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Sample records for blood lipid phenotypes

  1. A genome-wide association study for blood lipid phenotypes in the Framingham Heart Study

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    Abstract Background: Blood lipid levels including low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C), and triglycerides (TG) are highly heritable. Genome-wide association is a promising approach to map genetic loci related to these heritable phenotypes. Metho...

  2. Lipid profile of regular blood donors

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    Uche EI

    2013-05-01

    Full Text Available EI Uche,1 A Adediran,2 OD Damulak,3 TA Adeyemo,2 AA Akinbami,4 AS Akanmu21Department of Hematology and Blood Transfusion, Lagos University Teaching Hospital, Lagos, Nigeria; 2Department of Hematology and Blood Transfusion, University of Lagos, Lagos, Nigeria; 3Department of Hematology and Blood Transfusion, Jos University Teaching Hospital, Jos, Nigeria; 4Department of Hematology and Blood Transfusion, Lagos State University, Ikeja, NigeriaIntroduction: A few reports have linked regular blood donation to the lowering of parameters of lipid profile. Estimating the lipid profile is an accepted method of assessing an individual’s risk for coronary heart disease, particularly if there is evidence of lipid peroxidation. Regular blood donation may lower iron stores, and this in turn lowers lipid peroxidation. This study was carried out to determine the effect of blood donation on lipid profile.Materials and methods: Eighty-two participants consented to participate and were enrolled into the study, 52 of whom were regular blood donors (study group and 30 were non-donors (control group. Venous blood (10 mL was drawn from each subject into new plain screw-capped disposable plastic tubes. This was allowed to clot and the serum was used to determine total cholesterol, triglycerides, low-density lipoprotein, and high-density lipoprotein.Results: The mean total cholesterol (4.66 ± 0.86 mmol/L, triglycerides (1.22 ± 0.64 mmol/L, and low-density lipoprotein (2.32 ± 0.73 mmol/L were significantly lower in the regular blood donors than the control group (5.61 ± 1.26 mmol/L, 1.77 ± 2.9 mmol/L, and 3.06 ± 0.89 mmol/L, respectively; P < 0.05 in all cases. Also, while 42% of the study group had a low/high-density lipoprotein ratio of at least three, about 57% of the control group had a ratio of at least three (P = 0.21.Conclusion: Regular blood donation may be protective against cardiovascular disease as reflected by significantly lower mean total

  3. Medulloblastoma Genotype Dictates Blood Brain Barrier Phenotype.

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    Phoenix, Timothy N; Patmore, Deanna M; Boop, Scott; Boulos, Nidal; Jacus, Megan O; Patel, Yogesh T; Roussel, Martine F; Finkelstein, David; Goumnerova, Liliana; Perreault, Sebastien; Wadhwa, Elizabeth; Cho, Yoon-Jae; Stewart, Clinton F; Gilbertson, Richard J

    2016-04-11

    The childhood brain tumor, medulloblastoma, includes four subtypes with very different prognoses. Here, we show that paracrine signals driven by mutant β-catenin in WNT-medulloblastoma, an essentially curable form of the disease, induce an aberrant fenestrated vasculature that permits the accumulation of high levels of intra-tumoral chemotherapy and a robust therapeutic response. In contrast, SHH-medulloblastoma, a less curable disease subtype, contains an intact blood brain barrier, rendering this tumor impermeable and resistant to chemotherapy. The medulloblastoma-endothelial cell paracrine axis can be manipulated in vivo, altering chemotherapy permeability and clinical response. Thus, medulloblastoma genotype dictates tumor vessel phenotype, explaining in part the disparate prognoses among medulloblastoma subtypes and suggesting an approach to enhance the chemoresponsiveness of other brain tumors. PMID:27050100

  4. Partial phenotyping in voluntary blood donors of Gujarat State

    OpenAIRE

    Maitrey Gajjar; Tarak Patel; Nidhi Bhatnagar; Kruti Patel; Mamta Shah; Amit Prajapati

    2016-01-01

    Introduction: Partial phenotyping of voluntary blood donors has vital role in transfusion practice, population genetic study and in resolving legal issues. The Rh blood group is one of the most complex and highly immunogenic blood group known in humans. The Kell system, discovered in 1946, is the third most potent system at triggering hemolytic transfusion reactions and consists of 25 highly immunogenic antigens. Knowledge of Rh & Kell phenotypes in given population is relevant for better pla...

  5. The phenotypic spectrum of neutral lipid storage myopathy due to mutations in the PNPLA2 gene.

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    Reilich, Peter; Horvath, Rita; Krause, Sabine; Schramm, Nicolai; Turnbull, Doug M; Trenell, Michael; Hollingsworth, Kieren G; Gorman, Grainne S; Hans, Volkmar H; Reimann, Jens; MacMillan, Andrée; Turner, Lesley; Schollen, Annette; Witte, Gregor; Czermin, Birgit; Holinski-Feder, Elke; Walter, Maggie C; Schoser, Benedikt; Lochmüller, Hanns

    2011-11-01

    Neutral lipid storage disease is caused by mutations in the CGI-58 or the PNPLA2 genes. Lipid storage can be detected in various cell types including blood granulocytes. While CGI-58 mutations are associated with Chanarin-Dorfman syndrome, a condition characterized by lipid storage and skin involvement (ichthyosis), mutations in the patatin-like phospholipase domain-containing protein 2 gene (PNPLA2) were reported with skeletal and cardiac muscle disease only. We describe clinical, myopathological, magnetic resonance imaging (MRI), and genetic findings of six patients carrying different recessive PNPLA2 mutations. Pulse-chase labeling of control and patient cells with supplementation of clenbuterol, salmeterol, and dexamethasone was performed in vitro. The patients share a recognizable phenotype with prominent shoulder girdle weakness and mild pelvic girdle and distal muscle weakness, with highly elevated creatine kinase (CK) and cardiomyopathy developing at later stages. Muscle histology invariably reveals massive accumulation of lipid droplets. New muscle or whole-body MRI techniques may assist diagnosis and may become a useful tool to quantify intramuscular lipid storage. Four novel and two previously reported mutations were detected, affecting different parts of the PNPLA2 gene. Activation of hormone-sensitive lipase by beta-adrenergic substances such as clenbuterol appears to bypass the enzymatic block in PNPLA2-deficient patient cells in vitro. PNPLA2 deficiency is a slowly progressive myopathy with onset around the third decade. Cardiac involvement is relatively common at a later stage. Muscle MRI may detect increased lipid in a characteristic distribution, which could be used for monitoring disease progression. Beta-adrenergic agents may be beneficial in improving triacylglycerol breakdown in patients with PNPLA2 mutations. PMID:21544567

  6. Influence of Blood Lipids on Global Coagulation Test Results

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    Kim, Jung-Ah; Kim, Ji-Eun; Song, Sang Hoon; Kim, Hyun Kyung

    2014-01-01

    Background High levels of blood lipids have been associated with high levels of coagulation factors. We investigated whether blood lipids influence the results of global coagulation tests, including prothrombin time (PT), activated partial thromboplastin time (aPTT), and thrombin generation assay (TGA). Methods PT, aPTT, and TGA, along with procoagulant and anticoagulant factors, were measured in 488 normal individuals. Vitamin K status was assessed with prothrombin-induced by vitamin K absen...

  7. Partial phenotyping in voluntary blood donors of Gujarat State

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    Maitrey Gajjar

    2016-01-01

    Full Text Available Introduction: Partial phenotyping of voluntary blood donors has vital role in transfusion practice, population genetic study and in resolving legal issues.The Rh blood group is one of the most complex and highly immunogenic blood group known in humans. The Kell system, discovered in 1946, is the third most potent system at triggering hemolytic transfusion reactions and consists of 25 highly immunogenic antigens. Knowledge of Rh & Kell phenotypes in given population is relevant for better planning and management of blood bank; the main goal is to find compatible blood for patients needing multiple blood transfusions. The aim of this study was to evaluate the frequency of Rh & Kell phenotype of voluntary donors in Gujarat state. Materials and Methods: The present study was conducted by taking 5670 samples from random voluntary blood donors coming in blood donation camp. Written consent was taken for donor phenotyping. The antigen typing of donors was performed by Qwalys-3(manufacturer: Diagast by using electromagnetic technology on Duolys plates. Results: Out of 5670 donors, the most common Rh antigen observed in the study population was e (99.07% followed by D (95.40%, C (88.77%, c (55.89% and E (17.88%. The frequency of the Kell antigen (K was 1.78 %. Discussion: The antigen frequencies among blood donors from Gujarat were compared with those published for other Indian populations. The frequency of D antigen in our study (95.4% and north Indian donors (93.6 was significantly higher than in the Caucasians (85% and lower than in the Chinese (99%. The frequencies of C, c and E antigens were dissimilar to other ethnic groups while the ′e′ antigen was present in high frequency in our study as also in the other ethnic groups. Kell antigen (K was found in only 101 (1.78 % donors out of 5670. Frequency of Kell antigen in Caucasian and Black populations is 9% & 2% respectively. The most common Kell phenotype was K-k+, not just in Indians (96.5% but

  8. Phenotype and Functions of Memory Tfh cells in Human Blood

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    Schmitt, Nathalie; Bentebibel, Salah-Eddine; Ueno, Hideki

    2014-01-01

    Our understanding of the origin and functions of human blood CXCR5+ CD4+ T cells found in human blood has changed dramatically in the past years. These cells are currently considered to represent a circulating memory compartment of T follicular helper (Tfh)-lineage cells. Recent studies have shown that blood memory Tfh cells are composed of phenotypically and functionally distinct subsets. Here we review the current understanding of human blood memory Tfh cells and the subsets within this compartment. We present a strategy to define these subsets based on cell surface profiles. Finally, we discuss how increased understanding of the biology of blood memory Tfh cells may contribute insight into the pathogenesis of autoimmune diseases and the mode of action of vaccines. PMID:24998903

  9. Correlation between Duffy blood group phenotype and breast cancer incidence

    International Nuclear Information System (INIS)

    Different ethnicities have different distribution of Duffy blood group (DBG) phenotypes and different breast cancer morbidity. A study in our lab demonstrated that Duffy antigen/receptor for chemokines (DARC, also known as DBGP, the Duffy protein phenotype), led to the inhibition of tumorigenesis. Therefore, we tested the hypothesis that DBGP is correlated with breast cancer occurrence. DBGP proteins were examined by indirect antiglobulin testing with anti-FYa and anti-FYb antibodies. The phenotypes were classified into four groups according to the agglutination reactions: FYa + FYb+, FYa + FYb-, FYa-FYb + and FYa-FYb-. The phenotypes and pathological diagnosis of consecutively hospitalized female patients (n = 5,022) suffering from breast cancer at the Shanghai Cancer Hospital and Henan Province Cancer Hospital were investigated. The relationships between DBGP expression with breast cancer occurrence, axillary lymph status, histological subtype, tumor size pathological grade and overall survival were analyzed. The incidence of breast cancer was significantly different between FYa + FYb + (29.8%), FYa + FYb- (33.2%), FYa-FYb + (45.6%) and FYa-FYb- (59.1%; P = 0.001). Significant different numbers of breast cancer patients had metastases to the axillary lymph nodes in the FYa + FYb + group (25.1%), FYa + FYb- (36.9%), FYa-FYb + (41.0%) and FYa-FYb- (50.0%, (P = 0.005). There was a statistical significance (p = 0.022) of the overall survival difference between patients with difference phenotypes. No significant difference was observed in cancer size (t-test, p > 0.05), histological cancer type (Fisher's exact test, p > 0.05) or histological grade (Fisher's exact test, p > 0.05) between every each DBGP group. DBGP is correlated with breast cancer incidence and axillary lymph node metastasis and overall survival. Further investigations are required to determine the underlying mechanism of Duffy blood group phenotype on breast cancer risk

  10. Correlation between Duffy blood group phenotype and breast cancer incidence

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    Liu Xiao-feng

    2012-08-01

    Full Text Available Abstract Background Different ethnicities have different distribution of Duffy blood group (DBG phenotypes and different breast cancer morbidity. A study in our lab demonstrated that Duffy antigen/receptor for chemokines (DARC, also known as DBGP, the Duffy protein phenotype, led to the inhibition of tumorigenesis. Therefore, we tested the hypothesis that DBGP is correlated with breast cancer occurrence. Methods DBGP proteins were examined by indirect antiglobulin testing with anti-FYa and anti-FYb antibodies. The phenotypes were classified into four groups according to the agglutination reactions: FYa + FYb+, FYa + FYb-, FYa-FYb + and FYa-FYb-. The phenotypes and pathological diagnosis of consecutively hospitalized female patients (n = 5,022 suffering from breast cancer at the Shanghai Cancer Hospital and Henan Province Cancer Hospital were investigated. The relationships between DBGP expression with breast cancer occurrence, axillary lymph status, histological subtype, tumor size pathological grade and overall survival were analyzed. Results The incidence of breast cancer was significantly different between FYa + FYb + (29.8%, FYa + FYb- (33.2%, FYa-FYb + (45.6% and FYa-FYb- (59.1%; P = 0.001. Significant different numbers of breast cancer patients had metastases to the axillary lymph nodes in the FYa + FYb + group (25.1%, FYa + FYb- (36.9%, FYa-FYb + (41.0% and FYa-FYb- (50.0%, (P = 0.005. There was a statistical significance (p = 0.022 of the overall survival difference between patients with difference phenotypes. No significant difference was observed in cancer size (t-test, p > 0.05, histological cancer type (Fisher's exact test, p > 0.05 or histological grade (Fisher's exact test, p > 0.05 between every each DBGP group. Conclusions DBGP is correlated with breast cancer incidence and axillary lymph node metastasis and overall survival. Further investigations are required to determine the underlying mechanism of Duffy blood group phenotype

  11. Para-Bombay phenotype: report of a rare blood group

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    A. Yashovardhan

    2012-07-01

    Full Text Available The blood sample of a 54-year-old male patient who presented with signs and symptoms suggestive of anaemia was submitted to the Blood Bank for blood grouping and cross-matching. In forward grouping, no agglutination was observed with A, B and AB antisera, but agglutination was noticed with D antiserum (Group O. In reverse grouping, there was agglutination in tube labelled A and no agglutination in tubes B and O (Group B resulting in discrepancy between forward and reverse grouping. Further testing confirmed that the individual's blood group was Para-Bombay B (Para-BH, which is a rare entity. The Para-Bombay phenotype is very rare. Only a few cases of Para-Bombay were reported in India till now and none from Andhra Pradesh. This entity is characterized by the absence of H, A and B antigens on the red cells but their presence in saliva and secretions of gastrointestinal and genitourinary tracts. Proper identification of this phenotype is very important; otherwise this particular blood group may be mislabelled as group O.

  12. Aluminum induces lipid peroxidation and aggregation of human blood platelets

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    Neiva T.J.C.

    1997-01-01

    Full Text Available Aluminum (Al3+ intoxication is thought to play a major role in the development of Alzheimer's disease and in certain pathologic manifestations arising from long-term hemodialysis. Although the metal does not present redox capacity, it can stimulate tissue lipid peroxidation in animal models. Furthermore, in vitro studies have revealed that the fluoroaluminate complex induces diacylglycerol formation, 43-kDa protein phosphorylation and aggregation. Based on these observations, we postulated that Al3+-induced blood platelet aggregation was mediated by lipid peroxidation. Using chemiluminescence (CL of luminol as an index of total lipid peroxidation capacity, we established a correlation between lipid peroxidation capacity and platelet aggregation. Al3+ (20-100 µM stimulated CL production by human blood platelets as well as their aggregation. Incubation of the platelets with the antioxidants nor-dihydroguaiaretic acid (NDGA (100 µM and n-propyl gallate (NPG (100 µM, inhibitors of the lipoxygenase pathway, completely prevented CL and platelet aggregation. Acetyl salicylic acid (ASA (100 µM, an inhibitor of the cyclooxygenase pathway, was a weaker inhibitor of both events. These findings suggest that Al3+ stimulates lipid peroxidation and the lipoxygenase pathway in human blood platelets thereby causing their aggregation

  13. Blood cadmium concentration and lipid profile in Korean adults

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    Kim, Kisok, E-mail: kimkisok@kmu.ac.kr [Department of Public Health, Keimyung University, 1000 Shindang-dong, Daegu 704-701 (Korea, Republic of)

    2012-01-15

    Although animal experiments have shown that cadmium exposure induces alterations in lipid profiles, no epidemiological study of this relationship has been performed. The objective of this study was to evaluate the association between blood cadmium concentration and blood lipid levels in Korean adults. A cross-sectional study comprising participants (n=3903) aged 20 years or older from the 2005, 2008, and 2009 Korea National Health and Nutrition Examination Surveys was conducted. Demographic characteristics and dietary intake were obtained from the participants by questionnaire, and cadmium and lipid levels were determined by analysis of blood samples. After adjusting for demographic and dietary factors, blood concentration of cadmium was positively associated with the risk of low high-density lipoprotein cholesterol (HDL-C) in a dose-dependent manner (p for trend <0.001). In addition, the odds ratios (ORs) of a high triglyceride to HDL-C ratio was significantly increased in the high blood cadmium groups [OR=1.36; 95% confidence interval (CI), 1.03-1.79 for fourth quintile and OR=1.41; 95% CI, 1.07-1.86 for fifth quintile] compared with the lowest quintile group. However, high blood cadmium was not associated with a risk of high total cholesterol, high low-density lipoprotein cholesterol, or high triglycerides. These data suggest that an increased cadmium body burden increases the risk of dyslipidemia, mainly due to the increased risk of low HDL-C and the high ratio of triglycerides to HDL-C.

  14. Lipid-laden cells differentially distributed in the aging brain are functionally active and correspond to distinct phenotypes.

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    Shimabukuro, Marilia Kimie; Langhi, Larissa Gutman Paranhos; Cordeiro, Ingrid; Brito, José M; Batista, Claudia Maria de Castro; Mattson, Mark P; Mello Coelho, Valeria de

    2016-01-01

    We characterized cerebral Oil Red O-positive lipid-laden cells (LLC) of aging mice evaluating their distribution, morphology, density, functional activities and inflammatory phenotype. We identified LLC in meningeal, cortical and neurogenic brain regions. The density of cerebral LLC increased with age. LLC presenting small lipid droplets were visualized adjacent to blood vessels or deeper in the brain cortical and striatal parenchyma of aging mice. LLC with larger droplets were asymmetrically distributed in the cerebral ventricle walls, mainly located in the lateral wall. We also found that LLC in the subventricular region co-expressed beclin-1 or LC3, markers for autophagosome or autophagolysosome formation, and perilipin (PLIN), a lipid droplet-associated protein, suggesting lipophagic activity. Some cerebral LLC exhibited β galactosidase activity indicating a senescence phenotype. Moreover, we detected production of the pro-inflammatory cytokine TNF-α in cortical PLIN(+) LLC. Some cortical NeuN(+) neurons, GFAP(+) glia limitans astrocytes, Iba-1(+) microglia and S100β(+) ependymal cells expressed PLIN in the aging brain. Our findings suggest that cerebral LLC exhibit distinct cellular phenotypes and may participate in the age-associated neuroinflammatory processes. PMID:27029648

  15. Blood lipid levels and prostate cancer risk; a cohort study

    NARCIS (Netherlands)

    Kok, D.E.G.; Roermund, van J.G.H.; Aben, K.K.H.; Heijer, den M.; Swinkels, D.W.; Kampman, E.; Kiemeney, L.A.L.M.

    2011-01-01

    It has been hypothesized that blood lipid levels might be associated with prostate cancer risk. The aim of the present study was to evaluate the association between serum total cholesterol, high-density lipoprotein (HDL) cholesterol, low-density lipoprotein (LDL) cholesterol, triglycerides and prost

  16. Alcohol consumption and blood lipids in elderly coronary patients

    NARCIS (Netherlands)

    Jong, de H.J.I.; Goede, de J.; Oude Griep, L.M.; Geleijnse, J.M.

    2008-01-01

    Alcohol may have a beneficial effect on coronary heart disease (CHD) that could be mediated by elevation of high-density lipoprotein cholesterol (HDLC). Data on alcohol consumption and blood lipids in coronary patients are scarce. We studied whether total ethanol intake and consumption of specific t

  17. Estimated daily salt intake in relation to blood pressure and blood lipids

    DEFF Research Database (Denmark)

    Thuesen, Betina H; Toft, Ulla; Buhelt, Lone P;

    2015-01-01

    BACKGROUND: Excessive salt intake causes increased blood pressure which is considered the leading risk for premature death. One major challenge when evaluating associations between daily salt intake and markers of non-communicable diseases is that a high daily salt intake correlates with obesity......, which is also a well described risk factor for poor cardiometabolic outcome. The aim of this study was to evaluate the relationship of estimated daily salt intake with blood pressure and blood lipids and to investigate the effect of taking different measures of obesity into account. METHODS: We included...... estimated 24-hour sodium excretion with blood pressure and blood lipids were evaluated by linear regression models. RESULTS: The daily mean estimated intake of salt was 10.80 g and 7.52 g among men and women, respectively. Daily salt intake was significantly associated with blood pressure (β-estimates 1...

  18. Blood cadmium concentration and lipid profile in Korean adults

    International Nuclear Information System (INIS)

    Although animal experiments have shown that cadmium exposure induces alterations in lipid profiles, no epidemiological study of this relationship has been performed. The objective of this study was to evaluate the association between blood cadmium concentration and blood lipid levels in Korean adults. A cross-sectional study comprising participants (n=3903) aged 20 years or older from the 2005, 2008, and 2009 Korea National Health and Nutrition Examination Surveys was conducted. Demographic characteristics and dietary intake were obtained from the participants by questionnaire, and cadmium and lipid levels were determined by analysis of blood samples. After adjusting for demographic and dietary factors, blood concentration of cadmium was positively associated with the risk of low high-density lipoprotein cholesterol (HDL-C) in a dose-dependent manner (p for trend <0.001). In addition, the odds ratios (ORs) of a high triglyceride to HDL-C ratio was significantly increased in the high blood cadmium groups [OR=1.36; 95% confidence interval (CI), 1.03–1.79 for fourth quintile and OR=1.41; 95% CI, 1.07–1.86 for fifth quintile] compared with the lowest quintile group. However, high blood cadmium was not associated with a risk of high total cholesterol, high low-density lipoprotein cholesterol, or high triglycerides. These data suggest that an increased cadmium body burden increases the risk of dyslipidemia, mainly due to the increased risk of low HDL-C and the high ratio of triglycerides to HDL-C.

  19. Blood Lipids and other Related Risk Factors of stroke

    Institute of Scientific and Technical Information of China (English)

    Liu YAND; Hiu Lu

    2000-01-01

    Abstract To assess the ralationship between incidence of stroke and blood lipids, apolipoprolcin (apo) , 219 patients with stroke(cerebral hemorrhage 87, cerebral infarction 132) , diagnosed with the aid of CT, were examined. The levels of serum total cholesterol, triglycerioes, apo-A, apo-B were calculatea. 160 cases with no disorder in metabolism of blood lipids or other disease of meurologic system were taken as centrol group. The results zhowed that the ratio of high density lipoprotein cholesterol and low clensity lipoprotein cholesterol was iower than.that of control group(p<0. 05) . In the patients group, cases with diabetes, hypentension coronary heart disease or onesity, the levels of blood liprds. triglyceride and cholesterol were higher than those vithout above mentioned disease The condusion is, that there is certain reletionship between the contents of bliid lipids. lipoprotein and it sulqraction HDL2, HDL3 and cerebrovasculor cisease. The radis of high density lipoprotein and low density lipoprolein is the important factor in predicting of stroke

  20. Effect of Kombucha Tea on Blood Sugar and Blood Lipid Profiles in Diabetic Rats: Short Communication

    OpenAIRE

    Mohammad Malekaneh; Sara Sadeghi Zadeh Bafandeh; Fatemeh Hajipoor; Mohsen Naseri

    2015-01-01

    Background and Aim: Kombucha tea is made through fermentation of sweet tea by Kombucha fungus. It has beneficial therapeutic effects in the treatment of many diseases. The present study aimed at examining the effect of Kombucha tea extract on blood sugar and blood lipid profiles in diabetes. Materials and Methods: Twenty-eight Wistar rats weighing about 250g were divided into 4 equal groups and then injected intraperitoneally (IP) with alloxan to induce diabetes. The administered concentratio...

  1. Fatty acid binding protein 3 (fabp3) is associated with insulin, lipids and cardiovascular phenotypes of the metabolic syndrome through epigenetic modifications in a northern european family population

    OpenAIRE

    Zhang, Yi; Kent, Jack W; Lee, Adam; Cerjak, Diana; ALI Omar; Diasio, Robert; Olivier, Michael; Blangero, John; Carless, Melanie A.; Kissebah, Ahmed H.

    2013-01-01

    Background Fatty acid-binding proteins (FABPs) play regulatory roles at the nexus of lipid metabolism and signaling. Dyslipidemia in clinical manifestation frequently co-occurs with obesity, insulin resistance and hypertension in the Metabolic Syndrome (MetS). Animal studies have suggested FABPs play regulatory roles in expressing MetS phenotypes. In our family cohort of Northern European descent, transcript levels in peripheral white blood cells (PWBCs) of a key FABPs, FABP3, is correlated w...

  2. Fat induced hypertension in rabbits. Effects of dietary fibre on blood pressure and blood lipid concentration.

    Science.gov (United States)

    Burstyn, P G; Husbands, D R

    1980-04-01

    Rabbits were fed diets containing 200 g.kg-1 coconut oil, palm oil, or safflower oil. Some of the diets also contained 200 g.kg-1 cellulose. The blood pressure was measured daily by a non-invasive technique for the 2 month duration of the experiment. Blood samples were drawn after an overnight fast at intervals during the experiment and analysed for lipids. Blood pressure was always increased by a fat-enriched diet. This effect was diminished and delayed by adding cellulose to the diets, though cellulose itself had no effect on the blood pressure in the absence of fat. There was a modest negative correlation between fasting serum triglyceride concentration and the blood pressure in animals fed fat enriched diets without added cellulose, but not in animals fed diets containing both fat and cellulose. These results coupled with those of Wright, Burstyn and Gibney may serve partly to explain the observation that vegetarians have lower blood pressures than omnivores, the latter consuming diets which are relatively richer in fats and poorer in fibre than the former. PMID:6253068

  3. Lessons and Implications from Genome-Wide Association Studies (GWAS Findings of Blood Cell Phenotypes

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    Nathalie Chami

    2014-01-01

    Full Text Available Genome-wide association studies (GWAS have identified reproducible genetic associations with hundreds of human diseases and traits. The vast majority of these associated single nucleotide polymorphisms (SNPs are non-coding, highlighting the challenge in moving from genetic findings to mechanistic and functional insights. Nevertheless, large-scale (epigenomic studies and bioinformatic analyses strongly suggest that GWAS hits are not randomly distributed in the genome but rather pinpoint specific biological pathways important for disease development or phenotypic variation. In this review, we focus on GWAS discoveries for the three main blood cell types: red blood cells, white blood cells and platelets. We summarize the knowledge gained from GWAS of these phenotypes and discuss their possible clinical implications for common (e.g., anemia and rare (e.g., myeloproliferative neoplasms human blood-related diseases. Finally, we argue that blood phenotypes are ideal to study the genetics of complex human traits because they are fully amenable to experimental testing.

  4. Quantitative trait loci influencing cholesterol and phospholipid phenotypes map to chromosomes that contain genes regulating blood pressure in the spontaneously hypertensive rat.

    OpenAIRE

    Bottger, A.; van Lith, H.A.; Kren, V.; Krenová, D; Bílá, V; Vorlícek, J; Zídek, V; Musilová, A; Zdobinská, M; J. M. Wang; van Zutphen, B F; Kurtz, T. W.; Pravenec, M.

    1996-01-01

    The frequent coincidence of hypertension and dyslipidemia suggests that related genetic factors might underlie these common risk factors for cardiovascular disease. To investigate whether quantitative trait loci (QTLs) regulating lipid levels map to chromosomes known to contain genes regulating blood pressure, we used a genome scanning approach to map QTLs influencing cholesterol and phospholipid phenotypes in a large set of recombinant inbred strains and in congenic strains derived from the ...

  5. The Mixture of Yogurt and Strawberry Juice to Repair Blood Lipid Profile

    OpenAIRE

    Rince Alfia Fadri; Rilma Novita; Fadil Oenzil; Kesuma Sayuti

    2014-01-01

    There are so many ways to do in reducing cholesterol concentration in blood. Yogurt and strawberry juice have ability  to repair blood lipid profile at hypercholesterolemia. Research and lactobacillus development potention as the cholesterol killing probiotic that to be mixed with strawberry juice is necessary to do to support community health. The purpose of this reasearch was measuring the mixture of yogurt and strawberry juice effectiveness to repair blood lipid profile.  The design of thi...

  6. In Situ Characterizing Membrane Lipid Phenotype of Human Lung Cancer Cell Lines Using Mass Spectrometry Profiling

    Science.gov (United States)

    He, Manwen; Guo, Shuai; Ren, Junling; Li, Zhili

    2016-01-01

    Abnormal lipid metabolisms are closely associated with cancers. In this study, mass spectrometry was employed to in situ investigate the associations of membrane lipid phenotypes of six human lung cancer cell lines (i.e., A549, H1650, H1975 from adenocarcinoma, H157 and H1703 from squamous cell carcinomas, and H460 from a large cell carcinoma) with cancer cell types and finally total 230 lipids were detected. Based these 230 lipids, partial least-square discriminant analysis indicated that fifteen lipids (i.e., PE 18:0_18:1, PI 18:0_20:4, SM 42:2, PE 16:0_20:4, PE 36:2, PC 36:2, SM 34:1, PA 38:3,C18:0, C22:4, PA 34:2, C20:5, C20:2, C18:2, and CerP 36:2) with variable importance in the projection (VIP) value of > 1.0 could be used to differentiate six cancer cell lines with the Predicted Residual Sum of Square (PRESS) score of 0.1974. Positive correlation between polyunsaturated fatty acids (i.e., C20:4, C22:4, C22:5, and C22:6) and polyunsaturated phospholipids (PE 16:0_20:4, PE 38:4, and PI 18:0_20:4) was observed in lung adenocarcinoma cells, especially for H1975 cells. Three adenocarcinoma cell lines (i.e., A549, H1650, and H1975) could be differentiated from other lung cancer cell lines based on the expression of C18:1, C20:1, C20:2, C20:5, and C22:6.

  7. Lipid accumulation product (LAP) as a criterion for the identification of the healthy obesity phenotype in postmenopausal women.

    Science.gov (United States)

    Lwow, Felicja; Jedrzejuk, Diana; Milewicz, Andrzej; Szmigiero, Leszek

    2016-09-01

    Obesity and its complications constitute a major health problem in postmenopausal women. The identification of the obesity phenotype, especially that of metabolically healthy obese (MHO) patients, is a necessary part of obesity treatment protocols. There are several methods to define MHO, but unfortunately, all of them are arbitrary and inconsistent. The aim of this work was to determine whether lipid accumulation product (LAP) could be used as a marker of the MHO phenotype in postmenopausal women. A sample of 345 Polish postmenopausal women aged 50-60years old participated in the study. Participants were classified as obese when their BMI was >27. Receiver operating characteristic curve analysis was performed to estimate the best cutoff for the LAP index value to identify postmenopausal women without metabolic syndrome components. We found that the best cutoff value was LAP ≤29.9, and this value was used to define MHO individuals. With this definition, the identification of MHO individuals could be made when both of the following criteria were met: LAP index ≤29.9 and no arterial hypertension (SBP<130mmHg, DBP<85mmHg). The anthropometric and body fat distribution measurements, as well as the metabolic characteristics of MHO women identified according to the above definition, were compared with those of MHO women identified by two other methods in the literature. These methods and our definition identified similar proportions of MHO women ranging from 11.6% to 16.9%. We found that MHO women identified by all of the definitions used in this study possessed a similar metabolic status, and they did not differ in anthropometric indices or body fat distribution measurements. We concluded that the combination of LAP estimation and arterial blood pressure measurement appear to constitute a useful method for identifying the MHO phenotype in postmenopausal women. PMID:27329927

  8. Para-Bombay phenotype: report of a rare blood group

    OpenAIRE

    A.Yashovardhan; I.S.Chaitanya Kumar; K.V. Sreedhar Babu; B. Suresh Babu; Anju Verma; Jothi Bai, D.S.; B. Siddhartha Kumar

    2012-01-01

    The blood sample of a 54-year-old male patient who presented with signs and symptoms suggestive of anaemia was submitted to the Blood Bank for blood grouping and cross-matching. In forward grouping, no agglutination was observed with A, B and AB antisera, but agglutination was noticed with D antiserum (Group O). In reverse grouping, there was agglutination in tube labelled A and no agglutination in tubes B and O (Group B) resulting in discrepancy between forward and reverse grouping. Furth...

  9. Mortality and cancer in relation to ABO blood group phenotypes in the Golestan Cohort Study

    OpenAIRE

    Etemadi, Arash; Kamangar, Farin; Islami, Farhad; Poustchi, Hossein; Pourshams, Akram; Brennan, Paul; Boffetta, Paolo; Malekzadeh, Reza; Dawsey, Sanford M.; Abnet, Christian C.; Emadi, Ashkan

    2015-01-01

    Background A few studies have shown an association between blood group alleles and vascular disease, including atherosclerosis, which is thought to be due to the higher level of von Willebrand factor in these individuals and the association of blood group locus variants with plasma lipid levels. No large population-based study has explored this association with overall and cause-specific mortality. Methods We aimed to study the association between ABO blood groups and overall and cause-specif...

  10. Veganism Is a Viable Alternative to Conventional Diet Therapy for Improving Blood Lipids and Glycemic Control.

    Science.gov (United States)

    Trepanowski, John F; Varady, Krista A

    2015-01-01

    The American Diabetes Association (ADA) and the National Cholesterol Education Program (NCEP) have each outlined a set of dietary recommendations aimed at improving glycemic control and blood lipids, respectively. However, traditional vegan diets (low-fat diets that proscribe animal product consumption) are also effective at improving glycemic control, and dietary portfolios (vegan diets that contain prescribed amounts of plant sterols, viscous fibers, soy protein, and nuts) are also effective at improving blood lipids. The purpose of this review was to compare the effects of traditional vegan diets and dietary portfolios with ADA and NCEP diets on body weight, blood lipids, blood pressure, and glycemic control. The main findings are that traditional vegan diets appear to improve glycemic control better than ADA diets in individuals with type 2 diabetes mellitus (T2DM), while dietary portfolios have been consistently shown to improve blood lipids better than NCEP diets in hypercholesterolemic individuals. PMID:24922183

  11. Comparison of Blood Lipids, Blood Pressures and Left Ventricular Cavity Dimension between Soccer Players and Non-Athletes

    Science.gov (United States)

    Gokhan, Ismail; Kurkcu, Recep; Cekin, Resul

    2013-01-01

    In this study, it was aimed to compare the investigate the effects of regular exercise on blood lipids, blood pressure and left ventricular cavity dimensions function between soccer players and non-athletes in football players. This study consisted included a total of 30 subjects, including an experimental group including 18 soccer players…

  12. Characteristic DNA methylation profiles in peripheral blood monocytes are associated with inflammatory phenotypes of asthma

    OpenAIRE

    Gunawardhana, Lakshitha P; Gibson, Peter G; Simpson, Jodie L.; Benton, Miles C.; Rodney A. Lea; Baines, Katherine J

    2014-01-01

    Epigenetic changes including DNA methylation caused by environmental exposures may contribute to the heterogeneous inflammatory response in asthma. Here we investigate alterations in DNA methylation of purified blood monocytes that are associated with inflammatory phenotypes of asthma. Peripheral blood was collected from adults with eosinophilic asthma (EA; n = 21), paucigranulocytic asthma (PGA; n = 22), neutrophilic asthma (NA; n = 9), and healthy controls (n = 10). Blood monocytes were iso...

  13. No Association of Phenotypic ABO Blood Group and Malaria during Pregnancy

    OpenAIRE

    Boel, Machteld E.; Marcus J Rijken; Pimanpanarak, Mupawjay; Keereecharoen, Naw Lily; Proux, Stephane; Nosten, François; McGready, Rose

    2012-01-01

    In a few small studies an association between blood group O and placental malaria has been described. The relationship between blood group and malaria in pregnancy (Plasmodium vivax and Plasmodium falciparum) was analyzed in 1,468 women from three longitudinal cohort studies in which weekly malaria screening was done systematically during pregnancy. One-third of women (447 of 1,468) had at least one malaria infection in pregnancy. The ABO blood group phenotype was not associated with the spec...

  14. Detection of rare blood group, Bombay (Oh phenotype patients and management by acute normovolemic hemodilution

    Directory of Open Access Journals (Sweden)

    Manisha Shrivastava

    2015-01-01

    Full Text Available Background: Due to lack of correct blood grouping practices, the rare Bombay Oh phenotype may be missed, subjecting patients to the risk of severe hemolytic transfusion reaction. In the absence of blood donor registry, transfusion management of patients needing immediate surgery is a challenge. This study presents detection of rare Bombay Oh phenotype patients and their management by acute peri-operative acute normovolemic hemodilution (ANH in a hospital from central India. Materials and Methods: Blood grouping of patients and blood donors with a standard tube method was carried out and samples identified as rare Bombay phenotype were confirmed by saliva inhibition test. Surgical management of cases needing transfusion was done by ANH, as per the British Committee for Standards in Hematology guidelines. Results: The incidence of Bombay phenotype was 0.002% or 1 in 51,924 in the study. Amongst three cases (patients identified as Bombay phenotype, one was Bombay Oh, Rh negative. Two cases were missed in the first instance and one case actually did not require transfusion. In the absence of a blood donor registry for Bombay phenotype, the cases needing transfusion were successfully managed with ANH in the operation theatre. Conclusion: A simple test like blood grouping should be done with serious intention with incorporation of both forward and reverse grouping, so that no patient receives wrong blood leading to fatal hemolysis due to transfusion. ANH is a cost-effective transfusion option for suitable patients. Appropriate clinical decision making, use of strategies to decrease peri-operative blood losses and cost-effective country based planning could be more widely applied to improve clinical transfusion practice.

  15. Frequencies of red blood cell major blood group antigens and phenotypes in the Chinese Han population from Mainland China.

    Science.gov (United States)

    Yu, Y; Ma, C; Sun, X; Guan, X; Zhang, X; Saldanha, J; Chen, L; Wang, D

    2016-08-01

    Alloantibodies directed to red blood cell (RBC) antigens play an important role in alloimmune-mediated haemolytic transfusion reactions and haemolytic disease of the foetus and newborn. The frequencies and phenotypes of RBC antigens are different in populations from different geographic areas and races. However, the data on major blood group antigens in the Chinese Han population from Mainland China are still very limited; thus, we aimed to investigate them in this study. A total of 1412 unrelated voluntary Chinese Han blood donors were randomly recruited. All donors were typed for blood group antigens: D, C, c, E, e, C(w) , Jk(a) , Jk(b) ,M, N, S, s, Le(a) , Le(b) , K, k. Kp(a) , Kp(b) , Fy(a) , Fy(b) , Lu(a) , Lu(b) , P1 and Di(a) using serological technology. Calculations of antigen and phenotype frequencies were expressed as percentages and for allele frequencies under the standard assumption of Hardy-Weinberg equilibrium. Amongst the Rh antigens, D was the most common (98.94%) followed by e (92.28%), C (88.81%), c (58.43%), E (50.78%) and C(w) (0.07%) with DCe/DCe (R1 R1 , 40.72%) being the most common phenotype. In the Kell blood group system, k was present in 100% of the donors and a rare phenotype, Kp (a+b+), was found in 0.28% of the donors. For the Kidd and Duffy blood group systems, Jk (a+b+) and Fy (a+b-) were the most common phenotypes (44.05% and 84.35%, respectively). In the MNS blood group system, M+N+S-s+ (45.54%) was the most common, whereas M+N-S-s- and M-N+S-s- were not found. The rare Lu (a-b-) and Lu (a+b+) phenotypes were identified in 0.43% and 1.13% of the donors, respectively. Le(a) and Le(b) were seen in 17.92% and 63.03% of donors, respectively. The frequency of Di(a) was 4.75%, which was higher than in the Chinese population in Taiwan region or the Caucasian and Black populations (P < 0.0001). This study systematically describes the frequencies of 24 blood group antigens in the Chinese Han population from Mainland China. The data can

  16. The importance of blood lipids in the association between BMI and blood pressure among Chinese overweight and obese children.

    Science.gov (United States)

    Zou, Zhi-Yong; Yang, Yi-de; Wang, Shuo; Dong, Bin; Li, Xiao-Hui; Ma, Jun

    2016-07-01

    We aimed to examine the contribution of blood lipids to the association between BMI and blood pressure (BP) in children with overweight and obesity. Data were collected in elementary and high schools of Chaoyang District, Beijing, China in 2012. Participants' weight, height, BP and fasting plasma lipid profile were measured by standard protocols. Mediation analysis was used to examine the mediation role of blood lipids on the relation between BMI and BP, with age included as a covariate. We found that in boys 8·29 % (mediation effect=0·106, P=0·012) of the association between BMI and systolic BP was mediated through TAG. TAG mediated 12·53 % (mediation effect=0·093, P=0·018) and LDL-cholesterol mediated 7·75 % (mediation effect=0·57, P=0·046) of the association between BMI and diastolic BP was mediated by TAG and LDL-cholesterol, respectively. However, blood lipids did not show the mediation effect in girls. Our findings suggested that there was a sex difference in the contribution of blood lipids to the association between BMI and BP. Controlling TAG or LDL-cholesterol may be beneficial for reducing the risk of the BMI-related high BP in overweight boys; however, this outcome is not the case when controlling TAG or LDL-cholesterol in girls. This study may provide clues to explore the underlying mechanism of the association between obesity and hypertension. PMID:27169905

  17. Blood lipid levels associate with childhood asthma, airway obstruction, bronchial hyperresponsiveness, and aeroallergen sensitization

    DEFF Research Database (Denmark)

    Vinding, Rebecca K; Stokholm, Jakob; Chawes, Bo L K;

    2016-01-01

    BACKGROUND: Studies of children's blood lipid profiles in relation to asthma are few, and the results are ambiguous. OBJECTIVE: We sought to examine whether the lipid profile is associated with concurrent asthma, altered lung function, and allergic sensitization in children. METHODS: High...... systemic disorders with commonalities with other chronic inflammatory disorders....

  18. Blood pressure, heart rate and lipids in professional handball and water polo players

    OpenAIRE

    Jovanović Jovica; Jovanović Milan

    2005-01-01

    Introduction: Blood pressure, heart rate and lipoprotein lipids are affected by family history, obesity, diet, smoking and physical activity habits. The aim of this paper was to estimate the values of blood pressure and heart rate in professional handball and water polo players before and after training and submaximal exercise test and to analyze the lipid state in these professional athletes in comparison with people who have never been in sports. Material and methods The investigation inclu...

  19. Metabolic phenotyping by 1H-NMR spectroscopy detects lung cancer via a simple blood sample

    OpenAIRE

    Louis,Evelyne; MESOTTEN, Liesbet; Thomeer, Michiel; Vandeurzen, Kurt; Darquennes, Karen; Vanhove, Karolien; Reekmans, Gunter; Adriaensens, Peter

    2013-01-01

    Introduction: Lung cancer is the leading cause of cancer death worldwide. There is an urgent need of effective methods to detect lung cancer. Accumulating evidence shows that the metabolism of cancer cells differs from that of normal cells. Disturbances in biochemical pathways which occur during the development of cancer provoke changes in the metabolic phenotype. Objective: To determine the metabolic phenotype of lung cancer by 1H-NMR spectroscopy. Methods: Fasting venous blood samples of 78...

  20. Multiple loci are associated with white blood cell phenotypes.

    Directory of Open Access Journals (Sweden)

    Michael A Nalls

    2011-06-01

    Full Text Available White blood cell (WBC count is a common clinical measure from complete blood count assays, and it varies widely among healthy individuals. Total WBC count and its constituent subtypes have been shown to be moderately heritable, with the heritability estimates varying across cell types. We studied 19,509 subjects from seven cohorts in a discovery analysis, and 11,823 subjects from ten cohorts for replication analyses, to determine genetic factors influencing variability within the normal hematological range for total WBC count and five WBC subtype measures. Cohort specific data was supplied by the CHARGE, HeamGen, and INGI consortia, as well as independent collaborative studies. We identified and replicated ten associations with total WBC count and five WBC subtypes at seven different genomic loci (total WBC count-6p21 in the HLA region, 17q21 near ORMDL3, and CSF3; neutrophil count-17q21; basophil count- 3p21 near RPN1 and C3orf27; lymphocyte count-6p21, 19p13 at EPS15L1; monocyte count-2q31 at ITGA4, 3q21, 8q24 an intergenic region, 9q31 near EDG2, including three previously reported associations and seven novel associations. To investigate functional relationships among variants contributing to variability in the six WBC traits, we utilized gene expression- and pathways-based analyses. We implemented gene-clustering algorithms to evaluate functional connectivity among implicated loci and showed functional relationships across cell types. Gene expression data from whole blood was utilized to show that significant biological consequences can be extracted from our genome-wide analyses, with effect estimates for significant loci from the meta-analyses being highly corellated with the proximal gene expression. In addition, collaborative efforts between the groups contributing to this study and related studies conducted by the COGENT and RIKEN groups allowed for the examination of effect homogeneity for genome-wide significant associations across

  1. Association of Polymorphisms of Genes Involved in Lipid Metabolism with Blood Pressure and Lipid Values in Mexican Hypertensive Individuals

    Directory of Open Access Journals (Sweden)

    Blanca Estela Ríos-González

    2014-01-01

    Full Text Available Hypertension and dyslipidemia exhibit an important clinical relationship because an increase in blood lipids yields an increase in blood pressure (BP. We analyzed the associations of seven polymorphisms of genes involved in lipid metabolism (APOA5 rs3135506, APOB rs1042031, FABP2 rs1799883, LDLR rs5925, LIPC rs1800588, LPL rs328, and MTTP rs1800591 with blood pressure and lipid values in Mexican hypertensive (HT patients. A total of 160 HT patients and 160 normotensive individuals were included. Genotyping was performed through PCR-RFLP, PCR-AIRS, and sequencing. The results showed significant associations in the HT group and HT subgroups classified as normolipemic and hyperlipemic. The alleles FABP2 p.55T, LIPC −514T, and MTTP −493T were associated with elevated systolic BP. Five alleles were associated with lipids. LPL p.474X and FABP2 p.55T were associated with decreased total cholesterol and LDL-C, respectively; APOA5 p.19W with increased HDL-C; APOA5 p.19W and FABP2 p.55T with increased triglycerides; and APOB p.4181K and LDLR c.1959T with decreased triglycerides. The APOB p.E4181K polymorphism increases the risk for HT (OR = 1.85, 95% CI: 1.17–2.93; P=0.001 under the dominant model. These findings indicate that polymorphisms of lipid metabolism genes modify systolic BP and lipid levels and may be important in the development of essential hypertension and dyslipidemia in Mexican HT patients.

  2. Abundant genetic overlap between blood lipids and immune-mediated diseases indicates shared molecular genetic mechanisms.

    Directory of Open Access Journals (Sweden)

    Ole A Andreassen

    Full Text Available Epidemiological studies suggest a relationship between blood lipids and immune-mediated diseases, but the nature of these associations is not well understood. We used genome-wide association studies (GWAS to investigate shared single nucleotide polymorphisms (SNPs between blood lipids and immune-mediated diseases. We analyzed data from GWAS (n~200,000 individuals, applying new False Discovery Rate (FDR methods, to investigate genetic overlap between blood lipid levels [triglycerides (TG, low density lipoproteins (LDL, high density lipoproteins (HDL] and a selection of archetypal immune-mediated diseases (Crohn's disease, ulcerative colitis, rheumatoid arthritis, type 1 diabetes, celiac disease, psoriasis and sarcoidosis. We found significant polygenic pleiotropy between the blood lipids and all the investigated immune-mediated diseases. We discovered several shared risk loci between the immune-mediated diseases and TG (n = 88, LDL (n = 87 and HDL (n = 52. Three-way analyses differentiated the pattern of pleiotropy among the immune-mediated diseases. The new pleiotropic loci increased the number of functional gene network nodes representing blood lipid loci by 40%. Pathway analyses implicated several novel shared mechanisms for immune pathogenesis and lipid biology, including glycosphingolipid synthesis (e.g. FUT2 and intestinal host-microbe interactions (e.g. ATG16L1. We demonstrate a shared genetic basis for blood lipids and immune-mediated diseases independent of environmental factors. Our findings provide novel mechanistic insights into dyslipidemia and immune-mediated diseases and may have implications for therapeutic trials involving lipid-lowering and anti-inflammatory agents.

  3. The associations of a marine diet with plasma lipids, blood glucose, blood pressure and obesity among the inuit in Greenland

    DEFF Research Database (Denmark)

    Bjerregaard, P; Pedersen, H S; Mulvad, G

    2000-01-01

    OBJECTIVE: To analyse the associations between the intake of fish and marine mammals and risk factors for cardiovascular disease, ie lipid profile, fasting blood glucose, blood pressure and obesity, in a population whose average consumption of n-3 fatty acids is high compared with Western countries...... statistically significant. The pattern was similar within groups with low, medium and high consumption of marine food. CONCLUSIONS: There are statistically significant associations between the consumption of marine food and certain lipid fractions in the blood also in this population with a very high average...... intake of marine food. The observation that blood glucose is positively associated with marine diet in a population survey is new and should be repeated. There was good agreement between the results for the reported consumption of seal and those for the biomarkers. SPONSORSHIP: The study was financially...

  4. Phenotypic, functional, and quantitative characterization of canine peripheral blood monocyte-derived macrophages

    Directory of Open Access Journals (Sweden)

    R Bueno

    2005-08-01

    Full Text Available The yield as well as phenotypic and functional parameters of canine peripheral blood monocyte-derived macrophages were analyzed. The cells that remained adherent to Teflon after 10 days of culture had high phagocytic activity when inoculated with Leishmania chagasi. Flow cytometric analysis demonstrated that more than 80% of cultured cells were positive for the monocyte/macrophage marker CD14.

  5. Effect of oils sources on blood lipid parameters of commercial laying hens

    Directory of Open Access Journals (Sweden)

    LS Murata

    2003-12-01

    Full Text Available The experiment was carried out to verify if total cholesterol, HDL-cholesterol and triacylglicerol plasma levels are affected when laying hens are fed rations containing different dietary oil sources. One hundred sixty 50 week-old hens, assigned to four treatments with five replicates using 8 hens per replicate were used. The experimental period was of 84 days divided in 3 cycles of 28 days each. In the last day of each cycle, blood samples of 2 hens per replicate were randomly choose and blood samples were collected. On the other hand, blood was also collected at 7 am, 11 am and 3 pm aiming to study the daily changes of these lipids. Blood lipid parameters were not affected by different dietary oil sources (p > 0.05; however, HDL-cholesterol did change during the day, giving evidence that this lipid is indeed involved in the egg yolk formation.

  6. Fish oil affects blood pressure and the plasma lipid profile in healthy Danish infants

    DEFF Research Database (Denmark)

    Damsgaard, C.T.; Schack-Nielsen, L.; Michaelsen, K.F.; Fruekilde, Maj-Britt; Hels, O.; Lauritzen, L.

    2006-01-01

    Animal and epidemiologic studies indicate that early nutrition has lasting effects on metabolism and cardiovascular disease risk. In adults, (n-3) long-chain PUFA (LCPUFA) from fish oils improve blood pressure, the lipid profile, and possibly cardiovascular disease mortality. This randomized trial...... is the first to investigate the effects of fish oil on blood pressure and the lipid profile in infancy. Healthy term 9-mo old infants In 83) were randomly assigned to 5 mL fish oil daily or no fish oil for 3 mo and to 2 different milk types. Before and after the intervention, blood pressure was...... measured with an oscillometric device, and blood was sampled for analysis of erythrocyte fatty acid composition and the plasma lipid profile. This paper examines the effects of the fish oil supplement, with adjustment for the effects of the milk intervention when relevant. The fish oil intervention...

  7. Effects of Different Exercise Intensities with Isoenergetic Expenditures on C-Reactive Protein and Blood Lipid Levels

    Science.gov (United States)

    Tsao, Te Hung; Yang, Chang Bin; Hsu, Chin Hsing

    2012-01-01

    We investigated the effects of different exercise intensities on C-reactive protein (CRP), and whether changes in CRP levels correlated with blood lipid levels. Ten men exercised at 25%, 65%, and 85% of their maximum oxygen consumption rates. Participants' blood was analyzed for CRP and blood lipid levels before and after the exercise sessions.…

  8. Relationship of endothelial nitric oxide synthase gene polymorphism with blood pressure,lipid profile and blood glucose level

    Institute of Scientific and Technical Information of China (English)

    2008-01-01

    To study the relationship of the polymorphism of endothelial nitric oxide synthase(eNOS)gene and blood pressure,lipid profiles and blood glucose level.By using PCR-RFLP,the eNOS Glu298Asp gene polymorphism was detected in 184 patients with essential hypertension and 196 matched healthy individuals with normal blood pressure.Taking into account eNOS Glu298Asp polymorphisms,the relationship of blood pressure with triglycerides(TG),total cholesterol(TC),high density lipoprotein(HDL),low density lipoprotein(LDL)and blood glucose level was analyzed.The distribution of eNOS Glu298Asp polymorphism had no significant difference between different blood pressure groups and gender groups,but there was a significant difference between different age groups,diastolic blood pressure groups or BMI groups(P<0.05).Asp/Asp genotype significantly increased the risk of hypertension in individuals with serum TC above 5.4 mmol/L(P=0.03,OR=2.65).eNOSGlu298Asp polymorphism and serum lipid could synergistically modulate the blood pressure,eNOS Asp/Asp genotype could significantly increase the risk of hypertension in individuals with serum TC over 5.4 mmol/L,eNOS Glu298Asp in combination with serum TC could be used to predict the risk of hypertension.

  9. Lipid composition of the human eye: are red blood cells a good mirror of retinal and optic nerve fatty acids?

    OpenAIRE

    Acar, Niyazi; Berdeaux, Olivier; Grégoire, Stéphane; Cabaret, Stéphanie; Martine, Lucy; Gain, Philippe; Thuret, Gilles; Creuzot Garcher, Catherine; Bron, Alain; Bretillon, Lionel

    2012-01-01

    BACKGROUND: The assessment of blood lipids is very frequent in clinical research as it is assumed to reflect the lipid composition of peripheral tissues. Even well accepted such relationships have never been clearly established. This is particularly true in ophthalmology where the use of blood lipids has become very common following recent data linking lipid intake to ocular health and disease. In the present study, we wanted to determine in humans whether a lipidomic approach based on red bl...

  10. Synergistic effects of elevated homocysteine level and abnormal blood lipids on the onset of stroke

    Institute of Scientific and Technical Information of China (English)

    Lu Hao; Zhenzhen Cheng; Qiang Wu; Shuzhang Li; Liming Chen; Xiaoyong Sai; Zhefeng Liu; Guang Yang; Rongzeng Yan; Lili Wang; Caiyun Fu; Xuan Xu

    2013-01-01

    Hyperhomocysteinemia and abnormal blood lipids are independent risk factors for stroke. However, whether both factors exert a synergistic effect in the onset of stroke remains unclear. The present study is a retrospective analysis of 2 089 cases of stroke and 2 089 control cases of simple inter-vertebral disk protrusion using a paired multivariate logistic regression method. Adjusting for known confounding variables including the patients’ age, gender, smoking status, alcohol consumption status, patient and family medical history, and clinical biochemical indices, elevated homocysteine level was related to the onset of stroke. Patients with elevated homocysteine levels and abnormal blood lipids showed a 40.9%increase in the risk for stroke compared to patients with normal ho-mocysteine levels and blood lipids (odds ratio 1.409;95%confidence interval 1.127-1.761). These results indicate that elevated homocysteine and abnormal blood lipids exert synergistic effects in the onset of stroke. Patients with elevated homocysteine levels and abnormal blood lipids are predis-posed to stroke.

  11. The BUME method: a novel automated chloroform-free 96-well total lipid extraction method for blood plasma[S

    OpenAIRE

    Löfgren, Lars; Ståhlman, Marcus; Forsberg, Gun-Britt; Saarinen, Sinikka; Nilsson, Ralf; Göran I Hansson

    2012-01-01

    Lipid extraction from biological samples is a critical and often tedious preanalytical step in lipid research. Primarily on the basis of automation criteria, we have developed the BUME method, a novel chloroform-free total lipid extraction method for blood plasma compatible with standard 96-well robots. In only 60 min, 96 samples can be automatically extracted with lipid profiles of commonly analyzed lipid classes almost identically and with absolute recoveries similar or better to what is ob...

  12. Non-ABO blood group systems phenotyping in non-human primates for blood banking laboratory and xenotransplantation.

    Science.gov (United States)

    Ramis, G; Martínez-Alarcon, L; Quereda, J J; Mrowiec, A; Funes, C; Ríos, A; Ramírez, P; Muñoz, A; Majado, M J

    2013-04-01

    Some biomedical research procedures, such as organ xenotransplantation, usually require intensive hemotherapy. Knowledge of the whole phenotype of blood donor and graft could be useful in the field of xenotransplantation. Human and simian-type categories of blood groups have been established and they can be tested by standard methods used for human blood grouping. The aim of this work was to study the incidence of non-ABO blood group systems in different species of non-human primates, which are employed in biomedical research. The phenotype of Rh, Lewis, Kidd, Kell, MNSs, Lutheran, P and Duffy antigens was investigated in olive baboon (n = 48), chacma baboon (n = 9), Guinea baboon (n = 14), Rhesus macaque (n = 38) and squirrel monkey (n = 30) by using commercial microtyping cards. Kell, Lutheran, Kidd and Duffy antigens have been detected in all species, Rh in squirrel monkey, MNSs in rhesus macaque and squirrel monkey, and Lewis in baboon and rhesus macaque. There were differences in frequency and haemagglutination scores between species regardless of their gender and age. The main differences were found in squirrel monkey when compared with baboons and macaques. This typing system provides a tool to assess the presence of antigens in animals used for experimental procedures, such as xenotransplantation and xenotransfusion. PMID:23563364

  13. Aluminum induces lipid peroxidation and aggregation of human blood platelets

    OpenAIRE

    T.J.C. Neiva; D.M. Fries; Monteiro, H. P.; E.A. D'Amico; D.A.F. Chamone

    1997-01-01

    Aluminum (Al3+) intoxication is thought to play a major role in the development of Alzheimer's disease and in certain pathologic manifestations arising from long-term hemodialysis. Although the metal does not present redox capacity, it can stimulate tissue lipid peroxidation in animal models. Furthermore, in vitro studies have revealed that the fluoroaluminate complex induces diacylglycerol formation, 43-kDa protein phosphorylation and aggregation. Based on these observations, we postulated t...

  14. The circulating PBEF/NAMPT/visfatin level is associated with a beneficial blood lipid profile

    OpenAIRE

    Wang, P.; Greevenbroek, van, M.M.J.; Bouwman, F.G. (F.G.); Brouwers, M.C.G.J.; Kallen, van der, B.F.; Smit, E.; Keijer, J.; Mariman, E C M

    2007-01-01

    Visfatin with the official gene name pre-B cell colony-enhancing factor 1 (PBEF) and the protein name nicotinamide phosphoribosyltransferase (NAMPT) is a recently discovered adipocyte-secreted protein that was shown by some to be associated with visceral fat and insulin resistance. To explore the link between PBEF/NAMPT/visfatin and lipid metabolism, we analyzed the relation of its plasma level with several parameters of adiposity, insulin resistance and the circulating blood lipid profile in...

  15. FADS1 FADS2 gene cluster, PUFA intake and blood lipids in children

    OpenAIRE

    Standl, Marie; Lattka, Eva; Stach, Barbara; Koletzko, Sibylle; Bauer, Carl-Peter; Berg, Andrea von; Berdel, Dietrich; Krämer, Ursula; Schaaf, Beate; Röder, Stefan; Herbarth, Olf; Buyken, Anette; Drogies, Tim; Thiery, Joachim; Koletzko, Berthold

    2012-01-01

    Elevated cholesterol levels in children can be a risk factor for cardiovascular diseases in later life. In adults, it has been shown that blood lipid levels are strongly influenced by polymorphisms in the fatty acid desaturase (FADS) gene cluster in addition to nutritional and other exogenous and endogenous determinants. Our aim was to investigate whether lipid levels are determined by the FADS genotype already in children and whether this association interacts with dietary intake of n-3 fatt...

  16. Oxidation of Lipids and Proteins in Lens and Blood Plasma of Rats in Ageing

    Directory of Open Access Journals (Sweden)

    Ivanova I.P.

    2011-09-01

    Full Text Available The aim of the study is to assess the intensity of oxidation of lipids and proteins in lens and blood plasma of Wistar rats in ageing. Materials and Methods. The experiments were carried out on 25 Wistar male rats of four age groups: 5, 12, 24 and 36 months. Materials for study were lens and blood plasma. Lipids were extracted using Folch partition. The content of diene and triene conjugates was assessed by means of spectrophotometry. The level of Schiff’s bases was studied according to fluorescence intensity, malon dialdehyde concentration — according to the intensity of interaction with thiobarbituric acid. Potentiality of substrate oxidation in specimen was assessed using the method of induced chemoluminescence, and the degree of protein oxidative modification was assessed according to the level of carbonyl derivatives with 2.4-dinitrophenylhydrasine. The investigation of the content of total lipids and total proteins were carried out using “Bio-Test Total Lipids” and “Total Protein-Vital”. Results. The processes of lipid peroxidation of lens membranes are increasing in animals aged 5—12 months and decreasing in the period of 12—24 months. The level of lipid peroxidation in blood plasma has an expressed tendency for increasing in ageing. Over the years, there is the level decrease of carbonyl derivatives of aminoacids of lens proteins and the tendency for the increase of oxidative modification of proteins in blood plasma.

  17. Blood Lipid Profile and Prevalence of Dyslipidemia in Chinese Adults

    Institute of Scientific and Technical Information of China (English)

    2007-01-01

    Objective To investigate the plasma lipid levels in a national representative sample of subjects and to determine the prevalence of dyslipidemia in the Chinese population. Methods Plasma lipid profile was analyzed using the data obtained during the Chinese national nutrition and health survey (CNHS) in 2002 which involved 14 252 participants at the age of 18 years or older. Results The mean levels of total cholesterol (TC), triglyceride (TG) and high density lipoprotein cholesterol (HDL-C) in the participants were 3.81 mmol/L, 1.10 mmol/L, and 1.30 mmol/L, respectively. In the groups of participants at the age of 18-44 years, 45-59 years, and over 60 years the mean TC level was 3.70 mmol/L, 4.09 mmol/L and 4.21 mmol/L,respectively, and the mean TG level was 07 mmol/L, 1.21 mmol/L, 1.20 mmol/L, 1.29 mmol/L, 1.33 mmol/L, and 1.33 mmol/L,respectively. The prevalence of dyslipidemia in Chinese adults was 18.6% and 22.2% in males and 15.9% in females.Dyslipidemia prevalence was higher in urban districts than in rural areas (21.0% vs. 17.7%). The prevalence of hypercholesterolemia, hypertriglyceridemia, and low HDL cholesterol was 2.9%, 11.9%, and 7.4% respectively among the participants. Conclusion Dyslipidemia has become one of the important health risk factors in the Chinese population. There is no significantly difference in the prevalence of dyslipidemia between the groups of participants at the age of 45-59 years and over 60 years. This study provides important lipid profile data for policy making and guideline development for the prevention of dyslipidemia in the Chinese population.

  18. Removal of. cap alpha. -tocopherol from blood and its comparison with other lipids

    Energy Technology Data Exchange (ETDEWEB)

    Gardner, H.K.; Vang, M.J.; Mavis, R.D.

    1986-01-01

    The blood decay curve of ..cap alpha..-tocopherol in rats was compared with those of the two major blood lipids by labeling rat serum in vitro with /sup 3/H-..cap alpha..-tocopherol (AT), /sup 3/H-cholesterol (CHO) or /sup 3/H-trioleoylglycerol (TO) and injecting it into the bloodstream. For the three lipids, loss from blood was biphasic. The half time of the faster decay was 2-4 minutes. The slower curve decayed with half times of 42, 289 and 990 minutes for TO, AT and CHO, respectively. This intermediate rate of AT removal is consistent with its accompanying both of the major blood lipids as they are removed by their respective mechanisms or with a process specific for AT. To investigate the role of liver in the faster curve, animals were hepatectomized. TO and CHO loss remained biphasic after liver removal. However, AT loss became monophasic, with a loss rate intermediate between the non-hepatectomized fast and slow decays. This demonstrates a central role for liver in the metabolism of blood-borne AT and a mode of removal distinct from the other two lipids.

  19. Immuno phenotype of blood lymphocytes in radiation-associated Hodgkin's disease

    International Nuclear Information System (INIS)

    Immuno phenotype of peripheral blood lymphocytes has been studied in Hodgkin's disease including patients exposed to radionuclides of the characteristic Chernobyl pattern. The group of patients under study has been characterized by decreasing T- and NK-cell immunity, such a decrease being more pronounced in radiation-associated Hodgkin's lymphoma. The data obtained as well as the evidence of Epstein-Barr virus activation could explain the aggressiveness of the disease in such patients and the difficulties in their treatment

  20. 24-hour central blood pressure and intermediate cardiovascular phenotypes in untreated subjects

    OpenAIRE

    Bednarek, Agnieszka; Jankowski, Piotr; Olszanecka, Agnieszka; Windak, Adam; Kawecka-Jaszcz, Kalina; Czarnecka, Danuta

    2014-01-01

    Background: Recently, 24-hour monitoring of central systolic blood pressure (SBP) has become available. However, the relation between end-organ damage and the 24-hour central SBP profile and variability has not so far been analyzed. Therefore, the aim of this cross-sectional study was to evaluate the relation between 24-hour central SBP, 24-hour central SBP profile as well as central SBP short-term variability and parameters of cardiac and vascular intermediate phenotypes. Methods: The study ...

  1. The effects of coffee on serum lipids and blood pressure in a UK population.

    OpenAIRE

    Lancaster, T; Muir, J; Silagy, C

    1994-01-01

    Data on coffee consumption were collected for 1074 adults attending for health checks in the OXCHECK study, to assess associations with serum lipids and blood pressure. Of the sample, 70.5% drank coffee, largely instant. Coffee had no significant effects on total or high-density lipoprotein (HDL) cholesterol or blood pressure, and was negatively correlated with serum triglycerides. The type of coffee drunk in the UK does not adversely effect these cardiovascular risk factors.

  2. Top-down lipidomics reveals ether lipid deficiency in blood plasma of hypertensive patients.

    Directory of Open Access Journals (Sweden)

    Juergen Graessler

    Full Text Available BACKGROUND: Dyslipoproteinemia, obesity and insulin resistance are integrative constituents of the metabolic syndrome and are major risk factors for hypertension. The objective of this study was to determine whether hypertension specifically affects the plasma lipidome independently and differently from the effects induced by obesity and insulin resistance. METHODOLOGY/PRINCIPAL FINDINGS: We screened the plasma lipidome of 19 men with hypertension and 51 normotensive male controls by top-down shotgun profiling on a LTQ Orbitrap hybrid mass spectrometer. The analysis encompassed 95 lipid species of 10 major lipid classes. Obesity resulted in generally higher lipid load in blood plasma, while the content of tri- and diacylglycerols increased dramatically. Insulin resistance, defined by HOMA-IR >3.5 and controlled for BMI, had little effect on the plasma lipidome. Importantly, we observed that in blood plasma of hypertensive individuals the overall content of ether lipids decreased. Ether phosphatidylcholines and ether phosphatidylethanolamines, that comprise arachidonic (20:4 and docosapentaenoic (22:5 fatty acid moieties, were specifically diminished. The content of free cholesterol also decreased, although conventional clinical lipid homeostasis indices remained unaffected. CONCLUSIONS/SIGNIFICANCE: Top-down shotgun lipidomics demonstrated that hypertension is accompanied by specific reduction of the content of ether lipids and free cholesterol that occurred independently of lipidomic alterations induced by obesity and insulin resistance. These results may form the basis for novel preventive and dietary strategies alleviating the severity of hypertension.

  3. THE IMPACT OF FAST FOOD CONSUMPTION ON THE LIPID PROFILE, BMI AND BLOOD SUGAR LEVELS

    Directory of Open Access Journals (Sweden)

    Afroz Afshan*, Veeresh B Salgar, Manjushree Sugoor and Anupriya Deshpande

    2014-07-01

    Full Text Available In India smoking, alcohol consumption, sedentary life style and junk foods are risk factors that has increased the burden of cardiovascular disease. To evaluate and compare the lipid profile, BMI and blood sugar levels in vegetarian home food eaters and fast food eaters. Apparently healthy 300 males aged between 15-35years were participants of our study. Out of them 150 vegetarian home food eaters formed control group, 150 fast food eaters formed study group. We observed over all highly significant effect of fast food in study group compared to control group. In our study we found that BMI, RBS and lipid profile was significantly increased with p value <0.001 in the study group. With regular consumption of fast food there is a derangement of lipid function and increased blood sugar leading to early obesity and ill health. Hence the habit of fast food consumption should be curbed at the earliest.

  4. Effect of cinnamon extract on blood glucose level and lipid profile in alloxan induced diabetic rats

    International Nuclear Information System (INIS)

    Background: Cinnamon has been shown to potentiate the hypoglycaemic effect of insulin through up regulation of the glucose uptake in cultured adipocytes of rats. This study tried to find out the effect of Cinnamon alone or in combination with Insulin in diabetic albino rats. Methods: Thirty rats were divided into three groups, A and B. Group A were given cinnamon extract 200 mg/Kg body weight daily orally and group B rats were given cinnamon extract 400 mg/Kg body weight daily. After six weeks blood glucose and lipid profile levels were evaluated in all the groups. Results: Group of rats given 200 mg cinnamon extract showed significant decrease of blood glucose concentration but there was slight or no change in the level of lipid parameters including serum cholesterol, triglyceride and lipoproteins (HDL, LDL-chol). On the other hand group of rats given 400 mg extract of cinnamon showed a better but non significant change in level of lipid related parameter while blood glucose level was significantly decreased. Conclusion: The cinnamon at a dose of 400 mg showed same effects on blood glucose level but better effects on lipid profiles especially of serum cholesterol level of group of rats compared to 200 mg of cinnamon extract. Cinnamon may be recommended as hypoglycaemic herb but not as hypolipidaemic herb. (author)

  5. Calibration of a prototype NIRS oximeter against two commercial devices on a blood-lipid phantom

    DEFF Research Database (Denmark)

    Hyttel-Sorensen, Simon; Kleiser, Stefan; Wolf, Martin; Greisen, Gorm

    2013-01-01

    In a blood-lipid liquid phantom the prototype near-infrared spectroscopy oximeter OxyPrem was calibrated against the INVOS® 5100c adult sensor in respect to values of regional tissue oxygen haemoglobin saturation (rStO2) for possible inclusion in the randomised clinical trial - SafeBoosC. In...

  6. Mendelian randomization of blood lipids for coronary heart disease

    Science.gov (United States)

    Holmes, Michael V.; Asselbergs, Folkert W.; Palmer, Tom M.; Drenos, Fotios; Lanktree, Matthew B.; Nelson, Christopher P.; Dale, Caroline E.; Padmanabhan, Sandosh; Finan, Chris; Swerdlow, Daniel I.; Tragante, Vinicius; van Iperen, Erik P.A.; Sivapalaratnam, Suthesh; Shah, Sonia; Elbers, Clara C.; Shah, Tina; Engmann, Jorgen; Giambartolomei, Claudia; White, Jon; Zabaneh, Delilah; Sofat, Reecha; McLachlan, Stela; Doevendans, Pieter A.; Balmforth, Anthony J.; Hall, Alistair S.; North, Kari E.; Almoguera, Berta; Hoogeveen, Ron C.; Cushman, Mary; Fornage, Myriam; Patel, Sanjay R.; Redline, Susan; Siscovick, David S.; Tsai, Michael Y.; Karczewski, Konrad J.; Hofker, Marten H.; Verschuren, W. Monique; Bots, Michiel L.; van der Schouw, Yvonne T.; Melander, Olle; Dominiczak, Anna F.; Morris, Richard; Ben-Shlomo, Yoav; Price, Jackie; Kumari, Meena; Baumert, Jens; Peters, Annette; Thorand, Barbara; Koenig, Wolfgang; Gaunt, Tom R.; Humphries, Steve E.; Clarke, Robert; Watkins, Hugh; Farrall, Martin; Wilson, James G.; Rich, Stephen S.; de Bakker, Paul I.W.; Lange, Leslie A.; Davey Smith, George; Reiner, Alex P.; Talmud, Philippa J.; Kivimäki, Mika; Lawlor, Debbie A.; Dudbridge, Frank; Samani, Nilesh J.; Keating, Brendan J.; Hingorani, Aroon D.; Casas, Juan P.

    2015-01-01

    Aims To investigate the causal role of high-density lipoprotein cholesterol (HDL-C) and triglycerides in coronary heart disease (CHD) using multiple instrumental variables for Mendelian randomization. Methods and results We developed weighted allele scores based on single nucleotide polymorphisms (SNPs) with established associations with HDL-C, triglycerides, and low-density lipoprotein cholesterol (LDL-C). For each trait, we constructed two scores. The first was unrestricted, including all independent SNPs associated with the lipid trait identified from a prior meta-analysis (threshold P < 2 × 10−6); and the second a restricted score, filtered to remove any SNPs also associated with either of the other two lipid traits at P ≤ 0.01. Mendelian randomization meta-analyses were conducted in 17 studies including 62,199 participants and 12,099 CHD events. Both the unrestricted and restricted allele scores for LDL-C (42 and 19 SNPs, respectively) associated with CHD. For HDL-C, the unrestricted allele score (48 SNPs) was associated with CHD (OR: 0.53; 95% CI: 0.40, 0.70), per 1 mmol/L higher HDL-C, but neither the restricted allele score (19 SNPs; OR: 0.91; 95% CI: 0.42, 1.98) nor the unrestricted HDL-C allele score adjusted for triglycerides, LDL-C, or statin use (OR: 0.81; 95% CI: 0.44, 1.46) showed a robust association. For triglycerides, the unrestricted allele score (67 SNPs) and the restricted allele score (27 SNPs) were both associated with CHD (OR: 1.62; 95% CI: 1.24, 2.11 and 1.61; 95% CI: 1.00, 2.59, respectively) per 1-log unit increment. However, the unrestricted triglyceride score adjusted for HDL-C, LDL-C, and statin use gave an OR for CHD of 1.01 (95% CI: 0.59, 1.75). Conclusion The genetic findings support a causal effect of triglycerides on CHD risk, but a causal role for HDL-C, though possible, remains less certain. PMID:24474739

  7. Genetic and phenotypic relationships between blood gas parameters and ascites-related traits in broilers.

    Science.gov (United States)

    Closter, A M; van As, P; Groenen, M A M; Vereijken, A L J; van Arendonk, J A M; Bovenhuis, H

    2009-03-01

    Ascites, also called pulmonary hypertension syndrome, is a metabolic disorder in chickens that have an insufficient pulmonary vascular capacity. The tendency of broilers to develop ascites is heritable, and successful selection against this susceptibility would benefit from good and easy-to-measure indicator traits. Blood gas parameters have been suggested as indicator traits for ascites susceptibility. Therefore, the aim of the present study was to estimate the heritability of blood gas parameters and the genetic and phenotypic correlations between blood gas parameters, heart ratio (postmortem indicator for ascites), and BW at 2 different ages. For this purpose, blood gas parameters, including the partial pressure of carbon dioxide in venous blood (pvCO(2)), the partial pressure of oxygen in venous blood (pvO(2)), and blood oxygen saturation, were measured at an average age of 22 d in nearly 3,000 broilers. To challenge the resistance of the birds to ascites, they were kept under cold conditions. Heritability for heart ratio was 0.43, and the heritability estimates were low: 0.02 for pvCO(2), 0.03 for pvO(2), and 0.07 for blood oxygen saturation. The estimated heritability for pH was 0.15, for bicarbonate was 0.19, and for total carbon dioxide content was 0.19. The genetic correlations between heart ratio and total carbon dioxide content (0.31 +/- 0.15) and between heart ratio and bicarbonate (0.31 +/- 0.15) were moderate and positive. For pvO(2), the genetic correlation with heart ratio was stronger and negative (-0.62 +/- 0.21); however, this correlation could not be estimated accurately because of the low heritability of pvO(2). For pvCO(2), the genetic correlation with the heart ratio was close to zero (-0.04 +/- 0.45). Phenotypic correlations between traits were, in general, similar to the genetic correlations. Heritabilities for blood gas parameters and the genetic correlations between blood gas parameters and the heart ratio estimated in the present study

  8. Association of ABO Blood Group Phenotype and Allele Frequency with Chikungunya Fever

    Science.gov (United States)

    Rujirojindakul, Pairaya; Chongsuvivatwong, Virasakdi; Limprasert, Pornprot

    2015-01-01

    Background. The objective of this study was to investigate the association of the ABO blood group phenotype and allele frequency with CHIK fever. Methods. A rural community survey in Southern Thailand was conducted in August and September 2010. A total of 506 villagers were enrolled. Cases were defined as individuals having anti-CHIK IgG by hemagglutination ≥1 : 10. Results. There were 314 cases (62.1%) with CHIK seropositivity. Females were less likely to have positive anti-CHIK IgG with odds ratio (OR) (95% CI) of 0.63 (0.43, 0.93). All samples tested were Rh positive. Distribution of CHIK seropositivity versus seronegativity (P value) in A, B, AB, and O blood groups was 80 versus 46 (0.003), 80 versus 48 (0.005), 24 versus 20 (0.55), and 130 versus 78 (<0.001), respectively. However, chi-square test between ABO and CHIK infection showed no statistical significance (P = 0.76). Comparison of the ABO blood group allele frequency between CHIK seropositivity and seronegativity was not statistically significant. Conclusion. This finding demonstrated no association of the ABO blood group phenotypes and allele frequencies with CHIK infection. PMID:25977691

  9. Effect of antioxidant rich diets on lipid profile and blood pressure in cardiovascular patients

    International Nuclear Information System (INIS)

    A sample of 200 patients was randomly selected and interviewed. Various data related to their food consumption in previous weeks and other behavioral attitudes were recorded. Their blood pressure was measured and blood was analyzed for total cholesterol (TC), high density lipoprotein cholesterol (HDL-C), low density lipoprotein cholesterol (LDL-C) and triglycerides (TG). Spearman's correlation coefficient was worked out between blood pressure, serum lipid parameters and tea, vitamin C and fibre intake. Tea showed significant correlation with diastolic blood pressure (DBP) (-0.2373; P<0.02), systolic blood pressure (SBP) (-0.2299; P<0.02) and TC (-0.3454; P<0.01). Vitamin C showed a negatively significant correlation with TC (-0.4676; P<0.01), and LDL-C (-2661; P<0.01) and significant positive correlation with HDL-C (+0.2227; P < 0.05). The tea intake was found strongly correlated with blood pressure as compared to vitamin C, while vitamin C had stronger correlation with TC as compared to tea intake. Fibre was not found significantly correlated with any of the studied parameters. A 30-day control trial on 50 subjects revealed that antioxidant therapy during fat-restricted diet period significantly affected blood pressure and serum lipids. Comparative effect showed that lemon juice showed best results. Lemon juice decreased DBP, SBP, TC, TG and increased HDL-C, while tea added with lemon only significantly decreased DBP, SBP and TC. Salad, especially onion, only improved HDL-C and LDLC levels. Vitamin C supplement also significantly lowered DBP, SBP, TC, LDL-C and TG. Tea had negative correlation with blood pressure and TC, while vitamin C has showed relationship with TC, LDL-C and positive with HDL-C. It is conceivable, therefore, that dietary antioxidants cause a significant improvement in blood pressure and serum lipids than vitamin C supplement and simple fat-restricted diets. (author)

  10. Inhibition of endotoxin-induced interleukin-6 production by synthetic lipid A partial structures in human peripheral blood mononuclear cells.

    OpenAIRE

    Wang, M. H.; Flad, H D; Feist, W; Brade, H; Kusumoto, S; Rietschel, E T; Ulmer, A J

    1991-01-01

    The effect of two synthetic lipid A partial structures, compound 406 (or LA-14-PP, identical in structure to the lipid A precursor, known as Ia or IVa) and compound 401 (lipid X), on the in vitro modulation of endotoxin (lipopolysaccharide)-induced interleukin-6 production by human blood mononuclear cells was investigated. Lipopolysaccharide of Salmonella abortus equi and synthetic Escherichia coli-type lipid A (compound 506, or LA-15-PP) had potent interleukin-6-inducing capacities. The maxi...

  11. Impact of dietary oils and fats on lipid peroxidation in liver and blood of albino rats

    Institute of Scientific and Technical Information of China (English)

    Rafaat Mohamed Elsanhoty; Mohamed Fawzy Ramadan

    2014-01-01

    Objective:To investigate the effects of different dietary fat and oils (differing in their degree of saturation and unsaturation) on lipid peroxidation in liver and blood of rats. Methods:The study was conducted on 50 albino rats that were randomly divided into 5 groups of 10 animals. The groups were fed on dietary butter (Group I), margarine (Group II), olive oil (Group III), sunflower oil (Group IV) and corn oil (Group V) for 7 weeks. After 12 h of diet removal, livers were excised and blood was collected to measure malondialdehyde (MDA) levels in the supernatant of liver homogenate and in blood. Blood superoxide dismutase activity (SOD), glutathione peroxidase activity (GPx), serum vitamin E and total antioxidant capacity (TAC) levels were also measured to determine the effects of fats and oils on lipid peroxidation. Results: The results indicated that no significant differences were observed in SOD activity, vitamin E and TAC levels between the five groups. However, there was significant decrease of GPx activity in groups IV and V when compared with other groups. The results indicated that feeding corn oil caused significant increases in liver and blood MDA levels as compared with other oils and fats. There were positive correlations between SOD and GPx, vitamin E and TAC as well as between GPx and TAC (r:0.743;P Conclusions:The results demonstrated that feeding oils rich in polyunsaturated fatty acids (PUFA) increases lipid peroxidation significantly and may raise the susceptibility of tissues to free radical oxidative damage.

  12. [Study of molecular mechanism for a blood sample with A3 phenotype].

    Science.gov (United States)

    Liang, Wei; Yang, Liang; Mei, Chuanliang; Xu, Deyi; Deng, Gang; He, Yunlei; Liu, Yiyu; Zhang, Zhe

    2015-10-01

    OBJECTIVE To explore the molecular mechanism for a blood sample with mixed-field hemagglutination upon determination of ABO blood group. METHODS Serological techniques were employed to identify the erythrocyte phenotype. The A and B antigens were detected by flow cytometry. The preliminary genotype of ABO gene was assayed with sequence-specific primer-polymerase chain reaction (PCR-SSP). Exons 6 and 7 of the ABO gene were amplified with PCR and analyzed by direct sequencing. Haplotypes of the ABO gene were analyzed by cloning sequencing as well. RESULTS The serological reaction pattern has supported an O phenotype when all the tubes were centrifuged for the first time. However, a mixed-field hemagglutination of red blood cells (RBCs) with anti-A antibodies was present after the tube was centrifuged five times later. A antigens were detected on the surface of partial red blood cells of the sample by flow cytometry. PCR- SSP results have shown that the preliminary ABO genotype was A/O. Analysis of the fragments of exons 6 and 7 of the ABO gene has indicated that heterozygosis lied as follows: 261G/A, 425T/T, 467C/T, 646A/T, 681A/G, 745C/T, 771C/T, 829A/G, conjecturing the genotype to be A307/O02, which was confirmed by haplotype sequence analysis. Compared with A101 allele, A307 allele has two missense mutations, 467C> T and 745C> T, which have resulted in substitutions Pro156Leu and Arg249Trp in the A glycosyltransferase polypeptide chain. CONCLUSION A variant allele (A307) has been identified for the first time in mainland China, which is responsible for the formation of A3 phenotype. PMID:26418996

  13. Determination of cardiac output, tissue blood flow, volume and lipid content in Sprague-Dawley rats

    International Nuclear Information System (INIS)

    One critical aspect of physiologically-based pharmacokinetic (PBPK) model development is the choice of values for organ blood flows, cardiac output and tissue volumes for input into models. These values vary depending upon the strain, size, age, and sex of animal for which a PBPK model is being developed. Tissue blood flows, cardiac output, tissue volume, and lipid content were determined in male S-D rats, (350-375 g, N=8). A radiolabel microsphere method utilizing Scandium (46Sc), Tin (113Sn),and Gadolinium (153Gd) was used to determine blood flow. Each rat received 3 radiolabeled injections. After the third injection, animals were sacrificed, and radioactivity in each tissue was determined in a 3-channel gamma counter. Tissues sampled include brain, heart, kidneys, liver, lungs, spleen, pancreas, adrenals, stomach, intestines, colon, testis, bone and skeletal muscle. Cardiac output was 142 ml/min. Blood flow values for eliminating organs were 0.49 (liver), 16.52 (kidney), and 1.77 (lung) ml/min/g tissue. Tissues which had significantly increased blood flow during the dark cycle included femur, abdonimal fat, triceps brachii and abdominal muscles, stomach, spleen and lung. Dissectable fat, organ volume, and organ lipid content were determined in a separate group of rats (N= 8). Volume and lipid content were determined for the same tissues as blood flow. Body fat was 7.35% of bw and extractable lipid content of eliminating organs was 42.3 (liver), 43.4 (kidney), and 35.9 (lung) mg/g tissue. Precise measurements should improve the accuracy of PBPK model predictions, and therfore help in reducing uncertainites in risk assessment of volatile organics and other pollutnats

  14. Association between blood lipid levels and personality traits in young Korean women.

    Directory of Open Access Journals (Sweden)

    Seung-Ju Roh

    blood lipid levels and personality traits may have a beneficial effect for the managing of dyslipidemia.

  15. Effectiveness of Shelled Almonds in Decreasing Blood Lipid and Lipoprotein Levels of Hyperlipidemic Patients

    Directory of Open Access Journals (Sweden)

    H Mozaffari

    2008-10-01

    Full Text Available Introduction: Hyperlipidemia is a risk factor for atherosclerosis and cardiovascular diseases. Nuts such as almonds are high in unsaturated lipids and antioxidants. Some studies indicate that nuts have beneficial effects on cardiovascular system. Therefore, the aim of this study was evaluation of the effectiveness of shelled almonds on reduction of blood lipid and lipoprotein levels in hyperlipidemic patients. Methods: This study was a clinical trial (before and after and was done on 30 men volunteering for the study. They consumed 60 grams shelled almonds per day for four weeks. Their blood lipid, lipoprotein, apolipoprotein and lipoprotein (a levels were measured after and before almond consumption. Results: Shelled almond consumption caused significant decrease in serum cholesterol 36.1 mg/dl, triglyceride 45.94 mg/dl, LDL-cholesterol 28.68 mg/dl and increase in HDL-cholesterol 10.64 mg/dl(p<0.001. Shelled almond consumption decreased lipoprotein (a (2.11 mg/dl, apolipoprotein B100 (8.93 mg/dl and increased apolipoprotein A (1 1.74 mg/dl levels, but this effect was insignificant statistically. Conclusion: Continuous consumption of shelled almonds has beneficial effect on blood lipids and may play a preventive role in Atherosclerosis and coronary heart diseases. We therefore suggest that a daily intake of 60 grams of almonds can be used for treatment of hyprelipidemic patients.

  16. Effects of Vegetarian Diets on Blood Lipids: A Systematic Review and Meta‐Analysis of Randomized Controlled Trials

    OpenAIRE

    Wang, Fenglei; Zheng, Jusheng; Yang, Bo; Jiang, Jiajing; Fu, Yuanqing; Li, Duo

    2015-01-01

    Background Vegetarian diets exclude all animal flesh and are being widely adopted by an increasing number of people; however, effects on blood lipid concentrations remain unclear. This meta‐analysis aimed to quantitatively assess the overall effects of vegetarian diets on blood lipids. Methods and Results We searched PubMed, Scopus, Embase, ISI Web of Knowledge, and the Cochrane Library through March 2015. Studies were included if they described the effectiveness of vegetarian diets on blood ...

  17. Impact of parenteral lipid emulsions on metabolomic phenotype in preterm TPN-fed piglets

    Science.gov (United States)

    Lipids in parenteral nutrition provide essential fatty acids and are a major source of energy for hospitalized neonates. Intralipid (IL) is the only approved lipid emulsion in the US, but new generation emulsions include Omegaven (OV) and SMOFlipid (SL). There are no studies describing the metabolit...

  18. Impact of parenteral lipid emulsions on the metabolomic phenotype in preterm TPN-fed piglets

    Science.gov (United States)

    Lipids in parenteral nutrition provide essential fatty acids and are a major source of energy for hospitalized neonates. Intralipid (IL) is the only approved lipid emulsion in the U.S, but new generation emulsions include Omegaven (OV) and SMOFlipid (SL). There are no studies describing the metaboli...

  19. Impact of dietary oils and fats on lipid peroxidation in liver and blood of albino rats

    Science.gov (United States)

    Haggag, Mohammad El-Sayed Yassin El-Sayed; Elsanhoty, Rafaat Mohamed; Ramadan, Mohamed Fawzy

    2014-01-01

    Objective To investigate the effects of different dietary fat and oils (differing in their degree of saturation and unsaturation) on lipid peroxidation in liver and blood of rats. Methods The study was conducted on 50 albino rats that were randomly divided into 5 groups of 10 animals. The groups were fed on dietary butter (Group I), margarine (Group II), olive oil (Group III), sunflower oil (Group IV) and corn oil (Group V) for 7 weeks. After 12 h of diet removal, livers were excised and blood was collected to measure malondialdehyde (MDA) levels in the supernatant of liver homogenate and in blood. Blood superoxide dismutase activity (SOD), glutathione peroxidase activity (GPx), serum vitamin E and total antioxidant capacity (TAC) levels were also measured to determine the effects of fats and oils on lipid peroxidation. Results The results indicated that no significant differences were observed in SOD activity, vitamin E and TAC levels between the five groups. However, there was significant decrease of GPx activity in groups IV and V when compared with other groups. The results indicated that feeding corn oil caused significant increases in liver and blood MDA levels as compared with other oils and fats. There were positive correlations between SOD and GPx, vitamin E and TAC as well as between GPx and TAC (r: 0.743; P<0.001) and between blood MDA and liver MDA (r: 0.897; P<0.001). The results showed also negative correlations between blood MDA on one hand and SOD, GPx, vitamin E and TAC on the other hand. Conclusions The results demonstrated that feeding oils rich in polyunsaturated fatty acids (PUFA) increases lipid peroxidation significantly and may raise the susceptibility of tissues to free radical oxidative damage. PMID:24144131

  20. The Autistic Phenotype Exhibits a Remarkably Localized Modification of Brain Protein by Products of Free Radical-Induced Lipid Oxidation

    OpenAIRE

    Evans, Teresa A.; Siedlak, Sandra L.; Liang Lu; Xiaoming Fu; Zeneng Wang; Woody R. McGinnis; Evelyn Fakhoury; Castellani, Rudy J.; Hazen, Stanley L.; Walsh, William J.; Allen T. Lewis; Salomon, Robert G.; Smith, Mark A.; George Perry; Xiongwei Zhu

    2008-01-01

    Oxidative damage has been documented in the peripheral tissues of autism patients. In this study, we sought evidence of oxidative injury in autistic brain. Carboxyethyl pyrrole (CEP) and iso[4]levuglandin (iso[4]LG)E2-protein adducts, that are uniquely generated through peroxidation of docosahexaenoate and arachidonate-containing lipids respectively, and heme oxygenase-1 were detected immunocytochemically in cortical brain tissues and by ELISA in blood plasma. Significant immunoreactivity tow...

  1. Circulating Blood Monocyte Subclasses and Lipid-Laden Adipose Tissue Macrophages in Human Obesity.

    Directory of Open Access Journals (Sweden)

    Tal Pecht

    Full Text Available Visceral adipose tissue foam cells are increased in human obesity, and were implicated in adipose dysfunction and increased cardio-metabolic risk. In the circulation, non-classical monocytes (NCM are elevated in obesity and associate with atherosclerosis and type 2 diabetes. We hypothesized that circulating NCM correlate and/or are functionally linked to visceral adipose tissue foam cells in obesity, potentially providing an approach to estimate visceral adipose tissue status in the non-surgical obese patient.We preformed ex-vivo functional studies utilizing sorted monocyte subclasses from healthy donors. Moreover, we assessed circulating blood monocyte subclasses and visceral fat adipose tissue macrophage (ATM lipid content by flow-cytometry in paired blood and omental-fat samples collected from patients (n = 65 undergoing elective abdominal surgery.Ex-vivo, NCM and NCM-derived macrophages exhibited lower lipid accumulation capacity compared to classical or intermediate monocytes/-derived macrophages. Moreover, of the three subclasses, NCM exhibited the lowest migration towards adipose tissue conditioned-media. In a cohort of n = 65, increased %NCM associated with higher BMI (r = 0.250,p<0.05 and ATM lipid content (r = 0.303,p<0.05. Among patients with BMI≥25Kg/m2, linear regression models adjusted for age, sex or BMI revealed that NCM independently associate with ATM lipid content, particularly in men.Collectively, although circulating blood NCM are unlikely direct functional precursor cells for adipose tissue foam cells, their increased percentage in the circulation may clinically reflect higher lipid content in visceral ATMs.

  2. Estimation of indices of lipid free-radical oxidation in blood plasma of irradiated sheep

    International Nuclear Information System (INIS)

    External α-irradiation of sheep with doses of 51.6 and 154.8 mC/kg initiated free-radical oxidation of lipids in blood plasma, which was characterized by increase of spontaneous chemiluminescence intensity, malonaldehyde, conjugated diene and triene as well as of α-tocopherol concentration, while retinol level simultaneously decreased. On the contrary, chronic irradiation of sheep with doses of 2.58 and 12.9 mC/kg resulted in decrease of malonaldehyde content, conjugated diene and triene level as well as natural antioxidant α-tocopherol level in blood plasma. 18 refs.; 2 figs.; 2 tabs

  3. Video Game Playing Is Independently Associated with Blood Pressure and Lipids in Overweight and Obese Adolescents

    OpenAIRE

    Goldfield, Gary S.; Kenny, Glen P.; Stasia Hadjiyannakis; Penny Phillips; Alberga, Angela S.; Saunders, Travis J.; Tremblay, Mark S.; Janine Malcolm; Denis Prud'homme; Rejeanne Gougeon; Sigal, Ronald J.

    2011-01-01

    OBJECTIVE: To examine the association between duration and type of screen time (TV, video games, computer time) and blood pressure (BP) and lipids in overweight and obese adolescents. DESIGN: This is a cross-sectional study of 282 overweight or obese adolescents aged 14-18 years (86 males, 196 females) assessed at baseline prior to beginning a lifestyle intervention study for weight control. Sedentary behaviours, defined as hours per day spent watching TV, playing video games, recreational co...

  4. Blood antioxidant profile and lipid peroxides in dairy cows with clinical mastitis

    OpenAIRE

    Rajesh Rathore; Vinod Kumar Gupta; Ricky Jhambh; Umesh Dimri

    2013-01-01

    Aim: To evaluate blood antioxidant profile and lipid peroxides in dairy cows with clinical mastitis. Materials and Methods: Twelve cases of clinical mastitis in cross-bred cows were selected based on physical examination of udder and milk, California Mastitis Test (CMT), Somatic Cell Count (SCC) and confirmation by bacteriological examination of milk and requisite biochemical tests. Twelve lactating cows showing negative CMT reaction and SCC <2x105 cells/ml were considered as healthy contr...

  5. THE IMPACT OF FAST FOOD CONSUMPTION ON THE LIPID PROFILE, BMI AND BLOOD SUGAR LEVELS

    OpenAIRE

    Afroz Afshan*, Veeresh B Salgar, Manjushree Sugoor and Anupriya Deshpande

    2014-01-01

    In India smoking, alcohol consumption, sedentary life style and junk foods are risk factors that has increased the burden of cardiovascular disease. To evaluate and compare the lipid profile, BMI and blood sugar levels in vegetarian home food eaters and fast food eaters. Apparently healthy 300 males aged between 15-35years were participants of our study. Out of them 150 vegetarian home food eaters formed control group, 150 fast food eaters formed study group. We observed over all highly signi...

  6. Relationships between membrane lipids and ion transport in red blood cells of Dahl rats

    Czech Academy of Sciences Publication Activity Database

    Vokurková, Martina; Nováková, O.; Dobešová, Zdenka; Kuneš, Jaroslav; Zicha, Josef

    2005-01-01

    Roč. 77, č. 13 (2005), s. 1452-1464. ISSN 0024-3205 R&D Projects: GA ČR(CZ) GA305/03/0769; GA MZd(CZ) NR7786; GA MŠk(CZ) 1M0510 Institutional research plan: CEZ:AV0Z50110509 Keywords : ion transport * membrane lipids * red blood cells Subject RIV: ED - Physiology Impact factor: 2.512, year: 2005

  7. Video Game Playing Is Independently Associated with Blood Pressure and Lipids in Overweight and Obese Adolescents

    OpenAIRE

    Gary S. Goldfield; Kenny, Glen P.; Hadjiyannakis, Stasia; Phillips, Penny; Alberga, Angela S.; Saunders, Travis J.; Tremblay, Mark S; Malcolm, Janine; Prud'homme, Denis; Gougeon, Rejeanne; Sigal, Ronald J.

    2011-01-01

    Objective To examine the association between duration and type of screen time (TV, video games, computer time) and blood pressure (BP) and lipids in overweight and obese adolescents. Design This is a cross-sectional study of 282 overweight or obese adolescents aged 14–18 years (86 males, 196 females) assessed at baseline prior to beginning a lifestyle intervention study for weight control. Sedentary behaviours, defined as hours per day spent watching TV, playing video games, recreational comp...

  8. Quality control of the blood pressure phenotype in the Gaoyou population study.

    Science.gov (United States)

    Lu, Yi-Chao; Li, Shan-Shan; Zhang, Hai-Feng; Odili, Augustine N; Yao, Wen-Ming; Gong, Lei; Zhou, Yan-Li; Zhou, Fang; Yang, Rong; Sheng, Yan-Hui; Xu, Dong-Jie; Kong, Xiang-Qing; Staessen, Jan A; Li, Xin-Li

    2016-06-01

    The Korotkoff approach is the only blood pressure (BP) measurement technique that allows contemporary data to be compared with decades of research. We randomly recruited 4483 people (53.3% women; mean age 52.1 years) from Gaoyou County, Jiangsu Province, China. Nine observers recorded the participants™ BP three times consecutively following Chinese Society of Hypertension guidelines. We assessed the BP phenotype based on five criteria: completeness of readings, percentage of identical BP readings, odd BP readings, end-digit preference and trends in BP from the first to the third reading. The proportion of participants with identical readings were 2.0% and 3.1% for systolic (SBP) and diastolic blood pressure (DBP), respectively. Among 26,898 BP values, 0.3% ended in an odd number. Among observers, the prevalence of identical readings varied from 0% to 5.3% for SBP and from 0% to 6.8% for DBP. Compared with the expected frequency of 20%, those ending in 0 had a lower frequency (17.2%; p < 0.001), whereas those ending in 8 had a higher frequency (22.4%; p < 0.001). From the first to the third measurement, SBP and DBP decreased (p < 0.001) by 0.87 and 0.55 mmHg, respectively. In conclusion, the procedures set up in the Gaoyou study produced a high-quality BP phenotype. PMID:26581308

  9. Blood antioxidant profile and lipid peroxides in dairy cows with clinical mastitis

    Directory of Open Access Journals (Sweden)

    Rajesh Rathore

    2013-10-01

    Full Text Available Aim: To evaluate blood antioxidant profile and lipid peroxides in dairy cows with clinical mastitis. Materials and Methods: Twelve cases of clinical mastitis in cross-bred cows were selected based on physical examination of udder and milk, California Mastitis Test (CMT, Somatic Cell Count (SCC and confirmation by bacteriological examination of milk and requisite biochemical tests. Twelve lactating cows showing negative CMT reaction and SCC <2x105 cells/ml were considered as healthy control. Antioxidant parameters measured in blood were superoxide dismutase (SOD, catalase activities and reduced glutathione (GSH concentration. Erythrocytic lipid peroxidation (LPO was measured in terms of malondialdehyde (MDA production. Results: Significant (P<0.05 decrease in blood SOD and catalase activities, GSH concentration and an increase in erythrocytic lipid peroxides was observed in cows with clinical mastitis. Conclusion: It is concluded that there is a compromise in antioxidant defense of the body in dairy cows with clinical mastitis resulting in oxidative damage, therefore, necessitate the use of antioxidants and other protective compounds along with conventional therapy for mastitis control. [Vet World 2013; 6(5.000: 271-273

  10. The Inflammatory Phenotype in Failed Metal-On-Metal Hip Arthroplasty Correlates with Blood Metal Concentrations.

    Directory of Open Access Journals (Sweden)

    Erja-Leena Paukkeri

    Full Text Available Hip arthroplasty is the standard treatment of a painful hip destruction. The use of modern metal-on-metal (MOM bearing surfaces gained popularity in total hip arthroplasties during the last decade. Recently, worrisome failures due to adverse reaction to metal debris (ARMD, including pseudotumor response, have been widely reported. However, the pathogenesis of this reaction remains poorly understood. The aim of the present study was to investigate the ARMD response by flow cytometry approach.Sixteen patients with a failed Articular Surface Replacement (ASR hip prosthesis were included in the study. Samples of pseudotumor tissues collected during revision surgery were degraded by enzyme digestion and cells were typed by flow cytometry. Whole blood chromium and cobalt concentrations were analyzed with mass spectrometry before revision surgery.Flow cytometry analysis showed that the peri-implant pseudotumor tissue expressed two principal phenotypes, namely macrophage-dominated and T-lymphocyte-dominated response; the average portions being 54% (macrophages and 25% (T-lymphocytes in macrophage-dominated inflammation and 20% (macrophages and 54% (T-lymphocytes in T-lymphocyte-dominated response. The percentages of B-lymphocytes and granulocytes were lower in both phenotypes. Interestingly, the levels of blood chromium and cobalt were significantly higher in patients with macrophage-dominated response.The results suggest that the adverse tissue reactions induced by MOM wear particles contain heterogeneous pathogeneses and that the metal levels are an important factor in the determination of the inflammatory phenotype. The present results support the hypothesis that higher metal levels cause cytotoxicity and tissue injury and macrophages are recruited to clear the necrotic debris. On the other hand, the adverse response developed in association with lower metal levels is T-lymphocyte-dominated and is likely to reflect hypersensitivity reaction.

  11. The Inflammatory Phenotype in Failed Metal-On-Metal Hip Arthroplasty Correlates with Blood Metal Concentrations

    Science.gov (United States)

    Paukkeri, Erja-Leena; Korhonen, Riku; Hämäläinen, Mari; Pesu, Marko; Eskelinen, Antti; Moilanen, Teemu; Moilanen, Eeva

    2016-01-01

    Introduction Hip arthroplasty is the standard treatment of a painful hip destruction. The use of modern metal-on-metal (MOM) bearing surfaces gained popularity in total hip arthroplasties during the last decade. Recently, worrisome failures due to adverse reaction to metal debris (ARMD), including pseudotumor response, have been widely reported. However, the pathogenesis of this reaction remains poorly understood. The aim of the present study was to investigate the ARMD response by flow cytometry approach. Methods Sixteen patients with a failed Articular Surface Replacement (ASR) hip prosthesis were included in the study. Samples of pseudotumor tissues collected during revision surgery were degraded by enzyme digestion and cells were typed by flow cytometry. Whole blood chromium and cobalt concentrations were analyzed with mass spectrometry before revision surgery. Results Flow cytometry analysis showed that the peri-implant pseudotumor tissue expressed two principal phenotypes, namely macrophage-dominated and T-lymphocyte-dominated response; the average portions being 54% (macrophages) and 25% (T-lymphocytes) in macrophage-dominated inflammation and 20% (macrophages) and 54% (T-lymphocytes) in T-lymphocyte-dominated response. The percentages of B-lymphocytes and granulocytes were lower in both phenotypes. Interestingly, the levels of blood chromium and cobalt were significantly higher in patients with macrophage-dominated response. Conclusions The results suggest that the adverse tissue reactions induced by MOM wear particles contain heterogeneous pathogeneses and that the metal levels are an important factor in the determination of the inflammatory phenotype. The present results support the hypothesis that higher metal levels cause cytotoxicity and tissue injury and macrophages are recruited to clear the necrotic debris. On the other hand, the adverse response developed in association with lower metal levels is T-lymphocyte-dominated and is likely to reflect

  12. Mesothelioma tumor cells modulate dendritic cell lipid content, phenotype and function.

    Directory of Open Access Journals (Sweden)

    Joanne K Gardner

    Full Text Available Dendritic cells (DCs play an important role in the generation of anti-cancer immune responses, however there is evidence that DCs in cancer patients are dysfunctional. Lipid accumulation driven by tumor-derived factors has recently been shown to contribute to DC dysfunction in several human cancers, but has not yet been examined in mesothelioma. This study investigated if mesothelioma tumor cells and/or their secreted factors promote increases in DC lipid content and modulate DC function. Human monocyte-derived DCs (MoDCs were exposed to human mesothelioma tumor cells and tumor-derived factors in the presence or absence of lipoproteins. The data showed that immature MoDCs exposed to mesothelioma cells or factors contained increased lipid levels relative to control DCs. Lipid accumulation was associated with reduced antigen processing ability (measured using a DQ OVA assay, upregulation of the co-stimulatory molecule, CD86, and production of the tolerogenic cytokine, IL-10. Increases in DC lipid content were further enhanced by co-exposure to mesothelioma-derived factors and triglyceride-rich lipoproteins, but not low-density lipoproteins. In vivo studies using a murine mesothelioma model showed that the lipid content of tumor-infiltrating CD4+ CD8α- DCs, CD4- CD8α- DCs DCs and plasmacytoid DCs increased with tumor progression. Moreover, increasing tumor burden was associated with reduced proliferation of tumor-antigen-specific CD8+ T cells in tumor-draining lymph nodes. This study shows that mesothelioma promotes DC lipid acquisition, which is associated with altered activation status and reduced capacity to process and present antigens, which may impair the ability of DCs to generate effective anti mesothelioma T cell responses.

  13. Heritability of eleven metabolic phenotypes in Danish and Chinese twins

    DEFF Research Database (Denmark)

    Li, Shuxia; Duan, Hongmei; Pang, Zengchang;

    2013-01-01

    A twin-based comparative study on the genetic influences in metabolic endophenotypes in two populations of substantial ethnic, environmental, and cultural differences was performed. Design and Methods: Data on 11 metabolic phenotypes including anthropometric measures, blood glucose, and lipids...

  14. Effects of Unsaturated Fat Dietary Supplements on Blood Lipids, and on Markers of Malnutrition and Inflammation in Hemodialysis Patients

    DEFF Research Database (Denmark)

    Ewers, Bettina; Riserus, Ulf; Marckmann, Peter

    2009-01-01

    OUTCOME MEASURES: Dietary intakes, blood lipids, dry body weight, serum albumin, and serum C-reactive protein comprised our main outcome measures. RESULTS: According to a per-protocol analysis of 14 study completers, fat supplementation resulted in significantly increased total energy intake (+1.6 MJ....../day, or 380kcal/day) and an increased dietary fat energy percentage (+9%). We observed no significant changes in blood lipids. Dry body weight (+0.49kg, P=.04) increased, and serum C-reactive protein concentration fell (-1.69mg/L, P=.01), with fat supplementation. Intention-to-treat analysis of 39...... participants confirmed the absence of adverse blood-lipid changes. CONCLUSIONS: Unsaturated fat supplementation increased total dietary energy intake to recommended levels, had no adverse impact on blood lipids, improved nutritional status as assessed according to dry body weight, and reduced systemic...

  15. Effect of Exercise on Blood Antioxidant Status and Erythrocyte Lipid Peroxidation: Role of Dietary Supplementation of Vitamin E

    OpenAIRE

    KELLE, Mustafa

    1999-01-01

    We tested the effects of moderate physical exercise on the blood antioxidant capacity and erythrocyte lipid peroxidation in 21 Wistar albino rats. Erythrocyte superoxide dismutase (SOD) activity increased significantly (p

  16. Blood F2-isoprostanes are significantly associated with abnormalities of lipid status in rats with steatosis

    Institute of Scientific and Technical Information of China (English)

    Man-Sie Zhu; Li-Juan Sun; Yue-Qin Uu; Yan-Ling Feng; Hai-Tao Tong; Ying-He Hu; Zheng Zhao

    2008-01-01

    AIM: To investigate oxidative stress and lipid peroxi-dation in hepatic steatosis and the underlying implica-tions in pathological mechanisms of non-alcoholic fatty liver disease (NAFLD).METHODS: F2-isoprostanes (iPF2α-Ⅲ) in blood and liver samples from steatotic (n = 9) and control (n = 7)rats were measured as in vivo marker of lipid peroxida-tion by a mass spectrometric approach. The lipid pro-file and endogenous antioxidant status (SOD and CAT)in the rats were also analyzed.RESULTS: Significantly higher levels of iPF2α-Ⅲ (mean3.47 vs 2.40 pmol/mg tissue, P = 0.004) and lower activities of SOD (mean 1.26 U vs 1.40 U, P < 0.001) and CAT (mean 1026.36 U/mg vs 1149.68 U/mg pro-tein, without significance) were observed in the livers of steatotic rats. Plasma total iPF2α-Ⅲ was significantly correlated with the abnormalities of blood lipids as well as alanine aminotransferase (ALT) levels in the rats with simple steatosis, whereas no similar tendencies were observed in the control rats.CONCLUSION: Enhancement of hepatic oxidative imbalance occurring at the steatotic stage of NAFLD suggests a possibility that manifestation of the local oxidative damage precedes that of systemic oxidativeimbalance. Predominant metabolic features of the in-creased lipid peroxidation further suggest a close asso-ciation of the oxidative imbalance and the dyslipidemia with functional deterioration of the steatotic liver. The findings need to be further evaluated, especially in hu-man studies.

  17. The Effects of 8-Weeks Aerobic Exercise Program on Blood Lipids and Cholesterol Profile of Smokers vs. Non Smokers

    Science.gov (United States)

    Taifour, Akef; AL-Shishani, Ahmad; Khasawneh, Aman; AL-Nawaiseh, Ali; Bakeer, Mohammed

    2015-01-01

    The aim of this study was to compare the effects of 8-week aerobic exercise program on blood lipids and cholesterol profile of smoker's vs. non-smokers. A total of 34 male subjects (18 non-smokers and 16 smokers) took part in this study. Both groups were pre- and post tested in their blood-lipids and cholesterol profile before and after the 8-week…

  18. Potential synergy between lipid-lowering and blood-pressure-lowering in the Anglo-Scandinavian Cardiac Outcomes Trial

    DEFF Research Database (Denmark)

    Sever, Peter; Dahlöf, Björn; Poulter, Neil; Wedel, Hans; Beevers, Gareth; Caulfield, Mark; Collins, Rory; Kjeldsen, Sverre; Kristinsson, Arni; McInnes, Gordon; Mehlsen, Jesper; Nieminem, Markku; O'Brien, Eoin; Ostergren, Jan

    2006-01-01

    A prespecified objective of the Anglo-Scandinavian Cardiac Outcomes Trial (ASCOT) was to assess whether any synergistic effects were apparent between the lipid-lowering and blood-pressure-lowering regimens in preventing cardiovascular events.......A prespecified objective of the Anglo-Scandinavian Cardiac Outcomes Trial (ASCOT) was to assess whether any synergistic effects were apparent between the lipid-lowering and blood-pressure-lowering regimens in preventing cardiovascular events....

  19. Effects of Mad Honey on Blood Glucose and Lipid Levels in Rats With Streptozocin-Induced Diabetes

    OpenAIRE

    ÖZTAŞAN, Nuray

    2005-01-01

    This study investigated whether mad honey affects blood glucose and lipid levels in experimental animals. It was found that mad honey caused significant decreases in blood glucose and lipid levels in animals with streptozocin-induced diabetes mellitus (n = 10) and controls (n = 10). These decreases may be due to grayanotoxins in the mad honey causing the islets of Langerhans in the pancreas to secrete insulin by stimulating the parasympathetic nervous system or M2-muscarinic receptors.

  20. Changes in the lipid composition of blood under the influence of a single submaximal exercise capacity (experimental research).

    OpenAIRE

    Ermolaeva E.N.

    2015-01-01

    In acute physical exercise, there is a change in oxygen delivery working tissues, blood gas transport function and efficiency of the use of oxygen by cells in the process of metabolism, which is the basis for compensation for physical activities. Lipid metabolism plays an important role in the energy supply of muscle activity. The aim of our research is to study the effect of a single submaximal exercise capacity by changing the lipid profile of peripheral blood. Materials and Methods. The st...

  1. Correlation bethealtyy ween dietary glycemic index and glycemic load and blood lipid levels in a group of women from Ahvaz

    OpenAIRE

    Farideh Shishebor; Zahra Shamekhi; Majid Karandish; Seyed Mahmood Latifi

    2011-01-01

    Background & Objectives: There are limited number of studies conducted on the correlation between Glycemic index and Glycemic load of a food program and metabolic factors such as blood lipids in Asian countries including Iran. Therefore, this study aimed at analyzing the correlation between Glycemic index and Glycemic load of Iranian food program and blood lipids. Materials & Methods: The subjects were 95 women working in Ahvaz University of Medical Sciences in the range of 20 to 55 years old...

  2. Estimating the future burden of cardiovascular disease and the value of lipid and blood pressure control therapies in China

    OpenAIRE

    Stevens, Warren; Peneva, Desi; Li, Jim Z; Liu, Larry Z.; Liu, Gordon; Gao, Runlin; Lakdawalla, Darius N.

    2016-01-01

    Background Lifestyle and dietary changes reflect an ongoing epidemiological transition in China, with cardiovascular disease (CVD) playing an ever-increasing role in China’s disease burden. This study assessed the burden of CVD and the potential value of lipid and blood pressure control strategies in China. Methods We estimated the likely burden of CVD between 2016 and 2030 and how expanded use of lipid lowering and blood pressure control medication would impact that burden in the next 15 yea...

  3. The effects of three-week fasting diet on blood pressure, lipid profile and glucoregulation in extremely obese patients

    OpenAIRE

    Beleslin Biljana; Ćirić Jasmina; Žarković Miloš; Penezić Zorana; Vujović Svetlana; Trbojević Božo; Drezgić Milka

    2007-01-01

    Introduction Obesity is often accompanied by a number of complications including diabetes mellitus and cardiovascular diseases. Elevated blood pressure and lipids, as well as deterioration of glucoregulation are attributed, as the most significant factors, to development of diabetes mellitus and cardiovascular complications in obese patients. Objective The aim of our study was to evaluate the effects of a fasting diet on blood pressure, lipid profile and glucoregulatory parameters. Method We ...

  4. Phenotypic and functional characteristics of dendritic cells derived from human peripheral blood monocytes

    Institute of Scientific and Technical Information of China (English)

    TANG Ling-ling; ZHANG Zhe; ZHENG Jie-sheng; SHENG Ji-fang; LIU Ke-zhou

    2005-01-01

    Objective: This study is aimed at developing a simple and easy way to generate dendritic cells (DCs) from human peripheral blood monocytes (PBMCs) in vitro. Methods: PBMCs were isolated directly from white blood cell rather than whole blood and purified by patching methods (collecting the attached cell and removing the suspension cell). DCs were then generated by culturing PBMCs for six days with 30 ng/ml recombinant human granulocyte-macrophage stimulating factor (rhGM-CSF) and 20 ng/ml recombinant human interleukin-4 (rhIL-4) in vitro. On the sixth day, TNF-alpha (TNFα) 30 ng/ml was added into some DC cultures, which were then incubated for two additional days. The morphology was monitored by light microscopy and transmission electronic microscopy, and the phenotypes were determined by flow cytometry. Autologous mixed leukocyte reactions (MLR) were used to characterize DC function after TNFα or lipopolysaccharide (LPS) stimulations for 24 h. Results: After six days of culture, the monocytes developed significant dendritic morphology and a portion of cells expressed CD 1 a, CD80 and CD86, features of DCs. TNFα treatment induced DCs maturation and up-regulation of CD80, CD86 and CD83. Autologous MLR demonstrated that these DCs possess potent T-cell stimulatory capacity. Conclusion: This study developed a simple and easy way to generate DCs from PBMCs exposed to rhGM-CSF and rhIL-4. The DCs produced by this method acquired morphologic and antigenic characteristics of DCs.

  5. Metabolic phenotyping by 1H-NMR spectroscopy to detect lung cancer via a simple blood sample

    OpenAIRE

    Louis,Evelyne; Adriaensens, Peter; MESOTTEN, Liesbet; Thomeer, Michiel; Reekmans, Gunter; Vanhove, Karolien; Vandeurzen, Kurt; Darquennes, Karen

    2013-01-01

    Introduction: Lung cancer is the leading cause of cancer death worldwide. There is an urgent need of effective methods to detect lung cancer. Accumulating evidence shows that the metabolism of cancer cells differs from that of normal cells. Disturbances in biochemical pathways which occur during the development of cancer provoke changes in the metabolic phenotype. Objective: To determine the metabolic phenotype of lung cancer by 1H-NMR spectroscopy. Methods: Fasting venous blood samples of 78...

  6. Phenotype frequencies of blood group systems (Rh, Kell, Kidd, Duffy, MNS, P, Lewis, and Lutheran in blood donors of south Gujarat, India

    Directory of Open Access Journals (Sweden)

    Manoj A Kahar

    2014-01-01

    Full Text Available Background: This is the first study on phenotype frequencies of various blood group systems in blood donors of south Gujarat, India using conventional tube technique. Material and Methods: A total of 115 "O" blood group donors from three different blood banks of south Gujarat were typed for D, C, c, E, e, K, Jk a , Le a , Le b , P 1 , M, and N antigens using monoclonal antisera and k, Kp a , Kp b , Fy a ,Fy b , Jk b , S,s, Lu a , and Lu b antigens were typed using polyclonal antisera employing Indirect Antiglobulin Test. Antigens and phenotype frequencies were expressed as percentages. Results: From the 115 blood donor samples used for extended antigen typing in the Rh system, e antigen was found in 100% donors, followed by D [84.35%], C [81.74%], c [56.32%], and E [21.74%] with DCe/DCe (R 1 R 1 , 40.87% as the most common phenotype. k was found to be positive in 100% of donors and no K+k- phenotype was found in Kell system. For Kidd and Duffy blood group system, Jk(a+b+ and Fy(a-b- were the most common phenotypes with frequency of 52.17% and 48.69%, respectively. In the MNS system, 39.13% donors were typed as M+N+, 37.39% as M+N-, and 23.48% as M-N+. S+s+ was found in 24.35% of donors, S+s- in 8.69%, and S-s+ as the commonest amongst donors with 66.96%. No Lu(a+b+ or Lu(a+b- phenotypes were detected in 115 donors typed for Lutheran antigens. A rare Lu(a-b- phenotype was found in 2.61% donors. Conclusion: Data base for antigen frequency of various blood group systems in local donors help provide antigen negative compatible blood units to patients with multiple antibodies in order to formulate in-house red cells for antibody detection and identification and for preparing donor registry for rare blood groups.

  7. Lipid spectrum of the newborn rats' blood at the radioactive and chemical effects in the prenatal period

    International Nuclear Information System (INIS)

    The radioactive and chemical factors used in complex or separately during the prenatal period in the experiment induce ambiguous effects on the lipid metabolism in blood plasma and erythrocytes of newborn rats. The chemicals cause more significant changes in the blood plasma lipid metabolism than the radioactive irradiation does. Being used combined the radioactive and chemical factors do not increase each other's effect- their effects have opposite directions. The radiochemical exposure induce more significant shifts in the lipid spectrum in erythrocytic membranes than the separate factors

  8. Effect of homocysteine-lowering nutrients on blood lipids: results from four randomised, placebo-controlled studies in healthy humans.

    Directory of Open Access Journals (Sweden)

    Margreet R Olthof

    2005-05-01

    Full Text Available BACKGROUND: Betaine (trimethylglycine lowers plasma homocysteine, a possible risk factor for cardiovascular disease. However, studies in renal patients and in obese individuals who are on a weight-loss diet suggest that betaine supplementation raises blood cholesterol; data in healthy individuals are lacking. Such an effect on cholesterol would counteract any favourable effect on homocysteine. We therefore investigated the effect of betaine, of its precursor choline in the form of phosphatidylcholine, and of the classical homocysteine-lowering vitamin folic acid on blood lipid concentrations in healthy humans. METHODS AND FINDINGS: We measured blood lipids in four placebo-controlled, randomised intervention studies that examined the effect of betaine (three studies, n = 151, folic acid (two studies, n = 75, and phosphatidylcholine (one study, n = 26 on plasma homocysteine concentrations. We combined blood lipid data from the individual studies and calculated a weighted mean change in blood lipid concentrations relative to placebo. Betaine supplementation (6 g/d for 6 wk increased blood LDL cholesterol concentrations by 0.36 mmol/l (95% confidence interval: 0.25-0.46, and triacylglycerol concentrations by 0.14 mmol/l (0.04-0.23 relative to placebo. The ratio of total to HDL cholesterol increased by 0.23 (0.14-0.32. Concentrations of HDL cholesterol were not affected. Doses of betaine lower than 6 g/d also raised LDL cholesterol, but these changes were not statistically significant. Further, the effect of betaine on LDL cholesterol was already evident after 2 wk of intervention. Phosphatidylcholine supplementation (providing approximately 2.6 g/d of choline for 2 wk increased triacylglycerol concentrations by 0.14 mmol/l (0.06-0.21, but did not affect cholesterol concentrations. Folic acid supplementation (0.8 mg/d had no effect on lipid concentrations. CONCLUSIONS: Betaine supplementation increased blood LDL cholesterol and triacylglycerol

  9. Camphor Tree Seed Kernel Oil Reduces Body Fat Deposition and Improves Blood Lipids in Rats.

    Science.gov (United States)

    Fu, Jing; Wang, Baogui; Gong, Deming; Zeng, Cheng; Jiang, Yihao; Zeng, Zheling

    2015-08-01

    The total and positional fatty acid composition in camphor tree (Cinnamomum camphora) seed kernel oil (CKO) were analyzed, and for the first time, the effect of CKO on body fat deposition and blood lipids in rats was studied. The major fatty acids in CKO were determined to be decanoic acid (C10:0, 51.49%) and dodecanoic acid (C12:0, 40.08%), and uniformly distributed at Sn-1, 3, and Sn-2 positions in triglyceride (TG). Rats were randomly divided into control, CKO, lard, and soybean oil groups. At the end of the experiment, levels of blood lipids and the fats of abdomen in the rats were measured. The main organ were weighted and used for the histological examination. The results showed that body weight and fat deposition in CKO group were significantly lower than the lard and soybean groups. Moderate consumption of CKO was found to improve the levels of blood TG and low density lipoprotein cholesterol. PMID:26130050

  10. Study on the relationship between red blood cell immunity and lipid peroxidation in patients with endometriosis

    International Nuclear Information System (INIS)

    Objective: To assess the relationship between red blood cell immunity and lipid peroxidation (LPO) in patients with endometriosis. Methods: The percentage of positive red blood cell c3b receptor rosette (RBCc3b-RR) and red blood cell immune complex rosette (RBC-ICR) were examined in 54 patients with endometriosis and 30 controls. Serum levels of malondialdehyde (MDA), superoxidase (SOD) and glutathione peroxidase (GSH-PX) were measured by chemocolorimetry in these subjects. Results: Percentage of positive RBC-ICR and MDA levels were significantly higher in patients with endometriosis than those in controls (Pc3bRR, SOD, GSH-PX, SOD/MDA ratio were significantly lower in patients with endometriosis than those in controls (Pc3b-RR was negatively correlated with MDA levels (r= -0. 4428, P < 0.05) and RBC-ICRR was positively correlated with MDA(r=0.5488, P0.05). Conclusion: The lower red cell immune adhesion function was closely associated with the disturbance of metabolism of lipid peroxidation in patients with endometriosis. (authors)

  11. Salivary latent trait cortisol (LTC): Relation to lipids, blood pressure, and body composition in middle childhood.

    Science.gov (United States)

    Yeung, Ellen W; Place, Rebecca; Gordish-Dressman, Heather; Visich, Paul; Hoffman, Eric; Walker, Sheila O; Granger, Douglas A

    2016-09-01

    Adversity experienced early in life has the potential to influence physical health later in life. The stress-health relation may be partially explained by stress-related effects on cardiovascular risk factors. This study explored links between individual differences in trait-like variation in the activity of the hypothalamic-pituitary-adrenal (HPA) axis with cardiovascular risk factors in children. 474 children (M age=9.22years; 54% female; 83% Caucasian) were included in this study, in which cardiovascular risk was assessed using the following indices - triglycerides (TG), HDL-cholesterol (HDL-C), glucose (Glu); resting systolic and diastolic blood pressure, body mass index (BMI), waist-to-hip ratio, and % fat. Saliva samples were measured 3 times a day (waking, 30min post-waking and bedtime) over 3days (later assayed for cortisol). A latent trait cortisol (LTC) factor explained 43% of the variance in cortisol levels within and across days. Confirmatory factor analysis identified three cardiovascular risk factors: lipids (i.e., TG and HDL-C), blood pressure (i.e., systolic and diastolic), and body composition (i.e., BMI, Waist-to-hip ratio, and % fat). Lower salivary LTC was associated with higher lipids, higher blood pressure, and higher body composition. The findings further support the internal and external validity of the LTC construct, and may also advance our understanding of the link between interindividual differences in HPA axis activity and cardiovascular risk in middle childhood. PMID:27262343

  12. GUAR GUM EFFECTS ON BLOOD SERUM LIPIDS AND GLUCOSE CONCENTRATIONS OF WISTAR DIABETIC RATS1

    Directory of Open Access Journals (Sweden)

    DARIO FRIAS Andrea

    1998-01-01

    Full Text Available The effects of guar gum derived from the endosperm of Cyamopsis tetragonoloba (75% soluble fiber, 7.6% insoluble fiber, 2.16% crude protein, 0.78% total lipids, 0.54% ash and 9.55% moisture on diabetic rats were studied concerning food intake, body weight gain, blood serum cholesterol, triacylglycerols, glucose, LDL-, and HDL-cholesterol concentrations. The effect of gum on indexes of protein absorption and utilization was also investigated. Diets containing 0%, 10% and 20% (w/w guar gum were fed to diabetic rats for 28 days. In spite of the fact that diabetes elevated blood lipids in all animals, guar gum diet significantly decreased (p <0.05 serum concentrations of cholesterol and triacylglycerols. Furthermore, a concomitant increase in HDL-cholesterol with a substancial elevation of the HDL/LDL cholesterol ratio was found. The most significant result in this assay was the drastic reduction of blood glucose in diabetic rats treated with guar gum diet. The gum promoted a general improvement in the condition of the diabetic rats, in body weight gain and indexes of protein absorption and utilization. The results of this research suggest that guar gum, at concentrations equal to or higher than 10%, should be effective in the treatment of hypercholesterolemia and diabetes, in humans.

  13. Removal of. cap alpha. -tocopherol from blood and its comparison with other lipids: studies of inhibition

    Energy Technology Data Exchange (ETDEWEB)

    Gardner, H.K.; Mavis, R.D.

    1986-05-01

    To investigate the mechanism of ..cap alpha..-tocopherol (AT) uptake into tissues, loss of /sup 3/H-AT from blood was characterized and compared with the losses of two major blood lipids: /sup 3/H-cholesterol (CHO) and /sup 3/H-trioleoylglycerol (TO). Male Long-Evans rats (200-325 gm) were injected with serum labelled with one lipid, and bled from the tail from 1-240 min. In one group heparin (HEP), an inhibitor of lipoprotein lipase which mediates uptake of TO into tissues, was intravenously injected prior to serum and following corn oil gavage. The control group was only gavaged (GAV). A third group was injected with labelled serum which had been incubated with 1,2-cyclohexanedione (CHD), a reagent which modifies the receptors responsible for removal of CHO-rich low density lipoproteins from blood. Labelled serum incubated only with borate buffer (BO) was injected into the fourth group. HEP slowed TO loss from 2-220 min, but left CHO loss unchanged. AT loss was slowed by HEP from 100 min on. That AT responded to HEP but over a time span different from that of TO suggests that AT may be removed by a mechanism distinct from that of TO but sensitive to HEP. CHD slowed CHO loss from 40-240 min while TO and AT loss were uninhibited. This argues against a mechanism of removal common to both AT and CHO.

  14. Acupoint Application on Blood Lipid in Patients with Type Ⅱ Diabetes

    Institute of Scientific and Technical Information of China (English)

    SHAO Min; WEN Ling-jie; SUN Rong

    2004-01-01

    Purpose: To observe the regulative effect of acupoint application of abnormai lipid metabolism in type Ⅱ diabetes. Methods: Thirty patients with type Ⅱ diabetes were treated by acupoint application and routine hypoglycemic agents. Meanwhile, another 30 patients with type Ⅱ diabetes were treated by routine hypoglycemic agents only. A difference in the blood-lipid level between pretreatment and post-treatment was observed in the two groups. Results: Differences in cholesterol,triglyceride, low-density lipoprotein and high-density lipoprotein between pre-treatment and posttreatment in treatment group were significantly different from those in control group(P<0.01).Conclusion: Acupoint application has a good lowering effect on high blood lipid and can effectively alleviate the disorder of lipid metabolism in diabetics.%目的:观察穴位敷贴对调整Ⅱ型糖尿病脂类代谢异常的作用.方法:应用穴位敷贴加常规降糖药治疗30例Ⅱ型糖尿病患者,同时单用常规降糖药治疗30例Ⅱ型糖尿病患者,观察治疗前后两组血脂水平的变化.结果:治疗组与对照组比较,胆固醇、甘油三酯、低密度脂蛋白和高密度脂蛋白治疗前后差值,有显著性意义(P<0.01).结论:穴位敷贴对降低糖尿病患者高血脂有良好的作用,能有效改善患者脂代谢紊乱的状况.

  15. Effects of soy components on blood and liver lipids in rats fed high-cholesterol diets

    Institute of Scientific and Technical Information of China (English)

    Ching-Yi Lin; Cheng-Yu Tsai; Shyh-Hsiang Lin

    2005-01-01

    AIM: To assess the effects of soy protein, isoflavone, and saponin on liver and blood lipid in rats that consumed high-cholesterol diets.METHODS: High-cholesterol diets (1%) with or without soy material were fed to 6-wk-old male Sprague-Dawley rats for 8 wk. Blood lipids, liver lipids, glutamic oxaloacetic transaminase (GOT), and glutamic pyruvic transaminase (GPT) levels were measured. The in vitro bile acid-binding ability of soy materials was analyzed.RESULTS: The results of in vitro studies showed that soy protein isolate had a significantly higher bile acid-binding ability (8.4±0.8%) than soy saponin (3.1±0.7%) and isoflavone (1.3±0.4%, P<0.05). On the other hand, at the end of the experimental period, rats that consumed soy protein diets had lower GOT and GPT levels than rats that consumed casein under high-cholesterol diets.Rats that consumed soy protein also had lower total cholesterol (TC) levels in the liver than those that consumed casein under high-cholesterol diets. Rats that consumed the soy protein diet containing both saponin and isoflavone had lower hepatic TC level than those that consumed the soy protein diet without isoflavone alone.The effect of different types of proteins on triglyceride was not significant.CONCLUSION: Consumption of soy provided benefits to control lipid levels under high-cholesterol dieting conditions in this rat model of hypercholesterolemia. The major component that reduced hepatic TC was not saponin, but possibly isoflavone.

  16. Video game playing is independently associated with blood pressure and lipids in overweight and obese adolescents.

    Directory of Open Access Journals (Sweden)

    Gary S Goldfield

    Full Text Available OBJECTIVE: To examine the association between duration and type of screen time (TV, video games, computer time and blood pressure (BP and lipids in overweight and obese adolescents. DESIGN: This is a cross-sectional study of 282 overweight or obese adolescents aged 14-18 years (86 males, 196 females assessed at baseline prior to beginning a lifestyle intervention study for weight control. Sedentary behaviours, defined as hours per day spent watching TV, playing video games, recreational computer use and total screen time were measured by self-report. We examined the associations between sedentary behaviours and BP and lipids using multiple linear regression. RESULTS: Seated video gaming was the only sedentary behaviour associated with elevated BP and lipids before and after adjustment for age, sex, pubertal stage, parental education, body mass index (BMI, caloric intake, percent intake in dietary fat, physical activity (PA duration, and PA intensity. Specifically, video gaming remained positively associated with systolic BP (adjusted r = 0.13, β = 1.1, p<0.05 and total cholesterol/HDL ratio (adjusted r = 0.12, β = 0.14, p<0.05. CONCLUSIONS: Playing video games was the only form of sedentary behaviour that was independently associated with increased BP and lipids. Our findings provide support for reducing time spent playing seated video games as a possible means to promote health and prevent the incidence of cardiovascular disease (CVD risk factors in this high risk group of overweight and obese adolescents. Future research is needed to first replicate these findings and subsequently aim to elucidate the mechanisms linking seated video gaming and elevated BP and lipids in this high risk population. TRIAL REGISTRATION: Clinicaltrials.gov NCT00195858.

  17. Proof of concept in cardiovascular risk: the paradoxical findings in blood pressure and lipid abnormalities.

    Science.gov (United States)

    Fuchs, Flavio Danni; Fuchs, Sandra Costa; Moreira, Leila Beltrami; Gus, Miguel

    2012-01-01

    High blood pressure and lipoprotein abnormalities were identified by many cohort studies as the major risk factors for cardiovascular disease. Laboratory experiments apparently confirmed their role in the causation of atherosclerosis, but a proof of concept requires the corroboration by clinical trials in human beings. The size of benefit in clinical trials regarding the control of high blood pressure was within the estimations of risk provided by cohort studies. For a reduction of 10 mmHg in systolic blood pressure or 5 mmHg in diastolic blood pressure, the relative risk reduction of coronary heart disease was 22% (95% confidence interval 27%-17%) in a meta-analysis of clinical trials, close to the estimation of reduction of 25% (95% confidence interval 23%-27%) provided by a meta-analysis of cohort studies. The corresponding values for stroke were 41% (95% confidence interval 33%-48%) in clinical trials compared to a cohort risk prediction of 36% (95% confidence interval 34%-38%). This efficacy was shared by all blood pressure-lowering drugs. The same figure has not paradoxically happened with drugs that act over abnormalities of cholesterol and lipoproteins. Only statins, which have other beneficial actions as well, have consistently lowered the incidence of cardiovascular diseases, an efficacy that was not reproduced by older and newer quite potent lipid drugs. The adverse effects of these drugs may nullify their beneficial effects over lipoproteins and abnormalities of lipoproteins may only be surrogate markers of the underlying real risks. PMID:22870036

  18. Association between Dietary Patterns and Blood Lipid Profiles in Korean Adults with Type 2 Diabetes

    OpenAIRE

    Lim, Jeong Hyun; Lee, Yeon-Sook; Chang, Hak Chul; Moon, Min Kyong; Song, YoonJu

    2011-01-01

    We aimed to explore the associations of dietary patterns with blood lipid profiles and obesity in adults with type 2 diabetes. The data were obtained from the Forth Korean National Health and Nutrition Examination Survey, 2007-2008. Adults 30 yr or older, from which had both biochemical and dietary data were obtained. Among them, 680 subjects were defined as having diabetes based on criteria of fasting glucose ≥ 126 mg/dL, anti-diabetic treatment, or previously diagnosed diabetes. Dietary dat...

  19. Inadequate physician knowledge of the effects of diet on blood lipids and lipoproteins.

    Directory of Open Access Journals (Sweden)

    Sciamanna Christopher

    2003-12-01

    Full Text Available Abstract Background To assess the nutrition knowledge of physicians on the basic effects of diet on blood lipids and lipoproteins. Methods Anonymous mailed dietary knowledge surveys to 6000 randomly selected physicians in the United States licensed in either Internal Medicine or Cardiology. Results Response rate: 16% (n = 639. Half of the physicians did not know that canola oil and 26% did not know olive oil were good sources of monounsaturated fat. Ninety-three percent (84% of cardiologists vs. 96% of internists; p Conclusions If physicians are to implement dietary and cholesterol management guidelines, they will likely need to become more knowledgeable about nutrition.

  20. Effects of medium-chain fatty acids and oleic acid on blood lipids, lipoproteins, glucose, insulin, and lipid transfer protein activities

    DEFF Research Database (Denmark)

    Tholstrup, T.; Ehnholm, C.; Jauhiainen, M.;

    2004-01-01

    cholesterol, although this claim is poorly documented. Objective: We compared the effects of a diet rich in either MCFAs or oleic acid on fasting blood lipids, lipoproteins, glucose, insulin, and lipid transfer protein activities in healthy men. Design: In a study with a double-blind, randomized, crossover...... design, 17 healthy young men replaced part of their habitual dietary fat intake with 70 g MCTs (66% 8:0 and 34% 10:0) or high-oleic sunflower oil (89.4% 18:1). Each intervention period lasted 21 d, and the 2 periods were separated by a washout period of 2 wk. Blood samples were taken before and after the...... oleic acid, MCT fat unfavorably affected lipid profiles in healthy young men by increasing plasma LDL cholesterol and triacylglycerol. No changes in the activities of phospholipid transfer protein and cholesterol ester transfer protein were evident....

  1. Blood pressure, heart rate and lipids in professional handball and water polo players

    Directory of Open Access Journals (Sweden)

    Jovanović Jovica

    2005-01-01

    Full Text Available Introduction: Blood pressure, heart rate and lipoprotein lipids are affected by family history, obesity, diet, smoking and physical activity habits. The aim of this paper was to estimate the values of blood pressure and heart rate in professional handball and water polo players before and after training and submaximal exercise test and to analyze the lipid state in these professional athletes in comparison with people who have never been in sports. Material and methods The investigation included 30 professional handball players, 30 professional water polo players and 15 men who have never been in sports (control group. All groups were matched for age, smoking habits, family predisposition to arterial hypertension and dyslipidemia. Results Between these groups there were statistically significant differences of blood pressure values and heart rate in the state of rest, after exercise test and after the training. There were also statistically significant differences of total cholesterol values, LDL cholesterol, triglycerides, HDL cholesterol, LDL cholesterol/HDL cholesterol ratio and total cholesterol/HDL ratio between these groups. Discussion Differences between these groups can be explained by various values of body mass index, by activity of lipoprotein lipase in athletes, by body position during the sports activity, by thermoregulatory vasoconstriction in the water, and by effects of hydrostatic pressure and reflex mechanisms during swimming. Conclusions Cardiovascular reaction in professional athletes depends on the type of sports activity, body position and medium during training. Professional athletes have a lower atherogenic risk than non-sportsmen. Changes of blood pressure and heart rate after submaximal exercise test are not the same as changes after training. .

  2. Study of the Effect of Garlic on Serum Lipids and Blood Glucose Levels in Type 2 Diabetic Patients

    OpenAIRE

    M Afkhami - Ardekani; AR Kamali - Ardakani

    2005-01-01

    Introduction: Hyperlipidemia and diabetes are common risk factors for ischemic heart disease, which is the main cause of mortality in diabetic patients. Strict control of blood glucose and other risk factors in diabetics has led to prevention of complications. Garlic has received particular attention for control of blood glucose and decrease in blood lipid levels. At present, several studies have been carried out in order to prove advantages of garlic. Methods: In this study, effects of garsi...

  3. Effects of osthol on blood pressure and lipid metabolism in stroke-prone spontaneously hypertensive rats.

    Science.gov (United States)

    Ogawa, Hiroshi; Sasai, Noriko; Kamisako, Toshinori; Baba, Kimiye

    2007-05-30

    Osthol, a coumarin compound, was isolated from the dried fruits of Cnidium monnieri (Umbelliferae) and the effect of dietary osthol on hypertension and lipid metabolism was examined in stroke-prone spontaneously hypertensive rats (SHRSP). Six-week-old male SHRSP were fed the experimental diet containing 0.05% osthol by weight for 4 weeks with free access to the diet and water. Elevation of systolic blood pressure was significantly suppressed on and after 3 weeks. In addition, significant decreases in cholesterol and triglyceride contents in the liver were recognized without any significant changes in serum lipids profiles. A comparative study on hepatic mRNA expression indicated that osthol induced a significant increase in 3-hydroxy-3-methylglutaryl coenzymeA (HMG-CoA) reductase mRNA expression, which may lead to decrease in hepatic cholesterol pool through inhibition of the enzyme activity. Moreover, osthol induced a significant increase in acyl-CoA oxidase mRNA expression associated with an increase in carnitine palmitoyl transferase 1a mRNA expression, which suggests the acceleration of beta-oxidation of hepatic fatty acids. This may be responsible, at least in part, for the reduction of hepatic triglyceride content in SHRSP. These beneficial effects of osthol could be useful for both prevention of atherosclerosis and suppression of hepatic lipid accumulation. PMID:17324541

  4. Systematic Review of Chinese Traditional Exercise Baduanjin Modulating the Blood Lipid Metabolism

    Directory of Open Access Journals (Sweden)

    Lijuan Mei

    2012-01-01

    Full Text Available Background. Baduanjin exercise is considered to be beneficial to modulate the blood lipid metabolism. The purpose of the systematic review was to assess the potential efficacy and safety of Baduanjin exercise. Methods. MEDLINE, EMBASE, CBM, CNKI, VIP, Chinese Important Conference Papers Database, and Chinese Dissertation Database were searched for all prospective-controlled trials of Baduanjin exercise from their inception to December 31, 2011. Results. A total of 14 studies were included. Comparing with no treatment, Baduanjin exercise significantly reduced the levels of TC, TG, LDL-C in plasma, and elevated plasma HDL-C level for healthy participants, and the pooled MD (95% confidence interval, CI was −0.58 mmol/L (−0.86, −0.30 mmol/L, −0.22 mmol/L (−0.31, −0.13 mmol/L, −0.35 mmol/L (−0.54, −0.17 mmol/L, 0.13 mmol/L (0.06, 0.21 mmol/L, respectively. Baduanjin exercise also obviously decreased the levels of TG, LDL-C in plasma comparing with no treatment for patients, and the pooled MD (95% CI was −0.30 mmol/L (−0.40, −0.19 mmol/L, −0.38 mmol/L (−0.63, −0.13 mmol/L, but there was not obvious to decrease plasma TC level or elevate plasma HDL-C level in patients with the pooled MD (95%CI, −0.39 mmol/L (−1.09, 0.31 mmol/L and 0.22 mmol/L (−0.11, 0.55 mmol/L, respectively. In addition, the obvious advantage was not observed to modulate the blood lipid metabolism in comparing Baduanjin exercise with other exercises, regardless for health participants or patients. Conclusion. Studies indicated that Baduanjin exercise could significantly decrease the levels of TC, TG, LDL-C levels in plasma and elevate plasma HDL-C level for the healthy people. It also was helpful that Baduanjin exercise modulated the blood lipid metabolism for patients. Moreover, the Baduanjin exercise did not have an obvious advantage on modulating the lipid metabolism comparing with other exercises. But the

  5. The relationship between serum lipid levels, high blood pressure and obesity in children

    Directory of Open Access Journals (Sweden)

    Meltem Kurtuncu

    2014-03-01

    Full Text Available Objective: The purpose of this study was to determine serum lipid, cholesterol and obesity levels in healthy children, and then to explore the relationships between these factors. Methods: The sample group consisted of 103 students at Gelik Elementary School in Zonguldak, Turkey who was willing to participate in the study, had not been medically diagnosed with a chronic ailment, and were not taking regular medications. Results: When the students' mean BMI (Body Mass Index measurements were considered, it was found that mean BMI at 13 years of age (21.03 ± 0.23 was higher than at 14 years (20.05 ± 0.20 and that this difference was strongly significant (p=0.002. When the relationships between the students' height, weight, BMI, blood pressure, FBS (Fasting Blood Sugar, cholesterol, LDL (Low Density Lipoprotein, HDL (High Density Lipoprotein measurements were considered, it was seen that there was a positive significant relationship between height-weight (r=0.472; p<0.001, height-blood pressure (r=0.432; p<0.001, and height-FBS (r=0.332; p=0.001. Conclusions: The conclusion drawn was that monitoring blood pressure, cholesterol, LDL and HDL levels in childhood medical examinations is not only important in terms of identifying obesity, but also in identifying nutritional mistakes and deficiencies at an early stage.

  6. Analysis of relationship between blood lipid metabolism levels and hs-CRP levels in patients with chronic obstructive pulmonary disease

    International Nuclear Information System (INIS)

    Objective: To study the relationship between blood lipid metabolism levels and hs-CRP levels in the patients with chronic obstructive pulmonary disease. Methods: The levels of plasma blood lipid (with biochemistry) and serum hs-CRP(with high-sensitive immuno turbidimetry) were determined in 96 patients with chronic obstructive pulmonary disease as well as 68 normal controls. Results: The plasma blood lipid levels in 96 patients with chronic obstructive pulmonary disease were significantly lower than those in 68 controls, plasma TC and LDL-C levels were not much difference (P>0.05), plasma HDL-C level was significantly difference (P<0.05), but TG and Lp (a) levels were very prominently difference (P<0.01). And the plasma hs-CRP level was significantly increased also (P<0.01). The close relationship was between blood lipid and hs-CRP levels. Conclusion: The study of relationship between blood lipid levels and hs-CRP levels in patients with COPD was helpful for understand the disease process as well as possible mechanisms. (authors)

  7. Investigation the effect of Commiphora mukul on blood glucose and Serum lipid profile in diabetic rats

    Directory of Open Access Journals (Sweden)

    leila Shirazi

    2015-01-01

    Full Text Available Background & aim: The decrease of serum glucose level and lipids in diabetic patients is clinically significant. The purpose of this study was to determine the effect of Commiphora mukul extract on blood sugar and lipid profile in diabetic rats. Methods: In the present experimental study, thirty-two male Wistar rats were randomly divided into four groups: control, control treated with the extract, diabetic and diabetic treated with the extract. Diabetes was induced by intraperitoneal injection of Streptozotocin. In an eight week period, the control group of normal saline and the control group and diabetic recipient extract of CM oleo gum blue resin was given by gavage. Treatment resumed eight weeks with onset of hyperglycemia. The control and diabetic control groups received normal saline orally. Extract treated control and extract treated diabetic groups received extract of Commiphora mukul gum (300 mg/kg P.O. daily by gavage. At the end of this period, blood samples were collected from each rat and biochemical tests for investigation of glucose level and lipid profile was performed. One- way analysis of variance (ANOVA statistical test and Post-hoc test Tukey’s were used for data analysis Results: The study indicated that diabetes increases the serum levels of glucose, cholesterol, triglycerides, LDL and HDL. Administration of Commiphora mukul gum extract in diabetic groups significantly decreased the serum level of glucose, cholesterol(p<0.01 and triglyceride and LDL(p<0.001 and increased HDL(p<0.01. Conclusion: Commiphora mukul gum extract may well improve undesirable effects of diabetes on serum level of glucose, cholesterol, triglyceride, LDL and HDL.

  8. Influence of a hyperlipidic diet on the composition of the non-membrane lipid pool of red blood cells of male and female rats.

    OpenAIRE

    Remesar, Xavier; Antelo, Arantxa; Llivina, Clàudia; Albà, Emma; Berdié, Lourdes; Agnelli, Silvia; Arriarán, Sofía; Fernández-López, José Antonio; Alemany, Marià

    2015-01-01

    Background and objectives. Red blood cells (RBC) are continuously exposed to oxidative agents, affecting their membrane lipid function. However, the amount of lipid in RBCs is higher than the lipids of the cell membrane, and includes triacylglycerols, which are no membrane components. We assumed that the extra lipids originated from lipoproteins attached to the cell surface, and we intended to analyse whether the size and composition of this lipid pool were affected by sex or diet. Experiment...

  9. Effects of Soybean Fibers on Blood Suga,Lipid Levels and Hepatic_nephritic Histomorphology in Mice With Diabetes Mellitus

    Institute of Scientific and Technical Information of China (English)

    XUHONG; TANSHU-MEI; 等

    2001-01-01

    Objective:To study the influence of soybean fibers on blood sugar,blood-lipid metabolism,and histomorphology in mice odels with diabetes mellitus(D).Method:After normal mice and mice with Streptozocin(STZ)-induced DM were fed with forage containing a certain amount of soybean fiber(SF) for 5 weeks,blood sugar and blood lipid were determined,and morphological changes of hepatic and nephritic tissues were observated.Result The results of 5-week experiment had shown:1.In normal mice fed with SF,the blood sugar level was decreased(P<0.05).There were no bovious changes in serum total cholesterol(TC)and the triglyceride(TG).The highdensity-lipoprotein chloesterol(HDL-C) level was increased significantly(P<0.05);2Among DM mice,blood sugar was decrased considerably(P<0.001),TC and TG also decreased noticeably(P<0.05),HDL-C increased clearly(P<0.05);3\\SF has a protective role to the liver and kidney of DM mice.Conclusion:It is suggested that SF has the function of decreasing blood sugan,blood-lipid and improving their metabolism.and of protecting liver and kidney of DM mice.

  10. Effects of Soybean Fibers on Blood Sugar, Lipid Levels and Hepatic_Nephritic Histomorphology in Mice With Diabetes Mellitus

    Institute of Scientific and Technical Information of China (English)

    2001-01-01

    Objective To study the influence of soybean fibers on blood sugar, blood_lipid metabolism, and histomorphology in mice models with diabetes mellitus (DM). Method After normal mice and mice with Streptozocin (STZ)_induced DM were fed with forage containing a certain amount of soybean fiber (SF) for 5 weeks, blood sugar and blood lipid were determined, and morphological changes of hepatic and nephritic tissues were observated. Result The results of 5_week experiment had shown: 1. In normal mice fed with SF, the blood sugar level was decreased (P<0.05). There were no obvious changes in serum total cholesterol (TC) and the triglyceride (TG). The high_density_lipoprotein cholesterol (HDL_C) level was increased significantly (P<0.05); 2. Among DM mice, blood sugar was decreased considerably (P<0.001), TC and TG also decreased noticeably (P<0.05), HDL_C increased clearly (P<0.05); 3. SF has a protective role to the liver and kidney of DM mice. Conclusion It is suggested that SF has the function of decreasing blood sugar, blood_lipid and improving their metabolism, and of protecting liver and kidney of DM mice.

  11. FADS1 FADS2 Gene Cluster, PUFA Intake and Blood Lipids in Children: Results from the GINIplus and LISAplus Studies

    OpenAIRE

    Standl, Marie; Lattka, Eva; Stach, Barbara; Koletzko, Sibylle; Bauer, Carl-Peter; von Berg, Andrea; Berdel, Dietrich; Krämer, Ursula; Schaaf, Beate; Röder, Stefan; Herbarth, Olf; Buyken, Anette; Drogies, Tim; Thiery, Joachim; Koletzko, Berthold

    2012-01-01

    Background Elevated cholesterol levels in children can be a risk factor for cardiovascular diseases in later life. In adults, it has been shown that blood lipid levels are strongly influenced by polymorphisms in the fatty acid desaturase (FADS) gene cluster in addition to nutritional and other exogenous and endogenous determinants. Our aim was to investigate whether lipid levels are determined by the FADS genotype already in children and whether this association interacts with dietary intake ...

  12. Air pollution and blood lipid markers levels: Estimating short and long-term effects on elderly hypertension inpatients complicated with or without type 2 diabetes.

    Science.gov (United States)

    Xiao, Sanhua; Liu, Ranran; Wei, Youxiu; Feng, Lin; Lv, Xuemin; Tang, Fei

    2016-08-01

    With the development of society and the economy, many Chinese cities are shrouded in pollution haze for much of the year. Scientific studies have identified various adverse effects of air pollutants on human beings. However, the relationships between air pollution and blood lipid levels are still unclear. The objective of this study is to explore the short and long-term effects of air pollution on eight blood lipid markers among elderly hypertension inpatients complicated with or without type 2 diabetes (T2D). Blood lipid markers which met the pre-established inclusion criteria were exported from the medical record system. Air pollution data were acquired from the official environmental protection website. Associations between the air quality index and the blood lipid indexes were analyzed by one-way ANOVA and further Bonferroni correction. In an exposure time of 7 days or longer, blood lipid markers were somewhat affected by poor air quality. However, the results could not predict whether atherosclerosis would be promoted or inhibited by poorer air condition. Changes of blood lipid markers of hypertension inpatients with or without T2D were not completely the same, but no blood lipid markers had an opposite trend between the two populations. The air quality index was associated with changes to blood lipid markers to some extent in a population of hypertension inpatients with or without T2D. Further studies are needed to investigate the potential mechanism by which air pollutants induce blood lipids changes. PMID:27180144

  13. Effect of Probiotics on Blood Lipid Concentrations: A Meta-Analysis of Randomized Controlled Trials.

    Science.gov (United States)

    Cho, Young Ae; Kim, Jeongseon

    2015-10-01

    Previous clinical studies have reported mixed results regarding the effect of probiotics on lipid metabolism. Therefore, we conducted a meta-analysis of randomized controlled trials to quantify the direction and magnitude of the potential effect of probiotics on blood lipid concentrations.Eligible studies were randomized, placebo-controlled trials whose interventions were probiotic products containing live bacteria. The studies reported net changes in lipid profiles (total cholesterol, LDL cholesterol, HDL cholesterol, and triglycerides) and their associated standard deviations (or the data to calculate them). The probiotic products did not contain prebiotics or other active ingredients, and the full article was accessible in English.The pooled mean net change in lipid profiles and 95% confidence intervals (95% CIs) were calculated. Q statistics and I were calculated to examine heterogeneity. Potential sources of heterogeneity were investigated via subgroup and sensitivity analyses, and publication biases were estimated.A total of 30 randomized controlled trials with 1624 participants (828 in intervention groups and 796 in placebo groups) were included in this analysis. Subjects treated with probiotics demonstrated reduced total cholesterol and LDL cholesterol compared to control subjects by 7.8 mg/dL (95% CI: -10.4, -5.2) and 7.3 mg/dL (95% CI: -10.1, -4.4), respectively. There was no significant effect of probiotics on HDL cholesterol or triglycerides. The effect of probiotics on total cholesterol and LDL cholesterol depended on a variety of factors. The significant effects were greater for higher baseline total cholesterol levels, longer treatment durations, and certain probiotic strains. In addition, these associations seem stronger in studies supported by probiotics companies.The studies included in this meta-analysis showed significant heterogeneity as indicated by the Q statistics and I. In addition, industry sponsorship may affect study findings

  14. Fitness, body composition and blood lipids following 3 concurrent strength and endurance training modes.

    Science.gov (United States)

    Eklund, Daniela; Häkkinen, Arja; Laukkanen, Jari Antero; Balandzic, Milica; Nyman, Kai; Häkkinen, Keijo

    2016-07-01

    This study investigated changes in physical fitness, body composition, and blood lipid profile following 24 weeks of 3 volume-equated concurrent strength and endurance training protocols. Physically active, healthy male and female participants (aged 18-40 years) performed strength and endurance sessions on different days (DD; men, n = 21; women, n = 18) or in the same session with endurance preceding strength (ES; men, n = 16; women, n = 15) or vice versa (SE; men, n = 18; women, n = 14). The training volume was matched in all groups. Maximal leg press strength (1-repetition maximum (1RM)) and endurance performance (maximal oxygen consumption during cycling), body composition (dual-energy X-ray absorptiometry), and blood lipids were measured. 1RM and maximal oxygen consumption increased in all groups in men (12%-17%, p DD vs. ES and SE both in men (p = 0.003-0.008) and women (p = 0.008-0.009). Total body lean mass increased in all groups (3%-5%, p DD led to decreased total body fat (men, -14% ± 15%, p DD (r = 0.550, p = 0.001). In conclusion, all modes resulted in increased physical fitness and lean mass, while only DD led to decreases in fat mass. Same-session SE and ES combined training is effective in improving physical fitness while volume-equated, but more frequent DD training may be more suitable for optimizing body composition and may be possibly useful in early prevention of cardiovascular and metabolic diseases. PMID:27351384

  15. Interactions of Several Lipid-Related Gene Polymorphisms and Cigarette Smoking on Blood Pressure Levels

    Directory of Open Access Journals (Sweden)

    Rui-Xing Yin, Dong-Feng Wu, Jin-Zhen Wu, Xiao-Li Cao, Lynn Htet Htet Aung, Lin Miao, Xing-Jiang Long, Wan-Ying Liu, Lin Zhang, Meng Li

    2012-01-01

    Full Text Available The interactions of single nucleotide polymorphisms (SNPs and cigarette smoking on blood pressure levels are limited. The present study was undertaken to detect nine lipid-related SNPs and their interactions with cigarette smoking on blood pressure levels. Genotyping of ATP-binding cassette transporter A1 (ABCA-1 V825I, acyl-CoA:cholesterol acyltransferase-1 (ACAT-1 rs1044925, low density lipoprotein receptor (LDL-R AvaⅡ, hepatic lipase gene (LIPC -250G>A, endothelial lipase gene (LIPG 584C>T, methylenetetrahydrofolate reductase (MTHFR 677C>T, proprotein convertase subtilisin-like kexin type 9 (PCSK9 E670G, peroxisome proliferator-activated receptor delta (PPARD +294T>C, and Scavenger receptor class B type 1 (SCARB1 rs5888 was performed in 935 nonsmokers and 845 smokers. The interactions were detected by factorial regression analysis. The frequencies of genotypes (ACAT-1 and LIPG, alleles (ABCA-1, and both genotypes and alleles (LDL-R, LIPC, PPARD and SCARB1 were different between nonsmokers and smokers (P < 0.05-0.001. The levels of pulse pressure (PP, ABCA-1, and systolic, diastolic blood pressure (SBP, DBP and PP (LIPC in nonsmokers were different among the genotypes (P < 0.01-0.001. The levels of SBP (ABCA-1, ACAT-1, LIPG and PCSK9, DBP (ACAT-1, LDL-R, LIPC, PCSK9 and PPARD, and PP (LIPC, LIPG, MTHFR and PCSK9 in smokers were different among the genotypes (P < 0.01-0.001. The SNPs of ABCA-1, ACAT-1 and PCSK9; ACAT-1, LDL-R, MTHFR and PCSK9; and ABCA-1, LIPC, PCSK9 and PPARD were shown interactions with cigarette smoking to influence SBP, DBP and PP levels (P < 0.05-0.001; respectively. The differences in blood pressure levels between the nonsmokers and smokers might partly result from different interactions of several SNPs and cigarette smoking.

  16. Phenotypical and functional characteristics of mesenchymal stem cells derived from equine umbilical cord blood.

    Science.gov (United States)

    Mohanty, N; Gulati, B R; Kumar, R; Gera, S; Kumar, S; Kumar, P; Yadav, P S

    2016-08-01

    Mesenchymal stem cells (MSCs) offer promise as therapeutic aid in the repair of tendon and ligament injuries in race horses. Fetal adnexa is considered as an ideal source of MSCs due to many advantages, including non-invasive nature of isolation procedures and availability of large tissue mass for harvesting the cells. However, MSCs isolated from equine fetal adnexa have not been fully characterized due to lack of species-specific markers. Therefore, this study was carried out to isolate MSCs from equine umbilical cord blood (UCB) and characterize them using cross-reactive markers. The plastic-adherent cells could be isolated from 13 out of 20 (65 %) UCB samples. The UCB derived cells proliferated till passage 20 with average cell doubling time of 46.40 ± 2.86 h. These cells expressed mesenchymal surface markers but did not express haematopoietic/leucocytic markers by RT-PCR and immunocytochemistry. The phenotypic expression of CD29, CD44, CD73 and CD90 was shown by 96.36 ± 1.28, 93.40 ± 0.70, 73.23 ± 1.29 and 46.75 ± 3.95 % cells, respectively in flow cytometry, whereas, reactivity against the haematopoietic antigens CD34 and CD45 was observed only in 2.4 ± 0.20 and 0.1 ± 0.0 % of cells, respectively. Osteogenic and chondrogenic differentiation could be achieved using established methods, whereas the optimum adipogenic differentiation was achieved after supplementing media with 15 % rabbit serum and 20 ng/ml of recombinant human insulin. In this study, we optimized methodology for isolation, cultural characterization, differentiation and immunophenotyping of MSCs from equine UCB. Protocols and markers used in this study can be employed for unequivocal characterization of equine MSCs. PMID:25487085

  17. EVALUATION OF THE EFFECT OF âWAKOUBA '' ON THE LIPID PROFILE, SYSTOLIC BLOOD PRESSURE (SBP) DIASTOLIC (DBP) AND BLOOD GLUCOSE IN HYPERTENSIVE RABBITS

    OpenAIRE

    Tiekpa Wawa J; Bahi C; N’guessan J.D; Koutou A

    2014-01-01

    This study aims at evaluating the effects of Wakouba, an extract of the fronds of oil palm tree Elaeis guineensis (Jacq) traditionally used in the treatment of high blood pressure, on lipid profile, urea, creatinine, blood glucose, systolic blood pressure (SBP), diastolic (DBP) and heart rate (HR) in hypertensive rabbits. Methods: Twenty four rabbits (24) divided into six (06) groups of four rabbits each weighing between 1.5 ±3.0 to 2± 1.5 kg were used. Group 1 served as witness, group 2 sick...

  18. Effects of blood transportation on human peripheral mononuclear cell yield, phenotype and function: implications for immune cell biobanking.

    Directory of Open Access Journals (Sweden)

    Anita Posevitz-Fejfár

    Full Text Available Human biospecimen collection, processing and preservation are rapidly emerging subjects providing essential support to clinical as well as basic researchers. Unlike collection of other biospecimens (e.g. DNA and serum, biobanking of viable immune cells, such as peripheral blood mononuclear cells (PBMC and/or isolated immune cell subsets is still in its infancy. While certain aspects of processing and freezing conditions have been studied in the past years, little is known about the effect of blood transportation on immune cell survival, phenotype and specific functions. However, especially for multicentric and cooperative projects it is vital to precisely know those effects. In this study we investigated the effect of blood shipping and pre-processing delay on immune cell phenotype and function both on cellular and subcellular levels. Peripheral blood was collected from healthy volunteers (n = 9: at a distal location (shipped overnight and in the central laboratory (processed immediately. PBMC were processed in the central laboratory and analyzed post-cryopreservation. We analyzed yield, major immune subset distribution, proliferative capacity of T cells, cytokine pattern and T-cell receptor signal transduction. Results show that overnight transportation of blood samples does not globally compromise T- cell subsets as they largely retain their phenotype and proliferative capacity. However, NK and B cell frequencies, the production of certain PBMC-derived cytokines and IL-6 mediated cytokine signaling pathway are altered due to transportation. Various control experiments have been carried out to compare issues related to shipping versus pre-processing delay on site. Our results suggest the implementation of appropriate controls when using multicenter logistics for blood transportation aiming at subsequent isolation of viable immune cells, e.g. in multicenter clinical trials or studies analyzing immune cells/subsets. One important conclusion might

  19. The Effect of Trans-Chalcone on Amylase Activity, Blood Glucose and Lipid Levels in Diabetic and Non Diabetic Rats

    OpenAIRE

    Najafian, M.; Ebrahim-Habibi, A.; P Yaghmaei; Parivar, K.; Larijani, B.

    2011-01-01

    Introduction & Objective: Alpha amylase is the most important decomposing enzyme in starch. Digestion and absorption of starch in the intestine can be prevented and also the blood sugar levels can be controlled by restrain and control of alpha amylase. The aim of this study was to evaluate the effect of trans-chalcone on amylase activity, blood glucose and lipid levels in diabetic and non diabetic rats. Materials & Methods: This experimental study was conducted in 1388 at Tehran Universit...

  20. Effects of Encapsulated Propolis on Blood Glycemic Control, Lipid Metabolism, and Insulin Resistance in Type 2 Diabetes Mellitus Rats

    OpenAIRE

    Yajing Li; Minli Chen; Hongzhuan Xuan; Fuliang Hu

    2012-01-01

    The present study investigates the encapsulated propolis on blood glycemic control, lipid metabolism, and insulin resistance in type 2 diabetes mellitus (T2DM) rats. The animal characteristics and biological assays of body weight, fasting blood glucose (FBG), fasting serum insulin (FINS), insulin act index (IAI), triglycerides (TG), total cholesterol (TC), high-density lipoprotein cholesterol (HDL-C), and low-density lipoprotein cholesterol (LDL-C) were measured and euglycemic hyperinsulinemi...

  1. Effect of Cucurbita ficifolia and Probiotic Yogurt Consumption on Blood Glucose, Lipid Profile, and Inflammatory Marker in Type 2 Diabetes

    OpenAIRE

    Azade Bayat; Fatemeh Azizi-Soleiman; Motahar Heidari-Beni; Awat Feizi; Bijan Iraj; Reza Ghiasvand; Gholamreza Askari

    2016-01-01

    Background: Control of blood sugar, hypertension, and dyslipidemia are key factors in diabetes management. Cucurbita ficifolia (pumpkin) is a vegetable which has been used traditionally as a remedy for diabetes in Iran. In addition, consumption of probiotics may have beneficial effects on people with Type 2 diabetes. The aim of this study was an investigation of the effects of C. ficifolia and probiotic yogurt consumption alone or at the same time on blood glucose and serum lipids in diabetic...

  2. Effect of n-3 fatty acid supplementation on blood glucose, lipid profile and cytokines in humans: A pilot study

    OpenAIRE

    Raghu, B.; Venkatesan, P

    2008-01-01

    This study was undertaken to evaluate the effects of n-3 fatty acid supplementation on blood glucose, lipid profile and cytokines in humans. Twenty adult healthy subjects were supplemented with 1g/day fish oil concentrate capsules for 2 weeks. Fasting blood samples were taken at baseline and again after 2 week intervention. Fish oil supplementation significantly lowered fasting serum concentrations of total cholesterol, triacylglycerol, very low density lipoprotein and low density lipoprotein...

  3. Blood Pressure Regulation VIII: Resistance Vessel Tone and Implications for a Pro-Atherogenic Conduit Artery Endothelial Cell Phenotype

    OpenAIRE

    Padilla, Jaume; Jenkins, Nathan T.; Laughlin, M. Harold; Fadel, Paul J.

    2013-01-01

    Dysfunction of the endothelium is proposed as the primary initiator of atherosclerotic peripheral artery disease, which occurs mainly in medium to large-sized conduit arteries of the lower extremities (e.g., iliac, femoral, popliteal arteries). In this review article, we propose the novel concept that conduit artery endothelial cell phenotype is determined, in part, by microvascular tone in skeletal muscle resistance arteries through both changes in arterial blood pressure as well as upstream...

  4. Induction of vascular endothelial phenotype and cellular proliferation from human cord blood stem cells cultured in simulated microgravity

    Science.gov (United States)

    Chiu, Brian; Z-M Wan, Jim; Abley, Doris; Akabutu, John

    2005-05-01

    Recent studies have demonstrated that stem cells derived from adult hematopoietic tissues are capable of trans-differentiation into non-hematopoietic cells, and that the culture in microgravity ( μg) may modulate the proliferation and differentiation. We investigated the application of μg to human umbilical cord blood stem cells (CBSC) in the induction of vascular endothelial phenotype expression and cellular proliferation. CD34+ mononuclear cells were isolated from waste human umbilical cord blood samples and cultured in simulated μg for 14 days. The cells were seeded in rotary wall vessels (RWV) with or without microcarrier beads (MCB) and vascular endothelial growth factor was added during culture. Controls consisted of culture in 1 G. The cell cultures in RWV were examined by inverted microscopy. Cell counts, endothelial cell and leukocyte markers performed by flow-cytometry and FACS scan were assayed at days 1, 4, 7 and at the termination of the experiments. Culture in RWV revealed significantly increased cellular proliferation with three-dimensional (3D) tissue-like aggregates. At day 4, CD34+ cells cultured in RWV bioreactor without MCB developed vascular tubular assemblies and exhibited endothelial phenotypic markers. These data suggest that CD34+ human umbilical cord blood progenitors are capable of trans-differentiation into vascular endothelial cell phenotype and assemble into 3D tissue structures. Culture of CBSC in simulated μg may be potentially beneficial in the fields of stem cell biology and somatic cell therapy.

  5. Effect of trans fatty acid intake on abdominal and liver fat deposition and blood lipids

    DEFF Research Database (Denmark)

    Bendsen, Nathalie Tommerup; Chabanova, E.; Thomsen, Henrik S.;

    2011-01-01

    abdominal and liver fat deposition.Objective:We examined the effect of a high intake of TFA as part of an isocaloric diet on whole-body, abdominal and hepatic fat deposition, and blood lipids in postmenopausal women.Methods:In a 16-week double-blind parallel intervention study, 52 healthy overweight...

  6. The Effects of Physical Training on Blood Lipid Profiles in Adolescents with Insulin-Dependent Diabetes Mellitus.

    Science.gov (United States)

    Campaigne, B. N.; And Others

    1985-01-01

    Fourteen adolescents with insulin-dependent diabetes mellitus (IDDM) participated in a 12-week exercise program to determine whether such training would bring about changes in blood lipid and lipoprotein profiles. The findings support the beneficial effects of regular exercise for individuals with IDDM. (MT)

  7. Effect of carotenoid source and dietary lipid content on blood astaxanthin concentration in rainbow trout (Oncorhynchus mykiss)

    OpenAIRE

    Barbosa, M. J.; Morais, R; Choubert, G. (collab.)

    1999-01-01

    Astaxanthin concentration in the blood of rainbow trout was studied in a feeding trial with two different astaxanthin sources: green algae Haematococcus pluvialis and commercial beadlets of 8% astaxanthin content (CAROPHYLL® Pink), and two different dietary lipid levels. The green algae contained 1.4% of carotenoids on a dry matter basis: free astaxanthin (

  8. Study of the Effect of Garlic on Serum Lipids and Blood Glucose Levels in Type 2 Diabetic Patients

    Directory of Open Access Journals (Sweden)

    M Afkhami - Ardekani

    2005-04-01

    Full Text Available Introduction: Hyperlipidemia and diabetes are common risk factors for ischemic heart disease, which is the main cause of mortality in diabetic patients. Strict control of blood glucose and other risk factors in diabetics has led to prevention of complications. Garlic has received particular attention for control of blood glucose and decrease in blood lipid levels. At present, several studies have been carried out in order to prove advantages of garlic. Methods: In this study, effects of garsin (a derivative of garlic present in our country on serum lipids and blood glucose levels in diabetes mellitus type 2 patients was observed. Forty-five type 2 diabetics who had hyperlipidemia were selected. These patients were kept on treatment with 3 tablets of Garsin / day for 4 weeks. Serum lipids and blood glucose levels were measured prior to and at the end of treatment. Results: Relationship between sex and response to treatment in this study was meaningful, such that Gsarsin led to decrease in LDL-C and increase in HDL in females. Conclusion: Therefore, Garsin can be used as an adjunct to treatment in diabetes type 2 patients with hyperlipidemia.

  9. Lipid structure does not modify incorporation of EPA and DHA into blood lipids in healthy adults: a randomised-controlled trial.

    Science.gov (United States)

    West, Annette L; Burdge, Graham C; Calder, Philip C

    2016-09-01

    Dietary supplementation is an effective means to improve EPA and DHA status. However, it is unclear whether lipid structure affects EPA+DHA bioavailability. We determined the effect of consuming different EPA and DHA lipid structures on their concentrations in blood during the postprandial period and during dietary supplementation compared with unmodified fish oil TAG (uTAG). In a postprandial cross-over study, healthy men (n 9) consumed in random order test meals containing 1·1 g EPA+0·37 g DHA as either uTAG, re-esterified TAG, free fatty acids (FFA) or ethyl esters (EE). In a parallel design supplementation study, healthy men and women (n 10/sex per supplement) consumed one supplement type for 12 weeks. Fatty acid composition was determined by GC. EPA incorporation over 6 h into TAG or phosphatidylcholine (PC) did not differ between lipid structures. EPA enrichment in NEFA was lower from EE than from uTAG (P=0·01). Plasma TAG, PC or NEFA DHA incorporation did not differ between lipid structures. Lipid structure did not affect TAG or NEFA EPA incorporation and PC or NEFA DHA incorporation following dietary supplementation. Plasma TAG peak DHA incorporation was greater (P=0·02) and time to peak shorter (P=0·02) from FFA than from uTAG in men. In both studies, the order of EPA and DHA incorporation was PC>TAG>NEFA. In conclusion, EPA and DHA lipid structure may not be an important consideration in dietary interventions. PMID:27424661

  10. Flavonoid-rich beverage effects on lipid profile and blood pressure in diabetic patients

    Institute of Scientific and Technical Information of China (English)

    Reza; Amani; Sara; Moazen; Hajieh; Shahbazian; Kambiz; Ahmadi; Mohammad; Taha; Jalali

    2014-01-01

    AIM: To compare freeze-dried strawberry(FDS) beverage and strawberry-flavored drink effects on lipid profile and blood pressure in type 2 diabetic(T2D) patients.METHODS: In a randomized, double-blind, controlled trial, 36 subjects with T2D(23 females; mean ± SE age: 51.57 ± 10 years) were randomly divided into two groups. Participants consumed two cups of either pure FDS beverage(each cup containing 25 g freeze-dried strawberry powder equivalent to one serving of fresh strawberries; intervention group) or an iso-caloric drinkwith strawberry flavoring(similar to the FDS drink in fiber content and color; placebo group) daily for 6 wk. Anthropometric measurements, 3 d, 24 h dietary recall, and fasting blood samples were collected at baseline and at weeks 6 intervention. After lying down and relaxing for approximately 10 min, each participant’s blood pressure was recorded in triplicate with 5 min intervals; recordings were made at baseline and the trial end-point. Each participant’s lipid profile was assessed before and after intervention.RESULTS: Assessment at the weeks 6 intervention showed a significant reduction from baseline in total cholesterol levels and total cholesterol to high-density lipoprotein cholesterol(HDL-C) ratio in the intervention group(179.01 ± 31.86 to 165.9 ± 32.4 mg/L; P = 0.00 and 3.9 ± 0.88 to 3.6 ± 0.082 mg/L; P = 0.00 respectively), but the change was not significantly different between the two groups(P = 0.07, P = 0.29 respectively). Systolic blood pressure levels were significantly reduced from baseline in both the FDS and placebo drink groups(129.95 ± 14.9 to 114.3 ± 27.5 mm Hg; P = 0.02 and 127.6 ± 15.6 to 122.9 ± 14.47 mm Hg; P = 0.00 respectively), but the reduction was not significantly different between the two groups. Diastolic blood pressure was significantly reduced post-intervention in the FDS drink group compared to placebo group(78.7 ± 7.2 vs 84.4 ± 5.8; P = 0.01), the reduction was also significant within the

  11. Multicomponent blood lipid analysis by means of near infrared spectroscopy, in geese.

    Science.gov (United States)

    Bazar, George; Eles, Viktoria; Kovacs, Zoltan; Romvari, Robert; Szabo, Andras

    2016-08-01

    This study provides accurate near infrared (NIR) spectroscopic models on some laboratory determined clinicochemical parameters (i.e. total lipid (5.57±1.95 g/l), triglyceride (2.59±1.36 mmol/l), total cholesterol (3.81±0.68 mmol/l), high density lipoprotein (HDL) cholesterol (2.45±0.58 mmol/l)) of blood serum samples of fattened geese. To increase the performance of multivariate chemometrics, samples significantly deviating from the regression models implying laboratory error were excluded from the final calibration datasets. Reference data of excluded samples having outlier spectra in principal component analysis were not marked as false. Samples deviating from the regression models but having non outlier spectra in PCA were identified as having false reference constituent values. Based on the NIR selection methods, 5% of the reference measurement data were rated as doubtful. The achieved models reached R(2) of 0.864, 0.966, 0.850, 0.793, and RMSE of 0.639 g/l, 0.232 mmol/l, 0.210 mmol/l, 0.241 mmol/l for total lipid, triglyceride, total cholesterol and HDL cholesterol, respectively, during independent validation. Classical analytical techniques focus on single constituents and often require chemicals, time-consuming measurements, and experienced technicians. NIR technique provides a quick, cost effective, non-hazardous alternative method for analysis of several constituents based on one single spectrum of each sample, and it also offers the possibility for looking at the laboratory reference data critically. Evaluation of reference data to identify and exclude falsely analyzed samples can provide warning feedback to the reference laboratory, especially in the case of analyses where laboratory methods are not perfectly suited to the subjected material and there is an increased chance of laboratory error. PMID:27216674

  12. Effect of dietary fructans and chromium(III supplementation on apparent fat digestibility and blood lipid indices in rat

    Directory of Open Access Journals (Sweden)

    Zbigniew Krejpcio

    2009-06-01

    Full Text Available Background. Fructans and chromium(III are believed to influence lipid and carbohydrate metabolism in animal and human organisms, that can be applied in regulation or improvement of lipid indices, however the importance of their effect is still unclear. This study aimed at evaluation of the effect of inulin-type fructans and chromium(III supplementation on fat digestibility and blood lipid indices in rats. Material and methods. In a three-factorial design 56 male Wistar rats were fed ad libitum semipurified diets (AIN-93 supplemented with two types and levels of fructans (inulin or oligofructose, 5% or 10% of diet and two levels of chromium(III (0.5 or 5 mg/kg of diet; as Cr(III propionate complex for 10 weeks. Apparent fat digestibility and blood lipid profile were determined. Results. It was found that experimental factors did not influence apparent fat digestibility, serum total cholesterol, HDL and HDL cholesterol concentrations, however serum TAG level was significantly lower in animals fed oligofructose vs. inulin diets, high-fructan vs. low-fructan diet. Conclusions. Inulin type fructans and supplementary Cr(III do not affect apparent fat digestibility, fructans however influence lipid metabolism, lowering serum TAG levels in rat.

  13. Metabolic phenotyping of blood plasma by 1H-NMR spectroscopy to detect lung cancer?

    OpenAIRE

    Louis,Evelyne; MESOTTEN, Liesbet; Thomeer, Michiel; Vanhove, Karolien; Vandeurzen, K.; Sadowska, A.; Reekmans, Gunter; Adriaensens, Peter

    2013-01-01

    Introduction. Lung cancer is the leading cause of cancer death worldwide. An effective method which allows to detect lung cancer is urgently needed. Accumulating evidence shows that the metabolism of cancer cells differs from that of normal cells(1). Disturbances in biochemical pathways which occur during the development of cancer provoke changes in the metabolic phenotype(2). Objective. To determine the metabolic phenotype of lung cancer by means of proton nuclear magnetic resonance (1H-...

  14. Effects of Zinc Supplementation on the Anthropometric Measurements, Lipid Profiles and Fasting Blood Glucose in the Healthy Obese Adults

    Directory of Open Access Journals (Sweden)

    Sepide Mahluji

    2013-02-01

    Full Text Available Purpose: The aim of this study was to assess the effects of zinc supplementation on anthropometric measures, improving lipid profile biomarkers, and fasting blood glucose level in obese people. Methods: This randomized, double- blind clinical trial was carried out on 60 obese participants in the 18-45 age range for one month. The participants were randomly divided into the intervention group, who received 30 mg/d zinc gluconate, and the placebo group who received 30mg/d starch. Anthropometric measurements (body mass index (BMI, weight and waist circumference were recorded before and at the end of study. Lipid profile biomarkers and fasting blood glucose were determined using enzymatic procedure. Analysis of Covariance (ANCOVA test was run to compare the post-treatment values of the two groups, and t-test was conducted to compare within group changes. Results: Serum zinc concentration was increased significantly in intervention group (p=0.024. BMI and body weight was significantly decreased (p=0.030 and p=0.020, respectively. Lipid profile biomarkers and fating blood glucose did not change significantly but triglyceride level was significantly decreased (p=0.006 in the intervention group. Conclusion: The obtained results indicate that zinc supplementation improves BMI, body weight, and triglyceride concentration without considerable effects on lipid profile and glucose level. Zinc can be suggested as a suitable supplementation therapy for obese people, but more studies are needed to verify the results.

  15. Prevalence of Phenotypes and Genes of ABO and Rhesus (Rh) Blood Groups in Faisalabad, Pakistan

    OpenAIRE

    Amjad Hameed; Wajahat Hussain; Janbaz Ahmed; Fazli-Rabbi; Javed Anver Qureshi

    2002-01-01

    The frequencies of ABO and Rhesus (D) blood groups and influence of gender were determined on prevalence of these blood groups. Blood group B was found to be the most frequent (38.004%). The frequency of blood group O was 28.755%, for group A 23.260% and for group AB 9.981%. In Rhesus blood grouping system the frequency of Rhesus (D) positive was 89.094%. Gene frequencies with respect to ABO and Rhesus (D) negative were different as for only ABO and ABO along with Rhesus (D) positive. The tre...

  16. Low Levels of Blood Lipids Are Associated with Etiology and Lethal Outcome in Acute Liver Failure

    OpenAIRE

    Manka, Paul; Olliges, Verena; Bechmann, Lars P.; Schlattjan, Martin; Jochum, Christoph; Treckmann, Jürgen W.; Saner, Fuat H; Gerken, Guido; Syn, Wing-Kin; Canbay, Ali

    2014-01-01

    Background/Aims Emerging data links different aspects of lipid metabolism to liver regeneration. In patients with acute liver failure (ALF), low levels of lipids may correlate with disease severity. Thus, we determined whether there is an etiology-specific link between lipid levels in patients suffering from ALF and aimed to investigate an effect of lipid levels on the prognosis of ALF. Methods In this retrospective single center study, we reviewed 89 consecutive ALF patients, who met the cri...

  17. Effects of Flavonoids in Morus indica on Blood Lipids and Glucose in Hyperlipidemia-diabetic Rats

    Institute of Scientific and Technical Information of China (English)

    MA Dong-qing; JIANG Zheng-ju; XU Shi-qiang; YU Xing; HU Xia-min; PAN Hong-yan

    2012-01-01

    Objective To investigate the effect and possible mechanism of flavonoids extracted from Morus indica(FMI)on blood lipids and glucose.Methods The experimental hyperlipidemia-diabetic rats were induced by high-fat diet(HFD)and low dose of Streptozotocin(STZ).Flavonoids-treated rats were pretreated with FMI(50,100,and 200 mg/kg).The plasma,skeletal muscle,and livers were isolated for biochemical assays,HE staining,immunohistochemistry,and Western blotting analysis.Results The results showed that the body weight in flavonoidstreated(100 and 200 mg/kg)rats was reduced(P<0.05,0.01)compared to HFD-fed rats.FMI obviously reduced total cholesterol(P<0.01),triglycerides(P<0.05),and low-density lipoprotein cholesterol(P<0.05),increased high-density lipoprotein(P<0.05),and significantly decreased the atherosclerosis index(P<0.01);FMI(100 and 200 mg/kg)also down-regulated the elevation of blood glucose induced by STZ(P<0.05,0.01);At the meantime,FMI increased hepatic superoxide dismutase(SOD)activity and reduced hepatic malondialdehyde(MDA)content obviously.In addition,the results showed that the expression of hepatic CYP2E1 was markedly decreased while the expression of GLUT-4 in skeletal muscles was increased by FMI.Conclusion The above results demonstrate that the effect of FMI is related to its up-regulation of hepatic SOD activity,reduction of hepatic MDA content,down-regulation of hepatic CYP2E1 expression,and increase of GLUT-4 expression in skeletal muscle,which suggests that FMI may prevent or improve hyperlipidemia and hyperglycemia caused by an excessive HFD.

  18. Effects of Gongronema latifolium on blood lipids, lipoproteins and glucose values in adult Nigerians

    Directory of Open Access Journals (Sweden)

    Rosemary Adamma Analike

    2015-04-01

    Full Text Available Background: The study evaluates the beneficial effects or otherwise of Gongronema latifolium (utazi consumption on plasma lipid profile and blood glucose in healthy subjects. Methods: The study was conducted on twenty (20 apparently healthy subjects (10 males and 10 females, within the age range of 20-55years who were randomly recruited from Nnamdi Azikiwe University Teaching Hospital (NAUTH staff and medical students in Nnewi Campus. Twenty experimental subjects (10 males and 10 females were fed with 5g/day of fresh Gongronema latifolium leaves for six weeks. Blood samples were collected at baseline and every week for six weeks and the biochemical parameters analyzed using standard laboratory methods. Results: There were significant reductions in the levels of plasma glucose (3.85 +/- 0.14 vs. 4.92 +/- 0.31 mmol/l, cholesterol (3.60 +/- 0.43 vs. 4.56 +/- 0.67 mmol/l, triglycerides (0.73 +/- 0.19 vs. 0.96 +/- 0.20 mmol/l, Low Density Lipoprotein Cholesterol (LDL-C (1.97 +/- 0.48 vs. 2.70 +/- 0.67 mmol/l and LDL-C/High Density Lipoprotein Cholesterol (HDL-C ratio (1.32 +/- 0.44 vs. 2.11 +/- 0.72 mmol/l of the subjects that were fed with Gongronema latifolium leaves for six weeks compared with their baseline values; all P <0.05. Conclusion: The result of this study showed that Gongronema latifolium has hypoglycemic and hypolipidemic effect on healthy subjects and might be beneficial for the management of diabetes mellitus and cardiovascular diseases. [Int J Res Med Sci 2015; 3(4.000: 891-895

  19. The role of blood cell membrane lipids on the mode of action of HIV-1 fusion inhibitor sifuvirtide

    International Nuclear Information System (INIS)

    Research highlights: → Sifuvirtide interacts with erythrocyte and lymphocyte membrane in a concentration dependent manner by decreasing its dipole potential. → Dipole potential variations in lipid vesicles show sifuvirtide's lipid selectivity towards saturated phosphatidylcholines. → This peptide-membrane interaction may direct the drug towards raft-like membrane domains where the receptors used by HIV are located, facilitating its inhibitory action. -- Abstract: Sifuvirtide is a gp41 based peptide that inhibits HIV-1 fusion with the host cells and is currently under clinical trials. Previous studies showed that sifuvirtide partitions preferably to saturated phosphatidylcholine lipid membranes, instead of fluid-phase lipid vesicles. We extended the study to the interaction of the peptide with circulating blood cells, by using the dipole potential sensitive probe di-8-ANEPPS. Sifuvirtide decreased the dipole potential of erythrocyte and lymphocyte membranes in a concentration dependent manner, demonstrating its interaction. Also, the lipid selectivity of the peptide towards more rigid phosphatidylcholines was confirmed based on the dipole potential variations. Overall, the interaction of the peptide with the cell membranes is a contribution of different lipid preferences that presumably directs the peptide towards raft-like domains where the receptors are located, facilitating the reach of the peptide to its molecular target, the gp41 in its pre-fusion conformation.

  20. Disturbances in lipid second messengers generation by stimulated blood lymphocytes in breast cancer

    Directory of Open Access Journals (Sweden)

    Galstyan H. M.

    2011-10-01

    Full Text Available Aim. The main objective of this study was the comparative investigation of diverse lipid second messenger (LSM generation by human peripheral blood lymphocytes (HPBL at different (5, 10, 30 and 60 s time points of cell co-stimulation by anti-CD3 and anti-CD28 monoclonal antibodies in norm and breast cancer (BC. Methods. Ficoll-Hypaque gradient centrifugation. Results. The data obtained indicate that some mechanisms of LSM generation/utilization in stimulated crude HPBL were significantly altered in BC compared to norm. Particularly, the reliable generation of arachidonyl-1,2-diacylglycerol (1,2-DAG at the initial step (5 s of cell stimulation observed in norm was depressed in BC and reached the value below the basal level in unstimulated cells. It is important that the disturbances in 1,2-DAG formation in HPBL obtained from patients with BC were identical with those observed earlier in other forms of cancer. Conclusions. We conclude that the regularities revealed are common characteristics for all the types of malignancy studied and can be used as additional testing parameters for cancer definition and individual correction of the chemotherapy programs for disease treatment

  1. Disruption of blood-testis barrier dynamics in ether-lipid-deficient mice.

    Science.gov (United States)

    Komljenovic, Dorde; Sandhoff, Roger; Teigler, Andre; Heid, Hans; Just, Wilhelm W; Gorgas, Karin

    2009-08-01

    One of the major roles of Sertoli cells is to establish the blood-testis (Sertoli cell) barrier (BTB), which is permanently assembled and disassembled to accommodate the translocation of leptotene spermatocytes from the basal into the adluminal compartment of the seminiferous epithelium and to guarantee completion of meiosis and spermiogenesis. Recently, we have demonstrated spermatogenesis to be arrested before spermatid elongation in Gnpat-null mice with selective deficiency of ether lipids (ELs) whose functions are poorly understood. In this study, we have focused on the spatio-temporal expression of several BTB tight-junctional proteins in the first wave of spermatogenesis to obtain insights into the physiological role of ELs during BTB establishment and dynamics. Our data confirm the transient existence of Russell's intermediate or translocation compartment delineated by two separate claudin-3-positive luminal and basal tight junctions and reveal that EL deficiency blocks BTB remodeling. This block is associated with (1) downregulation and mistargeting of claudin-3 and (2) impaired BTB disassembly resulting in deficient sealing of the intermediate compartment as shown by increased BTB permeability to biotin. These results suggest that ELs are essential for cyclic BTB dynamics ensuring the sluice mechanism for leptotene translocation into the adluminal compartment. PMID:19495798

  2. Influence of radioiodine therapy on the immuno phenotype of peripheral blood lymphocytes of patients with thyroid cancer of different age

    International Nuclear Information System (INIS)

    The authors present the results of a study of the phenotypic composition of blood lymphocytes in patients with differentiated thyroid cancer of different all age before and on day 6 after radioiodine therapy. The phenotypic profile of peripheral blood cells was determined in patients of three age groups: patients aged under 18 years (group 1), patients whose age was within the range 19 to 40 years (group 2), and patients older than 41 years (group 3). The studies have shown that in patients with thyroid cancer, abnormalities developed in the composition of peripheral blood lymphocytes, which were more pronounced in elderly patients. Under the influence of radioiodine, disturbances in the immune system of patients are aggravated. The most significant after-effects of radioiodine therapy that had been identified in patients of all age groups, were represented by a significant decrease in the number of B-lymphocytes, an increase in natural killer cells and CD95+ lymphocytes. Furthermore, it was found that radioiodine has the most significant effect on the immune system of children and adolescents. In this age group, the content of lymphocyte subpopulations is changing: CD4+, CD8+, IRI, CD19+, CD16+CD56+, CD95+ and HLA-DR, which suggests the need for further dynamic studies in this age group of patients, which is the most sensitive to radioiodine effect in order to determine the extent of immunological disorders and the terms of their recovery

  3. Influence of SNPs in nutrient-sensitive candidate genes and gene-diet interactions on blood lipids

    DEFF Research Database (Denmark)

    Brahe, Lena Kirchner; Angquist, Lars; Larsen, Lesli Hingstrup;

    2013-01-01

    Blood lipid response to a given dietary intervention could be determined by the effect of diet, gene variants or gene-diet interactions. The objective of the present study was to investigate whether variants in presumed nutrient-sensitive genes involved in lipid metabolism modified lipid profile......-cholesterol, HDL-cholesterol and TAG after an 8-week low-energy diet (only main effect), and a 6-month ad libitum weight maintenance diet, with different contents of dietary protein or glycaemic index. After adjusting for multiple testing, a SNP-dietary protein interaction effect on TAG was identified for lipin 1...... pathways, and identified one significant interaction between LPIN1 rs4315495 and dietary protein for TAG concentration....

  4. EVALUATION OF THE EFFECT OF “WAKOUBA '' ON THE LIPID PROFILE, SYSTOLIC BLOOD PRESSURE (SBP DIASTOLIC (DBP AND BLOOD GLUCOSE IN HYPERTENSIVE RABBITS

    Directory of Open Access Journals (Sweden)

    Tiekpa Wawa J

    2014-11-01

    Full Text Available This study aims at evaluating the effects of Wakouba, an extract of the fronds of oil palm tree Elaeis guineensis (Jacq traditionally used in the treatment of high blood pressure, on lipid profile, urea, creatinine, blood glucose, systolic blood pressure (SBP, diastolic (DBP and heart rate (HR in hypertensive rabbits. Methods: Twenty four rabbits (24 divided into six (06 groups of four rabbits each weighing between 1.5 ±3.0 to 2± 1.5 kg were used. Group 1 served as witness, group 2 sick and untreated control, groups 3, 4, 5, and 6 were used as experimental groups (sick+treatment. Throughout the experiment the witness group received distilled water; adrenaline was administered to the sick control group. After 10 days of adrenaline injection, hypertension has been stabilized in sick groups (group 2 to 6 and blood was taken for the determination of urea, creatinine, and lipids. Four (04 of the five (05 groups of hypertensive rabbits were treated with two (2 doses of " Wakouba " 950 and 2500 mg / kg bw and two ( 02 doses of ténordate 10 and 20 mg / kg BW, two(2 group by two(2 group . After ten (10 days of treatment, the SBP and DBP and HR were measured, blood was collected for determination of the same biochemical parameters. Results: The study of the effect of Wakouba and ternodate on the changes in systolic blood pressure SBP, DBP and heart rate (HR showed a significant decrease (P up to normalization of these parameters after 10 days of treatment. Similarly, the measurement of serum lipid profile in hypertensive rabbits treated with Wakouba (950mg / kg bw and tenordate (20 mg / kg bw showed a significant reduction in (P values of total cholesterol, LDL cholesterol and triglycerides in contrast to HDL cholesterol which has increased significantly compared to the control group. Same doses also normalize serum glucose, urea and creatinine. Histological sections performed on the kidney and the heart of hypertensive rabbit showed congestion of

  5. Strongly increasing blood concentrations of lipid-soluble organochlorines in high arctic common eiders during incubation fast.

    Science.gov (United States)

    Bustnes, Jan Ove; Moe, Børge; Herzke, Dorte; Hanssen, Sveinn Are; Nordstad, Tore; Sagerup, Kjetil; Gabrielsen, Geir W; Borgå, Katrine

    2010-04-01

    Female common eiders (Somateria mollissima) starve during the nesting stage and may loose 30-45% of their initial body mass, mostly through lipid mobilization. In this study, the effects of fasting on the blood concentrations of three lipid-soluble organochlorines (OCs: polychlorinated biphenyl [PCB]-153; 1-dichloro-2,2-bis (p-chlorophenyl) ethylene [p,p'-DDE]; and hexachlorobenzene [HCB]) were examined in eiders breeding in the high Arctic. Blood samples were taken from females (n=47) at day 5 and day 20 of the incubation period. The mean wet weight concentrations of PCB-153 and p,p'-DDE increased strongly between day 5 and day 20 (3.6 and 8.2-fold, respectively), while HCB increased less (1.7-fold). There was a strong negative association between daily increase in PCB-153 and clutch size, and a weaker relationship for p,p'-DDE, suggesting that maternal transfer to the eggs is a significant pathway of elimination of OCs in eiders. Moreover, poor body condition (body mass controlled for body size) late in the incubation period was associated with strong daily increase of both p,p'-DDE and PCB-153, which may suggest that the release of these compounds increases when lipid reserves become depleted. For HCB, the increase was mainly associated with increase in blood lipid concentrations, and weakly to the amount of burned lipids. The causes for the differences between the compounds are, however, poorly understood. Although the absolute levels of OCs in eiders were relatively low, their rapid build up during incubation is worrying as it coincides with poor body condition and weakened immune systems. PMID:20156633

  6. Effects of Cinnamomum zeylanicum (Ceylon cinnamon on blood glucose and lipids in a diabetic and healthy rat model

    Directory of Open Access Journals (Sweden)

    Priyanga Ranasinghe

    2012-01-01

    Full Text Available Objectives: To evaluate short- and long-term effects of Cinnamomum zeylanicum on food consumption, body weight, glycemic control, and lipids in healthy and diabetes-induced rats. Materials and Methods: The study was conducted in two phases (Phase I and Phase II, using Sprague-Dawley rats in four groups. Phase I evaluated acute effects on fasting blood glucose (FBG (Groups 1 and 2 and on post-oral glucose (Groups 3 and 4 blood glucose. Groups 1 and 3 received distilled-water and Groups 2 and 4 received cinnamon-extracts. Phase II evaluated effects on food consumption, body weight, blood glucose, and lipids over 1 month. Group A (n = 8, distilled-water and Group B (n = 8, cinnamon-extracts were healthy rats, while Group C (n = 5, distilled-water and Group D (n = 5, cinnamon-extracts were diabetes-induced rats. Serum lipid profile and HbA1c were measured on D-0 and D-30. FBG, 2-h post-prandial blood glucose, body weight, and food consumption were measured on every fifth day. Results: Phase I: There was no significant difference in serial blood glucose values in cinnamon-treated group from time 0 (P > 0.05. Following oral glucose, the cinnamon group demonstrated a faster decline in blood glucose compared to controls (P < 0.05. Phase II: Between D0 and D30, the difference in food consumption was shown only in diabetes-induced rats (P < 0.001. Similarly, the significant difference following cinnamon-extracts in FBG and 2-h post-prandial blood glucose from D0 to D30 was shown only in diabetes-induced rats. In cinnamon-extracts administered groups, total and LDL cholesterol levels were lower on D30 in both healthy and diabetes-induced animals (P < 0.001. Conclusions: C. zeylanicum lowered blood glucose, reduced food intake, and improved lipid parameters in diabetes-induced rats.

  7. Effects of Cinnamomum zeylanicum (Ceylon cinnamon) on blood glucose and lipids in a diabetic and healthy rat model

    OpenAIRE

    Priyanga Ranasinghe; Sanja Perera; Mangala Gunatilake; Eranga Abeywardene; Nuwan Gunapala; Sirimal Premakumara; Kamal Perera; Dilani Lokuhetty; Prasad Katulanda

    2012-01-01

    Objectives: To evaluate short- and long-term effects of Cinnamomum zeylanicum on food consumption, body weight, glycemic control, and lipids in healthy and diabetes-induced rats. Materials and Methods: The study was conducted in two phases (Phase I and Phase II), using Sprague-Dawley rats in four groups. Phase I evaluated acute effects on fasting blood glucose (FBG) (Groups 1 and 2) and on post-oral glucose (Groups 3 and 4) blood glucose. Groups 1 and 3 received distilled-water and Groups 2 a...

  8. Analysis of Time Dependent Low Level Exposure to Gallium Arsenide on Blood ALAD activity, Glutathione and Lipid per oxidation levels in Rat Blood, Liver and Kidney

    Directory of Open Access Journals (Sweden)

    Braham Deo Gupta

    2015-06-01

    Full Text Available Gallium arsenide (GaAs, an intermetallic semiconductor has widespread applications in the electronic industry. GaAs has the ability to dissociate into its constitutive moieties, arsenic and gallium which might be responsible for the oxidative stress. The present study was aimed at evaluating, effect of gallium arsenide on blood ALAD activity, glutathione and lipid per oxidation levels in rat blood, liver and kidney on exposure of 1, 2 and 6 months. Result indicated that arsenic moiety in GaAs was mainly responsible for causing oxidative stress via increased TBARS levels, decreased glutathione levels in blood and tissues. We also noted the decreased activity of ALAD in rat blood on exposure to gallium arsenide. The study demonstrates that the time dependent exposure to low level gallium arsenide led to increased in lipid per oxidation, decrease glutathione level and ALAD activity which concludes that the slow release of arsenic moiety from GaAs is mainly responsible for oxidative stress in rats and exerts its toxicity in time dependent manner related to its dissolution and maximum toxicity by increasing the time period of exposure.

  9. Role of sudarshan kriya and pranayam on lipid profile and blood cell parameters during exam stress: A randomized controlled trial

    OpenAIRE

    Swapna Subramanian; Tamilselvi Elango; Hemamalini Malligarjunan; Vinod Kochupillai; Haripriya Dayalan

    2012-01-01

    Background: Yoga is a science practiced in India over thousands of years. It produces constituent physiological changes and has sound scientific basis. Aim: Since exam stress modifies lipid profile and hematological parameters, we conducted an investigation on the effect of sudarshan kriya (SK and P) program on these parameters. Materials and Methods: Blood samples of 43 engineering students were collected at four intervals namely baseline (BL), exam stress (ES), three and six weeks p...

  10. Effects of Zinc Supplementation on the Anthropometric Measurements, Lipid Profiles and Fasting Blood Glucose in the Healthy Obese Adults

    OpenAIRE

    Sepide Mahluji; Mohammad Asghari Jafarabadi; Nazila Farrin; Yaser Khaje Bishak; Majid Mobasseri; Alireza Ostadrahimi; Laleh Payahoo

    2013-01-01

    Purpose: The aim of this study was to assess the effects of zinc supplementation on anthropometric measures, improving lipid profile biomarkers, and fasting blood glucose level in obese people. Methods: This randomized, double- blind clinical trial was carried out on 60 obese participants in the 18-45 age range for one month. The participants were randomly divided into the intervention group, who received 30 mg/d zinc gluconate, and the placebo group who received 30mg/d starch. Anthropo...

  11. Examining of the effects of aerobic dance and step dance exercises on some hematological parameters and blood lipids

    OpenAIRE

    Halil İbrahim Ceylan; Gönül Babayiğit İrez; Özcan Saygın

    2014-01-01

    The aim of the study was to examine the the effects of aerobic and step dance exercises on hematological and blood lipids parameters. 32 university students were voluntarily participated in this study. They were randomly assigned to aerobic dance (N=16) and step dances (N=16). All groups were participated an hour aerobic or step dance exercise session 2 times in a week during 3 months. Wilcoxon Sign test was used to analyze pretest and posttest results of variables. As results, significant di...

  12. Related research of blood glucose, blood lipid and serum cystatin C and lower-extremity arterial disease in type 2 diabetic mellitus

    Institute of Scientific and Technical Information of China (English)

    Juan Zeng; Jie Ou; Yun-Mei He; Chun-Yu Cai

    2016-01-01

    Objective:To explore the relationship between levels of blood glucose, blood lipid and serum cystatin C and lower-extremity arterial disease in type 2 diabetic mellitus so as to provide the basis for the prevention and treatment of the disease.Methods:A total of 240 cases of patients with type 2 diabetic mellitus receiving physical examinations in our hospital from March 2014 to March 2015 were selected and divided into three groups. The control group was consisted of type 2 diabetes patients without lower-extremity arterial disease, the observation group 1 included type 2 diabetes patients with mild or moderate lower-extremity arterial disease, and the observation group 2 was formed by type 2 diabetes patients with severe lower-extremity arterial disease. Each group contained 80 cases. Then, the heights, weights, waist and hip circumferences of patients in the three groups were determined and the body mass index (BMI) and waist to hip ratio (WHR) were calculated; the fasting blood glucose (FBG), fasting insulin (FINS), insulin resistance index (HOMA-IR), 2 h postprandial blood glucose (2 h PG) and glycosylated hemoglobin (HBA1c) in two groups were detected; and the levels of serum total cholesterol(TC), triglyceride (TG), low density lipoprotein cholesterol (LDL-C), high density lipoprotein cholesterol (HDL-C), apolipoprotein A1 (apoA1), apolipoprotein B (apoB) and cystatin C (CysC) in two groups were also tested.Results:Compared with the control group, the WHR, FBG, HOMA-IR, 2 h PG, HBA1c, LDL-C, apoB and CysC all increased significantly, and the differences were statistically significant. Besides, there were statistically significances existing between the observation groups 1 and 2. What’s more, the serum levels of TC, HDL-C, TG and apoA1 in the observation groups were not significantly different from those of the control group.Conclusions: There is a close relationship between blood glucose, blood lipid, serum cystatin C level and lower-extremity arterial

  13. Blood lipid levels, statin therapy and the risk of intracerebral hemorrhage

    OpenAIRE

    Ma, Yingxu; Li, Zhaokai; Chen, Liang; Li, Xiangping

    2016-01-01

    Dyslipidemia has been proven to play an important role in the occurrence and development of the ischemic stroke and lipid-lowering therapy could significantly decrease the risk of the ischemic stroke. However, the association between lipid levels, lipid-lowering therapy and the risk of intracerebral hemorrhage (ICH) is not clear. Studies have shown that low serum levels of total cholesterol might be associated with increasing risk of ICH, whereas the SPARCL study, a large prospective, randomi...

  14. Vegan diet and blood lipid profiles: a cross-sectional study of pre and postmenopausal women

    OpenAIRE

    Huang, Yee-Wen; Jian, Zhi-Hong; Chang, Hui-Chin; Nfor, Oswald Ndi; Ko, Pei-Chieh; Lung, Chia-Chi; Lin, Long-Yau; Ho, Chien-Chang; Chiang, Yi-Chen; Liaw, Yung-Po

    2014-01-01

    Background Vegan diet has been associated with lower risk of cardiovascular diseases and mortality, partly due to its effects on serum lipid profiles. Lipid profiles [high density lipoprotein-cholesterol (HDL-C), low density lipoprotein-cholesterol (LDL-C) and triglycerides (TG)] have not been fully elucidated either in pre and postmenopausal vegans or in ovo-lacto vegetarians. This study aimed to compare lipid profiles among vegans, ovo-lacto vegetarians and omnivores. Methods Demographic da...

  15. Rapid and simple extraction of lipids from blood plasma and urine for liquid chromatography-tandem mass spectrometry.

    Science.gov (United States)

    Bang, Dae Young; Byeon, Seul Kee; Moon, Myeong Hee

    2014-02-28

    A simple and fast lipid extraction method from human blood plasma and urine is introduced in this study. The effective lipid extraction from biological systems with a minimization of the matrix effect is important for the successful qualitative and quantitative analysis of lipids in liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS). The method described here is based on the modification of the quick, easy, cheap, effective, rugged and safe (QuEChERS) extraction method, which was originally developed for pesticide residue analysis in food, for the purpose of isolating lipids from biological fluids. Applicability of QuEChERS method for lipids was evaluated by varying organic solvents for the extraction/partitioning of lipids in MgSO4/CH3COONa for the removal of water and by varying sorbents (primary secondary amines, graphitized carbon black, silica, strong anion exchange resins and C18 particles) for the dispersive solid-phase extraction (dSPE) step. This study shows that 2:1 (v/v) CHCl3/CH3OH is effective in the extraction/partitioning step and that 50mg of C18 particles (for 0.1mL plasma and 1mL of urine) are more suitable for sample cleanup for the dSPE step of the QuEChERS method. Matrix effects were calculated by comparing the recovery values of lipid standards spiked to both plasma and urine samples after extraction with those of the same standards in a neat solution using nanoflow LC-ESI-MS/MS, resulting in improved MS signals due to the decrease of the ion suppression compared to the conventional Folch method. The modified QuEChERS method was applied to lipid extracts from both human urine and plasma samples, demonstrating that it can be powerfully utilized for high-speed (<15min) preparation of lipids compared to the Folch method, with equivalent or slightly improved results in lipid identification using nLC-ESI-MS/MS. PMID:24491523

  16. The Effects of Probiotic Supplementation on Markers of Blood Lipids, and Blood Pressure in Patients with Prediabetes: A Randomized Clinical Trial

    Science.gov (United States)

    Mahboobi, Sepideh; Iraj, Bijan; Maghsoudi, Zahra; Feizi, Awat; Ghiasvand, Reza; Askari, Gholamreza; Maayeshi, Najmeh

    2014-01-01

    Background: Prediabetes is a high-risk condition for type 2 diabetes mellitus. The growing prevalence of diabetes emphasizes on the necessity of concentrating on various strategies to prediabetes prevention and management. Probiotics as a group of functional foods might exert antidiabetic effects. This study aimed to assess the effects of probiotic administration on blood lipid profile and blood pressure in patients with prediabetes. Methods: This randomized controlled trial consisted of 60 prediabetic patients, aged 25-65 years old, that were randomly assigned to the intervention (receiving 500 mg probiotic capsules, n = 30) or control group (receiving placebo, n = 30) for 8-week period. Demographic and anthropometric data were collected at baseline. Blood samples were collected at baseline and after 8 weeks for biochemical measurements. Blood pressure was measured at the baseline an after 8 weeks of intervention. Data regarding dietary intakes and physical activity were also collected during the study. We used SPSS software version 16 (SPSS Inc. Chicago, USA) for data analyzing. Results: Probiotic supplementation did not contribute to significant changes in total cholesterol, low-density lipoprotein (LDL)-cholesterol, high-density lipoprotein (HDL)-cholesterol, Triglycerides (TG), TG/LDL and LDL/HDL ratios, after 8 weeks. After adjusting for potential confounders, HDL-cholesterol reduced significantly in the placebo group compared with probiotic group. Percent change in systolic blood pressure was significantly different in the probiotic group in comparison with a placebo group (–3.10 ± 2.22 vs. 3.24 ± 1.96, P = 0.01), although this significance did not exist anymore after adjusting for confounders (P > 0.05). Conclusions: Our study showed that probiotics did not have significant effects on lipid markers although they had positive effects on systolic blood pressure. PMID:25400881

  17. Glucose tolerance, blood lipid, insulin and glucagon concentration after single or continuous administration of aspartame in diabetics.

    Science.gov (United States)

    Okuno, G; Kawakami, F; Tako, H; Kashihara, T; Shibamoto, S; Yamazaki, T; Yamamoto, K; Saeki, M

    1986-04-01

    A nutritive sweetener, aspartame (L-aspartyl-L-phenylalanine methylester) was administered orally to normal controls and diabetic patients in order to evaluate effects on blood glucose, lipids and pancreatic hormone secretion. An oral glucose tolerance test was also performed in the same subjects as a control study of aspartame administration. In 7 normal controls and 22 untreated diabetics, a single dose of 500 mg aspartame, equivalent to 100 g glucose in sweetness, induced no increase in blood glucose concentration. Rather, a small but significant decrease in blood glucose was noticed 2 or 3 h after administration. The decrease in blood glucose was found to be smallest in the control and became greater as the diabetes increased in severity. No significant change in blood insulin or glucagon concentration during a 3-h period was observed in either the controls or the diabetics. The second study was designed to determine the effects of 2 weeks' continuous administration of 125 mg aspartame, equal in sweetness to the mean daily consumption of sugar (20-30 g) in Japan, to 9 hospitalized diabetics with steady-state glycemic control. The glucose tolerance showed no significant change after 2 weeks' administration. Fasting, 1 h and 2 h postprandial blood glucose, blood cholesterol, triglyceride and HDL-cholesterol were also unaffected. From these and other published results, aspartame would seem to be a useful alternative nutrient sweetener for patients with diabetes mellitus. PMID:3522147

  18. Effects of Perilla Seed on Blood Lipid Regulation and Lipid Peroxidation of Coturnix coturnix with Hyperlipidemia%紫苏籽对高血脂鹌鹑血脂调节及脂质过氧化的影响

    Institute of Scientific and Technical Information of China (English)

    谭健民; 陈眷华

    2008-01-01

    [Objective] Study on the effect of perilla seed on blood lipid regulation and psroxidation of Coturnix with hyperlipidemia.[Method] The high-lipid diet was fed to establish the high lipid model of Coturnix coturnix. The normal control group, high lipid model group, high dose of perilla seed group(2.5 g/kg), low dose of periUa seed group(0.83 g/kg) were designed to measure the contents of total cholesterol (TC), three acids glyceride (TC), high density lipoprotein cholesterol (HDL-C), low density lipoprctein cholesterol (LDL-C), very low density lipoprotein cholesterol (VLDL-C) and malondialdehyde (MDA), superoxide dismutnse (SOD) activity. [Result] All the test groups could decrease TC,TG,LDL-C and MDA content and significantly increase the HDL-C content (p <0.01) and SOD activity(p <0.01) in serum. [Conclusion] Pcrilla seed has the blood lipid regulation and peroxidation function.

  19. The Autistic Phenotype Exhibits a Remarkably Localized Modification of Brain Protein by Products of Free Radical-Induced Lipid Oxidation

    Directory of Open Access Journals (Sweden)

    Teresa A. Evans

    2008-01-01

    Full Text Available Oxidative damage has been documented in the peripheral tissues of autism patients. In this study, we sought evidence of oxidative injury in autistic brain. Carboxyethyl pyrrole (CEP and iso[4]levuglandin (iso[4]LGE2-protein adducts, that are uniquely generated through peroxidation of docosahexaenoate and arachidonate-containing lipids respectively, and heme oxygenase-1 were detected immunocytochemically in cortical brain tissues and by ELISA in blood plasma. Significant immunoreactivity toward all three of these markers of oxidative damage in the white matter and often extending well into the grey matter of axons was found in every case of autism examined. This striking threadlike pattern appears to be a hallmark of the autistic brain as it was not seen in any control brain, young or aged, used as controls for the oxidative assays. Western blot and immunoprecipitation analysis confirmed neurofilament heavy chain to be a major target of CEP-modification. In contrast, in plasma from 27 autism spectrum disorder patients and 11 age-matched healthy controls we found similar levels of plasma CEP (124.5 ± 57.9 versus 110.4 ± 30.3 pmol/mL, iso[4]LGE2 protein adducts (16.7 ± 5.8 versus 13.4 ± 3.4 nmol/mL, anti-CEP (1.2 ± 0.7 versus 1.2 ± 0.3 and anti-iso[4]LGE2 autoantibody titre (1.3 ± 1.6 versus 1.0 ± 0.9, and no differences between the ratio of NO2Tyr/Tyr (7.81 E-06 ± 3.29 E-06 versus 7.87 E-06 ± 1.62 E-06. These findings provide the first direct evidence of increased oxidative stress in the autistic brain. It seems likely that oxidative injury of proteins in the brain would be associated with neurological abnormalities and provide a cellular basis at the root of autism spectrum disorders.

  20. The effects of hydroalcoholic extract of dracocephalum kotschyi on blood glucose and lipid profile in diabetic rats

    Directory of Open Access Journals (Sweden)

    Majid Eskandari

    2016-03-01

    Full Text Available Background & Objective: Diabetes mellitus has different side effects on various tissues of the body. This study was conducted to determine the effect of hydroalcoholic extract of Dracocephalum kotschyi on blood glucose and lipid profile of streptozotocin-induced diabetic rats. Materials & Methods: In this experimental study, 48 male Wistar rats (200-250 g randomly divided into 6 equal groups. Normal control and control diabetic received distilled water, whereas the control group received the hydroalcoholic extract of Dracocephalum kotschyi (120 mg/Kg bw. Three treatment diabetic groups received 40, 80 and 120 mg/Kg bw of hydroalcoholic extract of Dracocephalum kotschyi, respectively. All rats were treated orally by gavage which continued daily for 21 days. The level of blood glucose and lipid profile was measured at the end of the study in different groups. Results: The Mean blood glucose in the extract treatment groups decreased significantly compared to the diabetic control. The mean of total cholesterol in the treatment groups with 80 and 120 mg/Kg bw of the extract were 105.8±9.73 and 102.73±8.75 mg/dl respectively, that reduced significantly compared to the diabetic control (135.2±12.15 mg/dl. The mean of triglyceride in the treatment groups with 80 and 120 mg/Kg bw of the extract were 177.81±13.47 and 164.86±12.53 mg/dl that reduced significantly compared to the diabetic control (206.75±17.42 mg/dl. Conclusion: The extract of Dracocephalum kotschyi probably has reduced the blood glucose and lipid profile in diabetic mellitus.

  1. Ligation of MHC class I molecules on peripheral blood T lymphocytes induces new phenotypes and functions

    DEFF Research Database (Denmark)

    Bregenholt, S; Röpke, M; Skov, S; Claesson, Mogens Helweg

    1996-01-01

    Microgram concentrations of immobilized anti-MHC class I (MHC-I) Ab induced proliferation of resting CD3+ T cells from peripheral blood. In contrast, soluble Ab did not activate T cells. Exposure of T cells to immobilized anti-MHC-I Ab for only 24 h was followed by proliferation and development of...

  2. The influence of a number of decorporates on the state of lipid peroxidation system of blood of experimental animals

    Energy Technology Data Exchange (ETDEWEB)

    Mazhul, L. M.; Volykhina, V. E.; Gatsko, G. G.; Bagel, J. M. [Institute of Radiobiology of NASB, Minsk (Belarus)

    2002-07-01

    The investigations directed to the search for the substants of radioprophylactic and radiotherapeutical action have important theoretical and practical importance. Since the base of damaging effect of ionizing radiations is the chain free radical reactions initiating the processes of lipid peroxidation in the organism, we carried out the study of the influence of a number of decorporates on the content of Strontium-85 and Caesium-137 in the organism and on the lipid peroxidation system in blood of experimental animals too. The experiments were carried out on the 4-month old male rats to which Strontium-85 (2282 Bq) and Caesium-137 (3101 Bq) - group 4 were administered per os during 30 days. Against a backgourd of daily incorporation of radionuclides, the animals were treated with a number of decorporates and food products: group 1 - 1% alginic acid, 2% - the bone protein and 0.05% - vitamin PP; group 2 - 20 g of laminaria, group 3 - ferrocin in dose of 0.02 g/rat per day. It was shown, that the most favourable effect was exerted by laminaria not causing the increase of he weight, decorporating both strontiu and caesium, reducing verifiably the content of primary products of lipid peroxidation in the blood. It allows to recommend it for inclusion in the ration of population of radiocontaminated areas.

  3. ABO (histo) blood group phenotype development and human reproduction as they relate to ancestral IgM formation: A hypothesis.

    Science.gov (United States)

    Arend, Peter

    2016-01-01

    The formation of a histo (blood) group) ABO phenotype and the exclusion of an autoreactive IgM or isoagglutinin activity arise apparently in identical glycosylation of complementary domains on cell surfaces and plasma proteins. The fundamental O-glycan emptiness of the circulating IgM, which during the neonatal amino acid sequencing of the variable regions is exerting germline-specific O-GalNAc glycan-reactive serine/threonine residues that in the plasma of the adult human blood group O individuals apparently remain associated with the open glycosidic sites on the ABOH convertible red cell surface, must raise suggestions on a transient expression of developmental glycans, which have been "lost" over the course of maturation. In fact, while the mammalian non-somatic, embryogenic stem cell (ESC)- germ cell (GC) transformation is characterized by a transient and genetically as-yet-undefined trans-species-functional O-GalNAc glycan expression, in the C57BL/10 mouse such expression was potentially identified in growth-dependent, blood group A-like GalNAc glycan-bearing, ovarian glycolipids complementary with the syngeneic anti-A reactive IgM, which does not appear in early ovariectomized animals. This non-somatically encoded, polyreactive, ancestral IgM molecule has not undergone clonal selection and does primarily not differentiate between self and non-self and might, due to amino acid hydroxyl groups, highly suggest substrate competition with subsequent O-glycosylations in ongoing ESC-GC transformations and affecting GC maturation. However, the membrane-bound somatic N/O-glycotransferases, which initiate, after formation of the zygote, the complex construction of the human ABO phenotypes in the trans cisternae of the Golgi apparatus, are associated and/or completed with soluble enzyme versions exerting identical specificities in plasma and likely competing vice versa by glycosylation of neonatal IgM amino acids, where they suggest to accomplish the clearance of anti

  4. Effect of honey vinegar syrup on blood sugar and lipid profile in healthy subjects

    Directory of Open Access Journals (Sweden)

    Seyedeh-Masomeh Derakhshandeh-Rishehri

    2014-01-01

    Full Text Available Background: The impact of honey or vinegar on several metabolic abnormalities has been studied separately, a mixture of these two ingredients known as honey vinegar syrup (HVS has not been investigated previously so far. The aim of this study was to assess the impact of HVS consumption (Iranian′s traditional syrup on glycemic parameters and lipid profiles in healthy individuals. Methods: We conducted a 4-week, randomized, controlled, parallel study consisting of two groups of nonobese healthy volunteers. All subjects were asked to stay on their normal diet. Intervention group (n = 36 received a cup of HVS daily in the evening snack for 4-week (250 cc syrup contains 21.66 g honey vinegar. Assessments of fasting blood sugar (FBS, insulin, homeostasis model assessment of insulin resistance (HOMA-IR, total cholesterol (TC, triglyceride (TG, high-density lipoprotein cholesterol (HDL-C and low-density lipoprotein cholesterol (LDL-C were conducted at the baseline and after 4-week of study. Results: We observed no significant effect of HVS on FBS, HOMA-IR, LDL-C and TG. A significant effect of HVS was found on increasing fasting insulin and HOMA-IR and reduction in TC level only in intervention group (Δ =3.39 P = 0.01, Δ =1.65 P = 0.03, Δ = −9.43 P = 0.005, respectively. Changes of FBS, TG and LDL-C were 1.83 mg/dl, −1.53 mg/dl and − 3.99 mg/dl respectively in the intervention group. These changes were not significant. An unfavorable and significant reduction in HDL-C level was also observed between two groups (Δ = −4.82 P < 0.001 in the intervention group. Conclusions: Honey vinegar syrup increased fasting insulin level and decreased TC level in the intervention group. HVS had an unfavorable effect on HDL-C level. Further prospective investigations are warranted to confirm these findings.

  5. Lipid Replacement Therapy Functional Food Formulation with NT Factor for Reducing Weight, Girth, Body Mass, Appetite and Fatigue While Improving Blood Lipid Profiles

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    Rita R. Ellithorpe

    2012-01-01

    Full Text Available Background: Lipid Replacement Therapy using NT Factor® plus kidney bean alpha-amylase inhibitor (Healthy Curb® was used in a two month weight loss clinical trial to reduce weight and improve fatigue without changing easting or exercise patterns and without use of drugs, stimulants or herbs. Objectives: To determine the effects of an all-natural functional food, NT Factor® plus alpha-amylase inhibitor (Healthy Curb®, on weight loss, body girth, body mass and index, basal metabolic rate, appetite, carvings for sweets and fatigue as well as blood lipid profiles during a 2-month open label clinical trial without food restrictions or increases in physical activity.Methods: Thirty subjects (Mean Age = 56.8 ± 1.8; 24 females and 6 males used the functional food containing NT Factor® (500 mg and alpha-amylase inhibitor (500 mg 30 min before each meal in tablet form. Participants were told to eat and exercise normally. Weight, waist and hip measurements were taken weekly. Appetite and sweet cravings were assessed weekly by standard methods. Fatigue was determined using the Piper Fatigue Scale. Blood samples were taken prior to and at the end of the trial for lipid and chemical analyses. Results: Sixty-three percent of the participants lost an average of 6.11 ± 0.28 pounds (2.77 ± 0.12 Kg (p<0.001 along with average reductions of 2.51 ± 0.05 inches (6.4 ± 0.13 cm (p<0.0001 and 1.5 ± 0.04 inches (3.8 ± 0.10 cm (p<0.0001 from waist and hip circumferences, respectively. The entire Functional Foods in Health and Disease 2012, 2(1:11-24 group lost an average of 3.63 ± 0.13 pounds (1.65 ± 0.11 Kg (p<0.001 with average reductions of 1.59 ± 0.03 inches (4.04 ± 0.06 cm (p<0.0001 and 1.13 ± 0.02 inch (2.87 ± 0.05 cm (p<0.0001 from waist and hip circumferences, respectively. Weight loss and body measurement decreases were gradual, consistent and significant, along with reductions in body mass index (BMI and basal metabolic rate (BMR measurements

  6. The effect of lipid regulation with atorvastatin on the blood lipid levels and carotid artery plaques in patients with atherosclerotic cerebral infarction

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    Shu XU

    2015-11-01

    Full Text Available Objective To analyze the effect of intensive lipid regulation treatment with atorvastatin on the blood lipid levels and carotid artery plaques in patients with atherosclerotic cerebral infarction.  Methods Ninety-two patients with atherosclerotic cerebral infarction were randomly divided into two groups: observation group (treated by atorvastatin calcium with the dosage of 20 mg/d, N = 46 and control group (treated by diet without lipid-rich food, N=46. Besides, other drugs given to the patients in two groups were the same. The blood lipid levels and the changes of carotid artery plaques in two groups were analyzed and compared before treatment and 3 months after treatment. Results After treatment, the concentrations of total cholesterol [TC, (4.23 ± 0.92 mmol/L vs (5.24 ± 0.68 mmol/L], triglyceride [TG, (2.46 ± 0.28 mmol/L vs (3.33 ± 0.47 mmol/L], low-density lipoprotein cholesterol [LDL-C, (2.52 ± 0.38 mmol/L vs (4.78 ± 0.86 mmol/L] in the patients of observation group were all decreased and significantly lower than those in the control group (P = 0.000, for all, and the concentration of high-density lipoprotein cholesterol [HDL-C, (1.13 ± 0.41 mmol/L vs (0.85 ± 0.32 mmol/L] in the patients of observation group was increased and significantly than that in the control group (P = 0.003. The carotid artery plaque size [(20.25 ± 0.32 mm2 vs (24.42 ± 10.33 mm2] and thickness [(0.59 ± 0.13 mm vs (1.93 ± 0.23 mm] of carotid artery plaques and intima?media thickness [IMT, (1.32 ± 0.67 mm vs (1.63 ± 0.56 mm] of common carotid artery (CCA in the patients of observation group were all significantly lower than those in patients in the control group (P = 0.000, 0.000, 0.010, respectively. Comparing serum alanine aminotransferase (ALT, aspartate aminotransferase (AST, creatine kinase (CK and creatinine (Cr levels after treatment with before treatment, there was no significant difference between 2 groups (P > 0.05, for all.  Conclusions

  7. Lipid profile alterations and fasting blood glucose levels in primary hypothyroidism

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    Maram Sushma

    2014-08-01

    Conclusions: Hypothyroid patients demonstrate significant increase in serum lipids as compared to healthy individuals. More females are diagnosed with hypothyroidism and dyslipidemia is associated with primary hypothyroidism. [Int J Res Med Sci 2014; 2(4.000: 1694-1698

  8. Investigation of the effects of a high fish diet on inflammatory cytokines, blood pressure, and lipids in healthy older Australians

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    Jessica A. Grieger

    2014-01-01

    Full Text Available Background: Aging is a condition of chronic inflammation. In healthy Australians ≥64 years, the primary aim was to determine whether four servings/week of mixed fish (FISH improves serum cytokines (i.e. C-reactive protein (CRP, IL-1, IL-6, TNF-α compared to a diet low in fish (<1 serving/week, CONTROL; the secondary aims were to assess the effect of the diet on blood pressure and serum lipids (TC, HDL-C, TG, calculated LDL-C. Methods: An 8-week randomized, parallel study, stratified by CRP (<3 mg/L vs. ≥3 mg/L on entry to the study. Compliance was measured using 3-day weighed food records in weeks 1 and 7 of the study. A 12-h fasting blood sample was taken at baseline and 8-weeks for erythrocyte fatty acids as confirmation of compliance, and measurement of serum cytokines and lipids. Blood pressure was measured at both time points. Results: Eighty participants completed the study (mean (SD age: 69.6 (5.8 years. During week 1 of the study, mean ± SEM daily dietary intake of very long chain omega-3 polyunsaturated fatty acids (VLCN n-3 PUFA in FISH vs. CONTROL was 1,676±129 mg vs. 27±5 mg (p<0.001. Mean (SD gram intake of study fish and meat was 121 (45 g and 123 (78 g, for those allocated to FISH and CONTROL, respectively. Mean ± SEM percentage VLCN n-3 PUFA in erythrocytes at 8-weeks was higher in those allocated to FISH vs. CONTROL (10.2±0.2% vs. 8.2±0.3%, p<0.001. There was no between-group difference in CRP (n=80, IL-1β (n=33 or IL-6 (n=21 concentrations, blood pressure, or lipids, at 8-weeks. Conclusions: Eight weeks consumption of four servings/week fish did not affect serum cytokine concentrations, blood pressure or lipids compared to a diet low in fish. In healthy older adults with low inflammatory burden, our results do not support that short-term consumption of mixed fish has a beneficial effect on selected cardiovascular biomarkers.

  9. The effects of three-week fasting diet on blood pressure, lipid profile and glucoregulation in extremely obese patients

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    Beleslin Biljana

    2007-01-01

    Full Text Available Introduction Obesity is often accompanied by a number of complications including diabetes mellitus and cardiovascular diseases. Elevated blood pressure and lipids, as well as deterioration of glucoregulation are attributed, as the most significant factors, to development of diabetes mellitus and cardiovascular complications in obese patients. Objective The aim of our study was to evaluate the effects of a fasting diet on blood pressure, lipid profile and glucoregulatory parameters. Method We included 110 patients (33 male and 77 female; mean age 35±1 years, body weight 131.7±2.6 kg, body mass index 45.4±0.8 kg/m2 who were hospitalized for three weeks for the treatment of extreme obesity with the fasting diet. At the beginning, during, and at the end of this period, we evaluated changes in blood pressure, lipid profile, as well as parameters of glucoregulation including glycaemia, insulinaemia, and insulin sensitivity by HOMA. Oral glucose tolerance test (OGTT was performed in all patients at the beginning and at the end of the fasting diet. Results During the fasting diet, the body weight decreased from 131.7±2.6 kg to 117.7±2.4 kg (p<0.001, the body mass index decreased from 45.4±0.8 kg/m2 to 40.8±0.8 kg/m2 (p<0.001, and both systolic and diastolic blood pressure significantly declined (143±2 vs. 132±2 mm Hg, p<0.001; 92±2 vs. 85±2 mm Hg, p<0.001. In addition, the fasting diet produced a significant decrease in total cholesterol, LDL cholesterol, triglycerides, as well as basal glycaemia and insulinaemia (p<0.001 Before the fasting diet, OGTT was normal in 76% of patients, whereas 21% of patients showed glucose intolerance, and 4% of patients diabetes mellitus. After the fasting diet, OGTT was normal in 88% of patients, whereas 12% of patients still had signs of glucose intolerance (p<0.05. In addition, insulin resistance significantly (p<0.05 increased from 54±6% to 89±13% after the fasting diet. Conclusion The three-week fasting

  10. Effects of Encapsulated Propolis on Blood Glycemic Control, Lipid Metabolism, and Insulin Resistance in Type 2 Diabetes Mellitus Rats

    Science.gov (United States)

    Li, Yajing; Chen, Minli; Xuan, Hongzhuan; Hu, Fuliang

    2012-01-01

    The present study investigates the encapsulated propolis on blood glycemic control, lipid metabolism, and insulin resistance in type 2 diabetes mellitus (T2DM) rats. The animal characteristics and biological assays of body weight, fasting blood glucose (FBG), fasting serum insulin (FINS), insulin act index (IAI), triglycerides (TG), total cholesterol (TC), high-density lipoprotein cholesterol (HDL-C), and low-density lipoprotein cholesterol (LDL-C) were measured and euglycemic hyperinsulinemic glucose clamp technique were used to determine these effects. Our findings show that oral administration of encapsulated propolis can significantly inhibit the increasing of FBG and TG in T2DM rats and can improve IAI and M value in euglycemic hyperinsulinemic clamp experiment. There was no significant effects on body weight, TC, HDL-C, and LDL-C in T2DM rats treated with encapsulated propolis. In conclusion, the results indicate that encapsulated propolis can control blood glucose, modulate lipid metabolism, and improve the insulin sensitivity in T2DM rats. PMID:21716678

  11. Effects of Encapsulated Propolis on Blood Glycemic Control, Lipid Metabolism, and Insulin Resistance in Type 2 Diabetes Mellitus Rats

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    Yajing Li

    2012-01-01

    Full Text Available The present study investigates the encapsulated propolis on blood glycemic control, lipid metabolism, and insulin resistance in type 2 diabetes mellitus (T2DM rats. The animal characteristics and biological assays of body weight, fasting blood glucose (FBG, fasting serum insulin (FINS, insulin act index (IAI, triglycerides (TG, total cholesterol (TC, high-density lipoprotein cholesterol (HDL-C, and low-density lipoprotein cholesterol (LDL-C were measured and euglycemic hyperinsulinemic glucose clamp technique were used to determine these effects. Our findings show that oral administration of encapsulated propolis can significantly inhibit the increasing of FBG and TG in T2DM rats and can improve IAI and M value in euglycemic hyperinsulinemic clamp experiment. There was no significant effects on body weight, TC, HDL-C, and LDL-C in T2DM rats treated with encapsulated propolis. In conclusion, the results indicate that encapsulated propolis can control blood glucose, modulate lipid metabolism, and improve the insulin sensitivity in T2DM rats.

  12. WATER EXTRACT OF SWEET POTATO LEAF IMPROVED LIPID PROFILE AND BLOOD SOD CONTENT OF RATS WITH HIGH CHOLESTEROL DIET

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    I Wayan Sumardika

    2013-04-01

    Full Text Available Food stuffs with high flavonoids content  are believed to prevent various diseases caused by oxidative stress because of its antioxidants effect.  Purple sweet potato leaves have been proved containing high flavonoids, and can be developed very easily. To prove antioxidant and hypolipidemic properties of the water extract of purple sweet potato leaves, one research was conducted in the Department of Pharmacology, Faculty of Medicine, Udayana University. The study design was randomized control group pre- and post-test.  Twenty adult male wistar rats were divided into two groups of 10 rats. Both groups of rats were given high-cholesterol diet for three months to induce dyslipidemia. Control group of 10 rats were given only high-cholesterol diet alone, whereas the treatment group also treated with purple sweet potato leaf water extract with a dose of 6 cc per day divided into two doses. Before treatment and after treatment, lipid profile and blood SOD levels were measured. The results showed a decrease in total cholesterol, triglycerides and LDL cholesterol significantly in the treated group (P = 0.0001. In the treatment group there was also an increase in HDL cholesterol and blood SOD which was significantly differ than the control group (P = 0.0001. From the results of this study, it can be concluded that administration of water extract of purple sweet potato leaves can improve the lipid profile and increase blood SOD of rat given high-cholesterol diet.

  13. Study on the relationship between red blood cell immunity and lipid peroxidation in patients with hand-foot-mouth disease

    International Nuclear Information System (INIS)

    Objective: To study the relationship between red blood cell immunity and lipid peroxidation in patients with hand-foot-mouth disease. Methods: The red blood cell C3b receptor (RBC-C3bR) and red blood cell immune complex rosette (RBC-ICR) were measured in 42 patients with hand-foot-mouth disease as well as in 35 controls. The serum levels of MDA, SOD and GSH-PX were also measured with colorimetry in these subjects. Results: RBC-ICRR and MDA levels were significantly higher in patients with hand-foot-mouth disease than those in controls(P<0.01), while the RBC-C3bR, SOD, GSH-PX levels were significantly lower in patients with hand-foot-mouth disease than those in controls (P<0.01). RBC-C3bR was negatively correlated with MDA (r=-0.5014, P<0.05), but RBC-ICR was positively correlated with MDA (r=0.5942, P<0.01). Conclusion: The lowered red cell immune function was closely associated with the lipid peroxidation injury in patients with hand-foot-mouth disease. (authors)

  14. Un caso de grupo sanguíneo raro: fenotipo p A rare blood group: p phenotype

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    Carlos D. De La Vega Elena

    2009-12-01

    work-up grouped her as belonging to "p" phenotype, associated with difficulties to find compatible blood for transfusion and a high incidence of recurrent miscarriage. At 36 weeks, a baby girl was born by induced labor due to fetal suffering. With a negative direct antiglobulin test but a positive elution test, she was in the neonatology ward for one week receiving luminotherapy. Homozygosity for a missense mutation at position 752 (c.752C > T in the A4GALT gene was found to be responsible for the p phenotype. This mutation changes a proline to a leucine at codon 251 of the 4-α-galactosyltransferase. Recently, due to an imminent chirurgical intervention and the impossibility to have compatible blood available for transfusion, an autologous donation plan was designed to satisfy probable demand. This case showed the need for blood bank facilities capable to respond satisfactorily to these situations in Argentina. This would facilitate the storage of cryopreserved blood from individuals with rare blood groups for homologous use or to develop rare blood donors programs.

  15. Fatal Cyberlindnera fabianii fungemia in a patient with mixed phenotype acute leukemia after umbilical cord blood transplantation.

    Science.gov (United States)

    Katagiri, Seiichiro; Gotoh, Moritaka; Tone, Kazuya; Akahane, Daigo; Ito, Yoshikazu; Ohyashiki, Kazuma; Makimura, Koichi

    2016-05-01

    We report a case of Cyberlindnera fabianii fungemia after umbilical cord blood transplantation (CBT). A 69-year-old woman was diagnosed as having mixed phenotype acute leukemia. The patient received CBT for primary refractory disease. After preconditioning chemotherapy, the patient's condition deteriorated, leading to acute respiratory failure from capillary leak syndrome and consequent admittance to the intensive care unit. The patient recovered temporarily following the administration of noninvasive positive pressure ventilation and continuous hemodiafiltration, but died of fungemia with the presence of yeast-like cells 15 days post-CBT. The yeast-like cells were analyzed by sequencing of the D1/D2 domain of the large subunit and the internal transcribed spacer domain, and were identified as C. fabianii. This case shows that molecular genetic-based methods may be effective for detecting undetermined invasive fungal infections in stem cell transplantation settings. PMID:26879198

  16. Shwachman-Diamond syndrome presenting with early ichthyosis, associated dermal and epidermal intracellular lipid droplets, hypoglycemia, and later distinctive clinical SDS phenotype.

    Science.gov (United States)

    Scalais, Emmanuel; Connerotte, Anne-Catherine; Despontin, Karine; Biver, Armand; Ceuterick-de Groote, Chantal; Alders, Marielle; Kolivras, Athanassios; Hachem, Jean-Pierre; De Meirleir, Linda

    2016-07-01

    Shwachman-Diamond syndrome (SDS) is a recessive ribosomopathy, characterized by bone marrow failure and exocrine pancreatic insufficiency (ePI) often associated with neurodevelopmental and skeletal abnormalities. The aim of this report is to describe a SDS patient with early ichthyosis associated with dermal and epidermal intracellular lipid droplets (iLDs), hypoglycemia and later a distinctive clinical SDS phenotype. At 3 months of age, she had ichthyosis, growth retardation, and failure to thrive. She had not cytopenia. Ultrasonography (US) showed pancreatic diffuse high echogenicity. Subsequently fasting hypoketotic hypoglycemia occurred without permanent hepatomegaly or hyperlipidemia. Continuous gavage feeding was followed by clinical improvement including ichthyosis and hypoglycemia. After 14 months of age, she developed persistent neutropenia and ePI consistent with SDS. The ichthyotic skin biopsy, performed at 5 months of age, disclosed iLDs in all epidermal layers, in melanocytes, eccrine sweat glands, Schwann cells and dermal fibroblasts. These iLDs were reminiscent of those described in Dorfman-Chanarin syndrome (DCS) or Wolman's disease. Both LIPA and CGI-58 analysis did not revealed pathogenic mutation. By sequencing SBDS, a compound heterozygous for a previously reported gene mutation (c.258 + 2T>C) and a novel mutation (c.284T>G) were found. Defective SBDS may hypothetically interfere as in DCS, with neutral lipid metabolism and play a role in the SDS phenotype such as ichthyosis with dermal and epidermal iLDs and hypoglycemia. This interference with neutral lipid metabolism must most likely occur in the cytoplasm compartment as in DCS and not in the lysosomal compartment as in Wolman's disease. © 2016 Wiley Periodicals, Inc. PMID:27127007

  17. Altered Peripheral Blood Monocyte Phenotype and Function in Chronic Liver Disease: Implications for Hepatic Recruitment and Systemic Inflammation.

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    Victoria L Gadd

    Full Text Available Liver and systemic inflammatory factors influence monocyte phenotype and function, which has implications for hepatic recruitment and subsequent inflammatory and fibrogenic responses, as well as host defence.Peripheral blood monocyte surface marker (CD14, CD16, CD163, CSF1R, CCR2, CCR4, CCR5, CXCR3, CXCR4, CX3CR1, HLA-DR, CD62L, SIGLEC-1 expression and capacity for phagocytosis, oxidative burst and LPS-stimulated TNF production were assessed in patients with hepatitis C (HCV (n = 39 or non-alcoholic fatty liver disease (NAFLD (n = 34 (classified as non-advanced disease, compensated cirrhosis and decompensated cirrhosis and healthy controls (n = 11 by flow cytometry.The selected markers exhibited similar monocyte-subset-specific expression patterns between patients and controls. Monocyte phenotypic signatures differed between NAFLD and HCV patients, with an increased proportion of CD16+ non-classical monocytes in NAFLD, but increased expression of CXCR3 and CXCR4 in HCV. In both cohorts, monocyte CCR2 expression was reduced and CCR4 elevated over controls. CD62L expression was specifically elevated in patients with decompensated cirrhosis and positively correlated with the model-for-end-stage-liver-disease score. Functionally, monocytes from patients with decompensated cirrhosis had equal phagocytic capacity, but displayed features of dysfunction, characterised by lower HLA-DR expression and blunted oxidative responses. Lower monocyte TNF production in response to LPS stimulation correlated with time to death in 7 (46% of the decompensated patients who died within 8 months of recruitment.Chronic HCV and NAFLD differentially affect circulating monocyte phenotype, suggesting specific injury-induced signals may contribute to hepatic monocyte recruitment and systemic activation state. Monocyte function, however, was similarly impaired in patients with both HCV and NAFLD, particularly in advanced disease, which likely contributes to the increased

  18. Smooth Muscle Cell Alignment and Phenotype Control by Melt Spun Polycaprolactone Fibers for Seeding of Tissue Engineered Blood Vessels

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    Animesh Agrawal

    2015-01-01

    Full Text Available A method has been developed to induce and retain a contractile phenotype for vascular smooth muscle cells, as the first step towards the development of a biomimetic blood vessel construct with minimal compliance mismatch. Melt spun PCL fibers were deposited on a mandrel to form aligned fibers of 10 μm in diameter. The fibers were bonded into aligned arrangement through dip coating in chitosan solution. This formed a surface of parallel grooves, 10 μm deep by 10 μm across, presenting a surface layer of chitosan to promote cell surface interactions. The aligned fiber surface was used to culture cells present in the vascular wall, in particular fibroblasts and smooth muscle cells. This topography induced “surface guidance” over the orientation of the cells, which adopted an elongated spindle-like morphology, whereas cells on the unpatterned control surface did not show such orientation, assuming more rhomboid shapes. The preservation of VSMC contractile phenotype on the aligned scaffold was demonstrated by the retention of α-SMA expression after several days of culture. The effect was assessed on a prototype vascular graft prosthesis fabricated from polylactide caprolactone; VSMCs aligned longitudinally along a fiberless tube, whereas, for the aligned fiber coated tubes, the VSMCs aligned in the required circumferential orientation.

  19. PHENOTYPIC AND GENOTYPIC CHARACTERIZATION OF MDR ISOLATES OF STAPHYLOCOCCUS AUREUS FROM BLOOD AND PUS IN HIMACHAL PRADESH, INDIA

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    Neha Sharma

    2012-08-01

    Full Text Available Drug resistance in Staphylococcus aureus with variable epidemiology has become a serious public health problem worldwide. To study the phenotype prevalent in this region (Himachal Pradesh and to choose the appropriate antibiotic therapy regimen both phenotypic and genotypic characterization of the given isolates were performed in the present work. A total 25 isolates (20 of pus and 5 of blood were obtained from IGMC Shimla and their antibiotic resistance profiling was done. 100% resistance was obtained in case of tetracycline and 48% for oxacillin. Vancomycin, netilmycin, teicoplanin and linezolid, showed 100% sensitivity where as in case of clindamycin 88% sensitivity was seen as three of the isolates were found to be intermediate. Genotypic characterization of both MSSA and MRSA strains (5 each was done for the presence of tet 38 gene by PCR. The results of PCR revealed all the 10 isolates carrying tet 38 gene with amplicon size of 1353 base pair irrespective of whether the isolates were MSSA or MRSA.

  20. The correlation of the serum leptin, insulin resistance, endocrine hormone, blood lipid and of simple obesity children and growth

    Institute of Scientific and Technical Information of China (English)

    Xiao-Yu Wang; Xiao-Yun Liu; Kai-Yu Xu

    2015-01-01

    Objective:To analyze the correlation of the serum leptin, insulin resistance, Endocrine hormone, blood lipid and of simple obesity children and growth.Methods:200 cases of simple obesity children (Study Group) and 200 cases of non obese children (control group) were studied, Fasting blood glucose(FBG), Glycosylated hemoglobin (HbAlc), the serum leptin detection (Leptin), insulin (INS), insulin resistance index (HOMA2-IR), growth hormone (GH), cortisol (cortisol), thyroid hormones and blood lipid indexes were detected.Results:Leptin, cortisol and T3 levels of study group were (21.39±2.29) g/L, (18.43±1.72) g/mL and (20.71±1.82) pmol/L, were significantly increased compared with the control group, and GH level was (3.15±0.41) g/L, was significantly reduced, the difference of all these indexes were statistically significant in two groups (P0.05), HbAlc, INS and HOMA2 - IR were respectively (5.82±0.87)%, (33.02-2.34) mIU/L, (3.31-0.54), compared with the control group obviously increase than the corresponding numerical of the control group, the difference of all these indexes have statistical significance in two groups (P<0.05). TC, TG and LDL-C of study group were (4.67±0.65) mmol/L, (1.62±0.45) mmol/L and (3.59±0.46) mmol/L, were significantly higher than those in the control group, while HLD-C was (1.10±0.24) mmol/L, was significantly lower (P<0.05). Conclusion:Children with simple obesity have leptin resistance, reduction of growth hormone and endocrine and lipid metabolism disorder, and seriously affect the normal growth and development of children.

  1. Influence of Concurrent Obesity and Low Birth Weight on Blood Pressure Phenotype in Youth

    OpenAIRE

    Lurbe i Ferrer, Empar; Carvajal Roca, María Eva; Torro Domenech, Maria Isabel; Aguilar, Francisco; Alvarez, Julio; Redón i Más, Josep

    2009-01-01

    The aim of this study was to assess the impact of obesity and low birth weight on both office and ambulatory blood pressure (BP) values, as well as on aortic-derived parameters in youths. A total of 422 white youths, from 10 to 18 years of age, were included. Subjects were divided into 4 groups according to the presence (234; 55%) or the absence (188; 45%) of obesity and according to low (114; 27%) or normal (308; 73%, birth weight. Spacelabs 90207 was used to measure ambulatory B...

  2. Effects of Acupuncture on Hemorheology,Blood Lipid Content and Nail Fold Microcirculation in Multiple Infarct Dementia Patients

    Institute of Scientific and Technical Information of China (English)

    刘清国

    2004-01-01

    Forty-six cases of multiple infarct dementia (MID) in the treatment group were treated by acupuncture with the principle of supplementing the inferiority to clear the superiority and regulating spirit to invigorate intelligence. Changes of the blood lipid content, hemorheological indexes and nail fold microcirculation in the treatment group were compared with those in the randomly assigned control group.The data collected showed that the changes in the treatment group were remarkable, and part of them were superior to their counterparts obtained in the control group by statistical analysis. It is indicated that acupuncture can effectivelv regulate the affected hemodvnamic state in MID.

  3. Effect of regular swimming exercise on the physical composition, strength, and blood lipid of middle-aged women

    OpenAIRE

    Lee, Bo-Ae; Oh, Deuk-Ja

    2015-01-01

    The purpose of this study is to define the effects of regular swimming exercise on the physical composition, physical strength, and blood lipids of middle-aged women. The subjects of this study are a total of 24 middle-aged women in the swimming group and the control group, with 12 women for each group. The swimming group performed swimming exercise for 60 min every time for 3 times a week, for a total of 12 weeks. For data processing, SPSS 21.0 statistics program was used to calculate the me...

  4. Effect of Hydroalcoholic Extract of Capparis Spinosa Fruit on Blood Sugar and Lipid Profile of Diabetic and normal Rats

    OpenAIRE

    Rasool Rahmani; Mehdi Mahmoodi; Mehrnaz Karimi; Fateme Hoseini; Rahim Heydari; Mohamadhosein Salehi; Aliakbar Yousefi

    2013-01-01

    Background: Diabetes mellitus is a common disorder of endocrine glands worldwide. Caper as a medicinal plant has anti-oxidant properties and has been used traditionally to cure diabetes. The aim of present research was to evaluate the effect of 200 and 800 mg/kg of caper fruit extract on blood sugar, glycated hemoglobin and lipid profile in diabetic and normal male rats.Materials & Methods: In this experimental study 60 rats were divided into 6 groups randomly, in which three diabetic groups ...

  5. Effects of Aqua Aerobics on Body Composition, Body Mass, Lipid Profile, and Blood Count in Middle-Aged Sedentary Women

    OpenAIRE

    Kantyka Joanna; Herman Damian; Roczniok Robert; Kuba Lidia

    2015-01-01

    Purpose. The aim of the present investigations was to determine the effects of aqua aerobics on body weight and composition, lipid profile, and selected blood count parameters in middle-aged sedentary females. Methods. Twenty-one women were randomly assigned to an experimental group (age 56.20 ± 2.57 years, height 162.80 ± 4.76 cm, weight 74.03 ± 3.84 kg) that participated in aqua aerobics classes three times a week for three months and a control group (mean age 56.44 ± 3.28 years, height 165...

  6. Short communication: Cytokine profiles from blood mononuclear cells of dairy cows classified with divergent immune response phenotypes.

    Science.gov (United States)

    Martin, C E; Paibomesai, M A; Emam, S M; Gallienne, J; Hine, B C; Thompson-Crispi, K A; Mallard, B A

    2016-03-01

    Genetic selection for enhanced immune response has been shown to decrease disease occurrence in dairy cattle. Cows can be classified as high (H), average, or low responders based on antibody-mediated immune response (AMIR), predominated by type-2 cytokine production, and cell-mediated immune response (CMIR) through estimated breeding values for these traits. The purpose of this study was to identify in vitro tests that correlate with in vivo immune response phenotyping in dairy cattle. Blood mononuclear cells (BMC) isolated from cows classified as H-AMIR and H-CMIR through estimated breeding values for immune response traits were stimulated with concanavalin A (ConA; Sigma Aldrich, St. Louis, MO) and gene expression, cytokine production, and cell proliferation was determined at multiple time points. A repeated measures model, which included the effects of immune response group, parity, and stage of lactation, was used to compare differences between immune response phenotype groups. The H-AMIR cows produced more IL-4 protein than H-CMIR cows at 48h; however, no difference in gene expression of type-2 transcription factor GATA3 or IL4 was noted. The BMC from H-CMIR cows had increased production of IFN-γ protein at 48, 72, and 96h compared with H-AMIR animals. Further, H-CMIR cows had increased expression of the IFNG gene at 16, 24, and 48h post-treatment with ConA, although expression of the type-1 transcription factor gene TBX21 did not differ between immune response groups. Although proliferation of BMC increased from 24 to 72h after ConA stimulation, no differences were found between the immune response groups. Overall, stimulation of H-AMIR and H-CMIR bovine BMC with ConA resulted in distinct cytokine production profiles according to genetically defined groups. These distinct cytokine profiles could be used to define disease resistance phenotypes in dairy cows according to stimulation in vitro; however, other immune response phenotypes should be assessed. PMID

  7. Influence of a hyperlipidic diet on the composition of the non-membrane lipid pool of red blood cells of male and female rats.

    Science.gov (United States)

    Remesar, Xavier; Antelo, Arantxa; Llivina, Clàudia; Albà, Emma; Berdié, Lourdes; Agnelli, Silvia; Arriarán, Sofía; Fernández-López, José Antonio; Alemany, Marià

    2015-01-01

    Background and objectives. Red blood cells (RBC) are continuously exposed to oxidative agents, affecting their membrane lipid function. However, the amount of lipid in RBCs is higher than the lipids of the cell membrane, and includes triacylglycerols, which are no membrane components. We assumed that the extra lipids originated from lipoproteins attached to the cell surface, and we intended to analyse whether the size and composition of this lipid pool were affected by sex or diet. Experimental design. Adult male and female Wistar rats were fed control or cafeteria diets. Packed blood cells and plasma lipids were extracted and analysed for fatty acids by methylation and GC-MS, taking care of not extracting membrane lipids. Results. The absence of ω3-PUFA in RBC extracts (but not in plasma) suggest that the lipids extracted were essentially those in the postulated lipid surface pool and not those in cell membrane. In cells' extracts, there was a marked depletion of PUFA (and, in general, of insaturation). Fatty acid patterns were similar for all groups studied, with limited effects of sex and no effects of diet in RBC (but not in plasma) fatty acids. Presence of trans fatty acids was small but higher in RBC lipids, and could not be justified by dietary sources. Conclusions. The presence of a small layer of lipid on the RBC surface may limit oxidative damage to the cell outer structures, and help explain its role in the transport of lipophilic compounds. However, there may be other, so far uncovered, additional functions for this lipid pool. PMID:26213652

  8. Phenotype of blood lymphocytes in correlation with histological picture in thyroid gland of rats treated with potassium iodide

    Directory of Open Access Journals (Sweden)

    Marković Ljiljana

    2011-01-01

    Full Text Available Having in mind the former results which confirmed the functional relationship between the thyroid and the immune system, and the administration of potassium iodide (KI in the therapy of auto-immune diseases, we considered it of interest to investigate the pathogenesis of KI induced experimental thyroiditis, by studying the morphology of thymus, thyroid gland and spleen, as well as the phenotype of lymphocytes in the thymus and peripheral blood in relation to the expression of CD4 and CD8 molecules. The experiments have been carried out on 30 male Wistar rats, divided in 3 groups. The first group (n=10 received KI (225 μg/g, i.p., the second group (n=10 received KI (675 μg/g, i.p., while the third group (n=10 received sodium chloride (0.9%, i.p.. The intensity of histological lesions in the thyroid gland, was statistically significant (p<0.01 in KI treated groups, compared to the controls. KI also significantly decreased rat body mass, and increased masses of thymus and thyroid gland (p<0.05. No statistically significant difference was found in thymocyte and peripheral blood CD4+ and CD8+ subpopulation numbers between the groups. Our experiments suggest that KI, at least in some doses could induce mild lymphocytic thyroiditis in rats, and that this simple, practical and non-expensive model of experimental thyroiditis could be of importance for further research.

  9. CORRELATION OF PLASMA FIBRINOGEN WITH BLOOD PRESSURE, BMI, LIPID PROFILE AND GLYCEMIC STATUS IN TYPE II D M

    Directory of Open Access Journals (Sweden)

    Suresh

    2014-12-01

    Full Text Available BACKGROUND: Diabetes is the most common metabolic disorder all over the world3.The incidence of diabetes is showing an alarming rise in developing countries, particularly in India. Type 2 diabetes is the most prevalent form in India and constitutes more than 95% of the cases.9 During the past decade, the potential role of haemostatic factors particularly fibrinogen in various disorders and their complication has gained considerable interest. Plasma fibrinogen is a important marker in type 2 Diabetes, but its correlation with smoking, age, sex, hypertension, obesity, family history lipids, has not been evaluated in large studies. In view of paucity of data from Indian studies, we attempt to correlate plasma fibrinogen with blood pressure, BMI, lipid profile and glycemic status in type 2 diabetes mellitus. METHODS: A total of 100, known and newly detected type-2 diabetic patients with and without associated hypertension of more than 40 years of age belonging to both sexes were included. All these patients were registered cases in Basaveshwar Teaching & General Hospital, Gulbarga as outpatients and in-patients. Type-2 diabetic patients associated with myocardial infarction, stroke, chronic inflammatory diseases, tuberculosis, malignancy, secondary hypertension and pregnancy were excluded from this study. After a detailed clinical examination, the following investigations were done: For diabetes mellitus: Random blood sugar, fasting blood sugar, post-prandial blood sugar and glycosylated haemoglobin (ERBA Kit. Renal profile: Blood urea and serum creatinine. Serum lipids: Cholesterol, triglyceride (TG, high density lipoprotein (HDL and low density lipoprotein (LDL by commercially available kits (ERBA Kit. Routine urine examination: Sugar and albumin. Estimation of plasma fibrinogen: The plasma fibrinogen was estimated by thrombin-clotting method by using FIBROQUANT KIT [Tulip Diagnostics (P Ltd.]. OBJECTIVES: To know the fibrinogen levels in patients

  10. Mediterranean diet and insulin sensitivity, lipid profile and blood pressure levels, in overweight and obese people; The Attica study

    Directory of Open Access Journals (Sweden)

    Zampelas Antonis

    2007-09-01

    Full Text Available Abstract Background We aimed to investigate if overweight and obese adults "close" to Mediterranean diet present better insulin, lipids profile and better pressure levels, compared to individuals close to a more Westernized diet. Methods The ATTICA study is a population-based cohort that has randomly enrolled 3042 adult men and women, stratified by age – gender, from the greater area of Athens, during 2001–2002. Of them, in this work were have studied 1762 participants with excess body weight, meaning overweight (BMI: 25–29.9 kg/m2 and obese (BMI>30 kg/m2. 1064 were men and 698 women (20–89 years old. Adherence to Mediterranean diet was assessed through a diet-score that was based on a validated food-frequency questionnaire. Blood pressure was measured and also fasting glucose, insulin and blood lipids. Insulin sensitivity was also assessed by the homeostasis model assessment (HOMA approach (glucose × insulin/22.5. Results Individuals with excess bodyweight in the highest tertile of diet score, were more insulin sensitive than those in the lowest tertile (11.4% lower HOMA, p = 0.06, had 13% lower levels of total cholesterol (p = 0.001 and 3 mmHg decrease of systolic blood pressure levels (p Conclusion Adherence to Mediterranean diet is modeslty associated with a better insulin sensitivity, lower levels of total cholesterol and lower levels of systolic blood pressure in overweight and obese subjects. This may suggest that compared to general population, the beneficial effect of this diet in cardiovascular system of excess body weight people is limited.

  11. The effect of two beta-adrenoceptor blockers (mepindolol and atenolol) on blood lipids and platelet aggregation in normal volunteers and essential hypertensive patients.

    OpenAIRE

    Luque Otero, M; Fernandez Pinilla, C; Escriba Polo, A; Rodriguez Vazquez, M; Martell Claros, N; A Fernandez-Cruz

    1984-01-01

    beta-adrenoceptor blocking agents are widely used as treatment of essential hypertension. We studied the action of a new non cardioselective beta-adrenoceptor blocker, mepindolol, comparing its effects on blood pressure, blood lipids and platelet aggregation with those of a cardioselective beta-adrenoceptor blocker, atenolol. Blood pressure fell significantly in the patients treated with both drugs. Triglycerides rose non-significantly only in volunteers treated with mepindolol. We did not fi...

  12. Copper-induced changes of lipid peroxidation and hemato-biochemical parameters in rat blood: Protective role of flavonoids

    Directory of Open Access Journals (Sweden)

    Mladenović Jelena M.

    2014-01-01

    Full Text Available The effects of subchronic exposure to copper (Cu on lipid peroxidation, hemato-biochemical parameters, and the possible protective role of flavonoids Quercetin and (--Epicatechin were studied. Male Wistar albino rats were treated with Cu (560 mg/L, p.o. as CuCl2•2H2O for 5 weeks and Quercetin and (--Epicatechin (40 mg/kg BW each, i.p., every third day during the last 3 weeks alone or in combination. Cu increased the concentration of lipid peroxides, decreased the number of erythrocytes, hemoglobin and hematocrit values and increased the activities of aspartate aminotransferase, alanine aminotransferase and lactate dehydrogenase. Coadministration of Quercetin and (--Epicatechin with Cu lowered the process of lipid peroxidation and restored examined hemato-biochemical parameters to control values. Our results indicate that Cu induced oxidative damage in erythrocytes, which led to anemia, while Quercetin and (--Epicatechin showed a protective effect on the hemato-biochemical processes in the blood of rats. [Projekat Ministarstva nauke Republike Srbije, br. 173041

  13. Influence of blood pressure profile on frailty phenotype in community-dwelling elders in Brazil - FIBRA study.

    Science.gov (United States)

    Fattori, A; Santimaria, M R; Alves, R M A; Guariento, M E; Neri, A L

    2013-01-01

    Frailty is a clinical condition associated with pathological aging and biological vulnerability. In the spectrum of events related to frailty, aging of the cardiocirculatory system and abnormalities in arterial blood pressure (BP) partly explain the changes in tissue perfusion and, potentially, the decrease in physiological reserves. This study investigated the relationship between BP levels, systemic arterial hypertension (SAH) and the frailty phenotype by analyzing frailty criteria in a cross-sectional model into the FIBRA network, a populational sample of community-dwelling elders in Southeastern Brazil. Study participants with ≥65 years were selected by probabilistic sampling of residents in the urban area of the municipality of Campinas (n=900). Considering frailty as a whole and the difference between genders, there was a greater proportion of frail or pre-frail individuals among women than men. Analysis of individual frailty criteria showed that weight loss and fatigue were more common among women (18.3% vs. 12.5%, p=0.034 and 22.5% vs. 11.9%, p<0.001, respectively). Comparison of individuals with or without SAH failed to reveal any differences related to frailty criteria. Nevertheless, averages of diastolic blood pressure (DBP) and mean arterial blood pressure values were lower among elderly individuals with reduced grip strength, physical activity and the frailty classification as a whole (OR 0.986, IC 0.975-0.997) (for every 1 mmHg reduction in MBP values, the likelihood of being frail increased 1.4%). Our findings corroborate the relationship between BP values and frailty in the elderly and contribute to an understanding of the pathophysiological mechanisms of the syndrome. PMID:22939428

  14. Biological, clinical and population relevance of 95 loci for blood lipids

    DEFF Research Database (Denmark)

    Teslovich, Tanya M; Musunuru, Kiran; Smith, Albert V;

    2010-01-01

    in three non-European populations (East Asians, South Asians and African Americans). Our results identify several novel loci associated with plasma lipids that are also associated with CAD. Finally, we validated three of the novel genes-GALNT2, PPP1R3B and TTC39B-with experiments in mouse models. Taken...

  15. Effects of different doses of testosterone on gonadotropins, 25-hydroxyvitamin D3, and blood lipids in healthy men

    Directory of Open Access Journals (Sweden)

    Gårevik N

    2014-12-01

    Full Text Available Nina Gårevik, Anders Rane, Linda Björkhem-Bergman, Lena Ekström Division of Clinical Pharmacology, Department of Laboratory Medicine, Karolinska Institutet, Karolinska University Hospital, Stockholm, Sweden Aims: To study the effect and time profile of different doses of testosterone enanthate on the blood lipid profile and gonadotropins. Experimental design: Twenty-five healthy male volunteers aged 27–43 years were given 500 mg, 250 mg, and 125 mg of testosterone enanthate as single intramuscular doses of Testoviron® Depot. Luteinizing hormone (LH, follicle-stimulating hormone (FSH, blood lipid profile (total cholesterol, plasma [p-] low-density lipoprotein, p-high-density lipoprotein [HDL], p-apolipoprotein A1 [ApoA1], p-apolipoprotein B, p-triglycerides, p-lipoprotein(a, serum [s-] testosterone, and 25-hydroxyvitamin D3 were analyzed prior to, and 4 and 14 days after dosing. Testosterone and epitestosterone in urine (testosterone/epitestosterone ratio were analyzed prior to each dose after a washout period of 6–8 weeks. Results and discussion: All doses investigated suppressed the LH and FSH concentrations in serum. LH remained suppressed 6 weeks after the 500 mg dose. These results indicate that testosterone has a more profound endocrine effect on the hypothalamic–pituitary–gonadal axis than was previously thought. There was no alteration in 25-hydroxyvitamin D3 levels after testosterone administration compared to baseline levels. The 250 and 500 mg doses induced decreased concentrations of ApoA1 and HDL, whereas the lowest dose (125 mg did not have any effect on the lipid profile. Conclusion: The single doses of testosterone produced a dose-dependent increase in serum testosterone concentrations together with suppression of s-LH and s-FSH. Alterations in ApoA1 and HDL were observed after the two highest single doses. It is possible that long-time abuse of anabolic androgenic steroids will lead to alteration in vitamin D status

  16. Impact of single-dose nandrolone decanoate on gonadotropins, blood lipids and HMG CoA reductase in healthy men.

    Science.gov (United States)

    Gårevik, N; Börjesson, A; Choong, E; Ekström, L; Lehtihet, M

    2016-06-01

    The aim was to study the effect and time profile of a single dose of nandrolone decanoate (ND) on gonadotropins, blood lipids and HMG CoA reductase [3-hydroxy-3-methyl-glutaryl-CoA reductase (HMGCR)] in healthy men. Eleven healthy male participants aged 29-46 years were given a single dose of 150 mg ND as an intramuscular dose of Deca Durabol®, Organon. Blood samples for sex hormones, lipids and HMGCR mRNA analysis were collected prior to ND administration day 0, 4 and 14. A significant suppression of luteinising hormone (LH) and follicle-stimulating hormone (FSH) was seen after 4 days. Total testosterone and bioavailable testosterone level decreased significantly throughout the observed study period. A small but significant decrease in sexual hormone-binding globulin (SHBG) was seen after 4 days but not after 14 days. Total serum (S)-cholesterol and plasma (P)-apolipoprotein B (ApoB) increased significantly after 14 days. In 80% of the individuals, the HMGCR mRNA level was increased 4 days after the ND administration. Our results show that a single dose of 150 mg ND increases (1) HMGCR mRNA expression, (2) total S-cholesterol and (3) P-ApoB level. The long-term consequences on cardiovascular risk that may appear in users remain to be elucidated. PMID:26370185

  17. Treatment goals for ambulatory blood pressure and plasma lipids after stroke are often not reached

    DEFF Research Database (Denmark)

    Engberg, Aase Worså; Kofoed, Klaus

    2013-01-01

    In Danish health care, secondary prevention after stroke is currently handled mainly by general practitioners using office blood pressure (OBP) assessment of hypertension. The aim of this study was to compare the OBP approach to 24-hour assessment by ambulatory blood pressure (ABP) monitoring...

  18. Relation of electrocardiographic left ventricular hypertrophy to blood pressure, body mass index, serum lipids and blood sugar levels in adult Nigerians.

    Science.gov (United States)

    Opadijo, O G; Omotoso, A B O; Akande, A A

    2003-12-01

    Left ventricular hypertrophy (LVH) is considered an independent risk factor even in the absence of systemic hypertension. Electrocardiographic (ECG) LVH with repolarisation changes has been found in some countries to carry more coronary risk than LVH alone. How far this observation is true among adult Nigerians is not known. We therefore decided to study adult Nigerians with ECG-LVH with or without ST-T waves changes and compare them with normal age matched controls (without ECG-LVH) in relation with established modifiable risk factors such as systemic hypertension (BP), body mass index (BMI), fasting blood sugar (FBS) and serum lipids such as total cholesterol (Tc), low density lipoprotein cholesterol (LDL-C), high density lipoprotein cholesterol (HDL-C) and triglyceride (TG). Adult Nigerians who were consecutively referred to the ECG laboratory were randomly recruited. Three hundred patients were studied. Their blood pressures (BP) as well as body mass indices were recorded after recording their resting 12 read ECG using portable Seward 9953 ECG machine. Their waist-hip ratio (WHR) was also recorded. Blood samples were taken to determine their fasting blood sugar and serum lipids. Their ECG tracings were read by the cardiologists involved in the study while the blood samples were analysed by the chemical pathologist also involved in the study. At the end of the ECG reading, the patients were divided into 3 groups according to whether there was no ECG-LVH (control group A), ECG-LVH alone (group B), and ECG-LVH with ST-T waves changes (group C). One hundred and fifty (50%) patients belonged to group A, 100 (33.3%) patients to group B and 50 (16.7%) group C. Group B patients were found to have higher modifiable risk factors in form of systemic BP. Tc, LDL-C, and WHR compared to group A. However, the group C patients had increased load of these coronary risk factors in terms of BP elevation, higher BMI, FBS, and scrum cholesterol compared to group B. In addition

  19. Effect of radiation therapy on lipid peroxidation and total antioxidant capacity of blood and saliva in oral cancer patients

    International Nuclear Information System (INIS)

    Radiation therapy is reported to induce oxidative stress in oral cancer patients. Saliva as a diagnostic tool has received increasing attention in recent years. Saliva analysis is proposed to be a noninvasive, sensitive tool for the evaluation of biological effects of radiation therapy in oral cancer. We aimed to assess the effect of radiation therapy on malondialdehyde, the marker of lipid peroxidation, and total antioxidant capacity in blood and saliva of oral cancer patients. We also aimed to assess the correlation between blood and saliva with respect to malonaldehyde (MDA) level and total antioxidant capacity (TAC). Thirty, clinically diagnosed oral cancer patients visiting the Oncology Department were the subjects. Thirty age- and sex-matched normal, healthy controls were included. Blood and saliva samples were collected from controls, and from oral cancer patients before and after radiation therapy. The samples were analyzed for MDA and TAC by standard spectrophotometric methods. Oral cancer patients showed significantly higher MDA level and lower TAC in blood and saliva when compared to controls. One week after radiation therapy, there was significant increase in MDA and decrease in TAC in oral cancer patients. After the completion of radiation therapy of six weeks, MDA level decreased and TAC increased, restoring the values near-to-controls. The pattern of change in MDA and TAC was similar between blood and saliva. There was significant correlation between blood and saliva with respect to MDA and TAC in oral cancer patients. Oral cancer patients showed increased oxidative stress and impaired antioxidant capacity. After radiation therapy of one week, oxidative stress increased further, and after six weeks of radiation therapy there was amelioration of antioxidant status. Saliva could be a sensitive and convenient laboratory tool for diagnosis of oral cancer and evaluation of biological effects of radiation therapy. (author)

  20. Role of hormones and blood lipids in the pathogenesis of acne vulgaris in non-obese, non-hirsute females

    Directory of Open Access Journals (Sweden)

    Ola Ahmed Bakry

    2014-01-01

    Full Text Available Context: Acne vulgaris (AV is a common disease affecting all ages and ethnic groups. Androgens, skin and serum lipids, inflammatory signaling and regulatory neuropeptides seem to be involved in this multi-factorial process. Aim: The aim of this work was to determine hormonal levels and lipid profile in non-obese, non-hirsute females with AV. Subjects and Methods: A total of 60 non-obese, non-hirsute female cases with different grades of AV and 60 age- and gender-matched healthy volunteers were included. Measurement of serum total and free testosterone, sex hormone binding globulin (SHBG, estradiol and progesterone and blood lipids was done during the luteal phase of the menstrual cycle. Results: Total testosterone, free testosterone (FT and progesterone levels were significantly higher (P < 0.001 for all while estradiol levels (P < 0.001 and SHBG (P < 0.01 were significantly lower in cases than controls. Total cholesterol and low density lipoprotein cholesterol (LDL-C levels were significantly higher (P < 0.001 for both while high density lipoprotein cholesterol (HDL-C and apolipoprotein A-1 (ApoA-1 levels were significantly lower (P < 0.001 for both in cases than controls. Higher values of FT (P = 0.03 and SHBG (P = 0.02 and lower values of estradiol (P = 0.04 levels were significantly in favor of severe acne. Higher values of cholesterol (P < 0.001 and LDL-C (P = 0.03 and lower values of HDL-C (P = 0.01 and ApoA-1 (P = 0.02 levels were significantly associated with severe acne. Conclusion: Changes in hormone levels and lipid profile in non-obese and non-hirsute females with AV should be considered in disease pathogenesis and in treatment prescription of these patients.

  1. Quality of the blood pressure phenotype in the GEnotipo, Fenotipo y Ambiente de la hipertensión arterial en UruguaY (GEFA-HT-UY) study

    OpenAIRE

    Luzardo, Leonella; Sottolano, Mariana; Lujambio, Inés; Robaina, Sebastián; Thijs, Lutgarde; da Rosa, Alicia; Krul, Nadia; Carusso, Florencia; Ríos, Ana C; Olascoaga, Alicia; Noboa, Oscar; Staessen, Jan A.; Boggia, José

    2014-01-01

    In the ongoing GEnotipo, Fenotipo y Ambiente de la HiperTensión Arterial en UruguaY (GEFA-HT-UY) study, we applied standardized epidemiological methods to determine complex phenotypes including blood pressure (BP). In this report, we present the quality control of the conventionally measured BP.

  2. [PHENOTYPE OF PERIPHERAL BLOOD NEUTROPHILS IN THE INITIAL STAGE OF ENDOMETRIAL CANCER].

    Science.gov (United States)

    Abakumova, T V; Antoneeva, I I; Gening, T P; Dolgova, D R; Gening, S O

    2016-01-01

    We have examined peripheral blood neutrophils from 123 patients with primary endometrial cancer at stage Ia. Receptor system and the ability of neutrophils to form extracellular traps were assessed by fluorescence microscopy, the spontaneous production of cytokines IL-2, IFN-γ, g-CSF, matrix metalloproteinases-1,9,13 by the method of enzyme-linked immunosorbent assay, phagocytic activity, myeloperoxidase activity, the level of cationic proteis activity in NBT-test were evaluated by cytochemical methods, activity of neutrophils in the spontaneous NBT-test was used to evaluate the oxygen-dependent bactericidal action of neutrophils. The topology and the rigidity of the membrane of neutrophils were assessed by scanning probe microscopy. We have shown that the increase in the relative number of neutrophils lead to a change in their receptor system, aerobic and anaerobic cytotoxicity and ability to phagocytosis are enchanced while reducing NET-activity. We have observed a change in the secretory activity of neutrophils, which is characterized by increased level of MMP-1, possibly initiated by enhanced production of reactive oxygen species, by a reduction in the IL-2 level (inductor of cytotoxic activity) and a sharp increase in the level of the G-CSF. Architectonics of neutrophils in the case of endonetrial cancer at stage Ia is characterized by changing the shape and loss of grit. The rigidity of the cell membrane decreased. Changes in the morphology of neutrophils on the background of the continuing hyperactivity suggests that a state of balance between the immune system and the tumor is already in stage Ia endometrial cancer. PMID:27220248

  3. Lipid peroxidation in rat blood following a single administration of different active 131I

    International Nuclear Information System (INIS)

    Peroxidation in rat blood male Wistar by chemiluminescence single ingestion of radioactive iodine was investigated. Introduction of activity (3.3, 19.2 and 90 kBq per animal) cause changes in the indices of the chemiluminescent reaction (sum light emission, the final intensity of the glow, the time to reach maximum values), the value of which is not significantly dependent on the administration of the active isotope was shown. There were no significant changes in catalase activity in the blood. The features of the flow processes of peroxide in the blood of rats with single administration 131I were discussed

  4. Effects of soy supplementation on blood lipids and arterial function in hypercholesterolaemic subjects

    DEFF Research Database (Denmark)

    Hermansen, K; Hansen, B; Jacobsen, R;

    2005-01-01

    BACKGROUND: Studies on soy supplementation suggest a cardioprotective potential. OBJECTIVE: To examine the effects on LDL cholesterol and arterial function as a result of dietary enrichment with soy supplementation. DESIGN: A Randomized, double blind, parallel intervention trial. SETTING......: Subjects were randomly assigned to 24 weeks of daily intake of either a soy supplement, Abalon (30 g soy protein, 9 g cotyledon fibre and 100 mg isoflavones) or placebo (30 g of casein). The soy supplement and placebo were provided in two sachets daily that were stirred in water. Fasting plasma lipids, TNF...... in 32 subjects, arterial dilatory capacity, compliance, and distensibility were recorded before and after the intervention. RESULTS: In the main study, no difference in fasting plasma lipid levels or insulin sensitivity was found between soy-based supplement and placebo. A significant postprandial...

  5. Genome sequencing elucidates Sardinian genetic architecture and augments association analyses for lipid and blood inflammatory markers.

    Science.gov (United States)

    Sidore, Carlo; Busonero, Fabio; Maschio, Andrea; Porcu, Eleonora; Naitza, Silvia; Zoledziewska, Magdalena; Mulas, Antonella; Pistis, Giorgio; Steri, Maristella; Danjou, Fabrice; Kwong, Alan; Ortega Del Vecchyo, Vicente Diego; Chiang, Charleston W K; Bragg-Gresham, Jennifer; Pitzalis, Maristella; Nagaraja, Ramaiah; Tarrier, Brendan; Brennan, Christine; Uzzau, Sergio; Fuchsberger, Christian; Atzeni, Rossano; Reinier, Frederic; Berutti, Riccardo; Huang, Jie; Timpson, Nicholas J; Toniolo, Daniela; Gasparini, Paolo; Malerba, Giovanni; Dedoussis, George; Zeggini, Eleftheria; Soranzo, Nicole; Jones, Chris; Lyons, Robert; Angius, Andrea; Kang, Hyun M; Novembre, John; Sanna, Serena; Schlessinger, David; Cucca, Francesco; Abecasis, Gonçalo R

    2015-11-01

    We report ∼17.6 million genetic variants from whole-genome sequencing of 2,120 Sardinians; 22% are absent from previous sequencing-based compilations and are enriched for predicted functional consequences. Furthermore, ∼76,000 variants common in our sample (frequency >5%) are rare elsewhere (<0.5% in the 1000 Genomes Project). We assessed the impact of these variants on circulating lipid levels and five inflammatory biomarkers. We observe 14 signals, including 2 major new loci, for lipid levels and 19 signals, including 2 new loci, for inflammatory markers. The new associations would have been missed in analyses based on 1000 Genomes Project data, underlining the advantages of large-scale sequencing in this founder population. PMID:26366554

  6. Environmental Chemicals in Urine and Blood: Improving Methods for Creatinine and Lipid Adjustment

    OpenAIRE

    O’Brien, Katie M.; Upson, Kristen; Cook, Nancy R.; Weinberg, Clarice R.

    2015-01-01

    Background Investigators measuring exposure biomarkers in urine typically adjust for creatinine to account for dilution-dependent sample variation in urine concentrations. Similarly, it is standard to adjust for serum lipids when measuring lipophilic chemicals in serum. However, there is controversy regarding the best approach, and existing methods may not effectively correct for measurement error. Objectives We compared adjustment methods, including novel approaches, using simulated case–con...

  7. Environmental Chemicals in Urine and Blood: Improving Methods for Creatinine and Lipid Adjustment

    OpenAIRE

    O’Brien, Katie M.; Upson, Kristen; Cook, Nancy R.; Weinberg, Clarice R.

    2015-01-01

    Background: Investigators measuring exposure biomarkers in urine typically adjust for creatinine to account for dilution-dependent sample variation in urine concentrations. Similarly, it is standard to adjust for serum lipids when measuring lipophilic chemicals in serum. However, there is controversy regarding the best approach, and existing methods may not effectively correct for measurement error. Objectives: We compared adjustment methods, including novel approaches, using simulated case–c...

  8. Effects of academic examination stress on eating behavior and blood lipid levels.

    Science.gov (United States)

    Pollard, T M; Steptoe, A; Canaan, L; Davies, G J; Wardle, J

    1995-01-01

    The influence of academic examination stress on eating behavior and lipid profiles and the moderating effect of dietary restraint, trait anxiety, and social support availability was assessed in university students. One hundred and seventy-nine students were divided into exam-stress groups (51 women, 64 men) and control groups (48 women, 16 men) and were assessed at baseline and then within 2 weeks of exams or an equivalent point for the control group. Perceived stress, emotional well-being, and fasting lipid profiles were measured, and dietary information was collected by interview. The exam-stress group reported significant increases in perceived stress and deterioration in emotional well-being at the exam sessions compared with baseline sessions. No general effects of exam stress on food intake were observed, and there was no interaction between stress and dietary restraint. However, students in the exam-stress group with high trait anxiety and low social support showed significant increases in total energy intake between baseline and exam sessions, whereas individuals with low trait anxiety and high social support showed a reduction in energy intake. Students with high trait anxiety and low social support showed increases between baseline and exam sessions in the amount of fat and saturated fat consumed. Women in the exam-stress group taking oral contraceptives showed a significant increase in total cholesterol between baseline and exam sessions. The results are discussed in relation to the effects of naturally occurring episodic stress on health behavior and on lipid profiles. PMID:16250770

  9. Changes in blood lipid in elderly population following morning exercise%健康老年人晨练活动后的血脂变化

    Institute of Scientific and Technical Information of China (English)

    陈静侬

    2002-01-01

    Objective To investigate mechanisms involving the effect of exercise on blood lipid in healthy elders.Method Healthy elders aged >60 years were included in study group,In control group,age,sex and body mathched persons were included.Blood lipid was evaluated for study group before and 3 months after morning exercise.Morning exercise consisted of walking with constant velocity(60~ 80 m/min) for 40~ 50 minutes,five times a week.Level of blood lipid in study group was compared with that of control group.Result TC,TG were significantly reduced and HE increased as compared with control group 3 months after exercise(P< 0.05).No changes occurred immediately and 20 hours after first exercise(P< 0.05).Conclusion Exercise can decrease TC,TG and raise HDL-C,which is beneficial to prevention of cerebrovascular and cardiovascular diseases in healthy elders.

  10. FADS1 FADS2 gene cluster, PUFA intake and blood lipids in children: results from the GINIplus and LISAplus studies.

    Directory of Open Access Journals (Sweden)

    Marie Standl

    Full Text Available BACKGROUND: Elevated cholesterol levels in children can be a risk factor for cardiovascular diseases in later life. In adults, it has been shown that blood lipid levels are strongly influenced by polymorphisms in the fatty acid desaturase (FADS gene cluster in addition to nutritional and other exogenous and endogenous determinants. Our aim was to investigate whether lipid levels are determined by the FADS genotype already in children and whether this association interacts with dietary intake of n-3 fatty acids. METHODS: The analysis was based on data of 2006 children from two German prospective birth cohort studies. Total cholesterol, HDL, LDL and triglycerides were measured at 10 years of age. Six single nucleotide polymorphisms (SNPs of the FADS gene cluster were genotyped. Dietary n-3 fatty acid intake was assessed by food frequency questionnaire. Linear regression modeling was used to assess the association between lipid levels, n-3 fatty acid intake and FADS genotype. RESULTS: Individuals carrying the homozygous minor allele had lower levels of total cholesterol [means ratio (MR ranging from 0.96 (p = 0.0093 to 0.98 (p = 0.2949, depending on SNPs] and LDL [MR between 0.94 (p = 0.0179 and 0.97 (p = 0.2963] compared to homozygous major allele carriers. Carriers of the heterozygous allele showed lower HDL levels [β between -0.04 (p = 0.0074 to -0.01 (p = 0.3318] and higher triglyceride levels [MR ranging from 1.06 (p = 0.0065 to 1.07 (p = 0.0028] compared to homozygous major allele carriers. A higher n-3 PUFA intake was associated with higher concentrations of total cholesterol, LDL, HDL and lower triglyceride levels, but these associations did not interact with the FADS1 FADS2 genotype. CONCLUSION: Total cholesterol, HDL, LDL and triglyceride concentrations may be influenced by the FADS1 FADS2 genotype already in 10 year old children. Genetically determined blood lipid levels during childhood might

  11. Effect of Pyramidal Training on Plasma Lipid Profile and Fibrinogen, and Blood Viscosity of Untrained Young Men

    Directory of Open Access Journals (Sweden)

    Abbass Ghanbari-Niaki

    2013-09-01

    Full Text Available The present study examined the effects of progressive exercise (pyramidal short-term program on plasma fibrinogen, lipid profile and blood viscosity in untrained young men. Changes and imbalances in homeostasis lead to cause of heart attacks. There is conflicting information about the effect of exercise on these factors. 19 young healthy untrained men were randomly assigned to the exercise group (n = 10 and controls (n = 9 groups. Exercise training group with increasing severity of heart maximum 25 to 100 percent began to run the practice (pyramid in 42-minute sessions, 3 times a week, for 4 weeks. There was no significant difference between the parameters of body composition, control, and training groups. Levels of total cholesterol and low density lipoprotein density and viscosity of the blood significantly decreased in the training group compared with the control group (p value, respectively is 0.001, 0.001 and 0.035. The changes in the concentration of fibrinogen, high-density lipoprotein and triglycerides in both groups are not significant in both groups (p value, respectively is 0.645, 0.993 and 0.421. The present results show that it is possible that progressive training (pyramidal short-term program changed the levels of cardiovascular risk factors by reducing blood viscosity.

  12. Hexadecenoic Fatty Acid Isomers in Human Blood Lipids and Their Relevance for the Interpretation of Lipidomic Profiles

    Science.gov (United States)

    Sansone, Anna; Tolika, Evanthia; Louka, Maria; Sunda, Valentina; Deplano, Simone; Melchiorre, Michele; Anagnostopoulos, Dimitrios; Chatgilialoglu, Chryssostomos; Formisano, Cesare; Di Micco, Rosa; Faraone Mennella, Maria Rosaria; Ferreri, Carla

    2016-01-01

    Monounsaturated fatty acids (MUFA) are emerging health biomarkers, and in particular the ratio between palmitoleic acid (9cis-16:1) and palmitic acid (16:0) affords the delta-9 desaturase index that is increased in obesity. Recently, other positional and geometrical MUFA isomers belonging to the hexadecenoic family (C16 MUFA) were found in circulating lipids, such as sapienic acid (6cis-16:1), palmitelaidic acid (9trans-16:1) and 6trans-16:1. In this work we report: i) the identification of sapienic acid as component of human erythrocyte membrane phospholipids with significant increase in morbidly obese patients (n = 50) compared with age-matched lean controls (n = 50); and ii) the first comparison of erythrocyte membrane phospholipids (PL) and plasma cholesteryl esters (CE) in morbidly obese patients highlighting that some of their fatty acid levels have opposite trends: increases of both palmitic and sapienic acids with the decrease of linoleic acid (9cis,12cis-18:2, omega-6) in red blood cell (RBC) membrane PL were reversed in plasma CE, whereas the increase of palmitoleic acid was similar in both lipid species. Consequentially, desaturase enzymatic indexes gave different results, depending on the lipid class used for the fatty acid content. The fatty acid profile of morbidly obese subjects also showed significant increases of stearic acid (C18:0) and C20 omega-6, as well as decreases of oleic acid (9cis-18:1) and docosahexaenoic acid (C22:6 omega-3) as compared with lean healthy controls. Trans monounsaturated and polyunsaturated fatty acids were also measured and found significantly increased in both lipid classes of morbidly obese subjects. These results highlight the C16 MUFA isomers as emerging metabolic marker provided that the assignment of the double bond position and geometry is correctly performed, thus identifying the corresponding lipidomic pathway. Since RBC membrane PL and plasma CE have different fatty acid trends, caution must also be used in the

  13. The Effects of Different Levels of Dietary Protein and L-Carnitine on Blood Sugar and Lipids of the New GIFT Strain of Juvenile Nile Tilapia (Oreochromis niloticus)

    OpenAIRE

    Gang Chen; Minghui Zhang; Jiandong Zhang; Hongbiao Dong; Hui Zhou; Baogui Tang; Jiansheng Huang; Gang Shi; Ling Jiang; Zhaohe Wu

    2009-01-01

    The new GIFT (Genetically Improved Farmed Tilapia) strain of Nile tilapia is a popular cultivated fish in Asia, but intensive aquaculture using nutritionally imbalanced feed has led to disorder of lipid metabolisms. An 8-week feeding experiment was conducted in order to assess the effects of different levels of L-carnitine (0, 200, 400, 600, and 800 mg/kg) and dietary protein (22, 25, and 28%) on blood sugar and blood lipid contents of the new juvenile GIFT strain of Nile tilapia. Results sho...

  14. Serum lipids and apolipoprotein B values, blood pressure and pulse rate in anorexia nervosa.

    Science.gov (United States)

    Sánchez-Muniz, F J; Marcos, A; Varela, P

    1991-01-01

    Some risk factors associated with coronary heart disease (CHD) were evaluated in patients with different types of anorexia nervosa (AN). Anthropometric parameters, serum cholesterol, triglycerides and apoprotein (apo) B values, blood pressure and pulse rate were tested in 29 young female patients and 16 controls. Cholesterol, triglycerides and apo B were higher at the acute period of the illness (AN1), whereas at the chronic period the values of these parameters tended to normalize. Triglycerides were higher in patients who binge ate (bulimarexia). Systolic blood pressure decreased in all types of AN, while diastolic blood pressure decreased only in AN1; pulse rate was not altered. According to cholesterol and apo B values, AN patients may be at risk of CHD if they remain at low body weight. PMID:1855497

  15. Placental lipoprotein lipase DNA methylation levels are associated with gestational diabetes mellitus and maternal and cord blood lipid profiles.

    Science.gov (United States)

    Houde, A A; St-Pierre, J; Hivert, M F; Baillargeon, J P; Perron, P; Gaudet, D; Brisson, D; Bouchard, L

    2014-04-01

    Placental lipoprotein lipase (LPL) is crucial for placental lipid transfer. Impaired LPL gene expression and activity were reported in pregnancies complicated by gestational diabetes mellitus (GDM) and intra-uterine growth restriction. We hypothesized that placental LPL DNA methylation is altered by maternal metabolic status and could contribute to fetal programming. The objective of this study was thus to assess whether placental LPL DNA methylation is associated with GDM and both maternal and newborn lipid profiles. Placenta biopsies were sampled at delivery from 126 women including 27 women with GDM diagnosed following a post 75 g-oral glucose tolerance test (OGTT) between weeks 24 and 28 of gestation. Placental LPL DNA methylation and expression levels were determined using bisulfite pyrosequencing and quantitative real-time PCR, respectively. DNA methylation levels within LPL proximal promoter region (CpG1) and intron 1 CpG island (CpGs 2 and 3) were lower in placenta of women with GDM. DNA methylation levels at LPL-CpG1 and CpG3 were also negatively correlated with maternal glucose (2-h post OGTT; r=-0.22; P=0.02) and HDL-cholesterol levels (third trimester of pregnancy; r=-0.20; p=0.03), respectively. Moreover, we report correlation between LPL-CpG2 DNA methylation and cord blood lipid profile. DNA methylation levels within intron 1 CpG island explained up to 26% (r⩽-0.51; Pmethylation dysregulation. Our results suggest that site-specific LPL epipolymorphisms in the placenta are possibly functional and could potentially be involved in determining the future metabolic health of the newborn. PMID:24847699

  16. Relationship and significance of serum leptin with blood insulin and lipid in 6-13 years old obese children

    International Nuclear Information System (INIS)

    To explore relationship and significance of Serum Leptin with BMI, Insulin, triglyceride (TG) and total cholesterol (TC) in obese children aged 6-13 years. Serum Leptin of school-age children 118 (64 male, 54 female; normal non-obese 56 and obese 62) were deter- mined and compared with BMI, Insulin, TG and TC. The results showed that: (1) Each index of obese children was remarkably higher than that of non-obese children (P0.05). (3) Leptin was poritinely corelation with BMI, insulin, TG and TC(P=0.001). Leptin level in serum may varied according to sex, BMI or blood lipid level. It is of great significance in prevention and treatment of obesity to use drug which may improve Leptin receptor effect. (authors)

  17. Effect of floorball training on blood lipids, body composition, muscle strength, and functional capacity of elderly men

    DEFF Research Database (Denmark)

    Petersen, Jacob Vorup; Pedersen, Mogens Theisen; Melcher, Pia Grethe Sandfeld;

    2016-01-01

    effect of floorball training on blood lipids, muscle strength, body composition, and functional capacity of men aged 65-76 years. Thirty-nine recreational active men were randomized into a floorball group (FG; n = 22) or petanque group (PG; n = 17), in which training was performed 1 h twice a week for 12...... functional capacity tests were better (P < 0.05) after compared to before the training period. No changes were observed in PG. In conclusion, 12 weeks of floorball training resulted in a number of favorable effects important for health and functional capacity, suggesting that floorball training can be used......Floorball training consists of intense repeated exercise and may offer a motivating and social stimulating team activity in elderly individuals. However, the effect of floorball training in elderly adults on physiological adaptations important for health is not known. Thus, this study examined the...

  18. Markers of antioxidant defence system and lipid peroxidation in peripheral blood of female patients with chronic idiopathic urticaria.

    Science.gov (United States)

    Kasperska-Zajac, Alicja; Brzoza, Zenon; Polaniak, Renata; Rogala, Barbara; Birkner, Ewa

    2007-03-01

    Oxidative stress is an important event in lesional skin of patients with chronic idiopathic urticaria (CIU). In the present study, we assessed blood oxidant/antioxidant status of patients suffering from CIU with positive response to autologous serum skin test (ASST) and with negative ASST, to improve our understanding of biological processes and the part of oxidative stress in this disease. Activities of manganese superoxide dismutase (MnSOD), copper-zinc superoxide dismutase (Cu/ZnSOD), glutathione peroxidase (GSH-PX), and catalase (CAT) as indices of enzymatic antioxidant capacity, as well as malondialdehyde (MDA) level as a maker of lipid peroxidation were measured in plasma and erythrocytes from 14 CIU female patients showing positive ASST, 31 CIU female patients with negative ASST and in 19 sex- and age-matched healthy subjects. The antioxidant enzyme activity in plasma and in erythrocytes did not differ significantly among the three groups. Also, the plasma and erythrocytes MDA levels were similar in the three groups. Based on our results, it seems that systemic activity of the enzymatic antioxidants (CuZn/SOD, MnSOD, GSH-Px, and CAT) as well as level of lipid peroxidation determined by MDA may not be increased in the course of immune-inflammatory processes associated with CIU. We also suggest that the systemic oxidant/antioxidant status of CIU patients, showing positive response to ASST, may not be different from that of CIU patients with negative ASST. PMID:17171548

  19. The Efficacy of Boswellia Serrata Gum Resin for Control of Lipid Profile and Blood Glucose in Diabetic Patients

    Science.gov (United States)

    Mehrzadi, Saeed; Tavakolifar, Bahreh; Huseini, Hasan Fallah; Mosavat, Seyed Hamdollah; Heydari, Mojtaba

    2016-01-01

    Background: Regarding preclinical evidence for antidiabetic effects of Boswellia serrata, we evaluated anti-hyperglycemic and lipid-lowering effects of Boswellia serrate gum resin in type 2 diabetic patients in a double-blind randomized placebo-control trial. Methods: Fifty-six diabetic patients were randomly allocated to two groups to receive 250 mg of the Boswellia serrate gum resin or placebo twice daily for 8 weeks, in addition to their routine antidiabetic treatments. Fasting blood sugar (FBS), glycosylated hemoglobin (HbA1c), insulin level, total cholesterol, low-density lipoprotein (LDL), high-density lipoprotein (HDL) and triglyceride of serum were measured before and after the intervention. Results: Although there was a considerable reduction after the intervention in FBS (P=0.04), HbA1c (P=0.02) and triglyceride (P=0.01) in the Boswellia serrate gum resin group, no significant difference was observed in all outcome measures between the two groups at the end of the study (FBS P=0.09, HbA1c P=0.20, total cholesterol P=0.31, LDL P=0.49, HDL P=0.10, triglyceride P=0.78 and insulin level P=0.86). Conclusion: The current study showed the 8 weeks complementary use of Boswellia serrate gum resin with a daily dose of 500 mg had no better glucose and lipid lowering effect than placebo in diabetic patients.

  20. Erythrocyte-bound apolipoprotein B in relation to atherosclerosis, serum lipids and ABO blood group.

    Directory of Open Access Journals (Sweden)

    Boudewijn Klop

    Full Text Available INTRODUCTION: Erythrocytes carry apolipoprotein B on their membrane, but the determining factors of erythrocyte-bound apolipoprotein B (ery-apoB are unknown. We aimed to explore the determinants of ery-apoB to gain more insight into potential mechanisms. METHODS: Subjects with and without CVD were included (N = 398. Ery-apoB was measured on fresh whole blood samples using flow cytometry. Subjects with ery-apoB levels ≤ 0.20 a.u. were considered deficient. Carotid intima media thickness (CIMT was determined as a measure of (subclinical atherosclerosis. RESULTS: Mean ery-apoB value was 23.2% lower in subjects with increased CIMT (0.80 ± 0.09 mm, N = 140 compared to subjects with a normal CIMT (0.57 ± 0.08 mm, N = 258 (P = 0.007, adjusted P<0.001. CIMT and ery-apoB were inversely correlated (Spearman's r: -0.116, P = 0.021. A total of 55 subjects (13.6% were considered ery-apoB deficient, which was associated with a medical history of CVD (OR: 1.86, 95% CI 1.04-3.33; adjusted OR: 1.55; 95% CI 0.85-2.82. Discontinuation of statins in 54 subjects did not influence ery-apoB values despite a 58.4% increase in serum apolipoprotein B. Subjects with blood group O had significantly higher ery-apoB values (1.56 ± 0.94 a.u. when compared to subjects with blood group A (0.89 ± 1.15 a.u, blood group B (0.73 ± 0.1.12 a.u. or blood group AB (0.69 ± 0.69 a.u. (P-ANOVA = 0.002. CONCLUSION: Absence or very low values of ery-apoB are associated with clinical and subclinical atherosclerosis. While serum apolipoprotein B is not associated with ery-apoB, the ABO blood group seems to be a significant determinant.

  1. [Effect of grape seed extracts on blood lipids in rabbits model with hyperlipidemia].

    Science.gov (United States)

    Yu, Hongxia; Zhao, Xiulan; Xu, Guifa; Wang, She-e

    2002-04-01

    The effect of grape seed extracts on serum lipids was observed in rabbits fed with high fat diet. The control group fed with normal stock diet. The diet of the four experimental groups was high in fat and cholesterol. The experimental control group fed with the high fat diet only, and the other three experimental groups fed the high fat diet added with grape seed extracts (5, 10 or 15 g/d) for three months. At the 0th, 6th and 12th week of the experiment, there was a lower serum TC, TG, LDL-C and a higher HDL-C in experimental groups in comparison with experimental control group. PMID:12561546

  2. Intravenous administration of brain-targeted stable nucleic acid lipid particles alleviates Machado-Joseph disease neurological phenotype.

    Science.gov (United States)

    Conceição, Mariana; Mendonça, Liliana; Nóbrega, Clévio; Gomes, Célia; Costa, Pedro; Hirai, Hirokazu; Moreira, João Nuno; Lima, Maria C; Manjunath, N; Pereira de Almeida, Luís

    2016-03-01

    Others and we showed that RNA interference holds great promise for the treatment of dominantly inherited neurodegenerative disorders such as Machado-Joseph disease (MJD), for which there is no available treatment. However, successful experiments involved intracranial administration of viral vectors and there is a need for a safer and less invasive procedure. In this work, we successfully generated stable nucleic acid lipid particles (SNALPs), incorporating a short peptide derived from rabies virus glycoprotein (RVG-9r) and encapsulating small interfering RNAs (siRNAs), which can target mutant ataxin-3. The developed formulation exhibited important features that make it adequate for systemic administration: high encapsulation efficiency of siRNAs, ability to protect the encapsulated siRNAs, appropriate and homogeneous particle size distribution. Following optimization of the formulation and in vitro validation of its efficacy to silence the MJD-causing protein - mutant ataxin-3 - in neuronal cells, in vivo experiments showed that intravenous administration of RVG-9r-targeted SNALPs efficiently silenced mutant ataxin-3 reducing neuropathology and motor behavior deficits in two mouse models of MJD. To our knowledge, this is the first report showing beneficial impact of a non-viral gene silencing strategy in MJD and the first time that a non-invasive systemic administration proved to be beneficial on a polyglutamine disorder. Our study opens new avenues towards MJD therapy that can also be applied to other neurodegenerative diseases linked to the production of pathogenic proteins. PMID:26757259

  3. 冠心病患者血压和血脂及血糖达标率分析%Analysis of the rates of achieving goal blood pressure, blood sugar and blood lipid in patients with coronary artery disease

    Institute of Scientific and Technical Information of China (English)

    李艳芳; 曹芳芳; 曹晓菁; 师树田; 刘飞; 王兆宏; 蒋志丽

    2011-01-01

    Objective To analyse the rates of achieving goal blood pressure, blood glucose and blood lipid in patients with CAD which was diagnosed by coronary arteriography and to explore the relationships between CAD and rates of reaching guidelines of the three risk factors, respectively. Methods The levels of blood pressure, blood sugar and blood lipid on admission were analyzed retrospectively in 2916 cases of CAD, and the level of blood pressure was analyzed when they were discharged. Results With 130/80 mm Hg as the goal blood pressure,the rate of achieving goal blood pressure was 28. 4% on admission,and was 87.5% when they were discharged. With 140/90 mm Hg as the goal blood pressure, the rate of achieving goal blood pressure was 65.8% on admission,and was 90.0% when they were discharged. With >1.04 mmol/L as the goal HDL-C,the rate of achieving goal HDL-C was 23.0% on admission. With <2.59 mmol/L and 2.07 mmol/L as the goal LDL-C in high risk patients and very high risk patients respectively, the rates of achieving goal LDL-C were 54. 3% and 17.4% respectively. With <4. 14 mmol/L and <3.11 mmol/L as the goal TC in high risk and very high risk patients,respectively,the rates of achieving goal TC were 49.5% and 5.8% respectively. With 1.70 mmol/L as the goal TG,the rate of achieving goal TG was 58.0%. There were 538 diabetic patients with CAD,accounting for 18.5 % ,their rates of achieving goal fasting blood glucose was 1.9 %, 1138 cases had diabetes and impaired fasting blood glucose,accounting for 39.0% in CAD. Conclusion The rates of achieving goal blood pressure,fasting blood glucose and blood lipids in patients with CAD were low on admission. The results show that enhancing the rates of achieving goal blood pressure, goal fasting blood glucose and goal blood lipids is very important for secondary prevention of CAD.%目的 分析经冠状动脉造影诊断为冠心病患者的血压、血脂和血糖达标率情况,评价其3个危

  4. Effects of Aqua Aerobics on Body Composition, Body Mass, Lipid Profile, and Blood Count in Middle-Aged Sedentary Women

    Directory of Open Access Journals (Sweden)

    Kantyka Joanna

    2015-03-01

    Full Text Available Purpose. The aim of the present investigations was to determine the effects of aqua aerobics on body weight and composition, lipid profile, and selected blood count parameters in middle-aged sedentary females. Methods. Twenty-one women were randomly assigned to an experimental group (age 56.20 ± 2.57 years, height 162.80 ± 4.76 cm, weight 74.03 ± 3.84 kg that participated in aqua aerobics classes three times a week for three months and a control group (mean age 56.44 ± 3.28 years, height 165.00 ± 3.91 cm, weight 70.01 ± 11.36 kg not involved in any kind of targeted exercise. The aqua aerobics classes were tailored to suit the age and abilities of the participants, with workout intensity controlled and maintained at approximately 128-137 bpm. Results. Significant differences between the experimental and control groups were found for body weight, total body water, fat-free mass, and skeletal muscle mass. A significant increase in post-intervention hemoglobin and erythrocyte counts was observed in the experimental group. Conclusions. Future studies should determine the intensity of physical activity with the most beneficial effect on blood variables in middle-aged and older individuals.

  5. Free-radical oxidation of lipids in erythrocyte membranes and endogenous alpha-tocopherol dynamics in blood plasma of acute leukemia patients

    Energy Technology Data Exchange (ETDEWEB)

    Karagezyan, K.G.; Bilyan, L.F.; Osipova, E.N.; Porosyan, A.S.

    1985-12-01

    Interaction between free-radical oxidation of lipids and dynamics of the endogenous alpha-tocopherol level in membrane erythrocytes was studied in healthy blood donors (14), acute leukemia patients before treatment (27) and persons in remission (11) after appropriate treatment by the commonly known VAMP, AVAMP and TsAMP procedures and, in some cases, by use of combinations of prednisolone and 6-mercaptopurine. Free-radical oxidation of lipids was determined by the malonic dialdehyde yield in ascorbate- and NADPH-independent oxidation systems. The studies showed an inversely proportional dependence between reduction of the alpha-tocopherol level in the acute leukemia patients blood plasma and the intensity of free radical oxidation of lipids in the erythrocyte membranes. The combined therapy recommended in the study inhibited the peroxide formation process in the erythrocyte membranes significantly and promoted restoration of the endogenous alpha-tocopherol level which facilitated a favorable course of acute leukemia and promoted possible remission.

  6. Effects of a milk product, fermented by Lactobacillus acidophilus and with fructo-oligosaccharides added, on blood lipids in male volunteers

    NARCIS (Netherlands)

    Schaafsma, G.; Meuling, W.J.A.; Dokkum, W. van; Bouley, C.

    1998-01-01

    Objective: To investigate in adult male volunteers the effect of a new fermented milk product, fermented by Lactobacillus acidophilus and with fructo-oligosaccharides added, on blood lipids. Design: Randomized placebo-controlled double-blind two-way cross over trial with two treatment periods of thr

  7. Somatic-cell selection is a major determinant of the blood-cell phenotype in heterozygotes for glucose-6-phosphate dehydrogenase mutations causing severe enzyme deficiency

    Energy Technology Data Exchange (ETDEWEB)

    Filosa, S.; Giacometti, N.; Wangwei, C.; Martini, G. [Istituto Internazionale di Genetica e Biofisica, Naples (Italy)] [and others

    1996-10-01

    X-chromosome inactivation in mammals is regarded as an essentially random process, but the resulting somatic-cell mosaicism creates the opportunity for cell selection. In most people with red-blood-cell glucose-6-phosphate dehydrogenase (G6PD) deficiency, the enzyme-deficient phenotype is only moderately expressed in nucleated cells. However, in a small subset of hemizygous males who suffer from chronic nonspherocytic hemolytic anemia, the underlying mutations (designated class I) cause more-severe G6PD deficiency, and this might provide an opportunity for selection in heterozygous females during development. In order to test this possibility we have analyzed four heterozygotes for class I G6PD mutations: two with G6PD Portici (1178G{r_arrow}A) and two with G6PD Bari (1187C{r_arrow}T). We found that in fractionated blood cell types (including erythroid, myeloid, and lymphoid cell lineages) there was a significant excess of G6PD-normal cells. The significant concordance that we have observed in the degree of imbalance in the different blood-cell lineages indicates that a selective mechanism is likely to operate at the level of pluripotent blood stem cells. Thus, it appears that severe G6PD deficiency affects adversely the proliferation or the survival of nucleated blood cells and that this phenotypic characteristic is critical during hematopoiesis. 65 refs., 6 figs., 3 tabs.

  8. A new PAMPA model using an in-house brain lipid extract for screening the blood-brain barrier permeability of drug candidates.

    Science.gov (United States)

    Bicker, Joana; Alves, Gilberto; Fortuna, Ana; Soares-da-Silva, Patrício; Falcão, Amílcar

    2016-03-30

    The determination of the permeability of drug candidates across the blood-brain barrier (BBB) is a fundamental step during drug discovery programs. The parallel artificial membrane permeability assay (PAMPA) is a high throughput screening tool applied to evaluate the passive permeability and adapted to predict BBB penetration. Herein, a new PAMPA model was developed using an in-house brain lipid extract capable of discriminating BBB permeable from non-permeable compounds. The apparent permeability (Papp) of 18 reference molecules and 10 test compounds was assessed and compared with phosphatidylcholine and commercial porcine polar brain lipid (PBL). The physicochemical selectivity of the in-house brain lipid extract was demonstrated by correlating Papp values with physicochemical properties and its predictive capacity estimated by establishing in vitro-in vivo correlations. The strong correlations achieved between 2% (w/v) in-house lipid extract and PBL for reference (r(2)=0.77) and test compounds (r(2)=0.94) support an equivalent discriminatory capacity and validate the presented model. Moreover, PAMPA studies performed with PBL and in-house lipid extract exhibited a higher correlation with the in vivo parameter logBB (r(2)=0.76 and r(2)=0.72, respectively) than phosphatidylcholine (r(2)=0.51). Overall, the applied lipid extraction process was reproducible, economical and provided lipid extracts that can be used to reliably assess BBB permeation. PMID:26836708

  9. Effects of IGFS on blood lipid metabolism in experimental hyperlipidemia rats

    International Nuclear Information System (INIS)

    Objective: To observe the effects of injection of ginseng fruit saponins (IGFS) on total cholesterol (TC), lipoprotein cholesterol metabolism and antioxidative activity in experimental hyperlipidemia rats. Methods: The TC, lipoprotein cholesterol and lipidperoxidation (LPO) contents, prostacycline (PGI2) and thromboxane (TXA2) levels, superoxidedismutase (SOD) activity and blood viscosity were measured respectively in hyperlipidemia rats which had been given IGFS 10, 20 and 40 mg·kg-1·d-'1 ip, respectively, for fifteen days. In addition, fat accumulation in liver was observed. Results: The triglyceride (TG), TC, low density lipoprotein cholesterol (LDL-c) in serum, TXA2 in plasma, LPO in serum and liver and blood viscosity were decreased significantly, and PGI2 in plasma and SOD in serum and liver were significantly increased after administration with IGFS (20 and 40 mg·kg-1·d-1) in experimental hyperlipidemia rats. Moreover, IGFS decreased ratios of TC/HDL-c and LDL-c/HDL-c, increased the ratio of PGI2/TXA2 and inhibit fat accumulation in liver. The content of high density lipoprotein cholesterol (HDL-c) in serum were significantly increased after administration IGFS (40 mg·kg-1·-1) in experimental hyperlipidemia rats. Conclusions: IGFS can inhibit arterioscleros by improving cholesterol and lipoprotein cholesterol metabolism, suppressing LPO and increasing antioxidation. (authors)

  10. Higher frequency of secretor phenotype in O blood group – its benefits in prevention and/or treatment of some diseases

    Directory of Open Access Journals (Sweden)

    Mohamad Salih Jaff

    2010-11-01

    Full Text Available Mohamad Salih JaffPathology Department, Hawler Medical University (Formerly Salahaddin University, Erbil, Kurdistan Region, IraqAbstract: ABO blood groups and secretor status are important in clinical and forensic medicine and in relation to some diseases. There are geographic and racial differences in their frequencies, but the frequency of secretor status in different ABO blood group systems has not been determined yet. Therefore, the aim of this study was mainly to determine this point. Blood and saliva from 762 randomly selected apparently healthy adult individuals (480 men and 282 women were examined to determine their ABO and Rhesus blood groups by standard conventional methods, and their secretor status by using Lewis blood grouping and/or hemagglutination inhibition test of saliva. Results showed that 76.1% of the study population were ABH blood group antigens secretors and 23.9% were nonsecretors. The frequencies of secretor status in different ABO blood groups were 70.1% in group A, 67.8% in group B, 67.9% in group AB, and 88.3% in group O. In conclusion, blood group O individuals have significantly higher frequency of secretor status than non-O blood group individuals. This finding would be beneficial to them, protecting them, at least partially, from certain malignancies or allowing them to have less aggressive disease, and this finding might be useful in enhancing further studies and research in this direction.Keywords: blood group O, ABO blood groups, secretor phenotype, frequency, malignancies, prevention and/or treatment

  11. 高龄老年人脉压差、血脂、血糖关系分析%Analysis of the Correlation between Pulse Pressure,Blood Lipid,Blood Sugar in the Elderly People

    Institute of Scientific and Technical Information of China (English)

    丁翊; 刘芳

    2013-01-01

    Objective:Removed the age factor, to probe into the correlation between blood pressure,blood lipid,blood sugar and arteriosclerosis in the elderly people. Methods:Analyzed the correlation between Pulse Pressure,Mean Arterial blood pressure and Diabetes mellitus,Hypertension in 279 examination of the elderly. analyzed the changes of blood glucose,blood lipid at the different levels of Pulse Pressure. In order to know the correlation between Pulse Pressure,Mean Arterial blood pressure and blood glucose,blood lipid, blood pressure. Results:There was a correlation between arteriosclerosis and arterial compliance in the elderly people and hyperglycemia,the disorder of lipid metabolism and high systolic blood pressure. Conclusions:Strengthened the blood glucose management and the treatment of dyslipidemia in patients with diabetes mellitus, strengthened the control of blood pressure in patients with essential hypertension, these measures had a positive effect on prevention and treatment of senile arteriosclerosis.%目的:探讨老年动脉硬化除去年龄因素以外与血压、血糖、血脂的关系。方法:体检的老年人279例,分析脉压、平均动脉压与糖尿病、高血压病的关系及不同脉压水平下血糖、血脂的变化,进一步了解脉压、平均动脉压与血糖、血脂、血压之间的关系。结果:高龄老年人动脉硬化、血管顺应性减低与血糖增高、血脂代谢紊乱、收缩压增高有关。结论:加强糖尿病患者的血糖管理及血脂紊乱的治疗、加强高血压病患者血压控制对老年动脉硬化的防治有积极作用。

  12. Effect of whey protein on blood lipid profiles: a meta-analysis of randomized controlled trials.

    Science.gov (United States)

    Zhang, J-W; Tong, X; Wan, Z; Wang, Y; Qin, L-Q; Szeto, I M Y

    2016-08-01

    Previous studies have suggested that whey supplementation may have beneficial effects on lipid profiles, although results were inconsistent. A literature search was performed in March 2015 for randomized controlled trials observing the effects of whey protein and its derivatives on circulating levels of triacylglycerol (TG), total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C) and high-density lipoprotein cholesterol (HDL-C). A meta-analysis was subsequently conducted. The meta-analysis results of 13 trials showed that whey supplementation significantly reduced the circulating TG level by 0.11 mmol/l (95% CI: -0.21, 0 mmol/l), whereas the whey protein had no effects on circulating TC (-0.11 mmol/l, 95% CI: -0.27, 0.05 mmol/l), LDL-C (-0.08 mmol/l, 95% CI: -0.23, 0.07 mmol/l) and HDL-C (0.01 mmol/l, 95% CI: -0.04, 0.05 mmol/l). Subgroup analysis showed that significant TG reduction disappeared in participants with low body mass index, low supplemental whey dose or under exercise training/energy restriction during the trial. No evidence of heterogeneity across studies and publication bias was observed. In conclusion, our findings demonstrated that the effects of whey protein supplementation were modest, with an overall lowering effect on TG but no effect on TC, LDL-C and HDL-C. PMID:27026427

  13. THE CHARACTERISTICS OF BLOOD LIPIDS OF MENOPAUSAL WOMEN WITH THE COMPENSATED HYPOTHYROIDISM WHICH RESULTS FROM AUTOIMMUNE THYROIDITIS

    Directory of Open Access Journals (Sweden)

    Yu. A. Malyshenko

    2015-12-01

    Full Text Available Purpose: to study the lipid profile, including of non-high-density lipoproteins cholesterol (non-HDL-C, in postmenopausal women with compensated hypothyroidism. The causes of hypothyroidism included Hashimoto thyroiditis.45 women with hypothyroidism participated in the study (mean ± sd, age (57.4 ± 7.7 years, disease duration (8.0 ± 6.4 years, the duration of postmenopause (6.4 ± 3.5 years. The mean dose of L-T4 (84.3 ± 28.5 μg/d. The control group – 85 women (mean ± sd, age (58.4 ± 5.4 years no abnormalities of the thyroid gland, as well as other chronic diseases, which could have an impact on lipid metabolism.The main and control group were matched for age. Average BMI basic group than in controls: (31.6 ± 3.4 and (28.7 ± 4.6 kg/m2 respectively (p = 0.001. Obtained statistical differences in terms of TSH in the study and control groups: 2.15 and 1,22 mU/L (p = 0.001. Upon reaching euthyrosis against the background of hormone replacement therapy with thyroid hormones do not reach the target total cholesterol (TC, low density lipoproteins cholesterol (LDL-C: 5.81 ± 1.14, 3.67 ± 1.06 respectively.We obtain lower high-density lipoproteins cholesterol (HDL-C levels in women with drug euthyroidism compared with the control group. Do not get the difference in the values non-HDL-C in the groups studied. In postmenopausal women with compensated hypothyroidism is defined negative correlation ATTPO with HDL-C. Mean values of blood TC, non-HDL-C, LDL-C levels in the groups exceed the optimal ones.

  14. Effect of Vit C Supplement on Fasting Blood Glucose and Plasma Lipid Level in Type II Diabetic Patients

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    Mehri Delvarianzadeh

    2008-01-01

    Full Text Available Introduction: Diabetic people are more at risk of diseases such as vascular, kidneys and eye diseases, compared with normal people. Since Vit C competitively replaces glucose in most chemical reactions; so inhibit on non enzymatic glycosylation such as hemoglobin and lipoproteins, it seems that Vit C can be effective to prevent diabetic complications .In this study effect of Vit C supplements on blood sugar and fats level in type II diabetic patients was investigated. Methods: This study was conducted as a clinical trial using paired random sampling on 136 type II diabetic patients. At first, the patients weights, age, type of diet were recorded .Then their fasting blood glucose (FBS, glycosylated hemoglobin (HbAIC, Cholesterol, triglyceride, HDL and LDL were measured. After that the patients were divided in two randomized groups. The first group (control was subject of administration of Vit C supplement (1250 mg, 5 times a day, for a period of 3 months and the second group received placebo. Data were analyzed by paired t- test and SPSS Software. Results: In this study most of patients were women (62.2% of control group and 64.7% of placebo group. The mean age between the control group and placebo group were reported 51.2 ± 6.8 and 50.37± 9.71 years respectively. At the end of study , after 3 months of administration of Vit C supplement to control group, the mean concentration of FBS, LDL triglyceride Cholesterol and glycosylated hemoglobin has been reduced significantly with respect to the placebo group and the mean value of HDL has been increased as well. Conclusion: The finding shows that Vit C supplements might reduce the amount of glucose, lipids and glycosylated hemoglobin in patients with type 2 diabetes. Therefore, its consumption in diabetic patients can be recommended and as a result patient complications will be significantly reduced.

  15. Effects of agave nectar versus sucrose on weight gain, adiposity, blood glucose, insulin, and lipid responses in mice.

    Science.gov (United States)

    Hooshmand, Shirin; Holloway, Brittany; Nemoseck, Tricia; Cole, Sarah; Petrisko, Yumi; Hong, Mee Young; Kern, Mark

    2014-09-01

    Agave nectar is a fructose-rich liquid sweetener derived from a plant, and is often promoted as a low glycemic alternative to refined sugar. However, little scientific research has been conducted in animals or humans to determine its metabolic and/or health effects. The aim of this study was to explore the influence of agave nectar versus sucrose on weight gain, adiposity, fasting plasma blood glucose, insulin, and lipid levels. Eighteen (n=18) male ICR mice (33.8±1.6 g) were divided into two groups (n=6 for agave nectar and n=12 for sucrose) and provided free access to one of two diets of equal energy densities differing only in a portion of the carbohydrate provided. Diets contained 20% carbohydrate (by weight of total diet) from either raw agave nectar or sucrose. Epididymal fat pads were excised, and blood was collected after 34 days. Weight gain (4.3±2.2 vs. 8.4±3.4 g), fat pad weights (0.95±0.54 vs. 1.75±0.66 g), plasma glucose (77.8±12.2 vs. 111.0±27.9 mg/dL), and insulin (0.61±0.29 vs. 1.46±0.81 ng/mL) were significantly lower (P≤.05) for agave nectar-fed mice compared to sucrose-fed mice respectively. No statistically significant differences in total cholesterol or triglycerides were detected. These results suggest that in comparison to sucrose, agave nectar may have a positive influence on weight gain and glucose control. However, more research with a larger sample of animals and/or with human subjects is warranted. PMID:25011004

  16. The Effects of Different Levels of Dietary Protein and L-Carnitine on Blood Sugar and Lipids of the New GIFT Strain of Juvenile Nile Tilapia (Oreochromis niloticus

    Directory of Open Access Journals (Sweden)

    Gang Chen

    2009-01-01

    Full Text Available The new GIFT (Genetically Improved Farmed Tilapia strain of Nile tilapia is a popular cultivated fish in Asia, but intensive aquaculture using nutritionally imbalanced feed has led to disorder of lipid metabolisms. An 8-week feeding experiment was conducted in order to assess the effects of different levels of L-carnitine (0, 200, 400, 600, and 800 mg/kg and dietary protein (22, 25, and 28% on blood sugar and blood lipid contents of the new juvenile GIFT strain of Nile tilapia. Results showed that dietary protein and L-carnitine had significant influences on glucose (GLU, high-density lipoprotein–cholesterol (HDL-C, total cholesterol (TC, triglyceride (TG, and low-density lipoprotein–cholesterol (LDL-C in the blood serum. The contents of GLU and HDL-C increased with the increases in dietary protein and L-carnitine levels, while the contents of TC, LDL-C, and TG decreased with the increases in dietary protein and L-carnitine levels. The interactive effect of both dietary protein and L-carnitine was most significant on GLU (p = 0.0001, followed by TG (p = 0.001, TC (p = 0.005, HDL-C (p = 0.056, and LDL-C (p = 0.109. These results suggested that high levels of dietary protein and L-carnitine supplementation reduce blood lipids and the burden of the fish liver.

  17. Correlation between lipid peroxidation process in placental tissue and blood of pregnant women living at the territories with low level of radionuclide contamination

    International Nuclear Information System (INIS)

    The purpose of the investigation was analysis of lipid peroxidation processes in placental tissue and blood of 89 pregnant women living on the territory of the Gomel Region with low level of radioactive contamination (1 5 Ci/km2) in comparison with these parameters at 65 pregnant women living on territory which was relatively free from radionuclides. The groups of women didn't differ from each other by the age and period of gestation. Vein blood and placental tissue were used to determine malonic dialdehyde content and the level of vitamins A, E and C. Activation of lipid peroxidation and impairment of antioxidant system were established in blood of pregnant women living in regions with radionuclide contamination. Placental tissue of these women was less affected by the action of low dose irradiation. Nevertheless tendency of increasing of lipid peroxidation and decreasing of vitamin C metabolism were estimated. Disbalance of oxidant and antioxidant processes in blood of pregnant women is early marker of such breach in foetoplacental system. Support of antioxidant status of pregnant women is necessary for prophylactic of peroxidation syndrome and complications of gestation

  18. The Lipid Moiety of Haemozoin (Malaria Pigment and P. falciparum Parasitised Red Blood Cells Bind Synthetic and Native Endothelin-1

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    Nicoletta Basilico

    2010-01-01

    Full Text Available Endothelin1 (ET-1 is a 21-amino acid peptide produced by the vascular endothelium under hypoxia, that acts locally as regulator of vascular tone and inflammation. The role of ET-1 in Plasmodium falciparum malaria is unknown, although tissue hypoxia is frequent as a result of the cytoadherence of parasitized red blood cell (pRBC to the microvasculature. Here, we show that both synthetic and endothelial-derived ET-1 are removed by parasitized RBC (D10 and W2 strains, chloroquine sensitive, and resistant, resp. and native haemozoin (HZ, malaria pigment, but not by normal RBC, delipidized HZ, or synthetic beta-haematin (BH. The effect is dose dependent, selective for ET-1, but not for its precursor, big ET-1, and not due to the proteolysis of ET-1. The results indicate that ET-1 binds to the lipids moiety of HZ and membranes of infected RBCs. These findings may help understanding the consequences of parasite sequestration in severe malaria.

  19. Clinical trial of Cecropia obtusifolia and Marrubium vulgare leaf extracts on blood glucose and serum lipids in type 2 diabetics.

    Science.gov (United States)

    Herrera-Arellano, A; Aguilar-Santamaría, L; García-Hernández, B; Nicasio-Torres, P; Tortoriello, J

    2004-11-01

    Cecropia obtusifolia and Marrubium vulgare have been widely used in Mexican traditional medicine for the control of type 2 diabetes. In order to evaluate the clinical effect produced by the aqueous extract from these species on type 2 non-controlled diabetes mellitus, a total of 43 outpatients were included. Based on the European NIDDM (policy group) criteria, only patients with poor response to the conventional treatment were selected. All patients maintained their medical treatment and also received a prepared infusion of the dry leaves of the plant treatment for 21 days. In a double-blind manner, the patients were randomly grouped as follows: 22 patients were treated with C. obtusifolia and 21 with M. vulgare. The fasting blood glucose values were reduced by 15.25% on patients treated with C. obtusifolia, while cholesterol and triglycerides were decreased by 14.62% and 42.0%, respectively (ANOVA pvulgare, the plasma glucose level was reduced by 0.64% and cholesterol and triglycerides by 4.16% and 5.78%, respectively. When the results were compared between groups, significant differences in glucose and cholesterol diminution were found. The obtained results showed that the infusion prepared with the leaves of C. obtusifolia (containing 2.99+/-0.14mg of chlorogenic acid/g of dried plant) produced beneficial effects on carbohydrate and lipid metabolisms when it was administered as an adjunct on patients with type 2 diabetes with poor response to conventional medical treatment. PMID:15636168

  20. Study of changes in serum lipid profile and blood sugar level by perindopril and telmisartan during treatment of systemic hypertension

    Directory of Open Access Journals (Sweden)

    Manish Kumar

    2014-06-01

    Results: With perindopril initial means of TC, HDL, LDL, TGs, FBS, and PPBS in Groups A and B were 190.32, 49.76, 117.96, 165.04, 84.56, 122.60, and 188.80, 51.64, 118.52, 159.12, 93.92, 133.60, respectively. After 24 weeks, these values were 190.84, 50.68, 118.60, 163.84, 83.48, 120.20, and 190.96, 52.04, 118.28, 157.56, 93.96, 133.68, respectively (p > 0.05. With telmisartan, initial means of TC, HDL, LDL, TG, FBS, and PPBS in both groups were 188.08, 49.76, 118.84, 167.20, 83.72, 120.68, and 188.08, 46.88, 121.96, 167.84, 91.44, 131.72, respectively. After 24 weeks, these values in both groups were 189.36, 49.80, 120.04, 165.96, 82.60, 118.36 and 186.12, 45.28, 121.08, 167.72, 92.76, 129.56 respectively (p > 0.05. Conclusions: It concluded that both perindopril and telmisartan had not any significant adverse effects on plasma lipid profile and blood sugar level in both groups. [Int J Basic Clin Pharmacol 2014; 3(3.000: 454-459

  1. Correlation between "ABO" blood group phenotypes and periodontal disease: Prevalence in south Kanara district, Karnataka state, India

    Directory of Open Access Journals (Sweden)

    Gurpur Prakash Pai

    2012-01-01

    Full Text Available Background: The correlation between certain systemic diseases and ABO blood group is a well-documented fact. The association between periodontal disease and ABO blood group is not studied in relation to a specific geographic location. Here is a study conducted on a group of patients belonging to South Kanara district of Karnataka state. Materials and Methods: A total of 750 subjects aged between 30and 38 years belonging to South Kanara district were selected on random basis. The study subjects were segregated into healthy/mild gingivitis, moderate/severe gingivitis, and periodontitis group, based on Loe and Silness index and clinical attachment loss as criteria. The study group was further categorized and graded using Ramfjord′s periodontal disease index. Blood samples were collected to identify ABO blood group. Results: Prevalence of blood group O was more in South Kanara district, followed by blood groups B and A, and the least prevalent was AB. The percentage distribution of subjects with blood groups O and AB was more in healthy/mild gingivitis group (group I and moderate/severe gingivitis group (group II, while subjects with blood groups B and A were more in periodontitis group III. There was increased prevalence of subjects with blood groups O and AB with healthy periodontium, while subjects with blood groups B and A showed inclination toward diseased periodontium. Conclusion: There is a correlation existing between periodontal disease and ABO blood group in this geographic location. This association can be due to various blood group antigens acting as receptors for infectious agents associated with periodontal disease. This broad correlation between periodontal disease and ABO blood group also points toward susceptibility ofthe subjects with certain blood groups to periodontal disease.

  2. Establishment of a microplate assay for flow cytometric assessment and it is use for the evaluation of age-related phenotypic changes in canine whole blood leukocytes.

    Science.gov (United States)

    Reis, Alexandre B; Carneiro, Cláudia M; Carvalho, Maria das Graças; Teixeira-Carvalho, Andréa; Giunchetti, Rodolfo C; Mayrink, Wilson; Genaro, Odair; Corrêa-Oliveira, Rodrigo; Martins-Filho, Olindo A

    2005-02-10

    The effectiveness of flow cytometric assays for canine use is still requiring standardization. Despite several studies using purified mononuclear cells, no methodology or reference ranges are available for immunophenotyping of whole blood leukocytes (WBL). Fresh and pre-fixed WBL were used to identify cell-subsets, (Thy-1(+)/CD5(+)/CD4(+)/CD8(+)/CD21(+) and CD14(+)) and measure MHC-II, CD45RA/CD45RB expression. We described here an efficient method for fast quantification of canine-WBL, using pre-fix in a microplate assay, which allows long-term sample storage prior to phenotyping. Decreased percentage of CD5(+)-T-cells within the lymphocyte-gate and increased percentage of CD21(+)-B-cells were observed in young animals, which led to higher T/B cell ratios in middle-aged dogs. Lower numerical counts of Thy-1(+), CD4(+), CD8(+) and CD21(+) lymphocyte were observed when compared to young animals. In addition, we identified an age-related decline of MHC-II/CD45RA expression by lymphocytes. We proposed an improved method for phenotyping of canine peripheral blood mononuclear cells (PBMC) that has significant use for researchers and veterinary clinicians. The hematological changes of senescence previously identified on PBMC could be adequately reproduced on features identified by whole blood. Furthermore, this study supplies normal range references as baseline standards for clinical purposes, besides specific immunological parameters to monitor canine aging process. PMID:15621304

  3. Dynamics of change of lipid and monoamine metabolisms and the blood coagulation system during experimental atherosclerosis caused by restriction of movement

    Science.gov (United States)

    Gvishiani, G. S.; Kobakhidze, N. G.

    1980-01-01

    Shifts in lipid, catecholamine, and blood coagulation systems following various periods (1, 2, 3, and 4 months) of experimentally induced atherosclerosis were studied. The same indices were studied in the tissues of the myocardium, liver, and brain stem-reticular formation after decapitation of the animals at the end of the experiment. Periodic motion restriction caused an increase in blood beta-lipoproteins in the rabbits at the beginning of the experiment. An increase in general cholesterol content and a decrease in the lecithincholesterol index were established at the end of the experiment. Myocardial beta-lipoprotein and brain stem reticular formation general cholesterol contents were elevated; catecholamine content was increased at the end of the experiment. In the initial months, free adrenaline basically increased, while in later months blood adrenaline decreased and blood noradrenaline increased.

  4. Effects of supplementation with acai (Euterpe oleracea Mart.) berry-based juice blend on the blood antioxidant defence capacity and lipid profile in junior hurdlers. A pilot study

    OpenAIRE

    Sadowska-Krępa, E; Kłapcińska, B; Podgórski, T; Szade, B; Tyl, K; Hadzik, A

    2015-01-01

    The purpose of this pilot study was to examine whether regular consumption of an acai berry-based juice blend would affect sprint performance and improve blood antioxidant status and lipid profile in junior athletes. Seven junior hurdlers (17.5±1.2 years) taking part in a pre-season conditioning camp were supplemented once a day, for six weeks, with 100 ml of the juice blend. At the start and the end of the camp the athletes performed a 300-m sprint running test on an outdoor track. Blood sam...

  5. Cordyceps sinensis biomass produced by submerged fermentation in high-fat diet feed rats normalizes the blood lipid and the low testosterone induced by diet

    OpenAIRE

    Aikawa, Júlia; Bonatto, Sandro José Ribeiro; Freire dos Santos, Leandro; Habu, Sascha; Prado, Karin Braun.; Rubel, Rosália; Soccol, Carlos Ricardo; Soccol, Vanete Thomaz; De Torres, Maria Fernanda; Yamaguchi, Adriana Aya; Zanatta, Ana Lucia

    2013-01-01

    This study investigated the effect of Cordyceps sinensis biomass supplementation obtained from submerged fermentation on blood lipid and low testosterone induced by high-fat diet (HFD). The experiments were carried out using a long-term intake of HFD and HFD plus Simvastatin or C. sinensis (4 months). Our results show that plasma cholesterol, triglycerides and LDL were decreased by Cordyceps sinensis biomass supplementation (CSBS). A longterm intake of HFD caused a significant liver damage wh...

  6. Effects of the extract from roasted chicory (Cichorium intybus L.) root containing inulin-type fructans on blood glucose, lipid metabolism, and fecal properties

    OpenAIRE

    Nishimura, Mie; Ohkawara, Tatsuya; Kanayama, Toshiyuki; Kitagawa, Kazuya; Nishimura, Hiroyuki; Nishihira, Jun

    2015-01-01

    The extract from roasted chicory (Cichorium intybus L.; 菊苣 jú jù) root (chicory root extract), which contains inulin-type fructans, has favorable effects including antihyperglycemic and antidyslipidemic effects and the improvement of bowel movement. In this study, we examined the effects of chicory root extract on blood glucose, lipid metabolism, and fecal properties in 47 healthy adult participants in a randomized, double-blind, placebo-controlled study. The participants were divided into a ...

  7. Effect of Orange (Citrus sinensis) Peel Oil on Lipid Peroxidation, Catalase activity and Hepatic Biomarker levels in Blood Plasma of Normo Rats

    OpenAIRE

    Erukainure, Ochuko L.

    2012-01-01

    Dietary antioxidants are considered beneficial because of their potential protective role against oxidative stress, which is involved in the pathogenesis of multiple diseases such as cancer and coronary heart disease. The effect of feeding orange peel oil on lipid peroxidation, catalase and hepatic biomarkers in blood plasma of normo rats was investigated. Beside mouse chow, four diets were designed to contain 50% of energy as carbohydrate, 35% as fat, and 15% as protein, and one that was lip...

  8. Effects of seaweed supplementation on blood glucose concentration, lipid profile, and antioxidant enzyme activities in patients with type 2 diabetes mellitus

    OpenAIRE

    Kim, Min Sun; Kim, Jung Yun; Choi, Woong Hwan; Lee, Sang Sun

    2008-01-01

    The present study was carried out to evaluate the physiological effects of seaweed supplementation on blood glucose levels, lipid profile, and antioxidant enzyme activities in subjects with type 2 diabetes mellitus. Subjects were randomized into either a control group or a seaweed supplementation group. Pills with equal parts of dry powdered sea tangle and sea mustard were provided to the seaweed supplementation group three times a day for 4 weeks. Total daily consumption of seaweed was 48 g....

  9. Effects of soybean supplementation on blood glucose, plasma lipid levels, and erythrocyte antioxidant enzyme activity in type 2 diabetes mellitus patients

    OpenAIRE

    Chang, Ji Ho; Kim, Min Sun; Kim, Tae Wha; Lee, Sang Sun

    2008-01-01

    The purpose of this study was to investigate the effect of soybean on blood glucose and lipid concentrations, and antioxidant enzyme activity in type 2 diabetes mellitus (DM) patients. We divided patients into two groups and fed them, respectively, a basal diet (control group) and a basal diet with 69 g/d of soybean (soybean group) for 4 weeks. Pills with roasted soybean powder were provided to the soybean supplementation group three times a day. Macronutrients intake except dietary fiber was...

  10. The influence of a ten-week Nordic walking training-rehabilitation program on the level of lipids in blood in overweight and obese postmenopausal women.

    Science.gov (United States)

    Hagner-Derengowska, Magdalena; Kałużny, Krystian; Hagner, Wojciech; Kochański, Bartosz; Plaskiewicz, Anna; Borkowska, Alina; Bronisz, Agata; Budzyński, Jacek

    2015-10-01

    [Purpose] The aim of this study was to evaluate the effect of a ten-week Nordic Walking (NW) rehabilitation program on chosen anthropometric parameters and the level of basic lipids in overweight and obese postmenopausal women's blood. [Subjects and Methods] The subjects were 32 women aged 50-68 (average: 59.7 ± 5.9 years). The study was carried out following a non-randomized model and entailed NW rehabilitation 5 times a week, which lasted for 10 weeks, as well as a low-calorie 1,500 kcal diet. The therapeutic results of the study were measured through changes in anthropometric and biochemical parameters. The results were subjected to a statistical analysis. [Results] After 10 weeks of NW rehabilitation it was observed that participants lost weight and their body mass index dropped. Additionally, whereas levels of total cholesterol, LDL and triglycerides dropped, and the level of HDL increased. [Conclusion] Rehabilitation carried out according to the NW model resulted in statistically significant changes in basic lipids in blood which, considerably increased the percentage of persons who achieved the recommended level of blood lipids. Obese persons were characterised by a smaller rehabilitation weight loss. More intense workouts and cooperation with a dietician are required. PMID:26644639

  11. From Phenotype to Genotype: Enter Genomics and Transformation of Primary Health Care around the World

    OpenAIRE

    Slavkin, H. C.

    2014-01-01

    The progress in phenotype descriptions, measurements, and analyses has been remarkable in the last 50 years. Biomarkers (proteins, carbohydrates, lipids, hormones, various RNAs and cDNAs, microarrays) have been discovered and correlated with diseases and disorders, as well as physiological responses to disease, injury, stress, within blood, urine, and saliva. Three-dimensional digital imaging advanced how we “see” and utilize phenotypes toward diagnosis, treatment, and prognosis. In each exam...

  12. Clinical Symptoms in Fibromyalgia Are Better Associated to Lipid Peroxidation Levels in Blood Mononuclear Cells Rather than in Plasma

    Science.gov (United States)

    Cano-García, Francisco J.; De Miguel, Manuel; Carrión, Angel M.; Navas, Plácido; Sánchez Alcázar, José A.

    2011-01-01

    Background We examined lipid peroxidation (LPO) in blood mononuclear cells (BMCs) and plasma, as a marker of oxidative damage, and its association to clinical symptoms in Fibromyalgia (FM) patients. Methods We conducted a case–control and correlational study comparing 65 patients and 45 healthy controls. Clinical parameters were evaluated using the Fibromyalgia Impact Questionnaire (FIQ), visual analogues scales (VAS), and the Beck Depression Inventory (BDI). Oxidative stress was determined by measuring LPO in BMCs and plasma. Results We found increased LPO levels in BMCs and plasma from FM patients as compared to normal control (P<0.001). A significant correlation between LPO in BMCs and clinical parameters was observed (r = 0.584, P<0.001 for VAS; r = 0.823, P<0.001 for FIQ total score; and r = 0.875, P<0.01 for depression in the BDI). We also found a positive correlation between LPO in plasma and clinical symptoms (r = 0.452, P<0.001 for VAS; r = 0.578, P<0.001 for FIQ total score; and r = 0.579, P<0.001 for depression in the BDI). Partial correlation analysis controlling for age and BMI, and sex, showed that both LPO in cells and plasma were independently associated to clinical symptoms. However, LPO in cells, but not LPO in plasma, was independently associated to clinical symptoms when controlling for depression (BDI scores). Discussion The results of this study suggest a role for oxidative stress in the pathophysiology of fibromyalgia and that LPO in BMCs rather than LPO in plasma is better associated to clinical symptoms in FM. PMID:22046409

  13. FRUCTOOLIGOSACCHARIDE (FOS SUPPLEMENTATION IN TYPE 2 DIABETIC ADULTS IMPROVES SYSTOLIC BLOOD PRESSURE, SERUM LIPID, AND GUT MICROBIOTA

    Directory of Open Access Journals (Sweden)

    Mahendra A

    2013-12-01

    Full Text Available Fructooligosaccharide (FOS is a prebiotic, becoming apparent for its therapeutic role for diseased conditions like CHD, hypertension, diabetes and obesity. CHD and hypertension are one of the most prevalent NCD’s encountered in diabetic adults. Present study aims to assess the effect of FOS supplementation in type 2 diabetic adults on their lipemic, biophysical parameters and gut microflora parameters. A cross-sectional study was designed with 65 adult type 2 diabetics enrolled from Health clinic of The M.S. University of Baroda, Gujarat. All the subjects were randomly divided into two groups control and experimental. The experimental group was given 10 g of fructooligosaccharide and compared with the controls for lipemic parameters, hypertension and fecal counts in terms of lactic acid bacteria, bifidobacteria and enteric pathogen. Eight week supplementation of FOS resulted in an appreciable reduction in serum TC, TG and LDL levels by 10%, 4.9% and 7.8% respectively. A significant reduction (p<0.05 in systolic blood pressure was also observed as a result of FOS supplementation in the experimental group. A decline was seen in TC/HDL, LDL/HDL and non-HDL by 10.4%, 7.6% and 6.6% respectively (p<0.05, p<0.001. Gut microbiota values exhibited a significant increment in fecal log10 counts of Lactic acid bacteria and bifidobacteria by 9.3% and 10.9% respectively (p<0.001 while a significant reduction by 4.8% (p<0.001 was observed for Enteric pathogen. These outcomes revealed an efficacy of FOS in reducing serum lipid parameters, systolic BP and improving the gut microbiota in type 2 diabetic adults

  14. 血尿酸浓度与慢性心力衰竭患者的血糖血脂血压相关性研究%Analysis on the relationships between serum uric acid concentration and blood -sugar, blood -lipid, and blood- pressure in patients with chronic heart failure

    Institute of Scientific and Technical Information of China (English)

    宋健雄

    2011-01-01

    Objective To explore the relationships between serum uric acid(SUA) concentration and blood - sugar, blood lipid, and blood - pressure in patients with chronic heart failure. Methods Eighty -four patients suffering from chronic heart failure were used as the observation group and another 84 healthy people with normal heart function as the control group. The SUA, blood -pressure, fasting blood- glucose, blood- lipid including cholesterol, three acids glyceride, high -density lipo- protein, and low- density lipo- protein were determined in two groups. Results Compared with the control group, the patients with chronic heart failure have higher incidence in high SUA, high choles-terol, high blood - pressure, and abnormality in blood - sugar and blood - lipid. ( P < 0.05 ).Conclusions High SUA is relative to blood - sugar, blood - lipid, and blood - pressure in patients with chronic heart failure.%目的 探讨血尿酸浓度与慢性心力衰竭患者血糖、血脂、血压的关系.方法 选择84例慢性心力衰竭患者为观察组,另选84例心功能正常者为对照组,测定血尿酸、血压、空腹血糖、血脂,包括血浆总胆固醇、甘油三酯、高密度脂蛋白、低密度脂蛋白.结果 慢性心力衰竭组中高尿酸、高血压、血糖和血脂异常的发生率较高,与对照组比较差异有统计学意义(P<0.05).结论 高尿酸血症与慢性心力衰竭患者的血糖、血脂、血压水平密切相关.

  15. Versatile lipid profiling by liquid chromatography–high resolution mass spectrometry using all ion fragmentation and polarity switching. Preliminary application for serum samples phenotyping related to canine mammary cancer

    International Nuclear Information System (INIS)

    Graphical abstract: -- Highlights: •Lipidomics, high resolution mass spectrometry, polarity switching, serum, canine mammary cancer. -- Abstract: Lipids represent an extended class of substances characterized by such high variety and complexity that makes their unified analyses by liquid chromatography coupled to either high resolution or tandem mass spectrometry (LC–HRMS or LC–MS/MS) a real challenge. In the present study, a new versatile methodology associating ultra high performance liquid chromatography coupled to high resolution tandem mass spectrometry (UHPLC–HRMS/MS) have been developed for a comprehensive analysis of lipids. The use of polarity switching and “all ion fragmentation” (AIF) have been two action levels particularly exploited to finally permit the detection and identification of a multi-class and multi-analyte extended range of lipids in a single run. For identification purposes, both higher energy collision dissociation (HCD) and in-source CID (collision induced dissociation) fragmentation were evaluated in order to obtain information about the precursor and product ions in the same spectra. This approach provides both class-specific and lipid-specific fragments, enhancing lipid identification. Finally, the developed method was applied for differential phenotyping of serum samples collected from pet dogs developing spontaneous malignant mammary tumors and health controls. A biological signature associated with the presence of cancer was then successfully revealed from this lipidome analysis, which required to be further investigated and confirmed at larger scale

  16. Versatile lipid profiling by liquid chromatography–high resolution mass spectrometry using all ion fragmentation and polarity switching. Preliminary application for serum samples phenotyping related to canine mammary cancer

    Energy Technology Data Exchange (ETDEWEB)

    Gallart-Ayala, H., E-mail: laberca@oniris-nantes.fr [LUNAM, Ecole Nationale Vétérinaire, Agroalimentaire et de l’Alimentation Nantes Atlantique (Oniris), USC 1329 INRA Laboratoire d’Etude des résidus et Contaminants dans les Aliments (LABERCA), Site de la Chantrerie – CS50707, 44307 Nantes cedex 3 (France); Courant, F.; Severe, S.; Antignac, J.-P. [LUNAM, Ecole Nationale Vétérinaire, Agroalimentaire et de l’Alimentation Nantes Atlantique (Oniris), USC 1329 INRA Laboratoire d’Etude des résidus et Contaminants dans les Aliments (LABERCA), Site de la Chantrerie – CS50707, 44307 Nantes cedex 3 (France); Morio, F.; Abadie, J. [LUNAM, Ecole Nationale Vétérinaire, Agroalimentaire et de l’Alimentation Nantes Atlantique (Oniris), Cancers Animaux, Modèles pour la Recherche en Oncologie Comparée (AMaROC), Site de la Chantrerie–CS50707, 44307 Nantes cedex 3 (France); Le Bizec, B. [LUNAM, Ecole Nationale Vétérinaire, Agroalimentaire et de l’Alimentation Nantes Atlantique (Oniris), USC 1329 INRA Laboratoire d’Etude des résidus et Contaminants dans les Aliments (LABERCA), Site de la Chantrerie – CS50707, 44307 Nantes cedex 3 (France)

    2013-09-24

    Graphical abstract: -- Highlights: •Lipidomics, high resolution mass spectrometry, polarity switching, serum, canine mammary cancer. -- Abstract: Lipids represent an extended class of substances characterized by such high variety and complexity that makes their unified analyses by liquid chromatography coupled to either high resolution or tandem mass spectrometry (LC–HRMS or LC–MS/MS) a real challenge. In the present study, a new versatile methodology associating ultra high performance liquid chromatography coupled to high resolution tandem mass spectrometry (UHPLC–HRMS/MS) have been developed for a comprehensive analysis of lipids. The use of polarity switching and “all ion fragmentation” (AIF) have been two action levels particularly exploited to finally permit the detection and identification of a multi-class and multi-analyte extended range of lipids in a single run. For identification purposes, both higher energy collision dissociation (HCD) and in-source CID (collision induced dissociation) fragmentation were evaluated in order to obtain information about the precursor and product ions in the same spectra. This approach provides both class-specific and lipid-specific fragments, enhancing lipid identification. Finally, the developed method was applied for differential phenotyping of serum samples collected from pet dogs developing spontaneous malignant mammary tumors and health controls. A biological signature associated with the presence of cancer was then successfully revealed from this lipidome analysis, which required to be further investigated and confirmed at larger scale.

  17. Defining Molecular Phenotypes of Mesenchymal and hematopoietic Stem Cells derived from Peripheral blood of Acute Lymphocytic Leukemia patients for regenerative stem cell therapy

    Science.gov (United States)

    Potdar, PD; Subedi, RP

    2011-01-01

    Acute Lymphocytic Leukemia (ALL) is a clonal myeloid disorder affecting all age groups, characterized by accumulation of immature blast cells in bone marrow and in peripheral blood. Autologous Bone Marrow Transplantation is a present treatment for cure of ALL patients, which is very expensive, invasive process and may have possibility of transplantation of malignant stem cells to patients. In the present study, we hypothesized to isolate large number of normal Mesenchymal & Hematopoietic stem cells from peripheral blood of ALL patients, which will be further characterized for their normal phenotypes by using specific molecular stem cell markers. This is the first study, which defines the existing phenotypes of isolated MSCs and HSCs from peripheral blood of ALL patients. We have established three cell lines in which two were Mesenchymal stem cells designated as MSCALL and MSCnsALL and one was suspension cell line designated as HSCALL. The HSCALL cell line was developed from the lymphocyte like cells secreted by MSCALL cells. Our study also showed that MSCALL from peripheral blood of ALL patient secreted hematopoietic stem cells in vitro culture. We have characterized all three-cell lines by 14 specific stem cell molecular markers. It was found that both MSC cell lines expressed CD105, CD13, and CD73 with mixed expression of CD34 and CD45 at early passage whereas, HSCALL cell line expressed prominent feature of hematopoietic stem cells such as CD34 and CD45 with mild expression of CD105 and CD13. All three-cell lines expressed LIF, OCT4, NANOG, SOX2, IL6, and DAPK. These cells mildly expressed COX2 and did not express BCR-ABL. Overall it was shown that isolated MSCs and HSCs can be use as a model system to study the mechanism of leukemia at stem cell level and their use in stem cell regeneration therapy for Acute Lymphocytic Leukemia. PMID:24693170

  18. Defining Molecular Phenotypes of Mesenchymal and hematopoietic Stem Cells derived from Peripheral blood of Acute Lymphocytic Leukemia patients for regenerative stem cell therapy

    Directory of Open Access Journals (Sweden)

    Pravin D. Potdar

    2011-01-01

    Full Text Available Acute Lymphocytic Leukemia (ALL is a clonal myeloid disorder affecting all age groups, characterized by accumulation of immature blast cells in bone marrow and in peripheral blood. Autologous Bone Marrow Transplantation is a present treatment for cure of ALL patients, which is very expensive, invasive process and may have possibility of transplantation of malignant stem cells to patients. In the present study, we hypothesized to isolate large number of normal Mesenchymal & Hematopoietic stem cells from peripheral blood of ALL patients, which will be further characterized for their normal phenotypes by using specific molecular stem cell markers. This is the first study, which defines the existing phenotypes of isolated MSCs and HSCs from peripheral blood of ALL patients. We have established three cell lines in which two were Mesenchymal stem cells designated as MSCALL and MSCnsALL and one was suspension cell line designated as HSCALL. The HSCALL cell line was developed from the lymphocyte like cells secreted by MSCALL cells. Our study also showed that MSCALL from peripheral blood of ALL patient secreted hematopoietic stem cells in vitro culture. We have characterized all three-cell lines by 14 specific stem cell molecular markers. It was found that both MSC cell lines expressed CD105, CD13, and CD73 with mixed expression of CD34 and CD45 at early passage whereas, HSCALL cell line expressed prominent feature of hematopoietic stem cells such as CD34 and CD45 with mild expression of CD105 and CD13. All three-cell lines expressed LIF, OCT4, NANOG, SOX2, IL6, and DAPK. These cells mildly expressed COX2 and did not express BCR-ABL. Overall it was shown that isolated MSCs and HSCs can be use as a model system to study the mechanism of leukemia at stem cell level and their use in stem cell regeneration therapy for Acute Lymphocytic Leukemia.

  19. Whole Grain Rye Intake, Reflected by a Biomarker, Is Associated with Favorable Blood Lipid Outcomes in Subjects with the Metabolic Syndrome – A Randomized Study

    Science.gov (United States)

    Magnusdottir, Ola Kally; Landberg, Rikard; Gunnarsdottir, Ingibjorg; Cloetens, Lieselotte; Åkesson, Björn; Rosqvist, Fredrik; Schwab, Ursula; Herzig, Karl-Heinz; Hukkanen, Janne; Savolainen, Markku J.; Brader, Lea; Hermansen, Kjeld; Kolehmainen, Marjukka; Poutanen, Kaisa; Uusitupa, Matti; Risérus, Ulf; Thorsdottir, Inga

    2014-01-01

    Background and Aim Few studies have explored the possible plasma cholesterol lowering effects of rye consumption. The aim of this secondary analysis in the SYSDIET study was to investigate the association between plasma alkylresorcinols (AR), a biomarker for whole grain wheat and rye intake, and blood lipid concentrations in a population with metabolic syndrome. Furthermore, we analyzed the associations between the AR C17∶0/C21∶0 ratio, a suggested marker of the relative intake of whole grain/bran rye, and blood lipid concentrations. Methods Participants were 30–65 years of age, with body mass index (BMI) 27–40 kg/m2 and had metabolic syndrome. Individuals were recruited through six centers in the Nordic countries and randomized either to a healthy Nordic diet (ND, n = 93), rich in whole grain rye and wheat, as well as berries, fruits and vegetables, rapeseed oil, three fish meals per week and low-fat dairy products, or a control diet (n = 65) for 18/24 weeks. Associations between total plasma AR concentration and C17∶0/C21∶0 homologue ratio and blood lipids were investigated in pooled (ND + control group) regression analyses at 18/24 weeks adjusted for baseline value for the dependent variable, age, BMI and statin use. Results When adjusted for confounders, total plasma AR at 18/24 weeks was not significantly associated with blood lipids but the AR ratio C17∶0/C21∶0 was inversely associated with LDL cholesterol concentrations (B (95% CI): −0.41 (−0.80 to −0.02)), log LDL/HDL cholesterol ratio (−0.20 (−0.37 to −0.03)), log non-HDL cholesterol (−0.20 (−0.37 to −0.03)), log apolipoprotein B (−0.12 (−0.24 to 0.00)) and log triglyceride concentrations (−0.35 (−0.59 to −0.12)). Discussion Increased proportion of whole grain rye, reflected by a biomarker, in the diet is associated with favorable blood lipid outcomes, a relationship that should be further investigated. Trial Registration ClinicalTrials.gov NCT00992641

  20. The influence of radiographic phenotype and smoking status on peripheral blood biomarker patterns in chronic obstructive pulmonary disease.

    Directory of Open Access Journals (Sweden)

    Jessica M Bon

    Full Text Available BACKGROUND: Chronic obstructive pulmonary disease (COPD is characterized by both airway remodeling and parenchymal destruction. The identification of unique biomarker patterns associated with airway dominant versus parenchymal dominant patterns would support the existence of unique phenotypes representing independent biologic processes. A cross-sectional study was performed to examine the association of serum biomarkers with radiographic airway and parenchymal phenotypes of COPD. METHODOLOGY/PRINCIPAL FINDINGS: Serum from 234 subjects enrolled in a CT screening cohort was analyzed for 33 cytokines and growth factors using a multiplex protein array. The association of serum markers with forced expiratory volume in one second percent predicted (FEV1% and quantitative CT measurements of airway thickening and emphysema was assessed with and without stratification for current smoking status. Significant associations were found with several serum inflammatory proteins and measurements of FEV1%, airway thickening, and parenchymal emphysema independent of smoking status. The association of select analytes with airway thickening and emphysema was independent of FEV1%. Furthermore, the relationship between other inflammatory markers and measurements of physiologic obstruction or airway thickening was dependent on current smoking status. CONCLUSIONS/SIGNIFICANCE: Airway and parenchymal phenotypes of COPD are associated with unique systemic serum biomarker profiles. Serum biomarker patterns may provide a more precise classification of the COPD syndrome, provide insights into disease pathogenesis and identify targets for novel patient-specific biological therapies.

  1. n-3 PUFA status in school children is associated with beneficial lipid profile, reduced physical activity and increased blood pressure in boys

    DEFF Research Database (Denmark)

    Damsgaard, Camilla T.; Stark, Ken D.; Hjorth, Mads F.;

    2013-01-01

    relationship between fasting whole-blood EPA or DHA (w/w% of the total fatty acids, FA%) and markers of the MetS (anthropometry, blood pressure, plasma lipids and glucose homeostasis) cross-sectionally in seventy-three 8–11-year-old Danish children from the OPUS School Meal Pilot Study (OPUS is an acronym of......:TAG increased with whole-blood EPA (β>0·25, P<0·05), and HDL increased 0·35 (sem 0·13) mmol/l per FA% EPA increase (β = 0·30, P= 0·008). Unexpectedly, DHA was positively associated with mean arterial pressure in boys (6·3 (sem 1·7) mmHg/FA% DHA increase, β = 0·62, P= 0·001) and reduced physical activity in both...

  2. Influence of diet with kale on lipid peroxides and malondialdehyde levels in blood serum of laboratory rats over intoxication with paraquat

    Directory of Open Access Journals (Sweden)

    Elżbieta Sikora

    2013-03-01

    Full Text Available Background. Organism’s lipid peroxidation is one of the most often examined and known physiological process evoked by free radicals. It concerns oxidation reaction of unsaturated fatty acid and/or other lipids leading to lipid oxidation products (LOP, which as a result of further changes generate among others the malondialdehyde molecules. The aim of the work was an estimation if raw or cooked kale addition to rat’s diet infl uences antioxidant defense effi ciency in their organisms in comparison to rats fed with standard AIN-93G diet. Material and methods. The experiment was conducted with 36 Wistar strain, male rats over 21 days. The rats were divided into 3 groups (each 12 stuck which were fed with: standard diet AIN-93G (2 groups, AIN-93G diet with 10% addition of raw kale (2 groups, and AIN-93G with 10% addition of cooked lyophilised kale. The total content of polyphenols (FC method and antioxidant activity (ABTS+• were previously determined in raw and then in cooked kale. On the 20th day of experiment, half of rats (6 stuck of each kind of the diet were injected intraperitoneally by the solution of paraquat (PQ in physiological salt to evoke the oxidative stress. The next day animals were stunned and blood from their hearts was sampled. In the obtained serum, the levels of lipid oxidation products (LOP and malondialdehyde (MDA were assessed. Results. It was observed that in blood serum of rats fed with modifi ed diet with raw and cooked lyophilised kale addition the lipid oxides level was lower in comparison to control group fed with standard diet (p < 0.05. It was found that intoxication with paraquat caused growth of MDA and LOP levels in blood serum of all rats in comparison to not intoxicated groups but that growth was the lowest in group fed diet with cooked kale addition. Conclusion. Diet with kale, both raw and cooked, effi ciently inhibited the lipid peroxidation process in rats’ organisms, ongoing during natural metabolism

  3. Beneficial effects of simulated gastro-intestinal digests of fried egg and its fractions on blood pressure, plasma lipids and oxidative stress in spontaneously hypertensive rats.

    Directory of Open Access Journals (Sweden)

    Forough Jahandideh

    Full Text Available We have previously characterized several antihypertensive peptides in simulated digests of cooked eggs and showed blood pressure lowering property of fried whole egg digest. However, the long-term effects of this hydrolysate and its fractions on blood pressure are not known. Therefore, the objectives of the study were to determine the effects of long term administration of fried whole egg hydrolysate and its fractions (i.e. egg white and egg yolk on regulation of blood pressure and associated factors in cardiovascular disease such as plasma lipid profile and tissue oxidative stress.We used spontaneously hypertensive rats (SHR, an animal model of essential hypertension. Hydrolysates of fried egg and its fractions were prepared by simulated gastro-intestinal digestion with pepsin and pancreatin. 16-17 week old male SHRs were orally administered fried whole egg hydrolysate, non-hydrolyzed fried whole egg, egg white hydrolysate or egg yolk hydrolysates (either defatted, or not daily for 18 days. Blood pressure (BP and heart rate were monitored by telemetry. Animals were sacrificed at the end of the treatment for vascular function studies and evaluating plasma lipid profile and tissue oxidative stress. BP was reduced by feeding fried whole egg hydrolysate but not by the non-hydrolyzed product suggesting a critical role for in vitro digestion in releasing anti-hypertensive peptides. Egg white hydrolysate and defatted egg yolk hydrolysate (but not egg yolk hydrolysate also had similar effects. Reduction in BP was accompanied by the restoration of nitric oxide (NO dependent vasorelaxation and reduction of plasma angiotensin II. Fried whole egg hydrolysate also reduced plasma levels of triglyceride although it was increased by the non-hydrolyzed sample. Additionally the hydrolyzed preparations attenuated tissue oxidative stress.Our results demonstrate that fried egg hydrolysates exert anti-hypertensive effects, improve plasma lipid profile and attenuate

  4. Relation between blood lipid levels and diet and characterization of food choices in children with familial hypercholesterolemia

    OpenAIRE

    2012-01-01

    Background and aims: Familial hypercholesterolemia (FH) is a genetic disorder characterized by elevated levels of low density lipoprotein (LDL) cholesterol and increased risk of premature cardiovascular disease (CVD). The treatment of FH involves lipid-lowering drug therapy and dietary counseling, the latter being the primary treatment in children. The observed lipid lowering- and CVD preventing effects of certain dietary patterns in the general population support the inclusion of dietary cou...

  5. Superoxide dismutase and lipid hydroperoxides in blood and endometrial tissue of patients with benign, hyperplastic and malignant endometrium

    Directory of Open Access Journals (Sweden)

    Snežana Pejić

    2008-09-01

    Full Text Available Epidemiological and experimental data point to involvement of oxygen derived radicals in the pathogenesis of gynecological disorders, as well as in cancer development. The objective of the present study was to examine changes in activities and levels of copper/zinc superoxide dismutase (CuZnSOD and lipid hydroperoxides (LOOH in blood and endometrial tissue of patients diagnosed with uterine myoma, endometrial polypus, hyperplasia simplex, hyperplasia complex and adenocarcinoma endometrii. The results of our study have shown decreased SOD activities and unchanged SOD protein level in blood of all examined patients in comparison to healthy subjects. Decrease of both SOD activity and level was found in endometrium of patients with hyperplasia simplex, hyperplasia complex and adenocarcinoma in comparison to women with polypus or myoma. LOOH level was elevated in both tissues of patients with hyperplasiaor adenocarcinoma in comparison to healthy subjects or patients with benign diagnosis. Our findings suggest that the decrease in SOD activity and level, as well as the increase in LOOH level, in patients with gynecological disorders, render these patients more susceptible to oxidative damage caused by reactive oxygen species (ROS. An imbalance in ROS formation and SOD level may be important in the pathogenesis and/or perpetuation of tissue damage in gynecological patients. Since evidence suggests that SOD may be a therapy target for cancer treatment, our findings provide a basis for further research and options for clinical applications.Resultados epidemiológicos e experimentais apontam para o envolvimento dos radicais derivados do oxigênio na patogênese das moléstias ginecológicas, assim como no desenvolvimento do câncer. O objetivo do presente estudo foi o de examinar as alterações nas atividades e níveis de Cu/Zn superóxido dismutase (CuZnSOD e hidroperóxidos lipídicos (LOOHno sangue e tecido endometrial de pacientes diagnosticados com

  6. Perfil lipídico e estado nutricional de adolescentes Blood lipid levels and nutritional status of adolescents

    Directory of Open Access Journals (Sweden)

    Danielle Franklin de Carvalho

    2007-12-01

    Full Text Available OBJETIVOS: Avaliar a associação entre obesidade e dislipidemias em adolescentes do ensino público e privado de Campina Grande-PB, Brasil. MÉTODOS: Estudo transversal com 180 adolescentes de 14 a 17 anos matriculados no ensino público e privado de Campina Grande-PB. O estado nutricional foi classificado segundo os percentis do Índice de Massa Corporal para sexo e idade. Foi realizada coleta sangüínea para avaliação do perfil lipídico (colesterol total, frações HDL-colesterol, LDL-colesterol e triglicerídeos. Considerou-se dislipidemia a existência de alteração em pelo menos um dos exames. As análises estatísticas foram realizadas a partir do Epi Info 3.3 e Stata 7.0. RESULTADOS: A prevalência de sobrepeso foi de 14,4% enquanto que 83,9% dos estudantes eram eutróficos e 1,7% apresentavam baixo peso. Não foram encontradas diferenças estatisticamente significantes para o estado nutricional quando estratificado por sexo e tipo de escola. Todas as taxas bioquímicas investigadas mostraram algum nível de alteração. Chamou à atenção a prevalência de dislipidemia, observada em 66,7% dos estudantes, e a alteração do HDL-colesterol, verificada em 56,7% destes. Registrou-se associação estatisticamente significante (pOBJECTIVE: To evaluate the association between obesity and dyslipidemia in adolescents from public and private schools in Campina Grande, PB, Brazil. METHODS: Cross-sectional study of 180 adolescents, aged between 14 and 17 years, enrolled in the public and private school system of Campina Grande-PB. The nutritional status was classified according to the percentiles of Body Mass Index for gender and age. A blood sample was taken to evaluate the lipid profile (total cholesterol, HDL-cholesterol, LDL-cholesterol, and triglyceride levels. Dyslipidemia was considered when at least one of the blood parameters exceeded reference limits. Statistical analysis was performed with Epi Info 3.3 and Stata 7.0. RESULTS: The

  7. 银屑病患者血细胞参数及血脂分析的临床意义%Clinical significance of analysis of blood cell parameters and blood lipids in patients with psoriasis

    Institute of Scientific and Technical Information of China (English)

    吉晓云

    2014-01-01

    Objective To investigate the clinical significance of the determination of related blood cell parameters and blood lipids for psoriasis. Methods Eleven kinds of blood cell parameters, cholesterol and blood fat were detected between fifty-two cases of patients with psoriasis in my hospital and one hundred cases of normal controls at the same time. Results Compared with the control group, MCV, RDW, MPV, PDW, CH, TG of psoriasis group and the psoriasis group in activity were significantly higher. Conclusion Psoriasis and cardiovascular disease have the same basis of pathophysiology. Abnormal blood cell parameters and blood lipids are therefore the important reasons why psoriasis is easy with cardiovascular disease.%目的:探讨血细胞参数及血脂有关项目测定对银屑病的临床意义。方法我院银屑病患者52例和正常对照100例同时检测11项血细胞参数及胆固醇和血脂。结果银屑病及其进行期MCV、RDW、MPV、PDW、CH、TG均显著高于对照人群。结论银屑病与心血管疾病的病理及生理基础有相同之处,所以银屑病患者易发心血管疾病与血细胞参数及血脂的变化有关。

  8. Modulatory effect of pineapple peel extract on lipid peroxidation, catalase activity and hepatic biomarker levels in blood plasma of alcohol-induced oxidative stressed rats

    Institute of Scientific and Technical Information of China (English)

    Okafor OY; Erukainure OL; Ajiboye JA; Adejobi RO; Owolabi FO; Kosoko SB

    2011-01-01

    Objective: To investigate the ability of the methanolic extract of pineapple peel to modulate alcohol-induced lipid peroxidation, changes in catalase activities and hepatic biochemical marker levels in blood plasma. Methods: Oxidative stress was induced by oral administration of ethanol (20% w/v) at a dosage of 5 mL/kg bw in rats. After 28 days of treatment, the rats were fasted overnight and sacrificed by cervical dislocation. Blood was collected with a 2 mL syringe by cardiac puncture and was centrifuged at 3000 rpm for 10 min. The plasma was analyzed to evaluate malondialdehyde (MDA), catalase activity, aspartate aminotransferase (AST), alkaline phosphatase (ALP) and alanine aminotransferase (ALT) concentrations. Results: Administration of alcohol caused a drastic increase (87.74%) in MDA level compared with the control. Pineapple peel extract significantly reduced the MDA level by 60.16% at 2.5 mL/kg bw. Rats fed alcohol only had the highest catalase activity, treatment with pineapple peel extract at 2.5 mL/kg bw however, reduced the activity. Increased AST, ALP and ALT activities were observed in rats fed alcohol only respectively, treatment with pineapple peel extract drastically reduced their activities. Conclusions: The positive modulation of lipid peroxidation, catalase activities as well as hepatic biomarker levels of blood plasma by the methanolic extract of pineapple peels under alcohol-induced oxidative stress is an indication of its protective ability in the management of alcohol-induced toxicity.

  9. Weight gain is associated with improved glycaemic control but with adverse changes in plasma lipids and blood pressure isn Type 1 diabetes.

    LENUS (Irish Health Repository)

    Ferriss, J B

    2012-02-03

    AIMS: To assess the effects of weight gain on metabolic control, plasma lipids and blood pressure in patients with Type 1 diabetes. METHODS: Patients in the EURODIAB Prospective Complications Study (n = 3250) were examined at baseline and 1800 (55%) were re-examined a mean of 7.3 years later. Patients had Type 1 diabetes, defined as a diagnosis made before age 36 years and with a need for continuous insulin therapy within a year of diagnosis. Patients were aged 15-60 years at baseline and were stratified for age, sex and duration of diabetes. RESULTS: The change in HbA(1c) from baseline to follow-up examination was significantly more favourable in those who gained 5 kg or more during follow-up (\\'marked weight gain\\') than in patients who gained less or no weight or lost weight (\\'less or no weight gain\\'). In those with marked weight gain, there was a significantly greater rise in plasma triglycerides and total cholesterol and significantly less favourable changes in low-density lipoprotein and high-density lipoprotein cholesterol compared with those with less or no weight gain, with or without adjustment for HbA(1c). Systolic and diastolic blood pressure also rose significantly more in the group with marked weight gain. CONCLUSION: Weight gain in patients with Type 1 diabetes has adverse effects on plasma lipids and blood pressure, despite a small improvement in glycaemic control.

  10. Effects of supplementation with acai (Euterpe oleracea Mart.) berry-based juice blend on the blood antioxidant defence capacity and lipid profile in junior hurdlers. A pilot study.

    Science.gov (United States)

    Sadowska-Krępa, E; Kłapcińska, B; Podgórski, T; Szade, B; Tyl, K; Hadzik, A

    2015-06-01

    The purpose of this pilot study was to examine whether regular consumption of an acai berry-based juice blend would affect sprint performance and improve blood antioxidant status and lipid profile in junior athletes. Seven junior hurdlers (17.5±1.2 years) taking part in a pre-season conditioning camp were supplemented once a day, for six weeks, with 100 ml of the juice blend. At the start and the end of the camp the athletes performed a 300-m sprint running test on an outdoor track. Blood samples were taken before and immediately after the test and after 1 h of recovery. Blood antioxidant status was evaluated based on activities of antioxidant enzymes (superoxide dismutase [SOD], catalase [CAT], glutathione peroxidase [GSH-Px], glutathione reductase [GR]), concentrations of non-enzymatic antioxidants (reduced glutathione [GSH], uric acid), total plasma polyphenols, ferric reducing ability of plasma (FRAP), thiobarbituric acid reactive substances (TBARS) and activities of creatine kinase (CK) and lactate dehydrogenase (LDH) as muscle damage markers. In order to evaluate potential health benefits of the acai berry, the post-treatment changes in lipid profile parameters (triglycerides, cholesterol and its fractions) were analysed. Six weeks' consumption of acai berry-based juice blend had no effect on sprint performance, but it led to a marked increase in the total antioxidant capacity of plasma, attenuation of the exercise-induced muscle damage, and a substantial improvement of serum lipid profile. These findings strongly support the view of the health benefits of supplementation with the acai berry-based juice blend, mainly attributed to its high total polyphenol content and the related high in vivo antioxidant and hypocholesterolaemic activities of this supplement. PMID:26060341

  11. Phenotyping of leukocytes and granulocyte and monocyte phagocytic activity in the peripheral blood and uterus of cows with endometritis.

    Science.gov (United States)

    Brodzki, P; Kostro, K; Brodzki, A; Lisiecka, U; Kurek, L; Marczuk, J

    2014-08-01

    This study was a comparative evaluation of selected immunological parameters in peripheral blood and uterine wash samples from cows with a normal postpartum period compared with cows with endometritis. We aimed to determine the usefulness of these parameters in monitoring the puerperium. In total, 40 cows were included in the study: 20 had endometritis (experimental group), and 20 did not have uterine inflammation (control group). Animals were chosen on the basis of cytological and bacteriological test results. The tests were conducted 5, 22, and 40 days postpartum. In both groups, flow cytometric analysis of the surface molecules CD4, CD8, CD21, CD25, and CD14 in the peripheral blood and uterine washings was performed. Granulocyte and monocyte phagocytic activity was determined using a commercial Phagotest kit that was adapted for flow cytometry. The percentage of phagocytic granulocytes and monocytes in both the peripheral blood and the uterine washings was significantly lower for cows in the experimental group compared with the control group (P cows with endometritis. A significant decrease (P endometritis. Knowledge of the immunological mechanisms observed in cows with endometritis might aid in choosing the correct immunomodulating agent-based adjuvant therapy. PMID:24857644

  12. COMT Val158Met polymorphism is associated with blood pressure and lipid levels in general families of Bama longevous area in China

    OpenAIRE

    Ge, Lin; Wu, Hua-Yu; Pan, Shang-Ling; Huang, Ling; Sun, Peng; Liang, Qing-Hua; Pang, Guo-Fang; Lv, Ze-ping; Hu, Cai-You; Liu, Cheng-wu; Zhou, Xiao-Ling; Huang, Ling-Jin; Yin, Rui-Xing; Peng, Jun-Hua

    2015-01-01

    To see the possible relationship between COMT Val158Met polymorphism and blood pressure (BP) and serum lipid levels and its putative role in human longevity, we genotyped COMT Val158Met (rs4680) by PCR-RFLP for members from Bama long-lived families (BLF, n = 1538), Bama non-long-lived families (BNLF, n = 600), Pingguo (a county outside Bama region) long-lived families (PLF, n = 538) and Pingguo non-long-lived families (PNLF, n = 403) after anthropometric measures were collected and serum lipi...

  13. Effects of Dietary L-carnosine and Alpha-lipoic Acid on Growth Performance, Blood Thyroid Hormones and Lipid Profiles in Finishing Pigs

    OpenAIRE

    Bao, Yinghui; Gao, Chunqi; Hao, Wenbo; Ji, Cheng; Zhao, Lihong; Zhang, Jianyun; Liu, Tao; Ma, Qiugang

    2015-01-01

    The present study was conducted to determine the effects of L-carnosine (LC) and/or alpha-lipoic acid (ALA) supplementation on growth performance, blood thyroid hormones and lipid profiles in finishing pigs. A total of 40 (Landrace×Yorkshire) pigs with an initial body weight of 57.93±3.14 kg were randomly allocated to 4 experimental diets using a 2×2 factorial arrangement with 2 LC supplemental levels (0 or 0.1%) and 2 ALA supplemental levels (0 or 0.03%) in basal diets. The results showed th...

  14. Evaluation of phenotypic parameters of the mononuclear peripheral blood cells through flow cytometry from dogs submitted to irradiation of head and neck

    International Nuclear Information System (INIS)

    The present paper addresses the investigation of the phenotypic parameters applying stimulation markers and cell immunosuppression markers in an animal model, through a flux cytometer, with the goal of looking for a future monitoring of the immunologic response of patients submitted to radiotherapy. Five dogs were submitted to low dose radiation, 2, 4 and 6 Gy at a protocol of head and neck. Samples of peripheral blood (PBMC) were collected before and after irradiation, and 2, 18h and 30d. Such samples were separated and were submitted to surface markers with monoclonal antibody anti-CD5, anti-CD4, anti-IgM, anti- CD14, anti-MHCII, anti-CTLA-4 and anti-IL-2R. The PBMCs were evaluated by flow cytometry. The following alterations were observed: reduction of the T-cell in circulation (CD5) and of the subpopulation CD4, reduction of the stimulation receptor and cellular interaction (MHC2) and from IL-2R, increasing of the expression CTLA-4. It was proved that the peripheral circulated cells during an in locus irradiation are morphological modified and therefore has its function redefined. Such results show the relevance of knowing the personal immune response of each patient through the investigation of the phenotype patter of their peripheral cell lineage. The quality of life of the patient can be improved and irradiation protocols may be altering in according to the personal immune response picture. (author)

  15. Understanding red blood cell parameters in the context of the frailty phenotype: interpretations of the FIBRA (Frailty in Brazilian Seniors) study.

    Science.gov (United States)

    Silva, João Carlos; Moraes, Zélia Vieira de; Silva, Conceição; Mazon, Silvia de Barros; Guariento, Maria Elena; Neri, Anita Liberalesso; Fattori, André

    2014-01-01

    Frailty and anemia in the elderly appear to share a common pathophysiology associated with chronic inflammatory processes. This study uses an analytical, cross-sectional, population-based methodology to investigate the probable relationships between frailty, red blood cell parameters and inflammatory markers in 255 community-dwelling elders aged 65 years or older. The frailty phenotype was assessed by non-intentional weight loss, fatigue, low grip strength, low energy expenditure and reduced gait speed. Blood sample analyses were performed to determine hemoglobin level, hematocrit and reticulocyte count, as well as the inflammatory variables IL-6, IL-1ra and hsCRP. In the first multivariate analysis (model I), considering only the erythroid parameters, Hb concentration was a significant variable for both general frailty status and weight loss: a 1.0g/dL drop in serum Hb concentration represented a 2.02-fold increase (CI 1.12-3.63) in an individual's chance of being frail. In the second analysis (model II), which also included inflammatory cytokine levels, hsCRP was independently selected as a significant variable. Each additional year of age represented a 1.21-fold increase in the chance of being frail, and each 1-unit increase in serum hsCRP represented a 3.64-fold increase in the chance of having the frailty phenotype. In model II reticulocyte counts were associated with weight loss and reduced metabolic expenditure criteria. Our findings suggest that reduced Hb concentration, reduced RetAbs count and elevated serum hsCRP levels should be considered components of frailty, which in turn is correlated with sarcopenia, as evidenced by weight loss. PMID:25236441

  16. Histo-blood group ABO antigen in oral potentially malignant lesions and squamous cell carcinoma--genotypic and phenotypic characterization

    DEFF Research Database (Denmark)

    Gao, Shan; Bennett, Erik Paul; Reibel, Jesper;

    2004-01-01

    Loss of histo-blood group A/B antigens is frequent in oral cancer. It is unclear whether this alteration is due to loss of the chromosomal region encoding the genes. The aim was to investigate genotypic alterations in the ABO locus in oral potentially malignant lesions and carcinomas. Seventy...... and 3/24 cases with mild and moderate dysplasia by genotyping analysis. O allele loss was found in 10 cases involving all four groups. In patients with heterozygous genotypes, A/B allelic loss by genotyping analysis was always followed by loss of A/B antigen expression by IHC staining. Loss of A...

  17. Intravesical BCG therapy in bladder carcinoma. Effect on cytotoxicity, IL-2 production and phenotype of peripheral blood mononuclear cells

    DEFF Research Database (Denmark)

    Hermann, G G; Petersen, K R; Zeuthen, J; Steven, K

    1991-01-01

    The purpose of the study was to examine the effects of intravesical BCG treatment on the cytotoxicity, interleukin-2 (IL-2) production and distribution of the subsets of peripheral blood mononuclear cells (PBMC) in patients with carcinoma in situ of the bladder. Treatments were made in 6 patients...... during a conventional BCG treatment schedule. Four patients showed a complete response, one a partial response and one had a progressive disease after BCG treatment. Intravesical BCG did not induce significant changes in the cytotoxicity of PBMC. The distribution of NK-cells and T-cells also remained...

  18. Effects of Sesame Oil on Blood Glucose and Lipid Profile in Type II Diabetic Patients Referring to The Yazd Diabetes Research Center.

    Directory of Open Access Journals (Sweden)

    F Hoseini

    2008-07-01

    Full Text Available Introduction: Type II Diabetes is one of the most prevalent endocrine diseases in the world that results from a combination of insulin resistance and ß-cell failure. Regarding importance of nutritional factors in management of diabetes, this study was designed to explore the effect of sesame oil on blood glucose and lipid profile in type II diabetic patients at Yazd Diabetes Research Center in 2007. Methods: A quasi-experimental study was conducted on 25 patients with type II diabetes mellitus (age: 51.5±6.28y; BMI:27.3±3kg/m2; disease duration:7.08±5.03y; Fasting blood glucose level: 181±51.9mg/dl. Subjects received 30 g/day sesame oil for 6 weeks. Sesame oil was supplied to the patients, who were instructed to use it in place of other cooking oils for 42 days. Plasma glucose, glycated hemoglobin (HbA1c, lipid profiles [Total cholesterol (TC, low-density lipoprotein cholesterol (LDL-C, high-density lipoprotein cholesterol (HDL-C and triglycerides (TG] were measured at baseline and after 45 days of sesame oil substitution. 24 hours dietary recalls were obtained at the start , middle and end of study. Data was analyzed using analysis of variance with repeated measures and paired t-test. Results: Following 42 days intake of sesame oil, there were significant decrease in FBS (181±51.93 vs 154±39.65 mg/dl, HbA1c (9.64 ± 2 vs 8.4 ± 1.74 percent, TC (226.68 ± 31.4 vs 199.8 ± 37.87 mg/dl, LDL-c (123.9 ± 34.56 vs 95.53 ± 32.54 mg/dl compared to pre-treatment values. (P <0.05 . Blood TG level decreased after intake of sesame oil but this difference was not significant (P=0.2.Also, the changes of HDL-c levels were not significant (P=0.1. Conclusion: Sesame oil consumption results in considerable decrease in blood sugar, HbA1c and blood lipid levels (TC and LDL-C in type II diabetics.

  19. Effect of 12-week-long aerobic training programme on body composition, aerobic capacity, complete blood count and blood lipid profile among young women

    OpenAIRE

    Kostrzewa-Nowak, Dorota; Nowak, Robert; Jastrzębski, Zbigniew; Zarębska, Aleksandra; Bichowska, Marta; Drobnik-Kozakiewicz, Izabela; Radzimiński, Łukasz; Leońska-Duniec, Agata; Ficek, Krzysztof; Cięszczyk, Paweł

    2015-01-01

    Background: Numerous data suggest that aerobic-type exercise improves lipoprotein-lipid profiles, cardiorespiratory fitness and body composition in young women. The aim of this study was to evaluate the biological response to high-low impact aerobic fitness among young women. Materials and methods: Thirty-four young women aged 22 (19-24) years were divided into three groups: underweight (N = 10), normal weight (N = 12) and overweight (N = 12). Aerobic capacity, anthropometry and body comp...

  20. Effect of Orange (Citrus sinensis Peel Oil on Lipid Peroxidation, Catalase activity and Hepatic Biomarker levels in Blood Plasma of Normo Rats

    Directory of Open Access Journals (Sweden)

    Ochuko L. Erukainure

    2012-07-01

    Full Text Available Dietary antioxidants are considered beneficial because of their potential protective role against oxidative stress, which is involved in the pathogenesis of multiple diseases such as cancer and coronary heart disease. The effect of feeding orange peel oil on lipid peroxidation, catalase and hepatic biomarkers in blood plasma of normo rats was investigated. Beside mouse chow, four diets were designed to contain 50% of energy as carbohydrate, 35% as fat, and 15% as protein, and one that was lipid-free diet which had distilled water substituted for fat. Groups of five rats were each fed one of these diets, while a fifth group was fed pelletized mouse chow. There was no significant difference in the amount of food consumed, though significant weight lost was observed in all groups except soybean oil. Feeding on orange peel oil led to significant (p<0.05 decrease in lipid peroxidation and catalase activities in comparison to soybean oil. Higher AST and lower ALT activities were observed in orange peel oil fed groups. These results suggest the oil from the orange peels possesses antioxidant potentials which could be protective against oxidative stress, thus useful in its treatment and management. However, the elevated levels of hepatic biomarkers pose a threat of hepatotoxicity thus suggesting that it should be consumed or used as a pharmaceutical ingredient at lower concentrations.

  1. Genetic disposition and response of blood lipids to diet - studies on gene-diet interaction in humans

    NARCIS (Netherlands)

    Weggemans, R.M.

    2001-01-01

    Even though a cholesterol-lowering diet is effective for most people, it is not for all. Identification of genetic determinants of the serum lipid response to diet may be of help in the identification of subjects who will not benefit from a cholesterol-lowering diet. It may also clarify the role of

  2. Effects of intensity of aerobics on body composition and blood lipid profile in obese/overweight females

    Directory of Open Access Journals (Sweden)

    Sayyed Mohammad Marandi

    2013-01-01

    Conclusions: Our findings showed that both light and moderate aerobics improved body composition and serum lipid profile in obese/overweight women. Our findings support the application of aerobics for obese/overweight women. Initially, they can start with light programs and proceed to more intense programs.

  3. Effects of low-fat dairy intake on blood pressure, endothelial function, and lipoprotein lipids in subjects with prehypertension or stage 1 hypertension

    Directory of Open Access Journals (Sweden)

    Maki KC

    2013-07-01

    Full Text Available Kevin C Maki,1 Tia M Rains,1 Arianne L Schild,1 Mary R Dicklin,1 Keigan M Park,2 Andrea L Lawless,1 Kathleen M Kelley1 1Biofortis Clinical Research, Addison, IL, USA; 2Dairy Research Institute/National Dairy Council, Rosemont, IL, USA Objective: This randomized crossover trial assessed the effects of 5 weeks of consuming low-fat dairy (one serving/day each of 1% fluid milk, low-fat cheese, and low-fat yogurt versus nondairy products (one serving/day each of apple juice, pretzels, and cereal bar on systolic and diastolic blood pressures (SBP and DBP, vascular function (reactive hyperemia index [RHI] and augmentation index, and plasma lipids. Methods: Patients were 62 men and women (mean age 54.5 years, body mass index 29.2 kg/m2 with prehypertension or stage 1 hypertension (mean resting SBP/DBP 129.8 mmHg/80.8 mmHg while not receiving antihypertensive medications. A standard breakfast meal challenge including two servings of study products was administered at the end of each treatment period. Results: Dairy and nondairy treatments did not produce significantly different mean SBP or DBP in the resting postprandial state or from premeal to 3.5 hours postmeal (SBP, 126.3 mmHg versus 124.9 mmHg; DBP, 76.5 mmHg versus 75.7 mmHg, premeal (2.35 versus 2.20 or 2 hours postmeal (2.33 versus 2.30 RHI, and premeal (22.5 versus 23.8 or 2 hours postmeal (12.4 versus 13.2 augmentation index. Among subjects with endothelial dysfunction (RHI ≤ 1.67; n = 14 during the control treatment, premeal RHI was significantly higher in the dairy versus nondairy condition (2.32 versus 1.50, P = 0.002. Fasting lipoprotein lipid values were not significantly different between treatments overall, or in subgroup analyses. Conclusion: No significant effects of consuming low-fat dairy products, compared with low-fat nondairy products, were observed for blood pressures, measures of vascular function, or lipid variables in the overall sample, but results from subgroup analyses

  4. Lipid profiles of blood serum and fatty acid composition of meat of hybrid duck fed diet supplemented with Noni (Morinda citrifolia fruit meal

    Directory of Open Access Journals (Sweden)

    David Kurniawan

    2015-09-01

    Full Text Available Noni fruit is a medicinal plant with biological activity like antioxidant that could potentially be used as a feed additive in poultry. This research investigated the effect of noni fruit powder as feed additive on lipid profiles of blood and meat fatty acid compositions of meat of hybrid duck. One hundred twenty 2-week-old hybrid ducks crossing between Peking and Khaki Campbell duck were subjected. They were randomly allotted to 24 experimental units. Each experimental unit was 70x80x40 cm in size and it was used for 5 ducks up to they reached 56 days of age. Each unit was equipped with waterer and feeder. The ducks were raised on litter-type floor. The basal experimental diet was formulated according to the standards of National Research Council (1994. The method used for this study was experimental with 4 different treatments in 6 replications. The treatments were as follow: P0: basal feed without supplementation of noni fruit powder as control; P1: basal feed + 1 % noni fruit powder; P2: basal feed + 2 % noni fruit powder; P3: basal feed + 3 % noni fruit powder. Data were analyzed by one-way of Completely Randomized Design ANOVA and if there was significant effect followed by Duncan’s Multiple Range Test. Result showed that using noni fruit powder as feed additive had no significant effect (P>0.05 on lipid profiles of blood and fatty acid composition of meat.

  5. EFFECTS OF CREATINE, GINSENG, AND ASTRAGALUS SUPPLEMENTATION ON STRENGTH, BODY COMPOSITION, MOOD, AND BLOOD LIPIDS DURING STRENGTH-TRAINING IN OLDER ADULTS

    Directory of Open Access Journals (Sweden)

    Michael E. Rogers

    2006-03-01

    Full Text Available The effects of supplemental dietary creatine and a botanical extract consisting of ginseng and astragalus were evaluated in 44 adults aged 55-84 years participating in a 12-week strength-training program. Participants consumed creatine only (Cr, creatine plus botanical extract (CrBE, or placebo (PL, and performed bench press, lat pull down, biceps curl, leg press, knee extension, and knee flexion for 3 sets of 8-12 reps on 3 days per week for 12 weeks. The 1-repetition maximum for each exercise, body composition (full-body DEXA, blood lipids, and mood states were evaluated before and after the intervention. Training improved (p < 0.05 strength and lean mass for all groups, however greater gains were observed with Cr and CrBE compared with placebo (but no difference was found between Cr and CrBE. Only CrBE improved blood lipids and self-reported vigor, and the CrBE group lost significantly more body fat and gained more bench press strength than Cr. These results indicate that strength and lean mass gains achieved by older adults participating in a strength training program can be enhanced with creatine supplementation, and that ginseng and astragalus may provide additional health and psychological benefits. However, these herbs do not appear to have an additive effect on strength and lean mass gains during training

  6. Stimulation of Wnt/ß-catenin pathway in human CD8+ T lymphocytes from blood and lung tumors leads to a shared young/memory phenotype.

    Directory of Open Access Journals (Sweden)

    Marie-Andrée Forget

    Full Text Available Cancer can be treated by adoptive cell transfer (ACT of T lymphocytes. However, how to optimally raise human T cells to a differentiation state allowing the best persistence in ACT is a challenge. It is possible to differentiate mouse CD8(+ T cells towards stem cell-like memory (T(SCM phenotype upon TCR stimulation with Wnt/ß-catenin pathway activation. Here, we evaluated if T(SCM can be obtained from human mature CD8(+ T cells following TCR and Wnt/ß-catenin activation through treatment with the chemical agent 4,6-disubstituted pyrrolopyrimidine (TWS119, which inhibits the glycogen synthase kinase-3β (GSK-3β, key inhibitor of the Wnt pathway. Human CD8(+ T cells isolated from peripheral blood or tumor-infiltrating lymphocytes (TIL, and treated with TWS119 gave rise to CD62L(+CD45RA(+ cells, indicative of early differentiated stage, also expressing CD127 which is normally found on memory cells, and CD133, an hematopoietic stem cell marker. T(SCM cells raised from either TIL or blood secreted numerous inflammatory mediators, but in lower amounts than those measured without TWS119. Finally, generated T(SCM CD8(+ T cells expressed elevated Bcl-2 and no detectable caspase-3 activity, suggesting increased persistence. Our data support a role for Wnt/ß-catenin pathway in promoting the T(SCM subset in human CD8(+ T cells from TIL and the periphery, which are relevant for ACT.

  7. CHANGES IN THE PARAMETERS OF BLOOD LIPIDS AND CYTOKINES IN PATIENTS WITH PRIMARY OSTEOARTHRITIS DURING TREATMENT WITH ARTHRA

    Directory of Open Access Journals (Sweden)

    E Yu Alexenko

    2011-01-01

    Conclusion. The POA patients treated with Arthra displayed improvements in LPO profile and antioxidant defense and lower blood concentrations of cholesterol and LDL cholesterol along with a decline in the levels of the blood cytokines IL-1β, IL-6, IL-18, TNF-α, and CRP.

  8. Orange juice consumption and its effect on blood lipid profile and indices of the metabolic syndrome; a randomised, controlled trial in an at-risk population.

    Science.gov (United States)

    Simpson, E J; Mendis, B; Macdonald, I A

    2016-04-20

    Data from epidemiological and in vitro studies suggest that orange juice (OJ) may have a positive impact on lipid metabolism. However, there have been reports in the media claiming detrimental consequences of 100% juice consumption, including weight-gain and adverse effects on insulin sensitivity and blood lipid profile. The effect of daily OJ consumption was assessed using a randomised, placebo-controlled, single-blinded, parallel group design. Thirty-six overweight, but otherwise healthy men (40-60 years; 27-35 kg m(-2)) with elevated fasting serum cholesterol (5-7 mmol l(-1)), were recruited from the general UK population. None were using nutritional strategies or medication to lower their cholesterol, nor were regular consumers of citrus products. Assessment of BMI, HOMA-IR, and circulating lipid (total cholesterol, low-density lipoprotein, high-density lipoprotein, non-esterified fatty acids, triacylglycerol, apolipoprotein-A1 and apolipoprotein-B) concentrations, was made when fasted before (V1) and after a 12-week intervention (V2), during which participants consumed 250 ml per d of OJ or an energy and sugars-matched orange-flavoured drink (control). The two groups were matched at V1 with respect to all parameters described above. Although triacylglycerol concentration was similar between the groups at both visits, a trend for the change in this variable to differ between groups was observed (P = 0.060), with those in control exhibiting a significant increase in triacylglycerol at V2, compared with V1. In OJ, those with the highest initial triacylglycerol concentration showed the greatest reduction at V2 (R(2) = 0.579; P < 0.001), whereas there was no correlation between these variables in controls (R(2) = 0.023; P = 0.548). Twelve weeks consumption of 250 ml per d of OJ did not adversely affect insulin sensitivity, circulating lipids or body weight. PMID:26965492

  9. Characterization of multi-drug resistant ESBL producing nonfermenter bacteria isolated from patients blood samples using phenotypic methods in Shiraz (Iran

    Directory of Open Access Journals (Sweden)

    Maneli Amin Shahidi

    2015-10-01

    Full Text Available Background and Aim: The emergence of  nonfermenter bacteria that are resistant to multidrug resistant ESBL  are  nowadays a principal problem  for hospitalized patients. The present study aimed at surveying the emergence of nonfermenter bacteria resistant to multi-drug ESBL producing isolated from patients blood samples using BACTEC 9240 automatic system in Shiraz. Materials and Methods: In this cross-sectional study, 4825 blood specimens were collected from hospitalized patients in Shiraz (Iran, and positive samples were detected by means of  BACTEC 9240 automatic system. The isolates  containing nonfermenter bacteria were identified based on biochemical tests embedded in the API-20E system. Antibiotic sensitivity  test was performed  and identification of  ESBL producing strains were done  using phenotypic detection of extended spectrum beta-lactamase producing isolates(DDST according to CLSI(2013 guidelines.   Results: Out of 4825 blood samples, 1145 (24% specimen were gram-positive using BACTEC system. Among all isolated microorganisms, 206 isolates were non-fermenting gram- negative bacteria. The most common non-fermenter isolates were Pseudomonas spp. (48%, Acinetobacter spp. (41.7% ,and Stenotrophomonas spp. (8.2%. Seventy of them (81.4% were  Acinetobacter spp. which were ESBL positive. Among &beta-lactam antibiotics, Pseudomonas spp. showed  the best sensitivity to piperacillin-tazobactam (46.5%.  Conclusion: It was found that  &beta-lactam antibiotics are not effective against more than 40% of Pseudomonas spp. infections and 78% Acinetobacter infections. Emergence of multi-drug resistant strains that are resistant to most antibiotic classes is a major public health problem in Iran. To resolve this problem using of practical guidelines is critical.

  10. Increased efflux of amyloid-β peptides through the blood-brain barrier by muscarinic acetylcholine receptor inhibition reduces pathological phenotypes in mouse models of brain amyloidosis.

    Science.gov (United States)

    Paganetti, Paolo; Antoniello, Katia; Devraj, Kavi; Toni, Nicolas; Kieran, Dairin; Madani, Rime; Pihlgren, Maria; Adolfsson, Oskar; Froestl, Wolfgang; Schrattenholz, André; Liebner, Stefan; Havas, Daniel; Windisch, Manfred; Cirrito, John R; Pfeifer, Andrea; Muhs, Andreas

    2014-01-01

    The formation and accumulation of toxic amyloid-β peptides (Aβ) in the brain may drive the pathogenesis of Alzheimer's disease. Accordingly, disease-modifying therapies for Alzheimer's disease and related disorders could result from treatments regulating Aβ homeostasis. Examples are the inhibition of production, misfolding, and accumulation of Aβ or the enhancement of its clearance. Here we show that oral treatment with ACI-91 (Pirenzepine) dose-dependently reduced brain Aβ burden in AβPPPS1, hAβPPSL, and AβPP/PS1 transgenic mice. A possible mechanism of action of ACI-91 may occur through selective inhibition of muscarinic acetylcholine receptors (AChR) on endothelial cells of brain microvessels and enhanced Aβ peptide clearance across the blood-brain barrier. One month treatment with ACI-91 increased the clearance of intrathecally-injected Aβ in plaque-bearing mice. ACI-91 also accelerated the clearance of brain-injected Aβ in blood and peripheral tissues by favoring its urinal excretion. A single oral dose of ACI-91 reduced the half-life of interstitial Aβ peptide in pre-plaque mhAβPP/PS1d mice. By extending our studies to an in vitro model, we showed that muscarinic AChR inhibition by ACI-91 and Darifenacin augmented the capacity of differentiated endothelial monolayers for active transport of Aβ peptide. Finally, ACI-91 was found to consistently affect, in vitro and in vivo, the expression of endothelial cell genes involved in Aβ transport across the Blood Brain Brain (BBB). Thus increased Aβ clearance through the BBB may contribute to reduced Aβ burden and associated phenotypes. Inhibition of muscarinic AChR restricted to the periphery may present a therapeutic advantage as it avoids adverse central cholinergic effects. PMID:24072071

  11. The formation of short-chain fatty acids is positively associated with the blood lipid-lowering effect of lupin kernel fiber in moderately hypercholesterolemic adults.

    Science.gov (United States)

    Fechner, Anita; Kiehntopf, Michael; Jahreis, Gerhard

    2014-05-01

    Lupin kernel fiber beneficially modifies blood lipids because of its bile acid-binding capacity. The aim of this study was to evaluate the preventive effects of a lupin kernel fiber preparation on cardiovascular diseases and to clarify possible mechanisms. In a randomized, double-blind, controlled crossover trial, 60 moderately hypercholesterolemic adults (plasma total cholesterol: >5.2 mmol/L) passed 3 intervention periods in different orders with a 2-wk washout phase between each. Participants consumed either a high-fiber diet containing 25-g/d lupin kernel fiber (LF) or citrus fiber (CF), or a low-fiber control diet (CD) for 4 wk each. Anthropometric, plasma, and fecal variables were assessed at baseline and after the interventions. Contrary to the CF period, total (9%) and LDL (12%) cholesterol as well as triacylglycerols (10%) were lower after the LF period when compared with the CD period [P ≤ 0.02, adjusted for baseline, age, gender, and body mass index (BMI)]. HDL cholesterol remained unchanged. Moreover, the LF period reduced high-sensitivity C-reactive protein (P = 0.02) and systolic blood pressure (P = 0.01) when compared with baseline. Bile acid binding could not be shown because the excretion of total bile acids remained constant after the high-fiber diets. However, the LF period resulted in an enhanced formation of the main short-chain fatty acids in comparison with the CD period. During the CF period, only acetate increased significantly. Both high-fiber diets led to higher satiety and modified nutritional behavior, resulting in significantly lower body weight, BMI, and waist circumference compared with the CD period. The blood lipid-lowering effects of LF are apparently not a result of bile acid binding. Rather, we hypothesize for the first time, to our knowledge, that the blood lipid-lowering effects of LF may be mainly attributed to the formation of short-chain fatty acids, specifically propionate and acetate. This trial was registered at

  12. Non-genomic inhibitory effect of glucocorticoids on activated peripheral blood basophils through suppression of lipid raft formation.

    Science.gov (United States)

    Yamagata, S; Tomita, K; Sano, H; Itoh, Y; Fukai, Y; Okimoto, N; Watatani, N; Inbe, S; Miyajima, H; Tsukamoto, K; Santoh, H; Ichihashi, H; Sano, A; Sato, R; Tohda, Y

    2012-10-01

    We investigated the non-genomic effects of glucocorticoids (GCs) on inhibition of plasma membrane lipid raft formation in activated human basophils. Human basophils obtained from house dust mite (HDM)-sensitive volunteers were pretreated with hydrocortisone (CORT) or dexamethasone (Dex) for 30 min and then primed with phorbol 12-myristate 13-acetate (PMA, 10 ng/ml) or HDM (10 µg/ml). The expression of CD63, a basophil activation marker, was assessed by flow cytometry. Membrane-bound GC receptors (mGCRs) were analysed by flow cytometry and confocal laser microscopy. Lipid rafts were assessed using a GM1 ganglioside probe and visualization by confocal laser microscopy. Pretreatment of basophils with CORT (10(-4) M and 10(-5) M) and Dex (10(-7) M) significantly inhibited CD63 expression 20 min after addition of PMA or HDM. The inhibitory effects of GCs were not altered by the nuclear GC receptor (GCR) antagonist RU486 (10(-5) M) or the protein synthesis inhibitor cycloheximide (10(-4) M) (P < 0·05). CORT coupled to bovine serum albumin (BSA-CORT) mimicked the rapid inhibitory effects of CORT, suggesting the involvement of mGCRs. mGCRs were detectable on the plasma membrane of resting basophils and formed nanoclusters following treatment with PMA or HDM. Pretreatment of cells with BSA-CORT inhibited the expression of mGCRs and nanoclustering of ganglioside GM1 in lipid rafts. The study provides evidence that non-genomic mechanisms are involved in the rapid inhibitory effect of GCs on the formation of lipid raft nanoclusters, through binding to mGCRs on the plasma membrane of activated basophils. PMID:22943204

  13. Effects of the Dietary Addition of Amaranth (Amaranthus mantegazzianus) Protein Isolate on Antioxidant Status, Lipid Profiles and Blood Pressure of Rats.

    Science.gov (United States)

    Lado, María B; Burini, Julieta; Rinaldi, Gustavo; Añón, María C; Tironi, Valeria A

    2015-12-01

    The effects of the dietary addition of 2.5% (w/w) Amaranthus mantegazzianus protein isolate (AI) on blood pressure, lipid profiles and antioxidative status of Wistar rats were evaluated. Six diets were used to feed animals during 28 days: (base (AIN93G), Chol (cholesterol 1%, w/w), CE (α-tocopherol 0.005%, w/w), CholE (cholesterol 1% (w/w) + α-tocopherol 0.005%, w/w), CAI (AI 2.5% w/w), CholAI (cholesterol 1% (w/w) + AI 2.5%, w/w). Lipid profiles of plasma and liver and faecal cholesterol content were analyzed. Antioxidant status was evaluated by the ferric reducing activity of plasma (FRAP), the 2-thiobarbituric acid (TBA) assay and superoxide dismutase (SOD) activity in plasma and liver. Blood pressure was measured in the tail artery of rats. CholA group presented a significant (α < 0.05) reduction (16%) in the plasma total cholesterol. In liver, the intake of cholesterol (Chol group) induced a significant increment in cholesterol and triglycerides (2.5 and 2.3 times, respectively), which could be decreased (18% and 47%, respectively) by the addition of AI (CholA group). This last group also showed an increased faecal cholesterol excretion (20%). Increment (50%) in FRAP values, diminution of TBA value in plasma and liver (70% and 38%, respectively) and diminution of SOD activity (20%) in plasma of CholA group suggest an antioxidant effect because of the intake of AI. In addition, CA and CholA groups presented a diminution (18%) of blood pressure after 28 days. PMID:26497504

  14. Modulatory effect of pineapple peel extract on lipid peroxidation,catalase activity and hepatic biomarker levels in blood plasma of alcoholinduced oxidative stressed rats

    Institute of Scientific and Technical Information of China (English)

    Okafor; OY; Erukainure; OL; Ajiboye; JA; Adejobi; RO; Owolabi; FO; Kosoko; SB

    2011-01-01

    Objective:To investigate the ability of the methanolic extract of pineapple peel to modulate alcohol-induced lipid peroxidation,changes in catalase activities and hepatic biochemical marker levels in blood plasma.Methods:Oxidative stress was induced by oral administration of ethanol(20%w/v) at a dosage of 5 niL/kg bw in rats.After 28 days of treatment,the rats were fasted overnight and sacrificed by cervical dislocation.Blood was collected with a 2 mL syringe by cardiac puncture and was centrifuged at 3000 rpm for 10 min.The plasma was analyzed to evaluate malondialdehyde(MDA),catalase activity,aspartate aminotransferase(AST),alkaline phosphatase(ALP) and alanine aminotransferase(ALT) concentrations.Results:Administration of alcohol caused a drastic increase(87.74%) in MDA level compared with the control.Pineapple peel extract significantly reduced the MDA level by 60.16%at 2.S mL/kg bw.Rats fed alcohol only had the highest catalase activity,treatment with pineapple peel extract at 2.5 mL/kg bw however, reduced the activity.Increased AST,ALP and ALT activities were observed in rats fed alcohol only respectively,treatment with pineapple peel extract drastically reduced their activities. Conclusions:The positive modulation of lipid peroxidation,catalase activities as well as hepatic biomarker levels of blood plasma by the methanolic extract of pineapple peels under alcoholinduced oxidative stress is an indication of its protective ability in the management of alcoholinduced toxicity.

  15. Assessment risk of osteoporosis in Chinese people: relationship among body mass index, serum lipid profiles, blood glucose, and bone mineral density

    Science.gov (United States)

    Cui, Rongtao; Zhou, Lin; Li, Zuohong; Li, Qing; Qi, Zhiming; Zhang, Junyong

    2016-01-01

    Objective The aim of our study was to investigate the relationship among age, sex, body mass index (BMI), serum lipid profiles, blood glucose (BG), and bone mineral density (BMD), making an assessment of the risk of osteoporosis. Materials and methods A total of 1,035 male and 3,953 female healthy volunteers (aged 41–95 years) were recruited by an open invitation. The basic information, including age, sex, height, weight, waistline, hipline, menstrual cycle, and medical history, were collected by a questionnaire survey and physical examination. Serum lipid profiles, BG, postprandial blood glucose, and glycosylated hemoglobin were obtained after 12 hours fasting. BMD in lumbar spine was measured by dual-energy X-ray absorptiometry scanning. Results The age-adjusted BMD in females was significantly lower than in males. With aging, greater differences of BMD distribution exist in elderly females than in males (P7.0 mmol/L was lower than in people with BG of ≤7.0 mmol/L (P<0.001). People with serum high-density lipoprotein cholesterol levels of ≥1.56 mmol/L had a greater prevalence of osteoporosis compared with high-density lipoprotein cholesterol ≤1.55 mmol/L. Logistic regression with odds ratios showed that no association was found among total cholesterol, triglyceride, low-density lipoprotein cholesterol, glycosylated hemoglobin, postprandial blood glucose and BMD. Conclusion The present study further confirmed that factors such as age, sex, weight, BMI, high-density lipoprotein cholesterol, and diabetes are significant predictors of osteoporosis in the Chinese people. PMID:27445467

  16. Effect of squash seed meal (Cucurbita moschata on broiler performance, sensory meat quality, and blood lipid profile

    Directory of Open Access Journals (Sweden)

    YM Aguilar

    2011-12-01

    Full Text Available In this experiment, 240 Cobb-500® broilers reared from1 to 49 days, and distributed according to a completely experimental randomized design with four treatments of four replicates each in order to evaluate the effect of the dietary inclusion of 0, 33, 66 or 100 g/kg of squash seed meal (SSM (Cucurbita moschata on the performance, carcass yield, serum lipid profile and sensory meat quality of broilers. Significant differences (p<0.05 were detected in performance, carcass weight, weight and breast yield, and leg weight. The best results were obtained with 33 and 66 g/kg as compared to the control diet and 100 g SSM /kg. Abdominal fat decreased with the inclusion of 66 and 100 g SSM / kg, but the sensory quality of breast and thighs was not affected by the inclusion of SSM. The serum levels of total cholesterol, very low density (VLDL and low density (LDL lipoproteins, triglycerides, glucose and atherogenic index decreased with the inclusion of 100 g/kg of SSM, except for high density lipoproteins (HDL, which increased. The inclusion of 0, 33, 66 and 100 g/kg of SSM in broiler diets, partially replacing soybean meal and vegetable oil, improved live performance and edible portions yield. In addition, abdominal fat and serum levels of harmful lipids were reduced, whereas serum levels of beneficial lipids increased. There was no effect on meat sensory quality.

  17. Effects of medium-chain fatty acids and oleic acid on blood lipids, lipoproteins, glucose, insulin, and lipid transfer protein activities

    DEFF Research Database (Denmark)

    Tholstrup, T.; Ehnholm, C.; Jauhiainen, M.; Petersen, M.; Høy, Carl-Erik; Lund, Pia; Sandstrøm, B.

    2004-01-01

    Background: Dietary medium-chain fatty acids (MCFAs) are of nutritional interest because they are more easily absorbed from dietary medium-chain triacylglycerols (MCTs) than are long-chain fatty acids from, for example, vegetable oils. It has generally been claimed that MCFAs do not increase plasma...... design, 17 healthy young men replaced part of their habitual dietary fat intake with 70 g MCTs (66% 8:0 and 34% 10:0) or high-oleic sunflower oil (89.4% 18:1). Each intervention period lasted 21 d, and the 2 periods were separated by a washout period of 2 wk. Blood samples were taken before and after the...... intervention periods. Results: Compared with the intake of high-oleic sunflower oil, MCT intake resulted in 11% higher plasma total cholesterol (P = 0.0005), 12% higher LDL cholesterol (P = 0.0001), 32% higher VLDL cholesterol (P = 0.080), a 12% higher ratio of LDL to HDL cholesterol (P = 0.002), 22% higher...

  18. Effects of tanshinone Ⅱ sodium sulfonate plus cinepazide maleate on the hemorrheologic indexes and blood lipids in patients with acute cerebral infarction

    Institute of Scientific and Technical Information of China (English)

    Chunliang Li; Mingzhu Zhang; Haijun Zheng; Fengzhi Xue

    2007-01-01

    BACKGROUND: The severity of cerebral infarction is associated with the increase of blood viscosity caused by hyperfibrinogenemia and hyperlipidemia, etc. Thus it has become one of the target for treating cerebral infarction to decrease blood viscosity by integrated Chinese and western medicine.OBJECTIVE: To investigate the influence and clinical therapeutic effects of cinepazide maleate combined with tanshinone Ⅱ A sodium sulfonate on the hemorrheologic indexes and blood lipids of patients with acute cerebral infarction, and compare the results with those of simple cinepazide maleate treatment.DESIGN: A non-randomized case-controlled observation.SETTINGS: Hebei North University; the Second Affiliated Hospitals of Hebei North University; the Third Affiliated Hospitals of Hebei North University.PARTICIPANTS: Eighty-six inpatients with cerebral infarction were selected from the infirmary, the Second and Third Affiliated Hospitals of Hebei North University from September 2004 to October 2006.They were all diagnosed to have acute cerebral infarction by CT or MRI, and accorded with the diagnostic standards for acute cerebral infarction set by the Fourth National Academic Meeting for Cerebrovascular Disease in 1995. Meanwhile, 40 teachers and medical staff of voluntary physical examinees were selected as the control group. Informed contents were obtained from all the patients and their relatives.METHODS: The patients were divided into combined treatment group (n=43) and simple treatment group (n=43). In the combined treatment group, the patients were administrated with 160 mg cinepazide maleate injection (Beijing Four-ring Pharmaceutical, Co.,Ltd, No. H200220125; 80 mg/2 mL) added in 5% glucose,and 40 mg tanshinone Ⅱ sodium sulfonate (Shanghai No.1 Biochemical & Pharmaceutical Co.,Ltd., No.H31022558, 10 mg/2 mL) added in 250 mL normal saline. In the simple treatment group, the patients were only administrated with cinepazide maleate 320 mg added in 5% glucose or

  19. The Adjustion of Tartary Buckwheat Extracts to Blood Sugar and Blood Lipid of Mouse and Big Rat%苦荞提取物对大小鼠血糖、血脂的调节

    Institute of Scientific and Technical Information of China (English)

    林汝法; 周运宁; 王瑞

    2001-01-01

    The influence of tartary buckwheat extra cts (TBE) on blood sugarof hyperglycemia rat caused by tetraoxygen pyrimidine a nd blood lipid of hyperlipidemia animal were observed.The results showed that TBE had no influence on the blood sugar of normal rats, high and middle dose of TBE could significantly decrease blood sugar of tetraoxygen pyrimidine caused hyperglycemia big rats ,and improved sugar tolera nt dose of tetraoxygen pyrimidine caused hynerglycemia.High dose of TBE could s ignificantly decrease the concentration of total cholesterol of blood serum of rat,and middle dose of TBE could significantly decrease the concentration of total triglyceride of blood serum of rat.%试验观察了苦荞提取物对四氧嘧啶所引起的高血糖型大鼠的降血糖作用及对高血脂症动物血脂的影响。结果表明,苦荞提取物对正常大鼠血糖无影响,大、中剂量对四氧嘧啶所致的高血糖大鼠空腹血糖有明显的降低作用,并改善由四氧嘧啶所引起的高血糖大鼠的糖耐量。给予大剂量苦荞提取物可使大鼠血清总胆固醇(TC)浓度显著下降,给予中剂量可使大鼠血清甘油三酯(TG)浓度显著下降。

  20. Extracellular water and blood pressure in adults with growth hormone (GH deficiency: a genotype-phenotype association study.

    Directory of Open Access Journals (Sweden)

    Edna J L Barbosa

    Full Text Available Growth hormone deficiency (GHD in adults is associated with decreased extracellular water volume (ECW. In response to GH replacement therapy (GHRT, ECW increases and blood pressure (BP reduces or remains unchanged. Our primary aim was to study the association between polymorphisms in genes related to renal tubular function with ECW and BP before and 1 year after GHRT. The ECW measures using bioimpedance analysis (BIA and bioimpedance spectroscopy (BIS were validated against a reference method, the sodium bromide dilution method (Br(-.Using a candidate gene approach, fifteen single-nucleotide polymorphisms (SNPs in nine genes with known impact on renal tubular function (AGT, SCNN1A, SCNN1G, SLC12A1, SLC12A3, KCNJ1, STK39, WNK1 and CASR were genotyped and analyzed for associations with ECW and BP at baseline and with their changes after 1 year of GHRT in 311 adult GHD patients. ECW was measured with the Br(-, BIA, and BIS.Both BIA and BIS measurements demonstrated similar ECW results as the reference method. At baseline, after adjustment for sex and BMI, SNP rs2291340 in the SLC12A1 gene was associated with ECW volume in GHD patients (p = 0.039. None of the SNPs influenced the ECW response to GHRT. One SNP in the SLC12A3 gene (rs11643718; p = 0.024 and three SNPs in the SCNN1G gene [rs5723 (p = 0.02, rs5729 (p = 0.016 and rs13331086 (p = 0.035] were associated with the inter-individual differences in BP levels at baseline. A polymorphism in the calcium-sensing receptor (CASR gene (rs1965357 was associated with changes in systolic BP after GHRT (p = 0.036. None of these associations remained statistically significant when corrected for multiple testing.The BIA and BIS are as accurate as Br(- to measure ECW in GHD adults before and during GHRT. Our study provides the first evidence that individual polymorphisms may have clinically relevant effects on ECW and BP in GHD adults.

  1. body mass (ID 1412), reduction of post-prandial glycaemic responses (ID 1414), and maintenance of a normal blood lipid profile (1421) pursuant to Article 13(1) of Regulation (EC) No 1924/2006

    DEFF Research Database (Denmark)

    Tetens, Inge

    claims in relation to very low calorie diets (VLCDs) and reduction in body weight, reduction in the sense of hunger, reduction in body fat mass while maintaining lean body mass, reduction of post-prandial glycaemic responses, and maintenance of normal blood lipid profile. The scientific substantiation is...... 1412), very low calorie diet is not sufficiently characterised in relation to: reduction of post-prandial glycaemic responses (ID 1414) and maintenance of normal blood lipid profile (ID 1421), mainly owing to the lack of standardisation of the type of available carbohydrates and of most of the fatty...... profile (ID 1421)....

  2. Effects of Intensity of Aerobics on Body Composition and Blood Lipid Profile in Obese/Overweight Females

    OpenAIRE

    Sayyed Mohammad Marandi; Neda Ghadiri Bahram Abadi; Fahimeh Esfarjani; Hosein Mojtahedi; Gholamali Ghasemi

    2013-01-01

    Background: Obesity is a threat to the health of modern urban citizens, especially women. Aerobic is an effect of exercise which rapidly is developing among urban women. This study was designed to examine the effects of light and moderate aerobic intensity on body composition and serum lipid profile in obese/overweight women living in Isfahan. Methods: Forty-five middle-aged obese/overweight volunteer women (25-40 years, and body mass index (BMI) ΃25 to 30 kg/m 2 ) were randomly assigned i...

  3. Human Brain Microvascular Endothelial Cells Derived from the BC1 iPS Cell Line Exhibit a Blood-Brain Barrier Phenotype.

    Science.gov (United States)

    Katt, Moriah E; Xu, Zinnia S; Gerecht, Sharon; Searson, Peter C

    2016-01-01

    The endothelial cells that form capillaries in the brain are highly specialized, with tight junctions that minimize paracellular transport and an array of broad-spectrum efflux pumps that make drug delivery to the brain extremely challenging. One of the major limitations in blood-brain barrier research and the development of drugs to treat central nervous system diseases is the lack of appropriate cell lines. Recent reports indicate that the derivation of human brain microvascular endothelial cells (hBMECs) from human induced pluripotent stem cells (iPSCs) may provide a solution to this problem. Here we demonstrate the derivation of hBMECs extended to two new human iPSC lines: BC1 and GFP-labeled BC1. These hBMECs highly express adherens and tight junction proteins VE-cadherin, ZO-1, occludin, and claudin-5. The addition of retinoic acid upregulates VE-cadherin expression, and results in a significant increase in transendothelial electrical resistance to physiological values. The permeabilities of tacrine, rhodamine 123, and Lucifer yellow are similar to values obtained for MDCK cells. The efflux ratio for rhodamine 123 across hBMECs is in the range 2-4 indicating polarization of efflux transporters. Using the rod assay to assess cell organization in small vessels and capillaries, we show that hBMECs resist elongation with decreasing diameter but show progressive axial alignment. The derivation of hBMECs with a blood-brain barrier phenotype from the BC1 cell line highlights that the protocol is robust. The expression of GFP in hBMECs derived from the BC1-GFP cell line provides an important new resource for BBB research. PMID:27070801

  4. Characterization of Regenerative Phenotype of Unrestricted Somatic Stem Cells (USSC) from Human Umbilical Cord Blood (hUCB) by Functional Secretome Analysis.

    Science.gov (United States)

    Schira, Jessica; Falkenberg, Heiner; Hendricks, Marion; Waldera-Lupa, Daniel M; Kögler, Gesine; Meyer, Helmut E; Müller, Hans Werner; Stühler, Kai

    2015-10-01

    Stem cell transplantation is a promising therapeutic strategy to enhance axonal regeneration after spinal cord injury. Unrestricted somatic stem cells (USSC) isolated from human umbilical cord blood is an attractive stem cell population available at GMP grade without any ethical concerns. It has been shown that USSC transplantation into acute injured rat spinal cords leads to axonal regrowth and significant locomotor recovery, yet lacking cell replacement. Instead, USSC secrete trophic factors enhancing neurite growth of primary cortical neurons in vitro. Here, we applied a functional secretome approach characterizing proteins secreted by USSC for the first time and validated candidate neurite growth promoting factors using primary cortical neurons in vitro. By mass spectrometric analysis and exhaustive bioinformatic interrogation we identified 1156 proteins representing the secretome of USSC. Using Gene Ontology we revealed that USSC secretome contains proteins involved in a number of relevant biological processes of nerve regeneration such as cell adhesion, cell motion, blood vessel formation, cytoskeleton organization and extracellular matrix organization. We found for instance that 31 well-known neurite growth promoting factors like, e.g. neuronal growth regulator 1, NDNF, SPARC, and PEDF span the whole abundance range of USSC secretome. By the means of primary cortical neurons in vitro assays we verified SPARC and PEDF as significantly involved in USSC mediated neurite growth and therewith underline their role in improved locomotor recovery after transplantation. From our data we are convinced that USSC are a valuable tool in regenerative medicine as USSC's secretome contains a comprehensive network of trophic factors supporting nerve regeneration not only by a single process but also maintained its regenerative phenotype by a multitude of relevant biological processes. PMID:26183719

  5. The use of antioxidative stress enzymes, lipid peroxidation, and red blood cell abnormalities as biomarkers of stress in Periphthalmus papilio of the polluted coastal Lagos lagoon.

    Science.gov (United States)

    Nnamdi, Amaeze H; Olumide, Adebesin A; Adeladun, Adepegba E; Oyenike, Kolapo; Rosemary, Egonmwan I

    2015-03-01

    We assessed the mudskipper, Periphthalmus papilio inhabiting the coast line of the Lagos lagoon, Gulf of Guinea, to determine suitable biomarkers of stress due to its current status as a polluted water body. The gill and liver samples showed evidence of some activities of antioxidative stress enzymes including catalase, superoxide dismutase, glutathione-s-transferase, reduced glutahthione, as well as some detectable levels of lipid peroxidation product. The stress status of the fishes was also elucidated by nuclear abnormalities especially micronucleus formation and the presence of numerous vacuolated red blood cells. Given the current need for more sensitive bioindicators in monitoring pollution in this lagoon, we hereby present these inherent responses in P. papilio as a suitable candidate for incorporation into the current repertoire for ecotoxicological investigations in polluted water bodies of the Gulf of Guinea coastline. PMID:25666650

  6. Effects of the extract from roasted chicory (Cichorium intybus L.) root containing inulin-type fructans on blood glucose, lipid metabolism, and fecal properties.

    Science.gov (United States)

    Nishimura, Mie; Ohkawara, Tatsuya; Kanayama, Toshiyuki; Kitagawa, Kazuya; Nishimura, Hiroyuki; Nishihira, Jun

    2015-07-01

    The extract from roasted chicory (Cichorium intybus L.; jú jù) root (chicory root extract), which contains inulin-type fructans, has favorable effects including antihyperglycemic and antidyslipidemic effects and the improvement of bowel movement. In this study, we examined the effects of chicory root extract on blood glucose, lipid metabolism, and fecal properties in 47 healthy adult participants in a randomized, double-blind, placebo-controlled study. The participants were divided into a test group that drank chicory root extract and a placebo group that drank nonchicory root extract (ingesting 300 mL daily for 4 weeks). We performed hematological examinations and body composition measurements, and administered a visual analog scale (VAS) questionnaire for fecal properties at the baseline (Week 0) and after the intervention (Week 4) for the two groups. Although no significant differences in fasting plasma glucose or insulin were observed, hemoglobin A1c was found to decrease by ingesting chicory root extract. No intergroup differences in the levels of lipid metabolism parameters were observed. However, the level of adiponectin was significantly improved in the chicory root extract group when the baseline and postintervention values were compared. In addition, chicory root extract tends to improve the VAS score for fecal properties. These results suggest that chicory root extract could delay or prevent the early onset of diabetes mellitus and improve bowel movements. PMID:26151029

  7. Effect of homocysteine-lowering nutrients on blood lipids: Results from four randomised, placebo-controlled studies in healthy humans

    NARCIS (Netherlands)

    Olthof, M.R.; Vliet, T. van; Verhoef, P.; Zock, P.L.; Katan, M.B.

    2005-01-01

    Background: Betaine (trimethylglycine) lowers plasma homocysteine, a possible risk factor for cardiovascular disease. However, studies in renal patients and in obese individuals who are on a weight-loss diet suggest that betaine supplementation raises blood cholesterol; data in healthy individuals a

  8. Effect of Homocysteine-Lowering Nutrients on Blood Lipids: Result from Four Randomised, Placebo-Controlled Studies in Healthy Humans

    NARCIS (Netherlands)

    Olthof, M.R.; Vliet, van T.; Verhoef, P.; Zock, P.L.; Katan, M.B.

    2005-01-01

    Background Betaine (trimethylglycine) lowers plasma homocysteine, a possible risk factor for cardiovascular disease. However, studies in renal patients and in obese individuals who are on a weight-loss diet suggest that betaine supplementation raises blood cholesterol; data in healthy individuals ar

  9. Evaluation of the effects of Citrus sinensis seed oil on blood glucose, lipid profile and liver enzymes in rats injected with alloxan monohydrate

    Institute of Scientific and Technical Information of China (English)

    Chilaka K.C; Ifediba E.C; Ogamba J.O

    2015-01-01

    Objective: To evaluate the effects of Citrus sinensis seed oil on blood glucose, lipid profile and some liver enzymes activities in alloxan induced diabetic rats.Methods:About 120 mg/kg body weight alloxan monohydrate was injected intraperitoneally into 18 adult male albino rats weighing 180-200 g, which has been acclimatized in our laboratory for two weeks. Approximately 72 h after the alloxan injection, the rat became hyperglycaemic with blood glucose above 200 mg/dL. The diabetic rats were randomly assigned into three diabetic and one control groups of six rats each: normal control, diabetic treated with 1000 mg/kg body weight of emulsified seed oil; diabetic control, diabetic treated with 150 mg/kg body weight of metformin hydrochloride. Both controls received weight-checked solution of 4.8% v/v Tween-80 in distilled water. All injections in all groups were done intraperitoneally once daily for 28 d. The blood glucose estimation was done every week, with one touch glucometer as well as the weight checked with animal weighing balance. Lipid profiles and some liver enzymes activities (AST, ALT and ALP) were analysed using test kits and spectrophotometer. Data obtained were analyzed using One way ANOVA and post hoc test done using graph pad prism-version 6. Results: The results of this study indicated that Citrus sinensis seed oil was able to reduce blood glucose significantly (P<0.001) in the early weeks of the study when compared with both the diabetic control group and the metformin-treated group. The seed oil significantly lowered serum triglyceride, the serum LDL-cholesterol, total cholesterol and VLDL-cholesterol; the activities of all the liver enzymes assayed (P<0.05) but significantly increased the HDL-cholesterol in the diabetic oil-treated rats as compared to diabetic control (P<0.05). Conclusions: However, further studies need to be carried out to show its mechanism of action and to isolate the active ingredient in the Citrus sinensis seed oil that is

  10. Haloperidol-loaded lipid-core polymeric nanocapsules reduce DNA damage in blood and oxidative stress in liver and kidneys of rats

    International Nuclear Information System (INIS)

    Haloperidol (HP) nanoencapsulation improves therapeutic efficacy, prolongs the drug action time, and reduces its motor side effects. However, in a view of HP toxicity in organs like liver and kidneys in addition to the lack of knowledge regarding the toxicity of polymeric nanocapsules, our aim was to verify the influence of HP-nanoformulation on toxicity and oxidative stress markers in the liver and kidneys of rats, also observing the damage caused in the blood. For such, 28 adult male Wistar rats were designated in four experimental groups (n = 7) and treated with vehicle (C group), free haloperidol suspension (FH group), blank nanocapsules suspension (B-Nc group), and haloperidol-loaded lipid-core nanocapsules suspension (H-Nc group). The nanocapsules formulation presented the size of approximately 250 nm. All suspensions were administered to the animals (0.5 mg/kg/day-i.p.) for a period of 28 days. Our results showed that FH caused damage in the liver, evidenced by increased lipid peroxidation, plasma levels of aspartate aminotransferase, and alanine aminotransferase, as well as decreased cellular integrity and vitamin C levels. In kidneys, FH treatment caused damage to a lesser extent, observed by decreased activity of δ-aminolevulinate dehydratase (ALA-D) and levels of VIT C. In addition, FH treatment was also related to a higher DNA damage index in blood. On the other hand, animals treated with H-Nc and B-Nc did not show damage in liver, kidneys, and DNA. Our study indicates that the nanoencapsulation of haloperidol was able to prevent the sub-chronic toxicity commonly observed in liver, kidneys, and DNA, thus reflecting a pharmacological superiority in relation to free drug

  11. Association between altered lipid profile, body mass index, low plasma adiponectin and varied blood pressure in trinidadian type 2 diabetic and non-diabetic subjects

    Directory of Open Access Journals (Sweden)

    Shivananda B Nayak

    2012-01-01

    Full Text Available Background: The obesity and hypertension have become the causes for the development type 2 diabetes. There is a limited study done on the contribution of body mass index (BMI to blood pressure (BP in the Caribbean population. Aim of our study was to determine the associations between lipid profile, BMI, adiponectin, and BP in Trinidadian type 2 diabetic patients with regards to age and ethnicity. Materials and Methods: This was a cohort study comprised of 266 subjects (85 males and 181 females attending primary and tertiary healthcare settings in central Trinidad. Of which, 126 diabetic subjects were matched with 140 non-diabetic subjects. Along with clinical history and anthropometry, adiponectin and lipid profile were measured in fasting blood samples. Results: The diabetic group had higher triglycerides, very low density lipoprotein (VLDL, and BP values which were statistically significant (P < 0.05 when compared to non-diabetic subjects. The high-density lipoprotein cholesterol (HDL-c and adiponectin were lower in diabetic subjects. HDL-c showed significant changes for ethnicity (P = 0.013 and gender (P = 0.043. The mean adiponectin concentrations were found to be significantly different among the ethnic groups (P = 0.001. Systolic pressure varied significantly with age (P = 0.018. As age increased, BP also increased. Ethnic groups had a significant difference in diastolic pressure (P = 0.027. East Indians had the highest mean diastolic pressure (80.74 ± 10.29 when compared to all other ethnic groups. Conclusion: HDL-cholesterol, low levels of adiponectin, and varied BP are associated in Trinidadian type 2 diabetic subjects with regards to age, gender, and ethnicity.

  12. Fermented and extruded wheat bran in piglet diets: impact on performance, intestinal morphology, microbial metabolites in chyme and blood lipid radicals.

    Science.gov (United States)

    Kraler, Manuel; Schedle, Karl; Schwarz, Christiane; Domig, Konrad J; Pichler, Martin; Oppeneder, Alexander; Wetscherek, Wolfgang; Prückler, Michael; Pignitter, Marc; Pirker, Katharina F; Somoza, Veronika; Heine, Daniel; Kneifel, Wolfgang

    2015-01-01

    The aim of the present study was to evaluate the influence of native, fermented and extruded wheat bran on the performance and intestinal morphology of piglets. Additionally, short-chain fatty acids (SCFA), biogenic amines, ammonia, lactic acid, pH as well as E. coli and lactic acid bacterial counts were analysed in digesta samples from three gut sections. Furthermore, the antioxidant potential in blood samples was evaluated based on the lipid radicals formed. For this purpose, 48 newly weaned piglets (28 d old) were allocated to one of the four different dietary treatment groups: no wheat bran (Control), native wheat bran, fermented wheat bran as well as extruded wheat bran. Wheat bran variants were included at 150 g/kg into the diets. All diets were mixed to reach the calculated isonitrogenic nutrient contents. Gut tissue and digesta samples were collected from the proximal jejunum, the terminal ileum and the colon ascendens, blood samples directly at slaughter. Although none of the dietary interventions had an impact on performance parameters, the amount of goblet cells in the ileum was increased upon feeding native and extruded wheat bran, compared to fermented bran (p intestine and therefore less unexploited digesta is available as substrate for the microorganisms there. Fermentation also resulted in a significant decrease of methylamine in the colon (p < 0.05), while other biogenic amines in the ileum and colon showed no statistically significant differences. The formation of lipid radicals was decreased (p < 0.05) after feeding native wheat bran compared to the Control group. These results suggest that fermentation and extrusion of wheat bran exert some different impact regarding their physiological mode of action. PMID:26305386

  13. Haloperidol-loaded lipid-core polymeric nanocapsules reduce DNA damage in blood and oxidative stress in liver and kidneys of rats

    Energy Technology Data Exchange (ETDEWEB)

    Roversi, Katiane, E-mail: katianeroversi@gmail.com [Universidade Federal de Santa Maria, Programa de Pós-Graduação em Farmacologia (Brazil); Benvegnú, Dalila M., E-mail: dalilabenvegnu@yahoo.com.br [Universidade Federal da Fronteira Sul (UFFS), Bioquímica e Farmacologia (Brazil); Roversi, Karine, E-mail: karineroversi-@hotmail.com [Universidade Federal de Santa Maria (UFSM), Departamento de Fisiologia e Farmacologia, Centro de Ciências da Saúde (Brazil); Trevizol, Fabíola, E-mail: fatrevizol@yahoo.com.br [Universidade Federal de Santa Maria, Programa de Pós-Graduação em Farmacologia (Brazil); Vey, Luciana T., E-mail: luciana.taschetto@hotmail.com [Universidade Federal de Santa Maria (UFSM), Departamento de Fisiologia e Farmacologia, Centro de Ciências da Saúde (Brazil); Elias, Fabiana, E-mail: fabiana.elias@uffs.edu.br [Universidade Federal da Fronteira Sul (UFFS), Bioquímica e Farmacologia (Brazil); Fracasso, Rafael, E-mail: rafael.fra@hotmail.com [Universidade Federal do Rio Grande do Sul, Programa de Pós-Graduação em Ciências Farmacêuticas (Brazil); and others

    2015-04-15

    Haloperidol (HP) nanoencapsulation improves therapeutic efficacy, prolongs the drug action time, and reduces its motor side effects. However, in a view of HP toxicity in organs like liver and kidneys in addition to the lack of knowledge regarding the toxicity of polymeric nanocapsules, our aim was to verify the influence of HP-nanoformulation on toxicity and oxidative stress markers in the liver and kidneys of rats, also observing the damage caused in the blood. For such, 28 adult male Wistar rats were designated in four experimental groups (n = 7) and treated with vehicle (C group), free haloperidol suspension (FH group), blank nanocapsules suspension (B-Nc group), and haloperidol-loaded lipid-core nanocapsules suspension (H-Nc group). The nanocapsules formulation presented the size of approximately 250 nm. All suspensions were administered to the animals (0.5 mg/kg/day-i.p.) for a period of 28 days. Our results showed that FH caused damage in the liver, evidenced by increased lipid peroxidation, plasma levels of aspartate aminotransferase, and alanine aminotransferase, as well as decreased cellular integrity and vitamin C levels. In kidneys, FH treatment caused damage to a lesser extent, observed by decreased activity of δ-aminolevulinate dehydratase (ALA-D) and levels of VIT C. In addition, FH treatment was also related to a higher DNA damage index in blood. On the other hand, animals treated with H-Nc and B-Nc did not show damage in liver, kidneys, and DNA. Our study indicates that the nanoencapsulation of haloperidol was able to prevent the sub-chronic toxicity commonly observed in liver, kidneys, and DNA, thus reflecting a pharmacological superiority in relation to free drug.

  14. Effect of Homocysteine-Lowering Nutrients on Blood Lipids: Result from Four Randomised, Placebo-Controlled Studies in Healthy Humans

    OpenAIRE

    Margreet R Olthof; Trinette van Vliet; Petra Verhoef; Peter L Zock; Katan, Martijn B.

    2005-01-01

    Background: Betaine (trimethylglycine) lowers plasma homocysteine, a possible risk factor for cardiovascular disease. However, studies in renal patients and in obese individuals who are on a weight-loss diet suggest that betaine supplementation raises blood cholesterol; data in healthy individuals are lacking. Such an effect on cholesterol would counteract any favourable effect on homocysteine. We therefore investigated the effect of betaine, of its precursor choline in the form of phosphatid...

  15. 糖化血红蛋白与血糖、血脂及心电图异常的相关性分析%Correlation analysis of glycosylated hemoglobin, blood glucose, blood lipid and electrocardiographic abnormality

    Institute of Scientific and Technical Information of China (English)

    王晶

    2014-01-01

    Objective To investigate the relationship between glycosylated hemoglobin (HbA1c), blood glucose, blood lipid and electrocardiographic abnormality, in order to reveal the influence of blood glucose on atherosclerosis.Methods According to the 1999 WHO diagnostic criteria for diabetes and the results of physical examination of 408 subjects, the subjects were divided into normal blood glucose group and abnormal glucose metabolism group. According to the level of HbA1c, the abnormal glucose metabolism group was divided into three groups as group A, group B, and group C, they were HbA1c0.05). The difference of high-density lipoprotein cholesterol (HDL-C) between group A and group B was statistically significant (P0.05). The differences between group B and group C were statistically significant (P0.05),高密度脂蛋白(HDL-C)在A组与B 组间差异具有统计学意义(P0.05),在B 组与 C组间差异具有统计学意义(P<0.05)。分析组和对照组比较, HbA1c 、FBG、PBG、HDL-C、CHOL、TG以及心电图异常发生率各项差异均具有统计学意义(P<0.05)。结论血糖升高可导致血脂异常,从而导致动脉粥样硬化,血糖升高可产生“代谢记忆效应”,应该重视血糖监测以便尽早发现糖代谢异常而进行尽早干预,以减少糖尿病和动脉粥样硬化的发生。

  16. Further phenotypic characterization of the primitive lineage− CD34+CD38−CD90+CD45RA− hematopoietic stem cell/progenitor cell sub-population isolated from cord blood, mobilized peripheral blood and patients with chronic myelogenous leukemia

    International Nuclear Information System (INIS)

    The most primitive hematopoietic stem cell (HSC)/progenitor cell (PC) population reported to date is characterized as being Lin−CD34+CD38−CD90+CD45R. We have a long-standing interest in comparing the characteristics of hematopoietic progenitor cell populations enriched from normal subjects and patients with chronic myelogenous leukemia (CML). In order to investigate further purification of HSCs and for potential targetable differences between the very primitive normal and CML stem/PCs, we have phenotypically compared the normal and CML Lin−CD34+CD38−CD90+CD45RA− HSC/PC populations. The additional antigens analyzed were HLA-DR, the receptor tyrosine kinases c-kit and Tie2, the interleukin-3 cytokine receptor, CD33 and the activation antigen CD69, the latter of which was recently reported to be selectively elevated in cell lines expressing the Bcr-Abl tyrosine kinase. Notably, we found a strikingly low percentage of cells from the HSC/PC sub-population isolated from CML patients that were found to express the c-kit receptor (<1%) compared with the percentages of HSC/PCs expressing the c-kitR isolated from umbilical cord blood (50%) and mobilized peripheral blood (10%). Surprisingly, Tie2 receptor expression within the HSC/PC subset was extremely low from both normal and CML samples. Using in vivo transplantation studies, we provide evidence that HLA-DR, c-kitR, Tie2 and IL-3R may not be suitable markers for further partitioning of HSCs from the Lin−CD34+CD38−CD90+CD45RA− sub-population

  17. 一例A3血型样本的分子机制%Study of molecular mechanism for a blood sample with A3 phenotype

    Institute of Scientific and Technical Information of China (English)

    梁伟; 杨亮; 梅传亮; 许德义; 邓刚; 贺芸蕾; 刘奕宇; 张哲

    2015-01-01

    Objective To explore the molecular mechanism for a blood sample with mixed-field hemagglutination upon determination of ABO blood group.Methods Serological techniques were employed to identify the erythrocyte phenotype.The A and B antigens were detected by flow cytometry.The preliminary genotype of ABO gene was assayed with sequence-specific primer-polymerase chain reaction (PCR-SSP).Exons 6 and 7 of the ABO gene were amplified with PCR and analyzed by direct sequencing.Haplotypes of the ABO gene were analyzed by cloning sequencing as well.Results The serological reaction pattern has supported an O phenotype when all the tubes were centrifuged for the first time.However, a mixed-field hemagglutination of red blood cells (RBCs) with anti-A antibodies was present after the tube was centrifuged five times later.A antigens were detected on the surface of partial red blood cells of the sample by flow cytometry.PCR-SSP results have shown that the preliminary ABO genotype was A/O.Analysis of the fragments of exons 6 and 7 of the ABO gene has indicated that heterozygosis lied as follows:261G/A, 425T/T, 467C/T, 646A/T, 681A/G, 745C/T, 771C/T, 829A/G, conjecturing the genotype to be A307/O02, which was confirmed by haplotype sequence analysis.Compared with A101 allele, A307 allele has two missense mutations, 467C> T and 745C> T, which have resulted in substitutions Pro156Leu and Arg249Trp in the A glycosyltransferase polypeptide chain.Conclusion A variant allele (A307) has been identified for the first time in mainland China, which is responsible for the formation of A3 phenotype.%目的 研究1例ABO定型时出现混合外观凝集特征个体的分子遗传机制.方法 应用血清学方法和流式细胞术鉴定其ABO红细胞表型,序列特异性引物聚合酶链反应(sequence-specific primerpolymerase chain reaction,PCR-SSP)方法进行ABO基因型的初步检测,对ABO基因第6、7外显子进行聚合酶链反应和DNA序列分析,并进一步通过克隆

  18. Impact of adding nitrate or increasing the lipid content of two contrasting diets on blood methaemoglobin and performance of two breeds of finishing beef steers.

    Science.gov (United States)

    Duthie, C-A; Rooke, J A; Troy, S; Hyslop, J J; Ross, D W; Waterhouse, A; Roehe, R

    2016-05-01

    Adding nitrate to the diet or increasing the concentration of dietary lipid are effective strategies for reducing enteric methane emissions. This study investigated their effect on health and performance of finishing beef cattle. The experiment was a two×two×three factorial design comprising two breeds (CHX, crossbred Charolais; LU, Luing); two basal diets consisting of (g/kg dry matter (DM), forage to concentrate ratios) 520 : 480 (Mixed) or 84 : 916 (Concentrate); and three treatments: (i) control with rapeseed meal as the main protein source replaced with either (ii) calcium nitrate (18 g nitrate/kg diet DM) or (iii) rapeseed cake (RSC, increasing acid hydrolysed ether extract from 25 to 48 g/kg diet DM). Steers (n=84) were allocated to each of the six basal diet×treatments in equal numbers of each breed with feed offered ad libitum. Blood methaemoglobin (MetHb) concentrations (marker for nitrate poisoning) were monitored throughout the study in steers receiving nitrate. After dietary adaptation over 28 days, individual animal intake, performance and feed efficiency were recorded for a test period of 56 days. Blood MetHb concentrations were low and similar up to 14 g nitrate/kg diet DM but increased when nitrate increased to 18 g nitrate/kg diet DM (P0.05). Neither basal diet nor treatment affected carcass quality (P>0.05), but CHX steers achieved a greater killing out proportion (Pbeef steers when used within the diets studied. PMID:26627142

  19. Phosphoinositide 3-kinase γ affects LPS-induced disturbance of blood-brain barrier via lipid kinase-independent control of cAMP in microglial cells.

    Science.gov (United States)

    Frister, Adrian; Schmidt, Caroline; Schneble, Nadine; Brodhun, Michael; Gonnert, Falk A; Bauer, Michael; Hirsch, Emilio; Müller, Jörg P; Wetzker, Reinhard; Bauer, Reinhard

    2014-12-01

    The breakdown of the blood-brain barrier (BBB) is a key event in the development of sepsis-induced brain damage. BBB opening allows blood-born immune cells to enter the CNS to provoke a neuroinflammatory response. Abnormal expression and activation of matrix metalloproteinases (MMP) was shown to contribute to BBB opening. Using different mouse genotypes in a model of LPS-induced systemic inflammation, our present report reveals phosphoinositide 3-kinase γ (PI3Kγ) as a mediator of BBB deterioration and concomitant generation of MMP by microglia. Unexpectedly, microglia expressing lipid kinase-deficient mutant PI3Kγ exhibited similar MMP regulation as wild-type cells. Our data suggest kinase-independent control of cAMP phosphodiesterase activity by PI3Kγ as a crucial mediator of microglial cell activation, MMP expression and subsequent BBB deterioration. The results identify the suppressive effect of PI3Kγ on cAMP as a critical mediator of immune cell functions. PMID:25033932

  20. Effects of secoisolariciresinol diglucoside lignan-enriched flaxseed powder on body weight, visceral fat, lipid profile, adipokines, and blood pressure in rats fed a high-fructose and high-fat diet

    Science.gov (United States)

    The potential effects of secoisolariciresinol diglucoside (SDG) lignan-enriched flaxseed powder LEFP) on body weight, visceral fat, lipid profile, adipokines, and blood pressure were investigated using Sprague-Dawley rats. The animals were divided into three groups (n=8) that were fed either a norm...

  1. 瑞舒伐他汀对血脂正常的老年高血压患者血压的影响%Effect of Rosuvastatin on Blood Pressure in Senile Hypertensive Patients with Normal Blood Lipids

    Institute of Scientific and Technical Information of China (English)

    覃华东

    2013-01-01

    [Objective]To explore the effect of rosuvastatin on blood pressure in senile hypertensive pa-tients with normal blood lipids.[Methods]Totally 78 senile hypertensive patients with normal blood lipids were chosen and randomly divided into observation group and control group with 39 patients in each group. Grade III hypertension was found in 12 patients in control group and 13 patients in observation group.The control group was given nifedipine controlled-release tablets 30mg/d and hydrochlorothiazide 12.5mg/d.Based on the treatment of control group,the observation group was additionally treated with rosuvastatin calcium 10mg/d every night.The control of blood pressure and the changes of blood total cholesterol(TC),triglycer-ides(TG),low-density lipoprotein cholesterol(LDL-C),transaminase and creatine kinase(CK))in patients of two groups after 8 weeks were observed.[Results]Compared with before treatment,the levels of systolic blood pressure(SBP),diastolic blood pressure(DBP)and pulse pressure(PP)in two groups after 8 weeks of treatment were notably decreased(P 0.05).Compared with control group,the decreasing amplitude in grade III patients of observation group was more obvious(P 0.05).[Conclu-sion]Rosuvastatin is helpful for the further control of blood pressure in senile hypertensive patients with nor-mal blood lipids.The antihypertensive effect may be independent of the lipid-lowering effect.%[目的]探讨瑞舒伐他汀对血脂正常的老年高血压患者血压的影响。[方法]选取78例血脂正常的老年高血压患者随机分为对照组和观察组各39例,其中3级高血压患者分别为12例和13例。对照组予以硝苯地平控释片(30 mg/d)和氢氯噻嗪(12.5 mg/d)治疗;观察组在对照组基础上每晚加服瑞舒伐他汀钙(10 mg/d)。观察8周后两组患者血压控制情况、血清总胆固醇(TC)、甘油三酯(TG)、低密度脂蛋白胆固醇(LDL-C)、转氨酶及肌酸

  2. Effects of Cichorium intybus linn on blood glucose, lipid constituents and selected oxidative stress parameters in streptozotocin-induced diabetic rats.

    Science.gov (United States)

    Samarghandian, Saeed; Borji, Abasalt; Tabasi, Seyed Hidar

    2013-12-01

    The efficacy of herbal medicine has been confirmed in treatment of diabetes mellitus (DM) by amelioration of oxidative stress. The present study was designed to investigate protective effects of Cichorium intybus extract (CIE) against oxidative damage in diabetic rats. In this study, the rats were divided into the control (C), diabetic (D), D + CIE- treated (125 mg/kg/day) groups. Male Sprague-Dawley rats aged 9 weeks (160 ± 15 g) were administered with streptozotocin (STZ, 60 mg/kg) intraperitoneally (ip) to induce experimental diabetes. From 3 days after STZ administration to the end of the study (4 weeks) the ethanolic extract of CIE was administered (i.p) to diabetic rats. Body weight and blood glucose were measured weekly. At the end of the 4-week period, blood was drawn for biochemical assay, in order to determine the changes of cellular antioxidant defense system, serum oxidative damage and serum lipid were measured profile. CIE injection to diabetic rats resulted in significant reduction in blood glucose, triglyceride (TG), total cholesterol (TC), low density lipoprotein cholesterol (LDL-C) levels and significant elevation high density lipoprotein cholesterol (HDL-C) level as well as increase in the body weight as compared with the rats treated with STZ alone. In the treated diabetic group, we also observed the significant increase in reduced glutathione (GSH), superoxide dismutase (SOD), glutathione-S-transferase (GST) and catalase (CAT) with decline in malondialdehyde (MDA) level compared with the non-treated diabetic group. These results suggest that the Cichorium intybus extract has antioxidant properties and prevents diabetes complications by modulation of oxidative stress system. PMID:24304233

  3. 藏药察次塞曼(Ccsm)对高血脂小鼠降血脂作用的实验研究%Experimental research on the reducing blood lipid effect of the Tibetan medicine Ccsm on high blood-lipid mice

    Institute of Scientific and Technical Information of China (English)

    张莉; 刘忠

    2011-01-01

    The influence of CCSM on the reducing the blood-lipid level was analyzed on mice models with high blood lipid according to the method mentioned in the documents to analyze the level of TCG,TG and LDL-C in the serum of mice. The results showed that the med%目的:探讨Ccsm对高血脂小鼠血脂的影响。方法:按照文献方法制造高血脂小鼠模型,并检测小鼠血清总胆固醇(TCG)、甘油三酯(TG)、低密度脂蛋白(LDL—C)的水平。结果:高、中剂量组对甘油三酯(TG)有降低效粟,★p〈0.5;高、中剂量组对低密度脂蛋白(LDL-C)降低的很明显(★★p〈0.01)。对降低总胆固醇(TCG)没有明显作用。结论:Ccsm对高血脂小鼠有一定的降低血脂作用,对甘油三酯和低密度蛋白有较好的降低作用。

  4. A Metabolically Healthy Obese Phenotype in Hispanic Participants in the IRAS Family Study

    OpenAIRE

    Samaropoulos, Xanthia F.; Hairston, Kristen G.; Anderson, Andrea; Haffner, Steven M.; Lorenzo, Carlos; Montez, Maria; Norris, Jill M.; Scherzinger, Ann L.; Ida Chen, Yii-Der; Wagenknecht, Lynne E.

    2013-01-01

    Objective Some obese individuals appear to be protected from developing type 2 diabetes mellitus and cardiovascular disease (CVD). This has led to characterizing body size phenotypes based on cardiometabolic risk factors specifically as obese or overweight, and as metabolically healthy (MH) or metabolically abnormal (MA) based upon blood pressure, lipids, glucose homeostasis and inflammatory parameters. The aim of this study was to measure the prevalence of and describe fat distribution acros...

  5. Antiatherosclerotic effects of licorice extract supplementation on hypercholesterolemic patients: decreased CIMT, reduced plasma lipid levels, and decreased blood pressure

    Directory of Open Access Journals (Sweden)

    Yacov Fogelman

    2016-04-01

    Full Text Available Background: Ethanolic extract of licorice root has been shown to reduce low-density lipoprotein (LDL oxidation in atherosclerotic mice and in both hypercholesterolemic and normal lipidemic humans. Objective: This study examined the effect of licorice-root extract on carotid intima-media thickness (CIMT in individuals with hypercholesterolemia. Design: Individuals with hypercholesterolemia (total cholesterol ≥6.18 mmol/L [240 mg/dL] and without significant stenosis were randomly allocated to two groups: an experimental group that consumed 0.2 g/day of ethanolic extract of licorice root for 12 months, and a control group that received a placebo. Results: Of 110 eligible participants, 94 (41–80 years old completed the study. A significant CIMT decrease from 0.92±0.25 mm to 0.84±0.21 mm was observed in the experimental group compared with an increase from 0.85±0.17 mm to 0.88±0.19 mm in the control group. Mean plasma total cholesterol levels and LDL cholesterol decreased, at the range baseline to 1 year, from 284±32 mg/dl to 262±25 mg/dl and from 183±8.5 mg/dl to 174±9.1 mg/dl, respectively, for the experimental group (p<0.001 and from 291±35 to 289±31 mg/dl and from 177.6±10.7 to 179.3±9.6 (p=0.08, respectively, for the control group. Mean high-density lipoprotein (HDL did not change significantly in either group. In the experimental group, systolic blood pressure decreased from 138±12 mmHg to 125±13 mmHg after 1 year (p=0.01 and increased from 136±15 mmHg to 137±13 mmHg in the control group. Diastolic blood pressure decreased from 92±9 mmHg to 84±10 mmHg (p=0.01 in the experimental group and increased from 89±11 mmHg to 90±8 mmHg in the control group. Conclusion: Following 1 year of licorice consumption, mean CIMT, total cholesterol, LDL levels, and blood pressure were decreased. This suggests that licorice may attenuate the development of atherosclerosis and of related cardiovascular diseases.

  6. Lipid Peroxidation and the Total Antioxidant Status in the Pathogenesis of Age Related and Diabetic Cataracts: A Study on the Lens and Blood

    Science.gov (United States)

    Katta, Ashok V.; Katkam, R.V.; Geetha, H.

    2013-01-01

    Background: Cataract is one of the major causes of a visual impairment, which eventually leads to blindness. An oxidative damage to the lens proteins is a major factor which leads to cataract formation. Therefore, we intended to study the relationship between the biochemical markers of oxidative stress and various forms of cataracts. Methods: We examined the lenses and the sera of 120 subjects who were aged 50 to 80 years, who were distributed in two groups, viz. the study group (90 patients) and the control group (30 subjects). The oxidative stress was assessed by estimating the lipid peroxidation product in the form of thiobarbituric acid reactive substances (TBARS), the antioxidant status by measuring the levels of vitamin E and the total antioxidant capacity (TAC). The study group patients were further divided into those with nuclear cataracts (30 patients), cortical cataracts (30 patients), and diabetic cataracts (30 patients). Results: In this study, it was found that the levels of TBARS in the study group were significantly high (p<0.001), whereas the TAC (p<0.001) and the vitamin E (p<0.001) levels were significantly low, both in the lenses and the blood of the study group as compared to those of the control group. Conclusion: Thus, the present study suggests that an imbalance between the oxygen free radicals and the antioxidants may lead to lipid peroxidation in the lens. Also, the elevated levels of glucose in the diabetic cataracts lead to the auto-oxidation of glucose and a non-enzymatic glycation of the lens protein. Thereby, the high molecular weight proteins aggregate in the cataract. PMID:23905084

  7. Taurine and vitamin E supplementations have minimal effects on body composition, hepatic lipids, and blood hormone and metabolite concentrations in healthy Sprague Dawley rats

    Directory of Open Access Journals (Sweden)

    Allen PS

    2015-10-01

    Full Text Available Portia S Allen,1 Andrew W Brown,2 Michelle M Bohan Brown,3 Walter H Hsu,4 Donald C Beitz1 1Department of Animal Science, Iowa State University, Ames, IA, USA; 2Nutrition Obesity Research Center and Office of Energetics, University of Alabama at Birmingham, Birmingham, AL, USA; 3Department of Biochemistry, Biophysics, and Molecular Biology, 4Department of Biomedical Sciences, Iowa State University, Ames, IA, USA Background: As prescriptions for off-label pharmaceutical use and autonomous administration of over-the-counter nutraceuticals become mainstream, thorough assessments of these compounds are warranted. Objective: To determine the effects of gemfibrozil, rosiglitazone, metformin, taurine, and vitamin E on body composition, hepatic lipids, and metabolic hormone and blood metabolite concentrations in a healthy, outbred rat cohort. Methods: Male Sprague Dawley rats were fed a purified 10 kcal% from fat diet for 56 days and assigned to either the diet alone (control group or the diet plus oral administration of gemfibrozil (34 mg/kg, metformin (500 mg/kg, rosiglitazone (3 mg/kg, taurine (520 mg/kg, or vitamin E (200 mg/kg group. Results: Rosiglitazone administration resulted in a 56% increase in carcass adiposity, cautioning potential prescriptive off-label use. Taurine supplementation had no adverse effects on evaluated parameters. A modest but significant increase in liver triacylglycerol content was observed with vitamin E supplementation compared with control (Δ 17.2 g triacylglycerol/100 g liver lipid. Conclusion: The evaluated pharmaceuticals had effects in a healthy population similar to the reported effects in their target population, and the nutraceuticals had minimal effects on the measured physiological parameters. Keywords: thiazolidinedione, gemfibrozil, metformin, animal model

  8. Incidence and risk factors of different phenotypes of pre-diabetes in the Tehran Lipid and Glucose Study with a 9-year follow up

    Directory of Open Access Journals (Sweden)

    Hossein Ghahvehchian

    2016-03-01

    Full Text Available Background: Pre-diabetes is a high risk condition for developing type 2 diabetes mellitus and cardiovascular disease. The target of this study is evaluation of incidence rate of pre-diabetes and its risk factors in a long- term follow up in the Tehran Lipid and Glucose Study. Methods: The Tehran Lipid and Glucose Study is a prospective study being accomplished on a characteristic sample of the Tehranian residents at District 13, targeted determining the prevalence and incidence of non-communicable diseases and their risk factors. Four phases of the study have been implemented, including three-year periods from 1999 to 2011. From an overall of 12808, twenty years old and older people at baseline, after excluding participants who did not have the necessary conditions, analyses were accompanied on a 2597 male and 3282 female to estimate pre-diabetes incidence and detect their risk factors. Results: In men with incident pre-diabetes, age, fasting plasma glucose, 2-hour post-challenge plasma glucose and body mass index in addition to a positive family history of diabetes and <12 years of education were associated to a developed risk of rising pre-diabetes, but intervention and being single had a significant caring effect. In women with pre-diabetes, 2-hour post-challenge plasma glucose, triglycerides to high density lipoprotein-cholesterol ratio, waist-to-height ratio, fasting plasma glucose and a helpful family history of diabetes increased risk of pre-diabetes, however being divorced/widowed had a slighter risk. Potential risk factors for evolving combined impaired fasting glucose and impaired glucose tolerance were higher fasting plasma glucose, 2-hour post-challenge plasma glucose, body mass index and triglycerides to high density lipoprotein-cholesterol ratio, but bigger hip circumference had a caring effect. Pre-diabetes incidence rate was computed 46.1 per 1000 person-years in men and 36.8 per 1000 person-years in women. Conclusion: The study

  9. Effect of Broadcasting Gymnastics on Blood Lipid in Different Age Groups%广播体操对不同年龄人群血脂的影响

    Institute of Scientific and Technical Information of China (English)

    朱小宁; 薛芹波; 金丽

    2014-01-01

    通过问卷调查筛选出武汉市138名无遗传病史的健康中年人和非体育专业的大学生,按年龄随机分成对照组和实验组。对实验组进行为期3个月的广播体操干预,每周至少做3次,每次做6套,每套间歇1min。两组受试者在实验期间均保持正常健康饮食。受试者于实验开始前和结束后的两个早晨,在安静状态下空腹抽取6ml的静脉血,测定实验前后总胆固醇、甘油三酯、低密度脂蛋白和高密度脂蛋白的值。结果显示:3个月的广播体操能有效改善中年人的血脂水平,尤以中年男性的低密度脂蛋白含量改善效果最为明显;对青年人的血脂水平也有相应的改善效果,但相比之下较不明显。%This paper choses 138 healthy middle-aged people without genetic diseases history and non-professional sports college students in Wuhan by questionnaire,and randomly divided them into control group and experimental groups according to the age. The subjects of experimental group did broadcasting gymnastics for at least three times a week,every time do six sets,each batch for one minute. Both subjects of two groups have a normal healthy diet during the experiment. All Subjects were extracted 6ml of venous blood in the morning two days before and after they were in the experiment, in quiet state and on empty stomach,measuring their blood lipid four indicators before and after the experiment(total cholesterol,triglyceride, low density lipoprotein cholesterol and high-density lipoprotein)values. We eliminated invalid samples during the experimental process. Results showed that 3 months of broadcasting gymnastics can effectively improve middle-aged people's blood lipid levels, especially in the middle-aged men's low density lipoprotein level and it also has a comparative improvement to the youngsters' lipids level, though not so obvious contrasting with their counterpart.

  10. Cholesterol-raising diterpenes in types of coffee commonly consumed in Singapore, Indonesia and India and associations with blood lipids: A survey and cross sectional study

    Directory of Open Access Journals (Sweden)

    Buchmann Sandra

    2011-05-01

    Full Text Available Abstract Background To measure the content of cholesterol-raising diterpenes in coffee sold at the retailer level in Singapore, Indonesia and India and to determine the relationship of coffee consumption with lipid levels in a population-based study in Singapore. Methods Survey and cross-sectional study in local coffee shops in Singapore, Indonesia and India to measure the diterpene content in coffee, and a population-based study in Singapore to examine the relationship of coffee consumption and blood lipid levels. Interviews and coffee samples (n = 27 were collected from coffee shops in Singapore, Indonesia and India. In addition, 3000 men and women who were Chinese, Malay, and Indian residents of Singapore participated in a cross-sectional study. Results and Discussion The traditional 'sock' method of coffee preparation used in Singapore resulted in cafestol concentrations comparable to European paper drip filtered coffee (mean 0.09 ± SD 0.064 mg/cup. This amount would result in negligible predicted increases in serum cholesterol and triglyceride concentrations. Similarly low amounts of cafestol were found in Indian 'filter' coffee that used a metal mesh filter (0.05 ± 0.05 mg/cup. Coffee samples from Indonesia using the 'sock' method (0.85 ± 0.41 mg/cup or a metal mesh filter (0.98 mg/cup contained higher amounts of cafestol comparable to espresso coffee. Unfiltered coffee from Indonesia contained an amount of cafestol (4.43 mg/cup similar to Scandinavian boiled, Turkish and French press coffee with substantial predicted increases in serum cholesterol (0.33 mmol/l and triglycerides (0.20 mmol/l concentrations for consumption of 5 cups per day. In the Singaporean population, higher coffee consumption was not substantially associated with serum lipid concentrations after adjustment for potential confounders [LDL-cholesterol: 3.07 (95% confidence interval 2.97-3.18 for Conclusions Based on the low levels of diterpenes found in traditionally

  11. Macrophage Phenotype and Function in Different Stages of Atherosclerosis.

    Science.gov (United States)

    Tabas, Ira; Bornfeldt, Karin E

    2016-02-19

    The remarkable plasticity and plethora of biological functions performed by macrophages have enticed scientists to study these cells in relation to atherosclerosis for >50 years, and major discoveries continue to be made today. It is now understood that macrophages play important roles in all stages of atherosclerosis, from initiation of lesions and lesion expansion, to necrosis leading to rupture and the clinical manifestations of atherosclerosis, to resolution and regression of atherosclerotic lesions. Lesional macrophages are derived primarily from blood monocytes, although recent research has shown that lesional macrophage-like cells can also be derived from smooth muscle cells. Lesional macrophages take on different phenotypes depending on their environment and which intracellular signaling pathways are activated. Rather than a few distinct populations of macrophages, the phenotype of the lesional macrophage is more complex and likely changes during the different phases of atherosclerosis and with the extent of lipid and cholesterol loading, activation by a plethora of receptors, and metabolic state of the cells. These different phenotypes allow the macrophage to engulf lipids, dead cells, and other substances perceived as danger signals; efflux cholesterol to high-density lipoprotein; proliferate and migrate; undergo apoptosis and death; and secrete a large number of inflammatory and proresolving molecules. This review article, part of the Compendium on Atherosclerosis, discusses recent advances in our understanding of lesional macrophage phenotype and function in different stages of atherosclerosis. With the increasing understanding of the roles of lesional macrophages, new research areas and treatment strategies are beginning to emerge. PMID:26892964

  12. Hba1c, Blood Pressure, and Lipid Control in People with Diabetes: Japan Epidemiology Collaboration on Occupational Health Study.

    Directory of Open Access Journals (Sweden)

    Huanhuan Hu

    Full Text Available The control of blood glucose levels, blood pressure (BP, and low-density lipoprotein cholesterol (LDL-C levels reduces the risk of diabetes complications; however, data are scarce on control status of these factors among workers with diabetes. The present study aimed to estimate the prevalence of participants with diabetes who meet glycated hemoglobin (HbA1c, BP, and LDL-C recommendations, and to investigate correlates of poor glycemic control in a large working population in Japan.The Japan Epidemiology Collaboration on Occupational Health (J-ECOH Study is an ongoing cohort investigation, consisting mainly of employees in large manufacturing companies. We conducted a cross-sectional analysis of 3,070 employees with diabetes (2,854 men and 216 women aged 20-69 years who attended periodic health examinations. BP was measured and recorded using different company protocols. Risk factor targets were defined using both American Diabetes Association (ADA guidelines (HbA1c < 7.0%, BP < 140/90 mmHg, and LDL-C < 100 mg/dL and Japan Diabetes Society (JDS guidelines (HbA1c < 7.0%, BP < 130/80 mmHg, and LDL-C < 120 mg/dL. Logistic regression models were used to explore correlates of poor glycemic control (defined as HbA1c ≥ 8.0%.The percentages of participants who met ADA (and JDS targets were 44.9% (44.9% for HbA1c, 76.6% (36.3% for BP, 27.1% (56.2% for LDL-C, and 11.2% (10.8% for simultaneous control of all three risk factors. Younger age, obesity, smoking, and uncontrolled dyslipidemia were associated with poor glycemic control. The adjusted odds ratio of poor glycemic control was 0.58 (95% confidence interval, 0.46-0.73 for participants with treated but uncontrolled hypertension, and 0.47 (0.33-0.66 for participants with treated and controlled hypertension, as compared with participants without hypertension. There was no significant difference in HbA1c levels between participants with treated but uncontrolled hypertension and those with treated and

  13. The Effects of 6 Isocaloric Meals Pattern on Blood Lipid Profile, Glucose, Hemoglobin A1c, Insulin and Malondialdehyde in Type 2 Diabetic Patients: A Randomized Clinical Trial

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    Moosa Salehi

    2014-09-01

    Full Text Available Background: The present clinical trial study aims at investigating the effect of daily energy intake in 6 isocaloric meals in comparison with the current meal pattern (3 meals and 2 small snacks per day on type 2 diabetes risk markers in diabetes during 3-month period. Methods: Eighty four type 2 diabetes patients were randomly divided into 6 isocaloric meal diet or a balanced diet (3 meals and 2 snacks previous meal pattern. The planned reduced calorie diets for both groups were identical except for the meal pattern. Blood samples were analyzed before and after the investigation for fasting blood sugar (FBS, two-hour post-prandial glucose (2hPP, insulin, hemoglobin A1c (HbA1c, total cholesterol, triglyceride, HDL-C, LDL-C, and molondialdehyde (MDA concentrations. Results: HbA1c (P=0.00 and body mass index (BMI (P=0.04 values decreased significantly in the 6 isocaloric meal pattern compared with the controls. There were no significant differences in fasting serum glucose (P=0.09, insulin (P=0.65, total cholesterol (P=0.32, LDL-C (P=0.43, HDL-C (P=0.40 cholesterol, triglyceride (P=0.40, MDA (P=0.13 and 2hPP serum glucose (P=0.30 concentrations between the 6 isocaloric meal and tradition meal pattern. Conclusion: Six isocaloric meal pattern in comparison with the current meal pattern led to weight loss and improved glycemic control. Serum lipid profile and MDA did not change significantly. Trial Registration Number: IRCT201205179780N1

  14. Effect of Eicosapentaenoic Acid (EPA and Vitamin E on the Blood Levels of Inflammatory Markers, Antioxidant Enzymes, and Lipid Peroxidation in Iranian Basketball Players

    Directory of Open Access Journals (Sweden)

    R Ghiasvand

    2010-03-01

    Full Text Available "nBackground: Exercise can change the release of numerous cytokines and modulate their receptor systems. Dietary ω-3 lipids may decrease the levels of inflammatory cytokines and prostaglandins (PGs. Therefore, in this study, we investigated the effects of exercise and eicosapentaenoic acid (EPA supplementation, with or without vitamin E, on the blood levels of IL-2, TNF-α, catalase, glutathione reductase, and MDA in male basketball players."nMethods Thirty-four well-trained male basketball players were enrolled into the study. Venous blood samples were obtained from all subjects between 5:00 and 6:00 p.m., after intensive endurance exercising for 2 hours, at the baseline and after intervention. Subjects received 2g EPA and/or 400 IU vitamin E or placebo depends on their groups for 6 weeks."nResults There were significant fall (paired t-test in TNF-α in group1(P< 0.05, and in MDA in group 3 (P<0.05, whereas there were significant increase in  glutathione reductase in groups1 and 3 (P< 0.05, and in MDA in group2 (P< 0.05.There were significant differences (Tukey in glutathione reductase between groups 2 and 3 (P< 0.05, and in IL-2 between groups 1 and other groups (P< 0.01, but there were no significant differences in MDA, CAT, and TNF-α, among groups after 6 week of intervention."nConclusion: Six weeks of EPA+vitamin E supplementation enhances the plasma levels of IL-2 and erythrocytes glutathione reductase, whereas it reduces TNF-α, and 6 weeks of EPA supplementation alone enhances only the serum level of MDA.

  15. Randomized controlled trial of oatmeal consumption versus noodle consumption on blood lipids of urban Chinese adults with hypercholesterolemia

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    Zhang Jian

    2012-08-01

    Full Text Available Abstract Background Cardiovascular disease (CVD is the leading cause of death in China and worldwide. Whole grain oats can reduce risk of CVD by reducing total and LDL-cholesterol, major risk factors for CVD. While this association has been established in many populations, data from Asian populations is limited. Thus, this study investigated the impact of oat consumption on cholesterol levels in Chinese adults. Male and female data from this work were previously published separately in mandarin in two Chinese journals. The combined male and female data were reanalyzed and are presented here. Methods A randomized, controlled, parallel-arm study was conducted at Beijing Hospital, Beijing china. Subjects were adults (men and women with mild to moderate hypercholesterolemia. The oat group (n=85 consumed 100grams of instant oat cereal versus the control group (n=81 who consumed 100grams of wheat flour-based noodles daily for 6weeks. Laboratory and anthropometric measurements were conducted at baseline and at the end of the 6-week intervention. Results Dietary fiber intake increased significantly in the oat group compared to the control group at the end of the 6-week intervention. Total-, LDL-cholesterol and waist circumference decreased significantly in the oat group compared to the control. HDL-cholesterol decreased significantly in the control group versus the oat group. There were no significant changes in blood pressure, other anthropometric or laboratory measures between the two groups at the end of the intervention. Conclusions Instant oatmeal consumed daily for 6 weeks significantly increased fiber intake and decreased major risk factors for CVD in Chinese adults with hypercholesterolemia. Increased consumption of whole grains, including oats, should continue to be encouraged.

  16. Relationship between insulin resistance and blood lipid and sport in patients with type II diabetes mellitus complicated with hypertension%2型糖尿病合并高血压患者胰岛素低抗

    Institute of Scientific and Technical Information of China (English)

    谭丽艳; 杨红玉; 柴国禄; 沈凌元

    2001-01-01

    Objective To study the relationship between insulin resistance and blood pressure and blood lipid in patients with type II diabetes mellitus complicated with hypertension.Methods The serum concentration of fasting glucose,insulin,lipids and the level of blood pressure were measured in 56 patients with type II diabetes mellitus complicated with hypertension.Results The insulin sensitivity index(ISI) decreased in patients with type II diabetes mellitus complicated with hypertension compared with the patients with type II diabetes mellitus with normal blood pressure(P< 0.05).A negative correlation with hypertension was found between ISI and SBP,DBP,TG,ApoB in patients with type II diabetes mellitus complicated with hypertension(P<0.05).There was a positive correlation between ISI and HDL in patients with type II diabetes mellitus complicated with hypertension(P<0.05).Conclusion Insulin resistance presents in patients of type II diabetes mellitus complicated with hypertension.Insulin resistance is the major cause of hypertension and lipid metabolic disturbance in patients with type II diabetes mellitus complicated with hypertension.

  17. Downregulation of blood-brain barrier phenotype by proinflammatory cytokines involves NADPH oxidase-dependent ROS generation: consequences for interendothelial adherens and tight junctions.

    Directory of Open Access Journals (Sweden)

    Keith D Rochfort

    Full Text Available Blood-brain barrier (BBB dysfunction is an integral feature of neurological disorders and involves the action of multiple proinflammatory cytokines on the microvascular endothelial cells lining cerebral capillaries. There is still however, considerable ambiguity throughout the scientific literature regarding the mechanistic role(s of cytokines in this context, thereby warranting a comprehensive in vitro investigation into how different cytokines may cause dysregulation of adherens and tight junctions leading to BBB permeabilization.The present study employs human brain microvascular endothelial cells (HBMvECs to compare/contrast the effects of TNF-α and IL-6 on BBB characteristics ranging from the expression of interendothelial junction proteins (VE-cadherin, occludin and claudin-5 to endothelial monolayer permeability. The contribution of cytokine-induced NADPH oxidase activation to altered barrier phenotype was also investigated.In response to treatment with either TNF-α or IL-6 (0-100 ng/ml, 0-24 hrs, our studies consistently demonstrated significant dose- and time-dependent decreases in the expression of all interendothelial junction proteins examined, in parallel with dose- and time-dependent increases in ROS generation and HBMvEC permeability. Increased expression and co-association of gp91 and p47, pivotal NADPH oxidase subunits, was also observed in response to either cytokine. Finally, cytokine-dependent effects on junctional protein expression, ROS generation and endothelial permeability could all be attenuated to a comparable extent using a range of antioxidant strategies, which included ROS depleting agents (superoxide dismutase, catalase, N-acetylcysteine, apocynin and targeted NADPH oxidase blockade (gp91 and p47 siRNA, NSC23766.A timely and wide-ranging investigation comparing the permeabilizing actions of TNF-α and IL-6 in HBMvECs is presented, in which we demonstrate how either cytokine can similarly downregulate the

  18. 奶素食膳食对脂代谢的影响%Effect of lacto-vegetarian diet on blood lipid profile

    Institute of Scientific and Technical Information of China (English)

    李学军; 闫冰; 刘长勤; 石秀林; 王丽英; 张惠杰; 余亚信; 杨叔禹; 张素华

    2010-01-01

    Objective To study the relationship between lacto-vegetarian diet and blood lipid profile. Methods Totally 268 healthy omnivores and 388 healthy lacto-vegetarians were enrolled. The general information, smoking and drinking history, life style, and blood pressure of subjects were obtained from medical examination. Serum lipids, including total cholesterol ( TC), triglyceride ( TG), high-density lipoprotein cholesterol (HDL-C) , apo-lipoprotein A I (ApoA I ) , and apo-lipoprotein B (ApoB) , liver function, and renal function were assayed. Results Lacto-vegetarians had statistical significant lower levels of TC [ (4. 15±0. 70 vs 4.90±0.92) mmol/L,P<0.01 ], HDL-C[ (1.17±0.25 vs 1.29±0.23) mmol/L,P<0.01 ], LDL-C[ (2.43±0. 63 vs 3.04±0.82)mmol/L,P<0.01], Apo-A I [(1.06±0. 11 vs 1. 22±0.14)g/L,P<0. 01 ] and Apo-B[(0.71± 0.17 vs 0. 84±0. 20)g/L,P<0. 01 ], and also lower TC/HDL-C than omnivores. The Apo-A I/Apo-B ratio between two groups showed no difference statistically. Conclusions Chinese lacto-vegetarian diet consumed by healthy monks seems to be significantly associated with better lipid profiles.%目的 探讨奶素食膳食方式与血脂谱的关系.方法 选取268名健康的普食者和388名健康的奶素食者进行横断面研究.采集受试者的基本情况,并肘静脉采血测定空腹血糖(FBG)、总胆固醇(TC)、甘油三酯(TG)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)、载脂蛋白AI(Apo-AI)和载脂蛋白B(Apo-B),以及肝肾功等生化指标.结果 奶素食组TC[(4.15±0.70对4.90+0.92)mmol/L.P<0.01]、HDL-C[(1.17+0.25对1.29+0.23)mmol/L,P<0.01]、LDL-C[(2.43+0.63对3.04+0.82)mmol/L,P<0.01]、Apo-A I[(1.06+0.11对1.22+0.14)g/L,P<0.01]、Apo-B[(0.71±0.17对0.84+0.20)g/L,P<0.01]水平均低于普食组,TC与HDL-C的比值(3.67+0.97对3.89+0.94,P=0.019)也低于普食组.Apo-A I与Apo-B的比值两组间差异无统计学意义.结论 奶素食者具有相对良好的血脂谱特征,奶素食膳食

  19. Enhanced delivery of etoposide across the blood-brain barrier to restrain brain tumor growth using melanotransferrin antibody- and tamoxifen-conjugated solid lipid nanoparticles.

    Science.gov (United States)

    Kuo, Yung-Chih; Wang, I-Hsin

    2016-08-01

    Melanotransferrin antibody (MA) and tamoxifen (TX) were conjugated on etoposide (ETP)-entrapped solid lipid nanoparticles (ETP-SLNs) to target the blood-brain barrier (BBB) and glioblastom multiforme (GBM). MA- and TX-conjugated ETP-SLNs (MA-TX-ETP-SLNs) were used to infiltrate the BBB comprising a monolayer of human astrocyte-regulated human brain-microvascular endothelial cells (HBMECs) and to restrain the proliferation of malignant U87MG cells. TX-grafted ETP-SLNs (TX-ETP-SLNs) significantly enhanced the BBB permeability coefficient for ETP and raised the fluorescent intensity of calcein-AM when compared with ETP-SLNs. In addition, surface MA could increase the BBB permeability coefficient for ETP about twofold. The viability of HBMECs was higher than 86%, suggesting a high biocompatibility of MA-TX-ETP-SLNs. Moreover, the efficiency in antiproliferation against U87MG cells was in the order of MA-TX-ETP-SLNs  >  TX-ETP-SLNs  >  ETP-SLNs  >  SLNs. The capability of MA-TX-ETP-SLNs to target HBMECs and U87MG cells during internalization was verified by immunochemical staining of expressed melanotransferrin. MA-TX-ETP-SLNs can be a potent pharmacotherapy to deliver ETP across the BBB to GBM. PMID:26768307

  20. [The characteristics of the blood lipid composition in northern Selkups and in the immigrant population of northern Siberia and the problems of miscegenation].

    Science.gov (United States)

    Maslennikov, A B; Oteva, E A; Osipova, L P; Pikovskaia, N B; Nikolaeva, A A; Filimonova, T A; Konovalova, N A; Iagotin, V A; Bronshteĭn, E A

    1997-01-01

    Blood lipid composition was studied in 507 Selkups, native population of Tyumen Province (199 males and 308 females), 245 Slavs living in the north for more than 5 years (97 males and 148 females), 189 first generation métis born in mixed marriages (68 males and 121 females). Three age groups were analyzed: 10-19 (group 1), 20-39 (group 2), 40-59 (group 3). Male métis from groups 1, 2 and 3 had the highest levels of HDLP cholesterol, the lowest of apoB and atherogenic index. Group 2 métis were in the intermediate position between Slavs and Selkups by concentrations of total and LDLP cholesterol and triglycerides. Group 3 métis males had the lowest levels of triglycerides and intermediate levels of total and LDLP cholesterol. Group 1 and 2 métis females had the highest levels of LDLP, the lowest of apoB and low atherogenic index. In group 3, levels of total cholesterol, LDLP cholesterol, apoB and atherogenic index were as high as in non-aboriginal Slavonic population. PMID:9163044

  1. Effect of an isolated active compound (Cg-1 of Cassia glauca leaf on blood glucose, lipid profile, and atherogenic index in diabetic rats

    Directory of Open Access Journals (Sweden)

    Mazumder Papiya

    2009-01-01

    Full Text Available Objectives: The objective of present study was to evaluate the effect of active principle (Cg-1 from Cassia glauca leaf on serum glucose and lipid profile in normal and diabetic rats. Materials and Methods: Diabetes was induced by streptozotocin in neonates. Oral administration of petroleum ether, chloroform, acetone, and methanol of C. glauca leaf (100 mg/kg, p.o. for 21 days caused a decrease in fasting blood glucose (FBG in diabetic rats. Among all the extracts, acetone extract was found to lower the FBG level significantly in diabetic rats. Glibenclamide was used as standard antidiabetic drug (5 mg/kg, p.o. Acetone extract was subjected to column chromatography that led to isolation of an active principle, which was given trivial name Cg-1. Cg-1 (50 mg/kg, p.o. was studied for its hypoglycemic and hypolipidemic potential. The unpaired t-test and analysis of variance (ANOVA followed by post hoc test was used for statistical analysis. Results: Cg-1 caused a significant reduction in FBG level. It also caused reduction in cholesterol, triglycerides, and LDL levels and improvement in the atherogenic index and HDL level in diabetic rats. Conclusion: Improvement in the FBG and the atherogenic index by Cg-1 indicates that Cg-1 has cardioprotective potential along with antidiabetic activity and provides a scientific rationale for the use as an antidiabetic agent.

  2. Mycoplasma hyopneumoniae-derived lipid-associated membrane proteins induce inflammation and apoptosis in porcine peripheral blood mononuclear cells in vitro.

    Science.gov (United States)

    Bai, Fangfang; Ni, Bo; Liu, Maojun; Feng, Zhixin; Xiong, Qiyan; Shao, Guoqing

    2015-01-30

    Mycoplasma hyopneumoniae is the causative agent of swine enzootic pneumonia (EP), a disease that causes considerable economic losss in swine industry. Lipid-associated membrane proteins (LAMPs) of mycoplasma play important roles in causing mycoplasma diseases. The present study explores the pathogenic mechanisms of M. hyopneumoniae LAMPs by elucidating their role in modulating the inflammation, apoptosis, and relevant signaling pathways of peripheral blood mononuclear cells (PBMCs) of pig. LAMP treatment inhibited the growth of PBMCs. Up-regulation of cytokines, such as IL-6 and IL-1β, as well as increased production of nitric oxide (NO) and superoxide anion were all detected in the supernatant of LAMPs-treated PBMCs. Furthermore, flow cytometric analysis using dual staining with annexin-V-FITC and propidium iodide (PI) showed that LAMPs of M. hyopneumoniae induced a time-dependent apoptosis in lymphocyts and monocytes from PBMCs, which was blocked by NOS inhibitor or antioxidant. In addition, LAMPs induced the phosphorylation of p38, the ratio of pro-apoptotic Bax protein to anti-apoptotic Bcl-2, activation of caspase-3 and caspase-8, and poly ADP-ribose polymerase (PARP) cleavage in PBMCs. These findings demonstrated that M. hyopneumoniae LAMPs induced the production of proinflammatory cytokines, NO and reactive oxygen species (ROS), and apoptosis of PBMCs in vitro through p38 MAPK and Bax/Bcl-2 signaling pathways, as well as caspase activation. PMID:25481242

  3. Comparison of the effects of fish oil and olive oil on blood lipids and aortic atherosclerosis in Watanabe heritable hyperlipidaemic rabbits

    DEFF Research Database (Denmark)

    Mortensen, Alicja; Hansen, Birgit Fischer; Hansen, Jørgen Fischer; Frandsen, Henrik; Bartnikowska, Elzbieta; Andersen, Peder Søren; Bertelsen, Lone Skoubo

    1998-01-01

    To compare the effects of fish oil and olive oil on the development of atherosclerosis in Watanabe heritable hyperlipidaemic (WHHL) rabbits, 6-week-old animals were given a daily dose (1.5 ml/kg body weight) of fish oil (n 10) or olive oil (n 10) by oral administration for 16 weeks. Plasma...... treatment, and throughout the study thereafter, blood lipids were significantly (P <0.05) lower in the fish-oil group than in the olive-oil group (cholesterol: 17.0 v. 30.3 mmol/l, triacylglycerols 2.97 v. 6.25 mmol/l, at termination). In the fish-oil group cholesterol was significantly lower in...... intermediate-density lipoproteins (2.69 v. 6.76 mmol/l) and VLDL (3.36 v. 11.51 mmol/l). Triacylglycerol levels of intermediate-density lipoproteins and VLDL in the fish-oil group were also significantly lower when compared with the olive-oil group (0.54 v 1.36 mmol/l and 0.92 v. 2.87 mmol/l respectively). No...

  4. Effect of sequential heat and cold shocks on nuclear phenotypes of the blood-sucking insect, Panstrongylus megistus (Burmeister) (Hemiptera, Reduviidae)

    OpenAIRE

    Garcia Simone L; Pacheco Raquel M; Rodrigues Vera LCC; Mello Maria Luiza S.

    2002-01-01

    Thermal shocks induce changes in the nuclear phenotypes that correspond to survival (heterochromatin decondensation, nuclear fusion) or death (apoptosis, necrosis) responses in the Malpighian tubules of Panstrongylus megistus. Since thermal tolerance increased survival and molting rate in this species following sequential shocks, we investigated whether changes in nuclear phenotypes accompanied the insect survival response to sequential thermal shocks. Fifth instar nymphs were subjected to a ...

  5. The effects of continuous and intermittent aerobic exercise on lipid profile and fasting blood sugar in women with a body mass index more than 25 kg/m2: a randomized controlled trial

    Directory of Open Access Journals (Sweden)

    Alizadeh Z

    2011-07-01

    Full Text Available "n 800x600 Normal 0 false false false EN-US X-NONE AR-SA MicrosoftInternetExplorer4 /* Style Definitions */ table.MsoNormalTable {mso-style-name:"Table Normal"; mso-tstyle-rowband-size:0; mso-tstyle-colband-size:0; mso-style-noshow:yes; mso-style-priority:99; mso-style-parent:""; mso-padding-alt:0in 5.4pt 0in 5.4pt; mso-para-margin:0in; mso-para-margin-bottom:.0001pt; mso-pagination:widow-orphan; font-size:10.0pt; font-family:"Times New Roman","serif";} Background: Obesity is a major health problem all around the world. On the other hand, few people, especially women, are physically active to the levels recommended by Healthy People 2010 web site managed by the U.S. Department of Health and Human Services. The objective of this study was to compare the potential effects of intermittent and continuous exercise programs combined with concurrent calorie restriction diets on lipid profile and fasting blood sugar in overweight and obese females."n"nMethods : Forty-five women with a sedentary life style and a BMI greater than 25 kg/m2, were randomly assigned to one of the three groups (15 subjects in each group: a 40 minutes of medium-intensity intermittent exercise (64-76% of maximal heart rate, 3 bouts per day for 5 days a week, b a single bout of a 40-minute continuous exercise per day for 5 days a week, C the non-exercising control group. A self-monitored calorie restricted diet was recommended to all participants by a dietitian. The lipid profile, fasting blood sugar and blood pressure of all participants were assessed at baseline and 12 weeks after the intervention period."n"nResults : After the intervention, there were no significant differences among the groups in terms of lipid profile [cholesterol (P=0.94, triglyceride (P=0.62] fasting blood sugar (P=0.054, systolic blood pressure (P=0.84 or diastolic blood pressure (P=0.30."n"nConclusion: There seems to be no significant differences between short term continuous and intermittent aerobic

  6. 老年人运动与血脂、血糖关系的调查分析%Relationship of regular physical exercise with blood lipid and blood glucose levels in the elderly

    Institute of Scientific and Technical Information of China (English)

    白洁; 拓西平; 张文俊

    2011-01-01

    目的 探讨老年人运动与血脂、血糖的关系.方法 选择2007年4月至2009年4月来医院体检的未服用降脂药物的420名干休所老年人为研究对象,按是否规律运动分为经常运动组(n=352)及少运动组(n=68).收集两组运动情况、糖尿病患病率等相关资料,并检测两组血清中总胆同醇、甘油三酯、低密度脂蛋白胆同醇、高密度脂蛋白胆同醇、空腹血糖的水平.结果 正常运动组老年人甘油三酯、总胆固醇、高密度脂蛋白胆同醇、低密度脂蛋白胆同醇、空腹血糖均低于少运动组老年人.正常运动组老年人的糖尿病患病率明显低于少运动组老年人.结论 长期规律的运动可使老年人血脂水平向有益于健康方向变化,并降低糖尿病发生风险.%Objective To investigate the relationship of regular exercise with blood lipid and glucose levels in the elderly. Methods A total of 420 veteran cadres from Veteran Homes in Shanghai, aging from 60 to 89 years old, who visited hospital for routine physical examination from April 2007 to April 2009, were included. They were divided into frequent exercise group (n=352) and seldom exercise group (n=68) according to the exercise frequency. The information of the subjects, including exercise strength, and morbidity of type 2 diabetes mellitus (T2DM), were collected. In addition, plasma levels of triglyceride (TG), total cholesterol(TC), high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol (LDL-C) and fasting blood glucose (FBG) were examined. Results The plasma levels of TG, TC, LDL-C, HDL-C and FBG were significantly lower in frequent exercise group than in seldom exercise group. The morbidity of T2DM was lower in frequent exercise group than in seldom exercise group. Conclusion Regular physical exercise is helpful in controlling blood lipid levels, and decreasing the risk of T2DM in elderly individuals.

  7. Scientific Opinion on the substantiation of a health claim related to barley soup “Orzotto” and protection of blood lipids from oxidative damage pursuant to Article 13(5 of Regulation (EC No 1924/2006

    Directory of Open Access Journals (Sweden)

    EFSA Panel on Dietetic Products, Nutrition and Allergies (NDA

    2014-01-01

    Full Text Available Following an application from Italsur srl, submitted for authorisation of a health claim pursuant to Article 13(5 of Regulation (EC No 1924/2006 via the Competent Authority of Italy, the EFSA Panel on Dietetic Products, Nutrition and Allergies (NDA was asked to deliver an opinion on the scientific substantiation of a health claim related to barley soup “Orzotto” and protection of blood lipids from oxidative damage. The Panel considers that barley soup “Orzotto”, which is a vegetable mix containing pearled barley, Tuscan Black cabbage, potatoes, “blu savoy” cabbage, purple carrots, “bi coloured” spinach, “tri-coloured” Swiss chard, onions, red and yellow celery and parsley, is sufficiently characterised. The claimed effect, protection of blood lipids from oxidative damage, may be a beneficial physiological effect. No human intervention studies from which conclusions could be drawn for the scientific substantiation of the claim were provided by the applicant. The Panel concludes that a cause and effect relationship has not been established between the consumption of barley soup “Orzotto” and protection of blood lipids from oxidative damage.

  8. Effects of hawthorn, Semen cassiae and compatibility on blood lipid levels%山楂、决明子及其配伍的降血脂作用

    Institute of Scientific and Technical Information of China (English)

    綦振峰; 李官浩; 郑昌吉; 梁成云; 孙美玲; 杨震; 金海莉

    2013-01-01

    To study the effects of hawthorn, Semen cassiae and compatibility of hawthorn and Semen cassiae in sausage on organ coefficient and blood lipid levels of mice, fed mouse with basic feed and basic feed added with 30% ordinary sausage, sausage containing 3% hawthorn crude extract, 2% Semen cassiae crude extract, compatibility of 3% hawthorn and 2% Semen cassiae crude extract, respectively. The results showed that the sausage which was added cassia, hawthorn and their combination were all significantly reduced mice kidney index (P< 0.05), reduce the liver index (P<0.01), reduce low density lipoprotein cholestein(LDL-C) content (P<0.01), and increase high density lipoprotein cholestein (HDL-L) content(P<0.01); the sausage which was added Hawthorn can significantly reduce total cholesterol (TC) content, added combination can significantly reduce triglyceride (TG) content. But the sausage which was added hawthorn, cassia seed and its compatibility had no significant (P>0.05) effects on mice cardiac index, spleen index and abdominal fat weight. So the addition of hawthorn, cassia seed and their combination in sausage can reduce fat deposition in the liver and kidney, lower the level of blood lipid and the combination in sausage had the excellent effect.%为研究山楂、决明子及其配伍加入香肠中对小鼠脏器指数和血脂水平的影响,以小鼠为研究对象,饲喂基础饲料及分别添加了30%的普通香肠、山楂香肠(3%山楂粗提液)、决明子香肠(2%决明子粗提液)、配伍香肠(3%山楂粗提液+2%决明子粗提液)的饲料.结果表明:香肠中添加山楂、决明子及其配伍均能显著(P<0.05)降低小鼠的肾脏指数,极显著(P<0.01)降低小鼠的肝脏指数;极显著(P<0.01)降低低密度脂蛋白胆固醇(LDL-C)含量,极显著(P<0.01)升高高密度脂蛋白胆固醇(HDL-C)含量;香肠中添加山楂能显著(P<0.05)降低总胆固醇(TC)的含量,香肠

  9. Effect of the hydroalcoholic extract and juice of Prunus divaricata fruit on blood glucose and serum lipids of normal and streptozotocin-induced diabetic rats.

    Science.gov (United States)

    Minaiyan, M; Ghannadi, A; Movahedian, A; Ramezanlou, P; Osooli, F S

    2014-01-01

    Prunus divaricata (Alloocheh) is a small tree cultivating in Iran, Middle East and central Asia. Prunus genus has many species with anti-oxidant, anti-hyperlipidemia and anti-hyperglycemia effects. In the present study the anti-diabetic and anti-hyperlipidemic effects of P. divaricata fruits were examined in normal and streptozotocin (STZ)-induced diabetic rats. Both groups, control and reference rats received normal saline and glibenclamide respectively. Test groups were treated with Prunus freeze dried juice (PFDJ, 200, 400, 800 mg/kg) and Prunus freeze dried extract (PFDE, 100, 200, 400 mg/kg) started at the 3(rd) day of the experiment and continued for 27 days thereafter. Weight changes of animals were checked periodically. Fasting blood glucose (FBG) level as well as serum triglyceride (TG), total cholesterol (TC), high density lipoprotein (HDL) and low density lipoprotein (LDL) cholesterol were determined. Different treatments had no significant effect on body weight increments of normal rats, while in diabetic rats, PFDJ (800 mg/kg) and PFDE (400 mg/kg) opposed with weight loss. In acute phase of experiment (0-8 h of 3(rd) day), none of tested fractions were effective in reducing FBG and serum lipids of normal rats. During the sub-acute phase (13(th) and 30(th) days) however, the greatest test doses of PFDJ (800 mg/kg) and PFDE (400 mg/kg) induced hypoglycema. In diabetic groups, PFDJ and PFDE, at all test doses, could diminish FBG during sub-acute phase of the experiment. In addition, PFDJ and PFDE at most examined doses could diminish TG significantly and they were also effective on cholesterol derivatives in different magnitude. PMID:26339257

  10. Lipid Profile

    Science.gov (United States)

    ... be limited. Home Visit Global Sites Search Help? Lipid Profile Share this page: Was this page helpful? Also ... as: Lipid Panel; Coronary Risk Panel Formal name: Lipid Profile Related tests: Cholesterol ; HDL Cholesterol ; LDL Cholesterol ; Triglycerides ; ...

  11. Generation of mesenchymal stromal cells in the presence of platelet lysate: a phenotypic and functional comparison of umbilical cord blood- and bone marrow-derived progenitors

    OpenAIRE

    Avanzini, Maria Antonietta; Bernardo, Maria Ester; Cometa, Angela Maria; Perotti, Cesare; Zaffaroni, Nadia; Novara, Francesca; Visai, Livia; Moretta, Antonia; Del Fante, Claudia; Villa, Raffaella; Ball, Lynne M.; Fibbe, Willem E; Maccario, Rita; Locatelli, Franco

    2009-01-01

    Umbilical cord blood is an attractive source of stem cells for several cell-based therapies. In this paper, it is shown that umbilical cord blood-derived mesenchymal stroma cells, cultured in the presence of platelet lysate, have an increased proliferative potential but comparable immunomodulatory functions relative to their bone marrow-derived counterparts.

  12. Effect of sequential heat and cold shocks on nuclear phenotypes of the blood-sucking insect, Panstrongylus megistus (Burmeister (Hemiptera, Reduviidae

    Directory of Open Access Journals (Sweden)

    Garcia Simone L

    2002-01-01

    Full Text Available Thermal shocks induce changes in the nuclear phenotypes that correspond to survival (heterochromatin decondensation, nuclear fusion or death (apoptosis, necrosis responses in the Malpighian tubules of Panstrongylus megistus. Since thermal tolerance increased survival and molting rate in this species following sequential shocks, we investigated whether changes in nuclear phenotypes accompanied the insect survival response to sequential thermal shocks. Fifth instar nymphs were subjected to a single heat (35 or 40°C, 1 h or cold (5 or 0°C, 1 h shock and then subjected to a second shock for 12 h at 40 or 0°C, respectively, after 8, 18, 24 and 72 h at 28°C (control temperature. As with specimen survival, sequential heat and cold shocks induced changes in frequency of the mentioned nuclear phenotypes although their patterns differed. The heat shock tolerance involved decrease in apoptosis simultaneous to increase in cell survival responses. Sequential cold shocks did not involve cell/nuclear fusion and even elicited increase in necrosis with advancing time after shocks. The temperatures of 40 and 0ºC were more effective than the temperatures of 35 and 5ºC in eliciting the heat and cold shock tolerances, respectively, as shown by cytological analysis of the nuclear phenotypes. It is concluded that different sequential thermal shocks can trigger different mechanisms of cellular protection against stress in P. megistus, favoring the insect to adapt to various ecotopes.

  13. Effects of sericine on blood glucose and blood lipid in type 2 diabetes rats%丝胶对2型糖尿病大鼠血糖和血脂的影响

    Institute of Scientific and Technical Information of China (English)

    付秀美; 钟美蓉; 付文亮; 宋成军; 陈志宏; 薛景凤

    2011-01-01

    目的 观察丝胶对2型糖尿病大鼠血糖(BG)和血脂的影响.方法 采用腹腔注射链脲佐菌素(STZ)建立糖尿病动物模型.60只SD大鼠随机分为正常对照组、糖尿病模型组、丝胶预防组、丝胶治疗组和二甲双胍治疗组.丝胶治疗组和二甲双胍治疗组大鼠分别给予丝胶(2.4 g·kg-1·d-1)和二甲双胍(55.33 mg·kg-1·d-1)灌胃,丝胶预防组大鼠于造模前给予同等剂量丝胶灌胃.分别检测各组大鼠的BG,血甘油三酯(TG)、总胆固醇(TC)、高密度脂蛋白(HDL-C)和低密度脂蛋白(LDL-C).结果 与正常对照组相比,糖尿病模型组大鼠BG、TG、TC、LDL-C明显升高(P<0.01),HDL-C明显降低(P<0.05).与糖尿病模型组相比,丝胶治疗组、二甲双胍治疗组和丝胶预防组大鼠的BG、TG、TC和LDL-C明显降低,HDL-C明显升高(P<0.01),且丝胶治疗组与二甲双胍治疗组比较无明显差异(P>0.05).结论 丝胶对糖尿病BG升高和血脂代谢紊乱具有良好的治疗及预防作用.%Objective To observe the effects of sericine on blood glucose (BG) and blood lipid in type 2 diabetes rats. Methods The diabetes model was established by intraperitoneal injection of STZ. 60 SD rats were randomly divided into 5 groups: normal control, diabetes model, sericine treatment, sericine prevention and dimethylbiguanide treatment groups. The rats in sericine and dimethylbiguanide treatment groups were lavaged with sericine (2. 4 g · kg -1 · d- 1 ) and dimethylbiguanide (55.33 mg · kg- 1 · d - 1 ). Rats in sericine prevention group were lavaged with the same dose sericine before establishing diabetes model. BG, triglyceride (TG), total cholesterol (TC), high-density lipoprotein (HDL-C) and low-density lipoprotein (LDL-C) in serum of rats in each group were respectively detected. Results Compared with normal control group, the BG, TG, TC, LDL-C in serum of rats in model group increased obviously (P <0. 0l ), HDL-C decreased obviously (P < 0

  14. Perfil fenotípico de linfócitos periféricos de bovinos de raças européias Phenotypic profile of peripheral blood lymphocytes from European bovines

    Directory of Open Access Journals (Sweden)

    J.F.F. Bittar

    2004-02-01

    Full Text Available The phenotypic profile of bovine lymphocytes was evaluated in 18 bovines (Bos taurus from three different breeds, being nine Holstein, six Hereford, and three Brown Swiss. All animals were free from ticks and hemoparasites, as determined after jugular vein blood sampling. The immunophenotypes of peripheral lymphocytes were evaluated by flow cytometry. Peripheral lymphocytes were exposed to bovine fluorescein-labeled monoclonal antibodies including anti-CD4, anti-CD8, and anti-purified bovine CD21 specificities. After lysing the erythrocytes with a commercial lysing solution (FACS TM , the lymphocytes were washed, fixed, and evaluated by flow cytometry. Significant differences in the phenotypic profiles of peripheral lymphocytes among all breeds were found. Holstein animals showed a lower percentage of total T lymphocytes (CD4 and CD8 and higher percentage of B lymphocytes (CD21. In addition, the lymphocytes from Holstein animals showed a lower T/B ratio than the lymphocytes from Hereford animals. These results suggest the existence of different phenotypic profiles of peripheral lymphocytes from European breeds of cattle. Such differences may be related to the different pattern of immune response described for these breeds in the literature and may account to varying disease resistance among breeds.

  15. 广东省成年体检人群异常血脂水平分布情况调查%Investigation of the distribution of abnormal blood lipid levels of health examination adults of all ages in Gangdong

    Institute of Scientific and Technical Information of China (English)

    李华; 魏莲; 陈佑明; 黄敬; 刘铁连; 刘京平

    2013-01-01

    OBJECTIVE To investigate the distribution of abnormal blood lipid levels of health examination adults in Guangdong Province. METHODS We investigated and statistically analyzed abnormal blood lipid levels of 28 447 people the age of 20 years and over during period of November 2009 and November 2010 in Guangdong Province. Investigated health examination adult at different ages and gender, and abnormal blood lipid composition. According to abnormal blood lipid sample, there were three groups including only high total cholesterols (group A) ; only high triglycerides (group B) ; both high total cholesterol and triglyceride (group C). RESULTS There were significantly age differences in the proportion of abnormal blood lipid levels in the other age groups (P = 0.000) , except the abnormal blood lipid level of age group of 50- years without gender differences ; And there were significant gender differences (P ≤ 0.05) in the composition of lipid abnormalities of male and female in other ages' groups, including in group A and group B, group A and group C group B and group C, except in 20- and over 40- age groups between group B and C; And there were significant differences {P - 0.000) of the composition of lipid abnormalities on genders between three groups; also there was a different distribution of abnormal blood lipid composition of men and women of all ages. CONCLUSION There are significantly differences on the composition of lipid abnormalities, ages and genders of Guangdong adult medical health examination groups of all ages, also there are significant differences in the incidence rate of abnormal blood lipid and proportion of mixed one of female in the 50-year-old, which is highly above male with the same age, and also highly above other ages female, suggesting that postmenopausal female should pay attention to regular blood checks, for which helps prevent and reduce the incidence of cardiovascular disease.%目的 调查广东省成年体检人群各年龄段男女

  16. Association of diabetes-related distress, depression, medication adherence, and health-related quality of life with glycated hemoglobin, blood pressure, and lipids in adult patients with type 2 diabetes: a cross-sectional study

    Directory of Open Access Journals (Sweden)

    Chew BH

    2015-04-01

    Full Text Available Boon-How Chew,1 Mohd-Sidik Sherina,2 Noor-Hasliza Hassan3 1Department of Family Medicine, 2Department of Psychiatry, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, Serdang; 3Klinik Kesihatan Dengkil, Jalan Dengkil, Malaysia Abstract: This study examined the associations of diabetes-related distress (DRD, depressive symptoms, health-related quality of life (HRQoL, and medication adherence with glycemia, blood pressure (BP, and lipid biomarkers in adults with type 2 diabetes mellitus (T2D. This cross-sectional study was conducted in three Malaysian public health clinics in 2012–2013, recruited adult patients (aged ≥30 years with T2D who had been diagnosed for more than one year, were on active follow-up, and had recent blood test results. Univariable and multivariable analyses were performed to identify significant associated factors for glycated hemoglobin (HbA1c BP, and lipids. The response rate was 93.1% (700/752. The majority were females (52.8%, Malay (52.4%, and married (78.7%. DRD correlated with systolic BP (r= -0.16; depressive symptoms correlated with low-density lipoprotein cholesterol (r=0.12 and total cholesterol (r=0.13; medication adherence correlated with HbA1c (r= -0.14 and low-density lipoprotein cholesterol (r= -0.11; and HRQoL correlated with casual blood glucose (r= -0.11, high-density lipoprotein cholesterol (r= -0.13, and total cholesterol (r= -0.08. Multivariable analyses showed that HRQoL was significantly associated with casual blood glucose (adjusted B= -0.06, P=0.024; DRD was associated with systolic BP (adjusted B= -0.08, P=0.066; depressive symptoms were associated with low-density lipoprotein cholesterol (adjusted B=0.02, P=0.061, and medication adherence was associated with HbA1c (adjusted B= -0.11, P=0.082 and total cholesterol (adjusted B= -0.06, P=0.086. There were significant and distinctive associations of DRD, depressive symptoms, HRQoL, and medication adherence with

  17. Accumulation of neutral lipids in peripheral blood mononuclear cells as a distinctive trait of Alzheimer patients and asymptomatic subjects at risk of disease

    OpenAIRE

    Palmas Manuela; Zaru Cristina; Barberini Luigi; Saba Antonio; Pais Maria; Muçaka Nico; Brundu Annalisa; Angius Fabrizio; Cocco Pier; Abete Claudia; Diaz Giacomo; Mandas Antonella; Pani Alessandra; Putzu Paolo F; Mocali Alessandra

    2009-01-01

    Abstract Background Alzheimer's disease is the most common progressive neurodegenerative disease. In recent years, numerous progresses in the discovery of novel Alzheimer's disease molecular biomarkers in brain as well as in biological fluids have been made. Among them, those involving lipid metabolism are emerging as potential candidates. In particular, an accumulation of neutral lipids was recently found by us in skin fibroblasts from Alzheimer's disease patients. Therefore, with the aim to...

  18. EFFECTS OF HERBAL CAKE-SEPARATED MOXIBUSTION ON BLOOD LIPIDS,PLASMA THROMOXANE B2 AND 6-KETO-PROSTAGLANDIN F1αCONTENTS IN THE RABBIT WITH HYPERLIPEMIA

    Institute of Scientific and Technical Information of China (English)

    沈菁; 常小荣; 严洁; 曹湘萍; 岳增辉

    2004-01-01

    Objective: To observe effects of herbal cake-separated moxibustion on blood lipids, including total cholesterol (TCh), triglyceride (TG), high density lipoprotein-Ch (HDL-Ch), low density lipoprotein-Ch (LDL-Ch), apolipoprotein A (Apo A), apolipoprotein B (Apo B), and plasma thromboxane B2 (TXB2) and 6-keto-prostaglandin F1α (6-keto-PGF1α) contents and analyse its mechanism. Methods: The hyperlipemia rabbit model was established by breeding of high fat forage and injection of bovine serum albumin. They were treated respectively by continuously for 40 days. Blood TCh and TG contents were detected with enzymatic method, LDL-Ch and HDL-Ch with colorimetric method, Apo A and Apo B with electrophoretic method, and TXB2 and 6-keto-PGF1α with radioimmunoassay. Results: Both the herbal cake-separated moxibustion and direct moxibustion could effectively decrease TCh, TG, LDL-Ch, Apo B and TXB2 contents and TXB2/6-keto-PGF1α, and increase HDL-CH and 6-keto-PGF1α contents in the rabbit of hyperlipemia. Conclusion: 6-keto-PGF1α and TXB2 are possibly involved in the mechanism of herbal cake-separated moxibustion decreasing blood lipids.

  19. A rapid method for determining arachidonic:eicosapentaenoic acid ratios in whole blood lipids: correlation with erythrocyte membrane ratios and validation in a large Italian population of various ages and pathologies

    Directory of Open Access Journals (Sweden)

    Corsetto Paola

    2010-01-01

    Full Text Available Abstract Background Omega-3 and -6 polyunsaturated fatty acids (LCPUFA, are important for good health conditions. They are present in membrane phospholipids. The ratio of total n-6:n-3 LCPUFA and arachidonic acid:eicosapentaenoic acid (AA and EPA, should not exceed 5:1. Increased intake of n-6 and decreased consumption of n-3 has resulted in much higher, ca 10/15:1 ratio in RBC fatty acids with the possible appearance of a pathological "scenario". The determination of RBC phospholipid LCPUFA contents and ratios is the method of choice for assessing fatty acid status but it is labour intensive and time consuming. Aims of the study [i] To describe and validate a rapid method, suitable for large scale population studies, for total blood fatty acid assay; [ii] to verify a possible correlation between total n-6:n-3 ratio and AA:EPA ratios in RBC phospholipids and in whole-blood total lipids, [iii] to assess usefulness of these ratio as biomarkers of LCPUFA status. Methods 1 Healthy volunteers and patients with various pathologies were recruited. 2 Fatty acid analyses by GC of methyl esters from directly derivatized whole blood total lipids and from RBC phospholipids were performed on fasting blood samples from 1432 subjects categorised according to their age, sex and any existing pathologies. AA:EPA ratio and the total n-6:n-3 ratio were determined. Results AA:EPA ratio is a more sensitive and reliable index for determining changes in total blood fatty acid and it is correlated with the ratio derived from extracted RBC phospholipids. Conclusions The described AA:EPA ratio is a simple, rapid and reliable method for determining n-3 fatty acid status.

  20. TIME COURSE OF CHANGES IN BLOOD LIPID PARAMETERS IN PATIENTS WITH EARLY RHEUMATOID ARTHRITIS DURING TREAT-TO-TARGET ANTIRHEUMATIC THERAPY: ACCORDING TO 18-MONTH FOLLOW-UP FINDINGS

    Directory of Open Access Journals (Sweden)

    E. V. Udachkina

    2016-01-01

    Full Text Available The mechanisms for lowering a cardiovascular risk (CVR in patients with early rheumatoid arthritis (RA when implementing the treat-to-target strategy remain inadequately investigated.Objective: to estimate the time course of changes in blood lipid parameters in patients with early RA during Treat-totarget antirheumatic therapy at an 18-month follow-up.Subjects and methods. Seventy-four patients (73% women; median age, 56 years with early RA meeting the respective 2010 American College of Rheumatology/European League Against Rheumatism (ACR/EULAR classification criteria and moderate or high activity (median DAS28-ESR score of 5.4 were examined within the framework of the REMARCA trial. After 6-month treatment, RA activity significantly reduced (p < 0.05. At months 6 to 18, no significant change in RA activity was recorded. After 18 months, remission was observed in 31 (42% patients: in 17 (55% on methotrexate (MTX monotherapy and in 14 (45% on combined therapy with MTX and a biological agent. Blood lipid levels were determined at inclusion in the investigation, 6 and 18 months later. The values of lipid parameters were estimated in terms of the total CVR. 67.6% of the patients were classified as at very high CVR. At 18 months of treatment, 34 (46% patients were treated with statins (median atorvastatin and rosuvastatin doses were 10 mg/day each.Results and discussion. Only 12% of the patients had optimal baseline values of just all lipid parameters. The concentration of total cholesterol (TC, low-density lipoprotein cholesterol (LDL-C, and high-density lipoprotein cholesterol (HDL-C correlated negatively with C-reactive protein (CRP levels, DAS28-ESR, DAS28-CRP, and HAQ (p < 0.05. After 6-month treatment, there were increases in TC by 7%, LDL-C by 12.5%, and HDL-C by 19.7%, and a decrease in the atherogenic index by 16% (p < 0.05. ΔCRP negatively correlated with ΔTC, ΔLDL-C, and ΔHDL-C (r = -0.3; p < 0.05. A correlation of TC and LDL-C with

  1. 军队不同年龄组男性干部血脂、血压、血糖检测结果比较%Comparison on detection results of blood lipid, blood pressure and blood glucose between different age groups in cadres of the army men

    Institute of Scientific and Technical Information of China (English)

    赵建武; 严鹏飞; 许峰; 赵惠娟

    2012-01-01

    目的 比较部队男性干部不同年龄组血脂、血压、血糖检测结果,找出差异,从而有针对性的做好健康教育.方法 将2005年5月-2011年5月在我院疗养的3026例男性干部根据年龄分为:老年前期组484例,老年组1785例,高龄组757例,对各组血脂、血压、血糖异常分布及影响因素进行分析.结果 老年前期组低高密度脂蛋白、高舒张压、饮酒、吸烟、缺乏运动的检出率分别为8.7%、23.8%、47.3%、28.1%、36.8%明显高于老年组的3.4%、16.2%、35.1%、18.1%、18.2%;而高收缩压、高空腹血糖、高餐后2h血糖及超重/肥胖的检出率明显降低,差异有统计学意义(P<0.01);高龄组高收缩压、缺乏运动的检出率分别为40.2%、27.3%明显高于老年组的32.6%、18.2%,高三酰甘油、高舒张压、高餐后2h血糖,超重/肥胖,饮酒、吸烟的检查率明显降低,差异有统计学意义(P<0.01).结论 军队男性干部老年前期组低HDL-C、高DBP检出率高于老年组、高年组SBP、缺乏运动检出率明显增高;老年前组饮酒、吸烟、缺乏运动检出率增高.%Objective To compare the detection results of blood lipid, blood pressure and blood glucose between different age groups in cadres of the army men and find out the difference in order to improve the effect of health education. Methods From May 2005 — May 2011, 3 026 cases of male cadre in our hospital were selected for the study. According to the ages, patients were divided into pre-elderly group (n = 484) , old age group ( n - 1 785 ) and very old age group ( n = 757). The abnormal distributions of blood lipid, blood pressure and blood glucose and influence factors were analyzed. Results The detection rates of low high-density lipoprotein, high diastolic blood pressure, drinking, smoking, lack of exercise in pre-elderly group were 8.7% , 23. 8% , 47.3% , 28. 1% and 36. 8% respectively, significantly higher than 3. 4

  2. Defining Molecular Phenotypes of Mesenchymal and hematopoietic Stem Cells derived from Peripheral blood of Acute Lymphocytic Leukemia patients for regenerative stem cell therapy

    OpenAIRE

    Pravin D. Potdar; Rambhadur P Subedi

    2011-01-01

    Acute Lymphocytic Leukemia (ALL) is a clonal myeloid disorder affecting all age groups, characterized by accumulation of immature blast cells in bone marrow and in peripheral blood. Autologous Bone Marrow Transplantation is a present treatment for cure of ALL patients, which is very expensive, invasive process and may have possibility of transplantation of malignant stem cells to patients. In the present study, we hypothesized to isolate large number of normal Mesenchymal & Hematopoietic stem...

  3. LIPID-PROTEIN/YSTEMS OF TRANSPORTATION OF BLOOD CIOLESTEROL IN PATIENTS WITH SYSTEMIClUPUS ERYTHEMATOSUS DEPEND1N5AJN ANTIPHOSPHOLIPID SYNDROME

    Directory of Open Access Journals (Sweden)

    T. V. Popkova

    2001-10-01

    Full Text Available Objective. To stude the indices of lipid-protein blood spectrum in SLE pts with A PS as one of the risk factors of atherosclerosis development. Material and methods. 60 pts (45 females and 15 males with definite SLE (ARA criteria, 1982 were examined; age 15-44 (median age 28.9+8.1; M+G, disease duration from 2 months to 28 years (median 8.6+6.6 years APS was diagnosed in 39 out of them( G.R.V.Huges and E.N.Harris criteria, 1986 with subsequent isolation of Wr' -wKable, possible and unreliable A PS; thrombotic complications were observed in 23 out of 39 SLE pts with APS. Control group was composed of 35 practically healthy persons without risk factors of atherosclerosis and related diseases development. Results. There were no difference in the levels of cholesterol, triglycerides low density lipoproteid cholesterol and apolipoprotein В in SLE pts with APS as compared with SLE pts without APS. Concentration of high density lipoproteid cholesterol (HD LP C and apolipoprotein AI (apo A! was higher in SLE pts with APS as compared with SLE pis without APS and reached for HD LPC- 46. I±11.0mg/dl against 40.5±8.1 mg/dl, for apoAl - 133.7±28.1 tng/cll against 118.5±28.2 mg/dl correspondingly (p=0.04 and p-0.05. There was no difference in the content oJHD LP phospholipids in the group of SLE pts with and without APS. The level ofHD LP phospholipids in the group of SLE pts with APS was lower (91, 8±21.2 mg/dl as compared to the control group (131.8+3 5.1 mg/dl(p=0.001, The relation ofHD LP С/apoAl in SLE pts with APS: 0.35+0.06 against 0.38±0.08 in persons from the control group (p-0.04. With the decreased level ofHD LP in basic groups of pts as,compared with control statistically reliable difference of all HD LP phospholipids was found (p<0.001. Иге level of apoAl was lower in pts with unreliable APS as compared with such in pts with definite APS (137.4+26,6 mg/dl and 103.8±23.5 mg/dl (p-0.002. Conclusion. In SLE pts with APS there are

  4. Decrease of blood lipids induced by Shan-Zha (fruit of Crataegus pinnatifida) is mainly related to an increase of PPARα in liver of mice fed high-fat diet.

    Science.gov (United States)

    Niu, C- S; Chen, C- T; Chen, L- J; Cheng, K- C; Yeh, C- H; Cheng, J- T

    2011-08-01

    Hyperlipidemia is an important risk factor for cardiovascular diseases. Agents for the treatment of hyperlipidemia are well-developed in the clinic while PPARα is a target for lipid-lowering agents. Shan-Zha (Crataegus pinnatifida) is a traditional Chinese medicine used to increase digestion. Also, Shan-Zha fruit extract showed merit to improve obesity and hyperlipidemia in hamsters; however, the mechanism remained obscure. In the present study, hypertriglycemia and hypercholesterolemia were induced by high fat diet in C57BL/6 J male mice. Then, they were orally administered with Shan-Zha fruit extract at an effective dose of 250 mg/kg for 7 days. The liver was removed to estimate the expressions of PPARα and β-oxidation-related enzyme. Oral intake of Shan-Zha extract significantly improved hyperlipidemia in high fat diet-fed mice with an increase of PPARα expression in liver. Also, expression of PPARα-regulated β-oxidation-related enzymes was raised in liver by Shan-Zha extract. However, adipose tissue and others were not modified by this treatment of Shan-Zha fruit extract. Thus, Shan-Zha can increase the expression of PPARα to facilitate β-oxidation-related enzymes in liver for lipid degradation and blood lipid decrement. Also, this is the first report showing Shan-Zha fruit extract can influence liver to lower hyperlipidemia prior to the action in adipose tissue. PMID:21823058

  5. An assessment of morphometric indices, blood chemistry variables and an energy meter as indicators of the whole body lipid content in Micropterus dolomieu, Sander vitreus and Ictalurus punctatus

    Science.gov (United States)

    Mesa, Matthew G.; Rose, Brien P.

    2015-01-01

    The effectiveness of several non-lethal techniques as indicators of total lipid content in smallmouth bass Micropterus dolomieu, walleye Sander vitreus and channel catfish Ictalurus punctatus was investigated. The techniques included (1) the Fulton and relative condition factors, (2) relative mass, (3) plasma indicators of nutritional status (alkaline phosphatase, calcium, cholesterol, protein, triglycerides and glucose) and (4) readings from a hand-held, microwave energy meter. Although simple linear regression analysis showed that lipid content was significantly correlated with several predictor variables in each species, the r2 values for the relations ranged from 0·17 to 0·50 and no single approach was consistent for all species. Only one model, between energy-meter readings and lipid content in I. punctatus, had an r2 value (0·83) high enough to justify using it as a predictive tool. Results indicate that no single variable was an accurate and reliable indicator of whole body lipid content in these fishes, except the energy meter for I. punctatus.

  6. Pinto Bean Consumption Changes SCFA Profiles in Fecal Fermentations, Bacterial Populations of the Lower Bowel, and Lipid Profiles in Blood of Humans

    Science.gov (United States)

    Background: Beans improve serum lipids and may reduce the risk of colon cancer by increasing colonic short chain fatty acid (SCFAs) formation. Objective: We assessed whether pinto bean consumption affects 1) in vitro fecal bacterial fermentation and production of SCFAs, 2) colonic bacterial populat...

  7. Effect of Acupoint Thread Embedding on Blood Lipids and Insulin in Simple Obesity%穴位埋线对单纯性肥胖症血脂、胰岛素的影响

    Institute of Scientific and Technical Information of China (English)

    葛宝和; 王晓燕; 张彤; 安宝珍; 陈雨振; 刘颖

    2015-01-01

    目的:观察穴位埋线法治疗单纯性肥胖症的临床疗效,及其对血脂、胰岛素的影响。方法将80例患者随机分为埋线组和针刺组。穴位埋线法每星期1次,针刺法每星期6次,共治疗8星期。观察两组患者治疗前后体重、体重指数、胸围、腰围、臀围及胰岛素、血脂系列的变化情况。结果治疗后两组体重、体重指数、腰围、腰臀围比值均减少(P<0.05),空腹血清胰岛素、血脂含量降低(P<0.05),降低胰岛素抵抗(P<0.05);两组间相比无统计学差异(P>0.05)。结论穴位埋线疗法能降低单纯性肥胖症患者体重及三围,调节糖、脂代谢,实现健康减肥。%Objective To observe the clinical efficacy of acupoint thread embedding in treating simple obesity and its effect on blood lipids and insulin.Method Eighty patients were randomized into a thread embedding group and an acupuncture group. The acupoint thread embedding was given once every week, while acupuncture was given six times a week, both for 8 weeks in total. The body weight, body mass index (BMI), chest circumference, waist circumference, hip circumference, insulin and blood lipids were observed before and after intervention.Result After intervention, body weight, BMI, waist circumference, waist-hip ratio, fasting insulin level, blood lipids, and insulin resistance dropped significantly in both groups (P0.05).Conclusion Acupoint thread embedding can reduce body weight and three circumferences, and modulate glucose and lipid metabolisms, through which approaching weight loss in a healthy way.

  8. The immunological phenotype and ultrastructure of blood lymphocytes of children with the diagnosis of thyroid cancer who lived near the Chernobyl NPS

    International Nuclear Information System (INIS)

    On the flow cytometric and electron microscopic study of blood cells in children with papillary thyroid cancer, who lived near the Chernobyl Nuclear Power Station, we revealed a decrease in the number and changes in the ultrastructure of T-lymphocytes (CD3+ cells), T-helpers (CD4+ cells), and NK cells (CD56+cells) as well as eosinophilia. We suggest that the impairment of T-cellular and natural immunity may be a promoting factor of the malignant transformation of thyrocytes induced by radioiodine

  9. 低氧和运动对营养性肥胖大鼠血脂代谢的影响%Effect of Hypoxia and Exercise on Blood Lipid Metabolism of Diet-induced Obese Rats

    Institute of Scientific and Technical Information of China (English)

    邱烈峰

    2013-01-01

      采用高脂饲料饲喂健康 SPF 级雄性 SD 大鼠建立营养性肥胖模型,对肥胖大鼠进行4周的低氧和/或运动干预。结果表明,低氧、运动以及低氧复合运动均可改善营养性肥胖大鼠血脂代谢。其中低氧复合运动改善血脂代谢效果优于单纯低氧和单纯运动。%To investigate the effect of hypoxia and /or exercise intervention on blood lipid metabolism of diet -in-duced obese rats, SPF-grade healthly male Sprague-Dawley rats were fed high fat forage to establish the obese model . Then the obese model were subjected to hypoxia exposure in normobaric hypoxic tent simulating and /or exercise on a motor-driven rodent treadmill for 4 weeks.Results showed that the combined effects of hypoxia and exercise is more ef -fective than only hypoxia or only exercise in improving blood lipid metabolism in diet -induced obese rats.

  10. The Influence of Insecticide Resistance, Age, Sex, and Blood Feeding Frequency on Thermal Tolerance of Wild and Laboratory Phenotypes of Anopheles funestus (Diptera: Culicidae).

    Science.gov (United States)

    Lyons, C L; Oliver, S V; Hunt, R H; Coetzee, M

    2016-03-01

    Resistance to insecticides is a global phenomenon and is increasing at an unprecedented rate. How resistant and susceptible strains of malaria vectors might differ in terms of life history and basic biology is often overlooked, despite the potential importance of such information in light of changing climates. Here, we investigated the upper thermal limits (ULT50) of wild and laboratory strains of Anopheles funestus Giles mosquitoes, including resistance status, sex, age, and blood feeding status as potential factors influencing ULT50. No significant differences in ULT50 were observed between strains displaying different resistance patterns, nor was there a significant difference between wild and laboratory strains. In some instances, strains showed a senescence response, displaying decreased ULT50 with an increase in age, and differences between males and females (females displaying higher ULT50 than males). Blood feeding did not seem to influence ULT50 in any way. For An. funestus, it seems evident that there is no cost to resistance despite what is displayed in other anopheline species. This could have significant impacts for vector control, with resistant populations of An. funestus performing just as well, if not better, than susceptible strains, especially under changing environmental conditions such as those expected to occur with climate change. PMID:26718714

  11. 精神分裂症患者血糖、血脂及血流变联合测定临床意义%Clinical significance of combined detection of glucose,blood lipids and hemorheology in patients with schizophrenia

    Institute of Scientific and Technical Information of China (English)

    何玉琪; 潘名志

    2012-01-01

    Objective To investigate the clinical significance of glucose, blood lipids and hemorheology in patients with schizophrenia. Methods 60 patients with schizophrenia and 40 healthy persons were enrolled. Blood glucose, blood lipids and hemor heology were detected separately and results were compared. Results Levels of blood glucose, blood lipids, hematocrit and hemorheology in patients with schizophrenia were significantly higher than those in healthy control group(P<0. 01 or P<0. 05). Conclusion Levels of blood glucose,blood lipids and hemorheology in patients with schizophrenia might be abnormal. It could have important clinical significance in the diagnosis and treatment of schizophrenia to quantitatively analyze the levels of blood glucose, blood lipids and hemorheology.%目的 探讨精神分裂症患者血糖、血脂及血液流变学的变化及临床意义.方法 收集60例精神分裂症患者和40例健康人,分别对两组对象进行血糖、血脂及血液流变学的检测.并对两组的结果进行比较.结果 精神分裂症患者血糖、胆固醇、三酰甘油、红细胞比容及全血黏度显著高于对照组(P<0.05).结论 精神分裂症患者的体内血糖、血脂及血液流变学均有不同程度的改变,血糖、血脂及血液流变学水平测定在精神分裂症临床治疗中有着重要意义.

  12. Association of diabetes-related distress, depression, medication adherence, and health-related quality of life with glycated hemoglobin, blood pressure, and lipids in adult patients with type 2 diabetes: a cross-sectional study.

    Science.gov (United States)

    Chew, Boon-How; Sherina, Mohd-Sidik; Hassan, Noor-Hasliza

    2015-01-01

    This study examined the associations of diabetes-related distress (DRD), depressive symptoms, health-related quality of life (HRQoL), and medication adherence with glycemia, blood pressure (BP), and lipid biomarkers in adults with type 2 diabetes mellitus (T2D). This cross-sectional study was conducted in three Malaysian public health clinics in 2012-2013, recruited adult patients (aged ≥30 years) with T2D who had been diagnosed for more than one year, were on active follow-up, and had recent blood test results. Univariable and multivariable analyses were performed to identify significant associated factors for glycated hemoglobin (HbA1c) BP, and lipids. The response rate was 93.1% (700/752). The majority were females (52.8%), Malay (52.4%), and married (78.7%). DRD correlated with systolic BP (r= -0.16); depressive symptoms correlated with low-density lipoprotein cholesterol (r=0.12) and total cholesterol (r=0.13); medication adherence correlated with HbA1c (r= -0.14) and low-density lipoprotein cholesterol (r= -0.11); and HRQoL correlated with casual blood glucose (r= -0.11), high-density lipoprotein cholesterol (r= -0.13), and total cholesterol (r= -0.08). Multivariable analyses showed that HRQoL was significantly associated with casual blood glucose (adjusted B= -0.06, P=0.024); DRD was associated with systolic BP (adjusted B= -0.08, P=0.066); depressive symptoms were associated with low-density lipoprotein cholesterol (adjusted B=0.02, P=0.061), and medication adherence was associated with HbA1c (adjusted B= -0.11, P=0.082) and total cholesterol (adjusted B= -0.06, P=0.086). There were significant and distinctive associations of DRD, depressive symptoms, HRQoL, and medication adherence with glycemia, BP, and lipid biomarkers. Unexpected beneficial therapeutic effects of DRD on BP require further study. A multidisciplinary approach may be needed for risk management in adults with T2D at the primary care level. PMID:25995640

  13. Glycaemic index and glycaemic load in relation to blood lipids - 6 years of follow-up in adult Danish men and women

    DEFF Research Database (Denmark)

    Oxlund, Anne Lene; Heitmann, Berit Lilienthal

    2006-01-01

    : Prospective study with 6 years of follow-up. Overall dietary GI and GL of each participant were assessed from diet history interviews. SETTING: Population-based study. Subjects Three hundred and thirty-five healthy men and women aged 35-65 years selected randomly from a larger sample of Danish adults. RESULTS......BACKGROUND: Cross-sectional studies have suggested an association between glycaemic index (GI) or glycaemic load (GL) and serum lipids. However, no prospective studies have been performed. OBJECTIVE: To examine whether GI or GL was associated with subsequent changes in serum lipids. DESIGN......: In men GI was directly related to changes in total cholesterol (DeltaTC), regression coefficient (beta) = 0.0044 (95% confidence interval (CI): 0.0008-0.0081) and GL was positively related to changes in low-density lipoprotein cholesterol (DeltaLDL), beta = 0.1554 (95% CI: 0.0127-0.2982). Furthermore...

  14. Blood lipid levels in relation to glucose status in European men and women without a prior history of diabetes: the DECODE Study

    DEFF Research Database (Denmark)

    Zhang, L.; Qiao, Q.; Tuomilehto, J.;

    2008-01-01

    OBJECTIVE: Dyslipidaemia is present not only in diabetic but also in prediabetic subjects. The purpose of this study is to investigate the relationship between lipid and glucose levels in a large European population without a prior history of diabetes. RESEARCH DESIGN AND METHODS: Data from...... the population-based studies of 8960 men and 10,516 women aged 35-74 years representing 15 cohorts in 8 European countries were jointly analyzed. Multivariate adjusted linear regression analyses with standardized coefficients (beta) were performed to estimate the relationship between lipid and plasma glucose....... RESULTS: In subjects without a prior history of diabetes, positive relationships were shown between fasting plasma glucose (FPG) and total cholesterol (TC) (beta=0.06 and 0.03, respectively for men and women, p

  15. Combined Effect of heat stress and radiation exposure on levels of testosterone lactate dehydrogenase, total lipids and cholesterol in blood plasma and testicular tissue of male albino rats

    International Nuclear Information System (INIS)

    Hyperthermia and radiotherapy are used as relatively successful modalities in the treatment of some diseases. This work aims to gain a better understanding about the testicular physiological changes caused by a combined stress of heat and gamma radiation. Rats were divided into four groups : 1.Control, 2.Irradiated at a dose level 6 Gy, 3.Exposed to heat tress for one, 3, 5 and 7 days and group 4 exposed to heat tress as group 3 followed by irradiation directly at the end of heat treatment. Plasma levels of testosterone, lactate dehydrogenase (LDH), total lipids and total cholesterol, as well as, testicular content of total lipids and total cholesterol were detected. Results showed that irradiation (6 Gy) induced significant decreases (P<0.001) in plasma testosterone level, whereas, it elevated significantly the plasma levels of LDH activity and total cholesterol. The heat stress caused significant declines in plasma testosterone , LDH (after 1, 3 and 5 days) and total lipids, but not testicular content of total lipids. Conversely, it increased the rectal temperature and both, plasma and testicular tissue content of total cholesterol levels. The whole-body gamma irradiation direcrly post heat stress intervals boosted the heat stress effect on all the examined parameters. Hence it could be concluded that exposure to whole-body gamma irradiation in high ambient temperatures diminished the testicular function by decreasing the sexual hormone testosterone although its precursor cholesterol increased in both plasma and testicular tissue. This combined treatment seemed to block the mechanism of testosterone formation in testicular tissue, and in turn, decresed its levels in plasma. Further investigations are needed to study such combined effect of heat stress andirradiation on the testicular potency and the testicular function

  16. Lipid Micro Emboli in Cardiac Surgery

    OpenAIRE

    Eyjolfsson, Atli

    2011-01-01

    This thesis is based on five studies. It includes the characterization of lipid micro emboli in shed mediastinal blood during cardiac surgery. The reuse of blood directly by cardiotomy suction is a common practice in order to reduce the need of blood transfusions. In shed mediastinal blood we showed that there is an abundance of lipid particles in the size range of 10-60μm. These lipid particles are also recirculated by the cardiotomy suction, through filters and the plastic tubing in the car...

  17. Metabonomic phenotype and identification of "heart blood stasis obstruction pattern" and "qi and yin deficiency pattern" of myocardial ischemia rat models

    Institute of Scientific and Technical Information of China (English)

    YAN Bei; GU ShengHua; HUANG Qing; ZHENG YuanTing; A JiYe; HAO HaiPing; WANG GuangJi; ZHU XuanXuan; ZHA WeiBin; LIU LinSheng; GUAN EnZe; ZHANG Ying

    2009-01-01

    The traditional Chinese medicine concepts of "Xinxueyuzuzheng (heart blood stasis obstruction pattern)" and "Qiyinliangxuzheng (qi and yin deficiency pattern)" for myocardial ischemia rat models were constructed in the present study.Endogenous metabolites in rat plasma were analyzed using the GC/TOF-MS-based metabonomic method.Significant metabolic differences were observed between the control and two model groups,and the three groups were distinguished clearly by pattern recognition.Compared with those of the control,the levels of hydroxyproline,threonic acid,glutamine and citric acid were strikingly up-or down-regulated in model rats.The metabolites contributing most to the classification between the two "pattern" rats were identified,such as valine,serine,threonine,ornithine,hydroxyproline,lysine,2-hydroxybutanoic acid,3.hydroxybutanoic acid,galactofuranose and inositol.These compounds were indicated as the potential biomarkers.The results suggested that the two "patterns" are involved in dysfunction in oxidative stress,energy metabolism and amino acid metabolism.These findings also provided the substantial foundation for exploring the scientific connotation of these two "Zhengxing (pattern types)" of myocardial ischemia,and "Bianzheng (pattern identification)".

  18. Metabonomic phenotype and identification of “heart blood stasis obstruction pattern” and “qi and yin deficiency pattern” of myocardial ischemia rat models

    Institute of Scientific and Technical Information of China (English)

    2009-01-01

    The traditional Chinese medicine concepts of "Xinxueyuzuzheng (heart blood stasis obstruction pattern)" and "Qiyinliangxuzheng (qi and yin deficiency pattern)" for myocardial ischemia rat models were constructed in the present study. Endogenous metabolites in rat plasma were analyzed using the GC/TOF-MS-based metabonomic method. Significant metabolic differences were observed between the control and two model groups, and the three groups were distinguished clearly by pattern recognition. Compared with those of the control, the levels of hydroxyproline, threonic acid, glutamine and citric acid were strikingly up or down-regulated in model rats. The metabolites contributing most to the classification between the two "pattern" rats were identified, such as valine, serine, threonine, ornithine, hydroxyproline, lysine, 2-hydroxybutanoic acid, 3-hydroxybutanoic acid, galactofuranose and inositol. These compounds were indicated as the potential biomarkers. The results suggested that the two "patterns" are involved in dysfunction in oxidative stress, energy metabolism and amino acid metabolism. These findings also provided the substantial foundation for exploring the scientific connotation of these two "Zhengxing (pattern types)" of myocardial ischemia, and "Bianzheng (pattern identification)".

  19. 吸烟对2型糖尿病男性患者血脂水平的影响%Effect of smoking on the blood lipid level of male patients with type 2 diabetes

    Institute of Scientific and Technical Information of China (English)

    刘佳; 王佑民; 冯双双

    2015-01-01

    目的:探讨吸烟对2型糖尿病男性患者三酰甘油( TG)、胆固醇( TC)、高密度脂蛋白( HDL-C)、低密度脂蛋白( LDL-C)的影响。方法选取2型糖尿病男性患者144例,按吸烟情况分为不吸烟组(从不吸烟)、戒烟组(戒烟半年以上)、少量吸烟组(≤20支/d)、大量吸烟组(>20支/d),采用现场调查结合病例对照研究的方法,问卷调查表的形式,询问患者年龄、糖尿病病程、运动、饮食、吸烟、调脂药的应用等情况;测量血压、身高、体重、腰围、臀围,计算体重指数( BMI)、腰臀比( WHR)、体脂分数等指标。实验室检测TG、TC、HDL-C、LDL-C等,比较各组间血脂、载脂蛋白水平的差异;采用协方差分析校正其他影响因素,比较各组血脂的差异情况;逐步回归及偏相关分析,了解吸烟对血脂影响的相关性及影响程度。结果①不吸烟组与大量吸烟组比较, TG显著降低( P 20 per day) smokers. All of them were investigated about the age, diabetes pathogenesis, exercise, diet, smoking, lipid-lowering drugs used and checked with TG, TC, HDL-C, LDL-C and so on. The difference of TG, TC, HDL-C, LDL-C among these four groups was compared by the methods of covariance analysis, regression analysis and partial correlation to evalu-ate the relationships of smoking with blood lipid. Results TG of heavy smokers was higher than the non-smokers and HDL-C was lower after correcting the effects of other possibly relative factors(P<0. 05). HDL-C of the former smokers was higher than smokers and TG was lower than heavy smokers(P<0. 05). Smoking was an independent risk factor for HDL-C ( P<0. 05 ,β= -0. 213 ) , and there was a significantly negative relationship between daily cigarette consumption and HDL-C ( r= -0. 223 , P<0. 05 ) . Conclusion Smoking is an important risk factor for the diabetic male patients’ metabolism of the TG and HDL-C,independent of age, body mass index, blood pres

  20. Impact of SDG on blood glucose and blood lipid concentrates of middle and old aged female patients%亚麻木脂素对中老年妇女血糖和血脂浓度影响的研究

    Institute of Scientific and Technical Information of China (English)

    毛艺; 胡晓军

    2011-01-01

    目的 探讨膳食亚麻木脂素(SDG)对中老年妇女血糖和血脂浓度的影响.方法 采用随机数字表法将24例高血糖患者分为4组各6例,食用从亚麻籽提取的木脂素(SDG),低剂量组200 mgSDG/d、中剂量组400 mg SDG/d、高剂量组600 mg SDG/d、对照组服用安慰剂,观察SDG对血糖和血脂的影响.结果 与对照组比较,中剂量组服用8周或高剂量组服用SDG4周,血清葡萄糖(GLU)浓度显著降低(t值分别为5.7021、6.9241,P均<0.05).结论 服用400 mg/d的SDG 8周或600 mg/d的SDG4周,可降低血清葡萄糖GLU浓度.%Objective To investigate the influence of SDG on blood glucose and blood lipid concentrates of middle and old aged patients. Methods The impacts of SDG on blood glucose and blood lipid concentrates in body of patients with hyperglycemia were conducted through an 8-week random and double-blind experiments with controlled group only given placebo. The influence degree of SDG on blood glucose and blood lipid concentrates of the selected patients was analyzed. Results The results showed that serum glucose of the groups received 400mg/d of dietary SDG for 8 weeks or 600 mg/d for 4 weeks decreased significantly compared with the controlled group (P<0.05). Conclusion Giving either 400 mg/d of dietary SDG for 8 weeks or 600 mg/d for 4 weeks to patients could significantly decrease the serum glucose concentrates of them.

  1. 正常人外周血Th17细胞的频率、表型及其功能特征%Frequency, phenotype and functional characterization of peripheral blood Th17 cells in healthy individual

    Institute of Scientific and Technical Information of China (English)

    周茂华; 张敏; 欧阳维富; 吴璐思

    2013-01-01

    目的:研究正常人外周血Th17细胞的频率、表型及其功能特征.方法:分离正常人外周血单个核细胞(PBMCs),加入刺激剂PMA和离子霉素,同时加入蛋白转运抑制剂BFA,细胞置于37℃、50 mL/LCO2培养箱培养4~5h.收集细胞,以荧光素-mAb标记后,用流式细胞仪检测.结果:正常人PBMCs经PMA+ Ionomycin刺激后,Th17细胞的频率为(1.63±0.72)%,细胞表达CD45RO (99.32±0.79)%、CCR6(97.37±3.04)%和CCR4 (96.27±3.49)%;约15%的Th17细胞表达IFN-γ,超过80%的Th17细胞表达IL-2,但Th17细胞几乎不表达IL-10或IL-4.结论:正常人外周血中存在记忆性Th17细胞,该细胞表达趋化因子受体CCR6和CCR4,且具有不同于Th1和Th2细胞的功能特征.%Objective To analyze the frequency,phenotype and functional characterization of peripheral blood Th17 cells in healthy individuals.Methods PBMCs were isolated from normal health human peripheral blood and cultured with PMA and ionomycin in the presence of Brefeldin A for 4 ~ 5 h at 37 ℃ in a 5% CO2humidified atmosphere.The frequency of subpopulation of IL-17-producing cells,the expression of IL-17 with Th1 and Th2 cytokines and the phenotypic characterization of IL-17-producing cells were assessed by flow cytometry.Results The frequency of Th17 cells was (1.63 ± 0.72)% following stimulation with PMA plus ionomycin.Th17 cells express CD45RO,CCR6 and CCR4.About fifteen percentage of Th17 cells showed a double IL-17+ IFN-γ+ phenotype,more than eighty percentage of Th17 cells showed a double IL-17+IL-2+phenotype,whereas cells producing both IL-17 and Th2 cytokines were almost undetectable.Conclusion Memory Th17 cells with the expression of CCR6 and CCR4 are apparent in healthy individual PBMCs and Th17 cells are different from Th1/Th2 cells.

  2. Effect of blood lipid on the change of brachial-ankle pulse wave velocity among prehypertensive population%血脂水平对血压正常高值人群脉搏波传导速度的影响

    Institute of Scientific and Technical Information of China (English)

    王林; 帅平; 刘玉萍; 程幼夫; 杨华; 李婷欣; 龚立荣; 任姣姣; 王洪佳

    2014-01-01

    Objective To explore the effect of blood lipid and lipoprotein ratios on the change of brachial-ankle pulse wave velocity (baPWV) among prehypertensive subjects.Methods 11 611 subjects with normal blood pressure (BP) were divided into two groups,which was one with optimal blood pressure (B P < 120/80 mmHg) and the other with prehypertension (BP:120-139/80-89 mmHg).Height,weight,baPWV,fasting blood-glucose,TC,TG,LDL-C and HDL-C were detected.Results The abnormal rate ofbaPWV in prehypertension group was obviously higher than that in the optimal blood pressure group.For optimal blood pressure group,the abnormality of TG,TC,LDL-C,TC/HDL-C as well as LDL-C/HDL-C,caused the increase of baPWV significantly (P<0.001).For prehypertensive group,the abnormality of TC and LDL-C caused the significant increase of baPWV (P<0.001).Results from logistic regression analysis showed that except for age,BMI and fasting blood-glucose,TC/HDL-C increasing was the independent risk factor in optimal blood pressure group,while TG increasing was for the prehypertension group.Conclusion With different normal BP level,both abnormality of blood lipid and lipoprotein ratio were the independent risk factors for baPWV increasing.%目的 探讨血脂和脂蛋白比值对血压正常高值人群脉搏波传导速度的影响.方法 选择11 611名血压正常的健康体检者,分为正常血压值组(血压< 120/80 mmHg)和血压正常高值组(血压为120 ~ 139/80 ~ 89 mmHg).应用全自动动脉硬化仪测定人选者肱踝脉搏波传导速度(baPWV),同时测量身高、体重、FPG、TC、TG、HDL-C、LDL-C等指标,并计算HDL-C和TC/HDL-C比值、LDL-C/HDL-C比值.分析血脂和脂蛋白比值异常情况对不同血压组脉搏波传导速度的影响.结果 血压正常高值组的baPWV异常率均高于血压正常组.血压正常组中除HDL-C外,TC、TG、LDL-C、TC/HDL-C、LDL-C/HDL-C的升高均使baPWV的异常率显著增加(P<0.001).血压正常高值组中,TC

  3. 非酒精性脂肪肝患者铜营养状况及其与血脂的关系%Observational Research on Copper Nutrition and Blood Lipid among Patients with Nonalcoholic Fatty Liver Disease

    Institute of Scientific and Technical Information of China (English)

    王绩凯; 冯哲伟; 李青艳; 李迎春

    2011-01-01

    Objective To evaluate the levels of copper nutrition and the relationship with lipid in patients with nonalcoholic fatty liver disease (NAFLD). Methods 1:1 matched case-control study was conducted among 150 patients with NAFLD and 150 healthy controls. A self-designed questionnaire was used to collect their general information and intake frequency of 4 kinds of food that was rich in copper. Serum copper and blood lipid were detected and then compared by t-test, and correlation analysis was used to explore the blood lipid' s influence on serum copper. Results There was no statistical difference in the intake frequency of 4 kinds of copper-rich food between the two groups. Serum copper of NAFLD patients (1.07±0.25) mg/L was significantly higher than that of healthy controls (1.01±0.22) mg/L (t=3.12, P=0.002). Compared with the controls, the levels of TC, TG and LDL-C were higher, but the level of HDL-C was lower in patients. Correlation analysis revealed that serum copper was significant positive correlated with TC and LDL-C, and was significant negative correlated with HDL-C. Conclusion Serum copper and lipid metabolism disorder exist in NAFLD patients, and serum copper relates with blood lipid metabolism disorder, but doesn't relate with the intake of 4 kinds of copper-rich food.%目的 了解非酒精性脂肪肝( NAFLD)患者铜营养代谢水平及其与血脂的关系,为NAFLD的防治提供科学依据.方法 采用1∶1配对病例-对照研究方法,自行设计调查问卷收集150例非酒精性脂肪肝患者和150例正常对照的基本信息及四种含铜丰富食品的摄入频率,并检测其血清铜含量及血脂各指标,对血铜与血脂等指标进行线性相关分析.结果 非酒精性脂肪肝组四种含铜丰富食品的摄入频率和对照组间差异无显著性,但血清铜(1.07-0.25)mg/L高于对照组(1.01±0.22) mg/L,差异有统计学意义(t=3.12,P=0.002).非酒精性脂肪肝患者总胆固醇(TC)、三酰甘油(TG)

  4. Blood pressure and fasting lipid changes after 24 weeks’ treatment with vildagliptin: a pooled analysis in >2,000 previously drug-naïve patients with type 2 diabetes mellitus

    Science.gov (United States)

    Evans, Marc; Schweizer, Anja; Foley, James E

    2016-01-01

    Introduction We have previously shown modest weight loss with vildagliptin treatment. Since body weight balance is associated with changes in blood pressure (BP) and fasting lipids, we have assessed these parameters following vildagliptin treatment. Methods Data were pooled from all double-blind, randomized, controlled, vildagliptin mono-therapy trials on previously drug-naïve patients with type 2 diabetes mellitus who received vildagliptin 50 mg once daily (qd) or twice daily (bid; n=2,108) and wherein BP and fasting lipid data were obtained. Results Data from patients receiving vildagliptin 50 mg qd or bid showed reductions from baseline to week 24 in systolic BP (from 132.5±0.32 to 129.8±0.34 mmHg; P<0.0001), diastolic BP (from 81.2±0.18 to 79.6±0.19 mmHg; P<0.0001), fasting triglycerides (from 2.00±0.02 to 1.80±0.02 mmol/L; P<0.0001), very low density lipoprotein cholesterol (from 0.90±0.01 to 0.83±0.01 mmol/L; P<0.0001), and low density lipoprotein cholesterol (from 3.17±0.02 to 3.04±0.02 mmol/L; P<0.0001), whereas high density lipoprotein cholesterol increased (from 1.19±0.01 to 1.22±0.01 mmol/L; P<0.001). Weight decreased by 0.48±0.08 kg (P<0.001). Conclusion This large pooled analysis demonstrated that vildagliptin shows a significant reduction in BP and a favorable fasting lipid profile that are associated with modest weight loss. PMID:27574437

  5. Milk lipids

    Science.gov (United States)

    Milk fat conveys a number of desirable qualities to food, and various lipid components contribute to human nutrition and health. Over 96% of milk lipids consist of triacylglycerols, which contain a variety of fatty acids. Di- and monoacylglycerols, free fatty acids, sterols, and phospho-, glyco-,...

  6. Relative and cumulative effects of lipid and blood pressure control in the Stroke Prevention by Aggressive Reduction in Cholesterol Levels trial

    DEFF Research Database (Denmark)

    Amarenco, Pierre; Goldstein, Larry B; Messig, Michael;

    2009-01-01

    BACKGROUND AND PURPOSE: The relative contributions of on-treatment low- and high-density lipoprotein cholesterol (LDL-C, HDL-C), triglycerides, and blood pressure (BP) control on the risk of recurrent stroke or major cardiovascular events in patients with stroke is not well defined. METHODS: We...

  7. 吸烟对男性糖尿病患者血脂代谢的影响%Effects of smoking on blood lipid metabolism in male patients with diabetes mellitus

    Institute of Scientific and Technical Information of China (English)

    吴让兵; 杜小梅; 王华; 何劲松; 李明锐; 李月姗; 王莉莉

    2016-01-01

    Objective To explore the effects of smoking on blood lipid metabolism in male patients with diabe-tes mellitus. Methods A total of 156 patients with diabetes mellitus were enrolled in the study, which were divided into no-smoking group, stop-smoking group, slight smoking group and heavy smoking group according to smoking status. The history of diabetes, smoking status (including time of smoking, daily amount of smoking, smoking cessation age), di-et and exercise situation (daily exercise time and amount of exercise) were asked. The height, weight, waist circumfer-ence, hip circumference and body fat percentage were measured. The levels of fasting blood glucose (FBG), postprandial 2 hours blood glucose (2 hPBG) and triglyceride (TG), total cholesterol (TC), high density lipoprotein-cholesterol (HDL-C) and low density lipoprotein-cholesterol (LDL-C) were detected. The effect of smoking on blood lipids was ana-lyzed by covariance analysis and multiple stepwise regression analysis. Results Compared with no-smoking group, the level of TG was significantly higher in heavy smoking group (P0.05). Compared with stop-smoking group, the level of HDL-C in slight smoking group was significantly lower (P0.05);与戒烟组比较,少量组HDL-C显著较低(P<0.05),大量组TG水平较高而HDL-C水平较低(P<0.05);协方差分析表明,大量组TG和HDL-C水平与戒烟组比较差异均具有统计学意义(P<0.05).吸烟与HDL-C间呈显著负相关性(P<0.05).结论 吸烟是男性糖尿病患者体内脂代谢紊乱的独立危险因素,戒烟对调节脂代谢具有重要意义.

  8. 沂州木瓜汁抗氧化、降血脂保健功能的研究%Study on the health function on antioxidation and reducing blood lipid of Yizhou Chaenomeles fruit juice

    Institute of Scientific and Technical Information of China (English)

    魏东

    2011-01-01

    The health function on antioxidation and reducing blood lipid of Yizhou Chaenomeles fruit juice was studied.The mice experiments showed serum lipid-lowering and antioxidative action of the Yizhou Chaenomeles fruit juice.Compared with the hyperlipidemic group,Yizhou Chaenomeles fruit juice could significantly lower the cholesterol levels of mice,including the serum TC,TG,LDL-C and arteriosclerosis index(AI).It could also lower the liver weight,TC and TG.Yizhou Chaenomeles fruit juice also improved SOD activity,reduced MDA content in mice and enhanced GSH-Px activity in blood.%利用大白鼠进行动物实验,研究沂州木瓜汁的抗氧化、降血脂保健功能。实验结果表明:沂州木瓜汁能显著降低高血脂大鼠的血清总胆固醇、甘油三脂和低密度脂蛋白水平,减小动脉硬化指标,对大白鼠高脂血症和动脉硬化具有较好的预防作用;沂州木瓜汁还能显著降低高血脂大鼠的肝系数、肝脏总胆固醇和甘油三酯水平,缓解肝脏的脂肪病变;沂州木瓜汁能提高大鼠红细胞内SOD活力、降低红细胞中MDA含量、提高血液中GSH-Px活力,从而清除机体内的超氧阴离子,减少机体受自由基的攻击。

  9. Antihypertensive and lipid lowering treatment in 70–74 year old individuals – predictors for treatment and blood-pressure control: a population based survey. The Hordaland Health Study (HUSK

    Directory of Open Access Journals (Sweden)

    Straand Jørund

    2006-10-01

    Full Text Available Abstract Background In an elderly, community based population we aimed at investigating antihypertensive and lipid lowering medication use in relation to own and familiar cardiovascular morbidity and diabetes mellitus, as well as to lifestyle factors and general health. We also examined levels of blood pressure in untreated and treated residents, to investigate factors correlating with blood pressure control. Methods A health survey carried out in 1997-9 in the county of Hordaland, Norway included a self-administered questionnaire mailed to 4 338 persons born in 1925-7. Drug use the day prior to filling in the questionnaire was reported. A health check-up was carried out, where their systolic and diastolic blood pressure (SBP and DBP, body mass index (BMI, and serum-cholesterol level were recorded. Results One third of respondents used one or more antihypertensive drugs, while 13% of men and women were treated with a statin. Diabetes mellitus, own or relatives'cardiovascular disease, having quit smoking, physical inactivity, and overweight correlated with antihypertensive treatment. Mean blood pressure was lower in respondents not on treatment. Among those on treatment, 38% of men and 29% of women had reached a target BP-level of lower than 140/90 mm Hg. Own cardiovascular disease and a low BMI correlated with good BP-control. Conclusion One third of 70–74 year old individuals living in the community used one or more antihypertensive drugs. Only around one third of those treated had reached a target BP-level of less than 140/90 mm Hg. Own cardiovascular disease and a low BMI correlated with good BP-control.

  10. Diagnostic value of blood lipids testing in patients with high-normal and subclinical levels of TSH in prevention and treatment of dislipoproteinemia

    Directory of Open Access Journals (Sweden)

    O D Rymar

    2010-12-01

    Full Text Available The purpose of the study is to evaluate character of lipid profile changes in Novosibirsk populational sample of men and women (45–69 years with subclinical and high-normal TSH levels. Current study was performed within the HAPIEE project “Determinants of cardiovascular diseases in Eastern Europe” of the Welcome Trust fond. Populational subsample of 280 subjects (125 men (44.6% and 155 women (55.4% was analyzed. Received data showed that high-normal TSH levels within 1.71–4.05 mIU/l have been associated with higher levels of triglycerides, total cholesterol and LDL cholesterol, compared with low-normal TSH levels 0.17–0.47 mIU/l (р<0.05. Women with subclinical hypothyroidism had significantly higher average levels of total cholesterol and LDL cholesterol compared with women without thyroid disorders.

  11. Effects of aerobic interval training versus continuous moderate exercise programme on aerobic and anaerobic capacity, somatic features and blood lipid profile in collegate females

    Directory of Open Access Journals (Sweden)

    Krzystof Mazurek

    2014-11-01

    Full Text Available introduction. Regular physical activity has many positive health benefits, including reducing the risk of cardiovascular diseases, metabolic diseases and some cancers, as well as improving the quality of life. objectives. The aim of the study was to examine the effects of 8-week aerobic interval cycle exercise training (AIT compared to continuous cycle exercises of moderate intensity (CME on the aerobic and anaerobic capacity, somatic features and lipid profile. material and methods. The research was conducted in 88 volunteers aged 19.5±0.6 years, who were randomized to three groups of organized physical activity (OPA, who exercised 3 times per week in 47 min sessions: (I AIT (n=24 comprising 2 series of 6x10 s sprinting with maximal pedalling cadence and active rest pedalling with intensity 65%–75% HRmax, (II CME (n=22 corresponding to 65%-75% HRmax, (III regular collegiate physical education classes of programmed exercises (CON; n=42. Before and after OPA anthropometrics, aero- and anaerobic capacity and lipid profile indices were measured. results. In AIT, a significantly greater decrease of waist circumference and WHR was noted when compared to CON, and a significantly greater reduction of sum of skinfolds than in CON and CME. Improvement in relative and absolute VO2max (L/min and ml/kg/min was significantly higher in AIT than CON. Work output and peak power output in the anaerobic test improved significantly in AIT, CME and CON, but independently of training type. OPA was effective only in reducing triglyceride concentrations in CME and CON groups, without interaction effects in relation to training type. conclusion. It was found that 8 weeks of OPA was beneficial in improving somatic and aerobic capacity indices, but AIT resulted in the greatest improvement in somatic indices (waist circumference, WHR, sum of skinfolds and in VO[sub]2[/sub]max, compared to CME and CON programmes.

  12. Radioprotective effect of Haberlea rhodopensis (Friv.) leaf extract on γ-radiation-induced DNA damage, lipid peroxidation and antioxidant levels in rabbit blood

    International Nuclear Information System (INIS)

    Different concentrations of H. rhodopensis total extract (HRE; 0.03, 0.06 and 0.12 g/kg body weight) were injected im, into rabbits 2 h before collecting the blood samples. The whole blood samples were exposed in vitro to 2.0 Gy 60Co γ-radiation. The radiation-induced changes were estimated by using the chromosome aberration test (CA) and cytokinesis blocked micronucleus assay (CBMN) in peripheral lymphocytes, and by determining the malondialdehyde levels (MDA) in blood plasma and the superoxide dismutase (SOD) and catalase (CAT) activity in erythrocytes. Radiation significantly increased the chromosome aberration and micronuclei frequencies as well as MDA levels and decreased the antioxidant enzyme activity. On the other hand, the HRE pretreatment significantly decreased the CA, MN frequencies and MDA levels and increased the SOD and CAT activity in a concentration dependent manner. The most effective was the highest concentration of HRE (0.12 g/kg body weight). The results suggest that HRE as a natural product with an antioxidant capacity could play a modulatory role against the cellular damage induced by γ-irradiation. The possible mechanism involved in the radioprotective potential of HRE is discussed. (author)

  13. EFSA NDA Panel (EFSA Panel on Dietetic Products, Nutrition and Allergies), 2014. Scientific Opinion on the substantiation of a health claim related to barley soup “Orzotto” and protection of blood lipids from oxidative damage pursuant to Article 13(5) of Regulation (EC) No 1924/2006

    DEFF Research Database (Denmark)

    Tetens, Inge

    coloured” spinach, “tri-coloured” Swiss chard, onions, red and yellow celery and parsley, is sufficiently characterised. The claimed effect, protection of blood lipids from oxidative damage, may be a beneficial physiological effect. No human intervention studies from which conclusions could be drawn for...

  14. Alterations in the blood glucose, serum lipids and renal oxidative stress in diabetic rats by supplementation of onion (Allium cepa. Linn)

    OpenAIRE

    Bang, Mi-Ae; Kim, Hyeon-A; Cho, Young-Ja

    2009-01-01

    This study examined the anti-diabetic effect of onion (Allium cepa. Linn) in the streptozotocin (STZ)-induced diabetic rats. Male Sprague-Dawley rats were divided into normal rats fed control diet or supplemented with onion powder (7% w/w) and diabetic rats fed control diet or supplemented with onion powder. Diabetes was induced by a single injection of STZ (60 mg/kg, ip) in citrate buffer. The animals were fed each of the experimental diet for 5 weeks. Blood glucose levels of rats supplement...

  15. Prenatal, but not early postnatal, exposure to a Western diet improves spatial memory of pigs later in life and is paired with changes in maternal prepartum blood lipid levels.

    Science.gov (United States)

    Clouard, Caroline; Kemp, Bas; Val-Laillet, David; Gerrits, Walter J J; Bartels, Andrea C; Bolhuis, J Elizabeth

    2016-07-01

    Maternal obesity and perinatal high-fat diets are known to affect cognitive development. We examined the effects of late prenatal and/or early postnatal exposure to a Western-type diet, high in both fat and refined sugar, on the cognition of pigs (Sus scrofa) in the absence of obesity. Thirty-six sows and their offspring were assigned to 1 of 4 treatments in a 2 × 2 factorial arrangement, with 8 wk prenatal and 8 wk postnatal exposure to a Western diet (enriched in fat, sucrose, and cholesterol) or control diets as factors. Compared to controls, piglets exposed to the prenatal Western diet showed enhanced working and reference memory during the acquisition and reversal phases of a spatial hole-board task. Mothers fed the prenatal Western diet had higher prepartum blood cholesterol and free fatty acid levels. Postnatal exposure to the Western diet did not affect piglet cognitive performance, but it did increase postpartum maternal and postweaning piglet cholesterol levels. The Western diet had no effect on maternal or offspring insulin sensitivity or leptin levels. In conclusion, a prenatal Western diet improved memory function in pigs, which was paired with changes in prepartum maternal blood cholesterol levels. These findings highlight the key role of late fetal nutrition for long-term programming of cognition.-Clouard, C., Kemp, B., Val-Laillet, D., Gerrits, W. J. J., Bartels, A. C., Bolhuis, J. E. Prenatal, but not early postnatal, exposure to a Western diet improves spatial memory of pigs later in life and is paired with changes in maternal prepartum blood lipid levels. PMID:26985006

  16. Effects of taurine intake on serum lipids in young women

    Directory of Open Access Journals (Sweden)

    Sadako Matsui

    2015-04-01

    Full Text Available Background: Taurine is an abundant amino acid in human cells, promoting ocular and biliary health, which is also used to treat congestive heart failure, hypertension, and hepatitis. Recently, taurine-enriched energy drinks have become popular with young adults, but the effects of taurine on serum lipids in young adults are unknown. Objective: We studied the influence of oral administration of taurine on serum lipid levels in healthy young women. Methods: Ten healthy young women with a mean body mass index of 20.0kg/m2, apolipoprotein E (apoE phenotype 3/3 and normal menstrual cycles participated. Each subject was instructed to orally ingest 1g of taurine powder after each meal (3g/day in addition to their usual diets during one menstrual cycle. Before and at the end of taurine intake, physical measurements and blood collection were performed in the morning after a 12-h fast, and 3-day weighted dietary records were obtained. Concentrations of serum lipids, apolipoproteins, and fatty acids in the serum phospholipid fraction were measured. Results: The subjects showed good compliance with taurine intake and none reported adverse effects during the experimental period. After taurine intake, concentrations of total cholesterol, low density lipoprotein cholesterol (LDL-C, free cholesterol, and apolipoprotein B (apoB increased (p<0.05, while phospholipids tended to increase (p=0.051. Fatty acids in the serum phospholipid fraction also significantly increased (p<0.05. However, triglyceride, remnant-like particle cholesterol, remnant-like particle triglyceride, apoE, the apolipoprotein A-1 (apoA- 1/apoB ratio and the LDL-C/apoB ratio were unchanged. Furthermore, body weight was significantly increased (p<0.01, but did not correlate with changes either in serum lipids or nutrient intakes. Conclusion: These results suggest that high taurine intake affects lipoprotein metabolism and increases serum lipids in slightly lean young women.

  17. 成人身体成分与血脂、血糖关系的探讨%Analysis of the relationship between body composition and fasting serum lipids, fasting blood glucose in adult

    Institute of Scientific and Technical Information of China (English)

    李秋桂; 姬芳勤

    2014-01-01

    目的:分析成人身体成分与血脂、血糖指标之间的关系方法选择成年体检者490名,排除严重心脑肝肾疾病,肿瘤患者,急性炎症患者及长期服药者等。通过Biospace InBody 220人体成分分析仪对身体水分、蛋白质、无机盐、体脂肪、体重、骨骼肌肉量、体质指数、体脂百分比、腰臀脂肪比等指标进行测定。同时测定空腹血糖、血脂指标。所有指标的测定均由我院医护人员经过统一培训后进行操作。结果男女性别年龄无差异(p>0.05)。除体脂肪外,男女性别间身体水分、蛋白质、无机盐、体重、骨骼肌肉量、体质指数、体脂百分比、腰臀脂肪比均有显著差异(均p<0.001)。蛋白质、体脂肪、体重、骨骼肌肉量、体质指数、体脂百分比、腰臀脂肪比与空腹血糖呈正相关(均p<0.05)。身体水分、蛋白质、无机盐、体脂肪、体重、骨骼肌肉量、体质指数、体脂百分比、腰臀脂肪比均可对血脂(总胆固醇、甘油三酯)产生影响(均p<0.05)。剔除性别因素后,对身体成分与血糖、血脂各指标进行偏相关分析,体脂肪、体重、体质指数、体脂百分比、腰臀脂肪比仍然与血糖、总胆固醇、甘油三酯呈正相关(均p<0.05)。结论成人身体成分与血糖、血脂指标密切相关。通过身体成分的分析,可尽早预防代谢相关性疾病。%Objective To analyze the relationship between body composition and fasting serum lipids, fasting blood glucose in adult. Methods Select 490 adult, eliminate the subjects who has the serious cardiovascular diseases, liver diseases , kidney diseases, cancer and the subjects with acute inflammation and long term medication etc. Body moisture, protein, inorganic salt, fat, skeletal muscle weight, body mass index, percentage of body fat, waist and hip fat ratio was determined by Biospace InBody 220. Measure the

  18. Efeitos da farinha de folhas de mandioca sobre a peroxidação lipídica, o perfil lipídico sangüíneo e o peso do fígado de ratos Effects of cassava leaf flour on lipidic peroxidation, blood lipidic profile and liver weight of rats

    Directory of Open Access Journals (Sweden)

    Daniela Séfora de Melo

    2007-04-01

    Full Text Available Sabe-se que polifenóis e saponinas apresentam efeitos antioxidante e hipolipidêmico, respectivamente. Como folhas de mandioca contêm estas substâncias, foi investigado o efeito de dietas contendo a farinha de folhas de mandioca (FFM sobre a peroxidação lipídica, o perfil lipídico sangüíneo e o peso do fígado de ratos. Para isto, folhas maduras de mandioca (Manihot esculenta Crantz cv. Cacao foram secas em estufa ventilada a 30-35 ºC e trituradas sem os pecíolos. Um ensaio biológico, com 32 ratos machos Wistar, foi conduzido por um período de 7 semanas com quatro tratamentos: dieta controle e dietas contendo 5%, 10% e 15% de FFM, sendo todas suplementadas com 1% de colesterol. As dietas se apresentaram isoenergéticas e contendo os mesmos níveis de proteína digestível e fibras. Foi observado que dietas contendo FFM não apresentaram efeitos significativos sobre o perfil lipídico sangüíneo e sobre as variáveis de desempenho, mas contribuiram para o aumento no peso do fígado. Já as dietas contendo 10% e 15% de FFM foram associadas à redução nos teores plasmáticos de substâncias reativas ao ácido tiobarbitúrico.It is well known that polyphenols and saponins present antioxidant and hypolipidemic effects, respectively. As cassava leaves contains these substances, the effects of diets supplemented with cassava leaf flour (CLF on lipidic peroxidation, blood lipid profile and liver weight of rats were investigated. Therefore, ripe cassava leaves (Manihot esculenta Crantz cv. Cacao were dried in a ventilated oven at 30-35 ºC and ground without the petioles. A biological assay was conducted during 7 weeks with 32 Wistar male rats submitted to four treatments: control diet and diets containing 5%, 10% and 15% CLF, all supplemented with 1% cholesterol. Diets were isoenergetic and contained the same levels of digestible protein, and fibers. It was observed that diets containing CLF presented no significant effect on blood

  19. The Effects of Probiotic Soymilk Fortified with Omega-3 on Blood Glucose, Lipid Profile, Haematological and Oxidative Stress, and Inflammatory Parameters in Streptozotocin Nicotinamide-Induced Diabetic Rats

    Directory of Open Access Journals (Sweden)

    Mohsen Mohammadi Sartang

    2015-01-01

    Full Text Available Objective. The aim of the present study was to evaluate the effects of probiotic soymilk fortified with omega-3 in diabetic rats. Methods. Soymilk (SM, fermented soymilk (FSM, and fermented soymilk fortified with omega-3 (FSM + omega-3 were prepared. Rats were randomly assigned to five groups of 13 animals per group. Diabetes was induced by a single injection of streptozotocin (STZ 15 min after the intraperitoneal administration of nicotinamide (NA. Normal control (NC and diabetic control (DC rats received 1 mL/day of distilled water and three groups of diabetic rats were given 1 mL/day of SM, FSM, and FSM + omega-3 products by oral gavage for 28 days. Results. Three products significantly (P<0.05 reduced blood glucose, total cholesterol (TC, triglyceride (TG, and malondialdehyde (MDA concentrations compared to the DC group, with the maximum reduction seen in the FSM + omega-3 group. Body weight, red blood cells (RBC, haemoglobin (Hb, haematocrit, and superoxide dismutase (SOD also significantly increased in the FSM + omega-3 group. In the FSM + omega-3 group, MDA level compared with the SM and FSM groups and high sensitivity C-reactive protein (hs-CRP concentrations compared with the DC and FSM groups were significantly lower (P<0.05. Conclusion. Fermented soymilk fortified with omega-3 may be beneficial in diabetes.

  20. Improvement of Blood Pressure, Glucose Metabolism, and Lipid Profile by the Intake of Powdered Asparagus ( Lú Sŭn) Bottom-stems and Cladophylls.

    Science.gov (United States)

    Nishimura, Mie; Ohkawara, Tatsuya; Kagami-Katsuyama, Hiroyo; Sato, Hiroji; Nishihira, Jun

    2013-10-01

    Asparagus ( Lú Sǔn; Asparagus officinalis L.) is a common vegetable, long used as an herbal medicine. The cladophylls and bottom-stems of asparagus have various pharmacological effects, but they are generally discarded at harvesting. The present open clinical trial was performed to examine the effects of the intake of cladophylls and bottom-stems on the improvement of metabolic syndrome characterized by hypertension, hyperglycemia, and dyslipidemia. Twenty-eight healthy volunteers ingested either cladophyll or bottom-stem powder (6 g/day) daily for 10 weeks. The cladophyll intake resulted in significant reduction in the subjects' diastolic blood pressure and fasting plasma glucose (FPG), and decreased both the left cardio-ankle vascular index score and the total cholesterol level (T-CHO). The bottom-stem intake significantly reduced the subjects' systolic and diastolic blood pressure and FPG as well as T-CHO. These results suggest the possibility that asparagus cladophylls and bottom-stems differentially improve hypertension, hyperglycemia, and dyslipidemia. PMID:24716185

  1. Fluorescence lifetime imaging of lipids during 3T3-L1 cell differentiation

    Science.gov (United States)

    Song, Young Sik; Won, Young Jae; Lee, Sang-Hak; Kim, Dug Young

    2014-03-01

    Obesity is becoming a big health problem in these days. Since increased body weight is due to increased number and size of the triglyceride-storing adipocytes, many researchers are working on differentiation conditions and processes of adipocytes. Adipocytes also work as regulators of whole-body energy homeostasis by secreting several proteins that regulate processes as diverse as haemostasis, blood pressure, immune function, angiogenesis and energy balance. 3T3-L1 cells are widely used cell line for studying adipogenesis because it can differentiate into an adipocyte-like phenotype under appropriate conditions. In this paper, we propose an effective fluorescence lifetime imaging technique which can easily distinguish lipids in membrane and those in lipid droplets. Nile red dyes are attached to lipids in 3T3-L1 cells. Fluorescence lifetime images were taken for 2 week during differentiation procedure of 3T3-L1 cells into adipocytes. We used 488 nm pulsed laser with 5MHz repetition rate and emission wavelength is 520 nm of Nile Red fluorescent dye. Results clearly show that the lifetime of Nile red in lipid droplets are smaller than those in cell membrane. Our results suggest that fluorescence lifetime imaging can be a very powerful tool to monitor lipid droplet formation in adipocytes from 3T3-L1 cells.

  2. Diet Quality and Change in Blood Lipids during 16 Years of Follow-up and Their Interaction with Genetic Risk for Dyslipidemia

    Directory of Open Access Journals (Sweden)

    Emily Sonestedt

    2016-05-01

    Full Text Available A high diet quality according to the Swedish nutrition recommendations is associated with a reduced risk of cardiovascular disease in the population-based Malmö Diet and Cancer cohort. To further clarify this protective association, we examined the association between high diet quality and change in triglycerides, high density lipoprotein-cholesterol (HDL-C, and low density lipoprotein-cholesterol (LDL-C after 16 years of follow-up in 3152 individuals (61% women; 46–68 years at baseline. In addition, we examined if genetic risk scores composed of 80 lipid-associated genetic variants modify these associations. A diet quality index based on intakes of saturated fat, polyunsaturated fat, sucrose, fiber, fruit and vegetables, and fish was constructed. A high diet quality was associated with lower risk of developing high triglycerides (p = 0.02 and high LDL-C (p = 0.03 during follow-up compared with a low diet quality. We found an association between diet quality and long-term change in HDL-C only among those with lower genetic risk for low HDL-C as opposed to those with higher genetic risk (p-interaction = 0.04. Among those with lower genetic risk for low HDL-C, low diet quality was associated with decreased HDL-C during follow-up (p = 0.05. In conclusion, individuals with high adherence to the Swedish nutrition recommendation had lower risk of developing high triglycerides and LDL-C during 16 years of follow-up.

  3. Effects of butter naturally enriched with conjugated linoleic acid and vaccenic acid on blood lipids and LDL particle size in growing pigs

    Directory of Open Access Journals (Sweden)

    Haug Anna

    2008-08-01

    Full Text Available Abstract Background Cow milk is a natural source of the cis 9, trans 11 isomer of conjugated linoleic acid (c9,t11-CLA and trans vaccenic acid (VA. These fatty acids may be considered as functional foods, and the concentration in milk can be increased by e.g. sunflower oil supplementation to the dairy cow feed. The objective of this study was to compare the effects of regular butter with a special butter naturally enriched in c9,t11-CLA and VA on plasma lipids in female growing pigs. The experimental period lasted for three weeks and the two diets provided daily either 5.0 g c9,t11-CLA plus 15.1 g VA or 1.3 g c9,t11-CLA plus 3.6 g VA. Results The serum concentrations of c9,t11-CLA, VA and alpha-linolenic acid were increased and myristic (14:0 and palmitic acid (16:0 were reduced in the pigs fed the CLA+VA-rich butter-diet compared to regular butter, but no differences in plasma concentrations of triacylglycerol, cholesterol, HDL-cholesterol, LDL-cholesterol, LDL particle size distribution or total cholesterol/HDL cholesterol were observed among the two dietary treatment groups. Conclusion Growing pigs fed diets containing butter naturally enriched in about 20 g c9,t11-CLA plus VA daily for three weeks, had increased serum concentrations of alpha-linolenic acid and decreased myristic and palmitic acid compared to pigs fed regular butter, implying a potential benefit of the CLA+VA butter on serum fatty acid composition. Butter enriched in CLA+VA does not appear to have significant effect on the plasma lipoprotein profile in pigs.

  4. A comparative study on genetic and environmental influences on metabolic phenotypes in Eastern (Chinese) and Western (Danish) populations

    DEFF Research Database (Denmark)

    Li, Shuxia

    2015-01-01

    During the last decades metabolic disorders such as high blood pressure, impaired blood glycose, atherosclerotic lipid abnormalities, overweight/obesity and elevated blood pressure are among the leading risks for mortality and morbidity worldwide. Those risks are mostly responsible for raising...... and environmental factors play important role in the variation of metabolic phenotypes and intra-individual change over time. Although both genetic and environmental factors are involved the development of metabolic disorders, the role of environment should be emphasized as the expression or function of gene can...... be regulated to adapt to existing environmental circumstance. In other words, adaptive evolution in populations under distinct environmental and cultural circumstances could have resulted in varying genetic basis of metabolic factors and development of metabolic disorders or diseases. Thus, it can...

  5. 体质指数、血脂水平与前列腺穿刺活检阳性间的关系%Relationship of body mass index and blood lipid level with cancer detection on prostate biopsy

    Institute of Scientific and Technical Information of China (English)

    张浩宇; 吕广霖; 袁和兴; 魏雪栋; 胡林昆; 张学锋; 侯建全

    2015-01-01

    Objective To investigate the relationship between body mass index (BMI), blood lipid level and cancer de⁃tection in prostate biopsy. Methods A total of 214 patients undergoing a prostate biopsy during 2013.2—2014.8 were re⁃viewed retrospectively. They were divided into prostate cancer and non-cancer groups by biopsy results. The differences of age, prostate-specific antigen (PSA) level, prostate volume (PV), blood lipid level and BMI were analyzed between two groups. Risk factors for cancer detection of biopsy were also analyzed. Results Compared with non-cancer patients, pros⁃tate cancer patients were older, had higher level of PSA and BMI, but smaller PV and lower level of HDL-C ( P<0.05). Lo⁃gistic regression analysis showed that older, higher level of PSA and BMI were risk factors for prostate biopsy positive, but larger PV and higher level of HDL-C were protective factors (P<0.05). Conclusion Comprehensive assessment of BMI and blood lipid levels can provide important reference for prostate cancer screening at early time and establishment of pros⁃tate biopsy scheme, which also provide significant evidence for the prevention, diagnosis and treatment of prostate cancer at early stage for high risk population.%目的:探讨体质指数(BMI)、血脂水平与前列腺穿刺活检阳性之间的关系。方法回顾性分析2013年2月—2014年8月间在我院行前列腺穿刺活检患者214例,根据穿刺病理结果分为前列腺癌组和非癌组,比较2组间年龄、前列腺特异性抗原(PSA)、前列腺体积(PV)、血脂水平、体质指数(BMI)等指标,并分析前列腺穿刺阳性的危险因素。结果前列腺癌组年龄、PSA、BMI高于非癌组,而PV、高密度脂蛋白胆固醇(HDL-C)则低于非癌组(均P<0.05)。Logistic回归分析提示年龄大,PSA、BMI升高是前列腺穿刺活检阳性的危险因素,而PV、HDL-C升高则为保护因素(均P<0.05)。结论综合评估患者BMI

  6. CS/GA鱼油微胶囊辅助降血脂功能研究%Effect of CS/GA fish oil microcapsule on reducing blood lipid of rat

    Institute of Scientific and Technical Information of China (English)

    石龙贵; 齐玉刚; 刘彦平; 滕安国; 刘安军; 张强

    2013-01-01

    Gum acacia(GA) and chitosan (CS) used as wall materials and fish oil used as core material,CS/GA fish oil microcapsule was prepared by complex coacervation.Using Kunming rats as the test animals and feeding them with high-fat diet,hyperlipidemia rats model was established.Hyperlipidemia model group,normal control group,CS/GA wall material group,fish oil microcapsule group and fish oil group were set.Then the concentrations of serum triglyceride (TG),total cholesterol (TC),high-density lipoprotein cholesterol (HDL-C),low-density lipoprotein cholesterol (LDL-C) and the changes of rats' weights were determined after feeding for 28 days to investigate the effects of fish oil microcapsule and fish oil on blood lipid of rat in the process of forming hyperlipidemia.The results showed that CS/GA complex condensate wall material had no blood lipid regulating function,while the concentrations of TC and LDL-C of rats in fish oil microcapsule group and fish oil group were significantly lower than hyperlipidemia model group(P < 0.01) and HDL-C concentrations were remarkably higher than hyperlipidemia model group(P <0.01),which indicated that fish oil microcapsule and fish oil had the same function of reducing blood lipid cholesterol level.%以鱼油为芯材,以阿拉伯胶(GA)和壳聚糖(CS)为壁材,采用复凝聚法制备鱼油微胶囊.以昆明小鼠为供试动物,喂食高脂饲料建立高血脂症模型,设置高脂模型组、正常对照组、CS/GA壁材组、鱼油组和鱼油微胶囊组.喂养28 d后测定小鼠血清中甘油三酯(TG)、总胆固醇(TC)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)以及小鼠体重的变化,以考察鱼油微胶囊与液态鱼油在小鼠形成高血脂症过程中对血脂的影响.结果表明:单纯的CS/GA壁材复凝聚物对血脂调节没有效果,喂食鱼油微胶囊和鱼油组小鼠TC、LDL-C均极显著低于高脂模型组(P<0.01),且HDL-C均极显著高于高脂模型组(P<0

  7. Blood pressure and fasting lipid changes after 24 weeks’ treatment with vildagliptin: a pooled analysis in >2,000 previously drug-naïve patients with type 2 diabetes mellitus

    Directory of Open Access Journals (Sweden)

    Evans M

    2016-08-01

    Full Text Available Marc Evans,1 Anja Schweizer,2 James E Foley3 1Diabetes Resource Centre, Llandough Hospital, Cardiff, UK; 2Medical Affairs Cardio Metabolic, Novartis Pharma AG, Basel, Switzerland; 3Medical Affairs Cardio-Metabolic, Novartis Pharmaceuticals Corporation, East Hanover, NJ, USA Introduction: We have previously shown modest weight loss with vildagliptin treatment. Since body weight balance is associated with changes in blood pressure (BP and fasting lipids, we have assessed these parameters following vildagliptin treatment. Methods: Data were pooled from all double-blind, randomized, controlled, vildagliptin monotherapy trials on previously drug-naïve patients with type 2 diabetes mellitus who received vildagliptin 50 mg once daily (qd or twice daily (bid; n=2,108 and wherein BP and fasting lipid data were obtained. Results: Data from patients receiving vildagliptin 50 mg qd or bid showed reductions from baseline to week 24 in systolic BP (from 132.5±0.32 to 129.8±0.34 mmHg; P<0.0001, diastolic BP (from 81.2±0.18 to 79.6±0.19 mmHg; P<0.0001, fasting triglycerides (from 2.00±0.02 to 1.80±0.02 mmol/L; P<0.0001, very low density lipoprotein cholesterol (from 0.90±0.01 to 0.83±0.01 mmol/L; P<0.0001, and low density lipoprotein cholesterol (from 3.17±0.02 to 3.04±0.02 mmol/L; P<0.0001, whereas high density lipoprotein cholesterol increased (from 1.19±0.01 to 1.22±0.01 mmol/L; P<0.001. Weight decreased by 0.48±0.08 kg (P<0.001. Conclusion: This large pooled analysis demonstrated that vildagliptin shows a significant reduction in BP and a favorable fasting lipid profile that are associated with modest weight loss. Keywords: TG, HDL, LDL, body weight DPP-4 inhibitor, GLP-1 

  8. Defective lipid metabolism in sickle cell anaemia subjects in vaso-occlusive crisis

    Directory of Open Access Journals (Sweden)

    Kehinde Sola Akinlade

    2014-01-01

    Full Text Available Background: Abnormal lipid homeostasis has been reported in sickle cell anaemia (SCA as well as in other haematological disorders. However, there is little information on the lipid profile of SCA subjects in vaso-occlusive crisis (VOC. This study determined the lipid profile of adult SCA subjects in VOC and in steady state (SSCA. Materials and Methods: Fifty-eight (58 adults with HbSS (30 in steady state and 28 in vaso-occlusive crisis and 24 age-matched healthy individuals with HbAA genotype were recruited into this study. Standard methods were used for the determination of blood pressure (BP, packed cell volume (PCV, total white blood cell count (WBC and haemoglobin phenotype. After an overnight fast, 5 ml of venous blood was obtained from each SSCA and the controls while samples were collected upon admission in the VOC group. Plasma lipid profile was determined using enzymatic method. Differences between two groups were determined using independent Student′s t-test or Man-Whitney U as appropriate. P-values less than 0.05 were considered significant. Results: Plasma total cholesterol (TC and high density lipoprotein (HDL were significantly lower while the ratio of triglyceride (TG to HDL (TG/HDL was significantly higher in SSCA compared with the controls. Low density lipoprotein (LDL and TC were significantly lower in SCA subjects in VOC compared with controls. However, TC, TG, LDL and TG/HDL were significantly lower while HDL was significantly higher in VOC compared with SSCA. Conclusion: Sickle cell anaemia subjects have defective fasting lipid metabolism which becomes pronounced with VOC.

  9. 泰山沙棘果提取物调血脂作用研究%Study on Regulating Blood Lipid Effect of the Extracts of Taishan Hippophae rhamnoides Fruits

    Institute of Scientific and Technical Information of China (English)

    张颖; 张立木; 秦树存; 李同德; 彭吉星

    2011-01-01

    目的:研究沙棘果醇提物和水提物对高脂血症模型小鼠的调血脂作用.方法:以喂饲普通饲料作为正常对照组,以高脂血症小鼠作为模型组,以1000、500和250 mg·kg-1ig剂量的沙棘果醇提物和水提物为实验组,测定血清中的总胆固醇(TC)、甘油三酯(TG)、高密度脂蛋白(HDL-C)含量.结果:模型组TC、TG水平显著高于正常对照组(P<0.01);与模型组比较,不同剂量沙棘果醇提物可显著降低TC水平(P<0.01);不同剂量沙棘果醇提物与水提物可显著降低TG水平(P<0.05或P<0.01),对高脂血症模型小鼠血清中HDL-C无升高作用.结论:沙棘果提取物具有明显的调血脂作用,可用于高脂血症的预防和治疗.%OBJECTIVE:To explore the effect of the ethanol extract and water extract of Hippophae rhamnoides fruits on regulating blood lipid in hyperlipemia mice. METHODS: Normal control group was treated with common diet, experimental hyperlipemia mice were model group. The hyperlipemia mice were raised with ethanol extract and water extract of H. rhamnoides fruits at the dose of 1 000, 500 and 250 mg·kg-1 as experiment group. The levels of total chholesterin(TC),triglyeride (TG),high lipoprotein cholesterol (HDL-C) in serum were all tested. RESULTS: The level of TC and TG in model group were higher than in normal control group(P<0.01).Compared with model group, the level of the TC decreased significantly in the different dose ethanol extract of H. rhamnoides fruits group (P<0.01). The level of TG decreased significantly in different dose of ethanol extract and water extract of H. rhamnoides fruits (P<0.05 or P<0.01), and it was not obvious effect on the level of HDL-C in serum. CONCLUSION: The extracts of H. rhamnoides fruits have significantly effects on regulating blood lipid. It can prevent and cure the hyperlipemia.

  10. Lipid Peroxidation and Antioxidant Status in Preeclampsia

    OpenAIRE

    S.V. Kashinakunti; Sunitha H; K. Gurupadappa; D.S. Shankarprasad; G.Suryaprakash; J.B Ingin

    2010-01-01

    The current theory suggests that in preeclampsia there is an increase in the lipid peroxidation products and leads to decrease in the serum antioxidants except uric acid, contributing to the pathogenesis of preeclampsia. In this context, this study was undertaken to determine the changes in blood levels of lipid peroxide and antioxidant levels in women with preeclampsia.Method: Case control study consisting of 30 preeclamptic and 30 healthy pregnant women. Fasting venous blood samples wer...

  11. Effects of different fibre sources and fat addition on cholesterol and cholesterol-related lipids in blood serum, bile and body tissues of growing pigs.

    Science.gov (United States)

    Kreuzer, M; Hanneken, H; Wittmann, M; Gerdemann, M M; Machmuller, A

    2002-04-01

    Knowledge is limited on the efficacy of hindgut-fermentable dietary fibre to reduce blood, bile and body tissue cholesterol levels. In three experiments with growing pigs the effects of different kinds and levels of bacterially fermentable fibre (BFS) on cholesterol metabolism were examined. Various diets calculated to have similar contents of metabolizable energy were supplied for complete fattening periods. In the first experiment, a stepwise increase from 12 to 20% BFS was performed by supplementing diets with fermentable fibre from sugar beet pulp (modelling hemicelluloses and pectin). Beet pulp, rye bran (modelling cellulose) and citrus pulp (pectin) were offered either independently or in a mixture in the second experiment. These diets were opposed to rations characterized in carbohydrate type by starch either mostly non-resistant (cassava) or partly resistant (maize) to small intestinal digestion. The third experiment was planned to explore the interactions of BFS from citrus pulp with fat either through additional coconut oil/palm kernel oil blend or full-fat soybeans. In all experiments the increase of the BFS content was associated with a constant (cellulose) or decreasing (hemicelluloses, pectin) dietary proportion of non-digestible fibre. In experiment 1 an inverse dose-response relationship between BFS content and cholesterol in blood serum and adipose tissue as well as bile acid concentration in bile was noted while muscle cholesterol did not respond. In experiment 2 the ingredients characterized by cellulose and hemicelluloses/pectin reduced cholesterol-related traits relative to the low-BFS-high-starch controls whereas, except in adipose tissue cholesterol content, the pectinous ingredient had the opposite effect. However, the changes in serum cholesterol mainly affected HDL and not LDL cholesterol. Adipose tissue cholesterol also was slightly lower with partly resistant starch compared to non-resistant starch in the diet. Experiment 3 showed that

  12. 水母雪莲细胞培养物调血脂作用的研究%Effects of cell cultures of saussurea medusa in regulating blood lipid of hyperlipidemic rats

    Institute of Scientific and Technical Information of China (English)

    杨波; 李天德; 胡显文; 王劲松; 高丽华; 陈惠鹏

    2004-01-01

    背景:鉴于野生水母雪莲濒临灭绝,军事医学科学院建立了一种人工培养水母雪莲的方法.目的:探讨水母雪莲细胞培养物对高脂大鼠的调血脂作用.设计:完全随机设计,对照实验研究.单位:由解放军总医院心内科和军事医学科学院生物工程研究所合作.材料:实验于2003-03/06在解放军总医院医学实验动物中心完成.实验动物由解放军总医院实验动物中心提供.雄性SD大鼠64只,体质量200~220 g,随机分为4组,每组16只:正常对照组、高脂模型组、高剂量组和低剂量组.干预:正常对照组仅喂基础饲料,高脂模型组喂高脂饲料,高剂量组喂高脂饲料的同时饲以水母雪莲细胞培养物500 mg/Kg,低剂量组喂高脂饲料的同时饲以水母雪莲细胞培养物50 mg/Kg.给药1次/d,3周后采血,测定血脂水平及肝肾功能.主要观察指标:各组间血脂水平差异及肝肾功能变化.结果:高剂量组胆固醇、三酰甘油、低密度脂蛋白水平低于高脂模型组[(2.66±0.51)mmol/L,(3.23±0.57)mmol/L,P=0.030;(1.59±0.77)mmol/L,(2.48±0.83)mmol/L,P=0.023;(1.68±0.31)mmol/L,(2.07±0.43)mmol/L,P=0.032].高剂量组高密度脂蛋白水平高于高脂模型组[(0.98±0.29)mmol/L,(0.66±0.37)mmol/L,P=0.045].各组间肝肾功能差异无显著性意义(P>0.05).结论:水母雪莲细胞培养物对高脂大鼠具有调血脂的作用.%BACKGROUND: Wild saussurea medusa is at the edge of extinction. For this reason, the Academy of Military Medical Sciences has established a method to culture high-yield S. medusa cell lines.OBJECTIVE: To investigate the effects of the cell lines of saussurea medusa(CLSM) on regulating blood lipid in rats with hyperlipidemia.DESIGN: A completely randomized controlled experimental study.SETTING: This study was done by the Department of Cardiology in the General Hospital of Chinese PLA and the Institute of Biotechnology in Academy of Military Medical Sciences.PARTICIPANTS: The

  13. Effects of dietary n-3 fatty acids and vitamin C on semen characteristics, lipid composition of sperm and blood metabolites in fat-tailed Moghani rams.

    Science.gov (United States)

    Jafaroghli, M; Abdi-Benemar, H; Zamiri, M J; Khalili, B; Farshad, A; Shadparvar, A A

    2014-06-10

    Sixteen fertile rams were randomly allotted to four groups and fed either of the four diets for 14 weeks: (1) control diet (COD) without fish oil (FO) and vitamin C (VC), (2) diet containing 2.5% FO (FOD), (3) diet containing 300 mg/kg DM VC (VCD), and (4) diet containing 2.5% FO and 300 mg/kg DM VC (FCD). Semen was collected at 14-d intervals from 1 April to 10 July (out of the physiologic breeding season in Iran). Semen volume and percentages of motile and progressively motile sperm were increased by FO and VC feeding. A significant interaction was also found between FOD and VCD on motility and progressive motility percentage (P<0.05). HOS-test and percentage of sperm with normal acrosome improved significantly by FO and VC. Rams fed FCD had better HOS-test and higher proportion of sperm with normal acrosome than rams in other groups (82.4 and 93.6%, respectively). Diets containing FO and FO and VC increased the proportion of docosahexaenoic acid in sperm (P<0.05). The activity of lactate dehydrogenase in the seminal fluid was significantly affected by VC and the interaction between FO and VC (P<0.05). Blood metabolites, except glucose, were affected positively by FO. The results showed that dietary supplementation with FO and VC improved seminal quality and may have beneficial effects on fertility in Moghani rams. PMID:24745668

  14. The clinical study of the relationship between obesity and blood glucose,blood lipids,sleep apnea syndrome%肥胖与血糖、血脂、睡眠呼吸暂停综合征关系的临床研究

    Institute of Scientific and Technical Information of China (English)

    陆伟荣; 张玉荣; 王晓薇

    2011-01-01

    目的 了解肥胖程度与血糖、血脂、睡眠呼吸暂停综合征(SAS)的关系.方法 选取2009年1月~2010年2月期间本院收治的181例SAS患者接受睡眠图测评,根据体质量指数(BMI),患者被划为正常体质量、超重、肥胖组,记录SAS相关指标睡眠呼吸事件指数(AHI),平均呼吸暂停时间,并测定研究对象的BMI、空腹血糖(FBG)、甘油三脂(TC)、总胆固醇(TG)、低密度脂蛋白(LDL-C)、高密度脂蛋白(HDL-C).结果 随着BMI指数的递增,FBG逐渐升高,TC、TG、LDL-C逐步增加,而HDL-C逐步减低,SAS严重程度也逐渐加重,呼吸暂停时间延长.结论 控制体质量是治疗SAS的基本措施,同时对血糖、血脂的代谢也起至关重要的作用.%Objective To study the relationship between obesity and blood glucose, blood lipids, sleep apnea syndrome (SAS). Methods SAS Sleep map evaluation of 181 patients who have been received in our hospital from Jan. 2009 to Feb. 2010 was collected. According to body mass index (BMI), patients were divided into three groups: normal weight group, overweight group and obesity group. Indicators of SAS- related sleep apnea event index (AHI), and mean apnea time were recorded. BMI, fasting blood glucose (FBG), triglycerides (TC), total cholesterol (TG),low density lipoprotein (LDL - C), high density lipoprotein ( HDL - C) were also measured. Resuits As BMI raising, FBG, TC, TG and LDL- C levels significantly increased, while HDL- C decreased. SAS trends to be more severer, and the duration of apnea was prolonged. Conclusion The basic treatment of SAS was to control weight, which also plays a vital role in regulating blood glucose and lipid metabolism.

  15. 亚麻木酚素对去卵巢大鼠血脂水平的影响%EFFECTS OF SECOISOLARICIRESINOL DIGLUCOSIDE ON BLOOD LIPIDS IN OVARIECTOMIZED RATS

    Institute of Scientific and Technical Information of China (English)

    那晓琳; 赵新宇; 李丽娜; 张云波; 陈文华; 管春梅

    2012-01-01

    Objective To observe the influence of secoisolariciresinol diglucoside (SDG) on serum lipid levels in ovariectomized rats. Methods Eight-week female SD rats were randomly divided into 4 groups according to their weight. After removing the ovaries (OVX) or performing sham operation, all rats were feed high-fat-diet for 1w and then the experimental groups were gived different concentrations of SDG for 8 w. Every 2 w, the blood was taken for measuring blood lipids. At the end of the experiment, all rats were sacrificed and the important organs were taken and weighed. The uterine samples were taken for pathology analysis. Results Compared with OVX control group, high-dose of SDG ( 75mg/kg bw d) could inhibit the uterine atrophy in a certain extent. There were no effects of SDG on liver, spleen and kidney index, but thymus-imdex was increased significantly (P0.05). High-dose of SDG reduced the serum total cholesterol significantly (P<0.05). Conclusions SDG can inhibit uterus atrophy, increase thymus index and regulate rerum lipids in ovariectomized rats.%目的 研究亚麻木酚素(secoisolariciresinol diglucoside,SDG)对去卵巢大鼠血脂水平的影响.方法 以8w龄雌性SD大鼠为研究对象,根据体重随机分为4组,分别为假手术组(SHAM)、去卵巢对照组(oVc)、低剂量和高剂量亚麻木酚素组(L-SDG、H-SDG).手术后高脂饲料喂养1w,然后每日灌胃给予不同浓度的SDG.每2w取动物尾血分离血清测定血脂含量,喂养8w后处死大鼠,采集重要脏器称重并对子宫样本进行病理分析.结果 与去卵巢对照组相比,高剂量的SDG(75mg/kg bw·d)对去卵巢引起的大鼠子宫萎缩有一定的抑制作用.SDG对去卵巢大鼠的肝脏、脾脏、双肾的胜体比均无显著影响(P>0.05),但能使大鼠的胸腺脏体比显著增加(P<0.05);显著升高血清高密度脂蛋白水平(P<0.05),对低密度脂蛋白没有影响(P>0.05),高剂量SDG可显著降低血清总胆固醇水平(P<0

  16. Effect of a 4-year workplace-based physical activity intervention program on the blood lipid profiles of participating employees: the high-risk and population strategy for occupational health promotion (HIPOP-OHP) study.

    Science.gov (United States)

    Naito, Mariko; Nakayama, Takeo; Okamura, Tomonori; Miura, Katsuyuki; Yanagita, Masahiko; Fujieda, Yoshiharu; Kinoshita, Fujihisa; Naito, Yoshihiko; Nakagawa, Hideaki; Tanaka, Taichiro; Ueshima, Hirotsugu

    2008-04-01

    Individuals who are physically fit or engage in regular physical activity have a lower incidence of cardiovascular disease and risk of mortality. We conducted a large-scale controlled trial of interventions to decrease cardiovascular risk factors, during which we assessed the effect of a workplace-based intervention program, which was part of a population strategy for promoting long-term increases in physical activity, on the blood lipid profiles of participating employees. Data were collected from 2929 participants and this report presents the results of a survey conducted in five factories for the intervention group and five factories for the control group at baseline and year 5. The absolute/proportional changes in HDL-cholesterol were 2.7 mg/dL (4.8%) in the intervention group and -0.6 mg/dL (-1.0%) in the control group. The differences between the two groups in the change in serum levels of HDL-cholesterol were highly significant (ppromoting physical activity raises serum HDL-cholesterol levels of middle-aged employees. Increased awareness of the benefits of physical activity, using environmental rearrangement and health promotion campaigns, which especially target walking, may have contributed to a beneficial change in serum HDL-cholesterol levels in the participants. PMID:17868680

  17. Analysis of blood sugar and lipid metabolism parameters and the prevalence of different metabolic disorder in two ethnicities%维汉民族健康体检人群糖、脂代谢紊乱情况分析

    Institute of Scientific and Technical Information of China (English)

    张伟; 王先敏; 蔡志华; 艾木拉江·买买提艾力; 孙玉萍

    2011-01-01

    the Diabetes mellitus were increased ( P<0.05 ) ,but the hypertriglyceridemia were higher in every age groups .Concluion The blood biochemistry targets were show different between sex and ethnic ,accorpany with the increasing of age groups ,the blood biochemistry targets were changed unfavorable to human health and the prevalence of different metabolic disorder were increased too ,so take corrsponding measures to control the level of blood sugar and lipid rmetabolism parameters and the prevalence of different metabolic disorder will have a positive impact on health .

  18. Effect of cobalt chloride on content of lipids and lipoproteins in serum and liver of rats.

    Science.gov (United States)

    Kaliman, P A; Shalamov, R V; Zagaiko, A L

    1997-07-01

    Lipids and the composition of lipoproteins in blood serum and liver cytosol, total lipid, and phospholipid contents in liver subcellular fractions and the spectrum of microsomal liver lipids were studied in male Wistar rats after a single injection of cobalt chloride. Virtually all lipid and lipoprotein fractions in blood and liver were increased and lipoprotein composition was changes. The lipid composition of liver microsomes did not change under these conditions. Thus, microsomal membranes are stable under developing oxidative stress. PMID:9331964

  19. 分级检验方法学在血脂生化检验中的应用%Application discussion of grading test methodology in the blood lipid biochemical test

    Institute of Scientific and Technical Information of China (English)

    陈桂明; 罗富银; 赵芳

    2015-01-01

    目的:探讨分级检验方法学在血脂生化检验中的应用效果。方法:收治体检人群97例,抽取患者空腹血液,分别采用传统拉网式检验和分级检验,对患者胆固醇、甘油三酯等血脂指标进行检查,并将结果进行比较。结果:分级检测的低密度脂蛋白固醇、载脂蛋白A、载脂蛋白B的阳性率明显高于拉网式检测(P<0.05)。97例病例中,有2例低密度脂蛋白胆固醇阳性漏检病例,漏检率2.1%。结论:分级检验准确性较高、操作简便,更适合应用在临床中。%Objective:To explore the application effect of grading test methodology in the blood lipid biochemical test.Methods:97 cases of physical examination people were selected.The fasting blood of patients were extracted.They were respectively given traditional trawl test and grading test.The cholesterol,triglycerides and other blood fat indexes of patients were examined.The results were compared.Results:The positive rates of low density lipoprotein cholesterol,apolipoprotein B and apolipoprotein B of grading test were significantly higher than those of the trawl test(P<0.05).In 97 cases,2 cases were low density lipoprotein cholesterol positive missing cases,and the missing rate was 2.1% .Conclusion:The grading test has higher accuracy,simple operation.It is more suitable for application in clinical.

  20. 燕麦对高胆固醇血症男性中老年人血脂水平的影响%Effect of Oat on Blood Lipids Level of Middle-aged and Elderly Men with hyPercholesterolemia

    Institute of Scientific and Technical Information of China (English)

    李丽祥; 张坚; 宋鹏坤; 王春荣; 满青青; 孟丽萍

    2011-01-01

    目的:观察食用燕麦对城市患高胆固醇血症男性中、老年人空腹血脂水平的影响.方法:参考《中国成人血脂异常防治指南》中的判断标准,将65名患高胆固醇血症男性中、老年人随机分为2组,分别为燕麦组(33人),每人每日给予100 g即冲即食燕麦片;对照组(32人),每人每日给予100 g普通挂面,试验周期6周.研究开始前和6周时对两组受试者进行基本健康状况问卷调查、人体测量、血生化检测以及膳食调查.结果:燕麦组和对照组的能量、蛋白质、脂肪、碳水化合物摄入量无显著差异,但燕麦组膳食纤维摄入量明显增加,增幅显著大于对照组,P<0.05.6周结束时,燕麦组血清总胆固醇(T℃)、低密度脂蛋白胆固醇(LDL-C)、载脂蛋白B(apoB)水平显著降低(P<0.05);血清TC、LDL-C平均降低幅度分别为7.0%和7.5%.对照组血清胆固醇、脂蛋白及载脂蛋白水平在试验期间未见显著变化.结论:对于我国城市中患高胆固醇血症的男性中、老年人,每日食用一定量的燕麦作为膳食中的粮谷类食物,可有效降低血清TC、LDL-C和apoB水平,减少心脑血管疾病发生的危险.%Objective:To observe the impact of oat intake on the blood lipid level of middle-aged and elderly men with hypercholesterolemia. Methods:By referring to the standards in the Chinese Guidelines on Prevention and Treatment of Dyslipidemia in Adults, 65 middle-aged and elderly men with hypercholesterolemia was selected and randomly assigned to consume 100 grams of instant oatmeal per day (Oat group,33 subjects) or receive 100 grams common noodle (Control,32 subjects) for 6 weeks. At the beginning and the final time of the intervention, basic health condition questionnaire survey, body measurement, blood biochemical testing and diet survey were undertaken. Results:There is no significant changes in energy, protein, fat, carbohydrate intake between the two groups while dietary

  1. Oral lipid-based nanoformulation of tafenoquine enhanced bioavailability and blood stage antimalarial efficacy and led to a reduction in human red blood cell loss in mice

    Directory of Open Access Journals (Sweden)

    Melariri P

    2015-02-01

    Full Text Available Paula Melariri,1 Lonji Kalombo,2 Patric Nkuna,2 Admire Dube,2,3 Rose Hayeshi,2 Benhards Ogutu,4,5 Liezl Gibhard,6 Carmen deKock,6 Peter Smith,6 Lubbe Wiesner,6 Hulda Swai2 1Polymers and Composites, Material Science and Manufacturing, Council for Scientific and Industrial Research, Port Elizabeth, South Africa; 2Polymer and Composites, Material Science and Manufacturing, Council for Scientific and Industrial Research, Pretoria, South Africa; 3School of Pharmacy, University of the Western Cape, Bellville, South Africa; 4Centre for Research in Therapeutic Sciences, Strathmore University, Nairobi, Kenya; 5Centre for Clinical Research, Kenya Medical Research Institute, Nairobi, Kenya; 6Division of Pharmacology, University of Cape Town Medical School, Groote Schuur Hospital, Cape Town, South Africa Abstract: Tafenoquine (TQ, a new synthetic analog of primaquine, has relatively poor bioavailability and associated toxicity in glucose-6-phosphate dehydrogenase (G6PD-deficient individuals. A microemulsion formulation of TQ (MTQ with sizes <20 nm improved the solubility of TQ and enhanced the oral bioavailability from 55% to 99% in healthy mice (area under the curve 0 to infinity: 11,368±1,232 and 23,842±872 min·µmol/L for reference TQ and MTQ, respectively. Average parasitemia in Plasmodium berghei-infected mice was four- to tenfold lower in the MTQ-treated group. In vitro antiplasmodial activities against chloroquine-sensitive and chloroquine-resistant strains of Plasmodium falciparum indicated no change in half maximal inhibitory concentration, suggesting that the microemulsion did not affect the inherent activity of TQ. In a humanized mouse model of G6PD deficiency, we observed reduction in toxicity of TQ as delivered by MTQ at low but efficacious concentrations of TQ. We hereby report an enhancement in the solubility, bioavailibility, and efficacy of TQ against blood stages of Plasmodium parasites without a corresponding increase in toxicity

  2. Blood Clots

    Science.gov (United States)

    ... Index A-Z Blood Clots Blood clots are semi-solid masses of blood that can be stationary (thrombosis) ... treated? What are blood clots? Blood clots are semi-solid masses of blood. Normally, blood flows freely through ...

  3. Effects of abnormal savda mature granules on blood lipid and blood pressure in renovascular hypertensive rats%异常黑胆质成熟剂颗粒对肾血管性高血压大鼠血压和血脂的影响

    Institute of Scientific and Technical Information of China (English)

    努尔曼·麦麦提; 阿衣努尔·买提斯迪克; 阿不都热依木·玉苏甫; 阿孜古丽·吐鲁洪

    2013-01-01

    Object:To investigate the effect of Abnormal Savda Munziq Granular on renovascular hypertensive rats (RHR) and explore the mechanism of Lowering the blood pressure and blood lipids and prevention and control and treatment of left ventricular hypertrophy.Methods:Select 72 male Sprague-Dawley rats were randomly assigned to 6 groups as follows:normal control group,sham operation group,model group,Abnormal Savda Munziq Granular of low dose group,Abnormal Savda Munziq granular of medium dose group,Abnormal Savda Munziq granular of high dose group.The mice continuously given drugs for 10 weeks.The blood pressure were measured by Non-invasive tail artery cuff method,measure the serum triglyceride (TG),Cholesterol (TC),High-density lipoprotein (HDL-C),Low-density lipoprotein (HDL-C),Left ventricular mass index (LVMI) etc; Results:Abnormal Savda Munziq granule various dosage groups can reduce the rats'blood pressure,Model group and other groups have significant difference (P < 0.05),can be reduced rat blood pressure,TG、TC、LVMI etc; Conclusion:Abnormal Savda Munziq granule have antihypertensive effects in spontaneously hypertensive rats,control blood lipid levels and hypertension caused by left ventricular hypertrophy have a preventive effect.%目的:初步探讨异常黑胆质成熟剂颗粒(以下简称异黑成熟颗粒)对肾血管性高血压大鼠的降压、降低血脂和防治左心室肥厚的作用机制.方法:选择SD雄性大鼠72只随机分为正常对照组、假手术组、模型组、异常黑胆质成熟剂低剂量组、中剂量组、高剂量组6组,干预持续10周.采用无创尾动脉套袖法观察异常黑胆质成熟颗粒对高血压大鼠的血压、血清甘油三酯(TG)、胆固醇(TC)、高密度脂蛋白HDL-C、低密度脂蛋白LDL-C、左心室质量指数(LVMI)等指标.结果:异常黑胆质成熟剂颗粒各剂量组均可降低大鼠血压,模型组与其他组比较差异有统计学意义(P<0.05),可降

  4. Evaluation of blood chemical, lipids profile and immune response on broiler chicks fed with milk thistle (Silybum mari- anum L. and thyme (Thymus vulgaris L. seeds in south-eastern Iran

    Directory of Open Access Journals (Sweden)

    Hossein Ansari Nik

    2014-11-01

    Full Text Available This study was conducted to investigate the effects of supplementation of milk thistle seeds (MTS and thyme seeds (TS on blood chemical, lipid profile and immune response in broiler chicks. In this study, 160 one-day-old chicks (Ross 308 were allocated to four treatments with four replicates based on a completely randomized design in a 2×2 factorial arrangement. The treatment groups were (A basal control diet, (B basal diet with 0.2 g/kg of MTS, (C basal diet with 0.2 g/kg of TS and (D basal diet with 0.2 g/kg of MTS and 0.2 g/kg of TS. Birds fed the (D supplemented treatment (MTS plus TS had the greatest levels of total protein, 4.26±0.27 g/L; albumin, 2.21±0.02 g/L; globulin 2.28±0.23 g/L; and aspartate aminotransferase (AST activity, 152.18±4.46 U/L than the control birds [group (A] (P<0.05. MTS alone or in combination with TS reduced the cholesterol in the serum of the broilers (P<0.05, and this effect was more pronounced for the (C treatment (TS alone (P<0.05. Treatment consumption with MTS plus TS [group (D] significantly increased the concentration of high-density lipoprotein cholesterol (HDL-C, 90.32±2.28 mmol/L, but low-density lipoprotein cholesterol (LDL-C, 10.44±0.07 mmol/L, and triglyceride concentrations, 60.75±2.65 mmol/L, were decreased compared to control (P<0.05. However, none of the immunity parameters and liver enzymes differed significantly in MTS or TS groups. The present research indicated that supplements of MTS and TS have a protective influence on the lipids profile, total protein, globulin, albumin and AST levels in broiler chicks.

  5. 桑叶总黄酮对糖尿病高脂血症大鼠血糖、血脂和胸主动脉p22phox mRNA表达的影响%Effects of Total Flavonoids from Mulberry Leaves on Blood Glucose, Blood Lipid and the expression of P22phox mRNA in Thoracic Aorta in Diabetic and Hyperlipidemia Rats

    Institute of Scientific and Technical Information of China (English)

    张凇铭; 黄平; 杜静静

    2014-01-01

    目的:探讨桑叶总黄酮(FML)对糖尿病高脂血症大鼠血糖、血脂和胸主动脉p22phox mRNA表达的影响。方法60只雄性SD大鼠随机分成空白组、模型组、FML低、中、高剂量组和西药组,每组10只,观察FML对大鼠血糖、血脂、胸主动脉p22phox mRNA表达的影响。结果与空白组比较,模型组大鼠血糖、血脂显著升高(P<0.01),胸主动脉p22phox mRNA表达明显上调(P<0.01)。与模型组比较,FML中、高剂量组及西药组大鼠血糖、血脂降低(P<0.05或P<0.01),FML中、高剂量组及西药组大鼠胸主动脉p22phox mRNA表达明显下调(P<0.05或P<0.01)。结论 FML显著降低糖尿病高脂血症大鼠血糖、血脂水平,其机制可能与FML下调大鼠胸主动脉p22phox mRNA表达,进而抑制NADPH氧化酶活化有关。%Objective To investigate the influence of total flavonoids from mulberry leaves (FML) on blood glu-cose, blood lipid and the expression of p22phox mRNA in thoracic aorta in rats with diabetes and hyperlipidemia. Methods Diabetes and hyperlipidemia rats were used in this study. Sixty SD male rats were randomly into blank group, model group, treatment groups (high, middle, low dose of FML and western medicine group), 10 in each group. The effect of FML on blood glucose, blood lipid and the expression of p22phox mRNA in thoracic aorta was observed. Results Blood glucose, blood lipid and the expression of p22phox mRNA in thoracic aorta in rats in model group were higher than those in blank group(P<0.01). Compared with model group, rats in middle and high dose of FML groups and western medicine group had lower blood glucose, blood lipid and decreased expression of p22phox mRNA in thoracic aorta (P<0.05 or P<0.01). Conclusion FML can inhibit levels of blood glucose and blood lipids in diabetes and hyperlipidemia rats. This effect may be due to decreasing the expression of p22phox mRNA in thoracic aorta and then the activation of

  6. Efeitos da isoflavona sobre os sintomas climatéricos e o perfil lipídico na mulher em menopausa Effects of isoflavone on menopausal symptoms and blood lipids in postmenopausal women

    Directory of Open Access Journals (Sweden)

    Eliana Aguiar Petri Nahás

    2003-06-01

    Full Text Available OBJETIVO: avaliar os efeitos da isoflavona, do gérmen da soja, sobre os sintomas climatéricos e o perfil lipídico na mulher em menopausa. MÉTODOS: foi conduzido estudo prospectivo, com 50 mulheres em menopausa, divididas em: G1, usuárias de isoflavona (60 mg/dia (n=25, e G2, placebo (n=25. Os critérios de inclusão foram FSH >40 mUI/mL e presença de fogachos. Foram excluídas as vegetarianas, fumantes, asiáticas, portadoras de doenças gastrointestinais e usuárias de terapia de reposição hormonal. No seguimento, de seis meses, foram obtidos o índice menopausal de Kupperman (IMK, o perfil hormonal e o lipídico. Na análise estatística, empregaram-se ANOVA, o teste t pareado e as provas não paramétricas de Wilcoxon e Mann-Whitney. RESULTADOS: os valores medianos do IMK, inicialmente iguais entre os grupos (IMK = 20, reduziram-se nas usuárias de isoflavona aos 2 e 4 meses (IMK = 14 e 9, respectivamente e no grupo controle, apenas aos 2 meses (IMK = 15 (pPURPOSE: to evaluate the effects of soy germ isoflavone on menopausal symptoms and blood lipids in postmenopausal women. METHODS: a prospective study was performed on 50 women, randomly divided into two groups: 25 women on soy germ isoflavones (60 mg/day, capsules (G1 and 25 women on placebo (G2. Inclusion criteria: women with hot flushes and FSH >40 mIU/mL, non-vegetarian, non-smoker, non-Asiatic, not in use of hormone replacement therapy and without disease of the gastrointestinal tract. For six months, the menopausal Kupperman index (MKI and hormonal and lipid profiles were assessed. For statistical analysis, ANOVA, t test and the non-parametric Kruskal-Wallis and Mann-Whitney tests were used. RESULTS: the median MKI values, initially similar in both groups, decreased in G1 at two and four months (MKI = 14 and 9, respectively, and in G2 at two months (MKI = 15 (p<0.01. At six months, isoflavone was significantly superior to placebo in reducing hot flushes (44 versus 12

  7. 化瘀温胆汤对代谢综合征大鼠血脂、血压及脂肪细胞因子的干预作用%Effects of Huayu Wendan Decoction on Blood Pressure, Blood lipid and Adipose Cell Cytokine of Metabolic Sydrome Rats

    Institute of Scientific and Technical Information of China (English)

    张福利; 曹昧; 马伯艳; 张洋; 宋琳

    2011-01-01

    目的:探讨化瘀温胆汤对代谢综合征大鼠血压、血脂异常的治疗作用及部分机制.方法:健康雄性SD大鼠高温高湿环境下喂饲高脂高糖高盐饮食12周,复制大鼠代谢综合征模型.通过检测血压、血清胆固醇(TC)、甘油三酯(TG)、低密度脂蛋白(LDL-C)、高密度脂蛋白(HDL -C),探讨化瘀温胆汤灌胃给药4周后对模型大鼠血压、血脂异常的改善作用.检测脂联素、瘦素、肿瘤坏死因子-α(TNF -α)、白介素-6(IL -6)变化情况,探讨该方治疗代谢综合征的部分机制.结果:化瘀温胆汤可明显降低模型大鼠的血清TC、TG、LDL -C水平,升高HDL -C,与模型对照组比较有显著性差异(P<0.01);可降低模型大鼠血压,与模型对照组比较有显著性差异(P<0.01).化瘀温胆汤可使脂联素水平明显升高,与模型对照组比较有显著性差异(P<0.01);瘦素水平、TNF -α、IL -6水平均有不同程度的降低,与模型对照组比较均具有显著性差异(P<0.01).结论:化瘀温胆汤对代谢综合征模型大鼠血压、血脂异常有较好的疗效,其机制可能与其调节脂联素、瘦素、TNF -o、IL -6水平有关.%Objectives: To observe the therapeutic effects of HuaYu Wendan decoction on abnormal blood pressure and blood lipids of laboratory metabolic syndrome (MS) rats and its partial mechanism. Methods; To render rats by feeding high - lipid, high - glucose, high - salt diet in the high temperature and high humidity environment and then treated them with HuaYu Wendan decoction for four weeks. Then we observed the effects of the decoction on blood pressure, serum cholesterol, triglyceride, low density lipoprotein and high density lipoprotein after four weeks'administration. We studied the mechanism through detecting several adipose cell cytokines such as adiponectin, leptin, tumor necrosis factor -alpha (TNF -a) and interleukin 6(IL - 6 ) . Results: Huayu Wendan decoction had functions of decreasing

  8. The Association Between Antihypertensive Medication Nonadherence and Visit-to-Visit Variability of Blood Pressure: Findings From the Antihypertensive and Lipid-Lowering Treatment to Prevent Heart Attack Trial.

    Science.gov (United States)

    Kronish, Ian M; Lynch, Amy I; Oparil, Suzanne; Whittle, Jeff; Davis, Barry R; Simpson, Lara M; Krousel-Wood, Marie; Cushman, William C; Chang, Tara I; Muntner, Paul

    2016-07-01

    Low adherence to antihypertensive medication has been hypothesized to increase visit-to-visit variability (VVV) of blood pressure (BP). We assessed the association between antihypertensive medication adherence and VVV of BP in the Antihypertensive and Lipid-Lowering Treatment to Prevent Heart Attack Trial (ALLHAT). VVV of BP was calculated using SD independent of mean, SD, and average real variability across study visits conducted 6 to 28 months after randomization. Participants who reported taking <80% of their antihypertensive medication at ≥1 study visits were categorized as nonadherent. Participants were followed up for cardiovascular events and mortality after the assessment of adherence and VVV of BP. SD independent of mean of BP was higher for nonadherent (n=2912) versus adherent (n=16 878) participants; 11.4±4.9 versus 10.5±4.5 for systolic BP; 6.8±2.8 versus 6.2±2.6 for diastolic BP (each P<0.001). SD independent of mean of BP remained higher among nonadherent than among adherent participants after multivariable adjustment (0.8 [95% confidence interval, 0.7-1.0] higher for systolic BP and 0.4 [95% confidence interval, 0.3-0.5] higher for diastolic BP]. SD and average real variability of systolic BP and diastolic BP were also higher among nonadherent than among adherent participants. Adjustment for nonadherence did not explain the association of VVV of BP with higher fatal coronary heart disease or nonfatal myocardial infarction, stroke, heart failure, or mortality risk. In conclusion, improving medication adherence may lower VVV of BP. However, VVV of BP is associated with cardiovascular outcomes independent of medication adherence. PMID:27217410

  9. Relationship between occupational stressors and blood lipid in thermal power plant workers%热电厂工人职业紧张相关因素与血脂的关系

    Institute of Scientific and Technical Information of China (English)

    吴辉; 余善法; 周文慧; 谷桂珍; 吴艳延

    2012-01-01

    目的 探讨职业紧张相关因素与血脂的关系.方法 用整群抽样的方法对某热电厂498名在职工人进行横断面调查,有效样本446份;用问卷法测评职业紧张因素、个性特征、紧张缓解因素和紧张结局等;常规法测定血清中总胆固醇和甘油三酯的浓度.结果 性别、年龄、婚姻状况、文化程度、吸烟和饮酒等人口统计学特征及健康相关行为的不同水平组间总胆固醇比较,差异无统计学意义(P>0.05);甘油三酯在年龄和工龄方面差异具有统计学意义(P<0.05).工作前景未知性高、低水平组间的总胆固醇水平差异有统计学意义(P<0.05),高分组的总胆固醇水平高于低分组;控制混杂因素后,内在付出高、低水平组间的甘油三酯水平差异有统计学意义(P<0.05),高分组的甘油三酯水平高于低分组.多因素非条件logistic回归分析,以总胆固醇为应变量,40~50岁年龄组与工作组织满意感进入方程;前者与总胆固醇呈正向关系(OR=2.655,P<0.05),后者与总胆固醇呈负向关系(OR=0.906,P<0.05).在甘油三酯为应变量的回归方程中,饮酒和负性情绪进入方程,均与甘油三酯呈正向关系(OR=2.771,P<0.01;OR=1.297,P<0.01).结论 职业紧张相关因素与血脂水平之间存在相关性,职业紧张可导致血脂水平的升高.%Objective To explore the relationship between occupational stressors and blood lipid. Methods A cross-sectional epidemiologicai study was conducted on 498 workers from a thermal power plant, 446 workers returned qualified questionnaires. The occupational stressors* personalities, relieving factors, the outcomes of stress and other related factors were measured by questionnaires. Serum total cholesterol and triglyceride in all samples were detected by biochemistry methods. Results There was no significant for cholesterol in different levels of sex, age, the length of service and other demographics and

  10. EFFECTS OF γ-AMINOBUTYRIC ACID ON BLOOD LIPIDS IN RATS UNDER EMOTIONAL STRESS%γ-氨基丁酸对情绪应激大鼠血脂的影响

    Institute of Scientific and Technical Information of China (English)

    欧阳俊彦; 胡卓炎; 褚玥; 梁添; 赖玉婷; 枷春红

    2013-01-01

    目的 探讨γ-氨基丁酸(GABA)对情绪应激大鼠血脂代谢的调节作用.方法 利用不确定性空瓶刺激的方法建立焦虑应激大鼠模型,将40只雄性SD大鼠随机分为对照组、模型组、GABA 0.5 (L)、1.0(M)和2.0 mg/kg(H)组,应激前分别给予大鼠灌服生理盐水或相应剂量的GABA,14d后采用高架十字迷宫实验(EPM)检测大鼠行为,然后采集血样,测定大鼠血浆甘油三酯(TG)、总胆固醇(TC)、低密度脂蛋白胆固醇(LDL-C)和高密度脂蛋白胆固醇(HDL-C)水平,并计算非高密度脂蛋白胆固醇(non-HDL-C)含量、TG/HDL-C、LDL-C/HDL-C比值.结果:应激后对照组体重和食物利用率极显著高于其余各组(P<0.01);模型组进入开放臂的时间百分比极显著低于对照组和GABA 2 mg/kg组(P<0.01),进入开放臂的次数百分比则显著低于对照组(P<0.05);各组大鼠的入臂总次数差异不显著(P>0.05).血脂水平分析显示模型组血浆中TC含量显著高于GABA 2 mg/kg组(P<0.05),non-HDL-C含量则显著高于对照组(P<0.05),极显著高于GABA 2 mg/kg组(P<0.01),模型组的LDL-C/HDL-C比值显著高于对照组和GABA 2 mg/kg组(P<0.05). 结论 2 mg/kg GABA能明显增加EPM中进入开放臂的时间百分比,降低TC与non-HDL-C含量并下调LDL-C/HDL-C比值,有效缓解焦虑情绪并改善血脂代谢.%Objective To observe the modulation effects of γ-aminobutyric acid (GABA) on blood lipids in rats under emotional stress.Methods Forty male Sprague-Dawley rats were divided into 5 groups randomly (n=8 each):control group,model group,GABA 0.5,1.0 and 2.0 mg/kg group.Animal model of emotional stress was induced by empty water bottles stimulation method.The rats were administered with saline or different doses of GABA orally before they suffered from emotional stress.14 d later,animal behaviors were assessed in the elevated plus maze test (EPM),and plasma levels of triglyceride (TG),total cholesterol (TC),low density

  11. Lewis phenotype, secretor status, and coeliac disease.

    OpenAIRE

    Dickey, W; Wylie, J D; Collins, J S; Porter, K G; Watson, R G; McLoughlin, J C

    1994-01-01

    Patients who cannot secrete ABO and Lewis blood group antigens into body fluids, an ability controlled by a single gene on chromosome 19, are known to be at increased risk of certain autoimmune diseases associated with human leucocyte antigen (HLA) markers. This study investigated the possibility of an association with coeliac disease using red cell Lewis (Le) blood group phenotype to infer secretor status. Among 73 patients with coeliac disease who had Le a or b antigen, 48% were non-secreto...

  12. EFFECTS OF DIETARY LIPID LEVELS ON GROWTH PERFORMANCE, NUTRIENT DIGESTIBILITY AND BLOOD BIOCHEMICAL INDICES OF GIFT TILAPIA (OREOCHROMIS NILOTICUS)%饲料脂肪水平对吉富罗非鱼生产性能、营养物质消化及血液生化指标的影响

    Institute of Scientific and Technical Information of China (English)

    王爱民; 韩光明; 封功能; 杨文平; 郭家豪; 王恬; 徐跑

    2011-01-01

    In order to determine the optimal levels of the lipid of GIFT strain Nile tilapia (Oreochromis niloticus), 630 GIFT with average weight of (2.63±0. 16) g were divided into six groups (1.73% lipid, 3.71% lipid, 5.69% lipid, 7.67% lipid, 9.64% lipid and 16.55% lipid) randomly. One with triplication was the control group fed with basal diet (1.73% lipid), the others were the treated groups fed with basal diet (1.73% lipid) supplemented with 2%, 4%, 6%, 8% and 15% fish oil, respectively. After rearing 90d, the growth, feed conversion ratio, nutrient apparent digestibility and blood biochemical parameter were determined. Results showed that, along with dietary lipid level raising, weight gain rate and specific growth rate had a trend of going up first then falling down (P<0.05), protein efficiency ratio improved (P<0.01) and feed conversion ratio reduced (P<0.01). Second-order regression of weight gain rate on concentrations of dietary lipid indicated that the optimal dietary lipid for maximal growth of GIFT tilapia was about 9.34%. Crude protein and dry-matter digestibility had no significant effected by dietary lipid levels (P>0.05). Crude lipid and phosphorus digestibility raised significantly with dietary lipid level increasing (P<0.05). Albumin and albumin/globulin ratio in 1.73% group (this group did not added fish oil) was significantly higher than other group (P<0.05). Along with dietary lipid level raising, cholesterol and alkaline phosphatase of fish serum raised significantly (P<0.01). Dietary lipid level had significantly influence on blood glucose (P<0.05), and there was no significantly influence on the content of triglyceride and the activities of glutamic-pyruvic transaminase and glutamic-oxaloacetic transaminase (P>0.05). It indicated that the level of lipid in the dietary could improve the growth, the apparent digestibilities of crude lipid and phosphorus to some degree, but excessive amount of lipid

  13. Prediction of disease and phenotype associations from genome-wide association studies.

    Directory of Open Access Journals (Sweden)

    Stephanie N Lewis

    Full Text Available BACKGROUND: Genome wide association studies (GWAS have proven useful as a method for identifying genetic variations associated with diseases. In this study, we analyzed GWAS data for 61 diseases and phenotypes to elucidate common associations based on single nucleotide polymorphisms (SNP. The study was an expansion on a previous study on identifying disease associations via data from a single GWAS on seven diseases. METHODOLOGY/PRINCIPAL FINDINGS: Adjustments to the originally reported study included expansion of the SNP dataset using Linkage Disequilibrium (LD and refinement of the four levels of analysis to encompass SNP, SNP block, gene, and pathway level comparisons. A pair-wise comparison between diseases and phenotypes was performed at each level and the Jaccard similarity index was used to measure the degree of association between two diseases/phenotypes. Disease relatedness networks (DRNs were used to visualize our results. We saw predominant relatedness between Multiple Sclerosis, type 1 diabetes, and rheumatoid arthritis for the first three levels of analysis. Expected relatedness was also seen between lipid- and blood-related traits. CONCLUSIONS/SIGNIFICANCE: The predominant associations between Multiple Sclerosis, type 1 diabetes, and rheumatoid arthritis can be validated by clinical studies. The diseases have been proposed to share a systemic inflammation phenotype that can result in progression of additional diseases in patients with one of these three diseases. We also noticed unexpected relationships between metabolic and neurological diseases at the pathway comparison level. The less significant relationships found between diseases require a more detailed literature review to determine validity of the predictions. The results from this study serve as a first step towards a better understanding of seemingly unrelated diseases and phenotypes with similar symptoms or modes of treatment.

  14. An Adaptive Association Test for Multiple Phenotypes with GWAS Summary Statistics.

    Science.gov (United States)

    Kim, Junghi; Bai, Yun; Pan, Wei

    2015-12-01

    We study the problem of testing for single marker-multiple phenotype associations based on genome-wide association study (GWAS) summary statistics without access to individual-level genotype and phenotype data. For most published GWASs, because obtaining summary data is substantially easier than accessing individual-level phenotype and genotype data, while often multiple correlated traits have been collected, the problem studied here has become increasingly important. We propose a powerful adaptive test and compare its performance with some existing tests. We illustrate its applications to analyses of a meta-analyzed GWAS dataset with three blood lipid traits and another with sex-stratified anthropometric traits, and further demonstrate its potential power gain over some existing methods through realistic simulation studies. We start from the situation with only one set of (possibly meta-analyzed) genome-wide summary statistics, then extend the method to meta-analysis of multiple sets of genome-wide summary statistics, each from one GWAS. We expect the proposed test to be useful in practice as more powerful than or complementary to existing methods. PMID:26493956

  15. ICU护士工作压力源、疲倦感及血压、血糖和血脂的变化特点%Characteristics of Job Stressors, Fatigue and Change of Blood Pressure, Blood Glucose and Lipid among ICU Nurses

    Institute of Scientific and Technical Information of China (English)

    李丹; 黄津芳; 汤黎黎; 王剑; 贾红春

    2012-01-01

    目的 探讨ICU护士工作压力源、疲倦感及血压、血糖和血脂的变化特点.方法 选择参加2011年春季体检的沈阳军区总医院和解放军第463医院的临床护理人员84名为研究对象,其中ICU护士42名,门诊护士42名.采用现场1∶1配对方法(配对条件为:相同年龄、护龄、目前岗位工作时间、性别、职称及文化程度),比较两组护士护士工作压力源评分、Maslach工作疲倦感量表(Maslach burnout inventory,MBI)评分情况,并分析其血压、血糖、血脂等项体检结果.结果 ICU护士“护士工作压力源”量表各子条目评分及总分均明显高于门诊护士(P<0.01或P<0.05).ICU护士的情绪枯竭、去人格化倾向等维度评分均明显高于门诊护士,而个人工作成就感维度分则显著低于后者(P<0.01).ICU组护士的收缩压和舒张压均值均明显高于门诊护士(P<0.05).且其空腹血糖(fasting plasma glucose,FPG)、餐后2h血糖(2 h postprudial plasma glucose,2hPG)和三酰甘油(triglyceride,TG)水平均明显高于门诊护士,而血清高密度脂蛋白胆固醇(high-density lipoprotein cholesterol,HDL-C)水平则低于后者(P<0.05).结论 门诊护理工作人员护理工作量及劳动强度相对较小,而大医院ICU从业护士工作压力源较大,疲倦感表现突出,存在着血压、血糖和血脂增高的趋向.%Objective To analyze the characteristics of job stressors and fatigue and its influence on blood pressure, blood glucose and lipid among ICU nurses. Methods Eighty-four clinical nurses who participated in the physical examination of the two hospitals in the spring of 2011 were enrolled in the research,including 42 ICU nurses and 42 outpatient nurses. Using on-site 1:1 pairing method (depending on the same age,nurse-job seniority, working time in current position,sex,professional title,and educational level) ,the scores of nurse job stressors questionnaire and Maslach burnout inventory (MBI) were

  16. 桂皮提取物对糖尿病大鼠血糖和血脂含量的影响%Effects of cinnamon on blood sugar and lipid levels in diabetic rats

    Institute of Scientific and Technical Information of China (English)

    陈常秀; 李永洙

    2011-01-01

    【Objective】 The study was conducted on the effects of cinnamon on blood sugar and lipid levels in diabetic rats.【Method】 The diabetic rats were induced by intraperitoneal injection of 200 mg/kg alloxan to establish diabetic model.The rats were divided into control group(Ⅰ),model group(Ⅱ),low dose cinnamon treating group(Ⅲ) and high dose cinnamon treating group(Ⅳ).The diabetic rats of group Ⅲ and Ⅳ were orally administered with 100 and 200 mg/(kg·d) cinnamon extract respectively,for 30 days.The rats of group Ⅰ and Ⅱ were orally administered with saline respectively by the same way and the same dose.The daily feed consume,water intake and urine excretion were recorded.The blood sugar,TC,TG,LDL-C,HDL-C level and the body weight were estimated at 0 and 30 d.【Result】 Compared with the rats of group Ⅰ,the body weight of all the diabetic rats of group(Ⅱ,Ⅲ and Ⅳ) decreased significantly(P0.05),and the blood sugar level increased significantly(P0.05) at 0 d.Compared with the rats of groupⅡ,the blood sugar,TC,TG,LDL-C level of the diabetic rats in group Ⅳdecreased significantly(P0.05),the body weight and HDL-C level increased significantly(P0.05) at 30 d.Compared with the rats of group Ⅰ,there were no differences in body weight,blood sugar,TC,TG,LDL-C and HDL-C level of the diabetic rats in group Ⅳ at 30 d(P0.05).【Conclusion】 The cinnamon extract(200 mg/(kg ·d)) decreased the blood sugar,TC,TG and LDL-C level of the diabetic rats induced by alloxan,increased the body weight and enhanced HDL-C level.Enrichment of cinnamon with cinnamate can provide a functional food spice that could potentially benefit human health.%【目的】探讨桂皮提取物对糖尿病大鼠血糖和血脂含量的影响。【方法】给大鼠腹腔注射200mg/kg的四氧嘧啶建立糖尿病模型。试验共分4组:正常对照组(Ⅰ)、糖尿病模型组(Ⅱ)、低剂量桂皮提取

  17. Engineering lipid overproduction in the oleaginous yeast Yarrowia lipolytica.

    Science.gov (United States)

    Qiao, Kangjian; Imam Abidi, Syed Hussain; Liu, Hongjuan; Zhang, Haoran; Chakraborty, Sagar; Watson, Nicki; Kumaran Ajikumar, Parayil; Stephanopoulos, Gregory

    2015-05-01

    Conversion of carbohydrates to lipids at high yield and productivity is essential for cost-effective production of renewable biodiesel. Although some microorganisms can convert sugars to oils, conversion yields and rates are typically low due primarily to allosteric inhibition of the lipid biosynthetic pathway by saturated fatty acids. By reverse engineering the mammalian cellular obese phenotypes, we identified the delta-9 stearoyl-CoA desaturase (SCD) as a rate limiting step and target for the metabolic engineering of the lipid synthesis pathway in Yarrowia lipolytica. Simultaneous overexpression of SCD, Acetyl-CoA carboxylase (ACC1), and Diacylglyceride acyl-transferase (DGA1) in Y. lipolytica yielded an engineered strain exhibiting highly desirable phenotypes of fast cell growth and lipid overproduction including high carbon to lipid conversion yield (84.7% of theoretical maximal yield), high lipid titers (~55g/L), enhanced tolerance to glucose and cellulose-derived sugars. Moreover, the engineered strain featured a three-fold growth advantage over the wild type strain. As a result, a maximal lipid productivity of ~1g/L/h is obtained during the stationary phase. Furthermore, we showed that the engineered yeast required cytoskeleton remodeling in eliciting the obesity phenotype. Altogether, our work describes the development of a microbial catalyst with the highest reported lipid yield, titer and productivity to date. This is an important step towards the development of an efficient and cost-effective process for biodiesel production from renewable resources. PMID:25732624

  18. Lipid peroxides level in the Indonesian elderly

    Directory of Open Access Journals (Sweden)

    Purwantyastuti Purwantyastuti

    2005-06-01

    Full Text Available A cross-sectional study was done to see the possible association of plasma lipid peroxides in the elderly with age and other factors. Plasma lipid peroxides is a product of free radical reactions which according to the latest theory of aging is the cause of aging process. Lipid peroxides were also found high in coronary heart disease. Four hundred forty relatively healthy elderly, age 55-85 years, were randomly chosen from free living elderly under guidance of health care centers (PUSKESMAS in Jakarta. Anamnesis and physical examination were done in the morning in the health centers. Blood samples were taken in fasting conditions, plasma lipids and lipid peroxides were measured according to standard methods. There was an age difference of lipid peroxides level in the elderly, which increased with age up to 70 years old. Elderly 70 years old and over had low plasma lipid peroxides. The level was not related to high plasma lipids. Higher level was found when more chronic degenerative diseases were found. (Med J Indones 2005; 14: 71-7Keywords: lipid peroxides, aging

  19. Steroidal Compounds in Commercial Parenteral Lipid Emulsions

    Directory of Open Access Journals (Sweden)

    Rafat A. Siddiqui

    2012-08-01

    Full Text Available Parenteral nutrition lipid emulsions made from various plant oils contain steroidal compounds, called phytosterols. During parenteral administration of lipid emulsions, phytosterols can reach levels in the blood that are many fold higher than during enteral administration. The elevated phytosterol levels have been associated with the development of liver dysfunction and the rare development of liver failure. There is limited information available in the literature related to phytosterol concentrations in lipid emulsions. The objective of the current study was to validate an assay for steroidal compounds found in lipid emulsions and to compare their concentrations in the most commonly used parenteral nutrition lipid emulsions: Liposyn® II, Liposyn® III, Lipofundin® MCT, Lipofundin® N, Structolipid®, Intralipid®, Ivelip® and ClinOleic®. Our data demonstrates that concentrations of the various steroidal compounds varied greatly between the eight lipid emulsions, with the olive oil-based lipid emulsion containing the lowest levels of phytosterols and cholesterol, and the highest concentration of squalene. The clinical impression of greater incidences of liver dysfunction with soybean versus MCT/LCT and olive/soy lipid emulsions may be reflective of the levels of phytosterols in these emulsions. This information may help guide future studies and clinical care of patients with lipid emulsion-associated liver dysfunction.

  20. PAT proteins, an ancient family of lipid droplet proteins that regulate cellular lipid stores.

    Science.gov (United States)

    Bickel, Perry E; Tansey, John T; Welte, Michael A

    2009-06-01

    The PAT family of lipid droplet proteins includes 5 members in mammals: perilipin, adipose differentiation-related protein (ADRP), tail-interacting protein of 47 kDa (TIP47), S3-12, and OXPAT. Members of this family are also present in evolutionarily distant organisms, including insects, slime molds and fungi. All PAT proteins share sequence similarity and the ability to bind intracellular lipid droplets, either constitutively or in response to metabolic stimuli, such as increased lipid flux into or out of lipid droplets. Positioned at the lipid droplet surface, PAT proteins manage access of other proteins (lipases) to the lipid esters within the lipid droplet core and can interact with cellular machinery important for lipid droplet biogenesis. Genetic variations in the gene for the best-characterized of the mammalian PAT proteins, perilipin, have been associated with metabolic phenotypes, including type 2 diabetes mellitus and obesity. In this review, we discuss how the PAT proteins regulate cellular lipid metabolism both in mammals and in model organisms. PMID:19375517

  1. Steroidal Compounds in Commercial Parenteral Lipid Emulsions

    OpenAIRE

    Siddiqui, Rafat A; Zaloga, Gary P; Mary Hise; Guy Dutot; Thomas Pavlina; Zhidong Xu; Harvey, Kevin A.

    2012-01-01

    Parenteral nutrition lipid emulsions made from various plant oils contain steroidal compounds, called phytosterols. During parenteral administration of lipid emulsions, phytosterols can reach levels in the blood that are many fold higher than during enteral administration. The elevated phytosterol levels have been associated with the development of liver dysfunction and the rare development of liver failure. There is limited information available in the literature related to phytosterol conce...

  2. Lymphatic Lipid Transport: Sewer or Subway?

    OpenAIRE

    Dixon, J. Brandon

    2010-01-01

    The lymphatics began receiving attention in the scientific community as early as 1622, when Gasparo Aselli noted the appearance of milky white vessels in the mesentery of a well-fed dog. Since this time, the lymphatic system has been historically regarded as the sewer of the vasculature, passively draining fluid and proteins from the interstitial spaces (along with lipid from the gut) into the blood. Recent reports, however, suggest that the lymphatic role in lipid transport is an active and ...

  3. Lipid14: The Amber Lipid Force Field

    OpenAIRE

    Dickson, Callum J.; Madej, Benjamin D.; Skjevik, Åge A.; Betz, Robin M.; Teigen, Knut; Gould, Ian R.; Walker, Ross C.

    2014-01-01

    The AMBER lipid force field has been updated to create Lipid14, allowing tensionless simulation of a number of lipid types with the AMBER MD package. The modular nature of this force field allows numerous combinations of head and tail groups to create different lipid types, enabling the easy insertion of new lipid species. The Lennard-Jones and torsion parameters of both the head and tail groups have been revised and updated partial charges calculated. The force field has been validated by si...

  4. 内源性n-3多不饱和脂肪酸对fat-1转基因小鼠血糖血脂的影响%Effects of Endogenous n-3 Polyunsaturated Fatty Acids (PUFAs) on Blood Glucose and Serum Lipid in fat-1 Transgenic Mouse Model

    Institute of Scientific and Technical Information of China (English)

    周飞; 张晓宏; 邹祖全; 张才乔

    2015-01-01

    利用fat-1转基因小鼠模型,研究内源性n-3多不饱和脂肪酸(n-3 PUFAs)的血糖血脂调节作用.采用fat-1转基因小鼠和C57BL/6野生型小鼠喂食高n-6、低n-3 PUFAs的标准配方饲料4周,然后2组小鼠给予高糖饮液自由饮用4周,每周测体重.第8周末,取血离心测血糖、胰岛素、甘油三脂(TG)、胆固醇(TC)、高密度脂蛋白(HDL-C)、低密度脂蛋白(LDL-C)水平.结果表明:fat-1转基因小鼠体重增加幅度、空腹血糖值、血清胰岛素、胰岛素抵抗指数、血清TC、TG、HDL-C、LDL-C水平明显低于野生型小鼠(P<0.05).认为n-3 PUFAs能抑制体重增长,降低小鼠血糖、血脂和胰岛素抵抗,起到调节血糖、血脂的作用.%In this study, we investigate the effects of endogenous n-3 PUFAs on blood glucose and serum lipid in fat-1 transgenic mouse model. The transgenic and wild type mice are maintained on a 10%corn oil diet which has high ratio of n-6/n-3 polyunsaturated fatty acids for 4 weeks. Then make them drink high sugar pinocytosis freely for another four weeks. On the eighth weekend, serum glucose, insulin, serum triglyceride (TG), serum total cholesterol (TC), serum high-density lipoprotein cholesterol (HDL-C), serum low-density lipoprotein cholesterol (LDL-C) levels of blood serum are tested after blood collection and centrifugation. The weight gain rate, fasting blood sugar and serum insulin levels, insulin resistance index, serum TC, TG, HDL-C, LDL-C of fat-1 transgenic mice are found to be significantly lower than those of wild type mice (P<0.05). The tests indicate that n-3 polyunsaturated fatty acids can reduce the blood glucose and serum lipid, which may play a vital role in regulating blood lipid and decreasing insulin resistance.

  5. Lipid nanoparticle interactions and assemblies

    Science.gov (United States)

    Preiss, Matthew Ryan

    Novel liposome-nanoparticle assemblies (LNAs) provide a biologically inspired route for designing multifunctional bionanotheranostics. LNAs combine the benefits of lipids and liposomes to encapsulate, transport, and protect hydrophilic and hydrophobic therapeutics with functional nanoparticles. Functional nanoparticles endow LNAs with additional capabilities, including the ability to target diseases, triggered drug release, controlled therapeutic output, and diagnostic capabilities to produce a drug delivery system that can effectively and efficiently deliver therapeutics while reducing side effects. Not only could LNAs make existing drugs better, they could also provide an avenue to allow once promising non-approved drugs (rejected due to harmful side effects, inadequate pharmacokinetics, and poor efficacy) to be safely used through targeted and controlled delivery directly to the diseased site. LNAs have the potential to be stimuli responsive, delivering drugs on command by external (ultrasound, RF heating, etc.) or internal (pH, blood sugar, heart rate, etc.) stimuli. Individually, lipids and nanoparticles have been clinically approved for therapy, such as Doxil (a liposomal doxorubicin for cancer treatment), and diagnosis, such as Feridex (an iron oxide nanoparticle an MRI contrast enhancement agent for liver tumors). In order to engineer these multifunctional LNAs for theranostic applications, the interactions between nanoparticles and lipids must be better understood. This research sought to explore the formation, design, structures, characteristics, and functions of LNAs. To achieve this goal, different types of LNAs were formed, specifically magnetoliposomes, bilayer decorated LNAs (DLNAs), and lipid-coated magnetic nanoparticles (LMNPs). A fluorescent probe was embedded in the lipid bilayer of magnetoliposomes allowing the local temperature and membrane fluidity to be observed. When subjected to an electromagnetic field that heated the encapsulated iron

  6. 甘草黄酮对2型糖尿病大鼠血糖、血脂等生化指标的影响%Effect of licorice flavonoids on blood glucose, blood lipid and other biochemical indicators in type 2 diabetic rats

    Institute of Scientific and Technical Information of China (English)

    赵海燕; 王勇; 吴力武; 马永平

    2012-01-01

    Objective To study the effect of licorice flavonoids (LF) on fasting blood levels of glucose (FBG), lipids, insulin, adipokines and other biochemical indicators. Methods The type 2 diabetic rat model was replicated by feeding high-fat and high-sugar diet combined with low-dose streptozotocin (STZ) injection. The diabetic rats were divided into model group (DM), high-dose LF treatment group (HLF) and low-dose LF treatment group (LLF). Other 20 normal rats were divided into control group (Con) and control treatment group (CLF). Rats in different groups were administered LF (lOOmg or 300mg ? Kg"1 ? D"1) or solvent (propylene glycol) respectively. Five weeks later, the levels of fasting levels of glucose (FBG), triglycerides (TG), total cholesterol (TO, free fatty acids (FFA), high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol (LDL-C) , total antioxidation capability (T-AOC), insulin (Ins), tumor necrosis factor alpha (TNF-a) and leptin were examined. Insulin sensitivity index (ISI), liver index, kidney index and the relative weight of abdominal adipose tissue were calculated. Results The high-dose LF (300mg ? Kg"1 ? D~' ) treatment significantly decreased the levels of FBG, TG, TC, LDL-C, FFA, T-AOC, TNF-a, leptin, liver index and kidney index, and increased the levels of T-AOC, ISI and the relative weight of abdominal adipose tissue. Compared with high-dose treatment, low dose of LF was less effective. Conclusion LF significantly decreases the blood glucose level, improves the lipid metabolic disorders and ameliorates insulin resistance in type 2 diabetic rats.%目的 研究甘草黄酮(LF)对T2DM大鼠血糖、血脂等生化指标的影响.方法 将实验大鼠分为模型组(DM)、高剂量给药组(HLF)、低剂量给药组(LLF)、正常对照组(Con)和正常给药组(CLF).分别以LF或溶剂灌胃,5周后检测实验大鼠的空腹血糖、血脂等生化指标及肝体比、肾体比和脂体比.结果 高剂量LF(300 mg·kg-1

  7. 长沙地区健康体检人员血脂知识、态度、行为状况及影响因素%Knowledge, attitude and behavior on blood lipid among people participated in health examination in Changsha and the influential factors

    Institute of Scientific and Technical Information of China (English)

    韩辉武; 赵丽群; 虞仁和; 陈能凤; 刘云; 贺连香; 肖莹; 周诗

    2014-01-01

    目的:了解长沙地区健康体检人员血脂知识、态度、行为的现状及主要影响因素,为临床血脂异常的预防与控制提供依据。方法:随机抽取400例来自长沙地区湘雅医院参加健康体检的普通成人,进行血脂知识、态度、行为问卷调查,同时进行血脂相关的体格检查,根据性别、年龄、文化程度、婚姻、家庭月收入分组,并分析影响血脂知识、态度、行为的相关因素。结果:健康体检人员血脂知识得分为(18.33±8.67)分(总分37分),血脂态度得分为(6.63±2.45)分(总分9分),血脂行为得分为(8.32±2.65)分(总分16分)。其中女性知识、行为得分均高于男性(均P<0.05);40~49岁年龄组知识、态度、行为得分均低于其他各年龄组(均P<0.05);初中文化组知识、态度和行为得分均低于其他文化程度组(均P<0.05);月收入2000元以下家庭收入组知识、态度、行为得分均低于其他收入组(均P<0.05)。多元逐步回归分析显示:健康体检人员血脂知识得分主要受文化程度、年龄2个因素影响(均P<0.05),血脂态度得分主要受文化程度、收缩压、血糖及三酰甘油4个因素影响(均P<0.05),血脂行为得分主要受文化程度、三酰甘油、舒张压、血糖及年龄5个因素影响(均P<0.05)。结论:血脂知识、态度、行为主要受文化程度、性别、年龄因素影响,因此医护人员可通过健康教育、提高普通人群相关知识水平来预防血脂异常,且应将40~49岁这一年龄段的男性作为重点干预对象。血脂知识、态度和行为还与个体的血压、血糖和三酰甘油相关,医护人员还应通过提高普通人群血脂、血压及血糖知识水平来改变其相关态度和不良生活行为习惯。此外,应积极控制其血压、血糖来提高普通人群的整体健康状况。%Objective: To evaluate the knowledge, attitude and behavior on blood lipid

  8. Blood-lipid levels of 20041 kindergarten children aged 3 to 6 in Tianjin%天津市20041名3~6岁入托儿童血脂水平分析

    Institute of Scientific and Technical Information of China (English)

    乔毅娟; 王华; 刘恩庆; 张晓燕; 潘蕾; 付伯津; 王萍; 田祯

    2012-01-01

    Objective To understand the level of blood-lipid and prevalence of dyslipidcmia of children aged 3 to 6 in Tianjin,so as to provide evidence for large-scale blood screening strategy and to develop intervention of dyslipidemia and cardiovascular in children.Methods 20 041 children aged 3 to 6 from 48 kindergartens were involved in this study,in Tianjin.Peripheral blood was collected from right leech-finger of these children,after fatless breakfast.Total cholesterol (TC) and triglyceride (TG) of plasma were tested using Toshiba 120 Automatic Biochemical Analyzer.Results The average levels of TC and TG were (4.17 ±0.69)mmol/L and (0.86±0.44) mmol/L in these children.11.4% of the children had either TC or TG dyslipidemia,with 7.1% had only TC dyslipidemia,4.9% had only TG dyslipidemia,and 0.6% of them had both TC and TG dyslipidemia.The prevalence of TC dyslipidemia was significantly higher among girls than boys.The prevalence rates of TC dyslipidemia and TG dyslipidcmia were different among age groups,but with no significant changes among age groups.The prevalence of TG dyslipidemia was significantly different,with obese children higher than those with normal or overweight children.Different residential areas seemed to be related to the difference on the prevalence of dyslipidemia.Prevalence of TC dyslipidemia was higher in urban than in rural areas.Prevalence of TG dyslipidemia was higher in rural than urban areas.Conclusion The prevalence of dyslipidemia for children aged 3 to 6 was high in Tianjin,and showed differences among genders,age groups and residential regions.Screening and intervention programs on dyslipidemia should be undertaken routinely in children,in order to prevent adult atherosclerosis and coronary heart disease.%目的 了解天津市3~6岁儿童血脂水平和血脂异常检出率.方法 采集天津市48所幼儿园20041名3~6岁儿童末梢血,采用东芝120全自动生化分析仪检测血浆总胆固醇(TC)

  9. Elastic deformation and failure of lipid bilayer membranes containing cholesterol.

    OpenAIRE

    Needham, D; Nunn, R. S.

    1990-01-01

    Giant bilayer vesicles were reconstituted from several lipids and lipid/cholesterol (CHOL) mixtures: stearolyloleoylphosphatidylcholine (SOPC), bovine sphingomyelin (BSM), diarachidonylphosphatidylcholine (DAPC), SOPC/CHOL, BSM/CHOL, DAPC/CHOL, and extracted red blood cell (RBC) lipids with native cholesterol. Single-walled vesicles were manipulated by micropipette suction and several membrane material properties were determined. The properties measured were the elastic area compressibility m...

  10. APOE polymorphism is associated with lipid profile, but not with arterial stiffness in the general population

    Directory of Open Access Journals (Sweden)

    Mill José G

    2010-11-01

    Full Text Available Abstract Background Cardiovascular diseases (CVD are the main cause of death and disability in developed countries. In most cases, the progress of CVD is influenced by environmental factors and multifactorial inheritance. The purpose of this study was to investigate the association between APOE genotypes, cardiovascular risk factors, and a non-invasive measure of arterial stiffness in the Brazilian population. Methods A total of 1493 urban Brazilian individuals were randomly selected from the general population of the Vitoria City Metropolitan area. Genetic analysis of the APOE polymorphism was conducted by PCR-RFLP and pulse wave velocity analyzed with a noninvasive automatic device. Results Age, gender, body mass index, triglycerides, creatinine, uric acid, blood glucose, blood pressure phenotypes were no different between ε2, ε3 and ε4 alleles. The ε4 allele was associated with higher total-cholesterol (p Conclusion The ε4 allele of the APOE gene is associated with a worse lipid profile in the Brazilian urban population. In our relatively young sample, the observed effect of APOE genotype on lipid levels was not translated into significant effects in arterial wall stiffness.

  11. LIPID PEROXIDATION AND LIPID PROFILE IN PATIENTS WITH SENILE CATARACT

    Directory of Open Access Journals (Sweden)

    Satya Devi

    2015-11-01

    Full Text Available : Lipid peroxidation products and biochemical parameters like fasting blood sugar and lipid profile were estimated in serum of 40 senile cataract cases (45-60 years and 40 persons of age and sex matched healthy controls. Plasma Thiobarbituric acid reactive substances (TBARS levels [as Malondialdehyde (MDA] were significantly higher in patients with senile cataract (573±64.4nmol/dl, P<0.001 as compared to healthy controls (378±31.31 nmol/dl. Serum cholesterol, Triglycerides, LDLC, VLDLC levels were increased as compared to controls. But HDLC levels were decreased in senile cataract patients as compared to controls. No significant change was observed in FBS values. The present study shows that the oxidative stress may play an important role in the senile cataract.

  12. Elevated blood lipids are associated with the severity of coronary stenosis in patients with coronary heart disease%冠心病患者血脂异常与冠状动脉病变的相关性分析

    Institute of Scientific and Technical Information of China (English)

    张玉美; 韩雅玲; 荆全民; 王效增; 李毅; 张磊; 霍勇; 张岩

    2012-01-01

    score with > 50% stenosis in the angiography. Preoperative triglyceride (TG) , total cholesterol (TC) , high density lipoprotein cholesterol ( HDL-C) , low density lipoprotein cholesterol ( LDL-C) levels were measured. Comparative analysis of the coronary lesions and the extent of coronary stenosis with lipid levels was made. Results By analysis of 6419 cases of coronary heart disease, patients with lower HDL-C levels and higher TC, LDL-C levels were found to have more severe coronary lesions and the levels of TC and LDL-C were positively related with the severity of coronary artery stenosis and the Gensini score. Multiple liner regression analysis showed that Gensini score was independently negatively correlated with HDL-C (β= -0. 075,OR: -5. 580, 95%CI: -7.541 - - 3. 618,P < 0. 001 ) but independently positively correlated with LDL-C (/3=0.067, OR:2. 712,95%CI:0. 943 -4.481,P =0.003). TG (β = 0. OO2,OK:0. 067,95% CI: -0. 689 -0. 822,P =0.863) and TC (β = 0. 034, OR: 1. 080,95% CI: -0.392 -2.553,P=0. 150) showed no correlation with Gensini score. Conclusions TG, LDL-C, TC as an independent risk factor for coronary heart disease.Gensini score was independently negatively correlated with HDL-C, but was positively related with LDL-C. TG and TC were not correlated with Gensini score. Control of blood lipids to prevent coronary heart disease showed positive significance.

  13. Transfusion reaction in a case with the rare Bombay blood group

    OpenAIRE

    Hayedeh Javadzadeh Shahshahani; Mohamad Reza Vahidfar; Seyed Ali Khodaie

    2013-01-01

    Bombay phenotype is extremely rare in Caucasian with an incidence of 1 in 250,000. When individuals with the Bombay phenotype need blood transfusion, they can receive only autologous blood or blood from another Bombay blood group. Transfusing blood group O red cells to them can cause a fatal hemolytic transfusion reaction. In this study, we report a case with the rare Bombay blood group that was misdiagnosed as the O blood group and developed a hemolytic transfusion reaction. This highlights ...

  14. Blood pressure

    Science.gov (United States)

    ... the walls of the arteries is called blood pressure. Blood pressure is measured both as the heart contracts, which ... as it relaxes, which is called diastole. Normal blood pressure is considered to be a systolic blood pressure ...

  15. Blood transfusions

    Science.gov (United States)

    ... this page: //medlineplus.gov/ency/patientinstructions/000431.htm Blood transfusions To use the sharing features on this ... several sources of blood which are described below. Blood From the Public (Volunteer Blood Donation) The most ...

  16. Blood Basics

    Science.gov (United States)

    ... Patient Group Links Advocacy Toolkit Home For Patients Blood Basics Blood is a specialized body fluid. It ... about 9 pints. Jump To: The Components of Blood and Their Importance Many people have undergone blood ...

  17. Blood Thinners

    Science.gov (United States)

    If you have some kinds of heart or blood vessel disease, or if you have poor blood flow to your brain, your doctor may recommend that you take a blood thinner. Blood thinners reduce the risk of heart ...

  18. Blood culture

    Science.gov (United States)

    Culture - blood ... A blood sample is needed . The site where blood will be drawn is first cleaned with an antiseptic such ... organism from the skin getting into (contaminating) the blood sample and causing a false-positive result (see ...

  19. Perbaikan Parameter Lipid Darah Mencit Hiperkolesterolemia dengan Suplemen Pangan Bekatul

    OpenAIRE

    [None] Herawati; Wasmen Manalu; Agik Suprayogi; Dewi Apri Astuti

    2013-01-01

    Dietary fiber is widely used as a functional food and recommended to reduce blood lipid level to prevent hypercholesterolemia. An experiment was conducted on the effects of rice bran that has high dietary fiber content on blood lipid parameters of hypercholesterolemic male mice, which was conducted at the Biology Education Department animal cages, Indonesia University of Education and the Veterinary Medicine Faculty Laboratory, Bogor Agricultural Institute from August 2011 to March 2012. The ...

  20. A comparison of the effect of free access to reduced fat products or their full fat equivalents on food intake, body weight, blood lipids and fat-soluble antioxidants levels and haemostasis variables

    NARCIS (Netherlands)

    Weststrate, J.A.; Hof, K.H. van het; Berg, H. van den; Velthuis-te-Wierik, E.J.M. te; Graaf, C. de; Zimmermanns, N.J.H.; Westerterp, K.R.; Westerterp-Plantenga, M.S.; Verboeket-Venne, W.P.H.G. van de

    1998-01-01

    Objectives: To compare the effects of free access to reduced fat products or their full fat equivalents on fat and energy intake, body weight, plasma lipids and fat-soluble antioxidants concentrations and haemostasis variables. Design: A multicentre open randomised controlled trial in which interven

  1. Improved characterization of EV preparations based on protein to lipid ratio and lipid properties.

    Directory of Open Access Journals (Sweden)

    Xabier Osteikoetxea

    Full Text Available In recent years the study of extracellular vesicles has gathered much scientific and clinical interest. As the field is expanding, it is becoming clear that better methods for characterization and quantification of extracellular vesicles as well as better standards to compare studies are warranted. The goal of the present work was to find improved parameters to characterize extracellular vesicle preparations. Here we introduce a simple 96 well plate-based total lipid assay for determination of lipid content and protein to lipid ratios of extracellular vesicle preparations from various myeloid and lymphoid cell lines as well as blood plasma. These preparations included apoptotic bodies, microvesicles/microparticles, and exosomes isolated by size-based fractionation. We also investigated lipid bilayer order of extracellular vesicle subpopulations using Di-4-ANEPPDHQ lipid probe, and lipid composition using affinity reagents to clustered cholesterol (monoclonal anti-cholesterol antibody and ganglioside GM1 (cholera toxin subunit B. We have consistently found different protein to lipid ratios characteristic for the investigated extracellular vesicle subpopulations which were substantially altered in the case of vesicular damage or protein contamination. Spectral ratiometric imaging and flow cytometric analysis also revealed marked differences between the various vesicle populations in their lipid order and their clustered membrane cholesterol and GM1 content. Our study introduces for the first time a simple and readily available lipid assay to complement the widely used protein assays in order to better characterize extracellular vesicle preparations. Besides differentiating extracellular vesicle subpopulations, the novel parameters introduced in this work (protein to lipid ratio, lipid bilayer order, and lipid composition, may prove useful for quality control of extracellular vesicle related basic and clinical studies.

  2. A rare case of haemolytic disease of newborn with Bombay phenotype mother

    Directory of Open Access Journals (Sweden)

    Shamee Shastry

    2013-01-01

    Full Text Available We are reporting a rare case of severe hemolytic disease of newborn (HDN with Bombay phenotype mother. A retrospective study of a case with severe haemolytic disease of newborn with Bombay phenotype mother was done. Blood grouping, antibody screening, and lectin study was done on the blood sample of the baby and mother to confirm the diagnosis. Hematological and biochemical parameters were obtained from the hospital laboratory information system for the analysis. Blood group of the baby was A positive, direct antiglobulin test was negative. Blood group of the mother was confirmed to be Bombay phenotype, Hematological parameters showed all the signs of ongoing hemolysis and the bilirubin level was in the zone of exchange transfusion. Due to the unavailability of this rare phenotype blood unit, baby was managed conservatively. Anticipating the fetal anemia and HDN with mothers having Bombay phenotype and prior notification to the transfusion services will be of great help in optimizing the neonatal care and outcome.

  3. 限制食用油摄入量对糖尿病患者血糖和血脂的影响%A Study on the Effect of Limiting the Intake of Edible Oil on Blood Glucose and Lipids of Patients with Diabetes

    Institute of Scientific and Technical Information of China (English)

    任海清; 胡丽燕; 张片红; 缪琴; 何芳; 金星

    2013-01-01

    目的 研究限制每天食用油的摄入量对糖尿病患者血糖和血脂的影响.方法 对63例糖尿病患者采用自身对照方法,在接受食用油限量前后检测受试者血糖和血脂等指标.结果 限制每天食用油的摄入量后,受试者血糖水平从(7.88±2.32) mmol/L降至(7.02±2.00) mmol/L (P<0.05),血中总胆固醇(TC)、高密度脂蛋白(HDL)和低密度脂蛋白(LDL)水平分别从(5.13±0.93)、(1.33±0.38)和(2.55±0.58) mmol/L降至(4.69±0.89)、(1.20±0.35)和(2.29±0.53)mmol/L (P<0.01),但甘油三酯没有降低.结论 限制食用油的摄入量能有效降低血糖和血脂的水平.%Objective To explore the effect of limiting the daily intake of edible oil on blood glucose and lipid of patients with diabetes.Methods A self-control study was conducted on 63 patients with diabetes.The blood glucose and lipids were detected before and after limiting the edible oil.Results After limiting the daily intake of edible oil,the blood glucose level fell from 7.88 ± 2.32 mmol/L to7.02 ±2.00 mmol/L (P < 0.05).The serum total cholesterol (TC),high density lipoprotein (HDL) and low density lipoprotein (LDL) levels fell from 5.13 ±0.93,1.33 ±0.38 and 2.55 ±0.58 mmol/L to 4.69 ±0.89,1.20 ±0.35 and 2.29 ±0.53 mmol/L(P <0.01),respectively.No significant reduction of triglycerides was found.Conclusion Limiting the daily intake of edible oil can effectively reduce the level of blood glucose and lipids.

  4. Anti-inflammatory activity of cationic lipids.

    Science.gov (United States)

    Filion, M C; Phillips, N C

    1997-10-01

    1. The effect of liposome phospholipid composition has been assumed to be relatively unimportant because of the presumed inert nature of phospholipids. 2. We have previously shown that cationic liposome formulations used for gene therapy inhibit, through their cationic component, the synthesis by activated macrophages of the pro-inflammatory mediators nitric oxide (NO) and tumour necrosis factor-alpha (TNF-alpha). 3. In this study, we have evaluated the ability of different cationic lipids to reduce footpad inflammation induced by carrageenan and by sheep red blood cell challenge. 4. Parenteral (i.p. or s.c) or local injection of the positively charged lipids dimethyldioctadecylammomium bromide (DDAB), dioleyoltrimethylammonium propane (DOTAP), dimyristoyltrimethylammonium propane (DMTAP) or dimethylaminoethanecarbamoyl cholesterol (DC-Chol) significantly reduced the inflammation observed in both models in a dose-dependent manner (maximum inhibition: 70-95%). 5. Cationic lipids associated with dioleyol- or dipalmitoyl-phosphatidylethanolamine retained their anti-inflammatory activity while cationic lipids associated with dipalmitoylphosphatidylcholine (DPPC) or dimyristoylphosphatidylglycerol (DMPG) showed no anti-inflammatory activity, indicating that the release of cationic lipids into the macrophage cytoplasm is a necessary step for anti-inflammatory activity. The anti-inflammatory activity of cationic lipids was abrogated by the addition of dipalmitoylphosphatidylethanolamine-poly(ethylene)glycol-2000 (DPPE-PEG2000) which blocks the interaction of cationic lipids with macrophages. 6. Because of the significant role of protein kinase C (PKC) in the inflammatory process we have determined whether the cationic lipids used in this study inhibit PKC activity. The cationic lipids significantly inhibited the activity of PKC but not the activity of a non-related protein kinase, PKA. The synthesis of interleukin-6 (IL-6), which is not dependent on PKC activity for its

  5. Prenatal hyperandrogenism induces alterations that affect liver lipid metabolism.

    Science.gov (United States)

    Abruzzese, Giselle Adriana; Heber, Maria Florencia; Ferreira, Silvana Rocio; Velez, Leandro Martin; Reynoso, Roxana; Pignataro, Omar Pedro; Motta, Alicia Beatriz

    2016-07-01

    Prenatal hyperandrogenism is hypothesized as one of the main factors contributing to the development of polycystic ovary syndrome (PCOS). PCOS patients have high risk of developing fatty liver and steatosis. This study aimed to evaluate the role of prenatal hyperandrogenism in liver lipid metabolism and fatty liver development. Pregnant rats were hyperandrogenized with testosterone. At pubertal age, the prenatally hyperandrogenized (PH) female offspring displayed both ovulatory (PHov) and anovulatory (PHanov) phenotypes that mimic human PCOS features. We evaluated hepatic transferases, liver lipid content, the balance between lipogenesis and fatty acid oxidation pathway, oxidant/antioxidant balance and proinflammatory status. We also evaluated the general metabolic status through growth rate curve, basal glucose and insulin levels, glucose tolerance test, HOMA-IR index and serum lipid profile. Although neither PH group showed signs of liver lipid content, the lipogenesis and fatty oxidation pathways were altered. The PH groups also showed impaired oxidant/antioxidant balance, a decrease in the proinflammatory pathway (measured by prostaglandin E2 and cyclooxygenase-2 levels), decreased glucose tolerance, imbalance of circulating lipids and increased risk of metabolic syndrome. We conclude that prenatal hyperandrogenism generates both PHov and PHanov phenotypes with signs of liver alterations, imbalance in lipid metabolism and increased risk of developing metabolic syndrome. The anovulatory phenotype showed more alterations in liver lipogenesis and a more impaired balance of insulin and glucose metabolism, being more susceptible to the development of steatosis. PMID:27179108

  6. Vincristine Lipid Complex Injection

    Science.gov (United States)

    Vincristine lipid complex is used to treat a certain type of acute lymphoblastic leukemia (ALL; a type ... at least two different treatments with other medications. Vincristine lipid complex is in a class of medications ...

  7. Irinotecan Lipid Complex Injection

    Science.gov (United States)

    Irinotecan lipid complex is used in co