diagnosis,staging and monitoring treatment response in these patients.Methods Eighty-four hospitalized patients with oral cancer,65 patients with benign tumors in the oral and maxillofacial region and 67 healthy individuals were included in this investigation.Venous blood was collected from these patients and the healthy individuals before therapy.One week after therapy,venous blood was collected once again in 43 patients with oral cancer.Serum samples were obtained and serum HA levels examined.Results The serum HA concentration was significantly higher in oral cancer patients than in patients with benign tumors and in healthy controls(P<0.05).The serum HA level in patients with stages Ⅲ and Ⅳ disease was higher than in patients with stages Ⅰ and Ⅱ disease,but there was no significant difference in the HA level between stages Ⅰ and Ⅱ nor between stages Ⅲ and Ⅳ(P>0.05).After a complete treatment the HA levels in patients with oral cancer became lower than before treatment,but the difference was not significant(P>0.05).Conclusions The results of this study suggest that the determination of HA levels may provide additional information in diagnosis of oral cancer,but its usefulness as an adjunct in clinical staging and in monitoring treatment response was limited.

  9. Clinical significance of determination of serum leptin levels in patients with type 2 diabetes mellitus

    International Nuclear Information System (INIS)

    Objective: To investigate the relationship between levels of serum leptin and levels of blood sugar, lipid as well as degree of obesity in patients with type 2 diabetes mellitus (DM2). Methods: Serum leptin levels were determined with RIA in 42 patients with DM2 and 38 controls. Results: The serum leptin levels in DM2 patients were significantly higher than those in controls (P <0.001) and were positively correlated with serum INS, TC, TG, LDL-C levels as well as BMI. Conclusion: High level of serum leptin was associated with obesity, high blood lipid levels and insulin resistance (IR). (authors)

  10. RT-PCR em pools de soros sangüíneos para o diagnóstico da infecção aguda e de animais persistentemente infectados pelo vírus da diarréia viral bovina RT-PCR in pools of bovine blood serum to detect acute infection and persistently infected animals with bovine viral diarrhea virus

    Directory of Open Access Journals (Sweden)

    D. Pilz

    2007-02-01

    Full Text Available Utilizou-se a técnica da RT-PCR para a detecção da região 5' UTR do genoma do vírus da diarréia viral bovina (BVDV em pools de soros sangüíneos provenientes de um rebanho, constituído por 226 animais, que apresentava distúrbios da reprodução. A partir das amostras individuais de soro e de acordo com a categoria dos animais e o número de animais por categoria foram formados 10 pools (A a J de soros. A primeira avaliação revelou a amplificação de um produto com 290pb nas reações referentes aos grupos D (35 vacas e H (25 bezerros lactentes que, após o desmembramento em amostras individuais, resultou na identificação de 11 vacas lactantes e 12 bezerros em amamentação positivos. Para a identificação de animais persistentemente infectados (PI entre os 23 positivos na primeira avaliação, realizou-se a segunda colheita de soros sangüíneos, três meses após. A RT-PCR das amostras individuais de soro revelou resultado positivo em cinco bezerros. Em dois, foi possível isolar o BVDV em cultivo de células MDBK. A especificidade das reações da RT-PCR foi confirmada pelo seqüenciamento dos produtos amplificados a partir do soro de uma vaca com infecção aguda, de um bezerro PI e das duas amostras do BVDV isoladas em cultivo celular. A utilização da RT-PCR em pools de soros sangüíneos demonstrou ser uma estratégia rápida de diagnóstico etiológico e de baixo custo tanto para a detecção de infecção aguda quanto de animais PI.The 5' untranslated region of the bovine viral diarrhea virus (BVDV genome was detected by RT-PCR assay in pools of blood sera samples collected from a cattle herd (n=226 animals with reproductive failures. Based on the classes of animal and the number of animals per class, the individual blood serum samples were distributed in 10 sera pools (A to J. During the first evaluation a 290bp amplicon was amplified in reactions from groups D (35 cows and H (25 sucking calves. The individual analysis

  11. [Changes in the L-serine and L-threonine dehydrogenase activities in the blood serum of those who worked in the cleanup of the aftermath of the accident at the Chernobyl Atomic Electric Power Station who became ill with chronic acalculous cholecystitis].

    Science.gov (United States)

    Komarenko, D I; Soboleva, L P; Ovsiannikova, L M; Kadiuk, E N; Shvaĭko, E A; Nosach, E V

    1999-06-01

    Activity was studied of blood serum plasmic enzymes L-serine and L-threonine dehydrogenazes (SDG and ThDG) in 92 liquidators of aftermath of the Chernobyl atomic power plant breakdown, presenting with chronic non-calculous cholecystitis during the stage of moderately severe exacerbation with no clinical and laboratory and sonographic signs of affection of the liver. A quarter of the examinees demonstrated an increased activity of the enzymes under study, which fact is regarded by the authors as a preclinical sign of reactive hepatitis. Recommendations are given as to the outpatient registration and prophylactic management and therapy of those persons having taken part in the elimination of the effects of the Chernobyl accident, presenting with biliary pathologies. PMID:10476630

  12. Serum cytokine levels in Kleine-Levin syndrome

    DEFF Research Database (Denmark)

    Kornum, Birgitte Rahbek; Rico, Thomas; Lin, Ling;

    2015-01-01

    unknown. The objective of this study was to determine serum cytokine levels in patients with KLS during and between episodes. PATIENTS/METHODS: Fifty-two typical KLS patients were included in the study of whom 17 patients donated blood samples both during and between episodes. Blood samples were collected...... patients and age- and gender matched healthy controls (n = 8/group) whose blood samples were all collected and processed at the same day; asymptomatic KLS patients had significantly higher levels of serum sVCAM1 cytokine compared to healthy controls. CONCLUSION: These data suggest that KLS episodes are not...

  13. Blood composition of the reindeer . II. Blood chemistry

    OpenAIRE

    Mauri Nieminen; Jouni Timisjärvi

    1983-01-01

    The blood chemical composition of 578 semi-domestic reindeer were investigated in respect to age, season, calving and nutrition in Northern Finland during 1973-79. The weight gain was maximally 400 g/day at an age of 4-8 weeks as also reflected by high serum thyroxine (T4), alkaline phosphatase (SAP), creatine phosphokinase (CPK) and blood glucose values. Low SAP activity in winter indicated a cessation of growth. The pH of the venous blood was 7.35 and the clotting activity very high (21 sec...

  14. Pediatric patient with Bombay blood group: A rare case report

    OpenAIRE

    Sudeshna Bhar (Kundu); Anisha De; Anindita Saha; Chiranjib Bhattacharyya

    2015-01-01

    Bombay blood group is a rare blood group in which there is the absence of H antigen and presence of anti-H antibodies. At the time of blood grouping, this blood group mimics O blood group due to the absence of H antigen, but it shows incompatibility with O group blood during cross matching. Serum grouping or reverse grouping are essential for confirmation of the diagnosis. Patients carrying this blood group can receive blood only from a person with this blood group. Reported cases of anesthes...

  15. Serum Interleukin 17 Levels in Patients with Crohn's Disease: Real Life Data

    OpenAIRE

    Abdurrahman Sahin; Turan Calhan; Mustafa Cengiz; Resul Kahraman; Kubra Aydin; Kamil Ozdil; May Korachi; H. Mehmet Sokmen

    2014-01-01

    The aim of this study was to investigate serum IL17 levels in patients with Crohn's disease (CD) and to investigate the relationship between serum IL17 levels with disease activity. Methods. Fifty patients with CD and sex- and age-matched 40 healthy controls were included in the study. The serum IL17 levels, complete blood count, blood chemistry, erythrocyte sedimentation rate (ESR), and C-reactive protein (CRP) levels were measured, and Crohn's disease activity was calculated using Crohn's d...

  16. Serum Zn levels in dysphagic patients who underwent endoscopic gastrostomy for long term enteral nutrition

    Directory of Open Access Journals (Sweden)

    Carla Adriana Santos

    2014-02-01

    Full Text Available Background and aims: Dysphagic patients who underwent endoscopic gastrostomy (PEG usually present protein-energy malnutrition, but little is known about micronutrient malnutrition. The aim of the present study was the evaluation of serum zinc in patients who underwent endoscopic gastrostomy and its relationship with serum proteins, whole blood zinc, and the nature of underlying disorder. Methods: From patients that underwent gastrostomy a blood sample was obtained minutes before the procedure. Serum and whole blood zinc was evaluated using Wavelength Dispersive X-ray Fluorescence Spectroscopy. Serum albumin and transferrin were evaluated. Patients were studied as a whole and divided into two groups: head and neck cancer (HNC and neurological dysphagia (ND. Results: The study involved 32 patients (22 males, aged 43-88 years: HNC = 15, ND = 17. Most (30/32 had low serum zinc, 17/32 presented normal values of whole blood zinc. Only two, with traumatic brain injury, presented normal serum zinc. Serum zinc levels showed no differences between HNC and ND patients. There was no association between serum zinc and serum albumin or transferrin. There was no association between serum and whole blood zinc. Conclusions: Patients had low serum zinc when gastrostomy was performed, similar in HNC and ND, being related with prolonged fasting and unrelated with the underlying disease. Decrease serum zinc was unrelated with low serum proteins. Serum zinc was more sensitive than whole blood zinc for identifying reduced zinc intake. Teams taking care of PEG-patients should include zinc evaluation as part of the nutritional assessment, or include systematic dietary zinc supply.

  17. 利用母血中胎儿有核红细胞结合血清筛查和三维超声无创性产前诊断唐氏综合征%Noninvasive prenatal screening of Down's syndrome by fetal nucleated erythrocytes detection in maternal blood combined with serum screening and three-dimensional ultrasound

    Institute of Scientific and Technical Information of China (English)

    相文佩; 温子娜; 水丽君; 徐晓燕; 陈汉平

    2011-01-01

    目的:利用母外周血中胎儿有核红细胞结合血清三联筛查及三维超声,建立快速无创性产前诊断唐氏综合征的有效模式.方法:早、中期孕妇共670例,采取血清三联筛查结合三维超声和病史选取唐氏高危孕妇,抽取高危孕妇的外周血,流式细胞术富集母血中的胎儿有核红细胞(Fetal Nucleated Red Blood Cells,FNRBCs);次日进行多重引物原位杂交(mutiprimed in situ labeling,multi-PRINS)检测胎儿细胞21号染色体与Y染色体.结果:通过血清三联筛查和三维超声结合病史筛选出高危孕妇24例,高危孕妇在两日内即可确诊,24例中诊断23例染色体正常胎儿,包括男胎12例、女胎11例;诊断1例男性唐氏综合征胎儿.24例标本检测结果和实际胎儿核型符合.结论:血清三联筛查、超声检查结合病史筛选出高危孕妇,然后利用母血中胎儿有核红细胞进行多重引物原位杂交检测细胞染色体,可作为快速、无创性产前诊断唐氏综合征的有效模式,并可为其他胎儿染色体基因异常或者宫内感染的无创性产前诊断提供参考.%Objective: To establish a rapid and effective noninvasive prenatal diagnostic model of Down's syndrome by fetal nucleated erythrocytes detection in maternal blood combined with serum triple screening and three -dimensional ultrasound. Methods:670 pregnant women of early, middle and late pregnancy were selected, the high risk pregnant women of Down's syndrome were chosen by senun triple screening combined with three - dimensional ultrasound and medical history; the peripheral blood samples of high risk pregnant women were abstracted, flow cytometry was used to enrich fetal nucleated erythrocytes in maternal blood; on the following day, multiple - primer in situ hybridization was used to detect Y chromosome and 21 chromosome in fetal cells. Results:24 high risk pregnant women of Down's syndrome were screened out by serum triple screening combined with

  18. Artificial blood

    Directory of Open Access Journals (Sweden)

    Sarkar Suman

    2008-01-01

    Full Text Available Artificial blood is a product made to act as a substitute for red blood cells. While true blood serves many different functions, artificial blood is designed for the sole purpose of transporting oxygen and carbon dioxide throughout the body. Depending on the type of artificial blood, it can be produced in different ways using synthetic production, chemical isolation, or recombinant biochemical technology. Development of the first blood substitutes dates back to the early 1600s, and the search for the ideal blood substitute continues. Various manufacturers have products in clinical trials; however, no truly safe and effective artificial blood product is currently marketed. It is anticipated that when an artificial blood product is available, it will have annual sales of over $7.6 billion in the United States alone.

  19. [Indirect hemagglutination inhibition reactions as a method of titrating immune serums. V. Application of the reaction to the quantitative determination of immunoglobulins A, M and G in human blood sera].

    Science.gov (United States)

    Konikova, R E; Semenova, B N; Noskov, F S; Shakhanina, K L; Malkina, L A

    1975-01-01

    The authors present the results of using the indirect hemagglutination inhibition test (IHIT) for quantitative determination of A, M and G immunoglobulins in the blood sera of humans in comparison with the method of radial immunodiffusion in agar (RID) after Mancini. The results of IHIT were no less precise and reproducible than those of RID. The significance of the correlation coefficient of grades after Spirman constituted greater than 99.9% for both tests. On this basis a conclusion was made that, having a number of advantages over RID, IHIT could be recommended for quantitative titration of immunoglobulins of various classes. PMID:804778

  20. Helicobacter pylori infection according to ABO blood group among blood donors in Kosovo

    OpenAIRE

    Bukurije Zhubi; Zana Baruti-Gafurri; Ymer Mekaj; Mimoza Zhubi; Idriz Merovci; Iliriane Bunjaku; Valdete Topciu; Emine Devoli-Disha

    2011-01-01

    Introduction: Numerous studies have reported a high prevalence of Helicobacter pylori infection among healthy and non-healthy persons in different places. The Aim of the study is to investigate the seroprevalence of H. pylori infection among Kosovo’s Blood donor associated with ABO/Rhesus blood group.Methods: 671 blood donors are tested for H. pylori antibodies and results are classifi ed by way of donation, age, gender, blood groups and education level. Serum antibodies are analyzed by Enzym...

  1. Concentraciones de inmunoglobulinas séricas y calostrales de vacas selenio-deficientes y en el suero sanguíneo de sus terneros Serum and colostrum immunoglobulin concentrations from selenium deficient cows and in the blood of their calves

    Directory of Open Access Journals (Sweden)

    V. Leyan

    2004-12-01

    Full Text Available Se estudió el efecto de una dieta selenio-deficiente en vacas gestantes sobre la concentración de inmunoglobulinas en el calostro y en el suero sanguíneo de las crías en sus primeras horas de vida. Para ello se trabajó con 12 vacas gestantes (7-8 meses entre 4-9 años de edad, mantenidas en estabulación permanente y sometidas a una dieta deficiente en selenio (0.04 ppm de materia seca, constituida por heno y un alimento concentrado comercial seleniodeficiente. Un grupo de seis vacas fueron suplementadas con selenato de bario (1 mg Se/kg, s.c.. El balance de selenio fue medido a través de la actividad de GSH-Px eritrocítica. La medición de inmunoglobulinas en el suero y calostro de las vacas y en el suero de los terneros se realizó por inmunodifusión radial. Los resultados demostraron que no existen diferencias (PThe colostrum immunoglobulin concentrations of selenium-deficient cows and in the blood of their calves were studied in twelve Friesian cows of 4-9 year old. The animals were 7 months pregnant at the beginning of the experience and it were fed with a selenium deficient diet consisted in hay and a commercial concentrate (diet 0.04 ppm dry matter and kept under permanent housing condition with water ad libitum. The cows were allocated into two homogeneous groups of six animals each, selenium supplemented (Se-S and selenium deficient (Se-D and the animals of the Se-S group were supplemented with barium selenate (1 mg/Se/kg s. c. ± 45 days before calving, whilst the Se-D group received nothing. The balance of selenium was assessed by GSH-Px activity in blood and the concentration of immunoglobulin in serum and colostrums by immunodiffusion radial method. Selenium deficiency did not affect serum and colostrum total IgG, IgM and IgA concentration in cows (P<0.05. However, the IgG1 colostrum concentrations decreased in selenium supplemented cows (P<0.05. On the other hand, the nutritional deficiency of selenium did not affect total

  2. Serum pneumoproteins in firefighters

    NARCIS (Netherlands)

    Greven, Frans; Krop, Esmeralda; Burger, Nena; Kerstjens, Huib; Heederik, Dick

    2011-01-01

    Serum Clara cell protein (CC16) and surfactant-associated protein A (SP-A) were measured in a cross-sectional study in 402 firefighters. For the population as a whole, no associations were detected between serum pneumoproteins and smoke exposure. SP-A levels were increased in symptomatic subjects ex

  3. Serum YKL-40

    DEFF Research Database (Denmark)

    Mylin, Anne K; Abildgaard, Niels; Johansen, Julia S; Heickendorff, Lene; Kreiner, Svend; Waage, Anders; Turesson, Ingemar; Gimsing, Peter

    2015-01-01

    for SRE and at 9 and 24 months for radiographic progression. Elevated serum YKL-40 was seen in 47% of patients and associated with high-risk disease (International Staging System stage III; p < 0.001), increased bone resorption (serum CTX/MMP; p < 0.001) and early radiographic progression at 9 months...

  4. Community-Based Mind-Body Meditative Tai Chi Program and Its Effects on Improvement of Blood Pressure, Weight, Renal Function, Serum Lipoprotein, and Quality of Life in Chinese Adults With Hypertension.

    Science.gov (United States)

    Sun, Jing; Buys, Nicholas

    2015-10-01

    Obesity, metabolic syndrome, dyslipidemia, and poor quality of life are common conditions associated with hypertension, and incidence of hypertension is age dependent. However, an effective program to prevent hypertension and to improve biomedical factors and quality of life has not been adequately examined or evaluated in Chinese older adults. This study aims to examine the effectiveness of a Tai Chi program to improve health status in participants with hypertension and its related risk factors such as dyslipidemia, hyperglycemia, and quality of life in older adults in China. A randomized study design was used. At the conclusion of the intervention, 266 patients remained in the study. Blood pressure and biomedical factors were measured according to the World Diabetes Association standard 2002. A standardized quality-of-life measure was used to measure health-related quality of life. It was found that a Tai Chi program to improve hypertension in older adults is effective in reducing blood pressure and body mass index, maintaining normal renal function, and improving physical health of health-related quality of life. It did not improve existing metabolic syndrome levels, lipid level (dyslipidemia) or fasting glucose level (hyperglycemia), to prevent further deterioration of the biomedical risk factors. In conclusion, Tai Chi is effective in managing a number of risk factors associated with hypertension in Chinese older adults. Future research should examine a combination of Tai Chi and nutritional intervention to further reduce the level of biomedical risks. PMID:26251005

  5. Serum Hepcidin Levels Are Associated With Obesity but Not Liver Disease

    OpenAIRE

    Vuppalanchi, Raj; Troutt, Jason S; Konrad, Robert J.; Ghabril, Marwan; Saxena, Romil; Bell, Lauren N; Kowdley, Kris V.; Chalasani, Naga

    2013-01-01

    Objective Hepcidin is regulated by anemia and inflammation. It is primarily expressed in the liver but studies have reported its expression in adipose tissue. We investigated the relationship between BMI and serum hepcidin and also examined the relationship between liver histology and serum hepcidin in the morbidly obese. Design and Methods Serum and liver tissue from patients undergoing bariatric surgery (bariatric cohort, n=105) and serum from healthy blood donors (n=60) were used to conduc...

  6. Assessment of Serum Vitamin D Levels in Hypertensive Patients

    International Nuclear Information System (INIS)

    High blood pressure is very prevalent worldwide, as well as vitamin D deficiency. There are several observation data that support the concept that vitamin D is involved in the pathogenesis of hypertension. Also some clinical data demonstrate positive effect of vitamin D therapy on hypertension. We studied serum levels of vitamin D and its association with blood pressure in 30 hypertensive patients and 20 age and socioeconomic status matched healthy controls. There was no statistically significant difference in serum vitamin D levels in the group of patients compared with controls. In patients group, patients with body mass index (BMI) > 30 kg/m2 showed statistically significant decrease in serum vitamin D levels as compared to those with BMI ≤ 30 kg/m2 (P = 0.021). There was no significant association between serum vitamin D levels and age, systolic and diastolic blood pressure in the group of patients. A negative correlation between serum vitamin D levels and BMI was found in patients group (P = 0.045). In conclusion, no association between serum levels of vitamin D and blood pressure in hypertensive patients, but it is statistically associated with BMI. Further large scale studies are needed putting in consideration the effect of genetic factor

  7. The reference range of serum, plasma and erythrocyte magnesium

    Directory of Open Access Journals (Sweden)

    Suzanna Immanuel

    2006-12-01

    Full Text Available The interest in the clinical importance of serum magnesium level has just recently begun with the analysis and findings of abnormal magnesium level in cardiovascular, metabolic and neuromuscular disorder. Although the serum level does not reflect the body magnesium level, but currently, only serum magnesium determination is widely used. Erythrocyte magnesium is considered more sensitive than serum magnesium as it reflects intracellular magnesium status. According to NCCLS (National Committee for Clinical Laboratory Standards every laboratory is recommended to have its own reference range for the tests it performs, including magnesium determination. The reference range obtained is appropriate for the population and affected by the method and technique. This study aimed to find the reference range of serum and plasma magnesium and also intracellular magnesium i.e. erythrocyte magnesium by direct method, and compare the results of serum and plasma magnesium. Blood was taken from 114-blood donor from Unit Transfusi Darah Daerah (UTDD Budhyarto Palang Merah Indonesia (PMI DKI Jakarta, consisted of 57 male and 57 female, aged 17 – 65 years, clinically healthy according to PMI donor criteria. Blood was taken from blood set, collected into 4 ml vacuum tube without anticoagulant for serum magnesium determination and 3 ml vacuum tube with lithium heparin for determination of erythrocyte and plasma magnesium Determination of magnesium level was performed with clinical chemistry auto analyzer Hitachi 912 by Xylidil Blue method colorimetrically. This study showed no significant difference between serum and heparinized plasma extra cellular magnesium. The reference range for serum or plasma magnesium was 1.30 – 2.00 mEq/L and for erythrocyte magnesium was 4.46 - 7.10 mEq/L. (Med J Indones 2006; 15:229-35Keywords: Reference range, extracellular magnesium, intracellular magnesium

  8. Blood smear

    Science.gov (United States)

    ... osmotic fragility ) Deficiency of an enzyme called lecithin cholesterol acyl transferase Abnormalities of hemoglobin , the protein in ... sickle and Pappenheimer Red blood cells, target cells Formed elements of blood References Bain BJ. The peripheral ...

  9. EFFECT OF ADIPECTOMY ON BLOOD GLUCOSE, SERUM LEPTIN AND ADIPONECTIN IN RATS WITH ALIMENTARY OBESITY%脂肪切除术对营养性肥胖大鼠血糖、瘦素及脂联素的影响

    Institute of Scientific and Technical Information of China (English)

    杨柳; 刘肃; 匡瑞霞; 陈璐

    2012-01-01

    Objective To study the influence of adipectomy on blood glucose, leptin and adiponectin in rats with alimentary obesity. Methods An obese model was created in male rats by feeding with high fat diet, which were then divided into operartion group and control group. The rats in operation group underwent resection of fat surrounding epididymis and kidney, and those in the control group, seperation of fat only was done. The differences of the levels of blood glucose, leptin and adiponectin were measured and compared between the two groups, pre- and postoperatively. Results The concentration of all the above items was significantly different on day 15 after surgery between the two groups, except leptin (t = 4. 25 -13. 34, P<0. 01). Conclusion Resection of a great quantity of fat can lower the blood sugar level in rats with alimentary obesity, raise serum adiponectin, and reduce serum leptin within a short period of time.%目的 研究脂肪切除对营养性肥胖大鼠血糖、瘦素及脂联素的影响.方法 以高脂饮食制备成年雄性肥胖大鼠模型,随机分为手术组与对照组,手术组大鼠实施睾丸旁、肾周脂肪切除术,对照组行阴性对照手术,仅分离双侧睾丸旁、肾周脂肪,术后2、15 d测量并比较两组手术前后空腹血糖、血清瘦素及脂联素的差值.结果 手术组大鼠除术后15d血清瘦素差值与对照组比较差异无显著性外,术后2、15 d其余各项指标比较差异均有显著性(t=4.25~13.34,P<0.01).结论 手术切除大量脂肪可以降低营养性肥胖大鼠的血糖水平,提高脂联素水平,并在短期内降低血清瘦素水平.

  10. Correlative study on blood perfusion and hemodynamics parameters observed by ultrasonography and serum creatinine levels in patients with renal transplantation%超声观察移植肾血流灌注及动力学参数与血清肌酐水平的相关性研究

    Institute of Scientific and Technical Information of China (English)

    李晓萌; 李博慧; 朱军; 赵洋; 曹晔; 杜巍

    2012-01-01

    Objective To study the correlation of blood perfusion and hemodynamics parameters observed by ultrasonography and serum creatinine (SCr) levels in patients with renal transplantation. Methods One hundred and twenty-six patients who were after renal transplantation 1 year were enrolled in this study. They were divided into normal renal function group (30 cases) and abnormal renal function group (96 cases) according to serum creatinine level. The morphology,size and structure of transplanted kidney were observed by two-dimensional ultrasound. The renal arterial blood flow perfusion was observed by color Doppler flow imaging. The spectral pattern was observed by PW Doppler. The systolic maximum velocity ( Vp ), Diastolic minimum velocity ( Vmin ) , mean velocity ( Vmean ), resistance index ( RI) and pulsatility index ( PI) of main renal artery and interlobar artery were measured. Results The ultrasonic manifestations of normal renal function group showed uniform echo in transplantation renal cortex, internal structure was clear, the renal blood vessels distributed as "branch-like" , abundant blood flow signal was found.Spectrum showed consistent forward blood flow with low resistance in whole cardiac cycle. There were 96 cases of medullary edema in abnormal renal function group, 30 cases of those showed enhancement solid echo and regular edge. Compared with normal renal function group, Vp, Vmin and Vmean of main renal artery and interlobar artery in abnormal renal function group were decreased, RI and PI were increased (P <0.05). Conclusion The blood perfusion and hemodynamics parameters observed by ultrasonography can provide a imaging evidence for function change of transplanted kidney.%目的 超声观察移植肾血流灌注及动力学参数与血清肌酐水平的相关性研究.方法 选取肾移植术后1年的126例患者,根据血清肌酐水平分为肾功能正常组30 例及异常组96 例.二维超声观察移植肾形态、大小、结构,彩色多普

  11. 脑血流低灌注老龄大鼠中脑皮质血流的改变与血清总胆固醇和高密度脂蛋白的动态变化%Dynamic changes of cortical blood flow and serum total cholesterol and high density lipoprotein in brains of aging rat during cerebral hypoperfusion

    Institute of Scientific and Technical Information of China (English)

    王林辉; 田茗源; 滕志朋; 王晨; 李昱

    2012-01-01

    目的:通过建立脑血流低灌注模型,观察老龄大鼠脑血流的变化以及在脑血流低灌注下血清中总胆固醇(Total cholesterol,TC)和高密度脂蛋白(High density lipoprotein,HDL)的动态变化.方法:采用持久性双侧颈总动脉结扎法(2Vo)致老龄大鼠脑血流灌注不足,测定术后7、14、21、28d大鼠脑皮质血流;检测、比较术后不同时间段大鼠血清中TC和HDL浓度差异.结果:术后第14天大鼠脑颞区血流出现明显减少;术后21、28d大鼠脑局部皮质血管有再生侧支形成,大鼠脑颞区血流仍未恢复;术后第14天血清中HDL、TC含量明显高于假手术组(P<0.05),随着缺血时间延长,又逐渐降低.结论:血清TC和HDL浓度在脑缺血灌注不足的不同时间段经历了先增强后减弱的动态变化,提示脑血流低灌注老龄大鼠因大脑血流灌注不足可出现体内胆固醇代谢失衡并出现应激调节现象.%Objective: To investigate the change of cortical blood flow and the dynamic changes of serum total cholesterol(TC ) and high density lipoprotein(HDL) in brains of aging rat during cerebral ischemic injury. Methods:The model of aging rats with cerebral hy-poperfusion was successfully constructed by persistent bilateral common carotid artery ligation(2V0). The cortical blood flow and the concentration of serum TC and HDL at different time points were determined and compared. Results: Compared with the sham-operated group,the temporal blood flow was significantly decreased in 14 d group. But the collateral vessels were gradually regenerated and formed in local brain,while the temporal blood flow was restored in 21 d and 28 d group. The concentration of HDL and TC was significantly higher in 14 d group than in the sham-operated group (P<0.05), and both of them were decreased with the extention of ischemia time. Conclusions:The serum TC and HDL concentration undergo dynamic changes-increasing first and then decreasing during the process

  12. Individual variation in serum cholesterol levels.

    OpenAIRE

    Hegsted, D. M.; Nicolosi, R J

    1987-01-01

    The intraindividual variances in serum/plasma cholesterol levels from a variety of sources have been examined. It is apparent that these are very substantial with mean coefficients of variation usually between 5% and 10%, even when the diet is controlled in metabolic studies. Some subjects show extreme variability from one blood sample to the next. Thus, it is very difficult to assess the degree of risk of individuals according to the guidelines provided by the Consensus Conference on lowerin...

  13. Relationship among levels of serum ferritin, blood lipid and coronary artery stenosis in elderly patients with coronary heart disease%老年冠心病患者血清铁蛋白及血脂水平与冠脉狭窄的相关性

    Institute of Scientific and Technical Information of China (English)

    张晓艳; 刘波; 张伟; 靳英; 李明阳; 魏璇

    2011-01-01

    目的:观察老年冠心病患者血清铁、铁蛋白、转铁蛋白及血脂水平与冠状动脉狭窄程度的关系,探寻冠心病的发病原因.方法:对201例疑似冠心病的老年患者行冠脉造影及血清铁、铁蛋白、转铁蛋白、总胆固醇(TC)、甘油三酯、高密度脂蛋白-胆固醇(HDL-C)及低密度脂蛋白-胆固醇(LDL-C)的测定,采用Gensini评分系统衡量冠状动脉造影结果.按照Gensini评分结果将观察对象分为对照组和冠心病轻、中、重度组,应用方差分析比较各参数在四组中的分布情况,应用多因素回归分析研究Gensini评分与各参数的关系.结果:单因素方差分析显示,血清铁、血清铁蛋白、转铁蛋白/铁蛋白比值、TC及LDL-C在各组间分布差异具有显著性(F=3.519~9.414,P均<0.05);多因素逐步线性回归分析显示,血清铁蛋白与冠状动脉狭窄程度的评分独立相关(偏相关系数=0.182,P<0.05).结论:老年冠心病的发生与血清铁、铁蛋白、总胆固醇和低密度脂蛋白-胆固醇有一定的相关性,血清铁蛋白与冠状动脉狭窄程度呈独立正相关.%Objective: To investigate the relationship among levels of serum iron, ferritin, transferrin (TRF), blood lip-id and coronary artery stenosis in aged patients with coronary heart disease (CHD) as well as try to find the possible cause of CHD. Methods: A total of 201 aged patients suspected CHD underwent coronary angiography and levels of serum iron, ferritin, TRF, total cholesterol (TO, triglyceride (TG), high density lipoprotein cholesterol (HDL-C), low density lipoprotein cholesterol (LDL-C) were examined. Gensini score system was used to evaluate the results of coronary angiography. All patients were allocated into control group, mild, moderate and severe group of CHD according to the Gensini score. The above indexes in the four groups were analyzed and the relationship among serum iron, ferritin, TRF and Gensini score was further

  14. Immunoradiometric assay for ferritin in human serum

    International Nuclear Information System (INIS)

    A sensitiv specific and precise immunoradiometric assay for ferritin has been developed. Ferritin was measured in the serum of 160 hospital controls, 101 females (118 plus/minus 9 μg/l) and 59 males (189 plus/minus 16 μg/l). This difference was statistically significant. In 28 patients with untreated iron deficiency anemia, serum ferritin concentration (6.1plus/minus 0.7 μg/l) was significantly lower than in the controls, but it was within the normal range in 14 cases of polycythaemia vera treated by repeated phlebotomy. In 4 patients with primary haemachromatosis (2884 plus/minus 56 μg/l), 25 with secondary iron overload states (5702 plus/minus 1235 μg/l) and 8 with haemolytic anaemia (1612 plus/minus 605 μg/l), serum ferritin levels were markedly elevated. In 14 cases of transfusional siderosis there was a highly significant correlation between serum ferritin concentration and units of blood transfused. A circadian rhythmin serum ferritin concentration was observed in 7 healthy subjects. (author)

  15. Cord Blood

    Directory of Open Access Journals (Sweden)

    Saeed Abroun

    2014-05-01

    Full Text Available   Stem cells are naïve or master cells. This means they can transform into special 200 cell types as needed by body, and each of these cells has just one function. Stem cells are found in many parts of the human body, although some sources have richer concentrations than others. Some excellent sources of stem cells, such as bone marrow, peripheral blood, cord blood, other tissue stem cells and human embryos, which last one are controversial and their use can be illegal in some countries. Cord blood is a sample of blood taken from a newborn baby's umbilical cord. It is a rich source of stem cells, umbilical cord blood and tissue are collected from material that normally has no use following a child’s birth. Umbilical cord blood and tissue cells are rich sources of stem cells, which have been used in the treatment of over 80 diseases including leukemia, lymphoma and anemia as bone marrow stem cell potency.  The most common disease category has been leukemia. The next largest group is inherited diseases. Patients with lymphoma, myelodysplasia and severe aplastic anemia have also been successfully transplanted with cord blood. Cord blood is obtained by syringing out the placenta through the umbilical cord at the time of childbirth, after the cord has been detached from the newborn. Collecting stem cells from umbilical blood and tissue is ethical, pain-free, safe and simple. When they are needed to treat your child later in life, there will be no rejection or incompatibility issues, as the procedure will be using their own cells. In contrast, stem cells from donors do have these potential problems. By consider about cord blood potency, cord blood banks (familial or public were established. In IRAN, four cord blood banks has activity, Shariati BMT center cord blood bank, Royan familial cord blood banks, Royan public cord blood banks and Iranian Blood Transfusion Organ cord blood banks. Despite 50,000 sample which storage in these banks, but the

  16. Serum bactericidal test.

    OpenAIRE

    Stratton, C W

    1988-01-01

    The serum bactericidal test represents one of the few in vitro tests performed in the clinical microbiology laboratory that combines the interaction of the pathogen, the antimicrobial agent, and the patient. Although the use of such a test antedates the antimicrobial era, its performance, results, and interpretation have been subject to question and controversy. Much of the confusion concerning the serum bactericidal test can be avoided by an understanding of the various factors which influen...

  17. Dosagem de folatos maternos e fetais, séricos e eritrocitários em malformações por defeito de fechamento do tubo neural no feto Maternal and fetal serum and red blood cell folate levels in pregnancies complicated by neural tube defects

    Directory of Open Access Journals (Sweden)

    Victor Bunduki

    1998-07-01

    Full Text Available Objetivos: avaliar os níveis de folatos maternos e fetais gestações com malformações por defeitos de fechamento do tubo neural (DFTN. Métodos: o estudo foi do tipo caso-controle, no qual 14 casos de fetos com DFTN (grupo estudo e 14 casos de fetos com outras malformações (grupo controle foram estudados em gestantes de baixo risco para DFTN. Propusemo-nos a dosar o ácido fólico, na sua forma total e metilada, nos compartimentos fetal e materno, utilizando dosagens séricas e tissulares (eritrocitárias, assim como o volume corpuscular médio, o hematócrito e a hemoglobina. As coletas foram realizadas imediatamente antes da interrupção da gestação. Os resultados nos dois grupos foram comparados pelo teste t de Student, método de amostras pareados pela idade gestacional. Resultados: não se encontrou diferença nas taxas de folatos fetais e nos parâmetros hematológicos dos fetos, entre os dois grupos. Por outro lado, taxas anormalmente baixas de folatos foram encontradas nos eritrócitos das mães portadoras de fetos com DFTN, tanto para as formas totais(293,9 ng/mL contra 399,1 ng/mL no grupo controle, p=0,01 quanto para as formas metiladas (201,9 ng/mL contra 314,0 ng/mL para o grupo controle, p=0,02. Os folatos séricos maternos não se mostraram diferentes nos grupos estudo e controle. Conclusão: este estudo demonstrou que há uma menor taxa de folatos intratissulares, nas mães de fetos acometidos por DFTN, porém com taxas de folatos séricos semelhantes em relação ao grupo controle.Objective: to analyze maternal and fetal folate status in cases of neural tube defects (NTD. Methods: a case-control study was designed with 14 cases of fetuses with neural tube defects (study group and 14 cases of fetuses with other unrelated malformations (control group gestational age matched, in low-risk pregnant women. Both total and methylated folic acid levels in fetal and maternal compartments using serum and tissular (red blood cells

  18. Value of serum marker HE4 in pulmonary carcinoma diagnosis

    OpenAIRE

    Tang, Qin-Fang; Zhou, Zhong-Wei; Ji, Hong-Bing; Pan, Wu-Hua; Sun, Ming-Zhong

    2015-01-01

    An effective blood test is valuable to aid clinicians in making case management decisions. The present study was to analyze the value of four serum tumor markers for the diagnosis of pulmonary carcinoma. The case group consisted of 80 pulmonary carcinoma patients, which were compared to a control group of 30 patients with benign pulmonary disease and a control group of 30 healthy individuals. Serum levels of carcinoma embryonic antigen (CEA), cytokeratin protein fragment 21-1 (CYFRA21-1), neu...

  19. Preanalytical management: serum vacuum tubes validation for routine clinical chemistry

    OpenAIRE

    Lima-Oliveira, Gabriel; Lippi, Giuseppe; Salvagno, Gian Luca; Montagnana, Martina; Picheth, Geraldo; Guidi, Gian Cesare

    2012-01-01

    Introduction: The validation process is essential in accredited clinical laboratories. Aim of this study was to validate five kinds of serum vacuum tubes for routine clinical chemistry laboratory testing. Materials and methods: Blood specimens from 100 volunteers in five different serum vacuum tubes (Tube I: VACUETTE®, Tube II: LABOR IMPORT®, Tube III: S-Monovette®, Tube IV: SST® and Tube V: SST II®) were collected by a single, expert phlebotomist. The routine clinical chemistry tests were...

  20. Elevated Serum Aminotransferases Secondary to Rippling Muscle Disease

    OpenAIRE

    Nalankilli, Kumanan; Lubel, John

    2013-01-01

    A 43-year-old man was referred by his general practitioner to the hepatology clinic with deranged serum aminotransferases, discovered as part of routine blood tests. The objective was to identify the cause of elevated serum aminotransferases in this patient in a systematic manner. Thorough history and physical examination revealed a background history of rippling muscle disease secondary to caveolin-3 protein deficiency, with typical clinical signs. There was a positive family history of musc...

  1. Composição e propriedades físico-químicas de duas frações do soro de sangue bovino Composition and physicochemical properties of two protein fractions of bovine blood serum

    Directory of Open Access Journals (Sweden)

    Ana Silvia Prata

    2008-12-01

    Full Text Available O sangue animal de abatedouros representa uma fonte importante de ingrediente alimentício subutilizado. A produção de sangue bovino no Brasil foi aproximadamente 3,6 x 10(6 tons em 2005. Além do desperdício de uma proteína de boa qualidade, a subutilização deste subproduto da indústria animal é uma fonte importante de poluição dos recursos hídricos e do solo. A concentração de proteína albumina (BSA e globulina (BSG bovinas secas em spray ou liofilizadas foi, aproximadamente, 85,0%. O score de aminoácidos (EAAS foi 72,7%, para BSA liofilizada, e 89,3%, para BSG. As propriedades emulsificantes e espumantes de ambas as frações protéicas foram altas, principalmente a pH 5,5. A capacidade emulsificante e espumante foi maior para BSA, no entanto, tanto a estabilidade da emulsão quanto a da espuma formada foram melhores para a BSG. A adição de NaCl apresentou uma tendência de reduzir as duas propriedades surfactantes, independentemente do pH. Considerando a solubilidade, a estabilidade ao calor, propriedades emulsificante e espumante, tanto a BSA quanto a BSG deveriam ser consideradas bons ingredientes funcionais para a manufatura de produtos alimentícios. O estudo não apenas confirmou dados já reportados na literatura, como também explorou novas propriedades, o que amplifica o potencial de aplicações do soro de sangue bovino.Animal blood from slaughterhouses represent an important source of food ingredients which have been so far underutilized. Bovine blood production in Brazil was about 3.6 x 10(6 in the year 2005. Besides wasting a good food quality protein, underutilization of this byproduct from the animal industry is an important cause of soil and water resources pollution. Protein concentration of both freeze-dried and spray-dried bovine albumin (BSA and globulin (BSG was around 85.0%. Essential Amino Acid Scoring (EAAS was 72.7% for freeze-dried BSA and 89.3% for BSG. Emulsifying and foaming properties of both

  2. Blood / Money

    OpenAIRE

    Strong, Thomas

    1997-01-01

    Marilyn Strathern has argued that "nature" in Euro-American culture has appeared as constraint; it has figured the givens of existence on which human artifice is seen to construct "society" or "culture."(5) Among those givens is the notion that human beings are naturally individuals. And blood, too, images individuality: "The very thought of blood, individual blood, touches the deepest feelings in man about life and death" ([RIchard Titmuss] 16.) Transfusion medicine, then, draws on a series ...

  3. Comparison of different protocols for the bovine viral diarrhea virus detection by RT-PCR in pools of whole blood and blood serum artificially contaminated/ Comparação de diferentes protocolos para a detecção do vírus da diarréia viral bovina por RT-PCR em grupos de sangue total e de soro sangüíneo, artificialmente contaminados

    Directory of Open Access Journals (Sweden)

    Amauri A. Alfieri

    2005-06-01

    Full Text Available The RT-PCR technique was optimized and evaluated to detect the 5’ untranslated region of bovine viral diarrhea virus (BVDV from clinical samples that consisted of blood serum and whole blood artificially contaminated with the NADL strain of BVDV. To optimization of technique, the following conditions were evaluated: i two pairs of primers, 103 / 372 (WEINSTOCK et al., 2001 and 324 / 326 (VILCEK et al., 1994, ii four methods of nucleic acid extraction (phenol/chloroform/isoamyl alcohol; silica/guanidine isothiocyanate; a combination of the two previous methods; and TRIzol™ and iii different concentrations and compositions of reagents and time/temperature of the reactions. Between the alternatives tested that resulted in the amplification of the 290 bp product that was easily visualized in ethidium bromide stained 2% agarose gel was that presented the following conditions: i primers 103 and 372; ii initial volume and clinical sample: 50 mL of blood serum; iii extraction of nucleic acid: silica/guanidine isothiocyanate method; iv reverse transcription: 9 mL extracted nucleic acid, 1xPCR buffer (20 mM Tris-HCl pH 8.4 and 50 mM KCl, 1.5 mM MgCl2; 60 units of reverse transcriptase enzyme M-MLV, RNA denaturation at 97°C / 4 min, and reverse transcription at 42°C / 30 min; v PCR: primers 103 / 372 with anneling temperature at 59°C. The utilization of RT-PCR within these optimized conditions allowed the amplification of the BVDV NADL strain (103,56 TCID50 from pools of artificially contaminated blood serum until the dilution 1:160.A técnica da RT-PCR foi otimizada e avaliada para a detecção da região 5’ terminal não-traduzida do genoma do vírus da diarréia viral bovina (BVDV em amostras clínicas de bovinos, constituídas por soro sangüíneo e sangue total, artificialmente contaminadas com a estirpe NADL do BVDV. Para a otimização da técnica foram avaliados: i dois pares de primers, 103 / 372 (WEINSTOCK et al., 2001 e 324 / 326

  4. Ion suppression from blood collection devices

    DEFF Research Database (Denmark)

    Hasselstrøm, Jørgen Bo; Sejr Gothelf, Aase

    The aim of the study was to examine the variation in ion suppression in ultra high pressure liquid chromatography tandem mass spectrometry (UHPLC-MS-MS) methods when using different blood collection devices. Three different methods measuring 18 antidepressants and antipsychotics in total were...... studied. The blood collection devices were all designed to activate clot formation. They were made of glass with or without silicone coating or plastic containing silicate particles, thrombin or polystyrene particles coated with kaolin. The blood collection devises Venoject and Venosafe were supplied from...... Terumo, S-monovette from Sarstedt, Vacuette from Greiner Bio-One and three BD Vacutainer serum tubes from BD. These seven different blood collection devices were used to withdraw blood from five healthy drug free donors (n=35) in random order. The samples were centrifuged and serum from each sample...

  5. TCM syndrome type distribution and its correlations with blood lipids, hepato-enzymes, serum leptin and insulin resistance in patients with non-alcoholic fatty liver disease%NAFLD患者中医证候类型与血脂、肝功能酶学、血清瘦素及胰岛素抵抗指数的关系

    Institute of Scientific and Technical Information of China (English)

    周晓玲; 谢胜; 李灿; 侯秋科; 罗婷; 王金国

    2012-01-01

    Objective It is to investigate the distribution of TCM syndromes of non-alcoholic fatty liver disease ( NAFLD ) and its correlations with blood lipids, hepato-enzymes, serum leptin and insulin resistance ( IR ) to guild better for the syndrome differentiation and treatment of this disease. Methods The syndromes of 576 NAFLD patients were categorized, and its correlations with blood lipids, hepato-enzymes, serum leptin and IR were analyzed. Results Among the 576 patients, 202 were categorized as Yang-deficiency of both spleen and kidney type, and 218 as stagnation of liver Qi and spleen deficiency type, accounting for 72. 9% . Levels of blood lipids ( TC , TG, LDL ) , hepato-enzymes ( ALT, AST, GGT ), leptin and IR in patients of Yang-deficiency of both spleen and kidney type were higher than those in patients of other types, HDL were lower than those in patients of other types, the differences were statistically significant. Conclusion Yang-deficiency of both spleen and kidney type and stagnation of liver Qi and spleen deficiency type are the dominant syndromes in patients with NAFLD in Guangxi province; abnormal blood lipids, hepato-enzymes, leptin and IR may present in those of Yang-deficiency of both spleen and kidney type more frequently. Yang-deficiency of spleen is the key pathology of NAFLD.%目的 探讨非酒精性脂肪性肝病(NAFLD)中医证候类型及其与血脂、肝功能酶学、血清瘦素以及胰岛素抵抗指数等的关系,以更好地指导NAFLD的辨证论治.方法 对诊断为NAFLD的576例患者进行中医证候分型,并观察其证型与血脂、肝功能酶学、血清瘦素及胰岛素抵抗指数的关系.结果 576例NAFLD患者中,脾肾阳虚证202例,肝郁脾虚证218例,占全部病例的72.9%.脾肾阳虚证患者的血脂(TC、TG、LDL)、肝功能酶学(ALT、AST、GGT)水平、血清瘦素以及胰岛素抵抗指数较其他证候类型患者明显增高,HDL则明显低于其他证候类型.结论 脾肾阳虚证和

  6. 西洛他唑对老年急性冠脉综合征患者血清炎性细胞因子及血脂代谢的影响%Effects of cilostazol on the serum levels of inflammatory factors and blood lipid in elderly patients with acute coronary syndrome

    Institute of Scientific and Technical Information of China (English)

    刘永升; 魏梅; 刘会英; 岳媛媛; 王乐; 张慧敏; 刘丽

    2013-01-01

    目的 探讨西洛他唑对老年急性冠脉综合征患者血清中炎性细胞因子及血脂的影响. 方法 选择老年急性冠脉综合征患者60例,随机分为基本治疗组和西洛他唑组,同时以30例健康体检人员作为正常对照组.基本治疗组给予常规药物和阿托伐他汀治疗,西洛他唑组在基本治疗组所用药物外,增加西洛他唑.治疗6周后观察各组血清中白介素-1β、超敏C反应蛋白、基质金属蛋白酶-9及血脂的变化. 结果 2组治疗后白介素-1β、超敏C反应蛋白、基质金属蛋白酶-9水平较治疗前均有下降(P<0.05),但西洛他唑组下降幅度大于基本治疗组(P<0.05).2组治疗后血脂水平均较前改善,且西洛他唑组改善较基本治疗组明显(P<0.05). 结论 西洛他唑具有降低炎性因子浓度、调节血脂代谢的作用,对于老年急性冠脉综合征患者的治疗具有积极意义.%Objective To observe the effects of cilostazol on the serum level of interleukin-1β(IL-1β),high-sensitive C reactive protein (hs-CRP),blood lipid and matrix metalloproteinase-9 (MMP-9) in the elderly patients with acute coronary syndrome (ACS).Methods Sixty elderly patients with ACS were randomly assigned to conventional therapy group and cilostazol group.Thirty healthy people were selected as the normal group.The patients in conventional therapy group were given routine treatment.The patients in cilostazol group were given cilostazol besides routine treatment.The serum levels of IL-1β,hs-CRP,blood lipid and MMP-9 were measured before and 6 weeks after therapy.Results The levels of IL-1β,hs-CRP and MMP-9 were significantly decreased in both groups (P < 0.05),especially in cilostazol group (P < 0.05).The level of blood lipid was also significantly improved in both group(P < 0.05),and the improvement was more significant in cilostazol group (P < 0.05).Conclusions The therapy of cilostazol can adjust blood lipid metabolism and decrease the

  7. Understanding Blood Counts

    Science.gov (United States)

    ... Lab and Imaging Tests Understanding Blood Counts Understanding Blood Counts Understanding Blood Counts SHARE: Print Glossary Blood cell counts give ... your blood that's occupied by red cells. Normal Blood Counts Normal blood counts fall within a range ...

  8. Hematology and serum chemistry of cottontail rabbits of southern Illinois.

    Science.gov (United States)

    Lepitzki, D A; Woolf, A

    1991-10-01

    In 1983 and 1984 blood was collected from 79 cottontail rabbits (Sylvilagus floridanus) confined to an outdoor enclosure in southern Illinois to establish reference values for hematology and serum chemistry. Packed cell volume, sodium, potassium, chloride, glucose, calcium, carbon dioxide, blood urea nitrogen, creatinine, uric acid, cholesterol, albumin, bilirubin, alkaline phosphatase, aspartate transaminase, alanine aminotransaminase, total protein, albumin/globulin ratio, and osmolality were measured. Sex and age (adult versus juvenile) of rabbit as well as season (June to September versus October to May) and method of capture (trap versus shot) variously affected most hematology and serum chemistry variables. PMID:1758030

  9. Blood donation

    CERN Multimedia

    GS Department

    2009-01-01

    A blood donation is organised by the Cantonal Hospital of Geneva On Thursday 19 March 2009 from 9 a.m. to 5 p.m. CERN RESTAURANT 2 Number of donations during the last blood donations :135 donors in July 2008 122 donors in November 2008 Let’s do better in 2009 !!! Give 30 minutes of your time to save lives...

  10. BLOOD DONATION

    CERN Multimedia

    SC Unit

    2008-01-01

    A blood donation, organized by EFS (Etablissement Français du Sang) of Annemasse will take place On Wednesday 12 November 2008, from 8:30 to 16:00, at CERN Restaurant 2 If possible, please, bring your blood group Card.

  11. Effects of coagulation temperature on measurements of complement function in serum samples from patients with systemic lupus erythematosus.

    OpenAIRE

    Baatrup, G; Sturfelt, G; Junker, A; Svehag, S E

    1992-01-01

    Blood samples from 15 patients with systemic lupus erythematosus (SLE) and 15 healthy blood donors were allowed to coagulate for one hour at room temperature, followed by one hour at 4 or 37 degrees C. The complement activity of the serum samples was assessed by three different functional assays. Serum samples from patients with SLE obtained by coagulation at 37 degrees C had a lower complement activity than serum samples from blood coagulated at 4 degrees C when the capacity of the serum sam...

  12. Pediatric patient with Bombay blood group: A rare case report

    Directory of Open Access Journals (Sweden)

    Sudeshna Bhar (Kundu

    2015-01-01

    Full Text Available Bombay blood group is a rare blood group in which there is the absence of H antigen and presence of anti-H antibodies. At the time of blood grouping, this blood group mimics O blood group due to the absence of H antigen, but it shows incompatibility with O group blood during cross matching. Serum grouping or reverse grouping are essential for confirmation of the diagnosis. Patients carrying this blood group can receive blood only from a person with this blood group. Reported cases of anesthesia in the pediatric patient with Bombay blood group are relatively rare. Here, we present successful anesthetic management along with intraoperative blood transfusion in a pediatric patient with Bombay blood group posted for ovarian cystectomy.

  13. Pediatric patient with Bombay blood group: A rare case report.

    Science.gov (United States)

    Bhar Kundu, Sudeshna; De, Anisha; Saha, Anindita; Bhattacharyya, Chiranjib

    2015-01-01

    Bombay blood group is a rare blood group in which there is the absence of H antigen and presence of anti-H antibodies. At the time of blood grouping, this blood group mimics O blood group due to the absence of H antigen, but it shows incompatibility with O group blood during cross matching. Serum grouping or reverse grouping are essential for confirmation of the diagnosis. Patients carrying this blood group can receive blood only from a person with this blood group. Reported cases of anesthesia in the pediatric patient with Bombay blood group are relatively rare. Here, we present successful anesthetic management along with intraoperative blood transfusion in a pediatric patient with Bombay blood group posted for ovarian cystectomy. PMID:26240554

  14. Influence of serum resistin level on blood pressure in patients with type 2 diabetes mellitus complicated hypertension%血清抵抗素水平对2型糖尿病合并高血压患者血压的影响

    Institute of Scientific and Technical Information of China (English)

    李玉; 段敬柱; 王崇全; 陈相健

    2015-01-01

    目的:研究血清抵抗素(SR)水平与2型糖尿病(T2DM)合并高血压患者血压的相关性。方法:选择T2DM合并高血压患者(T2DM合并高血压组)124例,单纯T2DM患者(单纯糖尿病组)85例,正常对象(正常对照组)71例纳入研究。根据血压水平,T2DM合并高血压组被进一步分为1级亚组(34例),2级亚组(43例)和3级亚组(47例)。取外周静脉血测量三组SR及其他指标水平。Logistic多元回归分析用来分析影响血压水平的危险因素。Kendall′s tau‐b相关分析用来分析SR ,空腹胰岛素,量化胰岛素敏感指数和胰岛素抵抗指数与血压水平的相关性。结果:与健康对照组比较,单纯糖尿病组和T2DM合并高血压组SR水平显著升高[(7.21±2.65) ng/ml比(19.87±3.67) ng/ml比(31.32±3.89) ng/ml],且T2DM合并高血压组的显著高于单纯糖尿病组的(P均<0.01)。Logistic多元回归分析表明SR水平是影响患者血压水平的独立危险因素(R2=0.087, P<0.05)。Kendall′s tau‐b相关分析显示SR水平与高血压患者血压水平呈显著正相关( r=0.202, P=0.001);控制其余影响因素后,二者间仍然呈显著正相关( r=0.233, P=0.022)。结论:血清抵抗素水平是2型糖尿病合并高血压患者的危险因素,与其血压水平呈显著正相关。%Objective:To study the correlation between serum resistin (SR) level and blood pressure in patients with type 2 diabetes mellitus (T2DM) complicated hypertension .Methods :A total of 124 patients with T2DM complicat‐ed hypertension (T2DM + hypertension group) ,85 patients with pure T2DM (pure DM group) and 71 normal sub‐jects (normal control group) were enrolled .According to blood pressure level ,T2DM + hypertension group was further divided into stage 1 subgroup (n=34) ,stage 2 subgroup (n=43) and stage 3 subgroup (n=47) .Peripheral venous blood was taken

  15. Tainted blood

    DEFF Research Database (Denmark)

    Deleuran, Ida; Sheikh, Zainab Afshan; Hoeyer, Klaus

    2015-01-01

    study of the historical rise and current workings of safety practices in the Danish blood system. Here, we identify a strong focus on contamination in order to avoid 'tainted blood', at the expense of working with risks that could be avoided through enhanced blood monitoring practices. Of further...... significance to this focus are the social dynamics found at the heart of safety practices aimed at avoiding contamination. We argue that such dynamics need more attention, in order to achieve good health outcomes in transfusion medicine. Thus, we conclude that, to ensure continuously safe blood systems, we...... need to move beyond the bifurcation of the social and medical aspects of blood supply as two separate issues and approach social dynamics as key medical safety questions....

  16. Relationship of serum retinol-binding protein 4 with blood lipid and cystatin C in patients with newly-diagnosed type 2 diabetes mellitus%初诊2型糖尿病患者视黄醇结合蛋白4与血脂及胱仰素C的关系

    Institute of Scientific and Technical Information of China (English)

    王晶; 李强; 陈迪群; 张希尧; 韩玉冰; 王敏楠; 王薇; 张巾超

    2013-01-01

    目的 探讨初诊2型糖尿病患者血清视黄醇结合蛋白(RBP4)与血脂及胱抑素C的关系.方法 选取研究对象152例,其中新诊断2型糖尿病伴血脂异常组39例,新诊断2型糖尿病血脂正常组39例;无2型糖尿病血脂异常组36例,无2型糖尿病血脂正常组38例.测定受试者的体重指数(BMI)、腰臀比、血压、机体脂肪百分比(F%),检测4组空腹血清RBP4、血脂和胱抑素C水平,对血清RBP4、血脂及胱抑素C进行单因素相关分析,然后进行多元逐步回归分析.结果 糖尿病血脂正常组的RBP4水平高于无糖尿病血脂正常组(P<0.05),糖尿病血脂异常组的RBP4水平高于无糖尿病血脂异常组(P<0.05),单因素相关分析显示,RBP4与总胆固醇(TC)、甘油三酯(TG)、空腹血糖(FPG)、载脂蛋白(Apo)A、ApoB、低密度脂蛋白胆固醇(LDL-C)、BMI和F%呈正相关(均P<0.01);RBP4与高密度脂蛋白胆固醇(HDL-C)、ApoA、ApoA/ApoB比值呈负相关(均P<0.05).多元逐步回归分析显示,ApoA、ApoB、ApoA/ApoB比值、BMI和TC是RBP4的影响因素,RBP4与ApoA、ApoA/ApoB比值呈负相关,RBP4与ApoB、BMI和TC呈正相关(均P<0.05).RBP4与胱抑素C无相关性.结论 在初诊2型糖尿病患者中,RBP4与ApoA、ApoB相关,而与胱抑素C无关.%Objective To explore the relationship of serum retinol-binding protein 4 (RBP4) with cystatin-C and blood lipid in patients with newly-diagnosed type 2 diabetes mellitus (T2DM).Methods A total of 152 subjects were investigated,including 78 patients with newly-diagnosed T2DM (39 with dyslipidemia and 39 with normal lipidemia) and 74 control subjects (36 with dyslipidemia and 38 with normal lipidemia).The anthropometrical parameters such as body mass index (BMI),percentage of body fat (F%),blood pressure,and waist hip ratio (WHR) were evaluated.Fasting serum RBP4,blood lipid,and cystatin-C levels were determined.Univariate analysis was performed for RBP4,blood lipid,and cystatin

  17. Ferritin in cattle serum

    International Nuclear Information System (INIS)

    A commercially available radioimmunoassay kit for human serum ferritin was used to determine the ferritin concentration in serum or plasma of 41 calves from 0 to 106 days of age, 192 cows and 35 bulls from 2 to 11 years of age. The geometric average concentration of ferritin was 2.1, 12.6 and 4.5 ng/ml for the calves, cows and bulls, respectively. The cows were statistically different from the calves and bulls; there were no differences between the calves and bulls. Within the cows one herd was found to have lower serum ferritin levels than all the other herds (P < 0.05) but no differences in packed cell volumes were present. The data suggest that a radioimmunoassay procedure with a ferritin antibody specific for bovine ferritin could be useful in the study of iron metabolism in cattle. (author)

  18. 群体反应性抗体干扰脐血CD34+细胞增殖、分化的实验研究%Effect of the serum panel reactive antibody on proliferation and differentiation of cord blood CD34+cells in vitro

    Institute of Scientific and Technical Information of China (English)

    杨兴鸽; 方建培; 吴燕峰; 翁文骏; 徐宏贵; 许吕宏

    2008-01-01

    Objective The low rate of engraftment in children with β-thalassemla has seriouslyrestricted the popularity of the hematopoietic stem cell transplantation (HSCT). Panel reactive antibody(PRA) has been regarded as one of the important factors for the success of kidney transplantation. Poly-transfusion before transplantation is associated with the production of PRA. Also PRA is produced in thechildren with β-thalassemia major who need poly-transfusion for life. PRA might be one of factors inducingthe low rate of engraftment in children with β-thalassemia. This study focused on observing the effect of PRAon the proliferation, differentiation, apoptosis and necrosis of cord blood CD34+ cells in vitro by incubatingthe cord blood CD34+ cells with serum containing PRA. Method Seven samples of cord blood werecollected and the HLA typing for every sample was done. Seven sera positive for PRA and seven negativesera were selected respectively. Mononuclear cells ( MNCs ) were obtained by FicoU-Hapaque densitygradient centrifugation. CD34+ cells were isolated from MNCs by positive selection using an immunomagneticseparation ( CD34+ progenitor cell isolation kit and auto-MACS). The CD34+ ceils of umbilical cord bloodwere incubated with the serum and complement in the following groups: A (absence of serum), B (presenceof PRA positive serum ), C (presence of PRA positive serum and complement ), D (presence ofcomplement), and E ( presence of PRA negative serum). After incubation the samples were centrifuged andthe supernatant was collected for LDH detection. At the same time the CD34+ cells were harvested forassessing the expression of Annexin V and CD95 of the CD34+ cells by flow cytometry and also for thedetection of the DNA synthesis by3H-TaR incorporation. Meanwhile the cells were inoculated into themethylcellulose cultural system. The proliferation and hematopoietic potential of the CD34 + cell of cord bloodby the colony formation assay were detected on the day 10. Result

  19. Alterations of serum cholesterol and serum lipoprotein in breast cancer of women

    OpenAIRE

    Hasija, Kiran; Bagga, Hardeep K.

    2005-01-01

    Fasting blood sample of 50 normal subjects (control) and 100 patients of breast cancer were investigated for serum total cholesterol, high density lipoprotein cholesterol, low density lipoprotein cholesterol, very low density lipoprotein, high density lipoprotein cholesterol:low density lipoprotein cholesterol ratio and total cholesterol:high density lipoprotein cholesterol ratio during breast cancer of women. Five cancer stages, types, age groups, parity and menopausal status were undertaken...

  20. Serum magnesium and irradiation

    International Nuclear Information System (INIS)

    Serum magnesium determinations were obtained on 10 dogs and 11 patients undergoing fractionated irradiation to the pelvis and lower abdomen. Five of the dogs received oral prednisone during irradiation. There was no significant change in magnesium concentration in either the control dogs or the patients, but there was a significant increase in stool frequency in both the dogs and patients. A significant increase in magnesium concentration was noted in the dogs receiving prednisone. It is concluded that radiation-induced diarrhea is not caused by reduced serum magnesium concentration

  1. Serum ferritin level in patients with type-2 diabetes mellitus

    OpenAIRE

    Raghavani, Pratik Hasmukhrai; Sirajwala, Habibunnisha B.

    2014-01-01

    Aim: To measure the level of Serum Ferritin, Fasting and Postprandial Blood Sugar & Lipid Profiles in patient with Type-2 Diabetes Mellitus with and without cardiovascular involvement.Material and methods: 50 patients of Type-2 Diabetes Mellitus (DM) patients without any cardiovascular involvement, 50 patients of Type-2 Diabetes Mellitus patients with cardiovascular involvement and 50 age and sex matched normal healthy control were studied. Blood samples were analyzed for ferritin estimat...

  2. Radioimmunoassay of haloperidol in human serum: correlation of serum haloperidol with serum prolactin

    International Nuclear Information System (INIS)

    A radioimmunoassay (RIA) for measurement of serum haloperidol is described. Compared to gaschromatography (GC), RIA vaues average 40% higher. However, a simple organic extraction of serum yields statistically equivalent RIA and GC haloperidol determinations. For both men and women combined, there was a positive correlation between dose (mg/kg/day) and steady-state serum haloperidol level (r = +0.86) and between steady-state serum haloperidol and serum prolactin (PRL) concentration

  3. Relación entre residuos de clorpirifos en leche y sangre de vacas Holstein y niveles séricos de estradiol y tiroxina - Chlorpyrifos residues in milk and blood in Holstein cows and their relation to estradiol and thyroxin serum levels

    Directory of Open Access Journals (Sweden)

    Morales Vallecilla, Carlos

    2010-01-01

    to control grass insect pests in the dairy regions of Antioquia, Colombia, has been linkedto thyroid hormone and estradiol alterations. This study used gaschromatography to detect chlorpyrifos residues in the milk and blood of Holstein cows, fed with chlorpyrifos treated grass. Additionally, it measured, by radioimmunoanalysis, the blood levels of thyroid hormone and estradiol in the cows the day before their entrance in the treated pastures and through-out the following 30 days. Although gas chromatography was effective in detecting chlorpyrifos in milk and serum during standardization, it did not detect any chlorpyrifos residues in the serum and milk samples from the studied animals.No significant difference was found in thyroid hormone and estradiol levels through-out the treatment. These findings indicate that treating grass with chlorpyrifos according to manufacturer’s instructions does not generate detectable residues of the insecticide in the blood and milk of the cows feeding on the grass. The results could also indicate that under the manufacturer’s recommendations, chlorpyrifos is not and endocrine disruptor for dairy cows.

  4. Occult HCV in Egyptian volunteer blood donors

    Directory of Open Access Journals (Sweden)

    Mostafa A. Amin 1, Kouka S. E. Abdel-Wahab 2 and Adel A.

    2013-01-01

    Full Text Available Objective: The study aims to investigate the risk of post-transfusion transmission of hepatitis c virus (HCV in the circumstances of occult HCV when anti-HCV is undetectable by ELISA and HCV-RNA is detected by RT-PCR in the plasma and or in peripheral blood mononuclear cells (PBMCs of donor blood and the recipients are immunocompromised. Patients & methods: The study covered 18 chronic renal failure patients (CRF [12 males (66.7% their age ranged from 28 to 65 years and 6 females (33.3 % their age ranged from 15 to 55 years] undergoing hemodialysis in Nile Hospital as part of their therapy have to receive blood transfusions (275 blood units for the first time. Commercial ELISA kits for anti-HCV and nested-RT-PCR (N-RT-PCR kits were used. Results: Anti-HCV was positive in one serum from the eighteen (5.5% poly transfused CRF patients at the end of the study while the seventeen sera were negative. This serum was also positive for HCV RNA by N-RT-PCR. Out of the 20 transfused blood units, one blood unit (three components were tested by blood banking anti-HCV negative by ELISA, were positive for HCV RNA by N-RT-PCR. The collective markers of this blood unit represent an occult HCV. The risk of acquiring post-transfusion HCV infection from an occult HCV blood unit is 5%. Real time PCR showed variation in the viral load of the serum of the infected CRF patient, the plasma of blood unit, the PBMCs of this blood unit whether activated by PHA-M or not.

  5. Donating Blood

    Science.gov (United States)

    ... And be sure to drink plenty of water, milk, or other liquids. Before donating, you'll need to answer some questions about your medical history, and have your temperature, pulse, blood pressure, and ...

  6. Blood smear

    Science.gov (United States)

    ... of RBCs due to body destroying them ( immune hemolytic anemia ) Low number of RBCs due to some red ... of Heinz bodies may indicate: Alpha thalassemia Congenital hemolytic anemia Disorder in which red blood cells break down ...

  7. Amylase - blood

    Science.gov (United States)

    Amylase is an enzyme that helps digest carbohydrates. It is made in the pancreas and the glands ... saliva. When the pancreas is diseased or inflamed, amylase releases into the blood. A test can be ...

  8. Postmortem blood ferritin concentrations in sudden infant death syndrome.

    OpenAIRE

    Worwood, M.; Raha-Chowdhury, R.; Fagan, D G; Moore, C.A.

    1995-01-01

    AIMS--To confirm the observation of extremely high concentrations of ferritin in postmortem serum samples in sudden infant death syndrome (SIDS); to examine the factors influencing blood ferritin concentrations postmortem; to determine whether or not these high blood ferritin concentrations are characteristic of SIDS. METHODS--Postmortem samples of cardiac blood were obtained from 58 full term infants who died of SIDS and 14 full-term infants who died of a variety of other causes. Whole blood...

  9. Moving blood.

    Science.gov (United States)

    Pelis, K

    1997-01-01

    Our internationally acclaimed journalist Sanguinia has returned safely from her historic assignment. Travelling from Homeric Greece to British Romanticism, she was witness to blood drinking, letting, bathing, and transfusion. In this report, she explores connections between the symbolic and the sadistic; the mythic and the medical--all in an effort to appreciate the layered meanings our culture has given to the movement of blood between our bodies. PMID:9407636

  10. Determination Melatonin in Serum of Kurdish Horses by HPLC in Kermanshah Region at Breeding Season

    Directory of Open Access Journals (Sweden)

    Afsaneh Arabi

    2012-06-01

    Full Text Available The objective of this study was to evaluate changes in serum concentration of melatonin of Kurdish horses Kermanshah region at breeding season (February to June of 2010. Blood samples from jugular vein of 40 Kurdish horses were collected. Serum was harvested from these blood samples by centrifugation. After preparation and derivation of serums for HPLC assay, samples were injected to HPLC column and melatonin was measured by area under curve based on standard curve of melatonin. The mean (±S.E of serum melatonin was determined 63.23±9.51 pg/mL. The concentration of serum melatonin of mares was nearly 2 times than stallions and significantly differed (p = 0.01. Thus serum melatonin related to breed of horses and may affect reproductive activity in different breed and geographical region.

  11. Use of In Vitro Assays To Determine Effects of Human Serum on Biological Characteristics of Acanthamoeba castellanii

    OpenAIRE

    Sissons, James; Alsam, Selwa; Stins, Monique; Rivas, Antonio Ortega; Morales, Jacob Lorenzo; Faull, Jane; Khan, Naveed Ahmed

    2006-01-01

    Normal human serum inhibits Acanthamoeba (encephalitis isolate) binding to and cytotoxicity of human brain microvascular endothelial cells, which constitute the blood-brain barrier. Zymographic assays revealed that serum inhibits extracellular protease activities of acanthamoebae. But it is most likely that inhibition of specific properties of acanthamoebae is a consequence of the initial amoebicidal-amoebistatic effects induced by serum. For example, serum exhibited amoebicidal effects; i.e....

  12. Blood Clotting and Pregnancy

    Medline Plus

    Full Text Available ... Blood Basics Blood Disorders Anemia Bleeding Disorders Blood Cancers Blood Clots Blood Clotting and Pregnancy Clots and ... Increased maternal age Other medical illness (e.g., cancer, infection) back to top How are Blood Clots ...

  13. Biology of Blood

    Science.gov (United States)

    ... Mail Facebook TwitterTitle Google+ LinkedIn Home Blood Disorders Biology of Blood Overview of Blood Medical Dictionary Also ... Version. DOCTORS: Click here for the Professional Version Biology of Blood Overview of Blood Components of Blood ...

  14. Blood (For Parents)

    Science.gov (United States)

    ... Story" 5 Things to Know About Zika & Pregnancy Blood KidsHealth > For Parents > Blood Print A A A ... about the mysterious, life-sustaining fluid called blood. Blood Basics Two types of blood vessels carry blood ...

  15. Blood Facts and Statistics

    Science.gov (United States)

    ... About Blood > Blood Facts and Statistics Printable Version Blood Facts and Statistics Facts about blood needs Facts ... about American Red Cross Blood Services Facts about blood needs Every two seconds someone in the U.S. ...

  16. Catecholamine blood test

    Science.gov (United States)

    Norepinephrine -- blood; Epinephrine -- blood; Adrenalin -- blood; Dopamine -- blood ... A blood sample is needed. ... the test. This is especially true if both blood and urine catecholamines are to be measured. You ...

  17. Utilización del Método de Elisa en la detección directa de antígeno de virus diarrea viral bovina en muestras de suero sanguíneo de bovinos Use of an ELISA test in the direct diagnosis of viral antigens of Bovine Viral Diarrhea Virus (BVDV in bovine blood serum samples

    Directory of Open Access Journals (Sweden)

    G. REINHARDT

    2003-01-01

    Full Text Available El virus de la Diarrea Viral Bovina (VDVB es un agente infeccioso importante del ganado bovino y está distribuido ampliamente en el mundo, produciendo pérdidas económicas sustanciales en la producción pecuaria. La principal fuente de contagio de los animales susceptibles está en las secreciones y excreciones de los animales infectados persistentes e inmunotolerantes (PI, condición que se produce en la etapa gestacional, específicamente antes de los 120 días de preñez, período en que el sistema inmune del embrión aún no se desarrolla adecuadamente. El propósito de este estudio fue aplicar la utilización de un método inmunoenzimático (ELISA-antígeno para detectar la presencia de animales PI en planteles lecheros de la Xª Región de Chile, a partir de muestras de suero sanguíneo. Para ello se examinaron 335 sueros de bovinos provenientes de 9 predios. Los resultados obtenidos indican que el 33.3% de los planteles analizados presentaron algún animal PI y que a nivel de prevalencia intrapredial, ella varió entre 0.7 y 1.0%. Se concluyó que el método utilizado permite detectar animales PI en forma rápida y sencilla, pudiendo utilizarse en gran cantidad de muestrasBVDV is an important virus of cattle worldwide that induces to substantial economic losses in dairy farms. The major source of infection are secretions and excretions of immunotolerant and persistent infected cattle. That condition is adquired during the early gestational period. The scope of this communication is to inform the use of an ELISA test to detect BVDV persistent infected bovine using blood serum samples in cattle of 9 dairy farms from the Xth. Region of Chile. The results indicated that 0.3% of the serum samples were positive to the ELISA test, and 33.3% of the dairy herds with persistently infected animals. It is concluded that this method diagnose persistently infected cattle, and is very easy to manipulate therefore, is possible to test many animals in

  18. The Levels of Serum Nitric Oxide and Blood Rheology in Patients with Chronic Obstructive Pulmona-ry Disease%慢性阻塞性肺疾病患者血清一氧化氮水平和血液流变性变化

    Institute of Scientific and Technical Information of China (English)

    田强; 沈云峰; 张洪波

    2016-01-01

    Objective:To analyze the changes of serum nitric oxide (NO) and blood rheology indices in pa‐tients with chronic obstructive pulmonary disease (COPD) and the correlation between the indices and the stages of COPD .Method:182 cases of COPD were selected as COPD group ,including 88 cases with acute exacerbation stage of COPD (AECOPD) and 94 cases with stable stage of COPD ,92 cases of health physical examination persons were selected as control group at the same period .All cases were tested serum NO ,PaO2 and the indices of blood rheolo‐gy ,including high shear rate (200s-1 ) and low shear rate (3s-1 ) apparent viscosity ,erythrocyte aggregation index , erythrocyte rigidity index and plasma viscosity (200s-1 ) .The differences between the groups and the correlation with COPD stages were analyzed .Results:The levels of NO and PaO2 in COPD group were significantly lower than that in control group (P<0 .01) ,and that in AECOPD patients were lower than the stable COPD patients (P<0 . 01) .The indices of blood rheology in COPD group were significantly higher than that in control group (P<0 .01) , and AECOPD patients were higher than the stable COPD patients (P<0 .01) .The levels of NO and PaO2 had re‐spectively significant negative relationship with the stages of COPD (r= -0 .641 ,-0 .786 ,P<0 .05) ,the levels of high shear rate and low shear rate apparent viscosity ,erythrocyte aggregation index ,erythrocyte rigidity index and plasma viscosity had respectively significant positive relationship with the stages of COPD (r= 0 .563 ,0 .572 ,0 .643 ,0 .485 ,0 .526 ,P<0 .05) .Conclusion:The decreased serum NO and abnormal blood rheology indices are the reasons for COPD exacerbations ,and targeted therapy can benefit patients .%目的:检测分析慢性阻塞性肺疾病(COPD)患者血清一氧化氮(NO)水平和血液流变学指标变化及与病程分期的相关性。方法:确诊COPD患者182例(COPD组),其中急性加重期88例,稳定期94

  19. Impacts on Serum Leptin for the Patients with Polycystic Ovary Syndrome Treated with the Therapy for Tonifying the Kidney,Activating Blood Circulation and Resolving Phlegm%补肾活血化痰法对多囊卵巢综合征患者血清瘦素的影响

    Institute of Scientific and Technical Information of China (English)

    逯克娜; 林寒梅; 白华

    2012-01-01

    目的 观察补肾活血化痰法组方中药对多囊卵巢综合征(PCOS)患者血清瘦素(Leptin)水平变化的影响.方法 将90例PCOS患者随机分为三组,中药治疗组30例,采用补肾活血化痰法中药治疗;中西药治疗组30例,采用补肾活血化痰法中药+西药(达英-35+二甲双胍)治疗,对照组30例,采用达英-35+二甲双胍治疗,连续治疗3个月经周期.检测三组患者治疗前后血清中Leptin浓度以及卵泡刺激素(FSH)、黄体生成素(LH)、睾酮(T)数值,并进行比较.结果 治疗后三组LH 、LH/FSH 、T、Leptin水平均较治疗前降低,治疗前后组内比较差异有统计学意义(P<0.05);中药治疗组与西药对照组比较,对以上四组数值的影响并无明显优势(P>0.05),而中西医治疗组与西药对照组比较,虽然LH 、LH/FSH的治疗前后组间比较差异无统计学意义,但中西医治疗组T、Leptin水平比西药治疗组变化更为显著,组间比较差异有统计学意义(P<0.05).结论 补肾活血化痰中药联合达英-35、二甲双胍能有效降低PCOS患者的血清Leptin水平,治疗效果优于单纯使用中药或西药.%Objective To observe the impacts of serum leptin for the patients with polycystic ovary syndrome( PCOS )treated with the herbal recipe for tonifying the kidney, activating blood circulation and resolving phlegm. Method 90 cases of PCOS were randomized into three groups. In a Chinese medicine treatment group( 30 cases ), Chinese herbal therapy was adopted for tonifying the kidney, activating blood circulation and resolving phlegm. In an integrated Chinese and western medicine treatment group( 30 cases ), Chinese herbal therapy( tonifying the kidney, activating blood circulation and resolving phlegm ) + western medi-cine( Diane - 35 + metformin )was adopted. In a control group( 30 cases ), Diane-35 + metformin was a-dopted. The treatment lasted continuously for 3 menstrual cycles. The values of leptin concentration, follicle

  20. Serum Biochemistry of Lumpy Skin Disease Virus-Infected Cattle

    Science.gov (United States)

    Avci, Oğuzhan; Doğan, Müge; İnce, Ömer Barış

    2016-01-01

    Lumpy skin disease is an economically important poxvirus disease of cattle. Vaccination is the main method of control but sporadic outbreaks have been reported in Turkey. This study was carried out to determine the changes in serum biochemical values of cattle naturally infected with lumpy skin disease virus (LSDV). For this study, blood samples in EDTA, serum samples, and nodular skin lesions were obtained from clinically infected animals (n = 15) whereas blood samples in EDTA and serum samples were collected from healthy animals (n = 15). A quantitative real-time PCR method was used to detect Capripoxvirus (CaPV) DNA in clinical samples. A real-time PCR high-resolution melt assay was performed to genotype CaPVs. Serum cardiac, hepatic, and renal damage markers and lipid metabolism products were measured by autoanalyzer. LSDV nucleic acid was detected in all samples which were obtained from clinically infected cattle. The results of serum biochemical analysis showed that aspartate aminotransferase, alkaline phosphatase, total protein, and creatinine concentrations were markedly increased in serum from infected animals. However, there were no significant differences in the other biochemical parameters evaluated. The results of the current study suggest that liver and kidney failures occur during LSDV infection. These findings may help in developing effective treatment strategies in LSDV infection. PMID:27294125

  1. A structural comparison of human serum transferrin and human lactoferrin.

    Science.gov (United States)

    Wally, Jeremy; Buchanan, Susan K

    2007-06-01

    The transferrins are a family of proteins that bind free iron in the blood and bodily fluids. Serum transferrins function to deliver iron to cells via a receptor-mediated endocytotic process as well as to remove toxic free iron from the blood and to provide an anti-bacterial, low-iron environment. Lactoferrins (found in bodily secretions such as milk) are only known to have an anti-bacterial function, via their ability to tightly bind free iron even at low pH, and have no known transport function. Though these proteins keep the level of free iron low, pathogenic bacteria are able to thrive by obtaining iron from their host via expression of outer membrane proteins that can bind to and remove iron from host proteins, including both serum transferrin and lactoferrin. Furthermore, even though human serum transferrin and lactoferrin are quite similar in sequence and structure, and coordinate iron in the same manner, they differ in their affinities for iron as well as their receptor binding properties: the human transferrin receptor only binds serum transferrin, and two distinct bacterial transport systems are used to capture iron from serum transferrin and lactoferrin. Comparison of the recently solved crystal structure of iron-free human serum transferrin to that of human lactoferrin provides insight into these differences. PMID:17216400

  2. Biosensor immunoassay for flumequine in broiler serum and muscle

    NARCIS (Netherlands)

    Haasnoot, W.; Gercek, H.; Cazemier, G.; Nielen, M.W.F.

    2007-01-01

    Flumequine (Flu) is one of the fluoroquinolones most frequently applied for the treatment of broilers in The Netherlands. For the detection of residues of Flu in blood serum of broilers, a biosensor immunoassay (BIA) was developed which was fast (7.5 min per sample) and specific (no cross-reactivity

  3. 9 CFR 147.2 - The rapid serum test. 2

    Science.gov (United States)

    2010-01-01

    ... 9 Animals and Animal Products 1 2010-01-01 2010-01-01 false The rapid serum test. 2 147.2 Section 147.2 Animals and Animal Products ANIMAL AND PLANT HEALTH INSPECTION SERVICE, DEPARTMENT OF AGRICULTURE LIVESTOCK IMPROVEMENT AUXILIARY PROVISIONS ON NATIONAL POULTRY IMPROVEMENT PLAN Blood...

  4. Expression and dynamic changes of Treg cells in peripheral blood and the levels of interleukin-6 in serum in HBV-related acute-on-chronic liver failure patients%乙型肝炎病毒相关慢加急性肝衰竭患者外周血调节性T细胞、血清白细胞介素-6表达及动态变化的研究

    Institute of Scientific and Technical Information of China (English)

    李晨; 王慧芬; 胡瑾华; 段学章; 万谟彬; 辛绍杰

    2012-01-01

    Objective To investigate clinical significance of Treg cells in peripheral blood and the levels of interleukin-6(IL-6) in serum in progression of HBV-related acute-on-chronic liver failure (ACLF)initial therapy patients.Methods 44 cases of HBV-related ACLF patients,46 cases of chronic hepatitis B (CHB)patients and 20 cases of healthy control (HC) people were enrolled in their respective groups.Flow cytometry was applied to detect the frequencies of Treg cells,CD39 + Treg cells,and CTLA-4 + Treg cells in peripheral blood.Enzyme linked immunosorbent assay(ELISA)was used to detect the levels of IL-6 in serum.The previous 44 cases of HBV-related ACLF patients were given a three weeks follow-up,the change trends of the frequencies of Treg cells,CD39 + Treg cells,CTLA-4 + Treg cells in peripheral blood and the levels of IL-6 in serum were observed dynamically.Results In HBV-related ACLF group,the frequencies of Treg cells,CD39 + Treg cells,CTLA-4 + Treg cells in peripheral blood and the levels of IL-6 in serum were all higher than those in CHB group and HC group(P < 0.01).In HBV-related ACLF group,the frequencies of Treg cells in peripheral blood were positively correlated with the levels of Scr(r =0.333,P < 0.05)and MELD score(r =0.388,P <0.01),the levels of IL-6 in serum were positively correlated with the levels of TBIL(r =0.470,P < 0.01),Cre (r =0.454,P < 0.01),MELD score (r =0.520,P < 0.01) and MELDNa score (r =0.506,P < 0.01),the levels of IL-6 baseline values were negatively correlatedwith Na + (r =-0.348,P < 0.05).In HBV-related ACLF group,those patients who have the higher baseline values of the frequencies of Treg cells [(6.72% ± 1.42%)] and the levels of IL-6 [(38.43 ± 31.84) pg/ml] in serum died within three weeks.This three weeks follow-up showed,in improved group,MELD score and MELD-Na showed gradually decreasing trends during improved condition with patients,the frequencies of Treg cells in peripheral blood showed a gradually rising

  5. Effect of Xiaoke Granule(消渴冲剂)on Blood Sugar and Blood Rheological Property in Experimental Diabetic Animals

    Institute of Scientific and Technical Information of China (English)

    季晓梅; 刘根尚; 齐昉; 郑虎占; 佘靖; 龚慕辛; 孙军; 章红英

    2002-01-01

    Objective: To study the pharmacological effect of Xiaoke Granule (XKG, 消渴冲剂) on blood sugar and blood rheological property in the diabetic animals.Methods: Alloxan induced diabetic mice or rats were grouped randomly. The effects of XKG on blood sugar, appetite, capacity of drinking, glucose tolerance, blood lipid and blood rheological property were observed and compared among groups.Results:XKG showed a trend in reducing the appetite and capacity of drinking, increasing the body weight, and significantly inhibiting the increase of blood sugar coused by ectogenic glucose in mice, and could improve the blood lipid and blood rheological property in rats.Conclusion:XKG is effective in reducing serum total cholesterol, lowering the blood viscosity, improving the blood rheological property of alloxan induced diabetic animals. Therefore, it might effective in treating and preventing the occurrence of diabetes mellitus and the complications of blood stasis.

  6. Drug-induced Hepatotoxicity of Anti-tuberculosis Drugs and Their Serum Levels

    OpenAIRE

    Jeong, Ina; Park, Jong-Sun; Cho, Young-Jae; Yoon, Ho Il; Song, Junghan; Lee, Choon-Taek; Lee, Jae-Ho

    2015-01-01

    The correlation between serum anti-tuberculosis (TB) drug levels and the drug-induced hepatotoxicity (DIH) remains unclear. The purpose of this study was to investigate whether anti-TB DIH is associated with basal serum drug levels. Serum peak levels of isoniazid (INH), rifampicin (RMP), pyrazinamide (PZA), and ethambutol (EMB) were analyzed in blood samples 2 hr after the administration of anti-TB medication. Anti-TB DIH and mild liver function test abnormality were diagnosed on the basis of...

  7. Lipid Profile and Serum Paraoxonase1 Activity in CRF Patients Pre and Posthemodialysis

    OpenAIRE

    Nitin S. Nagane1* and Jayshree V. Ganu2

    2011-01-01

    Chronic renal failure (CRF) is associated with an increased risk of cardiovascular disease (CVD). In the present study, 30 CRF patients undergoing hemodialysis and 50 healthy controls matching in age and sex were included. We have estimated blood urea, serum creatinine, lipid profile and serum Paraoxonase1 (PON1) activity in pre and post hemodialytic samples. HDL-Cholesterol (HDLC) and serum Paraoxonase1(PON1) activity was found to be significantly reduced (P

  8. Maternal Serum Vitamin D Levels in the Third Trimester of Pregnancy

    OpenAIRE

    PEHLİVAN, İsmail

    2002-01-01

    Aim: To measure serum vitamin D levels of pregnant women in the last trimester and to determine factors affecting serum levels. Methods: Seventy-eight pregnant women, 19-39 years old and having their third trimester between March and May 2000, were enrolled. Serum Ca, P and alkaline phosphatase and 25-hydroxyvitamin D3 levels were measured in venous blood samples during the third trimester. Maternal age, education, socioeconomic status, number of gravida, nutrition, dressing habits and daily...

  9. Second Trimester Maternal Serum Screening

    Science.gov (United States)

    ... Global Sites Search Help? Second Trimester Maternal Serum Screening Share this page: Was this page helpful? Also ... should know? How is it used? Maternal serum screening is used in the second trimester of pregnancy ...

  10. Serum Interleukin 17 Levels in Patients with Crohn's Disease: Real Life Data

    Science.gov (United States)

    Calhan, Turan; Cengiz, Mustafa; Kahraman, Resul; Aydin, Kubra; Ozdil, Kamil; Korachi, May; Sokmen, H. Mehmet

    2014-01-01

    The aim of this study was to investigate serum IL17 levels in patients with Crohn's disease (CD) and to investigate the relationship between serum IL17 levels with disease activity. Methods. Fifty patients with CD and sex- and age-matched 40 healthy controls were included in the study. The serum IL17 levels, complete blood count, blood chemistry, erythrocyte sedimentation rate (ESR), and C-reactive protein (CRP) levels were measured, and Crohn's disease activity was calculated using Crohn's disease activity index (CDAI). Results. The mean serum IL17 level of CD patients did not differ from those of healthy controls (P > 0.05). There was no difference between the mean serum IL levels of active CD patients and of quiescent CD patients (P > 0.05). However, the mean IL17 level of active patients was lower than of control subjects (P = 0.02). Serum IL17 was not correlated with inflammatory markers (ESR, CRP, white blood count, platelet count, and albumin) and CDAI. Conclusions. Peripheral blood serum IL17 levels of CD patients were not higher than of healthy controls, and also, serum IL17 level was not correlated with clinical disease activity. Peripheral IL17 measurement is not a useful tool for detecting and monitoring Crohn's disease which is understood to have complex etiopathogenesis. PMID:25140070

  11. Effect of a Marathon Run on Serum Lipoproteins, Creatine Kinase, and Lactate Dehydrogenase in Recreational Runners

    Science.gov (United States)

    Kobayashi, Yoshio; Takeuchi, Toshiko; Hosoi, Teruo; Yoshizaki, Hidekiyo; Loeppky, Jack A.

    2005-01-01

    The objective of this study was to determine the effect of a marathon run on serum lipid and lipoprotein concentrations and serum muscle enzyme activities and follow their recovery after the run. These blood concentrations were measured before, immediately after, and serially after a marathon run in 15 male recreational runners. The triglyceride…

  12. RBC aggregation dynamics in autologous plasma and serum studied with double-channel optical tweezers

    Science.gov (United States)

    Lee, Kisung; Danilina, Anna; Potkin, Anton; Kinnunen, Matti; Priezzhev, Alexander; Meglinski, Igor

    2016-04-01

    Red blood cells aggregating and disaggregating forces were measured in the autologous plasma and serum using the double-channeled optical tweezers. A significant, three-fold decrease of the both forces was observed in the serum compared to the plasma. The results of this study help to better assess the RBC aggregation mechanism.

  13. Estudio comparativo de un PCR anidado, ELISA y AGID en la detección del virus de la leucosis bovina en muestras de suero, sangre y leche Comparative study of nested PCR, ELISA and AGID tests in the detection of bovine leukaemia virus infection in serum, blood and milk samples

    Directory of Open Access Journals (Sweden)

    R Felmer

    2006-01-01

    Full Text Available Se evaluaron distintos métodos actualmente disponibles para el diagnóstico de la infección por el virus de la leucosis bovina (VLB. Los métodos empleados fueron AGID en suero, ELISA en muestras de suero y leche y PCR en linfocitos sanguíneos. De un total de 126 animales analizados, AGID identificó un menor número de animales positivos (75 comparado con las pruebas PCR y ELISA aplicadas en muestras de suero y leche (100. Tres animales positivos a AGID fueron negativos a PCR y 28 de las 51 muestras negativas a AGID fueron positivas mediante PCR. La sensibilidad diagnóstica de PCR con respecto a AGID fue de 96%, mientras que la especificidad fue de 45% (kappa 0,45. Todos los animales positivos a AGID fueron también positivos a ELISA aplicado tanto en suero como en leche, mientras que 25 animales negativos a AGID fueron consignados como positivos a ELISA, en ambas muestras biológicas. De esta forma, la sensibilidad diagnóstica de ELISA respecto a AGID fue de un 100%, mientras que la especificidad fue de 51% (kappa 0,55. La menor sensibilidad observada de AGID no es debido a reacciones falso positivas de ELISA y PCR, sino más bien a una mayor sensibilidad de estas últimas, lo que sugiere reconsiderar la utilización del método AGID en aquellos países en que aún se utiliza como método oficial en los programas de erradicación de leucosis.Different methods available for the detection of bovine leukaemia virus (BLV infection were evaluated. The methods evaluated were AGID in serum, ELISA in serum and milk, and PCR in blood lymphocytes. The AGID test identified a smaller number of positive animals (75/126 compared to PCR and ELISA tests (100/126. Three positive animals by AGID were negative by PCR and 28 of the 51 negative samples by AGID were positive by PCR. The sensitivity of PCR with respect to AGID was 96%, whereas the specificity was 45% (kappa 0.45. All positive animals by AGID were also positive by ELISA in serum and milk samples

  14. 肝肾不足型重型斑秃患者血皮质醇及糖皮质激素受体mRNA的表达状况%Serum cortisol and peripheral blood mononuclear cell glucocorticoid receptor mRNA expression in severe alopecia areata with liver-kidney deficiency syndrome

    Institute of Scientific and Technical Information of China (English)

    陈修漾; 陈达灿; 陈建宏; 危建安

    2012-01-01

    目的 探讨肝肾不足型重型斑秃患者血皮质醇、外周血单个核细胞(PBMC)中糖皮质激素受体mRNA(GRmRNA)表达状况与斑秃发病关系.方法 采用化学发光法检测23例肝肾不足型重型斑秃患者血皮质醇表达水平,并用逆转录-实时荧光定量聚合酶链反应分别检测该23例患者应用糖皮质激素(GC)治疗前后PBMC中GRmRNA表达水平,并与20例正常对照组相对比.结果 重型斑秃组与正常对照组两组血清皮质醇水平差异无统计学意义(P>0.05),重型斑秃组治疗前、后GRmRNA表达水平均低于正常对照组(P<0.05),并且治疗后GRmRNA表达水平比治疗前更低(P<0.01).结论 重型斑秃患者存在GC-GR紊乱,GRmRNA表达水平降低可能参与了重型斑秃的发病机制.肝肾不足型重型斑秃患者PBMC中GR mRNA表达水平显著低于正常对照组,这可能是重型斑秃肝肾不足证型在受体及基因水平上的病理变化,也可能是重型斑秃肝肾不足证型的微观本质之一.%Objective To investigate serum cortisol level and glucocorticoid receptors (GR) mRNA expression in peripheral blood mononuclear cells (PBMCs) in patients with severe alopecia areata and liver-kidney deficiency syndrome and their involvement in the pathogenesis of severe alopecia areata. Methods In 32 patients with severe alopecia areata, serum cortisol levels were measured by chemiluminescence assay and GR mRNA expression in the PBMCs was detected using reverse transcription real-time fluorescence quantitative PCR before and after treatment, with 20 normal subjects serving as the controls. Results Serum cortisol level showed no significant difference between the cases and the normal controls (P>0.05). The expression of GR mRNA in the PBMCs was significantly lower in the patients than in the normal controls (P<0.05). The expression of GR mRNA was even lower after treatments in patients with alopecia areata (P<0.01). Conclusion GC-GR disorder exists in

  15. Correlation of serum neutrophil gelatinase associated lipocalin with disease severity in hypertensive disorders of pregnancy

    Directory of Open Access Journals (Sweden)

    Rekha Sachan

    2014-01-01

    Full Text Available Background: Vascular endothelial dysfunction is considered central to the pathogenesis of hypertensive disorders of pregnancy (HDP. Serum level of neutrophil gelatinase-associated lipocalin (NGAL is closely related to endothelial injury. The aim of this study was to examine the correlation of serum NGAL with disease severity in HDP. Materials and Methods: This prospective case-control study was carried out for one year. After informed consent, ethical clearance, total 1,850 pregnant women were screened. Analysis was performed on 142 cases of HDP and 31 healthy controls. Quantitative measurement of serum NGAL levels was done by the enzyme linked immunosorbent assay (ELISA technique, by using sandwich ELISA kit. Results: Mean serum NGAL value in patients with oliguria was significantly higher when compared with non-oliguric patients (P < 0.001. Serum NGAL had a positive correlation with systolic blood pressure (r ~ 0.5973, diastolic blood pressure (r ~ 0.6195, blood urea (r ~ 0.4392, serum creatinine (r ~ 0.6112, serum uric acid (r ~ 0.3878. Sensitivity and specificity of serum NGAL using a cut-off value of 545 pg/ml, for the diagnosis of HDP, was 97.89% and 93.55% respectively, using 95% confidence interval. Conclusion: Between the two groups, we found that serum NGAL had a positive correlation with disease severity and better sensitivity and specificity in the evaluation of HDP.

  16. Effect of Fruit Juice on Cholesterol and Blood Pressure in Adults: A Meta-Analysis of 19 Randomized Controlled Trials

    OpenAIRE

    Liu, Kai; Xing, Anhui; Chen, Ka; Wang, Bin; Zhou, Rui; Chen, Shihui; Xu, Hongxia; Mi, Mantian

    2013-01-01

    Background The effect of fruit juice on serum cholesterol and blood pressure in humans has generated inconsistent results. We aimed to quantitatively evaluate the effect of fruit juice on serum cholesterol and blood pressure in adults. Methods We performed a strategic literature search of PubMed, EMBASE, and the Cochrane Library (updated to October, 2012) for randomized controlled trials that evaluated the effects of fruit juice on serum cholesterol and blood pressure. Study quality was asses...

  17. Perfil hematológico, bioquímico sérico, proteína C reativa e cortisol de ararajubas (Guaroba guarouba mantidas em cativeiro Blood profile, serum biochemistry, C-reactive protein and cortisol in golden conures (Guaroba guarouba in captivity

    Directory of Open Access Journals (Sweden)

    Cleyton C.D. Carvalho

    2013-03-01

    Full Text Available Objetivou-se determinar os valores hematológicos, bioquímicos séricos, proteína C reativa e cortisol de 14 ararajubas (Guaroba guarouba clinicamente saudáveis mantidas em cativeiro no zoológico do Parque Estadual de Dois Irmãos Recife/PE. Amostras de sangue foram obtidas da veia jugular com volume médio de 0,8ml, fracionando em duas porções, a primeira depositada em tubo MiniCollect®CE contendo EDTA e a outra tubo com gel separador, para a separação do soro sanguíneo. Os indicadores bioquímicos e PCR foram determinados através do analisador ARCHITECT c8000. O cortisol foi analisado no Cobas E411 da Roche. Para a determinação dos números de eritrócitos e leucócitos, foi utilizada a metodologia da contagem em câmara de Neubauer. O hematócrito foi avaliado pelo método do microhematócrito e a hemoglobina pelo método da cianometahemoglobulina. Para a contagem diferencial de leucócitos, foi utilizada a técnica de Shilling. Os índices hematimétricos (VCM, HCM E CHCM foram determinados com os valores encontrados na série eritrocítica. Os dados foram caracterizados por dispersão de freqüências, utilizando-se as seguintes medidas de tendência central: Média, desvio-padrão, mediana e percentil de 25 e percentil 75. A determinação de alguns parâmetros como ferro, triglicerídeos, PCR e cortisol foram identificados como sendo pioneiramente referenciados nesta espécie de ave. Os dados são apresentados como sendo de referência para a ararajuba (Guaroba guarouba criada em cativeiro em condições similares de manejo e higidez e ainda pode contribuir para os trabalhos de conservação ex situ desta espécie.The aim of this study was to determine the hematological, serum biochemical, C-reactive protein and cortisol 14 clinically healthy golden conures (Guaroba guarouba kept in captivity at the Zoo of Parque Estadual de Dois Irmãos Recife/PE, Brazil. Blood samples were collected from the jugular vein with an average of

  18. Effect of the change of peripheral blood serum lgG and different feeding methods on the immune function of infants%婴儿外周血血清IgG变化情况及不同喂养方式对其免疫功能的影响

    Institute of Scientific and Technical Information of China (English)

    李玉姣

    2014-01-01

    目的:探讨婴儿外周血血清免疫球蛋白G( IgG)变化情况及不同喂养方式对免疫功能的影响。方法:将180例婴儿随机分为对照组(母乳喂养无抚触)、母乳喂养抚触组、人工喂养抚触组和混合喂养抚触组各45例。分别于婴儿出生后3、20、43 d,3、6个月采外周血,进行放射免疫法测定血清中IgG含量,并进一步采用APAP单抗桥酶标法测定T细胞亚群,散射法免疫浊度测定IgM、IgA含量。结果:母乳喂养抚触组IgG含量相对于其他各组下降趋势较为缓慢( P<0.05);通过检测婴儿血清中T细胞亚群补体,发现母乳喂养抚触组中CD8+与其他各组含量比较差异有统计学意义( P<0.05);采用Spearman相关分析得出婴儿血清中免疫功能相关蛋白的高低与纯母乳喂养、抚触以及年龄具有相关性( P<0.01,P<0.05)。结论:通过母乳喂养并进行一定的抚触干预,可使婴儿体内的免疫功能得到提高,降低IgG的下降速度,有利于婴儿健康成长。%Objective:To investigate the effect of the change of peripheral blood serum IgG and different feeding methods on the immune function of infants. Methods:180 infants were randomly divided into the control group( without breast feeding),breast feeding group,bottle-feeding group and miXed feeding group(45 infants in each group). The peripheral blood of the infants were drawn respectively on the 3rd,20th and 43rd and in 3 and 6 months after the infants were born,the content of IgG,T cell subsets and the content of IgM and IgA were respectively determined. Results:The contents of IgG in serum decreased and the tending was more slowly in the breast feeding group than the other groups(P<0. 05);through the detection of T cell subsets,the difference in the comparison of the content of CD8+ was sta-tistically significant between the breast feeding group and the other groups(P<0. 05);the associated protein level with immune

  19. Serum YKL-40 in risk assessment for colorectal cancer

    DEFF Research Database (Denmark)

    Johansen, Julia Sidenius; Christensen, Ib Jarle; Jørgensen, Lars Nannestad;

    2015-01-01

    to endoscopy due to symptoms or other risk factors for colorectal cancer. Blood samples were collected just before large bowel endoscopy. Serum YKL-40 was determined by ELISA. Serum YKL-40 was higher (P < 0.0001, unadjusted for confounding covariates) in subjects diagnosed with colon cancer (median......The aim of the present study was to test the hypothesis that high serum YKL-40 associates with colorectal cancer in subjects at risk of colorectal cancer. We measured serum YKL-40 in a prospective study of 4,496 Danish subjects [2,064 men, 2,432 women, median age 61 years (range, 18-97)] referred...... useful in combination with other biomarkers in risk assessment for colorectal cancer. Cancer Epidemiol Biomarkers Prev; 24(3); 621-6. ©2015 AACR....

  20. Serum YKL-40 is increased in patients with hepatic fibrosis

    DEFF Research Database (Denmark)

    Johansen, J S; Christoffersen, P; Møller, S; Price, P A; Garbarsch, C; Henriksen, Jens Henrik Sahl; Bendtsen, F

    2000-01-01

    degree of chronic liver disease and its possible relation to liver fibrosis. METHODS: Serum YKL-40 levels were determined by radioimmunoassay in 129 patients with suspected liver disease and related to histological findings and immunohistochemical staining of YKL-40 in a liver biopsy taken simultaneously...... with the blood sample. RESULTS: The median serum YKL-40 was highest in patients with alcoholic cirrhosis (532 microg/l), in particular in patients with additional alcoholic hepatitis (740 microg/l). Patients with alcoholic cirrhosis, post-hepatitic cirrhosis (425 microg/l) and non-cirrhotic fibrosis...... (330 microg/l) had significantly higher serum YKL-40 than normal subjects (102 microg/l), patients with fatty liver (195 microg/l) or patients with viral hepatitis without fibrosis (174 microg/l). Serum YKL-40 was significantly (p<0.001) related to the degree of liver fibrosis with the highest levels...

  1. Functional assay of the alternative complement pathway of rat serum

    International Nuclear Information System (INIS)

    Two functional assays of the alternative pathway of complement activation in rat serum were developed. In the first assay, conditions were established for titration of alternative pathway activity by use of the 50% hemolytic end-point of rabbit red blood cells (RaRBC) in serum treated with ethyleneglycol-bis-(beta-aminoethyl ether)-N, N'-tetraacetic acid (EGTA). The second assay of alternative pathway activity was based on the opsonization of heat-killed radiolabeled pneumococci of serotype 25 (Pn25). Opsonization of Pn25 was shown to proceed entirely via the alternative pathway in rat serum. There was excellent correlation between the results obtained with the RaRBC lysis test and those obtained with the opsonization test. Because of its technical simplicity, the RaRBC lysis test appeared to be the single most useful test of alternative pathway activity in rat serum. (Auth.)

  2. The replacement of serum by hormones in cell culture media.

    Science.gov (United States)

    Sato, G; Hayashi, I

    1976-12-01

    The replacement of serum by hormones in cell culture media. (Reemplazo del suero por hormonas en el medio de cultivo de células). Arch. Biol. Med. Exper. 10: 120-121, 1976. The serum used in cell culture media can be replaced by a mixture of hormones and some accesory blood factors. The pituitary cell line GH3 can be grown in a medium in which serum is replaced by triiodothyronine, transferrin, parathormone, tyrotrophin releasing hormone and somatomedins. Hela and BHK cell strains can also be grown in serum free medium supplemented with hormones. Each cell type appears to have different hormonal requirements yet it may found that some hormones are required for most cell types. PMID:1026199

  3. Pharmacokinetics of nobiletin and tangeretin in rat blood serum

    Science.gov (United States)

    Citrus juice is a rich source of putatively health-beneficial compounds including flavonoids, limonoids, vitamins and others. Flavonoids are phenolic compounds, or derivatives thereof, that can act as antioxidants, and thus protect against cellular oxidative damage. The high concentrations of thes...

  4. Association of ABO and Rh Blood Groups to Blood-Borne Infections among Blood Donors in Tehran-Iran.

    Directory of Open Access Journals (Sweden)

    Fatemeh Mohammadali

    2014-07-01

    Full Text Available The aim of this study was to investigate the prevalence of hepatitis B, hepatitis C, HIV and syphilis infections in blood donors referred to Tehran Blood Transfusion Center (TBTC, and determine any association between blood groups and blood- borne infections between the years of 2005 and 2011.This was a retrospective study conducted at TBTC. All of the donor serum samples were screened for HBV, HCV, HIV and syphilis by using third generation ELISA kits and RPR test. Initial reactive samples were tested in duplicate. Confirmatory tests were performed on all repeatedly reactive donations. Blood group was determined by forward and reverse blood grouping. The results were subjected to chi square analysis for determination of statistical difference between the values among different categories according to SPSS program.Overall, 2031451 donor serum samples were collected in 2005-2011. Totally, 10451 were positive test for HBV, HCV, HIV and syphilis. The overall seroprevalence of HBV, HCV, HIV, and syphilis was 0.39%, 0.11%, 0.005%, and 0.010%, respectively. Hepatitis B and HIV infections were significantly associated with blood group of donors (P 0.05.Compared with neighboring countries and the international standards, prevalence of blood-borne infections is relatively low.

  5. Analysis on the levels of blood glucose,triglyceride and serum thyroid hormone in patients with schizo phrenia%精神分裂症患者药物治疗过程中血糖三酰甘油和甲状腺素水平分析

    Institute of Scientific and Technical Information of China (English)

    张伟英; 张毓茂; 郑华珠; 江梅英; 郭玉花; 黄兰芳

    2008-01-01

    Objective To confer the leves of blood glucose(GLU) and triglyceride(TG)and serum thyroid hormone in patients with schizophrenia in the cause of treatment.Methods To survey 88 patients with schizophrenia,and assess sera concentration of GLU and TG and thyroid hormone before treatment and during week 2,week 4,week 8,week 12.Results In the cause of treatments,the levels of GLU were up-regulated slightly(P>O.05).The levels of TG rose obviously in the clozapine group (P0.05).治疗4周后,TG水平都显著升高,氯氮平组差异有统计学意义(P<0.01),利培酮组差异也有统计学意义(P<0.05).在治疗8周后,氯氮平组TG水平进一步升高(P<0.001),利培酮组TG水平也明显升高(P<0.01),此时两组FT,水平都显著降低(P<0.05),而TSH水平却显著升高(P<0.05),且患者TG与FT3、FT4水平呈显著负相关.结论 长期服用氯氮平和利培酮治疗的精神分裂症患者存在脂代谢紊乱,而这种紊乱与甲状腺素水平有显著的相关性.

  6. Radioimmunoassay of serum ferritin

    International Nuclear Information System (INIS)

    Purified human spleen ferritin was labelled with 125I. On Sepharose 6-B gel filtration four species of labelled products were separated: a component with a higher molecular weight than ferritin; a component which is eluted in the same volume as unlabelled ferritin; and two labelled compounds with molecular weights lower than ferritin. When these labelled materials were used in a double antibody radioimmunoassay, the higher molecular weight fraction showed variable and high non-specific binding and was poorly displaced by unlabelled ferritin; the fraction behaving like true ferritin gave good standard curves and showed non-specific binding of less than 1%. The remaining two components showed poor binding to rabbit antiferritin. Using labelled material from the second fraction, a double antibody radioimmunoassay capable of measuring 2μg ferritin protein/litre of serum was developed. Inter-and intra-assay variation was between 3% and 8% over a concentration range of 0 to 250 μg ferritin protein/litre. Good agreement between serum ferritin levels assayed by the present method and by an immunoradiometric method was obtained. Labelled ferritin was stable for at least six weeks. The simplicity of the methodology makes it possible to assay serum ferritin in large batches. (author)

  7. Radioimmunoassay for serum ferritin

    International Nuclear Information System (INIS)

    We describe the development and evaluation of a serum ferritin radioimmunoassay, in which 125I-labeled ferritin and rabbit anti-ferritin antibody are used. Goat anti-rabbit gamma-globulin antibody, together with polyethylene glycol, is used as the separating reagent. The assay has a working range up to 500 μg of ferritin per litre, and a sample requirement of 75 μl of serum for assay at two dilutions. The assay requires 24 h. It has a sensitivity of 1.5 μg of ferritin per litre and a long-term precision (CV) of 13%. Reference intervals for a population of men were 18 to 330 μg/litre, with no marked age dependence, while those for a population of women older than 50 years were 18 to 200 μg/litre. Many apparently healthy women in the 20 to 50 year age group have much lower concentrations. Serum ferritin concentrations of <18 μg/litre are indicative of iron deficiency, defined as the absence of stainable iron in an aspirate of bone marrow

  8. 降糖消脂片对2型糖尿病大鼠降糖降脂作用的研究%Study of Jiangtang Xiaozhi Tablets on Decreasing Levels of Blood Glucose and Serum Lipid in Rats with Type 2 Diabetes Mellitus

    Institute of Scientific and Technical Information of China (English)

    金龙; 葛争艳; 郭宇洁; 闫爱国; 董小霞; 李宏坤; 任烨; 刘建勋

    2012-01-01

    目的:观察降糖消脂片对2型糖尿病(T2DM)模型大鼠的降糖降脂作用,为临床治疗T2DM提供实验依据.方法:采用高脂高糖饲料喂饲大鼠4周,诱发胰岛素抵抗(IR),再按低剂量链脲佐菌素(streptozotocin,STZ)25 mg·kg-1 ip,诱发高血糖症,造成T2DM大鼠模型.将实验动物分为正常对照组、模型组、降糖消脂片高、中、低(8,4,2 g·kg-1)剂量组、二甲双胍片(0.2 g·kg-1)组和金芪降糖片(2 g·kg-1)组,连续ig给药8周后杀检,测定空腹血糖(FBG)、总胆固醇(TC)、甘油三酯(TG)、胰岛素(INS)、肿瘤坏死因子(TNF-α)及进行胰腺和肝脏的病理学检查.结果:大鼠在高脂高糖饮食4周时发生高胰岛素血症伴TC增高,成功诱发出IR.STZ ip后造成T2 DM大鼠模型.口服8周后,与模型组比较,降糖消脂片高剂量组FBG明显降低(P<0.01),降糖率达到31.23%,INS水平显著增高(P<0.05),TG明显降低(P<0.01),中剂量组TG及TC明显降低(P<0.01~P<0.05),各剂量组TNF-α有降低的趋势;病理结果显示,与模型组比较,高剂量组胰岛数明显增多(P<0.05),中、低剂量组肝脂肪变性明显改善(P<0.05).结论:降糖消脂片对T2DM模型大鼠有明显的降糖、降脂的作用.%Objective: To explore the effect of Jiangtang Xiaozhi (JTXZ) tablets on decreasing levels of blood glucose and serum lipid in rats with type 2 diabetes and provide the experimental basis for the clinical therapy of type 2 diabetic mellitus (T2DM). Method: The rats were fed on a diet enriched in fat and glucose to induce insulin resistant. And a low dose streptozotocin ( STZ) was injected intrapertoneally (25 mg · kg-1 ) to induce hyperglycemia to establish T2DM. The experimental rats were divided into normal control group, model group, JTXZ tablets groups (8,4 and 2 g ·kg-1 ) , metformin group (0. 2 g·kg-1) and Jinqi Jiangtang tablets group (2 g·kg-1). After all the treatment groups were received their own medicine for 8 weeks

  9. SERUM TRACE ELEMENTS IN CHILDREN ON MAINTENANCE HEMODIALYSIS

    Directory of Open Access Journals (Sweden)

    S. T. Esfahani

    2007-08-01

    Full Text Available During dialysis some trace elements can accumulate while others may be removed from blood, leading to deficiency of some trace elements. For evaluating changes of serum trace elements in children on maintenance hemodialysis we measured copper (Cu, zinc (Zn, cobalt (Co, manganese (Mn, chromium (Cr and nickel (Ni in 3 groups of children: Group 1, children with CRF who were on regular hemodialysis; Group 2, children with CRF who were on conservative management, and Group 3, healthy children. For evaluating the impact of duration of dialysis on serum trace elements, group 1 patients were divided into two subgroups: A, patients who were on hemodialysis therapy for shorter than 18 months, and B, patients who were on hemodialysis therapy for longer than 18 months. The technique used for measurement of trace elements was PIXE (Proton Induced X-ray Emission. Mean serum levels of Zn, Mn, and Ni in group 1 were lower than group 2 and group 3. There were not significant differences in serum levels of Zn, Mn and Ni between group 2 and 3. The differences in serum levels of Cr, Co and Cu among 3 groups were not significant. The serum levels of Zn, Mn and Ni were significantly lower in subgroup B compared to subgroup A. Correlation test showed that there were an inverse linear relation between the period of hemodialysis and serum levels of Zn, Mn and Ni. Chronic hemodialysis leads to abnormalities of some trace elements in children, and these derangements increase with duration of hemodialysis.

  10. Some metabolic and anthropometric variables in obes children by measuring serum insulin, and leptin

    International Nuclear Information System (INIS)

    The present study aimed to assess serum leptin level in obese children to study its correlation with some metabolic variables as serum insulin and serum glucose. The study was conducted on 30 obese children of age from 9-14 years with body mass index (BMI) > 27.8 Kg/m2. All children were subjected to history taking, clinical examination, anthropometric measurements and laboratory investigations including fasting serum leptin, insulin and blood glucose. Serum leptin was significantly higher in obese children (102.3± 56.2 ng/ml) compared to non-obese ones (48.15±26.1 ng/ml). The relation between serum leptin and anthropometric measurements and laboratory investigations including fasting serum insulin and blood glucose. Serum leptin was significantly higher in obese children (102.3± 56.2 ng/ml)compared to non-obese ones (48.15±26.1 ng/ml). The relation between serum leptin and anthropometric variables was positively correlated with BMI rs = 0.68, (ps = 0.59.(p<0.01). It is concluded that serum leptin is increased in obesity and its concentration effects the size of the body. Moreover, the relation of leptin and insulin suggests a positive role of leptin in insulin resistance, which are common metabolic disorders associated with obesity

  11. Functional Human Blood Protein Obtained from Rice

    Institute of Scientific and Technical Information of China (English)

    2011-01-01

    Under a research project funded by NSFC,Dr.Yang He of College of Life Sciences,Wuhan University obtained functional human blood protein from rice,and published their research findings in an article "Large-scale production of functional human serum albumin from transgenic rice seeds" on PNAS in November 2011.

  12. Blood Transfusion

    Science.gov (United States)

    ... to infections including those we develop from our vaccinations (such as poliovirus antibodies, which are made by ... the Transfusion Medicine Unit, Blood Bank, and Stem Cell Storage Facility University of Rochester Medical ... and health educators who are available by phone Monday through Friday, 9 am to 9 pm ( ...

  13. Influence of common preanalytical variations on the metabolic profile of serum samples in biobanks

    Energy Technology Data Exchange (ETDEWEB)

    Fliniaux, Ophelie [University of Picardie Jules Verne, Laboratoire de Phytotechnologie EA 3900-BioPI (France); Gaillard, Gwenaelle [Biobanque de Picardie (France); Lion, Antoine [University of Picardie Jules Verne, Laboratoire de Phytotechnologie EA 3900-BioPI (France); Cailleu, Dominique [Batiment Serres-Transfert, rue de Mai/rue Dallery, Plateforme Analytique (France); Mesnard, Francois, E-mail: francois.mesnard@u-picardie.fr [University of Picardie Jules Verne, Laboratoire de Phytotechnologie EA 3900-BioPI (France); Betsou, Fotini [Integrated Biobank of Luxembourg (Luxembourg)

    2011-12-15

    A blood pre-centrifugation delay of 24 h at room temperature influenced the proton NMR spectroscopic profiles of human serum. A blood pre-centrifugation delay of 24 h at 4 Degree-Sign C did not influence the spectroscopic profile as compared with 4 h delays at either room temperature or 4 Degree-Sign C. Five or ten serum freeze-thaw cycles also influenced the proton NMR spectroscopic profiles. Certain common in vitro preanalytical variations occurring in biobanks may impact the metabolic profile of human serum.

  14. Kadar Asam Urat Serum sebagai Biomarker Preeklamsi

    Directory of Open Access Journals (Sweden)

    Neli Sumanti

    2013-06-01

    Full Text Available Preeclampsia remains a health problem that becomes one of the causes of maternal deaths besides bleeding and infection. The etiology and pathogenesis of preeclampsia are unclear. Increased serum uric acid levels is seen simultaneously with the increase of blood pressure and occurred before the onset of proteinuria. Therefore, the uric acid can be used as a biomarker. The aim of this study was to analyze the serum uric acid levels between normal and preeclampsia pregnancies. The study was conducted in Dr.Hasan Sadikin Hospital Bandung between March and May 2011, using cross sectional study design. Subjects were 45 inpartu normal pregnant women as control and 44 in partu pregnant women with preeclampsia accordance with inclusion and exclusion criteria. Levels of uric acid in normal pregnancy are 3,43 ±0.14 mg/dL. In this study uric acid levels resulting in cut-off levels of 4,8 mg/dL with a sensitivity value of 93%, and specificity 80%. Conclusions: uric acid levels in at term preeclampsia are higher compared with normal pregnancies. Increased levels of uric acid can be considered as one of biomarkers of preeclampsia, hence the serum uric acid levels used as serial examinations in pregnant women during antenatal care.

  15. Measurement of proinsulin in human serum

    Energy Technology Data Exchange (ETDEWEB)

    Sato, Keizo; Takanohashi, Kenji; Murozono, Soichi; Ishii, Hikaru.

    1987-08-01

    In this study, we developed a measuring system for proinsulin in human serum. Insulin-like substances (ILS) were isolated from serum by an affinity chromatography, and proinsulin in ILS was separated from insulin by high-performance liquid chromatography (HPLC). For the radioimmunoassay (RIA), porcine proinsulin was used as the standard and as /sup 125/I-labelled antigen, and porcine insulin anti-serum was as the antibody. Proinsulin-like immunoreactivity (PLI) can be estimated directly from a standard curve, and the detection limit of PLI is 0.0044 pmol/ml. By this method, the values of PLI and insulin-like immunoreactivity (IRI) in human blood were able to estimate precisely. PLI values in the fasting state and in 30, 60, 90, and 120 min after oral glucose stimulation from nine healthy subjects were 0.011 +- 0.002, 0.045 +- 0.014, 0.078 +- 0.019, 0.064 +- 0.016, and 0.027 +- 0.006 pmol/ml, respectively. The increase in PLI response was observed later than that in IRI response. PLI/total IRI ratio in the fasting state and 60 min after oral glucose were 24 and 36 % respectively.

  16. Measurement of proinsulin in human serum

    International Nuclear Information System (INIS)

    In this study, we developed a measuring system for proinsulin in human serum. Insulin-like substances (ILS) were isolated from serum by an affinity chromatography, and proinsulin in ILS was separated from insulin by high-performance liquid chromatography (HPLC). For the radioimmunoassay (RIA), porcine proinsulin was used as the standard and as 125I-labelled antigen, and porcine insulin anti-serum was as the antibody. Proinsulin-like immunoreactivity (PLI) can be estimated directly from a standard curve, and the detection limit of PLI is 0.0044 pmol/ml. By this method, the values of PLI and insulin-like immunoreactivity (IRI) in human blood were able to estimate precisely. PLI values in the fasting state and in 30, 60, 90, and 120 min after oral glucose stimulation from nine healthy subjects were 0.011 ± 0.002, 0.045 ± 0.014, 0.078 ± 0.019, 0.064 ± 0.016, and 0.027 ± 0.006 pmol/ml, respectively. The increase in PLI response was observed later than that in IRI response. PLI/total IRI ratio in the fasting state and 60 min after oral glucose were 24 and 36 % respectively. (author)

  17. The radioimmunoassay of human serum inhibin

    International Nuclear Information System (INIS)

    The measurement of circulating inhibin is essential to the advancement of our understanding of its role in the control of FSH secretion. Recently, sensitive in vitro bioassay systems have been described which have allowed the measurement of inhibin bioactivity in the peripheral serum of immature female rats, sheep and women (C. Tsonis, personal communication) following ovarian hyperstimulation with exogenous gonadotrophins. Inhibin bioactivity has also been shown to rise in ovarian venous blood in the late follicular phase of women and during FSHG administration to primates. Such studies have some important limitations, in particular, the bioassay systems employed lack the sensitivity required for the study of normal physiology, and questions regarding the specificity of in vitro bioassay in application to serum samples have arisen following the reports of FSH releasing factors (transforming growth factor-beta inhibin B subunit dimers) that may confound the accurate assessment of inhibin bioactivity. This paper reports on the development of sensitive and specific radioimmunoassay (RIA) systems applicable to human serum utilizing highly purified preparations of 58kDa and 31kDa bovine follicular fluid (bFF) inhibin. The development of two RIA systems utilizing antisera raised to either or 31kDa bFF inhibin is detailed and their application to the study of inhibin physiology in the human female during ovulation inducting, the normal menstrual cycle and gestation described

  18. Blood Culture (For Parents)

    Science.gov (United States)

    ... KidsHealth in the Classroom What Other Parents Are Reading Upsetting News Reports? What to Say Vaccines: Which ... BMP) Blood Test: Complete Blood Count Basic Blood Chemistry Tests Getting a Blood Test (Video) Blood Test: ...

  19. High Blood Pressure (Hypertension)

    Science.gov (United States)

    ... your doctor prescribes it, medicine. What Is Blood Pressure? Blood pressure is the force of blood flow inside ... Will I Know if I Have High Blood Pressure? High blood pressure is a silent problem — you won't ...

  20. Blood Pressure Quiz

    Science.gov (United States)

    ... page please turn Javascript on. Feature: High Blood Pressure Blood Pressure Quiz Past Issues / Fall 2011 Table of Contents ... About High Blood Pressure / Treatment: Types of Blood Pressure Medications / Blood Pressure Quiz Fall 2011 Issue: Volume 6 Number ...

  1. Hypertension (High Blood Pressure)

    Science.gov (United States)

    ... right away. continue How Do Doctors Measure Blood Pressure? Blood pressure readings are fast and painless. Blood pressure ... same age, height, and gender have lower blood pressure. Blood pressure between 90% and 95% of the normal ...

  2. Blood pressure measurement

    Science.gov (United States)

    Diastolic blood pressure; Systolic blood pressure; Blood pressure reading; Measuring blood pressure ... or your health care provider will wrap the blood pressure cuff snugly around your upper arm. The ...

  3. Blood Transfusions (For Teens)

    Science.gov (United States)

    ... How Can I Help a Friend Who Cuts? Blood Transfusions KidsHealth > For Teens > Blood Transfusions Print A ... United States get blood transfusions. A Bit About Blood As blood moves throughout the body, it carries ...

  4. Blood Count Tests

    Science.gov (United States)

    Your blood contains red blood cells (RBC), white blood cells (WBC), and platelets. Blood count tests measure the number and types of cells in your blood. This helps doctors check on your overall health. ...

  5. Blood Clotting and Pregnancy

    Medline Plus

    Full Text Available ... About Awards Membership ASH Foundation Global Programs Newsroom facebook twitter youtube linkedin Research In This Section Agenda ... View all publications For Patients Blood Basics Blood Disorders Anemia Bleeding Disorders Blood Cancers Blood Clots Blood ...

  6. Blood Clotting and Pregnancy

    Medline Plus

    Full Text Available ... For Patients Blood Disorders Blood Clots Blood Clotting & Pregnancy If you are pregnant, or you have just ... The risk of developing a blood clot during pregnancy is increased by the following: Previous blood clots ...

  7. 西洋参对低舒张压的老年高血压血清相关炎性反应因子的影响%Effect of American Ginseng on the Serum E-selectin and Soluble ICAM-1 Levels in Elderly Hypertensive Patients with Low Diastolic Blood Pressure

    Institute of Scientific and Technical Information of China (English)

    阳永扬

    2015-01-01

    Objective:To observe the effect of American ginseng on the serum E-selectin and soluble ICAM-1 levels in elderly hy-pertensive patients with low diastolic blood pressure.Methods:In the study,100 elderly hypertensive patients after regular anti-hypertensive therapy with low diastolic blood pressure,who were older than 65 years old,were randomized into two groups.The 50 patients in American ginseng group received combination therapy of American ginseng and anti-hypertensive drugs;the 50 patients in single drug group received only anti-hypertensive drugs.The sICAM-1 and sE-selectin levels in the patients’blood were meas-ured before and after the treatment.Results:The systolic blood pressures in the two groups were reduced after treatment.The A-merican ginseng group showed a greater reduction than the single drug group (P <0.05).The diastolic blood pressures were sig-nificantly increased,and pulse pressures were significantly reduced after the treatment in the American ginseng group,which was superior to the single drug group (P <0.01).There was no significant difference in the sICAM-1 and sE-selectin levels between the American ginseng group and the single drug group before the treatment.The sICAM-1 and sE-selectin levels were effectively reduced in the two groups after the treatment,but the American ginseng group showed a greater reduction in the sICAM-1 and sE-selectin levels than the single drug group (P <0.01).Conclusion:The combination therapy of American ginseng and anti-hyper-tensive drug can effectively treat elderly hypertensive patients with low diastolic blood pressure.It can protect and repair vascular endothelial cells through regulation of adhesion molecules expression and inhibition of inflammatory reaction.%目的:观察西洋参联合降压药物治疗对低舒张压的老年高血压患者血清 E-选择素及可溶性细胞间黏附分子水平的影响,并探讨西洋参治疗该疾病的机制。方法:选取100名65岁以上的经正规

  8. The Human Serum Metabolome

    OpenAIRE

    Psychogios, Nikolaos; Hau, David. D.; Peng, Jun; Guo, An Chi; Mandal, Rupasri; Bouatra, Souhaila; Sinelnikov, Igor; Krishnamurthy, Ramanarayan; Eisner, Roman; Gautam, Bijaya; Young, Nelson; Xia, Jianguo; Knox, Craig; Dong, Edison; Huang, Paul

    2011-01-01

    Continuing improvements in analytical technology along with an increased interest in performing comprehensive, quantitative metabolic profiling, is leading to increased interest pressures within the metabolomics community to develop centralized metabolite reference resources for certain clinically important biofluids, such as cerebrospinal fluid, urine and blood. As part of an ongoing effort to systematically characterize the human metabolome through the Human Metabolome Project, we have unde...

  9. Calorimetric monitoring of the serum proteome in schizophrenia patients

    International Nuclear Information System (INIS)

    Highlights: • DSC reveals modified thermal behavior of blood serum from schizophrenic patients. • The high-abundance portion of the serum proteome is thermally stabilized in Sz. • The Sz plasma thermograms are classified in four distinct calorimetric groups. • The effectiveness of drug treatment correlates with the plasma thermodynamic behavior. - Abstract: Schizophrenia (Sz) is a multifactorial mental disorder with high frequency. Due to its chronic and relapsing nature there is a strong need for biomarkers for early psychosis detection and objective evaluation of drug (usually antipsychotics) treatment effect. Here differential scanning calorimetry (DSC) is applied to thermodynamically characterize the blood serum proteome of paranoid schizophrenia patients on routine antipsychotic treatment in comparison to healthy controls. DSC revealed significant modifications in the thermodynamic behavior of blood sera from Sz patients, the overall thermal profile being changed in all Sz cases under study. The calorimetric profiles were classified in four distinct groups, reflecting different thermal stabilization of the high-abundance portion of the serum proteome. The observed positive (thermograms becoming closer to the healthy profile) or negative (thermograms deviating stronger from the healthy profile) proteome thermal stability switches and the Sz thermograms persistence in patients’ follow-up corresponded well with the effect of drug treatment

  10. Calorimetric monitoring of the serum proteome in schizophrenia patients

    Energy Technology Data Exchange (ETDEWEB)

    Krumova, Sashka [Institute of Biophysics and Biomedical Engineering, Bulgarian Academy of Sciences, Acad. G. Bonchev Str., Bl. 21, Sofia 1113 (Bulgaria); Rukova, Blaga [Department of Medical Genetics, Medical University of Sofia, 2 Zdrave Str., Sofia 1431 (Bulgaria); Todinova, Svetla [Institute of Biophysics and Biomedical Engineering, Bulgarian Academy of Sciences, Acad. G. Bonchev Str., Bl. 21, Sofia 1113 (Bulgaria); Gartcheva, Lidia [National Specialized Hospital for Active Treating of Haematological Diseases, 6 Plovdivsko pole Str., Sofia 1756 (Bulgaria); Milanova, Vihra [Department of Psychiatry, Medical University of Sofia, 1 Sv. Georgi Sofiiski Str., Sofia 1431 (Bulgaria); Toncheva, Draga [Department of Medical Genetics, Medical University of Sofia, 2 Zdrave Str., Sofia 1431 (Bulgaria); Taneva, Stefka G., E-mail: stefka.germanova@ehu.es [Institute of Biophysics and Biomedical Engineering, Bulgarian Academy of Sciences, Acad. G. Bonchev Str., Bl. 21, Sofia 1113 (Bulgaria)

    2013-11-20

    Highlights: • DSC reveals modified thermal behavior of blood serum from schizophrenic patients. • The high-abundance portion of the serum proteome is thermally stabilized in Sz. • The Sz plasma thermograms are classified in four distinct calorimetric groups. • The effectiveness of drug treatment correlates with the plasma thermodynamic behavior. - Abstract: Schizophrenia (Sz) is a multifactorial mental disorder with high frequency. Due to its chronic and relapsing nature there is a strong need for biomarkers for early psychosis detection and objective evaluation of drug (usually antipsychotics) treatment effect. Here differential scanning calorimetry (DSC) is applied to thermodynamically characterize the blood serum proteome of paranoid schizophrenia patients on routine antipsychotic treatment in comparison to healthy controls. DSC revealed significant modifications in the thermodynamic behavior of blood sera from Sz patients, the overall thermal profile being changed in all Sz cases under study. The calorimetric profiles were classified in four distinct groups, reflecting different thermal stabilization of the high-abundance portion of the serum proteome. The observed positive (thermograms becoming closer to the healthy profile) or negative (thermograms deviating stronger from the healthy profile) proteome thermal stability switches and the Sz thermograms persistence in patients’ follow-up corresponded well with the effect of drug treatment.

  11. Blood-derived topical therapy for ocular surface diseases.

    Science.gov (United States)

    Soni, Nishant G; Jeng, Bennie H

    2016-01-01

    Human serum-derived and plasma-derived therapies have become increasingly popular in the treatment of ocular surface disorders, with mounting clinical and scientific evidence suggesting good safety and efficacy profiles. These therapies may be considered for various ocular surface conditions, such as dry eye syndrome and persistent epithelial defect, when conservative management does not suffice. The costly and inconvenient process of obtaining the blood-derived products is the barrier to their more widespread use. Some blood-derived therapies, such as umbilical cord serum-derived and platelet-derived plasma preparations, may be more viable options since these therapies can be made readily available to patients. In this review, the existing literature on the safety and efficacy of blood-derived products, such as autologous serum tears, in the treatment of ocular surface diseases is discussed. Issues relevant to the production of autologous serum tears are also described. PMID:26178904

  12. Changes in serum leptin level in patients with diabetic retinopathy

    International Nuclear Information System (INIS)

    Objective: To explore the regulation of changes in serum leptin level in patients with diabetic retinopathy. Methods: The 120 participating subjects were of four groups: healthy controls, diabetic patients without retinopathy, patients with NPDR and patients with PDR, each group consisted of 18 males and 12 females with comparable BMI. The levels of serum leptin, IVC, insulin and blood glucose of these patients were measured and the correlation between serum leptin level and other parameters was analysed. Results: The level of serum leptin in controls, diabetic patients without retinopathy, patients with NPDR and patients with PDR were 6.91 ± 1.87 μg/L, 7,83 ±2.11 μg/L, 9.56 ± 2.43 μg/L and 11.69 ± 2.57 μg/L respectively. The patients with PDR had higher serum leptin levels than patients with NPDR (t=2.15, p < 0.05), diabetic patients without retinopathy (t = 2.71, p < 0.01), and controls (t = 3.50, p < 0.001), the patients with NPDR had higher serum leptin levels than diabetic patients without retinopathy (t = 2.23, p < 0.05) and controls (t = 2.75, p < 0.01), while the difference in serum leptin was not significant between diabetic patients without retinopathy and controls. The serum level was positively correlated to BMI (r = 0.22, p < 0.05) and FINS (r = 0.28, p < 0.01). Conclusion: Serum leptin level is elevated in patients with diabetic retinopathy and is positively correlated to the severity of the disease

  13. Free serum thyroxine

    International Nuclear Information System (INIS)

    The use of radiommunoassay (RIA) tehcniques has increased the diagnosis of thyroid functional alterations. A solid phase RIA method for free thyroxine (FT4) measurement was tested. Serum FT4, Total T4, T3 and TSH were determined by radioimmunoassay in 179 subjects. One hundred twenty two patients were normal (8 to 75 years old); FT4 was 1.42 +- 0.03 ng/100 ml (avg. value and std. error). In 27 cases of thyrotoxicosis the values were 4.66 +- 0.48 ng/100 ml and in 15 cases of hypothyroidism 0.50 +- 0.06 ng/100 ml (statistics probability <0,01). Four euthyroid patients under estrogen treatment, 9 hypothyroid patients under l-thyroxine treatment, one hyperthyroid patient under antithyroid drugs and one hyperthyroid patient under estrogen treatment, were studied. Total T4 and T3 values were in agreement with the variations of the serum protein transport capacity, and for that reason did not show the real thyroid function state. Clinical decision making analysis (with Receiver Operating Characteristic (ROC) curves) indicate that FT4 is the best choice for hyperthyroidism diagnosis and T4 for hypothyroidism. Cost/benefit analysis indicates also that FT4 has the best ratio. (author)

  14. Effects of coagulation temperature on measurements of complement function in serum samples from patients with systemic lupus erythematosus

    DEFF Research Database (Denmark)

    Baatrup, G; Sturfelt, G; Junker, A; Svehag, S E

    1992-01-01

    Blood samples from 15 patients with systemic lupus erythematosus (SLE) and 15 healthy blood donors were allowed to coagulate for one hour at room temperature, followed by one hour at 4 or 37 degrees C. The complement activity of the serum samples was assessed by three different functional assays......, determined by high performance gel permeation chromatography, was also similar. This study shows that the results of functional complement assays, applied to serum samples from patients with SLE cannot be compared unless the conditions for blood coagulation and serum handling are defined and are the same...

  15. Relationship of endothelial nitric oxide synthase gene polymorphism with blood pressure,lipid profile and blood glucose level

    Institute of Scientific and Technical Information of China (English)

    2008-01-01

    To study the relationship of the polymorphism of endothelial nitric oxide synthase(eNOS)gene and blood pressure,lipid profiles and blood glucose level.By using PCR-RFLP,the eNOS Glu298Asp gene polymorphism was detected in 184 patients with essential hypertension and 196 matched healthy individuals with normal blood pressure.Taking into account eNOS Glu298Asp polymorphisms,the relationship of blood pressure with triglycerides(TG),total cholesterol(TC),high density lipoprotein(HDL),low density lipoprotein(LDL)and blood glucose level was analyzed.The distribution of eNOS Glu298Asp polymorphism had no significant difference between different blood pressure groups and gender groups,but there was a significant difference between different age groups,diastolic blood pressure groups or BMI groups(P<0.05).Asp/Asp genotype significantly increased the risk of hypertension in individuals with serum TC above 5.4 mmol/L(P=0.03,OR=2.65).eNOSGlu298Asp polymorphism and serum lipid could synergistically modulate the blood pressure,eNOS Asp/Asp genotype could significantly increase the risk of hypertension in individuals with serum TC over 5.4 mmol/L,eNOS Glu298Asp in combination with serum TC could be used to predict the risk of hypertension.

  16. Serum adiponectin concentrations in patients with essential hypertension

    Institute of Scientific and Technical Information of China (English)

    LI Xiao-ping; YANG Cheng-ti; GAO Xing-yu; ZHANG Ling; HU Hou-xiang; CHEN Li

    2004-01-01

    To explore the serum levels of adiponectin in patients with essential hypertension and the relationbetween adiponectin and blood pressure. Methods: Forty-five cases with essential hypertension and 43 healthy control sub-jects have been taken fasting blood samples for measurements of plasma glucose, plasma lipids, insulin, C-peptide, thyroidhormones, TNF-α, leptin and adiponectin. Glucose tolerance was assessed by 75-g oral glucose tolerance est. Results: Theconcentrations of adiponectin in cases with essential hypertension were significantly lower than those in the control group(4.15 ± 1.99 vs 7.04 ± 3.13 mg/ml, P = 0.000). Pearson relation analysis showed that serum adiponectin concentrationswere negatively and significantly correlated with body-mass index ( r = - 0. 274, P = 0. 038 ), total cholesterol(r = -0.257, P = 0.048)in control groups, while adiponectin concentrations were negatively and significantly correlatedwith systolic blood pressure ( r = - 0.356, P = 0.016), triglyceride ( r = - 0.367, P = 0.013), tumor necrosis factor-al-pha ( r = - 0. 298, P = 0.047) and triiodothyronine ( r = - 0.317, P = 0. 034) in essential hypertension group. Multipleregression analysis showed that body-mass index was the independent factor to adiponectin levels, and SBP and TNF-α wereadiponectin independent factors in the essential hypertension group. Conclusion: The serum adiponectin concentrations aresignificant lower in patients with essential hypertension, and there is negative and significantly correlation between adiponec-tin and blood pressure.

  17. Filariasis and serum specific gravity

    OpenAIRE

    Viroj Wiwanitkit

    2009-01-01

    "nFilariasis is a problematic tropical vector borne infection. Here, the author proposes an idea on a physical change, serum specific gravity, in serum of filariasis cases and further extrapolates for its clinical usefulness.  According to this study, the finalized estimated serum specificity in filariasis is more than that of normal condition. The change of the specific gravity due to additional content or mass can be demonstrated and might be useful for diagnosis and following...

  18. Serum stimulation of plasma protein synthesis in culture is selective and rapidly reversible.

    Science.gov (United States)

    Plant, P W; Liang, T J; Pindyck, J; Grieninger, G

    1981-10-27

    Primary hepatocyte monolayers, derived from chick embryos, can be cultured from the onset in a completely chemically defined medium, free of added hormones. The liver cells synthesize and secrete a wide spectrum of plasma proteins for several days in this serum-free environment. Addition of fetal bovine serum elicits a 3-5-fold increase in the production of certain plasma proteins: fibrinogen, albumin, and the alpha1-globulin M. This effect of serum is selective; transferrin and plasminogen syntheses are enhanced less than 1.5-fold. Significant stimulation is observed with 0.1% fetal bovine serum, and half-maximal values for individual plasma proteins are obtained with concentrations ranging between 0.4 and 1%. The stimulatory activity of serum shows no developmental or species specificity. Plasma is active as serum derived from the same blood sample. The hepatocytes respond rapidly to serum, significant changes in albumin synthesis occurring less than 1 h after serum addition or removal. The effect of short exposure is fully reversible. These results establish the capacity of low concentrations of serum to stimulate plasma protein synthesis and underscore the importance of studying the effects of hormones and other factors under serum-free conditions. The findings suggest that, in addition to the classical hormones, ubiquitous but as yet uncharacterized serum components play a role in controlling this major hepatic function. PMID:7284395

  19. Separation of blood in microchannel bends

    Science.gov (United States)

    Blattert, Christoph; Jurischka, Reinhold; Schoth, Andreas; Kerth, Paul; Menz, Wolfgang

    2004-01-01

    Biological applications of micro assay devices require integrated on-chip microfluidics for separation of plasma or serum from blood. This is achieved by a new blood separation technique based on a microchannel bend structure developed within the collaborative Micro-Tele-BioChip (μTBC) project co-funded by the German Ministry For Education and Research (BMBF). Different prototype polymer chips have been manufactured with an UV-LIGA process and hot embossing technology. The separation efficiency of these chips has been determined by experimental measurements using human whole blood. Results show different separation efficiencies for cells and plasma depending on microchannel geometry and blood sample characteristics and suggest an alternative blood separation method as compared to existing micro separation technologies.

  20. Detection of cryoglobulins in serum of Caspian miniature horse

    Directory of Open Access Journals (Sweden)

    Atyabi, N,

    2012-06-01

    Full Text Available Blood samples were collected from 20 healthy miniature Caspian horses at 37 °C. Isolation of cryoglobulin was performed based on a standard method in present study. Harvested sera were kept at 4 °C for two hours and then examined for cryoglubolin. Four serum samples containing precipitate Suspicious of containing cryoglobulin were selected. Subsequently serum protein electrophoresis was performed on normal serum samples and also on four serum samples containing precipitates using an automated electrophoresis system on cellulose acetate strips. In addition Ig isotypes detection (IgG, IgM and IgA was performed on both precipitates and serum containing precipitates using single radio immunediffusion method (SRID. A narrow-based peak on gamma region of precipitate acetate cellulose strips was observed. Precipitate concentrations were strikingly higher than normal concentration of serum immuneglobulins. It can be suggested that cryoglobulin concentration in a proportion of Caspian miniature horse is higher than other equides may be in relation with animal susceptibility to neoplasias such as lymphoma and leukemia.