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Sample records for blood protein von

  1. Identifizierung und Charakterisierung eines Sdr-Proteins von Staphylococcus saprophyticus

    OpenAIRE

    Sakinç, Türkân

    2001-01-01

    Gegenstand der vorliegenden Arbeit war es, das Oberflächenprotein Ssp zu klonieren und zu charakterisieren. Stattdessen wurde ein neues Zellwand-assoziertes Protein, das SdrI, ein MSCRAMM, kloniert. Dies sollte zur Klärung der Uropathogenität von Staphylococcus saprophyticus weiterhelfen. Die Voraussetzungen zur Untersuchung der Funktion des SdrI Protein von Staphylococcus saprophyticus wurde durch die Konstruktion einer isogenen Mutante geschaffen. Die Bedeutung der SdrI-Protein ...

  2. No evidence for a direct effect of von Willebrand factor's ABH blood group antigens on von Willebrand factor clearance

    NARCIS (Netherlands)

    Groeneveld, D J; van Bekkum, T; Cheung, K L; Dirven, R J; Castaman, G; Reitsma, P H; van Vlijmen, B; Eikenboom, J

    2015-01-01

    BACKGROUND: One of the major determinants of von Willebrand factor (VWF) plasma levels is ABO blood group status, and individuals with blood group O have ~ 25% lower plasma levels. The exact mechanism behind this relationship remains unknown, although effects on clearance have been postulated. OBJEC

  3. Biological Variations of Lupus Anticoagulant, Antithrombin, Protein C, Protein S, and von Willebrand Factor Assays.

    Science.gov (United States)

    Shou, Weiling; Chen, Qian; Wu, Wei; Cui, Wei

    2016-02-01

    The results of lupus anticoagulant (LA), antithrombin (AT), protein C (PC), and protein S (PS) testing, and the values of von Willebrand factor antigen (VWF:Ag) are important in diagnosis and therapeutic monitoring of thrombosis and hemostasis diseases. Till now, no published study has focused on the biological variations in LA testing, and only a few studies have examined the biological variations of AT, PC, PS, and VWF:Ag. With the latest fully automated instruments and improved reagents, the analytical, within-subject, and between-subject biological variations were estimated for these five coagulant parameters in a cohort of 25 apparently healthy subjects. Blood specimens were collected at 8:00 am, 12:00 pm, and 4:00 pm on days 1, 3, and 5. The analytical biological variation (CV(A)) values of all the parameters were less than 3%. The within-subject biological variation (CV(W)) and between-subject biological variation (CV(G)) values of the LA normalized ratio were 4.64 and 6.83%, respectively. No significant differences were observed in the intraday and interday biological variations of LA tests, or in AT, PC, PS, and VWF:Ag values. Additionally, the utility of the conventional population-based reference intervals of the five coagulation parameters was evaluated by the index of individuality, and data on CV(W) and CV(A) were used to calculate the reference change value to identify the significance of changes in serial results from the same individual. PMID:26516946

  4. Untersuchungen zur Expression und Regulation von murinem Bactericidal/ Permeability Increasing Protein (BPI)

    OpenAIRE

    Eckert, Melanie

    2007-01-01

    LPS-bindende Proteine repräsentieren eine wichtige Säule bei der Bekämpfung von gramnegativen Bakterien durch das angeborene Immunsystem. Zum einen liefern sie einen wichtigen Beitrag bei der hochsensitiven Erkennung gramnegativer Bakterien, zum anderen können sie durch die Neutralisation von LPS die Ausbildung eines septischen Schocks durch eine überschießende Immunantwort verhindern. Humanes Bactericidal/ Permeability Increasing Protein (BPI) ist als LPS-bindendes Protein beschrieben. Es ze...

  5. Immunolocalization of von Willebrand protein in Weibel-Palade bodies of human endothelial cells

    OpenAIRE

    1982-01-01

    Immunofluorescence staining of cultured human umbilical vein endothelial cells has shown the presence of von Willebrand protein in the perinuclear region, in small rodlike structures through the cytoplasm, and on filaments of the extracellular matrix. Nonendothelial cells showed no staining with anti-von Willebrand protein antiserum. At the light microscope level, immunoperoxidase treatment of endothelial cells revealed the same pattern and antibody specificity as the fluorescence staining. T...

  6. CYPRINIDS TOTAL BLOOD PROTEINS DETERMINATION

    Directory of Open Access Journals (Sweden)

    TANŢI PATRICHE

    2013-07-01

    Full Text Available In aquaculture to get a high production is conditioned by awareness and keeping of an unaltered health condition of the biological material. To be aware of the health condition of the biological material in a fish farm allows us to establish the preventive measures required to prevent spreading of a disease and the treatment to be applied in case that a mass disease occurs. The level of the total protein in serum is, first of all, a synthetically indicator of the nutritional condition of the organism, presenting, at the same time, ample qualitative and quantitative variations depending on species, age, sex, stage of sexual maturity, water temperature and especially in correlation with the health condition of fish. Modification in value of the total protein point out some metabolic perturbations in fish body.

  7. Blood protein adsorption onto chitosan

    OpenAIRE

    Benesch, Johan; Tengvall, P.

    2002-01-01

    Chitosan was recently indicated to enhance osteogenesis, improve wound healing but to activate the coagulation and the complement systems. In the present study approximately 10nm thick chitosan film were prepared on aminopropyltriethoxysilane (APTES) coated silicon. The surfaces were incubated in serum or plasma and subsequently in antibodies towards key complement and contact activation of coagulation proteins. The deposited amounts were compared with those on hydrophilic and hydrop...

  8. Charakterisierung von Nanopartikel-Protein-Agglomeraten in biologisch relevanten Medien

    OpenAIRE

    Lang, Thomas

    2014-01-01

    In der vorliegenden Forschungsarbeit wurden drei unterschiedliche Nanomaterialien untersucht, welche unter physiologischen Bedingungen ein unterschiedliches Verhalten aufwiesen. Bei diesen Materialien handelte es sich um stabilisatorfreie Legierungs-Nanopartikel aus der Laser-Ablation, amorphe Silica- sowie um Poly(organosiloxan) Nanopartikel. Im Hinblick auf in vitro Zellstudien wurde eine Größencharakterisierung in Zellmedium in Gegenwart von Proteinen aus fötalem Rinderserum durchgeführt. ...

  9. Die Synthese von Glycopeptiden und Glycopeptid-Protein-Konjugaten mit einer Partialstruktur des tumorassoziierten Mucins MUC1 zur Entwicklung von Tumorvakzinen

    OpenAIRE

    Wittrock, Sven

    2006-01-01

    „Synthese von Glycopeptiden und Glycopeptid-Protein-Konjugaten mit einer Partialstruktur des tumorassoziierten Mucins MUC1 zur Entwicklung von Tumorvakzinen“ Das Glycoprotein MUC1 ist in Tumorepithelzellen sonderlich stark überexprimiert und wegen der vorzeitig einsetzenden Sialylierung sind die Saccharid-Epitope der O-Glycanketten stark verkürzt (sog. tumorassoziierte Antigene). Dadurch werden auch bisher verborgene Peptidepitope des Glycoprotein-Rückgrates auf der Zelloberfläche der Epi...

  10. Functional Human Blood Protein Obtained from Rice

    Institute of Scientific and Technical Information of China (English)

    2011-01-01

    Under a research project funded by NSFC,Dr.Yang He of College of Life Sciences,Wuhan University obtained functional human blood protein from rice,and published their research findings in an article "Large-scale production of functional human serum albumin from transgenic rice seeds" on PNAS in November 2011.

  11. Analysen zur Funktion des E1B-55K-Proteins von Adenovirus Typ 5 im lytischen Replikationszyklus

    OpenAIRE

    Kindsmüller, Kathrin

    2007-01-01

    Das E1B-55K-Protein von Adenovirus Typ 5 ist ein multifunktionelles Phosphoprotein, das eine zentrale Rolle im produktiven Replikationszyklus von Ad5 einnimmt. Die lytischen Aktivitäten des E1B-Proteins werden zumindest teilweise im Komplex mit dem viralen Protein E4orf6 vermittelt. Soweit bekannt fördert der E1B-55K/E4orf6-Komplex (E1B/E4-Komplex) den nukleozytoplasmatischen Transport viraler mRNAs und fördert somit die Synthese viraler Kapsidproteine bzw. die Produktion von Nachkommenviren....

  12. Biosynthesis of von Willebrand protein by human endothelial cells: processing steps and their intracellular localization

    OpenAIRE

    1984-01-01

    Biosynthesis of von Willebrand protein by human umbilical vein endothelial cells involved distinct processing steps marked by the presence of several intermediate molecular species. Examination of endoglycosidase H sensitivity of these intracellular intermediates indicated that the processing steps occurred in at least two separate cellular compartments. In the pre-Golgi apparatus (most probably the endoplasmic reticulum), the high mannose carbohydrates were added onto the precursor monomer c...

  13. Pif97, a von Willebrand and Peritrophin Biomineralization Protein, Organizes Mineral Nanoparticles and Creates Intracrystalline Nanochambers.

    Science.gov (United States)

    Chang, Eric P; Evans, John Spencer

    2015-09-01

    The formation of the mollusk nacre layer involves the assembly and organization of mineral nanoparticles into fracture-toughened mesoscale-sized aragonite tablets that possess intracrystalline nanoporosities. At least one nacre protein family, known as the framework proteome, is strategically located as part of a macromolecular coating around each nacre tablet and is believed to participate in tablet formation. Here, we report new studies of a recombinant form (rPif97) of a unique Japanese pearl oyster (Pinctada fucata) nacre framework biomineralization protein, Pif97. This unique protein possesses both a von Willlebrand factor type A domain (vWA, F23-Y161) and a Peritrophin A chitin-binding domain (PAC, E234-D298). rPif97 self-associates or aggregates to form amorphous protein phases that organize both amorphous and single-crystal calcium carbonate nanoparticles in vitro. Further, in the presence of nucleating calcite crystals, rPif97 protein phases deposit onto these crystals and become occluded over time, forming nanochambers within the crystal interior. The formation of these mineral-modifying amorphous protein phases is linked to the presence of intrinsic disorder and amyloid-like cross-β-strand aggregation-prone regions, and three-dimensional modeling indicates that both the vWA and PAC domains are accessible for intermolecular interactions. Thus, the vWA- and PAC-containing Pif97 protein exhibits key functionalities that would allow its participation in mollusk nacre layer tablet assembly and porosity formation. PMID:26258941

  14. Blood-feeding and immunogenic aedes aegypti saliva proteins

    OpenAIRE

    Surasombatpattana, Pornapat; Wasinpiyamongkol, L.; Patramool, Sirilaksana; Luplertlop, N.; Doucouré, Souleymane; Mouchet, François; Seveno, M.; Remoué, Franck; Demettre, E.; Brizard, Jean-Paul; Jouin, P.; Biron, D.G; F. Thomas; Missé, Dorothée

    2010-01-01

    Mosquito-transmitted pathogens pass through the insect's midgut (MG) and salivary gland (SG). What occurs in these organs in response to a blood meal is poorly understood, but identifying the physiological differences between sugar-fed and blood-fed (BF) mosquitoes could shed light on factors important in pathogens transmission. We compared differential protein expression in the MGs and SGs of female Aedes aegypti mosquitoes after a sugar- or blood-based diet. No difference was observed in th...

  15. Interaction of blood plasma with protein resistant surfaces

    Czech Academy of Sciences Publication Activity Database

    Brynda, Eduard; Riedel, Tomáš; Rodriguez-Emmenegger, Cesar; Reicheltová, Z.; Májek, P.

    Strasbourg: European Materials Research Society, 2013. RP.1-13. [E- MRS 2013 Spring Meeting. 27.05.2013-31.05.2013, Strasbourg] Institutional support: RVO:61389013 Keywords : blood plasma * protein adsorption Subject RIV: CD - Macromolecular Chemistry

  16. Molecular cloning and characterization of the von Hippel-Lindau-like protein.

    Science.gov (United States)

    Qi, Heng; Gervais, Michelle L; Li, Wei; DeCaprio, James A; Challis, John R G; Ohh, Michael

    2004-01-01

    von Hippel-Lindau (VHL) tumor suppressor protein-inactivated in VHL disease and sporadic kidney cancer-is a component of an E3 ubiquitin ligase complex that selectively ubiquitinates the alpha subunit of the hypoxia-inducible factor (HIF) transcription factor for subsequent destruction by the 26S proteasome. Here, we report the identification and characterization of the first VHL homologue, VHL-like protein (VLP), located on chromosome 1q21.2. A 676-bp partial cDNA encoding a 139-amino acid protein that is 78% similar to VHL was isolated by reverse transcription-PCR from human brain cerebellum and several cancer cell lines. The expression of VLP transcript is most abundant in the placenta. Like VHL, VLP contains a beta domain capable of binding HIFalpha. However, unlike VHL, it does not contain a recognizable alpha domain, which is required for nucleating the multiprotein E3 ubiquitin ligase complex. The increased expression of VLP in the presence of VHL attenuated the ubiquitination of HIFalpha and led to the accumulation of downstream HIF target genes. These results taken together indicate that VLP functions as a dominant-negative VHL to serve as a protector of HIFalpha. PMID:14757845

  17. Charakterisierung von Scp160p, einem mRNA- und ribosomenassoziierten Protein in Saccharomyces cerevisiae

    OpenAIRE

    Frey, Steffen

    2002-01-01

    Die Mitglieder der konservierten Vigilin-Proteinfamilie zeichnet sich durch ihren modularen Aufbau aus 14-15 KH-Domänen aus, die eine RNA-Bindung vermitteln können. Aufgrund von Untersuchungen in unterschiedlichen eukaryontischen Modellsystemen wurde ihnen eine Bedeutung in verschiedenen funktionellen Zusammen-hängen wie Zellaktivierung, Proteinsynthese und -Sekretion, mRNA- und tRNA-Metabolismus, Funktion von Heterochromatin sowie Teilung von ER- und Kernmembranen zugeordnet. Ziel dieser Ar...

  18. Synthese von thermoplastisch verarbeitbaren Fettsäure-Acylderivaten der Stärke und Proteine

    OpenAIRE

    Winkler, Henning

    2014-01-01

    In den vergangenen Jahren wurden stetig wachsende Produktionskapazitäten von Biokunststoffen aus nachwachsenden Rohstoffe nverzeichnet. Trotz großer Produktionskapazitäten und einem geeigneten Eigenschaftsprofil findet Stärke nur als hydrophile, mit Weichmachern verarbeitete thermoplastische Stärke (TPS) in Form von Blends mit z. B. Polyestern Anwendung. Gleiches gilt für Kunststoffe auf Proteinbasis. Die vorliegende Arbeit hat die Entwicklung von Biokunststoffen auf Stärkebasis zum Ziel, wel...

  19. Comparison of Proteins in Whole Blood and Dried Blood Spot Samples by LC/MS/MS

    Science.gov (United States)

    Chambers, Andrew G.; Percy, Andrew J.; Hardie, Darryl B.; Borchers, Christoph H.

    2013-09-01

    Dried blood spot (DBS) sampling methods are desirable for population-wide biomarker screening programs because of their ease of collection, transportation, and storage. Immunoassays are traditionally used to quantify endogenous proteins in these samples but require a separate assay for each protein. Recently, targeted mass spectrometry (MS) has been proposed for generating highly-multiplexed assays for biomarker proteins in DBS samples. In this work, we report the first comparison of proteins in whole blood and DBS samples using an untargeted MS approach. The average number of proteins identified in undepleted whole blood and DBS samples by liquid chromatography (LC)/MS/MS was 223 and 253, respectively. Protein identification repeatability was between 77 %-92 % within replicates and the majority of these repeated proteins (70 %) were observed in both sample formats. Proteins exclusively identified in the liquid or dried fluid spot format were unbiased based on their molecular weight, isoelectric point, aliphatic index, and grand average hydrophobicity. In addition, we extended this comparison to include proteins in matching plasma and serum samples with their dried fluid spot equivalents, dried plasma spot (DPS), and dried serum spot (DSS). This work begins to define the accessibility of endogenous proteins in dried fluid spot samples for analysis by MS and is useful in evaluating the scope of this new approach.

  20. Supramolecular Structures with Blood Plasma Proteins, Sugars and Nanosilica

    Science.gov (United States)

    Turov, V. V.; Gun'ko, V. M.; Galagan, N. P.; Rugal, A. A.; Barvinchenko, V. M.; Gorbyk, P. P.

    Supramolecular structures with blood plasma proteins (albumin, immunoglobulin and fibrinogen (HPF)), protein/water/silica and protein/water/ silica/sugar (glucose, fructose and saccharose) were studied by NMR, adsorption, IR and UV spectroscopy methods. Hydration parameters, amounts of weakly and strongly bound waters and interfacial energy (γ S) were determined over a wide range of component concentrations. The γ S(C protein,C silica) graphs were used to estimate the energy of protein-protein, protein-surface and particle-particle interactions. It was shown that interfacial energy of self-association (γ as) of protein molecules depends on a type of proteins. A large fraction of water bound to proteins can be displaced by sugars, and the effect of disaccharide (saccharose) was greater than that of monosugars. Changes in the structural parameters of cavities in HPF molecules and complexes with HPF/silica nanoparticles filled by bound water were analysed using NMR-cryoporometry showing that interaction of proteins with silica leads to a significant decrease in the amounts of water bound to both protein and silica surfaces. Bionanocomposites with BSA/nanosilica/sugar can be used to influence states of living cells and tissues after cryopreservation or other treatments. It was shown that interaction of proteins with silica leads to strong decrease in the volume of all types of internal cavities filled by water.

  1. Molecular interactions of graphene oxide with human blood plasma proteins

    Science.gov (United States)

    Kenry, Affa Affb Affc; Loh, Kian Ping; Lim, Chwee Teck

    2016-04-01

    We investigate the molecular interactions between graphene oxide (GO) and human blood plasma proteins. To gain an insight into the bio-physico-chemical activity of GO in biological and biomedical applications, we performed a series of biophysical assays to quantify the molecular interactions between GO with different lateral size distributions and the three essential human blood plasma proteins. We elucidate the various aspects of the GO-protein interactions, particularly, the adsorption, binding kinetics and equilibrium, and conformational stability, through determination of quantitative parameters, such as GO-protein association constants, binding cooperativity, and the binding-driven protein structural changes. We demonstrate that the molecular interactions between GO and plasma proteins are significantly dependent on the lateral size distribution and mean lateral sizes of the GO nanosheets and their subtle variations may markedly influence the GO-protein interactions. Consequently, we propose the existence of size-dependent molecular interactions between GO nanosheets and plasma proteins, and importantly, the presence of specific critical mean lateral sizes of GO nanosheets in achieving very high association and fluorescence quenching efficiency of the plasma proteins. We anticipate that this work will provide a basis for the design of graphene-based and other related nanomaterials for a plethora of biological and biomedical applications.

  2. Analysis of the complexity of the multimeric structure of factor VIII related antigen/von Willebrand protein using a modified electrophoretic technique.

    OpenAIRE

    Enayat, M S; Hill, F G

    1983-01-01

    A method for multimeric analysis of factor VIII related antigen/von Willebrand protein is described. By modifying an existing method the technique has been simplified and optimised so that the different molecular forms of factor VIII related antigen and their triplet structure can be visualised. Clear differences can be seen between patterns in normals and type IIA von Willebrand's disease variants in that the latter lack high and intermediate multimers and also have a different configuration...

  3. The von Hippel-Lindau protein sensitizes renal carcinoma cells to apoptotic stimuli through stabilization of BIMEL

    OpenAIRE

    Guo, Y; Schoell, MC; Freeman, RS

    2009-01-01

    von Hippel-Lindau (VHL) disease is caused by germ-line mutations in the VHL tumor suppressor gene and is the most common cause of inherited renal cell carcinoma (RCC). Mutations in the VHL gene also occur in a large majority of sporadic cases of clear-cell RCC, which have high intrinsic resistance to chemotherapy and radiotherapy. Here we show that VHL-deficient RCC cells express lower levels of the pro-apoptotic Bcl-2 family protein BIMEL and are more resistant to etoposide and UV radiation ...

  4. Dogs with hearth diseases causing turbulent high-velocity blood flow have changes in patelet function and von Willebrand factor multimer distribution

    DEFF Research Database (Denmark)

    Tarnow, Inge; Kristensen, Annemarie Thuri; Olsen, Lisbeth Høier; Falk, Bo Torkel; Haubro, Lotte; Pedersen, Lotte Gam; Pedersen, Henrik Duelund

    2005-01-01

    echocardiography were performed in all dogs. PFA100 closure times (the ability of platelets to occlude a hole in a membrane at high shear rates), platelet activation markers (plasma thromboxane B2 concentration, platelet surface P-selectin expression), platelet aggregation (in whole blood and platelet-rich plasma......The purpose of this prospective study was to investigate platelet function using in vitro tests based on both high and low shear rates and von Willebrand factor (vWf) multimeric composition in dogs with cardiac disease and turbulent high-velocity blood flow. Client-owned asymptomatic, untreated...... with 3 different agonists), and vWf multimers were analyzed. Cavaliers with moderate to severe MR and dogs with SAS had longer closure times and a lower percentage of the largest vWf multimers than did controls. Maximal aggregation responses were unchanged in dogs with SAS but enhaned in Cavaliers with...

  5. The spider hemolymph clot proteome reveals high concentrations of hemocyanin and von Willebrand factor-like proteins.

    Science.gov (United States)

    Sanggaard, Kristian W; Dyrlund, Thomas F; Bechsgaard, Jesper S; Scavenius, Carsten; Wang, Tobias; Bilde, Trine; Enghild, Jan J

    2016-02-01

    Arthropods include chelicerates, crustaceans, and insects that all have open circulation systems and thus require different properties of their coagulation system than vertebrates. Although the clotting reaction in the chelicerate horseshoe crab (Family: Limulidae) has been described in details, the overall protein composition of the resulting clot has not been analyzed for any of the chelicerates. The largest class among the chelicerates is the arachnids, which includes spiders, ticks, mites, and scorpions. Here, we use a mass spectrometry-based approach to characterize the spider hemolymph clot proteome from the Brazilian whiteknee tarantula, Acanthoscurria geniculata. We focused on the insoluble part of the clot and demonstrated high concentrations of proteins homologous to the hemostasis-related and multimerization-prone von Willebrand factor. These proteins, which include hemolectins and vitellogenin homologous, were previously identified as essential components of the hemolymph clot in crustaceans and insects. Their presence in the spider hemolymph clot suggests that the origin of these proteins' function in coagulation predates the split between chelicerates and mandibulata. The clot proteome reveals that the major proteinaceous component is the oxygen-transporting and phenoloxidase-displaying abundant hemolymph protein hemocyanin, suggesting that this protein also plays a role in clot biology. Furthermore, quantification of the peptidome after coagulation revealed the simultaneous activation of both the innate immune system and the coagulation system. In general, many of the identified clot-proteins are related to the innate immune system, and our results support the previously suggested crosstalk between immunity and coagulation in arthropods. PMID:26621385

  6. Blood plasma proteins and protein fractions in roe deer Capreolus capreolus L.

    Directory of Open Access Journals (Sweden)

    Dorota CYGAN-SZCZEGIELNIAK

    2015-09-01

    Full Text Available The aim of the research was to investigate some selected biochemical blood parameters in roe deer (Capreolus capreolus L.. The experiment covered 15 from 2 to 3-year-old bucks from Kuyavian-Pomeranian Voivodeship. The animals were shot by individual hunters on the shooting grounds during the hunting season of 2008/2009 (in the accordance with the Journal of Laws No 48. The material for the research was blood plasma obtained after centrifuging full, nonhemolyzed blood. The blood was collected from the zygomatic vein directly to the test tubes with EDTA and transported in cooling conditions to the laboratory. After transporting the samples of blood to a certified analytical laboratory, the following elements of the obtained blood plasma were examined: ceruloplasmin . using turbidimetric method; transferrin . using immunoturbimetric method; troponin- using a third generation assay on an Elecsys; total protein, albumin, globulin . using spectrophotometric method and total iron . using colorimetric method. The results were statistically analyzed, i.e. the correlation between the parameters was measured by means of Pearsonfs correlation coefficient. The analysis of the results revealed a number of statistically significant relations between the parameters under the investigation, especially among the compounds directly responsible for metabolism of iron and copper. A statistically important positive correlation was observed between ceruloplasmin and ferritin (r = 0.563; P.0.05 and a negative one between transferrin and troponin (r = -0.609; P.0.05. Moreover, the content of transferrin . an iron-binding protein . was 0.17 g/l, while the concentration of iron was 58 ƒĘmol/l. The content of ceruloplasmin . a protein responsible for metabolism of copper . was very low (0.036 g/l. The level of proteins in the blood plasma of the animals under the research was approximately 72 g/l, with the share of albumins about 46%. The albumin-globulin ratio was 0.86.

  7. Toxicity of Bacillus thuringiensis (L.) Cry proteins against summer fruit tortrix (Adoxophyes orana - Fischer von Rösslerstamm).

    Science.gov (United States)

    Radosavljevic, Jelena; Naimov, Samir

    2016-07-01

    The activity of seven Cry1, one Cry9 and one hybrid Cry1 protoxins against neonate larvae of summer fruit tortrix (Adoxophyes orana - Fischer von Rösslerstamm) has been investigated. Cry1Ia is identified as the most toxic protein, followed by Cry1Aa and Cry1Ac. Cry1Ca, Cry1Cb, Cry1Da and Cry1Fa were less active, while SN19 (Cry1 hybrid protein with domain composition 1Ba/1Ia/1Ba) and Cry9Aa exhibited negligible toxicity against A. orana. In vitro trypsin-activated Cry1Ac is still less active than Cry1Ia protoxin, suggesting that toxicity of Cry1Ia is most probably due to more complex differences in further downstream processing, toxin-receptor interactions and pore formation in A. orana's midgut epithelium. PMID:27311897

  8. Studien zum Verhalten von Anthocyanen aus Heidelbeeren im Humanstoffwechsel - Stabilisierung und Bindung durch Proteine

    OpenAIRE

    Ackermann, Matthias

    2010-01-01

    Identifizierung und Strukturaufklärung von Anthocyanen und ihrer Metabolite erfolgten mit Hilfe der mittels Hochleistungsflüssigchromatographie-Diodenarray-Detektion-Elektro-spray-Tan¬dem¬massen¬spektrometrie (HPLC-DAD-ESI-MS/MS). Quantitative Analysen wurden via HPLC-DAD durchgeführt. Die hierzu erforderlichen Referenzverbindungen wurden mittels präparativer HPLC aus Heidelbeeren isoliert (Reinheit zwischen 85,8% und 99,4%). Der Gehalt an Anthocyanen in den untersuchten Heidelbeerfrüchten la...

  9. Untersuchungen zum Protein-Koimport von Katalase A in Peroxisomen und Mitochondrien in der Hefe Saccharomyces cerevisiae

    OpenAIRE

    Drescher, Diane

    2006-01-01

    Ziel der vorliegenden Arbeit war die Untersuchung des dualen Targetings von Katalase A in Peroxisomen und Mitochondrien der Hefe S. cerevisiae. Mit Hilfe einer CTA-GFP-Fusion, in der GFP am Carboxyterminus von Cta1p vorliegt, wurden sowohl mit immunochemischen und fluoreszenzmikroskopischen Nachweismethoden, als auch mit Enzymaktivitätstests Hinweise auf eine mitochondriale Lokalisation von Cta1p erhalten. Die Beeinflussung der Zielsteuerung von Cta1p in der Zelle durch GFP, welches das P...

  10. The von Hippel-Lindau protein sensitizes renal carcinoma cells to apoptotic stimuli through stabilization of BIMEL

    Science.gov (United States)

    Guo, Y; Schoell, MC; Freeman, RS

    2009-01-01

    von Hippel-Lindau (VHL) disease is caused by germ-line mutations in the VHL tumor suppressor gene and is the most common cause of inherited renal cell carcinoma (RCC). Mutations in the VHL gene also occur in a large majority of sporadic cases of clear-cell RCC, which have high intrinsic resistance to chemotherapy and radiotherapy. Here we show that VHL-deficient RCC cells express lower levels of the pro-apoptotic Bcl-2 family protein BIMEL and are more resistant to etoposide and UV radiation induced death compared to the same cells stably expressing the wild type VHL protein (pVHL). Re-introducing pVHL into VHL-null cells increased the half-life of BIMEL protein without affecting its mRNA expression, and over-expressing pVHL inhibited BIMEL polyubiquitination. Suppressing pVHL expression with RNA interference resulted in a decrease in BIMEL protein and a corresponding decrease in the sensitivity of RCC cells to apoptotic stimuli. Directly inhibiting BIMEL expression in pVHL-expressing RCC cells caused a similar decrease in cell death. These results demonstrate that pVHL acts to promote BIMEL protein stability in RCC cells, and that destabilization of BIMEL in the absence of pVHL contributes to the increased resistance of VHL-null RCC cells to certain apoptotic stimuli. PMID:19305426

  11. The von Hippel-Lindau protein sensitizes renal carcinoma cells to apoptotic stimuli through stabilization of BIM(EL).

    Science.gov (United States)

    Guo, Y; Schoell, M C; Freeman, R S

    2009-04-23

    von Hippel-Lindau (VHL) disease is caused by germ-line mutations in the VHL tumor suppressor gene and is the most common cause of inherited renal cell carcinoma (RCC). Mutations in the VHL gene also occur in a large majority of sporadic cases of clear-cell RCC, which have high intrinsic resistance to chemotherapy and radiotherapy. Here we show that VHL-deficient RCC cells express lower levels of the proapoptotic Bcl-2 family protein BIM(EL) and are more resistant to etoposide and UV radiation-induced death compared to the same cells stably expressing the wild-type VHL protein (pVHL). Reintroducing pVHL into VHL-null cells increased the half-life of BIM(EL) protein without affecting its mRNA expression, and overexpressing pVHL inhibited BIM(EL) polyubiquitination. Suppressing pVHL expression with RNA interference resulted in a decrease in BIM(EL) protein and a corresponding decrease in the sensitivity of RCC cells to apoptotic stimuli. Directly inhibiting BIM(EL) expression in pVHL-expressing RCC cells caused a similar decrease in cell death. These results demonstrate that pVHL acts to promote BIM(EL) protein stability in RCC cells, and that destabilization of BIM(EL) in the absence of pVHL contributes to the increased resistance of VHL-null RCC cells to certain apoptotic stimuli. PMID:19305426

  12. Pharmaceutical protein production by yeast: towards production of human blood proteins by microbial fermentation

    DEFF Research Database (Denmark)

    Martínez, José L; Liu, Lifang; Petranovic, Dina;

    2012-01-01

    Since the approval of recombinant insulin from Escherichia coli for its clinical use in the early 1980s, the amount of recombinant pharmaceutical proteins obtained by microbial fermentations has significantly increased. The recent advances in genomics together with high throughput analysis...... techniques (the so-called—omics approaches) and integrative approaches (systems biology) allow the development of novel microbial cell factories as valuable platforms for large scale production of therapeutic proteins. This review summarizes the main achievements and the current situation in the field of...... recombinant therapeutics using yeast Saccharomyces cerevisiae as a model platform, and discusses the future potential of this platform for production of blood proteins and substitutes....

  13. Transactivating-transduction protein-polyethylene glycol modified liposomes traverse the blood-spinal cord and blood-brain barriers

    Institute of Scientific and Technical Information of China (English)

    Xianhu Zhou; Chunyuan Wang; Shiqing Feng; Jin Chang; Xiaohong Kong; Yang Liu; Shijie Gao

    2012-01-01

    Naive liposomes can cross the blood-brain barrier and blood-spinal cord barrier in small amounts. Liposomes modified by a transactivating-transduction protein can deliver antibiotics for the treatment of acute bacterial infection-induced brain inflammation. Liposomes conjugated with polyethylene glycol have the capability of long-term circulation. In this study we prepared transactivating-transduction protein-polyethylene glycol-modified liposomes labeled with fluorescein isothiocyanate. Thus, liposomes were characterized by transmembrane, long-term circulation and fluorescence tracing. Uptake, cytotoxicity, and the ability of traversing blood-spinal cord and blood-brain barriers were observed following coculture with human breast adenocarcinoma cells (MCF-7). Results demonstrated that the liposomes had good biocompatibility, and low cytotoxicity when cocultured with human breast adenocarcinoma cells. Liposomes could traverse cell membranes and entered the central nervous system and neurocytes through the blood-spinal cord and blood-brain barriers of rats via the systemic circulation. These results verified that fluorescein isothiocyanate-modified transactivating-transduction protein-polyethylene glycol liposomes have the ability to traverse the blood-spinal cord and blood-brain barriers.

  14. Analysis of von Willebrand factor A domain-related protein (WARP polymorphism in temperate and tropical Plasmodium vivax field isolates

    Directory of Open Access Journals (Sweden)

    Zakeri Sedigheh

    2009-06-01

    Full Text Available Abstract Background The identification of key molecules is crucial for designing transmission-blocking vaccines (TBVs, among those ookinete micronemal proteins are candidate as a general class of malaria transmission-blocking targets. Here, the sequence analysis of an extra-cellular malaria protein expressed in ookinetes, named von Willebrand factor A domain-related protein (WARP, is reported in 91 Plasmodium vivax isolates circulating in different regions of Iran. Methods Clinical isolates were collected from north temperate and southern tropical regions in Iran. Primers have been designed based on P. vivax sequence (ctg_6991 which amplified a fragment of about 1044 bp with no size variation. Direct sequencing of PCR products was used to determine polymorphism and further bioinformatics analysis in P. vivax sexual stage antigen, pvwarp. Results Amplified pvwarp gene showed 886 bp in size, with no intron. BLAST analysis showed a similarity of 98–100% to P. vivax Sal-I strain; however, Iranian isolates had 2 bp mismatches in 247 and 531 positions that were non-synonymous substitution [T (ACT to A (GCT and R (AGA to S (AGT] in comparison with the Sal-I sequence. Conclusion This study presents the first large-scale survey on pvwarp polymorphism in the world, which provides baseline data for developing WARP-based TBV against both temperate and tropical P. vivax isolates.

  15. Charakterisierung des viszeralen Fettes als Quelle von Adipozytokinen und Untersuchungen zur Funktion dieser Proteine im Kontext von Typ 2 Diabetes mellitus

    OpenAIRE

    Weigert, Johanna

    2010-01-01

    Die Adipositas, insbesondere das Vorliegen einer viszeralen Adipositas, ist mit einem erhöhten Risiko für metabolische und kardiovaskuläre Erkrankungen verbunden. Das Ziel dieser Arbeit war es, durch einen Vergleich von gepaarten Proben humanen subkutanen und viszeralen Fettgewebes neue Adipozytokine, welche vermehrt vom viszeralen Fettgewebe synthetisiert werden, zu identifizieren und anschließend genauer zu charakterisieren. Hierfür wurden gepaarte humane subkutane und viszerale Fettgewe...

  16. Protein-associated water and secondary structure effect removal of blood proteins from metallic substrates.

    Science.gov (United States)

    Anand, Gaurav; Zhang, Fuming; Linhardt, Robert J; Belfort, Georges

    2011-03-01

    Removing adsorbed protein from metals has significant health and industrial consequences. There are numerous protein-adsorption studies using model self-assembled monolayers or polymeric substrates but hardly any high-resolution measurements of adsorption and removal of proteins on industrially relevant transition metals. Surgeons and ship owners desire clean metal surfaces to reduce transmission of disease via surgical instruments and minimize surface fouling (to reduce friction and corrosion), respectively. A major finding of this work is that, besides hydrophobic interaction adhesion energy, water content in an adsorbed protein layer and secondary structure of proteins determined the access and hence ability to remove adsorbed proteins from metal surfaces with a strong alkaline-surfactant solution (NaOH and 5 mg/mL SDS in PBS at pH 11). This is demonstrated with three blood proteins (bovine serum albumin, immunoglobulin, and fibrinogen) and four transition metal substrates and stainless steel (platinum (Pt), gold (Au), tungsten (W), titanium (Ti), and 316 grade stainless steel (SS)). All the metallic substrates were checked for chemical contaminations like carbon and sulfur and were characterized using X-ray photoelectron spectroscopy (XPS). While Pt and Au surfaces were oxide-free (fairly inert elements), W, Ti, and SS substrates were associated with native oxide. Difference measurements between a quartz crystal microbalance with dissipation (QCM-D) and surface plasmon resonance spectroscopy (SPR) provided a measure of the water content in the protein-adsorbed layers. Hydrophobic adhesion forces, obtained with atomic force microscopy, between the proteins and the metals correlated with the amount of the adsorbed protein-water complex. Thus, the amount of protein adsorbed decreased with Pt, Au, W, Ti and SS, in this order. Neither sessile contact angle nor surface roughness of the metal substrates was useful as predictors here. All three globular proteins

  17. Detection of protein biomarker using a blood glucose meter.

    Science.gov (United States)

    Lan, Tian; Xiang, Yu; Lu, Yi

    2015-01-01

    mHeath technologies are recognized to play important roles in the future of personal care and medicine. However, their full potentials have not been reached, as most of current technologies are restricted to monitoring physical and behavioral parameters, such as body temperature, heart rate, blood pressure, and physical movement, while direct monitoring of biomarkers in body fluids can provide much more accurate and useful information for medical diagnostics. A major barrier to realizing the full potential of mHealth is the high costs and long cycles of developing mHealth devices capable of monitoring biomarkers in body fluids. To lower the costs and shorten the developmental cycle, we have demonstrated the leveraging of the most successful portable medical monitoring device on the market, the blood glucose meter (BGM), with FDA-approved smartphone technologies that allow for wireless transmission and remote monitoring of a wide range of non-glucose targets. In this protocol, an aptamer-based assay for quantification of interferon-γ (IFN-γ) using an off-the-shelf BGM is described. In this assay, an aptamer-based target recognition system is employed. When IFN-γ binds to the aptamer, it triggers the release of a reporter enzyme, invertase, which can catalyze the conversion of sucrose (not detected by BGM) to glucose. The glucose being produced is then detected using a BGM. The system mimics a competitive enzyme-linked immunosorbent assay (ELISA), where the traditional immunoassay is replaced by an aptamer binding assay; the reporter protein is replaced by invertase, and finally the optical or fluorescence detector is replaced with widely available BGMs. PMID:25626534

  18. Enhancement of heat transfer in cryopreservation of red blood cells in liquid nitrogen; Steigerung des Waermeuebergangs bei der Kryokonservierung von roten Blutkoerperchen in fluessigem Stickstoff

    Energy Technology Data Exchange (ETDEWEB)

    Heschel, I. [Technische Hochschule Aachen (Germany). Helmholtz-Inst. fuer Biomedizinische Technik; Mingers, B. [Technische Hochschule Aachen (Germany). Helmholtz-Inst. fuer Biomedizinische Technik; Rau, G. [Technische Hochschule Aachen (Germany). Helmholtz-Inst. fuer Biomedizinische Technik

    1994-12-31

    Development work in cryopreservation of human erythrocytes (red blood cells) is currently aimed at storing the cells recovered from the full-blood conserve in one freezing bag instead of two as formerly required. The doubling of sample thickness entailed in this has rendered the old preservation method insufficient for achieving the requisite cooling rate, this leading to low cell survival rates. In this context the present paper deals with ways of enhancing heat transfer through conditioning of the liquid nitrogen and modification of the cryopreservation method. Using the results of experimental studies it shows that these measures make it possible to achieve the requisite minimum cooling rate also in samples of double thickness. (orig.) [Deutsch] Bei der Kryokonservierung von humanen Erythrozyten (roten Blutkoerperchen) wird angestrebt, die aus einer Vollblutkonserve gewonnenen Zellen in einem statt bislang zwei zugelassenen Einfrierbeuteln konservieren zu koennen. Die damit verbundene Verdopplung der Probenschichtdicke fuehrt dazu, dass die hohen erforderlichen Kuehlraten bei Verwendung des bislang ueblichen Konservierungsverfahrens nicht realisiert werden koennen. Dementsprechend werden nur geringere Zellueberlebensraten erzielt. Der Beitrag befasst sich daher mit den Moeglichkeiten zur Steigerung des Waermeuebergangs, die sich durch eine Konditionierung des fluessigen Stickstoffs und Modifikationen beim Kryokonservierungsverfahren ergeben. Anhand der Ergebnisse experimenteller Untersuchungen wird schliesslich gezeigt, dass durch die getroffenen Massnahmen auch bei verdoppelter Probenschichtdicke die mindestens erforderlichen Kuehlraten erzielt werden koennen. (orig.)

  19. Enhancement of heat transfer in cryopreservation of red blood cells in liquid nitrogen; Steigerung des Waermeuebergangs bei der Kryokonservierung von roten Blutkoerperchen in fluessigem Stickstoff

    Energy Technology Data Exchange (ETDEWEB)

    Heschel, I. [Helmholtz-Inst. fuer Biomedizinische Technik an der RWTH Aachen (Germany); Mingers, B. [Helmholtz-Inst. fuer Biomedizinische Technik an der RWTH Aachen (Germany); Rau, G. [Helmholtz-Inst. fuer Biomedizinische Technik an der RWTH Aachen (Germany)

    1994-12-31

    Development work in cryopreservation of human crythrocytes (red blood cells) is currently aimed at storing the cells recovered from the full-blood conserve in one freezing bag instead of two as formerly required. The doubling of sample thickness entailed in this has rendered the old preservation method insufficient for achieving the requisite cooling rate, this leading to low cell survival rates. In this context the present paper deals with ways of enhancing heat transfer through conditioning of the liquid nitrogen and modification of the cryopreservation method. It uses the results of experimental studies to show that these measures make it possible to achieve the requisite minimum cooling rate also in samples of double thickness. (orig.) [Deutsch] Bei der Kryokonservierung von humanen Erythrozyten (roten Blutkoerperchen) wird angestrebt, die aus einer Vollblutkonserve gewonnenen Zellen ine einem statt bislang zwei zugelassenen Einfrierbeuteln konservieren zu koennen. Die damit verbundene Verdopplung der Probenschichtdicke fuehrt dazu, dass die hohen erforderlichen Kuehlraten bei Verwendung des bislang ueblichen Konservierungsverfahrens nicht realisiert werden koennen. Dementsprechend werden nur geringere Zellueberlebensraten erzielt. Der Beitrag befasst sich daher mit den Moeglichkeiten zur Steigerung des Waermeuebergangs, die sich durch eine Konditionierung des fluessigen Stickstoffs und Modifikationen beim Kryokonservierungsverfahren ergeben. Anhand der Ergebnisse experimenteller Untersuchungen wird schliesslich gezeigt, dass durch die getroffenen Massnahmen auch bei verdoppelter Probenschichtdicke die mindestens erforderlichen Kuehlraten erzielt werden koennen. (orig.)

  20. Regulation of blood-testis barrier by actin binding proteins and protein kinases.

    Science.gov (United States)

    Li, Nan; Tang, Elizabeth I; Cheng, C Yan

    2016-03-01

    The blood-testis barrier (BTB) is an important ultrastructure in the testis, since the onset of meiosis and spermiogenesis coincides with the establishment of a functional barrier in rodents and humans. It is also noted that a delay in the assembly of a functional BTB following treatment of neonatal rats with drugs such as diethylstilbestrol or adjudin also delays the first wave of spermiation. While the BTB is one of the tightest blood-tissue barriers, it undergoes extensive remodeling, in particular, at stage VIII of the epithelial cycle to facilitate the transport of preleptotene spermatocytes connected in clones across the immunological barrier. Without this timely transport of preleptotene spermatocytes derived from type B spermatogonia, meiosis will be arrested, causing aspermatogenesis. Yet the biology and regulation of the BTB remains largely unexplored since the morphological studies in the 1970s. Recent studies, however, have shed new light on the biology of the BTB. Herein, we critically evaluate some of these findings, illustrating that the Sertoli cell BTB is regulated by actin-binding proteins (ABPs), likely supported by non-receptor protein kinases, to modulate the organization of actin microfilament bundles at the site. Furthermore, microtubule-based cytoskeleton is also working in concert with the actin-based cytoskeleton to confer BTB dynamics. This timely review provides an update on the unique biology and regulation of the BTB based on the latest findings in the field, focusing on the role of ABPs and non-receptor protein kinases. PMID:26628556

  1. Direct observation of interaction between proteins and blood-compatible polymer surfaces.

    Science.gov (United States)

    Hayashi, Tomohiro; Tanaka, Masaru; Yamamoto, Sadaaki; Shimomura, Masatsugu; Hara, Masahiko

    2007-12-01

    The adhesion force between blood-compatible polymer (poly(2-methoxyethyl acrylate: PMEA) and proteins (fibrinogen and bovine serum albumin (BSA)) were measured by atomic force microscopy. The PMEA surface showed almost no adhesion to native protein molecules, whereas non-blood-compatible poly(n-butyl acrylate): PBA strongly adhered to proteins. Interestingly, adhesion did appear between PMEA and proteins when the proteins were denatured. In all cases, these trends were not affected by the conditions of the solution. Combining the results with previous reports, the authors conclude that interfacial water molecules play a critical role in the protein resistance of PMEA. PMID:20408647

  2. von Hippel-Lindau protein regulates transition from fetal to adult circulatory system in retina

    OpenAIRE

    Kurihara, Toshihide; Kubota, Yoshiaki; Ozawa, Yoko; Takubo, Keiyo; Noda, Kousuke; Simon, M. Celeste; Johnson, Randall S; Suematsu, Makoto; Tsubota, Kazuo; Ishida, Susumu; Goda, Nobuhito; Suda, Toshio; Okano, Hideyuki

    2010-01-01

    In the early neonatal stage, the fetal circulatory system undergoes dramatic transition to the adult circulatory system. Normally, embryonic connecting vessels such as ductus arteriosus and the foramen ovale close and regress. In the neonatal retina, hyaloid vessels maintaining blood flow in the embryonic retina regress, and retinal vessels take over to form adult-type circulatory system. This process is regulated by the programmed cell death switch mediated by macrophages via Wnt and Angiopo...

  3. Surfactant protein D levels in umbilical cord blood and capillary blood of premature infants. The influence of perinatal factors

    DEFF Research Database (Denmark)

    Dahl, Marianne; Holmskov, Uffe; Husby, Steffen;

    2006-01-01

    Surfactant protein D (SP-D) is a collectin that plays an important role in the innate immune system and takes part in the surfactant homeostasis by regulating the surfactant pool size. The aims of this study were to investigate the values of SP-D in umbilical cord blood and capillary blood of...... premature infants and to relate the levels to perinatal conditions. A total of 254 premature infants were enrolled in the present study. Umbilical cord blood was drawn at the time of birth and capillary blood at regular intervals throughout the admission. The concentration of SP-D in umbilical cord blood...... concentration of SP-D in capillary blood day 1 was 1,466 ng/mL (range 410-5,051 ng/mL), with lowest values in infants born with ROM and delivered vaginally. High SP-D levels in umbilical cord blood and capillary blood on day 1 were found to be more likely in infants in need for respiratory support or surfactant...

  4. Limited polymorphism in Plasmodium falciparum ookinete surface antigen, von Willebrand factor A domain-related protein from clinical isolates

    Directory of Open Access Journals (Sweden)

    Eisen Damon P

    2006-07-01

    Full Text Available Abstract Background As malaria becomes increasingly drug resistant and more costly to treat, there is increasing urgency to develop effective vaccines. In comparison to other stages of the malaria lifecycle, sexual stage antigens are under less immune selection pressure and hence are likely to have limited antigenic diversity. Methods Clinical isolates from a wide range of geographical regions were collected. Direct sequencing of PCR products was then used to determine the extent of polymorphisms for the novel Plasmodium falciparum sexual stage antigen von Willebrand Factor A domain-related Protein (PfWARP. These isolates were also used to confirm the extent of diversity of sexual stage antigen Pfs28. Results PfWARP was shown to have non-synonymous substitutions at 3 positions and Pfs28 was confirmed to have a single non-synonymous substitution as previously described. Conclusion This study demonstrates the limited antigenic diversity of two prospective P. falciparum sexual stage antigens, PfWARP and Pfs28. This provides further encouragement for the proceeding with vaccine trials based on these antigens.

  5. Regulation of homeostasis in the process of protein absorption from small intestine to blood

    OpenAIRE

    Akmal Yuldashev; Ravshan Rahmanov; Mukaddas Rahmatova; Margarita Tarinova; Aziza Nishanova; Gulnara Islamova

    2010-01-01

    Electron microscopic and immunоfluorescent study in rats aged 1 and 3 days after birth allowed to establish a process of absorption of protein from the small intestine into the lymph and blood. Blood homeostasis was provided by the proteins filtrated from glomerular capillaries of nephrons and reabsorbed by the epithelial cells in canaliculi of nephrons. The absorbed natural heterologous protein was depleted by lysosomes of epithelial cells of intestine and kidneys and macrophages. It support...

  6. The in vitro antioxidant properties of alcalase hydrolysate prepared from silkie fowl (Gallus gallus) blood protein.

    Science.gov (United States)

    Cheng, Fu-Yuan; Lai, I-Chun; Lin, Liang-Chuan; Sakata, Ryoichi

    2016-07-01

    Two types of proteins including blood plasma protein and blood cell protein were isolated from silkie fowl (Gallus gallus) blood and hydrolyzed using alcalase for 0, 2, 4 and 6 h. The blood plasma protein hydrolysate (BPH) and blood cell protein hydrolysate (BCH) were analyzed for pH value, peptide content and antioxidative properties. The significantly higher peptide contents were observed in BPH than that of BCH, which showed that blood plasma protein was more suitable to hydrolysis by alcalase than blood cell protein. Both BPH and BCH showed strong 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical-scavenging activity and Fe(2+) chelating ability. BPH at 4 h of hydrolysis (BPH4) demonstrated significantly higher antioxidant capacity than those treated by alcalase in most of the assays. The BPH4 was separated using ultra-filtration and assessment of the fractions and indicated that low molecular weight of peptides (< 3 kDa) possessed greater DPPH scavenging activity, Fe(2+) chelating ability and inhibitory activity of lipid peroxidation. These results show that BPH has the potential to be ingredients in the food industry as a replacement of synthetic antioxidants. PMID:26556592

  7. Identifizierung regulatorischer Bereiche im E1B-55kDa Protein von Adenovirus Serotyp 5 und ihre Rolle bei der Transformation

    OpenAIRE

    Endter, Christian

    2002-01-01

    Das E1B-55kDa-Protein von Adenovirus Typ 5 ist ein multifunktionelles Polypeptid, dessen onkogenen Aktivitäten verschiedene Wirkmechanismen zugrunde liegen, die zumindest teilweise über die Modulation des Tumorsuppressorproteins p53 vermittelt werden. Im Rahmen dieser Arbeit wurde die Bedeutung des Exportsignals und anderer Domänen für die transformierenden Eigenschaften des E1B-55kDa-Proteins untersucht. Dazu wurden Punktmutationen innerhalb der NES-Region und in benachbarte Bereiche des E1B...

  8. Die Rolle von growth arrest specific protein 6 im Aldosteron induzierten Endorganschaden

    OpenAIRE

    Theuer, Stefanie

    2013-01-01

    Growth arrest specific protein 6 (Gas 6) is involved in inflammatory kidney diseases, vascular remodeling, cell adhesion, and thrombus formation. We explored a role for Gas 6 in aldosterone-induced target organ damage. We observed that Gas 6 was upregulated in rats with high aldosterone levels. Mineralocorticoid receptor blockade prevented target organ damage and decreased the elevated Gas 6 expression. Vascular smooth muscle cells given aldosterone increased their Gas 6 expression in vitro. ...

  9. Blood profiling of proteins and steroids during weight maintenance with manipulation of dietary protein level and glycaemic index

    DEFF Research Database (Denmark)

    Wang, Ping; Holst, Claus; Astrup, Arne;

    2012-01-01

    ), evenly selected from four dietary groups that varied in protein and GI levels. The blood concentrations of twenty-nine proteins and three steroid hormones were measured. The changes in analytes during weight maintenance largely correlated negatively with the changes during weight loss, with some...

  10. The plasma protein fibrinogen stabilizes clusters of red blood cells in microcapillary flows

    OpenAIRE

    Brust, Matthias,; Aouane, Othmane; Thiébaud, Marine; Flormann, Daniel; Verdier, Claude; Kaestner, Lars; Laschke, Matthias; Selmi, Hassib; Benyoussef, Abdellilah; Podgorski, Thomas; Coupier, Gwennou; Misbah, Chaouqi; Wagner, Christian

    2014-01-01

    The supply of oxygen and nutrients and the disposal of metabolic waste in the organs depend strongly on how blood, especially red blood cells, flow through the microvascular network. Macromolecular plasma proteins such as fibrinogen cause red blood cells to form large aggregates, called rouleaux, which are usually assumed to be disaggregated in the circulation due to the shear forces present in bulk flow. This leads to the assumption that rouleaux formation is only relevant in the venule netw...

  11. INFLUENCE OF NATURAL IMMUNOMODULATORS ON PROTEIN FRACTIONS AND CORTISOL CONTENT IN RABBIT BLOOD UNDER STRESS

    OpenAIRE

    Grabovskyi S.; Grabovska O.

    2015-01-01

    The results of determination of protein fractions, cortisol content in blood of rabbits, which further added to the feed of natural origin biologically active substances are presented in the article. As an antistressors and immunomodulators in pre-slaughter period are using of spleen extract biologically active substances were obtained with ultrasound application. The purpose of research — determination of changes of protein fractions, cortisol content in rabbits blood before slaughter and th...

  12. Silica Nanoparticles Effects on Blood Coagulation Proteins and Platelets

    OpenAIRE

    Volodymyr Gryshchuk; Natalya Galagan

    2016-01-01

    Interaction of nanoparticles with the blood coagulation is important prior to their using as the drug carriers or therapeutic agents. The aim of present work was studying of the primary effects of silica nanoparticles (SiNPs) on haemostasis in vitro. We studied the effect of SiNPs on blood coagulation directly estimating the activation of prothrombin and factor X and to verify any possible effect of SiNPs on human platelets. It was shown that SiNPs shortened coagulation time in APTT and PT te...

  13. Intake of total protein, plant protein and animal protein in relation to blood pressure : a meta-analysis of observational and intervention studies

    NARCIS (Netherlands)

    Tielemans, S. M. A. J.; Altorf-van der Kuil, W.; Engberink, M. F.; Brink, E. J.; van Baak, M. A.; Bakker, S. J. L.; Geleijnse, J. M.

    2013-01-01

    There is growing evidence from epidemiological studies that dietary protein may beneficially influence blood pressure (BP), but findings are inconclusive. We performed a meta-analysis of 29 observational studies and randomized controlled trials (RCTs) of dietary protein and types of protein in relat

  14. Homocysteine induces production of monocyte chemoattractant protein-1 and interleukin-8 in cultured human whole blood

    Institute of Scientific and Technical Information of China (English)

    Xiao-kun ZENG; Daniel G REMICK; Xian WANG

    2004-01-01

    AIM: To investigate whether increased plasma L-homocysteine (Hcy) level could promote monocyte chemoattractant protein-1 (MCP-1) and interleukin-8 (IL-8) in cultured whole blood. METHODS: Human whole blood or different type of peripheral blood cells from health volunteers were incubated with Hcy and/or the inhibitors. MCP- 1 and IL-8 level were measured by ELISA assay. RESULTS: Hcy 10-1000 μmol/L induced production of MCP-1 and IL-8 in cultured human whole blood (P<0.05). The major cellular source of these chemokines comed from monocytes.Meanwhile,Hcy also promoted the upregulation of MPO level even at the 10 μmol/L in the cultured whole blood.secretion in cultured human whole blood, especially in monocytes via oxidative stress mechanism.

  15. A high confidence, manually validated human blood plasma protein reference set

    DEFF Research Database (Denmark)

    Schenk, Susann; Schoenhals, Gary J; de Souza, Gustavo;

    2008-01-01

    BACKGROUND: The immense diagnostic potential of human plasma has prompted great interest and effort in cataloging its contents, exemplified by the Human Proteome Organization (HUPO) Plasma Proteome Project (PPP) pilot project. Due to challenges in obtaining a reliable blood plasma protein list......, HUPO later re-analysed their own original dataset with a more stringent statistical treatment that resulted in a much reduced list of high confidence (at least 95%) proteins compared with their original findings. In order to facilitate the discovery of novel biomarkers in the future and to realize the...... full diagnostic potential of blood plasma, we feel that there is still a need for an ultra-high confidence reference list (at least 99% confidence) of blood plasma proteins. METHODS: To address the complexity and dynamic protein concentration range of the plasma proteome, we employed a linear ion...

  16. Mixed matrix membrane adsorbers for protein and blood purification

    OpenAIRE

    Saiful

    2007-01-01

    Biotechnology and bio-manufacturing markets are continuously growing, generating new sources of many valuable healthcare and life science products including therapeutic proteins and polysaccharides, monoclonals, vaccines, diagnostics, pharmaceutical chemicals and enzymes. These bioproducts have to be isolated and purified from complex mixtures before they can be used to ensure safety and efficacy. Nowadays, there are different techniques available for biomolecules isolation and purification. ...

  17. The relative nutritive value of irradiated spray-dried blood powder and heat-sterilized blood meal as measured in combination with whey protein

    International Nuclear Information System (INIS)

    A method of processing blood meal in which nutritive value of the protein is preserved is described, since appreciable losses occur in the nutritive value of the protein when prepared by heat sterilization with drying at atmospheric pressure in steam jacketed vessels. Blood was spray dried and irradiated at an intensity of 10 kGy. Collectively the heat of spray drying and irradiation was effective in killing both the virus plaque-forming units and the bacteria, thus producing a commercially acceptable sterile product of higher nutritive value. The relative nutritive values (RNV) of 50:50 protein were 0,56 for whey protein concentrate plus heat-sterilized blood meal and 0.90 for whey protein concentrate plus irradiated spray-dried blood powder. Whey protein concentrate used as a control has a RNV of 1,0

  18. Detection of Intracellular Factor VIII Protein in Peripheral Blood Mononuclear Cells by Flow Cytometry

    Directory of Open Access Journals (Sweden)

    Gouri Shankar Pandey

    2013-01-01

    Full Text Available Flow cytometry is widely used in cancer research for diagnosis, detection of minimal residual disease, as well as immune monitoring and profiling following immunotherapy. Detection of specific host proteins for diagnosis predominantly uses quantitative PCR and western blotting assays. In this study, we optimized a flow cytometry-based detection assay for Factor VIII protein in peripheral blood mononuclear cells (PBMCs. An indirect intracellular staining (ICS method was standardized using monoclonal antibodies to different domains of human Factor VIII protein. The FVIII protein expression level was estimated by calculating the mean and median fluorescence intensities (MFI values for each monoclonal antibody. ICS staining of transiently transfected cell lines supported the method's specificity. Intracellular FVIII protein expression was also detected by the monoclonal antibodies used in the study in PBMCs of five blood donors. In summary, our data suggest that intracellular FVIII detection in PBMCs of hemophilia A patients can be a rapid and reliable method to detect intracellular FVIII levels.

  19. Probing Bio-Nano Interactions between Blood Proteins and Monolayer-Stabilized Graphene Sheets.

    Science.gov (United States)

    Gan, Shiyu; Zhong, Lijie; Han, Dongxue; Niu, Li; Chi, Qijin

    2015-11-18

    Meeting proteins is regarded as the starting event for nanostructures to enter biological systems. Understanding their interactions is thus essential for a newly emerging field, nanomedicine. Chemically converted graphene (CCG) is a wonderful two-dimensional (2D) material for nanomedicine, but its stability in biological environments is limited. Systematic probing on the binding of proteins to CCG is currently lacking. Herein, we report a comprehensive study on the interactions between blood proteins and stabilized CCG (sCCG). CCG nanosheets are functionalized by monolayers of perylene leading to significant improvement in their resistance to electrolyte salts and long-term stability, but retain their core structural characteristics. Five types of model human blood proteins including human fibrinogen, γ-globulin, bovine serum albumin (BSA), insulin, and histone are tested. The main driving forces for blood protein binding involve the π-π interacations between the π-plane of sCCG and surface aromatic amonic acid (sAA) residues of proteins. Several key binding parameters including the binding amount, Hill coefficient, and binding constant are determined. Through a detailed analysis of key controlling factors, we conclude that the protein binding to sCCG is determined mainly by the protein size, the number, and the density of the sAA. PMID:26413807

  20. Aptamer-based surface plasmon resonance sensing of glycated human blood proteins

    Science.gov (United States)

    Reaver, Nathan G. F.; Zheng, Rui; Kim, Dong-Shik; Cameron, Brent D.

    2013-02-01

    The concentration ratio of glycated to non-glycated forms of various blood proteins can be used as a diagnostic measure in diabetes to determine a history of glycemic compliance. Depending on a protein's half-life in blood, compliance can be assessed from a few days to several months in the past, which can then be used to provide additional therapeutic guidance. Current glycated protein detection methods are limited in their ability to measure multiple proteins, and are susceptible to interference from other blood pathologies. In this study, we developed and characterized DNA aptamers for use in Surface Plasmon Resonance (SPR) sensors to assess the blood protein hemoglobin. The aptamers were developed by way of a modified Systematic Evolution of Ligands by Exponential Enrichment (SELEX) process which selects DNA sequences that have a high binding affinity to a specific protein. DNA products resulting from this process are sequenced and identified aptamers are then synthesized. The SELEX process was performed to produce aptamers for a glycated form of hemoglobin. Equilibrium dissociation constants for the binding of the identified aptamer to glycated hemoglobin, hemoglobin, and fibrinogen were calculated from fitted Langmuir isotherms obtained through SPR. These constants were determined to be 94 nM, 147 nM, and 244 nM respectively. This aptamer can potentially be used to create a SPR aptamer based biosensor for detection of glycated hemoglobin, a technology that has the potential to deliver low-cost and immediate glycemic compliance assessment in either a clinical or home setting.

  1. Adsorbed plasma proteins modulate the effects of single-walled carbon nanotubes on neutrophils in blood.

    Science.gov (United States)

    Vlasova, Irina I; Mikhalchik, Elena V; Barinov, Nikolay A; Kostevich, Valeria A; Smolina, Natalia V; Klinov, Dmitry V; Sokolov, Alexey V

    2016-08-01

    Proteins adsorbed on a surface may affect the interaction of this surface with cells. Here, we studied the binding of human serum albumin (HSA), fibrinogen (FBG) and immunoglobulin G (IgG) to PEGylated single-walled carbon nanotubes (PEG-SWCNTs) and evaluated the impact of PEG-SWCNT treated by these proteins on neutrophils in whole blood samples. Measurements of adsorption parameters revealed tight binding of proteins to PEG-SWCNTs. AFM was employed to directly observe protein binding to sidewalls of PEG-SWCNTs. Fluorescein-labeled IgG was used to ascertain the stability of PEG-SWCNT-IgG complexes in plasma. In blood samples, all plasma proteins mitigated damage of neutrophils observed just after blood exposure to PEG-SWCNTs, while only treatment of PEG-SWCNTs with IgG resulted in dose- and time-dependent enhancement of CNT-induced neutrophil activation and in potentiation of oxidative stress. Our study demonstrates the ability of adsorbed plasma proteins to influence neutrophil response caused by PEG-SWCNTs in whole blood. PMID:27015767

  2. Oxidation of Lipids and Proteins in Lens and Blood Plasma of Rats in Ageing

    Directory of Open Access Journals (Sweden)

    Ivanova I.P.

    2011-09-01

    Full Text Available The aim of the study is to assess the intensity of oxidation of lipids and proteins in lens and blood plasma of Wistar rats in ageing. Materials and Methods. The experiments were carried out on 25 Wistar male rats of four age groups: 5, 12, 24 and 36 months. Materials for study were lens and blood plasma. Lipids were extracted using Folch partition. The content of diene and triene conjugates was assessed by means of spectrophotometry. The level of Schiff’s bases was studied according to fluorescence intensity, malon dialdehyde concentration — according to the intensity of interaction with thiobarbituric acid. Potentiality of substrate oxidation in specimen was assessed using the method of induced chemoluminescence, and the degree of protein oxidative modification was assessed according to the level of carbonyl derivatives with 2.4-dinitrophenylhydrasine. The investigation of the content of total lipids and total proteins were carried out using “Bio-Test Total Lipids” and “Total Protein-Vital”. Results. The processes of lipid peroxidation of lens membranes are increasing in animals aged 5—12 months and decreasing in the period of 12—24 months. The level of lipid peroxidation in blood plasma has an expressed tendency for increasing in ageing. Over the years, there is the level decrease of carbonyl derivatives of aminoacids of lens proteins and the tendency for the increase of oxidative modification of proteins in blood plasma.

  3. Diminished Expression of Complement Regulatory Proteins on Peripheral Blood Cells from Systemic Lupus Erythematosus Patients

    OpenAIRE

    Ricardo Machado Xavier; João Carlos Tavares Brenol; Priscila Schmidt Lora; Odirlei Andre Monticielo; Amanda Kirchner Piccoli; Laiana Schneider; Ana Paula Alegretti

    2012-01-01

    CD55, CD59, CD46, and CD35 are proteins with complement regulatory (Creg) properties that ensure cell and tissue integrity when this system is activated. The aim of this study was to evaluate the Creg expression on peripheral blood cells from SLE patients and its association with cytopenia and disease activity. Flow cytometric analyses were performed on blood cells from 100 SLE patients and 61 healthy controls. Compared with healthy controls, we observed in SLE patients with lymphopenia and n...

  4. Untersuchungen zu invasionspezifischen Proteinen von Holospora obtusa, einem Bakterium aus dem Makronukleus von Paramecium caudatum

    OpenAIRE

    Schwarz, Steffen

    2002-01-01

    H.obtusa ist ein endonukleäres, Gram-negatives Bakterium, das ausschließlich den Makronukleus von Paramecium caudatum infiziert und dort lebt. Ziel war es, infektionsrelevante Proteine von Holospora obtusa zu finden und zu untersuchen. Es wurden Wechselwirkungen zwischen einem 51,5 kDa Oberflächenproteinen von H. obtusa und einem 67 kDa phagosomalen Protein von P. caudatum gefunden. Das Gen des 51,5 kDa Proteins wurde sequenziert und mit anderen Proteinen aus Datenbanken verglichen. Es zeigte...

  5. A new role for the von Hippel-Lindau tumor suppressor protein: stimulation of mitochondrial oxidative phosphorylation complex biogenesis

    Czech Academy of Sciences Publication Activity Database

    Hervouet, E.; Demont, J.; Pecina, Petr; Vojtíšková, Alena; Houštěk, Josef; Simonnet, H.; Godinot, C.

    2005-01-01

    Roč. 26, č. 3 (2005), s. 531-539. ISSN 0143-3334 R&D Projects: GA ČR GA303/03/0749; GA MZd NR7790 Institutional research plan: CEZ:AV0Z50110509 Keywords : von Hippel-Lindau * clear cell renal carcinoma (CCRC) * mitochondria Subject RIV: EB - Genetics ; Molecular Biology Impact factor: 5.108, year: 2005

  6. Electrophoretic pattern of blood serum proteins of some of the vertebrates of Pakistan

    International Nuclear Information System (INIS)

    The electrophoretic pattern of blood serum proteins of some of the common fishes e.g. Catla catla, Cirrhina mrigala, Channa punctatus, Channa marulius, Wallago attu, Heterop-neustes fossilis; amphibia e.g., Rana tigrina, Rana cyanophlyctis, Bufo melanostictus; reptiles e.g. Varanus bengalensis, Uromastix hardwickii; birds e.g. Columba livia, Gallus domesticus, Passer domestica, Anas platyrhynchos; and mammals e.g. Homo sapiens, Mus musculus, Lepus cuniculus have been described. The mobility of proteins of blood sera has been studied over cellulose acetate paper and then a comparative pattern analysed

  7. Effects of Electron Beam and Microwave Irradiation on Human Blood Proteins

    International Nuclear Information System (INIS)

    The effects of separated and combined accelerated electron beam (EB) of 6.23 MeV and microwave (MW) of 2.45GHz irradiation on proteins in samples of human serum, human plasma and human integral blood are presented. Also, it was studied the effect of separate and combined EB and MW irradiation on proteins irradiated in samples of human integral blood, without and in the presence of a synthetic compound solution (S.C.S.) which is expected to exhibit various biological actions, such as to diminish or to increase the irradiation effects

  8. Gold nanoparticles: role of size and surface chemistry on blood protein adsorption

    International Nuclear Information System (INIS)

    Material interaction with blood proteins is a critical issue, since it could influence the biological processes taking place in the body following implantation/injection. This is particularly important in the case of nanoparticles, where innovative properties, such as size and high surface to volume ratio can lead to a behavioral change with respect to bulk macroscopic materials and could be responsible for a potential risk for human health. The aim of this work was to compare gold nanoparticles (AuNP) and planar surfaces to study the role of surface curvature moving from the macro- to the nano-size in the process of blood protein adsorption. In the course of the study, different protocols were tested to optimize the analysis of protein adsorption on gold nanoparticles. AuNP with different size (10, 60 and 200 nm diameter) and surface coatings (citrate and polyethylene glycol) were carefully characterized. The stabilizing action of blood proteins adsorbed on AuNP was studied measuring the variation of size and solubility of the nanoparticles following incubation with single protein solutions (human serum albumin and fibrinogen) and whole blood plasma. In addition, we developed a method to elute proteins from AuNP to study the propensity of gold materials to adsorb plasma proteins in function of dimensional characteristics and surface chemistry. We showed a different efficacy of the various eluting media tested, proving that even the most aggressive agent cannot provide a complete detachment of the protein corona. Enhanced protein adsorption was evidenced on AuNP if compared to gold laminae (bare and PEGylated) used as macroscopic control, probably due to the superior AuNP surface reactivity.

  9. C-reactive protein prolongs blood coagulation time in phospholipids-dependent coagulation tests

    Directory of Open Access Journals (Sweden)

    L D Kozmin

    2003-01-01

    Full Text Available C-refctive protein prolongs blood coagulation time in phospholipids-dependent coagulation tests. O.P. Bliznukov, L.D. Kostin, A.J. Martinov, T.A. Lisitsina, T.M. Reshetnyak, V.J. Lauga Objective. To study influence of different CRP forms on blood clotting time in standard phospholipid clotting tests. Material and methods. Purified native CRP. monomeric CRP (0-1.6 M, immune complexes of native CRP and rabbit polyclonal anti-CRP antibodies (1.6 M were added to blood plasma of healthy donors. Blood clotting time was registered using optical coagulometer. Phospholipid dependent prothrombin time (PT, activated partial tromboplastin time (APTT, kaolin clotting time (KCT with kaolin and ellagic acid, dilute Russel viper venom time (dRVVT were determined. Results. Native CRP was able to increase blood clotting time in all mentioned clotting tests, excluding prothrombin time. CRP influence on blood clotting time showed a concentration dependence. Polyclonal rabbit anti-CRP antibodies had no inhibitory effect on CRP prolonged blood clotting time. Monomeric CRP (0-1.6 M had no influence on blood clotting time in all phospholipid-dependent clotting tests.

  10. Quantitative Proteomanalyse der Bindeproteine von Histon H3 und dem Four-and-a-Half-LIM 3 (FHL3) Protein

    OpenAIRE

    Klingberg, Rebecca

    2012-01-01

    The identification of specific protein-protein interactions is a basic requirement for a comprehensive understanding of many cellular processes. Mass spectrometric proteome analyses have uncovered a multitude of posttranslational protein modifications, such as protein phosphorylation, which regulate protein-protein interactions. These regulatory modifications add a new layer of complexity because they can either facilitate or block protein-protein interactions. The aim of this thesis was t...

  11. Inhibition of bacterial aggregation by serum- and blood-derived proteins.

    OpenAIRE

    Malamud, D; Brown, C; Goldman, R

    1984-01-01

    Human and animal sera contain potent inhibitors of saliva-mediated aggregation of oral streptococci. The inhibitors consist of a high-molecular-weight heat-labile factor and a lower-molecular-weight heat-activated factor. The latter appears to be serum albumin. Analyses of purified blood-derived proteins indicated that several high-molecular-weight proteins (fibrinogen, fibronectin, and ferritin) were able to inhibit aggregation at low concentrations. These data suggest that high-molecular-we...

  12. von Hippel-Lindau β-domain-luciferase fusion protein as a bioluminescent hydroxyproline sensor for a hypoxia-inducible factor prolyl hydroxylase assay.

    Science.gov (United States)

    Hong, Sungchae; Yum, Soohwan; Ha, Nam-Chul; Jung, Yunjin

    2010-12-15

    Hypoxia-inducible factor prolyl hydroxylases (HPHs) are responsible for hydroxylation of proline residues in hypoxia-inducible factor-α (HIF-α), resulting in von Hippel-Lindau (VHL)-mediated proteasome degradation of the hydroxylated proteins. Pharmacological inhibition of the enzyme leads to stabilization of HIF-α proteins and consequent activation of HIF, which provides therapeutic benefit for a variety of tissues undergoing ischemic stress. In an effort to develop a new assay for measuring HPH activity, we designed a fusion protein, VHL β-domain-luciferase. Recombinant fusion protein with a glutathione S-transferase (GST) tag was purified from Escherichia coli. GST-VHL β-domain-luciferase with C-terminal deletion (GVbL-CD) was obtained as a major product and found to have luciferase activity. In a GVbL-CD capture assay using HIF peptide-bound beads, at least a 13-fold increase in luciferase activity was elicited for HIF peptide with hydroxyproline compared with unhydroxylated HIF peptide. HPH inhibitory activities of known HPH inhibitors or HIF-1α inducers were assessed using this assay, whose results were in good agreement with those obtained from conventional methods. The competitive effect of 2-ketoglutarate on dimethyloxalylglycine-mediated HPH inhibition was assessed very well in the new assay. Taken together, the VHL β-domain protein with luciferase activity is of use for HPH activity assay. PMID:20705044

  13. Blood parameters in growing pigs fed increasing levels of bacterial protein meal

    DEFF Research Database (Denmark)

    Hellwing, Anne Louise Frydendahl; Tauson, Anne-Helene; Skrede, Anders

    2007-01-01

    The experiment investigated the effects of increasing dietary levels of bacterial protein meal (BPM) on various blood parameters reflecting protein and fat metabolism, liver function, and purine base metabolism in growing pigs. Sixteen barrows were allocated to four different experimental diets....... The control diet was based on soybean meal. In the other three diets soybean meal was replaced with increasing levels of BPM, approximately 17%, 35%, and 50% of the nitrogen being derived from BPM. Blood samples from the jugular vein were taken when the body weights of the pigs were approximately 10 kg, 21 kg......, 45 kg, and 77 kg. The blood parameters reflecting fat metabolism and liver funtion were not affected by diet. Both the plasma albumin and uric acid concentrations tended to decrease (P = 0.07 and 0.01, respectively) with increasing dietary BPM content, whereas the plasma glucose concentration tended...

  14. Reduced Cytotoxicity of Graphene Nanosheets Mediated by Blood-Protein Coating.

    Science.gov (United States)

    Chong, Yu; Ge, Cuicui; Yang, Zaixing; Garate, Jose Antonio; Gu, Zonglin; Weber, Jeffrey K; Liu, Jiajia; Zhou, Ruhong

    2015-06-23

    The advent and pending wide use of nanoscale materials urges a biosafety assessment and safe design of nanomaterials that demonstrate applicability to human medicine. In biological microenvironment, biomolecules will bind onto nanoparticles forming corona and endow nanoparticles new biological identity. Since blood-circulatory system will most likely be the first interaction organ exposed to these nanomaterials, a deep understanding of the basic interaction mechanisms between serum proteins and foreign nanoparticles may help to better clarify the potential risks of nanomaterials and provide guidance on safe design of nanomaterials. In this study, the adsorption of four high-abundance blood proteins onto the carbon-based nanomaterial graphene oxide (GO) and reduced GO (rGO) were investigated via experimental (AFM, florescence spectroscopy, SPR) and simulation-based (molecular dynamics) approaches. Among the proteins in question, we observe competitive binding to the GO surface that features a mélange of distinct packing modes. Our MD simulations reveal that the protein adsorption is mainly enthalpically driven through strong π-π stacking interactions between GO and aromatic protein residues, in addition to hydrophobic interactions. Overall, these results were in line with previous findings related to adsorption of serum proteins onto single-walled carbon nanotubes (SWCNTs), but GO exhibits a dramatic enhancement of adsorption capacity compared to this one-dimensional carbon form. Encouragingly, protein-coated GO resulted in a markedly less cytotoxicity than pristine and protein-coated SWCNTs, suggesting a useful role for this planar nanomaterial in biomedical applications. PMID:26040772

  15. Identification of Residual Blood Proteins in Ticks by Mass Spectrometry Proteomics

    OpenAIRE

    Wickramasekara, Samanthi; Bunikis, Jonas; Wysocki, Vicki; Barbour, Alan G.

    2008-01-01

    Mass spectrometry–based proteomics of individual ticks demonstrated persistence of mammalian host blood components, including α- and β-globin chains, histones, and mitochondrial enzymes, in Ixodes scapularis and Amblyomma americanum ticks for months after molting. Residual host proteins may identify sources of infection for ticks.

  16. Proteolytic system of blood-feeding ticks: An update on protein structures

    Czech Academy of Sciences Publication Activity Database

    Kovářová, Zuzana; Hobizalová, Radka; Žebrakovská, Iva; Brynda, Jiří; Řezáčová, Pavlína; Horn, Martin; Mareš, Michael

    2015-01-01

    Roč. 22, č. 1 (2015), s. 43-44. ISSN 1211-5894. [Discussions in Structural Molecular Biology. Annual Meeting of the Czech Society for Structural Biology /13./. 19.03.2015-21.03.2015, Nové Hrady] Institutional support: RVO:61388963 ; RVO:68378050 Keywords : blood-feeding ticks * protein structure * proteolytic system Subject RIV: CE - Biochemistry

  17. Central Gi(2) proteins, sympathetic nervous system and blood pressure regulation

    Czech Academy of Sciences Publication Activity Database

    Zicha, Josef

    2016-01-01

    Roč. 216, č. 3 (2016), s. 258-259. ISSN 1748-1708 Institutional support: RVO:67985823 Keywords : inhibitory G proteins * sympathetic nervous system * central blood pressure control Subject RIV: FA - Cardiovascular Diseases incl. Cardiotharic Surgery Impact factor: 4.382, year: 2014

  18. The plasma protein fibrinogen stabilizes clusters of red blood cells in microcapillary flows

    CERN Document Server

    Brust, M; Thiebaud, M; Flormann, D; Verdier, C; Kaestner, L; Laschke, M W; Selmi, H; Benyoussef, A; Podgorski, T; Coupier, G; Misbah, C; Wagner, C

    2014-01-01

    The supply of oxygen and nutrients and the disposal of metabolic waste in the organs depend strongly on how blood, especially red blood cells, flow through the microvascular network. Macromolecular plasma proteins such as fibrinogen cause red blood cells to form large aggregates, called rouleaux, which are usually assumed to be disaggregated in the circulation due to the shear forces present in bulk flow. This leads to the assumption that rouleaux formation is only relevant in the venule network and in arterioles at low shear rates or stasis. Thanks to an excellent agreement between combined experimental and numerical approaches, we show that despite the large shear rates present in microcapillaries, the presence of either fibrinogen or the synthetic polymer dextran leads to an enhanced formation of robust clusters of red blood cells, even at haematocrits as low as 1%. Robust aggregates are shown to exist in microcapillaries even for fibrinogen concentrations within the healthy physiological range. These pers...

  19. Probing Bio-Nano Interactions between Blood Proteins and Monolayer-Stabilized Graphene Sheets

    DEFF Research Database (Denmark)

    Gan, Shiyu; Zhong, Lijie; Han, Dongxue;

    2015-01-01

    Meeting proteins is regarded as the starting event for nanostructures to enter biological systems. Understanding their interactions is thus essential for a newly emerging field, nanomedicine. Chemically converted graphene (CCG) is a wonderful two-dimesional (2D) material for nanomedecine, but its...... stability in biological environments is limited. Systematic probing on the binding of proteins to CCG is currently lacking. Herein, we report a comprehensive study on the interactions between blood proteins and stabilized CCG (sCCG). CCG nanosheets are functionalized by monolayers of perylene leading to...

  20. Plasma protein thiols, ceruloplasmin, C-reactive protein and red blood cell acetylcholinesterase in patients undergoing intrauterine insemination

    OpenAIRE

    Krishnananda Prabhu; Pratap Kumar; Satish Kumar Adiga; Anjali Rao; Anupama Lanka; Jaipal Singh

    2009-01-01

    Objective: To estimate acetylcholinesterase (AChE), protein thiols (PT), ceruloplasmin (CP) and C-reactive proteins (CRPs) to assess any change in their levels following intrauterine insemination (IUI). Materials and Methods: Forty-two patients aged 31 ± 4.65 years (mean ± SD) with primary infertility selected for IUI. All of them had induced ovulation with clomiphene citrate 50 mg from day 2 to day 6. After taking the consent, 2 ml of blood was withdrawn before and after 24 h of IUI for bio...

  1. Identification of transferrin as the principal neptunium-binding protein in the blood serum of rats

    Energy Technology Data Exchange (ETDEWEB)

    Wirth, R.; Taylor, D.M.; Duffield, J.

    1985-01-01

    The binding of 239Np(V) to blood serum components of rats was examined in vivo and in vitro. After gel filtration of the serum using a Sephacryl S-300 column, 98% of the applied activity appeared with protein fractions representing coeluted albumins and transferrin. A separation of the albumin- and transferrin-proteins by ion-exchange chromatography using DEAE-cellulose showed the 239Np being entirely bound to the iron-carrier protein transferrin. The high elution yields from the ion-exchange columns, greater than 90%, suggest that the binding may be quite strong. The binding capacity of transferrin for neptunium in vivo was found to decline when the iron level in blood serum was increased. Precipitation experiments showed that 84 +/- 2% of the 239Np was precipitated with 10% (w/v) trichloracetic acid, 77 +/- 3% with 90% ethanol but only 6 +/- 1% with saturated ammonium sulphate at pH 7.4. The available data indicate that as for plutonium, thorium, americium and curium, the iron transport protein, transferrin, may be the main carrier protein for neptunium in mammalian blood serum.

  2. Effects of Different Exercise Intensities with Isoenergetic Expenditures on C-Reactive Protein and Blood Lipid Levels

    Science.gov (United States)

    Tsao, Te Hung; Yang, Chang Bin; Hsu, Chin Hsing

    2012-01-01

    We investigated the effects of different exercise intensities on C-reactive protein (CRP), and whether changes in CRP levels correlated with blood lipid levels. Ten men exercised at 25%, 65%, and 85% of their maximum oxygen consumption rates. Participants' blood was analyzed for CRP and blood lipid levels before and after the exercise sessions.…

  3. Activation of blood clotting and fibrinolysis in angiocardiography with ionic and non-ionic contrast medium; Aktivierung von Blutgerinnung und Fibrinolyse nach Angiokardiographie mit ionischem und nichtionischem Kontrastmittel

    Energy Technology Data Exchange (ETDEWEB)

    Winkler, U.H. [Gerinnungsphysiologisches Lab., Zentrum fuer Frauenheilkunde, Universitaetsklinikum Essen (Germany); Park, J.W. [Herz-Zentrum Kaiser-Wilhelm-Krankenhaus, Duisburg (Germany); Weber, S. [Schering AG, Geschaeftsbereich Deutschland, Berlin (Germany); Kothe, A. [Gerinnungsphysiologisches Lab., Zentrum fuer Frauenheilkunde, Universitaetsklinikum Essen (Germany); Schnitker, J. [Inst. fuer Angewandte Statistik GmbH, Bielefeld (Germany); Behrends-Steins, B. [Schering AG, Geschaeftsbereich Deutschland, Berlin (Germany); Albring, M. [Schering AG, Geschaeftsbereich Deutschland, Berlin (Germany)

    1997-06-01

    Purpose: To study the effects of a ionic (amidotrizoate) and a nonionic X-ray contrast medium (iopromid) during routine levocardiography and coronary angiography, we employed assays that detect reaction products of thrombin and plasmin to assess the activation of the haemostatic system. Methods: Subsequent to informed consent, 20 patients were randomly assigned to receive either amidotrizoate or iopromid during standard levocardiography and coronary angiography in a double-blind comparative study. Groups were comparable in respect of age, weight, sex and severity of the disease. No anticoagulation was provided. Coronary angiography was performed according to a standardised protocol. Consumption of contrast media and duration of the examination were comparable in both groups. Results: Thrombin generation (F.1+2) and thrombin activity (TAT) were higher with the ionic contrast medium but did not attain statistical significance. Fibrin generation and degradation as expressed by D-dimer fibrin split products was significantly increased in patients who had been receiving amidotrizoate (p<0,05, U-Test). Conclusion: The non-ionic X-ray contrast medium induced significantly less haemostatic activation in vivo than did the ionic medium amidotrizoate. These data suggest that earlier in vitro observations of more pronounced anticoagulant effects of ionic X-ray contrast media are of limited significance for the evaluation of in vivo effects of X-ray contrast media on haemostatic function. (orig.) [Deutsch] Ziel: Bestimmung der Aktivitaet des haemostatischen Systems in vivo nach Gabe von ionischem und nichtionischem Roentgenkontrastmittel in der Angiokardiographie. Material und Methoden: In eine randomisierte doppelblinde Vergleichsstudie mit den Kontrastmitteln Amidotrizoat und Iopromid wurden 20 Patienten (10 pro Gruppe) einbezogen. Bei der aus medizinischen Gruenden indizierten Angiokardiographie wurden 5 repraesentative Reaktionsprodukte der thrombin- und plasmininduzierten

  4. [The effect of blood serum proteins from the seal on the analgetic action of narcotic analgesics].

    Science.gov (United States)

    Aslaniants, Zh K; Melik-Eganov, G R; Evstratov, A V; Ivanov, M P; Batrakov, S G; Korobov, N V; Iasnetsov, V V

    1991-11-01

    The protein fraction isolated from blood of seal, Phoca groenlandica, has been found to produce hyperalgesic effect on rats exposed to thermic or electrocutaneous nociceptive stimulation, but fail to affect writhes provoked by intraperitoneal injection of acetic acid solution on mice. When combined with morphine, the fraction lowered completely its narcotic analgetic action in the above mentioned tests. On the contrary, these same proteins combined with promedol or fentanil enhanced and prolonged analgetic effect of the latter. Tested in vitro the protein showed neither opioid nor anti-opioid activity. Therefore it is reasonable to suppose that neurophysiological activity of the isolated fraction is due to the peptides formed on enzymatic hydrolysis of proteins in vivo rather than these proteins as such. PMID:1687360

  5. The plasma protein fibrinogen stabilizes clusters of red blood cells in microcapillary flows

    Science.gov (United States)

    Brust, M.; Aouane, O.; Thiébaud, M.; Flormann, D.; Verdier, C.; Kaestner, L.; Laschke, M. W.; Selmi, H.; Benyoussef, A.; Podgorski, T.; Coupier, G.; Misbah, C.; Wagner, C.

    2014-03-01

    The supply of oxygen and nutrients and the disposal of metabolic waste in the organs depend strongly on how blood, especially red blood cells, flow through the microvascular network. Macromolecular plasma proteins such as fibrinogen cause red blood cells to form large aggregates, called rouleaux, which are usually assumed to be disaggregated in the circulation due to the shear forces present in bulk flow. This leads to the assumption that rouleaux formation is only relevant in the venule network and in arterioles at low shear rates or stasis. Thanks to an excellent agreement between combined experimental and numerical approaches, we show that despite the large shear rates present in microcapillaries, the presence of either fibrinogen or the synthetic polymer dextran leads to an enhanced formation of robust clusters of red blood cells, even at haematocrits as low as 1%. Robust aggregates are shown to exist in microcapillaries even for fibrinogen concentrations within the healthy physiological range. These persistent aggregates should strongly affect cell distribution and blood perfusion in the microvasculature, with putative implications for blood disorders even within apparently asymptomatic subjects.

  6. Nifedipine effect on the labelling of blood cells and plasma proteins with Tc-99m

    International Nuclear Information System (INIS)

    The labeling of red blood cells (RBC) with Tc-99m depends on the presence of stannous ion (Sn) that helps this radionuclide's fixation on the hemoglobin molecule. Nifedipine is an agent capable to block a specific way where calcius (Ca) ion acrosses the cellular membrane and to bind itself on plasma proteins. The effect of nifedipine in the labeling of RBC and plasma proteins with Tc-99m was studied because of similarities between Ca and Sn ions. Blood with anticoagulant was treated with nifedipine concentration of 10-6M for 15 min at 370C. The labeling of RBC with Tc-99m was done incubating with Sn ion solution (3 uM) for different times. The % of radioactivity in RBC was determined. Samples of plasma were precipited with trichloroacetic acid and the % of radiocctivity in insoluble fraction was calculated. The same procedure was done using different nifedipine concentrations and the blood was incubated for 60 min with Sn ion. The determination of the % of Tc-99m labeled in RBC and plasma proteins showed that this drug does not have the capability to alter this incorporation because the results are similar to control. It is suggested that the Sn ions passage across RBC is not altered by nifedipine although this drug could bind to plasma protein, it does not modify the Tc-99m fixation on it. (author)

  7. Optimierung und Evaluierung von Enzymimmuntests zum Nachweis von Cephalosporin-Antibiotika in Milch

    OpenAIRE

    Bremus, Anna Caroline

    2012-01-01

    Die vorliegende Arbeit befasst sich mit der Entwicklung von monoklonalen Antikörpern (mAk) zum Nachweis verschiedener relevanter Cephalosporin-Rückstände. Zu diesem Zweck wurden einfache, schnelle und effiziente Verfahren zur Synthese von Cephalosporin-Protein-Konjugaten entwickelt. Zur Gewinnung von Antikörpern wurden Mäuse mit den entsprechenden, mittels aktiver-Ester-Methode an Trägerproteine gekoppelten Cephalosporinen immunisiert. Mittels Zellfusion konnten verschiedene ...

  8. Blood pressure lowering effect of a pea protein hydrolysate in hypertensive rats and humans.

    Science.gov (United States)

    Li, Huan; Prairie, Natalie; Udenigwe, Chibuike C; Adebiyi, Abayomi P; Tappia, Paramjit S; Aukema, Harold M; Jones, Peter J H; Aluko, Rotimi E

    2011-09-28

    The blood pressure lowering effect of a pea protein hydrolysate (PPH) that contained isolated by membrane ultrafiltration from the thermolysin digest of pea protein isolate (PPI), was examined using different rat models of hypertension as well as hypertensive human subjects. The PPH showed weak in vitro activities against renin and angiotensin converting enzyme (ACE) with inhibitory activities of 17 and 19%, respectively, at 1 mg/mL test concentration. Oral administration of the PPH to spontaneously hypertensive rats (SHR) at doses of 100 and 200 mg/kg body weight led to a lowering of hourly systolic blood pressure (SBP), with a maximum reduction of 19 mmHg at 4 h. In contrast, orally administered unhydrolyzed PPI had no blood pressure reducing effect in SHR, suggesting that thermolysin hydrolysis may have been responsible for releasing bioactive peptides from the native protein. Oral administration of the PPH to the Han:SPRD-cy rat (a model of chronic kidney disease) over an 8-week period led to 29 and 25 mmHg reductions in SBP and diastolic blood pressure, respectively. The PPH-fed rats had lower plasma levels of angiotensin II, the major vasopressor involved in development of hypertension, but there was no effect on plasma activity or renal mRNA levels of ACE. However, renal expression of renin mRNA levels was reduced by approximately 50% in the PPH-fed rats, suggesting that reduced renin may be responsible for the reduced levels of angiotensin II. In a 3-week randomized double blind placebo-controlled crossover human intervention trial (7 volunteers), significant (p<0.05) reductions (over placebo) in SBP of 5 and 6 mmHg were obtained in the second and third weeks, respectively, for the PPH group. Therefore, thermolysin derived bioactive peptides from PPH reduced blood pressure in hypertensive rats and human subjects, likely via effects on the renal angiotensin system. PMID:21854068

  9. An essential malaria protein defines the architecture of blood-stage and transmission-stage parasites.

    Science.gov (United States)

    Absalon, Sabrina; Robbins, Jonathan A; Dvorin, Jeffrey D

    2016-01-01

    Blood-stage replication of the human malaria parasite Plasmodium falciparum occurs via schizogony, wherein daughter parasites are formed by a specialized cytokinesis known as segmentation. Here we identify a parasite protein, which we name P. falciparum Merozoite Organizing Protein (PfMOP), as essential for cytokinesis of blood-stage parasites. We show that, following PfMOP knockdown, parasites undergo incomplete segmentation resulting in a residual agglomerate of partially divided cells. While organelles develop normally, the structural scaffold of daughter parasites, the inner membrane complex (IMC), fails to form in this agglomerate causing flawed segmentation. In PfMOP-deficient gametocytes, the IMC formation defect causes maturation arrest with aberrant morphology and death. Our results provide insight into the mechanisms of replication and maturation of malaria parasites. PMID:27121004

  10. An essential malaria protein defines the architecture of blood-stage and transmission-stage parasites

    Science.gov (United States)

    Absalon, Sabrina; Robbins, Jonathan A.; Dvorin, Jeffrey D.

    2016-01-01

    Blood-stage replication of the human malaria parasite Plasmodium falciparum occurs via schizogony, wherein daughter parasites are formed by a specialized cytokinesis known as segmentation. Here we identify a parasite protein, which we name P. falciparum Merozoite Organizing Protein (PfMOP), as essential for cytokinesis of blood-stage parasites. We show that, following PfMOP knockdown, parasites undergo incomplete segmentation resulting in a residual agglomerate of partially divided cells. While organelles develop normally, the structural scaffold of daughter parasites, the inner membrane complex (IMC), fails to form in this agglomerate causing flawed segmentation. In PfMOP-deficient gametocytes, the IMC formation defect causes maturation arrest with aberrant morphology and death. Our results provide insight into the mechanisms of replication and maturation of malaria parasites. PMID:27121004

  11. Further insight into the roles of the glycans attached to human blood protein C inhibitor

    DEFF Research Database (Denmark)

    Sun, Wei; Parry, Simon; Ubhayasekera, Wimal;

    2010-01-01

    . Furthermore, we have provided experimental evidence that PCI in both individuals is O-glycosylated on Thr20 with a core type 1 O-glycan, which is mostly NeuAcGalGalNAc. Modeling suggested that the O-glycan attachment site is located in proximity to several ligand-binding sites of the inhibitor.......Protein C inhibitor (PCI) is a 57-kDa glycoprotein that exists in many tissues and secretions in human. As a member of the serpin superfamily of proteins it displays unusually broad protease specificity. PCI is implicated in the regulation of a wide range of processes, including blood coagulation...

  12. Optical tweezers study of red blood cell aggregation and disaggregation in plasma and protein solutions

    Science.gov (United States)

    Lee, Kisung; Kinnunen, Matti; Khokhlova, Maria D.; Lyubin, Evgeny V.; Priezzhev, Alexander V.; Meglinski, Igor; Fedyanin, Andrey A.

    2016-03-01

    Kinetics of optical tweezers (OT)-induced spontaneous aggregation and disaggregation of red blood cells (RBCs) were studied at the level of cell doublets to assess RBC interaction mechanics. Measurements were performed under in vitro conditions in plasma and fibrinogen and fibrinogen + albumin solutions. The RBC spontaneous aggregation kinetics was found to exhibit different behavior depending on the cell environment. In contrast, the RBC disaggregation kinetics was similar in all solutions qualitatively and quantitatively, demonstrating a significant contribution of the studied proteins to the process. The impact of the study on assessing RBC interaction mechanics and the protein contribution to the reversible RBC aggregation process is discussed.

  13. Blood parameters in growing pigs fed increasing levels of bacterial protein meal

    Directory of Open Access Journals (Sweden)

    Tauson Anne-Helene

    2007-11-01

    Full Text Available Abstract The experiment investigated the effects of increasing dietary levels of bacterial protein meal (BPM on various blood parameters reflecting protein and fat metabolism, liver function, and purine base metabolism in growing pigs. Sixteen barrows were allocated to four different experimental diets. The control diet was based on soybean meal. In the other three diets soybean meal was replaced with increasing levels of BPM, approximately 17%, 35%, and 50% of the nitrogen being derived from BPM. Blood samples from the jugular vein were taken when the body weights of the pigs were approximately 10 kg, 21 kg, 45 kg, and 77 kg. The blood parameters reflecting fat metabolism and liver function were not affected by diet. Both the plasma albumin and uric acid concentrations tended to decrease (P = 0.07 and 0.01, respectively with increasing dietary BPM content, whereas the plasma glucose concentration tended to increase (P = 0.07 with increasing dietary BPM content. It was concluded that up to 50% of the nitrogen could be derived from BPM without affecting metabolic function, as reflected in the measured blood parameters.

  14. Immunization of Aotus monkeys with Plasmodium falciparum blood-stage recombinant proteins.

    OpenAIRE

    S Herrera; Herrera, M. A.; Perlaza, B L; Burki, Y; Caspers, P; Döbeli, H; Rotmann, D; Certa, U

    1990-01-01

    The current spread of multidrug-resistant malaria demands rapid vaccine development against the major pathogen Plasmodium falciparum. The high quantities of protein required for a worldwide vaccination campaign select recombinant DNA technology as a practical approach for large-scale antigen production. We describe the vaccination of Aotus monkeys with two recombinant blood-stage antigens (recombinant p41 and 190N) that were considered as vaccine candidates because parasite-derived antigen pr...

  15. Blood protein turnover in parasitized ruminants. The influence of host nutrition

    International Nuclear Information System (INIS)

    Ruminants infected with helminth or protozoal parasites generally become anaemic and hypoalbuminaemic, as well as losing their appetite. Since feed intake plays an important part in determining blood protein levels, it is necessary, when attempting to determine the mechanisms by which parasites cause anaemia and hypoalbuminaemia, to differentiate between the effects of feed intake per se and the specific effects of the parasite on blood protein turnover. This can be done by a variety of radioisotope techniques using infected and pair-fed control animals. Additionally, animals on a poor plane of nutrition suffer more from parasitism than those which are well fed. To understand the reason for this, it is necessary to determine whether diet influences susceptibility to parasite establishment or survival, and/or susceptibility to the metabolic consequences of parasitism. Described here is the current state of knowledge on the interaction between host nutrition and susceptibility to parasitic infection and parasitic disease processes, with particular reference to anaemia and hypoalbuminaemia. It is concluded that there is little evidence that nutrition has a significant bearing on resistance or susceptibility to infection, but that it does not have a profound influence on the ability of animals to withstand the pathogenic effects of parasites. The reasons for this are discussed in detail, but the principal benefit of a good plane of nutrition is that it enables the synthetic machinery of the host to keep pace with the concurrent parasite-induced hypercatabolism of blood proteins. (author)

  16. Effects of a protein glycocalyx in the hemodynamics of small blood vessels

    Science.gov (United States)

    Dimakopoulos, Yiannis; Delidakis, George; Tsamopoulos, John

    2015-11-01

    Glycocalyx is a protein layer of approximate thickness 0.5 μm that lines vessel walls. We study the effects this layer has on the blood flow inside arterioles and venules, where the relative size of the glycocalyx is significant. To properly describe phenomena that naturally occur in blood flow, such as the inhomogeneous distribution of red blood cells and their aggregation, we use an improved viscoelastic constitutive model. The glycocalyx layer is modeled as fixed porous media. Cells cannot penetrate inside it, since its hydraulic permeability is very low, and the flow inside this layer is described by the equations for a viscous fluid with an extra Brinkman term to account for the effects the porous medium has on the flow. The closed set of equations is solved using the Finite Element method, assuming steady-state with dependence only in the r-direction. Our results are favorably compared with the in vivo velocity profiles in venules of mice produced by Damiano et al. (2004) and the formation of cell-free layer near glycocalyx. Flow inside the glycocalyx layer is found to be severely attenuated due to the low hydraulic permeability, which can have interesting implications in the transport of various substances form the blood to the tissues or in the use of shear stresses as signals for the endothelial surface cells. Finally, we simulate the transient blood flow under pulsatile conditions.

  17. [MORPHOFUNCTIONAL STATE OF BLOOD CELLS AFTER CHRONIC EXPOSURE OF THE PROTEIN KINASES INHIBITOR MALEIMIDE DERIVATIVE].

    Science.gov (United States)

    Byelinska, I V; Lynchak, O V; Tsyvinska, S M; Rybalchenko, V K

    2015-01-01

    The effect of the protein kinases inhibitor maleimide derivative (MI-1, 1-(4-Cl-benzyl)-3-Cl-4-(CF3-phenylamino)-1H-pyrrole-2,5-dione), inhibitor of VEGF-R1,2,3, FGF-R1, EGF-R(h), PDK1, Src(h), Syk(h), YES, ZAP70 et al. with antineoplastic activity, on blood cells parameters of rats after chronic exposure has been studied. Administration of MI-1 at doses 0.027 and 2.7 mg/kg (suppress colon carcinogenesis) for 20 and 26 weeks does not affect the morphofunctional state of red blood cells in healthy rats. This is confirmed by the lack of differences in the concentration of hemoglobin in blood, red blood cells count, mean corpuscular hemoglobin and mean corpuscular hemoglobin concentration, hematocrit and mean corpuscular volume, and the number of reticulocytes in blood after 20 and 26 weeks of exposure compared with the control group. MI-1 at indicated doses does not influence total leukocytes count and content (eosinophilic and neutrophilic granulocytes, lymphocytes, monocytes) and does not inhibit thrombocytopoiesis (platelet count remains unchanged). No negative effect of MI-1 on hematopoiesis is not limited (by the hemopoietic system) use of this compound as a potential antitumor drug PMID:26552308

  18. Analysis of zinc oxide nanoparticles binding proteins in rat blood and brain homogenate

    Directory of Open Access Journals (Sweden)

    Shim KH

    2014-12-01

    Full Text Available Kyu Hwan Shim,1 John Hulme,1 Eun Ho Maeng,2 Meyoung-Kon Kim,3 Seong Soo A An1 1Department of Bionano Technology, Gachon Medical Research Institute, Gachon University, Sungnam-si, Gyeonggi-do, South Korea; 2Department of Analysis, KTR, Kimpo, Gyeonggi-do, South Korea; 3Department of Biochemistry and Molecular Biology, Korea University Medical School and College, Seoul, South Korea Abstract: Nanoparticles (NPs are currently used in chemical, cosmetic, pharmaceutical, and electronic products. Nevertheless, limited safety information is available for many NPs, especially in terms of their interactions with various binding proteins, leading to potential toxic effects. Zinc oxide (ZnO NPs are included in the formulation of new products, such as adhesives, batteries, ceramics, cosmetics, cement, glass, ointments, paints, pigments, and supplementary foods, resulting in increased human exposures to ZnO. Hence, we investigated the potential ZnO nanotoxic pathways by analyzing the adsorbed proteins, called protein corona, from blood and brain from four ZnO NPs, ZnOSM20(-, ZnOSM20(+, ZnOAE100(-, and ZnOAE100(+, in order to understand their potential mechanisms in vivo. Through this study, liquid chromatography–mass spectroscopy/mass spectroscopy technology was employed to identify all bound proteins. Totals of 52 and 58 plasma proteins were identified as being bound to ZnOSM20(- and ZnOSM20(+, respectively. For ZnOAE100(- and ZnOAE100(+, 58 and 44 proteins were bound, respectively. Similar numbers of proteins were adsorbed onto ZnO irrespective of size or surface charge of the nanoparticle. These proteins were further analyzed with ClueGO, a Cytoscape plugin, which provided gene ontology and the biological interaction processes of identified proteins. Interactions between diverse proteins and ZnO nanoparticles could result in an alteration of their functions, conformation, and clearance, eventually affecting many biological processes. Keywords: brain

  19. von Neumann Morgenstern Preferences

    DEFF Research Database (Denmark)

    Vind, Karl

    von Neumann Morgenstern utility is generalized to von Neumann Morgenstern preferences. The proof is an application of simple hyperplane theorems......von Neumann Morgenstern utility is generalized to von Neumann Morgenstern preferences. The proof is an application of simple hyperplane theorems...

  20. von Neumann Morgenstern Preferences

    DEFF Research Database (Denmark)

    Vind, Karl

    2000-01-01

    von Neumann Morgenstern utility is generalized to von Neumann Morgenstern preferences. The proof is an application of simple hyperplane theorems......von Neumann Morgenstern utility is generalized to von Neumann Morgenstern preferences. The proof is an application of simple hyperplane theorems...

  1. Clinical significance of determination of cord blood S100B protein contents in neonates with intrauterine asphyxia

    International Nuclear Information System (INIS)

    Objective: To study the clinical significance of determination of cord blood S100B protein (a specific neural glia protein reflecting CNS injury) in neonates with intrauterine asphyxia. Methods: Cord blood S100B protein contents were measured with ELISA in (1) 41 neonates from normal vaginal delivery (2) 48 neonates from caesarean section without asphyxia and (3) 36 caesarean section neonates with intrauterine asphyxia. Results: Cord blood S100B protein contents in neonates with intrauterine asphyxia were significantly higher than those in the other 2 groups (P0.05). Conclusion: Cord blood S100B protein is a uesful indicator for the diagnosis of neonatal asphyxia and identification of brain damage following neonatal asphyxia. (authors)

  2. Proteins involved in invasion of human red blood cells by malaria parasites

    Directory of Open Access Journals (Sweden)

    Ewa Jaśkiewicz

    2010-11-01

    Full Text Available Malaria is a disease caused by parasites of Plasmodium species. It is responsible for around 1-2 million deaths annually, mainly children under the age of 5. It occurs mainly in tropical and subtropical areas.Malaria is caused by five Plasmodium species:[i] P. falciparum, P. malariae, P. vivax, P. knowlesi[/i] and [i]P. ovale[/i]. Mosquitoes spread the disease by biting humans. The malaria parasite has two stages of development: the human stage and the mosquito stage. The first stage occurs in the human body and is divided into two phases: the liver phase and the blood phase.The invasion of erythrocytes by [i]Plasmodium[/i] merozoites is a multistep process of specific protein interactions between the parasite and red blood cell. The first step is the reversible merozoite attachment to the erythrocyte followed by its apical reorientation, then formation of an irreversible “tight” junction and finally entry into the red cell in a parasitophorous vacuole.The blood phase is supported by a number of proteins produced by the parasite. The merozoite surface GPI-anchored proteins (MSP-1, 2, 4, 5, 8 and 10 assist in the process of recognition of susceptible erythrocytes, apical membrane antigen (AMA-1 may be directly responsible for apical reorientation of the merozoite and apical proteins which function in tight junction formation. These ligands are members of two families: Duffy binding-like (DBL and reticulocyte binding-like (RBL proteins. In [i]Plasmodium[/i] [i]falciparum[/i] the DBL family includes: EBA-175, EBA-140 (BAEBL, EBA-181 (JESEBL, EBA-165 (PEBL and EBL-1 ligands.To date, no effective antimalarial vaccine has been developed, but there are several studies for this purpose. Therefore, it is crucial to understand the molecular basis of host cells invasion by parasites. Major efforts are focused on developing a multiantigenic and multiepitope vaccine preventing all steps of [i]Plasmodium[/i] invasion.

  3. INFLUENCE OF NATURAL IMMUNOMODULATORS ON PROTEIN FRACTIONS AND CORTISOL CONTENT IN RABBIT BLOOD UNDER STRESS

    Directory of Open Access Journals (Sweden)

    Grabovskyi S.

    2015-08-01

    Full Text Available The results of determination of protein fractions, cortisol content in blood of rabbits, which further added to the feed of natural origin biologically active substances are presented in the article. As an antistressors and immunomodulators in pre-slaughter period are using of spleen extract biologically active substances were obtained with ultrasound application. The purpose of research — determination of changes of protein fractions, cortisol content in rabbits blood before slaughter and their correction of natural origin biologically active substances (spleen extract. Object and research methods. The experiment was conducted on 15 rabbits with standard diet. Three groups of rabbits five month of age (5 rabbits each was formed for research. The spleen extract were using as an biologically active substances to the feed rabbits in pre-slaughter period (five days before slaughter. The extracts were applied to feed by aerosol method (70 °alcohol solution of spleen extract volume of 1.4 ml per rabbit (group I. The rabbits (group II received to the feed in the same way of 70 °alcohol solution in the same volume. The control group rabbits received the standard feed in the same volume. The feed eating by rabbits was exercised daily. The rabbits ate food completely. The rabbits slaughter was carried out in the morning. The blood plasma protein fractions separation was carried out by horizontal electrophoresis in polyacrylamide gel (PAAG. Mathematical treatment of the research results worked statistically using the software package Statistica 6.0 and Microsoft Excel for Windows XP. Probability differences was assessed by Student t-test and results considered likely at P ≤ 0.05. Results and discussion. We measured the ratio of blood plasma protein fractions of rabbits, which in addition to the feed fed of natural origin biologically active substances. As a result of research was found that aerosol introduction of the spleen extract to the rabbits

  4. STUDIES ON BLOOD GLUTATHIONE PEROXIDASE PROTEIN LEVEL OF CHILDREN IN KESHAN DISEASE AND KASHIN-BECK DISEASE AREAS

    Institute of Scientific and Technical Information of China (English)

    谭武红; 种晓红; 杨占田; 翟连榜; 王立新; 徐光禄

    2002-01-01

    Objective To oberve the change in blood glutathione peroxidase (GSH-Px) protein levels of residents in the low-selenium (Se) area by contrasting the blood GSH-Px protein level of the children in the Keshan disease area with those in the Kashin-Beck disease and non-endemic areas. Methods GSH-Px protein levels were measured by enzyme-linked immunosorbent assays (ELISA). The Se content and GSH-Px activity were assayed by the 2,3-diaminonaphthalene spectrofluorimetric method and glutathione reductase-coupled method respectively. Results ①The blood Se content and GSH-Px protein level of children in Keshan disease area (Moding) were significantly lower than those in Xi'an non-endemic area, however, there was no significant difference when compared with the low-Se non-endemic area; ②The blood Se content, GSH-Px activity and GSH-Px protein level of children in the Kashin-Beck disease area (Yulin) were significantly lower than those of children in two non-endemic areas and in the Keshan disease area; ③The blood Se content and GSH-Px activity were positively correlated to the GSH-Px protein level respectively. Conclusion These results indicate that the blood GSH-Px protein level is decreased in the low-Se residents. The Se status not only affects the GSH-Px activity but also regulate the GSH-Px protein level.

  5. Molecular cloning and protein structure of a human blood group Rh polypeptide

    International Nuclear Information System (INIS)

    cDNA clones encoding a human blood group Rh polypeptide were isolated from a human bone marrow cDNA library by using a polymerase chain reaction-amplified DNA fragment encoding the known common N-terminal region of the Rh proteins. The entire primary structure of the Rh polypeptide has been deduced from the nucleotide sequence of a 1384-base-pair-long cDNA clone. Translation of the open reading frame indicates that the Rh protein is composed of 417 amino acids, including the initiator methionine, which is removed in the mature protein, lacks a cleavable N-terminal sequence, and has no consensus site for potential N-glycosylation. The predicted molecular mass of the protein is 45,500, while that estimated for the Rh protein analyzed in NaDodSO4/polyacrylamide gels is in the range of 30,000-32,000. These findings suggest either that the hydrophobic Rh protein behaves abnormally on NaDodSO4 gels or that the Rh mRNA may encode a precursor protein, which is further matured by a proteolytic cleavage of the C-terminal region of the polypeptide. Hydropathy analysis and secondary structure predictions suggest the presence of 13 membrane-spanning domains, indicating that the Rh polypeptide is highly hydrophobic and deeply buried within the phospholipid bilayer. These results suggest that the expression of the Rh gene(s) might be restricted to tissues or cell lines expressing erythroid characters

  6. Serotonin binding in vitro by releasable proteins from human blood platelets

    International Nuclear Information System (INIS)

    Among the substances released from human blood platelets are serotonin and various proteins. It was hypothesized that one of these proteins binds serotonin and that serotonin might be important to the protein's function or that the protein might be important to serotonin's function. Two platelet-specific proteins, platelet factor 4 (PF4) and β-thromboglobulin (βTG) were found to bind serotonin in vitro. Endogenous PF4 was isolated by serotonin-affinity chromatography and was identified by radioimmunoassay. Purified [125I] -PF4 and native PF4 bound to and eluted from a serotonin-affinity column similarly. Ultrafiltration of the homologous protein, βTG, with [14C]-serotonin demonstrated binding of about 8 moles serotonin per mole tetrameric βTG with a dissociation constant of about 4 X 10(sup-8) M. Equilibrium dialysis of PF4 with radiolabelled serotonin was attempted, but no binding constant values were obtained because serotonin apparently bound to the dialysis membrane. Since EDTA was one of the two agents that eluted PF4 from the serotonin-affinity gel, calcium binding by PF4 was investigated by equilibrium dialysis. Evidence was obtained for positively cooperative binding of calcium ions by PF4. It is concluded that PF4 and βTG bind serotonin in vitro, that they may also bind in vivo when platelets undergo release, and that the functions of serotonin, PF4 and βTG may be mediated in part by serotonin-protein associations

  7. Influence of heparin and thromboplastin in half-life of 125I-protein C in rat blood

    International Nuclear Information System (INIS)

    The influence of heparin and thromboplastin on the halflife of 125I-protein C in rat blood was under investigation. The intravenous administration of heparin resulted in the prolongation of t1/2 to 6.5 h, that could be explained by inhibition of thrombin generation. Upon the 40-min infusion of thromboplastin the rate of 125I-protein C decay in blood enhanced. That could be explained by the generation of the endogenous thrombin and participation of thrombomodulin in the protein C activation as well as in the removal of the endogenous thrombin from blood

  8. Erythrocyte-derived microparticles supporting activated protein C-mediated regulation of blood coagulation.

    Directory of Open Access Journals (Sweden)

    Ruzica Livaja Koshiar

    Full Text Available Elevated levels of erythrocyte-derived microparticles are present in the circulation in medical conditions affecting the red blood cells. Erythrocyte-derived microparticles expose phosphatidylserine thus providing a suitable surface for procoagulant reactions leading to thrombin formation via the tenase and prothrombinase complexes. Patients with elevated levels of circulating erythrocyte-derived microparticles have increased thrombin generation in vivo. The aim of the present study was to investigate whether erythrocyte-derived microparticles are able to support the anticoagulant reactions of the protein C system. Erythrocyte-derived microparticles were isolated using ultracentrifugation after incubation of freshly prepared erythrocytes with the ionophore A23187 or from outdated erythrocyte concentrates, the different microparticles preparations yielding similar results. According to flow cytometry analysis, the microparticles exposed phoshatidylserine and bound lactadherin, annexin V, and protein S, which is a cofactor to activated protein C. The microparticles were able to assemble the tenase and prothrombinase complexes and to stimulate the formation of thrombin in plasma-based thrombin generation assay both in presence and absence of added tissue factor. The addition of activated protein C in the thrombin generation assay inhibited thrombin generation in a dose-dependent fashion. The anticoagulant effect of activated protein C in the thrombin generation assay was inhibited by a monoclonal antibody that prevents binding of protein S to microparticles and also attenuated by anti-TFPI antibodies. In the presence of erythrocyte-derived microparticles, activated protein C inhibited tenase and prothrombinase by degrading the cofactors FVIIIa and FVa, respectively. Protein S stimulated the Arg306-cleavage in FVa, whereas efficient inhibition of FVIIIa depended on the synergistic cofactor activity of protein S and FV. In summary, the erythrocyte

  9. Plasma protein thiols, ceruloplasmin, C-reactive protein and red blood cell acetylcholinesterase in patients undergoing intrauterine insemination

    Directory of Open Access Journals (Sweden)

    Krishnananda Prabhu

    2009-01-01

    Full Text Available Objective: To estimate acetylcholinesterase (AChE, protein thiols (PT, ceruloplasmin (CP and C-reactive proteins (CRPs to assess any change in their levels following intrauterine insemination (IUI. Materials and Methods: Forty-two patients aged 31 ± 4.65 years (mean ± SD with primary infertility selected for IUI. All of them had induced ovulation with clomiphene citrate 50 mg from day 2 to day 6. After taking the consent, 2 ml of blood was withdrawn before and after 24 h of IUI for biochemical estimations. Results: We observed a significant decrease in plasma CP, PT and RBC AChE ( P < 0.001 following IUI compared with the respective pre-procedure levels. Highly sensitive CRP showed a marginal increase after IUI. Conclusion: Fluctuations in levels of the above parameters point to their role in the female reproductive system and in the outcome of the IUI.

  10. Study of p53 protein expression levels from irradiated peripheral blood lymphocytes for biodosimetry

    Energy Technology Data Exchange (ETDEWEB)

    Cavalcanti, M.B.; Fernandes, T.S. [Universidade Federal de Pernambuco (UFPE), Recife, PE (Brazil). Dept. de Energia Nuclear; Amaral, A. [Universite Paris XII (UPXII) (France); Melo, J.A. [Centro de Radioterapia de Pernambuco (CERAPE), PE (Brazil); Neves, M.A.B.; Machado, C.G.F, E-mail: maribrayner@yahoo.com.br [Fundacao de Hematologia e Hemoterapia de Pernambuco, PE (Brazil)

    2005-07-01

    Biodosimetry can be defined as the investigation of radioinduced biological effects in order to correlate them with the absorbed dose. Scoring of unstable chromosomal aberrations and micronuclei, from in vitro irradiated peripheral blood lymphocytes, is commonly used for biodosimetry based on cytogenetic analysis. However, this method of analysis is time-consuming, which may represent a pitfall when fast investigation of a possible exposure to ionizing radiation (IR) is needed. The interaction of IR with the living cell can cause injuries in the DNA molecules. However, normal cells possess mechanisms of repair that are capable to correct those damages. During the repair process of the DNA various proteins are expressed. Among these proteins, p53 plays an important role. This protein is a transcription factor that helps in the maintenance of the genomic integrity. p53 protein is found into the cytoplasm in reduced concentrations and has a short average life. However, expression of p53 protein can be induced by DNA harmful radioinduced, which increases the concentration and the average life of this protein, making possible its detection. Thus, the correlation between the increasing of p53 expression and the irradiation may constitute a fast and reliable method of individual monitoring in cases of accidental or suspected exposures to IR. In this context, the objective of this research was to evaluate the p53 protein expression levels from lymphocytes of the human peripheral blood after in vitro irradiation. For this, samples of peripheral blood from healthy individuals were irradiated with known doses. Lymphocytes were separated on ficoll gradient by centrifugation and re-suspended at 1x 10{sub 6}/mL in RPMI medium enriched with fetal calf serum. Hence, lymphocytes were incubated in 5% CO{sub 2} at 37 deg C prior to the methodology of flow cytometry, using intranuclear antigens for the quantification of p53. In this report, the methodology performed and the results

  11. Pharmaceutical protein production by yeast: towards production of human blood proteins by microbial fermentation

    OpenAIRE

    Martínez, José L.; Liu, Lifang; Petranovic, Dina; Nielsen, Jens

    2012-01-01

    Since the approval of recombinant insulin from Escherichia coli for its clinical use in the early 1980s, the amount of recombinant pharmaceutical proteins obtained by microbial fermentations has significantly increased. The recent advances in genomics together with high throughput analysis techniques (the so-called - omics approaches) and integrative approaches (systems biology) allow the development of novel microbial cell factories as valuable platforms for large scale production of therape...

  12. Regional blood flow in rats after a single low-protein, high-carbohydrate test meal.

    Science.gov (United States)

    Glick, Z; Wickler, S J; Stern, J S; Horwitz, B A

    1984-07-01

    It was previously observed that a single low-protein, high-carbohydrate test meal results in increased in vitro thermic activity of brown adipose tissue. In the present study, we have examined whether such a meal increases the in vivo thermic activity, estimated from measurement of the rate of blood flow. With radioactively labeled microspheres, blood flows into brown fat and several other tissues were determined in meal-deprived (n = 11) and meal-fed (n = 11) rats. The microspheres were injected into the heart of anesthetized animals about 2-2.5 h after the test meal, one injection in the resting state and one during maximal norepinephrine stimulation. In the resting state, blood flow per gram tissue more than doubled in the brown fat (P less than 0.05) and was increased more than 50% in the heart (P less than 0.01) of the fed group. Blood flows into liver and retroperitoneal white fat were reduced by 40 (P less than 0.01) and 30%, respectively, in the fed group. During norepinephrine infusion, significant meal-associated increases in blood flow were evident only in brown fat (P less than 0.05) and the soleus muscle (P less than 0.05), whereas a significant decrease was observed in the liver (P less than 0.05). No statistically significant meal-associated changes in norepinephrine-stimulated blood flow were found in the other tissues examined (i.e., heart, gastrocnemius, and diaphragm muscles, kidneys, white fat, spleen, and adrenals). Our in vivo data thus support the view that brown fat plays a role in the thermic effect of a meal. PMID:6742226

  13. Are Blood-Based Protein Biomarkers for Alzheimer's Disease also Involved in Other Brain Disorders?:A Systematic Review

    OpenAIRE

    Chiam, Justin Tao Wen; Dobson, Richard James Butler; Kiddle, Steven John; Sattlecker, Martina

    2015-01-01

    Background: Alzheimer's disease (AD) biomarkers are urgently needed for both early and accurate diagnosis and prediction of disease progression. Past research has studied blood-based proteins as potential AD biomarkers, revealing many candidate proteins. To date only limited effort has been made to investigate the disease specificity of AD candidate proteins and whether these proteins are also involved in other neurodegenerative or psychiatric conditions.Objective: This review seeks to determ...

  14. Blood serum components and serum protein test of Hybro-PG broilers of different ages

    Directory of Open Access Journals (Sweden)

    PRL Silva

    2007-12-01

    Full Text Available Blood serum samples of HYBRO PG broilers were analyzed, with 30 samples collected from 21-day-old broilers (G1, 30 from 35-day-old birds (G2, and 30 from 42-day-old birds (G3, with the aim of establishing normal values of some blood serum parameters. The activities of the enzymes gamma-glutamyl-transferase (GGT, aspartate aminotransferase (AST, creatine kinase (CK, alkaline phosphatase (ALP, and lactate dehydrogenase (LDH, serum levels of total calcium, calcium ion, phosphorus, sodium, potassium, magnesium, chlorides, creatinine, uric acid, triglycerides, cholesterol, total protein, albumin, total and indirect and direct bilirubin, and electrophoretic profile of serum proteins in acrylamide (SDS-PAGE and agarose gel were determined. There was no influence of age on total bilirubin and albumin levels. All the other evaluated parameters presented differences in at least one age group. Protein electrophoretic profile also changed as a function of age. The obtained results can be considered as normal for the studied ages, and therefore be used as references for the interpretation of laboratory exams of broilers of this genetic line in the evaluated ages.

  15. Cord blood CD4+ T cells respond to self heat shock protein 60 (HSP60.

    Directory of Open Access Journals (Sweden)

    Joost A Aalberse

    Full Text Available BACKGROUND: To prevent harmful autoimmunity most immune responses to self proteins are controlled by central and peripheral tolerance. T cells specific for a limited set of self-proteins such as human heat shock protein 60 (HSP60 may contribute to peripheral tolerance. It is not known whether HSP60-specific T cells are present at birth and thus may play a role in neonatal tolerance. We studied whether self-HSP60 reactive T cells are present in cord blood, and if so, what phenotype these cells have. METHODOLOGY/PRINCIPAL FINDINGS: Cord blood mononuclear cells (CBMC of healthy, full term neonates (n = 21, were cultured with HSP60 and Tetanus Toxoid (TT to study antigen specific proliferation, cytokine secretion and up-regulation of surface markers. The functional capacity of HSP60-induced T cells was determined with in vitro suppression assays. Stimulation of CBMC with HSP60 led to CD4(+ T cell proliferation and the production of various cytokines, most notably IL-10, Interferon-gamma, and IL-6. HSP60-induced T cells expressed FOXP3 and suppressed effector T cell responses in vitro. CONCLUSION: Self-reactive HSP60 specific T cells are already present at birth. Upon stimulation with self-HSP60 these cells proliferate, produce cytokines and express FOXP3. These cells function as suppressor cells in vitro and thus they may be involved in the regulation of neonatal immune responses.

  16. Merozoite surface proteins in red blood cell invasion, immunity and vaccines against malaria.

    Science.gov (United States)

    Beeson, James G; Drew, Damien R; Boyle, Michelle J; Feng, Gaoqian; Fowkes, Freya J I; Richards, Jack S

    2016-05-01

    Malaria accounts for an enormous burden of disease globally, with Plasmodium falciparum accounting for the majority of malaria, and P. vivax being a second important cause, especially in Asia, the Americas and the Pacific. During infection with Plasmodium spp., the merozoite form of the parasite invades red blood cells and replicates inside them. It is during the blood-stage of infection that malaria disease occurs and, therefore, understanding merozoite invasion, host immune responses to merozoite surface antigens, and targeting merozoite surface proteins and invasion ligands by novel vaccines and therapeutics have been important areas of research. Merozoite invasion involves multiple interactions and events, and substantial processing of merozoite surface proteins occurs before, during and after invasion. The merozoite surface is highly complex, presenting a multitude of antigens to the immune system. This complexity has proved challenging to our efforts to understand merozoite invasion and malaria immunity, and to developing merozoite antigens as malaria vaccines. In recent years, there has been major progress in this field, and several merozoite surface proteins show strong potential as malaria vaccines. Our current knowledge on this topic is reviewed, highlighting recent advances and research priorities. PMID:26833236

  17. Heterologe Expression von Proteinen aus Ananas comosus zur Aufklärung der pharmakologischen Wirkmechanismen von Bromelain

    OpenAIRE

    Luniak, Nora

    2007-01-01

    Ziel der Arbeit war die Herstellung der pharmakologisch aktiven Komponenten von Bromelain in Form von rekombinanten Proteinen. Mit den bisher angewendeten Trennmethoden ist es jedoch nicht möglich, einzelne Proteine aus dem Gemisch zu isolierten und daher die pharmakologische Wirkung einer Fraktion auf ein Protein zurückzuführen. Die Arbeit beschäftigte sich daher mit der rekombinanten Herstellung von Cysteinproteasen, die die Hauptbestandteile biochemisch und pharmakologisch charakterisierte...

  18. Personalized liposome-protein corona in the blood of breast, gastric and pancreatic cancer patients.

    Science.gov (United States)

    Colapicchioni, Valentina; Tilio, Martina; Digiacomo, Luca; Gambini, Valentina; Palchetti, Sara; Marchini, Cristina; Pozzi, Daniela; Occhipinti, Sergio; Amici, Augusto; Caracciolo, Giulio

    2016-06-01

    When nanoparticles (NPs) are dispersed in a biofluid, they are covered by a protein corona the composition of which strongly depends on the protein source. Recent studies demonstrated that the type of disease has a crucial role in the protein composition of the NP corona with relevant implications on personalized medicine. Proteomic variations frequently occur in cancer with the consequence that the bio-identity of NPs in the blood of cancer patients may differ from that acquired after administration to healthy volunteers. In this study we investigated the correlation between alterations of plasma proteins in breast, gastric and pancreatic cancer and the biological identity of clinically approved AmBisome-like liposomes as determined by a combination of dynamic light scattering, zeta potential analysis, one-dimensional sodium dodecyl sulfate polyacrylamide gel electrophoresis (1D-SDS-PAGE) and semi-quantitative densitometry. While size of liposome-protein complexes was not significantly different between cancer groups, the hard corona from pancreatic cancer patients was significantly less negatively charged. Of note, the hard corona from pancreatic cancer patients was more enriched than those of other cancer types this enrichment being most likely due to IgA and IgG with possible correlations with the autoantibodies productions in cancer. Given the strict relationship between tumor antigen-specific autoantibodies and early cancer detection, our results could be the basis for the development of novel nanoparticle-corona-based screening tests of cancer. PMID:26369869

  19. Distribution of von Willebrand factor levels in young women with and without bleeding symptoms: influence of ABO blood group and promoter haplotypes

    DEFF Research Database (Denmark)

    Lethagen, S.; Hillarp, A.; Ekholm, C.;

    2008-01-01

    . It was the objective of the present study to evaluate the distribution of VWF levels in young females with or without bleeding symptoms in this population, and the influence of ABO blood group and promoter haplotypes on VWF levels and to identify a possible increased prevalence of VWD in females with bleeding symptoms.......4%) (p = 0.017). Blood group O was found in 14/18 girls with low VWF:RCo. There was a highly significant correlation between VWF:RCo and blood group O and non-O genotypes. Two common VWF promoter haplotypes did not contribute to the VWF:RCo variation. VWF levels did not correlate with time during...... menstrual cycle, or the use of oral contraceptives. No case fulfilled the diagnostic criteria for VWD. In conclusion, low VWF:RCo was significantly more frequent in females with bleeding symptoms. However, we found no case fulfilling strict diagnostic criteria for VWD. The ABO blood group was a strong...

  20. Cobalt inhibits the interaction between hypoxia-inducible factor-alpha and von Hippel-Lindau protein by direct binding to hypoxia-inducible factor-alpha.

    Science.gov (United States)

    Yuan, Yong; Hilliard, George; Ferguson, Tsuneo; Millhorn, David E

    2003-05-01

    The hypoxia-inducible factor (HIF) activates the expression of genes that contain a hypoxia response element. The alpha-subunits of the HIF transcription factors are degraded by proteasomal pathways during normoxia but are stabilized under hypoxic conditions. The von Hippel-Lindau protein (pVHL) mediates the ubiquitination and rapid degradation of HIF-alpha (including HIF-1alpha and HIF-2alpha). Post-translational hydroxylation of a proline residue in the oxygen-dependent degradation (ODD) domain of HIF-alpha is required for the interaction between HIF and VHL. It has previously been established that cobalt mimics hypoxia and causes accumulation of HIF-1alpha and HIF-2alpha. However, little is known about the mechanism by which this occurs. In an earlier study, we demonstrated that cobalt binds directly to the ODD domain of HIF-2alpha. Here we provide the first evidence that cobalt inhibits pVHL binding to HIF-alpha even when HIF-alpha is hydroxylated. Deletion of 17 amino acids within the ODD domain of HIF-2alpha that are required for pVHL binding prevented the binding of cobalt and stabilized HIF-2alpha during normoxia. These findings show that cobalt mimics hypoxia, at least in part, by occupying the VHL-binding domain of HIF-alpha and thereby preventing the degradation of HIF-alpha. PMID:12606543

  1. Biologische Entschwefelung von Biogas

    OpenAIRE

    Schneider, Ralf

    2009-01-01

    Ziel dieser Arbeit ist die Erhöhung von Abbauleistung und Stabilität des biologischen Abbaus von Schwefelwasserstoff (H2S) in Biotropfkörpern zur Entschwefelung von Biogas. Für die Nutzung von Biogas ist häufig eine Reduktion des H2S-Gehalts erforderlich. Üblicherweise wird H2S von aeroben Mikroorganismen bei Zugabe von Luft zum Biogas entweder direkt im Biogasreaktor oder in nachgeschalteten Biotropfkörpern zu Schwefel und Schwefelsäure oxidiert. In der Praxis auftretende Schwankungen der H2...

  2. Effect of source and sex on blood protein fractions of West African Dwarf Goats (WADG

    Directory of Open Access Journals (Sweden)

    J. C. Okonkwo,

    2011-03-01

    Full Text Available Source and sex effects on the total blood protein and its various fractions were studied using juvenile West African Dwarf goats derived from Southern Nigeria. The goats were sourced from three distinct towns in the humid tropics namely, South-East (Umuahia, South-South (Ugheli and South-West (Akure at the rate of 6 males and 18 females per location. The mean values of the total blood plasma protein and its fractions obtained for the WADGs from different zones are 10.01±0.07 g/100ml, 10.07±0.08 g/100ml and 10.16±0.35 g/100ml (total plasma protein; 9.62±0.10 g/100ml, 9.68±0.08 g/100ml and 9.68±0.09 g/100ml (total serum protein, 0.38±0.03 g/100ml, 0.39±0.01 g/100ml, and 0.38±0.04 g/100ml (plasma fibrinogen, 5.62±0.23 g/100ml, 5.78±0.24 g/100ml and 5.45±0.26 g/100ml (serum albumin, 4.00±0.19 g/100ml, 3.89±0.29 g/100ml, and 4.12±0.25 g/100ml (serum globulin, and 1.41±0.27, 1.49±0.15 and 1.34±0.12 (albumin/globulin ratio for the goats from South-East (Umuahia, South-South (Ugheli and South-West (Akure respectively. The studies also indicate that albumin accounts for 53-58% of the total serum protein; globulin accounts for 42-47% serum protein, and the plasma fibrinogen 3.6-4% of the total plasma protein. sex and source interaction had no significant (P>0.05 effects on serum proteins; plasma fibrinogen is sex dependent, and the source of goat affects the proportions of the serum albumin, globulin, and albumin/globulin ratio characteristics of the experimental goats.

  3. Acute responses of muscle protein metabolism to reduced blood flow reflect metabolic priorities for homeostasis.

    Science.gov (United States)

    Zhang, Xiao-Jun; Irtun, Oivind; Chinkes, David L; Wolfe, Robert R

    2008-03-01

    The present experiment was designed to measure the synthetic and breakdown rates of muscle protein in the hindlimb of rabbits with or without clamping the femoral artery. l-[ring-(13)C(6)]phenylalanine was infused as a tracer for measurement of muscle protein kinetics by means of an arteriovenous model, tracer incorporation, and tracee release methods. The ultrasonic flowmeter, dye dilution, and microsphere methods were used to determine the flow rates in the femoral artery, in the leg, and in muscle capillary, respectively. The femoral artery flow accounted for 65% of leg flow. A 50% reduction in the femoral artery flow reduced leg flow by 28% and nutritive flow by 26%, which did not change protein synthetic or breakdown rate in leg muscle. Full clamp of the femoral artery reduced leg flow by 42% and nutritive flow by 59%, which decreased (P < 0.05) both the fractional synthetic rate from 0.19 +/- 0.05 to 0.14 +/- 0.03%/day and fractional breakdown rate from 0.28 +/- 0.07 to 0.23 +/- 0.09%/day of muscle protein. Neither the partial nor full clamp reduced (P = 0.27-0.39) the intracellular phenylalanine concentration or net protein balance in leg muscle. We conclude that the flow threshold to cause a fall of protein turnover rate in leg muscle was a reduction of 30-40% of the leg flow. The acute responses of muscle protein kinetics to the reductions in blood flow reflected the metabolic priorities to maintain muscle homeostasis. These findings cannot be extrapolated to more chronic conditions without experimental validation. PMID:18089763

  4. Vegetarian diet components, protein and blood pressure: which nutrients are important?

    Science.gov (United States)

    Beilin, L J; Burke, V

    1995-03-01

    1. Evidence that vegetarian dietary patterns lower blood pressure (BP) comes from both population studies and randomized controlled trials in normotensive and hypertensive subjects. 2. The effect has been shown most clearly in those who keep to a strict lacto-ovo vegetarian diet characterized by a relatively low intake of saturated fat, a high polyunsaturated/saturated fat ratio, and a high intake of fruit, vegetables and other fibre containing products. Randomized controlled dietary trials suggest the effects are independent of dietary sodium, additive to that of calorie restriction, and not due to the absence of meat protein per se. Indeed, recent population studies suggest an inverse relationship between dietary protein and BP. 4. Dietary fats, fibre, potassium, magnesium and calcium do not independently seem to account for the effects. A possible role for complex carbohydrate in conjunction with the other dietary factors has yet to be fully explored. PMID:7554413

  5. Coagulation Factor and Hemostatic Protein Content of Canine Plasma after Storage of Whole Blood at Ambient Temperature

    OpenAIRE

    Walton, J.E.; Hale, A. S.; Brooks, M. B.; Boag, A.K.; Barnett, W.; Dean, R.

    2014-01-01

    Background Standard practice in canine blood banking is to produce fresh frozen plasma (FFP) by separating and freezing plasma produced from blood within 8 hours of collection. Within canine blood donation programs, this can limit the number of units collected. Hypothesis/Objectives The aim was to compare the coagulation factor and hemostatic protein content (CF&HPC) of plasma produced from blood stored at ambient temperature for 8, 12, and 24 hours. Another aim was to compare the CF&HPC betw...

  6. Modeling of band-3 protein diffusion in the normal and defective red blood cell membrane.

    Science.gov (United States)

    Li, He; Zhang, Yihao; Ha, Vi; Lykotrafitis, George

    2016-04-13

    We employ a two-component red blood cell (RBC) membrane model to simulate lateral diffusion of band-3 proteins in the normal RBC and in the RBC with defective membrane proteins. The defects reduce the connectivity between the lipid bilayer and the membrane skeleton (vertical connectivity), or the connectivity of the membrane skeleton itself (horizontal connectivity), and are associated with the blood disorders of hereditary spherocytosis (HS) and hereditary elliptocytosis (HE) respectively. Initially, we demonstrate that the cytoskeleton limits band-3 lateral mobility by measuring the band-3 macroscopic diffusion coefficients in the normal RBC membrane and in a lipid bilayer without the cytoskeleton. Then, we study band-3 diffusion in the defective RBC membrane and quantify the relation between band-3 diffusion coefficients and percentage of protein defects in HE RBCs. In addition, we illustrate that at low spectrin network connectivity (horizontal connectivity) band-3 subdiffusion can be approximated as anomalous diffusion, while at high horizontal connectivity band-3 diffusion is characterized as confined diffusion. Our simulations show that the band-3 anomalous diffusion exponent depends on the percentage of protein defects in the membrane cytoskeleton. We also confirm that the introduction of attraction between the lipid bilayer and the spectrin network reduces band-3 diffusion, but we show that this reduction is lower than predicted by the percolation theory. Furthermore, we predict that the attractive force between the spectrin filament and the lipid bilayer is at least 20 times smaller than the binding forces at band-3 and glycophorin C, the two major membrane binding sites. Finally, we explore diffusion of band-3 particles in the RBC membrane with defects related to vertical connectivity. We demonstrate that in this case band-3 diffusion can be approximated as confined diffusion for all attraction levels between the spectrin network and the lipid bilayer

  7. Expression of P53(v) protein of peripheral blood in patients with tumor and its relation with age

    International Nuclear Information System (INIS)

    To study the expression of P53(v) protein of peripheral blood monocytes in cancer patients and its relation with age, P53(v) proteins were determined in 88 cancer patients and 88 normals by flow cytometry. The levels of P53(v) were (7.76 +- 7.13)% in cancer group and (0.66 +- 0.5)% in normal group. Mean value of P53(v) in the cancers was higher than that in the controls (P<0.01). The expression of P53(v) of peripheral blood raised with age. Conclusion: P53(v) proteins can be detected in peripheral blood monocytes. The detection of P53 expression in human peripheral blood monocytes can be used to screen high risk population with tumor

  8. Time-evolution of in vivo protein corona onto blood-circulating PEGylated liposomal doxorubicin (DOXIL) nanoparticles

    Science.gov (United States)

    Hadjidemetriou, Marilena; Al-Ahmady, Zahraa; Kostarelos, Kostas

    2016-03-01

    Nanoparticles (NPs) are instantly modified once injected in the bloodstream because of their interaction with the blood components. The spontaneous coating of NPs by proteins, once in contact with biological fluids, has been termed the `protein corona' and it is considered to be a determinant factor for the pharmacological, toxicological and therapeutic profile of NPs. Protein exposure time is thought to greatly influence the composition of protein corona, however the dynamics of protein interactions under realistic, in vivo conditions remain unexplored. The aim of this study was to quantitatively and qualitatively investigate the time evolution of in vivo protein corona, formed onto blood circulating, clinically used, PEGylated liposomal doxorubicin. Protein adsorption profiles were determined 10 min, 1 h and 3 h post-injection of liposomes into CD-1 mice. The results demonstrated that a complex protein corona was formed as early as 10 min post-injection. Even though the total amount of protein adsorbed did not significantly change over time, the fluctuation of protein abundances observed indicated highly dynamic protein binding kinetics.Nanoparticles (NPs) are instantly modified once injected in the bloodstream because of their interaction with the blood components. The spontaneous coating of NPs by proteins, once in contact with biological fluids, has been termed the `protein corona' and it is considered to be a determinant factor for the pharmacological, toxicological and therapeutic profile of NPs. Protein exposure time is thought to greatly influence the composition of protein corona, however the dynamics of protein interactions under realistic, in vivo conditions remain unexplored. The aim of this study was to quantitatively and qualitatively investigate the time evolution of in vivo protein corona, formed onto blood circulating, clinically used, PEGylated liposomal doxorubicin. Protein adsorption profiles were determined 10 min, 1 h and 3 h post

  9. Myeloperoxidase activity and the oxidized proteins in blood neutrophils of patients with pneumonia.

    Science.gov (United States)

    Muravlyova, Larissa; Molotov-Luchanskiy, Vilen; Bakirova, Ryszhan; Klyuyev, Dmitriy; Demidchik, Ludmila; Kolesnikova, Yevgeniya

    2014-10-01

    The main purpose of our investigation was to study myeloperoxidase activity and concentration of oxidized proteins in blood neutrophils of patients with ambulant pneumonia and secondary pneumonia which has arisen on a background of chronic obstructive pulmonary disease (COPD). Patients were divided into 2 groups. 17 patients with ambulant pneumonia moderate severity and respiratory insufficiency of grade 2 were included in the 1-st group. 20 COPD patients with secondary pneumonia moderate severity and with respiratory insufficiency of grade 2 were included in the 2-nd group. The control group consisted of 15 healthy subjects. The reactive protein carbonyl derivates, advanced oxidation protein products (AOPP) and myeloperoxidase activity were detected in neutrophils. In neutrophils of 1-st group patients the augmentation of reactive protein carbonyl derivates was observed in comparison with healthy ones. In neutrophils of 2-nd group patients the slight decrease of reactive protein carbonyl derivates was observed in comparison with healthy ones (by 17%). In neutrophils of 2-nd group patients the significant increasing AOPP in comparison with healthy ones (p <0.01) and 1 group patients (p <0.05) was fixed. Myeloperoxidase activity was higher in neutrophils of 1-th group patients in comparison with healthy ones. In neutrophils of 2-nd group patients myeloperoxidase activity was higher in comparison with the same of 1 group patients (by 67%, p <0.05). Our results showed the different direction of oxidized proteins formation neutrophils of patients with primary and secondary pneumonia. Besides that the varied degree of myeloperoxidase activity was fixed. Our results require more detailed understanding because they can reflect peculiar mechanisms of pneumonia development and determine the characteristics of their progression. PMID:26461373

  10. Untersuchung zur optischen Regulation von elektrischer Leitfähigkeit in DNA und Synthese neuer photolabiler Schutzgruppen für Protein- und Oligonukleotidanwendungen

    OpenAIRE

    Friedrich, Felix

    2015-01-01

    Im Rahmen der vorliegenden Arbeit wurde einerseits der Einsatz lichtaktivierbarer Oligonukleotide zur Kontrolle der Leitfähigkeit entlang von DNA untersucht sowie neue photoaktivierbare Verbindungen für die Peptidchemie und für eine neu entwickelte Variante des SELEX (Systematic Evolution of Ligands by EXponetial enrichment) Verfahrens synthetisiert. DNA vermittelte Ladungsübertragung verläuft entlang des gestapelten π-Systems der heteroaromatischen Nukleobasen. Die Leitfähigkeit von Olig...

  11. Interaktive Visualisierung von Strukturmechaniksimulationen

    OpenAIRE

    Sommer, Ove

    2003-01-01

    A) Motivation und Problemstellung Der Computer ist heute eines der wichtigsten Hilfsmittel beim Entwurf und der Entwicklung von Fahrzeugen. Zunächst wurde er von Konstrukteuren für das Computer Aided Design (CAD) von virtuellen Fahrzeugmodellen eingesetzt. Inzwischen ist er in vielen anderen Bereichen der Fahrzeugentwicklung unentbehrlich geworden: der Entwicklungszyklus von Automobilen ist durch die Computer-gestützte numerische Simulation substanziell verkürzt worden. An virtuellen Prot...

  12. Carl von Ossietzky

    OpenAIRE

    Suhr, Elke; Ossietzky-Palm, Rosalinda von

    1988-01-01

    Ausstellung und Katalog wurden von Mitarbeitern der Universität Oldenburg anläßlich des 50. Todestages Carl von Ossietzkys erarbeitet. Diese dritte Ossietzky-Ausstellung in Oldenburg steht unter dem Thema "Republikaner ohne Republik". Der Katalog gibt zahlreiche Schautafeln der Ausstellung wieder und enthält ferner kürzere Textbeiträge verschiedener Autoren, darunter auch Erinnerungen von Zeitgenossen Carl von Ossietzkys.

  13. Observational and mechanistic links between C-reactive protein and blood pressure in elderly women.

    Science.gov (United States)

    Hosford-Donovan, Adrian; Nilsson, Andreas; Wåhlin-Larsson, Britta; Kadi, Fawzi

    2016-07-01

    It is hypothesized that chronic systemic inflammation contributes to the age-related decline in cardiovascular function. The aim of the present study was to combine an assessment of the relationship between the serum level of C-reactive protein (CRP) and systolic and diastolic blood pressure in 108 elderly women (65 and 70 years) with an in-vitro exploration of the effects of CRP on the proliferative and angiogenic potential of endothelial cells exposed to serum in elderly women. Based on the median CRP level in our population, LowCRP (CRP1.3mg/L) groups were identified. Body mass index, waist circumference, systolic blood pressure (SBP) and diastolic blood pressure (DBP) were significantly higher in the HighCRP group than in the LowCRP group (pCRP on SBP and DBP remained significant after adjustments for BMI and use of antihypertensive medication (pCRP on SPB was attenuated (p=0.062). We next evaluated the ability to form capillary tubes (angiogenesis assay) and the proliferation rate of endothelial cells exposed to the sera of elderly women. Increased serum CRP levels were associated with an increased doubling time of endothelial cells (R(2)=0.39; p<0.05) and decreased capillary tube length (R(2)=0.30; p<0.05), indicating a reduction in the proliferation rate of endothelial cells and angiogenic potential. In conclusion, chronic inflammation influences blood pressure in elderly women and compromises endothelial cell function, thus contributing to the age-related decline in vascular health. PMID:27180160

  14. Blood levels of glial fibrillary acidic protein (GFAP in patients with neurological diseases.

    Directory of Open Access Journals (Sweden)

    Christoph A Mayer

    Full Text Available BACKGROUND AND PURPOSE: The brain-specific astroglial protein GFAP is a blood biomarker candidate indicative of intracerebral hemorrhage in patients with symptoms suspicious of acute stroke. Comparably little, however, is known about GFAP release in other neurological disorders. In order to identify potential "specificity gaps" of a future GFAP test used to diagnose intracerebral hemorrhage, we measured GFAP in the blood of a large and rather unselected collective of patients with neurological diseases. METHODS: Within a one-year period, we randomly selected in-patients of our university hospital for study inclusion. Patients with ischemic stroke, transient ischemic attack and intracerebral hemorrhage were excluded. Primary endpoint was the ICD-10 coded diagnosis reached at discharge. During hospital stay, blood was collected, and GFAP plasma levels were determined using an advanced prototype immunoassay at Roche Diagnostics. RESULTS: A total of 331 patients were included, covering a broad spectrum of neurological diseases. GFAP levels were low in the vast majority of patients, with 98.5% of cases lying below the cut-off that was previously defined for the differentiation of intracerebral hemorrhage and ischemic stroke. No diagnosis or group of diagnoses was identified that showed consistently increased GFAP values. No association with age and sex was found. CONCLUSION: Most acute and chronic neurological diseases, including typical stroke mimics, are not associated with detectable GFAP levels in the bloodstream. Our findings underline the hypothesis that rapid astroglial destruction as in acute intracerebral hemorrhage is mandatory for GFAP increase. A future GFAP blood test applied to identify patients with intracerebral hemorrhage is likely to have a high specificity.

  15. Biochemical and Functional Analysis of Two Plasmodium falciparum Blood-Stage 6-Cys Proteins: P12 and P41

    OpenAIRE

    Tana Taechalertpaisarn; Cecile Crosnier; S Josefin Bartholdson; Hodder, Anthony N.; Jenny Thompson; Bustamante, Leyla Y.; Wilson, Danny W.; Sanders, Paul R.; Wright, Gavin J.; Rayner, Julian C.; Cowman, Alan F.; Gilson, Paul R.; Crabb, Brendan S

    2012-01-01

    The genomes of Plasmodium parasites that cause malaria in humans, other primates, birds, and rodents all encode multiple 6-cys proteins. Distinct 6-cys protein family members reside on the surface at each extracellular life cycle stage and those on the surface of liver infective and sexual stages have been shown to play important roles in hepatocyte growth and fertilization respectively. However, 6-cys proteins associated with the blood-stage forms of the parasite have no known function. Here...

  16. Elucidation of binding mechanism and identification of binding site for an anti HIV drug, stavudine on human blood proteins.

    Science.gov (United States)

    Sandhya, B; Hegde, Ashwini H; Seetharamappa, J

    2013-05-01

    The binding of stavudine (STV) to two human blood proteins [human hemoglobin (HHb) and human serum albumin (HSA)] was studied in vitro under simulated physiological conditions by spectroscopic methods viz., fluorescence, UV absorption, resonance light scattering, synchronous fluorescence, circular dichroism (CD) and three-dimensional fluorescence. The binding parameters of STV-blood protein were determined from fluorescence quenching studies. Stern-Volmer plots indicated the presence of static quenching mechanism in the interaction of STV with blood proteins. The values of n close to unity indicated that one molecule of STV bound to one molecule of blood protein. The binding process was found to be spontaneous. Analysis of thermodynamic parameters revealed the presence of hydrogen bond and van der Waals forces between protein and STV. Displacement experiments indicated the binding of STV to Sudlow's site I on HSA. Secondary structures of blood proteins have undergone changes upon interaction with STV as evident from the reduction of α-helices (from 46.11% in free HHb to 38.34% in STV-HHb, and from 66.44% in free HSA to 52.26% in STV-HSA). Further, the alterations in secondary structures of proteins in the presence of STV were confirmed by synchronous and 3D-fluorescence spectral data. The distance between the blood protein (donor) and acceptor (STV) was found to be 5.211 and 5.402 nm for STV-HHb and STV-HSA, respectively based on Föster's non-radiative energy transfer theory. Effect of some metal ions was also investigated. The fraction of STV bound to HSA was found to be 87.8%. PMID:23275205

  17. Shock, diaschisis and von Monakow

    Directory of Open Access Journals (Sweden)

    Eliasz Engelhardt

    2013-07-01

    Full Text Available The concept of shock apparently emerged in the middle of the 18th century (Whyett as an occurrence observed experimentally after spinal cord transection, and identified as "shock" phenomenon one century later (Hall. The concept was extended (Brown-Séquard and it was suggested that brain lesions caused functional rupture in regions distant from the injured one ("action à distance". The term "diaschisis" (von Monakow, proposed as a new modality of shock, had its concept broadened, underpinned by observations of patients, aiming at distinguishing between symptoms of focal brain lesions and transitory effects they produced, attributable to depression of distant parts of the brain connected to the injured area. Presently, diaschisis is related mainly to cerebrovascular lesions and classified according to the connection fibers involved, as proposed by von Monakow. Depression of metabolism and blood flow in regions anatomically separated, but related by connections with the lesion, allows observing diaschisis with neuroimaging.

  18. [Dynamics of blood proteins spectra in normal and diarrheic long horn cattle during early ontogenesis].

    Science.gov (United States)

    Usatiuk, P V; Tsvilikhovs'kyĭ, M I; Aniiefiok, A; Mel'nychuk, D O

    2001-01-01

    The contains of blood plasma protein fractions and immunoglobulines were studied in adult cattle and in new born calves (just new born and age of 1, 2, 5 and 10 days) by the 10% PAAG EP (0.1% SDS) and radial immunodiffusion methods. It is shown that protein bands of 140-160 kD and immunoglobulines (G1, G2, M) are absent at the moment of birth. The physiology level of gamma-globulines and IgG1 reach at 1 day of life, while IgM--at second. After colostrum breeding of just new born calves the speed increasing of total plasma protein and gamma-globulines fraction are lowest (in 2.7 and 1.7 times respectively) in diarrhoea animals in compare to the health one. The maximum level of IgG1 concentration forms in disease animals only at 10 days of age. On the date obtained it is concluded the presence such new born calves which have low level of small intestine developing and disturbance of immunoglobulines absorption. PMID:11962094

  19. Localization of Cellular Retinol-Binding Protein and Retinol-Binding Protein in Cells Comprising the Blood-Brain Barrier of Rat and Human

    Science.gov (United States)

    MacDonald, Paul N.; Bok, Dean; Ong, David E.

    1990-06-01

    Brain is not generally recognized as an organ that requiries vitamin A, perhaps because no obvious histologic lesions have been observed in severely vitamin A-deficient animals. However, brain tissue does contain cellular vitamin A-binding proteins and a nuclear receptor protein for retinoic acid. In the present study, immunohistochemical techniques were used to determine the cell-specific location of cellular retinol-binding protein in human and rat brain tissue. Cellular retinol-binding protein was localized specifically within the endothelial cells of the brain microvasculature and within the cuboidal epithelial cells of the choroid plexus, two primary sites of the mammalian blood-brain barrier. In addition, autoradiographic procedures demonstrated binding sites for serum retinol-binding protein in the choroidal epithelium. These observations suggest that a significant movement of retinol across the blood-brain barrier may occur.

  20. Localization of cellular retinol-binding protein and retinol-binding protein in cells comprising the blood-brain barrier of rat and human

    Energy Technology Data Exchange (ETDEWEB)

    MacDonald, P.N.; Ong, D.E. (Vanderbilt Univ., Nashville, TN (USA)); Bok, D. (Univ. of California, Los Angeles (USA))

    1990-06-01

    Brain is not generally recognized as an organ that requires vitamin A, perhaps because no obvious histologic lesions have been observed in severely vitamin A-deficient animals. However, brain tissue does contain cellular vitamin A-binding proteins and a nuclear receptor protein for retinoic acid. In the present study, immunohistochemical techniques were used to determine the cell-specific location of cellular retinol-binding protein in human and rat brain tissue. Cellular retinol-binding protein was localized specifically within the cuboidal epithelial cells of the choroid plexus, two primary sites of the mammalian blood-brain barrier. In addition, autoradiographic procedures demonstrated binding sites for serum retinol-binding protein in the choroidal epithelium. These observations suggest that a significant movement of retinol across the blood-brain barrier may occur.

  1. Entwicklung und Anwendung enzymimmunologischer Verfahren zum Nachweis von Alternariol

    OpenAIRE

    Ackermann, Yvonne Christine

    2014-01-01

    Die vorliegende Arbeit beschreibt die Entwicklung und Anwendung von zwei Enzymimmuntests zum Nachweis von Alternariol auf der Basis polyklonaler- bzw. monoklonaler Antikörpern gegen dieses Mykotoxin (pAk- bzw. mAk-EIA). Zur Herstellung von Alternariol-Protein-Konjugaten wurde Alternariol mittels der Mannich- Kondensations-Reaktion an keyhole limpet hemocyanin (KLH) bzw. an bovines Serumalbumin (BSA) gekoppelt. Zur Gewinnung polyklonaler Antikörper wurden Kaninchen mit Alternariol-KLH immunisi...

  2. Extracorporeal irradiation of calves blood. Effects on: the lymphocytes, the blood-platelet function, seric proteins, and fibrinogen

    International Nuclear Information System (INIS)

    The present paper reports the results obtained after extracorporeal irradiation of circulating blood of calves. Animals are divided in 3 groups as follows: - control animals: blood circulation without irradiation; - calves which received 40000 rads during 24 hours of continuous irradiation; - calves which received the same dose, during a period of 5 days (5 hours every day). The more interesting results are: - the early lymphopenia which persists for 7 or 8 weeks and may be in relationship with the change of immunoglobulins; - a constant hyperfibrinemia (12 g/l) never reported, as far as we know, by authors using I.E.C. Several hypothesis are advanced to explain this phenomenon. (authors)

  3. Herstellung und Charakterisierung von Proteinprodukten aus Palerbsen und deren Potential zur Bildung von Proteinmatrices mit hohen Lipidanteilen in Futtermitteln für Salmoniden

    OpenAIRE

    Wild, Florian

    2012-01-01

    Die Steigerung des Austausches von Fischmehl durch alternative Proteinrohstoffe in Fischfuttermitteln ist eine Grundvoraussetzung für ein weiteres Wachstum der Aquakulturwirtschaft, in der die Zucht von Lachs der bedeutendste Produktionszweig ist. Aufgrund der karnivoren Ernährungsweise von Lachs ist dessen Verdauungssystem an die Aufnahme von sehr protein- und fettreicher Nahrung aus seiner natürlichen Umgebung angepasst. Fischfuttermittel für Lachse müssen daher zu möglichst hohen Anteilen ...

  4. Effect of peptides derived from food proteins on blood pressure – a meta-analysis of randomised controlled trials

    OpenAIRE

    Pripp, Are Hugo

    2008-01-01

    Background: Peptides derived from food proteins have in clinical trials shown an effect on blood pressure. Their biological mechanism is mainly due to inhibition of angiotensin-I-converting enzyme (ACE) and thereby regulation of blood pressure through the renin-angiotensin system. A meta-analysis of these trials is needed to better quantify their effect, sources of variation and possible publication bias. Objective: To perform a meta-analysis of placebo-controlled clinical trials on peptides ...

  5. Adsorption and adhesion of blood proteins and fibroblasts on multi-wall carbon nanotubes

    Institute of Scientific and Technical Information of China (English)

    2009-01-01

    This article concerns the investigation of blood protein adsorption on carbon paper and multi-wall carbon nanotubes (MWCNTs). Mouse fibroblast cell adhesion and growth on MWCNTs was also studied. The results showed that fibrinogen adsorption on carbon paper was much lower than that on MWCNTs, which means that platelets readily aggregate on the surface of MWCNTs. Mouse fibroblast cells implanted on MWCNTs tended to grow more prolifically than those implanted on carbon paper. The cell concentration observed on MWCNTs increased from 1.2×105/mL for a single day culture to 2×105/mL for a 7-day culture. No toxicity reaction was observed during the culturing period. These results indicated that MWCNTs possessed excellent tissue compatibility.

  6. Residual nanoparticle label immunosensor for wash-free C-reactive protein detection in blood.

    Science.gov (United States)

    Huttunen, Roope J; Näreoja, Tuomas; Mariani, Laura; Härmä, Harri

    2016-09-15

    Current diagnostic immunotechnologies are universally based on the measurement of the bound label-antibody fraction in direct binding or sandwich-assay type approaches with various detection techniques (e.g. enzyme-linked immunosorbent assay or ELISA) on solid stationary phase surface. Here an alternative reciprocal approach is presented based on the detection of the non-bound fraction of nanoparticle-labelled antibodies using microparticles as solid support. The advantage of detecting the non-bound fraction of the labelled antibody instead of the bound fraction is the high dynamics and the suggested increased flexibility in the selection of the detection mode. No actual washing steps are required as the bound and non-bound fractions of the detection nanoparticle label are separated using physical separation rather than consecutive washing repeats. The quantitative proof-of-concept set-up was demonstrated through blood-based detection of C-reactive protein (CRP). A blood sample containing CRP was diluted 1/50 and measured in 15-min resulting in a linear response at a range from 1 to 30μg/ml. The lowest limit of detection was below 0.03μg/ml and the assay coefficient of variation ranged from 0.3 to 9%. The nanoparticle-based residual label detection outperformed the corresponding molecular label method providing wider applicability with nearly an order of magnitude higher signal-to-background ratio for novel assay configurations in clinical diagnostics practices. PMID:27104585

  7. Regulation of Exacerbated Immune Responses in Human Peripheral Blood Cells by Hydrolysed Egg White Proteins.

    Science.gov (United States)

    Lozano-Ojalvo, Daniel; Molina, Elena; López-Fandiño, Rosina

    2016-01-01

    The anti-allergic potential of egg white protein hydrolysates (from ovalbumin, lysozyme and ovomucoid) was evaluated as their ability to hinder cytokine and IgE production by Th2-skewed human peripheral blood mononuclear cells (PBMCs), as well as the release of pro-inflammatory factors and generation of reactive oxygen species from Th1-stimulated peripheral blood leukocytes (PBLs). The binding to IgE of egg allergic patients was determined and the peptides present in the hydrolysates were identified. The hydrolysates with alcalase down-regulated the production of Th2-biased cytokines and the secretion of IgE to the culture media of Th2-skewed PBMCs, and they significantly neutralized oxidative stress in PBLs. The hydrolysates of ovalbumin and ovomucoid with pepsin helped to re-establish the Th1/Th2 balance in Th2-biased PBMCs, while they also inhibited the release of pro-inflammatory mediators and reduced oxidative stress in PBLs treated with inflammatory stimuli. The hydrolysates with alcalase, in addition to equilibrating Th2 differentiation, exhibited a low IgE-binding. Therefore, they would elicit mild allergic reactions while retaining T cell-stimulating abilities, which might correlate with an anti-allergic benefit. PMID:27007699

  8. Regulation of Exacerbated Immune Responses in Human Peripheral Blood Cells by Hydrolysed Egg White Proteins.

    Directory of Open Access Journals (Sweden)

    Daniel Lozano-Ojalvo

    Full Text Available The anti-allergic potential of egg white protein hydrolysates (from ovalbumin, lysozyme and ovomucoid was evaluated as their ability to hinder cytokine and IgE production by Th2-skewed human peripheral blood mononuclear cells (PBMCs, as well as the release of pro-inflammatory factors and generation of reactive oxygen species from Th1-stimulated peripheral blood leukocytes (PBLs. The binding to IgE of egg allergic patients was determined and the peptides present in the hydrolysates were identified. The hydrolysates with alcalase down-regulated the production of Th2-biased cytokines and the secretion of IgE to the culture media of Th2-skewed PBMCs, and they significantly neutralized oxidative stress in PBLs. The hydrolysates of ovalbumin and ovomucoid with pepsin helped to re-establish the Th1/Th2 balance in Th2-biased PBMCs, while they also inhibited the release of pro-inflammatory mediators and reduced oxidative stress in PBLs treated with inflammatory stimuli. The hydrolysates with alcalase, in addition to equilibrating Th2 differentiation, exhibited a low IgE-binding. Therefore, they would elicit mild allergic reactions while retaining T cell-stimulating abilities, which might correlate with an anti-allergic benefit.

  9. Investigation of two blood proteins binding to Cantharidin and Norcantharidin by multispectroscopic and chemometrics methods

    Energy Technology Data Exchange (ETDEWEB)

    Liu, Rong; Cheng, Zhengjun, E-mail: ncczj1112@126.com; Li, Tian; Jiang, Xiaohui

    2015-01-15

    The interactions of Cantharidin/Norcantharidin (CTD/NCTD) with two blood proteins, i.e., bovine serum albumin (BSA) and bovine hemoglobin (BHb), have been investigated by the fluorescence, UV–vis absorption, and FT-IR spectra under imitated physiological condition. The binding characteristics between CTD/NCTD and BSA/BHb were determined by fluorescence emission and resonance light scattering (RLS) spectra. The quenching mechanism of two blood proteins with CTD/NCTD is a static quenching. Moreover, the experimental data were further analyzed based on multivariate curve resolution-alternating least squares (MCR-ALS) technique to obtain the concentration profiles and pure spectra for three species (BSA/BHb, CTD/NCTD and CTD/NCTD–BSA/BHb complexes) which existed in the interaction procedure. The number of binding sites n and binding constants K{sub b} were calculated at various temperatures. The thermodynamic parameters (such as, ΔG, ΔH, and ΔS) for BSA–CTD/NCTD and BHb–CTD/NCTD systems were calculated by the Van’t Hoff equation and also discussed. The distance r between CTD/NCTD and BSA/BHb were evaluated according to Förster no-radiation energy transfer theory. The results of Fourier transform infrared (FT-IR), synchronous fluorescence and three-dimensional fluorescence spectra showed that the conformations of BSA/BHb altered with the addition of CTD/NCTD. In addition, the effects of common ions on the binding constants of BSA–CTD/NCTD and BHb–CTD/NCTD systems were also discussed.

  10. Effect of dietary protein sources of on blood or milk urea nitrogen of native cows

    International Nuclear Information System (INIS)

    When feed protein metabolism in ruminants produces urea in the liver and recycles or blood urea (BUN) filters into milk urea nitrogen (MUN), an indicator of protein status in diets or feeding urea as one of the non-protein nitrogen sources for ruminants is scientifically acceptable throughout the world; a section of environmentalists, policy makers or even professionals often raise question of residual effects in milk and/or meat of fattening and/or dairy cattle fed with diets containing urea. Keeping their views in consideration, a feeding trial on 30 Pabna milking cows of 2 to 4 parities dividing equally into 5 groups was arranged to determine the effect of feeding of different sources of protein on BUN and MUN, and milk yield or protein content. To achieve the objectives, a group of cows was fed a diet of rice straw and concentrate as the control (T0), two out of the rests was fed either with urea-molasses straw (UMS) (T1) or Matikalai (Vigna mungo) hay ( T2) as sources of basal roughage. The rest two groups of cows were fed the control diet replacing % of feed protein by the amount of urea and molasses fed to UMS group. The amount of urea and molasses was fed daily either in two meals (T3) or fed to cows mixing with other concentrate feed (T4). In addition, a concentrate mixture containing 45 % wheat bran, 24% Khesari bran, 12% Til oil cake, 12% soybean meal, 4% fishmeal, 2.0% oyster-shell, 0.5% DCP and 0.5% common salt, was supplied twice daily. Having adjusted the cows with the diets for 20 d, a 20 d feeding trial was conducted, when feed intake and samples of blood and milk were collected. Milk samples were collected from individual cow after feeding the experimental diets in the morning and evening milking. Samples were collected from milk bucket after complete milking and mixing thoroughly. Samples were analyzed for milk urea content (MUN) using a Colorimetric p-dimethylaminobenzaldehyde (DMAB) method as described by Bector et al. Concentration of MUN in

  11. Electrophoretic pattern of blood serum proteins and enzymes activity of house sparrow (Passer domesticus in Baghdad (Iraq

    Directory of Open Access Journals (Sweden)

    Faris A. Al-Obaidi

    2011-04-01

    Full Text Available The objective of this study was to determine the electrophoretic pattern of blood serum proteins and the activity of some blood serum enzymes included GOT, GPT and AP of house Sparrow (Passer domesticus in Baghdad, Iraq. Seventeen (9 males and 8 females individuals of house sparrow were collected from different regions of Baghdad. Samples of 1.0 ml of whole blood were taken from the wing vein from individuals to determined electrophoretic pattern of serum proteins and enzymes in three replicates for each sex within species. Results revealed that house sparrow blood serum proteins were separated into seven different regions, these bands were pre- albumen (3.68%, albumen (13.20%, post–albumen (8.37%, α–globulin (4.54%, β–globulin (27.49%, γ–globulin (30.15% and transferrin (12.59%. Electrophoretic pattern of serum proteins were differed due to sex: females dominated males in pre–albumen, γ–globulin and total albumens. House sparrow blood serum GOT, GPT and AP enzymes activity were 38.4, 10.9 and 42.05 U/L respectively. Males had higher activity of GOT and GPT enzymes compared to females.

  12. Biochemical and functional analysis of two Plasmodium falciparum blood-stage 6-cys proteins: P12 and P41.

    Directory of Open Access Journals (Sweden)

    Tana Taechalertpaisarn

    Full Text Available The genomes of Plasmodium parasites that cause malaria in humans, other primates, birds, and rodents all encode multiple 6-cys proteins. Distinct 6-cys protein family members reside on the surface at each extracellular life cycle stage and those on the surface of liver infective and sexual stages have been shown to play important roles in hepatocyte growth and fertilization respectively. However, 6-cys proteins associated with the blood-stage forms of the parasite have no known function. Here we investigate the biochemical nature and function of two blood-stage 6-cys proteins in Plasmodium falciparum, the most pathogenic species to afflict humans. We show that native P12 and P41 form a stable heterodimer on the infective merozoite surface and are secreted following invasion, but could find no evidence that this complex mediates erythrocyte-receptor binding. That P12 and P41 do not appear to have a major role as adhesins to erythrocyte receptors was supported by the observation that antisera to these proteins did not substantially inhibit erythrocyte invasion. To investigate other functional roles for these proteins their genes were successfully disrupted in P. falciparum, however P12 and P41 knockout parasites grew at normal rates in vitro and displayed no other obvious phenotypic changes. It now appears likely that these blood-stage 6-cys proteins operate as a pair and play redundant roles either in erythrocyte invasion or in host-immune interactions.

  13. Detection of Antibodies in Blood Plasma Using Bioluminescent Sensor Proteins and a Smartphone.

    Science.gov (United States)

    Arts, Remco; den Hartog, Ilona; Zijlema, Stefan E; Thijssen, Vito; van der Beelen, Stan H E; Merkx, Maarten

    2016-04-19

    Antibody detection is of fundamental importance in many diagnostic and bioanalytical assays, yet current detection techniques tend to be laborious and/or expensive. We present a new sensor platform (LUMABS) based on bioluminescence resonance energy transfer (BRET) that allows detection of antibodies directly in solution using a smartphone as the sole piece of equipment. LUMABS are single-protein sensors that consist of the blue-light emitting luciferase NanoLuc connected via a semiflexible linker to the green fluorescent acceptor protein mNeonGreen, which are kept close together using helper domains. Binding of an antibody to epitope sequences flanking the linker disrupts the interaction between the helper domains, resulting in a large decrease in BRET efficiency. The resulting change in color of the emitted light from green-blue to blue can be detected directly in blood plasma, even at picomolar concentrations of antibody. Moreover, the modular architecture of LUMABS allows changing of target specificity by simple exchange of epitope sequences, as demonstrated here for antibodies against HIV1-p17, hemagglutinin (HA), and dengue virus type I. The combination of sensitive ratiometric bioluminescent detection and the intrinsic modularity of the LUMABS design provides an attractive generic platform for point-of-care antibody detection that avoids the complex liquid handling steps associated with conventional immunoassays. PMID:27018236

  14. Phagocytic and oxidative-burst activity of blood leukocytes in rats fed a protein-free diet

    DEFF Research Database (Denmark)

    Sawosz, Ewa; Winnicka, Anna; Chwalibog, André;

    2009-01-01

    The objective of this study was to evaluate the effects of two weeks' protein deprivation on the cellular parameters of non-specific immunity in rats. Wistar rats (200-250 g) were divided into two groups (2x12) and were fed two isoenergetic (control and protein-free) diets. The phagocytic activity...... of neutrophils and monocytes, and the oxidative-burst activity of neutrophils of peripheral blood, were determined by flow cytometry after stimulation with E. coli and phorbol 12-mirystate 13-acetate. Feeding the protein-free diet for two weeks did not influence the phagocytic activity of neutrophils......, monocytes or blood morphology. However, the oxidative burst of stimulated neutrophils was increased indicating that two weeks' protein deprivation does not depress the oxygen-dependent killing mechanism in neutrophils, but may lead to the overproduction of reactive oxygen species....

  15. Einfluß des surfactantassoziierten Proteins B auf die Aufnahme von Lipiden in Typ II-Zellen im Vergleich mit und ohne das surfactantassoziierte Protein A

    OpenAIRE

    Zerr, Helena

    2010-01-01

    Surfactant (surface active agent) covers the alveoli of the lung and lowers the surface tension on the air-water-interface. Surfactant is a complex mixture and contains about 90% of lipid and 10% of surfactant associated proteins. Specialized lung epithelial cells, the alveolar type II cells, produce surfactant. The cells do secretion and uptake by processes called exo- and endocytosis. For decades native and synthetic preparations of surfactant have been an effective medic...

  16. Blood smear

    Science.gov (United States)

    ... osmotic fragility ) Deficiency of an enzyme called lecithin cholesterol acyl transferase Abnormalities of hemoglobin , the protein in ... sickle and Pappenheimer Red blood cells, target cells Formed elements of blood References Bain BJ. The peripheral ...

  17. Proteomic analysis of ERK1/2-mediated human sickle red blood cell membrane protein phosphorylation

    Directory of Open Access Journals (Sweden)

    Soderblom Erik J

    2013-01-01

    Full Text Available Abstract Background In sickle cell disease (SCD, the mitogen-activated protein kinase (MAPK ERK1/2 is constitutively active and can be inducible by agonist-stimulation only in sickle but not in normal human red blood cells (RBCs. ERK1/2 is involved in activation of ICAM-4-mediated sickle RBC adhesion to the endothelium. However, other effects of the ERK1/2 activation in sickle RBCs leading to the complex SCD pathophysiology, such as alteration of RBC hemorheology are unknown. Results To further characterize global ERK1/2-induced changes in membrane protein phosphorylation within human RBCs, a label-free quantitative phosphoproteomic analysis was applied to sickle and normal RBC membrane ghosts pre-treated with U0126, a specific inhibitor of MEK1/2, the upstream kinase of ERK1/2, in the presence or absence of recombinant active ERK2. Across eight unique treatment groups, 375 phosphopeptides from 155 phosphoproteins were quantified with an average technical coefficient of variation in peak intensity of 19.8%. Sickle RBC treatment with U0126 decreased thirty-six phosphopeptides from twenty-one phosphoproteins involved in regulation of not only RBC shape, flexibility, cell morphology maintenance and adhesion, but also glucose and glutamate transport, cAMP production, degradation of misfolded proteins and receptor ubiquitination. Glycophorin A was the most affected protein in sickle RBCs by this ERK1/2 pathway, which contained 12 unique phosphorylated peptides, suggesting that in addition to its effect on sickle RBC adhesion, increased glycophorin A phosphorylation via the ERK1/2 pathway may also affect glycophorin A interactions with band 3, which could result in decreases in both anion transport by band 3 and band 3 trafficking. The abundance of twelve of the thirty-six phosphopeptides were subsequently increased in normal RBCs co-incubated with recombinant ERK2 and therefore represent specific MEK1/2 phospho-inhibitory targets mediated via ERK2

  18. Effect of whey supplementation on blood markers of protein metabolism in young and elderly after resistance exercise

    OpenAIRE

    Holte, Kristin

    2014-01-01

    Abstract Introduction: Ingestion of whey protein has been shown to be superior to casein in the acute stimulation of anabolic responses in muscle. The composition of whey protein may alter how rapidly the amino acids are available after consumption, and thus affect acute anabolic responses in muscle and other tissues. Aims: To investigate how ingestion of different whey products, influences the acute changes in the blood amino acid and urea concentration following standardized resistance exer...

  19. Einfluss von Konstruktion und Betriebsweise von Trinkwasserinstallationen auf das Wachstum von Mikroorganismen im Trinkwasser

    OpenAIRE

    Astitouh, F. (Fariha)

    2013-01-01

    Das Wachstumsverhalten von Biofilmen und Krankheitserregern im Wasser aus Trinkwasserinstallationen wird durch Temperatur und Stagnation beeinflusst. Die Vermeidung von „lauwarmen“ Temperaturen, die das Legionellenwachstum begünstigt, stellt höhere Anforderungen an Konstruktion und Betriebsweise von Kaltwassersystemen. Diese Arbeit analysiert Konstruktionsprinzipien von Stockwerksinstallationen auf den Einfluss auf das Wachstum von Mikroorganismen im Trinkwasser „Kalt“. Die Versuchsanlage sim...

  20. Vector-host-parasite inter-relationships in leishmaniasis. IV. Electrophoretic studies on proteins of four vertebrate bloods with and without Leishmania infantum or L. major.

    Science.gov (United States)

    Daba, S; Mansour, N S; Youssef, F G; Shanbaky, N M; el Sawaf, B M

    1997-12-01

    Fifty five protein bands with relative mobilities of 8,954 to 245,471 kilo Daltons (kD) were electrophoretically separated from 12 feeding media of blood from 4 natural vertebrate hosts of Phlebotomus langeroni. The feeding media included human, dog (Canis familiaris), rat (Rattus rattus) and turkey (Melagris gallopava) bloods without or with Leishmania infantum or L. major promastigotes. Protein bands were identical among the feeding media of one host's blood but varied in number (24-28 bands) and relative mobilities among the various hosts' blood. Some protein fractions were common among the various hosts blood, others were only present in two or three hosts' blood and some were restricted to one host blood and were unique for each host. This study provides data which may help in understanding why blood from different natural hosts may variably influence the life cycle of Leishmania parasite in the sand fly gut. PMID:9425823

  1. Concentration of total proteins in blood plasma of chickens hatched from irradiated eggs with low dose gamma radiation

    International Nuclear Information System (INIS)

    It is known that low-dose ionising radiation may have stimulating effects on chickens. Low doses may also cause changes in the concentration of blood plasma total proteins, glucose and cholesterol in chickens. This study investigates the effects of low dose gamma-radiation on the concentration of total proteins in the blood plasma of chickens hatched from eggs irradiated with a dose of 0.15 Gy on incubation days 7 and 19. Results were compared with the control group (chickens hatched from non-irradiated eggs). After hatching, all other conditions were the same for both groups. Blood samples were drawn from the heart, and later from the wing vein on days 1, 3, 5, 7,10, 20, 30 and 42. The concentration of total proteins was determined spectrophotometrically using Boehringer Mannheim GmbH optimised kits. The concentration of total proteins in blood plasma in chickens hatched from eggs irradiated with 0.15 Gy on incubation day 7 showed a statistically significant decrease on the sampling day 3 (P less than 0.05) and 7 (P less than 0.01). The concentration of total proteins in blood plasma in chickens hatched from eggs irradiated with 0.15 Gy on incubation day 19 showed a statistically significant increase only on sampling day 1 (P less than 0.05). These results suggest that exposure of eggs to 0.15 Gy of gamma-radiation on the 7th and 19th day of incubation could produce different effects on the protein metabolism in chickens.(author)

  2. Targeted mass spectrometry analysis of the proteins IGF1, IGF2, IBP2, IBP3 and A2GL by blood protein precipitation

    DEFF Research Database (Denmark)

    Such-Sanmartín, Gerard; Bache, Nicolai; Callesen, Anne K;

    2015-01-01

    aggravated when using fast high-throughput methods, which are necessary for analysis of hundreds and thousands of samples in clinical laboratories. The blood proteins IGF1, IGF2, IBP2, IBP3 and A2GL have been proposed as indirect biomarkers for detection of GH administration and as putative biomarkers for...

  3. Langzeitkultur von humanen Langerhanszellen

    OpenAIRE

    Henschke, Cornelia

    2001-01-01

    Die Arbeit beschreibt das phänotypische Verhalten von kultivierten Langerhanszellen, antigenpräsentierenden Zellen der Epidermis, sowie die Art und Weise ihrer Elimination. Hierfür wurden Zellkulturen von Langerhanszellen durch Migration aus normaler menschlicher Haut gewonnen. Die Langerhanszellen durchlaufen dabei die gleiche funktionelle Entwicklung, wie nach Antigenpräsentation in situ. Ziel der Untersuchung war es, die funktionellen und zellulären Eigenschaften und die Elimination von...

  4. Neue Inschriften von Olympia

    OpenAIRE

    Taeuber, Hans; Siewert, Peter

    2013-01-01

    Das Heiligtum von Olympia fasziniert die Menschen von der Antike bis in die Gegenwart. Uber seine Geschichte und den Ablauf der dort abgehaltenen Spiele erhalten wir authentische Informationen von antiken Schriftstellern wie Pausanias, durch Grabungsfunde und -befunde, vor allem aber auch durch die dort gefundenen Inschriften. Die vorliegende Publikation vereint nun die seit 1896 veröffentlichten Texte und ermoglicht somit eine vollständige Ubersicht über die epigraphischen Zeugnisse auf Stei...

  5. Determination of olanzapine in whole blood using simple protein precipitation and liquid chromatography-tandem mass spectrometry

    DEFF Research Database (Denmark)

    Nielsen, Marie Katrine Klose; Johansen, Sys Stybe

    2009-01-01

    A simple, sensitive, and reproducible liquid chromatography-tandem mass spectrometry method has been developed and validated for the quantification of the antipsychotic drug olanzapine in whole blood using dibenzepine as internal standard (IS). After acidic methanol-induced protein precipitation of...

  6. C-reactive protein and white blood cell count do not improve clinical decision-making in acute appendicitis

    DEFF Research Database (Denmark)

    Tind, Sofie; Lassen, Annmarie Touborg; Zimmermann-Nielsen, Erik;

    2015-01-01

    INTRODUCTION: Acute appendicitis (AA) remains a diagnostic challenge as indicated by the high rate of unnecessary surgery. Blood samples, primarily C-reactive protein (CRP) and leucocyte counts, are used as a diagnostic supplement despite their relatively low sensitivities and specificities...... leucocyte counts did not influence clinical decision-making....

  7. The effect of varying protein levels on blood chemistry, food consumption, and behavior of captive seaducks

    Science.gov (United States)

    Wells-Berlin, A. M.; Perry, M.C.; Olsen, G.H.

    2005-01-01

    The Chesapeake Bay is a primary wintering area for scoters and the long-tailed ducks (Clangia hyemalis) that migrate along the Atlantic Flyway. Recently, the Chesapeake Bay had undergone an ecosystem shift and little is known about how this is affecting the seaduck populations. We are determining what are the preferred food sources of the seaducks wintering on the Bay and analyzing the factors influencing prey selection whether it is prey composition, energy assimilated, prey availability, or a combination of any or all of these factors. We have established a captive colony of surf (Melanitta perspicillata) and white-winged scoters (Melanitta fusca) as well as long-tailed ducks at Patuxent Wildlife Research Center to allow us to examine these factors in a more controlled environment. This project contains a multitude of experiments and the resultant data will be compiled into a compartmental model on the feeding ecology of seaducks wintering on the Bay. The first experiment entailed feeding groups of each species (four ducks per pen of equal sex ratio, if possible, and four pens per species) three diets varying in percent protein levels from November to February. Each diet was randomly assigned to each pen and the amount of food consumed was recorded each day. New feed was given when all existing food was consumed. Behavioral trials and blood profiles were completed on all study birds to determine the effects of the varying diets. There were no significant differences in food consumption, blood chemistry, and behavior detected at the 5% level among the diets for all three species of interest. There was a seasonal effect determined based on the food consumption data for white-winged scoters, but not for surf scoters or long-tailed ducks. The blood profiles of the surf scoters were compared to blood profiles of wild surf scoters and a there was no difference detected at the 5% level. As a health check of the ducks an aspergillosis test was run on the blood obtained

  8. Blood profile of proteins and steroid hormones predicts weight change after weight loss with interactions of dietary protein level and glycemic index.

    Directory of Open Access Journals (Sweden)

    Ping Wang

    Full Text Available BACKGROUND: Weight regain after weight loss is common. In the Diogenes dietary intervention study, high protein and low glycemic index (GI diet improved weight maintenance. OBJECTIVE: To identify blood predictors for weight change after weight loss following the dietary intervention within the Diogenes study. DESIGN: Blood samples were collected at baseline and after 8-week low caloric diet-induced weight loss from 48 women who continued to lose weight and 48 women who regained weight during subsequent 6-month dietary intervention period with 4 diets varying in protein and GI levels. Thirty-one proteins and 3 steroid hormones were measured. RESULTS: Angiotensin I converting enzyme (ACE was the most important predictor. Its greater reduction during the 8-week weight loss was related to continued weight loss during the subsequent 6 months, identified by both Logistic Regression and Random Forests analyses. The prediction power of ACE was influenced by immunoproteins, particularly fibrinogen. Leptin, luteinizing hormone and some immunoproteins showed interactions with dietary protein level, while interleukin 8 showed interaction with GI level on the prediction of weight maintenance. A predictor panel of 15 variables enabled an optimal classification by Random Forests with an error rate of 24±1%. A logistic regression model with independent variables from 9 blood analytes had a prediction accuracy of 92%. CONCLUSIONS: A selected panel of blood proteins/steroids can predict the weight change after weight loss. ACE may play an important role in weight maintenance. The interactions of blood factors with dietary components are important for personalized dietary advice after weight loss. REGISTRATION: ClinicalTrials.gov NCT00390637.

  9. Effect of Peumus boldus on the labeling of red blood cells and plasma proteins with Technetium-99m

    International Nuclear Information System (INIS)

    Peumus boldus is used in popular medicine in Brazil. The influence of Peumus boldus on the labeling of red blood cells and plasma proteins with 99mTc was studied. Stannous chloride and 99mTc pertechnetate were incubated with blood and a tincture of Peumus boldus. Aliquots of plasma and blood cells were isolated from the mixture and treated with trichloroacetic acid (TCA). After separation, analysis of the soluble and insoluble fractions showed a rapid uptake of the radioactivity by blood cells in the presence of the drug, whereas there was a slight decrease in the amount of 99mTc radioactivity in the TCA-insoluble fraction of plasma

  10. Effect of Peumus boldus on the labeling of red blood cells and plasma proteins with Technetium-99m

    Energy Technology Data Exchange (ETDEWEB)

    Wancke Reiniger, Ingrid; Fonseca de Oliveira, Joelma; Caldeira-de-Araujo, Adriano [Departamento de Biofisica e Biometria, Instituto de Biologia Roberto Alcantara Gomes, Universidade do Estado do Rio de Janeiro, Rio de Janeiro, RJ (Brazil); Bernardo-Filho, Mario [Instituto Nacional de Cancer, Centro de Pesquisa Basica, Rio de Janeiro, RJ (Brazil)

    1999-08-01

    Peumus boldus is used in popular medicine in Brazil. The influence of Peumus boldus on the labeling of red blood cells and plasma proteins with {sup 99m}Tc was studied. Stannous chloride and {sup 99m}Tc pertechnetate were incubated with blood and a tincture of Peumus boldus. Aliquots of plasma and blood cells were isolated from the mixture and treated with trichloroacetic acid (TCA). After separation, analysis of the soluble and insoluble fractions showed a rapid uptake of the radioactivity by blood cells in the presence of the drug, whereas there was a slight decrease in the amount of {sup 99m}Tc radioactivity in the TCA-insoluble fraction of plasma.

  11. Effect of Peumus boldus on the labeling of red blood cells and plasma proteins with technetium-99m.

    Science.gov (United States)

    Reiniger, I W; de Oliveira, J F; Caldeira-de-Araújo, A; Bernardo-Filho, M

    1999-08-01

    Peumus boldus is used in popular medicine in Brazil. The influence of Peumus boldus on the labeling of red blood cells and plasma proteins with 99mTc was studied. Stannous chloride and 99mTc pertechnetate were incubated with blood and a tincture of Peumus boldus. Aliquots of plasma and blood cells were isolated from the mixture and treated with trichloroacetic acid (TCA). After separation, analysis of the soluble and insoluble fractions showed a rapid uptake of the radioactivity by blood cells in the presence of the drug, whereas there was a slight decrease in the amount of 99mTc radioactivity in the TCA-insoluble fraction of plasma. PMID:10376326

  12. Towards a Proteomic Catalogue and Differential Annotation of Salivary Gland Proteins in Blood Fed Malaria Vector Anopheles culicifacies by Mass Spectrometry.

    Science.gov (United States)

    Rawal, Ritu; Vijay, Sonam; Kadian, Kavita; Singh, Jagbir; Pande, Veena; Sharma, Arun

    2016-01-01

    In order to understand the importance of functional proteins in mosquito behavior, following blood meal, a baseline proteomic dataset is essential for providing insights into the physiology of blood feeding. Therefore, in this study as first step, in solution and 1-D electrophoresis digestion approach combined with tandem mass spectrometry (nano LC-MS/MS) and computational bioinformatics for data mining was used to prepare a baseline proteomic catalogue of salivary gland proteins of sugar fed An. culicifacies mosquitoes. A total of 106 proteins were identified and analyzed by SEQUEST algorithm against mosquito protein database from Uniprot/NCBI. Importantly, D7r1, D7r2, D7r4, salivary apyrase, anti-platelet protein, calreticulin, antigen 5 family proteins were identified and grouped on the basis of biological and functional roles. Secondly, differential protein expression and annotations between salivary glands of sugar fed vs blood fed mosquitoes was analyzed using 2-Delectrophoresis combined with MALDI-TOF mass spectrometry. The alterations in the differential expression of total 38 proteins was observed out of which 29 proteins like beclin-1, phosphorylating proteins, heme oxygenase 1, ferritin, apoptotic proteins, coagulation and immunity like, serine proteases, serpins, c-type lectin and protein in regulation of blood feeding behavior were found to be up regulated while 9 proteins related to blood feeding, juvenile hormone epoxide hydrolase ii, odorant binding proteins and energy metabolic enzymes were found to be down regulated. To our knowledge, this study provides a first time baseline proteomic dataset and functional annotations of An. culicifacies salivary gland proteins that may be involved during the blood feeding. Identification of differential salivary proteins between sugar fed and blood fed mosquitoes and their plausible role may provide insights into the physiological processes associated with feeding behavior and sporozoite transmission during the

  13. Near infrared light induces post-translational modifications of human red blood cell proteins.

    Science.gov (United States)

    Walski, Tomasz; Dyrda, Agnieszka; Dzik, Małgorzata; Chludzińska, Ludmiła; Tomków, Tomasz; Mehl, Joanna; Detyna, Jerzy; Gałecka, Katarzyna; Witkiewicz, Wojciech; Komorowska, Małgorzata

    2015-11-01

    There is a growing body of evidence that near infrared (NIR) light exerts beneficial effects on cells. Its usefulness in the treatment of cancer, acute brain injuries, strokes and neurodegenerative disorders has been proposed. The mechanism of the NIR action is probably of photochemical nature, however it is not fully understood. Here, using a relatively simple biological model, human red blood cells (RBCs), and a polychromatic non-polarized light source, we investigate the impact of NIR radiation on the oxygen carrier, hemoglobin (Hb), and anion exchanger (AE1, Band 3). The exposure of intact RBCs to NIR light causes quaternary transitions in Hb, dehydration of proteins and decreases the amount of physiologically inactive methemoglobin, as detected by Raman spectroscopy. These effects are accompanied by a lowering of the intracellular pH (pHi) and changes in the cell membrane topography, as documented by atomic force microscopy (AFM). All those changes are in line with our previous studies where alterations of the membrane fluidity and membrane potential were attributed to NIR action on RBCs. The rate of the above listed changes depends strictly on the dose of NIR light that the cells receive, nonetheless it should not be considered as a thermal effect. PMID:26329012

  14. The Pf332 gene codes for a megadalton protein of Plasmodium falciparum asexual blood stages

    Directory of Open Access Journals (Sweden)

    Denise Mattei

    1992-01-01

    Full Text Available We characterized the Plasmodium falciparum antigen 332 (Ag332 which is specifically expressed during the asexual intraerythrocytic cycle of the parasite. The corresponding Pf332 gene has been located in the subtelomeric region of chromosome 11. Furthermore, it is present in all strais so far analyzed and shows marked restriction length fragment polymorphism. Partial sequence and restriction endonuclease digestion of cloned fragments revealed that the Pf332 gene is composed of highly degenerated repeats rich is glutamic acid. Mung been nuclease digestion and Northern blot analysis suggested that Pf332 gene codes for a protein of about 700 kDa. These data were further confirmed by Western blot and immunoprecipitation of parasites extracts with an antiserum raised against a recombinant clone expressing part of the Ag332. Confocal immunofluorescence showed that Ag332 is translocated from the parasite to the surface of infected red blood cells within vesicle-like structures. In addition, Ag332 was detected on the surface of monkey erythrocytes infected with Plasmodium falciparum.

  15. [Genetic polymorphism of blood protein of six Passeriformes species in Zhalong National Nature Reserve].

    Science.gov (United States)

    Xu, Xing-Jun; Lü, Jian-Wei; Xuan, Li-Fang; Zhang, Dong-Yue; Zhou, Shuang-Tao; Zhao, Kan; Wang, Bin; Shao, Shu-Li

    2009-06-01

    The polymorphism of hemoglobin (Hb), serum albumin (Alb), trandferring (Tf) and adenosin deaminase (Ada) in the blood of Emberiza elegans, Phylloscopus inornatus, E. aureola, Lanius tigrinus, Passer montanus, and E. spodocephala in Zhalong National Nature Reserve were studied by SDS-polyacrylamide-gel electrophoresis. All the protein loci of the six Passeriformes species exhibited polymorphism, and Ada locus had a relatively high heterozygosity. The analysis of average heterozygosity demonstrated that P. inornatus had a higher population genetic variance, while E. spodocephala was relatively stable. The six species could be classified into two clusters. E. elegans, E. aureola, E. spodocephala and P. inornatus were clustered into one group, while P. montanus and L. tigrinus were clustered into another group. This clustering was consistent with the actual taxonomic status of the six species. The higher thermoregulation index and predation pressure of the study area did not lead to the substantial variation of hereditary constitution of the six species, possibly due to the gene intercommunion between the six species and the outside of the study area, which decreased the variance and differentiation of the population genetics of the six species. PMID:19795660

  16. Effects of previous protein intake on rectal temperature, blood glucose, plasma thyroid hormone and minerals by laying hens during a forced molt

    International Nuclear Information System (INIS)

    The effects of forced molting on blood glucose, rectal temperature, plasma T4, T3 and minerals were studied in hens previously fed rations with different protein contents (14, 17 and 20% crude protein). Blood samples were obtained from brachial veins for blood glucose, T4 and T3 were measured by radioimmunoassay, and plasma minerals were determined by atomic absorption spectroscopy. Blood glucose and rectal temperature were reduced during fasting regardless of previous protein intake. Pre molting T4 plasma level was higher in laying hens fed higher protein ration, but feed deprivation reduced T4 and T3 concentrations irrespective of protein intake, except T4 level for 14% crude protein fed birds that increased during fasting. The data obtained in this experiment suggest that previous protein intake does not interfere with the metabolic changes during forced molt. (author). 19 refs, 1 fig, 4 tabs

  17. Statistische Besonderheiten von Finanzmarktdaten

    OpenAIRE

    Krämer, Walter

    2000-01-01

    Finanzmarktdaten wie Zinsen, Aktien- oder Wechselkurse und andere spekulative Preise setzen sich durch verschiedene Besonderheiten von sonstigen ökonomischen Zeitreihendaten ab. Dieser Artikel untersucht die Konsequenzen dieser Besonderheiten für die rationale Bewertung von Finanzinstrumenten und für verschiedene, in finanzwirtschaftlichen Anwendungen angewandte statistische Schätzungen und Tests.

  18. Kointegration von Aktienkursen

    OpenAIRE

    Krämer, Walter

    1997-01-01

    Die vorliegende Arbeit untersucht das gemeinsame Zeitreihenverhalten von spekulativen Preisen, speziell von Aktienkursen. Anhand ausgewählter deutscher Dividendenwerte wird gezeigt, daß dieses gemeinsame Zeitreihenverhalten zu den Markt schlagenden Anlagestrategien führen kann und in diesem Sinn mit effizienten Märkten nicht verträglich ist.

  19. Lebenszyklusbasierte Nachhaltigkeitsanalyse von Technologien

    OpenAIRE

    Lehmann, Annekatrin

    2013-01-01

    ZIEL: Eine Bewertung von Technologien unter Nachhaltigkeitsgesichtspunkten erfordert die Betrachtung verschiedener Dimensionen und verschiedener Betrachtungsebenen. Das Ziel dieser Arbeit ist es, eine Antwort auf die Forschungsfrage zu geben, wie eine lebenszyklusbasierte, produktbezogene und vergleichende Nachhaltigkeitsanalyse von Technologien umgesetzt werden kann. Die Erörterung erfolgt sowohl theoretisch als auch praktisch am Beispiel einer Fallstudie zur Analyse alternativer Technologie...

  20. Mikrobieller Abbau von Iminodisuccinat

    OpenAIRE

    Cokesa, Zeljko

    2004-01-01

    Iminodisuccinat (IDS) ist ein neuer synthetisch hergestellter Komplexbildner mit dem Potenzial den meistbenutzten aber schwer abbaubaren Komplexbildner Ethylendiamin-tetraacetat (EDTA) teilweise zu ersetzen. Von einem EDTA-Substitut wird neben guten Komplexierungseigenschaften insbesondere eine gute biologische Abbaubarkeit verlangt. In diesem Zusammenhang wurde die Abbaubarkeit von IDS bewertet, die initialen Abbauwege aufgeklärt und die in den Abbau involvierten Gene charakterisiert. Sta...

  1. Einfluss von Titan auf den Entwurf von Unterwasserfahrzeugen

    OpenAIRE

    Malletschek, Andreas

    2011-01-01

    Die Dissertation zum „Einfluss von Titan auf den Entwurf von Unterwasserfahrzeugen“ beschäftigt sich sowohl mit der Erarbeitung der werkstoffseitigen Grundlagen für die allgemeine Integration von Titan in der Meerestechnik als auch mit der konkreten Analyse und Bewertung der Anwendung von Titan in verschiedenen schiffstechnischen Systeme eines Unterwasserfahrzeugs.

  2. Production of soluble recombinant proteins with Kell, Duffy and Lutheran blood group antigen activity, and their use in screening human sera for Kell, Duffy and Lutheran antibodies.

    Science.gov (United States)

    Ridgwell, K; Dixey, J; Scott, M L

    2007-10-01

    The aim of this study was to show that soluble recombinant (sr) proteins can mimic blood group antigens and be used to screen human sera for blood-group-specific antibodies. The blood of all pregnant women and pretransfusion patients should be screened for blood-group-specific antibodies to identify and monitor pregnancies at risk of haemolytic disease of the foetus and newborn (HDFN), and to prevent haemolytic transfusion reactions. Current antibody screening and identification methods use human red blood cell panels, which can complicate antibody identification if more than one antibody specificity is present. COS-7 cells were transfected to produce sr forms of the extracellular domains of the red blood cell membrane proteins that express Kell, Duffy or Lutheran blood group antigens. These sr proteins were used to screen for and identify anti-Kell, anti-Duffy or anti-Lutheran blood-group-specific allo-antibodies in human sera by haemagglutination inhibition and in solid-phase enzyme-linked immunosorbent assays (ELISAs). There is a positive correlation (correlation coefficient 0.605, P value 0.002) between antibody titre by standard indirect antiglobulin test (IAT) and signal intensity in the ELISA test. This work shows that sr proteins can mimic blood group antigens and react with human allogeneic antibodies, and that such proteins could be used to develop solid-phase, high-throughput blood group antibody screening and identification platforms. PMID:17725551

  3. Regulation des Na+-D-Glucose Kotranporters SGLT1 im Dünndarm nach bariatrischen Operation und durch von Protein RS1 (RSC1A1) abgeleitete Peptide

    OpenAIRE

    Rikkala, Prashanth Reddy

    2016-01-01

    Bariatrische Operationen repräsentieren die Behandlung erster Wahl bei krankhafter Fettleibigkeit, resultierend in Gewichtsverlust und verbesserter Diabetes-Kontrolle. Der positive Effekt bariatrischer Operationen auf den Typ-2 Diabetes ist unklar. Erhöhte Sekretion von Insulin, welches das Enterohormon „Glucagon-like-peptide 1“ (GLP-1) reguliert, wurde beobachtet bei Ratten mit experimentellem Typ 2-ähnlichem Diabetes nach duodenalem-jejunalem Bypass (DJB) und ilealer Transposition (IT). Der...

  4. Blood levels of critical amino acids in very low birthweight infants on a high human milk protein intake.

    Science.gov (United States)

    Lindblad, B S; Hagelberg, S; Lundsjö, A

    1982-01-01

    A method for a semi-industrial production of human milk subfractions (human milk protein and human milk fat isolates) is described. Four very low birthweight (VLBW) newborn were given a human milk protein isolate added to the mother's own fresh expressed milk in addition to sodium chloride up to 20 mEkv/liter. Growth followed the intrauterine growth curve. Urea levels did not increase in spite of providing a double-normal protein intake. There was no metabolic acidosis and the blood levels of free amino acids determined with a micro-method did not exceed those seen after a normal meal. The concentrated human milk protein product showed a considerable specific sIgA activity against E. coli 0-antigen. It seems possible to use similar "lacto-engineering"-techniques in order to satisfy the increased protein requirements of the VLBW infant, while providing the caloric requirements, without causing any visible disturbance of blood-homeostasis of urea, amino acids or base excess. The method could provide knowledge about the "human milk protein requirements" and a controlled study has been started. PMID:6961736

  5. Aufbau von Navigationshilfen durch Analyse von Suchanfragen

    OpenAIRE

    Höchstötter, Nadine

    2007-01-01

    Nadine Höchstötter, Karlsruhe, berichtete über den letzten Stand ihrer Forschungen zum Suchverhalten in Suchmaschinen. Anhand von Datensätzen in Lycos lassen sich Muster von Suchanfragen ermitteln, aus denen Trends abgeleitet werden können. Es kann dabei zwischen vier grundsätzlichen Klassen unterschieden werden: „Eintagsfliegen“ treten im Erhebungszeitraum nur einmal auf, „Dauerbrenner“ hingegen während der Erhebungsperiode nahezu in jedem definierten Zeitintervall. Kleinere Ausschläge, bei ...

  6. Effect of an extract of Artemisia vulgaris L. (Mugwort) on the in vitro labeling of red blood cells and plasma proteins with technetium-99m

    International Nuclear Information System (INIS)

    The aim of this work was to evaluate the effect of an extract of the Artemisia vulgaris L. (mugwort) on the labeling of blood constituents with technetium-99m (99mTc). Blood samples from Wistar rats were incubated with a mugwort extract and the radiolabeling of blood constituents was carried out. Plasma and blood cells were separated by centrifugation. Aliquots of plasma and blood cells were also precipitated with trichloroacetic acid and centrifuged to isolate soluble and insoluble fractions of plasma and blood cells. Radioactivity in each fraction was counted and the percentages of radioactivity (%ATI) was calculated. Mugwort extract decreased significantly (p<0.05) the %ATI on the blood compartments and on the blood cells proteins (insoluble fraction). The analysis of the results indicates that the extract could have substances that could interfere on the transport of stannous through the erythrocyte membrane altering the labeling of blood cells with 99mTc. (author)

  7. Enhanced neutralization potency of botulinum neurotoxin antibodies using a red blood cell-targeting fusion protein.

    Directory of Open Access Journals (Sweden)

    Sharad P Adekar

    Full Text Available Botulinum neurotoxin (BoNT potently inhibits cholinergic signaling at the neuromuscular junction. The ideal countermeasures for BoNT exposure are monoclonal antibodies or BoNT antisera, which form BoNT-containing immune complexes that are rapidly cleared from the general circulation. Clearance of opsonized toxins may involve complement receptor-mediated immunoadherence to red blood cells (RBC in primates or to platelets in rodents. Methods of enhancing immunoadherence of BoNT-specific antibodies may increase their potency in vivo. We designed a novel fusion protein (FP to link biotinylated molecules to glycophorin A (GPA on the RBC surface. The FP consists of an scFv specific for murine GPA fused to streptavidin. FP:mAb:BoNT complexes bound specifically to the RBC surface in vitro. In a mouse model of BoNT neutralization, the FP increased the potency of single and double antibody combinations in BoNT neutralization. A combination of two antibodies with the FP gave complete neutralization of 5,000 LD50 BoNT in mice. Neutralization in vivo was dependent on biotinylation of both antibodies and correlated with a reduction of plasma BoNT levels. In a post-exposure model of intoxication, FP:mAb complexes gave complete protection from a lethal BoNT/A1 dose when administered within 2 hours of toxin exposure. In a pre-exposure prophylaxis model, mice were fully protected for 72 hours following administration of the FP:mAb complex. These results demonstrate that RBC-targeted immunoadherence through the FP is a potent enhancer of BoNT neutralization by antibodies in vivo.

  8. Subcompartmentalisation of proteins in the rhoptries correlates with ordered events of erythrocyte invasion by the blood stage malaria parasite.

    Directory of Open Access Journals (Sweden)

    Elizabeth S Zuccala

    Full Text Available Host cell infection by apicomplexan parasites plays an essential role in lifecycle progression for these obligate intracellular pathogens. For most species, including the etiological agents of malaria and toxoplasmosis, infection requires active host-cell invasion dependent on formation of a tight junction - the organising interface between parasite and host cell during entry. Formation of this structure is not, however, shared across all Apicomplexa or indeed all parasite lifecycle stages. Here, using an in silico integrative genomic search and endogenous gene-tagging strategy, we sought to characterise proteins that function specifically during junction-dependent invasion, a class of proteins we term invasins to distinguish them from adhesins that function in species specific host-cell recognition. High-definition imaging of tagged Plasmodium falciparum invasins localised proteins to multiple cellular compartments of the blood stage merozoite. This includes several that localise to distinct subcompartments within the rhoptries. While originating from the same organelle, however, each has very different dynamics during invasion. Apical Sushi Protein and Rhoptry Neck protein 2 release early, following the junction, whilst a novel rhoptry protein PFF0645c releases only after invasion is complete. This supports the idea that organisation of proteins within a secretory organelle determines the order and destination of protein secretion and provides a localisation-based classification strategy for predicting invasin function during apicomplexan parasite invasion.

  9. Identification of an additional class of C3-binding membrane proteins of human peripheral blood leukocytes and cell lines.

    OpenAIRE

    Cole, J L; Housley, G A; Dykman, T R; MacDermott, R P; Atkinson, J P

    1985-01-01

    Proteins binding the third component of complement (C3) were isolated by affinity chromatography from surface-labeled solubilized membranes of human peripheral blood cells and cell lines. The isolated molecules were subjected to NaDodSO4/PAGE, and autoradiographs of these gels indicated that C3-binding proteins could be divided into three groups based on Mr: (i) gp200, an approximately 200,000 Mr molecule previously identified as the C3b/C4b receptor or CR1; (ii) gp140, an approximately 140,0...

  10. Protein-Based Blood Substitutes: Recent Attempts at Controlling Pro-Oxidant Reactivity with and Beyond Hemoglobin

    Directory of Open Access Journals (Sweden)

    Radu Silaghi-Dumitrescu

    2013-07-01

    Full Text Available Reviewed here are recent attempts to produce protein-based artificial oxygen carriers (“blood substitutes”. Most of these involve chemical or physical modifications on hemoglobin, although a recent line of research using hemerythrin instead of hemoglobin is also described. The focus is set on the extent to which these modifications alter the redox reactivity of the proteins, and on ways in which this can be done systematically and purposefully, within the framework of a working hypothesis where redox side-reactions hold an important role in the physiological outcome of experimental transfusions with artificial oxygen carriers.

  11. Gruppen von quasi-Automorphismen

    OpenAIRE

    Lehnert, Jörg

    2008-01-01

    Die vorliegende Arbeit beschäftigt sich mit Gruppen von quasi-Automorphismen von Graphen, genauer gesagt, von gefärbten Graphen. Ein gefärbter Graph ist ein Graph, dessen Kantenmenge in eine disjunkte Vereinigung von Mengen von Kanten einer bestimmten Farbe zerlegt ist. Ein Automorphismus eines solchen Graphen muss insbesondere die Farben der Kanten respektieren. Ein quasi-Automorphismus eines solchen Graphen ist eine Bijektion der Eckenmenge auf sich selbst, die nur endlich oft die Autmomorp...

  12. Serum, plasma, and dried blood spot high sensitivity C-reactive protein enzyme immunoassay for population research

    OpenAIRE

    Brindle, Eleanor; FUJITA, MASAKO; Shofer, Jane; O’Connor, Kathleen A.

    2010-01-01

    C-reactive protein (CRP) is used as a biomarker of morbidity and mortality risk in studies of population health, and is essential to interpretation of several micronutrient biomarkers. There is thus need for a robust high sensitivity CRP (hsCRP) measurement method for large-scale, non-clinical studies. We developed an efficient, inexpensive assay suitable for quantifying CRP across the physiological range using any blood specimen type. The ELISA uses readily available monoclonal antibodies to...

  13. Correlation of serum C-reactive protein, white blood count and neutrophil percentage with histopathology findings in acute appendicitis

    OpenAIRE

    Xharra Shefki; Gashi-Luci Lumturije; Xharra Kumrije; Veselaj Fahredin; Bicaj Besnik; Sada Fatos; Krasniqi Avdyl

    2012-01-01

    Abstract Background Acute appendicitis is one of the most common surgical emergencies. Accurate diagnosis of acute appendicitis is based on careful history, physical examination, laboratory and imaging investigation. The aim of the study is to analyze the role of C-reactive protein (CRP), white blood count (WBC) and Neutrophil percentage (NP) in improving the accuracy of diagnosis of acute appendicitis and to compare it with the intraoperative assessment and histopathology findings. Materials...

  14. Blood-brain barrier delivery of protein and non-viral gene therapeutics with molecular Trojan horses

    OpenAIRE

    Pardridge, William M

    2007-01-01

    The products of biotechnology, recombinant proteins, monoclonal antibodies, antisense, RNA interference, or non-viral gene transfer, cannot be developed as pharmaceuticals for the brain, unless these molecules are re-formulated to enable transport across the blood-brain barrier (BBB). Large molecule drugs, and plasmid DNA, can be delivered across the BBB with receptor-specific molecular Trojan horses. Trojan horse BBB delivery systems, coupled with one of 3 different technology platforms (fus...

  15. Blood flow restriction exercise stimulates mTORC1 signaling and muscle protein synthesis in older men

    OpenAIRE

    Fry, Christopher S.; Glynn, Erin L.; Drummond, Micah J.; Timmerman, Kyle L.; Fujita, Satoshi; Abe, Takashi; Dhanani, Shaheen; Volpi, Elena; Rasmussen, Blake B.

    2010-01-01

    The loss of skeletal muscle mass during aging, sarcopenia, increases the risk for falls and dependence. Resistance exercise (RE) is an effective rehabilitation technique that can improve muscle mass and strength; however, older individuals are resistant to the stimulation of muscle protein synthesis (MPS) with traditional high-intensity RE. Recently, a novel rehabilitation exercise method, low-intensity RE, combined with blood flow restriction (BFR), has been shown to stimulate mammalian targ...

  16. Extensive surface protein profiles of extracellular vesicles from cancer cells may provide diagnostic signatures from blood samples

    OpenAIRE

    Belov, Larissa; Matic, Kieran J.; Hallal, Susannah; Mulligan, Stephen P.; Best, O. Giles; Christopherson, Richard I

    2016-01-01

    Extracellular vesicles (EV) are membranous particles (30–1,000 nm in diameter) secreted by cells. Important biological functions have been attributed to 2 subsets of EV, the exosomes (bud from endosomal membranes) and the microvesicles (MV; bud from plasma membranes). Since both types of particles contain surface proteins derived from their cell of origin, their detection in blood may enable diagnosis and prognosis of disease. We have used an antibody microarray (DotScan) to compare the surfa...

  17. Die Messung von Vernetzung

    OpenAIRE

    Kreische, Joachim

    2006-01-01

    Die Nutzungsanalyse der von Bibliotheken angebotenen Datenbanken und Volltextangebote ist bisher nicht nur stark von den Datenlieferungen der Anbieter abhängig, sondern auch sehr statisch. Mit der Einführung von Linking Resolvern, die über Statistiktools verfügen, verbessern sich die Möglichkeiten der Nutzungsanalyse deutlich. Am Beispiel der Einführung des Linking Resolvers SFX an der ULB Düsseldorf sollen verschiedene Analysemöglichkeiten vorgestellt werden. Insbesondere die über den Linkin...

  18. Chemical composition and biological value of spray dried porcine blood by-products and bone protein hydrolysate for young chickens.

    Science.gov (United States)

    Jamroz, D; Wiliczkiewicz, A; Orda, J; Skorupińska, J; Słupczyńska, M; Kuryszko, J

    2011-10-01

    The chemical composition of spray dried porcine blood by-products is characterised by wide variation in crude protein contents. In spray dried porcine blood plasma (SDBP) it varied between 670-780 g/kg, in spray dried blood cells (SDBC) between 830-930 g/kg, and in bone protein hydrolysate (BPH) in a range of 740-780 g/kg. Compared with fish meal, these feeds are poor in Met and Lys. Moreover, in BPH deep deficits of Met, Cys, Thr and other amino acids were found. The experiment comprised 7 dietary treatments: SDBP, SDBC, and BPH, each at an inclusion rate of 20 or 40 g/kg diet, plus a control. The addition of 20 or 40 g/kg of the analysed meals into feeds for very young chickens (1-28 d post hatch) significantly decreased the body weight (BW) of birds. Only the treatments with 40 g/kg of SDBP and SDBC showed no significant difference in BW as compared with the control. There were no significant differences between treatments and type of meal for feed intake, haematocrit and haemoglobin concentrations in blood. Addition of bone protein and blood cell meals to feed decreased the IgG concentration in blood and caused shortening of the femur and tibia bones. However, changes in the mineral composition of bones were not significantly affected by the type of meal used. The blood by-products, which are rich in microelements, improved retention of Ca and Cu only. In comparison to control chickens, significantly better accretion of these minerals was found in treatments containing 20 g/kg of SDBP or 40 g/kg of SDBC. Great variability in apparent ileal amino acid digestibility in chickens was determined. In this respect, some significant differences related to the type of meal fed were confirmed for Asp, Pro, Val, Tyr and His. In general, the apparent ileal digestibility of amino acids was about 2-3 percentage units better in chickens fed on diets containing the animal by products than in control birds. PMID:22029787

  19. Effect of a high-protein diet on maintenance of blood pressure levels achieved after initial weight loss

    DEFF Research Database (Denmark)

    Engberink, M F; Geleijnse, J M; Bakker, S J L;

    2015-01-01

    Randomized trials have shown significant blood pressure (BP) reductions after increased protein compared with carbohydrate intake, but the effect on BP maintenance after initial weight loss is unclear. We examined the effect of a high-protein diet on the maintenance of reduced BP after weight loss...... in 420 overweight adults from the Diet, Obesity and Genes study. After an 8-week weight-loss period (>8% BW), subjects (42±6 years) were randomized to either a high-protein diet (23-28 en% protein) or a lower-protein control diet (10-15 en% protein) for 26 weeks. BMI after weight loss was 30.3±4.3 kg...... m(-2), BP was 118/73 mm Hg and 28 subjects (6.5%) used antihypertensive agents. Systolic BP during 26 weeks of weight maintenance dietary intervention increased in both treatment groups, but it was 2.2 mm Hg less (95% CI: -4.6 to 0.2 mm Hg, P=0.08) in the high-protein group than in the lower...

  20. Ellis Von Creveld Syndrome

    Directory of Open Access Journals (Sweden)

    Afshar H

    1999-01-01

    Full Text Available One patient with Ellis Von Creveld syndrome contains: dwarfism, congenital heart"ndisease, ectodermal dysplasia, polyductyly, an abnormally wide labial frenum and maxillary"nmolars with single root.

  1. Carl von Clausewitz

    DEFF Research Database (Denmark)

    Højrup, Thomas

    2009-01-01

    Forskningsbaseret præsentation af Carl von Clausewitz' krigsteori og dens betydning for statsbegrebet og udviklingen af teori om statsdannelse og statssystem. Krigsbegrebets betydning for politikbegrebet ekspliciteres, og de indre relationer imellem de to begreber demonstreres med konkrete...

  2. Produktentwicklung von Druckgussteilen

    OpenAIRE

    Schübel, Christine

    2012-01-01

    Die Produktentwicklung von Druckgussteilen kann flexibel, wirtschaftlich und schnell durch die Herstellung von RP-Unikaten und Metallabgüssen aus Keramikschalen realisiert werden. Dieser Weg eignet sich vor allem für komplexe Bauteile und macht eine Produkterprobung mit qualitativ deutlich verbesserter Aussagekraft trotz reduzierter Entwicklungskosten möglich. In Abgrenzung zum Sandguss, muss die Konstruktion der Bauteile nicht verändert werden. 
Durch eine gezielte Wärmebehandlung wird die V...

  3. Lars von Triers film

    DEFF Research Database (Denmark)

    Nielsen, Lisbeth Overgaard

    2007-01-01

    Afhandlingen undersøger Lars von Triers filmæstetik, som den kommer til udtryk i spillefilmene fra perioden 1984-2007. Afhandlingen analyserer de enkelte films stil, virkningsstrategi og betydningsdannelse.......Afhandlingen undersøger Lars von Triers filmæstetik, som den kommer til udtryk i spillefilmene fra perioden 1984-2007. Afhandlingen analyserer de enkelte films stil, virkningsstrategi og betydningsdannelse....

  4. Standardization for cortisol determination in human blood by competitive protein-binding

    International Nuclear Information System (INIS)

    Standardization for determination of cortisol from human plasma (17-hydroxycorticosteroids) using competitive protein-binding method is presented. Activated carbon coated with dextrans is used for separation of the hormone-protein complexe and hormone labelled free

  5. Changing optical properties of blood serum proteins in case of oncological diseases

    Science.gov (United States)

    Petrova, G. P.; Petrusevich, Yu. M.; Boiko, A. V.; Ivanov, A. V.; Papish, E. A.; Khlapov, V. P.; Fedorova, K. V.

    2007-03-01

    Molecular methods of diagnostics of widespread diseases including oncological pathology on the base static and dynamic laser light scattering in serum blood solution are testified. Rayleigh - Debye laser light scattering method are used to measure molecular parameters of blood serum. Dynamical parameters of macromolecules can be measured by photon correlation spectroscopy method. We obtained that the parameter of intermolecular interaction B for serum blood solution of oncological patients is considerably less then B for serum blood solution of healthy persons and in a number of cases has even a negative value. The effective mass of scattering particles in serum water solutions for samples of oncology diseases patients increase in comparison to control samples. As follows from our experimental results, there is the difference between dynamic molecular parameters for control samples and ones with oncological pathology.

  6. Associations Between Diabetic Retinopathy and Plasma Levels of High-sensitive C-reactive Protein or Von Willebrand Factor in Long-term Type 1 Diabetic Patients

    DEFF Research Database (Denmark)

    Laursen, Jonas Vejvad Nørskov; Hoffmann, Stine Skovbo; Green, Anders; Nybo, Mads; Sjølie, Anne Katrin; Grauslund, Jakob

    2013-01-01

    population-based cohort from Fyn County, Denmark. Plasma levels of hs-CRP and von Willebrand factor antigen were measured and related to the level of diabetic retinopathy (DR) as evaluated by dilated nine-field 45 degree monoscopic fundus photos captured by Topcon TRC-NWS6 and graded according to the Early...... Treatment Diabetic Retinopathy Study (ETDRS) adaptation of the modified Airlie House classification of DR. Results: Median age and duration of diabetes were 58.7 and 43 years, respectively. Median levels (10th-90th percentile) of hs-CRP and von Willebrand factor antigen were 1.31 mg/l (0.37-13.3 mg/l) and 1.......27 IU/ml (0.79-2.07 IU/ml), respectively. No or minimal DR (ETDRS-levels 10-20) was found in 16.4%, mild DR (ETDRS-level 35) in 19.4%, moderate DR (ETDRS-levels 43-47) in 11.0%, and 53.2% had proliferative diabetic retinopathy (PDR) corresponding to ETDRS-level 60 or more. In an age- and sex...

  7. Homocysteine induces production of monocyte chemoattractant protein-1 and interleukin-8 in cultured human whole blood

    Institute of Scientific and Technical Information of China (English)

    Xiao-kunZENG; DanielGREMICK; XianWANG

    2004-01-01

    AIM: To investigate whether increased plasma L-homocysteine (Hcy) level could promote monocyte chemoattract antprotein-1 (MCP-1) and interleukin-8 (IL-8) in cultured whole blood. METHODS: Human whole blood or differenttype of peripheral blood cells from health volunteers were incubated with Hcy and/or the inhibitors. MCP-1 and IL-8 level were measured by ELISA assay. RESULTS: Hcy 10-1000 μmol/L induced production of MCP-1and IL-8 in cultured human whole blood (P<0.05). The major cellular source of these chemokines comed from monocytes. Meanwhile,Hcy also promoted the upregulation of MPO level even at the 10 μmol/L in the cultured whole blood.The intracellular ROS, particular the OH radicals, play extremely important role in the Hcy-induced MCP-1 and IL-8 production. CONCLUSION: Increased Hcy level in plasma (hyperhomocysteinemia) induced MCP-1 and IL-8secretion in cultured human whole blood, especially in monocytes via oxidative stress mechanism,

  8. Revealing the role of oxidation state in interaction between nitro/amino-derived particulate matter and blood proteins

    Science.gov (United States)

    Liu, Zhen; Li, Ping; Bian, Weiwei; Yu, Jingkai; Zhan, Jinhua

    2016-05-01

    Surface oxidation states of ultrafine particulate matter can influence the proinflammatory responses and reactive oxygen species levels in tissue. Surface active species of vehicle-emission soot can serve as electron transfer-mediators in mitochondrion. Revealing the role of surface oxidation state in particles-proteins interaction will promote the understanding on metabolism and toxicity. Here, the surface oxidation state was modeled by nitro/amino ligands on nanoparticles, the interaction with blood proteins were evaluated by capillary electrophoresis quantitatively. The nitro shown larger affinity than amino. On the other hand, the affinity to hemoglobin is 103 times larger than that to BSA. Further, molecular docking indicated the difference of binding intensity were mainly determined by hydrophobic forces and hydrogen bonds. These will deepen the quantitative understanding of protein-nanoparticles interaction from the perspective of surface chemical state.

  9. Studies on Some Biophysical Properties of the Serum Protein of Mice blood exposed to an electric field

    International Nuclear Information System (INIS)

    As an indication of the effect of the electric field on each of the dielectric properties and the molecular structure of the serum protein of the mice blood, an electric field of a 6 kv/m strength and 50 Hz frequency was directed to three groups of mice for exposure periods 30, 45 and 60 days respectively, and investigated directly. Another group was exposed to also 60 days, but investigated after 30 days from switching off the electric field for delayed effect studies. The molecular structure of the serum protein was studied by measuring each of the dielectric relaxation and the electric conductivity in the frequency range 0.15 MHz at 4 ± 0.5 degree C and the dielectric increment (Δ), relaxation time (τ) and average molecular radii (τ) were calculated for all groups. The absorption spectra of the extracted protein were also measured in the wavelength range 200 600 nm. Moreover, electrophoresis of enzymes B-esterase, lactate and Malate dehydrogenase extracted from the blood serum of exposed mice were taken by using the gel electrophoresis technique. The results indicated that exposure of the animals to 50 H, 6 kv/m electric field resulted in the decrease of serum protein permittivity values and increase its conductivity a fact that indicates pronounced changes in the molecular structure of total serum protein the exposed mice. In addition, the intensity of the absorption spectral bands of serum protein of exposed mice were found to decrease relative to unexposed mice. Also the enzymes B-esterase and lactate dehydrogenase were slightly affected by exposing to the electric field whereas their number of bands and their intensities changed relative to the unexposed mice but the malate dehydrogenase was not affected

  10. Photophysikalische Charakterisierung von Fluoreszenzmarkern insbesondere von Fluoreszenzproteinen

    OpenAIRE

    Auerbach, Dagmar

    2015-01-01

    Die komplette Aufklärung der Vorgänge in Zellen ist von besonderer Bedeutung, da nur durch die gesamte Kenntnis dieser Mechanismen ein Nutzen im Bereich des Wirkstoffdesigns und des Wirkstofftransportes möglich ist. Für weitere Erkenntnisse im Bereich der Fluoreszenzmarker, und im Speziellen der Fluoreszenzproteine, werden in dieser Arbeit Cumarin-basierte Fluoreszenzmarker an Aminosäuren und Peptiden sowie Fluoreszenzproteine charakterisiert. Cumarin-basierte Farbstoffe sind extrinsische Mar...

  11. The effects of protein, amino acid, and dietary electrolyte balance on broiler chicken performance and blood parameters under heat stress

    Directory of Open Access Journals (Sweden)

    Ali Asghar Saki

    2016-08-01

    Full Text Available The effect of crude protein (CP, amino acid (AA, and dietary electrolyte balance (DEB were evaluated on blood parameters, carcass traits, and broiler performance under heat stress (29-34°C. A total of 540 male chickens (Ross 308 were allocated to 12 diets with factorial arrangement 2 × 2 × 3, using a completely randomized design with three replicates of 15 chickens in grower (13 to 26 days and finisher (27 to 42 days periods. and 120, 220, and 320 mEq kg-1 DEB. The level of 21% CP increased body weight gain (BWG and decreased feed conversion ratio (FCR at grower period (p < 0.05. In contrast, 20% CP level decreased BWG and increased FCR at finisher period (p < 0.05. Further, 20% CP level reduced blood sodium and blood electrolyte balance (p < 0.05. The highest blood electrolyte balance was achieved by DEB 320 mEq kg-1 diet (p < 0.05. Broiler response to DEB in heat stress depended on the age of bird, length of exposure to high temperature and CP level of the diet. Under heat stress (29-34°C, the 21% CP level at grower period and 17% CP level at finisher period improved broiler BWG and FCR.

  12. The Expression Level and Clinical Significance of MMP-7 Protein 
in Peripheral Blood in the Patients with Lung Cancer

    OpenAIRE

    SUN, XIAOLIANG; Ting XIAO; Yang, Lei; Gao, Yanning; Guiyu CHENG; Kelin SUN

    2012-01-01

    Background and objective Matrix metalloproteinase 7 (MMP-7), also known as matrilysin, is a member of the MMP family. The objectives of this study were to test MMP-7 protein levels in the peripheral blood of lung cancer patients and healthy control subjects and to determine their corresponding clinical significance. Methods Peripheral blood samples were obtained from 114 lung cancer patients and 100 healthy control subjects. MMP-7 protein levels in the plasma were measured by enzyme-linked im...

  13. In vitro Selektion von Ribozymen mit Proteaseaktivität

    OpenAIRE

    Pfander, Stephanie

    2008-01-01

    Ribonukleinsäuren (RNA) gehören zu den faszinierendsten und spannendsten Biopolymeren der aktuellen Forschung. Ihre mannigfaltigen Funktionen sind für eine Vielzahl von biochemischen Prozessen von essentieller Bedeutung. Zu den bekanntesten Aufgaben der RNA gehört die Umsetzung genetischer Information in Proteine. Dabei ist RNA neben der Eigenschaft als Informationsträger auch aktiver Katalysator für die Knüpfung der Peptidbindung im Ribosom. Die funktionelle Vielfältigkeit von RNA wurde in d...

  14. Magnetic permeability based diagnostic test for the determination of the canine C-reactive protein concentration in undiluted whole blood

    Energy Technology Data Exchange (ETDEWEB)

    Ibraimi, Filiz; Kriz, Kirstin [Department of Pure and Applied Biochemistry, Lund University, P.O. Box 124, SE-221 00 Lund (Sweden); LifeAssays AB, IDEON Science Park, SE-223 70 Lund (Sweden); Merin, Henrik [Malmoe Animal Hospital, P.O. Box 9090, SE-213 63 Malmoe (Sweden); Kriz, Dario [Department of Pure and Applied Biochemistry, Lund University, P.O. Box 124, SE-221 00 Lund (Sweden); LifeAssays AB, IDEON Science Park, SE-223 70 Lund (Sweden)], E-mail: dario.kriz@euris.org

    2009-05-15

    We describe an one-step 11-min magnetic permeability based two-site immunoassay for C-reactive protein (CRP) utilizing polyclonal anti-canine CRP antibody conjugated dextran iron oxide nanoparticles (79 nm) as superparamagnetic labels and polyclonal anti-canine CRP conjugated silica microparticles (15 to 40 {mu}m) as carriers. An inductance based magnetic permeability reader was used to detect the target analyte, CRP, in 10 {mu}L whole blood samples, by measuring the magnetic permeability increase of the silica microparticle sediment due to immuno complex superparamagnetic nanoparticles. Measurements on standards showed a linear response between 0 and 17.5 mg/L CRP. Measurements performed on 16 whole blood samples from mixed breeds showed good correlation with a commercially available ELISA assay.

  15. Magnetic permeability based diagnostic test for the determination of the canine C-reactive protein concentration in undiluted whole blood

    International Nuclear Information System (INIS)

    We describe an one-step 11-min magnetic permeability based two-site immunoassay for C-reactive protein (CRP) utilizing polyclonal anti-canine CRP antibody conjugated dextran iron oxide nanoparticles (79 nm) as superparamagnetic labels and polyclonal anti-canine CRP conjugated silica microparticles (15 to 40 μm) as carriers. An inductance based magnetic permeability reader was used to detect the target analyte, CRP, in 10 μL whole blood samples, by measuring the magnetic permeability increase of the silica microparticle sediment due to immuno complex superparamagnetic nanoparticles. Measurements on standards showed a linear response between 0 and 17.5 mg/L CRP. Measurements performed on 16 whole blood samples from mixed breeds showed good correlation with a commercially available ELISA assay.

  16. Pattern recognition of monocyte chemoattractant protein-1 (MCP-1) in whole blood samples using new platforms based on nanostructured materials

    Science.gov (United States)

    Stefan-van Staden, Raluca-Ioana; Gugoasa, Livia Alexandra; Biris, Alexandru Radu

    2015-09-01

    Four stochastic microsensors based on nanostructured materials (graphene, maltodextrin (MD), and diamond) integrated in miniaturized platforms were proposed. Monocyte chemoattractant protein-1 (MCP-1) is a pro-inflammatory cytokine whose main function is to regulate cell trafficking. It is correlated with the incidence of cardiovascular diseases and obesity, and was used as the model analyte in this study. The screening of whole blood samples for MCP-1 can be done for concentrations ranging from 10-12 to 10-8 g mL-1. The method was used for both qualitative and quantitative assessments of MCP-1 in whole blood samples. The lowest quantification limits for the assay of MCP-1 (1 pg mL-1) were reached when the microsensors based on protoporphyrin IX/Graphene-Au-3 and on MD/Graphene were employed in the platform design.

  17. Blood and Milk Protein Polymorphism and Their Effects on Various Production Traits in Simmental Cows

    OpenAIRE

    ŞEKERDEN, Özel

    1998-01-01

    This research was carried out to determine the transferrin (Tf) and haemoglobine (Hb) and milk protein (alfa-S1-casein, beta casein, capa casein, beta lactoglobuline) types, and relationships between Tf and Hb types and milk protein genotypes with various characteristics. The relationship between Tf types and milk yields of control milking and control day were only statistically significant (P0.05). None of the investigated characteristics were effected by milk protein genetic variants at sta...

  18. Towards an animal model of ovarian cancer: cataloging chicken blood proteins using combinatorial peptide ligand libraries coupled with shotgun proteomic analysis for translational research.

    Science.gov (United States)

    Ma, Yingying; Sun, Zeyu; de Matos, Ricardo; Zhang, Jing; Odunsi, Kunle; Lin, Biaoyang

    2014-05-01

    Epithelial ovarian cancer is the most deadly gynecological cancer around the world, with high morbidity in industrialized countries. Early diagnosis is key in reducing its morbidity rate. Yet, robust biomarkers, diagnostics, and animal models are still limited for ovarian cancer. This calls for broader omics and systems science oriented diagnostics strategies. In this vein, the domestic chicken has been used as an ovarian cancer animal model, owing to its high rate of developing spontaneous epithelial ovarian tumors. Chicken blood has thus been considered a surrogate reservoir from which cancer biomarkers can be identified. However, the presence of highly abundant proteins in chicken blood has compromised the applicability of proteomics tools to study chicken blood owing to a lack of immunodepletion methods. Here, we demonstrate that a combinatorial peptide ligand library (CPLL) can efficiently remove highly abundant proteins from chicken blood samples, consequently doubling the number of identified proteins. Using an integrated CPLL-1DGE-LC-MSMS workflow, we identified a catalog of 264 unique proteins. Functional analyses further suggested that most proteins were coagulation and complement factors, blood transport and binding proteins, immune- and defense-related proteins, proteases, protease inhibitors, cellular enzymes, or cell structure and adhesion proteins. Semiquantitative spectral counting analysis identified 10 potential biomarkers from the present chicken ovarian cancer model. Additionally, many human homologs of chicken blood proteins we have identified have been independently suggested as diagnostic biomarkers for ovarian cancer, further triangulating our novel observations reported here. In conclusion, the CPLL-assisted proteomic workflow using the chicken ovarian cancer model provides a feasible platform for translational research to identify ovarian cancer biomarkers and understand ovarian cancer biology. To the best of our knowledge, we report here

  19. Cloning, expression and characterization of a gene from earthworm Eisenia fetida encoding a blood-clot dissolving protein.

    Science.gov (United States)

    Li, GangQiang; Wang, Kevin Yueju; Li, DaHui; Wang, Nan; Liu, DeHu

    2012-01-01

    A lumbrokinase gene encoding a blood-clot dissolving protein was cloned from earthworm (Eisenia fetida) by RT-PCR amplification. The gene designated as CST1 (GenBank No. AY840996) was sequence analyzed. The cDNA consists of 888 bp with an open reading frame of 729 bp, which encodes 242 amino acid residues. Multiple sequence alignments revealed that CST1 shares similarities and conserved amino acids with other reported lumbrokinases. The amino acid sequence of CST1 exhibits structural features similar to those found in other serine proteases, including human tissue-type (tPA), urokinase (uPA), and vampire bat (DSPAα1) plasminogen activators. CST1 has a conserved catalytic triad, found in the active sites of protease enzymes, which are important residues involved in polypeptide catalysis. CST1 was expressed as inclusion bodies in Escherichia coli BL21(DE3). The molecular mass of recombinant CST1 (rCST) was 25 kDa as estimated by SDS-PAGE, and further confirmed by Western Blot analysis. His-tagged rCST1 was purified and renatured using nickel-chelating resin with a recovery rate of 50% and a purity of 95%. The purified, renatured rCST1 showed fibrinolytic activity evaluated by both a fibrin plate and a blood clot lysis assay. rCST1 degraded fibrin on the fibrin plate. A significant percentage (65.7%) of blood clot lysis was observed when blood clot was treated with 80 mg/mL of rCST1 in vitro. The antithrombotic activity of rCST1 was 912 units/mg calculated by comparison with the activity of a lumbrokinase standard. These findings indicate that rCST1 has potential as a potent blood-clot treatment. Therefore, the expression and purification of a single lumbrokinase represents an important improvement in the use of lumbrokinases. PMID:23300872

  20. Proteins involved in the Vroman effect during exposure of human blood plasma to glass and polyethylene

    NARCIS (Netherlands)

    Turbill, P.; Beugeling, T.; Poot, A.A.

    1996-01-01

    The amounts of fibrinogen adsorbed to glass from various human blood plasmas have been measured as a function of time. The plasmas were 11 single donor plasmas, pooled plasma, a single donor high molecular weight kininogen (HMWK)-deficient plasma and HMWK-deficient plasma, which had been reconstitut

  1. Immunomodulatory capacity of fungal proteins on the cytokine production of human peripheral blood mononuclear cells

    NARCIS (Netherlands)

    Jeurink, P.V.; Lull Noguera, C.; Savelkoul, H.F.J.; Wichers, H.J.

    2008-01-01

    Immunomodulation by fungal compounds can be determined by the capacity of the compounds to influence the cytokine production by human peripheral blood mononuclear cells (hPBMC). These activities include mitogenicity, stimulation and activation of immune effector cells. Eight mushroom strains (Agaric

  2. High-protein and high-carbohydrate breakfasts differentially change the transcriptome of human blood cells

    NARCIS (Netherlands)

    Erk, M.J. van; Blom, W.A.M.; Ommen, B. van; Hendriks, H.F.J.

    2006-01-01

    Background: Application of transcriptomics technology in human nutrition intervention studies would allow for genome-wide screening of the effects of specific diets or nutrients and result in biomarker profiles. Objective: The aim was to evaluate the potential of gene expression profiling in blood c

  3. SDS-PAGE Patterns of Blood Serum Proteins in some Species of the Genus Meriones (Mammalia: Rodentia)

    OpenAIRE

    ÇOLAK, Reyhan; Nuri YİĞİT; ÇOLAK, Ercüment

    2002-01-01

    The blood serum proteins of Meriones meridianus, Meriones crassus, Meriones persicus and Meriones tristrami, all found in Turkey, along with domestic Meriones unguiculatus, which originated in Mongolia, were examined for the first time by SDS-PAGE. In the globulin zone, seven to ten bands were identified, one band in the post-albumin and albumin zones and one or two bands in the pre-albumin zone of five species of the genus Meriones. There was no diagnostic difference in the pattern of serum ...

  4. Die Rolle des neuentwickelten pYD-Vectors bei der Entwicklung eines Selektionssystems zum Screening von cDNA Bibliotheken auf sezernierte Proteine und Membranproteine

    OpenAIRE

    Duran, Mansur

    2010-01-01

    Introduction: The purpose of this work is to be represented the role of the pYD vector in the yeast invertase screening system for isolating secreted proteins and membrane proteins. The examination of the sensitivity and specificity of the system should be accomplished with a human microvascular endothelial cell library. At the same time new molecules, which play a function in the angiogenesis, should be found. Receptors, membrane proteins, and secreted proteins, are of considerable intere...

  5. An occludin-focal adhesion kinase protein complex at the blood-testis barrier: a study using the cadmium model.

    Science.gov (United States)

    Siu, Erica R; Wong, Elissa W P; Mruk, Dolores D; Sze, K L; Porto, Catarina S; Cheng, C Yan

    2009-07-01

    Several integral membrane proteins that constitute the blood-testis barrier (BTB) in mammalian testes, in particular rodents, are known to date. These include tight junction (TJ) proteins (e.g. occludin, junctional adhesion molecule-A, claudins), basal ectoplasmic specialization proteins (e.g. N-cadherin), and gap junction proteins (e.g. connexin43). However, the regulators (e.g. protein kinases and phosphatases) that affect these proteins, such as their interaction with the cytoskeletal actin, which in turn confer cell adhesion at the TJ, remain largely unknown. We report herein that focal adhesion kinase (FAK) is a putative interacting partner of occludin, but not claudin-11 or junctional adhesion molecule-A. Immunohistochemistry and fluorescence microscopy studies illustrated that the expression of FAK in the seminiferous epithelium of adult rat testes was stage specific. FAK colocalized with occludin at the BTB in virtually all stages of the seminiferous epithelial cycle but considerably diminished in stages VIII-IX, at the time of BTB restructuring to facilitate the transit of primary leptotene spermatocytes. Using Sertoli cells cultured in vitro with established TJ-permeability barrier and ultrastructures of TJ, basal ectoplasmic specialization and desmosome-like junction that mimicked the BTB in vivo, FAK was shown to colocalize with occludin and zonula occludens-1 (ZO-1) at the Sertoli-Sertoli cell interface. When these Sertoli cell cultures were treated with CdCl(2) to perturb the TJ-barrier function, occludin underwent endocytic-mediated internalization in parallel with FAK and ZO-1. Thus, these findings demonstrate that FAK is an integrated regulatory component of the occludin-ZO-1 protein complex, suggesting that functional studies can be performed to study the role of FAK in BTB dynamics. PMID:19213829

  6. Effect of Buddhist meditation on serum cortisol and total protein levels, blood pressure, pulse rate, lung volume and reaction time.

    Science.gov (United States)

    Sudsuang, R; Chentanez, V; Veluvan, K

    1991-09-01

    Serum cortisol and total protein levels, blood pressure, heart rate, lung volume, and reaction time were studied in 52 males 20-25 years of age practicing Dhammakaya Buddhist meditation, and in 30 males of the same age group not practicing meditation. It was found that after meditation, serum cortisol levels were significantly reduced, serum total protein level significantly increased, and systolic pressure, diastolic pressure and pulse rate significantly reduced. Vital capacity, tidal volume and maximal voluntary ventilation were significantly lower after meditation than before. There were also significant decreases in reaction time after mediation practice. The percentage decrease in reaction time during meditation was 22%, while in subjects untrained in meditation, the percentage decrease was only 7%. Results from these studies indicate that practising Dhammakaya Buddhist meditation produces biochemical and physiological changes and reduces the reaction time. PMID:1801007

  7. Differential proteolytic activation of factor VIII-von Willebrand factor complex by thrombin

    International Nuclear Information System (INIS)

    Blood coagulation factor VIII (fVIII) is a plasma protein that is decreased or absent in hemophilia A. It is isolated as a mixture of heterodimers that contain a variably sized heavy chain and a common light chain. Thrombin catalyzes the activation of fVIII in a reaction that is associated with cleavages in both types of chain. The authors isolated a serine protease from Bothrops jararacussu snake venom that catalyzes thrombin-like heavy-chain cleavage but not light-chain cleavage in porcine fVIII as judged by NaDodSO4/PAGE and N-terminal sequence analysis. Using a plasma-free assay of the ability of activated 125I-fVIII to function as a cofactor in the activation of factor X by factor IXa, they found that fVIII is activated by the venom enzyme. The venom enzyme-activated fVIII was isolated in stable form by cation-exchange HPLC. von Willebrand factor inhibited venom enzyme-activated fVIII but not thrombin-activated fVIII. These results suggest that the binding of fVIII to von Willebrand factor depends on the presence of an intact light chain and that activated fVIII must dissociate from von Willebrand factor to exert its cofactor effect. Thus, proteolytic activation of fVIII-von Willebrand factor complex appears to be differentially regulated by light-chain cleavage to dissociate the complex and heavy-chain cleavage to activate the cofactor function

  8. Differences in abundances of cell-signalling proteins in blood reveal novel biomarkers for early detection of clinical Alzheimer's disease.

    Directory of Open Access Journals (Sweden)

    Mateus Rocha de Paula

    Full Text Available BACKGROUND: In November 2007 a study published in Nature Medicine proposed a simple test based on the abundance of 18 proteins in blood to predict the onset of clinical symptoms of Alzheimer's Disease (AD two to six years before these symptoms manifest. Later, another study, published in PLoS ONE, showed that only five proteins (IL-1, IL-3, EGF, TNF- and G-CSF have overall better prediction accuracy. These classifiers are based on the abundance of 120 proteins. Such values were standardised by a Z-score transformation, which means that their values are relative to the average of all others. METHODOLOGY: The original datasets from the Nature Medicine paper are further studied using methods from combinatorial optimisation and Information Theory. We expand the original dataset by also including all pair-wise differences of z-score values of the original dataset ("metafeatures". Using an exact algorithm to solve the resulting Feature Set problem, used to tackle the feature selection problem, we found signatures that contain either only features, metafeatures or both, and evaluated their predictive performance on the independent test set. CONCLUSIONS: It was possible to show that a specific pattern of cell signalling imbalance in blood plasma has valuable information to distinguish between NDC and AD samples. The obtained signatures were able to predict AD in patients that already had a Mild Cognitive Impairment (MCI with up to 84% of sensitivity, while maintaining also a strong prediction accuracy of 90% on a independent dataset with Non Demented Controls (NDC and AD samples. The novel biomarkers uncovered with this method now confirms ANG-2, IL-11, PDGF-BB, CCL15/MIP-1; and supports the joint measurement of other signalling proteins not previously discussed: GM-CSF, NT-3, IGFBP-2 and VEGF-B.

  9. Mein Traum von Bibliothek

    OpenAIRE

    Bauer, Charlotte; Schneider, Ulrich Johannes

    2011-01-01

    Unter dem Titel „Mein Traum von Bibliothek“ wird demnächst an der Universitätsbibliothek Leipzig eine Vortragsreihe für Bibliothekare starten. Thema ist der von uns allen erlebte rasante Wandel in der Mediennutzung durch digitale Technik. Die Aufgaben der Bibliothek ändern sich, die Tätigkeiten der Bibliothekare ändern sich, die Funktionen der Bibliotheksräume ändern sich. Das hat Konsequenzen für alle wissenschaftlichen Bibliotheken, ins besondere für ein komplexes System wie das der UB Leip...

  10. Transformation von Zierpflanzen

    OpenAIRE

    Börstling, Dirk

    2005-01-01

    Da die Gentechnik und die Molekularbiologie für die moderne Pflanzenzüchtung in den letzten Jahren enorm an Bedeutung gewonnen haben, besteht auch für die Züchtung von gartenbaulichen Nutzpflanzen ein Interesse am Einsatz dieser Methoden. Die erfolgreiche Veränderung der Blütenfarbe bei Petunia hybrida (Meyer et al. 1987) stellt den Anfang der Transformation von Zierpflanzen mit einem konkreten und wirtschaftlich relevanten Ziel dar. Die Ergebnisse dienen nicht nur der Grundlagenwissenschaft,...

  11. Kontextualisierung von Queer Theory

    Directory of Open Access Journals (Sweden)

    Anna Voigt

    2008-03-01

    Full Text Available Christine M. Klapeer legt in diesem Einführungsband dar, aus welchen politischen und theoretischen Kontexten heraus sich ‚queer‘ zu einem Begriff mit besonderem politischem und theoretischem Gehalt entwickelt hat. Wesentlich zielt sie dabei auf eine kritische Kontextualisierung von „queer theory”. Die Autorin geht zunächst auf das Gay Liberation Movement ein, grenzt die Queer Theory vom Poststrukturalismus, von feministischen Theorien und den Lesbian and Gay Studies ab, beleuchtet Eckpunkte queeren Denkens und zeichnet schließlich die Entwicklungen in Österreich sowohl politisch-rechtlich als auch bewegungsgeschichtlich und in der Wissenschaftslandschaft nach.

  12. A micro-fluidic study of whole blood behaviour on PMMA topographical nanostructures

    Directory of Open Access Journals (Sweden)

    Tsud Nataliya

    2008-02-01

    Full Text Available Abstract Background Polymers are attractive materials for both biomedical engineering and cardiovascular applications. Although nano-topography has been found to influence cell behaviour, no established method exists to understand and evaluate the effects of nano-topography on polymer-blood interaction. Results We optimized a micro-fluidic set-up to study the interaction of whole blood with nano-structured polymer surfaces under flow conditions. Micro-fluidic chips were coated with polymethylmethacrylate films and structured by polymer demixing. Surface feature size varied from 40 nm to 400 nm and feature height from 5 nm to 50 nm. Whole blood flow rate through the micro-fluidic channels, platelet adhesion and von Willebrand factor and fibrinogen adsorption onto the structured polymer films were investigated. Whole blood flow rate through the micro-fluidic channels was found to decrease with increasing average surface feature size. Adhesion and spreading of platelets from whole blood and von Willebrand factor adsorption from platelet poor plasma were enhanced on the structured surfaces with larger feature, while fibrinogen adsorption followed the opposite trend. Conclusion We investigated whole blood behaviour and plasma protein adsorption on nano-structured polymer materials under flow conditions using a micro-fluidic set-up. We speculate that surface nano-topography of polymer films influences primarily plasma protein adsorption, which results in the control of platelet adhesion and thrombus formation.

  13. Tether Extrusion from Red Blood Cells: Integral Proteins Unbinding from Cytoskeleton

    OpenAIRE

    Borghi, N.; Brochard-Wyart, F.

    2007-01-01

    We investigate the mechanical strength of adhesion and the dynamics of detachment of the membrane from the cytoskeleton of red blood cells (RBCs). Using hydrodynamical flows, we extract membrane tethers from RBCs locally attached to the tip of a microneedle. We monitor their extrusion and retraction dynamics versus flow velocity (i.e., extrusion force) over successive extrusion-retraction cycles. Membrane tether extrusion is carried out on healthy RBCs and ATP-depleted or -inhibited RBCs. For...

  14. Effect of a high-protein diet on maintenance of blood pressure levels achieved after initial weight loss: the DiOGenes randomized study

    NARCIS (Netherlands)

    Engberink, M.F.; Geleijnse, J.M.; Bakker, S.J.L.; Larsen, T.

    2015-01-01

    Randomized trials have shown significant blood pressure (BP) reductions after increased protein compared with carbohydrate intake, but the effect on BP maintenance after initial weight loss is unclear. We examined the effect of a high-protein diet on the maintenance of reduced BP after weight loss i

  15. Effect of a high-protein diet on maintenance of blood pressure levels achieved after initial weight loss : the DiOGenes randomized study

    NARCIS (Netherlands)

    Engberink, M. F.; Geleijnse, J. M.; Bakker, S. J. L.; Larsen, T. M.; Handjieva-Darlesnka, T.; Kafatos, A.; Martinez, J. A.; Pfeiffer, A. F. H.; Kunesova, M.; Jebb, S. A.; Holst, C.; Astrup, A.; Saris, W. H. M.; Brink, E. J.; van Baak, M. A.

    2015-01-01

    Randomized trials have shown significant blood pressure (BP) reductions after increased protein compared with carbohydrate intake, but the effect on BP maintenance after initial weight loss is unclear. We examined the effect of a high-protein diet on the maintenance of reduced BP after weight loss i

  16. SU-E-T-45: Antibody Mean Residence Time in Blood and Its Correlation with Protein Molecular Weight

    International Nuclear Information System (INIS)

    Purpose: Animal biodistribution data are required prior to introducing a new radiopharmaceutical into clinical trials. Protein engineering, using recombinant DNA techniques can produce a large number of related (cognate) antibodies to a given molecular target. Thus, it is important that these constructs be numerically related to one another via a single criterion. In the following, we use the mean residence time (MRT) in murine blood as this criterion. Methods: Five cognate anti-CEA (Carcinoembryonic Antigen) antibodies were compared with regard to their MRT in whole blood of CEA-positive tumor-bearing (LS174T) mice. MRT was defined by blood AUC (area under the curve) divided by the initial blood uptake value; all in units of percent injected dose per gram (%ID/g). Cognates included single chain scFv (25 kDa), diabody (50 kDa), minibody (80 kDa), F(ab')2 (120 kDa), and intact (155 kDa) forms of the murine cT84.66 antibody against CEA. All were labeled with radioactive iodine. Results: The agents, in the sequence listed, exhibited MRT values of 1.16 +/- 0.01 h, 0.99 h, 5.06 +/- 0.70 h, 6.61 +/- 0.36 h, and 59.3 +/- 2.4 h respectively. Because of the monotonic nature of the sequence, a linear correlation analysis was performed between molecular weight (MW) and MRT or ln(MRT) of the 5 proteins. Probability of random correlation was 0.10 for MRT and 0.01 for ln(MRT). Conclusion: MRT values of cognate anti-CEA antibodies were found to be a monotonically increasing sequence with respect to MW. Cognate MW values correlated best to ln(MRT) of the protein species. Thus MRT was proportional to an exponential function of molecular weight. The extended intact antibody circulation time presumably reflected its relatively maximal MW. Presence of an intact FC segment on this native antibody may also have influenced these results

  17. SU-E-T-45: Antibody Mean Residence Time in Blood and Its Correlation with Protein Molecular Weight

    Energy Technology Data Exchange (ETDEWEB)

    Kwok, C; Williams, L [Retired from City of Hope Medical Center, Arcadia, CA (United States)

    2014-06-01

    Purpose: Animal biodistribution data are required prior to introducing a new radiopharmaceutical into clinical trials. Protein engineering, using recombinant DNA techniques can produce a large number of related (cognate) antibodies to a given molecular target. Thus, it is important that these constructs be numerically related to one another via a single criterion. In the following, we use the mean residence time (MRT) in murine blood as this criterion. Methods: Five cognate anti-CEA (Carcinoembryonic Antigen) antibodies were compared with regard to their MRT in whole blood of CEA-positive tumor-bearing (LS174T) mice. MRT was defined by blood AUC (area under the curve) divided by the initial blood uptake value; all in units of percent injected dose per gram (%ID/g). Cognates included single chain scFv (25 kDa), diabody (50 kDa), minibody (80 kDa), F(ab')2 (120 kDa), and intact (155 kDa) forms of the murine cT84.66 antibody against CEA. All were labeled with radioactive iodine. Results: The agents, in the sequence listed, exhibited MRT values of 1.16 +/- 0.01 h, 0.99 h, 5.06 +/- 0.70 h, 6.61 +/- 0.36 h, and 59.3 +/- 2.4 h respectively. Because of the monotonic nature of the sequence, a linear correlation analysis was performed between molecular weight (MW) and MRT or ln(MRT) of the 5 proteins. Probability of random correlation was 0.10 for MRT and 0.01 for ln(MRT). Conclusion: MRT values of cognate anti-CEA antibodies were found to be a monotonically increasing sequence with respect to MW. Cognate MW values correlated best to ln(MRT) of the protein species. Thus MRT was proportional to an exponential function of molecular weight. The extended intact antibody circulation time presumably reflected its relatively maximal MW. Presence of an intact FC segment on this native antibody may also have influenced these results.

  18. The effects of stress-induced blood components on protein synthesis and secretion in isolated rat hepatocytes

    International Nuclear Information System (INIS)

    The effect of stress-induced blood components were examined, specifically adrenaline and noradrenaline, in the presence and absence of rabbit serum or foetal calf serum, on soluble protein synthesis and secretion by isolated hepatocytes maintained in monolayer culture. Rabbit serum and low doses of adrenaline stimulated soluble protein synthesis and secretion whereas foetal calf serum and high doses of noradrenaline were inhibitory. The effect of noradrenaline on soluble protein synthesis and secretion ocurred in the first 12 hours of incubation. The stimulatory effect of adrenaline was still present after 24 hours of incubation. Preloading of the medium with [3H]-leucine i.e. before the addition of sera and/or catecholamines, showed the [3H]-leucine uptake to have occured to a large extent within the first hour of incubation. Noradrenaline supplementation of the medium at two hourly intervals showed no effect on protein synthesis and secretion. The stability of the cetecholamines and the status of the receptors need to be determined for the effective analysis of the results at any point during the incubation. 17 figs., 15 tabs., 83 refs

  19. Proteomic analysis of blood level of proteins before and after operation in patients with esophageal squamous cell carcinoma at high-incidence area in Henan Province

    Institute of Scientific and Technical Information of China (English)

    Ji-Ye An; Zong-Min Fan; Ze-Hao Zhuang; Yan-Ru Qin; Shan-Shan Gao; Ji-Lin Li; Li-Dong Wang

    2004-01-01

    AIM: To characterize the protein files in blood from same patients with esophageal squamous cell carcinoma (ESCC)before and after operation at the high-incidence area for ESCC in Henan Province, China.METHODS: Two-dimensional electrophoresis, silver staining and ImageMaster 2-DE analysis software were applied to the determination of protein files in the blood obtained from normal controls and ESCC patients before and after operation.RESULTS: A total of 655, 662 and 677 protein spots were identified, respectively, from the normal controls and ESCC patients before and after operation. No significant difference in the number of protein spots was observed between the normal group and ESCC patients. A total of seven protein spots were identified with a dramatic difference among the samples before and after operation. Six protein spots were up-regulated and one protein spot was down-regulated in the group after operation compared with those in normal and before operation. Three protein spots were further characterized by matrix-assisted laser desorption/ionization time of flying mass spectrometry (MALDI-TOF-MS). The proteins from these three spots were identified as serum amyloid A(SAA), amyloid related serum protein and haptoglobin.CONCLUSION: Serum amyloid A, amyloid related serum protein and haptoglobin may be related with ESCC and/or surgery. The significance of these proteins needs to be further characterized. The present study provides informative data for the establishment of serum protein profiles related with ESCC.

  20. Massenspektrometrische Identifizierung von Membranproteinen aus humanen Spermatozoen

    OpenAIRE

    Bartho, Kathrin

    2010-01-01

    Humane Spermatozoen sind extrazellulär glykosylierte Zellen, die von einer Plasmamembran umgeben werden. Diese Lipiddoppelschicht bildet eine natürliche Barriere zwischen Zellinnenraum und dem extrazellulären Milieu. In diese Membran sind essentielle Proteine für Chemotaxis, Signaltransduktion und den Fertilisationsprozess integriert. Im Fokus der Arbeit steht die massenspektrometrische Identifizierung niedrig abundanter olfaktorischer Rezeptoren. Erstmalig konnten 59 olfaktorisch...

  1. Effect of advanced glycosylation end products on activity of protein kinase C in human peripheral blood mononuclear cells

    Institute of Scientific and Technical Information of China (English)

    2000-01-01

    Objectives TO investigate the effect of advanced glycosylation end products (AGEs) on the activity of protein kinese C (PKC) in human peripheral bloodmononuclear Cells (PBMC) and to observe whether aminoguanidine (AG) can influence the effect of AGEs. Methods After PBMC were isoiated from human peripheral blood and incubated with different concentrations of AGEs-BSA for various periods, total PKC activity in PBMC was determined by measuring the incorporation of 32P from [γ-32P] ATP=into a special substrate using Prornega PKC assay kit. Results AGEs-BSA increased the total PKC activity in PBMC from 83.43±6.57 pmol/min/mg protein to 116.8±13.82 pmol/min/mg protein with a peak at 15 min.AGEs-BSA also increased the total PKC activity in a concentration-dependent manner from 83.1±6.4 pmol/min/mg protein(control) to 119.1±13.3 pmol/min/mg protein (control vs AGEs-BSA 400 mg/L, P<0.01). Furthermore, AGEs-BSA induced an elevation of PKC activity in a glycosylating time-related manner,from 80.9±8.2 (control) to 118.3±11.5 pmol/min/mg protein (glycasytation for 12 wk, P<0.01). The total PKC activity stimulated by AGEs-BSA pretreated with AG (100, 200 mg/L) was markedly lower than that of AGEs-BSA group not pretreated with AG ( P<0.05, P<0.01). Conclusions AGEs-BSA increased the total PKC activity in PBMC in a concentration and incubation time dependent manner. The ability of AGEs-B.SA to stimulate PKC activity was markedly decreased by pretreatment of AGEs-BSA with AG.

  2. Influence of sinomenine on protein profiles of peripheral blood mononuclear cells from ankylosing spondylitis patients: a pharmacoproteomics study

    Institute of Scientific and Technical Information of China (English)

    HUANG Zhi-xiang; TAN Jin-hui; LI Tian-wang; DENG Wei-ming; QIU Ke-wei; LIAO Ze-tao; ZENG Zhao-qiu

    2013-01-01

    Background Ankylosing spondylitis (AS) is a common inflammatory rheumatic disease which lacks satisfactory treatment so far.Sinomenine (SIN) is an alkaloid and has recently been utilized in treating multiple rheumatic diseases including AS in China,but its exact mechanism remains to be explored.This study investigated the alteration of proteome in peripheral blood mononuclear cells (PBMCs) from AS patients.Methods Thirty AS patients were enrolled in this study.PBMCs from each AS patient were cultured in medium with or without SIN respectively.Then PBMCs proteins from both groups were separated by two-dimensional electrophoresis (2-DE) and analyzed by mass spectrometry (MS).Two differentially expressed proteins were then chosen to be verified using Western blotting.Results Seven proteins,including α-synuclein (SNCA),calmodulin (CALM),acidic leucine-rich nuclear phosphoprotein 32 family member A (ANP32A),chloride intracellular channel protein 1 (CLIC1),guanine nucleotide-binding protein G(I)/G(S)/ G(T) subunit beta-1 (GNB1),gelsolin (GSN) and histone H2B type 1-M (HISTH2BM)were over-expressed,while coronin1A (CORO1A) was under-expressed in the SIN-treated PBMCs.Further bioinformatics search indicated that the changes of SNCA,ANP32A and CLIC1 pertained to apoptosis,while changes of GSN and CORO1A were associated with both apoptosis and inhibition of immunological function.Subsequently GSN and CORO1A were selected to validate by Western blotting and the results were consistent with those of 2-DE.Conclusion There were 8 differentially expressed proteins in the SIN-treated PBMCs,which might shed some light on the mechanism of SIN in the treatment of AS.

  3. Pseudo (Platelet-type) von Willebrand disease in pregnancy: a case report

    OpenAIRE

    Grover Neetu; Boama Vincent; Chou Munazzah Rifat

    2013-01-01

    Abstract Background Pseudo (platelet-type)-von Willebrand disease is a rare autosomal dominant bleeding disorder caused by an abnormal function of the glycoprotein lb protein; the receptor for von Willebrand factor. This leads to an increased removal of VWF multimers from the circulation as well as platelets and this results in a bleeding diathesis. Worldwide, less than 50 patients are reported with platelet type von Willebrand disease (PT-VWD). Case presentation We describe the management of...

  4. Funktionelle Untersuchungen zum Einfluss von FAP-Inhibitoren auf die Tumorzellmigration und Invasion

    OpenAIRE

    Kirchner, Paul

    2005-01-01

    Das Fibroblasten-Aktivierungs-Protein (FAP) ist eine Oberflaechenprotease mit kollagenolytischer Aktivität in vitro, die in vivo spezifisch von Fibroblasten in der Umgebung maligner epithelialer Tumoren in der Phase der Invasion und Disseminierung aufreguliert wird. Da Matrixproteasen durch proteolytischen Umbau extrazellulärer Matrix zu Invasion und Metastasierung von Tumoren beitragen, stellen sie interessante Zielstrukturen für die Pharmakotherapie von Tumorerkrankungen dar....

  5. Spezielle Anwendungen von EMDR

    OpenAIRE

    Schubbe, Oliver; Püschel, Ines; Renssen, Monique

    2013-01-01

    Nach traumatischen Erlebnissen können sich neben der Symptomatik einer Posttraumatischen Belastungsstörung verschiedene klinische Störungsbilder entwickeln. Diese lassen sich in Abhängigkeit von der individuellen Bewältigungsform schematisch vereinfacht beschreiben.

  6. Qualitative Beschreibung von Automatisierungssoftware

    OpenAIRE

    Biegert, Uwe

    1998-01-01

    Für die Entwicklung sicherer Automatisierungssysteme werden eine Reihe von konventionellen Methoden eingesetzt, die jeweils einzelne Bestandteile eines Systems separat betrachten. So wird der technische Prozeß mit Verfahren wie FMEA, PAAG oder der Fehlerbaumanalyse auf sicherheitskritische Aspekte untersucht. Bei der Automatisierungssoftware werden Validierungs- oder Verifikationsmethoden verwendet. Das Verhalten des Menschen wird auf einzelne Aktionen heruntergebrochen und ...

  7. Insolvenzschutz von Wertguthaben

    OpenAIRE

    Hanau, Peter; Rolfs, Christian

    2003-01-01

    Arbeitszeitkonten stellen einen wichtigen Bestandteil der Flexibilisierung von Arbeitszeiten in Deutschland dar. Sie dienen den Interessen der Arbeitnehmerinnen und Arbeitnehmer wie der Arbeitgeber in gleicher Weise. Die Tarifvertrags- und Betriebsparteien haben vielfältige, den spezifischen Belangen der jeweiligen Branchen und Unternehmen Rechnung tragende Modelle entwickelt. Die legislativen Rahmenbedingungen zur Fortentwicklung flexibler Arbeitszeitmodelle wurden durch den Gesetzgeber scho...

  8. Fractal von Neumann entropy

    OpenAIRE

    da Cruz, Wellington

    2002-01-01

    We consider the {\\it fractal von Neumann entropy} associated with the {\\it fractal distribution function} and we obtain for some {\\it universal classes h of fractons} their entropies. We obtain also for each of these classes a {\\it fractal-deformed Heisenberg algebra}. This one takes into account the braid group structure of these objects which live in two-dimensional multiply connected space.

  9. Anpassung von individuellen Mediennutzungswahrscheinlichkeiten

    OpenAIRE

    Kopp, Bruno

    2011-01-01

    Es wird eine Anpassungsmethode von Nutzungswahrscheinlichkeiten bei Umfragen zur Mediennutzung untersucht. Das Verfahren ist so angelegt, dass die Reichweiten in vorgegebenen Zielgruppen an Sollwerten justiert werden. Ein Kontrollparameter sorgt dafür, dass die ursprünglichen Nutzungswahrscheinlichkeiten möglichst wenig verändert werden.

  10. Ellipsometric studies of synthetic albumin-binding chitosan-derivatives and selected blood plasma proteins

    Science.gov (United States)

    Sarkar, Sabyasachi

    This dissertation summarizes work on the synthesis of chitosan-derivatives and the development of ellipsometric methods to characterize materials of biological origin. Albumin-binding chitosan-derivatives were synthesized via addition reactions that involve amine groups naturally present in chitosan. These surfaces were shown to have an affinity towards human serum albumin via ELISA, UV spectroscopy and SDS PAGE. Modified surfaces were characterized with IR ellipsometry at various stages of their synthesis using appropriate optical models. It was found that spin cast chitosan films were anisotropic in nature. All optical models used for characterizing chitosan-derivatives were thus anisotropic. Chemical signal dependence on molecular structure and composition was illustrated via IR spectroscopic ellipsometry (IRSE). An anisotropic optical model of an ensemble of Lorentz oscillators were used to approximate material behavior. The presence of acetic acid in spin-cast non-neutralized chitosan samples was thus shown. IRSE application to biomaterials was also demonstrated by performing a step-wise chemical characterizations during synthesis stages. Protein adsorbed from single protein solutions on these modified surfaces was monitored by visible in-situ variable wavelength ellipsometry. Based on adsorption profiles obtained from single protein adsorption onto silicon surfaces, lumped parameter kinetic models were developed. These models were used to fit experimental data of immunoglobulin-G of different concentrations and approximate conformational changes in fibrinogen adsorption. Biomaterial characterization by ellipsometry was further extended to include characterization of individual protein solutions in the IR range. Proteins in an aqueous environment were characterized by attenuated total internal reflection (ATR) IR ellipsometry using a ZnSe prism. Parameterized dielectric functions were created for individual proteins using Lorentz oscillators. These

  11. Effect of dietary protein content on animal production and blood metabolites of dairy cows during lactation.

    Science.gov (United States)

    Law, R A; Young, F J; Patterson, D C; Kilpatrick, D J; Wylie, A R G; Mayne, C S

    2009-03-01

    Ninety autumn-calving Holstein dairy cows [45 primiparous and 45 multiparous (mean parity, 3.1)] were allocated to 1 of 3 dietary crude protein (CP) concentrations: 173, 144, or 114 g of CP/kg of DM, from calving until d 150 of lactation. On d 151, half of the animals in each treatment were allocated an alternative dietary protein concentration. Half of the animals receiving 114 g of CP/kg of DM went onto 144 g of CP/kg of DM; half of the animals receiving 144 g of CP/kg of DM went onto 173 g of CP/kg of DM; and half of the animals receiving 173 g of CP/kg of DM went onto 144 g of CP/kg of DM, with the remaining animals staying on their original treatment. This resulted in 6 treatments in the mid to late lactation period: 114/114, 144/144, 173/173, 114/144, 144/173, and 173/144 g of CP/kg of DM. An increase in dietary CP concentration significantly increased milk, fat, and protein yield in early lactation (d 1 to 150). Dry matter intake was also increased with increased dietary protein concentration; however, this was not significant between 144 and 173 g of CP/kg of DM. Increased dietary CP significantly increased plasma urea, albumin, and total protein concentrations but had no significant effect on NEFA, leptin, or IGF-1 concentrations. Decreasing the dietary CP concentration in mid-late lactation (d 151 to 305) from 173 to 144 g/kg of DM had no significant effect on milk yield, dry matter intake, or milk fat and protein yield, compared with animals that remained on 173 g of CP/kg of DM throughout lactation. Increasing dietary CP concentration from 144 to 173 g/kg of DM significantly increased dry matter intake compared with animals that remained on the 144 g of CP/kg of DM throughout lactation. There were no significant dietary treatment effects on live weight or body condition score change throughout the experiment. Results of this study indicate that high protein diets (up to 173 g of CP/kg of DM) improved feed intake and animal performance in early lactation

  12. The Effect of 60Co Gamma Irradiation on Various Fractions of Human Blood-Plasma Proteins

    International Nuclear Information System (INIS)

    The potential usefulness of employing ionizing radiation to cold-sterilize biomedical products has stimulated interest in characterizing the radiation sensitivity of various biologicals like vitamins, antibiotics, enzymes, antibodies and blood plasma fractions. This report presents findings on the sensitivity of haemagglutinin activity in human sera exposed to cobalt-60 gamma radiation. At doses tested between 0.2 and 5.0 Mrad, α-isohaemagglutinins were found to be more readily inactivated than ß-isohaemagglutinins, but neither was completely inactivated in this range. Sterility, clotting and antihaemophilic activity were also assessed on irradiated and freeze-dried preparations of human plasma with no significant differences in response detected. (author)

  13. Ontogeny and characterization of blood leukocyte subsets and serum proteins in piglets before and after weaning

    DEFF Research Database (Denmark)

    Juul-Madsen, H.R.; Jensen, K.H.; Nielsen, Jens;

    2010-01-01

    Existing knowledge about the development of the porcine immune system was extended by phenotypic characterization of leukocyte subsets and with assessment of Mannan-Binding Lectin (MBL) and immunoglobulin concentrations in peripheral blood of healthy piglets. Single-color and/or double-color flow...... immune system seem to be stimulated immediately after weaning. At the time considered to have the highest infection pressure T-cells and TLR4+ cells were markedly enhanced, whereas the expression of SLA I did not seem to be affected by weaning....

  14. C-14-activity incorporation into the protein of fetal organs of guinea pigs with different maternal placental blood flow and fetal arterial O2-saturation

    International Nuclear Information System (INIS)

    In anaesthesised gravid guinea-pigs the dilate, end section of a placental radial artery was connected to the A.carotis via a flow meter and a throttle in order to measure and widely alter the maternal placental blood flow. Blood samples are taken from the fetal A.carotis, the fetal arterial O2-saturation and the Hb-content were determined. By altering the maternal placental blood circulation the fetal arterial O2-concentration can stabilised at various levels. In order to study the protein synthesis, under these conditions one infused 185 kBq C-14-leucine over a period of 3 hours into the jugular vein of the fetus. During infusion the radioactive concentrations in whole plasma and plasma water were measured. After the infusion the radioactive concentrations in the tissue fluid, the intracellular fluid and the acid-insoluble tissue fraction (protein) of the heart, kidenys, liver, the muscles of the upper end lower part of the body, the brain and the placenta were measured. The following deductions were drawn from the findings: The maternal placental blood flow vitally influences the activity incorporation per activity concentration in the plasma water. These findings agree with the hypotheses that the maternal blood circulation has an essential influence on the fetal proteins synthesis and that this influence can be attributed to the connection between placenta connection blood flow and oxygen saturation of fetal arterial blood. (orig.)

  15. The structure of multilayer protein/polyelectrolyte coatings and its effect on blood compatibility

    Czech Academy of Sciences Publication Activity Database

    Houska, Milan; Brynda, Eduard; Broučková, A.; Dyr, J. E.

    Praha : Institute of Macromolecular Chemistry, AS CR, 2004 - (Kahovec, J.). s. PC38 ISBN 80-85009-49-8. [Microsymposium on Polymer Biomaterials: Biomimetic and Bioanalogous systems /43./. 11.07.2004-15.07.2004, Prague] R&D Projects: GA AV ČR KSK4050111 Keywords : protein/ polyelectrolyte assemblies * heparin * albumin Subject RIV: CD - Macromolecular Chemistry

  16. Blood protein polymorphism in three sheep breeds from the south of Tunisia

    Directory of Open Access Journals (Sweden)

    Khaldi Zahrane,

    2011-02-01

    Full Text Available Biochemical polymorphism was studied in the native ovine Barbarine (BAR and Queue Fine de l’Ouest (QF breeds as well as in the exotic D’man (DMN breed in the South of Tunisia using a panel of six protein coding loci: Haemoglobin (Hb, Carbonic anhydrase (Ca, Albumin (Al, Transferrin (Tf, X-protein (X-p and Arylesterase A (EsA. Polymorphic loci were detected by means of horizontal starch gel electrophoresis. All tested loci were polymorphic. The Tf locus exhibited the highest number of alleles while the other loci showed two alleles in all sampled breeds. Estimates of expected heterozygosity were almost twice those of observed heterosigositiy in all sheep populations and the high level of heterozygosity was observed in DMN breed. Genetic distances observed between BAR-DMN and between QF-DMN were larger than those found between BAR-QF breeds.

  17. Genetic and protein biomarkers in blood for the improved detection of GH abuse.

    Science.gov (United States)

    Ferro, P; Ventura, R; Pérez-Mañá, C; Farré, M; Segura, J

    2016-09-01

    Human Growth Hormone (hGH, somatotropin) is one of the relevant forbidden substances to be detected in sport drug testing. Since the appearance of recombinant hGH (rhGH) in the 80's, its expansion and availability through the black market have increased, so the detection of its abuse continues to be a challenge at present. New techniques or biomarkers that are robust, reliable, sensitive and allowing a large detection time window are welcome. rhGH produces an increase of insulin-like growth factor 1 (IGF-1). FN1 (fibronectin 1) and RAB31 (member of RAS oncogene family) genes have been suggested as two potential biomarkers for IGF-1 abuse. Following this line, in the present study some genetic and proteomic approaches have been performed with fourteen healthy male subjects treated with rhGH (which produces increase of IGF-1 concentrations) to study FN1 gene, FN1 protein, RAB31 gene and RAB31 protein as potential biomarkers for rhGH abuse. The results showed that both, RAB31 and FN1 genes and FN1 protein could be potential biomarkers for rhGH administration. Preliminary assessments of gender, age, acute sport activities and GHRP-2 (pralmorelin, a rhGH releasing peptide) influence suggest they are not relevant confounding factors. Thus, the selected markers present high sensitivity and a larger detection window for rhGH detection than IGF-1 itself. PMID:27243825

  18. The Effects of Different Levels of Dietary Protein and L-Carnitine on Blood Sugar and Lipids of the New GIFT Strain of Juvenile Nile Tilapia (Oreochromis niloticus

    Directory of Open Access Journals (Sweden)

    Gang Chen

    2009-01-01

    Full Text Available The new GIFT (Genetically Improved Farmed Tilapia strain of Nile tilapia is a popular cultivated fish in Asia, but intensive aquaculture using nutritionally imbalanced feed has led to disorder of lipid metabolisms. An 8-week feeding experiment was conducted in order to assess the effects of different levels of L-carnitine (0, 200, 400, 600, and 800 mg/kg and dietary protein (22, 25, and 28% on blood sugar and blood lipid contents of the new juvenile GIFT strain of Nile tilapia. Results showed that dietary protein and L-carnitine had significant influences on glucose (GLU, high-density lipoprotein–cholesterol (HDL-C, total cholesterol (TC, triglyceride (TG, and low-density lipoprotein–cholesterol (LDL-C in the blood serum. The contents of GLU and HDL-C increased with the increases in dietary protein and L-carnitine levels, while the contents of TC, LDL-C, and TG decreased with the increases in dietary protein and L-carnitine levels. The interactive effect of both dietary protein and L-carnitine was most significant on GLU (p = 0.0001, followed by TG (p = 0.001, TC (p = 0.005, HDL-C (p = 0.056, and LDL-C (p = 0.109. These results suggested that high levels of dietary protein and L-carnitine supplementation reduce blood lipids and the burden of the fish liver.

  19. Methoden zur Immobilisierung von Proteinen auf Polyurethan- und Goldoberflächen und ihr Einfluss auf Konformation und Aktivität der Proteine

    OpenAIRE

    Kreider, Alexej

    2014-01-01

    In recent years anti-freeze proteins became the focus of interest for materials science due to their ice-crystall-growth inhibiting properties, recrystallisation properties and ice-crystall structuring properties. The transfer of these properties to surfaces by means of a molecular biomimetic approach is the challenge as well as motivation of this work. Here, the molecular bionic approach is based on chemical immobilization methods of proteins to solid surfaces. Thus, the first question of th...

  20. Qualitative Inhaltsanalyse anhand von Zielen und Problemen von Psychotherapiepatienten

    OpenAIRE

    Dick, Yvonne

    2004-01-01

    Ziel der vorliegenden Arbeit war, das Vorgehen der Qualitativen Inhaltsanalyse anhand von Zielen und Problemen von Psychotherapiepatienten (Klartextangaben)zu beschreiben. Ein weiteres Ziel war es die vorherige Studie bezüglich der Probleme und Ziele von Psychotherapiepatienten zu replizieren, um die Anwendbarkeit des damals erstellten Kategoriensystems zu überprüfen. Gleichzeitig entwickelten wir das bereits existierende Kategoriensystem weiter. Dabei war es uns wichtig, der Vielgestaltigkei...

  1. Enzymkinetik von Phospholipase C und Aggregationsverhalten von Gentransfer-Komplexen

    OpenAIRE

    Galneder, Reinhard Josef

    2007-01-01

    Im Rahmen dieser Arbeit wurde ein Experiment zur Laserfallen-kontrollierten Mikroelektrophorese aufgebaut und getestet, das zeitaufgelöste Messungen des Oberflächenpotentials an einzelnen kolloidalen Teilchen ermöglichte. Mit dieser Methode konnte im folgenden die Enzymkinetik von Phospholipase C mit einer bisher nicht erreichten Zeitauflösung von ca. 1 sec gemessen werden. Dazu wurden Silika-Kugeln mit einem Radius von 500 nm mit einer Lipidmembran aus neutralem Phosphatidylcholin und dem ne...

  2. The use of blood protein polymorphism to estimate genetic distance among populations of Indonesian native sheep, St. Croix and Merino

    Directory of Open Access Journals (Sweden)

    Agus Suparyanto

    2002-03-01

    Full Text Available The genetic distance among populations of Indonesia native sheep (Ciamis, Garut, Sumatera and Garahan, St. Croix and Merino were estimated to investigate the genetic relationship among those breeds. Blood protein polymorphism of transferin (Tf, post-transferin (PTf, albumin (Alb, post-albumin (PAlb were detected from blood plasma, while haemoglobine (Hb was detected from erythrocyte using Polyacrylamide Gel Electrophoresis (PAGE. Results of PAGE showed that Tf was controlled by 6 alleles, while Alb by 4 alleles, PTf by 3 Alleles and PAlb and Hb by 2 alleles. Value of breeding coefficient within individual subpopulations (FIS for Tf (-0,0014, Alb (-0,0046 and Hb (0,0256 were not significantly different by noel. These results show that data of gene frequency are still following Hardy-Weinberg Equilibrium and inbreeding inside the sub population did not occur. The closest distance among the native breeds is the subpopulations of Ciamis and Garut due to neighboring area and similar traits of Thin Tail Sheep. The genetic distance of both population to Sumatera Thin Tail Sheep and Garahan Fat Tail are quite far. In addition to that results all Indonesian native breed were distinctly different from St. Croix and Merino.

  3. Detection of Homocysteine and C-Reactive Protein in the Saliva of Healthy Adults: Comparison with Blood Levels

    Directory of Open Access Journals (Sweden)

    Michael C. Dillon

    2010-07-01

    Full Text Available Inflammation and cardiovascular disease are associated with elevated serum levels of C-Reactive Protein (CRP and homocysteine. The presence of both molecules in saliva provides an opportunity for development of non-invasive assessments of disease risk. However, salivary CRP and homocysteine reference ranges and their correlation with serum levels are unknown. This study investigated if CRP and homocysteine could be routinely detected in the saliva of healthy adults and the relationship between salivary and blood levels. CRP and homocysteine concentrations were determined using ELISA and enzymatic assays respectively. Homocysteine was detected in only two saliva samples (n = 55. CRP was measurable in all saliva samples (range: 0.05 to 64.3 µg/L; median = 1.2 µg/L and plasma samples (range: 0.14 to 31.1 mg/L; median = 2.0 mg/L. Regression analysis demonstrated no relation- ship between CRP concentration in saliva and plasma (R2 = 0.001. Generalized linear models including variables such as saliva flow rate and time since eating or drinking also did not pass lack of fit testing. Therefore, a relationship between CRP concentration in saliva and blood could not be established in this group of subjects. More sensitive detection methods are needed to determine if a correlation between salivary and serum homocysteine levels exists.

  4. Studies of blood groups and protein polymorphisms in the Brazilian horse breeds Mangalarga Marchador and Mangalarga (Equus caballus

    Directory of Open Access Journals (Sweden)

    Andréia Samaha Lippi

    2003-12-01

    Full Text Available Allelic frequencies at 12 loci (five blood groups: C, D, K, P, and U; and seven protein polymorphisms: Al, A1B, Es, Gc, Hb, PGD, and Tf, are given for two Brazilian horse breeds: Mangalarga Marchador and Mangalarga. The high genetic identity value found (96.0% is consistent with their common origin, although, at some point of the development of Mangalarga Marchador, Mangalarga separated from the original stock. The expected average heterozygosity was higher in Mangalarga Marchador. The populations presented genetic differentiation, as shown by the statistically significant value of F ST. The nonsignificant F IS values showed that there was no appreciable consanguineous mating in any of the two populations. Exclusion probability calculated for the 12 loci was 87.0% and 86.5% for Mangalarga Marchador and Mangalarga, respectively. No genetic equilibrium was observed in the A1B, Tf, and Es loci of Mangalarga Marchador. The frequencies of blood factors A, Q, and T were calculated.

  5. Effect of whey protein on blood lipid profiles: a meta-analysis of randomized controlled trials.

    Science.gov (United States)

    Zhang, J-W; Tong, X; Wan, Z; Wang, Y; Qin, L-Q; Szeto, I M Y

    2016-08-01

    Previous studies have suggested that whey supplementation may have beneficial effects on lipid profiles, although results were inconsistent. A literature search was performed in March 2015 for randomized controlled trials observing the effects of whey protein and its derivatives on circulating levels of triacylglycerol (TG), total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C) and high-density lipoprotein cholesterol (HDL-C). A meta-analysis was subsequently conducted. The meta-analysis results of 13 trials showed that whey supplementation significantly reduced the circulating TG level by 0.11 mmol/l (95% CI: -0.21, 0 mmol/l), whereas the whey protein had no effects on circulating TC (-0.11 mmol/l, 95% CI: -0.27, 0.05 mmol/l), LDL-C (-0.08 mmol/l, 95% CI: -0.23, 0.07 mmol/l) and HDL-C (0.01 mmol/l, 95% CI: -0.04, 0.05 mmol/l). Subgroup analysis showed that significant TG reduction disappeared in participants with low body mass index, low supplemental whey dose or under exercise training/energy restriction during the trial. No evidence of heterogeneity across studies and publication bias was observed. In conclusion, our findings demonstrated that the effects of whey protein supplementation were modest, with an overall lowering effect on TG but no effect on TC, LDL-C and HDL-C. PMID:27026427

  6. [The response of the blood proteins to ablation of the capsaicin-sensitive nerves].

    Science.gov (United States)

    Kostina, N E; Spiridonov, V K

    2005-07-01

    Effects of neurotoxic doses of capsaicin (150 mg/kg) on the protein content in electrophoretic fractions (PAAG) in the Wistar rat plasma were studied. In early period (7 days) after administration of capsaicin, an increase of the alpha1-, alpha2-globulins and a decrease of the albumin, gamma-globulins, were observed. After 14-30 days, increase of the albumin and decrease of the alpha1-, gammay-globulins were detected. The ablation of the capsaicin-sensitive nerves abrogated the changes of positive and negative acute phase reactants induced by zymosan and diminished the content of gamma-globulins. PMID:16206619

  7. C-reactive protein is produced by a small number of normal human peripheral blood lymphocytes

    OpenAIRE

    1986-01-01

    Biosynthetic labeling with [35S]met and immunoprecipitation with anti-C- reactive protein (CRP) antibodies and Staphylococcus aureus indicate that cell surface CRP is produced by lymphocytes. The ability of anti- CRP to reduce NK activity, and the demonstration that 125I-anti-CRP- labeled PBL are found in low-density Percoll fractions associated with large granular lymphocyte (LGL) and NK activity suggest that S-CRP- bearing cells are NK effectors. The production of S-CRP by LGL supports this...

  8. Relationship between blood urea, protein, creatinine, triglycerides and macro-mineral concentrations with the quality and quantity of milk in dairy Holstein cows

    OpenAIRE

    Azadeh Babapour; Siamak Asri-Rezaei; Gholamali Moghadam; Ali-Gholi Ramin; Shahram Nozad; Sina Ramin

    2012-01-01

    Seventy six high and low producer cows were selected to determine the composition of the blood and milk parameters, and their interrelationships to determine the indices which could be useful to improve the milk yield. The highest mean blood concentrations were found in high producer cows. Mean values for blood urea nitrogen (BUN), serum protein (SPtn), creatinine, triglycerides (TGs), cholesterol, and beta-hydroxybutyric acid (BHB) were 25.10 mg dL-1, 10.15 g dL-1, 0.81, 62.30, 177.10 and 0....

  9. Herstellung von Chitosan und einige Anwendungen

    Science.gov (United States)

    Struszczyk, Marcin Henryk

    2001-05-01

    . Die aus Chitosan-Dispersionen hergestellten MCChB-Filme weisen bessere mechanische Eigenschaften (Bruchfestigkeit, Dehnung) und eine höhere Wasseraufnahmefähigkeit auf als Filme, die nach herkömmlichen Methoden aus sauerer Lösung hergestellt werden. Die Einführung von Proteinen ändert die mechanischen Eigenschaften der MCChB-Filme abhängig von der Art, der Proteine sowie des DD und der Mv des eingesetzte Chitosan. Die Zugabe von Protein beschleunigt den biologischen Abbau der MCChB-Filme. Aus den untersuchten MCChB-Filmen mit Proteinzusatz können leichte, reißfeste und dennoch elastische Materialen hergestellt werden. 4. Mit Hilfe von MCChB-Dispersion kann Papier modifiziert werden. Dadurch werden die mechanischen Eigenschaften verbessert und die Wasseraufnahme wird verringert. Die Zugabe von Proteinen verringert das Wasseraufnahmevermögen noch weiter. Ein geringes Wasseraufnahmevermögen ist der bedeutendste Faktor bei der Papierherstellung. Auch Papier, das mit einem MCChB-Protein-Komplexe modifiziert wurde, zeigt gute mechanische Eigenschaften. 5. Wird Chitosan durch unmittelbare Einführung von MCChB auf Cellulose-Fasern aufgebracht, so erhält man eine netzartige Struktur, während durch Ausfällung aufgebrachtes Chitosan eine dünne Schicht auf den Cellulose-Fasern bildet. Die netzartige Struktur erleichtert die Bioabbaubarkeit, während die Schichtstruktur diese erschwert. 6. Die guten mechanischen Eigenschaften, die geringe Wasseraufnahmefähigkeit und die mit Cellulose vergleichbare Bioabbaubarkeit von Papier, das mit MCChB modifiziert wurde, lassen MCChB für die Veredlung von Papier nützlich erscheinen. 1. Deacetylation of the crustacean chitosan causes drastically decrease in the Mv with increasing reaction temperature and time as well as the concentration of sodium hydroxide. However, the DD are relatively less affected. Pandalus borealis is a good source for production of chitosan having high Mv and low DD, whereas chitosan of medium to low Mv

  10. Imaging of Von Meyenburg complexes.

    Science.gov (United States)

    Pech, L; Favelier, S; Falcoz, M T; Loffroy, R; Krause, D; Cercueil, J P

    2016-04-01

    Von Meyenburg complexes, or biliary hamartomas, are frequently incidentally detected. They are usually easy to characterize with magnetic resonance imaging. However, in some occasions they are difficult to differentiate from other liver lesions, in particular from small liver metastases. Von Meyenburg complexes are developmental malformations of the ductal plate. They can be found in association with Caroli disease and Caroli syndrome. Like other ductal plate malformations, Von Meyenburg complexes associated with cholangiocarcinoma have been described and their relationship has been established. This review provides an update on the etiopathogenesis of Von Meyenburg complexes, illustrates the imaging features on ultrasound, CT and MRI of this condition and discusses the most common diagnostic pitfalls. The relationships between Von Meyenburg complexes and the various ductal plate malformations and the most recent literature data regarding the relationships between Von Meyenburg complexes and cholangiocarcinoma are presented. PMID:26522945

  11. Role of fluid shear stress in regulating VWF structure, function and related blood disorders.

    Science.gov (United States)

    Gogia, Shobhit; Neelamegham, Sriram

    2015-01-01

    Von Willebrand factor (VWF) is the largest glycoprotein in blood. It plays a crucial role in primary hemostasis via its binding interaction with platelet and endothelial cell surface receptors, other blood proteins and extra-cellular matrix components. This protein is found as a series of repeat units that are disulfide bonded to form multimeric structures. Once in blood, the protein multimer distribution is dynamically regulated by fluid shear stress which has two opposing effects: it promotes the aggregation or self-association of multiple VWF units, and it simultaneously reduces multimer size by facilitating the force-dependent cleavage of the protein by various proteases, most notably ADAMTS13 (a disintegrin and metalloprotease with thrombospondin type repeats, motif 1 type 13). In addition to these effects, fluid shear also controls the solution and substrate-immobilized structure of VWF, the nature of contact between blood platelets and substrates, and the biomechanics of the GpIbα-VWF bond. These features together regulate different physiological and pathological processes including normal hemostasis, arterial and venous thrombosis, von Willebrand disease, thrombotic thrombocytopenic purpura and acquired von Willebrand syndrome. This article discusses current knowledge of VWF structure-function relationships with emphasis on the effects of hydrodynamic shear, including rapid methods to estimate the nature and magnitude of these forces in selected conditions. It shows that observations made by many investigators using solution and substrate-based shearing devices can be reconciled upon considering the physical size of VWF and the applied mechanical force in these different geometries. PMID:26600266

  12. Transporter protein and drug-conjugated gold nanoparticles capable of bypassing the blood-brain barrier.

    Science.gov (United States)

    Zhang, Yanhua; Walker, Janelle Buttry; Minic, Zeljka; Liu, Fangchao; Goshgarian, Harry; Mao, Guangzhao

    2016-01-01

    Drug delivery to the central nervous system (CNS) is challenging due to the inability of many drugs to cross the blood-brain barrier (BBB). Here, we show that wheat germ agglutinin horse radish peroxidase (WGA-HRP) chemically conjugated to gold nanoparticles (AuNPs) can be transported to the spinal cord and brainstem following intramuscular injection into the diaphragm of rats. We synthesized and determined the size and chemical composition of a three-part nanoconjugate consisting of WGA-HRP, AuNPs, and drugs for the treatment of diaphragm paralysis associated with high cervical spinal cord injury (SCI). Upon injection into the diaphragm muscle of rats, we show that the nanoconjugate is capable of delivering the drug at a much lower dose than the unconjugated drug injected systemically to effectively induce respiratory recovery in rats following SCI. This study not only demonstrates a promising strategy to deliver drugs to the CNS bypassing the BBB but also contributes a potential nanotherapy for the treatment of respiratory muscle paralysis resulted from cervical SCI. PMID:27180729

  13. Plasma protein induced clustering of red blood cells in micro capillaries

    Science.gov (United States)

    Wagner, Christian; Brust, Mathias; Aouane, Othmane; Flormann, Daniel; Thiebaud, Marine; Verdier, Claude; Coupier, Gwennou; Podgorski, Thomas; Misbah, Chaouqi; Selmi, Hassib

    2013-11-01

    The plasma molecule fibrinogen induces aggregation of RBCs to clusters, the so called rouleaux. Higher shear rates in bulk flow can break them up which results in the pronounced shear thinning of blood. This led to the assumption that rouleaux formation does not take place in the microcapillaries of the vascular network where high shear rates are present. However, the question is of high medical relevance. Cardio vascular disorders are still the main cause of death in the western world and cardiac patients have often higher fibrinogen level. We performed AFM based single cell force spectroscopy to determine the work of separation. Measurements at low hematocrit in a microfluidic channel show that the number of size of clusters is determined by the adhesion strength and we found that cluster formation is strongly enhanced by fibrinogen at physiological concentrations, even at shear rate as high as 1000 1/s. Numerical simulations based on a boundary integral method confirm our findings and the clustering transition takes place both in the experiments and in the simulations at the same interaction energies. In vivo measurements with intravital fluorescence microscopy in a dorsal skin fold chamber in a mouse reveal that RBCs indeed form clusters in the micrcapillary flow. This work was supported by the German Science Foundation research imitative SFB1027.

  14. Semantische Adaption von Komponenten

    OpenAIRE

    Söldner, Guido

    2012-01-01

    Im Bereich der Informationstechnik ist die Adaption von Software an veränderte Gegebenheiten und Umgebungen ein wichtiges Thema. Dabei bestimmen vor allem die konkret verwendete Software-Programmiersprache, die benutzten Entwurfsmuster und die der Applikation zugrundeliegende Software-Architektur, mit welchem Aufwand eine Anwendung auf neue Anforderungen eingehen kann. Darüber hinaus war und ist es interessant, Mechanismen zu entwickeln, mit denen Applikationen selbst auf spontane Änderungen ...

  15. Molekulare Mechanismen von Pankreaserkrankungen

    OpenAIRE

    Ockenga, Johann

    2003-01-01

    Die Ätiologie von entzündlichen Pankreaserkrankungen, insbesondere bei den idiopathischen Pankreatitiden, ist weitgehend noch nicht verstanden. In der folgenden Arbeit sollen immunologische und molekularbiologische Aspekte zu Pankreaserkrankungen unter Berücksichtigung eigener Untersuchungen dargestellt werden. Zu Beginn unserer Arbeit haben wir untersucht inwieweit immunologische Veränderungen an der Entstehung einer chronischen Pankreatitis beteiligt sind. Wir fanden eine systemische Akti...

  16. Positive Aspekte von Erosionsprozessen

    OpenAIRE

    Haffner, Willibald

    1995-01-01

    Negative Aspekte von Erosion, insbesondere der Bodenerosion, sind in der Fachliteratur vielseitig dokumentiert. Dieser Aufsatz geht der Frage nach, welche positiven Aspekte erosiven Sediment- und Bodenverlagerungen abzugewinnen sind. Folgende Beispiele aus Mitteleuropa, Nepal und dem Jemen werden diskutiert: - Entstehung edaphischer Gunsträume durch Wasser- und Winderosion - Bodenabtrag im Muschelkalk: Auf- oder Abwertung des erodierten Standortes? - Erschließung kalk- und nährstoffr...

  17. Von Kursachsen nach Europa

    OpenAIRE

    Link, Ivonne; Wiegand, Peter

    2011-01-01

    Innovativer Zugriff auf raumbezogene grafische Information – unter diesem Titel erfolgte zwischen 2009 und 2011 der Ausbau des umfangreichen Rechercheportals für kartografische Fachinformationen („Kartenforum“) der Sächsischen Landesbibliothek – Staats- und Universitätsbibliothek Dresden (SLUB). Im Rahmen des von der Deutschen Forschungsgemeinschaft geförderten Projektes wurden weitere 12.000 historische Karten und Ansichten hoch aufgelöst digitalisiert, katalogisiert sowie sachlich erschloss...

  18. Nutzerorientiertes Management von materiellen und immateriellen Informationsobjekten

    OpenAIRE

    Hübsch, Chris

    2001-01-01

    Schaffung einer stabilen, erweiterbaren und skalierbaren Infrastruktur für die Bereitstellung von Diensten im Umfeld von Bibliotheken und ähnlichen wissensanbietenden Einrichtungen unter Verwendung von XML-RPC und Python.

  19. Wine grape pomace flour improves blood pressure, fasting glucose and protein damage in humans: a randomized controlled trial

    Directory of Open Access Journals (Sweden)

    Inés Urquiaga

    2015-01-01

    Full Text Available BACKGROUND: The Mediterranean diet is a healthy diet with positive scientific evidence of preventing chronic diseases. Bioactive components support the healthy properties of the Mediterranean diet. Antioxidants and fiber, two components of the Mediterranean diet, are key functional nutrients for healthy eating and nutrition. Wine grape pomace is a rich source of these dietary constituents and may be beneficial for human health. Our hypothesis was that the intake of red wine grape pomace flour (WGPF prepared from red wine grapes (Cabernet Sauvignon variety reduced the metabolic syndrome in humans. To evaluate the effect of WGPF on components of metabolic syndrome we design a 16-week longitudinal intervention study. Thirty-eight males, 30-65 years of age, with at least one component of metabolic syndrome, were randomly assigned to either the intervention group (n = 25 or the control group (n = 13. At lunch, the intervention group was given 20 g of WGPF per day, which contained 10 g of dietary fiber, 822 mg of polyphenols and an antioxidant capacity of 7258 ORAC units. Both groups were asked to maintain their regular eating habits and lifestyles. Clinical evaluation, anthropometric measurements and biochemical blood analyses were done at the beginning and the end of the study. RESULTS: WGPF intake significantly decreased systolic and diastolic blood pressure as well as fasting glucose levels. Plasma γ-tocopherol and δ-tocopherol increased and carbonyl group in plasma protein decreased in WGPT group, significantly. No significant effect was observed for waist circumference, HDL cholesterol, triglycerides, total antioxidant capacity and vitamin C in and between groups. The group-dependent magnitude of the differences between the baseline and final postprandial insulin values and γ-tocopherol concentrations was statistically significant. CONCLUSIONS: The consumption of WGPF-rich in fiber and polyphenol antioxidants, as a food supplement in a

  20. Dichteoptimierung und Strukturanalyse von Hartkugelpackungen

    OpenAIRE

    Lochmann, Kristin

    2010-01-01

    Bei der Verwendung von Hartkugelpackungen als Modelle für verschiedene Systeme in Physik, Chemie und den Ingenieurwissenschaften kommen einige Fragen auf, z.B. nach dem Zusammenhang zwischen der Packungsdichte und der Radienverteilung der Kugeln bzw. der Packungsstruktur. Der erste Teil dieser Arbeit beschäftigt sich mit dem Problem der optimalen Packungsdichte von zufällig dichten Packungen. Es wird ein Optimierungsalgorithmus vorgestellt, der aus einer vorgegebenen Klasse von Radienverteilu...

  1. Tragverhalten von Textilbeton mit Kurzfasern

    OpenAIRE

    Brameshuber, Wolfgang; Hinzen, Marcus

    2011-01-01

    Das Tragverhalten von Textilbeton kann durch den Einsatz von Kurzfasern erheblich verbessert werden. Untersuchungen zeigen eine Anhebung der Erstrisslast, ein dehnungsverfestigendes Verhalten, eine Verfeinerung des Rissbildes sowie eine Anhebung der maximalen Tragfähigkeit. Unklar sind bisher noch die genauen Mechanismen, die eine gezielte Einstellung des Tragverhaltens ermöglichen. Der Beitrag fasst die Untersuchungen zu den einzelnen Bereichen der Spannungs- ehnungslinie von Textilbeton mit...

  2. Identification of multiple novel protein biomarkers shed by human serous ovarian tumors into the blood of immunocompromised mice and verified in patient sera.

    Directory of Open Access Journals (Sweden)

    Lynn A Beer

    Full Text Available The most cancer-specific biomarkers in blood are likely to be proteins shed directly by the tumor rather than less specific inflammatory or other host responses. The use of xenograft mouse models together with in-depth proteome analysis for identification of human proteins in the mouse blood is an under-utilized strategy that can clearly identify proteins shed by the tumor. In the current study, 268 human proteins shed into mouse blood from human OVCAR-3 serous tumors were identified based upon human vs. mouse species differences using a four-dimensional plasma proteome fractionation strategy. A multi-step prioritization and verification strategy was subsequently developed to efficiently select some of the most promising biomarkers from this large number of candidates. A key step was parallel analysis of human proteins detected in the tumor supernatant, because substantially greater sequence coverage for many of the human proteins initially detected in the xenograft mouse plasma confirmed assignments as tumor-derived human proteins. Verification of candidate biomarkers in patient sera was facilitated by in-depth, label-free quantitative comparisons of serum pools from patients with ovarian cancer and benign ovarian tumors. The only proteins that advanced to multiple reaction monitoring (MRM assay development were those that exhibited increases in ovarian cancer patients compared with benign tumor controls. MRM assays were facilely developed for all 11 novel biomarker candidates selected by this process and analysis of larger pools of patient sera suggested that all 11 proteins are promising candidate biomarkers that should be further evaluated on individual patient blood samples.

  3. Untersuchung von Metallionen induzierten Konformationsänderungen in Proteinkinasen mittels Surface Plasmon Resonance

    OpenAIRE

    Zimmermann, Bastian; Herberg, Friedrich

    2001-01-01

    Surface Plasmon Resonance (SPR) kann benutzt werden, um Protein/Protein Wechselwirkungen aber auch Nukleotid/Protein-Wechselwirkungen kinetisch genau zu charakterisieren. Unser Modellsystem für Proteinkinasefunktion ist die cAMP-abhängige Proteinkinase (cAPK), die das g-Phosphat von ATP auf Serin oder Threoninreste von Substratmolekülen überträgt. Zwei Typen von Inhibitoren der katalytischen Untereinheit (C) der cAPK sind bekannt: die regulatorischen Untereinheiten Typ I und Typ II und die so...

  4. Comparison Of Blood Proteins And Some Hormonal Levels In Pregnant And Non-Pregnant Cows

    International Nuclear Information System (INIS)

    The aim of this study is to determine the changes in serum protein and its fractions by using electrophoresis in Holstein cows during different months of pregnancy in comparison with non-pregnant cows and to determine hormonal levels including T4, T3 and progesterone hormones. The samples were taken from 40 pregnant cows during deferent months and 10 non-pregnant cows. Significant decrease in the levels of total protein, albumin and globulin were observed in the third and late month of pregnancy than in mid pregnancy where the values were 6.5, 3.1 and 3.4 g/dl for early months and 6.5, 3.2 and 3.3 g/dl for late month as compared to the non-pregnant cows. Significant increase in α-1globulin was observed during months of pregnancy by about 33.3%. The decrease in the levels of α-2, β and γ-globulins were recorded by about 10%, 45.3% and 21.6%, respectively. A marked decrease in T4 hormone (5.0 μg/dl) was observed in pregnant cows than in non-pregnant ones (7.1 μg/dl). Also, a decreasing T3 level (169 ng/dl) was recorded as compared to non-pregnant cows (221 ng/dl). High significant increase in progesterone level was recorded in the mid pregnancy until reached the maximum value (49.94 ng/ml) at the 7th month of pregnancy then declined (2.42 ng/ml) at the late month of pregnancy. In conclusion, during pregnancy of Holstein dairy cows, a decline in protein fractions and thyroid hormonal levels were recorded during different months as compared to non- pregnant cows. The opposite trend was observed in progesterone levels. The increasing progesterone level at the mid pregnancy indicated its importance in the continuation of pregnancy and maintenance of fetus against maternal rejection.

  5. Phasenverhalten von Polyethylen in Mischsystemen

    OpenAIRE

    Schnell, Matthias

    2003-01-01

    Die vorliegende Arbeit beschäftigt sich mit dem Phasenverhalten von Polyethylen (PE) in nicht-reaktiven und in reaktiven Systemen. Von drei eng verteilten Polyethylenen (Mw = 6,4, 82 bzw. 380 kg/mol) in n-Hexan sowie für das System 2,2-Dimethylbutan / PE 82 wurde die Entmischung in Abhängigkeit von der Zusammensetzung, dem Druck und der Temperatur experimentell bestimmt. Die Modellierung der Trübungskurven erfolgte nach der Theorie von Sanchez und Lacombe. Dieser Ansatz beschreibt die Ergebni...

  6. Neurogenically mediated leakage of plasma protein occurs from blood vessels in dura mater but not brain

    International Nuclear Information System (INIS)

    Utilizing 125I-BSA administered intravenously, a simple, reliable, and sensitive method was established for the detection of plasma protein extravasation in the dura of rats and guinea pigs following chemical, electrical, or immunological stimulation. Extravasated 125I-BSA or Evans blue was noted in the dura and conjunctiva but not in the temporalis muscle of saline-perfused rats following intravenous capsaicin, 1 mumol/kg. Capsaicin-induced extravasation was mediated by unmyelinated and small myelinated fibers since leakage did not develop in adult animals in whom these fibers were destroyed by capsaicin pretreatment (50 mg/kg) as neonates. An ipsilateral increase in Evans blue and 125I-BSA was found in the dura, eyelids, lips and gingival mucosa, and snout following electrical stimulation of the rat trigeminal ganglion. This increase was also C-fiber dependent. Among those peptides contained in perivascular afferent fibers and administered intravenously, substance P (SP) and neurokinin A (NKA), but not calcitonin gene-related peptide, caused a dose-dependent extravasation in the dura and conjunctiva of rats. Neonatal capsaicin pretreatment did not attenuate SP- nor NKA-induced effects in the dura and actually increased extravasation in the conjunctiva. Intravenous administration of 5-HT or bradykinin to normal adult rats or adult rats pretreated as neonates with capsaicin increased levels of 125I-BSA in both the dura and the conjunctiva. Histamine and prostaglandin E2, on the other hand, caused protein leakage in the conjunctiva but not in the dura of rats; however, histamine did induce extravasation in the dura of guinea pigs

  7. Neurogenically mediated leakage of plasma protein occurs from blood vessels in dura mater but not brain

    Energy Technology Data Exchange (ETDEWEB)

    Markowitz, S.; Saito, K.; Moskowitz, M.A.

    1987-12-01

    Utilizing /sup 125/I-BSA administered intravenously, a simple, reliable, and sensitive method was established for the detection of plasma protein extravasation in the dura of rats and guinea pigs following chemical, electrical, or immunological stimulation. Extravasated /sup 125/I-BSA or Evans blue was noted in the dura and conjunctiva but not in the temporalis muscle of saline-perfused rats following intravenous capsaicin, 1 mumol/kg. Capsaicin-induced extravasation was mediated by unmyelinated and small myelinated fibers since leakage did not develop in adult animals in whom these fibers were destroyed by capsaicin pretreatment (50 mg/kg) as neonates. An ipsilateral increase in Evans blue and /sup 125/I-BSA was found in the dura, eyelids, lips and gingival mucosa, and snout following electrical stimulation of the rat trigeminal ganglion. This increase was also C-fiber dependent. Among those peptides contained in perivascular afferent fibers and administered intravenously, substance P (SP) and neurokinin A (NKA), but not calcitonin gene-related peptide, caused a dose-dependent extravasation in the dura and conjunctiva of rats. Neonatal capsaicin pretreatment did not attenuate SP- nor NKA-induced effects in the dura and actually increased extravasation in the conjunctiva. Intravenous administration of 5-HT or bradykinin to normal adult rats or adult rats pretreated as neonates with capsaicin increased levels of /sup 125/I-BSA in both the dura and the conjunctiva. Histamine and prostaglandin E2, on the other hand, caused protein leakage in the conjunctiva but not in the dura of rats; however, histamine did induce extravasation in the dura of guinea pigs.

  8. Blood-Brain Barrier and Breast Cancer Resistance Protein: A Limit to the Therapy of CNS Tumors and Neurodegenerative Diseases

    Science.gov (United States)

    Iorio, Anna Lisa; da Ros, Martina; Fantappiè, Ornella; Lucchesi, Maurizio; Facchini, Ludovica; Stival, Alessia; Becciani, Sabrina; Guidi, Milena; Favre, Claudio; de Martino, Maurizio; Genitori, Lorenzo; Sardi, Iacopo

    2016-01-01

    The treatment of brain tumors and neurodegenerative diseases, represents an ongoing challenge. In Central Nervous System (CNS) the achievement of therapeutic concentration of chemical agents is complicated by the presence of distinct set of efflux proteins, such as ATP-Binding Cassette (ABC) transporters localized on the Blood-Brain Barrier (BBB). The activity of ABC transporters seems to be a common mechanism that underlies the poor response of CNS diseases to therapies. The molecular characterization of Breast Cancer Resistance Protein (BCRP/ABCG2), as an ABC transporter conferring multidrug resistance (MDR), has stimulated many studies to investigate its activity on the BBB, its involvement in physiology and CNS diseases and its role in limiting the delivery of drugs in CNS. In this review, we highlight the activity and localization of BCRP on the BBB and the action that this efflux pump has on many conventional drugs or latest generation molecules used for the treatment of CNS tumors and other neurodegenerative diseases. PMID:26584727

  9. Pf155/RESA protein influences the dynamic microcirculatory behavior of ring-stage Plasmodium falciparum infected red blood cells

    Science.gov (United States)

    Diez-Silva, Monica; Park, Yongkeun; Huang, Sha; Bow, Hansen; Mercereau-Puijalon, Odile; Deplaine, Guillaume; Lavazec, Catherine; Perrot, Sylvie; Bonnefoy, Serge; Feld, Michael S.; Han, Jongyoon; Dao, Ming; Suresh, Subra

    2012-08-01

    Proteins exported by Plasmodium falciparum to the red blood cell (RBC) membrane modify the structural properties of the parasitized RBC (Pf-RBC). Although quasi-static single cell assays show reduced ring-stage Pf-RBCs deformability, the parameters influencing their microcirculatory behavior remain unexplored. Here, we study the dynamic properties of ring-stage Pf-RBCs and the role of the parasite protein Pf155/Ring-Infected Erythrocyte Surface Antigen (RESA). Diffraction phase microscopy revealed RESA-driven decreased Pf-RBCs membrane fluctuations. Microfluidic experiments showed a RESA-dependent reduction in the Pf-RBCs transit velocity, which was potentiated at febrile temperature. In a microspheres filtration system, incubation at febrile temperature impaired traversal of RESA-expressing Pf-RBCs. These results show that RESA influences ring-stage Pf-RBCs microcirculation, an effect that is fever-enhanced. This is the first identification of a parasite factor influencing the dynamic circulation of young asexual Pf-RBCs in physiologically relevant conditions, offering novel possibilities for interventions to reduce parasite survival and pathogenesis in its human host.

  10. Evaluation of C-reactive protein and interleukin-6 in the peripheral blood of patients with chronic periodontitis

    Directory of Open Access Journals (Sweden)

    Gani Dhruva

    2009-01-01

    Full Text Available Aims and Objectives: The aim of the present study was to investigate systemic levels of inflammatory markers of cardiovascular diseases like C-reactive protein and interleukin-6 in patients with chronic periodontitis, in comparison to periodontally healthy individuals. Materials and Methods: A total of 42 individuals, both males and females above the age of 30 years, were included in the study. Healthy controls (Group I, n = 14, chronic localized periodontitis (Group II, n = 14, and chronic generalized periodontitis (Group III, n = 14, all without any medical disorder, were recruited. Peripheral blood samples were taken and C-reactive protein (CRP levels were estimated in the serum samples by using the Particle-Enhanced Turbidimetric Immunoassay (PETIA technique. Serum samples of Interleukin-6 (IL-6 were assayed by using the Chemiluminescent Immunoassay (IMMULITE technique. Results: When mean CRP levels were compared between the groups, group III showed statistical significance when compared to group I (P = 0.04. Group III had a higher median IL-6 level (6.35 pg/mL than Group II (< 5.0 pg/ mL and group I (< 5.0 pg/mL. Differences in median values of IL-6 were not statistically significant in any group (P = 0.29. Conclusion: Periodontitis results in higher systemic levels of CRP and IL-6. These elevated inflammatory factors may increase inflammatory activity in atherosclerotic lesions and potentially increasing the risk for cardiovascular events.

  11. Expression of the antiviral protein Mx in peripheral blood mononuclear cells of pregnant and bred, non-pregnant ewes.

    Science.gov (United States)

    Yankey, S J; Hicks, B A; Carnahan, K G; Assiri, A M; Sinor, S J; Kodali, K; Stellflug, J N; Stellflug, J N; Ott, T L

    2001-08-01

    Interferon-tau (IFN tau) acts locally on the endometrium to suppress estrogen and oxytocin receptor expression and block luteolysis in ruminants. Systemic administration of conceptus homogenates or recombinant ovine IFN tau does not block luteolysis or enhance pregnancy rates in sheep or cattle, respectively. However, IFN tau up-regulates expression of the antiviral protein Mx throughout the entire uterine wall during early pregnancy. These studies determined if conceptus-derived IFN tau also up-regulates Mx expression in components of the circulating immune system that migrate through the endometrial wall. In experiment one, peripheral blood mononuclear cells (PBMC) were isolated from ewes at D26 post-artificial insemination (AI) and Mx mRNA levels examined by Northern and slot-blot hybridization. Pregnancy resulted in a two-fold increase in Mx mRNA levels compared to bred, non-pregnant ewes at D26. In experiment two, PBMC were isolated from ewes at AI, and every three days from D9 to D30. Results showed a four-fold increase in Mx mRNA levels in PBMC from pregnant versus bred, non-pregnant ewes at D15. Increased Mx mRNA, which remained elevated through D30, was accompanied by increased levels of Mx protein. These results show that pregnancy recognition signaling rapidly induces Mx gene expression in PBMC, and are the first to suggest that IFN tau activates gene expression in components of the circulating immune system. PMID:11479146

  12. Structure and Ligand-Binding Mechanism of a Cysteinyl Leukotriene-Binding Protein from a Blood-Feeding Disease Vector.

    Science.gov (United States)

    Jablonka, Willy; Pham, Van; Nardone, Glenn; Gittis, Apostolos; Silva-Cardoso, Lívia; Atella, Georgia C; Ribeiro, José M C; Andersen, John F

    2016-07-15

    Blood-feeding disease vectors mitigate the negative effects of hemostasis and inflammation through the binding of small-molecule agonists of these processes by salivary proteins. In this study, a lipocalin protein family member (LTBP1) from the saliva of Rhodnius prolixus, a vector of the pathogen Trypanosoma cruzi, is shown to sequester cysteinyl leukotrienes during feeding to inhibit immediate inflammatory responses. Calorimetric binding experiments showed that LTBP1 binds leukotrienes C4 (LTC4), D4 (LTD4), and E4 (LTE4) but not biogenic amines, adenosine diphosphate, or other eicosanoid compounds. Crystal structures of ligand-free LTBP1 and its complexes with LTC4 and LTD4 reveal a conformational change during binding that brings Tyr114 into close contact with the ligand. LTC4 is cleaved in the complex, leaving free glutathione and a C20 fatty acid. Chromatographic analysis of bound ligands showed only intact LTC4, suggesting that cleavage could be radiation-mediated. PMID:27124118

  13. Gegenseitige Beeinflussung von Mizellaren Strukturen und Photodimerisierung von Cumarinderivaten

    OpenAIRE

    Yu, Xiuling

    2004-01-01

    In dieser Arbeit wurde die gegenseitige Beeinflussung von mizellaren Strukturen und Photodimerisierung von Cumarinderivaten untersucht. Als Mizellbildner wurden das kationische Cetyltrimethylammoniumbromid (CTAB) und das nicht-ionische Triton X-100, als Solubilisate Cumarinderivaten verwendet. Es wurden rheologische, thermodynamische und photochemische Untersuchungen durchgeführt. Die Viskosität der beiden Tensidsysteme steigt mit zunehmender Solubilisatkonzentration je nach Cumarinderivat un...

  14. Massenspektrometrische Untersuchung von Membranproteinen durch den Einsatz weniger spezifischer Proteasen

    OpenAIRE

    Rietschel, Benjamin

    2010-01-01

    Diese Dissertation befasst sich mit der massenspektrometrischen Analytik von Membranproteinen. Diese gestaltet sich aufgrund der hydrophoben Natur der Proteine und der damit verbundenen schlechten Löslichkeit in wässrigen Puffersystemen als deutlich schwieriger als die Untersuchung löslicher Proteine. Einige weitere Probleme entstehen durch die konsequente Verwendung der Referenzprotease Trypsin zur Hydrolyse der Proteine. Sie spaltet ausschließlich Peptidbindungen nach basischen Aminosäuren,...

  15. 15N-enrichments of ammonia and glutamine in blood after infusion of 15N-ammonia in chickens fed low or high protein diet

    International Nuclear Information System (INIS)

    In this experment, the blood ammonia and glutamine amide came from infused ammonia were determined when N-15 labeled ammonium acetate was intraportally infused into the chickens fed 5 or 20 % protein diet. The data obtained indicated that the infused ammonia was taken into blood glutamine amide, and also accumulated in blood as it is, in both dietary groups. 10 to 12 months old White Leghorn male birds were used. The experimental diet was fed once a day for 5 days to the birds weighting about 1.2 kg by 35 g per kg body weight. The experimental diet was consumed within 40 min in all cases. Cardiac and portal catheterization were performed for blood collection and ammonia infusion, respectively. After finishing the infusion, blood samples were taken to analyze the ammonia and glutamine contents and their N-15 enrichment. Statistical difference was not observed in the appearance of N-15 in ammonia and glutamine amide between two dietary groups. The N-15 enrichment in blood ammonia and the amide of plasma glutamine, and the calculated exogenous nitrogen in the ammonia and glutamine amide tended to be more in the 5 % protein diet group than the other. (Kako, I.)

  16. Structure and ligand-binding properties of the biogenic amine-binding protein from the saliva of a blood-feeding insect vector of Trypanosoma cruzi

    International Nuclear Information System (INIS)

    Biogenic amine-binding proteins mediate the anti-inflammatory and antihemostatic activities of blood-feeding insect saliva. The structure of the amine-binding protein from R. prolixus reveals the interaction of biogenic amine ligands with the protein. Proteins that bind small-molecule mediators of inflammation and hemostasis are essential for blood-feeding by arthropod vectors of infectious disease. In ticks and triatomine insects, the lipocalin protein family is greatly expanded and members have been shown to bind biogenic amines, eicosanoids and ADP. These compounds are potent mediators of platelet activation, inflammation and vascular tone. In this paper, the structure of the amine-binding protein (ABP) from Rhodnius prolixus, a vector of the trypanosome that causes Chagas disease, is described. ABP binds the biogenic amines serotonin and norepinephrine with high affinity. A complex with tryptamine shows the presence of a binding site for a single ligand molecule in the central cavity of the β-barrel structure. The cavity contains significant additional volume, suggesting that this protein may have evolved from the related nitrophorin proteins, which bind a much larger heme ligand in the central cavity

  17. Structure and ligand-binding properties of the biogenic amine-binding protein from the saliva of a blood-feeding insect vector of Trypanosoma cruzi

    Energy Technology Data Exchange (ETDEWEB)

    Xu, Xueqing; Chang, Bianca W. [NIH/NIAID, 12735 Twinbrook Parkway, Rockville, MD 20852 (United States); Mans, Ben J. [NIH/NIAID, 12735 Twinbrook Parkway, Rockville, MD 20852 (United States); Agricultural Research Council, Onderstepoort 0110 (South Africa); Ribeiro, Jose M. C.; Andersen, John F., E-mail: jandersen@niaid.nih.gov [NIH/NIAID, 12735 Twinbrook Parkway, Rockville, MD 20852 (United States)

    2013-01-01

    Biogenic amine-binding proteins mediate the anti-inflammatory and antihemostatic activities of blood-feeding insect saliva. The structure of the amine-binding protein from R. prolixus reveals the interaction of biogenic amine ligands with the protein. Proteins that bind small-molecule mediators of inflammation and hemostasis are essential for blood-feeding by arthropod vectors of infectious disease. In ticks and triatomine insects, the lipocalin protein family is greatly expanded and members have been shown to bind biogenic amines, eicosanoids and ADP. These compounds are potent mediators of platelet activation, inflammation and vascular tone. In this paper, the structure of the amine-binding protein (ABP) from Rhodnius prolixus, a vector of the trypanosome that causes Chagas disease, is described. ABP binds the biogenic amines serotonin and norepinephrine with high affinity. A complex with tryptamine shows the presence of a binding site for a single ligand molecule in the central cavity of the β-barrel structure. The cavity contains significant additional volume, suggesting that this protein may have evolved from the related nitrophorin proteins, which bind a much larger heme ligand in the central cavity.

  18. Effects of dermatan sulfate derivatives on platelet surface P-selectin expression and protein C activity in blood of inflammatory bowel disease patients

    OpenAIRE

    Ji, Sheng-Li; Du, Hai-Yan; Chi, Yan-Qing; Cui, Hui-Fei; Cao, Ji-Chao; Geng, Mei-yu; Guan, Hua-Shi

    2004-01-01

    AIM: To investigate the effect of dermatan sulfate (DS) derivatives on platelet surface P-selectin expression and blood activated protein C (APC) activity in patients with inflammatory bowel disease (IBD), and to clarity the anti-inflammatory mechanism of DS derivatives.

  19. Comparison of usefulness of C-reactive protein versus white blood cell count to predict outcome after primary percutaneous coronary intervention for ST elevation myocardial infarction

    NARCIS (Netherlands)

    Smit, Jaap Jan J.; Ottervanger, Jan Paul; Slingerland, Robbert J.; Kolkman, J. J. Evelien; Suryapranata, Harry; Hoorntje, Jan C. A.; Dambrink, Jan-Henk E.; Gosselink, A. T. Marcel; de Boer, Menko-Jan; Zijlstra, Felix; van 't Hof, Arnoud W. J.

    2008-01-01

    White blood cell (WBC) count and high-sensitive C-reactive protein (hs-CRP) are both used as markers of inflammation and prognosis after an ST elevation myocardial infarction (STEMI), but it is unknown whether they have independent prognostic value. We investigated the association and independent pr

  20. Renin-angiotensin-aldosterone responsiveness to low sodium and blood pressure reactivity to angiotensin-II are unrelated to cholesteryl ester transfer protein mass in healthy subjects

    NARCIS (Netherlands)

    Krikken, Jan A.; Dallinga-Thie, Geesje M.; Navis, Gerjan; Dullaart, Robin P. F.

    2008-01-01

    Background: The blood pressure increase associated with the cholesteryl ester transfer protein (CETP) inhibitor, torcetrapib is probably attributable to an off-target effect but it is unknown whether activation of the renin-angiotensin-aldosterone system (RAAS) may be related to variation in the pla

  1. Flugmechanik und Flugregelung von Luftschiffen

    OpenAIRE

    Kämpf, Bernhard G.

    2004-01-01

    In der vorliegenden Arbeit wird eine geschlossene algebraische Modellierung der Flugmechanik von Luftschiffen entwickelt und im Rahmen analytischer und numerischer Untersuchungen angewendet. Die Resultate geben eine allgemeine Einsicht in die flugmechanischen Eigenschaften von Luftschiffen und werden in den Entwurf einer Flugreglerstruktur umgesetzt. Neben der Darstellung der Gewichts- und Schubkräfte werden die aerostatischen und thermodynamischen Zusammenhänge dargestellt. Der Schwerpunk...

  2. Identification of an additional class of C3-binding membrane proteins of human peripheral blood leukocytes and cell lines.

    Science.gov (United States)

    Cole, J L; Housley, G A; Dykman, T R; MacDermott, R P; Atkinson, J P

    1985-02-01

    Proteins binding the third component of complement (C3) were isolated by affinity chromatography from surface-labeled solubilized membranes of human peripheral blood cells and cell lines. The isolated molecules were subjected to NaDodSO4/PAGE, and autoradiographs of these gels indicated that C3-binding proteins could be divided into three groups based on Mr: (i) gp200, an approximately 200,000 Mr molecule previously identified as the C3b/C4b receptor or CR1; (ii) gp140, an approximately 140,000 Mr molecule previously identified as the C3d receptor or CR2; and (iii) gp45-70, a heretofore unrecognized group of 45,000-70,000 Mr C3-binding molecules. The cell distribution, Mr, antigenic cross-reactivity, and specificity of gp45-70 were examined. Erythrocytes have no detectable gp45-70, but all leukocyte populations examined possess this group of molecules. On neutrophils and mononuclear phagocytes, CR1 is the predominant C3-binding glycoprotein, but gp45-70 is present on both cell populations and on macrophage and neutrophil cell lines. B plus null cells, chronic lymphocytic leukemia cells, and an Epstein-Barr virus-transformed B-cell line possess CR1, CR2, and gp45-70. On T cells and T-cell lines gp45-70 is the predominant or, in some cases, the only C3-binding protein isolated. gp45-70 is structurally characterized as a broad band or doublet with a mean Mr that is slightly different for each cell population. gp45-70 binds iC3, C3b, and C4b, but not C3d, indicating that the binding region is probably within the C3c portion of C3b. A polyclonal antibody to CR1 and monoclonal antibodies to CR1 and CR2 do not immunoprecipitate gp45-70. While gp45-70 has not been previously characterized on human cells, a C3b-binding glycoprotein of similar Mr is present on rabbit alveolar macrophages. We conclude that gp45-70 is an additional group of membrane proteins present on human leukocytes that possess ligand-binding activity for C3b. PMID:3871945

  3. John von Neumann

    International Nuclear Information System (INIS)

    This article is dedicated to John von Neumann, one of the most outstanding scholars of the 20th century. His life was short but bright, and his contribution to almost all branches of mathematics, as well as to physics, economics, biology, and astronomy was enormous. He constructed some of the first computers and he was among the key persons in the American atomic project. Development of his ideas will continue to play a vital part in various areas of pure and applied mathematics. (from the history of physics)

  4. Acquired von Willebrand Syndrome

    Institute of Scientific and Technical Information of China (English)

    郭涛

    2005-01-01

    @@ Acquired von Willebrand syndrome (AvWS) is kind of bleeding disorder with laboratory findings similar to those in congenital yon Willebrand disease (vWD).AvWS doesn's have any personal or family history of bleeding, but is associated with certain diseases or abnormal conditions or drugs. Although AvWS is being stated as a rare disease, it has gained more and more attention during the past years. Not because of the severity of the disease, but it is more common than we thought and most patients don' t have a proper diagnosis.

  5. Laserbasierte Ortung von Weltraumschrott

    OpenAIRE

    Riede, Wolfgang; Hampf, Daniel; Wagner, Paul; Giesen, Adolf

    2014-01-01

    Raumfahrt ist heute eine der wichtigen Schlüssel- und Kernkompetenzen der modernen Industriegesellschaft. Insbesondere für Streitkräfte oder für ein effektives Katastrophenmanagement sind satellitengestützte Dienste wie z.B. Erdbeobachtung, Kommunikation und Navigation unverzichtbar. Die größte Bedrohung dieser Infrastruktur rührt aus dem Anwachsen der Population von Schrottobjekten. In den niedrigen Erdumlaufbahnen befinden sich derzeit ca. 2/3 der aktiven Satelliten und in mehreren Höhe...

  6. Knockout studies reveal an important role of Plasmodium lipoic acid protein ligase A1 for asexual blood stage parasite survival.

    Directory of Open Access Journals (Sweden)

    Svenja Günther

    Full Text Available Lipoic acid (LA is a dithiol-containing cofactor that is essential for the function of alpha-keto acid dehydrogenase complexes. LA acts as a reversible acyl group acceptor and 'swinging arm' during acyl-coenzyme A formation. The cofactor is post-translationally attached to the acyl-transferase subunits of the multienzyme complexes through the action of octanoyl (lipoyl: N-octanoyl (lipoyl transferase (LipB or lipoic acid protein ligases (LplA. Remarkably, apicomplexan parasites possess LA biosynthesis as well as scavenging pathways and the two pathways are distributed between mitochondrion and a vestigial organelle, the apicoplast. The apicoplast-specific LipB is dispensable for parasite growth due to functional redundancy of the parasite's lipoic acid/octanoic acid ligases/transferases. In this study, we show that LplA1 plays a pivotal role during the development of the erythrocytic stages of the malaria parasite. Gene disruptions in the human malaria parasite P. falciparum consistently were unsuccessful while in the rodent malaria model parasite P. berghei the LplA1 gene locus was targeted by knock-in and knockout constructs. However, the LplA1((- mutant could not be cloned suggesting a critical role of LplA1 for asexual parasite growth in vitro and in vivo. These experimental genetics data suggest that lipoylation during expansion in red blood cells largely occurs through salvage from the host erythrocytes and subsequent ligation of LA to the target proteins of the malaria parasite.

  7. Effects of medium-chain fatty acids and oleic acid on blood lipids, lipoproteins, glucose, insulin, and lipid transfer protein activities

    DEFF Research Database (Denmark)

    Tholstrup, T.; Ehnholm, C.; Jauhiainen, M.;

    2004-01-01

    cholesterol, although this claim is poorly documented. Objective: We compared the effects of a diet rich in either MCFAs or oleic acid on fasting blood lipids, lipoproteins, glucose, insulin, and lipid transfer protein activities in healthy men. Design: In a study with a double-blind, randomized, crossover...... design, 17 healthy young men replaced part of their habitual dietary fat intake with 70 g MCTs (66% 8:0 and 34% 10:0) or high-oleic sunflower oil (89.4% 18:1). Each intervention period lasted 21 d, and the 2 periods were separated by a washout period of 2 wk. Blood samples were taken before and after the...... oleic acid, MCT fat unfavorably affected lipid profiles in healthy young men by increasing plasma LDL cholesterol and triacylglycerol. No changes in the activities of phospholipid transfer protein and cholesterol ester transfer protein were evident....

  8. An ELISA for the quantitation of von Willebrand Factor

    DEFF Research Database (Denmark)

    Vinholt, Pernille Just; Overgaard, Martin; Diederichsen, Axel Cosmus Pyndt;

    2013-01-01

    BACKGROUND: Von Willebrand factor (VWF) is pivotal in arterial thrombosis, and osteoprotegerin (OPG) is besides being a bone protein also related to cardiovascular diseases. OPG can bind VWF, but the significance of this interaction is not known. OBJECTIVES: The aim was to develop an assay for me...

  9. Ozonation of Human Blood Induces a Remarkable Upregulation of Heme Oxygenase-1 and Heat Stress Protein-70

    Directory of Open Access Journals (Sweden)

    Velio Bocci

    2007-01-01

    Full Text Available Heme oxygenase-I (HO-1 has emerged as one of the most protective enzymes and its pleiotropic activities have been demonstrated in a variety of human pathologies. Unpublished observations have shown that HO-1 is induced after the infusion of ozonated blood into the respective donors, and many other experimental observations have demonstrated the efficacy of oxidizing agents. It appeared worthwhile to evaluate whether we could better define the activity of potential inducers such as hydrogen peroxide and ozonated human plasma. Human vascular endothelial cells at confluence were challenged with different concentrations of these inducers and the simultaneous production of nitric oxide (NO; and HO-1 was measured by either measuring nitrite, or bilirubin formation, or/and the immune reactivity of the protein by Western blot using a rabbit antihuman HO-1 and Hsp-70. The results show that production of both NO and HO-1 is fairly dose dependent but is particularly elevated using human plasma after transient exposure to a medium ozone concentration. At this concentration, there is also induction of Hsp-70. The results clarify another positive effect achievable by the use of ozone therapy.

  10. Effects of alcohol-induced human peripheral blood mononuclear cell (PBMC) pretreated whey protein concentrate (WPC) on oxidative damage.

    Science.gov (United States)

    Tseng, Yang-Ming; Chen, Sheng-Yi; Chen, Chien-Hung; Jin, Yi-Ru; Tsai, Shih-Meng; Chen, Ing-Jun; Lee, Jang-Hwa; Chiu, Chzng-Cheng; Tsai, Li-Yu

    2008-09-10

    Excessive alcohol consumption can induce apoptosis in a variety of tissues and influence the antioxidant status in peripheral blood mononuclear cells (PBMC). This paper investigates the effects of whey protein concentrate (WPC) pretreated in PBMC on the apoptosis and antioxidant status after the treatment of alcohol. The results show that the percentages of apoptotic cells in the alcohol-treated group were higher than those in the group without alcohol treatment. Additionally, there was higher glutathione (GSH) peroxidase (GPx) activity when the PBMC were treated with 300 mg/dL of alcohol. With regard to the activity of GSH reductase (GRx), there was higher activity in the group pretreated with WPC than in the group with the treatment of alcohol only. On the contrary, the levels of GSH were reduced after the treatment of alcohol, but there was a higher level of GSH in the group pretreated with WPC. In this study, it was found that the increased level of GSH in PBMC might not be attributed to the effect of GRx because there was still a higher level of GSH in the group with the treatment of WPC and BCNU (a GRx inhibitor) in this study. The results indicated that PBMC pretreated with WPC might ameliorate alcohol-induced effects such as imbalance of the antioxidant status. PMID:18700777

  11. The Changes of Protein Kinase C Activity in Peripheral Blood Lymphocytes in the Patients with Obstructive Jaundice and the Implication

    Institute of Scientific and Technical Information of China (English)

    2001-01-01

    The roles of protein kinase C (PKC) signal pathway in the pathogenesis of obstructive jaundice were studied. PKC from cytosolic and membrane fractions of peripheral blood lymphocytes (PBL) in 51 patients with obstructive jaundice and 16 cases of normal controls was isolated and purified. The activities of PKC were determined by radioactive isotope γ-32P-ATP-catalyzing assay. The results showed that the total PKC activities in PBL in the patients with obstructive jaundice were significantly increased as compared with those in the normal controls (P<0.01). Moreover, the membrane PKC activities and their percentages of the total PKC activities were higher in obstructive jaundice group than in those in the normal controls (P<0.05). The total PKC activities in PBL in the patients with obstructive jaundice were significantly positively correlated with the levels of soluble IL-2 receptor (sIL-2R) (r=0.58, P<0.01) and the degree of jaundice (T-BIL) (r=0.67, P<0.01) in serum. It was concluded that the activities of PKC signal pathway was related with the degree of T-BIL. PKC signal pathway might took part in the activation of T-lymphocytes in the patients with obstructive jaundice and play an important role in the immune regulation and the assessment of pathosis in the patients with obstructive jaundice.

  12. Verhalten von passiv betriebenen Sauggreifern unter der Krafteinwirkung von Kletterrobotern

    OpenAIRE

    Simons, Florian

    2006-01-01

    Für den wirtschaftlichen Einsatz von Kletterrobotern auf Glasfassaden zur Durchführung von Wartungs- und Inspektionsaufgaben ist die Einsetzbarkeit auf einer möglichst großen Anzahl an Gebäuden notwendig. Der Roboter muss sich dafür möglichst ohne externe Energieversorgung und unabhängig von gebäudeseitigen Installationen wie Dachkränen und speziellen notwendigen Kletterstrukturen über Vakuumelemente direkt auf den Glaselementen halten und bewegen können. Dies stellt hohe Anforderungen an Gew...

  13. Preferential binding of 4-hydroxynonenal to lysine residues in specific parasite proteins in plakortin-treated Plasmodium falciparum-parasitized red blood cells

    Directory of Open Access Journals (Sweden)

    Evelin Schwarzer

    2015-12-01

    Full Text Available The data show the frequencies by which the amino acid residues lysine, histidine and cysteine of six proteins of the malaria parasite Plasmodium falciparum are post-translationally modified by the lipoperoxydation endproduct 4-hydroxynonenal after challenging the parasitized red blood cell with plakortin. Plakortin is an antimalarial endoperoxide whose molecular anti-parasitic effect is described in Skorokhod et al. (2015 [1]. Plakortin did not elicit hemoglobin leakage from host red blood cells and did not oxidize reduced glutathione.

  14. The Effects of Different Levels of Dietary Protein and L-Carnitine on Blood Sugar and Lipids of the New GIFT Strain of Juvenile Nile Tilapia (Oreochromis niloticus)

    OpenAIRE

    Gang Chen; Minghui Zhang; Jiandong Zhang; Hongbiao Dong; Hui Zhou; Baogui Tang; Jiansheng Huang; Gang Shi; Ling Jiang; Zhaohe Wu

    2009-01-01

    The new GIFT (Genetically Improved Farmed Tilapia) strain of Nile tilapia is a popular cultivated fish in Asia, but intensive aquaculture using nutritionally imbalanced feed has led to disorder of lipid metabolisms. An 8-week feeding experiment was conducted in order to assess the effects of different levels of L-carnitine (0, 200, 400, 600, and 800 mg/kg) and dietary protein (22, 25, and 28%) on blood sugar and blood lipid contents of the new juvenile GIFT strain of Nile tilapia. Results sho...

  15. Synthese und Eigenschaften von Kohlenstoffnitriden

    OpenAIRE

    Horvath-Bordon, Elisabeta

    2004-01-01

    In der hier vorliegenden Arbeit über die „Synthese und Eigenschaften von Kohlenstoffnitriden“ wird im ersten Teil über die Synthese, die Strukturaufklärung, thermische Stabilität und die optischen Eigenschaften von Tri-s-triazin Derivaten (Cyamelurate, Melonate, Melem usw.) berichtet. Weil sich molekulare und polymere Tri-s-triazin Derivaten relativ einfach in reiner Form darstellen lassen, wurden sie als Precursoren zur Herstellung von harten Kohlenstoffnitriden ausgewählt. Im zweiten Teil w...

  16. Kriterien zur Beurteilung von Fahrerassistenzsystemen

    OpenAIRE

    Weller, Gert; Schlag, Bernhard

    2002-01-01

    Der Markt für Fahrerassistenzsysteme wächst nach Presseinformationen von Herstellern um jährlich 15%. Die OECD (2003) geht von einem erheblichen Potential von FAS zur Reduzierung der Unfallzahlen oder zumindest der Unfallschwere aus. Ein weiterer Nutzen liegt in der angestrebten Erhöhung des Komforts und einer Optimierung der Beanspruchung des Fahrers. Andererseits können gerade diese an sich positiven Auswirkungen durch die Reaktion des Menschen auf Veränderungen im System Fahrer-Fa...

  17. Characterization of a novel mutation in the von Willebrand factor propeptide in a distinct subtype of recessive von Willebrand disease

    DEFF Research Database (Denmark)

    Lanke, Elsa; Kristoffersson, Ann-Charlotte; Philips, Malou; Holmberg, Lars; Lethagen, Stefan

    2008-01-01

    von Willebrand factor (VWF) is a plasma protein that consists of a series of multimers of which the high-molecular-weight VWF multimers are the most potent in platelet adhesion and aggregation. The propeptide of the VWF (VWFpp) is known to be essential in the process of multimer assembly. Genetic...... studies were performed in a patient with a phenotype of von Willebrand disease (VWD) characterized by very low plasma factor VIII and VWF levels and a VWF consisting of only a dimeric band and total absence of all multimers in plasma. The patient was found to be homozygous for the novel C570S mutation...

  18. Vernetzungspotential durch Nutzung von Normdaten (am Beispiel von GND / BEACON)

    OpenAIRE

    Lordick, Harald

    2015-01-01

    Der Workshop-Beitrag erläutert an praktischen Beispielen, wie Online-Publikationen von ihrer Anreicherung mit IDs der Gemeinsamen Normdatei (GND) profitieren: durch bessere Sichtbarkeit und Recherchierbarkeit, durch Vernetzung mit weiteren Datenangeboten sowie durch Auflösung von Ambiguität. Skizziert wird der Einstieg in das Thema "Linked Data / Semantic Web" als niedrigschwelliger Workflow: Daten mit Ids der GND anreichern, Web-Schnittstelle einrichten, BEACON-D...

  19. Clearance of von Willebrand factor. : Clearance of von Willebrand factor

    OpenAIRE

    Denis, Cécile; Christophe, Olivier; Oortwijn, Beatrijs,; Lenting, Peter,

    2008-01-01

    The life cycle of von Willebrand factor (VWF) comprises a number of distinct steps, ranging from the controlled expression of the VWF gene in endothelial cells and megakaryocytes to the removal of VWF from the circulation. The various aspects of VWF clearance have been the objects of intense research in the last few years, stimulated by observations that VWF clearance is a relatively common component of the pathogenesis of type 1 von Willebrand disease (VWD). Moreover, improving the survival ...

  20. Insulin Receptor Antibody-α-N-Acetylglucosaminidase Fusion Protein Penetrates the Primate Blood-Brain Barrier and Reduces Glycosoaminoglycans in Sanfilippo Type B Fibroblasts.

    Science.gov (United States)

    Boado, Ruben J; Lu, Jeff Zhiqiang; Hui, Eric Ka-Wai; Lin, Huilan; Pardridge, William M

    2016-04-01

    Mucopolysaccharidosis Type IIIB (MPSIIIB) is caused by mutations in the gene encoding the lysosomal enzyme, α-N-acetylglucosaminidase (NAGLU). MPSIIIB presents with severe disease of the central nervous system, but intravenous NAGLU enzyme replacement therapy has not been developed because the NAGLU enzyme does not cross the blood-brain barrier (BBB). A BBB-penetrating form of the enzyme was produced by re-engineering NAGLU as an IgG-enzyme fusion protein, where the IgG domain is a monoclonal antibody (mAb) against the human insulin receptor (HIR). The HIRMAb traverses the BBB via transport on the endogenous insulin receptor and acts as a molecular Trojan horse to ferry the fused NAGLU across the BBB from blood. The NAGLU was fused to the carboxyl terminus of each heavy chain of the HIRMAb via an extended 31-amino acid linker, and the fusion protein is designated HIRMAb-LL-NAGLU. The fusion protein retains high affinity binding to the HIR, and on a molar basis has an enzyme activity equal to that of recombinant human NAGLU. Treatment of MPSIIIB fibroblasts with the fusion protein normalizes intracellular NAGLU enzyme activity and reduces sulfate incorporation into intracellular glycosoaminoglycan. The fusion protein is targeted to the lysosomal compartment of the cells as shown by confocal microscopy. The fusion protein was radiolabeled with the [(125)I]-Bolton-Hunter reagent and injected intravenously in the adult Rhesus monkey. The fusion protein was rapidly cleared from plasma by all major peripheral organs. The high brain uptake of the fusion protein, 1% injected dose/brain, enables normalization of brain NAGLU enzyme activity with a therapeutic dose of 1 mg/kg. The HIRMAb-LL-NAGLU fusion protein is a new treatment of the brain in MPSIIIB, which can be administered by noninvasive intravenous infusion. PMID:26910785

  1. Dr. Wernher Von Braun

    Science.gov (United States)

    1960-01-01

    On September 8, 1960 President Dwight D. Eisenhower visited Huntsville, Alabama to dedicate a new NASA field center in honor of General George C. Marshall, Eisenhower's wartime colleague and the founder of the famous Marshall Plan for European recover after World War II. The new George C. Marshall Space Flight Center was placed under the control of Dr. Wernher Von Braun shown here talking with President Eisenhower. As parto f his remarks dedicating the center, President Eisenhowe refereed to General Marshall as a 'man of yar, yet a builder of peace'. the Marshall Center's first major assignment including building the huge Saturn V rocket that launched human beings on their first journey to the surface of the moon in 1969.

  2. Pathogenesis, clinical picture and treatment of von Willebrand's disease.

    Science.gov (United States)

    Lethagen, S R

    1995-12-01

    Von Willebrand's disease is probably the most common congenital bleeding disorder, with a prevalence close to 1% in some epidemiological studies. The disease is caused by a quantitative deficiency or a qualitative defect of the von Willebrand factor, which is a multimeric glycoprotein consisting of subunits of 2050 amino acids. The size of multimers ranges from approximately 500 kDa to 20 MDa. Each subunit consist of repeated domain structures. Several functional domains have been identified which can bind such structures as platelet receptors glycoprotein Ib or IIb/IIIA, heparin, collagen or factor VIII. The von Willebrand factor has two main functions in haemostasis, to promote normal platelet adhesion and to be a carrier protein for factor VIII. Von Willebrand's disease is divided into three major types and several subtypes depending on the quantity and quality of the von Willebrand factor in plasma and platelets. A new classification has recently been proposed. Typical symptoms are mucosal bleeding, easy bruising and increased bleeding tendency in connection with tooth extractions and other invasive procedures. Severe cases may have joint bleeding and other haemophilia-like bleeding. Desmopressin is the treatment of choice in mild cases, whereas more severe cases need treatment with factor VIII concentrates. PMID:8652143

  3. Blood Clots

    Science.gov (United States)

    ... Index A-Z Blood Clots Blood clots are semi-solid masses of blood that can be stationary (thrombosis) ... treated? What are blood clots? Blood clots are semi-solid masses of blood. Normally, blood flows freely through ...

  4. Relationship between vitamin D-binding protein polymorphisms and blood vitamin D level in Korean patients with COPD

    Directory of Open Access Journals (Sweden)

    Park YM

    2016-04-01

    Full Text Available Youngmok Park,1 Young Sam Kim,1 Young Ae Kang,1 Ju Hye Shin,1 Yeon Mok Oh,2 Joon Beom Seo,3 Ji Ye Jung,1 Sang Do Lee2 On behalf of the KOLD study 1Division of Pulmonology, Department of Internal Medicine, Severance Hospital, Institute of Chest Diseases, Yonsei University College of Medicine, 2Department of Pulmonary and Critical Care Medicine, Clinical Research Center for Chronic Obstructive Airway Diseases, 3Department of Radiology and Research Institute of Radiology, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Republic of Korea Background: In chronic obstructive pulmonary disease (COPD, the blood vitamin D3 level is generally low, and genetic polymorphisms of vitamin D-binding protein encoded by the GC gene are associated with COPD development. In this study, we examined the relationship between GC polymorphisms and plasma vitamin D3 level in Korean patients with COPD. Methods: The study included 175 COPD patients from the Korean Obstructive Lung Disease Cohort. Multivariate analysis was conducted with adjustment for age, body mass index (BMI, lung function, smoking status, smoking amount, and seasonal variation in blood vitamin D level. Vitamin D deficiency was defined as a plasma 25-hydroxyvitamin D3 level lower than 20 ng/mL. Results: The mean plasma vitamin D3 level was 17.5 ng/mL. The GC1F variant (44.3% and genotype 1F-2 (27.4% were the most common. The plasma vitamin D3 level was lower in patients with the GC2 variant (estimated =-3.73 ng/mL and higher in those with genotype 1F-1S (estimated =4.08 ng/mL. The GC2 variant was a significant risk factor for vitamin D deficiency (odds ratio =2.41. Among COPD clinical parameters, vitamin D deficiency was associated with a lower ratio of forced expiratory volume in 1 second to forced vital capacity (FEV1/FVC regardless of GC polymorphisms. FEV1/FVC was higher in patients with genotype 1F-1F (estimated =3.61% and lower in those with genotype 1F-2 (estimated =-3.31%. The

  5. What Should You Know about Blood Disorders in Women?

    Science.gov (United States)

    ... Although there are no cures for bleeding disorders, treatment is available to control symptoms and help women avoid complications and invasive procedures. Related Links Deep Vein Thrombosis Hemophilia Von Willebrand Disease Blood Disorders Home A- ...

  6. Correlation of serum C-reactive protein, white blood count and neutrophil percentage with histopathology findings in acute appendicitis

    Directory of Open Access Journals (Sweden)

    Xharra Shefki

    2012-08-01

    Full Text Available Abstract Background Acute appendicitis is one of the most common surgical emergencies. Accurate diagnosis of acute appendicitis is based on careful history, physical examination, laboratory and imaging investigation. The aim of the study is to analyze the role of C-reactive protein (CRP, white blood count (WBC and Neutrophil percentage (NP in improving the accuracy of diagnosis of acute appendicitis and to compare it with the intraoperative assessment and histopathology findings. Materials and methods This investigation was a prospective double blinded clinical study. The study was done on 173 patients surgically treated for acute appendicitis. The WBC, NP, and measurement of CRP were randomly collected pre-operatively from all involved patients. Macroscopic assessment was made from the operation. Appendectomy and a histopathology examination were performed on all patients. Gross description was compared with histopathology results and then correlated with CRP, WBC, and NP. Results The observational accuracy was 87,3%, as compared to histopathological accuracy which was 85.5% with a total of 173 patients that were operated on. The histopathology showed 25 (14.5% patients had normal appendices, and 148 (85.5% patients had acutely inflamed, gangrenous, or perforated appendicitis. 52% were male and 48% were female, with the age ranging from 5 to 59 with a median of 19.7. The gangrenous type was the most frequent (52.6%. The WBC was altered in 77.5% of the cases, NP in 72.3%, and C-reactive protein in 76.9% cases. In those with positive appendicitis, the CRP and WBC values were elevated in 126 patients (72.8%, whereas NP was higher than 75% in 117 patients (67.6%. Out of 106 patients with triple positive tests, 101 (95.2% had appendicitis. The sensitivity, specificity, and positive predictive values of the 3 tests in combination were 95.3%, 72.2%, and 95.3%, respectively. Conclusion The raised value of the CRP was directly related to the severity of

  7. Chronic effects of maternal ethanol and low-protein intake on growth and blood measurements of beagle pups

    International Nuclear Information System (INIS)

    Pups used in this study were born to nulliparous, purebred female beagles fed either 17% control (CP) or 8.5% low protein (LP) diets and were given twice daily either 1.8 g/kg ethanol (E) or an equivalent isocaloric dose of sucrose (S) throughout pregnancy. After parturition, all mothers were fed the CP diet and no E or S. On day 1 and each week up to 4 weeks, the weight (WT), crown-rump length (LT) and head circumference (HC) of the pups were measured. These measurements were taken for a post-weaning subset at 6, 8 and 10 weeks. Blood samples were collected each week. At birth, mean WT, LT and HC were significantly lower in pups from E-mothers as compared to S-mothers with either CP or LP diets. The birth WT, LT and HC were significantly lower when mothers were fed LP as compared to the CP diet with either S or E. The prenatal effects of E and LP were significantly associated with lower pup WT, HT and hematocrit values, but not HC up to 4 weeks. At 10 weeks, the growth measurements and hematocrits were significantly lower with prenatal E exposure but not with LP. Pup red cell levels of folate were significantly lower with prenatal E during the first 4 weeks, whereas the effect of prenatal LP but not E was significant at 10 weeks. These data suggest that growth parameters and hematocrit values of pups prenatally exposed to E do not catch up to those of pups from S-mothers fed either diet

  8. IPP-rich milk protein hydrolysate lowers blood pressure in subjects with stage 1 hypertension, a randomized controlled trial

    Directory of Open Access Journals (Sweden)

    Kloek Joris

    2010-11-01

    Full Text Available Abstract Background Milk derived peptides have been identified as potential antihypertensive agents. The primary objective was to investigate the effectiveness of IPP-rich milk protein hydrolysates (MPH on reducing blood pressure (BP as well as to investigate safety parameters and tolerability. The secondary objective was to confirm or falsify ACE inhibition as the mechanism underlying BP reductions by measuring plasma renin activity and angiotensin I and II. Methods We conducted a randomized, placebo-controlled, double blind, crossover study including 70 Caucasian subjects with prehypertension or stage 1 hypertension. Study treatments consisted of daily consumption of two capsules MPH1 (each containing 7.5 mg Isoleucine-Proline-Proline; IPP, MPH2 (each containing 6.6 mg Methionine-Alanine-Proline, 2.3 mg Leucine-Proline-Proline, 1.8 mg IPP, or placebo (containing cellulose for 4 weeks. Results In subjects with stage 1 hypertension, MPH1 lowered systolic BP by 3.8 mm Hg (P = 0.0080 and diastolic BP by 2.3 mm Hg (P = 0.0065 compared with placebo. In prehypertensive subjects, the differences in BP between MPH1 and placebo were not significant. MPH2 did not change BP significantly compared with placebo in stage I hypertensive or prehypertensive subjects. Intake of MPHs was well tolerated and safe. No treatment differences in hematology, clinical laboratory parameters or adverse effects were observed. No significant differences between MPHs and placebo were found in plasma renin activity, or angiotensin I and II. Conclusions MPH1, containing IPP and no minerals, exerts clinically relevant BP lowering effects in subjects with stage 1 hypertension. It may be included in lifestyle changes aiming to prevent or reduce high BP. Trial registration ClinicalTrials.gov NCT00471263

  9. Die Kosmogonie Anton von Zachs.

    Science.gov (United States)

    Brosche, P.

    In his "Cosmogenische Betrachtungen" (1804), Anton von Zach rediscovered - probably independently - some aspects of the theories of Kant and Laplace. More originally, he envisaged also the consequences of an era of heavy impacts in the early history of the Earth.

  10. Maternal and Cord Blood Levels of Serum Amyloid A, C-Reactive Protein, Tumor Necrosis Factor-α, Interleukin -1β, and Interleukin-8 During and After Delivery

    Directory of Open Access Journals (Sweden)

    Luciane Marzzullo Cicarelli

    2005-01-01

    after delivery and try to correlate these proteins with tumor necrosis factor-α, interleukin -1β, and interleukin-8. Acute-phase proteins and cytokines were measured by ELISA in 24 healthy pregnant women undergoing vaginal delivery or Cesarean section. Cord blood samples in addition to maternal blood were collected. SAA and CRP reached the maximum maternal serum levels 24 hours after delivery, while cytokines remained constant over time. SAA and CRP were significantly higher in maternal serum than in newborn's (P<.001 at the moment of delivery. SAA and CRP, regardless of the type of delivery, reproduce the common pattern observed in most inflammatory conditions. Proinflammatory cytokine serum levels do not mirror the increase in SAA and CRP levels.

  11. Selektives Lasersintern von teilkristallinen Thermoplasten

    OpenAIRE

    Rietzel, Dominik; Kühnlein, Florian; Drummer, Dietmar

    2010-01-01

    Da eine flexible Fertigung im Bereich von Losgrößen zwischen 1-1000 Stück in vielen Wirtschaftszweigen vermehrt an Bedeutung gewinnt, steigt das Interesse an Verfahren wie dem Selektiven Lasersintern. Dennoch sollen die Eigenschaften von in Serie eingesetzten Werkstoffen erreicht werden. Aufgrund der bestehenden werkstofflichen Restriktionen auf Polyamid 12 wird aktuell an der Verarbeitung anderer teilkristalliner Thermoplaste geforscht. In diesem Beitrag werden die sich in der Markteinführun...

  12. Planung von CLIL-Unterricht

    OpenAIRE

    Leisen, Josef

    2015-01-01

    Der Beitrag formuliert die Sprachlernbedingungen und die Leitlinien des Sprachlernens im Fach und nennt Merkmale des guten integrierten Fach- und Sprachlehrens. Ausgehend von den Sprachproblemen, die sich im CLIL-Unterricht auftun, wird der sprachsensible CLIL-Unterricht definiert, und es werden Anregungen zur Gestaltung gegeben. Sprachliche Standardsituationen umfassen die kommunikativen Situationen im CLIL-Unterricht, die beim fachlichen Lernen auftreten und von der CLIL-Lehrkraft professio...

  13. Von der Konzeption zur Praxis

    OpenAIRE

    Emer, Wolfgang (Akad. Oberrat)

    2015-01-01

    Die kumulative Dissertation zur Projektdidaktik trägt den Titel „Von der Konzeption zur Praxis: Zur Entwicklung der Projektdidaktik am Oberstufen-Kolleg Bielefeld und ihre Impulsgebung und Modellbildung für das deutsche Regelschulwesen“. Die Dissertation versteht sich als beispielgebende Umsetzung und Implementierung der Projektdidaktik für das Regelschulsystem. Auf der Basis von 22 bereits erschienenen Publikationen und einer Monographie werden mit fünf methodischen Zugriffen (bildungshistor...

  14. Investitionsentscheidungen von Familien- und Nichtfamilienunternehmern

    OpenAIRE

    Zellweger, Thomas

    2006-01-01

    Die wissenschaftliche Literatur zu Investitionsentscheidungen geht auf die Arbeiten von Fisher zurück, insbesondere sein Buch Theory of Interest (Fisher, 1930). Eine der zentralen Erkenntnisse wurde als "Fisher Separation" bekannt, welche eine Rechtfertigung für die "Netto-Barwert-Regel" einerseits und die Trennung von Eigentum und Management andererseits liefert. Das Separationstheorem besagt, dass in einer deterministischen Welt mit perfekten Kapitalmärkten Entscheidungen über Produktio...

  15. Kinetics of Dengue Non-Structural Protein 1 Antigen and IgM and IgA Antibodies in Capillary Blood Samples from Confirmed Dengue Patients

    OpenAIRE

    Matheus, Séverine; Pham, Thai Binh; Labeau, Bhetty; Huong, Vu Thi Que; Lacoste, Vincent; Deparis, Xavier; Marechal, Vincent

    2014-01-01

    Large-scale epidemiological surveillance of dengue in the field and dengue patient management require simple methods for sample collection, storage, and transportation as well as effective diagnostic tools. We evaluated the kinetics of three biological markers of dengue infection—non-structural protein 1 (NS1) antigen, immunoglobulin M (IgM), and IgA—in sequential capillary blood samples collected from fingertips of confirmed dengue patients. The overall sensitivities and specificities of the...

  16. Combining Viral Vectored and Protein-in-adjuvant Vaccines Against the Blood-stage Malaria Antigen AMA1: Report on a Phase 1a Clinical Trial

    OpenAIRE

    Susanne H. Hodgson; Choudhary, Prateek; Elias, Sean C; Milne, Kathryn H; Thomas W Rampling; Biswas, Sumi; Ian D Poulton; Miura, Kazutoyo; Douglas, Alexander D.; Alanine, Daniel GW; Illingworth, Joseph J.; de Cassan, Simone C.; ZHU, DAMING; Nicosia, Alfredo; Long, Carole A.

    2014-01-01

    The development of effective vaccines against difficult disease targets will require the identification of new subunit vaccination strategies that can induce and maintain effective immune responses in humans. Here we report on a phase 1a clinical trial using the AMA1 antigen from the blood-stage Plasmodium falciparum malaria parasite delivered either as recombinant protein formulated with Alhydrogel adjuvant with and without CPG 7909, or using recombinant vectored vaccines—chimpanzee adenovir...

  17. Coronary vasomotor and blood flow responses to isoflavone-intact soy protein in subjects with coronary heart disease or risk factors for coronary heart disease

    OpenAIRE

    Webb, Carolyn M.; Hayward, Christopher S.; Mason, Mark J.; Ilsley, Charles D.; Collins, Peter

    2008-01-01

    Abstract Animal data suggest favourable coronary vasomotor actions of isoflavones however the effects of isoflavones on the human coronary circulation are undetermined. We therefore investigated the effects of short-term isoflavone-intact soy protein ingestion on basal coronary arterial tone and stimulated vasoreactivity and blood flow in patients with coronary heart disease (CHD) or risk factors for CHD. Seventy-one subjects were randomised, double-blind, to isoflavone-intact so...

  18. Mycobacterium avium subsp. paratuberculosis Infection Causes Suppression of RANTES, Monocyte Chemoattractant Protein 1, and Tumor Necrosis Factor Alpha Expression in Peripheral Blood of Experimentally Infected Cattle

    OpenAIRE

    Buza, Joram J.; Mori, Yasuyuki; Bari, Abusaleh M.; Hikono Aodon-geril, Hirokazu; Hirayama, Sachiyo; Shu, Yujing; Momotani, Eiichi

    2003-01-01

    Blood from cattle with subclinical Mycobacterium avium subsp. paratuberculosis infection was stimulated with M. avium subsp. paratuberculosis antigens, and expression of interleukin-1β (IL-1β), tumor necrosis factor alpha (TNF-α), RANTES, monocyte chemoattractant protein 1 (MCP-1), and IL-8 was measured. Expression of TNF-α, RANTES, and MCP-1 was lower in infected than in uninfected cattle. The reduced response may weaken protective immunity and perpetuate infection.

  19. STUDY OF ERYTHROCYTE SEDIMENTATION RATE,SERUM TOTAL PROTEIN, SERUM ALBUMIN,SERUM GLOBULIN AND RED BLOOD CELL COUNT IN DIFFERENT PHASES OF MENSTRUAL CYCLE

    OpenAIRE

    Shilpa,; Girish M; Patil, MR; Malipatil

    2014-01-01

    : BACKGROUND: Menstruation is a phenomenon unique to females and nearly universal experience in women's lives and is poorly understood. It is characterized by co-ordinate sequence of hormonal changes but the changes that occur in the distribution of hematological parameters, biochemical parameters have not been clearly established. AIMS: To compare Erythrocyte Sedimentation Rate (ESR), Serum total protein, Serum albumin, Serum globulin and Red Blood Cell count (RBC) in dif...

  20. Assessment of the effect of phytic acid on the labeling of blood cells and plasma proteins with Technetium-99m

    International Nuclear Information System (INIS)

    Blood elements labeled with technetium-99m (99m Tc) have been used in various procedures in nuclear medicine. We have investigated if phytic acid (PHY) could alter the labeling of blood elements with 99m Tc. Blood was incubated with different concentrations of PHY. Stannous chloride and 99mTc, as sodium pertechnetate, were added. Blood was centrifuged and plasma (P) and blood cell (BC) were isolated. Samples of P and BC were also precipitated with trichloroacetic acid and centrifuged, and insoluble (IF) and soluble (SF) fractions were separated. The percentages of radioactivity (%ATI) in BC, IF-P and IF-BC were calculated. The %ATI decreased significantly (p 99m Tc with possible undesirable effects, it is relevant to verify the necessity to repeat the examination and to evaluate the increase of the radiation dose to the patient. (author)

  1. Effect of dietary energy and protein on the performance, egg quality, bone mineral density, blood properties and yolk fatty acid composition of organic laying hens

    Directory of Open Access Journals (Sweden)

    Md. Rakibul Hassan

    2013-02-01

    Full Text Available An experiment was conducted to evaluate the effect of dietary metabolizable energy (ME and crude protein (CP on the performance, egg quality, blood properties, bone characteristics and yolk fatty acid composition of organic laying hens. At 23 weeks, a total of 600 Brown nick laying hens were randomly distributed into 24 outdoor pens (4 replicate pens/treatment; 25 birds/pen and were given (2750, 2775 and 2800 kcal of ME/kg and CP (16 and 17% resulting in a 3×2 factorial arrangement of organic dietary treatments. The experiment lasted 23 weeks. The performance of laying hens were not affected by the dietary treatment while the egg weight was increased with energy and CP levels in the diet (P<0.05. Serum total protein was not affected by dietary energy and protein level. Total cholesterol and triglyceride tend to reduce with the increasing amount of CP in the diet. Thereafter, bone and egg quality characteristics were numerically increased in dietary 2775 kcal of ME/kg and 16% CP treatment. On the other hand, docosahexanoic acid content in egg yolk was higher (P<0.01 in 2750 kcal of ME/kg and 17% CP treatment. As a result, the performance, blood and fatty acid composition were maximized in 2750 kcal of ME/kg and 16% CP treatment. Thus, dietary 2750-2775 kcal of ME/kg and 16% CP may enhance performance, blood and fatty acid composition of organic laying hens.

  2. Detection of transgenic and endogenous plant DNA fragments and proteins in the digesta, blood, tissues, and eggs of laying hens fed with phytase transgenic corn.

    Directory of Open Access Journals (Sweden)

    Qiugang Ma

    Full Text Available The trials were conducted to assess the effects of long-term feeding with phytase transgenic corn (PTC to hens on laying performance and egg quality, and investigate the fate of transgenic DNA and protein in digesta, blood, tissues, and eggs. Fifty-week old laying hens (n = 144 were fed with a diet containing 62.4% PTC or non-transgenic isogenic control corn (CC for 16 weeks. We observed that feeding PTC to laying hens had no adverse effect on laying performance or egg quality (P>0.05 except on yolk color (P<0.05. Transgenic phyA2 gene and protein were rapidly degraded in the digestive tract and were not detected in blood, heart, liver, spleen, kidney, breast muscle, and eggs of laying hens fed with diet containing PTC. It was concluded that performance of hens fed diets containing PTC, as measured by egg production and egg quality, was similar to that of hens fed diets formulated with CC. There was no evidence of phyA2 gene or protein translocation to the blood, tissues, and eggs of laying hens.

  3. Characterization of aberrant splicing of von Willebrand factor in von Willebrand disease: an underrecognized mechanism.

    Science.gov (United States)

    Hawke, Lindsey; Bowman, Mackenzie L; Poon, Man-Chiu; Scully, Mary-Frances; Rivard, Georges-Etienne; James, Paula D

    2016-07-28

    Approximately 10% of von Willebrand factor (VWF) gene mutations are thought to alter messenger RNA (mRNA) splicing through disruption of consensus splice sites. This mechanism is likely underrecognized and affected by mutations outside consensus splice sites. During VWF synthesis, splicing abnormalities lead to qualitative defects or quantitative deficiencies in VWF. This study investigated the pathologic mechanism acting in 3 von Willebrand disease (VWD) families with putative splicing mutations using patient-derived blood outgrowth endothelial cells (BOECs) and a heterologous human embryonic kidney (HEK 293(T)) cell model. The exonic mutation c.3538G>A causes 3 in-frame splicing variants (23del, 26del, and 23/26del) which cannot bind platelets, blood coagulation factor VIII, or collagen, causing VWD through dominant-negative intracellular retention of coexpressed wild-type (WT) VWF, and increased trafficking to lysosomes. Individuals heterozygous for the c.5842+1G>C mutation produce exon 33 skipping, exons 33-34 skipping, and WT VWF transcripts. Pathogenic intracellular retention of VWF lacking exons 33-34 causes their VWD. The branch site mutation c.6599-20A>T causes type 1 VWD through mRNA degradation of exon 38 skipping transcripts. Splicing ratios of aberrant transcripts and coexpressed WT were altered in the BOECs with exposure to shear stress. This study provides evidence of mutations outside consensus splice sites disrupting splicing and introduces the concept that VWF splicing is affected by shear stress on endothelial cells. PMID:27317792

  4. Poly(ε-caprolactone) modified with fusion protein containing self-assembled hydrophobin and functional peptide for selective capture of human blood outgrowth endothelial cells.

    Science.gov (United States)

    Huang, Yujian; Zhang, Suai; Niu, Baolong; Wang, Dandan; Wang, Zefang; Feng, Shuren; Xu, Haijin; Kong, Deling; Qiao, Mingqiang

    2013-01-01

    Human blood outgrowth endothelial cells (HBOECs)-specific binding peptide, TPSLEQRTVYAK (TPS), was proposed to be applied on autologous cell therapy for treating cardiovascular diseases. Hydrophobins, as a family of self-assembly proteins originated from fungi, have demonstrated unique characteristics to modulate surface properties of other materials coated with these amphiphilic proteins in previous studies. In this report, a fusion protein which was composed of class I hydrophobin HGFI originated from Grifola frondosa and functional peptide TPS was expressed by Pichia pastoris expression system. Then, we purified this fusion protein by ultrafiltration and reverse-phase high performance liquid chromatography. Water contact angle, X-ray photoelectron spectroscopy measurements indicated that the surface properties of hydrophobin were greatly preserved by this fusion protein while comparing with wild HGFI. Cell binding assay showed that this fusion protein demonstrated specific binding property to HBOECs while coating on biodegradable poly(ε-caprolactone) (PCL) grafts in the presence of fetal bovine serum, whereas HGFI-coated PCL non-selectively enhanced all types of cells attachments. Methylthiazol tetrazolium assay was employed to verify the cytocompatibility of this fusion protein-based material. This work presented a new perspective to apply hydrophobin in tissue engineering and regenerative medicine and provided an alternative approach to study endothelial progenitor cells. PMID:23010042

  5. The Effect on Cognition of Mitochondrial Respiratory System Proteins in Peripheral Blood Mononuclear Cells in the Course of Lung Cancer.

    Science.gov (United States)

    Michalak, S; Rybacka-Mossakowska, J; Gazdulska, J; Gołda-Gocka, I; Ramlau, R

    2016-01-01

    Peripheral blood mononuclear cells (PBMC) represent an easily available population of cells for the studies on remote effects of lung cancer. NADH dehydrogenase (ubiquinone) Fe-S protein-1 (Ndufs1), a marker of mitochondrial complex I, and mitochondrially encoded cytochrome c oxidase 1 (MTCO1), a marker of complex IV, may participate in cognitive decline during the course of lung cancer. In this study, Ndufs1 and MTCO1 expression in PBMC was evaluated by means of ELISA in 80 lung cancer patients. Mini-Mental State Examination (MMSE) were conducted Trail Making Tests (TMT-A and TMT-B) at baseline and after the 6 months' follow-up. Autoantibodies were identified by means of indirect immunofluorescence and line blot. We found that enhanced levels of Ndufs1 in PBMC were related to impaired cognitive performance; TMT-A of 13.6 ± 3.1 s and TMT-B of 162.5 ± 46.4 s compared with 8.6 ± 4.5 s (p = 0.003) and 124.8 ± 51.8 s (p TMT-A - τb = 0.30; p = 0.001), and TMT-B - τb = 0.199; p = 0.012) after the 6 months' follow-up. Higher MTCO1 expression was accompanied by worse TMT-A results than in case of inhibited MTCO1; 11.1 ± 5.8 s vs. 8.5 ± 4.1 s; respectively; p = 0.048. MTCO1 expression was correlated with TMT-A results (τb = 0.17; p = 0.034) at baseline. We conclude that stimulation of PBMC mitochondrial function in lung cancer patients is associated with cognitive impairment. Mitochondrial dysfunction in PBMC may reflect cytotoxicity responsible for neurological deficits. PMID:26987334

  6. Changes in blood levels of proteinase inhibitors, pregnancy zone protein, steroid carriers and complement factors induced by oral contraceptives

    DEFF Research Database (Denmark)

    Nielsen, C H; Poulsen, H K; Teisner, B;

    1993-01-01

    Three low-dose oral contraceptives Trinordiol, Gynatrol, and Marvelon, containing ethinylestradiol (EE) in combination with triphasic levonorgestrel (LNg), monophasic levonorgestrel, and monophasic desogestrel (DGS), respectively, were given to 65 healthy women, n = 21-22 in each group. Blood...

  7. Assessment of the effect of phytic acid on the labeling of blood cells and plasma proteins with Technetium-99m

    Energy Technology Data Exchange (ETDEWEB)

    Lima-Filho, Guilherme L.; Freitas, Rosimeire S.; Moreno, Silvana R.F.; Boasquevisque, Edson M.; Bernardo-Filho, Mario [Universidade do Estado, Rio de Janeiro, RJ (Brazil). Inst. de Biologia. Dept. de Biofisica e Biometria]. E-mail: gllf@hotmail.com; Lima, Glaydes M.T. [Pernambuco Univ., Recife, PE (Brazil). Hospital das Clinicas; Catanho, Maria T.J.A. [Pernambuco Univ., Recife, PE (Brazil). Dept. de Biofisica e Radiobiologia

    2002-07-01

    Blood elements labeled with technetium-99m ({sup 99m} Tc) have been used in various procedures in nuclear medicine. We have investigated if phytic acid (PHY) could alter the labeling of blood elements with {sup 99m} Tc. Blood was incubated with different concentrations of PHY. Stannous chloride and {sup 99m}Tc, as sodium pertechnetate, were added. Blood was centrifuged and plasma (P) and blood cell (BC) were isolated. Samples of P and BC were also precipitated with trichloroacetic acid and centrifuged, and insoluble (IF) and soluble (SF) fractions were separated. The percentages of radioactivity (%ATI) in BC, IF-P and IF-BC were calculated. The %ATI decreased significantly (p < 0.05) in BC (95.08 {+-}1.94 to 80.68 {+-} 3.35), in IF-P (74.42 {+-}4.50 to 39.94{+-} 5.51) and in IF-BC (89.91{+-} 3.91 to 79.54 {+-} 5.42) in presence of PHY. These results suggest that the chelating property of PHY can modify the labeling of the BC, although other effects of PHY could be responsible. As PHY is found in many food and it could alter the labeling of blood elements with {sup 99m} Tc with possible undesirable effects, it is relevant to verify the necessity to repeat the examination and to evaluate the increase of the radiation dose to the patient. (author)

  8. C - reactive protein and chitinase 3-like protein 1 as biomarkers of spatial redistribution of retinal blood vessels on digital retinal photography in patients with diabetic retinopathy

    Directory of Open Access Journals (Sweden)

    Sonja Predrag Cekic

    2014-08-01

    Full Text Available The aim of the study was to investegate the correlation between the levels of CRP and YKL-40 in blood samples with morphometric parameters of retinal blood vessels in patients with diabetic retinopathy.Blood laboratory examination of 90 patients included the measurement of glycemia, HbA1C, total cholesterol, LDL-C, HDL-C, triglycerides and CRP. Levels of YKL-40 were detected and measured in serum by ELISA (Micro VueYKL-40 EIA Kit, Quidel Corporation, San Diego, USA.Morphmetric analysis was performed with ImageJ software (http://rsbweb.nih.gov/ij/ for digital retinal photography. We measured the number, diameter of retinal blood vessels in five different parts concentric to the optic disc. Differences between the morphometric parameters and the blood test analysis results were evaluated using the Student’s t – test. One Way ANOVA was used to establish the significance of differences.CRP and YKL-40 levels were moderately higher in the group of patients with severe diabetic retinopathy. Levels of YKL-40 correlated positively with diameter and negatively with number of retinal blood vessels. The average number of the blood vessels per retinal zone was significantly higher in the group of patients with mild non-proliferative diabetic retinopathy than in the group with severe form in the optic disc and all five retinal zones. The average outer diameter of the evaluated retinal zones and optic disc vessels was significantly higher in the group with severe compared to the group with mild diabetic retinopathy.Morphological analysis of the retinal vessels on digital fundus photography and correlation with YKL-40 may be valuable for the follow-up of diabetic retinopathy.

  9. Ethnographische Filme und die Darstellung von Frauen

    Directory of Open Access Journals (Sweden)

    Judith Keilbach

    2002-03-01

    Full Text Available In dieser Filmographie werden ethnographische und koloniale Filme aus dem Bestand des Nederlands Filmmuseum kommentiert, die für die Frage nach der Darstellung von Frauen und Geschlechterverhältnissen von Interesse sind.

  10. Investigation of Fasciculation and Elongation Protein ζ-1 (FEZ1 in Peripheral Blood Reveals Differences in Gene Expression in Patients with Schizophrenia

    Directory of Open Access Journals (Sweden)

    Vachev T.I.

    2015-06-01

    Full Text Available Schizophrenia (SZ is a chronic neuropsychiatric disorder characterized by affective, neuromorphological and cognitive impairment, deteriorated social functioning and psychosis with underlying molecular abnormalities, including gene expression changes. Observations have suggested that fasciculation and elongation protein ζ-1 (FEZ1 may be implicated in the pathogenesis of schizophrenia. Nevertheless, our current knowledge of the expression of FEZ1 in peripheral blood of schizophrenia patients remains unclear. The purpose of this study was to identify the characteristic gene expression patterns of FEZ1 in peripheral blood samples from schizophrenia patients. We performed quantitative reverse-transcriptase (qRT-PCR analysis using peripheral blood from drug-free schizophrenia patients (n = 29 and age and gender-matched general population controls (n = 24. For the identification of FEZ1 gene expression patterns, we applied a comparative threshold cycle (CT method. A statistically significant difference of FEZ1 mRNA level was revealed in schizophrenia subjects compared to healthy controls (p = 0.0034. To the best of our knowledge, this study is the first describing a down-regulation of FEZ1 gene expression in peripheral blood of patients with schizophrenia. Our results suggested a possible functional role of FEZ1 in the pathogenesis of schizophrenia and confirmed the utility of peripheral blood samples for molecular profiling of psychiatric disorders including schizophrenia. The current study describes FEZ1 gene expression changes in peripheral blood of patients with schizophrenia with significantly down-regulation of FEZ1 mRNA. Thus, our results provide support for a model of SZ pathogenesis that includes the effects of FEZ1 expression.

  11. Influence of histologic chorioamnionitis and funisitis on the level of peripheral blood C-reactive protein at birth in preterm infants

    Directory of Open Access Journals (Sweden)

    Do-Hyun Kim

    2010-01-01

    Full Text Available Purpose : The objective of this study is to determine the change of C-reactive protein (CRP levels in the peripheral blood of preterm infants at birth according to the stage of intrauterine inflammation. Methods : A total of 187 infants (&lt;32 weeks of gestation were divided into a “no histologic chorioamnionitis” [HCAM (-, n=85] group and a “histologic chorioamnionitis” [HCAM (+, n=102] group according to placental pathologic findings. Furthermore, the HCAM (+ group was subdivided into a “funisitis” [F (+, n=49] group and a “no funisitis” [F (-, n=53] group and also into a “funisitis/amnionitis” [FA (+, n=58] group and an “isolated chorio-deciduitis” [FA (-, n=44] group. High-sensitivity CRP levels in the peripheral blood at birth were measured. Results : Peripheral blood CRP levels were significantly higher in the HCAM (+, F (+, F (-, and FA (+ groups than in the HCAM (- group, but were not significantly different between the FA (- and HCAM (- groups. In addition, peripheral blood CRP levels were significantly higher in the F (+ and FA (+ groups than in the F (- and FA (- groups, respectively. For identification of amnionitis or funisitis, a cut-off value of 0.02 mg/dL was chosen. Clinical chorioamnionitis, proven early onset sepsis, histologic chorioamnionitis, and funisitis had higher incidences in infants with peripheral blood CRP levels higher than 0.02 mg/dL. Conclusion : The present study shows that peripheral blood CRP levels at birth in preterm infants born before 32 weeks’ gestation is significantly increased in amnionitis or funisitis and might reflect the progress of histologic chorioamnionitis.

  12. Einfluss von Saatzeit und Strohmulch auf Blattlausbesiedelung und Viruserkrankungen in Fababohnen (Vicia faba L.)

    OpenAIRE

    Saucke, Helmut; Juergens, Monique; Döring, Thomas Felix; Fittje, Susanne; Lesemann, Dietrich-Eckhard; Vetten, Heinrich-Josef

    2008-01-01

    Fababohnen (Vicia faba L.) nehmen in der Ökologischen Landwirtschaft als Protein- und N-Quelle eine wichtige Stellung in der Fruchtfolge ein (Lampkin 1994; Müller & von Fragstein und Niemsdorff 2006). Als ertragslimitierende Faktoren spielen neben Wasserknappheit auch Schaderreger wie Insekten, Pilze und verschiedene Viruserkrankungen (Cockbain 1983) eine wichtige Rolle. Virusbedingte Ertragseinbußen wurden von Schmidt (1984) in konventionellen Fababohnen Ostdeutschlands auf jährlich ca. 8% g...

  13. Herstellung von hohlen mineralischen Strukturen auf der Basis von Hefezellen

    OpenAIRE

    Weinzierl, Daniel

    2009-01-01

    Ziel der Arbeit war die Erzeugung von hohlen Pigmenten durch die Bildung einer mineralischen, hohlen Struktur an der Oberfläche von Hefezellen. Dafür sollten großtechnisch herstellbare Hefen so modifiziert werden, dass sie als kleinzellige Kristallisationskerne für die Herstellung der mineralisierten Pigmenthohl-körper dienen. Somit sollten teure Mineralien auf günstige, hohle Strukturen aufgebracht werden, um kostengünstige Hohlpigmente herstellen zu können. Die hergestellten hohlen Pi...

  14. Dietary supplementation with dried chicory root triggers changes in the blood serum proteins engaged in the clotting process and the innate immune response in growing pigs.

    Science.gov (United States)

    Lepczynski, A; Herosimczyk, A; Ozgo, M; Skomial, J; Taciak, M; Barszcz, M; Berezecka, N

    2015-02-01

    The aim of the study was to characterize the systemic immune and metabolic alterations in the blood serum of growing pigs in response to a dietary supplementation with 4% of dried chicory roots. This was achieved by examining the influence of the experimental diet on serum protein changes especially these related with immunology and lipid metabolism. Serum proteins with the isoelectric point ranging from pH 3.0 to 10.0 were separated using high resolution two-dimensional electrophoresis. As a result, we found that experimental diet triggered significant changes in 37 protein spots. Of these, 14 were up-regulated, whereas 23 showed down-regulation. Of 37 significantly altered protein spots, 24 were successfully identified, representing 14 distinct gene products. Implementation of the dried chicory roots into the diet of growing pigs caused a significant down-regulation of apolipoprotein C-II complement component C6, C-reactive protein, CD14 antigen, C4b binding protein α and β chains, and fibrinogen. Piglets fed experimental diet had similar IgA, IgG and IgM concentrations, although the level of IgM tended to be lower compared to the control group. It is concluded that diet supplemented with 4% of dried chicory root may exert anti-inflammatory properties and affect lipid metabolism in growing pigs. PMID:25716964

  15. Acute effects of pea protein and hull fibre alone and combined on blood glucose, appetite, and food intake in healthy young men--a randomized crossover trial.

    Science.gov (United States)

    Mollard, Rebecca C; Luhovyy, Bohdan L; Smith, Christopher; Anderson, G Harvey

    2014-12-01

    Whether pulse components can be used as value-added ingredients in foods formulated for blood glucose (BG) and food intake (FI) control requires investigation. The objective of this study was to examine of the effects of pea components on FI at an ad libitum meal, as well as appetite and BG responses before and after the meal. In a repeated-measures crossover trial, men (n = 15) randomly consumed (i) pea hull fibre (7 g), (ii) pea protein (10 g), (iii) pea protein (10 g) plus hull fibre (7 g), (iv) yellow peas (406 g), and (v) control. Pea hull fibre and protein were served with tomato sauce and noodles, while yellow peas were served with tomato sauce. Control was noodles and tomato sauce. FI was measured at a pizza meal (135 min). Appetite and BG were measured pre-pizza (0-135 min) and post-pizza (155-215 min). Protein plus fibre and yellow peas led to lower pre-pizza BG area under the curve compared with fibre and control. At 30 min, BG was lower after protein plus fibre and yellow peas compared with fibre and control, whereas at 45 and 75 min, protein plus fibre and yellow peas led to lower BG compared with fibre (p yellow peas led to lower BG compared with fibre (p pea components as value-added ingredients in foods designed to improve glycemic control. PMID:25302637

  16. Langfristige Perspektiven von Anlagen in Sachwerten

    OpenAIRE

    Bräuninger, Michael; Stiller, Silvia; Vöpel, Henning

    2009-01-01

    Die erheblichen Turbulenzen an den Finanzmärkten, die Talfahrten von Aktienkursen sowie die Veränderung der relativen Attraktivität unterschiedlicher Anlageformen beeinflussen die Investitionsentscheidungen von Anlegern. In der Studie untersucht das HWWI die Rentabilität von Investitionen in Sachwerte. Die Analyse zeigt besondere Chancen von Schifffahrts- und Immobilienmärkten. Dazu werden das globale Wirtschaftswachstum, der Globalisierungsprozess sowie die Finanzierungsformen über Fremdkapi...

  17. Bewirtschaftung und Ökobilanzierung von Kurzumtriebsplantagen

    OpenAIRE

    Burger, Frank

    2011-01-01

    Die Arbeit beschreibt den kompletten Produktionszyklus von Kurzumtriebsplantagen. Alle Maßnahmen, die zur Anlage einer solchen Kultur notwendig sind, von der Vorbereitung des Ackers, der Wahl der Umtriebszeit, der Pflanzung der Balsampappeln bis zur Rodung werden aufgezeigt. Ertragskundliche Erhebungen, die seit 18 Jahren auf bayerischen Versuchsstandorten durchgeführt wurden, geben einen Eindruck von den möglichen Biomasseleistungen. Sieben Erntesysteme zur Produktion von Holzhackschnitzeln ...

  18. Kinematische Analyse von Schreibbewegungen im Erstschreibunterricht

    OpenAIRE

    Quenzel, Irmina; Mai, Norbert

    2000-01-01

    Die Automation von Schreibbewegungen ist ein zentrales Merkmal geübten, flüssigen Schreibens. Mittels eines digitalen Schreibbrettes wurde die Schrift von 14 Grundschülern mit der von 10 routinierten erwachsenen Schreibern verglichen. Es zeigte sich, dass Kinder zu Beginn des Schreibunterrichtes bereits über die Kompetenz zu automatisierter Ausführung einfacher Schreibbewegungen verfügen, diese jedoch beim Schreiben von Buchstaben noch nicht einsetzen. Weiterhin erwies sich, dass bei der Vorg...

  19. Monitoring the changes in plasm C-reactive protein,fibrinogen and blood white cell in patients with primary hypertension combined with acute cerebral infarction

    Institute of Scientific and Technical Information of China (English)

    Yuanfei Deng; Juan Hang; Yane Chen

    2006-01-01

    BACKGROUND: Inflammatow reaction and the increased level of its accompanying active protein play an important role in the occurrence and development of cerebral infarction. C-reactive protein, fibrinogen and white blood cell, as the monitoring index of inflammatory reaction, are very important in the occurrence and development of acute cerebral infarction.OBJECTIVE: To make a comparison between patients with primary hypertension accompanied with acute cerebral infarction and with simple primary hypertension by observing the changes in plasma C-reactive protein and fibrinogen levels as well as white blood cell and differential counts and analyzing their significances.DESIGN : Controlled observation.SETTTNG: Ward Building for VIP, Shenzhen Hospital, Peking University.PARTICIPANTS: Totally 133 patients with primary hypertension were selected from Ward Building for VIP,Shenzhen Hospital, Peking University during September 2003 to September 2005. The diagnostic criteda were based on the hypertension diagnosis criteria formulated by the 7th World Health Organization-lnternational Society of Hypertension Guidelines (WHO-ISH) in 1998. The informed consents were obtained from all the participants. The involved patients were assigned into two groups: primary hypertension group, in which, there were 65 patients with primary hypertension ( degree 2), including 42 males and 23 females,with mean age of (61 ±14)years and mean blood pressure of (162.7±6.8)/(94.2±8.4) mm Hg(1 mm Hg =0.133 kPa), and primary hypertension combined with cerebral infarction group, in which, there were 68 patients with primary hypertension combined with cerebral infarction ( meeting the diagnostic criteria formulated in the 4th National Cerebrovascular Diseases Meeting in 1995 and diagnosed by skull CT or MRI to exclude the patients with lacunar infarction), including 42 males and 26 females, with mean age of (56±15)years and mean blood pressure of (176.4±9.2)/(96.3±9.7) mm Hg.METHODS: Plasm C

  20. Approximate double commutants in von Neumann algebras

    OpenAIRE

    Hadwin, Don

    2011-01-01

    Richard Kadison showed that not every commutative von Neumann subalgebra of a factor von Neumann algebra is equal to its relative double commutant. We prove that every commutative C*-subalgebra of a centrally prime C*-algebra $B$ equals its relative approximate double commutant. If $B$ is a von Neumann algebra, there is a related distance formula.

  1. Hellmut von Glahn 1929 – 2008

    OpenAIRE

    Janiesch, Peter

    2010-01-01

    Nachruf Hellmut von Glahn, Gründungsmitglied und von 1979 bis 2006 Vorsitzender des Vereins zur Förderung naturkundlicher Untersuchungen in Nordwestdeutschland e. V., der seit 1976 das Erscheinen der Zeitschrift DROSERA maßgeblich fördert, ist nach schwerer Krankheit am 25.10.2008 im Alter von 80 Jahren gestorben.

  2. Fachspezifische Vermittlung von Informationskompetenz an Biologen

    OpenAIRE

    Kristina Hartmann

    2013-01-01

    Die Vermittlung von Informationskompetenz ist an vielen Hochschulbibliotheken etabliert. Dabei existieren sowohl fachübergreifende als auch fachspezifische Vermittlungskonzepte. Dieser Artikel leistet einen Beitrag zur fachspezifischen Vermittlung von Informationskompetenz an Biologen. Hierfür wurden mittels einer Umfrage die aktuellen Angebote der deutschen Hochschulbibliotheken im Fach Biologie und ihre Verankerung im Fachbereich ermittelt. Der Schwerpunkt in der Vermittlung von Inf...

  3. Online monitoring of immunoaffinity-based depletion of high-abundance blood proteins by UV spectrophotometry using enhanced green fluorescence protein and FITC-labeled human serum albumin

    Directory of Open Access Journals (Sweden)

    Yu Hyeong

    2010-12-01

    Full Text Available Abstract Background The removal of high-abundance proteins from plasma is an efficient approach to investigating flow-through proteins for biomarker discovery studies. Most depletion methods are based on multiple immunoaffinity methods available commercially including LC columns and spin columns. Despite its usefulness, high-abundance depletion has an intrinsic problem, the sponge effect, which should be assessed during depletion experiments. Concurrently, the yield of depletion of high-abundance proteins must be monitored during the use of the depletion column. To date, there is no reasonable technique for measuring the recovery of flow-through proteins after depletion and assessing the capacity for capture of high-abundance proteins. Results In this study, we developed a method of measuring recovery yields of a multiple affinity removal system column easily and rapidly using enhanced green fluorescence protein as an indicator of flow-through proteins. Also, we monitored the capture efficiency through depletion of a high-abundance protein, albumin labeled with fluorescein isothiocyanate. Conclusion This simple method can be applied easily to common high-abundance protein depletion methods, effectively reducing experimental variations in biomarker discovery studies.

  4. Tissue protein nitration and peripheral blood endotoxin activity are indicative of the severity of systemic organ compromise in naturally-occurring clinical cases of bacterial mastitis in Holstein dairy cows

    Science.gov (United States)

    The objective of this survey study was to determine a relationship between the intensity of tissue protein tyrosine nitration measured in samples of mammary gland, liver, pancreas and lung compared to estimated blood endotoxin (LPS) activity. Blood was collected from nine multiparous Holstein cows...

  5. A phosphatase activity present in peripheral blood myeloid cells of chronic myelogenous leukemia patients but not normal individuals alters nuclear protein binding to transcriptional enhancers of interferon-inducible genes.

    OpenAIRE

    Seong, D C; Sims, S.; Johnson, E.; Howard, O M; Reiter, B; Hester, J; Talpaz, M; Kantarjian, H; Deisseroth, A

    1990-01-01

    Cytoplasmic protein from peripheral blood myeloid cells of chronic myelogenous leukemia (CML) patients altered the electrophoretic mobility of complexes formed between nuclear proteins and interferon-inducible transcriptional enhancers. Immature myeloid marrow cells (blasts and promyelocytes) have a higher level of this activity than do mature myeloid marrow cells (bands and polys). This activity, which is not detectable in the peripheral blood cells of normal individuals, is at least 50-fold...

  6. The effects of DNA intercalators on chromatin of chicken red blood cells——differential extraction on nonhistone proteins

    Institute of Scientific and Technical Information of China (English)

    WangFan; ZhuJingde

    1990-01-01

    Taking advantage of the effects on DNA secondary structure of two DNA-intercalators,ethidium bromide and chloroquine,we used each of them to treat nuclei from both mature erythrocytes and reticulocytes of chicken,as an alternative approach to study the relationships between DNA secondary structure,nuclear proteins and chromatin structure.We presented results of differential extraction of nuclear proteins from nuclei with DNA-intercalators,as well as preliminary characterization of these proteins.A 45kd protein is the major component in fractions extracted by both intercalators from nuclei from either mature erythrocytes or reticulocytes and seems to be a DNA-binding protein.Furthermore,from current concepts of functional aspects of DNA conformation and structural heterogeneity in chromatin and nuclear proteins,we have discussed both the significance of our results as well as technical aspects of this approach.

  7. Marked increase in rat red blood cell membrane protein glycosylation by one-month treatment with a cafeteria diet.

    OpenAIRE

    Oliva, Laia; Baron, Cristian; Fernández-López, José-Antonio; Remesar, Xavier; Alemany, Marià

    2015-01-01

    Background and Objectives. Glucose, an aldose, spontaneously reacts with protein amino acids yielding glycosylated proteins. The compounds may reorganize to produce advanced glycosylation products, which regulatory importance is increasingly being recognized. Protein glycosylation is produced without the direct intervention of enzymes and results in the loss of function. Glycosylated plasma albumin, and glycosylated haemoglobin are currently used as index of mean plasma glucose levels, since ...

  8. Preclinical investigation of the pharmacokinetics, metabolism, and protein and red blood cell binding of DRDE-07: a prophylactic agent against sulphur mustard

    Directory of Open Access Journals (Sweden)

    Pankaj Verma

    2014-10-01

    Full Text Available DRDE-07, a newly synthesized amifostine analog currently under clinical investigation in a phase I trial, is a potent antidote against sulfur mustard toxicity. The purpose of this research was to evaluate the pharmacokinetic profile of DRDE-07 in female Swiss Albino mice after a single oral dose of 400 or 600 mg/kg. The physicochemical properties of DRDE-07, including solubility, pKa, Log P, plasma protein binding and plasma/blood partitioning, were determined to support the pharmacokinetic characterization. DRDE-07 concentration was determined by an HPLC-UV method. The profile of plasma concentration versus time was analyzed using a non-compartmental model. Plasma protein binding was assessed using ultrafiltration. DRDE-07 appeared rapidly in plasma after oral administration with peak plasma levels (Cmax observed in less than 15 min. There was a rapid decline in the plasma levels followed by a smaller second peak about 90 min after dosing. The plasma protein binding of DRDE-07 was found to be less than 25% at all concentrations studied. Plasma clearance of DRDE-07 is expected to be ~1.5 fold higher than the blood clearance of DRDE-07. The probable metabolite of DRDE-07 was identified as phenyl-S-ethyl amine.

  9. Ultraproducts of von Neumann algebras

    DEFF Research Database (Denmark)

    Ando, Hiroshi; Haagerup, Uffe

    2014-01-01

    We study several notions of ultraproducts of von Neumann algebras from a unified viewpoint. In particular, we show that for a sigma-finite von Neumann algebra M  , the ultraproduct MωMω introduced by Ocneanu is a corner of the ultraproduct ∏ωM∏ωM introduced by Groh and Raynaud. Using this...... the ultrapower MωMω of a Type III0 factor is never a factor. Moreover we settle in the affirmative a recent problem by Ueda about the connection between the relative commutant of M   in MωMω and Connes' asymptotic centralizer algebra MωMω....

  10. Direktsaat von Mais im Biolandbau

    OpenAIRE

    Dierauer, Hansueli; Hegglin, Django; Böhler, Daniel

    2014-01-01

    Der Mais ist für die Fütterung von Wieder- als auch Nichtwiederkäuer eine sehr wertvolle Pflanze. Im Biolandbau wird für den Maisanbau aufgrund der einfacheren Unkrautregulierung standardmässig der Pflug verwendet. Bezüglich Bodenerosion, Verdichtung und Abschwemmung ist aber gerade beim Maisanbau der Pflugeinsatz nicht optimal. Die Direktsaat von Mais löst diese Probleme weitgehend und erhöht auch im Biolandbau die Akzeptanz, da auf dem geschlossenen Boden auch die Ernte bodenschonender durc...

  11. Expression of peptide fragments from proADM and involvement of mitogen-activated protein kinase signaling pathways in pulmonary remodeling induced by high pulmonary blood flow.

    Science.gov (United States)

    Li, Wei; Guo, Aili; Wang, Lijuan; Kong, Qingyu; Wang, Rong; Han, Li; Zhao, Cuifen

    2016-01-01

    Pulmonary arterial hypertension (PAH) is a life-threatening disease characterized by progressive pulmonary arterial remodeling and right ventricular failure. Despite recent advances in pathophysiological mechanism exploration and new therapeutic approaches, PAH remains a challenging condition. In this study, we investigated the roles of the peptide fragments from proadrenomedullin (proADM) such as adrenomedullin (ADM), adrenotensin (ADT), and proadrenomedullin N-terminal 20 peptide (PAMP) during pulmonary remodeling caused by high pulmonary blood flow, and probed the possible involvement of mitogen-activated protein kinase (MAPK) signal transduction pathways. Sixteen rat models of PAH were artificially established by surgically connecting the left common carotid artery to the external jugular vein. We subcutaneously injected an extracellular signal-regulated protein kinase (ERK1/2) inhibitor, PD98059, in eight rats, treated another eight rats with an equal volume of saline. Eight rats without connections served as the control group. We observed that mRNA expression levels of ADM, stress-activated protein kinase (SAPK), and ERK1/2 were significantly elevated in the shunted rats; furthermore, ERK1/2 levels were significantly inhibited by PD98059. Protein levels of ADM, PAMP, p-SAPK, and p-ERK1/2 were significantly higher ADT was lower, and p-p38 remained unchanged in the rat models compared with the controls. However, the protein expression of both ADM and p-ERK1/2 was significantly inhibited by PD98059. Our results suggest that levels of ADM, ADT, and PAMP respond to pulmonary remodeling, and that activation of the SAPK and ERK1/2 signaling pathways is involved in pulmonary hypertension and artery remodeling caused by high pulmonary blood flow. PMID:25990643

  12. STUDY OF ERYTHROCYTE SEDIMENTATION RATE,SERUM TOTAL PROTEIN, SERUM ALBUMIN,SERUM GLOBULIN AND RED BLOOD CELL COUNT IN DIFFERENT PHASES OF MENSTRUAL CYCLE

    Directory of Open Access Journals (Sweden)

    Shilpa

    2014-09-01

    Full Text Available : BACKGROUND: Menstruation is a phenomenon unique to females and nearly universal experience in women's lives and is poorly understood. It is characterized by co-ordinate sequence of hormonal changes but the changes that occur in the distribution of hematological parameters, biochemical parameters have not been clearly established. AIMS: To compare Erythrocyte Sedimentation Rate (ESR, Serum total protein, Serum albumin, Serum globulin and Red Blood Cell count (RBC in different phases of menstrual cycle. MATERIALS & METHODS: The present study was carried out on 100 healthy female medical students in the age group of 18 to 23years with normal menstrual cycle. E.S.R, Serum total protein, Serum albumin, Serum globulin and RBC count were studied on 2nd day MP(Menstrual Phase,11th day PP(Proliferative Phase, 22ndday SP(Secretory Phase of menstrual cycle using Wintergreen’s method, semi auto analyzer Erba chem-7(BCG Dye method for serum proteins, Hemo Auto analyzer SYSMEX KX-21 respectively. Statistical analysis was done using SPSS 17.0 Software. To compare means of two independent groups, student’s t- test for independent samples was used. RESULTS: The study revealed that ESR was significantly (P<0.01 higher in menstrual phase compared to proliferative phase. Serum total protein was significantly increased in Secretory Phase (SP compared to Menstrual Phase (MP (P<0.05. Serum albumin was significantly (P<0.05 increased in proliferative phase compared to secretory phase and menstrual phase. Serum globulin was significantly increased in SP compared to PP (P<0.05. Red blood cell count showed no significant changes during various phases of menstrual cycle. CONCLUSION: In the present study we found significantly low level of serum albumin and decreased RBC count though not significant increases rouleaux formation thus increasing ESR in the menstrual phase but serum globulin and serum total protein showed increase in secretory phase which does not

  13. Binding of complement regulators factor H and C4b binding protein to group A streptococcal strains isolated from tonsillar tissue and blood.

    Science.gov (United States)

    Suvilehto, Jari; Jarva, Hanna; Seppänen, Mikko; Siljander, Tuula; Vuopio-Varkila, Jaana; Meri, Seppo

    2008-06-01

    Group A streptococcus (GAS) is the most common pathogen causing bacterial pharyngitis. We isolated streptococcal strains from tonsils removed from patients with tonsillar disease (n=202) and studied their ability to bind the complement regulators factor H (FH) and C4b binding protein (C4BP) using 125 I-labeled proteins. Blood isolates of GAS (n=10) were obtained from patients with bacteraemia. Streptococci were isolated from 21% of the tonsillitis patients. The emm and T types of the GAS strains were determined. Of the 26 GAS strains studied, only six could bind FH and/or C4BP above the threshold levels. The fraction of the offered radioactive protein bound ranged between 6-12% for FH and 19-56% for C4BP. The clinical course of the tonsillar disease was not related to the binding of FH or C4BP by GAS. The binding strains were mostly of the T4M4 or T28M28 type. From the invasive strains (n=10), three bound FH (binding level: 8-11%) and two C4BP (36-39%). The binding correlated only partially to M-protein (emm) type suggesting that the binding was not exclusively due to M-protein. The results indicate that complement regulator binding by GAS is only partially related to pathogenicity and not a universal property of all group A streptococci. PMID:18538613

  14. Mechanismen zur Entstehung von Autoimmunität im Zentralnervensystem

    OpenAIRE

    Amend, Bastian

    2008-01-01

    Die experimentelle autoimmune Enzephalomyelitis (EAE) gilt als Modellerkrankung der Multiplen Sklerose und stellt eine Forschungsgrundlage zur Aufklärung von Me-chanismen organspezifischer Autoimmunerkrankungen dar. SJL-Mäuse entwickeln nach Immunisierung mit einem Peptid des Proteolipid Protein (PLP139-151), einem Bestandteil des Myelins im zentralen Nervensystem (ZNS), eine chronische EAE, die dem Verlauf einer schubförmig-remittierenden Multiplen Sklerose nahe kommt. Dies beruht auf der Ak...

  15. Heat shock protein 72 normothermic ischemia, and the impact of congested portal blood reperfusion on rat liver

    Institute of Scientific and Technical Information of China (English)

    Chao Liu Dai; Zhen Long Xia; Makoto Kume; Yuzo Yamamoto; Kazuhiko Yamagami; Nobuhiro Ozaki; Yoshio Yamaoka

    2001-01-01

    @@INTRODUCTION From the technical aspect of liver surgery ,control of bleeding during hepatic parenchymal resection is one of the most important procedures in hepatectomy .Pringle,s maneuver ,a temporary cross-clamping of the hepatoduodnal ligament ,has often been used for this purpose[1],This is the simplest and userul technique to reduce intraoperative blood loss .

  16. Polymer brushes showing non-fouling in blood plasma challenge the currently accepted design of protein resistant surfaces

    Czech Academy of Sciences Publication Activity Database

    Rodriguez-Emmenegger, Cesar Adolfo; Brynda, Eduard; Riedel, Tomáš; Houska, Milan; Šubr, Vladimír; Bologna Alles, A.; Hasan, E.; Gautrot, J. E.; Huck, W. T. S.

    2011-01-01

    Roč. 32, č. 13 (2011), s. 952-957. ISSN 1022-1336 R&D Projects: GA AV ČR KAN200670701; GA AV ČR IAAX00500803 Institutional research plan: CEZ:AV0Z40500505 Keywords : biosensors * blood plasma * HPMA Subject RIV: CD - Macromolecular Chemistry Impact factor: 4.596, year: 2011

  17. The onset of the progression of acute phase response mechanisms induced by extreme impacts can be followed by the decrease in blood levels of positive acute phase proteins.

    Science.gov (United States)

    Larina, Olga; Bekker, Anna

    Studies performed at space flights and earth-based simulation models detected the plasma indices of acute phase reaction (APR), i.e. the increase of APR cytokine mediators and alterations in the production of blood acute phase proteins (APP) at the initial stages of adaptation to altered gravity conditions. Acute phase response is the principal constituent of the functional activity of innate immunity system. Changes in plasma APPs contents are considered to serve the restoration of homeostasis state. According to trends of their concentration shifts at the evolving of acute phase reaction APPs are denoted as positive, neutral, or negative. Plasma concentrations of positive acute phase proteins α1-acid glycoprotein (α1-AGP), α1-antitrypsin (α1-AT), and neutral α2-macroglobulin (α2-M) were measured in human study at 12-hour antiorthostatic position (AOP) with 15° head down tilt and hypoxia experiments at 14% oxygen in pressure chamber. Both of these impacts were shown to produce alterations in the APP levels indicative for acute phase response. Nevertheless, in AOP experiment noticeable decrease in α1-AGP concentration occurred by hour 12, and even more pronounced decline of α1-AGP and α1-AT were found on hypoxia hours 12 and 36. Acute phase proteins α1-AGP and α2-M possess the features of proteinase inhibitors. This function is implemented by the formation of complexes with the molecules of proteolytic enzymes which subsequently are removed from the blood flow. Transient decrease in plasma concentrations of protease inhibitors on early phases of APR development was reported to result from the growth of plasma protease activity due to cathepsin release from activated leukocytes, which had not yet been compensated by enhanced APP synthesis. Being a carrier protein for positively charged and neutral substances, α1-AGP shows pronounced elevation in its blood content during APR development. As assumed, it is required for the transportation of the increased

  18. A Paleolithic diet confers higher insulin sensitivity, lower C-reactive protein and lower blood pressure than a cereal-based diet in domestic pigs

    Science.gov (United States)

    Jönsson, Tommy; Ahrén, Bo; Pacini, Giovanni; Sundler, Frank; Wierup, Nils; Steen, Stig; Sjöberg, Trygve; Ugander, Martin; Frostegård, Johan; Göransson, Leif; Lindeberg, Staffan

    2006-01-01

    Background A Paleolithic diet has been suggested to be more in concordance with human evolutionary legacy than a cereal based diet. This might explain the lower incidence among hunter-gatherers of diseases of affluence such as type 2 diabetes, obesity and cardiovascular disease. The aim of this study was to experimentally study the long-term effect of a Paleolithic diet on risk factors for these diseases in domestic pigs. We examined glucose tolerance, post-challenge insulin response, plasma C-reactive protein and blood pressure after 15 months on Paleolithic diet in comparison with a cereal based swine feed. Methods Upon weaning twenty-four piglets were randomly allocated either to cereal based swine feed (Cereal group) or cereal free Paleolithic diet consisting of vegetables, fruit, meat and a small amount of tubers (Paleolithic group). At 17 months of age an intravenous glucose tolerance test was performed and pancreas specimens were collected for immunohistochemistry. Group comparisons of continuous variables were made by use of the t-test. P < 0.05 was chosen for statistical significance. Simple and multivariate correlations were evaluated by use of linear regression analysis. Results At the end of the study the Paleolithic group weighed 22% less and had 43% lower subcutaneous fat thickness at mid sternum. No significant difference was seen in fasting glucose between groups. Dynamic insulin sensitivity was significantly higher (p = 0.004) and the insulin response was significantly lower in the Paleolithic group (p = 0.001). The geometric mean of C-reactive protein was 82% lower (p = 0.0007) and intra-arterial diastolic blood pressure was 13% lower in the Paleolithic group (p = 0.007). In evaluations of multivariate correlations, diet emerged as the strongest explanatory variable for the variations in dynamic insulin sensitivity, insulin response, C-reactive protein and diastolic blood pressure when compared to other relevant variables such as weight and

  19. Expression of Iron-Related Proteins at the Neurovascular Unit Supports Reduction and Reoxidation of Iron for Transport Through the Blood-Brain Barrier

    DEFF Research Database (Denmark)

    Burkhart, Annette; Skjørringe, Tina; Johnsen, Kasper Bendix;

    2015-01-01

    The mechanisms for iron transport through the blood-brain barrier (BBB) remain a controversy. We analyzed for expression of mRNA and proteins involved in oxidation and transport of iron in isolated brain capillaries from dietary normal, iron-deficient, and iron-reverted rats. The expression was...... endothelial cells provide the machinery for receptor-mediated uptake of ferric iron-containing transferrin. Ferric iron can then undergo reduction to ferrous iron by ferrireductases inside endosomes followed by DMT1-mediated pumping into the cytosol and subsequently cellular export by ferroportin. The...

  20. A Paleolithic diet confers higher insulin sensitivity, lower C-reactive protein and lower blood pressure than a cereal-based diet in domestic pigs

    Directory of Open Access Journals (Sweden)

    Ugander Martin

    2006-11-01

    Full Text Available Abstract Background A Paleolithic diet has been suggested to be more in concordance with human evolutionary legacy than a cereal based diet. This might explain the lower incidence among hunter-gatherers of diseases of affluence such as type 2 diabetes, obesity and cardiovascular disease. The aim of this study was to experimentally study the long-term effect of a Paleolithic diet on risk factors for these diseases in domestic pigs. We examined glucose tolerance, post-challenge insulin response, plasma C-reactive protein and blood pressure after 15 months on Paleolithic diet in comparison with a cereal based swine feed. Methods Upon weaning twenty-four piglets were randomly allocated either to cereal based swine feed (Cereal group or cereal free Paleolithic diet consisting of vegetables, fruit, meat and a small amount of tubers (Paleolithic group. At 17 months of age an intravenous glucose tolerance test was performed and pancreas specimens were collected for immunohistochemistry. Group comparisons of continuous variables were made by use of the t-test. P Results At the end of the study the Paleolithic group weighed 22% less and had 43% lower subcutaneous fat thickness at mid sternum. No significant difference was seen in fasting glucose between groups. Dynamic insulin sensitivity was significantly higher (p = 0.004 and the insulin response was significantly lower in the Paleolithic group (p = 0.001. The geometric mean of C-reactive protein was 82% lower (p = 0.0007 and intra-arterial diastolic blood pressure was 13% lower in the Paleolithic group (p = 0.007. In evaluations of multivariate correlations, diet emerged as the strongest explanatory variable for the variations in dynamic insulin sensitivity, insulin response, C-reactive protein and diastolic blood pressure when compared to other relevant variables such as weight and subcutaneous fat thickness at mid sternum. There was no obvious immunohistochemical difference in pancreatic islets

  1. 胰酶水解猪血蛋白工艺条件的优化%Optimization of Conditions for the Hydrolysis of Porcine Blood Protein by Trypsin

    Institute of Scientific and Technical Information of China (English)

    江霞; 周成; 张瑜; 朱秋劲

    2011-01-01

    Porcine blood is a good cheap source of protein. Enzymatic hydrolysate of porcine blood protein can provide plenty of reaction substrates such as small-chain peptides and amino acids for Maillard reaction. In the present study, papain, alkaline protease, neutral protease, bromelain, trypsin, flavourzyme and protamex were compared for their effectiveness in hydrolyzing porcine blood protein, and trypsin was found to be the most suitable enzyme for further studies due to the highest degree of hydrolysis (DH). One-factor-at-a-time and orthogonal array design methods were employed to optimize process parameters for trypsin hydrolysis of porcine blood protein by. The optimal hydrolysis conditions were found to be: enzyme dosage of 0.5%, hydrolysis time of 5 h, temperature of 55 ℃, pH 8.5 and substrate concentration 7%, resulting in a DH of 38.72%.%为拓展猪血蛋白利用途径,提高猪血蛋白的水解率,得到更丰富的Maillard反应底物——小分子肽及氨基酸,采用木瓜蛋白酶、碱性蛋白酶、中性蛋白酶、菠萝蛋白酶、胰酶、风味蛋白酶、复合蛋白酶7种酶,在固定条件下分别对猪血进行水解,以水解度为指标筛选出水解度最高的酶,然后再对该酶进行从单因素试验到正交试验的水解工艺参数优化。结果显示:胰酶的水解效果最好,其最佳的水解条件是酶加量0.5%、水解时间5h、温度55℃、初始pH8.5、底物蛋白质量分数7%,通过一次水解,可使水解度达到38.72%。

  2. Low-protein vegetarian diet does not have a short-term effect on blood acid¿base status but raises oxygen consumption during submaximal cycling

    OpenAIRE

    Hietavala Enni-Maria; Puurtinen Risto; Kainulainen Heikki; Mero Antti A

    2012-01-01

    Abstract Background Acid–base balance refers to the equilibrium between acids and bases in the human body. Nutrition may affect acid–base balance and further physical performance. With the help of PRAL (potential renal acid load), a low-protein vegetarian diet (LPVD) was designed to enhance the production of bases in body. The aim of this study was to investigate if LPVD has an effect on blood acid–base status and performance during submaximal and maximal aerobic cycling. Methods Nine healthy...

  3. [Design of experimental approaches on the base of standard proteins for testing blood biopreparations and immunoperoxidase conjugates specific to human and animal immunoglobulines G].

    Science.gov (United States)

    Barsukov, A K; Barmin, A V; Kuznetsov, A I; Nesterova, O Iu; Ushnurtseva, S A; Panin, A N; Smolenskiĭ, V I; Ulasov, V I; Sviderskiĭ, V L; Khovanskikh, A E

    2009-01-01

    Using standard forms of immunoglobulin (Ig) G and albumin, we have studied electrophoretic and chromatographic profiles of samples of pharmaceutical blood biopreparation batches. The usability of standard proteins was also demonstrated by testing analytical characteristics of immunoperoxidase conjugates specific to human and animal IgG (anti-IgG IPC). In particular, we suggest an additional estimation of analytical characteristics of anti-IgG IPC by the enzyme reaction kinetics with the standard dilution which is calculated by the direct enzyme-liked immunoassay on the homologous IgG-antigen. PMID:19764621

  4. Molecular basis of human von Willebrand disease: Analysis of platelet von Willebrand factor mRNA

    International Nuclear Information System (INIS)

    von Willebrand disease (vWD), the most common inherited bleeding disorder in humans, can result from either a quantitative or a qualitative defect in the adhesive glycoprotein, von Willebrand factor (vWF). Molecular studies of vWD have been limited by the large size of the vWF gene and difficulty in obtaining the vWF mRNA from patients. By use of an adaptation of the polymerase chain reaction, vWF mRNA was amplified and sequenced from peripheral blood platelets. A silent vWF allele was identified, resulting from a cis defect in vWF mRNA transcription or processing. In two type IIA vWD patients, two different but adjacent missense mutations were identified, the locations of which may identify an important vWF functional domain. Expression in heterologous cells of recombinant vWF containing one of these latter mutations reproduced the characteristic structural abnormality seen in type IIA vWD plasma

  5. Methyl-binding domain protein-based DNA isolation from human blood serum combines DNA analyses and serum-autoantibody testing

    Directory of Open Access Journals (Sweden)

    Jungbauer Christof

    2011-09-01

    Full Text Available Abstract Background Circulating cell free DNA in serum as well as serum-autoantibodies and the serum proteome have great potential to contribute to early cancer diagnostics via non invasive blood tests. However, most DNA preparation protocols destroy the protein fraction and therefore do not allow subsequent protein analyses. In this study a novel approach based on methyl binding domain protein (MBD is described to overcome the technical difficulties of combining DNA and protein analysis out of one single serum sample. Methods Serum or plasma samples from 98 control individuals and 54 breast cancer patients were evaluated upon silica membrane- or MBD affinity-based DNA isolation via qPCR targeting potential DNA methylation markers as well as by protein-microarrays for tumor-autoantibody testing. Results In control individuals, an average DNA level of 22.8 ± 25.7 ng/ml was detected applying the silica membrane based protocol and 8.5 ± 7.5 ng/ml using the MBD-approach, both values strongly dependent on the serum sample preparation methods used. In contrast to malignant and benign tumor serum samples, cell free DNA concentrations were significantly elevated in sera of metastasizing breast cancer patients. Technical evaluation revealed that serum upon MBD-based DNA isolation is suitable for protein-array analyses when data are consistent to untreated serum samples. Conclusion MBD affinity purification allows DNA isolations under native conditions retaining the protein function, thus for example enabling combined analyses of DNA methylation and autoantigene-profiles from the same serum sample and thereby improving minimal invasive diagnostics.

  6. von Hippel-Lindau disease

    DEFF Research Database (Denmark)

    Binderup, Marie Louise Mølgaard; Gimsing, Steen; Kosteljanetz, Michael;

    2013-01-01

    Endolymphatic sac tumours (ELSTs) of the inner ear occur in 16% of patients with the hereditary tumor syndrome von Hippel-Lindau disease (vHL). ELSTs of all sizes can cause irreversible hearing loss which can, however, be prevented through early diagnosis and treatment. We aim to emphasize the ch...

  7. Von Krahl ON! / Terje Metsavas

    Index Scriptorium Estoniae

    Metsavas, Terje

    2008-01-01

    Madli Pesti ja Terje Metsavas kohtusid Von Krahli teatri tehnilise meeskonnaga teatri black-box-saalis. Teatri tehniline meeskond on tehnikadirektor Enar Tarmo, video- ja multimeediaga tegelev Lauri Sepp, tehnikud Janno "John" Jaanus, Oliver Kulpsoo ja Allan Räim. Selles, kuidas sünnivad "tehnikaimed" ja kuidas lahendatakse lavastuslikke projekte

  8. [Friedrich Ludwig von Maydelli pildid Baltimaade ajaloost = Friedrich Ludwig von Maydells Baltische Geschichte in Bildern = Friedrich Ludwig von Maydell ́s Baltic history in images] / Ulrike Plath

    Index Scriptorium Estoniae

    Plath, Ulrike, 1972-

    2014-01-01

    Arvustus: Friedrich Ludwig von Maydelli pildid Baltimaade ajaloost = Friedrich Ludwig von Maydells Baltische Geschichte in Bildern = Friedrich Ludwig von Maydell ́s Baltic history in images. Eesti kunstimuuseum, Kadrioru kunstimuuseum. Tallinn 2013

  9. Online monitoring of immunoaffinity-based depletion of high-abundance blood proteins by UV spectrophotometry using enhanced green fluorescence protein and FITC-labeled human serum albumin

    OpenAIRE

    Yu Hyeong; Kim Byungwook; Kim Hyunsoo; Min Hophil; Yu Jiyoung; Kim Kyunggon; Kim Youngsoo

    2010-01-01

    Abstract Background The removal of high-abundance proteins from plasma is an efficient approach to investigating flow-through proteins for biomarker discovery studies. Most depletion methods are based on multiple immunoaffinity methods available commercially including LC columns and spin columns. Despite its usefulness, high-abundance depletion has an intrinsic problem, the sponge effect, which should be assessed during depletion experiments. Concurrently, the yield of depletion of high-abund...

  10. Triton X-114 cloud point extraction to subfractionate blood plasma proteins for two-dimensional gel electrophoresis

    DEFF Research Database (Denmark)

    Jessen, Flemming; Wulff, Tune

    2015-01-01

    A simple and reproducible procedure for enrichment of a plasma protein subfraction suitable for two-dimensional polyacrylamide gel electrophoresis (2DE) was developed, using a Triton X-114-based cloud point extraction (CPE). Appropriate conditions for such a CPE procedure were found by SDS-PAGE to......-sterol acyltransferase, serum amyloid A, and serum paraoxonase/arylesterase 1, which are proteins of a hydrophobic nature, as in plasma they relate to lipoprotein particles. Thus, Triton X-114-based CPE is a simple plasma prefractionation tool, attractive for detailed 2DE studies of hydrophobic plasma proteins and their...

  11. Type 3 von Willebrand’s disease in a Shetland sheepdog

    OpenAIRE

    Pathak, Elizabeth J.

    2004-01-01

    A 3.75-year-old, spayed female, Shetland sheepdog was presented with excessive bleeding from a gingival surface. The dog’s condition deteriorated over 24 h and whole fresh blood was transfused. Type 3 von Willebrand’s disease was diagnosed. Coagulation was achieved and the dog was released with recommendations for a sedentary lifestyle and careful monitoring.

  12. Inflammatory reactions in placental blood of Plasmodium falciparum-infected women and high concentrations of soluble E-selectin and a circulating P. falciparum protein in the cord sera

    DEFF Research Database (Denmark)

    Jakobsen, P H; Rasheed, F N; Bulmer, J N; Theisen, M; Ridley, R G; Greenwood, B M

    1998-01-01

    significance. P. falciparum exoantigen, GLURP, was detected in cord blood indicating transplacental passage of malarial antigens. Concentrations of E-selectin were higher in cord blood samples compared with peripheral blood samples. This appeared to be associated with development of cord endothelial cells and...... falciparum protein, glutamate-rich protein (GLURP) and antibodies to P. falciparum rhoptry-associated protein-1 were measured among 105 Gambian women and their neonates. Peripheral blood concentrations of IL-10, soluble cytokine receptors and soluble adhesion molecules were found to be different from those...... immunoprivileged site. Concentrations of soluble vascular cell adhesion molecule-1 (sVCAM-1) and soluble intracellular adhesion molecule-1 (sICAM-1), potential adhesion receptors for malaria parasites, were associated with an active P. falciparum infection in the placenta although the associations did not reach...

  13. Interaction of colloidal gold nanoparticles with human blood: effects on particle size and analysis of plasma protein binding profiles

    OpenAIRE

    Dobrovolskaia, Marina A.; Patri, Anil K.; Zheng, Jiwen; Clogston, Jeffrey D.; Ayub, Nader; Aggarwal, Parag; Neun, Barry W.; Hall, Jennifer B.; McNeil, Scott E.

    2008-01-01

    Nanoparticle size and plasma binding profile contribute to a particle’s longevity in the bloodstream, which can have important consequences for therapeutic efficacy. In this study an approximate doubling in nanoparticle hydrodynamic size was observed upon in vitro incubation of 30- and 50-nm colloidal gold in human plasma. Plasma proteins that bind the surface of citrate-stabilized gold colloids have been identified. Effects of protein binding on the nanoparticle hydrodynamic size, elements o...

  14. Effects of Momordica charantia on osmotic fragility and label red blood cells and plasmatic protein with 99m-Tc in vitro

    Energy Technology Data Exchange (ETDEWEB)

    Magnata, Simey S.L.P. [Pernambuco Univ., Recife, PE (Brazil). Dept. de Energia Nuclear]. E-mail: sfmagnata@terra.com.br; Correia, Marilia B.L.; Brandao, Jose Odinilson C.; Souza, Grace M.L.; Catanho, Maria Teresa J.A. [Pernambuco Univ., Recife, PE (Brazil). Dept. de Biofisica e Radiobiologia; Terra, Daniele A.; Amorim, Lucia F. [Rio Grande do Norte Univ., Natal, RN (Brazil). Dept. de Fisiologia

    2005-07-01

    The use of natural products in the treatment physiopathology awaken the interest in the inquiry of the action mechanisms. The Momordica charantia, Melao de Sao Caetano, is used in the Caribbean and Orient for the diseases as stomatitis, cancer and diabetes. This work aims to verify the effect of the Momordica charantia's aqueous extract leaves on osmotic fragility and on labeling red blood cells (RBC) and plasmatic proteins with {sup 99m}Tc in vitro. To evaluate the osmotic fragility, samples of heparinized blood (500 mL) was incubed for 1 hour with brut extract (500 mL) in different concentrations (0; 10; 50 and 100% v/v); after centrifugation, the RCB were submitted the incubation (1 hour) with a gradient of NaCl (0;0,1;0,25;0,4;0,7 and 0.9%), the OD of supernatant was determined. With regards to label red blood cells and plasmatic proteins with {sup 99m}Tc in vitro was carried out by incubating of anticoagulant whole blood (500 mL) for 1 hour with brut extract (500 mL) in different concentrations (0; 10; 50 and 100% v/v). A stannous chloride solution of 1,2 {mu}g/mL was added the incubation for 60 minutes. After this the {sup 99m}Tc (3,7 MBq) was added and the incubation was continued for another 10 minutes. Those were centrifuged, precipitated with trichloroacetic acid 5% and mensured in a counter. The results shows that with regard to osmotic fragility, only the extract in the concentration of 100% provoked hemolysis. The Momordica charantia's extract is an agent who modify the fixation of {sup 99m}Tc in red blood cells. The results show with regard to osmotic fragility, only the extract in the quantity 100% provoked hemolysis. It is concluded that the Momordica charantia's extract is an agent who unchains the cellular fragility and {sup 99m}Tc fixation, showing a reduction effect. (author)

  15. Effects of Momordica charantia on osmotic fragility and label red blood cells and plasmatic protein with 99m-Tc in vitro

    International Nuclear Information System (INIS)

    The use of natural products in the treatment physiopathology awaken the interest in the inquiry of the action mechanisms. The Momordica charantia, Melao de Sao Caetano, is used in the Caribbean and Orient for the diseases as stomatitis, cancer and diabetes. This work aims to verify the effect of the Momordica charantia's aqueous extract leaves on osmotic fragility and on labeling red blood cells (RBC) and plasmatic proteins with 99mTc in vitro. To evaluate the osmotic fragility, samples of heparinized blood (500 mL) was incubed for 1 hour with brut extract (500 mL) in different concentrations (0; 10; 50 and 100% v/v); after centrifugation, the RCB were submitted the incubation (1 hour) with a gradient of NaCl (0;0,1;0,25;0,4;0,7 and 0.9%), the OD of supernatant was determined. With regards to label red blood cells and plasmatic proteins with 99mTc in vitro was carried out by incubating of anticoagulant whole blood (500 mL) for 1 hour with brut extract (500 mL) in different concentrations (0; 10; 50 and 100% v/v). A stannous chloride solution of 1,2 μg/mL was added the incubation for 60 minutes. After this the 99mTc (3,7 MBq) was added and the incubation was continued for another 10 minutes. Those were centrifuged, precipitated with trichloroacetic acid 5% and mensured in a counter. The results shows that with regard to osmotic fragility, only the extract in the concentration of 100% provoked hemolysis. The Momordica charantia's extract is an agent who modify the fixation of 99mTc in red blood cells. The results show with regard to osmotic fragility, only the extract in the quantity 100% provoked hemolysis. It is concluded that the Momordica charantia's extract is an agent who unchains the cellular fragility and 99mTc fixation, showing a reduction effect. (author)

  16. The Effect of Supplementation Urea and Sulfur in Mixed Cassava Waste Fermented and Soyabean Cake Waste on Digestibility of Protein and Blood Urea Male Sheep

    Directory of Open Access Journals (Sweden)

    M Bata

    1999-05-01

    Full Text Available Eighteen local male sheep were divided into 3 groups I,II and III based on the body weight 18.55±1.27, 15.79±0.67 and 13.14±1.33 kg respectively. Two level urea (2 and 3% and three levels Sulfur (0.02 and 0.3% as treatment, so pattern factorial 2x3 with Randomized Block Design used this experiment. All of the treatment get a same basal feed namely land-grass and concentrate with dry matter ratio 70:30. The total intake of dry matter was 4 % of body weight. The concentrate consist of cassava waste fermented and soyabean cake waste with dry matter ratio 77.50 : 22.50. Supplementation of urea and sulfur shown not significant interaction, but supplementation urea had effect high significantly (P<0.01 on digestibility of protein and sulfur only had effect significant (P<0.05 on blood urea. These result had indication that enriched urea in the diet could increase protein digestibility and sulfur level 0.2% could prevent NH3 absorption from rumen. (Animal Production 1(2: 75-81 (1999Key Words: cassava waste, soyabean cake waste, fermentation, digestibility, urea blood.

  17. Comparison of coagulation factors and blood loss between O and non-O blood types following hydroxyethyl starch infusion

    OpenAIRE

    Choi, Soo Joo; Ahn, Hyun Joo; Lee, Jae Ik

    2010-01-01

    Background Individuals with type O blood are more likely to have reduced factor VIII and von Willebrand factor levels compared to their non-O counterparts. Hydroxyethyl starch (HES), which is widely used for blood volume replacement, can induce coagulopathy. Therefore, we tested whether blood type O patients show more coagulopathy and blood loss than non-O patients after infusion of 6% HES. Methods Thirty-four non-O and 20 type O patients scheduled for posterior lumbar interbody fusion (PLIF)...

  18. Laboratory tests used to help diagnose von Willebrand disease: an update.

    Science.gov (United States)

    Favaloro, Emmanuel J; Pasalic, Leonardo; Curnow, Jennifer

    2016-06-01

    von Willebrand disease (VWD) is due to quantitative deficiencies and/or qualitative defects in von Willebrand factor (VWF), and is reportedly the most common inherited bleeding disorder. However, diagnosis of VWD is problematic, and is subject to over-, under-, and misdiagnosis. This is due to many factors, including limitations in current test procedures and an over-reliance on these imperfect test systems for clinical diagnosis. VWF is a complex plasma protein with multiple functions, but essentially acts to assist in the formation of a platelet thrombus to stop blood loss from sites of injury. VWF achieves this by several activities, including binding to platelets [primarily through the glycoprotein Ib (GPIb) receptor], binding to subendothelial matrix components (primarily collagen), and binding to factor VIII (FVIII), thus protecting FVIII from degradation and enabling its delivery to sites of vascular injury. Laboratory assessment of VWD entails performance of a battery of tests, some of which aim to mimic in vivo VWF activity. VWD is classified into six separate types, based on quantitative deficiencies [types 1 (partial deficiency) and 3 (total deficiency)] of VWF, or qualitative defects (type 2 VWD), which comprise four 'subtypes'. The current report briefly overviews the diagnosis of VWD, describing the currently available armamentarium of laboratory tests, as well as emerging options for laboratory-assisted diagnostics. Although some methodologies suffer from significant limitations that challenge the accurate diagnosis of VWD, newer methodologies and specific approaches can improve detection of this common bleeding disorder, and the appropriate characterisation and typing of patients. PMID:27131932

  19. Comparison of human memory CD8 T cell responses to adenoviral early and late proteins in peripheral blood and lymphoid tissue.

    Directory of Open Access Journals (Sweden)

    Amita Joshi

    Full Text Available Treatment of invasive adenovirus (Ad disease in hematopoietic stem cell transplant (SCT recipients with capsid protein hexon-specific donor T cells is under investigation. We propose that cytotoxic T cells (CTLs targeted to the late protein hexon may be inefficient in vivo because the early Ad protein E3-19K downregulates HLA class I antigens in infected cells. In this study, CD8+ T cells targeted to highly conserved HLA A2-restricted epitopes from the early regulatory protein DNA polymerase (P-977 and late protein hexon (H-892 were compared in peripheral blood (PB and tonsils of naturally infected adults. In tonsils, epitope-specific pentamers detected a significantly higher frequency of P-977+CD8+ T cells compared to H-892+CD8+ T cells; this trend was reversed in PB. Tonsil epitope-specific CD8+ T cells expressed IFN-γ and IL-2 but not perforin or TNF-α, whereas PB T cells were positive for IFN-γ, TNF-α, and perforin. Tonsil epitope-specific T cells expressed lymphoid homing marker CCR7 and exhibited lower levels of the activation marker CD25 but higher proliferative potential than PB T cells. Finally, in parallel with the kinetics of mRNA expression, P-977-specific CTLs lysed targets as early as 8 hrs post infection. In contrast, H-892-specific CTLs did not kill unless infected fibroblasts were pretreated with IFN-γ to up regulate HLA class I antigens, and cytotoxicity was delayed until 16-24 hours. These data show that, in contrast to hexon CTLs, central memory type DNA polymerase CTLs dominate the lymphoid compartment and kill fibroblasts earlier after infection without requiring exogenous IFN-γ. Thus, use of CTLs targeted to both early and late Ad proteins may improve the efficacy of immunotherapy for life-threatening Ad disease in SCT recipients.

  20. Extracorporeal irradiation of calves blood. Effects on: the lymphocytes, the blood-platelet function, seric proteins, and fibrinogen; Irradiation extracorporelle du sang de veau effets sur: les lymphocytes, la fonction plaquettaire, les proteines seriques et le fibrinogene

    Energy Technology Data Exchange (ETDEWEB)

    Hollard, D.; Suscillon, M.; Benabid, Y.; Concord, E.; Ivanoff, M.; Laurent, M.; Rambaud, F. [Commissariat a l' Energie Atomique, Grenoble (France). Centre d' Etudes Nucleaires

    1969-07-01

    The present paper reports the results obtained after extracorporeal irradiation of circulating blood of calves. Animals are divided in 3 groups as follows: - control animals: blood circulation without irradiation; - calves which received 40000 rads during 24 hours of continuous irradiation; - calves which received the same dose, during a period of 5 days (5 hours every day). The more interesting results are: - the early lymphopenia which persists for 7 or 8 weeks and may be in relationship with the change of immunoglobulins; - a constant hyperfibrinemia (12 g/l) never reported, as far as we know, by authors using I.E.C. Several hypothesis are advanced to explain this phenomenon. (authors) [French] Ce travail presente l'ensemble des resultats hematologiques obtenus apres irradiation extracorporelle du sang de veaux repartis en 3 series: - veaux temoins: circulation du sang sans irradiation - veaux soumis a une irradiation continue dose globale integree de 40000 rads en 24 heures; - veaux ayant recu la meme dose globale en irradiation fractionnee repartie sur 5 jours (5 heures par jour). Les resultats les plus marquants sont d'une part une lymphopenie precoce se prolongeant durant 7 a 8 semaines et qui pourrait etre reliee aux modifications observees sur les immunoglobulines. D'autre part une hyperfibrinemie (12 g/l) constante, apparemment jamais signalee par d'autres auteurs utilisant l'I.E.C. Plusieurs hypotheses explicatives de ce phenomene sont exposees et sont a l'etude. (auteurs)

  1. Effects of prebiotic (Fermacto in low protein diet on some blood parameters and intestinal microbiota of broiler chicks

    Directory of Open Access Journals (Sweden)

    Amir Attar

    2010-01-01

    Full Text Available This study was conducted to evaluate the effects of prebiotic (Fermacto in low protein diet on serum cholesterol and intestinal microbiota of broiler chicks. One hundred and fifty six 1-day old Ross 308 broiler chicks of both sexes were used for 42 days. The chicks were randomly allocated to 12 pens containing 13 chicks each with 3 replicates and assigned to receive one of the 4 dietary treatments of 2 levels of protein (low and high and 2 levels of prebiotic (0 and 0.2% in a completely randomised design with factorial arrangement. There were no significant differences in serum HDL and LDL levels among treatments. Significant differences were observed in serum cholesterol and intestinal microflora between the high protein diet without prebiotic and the low protein diet containing prebiotic (P<0.05. The results of the present experiment showed that the addition of prebiotic to broiler diets containing 90% of the NRC protein recommendation significantly affects serum cholesterol and intestinal microflora of broiler chicks (P<0.05.

  2. Der Einfluss von Relaxin auf das Wachstum von Mammakarzinomen

    OpenAIRE

    Habla, Christiane

    2010-01-01

    Brustkrebs ist die häufigste Krebstodesursache bei Frauen in den Industrienationen mit einer jährlich ansteigenden Neuerkrankungsrate (Senn und Niederberger 2002). Durch vorangegangene Untersuchungen wurde bereits deutlich, dass das Peptidhormon Relaxin unter in vitro Bedingungen maßgeblich zur Tumorprogression von Mammakarzinomen beiträgt (Binder et al. 2002). Die vorliegende Arbeit hat untersucht, ob Relaxin diese Wirkung auch in vivo auf Mammakarzinome ausübt. Relaxin ist...

  3. Entwicklung von Analyseverfahren zur Bestimmung von Ochratoxin A in Lebensmitteln

    OpenAIRE

    Reinsch, Martin

    2006-01-01

    Mykotoxine sind giftige Naturstoffe, die im Rahmen des Sekundärstoffwechsels von Schimmelpilzen beim Wachstum auf pflanzlichen Substraten gebildet werden. Zu den bekanntesten Mykotoxinen zählt das Ochratoxin A (OTA), welches überwiegend in Getreide und davon abgeleiteten Erzeugnissen, aber auch in Wein und Kaffee sowie Gewürzen und Bier nachgewiesen wurde. OTA ist unter anderem immunotoxisch, nephrotoxisch und besitzt teratogene sowie kanzerogene Eigenschaften. Darüber hinaus wird OTA eine ...

  4. Diagnostik von Niederdruckplasmen zur kontrollierten Synthese von Kohlenwasserstoff-Nanoteilchen

    OpenAIRE

    Schulze, Martin

    2007-01-01

    Die vorliegende Dissertation untersucht das Verhalten einer Niederdruckentladung, die zur Erzeugung von Kohlenwasserstoff-Nanoteilchen verwendet wird. Plasmasynthetisierte Nanoteilchen haben eine enge Größenverteilung wie sie für neue Anwendungen in der Elektronik benötigt wird. Ein ungelöstes Problem ist allerdings die Kontrolle der Größenverteilung während des Wachstumsprozesses. Kern der vorliegenden Arbeit ist die Untersuchung einer Plasmainstabilität, die in einem induktiven ...

  5. Sekretomanalyse von Pleurotus sapidus zum effizienten Aufschluss von Lignocellulosen

    OpenAIRE

    Schüttmann, Ina

    2011-01-01

    Mit einer polyvalenten Peroxidase wurde ein Schlüsselenzym des Lignocelluloseabbaus aus Kulturen des Basidiomyceten Pleurotus sapidus chromatographisch gereinigt. Nach einer dreistufigen Reinigung wurde das Enzym mit einem Reinigungsfaktor von 130 aus dem Überstand einer Submerskultur isoliert. Die Aktivitätsoptima wurden für die Substrate b,b-Carotin, ABTS, Syringol und Veratrylalkohol ebenso bestimmt wie die Wechselzahlen und katalytischen Konstanten. In Zusammenarbeit mit der Firma Arte...

  6. Korrelation von Biometrie und Genom von Mäuseeizellen

    OpenAIRE

    Petersen, Katrin

    2010-01-01

    Die vorliegende Arbeit beschäftigt sich mit der Farge, ob eine Korrelation von Biometrie und Genom bei Mäuseeizellen besteht. Dadurch soll versucht werden, die Schwangerschaftsrate im Rahmen assistierter Fertilisation durch optische Beurteilung der Eizellen und Vorauswahl anhand morphologischer und biometrischer Kriterien, also nicht invasiver Maßmahmen, zu erhöhen. Die Untersuchungen erfolgten an Mäuseeizellen und sollen eine mögliche Übertragbarkeit auf menschliche Eizellen herstellen mit W...

  7. SLM von Polylactiden zur Herstellung von resorbierbaren Implantaten

    OpenAIRE

    Höges, Simon; Meiners, Wilhelm

    2009-01-01

    Rapid Manufacturing von individuellen Implantaten mittels Selective Laser Melting (SLM) wurde für metallische Standardwerkstoffe in der Medizintechnik (Titanlegierungen, Kobalt-Chrom-Legierungen, Stahl) realisiert. Dies sind permanente Implantate, die entweder dauerhaft im Körper verbleiben oder in einer zweiten Operation entfernt werden. Eine vom ILT mittels SLM hergestellte Hüftpfanne aus TiAl6V4 konnte bereits erfolgreich im Patienten implantiert werden. Die regenerative Therapie ist jedoc...

  8. Effects of prebiotic (Fermacto) in low protein diet on some blood parameters and intestinal microbiota of broiler chicks

    OpenAIRE

    Amir Attar; Farid Firouzbakhsh; Hadi Sayyahzadeh; Mansour Rezaei; Mahalam Ghiyasi

    2010-01-01

    This study was conducted to evaluate the effects of prebiotic (Fermacto) in low protein diet on serum cholesterol and intestinal microbiota of broiler chicks. One hundred and fifty six 1-day old Ross 308 broiler chicks of both sexes were used for 42 days. The chicks were randomly allocated to 12 pens containing 13 chicks each with 3 replicates and assigned to receive one of the 4 dietary treatments of 2 levels of protein (low and high) and 2 levels of prebiotic (0 and 0.2%) in a completely ra...

  9. Synergistische Aktivierung von dendritischen Zellen durch Kombination von TLR Liganden und Untersuchung von Dextranbasierenden Nanopartikeln als Carrier in der Immuntherapie

    OpenAIRE

    Krummen, M. (Mathias)

    2007-01-01

    Die adäquate Aktivierung von dendritischen Zellen (DC) stellt eine Voraussetzung für den Einsatz dieser Zellen in der antitumoralen Immuntherapie dar. Toll-like Rezeptor (TLR) Liganden haben sich als potente Aktivatoren erwiesen und zeigen in Kombination teilweise synergistische Effekte. In der vorliegenden Arbeit wurde die synergistische Aktivierung von DC durch Kombination verschiedener TLR Liganden anhand der Freisetzung von IL-12 und der TH1-polarisierenden Wirkung der Zellen untersucht. ...

  10. Identifizierung von fünf Tegumentproteinen (UL37, UL46, UL47, UL48, UL49) sowie von zwei Nichtstrukturproteinen (UL31, gG) des Virus der infektiösen Laryngotracheitis der Hühner und Evaluierung einer UL47-Deletionsmutante als potentielle Lebendvirus-Vakzine

    OpenAIRE

    Wiesner, Dorothee

    2006-01-01

    In der vorliegenden Arbeit wurden die ILTV-Homologen von sieben konservierten alphaherpesviralen Genen untersucht. Durch Northern Blot-Analysen wurden die viralen mRNAs identifiziert und deren Expressionskinetik in infizierten Zellen bestimmt. Mit Hilfe monospezifischer Antiseren, die nach Immunisierung von Kaninchen mit bakteriellen Fusionsproteinen erhalten wurden, konnten die entsprechenden viralen Proteine nachgewiesen werden. Diese zeigten in Western Blot-Analysen apparente Massen von 33...

  11. Funktionsaufklärung von Atmungsproteinen in Insekten

    OpenAIRE

    Gleixner, Eva Maria

    2011-01-01

    Globine sind kleine globuläre Proteine mit nahezu ubiquitärem Vorkommen in allen Tiergruppen. Sie weisen eine typische Sandwichstruktur auf, die in der Regel aus acht α-Helices mit einer zentralen prosthetischen Häm-Gruppe besteht und die Proteine zur Bindung gasförmiger Liganden befähigt. Die Funktionen der Globine reichen von O2-Transport und – Speicherung, über eine Beteiligung bei der Entgiftung reaktiver Sauerstoff- und Stickstoffspezies bis hin zu sensorischen physiologischen Aufgaben. ...

  12. Didaktische Konzeption von Serious Games: Zur Verknüpfung von Spiel- und Lernangeboten

    Directory of Open Access Journals (Sweden)

    Michael Kerres

    2009-08-01

    Full Text Available Serious Games versuchen Spielen mit Lernen zu verbinden. Der Beitrag problematisiert die Machbarkeit einer solchen Verknüpfung in digitalen Welten und diskutiert verschiedene didaktische Konzepte einer Nutzbarmachung digitaler Spiele für das Lernen und Lehren. Ausgehend von einer Analyse des Erlernens von Spielen werden drei didaktische Konzepte von Serious Games im Hinblick auf ihre didaktischen Potenziale untersucht: (1 der unmittelbare Transfer von Wissen, das im Spiel erworben wird (keine zusätzliche Didaktisierung, (2 Didaktisierung durch Einbettung des Spiels in einer Lernsituation oder (3 Didaktisierung durch Einbettung von Lernaufgaben in ein Spiel. Ergebnisse zur Nutzung von Spielen, bei denen Lernaufgaben in eine Spielwelt eingebettet sind, zeigen, dass Personen die Aufnahme von Wissen in einem expliziten Lernmodus eher zu reduzieren versuchen, um möglichst zügig wieder in den Spielmodus zu wechseln. Diskutiert werden die Konsequenzen dieser Problematik für die didaktische Konzeption von Serious Games.

  13. Blood pressure

    Science.gov (United States)

    ... the walls of the arteries is called blood pressure. Blood pressure is measured both as the heart contracts, which ... as it relaxes, which is called diastole. Normal blood pressure is considered to be a systolic blood pressure ...

  14. Blood transfusions

    Science.gov (United States)

    ... this page: //medlineplus.gov/ency/patientinstructions/000431.htm Blood transfusions To use the sharing features on this ... several sources of blood which are described below. Blood From the Public (Volunteer Blood Donation) The most ...

  15. Blood Basics

    Science.gov (United States)

    ... Patient Group Links Advocacy Toolkit Home For Patients Blood Basics Blood is a specialized body fluid. It ... about 9 pints. Jump To: The Components of Blood and Their Importance Many people have undergone blood ...

  16. Blood Thinners

    Science.gov (United States)

    If you have some kinds of heart or blood vessel disease, or if you have poor blood flow to your brain, your doctor may recommend that you take a blood thinner. Blood thinners reduce the risk of heart ...

  17. Blood culture

    Science.gov (United States)

    Culture - blood ... A blood sample is needed . The site where blood will be drawn is first cleaned with an antiseptic such ... organism from the skin getting into (contaminating) the blood sample and causing a false-positive result (see ...

  18. Detection of two amino acid deletions in HIV-1 Nef protein from Chinese former paid blood donors

    Institute of Scientific and Technical Information of China (English)

    SONG Yan-hui; WEI Min; MA Peng-fei; XING Hui; SI Xue-feng; TANG Hai-li; LI Hui-guang; SHAO Yi-ming

    2007-01-01

    @@ HIV-1 Nef protein plays an important role in HIV-1 pathogenesis, including downregulation of the cell surface CD4 and class Ⅰ major histocompatibility complex (MHC), enhancement of virion infectivity and alteration of a variety of cellular signal transduction pathways.

  19. Effect of dietary protein levels on growth performance, mortality rate and clinical blood parameters in mink (Mustela vison)

    DEFF Research Database (Denmark)

    Damgaard, B.M.; Clausen, T.N.; Dietz, Hans Henrik

    1998-01-01

    Effects of dietary protein levels ranging from 35% to 15% of metabolizable energy (ME) and dietary fat levels ranging in a reciprocal fashion from 47% to 67% of ME, and a constant dietary carbohydrate level of 18% of ME were investigated in male mink kits in the growing-furring period. Growth per...

  20. Aggressive periodontitis and chronic arthritis: blood mononuclear cell gene expression and plasma protein levels of cytokines and cytokine inhibitors

    DEFF Research Database (Denmark)

    Sørensen, Lars K; Havemose-Poulsen, Anne; Bendtzen, Klaus;

    2009-01-01

    BACKGROUND: Cytokines and cytokine inhibitors have been associated with many immunoinflammatory diseases. In the present study, we examined whether peripheral blood mononuclear cell (PBMC) gene expression mirrors the corresponding plasma levels of clinically important pro- and anti-inflammatory c......BACKGROUND: Cytokines and cytokine inhibitors have been associated with many immunoinflammatory diseases. In the present study, we examined whether peripheral blood mononuclear cell (PBMC) gene expression mirrors the corresponding plasma levels of clinically important pro- and anti.......001) or JIA (P = 0.002), and PBMC TNFA transcript levels were lower in patients with JIA (P = 0.001). A negative correlation was found between IL6 expression and IL-6 plasma levels in patients with JIA versus controls, and a positive correlation/association was found between TNFRI expression and s......TNF-RI plasma levels in patients with LAgP and RA. CONCLUSIONS: The study demonstrated only a few changes in the PBMC expression of various cytokine and cytokine inhibitor genes in aggressive periodontitis and chronic arthritis compared to controls. There were a few similarities among disease groups, and no...

  1. A combined blood based gene expression and plasma protein abundance signature for diagnosis of epithelial ovarian cancer - a study of the OVCAD consortium

    International Nuclear Information System (INIS)

    The immune system is a key player in fighting cancer. Thus, we sought to identify a molecular ‘immune response signature’ indicating the presence of epithelial ovarian cancer (EOC) and to combine this with a serum protein biomarker panel to increase the specificity and sensitivity for earlier detection of EOC. Comparing the expression of 32,000 genes in a leukocytes fraction from 44 EOC patients and 19 controls, three uncorrelated shrunken centroid models were selected, comprised of 7, 14, and 6 genes. A second selection step using RT-qPCR data and significance analysis of microarrays yielded 13 genes (AP2A1, B4GALT1, C1orf63, CCR2, CFP, DIS3, NEAT1, NOXA1, OSM, PAPOLG, PRIC285, ZNF419, and BC037918) which were finally used in 343 samples (90 healthy, six cystadenoma, eight low malignant potential tumor, 19 FIGO I/II, and 220 FIGO III/IV EOC patients). Using new 65 controls and 224 EOC patients (thereof 14 FIGO I/II) the abundances of six plasma proteins (MIF, prolactin, CA125, leptin, osteopondin, and IGF2) was determined and used in combination with the expression values from the 13 genes for diagnosis of EOC. Combined diagnostic models using either each five gene expression and plasma protein abundance values or 13 gene expression and six plasma protein abundance values can discriminate controls from patients with EOC with Receiver Operator Characteristics Area Under the Curve values of 0.998 and bootstrap .632+ validated classification errors of 3.1% and 2.8%, respectively. The sensitivities were 97.8% and 95.6%, respectively, at a set specificity of 99.6%. The combination of gene expression and plasma protein based blood derived biomarkers in one diagnostic model increases the sensitivity and the specificity significantly. Such a diagnostic test may allow earlier diagnosis of epithelial ovarian cancer

  2. Investigations on early reactions of lymphocyte proteins on gamma irradiation of human blood and their dose dependence. Preconditions for the development of an individual radiobiological dosimeter

    International Nuclear Information System (INIS)

    The present thesis is concerned with the issues involved in obtaining reliable experimental data permitting a retrospective assessment of radiation-induced doses at the time of application or contamination. In order to provide prompt medical treatment of those injured in accidents with ionizing radiation, biological procedures that can be implemented swiftly and at an early stage are required both to determine the radiation dose originally received as well as to assess the course of the dosedependent biological reactions on the basis of individual sensitivity to radiation. To this end, in the present thesis the lymphocyte proteins (phosphoproteins and total proteins) in blood taken from test subjects who had been exposed to γ-radiation (applied dose: 0-4 Gy) were analysed just 15 minutes after completing irradiation by means of 2D gel electrophoresis. Only those early-response proteins (ERPROs) that displayed a significant radiation-induced change were identified by nano-HPLC-MS/MS. For validation purposes, the dose-dependent gene expression of some of these proteins was determined by RT-qPCR. The following ERPROs displayed pronounced early reactions in the form of changes of concentration in comparison to unirradiated control samples: talin-1, talin-2, β-actin, mutant β-actin, peroxin-1 and also the phosphoproteins annexin-A6, MHCbinding protein-2, zyxin-2, interleukin-17E and phosphoglycerate kinase-1. The majority of the lymphocyte ERPROs represent proteins responsible for changes to the cytoskeleton, proliferation and cell cycle, modulation of immunoreactions as well as protein degradation and energy production. Other cellular processes may not have been determined due to the sensitivity restrictions of the 2DPAGE and MS methods, but cannot be excluded. Gene expression studies revealed that a combination of methods, comprising RT-qPCR and 2D-PAGE as well as DNA microarray and Western blot, may in future be able to overcome these restrictions. The slopes of

  3. Entwicklung von Methoden zur automatischen Generierung, grafischen Darstellung und interaktiven Analyse von metabolischen Netzwerken

    OpenAIRE

    Schunk, Ralph Oliver

    2006-01-01

    Die große Anzahl der metabolischen Reaktionen und der an ihnen beteiligten Komponenten kann nur mit Methoden zur automatisierten Darstellung und Analyse von metabolischen Netzwerken erforscht werden. Mit dem CUBIC Pathway Editor "Cupe" existiert jetzt ein Programm, das auf einzigartige Art und Weise die Generierung, Darstellung und Analyse von Stoffwechselnetzwerken mit den aktuellsten Methoden zum automatischen Zeichnen von Graphen verbindet. Dank eines integrierten Datenbestandes von ca. 32...

  4. Der Thiol:Disulfid-Redox Metabolismus und der Blaulichtrezeptor Lmo0799 von Listeria monocytogenes

    OpenAIRE

    Ondrusch, Nicolai

    2010-01-01

    Der Thiol-Redox-Metabolismus, der in allen lebenden Zellen zu finden ist, wirkt oxidativem Stress entgegen. Des Weiteren dient er auch der Aufrechterhaltung der intrazellulären Thiol:Disulfid-Balance, die wiederum für die Funktion vieler Proteine essentiell ist. Auch stellt er Reduktionsäquivalente für die Produktion von Desoxyribonucleotiden für die DNA-Synthese bereit und hilft oxidierte Proteine zu reparieren. Der Thiol:Disulfid-Redox-Metabolismus (TDRM) unterscheidet sich von anderen meta...

  5. Evaluierungsbericht Systems Engineer von LBMS

    OpenAIRE

    Herzwurm, Georg

    1994-01-01

    Hersteller von Systems Engineer ist die 1977 gegründete Firma Learmonth and Burchen Management Systems Pie. (LBMS) mit Sitz in London. Systems Engineer ist eine Client-Server-Lösung unter DOS/Windows, die standardmäßig aus den Komponenten Systems Engineer (Analyse und Design, Projektadministration), SE/Repository (Konfigurations management, Entwicklungsdatenmanagement) und SE/Workplace (Dokumentation) besteht. Als jeweils separat zu erwerbende Komponenten werden darüber hinaus Process Enginee...

  6. Scipios Realisation von Luciers Raumkomposition

    OpenAIRE

    Presas, Miguel A.

    2011-01-01

    In diesem Vortrag werden vier verschiedene Realisationen von Alvin Luciers Raumkomposition "I Am Sitting in a Room" (1970) verglichen. Auch der Komponist Agostino di Scipio hat sich intensiv mit dem Stück auseinander gesetzt, sowohl als Interpret als auch als Musiktheoretiker. Ihn interessiert vor allem die zentrale Rolle des Raumes und der Technologie bei einer klanglichen Realisation des Stücks. Der Raum wirkt als akustischer Filter. In Alvin Luciers I Am Sitting in a Room wird die Aufnahme...

  7. Elementspeziation von Neptunium im Ultraspurenbereich

    OpenAIRE

    Stöbener, Nils

    2013-01-01

    In der vorliegenden Arbeit wurde der Nachweis des Isotops Np-237 mit Resonanzionisations-Massenspektrometrie (RIMS) entwickelt und optimiert. Bei RIMS werden Probenatome mehrstufig-resonant mit Laserstrahlung angeregt, ionisiert und anschließend massenspektrometrisch nachgewiesen. Die Bestimmung geeigneter Energiezustände für die Anregung und Ionisation von Np-237 erfolgte durch Resonanzionisationsspektroskopie (RIS), wobei über 300 bisher unbekannte Energieniveaus und autoionisierende Zustän...

  8. Information als Gegenstand von Informationskompetenz

    OpenAIRE

    Ingold, Marianne

    2011-01-01

    Der Informationsbegriff als zentraler Gegenstand von Informationskompetenz wird in der bibliothekarischen Diskussion in der Regel nicht explizit thematisiert, sondern lässt sich aus Fachliteratur und Bibliothekspraxis nur implizit erschließen. Eine theoretische Beschäftigung mit dem Informationsbegriff ist jedoch unabdingbar, soll das Konzept "Informationskompetenz" auch außerhalb des bibliothekarischen Kontextes verständlich und nutzbar gemacht werden. Im vorliegenden Text, der sich als Beit...

  9. Bestimmung von Tocopheroloxidationsprodukten im Humanserum

    OpenAIRE

    Pollok, Dagmar

    2004-01-01

    Die vorliegende Arbeit beschäftigt sich mit den Tocopherolen (Vitamin E) und ihren Oxidationsprodukten, wobei der Schwerpunkt bei der Entwicklung einer Methode zur Bestimmung von Tocopheroloxidationsprodukten in Humanserumproben lag. Die Methodenentwicklung konzentrierte sich auf die Bestimmung des alpha-Tocopherolchinons (alpha-TQ) als bedeutendes Oxidationsprodukt des wirksamsten und im Serum dominierenden Vertreters der Vitamin-E-Gruppe, des alpha-Tocopherols. Weiterhin wurde die Möglichke...

  10. Penetrationseigenschaften von beschichtetem mikrofeinem Titandioxid

    OpenAIRE

    Rickmeyer, Christiane

    2002-01-01

    Zielsetzung dieser Arbeit war es, das Verhalten der in modernen Sonnenschutzmitteln eingesetzten Titandioxid-Mikropartikel mit quantitativen Methoden zu bestimmen, um Aussagen über ihre Eignung zu erhalten. Dabei stand die Frage nach der Verteilung der Substanz innerhalb des Stratum corneum im Mittelpunkt der Untersuchungen. Insbesondere war wegen der bekannten photokatalytischen Aktivität von Titandioxid zu klären, ob ein Kontakt mit den lebenden Bereichen der Haut ausgeschlossen werden kann...

  11. Auswirkungen von Langlaufloipen auf Moorvegetation

    OpenAIRE

    Lanvers, Jan; Sieg, Birgit; Fartmann, Thomas

    2014-01-01

    Moore unterliegen auf Grund ihrer zahlreichen spezialisierten Arten und zugleich starken Gefährdung dem Schutz der EU Fauna-Flora-Habitat-Richtlinie. In Süddeutschland, am Rand der Alpen, ist noch eine Vielzahl naturschutzfachlich wertvoller Moore vorhanden. Teilweise verlaufen durch diese Moore Langlaufloipen, über deren Wirkung auf die Vegetation bislang allerdings nur wenig bekannt ist. In dieser Arbeit werden deshalb die Auswirkungen von Langlaufloipen auf Standort, Flora und Vegetation v...

  12. Prognose von Gewerbemieten in Deutschland

    OpenAIRE

    Ludwig, Helge

    2007-01-01

    Die Arbeit untersucht die Prognose von Gewerbemieten in Deutschland und entwickelt geeignete Mietprognosemodelle. Hierzu werden Marktforschungsmethoden auf die Immobilienökonomie übertragen. Die disperse Struktur des deutschen Immobilienmarktes erfordert die Entwicklung unterschiedlicher Prognosemodelle unter Berücksichtigung der jeweiligen Marktbesonderheiten. Einzelne Marktsegmente werden anhand der Nutzungsart und der räumlichen Abgrenzung definiert. Für diese Teilmärkte liegen jeweils Mar...

  13. Combining viral vectored and protein-in-adjuvant vaccines against the blood-stage malaria antigen AMA1: report on a phase 1a clinical trial.

    Science.gov (United States)

    Hodgson, Susanne H; Choudhary, Prateek; Elias, Sean C; Milne, Kathryn H; Rampling, Thomas W; Biswas, Sumi; Poulton, Ian D; Miura, Kazutoyo; Douglas, Alexander D; Alanine, Daniel Gw; Illingworth, Joseph J; de Cassan, Simone C; Zhu, Daming; Nicosia, Alfredo; Long, Carole A; Moyle, Sarah; Berrie, Eleanor; Lawrie, Alison M; Wu, Yimin; Ellis, Ruth D; Hill, Adrian V S; Draper, Simon J

    2014-12-01

    The development of effective vaccines against difficult disease targets will require the identification of new subunit vaccination strategies that can induce and maintain effective immune responses in humans. Here we report on a phase 1a clinical trial using the AMA1 antigen from the blood-stage Plasmodium falciparum malaria parasite delivered either as recombinant protein formulated with Alhydrogel adjuvant with and without CPG 7909, or using recombinant vectored vaccines--chimpanzee adenovirus ChAd63 and the orthopoxvirus MVA. A variety of promising "mixed-modality" regimens were tested. All volunteers were primed with ChAd63, and then subsequently boosted with MVA and/or protein-in-adjuvant using either an 8- or 16-week prime-boost interval. We report on the safety of these regimens, as well as the T cell, B cell, and serum antibody responses. Notably, IgG antibody responses primed by ChAd63 were comparably boosted by AMA1 protein vaccine, irrespective of whether CPG 7909 was included in the Alhydrogel adjuvant. The ability to improve the potency of a relatively weak aluminium-based adjuvant in humans, by previously priming with an adenoviral vaccine vector encoding the same antigen, thus offers a novel vaccination strategy for difficult or neglected disease targets when access to more potent adjuvants is not possible. PMID:25156127

  14. Activity of the Respiratory Chain Enzymes of Blood Leucocytes’ Mitochondria Under the Conditions of Toxic Hepatitis Induced Against the Background Alimentary Deprivation of Protein

    Directory of Open Access Journals (Sweden)

    O.N. Voloshchuk

    2015-12-01

    Full Text Available Full functioning of the leucocytes’ energy supply system is one of the essential factors for the immune surveillance system effective work. The pivotal enzymes of the leucocytes’ energy biotransformation system are NADH-ubiquitin reductase, a marker of the Complex I of respiratory chain activity, and succinate dehydrogenase, key enzyme of the Complex II of respiratory chain. The aim of research – to study the NADH-ubiquitin reductase and succinate dehydrogenase activity of the blood leucocytes’ mitochondria under the conditions of toxic hepatitis induced against the background alimentary deprivation of protein. It is shown, that under the conditions of acetaminophen-induced hepatitis a reduction of the NADH-ubiquitin reductase enzymatic activity is observed on the background activation of the succinate-dependent way of the mitochondrial oxidation. Conclusion was made that alimentary deprivation or protein is a factor, aggravating the misbalance of the energy biotransformation system in the leucocytes of rats with toxic hepatitis. Established activity changes of the leucocytes’ mitochondria respiratory chain key enzymes may be considered as one of the mechanisms, directed on the maintenance of leucocytes energy supply on a level, sufficient for their functioning. Research results may be used for the biochemical rationale of the therapeutic approaches to the elimination and correction of the leucocytes’ energy metabolism disturbances consequences under the conditions of acetaminophen-induced hepatitis, aggravated by the alimentary protein deprivation.

  15. Dr. von Braun Briefing Walt Disney

    Science.gov (United States)

    1965-01-01

    Dr. von Braun began his association with Walt Disney in the 1950s when the rocket scientist appeared in three Disney television productions related to the exploration of space. Years later, Dr. von Braun invited Disney and his associates to tour the Marshall Space Flight Center (MSFC) in Huntsville, Alabama. This photograph is dated April 13, 1965. From left are R.J. Schwinghamer from the MSFC, Disney, B.J. Bernight, and Dr. von Braun.

  16. Offenes Baukastensystem zur effizienten Dimensionierung von Materialflusssystemen

    OpenAIRE

    Meinhardt, Ingolf; Marquardt, Hans-Georg

    2007-01-01

    Die optimale Gestaltung logistischer Systeme und Prozesse bekommt eine immer größere Bedeutung für die Wirtschaftlichkeit und Wettbewerbsfähigkeit von Unternehmen. Für Einzelkomponenten von Materi-alflusssystemen sind neben exakten analytischen Verfahren auch Näherungslösungen und Ersatzmodelle in Form von Polynomen, neuronalen Netzen oder zeitdiskreten Verfahren vorhanden, mit denen eine gute Nachbildung des Verhaltens dieser Komponenten möglich ist. Ziel des Baukastensystems ist es, für...

  17. Nichtlineare Rauschmodellierung von LC Tank VCOs

    OpenAIRE

    T. Thiessen; Bremer, J.-K.; Mathis, W.

    2008-01-01

    Im Rahmen dieser Arbeit wird ein alternativer Ansatz zur Phasenrauschoptimierung von LC-Tank Oszillatoren (VCOs) unter Verwendung stochastischer Differentialgleichungen vorgestellt. Zunächst werden die linearen Ansätze von Leeson, Hajimiri und Lee analysiert und bewertet. Danach wird ein Konzept vorgestellt, mit dem man die Rauscheigenschaften von VCOs auf der Grundlage stochastischer Differentialgleichungen und Fokker-Planck-Gleichungen untersuchen kann. Ziel dieser Arbeit ist eine Beschreib...

  18. Perzeption und Produktion von Gesten bei Apraxie

    OpenAIRE

    Hovorka, Sonja Christina

    2016-01-01

    Die Dissertation untersucht Perzeption und Produktion von Gesten bei linkshirngeschädigten Patienten mit Apraxie. Die Studie führt Tests zu Pantomime, Hand-, Fingerstellungen, Aphasie und den FAT an 50 Probanden durch. Für Pantomime und Fingerstellungen kann erfolgreiche Perzeption einer Geste als Voraussetzung für ihre Produktion bestätigt werden. Schließlich wird anhand von Einzelfallanalysen die These Liepmann’s eines posterior-anterior Stroms der Handlungskontrolle von Gesten diskutiert. ...

  19. Numerische Simulation von Verbrennungslärm

    OpenAIRE

    Mühlbauer, Bernd Michael

    2012-01-01

    Instationäre Prozesse reagierender Strömungen verursachen Verbrennungslärm mit hohen Schalldruckamplituden. Direkter Verbrennungslärm wird durch turbulente Strömungsstrukturen oder periodische Verbrennungsschwingungen in der Flammenzone generiert, indirekter Verbrennungslärm entsteht hingegen durch die starke Beschleunigung von Entropiemoden oder Wirbeln, zum Beispiel in der ersten Turbinenstufe einer Gasturbine. Die numerische Simulation von sowohl direktem als auch von indirektem Verbrennun...

  20. Selektives Laserstrahlschmelzen von Titanaluminiden und Stahl

    OpenAIRE

    Löber, Lukas

    2015-01-01

    Diese Arbeit beschäftigt sich mit den aktuell bestehenden Herausforderungen der Technologie der additiven Fertigung in Form des selektiven Laserstrahlschmelzen (SLM). Es soll sich mit den Aspekten des Leichtbaus beim SLM-Verfahren beschäftigt werden. Dies geschieht mit zwei theoretischen Lösungsansätzen zur Gewichtsreduzierung von Bauteilen: 1. der Einsatz von Werkstoffen geringerer Dichte oder von neuen hochfesten Werkstoffen; 2. neue Bauweisen durch neue Konstruktions- und Werkstoffa...

  1. Wirkung von Transaminaseinhibitoren auf die Tryptophan-abhängige Pigmentbildung von Malassezia furfur

    OpenAIRE

    Vöhrs, Inga Susanne Gabriele

    2010-01-01

    Die vorliegende Arbeit untersucht die Wirkung von Transaminaseinhibitoren auf die Pigmentbildung von Malassezia furfur. Das Phänomen der Pigmentbildung von Malassezia furfur durch Verstoffwechselung der Aminosäure Tryptophan, wenn diese als alleinige Stickstoffquelle vorhanden ist, wurde 1998 erstmalig beschrieben (Mayser 1998). Diese Pigmente können verschiedene klinische Ausprägungen der Pityriasis versicolor verursac...

  2. Genetic survey of an isolated community in Bali, Indonesia. I. Blood groups, serum proteins and hepatitis B serology.

    Science.gov (United States)

    Breguet, G; Ney, R; Grimm, W; Hope, S L; Kirk, R L; Blake, N M; Narendra, I B; Toha, A

    1982-01-01

    320 adults and children of an isolated community of Bali, Indonesia, have been tested for blood groups ABO, Rh, MNS, P, Lewis, Duffy, Kell, for haptoglobin and transferrin and for hepatitis B surface antigen and antibodies. Phenotype distribution and gene frequencies are given for the total population tested and for two subgroups representative of the inbred population of the isolate and of the non-inbred part of the population. Significant differences between the two subgroups show a clear genetic drift in the inbred population. The study brings biological support to the ethnological hypothesis of population migrations in this area. Tests for hepatitis B surface antigen reveal a lower prevalence of the disease than in most other south-east Asian populations. PMID:7068159

  3. The Key Proteins of Dopaminergic Neurotransmission of Human Peripheral Blood Lymphocytes: Changed mRNA Level in Alcohol Dependence Syndrome.

    Science.gov (United States)

    Taraskina, A E; Grunina, M N; Zabotina, A M; Nasyrova, R F; Ivanov, M V; Krupitsky, E M; Schwartzman, A L

    2015-12-01

    The expression of dopamine receptor (DRD), Nurr1 transcription factor (NR4A2), and α-sinucleine (SNCA) genes in peripheral blood lymphocytes is evaluated. The results indicate that alcohol dependence is associated with high expression of SNCA and DRD4 (signifi cantly higher than in the control group) and is not associated with changes in the work of NR4A2 and DRD3 genes. The levels of DRD3 and DRD4 mRNA form a positive linear correlation (p≤0.05). The expression of SNCA and DRD4 genes can serve as an important peripheral marker of alcohol dependence development, which is essential for antipsychotic therapy. PMID:26621272

  4. Modellierung von Anwenderverhalten im Social Semantic Web

    Science.gov (United States)

    Ulbrich, Armin; Höfler, Patrick; Lindstaedt, Stefanie

    Ziel dieses Kapitels ist es, gemeinsame Verwendungsszenarien des Semantic Web und des Social Web zu identifizieren und zu benennen. Dabei wird ein Teilaspekt des Themengebiets im Detail betrachtet: die Nutzung von Services, die Beobachtungen des Verhaltens von Anwendern analysieren, um daraus maschinell interpretierbare Informationen zu erhalten und diese als Modelle zu organisieren. Es werden zunächst einige Eigenschaften und Unterscheidungsmerkmale von Anwenderverhalten und organisierten Modellen dargestellt. Anschließend wird der mögliche wechselseitige Nutzen von Anwenderverhalten und Modellen diskutiert. Den Abschluss bildet eine Betrachtung einiger exemplarischer Software-Services, die heute schon verwendet werden, um Anwenderverhalten in Modelle überzuführen.

  5. Schockwellensynthese und Charakterisierung von Aluminiumnitrid mit Kochsalzstruktur

    OpenAIRE

    Keller, Kevin

    2014-01-01

    Die vorliegende Arbeit beschreibt die Ergebnisse der Synthese und Charakterisierung der Hochdruckphase von Aluminiumnitrid mit Kochsalzstruktur (rs-AlN). Die Versuche wurden mittels Schockwellensynthese unter Verwendung der „flyer-plate-Methode“ mit anschließender Probenrückgewinnung durchgeführt. Für verschiedene Aluminiumnitridpulver mit einer Ausgangsporosität k = rho_solid/rho_porous von 1,5 bis 2,5 wurde bei einem Druck von 15 bis 43 GPa die Phasenumwandlung von der Wurtzitstruktur (w-A...

  6. Nichtlineare Rauschmodellierung von LC Tank VCOs

    Directory of Open Access Journals (Sweden)

    T. Thiessen

    2008-05-01

    Full Text Available Im Rahmen dieser Arbeit wird ein alternativer Ansatz zur Phasenrauschoptimierung von LC-Tank Oszillatoren (VCOs unter Verwendung stochastischer Differentialgleichungen vorgestellt. Zunächst werden die linearen Ansätze von Leeson, Hajimiri und Lee analysiert und bewertet. Danach wird ein Konzept vorgestellt, mit dem man die Rauscheigenschaften von VCOs auf der Grundlage stochastischer Differentialgleichungen und Fokker-Planck-Gleichungen untersuchen kann. Ziel dieser Arbeit ist eine Beschreibung des Phasenrauschens auf der Basis einer nichtlinearen Rauschmodellierung, welche Parameter eines VCOs für eine Optimierung beinhaltet. Es wurde ein Matlab-Tool erstellt und die Funktionalität anhand von Simulationen verifiziert.

  7. Nichtlineare Rauschmodellierung von LC Tank VCOs

    Science.gov (United States)

    Thiessen, T.; Bremer, J.-K.; Mathis, W.

    2008-05-01

    Im Rahmen dieser Arbeit wird ein alternativer Ansatz zur Phasenrauschoptimierung von LC-Tank Oszillatoren (VCOs) unter Verwendung stochastischer Differentialgleichungen vorgestellt. Zunächst werden die linearen Ansätze von Leeson, Hajimiri und Lee analysiert und bewertet. Danach wird ein Konzept vorgestellt, mit dem man die Rauscheigenschaften von VCOs auf der Grundlage stochastischer Differentialgleichungen und Fokker-Planck-Gleichungen untersuchen kann. Ziel dieser Arbeit ist eine Beschreibung des Phasenrauschens auf der Basis einer nichtlinearen Rauschmodellierung, welche Parameter eines VCOs für eine Optimierung beinhaltet. Es wurde ein Matlab-Tool erstellt und die Funktionalität anhand von Simulationen verifiziert.

  8. Peripheral blood mononuclear cell responses to heat shock proteins and their derived synthetic peptides in juvenile idiopathic arthritis patients

    Czech Academy of Sciences Publication Activity Database

    Sedláčková, L.; Velek, Jiří; Vavřincová, P.; Hromadníková, I.

    2006-01-01

    Roč. 55, č. 4 (2006), s. 153-159. ISSN 1023-3830 Grant ostatní: Transeurope(XE) QLK3-2002-01936; Transnet(XE) MRTN-CT-2004-512253 Institutional research plan: CEZ:AV0Z40550506 Keywords : heat shock proteins * proliferative response * juvenile idiopathic arthritis * Hsp-derived synthetic peptides Subject RIV: CC - Organic Chemistry Impact factor: 1.485, year: 2006

  9. A significant correlation between C - reactive protein levels in blood monocytes derived macrophages versus content in carotid atherosclerotic lesions

    OpenAIRE

    Kaplan, Marielle; Hamoud, Shadi; Tendler, Yevgeny; Meilin, Edna; Lazarovitch, Aviva; Nitecki, Samy; Hayek, Tony

    2014-01-01

    Background Atherosclerosis is a complex disease involving different cell types, including macrophages that play a major role in the inflammatory events occurring in atherogenesis. C-Reactive Protein (CRP) is a sensitive systemic marker of inflammation and was identified as a biomarker of cardiovascular diseases. Histological studies demonstrate CRP presence in human atherosclerotic lesions, and we have previously shown that macrophages express CRP mRNA. CRP could be locally secreted in the at...

  10. Ultra-sensitive detection of prion protein fibrils by flow cytometry in blood from cattle affected with bovine spongiform encephalopathy

    Directory of Open Access Journals (Sweden)

    Maas Elke

    2005-10-01

    Full Text Available Abstract Background The definite diagnosis of prion diseases such as Creutzfeldt-Jakob disease (CJD in humans or bovine spongiform encephalopathy (BSE in cattle currently relies on the post mortem detection of the pathological form of the prion protein (PrPSc in brain tissue. Infectivity studies indicate that PrPSc may also be present in body fluids, even at presymptomatic stages of the disease, albeit at concentrations well below the detection limits of currently available analytical methods. Results We developed a highly sensitive method for detecting prion protein aggregates that takes advantage of kinetic differences between seeded and unseeded polymerization of prion protein monomers. Detection of the aggregates was carried out by flow cytometry. In the presence of prion seeds, the association of labelled recombinant PrP monomers in plasma and serum proceeds much more efficiently than in the absence of seeds. In a diagnostic model system, synthetic PrP aggregates were detected down to a concentration of approximately 10-8 nM [0.24 fg/ml]. A specific signal was detected in six out of six available serum samples from BSE-positive cattle. Conclusion We have developed a method based on seed-dependent PrP fibril formation that shows promising results in differentiating a small number of BSE-positive serum samples from healthy controls. This method may provide the basis for an ante mortem diagnostic test for prion diseases.

  11. Tuning the pH-shift protein-isolation method for maximum hemoglobin-removal from blood rich fish muscle.

    Science.gov (United States)

    Abdollahi, Mehdi; Marmon, Sofia; Chaijan, Manat; Undeland, Ingrid

    2016-12-01

    A main challenge preventing optimal use of protein isolated from unconventional raw materials (e.g., small pelagic fish and fish by-products) using the pH-shift method is the difficulty to remove enough heme-pigments. Here, the distribution of hemoglobin (Hb) in the different fractions formed during pH-shift processing was studied using Hb-fortified cod mince. Process modifications, additives and prewashing were then investigated to further facilitate Hb-removal. The alkaline pH-shift process version could remove considerably more Hb (77%) compared to the acidic version (37%) when proteins were precipitated at pH 5.5; most Hb was removed during dewatering. Protein precipitation at pH 6.5 improved total Hb removal up to 91% and 74% during alkaline and acid processing, respectively. Adding phytic acid to the first supernatant of the alkaline process version yielded 93% Hb removal. Combining one prewash with phytic acid at pH 5.5 followed by alkaline/acid pH-shift processing increased Hb removal up to 96/92%. PMID:27374526

  12. Integrated Blood Barcode Chips

    OpenAIRE

    Fan, Rong; Vermesh, Ophir; Srivastava, Alok; Yen, Brian K.H.; Qin, Lidong; Ahmad, Habib; Kwong, Gabriel A.; Liu, Chao-Chao; Gould, Juliane; Hood, Leroy; Heath, James R.

    2008-01-01

    Blood comprises the largest version of the human proteome1. Changes of plasma protein profiles can reflect physiological or pathological conditions associated with many human diseases, making blood the most important fluid for clinical diagnostics2-4. Nevertheless, only a handful of plasma proteins are utilized in routine clinical tests. This is due to a host of reasons, including the intrinsic complexity of the plasma proteome1, the heterogeneity of human diseases and the fast kinetics assoc...

  13. Neutralization of Interleukin-10 Significantly Enhances Gamma Interferon Expression in Peripheral Blood by Stimulation with Johnin Purified Protein Derivative and by Infection with Mycobacterium avium subsp. paratuberculosis in Experimentally Infected Cattle with Paratuberculosis

    OpenAIRE

    Buza, Joram J.; Hikono, Hirokazu; Mori, Yasuyuki; Nagata, Reiko; Hirayama, Sachiyo; Bari, Abusaleh M.; Shu, Yujing; Tsuji, Noriko M.; Momotani, Eiichi

    2004-01-01

    Monoclonal antibody neutralization of interleukin-10 (IL-10) increased Johnin purified protein derivative-induced whole-blood gamma interferon (IFN-γ) secretion 23-fold and also increased IFN-γ secretion ninefold following in vitro Mycobacterium avium subsp. paratuberculosis infection of peripheral blood mononuclear cells. These results demonstrate the suppressive effect of IL-10 on immune responses to M. avium subsp. paratuberculosis infection in cattle.

  14. Effects of the Dietary Addition of Amaranth (Amaranthus mantegazzianus) Protein Isolate on Antioxidant Status, Lipid Profiles and Blood Pressure of Rats.

    Science.gov (United States)

    Lado, María B; Burini, Julieta; Rinaldi, Gustavo; Añón, María C; Tironi, Valeria A

    2015-12-01

    The effects of the dietary addition of 2.5% (w/w) Amaranthus mantegazzianus protein isolate (AI) on blood pressure, lipid profiles and antioxidative status of Wistar rats were evaluated. Six diets were used to feed animals during 28 days: (base (AIN93G), Chol (cholesterol 1%, w/w), CE (α-tocopherol 0.005%, w/w), CholE (cholesterol 1% (w/w) + α-tocopherol 0.005%, w/w), CAI (AI 2.5% w/w), CholAI (cholesterol 1% (w/w) + AI 2.5%, w/w). Lipid profiles of plasma and liver and faecal cholesterol content were analyzed. Antioxidant status was evaluated by the ferric reducing activity of plasma (FRAP), the 2-thiobarbituric acid (TBA) assay and superoxide dismutase (SOD) activity in plasma and liver. Blood pressure was measured in the tail artery of rats. CholA group presented a significant (α < 0.05) reduction (16%) in the plasma total cholesterol. In liver, the intake of cholesterol (Chol group) induced a significant increment in cholesterol and triglycerides (2.5 and 2.3 times, respectively), which could be decreased (18% and 47%, respectively) by the addition of AI (CholA group). This last group also showed an increased faecal cholesterol excretion (20%). Increment (50%) in FRAP values, diminution of TBA value in plasma and liver (70% and 38%, respectively) and diminution of SOD activity (20%) in plasma of CholA group suggest an antioxidant effect because of the intake of AI. In addition, CA and CholA groups presented a diminution (18%) of blood pressure after 28 days. PMID:26497504

  15. Expression of Cellular Isoform of Prion Protein on the Surface of Peripheral Blood Lymphocytes Among Women Exposed to Low Doses of Ionizing Radiation

    International Nuclear Information System (INIS)

    Ionizing radiation affect the expression of adhesive and co-stimulation molecules in lymphocytes. The objective of this study was to determinate the effect of low doses of ionizing radiation on the expression of prion protein PrPc on the surface peripheral blood lymphocytes in the women operating X-ray equipment. In female workers and persons of the control group the PrPc expression on CD3 (T-lymphocytes), Cd4 (T-helper), CD8 (T-cytotoxic) and CD19 (B- lymphocytes), were tested. We conclude that in women operating X-ray equipment the relationship between low doses of ionizing radiation and expression of PrPc on lymphocytes does exist concerning CD3, CD4 and CD lymphocytes. (author)

  16. Synthesis and deposition of basement membrane proteins by primary brain capillary endothelial cells in a murine model of the blood-brain barrier

    DEFF Research Database (Denmark)

    Thomsen, Maj Schneider; Birkelund, Svend; Burkhart, Annette;

    2016-01-01

    The brain vascular basement membrane is important for both blood-brain barrier (BBB) development, stability, and barrier integrity and the contribution hereto from brain capillary endothelial cells (BCECs), pericytes, and astrocytes of the BBB is probably significant. The aim of the present study......-culture, in co-culture with pericytes or mixed glial cells, or as a triple-culture with both pericytes and mixed glial cells. The integrity of the BBB models was validated by measures of transendothelial electrical resistance (TEER) and passive permeability to mannitol. The expression of basement membrane...... proteins was analysed using RT-qPCR, mass spectrometry, and immunocytochemistry. Co-culturing mBCECs with pericytes, mixed glial cells, or both significantly increased the TEER compared to the mono-culture, and a low passive permeability was correlated with high TEER. The mBCECs expressed all major...

  17. Ubiquitous Computing: Von der Vernetzung von Computern zur Vernetzung von Dingen

    OpenAIRE

    Christ, Oliver; Fleisch, Elgar

    2003-01-01

    In den letzten Jahren haben Unternehmen neben der Einführung ihrer Enterprise Ressource Planning Systeme integrierte Schnittstellen zu Kunden und Lieferanten geschaffen und auf diesem Weg die Vernetzung der einzelnen Unternehmen realisiert [vgl. Fleisch 2001]. Zunehmend beginnen Unternehmen mobile Anwendungen zu entwickeln, urn Mitarbeitern, Kunden und Lieferanten ortsunabhängigen Zugang zu ihren Informationssystemen zu ermöglichen. Wenn heute von "mobile commerce" gesprochen wird, dann geht ...

  18. Expression of tight junction proteins and transporters for xenobiotic metabolism at the blood-CSF barrier during development in the nonhuman primate (P. hamadryas).

    Science.gov (United States)

    Ek, C Joakim; D'Angelo, Barbara; Lehner, Christine; Nathanielsz, Peter; Li, Cun; Mallard, Carina

    2015-08-15

    The choroid plexus (CP) is rich in barrier mechanisms including transporters and enzymes which can influence drug disposition between blood and brain. We have limited knowledge of their state in fetus. We have studied barrier mechanisms along with metabolism and transporters influencing xenobiotics, using RNAseq and protein analysis, in the CP during the second-half of gestation in a nonhuman primate (Papio hamadryas). There were no differences in the expression of the tight-junctions at the CP suggesting a well-formed fetal blood-CSF barrier during this period of gestation. Further, the fetal CP express many enzymes for phase I-III metabolisms as well as transporters suggesting that it can greatly influence drug disposition and has a significant machinery to deactivate reactive molecules with only minor gestational changes. In summary, the study suggests that from, at least, midgestation, the CP in the nonhuman primate is restrictive and express most known genes associated with barrier function and transport. PMID:26092209

  19. Human Neutrophil Elastase Induce Interleukin-10 Expression in Peripheral Blood Mononuclear Cells through Protein Kinase C Theta/Delta and Phospholipase Pathways

    Directory of Open Access Journals (Sweden)

    Jin Kawata

    2016-02-01

    Full Text Available Objective: Neutrophils have an important role in the rapid innate immune response, and the release or active secretion of elastase from neutrophils is linked to various inflammatory responses. Purpose of this study was to determine how the human neutrophil elastase affects the interleukin-10 (IL-10 response in peripheral blood mononuclear cells (PBMC. Materials and Methods: In this prospective study, changes in IL-10 messenger RNA (mRNA and protein expression levels in monocytes derived from human PBMCs were investigated after stimulation with human neutrophil elastase (HNE. A set of inhibitors was used for examining the pathways for IL-10 production induced by HNE. Results: Reverse transcription polymerase chain reaction (RT-PCR showed that stimulation with HNE upregulated IL-10 mRNA expression by monocytes, while the enzyme-linked immunosorbent assay (ELISA revealed an increase of IL-10 protein level in the culture medium. A phospholipase C inhibitor (U73122 partially blunted the induction of IL-10 mRNA expression by HNE, while IL-10 mRNA expression was significantly reduced by a protein kinase C (PKC inhibitor (Rottlerin. A calcium chelator (3,4,5-trimethoxybenzoic acid 8-(diethylaminooctyl ester: TMB-8 inhibited the response of IL-10 mRNA to stimulation by HNE. In addition, pretreatment with a broad-spectrum PKC inhibitor (Ro-318425 partly blocked the response to HNE. Finally, an inhibitor of PKC theta/delta abolished the increased level of IL-10 mRNA expression. Conclusion: These results indicate that HNE mainly upregulates IL-10 mRNA expression and protein production in moncytes via a novel PKC theta/delta, although partially via the conventional PKC pathway.

  20. Human Neutrophil Elastase Induce Interleukin-10 Expression in Peripheral Blood Mononuclear Cells through Protein Kinase C Theta/Delta and Phospholipase Pathways

    Science.gov (United States)

    Kawata, Jin; Yamaguchi, Rui; Yamamoto, Takatoshi; Ishimaru, Yasuji; Sakamoto, Arisa; Aoki, Manabu; Kitano, Masafumi; Umehashi, Misako; Hirose, Eiji; Yamaguchi, Yasuo

    2016-01-01

    Objective Neutrophils have an important role in the rapid innate immune response, and the release or active secretion of elastase from neutrophils is linked to various inflammatory responses. Purpose of this study was to determine how the human neutrophil elastase affects the interleukin-10 (IL-10) response in peripheral blood mononuclear cells (PBMC). Materials and Methods In this prospective study, changes in IL-10 messenger RNA (mRNA) and protein expression levels in monocytes derived from human PBMCs were investigated after stimulation with human neutrophil elastase (HNE). A set of inhibitors was used for examining the pathways for IL-10 production induced by HNE. Results Reverse transcription polymerase chain reaction (RT-PCR) showed that stimulation with HNE upregulated IL-10 mRNA expression by monocytes, while the enzyme-linked immunosorbent assay (ELISA) revealed an increase of IL-10 protein level in the culture medium. A phospholipase C inhibitor (U73122) partially blunt- ed the induction of IL-10 mRNA expression by HNE, while IL-10 mRNA expression was significantly reduced by a protein kinase C (PKC) inhibitor (Rottlerin). A calcium chelator (3,4,5-trimethoxybenzoic acid 8-(diethylamino)octyl ester: TMB-8) inhibited the response of IL-10 mRNA to stimulation by HNE. In addition, pretreatment with a broad-spectrum PKC inhibitor (Ro-318425) partly blocked the response to HNE. Finally, an inhibitor of PKC theta/delta abolished the increased level of IL-10 mRNA expression. Conclusion These results indicate that HNE mainly upregulates IL-10 mRNA ex- pression and protein production in moncytes via a novel PKC theta/delta, although partially via the conventional PKC pathway. PMID:26862528

  1. Acid-labile protein-adducted heterocyclic aromatic amines in human blood are not viable biomarkers of dietary exposure: A systematic study.

    Science.gov (United States)

    Cooper, Kevin M; Brennan, Sarah F; Woodside, Jayne V; Cantwell, Marie; Guo, Xiaoxiao; Mooney, Mark; Elliott, Christopher T; Cuskelly, Geraldine J

    2016-05-01

    Heterocyclic aromatic amines (HCA) are carcinogenic mutagens formed during cooking of protein-rich foods. HCA residues adducted to blood proteins have been postulated as biomarkers of HCA exposure. However, the viability of quantifying HCAs following hydrolytic release from adducts in vivo and correlation with dietary intake are unproven. To definitively assess the potential of labile HCA-protein adducts as biomarkers, a highly sensitive UPLC-MS/MS method was validated for four major HCAs: 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP), 2-amino-3,8-dimethylimidazo[4,5-f]quinoxaline (MeIQx), 2-amino-3,4,8-trimethylimidazo[4,5-f]quinoxaline (4,8-DiMeIQx) and 2-amino-3,7,8-trimethylimidazo[4,5-f]quinoxaline (7,8-DiMeIQx). Limits of detection were 1-5 pg/ml plasma and recoveries 91-115%. Efficacy of hydrolysis was demonstrated by HCA-protein adducts synthesised in vitro. Plasma and 7-day food diaries were collected from 122 fasting adults consuming their habitual diets. Estimated HCA intakes ranged from 0 to 2.5 mg/day. An extensive range of hydrolysis conditions was examined for release of adducted HCAs in plasma. HCA was detected in only one sample (PhIP, 9.7 pg/ml), demonstrating conclusively for the first time that acid-labile HCA adducts do not reflect dietary HCA intake and are present at such low concentrations that they are not feasible biomarkers of exposure. Identification of biomarkers remains important. The search should concentrate on stabilised HCA-peptide markers and use of untargeted proteomic and metabolomic approaches. PMID:26993956

  2. Lower HIV provirus levels are associated with more APOBEC3G protein in blood resting memory CD4+ T lymphocytes of controllers in vivo.

    Directory of Open Access Journals (Sweden)

    Mariapia De Pasquale

    Full Text Available Immunodeficiency does not progress for prolonged periods in some HLA B57- and/or B27-positive subjects with human immunodeficiency virus type 1 (HIV infection, even in the absence of antiretroviral therapy (ART. These "controllers" have fewer HIV provirus-containing peripheral blood mononuclear cells than "non-controller" subjects, but lymphocytes that harbor latent proviruses were not specifically examined in studies to date. Provirus levels in resting memory cells that can serve as latent reservoirs of HIV in blood were compared here between controllers and ART-suppressed non-controllers. APOBEC3G (A3G, a cellular factor that blocks provirus formation at multiple steps if not antagonized by HIV virion infectivity factor (Vif, was also studied. HLA-linked HIV control was associated with less provirus and more A3G protein in resting CD4+ T central memory (Tcm and effector memory (Tem lymphocytes (provirus: p = 0.01 for Tcm and p = 0.02 for Tem; A3G: p = 0.02 for Tcm and p = 0.02 for Tem. Resting memory T cells with the highest A3G protein levels (>0.5 RLU per unit of actin had the lowest levels of provirus (<1,000 copies of DNA per million cells in vivo (p = 0.03, Fisher's exact test. Using two different experimental approaches, Vif-positive viruses with more A3G were found to have decreased virion infectivity ex vivo. These results raise the hypothesis that HIV control is associated with increased cellular A3G that may be packaged into Vif-positive virions to add that mode of inhibition of provirus formation to previously described adaptive immune mechanisms for HIV control.

  3. Differential localization of P-selectin and von Willebrand factor during megakaryocyte maturation

    DEFF Research Database (Denmark)

    Zingariello, M; Fabucci, M E; Bosco, D;

    2010-01-01

    An important step in megakaryocyte maturation is the appropriate assembly of at least two distinct subsets of alpha-granules. The mechanism that sorts the alpha-granule components into distinct structures and mediates their release in response to specific stimuli is now emerging. P-selectin and von...... Willebrand factor are two proteins present in the alpha-granules that recognize P-selectin glycoprotein ligand on neutrophils and collagen in the subendothelial matrix. These proteins may play an important role in determining the differential release of the alpha-granule contents in response to external...... stimuli. If P-selectin and von Willebrand factor are localized in the same or different alpha-granules is not known. To clarify this question, we analyzed by immunoelectron microscopy the localization of von Willebrand factor and P-selectin during the maturation of wild-type and Gata1(low) megakaryocytes...

  4. Improving quality of components and protein preparations of blood plasma by introduction of quality management according to the principles of GMP and ISO 9001 in the blood service institutions with benchmarking effectiveness

    Directory of Open Access Journals (Sweden)

    Liubchack V.V.

    2014-09-01

    Full Text Available Aspects of harmonious introduc¬tion of the new standards of quality management in the institutions of blood service were considered. It was proved that it does not contradict to current legislation and significantly increases pathogenic security of blood components and blood products.

  5. The CentriMag centrifugal blood pump as a benchmark for in vitro testing of hemocompatibility in implantable ventricular assist devices.

    Science.gov (United States)

    Chan, Chris H H; Pieper, Ina Laura; Hambly, Rebecca; Radley, Gemma; Jones, Alyssa; Friedmann, Yasmin; Hawkins, Karl M; Westaby, Stephen; Foster, Graham; Thornton, Catherine A

    2015-02-01

    Implantable ventricular assist devices (VADs) have proven efficient in advanced heart failure patients as a bridge-to-transplant or destination therapy. However, VAD usage often leads to infection, bleeding, and thrombosis, side effects attributable to the damage to blood cells and plasma proteins. Measuring hemolysis alone does not provide sufficient information to understand total blood damage, and research exploring the impact of currently available pumps on a wider range of blood cell types and plasma proteins such as von Willebrand factor (vWF) is required to further our understanding of safer pump design. The extracorporeal CentriMag (Thoratec Corporation, Pleasanton, CA, USA) has a hemolysis profile within published standards of normalized index of hemolysis levels of less than 0.01 g/100 L at 100 mm Hg but the effect on leukocytes, vWF multimers, and platelets is unknown. Here, the CentriMag was tested using bovine blood (n = 15) under constant hemodynamic conditions in comparison with a static control for total blood cell counts, hemolysis, leukocyte death, vWF multimers, microparticles, platelet activation, and apoptosis. The CentriMag decreased the levels of healthy leukocytes (P pump which could be used as a standard in blood damage assays to inform the design of new implantable blood pumps. PMID:25066768

  6. Studien der Genexpressionsänderung während der Entwicklung von Drosophila melanogaster mittels DNA-Microarrays

    OpenAIRE

    Beckmann, Boris

    2003-01-01

    Die Fruchtfliege Drosophila melanogaster ist einer der am besten untersuchten Modellorganismen in der Entwicklungsbiologie. Die Kenntnis des Genoms und die Zahl der annotierten protein-kodierenden Gene von Drosophila sind jedoch Schwankungen unterworfen, da sie meist auf Computerberechnungen bereits bekannter Gene und nur teilweise auf experimentellen Nachweisen beruhen. Um das Transkriptom von Drosophila im Verlauf der Entwicklung zu untersuchen und neue potentielle Gene zu identifizieren wu...

  7. Low-protein vegetarian diet does not have a short-term effect on blood acid–base status but raises oxygen consumption during submaximal cycling

    Directory of Open Access Journals (Sweden)

    Hietavala Enni-Maria

    2012-11-01

    Full Text Available Abstract Background Acid–base balance refers to the equilibrium between acids and bases in the human body. Nutrition may affect acid–base balance and further physical performance. With the help of PRAL (potential renal acid load, a low-protein vegetarian diet (LPVD was designed to enhance the production of bases in body. The aim of this study was to investigate if LPVD has an effect on blood acid–base status and performance during submaximal and maximal aerobic cycling. Methods Nine healthy, recreationally active men (age 23.5 ± 3.4 yr participated in the study and were randomly divided into two groups in a cross-over study design. Group 1 followed LPVD for 4 days and group 2 ate normally (ND before performing a cycle ergometer test. The test included three 10-min stages at 40, 60 and 80% of VO2max. The fourth stage was performed at 100% of VO2max until exhaustion. After 10–16 days, the groups started a second 4-day diet, and at the end performed the similar ergometer test. Venous blood samples were collected at the beginning and at the end of both diet periods and after every stage cycled. Results Diet caused no significant difference in venous blood pH, strong ion difference (SID, total concentration of weak acids (Atot, partial pressure of CO2 (pCO2 or HCO3- at rest or during cycling between LPVD and ND. In the LPVD group, at rest SID significantly increased over the diet period (38.6 ± 1.8 vs. 39.8 ± 0.9, p=0.009. Diet had no significant effect on exercise time to exhaustion, but VO2 was significantly higher at 40, 60 and 80% of VO2max after LPVD compared to ND (2.03 ± 0.25 vs. 1.82 ± 0.21 l/min, p=0.035; 2.86 ± 0.36 vs. 2.52 ± 0.33 l/min, p Conclusion There was no difference in venous blood acid–base status between a 4-day LPVD and ND. VO2 was increased during submaximal cycling after LPVD suggesting that the exercise economy was poorer. This had no further effect on maximal aerobic performance. More studies are needed to

  8. BASED TO CLINICAL CASE. VON HIPLEA-LINDAU SYNDROME

    Directory of Open Access Journals (Sweden)

    Brzeziński Piotr

    2011-01-01

    Full Text Available von Hippel-Lindau syndrome (VHL is a rare, genetic multi-system disorder characterized by the abnormal growth of tumors in certain parts of the body (angiomatosis. The tumors of the central nervous system (CNS are benign and are comprised of a nest of blood vessels and are called hemangioblastomas. Hemangioblastomas may develop in the brain, the retina of the eyes, and other areas of the nervous system. Other types of tumors develop in the adrenal glands, the kidneys, or the pancreas. Symptoms of VHL vary among patients and depend on the size and location of the tumors. Symptoms may include headaches, problems with balance and walking, dizziness, weakness of the limbs, vision problems, and high blood pressure. Cysts (fluid-filled sacs and/or tumors (benign or cancerous may develop around the hemangioblastomas and cause the symptoms listed above. Individuals with VHL are also at a higher risk than normal for certain types of cancer, especially kidney cancer. Based on the case of 30-year old patient with characteristics of von Hippel-Lindau syndrome as phakomatosis.

  9. Walt Disney and Dr. Wernher von Braun

    Science.gov (United States)

    1954-01-01

    Dr. Werhner von Braun, then Chief, Guided Missile Development Operation Division at Army Ballistic Missile Agency (ABMA) in Redstone Arsenal, Alabama, was visited by Walt Disney in 1954. In the 1950's, von Braun worked with Disney Studio as a technical director, making three films about space exploration for television. A model of the V-2 rocket is in background.

  10. Von Neumann was not a Quantum Bayesian.

    Science.gov (United States)

    Stacey, Blake C

    2016-05-28

    Wikipedia has claimed for over 3 years now that John von Neumann was the 'first quantum Bayesian'. In context, this reads as stating that von Neumann inaugurated QBism, the approach to quantum theory promoted by Fuchs, Mermin and Schack. This essay explores how such a claim is, historically speaking, unsupported. PMID:27091166

  11. John von Neumann and Self-Reference ...

    OpenAIRE

    Rosinger, Elemer Elad

    2012-01-01

    It is shown that the description as a "frog" of John von Neumann in a recent item by the Princeton celebrity physicist Freeman Dyson does among others miss completely on the immesnely important revolution of the so called "von Neumann architecture" of our modern electronic digital computers.

  12. Von Neumann Was Not a Quantum Bayesian

    OpenAIRE

    Blake C. Stacey

    2014-01-01

    Wikipedia has claimed for over three years now that John von Neumann was the "first quantum Bayesian." In context, this reads as stating that von Neumann inaugurated QBism, the approach to quantum theory promoted by Fuchs, Mermin and Schack. This essay explores how such a claim is, historically speaking, unsupported.

  13. Steering projections in von Neumann algebras

    OpenAIRE

    Adam Wegert

    2015-01-01

    A steering projection of an arbitrary von Neumann algebra is introduced. It is shown that a steering projection always exists and is unique (up to Murray-von Neumann equivalence). A general decomposition of arbitrary projections with respect to a steering projection is established.

  14. Von Neumann Was Not a Quantum Bayesian

    CERN Document Server

    Stacey, Blake C

    2014-01-01

    Wikipedia has claimed for over two years now that John von Neumann was the "first quantum Bayesian." In context, this reads as stating that von Neumann inaugurated QBism, the approach to quantum theory promoted by Fuchs, Mermin and Schack. This essay explores how such a claim is, historically speaking, unsupported.

  15. Acylierung von Lysinen: Erweiterung des Repertoires

    DEFF Research Database (Denmark)

    Olsen, Christian A.

    2012-01-01

    Spuren hinterlassen: Die Zahl der bekannten posttranslatorischen Modifikationen von Lysinresten hat sich dank jüngster Studien beträchtlich erhöht. Neben der Acetylierung der Aminogruppe von Lysinresten wurde eine Serie anderer Acylierungen (Crotonylierung, Succinylierung, Malonylierung) in Histo...

  16. Regulatory and junctional proteins of the blood-testis barrier in human Sertoli cells are modified by monobutyl phthalate (MBP) and bisphenol A (BPA) exposure.

    Science.gov (United States)

    de Freitas, André Teves Aquino Gonçalves; Ribeiro, Mariana Antunes; Pinho, Cristiane Figueiredo; Peixoto, André Rebelo; Domeniconi, Raquel Fantin; Scarano, Wellerson R

    2016-08-01

    The blood-testis barrier (BTB) is responsible for providing a protected environment and coordinating the spermatogenesis. Endocrine disruptors (EDs) might lead to infertility, interfering in the BTB structure and modulation. This study aimed to correlate the actions of two EDs, monobutyl phthalate (MBP) and bisphenol A (BPA) in different periods of exposure, in a low toxicity dose to the human Sertoli cells (HSeC) and its effects on the proteins of the BTB and regulatory proteins involved in its modulation. HSeC cells were exposed to MBP (10μM) and BPA (20μM) for 6 and 48h. Western Blot assay indicated that MBP was able to reduce the expression of occludin, ZO-1, N-cadherin and Androgen Receptor (AR), while BPA leads to a reduction of occludin, ZO-1, β-catenin and AR. TGF-β2 and F-actin were not modified. Phalloidin and Hematoxylin and Eosin assay revealed phenotically disruption in Sertoli cells adhesion, without changes in F-actin expression or localization. Our data suggested both EDs present potential for disrupting the structure and maintenance of the human BTB by AR dependent pathway. PMID:26922907

  17. Abnormal activation of calpain and protein kinase Cα promotes a constitutive release of matrix metalloproteinase 9 in peripheral blood mononuclear cells from cystic fibrosis patients.

    Science.gov (United States)

    Averna, Monica; Bavestrello, Margherita; Cresta, Federico; Pedrazzi, Marco; De Tullio, Roberta; Minicucci, Laura; Sparatore, Bianca; Salamino, Franca; Pontremoli, Sandro; Melloni, Edon

    2016-08-15

    Matrix metalloproteinase 9 (MMP9) is physiologically involved in remodeling the extracellular matrix components but its abnormal release has been observed in several human pathologies. We here report that peripheral blood mononuclear cells (PBMCs), isolated from cystic fibrosis (CF) patients homozygous for F508del-cystic fibrosis transmembrane conductance regulator (CFTR), express constitutively and release at high rate MMP9 due to the alteration in their intracellular Ca(2+) homeostasis. This spontaneous and sustained MMP9 secretion may contribute to the accumulation of this protease in fluids of CF patients. Conversely, in PBMCs isolated from healthy donors, expression and secretion of MMP9 are undetectable but can be evoked, after 12 h of culture, by paracrine stimulation which also promotes an increase in [Ca(2+)]i. We also demonstrate that in both CF and control PBMCs the Ca(2+)-dependent MMP9 secretion is mediated by the concomitant activation of calpain and protein kinase Cα (PKCα), and that MMP9 expression involves extracellular signal-regulated protein kinases 1 and 2 (ERK1/2) phosphorylation. Our results are supported by the fact that either the inhibition of Ca(2+) entry or chelation of [Ca(2+)]i as well as the inhibition of single components of the signaling pathway or the restoration of CFTR activity all promote the reduction of MMP9 secretion. PMID:27349634

  18. Seasonal changes in blood serum protein fractions and in activity of AspAT and AlAT in Arabian brood mares and their foals.

    Science.gov (United States)

    Gill, J; Jakubów, K; Kompanowska-Jezierska, E; Kott, A; Szumska, D

    1985-01-01

    In 34 pure breed Arabian horses divided into four groups (Gr. I--10 pregnant mares, Gr. II--7 barren mares, Gr. III--10 foals born in 1981, Gr. IV--7 foals born in 1982) seasonal changes in total blood serum protein, its electrophoretic fractions and the activity of AspAT and AlAT were studied. Seasonal cyclicity was found in all groups in the amount of total serum proteins, and alpha 2- and beta 1-globulin fractions. Cyclicity was found in the level of albumin and activity of AspAT in three groups, not Gr. II, and in gamma-globulin, not Gr. IV. beta 2-globulin and AlAT cyclicity was found in two groups and alpha 1-globulin cyclicity was found only in Gr. II. Out of nine indices studied, cyclicity was found in eight of them in pregnant mares, Gr. I; in seven in older foals, Gr. III; in six in the young foals, Gr. IV; and in five in barren mares, Gr. II. PMID:2864198

  19. Mycoplasma hyopneumoniae-derived lipid-associated membrane proteins induce inflammation and apoptosis in porcine peripheral blood mononuclear cells in vitro.

    Science.gov (United States)

    Bai, Fangfang; Ni, Bo; Liu, Maojun; Feng, Zhixin; Xiong, Qiyan; Shao, Guoqing

    2015-01-30

    Mycoplasma hyopneumoniae is the causative agent of swine enzootic pneumonia (EP), a disease that causes considerable economic losss in swine industry. Lipid-associated membrane proteins (LAMPs) of mycoplasma play important roles in causing mycoplasma diseases. The present study explores the pathogenic mechanisms of M. hyopneumoniae LAMPs by elucidating their role in modulating the inflammation, apoptosis, and relevant signaling pathways of peripheral blood mononuclear cells (PBMCs) of pig. LAMP treatment inhibited the growth of PBMCs. Up-regulation of cytokines, such as IL-6 and IL-1β, as well as increased production of nitric oxide (NO) and superoxide anion were all detected in the supernatant of LAMPs-treated PBMCs. Furthermore, flow cytometric analysis using dual staining with annexin-V-FITC and propidium iodide (PI) showed that LAMPs of M. hyopneumoniae induced a time-dependent apoptosis in lymphocyts and monocytes from PBMCs, which was blocked by NOS inhibitor or antioxidant. In addition, LAMPs induced the phosphorylation of p38, the ratio of pro-apoptotic Bax protein to anti-apoptotic Bcl-2, activation of caspase-3 and caspase-8, and poly ADP-ribose polymerase (PARP) cleavage in PBMCs. These findings demonstrated that M. hyopneumoniae LAMPs induced the production of proinflammatory cytokines, NO and reactive oxygen species (ROS), and apoptosis of PBMCs in vitro through p38 MAPK and Bax/Bcl-2 signaling pathways, as well as caspase activation. PMID:25481242

  20. Raw and extruded pea (Pisum sativum and lupin (Lupinus albusvar. Multitalia seeds as protein sources in weaned piglets’ diets: effect on growth rate and blood parameters

    Directory of Open Access Journals (Sweden)

    Gianfranco Piva

    2010-01-01

    Full Text Available The 42 days trial was carried out using 140 piglets weaned at 28 days of age. The piglets were allocated according to  weight and sex to the 5 dietary treatments with 7 replicates for each treatments (4 pens x 4 castrated males and 3 pens  x 4 females. The piglets were fed according to the following experimental design: 1 control diet (CTR with soybean  meal (SBM 44% c.p. as protein source; 2 CRT diets with 200 g/kg of raw pea (Pisum sativum (RP; 3 CTR diet with  200 g/kg extruded pea (EP; 4 CRT diet with 170 g/kg raw lupin (Lupinus albusvar. Multitalia (RL; 5 CTR diet with  170 g/kg of extruded lupin (EL. During the trial, animals were weighed at 0 - 21 and 42 days from the start of the trial.  Feed intake was monitored and feed conversion ratio was calculated for the periods 0-21 d and 22-42 d. At the end of  the trial, blood samples were taken for 14 animals for each dietary treatment (2 animals per replicate and analysed for  total protein, urea and liver activity (ALT, AST and ALP parameters. Average daily weight gain and feed intake did not  differ according to dietary treatments whereas during the total experimental period (0-42 d, feed conversion ratio was  higher for EP vsCTR diet (2.35 vs2.09, respectively; P   compared with diets containing the raw ingredients did not differ. Feed conversion ratio for the RP was numerically high-  er than for the EP (2.35 vs2.16 and 2.76 vs2.32, respectively during 22-42 d and 0-42 d periods. Blood parameters  did not show significant difference among dietary treatments except for higher total protein for CTR diet vsRL diet, EL  and RP (67.3 vs62.2, 62.8 and 63.6 g/l, respectively; PvsRL  and RL (4.7 vs3.7 and 3.8 mmol/l respectively; P 

  1. Molecular mechanisms of gene activation and gene expression mediated by CCAAT/enhancer binding proteins

    OpenAIRE

    Zaragoza Dörr, Katrin

    2008-01-01

    Der Transkriptionsfaktor CCAAT/Enhancer-Binding Protein alpha (C/EBPa) koordiniert Proliferationshemmung und Differenzierung von myeloiden VorlŠuferzellen und Adipozyten. C/EBPa ist ein transkriptioneller Aktivator von abstammungspezifischen Genen und blockiert den Zellzyklus durch Repression von proliferationsfšrdernden E2F Zielgenen. Die hier gezeigten Daten zeigen, dass auch umgekehrt E2F die transkriptionelle und differenzierungsfšrdernde AktivitŠt von C/EBPa entgegenwirkt. Somit besitzen...

  2. Franz Xaver von Zach in England

    Science.gov (United States)

    McConnell, Anita

    F.X. von Zach arrived in London in November 1783, and was employed as tutor to George, son of Count von Brühl, the Saxonian commercial diplomat. Von Zach became a part of von Brühl's household, and was thus in close contact with the leading astronomers, horologists, and scientific instrument makers. Before he departed to Gotha in 1788, he was awarded an honorary degree by Oxford University under false pretences, he upset the Astronomer Royal, and was twice rejected by the Royal Society. At von Brühl's country house he was shown the manuscripts of the mathematician Thomas Harriot (d. 1621); but his attempts to publicise them were hampered by his misreadings, and his treatment of the documents caused confusion in England's scientific community, which lasted throughout his lifetime.

  3. Komplexierung von Nickelionen an Pektin

    OpenAIRE

    Schauer, Anke

    2001-01-01

    Mobilität und Bioverfügbarkeit toxischer Metallionen in pflanzlichen Organismen sind wichtige Fragen der aktuellen Umweltchemie, da durch direkten anthropogenen Eintrag und durch Mobilisierung von natürlich vorliegenden Mineralien (aufgrund des sauren Regens oder natürlicher pH-Wert Absenkung in Böden) toxische Metallionen wie Cadmium-, Nickel- oder auch Kupferionen in hohen Konzentrationen für Pflanzen verfügbar werden und damit in die Nahrungskette gelangen. Es existieren zahlreiche Spezies...

  4. Numerische Beschreibung von dynamischen Brennkammersystemen

    OpenAIRE

    Liu, Martin

    2009-01-01

    In der vorliegenden Arbeit wurde mit dem neuentwickelten Berechnungsverfahren DyNe ein Strömungslöser für instationäre, eindimensionale, nicht reagierende Strömungen entwickelt. Ziel war es, durch Kopplung dieses Verfahrens mit einem CFD-Löser für reagierende Strömungen das dynamische Verhalten von Brennkammersystemen zu simulieren. Der 1D-Löser DyNe sollte dabei akustische und strömungstechnische Phänomene in den Luft- und Brennstoffzuleitungen des Brennkammersystems, dem dynamischen Netzwer...

  5. Blood Types

    Science.gov (United States)

    ... How Can I Help a Friend Who Cuts? Blood Types KidsHealth > For Teens > Blood Types Print A A ... or straight hair instead of curly. ...Make Eight Blood Types The different markers that can be found in ...

  6. Blood Types

    Science.gov (United States)

    ... confidence to respond in emergency situations with the skills that can help to save a life. Learn more » Red Cross Information Donating Blood Learn About Blood Hosting a Blood Drive For Hospitals Engage with Us About Us Media ...

  7. Identification of a Small Molecule That Modulates Platelet Glycoprotein Ib-von Willebrand Factor Interaction*

    Science.gov (United States)

    Broos, Katleen; Trekels, Mieke; Jose, Rani Alphonsa; Demeulemeester, Jonas; Vandenbulcke, Aline; Vandeputte, Nele; Venken, Tom; Egle, Brecht; De Borggraeve, Wim M.; Deckmyn, Hans; De Maeyer, Marc

    2012-01-01

    The von Willebrand factor (VWF) A1-glycoprotein (GP) Ibα interaction is of major importance during thrombosis mainly at sites of high shear stress. Inhibitors of this interaction prevent platelet-dependent thrombus formation in vivo, without major bleeding complications. However, the size and/or protein nature of the inhibitors currently in development limit oral bioavailability and clinical development. We therefore aimed to search for a small molecule protein-protein interaction inhibitor interfering with the VWF-GPIbα binding. After determination of putative small molecule binding pockets on the surface of VWF-A1 and GPIbα using site-finding algorithms and molecular dynamics, high throughput molecular docking was performed on both binding partners. A selection of compounds showing good in silico docking scores into the predicted pockets was retained for testing their in vitro effect on VWF-GPIbα complex formation, by which we identified a compound that surprisingly stimulated the VWF-GPIbα binding in a ristocetin cofactor ELISA and increased platelet adhesion in whole blood to collagen under arterial shear rate but in contrast inhibited ristocetin-induced platelet aggregation. The selected compound adhering to the predicted binding partner GPIbα could be confirmed by saturation transfer difference NMR spectroscopy. We thus clearly identified a small molecule that modulates VWF-GPIbα binding and that will now serve as a starting point for further studies and chemical modifications to fully characterize the interaction and to manipulate specific activity of the compound. PMID:22232560

  8. Immunoelectrophoresis - blood

    Science.gov (United States)

    IEP - serum; Immunoglobulin electrophoresis - blood; Gamma globulin electrophoresis; Serum immunoglobulin electrophoresis ... A blood sample is needed. For information on how this is done, see: Venipuncture

  9. Pregnancy Loss in Dairy Cattle: Relationship of Ultrasound, Blood Pregnancy-Specific Protein B, Progesterone and Production Variables.

    Science.gov (United States)

    Gábor, G; Kastelic, J P; Abonyi-Tóth, Z; Gábor, P; Endrődi, T; Balogh, O G

    2016-08-01

    Objectives were to determine associations between percentage pregnancy loss (PPL) in dairy cattle and: (i) pregnancy diagnosis by ultrasonography; (ii) pregnancy diagnosis by serum pregnancy-specific protein B (PSPB) concentrations, with or without serum progesterone concentrations; and (iii) production and environmental factors. This study included 149 822 pregnancy diagnoses conducted over 13 years in Holstein-Friesian cows in Hungarian dairy herds. The following were determined: PPL in cows diagnosed pregnant by transrectal ultrasonography 29-42 days after artificial insemination (AI; n = 11 457); PPL in cows diagnosed pregnant by serum PSPB 29-35 days after AI (n = 138 365); and PPL and its association with serum progesterone concentrations, PSPB and production/environmental variables. The definition of PPL was percentage of cows initially diagnosed pregnant based on ultrasonography or PSPB, but not pregnant when examined by transrectal palpation 60 -70 days after AI. The PPL was lower (p 1.1 ng/ml) was lowest (15.0%), whereas cows with low concentrations of both PSPB and progesterone (0.6-1.1 and <2 ng/ml, respectively) had the highest PPL (76.3%; p < 0.0001). Furthermore, PPL was higher in cows with advanced parity and with high milk production, when ambient temperatures were high, although body condition score (BCS) had no effect on PPL. Finally, there were no significant associations between serum PSPB and environmental temperatures or number of post-partum uterine treatments. PMID:27198072

  10. Beiträge zur Geologie von Celebes

    NARCIS (Netherlands)

    Bücking, H.

    1902-01-01

    Die Gesteine, über welche ich im Folgenden berichte, sind in den Jahren 1900 u. 1901 von Herrn H. T. HOVEN, Königl. Niederländischem Marine-Officier, Commandanten von „H. M. opnemingsvaartuig Makassar” bei Gelegenheit von Vermessungsarbeiten längs der Westküste von Celebes gesammelt und Herrn Profes

  11. Methylierung von anorganischem Quecksilber im Intestinaltrakt des Kompostwurms Eisenia foetida

    OpenAIRE

    Knopf, Burkhard

    2010-01-01

    Im Rahmen dieser Arbeit wurde die Methylierung von Quecksilber in Intestinaltrakt des Kompostwurms Eisenia foetida untersucht. Des Weiteren wurden aerobe und anaerobe Mikroorganismen aus dem Darmtrakt von Eisenia fotida isoliert, identifiziert und auf ihr Potential zur Methylierung von Quecksilber getestet. Die Bestimmung von Methylquecksilber erfolgte mittels GC-ICPMS (Gaschromatographie mit induktiv gekoppelter Plasma-Massenspektrometrie) und GC-AFS (Gaschromatographie- Atomfluoreszenzspekt...

  12. Untersuchungen zur Molekularbiologie von Escherichia coli-Wildstämmen

    OpenAIRE

    Homburg, Stefan

    2007-01-01

    Eine eindeutige Unterscheidung zwischen extraintestinal pathogenen (ExPEC) und kommensalen E. coli-Stämmen zu treffen, fällt häufig schwer, da Virulenz-assoziierte Faktoren von ExPEC auch in kommensalen Stämmen gefunden werden können. Als naher Verwandter des uropathogenen Isolates E. coli CFT073 weist der apathogene, kommensale Stamm E. coli Nissle 1917 (O6:K5:H1) die Expression einer Vielzahl solcher „ExPEC-Virulenzfaktoren“ auf. Dazu gehören verschiedene Fimbrien, Siderophore und Proteine,...

  13. Selbstorganisierende Karten zur Erkennung von Polypharmakologie in der Wirkstoffentwicklung

    OpenAIRE

    Achenbach, Janosch

    2015-01-01

    Polypharmakologie hat in den letzten Jahren mehr und mehr an Bedeutung in der pharmazeutischen Forschung gewonnen und könnte in Zukunft zu einem Umdenken in der Entwicklung neuer Wirkstoffe führen. Das wachsende Verständnis für biologische Zusammenhänge, im speziellen für die starke Vernetzung zwischen verschiedenen Signalwegen oder Gewebearten, und die daran beteiligten Proteine, könnten zu gänzlich neuen Strategien führen. Beispiele aus dem Bereich der Onkologie und der Entwicklung von Neur...

  14. Chemische und biochemische Synthesen zur Funktionsuntersuchung von Membranproteinen

    OpenAIRE

    Mak, Ellen

    2014-01-01

    Die vorliegende Arbeit besteht aus drei Teilprojekten. Das erste Projekt umfasst die Etablierung der nonsense-Suppression Methode zum Einbau von unnatürlichen Aminosäuren in Proteine. Dafür wurden die beiden Bausteine, das Dinukleotid pdCpa (5’-Phospho-2’-desoxycytidylyl(3’-5’)adenosin) und die verkürzte tRNA(-CA) mit dem amber Anticodon, zur Herstellung der vollständigen, misacylierten tRNA CUAuaa hergestellt. Das Dinukleotid konnte in sieben Stufen synthetisiert werden. Die verkürzte tRN...

  15. Detection and Quantification of CD4+ T Cells with Specificity for a New Major Histocompatibility Complex Class II-Restricted Influenza A Virus Matrix Protein Epitope in Peripheral Blood of Influenza Patients

    OpenAIRE

    Linnemann, Thomas; Jung, Günther; Walden, Peter

    2000-01-01

    FVFTLTVPS was identified as the core sequence of a new major histocompatibility complex class II-restricted T-cell epitope of influenza virus matrix protein. Epitope-specific CD4+ T cells were detected in the peripheral blood of patients with frequencies of up to 0.94%, depending on the number of additional terminal amino acids.

  16. Jacob Stael von Holstein ja palladionism eesti arhitektuuris / Ants Hein

    Index Scriptorium Estoniae

    Hein, Ants, 1952-

    2005-01-01

    Sõjaväeinsenerist ja arhitektist Jacob Stael von Holsteinist (1628, Pärnu-1679), tema kavandatud hoonetest ja rollist 17. sajandi II poole arhitektuuris. Fabian von Ferseni Toompeal Lossiplatsil asunud elamust (lammutati 1894) ja Maardu mõisahoonest, Hans von Ferseni Mäo mõisahoonest Järvamaal, Otto Wilhelm von Ferseni majast Toompeal, Axel von Roseni majast Tallinnas Pikk t. 28 (1670-74), Otto Reinhold von Taube majast Tartus (hävinud), J. S. von Holsteini enda hoonetest: majast Tallinnas Toompea 1, Anija mõisahoonest, elamust Riias jm. Bibliograafia lk. 452

  17. The effects of acute oral glutamine supplementation on exercise-induced gastrointestinal permeability and heat shock protein expression in peripheral blood mononuclear cells.

    Science.gov (United States)

    Zuhl, Micah; Dokladny, Karol; Mermier, Christine; Schneider, Suzanne; Salgado, Roy; Moseley, Pope

    2015-01-01

    Chronic glutamine supplementation reduces exercise-induced intestinal permeability and inhibits the NF-κB pro-inflammatory pathway in human peripheral blood mononuclear cells. These effects were correlated with activation of HSP70. The purpose of this paper is to test if an acute dose of oral glutamine prior to exercise reduces intestinal permeability along with activation of the heat shock response leading to inhibition of pro-inflammatory markers. Physically active subjects (N = 7) completed baseline and exercise intestinal permeability tests, determined by the percent ratio of urinary lactulose (5 g) to rhamnose (2 g). Exercise included two 60-min treadmill runs at 70 % of VO2max at 30 °C after ingestion of glutamine (Gln) or placebo (Pla). Plasma levels of endotoxin and TNF-α, along with peripheral blood mononuclear cell (PBMC) protein expression of HSP70 and IκBα, were measured pre- and post-exercise and 2 and 4 h post-exercise. Permeability increased in the Pla trial compared to that at rest (0.06 ± 0.01 vs. 0.02 ± 0.018) and did not increase in the Gln trial. Plasma endotoxin was lower at the 4-h time point in the Gln vs. 4 h in the Pla (6.715 ± 0.046 pg/ml vs. 7.952 ± 1.11 pg/ml). TNF-α was lower 4 h post-exercise in the Gln vs. Pla (1.64 ± 0.09 pg/ml vs. 1.87 ± 0.12 pg/ml). PBMC expression of IkBα was higher 4 h post-exercise in the Gln vs. 4 h in the Pla (1.29 ± 0.43 vs. 0.8892 ± 0.040). HSP70 was higher pre-exercise and 2 h post-exercise in the Gln vs. Pla (1.35 ± 0.21 vs. 1.000 ± 0.000 and 1.65 ± 0.21 vs. 1.27 ± 0.40). Acute oral glutamine supplementation prevents an exercise-induced rise in intestinal permeability and suppresses NF-κB activation in peripheral blood mononuclear cells. PMID:25062931

  18. Acute toxicity of phyto medicine Mulher Ativa and antioxidant properties on the labeling of blood cells and plasmatic proteins with 99mTc in vitro

    International Nuclear Information System (INIS)

    Full text: Medicinal plants originate natural products that are biologically active and widely employed as an alternative source in health care. Mulher Ativa is a phyto medicine used in several gynecological pathologies composed of eight medicinal plants which exhibits estrogen properties in the reproductive tract. The objective of this work was determining the acute toxicity studies investigated of Mulher Ativa (Ma) were performed in mice and antioxidant properties on the labeling of blood cells and plasmatic protein with 99mTc in vitro. For these studies, mice were divided in two groups, containing 05 animals each. The treated group received Ma in doses of 10, 100, 200, 300, 600, 1000, 2000, 3000 mg/kg of animal weight. Mice were carefully observed 0.5, 1, 2, 3, 4, 24, 48, and 72h after the treatment to assess possible clinical or toxicological symptoms. The second experiment was realized incubating heparin with blood carried out the experiments. Different concentrations of Mulher Ativa were chosen (200; 100; 50; 25; 12,5 mg/mL). A stannous chloride solution was also added and incubation was kept for 60 minutes. After this, 99mTc was added and the incubation was continued for 10 minutes. The mixture was centrifuged, precipitated with thichloroacetic acid 5% and soluble (SF) and insoluble fractions (IF) were separated. The radioactivities in the groups P, BC, IF-P, SF-P, IF- BC, SF-BC were determined in counter. The analysis of radioactivity in the samples of P and BC isolated from samples of whole blood treated with Mulher Ativa showed decrease significant (*p99mTc in the TCA-insoluble fraction of plasma. It is also concluded that presents antioxidant properties. As part of this pharmacological study, the acute toxicity of Ma in mice was first investigated. In these doses, the median lethal dose LD50 was determined to be higher than highest dose tested i.e 2.0 gkg -1 b.w. From this data, the estimated LD50 was 2060.1 mg/kg. The product was classified as slightly

  19. Non-immune binding of human IgG to M-related proteins confers resistance to phagocytosis of group A streptococci in blood.

    Directory of Open Access Journals (Sweden)

    Harry S Courtney

    Full Text Available The non-immune binding of immunoglobulins by bacteria is thought to contribute to the pathogenesis of infections. M-related proteins (Mrp are group A streptococcal (GAS receptors for immunoglobulins, but it is not known if this binding has any impact on virulence. To further investigate the binding of immunoglobulins to Mrp, we engineered mutants of an M type 4 strain of GAS by inactivating the genes for mrp, emm, enn, sof, and sfbX and tested these mutants in IgG-binding assays. Inactivation of mrp dramatically decreased the binding of human IgG, whereas inactivation of emm, enn, sof, and sfbx had only minor effects, indicating that Mrp is a major IgG-binding protein. Binding of human immunoglobulins to a purified, recombinant form of Mrp indicated that it selectively binds to the Fc domain of human IgG, but not IgA or IgM and that it preferentially bound subclasses IgG₁>IgG₄>IgG₂>IgG₃. Recombinant proteins encompassing different regions of Mrp were engineered and used to map its IgG-binding domain to its A-repeat region and a recombinant protein with 3 A-repeats was a better inhibitor of IgG binding than one with a single A-repeat. A GAS mutant expressing Mrp with an in-frame deletion of DNA encoding the A-repeats had a dramatically reduced ability to bind human IgG and to grow in human blood. Mrp exhibited host specificity in binding IgG; human IgG was the best inhibitor of the binding of IgG followed by pig, horse, monkey, and rabbit IgG. IgG from goat, mouse, rat, cow, donkey, chicken, and guinea pig were poor inhibitors of binding. These findings indicate that Mrp preferentially binds human IgG and that this binding contributes to the ability of GAS to resist phagocytosis and may be a factor in the restriction of GAS infections to the human host.

  20. Didaktik und Methodik von Präsenzveranstaltungen zur Vermittlung von Informationskompetenz an Hochschulbibliotheken: Grundlagen, Modelle, Perspektiven

    OpenAIRE

    Gran, Meike

    2009-01-01

    Die vorliegende Diplomarbeit vergleicht drei didaktisch-methodische Modelle von Präsenzveranstaltungen zur Vermittlung von Informationskompetenz an Hochschulbibliotheken. Neben den etablierten Konzepten Benutzerschulung und Teaching Library analysiert sie das von der Universitätsbibliothek Kassel entwickelte innovative Modell Learning Library. Die Arbeit untersucht jeweils die lerntheoretischen Grundlagen, das Verständnis von Informationskompetenz, Ziele und Inhalte, didaktische Prinzipien un...