WorldWideScience

Sample records for blood related products

  1. Impact of bleeding-related complications and/or blood product transfusions on hospital costs in inpatient surgical patients

    Directory of Open Access Journals (Sweden)

    Reynolds Matthew W

    2011-05-01

    Full Text Available Abstract Background Inadequate surgical hemostasis may lead to transfusion and/or other bleeding-related complications. This study examines the incidence and costs of bleeding-related complications and/or blood product transfusions occurring as a consequence of surgery in various inpatient surgical cohorts. Methods A retrospective analysis was conducted using Premier's Perspective™ hospital database. Patients who had an inpatient procedure within a specialty of interest (cardiac, vascular, non-cardiac thoracic, solid organ, general, reproductive organ, knee/hip replacement, or spinal surgery during 2006-2007 were identified. For each specialty, the rate of bleeding-related complications (including bleeding event, intervention to control for bleeding, and blood product transfusions was examined, and hospital costs and length of stay (LOS were compared between surgeries with and without bleeding-related complications. Incremental costs and ratios of average total hospital costs for patients with bleeding-related complications vs. those without complications were estimated using ordinary least squares (OLS regression, adjusting for demographics, hospital characteristics, and other baseline characteristics. Models using generalized estimating equations (GEE were also used to measure the impact of bleeding-related complications on costs while accounting for the effects related to the clustering of patients receiving care from the same hospitals. Results A total of 103,829 cardiac, 216,199 vascular, 142,562 non-cardiac thoracic, 45,687 solid organ, 362,512 general, 384,132 reproductive organ, 246,815 knee/hip replacement, and 107,187 spinal surgeries were identified. Overall, the rate of bleeding-related complications was 29.9% and ranged from 7.5% to 47.4% for reproductive organ and cardiac, respectively. Overall, incremental LOS associated with bleeding-related complications or transfusions (unadjusted for covariates was 6.0 days and ranged from 1

  2. Central and peripheral blood pressures in relation to plasma advanced glycation end products in a Chinese population.

    Science.gov (United States)

    Huang, Q-F; Sheng, C-S; Kang, Y-Y; Zhang, L; Wang, S; Li, F-K; Cheng, Y-B; Guo, Q-H; Li, Y; Wang, J-G

    2016-07-01

    We investigated the association of plasma AGE (advanced glycation end product) concentration with central and peripheral blood pressures and central-to-brachial blood pressure amplification in a Chinese population. The study subjects were from a newly established residential area in the suburb of Shanghai. Using the SphygmoCor system, we recorded radial arterial waveforms and derived aortic waveforms by a generalized transfer function and central systolic and pulse pressure by calibration for brachial blood pressure measured with an oscillometric device. The central-to-brachial pressure amplification was expressed as the central-to-brachial systolic blood pressure difference and pulse pressure difference and ratio. Plasma AGE concentration was measured by the enzyme-linked immunosorbent assay method and logarithmically transformed for statistical analysis. The 1051 participants (age, 55.1±13.1 years) included 663 women. After adjustment for sex, age and other confounding factors, plasma AGE concentration was associated with central but not peripheral blood pressures and with some of the pressure amplification indexes. Indeed, each 10-fold increase in plasma AGE concentration was associated with 2.94 mm Hg (P=0.04) higher central systolic blood pressure and 2.39% lower central-to-brachial pulse pressure ratio (P=0.03). In further subgroup analyses, the association was more prominent in the presence of hypercholesterolemia (+8.11 mm Hg, P=0.008) for central systolic blood pressure and in the presence of overweight and obesity (-4.89%, P=0.009), diabetes and prediabetes (-6.26%, P=0.10) or current smoking (-6.68%, P=0.045) for central-to-brachial pulse pressure ratio. In conclusion, plasma AGE concentration is independently associated with central systolic blood pressure and pulse pressure amplification, especially in the presence of several modifiable cardiovascular risk factors. PMID:26084655

  3. EFSA Panel on Dietetic Products, Nutrition and Allerg ies (NDA) ; Guidance on the scientific requirements for health claims related to appetite ratings, weight management, and blood glucose concentrations

    DEFF Research Database (Denmark)

    Tetens, Inge

    The European Food Safety Authority (EFSA) asked the Panel on Dietetic Products, Nutrition and Allergies (NDA) to draft guidance on scientific requirements for health claims related to appetite ratings, weight management, and blood glucose concentrations. This guidance has been drawn from scientific...

  4. Infections Transmitted By the Transfusion of Blood and Blood Products

    OpenAIRE

    Tekin A.

    2011-01-01

    Especially viral hepatitis viruses and human immunodeficiency virus(HIV) which were transmitted by the transfusion of blood and blood products have been an important public health problem for a long time on the world. Transfusion of blood and blood products is an ideal and an easiest and a simplest route for transmission of infectious diseases. It is known that many infectious agents, either bacterial, viral, parasitic and fungal agents may be transmitted by the transfusion of blood and blood...

  5. Infections Transmitted By the Transfusion of Blood and Blood Products

    Directory of Open Access Journals (Sweden)

    Tekin A.

    2011-05-01

    Full Text Available Especially viral hepatitis viruses and human immunodeficiency virus(HIV which were transmitted by the transfusion of blood and blood products have been an important public health problem for a long time on the world. Transfusion of blood and blood products is an ideal and an easiest and a simplest route for transmission of infectious diseases. It is known that many infectious agents, either bacterial, viral, parasitic and fungal agents may be transmitted by the transfusion of blood and blood products. In present study, we reviewed infection diseases that transmitted by the transfusion of blood and blood products.Additionally, we were aimed to emphasize a rare but a very important complication of transfusion of blood and blood products.

  6. Use of blood and blood products.

    Science.gov (United States)

    Hunt, E; Wood, B

    1999-11-01

    It is sometimes necessary for the practitioner to transfuse the ruminant with whole blood or plasma. These techniques are often difficult to perform in practice, are time-consuming, expensive, and stressful to the animal. Acute loss of 20% to 25% of the blood volume will result in marked clinical signs of anemia, including tachycardia and maniacal behavior. The PCV is only a useful tool with which to monitor acute blood loss after intravascular equilibration with other fluid compartments has occurred. An acutely developing PCV of 15% or less may require transfusion. Chronic anemia with PCV of 7% to 12% can be tolerated without transfusion if the animal is not stressed and no further decline in erythrocyte mass occurs. Seventy-five percent of transfused bovine erythrocytes are destroyed within 48 hours of transfusion. A transfusion rate of 10 to 20 mL/kg recipient weight is necessary to result in any appreciable increase in PCV. A nonpregnant donor can contribute 10 to 15 mL of blood/kg body weight at 2- to 4-week intervals. Sodium citrate is an effective anticoagulant, but acid citrate dextrose should be used if blood is to be stored for more than a few hours. Blood should not be stored more than 2 weeks prior to administration. Heparin is an unsuitable anticoagulant because the quantity of heparin required for clot-free blood collection will lead to coagulation defects in the recipient. Blood cross-matching is only rarely performed in the ruminant. In field situations, it is advisable to inject 200 mL of donor blood into the adult recipient and wait 10 minutes. If no reaction occurs, the rest of the blood can probably be safely administered as long as volume overload problems do not develop. Adverse reactions are most commonly seen in very young animals or pregnant cattle. Signs of blood or plasma transfusion reaction include hiccoughing, tachycardia, tachypnea, sweating, muscle tremors, pruritus, salivation, cough, dyspnea, fever, lacrimation, hematuria

  7. Transfusions of blood and blood products and viral infections

    Directory of Open Access Journals (Sweden)

    Marta Wróblewska

    2002-06-01

    Full Text Available Transfusions of blood and blood products are commonly used in medicine, but being biological materials they carry a risk of transmitting infections--viral, bacterial, parasitic, as well as prions. Laboratory tests used for screening of donated blood for viral infections at present cannot detect all infectious units. Criteria for selection of blood donors therefore must be very strict, while methods of inactivation of viruses and laboratory assays for detection of their presence must be improved. Indications for blood transfusion should be restricted.

  8. Determination of Rate and Causes of Wastage of Blood and Blood Products in Iranian Hospitals

    Directory of Open Access Journals (Sweden)

    Rafat Mohebbi Far

    2014-06-01

    Full Text Available OBJECTIVE: The purpose of this study was to determine the rate and causes of wastage of blood and blood products (packed red cells, plasma, platelets, and cryoprecipitate in Qazvin hospitals. METHODS: The study was conducted in all hospitals in Qazvin, including 5 teaching hospitals, 2 social welfare hospitals, 3 private hospitals, 1 charity hospital, and 1 military hospital. This descriptive study was based on available data from hospital blood banks in the province of Qazvin. The research instrument was a 2-part questionnaire. The first part was related to demographic characteristics of hospitals and the second part elicited information about blood and blood component wastage. The collected data were then analyzed using descriptive statistic methods and SPSS 11.5. RESULTS: Blood wastage may occur for a number of reasons, including time expiry, wasted imports, blood medically or surgically ordered but not used, stock time expired, hemolysis, or miscellaneous reasons. Data indicated that approximately 77.9% of wasted pack cell units were wasted for the reason of time expiry. Pack cell wastage in hospitals is reported to range from 1.93% to 30.7%. Wastage at all hospitals averaged 9.8% among 30.913 issued blood products. Overall blood and blood product (packed red cells, plasma, platelets, and cryoprecipitate wastage was 3048 units and average total wastage per participant hospital for all blood groups was 254 units per year. CONCLUSION: Blood transfusion is an essential part of patient care. The blood transfusion system has made significant advancements in areas such as donor management, storage of blood, cross-matching, rational use of blood, and distribution. In order to improve the standards of blood banks and the blood transfusion services in Iran, comprehensive standards have been formulated to ensure better quality control in collection, storage, testing, and distribution of blood and its components for the identified major factors

  9. Quality of transfusion products in blood banking.

    Science.gov (United States)

    Franchini, Massimo; Capuzzo, Enrico; Turdo, Rosalia; Glingani, Claudia

    2014-03-01

    The primary goal in transfusion medicine and cellular therapies is to promote high standards of quality and produce ever safer and more efficacious products. The establishment of a transfusion service quality management system, which includes several organizational structures, responsibilities, policies, processes, procedures, and resources, is now mandatory and widely regulated worldwide. In this review, we summarize the current knowledge on the quality system in transfusion medicine as applied to the production of blood components, including red blood cells, platelets, and fresh frozen plasma. PMID:24474089

  10. Cadmium blood concentrations in relation to nutrition.

    Science.gov (United States)

    Krajcovicová-Kudládková, Marica; Ursínyová, Monika; Masánová, Vlasta; Béderová, Alzbeta; Valachovicová, Martina

    2006-09-01

    Cadmium is a toxic element ubiquitous in the environment, which damages biological systems in various ways. The major source of cadmium exposure is food. High cadmium content in the soil leads to high cadmium concentrations in certain plants such as grains (above all surface layers and germs), oil or non-oil seeds, fruit and vegetables. These food commodities are the crucial components of a vegetarian nutrition. Blood cadmium concentrations were measured in two non-smoking population groups: the vegetarian group (n = 80) and the non-vegetarian (control) group of general population on traditional mixed diet (n = 84). The significantly higher blood cadmium content (1.78 +/- 0.22 vs. 0.45 +/- 0.04 microg/l) was measured in vegetarian group. Healthy risk values > 5 microg/l were found in 6 vegetarians vs. no non-vegetarian. The highest cadmium concentration (3.15 +/- 0.77 microg/l) was measured in vegan subgroup (plant food only, n = 10) and that value decreased with increasing animal food consumption (1.75 +/- 0.36 microg/l, lactovegetarian and lactoovovegetarian subgroup/added dairy products and eggs, n = 41/, 1.34 +/- 0.21 microg/I, semivegetarian subgroup /as a previous subgroup and added white meat, n = 291). Risk vegetarians vs. non-risk vegetarians consume significantly higher amounts of whole grain products, grain sprouts and oil seeds. Blood cadmium content is directly influenced by age (r = 0.32, p vegetarianism (r = 0.5, p Vegetarians have significantly higher plasma concentrations of natural antioxidants. The sufficient antioxidative protection against cadmium induced free radical formation in vegetarians may inhibit the harmful effects of greater cadmium intake from plant food. PMID:17152224

  11. High-pressure processing for preservation of blood products

    NARCIS (Netherlands)

    Matser, A.M.; Ven, van der C.; Gouwerok, C.W.N.; Korte, de D.

    2005-01-01

    The possibilities of high pressure as a preservation method for human blood products were evaluated by examining the functional properties of blood fractions, after high-pressure processing at conditions which potentially inactivate micro-organisms and viruses. Blood platelets, red blood cells and b

  12. [Blood donation: Representations and issues associated with blood product collection].

    Science.gov (United States)

    Loquier, B; Zegierman, A; Pelletier, B

    2015-08-01

    In order to answer to its aim of standardised self-sufficiency, the Établissement français du sang (main French national platform for blood donation) needs to know well the donors, what moves them, what motivates them, and the meaning that they give to their action. This knowledge allows the EFS to better understand the different sensitivities among donors, and therefore to improve the strategy regarding loyalty or/and newcomers. In this paper we follow, without attempting to be fully exhaustive, the evolution of the research regarding blood donation. The aim is to highlight the role played by social and historical representations regarding donation in general. In a given community, its norms, and its values influence both the image of donors as well as the meaning attached to the act itself of donation. Moreover, these norms have also influenced the way that research has tried to analyse this topic. The initial studies conducted in this field tried generally to understand the meaning and the symbolism attached to the act of blood donation. Later on, researchers started to focus on the assessment of notions such as generosity, and then solidarity. Nowadays, research is more focused on describing the population that gives blood. They are more scrutinised through their socio-demographic traits (who they are) than through the specific study of how they donate, the reasons behind the decision to act and the notion of satisfaction. PMID:26112917

  13. Radionuclide renogram as a predictor of relative renal blood flow

    International Nuclear Information System (INIS)

    Radionuclide renograms obtained with Tc-99m-DTPA were used to calculate relative renal blood flow in 16 dogs. In 13 of these dogs, relative blood flow ratios (R:L) calculated from the renograms were correlated with ratios measured with an electromagnetic flowmeter or with radiolabeled microspheres. The results indicate that Tc-99m-DTPA renograms can be used to make an accurate prediction of relative renal blood flow

  14. Time-dependent histamine release from stored human blood products

    DEFF Research Database (Denmark)

    Nielsen, Hans Jørgen; Edvardsen, L; Vangsgaard, K; Dybkjaer, E; Skov, P S

    1996-01-01

    Perioperative transfusion of whole blood has been shown to amplify trauma-induced immunosuppression, which could be attenuated by perioperative administration of histamine2 receptor antagonists. Supernatants from different blood products were, therefore, analysed for histamine content during...... full amount of leucocytes, and this may play a significant role in detrimental effects of blood transfusion....

  15. Irradiation of blood products - current status, problems and requirements

    International Nuclear Information System (INIS)

    The irradiation of blood products prior to transfusion is a routine practice in modern medicine. The annihilation of immunocompetent cells in bio products prevents the development of a number of acute and late reactions and consequences. Bone-marrow transplantation is a therapy of especially high risk. The main problems ensue from the toxicity of the preceding treatment, the durable hemopoietic and immune deficiency, the rejection immune reactions and the ability of survival of the malignant cell populations. The irradiation of blood products is of primary importance for the protection from the related with transfusions reaction of the transplant against the host, the so-called Graft-versus-Host-Disease. The optimal irradiation procedure at the present stage of knowledge is considered to be to apply a dose of 30 Gy and a minimal dose of 25 Gy. A number of dosimetric investigations prove the considerable deviations from the nominal dose, as well as other technical problems occur, obviously due to imperfection of the used instrumentation. The construction, principles of operation and a number of technical problems leading to inhomogeneous dose distribution, most often verified by the use of phantoms, are considered in detail for the applied irradiators. The necessity of using standard procedures in dosimetry and of the high quality assurance of blood irradiators has been pointed out. The most recent requirements in this respect have been presented. (authors)

  16. EFSA Panel on Dietetic Products, Nutrition and Allergies (NDA); Scientific Opinion on the substantiation of health claims related to policosanols from sugar cane wax and maintenance of normal blood LDL-cholesterol concentrations (ID 1747, 1748, 1864, 1951, 1954, 4693) and maintenance of normal blood HDL-cholesterol concentrations (ID 1747, 1748, 1864, 1951, 1954, 4693) pursuant to Article 13(1) of Regulation (EC) No 1924/2006

    OpenAIRE

    Tetens, Inge

    2011-01-01

    Following a request from the European Commission, the Panel on Dietetic Products, Nutrition and Allergies was asked to provide a scientific opinion on a list of health claims pursuant to Article 13 of Regulation (EC) No 1924/2006. This opinion addresses the scientific substantiation of health claims in relation to policosanols from sugar cane wax and maintenance of normal blood LDL-cholesterol concentrations and maintenance of normal blood HDL-cholesterol concentrations. The scientific substa...

  17. A rational framework for production decision making in blood establishments.

    Science.gov (United States)

    Ramoa, Augusto; Maia, Salomé; Lourenço, Anália

    2012-01-01

    SAD_BaSe is a blood bank data analysis software, created to assist in the management of blood donations and the blood production chain in blood establishments. In particular, the system keeps track of several collection and production indicators, enables the definition of collection and production strategies, and the measurement of quality indicators required by the Quality Management System regulating the general operation of blood establishments. This paper describes the general scenario of blood establishments and its main requirements in terms of data management and analysis. It presents the architecture of SAD_BaSe and identifies its main contributions. Specifically, it brings forward the generation of customized reports driven by decision making needs and the use of data mining techniques in the analysis of donor suspensions and donation discards. PMID:22829575

  18. Optimizing the use of blood products in trauma care

    OpenAIRE

    Hess, John R.; Hiippala, Seppo

    2005-01-01

    Blood transfusion has been used to treat the injured since the US Civil War. Now, it saves the lives of tens of thousands of injured patients each year. However, not everyone who receives blood benefits, and some recipients are injured by the transfusion itself. Effective blood therapy in trauma management requires an integration of information from diverse sources, including data relating to trauma and blood use epidemiology, medical systems management, and clinical care. Issues of current c...

  19. Federalism and technological change in blood products.

    Science.gov (United States)

    Taylor, Mark Zachary

    2009-12-01

    Recent research has shown how federalism affects health care finance, health care reform, and health policy innovation. The purpose of this article is to extend this research program to study the linkages between federalism and technological change. It does so using comparative case studies spanning five countries to examine innovation and diffusion of two blood technologies-enzyme-linked immunosorbent assays (ELISA blood tests) and heat treatment-in response to the threat to the blood supply posed by HIV during the 1980s. Prior research has produced three contradictory models of the federalism-innovation relationship. This article attempts to resolve these contradictions, posits new hypotheses, and highlights sources of omitted variable bias that have important implications for understanding technological change. The case studies show that overall decentralization, rather than federalism alone, aids technological progress by allowing its supporters to "venue shop" around political resistance. Decentralization also makes the state less vulnerable to capture by status-quo interest groups. Moreover, political decentralization may have a positive effect on technological diffusion, but a far weaker effect on innovation. Thus, prior research that conflates these two effects should be revisited. PMID:20018985

  20. 21 CFR 607.65 - Exemptions for blood product establishments.

    Science.gov (United States)

    2010-04-01

    ... certified under the Clinical Laboratory Improvement Amendments of 1988 (42 U.S.C. 263a) and 42 CFR part 493... the profession of pharmacy including the business of dispensing and selling blood products at...

  1. Is aerobic workload positively related to ambulatory blood pressure?

    DEFF Research Database (Denmark)

    Korshøj, Mette; Clays, Els; Lidegaard, Mark;

    2016-01-01

    PURPOSE: Cardiovascular disease is prevalent among workers with high levels of occupational physical activity. The increased risk may be due to a high relative aerobic workload, possibly leading to increased blood pressure. However, studies investigating the relation between relative aerobic...... workload and ambulatory blood pressure (ABP) are lacking. The aim was to explore the relationship between objectively measured relative aerobic workload and ABP. METHODS: A total of 116 cleaners aged 18-65 years were included after informed consent was obtained. A portable device (Spacelabs 90217) was...

  2. Time-dependent histamine release from stored human blood products

    DEFF Research Database (Denmark)

    Nielsen, Hans Jørgen; Edvardsen, L; Vangsgaard, K;

    1996-01-01

    Perioperative transfusion of whole blood has been shown to amplify trauma-induced immunosuppression, which could be attenuated by perioperative administration of histamine2 receptor antagonists. Supernatants from different blood products were, therefore, analysed for histamine content during...... storage. Whole blood (six units), plasma-reduced whole blood (six units), and plasma- and buffy coat-reduced (saline-adenine-glucose-mannitol) (SAGM) blood (six units) from unpaid healthy donors were stored in the blood bank for 35 days at 4 degrees C. Plasma histamine and total cell-bound histamine...... content at donation, and histamine concentration in samples drawn from the units on days 0, 2, 5, 9, 14, 21, 28 and 35 were analysed with an enzyme-linked immunosorbent assay. Median plasma histamine concentration was 4.8 (range 1.9-14.3) nmol/l (n = 18). Median total cell-bound histamine content was 417...

  3. Relations of blood pressure and head injury to regional cerebral blood flow.

    Science.gov (United States)

    Kisser, Jason E; Allen, Allyssa J; Katzel, Leslie I; Wendell, Carrington R; Siegel, Eliot L; Lefkowitz, David; Waldstein, Shari R

    2016-06-15

    Hypertension confers increased risk for cognitive decline, dementia, and cerebrovascular disease. These associations have been attributed, in part, to cerebral hypoperfusion. Here we posit that relations of higher blood pressure to lower levels of cerebral perfusion may be potentiated by a prior head injury. Participants were 87 community-dwelling older adults - 69% men, 90% white, mean age=66.9years, 27.6% with a history of mild traumatic brain injury (mTBI) defined as a loss of consciousness ≤30min resulting from an injury to the head, and free of major medical (other than hypertension), neurological or psychiatric comorbidities. All engaged in clinical assessment of systolic and diastolic blood pressure (SBP, DBP) and single photon emission computed tomography (SPECT). Computerized coding of the SPECT images yielded relative ratios of blood flow in left and right cortical and select subcortical regions. Cerebellum served as the denominator. Sex-stratified multiple regression analyses, adjusted for age, education, race, alcohol consumption, smoking status, and depressive symptomatology, revealed significant interactions of blood pressure and head injury to cerebral blood flow in men only. Specifically, among men with a history of head injury, higher systolic blood pressure was associated with lower levels of perfusion in the left orbital (β=-3.21, p=0.024) and left dorsolateral (β=-2.61, p=0.042) prefrontal cortex, and left temporal cortex (β=-3.36, p=0.014); higher diastolic blood pressure was marginally associated with lower levels of perfusion in the left dorsolateral prefrontal cortex (β=-2.79, p=0.051). Results indicate that men with a history of head injury may be particularly vulnerable to the impact of higher blood pressure on cerebral perfusion in left anterior cortical regions, thus potentially enhancing risk for adverse brain and neurocognitive outcomes. PMID:27206865

  4. Detection of Epstein-Barr virus in leucoreduced blood products.

    Science.gov (United States)

    Trottier, H; Delage, G; Hu, J; Robitaille, N; Buteau, C; Tucci, M; Lacroix, J; Alfieri, C

    2016-02-01

    This study examined the prevalence of three human herpesviruses (HHV), namely HHV-4 (Epstein-Barr virus/EBV), HHV-6b and HHV-7 in leucoreduced blood products obtained from the Sainte-Justine Hospital blood bank. A total of 100 specimens, including 34 red blood cell concentrates, 33 platelet bags and 33 plasma units, were collected and screened by a sensitive PCR assay using virus-specific primers. Positive units were then retested by quantitative PCR. Of the 100 specimens, one platelet unit tested positive for EBV. PMID:26383177

  5. Prions: Clinical significance, epidemiology and possibilities of transmission by blood and blood products

    Directory of Open Access Journals (Sweden)

    Vojvodić Svetlana

    2002-01-01

    Full Text Available Introduction Prions play a leading role in development of transmissible spongiform encephalopathies (TSE. These infectious particles, smaller than viruses and viroids, consist of special kind of proteins and don't contain nucleic acids. Due to that, they are named defective viruses. Biochemical characteristics Prion protein determined as PrPcs was isolated in infective material of all known prion diseases. This represents an abnormal conformer of PrPc prion protein, normally synthetized in all cells, predominantly in neurons of vertebrates. Pathogenesis of TSE Transmissible spongiform encephalopathies are neurodegenarative diseases whose pathogenesis includes modification of secondary structure of normal glycoprotein - prion protein. In human peripheral blood, PrP are expressed in T and B lymphocytes, NK cells, monocytes, dendritic cells, platelets and plasma. Epidemiological studies Investigations of transmission of prions to experimental animals showed that lymphoid tissues are infectious in the earliest phase of the disease. Experimental transmission of sporadic Creutzfeldt-Jakob disease (CJD by intracerebral inoculation of experimental animals with whole blood buffy coat, platelet concentrates, plasma, early Cohn fractions and albumin preparations, has been reported. A small number of clinical studies reported transmission of CJD by human blood, urine and lymphoid tissues and the role of B lymphocytes in neuroinvasion and transmission of prions. Conclusion Thus, importance of prion diseases in blood transfusion is obvious. Iatrogenic transmission of prion diseases by blood and blood products, can't be neglected. Moreover, it is necessary to find markers in prion diseases as well as new technologies and methodologies in blood transfusion practice to minimize the theoretical risk of transmission of prion diseases by blood and blood products.

  6. Questionnaire-Related Deferrals in Regular Blood Donors in Norway

    Directory of Open Access Journals (Sweden)

    Håkon Reikvam

    2012-01-01

    Full Text Available Voluntary donation is a key issue in transfusion medicine. To ensure the safety of blood transfusions, careful donor selection is important. Although new approaches to blood safety have dramatically reduced the risks for infectious contamination of blood components, the quality and the availability of blood components depend on the willingness to donate and the reliability of the information given by the donors about their own health, including risk behavior. As donors who are deferred by the blood bank will be less motivated to return for donation, it is important to reduce the number of deferrals. The aims of the present study were to investigate the reasons for deferral of registered donors coming to the blood bank for donation, in order to identify areas of importance for donor education—as these deferrals potentially could be avoided by better donor comprehension. Deferral related to testing of donors is not included in this study as these deferrals are dependent on laboratory results and cannot be indentified by questionnaire or interview. Data were collected from all blood donors in a period for 18 months who came for blood donation at a large university hospital in Norway. 1 163 of the 29 787 regular donors, who showed up for donation, were deferred (3.9%. The main reasons were intercurrent illness (n=182 (15.6%, skin ulcers (n=170 (14.6%, and risk behaviour (n=127 (10.9%. In a community, intercurrent illnesses, skin ulcers, and potential risk behavior are the most frequent reasons for deferral of regular donors. Strategized effort on donor education is needed, as “failure to donate” reduces donor motivation.

  7. Use of statistical process control in the production of blood components

    DEFF Research Database (Denmark)

    Magnussen, K.; Quere, S.; Winkel, P.

    2008-01-01

    Introduction of statistical process control in the setting of a small blood centre was tested, both on the regular red blood cell production and specifically to test if a difference was seen in the quality of the platelets produced, when a change was made from a relatively large inexperienced...... an experienced staff with four technologists. We applied statistical process control to examine if time series of quality control values were in statistical control. Leucocyte count in red blood cells was out of statistical control. Platelet concentration and volume of the platelets produced by the...... occasional staff were out of control, which was not the case with the experienced staff. Introduction of control charts to a small blood centre has elucidated the difficulties in controlling the blood production and shown the advantage of using experienced regular component manufacturing staff Udgivelsesdato...

  8. Use of statistical process control in the production of blood components

    DEFF Research Database (Denmark)

    Magnussen, K; Quere, S; Winkel, P

    2008-01-01

    Introduction of statistical process control in the setting of a small blood centre was tested, both on the regular red blood cell production and specifically to test if a difference was seen in the quality of the platelets produced, when a change was made from a relatively large inexperienced...... an experienced staff with four technologists. We applied statistical process control to examine if time series of quality control values were in statistical control. Leucocyte count in red blood cells was out of statistical control. Platelet concentration and volume of the platelets produced by the...... occasional staff were out of control, which was not the case with the experienced staff. Introduction of control charts to a small blood centre has elucidated the difficulties in controlling the blood production and shown the advantage of using experienced regular component manufacturing staff....

  9. Simple technique for measuring relative renal blood flow

    International Nuclear Information System (INIS)

    To determine whether externally monitored early renal uptake of 131I-hippurate is proportional to renal blood flow, the renal uptake of 131-hippurate at 1 to 2 min after injection was compared with the renal accumulation of radioactive carbonized microspheres in dogs. A renal artery catheter equipped with a balloon was used to decrease renal blood flow unilaterally. One minute after the intravenous injection of 100 μCi of 131I-hippurate, about 1 μCi of either 85Sr- or 95Nb-labeled carbon microspheres was injected into the left ventricle. Radioactivity was measured over both kidneys. The total radioactivity within each kidney region of interest was corrected for background and integrated over the 1 to 2 min interval after injection. Thirteen measurements of relative renal blood flow were made for seven dogs. The dogs were then killed and both kidneys were excised and counted for the radioactivity of the microspheres. The 1 to 2-min relative renal uptake of 131I-hippurate correlated well with relative microsphere uptake, suggesting that relative renal blood flow can be simply determined from the external measurements of renal uptake of 131I-hippurate

  10. RELATIVE BLOOD VOLUME: THE MEANS OF INTRADIALYSIS HYPOTENSION PREVENTION

    OpenAIRE

    V. A. Terehov; A. G. Strokov

    2010-01-01

    Intravascular volume preservation is the first choice measure for the prevention of intradialysis hypotension. At present there are devices that allow continuous monitoring of relative blood volume (RBV) changes during haemodialysis (HD). The aim of this research was to investigate (i) the regularity of RBV curve during haemo- dialysis and (ii) the efficacy of some measures for intravascular volume preservation. In patients with hyperhydration RBV curves were monotone; in all cases relation ...

  11. 77 FR 45638 - Blood Products Advisory Committee; Notice of Meeting

    Science.gov (United States)

    2012-08-01

    ... meeting will also be Web cast. The Web cast will be available at the following links: On September 20, 2012, Blood Products Advisory Committee Day 1, http://fda.yorkcast.com/webcast/Viewer/?peid.../webcast/Viewer/?peid=8effe88a1e834779b4932f882b67e3391d . Contact Person: Bryan Emery or...

  12. 77 FR 4567 - Blood Products Advisory Committee; Notice of Meeting

    Science.gov (United States)

    2012-01-30

    ..., the meeting will also be Web cast. The Blood Products Advisory Committee Web cast will be available at http://fda.yorkcast.com/webcast/Viewer/?peid=11253ea88a9041e5a91883236f342bfc1d . Contact Person: Bryan.... Therefore, you should always check the Agency's Web site and call the appropriate advisory committee...

  13. 77 FR 20643 - Blood Products Advisory Committee; Notice of Meeting

    Science.gov (United States)

    2012-04-05

    ... unable to attend in person, the meeting will also be Web cast. The Web cast will be available at the following links. Blood Products Advisory Committee Web Cast Link May 15 http://fda.yorkcast.com/webcast/Viewer/?peid=ba104b31fe4c4c099568bacda9a4e5401d . May 16...

  14. Effects of a hospital-wide introduction of a massive transfusion protocol on blood product ratio and blood product waste

    Directory of Open Access Journals (Sweden)

    Kirsten Balvers

    2015-01-01

    Full Text Available Background: Massive transfusion protocols (MTPs are increasingly used in the transfusion practice and are developed to provide the standardized and early delivery of blood products and procoagulant agents and to supply the transfusion of blood products in a well-balanced ratio. Aim: The aim of this study was to investigate the effect of a hospital-wide introduction of an MTP on blood product ratio and a waste of blood products. Materials and Methods: Retrospective analysis was performed to compare the transfusion practice in massive bleeding patients before and after the introduction of an MTP and between the use of an MTP and transfusion off-protocol. Massive bleeding was defined as an administration of ≥5 units of red blood cells (RBCs within 12 h. Results: Of 547 massively transfused patients, 192 patients were included in the pre-MTP period and 355 patients in the MTP period. The ratio of RBC to fresh frozen plasma (FFP and the platelets transfused shifted significantly toward 1:1:1 in the MTP period (P = 0.012. This was mainly caused by a shift in RBC: FFP ratio (P = 0.014. An increase in the waste of blood products was observed, most notably FFPs (P = 0.026. Extending the storage time after thawing reduced the waste of FFPs from 11% to 4%. Conclusion: Hospital-wide introduction of an MTP is an adequate way to achieve a well-balanced transfusion ratio of 1:1:1. This comes at the cost of an increase in the waste of FFPs, which is lowered after extending the duration of storage time after thawing.

  15. A computer planning model for blood platelet production and distribution.

    Science.gov (United States)

    Sirelson, V; Brodheim, E

    1991-08-01

    We consider a class of policies for stocking hospital blood banks with units of random donor platelet concentrate ('Platelets') based upon scheduled daily deliveries from a regional blood center to replenish the platelet inventory to a fixed 'base stock' level. The measures of interest are the 'shortage rate' (the proportion of days for which the on-hand inventory at the hospital blood bank is insufficient to meet the demand) and the 'outdate rate' (the proportion of total units shipped which are not transfused within the usable life span of 5 days). Our principal results give a predictive model which relates the base stock level to the shortage rate and outdate rate. Our model uses only the mean daily demand as a parameter. It provides a basis to unify the results from other studies which have demonstrated improvements in platelet inventory management in particular hospitals and blood centers. PMID:1752123

  16. EFSA Panel on Dietetic Products, Nutrition and Allergies (NDA); Scientific Opinion on the substantiation of health claims related to folate and maintenance of normal blood pressure (ID 176) pursuant to Article 13(1) of Regulation (EC) No 1924/2006

    DEFF Research Database (Denmark)

    Tetens, Inge

    Following a request from the European Commission, the Panel on Dietetic Products, Nutrition and Allergies was asked to provide a scientific opinion on a list of health claims pursuant to Article 13 of Regulation (EC) No 1924/2006. This opinion addresses the scientific substantiation of health...... claims in relation to folate and maintenance of normal blood pressure. The scientific substantiation is based on the information provided by the Member States in the consolidated list of Article 13 health claims and references that EFSA has received from Member States or directly from stakeholders. The...... that the claimed effect relates to the maintenance of normal blood pressure. The Panel considers that maintenance of normal blood pressure is a beneficial physiological effect. In weighing the evidence, the Panel took into account that no human intervention studies from which conclusions could be drawn...

  17. EFSA Panel on Dietetic Products, Nutrition and Allergies (NDA); Scientific Opinion on the substantiation of a health claim related to vitamin K2 and contribution to the normal function of the heart and blood vessels (ID 125, further assessment) pursuant to Article 13(1) of Regulation (EC) No 1924/2006

    OpenAIRE

    Tetens, Inge

    2012-01-01

    Following a request from the European Commission, pursuant to Article 13 of Regulation (EC) No 1924/2006, the Panel on Dietetic Products, Nutrition and Allergies was asked to provide a scientific opinion on a health claim related to vitamin K2 and contribution to the normal function of the heart and blood vessels. The food constituent that is the subject of the claim, vitamin K2, is sufficiently characterised. The claimed effect, contribution to the normal function of the heart and blood vess...

  18. Acquired immunodeficiency syndrome associated with blood-product transfusions

    Energy Technology Data Exchange (ETDEWEB)

    Jett, J.R.; Kuritsky, J.N.; Katzmann, J.A.; Homburger, H.A.

    1983-11-01

    A 53-year-old white man had fever, malaise, and dyspnea on exertion. His chest roentgenogram was normal, but pulmonary function tests showed impaired diffusion capacity and a gallium scan showed marked uptake in the lungs. Results of an open-lung biopsy documented Pneumocystis carinii pneumonia. Immunologic test results were consistent with the acquired immunodeficiency syndrome. The patient denied having homosexual contact or using intravenous drugs. Twenty-nine months before the diagnosis of pneumocystis pneumonia was made, the patient had had 16 transfusions of whole blood, platelets, and fresh-frozen plasma during coronary artery bypass surgery at another medical center. This patient is not a member of any currently recognized high-risk group and is believed to have contracted the acquired immunodeficiency syndrome from blood and blood-product transfusions.

  19. Acquired immunodeficiency syndrome associated with blood-product transfusions

    International Nuclear Information System (INIS)

    A 53-year-old white man had fever, malaise, and dyspnea on exertion. His chest roentgenogram was normal, but pulmonary function tests showed impaired diffusion capacity and a gallium scan showed marked uptake in the lungs. Results of an open-lung biopsy documented Pneumocystis carinii pneumonia. Immunologic test results were consistent with the acquired immunodeficiency syndrome. The patient denied having homosexual contact or using intravenous drugs. Twenty-nine months before the diagnosis of pneumocystis pneumonia was made, the patient had had 16 transfusions of whole blood, platelets, and fresh-frozen plasma during coronary artery bypass surgery at another medical center. This patient is not a member of any currently recognized high-risk group and is believed to have contracted the acquired immunodeficiency syndrome from blood and blood-product transfusions

  20. Blood pressure variability in relation to outcome in the International Database of Ambulatory blood pressure in relation to Cardiovascular Outcome

    DEFF Research Database (Denmark)

    Stolarz-Skrzypek, Katarzyna; Thijs, Lutgarde; Richart, Tom;

    2010-01-01

    Ambulatory blood pressure (BP) monitoring provides information not only on the BP level but also on the diurnal changes in BP. In the present review, we summarized the main findings of the International Database on Ambulatory BP in relation to Cardiovascular Outcome (IDACO) with regard to risk.......1%. In conclusion, the IDACO observations support the concept that BP variability adds to risk stratification, but above all highlight that 24-h ambulatory BP level remains the main predictor to be considered in clinical practice....

  1. Relation of urinary calcium and magnesium excretion to blood pressure

    DEFF Research Database (Denmark)

    Kesteloot, Hugo; Tzoulaki, Ioanna; Brown, Ian J;

    2011-01-01

    Data indicate an inverse association between dietary calcium and magnesium intakes and blood pressure (BP); however, much less is known about associations between urinary calcium and magnesium excretion and BP in general populations. The authors assessed the relation of BP to 24-hour excretion...... of calcium and magnesium in 2 cross-sectional studies. The International Study of Macro- and Micro-Nutrients and Blood Pressure (INTERMAP) comprised 4,679 persons aged 40-59 years from 17 population samples in China, Japan, the United Kingdom, and the United States, and the International Cooperative Study......) of higher urinary calcium excretion (associations were smaller for diastolic BP) in INTERMAP. Qualitatively similar associations were observed in INTERSALT analyses. Associations between magnesium excretion and BP were small and nonsignificant for most of the models examined. The present data suggest...

  2. Splanchnic blood flow and hepatic glucose production in exercising humans

    DEFF Research Database (Denmark)

    Bergeron, R; Kjaer, M; Simonsen, L;

    2001-01-01

    The study examined the implication of the renin-angiotensin system (RAS) in regulation of splanchnic blood flow and glucose production in exercising humans. Subjects cycled for 40 min at 50% maximal O(2) consumption (VO(2 max)) followed by 30 min at 70% VO(2 max) either with [angiotensin-converti......The study examined the implication of the renin-angiotensin system (RAS) in regulation of splanchnic blood flow and glucose production in exercising humans. Subjects cycled for 40 min at 50% maximal O(2) consumption (VO(2 max)) followed by 30 min at 70% VO(2 max) either with [angiotensin......-converting enzyme (ACE) blockade] or without (control) administration of the ACE inhibitor enalapril (10 mg iv). Splanchnic blood flow was estimated by indocyanine green, and splanchnic substrate exchange was determined by the arteriohepatic venous difference. Exercise led to an approximately 20-fold increase (P ...-blockade group vs. the control group, hormones, metabolites, VO(2), and RER followed the same pattern of changes in ACE-blockade and control groups during exercise. Splanchnic blood flow (at rest: 1.67 +/- 0.12, ACE blockade; 1.59 +/- 0.18 l/min, control) decreased during moderate exercise (0.78 +/- 0.07, ACE...

  3. EFSA Panel on Dietetic Products, Nutrition and Allergies (NDA); Scientific Opinion on the substantiation of health claims related to isoleucine-proline-proline (IPP) and valine-proline-proline (VPP) and maintenance of normal blood pressure (ID 661, 1831, 1832, 2891, further assessment) pursuant to Article 13(1) of Regulation (EC) No 1924/2006 (further reassessment)

    OpenAIRE

    Tetens, Inge

    2012-01-01

    Following a request from the European Commission, pursuant to Article 13.1 of Regulation (EC) No 1924/2006, the Panel on Dietetic Products, Nutrition and Allergies was asked to provide a scientific opinion on a health claim related to isoleucine-proline-proline (IPP) and valine-proline-proline (VPP) and maintenance of normal blood pressure. The food constituent that is the subject of the claim, the tripeptides isoleucine-proline-proline (IPP) and valine-proline-proline (VPP), is sufficiently ...

  4. EFSA Panel on Dietetic Products, Nutrition and Allergies (NDA); Scientific Opinion on the substantiation of health claims related to acetic acid and maintenance of normal blood pressure (ID 1447) pursuant to Article 13(1) of Regulation (EC) No 1924/2006

    DEFF Research Database (Denmark)

    Tetens, Inge

    Following a request from the European Commission, the Panel on Dietetic Products, Nutrition and Allergies was asked to provide a scientific opinion on a list of health claims pursuant to Article 13 of Regulation (EC) No 1924/2006. This opinion addresses the scientific substantiation of health...... claims in relation to acetic acid and maintenance of normal blood pressure. The scientific substantiation is based on the information provided by the Member States in the consolidated list of Article 13 health claims and references that EFSA has received from Member States or directly from stakeholders...... Panel considers that maintenance of normal blood pressure is a beneficial physiological effect. In weighing the evidence, the Panel took into account that although one animal study showed an effect of acetic acid administration on systolic blood pressure, results from two human intervention studies are...

  5. EFSA NDA Panel (EFSA Panel on Dietetic Products, Nutrition and Allergies), 2014. Scientific Opinion on the substantiation of a health claim related to iron and contribution to normal formation of haemoglobin and red blood cells pursuant to Article 14 of Regulation (EC) No 1924/2006

    DEFF Research Database (Denmark)

    Tetens, Inge

    Following an application from Specialised Nutrition Europe (formerly IDACE), submitted pursuant to Article 14 of Regulation (EC) No 1924/2006 via the Competent Authority of France, the EFSA Panel on Dietetic Products, Nutrition and Allergies (NDA) was asked to deliver an opinion on the scientific...... substantiation of a health claim related to iron and contribution to normal formation of haemoglobin and red blood cells. The food constituent, iron, which is the subject of the health claim, is sufficiently characterised. Contribution to normal formation of haemoglobin and red blood cells is a beneficial...... physiological effect for infants and young children. A claim on iron and contribution to normal formation of haemoglobin and red blood cells in the general population has already been assessed by the Panel with a favourable outcome. The Panel considers that the role of iron in normal formation of haemoglobin...

  6. 76 FR 39405 - Blood Products Advisory Committee; Notice of Meeting

    Science.gov (United States)

    2011-07-06

    ...-8900. For those unable to attend in person, the meeting will also be Web cast. The Web cast will be.../Viewer/?peid=b6ce0d080a594ddf9d362a0b1815b4491d . Blood Products Advisory Committee Day 2: http://fda.yorkcast.com/webcast/Viewer/?peid=68d4630cf50847c5aaec06b1720f205f1d . Contact Person: Bryan Emery...

  7. EFSA Panel on Dietetic Products, Nutrition and Allergies (NDA); Scientific Opinion on the substantiation of health claims related to policosanols from sugar cane wax and maintenance of normal blood LDL-cholesterol concentrations (ID 1747, 1748, 1864, 1951, 1954, 4693) and maintenance of normal, blood HDL-cholesterol concentrations (ID 1747, 1748, 1864, 1951, 1954, 4693) pursuant to Article 13(1) of Regulation (EC) No 1924/2006

    DEFF Research Database (Denmark)

    Tetens, Inge

    claims in relation to policosanols from sugar cane wax and maintenance of normal blood LDL-cholesterol concentrations and maintenance of normal blood HDL-cholesterol concentrations. The scientific substantiation is based on the information provided by the Member States in the consolidated list of Article...... 13 health claims and references that EFSA has received from Member States or directly from stakeholders. The food constituent that is the subject of the health claims is policosanols from sugar cane wax. The Panel considers that policosanols from sugar cane wax are sufficiently characterised.......Following a request from the European Commission, the Panel on Dietetic Products, Nutrition and Allergies was asked to provide a scientific opinion on a list of health claims pursuant to Article 13 of Regulation (EC) No 1924/2006. This opinion addresses the scientific substantiation of health...

  8. Blood money: Bayer's inventory of HIV-contaminated blood products and third world hemophiliacs.

    Science.gov (United States)

    McHenry, Leemon; Khoshnood, Mellad

    2014-01-01

    This article presents an overlooked case of research misconduct and violations of basic principles of medical and business ethics. When Bayer's Cutter Laboratories realized that their blood products, Factor VIII and IX or antihemophiliac factor (AHF), were contaminated with human immunodeficiency virus (HIV), the financial investment in the product was considered too high to destroy the inventory. Cutter misrepresented the results of its own research and sold the contaminated AHF to overseas markets in Asia and Latin America without the precaution of heat treating the product recommended for eliminating the risk. As a consequence, hemophiliacs who infused the HIV-contaminated Factor VIII and IX tested positive for HIV and developed AIDS. PMID:24785997

  9. Questionnaire-Related Deferrals in Regular Blood Donors in Norway

    OpenAIRE

    Håkon Reikvam; Kjersti Svendheim; Anne S. Røsvik; Tor Hervig

    2012-01-01

    Voluntary donation is a key issue in transfusion medicine. To ensure the safety of blood transfusions, careful donor selection is important. Although new approaches to blood safety have dramatically reduced the risks for infectious contamination of blood components, the quality and the availability of blood components depend on the willingness to donate and the reliability of the information given by the donors about their own health, including risk behavior. As donors who are deferred by the...

  10. Individual variability in human blood metabolites identifies age-related differences

    Science.gov (United States)

    Murakami, Itsuo; Takada, Junko; Kondoh, Hiroshi; Yanagida, Mitsuhiro

    2016-01-01

    Metabolites present in human blood document individual physiological states influenced by genetic, epigenetic, and lifestyle factors. Using high-resolution liquid chromatography-mass spectrometry (LC-MS), we performed nontargeted, quantitative metabolomics analysis in blood of 15 young (29 ± 4 y of age) and 15 elderly (81 ± 7 y of age) individuals. Coefficients of variation (CV = SD/mean) were obtained for 126 blood metabolites of all 30 donors. Fifty-five RBC-enriched metabolites, for which metabolomics studies have been scarce, are highlighted here. We found 14 blood compounds that show remarkable age-related increases or decreases; they include 1,5-anhydroglucitol, dimethyl-guanosine, acetyl-carnosine, carnosine, ophthalmic acid, UDP-acetyl-glucosamine, N-acetyl-arginine, N6-acetyl-lysine, pantothenate, citrulline, leucine, isoleucine, NAD+, and NADP+. Six of them are RBC-enriched, suggesting that RBC metabolomics is highly valuable for human aging research. Age differences are partly explained by a decrease in antioxidant production or increasing inefficiency of urea metabolism among the elderly. Pearson’s coefficients demonstrated that some age-related compounds are correlated, suggesting that aging affects them concomitantly. Although our CV values are mostly consistent with those CVs previously published, we here report previously unidentified CVs of 51 blood compounds. Compounds having moderate to high CV values (0.4–2.5) are often modified. Compounds having low CV values, such as ATP and glutathione, may be related to various diseases because their concentrations are strictly controlled, and changes in them would compromise health. Thus, human blood is a rich source of information about individual metabolic differences. PMID:27036001

  11. Individual variability in human blood metabolites identifies age-related differences.

    Science.gov (United States)

    Chaleckis, Romanas; Murakami, Itsuo; Takada, Junko; Kondoh, Hiroshi; Yanagida, Mitsuhiro

    2016-04-19

    Metabolites present in human blood document individual physiological states influenced by genetic, epigenetic, and lifestyle factors. Using high-resolution liquid chromatography-mass spectrometry (LC-MS), we performed nontargeted, quantitative metabolomics analysis in blood of 15 young (29 ± 4 y of age) and 15 elderly (81 ± 7 y of age) individuals. Coefficients of variation (CV = SD/mean) were obtained for 126 blood metabolites of all 30 donors. Fifty-five RBC-enriched metabolites, for which metabolomics studies have been scarce, are highlighted here. We found 14 blood compounds that show remarkable age-related increases or decreases; they include 1,5-anhydroglucitol, dimethyl-guanosine, acetyl-carnosine, carnosine, ophthalmic acid, UDP-acetyl-glucosamine,N-acetyl-arginine,N6-acetyl-lysine, pantothenate, citrulline, leucine, isoleucine, NAD(+), and NADP(+) Six of them are RBC-enriched, suggesting that RBC metabolomics is highly valuable for human aging research. Age differences are partly explained by a decrease in antioxidant production or increasing inefficiency of urea metabolism among the elderly. Pearson's coefficients demonstrated that some age-related compounds are correlated, suggesting that aging affects them concomitantly. Although our CV values are mostly consistent with those CVs previously published, we here report previously unidentified CVs of 51 blood compounds. Compounds having moderate to high CV values (0.4-2.5) are often modified. Compounds having low CV values, such as ATP and glutathione, may be related to various diseases because their concentrations are strictly controlled, and changes in them would compromise health. Thus, human blood is a rich source of information about individual metabolic differences. PMID:27036001

  12. Performance Related Pay and Labour Productivity

    OpenAIRE

    Gielen, Anne; Kerkhofs, Marcel J. M.; van Ours, Jan C.

    2006-01-01

    This paper uses information from a panel of Dutch firms to investigate the labour productivity effects of performance related pay (PRP). We find that PRP increases labour productivity at the firm level with about 9%.

  13. Single-tube-genotyping of gastric cancer related SNPs by directly using whole blood and paper-dried blood as starting materials

    Institute of Scientific and Technical Information of China (English)

    Huan Huang; Ying Bu; Guo-Hua Zhou

    2006-01-01

    AIM: To demonstrate an inexpensive method for typing gastric cancer related single nucleotide polymorphisms (SNPs) using whole blood or paper-dried blood as starting materials.METHODS: PCR amplification is directly carried out from the whole blood or paper-dried blood sample without any DNA extraction step. Before PCR, a blood sample, four primers, and all of biological reagents necessary for PCR were added at a time; After PCR, the amplified products were directly separated by slab gel electrophoresis or microchip CE without any purification. SNP typing was performed by tetra-primer PCR with two inner primers specific to each allele and two outer primers defining the length of allele-specific amplicons. Genotypes were directly discriminated by the size of amplicons specific to each allele, thereby avoiding any post-PCR process.RESULTS: Using a special PCR buffer, inhibitory substances in blood (including the anticoagulant in blood) and filter paper were effectively suppressed;a "true" single-tube-genotyping is thus realized. We successfully determined genotypes IL-1B-511 and IL-1B-31 polymorphisms at the gene IL-1B by using whole-blood and paper-dried blood samples as starting materials respectively. The method is so sensitive that 0.5-1.0 μL of blood sample is enough to give a satisfactory typing results. The genotyping results were confirmed by RFLP-PCR using purified genome DNA,indicating that amplification specificity was not affected by inhibitory components (including coagulants) in blood or filter paper.CONCLUSION: Compared with SNP typing methods based on purified DNA, the proposed method is laborsaving, simple, inexpensive, and less cross-contaminated.It is promising to use this method to type other SNPs.

  14. Dextrose in the banked blood products does not seem to affect the blood glucose levels in patients undergoing liver transplantation

    OpenAIRE

    Cheng, Kwok-Wai; Chen, Chao-Long; Cheng, Yu-Fan; Tseng, Chia-Chih; Wang, Chih-Hsien; Chen, Yaw-Sen; Wang, Chih-Chi; Huang, Tung-Liang; Eng, Hock-Liew; Chiu, King-Wah; Wang, Shih-Hor; Lin, Chih-Che; Lin, Tsan-Shiun; Liu, Yueh-Wei; Jawan, Bruno

    2005-01-01

    AIM: Hyperglycemia commonly seen in liver transplantation (LT) has often been attributed to the dextrose in the storage solution of blood transfusion products. The purpose of the study is to compare the changes of the blood glucose levels in transfused and non-transfused patients during LT.

  15. The average unit production cost of blood in Zimbabwe from a provider's perspective

    NARCIS (Netherlands)

    Mafirakureva, N.; Nyoni, H.; Chikwereti, R.; Khoza, S.; Mvere, D.A.; Emmanuel, J.C.; Postma, M.J.; Van Hulst, M.

    2014-01-01

    Background/Case Studies: Blood utilization and blood transfusion costs are generally perceived to be increasing at a time when healthcare budgets continue being constricted. There is a paucity of published data on the production costs of blood and the costs of blood transfusion in sub-Saharan Africa

  16. The relative nutritive value of irradiated spray-dried blood powder and heat-sterilized blood meal as measured in combination with whey protein

    International Nuclear Information System (INIS)

    A method of processing blood meal in which nutritive value of the protein is preserved is described, since appreciable losses occur in the nutritive value of the protein when prepared by heat sterilization with drying at atmospheric pressure in steam jacketed vessels. Blood was spray dried and irradiated at an intensity of 10 kGy. Collectively the heat of spray drying and irradiation was effective in killing both the virus plaque-forming units and the bacteria, thus producing a commercially acceptable sterile product of higher nutritive value. The relative nutritive values (RNV) of 50:50 protein were 0,56 for whey protein concentrate plus heat-sterilized blood meal and 0.90 for whey protein concentrate plus irradiated spray-dried blood powder. Whey protein concentrate used as a control has a RNV of 1,0

  17. RELATIVE BLOOD VOLUME: THE MEANS OF INTRADIALYSIS HYPOTENSION PREVENTION

    Directory of Open Access Journals (Sweden)

    V. A. Terehov

    2010-06-01

    Full Text Available Intravascular volume preservation is the first choice measure for the prevention of intradialysis hypotension. At present there are devices that allow continuous monitoring of relative blood volume (RBV changes during haemodialysis (HD. The aim of this research was to investigate (i the regularity of RBV curve during haemo- dialysis and (ii the efficacy of some measures for intravascular volume preservation. In patients with hyperhydration RBV curves were monotone; in all cases relation RBV/ ultrafiltration volume (UF did not exceed 2,5%/L. In stable patients the RBV curve was immutable in the course of years. Patients with high RBV/UF ratio (>6%/L formed a high risk group. In these patients stability of RBV was more im- portant and more useful. Isolated UF did not decrease RBV drop, as well as haemodiafiltration online. Albumin administration allows to decrease RBV/UF ratio. After bolus of hypertonic dextrose solution RBV increased for about half of hour. In patients with acute renal injury RBV monitoring was far from reliability in many cases. 

  18. EFSA Panel on Dietetic Products, Nutrition and Allergies (NDA); Scientific Opinion on the substantiation of a health claim related to OptiEFAX™ and maintenance of normal blood LDL-cholesterol concentrations pursuant to Article 13(5) of Regulation (EC) No 1924/2006

    OpenAIRE

    Tetens, Inge

    2012-01-01

    Following an application from Nutrilinks Sarl, submitted for authorisation of a claim pursuant to Article 13(5) of Regulation (EC) No 1924/2006 via the Competent Authority of Belgium, the Panel on Dietetic Products, Nutrition and Allergies (NDA) was asked to deliver an opinion on the scientific substantiation of a health claim related to OptiEFAX™ and maintenance of normal blood LDL-cholesterol concentrations. The food that is the subject of the health claim, OptiEFAX™, which is standardised ...

  19. EFSA Panel on Dietetic Products, Nutrition and Allergies (NDA); Scientific Opinion on the substantiation of a health claim related to OptiEFAX™ and maintenance of normal blood concentrations of triglycerides pursuant to Article 13(5) of Regulation (EC) No 1924/2006

    OpenAIRE

    Tetens, Inge

    2012-01-01

    Following an application from Nutrilinks Sarl, submitted for authorisation of a health claim pursuant to Article 13(5) of Regulation (EC) No 1924/2006 via the Competent Authority of Belgium, the Panel on Dietetic Products, Nutrition and Allergies (NDA) was asked to deliver an opinion on the scientific substantiation of a health claim related to OptiEFAX™ and maintenance of normal blood concentrations of triglycerides. The food that is the subject of the health claim, OptiEFAX™, which is stand...

  20. EFSA Panel on Dietetic Products, Nutrition and Allergies (NDA); Scientific Opinion on the substantiation of a health claim related to OptiEFAX™ and maintenance of normal blood HDL-cholesterol concentrations pursuant to Article 13(5) of Regulation (EC) No 1924/2006

    OpenAIRE

    Tetens, Inge

    2012-01-01

    Following an application from Nutrilinks Sarl, submitted for authorisation of a health claim pursuant to Article 13(5) of Regulation (EC) No 1924/2006 via the Competent Authority of Belgium, the Panel on Dietetic Products, Nutrition and Allergies (NDA) was asked to deliver an opinion on the scientific substantiation of a health claim related to OptiEFAX™ and maintenance of normal blood HDL-cholesterol concentrations. The food that is the subject of the health claim, OptiEFAX™, which is standa...

  1. EFSA Panel on Dietetic Products, Nutrition and Allergies (NDA); Scientific Opinion on the substantiation of a health claim related to 3 g/day plant stanols as plant stanol esters and lowering blood LDL-cholesterol and reduced risk of (coronary) heart disease pursuant to Article 14 of Regulation (EC) No 1924/2006

    OpenAIRE

    Tetens, Inge

    2012-01-01

    Following an application from Raisio Nutrition Ltd, submitted pursuant to Article 14 of Regulation (EC) No 1924/2006 via the Competent Authority of Finland, the Panel on Dietetic Products, Nutrition and Allergies was asked to deliver an opinion on the scientific substantiation of a health claim related to 3 g/day plant stanols as plant stanol esters per day and lowering blood LDL-cholesterol by 12 % and reduced risk of (coronary) heart disease. The applicant has further requested that the min...

  2. Estimated daily salt intake in relation to blood pressure and blood lipids

    DEFF Research Database (Denmark)

    Thuesen, Betina H; Toft, Ulla; Buhelt, Lone P;

    2015-01-01

    BACKGROUND: Excessive salt intake causes increased blood pressure which is considered the leading risk for premature death. One major challenge when evaluating associations between daily salt intake and markers of non-communicable diseases is that a high daily salt intake correlates with obesity......, which is also a well described risk factor for poor cardiometabolic outcome. The aim of this study was to evaluate the relationship of estimated daily salt intake with blood pressure and blood lipids and to investigate the effect of taking different measures of obesity into account. METHODS: We included...... estimated 24-hour sodium excretion with blood pressure and blood lipids were evaluated by linear regression models. RESULTS: The daily mean estimated intake of salt was 10.80 g and 7.52 g among men and women, respectively. Daily salt intake was significantly associated with blood pressure (β-estimates 1...

  3. Heritability of blood pressure traits and the genetic contribution to blood pressure variance explained by four blood-pressure-related genes.

    NARCIS (Netherlands)

    Rijn, M.J. van; Schut, A.F.; Aulchenko, Y.S.; Deinum, J.; Sayed-Tabatabaei, F.A.; Yazdanpanah, M.; Isaacs, A.; Axenovich, T.I.; Zorkoltseva, I.V.; Zillikens, M.C.; Pols, H.A.; Witteman, J.C.; Oostra, B.A.; Duijn, C.M. van

    2007-01-01

    OBJECTIVE: To study the heritability of four blood pressure traits and the proportion of variance explained by four blood-pressure-related genes. METHODS: All participants are members of an extended pedigree from a Dutch genetically isolated population. Heritability and genetic correlations of systo

  4. Distribution of di(2-ethylhexyl) phthalate and products in blood and blood components.

    OpenAIRE

    G. Rock; Labow, R S; Tocchi, M

    1986-01-01

    In order to impart flexibility, plastic medical devices incorporate liquid plasticizers into their structure. Data from several laboratories, including ours, have shown that these compounds leach from blood bags and tubing during collection of blood, storage of various blood components and during kidney dialysis and cell and plasma apheresis procedures. After the plasticizer di(2-ethylhexyl) phthalate leaches from poly(vinyl chloride) blood packs, it is converted by a plasma enzyme to a more ...

  5. Shear stress related blood damage in laminar couette flow.

    Science.gov (United States)

    Paul, Reinhard; Apel, Jörn; Klaus, Sebastian; Schügner, Frank; Schwindke, Peter; Reul, Helmut

    2003-06-01

    Artificial organs within the blood stream are generally associated with flow-induced blood damage, particularly hemolysis of red blood cells. These damaging effects are known to be dependent on shear forces and exposure times. The determination of a correlation between these flow-dependent properties and actual hemolysis is the subject of this study. For this purpose, a Couette device has been developed. A fluid seal based on fluorocarbon is used to separate blood from secondary external damage effects. The shear rate within the gap is controlled by the rotational speed of the inner cylinder, and the exposure time by the amount of blood that is axially pumped through the device per given time. Blood damage is quantified by the index of hemolysis (IH), which is calculated from photometric plasma hemoglobin measurements. Experiments are conducted at exposure times from texp=25 - 1250 ms and shear rates ranging from tau=30 up to 450 Pa ensuring Taylor-vortex free flow characteristics. Blood damage is remarkably low over a broad range of shear rates and exposure times. However, a significant increase in blood damage can be observed for shear stresses of tau>or= 425 Pa and exposure times of texp>or= 620 ms. Maximum hemolysis within the investigated range is IH=3.5%. The results indicate generally lower blood damage than reported in earlier studies with comparable devices, and the measurements clearly indicate a rather abrupt (i.e., critical levels of shear stresses and exposure times) than gradual increase in hemolysis, at least for the investigated range of shear rates and exposure times. PMID:12780506

  6. Effective RES blood flow changes in children with homozygous β-thalassemia in relation to blood transfusion

    International Nuclear Information System (INIS)

    Denatured radioiodinated human serum albumin (DHA) clearance studies at a dose of 1 mg/kg body wt., were carried out in 16 thalassemic children, prior to and 7-10 days following blood transfusion, to investigate changes of the effective RES blood flow which might accompany the posttransfusion spleen size diminution. A statistically significant increase (P<0.001) of the DHA plasma clearance rate was observed 7-10 days following blood transfusion denoting an increase of the blood flow to the effective RES while at the same time the spleen diminished in size. It is suggested that changes in the effective RES blood flow in these patients are directly related to changes in the intrasplenic circulatory capacity. (orig.)

  7. Infectivity of blood products from donors with occult hepatitis B virus infection

    DEFF Research Database (Denmark)

    Allain, Jean-Pierre; Mihaljevic, Ivanka; Gonzalez-Fraile, Maria Isabel;

    2013-01-01

    Occult hepatitis B virus (HBV) infection (OBI) is identified in 1:1000 to 1:50,000 European blood donations. This study intended to determine the infectivity of blood products from OBI donors.......Occult hepatitis B virus (HBV) infection (OBI) is identified in 1:1000 to 1:50,000 European blood donations. This study intended to determine the infectivity of blood products from OBI donors....

  8. Patient Safety with Blood Products Administration Using Wireless and Bar-Code Technology

    OpenAIRE

    Porcella, Aleta; Walker, Kristy

    2005-01-01

    Supported by a grant from the Agency for Healthcare Research and Quality, a University of Iowa Hospitals and Clinics interdisciplinary research team created an online data-capture-response tool utilizing wireless mobile devices and bar code technology to track and improve blood products administration process. The tool captures 1) sample collection, 2) sample arrival in the blood bank, 3) blood product dispense from blood bank, and 4) administration. At each step, the scanne...

  9. Biomarker Analysis of Stored Blood Products: Emphasis on Pre-Analytical Issues

    Directory of Open Access Journals (Sweden)

    Niels Lion

    2010-11-01

    Full Text Available Millions of blood products are transfused every year; many lives are thus directly concerned by transfusion. The three main labile blood products used in transfusion are erythrocyte concentrates, platelet concentrates and fresh frozen plasma. Each of these products has to be stored according to its particular components. However, during storage, modifications or degradation of those components may occur, and are known as storage lesions. Thus, biomarker discovery of in vivo blood aging as well as in vitro labile blood products storage lesions is of high interest for the transfusion medicine community. Pre-analytical issues are of major importance in analyzing the various blood products during storage conditions as well as according to various protocols that are currently used in blood banks for their preparations. This paper will review key elements that have to be taken into account in the context of proteomic-based biomarker discovery applied to blood banking.

  10. Biomarker analysis of stored blood products: emphasis on pre-analytical issues.

    Science.gov (United States)

    Delobel, Julien; Rubin, Olivier; Prudent, Michel; Crettaz, David; Tissot, Jean-Daniel; Lion, Niels

    2010-01-01

    Millions of blood products are transfused every year; many lives are thus directly concerned by transfusion. The three main labile blood products used in transfusion are erythrocyte concentrates, platelet concentrates and fresh frozen plasma. Each of these products has to be stored according to its particular components. However, during storage, modifications or degradation of those components may occur, and are known as storage lesions. Thus, biomarker discovery of in vivo blood aging as well as in vitro labile blood products storage lesions is of high interest for the transfusion medicine community. Pre-analytical issues are of major importance in analyzing the various blood products during storage conditions as well as according to various protocols that are currently used in blood banks for their preparations. This paper will review key elements that have to be taken into account in the context of proteomic-based biomarker discovery applied to blood banking. PMID:21151459

  11. Greening Non-Product-Related Procurement

    DEFF Research Database (Denmark)

    Mosgaard, Mette; Riisgaard, Henrik; Huulgaard, Rikke Dorothea

    2013-01-01

    well described in literature: Green supply chain management in the business-to-business area, green public procurement, and, finally green private consumption. The latest strategy with special emphasis on non-product-related procurement makes sense from a macro-level perspective: the small...... investigation of the drivers of and barriers to the greening of non-product-related procurement. The research shows that, as a main challenge, the clear benefits achieved at the macro-level are almost invisible at the micro-level, where the management priorities tend to focus on the primary, customer......Green procurement in Denmark is developing with a new strategy with special emphasis on non-product-related procurement; i.e., all products that are not directly transformed into the product produced by the company, for example cleaning detergents and office supplies. Three earlier strategies are...

  12. Body mass index relates to blood pressure among adults

    Directory of Open Access Journals (Sweden)

    Suman Dua

    2014-01-01

    Full Text Available Background: The blood pressure and anthropometric measurements are important for evaluating the health of children, adolescents as well as adults. Aim: The aim is to study the blood pressure and body dimensions and to find out the prevalence of overweight/obesity and hypertension among adults. Materials and Methods: A cross-sectional study was conducted of all the people belonging to the Punjabi community, residing in Roshanara area and Jaina building in Delhi, for the past 20 years and aged 18-50 years. The men were engaged in transport business and women were mainly housewives. Results: Mean values of all the measurements, that is, height, weight, upper arm circumference, pulse rate, systolic blood pressure (SBP, and diastolic blood pressure (DBP were higher among males as compared with females, except skinfold thicknesses. Body mass index (BMI and fat percentage was found to be higher among females as compared with males. There was a significant positive correlation between BMI, fat percentage, and blood pressure both SBP as well as DBP. Odds ratio showed that overweight/obese subjects were more likely to have hypertension than those with normal BMI. Conclusion: Prevalence of prehypertension among overweight/obese suggested an early clinical detection of prehypertension and intervention including life style modification, particularly weight management.

  13. Blood biochemistry evaluation in horses hyperimmune sera production

    Energy Technology Data Exchange (ETDEWEB)

    Baptista, Tatyana S. [Instituto de Pesquisas Energeticas e Nucleares (IPEN/CNEN-SP), Sao Paulo, SP (Brazil); Instituto Butantan, Sao Paulo, SP (Brazil); E-mail: proplasma@butantan.gov.br; Zamboni, Cibele [Instituto de Pesquisas Energeticas e Nucleares IPEN/CNEN-SP, Sao Paulo, SP (Brazil); E-mail: czamboni@ipen.br; Marcelino, Jose R.; Freitas, Monica G. [Instituto Butantan, Sao Paulo, SP (Brazil); E-mail: marcelino@butantan.gov.br; mfreitas@uol.com

    2007-07-01

    In the present study the biochemical values for Cl, K and Na in whole blood from health equine used for immunization process were determined using nuclear methodology. The results were compared with human being whole blood estimation permitting a discussion about the similarities between the medium values and the reference intervals taken {+-} 1 and {+-} 2 SD (Standard Deviation). We intend to use these data as interval value for Cl, K and Na in whole blood of equines to perform clinical practical. (author)

  14. Talking about relations: Factors influencing the production of relational descriptions

    OpenAIRE

    Adriana eBaltaretu; Emiel eKrahmer; Alfons eMaes; Carel evan Wijk

    2016-01-01

    In a production experiment (Experiment 1) and an acceptability rating one (Experiment 2), we assessed two factors, spatial position and salience, which may influence the production of relational descriptions (such as the ball between the man and the drawer). In Experiment 1, speakers were asked to refer unambiguously to a target object (a ball). In Experiment 1a, we addressed the role of spatial position, more specifically if speakers mention the entity positioned leftmost in the scene as (fi...

  15. Age and gender related differences in aortic blood flow

    DEFF Research Database (Denmark)

    Traberg, Marie Sand; Pedersen, Mads Møller; Hemmsen, Martin Christian; Lönn, Lars; Henneberg, Kaj-Åge; Jensen, Jørgen Arendt

    2012-01-01

    The abdominal aorta (AA) is predisposed to development of abdominal aneurysms (AAA), a focal dilatation of the artery with fatal consequences if left untreated. The blood flow patterns in the AA is thought to play an important role in the development of AAA. The purpose of this work is to investi...

  16. Pharmaceutical protein production by yeast: towards production of human blood proteins by microbial fermentation

    DEFF Research Database (Denmark)

    Martínez, José L; Liu, Lifang; Petranovic, Dina;

    2012-01-01

    Since the approval of recombinant insulin from Escherichia coli for its clinical use in the early 1980s, the amount of recombinant pharmaceutical proteins obtained by microbial fermentations has significantly increased. The recent advances in genomics together with high throughput analysis...... techniques (the so-called—omics approaches) and integrative approaches (systems biology) allow the development of novel microbial cell factories as valuable platforms for large scale production of therapeutic proteins. This review summarizes the main achievements and the current situation in the field of...... recombinant therapeutics using yeast Saccharomyces cerevisiae as a model platform, and discusses the future potential of this platform for production of blood proteins and substitutes....

  17. Current state of methodological and decisions for radiation treatment of blood, its components and products

    Directory of Open Access Journals (Sweden)

    Gordeev A.V.

    2014-12-01

    Full Text Available This article presents currently used blood transfusion media — components and blood products, therapeutic effects, reactions and complications of blood transfusion, use of radiation treatment for blood transfusion fluids. There had been discussed in detail the practice of radiation processing of blood components and for the prevention of reaction "graft versus host" and studies of plasma radiation treatment for its infectious safety. There was presented the current state of techniques and technical solutions of radiation treatment of transfusion-transmissible environments. There were also considered an alternative to radiation treatment of blood.

  18. Changes in Blood Parameters and Coagulation-Related Gene Expression in Pregnant Rats

    OpenAIRE

    Urasoko, Yoshinaka; He, Xi Jun; Ebata, Tomonori; KINOSHITA, YUICHI; Kobayashi, Junichi; Mochizuki, Masahiro; Ikeya, Masamichi

    2009-01-01

    The present study examined changes in maternal blood parameters, particularly those related to blood coagulation, as well as alterations in blood coagulation-related gene expression in the liver during gestation in rats. Fibrinogen concentration and platelet count increased as pregnancy progressed whereas prothrombin time and overall activity of vitamin-K–dependent coagulation factors decreased before delivery, suggesting a physiologic response to prevent prolonged bleeding at parturition. Co...

  19. Blood Lipids and other Related Risk Factors of stroke

    Institute of Scientific and Technical Information of China (English)

    Liu YAND; Hiu Lu

    2000-01-01

    Abstract To assess the ralationship between incidence of stroke and blood lipids, apolipoprolcin (apo) , 219 patients with stroke(cerebral hemorrhage 87, cerebral infarction 132) , diagnosed with the aid of CT, were examined. The levels of serum total cholesterol, triglycerioes, apo-A, apo-B were calculatea. 160 cases with no disorder in metabolism of blood lipids or other disease of meurologic system were taken as centrol group. The results zhowed that the ratio of high density lipoprotein cholesterol and low clensity lipoprotein cholesterol was iower than.that of control group(p<0. 05) . In the patients group, cases with diabetes, hypentension coronary heart disease or onesity, the levels of blood liprds. triglyceride and cholesterol were higher than those vithout above mentioned disease The condusion is, that there is certain reletionship between the contents of bliid lipids. lipoprotein and it sulqraction HDL2, HDL3 and cerebrovasculor cisease. The radis of high density lipoprotein and low density lipoprolein is the important factor in predicting of stroke

  20. EFSA Panel on Dietetic Products, Nutrition and Allergies (NDA); Scientific Opinion on the substantiation of health claims related to monacolin K from red yeast rice and maintenance of normal blood LDL-cholesterol concentrations (ID 1648, 1700) pursuant to Article 13(1) of Regulation (EC) No 1924/2006

    DEFF Research Database (Denmark)

    Tetens, Inge

    Following a request from the European Commission, the Panel on Dietetic Products, Nutrition and Allergies was asked to provide a scientific opinion on a list of health claims pursuant to Article 13 of Regulation (EC) No 1924/2006. This opinion addresses the scientific substantiation of health cla......, the Panel refers to the Summary of Product Characteristics of lovastatin-containing medicinal products available on the EU market.......Following a request from the European Commission, the Panel on Dietetic Products, Nutrition and Allergies was asked to provide a scientific opinion on a list of health claims pursuant to Article 13 of Regulation (EC) No 1924/2006. This opinion addresses the scientific substantiation of health...... claims in relation to monacolin K from red yeast rice and maintenance of normal blood LDL-cholesterol concentrations. The scientific substantiation is based on the information provided by the Member States in the consolidated list of Article 13 health claims and references that EFSA has received from...

  1. Carbon monoxide concentration in donated blood : relation to cigarette smoking and other sources

    OpenAIRE

    Åberg, Anna-Maja; Nilsson Sojka, Birgitta; Winsö, Ola; Abrahamsson, Pernilla; Johansson, Göran; Larsson, Jan Erik

    2009-01-01

    BACKGROUND: Carbon monoxide (CO) is normally present in the human body due to endogenous production of CO. CO can also be inhaled by exposure to external sources such as cigarette smoke, car exhaust, and fire. The purpose of this study was to investigate CO concentrations in blood from 410 blood donors at the blood center in Umea, Sweden. To further evaluate the effects of cigarette smoking on CO concentrations, the elimination time for CO was examined in six volunteer smokers after a smoked ...

  2. FREE RADICAL-RELATED DISEASES: THE PREDICTIVE VALUE OF BIOMARKERS IN THE UMBILICAL CORD BLOOD

    Directory of Open Access Journals (Sweden)

    S. Perrone

    2013-12-01

    Full Text Available Despite recent advances in preterm newborns healthcare, the incidence of neonatal pathologies and disabilities still remain unacceptable high. The deficiency of antioxidant systems and the high free radicals (FRs production may cause several neonatal diseases, such as Retinopathy of Prematurity (ROP, Bronchopulmonary Dysplasia (BPD, Necrotizing Enterocolitis (NEC, Patent Ductus Arteriosus (PDA, Periventricular Leukomalacia (PVL and Intraventricular Hemorrhage (IVH, representing facets of the ‘Free Radical-Related Diseases’ (FRD. The aim of this study is to verify the association between FRD and blood levels of reliable oxidative stress (OS biomarkers in preterm newborns. We enrolled 178 preterm newborns born consecutively at the General Hospital “Santa Maria alle Scotte” in Siena, between 23 and 34 weeks (30,36±2.97 of gestational age, with birth-weight from 430 to 2890 grams (1453±593. After birth, we evaluated in the cord blood the markers of potential risk of OS (Non Protein-Bound Iron, NPBI and the markers of FR damage (Total Hydroperoxides, TH; Advanced Oxidation Protein Products, AOPP. For each newborn, we assessed the presence or absence of the following diseases, considering as FRD the presence of one at least: ROP, BPD, NEC, PDA, PVL, IVH. The univariate logistic regression showed a significant association between FRD and OS related markers. Risk assessment of FRD was higher in newborns with higher values of each OS marker: respectively TH (OR=1.013, p=0,000, AOPP (OR=1.017, p=0,036, NPBI (OR=1.077, p=0.039. Perinatal OS exposure is linked to the main diseases of prematurity. The evaluation of OS biomarkers in preterm newborns through the analysis of umbilical cord blood, can be useful and predictive for early identification of infants at risk for FRD in order to devise appropriate and timely prevention and treating strategies.

  3. EFSA NDA Panel (EFSA Panel on Dietetic Products, Nutrition and Allergies), 2015. Scientific Opinion on the substantiation of a health claim related to Symbiosal® and lowering of blood pressure and reduced risk of hypertension pursuant to Article 14 of Regulation (EC) No 1924/2006

    DEFF Research Database (Denmark)

    Tetens, Inge

    scientific substantiation of a health claim related to Symbiosal® and lowering of blood pressure and reduced risk of hypertension. The Panel considers that the food, Symbiosal®, which is the subject of the health claim, and the food, table salt, which Symbiosal® should replace, are sufficiently characterised....... Lowering of blood pressure is a beneficial physiological effect for people who want to lower their blood pressure. Increased blood pressure is a risk factor for hypertension. In weighing the evidence, the Panel took into account that one human study with methodological limitations showed a decrease in...

  4. Alternative commutation relations, star products and tomography

    OpenAIRE

    Man'ko, Olga V.; V. I. Man'ko; Marmo, G.

    2001-01-01

    Invertible maps from operators of quantum obvservables onto functions of c-number arguments and their associative products are first assessed. Different types of maps like Weyl-Wigner-Stratonovich map and s-ordered quasidistribution are discussed. The recently introduced symplectic tomography map of observables (tomograms) related to the Heisenberg-Weyl group is shown to belong to the standard framework of the maps from quantum observables onto the c-number functions. The star-product for sym...

  5. A relation between blood pressure and stiffness of joints and skin

    NARCIS (Netherlands)

    Uiterwaal, CSPM; Grobbee, DE; Sakkers, RJB; Helders, PJM; Bank, RA; Engelbert, RHH

    2003-01-01

    Background. Blood pressure, particularly pulse pressure, is associated with arterial wall stiffness, but little is known about its relation to stiffness of other parts of the body. We examined the extent to which blood pressure levels in young healthy children are related to stiffness of various tis

  6. Bacterial glycosidases for the production of universal red blood cells

    DEFF Research Database (Denmark)

    Liu, Qiyong P; Sulzenbacher, Gerlind; Yuan, Huaiping;

    2007-01-01

    Enzymatic removal of blood group ABO antigens to develop universal red blood cells (RBCs) was a pioneering vision originally proposed more than 25 years ago. Although the feasibility of this approach was demonstrated in clinical trials for group B RBCs, a major obstacle in translating this...... alpha-N-acetylgalactosaminidase family reveals an unusual catalytic mechanism involving NAD+. The enzymatic conversion processes we describe hold promise for achieving the goal of producing universal RBCs, which would improve the blood supply while enhancing the safety of clinical transfusions....

  7. EFSA Panel on Dietetic Products, Nutrition and Allergies (NDA); Scientific Opinion on the substantiation of a health claim related to isolated soy protein and reduction of blood LDL-cholesterol concentrations pursuant to Article 14 of Regulation (EC) No 1924/2006

    DEFF Research Database (Denmark)

    Tetens, Inge

    on Dietetic Products, Nutrition and Allergies was asked to deliver an opinion on the scientific substantiation of a health claim related to isolated soy protein (ISP) and reduction of blood LDL-cholesterol concentrations, referring to disease risk reduction. The food constituent that is the subject......Following an application from the European Natural Soyfood Manufacturers Association (ENSA), the European Vegetable Protein Federation (EUVEPRO) and the Soya Protein Association (SPA), submitted pursuant to Article 14 of Regulation (EC) No 1924/2006 via the Competent Authority of Belgium, the Panel...... into account that most of these RCTs were at high risk of bias, that differences in the results obtained between trials appear unrelated to the dose of ISP used, to sample size or to study duration, and that the evidence provided in support of a possible mechanism was not convincing. A cause and effect...

  8. EFSA Panel on Dietetic Products, Nutrition and Allergies (NDA); Scientific Opinion on the substantiation of health claims related to foods with reduced amounts of sodium and maintenance of normal blood pressure (ID 336, 705, 1148, 1178, 1185, 1420) pursuant to Article 13(1) of Regulation (EC) No

    DEFF Research Database (Denmark)

    Tetens, Inge

    Following a request from the European Commission, the Panel on Dietetic Products, Nutrition and Allergies was asked to provide a scientific opinion on a list of health claims pursuant to Article 13 of Regulation (EC) No 1924/2006. This opinion addresses the scientific substantiation of health...... claims in relation to foods with reduced amounts of sodium and maintenance of normal blood pressure. The scientific substantiation is based on the information provided by the Member States in the consolidated list of Article 13 health claims and references that EFSA has received from Member States or...... directly from stakeholders. The food constituents that are the subjects of the health claims are “sodium/salt”, “low sodium/salt and/or increased potassium”, “food reduced/low in/free of sodium/salt and/or increased potassium”, “breads with salt content of...

  9. Complications related to blood donation: A multicenter study of the prevalence and influencing factors in voluntary blood donation camps in Karnataka, India

    OpenAIRE

    Rajat Kumar Agarwal; Sundar Periyavan; Rakesh Dhanya; Parmar, Lalith G.; Amit Sedai; Kumari Ankita; Arpit Vaish; Ritesh Sharma; Prabha Gowda

    2016-01-01

    Introduction: Complications associated with blood donation significantly lower odds of subsequent donations. The aim of the study is to assess the prevalence of complications related to blood donation, identify the influencing factors, and come up with suggestions for minimizing discomfort to donors and making outdoor voluntary blood donation camps safer. Materials and Methods: This study covered 181 blood donation camps organized by Sankalp India Foundation where 16 blood banks participated ...

  10. Cord blood cytokines are modulated by maternal farming activities and consumption of farm dairy products during pregnancy: the PASTURE Study.

    NARCIS (Netherlands)

    Pfefferle, P.I.; Buchele, G.; Blumer, N.; Roponen, M.; Ege, M.J.; Krauss-Etschmann, S.; Genuneit, J.; Hyvarinen, A.; Hirvonen, M.R.; Lauener, R.; Pekkanen, J.; Riedler, J.; Dalphin, J.C.; Brunekreef, B.; Braun-Fahrlander, C.; von Mutius, E.

    2010-01-01

    BACKGROUND: Traditional farming represents a unique model situation to investigate the relationship of early-life farm-related exposure and allergy protection. OBJECTIVES: To investigate associations between maternal farm exposures and cytokine production in cord blood (CB) mononuclear cells in a pr

  11. Blood Donors on Medication - an Approach to Minimize Drug Burden for Recipients of Blood Products and to Limit Deferral of Donors.

    Science.gov (United States)

    Becker, Christian D K; Stichtenoth, Dirk O; Wichmann, Michael G; Schaefer, Christof; Szinicz, Ladislaus

    2009-01-01

    BACKGROUND: Blood products derived from donors on medication can contain drugs which might pose a risk for the recipients or influence the quality of the product itself. MATERIAL AND METHODS: To judge the eligibility of blood donors on medication, 4 drug classes have been formed with respect to their pharmacological properties, and blood products have been divided in accordance with their single-donor plasma contents. RESULTS: For drugs with dose-dependent pharmacodynamics, no deferral periods are necessary for donation of blood products containing less than 50 ml single-donor plasma for application to adults. Waiting periods of t(max) + 5 t(1/2) were calculated for the other blood products. Teratogenic drugs do not require special considerations (exception: retinoids, thalidomide and lenalidomide, dutasteride or finasteride with waiting periods for all blood products). A deferral period of t(max) + 24 t(1/2) is proposed for every blood product from blood donors on genotoxic drugs. Drugs without systemic effects can be neglected. Irreversible inhibitors of platelet function cause a 10-day waiting period if production of platelet concentrates is intended. CONCLUSION: Donors on medication are allowed to donate blood for blood products containing less than 50 ml plasma of a single donor, like red blood cell concentrates, for the use in adults without deferral periods, except those taking retinoids, thalidomide, lenalidomide, dutasteride, finasteride, or genotoxic drugs. PMID:20823991

  12. Chromatographic techniques for petroleum and related products

    Energy Technology Data Exchange (ETDEWEB)

    Barman, B.N.; Cebolla, V.L.; Membrado, L. [Equilon Enterprises LLC, Houston, TX (USA)

    2000-07-01

    Recent developments in chromatographic techniques for the separation and quantitative characterization of petroleum and related products are highlighted. Specifically, scope, applicability and versatility of individual techniques such as gas chromatography, liquid chromatography, supercritical fluid chromatography, thin-layer chromatography, and size-exclusion chromatography are discussed in some detail.

  13. Products and relations in symplectic Floer homology

    OpenAIRE

    Betz, Marty; Rade, Johan

    1995-01-01

    We give a construction of a version of the Gromov-Witten classes, Q: H_*(J) -> HF_*(M) otimes ... otimes HF^*(M), within the context of symplectic Floer (co)homology. In particular, this gives a functorial approach to products and relations in symplectic Floer (co)homology.

  14. Dextrose in the banked blood products does not seem to affect the blood glucose levels in patients undergoing liver transplantation

    Institute of Scientific and Technical Information of China (English)

    Kwok-Wai Cheng; King-Wah Chiu; Shih-Hor Wang; Chih-Che Lin; Tsan-Shiun Lin; Yueh-Wei Liu; Bruno Jawan; Chao-Long Chen; Yu-Fan Cheng; Chia-chih Tseng; Chih-Hsien Wang; Yaw-Sen Chen; Chih-Chi Wang; Tung Liang Huang; Hock-Liew Eng

    2005-01-01

    AIM: Hyperglycemia commonly seen in liver transplantation (LT) has often been attributed to the dextrose in the storage solution of blood transfusion products. The purpose of the study is to compare the changes of the blood glucose levels in transfused and non-transfused patients during LT. METHODS: A retrospective study on 60 biliary pediatric patients and 16 adult patients undergoing LT was carried out. Transfused pediatric patients were included in Group Ⅰ (GI), those not transfused in Group Ⅱ (GⅡ). Twelve adult patients were not given transfusion and assigned to Group Ⅲ (GⅢ); whereas, four adult patients who received massive transfusion were assigned to Group Ⅳ (GⅣ). The blood glucose levels, volume of blood transfused, and the volume of crystalloid infused were recorded, compared and analyzed. RESULTS: Results showed that the changes in bloodglucose levels during LT for both non-transfused and minimally transfused pediatric groups and non-transfused and massively-transfused adult groups were almost the same. CONCLUSION: We conclude that blood transfusion does not cause significant changes in the blood glucose levels inthis study.

  15. Individual variability in human blood metabolites identifies age-related differences

    OpenAIRE

    Chaleckis, Romanas; MURAKAMI, Itsuo; Takada, Junko; Kondoh, Hiroshi; Yanagida, Mitsuhiro

    2016-01-01

    Human blood provides a rich source of information about metabolites that reflects individual differences in health, disease, diet, and lifestyle. The coefficient of variation for human blood metabolites enriched in red blood cells or plasma was quantified after careful preparation. We identified 14 age-related metabolites. Metabolites that decline strikingly in the elderly include antioxidants and compounds involved in high physical activity, including carnosine, UDP-acetyl-glucosamine, ophth...

  16. Age-related memory impairments due to reduced blood glucose responses to epinephrine

    OpenAIRE

    Morris, Ken A.; Chang, Qing; Mohler, Eric G.; Gold, Paul E.

    2009-01-01

    Increases in blood glucose levels are an important component of the mechanisms by which epinephrine enhances memory formation. The present experiments addressed the hypothesis that a dysfunction in the blood glucose response to circulating epinephrine contributes to age-related memory impairments. Doses of epinephrine and glucagon that significantly increased blood glucose levels in young adult rats were far less effective at doing so in two-year-old rats. In young rats, epinephrine and gluco...

  17. Bacterial glycosidases for the production of universal red blood cells.

    Science.gov (United States)

    Liu, Qiyong P; Sulzenbacher, Gerlind; Yuan, Huaiping; Bennett, Eric P; Pietz, Greg; Saunders, Kristen; Spence, Jean; Nudelman, Edward; Levery, Steven B; White, Thayer; Neveu, John M; Lane, William S; Bourne, Yves; Olsson, Martin L; Henrissat, Bernard; Clausen, Henrik

    2007-04-01

    Enzymatic removal of blood group ABO antigens to develop universal red blood cells (RBCs) was a pioneering vision originally proposed more than 25 years ago. Although the feasibility of this approach was demonstrated in clinical trials for group B RBCs, a major obstacle in translating this technology to clinical practice has been the lack of efficient glycosidase enzymes. Here we report two bacterial glycosidase gene families that provide enzymes capable of efficient removal of A and B antigens at neutral pH with low consumption of recombinant enzymes. The crystal structure of a member of the alpha-N-acetylgalactosaminidase family reveals an unusual catalytic mechanism involving NAD+. The enzymatic conversion processes we describe hold promise for achieving the goal of producing universal RBCs, which would improve the blood supply while enhancing the safety of clinical transfusions. PMID:17401360

  18. Ferritin blood test

    Science.gov (United States)

    Serum ferritin level ... The amount of ferritin in the blood (serum ferritin level) is directly related to the amount of iron stored in your body. Iron is important for red blood cell production. Your doctor ...

  19. A study on dietary habits, health related lifestyle, blood cadmium and lead levels of college students

    OpenAIRE

    Shin, Nari; Hyun, Whajin; Lee, Hongmie; Ro, Mansoo; Song, Kyunghee

    2012-01-01

    This study was performed in order to investigate dietary habits, health related lifestyle and blood cadmium and lead levels in female college students. 80 college students (43 males and 37 females) participated in the survey questionnaires. Body weight and height, blood pressure, and body composition were measured. The systolic blood pressure of male and female students were 128.9 ± 13.9 and 109.8 ± 12.0, respectively. The diastolic blood pressure of male and female students were 77.1 ± 10.3 ...

  20. Particle Production in 5-Dimensional Cosmological Relativity

    Science.gov (United States)

    Gemelli, Gianluca

    2006-12-01

    The 5-dimensional extension of cosmological special and general relativity is considered. In this framework it is possible to define a 5-dimensional perfect fluid stress-energy tensor and to unify the equations of perfect hydrodynamics in a single 5-dimensional tensor conservation law. This picture in principle permits to interpret particle production phenomena as cosmological effects, in the spirit of open system cosmology.

  1. EFSA Panel on Dietetic Products, Nutrition and Allergies (NDA); Scientific Opinion on the substantiation of a health claim related to OptiEFAX™ and maintenance of normal blood LDL-cholesterol concentrations pursuant to Article 13(5) of Regulation (EC) No 1924/2006

    DEFF Research Database (Denmark)

    Tetens, Inge

    substantiation of a health claim related to OptiEFAX™ and maintenance of normal blood LDL-cholesterol concentrations. The food that is the subject of the health claim, OptiEFAX™, which is standardised pure krill oil, is sufficiently characterised in relation to the claimed effect. The claimed effect, maintenance...

  2. EFSA Panel on Dietetic Products, Nutrition and Allergies (NDA); Scientific Opinion on the substantiation of a health claim related to OptiEFAX™ and maintenance of normal blood HDL-cholesterol concentrations pursuant to Article 13(5) of Regulation (EC) No 1924/2006

    DEFF Research Database (Denmark)

    Tetens, Inge

    scientific substantiation of a health claim related to OptiEFAX™ and maintenance of normal blood HDL-cholesterol concentrations. The food that is the subject of the health claim, OptiEFAX™, which is standardised pure krill oil, is sufficiently characterised in relation to the claimed effect. The claimed...

  3. EFSA Panel on Dietetic Products, Nutrition and Allergies (NDA); Scientific Opinion on the substantiation of a health claim related to OptiEFAX™ and maintenance of normal blood concentrations of triglycerides pursuant to Article 13(5) of Regulation (EC) No 1924/2006

    DEFF Research Database (Denmark)

    Tetens, Inge

    scientific substantiation of a health claim related to OptiEFAX™ and maintenance of normal blood concentrations of triglycerides. The food that is the subject of the health claim, OptiEFAX™, which is standardised pure krill oil, is sufficiently characterised in relation to the claimed effect. The claimed...

  4. Blood-brain barrier transport of butanol and water relative to N-isopropyl-p-iodoamphetamine as the internal reference

    International Nuclear Information System (INIS)

    The literature regarding the blood--brain barrier (BBB) transport of butanol is conflicting as studies report both incomplete and complete extraction of butanol by the brain. In this work the BBB transport of both [14C]butanol and [3H]water was studied using the carotid injection technique in conscious and in ketamine- or pentobarbital-anesthetized rats employing N-isopropyl-p-[125I]iodoamphetamine ([125I]IMP) as the internal reference and as a fluid microsphere. The three isotopes (3H, 125I, 14C) were conveniently counted simultaneously in a liquid scintillation spectrometer. IMP is essentially completely sequestered by the brain for at least 1 min in conscious rats and for 2 min in anesthetized animals. Butanol extraction by rat forebrain is not flow limited but ranges between 77 +/- 1 and 87 +/- 1% for the three conditions. The permeability-surface area product/cerebral blood flow ratio of butanol and water in rat forebrain remains relatively constant, despite a twofold increase in cerebral blood flow in conscious relative to pentobarbital-anesthetized rats. The absence of an inverse relationship between flow and butanol or water extraction is consistent with capillary recruitment being the principal mechanism underlying changes in cerebral blood flow in anesthesia. The diffusion restriction of BBB transport of butanol in some regions, but not in others, necessitates a careful regional analysis of BBB permeability to butanol prior to usage of this compound as a cerebral blood flow marker

  5. EFSA Panel on Dietetic Products, Nutrition and Allergies (NDA); Scientific Opinion on the substantiation of a health claim related to 3 g/day plant sterols/stanols and lowering blood LDL-cholesterol and reduced risk of (coronary) heart disease pursuant to Article 19 of Regulation (EC) No 1924/2006

    DEFF Research Database (Denmark)

    Tetens, Inge

    ) No 376/2010 (yellow fat spreads, dairy products, mayonnaise and salad dressings) have a similar efficacy on blood LDL-cholesterol lowering, that plant sterols and stanol esters at a daily intake of 3 g (range 2.6 g to 3.4 g) plant sterols/stanols in matrices approved by Regulation (EC) No 376...

  6. Evaluation of a dispersion medium for the irradiation of blood products in a electron linear accelerator

    International Nuclear Information System (INIS)

    The use of bags of rice as dispersion medium in irradiation of blood products for medical use is less effective than the immersion from the point of view dosimetric water. However, given the wide range of dose values for a valid result and once subjected to quality control procedure, the method provides the necessary work for the irradiation of blood products in a linear electron accelerator. (Author)

  7. Homocysteine induces production of monocyte chemoattractant protein-1 and interleukin-8 in cultured human whole blood

    Institute of Scientific and Technical Information of China (English)

    Xiao-kun ZENG; Daniel G REMICK; Xian WANG

    2004-01-01

    AIM: To investigate whether increased plasma L-homocysteine (Hcy) level could promote monocyte chemoattractant protein-1 (MCP-1) and interleukin-8 (IL-8) in cultured whole blood. METHODS: Human whole blood or different type of peripheral blood cells from health volunteers were incubated with Hcy and/or the inhibitors. MCP- 1 and IL-8 level were measured by ELISA assay. RESULTS: Hcy 10-1000 μmol/L induced production of MCP-1 and IL-8 in cultured human whole blood (P<0.05). The major cellular source of these chemokines comed from monocytes.Meanwhile,Hcy also promoted the upregulation of MPO level even at the 10 μmol/L in the cultured whole blood.secretion in cultured human whole blood, especially in monocytes via oxidative stress mechanism.

  8. Production of nitric oxide by peripheral blood mononuclear cells from the Florida manatee, Trichechus manatus latirostris.

    Science.gov (United States)

    Walsh, Catherine J; Stuckey, Joyce E; Cox, Heather; Smith, Brett; Funke, Christina; Stott, Jeff; Colle, Clarence; Gaspard, Joseph; Manire, Charles A

    2007-08-15

    Florida manatees (Trichechus manatus latirostris) are exposed to many conditions in their habitat that may adversely impact health and impair immune function in this endangered species. In an effort to increase the current knowledge base regarding the manatee immune system, the production of an important reactive nitrogen intermediate, nitric oxide (NO), by manatee peripheral blood mononuclear cells (PBMC) was investigated. PBMC from healthy captive manatees were stimulated with LPS, IFN-gamma, or TNF-alpha, either alone or in various combinations, with NO production assessed after 24, 48, 72, and 96 h of culture. NO production in response to LPS stimulation was significantly greater after 48, 72, or 96 h of culture compared to NO production after 24h of culture. A specific inhibitor of inducible nitric oxide synthase (iNOS), L-NIL (L-N(6)-(1-iminoethyl)lysine), significantly decreased NO production by LPS-stimulated manatee PBMC. Manatee specific oligonucleotide primers for iNOS were designed to measure expression of relative amounts of mRNA in LPS-stimulated manatee PBMC from captive manatees. NO production by PBMC from manatees exposed to red tide toxins was analyzed, with significantly greater NO production by both unstimulated and LPS stimulated PBMC from red tide exposed compared with healthy captive or cold-stress manatees. Free-ranging manatees produced significantly lower amounts of nitric oxide compared to either captive or red tide rescued manatees. Results presented in this paper contribute to the current understanding of manatee immune function and represent the first report of nitric oxide production in the immune system of a marine mammal. PMID:17614139

  9. Framework for product knowledge and product related knowledge which supports product modelling for mass customization

    DEFF Research Database (Denmark)

    Riis, Jesper; Hansen, Benjamin Loer; Hvam, Lars

    2003-01-01

    The article presents a framework for product knowledge and product related knowledge which can be used to support the product modelling process which is needed for developing IT systems. These IT systems are important tools for many companies when they aim at achieving mass customization and...... personalization. The framework for product knowledge and product related knowledge is based on the following theories: axiomatic design, technical systems, theory of domains, theory of structuring, theory of properties and the framework for the content of product and product related models. The framework is built...... on experience from product modelling projects in several companies. Among them for example companies manufacturing electronic switchboards, spray dryer systems and air conditioning equipment. The framework is divided into three views: the product knowledge view, the life phase system view and the...

  10. High glucose impairs superoxide production from isolated blood neutrophils

    DEFF Research Database (Denmark)

    Perner, A; Nielsen, S E; Rask-Madsen, J

    2003-01-01

    Superoxide (O(2)(-)), a key antimicrobial agent in phagocytes, is produced by the activity of NADPH oxidase. High glucose concentrations may, however, impair the production of O(2)(-) through inhibition of glucose-6-phosphate dehydrogenase (G6PD), which catalyzes the formation of NADPH. This stud...... measured the acute effects of high glucose or the G6PD inhibitor dehydroepiandrosterone (DHEA) on the production of O(2)(-) from isolated human neutrophils....

  11. Evaluation of sedentary women’s ambulatory blood pressure and its relation to muscle strength

    Directory of Open Access Journals (Sweden)

    Ramires Alsamir Tibana

    2012-09-01

    Full Text Available Objective: To compare the ambulatory blood pressure in women with different values of relative muscle strength. Methods: Data from 21 (aged 33.8±8.0 years sedentary women from Vila Telebrasília was collected during the period of November 2010 to July 2011. The volunteers were submitted to the evaluation of the handgrip strength and ambulatory monitoring of blood pressure (AMBP for a 72-hour period. Following the evaluation of handgrip strength to determine the absolute muscle strength, an adjustment in the body mass was made, in order to determine the relative muscle strength. Based on the relative value of muscular strength, the sample was divided into tertiles to compare systolic, diastolic and mean blood pressure during the periods of 24 hours, daytime and night-time, by using an one-way ANOVA, followed by Bonferroni test when appropriate, with a significance level of p<0.05. Results: Significant differences were found for systolic blood pressure between tertile 1 (99.3±12.2 and tertile 3 (106.8±11.1 in the night-time (P<0.05. Values of mean blood pressure were also significantly different between tertile 1 (70.2±6.3 and tertile 3 (80.3 ± 8.8 in the night-time (p<0,05. Conclusion: Women with higher relative muscle strength present lower values of blood pressure during night-time

  12. Association between religiousness and blood donation among Brazilian postgraduate students from health-related areas

    Directory of Open Access Journals (Sweden)

    Edson Zangiacomi Martinez

    2014-06-01

    Full Text Available OBJECTIVE: The aim of this study was to examine the association between religiousness and blood donation among postgraduate students.METHODS: The Portuguese-language version of the Duke University Religion Index was administered to a sample of 226 Brazilian students with ages ranging from 22 to 55 years. All study participants had completed undergraduate courses in health-related areas.RESULTS: In the present study, 23.5% of the students were regular donors. Organizational religiousness was found to be associated with attitudes related to blood donation. This study also shows evidence that regular blood donors have a higher intrinsic religiousness than subjects who donate only once and do not return.CONCLUSION: This study shows that the attitudes concerning blood donation may have some association with religiosity.

  13. Relation of Whole Blood Carboxyhemoglobin Concentration to Ambient Carbon Monoxide Exposure Estimated Using Regression

    OpenAIRE

    Rudra, Carole B.; Williams, Michelle A.; Sheppard, Lianne; Koenig, Jane Q.; Schiff, Melissa A.; Frederick, Ihunnaya O; Dills, Russell

    2010-01-01

    Exposure to carbon monoxide (CO) and other ambient air pollutants is associated with adverse pregnancy outcomes. While there are several methods of estimating CO exposure, few have been evaluated against exposure biomarkers. The authors examined the relation between estimated CO exposure and blood carboxyhemoglobin concentration in 708 pregnant western Washington State women (1996–2004). Carboxyhemoglobin was measured in whole blood drawn around 13 weeks’ gestation. CO exposure during the mon...

  14. Recombinant Human Erythropoietin as an Alternative to Blood Transfusion in Cancer-Related Anaemia

    OpenAIRE

    Andreas Engert

    2000-01-01

    As physicians become more aware of the need to treat anaemia in their patients with cancer, they have to consider 2 treatment options: red blood cell transfusion or recombinant human erythropoietin [epoetin alfa; epoetin beta]. Healthcare systems are under increasing pressure to lower costs while maintaining quality of care; therefore, treatment of cancer-related anaemia requires a disease management strategy aimed at achieving optimal clinical and economic outcomes. Although blood transfusio...

  15. Development of standard definitions for surveillance of complications related to blood donation.

    Science.gov (United States)

    Goldman, M; Land, K; Robillard, P; Wiersum-Osselton, J

    2016-02-01

    Standard definitions of donor reactions allow each blood establishment to monitor donor adverse events and compare with other organizations to develop best practices. The ISBT Haemovigilance Working Party leads a multi-organizational effort to update the 2008 ISBT standard for surveillance of complications related to blood donation. Revised definitions have been developed and endorsed by the ISBT, AABB, International Haemovigilance Network (IHN) and other international organizations. PMID:26361365

  16. Cerebral blood flow during delirium tremens and related clinical states studied with xenon-133 inhalation tomography

    International Nuclear Information System (INIS)

    The regional cerebral blood flow of 12 patients with severe alcohol withdrawal reactions (delirium tremens or impending delirium tremens) was measured during the acute state before treatment and after recovery. Greater cerebral blood flow was significantly correlated with visual hallucinations and agitation during the acute withdrawal reaction. The results suggest that delirium tremens and related clinical states represent a type of acute brain syndrome mainly characterized by CNS hyperexcitability

  17. Cerebral blood flow during delirium tremens and related clinical states studied with xenon-133 inhalation tomography

    Energy Technology Data Exchange (ETDEWEB)

    Hemmingsen, R.; Vorstrup, S.; Clemmesen, L.; Holm, S.; Tfelt-Hansen, P.; Sorensen, A.S.; Hansen, C.; Sommer, W.; Bolwig, T.G.

    1988-11-01

    The regional cerebral blood flow of 12 patients with severe alcohol withdrawal reactions (delirium tremens or impending delirium tremens) was measured during the acute state before treatment and after recovery. Greater cerebral blood flow was significantly correlated with visual hallucinations and agitation during the acute withdrawal reaction. The results suggest that delirium tremens and related clinical states represent a type of acute brain syndrome mainly characterized by CNS hyperexcitability.

  18. Blood Pressure Changes in Relation to Arsenic Exposure in a U.S. Pregnancy Cohort

    OpenAIRE

    Farzan, Shohreh F.; Chen, Yu; Wu, Fen; Jiang, Jieying; Liu, Mengling; Baker, Emily; Korrick, Susan A.; Margaret R Karagas

    2015-01-01

    Background: Inorganic arsenic exposure has been related to the risk of increased blood pressure based largely on cross-sectional studies conducted in highly exposed populations. Pregnancy is a period of particular vulnerability to environmental insults. However, little is known about the cardiovascular impacts of arsenic exposure during pregnancy. Objectives: We evaluated the association between prenatal arsenic exposure and maternal blood pressure over the course of pregnancy in a U.S. popul...

  19. Non Infectious Complications Related to Blood Transfusion: An 11 year Retrospective Analysis in a Tertiary Care Hospital.

    Science.gov (United States)

    Philip, J; Pawar, A; Chatterjee, T; Mallhi, R S; Biswas, A K; Dimri, U

    2016-09-01

    In India transmission of transfusion transmissible infections (TTI) has shown a relative decrease as a result of mandatory screening of donated blood for TTI's. However, reducing the incidence of non infectious complications poses a major challenge, mainly due to the fact that a number of adverse reactions go unreported. Blood transfusion reaction, can be categorized based on the time interval between transfusion of blood products and the presentation of adverse reactions as acute i.e. those presenting during or within 24 h and as delayed i.e. those presenting anytime after 24 h. Transfusion reactions can further be classified as immune and non immune or infectious and non infectious based on the pathophysiology. In this retrospective study which was undertaken with an aim to determine the type and frequency of non infectious complications due to transfusion of blood and blood products recorded the incidence of febrile non hemolytic transfusion reactions (FNHTR) 51.40 %, allergic reactions 40.14 %, non immune hemolytic reactions 4.22 %, hypothermia 2.81 %, anaphylaxis 0.70 % and iron overload 0.70 %. FNHTR which was found to be the most common complication in this study can certainly be minimized, if not completely eliminated by adopting a policy of universal leucodepletion, the implementation of which solely depends on the financial and infrastructure resources available. This study also reiterates the importance of hemovigilance as a tool to improve the safety of blood transfusion. PMID:27429521

  20. [Use of leukocyte-filtered, cytomegalovirus-antibody negative and irradiated cellular blood products].

    Science.gov (United States)

    Solheim, B G; Albrechtsen, D H; Evensen, S A; Leivestad, T

    1990-01-10

    This paper presents both quality requirements and indications for use of leucocyte-filtered, cytomegalovirus antibody negative and irradiated cellular blood products at Rikshospitalet. Emphasis is placed on the use of standardized buffycoat depleted red cells or platelet concentrates for filtration, and the selection of leucocyte filters with high capacity and ease of bedside application. Leucocyte counts as low as 1-2 10(5) per unit are recommended after filtration in order to avoid HLA-antibody production. For bedside filtration, our choice was RC100 and PL100 (Pall) for red cells and platelets respectively. For laboratory use we prefer, for economic reasons, to use Sepacell R500 (Asahi) and Imugard IG500 (Terumo) for red cells and platelets respectively. Leucocyte-filtered blood products are considered indicated in all pre-transplant transfusions, in post-transplant HLA-sensitized patients, in other patients with febrile transfusion reactions, and in patients with an expected protracted platelet requirement. CMV antibody negative products are recommended for all immuno-deficient patients and pregnant women negative for CMV antibody. Irradiated blood products are used in the treatment of immuno-deficient patients receiving large amounts of blood, and in all severely immuno-compromised patients. In emergency situations where CMV antibody negative and/or irradiated blood products cannot be supplied, leucocyte filtration is suggested. PMID:2154061

  1. A study on dietary habits, health related lifestyle, blood cadmium and lead levels of college students.

    Science.gov (United States)

    Shin, Nari; Hyun, Whajin; Lee, Hongmie; Ro, Mansoo; Song, Kyunghee

    2012-08-01

    This study was performed in order to investigate dietary habits, health related lifestyle and blood cadmium and lead levels in female college students. 80 college students (43 males and 37 females) participated in the survey questionnaires. Body weight and height, blood pressure, and body composition were measured. The systolic blood pressure of male and female students were 128.9 ± 13.9 and 109.8 ± 12.0, respectively. The diastolic blood pressure of male and female students were 77.1 ± 10.3 and 66.0 ± 6.9, respectively, showing that male students had significantly higher blood pressure than female students (P cadmium level of male and female students were 0.54 ± 0.23 and 0.52 ± 0.36, respectively. There was no significant difference between male and female students. The blood lead level of male and female students were 1.09 ± 0.49 and 0.59 ± 0.45, respectively. The blood lead level of male students was significantly higher than that of female students (P cadmium level of smokers and nonsmokers were 0.69 ± 0.29 and 0.49 ± 0.29 respectively (P cadmium level of smokers was significantly higher than that of nonsmokers (P < 0.05). The blood lead level of smokers and nonsmokers were 1.09 ± 0.43 and 0.80 ± 0.54, respectively. The blood lead level of smokers was significantly higher than that of nonsmokers (P < 0.05). Therefore, proper nutritional education programs are required for college students in order to improve their dietary and health related living habits. PMID:22977689

  2. Cord blood cytokines are modulated by maternal farming activities and consumption of farm dairy products during pregnancy: the PASTURE Study.

    OpenAIRE

    Pfefferle, Petra Ina; Büchele, Gisela; Blümer, Nicole; Roponen, Marjut; Ege, Markus Johannes; Krauss-Etschmann, Susanne; Genuneit, Jon; Hyvärinen, Anne; Hirvonen, Maija-Riitta; Lauener, Roger; Pekkanen, Juha; Riedler, Josef; Dalphin, Jean Charles; Brunekeef, Bert; Braun-Fahrländer, Charlotte

    2010-01-01

    BACKGROUND: Traditional farming represents a unique model situation to investigate the relationship of early-life farm-related exposure and allergy protection. OBJECTIVES: To investigate associations between maternal farm exposures and cytokine production in cord blood (CB) mononuclear cells in a prospective multinational birth cohort of 299 farm and 326 nonfarm children and their families. METHODS: Supernatants from phorbol 12-myristate 13-acetate/ionomycin-stimulated CB mononuclear cells we...

  3. RELATIONS BETWEEN SELECTED INDICATORS OF BLOOD AND MILK OF DAIRY COWS WITH METABOLIC DISORDERS

    Directory of Open Access Journals (Sweden)

    Jaroslav Kováčik

    2013-02-01

    Full Text Available The aim of this work was to monitor the relations between selected indicators of technological properties of milk and blood biochemical parameters of dairy cows with metabolic disorders. Thirty-two cows were chosen, which were divided into 3 groups: first group - cows with metabolic problems of acidosis, second group - cows with metabolic problems of alkalosis, third group - healthy cows. Blood, urine and milk samples were collected. Urea, total lipids, total proteins, glucose and calcium was determined in the blood serum. Pure acidobasic forms, pH and density of urine were determined. Proteins, lactose, non-fat-solids, somatic cells count, calcium, urea, titratable acidity, fermentability, rennetability and thermostability were determined in samples of milk. Significant negative dependences were observed in the group of cows with metabolic problems of acidosis between urea in blood and in milk (r = -0.694, P <0.05, between calcium in blood and in milk (r = -0.653, P <0, 05, and between calcium in milk and glucose in blood (r = -0.648, P <0.05. In the group of cows with alkalosis, statistically significant correlation between total lipids in blood and fat in milk was found (r = -0.879, P <0.05.

  4. EFSA NDA Panel (EFSA Panel on Dietetic Products, Nutrition and Allergies), 2013. Scientific Opinion on the substantiation of a health claim related to a combination of red spinach, green spinach, red chicory, green chicory, green leaf chard, red leaf chard, red Swiss chard, golden Swiss chard and white Swiss chard and protection of blood lipids from oxidative damage pursuant to Article 13(5) of Regulation (EC) No 1924/2006

    OpenAIRE

    Tetens, Inge

    2013-01-01

    Following an application from Italsur s.r.l., submitted pursuant to Article 13(5) of Regulation (EC) No 1924/2006 via the Competent Authority of Italy, the Panel on Dietetic Products, Nutrition and Allergies (NDA) was asked to deliver an opinion on the scientific substantiation of a health claim related to a combination of red spinach, green spinach, red chicory, green chicory, green leaf chard, red leaf chard, red Swiss chard, golden Swiss chard and white Swiss chard and protection of blood ...

  5. EFSA Panel on Dietetic Products, Nutrition and Allergies (NDA); Scientific Opinion on the substantiation of a health claim related to monacolin K in SYLVAN BIO red yeast rice and maintenance of normal blood LDL - cholesterol concentrations pursuant to Article 13(5) of Regulation (EC) No 1924/2006

    DEFF Research Database (Denmark)

    Tetens, Inge

    on the scientific substantiation of a health claim related to monacolin K in SYLVAN BIO red yeast rice and maintenance of normal blood LDL-cholesterol concentrations. The food, monacolin K in SYLVAN BIO red yeast rice, that is the subject of the health claim is sufficiently characterised. The claimed...... effect, maintenance of normal blood LDL-cholesterol concentrations, is a beneficial physiological effect. A claim on monacolin K from red yeast rice and maintenance of normal blood LDL-cholesterol concentrations has already been assessed with a favourable outcome at daily intakes of 10 mg monacolin K...... from any red yeast rice preparation (which would include SYLVAN BIO red yeast rice). The evidence provided by the applicant for the present application does not establish that monacolin K in SYLVAN BIO red yeast rice is different from monacolin K in other red yeast rice preparations with respect to its...

  6. EFSA Panel on Dietetic Products, Nutrition and Allergies (NDA); Scientific Opinion on the substantiation of health claims related to sodium bicarbonate and maintenance of normal blood pressure (ID 1404) pursuant to Article 13(1) of Regulation (EC) No 1924/2006

    DEFF Research Database (Denmark)

    Tetens, Inge

    claims in relation to sodium bicarbonate and maintenance of normal blood pressure. The scientific substantiation is based on the information provided by the Member States in the consolidated list of Article 13 health claims and references that EFSA has received from Member States or directly from...... stakeholders. The food constituent that is the subject of the health claim is sodium bicarbonate. The Panel considers that sodium bicarbonate is sufficiently characterised. The claimed effect is “blood pressure reduction”. The target population is assumed to be the general population. In the context of the...... substantiation of the claim. On the basis of the data presented, the Panel concludes that a cause and effect relationship has not been established between the consumption of sodium bicarbonate and maintenance of normal blood pressure....

  7. Blood Levels of Zinc in Creole Horses Used in Sera Production

    International Nuclear Information System (INIS)

    Using Neutron Activation Analysis Zn concentrations were determined in blood of horses (Creole breed). No significant difference was observed between male (0.0029±.0007 gL-1) and female (0.0031±.0011 gL-1) animals. These data are an important support to understand the physiological functions of Zn in blood during the process of sera production at Butantan Institute (Sao Paulo, Brazil) using horses.

  8. Continuous blood pressure monitoring in cirrhosis. Relations to splanchnic and systemic haemodynamics

    DEFF Research Database (Denmark)

    Møller, S; Christensen, E; Henriksen, Jens Henrik Sahl

    1997-01-01

    a high post-sinusoidal resistance, a low plasma volume, a short central circulation time, and the presence of ascites. In contrast, a low intra-arterial blood pressure was determined by a low serum sodium, a low haemoglobin, and a high cardiac output. Diuretic treatment did not influence this model......BACKGROUND/AIMS: Low arterial blood pressure is recognised as a distinctive factor in the hyperdynamic circulation in cirrhosis. 24-hour monitoring of the blood pressure and heart rate has recently revealed a reduced circadian variation with relation to liver function. However, associations with...... other clinical and haemodynamic characteristics have not been investigated and the aim of the present study was to identify splanchnic and systemic determinants of the 24-h blood pressure and heart rate in cirrhosis. METHODS: The variables were measured by an automatic ambulant device for monitoring...

  9. Parvovirus 4–like Virus in Blood Products

    OpenAIRE

    Szelei, Jozsef; Liu, Kaiyu; Li, Yi; Fernandes, Sandra; Tijssen, Peter

    2010-01-01

    Porcine plasma and factor VIII preparations were screened for parvovirus 4 (PARV)–like viruses. Although the prevalence of PARV4-like viruses in plasma samples was relatively low, viruses appeared to be concentrated during manufacture of factor VIII. PARV4-like viruses from human and porcine origins coevolved likewise with their hosts.

  10. Relations between radiobiological hypoxia and nuclear magnetic resonance-imaged blood microcirculation in experimental tumors

    International Nuclear Information System (INIS)

    Characteristics of hypoxic cells subjected to radiation were investigated and compared with those of microcirculation for two murine fibrosarcomas growing in C3H mice. Small NFSa tumors, growing in air-breathing mice, developed a radioresistant tail on the survival curve. The tail was indistinguishably parallel to a survival curve for an artificially hypoxic tumor. As the NFSa tumors increased in size, the hypoxic tail moved upward with no change of Do, resulting in increase of hypoxic fraction from 3.9% to 40%. The R1137 tumors had no radioresistant tail nor hypoxic fraction regardless of tumor size. However, large-sized R1137 tumors developed a significant number of radioresistant, hypoxic cells with an intermediate Do, and were effectively sensitized by administrating misonidazole before irradiation. Thus, the NFSa tumors were fractionally hypoxic, and the large R1137 tumors had intermediate hypoxia. Measurement of tumor microcirculation by gadolinium-enhanced nuclear magnetic resonance indicated that both blood flow and blood volume decreased significantly when the NFSa tumor grew large. Similar reduction in these microcirculation parameters was also observed for the R1137 tumor. The small-sized NFSa tumor had relatively larger blood volume and faster blood flow than the small-sized R1137 tumor. When large-sized tumors were compared to each other, the NFSa again had better blood flow than the R1137. However, the blood volume in the large-sized tumors was significantly (p<0.05) smaller for the NFSa tumor than for the R1137 tumor. It was concluded that blood flow could not be a single determinant for tumor hypoxia, and the difference between fractional hypoxia and intermediate hypoxia would be reflected in the ratio of blood flow to blood volume. (author)

  11. Blood serum and BSA, but neither red blood cells nor hemoglobin can support vitellogenesis and egg production in the dengue vector Aedes aegypti

    Directory of Open Access Journals (Sweden)

    Kristina K. Gonzales

    2015-05-01

    Full Text Available Aedes aegypti is the major vector of dengue, yellow fever and chikungunya viruses that put millions of people in endemic countries at risk. Mass rearing of this mosquito is crucial for strategies that use modified insects to reduce vector populations and transmission of pathogens, such as sterile insect technique or population replacement. A major problem for vector mosquito mass rearing is the requirement of vertebrate blood for egg production since it poses significant costs as well as potential health hazards. Also, regulations for human and animal use as blood source can pose a significant obstacle. A completely artificial diet that supports egg production in vector mosquitoes can solve this problem. In this study, we compared different blood fractions, serum and red blood cells, as dietary protein sources for mosquito egg production. We also tested artificial diets made from commercially available blood proteins (bovine serum albumin (BSA and hemoglobin. We found that Ae. aegypti performed vitellogenesis and produced eggs when given whole bovine blood, serum, or an artificial diet containing BSA. Conversely, egg production was impaired after feeding of the red blood cell fraction or an artificial diet containing only hemoglobin. We also found that egg viability of serum-fed mosquitoes were comparable to that of whole blood and an iron supplemented BSA meal produced more viable eggs than a meal containing BSA alone. Our results indicate that serum proteins, not hemoglobin, may replace vertebrate blood in artificial diets for mass mosquito rearing.

  12. Blood serum and BSA, but neither red blood cells nor hemoglobin can support vitellogenesis and egg production in the dengue vector Aedes aegypti.

    Science.gov (United States)

    Gonzales, Kristina K; Tsujimoto, Hitoshi; Hansen, Immo A

    2015-01-01

    Aedes aegypti is the major vector of dengue, yellow fever and chikungunya viruses that put millions of people in endemic countries at risk. Mass rearing of this mosquito is crucial for strategies that use modified insects to reduce vector populations and transmission of pathogens, such as sterile insect technique or population replacement. A major problem for vector mosquito mass rearing is the requirement of vertebrate blood for egg production since it poses significant costs as well as potential health hazards. Also, regulations for human and animal use as blood source can pose a significant obstacle. A completely artificial diet that supports egg production in vector mosquitoes can solve this problem. In this study, we compared different blood fractions, serum and red blood cells, as dietary protein sources for mosquito egg production. We also tested artificial diets made from commercially available blood proteins (bovine serum albumin (BSA) and hemoglobin). We found that Ae. aegypti performed vitellogenesis and produced eggs when given whole bovine blood, serum, or an artificial diet containing BSA. Conversely, egg production was impaired after feeding of the red blood cell fraction or an artificial diet containing only hemoglobin. We also found that egg viability of serum-fed mosquitoes were comparable to that of whole blood and an iron supplemented BSA meal produced more viable eggs than a meal containing BSA alone. Our results indicate that serum proteins, not hemoglobin, may replace vertebrate blood in artificial diets for mass mosquito rearing. PMID:26020000

  13. Product Stigmaticity: Understanding, Measuring and Managing Product-Related Stigma

    NARCIS (Netherlands)

    Vaes, K.

    2014-01-01

    Stigma-free Product Design. Many of the products intended to relieve us from discomforting or unsafe situations and many medical and assistive devices are experienced as unpleasant and uncomfortable. On top of their discomfort, product users may also experience social unease from the people around t

  14. Pulmonary blood volume and transit time in cirrhosis: relation to lung function

    DEFF Research Database (Denmark)

    Møller, Søren; Burchardt, H; Øgard, CG;

    2006-01-01

    BACKGROUND/AIMS: In cirrhosis a systemic vasodilatation leads to an abnormal distribution of the blood volume with a contracted central blood volume. In addition, the patients have a ventilation/perfusion imbalance with a low diffusing capacity. As the size of the pulmonary blood volume (PBV) has...... not been determined separately we assessed PBV and pulmonary transit time (PTT) in relation to lung function in patients with cirrhosis and in controls. METHODS: Pulmonary and cardiac haemodynamics and transit times were determined by radionuclide techniques in 22 patients with alcoholic cirrhosis and...... cirrhosis. The relation between PBV and PTT and the low diffusing capacity suggests the pulmonary vascular compartment as an important element in the pathophysiology of the lung dysfunction in cirrhosis....

  15. Polycyclic aromatic hydrocarbons in blood related to lower body mass in common loons.

    Science.gov (United States)

    Paruk, James D; Adams, Evan M; Uher-Koch, Hannah; Kovach, Kristin A; Long, Darwin; Perkins, Christopher; Schoch, Nina; Evers, David C

    2016-09-15

    We captured 93 wintering adult and subadult Common Loons (Gavia immer) in coastal Louisiana from 2011 to 2015 following the Deepwater Horizon oil spill. We tested blood samples for exposure to polycyclic aromatic hydrocarbons (PAHs) and measured physiological variables including hematocrit, hemoglobin and total blood protein. PAH concentrations in loon blood differed from year to year and by age class. High PAH concentrations were significantly related to lower body masses in both adult and subadult birds and higher serum protein levels in adults only. PAH concentrations had marginal relations with both hematocrit and hemoglobin levels. The types of PAHs detected also underwent a major shift over time. The PAHs detected in 2011, 2012, and 2015 were primarily low molecular weight (three carbon rings); however, in 2013, most detected PAHs were high molecular weight (four carbon rings). It is unclear what events led to the increase in PAH concentrations and the shift in type of PAHs over time. PMID:27177142

  16. Pulmonary blood volume and transit time in cirrhosis: relation to lung function

    DEFF Research Database (Denmark)

    Møller, Søren; Burchardt, H; Øgard, CG;

    2006-01-01

    BACKGROUND/AIMS: In cirrhosis a systemic vasodilatation leads to an abnormal distribution of the blood volume with a contracted central blood volume. In addition, the patients have a ventilation/perfusion imbalance with a low diffusing capacity. As the size of the pulmonary blood volume (PBV) has...... cirrhosis. The relation between PBV and PTT and the low diffusing capacity suggests the pulmonary vascular compartment as an important element in the pathophysiology of the lung dysfunction in cirrhosis....... not been determined separately we assessed PBV and pulmonary transit time (PTT) in relation to lung function in patients with cirrhosis and in controls. METHODS: Pulmonary and cardiac haemodynamics and transit times were determined by radionuclide techniques in 22 patients with alcoholic cirrhosis and...

  17. Association between Blood Lead Levels and Age-Related Macular Degeneration.

    Directory of Open Access Journals (Sweden)

    Ho Sik Hwang

    Full Text Available To investigate the association between blood lead levels and prevalence of age-related macular degeneration (AMD.A nationwide population-based cross-sectional study included 4,933 subjects aged over 40 years who participated in the 2008-2012 Korean National Health and Nutrition Examination Survey, and for whom fundus photographs were available. All participants underwent a standardized interview, evaluation of blood lead concentration, and a comprehensive ophthalmic examination. Digital fundus photographs (45° were taken of both eyes under physiological mydriasis. All fundus photographs were graded using an international classification and grading system.Mean blood lead levels were 3.15 μg/dL in men and 2.27 μg/dL in women (P < 0.001. After adjusting for potential confounders including age, gender, smoking status, total cholesterol levels, triglyceride levels, heart problems and strokes, the adjusted odds ratio (OR in women for any AMD was 1.86 (95% Confidence Interval [CI], 1.03-3.36 and for early AMD was 1.92 (95% CI, 1.06-3.48, for those in the highest quintile of lead level compared with the lowest quintile. In men, however, blood lead level was not significantly associated with AMD.Blood lead levels were higher in men, but were only associated with AMD in women. Increased levels of blood lead may be involved in the pathogenesis of AMD development in women.

  18. Optimization of sugar and blood feeding regimen in Anopheles gambiae mass production system

    International Nuclear Information System (INIS)

    The sterile insect technique (SIT) is being developed for the control of malaria transmitting mosquitoes. Critical to the success of applying the SIT is the establishment of standardized mass production systems for the target species. As part of efforts to develop standardised mass production systems for malaria vectors, this project sought to optimize adult blood and sugar feeding in a mass production system. Different sugar types (glucose, sucrose and honey) were evaluated at 6% and 10% concentrations in water to determine the best sugar diet and concentration for feeding adult An. gambiae. Different blood feeding methods, restrained Guinea pig, anaesthetised Guinea pig and human arm feeding were evaluated. Adult survival, female insemination and egg production were used as criteria to determine optimum sugar and blood feeding. The effect of anaesthetics on blood feeding response and egg production of female An. gambiae was determined by comparing feeding response and egg production of females fed with anaesthetised Guinea pigs as against physically restrained Guinea pigs (Control). The specific effect of different anaesthetic agents on blood feeding response and egg production of female mosquitoes were was also determined by comparing the feeding response and egg production of females fed with either Ketamine/Xylazine anaesthetised Guinea pigs or Ketamine/Diazepam anaesthetised Guinea pigs. Effects due to sugar types and concentrations on percentage survival of male and female mosquitoes were observed to be significant at (p 0.05). However, human ann feeding (HAP) method and Ketamine/Xylazine (KX) anaesthetics fed for 25 minutes recorded higher percentage feeding (76.0% and 68.0% respectively) and egg production of 19.0% and 20.8% respectively. Anaesthetised Guinea pig feeding (AGF) of adults for 15 minutes followed closely with 60.0% and 15.1% blood feeding and egg production respectively whilst restrained Guinea pig feeding (RGF) method and Ketamine

  19. Homocysteine induces production of monocyte chemoattractant protein-1 and interleukin-8 in cultured human whole blood

    Institute of Scientific and Technical Information of China (English)

    Xiao-kunZENG; DanielGREMICK; XianWANG

    2004-01-01

    AIM: To investigate whether increased plasma L-homocysteine (Hcy) level could promote monocyte chemoattract antprotein-1 (MCP-1) and interleukin-8 (IL-8) in cultured whole blood. METHODS: Human whole blood or differenttype of peripheral blood cells from health volunteers were incubated with Hcy and/or the inhibitors. MCP-1 and IL-8 level were measured by ELISA assay. RESULTS: Hcy 10-1000 μmol/L induced production of MCP-1and IL-8 in cultured human whole blood (P<0.05). The major cellular source of these chemokines comed from monocytes. Meanwhile,Hcy also promoted the upregulation of MPO level even at the 10 μmol/L in the cultured whole blood.The intracellular ROS, particular the OH radicals, play extremely important role in the Hcy-induced MCP-1 and IL-8 production. CONCLUSION: Increased Hcy level in plasma (hyperhomocysteinemia) induced MCP-1 and IL-8secretion in cultured human whole blood, especially in monocytes via oxidative stress mechanism,

  20. X-ray irradiation on blood products for the purpose of prevention of PT-GVHD

    International Nuclear Information System (INIS)

    X-ray irradiation on blood products is a common method to prevent the risk of inducing PT-GVHD, and has recently become to be used in Japan. We have tried X-ray irradiation on WB, CRC and PC using HITACHI X-ray Irradiation Apparatus MBR1520R, and studied its practical usefulness. In case of irradiation on blood bags as the minimum dose of 1,500 rads, these conditions are thought to be practical: 1) the use of 1.0 mm Al filter, 2) the distance of 550 nm from X-ray source, and 3) irradiation on 4 bags at the same time. But, it has also been noticed that total doses and qualities of X-ray absorbed into blood were different between upper and lower side of the bag. Bloods on upper side absorbed much doses and a wide range of X-ray, on the other hand, bloods on lower side absorbed less doses and hard X-ray. In these conditions, irradiated lymphocytes showed a complete inhibition of thymidine uptake in MLC test, still had 15% of activity in PHA stimulation. The qualities of other blood components have not changed before and after irradiation. X-ray irradiation is useful in a routine work of blood center, but problems of proper doses and a uniformity of irradiation are remained to be solved. (author)

  1. EFSA Panel on Dietetic Products, Nutrition and Allergies (NDA); Scientific Opinion on the substantiation of a health claim related to barley beta-glucan and lowering of blood cholesterol and reduced risk of (coronary) heart disease pursuant to Article 14 of Regulation (EC) No 1924/2006

    DEFF Research Database (Denmark)

    Tetens, Inge

    Following an application from Cargill Incorporated submitted pursuant to Article 14 of Regulation (EC) No 1924/2006 via the Competent Authority of Belgium, the Panel on Dietetic Products, Nutrition and Allergies was asked to deliver an opinion on the scientific substantiation of a health claim...... intervention studies, two animal studies and one mechanistic study. In weighing the evidence, the Panel took into account that one meta-analysis including 11 RCTs and one additional RCT which investigated the effects of barley beta-glucans at doses of at least 3 g/day showed a decrease in total and LDL...... consumption of barley beta-glucans and the lowering of blood LDL-cholesterol concentrations. The following wording reflects the scientific evidence: “Barley beta-glucans have been shown to lower/reduce blood cholesterol. High cholesterol is a risk factor in the development of coronary heart disease”. At least...

  2. EFSA Panel on Dietetic Products, Nutrition and Allergies (NDA); Scientific Opinion on the substantiation of health claims related to chitosan and reduction in body weight (ID 679, 1499), maintenance of normal blood LDL-cholesterol concentrations (ID 4663), reduction of intestinal transit time (ID

    DEFF Research Database (Denmark)

    Tetens, Inge

    claims in relation to chitosan and reduction in body weight, maintenance of normal blood LDL-cholesterol concentrations, reduction of intestinal transit time and reduction of inflammation. The scientific substantiation is based on the information provided by the Member States in the consolidated list of...... Article 13 health claims and references that EFSA has received from Member States or directly from stakeholders. The food constituent that is the subject of the health claim is chitosan. The Panel considers that chitosan is sufficiently characterised....

  3. Obesity, Blood Pressure and Health-Related Behaviour among German Children and Adolescents

    Science.gov (United States)

    Graf, Christine; Jouck, Stefanie; Koch, Benjamin; Platschek, Anna-Maria; Arnold, Christiane; Bohm, Michael; Dordel, Sigrid; Tokarski, Walter

    2008-01-01

    Study aim: To examine the prevalence of obesity and its correlation with blood pressure, waist circumference and other health related risk factors (smoking, alcohol consumption, physical inactivity and TV/PC-screen time) in German youths. Material and methods: A cohort of 831 boys and 808 girls, fifth- to tenth-graders from 3 German high schools…

  4. Storage time effect on blood diet for tsetse mass production in sterile insect technique

    Directory of Open Access Journals (Sweden)

    M. Byamungu, S. Matembo, K. Benedict & G. Mashenga

    2011-06-01

    Full Text Available Background & objectives: Different methods have been developed for tsetse and trypanosomiasis control oreradication but all these have their specific advantages and limitations. However, a combination of methodscould be used as part of an Area-wide Integrated Pest Management (AWPM. The sterile insect technique(SIT as a technique of choice for eradication of tsetse flies requires mass production of flies in the laboratories.Since tsetses are exclusively haemotophogous insects, a quality blood diet is needed for maintenance of flieswith optimum production. The aim of this study was to establish the optimum storage time of blood diet fortsetse colonies.Methods: A total of 450 flies Glossina austeni were fed on different batches of blood (collected in 2004, 2005,2006, 2007 and 2008 three times a week. Pupae were collected daily and mortality checks were done onweekly basis. The quality of the blood diet was measured by observation of tsetse production parametersincluding survival, pupae production and size.Results: The survival of flies fed on 2004 and 2006 batches had lower survival compared to the rest of thebatches. Also the 2005 batch had a significant higher number of pupae compared to the production in othertreatments. The 2004 and 2005 batches had more of small pupae (class A & B compared to the other batches.Interpretation & conclusion: There was a significant difference between the blood batches and the productionparameters were better in the last three years, i.e. 2008, 2007 and 2006. Therefore, this study recommendsthree years to be an optimum storage time for blood diet under regional conditions. Also the storage temperatureconditions should remain stable at –20ºC.

  5. Plasma fractionation for blood products: isolation and purification of coagulating factors, albumin and immunoglobulin

    International Nuclear Information System (INIS)

    Approximately 12 million liters of human plasma are fractionated world-wide annually. However, with the market for clotting factors and other haemoderivatives steadily increasing from year to year, the amount processed will also increase correspondingly to keep up with the demand. In Malaysia, part of the need for the blood products are obtained commercially but a major portion of the requirement involves sending the plasma collected by the National Blood Centre to Australia for processing. Following purification and isolation of the blood products, they are sent back to Malaysia for local consumption. As yet there are no plasma fractionation plants in the South East Asia region, it would be advantageous to establish a local fractionation plant as it would be able to cater for local demands of the haemoderivatives and thus reduces the cost of importing these products. Besides, this facility will be able to provide contract fractionation services to the surrounding region. Early work in MINT has started in trying to purify plasma obtained from rats. Purification of the plasma was performed by using Sephadex G-25 column. Short term objective of this project is to develop the technique of extraction, fractionation and purification of blood products such as albumin, globulin and clotting factors (Factor VIII and Factor IX). The long term emphasis will be to scale up the production facility to a pilot plant stage and eventually to a national fractionation and purification plant. (Author)

  6. The miRNA Profile of Platelets Stored in a Blood Bank and Its Relation to Cellular Damage from Storage.

    Science.gov (United States)

    Pontes, Thaís Brilhante; Moreira-Nunes, Caroline de Fátima Aquino; Maués, Jersey Heitor da Silva; Lamarão, Letícia Martins; de Lemos, José Alexandre Rodrigues; Montenegro, Raquel Carvalho; Burbano, Rommel Mário Rodriguez

    2015-01-01

    Millions of blood products are transfused each year, and many lives are directly affected by transfusion. Platelet concentrate (PC) is one of the main products derived from blood. Even under good storage conditions, PC is likely to suffer cell damage. The shape of platelets changes after 5 to 7 days of storage at 22°C. Taking into consideration that some platelet proteins undergo changes in their shape and functionality during PC storage. Sixteen PC bags were collected and each PC bag tube was cut into six equal pieces to perform experiments with platelets from six different days of storage. Thus, on the first day of storage, 1/6 of the tube was used for miRNA extraction, and the remaining 5/6 was stored under the same conditions until extraction of miRNAs on each the following five days. Samples were sequenced on an Illumina Platform to demonstrate the most highly expressed miRNAs. Three miRNAs, mir127, mir191 and mir320a were validated by real-time quantitative PCR (RQ-PCR) in 100 PC bags tubes. Our method suggests, the use of the miRNAs mir127 and mir320a as biomarkers to assess the "validity period" of PC bags stored in blood banks for long periods. Thus, bags can be tested on the 5th day of storage for the relative expression levels of mir127 and mir320a. Thus, we highlight candidate miRNAs as biomarkers of storage damage that can be used as tools to evaluate the quality of stored PC. The use of miRNAs as biomarkers of damage is unprecedented and will contribute to improved quality of blood products for transfusions. PMID:26121269

  7. [Single-donor (apheresis) platelets and pooled whole-blood-derived platelets--significance and assessment of both blood products].

    Science.gov (United States)

    Hitzler, Walter E

    2014-01-01

    high population density and thereby reduce the theoretical advantage of ATK (but definitely would not nullify it!). It is equally plausible, however, that this agent would behave like a prion, non-sexual transmission, or like a West-Nil virus, a non-contagious vector-transmitted agent. For PTK this would mean a relative risk up to 4 times (PTK from 4 BCs) or 5 times (PTK from 5 BCs) higher than the risk estimated by the Robert-Koch-Institute. If, taking the passive surveillance data and the changing variables (donor frequency, donor population, and donor location) into account, the risk of transmission of an infection via ATK (exposure to 1 donor) with HIV, HCV, and HBV moves closer to the higher risk of PTK (exposure to 4 or 8 donors, in case of double ATK per patient), this result of the risk model calculation by no means indicates any equivalency between PTK and ATK with respect to the risk of transmission of infection. The modifiable variables of donor frequency, donor population, and donor location need to be modified, as scientific deductions, in such a way that the avoidable risk of ATK which is influenced by these variables can be corrected to the minimum risk of a transmission of infection of HIV, HBV, and HCV via ATK in comparison to PTK. The minimum risk of a possible transmission of infection via ATK (exposure to 1 donor) is the basic intrinsic risk of each individual blood donation. The basic intrinsic risk increases relative to the number of blood donations or exposure to donors (PtK has an unalterable, production-dependent exposure to 4 or 8 donors). Let us consider a 1:1.000 prevalence for a new pathogen, which is spread equally in each donor population (apheresis and whole blood) and the present case of approximately 500,000 transfused platelet concentrates in Germany. This means that for the production of 4 PTK about 2 million donations are processed, 2,000 infectious Buffy-Coats are obtained and, thereby, 2,000 infectious PTK. In the case of ATK

  8. [Effects of various food products on blood sugar level in patients with diabetes mellitus and obesity].

    Science.gov (United States)

    Kurbanov, S K; Khasaev, A Sh; Gapparov, M M

    1991-01-01

    The blood sugar level was evaluated in patients with insulin-independent diabetes mellitus and obesity, and in normal volunteers, during 3 h with a 30-min interval before and after food loading containing 50 g of carbohydrates. It has been found that 17 food products studied produce varying effects on the blood glucose level regardless of the content of starch and sugars in them. Pure glucose, boiled rice, potatoe, white bread, boiled bean, pastry, honey and sugar produce the highest glycemic effect. Milk, ice cream, boiled lens and noodles produce a moderate effect on the blood sugar level both in normal subjects and in patients with diabetes mellitus and obesity. The increase of the glucose blood level after intake of food products was significantly higher in patients with diabetes mellitus as compared to those with obesity and normal volunteers. Milk products, lens, macaroni and millet are most promising for patients with diabetes mellitus due to their moderate influence on the blood glucose content. PMID:2042318

  9. Relation of fingerprints and shape of the palm to fetal growth and adult blood pressure.

    OpenAIRE

    Godfrey, K M; Barker, D J; Peace, J; Cloke, J.; Osmond, C

    1993-01-01

    OBJECTIVE--To examine how finger and palm prints are related to fetal growth and adult blood pressure. DESIGN--Follow up study of babies born around 50 years ago whose birth weight, placental weight, head circumference, and length at birth were recorded. SETTING--Preston, Lancashire. SUBJECTS--139 men and women born in Sharoe Green Hospital in Preston during 1935-43 and still living in Lancashire. MAIN OUTCOME MEASURES--Finger and palm prints and current blood pressure. RESULTS--People who we...

  10. EFSA Panel on Dietetic Products, Nutrition and Allergies (NDA); Scientific Opinion on the substantiation of health claims related to rye fibre and changes in bowel function (ID 825), reduction of post-prandial glycaemic responses (ID 826) and maintenance of normal blood LDL

    DEFF Research Database (Denmark)

    Tetens, Inge

    claims in relation to rye fibre and changes in bowel function, reduction of post-prandial glycaemic responses and maintenance of normal blood LDL-cholesterol concentrations. The scientific substantiation is based on the information provided by the Member States in the consolidated list of Article 13...... health claims and references that EFSA has received from Member States or directly from stakeholders. The food constituent that is the subject of the health claims is rye fibre. The Panel considers that rye fibre is sufficiently characterised in relation to the claimed effects....

  11. Continuous and semi-continuous cell culture for production of blood clotting factors.

    Science.gov (United States)

    Desai, Sunil G

    2015-11-10

    Recombinant clotting factors are important biotherapeutics that Pfizer has produced and marketed for over fifteen years. Owing to the complexity of the structure and function of these blood factors, it can be challenging to achieve the required product quality and manufacturing productivity. The article highlights the semi-continuous and continuous cell culture processes employed by Pfizer for the production of BeneFIX and ReFacto AF. The benefits of such processes, the challenges of maintaining an aseptic production culture for extended periods, and batch definition are discussed in this article. PMID:25738489

  12. Whole Blood Activation Results in Altered T Cell and Monocyte Cytokine Production Profiles by Flow Cytometry

    Science.gov (United States)

    Crucian, Brian E.; Sams, Clarence F.

    2001-01-01

    An excellent monitor of the immune balance of peripheral circulating cells is to determine their cytokine production patterns in response to stimuli. Using flow cytometry, a positive identification of cytokine producing cells in a mixed culture may be achieved. Recently, the ability to assess cytokine production following a whole-blood activation culture has been described. In this study, whole blood activation was compared to traditional PBMC activation and the individual cytokine secretion patterns for both T cells, T cell subsets and monocytes was determined by flow cytometry. RESULTS: For T cell cytokine assessment (IFNg/IL-10 and IL-21/L-4) following PMA +ionomycin activation: (1) a Significantly greater percentages of T cells producing IFNgamma and IL-2 were observed following whole-blood culture and (2) altered T cell cytokine production kinetics were observed by varying whole blood culture times. Four-color analysiS was used to allow assessment of cytokine production by specific T cell subsets. It was found that IFNgamma production was significantly elevated in the CD3+/CD8+ T cell population as compared to the CD3+/CD8- population following five hours of whole blood activation. Conversely, IL-2 and IL-10 production were Significantly elevated in the CD3+/CD8- T cell population as compared to the CD3+/CD8+ population. Monocyte cytokine production was assessed in both culture systems following LPS activation for 24 hours. A three-color flow cytometric was used to assess two cytokines (IL-1a/IL-12 and TNFa/IL-10) in conjunction with CD14. Nearly all monocytes were stimulated to produce IL-1a, IL-12 and TNFa. equally well in both culture systems, however monocyte production of IL-10 was significantly elevated in whole blood culture as compared to PBMC culture. IL-12 producing monocytes appeared to be a distinct subpopulation of the IL-1a producing set, whereas IL-10 and TNFa producing monocytes were largely mutually exclusive. IL-10 and TNFa producing

  13. Biological effects of the electrostatic field: red blood cell-related alterations of oxidative processes in blood

    Science.gov (United States)

    Harutyunyan, Hayk A.; Sahakyan, Gohar V.

    2016-01-01

    The aim of this study was to determine activities of pro-/antioxidant enzymes, reactive oxygen species (ROS) content, and oxidative modification of proteins and lipids in red blood cells (RBCs) and blood plasma of rats exposed to electrostatic field (200 kV/m) during the short (1 h) and the long periods (6 day, 6 h daily). Short-term exposure was characterized by the increase of oxidatively damaged proteins in blood of rats. This was strongly expressed in RBC membranes. After long-term action, RBC content in peripheral blood was higher than in control ( P < 0.01) and the attenuation of prooxidant processes was shown.

  14. Ex-vivo response to blood products and haemostatic agents after paediatric cardiac surgery

    DEFF Research Database (Denmark)

    Hvas, Anne-Mette; Andreasen, Jo B; Christiansen, Kirsten; Ravn, Hanne B

    2013-01-01

    . The aims of the present study were to investigate changes in coagulation profiles after paediatric cardiac surgery and the effect after ex-vivo addition of blood products and haemostatic agents. Coagulation profiles were evaluated by thromboelastometry (ROTEM) in 54 children before and immediately...

  15. Immunomodulatory capacity of fungal proteins on the cytokine production of human peripheral blood mononuclear cells

    NARCIS (Netherlands)

    Jeurink, P.V.; Lull Noguera, C.; Savelkoul, H.F.J.; Wichers, H.J.

    2008-01-01

    Immunomodulation by fungal compounds can be determined by the capacity of the compounds to influence the cytokine production by human peripheral blood mononuclear cells (hPBMC). These activities include mitogenicity, stimulation and activation of immune effector cells. Eight mushroom strains (Agaric

  16. Changes in some blood parameters and production performance of old laying hens due to growth hormone and testosterone injection.

    Science.gov (United States)

    Mohammadi, H; Ansari-Pirsaraei, Z

    2014-06-01

    The experiment was designed to study the changes in some blood parameters and production performance of old laying hens after injection of different doses of growth hormone (GH) and testosterone (Ts). A total of 160 old laying hens (HyLine W-36) at 73 weeks of age were weighed individually and randomly allocated to four treatments with four replicates and 10 birds in each replicate in a completely randomized design. Growth hormone and Ts hormones were injected subcutaneously. Treatment groups were as follows: treatment 1: injection of 100 μl distiled water (control group), treatment 2: injection of 500 μg Ts/kg live-weight + 50 μgGH/kg live-weight, treatment 3: injection of 500 μgTs/kg live-weight + 100 μgGH/kg live-weight and treatment 4: injection of 500 μgTs/kg live-weight + 150 μgGH/kg live-weight. Plasma levels of oestradiol, T4 , LDL, HDL and cholesterol significantly increased in treatment 3 in relation to the control group. All injected hens showed significantly higher levels of glucose in relation to control group. The results showed the positive effects of GH and Ts administration on production performance and blood parameters which are associated with egg production potentiality and in turn may improve reproductivity (egg production) in old laying hens. The positive results of the study may be useful in animal selection and breeding programmes. PMID:23808354

  17. Bos taurus papillomavirus activity in peripheral blood mononuclear cells: demonstrating a productive infection.

    Science.gov (United States)

    Melo, T C; Araldi, R P; Pessoa, N S D; de-Sá-Júnior, P L; Carvalho, R F; Beçak, W; Stocco, R C

    2015-01-01

    Bovine papillomavirus (BPV) is an oncogenic virus with mucous and epithelial tropism. Possible productive virus infection in other tissues, such as blood, has been hypothesized. In order to investigate this possibility, three samples of skin papillomas and blood were collected from bovines with BPV infection and five samples of peripheral blood and one sample of normal tissue were collected from a calf without BPV infection. Peripheral blood mononuclear cells (PBMCs) were isolated from whole blood and examined by reverse transcription-polymerase chain reaction, immunofluorescence, in situ hybridization, and electron microscopy. The tissue samples were examined for histopathological and immunohistochemical features. The skin papillomas showed the presence of DNA sequences of BPV-2, BPV-11, and a putative virus type. The blood samples showed DNA sequences of BPV-1, 2, and 4 simultaneously. Immunohistochemistry showed BPV L1 protein in both epithelium and stroma and BPV E2 protein in koilocytes. In situ hybridization confirmed the presence of BPV DNA in PBMCs and immunofluorescence showed nuclear labeling of E2 and L1 BPV proteins in PBMCs. The transcription analysis revealed transcripts of BPV-1 L1, BPV-2 L2, and BPV-4 E7 in blood and papilloma samples of BPV-infected cattle. The comet assay revealed high levels of host cell DNA damage upon BPV infection. Electron microscopy analysis of PBMCs identified the presence of particles in the cytoplasm that are consistent with papillomavirus in size and shape. The productive infection of PBMCs with BPV has been previously discussed and this study provides evidence indicating that PBMCs are a target of BPV. PMID:26681018

  18. Chemical composition and biological value of spray dried porcine blood by-products and bone protein hydrolysate for young chickens.

    Science.gov (United States)

    Jamroz, D; Wiliczkiewicz, A; Orda, J; Skorupińska, J; Słupczyńska, M; Kuryszko, J

    2011-10-01

    The chemical composition of spray dried porcine blood by-products is characterised by wide variation in crude protein contents. In spray dried porcine blood plasma (SDBP) it varied between 670-780 g/kg, in spray dried blood cells (SDBC) between 830-930 g/kg, and in bone protein hydrolysate (BPH) in a range of 740-780 g/kg. Compared with fish meal, these feeds are poor in Met and Lys. Moreover, in BPH deep deficits of Met, Cys, Thr and other amino acids were found. The experiment comprised 7 dietary treatments: SDBP, SDBC, and BPH, each at an inclusion rate of 20 or 40 g/kg diet, plus a control. The addition of 20 or 40 g/kg of the analysed meals into feeds for very young chickens (1-28 d post hatch) significantly decreased the body weight (BW) of birds. Only the treatments with 40 g/kg of SDBP and SDBC showed no significant difference in BW as compared with the control. There were no significant differences between treatments and type of meal for feed intake, haematocrit and haemoglobin concentrations in blood. Addition of bone protein and blood cell meals to feed decreased the IgG concentration in blood and caused shortening of the femur and tibia bones. However, changes in the mineral composition of bones were not significantly affected by the type of meal used. The blood by-products, which are rich in microelements, improved retention of Ca and Cu only. In comparison to control chickens, significantly better accretion of these minerals was found in treatments containing 20 g/kg of SDBP or 40 g/kg of SDBC. Great variability in apparent ileal amino acid digestibility in chickens was determined. In this respect, some significant differences related to the type of meal fed were confirmed for Asp, Pro, Val, Tyr and His. In general, the apparent ileal digestibility of amino acids was about 2-3 percentage units better in chickens fed on diets containing the animal by products than in control birds. PMID:22029787

  19. Trigeminal origin or B-preprotachykinin products in feline pial blood vessels

    International Nuclear Information System (INIS)

    A specific and sensitive radioimmunoassay measured the presence of immunoreactive neurokinin A within feline and human pial arteries. Immunoreactivity exhibited a retention time identical to that of synthetic peptide when acid extracts from feline and human blood vessels were subjected to reverse-phase high-performance liquid chromatography. As shown previously for substance P, levels of immunoreactive neurokinin A decreased significantly in the vessels from the ipsilateral rostral circle of Willis l9-24 days following unilateral trigeminal ganglionectomy. Hence, trigeminal projections to cerebral blood vessels contain both products of B-preprotachykinin mRNA. (author)

  20. Prediction of shear stress-related hemolysis in centrifugal blood pumps by computational fluid dynamics

    Institute of Scientific and Technical Information of China (English)

    WANG Fangqun; LI Lan; FENG Zhigang; QIAN Kunxi

    2005-01-01

    A quantitative evaluation of shear stress-related hemolysis in centrifugal blood pumps with different impeller designs has been investigated. Computational fluid dynamics (CFD) is applied to track the shear stress history of the streamlines of red cells. The power law model of the relations among the hemolysis, shear stress and exposure time is used to evaluate the hemolysis in the pumps.Hemolysis tests are also conducted to verify the estimations. Both the estimations and experimentally measured hemolysis levels show that the hemolysis in the streamlined impeller pump developed by the authors is lower than the pump with straight-vane under the same boundary conditions. The approach is proved to be acceptable and practical to predict hemolysis levels of blood pumps.

  1. EFSA NDA Panel (EFSA Panel on Dietetic Products, Nutrition and Allergies), 2014. Scientific Opinion on the substantiation of a health claim related to “Lactobacillus plantarum TENSIA® in the semi-hard Edam-type „heart cheese‟ of Harmony™” and maintenance of normal blood pressure pursuant to Article

    DEFF Research Database (Denmark)

    Tetens, Inge

    scientific substantiation of the claim. Two human randomised, double-blind, placebo-controlled, intervention studies investigated the effect on BP of L. plantarum TENSIA® consumed in Edam-type cheese for three and eight weeks. In weighing the evidence, the Panel took into account that only one human...... the scientific substantiation of a health claim related to “Lactobacillus plantarum TENSIA® in the semi-hard Edam-type “heart cheese” of Harmony™” and maintenance of normal blood pressure (BP). The food constituent L. plantarum TENSIA®, which is the subject of the health claim, is sufficiently...

  2. Association between religiousness and blood donation among Brazilian postgraduate students from health-related areas

    OpenAIRE

    Edson Zangiacomi Martinez; Rodrigo Guimarães dos Santos Almeida; Ana Carolina Garcia Braz; Antonio Carlos Duarte de Carvalho

    2014-01-01

    OBJECTIVE: The aim of this study was to examine the association between religiousness and blood donation among postgraduate students.METHODS: The Portuguese-language version of the Duke University Religion Index was administered to a sample of 226 Brazilian students with ages ranging from 22 to 55 years. All study participants had completed undergraduate courses in health-related areas.RESULTS: In the present study, 23.5% of the students were regular donors. Organizational religiousness was f...

  3. Influence of Relative Humidity on the Spreading Dynamics of a Drying Drop of Whole Blood

    Science.gov (United States)

    Bou Zeid, Wassim; Brutin, David

    2013-11-01

    Newtonian and non-Newtonian fluids start spreading after coming into contact with a solid substrate till the anchoring of the triple line. Our experimental work aims to study the effect of the relative humidity on the spreading dynamics of drops of whole blood. Drops of blood of same volume (+/-4.8%) are injected using a digital micropipette and gently deposited onto microscope ultraclean glass substrates. Experiments are conducted in a glove box at ambient temperature and a range of investigated relative humidities between 13.0% and 80.0%. The recorded images are post-processed using ImageJ in which the position of the contact line is measured every 20 ms. We show that the spreading dynamics is, in a first time, governed by relative humidity and later no more influence by relative humidity. Two spreading regimes are observed and analyzed compared to classical viscous drops. In previous work, we show that relative humidity influences the contact angle and the initial wetting radius. In the first regime, we find a spreading power law exponent that decreases for an increasing relative humidity. In the second regime, all the data collapse on each other and the evolution of the dimensionless radius no more depend on relative humidity.

  4. Whole Blood RNA as a Source of Transcript-Based Nutrition- and Metabolic Health-Related Biomarkers

    Science.gov (United States)

    Petrov, Petar D.; Bonet, M. Luisa; Reynés, Bárbara; Oliver, Paula; Palou, Andreu; Ribot, Joan

    2016-01-01

    Blood cells are receiving an increasing attention as an easily accessible source of transcript-based biomarkers. We studied the feasibility of using mouse whole blood RNA in this context. Several paradigms were studied: (i) metabolism-related transcripts known to be affected in rat tissues and peripheral blood mononuclear cells (PBMC) by fasting and upon the development of high fat diet (HFD)-induced overweight were assessed in whole blood RNA of fasted rats and mice and of HFD-fed mice; (ii) retinoic acid (RA)-responsive genes in tissues were assessed in whole blood RNA of control and RA-treated mice; (iii) lipid metabolism-related transcripts previously identified in PBMC as potential biomarkers of metabolic health in a rat model were assessed in whole blood in an independent model, namely retinoblastoma haploinsufficient (Rb+/-) mice. Blood was collected and stored in RNAlater® at -80°C until analysis of selected transcripts by real-time RT-PCR. Comparable changes with fasting were detected in the expression of lipid metabolism-related genes when RNA from either PBMC or whole blood of rats or mice was used. HFD-induced excess body weight and fat mass associated with expected changes in the expression of metabolism-related genes in whole blood of mice. Changes in gene expression in whole blood of RA-treated mice reproduced known transcriptional actions of RA in hepatocytes and adipocytes. Reduced expression of Fasn, Lrp1, Rxrb and Sorl1 could be validated as early biomarkers of metabolic health in young Rb+/- mice using whole blood RNA. Altogether, these results support the use of whole blood RNA in studies aimed at identifying blood transcript-based biomarkers of nutritional/metabolic status or metabolic health. Results also support reduced expression of Fasn, Lrp1, Rxrb and Sorl1 in blood cells at young age as potential biomarkers of metabolic robustness. PMID:27163124

  5. Postulated Role of Vasoactive Neuropeptide-Related Immunopathology of the Blood Brain Barrier and Virchow-Robin Spaces in the Aetiology of Neurological-Related Conditions

    Directory of Open Access Journals (Sweden)

    D. R. Staines

    2008-01-01

    Full Text Available Vasoactive neuropeptides (VNs such as pituitary adenylate cyclase-activating polypeptide (PACAP and vasoactive intestinal peptide (VIP have critical roles as neurotransmitters, vasodilators including perfusion and hypoxia regulators, as well as immune and nociception modulators. They have key roles in blood vessels in the central nervous system (CNS including maintaining functional integrity of the blood brain barrier (BBB and blood spinal barrier (BSB. VNs are potent activators of adenylate cyclase and thus also have a key role in cyclic AMP production affecting regulatory T cell and other immune functions. Virchow-Robin spaces (VRSs are perivascular compartments surrounding small vessels within the CNS and contain VNs. Autoimmunity of VNs or VN receptors may affect BBB and VRS function and, therefore, may contribute to the aetiology of neurological-related conditions including multiple sclerosis, Parkinson's disease, and amyotrophic lateral sclerosis. VN autoimmunity will likely affect CNS and immunological homeostasis. Various pharmacological and immunological treatments including phosphodiesterase inhibitors and plasmapheresis may be indicated.

  6. Use of paravascular admittance waveforms to monitor relative change in arterial blood pressure

    Science.gov (United States)

    Zielinski, Todd M.; Hettrick, Doug; Cho, Yong

    2010-04-01

    Non-invasive methods to monitor ambulatory blood pressure often have limitations that can affect measurement accuracy and patient adherence [1]. Minimally invasive measurement of a relative blood pressure surrogate with an implantable device may provide a useful chronic diagnostic and monitoring tool. We assessed a technique that uses electrocardiogram and paravascular admittance waveform morphology analysis to one, measure a time duration (vascular tone index, VTI in milliseconds) change from the electrocardiogram R-wave to admittance waveform peak and two, measure the admittance waveform minimum, maximum and magnitude as indicators of change in arterial compliance/distensibility or pulse pressure secondary to change in afterload. Methods: Five anesthetized domestic pigs (32 ± 4.2 kg) were used to study the effects of phenylephrine (1-5 ug/kg/min) on femoral artery pressure and admittance waveform morphology measured with a quadrapolar electrode array catheter placed next to the femoral artery to assess the relative change in arterial compliance due to change in peripheral vascular tone. Results: Statistical difference was observed (p measurement techniques that monitor relative change in blood pressure may be suitable for implantable devices to detect progression of cardiovascular disease such as hypertension.

  7. Cerebral blood flow velocities after subarachnoid haemorrhage in relation to the amount of blood clots in the initial computed tomography

    International Nuclear Information System (INIS)

    In 72 patients with acute subarachnoid haemorrhage (SAH) the relationship between the amount of subarachnoid blood clots detected by initial cranial computed tomography (CCT) up to 48 hours after bleeding and the later development of vasospasm, established by blood flow velocity measurement with transcranial Doppler ultrasound (TCD) was investigated. The serial Doppler examinations started within the first 72 hours after SAH and were carried out every second day up to three weeks. Each Doppler recording was accompanied by a neurological examination. Patients classified as Hunt and Hess grade V were excluded from the study. All patients with remarkable brain oedema in CCT or with intracranial pressure above 25 mmHg were also excluded. Because of the well known agedependence of vasospasm after SAH, two age groups were formed. A statistically significant correlation (p > 0.05) between blood flow velocities and blood load after SAH was not found. The mean age of the investigated 72 individuals was 48.9 years (14 up to 76 years). 47 patients were younger than 56 years. Linear regression analysis indicated a correlation with a quite low significance level (r = 0.350, p 0.05) between these two variables could be established in the 25 patients older than 55 years. In a second step an intra-individual comparison of side-to-side differences in TCD and CCT was made. There were no significant differences in blood flow velocities between subjects with or without side-to-side differences in cisternal blood load. It is concluded that the amount of blood visible on initial CCT after SAH is not a powerful predictor of cerebral blood flow velocities measured by TCD. (author)

  8. Percentile distribution of blood pressure readings in relation to body mass index: a populationbased cross-sectional study ADOPOLNOR

    Directory of Open Access Journals (Sweden)

    Krzyżaniak Alicja

    2015-03-01

    Full Text Available Recent upward trends toward elevated blood pressure and increased weight expressed in terms of body mass index in children and adolescents call for regular monitoring of their physical growth and age-related changes in blood pressure. This requires adequate tools - reference values of a normal blood pressure range. The main objective of this study was to provide sex- and BMI-specific percentile reference values for systolic and diastolic blood pressure based on the adolescent Polish population, participants in the ADOPOLNOR study. A cross-sectional survey was carried out on a representative, randomly selected cohort of 4,941; 2,451 male and 2,490 female students aged 10-18 years, residents in Wielkopolska province and its capital, the city of Poznań. All examinations were performed in school nursery rooms during morning hours according to standard procedures. Body height and weight were measured and BMI was calculated. Blood pressure was measured twice on each occasion on the right arm using a fully calibrated TECH MED TM-Z mercury gauge sphygmomanometer with sets of exchangeable cuffs and a clinical stethoscope. The blood pressure classification was determined using the surveillance method. For each participant, the mean of measurements taken on each of the three occasions was calculated and served as his/her final blood pressure value. Using the LMS method, fitted percentile curves were created for BMI-related systolic and diastolic blood pressure. The findings revealed that age related blood pressure pattern was similar in boys and girls. It showed a steady increase of systolic and diastolic blood pressure with age. There was a positive correlation between the systolic and diastolic blood pressure indicating that when systolic blood pressure increased so diastolic did (r=0.61 at p<0.01. Boys were likely to have relatively higher mean values of systolic and diastolic blood pressure and steeper slope for BMI-related change in blood pressure

  9. Dry period cooling ameliorates physiological variables and blood acid base balance, improving milk production in murrah buffaloes

    Science.gov (United States)

    Aarif, Ovais; Aggarwal, Anjali

    2016-03-01

    This study aimed to evaluate the impact of evaporative cooling during late gestation on physiological responses, blood gas and acid base balance and subsequent milk production of Murrah buffaloes. To investigate this study sixteen healthy pregnant dry Murrah buffaloes (second to fourth parity) at sixty days prepartum were selected in the months of May to June and divided into two groups of eight animals each. One group of buffaloes (Cooled/CL) was managed under fan and mist cooling system during dry period. Group second buffaloes (Noncooled/NCL) remained as control without provision of cooling during dry period. The physiological responses viz. Rectal temperature (RT), Respiratory rate (RR) and Pulse rate were significantly ( P < 0.05) lower in group 2, with the provision of cooling. Skin surface temperature at thorax was significantly lower in cooled group relative to noncooled group. Blood pH and pO2 were significantly ( P < 0.05) higher in heat stressed group as compared to the cooled group. pCO2, TCO2, HCO3, SBC, base excess in extracellular fluid (BEecf), base excess in blood (BEb), PCV and Hb were significantly ( P < 0.05) higher in cooled group as compared to noncooled group. DMI was significantly ( P < 0.05) higher in cooled relative to noncooled animals. Milk yield, FCM, fat yield, lactose yield and total solid yield was significantly higher ( P < 0.05) in cooled group of Murrah buffaloes.

  10. Particle Pair Production in Cosmological General Relativity

    OpenAIRE

    Oliveira, Firmin J.

    2012-01-01

    The Cosmological General Relativity (CGR) of Carmeli, a 5-dimensional (5-D) theory of time, space and velocity, predicts the existence of an acceleration a_0 = c / tau due to the expansion of the universe, where c is the speed of light in vacuum, tau = 1 / h is the Hubble-Carmeli time constant, where h is the Hubble constant at zero distance and no gravity. The Carmeli force on a particle of mass m is F_c = m a_0, a fifth force in nature. In CGR, the effective mass density rho_eff = rho - rho...

  11. Development of a laser speckle imaging system for measuring relative blood flow velocity

    Science.gov (United States)

    Smith, Michael S. D.; Packulak, Ernie F.; Sowa, Michael G.

    2006-09-01

    Determining the viability of damaged or surgically reconstructed tissue is critical in most plastic and reconstructive surgery procedures. Information about tissue blood flow in the region in question can make this determination much easier. Laser speckle imaging (LSI) is one technique that could potentially aid in making this determination. LSI is a non-contact full-field imaging technique with simultaneous high spatial and temporal resolution. Tissue is illuminated with diffuse red laser light and the spatial and/or temporal statistics of the resulting speckle pattern can be used to calculate relative flow velocities. We have developed a LSI system that produces relative velocity blood flow images. Bench tests of the system indicate that it may be used to distinguish between normal, decreased, and increased blood flow states of a human finger. The system has also been used to take some initial laboratory measurements using an animal model - an epigastric free flap on a rat. Preliminary results indicate that the method may be used to distinguish states of venous or arterial occlusion from unoccluded states of the skin flap. While further experimentation is necessary, these initial results indicate that LSI could be a useful aid to the plastic surgeon for assessing tissue viability.

  12. Salivary latent trait cortisol (LTC): Relation to lipids, blood pressure, and body composition in middle childhood.

    Science.gov (United States)

    Yeung, Ellen W; Place, Rebecca; Gordish-Dressman, Heather; Visich, Paul; Hoffman, Eric; Walker, Sheila O; Granger, Douglas A

    2016-09-01

    Adversity experienced early in life has the potential to influence physical health later in life. The stress-health relation may be partially explained by stress-related effects on cardiovascular risk factors. This study explored links between individual differences in trait-like variation in the activity of the hypothalamic-pituitary-adrenal (HPA) axis with cardiovascular risk factors in children. 474 children (M age=9.22years; 54% female; 83% Caucasian) were included in this study, in which cardiovascular risk was assessed using the following indices - triglycerides (TG), HDL-cholesterol (HDL-C), glucose (Glu); resting systolic and diastolic blood pressure, body mass index (BMI), waist-to-hip ratio, and % fat. Saliva samples were measured 3 times a day (waking, 30min post-waking and bedtime) over 3days (later assayed for cortisol). A latent trait cortisol (LTC) factor explained 43% of the variance in cortisol levels within and across days. Confirmatory factor analysis identified three cardiovascular risk factors: lipids (i.e., TG and HDL-C), blood pressure (i.e., systolic and diastolic), and body composition (i.e., BMI, Waist-to-hip ratio, and % fat). Lower salivary LTC was associated with higher lipids, higher blood pressure, and higher body composition. The findings further support the internal and external validity of the LTC construct, and may also advance our understanding of the link between interindividual differences in HPA axis activity and cardiovascular risk in middle childhood. PMID:27262343

  13. Production of fibrogenic cytokines by interleukin-2-treated peripheral blood leukocytes

    DEFF Research Database (Denmark)

    Kovacs, E J; Brock, B; Silber, I E;

    1993-01-01

    procollagen and fibronectin messenger RNAs was increased in human fibroblasts in response to leukocyte supernatants. Unstimulated leukocytes expressed minimal levels of transforming growth factor-beta or platelet-derived growth factor B chain messenger RNAs, but could be greatly enhanced by IL-2 treatment....... CONCLUSION: Mediators that induce connective tissue production are secreted by IL-2-treated peripheral blood leukocytes. These cytokines may be responsible, in part, for the stimulation of abdominal adhesions in patients receiving intraperitoneal immunotherapy....

  14. The relative efficacy of telephone and email reminders to elicit blood donation.

    Science.gov (United States)

    Germain, M; Godin, G

    2016-04-01

    The aim of the study was to test the relative efficacy of telephone and email reminders to trigger blood donation. A sample of 3454 donors was randomized to one of three conditions: phone only (n = 1176), email only (n = 1091) and phone + email (n = 1187). There was a higher proportion of donors who registered to give blood in the phone + email condition (18·45%) compared to the other two conditions (phone: 15·73%, P < 0·05; email: 13·20%; P < 0·001); this effect was apparent only in men. The phone and email conditions did not differ significantly (P = 0·16), suggesting equivalent efficacy. PMID:26198382

  15. CYTOKINES IN LACRIMAL FLUID AND BLOOD SERUM: EARLY BIOMARKERS OF AGE-RELATED MACULAR DEGENERATION

    Directory of Open Access Journals (Sweden)

    O. S. Slepova

    2015-06-01

    Full Text Available The article presents results of multiplex cytokine assays in blood serum and lacrimal fluid at the initial and intermediate stages of age-related macular degeneration (AMD. Some features of local and systemic disturbances in the cytokine profile were detected in these patients. It was revealed that the initial stage of AMD was associated with elevated IL-17 levels in lacrimal fluid, along with imbalance between the local increase and systemic decrease of TGF-β1 amounts. Intermediate-stage AMD was associated with increased levels of the most cytokines assayed (except of TGF-β1 in blood serum and lacrimal fluid, thus suggesting stimulation of both pro-inflammatory and angiogenic responses, like as activation of anti-inflammatory and anti-infective factors. 

  16. Establishment and performance assessment of preparation technology of internal quality control products for blood transfusion compatibility testing

    OpenAIRE

    Yu, Yang; MA, CHUNYA; Feng, Qian; Chen, Xin; GUAN, XIAOZHEN; Zhang, Xiaojuan; Chen, Linfeng; Lin, Zilin; Pan, Jichun; Zhang, Ting; Luo, Qun; Wang, Deqing

    2013-01-01

    The aim of this study was to establish and to optimize the preparation technology of whole blood internal quality control (IQC) products for blood transfusion compatibility testing. Several B-type RhD-negative blood samples collected from healthy donors were mixed. Two groups of whole blood IQC products, namely, the preservative solution group (PS group) and the saline group, were prepared. The agglutination intensity of IQC sample red cells and anti-B antibody, IgM anti-A antibody and revers...

  17. An oat bran meal influences blood insulin levels and related gene sets in peripheral blood mononuclear cells of healthy subjects

    OpenAIRE

    Ulmius, Matilda; Johansson-Persson, Anna; Krogh, Morten; Olsson, Peter; Önning, Gunilla

    2011-01-01

    The understanding of how fibre-rich meals regulate molecular events at a gene level is limited. This pilot study aimed to investigate changes in gene expression in peripheral blood mononuclear cells (PBMCs) from healthy subjects after consumption of an oat bran-rich meal. Fifteen subjects (8 men and 7 women, aged 20–28 years) ingested meals with oat bran or a control meal after an overnight fast. Blood samples for analysis of postprandial glucose, insulin and triglyceride concentrations were ...

  18. Left ventricular hypertrophy in relation to systolic blood pressure and the angiotensin converting enzyme I/D polymorphism in Chinese

    Institute of Scientific and Technical Information of China (English)

    Alexander P. Headley; Yan Li; Yi Zhang; Ji-Yong Ge; Qi-Fang Huang; Ji-Guang Wang

    2009-01-01

    Objective There is little population-based data on the prevalence and the environmental or genetic determinants of left ventricular hypertrophy (LVH) in China. The purpose of this paper is to study LVH in relation to systolic blood pressure and the angiotensin converting enzyme (ACE) insertion/deletion(I/D) polymorphism in Chinese. Methods We recorded 12-lead ECG (CardioSoft, v4.2) in 1365 residents in the Jingning County, Zhejiang Province, China. LVH was defined according to the gender-specific Sokolow-Lyon and Comell product ECG criteria. Results Regardless of whether the Sokolow-Lyon or Comell product ECG criteria was used, the prevalence of LVH (20.7% and 4.8%, respectively) significantly (P<0.0001) increased with male gender (odds ratio [OR] 2.33 and 7.15) and systolic blood pressure (per 10 mm Hg increase, OR 1.46 and 1.33). If the Sokolow-Lyon criteria was used, the prevalence of LVH was also influenced by alcohol intake (OR 1.44, P=0.03) and body mass index (OR 0.83, P=0.0005). The association between the Sokolow-Lyon voltage amplitude and the ACE I/D polymorphism was dependent on antihypertensive therapy (P=0.01). In 1262 untreated subjects, but not 103 patients on antihypertensive medication, the ACE DD compared with Ⅱ subjects had significantly higher Sokolow-Lyon voltage amplitudes (29.8±0.6 vs. 28.0±20.5 mV, P=0.02) and higher risk of LVH (OR 1.74, 95% CI: 1. 12-2.69, P=0.01). Conclusion LVH is prevalent in Chinese, and is associated with systolic blood pressure and the ACE D allele. The genetic association might be modulated by antihypertensive therapy.

  19. Complications related to blood donation: A multicenter study of the prevalence and influencing factors in voluntary blood donation camps in Karnataka, India

    Science.gov (United States)

    Agarwal, Rajat Kumar; Periyavan, Sundar; Dhanya, Rakesh; Parmar, Lalith G.; Sedai, Amit; Ankita, Kumari; Vaish, Arpit; Sharma, Ritesh; Gowda, Prabha

    2016-01-01

    Introduction: Complications associated with blood donation significantly lower odds of subsequent donations. The aim of the study is to assess the prevalence of complications related to blood donation, identify the influencing factors, and come up with suggestions for minimizing discomfort to donors and making outdoor voluntary blood donation camps safer. Materials and Methods: This study covered 181 blood donation camps organized by Sankalp India Foundation where 16 blood banks participated from 01-04-2011 to 01-08-2014 in Karnataka. Uniform protocols for donor selection, predonation preparation, counseling, postdonation care, and refreshments were used. The postdonation complications were recorded on a form immediately, after they were observed. Results: We observed 995 (3.2%) complications in 30,928 whole blood donations. Of these 884 (2.86%) mild, 77 (0.25%) moderate, and 5 (0.02%) severe complications were observed. Local symptoms (blood outside vessels, pain, and allergy) contributed 1.0%, and generalized symptoms (vasovagal reaction) contributed 2.2% to all the complications. Conclusion: We observed 322 complications for every 10,000 donations. Since 27 out of every 10000 experience moderate and severe complication, the readiness to manage complications is crucial. Women donors, young donors, and donors with a lower weight are at a significantly greater risk of experiencing complications, highlighting the need for specific guidelines for the management of higher risk donor groups. Complications varied significantly between various blood banks. Predonation hydration was effective in limiting complications with generalized symptoms. We recommend a robust donor hemovigilance program for voluntary blood donation for monitoring complications and enable assessment of effectiveness and implementation of appropriate interventions. PMID:27011671

  20. Complications related to blood donation: A multicenter study of the prevalence and influencing factors in voluntary blood donation camps in Karnataka, India

    Directory of Open Access Journals (Sweden)

    Rajat Kumar Agarwal

    2016-01-01

    Full Text Available Introduction: Complications associated with blood donation significantly lower odds of subsequent donations. The aim of the study is to assess the prevalence of complications related to blood donation, identify the influencing factors, and come up with suggestions for minimizing discomfort to donors and making outdoor voluntary blood donation camps safer. Materials and Methods: This study covered 181 blood donation camps organized by Sankalp India Foundation where 16 blood banks participated from 01-04-2011 to 01-08-2014 in Karnataka. Uniform protocols for donor selection, predonation preparation, counseling, postdonation care, and refreshments were used. The postdonation complications were recorded on a form immediately, after they were observed. Results: We observed 995 (3.2% complications in 30,928 whole blood donations. Of these 884 (2.86% mild, 77 (0.25% moderate, and 5 (0.02% severe complications were observed. Local symptoms (blood outside vessels, pain, and allergy contributed 1.0%, and generalized symptoms (vasovagal reaction contributed 2.2% to all the complications. Conclusion: We observed 322 complications for every 10,000 donations. Since 27 out of every 10000 experience moderate and severe complication, the readiness to manage complications is crucial. Women donors, young donors, and donors with a lower weight are at a significantly greater risk of experiencing complications, highlighting the need for specific guidelines for the management of higher risk donor groups. Complications varied significantly between various blood banks. Predonation hydration was effective in limiting complications with generalized symptoms. We recommend a robust donor hemovigilance program for voluntary blood donation for monitoring complications and enable assessment of effectiveness and implementation of appropriate interventions.

  1. Banking of pluripotent adult stem cells as an unlimited source for red blood cell production: potential applications for alloimmunized patients and rare blood challenges.

    Science.gov (United States)

    Peyrard, Thierry; Bardiaux, Laurent; Krause, Claire; Kobari, Ladan; Lapillonne, Hélène; Andreu, Georges; Douay, Luc

    2011-07-01

    The transfusion of red blood cells (RBCs) is now considered a well-settled and essential therapy. However, some difficulties and constraints still occur, such as long-term blood product shortage, blood donor population aging, known and yet unknown transfusion-transmitted infectious agents, growing cost of the transfusion supply chain management, and the inescapable blood group polymorphism barrier. Red blood cells can be now cultured in vitro from human hematopoietic, human embryonic, or human-induced pluripotent stem cells (hiPSCs). The highly promising hiPSC technology represents a potentially unlimited source of RBCs and opens the door to the revolutionary development of a new generation of allogeneic transfusion products. Assuming that in vitro large-scale cultured RBC production efficiently operates in the near future, we draw here some futuristic but realistic scenarios regarding potential applications for alloimmunized patients and those with a rare blood group. We retrospectively studied a cohort of 16,486 consecutive alloimmunized patients (10-year period), showing 1 to 7 alloantibodies with 361 different antibody combinations. We showed that only 3 hiPSC clones would be sufficient to match more than 99% of the 16,486 patients in need of RBC transfusions. The study of the French National Registry of People with a Rare Blood Phenotype/Genotype (10-year period) shows that 15 hiPSC clones would cover 100% of the needs in patients of white ancestry. In addition, one single hiPSC clone would meet 73% of the needs in alloimmunized patients with sickle cell disease for whom rare cryopreserved RBC units were required. As a result, we consider that a very limited number of RBC clones would be able to not only provide for the need for most alloimmunized patients and those with a rare blood group but also efficiently allow for a policy for alloimmunization prevention in multiply transfused patients. PMID:21377319

  2. Blood Collection from Local Abattoirs for Mass Production of Tsetse Flies to be used in the Sterile Insect Technique

    International Nuclear Information System (INIS)

    The mass production of tsetse flies (Glossina spp.) for the sterile insect technique (SIT) requires a supply of quality blood. For some years already cattle blood has been used as food for laboratory reared flies. The blood is collected from an abattoir using standard procedures. The collection procedures, handling and storage require aseptic conditions to avoid contamination of the blood, which could be fatal to the flies. Fly mortality caused by low quality blood endangers the success of mass rearing. To rear healthy flies with good survival and production the blood should be of good quality - free of contamination and with a packed cell volume (PCV) above 25%. The present work involved the seasonal collection of blood from abattoirs in the United Republic of Tanzania (Tanga, Arusha, Dodoma, Dar-es-salaam). Dodoma was identified as having the best conditions for blood collection. To assess the quality of the blood as a diet for tsetse, blood was screened for the presence of bacteria, and the pathogens were identified. Protocols were developed for blood quality assurance. (author)

  3. Production of Autoantibodies in Chronic Hepatitis B Virus Infection Is Associated with the Augmented Function of Blood CXCR5+CD4+ T Cells.

    Science.gov (United States)

    Lei, Yu; Hu, Tingting; Song, Xiaofei; Nie, Hong; Chen, Min; Chen, Weixian; Zhou, Zhi; Zhang, Dazhi; Hu, Huaidong; Hu, Peng; Ren, Hong

    2016-01-01

    T follicular helper cells (Tfh) provide help to B cells to support their activation, expansion and differentiation. However, the role of Tfh cells in chronic HBV infection is poorly defined. The aim of this research was to examine the function of Tfh cells and whether they are involved in HBV related disease. Blood CXCR5+CD4+T cells and B cells in 85 patients with chronic HBV infection (HBV patients) and health controls (HC) were examined by flow cytometry. The molecule expression in blood CXCR5+CD4+ T cells was detected by real-time PCR. Blood CXCR5+CD4+ T cells and B cells were co-cultured and the production of Ig and cytokines was detected by ELISA. Autoantibodies were detected by indirect immunofluorescence and immunospot assay. We found that blood CXCR5+CD4+ T cells in patients with chronic HBV infection (HBV patients) expressed higher level of activation related molecules and cytokines than that from health controls (HC).In HBV patients, the frequency of blood CXCR5+CD4+ T cells was significantly correlated with serum ALT and AST. We also found that blood CXCR5+CD4+ T cells from HBV patients could induce B cells to secret higher level of immunoglobulin than that from HC. Several autoantibodies, including ANA, ss-A, ss-B, Scl-70, Jo-1, ect, were indeed positive in 65% HBV patients. Among HBV patients, expression of function related molecules was significantly higher in blood CXCR5+CD4+ T cells from patients with autoantibodies than that without autoantibodies. Our research indicated that blood CXCR5+CD4+ T cells from HBV patients were over activated and show augmented capacity to help B cells for antibody secreting, which might correlated with liver inflammation and the production of autoantibodies in extrahepatic manifestations. PMID:27612199

  4. Hydrocortisone sodium succinate suppressed production of interleukin-10 by human peripheral blood mononuclear cells: clinical significance.

    Directory of Open Access Journals (Sweden)

    Kohka H

    1999-02-01

    Full Text Available Corticoids are well known for their immunosuppressive properties. Interleukin-10 (IL-10 is an intrinsic antiinflammatory peptide in immune diseases, originally identified as cytokine synthesis inhibitory factor. We examined the effect of hydrocortisone sodium succinate (HSS on the production of IL-10 by human peripheral blood mononuclear cells (PBMCs. PBMCs from healthy volunteers and cancer-burden patients were preincubated separately with or without HSS for 1 h, then stimulated with 5 microg/ml lipopolysaccharide (LPS. Production of IL-10 by human PBMCs was detected with LPS stimulation and its production was higher in cancer-burden patients than in normal volunteers, although this was not statistically significant. HSS suppressed production of IL-10 by LPS-stimulated PBMCs in a dose-dependent manner both in normal volunteers and in cancer-burden patients. These results indicate that, in addition to their antiinflammatory properties, corticoids act to restore the immunosuppressive states even in cancer-burden states.

  5. Relation between ABO blood groups and Helicobacter pylori infection in symptomatic patients

    Directory of Open Access Journals (Sweden)

    Jaff MS

    2011-09-01

    Full Text Available Mohamad Salih Jaff Pathology Department, College of Medicine, Hawler Medical University (formerly Salahuddin University, Erbil, Kurdistan Region, Iraq Abstract: Epidemiological studies have demonstrated higher frequencies of the O blood group and the nonsecretor phenotype of ABH antigens among patients suffering from peptic ulcers. Since Helicobacter pylori has been established as the main etiological factor in this disease, controversies about the associations of the ABO and Lewis blood group phenotypes and secretor and nonsecretor phenotypes in relation to susceptibility towards infection by this bacillus have been presented. The aim of this study was to verify the frequencies of ABO and Rhesus (Rh blood groups in H. pylori seropositive symptomatic patients. The study included (n = 1108 patients with dyspepsia symptoms referred from an outpatient clinic in Erbil city for investigation. Age, sex, and residency were recorded as a routine laboratory framework. Patients underwent SD Bioline (Standard Diagnostics Inc, Kyonggi-do, South Korea and enzyme-linked immunosorbent assay serologic tests for H. pylori. ABO blood group phenotypes were determined by a standard hemagglutination test. Results showed that 64.8% of patients (n = 718/1108 were seropositive for H. pylori infection, and (35.2% (n = 390/1108 were seronegative. Of the seropositive patients, 40.8% (n = 293/718 were male and 59.2% (n = 425/718 were female; while of the seronegative patients, 46.7% (n = 182/390 were male and 53.3% (n = 208/390 were female. The mean age for seropositives and seronegatives was (38.0 ± 14.6 years and (37.6 ± 15.7 years respectively. The frequency of the ABO and Rh-positive (Rh+ blood groups among seropositive patients was (A = 32.0%, B = 19.5%, AB = 6.7%, O = 41.8%, and Rh+ = 92.5% and was (A = 32.3%, B = 28.2%, AB = 8.0%, O = 31.5%, and Rh+ = 92.5% in seronegatives. The results of this study suggest that ABO blood groups, age, and gender influence

  6. Do blood components affect the production of reactive oxygen species (ROS by equine synovial cells in vitro?

    Directory of Open Access Journals (Sweden)

    Patrícia M. Brossi

    2012-12-01

    Full Text Available Blood-derived products are commonly administered to horses and humans to treat many musculoskeletal diseases, due to their potential antioxidant and anti-inflammatory effects. Nevertheless, antioxidant effects have never been shown upon horse synovial fluid cells in vitro. If proved, this could give a new perspective to justify the clinical application of blood-derived products. The aim of the present study was to investigate the antioxidant effects of two blood-derived products - plasma (unconditioned blood product - UBP and a commercial blood preparation (conditioned blood product - CBP¹ - upon stimulated equine synovial fluid cells. Healthy tarsocrural joints (60 were tapped to obtain synovial fluid cells; these cells were pooled, processed, stimulated with lipopolysaccharide (LPS or phorbol 12-myristate 13-acetate (PMA, and evaluated by flow cytometry for the production of reactive oxygen species (ROS. Upon addition of any blood-derived product here used - UBP and CBP - there was a significant decrease in the oxidative burst of synovial fluid cells (P<0.05. There was no difference between UBP and CBP effects. In conclusion, treatment of stimulated equine synovial cells with either UBP or CBP efficiently restored their redox equilibrium.

  7. Monitoring of milk production and total cholesterol concentration, gamma-glutamyltransferase, and glutathione peroxidase in Simmental cows blood

    Directory of Open Access Journals (Sweden)

    Terezija Silvija Marenjak

    2007-06-01

    Full Text Available Milk production, total blood cholesterol concentration and activity ofgamma-glutamyl-transferase (GGT and glutation peroxidase (GPx in blood of Simmental cows was monitored during two control periods (first control period - May 2004; second control period - June 2004. The objective was to determine a relationship between milk production and particular blood parameters, and their possible implementation as an indicator of milk production. Twelve Simmental cows from small-scale dairy farm in Zagreb County were included in the study. Cows originated from the same herd andwere roughly of the same parity and stage of lactation. The average milk production and total blood cholesterol concentration were significantly higher in May then in June (20,55 L/d vs. 15,44 L/d; 4,55 mmol/L vs. 3,96 mmol/L, respectively, whereas the GPx and GGT activity was significantly lower in May in comparison with June (GPx=1720,66 U/L vs. GPx=1808 U/L, and GGT = 20,11 U/L vs. 23,22 U/L, respectively. A positive correlation between the milk production and the total blood cholesterol level was detected (r=0,58. The total blood cholesterol concentration in the blood plasma mighthave been one of the indicators of production performance in the Simmental cows herd, whereas the activity of stated enzymes may specify the nutritional status thereof the milk production depends on.

  8. Xenotropic Murine Leukemia Virus-Related Virus (XMRV) and the Safety of the Blood Supply.

    Science.gov (United States)

    Johnson, Andrew D; Cohn, Claudia S

    2016-10-01

    In 2006, a new virus, xenotropic murine leukemia virus-related virus (XMRV), was discovered in a cohort of U.S. men with prostate cancer. Soon after this initial finding, XMRV was also detected in samples from patients with chronic fatigue syndrome (CFS). The blood community, which is highly sensitive to the threat of emerging infectious diseases since the HIV/AIDS crisis, recommended indefinite deferral of all blood donors with a history of CFS. As XMRV research progressed, conflicting results emerged regarding the importance of this virus in the pathophysiology of prostate cancer and/or CFS. Molecular biologists traced the development of XMRV to a recombination event in a laboratory mouse that likely occurred circa 1993. The virus was propagated via cell lines derived from a tumor present in this mouse and spread through contamination of laboratory samples. Well-controlled experiments showed that detection of XMRV was due to contaminated samples and was not a marker of or a causal factor in prostate cancer or CFS. This paper traces the development of XMRV in the prostate and CFS scientific communities and explores the effect it had on the blood community. PMID:27358491

  9. Interactions of Several Lipid-Related Gene Polymorphisms and Cigarette Smoking on Blood Pressure Levels

    Directory of Open Access Journals (Sweden)

    Rui-Xing Yin, Dong-Feng Wu, Jin-Zhen Wu, Xiao-Li Cao, Lynn Htet Htet Aung, Lin Miao, Xing-Jiang Long, Wan-Ying Liu, Lin Zhang, Meng Li

    2012-01-01

    Full Text Available The interactions of single nucleotide polymorphisms (SNPs and cigarette smoking on blood pressure levels are limited. The present study was undertaken to detect nine lipid-related SNPs and their interactions with cigarette smoking on blood pressure levels. Genotyping of ATP-binding cassette transporter A1 (ABCA-1 V825I, acyl-CoA:cholesterol acyltransferase-1 (ACAT-1 rs1044925, low density lipoprotein receptor (LDL-R AvaⅡ, hepatic lipase gene (LIPC -250G>A, endothelial lipase gene (LIPG 584C>T, methylenetetrahydrofolate reductase (MTHFR 677C>T, proprotein convertase subtilisin-like kexin type 9 (PCSK9 E670G, peroxisome proliferator-activated receptor delta (PPARD +294T>C, and Scavenger receptor class B type 1 (SCARB1 rs5888 was performed in 935 nonsmokers and 845 smokers. The interactions were detected by factorial regression analysis. The frequencies of genotypes (ACAT-1 and LIPG, alleles (ABCA-1, and both genotypes and alleles (LDL-R, LIPC, PPARD and SCARB1 were different between nonsmokers and smokers (P < 0.05-0.001. The levels of pulse pressure (PP, ABCA-1, and systolic, diastolic blood pressure (SBP, DBP and PP (LIPC in nonsmokers were different among the genotypes (P < 0.01-0.001. The levels of SBP (ABCA-1, ACAT-1, LIPG and PCSK9, DBP (ACAT-1, LDL-R, LIPC, PCSK9 and PPARD, and PP (LIPC, LIPG, MTHFR and PCSK9 in smokers were different among the genotypes (P < 0.01-0.001. The SNPs of ABCA-1, ACAT-1 and PCSK9; ACAT-1, LDL-R, MTHFR and PCSK9; and ABCA-1, LIPC, PCSK9 and PPARD were shown interactions with cigarette smoking to influence SBP, DBP and PP levels (P < 0.05-0.001; respectively. The differences in blood pressure levels between the nonsmokers and smokers might partly result from different interactions of several SNPs and cigarette smoking.

  10. Investigating Consumer Purchase Behavior in Related Technology Product Categories

    OpenAIRE

    S. Sriram; Chintagunta, Pradeep K.; Agarwal, Manoj K.

    2010-01-01

    We present a framework of durable goods purchasing behavior in related technology product categories that incorporates the following aspects unique to technology product purchases. First, it accounts for consumers' anticipation of declining prices (or increasing quality) over time. Second, the durable nature of technology products implies that even if two categories are related as complements, consumers may stagger their purchases over several periods. Third, the forward-looking consumer deci...

  11. Markers of collagen synthesis is related to blood pressure and vascular hypertrophy: a LIFE substudy

    DEFF Research Database (Denmark)

    Olsen, M H; Christensen, M K; Wachtell, K; Tuxen, Christian; Fossum, E; Bang, L E; Wiinberg, N; Devereux, R B; Kjeldsen, S E; Hildebrandt, Per; Dige-Petersen, H; Rokkedal, J; Ibsen, H

    2005-01-01

    Cardiac fibrosis and high levels of circulating collagen markers has been associated with left ventricular (LV) hypertrophy. However, the relationship to vascular hypertrophy and blood pressure (BP) load is unclear. In 204 patients with essential hypertension and electrocardiographic LV hypertrophy...... losartan- or an atenolol-based regimen. Furthermore, we measured intima-media thickness of the common carotid arteries (IMT), minimal forearm vascular resistance (MFVR) by plethysmography and ambulatory 24-h BP in around half of the patients. At baseline, PICP/ICTP was positively related to IMT (r=0.24, P...

  12. Blood pressure, atherosclerosis, and the incidence of age-related maculopathy: the Rotterdam Study

    OpenAIRE

    Leeuwen, Redmer; Ikram, Kamran; Vingerling, Hans; Witteman, Jacqueline; Jong, Paulus; Hofman, Albert

    2003-01-01

    textabstractPURPOSE: To determine whether blood pressure and subclinical atherosclerosis are associated with incident age-related maculopathy (ARM). METHODS: The study was performed within the Rotterdam Study, a population-based, prospective cohort study in Rotterdam, The Netherlands. A total of 4822 subjects who at baseline were aged 55 years more, were free of ARM, and participated in at least one of two follow-up examinations after a mean of 2 and 6.5 years, were included in the study. At ...

  13. Artificial blood

    Directory of Open Access Journals (Sweden)

    Sarkar Suman

    2008-01-01

    Full Text Available Artificial blood is a product made to act as a substitute for red blood cells. While true blood serves many different functions, artificial blood is designed for the sole purpose of transporting oxygen and carbon dioxide throughout the body. Depending on the type of artificial blood, it can be produced in different ways using synthetic production, chemical isolation, or recombinant biochemical technology. Development of the first blood substitutes dates back to the early 1600s, and the search for the ideal blood substitute continues. Various manufacturers have products in clinical trials; however, no truly safe and effective artificial blood product is currently marketed. It is anticipated that when an artificial blood product is available, it will have annual sales of over $7.6 billion in the United States alone.

  14. Perspectives and Problems related to the Shale Gas Production

    Directory of Open Access Journals (Sweden)

    D. P. Gerish

    2014-12-01

    Full Text Available This article describes the main aspects of shale gas production, its origin and properties. The particular issues related to the shale gas production, as well as the public and official attitude to the perspectives of its usage in Russian Federation are analyzed. The general problems of the shale gas exploration and production in the Russian Federation are discussed. The current and prognostic rates of shale gas production in USA are shown.

  15. Perspectives and Problems related to the Shale Gas Production

    OpenAIRE

    D. P. Gerish; O. E. Kochneva

    2014-01-01

    This article describes the main aspects of shale gas production, its origin and properties. The particular issues related to the shale gas production, as well as the public and official attitude to the perspectives of its usage in Russian Federation are analyzed. The general problems of the shale gas exploration and production in the Russian Federation are discussed. The current and prognostic rates of shale gas production in USA are shown.

  16. Dry period cooling ameliorates physiological variables and blood acid base balance, improving milk production in murrah buffaloes

    Science.gov (United States)

    Aarif, Ovais; Aggarwal, Anjali

    2016-03-01

    This study aimed to evaluate the impact of evaporative cooling during late gestation on physiological responses, blood gas and acid base balance and subsequent milk production of Murrah buffaloes. To investigate this study sixteen healthy pregnant dry Murrah buffaloes (second to fourth parity) at sixty days prepartum were selected in the months of May to June and divided into two groups of eight animals each. One group of buffaloes (Cooled/CL) was managed under fan and mist cooling system during dry period. Group second buffaloes (Noncooled/NCL) remained as control without provision of cooling during dry period. The physiological responses viz. Rectal temperature (RT), Respiratory rate (RR) and Pulse rate were significantly ( P group 2, with the provision of cooling. Skin surface temperature at thorax was significantly lower in cooled group relative to noncooled group. Blood pH and pO2 were significantly ( P group as compared to the cooled group. pCO2, TCO2, HCO3, SBC, base excess in extracellular fluid (BEecf), base excess in blood (BEb), PCV and Hb were significantly ( P group as compared to noncooled group. DMI was significantly ( P group of Murrah buffaloes.

  17. Thermodynamic Product Relations for Generalized Regular Black Hole

    CERN Document Server

    Pradhan, Parthapratim

    2016-01-01

    We derive thermodynamic product relations for four-parametric regular black hole(BH) solutions of the Einstein equations coupled with a non-linear electrodynamics source. The four parameters can be described by the mass ($m$), charge ($q$), dipole moment ($\\alpha$) and quadrupole moment ($\\beta$) respectively. We study its complete thermodynamics. We compute different thermodynamic products i.e. area product, BH temperature product, specific heat product and Komar energy product respectively. Furthermore, we show that some complicated function of horizon areas that is indeed \\emph{mass-independent} and could turn out to be \\emph{universal}.

  18. Blood Metal Concentrations of Manganese, Lead, and Cadmium in Relation to Serum Ferritin Levels in Ohio Residents

    Science.gov (United States)

    The objectives of this study were to assess fcrritin-specific profiles of blood metal concentrations such as manganese, lead, and cadmium and to evaluate whether ferritin may affect the behavior of the blood metals in relation to menstruation, menopause, or sex in Ohio residents....

  19. Perioperative use of allogenic blood components in live-related donor orthotopic liver transplantation: A cross sectional study

    Directory of Open Access Journals (Sweden)

    Prashant Pandey

    2013-01-01

    Full Text Available Background: In spite of many improvements that have reduced the blood component requirements, substantial numbers of transfusions are still needed in liver transplantation. Aims: The objective of the present study was to analyze the perioperative usage of allogenic blood components and predict the preoperative factors as predictors of red cell transfusion in live-related donor liver transplant recipients. Materials and Methods: The retrospective data on utilization of allogenic blood components were analyzed for a total of 150 liver transplant procedures. The data on utilization of blood components during surgery and till 48 hours of ICU stay was collected from the blood bank record and hospital information system (HIS. Results: Red cell concentrate was commonest blood component used in liver transplant recipient and most of the transfusion took place during surgery. During intraoperative period 92.7% (N = 139 of the cases utilized red cell components with the median number of five whereas in postoperative period only 38% (N = 57 of patients received blood with the median number of one. This study demonstrates that the preoperative hemoglobin and platelet count are the predictors of utilization of red cell concentrates during surgery. There were a total of 11 (7.3% recipients who didn′t receive allogeneic blood transfusion in any form.Utilization of blood components was negligible among organ donors. Conclusion: Our study demonstrates the pattern and predictors of usage of allogeneic blood components in liver transplant recipients at a tertiary healthcare center in India.

  20. Age-related alteration in the composition of immunocompetent blood cells in atomic bomb survivors

    International Nuclear Information System (INIS)

    1328 survivors of Hiroshima were studied for alterations in the number of blood lymphocytes belonging to T-cell subpopulations, CD19 antigen-positive B cells and Leu 7 and CD16 antigen-positive lymphocytes. With increasing age, significant decreasing trends in the numbers of some lymphocytes in T-cell subpopulations and of B-cells were seen. The number of blood lymphocytes positive for CD5 antigen was significantly lower in those exposed to radiation (> 1Gy) in the older age group (more than 30 years at the time of bombing) and a similar tendency for decreases in the numbers of CD4, CD8, and CD19 antigen-positive cells was observed, but differences were not significant. The results suggest aging of the T-cell related immune system is accelerated in the irradiated people of advanced age, explained by the age-related decrease in thymic function in those subjects. The number of Leu 7 or CD19 antigen-positive cells was found to be increased significantly in the older age group compared to the younger, although there was little dose dependence. (U.K.)

  1. Pharmaceutical protein production by yeast: towards production of human blood proteins by microbial fermentation

    OpenAIRE

    Martínez, José L.; Liu, Lifang; Petranovic, Dina; Nielsen, Jens

    2012-01-01

    Since the approval of recombinant insulin from Escherichia coli for its clinical use in the early 1980s, the amount of recombinant pharmaceutical proteins obtained by microbial fermentations has significantly increased. The recent advances in genomics together with high throughput analysis techniques (the so-called - omics approaches) and integrative approaches (systems biology) allow the development of novel microbial cell factories as valuable platforms for large scale production of therape...

  2. Comparative usefulness of inflammatory markers to indicate bacterial infection-analyzed according to blood culture results and related clinical factors.

    Science.gov (United States)

    Nishikawa, Hirokazu; Shirano, Michinori; Kasamatsu, Yu; Morimura, Ayumi; Iida, Ko; Kishi, Tomomi; Goto, Tetsushi; Okamoto, Saki; Ehara, Eiji

    2016-01-01

    To assess relationships of inflammatory markers and 2 related clinical factors with blood culture results, we retrospectively investigated inpatients' blood culture and blood chemistry findings that were recorded from January to December 2014 using electronic medical records and analyzed the data of 852 subjects (426 culture-positive and 426 culture-negative). Results suggested that the risk of positive blood culture statistically increased as inflammatory marker levels and the number of related factors increased. Concerning the effectiveness of inflammatory markers, when the outcome definition was also changed for C-reactive protein (CRP), the odds ratio had a similar value, whereas when the outcome definition of blood culture positivity was used for procalcitonin (PCT), the greatest effectiveness of that was detected. Therefore, the current results suggest that PCT is more useful than CRP as an auxiliary indication of bacterial infection. PMID:26525643

  3. Age-related changes in regional cerebral blood flow and brain volume in healthy subjects

    International Nuclear Information System (INIS)

    Using the xenon-133 inhalation method, we studied the age-related decline in regional cerebral blood flow, calculated as the initial slope index (ISI), in neurologically normal subjects without any risk factors for cerebral arteriosclerosis (154 men and 123 women), ranging in age from 19 to 88 years. The decline in the ISI was rapid in younger age groups and gradual in older age groups. The ISI was higher in women than in men older than 40 years. Using computed tomography, we studied the age-related decline in brain volume index (BVI; 100% X brain volume/cranial cavity volume) in neurologically normal subjects without any risk factors for cerebral arteriosclerosis (92 men and 49 women), ranging in age from 37 to 86 years. The decline in the BVI was gradual in younger age groups and rapid in older age groups. The BVI was higher in women than in men older than 60 years

  4. INFLUENCE OF ALPHA-1-ACID GLYCOPROTEIN UPON PRODUCTION OF CYTOKINES BY PERIPHERAL BLOOD MONONUCLEARS

    Directory of Open Access Journals (Sweden)

    М. V. Osikov

    2014-07-01

    Full Text Available Abstract. Alpha-1-acid glycoprotein (orosomucoid is a multifunctional acute phase reactant belonging to the family of lipocalines from plasma alpha-2 globulin fraction. In present study, we investigated dosedependent effects of orosomucoid upon secretion of IL-1â, IL-2, IL-3, IL-4 by mononuclear cells from venous blood of healthy volunteers. Mononuclear cells were separated by means of gradient centrifugation, followed by incubation for 24 hours with 250, 500, or 1000 mcg of orosomucoid per ml RPMI-1640 medium (resp., low, medium and high dose. The levels of cytokine production were assayed by ELISA technique. Orosomucoid-induced secretion of IL-1â and IL-4 was increased, whereas IL-3 secretion was inhibited. IL-2 production was suppressed at low doses of orosomucoid, and stimulated at medium and high doses. The effect of alpha-1-acid glycoprotein upon production of IL-2, IL-3 and IL-4 was dose-dependent. Hence, these data indicate that orosomucoid is capable of modifying IL-1â, IL-2, IL-3, and IL-4 secretion by blood mononuclear cells.

  5. Circulating thrombopoietin levels in normal healthy blood donors and in aplastic anemia patients in relation to disease severity

    Directory of Open Access Journals (Sweden)

    Abhay Singh

    2015-01-01

    Full Text Available Background: Thrombopoietin (TPO is the key hematopoietic growth factor regulating the production of platelets from bone marrow megakaryocytes and maintaining platelet hemostasis. This study was done to find any relationship between the levels of thrombopoietin and the severity of disease in patients with aplastic anemia. Materials and Methods: Serum samples were collected from 52 patients with a confirmed diagnosis of aplastic anemia and 45 normal healthy blood donors of both sexes over a period of 2 years, and TPO was estimated by using commercially available TPO-specific-enzyme-linked immunosorbent assay. Results: The median TPO level of 1190 pg/ml (range 625-7651 pg/ml in aplastic anemia patients was significantly higher than the median TPO level of 121.1 pg/ml (81.25-237.7 pg/ml in normal healthy blood donors (P = 0.000. No significant difference was observed in TPO levels of male and female patients (P = 0.453. The median TPO concentrations observed in very severe aplastic anemia, severe aplastic anemia, and nonsevere aplastic anemia were 2765 pg/ml (range 625-6451 pg/ml, 1190 pg/ml (range 672.1-7651 pg/ml, and 1111.5 pg/ml (range 761.1-2289.2 pg/ml, respectively. TPO in patients of very severe aplastic anemia was significantly higher than patients of nonsevere aplastic anemia (P = 0.043, with no significant relation among rest of the groups. Discussion: TPO levels in aplastic anemia patients were significantly higher than in healthy blood donors; however, in aplastic anemia patients TPO levels were significantly higher only in patients with very severe disease.

  6. Influence of some probiotics on blood components, productive and reproductive performance of japanese quails

    International Nuclear Information System (INIS)

    The current study was conducted to evaluate the effect of adding some probiotics into diets of Japanese quails on productive and reproductive performance, blood constituents, immune response and intestinal wall thickness. A total number of 500 one day old, unsexed Japanese quail chicks were used. Five experimental groups were assigned in the present study, where the 1st group (G1) was provided with protexin at 5 g/litre (5 days weekly). The 2nd group (G2) contained medi stress of 0.75 g/litre (5 days weekly), the 3rd group (G3) was fed a basal diet contained bioaction at 5g/kg, the 4th group (G4) involved a basal diet with active dried yeast (Saccharomyces cerevisiae) at 0.1 g/kg daily and group 5 were fed the basal diet without probiotics supplementation (control). The results showed that the average body weight, egg production and egg weight were significantly increased due to adding probiotics and 0.1g/kg yeast into diets. Feed conversion ratio and mortality were improved significantly and the most improvement was recorded at 1.0% yeast and protexin. Similar improvement was recorded for hatch ability and hatching weight. There were reductions in intestinal wall thickness due to adding 0.1 g/kg yeast or protexin. The values of HI titer against sheep RBCs were increased significantly as compared to the control, which may lead to an enhancement of immune response of Japanese quails. Blood protein, globulin and FSH hormone were increased significantly. In conclusion, adding some probiotics into diets of Japanese quails improved productive, reproductive performance, blood constituents, immune response and intestinal wall thickness

  7. Scotblood 2015: Improving and delivering blood products, novel cellular therapies, and celebrating patients and donor engagement within transfusion services.

    Science.gov (United States)

    Colligan, David; McGowan, Neil; Seghatchian, Jerard

    2016-08-01

    Blood Transfusion Services are striving to continually improve the efficacy and quality of their blood products whilst also simultaneously diversifying into novel cellular products. For this to be successful the relationships between the various arms of the organisation must be strong and interlinked. As new technologies impact on the products that blood transfusion services supply it should be noted that the interaction between the service and its donor base is also affected by advancing technologies. Social media has fundamentally altered the way in which the public can access information and news, as such blood services must engage and interact appropriately with these new forms of media. As a reflection of these challenges the Scotblood 2015 programme was focussed on service and product improvement, donor engagement and people centred transfusion. This commentary comprises summaries of the presentations, based in part on the abstracts provided by the speakers. PMID:27524267

  8. Outcome-Driven Thresholds for Home Blood Pressure Measurement International Database of HOme blood pressure in relation to Cardiovascular Outcome

    OpenAIRE

    Niiranen, T. J.; Asayama, K.; Thijs, L; Johansson, J K; Ohkubo, T.; Kikuya, M; Boggia, J.; Hozawa, A.; Sandoya, E.; Stergiou, G. S.; Tsuji, I; Jula, A. M.; Imai, Y.; Staessen, J. A.

    2013-01-01

    The lack of outcome-driven operational thresholds limits the clinical application of home blood pressure (BP) measurement. Our objective was to determine an outcome-driven reference frame for home BP measurement. We measured home and clinic BP in 6470 participants (mean age, 59.3 years; 56.9% women; 22.4% on antihypertensive treatment) recruited in Ohasama, Japan (n=2520); Montevideo, Uruguay (n=399); Tsurugaya, Japan (n=811); Didima, Greece (n=665); and nationwide in Finland (n=2075). In mul...

  9. Blood Pressure Standards for Shiraz (Southern Iran School Children in Relation to Height

    Directory of Open Access Journals (Sweden)

    Seyyed Mohammad-Taghi Ayatollahi

    2012-06-01

    Full Text Available Objective: This study aims at providing local reference values for blood pressure by height and determining distribution pattern of systolic and diastolic blood pressure in 6.5-11.5 elementary school children for the first time in Shiraz (Southern Iran.Methods: Height, systolic blood pressure (SBP and diastolic blood pressure (DBP were measured with standard methods in 2270 healthy school children (1174 boys, 1096 girls who were selected by multi-stage random sampling in 2003-2004 academic years. We produced blood pressure percentiles by height percentiles using Healy-Rasbash-Yang method.Findings: The blood pressure (both systolic and diastolic tends to increase with age, but after adjusting the measurements for height, no significant correlation was found between either systolic blood pressure or diastolic blood pressure and age (r=-0.03 and P=0.15 for systolic blood pressure, r=-0.005 and P=0.8 for diastolic blood pressure. Then systolic and diastolic blood pressure percentile values by age and height percentiles, and blood pressure smoothed centiles by height in 6.5-11.5 years school children were derived.Conclusion: Due to genetic, cultural and environmental differences among populations, it is suggested to use local blood pressure standards in Iran. We produced blood pressure percentiles by height instead of age because it seems that it would lead to better evaluation for real hypertensive diagnosis.

  10. Changes in Blood Parameters and the Expression of Coagulation-Related Genes in Lactating Sprague–Dawley Rats

    OpenAIRE

    Urasoko, Yoshinaka; He, Xi Jun; Masao, Takano; KINOSHITA, YUICHI; Edamoto, Hiroshi; Hatayama, Kazuhisa; Asano, Yuzo; Tamura, Kazutoshi; Mochizuki, Masahiro

    2012-01-01

    This study measured blood parameters, particularly those related to coagulation, and alterations in the expression levels of blood-coagulation–related genes in lactating Sprague–Dawley rats. The day of delivery was designated as lactation day 0 (LD 0). On the day after delivery (LD 1), prothrombin time and overall activity of vitamin-K–dependent coagulation factors were decreased, whereas fibrinogen contents, platelet counts and antithrombin III concentrations were increased as compared with ...

  11. The Deployment of Product-Related Environmental Legislation into Product Requirements

    DEFF Research Database (Denmark)

    Pigosso, Daniela Cristina Antelmi; Ferraz, Mariana; Teixeira, Cláudia; Rozenfeld, Henrique

    2016-01-01

    (PDP). Nevertheless, the deployment of product-related environmental legislation into product requirements is still a challenge. This study followed an inductive approach to propose a guideline to support the identification, analysis and deployment of product requirements based on product......Environmental legislation is increasingly changing its focus from manufacturing-oriented to product-oriented instruments. Compliance with product-related environmental legislation is achieved by the incorporation of environmental requirements into the early phases of the product development process......-related environmental legislation. The guideline is composed of nine steps, clustered into three groups according to their main objective: (A) identification of environmental product-related legislation; (B) identification of legislative topics to be considered for the deployment of requirements; and (C) creation and...

  12. An economic evaluation of plasma production via erythroplasmapheresis and whole blood collection.

    Science.gov (United States)

    Connelly, Luke B; Pink, Anne

    2002-10-01

    This paper presents cost-effectiveness analyses (CEAs) of plasma collection via two alternative methods: whole blood collection (WBC) and erythroplasmapheresis collection (EPC). The objective of the study is to provide an answer to the question 'What is the least-cost method of plasma production'. This question is answered, both from the viewpoint of the blood collection agency (using financial CEA) and from that of 'society' as a whole (using economic CEA). We employ detailed financial data and economic survey data for collections made by a blood collection agency and to WBC and EPC donors in Brisbane, Australia. The results indicate that, despite the superior yield provided by EPC, WBC is actually more cost-effective. This result is robust to thorough sensitivity analysis and arises regardless of whether an economic or financial perspective is taken. We conclude that, ceteris paribus, the cost of recruiting new plasma donors would need to be quite substantial for marginal investments in EPC to be considered cost-effective. PMID:12350045

  13. Peripheral Blood Leukocyte Production of BDNF following Mitogen Stimulation in Early Onset and Regressive Autism

    Directory of Open Access Journals (Sweden)

    Amanda Enstrom

    2008-01-01

    Full Text Available Brain-derived neurotrophic factor (BDNF is critical for neuronal differentiation and synaptic development. BDNF is also implicated in the development of psychological disorders including depression, bipolar disorder and schizophrenia. Previously, elevated BDNF levels were observed in neonatal blood samples from infants who were later diagnosed with autism when compared with children who developed normally, suggesting that BDNF may be involved in the development of autism. BDNF is produced by activated brain microglial cells, a cellular phenotype that shares several features with peripheral macrophages, suggesting an important role for the immune system in BDNF production. We hypothesized that under mitogenic stimulation, peripheral blood mononuclear cells obtained from children with autism may have altered BDNF production compared with age-matched typically developing control subjects. In addition, we examined the differences between the production of BDNF in classic/early-onset autism and children who had a regressive form of autism. We show here that plasma levels of BDNF levels are increased in children with autism, especially in early onset autism subjects. Furthermore, under mitogenic stimulation with PHA and LPS, BDNF production is significantly increased in children with autism compared with typically developing subjects. However, stimulation with tetanus toxoid results in a decreased response in children with autism. This data suggest that immune cell-derived production of BDNF could be an important source for the increased BDNF that is detected in some subjects with autism. As a neurotrophic factor produced by immune cells, BDNF could help elucidate the role of the immune system in neurodevelopment and neuronal maintenance, which may be dysregulated in autism.

  14. ABO Blood Group. Related Investigations and Their Association with Defined Pathologies

    Directory of Open Access Journals (Sweden)

    Ursula Jesch

    2007-01-01

    Carriers of blood group O suffered from ulcus ventriculi and gastritis (X21 = 78.629, p <0.001, colitis ulcerosa and duodenitis (X21 = 5.846, p < 0.016, whereas male patients carrying blood group A tended to contract different types of tumours. In patients with intestinal tumours, females with blood group A were more likely to develop the pathology, whereas in males, the blood group O dominated. The development of cholelithiasis was found, above all, in patients with blood group O, which differed from other research where a correlation between this pathology and blood group A was found.

  15. Mining Intention-Related Products on Online Q & A Community

    Institute of Scientific and Technical Information of China (English)

    段俊文; 陈毅恒; 刘挺; 丁效

    2015-01-01

    User generated content on social media has attracted much attention from service/product providers, as it contains plenty of potential commercial opportunities. However, previous work mainly focuses on user consumption intention (CI) identification, and little effort has been spent to mine intention-related products. In this paper, focusing on the Baby &Child Care domain, we propose a novel approach to mine intention-related products on online question and answer (Q&A) community. Making use of the question-answering pairs as data source, we first automatically extract candidate products based on dependency parser. And then by means of the collocation extraction model, we identify the real intention-related products from the candidate set. The experimental results on our carefully constructed evaluation dataset show that our approach achieves better performance than two natural baseline methods.

  16. The influences of SE infection on layers’ production performance, egg quality and blood biochemical indicators

    Science.gov (United States)

    2014-01-01

    Background Salmonella enterica serovar Enteritidis (SE), as a major cause of foodborn illness, infects humans mainly through the egg. However, the symptom of laying hens usually is not typical and hard to diagnosis. In the present study, it is studied that the influences of SE infection on layers’ performance, egg quality and blood biochemical indicators. It will help us to improve the strategy to control SE infection in commercial layers. One hundred layers at 20 wk of age were divided into 2 groups, 60 hens for experiment and others for control. The experiment group was fed with the dosage of 108 CFU SE per hen. The specific PCR was used to detect the deposition of SE. On the 8 d after SE infection, 10 hens from the control group and 30 hens from the experimental group were slaughtered to detect the SE colonization. The production performance, egg quality and blood biochemical indices were also analyzed. Results The results showed that the colonization rate of SE was highest in caecum contents (55.17%) and lowest in vagina (17.24%). For the eggs the detection rate of SE was highest on the eggshell (80.00%) and lowest in yolk (18.81%). SE infection had no significant influence on production performance and egg qualities (P > 0.05). The difference of laying rate between the experimental and control groups was less than 0.30%, and both were approximately equal to 82.00%. The blood analysis showed that the aspartic aminotransferase (AST) and alanine aminotransferase (ALT) of experimental group was significantly higher than those of control group (P < 0.05). For experimental and control groups AST values were 236.22 U/l and 211.84 U/l respectively, and ALT values were 32.19 U/l and 24.55 U/l. All of coefficients were less than 20%. The colonization of SE in organs increases the enzyme activities of AST and ALT in blood. Conclusions SE in feed could invade the oviduct and infect the forming eggs. It significantly increased the concentration of ALT and AST in blood

  17. The influences of SE infection on layers’ production performance, egg quality and blood biochemical indicators

    Institute of Scientific and Technical Information of China (English)

    Shijie Fan; Jiangxia Zheng; Zhongyi Duan; Ning Yang; Guiyun Xu

    2014-01-01

    Background:Salmonella enterica serovar Enteritidis (SE), as a major cause of foodborn illness, infects humans mainly through the egg. However, the symptom of laying hens usually is not typical and hard to diagnosis. In the present study, it is studied that the influences of SE infection on layers’ performance, egg quality and blood biochemical indicators. It will help us to improve the strategy to control SE infection in commercial layers. One hundred layers at 20 wk of age were divided into 2 groups, 60 hens for experiment and others for control. The experiment group was fed with the dosage of 108 CFU SE per hen. The specific PCR was used to detect the deposition of SE. On the 8 d after SE infection, 10 hens from the control group and 30 hens from the experimental group were slaughtered to detect the SE colonization. The production performance, egg quality and blood biochemical indices were also analyzed. Results:The results showed that the colonization rate of SE was highest in caecum contents (55.17%) and lowest in vagina (17.24%). For the eggs the detection rate of SE was highest on the eggshell (80.00%) and lowest in yolk (18.81%). SE infection had no significant influence on production performance and egg qualities (P>0.05). The difference of laying rate between the experimental and control groups was less than 0.30%, and both were approximately equal to 82.00%. The blood analysis showed that the aspartic aminotransferase (AST) and alanine aminotransferase (ALT) of experimental group was significantly higher than those of control group (P<0.05). For experimental and control groups AST values were 236.22 U/l and 211.84 U/l respectively, and ALT values were 32.19 U/l and 24.55 U/l. All of coefficients were less than 20%. The colonization of SE in organs increases the enzyme activities of AST and ALT in blood. Conclusions:SE in feed could invade the oviduct and infect the forming eggs. It significantly increased the concentration of ALT and AST in blood

  18. Review of xenon-133 production and related problems

    International Nuclear Information System (INIS)

    A literature survey is given on the production methods of fission xenon-133 and related problems, such as purification, metrological and dosimetric aspects, preparation of isotopic solutions, recycling, etc. 127 references are included. (Author) 127 refs

  19. Social relations within organic food production and consumption

    OpenAIRE

    Galbačová, Ivana

    2011-01-01

    This thesis examines the social relations of production and consumption of organic food in the Czech Republic. Organic farming and organic products are new phenomena in nutrition in the countries of the European Union and in the Czech Republic. The current population through sustainable development is looking more and more sources of income that would not only benefit the health and meet the criteria of a healthy lifestyle, but their production should be as environmentally friendly as poss...

  20. Rilmenidine prevents blood pressure increase in rats with compromised nitric oxide production

    Institute of Scientific and Technical Information of China (English)

    Mária GEROV(A); Jozef T(O)R(O)K; Ol'ga PECH(A)(O)OV(A); Jana MATU(S)KOV(A)

    2004-01-01

    AIM: To search tools of high blood pressure in the model of nitric oxide (NO)-defective hypertension, and the study focused on the effect of rilmenidine, agonist of imidazoline receptors, which was suggested to modulate central sympathetic outflow. METHODS: Three experimental groups, each consisting of 7 rats, were used: (Ⅰ) rats with inhibition of NO synthase (NOS) by NG-nitro-L-arginine methyl ester (L-NAME) 40 mg.kg-1.d-1 for 4 weeks in drinking water, (Ⅱ) rats with inhibited NOS as in group Ⅰ, plus agonist of imidazoline receptors rilmenidine 3mg.kg-1·d-1 for 4 weeks by gavage, and (Ⅲ) control rats. Systolic blood pressure was measured weekly noninvasively.At the end of experiment aortic ring isometric tension was followed, NOS expression (aorta, left ventricle), and NOS activity (left ventricle and brain) were determined. RESULTS: In the group Ⅰ systolic blood pressure increased significantly, aortic ring relaxation to acetylcholine was significantly attenuated. Rilmenidine administered simultaneously with L-NAME (group Ⅱ) prevented the increase of blood pressure which did not differ significantly from control values; aortic ring relaxation to acetylcholine did not differ from control. No change in NOS expression (aorta and left ventricle) was found in groups Ⅰ and Ⅱ. Significant decline in NOS activity (left ventricle and brain) was found in groups Ⅰ and ⅡⅡ. CONCLUSION: Rilmenidine has a remarkable role in NO-defective hypertension,possibly by inhibiting central sympathetic outflow and by affecting receptors in vascular smooth muscle also. The prime cause of hypertension in this experimental model - the compromised production of NO due to inhibition of NOS - was not affected by rilmenidine.

  1. Directory of PCB-related product and service companies

    International Nuclear Information System (INIS)

    The directory lists 28 companies from coast to coast whose principal business activity involves PCB-related products and services. Entries are in alphabetical order. Basic information provided for each of the companies includes: name, address, telephone and fax number, and a brief description of the special product or service provided

  2. Chronic whiplash symptoms are related to altered regional cerebral blood flow in the resting state.

    Science.gov (United States)

    Linnman, Clas; Appel, Lieuwe; Söderlund, Anne; Frans, Orjan; Engler, Henry; Furmark, Tomas; Gordh, Torsten; Långström, Bengt; Fredrikson, Mats

    2009-01-01

    The neural pathogenic mechanisms involved in mediating chronic pain and whiplash associated disorders (WAD) after rear impact car collisions are largely unknown. This study's first objective was to compare resting state regional cerebral blood flow (rCBF) by means of positron emission tomography with (15)O labelled water in 21 WAD patients with 18 healthy, pain-free controls. A second objective was to investigate the relations between brain areas with altered rCBF to pain experience, somatic symptoms, posttraumatic stress symptoms and personality traits in the patient group. Patients had heightened resting rCBF bilaterally in the posterior parahippocampal and the posterior cingulate gyri, in the right thalamus and the right medial prefrontal gyrus as well as lowered tempero-occipital blood flow compared with healthy controls. The altered rCBF in the patient group was correlated to neck disability ratings. We thus suggest an involvement of the posterior cingulate, parahippocampal and medial prefrontal gyri in WAD and speculate that alterations in the resting state are linked to an increased self-relevant evaluation of pain and stress. PMID:18486506

  3. A Review of Health-Related Workplace Productivity Loss Instruments

    OpenAIRE

    Lofland, Jennifer H.; Laura Pizzi; Frick, Kevin D.

    2004-01-01

    The objective of this review was to identify health-related workplace productivity loss survey instruments, with particular emphasis on those that capture a metric suitable for direct translation into a monetary figure. A literature search using Medline, HealthSTAR, PsycINFO and Econlit databases between 1966 and 2002, and a telephone-administered survey of business leaders and researchers, were conducted to identify health-related workplace productivity measurement survey instruments. This r...

  4. Kinetic study of cytokines production by human peripheral blood mononuclear cells in response to Brucella DNA.

    Science.gov (United States)

    Lashkarbolouki, Taghi; Ardestani, Sussan K; Kariminia, Amina; Ziaee, Abed-Ali; Torkabadi, Ebrahim; Ebrahimi, Mohammad

    2008-01-01

    In spite of reports on cytokines induction by the Brucella DNA in murine model, there is no comparison between pathogenic and appropriate vaccine strains in human. We investigated the cytokines profile of human peripheral blood mononuclear cells (PBMCs) induced by DNA extracted from pathogenic isolates of Brucella melitensis and B. abortus as well as Rev1 and S19; the appropriate vaccine strains. It was observed that despite differential induction of Interleukin(IL)-12 and IL-10 production, identical IL-12/IL-10 concentration ratio was obtained by all Brucella strains DNAs that was 2 after 24 h and 4 after 5 days of incubation. In addition, IL-2 and Interferon(IFN)-gamma production were profoundly increased compared to the medium at day 3 and 5 respectively but IFN-alpha was not induced. Therefore, Brucella strains DNAs are Th1 inducing component with similar pattern in human PBMCs. PMID:17008080

  5. Relative value of diverse brain MRI and blood-based biomarkers for predicting cognitive decline in the elderly

    Science.gov (United States)

    Madsen, Sarah K.; Ver Steeg, Greg; Daianu, Madelaine; Mezher, Adam; Jahanshad, Neda; Nir, Talia M.; Hua, Xue; Gutman, Boris A.; Galstyan, Aram; Thompson, Paul M.

    2016-03-01

    Cognitive decline accompanies many debilitating illnesses, including Alzheimer's disease (AD). In old age, brain tissue loss also occurs along with cognitive decline. Although blood tests are easier to perform than brain MRI, few studies compare brain scans to standard blood tests to see which kinds of information best predict future decline. In 504 older adults from the Alzheimer's Disease Neuroimaging Initiative (ADNI), we first used linear regression to assess the relative value of different types of data to predict cognitive decline, including 196 blood panel biomarkers, 249 MRI biomarkers obtained from the FreeSurfer software, demographics, and the AD-risk gene APOE. A subset of MRI biomarkers was the strongest predictor. There was no specific blood marker that increased predictive accuracy on its own, we found that a novel unsupervised learning method, CorEx, captured weak correlations among blood markers, and the resulting clusters offered unique predictive power.

  6. The relation of consciousness level and cerebral blood flow in glioma and meningioma

    International Nuclear Information System (INIS)

    The relation between the consciousness level and cerebral blood flow (CBF) was analyzed in 17 cases of glioma and 6 cases of meningioma. CBF was in normal range in the alert groups of patients with glioma and meningioma. By contrast, CBF decreased generally not only in the affected side, but also in the non-affected side in the drowsy patient group with glioma. Widespread infiltration of glioma was seen in 4 cases of 6 drowsy cases. CBF increased after the partial resection of the tumor in 2 cases of glioma. Thus the disturbance of consciousness level was attributable to diffuse hypofunction accompanying the diffuse infiltration of glioma and the decrease of cerebral perfusion pressure caused by the increased intracranial pressure. (author)

  7. The association between Neovascular Age-related Macular Degeneration and Regulatory T cells in peripheral blood

    DEFF Research Database (Denmark)

    Madelung, Christopher Fugl; Falk, Mads; Sørensen, Torben Lykke

    2015-01-01

    PURPOSE: To investigate regulatory T cells (Tregs) and subsets of the Treg population in patients with neovascular age-related macular degeneration (AMD). PATIENTS AND METHODS: Twenty-one neovascular AMD cases and 12 age-matched controls without retinal pathology were selected. Patients were...... recruited from our outpatient retinal clinic. Control individuals were typically spouses. The diagnosis of neovascular AMD was confirmed using fluorescein and indocyaningreen angiography. Fresh venous blood was analyzed by flow cytometry using fluorochrome-conjugated antibodies to the Treg surface antigens...... CD4, CD25, CD127, CD45RA, and CD31. Main outcome measures were the percentage of CD25(high)CD127(low) Tregs, the percentage of CD45RA(+) naïve Tregs, and the percentage of CD31(+) recent thymic emigrant Tregs. RESULTS: Comparing patients with neovascular AMD to controls, no significant differences...

  8. First two cases of living related liver transplantation with complicated anatomy of blood vessels in Beijing

    Institute of Scientific and Technical Information of China (English)

    Wen-Han Wu; Yuan-Lian Wan; Long Lee; Yin-Mo Yang; Yan-Ting Huang; Chao-Long Chen; Sheung-Tat Fan

    2004-01-01

    AIM: Living related liver transplantation (LRLT) has been developed in response to the paediatric organ donor shortage.Though it has been succeeded in many centers worldwide,the safety of the donor is still a major concern, especially in donors with anatomy variation. We succeeded in performing the first two cases of living related liver transplantation with complicated anatomy of blood vessels as a way to overcome cadaveric organ shortage in Beijing.METHODS: Two patients, with congenital liver fibrosis and congenital biliary atresia were performed with living donor liver transplantation in our hospital and then followed up from November 12 to December 13, 2001. The two living donors, mother and father, were healthy aged 34 and 35years. One right lobe (segment Ⅴ, Ⅵ, Ⅶ, Ⅷ) and one left lateral lobe (segment Ⅱ and Ⅲ) were used. The grafts weighed 394 g and 300 g. The ratio of graft weight to the standard liver volume (SLV) of donors was 68% and 27%.The graft weight to recipient body weight ratio was 3.2%and 4.4%. The graft weight to recipient estimated standard liver mass (ESLM) ratio was 63% and 85%. The two donors had complicated blood vessel variation.RESULTS: Two patients undergone living donor liver transplantation had good results. Abnormal liver function with high bilirubin level appeared in a few days after operation, but liver function returned to normal one month after operation with bilirubin level almost decreased to near normal. No bleeding, thrombosis, infection and bile leakage occurred. One had an acute rejection and recovered.The two donors recovered in two weeks. One had slight fever because of a little collection in abdomen and recovered after paracentesis and drainage.CONCLUSION: Living donor liver transplantation has been proved to be a good way that offers a unique opportunity of getting a timely liver graft as a response to shortage of pediatric donors, though it could be a technically difficult operation if there is anatomical

  9. Illness related wage and productivity losses: Valuing 'presenteeism'.

    Science.gov (United States)

    Zhang, Wei; Sun, Huiying; Woodcock, Simon; Anis, Aslam

    2015-12-01

    One source of productivity loss due to illness is the reduced "quantity" or "quality" of labor input while working, often referred to as presenteeism. Illness-related presenteeism has been found to be potentially more costly than absenteeism. To value presenteeism, existing methods use wages as a proxy for marginal productivity at the firm level. However, wage may not equal marginal productivity in some scenarios. One instance is when a job involves team production and perfect substitutes for workers are not readily available. Using a Canadian linked employer-employee survey (2001-2005), we test whether relative wage equals relative marginal productivity among team workers and non-team workers with different frequencies of presenteeism (reduction at work due to illness). For the pooled cross-sectional estimates (2001, 2003, 2005) we obtain 13,755 observations with 6842 unique workplaces. There are 6490 observations for the first differences estimates from the odd years and 5263 observations for the first differences estimates from 2001 to 2002 and 2003 to 2004. We find that in both small and large firms, team workers with frequent reductions at work are less productive but earn similarly compared with non-team workers without reductions. We also find that in small firms, workers with occasional work reductions are more productive than workers without reductions, but the reverse is true in large firms. The study findings partially support the literature stating that productivity loss resulting from employee presenteeism could exceed wages if team work is involved. PMID:26547046

  10. The Elicited Production of Korean Relative Clauses by Heritage Speakers

    Science.gov (United States)

    Lee-Ellis, Sunyoung

    2011-01-01

    In response to new theoretical claims and inconclusive empirical findings regarding relative clauses in East Asian languages, this study examined the factors relevant to relative clause production by Korean heritage speakers. Gap position (subject vs. object), animacy (plus or minus animate), and the topicality of head nouns (plus or minus…

  11. EFSA Panel on Dietetic Products, Nutrition and Allergies (NDA); Scientific Opinion on the substantiation of a health claim related to vitamin K2 and contribution to the normal function of the heart and blood vessels (ID 125, further assessment) pursuant to Article 13(1) of Regulation (EC) No 1924/2006

    DEFF Research Database (Denmark)

    Tetens, Inge

    Following a request from the European Commission, pursuant to Article 13 of Regulation (EC) No 1924/2006, the Panel on Dietetic Products, Nutrition and Allergies was asked to provide a scientific opinion on a health claim related to vitamin K2 and contribution to the normal function of the heart....... In weighing the evidence, the Panel took into account the absence of human intervention studies from which conclusions could be drawn for the scientific substantiation of the claim, the inconsistency of the results reported in two cross-sectional studies regarding arterial calcification in women...

  12. The miRNA Profile of Platelets Stored in a Blood Bank and Its Relation to Cellular Damage from Storage

    OpenAIRE

    Pontes, Thaís Brilhante; Moreira-Nunes, Caroline de Fátima Aquino; Maués, Jersey Heitor da Silva; Lamarão, Letícia Martins; de Lemos, José Alexandre Rodrigues; Montenegro, Raquel Carvalho; Burbano, Rommel Mário Rodriguez

    2015-01-01

    Millions of blood products are transfused each year, and many lives are directly affected by transfusion. Platelet concentrate (PC) is one of the main products derived from blood. Even under good storage conditions, PC is likely to suffer cell damage. The shape of platelets changes after 5 to 7 days of storage at 22°C. Taking into consideration that some platelet proteins undergo changes in their shape and functionality during PC storage. Sixteen PC bags were collected and each PC bag tube wa...

  13. EFSA Panel on Dietetic Products, Nutrition and Allergies (NDA); Scientific Opinion on the substantiation of a health claim related to polyphenols in olive and maintenance of normal blood HDL-cholesterol concentrations (ID 1639, further assessment) pursuant to Article 13(1) of Regulation (EC) No 1924/2006

    DEFF Research Database (Denmark)

    Tetens, Inge

    and maintenance of normal blood HDL-cholesterol concentrations. The food constituent, polyphenols in olive (olive fruit, olive mill waste waters or olive oil, Olea europaea L. extract and leaf) standardised by their content of hydroxytyrosol and its derivatives (e.g. oleuropein complex), that is the...... evidence provided was insufficient to establish a cause and effect relationship between the consumption of olive oil polyphenols (standardised by the content of hydroxytyrosol and its derivatives) and maintenance of normal blood HDL cholesterol concentrations....

  14. EFSA Panel on Dietetic Products, Nutrition and Allergies (NDA); Scientific Opinion on the substantiation of health claims related to L-carnitine and faster recovery from muscle fatigue after exercise (ID 738, 1492, 1493), skeletal muscle tissue repair (ID 738, 1492, 1493), increase in endurance, capacity (ID 4305, 4684), maintenance of normal blood LDL-cholesterol concentrations (ID 1494, 4684), contribution to normal spermatogenesis (ID 1822), “energy metabolism” (ID 1821), and increasing L-carnitine concentrations and/or decreasing free fatty acids in blood during pregnancy (ID 1495) pursuant to

    DEFF Research Database (Denmark)

    Tetens, Inge

    claims in relation to L-carnitine and faster recovery from muscle fatigue after exercise, skeletal muscle tissue repair, increase in endurance capacity, maintenance of normal blood LDL-cholesterol concentrations, contribution to normal spermatogenesis, “energy metabolism”, and increasing L......-carnitine concentrations and/or decreasing free fatty acids in blood during pregnancy. The scientific substantiation is based on the information provided by the Member States in the consolidated list of Article 13 health claims and references that EFSA has received from Member States or directly from stakeholders. The...

  15. Regional cerebral blood flow abnormalities in late-life depression. Relation to refractoriness and chronification

    International Nuclear Information System (INIS)

    We examined patterns of regional cerebral blood flow (rCBF) abnormalities in 18 patients with major depressive disorder in late life using single photon emission computed tomography (SPECT) and 99mTc-hexamethyl-propylenamine oxime (99mTc-HMPAO). Compared with 13 age-matched controls, relative rCBF was significantly decreased bilaterally in the anterior cingulate gyrus, the prefrontal cortex, the temporal cortex, the parietal cortex, the hippocampus and the caudate nucleus. However, it was not correlated with the severity of depression or global cognitive dysfunction. In 10 patients with a prolonged depressive episode or prolonged residual symptoms (the refractory subgroup), robust and extensive decreases in rCBF were found compared with controls and the rCBF decreased significantly in the anterior cingulate gyrus and the prefrontal cortex compared with that in the non-refractory subgroup. In the non-reflactory subgroup, rCBF decreased significantly in the caudate nucleus and tended to decrease in the anterior cingulate gyrus compared with controls. These findings indicate that dysfunction of the limbic system, the cerebral association cortex and the caudate nucleus may be implicated in late-life depression and that robust and extensive hypoperfusion, especially in the anterior cingulate and the prefrontal regions, may relate to refractoriness or chronification of depression. (author). 60 refs

  16. Effects Of Copper Sulphate On Productive, Reproductive Performance And Some Blood Constituents Of Laying Japanese Quail

    International Nuclear Information System (INIS)

    The present study was carried out at the Poultry Research Farm of Nuclear Research Centre, Atomic Energy Authority, Egypt, from February to September 2008 to study the effect of four levels of copper sulphate supplementation (CuSO4,0,200,300 and 400 mg/kg diet) on some of productive and reproductive performance, egg weight, some blood constituents and economical efficiency of laying Japanese quails. Four diets were used from one week of age until mature and egg production stage (30 days age). Body weight was recorded weekly while egg production and egg weight were recorded daily. Resulted eggs were incubated to study the effect of Cu supplementation on fertility, embryonic mortality percentage and hatchability rate. At the end of experiment (6 months of age),10 birds of each group (5 males and 5 females) were slaughtered to study the effect of Cu supplementation on some organs weights such as carcass, liver, heart, gizzard, small intestine, proventricular, ovary, oviduct and tests. Blood components and triiodothyronine (T3) levels were determined. The results showed that body weight was significantly lowered in groups 3 (300 mg) and 4 (400 mg) than group 2 (200 mg). Liver, heart, ovary and oviduct weights were also depressed at the two highest levels of Cu supplementation (groups 3 (300 mg) and 4 (400 mg)). In addition, high level of Cu supplements ≥ 300 mg Cu/kg decreased egg production, hatchability and fertility percentage while they were good or better in group had 200 mg Cu/kg. On the other hand, G2 (200 mg Cu/kg) had the smallest egg weight than other groups. Non-significant effects were observed in serum total protein, albumin and globulin of laying quails had the different levels of CuSO4 except G3 that had the lowest values. Reverse trend was observed in serum triglycerides, cholesterol and triiodothyronine (T3) in groups supplemented high dietary CuSO4. In conclusion, copper is often added to poultry diets at prophylactic concentration of 200 mg

  17. Dysregulation of CXCR3 expression on peripheral blood leukocytes in patients with neovascular age-related macular degeneration

    DEFF Research Database (Denmark)

    Falk, Mads Krüger; Singh, Amardeep; Faber, Carsten; Nissen, Mogens H; Hviid, Thomas; Sørensen, Torben Lykke

    2014-01-01

    Purpose: The chemokine receptor CXCR3 has been strongly related to inhibition of angiogenesis. The purpose of this study was to investigate the association between expression of CXCR3 on peripheral blood leukocytes and Age-related Wet Macular Degeneration (AMD). Furthermore, we measured the plasma...

  18. Localization of CGRP, CGRP receptor, PACAP and glutamate in trigeminal ganglion. Relation to the blood-brain barrier

    DEFF Research Database (Denmark)

    Eftekhari, Sajedeh; Salvatore, Christopher A; Johansson, Sara;

    2015-01-01

    ) and related this to the expression of CGRP and its receptor in rhesus trigeminal ganglion. Pituitary adenylate cyclase-activating polypeptide (PACAP) and glutamate were examined and related to the CGRP system. Furthermore, we examined if the trigeminal ganglion is protected by the blood-brain barrier...

  19. Mortality and cancer in relation to ABO blood group phenotypes in the Golestan Cohort Study

    OpenAIRE

    Etemadi, Arash; Kamangar, Farin; Islami, Farhad; Poustchi, Hossein; Pourshams, Akram; Brennan, Paul; Boffetta, Paolo; Malekzadeh, Reza; Dawsey, Sanford M.; Abnet, Christian C.; Emadi, Ashkan

    2015-01-01

    Background A few studies have shown an association between blood group alleles and vascular disease, including atherosclerosis, which is thought to be due to the higher level of von Willebrand factor in these individuals and the association of blood group locus variants with plasma lipid levels. No large population-based study has explored this association with overall and cause-specific mortality. Methods We aimed to study the association between ABO blood groups and overall and cause-specif...

  20. Assessment of weather-related risk on chestnut productivity

    Directory of Open Access Journals (Sweden)

    M. G. Pereira

    2011-10-01

    Full Text Available Due to its economic and nutritional value, the world production of chestnuts is increasing as new stands are being planted in various regions of the world. This work focuses on the relation between weather and annual chestnut production to model the role of weather, to assess the impacts of climate change and to identify appropriate locations for new groves. The exploratory analysis of chestnut production time series and the striking increase of production area have motivated the use for chestnut productivity. A large set of meteorological variables and remote sensing indices were computed and their role on chestnut productivity evaluated with composite and correlation analyses. These results allow for the identification of the variables cluster with a high correlation and impact on chestnut production. Then, different selection methods were used to develop multiple regression models able to explain a considerable fraction of productivity variance: (i a simulation model (R2-value = 87% based on the winter and summer temperature and on spring and summer precipitation variables; and, (ii a model to predict yearly chestnut productivity (R2-value of 63% with five months in advance, combining meteorological variables and NDVI. Goodness of fit statistic, cross validation and residual analysis demonstrate the model's quality, usefulness and consistency of obtained results.

  1. Effect of guar crispbread with cereal products and leguminous seeds on blood glucose concentrations of diabetics.

    Science.gov (United States)

    Jenkins, D J; Wolever, T M; Taylor, R H; Barker, H M; Fielden, H; Jenkins, A L

    1980-11-01

    To compare the effect on blood glucose concentrations of guar incorporated into crispbreads with that of unprocessed high-fibre foods groups of four to six diabetics took a total of seven test breakfasts on separate days. By comparison with a breakfast of wholemeal bread and cheese, guar crispbread combined with bread reduced the area under the glucose response curve to 51% (p soya beans reduced the area to 65% (p soya beans to 25% (p soya beans with lentils to 29% (p < 0.002). Porridge and cornflake breakfasts showed no difference. The favourable results with leguminous seeds may not make such meals more acceptable than meals of guar products, but a combination of leguminous seeds and guar may allow smaller and more acceptable amounts of both to be used. PMID:6253021

  2. RFID in the blood supply chain--increasing productivity, quality and patient safety.

    Science.gov (United States)

    Briggs, Lynne; Davis, Rodeina; Gutierrez, Alfonso; Kopetsky, Matthew; Young, Kassandra; Veeramani, Raj

    2009-01-01

    As part of an overall design of a new, standardized RFID-enabled blood transfusion medicine supply chain, an assessment was conducted for two hospitals: the University of Iowa Hospital and Clinics (UIHC) and Mississippi Baptist Health System (MBHS). The main objectives of the study were to assess RFID technological and economic feasibility, along with possible impacts to productivity, quality and patient safety. A step-by-step process analysis focused on the factors contributing to process "pain points" (errors, inefficiency, product losses). A process re-engineering exercise produced blueprints of RFID-enabled processes to alleviate or eliminate those pain-points. In addition, an innovative model quantifying the potential reduction in adverse patient effects as a result of RFID implementation was created, allowing improvement initiatives to focus on process areas with the greatest potential impact to patient safety. The study concluded that it is feasible to implement RFID-enabled processes, with tangible improvements to productivity and safety expected. Based on a comprehensive cost/benefit model, it is estimated for a large hospital (UIHC) to recover investment from implementation within two to three years, while smaller hospitals may need longer to realize ROI. More importantly, the study estimated that RFID technology could reduce morbidity and mortality effects substantially among patients receiving transfusions. PMID:19894488

  3. Evaluation of relational database products for the VAX

    International Nuclear Information System (INIS)

    Four commercially available database products for the VAX/VMS operating system were evaluated for relative performance and ease of use. The products were DATATRIEVE, INGRES, Rdb, and S1032. Performance was measured in terms of elapsed time, CPU time, direct I/O counts, buffered I/O counts, and page faults. East of use is more subjective and has not been quantified here; however, discussion and tables of features as well as query syntax are included. This report describes the environment in which these products were evaluated and the characteristics of the databases used. All comparisons must be interpreted in the context of this setting

  4. Detection of blood-related signal from a series of fingerprint images acquired during an input action

    Science.gov (United States)

    Fujieda, Ichiro; Hori, Atsushi; Kurita, Masashi

    2007-09-01

    When a finger is pressed against a flat plate and deformed, blood inside the finger moves away from the deformed area. This causes the finger to change its appearance from reddish to white. As the finger leaves the plate, the blood comes back and it looks reddish again. We have proposed to use this color change to distinguish genuine fingers from artificial ones for un-attended fingerprint identification systems. This blood-related signal may reflect the stiffness of the peripheral blood vessels and therefore it may be correlated with some health conditions such as blood pressure. In experiments, we used a fingerprint sensor based on scattered light detection. Because the spectra of the light scattered by the deformed fingers showed large changes mostly in the green portion, an LED emitting at 525 nm at peak strength was used. First, we compared series of fingerprint images acquired during a normal input action and those obtained while a rubber band occluded the blood flow. The occluded finger required a larger force to exhibit a similar change for these pixel values than the finger without the rubber band. Second, we analyzed fingerprint images recorded by six volunteers. We defined some indices based on the pixel values of the fingerprint images and the pressure applied to the fingers. The correlation coefficient of one of such indices and the average blood pressure of the participants was 0.86. Although the number of the subjects is small, this initial result is encouraging.

  5. Corrigendum - Effect of live yeast culture Saccharomyces cerevisiae on milk production and some blood parameters

    Directory of Open Access Journals (Sweden)

    Judit Peter Szucs

    2014-05-01

    Full Text Available In the article Effect of live yeast culture Saccharomyces cerevisiae on milk production and some blood parameters, first published in 2013 in Scientific Papers: Animal Science and Biotechnologies, 46 (1, the first author neglected to ask any prior agreement and permission of Lesaffre Feed Additive firm the sponsor of the experiment and Bernhard Feix GmbH the leader of the experiment, for publishing the results, doing so she committed serious mistakes: Ethically: The first author published data for which she had no permission, and she did not indicate the name of the owners, neither did she refere to them. On the scientific basis: She was not able to evaluate properly the real effect of Saccharomices cerevisiae containing Actisaf additive since the introduced experiment was a basic large farm size /scale research work that could not be measured precisely without the planned and ongoing further experiments.   Acknowledgement The first author highly appreciates Lesaffre Feed Additive and Bernhard Feix GmbH firms for the possibility of taking part in the research work together with her students. She is most grateful for the generous promise of the leaders of both Lesaffre Feed Additive and Bernhard Feix GmbH for disregarding to take any legal steps. She gives thanks especially to the leader of the experiment Zoltan Pachinger for his wise and helpful contribution to this consensus. The first author apologises for this oversight.     This article corrects: Effect of live yeast culture Saccharomyces cerevisiae on milk production and some blood parameters, Vol. 46, Issue 1, p. 40-44. Article first published online: 30 May 2013

  6. The effect of chronic peripheral nesfatin-1 application on blood pressure in normal and chronic restraint stressed rats: related with circulating level of blood pressure regulators.

    Science.gov (United States)

    Ayada, Ceylan; Turgut, Günfer; Turgut, Sebahat; Güçlü, Zuhal

    2015-01-01

    Nesfatin is a peptide secreted by peripheral tissues, central and peripheral nervous system. It is involved in the regulation of homeostasis. Although the effects of nesfatin-1 on nutrition have been studied widely in the literature, the mechanisms of nesfatin-1 action and also relations with other physiological parameters are still not clarified well. We aimed to investigate the effect of peripheral chronic nesfatin-1 application on blood pressure regulation in normal and in rats exposed to restraint immobilization stress. In our study, three month-old male Wistar rats were used. Rats were divided into 4 groups as Control, Stress, Control+Nesfatin-1, Nesfatin-1+Stress. Angiotensinogen, angiotensin converting enzyme 2, angiotensin II, endothelin-1, endothelial nitric oxide synthase, aldosterone, cortisol, nesfatin-1 levels were determined in plasma samples by ELISA. Our results have shown that chronic peripheral nesfatin-1 administration increases blood pressure in normal and in rats exposed to chronic restraint stress. Effect of nesfatin-1 on circulating level of angiotensinogen, angiotensin converting enzyme 2, angiotensin II, endothelin-1, endothelial nitric oxide synthase, aldosterone and cortisol has been identified. We can conclude that elevated high blood pressure after chronic peripheral nesfatin-1 administration in rats exposed to chronic restraint stress may be related to decreased plasma level of endothelial nitric oxide synthase concentration. PMID:25504061

  7. EFSA Panel on Dietetic Products, Nutrition and Allergies (NDA); Scientific Opinion on the substantiation of a health claim related to isoleucyl-prolyl-proline (IPP) and valyl-prolyl-proline (VPP) and maintenance of normal blood pressure pursuant to Article 13(5) of Regulation (EC) No 1924/2006

    DEFF Research Database (Denmark)

    Tetens, Inge

    Following an application from Valio Ltd submitted pursuant to Article 13(5) of Regulation (EC) No 1924/2006 via the Competent Authority of Finland, the Panel on Dietetic Products, Nutrition and Allergies was asked to deliver an opinion on the scientific substantiation of a health claim related to...... studies (in 19 papers; five papers were translated by the applicant into English from the Japanese original) and one unpublished study, as well as two published and one unpublished meta-analyses of randomised controlled trials (RCTs) as being pertinent to the claim. Thirteen of the RCTs provided, four of...... methodological limitations. The animal studies did not provide additional information on the effect of IPP and VPP on BP in humans, and no convincing evidence for a mechanism by which IPP and VPP could exert the claimed effect at the proposed dose has been provided. The Panel concludes that a cause and effect...

  8. Whole Blood Activation Results in Enhanced Detection of T Cell and Monocyte Cytokine Production by Flow Cytometry

    Science.gov (United States)

    Sams, Clarence F.; Crucian, Brian E.

    2001-01-01

    An excellent monitor of the immune balance of peripheral circulating cells is to determine their cytokine production patterns in response to stimuli. Using flow cytometry a positive identification of cytokine producing cells in a mixed culture may be achieved. Recently, the ability to assess cytokine production following a wholeblood activation culture has been described. We compared whole blood culture to standard PBMC culture and determined the individual cytokine secretion patterns for both T cells and monocytes via flow cytometry. For T cells cytokine assessment following PMA +ionomycin activation: (1) a significantly greater percentages of T cells producing IFNgamma and IL-2 were observed following whole-blood culture; (2) altered T cell cytokine production kinetics were observed by varying whole blood culture times. In addition, a four-color cytometric analysis was used to allow accurate phenotyping and quantitation of cytokine producing lymphocyte populations. Using this technique we found IFNgamma production to be significantly elevated in the CD3+/CD8+ T cell population as compared to the CD3+/CD8- population following five hours of whole blood activation. Conversely, IL-2 and IL-10 production were significantly elevated in the CD3+/CD8- T cell population as compared to the CD3+/CD8+ population. Monocyte cytokine production was assessed in both culture systems following LPS activation for 24 hours. A three-color flow cytometric was used to assess two cytokines in conjunction with CD 14. The cytokine pairs used for analysis were IL-1a/IL-12, and IL-10ITNFa. Nearly all monocytes were stimulated to produce IL-1a, IL-12 and TNFalpha equally well in both culture systems. Monocyte production of IL-10 was significantly elevated following whole blood culture as compared to PBMC culture. IL-12 producing monocytes appeared to be a distinct subpopulation of the IL-1a producing set, whereas IL-10 and TNFa producing monocytes were largely mutually exclusive. IL-10 and

  9. Decomposing one-relator products of cyclic groups into free products with amalgamation

    International Nuclear Information System (INIS)

    The problem of the decomposition of one-relator products of cyclics into non-trivial free products with amalgamation is considered. Two theorems are proved, one of which is as follows. Let G=2n=Rm(a,b)=1>, where n≥0, m≥2, and R(a,b) is a cyclically reduced word containing b in the free group on a and b. Then G is a non-trivial free product with amalgamation. One consequence of this theorem is a proof of the conjecture of Fine, Levin, and Rosenberger that each two-generator one-relator group with torsion is a non-trivial free product with amalgamation

  10. Expression of P53(v) protein of peripheral blood in patients with tumor and its relation with age

    International Nuclear Information System (INIS)

    To study the expression of P53(v) protein of peripheral blood monocytes in cancer patients and its relation with age, P53(v) proteins were determined in 88 cancer patients and 88 normals by flow cytometry. The levels of P53(v) were (7.76 +- 7.13)% in cancer group and (0.66 +- 0.5)% in normal group. Mean value of P53(v) in the cancers was higher than that in the controls (P<0.01). The expression of P53(v) of peripheral blood raised with age. Conclusion: P53(v) proteins can be detected in peripheral blood monocytes. The detection of P53 expression in human peripheral blood monocytes can be used to screen high risk population with tumor

  11. Spleen size changes in children with homozygous. beta. -thalassaemia in relation to blood transfusion

    Energy Technology Data Exchange (ETDEWEB)

    Karpathios, Th.; Antypas, A.; Dimitriou, P.; Nicolaidou, P.; Fretzayas, A.; Thomaidis, Th.; Matsaniotis, N. (First Department of Paediatrics of Athens University, Aghia Sophia Children' s Hospital, Athens, Greece and Department of Pathologic Physiology, University of Athens, Athens, Greece)

    1982-01-01

    18 thalassaemic children, aged 3.5 to 13 years comprise our clinical material. In 14 of them, clinically elicited spleen markings, haematocrit, blood platelet count and red cell morphology were studied daily for a whole period between 2 transfusions. In 10 patients considerable changes in spleen size were noticed. According to our clinical observations the spleen size starts decreasing 1 to 3 d after blood transfusion up to the 10th posttransfusion day fluctuating thereafter to reach its maximum size again prior to the next blood transfusion. The decrease of spleen size was followed by an increase of haematocrit and blood platelet count and vice versa. 4 additional children were studied clinically only twice: prior to and 7 to 10 d after blood transfusion. A definite decrease of the spleen size following blood transfusion was observed. Spleen and liver sup(99m)Tc-sulfur colloid uptake was studied in 10 of the above children prior to and 7 to 10 d after blood transfusion. Statistically significant post-transfusion increase of the spleen uptake was demonstrated. Our findings suggest that (a) splenic size is relevant to blood volume sequestered in this organ, (b) splenic radioactive uptake increases with its post-transfusion reduction in size.

  12. Spleen size changes in children with homozygous β-thalassaemia in relation to blood transfusion

    International Nuclear Information System (INIS)

    18 thalassaemic children, aged 3.5 to 13 years comprise our clinical material. In 14 of them, clinically elicited spleen markings, haematocrit, blood platelet count and red cell morphology were studied daily for a whole period between 2 transfusions. In 10 patients considerable changes in spleen size were noticed. According to our clinical observations the spleen size starts decreasing 1 to 3 d after blood transfusion up to the 10th posttransfusion day fluctuating thereafter to reach its maximum size again prior to the next blood transfusion. The decrease of spleen size was followed by an increase of haematocrit and blood platelet count and vice versa. 4 additional children were studied clinically only twice: prior to and 7 to 10 d after blood transfusion. A definite decrease of the spleen size following blood transfusion was observed. Spleen and liver sup(99m)Tc-sulfur colloid uptake was studied in 10 of the above children prior to and 7 to 10 d after blood transfusion. Statistically significant post-transfusion increase of the spleen uptake was demonstrated. Our findings suggest that (a) splenic size is relevant to blood volume sequestrated int this organ, (b) splenic radioactive uptake increases with its post-transfusion reductin in size. (author)

  13. Effect of Korea red ginseng on cerebral blood flow and superoxide production

    Institute of Scientific and Technical Information of China (English)

    Cuk Seong KIM; Jin Bong PARK; Kwang-Jin KIM; Seok Jong CHANG; Sung-Woo RYOO; Byeong Hwa JEON

    2002-01-01

    AIM: To investigate the effects of Korea red ginseng (KRG) on the cerebral perfusion rate in the rats and the generation of superoxide anion in the endothelial cells. METHODS: The cerebral perfusion rate was measured using laser-doppler flowmetry before and after the administration of crude saponin (CS) and saponin-free fraction (SFF) of KRG in the anesthetized rats. The superoxide generation was measured by the method based on lucigeninenhanced chemiluminescence in the cultured endothelial cells. RESULTS: The relative cerebral perfusion rate (rCBF) was significantly increased by the intraperitoneal injection of CS (100 mg/kg) in the rats, but SFF had no effect on the rCBF. Chronic treatment with CS for 7 d significantly inhibited the decrease of forebrain cerebral blood flow induced by clamping both carotid arteries in the rats. Furthermore, CS (0.1 g/L) significantly suppressed NADPH-induced superoxide generation in the human umbilical vein endothelial cells (P<0.01).CONCLUSION: The present study demonstrated that crude saponin fraction of KRG enhanced cerebral blood flow in rats. Furthermore, crude saponin fraction of KRG abrogated the NADPH-driven superoxide generation in endothelial cells.

  14. Limiting excessive postoperative blood transfusion after cardiac procedures. A review.

    OpenAIRE

    Ferraris, V A; Ferraris, S P

    1995-01-01

    Analysis of blood product use after cardiac operations reveals that a few patients ( 80%). The risk factors that predispose a minority of patients to excessive blood use include patient-related factors, transfusion practices, drug-related causes, and procedure-related factors. Multivariate studies suggest that patient age and red blood cell volume are independent patient-related variables that predict excessive blood product transfusion aft...

  15. A proposal for a classification of product-related dependencies in development of mechatronic products

    DEFF Research Database (Denmark)

    Torry-Smith, Jonas; Mortensen, Niels Henrik; Achiche, Sofiane

    2014-01-01

    When designing mechatronic products ‘complex dependencies’ are often reported to be a major challenge. This paper focuses on managing dependencies between attributes of the product during the design process. The literature study shows that there is a gap in the literature with regards to the...... classification of product-related dependencies. Traditionally these dependencies have been described as appearing between the following product attributes: function, properties and structure. By analysing three mechatronic projects from industry we identified and classified 13 types of product...

  16. Beneficial effect of donor-specific blood transfusions (DST on living-related kidney allograft survival.

    Directory of Open Access Journals (Sweden)

    Sakagami,Kenichi

    1986-02-01

    Full Text Available The survival rate of 19 patients who underwent living-related kidney transplantation after donor-specific blood transfusions (DST was compared with that of 32 historical controls receiving transplants without DST. The graft survival rate of the DST group was 82% after two and three years. The graft survival rate of the DST group was significantly better than the 53% rate after two years obtained with the 32 historical controls (p less than 0.05. We tested sera from 16 DST-treated recipients to study the beneficial effect of DST on kidney allograft survival using the mixed lymphocyte culture (MLC serum inhibition test. The results demonstrated that MLC inhibitory factors were induced in the serum of the recipient after completion of DST. This inhibition of MLC was observed by treatment of responder lymphocytes with serum obtained three weeks after DST plus rabbit complement. The inhibitory effect was also specific for responder cells in anti-donor MLC. Regarding the correlation with rejection episodes, these MLC inhibitory factors were often observed in the non-rejection group (p less than 0.05. The data suggest that such factors may be anti-idiotypic antibodies and be associated with prolonged graft survival.

  17. Effect of anticoagulant administration on blood clotting and some hormones related to rat-fertility

    International Nuclear Information System (INIS)

    This study was performed using 30 mature male albino rats divided into 3 equal groups; control and two treated groups to assess the effect of anticoagulant (warfarin) administration on the level of some hormones related to fertility. The two treated groups were injected intraperitoneally every other day with 1 ml (0.03 mg)and 2 ml (0.06 mg)warfarin/ 100 g body weight respectively where, two specimens were taken from each group after two and four weeks. Clotting time (CT), prothrombin time (PT), partial prothrombin time (PTT) platelets count, fasting blood sugar (F.B.S), calcium levels in addition to triiodothyronine (T3), thyroxin (T4), insulin, corticosterone, and testosterone hormones were determined. The results showed that the intraperitoneal injection of warfarin caused significant increase in clotting time, prothrombin time , partial prothrombin time, platelets count and glucose level, while serum calcium level showed significant decrease. Intraperitoneal injection of warfarin caused significant decrease of insulin and significant increase of corticosterone, T3 showed significant decrease in high dose group while T4 showed significant decrease in small dose group. The high dose was associated with the highest level of testosterone hormone. these results denoted that warfarin anticoagulant had no negative effect on gonadal sex hormone and hence on male fertility

  18. Reduction in Cerebral Oxygenation After Prolonged Exercise in Hypoxia is Related to Changes in Blood Pressure.

    Science.gov (United States)

    Horiuchi, Masahiro; Dobashi, Shohei; Kiuchi, Masataka; Endo, Junko; Koyama, Katsuhiro; Subudhi, Andrew W

    2016-01-01

    We investigated the relation between blood pressure and cerebral oxygenation (COX) immediately after exercise in ten healthy males. Subjects completed an exercise and recovery protocol while breathing either 21% (normoxia) or 14.1% (hypoxia) O2 in a randomized order. Each exercise session included four sets of cycling (30 min/set, 15 min rest) at 50% of altitude-adjusted peak oxygen uptake, followed by 60 min of recovery. After exercise, mean arterial pressure (MAP; 87±1 vs. 84±1 mmHg, average values across the recovery period) and COX (68±1% vs. 58±1%) were lower in hypoxia compared to normoxia (Pblood pressure following exercise in hypoxia are (1) more pronounced than in normoxia, and (2) associated with reductions in COX. Together, these results suggest an impairment in cerebral autoregulation as COX followed changes in MAP more passively in hypoxia than in normoxia. These findings could help explain the increased risk for postexercise syncope at high altitude. PMID:26782200

  19. An integrative evolution theory of histo-blood group ABO and related genes.

    Science.gov (United States)

    Yamamoto, Fumiichiro; Cid, Emili; Yamamoto, Miyako; Saitou, Naruya; Bertranpetit, Jaume; Blancher, Antoine

    2014-01-01

    The ABO system is one of the most important blood group systems in transfusion/transplantation medicine. However, the evolutionary significance of the ABO gene and its polymorphism remained unknown. We took an integrative approach to gain insights into the significance of the evolutionary process of ABO genes, including those related not only phylogenetically but also functionally. We experimentally created a code table correlating amino acid sequence motifs of the ABO gene-encoded glycosyltransferases with GalNAc (A)/galactose (B) specificity, and assigned A/B specificity to individual ABO genes from various species thus going beyond the simple sequence comparison. Together with genome information and phylogenetic analyses, this assignment revealed early appearance of A and B gene sequences in evolution and potentially non-allelic presence of both gene sequences in some animal species. We argue: Evolution may have suppressed the establishment of two independent, functional A and B genes in most vertebrates and promoted A/B conversion through amino acid substitutions and/or recombination; A/B allelism should have existed in common ancestors of primates; and bacterial ABO genes evolved through horizontal and vertical gene transmission into 2 separate groups encoding glycosyltransferases with distinct sugar specificities. PMID:25307962

  20. Modification of integration relations of subjects of innovation production

    Directory of Open Access Journals (Sweden)

    Vaganova Oksana Valerievna

    2012-12-01

    Full Text Available The necessity of shifting the economy to the innovative way of development caused the research of the features of functioning of modern science-intensive production. The actual existing prerequisites for the consolidation of such manufactures into the integration structures are revealed; the necessity and the expediency of setting up integration relations are proved.

  1. Relational dynamics in the multi-helices knowledge production system

    DEFF Research Database (Denmark)

    Thai, Thi Minh; Hjortsø, Carsten Nico Portefée

    emerging economy. Findings highlight the emergence of a fifth-helices knowledge production system includes the state, science and education, industry, international actors, and society. The system comprises two major segments, one associated with the traditional command economy and characterized by......-level dynamics are characterized by political ambidexterity that enables the state to maintain control by privileging traditional science and education constituencies, and at the same time support the transition of the knowledge production system towards international methodology and quality standards through......Drawing on the triple helix framework and organizational institutionalism, this article applies a qualitative research approach to analyze structures, institutional logics, power relations that shape inter-organizational relations and the structuration of a knowledge production system in an...

  2. EFSA NDA Panel (EFSA Panel on Dietetic Products, Nutrition and Allergies), 2014. Scientific Opinion on the substantiation of a health claim related to barley soup “Orzotto” and protection of blood lipids from oxidative damage pursuant to Article 13(5) of Regulation (EC) No 1924/2006

    DEFF Research Database (Denmark)

    Tetens, Inge

    coloured” spinach, “tri-coloured” Swiss chard, onions, red and yellow celery and parsley, is sufficiently characterised. The claimed effect, protection of blood lipids from oxidative damage, may be a beneficial physiological effect. No human intervention studies from which conclusions could be drawn for...

  3. Do distribution volumes and clearances relate to tissue volumes and blood flows? A computer simulation

    Directory of Open Access Journals (Sweden)

    Lemmens Hendrikus JM

    2006-06-01

    Full Text Available Abstract Background Kinetics of inhaled agents are often described by physiological models. However, many pharmacokinetic concepts, such as context-sensitive half-times, have been developed for drugs described by classical compartmental models. We derived classical compartmental models that describe the course of the alveolar concentrations (FA generated by the physiological uptake and distribution models used by the Gas Man® program, and describe how distribution volumes and clearances relate to tissue volumes and blood flows. Methods Gas Man® was used to generate FA vs. time curves during the wash-in and wash-out period of 115 min each with a high fresh gas flow (8 L.min-1, a constant alveolar minute ventilation (4 L.min-1, and a constant inspired concentration (FI of halothane (0.75%, isoflurane (1.15%, sevoflurane (2%, or desflurane (6%. With each of these FI, simulations were ran for a 70 kg patient with 5 different cardiac outputs (CO (2, 3, 5, 8 and 10 L.min-1 and for 5 patients with different weights (40, 55, 70, 85, and 100 kg but the same CO (5 L.min-1. Two and three compartmental models were fitted to FA of the individual 9 runs using NONMEM. After testing for parsimony, goodness of fit was evaluated using median prediction error (MDPE and median absolute prediction error (MDAPE. The model was tested prospectively for a virtual 62 kg patient with a cardiac output of 4.5 L.min-1 for three different durations (wash-in and wash-out period of 10, 60, and 180 min each with an FI of 1.5% halothane, 1.5% isoflurane, sevoflurane 4%, or desflurane 12%. Results A three-compartment model fitted the data best (MDPE = 0% and MDAPE ≤ 0.074% and performed equally well when tested prospectively (MDPE ≤ 0.51% and MDAPE ≤ 1.51%. The relationship between CO and body weight and the distribution volumes and clearances is complex. Conclusion The kinetics of anesthetic gases can be adequately described e by a mammilary compartmental model. Therefore

  4. Relationships between human vitality and mitochondrial respiratory parameters, reactive oxygen species production and dNTP levels in peripheral blood mononuclear cells

    DEFF Research Database (Denmark)

    Maynard, Scott; Keijzers, Guido; Gram, Martin; Desler, Claus; Bendix, Laila; Budtz-Jørgensen, Esben; Molbo, Drude; Croteau, Deborah L; Osler, Merete; Stevnsner, Tinna V.; Rasmussen, Lene Juel; Dela, Flemming; Avlund, Kirsten; Bohr, Vilhelm

    2013-01-01

    . Therefore, we measured a number of cellular parameters related to mitochondrial activity in peripheral blood mononuclear cells (PBMCs) isolated from middle-aged men, and tested for association with vitality. These parameters estimate mitochondrial respiration, reactive oxygen species (ROS) production, and......Low vitality (a component of fatigue) in middle-aged and older adults is an important complaint often identified as a symptom of a disease state or side effect of a treatment. No studies to date have investigated the potential link between dysfunctional mitochondrial ATP production and low vitality...

  5. Relationships between human vitality and mitochondrial respiratory parameters, reactive oxygen species production and dNTP levels in peripheral blood mononuclear cells

    DEFF Research Database (Denmark)

    Maynard, Scott; Keijzers, Guido; Gram, Martin; Madsen, Claus Desler; Bendix, Laila; Budtz-Jørgensen, Esben; Molbo, Drude; Croteau, Deborah L; Osler, Merete; Stevnsner, Tinna V.; Rasmussen, Lene Juel; Dela, Flemming; Avlund, Kirsten; Bohr, Vilhelm A

    . Therefore, we measured a number of cellular parameters related to mitochondrial activity in peripheral blood mononuclear cells (PBMCs) isolated from middle-aged men, and tested for association with vitality. These parameters estimate mitochondrial respiration, reactive oxygen species (ROS) production, and......Low vitality (a component of fatigue) in middle-aged and older adults is an important complaint often identified as a symptom of a disease state or side effect of a treatment. No studies to date have investigated the potential link between dysfunctional mitochondrial ATP production and low vitality...

  6. Measuring the relative productivity of multitasking to sole-tasking in household production: New experimental evidence

    OpenAIRE

    Foster, Gigi; Kalenkoski, Charlene

    2012-01-01

    We present a household production model that incorporates multitasking and results from a customized experiment designed to measure the individual-specific productivity parameters from this model. We observe these productivity parameters under alternative incentive scenarios, designed to mimic changes in the relative utility payoffs to the two tasks. Using information on demographic and other characteristics collected through an on-screen survey taken by our experimental participants, we exam...

  7. Structural analysis of the RH-like blood group gene products in nonhuman primates

    Energy Technology Data Exchange (ETDEWEB)

    Salvignol, I. [Centre Regional de Transfusion Sanguine, Toulouse (France); Calvas, P.; Blancher, A. [Universitaire d`Immunogenetique moleculaire, Toulouse (France); Socha, W.W. [University Medical Center, New York, NY (United States); Colin, Y.; Le Van Kim, C.; Bailly, P.; Cartron, J.P. [Institut National de la Transfusion Sanguine, Paris (France); Ruffie, J.; Blancher, A. [College de France, Paris (France)

    1995-03-01

    Rh-related transcripts present in bone marrow samples from several species of nonhuman primates (chimpanzee, gorilla, gibbon, crab-eating macaque) have been amplified by RT-polymerase chain reaction using primers deduced from the sequence of human RH genes. Nucleotide sequence analysis of the nonhuman transcripts revealed a high degree of similarity to human blood group Rh sequences, suggesting a great conservation of the RH genes throughout evolution. Full-length transcripts, potentially encoding 417 amino acid long proteins homologous to Rh polypeptides, were characterized, as well as mRNA isoforms which harbored nucleotide deletions or insertions and potentially encode truncated proteins. Proteins of 30-40,000 M{sub r}, immunologically related to human Rh proteins, were detected by western blot analysis with antipeptide antibodies, indicating that Rh-like transcripts are translated into membrane proteins. Comparison of human and nonhuman protein sequences was pivotal in clarifying the molecular basis of the blood group C/c polymorphism, showing that only the Pro103Ser substitution was correlated with C/c polymorphism. In addition, it was shown that a proline residue at position 102 was critical in the expression of C and c epitopes, most likely by providing an appropriate conformation of Rh polypeptides. From these data a phylogenetic reconstruction of the RH locus evolution has been calculated from which an unrooted phylogenetic tree could be proposed, indicating that African ape Rh-like genes would be closer to the human RhD gene than to the human RhCE gene. 55 refs., 4 figs., 1 tab.

  8. Straight versus tortuous retinal arteries in relation to blood pressure and genetics

    DEFF Research Database (Denmark)

    Taarnhøj, N C B B; Munch, I C; Sander, B;

    2008-01-01

    dizygotic same-sex healthy twin pairs, aged 20 to 46 years, who were characterised by determination of retinal vessel diameters, arterial blood pressure, blood glucose, body mass index, smoking habits and retinal arterial tortuosity, using a three-level grading scale (straight, wavy, tortuous). Heritability...... factors accounting for the remaining 18% (CI(95 )8, 36%). Increasing values of mean arterial blood pressure and body mass index were both associated with decreasing levels of retinal arterial tortuosity. CONCLUSION: There was a large variation in tortuosity of retinal arteries in these healthy subjects...

  9. Cafeteria diet induce changes in blood flow that are more related with heat dissipation than energy accretion

    Science.gov (United States)

    Sabater, David; Agnelli, Silvia; Arriarán, Sofía; Romero, María del Mar; Fernández-López, José Antonio; Alemany, Marià

    2016-01-01

    Background. A “cafeteria” diet is a self-selected high-fat diet, providing an excess of energy, which can induce obesity. Excess of lipids in the diet hampers glucose utilization eliciting insulin resistance, which, further limits amino acid oxidation for energy. Methods. Male Wistar rats were exposed for a month to “cafeteria” diet. Rats were cannulated and fluorescent microspheres were used to determine blood flow. Results. Exposure to the cafeteria diet did not change cardiac output, but there was a marked shift in organ irrigation. Skin blood flow decreased to compensate increases in lungs and heart. Blood flow through adipose tissue tended to increase in relation to controls, but was considerably increased in brown adipose tissue (on a weight basis). Discussion. The results suggest that the cafeteria diet-induced changes were related to heat transfer and disposal. PMID:27547590

  10. Penile rehabilitation with a vacuum erectile device in an animal model is related to an antihypoxic mechanism: blood gas evidence.

    Science.gov (United States)

    Lin, Hao-Cheng; Yang, Wen-Li; Zhang, Jun-Lan; Dai, Yu-Tian; Wang, Run

    2013-05-01

    Our previous study showed that vacuum erectile device (VED) therapy has improved erectile function in rats with bilateral cavernous nerve crush (BCNC) injuries. This study was designed to explore the mechanism of VED in penile rehabilitation by analyzing cavernous oxygen saturation (SO2) and to examine the effect of VED therapy on preventing penile shrinkage after BCNC. Thirty adult Sprague-Dawley rats were randomly assigned into three groups: group 1, sham surgery; group 2, BCNC; and group 3, BCNC+VED. Penile length and diameter were measured on a weekly basis. After 4 weeks of therapy, the penile blood was extracted by three methods for blood gas analysis (BGA): method 1, cavernous blood was aspirated at the flaccid state; method 2, cavernous blood was aspirated at the traction state; and method 3, cavernous blood was aspirated immediately after applying VED. SO2 values were tested by the blood gas analyzer. The results showed that VED therapy is effective in preventing penile shrinkage induced by BCNC (Penile shortening: BCNC group 1.9±1.1 mm; VED group 0.3±1.0 mm; PVED group 0.04±0.14 mm; PVED application (88.25%±4.94%) compared to the flaccid (76.53%±4.16%) or traction groups (78.93%±2.56%) (PVED application were 62% arterial and 38% venous blood. These findings suggest that VED therapy can effectively preserve penile size in rats with BCNC injury. The beneficial effect of VED therapy is related to antihypoxia by increasing cavernous blood SO2. PMID:23564044

  11. Markers of collagen synthesis is related to blood pressure and vascular hypertrophy: a LIFE substudy

    DEFF Research Database (Denmark)

    Olsen, M H; Christensen, M K; Wachtell, K;

    2005-01-01

    Cardiac fibrosis and high levels of circulating collagen markers has been associated with left ventricular (LV) hypertrophy. However, the relationship to vascular hypertrophy and blood pressure (BP) load is unclear. In 204 patients with essential hypertension and electrocardiographic LV hypertrophy...

  12. Related Hematopoietic Stem Cell Transplantation (HSCT) for Genetic Diseases of Blood Cells

    Science.gov (United States)

    2016-05-11

    Stem Cell Transplantation; Bone Marrow Transplantation; Peripheral Blood Stem Cell Transplantation; Allogeneic Transplantation,; Genetic Diseases; Thalassemia; Pediatrics; Diamond-Blackfan Anemia; Combined Immune Deficiency; Wiskott-Aldrich Syndrome; Chronic Granulomatous Disease; X-linked Lymphoproliferative Disease; Metabolic Diseases

  13. Blood vessel growth blocker may treat AIDS-related Kaposi’s sarcoma

    Science.gov (United States)

    Patients with an AIDS-associated cancer, Kaposi's sarcoma (KS), showed improvement after receiving the combination of bevacizumab, a cancer drug that blocks the growth of new blood vessels, and highly active antiretroviral therapy (HAART).

  14. Genetic and phenotypic relationships between blood gas parameters and ascites-related traits in broilers.

    Science.gov (United States)

    Closter, A M; van As, P; Groenen, M A M; Vereijken, A L J; van Arendonk, J A M; Bovenhuis, H

    2009-03-01

    Ascites, also called pulmonary hypertension syndrome, is a metabolic disorder in chickens that have an insufficient pulmonary vascular capacity. The tendency of broilers to develop ascites is heritable, and successful selection against this susceptibility would benefit from good and easy-to-measure indicator traits. Blood gas parameters have been suggested as indicator traits for ascites susceptibility. Therefore, the aim of the present study was to estimate the heritability of blood gas parameters and the genetic and phenotypic correlations between blood gas parameters, heart ratio (postmortem indicator for ascites), and BW at 2 different ages. For this purpose, blood gas parameters, including the partial pressure of carbon dioxide in venous blood (pvCO(2)), the partial pressure of oxygen in venous blood (pvO(2)), and blood oxygen saturation, were measured at an average age of 22 d in nearly 3,000 broilers. To challenge the resistance of the birds to ascites, they were kept under cold conditions. Heritability for heart ratio was 0.43, and the heritability estimates were low: 0.02 for pvCO(2), 0.03 for pvO(2), and 0.07 for blood oxygen saturation. The estimated heritability for pH was 0.15, for bicarbonate was 0.19, and for total carbon dioxide content was 0.19. The genetic correlations between heart ratio and total carbon dioxide content (0.31 +/- 0.15) and between heart ratio and bicarbonate (0.31 +/- 0.15) were moderate and positive. For pvO(2), the genetic correlation with heart ratio was stronger and negative (-0.62 +/- 0.21); however, this correlation could not be estimated accurately because of the low heritability of pvO(2). For pvCO(2), the genetic correlation with the heart ratio was close to zero (-0.04 +/- 0.45). Phenotypic correlations between traits were, in general, similar to the genetic correlations. Heritabilities for blood gas parameters and the genetic correlations between blood gas parameters and the heart ratio estimated in the present study

  15. Female Buying Behaviour Related to Facial Skin Care Products

    OpenAIRE

    Kokoi, Isa

    2011-01-01

    This study examines the buying behaviour of Finnish women related to facial skin care products. The primary purpose of the study is to discover the similarities and differences in the buying behaviour of young and middle-aged women when purchasing facial skin care products. The objective is to study what kinds of factors affect the buying behaviour of both young (20 to 35 years old) and middle-aged (40 to 60 years old) women and then compare the findings from both groups. Furthermore, the aim...

  16. Acute and Conditioned Blood Pressure Changes in Relation to Social and Psychosocial Stimuli in Rats

    OpenAIRE

    Fokkema, Dirk S.; Koolhaas, Jaap M.

    1985-01-01

    The naturally occurring tendency to compete with other rats for territorial space has been used to study individual behavior characteristics and blood pressure reactivity to social stimuli in adult male TMD-S3 rats. The competitive characteristics of the individual rats are consistent in two different social situations (victory and defeat). Blood pressure responses during the victory of home territory rats over intruders was more pronounced in the more competitive animals. In addition to defe...

  17. Breast cancer incidence in Greek women in relation to ABO blood groups and Rh factor

    OpenAIRE

    Stamatakos, Michael; Kontzoglou, Konstantinos; Safioleas, Panagiotis; Safioleas, Constnatinos; Manti, Christina; Safioleas, Michael

    2009-01-01

    Aim To investigate the correlation between breast cancer in Greek women and ABO blood groups. Material-methods In 166 female patients with breast cancer factors such as blood group, histological type, family history, presence or absence of nodal and/or distant metastases were examined. These patients had similar demographic, clinical, surgical, immunohistochemical, laboratory, and follow-up data and this group is representative of general population of women in Greece. Results The ductal type...

  18. Time related variations in stem cell harvesting of umbilical cord blood

    OpenAIRE

    Gianluigi Mazzoccoli; Giuseppe Miscio; Andrea Fontana; Massimiliano Copetti; Massimo Francavilla; Alberto Bosi; Federico Perfetto; Alice Valoriani; Angelo De Cata; Michele Santodirocco; Angela Totaro; Rosa Rubino; Lazzaro di Mauro; Roberto Tarquini

    2016-01-01

    Umbilical cord blood (UCB) contains hematopoietic stem cells and multipotent mesenchymal cells useful for treatment in malignant/nonmalignant hematologic-immunologic diseases and regenerative medicine. Transplantation outcome is correlated with cord blood volume (CBV), number of total nucleated cells (TNC), CD34+ progenitor cells and colony forming units in UCB donations. Several studies have addressed the role of maternal/neonatal factors associated with the hematopoietic reconstruction pote...

  19. EFSA Panel on Dietetic Products, Nutrition and Allergies (NDA); Scientific Opinion on the substantiation of a health claim related to 3 g/day plant stanols as plant stanol esters and lowering blood LDL-cholesterol and reduced risk of (coronary) heart disease pursuant to Article 14 of Regulation (EC) No 1924/2006

    DEFF Research Database (Denmark)

    Tetens, Inge

    for an extended range of foods, including yellow fat spreads, dairy products, cheese, rye bread, oatmeal, fermented soy milk based products (drinkable and spoonable yoghurt-type products), and oat based milk drinks. The applicant provided an unpublished meta-analysis with 18 randomised, controlled human studies...

  20. Rapid detection of cancer related DNA nanoparticulate biomarkers and nanoparticles in whole blood

    Science.gov (United States)

    Heller, Michael J.; Krishnan, Raj; Sonnenberg, Avery

    2010-08-01

    The ability to rapidly detect cell free circulating (cfc) DNA, cfc-RNA, exosomes and other nanoparticulate disease biomarkers as well as drug delivery nanoparticles directly in blood is a major challenge for nanomedicine. We now show that microarray and new high voltage dielectrophoretic (DEP) devices can be used to rapidly isolate and detect cfc-DNA nanoparticulates and nanoparticles directly from whole blood and other high conductance samples (plasma, serum, urine, etc.). At DEP frequencies of 5kHz-10kHz both fluorescent-stained high molecular weight (hmw) DNA, cfc-DNA and fluorescent nanoparticles separate from the blood and become highly concentrated at specific DEP highfield regions over the microelectrodes, while blood cells move to the DEP low field-regions. The blood cells can then be removed by a simple fluidic wash while the DNA and nanoparticles remain highly concentrated. The hmw-DNA could be detected at a level of <260ng/ml and the nanoparticles at <9.5 x 109 particles/ml, detection levels that are well within the range for viable clinical diagnostics and drug nanoparticle monitoring. Disease specific cfc-DNA materials could also be detected directly in blood from patients with Chronic Lymphocytic Leukemia (CLL) and confirmed by PCR genotyping analysis.

  1. ABO blood groups in relation to breast carcinoma incidence and associated prognostic factors in Moroccan women.

    Science.gov (United States)

    Zouine, S; Marnissi, F; Otmani, N; Bennani Othmani, M; El Wafi, M; Kojok, K; Zaid, Y; Tahiri Jouti, N; Habti, N

    2016-07-01

    The association between blood groups ABO and different types of diseases was established in several previous studies. Our aim was to seek the possible association between the ABO blood group and breast cancer-associated prognostic factors. The Chi-squared analytic test was used to compare phenotypic ABO distribution among Moroccan blood donors and 442 cases of women suffering from breast carcinoma with archived files in Maternity Ward of University Hospital C.H.U Ibn Rochd between 2008 and 2011. High incidence of breast carcinoma was observed in blood type B patients (p type B was associated with breast carcinomas overexpressing human epidermal growth factor receptor HER2 (p risk of cancer at age over 70 years (p type A was associated with high risk of cancer among women younger than 35 years old. Blood type A and AB were associated with high incidence of lymph node metastasis (p type and estrogen receptor-positive tumor. Patients with blood group A, B, and AB were more likely to develop aggressive breast carcinoma. Further follow-up studies are necessary to clarify the role of ABH antigens in the progression of breast carcinoma. PMID:27241035

  2. Blood expression levels of chemokine receptor CCR3 and chemokine CCL11 in age-related macular degeneration

    DEFF Research Database (Denmark)

    Falk, Mads Krüger; Singh, Amardeep; Faber, Carsten; Nissen, Mogens Holst; Hviid, Thomas; Sørensen, Torben Lykke

    2014-01-01

    Dysregulation of the CCR3/CCL11 pathway has been implicated in the pathogenesis of choroidal neovascularisation, a common feature of late age-related macular degeneration (AMD). The aim of this study was to investigate the expression of CCR3 and its ligand CCL11 in peripheral blood in patients with...

  3. Head current during and blood splashes after electrical stunning in relation to characteristics of the pig's body.

    Science.gov (United States)

    Lammens, V; Van de Water, G; Coenegrachts, J; Driessen, B; Peeters, E; Geers, R

    2006-01-01

    In order to evaluate electrical stunning in relation to the incidence of blood splashes, meat quality and carcass quality parameters, 2035 pigs, heterozygous for the halothane gene, were observed during slaughter at a commercial slaughterhouse. The head current during electrical stunning is significantly higher (Pelectrical stunning can be improved by providing an energy level, based on the body characteristics of the pig. PMID:22061384

  4. Complement activation-related pseudoallergy: a stress reaction in blood triggered by nanomedicines and biologicals.

    Science.gov (United States)

    Szebeni, Janos

    2014-10-01

    Intravenous injection of a variety of nanotechnology enhanced (liposomal, micellar, polymer-conjugated) and protein-based (antibodies, enzymes) drugs can lead to hypersensitivity reactions (HSRs), also known as infusion, or anaphylactoid reactions. The molecular mechanism of mild to severe allergy symptoms may differ from case to case and is mostly not known, however, in many cases a major cause, or contributing factor is activation of the complement (C) system. The clinical relevance of C activation-related HSRs, a non-IgE-mediated pseudoallergy (CARPA), lies in its unpredictability and occasional lethal outcome. Accordingly, there is an unmet medical need to develop laboratory assays and animal models that quantitate CARPA. This review provides basic information on CARPA; a short history, issues of nomenclature, incidence, classification of reactogenic drugs and symptoms, and the mechanisms of C activation via different pathways. It is pointed out that anaphylatoxin-induced mast cell release may not entirely explain the severe reactions; a "second hit" on allergy mediating cells may also contribute. In addressing the increasing requirements for CARPA testing, the review evaluates the available assays and animal models, and proposes a possible algorithm for the screening of reactogenic drugs and hypersensitive patients. Finally, an analogy is proposed between CARPA and the classic stress reaction, suggesting that CARPA represents a "blood stress" reaction, a systemic fight of the body against harmful biological and chemical agents via the anaphylatoxin/mast-cell/circulatory system axis, in analogy to the body's fight of physical and emotional stress via the hypothalamo/pituitary/adrenal axis. In both cases the response to a broad variety of noxious effects are funneled into a uniform pattern of physiological changes. PMID:25124145

  5. Multivariate evaluation of brain function by measuring regional cerebral blood flow and event-related potentials

    Energy Technology Data Exchange (ETDEWEB)

    Koga, Yoshihiko; Mochida, Masahiko; Shutara, Yoshikazu; Nakagawa, Kazumi [Kyorin Univ., Mitaka, Tokyo (Japan). School of Medicine; Nagata, Ken

    1998-07-01

    To measure the effect of events on human cognitive function, effects of odors by measurement regional cerebral blood flow (rCBF) and P300 were evaluated during the auditory odd-ball exercise. PET showed the increase in rCBF on the right hemisphere of the brain by coffee aroma. rCBF was measured by PET in 9 of right-handed healthy adults men, and P300 was by event-related potential (ERP) in each sex of 20 right-handed healthy adults. ERP showed the difference of the P300 amplitude between men and women, and showed the tendency, by odors except the lavender oil, that women had higher in the P300 amplitude than men. These results suggest the presence of effects on the cognitive function through emotional actions. Next, the relationship between rCBF and ERP were evaluated. The subjects were 9 of the right-handed healthy adults (average: 25.6{+-}3.4 years old). rCBF by PET and P300 amplitude by ERP were simultaneously recorded during the auditory odd-ball exercise using the tone-burst method (2 kHz of the low frequency aimed stimuli and 1 kHz of the high frequency non-aimed stimuli). The rCBF value was the highest at the transverse gyrus of Heschl and the lowest at the piriform cortex among 24 regions of interest (ROI) from both sides. The difference of P300 peak latent time among ROI was almost the same. The brain waves from Cz and Pz were similar and the average amplitude was highest at Pz. We found the high correlation in the right piriform cortex (Fz), and right (Fz, Cz) and left (Cz, Pz) transverse gyrus of Heschl between the P300 amplitude and rCBF. (K.H.)

  6. Multivariate evaluation of brain function by measuring regional cerebral blood flow and event-related potentials

    International Nuclear Information System (INIS)

    To measure the effect of events on human cognitive function, effects of odors by measurement regional cerebral blood flow (rCBF) and P300 were evaluated during the auditory odd-ball exercise. PET showed the increase in rCBF on the right hemisphere of the brain by coffee aroma. rCBF was measured by PET in 9 of right-handed healthy adults men, and P300 was by event-related potential (ERP) in each sex of 20 right-handed healthy adults. ERP showed the difference of the P300 amplitude between men and women, and showed the tendency, by odors except the lavender oil, that women had higher in the P300 amplitude than men. These results suggest the presence of effects on the cognitive function through emotional actions. Next, the relationship between rCBF and ERP were evaluated. The subjects were 9 of the right-handed healthy adults (average: 25.6±3.4 years old). rCBF by PET and P300 amplitude by ERP were simultaneously recorded during the auditory odd-ball exercise using the tone-burst method (2 kHz of the low frequency aimed stimuli and 1 kHz of the high frequency non-aimed stimuli). The rCBF value was the highest at the transverse gyrus of Heschl and the lowest at the piriform cortex among 24 regions of interest (ROI) from both sides. The difference of P300 peak latent time among ROI was almost the same. The brain waves from Cz and Pz were similar and the average amplitude was highest at Pz. We found the high correlation in the right piriform cortex (Fz), and right (Fz, Cz) and left (Cz, Pz) transverse gyrus of Heschl between the P300 amplitude and rCBF. (K.H.)

  7. Hiring, Developing, and Organizing Individual Employees for New Product Development versus Product-related Service Innovation

    DEFF Research Database (Denmark)

    Knudsen, Mette Præst; Schleimer, Stephanie

    product-related service innovations, there are also important differences between these. In particular, whilst the educational level of employees has a significant and positive effect on new product development success, hiring highly educated individuals has a significant and negative effect on new...... value of human resource hiring and developing practices for new product development success; organizations will find it more beneficial to invest predominantly in employees with the highest possible educational level, whilst for product-related service innovations; employees with more general skills...... outcomes. An analysis of 335 survey responses of the multi-topic and multi-country European Manufacturing Survey (EMS) results in a number of interesting findings. First and foremost, although there are commonalities in organizing employees in flexible team arrangements for new product development versus...

  8. Strain-related acid production by oral streptococci

    DEFF Research Database (Denmark)

    de Soet, JJ; Nyvad, Bente; Kilian, Mogens

    2000-01-01

    . The bacteria were grown until mid log-phase under anaerobic conditions and acid production was measured in a pH-stat system at pH 7.0, 6.0, 5.5 and 5.0. At all pH values, the mean velocity of acid production (V(ap)) by Streptococcus mutans and S. sobrinus was significantly higher (p<0.01; ANOVA) than...... of S. mutans. The V(ap) decreased with pH for all strains, but some strains of S. mitis biovar 1 and strains of the mutans streptococci maintained a relatively high rate of acid production. The results suggest that some strains of S. mitis biovar 1 and S. oralis may play an important role in caries...

  9. On a relation between production processes and total cross sections

    CERN Document Server

    Munier, Stephane

    2013-01-01

    Perturbative QCD is the appropriate tool to describe many important properties of the inclusive observables measured at electron-proton (or ion) colliders, such as the energy dependence of the total cross sections in well-chosen kinematical regions. This is because the electron may effectively be replaced by its cloud of photons, whose virtualities provide a hard scale that enables perturbative expansions. At hadron colliders instead, there is no hard scale in the initial state. Therefore, the observables one may compute perturbatively involve the production of jets, and thus belong to a quite different class of observables. However, it turns out that there is a formal relation between production processes and total cross sections, enabling one to apply calculations of the latter to the former. We review this relation, and present our recent proof that it holds at next-to-leading order (in the BFKL sense).

  10. Relation of electrocardiographic left ventricular hypertrophy to blood pressure, body mass index, serum lipids and blood sugar levels in adult Nigerians.

    Science.gov (United States)

    Opadijo, O G; Omotoso, A B O; Akande, A A

    2003-12-01

    Left ventricular hypertrophy (LVH) is considered an independent risk factor even in the absence of systemic hypertension. Electrocardiographic (ECG) LVH with repolarisation changes has been found in some countries to carry more coronary risk than LVH alone. How far this observation is true among adult Nigerians is not known. We therefore decided to study adult Nigerians with ECG-LVH with or without ST-T waves changes and compare them with normal age matched controls (without ECG-LVH) in relation with established modifiable risk factors such as systemic hypertension (BP), body mass index (BMI), fasting blood sugar (FBS) and serum lipids such as total cholesterol (Tc), low density lipoprotein cholesterol (LDL-C), high density lipoprotein cholesterol (HDL-C) and triglyceride (TG). Adult Nigerians who were consecutively referred to the ECG laboratory were randomly recruited. Three hundred patients were studied. Their blood pressures (BP) as well as body mass indices were recorded after recording their resting 12 read ECG using portable Seward 9953 ECG machine. Their waist-hip ratio (WHR) was also recorded. Blood samples were taken to determine their fasting blood sugar and serum lipids. Their ECG tracings were read by the cardiologists involved in the study while the blood samples were analysed by the chemical pathologist also involved in the study. At the end of the ECG reading, the patients were divided into 3 groups according to whether there was no ECG-LVH (control group A), ECG-LVH alone (group B), and ECG-LVH with ST-T waves changes (group C). One hundred and fifty (50%) patients belonged to group A, 100 (33.3%) patients to group B and 50 (16.7%) group C. Group B patients were found to have higher modifiable risk factors in form of systemic BP. Tc, LDL-C, and WHR compared to group A. However, the group C patients had increased load of these coronary risk factors in terms of BP elevation, higher BMI, FBS, and scrum cholesterol compared to group B. In addition

  11. Post Mortem Blood and Vitreous Humor Ethanol Levels Relation in Fars Legal Medicine Center

    Directory of Open Access Journals (Sweden)

    Aria Hejazi

    2012-11-01

    Full Text Available Background and Objective: The concentration of ethanol measured in postmortem blood needs to be interpreted as to whether the person who had consumed alcohol has exceeded some threshold limit. However, interpreting postmortem BAC (Blood Alcohol Concentration results and drawing correct conclusions about ante mortem levels is fraught with difficulties as under some circumstances alcohol might be produced after death by microbial activity.Subjects and Methods: In the case of ethanol, the blood samples should be taken from a femoral vein and whenever possible additional specimens, such as urine and vitreous humor (VH, should also be obtained and sent for analysis to be able to have the best interpretationResults: 167 Specimens of femoral vein blood and vitreous humor were analyzed. In this study, direct injection and gas-chromatographic techniques were employed to quantitate the ethanol concentrations. These two concentrations were then compared and the BAC/VHAC (Viterous Humor Alcohol Concentration ratio was evaluated.Among these specimens, there were 142 cases in which we have found ethanol in blood but not in vitreous humor. Also we have found 2 cases by positive VHAC but negative BAC. The average blood ethanol concentration was 61.5 mg/dLit while the average ethanol vitreous humor concentration was 127 mg/dLit. However, the ratio was measured as 0.7.Conclusion: The similarity in the results of the post mortem cases reinforces the assertion that co analysis of vitreous humor and blood is important to interpret post mortem results

  12. Nuclear and related techniques in animal production and health

    International Nuclear Information System (INIS)

    The international symposium was attended by about 130 participants from 45 countries and included 83 scientific presentations of which 42 were posters. This volume covers four principal and interrelated topics: adaptation of animals to the environment, and animal reproduction, health and nutrition. Within each topic, consideration is given to those nuclear and related techniques currently employed in investigative research and their usefulness in studying animal production systems. Progress towards new areas of application and new techniques is also covered, particularly the development and practicability of immunoassay and related biotechnological methods for the diagnosis of livestock diseases. A separate abstract was prepared for each of the papers in this volume

  13. Exclusive Pion-production and the Relation to Transversity

    CERN Document Server

    Goldstein, Gary R

    2008-01-01

    Exclusive $\\pi^0$ electroproduction from nucleons is suggested for extracting the tensor charge and other quantities related to transversity from experimental data. This process isolates C-parity odd and chiral odd combinations of t-channel exchange quantum numbers. In a hadronic picture it connects the meson production amplitudes to C-odd Regge exchanges with final state interactions. In a description based on partonic degrees of freedom, the helicity structure for this C-odd process relates to the quark helicity flip, or chiral odd generalized parton distributions.

  14. Radiographic evaluation of destructive periodontal disease in blue mink in relation to age and blood morphology

    DEFF Research Database (Denmark)

    Hammer, Anne Sofie; Andersen, Thomas Holmen; Eriksen, Thomas;

    2005-01-01

    bone loss and tooth loss. There was a high prevalence of destructive periodontal disease among blue mink included in this study. Mild to moderate periodontal disease (defined by less than 50% alveolar bone loss related to 1 or more teeth) affected 73.7% of young mink (age = 7 mo) and 67.9% of older...... animals (age &GE; 19 mo). Severe periodontal disease (defined by more than 50% bone loss related to one or more teeth) was not detected in mink aged 7 mo, but affected 15.3% of mink aged 19 mo and 39.6% of mink aged 31 mo. The positive relationship between age and periodontal disease was statistically...... in the mink was related to and possibly caused by destructive periodontal disease. There was no significant difference in the prevalence of periodontal disease between the 2 genotypes and age was found to be the only statistical predictor of poor production results (P < 0.01) in blue mink....

  15. Radiographic evaluation of destructive periodontal disease in blue mink in relation to age and blood morphology

    DEFF Research Database (Denmark)

    Hammer, Anne Sofie; Andersen, Thomas Holmen; Eriksen, Thomas; Kortegaard, Hanne E.; Dietz, Hans Henrik; Chriél, Mariann

    2005-01-01

    animals (age &GE; 19 mo). Severe periodontal disease (defined by more than 50% bone loss related to one or more teeth) was not detected in mink aged 7 mo, but affected 15.3% of mink aged 19 mo and 39.6% of mink aged 31 mo. The positive relationship between age and periodontal disease was statistically...... bone loss and tooth loss. There was a high prevalence of destructive periodontal disease among blue mink included in this study. Mild to moderate periodontal disease (defined by less than 50% alveolar bone loss related to 1 or more teeth) affected 73.7% of young mink (age = 7 mo) and 67.9% of older...... in the mink was related to and possibly caused by destructive periodontal disease. There was no significant difference in the prevalence of periodontal disease between the 2 genotypes and age was found to be the only statistical predictor of poor production results (P < 0.01) in blue mink....

  16. Proportion of drug-related serious rare blood dyscrasias: estimates from the Berlin Case-Control Surveillance Study.

    Science.gov (United States)

    Andersohn, Frank; Bronder, Elisabeth; Klimpel, Andreas; Garbe, Edeltraut

    2004-11-01

    Drugs are an important cause of serious rare blood dyscrasias. To estimate the proportion of drug-related cases, we used data from the ongoing Berlin Case-Control Surveillance Study. The analysis included a total of 171 cases. The number of cases in which a drug etiology was assessed as at least "possible" was n = 29 (97%) for acute agranulocytosis, n = 4 (0.21%) for aplastic anemia, n = 8 (26%) for immune hemolytic anemia, n = 20 (25%) for immune thrombocytopenia, and n = 2 (20%) for thrombotic thrombocytopenic purpura/hemolytic uremic syndrome. Our analysis suggests that a substantial fraction of blood dyscrasias may be attributable to drug therapy. PMID:15495238

  17. Relative pressure estimation from velocity measurements in blood flows: state-of-the-art and new approaches

    OpenAIRE

    Bertoglio, Cristobal; Núñez, Rodolfo; Galarce, Felipe; Nordsletten, David; Osses, Axel

    2016-01-01

    The pressure gradient across stenotic blood vessels is an important clinical index for diagnosis of the pathological severity of the cardiovascular disease. While the clinical gold standard for its measurement is invasive catheterization, Phase-Contrast MR-imaging has emerged as a promising tool for enabling a non-invasive quantification of the relative pressures, by linking the (highly spatially resolved) velocity measurements with the relative pressure via the Navier-Stokes equations. In th...

  18. Blood phagocyte free radical production and plasma cytokine levels in patients undergoing open heart surgery or heart transplantation

    Czech Academy of Sciences Publication Activity Database

    Kubala, Lukáš; Číž, Milan; Čížová, Hana; Černý, J.; Němec, P.; Studeník, P.; Lojek, Antonín

    Antalya , 2001, s. 79. [Free Radicals, Nitric Oxide, and Inflammation: Molecular, Biochemical, and Clinical Aspects. Antalya (TR), 23.09.2001-03.10.2001] Institutional research plan: CEZ:AV0Z5004920 Keywords : blood phagocyte * free radical production * heart surgery Subject RIV: BO - Biophysics

  19. SNP may modify the effect of vitamin A supplementation at birth on cytokine production in a whole blood culture assay

    DEFF Research Database (Denmark)

    Jørgensen, Mathias Jul; Fisker, Ane Bærent; Erikstrup, Christian;

    2012-01-01

    Within a neonatal vitamin A supplementation (VAS) trial, we investigated the effect of VAS on TNF-a, IL-10, IL-5 and IL-13 production after lipopolysaccharide, purified protein derivative (PPD) of Mycobacterium tuberculosis and phytohaemagglutinin stimulation using a whole blood culture protocol....

  20. Dynamic Infrared Thermography Study of Blood Flow Relative to Lower Limp Position

    Science.gov (United States)

    Stathopoulos, I.; Skouroliakou, K.; Michail, C.; Valais, I.

    2015-09-01

    Thermography is an established method for studying skin temperature distribution. Temperature distribution on body surface is influenced by a variety of physiological mechanisms and has been proven a reliable indicator of various physiological disorders. Blood flow is an important factor that influences body heat diffusion and skin temperature. In an attempt to validate and further elucidate thermal models characterizing the human skin, dynamic thermography of the lower limp in horizontal and vertical position was performed, using a FLIR T460 thermographic camera. Temporal variation of temperature was recorded on five distinct points of the limp. Specific points were initially cooled by the means of an ice cube and measurements of the skin temperature were obtained every 30 seconds as the skin temperature was locally reduced and afterwards restored at its initial value. The return to thermal balance followed roughly the same pattern for all points of measurement, although the heating rate was faster when the foot was in horizontal position. Thermal balance was achieved faster at the spots that were positioned on a vein passage. Our results confirm the influence of blood flow on the thermal regulation of the skin. Spots located over veins exhibit different thermal behaviour due to thermal convection through blood flow. Changing the position of the foot from vertical to horizontal, effectively affects blood perfusion as in the vertical position blood circulation is opposed by gravity.

  1. The evaluation of iron deficiency and anemia in male blood donors with other related factors

    Directory of Open Access Journals (Sweden)

    Yousefinejad Vahid

    2010-01-01

    Full Text Available Aims and Background: Iron deficiency is one of the most common nutritional disorders worldwide and blood donation may cause iron depletion. Limited studies with large sample size have been done on male donors. The aim of this study is to determine the prevalence of iron deficiency and iron deficiency anemia among male donors in the Kurdistan Organization of Blood Transfusion in Iran. Materials and Methods: This was a cross-sectional study. Sample size was 1184 blood donors selected by systematic random sampling. Hemoglobin, serum iron, serum ferritin, total iron banding capacity (TIBC and transferin saturation were measured in donors. Iron depletion, lack of iron stores, iron deficiency, iron deficiency anemia and anemia were evaluated among them. Data was analyzed with SPSS software and X΂, one-way ANOVA, and LSD test. Results: Iron deficiency, anemia, iron deficiency anemia, iron depletion and lack of iron resources were seen in 2.3, 4.08, 2.14, 22.76 and 4.66 percent respectively. There was a significant relationship of iron deficiency and iron deficiency anemia with instances of donation and interval from last donation (P < 0.05. A significant relationship was seen between iron deficiency and iron deficiency anemia among blood donors with more than ten times blood donation (P < 0.05. Conclusions: This study showed regular male donors require especial attention. Therefore, serum ferritin is recommended as a more adequate index to use for iron deficiency screening and planning purposes for iron supplementation among them.

  2. Occupational lead poisoning in the United States: Clinical and biochemical findings related to blood lead levels

    Energy Technology Data Exchange (ETDEWEB)

    Baker, E.L.; Landrigan, P.J.; Barbour, A.G.; Cox, D.H.; Folland, D.S.

    1977-01-01

    A study was made of 160 lead exposed workers at a secondary lead smelter, a small scrap smelter, and a lead chemicals facility to investigate dose response relationships between blood lead levels and toxic effects. The levels of blood lead ranged from 0.77 to 13.51 micromoles/liter (micromol/l). In 70 workers, 44 percent of the total number, clinical evidence of toxic exposure was detected including colic in 33, wrist or ankle extensor muscle weakness in 12, anemia in 27, elevated blood urea nitrogen (BUN) in 28, and possible encephalopathy in two. At blood lead levels below 1.93 micromol/l no toxicity was detected. However, 13 percent of those workers with blood lead levels of 1.93 to 3.81 micromol/l had extensor muscle weakness or gastrointestinal symptoms. In 5 percent of the workers with lead levels of 1.93 to 2.85 micromol/l, anemia was noted. Anemia was also noted in 14 percent with levels between 2.90 and 3.81 micromol/l and in 36 percent with levels over 3.86 micromol/l. In long term lead workers elevated BUN occurred. All but three workers with elevated BUN had at least 4 years of occupational lead exposure, and nine had received oral chelation therapy. Eight of this group had reduced creatinine clearance and eight had decreased renal concentrating ability.

  3. Are anemia and blood group types related to Japanese encephalitis and dengue?

    Directory of Open Access Journals (Sweden)

    Shantanu Sharma

    2015-01-01

    Full Text Available Introduction: Dengue and Japanese encephalitis (JE are flaviviruses causing significant morbidity and mortality worldwide. Previous studies had found an association between dengue infection and particular blood group antigen and also the susceptibility to aplastic anemia by dengue. Aims and Objective: The present study was conducted to find any association between dengue and JE immunoglobulin G (IgG antibody with blood group antigen or anemia. Methodology: The study was conducted in 2 slums of Delhi in 2013 in the interepidemic period. 5 mL blood from 239 samples were taken and processed for IgG levels of dengue and JE and rest used to test hemoglobin and blood group. Results and Discussion: No association has been found between blood group types and dengue IgG antibody positivity (P = 0.42 or JE IgG antibody positivity (P = 0.148. Nor did anemia established any association with dengue (P = 0.185 or JE IgG antibody (P = 0.277. IgG status of dengue and JE could be used as proxy markers of any past subclinical infection (includes clinically manifested cases also. Further studies in large settings and other areas could be done to validate the results. This was the first study of its kind in such settings.

  4. Blood irradiation with accelerator produced electron beams

    International Nuclear Information System (INIS)

    Blood and blood products are irradiated with gamma rays to reduce the risk of graft versus host disease (GVHD). A simple technique using electron beams produced by a medical linear accelerator has been studied to evaluate irradiation of blood and blood products. Variations in applied doses for a single field 20 MeV electron beam are measured in a phantom study. Doses have been verified with ionization chambers and commercial diode detectors. Results show that the blood product volume can be given a relatively homogeneous dose to within 6% using 20 MeV electrons without the need to rotate the blood bags or the beam entry point. The irradiation process takes approximately 6.5 minutes for 30 Gy applied dose to complete as opposed to 12 minutes for a dual field x-ray field irradiation at our centre. Electron beams can be used to satisfactorily irradiate blood and blood products in a minimal amount of time. (author)

  5. Effect of live yeast culture Saccharomyces cerevisiae on milk production and some blood parameters

    Directory of Open Access Journals (Sweden)

    Judit Peter Szucs

    2013-05-01

    Full Text Available The aim of this study was to investigate the effect of live yeast culture (Saccharomyces cerevisiae Sc 47 on milk yield, milk composition and some blood parameters of dairy cows during their early lactation on farm conditions. The live yeast culture was given in the diet of heifers and cows (5 g day-1 solid Actisaf for 14 days before calving and exclusively for the treated cows 12 g day-1 dissolved in 500 ml of water, during 14 days after calving. The experiment took until 100th day of lactation on farm conditions. Yeast culture supplementation was the most effective for the performance of primiparous cows: It was advantageous for blod plasma parameters: decreased the beta-hydroxy butyrate (BHB content and free fatty acids (FFA which indicated the protection of the animals against ketosis or other metabolic disorders. Increased the daily milk production and the lactose /glucose content of the milk. The live yeast culture increased the lactose content of the milk and decreased the somatic cell count of multiparous cows. The listed parameters were not significant (P<0.05 compare to the results of positive control groups. The applied live yeast culture supplementation did not significant affect for other performance of the cows.

  6. Controlled meal frequency without caloric restriction alters peripheral blood mononuclear cell cytokine production

    Directory of Open Access Journals (Sweden)

    Longo Dan L

    2011-03-01

    Full Text Available Abstract Background Intermittent fasting (IF improves healthy lifespan in animals by a mechanism involving reduced oxidative damage and increased resistance to stress. However, no studies have evaluated the impact of controlled meal frequency on immune responses in human subjects. Objective A study was conducted to establish the effects of controlled diets with different meal frequencies, but similar daily energy intakes, on cytokine production in healthy male and female subjects. Design In a crossover study design with an intervening washout period, healthy normal weight middle-age male and female subjects (n = 15 were maintained for 2 months on controlled on-site one meal per day (OMD or three meals per day (TMD isocaloric diets. Serum samples and peripheral blood mononuclear cells (PBMCs culture supernatants from subjects were analyzed for the presence of inflammatory markers using a multiplex assay. Results There were no significant differences in the inflammatory markers in the serum of subjects on the OMD or TMD diets. There was an increase in the capacity of PBMCs to produce cytokines in subjects during the first month on the OMD or TMD diets. Lower levels of TNF-α, IL-17, MCP-1 and MIP-1β were produced by PBMCs from subjects on the OMD versus TMD diet. Conclusions PBMCs of subjects on controlled diets exhibit hypersensitivities to cellular stimulation suggesting that stress associated with altered eating behavior might affect cytokine production by immune cells upon stimulation. Moreover, stimulated PBMCs derived from healthy individuals on a reduced meal frequency diet respond with a reduced capability to produce cytokines.

  7. Circulating thrombopoietin levels in normal healthy blood donors and in aplastic anemia patients in relation to disease severity

    OpenAIRE

    2015-01-01

    Background: Thrombopoietin (TPO) is the key hematopoietic growth factor regulating the production of platelets from bone marrow megakaryocytes and maintaining platelet hemostasis. This study was done to find any relationship between the levels of thrombopoietin and the severity of disease in patients with aplastic anemia. Materials and Methods: Serum samples were collected from 52 patients with a confirmed diagnosis of aplastic anemia and 45 normal healthy blood donors of both sexes over a pe...

  8. The Relation of Body Mass Index and Blood Pressure in Iranian Children and Adolescents Aged 7- 18 Years Old

    Directory of Open Access Journals (Sweden)

    M Hosseini

    2010-12-01

    Full Text Available Background: The obesity and hypertension are the major risk factors of several life threatening diseases. The present study was aimed to investigate the relation between body mass index (BMI the validated index of adiposity and different aspect of blood pressure (BP. Methods: Systolic and diastolic blood pressures and also weight and height of 7 to 18 years old children and adolescent col­lected in 2002 and 2004 respectively. Data was consisted of 14865 schoolchildren and adolescents from representative sam­ple of country. BMI was classified according to CDC 2000 standards into normal (BMI<85th percentile, at risk of over­weight (BMI≥85th and <95th percentile and overweight (BMI≥ 95th percentile. Then, age-sex specific prevalence of be­ing overweight was derived. ANOVA was used to investigate the effect of BMI on systolic blood pressure and diastolic blood pressure and mean arterial pressure of participants. Results: Mean systolic blood pressure (SBP, diastolic blood pressure (DBP and mean arterial blood pressure (MAP signifi­cantly increased with BMI (P< 0.0001 and age groups (P< 0.0001, and was significantly (P< 0.0001 higher in boys than girls especially in older ages. (P< 0.0001, interaction of age and BMI level. The proportion of being overweight was signifi­cantly higher in boys than girls was (7.4% vs. 3.6%; P< 0.0001. Conclusion: There is an association between BP and BMI in children and adolescence. SBP, DBP and MAP are associated with rise in BMI and age, which was lower in girls. This data can provide basics for public health policy makers and pri­mary prevention policies in the country.

  9. Blood lead level in dogs from urban and rural areas of India and its relation to animal and environmental variables

    International Nuclear Information System (INIS)

    Lead is a common environmental pollutant with deleterious health effects on human and animal. Industrial and other human activities enhance the lead level in the environment leading to its higher residues in exposed population. The present study was aimed at determining blood lead concentration in dogs from two urban areas and in surrounding rural areas of India and analyzing lead level in dogs in relation to environmental (urban/ rural) and animal (age, sex, breed and housing) variables. Blood samples were collected from 305 dogs of either sex from urban (n = 277) and unpolluted rural localities (n = 28). Irrespective of breed, age and sex, the urban dogs had significantly (P < 0.01) higher mean blood lead concentration (0.25 ± 0.01 μg/ml) than rural dogs (0.10 ± 0.01 μg/ml). The mean blood lead level in stray dogs either from urban or rural locality (0.27 ± 0.01 μg/ml) was significantly (P < 0.01) higher than that of pets (0.20 ± 0.01 μg/ml), and the blood lead concentration was significantly higher in nondescript dogs (0.25 ± 0.01 μg/ml) than pedigreed dogs (0.20 ± 0.01 μg/ml). The locality (urban/rural) was the major variable affecting blood lead concentration in dogs. Breed and housing of the dogs of urban areas and only housing (pet/stray) in rural areas significantly (P < 0.01) influenced the blood lead concentration in dogs

  10. Asthma-related productivity losses in Alberta, Canada

    Directory of Open Access Journals (Sweden)

    Nguyen X Thanh

    2009-03-01

    Full Text Available Nguyen X Thanh, Arto Ohinmaa, Charles YanInstitute of Health Economics, Edmonton, Alberta, CanadaObjectives: To estimate the number and cost of asthma-related productivity loss days due to absenteeism and presenteeism (at work but not fully functioning in Alberta in 2005.Methods: Using data from the 2005 Canadian Community Health Survey, this study focused on people of working age (18–64 years, who reported having an asthma diagnosis. Total asthma-related disability days, including in-bed days and activity-restricted days, were estimated by multiplying the difference in the means of total disability days between asthmatics and nonasthmatics adjusted for sociodemographic characteristics and other health conditions by a multiple linear regression, with the number of asthmatics in the population. Number of productivity loss days was a sum between the number of in-bed days (absenteeism and the number of activity-restricted days multiplied by a reduction in functional level (presenteeism, adjusted for five working days per week. Other data from Alberta or Canadian published literature, such as a reduction in functional level of 20%–30%, a labor participation rate of 73%, and an average wage of $158 per day in 2005, were also used for analyses.Results: The prevalence of asthma was estimated at 8.5% among approximately 2.1 million people of working age in Alberta in 2005. The difference in the means of total disability days between asthmatics and nonasthmatics was 0.487 (95% CI: 0.286–0.688 in a period of two weeks or 12.7 (7.5–17.9 in one year. With the reduction in functional level of 20%–30%, the number of asthma-related productivity loss days was estimated from 442 (259–624 to 533 (313–753 thousand, respectively. The corresponding cost was from $70 ($41–$99 to $84 ($49–$119 million. Of these, the presenteeism accounted for 42% to 52%.Conclusions: The results suggest that an improvement in the controlling of asthma could have a

  11. Diving Related Changes in the Blood Oxygen Stores of Rehabilitating Harbor Seal Pups (Phoca vitulina.

    Directory of Open Access Journals (Sweden)

    Amber Thomas

    Full Text Available Harbor seal (Phoca vitulina pups begin diving within hours of birth, stimulating the development of the blood oxygen (O2 stores necessary to sustain underwater aerobic metabolism. Since harbor seals experience a brief nursing period, the early-life development of these blood O2 stores is necessary for successful post-weaning foraging. If mothers and pups become prematurely separated, the pup may be transported to a wildlife rehabilitation center for care. Previous studies suggest that the shallow pools and lack of diving in rehabilitation facilities may lead to under-developed blood O2 stores, but diving behavior during rehabilitation has not been investigated. This study aimed to simultaneously study the diving behaviors and blood O2 store development of rehabilitating harbor seal pups. Standard hematology measurements (Hct, Hb, RBC, MCV, MCH, MCHC were taken to investigate O2 storage capacity and pups were equipped with time-depth recorders to investigate natural diving behavior while in rehabilitation. Linear mixed models of the data indicate that all measured blood parameters changed with age; however, when compared to literature values for wild harbor seal pups, rehabilitating pups have smaller red blood cells (RBCs that can store less hemoglobin (Hb and subsequently, less O2, potentially limiting their diving capabilities. Wild pups completed longer dives at younger ages (maximum reported <25 days of age: 9 min in previous studies than the captive pups in this study (maximum <25 days of age: 2.86 min. However, captivity may only affect the rate of development, as long duration dives were observed (maximum during rehabilitation: 13.6 min at 89 days of age. Further, this study suggests that there may be a positive relationship between RBC size and the frequency of long duration dives. Thus, rehabilitating harbor seal pups should be encouraged to make frequent, long duration dives to prepare themselves for post-release foraging.

  12. Retrospective evaluation of adverse transfusion reactions following blood product transfusion from a tertiary care hospital: A preliminary step towards hemovigilance

    Directory of Open Access Journals (Sweden)

    Praveen Kumar

    2013-01-01

    Full Text Available Background: The goal of hemovigilance is to increase the safety and quality of blood transfusion. Identification of the adverse reactions will help in taking appropriate steps to reduce their incidence and make blood transfusion process as safe as possible. Aims : To determine the frequency and type of transfusion reactions (TRs occurring in patients, reported to the blood bank at our institute. Materials and Methods : A retrospective review of all TRs reported to the blood bank at the All India Institute of Medical Sciences, between December 2007 and April 2012 was done. All the TRs were evaluated in the blood bank and classified using standard definitions. Results: During the study period a total of 380,658 bloods and blood components were issued by our blood bank. Out of the total 196 adverse reactions reported under the hemovigilance system, the most common type of reaction observed was allergic 55.1% (n = 108, followed by febrile non-hemolytic transfusion reaction (FNHTR 35.7% (n = 70. Other less frequently observed reactions were Anaphylactoid reactions 5.1% (n = 10, Acute non-immune HTRs 2.6% (n = 5, Circulatory overload 0.5% (n = 1, Transfusion related acute lung injury 0.5% (n = 1, Delayed HTRs 0.5% (n = 1. Not a single case of bacterial contamination was observed. Conclusion: The frequency of TRs in our patients was found to be 0.05% (196 out of 380,658. This can be an underestimation of the true incidence because of under reporting. It should be the responsibility of the blood transfusion consultant to create awareness amongst their clinical counterpart about safe transfusion practices so that proper hemovigilance system can be achieved to provide better patient care.

  13. Mercury in human brain, blood, muscle and toenails in relation to exposure: an autopsy study

    Directory of Open Access Journals (Sweden)

    Morild Inge

    2007-10-01

    Full Text Available Abstract Background The main forms of mercury (Hg exposure in the general population are methylmercury (MeHg from seafood, inorganic mercury (I-Hg from food, and mercury vapor (Hg0 from dental amalgam restorations. While the distribution of MeHg in the body is described by a one compartment model, the distribution of I-Hg after exposure to elemental mercury is more complex, and there is no biomarker for I-Hg in the brain. The aim of this study was to elucidate the relationships between on the one hand MeHg and I-Hg in human brain and other tissues, including blood, and on the other Hg exposure via dental amalgam in a fish-eating population. In addition, the use of blood and toenails as biological indicator media for inorganic and organic mercury (MeHg in the tissues was evaluated. Methods Samples of blood, brain (occipital lobe cortex, pituitary, thyroid, abdominal muscle and toenails were collected at autopsy of 30 deceased individuals, age from 47 to 91 years of age. Concentrations of total-Hg and I-Hg in blood and brain cortex were determined by cold vapor atomic fluorescence spectrometry and total-Hg in other tissues by sector field inductively coupled plasma-mass spectrometry (ICP-SFMS. Results The median concentrations of MeHg (total-Hg minus I-Hg and I-Hg in blood were 2.2 and 1.0 μg/L, and in occipital lobe cortex 4 and 5 μg/kg, respectively. There was a significant correlation between MeHg in blood and occipital cortex. Also, total-Hg in toenails correlated with MeHg in both blood and occipital lobe. I-Hg in both blood and occipital cortex, as well as total-Hg in pituitary and thyroid were strongly associated with the number of dental amalgam surfaces at the time of death. Conclusion In a fish-eating population, intake of MeHg via the diet has a marked impact on the MeHg concentration in the brain, while exposure to dental amalgam restorations increases the I-Hg concentrations in the brain. Discrimination between mercury species is

  14. Blood Sugar Values and Intestine Mucosa Integrity in Suckling Pigs in Relation to their Nutritional Status

    OpenAIRE

    Olga Rada; Horea Sărăndan; Liliana Vasile; Diana Argherie

    2010-01-01

    The experiment included 106 healthy piglets aged 1-14 days and 38 piglets suffering from diarrhoea, aged 1-7 days. The blood sugar was measured with the help of Accu-chek Go (Roche Diagnostics GMBH, Germany) halfway between two sucklings. Segments with duodenum, jejunum and ileum were harvested from the diarrhoea suffering piglets and coloured HE preparations were examined under a stereoscopic magnifying glass and the microscope. With healthy piglets, the average blood sugar was of 61.55±10.2...

  15. Relationship between office and home blood pressure with increasing age: The International Database of HOme blood pressure in relation to Cardiovascular Outcome (IDHOCO).

    Science.gov (United States)

    Ntineri, Angeliki; Stergiou, George S; Thijs, Lutgarde; Asayama, Kei; Boggia, José; Boubouchairopoulou, Nadia; Hozawa, Atsushi; Imai, Yutaka; Johansson, Jouni K; Jula, Antti M; Kollias, Anastasios; Luzardo, Leonella; Niiranen, Teemu J; Nomura, Kyoko; Ohkubo, Takayoshi; Tsuji, Ichiro; Tzourio, Christophe; Wei, Fang-Fei; Staessen, Jan A

    2016-08-01

    Home blood pressure (HBP) measurements are known to be lower than conventional office blood pressure (OBP) measurements. However, this difference might not be consistent across the entire age range and has not been adequately investigated. We assessed the relationship between OBP and HBP with increasing age using the International Database of HOme blood pressure in relation to Cardiovascular Outcome (IDHOCO). OBP, HBP and their difference were assessed across different decades of age. A total of 5689 untreated subjects aged 18-97 years, who had at least two OBP and HBP measurements, were included. Systolic OBP and HBP increased across older age categories (from 112 to 142 mm Hg and from 109 to 136 mm Hg, respectively), with OBP being higher than HBP by ∼7 mm Hg in subjects aged >30 years and lesser in younger subjects (P=0.001). Both diastolic OBP and HBP increased until the age of ∼50 years (from 71 to 79 mm Hg and from 66 to 76 mm Hg, respectively), with OBP being consistently higher than HBP and a trend toward a decreased OBP-HBP difference with aging (PDeterminants of a larger OBP-HBP difference were younger age, sustained hypertension, nonsmoking and negative cardiovascular disease history. These data suggest that in the general adult population, HBP is consistently lower than OBP across all the decades, but their difference might vary between age groups. Further research is needed to confirm these findings in younger and older subjects and in hypertensive individuals. PMID:27053011

  16. Technology diffusion of energy-related products in residential markets

    Energy Technology Data Exchange (ETDEWEB)

    Davis, L.J.; Bruneau, C.L.

    1987-05-01

    Acceptance of energy-related technologies by end residential consumers, manufacturers of energy-related products, and other influential intermediate markets such as builders will influence the potential for market penetration of innovative energy-related technologies developed by the Department of Energy, Office of Building and Community Systems (OBCS). In this report, Pacific Northwest Laboratory reviewed the available information on technology adoption, diffusion, and decision-making processes to provide OBCS with a background and understanding of the type of research that has previously been conducted on this topic. Insight was gained as to the potential decision-making criteria and motivating factors that influence the decision-maker(s) selection of new technologies, and some of the barriers to technology adoption faced by potential markets for OBCS technologies.

  17. Cumulative semantic interference for associative relations in language production.

    Science.gov (United States)

    Rose, Sebastian Benjamin; Abdel Rahman, Rasha

    2016-07-01

    Associations between conceptual representations and thematic relations play an important role in the organization of semantic memory. However, language production research on semantic context effects shows that associative (e.g., dog and bone) and categorical relations (dog and horse) seem to diverge. While categorical contexts typically induce semantic interference that has traditionally been taken to reflect competitive lexical selection, evidence for comparable associative modulations is rare. In three experiments we tested whether thematic associations between objects induce cumulative interference in the continuous naming paradigm, assuming that this paradigm hampers lexical selection via the activation of highly active lexical cohorts steadily increasing in size. Indeed, naming times increased linearly with each newly named member of thematic contexts irrespective of the pre-activation of associations before the naming task (Experiment 1), and irrespective of whether categorical links were partially included (Experiments 1 and 2) or entirely absent (Experiment 3). These findings demonstrate that different types of semantic relations induce interference. PMID:27015349

  18. Changes of metabolic syndrome related indexes before and after blood donation in blood donors%献血前后代谢综合征相关血液指标变化

    Institute of Scientific and Technical Information of China (English)

    蔡珍; 罗琼

    2015-01-01

    Objective To investigate the significance of blood donation in preventing metabolic syndrome (MS)by analyzing the changes of the blood pressure and the related biochemical indexes before and after blood donation in the blood donors with high risk of MS.Methods The blood specimens of 102 blood donors,aged 45 -55 years old with the systolic blood pressure of 110 -140 mmHg and the diastolic blood pressure of 75 -90 mmHg,the body mass index> 24 kg/m2 ,were selected as the subjects.The blood pressure,heart rate,iron reserves,blood glucose,blood lipids,and the other indicators were determined before blood donation. All the above indicators were tested again on 42 d after blood donation.Results The blood pressure,iron reserves,blood glucose and LDL/HDL ratio after donation showed significant changes compared with before blood donation(P <0.05 ).Conclusion The changes of the MS related blood indexes exist before and after blood donation,blood donation has a certain significance for preven-ting MS in the blood donors with high risk of MS.%目的:分析代谢综合征(MS)的高危献血者献血前后的血压及相关生化指标来探讨献血对预防 MS 的意义。方法研究对象为男性或绝经后女性共102例,年龄45~55岁,收缩压110~140 mm Hg,舒张压75~90 mm Hg,体质量指数大于24 kg/m2,献血前测定血压、心率、铁储量、血糖、血脂等指标与献血42 d 后再测定相同指标进行比较。结果血压、铁储量、血糖、低密度脂蛋白/高密度脂蛋白(LDL/HDL)与献血前比较差异有统计学意义(P <0.05)。结论献血前后 MS 相关血液指标存在变化,献血对于 MS 高危者预防 MS 具有一定意义。

  19. Evaluating Product-Related Hazards At the Consumer Product Safety Commission

    OpenAIRE

    Gregory B. Rodgers

    1990-01-01

    Multivariate qualitative response models, such as logit regression models, are useful in evaluating product-related risk at the Consumer Product Safety Commission (CPSC). They are used to determine and quantify the factors associated with accidents, and can suggest or justify appropriate regulatory responses. This article applies the method to evaluating risks associated with all-terrain vehicles. A logit regression model is used to analyze data from surveys of users and accident victims. Bot...

  20. Gamma-interferon bioassay for detection of bovine tuberculosis in cattle: kinetics of production and dose response in whole blood culture

    International Nuclear Information System (INIS)

    Stimulation with mycobacterium bovis PPD sensitised lymphocytes (whole blood or peripheral blood lymphocytes) results in release of gamma-interferon that can be detected by simple bioassay. The optimum concentration of bovine PPD was 20 μg ml and the optimum incubation period was 24 hr for maximum production of gamma-interferon in whole blood culture (128 units/ml) and peripheral blood culture (64 units/ml). (author)

  1. Serum selenium concentration in maternal and umbilical cord blood. Relation to course and outcome of pregnancy

    DEFF Research Database (Denmark)

    Bro, S; Berendtsen, H; Nørgaard, J; Høst, A; Jørgensen, P J

    1988-01-01

    The present knowledge of the role of selenium in human fetal and neonatal development is sparse. In this study we measured serum selenium concentrations in maternal and umbilical cord blood from 500 Danish mothers at delivery, looking for a relationship between various maternal and fetal complica...

  2. Blood-group-related carbohydrates are expressed in organotypic cultures of human skin and oral mucosa

    DEFF Research Database (Denmark)

    Grøn, B; Andersson, A; Dabelsteen, Erik

    1999-01-01

    the function of cell-surface carbohydrates, we established organotypic cultures of skin and buccal mucosa. In these cultures, keratinocytes are grown at the air-liquid interface on a supporting matrix consisting of homologous fibroblasts embedded in a collagen type I gel. We examined the expression of blood-group...

  3. Age related blood flow around the Achilles tendon during exercise in humans

    DEFF Research Database (Denmark)

    Langberg, Henning; Olesen, J; Skovgaard, D;

    2001-01-01

    Injuries due to the overuse of tendons increase with age, and it has been suggested that this correlates with hypovascularity of the tendon. In the present study, the peritendinous blood flow was determined using xenon-133 washout at rest and during standardised intermittent exercise of the calf-muscle...

  4. Time related variations in stem cell harvesting of umbilical cord blood

    Science.gov (United States)

    Mazzoccoli, Gianluigi; Miscio, Giuseppe; Fontana, Andrea; Copetti, Massimiliano; Francavilla, Massimo; Bosi, Alberto; Perfetto, Federico; Valoriani, Alice; de Cata, Angelo; Santodirocco, Michele; Totaro, Angela; Rubino, Rosa; di Mauro, Lazzaro; Tarquini, Roberto

    2016-02-01

    Umbilical cord blood (UCB) contains hematopoietic stem cells and multipotent mesenchymal cells useful for treatment in malignant/nonmalignant hematologic-immunologic diseases and regenerative medicine. Transplantation outcome is correlated with cord blood volume (CBV), number of total nucleated cells (TNC), CD34+ progenitor cells and colony forming units in UCB donations. Several studies have addressed the role of maternal/neonatal factors associated with the hematopoietic reconstruction potential of UCB, including: gestational age, maternal parity, newborn sex and birth weight, placental weight, labor duration and mode of delivery. Few data exist regarding as to how time influences UCB collection and banking patterns. We retrospectively analyzed 17.936 cord blood donations collected from 1999 to 2011 from Tuscany and Apulia Cord Blood Banks. Results from generalized multivariable linear mixed models showed that CBV, TNC and CD34+ cell were associated with known obstetric and neonatal parameters and showed rhythmic patterns in different time domains and frequency ranges. The present findings confirm that volume, total nucleated cells and stem cells of the UCB donations are hallmarked by rhythmic patterns in different time domains and frequency ranges and suggest that temporal rhythms in addition to known obstetric and neonatal parameters influence CBV, TNC and CD34+ cell content in UBC units.

  5. Relations between diabetes, blood pressure and aortic pulse wave velocity in haemodialysis patients

    DEFF Research Database (Denmark)

    Peters, Christian Daugaard; Kjærgaard, Krista Dybtved; Dzeko, Mirela;

    Diabetes (DM) is common in haemodialysis (HD) patients and affects both blood pressure (BP) and arterial stiffness. Carotid femoral pulse wave velocity (PWV) reflects the stiffness of the aorta and is regarded as a strong risk factor for cardiovascular (CV) mortality in HD patients. However, PWV is...

  6. Related factors and prognostic significance of intradialytic blood pressure variability in patients on maintenance hemodialysis

    Institute of Scientific and Technical Information of China (English)

    谷立杰

    2013-01-01

    Objective To evaluate intradialytic blood pressure variability (BPV) in patients on maintenance hemodialysis (MHD) ,and to investigate the correlated factors of BPV in MHD process and its correlation with prognosis.Methods Patients with end stage renal disease on MHD before January 1,2009 were enrolled and analyzed retro-

  7. Rhesus Negative Woman Transfused With Rhesus Positive Blood: Subsequent Normal Pregnancy Without Anti D production.

    Science.gov (United States)

    Maya, E T; Buntugu, K A; Pobee, F; Srofenyoh, E K

    2015-03-01

    Clinicians sometimes are confronted with the challenge of transfusing haemorrhaging Rhesus (Rh) D negative patients with Rh D positive blood to save their lives. There are concerns about alloimmunization and future haemolytic disease of the newborn in women of the reproductive age. Another fear is transfusion reaction if they receive another Rh D positive blood in future. We present a 32-year-old Rh D negative woman, who had postpartum haemorrhage in her first pregnancy and was transfused with Rh D positive blood because of unavailability of Rh D negative blood. She did not receive anti D immunoglobin but subsequently had a normal term pregnancy of an Rh positive fetus without any detectable anti D antibodies throughout the pregnancy. In life threatening situations from obstetric haemorrhage, transfusion of Rh D negative women with Rh D positive blood should be considered as the last resort. PMID:26339087

  8. Effects of Montelukast on free radical production in whole blood and isolated human polymorphonuclear neutrophils (PMNs) in asthmatic children

    Science.gov (United States)

    Al Saadi, Muslim M.; Meo, Sultan Ayoub; Mustafa, Ali; Shafi, Ahmed; Tuwajri, Ali S. Al

    2011-01-01

    Montelukast is a highly selective leukotriene-receptor antagonist (LTRA). It is widely used in the treatment of bronchial asthma, primarily as an adjunct to corticosteroids. Reactive oxygen species (ROSs) play an important role in the pathogenesis of asthma and oxidative stress contributing to the initiation and worsening of inflammatory respiratory disorders, such as asthma. Antioxidant drugs may have a role in minimizing or preventing damage in asthmatic children. The aim of this study was to assess the antioxidant effect of montelukast on the production of free radicals in the whole blood and polymorphonuclear neutrophils (PMNs) in asthmatic children. A group of 48 (38 males and 10 females), apparently healthy asthmatic children were recruited with ages ranging between 6 and 14 years. In asthmatic children, base line (premedication) and post medication free radicals activity in the whole blood and polymorphonuclear neutrophils (PMNs) was determined by measuring chemiluminescence (CL) response through chemiluminescence luminometer. Free radical productions were significantly decreased in the whole blood, when stimulated with Phorbol Myristate Acetate (p < 0.04) and Opsonised Zymosan (p < 0.05). The free radicals were also significantly decreased in isolated polymorphonuclear neutrophils (PMNs) when stimulated with Opsonised Zymosan (p < 0.05) after the post medication treatment of montelukast in asthmatic children. Montelukast decreased the reactive oxygen species production, both in the whole blood as well as isolated PMNs in asthmatic children. PMID:23960762

  9. Mobilization of human hematopoietic stem/progenitor-enriched CD34+ cells into peripheral blood during stress related to ischemic stroke.

    Directory of Open Access Journals (Sweden)

    M Z Ratajczak

    2006-06-01

    Full Text Available The bone marrow-derived stem/progenitor cells were demonstrated to play an important role in a regeneration of damaged tissue. Based on these observations we asked whether the stroke-related stress triggers mobilization of stem/progenitor cells from the bone marrow into the peripheral blood, which subsequently could contribute to regeneration of damaged organs. To address this issue, the peripheral blood samples were harvested from patients with ischemic stroke during the first 24 hrs as well as after the 48 (2nd day and 144 hrs (6th day since the manifestation of symptoms. In these patients we evaluated the percentage of hematopoietic stem/progenitor-enriched CD34+ cells by employing flow cytometry and the number of hematopoietic progenitor cells for the granulocyto-monocytic (CFU-GM and erythroid (BFU-E-lineages circulating in peripheral blood. We concluded that stress related to ischemic stroke triggers the mobilization of hematopoietic stem/progenitor cells from the bone marrow into peripheral blood. These circulating stem/progenitor cells may play an important role in the process of regeneration of the ischemic tissue.

  10. EFFECTS OF AN EIGHT-WEEK ATORVASTATIN TREATMENT ON SPONTANEOUS CYTOKINE PRODUCTION BY THE BLOOD MONONUCLEAR LEUKOCYTES IN METABOLIC SYNDROME

    Directory of Open Access Journals (Sweden)

    I. D. Bespalovа

    2014-11-01

    Full Text Available The aim of this study was to assess effects of the eight-week course of atorvastatin therapy upon the levels of spontaneous cytokine production by mononuclear blood leukocytes (MNBC in metabolic syndrome. An open-label prospective study included 36 patients with stage II hypertension (blood pressure < 180/110 mm Hg. accomplished by metabolic syndrome. Along with clinical surveys performed at a specialized cardiological clinics, we assessed spontaneous cytokine production by MNBC during treatment with atorvastatin. It was shown that the 8-week treatment of these patients with atorvaststin, at individually matched daily doses (20to 40 mg was associated with reduced serum concentration of acute phase proteins (C-reactive protein and neopterin, as well as decreased spontaneous production of proinflammatory cytokines (IL-1β, IL-6 and TNFα by MNBCs. The latter finding is of great importance for pathogenesis of metabolic syndrome.

  11. Characterization of Cronobacter recovered from dried milk and related products

    Directory of Open Access Journals (Sweden)

    Fanning Séamus

    2009-02-01

    Full Text Available Abstract Background Cronobacter is a recently proposed genus consisting of six genomospecies that encompass the organisms previously identified as Enterobacter sakazakii. Cronobacter are opportunistic pathogens and are known to cause serious infections in infants, particularly neonates. High case fatality rates have been associated with infections and acute sequelae can occur in survivors with severe ramifications on neurological development. Infant formula has been identified as one route of transmission for infection in infants. However, the primary reservoirs for subsequent contamination of foods with Cronobacter remain undefined due to the ubiquitous nature of these organisms. More recently, infections in adults have been reported, especially amongst the elderly and patients who are immunocompromised. To help prevent the transmission of infection, it is important to identify the main food sources for Cronobacter. The aim of this study was to identify and characterize Cronobacter isolated from dried-milk and related products available in an Egyptian food market. Results In total sixteen Cronobacter strains were isolated from 152 dairy-based products. These were identified and characterized using pheno- and genotyping experiments. Real-time PCR confirmed the detection of Cronobacter. Following antibiotic susceptibility tests, 3 strains showed resistance to trimethoprim and/or neomycin. Phenotype profiles were generated based on key biochemical distinguishing tests. Pulsed-field gel electrophoresis (PFGE identified 8 PFGE types amongst the collection of strains. Repetitive sequence based PCR (rep-PCR analysis identified 3 rep-PCR types amongst the collection of strains. Sequencing of the recN gene was used to differentiate among the recently described species of Cronobacter. Conclusion This study identified the presence of Cronobacter in dried milk and related products sourced from the Nile-Delta region of Egypt. Although the majority of the

  12. Influence of a productive solution of uranium on some biochemical parameters of blood of an organism at experimental animals

    International Nuclear Information System (INIS)

    In the work authors inform results on studying influence of a productive solution of uranium on some biochemical parameters of blood of an organism at experimental animals. It is established that all samples of a productive solution of uranium possesses high toxicity and causes destruction of experimental animals with infringement of a picture of blood. Experiments on influence on biochemical and hematology defined parameters of blood on chickens - broilers and on which female quails from daily age raised on cellular batteries in communities on 50 heads in conditions adequate to the requirement. Fed in plenty with the dry forages prepared according to 'Recommendations on the normalized feeding an agricultural bird' (1996), recipes 5-2; 6-1. To chickens in plenty allowed water from auto drinking bowls. Conditions of a micro climate corresponded to hygienic requirements. At carrying out of experiences studied the following parameters: preservation of a livestock, the reason of a withdrawal, weight of a body - weekly up to the end of experience, consumption of a forage, an expense of a forage for 1 kg of a gain have established weight of a body the methods standard in poultry farming. After application of a productive solution took blood from under wings of a vein and in blood have established maintenance of hemoglobin, quantity of red cells, leukocytes and a leukocyte of the formula, the maintenance of calcium and phosphorus. An application of a productive solution for experimental birds for the fifth day has caused clinical symptoms of a poisoning and destruction in groups. At the survived birds in blood it has been established changes biochemical and hematology. Week age chickens of skilled group lagged behind in growth development in comparison with control group on 30 % and at the end of the skilled period skilled have lagged behind in growth and development on 62 %. Among a livestock of chickens of skilled group of a case was more on 53 % on comparisons with the

  13. Relating Productivity Events to Holocene Bivalve Shell Growth Rates

    Science.gov (United States)

    Huntley, J. W.; Krause, R. A.; Kowalewski, M.; Romanek, C. S.; Kaufman, D. S.; Simoes, M. G.

    2007-12-01

    The growth rate of a bivalve can be influenced by many environmental factors that can change during the life of the organism. In this contribution we present initial data from a millennium scale chronology to assess the relationship between ontogenetic growth in the bivalve Semele casali and paleoenvironmental conditions preserved in the shell using growth increment analysis, radiocarbon-calibrated amino acid racemization dating techniques, stable isotopes (C and O) and high spatial resolution (125-150 samples per cm of shell profile) trace element (Ba, Mn) analysis (LA-ICPMS). Time-averaged specimens of S. casali were dredged from two sites at 10 meters and 30 meters depth along the inner continental shelf at Ubatuba Bay in the Southeast Brazilian Bight, an area influenced by productivity pulses triggered by coastal runoff events and coastal upwelling. Seventy-five individual valves were dated using amino acid racemization (aspartic acid). Dates were calculated using an expanded version of a previously published relationship (Barbour Wood et al., 2006 Quaternary Research 323- 331) between aspartic acid ratios and AMS radiocarbon dates of twelve S. casali individuals from the same sampling locations. The resulting time series has complete coverage for the past three thousand years at centennial resolution. From this time series, a sub-sample of dated valves was selected for more detailed growth increment, stable isotope and high-resolution trace element (Ba/Ca and Mn/Ca) analyses. Oceanic productivity is expressed differentially in the trace element profiles of S. casali with elevated Ba/Ca and Mn/Ca ratios capturing nutrient input through coastal runoff events while elevated Ba/Ca and depressed Mn/Ca ratios represent input through coastal upwelling. Fluctuations in Ba/Ca and Mn/Ca are not correlated to fluctuations in relative growth throughout the ontogeny of an individual bivalve, nor are they expected to be as periods of increased productivity are transient

  14. PCAC Relation and Pion Production-Absorption in Nuclei

    OpenAIRE

    Nogi, T.; Sato, T; Ohtsubo, H

    2004-01-01

    Nuclear PCAC relation is studied in the framework of the effective theory of nuclear interaction, in which the interaction of real pion production-absorption is expressed by many-body operators, and does not include the one-nucleon operator as was assumed in the conventional works, while the effective axial-vector current includes the one-nucleon current in contrast to the former interaction. This problem is investigated under the simple linear $\\sigma$-model. Results are as folows: 1) The th...

  15. Relating consumer perceptions of pork quality to physical product characteristics

    DEFF Research Database (Denmark)

    Bredahl, Lone; Grunert, Klaus G.; Fertin, Claus

    1. Consumers form expectations about the quality of meat at the point of purchase based on the quality cues that are available to them in the shop. These expectations can either be confirmed or disconfirmed during consumption, depending on how cap the consumers actually are of predicting the...... related to a number of physical product characteristics commonly used to assess objective pork quality. 3. 200 German consumers are interviewed using real samples of pork chops, alongside with technical measurements of the meat quality. 4. Results show that consumers use colour, fat marbling, share of fat...

  16. A Stochastic Simulator of a Blood Product Donation Environment with Demand Spikes and Supply Shocks

    Science.gov (United States)

    An, Ming-Wen; Reich, Nicholas G.; Crawford, Stephen O.; Brookmeyer, Ron; Louis, Thomas A.; Nelson, Kenrad E.

    2011-01-01

    The availability of an adequate blood supply is a critical public health need. An influenza epidemic or another crisis affecting population mobility could create a critical donor shortage, which could profoundly impact blood availability. We developed a simulation model for the blood supply environment in the United States to assess the likely impact on blood availability of factors such as an epidemic. We developed a simulator of a multi-state model with transitions among states. Weekly numbers of blood units donated and needed were generated by negative binomial stochastic processes. The simulator allows exploration of the blood system under certain conditions of supply and demand rates, and can be used for planning purposes to prepare for sudden changes in the public's health. The simulator incorporates three donor groups (first-time, sporadic, and regular), immigration and emigration, deferral period, and adjustment factors for recruitment. We illustrate possible uses of the simulator by specifying input values for an -week flu epidemic, resulting in a moderate supply shock and demand spike (for example, from postponed elective surgeries), and different recruitment strategies. The input values are based in part on data from a regional blood center of the American Red Cross during –. Our results from these scenarios suggest that the key to alleviating deficit effects of a system shock may be appropriate timing and duration of recruitment efforts, in turn depending critically on anticipating shocks and rapidly implementing recruitment efforts. PMID:21814550

  17. A stochastic simulator of a blood product donation environment with demand spikes and supply shocks.

    Directory of Open Access Journals (Sweden)

    Ming-Wen An

    Full Text Available The availability of an adequate blood supply is a critical public health need. An influenza epidemic or another crisis affecting population mobility could create a critical donor shortage, which could profoundly impact blood availability. We developed a simulation model for the blood supply environment in the United States to assess the likely impact on blood availability of factors such as an epidemic. We developed a simulator of a multi-state model with transitions among states. Weekly numbers of blood units donated and needed were generated by negative binomial stochastic processes. The simulator allows exploration of the blood system under certain conditions of supply and demand rates, and can be used for planning purposes to prepare for sudden changes in the public's health. The simulator incorporates three donor groups (first-time, sporadic, and regular, immigration and emigration, deferral period, and adjustment factors for recruitment. We illustrate possible uses of the simulator by specifying input values for an 8-week flu epidemic, resulting in a moderate supply shock and demand spike (for example, from postponed elective surgeries, and different recruitment strategies. The input values are based in part on data from a regional blood center of the American Red Cross during 1996-2005. Our results from these scenarios suggest that the key to alleviating deficit effects of a system shock may be appropriate timing and duration of recruitment efforts, in turn depending critically on anticipating shocks and rapidly implementing recruitment efforts.

  18. Production of dendritic cells and cytokine-induced killer cells from banked umbilical cord blood samples

    Directory of Open Access Journals (Sweden)

    Phuc Van Pham

    2015-11-01

    Full Text Available Umbilical cord blood (UCB is considered to be a source of hematopoietic stem cells (HSCs. All UCB banks have recently become interested in the isolation and storage of HSCs for the treatment of hematological diseases. However, UCB was also recently confirmed as a source of immune cells for immunotherapy such as dendritic cells (DCs and cytokine-induced killer cells (CIKs. This study aimed to exploit this source of immune cells in banked UCB samples. After collection of UCB samples, mononuclear cells (MNCs containing stem cells, progenitor cells, and mature cells were isolated by Ficoll-Hypaque-based centrifugation. The MNCs were subjected to freezing and thawing according to a previously published protocol. The banked MNCs were used to produce DCs and CIKs. To produce DCs, MNCs were induced in RPMI 1640 medium supplemented with GM-CSF (50 ng/ml and IL-4 (40 ng/ml for 14 days. To produce CIKs, MNCs were induced in RPMI 1640 medium supplemented an anti-CD3 monoclonal antibody, IL-3, and GMC-SF for 21 and ndash;28 days. Both DCs and CIKs were evaluated for their phenotypes and functions according to previously published protocols. The results showed that banked UCB samples can be successfully used to produce functional DCs and CIKs. These samples are valuable sources of immune cells for immunotherapy. The present results suggest that banked UCB samples are useful not only for stem cell isolation, but also for immune cell production. [Biomed Res Ther 2015; 2(11.000: 402-408

  19. Erythrocyte-bound apolipoprotein B in relation to atherosclerosis, serum lipids and ABO blood group.

    Directory of Open Access Journals (Sweden)

    Boudewijn Klop

    Full Text Available INTRODUCTION: Erythrocytes carry apolipoprotein B on their membrane, but the determining factors of erythrocyte-bound apolipoprotein B (ery-apoB are unknown. We aimed to explore the determinants of ery-apoB to gain more insight into potential mechanisms. METHODS: Subjects with and without CVD were included (N = 398. Ery-apoB was measured on fresh whole blood samples using flow cytometry. Subjects with ery-apoB levels ≤ 0.20 a.u. were considered deficient. Carotid intima media thickness (CIMT was determined as a measure of (subclinical atherosclerosis. RESULTS: Mean ery-apoB value was 23.2% lower in subjects with increased CIMT (0.80 ± 0.09 mm, N = 140 compared to subjects with a normal CIMT (0.57 ± 0.08 mm, N = 258 (P = 0.007, adjusted P<0.001. CIMT and ery-apoB were inversely correlated (Spearman's r: -0.116, P = 0.021. A total of 55 subjects (13.6% were considered ery-apoB deficient, which was associated with a medical history of CVD (OR: 1.86, 95% CI 1.04-3.33; adjusted OR: 1.55; 95% CI 0.85-2.82. Discontinuation of statins in 54 subjects did not influence ery-apoB values despite a 58.4% increase in serum apolipoprotein B. Subjects with blood group O had significantly higher ery-apoB values (1.56 ± 0.94 a.u. when compared to subjects with blood group A (0.89 ± 1.15 a.u, blood group B (0.73 ± 0.1.12 a.u. or blood group AB (0.69 ± 0.69 a.u. (P-ANOVA = 0.002. CONCLUSION: Absence or very low values of ery-apoB are associated with clinical and subclinical atherosclerosis. While serum apolipoprotein B is not associated with ery-apoB, the ABO blood group seems to be a significant determinant.

  20. Increased micronuclei and bulky DNA adducts in cord blood after maternal exposures to traffic-related air pollution

    DEFF Research Database (Denmark)

    Pedersen, M.; Wichmann, J.; Autrup, H.;

    2009-01-01

    highest among mother-newborn pairs who lived near medium-traffic-density (> 400-2500 vehicle km/24 h; p <0.01) places. MN frequencies among newborns from women who lived at high-traffic-density homes ( > 2500 vehicle km/24 h) were significantly increased (p = 0.02). This trend remained after adjusting for......Exposure to traffic-related air pollution in urban environment is common and has been associated with adverse human health effects. In utero exposures that result in DNA damage may affect health later in life. Early effects of maternal and in utero exposures to traffic-related air pollution were...... assessed through the use of validated biomarkers in blood cells from mother-newborn pairs. A cross-sectional biomonitoring study with healthy pregnant women living in the Greater Copenhagen area, Denmark, was conducted. Bulky DNA adducts and micronuclei (MN) were measured in blood from 75 women and 69...

  1. Aircraft vibration and other factors related to high systolic blood pressure in Indonesian Air Force pilots

    Directory of Open Access Journals (Sweden)

    Minarma Siagian

    2013-05-01

    Full Text Available AbstrakLatar belakang: Penerbangan dapat berdampak pada sistem kardiovaskular manusia. Penerbang terpajan antara lain pada bising dan vibrasi pesawat. Penelitian bertujuan untuk mengetahui pengaruh beberapa faktor penerbangan pada tekanan darah sistolik.Metode: Penelitian nested case-control dilakukan pada penerbang Angkatan Udara Republik Indonesia yang melakukan pemeriksaan fisik tahunan di Lembaga Kesehatan Penerbangan dan Ruang Angkasa (LAKESPRA Saryanto tahun 2003–2008. Data yang diperoleh dari rekam medik berupa umur, jumlah jam terbang, jenis pesawat, kadar glukosa puasa dan kadar kholesterol darah, lingkaran pinggang, tinggi dan berat badan, tinggi badan, serta tekanan darah.Hasil: Dari 336 penerbang, terdapat 16 penerbanga dengan tekanan sistolik ³ 140 mmHg. Penerbang dengan rata-rata jam penerbangan 300-622 jam per tahun dibandingkan dengan 29-299 jam per tahun mempunyai risiko peningkatan tekanan darah sistolik tinggi sebesarf 5 kali [rasio odds suaian (ORa = 5,05, 95% interval kepercayaan (CI = 0,88 -23,30, P = 0,070]. Menurut jam terbang total, mereka yang memiliki 1.401-1,1125 jam dibandingkan 147-1.400 jam berisiko 3,6 kali mengalami tekanan darah sistolik tinggi (ORa = 3,58, 95% CI = 1,24-10,38. Selain itu, mereka dengan denyut nadi istirahat tinggi dibandingkan dengan denyut nadi normal istirahat memiliki 2,4 kali mengalami tekanan darah sistolik tinggi (ORa = 2,37, CI = 0,74-7,50 95, P = 0,147].Kesimpulan: Vibrasi pesawat terbang tinggi, rata-rata jam terbang per tahun tinggi, dan frekuensi nadi istirahat yang tinggi meningkatkan risiko tekanan sistolik tinggi.Kata kunci:tekanan darah sistolik, vibrasi pesawat terbang, frekuensi nadi istirahat, pilotAbstractBackground:Flight may affect the human cardiovascular system. Pilots are exposed among others to aircraft noise and vibration. This study aimed to investigate the effects of aircraft flight on systolic blood pressure.Methods:A nested case-control study was conducted on

  2. Premature culling of production animals; ethical questions related to killing animals in food production

    NARCIS (Netherlands)

    Bruijnis, M.R.N.; Meijboom, F.L.B.; Stassen, E.N.

    2016-01-01

    The aim of this chapter is to analyse the importance of longevity in relation to the welfare of production animals. I hypothesize that the concept of longevity helps to support the moral intuition that premature culling of animals is a moral wrong. The analysis shows that the interpretation of the c

  3. Role of fluid shear stress in regulating VWF structure, function and related blood disorders.

    Science.gov (United States)

    Gogia, Shobhit; Neelamegham, Sriram

    2015-01-01

    Von Willebrand factor (VWF) is the largest glycoprotein in blood. It plays a crucial role in primary hemostasis via its binding interaction with platelet and endothelial cell surface receptors, other blood proteins and extra-cellular matrix components. This protein is found as a series of repeat units that are disulfide bonded to form multimeric structures. Once in blood, the protein multimer distribution is dynamically regulated by fluid shear stress which has two opposing effects: it promotes the aggregation or self-association of multiple VWF units, and it simultaneously reduces multimer size by facilitating the force-dependent cleavage of the protein by various proteases, most notably ADAMTS13 (a disintegrin and metalloprotease with thrombospondin type repeats, motif 1 type 13). In addition to these effects, fluid shear also controls the solution and substrate-immobilized structure of VWF, the nature of contact between blood platelets and substrates, and the biomechanics of the GpIbα-VWF bond. These features together regulate different physiological and pathological processes including normal hemostasis, arterial and venous thrombosis, von Willebrand disease, thrombotic thrombocytopenic purpura and acquired von Willebrand syndrome. This article discusses current knowledge of VWF structure-function relationships with emphasis on the effects of hydrodynamic shear, including rapid methods to estimate the nature and magnitude of these forces in selected conditions. It shows that observations made by many investigators using solution and substrate-based shearing devices can be reconciled upon considering the physical size of VWF and the applied mechanical force in these different geometries. PMID:26600266

  4. Diabetes Automata For Diabetes-Related Applications: Software Engine For Blood Glucose Level Simulation

    OpenAIRE

    Agafonov, Aleksandr

    2015-01-01

    Diabetes Automata is a try of concept in the complex research-field of blood glucose simulation and prediction in experimental medical informatics, an experimental research project in software engineering combined with experimental health science. The project integrates together topics such as software system design and development, object-oriented programming, mobile application development, experimental health informatics and electronic health in general and mobile health in particular, and...

  5. The relation of blood pressure to dementia in the elderly : A community-based longitudinal study

    OpenAIRE

    Qiu, Chengxuan

    2004-01-01

    This doctoral thesis concerns a community-based, prospective study aimed at investigating the complex relationship between blood pressure (BP) and risk of dementia in a dementia-free cohort (n = 1301) aged >=75 years in Stockholm, Sweden. After baseline survey (1987-1989), the cohort was followed for over 6 years, during which 2 waves of clinical examinations (1991-1993 & 1994-1996) for dementia and Alzheimer's disease (AD) (DSM-III-R diagnostic criteria) were performed. Dat...

  6. Relation Between Red Blood Cell Omega-3 Fatty Acid Index and Bleeding During Acute Myocardial Infarction

    OpenAIRE

    Salisbury, Adam C.; Harris, William S.; Amin, Amit P.; Reid, Kimberly J.; O’Keefe, James H; Spertus, John A.

    2011-01-01

    Omega-3 fatty acids have multiple cardiovascular benefits, but may also inhibit platelet aggregation and increase bleeding risk. If this platelet inhibition is clinically meaningful, patients with the highest omega-3 indices (red blood cell eicosapentaenoic [EPA] plus docosahexaenoic acid [DHA]), which reflect long-term omega-3 fatty acid intake, should be at the greatest bleeding risk. We studied 1,523 patients from 24 US centers who had their omega-3 index assessed at the time of AMI. The r...

  7. Conditioned insulin and blood sugar responses in humans in relation to binge eating.

    Science.gov (United States)

    Overduin, J; Jansen, A

    1997-04-01

    This study proposed to demonstrate a classically conditioned blood sugar decrease in humans and to clarify its relevance for binge eating. Six conditioning trials were run in healthy females. The conditioned stimulus (CS) was a compound peppermint flavor/fragrance, whereas the unconditioned stimulus (UCS) consisted of 50 g of oral glucose. Control subjects received an aspartame drink as the UCS. Ad lib glucose intake, blood parameters, and subjective craving were monitored before and after conditioning. Results showed that the experimental group failed to show conditioned blood sugar and glucagon decreases or C-Peptide increases. Although an increased insulin response was found in the experimental group, the effect size did not exceed that of spontaneous fluctuations. No increases in craving for sweet substances were found. An impressive increase (mean: 78%) in glucose intake after conditioning was found in both conditions, as well as in a subsequently run third condition with plain water as the UCS. The increased glucose intake probably resulted from an initial neophobia to the laboratory setting that subsided as subjects had experienced more lab sessions. Importantly, because no conditioned hypoglycemia occurred in the present study, its relationship with subjectively experienced craving for sweet substance could not be determined. PMID:9108577

  8. Immune-related zinc finger gene ZFAT is an essential transcriptional regulator for hematopoietic differentiation in blood islands

    OpenAIRE

    Tsunoda, Toshiyuki; Takashima, Yasuo; Tanaka, Yoko; Fujimoto, Takahiro; Doi, Keiko; HIROSE, Yumiko; Koyanagi, Midori; Yoshida, Yasuhiro; Okamura, Tadashi; Kuroki, Masahide; Sasazuki, Takehiko; Shirasawa, Senji

    2010-01-01

    TAL1 plays pivotal roles in vascular and hematopoietic developments through the complex with LMO2 and GATA1. Hemangioblasts, which have a differentiation potential for both endothelial and hematopoietic lineages, arise in the primitive streak and migrate into the yolk sac to form blood islands, where primitive hematopoiesis occurs. ZFAT (a zinc-finger gene in autoimmune thyroid disease susceptibility region / an immune-related transcriptional regulator containing 18 C2H2-type zinc-finger doma...

  9. Relation between postoperative blood-aqueous barrier damage and LOCS III cataract gradings following routine phacoemulsification surgery

    OpenAIRE

    Ursell, P; Spalton, D; Tilling, K

    1997-01-01

    AIM—To examine the relation between cataract density, the phacoemulsification energy required for its removal, and blood-aqueous barrier (BAB) damage on the first day after surgery.
METHODS—A prospective study recruiting patients with normal eyes apart from senile cataract. Preoperatively, visual acuity, anterior chamber laser flare, and cell values were measured using the Kowa laser flare meter, and the LOCS III grading of the cataract defined. The patients all underwent standardised phacoem...

  10. The Heritability of Glaucoma-Related Traits Corneal Hysteresis, Central Corneal Thickness, Intraocular Pressure, and Choroidal Blood Flow Pulsatility

    OpenAIRE

    Freeman, Ellen E.; Roy-Gagnon, Marie-Hélène; Descovich, Denise; Massé, Hugues; Mark R. Lesk

    2013-01-01

    Purpose The purpose of this work was to investigate the heritability of potential glaucoma endophenotypes. We estimated for the first time the heritability of the pulsatility of choroidal blood flow. We also sought to confirm the heritability of corneal hysteresis, central corneal thickness, and 3 ways of measuring intraocular pressure. Methods Measurements were performed on 96 first-degree relatives recruited from Maisonneuve-Rosemont Hospital in Montreal. Corneal hysteresis was determined u...

  11. The product of resting heart rate times blood pressure is associated with high brachial-ankle pulse wave velocity.

    Directory of Open Access Journals (Sweden)

    Anxin Wang

    Full Text Available OBJECTIVE: To investigate potential associations between resting heart rate, blood pressure and the product of both, and the brachial-ankle pulse wave velocity (baPWV as a maker of arterial stiffness. METHODS: The community-based "Asymptomatic Polyvascular Abnormalities in Community (APAC Study" examined asymptomatic polyvascular abnormalities in a general Chinese population and included participants with an age of 40+ years without history of stroke and coronary heart disease. Arterial stiffness was defined as baPWV≥1400 cm/s. We measured and calculated the product of resting heart rate and systolic blood pressure (RHR-SBP and the product of resting heart rate and mean arterial pressure (RHR-MAP. RESULTS: The study included 5153 participants with a mean age of 55.1 ± 11.8 years. Mean baPWV was 1586 ± 400 cm/s. Significant (P<0.0001 linear relationships were found between higher baPWV and higher resting heart rate or higher arterial blood pressure, with the highest baPWV observed in individuals from the highest quartiles of resting heart rate and blood pressure. After adjusting for confounding parameters such as age, sex, educational level, body mass index, fasting blood concentrations of glucose, blood lipids and high-sensitive C-reactive protein, smoking status and alcohol consumption, prevalence of arterial stiffness increased significantly (P<0.0001 with increasing RHR-SBP quartile (Odds Ratio (OR: 2.72;95%Confidence interval (CI:1.46,5.08 and increasing RHR-MAP (OR:2.10;95%CI:1.18,3.72. Similar results were obtained in multivariate linear regression analyses with baPWV as continuous variable. CONCLUSIONS: Higher baPWV as a marker of arterial stiffness was associated with a higher product of RHR-SBP and RHR-MAP in multivariate analysis. In addition to other vascular risk factors, higher resting heart rate in combination with higher blood pressure are risk factors for arterial stiffness.

  12. Statistics-related and reliability-physics-related failure processes in electronics devices and products

    Science.gov (United States)

    Suhir, E.

    2014-05-01

    The well known and widely used experimental reliability "passport" of a mass manufactured electronic or a photonic product — the bathtub curve — reflects the combined contribution of the statistics-related and reliability-physics (physics-of-failure)-related processes. When time progresses, the first process results in a decreasing failure rate, while the second process associated with the material aging and degradation leads to an increased failure rate. An attempt has been made in this analysis to assess the level of the reliability physics-related aging process from the available bathtub curve (diagram). It is assumed that the products of interest underwent the burn-in testing and therefore the obtained bathtub curve does not contain the infant mortality portion. It has been also assumed that the two random processes in question are statistically independent, and that the failure rate of the physical process can be obtained by deducting the theoretically assessed statistical failure rate from the bathtub curve ordinates. In the carried out numerical example, the Raleigh distribution for the statistical failure rate was used, for the sake of a relatively simple illustration. The developed methodology can be used in reliability physics evaluations, when there is a need to better understand the roles of the statistics-related and reliability-physics-related irreversible random processes in reliability evaluations. The future work should include investigations on how powerful and flexible methods and approaches of the statistical mechanics can be effectively employed, in addition to reliability physics techniques, to model the operational reliability of electronic and photonic products.

  13. Transfusion Related Acute Lung Injury (TRALI) Caused by Red Blood Cell Transfusion Involving Residual Plasma Anti-HLA Antibodies: A report on two Cases and General Considerations

    OpenAIRE

    Olivier Garraud; Léna Absi; Danielle Rebibo; Jean-Yves Muller; Patricia Fromont; Evelyne Voitellier; Marie-Françoise Raynal; Philippe Verdier; Soizick Ducroz; Pascale Ruyer-Dumontier; Fabienne Quainon; Hélène Odent-Malaure

    2005-01-01

    TRALI is considered a serious hazard among immune complications of blood transfusion and its occurrence is admitted to be globally underestimated. Each type of blood product is likely to cause TRALI. We report here on two consecutive observations of TRALI caused by red blood cell concentrates, in which anti-HLA class I and class II antibodies resulting from post-gravitational allo-immunization were evidenced in donors. HLA class...

  14. Fibronectin adsorption on surface-modified polyetherurethanes and their differentiated effect on specific blood elements related to inflammatory and clotting processes.

    Science.gov (United States)

    Hugoni, Ludivine; Montaño-Machado, Vanessa; Yang, Meilin; Pauthe, Emmanuel; Mantovani, Diego; Santerre, J Paul

    2016-01-01

    After the introduction of a medical device into the body, adhesive proteins such as fibronectin (Fn) will adsorb to the surface of the biomaterial. Monocytes (MCs) will interact with these adsorbed proteins, and adopt either a proinflammatory and/or prowound healing phenotype, thereby influencing many blood interaction events including thrombogenesis. In this work, Fn adsorption as well as subsequent MC response and thrombus formation were investigated on two surfaces-modified polyetherurethanes (PEUs) using different surface modifiers: an anionic/dihydroxyl oligomeric (ADO) additive, known to enable cell adhesion, and a fluorinated polypropylene oxide oligomer (PPO), known to reduce platelet adhesion. Results indicated that at 24 h of MC culture, PEU-ADO and PEU-PPO promoted an anti-inflammatory character relative to the base PEU. Longer clotting times, based on a free hemoglobin assay, were also found on the two surface-modified PEUs relative to the native one, suggesting their potential for the reduction of thrombus formation. In presence of a Fn monolayer, the surface-modified PEUs conserved a lower thrombogenic character than the base PEU, and was however significantly decreased when compared to prior protein adsorption. Furthermore, Fn coatings increased the MC production levels of tumor necrosis factor-α and interleukin-10 at 24 h, while not affecting the anti-inflammatory effect of the modifications relative to the base PEU. This finding was most prominent on PEU-PPO, suggesting that the interaction of the adsorbed Fn with blood cells was different for the two additives. Hence, the results highlighted differentiating effects of Fn adsorption on specific blood activating processes related to inflammatory and thrombotic responses. PMID:27246517

  15. [Correlation between Staphylococcus carriage, specific antibody-production and AB0-blood grouping in plasma donors].

    Science.gov (United States)

    Nemyrovs'ka, L M; Patoka, V V

    2002-01-01

    Interaction peculiarities of three components of the immune human homeostasis-antigens of blood groups AB0, staphylococcus antigens and antistaphylococcus antibodies have been investigated. Donors (85) of antistaphylococcus plasma immunized by staphylococcus anatoxin have been investigated. It is found that the nasal staphylococcus carriage in donors depends on the level of specific and natural antibodies and on the coincidence between the staphylococcus antigen structure and the protein substance of the specific blood group factors. PMID:12190026

  16. Omega-3 fatty acids improve psychomotor performance via mechanism not related to nitric acid production

    Directory of Open Access Journals (Sweden)

    Marwan S. M. Al-Nimer

    2012-01-01

    Full Text Available Omega-3 fatty acids (ω-3FAs are essential polyunsaturated fats that protect the brain from cognitive impairment. It increases the activity of endothelial nitric oxide synthetase (eNOS and thereby increases the nitric acid (NO production. This study aimed to explore the effect of ω-3FAs on psychomotor performance and to relate this effect to the reactive nitrogen species. This study was conducted in Department of Pharmacology, College of Medicine, Al-Mustansiriya University in Baghdad, Iraq. Twenty healthy subjects, allocated randomly from medical college students, were participated in the single blind clinical trial. Participants were divided into two groups, each of ten subjects to receive either placebo or (ω-3FAs (750 mg single oral dose daily for 5 days. They were asked to perform psychomotor performance before and after 5 days of treatment, and venous blood was obtained for determination of serum nitric oxide (NO and peroxynitrite (ONOO. ω-3FAs treated group was significantly different from placebo-treated group in reducing choice and motor reaction times as well as the critical flicker frequency threshold. The serum levels of NO and ONOO in ω-3FAs-treated group did not significantly differ from placebo-treated group. Short term supplementation of ω-3FAs improves the psychomotor performance in young healthy subjects via a mechanism not related to the production of nitric oxide production. Inflorescence is a panicle few flowered and fruit is a capsule. The data of the results obtained were presented and discussed.

  17. The effects of aprotinin on blood product transfusion associated with thoracic aortic surgery requiring deep hypothermic circulatory arrest.

    LENUS (Irish Health Repository)

    Seigne, P W

    2012-02-03

    OBJECTIVE: To compare the effects of aprotinin on blood product use and postoperative complications in patients undergoing thoracic aortic surgery requiring deep hypothermic circulatory arrest. DESIGN: A retrospective study. SETTING: A university hospital. PARTICIPANTS: Nineteen patients who underwent elective or urgent thoracic aortic surgery. INTERVENTIONS: None. MEASUREMENTS AND MAIN RESULTS: The total number of units of packed red blood cells, fresh frozen plasma, and platelets was significantly less in the group that received aprotinin (p = 0.01, 0.04, and 0.01). The intraoperative transfusion of packed red blood cells and platelets, collection and retransfusion of cell saver, and postoperative transfusion of fresh frozen plasma were also significantly less in the aprotinin group (p = 0.01, 0.02, 0.01, and 0.05). No patient in either group sustained renal dysfunction or a myocardial infarction. Two patients who had not received aprotinin suffered from chronic postoperative seizures, and one patient who had received aprotinin sustained a perioperative stroke. CONCLUSIONS: Low-dose aprotinin administration significantly decreases blood product transfusion requirements in the setting of thoracic aortic surgery requiring deep hypothermic circulatory arrest, and it does not appear to be associated with renal or myocardial dysfunction.

  18. Blood Products and the Commodification Debate: The Blurry Concept of Altruism and the 'Implicit Price' of Readily Available Body Parts.

    Science.gov (United States)

    Dufner, Annette

    2015-12-01

    There is a widespread consensus that a commodification of body parts is to be prevented. Numerous policy papers by international organizations extend this view to the blood supply and recommend a system of uncompensated volunteers in this area--often, however, without making the arguments for this view explicit. This situation seems to indicate that a relevant source of justified worry or unease about the blood supply system has to do with the issue of commodification. As a result, the current health minister of Ontario is proposing a ban on compensation even for blood plasma--despite the fact that Canada can only generate 30 % of the plasma needed for fractionation into important plasma protein products and has to purchase the rest abroad. In the following, I am going to suggest a number of alternative perspectives on the debate in order to facilitate a less dogmatic and more differentiated debate about the matter. Especially in light of the often over-simplified notions of altruism and commodification, I conclude that the debate has not conclusively established that it would be morally objectionable to provide blood plasma donors with monetary compensation or with other forms of explicit social recognition as an incentive. This is especially true of donations for fractionation into medicinal products by profit-oriented pharmaceutical companies. PMID:25471706

  19. Signal pathways underlying homocysteine-induced production of MCP-1 and IL-8 in cultured human whole blood

    Institute of Scientific and Technical Information of China (English)

    Xiao-kun ZENG; You-fei GUAN; Daniel G REMICK; Xian WANG

    2005-01-01

    Aim: To elucidate the mechanisms underlying homocysteine (Hcy)-induced chemokine production. Methods: Human whole blood was pretreated with inhibitors of calmodulin (CaM), protein kinase C (PKC), protein tyrosine kinase(PTK), mitogen-activated protein kinase (MAPK), and NF-κB and activators of PPARγ for 60 min followed by incubation with Hcy 100 μmol/L for 32 h. The levels of mitogen chemokine protein (MCP)-1 and interleukin-8 (IL-8) were determined by enzyme-linked immunosorbant assay (ELISA). Results: Inhibitors of PKC (calphostin C, 50-500 nmol/L and RO-31-8220, 10-100 nmol/L), CaM(W7, 28-280 μmol/L), ERK1/2 MAPK (PD 98059, 2-20 μmol/L), p38 MAPK(SB 203580, 0.6-6 μmol/L), JNK MAPK (curcumin, 2-10 μmol/L), and NF-κB(PDTC, 10-100 nmol/L) markedly reduced Hcy 100 μmol/L-induced production of MCP-1 and IL-8 in human cultured whole blood, but the inhibitors of PTK(genistein, 2.6-26 μmol/L and tyrphostin, 0.5-5 μmol/L) had no obvious effect on MCP-1 and IL-8 production. PPARγ activators (ciglitazone 30 μmol/L and troglitazone 10 μmol/L) depressed the Hcy-induced MCP-1 production but not IL-8 production in the cultured whole blood. Conclusion: Hcy-induced MCP-1 and IL-8 production is mediated by activated signaling pathways such as PKC,CaM, MAPK, and NF-κB. Our results not only provide clues for the signal transduction pathways mediating Hcy-induced chemokine production, but also offer a plausible explanation for a pathogenic role of hyperhomocysteinemia in these diseases.

  20. Relationship of efficiency indices with performance, heart rate, oxygen consumption, blood parameters, and estimated heat production in Nellore steers.

    Science.gov (United States)

    Chaves, A S; Nascimento, M L; Tullio, R R; Rosa, A N; Alencar, M M; Lanna, D P

    2015-10-01

    The objective of this study was to examine the relationship of efficiency indices with performance, heart rate, oxygen consumption, blood parameters, and estimated heat production (EHP) in Nellore steers. Eighteen steers were individually lot-fed diets of 2.7 Mcal ME/kg DM for 84 d. Estimated heat production was determined using oxygen pulse (OP) methodology, in which heart rate (HR) was monitored for 4 consecutive days. Oxygen pulse was obtained by simultaneously measuring HR and oxygen consumption during a 10- to 15-min period. Efficiency traits studied were feed efficiency (G:F) and residual feed intake (RFI) obtained by regression of DMI in relation to ADG and midtest metabolic BW (RFI). Alternatively, RFI was also obtained based on equations reported by the NRC's to estimate individual requirement and DMI (RFI calculated by the NRC [1996] equation [RFI]). The slope of the regression equation and its significance was used to evaluate the effect of efficiency indices (RFI, RFI, or G:F) on the traits studied. A mixed model was used considering RFI, RFI, or G:F and pen type as fixed effects and initial age as a covariate. For HR and EHP variables, day was included as a random effect. There was no relationship between efficiency indices and back fat depth measured by ultrasound or daily HR and EHP ( > 0.05). Because G:F is obtained in relation to BW, the slope of G:F was positive and significant ( RFI and RFI ( RFI. Oxygen consumption per beat was not related to G:F; however, it was lower for RFI- and RFI-efficient steers, and consequently, oxygen volume (mL·min·kg) and OP (μL O·beat·kg) were also lower ( RFI and RFI ( > 0.05); however, G:F-efficient steers showed lower hematocrit and hemoglobin concentrations ( < 0.05). Differences in EHP between efficient and inefficient animals were not directly detected. Nevertheless, differences in oxygen consumption and OP were detected, indicating that the OP methodology may be useful to predict growth efficiency. PMID

  1. Comprehensive kinetics of triiodothyronine production, distribution, and metabolism in blood and tissue pools of the rat using optimized blood-sampling protocols.

    Science.gov (United States)

    DiStefano, J J; Jang, M; Malone, T K; Broutman, M

    1982-01-01

    We have determined estimates for 24 physiological parameters of production, interpool transport, distribution, and metabolism of T3 in the major T3 pools of the unanesthetized male Sprague-Dawley rat, from blood-borne data and a comprehensive model and analysis of this system. Most of these indices have previously been unavailable. Whereas only 3% (2 ng/100 g BW) of the total body T3 pool (74 ng/100 g BW) is in plasma, the composite of slowly equilibrating (slow) tissue pools (e.g. muscle, skin, and brain) appears to contain most of the T3, 76% (57 ng/100 g BW) of the total. The composite of rapidly equilibrating (fast) tissue pools (e.g. liver and kidney) contains the remaining 19% (16 ng/100 g BW). The total body T3 production rate is 0.12 ng/100 g BW . min, and we estimate that about half of this emanates directly from T4 in the slow pools, whereas the remainder is derived from both thyroidal secretion and T4 to T3 conversion in the fast pools. Our results also indicate that T3 molecules spend an average of only 0.5 min in transit each time through plasma, whereas the single pass mean transit times in fast and slow tissue pools (the times available for hormone action) are 10 times and 200 times greater. In contrast, the mean residence time for T3 in the entire system is greater than 12 h despite the extremely rapid early disappearance of injected T3 from plasma. To obtain the required accuracy, we used a novel optimization approach for choosing blood-sampling schedules (1, 4, 44, 202, and 600 min), a remarkably small number of sample times, and each was adjustable by about +/- 20% without effect on optimized parameter accuracies. PMID:7053984

  2. Dose related anxiolytic effects of diazepam: relation with serum electrolytes, plasma osmolality and systolic blood pressure (sbp) in rats

    International Nuclear Information System (INIS)

    Diazepam is an anxiolytic and anticonvulsant drug that also induces hypnosis. Changes in serum electrolyte balance, plasma osmolality and systolic blood pressure (SBP) are often associated with stress-induced anxiety. Administration of diazepam has been show to decrease stress-induced enhancement of hypothalamic pituitary adrenal cortical (HPA) axis. The present is designed to monitor the anxiolytic effects of different doses of diazepam (1 mg/kg, 2.5 mg/kg and 5 mg/kg) and its association with changes of serum electrolyte balance, plasma osmolality and SBP in rats. Administration of diazepam at doses of 1 mg/kg, 2.5 mg/kg and 5 mg/kg elicited anxiolytic effects monitored in light-dark transition test and increased serum concentration of electrolytes and plasma osmolality. Serum levels of magnesium as well as SBP decreased. The results are discussed in context of anxiolytic effects of diazepam to be mediated via a modulation of stress-induced increase in the activity of HPA-axis arid electrolytes balance. (author)

  3. Blood pressure-lowering effects of beetroot juice and novel beetroot-enriched bread products in normotensive male subjects

    OpenAIRE

    Hobbs, Ditte; Kaffa, Nedi; George, Trevor; Methven, Lisa; Lovegrove, Julie

    2012-01-01

    A number of vegetables have a high nitrate content which after ingestion can be reduced to nitrite by oral bacteria, and further to vasoprotectiveNO endogenously. In the present study, two separate randomly controlled, single-blind, cross-over, postprandial studies wereperformed in normotensive volunteers. Ambulatory blood pressure (BP) was measured over a 24 h period following consumption of eitherfour doses of beetroot juice (BJ), 0, 100, 250 and 500 g (n 18), or three bread products, contr...

  4. Biochemical indices of blood serum of cows with acute radiation sickness due to nuclear fission products. [/sup 235/U

    Energy Technology Data Exchange (ETDEWEB)

    Yakovleva, V.P.; Burov, N.I

    1977-01-01

    Fission products of /sup 235/U were given to cows daily for 4 days. The animals developed the intestinal form of acute radiation sickness and did not survive longer than 20 days. Blood samples were taken at intervals and determinations were made on activity of transaminases and alkaline phosphatase, serum carbohydrates, cholesterol, lecithin, total lipids, K, Na, and Fe. Results showed an increase in enzyme activity, increase in carbohydrate levels, decrease in lipids and electrolytes, and weight loss. (HLW)

  5. Inhibition of endotoxin-induced interleukin-6 production by synthetic lipid A partial structures in human peripheral blood mononuclear cells.

    OpenAIRE

    Wang, M. H.; Flad, H D; Feist, W; Brade, H; Kusumoto, S; Rietschel, E T; Ulmer, A J

    1991-01-01

    The effect of two synthetic lipid A partial structures, compound 406 (or LA-14-PP, identical in structure to the lipid A precursor, known as Ia or IVa) and compound 401 (lipid X), on the in vitro modulation of endotoxin (lipopolysaccharide)-induced interleukin-6 production by human blood mononuclear cells was investigated. Lipopolysaccharide of Salmonella abortus equi and synthetic Escherichia coli-type lipid A (compound 506, or LA-15-PP) had potent interleukin-6-inducing capacities. The maxi...

  6. Productive performance and blood parameters of bulls fed diets containing babassu mesocarp bran and whole or ground corn

    OpenAIRE

    Aline Evangelista Machado Santana; José Neuman Miranda Neiva; João Restle; Fabrícia Rocha Chaves Miotto; Luciano Fernandes Sousa; Vera Lúcia Araújo; Ranniere Rodrigues Pereira Parente; Rhaiza Alves de Oliveira

    2015-01-01

    The objective of the present study was to evaluate the effects of corn milling and the inclusion of babassu mesocarp bran (BMB) on productive performance, digestibility of dietary nutrients, and blood parameters of dairy crossbred (Holstein-Gyr) bulls finished in confinement. Twenty-four bulls were fed four different experimental diets, containing two levels of inclusion of BMB (0 and 41.24%) and corn supplied in two different forms (ground and whole), for 98 days (77 days of data collection ...

  7. Age-related macular degeneration is associated with increased proportion of CD56(+) T cells in peripheral blood

    DEFF Research Database (Denmark)

    Faber, Carsten; Singh, Amardeep; Krüger Falk, Mads; Juel, Helene Bæk; Sørensen, Torben Lykke; Nissen, Mogens Holst

    2013-01-01

    PURPOSE: To examine the association between age-related changes in the T-cell compartment and prevalence of age-related macular degeneration (AMD). DESIGN: Case-control study. PARTICIPANTS: A total of 117 AMD cases and 106 controls were included prospectively. METHODS: Fresh-drawn peripheral blood...... levels of circulating aged CD56(+) CD28(-) T cells in patients with AMD. Although this supports the notion of AMD as a systemic disease, it also suggests that the adaptive immune system is implicated in its pathogenesis....

  8. High Homocysteine and Blood Pressure Related to Poor Outcome of Acute Ischemia Stroke in Chinese Population

    Science.gov (United States)

    Liu, Changjiang; Zhao, Liang; Zhou, Mo; Sun, Wenjie; Xu, Tan; Tong, Weijun

    2014-01-01

    Objectives To assess the association between plasma homocysteine (Hcy), blood pressure (BP) and poor outcome at hospital discharge among acute ischemic stroke patients, and if high Hcy increases the risk of poor outcome based on high BP status in a northern Chinese population. Methods Between June 1, 2009 and May 31, 2013, a total of 3695 acute ischemic stroke patients were recruited from three hospitals in northern Chinese cities. Demographic characteristics, lifestyle risk factors, medical history, and other clinical characteristics were recorded for all subjects. Poor outcome was defined as a discharge modified Rankin Scale (mRS) score ≥3 or death. The association between homocysteine concentration, admission blood pressure, and risk of poor outcome following acute ischemic stroke was analyzed by using multivariate non-conditional logistic regression models. Results Compared with those in the lowest quartile of Hcy concentration in a multivariate-adjusted model, those in the highest quartile of Hcy concentration had increased risk of poor outcome after acute ischemic stroke, (OR = 1.33, P<0.05). The dose-response relationship between Hcy concentration and risk of poor outcome was statistically significant (p-value for trend  = 0.027). High BP was significantly associated with poor outcome following acute ischemic stroke (adjusted OR = 1.44, 95%CI, 1.19–1.74). Compared with non-high BP with nhHcy, in a multivariate-adjusted model, the ORs (95% CI) of non-high BP with hHcy, high BP with nhHcy, and high BP with hHcy to poor outcome were 1.14 (0.85–1.53), 1.37 (1.03–1.84) and 1.70 (1.29–2.34), respectively. Conclusion The present study suggested that high plasma Hcy and blood pressure were independent risk factors for prognosis of acute ischemic stroke, and hHcy may further increase the risk of poor outcome among patients with high blood pressure. Additionally, the results indicate that high Hcy with high BP may cause increased susceptibility

  9. Pretreatment blood concentrations of chloroquine in patients with malaria infection: relation to response to treatment

    DEFF Research Database (Denmark)

    Quashie, Neils Ben; Akanmori, Bartholomew D; Goka, Bamenla Q;

    2005-01-01

    malaria infection in Ghana, we hypothesized that the 'added effect' of the pretreatment ingested drug to the full dose given at the hospital may be responsible for the low proportion of RIII type of resistance observed. To ascertain this, pretreatment blood levels of chloroquine were correlated with...... of malaria in children in Ghana, in the presence of chloroquine resistance.......Resistance of Plasmodium falciparum to chloroquine has been reported in many areas in Ghana. Most of these reports, which are from hospital-based studies, indicate RI and RII rather than RIII type of resistance. Since high pretreatment levels of chloroquine have also been measured in patients with...

  10. Gender Differences Relating to Metabolic Syndrome and Proinflammation in Finnish Subjects with Elevated Blood Pressure

    OpenAIRE

    Esko Kumpusalo; Mauno Vanhala; Hannu Kautiainen; Tiina Ahonen; Juha Saltevo; Markku Laakso

    2009-01-01

    Fasting insulin, adiponectin, high-sensitivity C-reactive protein (hs-CRP), and interleukin-1 receptor antagonist (IL-1Ra) were determined in 278 men and 273 women with blood pressure ≥130 and/or ≥85 mmHg and/or with antihypertensive medication. Metabolic syndrome (MetS) with the National Cholesterol Education Program (NCEP) criteria was observed in 35% of men and 34% of women. Men with MetS had lower hs-CRP and IL-1Ra than women. The absolute gender difference in adiponectin was smaller and ...

  11. ALBANIAN CUSTOMARY LAW IN RELATION WITH BLOOD FEUD, IMPACT OF CANON AND LAW IN ITS PREVENTION.

    OpenAIRE

    NASER SOPJANI

    2014-01-01

    Albanians still occur assassinations and which in the opinion presented as blood feud murders committed by and on behalf of the canon, as murders committed due to the lack of force of law. Are such these murders, and as is acceptable to the people that they were carried out according to of customary law, canon, it is another thing!The Albanian customary law through the main canons summarized and codified, has clearly defined categories and ways of murder, who can be killed, when they can be k...

  12. The heritability of glaucoma-related traits corneal hysteresis, central corneal thickness, intraocular pressure, and choroidal blood flow pulsatility.

    Directory of Open Access Journals (Sweden)

    Ellen E Freeman

    Full Text Available PURPOSE: The purpose of this work was to investigate the heritability of potential glaucoma endophenotypes. We estimated for the first time the heritability of the pulsatility of choroidal blood flow. We also sought to confirm the heritability of corneal hysteresis, central corneal thickness, and 3 ways of measuring intraocular pressure. METHODS: Measurements were performed on 96 first-degree relatives recruited from Maisonneuve-Rosemont Hospital in Montreal. Corneal hysteresis was determined using the Reichert Ocular Response Analyser. Central corneal thickness was measured with an ultrasound pachymeter. Three measures of intraocular pressure were obtained: Goldmann-correlated and corneal compensated intraocular pressure using the Ocular Response Analyser, and Pascal intraocular pressure using the Pascal Dynamic Contour Tonometer. The pulsatility of choroidal blood velocity and flow were measured in the sub-foveolar choroid using single-point laser Doppler flowmetry (Oculix. We estimated heritability using maximum-likelihood variance components methods implemented in the SOLAR software. RESULTS: No significant heritability was detected for the pulsatility of choroidal blood flow or velocity. The Goldman-correlated, corneal compensated, and Pascal measures of intraocular pressure measures were all significantly heritable at 0.94, 0.79, and 0.53 after age and sex adjustment (p = 0.0003, p = 0.0023, p = 0.0239. Central corneal thickness was significantly heritable at 0.68 (p = 0.0078. Corneal hysteresis was highly heritable but the estimate was at the upper boundary of 1.00 preventing us from giving a precise estimate. CONCLUSION: Corneal hysteresis, central corneal thickness, and intraocular pressure are all heritable and may be suitable as glaucoma endophenotypes. The pulsatility of choroidal blood flow and blood velocity were not significantly heritable in this sample.

  13. Blood transfusion economics in Greece.

    Science.gov (United States)

    Kyriopoulos, J E; Michail-Merianou, V; Gitona, M

    1995-01-01

    This paper describes the organizational structure and the economic impact of blood donation and transfusion in Greece and discusses some alternative aspects of its financing and its costing policy. The cost of blood transfusion is rising in Greece and amounts to nearly 15 billion drachmas per year due to the constant increase in demand and consequently, the price of each unit of blood. The production and distribution of blood on national scale involves meeting the demand for 500.000 units. Blood is mostly given by the friends and relatives of patients (55%) and by voluntary blood donation (30%). Approximately 50% of the blood produced is used in surgery, 20% for cases of beta-thalassaemia, 10% for emergencies and 20% for internal medicine cases. The blood transfusion system is totally funded by the state budget and the value to users is free of charge. The way in which blood is collected and processed differs from one geographical area to another and the unit cost depends on the size of the department concerned, ranging from 60-150 $. The need to control costs and restrain expenditure, in conjunction with guarantees of sufficiency and quality, makes it essential that measures should be taken to introduce economies of scale and encourage competition among blood providers, for increased production, components preparation and rational usage of blood. The introduction of a costing policy becomes necessary in this effort to achieve cost-containment techniques. PMID:8581182

  14. Alteration of Peripheral Blood T-Reg Cells and Cytokines Production in Angiography Personnel Exposed to Scattered X-Rays

    Directory of Open Access Journals (Sweden)

    Ebrahim Torkabadi

    2007-12-01

    Full Text Available Angiocardiography is an X-ray examination of the blood vessels or chambers of the heart. Cardiologists and staff members applying this procedure are exposed to high levels of scattered radiation. In our previous study the incidence of unstable chromosomal aberrations and cytokinesis-blocked micronuclei were found to be significantly higher in exposed individuals than the age and sex matched controls. In the present study we assessed cytokine production by peripheral blood mononuclear cells of the above cases and the percentage of Treg cells. According to film dosimeter analysis, personnels received 0.25-15 mSv during the previous year (average of 3 mSv/y. Isolated PBMCs from the test and control groups were stimulated with Phorbol Myristate Acetate/ Ionomycin (PMA/I. Cytokine production was measured in the supernatants of cultured lymphocytes. The percentage of Treg cells was studied by flow cytometry. The production of IL-10 and IL-5 was significantly down-regulated in the test group compared to the control group. In contrast, IL-12 was up-regulated. Yet, no statistically significant difference was found for IFN- γ between two groups. In addition, we found higher percentage of CD4+CD25+bright Treg cells in the study group compared to the controls. Taken together, it was shown that low doses of scattered X-rays could skew cytokine profile of peripheral blood mononuclear cells in favour of inflammatory response causing the increase of Treg cells.

  15. Preconcentration and determination of lead and cadmium levels in blood samples of adolescent workers consuming smokeless tobacco products in Pakistan.

    Science.gov (United States)

    Arain, Sadaf Sadia; Kazi, Tasneem Gul; Afridi, Hassan Imran; Brahman, Kapil Dev; Naeemullah; Khan, Sumaira; Panhwar, Abdul Haleem; Kamboh, Muhammad Afzal; Memon, Jamil R

    2015-05-01

    The present study was aimed to evaluate the cadmium (Cd) and lead (Pb) levels in the blood samples of adolescent boys, chewing different smokeless tobacco (SLT) products in Pakistan. For comparative purpose, boys of the same age group (12-15 years), not consumed any SLT products were selected as referents. To determine trace levels of Cd and Pb in blood samples, a preconcentration method, vortex-assisted liquid-liquid microextraction (VLLME) has been developed, prior to analysis by flame atomic absorption spectrometry. The hydrophobic chelates of Cd and Pb with ammonium pyrrolidinedithiocarbamate were extracted into the fine droplets of ionic liquid (IL) 1-butyl-3-methylimidazolium hexafluorophosphate, while nonionic surfactant, Triton X-114 was used as a dispersing medium. The main factors affecting the recoveries of Cd and Pb, such as concentration of APDC, centrifugation time, volume of IL and TX-114, were investigated in detail. It was also observed that adolescent boys who consumed different SLT products have 2- to 3-fold higher levels of Cd and Pb in their blood samples as compared to referent boys (p < 0.001). PMID:25930204

  16. Nucleic Acid, Antibody, and Virus Culture Methods to Detect Xenotropic MLV-Related Virus in Human Blood Samples

    Directory of Open Access Journals (Sweden)

    M. F. Kearney

    2011-01-01

    Full Text Available The MLV-related retrovirus, XMRV, was recently identified and reported to be associated with both prostate cancer and chronic fatigue syndrome. At the National Cancer Institute-Frederick, MD (NCI-Frederick, we developed highly sensitive methods to detect XMRV nucleic acids, antibodies, and replication competent virus. Analysis of XMRV-spiked samples and/or specimens from two pigtail macaques experimentally inoculated with 22Rv1 cell-derived XMRV confirmed the ability of the assays used to detect XMRV RNA and DNA, and culture isolatable virus when present, along with XMRV reactive antibody responses. Using these assays, we did not detect evidence of XMRV in blood samples ( or prostate specimens ( from two independent cohorts of patients with prostate cancer. Previous studies detected XMRV in prostate tissues. In the present study, we primarily investigated the levels of XMRV in blood plasma samples collected from patients with prostate cancer. These results demonstrate that while XMRV-related assays developed at the NCI-Frederick can readily measure XMRV nucleic acids, antibodies, and replication competent virus, no evidence of XMRV was found in the blood of patients with prostate cancer.

  17. EFSA Panel on Dietetic Products, Nutrition and Allergies (NDA); Scientific Opinion on the substantiation of a health claim related to a combination of plant sterols and Cholesternorm®mix and reduction of blood LDL-cholesterol concentrations pursuant to Article 14 of Regulation (EC) No 1924/2006

    DEFF Research Database (Denmark)

    Tetens, Inge

    provides at the levels of the proposed conditions of use around 0.52 g plant sterols, 0.95 g linoleic acid, 0.13 g alpha-linolenic acid and 0.13 g pectins per day. The combination of plant sterols and Cholesternorm®mix, which is the subject of the claim, is sufficiently characterised in relation to the...

  18. EFSA NDA Panel (EFSA Panel on Dietetic Products, Nut rition and Allergies ) , 2013. Scientific Opinion on the substantiation of a health claim related to the c onsum ption of 2 g/day of plant stanols (as plant stanol ester s ) as part of a diet low in saturated fat and a two - fold greater, reduction in blood LDL - chol esterol concentrations compared to the consumption of a diet low in saturated fat alone pursuant to Article 14 of Regulation (EC) No 1924/2006

    DEFF Research Database (Denmark)

    Tetens, Inge

    diet low in saturated fat alone. The food that is the subject of the health claim, plant stanol esters, is sufficiently characterised. The applicant provided five human intervention studies for the scientific substantiation of the claim. The Panel notes that the design of the studies submitted did not...... to deliver an opinion on the scientific substantiation of a health claim related to the consumption of 2 g/day of plant stanols (as plant stanol esters) as part of a diet low in saturated fat and a two-fold greater reduction in blood LDL-cholesterol concentrations compared to the consumption of a......Following an application from McNeil Nutritionals and Raisio Nutrition Ltd, submitted for authorisation of a health claim pursuant to Article 14 of Regulation (EC) No 1924/2006 via the Competent Authority of the United Kingdom, the Panel on Dietetic Products, Nutrition and Allergies (NDA) was asked...

  19. EFSA NDA Panel (EFSA Panel on Dietetic Products, Nutrition and Allergies ), 2013. Scientific Opinion on the substantiation of a health claim related to the combination of artichoke leaf dry extract standardised in caffeoylquinic acids, monacolin K in red yeast ric e, sugar - cane derived, policosanols, OPC from French maritime pine bark, garlic dry extract standardised in allicin, d - α - tocopheryl hydrogen succinate , riboflavin and inositol hexanicotinate in Limicol ® and reduction of blood LDL - cholesterol concentrations pursuant to Article 14 of Regulation (EC) No 1924/2006

    DEFF Research Database (Denmark)

    Tetens, Inge

    claim related to the combination of artichoke leaf dry extract standardised in caffeoylquinic acids, monacolin K in red yeast rice, sugar-cane derived policosanols, OPC from French maritime pine bark, garlic dry extract standardised in allicin, d-α-tocopheryl hydrogen succinate, riboflavin and inositol...... ingredients in Limicol® on blood LDL-cholesterol concentrations. The Panel concludes that a cause and effect relationship has been established between the consumption of the combination of artichoke leaf dry extract standardised in caffeoylquinic acids, monacolin K in red yeast rice, sugar-cane derived......Following an application from Laboratoire Lescuyer, submitted pursuant to Article 14 of Regulation (EC) No 1924/2006 via the Competent Authority of France, the Panel on Dietetic Products, Nutrition and Allergies (NDA) was asked to deliver an opinion on the scientific substantiation of a health...

  20. Microalbuminuria in patients with non-insulin-dependent diabetes mellitus relates to nocturnal systolic blood pressure.

    LENUS (Irish Health Repository)

    Mitchell, T H

    2012-02-03

    PURPOSE: Microalbuminuria predicts early mortality in non-insulin-dependent-diabetes mellitus patients (NIDDM). Our objective in the present study was to compare and assess the relationship between 24-hour, day and nocturnal ambulatory blood pressure (BP) and urinary albumin excretion rate (UAE) in microalbuminuric and normoalbuminuric NIDDM and in normal control subjects. PATIENTS AND METHODS: In the present cross-sectional study, 24 hour ambulatory BP (daytime BP and nocturnal BP) and HbA1c were compared in microalbuminuric (n = 10) and nonmicroalbuminuric NIDDM patients (n = 10) and in nondiabetic controls (n = 9). None of the patients were taking antihypertensive agents. RESULTS: In the microlbuminuric group, whereas 24 hour and daytime systolic BP differed significantly from control values (P < 0.025 and P < 0.05 respectively), there was no difference between diabetic groups. However, nocturnal systolic BP in the microalbuminuric group was significantly higher than in the normoalbuminuric diabetic patients (139 vs. 125) (P < 0.05) and a significant difference was also found between the NIDDM patients and the control group (139, 125 vs. 114) (P < 0.025). In multiple regression analysis, only nocturnal systolic BP showed a significant relationship with UAE (P < 0.05). CONCLUSIONS: We suggest that the higher nocturnal systolic blood pressure seen in our microalbuminuric NIDDM patients may contribute to the increased morbidity in this group.

  1. EVALUATION OF IL-6 PRODUCTION BY HUMAN BLOOD CELLS INCUBATED WITH METAL COMPLEXES OF Γ-GLOBULIN

    OpenAIRE

    S. B. Cheknev; I. E. Efremova; A. S. Mezdrokhina; A. A. Babajanz

    2014-01-01

    Abstract. This study has shown that that a common cytokine pool induced in cultured human peripheral blood cells (PBC) supplied by either γ-globulin fraction proteins, copper or zinc cations, or appropriate metal complexes, contains detectable amounts of IL-6 (0.39+0.14 to 2.04+0.16 ng/ml). γ-globulin complexes with zinc or copper ions are able to induce production of IL-6 in amounts differing from those induced by control proteins, or copper and zinc ions used alone. IL-6 production by PBC i...

  2. Interleukin-4 and interferon-¿ production by Leishmania stimulated peripheral blood mononuclear cells from nonexposed individuals

    DEFF Research Database (Denmark)

    Kurtzhals, J A; Kemp, M; Poulsen, L K;

    1995-01-01

    Interferon-gamma (IFN-gamma) and interleukin-4 (IL-4) production by Leishmania reactive peripheral blood mononuclear cells (PBMC) from non-exposed individuals was investigated. IFN-gamma was measured in culture supernatants after antigen stimulation. For the measurement of IL-4, antigen stimulated...... Leishmania reactive CD4+ T cells could be demonstrated. The cells from different individuals showed different patterns of IFN-gamma and/or IL-4 production upon antigenic stimulation. In experimental leishmaniasis the early balance between IFN-gamma and IL-4 is important for the clinical outcome. Our findings...

  3. Sulphydryl groups and their relation to the antioxidant enzymes of chelonian red blood cells.

    Science.gov (United States)

    Torsoni, M A; Viana, R I; Ogo, S H

    1998-09-01

    Thiol groups of hemoglobin and blood glutathione are higher in Geochelone carbonaria than in Geochelone denticulata. Exposure of stripped hemolysate of both tortoises to terc-butyl hydroperoxide, resulted in a higher ferroheme oxidation of G. denticulata hemoglobin. In this example glutathione reductase and glutathione peroxidase, were not active due to the absence of GSH and NADPH, suggesting that the thiol groups of G. carbonaria hemoglobin act as antioxidant, similar to GSH. In the total hemolysate, however, where the antioxidant enzymes are active, both species showed similar levels of hemoglobin oxidation, suggesting that the protective effect of thiol groups of hemoglobin are less effective for heme protection. The activity of glutathione reductase and glutathione peroxidase was higher in erythrocytes of G. denticulata and the activity of catalase and superoxide dismutase was higher in erythrocytes of G. carbonaria. PMID:9784849

  4. Whole blood hypoxia-related gene expression reveals novel pathways to obstructive sleep apnea in humans.

    Science.gov (United States)

    Perry, Juliana C; Guindalini, Camila; Bittencourt, Lia; Garbuio, Silverio; Mazzotti, Diego R; Tufik, Sergio

    2013-12-01

    In this study, our goal was to identify the key genes that are associated with obstructive sleep apnea (OSA). Thirty-five volunteers underwent full in-lab polysomnography and, according to the sleep apnea hypopnea index (AHI), were classified into control, mild-to-moderate OSA and severe OSA groups. Severe OSA patients were assigned to participate in a continuous positive airway pressure (CPAP) protocol for 6 months. Blood was collected and the expression of 84 genes analyzed using the RT(2) Profiler™ PCR array. Mild-to-moderate OSA patients demonstrated down-regulation of 2 genes associated with induction of apoptosis, while a total of 13 genes were identified in severe OSA patients. After controlling for body mass index, PRPF40A and PLOD3 gene expressions were strongly and independently associated with AHI scores. This research protocol highlights a number of molecular targets that might help the development of novel therapeutic strategies. PMID:23994550

  5. IFNγ production in peripheral blood of early Lyme disease patients to hLFAαL (aa326-345

    Directory of Open Access Journals (Sweden)

    Wormser Gary P

    2002-10-01

    Full Text Available Abstract Background It has been proposed that outer surface protein A (OspA of Borrelia burgdorferi sensu stricto contains a T helper 1 (Th1 cell epitope that could play a role in an autoimmune response to hLFA1. Methods We used two peptides, hLFAαL (aa326-345 and Borrelia burgdorferi OspAB31 (aa164-183, as stimulating antigens to measure Th1 proinflammatory IFNγ cytokine production in peripheral blood of Lyme disease patients presenting with EM without history of arthritis, as well as in peripheral blood of healthy individuals. Results IFNγ responses to hLFA1 peptide were observed in 11 of 19 Lyme disease patients and in 3 of 15 healthy controls. In contrast, only 2 of 19 of the Lyme disease patients and none of the controls responded to the homologous OspAB31 peptide. Conclusions IFNγ was produced in response to stimulation with peptide hLFAαL (aa326-345 in peripheral blood of 58% of patients with early Lyme disease without signs of arthritis, as well as in peripheral blood of 20% of healthy individuals, but not in response to stimulation with the homologous OspAB31 (aa164-183 peptide (p

  6. Successful second transplantation from haploidentical donor for graft failure following unrelated cord blood cell transplantation or mismatched related transplantation: 2cases report

    Institute of Scientific and Technical Information of China (English)

    XU Lan-ping; HUANG Xiao-jun

    2006-01-01

    @@ Cord blood transplantation (CBT) from unrelated donors has increasingly been performed worldwide during the last decade. The immaturity of lymphocytes in cord blood permits HLA-mismatching between donors and recipients and reduces the severity of graft-versus-host disease (GVHD).However, the relatively small dose of the cord blood nucleated cells is associated with a high frequency of engraftment failure.1-5 But re-transplantation with stem cells from the original donor is impossible.

  7. Effect of advanced glycosylation end products on activity of protein kinase C in human peripheral blood mononuclear cells

    Institute of Scientific and Technical Information of China (English)

    2000-01-01

    Objectives TO investigate the effect of advanced glycosylation end products (AGEs) on the activity of protein kinese C (PKC) in human peripheral bloodmononuclear Cells (PBMC) and to observe whether aminoguanidine (AG) can influence the effect of AGEs. Methods After PBMC were isoiated from human peripheral blood and incubated with different concentrations of AGEs-BSA for various periods, total PKC activity in PBMC was determined by measuring the incorporation of 32P from [γ-32P] ATP=into a special substrate using Prornega PKC assay kit. Results AGEs-BSA increased the total PKC activity in PBMC from 83.43±6.57 pmol/min/mg protein to 116.8±13.82 pmol/min/mg protein with a peak at 15 min.AGEs-BSA also increased the total PKC activity in a concentration-dependent manner from 83.1±6.4 pmol/min/mg protein(control) to 119.1±13.3 pmol/min/mg protein (control vs AGEs-BSA 400 mg/L, P<0.01). Furthermore, AGEs-BSA induced an elevation of PKC activity in a glycosylating time-related manner,from 80.9±8.2 (control) to 118.3±11.5 pmol/min/mg protein (glycasytation for 12 wk, P<0.01). The total PKC activity stimulated by AGEs-BSA pretreated with AG (100, 200 mg/L) was markedly lower than that of AGEs-BSA group not pretreated with AG ( P<0.05, P<0.01). Conclusions AGEs-BSA increased the total PKC activity in PBMC in a concentration and incubation time dependent manner. The ability of AGEs-B.SA to stimulate PKC activity was markedly decreased by pretreatment of AGEs-BSA with AG.

  8. Thymus cells in myasthenia gravis selectively enhance production of anti-acetylcholine-receptor antibody by autologous blood lymphocytes

    International Nuclear Information System (INIS)

    We investigated the role of the thymus in 16 patients with myasthenia gravis without thymoma by studying the production of anti-acetylcholine-receptor antibody by thymic and blood lymphocytes cultured alone or together. In 10 responders (with the highest receptor-antibody titers in their plasma), cultured thymic cells spontaneously produced measurable receptor antibody. Receptor-antibody production by autologous blood lymphocytes was enhanced by the addition of responder's thymic cells, irradiated to abrogate antibody production and suppression (P<0.01). This enhancement was greater and more consistent than that by pokeweed mitogen; it depended on viable thymic cells, appeared to be selective for receptor antibody, and correlated with the ratio of thymic helper (OKT4-positive or OKT4+) to suppressor (OKT8+) T cells (P<0.01). These results suggest that myasthenic thymus contains cell-bound acetylcholine-receptor-like material or specific T cells (or both) that can aid receptor-antibody production. This may be relevant to the benefits of thymectomy in myasthenia and to the breakdown in self-tolerance in this and other autoimmune diseases

  9. Thymus cells in myasthenia gravis selectively enhance production of anti-acetylcholine-receptor antibody by autologous blood lymphocytes

    Energy Technology Data Exchange (ETDEWEB)

    Newsom-Davis, J.; Willcox, N.; Calder, L.

    1981-11-26

    We investigated the role of the thymus in 16 patients with myasthenia gravis without thymoma by studying the production of anti-acetylcholine-receptor antibody by thymic and blood lymphocytes cultured alone or together. In 10 responders (with the highest receptor-antibody titers in their plasma), cultured thymic cells spontaneously produced measurable receptor antibody. Receptor-antibody production by autologous blood lymphocytes was enhanced by the addition of responder's thymic cells, irradiated to abrogate antibody production and suppression (P<0.01). This enhancement was greater and more consistent than that by pokeweed mitogen; it depended on viable thymic cells, appeared to be selective for receptor antibody, and correlated with the ratio of thymic helper (OKT4-positive or OKT4+) to suppressor (OKT8+) T cells (P<0.01). These results suggest that myasthenic thymus contains cell-bound acetylcholine-receptor-like material or specific T cells (or both) that can aid receptor-antibody production. This may be relevant to the benefits of thymectomy in myasthenia and to the breakdown in self-tolerance in this and other autoimmune diseases.

  10. Relative indexes of cutaneous blood perfusion measured by real-time laser Doppler imaging (LDI) in healthy volunteers.

    Science.gov (United States)

    Seyed Jafari, S Morteza; Schawkat, Megir; Van De Ville, Dimitri; Shafighi, Maziar

    2014-07-01

    We used real-time LDI to study regional variations in microcirculatory perfusion in healthy candidates to establish a new methodology for global perfusion body mapping that is based on intra-individual perfusion index ratios. Our study included 74 (37 female) healthy volunteers aged between 22 and 30 years (mean 24.49). Imaging was performed using a recent microcirculation-imaging camera (EasyLDI) for different body regions of each volunteer. The perfusion values were reported in Arbitrary Perfusion Units (APU). The relative perfusion indexes for each candidate's body region were then obtained by normalization with the perfusion value of the forehead. Basic parameters such as weight, height, and blood pressure were also measured and analyzed. The highest mean perfusion value was reported in the forehead area (259.21APU). Mean perfusion in the measured parts of the body correlated positively with mean forehead value, while there was no significant correlation between forehead blood perfusion values and room temperature, BMI, systolic blood pressure and diastolic blood pressure (p=0.420, 0.623, 0.488, 0.099, respectively). Analysis of the data showed that perfusion indexes were not significantly different between male and female volunteers except for the ventral upper arm area (p=.001). LDI is a non-invasive, fast technique that opens several avenues for clinical applications. The mean perfusion indexes are useful in clinical practice for monitoring patients before and after surgical interventions. Perfusion values can be predicted for different body parts for patients only by taking the forehead perfusion value and using the perfusion index ratios to obtain expected normative perfusion values. PMID:24788075

  11. Guidelines for the Deployment of Product-Related Environmental Legislation into Requirements for the Product Development Process

    DEFF Research Database (Denmark)

    Ferraz, Mariana; Antelmi Pigosso, Daniela Cristina; Teixeira, Cláudia Echevenguá; Rozenfeld, Henrique

    2013-01-01

    Environmental legislation is increasingly changing its focus from end-of-pipe approaches to a life cycle perspective. Therefore, manufacturing companies are increasingly identifying the need of deploying and incorporating product-related environmental requirements into the product development pro...... process. This paper presents twelve guidelines, clustered into three groups, to support companies in the identification, analysis and deployment of product requirements from product-related environmental legislation.......Environmental legislation is increasingly changing its focus from end-of-pipe approaches to a life cycle perspective. Therefore, manufacturing companies are increasingly identifying the need of deploying and incorporating product-related environmental requirements into the product development...

  12. Effects of MDMA and related analogs on plasma 5-HT: Relevance to 5-HT transporters in blood and brain

    OpenAIRE

    Yubero-Lahoz, Samanta; Ayestas, Mario A; Blough, Bruce E; Partilla, John S; Rothman, Richard B.; de la Torre, Rafael; Baumann, Michael H.

    2011-01-01

    (±)-3,4-Methylenedioxymethamphetamine (MDMA) is an illicit drug that evokes transporter-mediated release of serotonin (5-HT) in the brain. 5-HT transporter (SERT) proteins are also expressed in non-neural tissues (e.g., blood), and evidence suggests that MDMA targets platelet SERT to increase plasma 5-HT. Here we tested two hypotheses related to the effects of MDMA on circulating 5-HT. First, to determine if MDMA metabolites might contribute to actions of the drug in vivo, we used in vitro mi...

  13. Relationship between ketosis and dairy cows' blood metabolites in intensive production farms of the periurban area of Dakar.

    Science.gov (United States)

    Yameogo, Nongasida; Ouedraogo, Georges Anicet; Kanyandekwe, Christine; Sawadogo, Germain Jerome

    2008-10-01

    This study which involved 140 Holstein and Montbeliard was carried out in the periurban area of Dakar with the aim to establish the relationship between ketosis, milk production and biochemical blood metabolites. The results showed that ketosis is a real problem in periurban farms around the city of Dakar with high proportions of 33.57% for subclinical ketosis and 6.43% for clinical ketosis. In their second month of milking, cows with subclinical ketosis had a decrease of 12.4 and 15.,6% in milk yield respectively for Montbeliard and Holstein, whereas cows with clinical ketosis had a decrease of 18.6 and 26%. Ketogenic cows (subclinical and clinical) have significantly lower average levels of blood glucose (pfarm to another, significant difference was recorded with concentration of total proteins and globulin, calcium and magnesium. PMID:18716904

  14. Impact of Brazilian red propolis extract on blood metabolites, milk production, and lamb performance of Santa Inês ewes.

    Science.gov (United States)

    Morsy, Amr S; Soltan, Yosra A; Sallam, Sobhy M A; Alencar, Severino M; Abdalla, Adibe L

    2016-06-01

    Twenty Santa Inês ewes used to evaluate effects of oral administration of Brazilian red propolis extract on blood metabolites, milk production, and lamb performance were randomly grouped (n = 10 ewes/group) to control without propolis administration and propolis treated (3 g red propolis extract/ewe/day) 21 days before expected lambing date. Blood samples were collected weekly, and daily milk yield was recorded twice weekly until 7 weeks postpartum. Propolis administration increased (P protein, and globulin and glucose concentrations, decreased (P milk, fat, protein, and lactose. Propolis supplementation increased (P milk conversion ratio but had no effect on lamb birth and weaning weights. The prepartum administration of propolis extract supported positively the transition of ewes from pregnancy to lactation with health benefits achieved for both of ewes and lambs performances. PMID:27098314

  15. Reference ranges and age-related changes of peripheral blood lymphocyte subsets in Chinese healthy adults

    Institute of Scientific and Technical Information of China (English)

    JIAO Yang; QIU ZhiFeng; XIE Jing; LI DongJing; LI TaiSheng

    2009-01-01

    This study was performed to build region-specific reference ranges of peripheral blood lymphocyte subsets for Chinese healthy adults from the young to the elderly and analyze the trends of changes in lymphocyte subsets for evaluating the impact of age on the values. 151 healthy adults aged 19-86 were recruited based on the SENIEUR protocol. Three sets of reference ranges were finally built applicable for the healthy young (19-44 years), middle-aged (45-64 years) and elder adults (>65). Comparisons in parameters among the three cohorts showed that e statistically significant increase in CD16CD56+ NK cell was observed between the middle-aged and elder cohorts, whereas for the majority of the parameters, a significant decline was observed between the young and the middle-aged cohorts.Further results showed that inverse correlations were observed between the age and CD19+ B, CD3+T,CD3+CD4+1, CD4+CD45RA+CD62L+ naTve T cell and CD4+CD28+/CD4+, while the positive one was identified between the age end the NK cell. These significant changes of the most of immune parameters provided evidence for immunosenescence. Notably, T cell activation markers of CD8+CD38+ and CD8+HLA-DR+ showed reverse trends of association with age, which provides a clue for further researches on the mechanisms underlying the paradoxical clinical presentation of the elder patients.

  16. Smoking-related microRNAs and mRNAs in human peripheral blood mononuclear cells.

    Science.gov (United States)

    Su, Ming-Wei; Yu, Sung-Liang; Lin, Wen-Chang; Tsai, Ching-Hui; Chen, Po-Hua; Lee, Yungling Leo

    2016-08-15

    Teenager smoking is of great importance in public health. Functional roles of microRNAs have been documented in smoke-induced gene expression changes, but comprehensive mechanisms of microRNA-mRNA regulation and benefits remained poorly understood. We conducted the Teenager Smoking Reduction Trial (TSRT) to investigate the causal association between active smoking reduction and whole-genome microRNA and mRNA expression changes in human peripheral blood mononuclear cells (PBMC). A total of 12 teenagers with a substantial reduction in smoke quantity and a decrease in urine cotinine/creatinine ratio were enrolled in genomic analyses. In Gene Set Enrichment Analysis (GSEA) and Ingenuity Pathway Analysis (IPA), differentially expressed genes altered by smoke reduction were mainly associated with glucocorticoid receptor signaling pathway. The integrative analysis of microRNA and mRNA found eleven differentially expressed microRNAs negatively correlated with predicted target genes. CD83 molecule regulated by miR-4498 in human PBMC, was critical for the canonical pathway of communication between innate and adaptive immune cells. Our data demonstrated that microRNAs could regulate immune responses in human PBMC after habitual smokers quit smoking and support the potential translational value of microRNAs in regulating disease-relevant gene expression caused by tobacco smoke. PMID:27321975

  17. Relating external compressing pressure to mean arterial pressure in non-invasive blood pressure measurements.

    Science.gov (United States)

    Chin, K Y; Panerai, R B

    2015-01-01

    Arterial volume clamping uses external compression of an artery to provide continuous non-invasive measurement of arterial blood pressure. It has been assumed that mean arterial pressure (MAP) corresponds to the point where unloading leads to the maximum oscillation of the arterial wall as reflected by photoplethysmogram (PPG), an assumption that has been challenged. Five subjects were recruited for the study (three males, mean age (SD) = 32 (15) years). The PPG waveform was analysed to identify the relationship between the external compressing pressure, PPG pulse amplitude and MAP. Two separate tests were carried out at compression step intervals of 10 mmHg and 2 mmHg, respectively. No significant differences were found between the two tests. The bias between the compressing pressure and the MAP was -4.7 ± 5.63 mmHg (p < 0.001) showing a normal distribution. Further research is needed to identify optimal algorithms for estimation of MAP using PPG associated with arterial compression. PMID:25429784

  18. Effects of cooling on certain blood constituents in relation to reproductive behaviour in buffaloes

    Science.gov (United States)

    Mehta, S. N.; Gangwar, P. C.; Srivastava, R. K.; Dhingra, D. P.

    1981-09-01

    Forty-five healthy lactating buffaloes were taken for this study in three groups: group 1 served as control. At 11:00 and 15:00 h daily the animals in group 2 were given showers and of group 3 were sent for wallowing for half an hour each time. Blood samples were taken at 14-day intervals. The overall mean values of acid phosphatase, alkaline phosphatase, total plasma ascorbic acid and plasma proteins were 2.61, 2.87, 2.89 (KA U/100ml plasma); 4.81, 4.71, 4.51 (KA U/100 ml plasma), 1.04, 1.29, 1.41 (mg/100ml plasma) and 7.05, 6.17, 6.05 (g/100 ml plasma) in control, shower and wallow groups respectively. The different parameters of reproductive efficiency-percentage of animals coming in oestrus, days-open, duration of oestrus, percent conception rate and services per conception were 40, 60, 19.09, 20, 7 in the controlgroup; 53.33, 42, 19.46, 28.50, 4.25 in the shower group and 73.33, 38.75, 23.00, 50 and 2.00 in the wallow group respectively.

  19. [Antibody production in the blood of donors immunized with staphylococcal anatoxin].

    Science.gov (United States)

    Dyzyk, H M; Shumeha, I S; Tarasenko, A O; Patoka, V V

    1998-01-01

    Blood serum content was studied of specific antistaphylococcal antibodies (staphylolysins) in 576 donors immunized with staphylococcal anatoxin with the purpose of obtaining an antistaphylococcal plasma and antistaphylococcal immunoglobulin to be used in clinical settings. 292 donors had been immunized and examined prior to 1986, 284--after 1986 (before 1994). Comparison of the immune responses in the above periods of time permitted finding out that 13.03% of immunized donors responded to the antigenic stimulus by such paradoxical reaction as disappearance of specific antibodies; the number of persons-active respondents has gotten reduced from 17.12% to 5.98% as has the number of individuals having the baseline level of staphylolysins (1-2 ME/ml). The changes were at their greatest in donors with group A (II) blood. PMID:9621632

  20. Higher blood flow and circulating NO products offset high-altitude hypoxia among Tibetans

    OpenAIRE

    Erzurum, S. C.; Ghosh, S; Janocha, A. J.; W Xu; Bauer, S.; Bryan, N. S.; Tejero, J.; Hemann, C.; Hille, R; Stuehr, D J; Feelisch, M; Beall, C. M.

    2007-01-01

    The low barometric pressure at high altitude causes lower arterial oxygen content among Tibetan highlanders, who maintain normal levels of oxygen use as indicated by basal and maximal oxygen consumption levels that are consistent with sea level predictions. This study tested the hypothesis that Tibetans resident at 4,200 m offset physiological hypoxia and achieve normal oxygen delivery by means of higher blood flow enabled by higher levels of bioactive forms of NO, the main endothelial factor...

  1. The influences of SE infection on layers’ production performance, egg quality and blood biochemical indicators

    OpenAIRE

    Fan, Shijie; Zheng, Jiangxia; Duan, Zhongyi; Yang, Ning; Xu, Guiyun

    2014-01-01

    Background Salmonella enterica serovar Enteritidis (SE), as a major cause of foodborn illness, infects humans mainly through the egg. However, the symptom of laying hens usually is not typical and hard to diagnosis. In the present study, it is studied that the influences of SE infection on layers’ performance, egg quality and blood biochemical indicators. It will help us to improve the strategy to control SE infection in commercial layers. One hundred layers at 20 wk of age were divided into ...

  2. Milk production, intake, digestion, blood parameters, and ingestive behavior of cows supplemented with by-products from the biodiesel industry.

    Science.gov (United States)

    Gonzaga Neto, Severino; Oliveira, Ronaldo Lopes; de Lima, Francisco Helton Sá; de Medeiros, Ariosvaldo Nunes; Bezerra, Leilson Rocha; Viégas, Júlio; do Nascimento, Nilton Guedes; de Freitas Neto, Marcondes Dias

    2015-01-01

    This study aimed to evaluate the intake, digestion, blood parameters, and feeding behavior of crossbred dairy cows (Holstein × Gir) managed on Panicum maximum Jacq. cv. Tanzania-1 and provided supplementation with groundnut cake, sunflower cake, or palm kernel cake (to replace soybean meal). Sixteen cows were randomly assigned in a Latin square design with four treatments and four experimental periods. The consumption of nutrients from the pasture did not vary between experimental treatments. Cows receiving the palm kernel cake supplement had a reduced crude protein, non-fibrous carbohydrate, and total digestible nutrient intake and an increase in the average consumption of ether extract. There was also a reduction in the digestibility of dry matter. The inclusion of supplements in the diet did not influence the average time spent eating, ruminating, or resting. The mean values of respiratory and heart rates showed thermal comfort during the trial period. There was a reduction in blood urea nitrogen with palm kernel cake supplementation, and the values of total protein, albumin, and glucose were also significantly different with this supplement. It is recommended that cakes of groundnut cake and sunflower cake seed be used for a total replacement of soybean meal supplements for lactating cows, but the use of palm kernel cake is not recommended. PMID:25319453

  3. Significant association between ABO blood group and pancreatic cancer

    Institute of Scientific and Technical Information of China (English)

    Julia; B; Greer; Mark; H; Yazer; Jay; S; Raval; M; Michael; Barmada; Randall; E; Brand; David; C; Whitcomb

    2010-01-01

    AIM:To evaluate whether the ABO blood group is related to pancreatic cancer risk in the general population of the United States.METHODS:Using the University of Pittsburgh's clinicalpancreatic cancer registry,the blood donor database from our local blood bank (Central Blood Bank),and the blood product recipient database from the regional transfusion service (Centralized Transfusion Service) in Pittsburgh,Pennsylvania,we identified 274 pancreatic cancer patients with previously determined serological ABO bloo...

  4. Reference ranges and age-related changes of peripheral blood lymphocyte subsets in Chinese healthy adults

    Institute of Scientific and Technical Information of China (English)

    2009-01-01

    This study was performed to build region-specific reference ranges of peripheral blood lymphocyte subsets for Chinese healthy adults from the young to the elderly and analyze the trends of changes in lymphocyte subsets for evaluating the impact of age on the values.151 healthy adults aged 19-86 were recruited based on the SENIEUR protocol.Three sets of reference ranges were finally built applicable for the healthy young(19-44 years),middle-aged(45-64 years) and elder adults(≥65).Comparisons in parameters among the three cohorts showed that a statistically significant increase in CD16CD56+ NK cell was observed between the middle-aged and elder cohorts,whereas for the majority of the parameters,a significant decline was observed between the young and the middle-aged cohorts.Further results showed that inverse correlations were observed between the age and CD19+ B,CD3+ T,CD3+CD4+ T,CD4+CD45RA+CD62L+ nave T cell and CD4+CD28+/CD4+,while the positive one was identified between the age and the NK cell.These significant changes of the most of immune parameters provided evidence for immunosenescence.Notably,T cell activation markers of CD8+CD38+ and CD8+HLA-DR+ showed reverse trends of association with age,which provides a clue for further researches on the mechanisms underlying the paradoxical clinical presentation of the elder patients.

  5. Influence of Salt Intake on Association of Blood Uric Acid with Hypertension and Related Cardiovascular Risk.

    Directory of Open Access Journals (Sweden)

    Lei Hou

    Full Text Available A relationship of blood uric acid (UA with hypertension and cardiovascular risk is under debate thus salt intake is hypothesized to contribute to such associations.In this cross-sectional study, stratified cluster random sampling elicited a sample of 1805 Kazakhs with 92.4% compliance. Hypertension and moderate-or-high total cardiovascular risk (mTCR were defined according to guidelines. Sodium intake was assessed by urinary sodium excretion. Prevalence ratios (PRs were used to express associations of UA with hypertension and mTCR.In the highest tertile of sodium intake in women, the adjusted PRs (95% confidence intervals of low to high quartiles compared with the lowest quartile of UA, were 1.22(0.78-1.91, 1.18(0.75-1.85, and 1.65(1.09-2.51 for hypertension and 1.19(0.74-1.90, 1.39(0.91-2.11, and 1.65(1.10-2.47 for mTCR (P for trend <0.05. However, these findings were not shown for other sodium intake levels. There were similar results in men. PRs markedly increased with a concomitant increase in UA and sodium intake and there was a significant interaction (P = 0.010 for mTCR with PRs of 1.69(1.10-2.60 for men and 3.70(2.09-6.52 for women in those with the highest compared with the lowest quartile of UA and tertile of sodium intake. Similar findings were shown for hypertension.This study implied that a high salt intake may enhance the associations of UA with hypertension and cardiovascular risk.

  6. Radionuclide Angiography for Detection of Relative Differences in Renal Blood Flow

    International Nuclear Information System (INIS)

    A technique is described to detect differences in blood flow between kidneys using 99mTc pertechnetate and the Pho-Gamma III Scintillation Camera. The scintigraphic studies were performed on normal subjects and patients presenting mainly hypertension due to renal disease. After the catheterization of the femoral artery a catheter was inserted in the aorta a little higher than the point of origin of the renal arteries. Renal scintiphotography, including the sequential filling studies, was performed by the manual operation of the camera, and utilization of the special attachments, such as the 1600 word memory and magnetic tape recorder. 15 mCi of 99mTc in a volume not greater than 1.5 ml were injected rapidly into the renal arteries through the catheter. The scintiphotographs were taken at the 2nd, 5th, 10th and 20th second after injection. Simultaneously the counts were collected at intervals of 0.02 min, digitized by the 1600 word memory and transferred to the magnetic tape recorder from which the data could be played back at any time for further analysis. From the sequence of the digital images taken, two areas of interest corresponding to the renal arteries were selected. The sum of the counts of the areas was taken for the. whole series of the radioarteriograms. Finally, an evaluation of the change of the concentration of radioactivity in the selected areas was made by plotting the sum of counts versus time. Thus two curves were taken corresponding to both renal arteries. These curves express, quantitatively, the progressive filling of the arteries as a function of time. By taking the ratio of the sum of the two arteries a curve is plotted which demonstrates objectively differences in the concentration of radioactivity between the two arteries. (author)

  7. Relation of Blood Pressure to Retinal Vessel Diameter in Type 1 Diabetes Mellitus

    Science.gov (United States)

    Klein, Ronald; Myers, Chelsea E.; Klein, Barbara E. K.; Zinman, Bernard; Gardiner, Robert; Suissa, Samy; Sinaiko, Alan R.; Donnelly, Sandra M.; Goodyer, Paul; Strand, Trudy; Mauer, Michael

    2009-01-01

    Objective To examine the relationship of blood pressure (BP) and use of angiotensin receptor blocker (ARB) or angiotensin converting enzyme inhibitor (ACEI) to retinal vessel diameter in normotensive, normoalbuminuric persons with type 1 diabetes mellitus (T1DM). Design Randomized controlled clinical trial. Participants Persons with T1DM and gradable fundus photographs both at baseline (n=147) and 5-year follow-up (n=124). Methods Clinic and 24-hour ambulatory BPs (ABP) were measured. Retinal arteriolar and venular diameters were measured using a computer-assisted technique. Individual arteriolar and venular measurements were combined into summary indices that reflect the average retinal arteriolar (central retinal arteriolar equivalent [CRAE]) and venular (central retinal venular equivalent [CRVE]) diameter of an eye, respectively. Main Outcome Measures CRAE and CRVE. Results While controlling for age, study site, glycosylated hemoglobin and ambulatory pulse rate, daytime ambulatory systolic (-0.29 μm effect per 1mmHg, P=.02) and daytime ambulatory diastolic (-0.44 μm effect per 1mmHg, P=.04), nighttime ambulatory systolic (-0.27 μm effect per 1mmHg, P=.03), and 24-hour ambulatory systolic BP (-0.31 μm effect per 1mmHg, P=.03) were cross-sectionally associated with a smaller CRAE. While controlling for age, study site, glycosylated hemoglobin, ambulatory pulse rate and baseline CRAE, no BP measure was associated with a change in CRAE or CRVE over 5 years of follow-up. Treatment with losartan or enalapril was not associated with a statistically significant change in CRAE or CRVE. Conclusions ACEI or ARB therapy does not affect retinal arteriolar or venular diameter in normotensive persons with T1DM. PMID:20142543

  8. Visit-to-Visit Variability in Blood Pressure Is Related to Late-Life Cognitive Decline.

    Science.gov (United States)

    Qin, Bo; Viera, Anthony J; Muntner, Paul; Plassman, Brenda L; Edwards, Lloyd J; Adair, Linda S; Popkin, Barry M; Mendez, Michelle A

    2016-07-01

    The association between visit-to-visit variability of blood pressure (BP) and cognitive decline over time remains incompletely understood in a general population of older adults. We assessed the hypothesis that higher visit-to-visit variability in BP, but not mean BP, would be associated with faster decline in cognitive function among community-dwelling older adults. This prospective cohort study comprised 976 adults who had 3 or 4 visits with BP measurements as part of the China Health and Nutrition Survey from 1991, up to their first cognitive tests, and completed cognitive screening tests at ≥2 visits in 1997, 2000, or 2004. Visit-to-visit BP variability was expressed as the SD, coefficient of variation, or as the variation independent of mean BP across visits conducted at a mean interval of 3.2 years. Mean (SD) age at the first cognitive test was 64 (6) years. Using multivariable-adjusted linear mixed-effects models, we found higher visit-to-visit variability in systolic BP, but not mean systolic BP, was associated with a faster decline of cognitive function (adjusted mean difference [95% confidence interval] for high versus low tertile of SD variability: standardized composite scores -0.038 standard units (SU)/y [-0.066 to -0.009] and verbal memory -0.041 SU/y [-0.075 to -0.008]). Higher visit-to-visit variability in diastolic BP was associated with a faster decline of cognitive function, independent of mean diastolic BP, among adults aged 55 to 64 years but not those ≥65 years. Our results suggest that higher long-term BP visit-to-visit variability is associated with a faster rate of cognitive decline among older adults. PMID:27217401

  9. Blood donor management in china.

    Science.gov (United States)

    Shi, Ling; Wang, Jingxing; Liu, Zhong; Stevens, Lori; Sadler, Andrew; Ness, Paul; Shan, Hua

    2014-07-01

    Despite a steady increase in total blood collections and voluntary non-remunerated blood donors, China continues to have many challenges with its blood donation system. The country's donation rate remains low at 9%o, with over 60% of donors being first-time donors. Generally there is a lack of adequate public awareness about blood donation. The conservative donor selection criteria, the relatively long donation interval, and the small donation volume have further limited blood supply. To ensure a sufficient and safe blood supply that meets the increasing clinical need for blood products, there is an urgent need to strengthen the country's blood donor management. This comprehensive effort should include educating and motivating more individuals especially from the rural areas to be involved in blood donation, developing rational and evidence-based selection criteria for donor eligibility, designing a donor follow-up mechanism to encourage more future donations, assessing the current donor testing strategy, improving donor service and care, building regional and national shared donor deferral database, and enhancing the transparency of the blood donation system to gain more trust from the general public. The purpose of the review is to provide an overview of the key process of and challenges with the blood donor management system in China. PMID:25254023

  10. Intravesical BCG therapy in bladder carcinoma. Effect on cytotoxicity, IL-2 production and phenotype of peripheral blood mononuclear cells

    DEFF Research Database (Denmark)

    Hermann, G G; Petersen, K R; Zeuthen, J; Steven, K

    1991-01-01

    The purpose of the study was to examine the effects of intravesical BCG treatment on the cytotoxicity, interleukin-2 (IL-2) production and distribution of the subsets of peripheral blood mononuclear cells (PBMC) in patients with carcinoma in situ of the bladder. Treatments were made in 6 patients...... during a conventional BCG treatment schedule. Four patients showed a complete response, one a partial response and one had a progressive disease after BCG treatment. Intravesical BCG did not induce significant changes in the cytotoxicity of PBMC. The distribution of NK-cells and T-cells also remained...

  11. High Blood Pressure (Hypertension)

    Science.gov (United States)

    ... Radiation-Emitting Products Vaccines, Blood & Biologics Animal & Veterinary Cosmetics Tobacco Products For Consumers Home For Consumers Consumer Information by Audience For Women High Blood Pressure (Hypertension) Share Tweet Linkedin Pin ...

  12. Genes related to xylose fermentation and methods of using same for enhanced biofuel production

    Science.gov (United States)

    Wohlbach, Dana J.; Gasch, Audrey P.

    2014-08-05

    The present invention provides isolated gene sequences involved in xylose fermentation and related recombinant yeast which are useful in methods of enhanced biofuel production, particularly ethanol production. Methods of bioengineering recombinant yeast useful for biofuel production are also provided.

  13. Genes related to xylose fermentation and methods of using same for enhanced biofuel production

    Energy Technology Data Exchange (ETDEWEB)

    Wohlbach, Dana J.; Gasch, Audrey P.

    2015-09-29

    The present invention provides isolated gene sequences involved in xylose fermentation and related recombinant yeast which are useful in methods of enhanced biofuel production, particularly ethanol production. Methods of bioengineering recombinant yeast useful for biofuel production are also provided.

  14. Ex vivo measurement of calpain activation in human peripheral blood lymphocytes by detection of immunoreactive products of calpastatin degradation.

    Directory of Open Access Journals (Sweden)

    Jacek M Witkowski

    2008-01-01

    Full Text Available Limited proteolysis of multiple intracellular proteins by endogenous Ca-dependent cysteine proteases--calpains--is an important regulatory mechanism for cell proliferation, apoptosis etc. Its importance for cellular functions is stressed by existence of endogenous calpain inhibitors--calpastatins. The calpain-calpastatin system within living cells is in a fragile balance, which depends on both partners. The interdependence of calpain--a protease--and calpastatin--an endogenous inhibitor and at the same time a substrate for this enzyme makes any assessment of actual activity of this enzyme in the cells very difficult. In this work we made an attempt to estimate and compare the activity of calpain in human peripheral blood lymphocytes by assessing the levels of limited proteolysis of calpastatin in these cells by western blot, while at the same time the levels of calpain protein inside these cells was measured by flow cytometry. Our results indicate that it is possible to compare (semi-quantitatively the activities of calpain in peripheral blood CD4+ and CD19+ lymphocytes from various donors that way. Preliminary results showed that calpain activity is increased in the CD4+ T cells isolated from peripheral blood of rheumatoid arthritis patients as compared to control lymphocytes. Extremely high intrinsic activity of calpain was detected in chronic lymphocytic leukemia (CD19+ cells. All this confirms the detection of immunoreactive products of calpastatin as a good maker of endogenous calpain activity.

  15. Effect of guar crispbread with cereal products and leguminous seeds on blood glucose concentrations of diabetics.

    OpenAIRE

    Jenkins, D J; Wolever, T M; Taylor, R H; Barker, H M; Fielden, H; Jenkins, A. L.

    1980-01-01

    To compare the effect on blood glucose concentrations of guar incorporated into crispbreads with that of unprocessed high-fibre foods groups of four to six diabetics took a total of seven test breakfasts on separate days. By comparison with a breakfast of wholemeal bread and cheese, guar crispbread combined with bread reduced the area under the glucose response curve to 51% (p < 0.05); bread and soya beans reduced the area to 65% (p < 0.05); guar crispbread with soya beans to 25% (p < 0.002);...

  16. Scientific Opinion on the substantiation of a health claim related to OptiEFAX™ and maintenance of normal blood concentrations of triglycerides pursuant to Article 13(5) of Regulation (EC) No 1924/2006

    OpenAIRE

    EFSA Panel on Dietetic Products, Nutrition and Allergies (NDA)

    2012-01-01

    Following an application from Nutrilinks Sarl, submitted for authorisation of a health claim pursuant to Article 13(5) of Regulation (EC) No 1924/2006 via the Competent Authority of Belgium, the Panel on Dietetic Products, Nutrition and Allergies (NDA) was asked to deliver an opinion on the scientific substantiation of a health claim related to OptiEFAX™ and maintenance of normal blood concentrations of triglycerides. The food that is the subject of the health claim, OptiEF...

  17. Transfusion Related Acute Lung Injury (TRALI Caused by Red Blood Cell Transfusion Involving Residual Plasma Anti-HLA Antibodies: A report on two Cases and General Considerations

    Directory of Open Access Journals (Sweden)

    Olivier Garraud

    2005-01-01

    Full Text Available TRALI is considered a serious hazard among immune complications of blood transfusion and its occurrence is admitted to be globally underestimated. Each type of blood product is likely to cause TRALI. We report here on two consecutive observations of TRALI caused by red blood cell concentrates, in which anti-HLA class I and class II antibodies resulting from post-gravitational allo-immunization were evidenced in donors. HLA class I and II antigenic community between recipients and donors' husbands were found and strong reacting IgG antibodies directed at several of those common antigens were detected in the donors' serum. Both donors had more than 3 pregnancies, raising the issue of blood donor selection or of plasma reduction for cellular products.

  18. Some political issues related to future special nuclear materials production

    International Nuclear Information System (INIS)

    The Federal Government must take action to assure the future adequate supply of special nuclear materials for nuclear weapons. Existing statutes permit the construction of advanced defense production reactors and the reprocessing of commercial spent fuel for the production of special materials. Such actions would not only benefit the US nuclear reactor manufacturers, but also the US electric utilities that use nuclear reactors

  19. Milk kefir: composition, microbial cultures, biological activities, and related products.

    Science.gov (United States)

    Prado, Maria R; Blandón, Lina Marcela; Vandenberghe, Luciana P S; Rodrigues, Cristine; Castro, Guillermo R; Thomaz-Soccol, Vanete; Soccol, Carlos R

    2015-01-01

    In recent years, there has been a strong focus on beneficial foods with probiotic microorganisms and functional organic substances. In this context, there is an increasing interest in the commercial use of kefir, since it can be marketed as a natural beverage that has health promoting bacteria. There are numerous commercially available kefir based-products. Kefir may act as a matrix in the effective delivery of probiotic microorganisms in different types of products. Also, the presence of kefir's exopolysaccharides, known as kefiran, which has biological activity, certainly adds value to products. Kefiran can also be used separately in other food products and as a coating film for various food and pharmaceutical products. This article aims to update the information about kefir and its microbiological composition, biological activity of the kefir's microflora and the importance of kefiran as a beneficial health substance. PMID:26579086

  20. The relative labour productivity contribution of different age-skill categories for a developing economy

    OpenAIRE

    Gerhardus van Zyl

    2013-01-01

    Orientation: The article dealt with the estimation, computation and interpretation of the relative productivity contributions of different age-skill categories. Research purpose: The aim of the article was to estimate and compute, (1) relative productivity contributions and (2) relative productivity contribution–employee remuneration cost levels for different age-skill categories.Motivation for the study: The research was deemed necessary given the current debate on relative productivity le...

  1. Properdin-Mediated C5a Production Enhances Stable Binding of Platelets to Granulocytes in Human Whole Blood.

    Science.gov (United States)

    Blatt, Adam Z; Saggu, Gurpanna; Kulkarni, Koustubh V; Cortes, Claudio; Thurman, Joshua M; Ricklin, Daniel; Lambris, John D; Valenzuela, Jesus G; Ferreira, Viviana P

    2016-06-01

    Enhanced levels of platelet/granulocyte aggregates (PGAs) are found in patients suffering from many different inflammatory vascular diseases, and their formation in animal models of vascular disease is associated with increased thromboinflammation and worsened outcomes. The complement system, a part of the innate immune system, influences PGA formation, but the mechanisms for its effects are unknown. In this study, we have defined complement-mediated mechanisms that enhance PGA formation in human whole blood stimulated with thrombin receptor-activating peptide (TRAP) using ex vivo flow cytometry assays. We demonstrate that physiological properdin, a positive regulator of complement alternative pathway activity, increases PGA formation when added to TRAP-stimulated blood. All physiological properdin forms increase PGA formation, but properdin tetramers are the most efficient at increasing complement activity and PGA formation. Inhibition of endogenous properdin, either circulating in the blood or produced locally by leukocytes, impairs TRAP-mediated PGA formation to the same level as specific inhibition of either the alternative or classical pathway. Additionally, blocking the interaction of C5a with its cellular receptor prevents properdin-mediated increases in PGA formation. Adding either properdin tetramers or C5a to whole blood increases CD11b expression on granulocytes, and this increase is prevented by blockade of the C5a-C5a receptor axis. Finally, we demonstrate that the effects of properdin on PGA formation are tightly regulated by Factor H. Cumulatively, our data indicate that properdin enhances PGA formation via increased production of C5a, and that inhibition of properdin function has therapeutic potential to limit thromboinflammation in diseases characterized by increased PGA formation. PMID:27183616

  2. Relations between neuropsychological findings and lateral asymmetries of cerebral blood flow measured by SPECT in dementia of Alzheimer type

    International Nuclear Information System (INIS)

    We studied 16 right-handed patients clinically diagnosed as dementia of Alzheimer type (6 men, 10 women; aged 63-85, mean 72.8 years). The average duration of symptoms was 2.7 years. Dementia ranged from mild to moderately severe. None had clinical or laboratory evidence of cerebrovascular disease (Hachinski ischemic scores for all patients were 4 or below 4). All received the Wechsler Adult Intelligence Scale (WAIS), Mini-mental State Test (MMS) and Western Aphasia Battery (WAB, First Japanese edition, 1986). Regional cerebral blood flow (rCBF) was evaluated by single photon emission CT (SPECT) with 123I-N-isopropyl-p-iodoamphetamine (123I-IMP), using the Matsuda's quantitative method. Regional tracer uptake was measured in regions of interests (ROIs) over right and left frontal, temporal, parietal and occipital cortical regions; basal ganglia; and cerebellar hemispheres. The subjects were divided into three groups on the basis of lateral asymmetries in the temporal and parietal cortexes of rCBF (left< right, n=5; right< left, n=3; left=right, n=8). We decided that lateral asymmetry was present when rCBF for each ROI between left and right sides differs by more than 10%. General score (MMS, T-IQ) was not correlated with asymmetry of cerebral blood flow. Verbal IQ in patients with predominant hypoperfusion of left temporal and parietal lobe was significantly lower than other group, while performance IQ and WAB constructive scores were lower in those with right hemispheric hypoperfusion (p<0.05). We concluded that cerebral blood flow asymmetry by SPECT was related significantly to the deficit of language and constructive function in patients with dementia of Alzheimer type. Decreased rCBF in the left temporoparietal lobe was associated with language dysfunction, and that in the right hemisphere, with constructive dysfunction. (author)

  3. 21 CFR 640.1 - Whole Blood.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 7 2010-04-01 2010-04-01 false Whole Blood. 640.1 Section 640.1 Food and Drugs... STANDARDS FOR HUMAN BLOOD AND BLOOD PRODUCTS Whole Blood § 640.1 Whole Blood. The proper name of this product shall be Whole Blood. Whole Blood is defined as blood collected from human donors for...

  4. SPM analysis of cerebral blood flow changes related to age in patients with schizophrenia

    International Nuclear Information System (INIS)

    The goal of this study was to explore neural activities of schizophrenic patients related to current age including the age of onset and also we examined the correlation patterns between age and neural correlates. 21 patients with schizophrenia (female/male; 10/11, mean age; 36.1 11.2) participated in this study and all patients were evaluated by criteria of DSM-IV as a schizophrenia patient. Exclusion criteria included inability to give informed consent and a history of significant neurological illness. 99mTc-ECD brain perfusion SPECT images were obtained from 21 schizophrenic patients. Using SPM (statistical parametric mapping), all images were normalized to a SPECT template (MNI template) and then smoothed. We performed an ANCOVA analysis with current age (covariate variable) and the age of onset (nuisance variable). The correlation was analyzed into positive and negative patterns. Findings are reported as a Z scores with a significance threshold of p < 0.005 (uncorrected) and a minimum cluster size of 10. Significant difference was found between current age and premorbid age (p < 0.05). Cerebral regions that were positively correlated with current age were observed in the middle frontal gyrus and the superior frontal gyrus bilaterally, the left precentral gyrus (Brodmann's area; BA 9) and the right inferior frontal gyrus (BA 46). Also, in relation to negative correlations, there were many regions in the right posterior cingulate gyrus (BA 30) and the left cuneus (BA 30), middle temporal gyrus (BA 21) and superior temporal gyrus (BA 13, 39). Additionally, the occipital regions were found in the right cuneus (BA 18) including the right middle occipital gyrus (BA 18) and right lingual gyrus (BA 19). Age effect in schizophrenic patients was observed in the dorsolateral prefrontal cortex with positive correlation. We suggest that the dorsolateral prefrontal cortex activity which is important for cognitive task performance is related to an increase of patients age

  5. SPM analysis of cerebral blood flow changes related to age in patients with schizophrenia

    Energy Technology Data Exchange (ETDEWEB)

    Song, Ho Chun; Kim, Sung Wan; Lee, Byeong II; Min, Jung Joon; Bom, Hee Seung [Chonnam National University Medical School, Gwangju (Korea, Republic of)

    2007-07-01

    The goal of this study was to explore neural activities of schizophrenic patients related to current age including the age of onset and also we examined the correlation patterns between age and neural correlates. 21 patients with schizophrenia (female/male; 10/11, mean age; 36.1 11.2) participated in this study and all patients were evaluated by criteria of DSM-IV as a schizophrenia patient. Exclusion criteria included inability to give informed consent and a history of significant neurological illness. 99mTc-ECD brain perfusion SPECT images were obtained from 21 schizophrenic patients. Using SPM (statistical parametric mapping), all images were normalized to a SPECT template (MNI template) and then smoothed. We performed an ANCOVA analysis with current age (covariate variable) and the age of onset (nuisance variable). The correlation was analyzed into positive and negative patterns. Findings are reported as a Z scores with a significance threshold of p < 0.005 (uncorrected) and a minimum cluster size of 10. Significant difference was found between current age and premorbid age (p < 0.05). Cerebral regions that were positively correlated with current age were observed in the middle frontal gyrus and the superior frontal gyrus bilaterally, the left precentral gyrus (Brodmann's area; BA 9) and the right inferior frontal gyrus (BA 46). Also, in relation to negative correlations, there were many regions in the right posterior cingulate gyrus (BA 30) and the left cuneus (BA 30), middle temporal gyrus (BA 21) and superior temporal gyrus (BA 13, 39). Additionally, the occipital regions were found in the right cuneus (BA 18) including the right middle occipital gyrus (BA 18) and right lingual gyrus (BA 19). Age effect in schizophrenic patients was observed in the dorsolateral prefrontal cortex with positive correlation. We suggest that the dorsolateral prefrontal cortex activity which is important for cognitive task performance is related to an increase of patients

  6. 76 FR 25373 - Certain Polyimide Films, Products Containing Same, and Related Methods; Notice of Institution of...

    Science.gov (United States)

    2011-05-04

    ... COMMISSION Certain Polyimide Films, Products Containing Same, and Related Methods; Notice of Institution of... films, products containing same, and related methods by reason of infringement of certain claims of U.S... certain polyimide films, products containing same, and related methods that infringe one or more of...

  7. 78 FR 46367 - Certain Laundry and Household Cleaning Products and Related Packaging; Notice of Receipt of...

    Science.gov (United States)

    2013-07-31

    ... COMMISSION Certain Laundry and Household Cleaning Products and Related Packaging; Notice of Receipt of... Laundry and Household Cleaning Products and Related Packaging, DN 2969; the Commission is soliciting... importation of certain laundry and household cleaning products and related packaging. The complaint names...

  8. Related research of blood glucose, blood lipid and serum cystatin C and lower-extremity arterial disease in type 2 diabetic mellitus

    Institute of Scientific and Technical Information of China (English)

    Juan Zeng; Jie Ou; Yun-Mei He; Chun-Yu Cai

    2016-01-01

    Objective:To explore the relationship between levels of blood glucose, blood lipid and serum cystatin C and lower-extremity arterial disease in type 2 diabetic mellitus so as to provide the basis for the prevention and treatment of the disease.Methods:A total of 240 cases of patients with type 2 diabetic mellitus receiving physical examinations in our hospital from March 2014 to March 2015 were selected and divided into three groups. The control group was consisted of type 2 diabetes patients without lower-extremity arterial disease, the observation group 1 included type 2 diabetes patients with mild or moderate lower-extremity arterial disease, and the observation group 2 was formed by type 2 diabetes patients with severe lower-extremity arterial disease. Each group contained 80 cases. Then, the heights, weights, waist and hip circumferences of patients in the three groups were determined and the body mass index (BMI) and waist to hip ratio (WHR) were calculated; the fasting blood glucose (FBG), fasting insulin (FINS), insulin resistance index (HOMA-IR), 2 h postprandial blood glucose (2 h PG) and glycosylated hemoglobin (HBA1c) in two groups were detected; and the levels of serum total cholesterol(TC), triglyceride (TG), low density lipoprotein cholesterol (LDL-C), high density lipoprotein cholesterol (HDL-C), apolipoprotein A1 (apoA1), apolipoprotein B (apoB) and cystatin C (CysC) in two groups were also tested.Results:Compared with the control group, the WHR, FBG, HOMA-IR, 2 h PG, HBA1c, LDL-C, apoB and CysC all increased significantly, and the differences were statistically significant. Besides, there were statistically significances existing between the observation groups 1 and 2. What’s more, the serum levels of TC, HDL-C, TG and apoA1 in the observation groups were not significantly different from those of the control group.Conclusions: There is a close relationship between blood glucose, blood lipid, serum cystatin C level and lower-extremity arterial

  9. Methods and materials relating to IMPDH and GMP production

    Science.gov (United States)

    Collart, Frank R.; Huberman, Eliezer

    1997-01-01

    Disclosed are purified and isolated DNA sequences encoding eukaryotic proteins possessing biological properties of inosine 5'-monophosphate dehydrogenase ("IMPDH"). Illustratively, mammalian (e.g., human) IMPDH-encoding DNA sequences are useful in transformation or transfection of host cells for the large scale recombinant production of the enzymatically active expression products and/or products (e.g., GMP) resulting from IMPDH catalyzed synthesis in cells. Vectors including IMPDH-encoding DNA sequences are useful in gene amplification procedures. Recombinant proteins and synthetic peptides provided by the invention are useful as immunological reagents and in the preparation of antibodies (including polyclonal and monoclonal antibodies) for quantitative detection of IMPDH.

  10. Production of cytokine and chemokines by human mononuclear cells and whole blood cells after infection with Trypanosoma cruzi

    Directory of Open Access Journals (Sweden)

    Karine Rezende-Oliveira

    2012-02-01

    Full Text Available INTRODUCTION: The innate immune response is the first mechanism of protection against Trypanosoma cruzi, and the interaction of inflammatory cells with parasite molecules may activate this response and modulate the adaptive immune system. This study aimed to analyze the levels of cytokines and chemokines synthesized by the whole blood cells (WBC and peripheral blood mononuclear cells (PBMC of individuals seronegative for Chagas disease after interaction with live T. cruzi trypomastigotes. METHODS: IL-12, IL-10, TNF-α, TGF-β, CCL-5, CCL-2, CCL-3, and CXCL-9 were measured by ELISA. Nitrite was determined by the Griess method. RESULTS: IL-10 was produced at high levels by WBC compared with PBMC, even after incubation with live trypomastigotes. Production of TNF-α by both PBMC and WBC was significantly higher after stimulation with trypomastigotes. Only PBMC produced significantly higher levels of IL-12 after parasite stimulation. Stimulation of cultures with trypomastigotes induced an increase of CXCL-9 levels produced by WBC. Nitrite levels produced by PBMC increased after the addition of parasites to the culture. CONCLUSIONS: Surface molecules of T. cruzi may induce the production of cytokines and chemokines by cells of the innate immune system through the activation of specific receptors not evaluated in this experiment. The ability to induce IL-12 and TNF-α contributes to shift the adaptive response towards a Th1 profile.

  11. Morphometric Evaluation of the Blood Pressure Related Organs in Nili-Ravi Buffalo (Bubalus Bubalis With Advancing Age

    Directory of Open Access Journals (Sweden)

    R. Hussain

    2010-02-01

    Full Text Available Forty clinically healthy buffaloes of either sex were divided into two age-groups of equal size i.e., young (12-42 months and adult (48-96 months. Histological sections were prepared by routine method and stained by H&E. AutoCAD® image analysis software and ocular micrometer were used for the morphometric evaluations. Studies revealed that the adrenal cortex of buffalo was divided into four zones, zona glomerulosa, zona intermedia, zona fasciculate and zona reticularis. The zona fasciculata was recorded as the widest and zona reticularis narrowest zone of the adrenal cortex. The volume of cardiomyocytic nuclei (μm3, volume of renal corpuscles (μm3×103, volume of nuclei of cells of zona glomerulosa (μm3, thickness of the zona glomerulosa, zona intermedia and zona fasciculata of adrenal cortex in adult buffaloes were significantly (P<0.01 higher as compared to their younger counterparts. It is conceivable that the development of blood related parameters showed an increase parallel to the advancing age in order to adjust with the increasing blood pressure due to physiological development process in buffalo.

  12. Stress associated gene expression in blood cells is related to outcome in radiotherapy treated head and neck cancer patients

    International Nuclear Information System (INIS)

    We previously observed that a radiotherapy-induced biochemical response in plasma was associated with favourable outcome in head and neck squamous carcinoma cancer (HNSCC) patients. The aim of the present study was to compare stress associated blood cell gene expression between two sub-groups of HNSCC patients with different biochemical responses to radiotherapy. Out of 87 patients (histologically verified), 10 biochemical ‘responders’ having a high relative increase in plasma oxidative damage and a concomitant decrease in plasma antioxidants during radiotherapy and 10 ‘poor-responders’ were selected for gene-expression analysis and compared using gene set enrichment analysis. There was a significant induction of stress-relevant gene-sets in the responders following radiotherapy compared to the poor-responders. The relevance of the involvement of similar stress associated gene expression for HNSCC cancer and radioresistance was verified using two publicly available data sets of 42 HNSCC cases and 14 controls (GEO GSE6791), and radiation resistant and radiation sensitive HNSCC xenografts (E-GEOD-9716). Radiotherapy induces a systemic stress response, as revealed by induction of stress relevant gene expression in blood cells, which is associated to favourable outcome in a cohort of 87 HNSCC patients. Whether these changes in gene expression reflects a systemic effect or are biomarkers of the tumour micro-environmental status needs further study. Raw data are available at ArrayExpress under accession number E-MEXP-2460

  13. Stress associated gene expression in blood cells is related to outcome in radiotherapy treated head and neck cancer patients

    Directory of Open Access Journals (Sweden)

    Bøhn Siv K

    2012-09-01

    Full Text Available Abstract Background We previously observed that a radiotherapy-induced biochemical response in plasma was associated with favourable outcome in head and neck squamous carcinoma cancer (HNSCC patients. The aim of the present study was to compare stress associated blood cell gene expression between two sub-groups of HNSCC patients with different biochemical responses to radiotherapy. Methods Out of 87 patients (histologically verified, 10 biochemical ‘responders’ having a high relative increase in plasma oxidative damage and a concomitant decrease in plasma antioxidants during radiotherapy and 10 ‘poor-responders’ were selected for gene-expression analysis and compared using gene set enrichment analysis. Results There was a significant induction of stress-relevant gene-sets in the responders following radiotherapy compared to the poor-responders. The relevance of the involvement of similar stress associated gene expression for HNSCC cancer and radioresistance was verified using two publicly available data sets of 42 HNSCC cases and 14 controls (GEO GSE6791, and radiation resistant and radiation sensitive HNSCC xenografts (E-GEOD-9716. Conclusions Radiotherapy induces a systemic stress response, as revealed by induction of stress relevant gene expression in blood cells, which is associated to favourable outcome in a cohort of 87 HNSCC patients. Whether these changes in gene expression reflects a systemic effect or are biomarkers of the tumour micro-environmental status needs further study. Trial registration Raw data are available at ArrayExpress under accession number E-MEXP-2460.

  14. Genotypic variation and slime production among blood and catheter isolates of Candida parapsilosis.

    OpenAIRE

    Branchini, M L; Pfaller, M A; Rhine-Chalberg, J; Frempong, T; Isenberg, H D

    1994-01-01

    Candida parapsilosis is an important nosocomial pathogen that can proliferate in high concentrations of glucose and form biofilms on prosthetic materials. We investigated the genotypic diversity and slime production among 31 isolates of C. parapsilosis from individual patients with bloodstream or catheter infections. DNA subtyping was performed by using electrophoretic karyotyping plus restriction endonuclease analysis with BssHII followed by pulsed-field gel electrophoresis. Slime production...

  15. Determination of blood ET-1 and related cytokines in pediatric patients with bronchial asthma

    International Nuclear Information System (INIS)

    Objective: To explore the role of endothelin and related cytokines (IL-5, IL-6, IL-8) in the development of bronchial asthma in pediatric patients. Methods: Plasma ET-1 (with RIA) and serum IL-5 (with ELISA), IL-6, IL-8 (with RIA) levels were measured in 42 pediatric patients with bronchial asthma and 30 controls. Results: The plasma ET-1 levels in the patients during acute attack (n=22) were significantly higher than those in patients during remission (n=20) as well as in controls (P0.05). Levels of ET-1 were positively correlated with those of IL-5 (during attack, r=0.560, P<0.01; during remission, r=0.435, P<0.01). Conclusion: ET and the cytokines IL-5, IL-6, IL-8 participated in the pathogenesis of bronchial asthma through different mechanisms and dynamic determination of which could reflect the progression of the disease. (authors)

  16. Production Technologies And Exchange Relations In International Industrial Markets

    OpenAIRE

    Hallén, L; Johanson, J.; Seyed Mohamed, N

    1986-01-01

    Based on interviews with marketers in British, German, and Swedish industrial firms, 237 relationships to customers are investigated with regard to the latent factors adaptation and contact intensity. A Lisrel model is developed, which shows that these factors are present in the total material as well as in two of the three specified technological categories, i.e. in relationships to customers with unit and mass production but not in relationships to customers with process production. Further...

  17. Hyaluronidase production in Streptococcus milleri in relation to infection.

    OpenAIRE

    Unsworth, P F

    1989-01-01

    One hundred and seven (41%) of 262 isolates of Streptococcus milleri, from human sources, produced hyaluronidase. Hyaluronidase production was commoner in beta haemolytic isolates 32 of 39 (82%), many of which were of Lancefield group F. But hyaluronidase was also found in alpha and non-haemolytic isolates, and in groups A, C, G, and non-groupable isolates. There was a strong association between hyaluronidase production and isolation from known internal abscesses (48/58, 83%) compared with is...

  18. Milk kefir: composition, microbial cultures, biological activities, and related products

    OpenAIRE

    Prado, Maria R.; Blandón, Lina Marcela; Vandenberghe, Luciana P. S.; Rodrigues, Cristine; Castro, Guillermo R; Thomaz-Soccol, Vanete; Carlos R Soccol

    2015-01-01

    In recent years, there has been a strong focus on beneficial foods with probiotic microorganisms and functional organic substances. In this context, there is an increasing interest in the commercial use of kefir, since it can be marketed as a natural beverage that has health promoting bacteria. There are numerous commercially available kefir based-products. Kefir may act as a matrix in the effective delivery of probiotic microorganisms in different types of products. Also, the presence of kef...

  19. Authentication of organic tomatoes, wheat, and related products

    OpenAIRE

    Novotná, Hana; Schulzova, Vera; Krtkova, Veronika; Hajslova, Jana

    2013-01-01

    There is an increasing consumer demand for food cultivated by organic farming practices. Because of higher price of these products, there is a need for development and validation of analytical methods, which allow discrimination between organic and conventional food products. This contribution concerns with one of the novel methods of food analysis, the metabolomic fingerprinting/profiling by the ambient mass spectrometry employing Direct Analysis in Real Time (DART) ion source coupled with O...

  20. GENOTYPES OF FIVE BLOOD PROTEINS’ POLYMORPHISM IN VARIOUS PRODUCTION AGE OF DAIRY COWS

    Directory of Open Access Journals (Sweden)

    J ŽITNÝ

    2001-09-01

    Full Text Available In this work were analysed samples of blood from 314 dairy cows Slovak spotted breed by the method of starch gel electrophoresis. The aim was to identify the biochemical polymorphic markers in blood plasma and in extract of erythrocytes. The polymorphism of hemoglobin (AA+AB+BB, amylasa (AA+AB+BB, transferrin (AA+AD1+AD2+AE+D1D1+D1E, albumin (AA+AB+BB and postalbumin (AA+AB+AC+BB+BC+CC were proved. File of dairy cows was divided to fi ve groups by age. The fi rst group create of dairy cows in age 25-48 months, second 49-72 months, third 73-96 months, fourth 97-120 months and the last over 121 months. According to evaluation of heterozygotes genotypes were detect decrease into of AB genotype in polymorphism of hemoglobin from primary 46.8 % (25-48 month to 11.1 % in the last age group of dairy cows up 121-th months. Heterozygotic combinations in transferrin locus (AD1+AD2+AE+D1E genotypes culminated from the beginning 14.2 % to 38.9 % at the fi rst group and last one. The postalbumin polymorphism had the high rate of heterozygotes because of AB+AC+BC genotypes. Rate of heterozygous genotypes AB in the polymorphism system amylasa variance between 15.4 - 23.7 %. Heterozygotic genotypes AB in albumin locus was according to our analyse at dairy cows in the fi rst evaluated of age group 52.9 %, second one only in fi fth groups at intervals 71.8 - 75.0 %. Among tested heterozygotes (AB genotype and homozygotes (AA+BB genotypes we found the signifi cant differences only in albumin locus 2,18+ (+P≤0,05.

  1. Reference values of central blood pressure and pulse wave velocity in relation with 24 hours ambulatory blood pressure monitoring in Belgian healthy young subjects

    OpenAIRE

    Saint-Remy, Annie; Krzesinski, Jean-Marie

    2010-01-01

    The present study aimed to define reference values of central blood pressure (cBP) and Pulse Wave Velocity (PWV) together with 24H ABPM in healthy normotensive young adults before starring a follow-up of their CV profile modifications over time. Peer reviewed

  2. Reference values of central blood pressure and pulse wave velocity in relations with 24 hours ambulatory blood pressure monitoring in Belgian normotensive young subjects

    OpenAIRE

    Saint-Remy, Annie; Krzesinski, Jean-Marie

    2010-01-01

    The present study aimed to define reference values of central blood pressure (cBP) and Pulse Wave Velocity (PWV) together with 24H APPM in healththy normotensive young adults before starting a follow-up of their CV profile modifications over time. Peer reviewed

  3. Extraction of mRNA from coagulated horse blood and analysis of inflammation-related cytokine responses to coagulation

    DEFF Research Database (Denmark)

    Bovbjerg, Kirsten Katrine Lindegaard; Heegaard, Peter M. H.; Skovgaard, Kerstin

    2010-01-01

    Coagulated blood is a rich source of mRNA that allows the study of the regulation of expression of cytokine and other genes. However, while several methods are available for isolation of RNA from whole blood and tissues, protocols for purification of mRNA from clotted blood are not generally...

  4. Blood Clots

    Science.gov (United States)

    ... Index A-Z Blood Clots Blood clots are semi-solid masses of blood that can be stationary (thrombosis) ... treated? What are blood clots? Blood clots are semi-solid masses of blood. Normally, blood flows freely through ...

  5. Age and dose related alteration of in vitro mixed lymphocyte culture response of blood lymphocytes from A-bomb survivors

    International Nuclear Information System (INIS)

    The responsiveness of peripheral blood lymphocytes to allogenic antigens in mixed lymphocyte culture (MLC) was measured in 139 atomic-bomb survivors. The study revealed a significant decrease in MLC response with increasing dose of previous radiation exposure. This decline was marked in the survivors who were older than 15 at the time of the bomb (ATB). The results suggest a possible relationship between the recovery of T-cell-related function and the thymic function which processes mature T cells for the immune system. Thus it may be that in the advanced age ATB group, the thymus function had started to involute, allowing less recovery of T-cell function compared to young survivors who had adequate processing T-cell activity

  6. Case report of transfusion-related acute lung injury in a pediatric spine surgery patient transfused leukoreduced red blood cells.

    Science.gov (United States)

    Cudilo, Elizabeth M; Varughese, Anna M; Mahmoud, Mohamed; Carey, Patricia M; Subramanyam, Rajeev

    2015-12-01

    Despite leukoreduced red blood cells (LR-RBCs) reducing the risk of transfusion-related acute lung injury (TRALI), we present a case of a 16-year-old female with kyphosis who received a transfusion of one unit of LR-RBCs, which lead to life-threatening, intraoperative TRALI. The clinical presentation included pulmonary edema, severe postoperative lactic acidosis, left ventricular dysfunction, increased creatine phosphokinase, fatty infiltration of the liver, and hemodynamic instability requiring inotropic support. This presentation is not the classic description of TRALI. Our patient improved with supportive treatment and was successfully extubated on postoperative day 4. TRALI work-up revealed antibody formation to HLA A2, A68, B44, and DQA 5 for the LR-RBCs unit administered. PMID:26126598

  7. Optimizing conditions of large scale production of monoclonal antibodies for determination of blood group

    International Nuclear Information System (INIS)

    Conditions for large scale production of monoclonal antibodies (monoAB) on mice hybrids were determined: irradiation dose, amount of introduced hybridoma cells, possible substitution of imported pristane for Soviet-produced vaseline oil or a mixture thereof. DMSO did not exert toxic action neither on the cell itself, nor on the development of ascytic tumor in mice after thawing of cryopreserved cells. This enabled to abstain from washing of DMSO off the introduced cells. A simple method for enhancing the monoAB yield - acquisition of the washing-out solution for increased efficiency of large scale production - was developed and tested

  8. The National Heart, Lung, and Blood Institute retrovirus epidemiology donor studies (Retrovirus Epidemiology Donor Study and Retrovirus Epidemiology Donor Study-II): twenty years of research to advance blood product safety and availability.

    Science.gov (United States)

    Kleinman, Steven; King, Melissa R; Busch, Michael P; Murphy, Edward L; Glynn, Simone A

    2012-10-01

    The Retrovirus Epidemiology Donor Study (REDS), conducted from 1989 to 2001, and the REDS-II, conducted from 2004 to 2012, were National Heart, Lung, and Blood Institute-funded, multicenter programs focused on improving blood safety and availability in the United States. The REDS-II also included international study sites in Brazil and China. The 3 major research domains of REDS/REDS-II have been infectious disease risk evaluation, blood donation availability, and blood donor characterization. Both programs have made significant contributions to transfusion medicine research methodology by the use of mathematical modeling, large-scale donor surveys, innovative methods of repository sample storage, and establishing an infrastructure that responded to potential emerging blood safety threats such as xenotropic murine leukemia virus-related virus. Blood safety studies have included protocols evaluating epidemiologic and/or laboratory aspects of human immunodeficiency virus, human T-lymphotropic virus 1/2, hepatitis C virus, hepatitis B virus, West Nile virus, cytomegalovirus, human herpesvirus 8, parvovirus B19, malaria, Creutzfeldt-Jakob disease, influenza, and Trypanosoma cruzi infections. Other analyses have characterized blood donor demographics, motivations to donate, factors influencing donor return, behavioral risk factors, donors' perception of the blood donation screening process, and aspects of donor deferral. In REDS-II, 2 large-scale blood donor protocols examined iron deficiency in donors and the prevalence of leukocyte antibodies. This review describes the major study results from over 150 peer-reviewed articles published by these 2 REDS programs. In 2011, a new 7-year program, the Recipient Epidemiology and Donor Evaluation Study-III, was launched. The Recipient Epidemiology and Donor Evaluation Study-III expands beyond donor-based research to include studies of blood transfusion recipients in the hospital setting and adds a third country, South Africa

  9. International market segmentation based on consumer-product relations

    NARCIS (Netherlands)

    Ter Hofstede, F; Steenkamp, JBEM; Wedel, M

    1999-01-01

    With increasing competition in the global marketplace, international segmentation has become an ever more important issue in developing, positioning, and selling products across national borders. The authors propose a methodology to identify cross-national market segments, based on means-end chain t

  10. Anticancer agent-based marine natural products and related compounds.

    Science.gov (United States)

    Chen, Jian-Wei; Wu, Qi-Hao; Rowley, David C; Al-Kareef, Ammar M Q; Wang, Hong

    2015-01-01

    Marine natural products constitute a huge reservoir of anticancer agents. Consequently during the past decades, several marine anticancer compounds have been isolated, identified, and approved for anticancer treatment or are under trials. In this article the sources, structure, bioactivities, mode of actions, and analogs of some promising marine and derived anticancer compounds have been discussed. PMID:25559315

  11. Leader-follower interactions : Relations with OCB and sales productivity

    NARCIS (Netherlands)

    Zacher, Hannes; Jimmieson, Nerina L.

    2013-01-01

    Purpose Based on substitutes for leadership theory, the aim of this study is to examine followers' learning goal orientation as a moderator of relationships among transformational leadership, organizational citizenship behavior (OCB) and sales productivity. Design/methodology/approach Data came from

  12. EFSA NDA Panel (EFSA Panel on Dietetic Products, Nutrition and Allergies), 2013. Scientific Opinion on the substantiation of a health claim related to a combination of red spinach, green spinach, red chicory, green chicory, green leaf chard, red leaf chard, red Swiss chard, golden Swiss chard and white Swiss chard and maintenance of normal blood LDL-cholesterol concentration pursuant to Article 13(5) of Regulation (EC) No 1924/2006

    OpenAIRE

    Tetens, Inge

    2013-01-01

    Following an application from Italsur s.r.l., submitted pursuant to Article 13(5) of Regulation (EC) No 1924/2006 via the Competent Authority of Italy, the Panel on Dietetic Products, Nutrition and Allergies (NDA) was asked to deliver an opinion on the scientific substantiation of a health claim related to a combination of red spinach, green spinach, red chicory, green chicory, green leaf chard, red leaf chard, red Swiss chard, golden Swiss chard and white Swiss chard and maintenance of norma...

  13. Relative toxicity of pyrolysis products of some foams and fabrics

    Science.gov (United States)

    Hilado, C. J.

    1976-01-01

    A limited number of foams and fabrics was evaluated in the course of developing test procedures for determining the relative toxicity of materials. The principal variable studied, heating rate, did not affect the relative ranking of the materials tested. Two pyrolysis test procedures using the same basic approach but employing different sample weights, chamber volumes, laboratory animals, heating rates, and upper temperature limits, resulted in identical rankings of relative toxicity. The data obtained show that modification of conventional flexible polyurethane foams with flame retardants to comply with California upholstered furniture flammability regulations seems to consistently reduce toxicity under pyrolysis conditions.

  14. Alzheimer’s disease: are blood and brain markers related? A systematic review:Blood and Brain Markers of Alzheimer’s Disease

    OpenAIRE

    Khan, Ali; Dobson, Richard James Butler; Sattlecker, Martina; Kiddle, Steven John

    2016-01-01

    Objective: Peripheral protein biomarkers of Alzheimer’s disease (AD) may helpidentify novel treatment avenues by allowing early diagnosis, recruitment toclinical trials, and treatment initiation. The purpose of this review was to deter-mine which proteins have been found to be differentially expressed in the ADbrain and whether these proteins are also found within the blood of ADpatients. Methods: A two-stage approach was conducted. The first stageinvolved conducting a systematic search to ide...

  15. Volvatellin, caulerpenyne-related product fromt he sacoglossan Volvatella sp.

    Digital Repository Service at National Institute of Oceanography (India)

    Fontana, A.; Ciavatta, M.L.; Mollo, E.; Naik, C.G.; Wahidullah, S.; DeSouza, L.; Cimino, G.

    Volvatellin (4) is a highly unstable terpene isolated from the extracts of the Indian opisthobranch mollusk Volvatella sp. The structure and the relative stereochemistry of 4 were determined by NMR methods. The paper also describes a hypothetical...

  16. "Eating well": the politics of production and relational aesthetics

    OpenAIRE

    Walden, Jenny

    2009-01-01

    Relational Aesthetics” has emerged as a phenomenon in art critical discourse through the work of Nicholas Bourriard and his categorisation of certain modes of creating art works, involving the participation of the ‘viewer’ in distinctive ways, such that a new ethical and political relation subtends, displacing former political ‘boundary markers’ between object and audience. The chapter will discuss two significant ways in which ‘the politics of production’ in connection with arts works consi...

  17. Storage time effect on blood diet for tsetse mass production in sterile insect technique

    OpenAIRE

    M. Byamungu, S. Matembo, K. Benedict & G. Mashenga

    2011-01-01

    Background & objectives: Different methods have been developed for tsetse and trypanosomiasis control oreradication but all these have their specific advantages and limitations. However, a combination of methodscould be used as part of an Area-wide Integrated Pest Management (AWPM). The sterile insect technique(SIT) as a technique of choice for eradication of tsetse flies requires mass production of flies in the laboratories.Since tsetses are exclusively haemotophogous insects, a quality bloo...

  18. Comparing the effect of whole body massage by a specialist nurse and patients’ relatives on blood cortisol level in coronary patients

    OpenAIRE

    Adib-Hajbaghery, Mohsen; Rajabi-Beheshtabad, Rahman; Ardjmand, Abolfazl

    2015-01-01

    BACKGROUND Cardiovascular diseases such as acute coronary syndrome and myocardial infarction are often accompanied by severe anxiety over the likelihood of death. Cortisol has been known as a stress hormone. However, there are controversies about the effect of massage therapy on blood cortisol level. Furthermore, no study is available on the difference between massage applied by a nurse specialist or by patients’ relatives on blood cortisol level. This study was aimed to compare the effect of...

  19. Unemployment persistence and the unemployment-productivity relation

    OpenAIRE

    Snower, Dennis J.

    1994-01-01

    This paper explores the interrelation between the degree of unemployment persistence and the unemployment-productivity trade-off. The analysis suggests that the more effective are structural labour market policies (designed to change labour market institutions and laws, such as job security legislation) in making wages and prices more responsive to current unemployment and making unemployment less persistent, the less effective will be the growth-promoting supply-side policies (such as traini...

  20. Product-related Environmental Performance Indicators: a systematic literature review

    DEFF Research Database (Denmark)

    Issa, Isabela I.; Antelmi Pigosso, Daniela Cristina; McAloone, Tim C.; Rozenfeld, Henrique

    2013-01-01

    indicators (EPIs) during PDP is often cited as a successful factor for effective ecodesign implementation, since it enables the monitoring towards the accomplishment of environmental performance improvement goals. However, companies still face difficulties in the selection and application of EPIs - a more......Ecodesign is a proactive environmental management approach employed in the product development process (PDP) which aims to minimize the environmental impacts caused during products’ life-cycle, improving its environmental performance. The establishment of measurable environmental performance...

  1. Factors Related to Low Research Productivity at Higher Education Level

    OpenAIRE

    Muhammad Zafar Iqbal; Azhar Mahmood

    2011-01-01

    Research is vital and necessary part of modern university education; universities are producers of new knowledge. Role of universities is different from the 19th century; demands of the 21st century are enormously higher. The purpose of study was to find out the causes of low research productivity at university level. Population of the study was faculty members working at University. Sample consisting of 232 male and female faculty members was selected through the stratified sampling techniqu...

  2. Development of functional food products in relation to obesity

    Directory of Open Access Journals (Sweden)

    Monika Choudhary

    2012-06-01

    Full Text Available Abstract:The development of new eating habits, as well as actual trends in production and consumption,has a health, environmental and social impact. The entire world is fighting diseases characteristic of the modern age such as obesity, osteoporosis, cancer, diabetes, allergies, and dental problems. With a global increase in the prevalence of obesity, both nutrition and exercise play key roles in its prevention and treatment. Natural product (nutraceutical interventions are currently being investigated on a large-scale basis as potential treatments for obesity and weight management. With advancing nutritional sciences, several nutrients such as; low-glycemic-indexcarbohydrates, 5-hydroxytryptophan, green tea extract, and chromium have been shown topromote weight loss. The first two nutrients decrease appetite, green tea increases the 24 hrenergy expenditure, and chromium promotes the composition of the weight lost to be fat rather than lean tissue. These have been assembled, in efficacious doses, into a new functional food product and described in this review. Each component has already been shown to promote weight loss independently in clinical trials.

  3. Production and properties of monoclonal antibodies to human blood coagulation factor VII and factor VIIa

    International Nuclear Information System (INIS)

    Human factor VII is a trace vitamin K-dependent protein that circulates in blood as a single-chain precursor to a serine protease. Upon activation, two-chain factor VIIa activates factor x in the presence of tissue factor and calcium. Purified preparations of single-chain (SC) human factor VII and two-chain (TC) factor VIIa were utilized to immunize Balb/c mice. Spleen cells from these immunized mice were fused to a non-secreting NS-1 derivative of X63-Ag8 myeloma cells and grown in selective medium. Analysis of culture supernatants by EIA revealed several hybridomas that were secreting IgG specific for Sc-factor VII and TC-factor VIIa. In addition, several hybridomas secreted IgG that reacted equally well with factor VII and factor VIIa. One of the latter McAb (A-29) reacted with the heavy chain of factor VIIa and the intact factor VII molecule equally as judged by Western blotting. A-29 was produced in ascites fluid, purified and coupled to activated CH-Sepharose. Application of one liter of normal human plasma to 10 ml of this immunoadsorbent column, elution of factor VII and subsequent Western blot using 125I-rabbit anti-human factor VII indicated a single species of factor VII(M/sub r/ = 50 KDa) in normal plasma. These specific factor VII/VIIa McAbs may prove useful in the analysis of these factors, and in the separation of SC-factor VII from TC-factor VIIa

  4. Proliferation and TH1/TH2 Cytokine Production in Human Peripheral Blood Mononuclear Cells after Treatment with Cypermethrin and Mancozeb In Vitro

    Directory of Open Access Journals (Sweden)

    Rajesh Mandarapu

    2014-01-01

    Full Text Available In recent times, human cell-based assays are gaining attention in assessments of immunomodulatory effects of chemicals. In the study here, the possible effects of cypermethrin and mancozeb on lymphocyte proliferation and proinflammatory (tumor necrosis factor (TNF- α and immunoregulatory cytokine (interferon- (IFN- γ, interleukins (IL 2, 4, 6, and 10 formation in vitro were investigated. Human peripheral blood mononuclear cells (PBMC were isolated and exposed for 6 hr to noncytotoxic doses (0.45–30 µM of cypermethrin or mancozeb in the presence of activating rat S9 fraction. Cultures were then further incubated for 48 or 72 hr in fresh medium containing phytohemagglutinin (10 µg/mL to assess, respectively, effects on cell proliferation (BrdU-ELISA method and cytokine formation (flow cytometric bead immunoassays. Mancozeb induced dose-dependent increases in lymphocyte proliferation, inhibition of production of TNFα and the TH2 cytokines IL-6 and IL-10, and an increase in IFNγ (TH1 cytokine production (at least 2-fold compared to control; mancozeb also induced inhibition of IL-4 (TH2 and stimulated IL-2 (TH1 production, albeit only in dose-related manners for each. In contrast, cypermethrin exposure did not cause significant effects on proliferation or cytokine profiles. Further studies are needed to better understand the functional significance of our in vitro findings.

  5. Relative toxicity of pyrolysis products of some aircraft seat materials

    Science.gov (United States)

    Hilado, C. J.; Marcussen, W. H.; Furst, A.; Kourtides, D. A.; Parker, J. A.; Fewell, L. L.

    1977-01-01

    Eighteen samples of aircraft seat materials were evaluated for relative toxicity using the USF/NASA toxicity screening test method. Nine samples were upholstery fabrics and nine samples were cushioning foams. Under these particular conditions of test, the aromatic phenolic and aromatic polyamide fabrics exhibited less toxicity than the samples of wool and wool/nylon fabrics, and the samples of neoprene foams exhibited less toxicity than the samples of polyurethane foams. These relative toxicity rankings were obtained using both apparent lethal concentration for 50 percent of the test animals (ALC50), and time to death (Td) at a fixed weight of material.

  6. New insights into the effects on blood pressure of diets low in salt and high in fruits and vegetables and low-fat dairy products

    OpenAIRE

    Sacks Frank M; Vollmer William M; Svetkey Laura P

    2001-01-01

    Abstract Results from the recent Dietary Approaches to Stop Hypertension (DASH)-Sodium trial provide the latest evidence concerning the effects of dietary patterns and sodium intake on blood pressure. Participants ate either the DASH diet (high in fruits, vegetables and low-fat dairy products, and reduced in saturated and total fat) or a typical US diet. Within each diet arm, participants ate higher, intermediate, and lower sodium levels, each for 30 days. The results indicated lower blood pr...

  7. The Relation of Plant Design to Product Cost

    International Nuclear Information System (INIS)

    The cost of medical supplies varies enormously, from a few cents per unit to a figure many times this. In the latter case the capital cost of medical supplies held up in the irradiation cell can vary considerably and may be of the order of $100 000 or more. To reduce the cost of capital invested in the irradiation plant and the stock of product in the plant requires a special design approach resulting in a plant design that is unusual. The plant resulting is that employed by ETHICON Limited in Edinburgh, Scotland. (author)

  8. Health care professionals’ knowledge, attitudes and practices relating to umbilical cord blood banking and donation: an integrative review

    OpenAIRE

    Peberdy, Lisa; Young, Jeanine; Kearney, Lauren

    2016-01-01

    Background Collection and storage of an infant’s cord blood at birth is an option available to many new parents. Antenatal health care providers have an important role in providing non-biased and evidence based information to expectant parents about cord blood and tissue banking options. The aim of this paper was to identify and review studies of health care professionals’ knowledge, attitudes and practices concerning cord blood banking and the sources by which healthcare professionals obtain...

  9. ‘Adaptive’ Psychosocial Factors in Relation to Home Blood Pressure: A Study in the General Population of Southern Netherlands

    OpenAIRE

    Nyklicek, I.; Vingerhoets, A.J.J.M.

    2009-01-01

    Background Evidence is accumulating for an association between psychosocial stress and elevated blood pressure. However, studies focusing on adaptive psychosocial factors are scarce. Purpose We examined the association between putatively adaptive psychosocial factors and home blood pressure in a population study in the Netherlands. Method Resting blood pressure was measured of 985 female and 777 male participants between 20 and 55 years of age in their home setting. Questionnaires assessing p...

  10. Blood glucose and meal patterns in time-blinded males, after aspartame, carbohydrate, and fat consumption, in relation to sweetness perception.

    Science.gov (United States)

    Melanson, K J; Westerterp-Plantenga, M S; Campfield, L A; Saris, W H

    1999-12-01

    In a study of the impact of aspartame, fat, and carbohydrate on appetite, we monitored blood glucose continuously for 431 (SE 16) min. Ten healthy males (19-31 years) participated in three time-blinded visits. As blood glucose was monitored, appetite ratings were scored at randomized times. On the first meal initiation, volunteers consumed one of three isovolumetric drinks (aspartame, 1 MJ simple carbohydrate, and 1 MJ high-fat; randomized order). High-fat and high-carbohydrate foods were available ad libitum subsequently. Blood glucose patterns following the carbohydrate drink (+1.78 (SE 0.28) mmol/l in 38 (SE 3) min) and high-fat drink (+0.83 (SE 0.28) mmol/l in 49 (SE 6) min) were predictive of the next intermeal interval (R 0.64 and R 0.97 respectively). Aspartame ingestion was followed by blood glucose declines (40% of subjects), increases (20%), or stability (40%). These patterns were related to the volunteers' perception of sweetness of the drink (R 0.81, P = 0.014), and were predictive of subsequent intakes (R -0.71, P = 0.048). For all drinks combined, declines in blood glucose and meal initiation were significantly associated (chi 2 16.8, P blood glucose responses and intermeal intervals correlated significantly (R 0.715, P = 0.0001), and sweetness perception correlated negatively with hunger suppression (R -0.471, P = 0.015). Effects of fat, carbohydrate, and aspartame on meal initiation, meal size, and intermeal interval relate to blood glucose patterns. Varied blood glucose responses after aspartame support the controversy over its effects, and may relate to sweetness perception. PMID:10690159

  11. Human perception, productivity and symptoms related to indoor air quality

    Energy Technology Data Exchange (ETDEWEB)

    Wargocki, P.

    1998-08-01

    Three objectives of the present study are formulated: (1) to investigate whether total sensory pollution load on the air in space can be estimated by adding sensory pollution loads from the individual pollution sources; (2) to develop alternative reference exposures which can be used to calibrate sensory evaluations of the air quality indoors made by trained subjects; and (3) to investigate whether decreasing the pollution loads on the air indoors is an effective measure for improving the perceived air quality, reducing the prevalence of health symptoms and increasing people`s productivity. Limited data exist on the addition of families of sensory pollution, sources, i.e., building materials, people and tobacco smoke (research was mainly performed on building materials), and that no field study on addition has been carried out previously. Consequently, laboratory and field experiments on the addition of families of sensory pollution sources were undertaken. Reducing the sensory pollution load on the air indoors proved to be an effective and energy-efficient measure to improve the perceived quality of air, to lower the prevalence of symptoms and to improve productivity. Suggestions for future experiments are made including, i.a., using other sub-populations of subjects stratified for age, sensitivity and type of work, other pollution sources, as well as the independent measures design and repeated exposures to the same environmental conditions. (EG) 209 refs.

  12. 78 FR 53479 - Certain Laundry and Household Cleaning Products and Related Packaging

    Science.gov (United States)

    2013-08-29

    ... From the Federal Register Online via the Government Publishing Office ] INTERNATIONAL TRADE COMMISSION Certain Laundry and Household Cleaning Products and Related Packaging AGENCY: U.S. International... States after importation of certain laundry and household cleaning products and packaging thereof...

  13. European Group for Blood and Marrow Transplantation Centers with FACT-JACIE Accreditation Have Significantly Better Compliance with Related Donor Care Standards.

    Science.gov (United States)

    Anthias, Chloe; O'Donnell, Paul V; Kiefer, Deidre M; Yared, Jean; Norkin, Maxim; Anderlini, Paolo; Savani, Bipin N; Diaz, Miguel A; Bitan, Menachem; Halter, Joerg P; Logan, Brent R; Switzer, Galen E; Pulsipher, Michael A; Confer, Dennis L; Shaw, Bronwen E

    2016-03-01

    Previous studies have identified healthcare practices that may place undue pressure on related donors (RDs) of hematopoietic cell products and an increase in serious adverse events associated with morbidities in this population. As a result, specific requirements to safeguard RD health have been introduced to Foundation for the Accreditation of Cellular Therapy/The Joint Accreditation Committee ISCT and EBMT (FACT-JACIE) Standards, but the impact of accreditation on RD care has not previously been evaluated. A survey of transplant program directors of European Group for Blood and Marrow Transplantation member centers was conducted by the Donor Health and Safety Working Committee of the Center for International Blood and Marrow Transplant Research to test the hypothesis that RD care in FACT-JACIE accredited centers is more closely aligned with international consensus donor care recommendations than RD care delivered in centers without accreditation. Responses were received from 39% of 304 centers. Our results show that practice in accredited centers was much closer to recommended standards as compared with nonaccredited centers. Specifically, a higher percentage of accredited centers use eligibility criteria to assess RDs (93% versus 78%; P = .02), and a lower percentage have a single physician simultaneously responsible for an RD and their recipient (14% versus 35%; P = .008). In contrast, where regulatory standards do not exist, both accredited and nonaccredited centers fell short of accepted best practice. These results raise concerns that despite improvements in care, current practice can place undue pressure on donors and may increase the risk of donation-associated adverse events. We recommend measures to address these issues through enhancement of regulatory standards as well as national initiatives to standardize RD care. PMID:26597079

  14. Phylogeny, diversity and toxin production related to cyanobacterial symbioses

    OpenAIRE

    Papaefthimiou, Dimitra

    2007-01-01

    Phylogeny and morphology were examined for the cyanobionts from the water fern Azolla and the cyanobacterial genus Nostoc originating from symbioses with different host plants (genera Gunnera, Cycas, Dioon, Encephalarthos, Macrozamia, and Anthoceros), the lichen genus Pannaria, and free-living Nostoc isolates from different habitats. Nostoc isolates of Pannaria formed a closely related group, but, in general, no monophyletic nature was attributed to the genus Nostoc, in contrast to the cyanob...

  15. Public Relations: Ketchum brands new product with innovative PR strategy.

    Science.gov (United States)

    2006-01-01

    Novartis Pharmaceuticals tapped publis relations (PR) giant Ketchum earlier this year to spearhead a marketing campaign that would make its new U.S. Food and Drug Administration-approved prescription-based medication, Enablex, stand out among the crowd in over active bladder (OAB) treatment. OAB is a disorder that affects an estimated 33 million Americans making this treatment highly in demand with consumers and highly competitive with other pharmaceutical companies. PMID:17048759

  16. Morphometric Evaluation of Blood Pressure Regulating Organs in Teddy Goats (Capra hircus in Relation to Age and Sex

    Directory of Open Access Journals (Sweden)

    M. Shah, A. S. Qureshi*1, S. Rehan1 and R. Hussain1

    2010-01-01

    Full Text Available In this study the heart, kidneys and adrenal glands of 36 teddy goats (Capra hircus of both sexes, divided in 3 age groups viz. kids (6-12 months, adults (13-21 months and old (22-24 months were collected after slaughter. Immediately after collection, absolute and relative weights, length, width, thickness, circumference and volume of all organs were recorded. Shape of the heart was cone like and the coronary groove was filled with fat. None of the anatomical parameters of the heart, kidneys and adrenal glands differed between male and female goats, except that absolute weight of the right kidney and volume of right and left kidneys were higher in males than in females (P<0.05. Absolute and relative weights of the heart, volume, length, circumference, right atrial wall thickness and right ventricle wall thickness were higher in old than in kids or adult animals (P<0.05. No difference was seen in various anatomical parameters between the right and the left kidneys. However, values of most of the anatomical parameters were higher in old than in kids or adult goats (P<0.05, except relative weight of the organ and thickness of medulla, which did not differ among animals of three age groups. For adrenals, the absolute weight and length of the left organ were higher than the right (P<0.05. Similarly, absolute weight, length and width were higher in old than in kids (P<0.05. It is conceivable from these findings that goat has a stable cardiovascular system. The development of heart, kidneys and adrenals showed an increase parallel to the advancing age to adjust with the increasing blood pressure due to physiological development process. Sex, however, played a secondary role.

  17. Social support buffering of the relation between low income and elevated blood pressure in at-risk African-American adults.

    Science.gov (United States)

    Coulon, S M; Wilson, D K

    2015-10-01

    Socioeconomic disadvantage has been linked to elevated blood pressure (BP), and the purpose of this study was to assess whether interpersonal social supports buffer these adverse relations in African-American adults. In three communities matched demographically, a subsample of participants (N = 204) of the Positive Action for Today's Health trial provided measures of perceived social support, annual household income, and BP. Multiple regression analyses with cross-product interactions were conducted using follow-up data. The sample had a mean age of 52.8 years (SD = 15.1), and was predominantly female (66 %) with a high body mass index (M = 33.5, SD = 14.7). Results indicated an inverse relation between social support and diastolic BP (B = -.178, p = .005), and also an interaction with income (p = .046), such that higher social support related to lower diastolic BP in the lowest-income individuals (B = -1.05). The same direct (B = -.141, p = .025) and interacting (B = -1.42, p = .040) social support effects were present for systolic BP, however the omnibus model for systolic BP was not significant, F(6, 196) = 1.80, p = .09. The hypothesized buffering effect of social support on the adverse relation of income to BP was partially supported in at-risk African-American adults. Future prevention efforts for reducing the impact of socioeconomic stress on BP may aim to increase perceptions of social support. PMID:26156119

  18. Product Recall versus Business as Usual: A Preliminary Analysis of Decision-Making in Potential Product-related Crises

    OpenAIRE

    Standop, Dirk

    2006-01-01

    Product recalls have become an almost familiar phenomenon in a consumers daily life. The reasons for this include the increasingly advanced and at the same time vulnerable technology of consumer products and the emergent law of strict liability of manufacturers and dealers in the US and Europe. This paper deals with the seller's choice situation. The seller has more or less vague evidence of potential damages which could possibly be related to the use or misuse of his products, altogether ind...

  19. Shifts and Twists in the Relative Productivity of Skilled Labor: Reconciling Accelerated SBTC with the Productivity Slowdown

    OpenAIRE

    Philip Marey; Arnaud Dupuy

    2004-01-01

    Skill-biased technical change is usually interpreted in terms of the efficiency parameters of skilled and unskilled labor. This implies that the relative productivity of skilled workers changes proportionally in all tasks. In contrast, we argue that technical changes also affect the curvature of the distribution of relative productivity. Building on Rosen's (1978) tasks assignment model, this implies that not only the efficiency parameters of skilled and unskilled workers change, but also the...

  20. Biosynthesis Pathway Related Production of Medium Chain Length Polyhydroxyalkanoates

    Institute of Scientific and Technical Information of China (English)

    林会兰; 史文慧; 吴琼; 陈金春; 徐军; 陈国强

    2001-01-01

    Pseudomonas flava HBE06 isolated from oil-contaminated soil was found to produce polyesters consisting of medium chain length polyhydroxyalkanoates (mci PHA). The strain mainly synthesized PHA containing 3-hydroxyoctanoate (C8 or HO), 3-hydroxynonanoate (C9 or HN), 3-hydroxydecanoate (C10 or HD), and 3-hydroxyunidecanoate (C11 or HUD) as monomers when grown on various substrates. It was found that the monomer ratio (especially C10/C8 or C11/C9) was related to the PHA biosynthesis pathway. When PHA was synthesized via the de novo fatty acid pathway, the molar ratio of C10/C8 was greater than 2. If PHA was synthesized from β-oxidation of fatty acids, C10/C8 was less than 1. Pseudomonas stutzeri 1317 is another mci PHA synthesizing bacteria whose C10/C8 ratio is also related to the synthesis pathway. When the two synthesis pathways were used together, the C10/C8 ratio could be adjusted according to the ratio of the mixed substrates.

  1. Retrospective evaluation of adverse transfusion reactions following blood product transfusion from a tertiary care hospital: A preliminary step towards hemovigilance

    OpenAIRE

    Praveen Kumar; Rakesh Thapliyal; Poonam Coshic; Kabita Chatterjee

    2013-01-01

    Background: The goal of hemovigilance is to increase the safety and quality of blood transfusion. Identification of the adverse reactions will help in taking appropriate steps to reduce their incidence and make blood transfusion process as safe as possible. Aims : To determine the frequency and type of transfusion reactions (TRs) occurring in patients, reported to the blood bank at our institute. Materials and Methods : A retrospective review of all TRs reported to the blood bank at the All I...

  2. Blood flow with 133Xe in human nasal mucosa in relation to age, sex and body position

    International Nuclear Information System (INIS)

    The blood flow in human nasal mucosa was determined using the 133Xe wash-out method in 80 subjects of both sexes. The blood flow was estimated to (mean+-SD) 33+-10 ml x min-1 x (100 g) -1. A correlation was found between decreasing blood flow and increasing age. There was no difference between the sexes. The blood flow was significantly decreased in the sitting position compared to the supine position. In repeated determinations, the first recording did not disturb the second one 15 to 60 min later. The coefficient of variation was estimated to 22%. The investigation gives reference values for further studies of the blood flow in the human nasal mucosa. (Author)

  3. THE EFFECT OF A NEW SALICYLATE SYNTHESIS PRODUCT ON BLOOD GSH VALUES IN RATS

    Directory of Open Access Journals (Sweden)

    CORINA GRĂVILĂ

    2013-12-01

    Full Text Available GSH (γ-glutamylcysteinylglycine is a sulfhydril (-SH antioxidant, antitoxin and enzyme cofactor which is an important component of the cellular detoxification of reactive oxygen species (ROS. Being water soluble it is found mainly in the cytosol and other aqueous phases of the living system and thus constitute one of the most important intracellular antioxidants (10,7,9. GSH plays a role in removing various toxic chemicals and drugs from the body. As a result glutathione levels in the body are reduced by exposure to heavy metals and the chemicals used in chemotherapy (6. Sulfanilamide was the first sulfonamide discovered in this class of antimicrobial agents and its structure is considered to contain the minimum pharmacophore. They prevent or limit bacterial multiplication. Salicylic acid (2-hydroxybenzoic acid, is the basic substance of the salicylates which are non-steroidal anti-inflammatory drugs (NSAIDs. Salicylic acid and methyl salicylate (ester (methyl 2-hydroxybenzoate are the main therapeutically used substances of this group. This study was carried out to investigate the effect of a new synthesis product in comparison with the effect of sulfanilamide on GSH values in intraperitonally injected Wistar rats.

  4. River ice implications related to water power production in Norway

    Energy Technology Data Exchange (ETDEWEB)

    Asvall, R.P. [Norwegian Water Resources and Energy Directorate, Oslo (Norway). Hydrology Dept.

    2009-07-01

    Nearly 99 per cent of the electricity produced in Norway is based on water power. While the period of large power development is over, the current focus lies in developing small hydroelectric power plants. A new market based energy law was implemented in Norway in 1991 to achieve more efficient use of electricity production by means of market forces. Since water regulation influences ice conditions in lakes and rivers, this paper focused on the implications of changes in ice conditions. In Norway, the expected changes in ice conditions are taken into account when issuing permits for water regulations and schemes for water discharge because some waterways are used as winter roads. Follow-up includes both close and long term observations and measurements. The impact of variable price on power was also discussed, with particular reference to ice conditions in cases where water discharge occurs on rivers. This paper summarized selected ice problems and how they have been handled. The paper also included a summary of anticipated climatic changes relevant to ice conditions.

  5. Vitamin C in plasma is inversely related to blood pressure and change in blood pressure during the previous year in young Black and White women

    Directory of Open Access Journals (Sweden)

    Hudes Mark

    2008-12-01

    Full Text Available Abstract Background The prevalence of hypertension and its contribution to cardiovascular disease risk makes it imperative to identify factors that may help prevent this disorder. Extensive biological and biochemical data suggest that plasma ascorbic acid may be such a factor. In this study we examined the association between plasma ascorbic acid concentration and blood pressure (BP in young-adult women. Methods Participants were 242 Black and White women aged 18–21 yr from the Richmond, CA, cohort of the National Heart, Lung and Blood Institute Growth and Health Study. We examined the associations of plasma ascorbic acid with BP at follow-up year 10, and with change in BP during the previous year. Results In cross-sectional analysis, plasma ascorbic acid at year 10 was inversely associated with systolic BP and diastolic BP after adjusting for race, body mass index, education, and dietary intake of fat and sodium. Persons in the highest one-fourth of the plasma ascorbic acid distribution had 4.66 mmHg lower systolic BP (95% CI 1.10 to 8.22 mmHg, p = 0.005 and 6.04 mmHg lower diastolic BP (95% CI 2.70 to 9.38 mmHg, p = 0.0002 than those in the lowest one-fourth of the distribution. In analysis of the change in BP, plasma ascorbic acid was also inversely associated with change in systolic BP and diastolic BP during the previous year. While diastolic blood pressure among persons in the lowest quartile of plasma ascorbic acid increased by 5.97 mmHg (95% CI 3.82 to 8.13 mmHg from year 9 to year 10, those in the highest quartile of plasma vitamin C increased by only 0.23 mmHg (95% CI -1.90 to +2.36 mmHg (test for linear trend: p Conclusion Plasma ascorbic acid was found to be inversely associated with BP and change in BP during the prior year. The findings suggest the possibility that vitamin C may influence BP in healthy young adults. Since lower BP in young adulthood may lead to lower BP and decreased incidence of age-associated vascular events in

  6. Bacillus Calmette-Guérin enhances production and secretion of type IV collagenases in peripheral blood mononuclear cells.

    Science.gov (United States)

    Kageyama, Y; Kawakami, S; Fujii, Y; Kihara, K; Oshima, H

    1997-03-01

    Intravesical administration of bacillus Calmette-Guérin (BCG) is an effective and widely accepted treatment for superficial bladder cancer. Rapid progression of the disease after BCG therapy, however, has been reported in some cases refractory to the treatment. We examined whether BCG treatment and coexistence of peripheral blood mononuclear cells (PBMCs) alter the invasive potential of bladder cancer cells. Production and secretion of two type IV collagenases, matrix metalloproteinase (MMP) 2 and MMP 9, by PBMCs from five healthy donors or bladder cancer cells (T24, JTC 30, and JTC 32) were evaluated by gelatin zymography, western blot analysis, and northern blot analysis. Invasion of bladder cancer cells was also examined using reconstituted basement membrane (Matrigel). BCG (5, 50, and 500 micrograms/ml) had no effect on secretion of MMP 2 and MMP 9 by bladder cancer cells, but increased the production and secretion of MMP 9 by PBMCs in a dose-dependent manner. The coexistence of PBMCs increased invasion of T24 cells and BCG further enhanced the invasion. Thus, BCG promotes invasion of bladder cancer cells under certain conditions. An increase in the secretion of MMP 9 by PBMCs may account in part for the effect. PMID:9140113

  7. Senescence-Related Changes in Gene Expression of Peripheral Blood Mononuclear Cells from Octo/Nonagenarians Compared to Their Offspring

    Directory of Open Access Journals (Sweden)

    Amirah Abdul Rahman

    2013-01-01

    Full Text Available Mechanisms determining both functional rate of decline and the time of onset in aging remain elusive. Studies of the aging process especially those involving the comparison of long-lived individuals and young controls are fairly limited. Therefore, this research aims to determine the differential gene expression profile in related individuals from villages in Pahang, Malaysia. Genome-wide microarray analysis of 18 samples of peripheral blood mononuclear cells (PBMCs from two groups: octo/nonagenarians (80–99 years old and their offspring (50.2 ± 4.0 years old revealed that 477 transcripts were age-induced and 335 transcripts were age-repressed with fold changes ≥1.2 in octo/nonagenarians compared to offspring. Interestingly, changes in gene expression were associated with increased capacity for apoptosis (BAK1, cell cycle regulation (CDKN1B, metabolic process (LRPAP1, insulin action (IGF2R, and increased immune and inflammatory response (IL27RA, whereas response to stress (HSPA8, damage stimulus (XRCC6, and chromatin remodelling (TINF2 pathways were downregulated in octo/nonagenarians. These results suggested that systemic telomere maintenance, metabolism, cell signalling, and redox regulation may be important for individuals to maintain their healthy state with advancing age and that these processes play an important role in the determination of the healthy life-span.

  8. Effects of different fibre sources and fat addition on cholesterol and cholesterol-related lipids in blood serum, bile and body tissues of growing pigs.

    Science.gov (United States)

    Kreuzer, M; Hanneken, H; Wittmann, M; Gerdemann, M M; Machmuller, A

    2002-04-01

    Knowledge is limited on the efficacy of hindgut-fermentable dietary fibre to reduce blood, bile and body tissue cholesterol levels. In three experiments with growing pigs the effects of different kinds and levels of bacterially fermentable fibre (BFS) on cholesterol metabolism were examined. Various diets calculated to have similar contents of metabolizable energy were supplied for complete fattening periods. In the first experiment, a stepwise increase from 12 to 20% BFS was performed by supplementing diets with fermentable fibre from sugar beet pulp (modelling hemicelluloses and pectin). Beet pulp, rye bran (modelling cellulose) and citrus pulp (pectin) were offered either independently or in a mixture in the second experiment. These diets were opposed to rations characterized in carbohydrate type by starch either mostly non-resistant (cassava) or partly resistant (maize) to small intestinal digestion. The third experiment was planned to explore the interactions of BFS from citrus pulp with fat either through additional coconut oil/palm kernel oil blend or full-fat soybeans. In all experiments the increase of the BFS content was associated with a constant (cellulose) or decreasing (hemicelluloses, pectin) dietary proportion of non-digestible fibre. In experiment 1 an inverse dose-response relationship between BFS content and cholesterol in blood serum and adipose tissue as well as bile acid concentration in bile was noted while muscle cholesterol did not respond. In experiment 2 the ingredients characterized by cellulose and hemicelluloses/pectin reduced cholesterol-related traits relative to the low-BFS-high-starch controls whereas, except in adipose tissue cholesterol content, the pectinous ingredient had the opposite effect. However, the changes in serum cholesterol mainly affected HDL and not LDL cholesterol. Adipose tissue cholesterol also was slightly lower with partly resistant starch compared to non-resistant starch in the diet. Experiment 3 showed that

  9. Uncertainty of Blood Alcohol Concentration (BAC Results as Related to Instrumental Conditions: Optimization and Robustness of BAC Analysis Headspace Parameters

    Directory of Open Access Journals (Sweden)

    Haleigh A. Boswell

    2015-12-01

    Full Text Available Analysis of blood alcohol concentration is a routine analysis performed in many forensic laboratories. This analysis commonly utilizes static headspace sampling, followed by gas chromatography combined with flame ionization detection (GC-FID. Studies have shown several “optimal” methods for instrumental operating conditions, which are intended to yield accurate and precise data. Given that different instruments, sampling methods, application specific columns and parameters are often utilized, it is much less common to find information on the robustness of these reported conditions. A major problem can arise when these “optimal” conditions may not also be robust, thus producing data with higher than desired uncertainty or potentially inaccurate results. The goal of this research was to incorporate the principles of quality by design (QBD in the adjustment and determination of BAC (blood alcohol concentration instrumental headspace parameters, thereby ensuring that minor instrumental variations, which occur as a matter of normal work, do not appreciably affect the final results of this analysis. This study discusses both the QBD principles as well as the results of the experiments, which allow for determination of more favorable instrumental headspace conditions. Additionally, method detection limits will also be reported in order to determine a reporting threshold and the degree of uncertainty at the common threshold value of 0.08 g/dL. Furthermore, the comparison of two internal standards, n-propanol and t-butanol, will be investigated. The study showed that an altered parameter of 85 °C headspace oven temperature and 15 psi headspace vial pressurization produces the lowest percent relative standard deviation of 1.3% when t-butanol is implemented as an internal standard, at least for one very common platform. The study also showed that an altered parameter of 100 °C headspace oven temperature and 15-psi headspace vial pressurization

  10. Efficient removal of platelets from peripheral blood progenitor cell products using a novel micro-chip based acoustophoretic platform.

    Directory of Open Access Journals (Sweden)

    Josefina Dykes

    Full Text Available BACKGROUND: Excessive collection of platelets is an unwanted side effect in current centrifugation-based peripheral blood progenitor cell (PBPC apheresis. We investigated a novel microchip-based acoustophoresis technique, utilizing ultrasonic standing wave forces for the removal of platelets from PBPC products. By applying an acoustic standing wave field onto a continuously flowing cell suspension in a micro channel, cells can be separated from the surrounding media depending on their physical properties. STUDY DESIGN AND METHODS: PBPC samples were obtained from patients (n = 15 and healthy donors (n = 6 and sorted on an acoustophoresis-chip. The acoustic force was set to separate leukocytes from platelets into a target fraction and a waste fraction, respectively. The PBPC samples, the target and the waste fractions were analysed for cell recovery, purity and functionality. RESULTS: The median separation efficiency of leukocytes to the target fraction was 98% whereas platelets were effectively depleted by 89%. PBPC samples and corresponding target fractions were similar in the percentage of CD34+ hematopoetic progenitor/stem cells as well as leukocyte/lymphocyte subset distributions. Median viability was 98%, 98% and 97% in the PBPC samples, the target and the waste fractions, respectively. Results from hematopoietic progenitor cell assays indicated a preserved colony-forming ability post-sorting. Evaluation of platelet activation by P-selectin (CD62P expression revealed a significant increase of CD62P+ platelets in the target (19% and waste fractions (20%, respectively, compared to the PBPC input samples (9%. However, activation was lower when compared to stored blood bank platelet concentrates (48%. CONCLUSION: Acoustophoresis can be utilized to efficiently deplete PBPC samples of platelets, whilst preserving the target stem/progenitor cell and leukocyte cell populations, cell viability and progenitor cell colony-forming ability

  11. Cerebral extraction of N-13 ammonia: its dependence on cerebral blood flow and capillary permeability, surface area product

    International Nuclear Information System (INIS)

    13N-labeled ammonia was used to investigate: (1) the cerebral extraction and clearance of ammonia; (2) the mechanicsm by which capillaries accommodate changes in cerebral blood flow (CBF); and (3) its use for the measure of CBF. This was investigated by measuring the single pass extraction of 13NH3 in rhesus monkeys during P/sub a/CO2 induced changes in CBF, and with dog studies using in vitro tissue counting techniques to examine 13NH3 extraction in gray and white matter, mixed tissue, and cerebellum during variations in CBF produced by combinations of embolization, local brain compression, and changes in P/sub a/CO2. The single pass extraction fraction of 13NH3 varied from about 70 to 20% over a CBF range of 12 to 140cc/min/100gms. Capillary permeability-surface area product (PS) estimates from this data and the dog experiments show PS increasing with CBF. The magnitude and rate of increase in PS with CBF was highest in gray matter > mixed tissue > white matter. Tissue extraction of 13NH3 vs CBF relationship was best described by a unidirectional transport model in which CBF increases by both recruitment of capillaries and by increases of blood velocity in open capillaries. Glutamine synthetase, which incorporates 13NH3 into glutamine, appears to be anatomically located in astrocytes in general and specifically in the astrocytic pericapillary end-feet that are in direct contact with gray and white matter capillaries. The net 13NH3 extraction subsequent to an i.v. injection increases nonlinearly with CBF. Doubling or halving basal CBF produced from 40 to 50% changes in the 13N tissue concentrations with further increases in CBF associated with progressively smaller changes in 13N concentrations. 13NH3 appears to be a good tracer for the detection of cerebral ischemia with positron tomography but exhibits a poor response at high values of CBF

  12. Multiparameter fluorescence mapping of nonprotein sulfhydryl status in relation to blood vessels and hypoxia in cervical carcinoma xenografts

    International Nuclear Information System (INIS)

    Purpose: Aberrant architecture of the tumor vasculature and temporal fluctuations in blood flow can result in tumor hypoxia. The aim of this study was to classify tumor hypoxia based on distance to blood vessels, and to characterize its biologic significance by determining levels of nonprotein sulfhydryls (NPSH) in hypoxic regions located proximally and distally to tumor blood vessels. Methods and Materials: A dual fluorescence method was developed for the spatial colocalization of the vasculature and hypoxia in frozen sections from SiHa cervical carcinoma xenografts. A parallel section was stained with the sulfhydryl stain mercury orange. Composite fluorescence images were generated by imaging and tiling individual fields of view into 2D image arrays. Image arithmetic techniques were combined with feature-based image segmentation to characterize expression of NPSH as a function of the hypoxic tumor microenvironment. Results: NPSH levels were higher in hypoxic areas of the SiHa xenografts (15.1±0.5 vs. 13.5±0.5 integrated optical density [IOD], p<0.03). When tumor hypoxia was classified by distance to the nearest visible blood vessel, significantly higher NPSH levels were found in hypoxic regions close to blood vessels than in regions at a distance from blood vessels. Conclusion: The results of this study indicate differential expression of NPSH levels in regions of hypoxia that are proximal or distal to blood vessels in SiHa tumors

  13. What drives consumer involvement? The relative impact of product category and product attribute

    DEFF Research Database (Denmark)

    de Barcellos, Marcia Dutra; Kügler, Jens Oliver; Scholderer, Joachim

    2009-01-01

    , foods, household appliances, alcoholic beverages) and on two involvement dimensions (interest and importance). The results indicate that in three of the four super-categories the predominant source of variation in product involvement were the attributes of the products, not the product category they...... belong to. Only for foods, the traditional conceptualisation of involvement held. The findings suggest that parts of previous research on involvement may have to be re-interpreted and that future research should adopt more complex conceptualisations of involvement. From a managerial point of view, this...

  14. Phosphoenolpyruvate Carboxykinase, a Key Enzyme That Controls Blood Glucose, Is a Target of Retinoic Acid Receptor-Related Orphan Receptor α.

    Directory of Open Access Journals (Sweden)

    Hiroshi Matsuoka

    Full Text Available Phosphoenolpyruvate carboxykinase (PEPCK catalyzes a committed and rate-limiting step in hepatic gluconeogenesis, and its activity is tightly regulated to maintain blood glucose levels within normal limits. PEPCK activity is primarily regulated through hormonal control of gene transcription. Transcription is additionally regulated via a cAMP response unit, which includes a cAMP response element and four binding sites for CCAAT/enhancer-binding protein (C/EBP. Notably, the cAMP response unit also contains a putative response element for retinoic acid receptor-related orphan receptor α (RORα. In this paper, we characterize the effect of the RORα response element on cAMP-induced transcription. Electrophoresis mobility shift assay indicates that RORα binds this response element in a sequence-specific manner. Furthermore, luciferase reporter assays indicate that RORα interacts with C/EBP at the PEPCK promoter to synergistically enhance transcription. We also found that cAMP-induced transcription depends in part on RORα and its response element. In addition, we show that suppression of RORα by siRNA significantly decreased PEPCK transcription. Finally, we found that a RORα antagonist inhibits hepatic gluconeogenesis in an in vitro glucose production assay. Taken together, the data strongly suggest that PEPCK is a direct RORα target. These results define possible new roles for RORα in hepatic gluconeogenesis.

  15. Relation of soluble RANKL and osteoprotegerin levels in blood and gingival crevicular fluid to the degree of root resorption after orthodontic tooth movement

    International Nuclear Information System (INIS)

    The aim of the present study was the determination of the levels of osteoprotegerin and soluble receptor activator of nuclear factor-(KB) ligand (RANKL) in blood serum and in gingival crevicular fluid relative to the degree of orthodontic root resorption in a rat model. Blood samples and gingival crevicular fluid were collected from fourteen 6-month-old male Wistar rats weighing 350-500 g. A 25-g closed orthodontic coil spring was inserted between each upper right first molar and the upper incisors. After 21 days of loading, both upper first molars (treated and control) were extracted and studied under microcomputed tomography scanning. Statistical analysis demonstrated a positive linear correlation between the initial concentration of RANKL in blood serum and the degree of root resorption. The ratio of the initial concentrations of osteoprotegerin to RANKL in blood serum proved to be an independent prognostic factor of the degree of root resorption. The initial concentration of RANKL in gingival crevicular fluid showed a negative correlation to the initial concentration of RANKL in blood serum and for a finite range of initial concentrations of osteoprotegerin in gingival crevicular fluid, the dental root seemed protected against extreme external root resorption. Finally, the concentration of osteoprotegerin in blood serum decreased significantly in cases of severe root resorption. (author)

  16. Evaluating the Stability of RNA-Seq Transcriptome Profiles and Drug-Induced Immune-Related Expression Changes in Whole Blood.

    Science.gov (United States)

    Bowyer, John F; Tranter, Karen M; Hanig, Joseph P; Crabtree, Nathaniel M; Schleimer, Robert P; George, Nysia I

    2015-01-01

    Methods were developed to evaluate the stability of rat whole blood expression obtained from RNA sequencing (RNA-seq) and assess changes in whole blood transcriptome profiles in experiments replicated over time. Expression was measured in globin-depleted RNA extracted from the whole blood of Sprague-Dawley rats, given either saline (control) or neurotoxic doses of amphetamine (AMPH). The experiment was repeated four times (paired control and AMPH groups) over a 2-year span. The transcriptome of the control and AMPH-treated groups was evaluated on: 1) transcript levels for ribosomal protein subunits; 2) relative expression of immune-related genes; 3) stability of the control transcriptome over 2 years; and 4) stability of the effects of AMPH on immune-related genes over 2 years. All, except one, of the 70 genes that encode the 80s ribosome had levels that ranked in the top 5% of all mean expression levels. Deviations in sequencing performance led to significant changes in the ribosomal transcripts. The overall expression profile of immune-related genes and genes specific to monocytes, T-cells or B-cells were well represented and consistent within treatment groups. There were no differences between the levels of ribosomal transcripts in time-matched control and AMPH groups but significant differences in the expression of immune-related genes between control and AMPH groups. AMPH significantly increased expression of some genes related to monocytes but down-regulated those specific to T-cells. These changes were partially due to changes in the two types of leukocytes present in blood, which indicate an activation of the innate immune system by AMPH. Thus, the stability of RNA-seq whole blood transcriptome can be verified by assessing ribosomal protein subunits and immune-related gene expression. Such stability enables the pooling of samples from replicate experiments to carry out differential expression analysis with acceptable power. PMID:26177368

  17. Evaluating the Stability of RNA-Seq Transcriptome Profiles and Drug-Induced Immune-Related Expression Changes in Whole Blood.

    Directory of Open Access Journals (Sweden)

    John F Bowyer

    Full Text Available Methods were developed to evaluate the stability of rat whole blood expression obtained from RNA sequencing (RNA-seq and assess changes in whole blood transcriptome profiles in experiments replicated over time. Expression was measured in globin-depleted RNA extracted from the whole blood of Sprague-Dawley rats, given either saline (control or neurotoxic doses of amphetamine (AMPH. The experiment was repeated four times (paired control and AMPH groups over a 2-year span. The transcriptome of the control and AMPH-treated groups was evaluated on: 1 transcript levels for ribosomal protein subunits; 2 relative expression of immune-related genes; 3 stability of the control transcriptome over 2 years; and 4 stability of the effects of AMPH on immune-related genes over 2 years. All, except one, of the 70 genes that encode the 80s ribosome had levels that ranked in the top 5% of all mean expression levels. Deviations in sequencing performance led to significant changes in the ribosomal transcripts. The overall expression profile of immune-related genes and genes specific to monocytes, T-cells or B-cells were well represented and consistent within treatment groups. There were no differences between the levels of ribosomal transcripts in time-matched control and AMPH groups but significant differences in the expression of immune-related genes between control and AMPH groups. AMPH significantly increased expression of some genes related to monocytes but down-regulated those specific to T-cells. These changes were partially due to changes in the two types of leukocytes present in blood, which indicate an activation of the innate immune system by AMPH. Thus, the stability of RNA-seq whole blood transcriptome can be verified by assessing ribosomal protein subunits and immune-related gene expression. Such stability enables the pooling of samples from replicate experiments to carry out differential expression analysis with acceptable power.

  18. Commonalities and differences between production-related FDI (PFDI) and technology-related FDI (TFDI) in developed and emerging economies

    OpenAIRE

    Alvandi , Keyvan; Chaminade , Cristina; Lv, Ping

    2013-01-01

    This paper investigates commonalities and differences in firm level determinants of internationalization of production (production related investments or PFDI) and innovation (technology driven investments or TFDI) by Multinational Enterprises (MNEs). Our database is based on a cross country survey which includes firms within Automotive, Agro-processing and ICT sectors from both developing and advanced economies. Our results show that despite some differences, most of the determinants affect ...

  19. The relative labour productivity contribution of different age-skill categories for a developing economy

    Directory of Open Access Journals (Sweden)

    Gerhardus van Zyl

    2013-01-01

    Full Text Available Orientation: The article dealt with the estimation, computation and interpretation of the relative productivity contributions of different age-skill categories.Research purpose: The aim of the article was to estimate and compute, (1 relative productivity contributions and (2 relative productivity contribution–employee remuneration cost levels for different age-skill categories.Motivation for the study: The research was deemed necessary given the current debate on relative productivity levels and possible changes to the retirement age in the South African labour market. No real research in this regard has been published regarding the South African labour market situation.Research design, approach and method: A less restrictive production function was used, allowing for the simultaneous estimation and final computation of relative labour contribution levels of different age-skill categories.Main findings: The lower-skilled segment produced significantly smaller productivity contributions and the relative productivity contribution–employee remuneration cost ratios of the 55 years and older age group were superior in the higher-skilled segment but, at the same time, the lowest in the lower-skilled segment.Practical/managerial implications: It is recommended that human resource practitioners (given the perceived rigidity of labour legislation implement and maintain structures that promote higher productivity levels for all age-skill categories in the workplace.Contribution/value-add: An estimation procedure, which can be applied to the measurement of the relative productivity contribution of different age-skill categories, has been established.

  20. 76 FR 33409 - Guidance on Deposit-Related Consumer Credit Products

    Science.gov (United States)

    2011-06-08

    ... these products to take advantage of their customer relationship. Through its supervisory process, the.... Management oversight--Bank management should exercise appropriate oversight of new products and services... services related to the product. Bank management should be vigilant in assuring adherence to...