WorldWideScience

Sample records for blood glucose serum

  1. Carbon dot based non enzymatic approach for the detection and estimation of glucose in blood serum

    Science.gov (United States)

    Shanti Krishna, A.; Nair, Priya A.; Radhakumary, C.; Sreenivasan, K.

    2016-05-01

    In this study we generated a simple, reliable and selective approach based on carbon dots (CDs) and 4-cyanophenylboronic acid (CPBA) for blood glucose sensing. The methodology relies on the quenching of the emission of CDs by CPBA followed by its recovery by glucose. The system consisting of CDs and CPBA was characterised by Fourier transform infra red spectrum, transmissions electron microscopic, dynamic light scattering instrument, UV–visible and fluorescence techniques. The response of the probe, CD-BA in presence of different concentrations of glucose was assessed. Linear range was obtained for glucose concentrations ranging from 1 to 30 mM. Interferences by other saccharides and various biomolecules coexisting in blood serum were negligible. The chemo sensor thus developed has been successfully used for the estimation of glucose in human blood serum. The system being sensitive, efficient and easy to perform is a promising platform for blood glucose sensing.

  2. Clinical significance of determination of serum leptin, insulin levels and blood sugar in pregnant women with glucose metabolism disturbances

    International Nuclear Information System (INIS)

    Objective: To investigate the changes of serum leptin, insulin levels and blood sugar contents in pregnant women with gestational glucose metabolism disturbances. Methods: Fasting and 3h after oral 50g glucose serum levels of leptin were measured with RIA in 36 pregnant women with glucose metabolism disturbances (gestational diabetes mellitus or gestational impaired glucose tolerance) and 34 controls. Also, fasting serum insulin levels (with CLIA) and blood sugar contents 1h after oral 50 glucose (with glucose oxidase method) were determined in all these subjects. Results: 1. Serum levels of leptin in pregnant women with glucose metabolism disturbances were 14.9 ± 4.3 μg/L (vs controls 9.8 ± 1.7 μg/L, P<0.01). 2. The serum levels of insulin and 1 h post - 50g glucose blood sugar contents in pregnant women with glucose metabolism disturbances were 12.9±4.3mU/L and 11.0±1.4mmol/L respectively, which were both significantly positively correlated with the serum leptin levels (r=0.835, r=0.758 respectively) (vs levels in controls: 8.45±3.0mU/L and 7.84±1.3mmol/L). Conclusion: Elevation of fasting serum levels of leptin was demonstrated in pregnant women with glucose metabolism disturbances and the level of leptin was positively correlated with that of insulin and blood sugar. (authors)

  3. Investigation the effect of Commiphora mukul on blood glucose and Serum lipid profile in diabetic rats

    Directory of Open Access Journals (Sweden)

    leila Shirazi

    2015-01-01

    Full Text Available Background & aim: The decrease of serum glucose level and lipids in diabetic patients is clinically significant. The purpose of this study was to determine the effect of Commiphora mukul extract on blood sugar and lipid profile in diabetic rats. Methods: In the present experimental study, thirty-two male Wistar rats were randomly divided into four groups: control, control treated with the extract, diabetic and diabetic treated with the extract. Diabetes was induced by intraperitoneal injection of Streptozotocin. In an eight week period, the control group of normal saline and the control group and diabetic recipient extract of CM oleo gum blue resin was given by gavage. Treatment resumed eight weeks with onset of hyperglycemia. The control and diabetic control groups received normal saline orally. Extract treated control and extract treated diabetic groups received extract of Commiphora mukul gum (300 mg/kg P.O. daily by gavage. At the end of this period, blood samples were collected from each rat and biochemical tests for investigation of glucose level and lipid profile was performed. One- way analysis of variance (ANOVA statistical test and Post-hoc test Tukey’s were used for data analysis Results: The study indicated that diabetes increases the serum levels of glucose, cholesterol, triglycerides, LDL and HDL. Administration of Commiphora mukul gum extract in diabetic groups significantly decreased the serum level of glucose, cholesterol(p<0.01 and triglyceride and LDL(p<0.001 and increased HDL(p<0.01. Conclusion: Commiphora mukul gum extract may well improve undesirable effects of diabetes on serum level of glucose, cholesterol, triglyceride, LDL and HDL.

  4. GUAR GUM EFFECTS ON BLOOD SERUM LIPIDS AND GLUCOSE CONCENTRATIONS OF WISTAR DIABETIC RATS1

    Directory of Open Access Journals (Sweden)

    DARIO FRIAS Andrea

    1998-01-01

    Full Text Available The effects of guar gum derived from the endosperm of Cyamopsis tetragonoloba (75% soluble fiber, 7.6% insoluble fiber, 2.16% crude protein, 0.78% total lipids, 0.54% ash and 9.55% moisture on diabetic rats were studied concerning food intake, body weight gain, blood serum cholesterol, triacylglycerols, glucose, LDL-, and HDL-cholesterol concentrations. The effect of gum on indexes of protein absorption and utilization was also investigated. Diets containing 0%, 10% and 20% (w/w guar gum were fed to diabetic rats for 28 days. In spite of the fact that diabetes elevated blood lipids in all animals, guar gum diet significantly decreased (p <0.05 serum concentrations of cholesterol and triacylglycerols. Furthermore, a concomitant increase in HDL-cholesterol with a substancial elevation of the HDL/LDL cholesterol ratio was found. The most significant result in this assay was the drastic reduction of blood glucose in diabetic rats treated with guar gum diet. The gum promoted a general improvement in the condition of the diabetic rats, in body weight gain and indexes of protein absorption and utilization. The results of this research suggest that guar gum, at concentrations equal to or higher than 10%, should be effective in the treatment of hypercholesterolemia and diabetes, in humans.

  5. Study of the Effect of Garlic on Serum Lipids and Blood Glucose Levels in Type 2 Diabetic Patients

    Directory of Open Access Journals (Sweden)

    M Afkhami - Ardekani

    2005-04-01

    Full Text Available Introduction: Hyperlipidemia and diabetes are common risk factors for ischemic heart disease, which is the main cause of mortality in diabetic patients. Strict control of blood glucose and other risk factors in diabetics has led to prevention of complications. Garlic has received particular attention for control of blood glucose and decrease in blood lipid levels. At present, several studies have been carried out in order to prove advantages of garlic. Methods: In this study, effects of garsin (a derivative of garlic present in our country on serum lipids and blood glucose levels in diabetes mellitus type 2 patients was observed. Forty-five type 2 diabetics who had hyperlipidemia were selected. These patients were kept on treatment with 3 tablets of Garsin / day for 4 weeks. Serum lipids and blood glucose levels were measured prior to and at the end of treatment. Results: Relationship between sex and response to treatment in this study was meaningful, such that Gsarsin led to decrease in LDL-C and increase in HDL in females. Conclusion: Therefore, Garsin can be used as an adjunct to treatment in diabetes type 2 patients with hyperlipidemia.

  6. Low Blood Glucose (Hypoglycemia)

    Science.gov (United States)

    ... Other Dental Problems Diabetic Eye Disease Low Blood Glucose (Hypoglycemia) What is hypoglycemia? Hypoglycemia, also called low ... actions can also help prevent hypoglycemia: Check blood glucose levels Knowing your blood glucose level can help ...

  7. Determination of total creatine kinase activity in blood serum using an amperometric biosensor based on glucose oxidase and hexokinase.

    Science.gov (United States)

    Kucherenko, I S; Soldatkin, O O; Lagarde, F; Jaffrezic-Renault, N; Dzyadevych, S V; Soldatkin, A P

    2015-11-01

    Creatine kinase (CK: adenosine-5-triphosphate-creatine phosphotransferase) is an important enzyme of muscle cells; the presence of a large amount of the enzyme in blood serum is a biomarker of muscular injuries, such as acute myocardial infarction. This work describes a bi-enzyme (glucose oxidase and hexokinase based) biosensor for rapid and convenient determination of CK activity by measuring the rate of ATP production by this enzyme. Simultaneously the biosensor determines glucose concentration in the sample. Platinum disk electrodes were used as amperometric transducers. Glucose oxidase and hexokinase were co-immobilized via cross-linking with BSA by glutaraldehyde and served as a biorecognition element of the biosensor. The biosensor work at different concentrations of CK substrates (ADP and creatine phosphate) was investigated; optimal concentration of ADP was 1mM, and creatine phosphate - 10 mM. The reproducibility of the biosensor responses to glucose, ATP and CK during a day was tested (relative standard deviation of 15 responses to glucose was 2%, to ATP - 6%, to CK - 7-18% depending on concentration of the CK). Total time of CK analysis was 10 min. The measurements of creatine kinase in blood serum samples were carried out (at 20-fold sample dilution). Twentyfold dilution of serum samples was chosen as optimal for CK determination. The biosensor could distinguish healthy and ill people and evaluate the level of CK increase. Thus, the biosensor can be used as a test-system for CK analysis in blood serum or serve as a component of multibiosensors for determination of important blood substances. Determination of activity of other kinases by the developed biosensor is also possible for research purposes. PMID:26452867

  8. Determination of total creatine kinase activity in blood serum using an amperometric biosensor based on glucose oxidase and hexokinase

    OpenAIRE

    Kucherenko, Ivan S; Soldatkin, Oleksandr O; Lagarde, Florence; Jaffrezic-Renault, Nicole; Dzyadevych, S.V.; Soldatkin, A.P.

    2015-01-01

    International audience Creatine kinase (CK: adenosine-5-triphosphate-creatine phosphotransferase) is an important enzyme of muscle cells; the presence of a large amount of the enzyme in blood serum is a biomarker of muscular injuries, such as acute myocardial infarction. This work describes a bi-enzyme (glucose oxidase and hexokinase based) biosensor for rapid and convenient determination of CK activity by measuring the rate of ATP production by this enzyme. Simultaneously the biosensor de...

  9. High dose flaxseed oil supplementation may affect fasting blood serum glucose management in human type 2 diabetics.

    Science.gov (United States)

    Barre, Douglas E; Mizier-Barre, Kazimiera A; Griscti, Odette; Hafez, Kevin

    2008-01-01

    Type 2 diabetes is characterized partially by elevated fasting blood serum glucose and insulin concentrations and the percentage of hemoglobin as HbA1c. It was hypothesized that each of blood glucose and its co-factors insulin and HbA1c and would show a more favorable profile as the result of flaxseed oil supplementation. Patients were recruited at random from a population pool responding to a recruitment advertisement in the local newspaper and 2 area physicians. Completing the trial were 10 flaxseed oil males, 8 flaxseed oil females, 8 safflower (placebo) oil males and 6 safflower oil females. Patients visited on two pre-treatment occasions each three months apart (visits 1 and 2). At visit 2 subjects were randomly assigned in double blind fashion and in equal gender numbers to take flaxseed oil or safflower oil for three further months until visit 3. Oil consumption in both groups was approximately 10 g/d. ALA intake in the intervention group was approximately 5.5 g/d. Power was 0.80 to see a difference of 1 mmol of glucose /L using 12 subjects per group with a p impact on fasting blood serum glucose, insulin or HbA1c levels. It is concluded that high doses of flaxseed oil have no effect on glycemic control in type 2 diabetics. PMID:18391475

  10. Hyperglycemia (High Blood Glucose)

    Medline Plus

    Full Text Available ... Blood Pressure Physical Activity High Blood Glucose My Health Advisor Tools To Know Your Risk Alert Day ... DKA (Ketoacidosis) & Ketones Kidney Disease (Nephropathy) Gastroparesis Mental Health Step On Up Treatment & Care Blood Glucose Testing ...

  11. Hyperglycemia (High Blood Glucose)

    Medline Plus

    Full Text Available ... symptoms include the following: High blood glucose High levels of sugar in the urine Frequent urination Increased ... you should check and what your blood glucose levels should be. Checking your blood and then treating ...

  12. Blood Test: Glucose

    Science.gov (United States)

    ... Things to Know About Zika & Pregnancy Blood Test: Glucose KidsHealth > For Parents > Blood Test: Glucose Print A A A Text Size What's in ... de sangre: glucosa What It Is A blood glucose test measures the amount of glucose (the main ...

  13. Clinical significance of serum carbohydrate antigen 19-9 and its relationship with blood glucose in patients with type 2 diabetes

    Institute of Scientific and Technical Information of China (English)

    武秀玲

    2013-01-01

    Objective To study the relationship between serum carbohydrate antigen 19-9 (CA19-9) and blood glucose level in patients with type 2 diabetes (T2DM) .Methods Totally 784 T2DM patients and 197 healthy controls were enrolled in this study.Age,duration,body mass index (BMI) ,systolic blood pressure (SBP) ,diastolic blood pressure (DBP) ,levels of fasting blood glucose (FBG) ,postprandial blood glucose (PBG) ,hemoglobin A1c (HbA1c) ,total cholesterol (TC) ,triglyceride (TG) ,

  14. Hyperglycemia (High Blood Glucose)

    Medline Plus

    Full Text Available ... how often you should check and what your blood glucose levels should be. Checking your blood and then treating ... I Treat Hyperglycemia? You can often lower your blood glucose level by exercising. However, if your blood glucose is ...

  15. Related research of blood glucose, blood lipid and serum cystatin C and lower-extremity arterial disease in type 2 diabetic mellitus

    Institute of Scientific and Technical Information of China (English)

    Juan Zeng; Jie Ou; Yun-Mei He; Chun-Yu Cai

    2016-01-01

    Objective:To explore the relationship between levels of blood glucose, blood lipid and serum cystatin C and lower-extremity arterial disease in type 2 diabetic mellitus so as to provide the basis for the prevention and treatment of the disease.Methods:A total of 240 cases of patients with type 2 diabetic mellitus receiving physical examinations in our hospital from March 2014 to March 2015 were selected and divided into three groups. The control group was consisted of type 2 diabetes patients without lower-extremity arterial disease, the observation group 1 included type 2 diabetes patients with mild or moderate lower-extremity arterial disease, and the observation group 2 was formed by type 2 diabetes patients with severe lower-extremity arterial disease. Each group contained 80 cases. Then, the heights, weights, waist and hip circumferences of patients in the three groups were determined and the body mass index (BMI) and waist to hip ratio (WHR) were calculated; the fasting blood glucose (FBG), fasting insulin (FINS), insulin resistance index (HOMA-IR), 2 h postprandial blood glucose (2 h PG) and glycosylated hemoglobin (HBA1c) in two groups were detected; and the levels of serum total cholesterol(TC), triglyceride (TG), low density lipoprotein cholesterol (LDL-C), high density lipoprotein cholesterol (HDL-C), apolipoprotein A1 (apoA1), apolipoprotein B (apoB) and cystatin C (CysC) in two groups were also tested.Results:Compared with the control group, the WHR, FBG, HOMA-IR, 2 h PG, HBA1c, LDL-C, apoB and CysC all increased significantly, and the differences were statistically significant. Besides, there were statistically significances existing between the observation groups 1 and 2. What’s more, the serum levels of TC, HDL-C, TG and apoA1 in the observation groups were not significantly different from those of the control group.Conclusions: There is a close relationship between blood glucose, blood lipid, serum cystatin C level and lower-extremity arterial

  16. Hyperglycemia (High Blood Glucose)

    Medline Plus

    Full Text Available ... and eAG Hypoglycemia (Low blood glucose) Hyperglycemia (High blood glucose) Dawn Phenomenon Checking for Ketones Tight Diabetes Control donate en -- Diabetes Must Be Stopped - 2016-06-donation- ...

  17. Hyperglycemia (High Blood Glucose)

    Medline Plus

    Full Text Available ... Health Step On Up Treatment & Care Blood Glucose Testing Medication Doctors, Nurses & More Oral Health & Hygiene Women ... Living With Diabetes > Treatment and Care > Blood Glucose Testing Share: Print Page Text Size: A A A ...

  18. Hyperglycemia (High Blood Glucose)

    Medline Plus

    Full Text Available ... by Mail Close www.diabetes.org > Living With Diabetes > Treatment and Care > Blood Glucose Testing Share: Print Page Text Size: ... and-how-tos, In this section Living With Diabetes Treatment and Care Blood Glucose Testing Checking Your Blood Glucose A1C ...

  19. Hyperglycemia (High Blood Glucose)

    Science.gov (United States)

    ... by Mail Close www.diabetes.org > Living With Diabetes > Treatment and Care > Blood Glucose Testing Share: Print Page Text Size: ... and-how-tos, In this section Living With Diabetes Treatment and Care Blood Glucose Testing Checking Your Blood Glucose A1C ...

  20. Oligosaccharide and Creatine Supplementation on Glucose and Urea Nitrogen in Blood and Serum Creatine Kinase in Basketball Athletes

    Institute of Scientific and Technical Information of China (English)

    2005-01-01

    The effects of oligosaccharide and creatine (Cr) supplementation on glucose, lactic acid and urea nitrogen levels in blood and activity of serum creatine kinase (CK) were explored. Twenty CUBA male athletes were divided into 4 groups: group A (supplementation of Cr alone), group B (supplementation of oligosaccharide), group C (supplementation of oligosaccharide and Cr) and group D (placebo control group). By using orthogonal L4 table (23), the experiment was performed. There were factors including oligosaccharide (carbohydrate, CHO), Cr and their correlation. Each factor had two levels: supplementation and no-supplementation. The results showed that the supplementation of CHO or Cr alone, combined supplementation of CHO and Cr could significantly reduce the glucose, urea nitrogen levels in blood and serum CK activity after competition in the athletes. Moreover, the effects of combined supplementation of CHO and Cr were more satisfactory. It was concluded that supplementation of CHO and Cr could promote the recovery of physical performance and athletic abilities after athletics in basketball athletes.

  1. The Comparative Evaluation of the Effect of the Foods with Different Glycemic Indices on Blood Glucose and Serum Free Fatty Acids in Cycling, Male Athletes

    Directory of Open Access Journals (Sweden)

    Asadi, J. (PhD

    2014-05-01

    Full Text Available Background and Objective: Carbohydrates are considered as the major source of energy in physical activity. Studies show that consumption of carbohydrate foods before exercise can balance blood glucose and free fatty acids and increase athletes’ performance. In this study , we compared the effect of three kinds of foods with different glycemic indices on blood glucose (BG and serum free fatty acids (FFA in cycling ,male athletes. Material and Methods: In this clinical trial, 21 members of national cycling team randomly allocated to three equal groups of glucose (low glycemic index ، lentil (low glycemic index and potato (high glycemic index. First, Fasting blood samples (5ml were obtained to measure BG and FFA . Then the subjects were asked to eat their foods. After 45 mins of rest, they pedaled with maximal oxygen consumption VO2max for two hours and again their blood samples were taken to compare with the levels of before interventions. Results: Glucose consumption resulted in a significant decrease in FFA level after 2 hours of pedaling (P = 0.01 but no significant change in BG level. Plasma glucose was higher after eating lentil than that of potato (P<0.05, but it was not true for FFA level of both groups. Conclusion: Based on the results, the pre-exercise use of low glycemic index (lentil compared to high glycemic index (potato can better lead to increased blood glucose during exercise. Keywords: Glycemic Index; Blood Glucose; Serum Free Fatty Acids; Cyclists

  2. Hyperglycemia (High Blood Glucose)

    Medline Plus

    Full Text Available ... but it is not as effective as it should be. You ate more than planned or exercised ... glucose often. Ask your doctor how often you should check and what your blood glucose levels should ...

  3. Hyperglycemia (High Blood Glucose)

    Medline Plus

    Full Text Available ... Complications DKA (Ketoacidosis) & Ketones Kidney Disease (Nephropathy) Gastroparesis Mental Health Step On Up Treatment & Care Blood Glucose ... glucose) Dawn Phenomenon Checking for Ketones Tight Diabetes Control donate en -- Your Gift for Research Doubled - 2016- ...

  4. Monitor blood glucose - slideshow

    Science.gov (United States)

    ... medlineplus.gov/ency/presentations/100220.htm Monitoring blood glucose - Series—Monitoring blood glucose: Using a self-test meter To use the ... A.M. Editorial team. Related MedlinePlus Health Topics Blood Sugar A.D.A.M., Inc. is accredited by ...

  5. Hyperglycemia (High Blood Glucose)

    Medline Plus

    Full Text Available ... by Mail Close www.diabetes.org > Living With Diabetes > Treatment and Care > Blood Glucose Testing Share: Print Page ... and-how-tos, In this section Living With Diabetes Treatment and Care Blood Glucose Testing Checking Your Blood ...

  6. Hyperglycemia (High Blood Glucose)

    Medline Plus

    Full Text Available ... Nephropathy) Gastroparesis Mental Health Step On Up Treatment & Care Blood Glucose Testing Medication Doctors, Nurses & More Oral Health & Hygiene Women A1C Insulin Pregnancy ...

  7. Hyperglycemia (High Blood Glucose)

    Medline Plus

    Full Text Available ... Disease (Nephropathy) Gastroparesis Mental Health Step On Up Treatment & Care Blood Glucose Testing Medication Doctors, Nurses & More Oral Health & Hygiene Women A1C Insulin ...

  8. Hyperglycemia (High Blood Glucose)

    Medline Plus

    Full Text Available ... Overweight Smoking High Blood Pressure Physical Activity High Blood Glucose My Health Advisor Tools To Know Your Risk Alert Day Diabetes Basics Home Symptoms Diagnosis America's Diabetes Challenge Type 1 Type 2 Facts About Type 2 Enroll ...

  9. Hyperglycemia (High Blood Glucose)

    Medline Plus

    Full Text Available ... Risk Healthy Eating Overweight Smoking High Blood Pressure Physical Activity High Blood Glucose My Health Advisor Tools To ... Index Low-Calorie Sweeteners Sugar and Desserts Fitness Exercise & Type 1 Diabetes Get Started Safely Get And ...

  10. Study of the Effect of Garlic on Serum Lipids and Blood Glucose Levels in Type 2 Diabetic Patients

    OpenAIRE

    M Afkhami - Ardekani; AR Kamali - Ardakani

    2005-01-01

    Introduction: Hyperlipidemia and diabetes are common risk factors for ischemic heart disease, which is the main cause of mortality in diabetic patients. Strict control of blood glucose and other risk factors in diabetics has led to prevention of complications. Garlic has received particular attention for control of blood glucose and decrease in blood lipid levels. At present, several studies have been carried out in order to prove advantages of garlic. Methods: In this study, effects of garsi...

  11. Hyperglycemia (High Blood Glucose)

    Medline Plus

    Full Text Available ... today can fund critical diabetes research and support vital diabetes education services that improve the ... of Hyperglycemia? The signs and symptoms include the following: High blood glucose ...

  12. Hyperglycemia (High Blood Glucose)

    Medline Plus

    Full Text Available ... today can fund critical diabetes research and support vital diabetes education services that improve the lives of ... m.). What are the Symptoms of Hyperglycemia? The signs and symptoms include the following: High blood glucose ...

  13. Hyperglycemia (High Blood Glucose)

    Medline Plus

    Full Text Available ... On Up Treatment & Care Blood Glucose Testing Medication Doctors, Nurses & More Oral Health & Hygiene Women A1C Insulin ... Diabetes Pro: Professional Resources Online We Support Your Doctor Clinical Practice Guidelines Patient Education Materials Scientific Sessions ...

  14. Hyperglycemia (High Blood Glucose)

    Medline Plus

    Full Text Available ... avoid problems associated with hyperglycemia. How Do I Treat Hyperglycemia? You can often lower your blood glucose ... be a serious problem if you don't treat it, so it's important to treat as soon ...

  15. Hyperglycemia (High Blood Glucose)

    Medline Plus

    Full Text Available ... Up Treatment & Care Blood Glucose Testing Medication Doctors, Nurses & More Oral Health & Hygiene Women A1C Insulin Pregnancy ... for Professionals Professional Books Patient Access to Research Student Resources History of Diabetes Resources for School Projects ...

  16. Hyperglycemia (High Blood Glucose)

    Medline Plus

    Full Text Available ... Nephropathy) Gastroparesis Mental Health Step On Up Treatment & Care Blood Glucose Testing Medication Doctors, Nurses & More Oral ... 2 Diabetes Know Your Rights Employment Discrimination Health Care Professionals Law Enforcement Driver's License For Lawyers Food & ...

  17. Hyperglycemia (High Blood Glucose)

    Medline Plus

    Full Text Available ... Nephropathy) Gastroparesis Mental Health Step On Up Treatment & Care Blood Glucose Testing Medication Doctors, Nurses & More Oral Health & Hygiene Women A1C Insulin Pregnancy 8 Tips for Caregivers ...

  18. Hyperglycemia (High Blood Glucose)

    Medline Plus

    Full Text Available ... Care Blood Glucose Testing Medication Doctors, Nurses & More Oral ... someone new is diagnosed. Diabetes causes more deaths a year than breast cancer and AIDS combined. Your gift today will help ...

  19. Hyperglycemia (High Blood Glucose)

    Medline Plus

    Full Text Available ... Hispanic Heritage Month African American Programs Latino Programs Asian Americans, Native Hawaiians and Pacific Islanders American Indian/ ... High blood glucose happens when the body has too little insulin or when the body can't ...

  20. Hyperglycemia (High Blood Glucose)

    Medline Plus

    Full Text Available ... Disease (Nephropathy) Gastroparesis Mental Health Step On Up Treatment & Care Blood Glucose Testing Medication Doctors, Nurses & More ... us get closer to curing diabetes and better treatments for those living with diabetes. Other Ways to ...

  1. Hyperglycemia (High Blood Glucose)

    Medline Plus

    Full Text Available ... Complications DKA (Ketoacidosis) & Ketones Kidney Disease (Nephropathy) Gastroparesis Mental Health Step On Up Treatment & Care Blood Glucose ... Find Your Local Office Find your local diabetes education program Calendar of Events Wellness Lives Here Drive ...

  2. Hyperglycemia (High Blood Glucose)

    Medline Plus

    Full Text Available ... the urine Frequent urination Increased thirst Part of managing your diabetes is checking your blood glucose often. ... Blog Online Community Site Menu Are You at Risk? Diagnosis Lower Your Risk Risk Test Alert Day ...

  3. Hyperglycemia (High Blood Glucose)

    Medline Plus

    Full Text Available ... the urine Frequent urination Increased thirst Part of managing your diabetes is checking your blood glucose often. ... events, such as eating breakfast, take on exaggerated importance. It's a world where a person needs a ...

  4. Human blood glucose dynamics

    OpenAIRE

    Rahaghi, Farbod N.

    2007-01-01

    The control of blood glucose concentration has become central to the prevention of morbidity in diabetes. Currently sensors are becoming available to make available near continuous measurements of tissue glucose concentrations. Frequently measured values provide an opportunity to analyze the dynamics of these measurements in addition to statistical analysis. The dynamics can be used to verify sensor validity, to provide a physiologic control target, and serve as a tool to diagnose and monitor...

  5. Hyperglycemia (High Blood Glucose)

    Medline Plus

    Full Text Available ... In Memory In Honor Become a Member En Español Type 1 Type 2 About Us Online Community ... Page Text Size: A A A Listen En Español Hyperglycemia (High Blood Glucose) Hyperglycemia is the technical ...

  6. Hyperglycemia (High Blood Glucose)

    Medline Plus

    Full Text Available ... produces daily around 4:00 a.m. to 5:00 a.m.). What are the Symptoms of Hyperglycemia? The signs and symptoms include the following: High blood glucose High levels of sugar in the urine Frequent urination Increased ...

  7. Hyperglycemia (High Blood Glucose)

    Medline Plus

    Full Text Available ... Complications DKA (Ketoacidosis) & Ketones Kidney Disease (Nephropathy) Gastroparesis Mental Health Step On Up Treatment & Care Blood Glucose Testing ... html Learn More Join Ryan Reed & Drive Your Health Access tools to help actively manage your diabetes. ... .survey-alert-wrap').remove(); if (data.submitSurveyResponse.success == 'false') { $('.survey-form'). ...

  8. Blood glucose monitoring.

    Science.gov (United States)

    Davey, Sarah

    2014-06-10

    I found the CPD article on blood glucose monitoring and management in acute stroke care interesting and informative. As I am a mental health nursing student, my knowledge of chronic physical conditions is limited, so I learned a lot. PMID:24894257

  9. Hyperglycemia (High Blood Glucose)

    Medline Plus

    Full Text Available ... Size: A A A Listen En Español Hyperglycemia (High Blood Glucose) Hyperglycemia is the technical term for ... diabetes. Learn More: Stories of Courage, Love and Resilience - 2016-08-blog.html Learn More Stories of ...

  10. How to monitor blood glucose.

    Science.gov (United States)

    Dunning, Trisha

    2016-01-27

    Rationale and key points Capillary blood glucose monitoring is an essential component of diabetes care. Blood glucose tests provide important information about how the body is controlling blood glucose metabolism, and the effect of glucose-lowering medicines, illness and stress. ▶ The nurse should consider the rationale for testing blood glucose each time they perform a test, and reflect on the result, taking into consideration the patient's blood glucose target range and recommended care guidelines. ▶ Blood glucose testing times and testing frequency should be planned to suit the glucose-lowering medicine regimen and the clinical situation. Reflective activity Clinical skills articles can help update your practice and ensure it remains evidence based. Apply this article to your practice. Reflect on and write a short account of: 1. What you have gained from this article. 2. How this article will influence your practice when monitoring blood glucose. Subscribers can upload their reflective accounts at: rcni.com/portfolio . PMID:26967884

  11. Assessment risk of osteoporosis in Chinese people: relationship among body mass index, serum lipid profiles, blood glucose, and bone mineral density

    Science.gov (United States)

    Cui, Rongtao; Zhou, Lin; Li, Zuohong; Li, Qing; Qi, Zhiming; Zhang, Junyong

    2016-01-01

    Objective The aim of our study was to investigate the relationship among age, sex, body mass index (BMI), serum lipid profiles, blood glucose (BG), and bone mineral density (BMD), making an assessment of the risk of osteoporosis. Materials and methods A total of 1,035 male and 3,953 female healthy volunteers (aged 41–95 years) were recruited by an open invitation. The basic information, including age, sex, height, weight, waistline, hipline, menstrual cycle, and medical history, were collected by a questionnaire survey and physical examination. Serum lipid profiles, BG, postprandial blood glucose, and glycosylated hemoglobin were obtained after 12 hours fasting. BMD in lumbar spine was measured by dual-energy X-ray absorptiometry scanning. Results The age-adjusted BMD in females was significantly lower than in males. With aging, greater differences of BMD distribution exist in elderly females than in males (P7.0 mmol/L was lower than in people with BG of ≤7.0 mmol/L (P<0.001). People with serum high-density lipoprotein cholesterol levels of ≥1.56 mmol/L had a greater prevalence of osteoporosis compared with high-density lipoprotein cholesterol ≤1.55 mmol/L. Logistic regression with odds ratios showed that no association was found among total cholesterol, triglyceride, low-density lipoprotein cholesterol, glycosylated hemoglobin, postprandial blood glucose and BMD. Conclusion The present study further confirmed that factors such as age, sex, weight, BMI, high-density lipoprotein cholesterol, and diabetes are significant predictors of osteoporosis in the Chinese people. PMID:27445467

  12. Clinical trial of Cecropia obtusifolia and Marrubium vulgare leaf extracts on blood glucose and serum lipids in type 2 diabetics.

    Science.gov (United States)

    Herrera-Arellano, A; Aguilar-Santamaría, L; García-Hernández, B; Nicasio-Torres, P; Tortoriello, J

    2004-11-01

    Cecropia obtusifolia and Marrubium vulgare have been widely used in Mexican traditional medicine for the control of type 2 diabetes. In order to evaluate the clinical effect produced by the aqueous extract from these species on type 2 non-controlled diabetes mellitus, a total of 43 outpatients were included. Based on the European NIDDM (policy group) criteria, only patients with poor response to the conventional treatment were selected. All patients maintained their medical treatment and also received a prepared infusion of the dry leaves of the plant treatment for 21 days. In a double-blind manner, the patients were randomly grouped as follows: 22 patients were treated with C. obtusifolia and 21 with M. vulgare. The fasting blood glucose values were reduced by 15.25% on patients treated with C. obtusifolia, while cholesterol and triglycerides were decreased by 14.62% and 42.0%, respectively (ANOVA pvulgare, the plasma glucose level was reduced by 0.64% and cholesterol and triglycerides by 4.16% and 5.78%, respectively. When the results were compared between groups, significant differences in glucose and cholesterol diminution were found. The obtained results showed that the infusion prepared with the leaves of C. obtusifolia (containing 2.99+/-0.14mg of chlorogenic acid/g of dried plant) produced beneficial effects on carbohydrate and lipid metabolisms when it was administered as an adjunct on patients with type 2 diabetes with poor response to conventional medical treatment. PMID:15636168

  13. Blood glucose response to pea fiber

    DEFF Research Database (Denmark)

    Hamberg, O; Rumessen, J J; Gudmand-Høyer, E

    1989-01-01

    Two new fiber types, pea fiber (PF) and sugar beet fiber (BF), were compared with wheat bran (WB) to investigate the effect on postprandial blood glucose and serum insulin responses in normal subjects. The control meal consisted of 150 g ground beef mixed with 50 g glucose and 20 g lactulose. Only...... addition of PF (15 g pure fiber) reduced the area under the incremental blood glucose curve significantly (by 65%, p less than 0.05). None of the fibers affected the area under the insulin-response curve significantly although it was reduced by all fibers. Mouth-to-cecum transit time, assessed by the...

  14. Hyperglycemia (High Blood Glucose)

    Medline Plus

    Full Text Available ... little insulin or when the body can't use insulin properly. What Causes Hyperglycemia? A number of ... enough insulin. Without insulin, your body can't use glucose for fuel, so your body breaks down ...

  15. Hyperglycemia (High Blood Glucose)

    Medline Plus

    Full Text Available ... Us in the Fight for a Cure Your tax-deductible gift today can fund critical diabetes research ... t use glucose for fuel, so your body breaks down fats to use for energy. When your ...

  16. Hyperglycemia (High Blood Glucose)

    Medline Plus

    Full Text Available ... Doctors, Nurses & More Oral Health & Hygiene Women A1C Insulin Pregnancy 8 Tips for Caregivers Health Insurance Health ... glucose happens when the body has too little insulin or when the body can't use insulin ...

  17. Blood Glucose Monitoring Devices

    Science.gov (United States)

    ... Glucose NIH Medline Plus - Diabetes Spotlight FDA permits marketing of first system of mobile medical apps for ... feeds Follow FDA on Twitter Follow FDA on Facebook View FDA videos on YouTube View FDA photos ...

  18. Hyperglycemia (High Blood Glucose)

    Medline Plus

    Full Text Available ... critical diabetes research and support vital diabetes education services that improve the lives of those with diabetes. $ ... glucose level. Cutting down on the amount of food you eat might also help. Work with your ...

  19. Blood glucose in acute stroke

    DEFF Research Database (Denmark)

    Olsen, Tom Skyhøj

    2009-01-01

    Blood glucose is often elevated in acute stroke, and higher admission glucose levels are associated with larger lesions, greater mortality and poorer functional outcome. In patients treated with thrombolysis, hyperglycemia is associated with an increased risk of hemorrhagic transformation of...... infarcts. For a number of years, tight glycemic control has been regarded as beneficial in critically illness, but recent research has been unable to support this notion. The only completed randomized study on glucose-lowering therapy in stroke has failed to demonstrate effect, and concerns relating to the...... risk of inducing potentially harmful hypoglycemia has been raised. Still, basic and observational research is overwhelmingly in support of a causal relationship between blood glucose and stroke outcome and further research on glucose-lowering therapy in acute stroke is highly warranted....

  20. The Effects of Capparis Spinosa Hydroalcoholic Extract on Blood Glucose and Lipids Serum in Diabetic and Normal Male Rats

    OpenAIRE

    M Negahdarizadeh; Mokhtari, M; JM Malekzadeh; J.Mohammadi

    2011-01-01

    Introduction & Objective: Diabetes mellitus is one of the most common endocrine disorders in the world which affects glucose metabolism in the body. Diabetes mellitus is due to lack of insulin secretion and/or failure in insulin action. Researches conducted in the last few decades on plants have reported anti-diabetic properties for some herbs and their traditional use for diabetes treatment. Capparis spinosa is one of these herbs which are used as an anti-diabetic treatment in tribal medici...

  1. Is Low Blood Glucose (Hypoglycemia) Dangerous?

    Science.gov (United States)

    ... Diabetes Sign Up forJoslin Newsletters Is Low Blood Glucose (Hypoglycemia) Dangerous? Low blood glucose or hypoglycemia is one of the most common ... In general, hypoglycemia is defined as a blood glucose level below 70 mg/dl. Low blood glucose ...

  2. Hyperglycemia (High Blood Glucose)

    Medline Plus

    Full Text Available ... Type 2 Diabetes Risk Test Lower Your Risk Healthy Eating Overweight Smoking High Blood Pressure Physical Activity High ... Diabetes Meal Plans Create Your Plate Gluten Free Diets Meal Planning for Vegetarian Diets Cook with Heart- ...

  3. Hyperglycemia (High Blood Glucose)

    Medline Plus

    Full Text Available ... Type 2 Diabetes Risk Test Lower Your Risk Healthy Eating Overweight Smoking High Blood Pressure Physical Activity High ... the Guesswork out of Planning Meals! Find the healthy eating plan that works for you and your diabetes. ...

  4. Hyperglycemia (High Blood Glucose)

    Medline Plus

    Full Text Available ... Student Resources History of Diabetes Resources for School Projects How to Reference Our Site Diabetes Basics Myths ... blood, which can lead to ketoacidosis. Ketoacidosis is life-threatening and needs immediate treatment. Symptoms include: Shortness ...

  5. Hyperglycemia (High Blood Glucose)

    Medline Plus

    Full Text Available ... you avoid problems associated with hyperglycemia. How Do I Treat Hyperglycemia? You can often lower your blood ... record for use in an emergency. How Can I Prevent Hyperglycemia? Your best bet is to practice ...

  6. Hyperglycemia (High Blood Glucose)

    Medline Plus

    Full Text Available ... Professional Books Patient Access to Research Student Resources History of Diabetes Resources for School Projects How to ... blood, which can lead to ketoacidosis. Ketoacidosis is life-threatening and needs immediate treatment. Symptoms include: Shortness ...

  7. Hyperglycemia (High Blood Glucose)

    Medline Plus

    Full Text Available ... 2 Diabetes Risk Test Lower Your Risk Healthy Eating Overweight Smoking High Blood Pressure Physical Activity High ... Complications Neuropathy Foot Complications DKA (Ketoacidosis) & Ketones Kidney Disease (Nephropathy) Gastroparesis Mental Health Step On Up Treatment & ...

  8. Hyperglycemia (High Blood Glucose)

    Medline Plus

    Full Text Available ... 2 Diabetes Risk Test Lower Your Risk Healthy Eating Overweight Smoking High Blood Pressure Physical Activity High ... What Can I Drink? Fruit Dairy Food Tips Eating Out Quick Meal Ideas Snacks Nutrient Content Claims ...

  9. Effect of the hydroalcoholic extract and juice of Prunus divaricata fruit on blood glucose and serum lipids of normal and streptozotocin-induced diabetic rats.

    Science.gov (United States)

    Minaiyan, M; Ghannadi, A; Movahedian, A; Ramezanlou, P; Osooli, F S

    2014-01-01

    Prunus divaricata (Alloocheh) is a small tree cultivating in Iran, Middle East and central Asia. Prunus genus has many species with anti-oxidant, anti-hyperlipidemia and anti-hyperglycemia effects. In the present study the anti-diabetic and anti-hyperlipidemic effects of P. divaricata fruits were examined in normal and streptozotocin (STZ)-induced diabetic rats. Both groups, control and reference rats received normal saline and glibenclamide respectively. Test groups were treated with Prunus freeze dried juice (PFDJ, 200, 400, 800 mg/kg) and Prunus freeze dried extract (PFDE, 100, 200, 400 mg/kg) started at the 3(rd) day of the experiment and continued for 27 days thereafter. Weight changes of animals were checked periodically. Fasting blood glucose (FBG) level as well as serum triglyceride (TG), total cholesterol (TC), high density lipoprotein (HDL) and low density lipoprotein (LDL) cholesterol were determined. Different treatments had no significant effect on body weight increments of normal rats, while in diabetic rats, PFDJ (800 mg/kg) and PFDE (400 mg/kg) opposed with weight loss. In acute phase of experiment (0-8 h of 3(rd) day), none of tested fractions were effective in reducing FBG and serum lipids of normal rats. During the sub-acute phase (13(th) and 30(th) days) however, the greatest test doses of PFDJ (800 mg/kg) and PFDE (400 mg/kg) induced hypoglycema. In diabetic groups, PFDJ and PFDE, at all test doses, could diminish FBG during sub-acute phase of the experiment. In addition, PFDJ and PFDE at most examined doses could diminish TG significantly and they were also effective on cholesterol derivatives in different magnitude. PMID:26339257

  10. Hyperglycemia (High Blood Glucose)

    Medline Plus

    Full Text Available ... and-a1c-hyperglycemia,tips-and-how-tos, In this section Living With Diabetes Treatment and Care Blood ... ensureArray(data.submitSurveyResponse.errors); $.each(surveyErrors, function () { if (this.errorField) { $('input[name="' + this.errorField + '"]').closest('.form-group') . ...

  11. Breakfast, blood glucose, and cognition.

    Science.gov (United States)

    Benton, D; Parker, P Y

    1998-04-01

    This article compares the findings of three studies that explored the role of increased blood glucose in improving memory function for subjects who ate breakfast. An initial improvement in memory function for these subjects was found to correlate with blood glucose concentrations. In subsequent studies, morning fasting was found to adversely affect the ability to recall a word list and a story read aloud, as well as recall items while counting backwards. Failure to eat breakfast did not affect performance on an intelligence test. It was concluded that breakfast consumption preferentially influences tasks requiring aspects of memory. In the case of both word list recall and memory while counting backwards, the decline in performance associated with not eating breakfast was reversed by the consumption of a glucose-supplemented drink. Although a morning fast also affected the ability to recall a story read aloud, the glucose drink did not reverse this decline. It appears that breakfast consumption influences cognition via several mechanisms, including an increase in blood glucose. PMID:9537627

  12. Reusable glucose fiber sensor for measuring glucose concentration in serum

    Institute of Scientific and Technical Information of China (English)

    Cheng-Chih Hsu; Yi-Cheng Chen; Ju-Yi Lee; Chyan-Chyi Wu

    2011-01-01

    We demonstrate a glucose fiber sensor for measuring glucose concentration in serum. High resolution and rapid measurement are achieved through the integration of highly selective enzymes and heterodyne interferometry. The best resolution and response time obtained are 0.14mg/dL and 1.3 s, respectively. The stability of the sensor is also verified by investigating the initial phase variation. Experimental results show that the fiber sensor can be reused more than 10 times.%We demonstrate a glucose fiber sensor for measuring glucose concentration in serum.High resolution and rapid measurement are achieved through the integration of highly selective enzymes and heterodyne interferometry.The best resolution and response time obtained are 0.14 mg/dL and 1.3 s,respectively.The stability of the sensor is also verified by investigating the initial phase variation.Experimental results show that the fiber sensor can be reused more than 10 times.Fiber sensors have attracted considerable attention over the past two decades.Various kinds of fiber sensors have been proposed for measnring specific chemical concentrations[1-8].Most previously reported methods[1-5] involved measuring the variations in fluorescence intensity[2-4] or transmitted light[3,4].Hence,avoiding the inflnence of snrrounding light and the use of expensive photon detection equipment are important requirements.Furthermore,procedures for manufacturing optical biosensors are complicated[3] and qualitv is difficult to control[4]..

  13. Alterations in the blood glucose, serum lipids and renal oxidative stress in diabetic rats by supplementation of onion (Allium cepa. Linn)

    OpenAIRE

    Bang, Mi-Ae; Kim, Hyeon-A; Cho, Young-Ja

    2009-01-01

    This study examined the anti-diabetic effect of onion (Allium cepa. Linn) in the streptozotocin (STZ)-induced diabetic rats. Male Sprague-Dawley rats were divided into normal rats fed control diet or supplemented with onion powder (7% w/w) and diabetic rats fed control diet or supplemented with onion powder. Diabetes was induced by a single injection of STZ (60 mg/kg, ip) in citrate buffer. The animals were fed each of the experimental diet for 5 weeks. Blood glucose levels of rats supplement...

  14. What is a normal blood glucose?

    Science.gov (United States)

    Güemes, Maria; Rahman, Sofia A; Hussain, Khalid

    2016-06-01

    Glucose is the key metabolic substrate for tissue energy production. In the perinatal period the mother supplies glucose to the fetus and for most of the gestational period the normal lower limit of fetal glucose concentration is around 3 mmol/L. Just after birth, for the first few hours of life in a normal term neonate appropriate for gestational age, blood glucose levels can range between 1.4 mmol/L and 6.2 mmol/L but by about 72 h of age fasting blood glucose levels reach normal infant, child and adult values (3.5-5.5 mmol/L). Normal blood glucose levels are maintained within this narrow range by factors which control glucose production and glucose utilisation. The key hormones which regulate glucose homoeostasis include insulin, glucagon, epinephrine, norepinephrine, cortisol and growth hormone. Pathological states that affect either glucose production or utilisation will lead to hypoglycaemia. Although hypoglycaemia is a common biochemical finding in children (especially in the newborn) it is not possible to define by a single (or a range of) blood glucose value/s. It can be defined as the concentration of glucose in the blood or plasma at which the individual demonstrates a unique response to the abnormal milieu caused by the inadequate delivery of glucose to a target organ (eg, the brain). Hypoglycaemia should therefore be considered as a continuum and the blood glucose level should be interpreted within the clinical scenario and with respect to the counter-regulatory hormonal responses and intermediate metabolites. PMID:26369574

  15. Prediction Methods for Blood Glucose Concentration

    DEFF Research Database (Denmark)

    Freckmann et al. discusses performance metrics used to characterize the accuracy of continuous glucose measurement devices. This topic is highly relevant for prediction models since many of them rely on the data given by the continuous sensors which are previously calibrated with blood glucose meter...... on the net effect of meals on the blood glucose concentration. By assuming that all major unexplained glycemic excursions can be vi Preface attributed to oral glucose ingestion, a meal vector is estimated which significantly improves the mathematical model. Results are shown on three patients during...... intrasubject variability by using the concept of interval pre-dictions. Instead of predicting a single blood glucose value in the future, a whole solution envelope is determined. With the presented theory it can be guaranteed that the real value is always inside of the envelope and moreover the envelope...

  16. Nanostructured gold electrodes for determination of glucose in blood

    Czech Academy of Sciences Publication Activity Database

    Farka, Z.; Juřík, T.; Kovář, D.; Podešva, Pavel; Foret, František; Skladal, P.

    Brno: Ústav analytické chemie AV ČR, v. v. i, 2015 - (Foret, F.; Křenková, J.; Drobníková, I.; Klepárník, K.), s. 54-57 ISBN 978-80-904959-3-7. [CECE 2015. International Interdisciplinary Meeting on Bioanalysis /12./. Brno (CZ), 21.09.2015-23.09.2015] Institutional support: RVO:68081715 Keywords : monitoring of glucose * glucometers * non-enzymatic glucose determination * human blood serum Subject RIV: CB - Analytical Chemistry, Separation http://www.ce-ce.org/CECE2015/CECE%202015%20proceedings_full.pdf

  17. Nanostructured gold electrodes for determination of glucose in blood

    Czech Academy of Sciences Publication Activity Database

    Farka, Z.; Juřík, T.; Kovář, D.; Podešva, Pavel; Foret, František; Skladal, P.

    Brno : Ústav analytické chemie AV ČR, v. v. i, 2015 - (Foret, F.; Křenková, J.; Drobníková, I.; Klepárník, K.), s. 54-57 ISBN 978-80-904959-3-7. [CECE 2015. International Interdisciplinary Meeting on Bioanalysis /12./. Brno (CZ), 21.09.2015-23.09.2015] Institutional support: RVO:68081715 Keywords : monitoring of glucose * glucometers * non-enzymatic glucose determination * human blood serum Subject RIV: CB - Analytical Chemistry, Separation http://www.ce-ce.org/CECE2015/CECE%202015%20proceedings_full.pdf

  18. Non-Invasive Optical Blood Glucose Measurement

    Directory of Open Access Journals (Sweden)

    Megha C.Pande

    2013-07-01

    Full Text Available The method for noninvasively blood glucose monitoring system is discussed in this paper. Lot of research work has been done in developing the device which is completely noninvasive to avoid the pros & cons because of frequent pricking. In this paper we are trying to analyze the noninvasive blood glucose measurement study in the near infrared region which is the most suitable region for blood glucose measurement. For this purpose we use a technique which is similar to pulseoximetry based on near infrared spectrometry .An infrared light of particular wavelength is passed through fingertip containing an arterial pulse component are derived,thus minimizing influences of basal components such as resting blood volume,skin, muscle and bone.

  19. Alterations of serum concentrations of thyroid hormones and sex hormone-binding globulin, nuclear binding of tri-iodothyronine and thyroid hormone-stimulated cellular uptake of oxygen and glucose in mononuclear blood cells from patients with non-thyroidal illness

    DEFF Research Database (Denmark)

    Kvetny, J; Matzen, L

    1990-01-01

    Nuclear tri-iodothyronine (T3) binding and thyroid hormone-stimulated oxygen consumption and glucose uptake were examined in mononuclear blood cells from patients with non-thyroidal illness (NTI) in which serum T3 was significantly (P less than 0.05) depressed (0.62 +/- 0.12 (S.D.) nmol/l) compar...

  20. Predicting Mortality of Critically Ill Patients by Blood Glucose Levels

    Directory of Open Access Journals (Sweden)

    Byung Sam Park

    2013-10-01

    Full Text Available BackgroundThe aim of this study is to observe the outcome of critically ill patients in relation to blood glucose level at admission and to determine the optimal range of blood glucose at admission predicting lower hospital mortality among critically ill patients.MethodsWe conducted a retrospective cohort study of a total 1,224 subjects (males, 798; females, 426 admitted to intensive care unit (ICU from 1 January 2009 to 31 December 2010. Blood glucose levels at admission were categorized into four groups (group 1, <100 mg/dL; group 2, 100 to 199 mg/dL; group 3, 200 to 299 mg/dL; and group 4, ≥300 mg/dL.ResultsAmong 1,224 patients, 319 patients were already known diabetics, and 296 patients died in ICU. Five hundred fifty-seven subjects received insulin therapy, and 118 received oral hypoglycemic agents. The overall mortality rate was 24.2% (296 patients. The causes of death and mortality rates of diabetic patients were not different from nondiabetic subjects. The mortality curve showed J shape, and there were significant differences in mortality between the groups of blood glucose levels at admission. Group 2 had the lowest mortality rate (P<0.05.ConclusionThese results suggest that serum glucose levels upon admission into ICU is associated with clinical outcomes in ICU patients. Blood glucose level between 100 and 199 mg/dL at the time of ICU admission could predict lower hospital mortality among critically ill patients.

  1. Effect and potential mechanism of action of sea cucumber saponins on postprandial blood glucose in mice.

    Science.gov (United States)

    Fu, Xueyuan; Wen, Min; Han, Xiuqing; Yanagita, Teruyoshi; Xue, Yong; Wang, Jingfeng; Xue, Changhu; Wang, Yuming

    2016-06-01

    Postprandial blood glucose control is the major goal in the treatment of diabetes. Here, we investigated the effect of sea cucumber saponins (SCSs) on postprandial blood glucose levels. SCS inhibited yeast as well as rat intestinal α-glucosidase activity in a dose-dependent manner and showed better inhibition of yeast α-glucosidases compared to the positive control. Further studies were performed using ICR mice treated with SCS and starch or SCS alone by oral gavage. Unexpectedly, SCS increased postprandial blood glucose levels a short time (1 h) after oral gavage. The serum corticosterone (CORT) level showed a consistent correlation with glucose levels. In vitro experiments confirmed that SCS treatment increased the secretion of CORT in the Y1 adrenal cell line. Overall, these studies demonstrated that SCS gavage could inhibit α-glucosidase activity but cannot attenuate postprandial blood glucose level within short time periods. The underlying mechanisms are probably related to increased serum CORT levels. PMID:26932154

  2. Recent developments in blood glucose sensors

    Directory of Open Access Journals (Sweden)

    Hui-Chen Wang

    2015-06-01

    Full Text Available Diabetes has recently become a leading cause of death worldwide. To date, although there is no means to cure or prevent diabetes, appropriate medication and blood sugar monitoring can enhance treatment efficiency, alleviate the symptoms, and diminish the complications of the condition. This review article deals with current growth areas in the market for blood glucose sensors and possible future alternatives, which are generally considered to be the point sample test and the continuous glucose monitor (CGM. Most glucose sensors are enzyme-based, whereas others are enzyme-free. The former class is sensitive and some products are extensively employed for daily self-sensing and in hospital environments as reliable diagnostic tools. The latter class, particularly the boronic acid fluorescent sensor, is facile and extremely promising. Practicality demands that all types of sensors offer accuracy, specificity, and real-time detection.

  3. Random blood glucose testing in dental practice

    DEFF Research Database (Denmark)

    Barasch, Andrei; Safford, Monika M; Qvist, Vibeke;

    2012-01-01

    The prevalence of diabetes mellitus (DM) has been increasing. Instances of patients' not having received a diagnosis have been reported widely, as have instances of poor control of DM or prediabetes among patient's who have the disease. These facts indicate that blood glucose screening is needed....

  4. 成人身体成分与血脂、血糖关系的探讨%Analysis of the relationship between body composition and fasting serum lipids, fasting blood glucose in adult

    Institute of Scientific and Technical Information of China (English)

    李秋桂; 姬芳勤

    2014-01-01

    目的:分析成人身体成分与血脂、血糖指标之间的关系方法选择成年体检者490名,排除严重心脑肝肾疾病,肿瘤患者,急性炎症患者及长期服药者等。通过Biospace InBody 220人体成分分析仪对身体水分、蛋白质、无机盐、体脂肪、体重、骨骼肌肉量、体质指数、体脂百分比、腰臀脂肪比等指标进行测定。同时测定空腹血糖、血脂指标。所有指标的测定均由我院医护人员经过统一培训后进行操作。结果男女性别年龄无差异(p>0.05)。除体脂肪外,男女性别间身体水分、蛋白质、无机盐、体重、骨骼肌肉量、体质指数、体脂百分比、腰臀脂肪比均有显著差异(均p<0.001)。蛋白质、体脂肪、体重、骨骼肌肉量、体质指数、体脂百分比、腰臀脂肪比与空腹血糖呈正相关(均p<0.05)。身体水分、蛋白质、无机盐、体脂肪、体重、骨骼肌肉量、体质指数、体脂百分比、腰臀脂肪比均可对血脂(总胆固醇、甘油三酯)产生影响(均p<0.05)。剔除性别因素后,对身体成分与血糖、血脂各指标进行偏相关分析,体脂肪、体重、体质指数、体脂百分比、腰臀脂肪比仍然与血糖、总胆固醇、甘油三酯呈正相关(均p<0.05)。结论成人身体成分与血糖、血脂指标密切相关。通过身体成分的分析,可尽早预防代谢相关性疾病。%Objective To analyze the relationship between body composition and fasting serum lipids, fasting blood glucose in adult. Methods Select 490 adult, eliminate the subjects who has the serious cardiovascular diseases, liver diseases , kidney diseases, cancer and the subjects with acute inflammation and long term medication etc. Body moisture, protein, inorganic salt, fat, skeletal muscle weight, body mass index, percentage of body fat, waist and hip fat ratio was determined by Biospace InBody 220. Measure the

  5. Blood glucose and nocturnal blood pressure in African and caucasian men: the SABPA study

    OpenAIRE

    Lammertyn, Leandi; Schutte, Aletta Elisabeth; Schutte, Rudolph

    2011-01-01

    To investigate the relationship between nocturnal blood pressure and chronically elevated blood glucose to determine if these elevated blood glucose concentrations contribute to a non-dipping blood pressure, especially in high-risk groups such as Africans.

  6. Blood serum atherogenicity and coronary artery calcification.

    Science.gov (United States)

    Sobenin, Igor A; Myasoedova, Veronica A; Anisimova, Elena V; Pavlova, Xenia N; Möhlenkamp, Stefan; Schmermund, Axel; Seibel, Rainer; Berenbein, Sina; Lehmann, Nils; Moebus, Susanne; Jöckel, KarlHeinz; Orekhov, Alexander N; Erbel, Raimund

    2014-01-01

    The phenomenon of blood serum atherogenicity was described as the ability of human serum to induce lipid accumulation in cultured cells. The results of recent two-year prospective study in asymptomatic men provided the evidence for association between the changes in serum atherogenicity and dynamics of carotid intima-media thickness progression. The present study was undertaken to test the hypothesis that blood serum atherogenicity and its changes in dynamics may be associated with accumulation of coronary calcium in subclinical atherosclerosis. It was performed in 782 CHD-free participants of The Heinz Nixdorf RECALL (Risk Factors, Evaluation of Coronary Calcium and Lifestyle) Study, in whom blood samples have been taken at the baseline and at the end of 5-year follow-up. Opposite to the previous findings, the changes in serum atherogenicity did not correlate neither with the extent of coronary artery calcification, nor with the changes in Agatston CAC score. There was a moderate but significant rise in serum atherogenicity after 5-year followup period, and the same dynamics was observed for Agatston CAC score, but not for convenient lipid-related risk factors. The absence of association of the changes in serum atherogenicity with the changes in Agatston CAC score, along with previous findings, provides a point of view that serum-induced intracellular cholesterol accumulation is not related to the processes of calcium deposition in arterial wall, since the last one reflects the progression of already existing subclinical atherosclerotic lesions. PMID:24533940

  7. 急性缺血性脑血管病患者血清内皮素以及血糖、肌酐、尿素氮水平的研究%Study on levels of serum endothelin, blood glucose, creatinine and blood urea nitrogen in patients with acute ischemic cerebrovascular disease

    Institute of Scientific and Technical Information of China (English)

    赵华头; 汪杏; 徐旭然; 严婷婷

    2012-01-01

    目的 研究急性缺血性脑血管病患者血清内皮素以及血糖、肌酐、尿素氮水平变化规律,探讨神经功能缺损程度与血清内皮素水平之间的关系.方法 对照分析急性缺血性脑血管病患者与同期非神经系统疾病患者的血清内皮素以及血糖、肌酐、尿素氮水平.结果 急性脑梗死组患者血糖以及ET-1水平明显高于对照组,其他指标差异无统计学意义.血清ET-1水平与神经功能缺损程度具有显著的正相关性.结论防治胰岛素抵抗可以作为防治急性缺血性脑血管病的有效措施.血清ET-1水平可以作为急性缺血性脑血管病的敏感监测指标,对于预测患者预后具有重要意义.%Objective To study levels of the acute ischemic cerebrovascular disease in patients with serum endothelin and blood glucose,creatinine,blood urea nitrogen,and explore the relationship between the degree of neurological deficit and serum endothelin levels. Methods The serum endothelin and levels of blood sugar,creatinine,and blood urea nitrogen between patients with acute ischemic cerebrovascular disease and patients with non - nervous system disease were comparatively analyzed. Results The blood glucose and ET- 1 levels of patients with acute cerebral infarction were significantly higher than those of patients in the control group. The differences of the other indicators were not statistically significant. There was significant positive correlation between serum ET- 1 levels and the degree of neurological deficits. Conclusion The prevention and treatment of insulin resistance can be used as an effective measure to prevent and treat acute ischemic cerebrovascular disease. Serum ET- 1 levels can be used as sensitive indicators for monitoring of a-cute ischemic cerebrovascular disease,and of great significance for predicting the prognosis of patients.

  8. Serum glucose concentration and lipid profile in racing horses

    Directory of Open Access Journals (Sweden)

    S. A. Hasso

    2012-01-01

    Full Text Available The aim of the present work was to evaluate serum glucose concentration and lipid profile in racing horses in Iraq. Blood samples were collected from the jugular vein of 92 clinically healthy racing horses (males and females, Arabian and Cross bred, 2-8 years old at Equestrian Club, Baghdad. Investigations included serum measurements of glucose (sg and lipid profile parameters; total cholesterol (TC, Triglyceride (TG, high density lipoprotein- cholesterol (HDL-C, low density lipoprotein-cholesterol (LDL-C, very low density lipoprotein-cholesterol (VLDL-C and the atherogenic ratio (LDL-C/HDL-C, in order to register the normal ranges and mean values of these measured parameters in Iraqi healthy racing horses. Results showed that the range and mean values ± standard error of sg were: 33.3 – 6.71 mmol/l and 5.17±0.07 mmol/l, respectively, whereas the TC was 2.07 – 4.22 mmol/l and 3.01±0.05 mmol/l, TG 0.6 – 1.47 mmol/l and 1.06±0.02 mmol/l, HDL-C 0.93 – 2.25 mmol/l and 1.50±0.03 mmol/l, LDL-C 0.10 – 2.12 mmol/l and 0.91±0.04 mmol/l, VLDL-C 0.31 -0.67 mmol/l and 0.55±0.02 mmol/l, respectively and the atherogenic ratio 0.66±0.03. The data present reference values and mean ± SE for sg and lipid profile parameters in healthy racing horses in Baghdad.

  9. 内源性n-3多不饱和脂肪酸对fat-1转基因小鼠血糖血脂的影响%Effects of Endogenous n-3 Polyunsaturated Fatty Acids (PUFAs) on Blood Glucose and Serum Lipid in fat-1 Transgenic Mouse Model

    Institute of Scientific and Technical Information of China (English)

    周飞; 张晓宏; 邹祖全; 张才乔

    2015-01-01

    利用fat-1转基因小鼠模型,研究内源性n-3多不饱和脂肪酸(n-3 PUFAs)的血糖血脂调节作用.采用fat-1转基因小鼠和C57BL/6野生型小鼠喂食高n-6、低n-3 PUFAs的标准配方饲料4周,然后2组小鼠给予高糖饮液自由饮用4周,每周测体重.第8周末,取血离心测血糖、胰岛素、甘油三脂(TG)、胆固醇(TC)、高密度脂蛋白(HDL-C)、低密度脂蛋白(LDL-C)水平.结果表明:fat-1转基因小鼠体重增加幅度、空腹血糖值、血清胰岛素、胰岛素抵抗指数、血清TC、TG、HDL-C、LDL-C水平明显低于野生型小鼠(P<0.05).认为n-3 PUFAs能抑制体重增长,降低小鼠血糖、血脂和胰岛素抵抗,起到调节血糖、血脂的作用.%In this study, we investigate the effects of endogenous n-3 PUFAs on blood glucose and serum lipid in fat-1 transgenic mouse model. The transgenic and wild type mice are maintained on a 10%corn oil diet which has high ratio of n-6/n-3 polyunsaturated fatty acids for 4 weeks. Then make them drink high sugar pinocytosis freely for another four weeks. On the eighth weekend, serum glucose, insulin, serum triglyceride (TG), serum total cholesterol (TC), serum high-density lipoprotein cholesterol (HDL-C), serum low-density lipoprotein cholesterol (LDL-C) levels of blood serum are tested after blood collection and centrifugation. The weight gain rate, fasting blood sugar and serum insulin levels, insulin resistance index, serum TC, TG, HDL-C, LDL-C of fat-1 transgenic mice are found to be significantly lower than those of wild type mice (P<0.05). The tests indicate that n-3 polyunsaturated fatty acids can reduce the blood glucose and serum lipid, which may play a vital role in regulating blood lipid and decreasing insulin resistance.

  10. Blood glucose control and monitoring in the critically ill

    NARCIS (Netherlands)

    R.T.M. van Hooijdonk

    2015-01-01

    This thesis deals with blood glucose control and blood glucose monitoring in intensive care unit (ICU) patients: two important aspects of care for and monitoring of critically ill patients. While the precise targets of blood glucose control in ICU patients remain a matter of debate, currently many,

  11. Blood Glucose Measurement Using Bioimpedance Technique

    OpenAIRE

    Kamat, D. K.; Dhanashri Bagul; Patil, P. M.

    2014-01-01

    Bioimpedance measurement is gaining importance in wide field of bioresearch and biomedical systems due to its noninvasive nature. Noninvasive measurement method is very important to decrease infection and physical injuries which result due to invasive measurement. This paper presents basic principle of bioimpedance along with its application for blood glucose analysis and effect of frequency on impedance measurement. Input from bioimpedance sensor is given to amplifier and signal conditioner ...

  12. Efficacy of Tribulus Terrestris Extract on the Serum Glucose and Lipids of Women with Diabetes Mellitus

    Science.gov (United States)

    Samani, Nasrin Babadai; Jokar, Azam; Soveid, Mahmood; Heydari, Mojtaba; Mosavat, Seyed Hamdollah

    2016-01-01

    Background: Considering folkloric use of Tribulus terrestris (T. terrestris) in diabetes and proven anti-hyperglycemic and anti-hyperlipidemic effects of T. terrestris in animal studies, we aimed to evaluate the efficacy of the hydro alcoholic extract of T. terrestris on the serum glucose and lipid profile of women with diabetes mellitus. Methods: Ninety-eight diabetic women were randomly allocated to receive the T. terrestris (1000 mg/day) or placebo for three months. The patients were evaluated in terms of the fasting blood glucose, 2-hour postprandial glucose, glycosylated hemoglobin and lipid profile. Results: T. terrestris showed a significant blood glucose lowering effect in diabetic women compared to placebo (Pdiabetic women.

  13. Serum Visfatin Levels, Adiposity and Glucose Metabolism in Obese Adolescents

    OpenAIRE

    Taşkesen, Derya; Kirel, Birgül; Us, Tercan

    2012-01-01

    Objective: Visfatin, an adipokine, has insulin-mimetic effects. The main determinants of both the production and the physiologic role of visfatin are still unclear. The aim of this study is to determine the relation of serum visfatin to adiposity and glucose metabolism. Methods: 40 pubertal adolescents (20 females, 20 males; age range: 9-17 years) with exogenous obesity and 20 age- and sex-matched healthy adolescents (10 females, 10 males) were enrolled in the study. Oral glucose tolerance te...

  14. Prediction Methods for Blood Glucose Concentration

    DEFF Research Database (Denmark)

    “Meta-Learning Based Blood Glucose Predictor for DiabeticSmartphone App” by Valeriya Naumova et al. demonstrates how a highly sophisticated glucose prediction model can be ported from a development language running on a PC to a format such that it can be used conveniently by the patients. A unique...... Continuous EEG Monitoring” by Claus Borg Juhl et al. shows how to use EEG signals to predict upcoming hypoglycemic situations in real-time by employing artificial neural networks. The results of a 30-day long clinical study with the implanted device and the developed algorithm are presented. The chapter......-Based Linear Multistep Predictors” by Marzia Cescon et al. uses data-based methods to develop individualized prediction models. The model can be considered as a combination of physiological models to precompute the rate of appearance of injected insulin and ingested carbohydrates in the bloodstream and of data...

  15. Blood Glucose Measurement Using Bioimpedance Technique

    Directory of Open Access Journals (Sweden)

    D. K. Kamat

    2014-01-01

    Full Text Available Bioimpedance measurement is gaining importance in wide field of bioresearch and biomedical systems due to its noninvasive nature. Noninvasive measurement method is very important to decrease infection and physical injuries which result due to invasive measurement. This paper presents basic principle of bioimpedance along with its application for blood glucose analysis and effect of frequency on impedance measurement. Input from bioimpedance sensor is given to amplifier and signal conditioner AD5933. AD5933 is then interfaced with microcontroller LPC1768 using I2C bus for displaying reading on LCD. Results can also be stored in database using UART interface of LPC1768.

  16. Detection accuracy of three glucose meters estimated by capillary blood glucose measurements compared with venous blood evaluated by the diabetes unit of the Hospital Evangélico de Curitiba, Brazil

    Directory of Open Access Journals (Sweden)

    Camacho SL

    2012-05-01

    Full Text Available Mirnaluci Paulino Ribeiro Gama, Camile Fiorese Cruzeta, Ana Carolina Ossowski, Marina Rech Bay, Mariella Muller Michaelis, Stênio Lujan CamachoEndocrinology and Diabetes Service, Hospital Universitário Evangélico de Curitiba, BrazilObjective: To compare capillary blood glucose measurements between three different glucose meters and with the serum glucose values of inpatients at the diabetes unit of Hospital Universitário Evangélico de Curitiba, Brazil.Materials and methods: A total of 132 non-intensive care unit patients admitted for medical and surgical pathologies were evaluated. All patients reported a previous diagnosis of diabetes mellitus, were under 60 years of age, had no hematocrit alterations, remained hemodynamically stable during the time of data collection, and were given no ascorbic acid, acetaminophen, dopamine, or mannitol during follow-up. Capillary and serum blood glucose samples were collected simultaneously by finger-stick and venipuncture 2 hours after lunch, by the same observer, who was blinded to the serum glucose results. First, between July and November 2009, capillary glucose levels were measured using the blood glucose meters OneTouch SureStep® and MediSense Optium®. Between November 2009 and February 2010, capillary blood glucose levels were measured on the glucose meters OneTouch SureStep and Optium Xceed®. The capillary glucose readings were analyzed between meters and also in relation to the serum blood glucose values by the t-test for paired samples and the Mood two-sample test.Results: The patients’ mean age was 50.45 years. The blood glucose means obtained using the meters OneTouch SureStep, MediSense Optium, and Optium Xceed were, respectively, 183.87 mg/dL, 178.49 mg/dL, and 192.73 mg/dL, and the mean for the serum glucose values was 174.58 mg/dL. A significant difference was found between the capillary measurements taken by the glucose meters and the serum glucose measurements (P < 0.05, and no

  17. A Survey On Ionic And Metabolite Factors Of Blood Serum In Kutum (Rutilus frisii kutum

    Directory of Open Access Journals (Sweden)

    Afkhami Majid

    2014-10-01

    Full Text Available In this study, ionic parameters and metabolite factors (cholesterol, total protein, and glucose of serum and their interrelationships were detected in 48 specimens of kutum (Rutilus frisii kutum captured during spawning migration. Blood sampling was conducted by cutting the caudal peduncle of each sample, and blood was collected into heparinized and sterile capillary glass tubes.

  18. Relationship of endothelial nitric oxide synthase gene polymorphism with blood pressure,lipid profile and blood glucose level

    Institute of Scientific and Technical Information of China (English)

    2008-01-01

    To study the relationship of the polymorphism of endothelial nitric oxide synthase(eNOS)gene and blood pressure,lipid profiles and blood glucose level.By using PCR-RFLP,the eNOS Glu298Asp gene polymorphism was detected in 184 patients with essential hypertension and 196 matched healthy individuals with normal blood pressure.Taking into account eNOS Glu298Asp polymorphisms,the relationship of blood pressure with triglycerides(TG),total cholesterol(TC),high density lipoprotein(HDL),low density lipoprotein(LDL)and blood glucose level was analyzed.The distribution of eNOS Glu298Asp polymorphism had no significant difference between different blood pressure groups and gender groups,but there was a significant difference between different age groups,diastolic blood pressure groups or BMI groups(P<0.05).Asp/Asp genotype significantly increased the risk of hypertension in individuals with serum TC above 5.4 mmol/L(P=0.03,OR=2.65).eNOSGlu298Asp polymorphism and serum lipid could synergistically modulate the blood pressure,eNOS Asp/Asp genotype could significantly increase the risk of hypertension in individuals with serum TC over 5.4 mmol/L,eNOS Glu298Asp in combination with serum TC could be used to predict the risk of hypertension.

  19. Effects of breakfast with different calorigenic amounts on blood glucose, insulin and glucagon levels

    Institute of Scientific and Technical Information of China (English)

    HUANG Su-xia(黄素霞); FU Yun-xing(傅运兴); WANG Hui-ming(王慧铭); GIANG Sylvia

    2003-01-01

    This study was aimed to investigate the relationship between breakfast and serum glucose, insulin and glucagon concentrations in order to establish a model breakfast appropriate for Chinese. Twenty-four volunteers were randomly assigned to four study groups: high carbohydrate breakfast, high fat and protein breakfast, the typical breakfast and fasting. Each subject had serum and urine samples collected while fasting and at 1,2 and 3.5 hours following the meal. The concentration of serum glucose, insulin and glucagon was measured. The levels of serum glucose in group A,B and C differed significantly at 1 and 2 hour after meal compared to those at fasting (P<0.05). The serum glucose in group A increased insignificantly after meal. The serum insulin levels were in group A,B and C significant different compared with control group(P<0.05).Those peaked at 1 hour after meal ,with group C rising the furthest. Compared with the fasting group, the serum glucagons rose and maintained the increase after breakfast in group A,B and C (P<0.05).The data suggested that various diets with different calorigenic amounts increased hormone concentration to various extents. We found that a breakfast rich in carbohydrates could maintain proper blood glucose level.

  20. Effects of breakfast with different calorigenic amounts on blood glucose, insulin and glucagon levels

    Institute of Scientific and Technical Information of China (English)

    黄素霞; 傅运兴; 王慧铭; GIANGSylvia

    2003-01-01

    This study was aimed to investigate the relationship between breakfast and serum glucose, insulin and glueagon concentrations in order to establish a model breakfast appropriate for Chinese. Twenty-four vol-unteers were randomly assigned to four study groups: high carbohydrate breakfast, high fat and protein break-fast, the typical breakfast and fasting. Each subject had serum and urine samples collected while fasting andat 1,2 and 3.5 hours following the meal. The concentration of serum glucose, insulin and glucagon was mea-sured. The levels of serum glucose in group A, B and C differed significantly at 1 and 2 hour after meal com-pared to those at fasting ( P < 0.05) . The serum glucose in group A increased insignificantly after meal. The serum insulin levels were in group A, B and C significant different compared with control group( P < 0.05) .Those peaked at 1 hour after meal ,with group C rising the furthest. Compared with the fasting group, the se-rum glueagons rose and maintained the increase after breakfast in group A, B and C (P < 0.05).The data suggested that various diets with different ealorigenie amounts increased hormone concentration to various extents. We found that a breakfast rich in carbohydrates could maintain proper blood glucose level.

  1. Association of serum uric acid with different levels of glucose and related factors

    Institute of Scientific and Technical Information of China (English)

    YUAN Hui-juan; YANG Xu-guang; SHI Xiao-yang; TIAN Rui; ZHAO Zhi-gang

    2011-01-01

    Background Previous studies have demonstrated that serum uric acid (UA) is an independent predictor of incident type 2 diabetes mellitus (T2DM) in general populations. This study aimed to investigate specific characteristics of UA and its relationship between UA and blood glucose and other risk factors in the Chinese population.Methods A total of 946 subjects were included in this study. UA, glucose, insulin, fractional excretion of UA (FEua),creatinine clearance rate (Ccr), hemoglobin A1c (HbA1c), fructosamine (FA), blood pressure and lipids were studied and also reexamined after the patients underwent two weeks of combined therapeutics.Results UA levels were the highest in subjects with impaired glucose regulation (IGR), followed by subjects with normoglycemia (NGT) and finally by subjects with T2DM. The level of the 2-hour postprandial insulin and the area under the curve for insulin (AUCins) showed a similar tendency. The UA levels initially increased with increasing fasting blood glucose (FBG) and postprandial blood glucose (PPBG) levels, up to 7 mmol/L and 10 mmol/L, respectively, and thereafter decreased at higher FBG and PPBG levels. Compared with subjects in the lower serum UA quartile, subjects in the upper quartile of serum UA levels had higher weights, triglyceride levels, and creatinine levels as well as lower Ccr and FEua levels. Compared with women's group, UA levels were higher, and FEua levels were lower in men's group. Sex,body mass index (BMI), mean arterial blood pressure (MAP), serum triglycerides (TG), FA and Ccr were independent correlation factors of UA. UA decreased and FEua increased after the patients underwent a combined treatment.Conclusions UA increased initially and then decreased as glucose levels increased from NGT to IGR and T2DM.Compared with NGT and T2DM, IGR subjects had higher SUA levels, which related to its high levels of insulin. Under T2DM, male gender, BMI, MAP, Ccr, TG and FA are independent correlation factors of UA

  2. Deuterium content variation of human blood serum

    International Nuclear Information System (INIS)

    We report, for the first time, the variation of deuterium content of blood serum of the patients with cancer disease. The tumorous cell, because of the higher growth speed , is deuterium consuming. The deuterium content of blood serum of cancerous human is diminished by about 5-7 ppm compared with that of the healthy individual. This effect is in accordance with some previous results. The replacement of tap water with deuterium depleted water in a drinking water of tumorous mice diminished the growth rate of tumors and the slight increase in the deuterium concentration stimulates growth because it is more easy for the cells to elevate the intracellular deuterium concentration up to the threshold level. (authors)

  3. Accuracy of handheld blood glucose meters at high altitude.

    Directory of Open Access Journals (Sweden)

    Pieter de Mol

    Full Text Available BACKGROUND: Due to increasing numbers of people with diabetes taking part in extreme sports (e.g., high-altitude trekking, reliable handheld blood glucose meters (BGMs are necessary. Accurate blood glucose measurement under extreme conditions is paramount for safe recreation at altitude. Prior studies reported bias in blood glucose measurements using different BGMs at high altitude. We hypothesized that glucose-oxidase based BGMs are more influenced by the lower atmospheric oxygen pressure at altitude than glucose dehydrogenase based BGMs. METHODOLOGY/PRINCIPAL FINDINGS: Glucose measurements at simulated altitude of nine BGMs (six glucose dehydrogenase and three glucose oxidase BGMs were compared to glucose measurement on a similar BGM at sea level and to a laboratory glucose reference method. Venous blood samples of four different glucose levels were used. Moreover, two glucose oxidase and two glucose dehydrogenase based BGMs were evaluated at different altitudes on Mount Kilimanjaro. Accuracy criteria were set at a bias 6.5 mmol/L and <1 mmol/L from reference glucose (when <6.5 mmol/L. No significant difference was observed between measurements at simulated altitude and sea level for either glucose oxidase based BGMs or glucose dehydrogenase based BGMs as a group phenomenon. Two GDH based BGMs did not meet set performance criteria. Most BGMs are generally overestimating true glucose concentration at high altitude. CONCLUSION: At simulated high altitude all tested BGMs, including glucose oxidase based BGMs, did not show influence of low atmospheric oxygen pressure. All BGMs, except for two GDH based BGMs, performed within predefined criteria. At true high altitude one GDH based BGM had best precision and accuracy.

  4. Blood glucose fluctuation accelerates renal injury involved to inhibit the AKT signaling pathway in diabetic rats.

    Science.gov (United States)

    Ying, Changjiang; Zhou, Xiaoyan; Chang, Zhenzhen; Ling, Hongwei; Cheng, Xingbo; Li, Wei

    2016-07-01

    Blood glucose fluctuation is associated with diabetic nephropathy. However, the mechanism by which blood glucose fluctuation accelerates renal injury is not fully understood. The aim of the present study was to assess the effects of blood glucose fluctuation on diabetic nephropathy in rats and investigate its underlying mechanism. Diabetes in the rats was induced by a high sugar, high-fat diet, and a single dose of STZ (35 mg/kg)-injected intraperitoneally. Unstable blood sugar models were induced by subcutaneous insulin injection and intravenous glucose injection alternately. Body weight, glycosylated hemoglobin A1c (HbAlc), blood urea nitrogen (BUN), serum creatinine (Scr), and Creatinine clearance (Ccr) were assessed. T-SOD activity and MDA level were measured by assay kit. Change in renal tissue ultrastructure was observed by light microscopy and electron microscopy. Phosphorylated ser/thr protein kinase (p-AKT) (phosphor-Ser473), phosphorylated glycogen synthase kinase-3 beta (p-GSK-3β) (phosphor-Ser9), Bcl-2-associated X protein (BAX), B cell lymphoma/leukemia 2 (BCL-2), and cleaved-cysteinyl aspartate-specific proteinase-3 (caspase-3) levels were detected by immunohistochemistry and Western blot. We observed that BUN and Scr were increased in diabetic rats, and Ccr was decreased. Furthermore, blood glucose fluctuations could exacerbate the Ccr changes. Renal tissue ultrastructure was also seriously injured by glucose variability in diabetic rats. In addition, glucose fluctuation increased the oxidative stress of renal tissue. Moreover, fluctuating blood glucose decreased p-AKT level and BCL-2, and increased p-GSK-3β, BAX, cleaved-caspase-3 levels, and ratio of BAX/BCL-2 in the kidneys of diabetic rats. In conclusion, these results suggest that blood glucose fluctuation accelerated renal injury is due, at least in part to its oxidative stress promoting and inhibiting the AKT signaling pathway in diabetic rats. PMID:26860515

  5. Relationship of serum 25-hydroxyvitamin D and parathyroid hormone levels with blood pressure in 1403 community adults

    Institute of Scientific and Technical Information of China (English)

    王睿

    2014-01-01

    Objective To investigate the association of serum25-hydroxyvitamin D(25OHD),parathyroid hormone(PTH),blood glucose,blood lipid,uric acid,obesity,insulin etc with blood pressure.Methods A total of 1403 residents aged(45.3±14.4)years were enrolled from Guiyang residents during a healthy survey in 2009.The standard questionnaire survey and physical examination were performed in all subjects.The fasting plasma glucose,postprandial 2 h plasma glucose(2h PG),triglyceride,high density lipoprotein-cholesterol,serum

  6. Optical coherence tomography for blood glucose monitoring through signal attenuation

    Science.gov (United States)

    De Pretto, Lucas R.; Yoshimura, Tania M.; Ribeiro, Martha S.; de Freitas, Anderson Z.

    2016-03-01

    Development of non-invasive techniques for glucose monitoring is crucial to improve glucose control and treatment adherence in patients with diabetes. Hereafter, Optical Coherence Tomography (OCT) may offer a good alternative for portable glucometers, since it uses light to probe samples. Changes in the object of interest can alter the intensity of light returning from the sample and, through it, one can estimate the sample's attenuation coefficient (μt) of light. In this work, we aimed to explore the behavior of μt of mouse's blood under increasing glucose concentrations. Different samples were prepared in four glucose concentrations using a mixture of heparinized blood, phosphate buffer saline and glucose. Blood glucose concentrations were measured with a blood glucometer, for reference. We have also prepared other samples diluting the blood in isotonic saline solution to check the effect of a higher multiple-scattering component on the ability of the technique to differentiate glucose levels based on μt. The OCT system used was a commercial Spectral Radar OCT with 930 nm central wavelength and spectral bandwidth (FWHM) of 100 nm. The system proved to be sensitive for all blood glucose concentrations tested, with good correlations with the obtained attenuation coefficients. A linear tendency was observed, with an increase in attenuation with higher values of glucose. Statistical difference was observed between all groups (pcontrol, which eliminates the use of analytes and/or test strips, as in the case with commercially available glucometers.

  7. Towards a Wearable Non-invasive Blood Glucose Monitoring Device

    International Nuclear Information System (INIS)

    Every day, about 150 Million people worldwide face the problem of diabetic metabolic control. Both the hypo- and hyper- glycaemic conditions of patients have fatal consequences and warrant blood glucose monitoring at regular interval. Existing blood glucose monitors can be widely classified into three classes viz., invasive, minimally invasive, and noninvasive. Invasive monitoring requires small volume of blood and are inappropriate for continuous monitoring of blood glucose. Minimally invasive monitors analyze tissue fluid or extract few micro litre of blood only. Also the skin injury is minimal. On the other hand, noninvasive devices are painless and void of any skin injury. We use an indigenously developed polarization sensitive Optical Coherence Tomography to measure the blood glucose levels. Current trends and recent results with the device are discussed.

  8. [Properties of blood serum from reindeer and results of using it in virology and biotechnology].

    Science.gov (United States)

    Gunenkov, V V; Sukharev, O I; Sirota, V F

    2003-01-01

    The blood serum of the reindeer does not virtually contain any antibodies to respiratory and intestinal viruses widely spread among the cattle. The reindeer blood serum is comparable with the bovine serum by the contents of chlorides, hemoglobin, total protein and protein fractions; however, it is different from the latter by a 2.5-fold higher content of glucose and by a higher osmotic pressure. The serum stimulates the mitotic cell activity and cell monolayer production; it also contributes to accumulating the cytopathogenic viruses in cell culture. 5- and 10%-serum, when added to a nutrient medium, ensures the reproduction of bovine leucosis virus and the accumulation of the antigen of this virus in a continuous cell culture that is chronically infected with the virus. Finally, the reindeer serum stabilizes the viability of ovocytes and stimulates the bovine embryo development at transplantation. PMID:14708230

  9. Home blood glucose concentrations in maturity-onset diabetes.

    OpenAIRE

    Paisey, R B; Bradshaw, P; Hartog, M.

    1980-01-01

    Blood glucose concentrations during normal daily activities were measured in 106 patients with maturity-onset diabetes from capillary blood samples collected on to filter paper. Samples were taken before and two hours after main meals, before going to bed, and, in 51 cases, during the night. Fasting and mid-morning values were closely correlated with the mean values over 24 hours irrespective of the type of anti-diabetic treatment being given. Postprandial blood glucose concentrations remaine...

  10. LEVELS OF SELECTED INDICATORS IN THE BLOOD SERUM OF HORSES AND THEIR CORRELATION DEPENDENCY

    OpenAIRE

    Peter Čupka; Marek Halo; Peter Massányi; Anton Kováčik; Robert Stawarz; Formicki Grzegorz

    2013-01-01

    In this study 26 sport horses (14 mares and 12 stallions) were included in the trial. The content of urea, total proteins (TP), glucose (GLU), cholesterol (CHOL), triglycerides (TG), bilirubin (BILI), AST, ALT, GGT and ALP was determined in the blood serum. Levels of monitoring indicators in stallions and mares were balanced. Significant difference (P

  11. Effect of cinnamon extract on blood glucose level and lipid profile in alloxan induced diabetic rats

    International Nuclear Information System (INIS)

    Background: Cinnamon has been shown to potentiate the hypoglycaemic effect of insulin through up regulation of the glucose uptake in cultured adipocytes of rats. This study tried to find out the effect of Cinnamon alone or in combination with Insulin in diabetic albino rats. Methods: Thirty rats were divided into three groups, A and B. Group A were given cinnamon extract 200 mg/Kg body weight daily orally and group B rats were given cinnamon extract 400 mg/Kg body weight daily. After six weeks blood glucose and lipid profile levels were evaluated in all the groups. Results: Group of rats given 200 mg cinnamon extract showed significant decrease of blood glucose concentration but there was slight or no change in the level of lipid parameters including serum cholesterol, triglyceride and lipoproteins (HDL, LDL-chol). On the other hand group of rats given 400 mg extract of cinnamon showed a better but non significant change in level of lipid related parameter while blood glucose level was significantly decreased. Conclusion: The cinnamon at a dose of 400 mg showed same effects on blood glucose level but better effects on lipid profiles especially of serum cholesterol level of group of rats compared to 200 mg of cinnamon extract. Cinnamon may be recommended as hypoglycaemic herb but not as hypolipidaemic herb. (author)

  12. Role of SGK1 in Salt Sensitivity of Blood Pressure and Peripheral Glucose Uptake: Studies in Knockout Mice

    OpenAIRE

    Boini, Krishna Murthy

    2006-01-01

    Excess salt intake increases blood pressure particularly during states of hyperinsulinism and insulin resistance. Insulin is presumably effective through activation of ENaC. Excess salt intake further decreases peripheral glucose uptake thus impairing glucose tolerance. Stimulation of both, the epithelial Na+ channel ENaC and of cellular glucose uptake involves phosphatidylinositide 3-kinase (PI-3K) which signals through protein kinase B (Akt/PKB) and all three members of the serum and glucoc...

  13. High Blood Glucose: What It Means and How To Treat It

    Science.gov (United States)

    ... Blood Glucose: What It Means and How To Treat It What is high blood glucose? People who ... to keep your blood glucose under control, and treat hyperglycemia when it occurs. What are the symptoms ...

  14. SERUM FERRITIN CONCENTRATIONS IN AN IMPAIRED FASTING GLUCOSE POPULATION AND THEIR NORMAL CONTROL GROUP

    Directory of Open Access Journals (Sweden)

    F. Sharifi

    2007-07-01

    Full Text Available Some recent studies have revealed the relationship among excess ferritin, coronary heart disease, and insulin resistance. To assess the association between serum ferritin concentration and impaired fasting glucose, this study was designed. A total of 187 people including 91 impaired fasting glucose (IFG subjects and 96 normal glucose subjects who had been recognized in a large epidemiological study in Zanjan in 2001 were enrolled. The cohorts were well matched for age, sex and BMI. Body mass index and blood pressure of the participants were measured and serum cholesterol, triglyceride and ferritin were evaluated. All the data were analyzed by t-test, x² test and analysis of variance. Serum ferritin was higher in the IFG cohort (85.5 ± 6.6 µg/l vs. 49.4 ± 3.7 µg/l, P = 0.001. A positive correlation was found between fasting plasma glucose and serum ferritin in this study (r: 0.29, P: 0.001. Using multiple regression analysis, we found an association between serum ferritin and BMI (0.06, p: 0.4, blood pressure (0.15, P = 0.01, FPG (0.29, P = 0.001, triglyceride (0.08, P = 0.01 and cholesterol (0.07, P = 0.03. The odd's ratio for the association of IFG in male subjects with the high serum ferritin level was 8.3 (CI 95%:1.211.9, P = 0.01 and for females was 3.06 (C.I 95%: 0.58-15, P: 0.1. Our study, implying that hyperferritinemia occurs before elevation of plasma glucose concentration more than 126 mg/dl. If prospective and interventional studies Confirm an etiologic role of iron overload in the pathogenesis of insulin resistance and type 2 diabetes, reduced dietary iron intake, especially in men with additional risk factors for type 2 diabetes, would appear to be a logical consequence.

  15. Association between serum uric acid and different states of glucose metabolism and glomerular filtration rate

    Institute of Scientific and Technical Information of China (English)

    CAI Xiao-ling; HAN Xue-yao; JI Li-nong

    2010-01-01

    Background Recently, it has been suggested that the serum uric acid (SUA) level decreased in diabetic patients. The aim of this study was to explore the association between SUA level and different state of glucose metabolism and glomerular filtration rate (GFR) reflected by the simplified Modification of Diet in Renal Disease (MDRD) equation and to test the hypothesis that high MDRD is one of the determinants of SUA level.Methods This cross-sectional study included 2373 subjects in Beijing who underwent a 75 g oral glucose tolerance test (OGTT) for screening of diabetes. According to the states of glucose metabolism, they were divided into normal glucose tolerance, impaired glucose regulation and diabetes.Results Multiple stepwise linear regression analysis showed that adjusted by gender, SUA was positively correlated with body mass index (BMI), waist/hippo ratio, systolic blood pressure (SBP) and triglyceride, meanwhile negatively correlated with age, hemoglobin A1c, fasting insulin and MDRD. There was an increasing trend in SUA concentration and a decreasing trend in MDRD when the levels of fasting plasma glucose (FPG) increased from low to high up to the FPG level of 8.0 mmol/L; thereafter, the SUA concentration started to decrease with further increases in FPG levels, and the MDRD started to increase with further increases in FPG levels.Conclusion This study confirmed the previous finding that SUA decreased in diabetes and provided the supporting evidence that the increased MDRD might contribute to the fall of SUA.

  16. Selection of the most informative near infrared spectroscopy wavebands for continuous glucose monitoring in human serum.

    Science.gov (United States)

    Goodarzi, Mohammad; Saeys, Wouter

    2016-01-01

    By controlling the blood glucose levels of diabetics permanent diabetes-related problems such as blindness and loss of limbs can be delayed or even avoided. Therefore, many researchers have aimed at the development of a non-invasive sensor to monitor the blood glucose level continuously. As non-invasive measurements through the skin, the ear lobe or the gums have proven to be either unreliable or impractical, attention has recently shifted to minimally invasive sensors which measure the glucose content in serum or interstitial fluid. Thanks to the development of on-chip spectrometers minimally invasive, implantable devices are coming within reach. However, this technology does not allow to acquire a large number of wavelengths over a broad range. Therefore, the most informative combination of a limited number of variables should be selected. In this study, Interval PLS (iPLS), Variable Importance in Projection (VIP), Uninformative Variable Elimination (UVE), Bootstrap-PLS coefficients, Moving window, CorXyPLS, Interval Random Frog-PLS and combinations of these methods were used in order to address the question whether the short wave band (800-1500 nm), first overtone band (1500-1800 nm), the combination band (2050-2300 nm) or a combination of them is the most informative region for glucose measurements and which wavebands should be measured within these wavelength ranges. The three different data sets employed focus on the determination of (1) glucose in aqueous solutions over the 1-30 mM range in presence of urea and sodium D-lactate, (2) glucose in aqueous solutions over the 2-16 mM range in presence of icodextrin and urea and (3) glucose in human serum samples. The best results for the first, second and third data sets were obtained by selecting 40, 130 and 20 variables resulting in a PLS model with an RMSEP of 0.56, 0.59 and 1.5mM, respectively. It was found that the first overtone band is most informative for aqueous solutions, while for glucose measurement of

  17. Lithium modulates the chronic stress-induced effect on blood glucose level of male rats

    Directory of Open Access Journals (Sweden)

    Popović Nataša

    2010-01-01

    Full Text Available In the present study we examined gross changes in the mass of whole adrenal glands and that of the adrenal cortex, as well as the serum corticosterone and glucose level of mature male Wistar rats subjected to three different treatments: animals subjected to chronic restraint-stress, animals injected with lithium (Li and chronically stressed rats treated with Li. Under all three conditions we observed hypertrophy of whole adrenals, as well as the adrenal cortices. Chronic restraint stress, solely or in combination with Li treatment, significantly elevated the corticosterone level, but did not change the blood glucose level. Animals treated only with Li exhibited an elevated serum corticosterone level and blood glucose level. The aim of our study was to investigate the modulation of the chronic stress-induced effect on the blood glucose level by lithium, as a possible mechanism of avoiding the damage caused by chronic stress. Our results showed that lithium is an agent of choice which may help to reduce stress-elevated corticosterone and replenish exhausted glucose storages in an organism.

  18. Optical coherence tomography for blood glucose monitoring through signal attenuation

    Science.gov (United States)

    De Pretto, Lucas R.; Yoshimura, Tania M.; Ribeiro, Martha S.; de Freitas, Anderson Z.

    2016-03-01

    Development of non-invasive techniques for glucose monitoring is crucial to improve glucose control and treatment adherence in patients with diabetes. Hereafter, Optical Coherence Tomography (OCT) may offer a good alternative for portable glucometers, since it uses light to probe samples. Changes in the object of interest can alter the intensity of light returning from the sample and, through it, one can estimate the sample's attenuation coefficient (μt) of light. In this work, we aimed to explore the behavior of μt of mouse's blood under increasing glucose concentrations. Different samples were prepared in four glucose concentrations using a mixture of heparinized blood, phosphate buffer saline and glucose. Blood glucose concentrations were measured with a blood glucometer, for reference. We have also prepared other samples diluting the blood in isotonic saline solution to check the effect of a higher multiple-scattering component on the ability of the technique to differentiate glucose levels based on μt. The OCT system used was a commercial Spectral Radar OCT with 930 nm central wavelength and spectral bandwidth (FWHM) of 100 nm. The system proved to be sensitive for all blood glucose concentrations tested, with good correlations with the obtained attenuation coefficients. A linear tendency was observed, with an increase in attenuation with higher values of glucose. Statistical difference was observed between all groups (p<0.001). This work opens the possibility towards a non-invasive diagnostic modality using OCT for glycemic control, which eliminates the use of analytes and/or test strips, as in the case with commercially available glucometers.

  19. A data driven nonlinear stochastic model for blood glucose dynamics.

    Science.gov (United States)

    Zhang, Yan; Holt, Tim A; Khovanova, Natalia

    2016-03-01

    The development of adequate mathematical models for blood glucose dynamics may improve early diagnosis and control of diabetes mellitus (DM). We have developed a stochastic nonlinear second order differential equation to describe the response of blood glucose concentration to food intake using continuous glucose monitoring (CGM) data. A variational Bayesian learning scheme was applied to define the number and values of the system's parameters by iterative optimisation of free energy. The model has the minimal order and number of parameters to successfully describe blood glucose dynamics in people with and without DM. The model accounts for the nonlinearity and stochasticity of the underlying glucose-insulin dynamic process. Being data-driven, it takes full advantage of available CGM data and, at the same time, reflects the intrinsic characteristics of the glucose-insulin system without detailed knowledge of the physiological mechanisms. We have shown that the dynamics of some postprandial blood glucose excursions can be described by a reduced (linear) model, previously seen in the literature. A comprehensive analysis demonstrates that deterministic system parameters belong to different ranges for diabetes and controls. Implications for clinical practice are discussed. This is the first study introducing a continuous data-driven nonlinear stochastic model capable of describing both DM and non-DM profiles. PMID:26707373

  20. Prediction methods for blood glucose concentration design, use and evaluation

    CERN Document Server

    Jørgensen, John; Renard, Eric; Re, Luigi

    2016-01-01

    This book tackles the problem of overshoot and undershoot in blood glucose levels caused by delay in the effects of carbohydrate consumption and insulin administration. The ideas presented here will be very important in maintaining the welfare of insulin-dependent diabetics and avoiding the damaging effects of unpredicted swings in blood glucose – accurate prediction enables the implementation of counter-measures. The glucose prediction algorithms described are also a key and critical ingredient of automated insulin delivery systems, the so-called “artificial pancreas”. The authors address the topic of blood-glucose prediction from medical, scientific and technological points of view. Simulation studies are utilized for complementary analysis but the primary focus of this book is on real applications, using clinical data from diabetic subjects. The text details the current state of the art by surveying prediction algorithms, and then moves beyond it with the most recent advances in data-based modeling o...

  1. Temperature influence on non-invasive blood glucose measurement

    Science.gov (United States)

    Zhang, Xiqin; Yeo, Joon Hock

    2009-02-01

    Regular monitoring of blood sugar level is important for the management of diabetes. The Near-Infra-Red (NIR) spectroscopy method is a promising approach and this involves some form of contact with the body skin. It is noted that the skin temperature does fluctuate with the environment and physiological conditions and the temperature has an influence on the glucose measurement. In this paper, in-vitro and in-vivo investigations on the temperature influence on blood glucose measurement were studied. The in-vitro results from FTIR spectrometer show that sample temperature has significant influence on water absorption, which significantly affects the glucose absorption measurement. The in-vivo results show that when skin temperature around the measurement site is taken into consideration, the prediction of blood glucose level greatly improves.

  2. Comparative Analysis of Serum Renal Function, Blood Glucose and Amylase in Different Detection Systems%不同检测系统测定血清肾功、血糖、淀粉酶结果对比分析

    Institute of Scientific and Technical Information of China (English)

    徐新生

    2015-01-01

    目的:探讨不同检测系统测定血清肾功、血糖、淀粉酶结果对比;方法将我院检验科收入的300例患者的血清样品分别采用两种不同的检测系统进行检验,比较两种检测系统血清肾功、血糖及淀粉酶水平;结果不同检测系统测定的肾功、血糖及淀粉酶结果无差异,比较无统计学意义(>0.05);不同检测系统在肌酐、血糖及淀粉酶测定的精密度无差异;结论两种检测系统对肾功、血糖及淀粉酶测定结果具有可比性,测定的精密度较高,能够满足临床使用要求。%Objective To compare the detection results of renal function,blood sugar and amylase based on dif erent systems.Methods 300 serum samples col ected from our Clinical Laboratory were tested by two systems.The levels of renal function,blood sugar and amylase were compared.Results Based on the dif erent detection systems,the levels of renal function,blood sugar and amylase were not dif erent ( <0.05);based on the dif erent systems,the detection precision of Creatinine,blood sugar and amylase was not dif erent.Conclusion The detection results of renal function,blood sugar and amylase based on two systems are comparable and have a higher detection precision,which it conforms to the clinical requirements.

  3. Use of a blood glucose meter for radiochromic film analysis in blood irradiation

    International Nuclear Information System (INIS)

    The use of a diabetic blood glucose meter for radiochromic film dosimetry in blood irradiation using x-ray beams on a medical linear accelerator has been investigated. The glucose meter provides optical density analysis in the visible and infrared region using a reflectance measurement technique. By comparing the 'blood sugar' level output with standard calibration gafchromic films a calibration curve is produced for quantitative analysis. Results show that a reproducible dose to meter output curve can be fitted using a second order polynomial function and that blood irradiation doses in vitro were measured to within 7.9% mean error (as compared to ionization chamber results) using the blood glucose meter. This level of accuracy falls below that measured with a standard densitometer (4.3%); however, results show that the blood glucose meter, which would be available in any haematology department, produces an adequate measure of gafchromic film optical density for blood irradiation dosimetry. (author)

  4. Visual detection of blood glucose based on peroxidase-like activity of WS2 nanosheets.

    Science.gov (United States)

    Lin, Tianran; Zhong, Liangshuang; Song, Zhiping; Guo, Liangqia; Wu, Hanyin; Guo, Qingquan; Chen, Ying; Fu, FengFu; Chen, Guonan

    2014-12-15

    Tungsten disulfide (WS2) nanosheets were discovered to possess intrinsic peroxidase-like activity and catalyze the peroxidase substrate 3,3',5,5'-tetramethylbenzidine (TMB) to produce a color reaction in the presence of H2O2. Based on this finding, a colorimetric method and a portable test kit for the visual detection of blood glucose have been developed by using glucose oxidase (GOx) and WS2 nanosheets-catalyzed reactions. The linear range for glucose was ranged from 5 to 300 μM (R(2)=0.999) with the detection limit of 2.9 μM. The portable test kit was successfully evaluated glucose levels in serum samples from normal persons and diabetes persons by the observable color change from pale yellow to yellow-green, blue-green. PMID:25032681

  5. Effect of Iranian Honey bee (Apis Mellifera Venom on Blood Glucose and Insulin in Diabetic Rats

    Directory of Open Access Journals (Sweden)

    Seyyedeh Mahbubeh Mousavi

    2012-12-01

    Full Text Available Background: Diabetes is an important disease. This disease is a metabolic disorder characterized by hyperglycemia resulting from perturbation in insulin secretion, insulin action or both. Honey bee venom contains a wide range of polypeptide agents. The principle components of bee venom are mellitin and phospholipase A2. These components increase insulin secretion from the β-cells of pancreas. This study was conducted to show the hypoglycemic effect of honey bee venom on alloxan induced diabetic male rats.Methods: Eighteen adult male rats weighting 200±20 g were placed into 3 randomly groups: control, alloxan monohy­drate-induced diabetic rat and treated group that received honey bee venom daily before their nutrition for four months. Forty eight hours after the last injection, blood was collected from their heart, serum was dissented and blood glucose, insulin, triglyceride and total cholesterol were determined.Results: Glucose serum, triglyceride and total cholesterol level in treated group in comparison with diabetic group was significantly decreased (P< 0.01. On the other hand, using bee venom causes increase in insulin serum in com­parison with diabetic group (P< 0.05.Conclusion: Honeybee venom (apitoxin can be used as therapeutic option to lower blood glucose and lipids in dia­betic rats.

  6. The low TSH level in blood serum

    International Nuclear Information System (INIS)

    The low TSH level in blood serum (0.38 - 4,8 mμ/l) and the frequency of cross-reactions dictate the need for a sensitive and specific assay method. The IRMA (immunoradiometric assay) technique meets the requirements for high sensitivity and specificity. In the present study work was done to determine the characteristics of the IRMA-TSH (Netria-Batan) kit which makes use of the ''sandwich'' technique. Samples/standards were reacted with excess I-125 labelled monoclonal antibodies against TSH (Ab*), the free and the bound Ab* fraction were separated using solid phase antibody. The result showed that the IRMA-TSH (Netria-Batan) kit has abord working range (0.2 - 550 mμ/I) and a Low blank (Bo) value (0.8%-0.095%), high precision (with in assay CV less than 10%, and between assay CV less than 15%) high sensitivity (detection limit 0.04 mμ/l), and high accuracy (recovery 94,1%). High specificity of the kit was assured by the use of monoclonal anti-TSH with low cross reaction (0.008% against hCG, 0.7% against LH and 1.7% against FSH (1). (author). 7 figs.; 7 refs

  7. Effect of 'antidiabetis' herbal preparation on serum glucose and fructosamine in NOD mice.

    Science.gov (United States)

    Petlevski, R; Hadzija, M; Slijepcevic, M; Juretic, D

    2001-05-01

    The antihyperglycemic effect of the Antidiabetis herbal preparation ((Myrtilli folium (Vaccinium myrtillus L.), Taraxaci radix (Taraxacum officinale Web.), Cichorii radix (Cichorium intybus L.), Juniperi fructus (Juniperus communis L.), Centaurii herba (Centaurium umbellatum Gilib.), Phaseoli pericarpium (Phaseolus vulgaris), Millefollii herba (Achillea millefolium L.), Morii folium (Morus nigra L.), Valeriane radix (Valleriana officinalis L.), Urticae herba et radix (Urtica dioica L.)), patent No. P-9801091 Zagreb, Croatia was investigated. Two extracts were prepared: ethanol extract (extract 1), and ethanol extract from which ethanol was evaporated on a rotatory evaporator at a temperature of 45 degrees C (extract 2). Extract 1 and extract 2 were administered (in experiment 1) to alloxan-induced non-obese diabetic (NOD) mice in the same dose of 20 mg/kg. Blood glucose was determined before, and 10, 30, 60 and 120 min after the preparation administration. Extract 1 and extract 2 decreased the level of blood glucose by 10 and 20%, respectively, of the initial value (at 0 min, mean = 22.6 +/- 8.3 mmol/l). Serum levels of glucose and fructosamine were determined in NOD mice, NOD mice administered extract 2 in a dose of 20 mg/kg of extract 2, and NOD mice administered acarbose in a dose of 25 mg/100 g chow, in order to verify the hypoglycemic action of extract 2 (in experiment 2). Extract 2 and acarbose were admixed to the chow. The duration of treatment was 7 days. Significantly lower glucose (P < 0.05) and fructosamine (P < 0.001) levels were recorded in extract 2 treated NOD mice as compared with NOD mice. Study results showed extract 2 to significantly decrease the level of glucose and fructosamine in alloxan induced NOD mice. Our future studies will be focused on the search of active principles of the extracts. PMID:11297848

  8. Using statistical quality control techniques to monitor blood glucose levels.

    OpenAIRE

    Oniki, T. A.; Clemmer, T. P.; Arthur, L. K.; Linford, L. H.

    1995-01-01

    Continuous Quality Improvement techniques developed in industry are increasingly being applied to the medical field. Statistical process control charts are a CQI technique aimed at monitoring a process and its variability. At our hospital, statistical quality control charts are being constructed from laboratory blood glucose measurements of patients receiving enteral or parenteral nutrition. The charts will be used to monitor glucose levels, reveal variations, and illustrate the effects of ne...

  9. Radioimmunoassay of serum β2-microglobulin in donor's blood

    International Nuclear Information System (INIS)

    Serum β2-microglobulin (β2-MG) was tested by radioimmunoassay in 149 donors' and 54 healthy volunteers' blood. The results were 203 +- 33.0 nmol/l and 176 +- 26.2 nmol/l, respectively. There was significant difference statistically between them (P2-MG content. In order to increase the quality of donated blood and to keep the health of blood donor, it is suggested that the high content of serum β2-MG is the indicator of too frequent blood donating. The results also showed that the content of β2-MG in donor's blood is not a normal reference value

  10. Inhaled insulin for controlling blood glucose in patients with diabetes

    Directory of Open Access Journals (Sweden)

    Bernard L Silverman

    2008-01-01

    Full Text Available Bernard L Silverman1, Christopher J Barnes2, Barbara N Campaigne3, Douglas B Muchmore31Alkermes, Inc, Cambridge, MA, USA; 2i3 Statprobe, Ann Arbor, MI; 3Eli Lilly and Company, Indianapolis, IN, USAAbstract: Diabetes mellitus is a significant worldwide health problem, with the incidence of type 2 diabetes increasing at alarming rates. Insulin resistance and dysregulated blood glucose control are established risk factors for microvascular complications and cardiovascular disease. Despite the recognition of diabetes as a major health issue and the availability of a growing number of medications designed to counteract its detrimental effects, real and perceived barriers remain that prevent patients from achieving optimal blood glucose control. The development and utilization of inhaled insulin as a novel insulin delivery system may positively influence patient treatment adherence and optimal glycemic control, potentially leading to a reduction in cardiovascular complications in patients with diabetes.Keywords: diabetes, inhaled insulin, cardiovascular disease, blood glucose

  11. Whole blood glucose analysis based on smartphone camera module.

    Science.gov (United States)

    Devadhasan, Jasmine Pramila; Oh, Hyunhee; Choi, Cheol Soo; Kim, Sanghyo

    2015-11-01

    Complementary metal oxide semiconductor (CMOS) image sensors have received great attention for their high efficiency in biological applications. The present work describes a CMOS image sensor-based whole blood glucose monitoring system through a point-of-care (POC) approach. A simple poly-ethylene terephthalate (PET) chip was developed to carry out the enzyme kinetic reaction at various concentrations (110–586 mg∕dL) of mouse blood glucose. In this technique, assay reagent is immobilized onto amine functionalized silica (AFSiO2) nanoparticles as an electrostatic attraction in order to achieve glucose oxidation on the chip. The assay reagent immobilized AFSiO2 nanoparticles develop a semi-transparent reaction platform, which is technically a suitable chip to analyze by a camera module. The oxidized glucose then produces a green color according to the glucose concentration and is analyzed by the camera module as a photon detection technique; the photon number decreases when the glucose concentration increases. The combination of these components, the CMOS image sensor and enzyme immobilized PET film chip, constitute a compact, accurate, inexpensive, precise, digital, highly sensitive, specific, and optical glucose-sensing approach for POC diagnosis. PMID:26524683

  12. Whole blood glucose analysis based on smartphone camera module

    Science.gov (United States)

    Devadhasan, Jasmine Pramila; Oh, Hyunhee; Choi, Cheol Soo; Kim, Sanghyo

    2015-11-01

    Complementary metal oxide semiconductor (CMOS) image sensors have received great attention for their high efficiency in biological applications. The present work describes a CMOS image sensor-based whole blood glucose monitoring system through a point-of-care (POC) approach. A simple poly-ethylene terephthalate (PET) chip was developed to carry out the enzyme kinetic reaction at various concentrations (110-586 mg/dL) of mouse blood glucose. In this technique, assay reagent is immobilized onto amine functionalized silica (AFSiO2) nanoparticles as an electrostatic attraction in order to achieve glucose oxidation on the chip. The assay reagent immobilized AFSiO2 nanoparticles develop a semi-transparent reaction platform, which is technically a suitable chip to analyze by a camera module. The oxidized glucose then produces a green color according to the glucose concentration and is analyzed by the camera module as a photon detection technique; the photon number decreases when the glucose concentration increases. The combination of these components, the CMOS image sensor and enzyme immobilized PET film chip, constitute a compact, accurate, inexpensive, precise, digital, highly sensitive, specific, and optical glucose-sensing approach for POC diagnosis.

  13. High and Low Glycemic Index Mixed Meals and Blood Glucose in Youth with Type 2 Diabetes or Impaired Glucose Tolerance

    OpenAIRE

    Gellar, Lauren; Nansel, Tonja R.

    2009-01-01

    This cross-over pilot study tested blood glucose response to low and high glycemic index meals in 12 obese youth with type 2 diabetes or impaired glucose tolerance. Participants demonstrated significantly lower mean daytime blood glucose and a trend toward lower variability, suggesting a clinically relevant impact of reducing glycemic index.

  14. Effects of MDMA on blood glucose levels and brain glucose metabolism

    Energy Technology Data Exchange (ETDEWEB)

    Soto-Montenegro, M.L.; Vaquero, J.J.; Garcia-Barreno, P.; Desco, M. [Hospital General Universitario Gregorio Maranon, Laboratorio de Imagen, Medicina Experimental, Madrid (Spain); Arango, C. [Hospital General Gregorio Maranon, Departamento de Psiquiatria, Madrid (Spain); Ricaurte, G. [Johns Hopkins University School of Medicine, Department of Neurology, Baltimore, MD (United States)

    2007-06-15

    This study was designed to assess changes in glucose metabolism in rats administered single or repeated doses of MDMA. Two different experiments were performed: (1) A single-dose study with four groups receiving 20 mg/kg, 40 mg/kg, saline or heat, and (2) a repeated-dose study with two groups receiving three doses, at intervals of 2 h, of 5 mg/kg or saline. Rats were imaged using a dedicated small-animal PET scanner 1 h after single-dose administration or 7 days after repeated doses. Glucose metabolism was measured in 12 cerebral regions of interest. Rectal temperature and blood glucose were monitored. Peak body temperature was reached 1 h after MDMA administration. Blood glucose levels decreased significantly after MDMA administration. In the single-dose experiment, brain glucose metabolism showed hyperactivation in cerebellum and hypo-activation in the hippocampus, amygdala and auditory cortex. In the repeated-dose experiment, brain glucose metabolism did not show any significant change at day 7. These results are the first to indicate that MDMA has the potential to produce significant hypoglycaemia. In addition, they show that MDMA alters glucose metabolism in components of the motor, limbic and somatosensory systems acutely but not on a long-term basis. (orig.)

  15. Effects of MDMA on blood glucose levels and brain glucose metabolism

    International Nuclear Information System (INIS)

    This study was designed to assess changes in glucose metabolism in rats administered single or repeated doses of MDMA. Two different experiments were performed: (1) A single-dose study with four groups receiving 20 mg/kg, 40 mg/kg, saline or heat, and (2) a repeated-dose study with two groups receiving three doses, at intervals of 2 h, of 5 mg/kg or saline. Rats were imaged using a dedicated small-animal PET scanner 1 h after single-dose administration or 7 days after repeated doses. Glucose metabolism was measured in 12 cerebral regions of interest. Rectal temperature and blood glucose were monitored. Peak body temperature was reached 1 h after MDMA administration. Blood glucose levels decreased significantly after MDMA administration. In the single-dose experiment, brain glucose metabolism showed hyperactivation in cerebellum and hypo-activation in the hippocampus, amygdala and auditory cortex. In the repeated-dose experiment, brain glucose metabolism did not show any significant change at day 7. These results are the first to indicate that MDMA has the potential to produce significant hypoglycaemia. In addition, they show that MDMA alters glucose metabolism in components of the motor, limbic and somatosensory systems acutely but not on a long-term basis. (orig.)

  16. Exposure-based treatment to control excessive blood glucose monitoring.

    OpenAIRE

    Allen, K D; Evans, J. H.

    2001-01-01

    We investigated an exposure-based procedure for reducing excessive checking of blood glucose by a child with diabetes. In a changing criterion design, an exposure-based procedure was implemented by systematically exposing the child to decreasing amounts of information about blood sugar levels (checking) and thereby increasing exposure to potential hypoglycemia. Access to information was reduced in graduated increments, with the parents setting criteria to levels at which they were willing to ...

  17. Effect of sucrose-containing snacks on blood glucose control.

    Science.gov (United States)

    Wise, J E; Keim, K S; Huisinga, J L; Willmann, P A

    1989-06-01

    To determine whether ingestion of sucrose-containing snacks would affect blood glucose (BG) control, 16 subjects with insulin-dependent diabetes mellitus participated in a 5-day double-blind study at a diabetes camp. Eight subjects in the sucrose group ate sucrose-sweetened snacks twice a day, and 8 subjects in the control group ingested snacks that were sweetened with aspartame. The percentage of total daily calories derived from added sucrose was 7% for the sucrose group and 1% for the control group. Metabolic control was assessed by daily capillary BG measurements obtained before meals and the bedtime snack and by determination of serum fructosamine (F) concentrations on arrival at camp (day 0) and after 5 days on the study protocol (day 5). No significant difference was seen between the groups on day 0 (sucrose group [mean +/- SD]: BG 9.9 +/- 3.6 mM, F 3.54 +/- 0.38 mM; control group: BG 9.1 +/- 2.8 mM, F 3.74 +/- 0.71 mM) or day 5 (sucrose group: BG 8.8 +/- 2.6 mM, F 2.94 +/- 0.32 mM; control group: BG 7.4 +/- 2.8 mM, F 2.92 +/- 0.59 mM). We conclude that ingestion of sucrose, added to snacks in an amount up to 7% of total energy intake, does not adversely affect short-term BG control. PMID:2659302

  18. Hybrid CARS for Non-Invasive Blood Glucose Monitoring

    Science.gov (United States)

    Wang, Xi; Pestov, Dmitry; Zhang, Aihua; Murawski, Robert; Sokolov, Alexei; Welch, George; Laane, Jaan; Scully, Marlan

    2007-10-01

    We develop a spectroscopy technique that combines the advantages of both the frequency-resolved coherent anti-Stokes Raman scattering (CARS) and the time-resolved CARS. We use broadband preparation pulses to get an instantaneous coherent excitation of multiplex molecular vibration levels and subsequent optically shaped time-delayed narrowband probing pulse to detect these vibrations. This technique can suppress the nonresonant background and retrieve the molecular fingerprint signal efficiently and rapidly. We employ this technique to glucose detection, the final goal of which is accurate, non-invasive (i.e. painless) and continuous monitoring of blood glucose concentration in the Diabetes diagnosis to replace the current glucose measurement process, which requires painful fingerpricks and therefore cannot be performed more than a few times a day. We have gotten the CARS spectra of glucose aqueous solution down to 2 mM.

  19. Predictors of Daily Blood Glucose Monitoring in Appalachian Ohio

    Science.gov (United States)

    Raffle, Holly; Ware, Lezlee J.; Ruhil, Anirudh V. S.; Hamel-Lambert, Jane; Denham, Sharon A.

    2012-01-01

    Objective: To determine factors contributing to successful diabetes self-management in Appalachia, as evidenced by daily blood glucose monitoring. Methods: A telephone survey (N = 3841) was conducted to assess health status and health care access. The current investigation is limited to the subset of this sample who report having diabetes (N =…

  20. Dynamical Analysis in the Mathematical Modelling of Human Blood Glucose

    Science.gov (United States)

    Bae, Saebyok; Kang, Byungmin

    2012-01-01

    We want to apply the geometrical method to a dynamical system of human blood glucose. Due to the educational importance of model building, we show a relatively general modelling process using observational facts. Next, two models of some concrete forms are analysed in the phase plane by means of linear stability, phase portrait and vector…

  1. Urinary phthalate metabolite concentrations and blood glucose levels during pregnancy

    Science.gov (United States)

    Robledo, Candace A.; Peck, Jennifer D.; Stoner, Julie; Calafat, Antonia M.; Carabin, Hélène; Cowan, Linda; Goodman, Jean R.

    2016-01-01

    Purpose To examine associations between phthalate metabolite urinary concentrations during early pregnancy and blood glucose levels obtained at the time of screening for gestational diabetes mellitus (GDM). Methods Upon initiation of prenatal care, women with a mean gestational age of 12.8 weeks were recruited for a study of environmental chemical exposures (n = 110) and provided a spot urinary specimen. Blood glucose concentrations (mg/dl) were obtained from the electronic medical record for those patients who did not experience a pregnancy loss and did not transfer care to another facility prior to glucose screening (n = 72). Urinary concentrations of nine phthalate metabolites and creatinine were measured at the US Centers for Disease Control and Prevention. Associations between tertiles of phthalate metabolites concentrations and blood glucose levels were estimated using linear regression. Results Compared to pregnant women in the lowest concentration tertile, women with the highest urinary concentrations (≥3rd tertile) of mono-iso-butyl phthalate (tertile: ≥15.3 μg/l, β = −18.3, 95% CI: −35.4, −1.2) and monobenzyl phthalate (tertile: ≥30.3 μg/l, β = −17.3, 95% CI: −34.1, −0.4) had lower blood glucose levels at the time of GDM screening after adjustment for urinary creatinine and demographic covariates. Conclusion Because maternal glucose levels increase during pregnancy to provide adequate nutrition for fetal growth and development, these findings may have implications for fetal health. However, given the limitations of our study, findings should be interpreted cautiously. PMID:25726127

  2. Fluorescent blood glucose monitor by hemin-functionalized graphene quantum dots based sensing system

    Energy Technology Data Exchange (ETDEWEB)

    He, Yuezhen; Wang, Xiaoxun; Sun, Jian; Jiao, Shoufeng; Chen, Hongqi; Gao, Feng; Wang, Lun, E-mail: wanglun@mail.ahnu.edu.cn

    2014-01-31

    Graphical abstract: -- Highlights: •Hemin is assembled onto the surfaces of graphene quantum dots (GQDs). •With the aid of hemin, H{sub 2}O{sub 2} could quench the FL signal of GQDs obviously. •Based on this effect, a fluorescent platform is proposed for the sensing of glucose. •The proposed method provides a new pathway to explore practical application of GQDs. -- Abstract: In the present work, a highly sensitive and specific fluorescent biosensor for blood glucose monitoring is developed based on hemin-functionalized graphene quantum dots (GQDs) and glucose oxidase (GOx) system. The GQDs which are simply prepared by pyrolyzing citric acid exhibit strong fluorescence and good water-solubility. Due to the noncovalent assembly between hemin and GQDs, the addition of hemin can make hydrogen peroxide (H{sub 2}O{sub 2}) to destroy the passivated surface of GQDs, leading to significant fluorescence quenching of GQDs. Based on this effect, a novel fluorescent platform is proposed for the sensing of glucose. Under the optimized conditions, the linear range of glucose is from 9 to 300 μM, and the limit of detection is 0.1 μM. As unique properties of GQDs, the proposed biosensor is green, simple, cost-efficient, and it is successfully applied to the determination of glucose in human serum. In addition, the proposed method provides a new pathway to further design the biosensors based on the assembly of GQDs with hemin for detection of biomolecules.

  3. Effect of Cucurbita ficifolia and Probiotic Yogurt Consumption on Blood Glucose, Lipid Profile, and Inflammatory Marker in Type 2 Diabetes

    OpenAIRE

    Azade Bayat; Fatemeh Azizi-Soleiman; Motahar Heidari-Beni; Awat Feizi; Bijan Iraj; Reza Ghiasvand; Gholamreza Askari

    2016-01-01

    Background: Control of blood sugar, hypertension, and dyslipidemia are key factors in diabetes management. Cucurbita ficifolia (pumpkin) is a vegetable which has been used traditionally as a remedy for diabetes in Iran. In addition, consumption of probiotics may have beneficial effects on people with Type 2 diabetes. The aim of this study was an investigation of the effects of C. ficifolia and probiotic yogurt consumption alone or at the same time on blood glucose and serum lipids in diabetic...

  4. Effect of n-3 fatty acid supplementation on blood glucose, lipid profile and cytokines in humans: A pilot study

    OpenAIRE

    Raghu, B.; Venkatesan, P

    2008-01-01

    This study was undertaken to evaluate the effects of n-3 fatty acid supplementation on blood glucose, lipid profile and cytokines in humans. Twenty adult healthy subjects were supplemented with 1g/day fish oil concentrate capsules for 2 weeks. Fasting blood samples were taken at baseline and again after 2 week intervention. Fish oil supplementation significantly lowered fasting serum concentrations of total cholesterol, triacylglycerol, very low density lipoprotein and low density lipoprotein...

  5. EFFECT OF ADIPECTOMY ON BLOOD GLUCOSE, SERUM LEPTIN AND ADIPONECTIN IN RATS WITH ALIMENTARY OBESITY%脂肪切除术对营养性肥胖大鼠血糖、瘦素及脂联素的影响

    Institute of Scientific and Technical Information of China (English)

    杨柳; 刘肃; 匡瑞霞; 陈璐

    2012-01-01

    Objective To study the influence of adipectomy on blood glucose, leptin and adiponectin in rats with alimentary obesity. Methods An obese model was created in male rats by feeding with high fat diet, which were then divided into operartion group and control group. The rats in operation group underwent resection of fat surrounding epididymis and kidney, and those in the control group, seperation of fat only was done. The differences of the levels of blood glucose, leptin and adiponectin were measured and compared between the two groups, pre- and postoperatively. Results The concentration of all the above items was significantly different on day 15 after surgery between the two groups, except leptin (t = 4. 25 -13. 34, P<0. 01). Conclusion Resection of a great quantity of fat can lower the blood sugar level in rats with alimentary obesity, raise serum adiponectin, and reduce serum leptin within a short period of time.%目的 研究脂肪切除对营养性肥胖大鼠血糖、瘦素及脂联素的影响.方法 以高脂饮食制备成年雄性肥胖大鼠模型,随机分为手术组与对照组,手术组大鼠实施睾丸旁、肾周脂肪切除术,对照组行阴性对照手术,仅分离双侧睾丸旁、肾周脂肪,术后2、15 d测量并比较两组手术前后空腹血糖、血清瘦素及脂联素的差值.结果 手术组大鼠除术后15d血清瘦素差值与对照组比较差异无显著性外,术后2、15 d其余各项指标比较差异均有显著性(t=4.25~13.34,P<0.01).结论 手术切除大量脂肪可以降低营养性肥胖大鼠的血糖水平,提高脂联素水平,并在短期内降低血清瘦素水平.

  6. Depressed serum erythropoietin in pregnant women with elevated blood lead.

    Science.gov (United States)

    Graziano, J H; Slavkovic, V; Factor-Litvak, P; Popovac, D; Ahmedi, X; Mehmeti, A

    1991-01-01

    During the course of a prospective study of lead exposure and pregnancy outcome in 1,502 women, we tested the hypothesis that environmental lead exposure is associated with depressed serum erythropoietin concentration. At mid-pregnancy and at delivery, blood samples were stratified by hemoglobin concentration; within each hemoglobin stratum, sera of women with the lowest and highest whole blood lead concentrations were selected for serum erythropoietin analysis. Analysis of variance revealed that women with higher blood lead levels had inappropriately low serum erythropoietin at both mid-pregnancy and at delivery. Thus, depressed serum erythropoietin appears to indicate lead nephrotoxicity, and it may also be responsible for the anemia associated with lead poisoning. PMID:1772259

  7. Electroacupuncture for control of blood glucose in diabetes: literature review.

    Science.gov (United States)

    Peplow, Philip V; Baxter, G David

    2012-02-01

    Electrical stimulation at acupuncture points (acupoints) has been investigated for its utility in lowering blood glucose in hyperglycemic humans and animal models. Only two studies were found using electroacupuncture in human subjects, and in both of these, the participants were normal (nondiabetic) and electrical stimulation was carried out at several acupoints. It had a hypoglycemic effect in obese women with calorific restriction diet using electrical stimulation of 2 Hz for 30 minutes/day for 20 days, but no change occurred in blood glucose of fasted patients in the other study using 1 Hz for 15 minutes. Fourteen animal studies were found, of which, 11 were performed in diabetic and normal rats. A hypoglycemic effect was observed in fasted type 1 diabetic rats using the Zusanli (ST36) leg acupoint with electrical stimulation of 15 Hz for 30 minutes and 60 minutes. In fasted type 2 diabetic rats, blood glucose was lowered using the Zusanli acupoint with electrical stimulation parameters of 15 Hz and 10 mA for 30 minutes. Also, using the Zhongwan (CV12) abdomen acupoint with electrical stimulation parameters of 15 Hz and 10 mA for 90 minutes had a hypoglycemic effect in fasted type 2 diabetic rats. In fasted normal rats, electrical stimulation of 2 Hz or 15 Hz for 30 minutes at the Zusanli or Zhongwan acupoint caused a decrease in blood glucose. Future studies are required in fasted diabetic rats to determine the effect of electroacupuncture on blood levels of insulin, lipids, fatty acids and β-endorphin, and blood flow and nerve conduction velocity. Studies with fasted normal and diabetic human subjects treated by electroacupuncture are warranted using data from animal experiments to inform such studies. PMID:22309901

  8. Influence of Artificial Sweetener on Human Blood Glucose Concentration

    Directory of Open Access Journals (Sweden)

    Ilse Skokan

    2007-01-01

    Full Text Available Artificial sweeteners, such as saccharin or cyclamic acid are synthetically manufactured sweetenings. Known for their low energetic value they serve especially diabetic and adipose patients as sugar substitutes. It has been hypothesized that the substitution of sugar with artificial sweeteners may induce a decrease of the blood glucose. The aim of this study was to determine the reliability of this hypothesis by comparing the influence of regular table sugar and artificial sweeteners on the blood glucose concentration. In this pilot-study 16 patients were included suffering from adiposity, pre-diabetes and hypertension. In the sense of a cross-over design, three test trials were performed at intervals of several weeks. Each trial was followed by a test free interval. Within one test trial each patient consumed 150 ml test solution (water that contained either 6 g of table sugar (“Kandisin” with sweetener free serving as control group. Tests were performed within 1 hr after lunch to ensure conditions comparable to patients having a desert. Every participant had to determine their blood glucose concentration immediately before and 5, 15, 30 and 60 minutes after the intake of the test solution. For statistics an analysis of variance was performed. The data showed no significant changes in the blood glucose concentration. Neither the application of sugar (F4;60 = 1.645; p = .175 nor the consumption of an artificial sweetener (F2.068;31.023 = 1.551; p > .05 caused significant fluctuations in the blood sugar levels. Over a time frame of 60 minutes in the control group a significant decrease of the blood sugar concentration was found (F2.457;36.849 = 4.005; p = .020 as a physiological reaction during lunch digestion.

  9. Near-infrared spectral methods for noninvasively measuring blood glucose

    Science.gov (United States)

    Fei, Sun; Kong, Deyi; Mei, Tao; Tao, Yongchun

    2004-05-01

    Determination of blood glucose concentrations in diabetic patients is a frequently occurring procedure and an important tool for diabetes management. Use of noninvasive detection techniques can relieve patients from the pain of frequent finger pokes and avoid the infection of disease via blood. This thesis discusses current research and analyzes the advantages and shortages of different measurement methods, including: optical methods (Transmission, Polarimetry and scattering), then, we give emphasis to analyze the technology of near-infrared (NIR) spectra. NIR spectral range 700 nm ~2300 nm was used because of its good transparency for biological tissue and presence of glucose absorption band. In this work, we present an outline of noninvasive blood glucose measurement. A near-infrared light beam is passed through the finger, and the spectral components of the emergent beam are measured using spectroscopic techniques. The device includes light sources having the wavelengths of 600 nm - 1800 nm to illuminate the tissue. Receptors associated with the light sources for receiving light and generating a transmission signal representing the light transmitted are also provided. Once a transmission signal is received by receptors, and the high and low values from each of the signals are stored in the device. The averaged values are then analyzed to determine the glucose concentration, which is displayed on the device.

  10. Noninvasive biosensor and wireless interrogating system for glucose in blood

    Science.gov (United States)

    Varadan, Vijay K.; Whitchurch, Ashwin K.; Sarukesi, K.

    2003-07-01

    Hypoglycemia-abnormal decrease in blood sugar-is a major obstacle in the management of diabetes and prevention of long-term complications, and it may impose serious effects on the brain, including impairment of memory and other cognitive functions. This paper presents the development of a non-invasive sensor with miniaturized telemetry device in a wrist-watch for monitoring glucose concentration in blood. The sensor concept is based on optical chirality of glucose level in the interstitial fluid. The wrist watch consists of a laser power source of the wavelength compatible with the glucose. A nanofilm with specific chirality is placed at the bottom of the watch. The light then passes through the film and illuminates a small area on the skin. It has been documented that there is certain concentration of sugar level is taken by the intertitial fluid from the blood stream and deposit a portion of it at the dead skin. The wrist-watch when in contact with the outer skin of the human will thus monitor the glucose concentration. A wireless monitoring system in the watch then downloads the data from the watch to a Palm or a laptop computer.

  11. The Relationship between Serum Ferritin and Insulin Resistance in Different Glucose Metabolism in Nonobese Han Adults

    OpenAIRE

    Bo-wei Liu; Xu-min Xuan; Jun-ru Liu; Fang-ning Li; Fu-Zai Yin

    2015-01-01

    The exact mechanism through which elevated serum ferritin promotes the development of type 2 diabetes is unknown. This study showed that ferritin concentration in impaired glucose regulation and newly diagnosed diabetes mellitus subjects of nonobesity already significantly increased when compared with normal glucose tolerant subjects of nonobesity. Elevated serum ferritin levels are associated with insulin resistance and may be not associated with the decline of insulin beta cells in differen...

  12. Facilitated transport of glucose from blood to brain in man and the effect of moderate hypoglycaemia on cerebral glucose utilization

    International Nuclear Information System (INIS)

    The effect of steady-state moderate hypoglycaemia on human brain homeostasis has been studied with positron emission tomography using D-glucose 11C(ul) as tracer. To rule out any effects of insulin, the plasma insulin concentration was maintained at the same level under normo- and hypoglycaemic conditions. Reduction of blood glucose by 55% increased the glucose clearance through the blood-brain barrier by 50% and reduced brain glucose consumption by 40%. Blood flow was not affected. The results are consistent with facilitated transport of glucose from blood to brain in humans. The maximal transport rate of glucose from blood to brain was found to be 62±19 (mean±SEM) μmol hg-1 min-1, and the half-saturation constant was found to be 4.1±3.2 mM. (orig.)

  13. Pre-exercise glucose ingestion at different time periods and blood glucose concentration during exercise.

    Science.gov (United States)

    Tokmakidis, S P; Volaklis, K A

    2000-08-01

    The purpose of this study was to investigate the effects of glucose ingestion (GI) at different time periods prior to exercise on blood glucose (BG) levels during prolonged treadmill running. Eight subjects (X+/-SD), age 20+/-0.5yr, bodymass 70.7+/-4.1 kg, height 177+/-4 cm, VO2max 52.8+/-7.8 ml x kg(-1) x min(-1) who underwent different experimental conditions ingested a glucose solution (1 g/kg at 350 ml) 30 min (gl-30), 60 min (gl-60), 90 min (gl-90), and a placebo one 60 min (pl-60) prior to exercise in a counterbalanced design. Afterwards they ran at 65% of VO2max for 1 hour and then at 75 % of VO2max till exhaustion. Fingertip blood samples (10 microl) were drawn every 15 min before and during exercise for the determination of BG levels. Oxygen uptake (VO2), heart rate (HR), and blood lactate (La) were also measured every 15 min during exercise. Peak BG values were reached within 30 min after GI but were different (p affect the duration of treadmill running. The La levels were higher (p affecting the duration of running performance at 75% VO2max. Thus, in order to maintain normoglycaemic levels, pre-exercise glucose supplementation should be given 30 min before the onset of exercise. PMID:10961522

  14. Association of serum uric acid with blood urea and serum creatinine

    International Nuclear Information System (INIS)

    Background: Hyperuricemia can cause serious health problems including renal insufficiency. Hyperuricemia is associated with many diseases including Hypertension, Diabetes Mellitus, Hypertriglyceridemia and Obesity. Objective of the present study was to study the Association of Serum Uric Acid with Blood Urea and Serum Creatinine. Methods: Eighty subjects, aged above 40, having blood urea more than 40 mg/dl and serum Creatinine more than 1.3 mg/dl were selected. 52.5 % subjects were male. Eighty subjects were selected as control group matching the age and sex with study group with normal blood urea and serum Creatinine. Results: Serum Uric Acid was found to be raised in 33 patients. Mean Serum Uric Acid value was 6.98+-2.021 in males (p<0.05) and 5.054+-2.324 in females (p<0.05). Conclusion: Serum Uric Acid is raised in patients with impaired renal function (p<0.05). Levels of increased Serum Uric Acid were not significantly associated with the cause of renal disease. (author)

  15. Inhaled insulin for controlling blood glucose in patients with diabetes

    OpenAIRE

    Silverman, Bernard L; Barnes, Christopher J.; Campaigne, Barbara N.; Muchmore, Douglas B.

    2007-01-01

    Diabetes mellitus is a significant worldwide health problem, with the incidence of type 2 diabetes increasing at alarming rates. Insulin resistance and dysregulated blood glucose control are established risk factors for microvascular complications and cardiovascular disease. Despite the recognition of diabetes as a major health issue and the availability of a growing number of medications designed to counteract its detrimental effects, real and perceived barriers remain that prevent patients ...

  16. Influence of Artificial Sweetener on Human Blood Glucose Concentration

    OpenAIRE

    Ilse Skokan; P. Christian Endler; Beatrix Wulkersdorfer; Dieter Magometschnigg; Heinz Spranger

    2007-01-01

    Artificial sweeteners, such as saccharin or cyclamic acid are synthetically manufactured sweetenings. Known for their low energetic value they serve especially diabetic and adipose patients as sugar substitutes. It has been hypothesized that the substitution of sugar with artificial sweeteners may induce a decrease of the blood glucose. The aim of this study was to determine the reliability of this hypothesis by comparing the influence of regular table sugar and artificial sweeteners on the b...

  17. Perioperative control of blood glucose level in cardiac surgery.

    OpenAIRE

    Minakata, Kenji; Sakata, Ryuzo

    2013-01-01

    It is well recognized that poor perioperative blood glucose (BG) control can increase the risk of infection, cardiovascular accidents, and even death in patients undergoing cardiac surgery. Since it has been reported that tight BG control (80-110 mg/dL) yields better outcomes in critically ill patients, it became a standard of care to control BG using intravenous insulin infusion in ICU. However, it has been debated in terms of the optimal target range whether a strict control with intensive ...

  18. TRANSFERRIN: VARIATIONS IN BLOOD SERUM OF RED HOWLER MONKEYS.

    Science.gov (United States)

    SCHOEN, M A; ARENDS, T

    1964-11-01

    Blood serum samples from 33 red howler monkeys (Alouatta seniculus) were examined. Three different phenotypes were found and denominated A, B, and C. Four serums could not be classified because their transferrin apparently did not bind iron-59, possibly owing to saturation. A difference was observed in the electrophoretic migration and pattern of the transferrins in these monkeys compared with those of other primates. PMID:14197564

  19. Effect of whey on blood glucose and insulin responses to composite breakfast and lunch meals in type 2 diabetic subjects

    DEFF Research Database (Denmark)

    Frid, Anders H; Nilsson, Mikael; Holst, Jens Juul;

    2005-01-01

    breakfast and lunch meals were supplemented with whey on one day; whey was exchanged for lean ham and lactose on another day. Venous blood samples were drawn before and during 4 h after breakfast and 3 h after lunch for the measurement of blood glucose, serum insulin, glucose-dependent insulinotropic......BACKGROUND: Whey proteins have insulinotropic effects and reduce the postprandial glycemia in healthy subjects. The mechanism is not known, but insulinogenic amino acids and the incretin hormones seem to be involved. OBJECTIVE: The aim was to evaluate whether supplementation of meals with a high...... glycemic index (GI) with whey proteins may increase insulin secretion and improve blood glucose control in type 2 diabetic subjects. DESIGN: Fourteen diet-treated subjects with type 2 diabetes were served a high-GI breakfast (white bread) and subsequent high-GI lunch (mashed potatoes with meatballs). The...

  20. Effect of Blood Glucose Fluctuation on Some Trace Elements and Aldosterone Hormone among Type II Diabetic Patients with Metabolic Syndrome

    International Nuclear Information System (INIS)

    There is accumulating evidence determine that the metabolism of some trace elements is altered in diabetes mellitus (DM) type II. The current study aimed to evaluate the effect of serum blood glucose fluctuation during (Random, Fasting and Postprandial 2 hours state) on some trace elements such as Cadmium (Cd), Chromium (Cr), Manganese (Mn), Magnesium (Mg), Zinc (Zn), Copper (Cu), Sodium (Na), Potassium (K), and Aldosterone hormone in type II Diabetic patients associated with metabolic syndrome in comparison with healthy volunteers. The International Diabetes Federation (IFD) consensus the diagnosis of metabolic syndrome according to central obesity, lipid profile, blood glucose level and blood pressure. A significant change was observed in trace elements level (Cd, Cr, Mg, Mn, Zn, Cu, Na, and K) and Aldosterone hormone as a result of glucose fluctuation among type II diabetic patients.

  1. Diurnal Variations in Serum Glucose, Insulin and C-Peptide of Normal Korean Adults

    International Nuclear Information System (INIS)

    It is already well known that many factors are involved in maintaining normal blood glucose level. The amount and components of meal are also thought to be some of the factors which affect the blood glucose and insulin levels. It is reported that as for Koreans sugar takes up over 75% out of 2,098 kcal, the average daily calorie intake per adult. It implies that Koreans take a high-sugar diet compared with Westerners who take 40-50% of sugar out of their total average daily calorie. For the purpose of studying diurnal variations in serum glucose, insulin and C-peptide of normal Koreans adults based on ordinary Korean diet, we selected 13 normal Korean male adults and divided them into two groups, Group I (7 persons) and Group II (6 persons). We put Group I on 3,100 kcal and 75% sugar diet, and Group II on 2,100 kcal and 69% sugar diet per day for over 4 days. Serum glucose, insulin and C-peptide were checked every 30 minutes or every hour throughout 2 hour. Results are as follows: 1. As for serum glucose level, in the preprandial fasting state in the morning, mean±S.D. of Group I was 91.1±3.2 mg%, while that of Group II is 82.5±4.4 mg%. Both groups showed peaks of increased glucose level t postprandial 1 hour after each meal. The peak returned to the level shown during the fasting state at postprandial 1 hour after breakfast while the relatively high glucose levels were maintained respectively even for 2 or 3 hours after lunch and dinner. 2. As for serum insults level, Group I showed mean±S.D. of 14.7±3.0 μU/ml while Group II shows that of 7.0±2.6 μU/ml in the fasting state. Group I particularly showed the largest peak from preprandial a half or one and half an hour to postprandial one hour of lunch, and made relatively small peaks (47.7±10.8 μU/ml) at postprandial 1 hour after breakfast and dinner. No such large peak was marked in Group II, though it showed relatively similar patterns of peak after each meal. 3. As for C-peptide, in the fasting state

  2. The Effect of Methanolic Extract of Otostegia persica on Serum Glucose Level and Renal Function Indicators in Streptozotocin Induced Diabetic Rats

    Directory of Open Access Journals (Sweden)

    Mahdiye Hedayati

    2012-05-01

    Full Text Available Background: Regarding the antioxidant property of Otostegia persica extract and the role of antioxidants in Diabetes mellitus treatment, in this study the effect of extract on serum glucose level and renal function indicators was determined in diabetic male rats. Materials and Methods: Diabetes mellitus (type I was inducted in male rats using intraperitoneal injection of streptozotocin (STZ (65 mg/kg. To determine blood glucose, urea, and creatinine serum levels; fasting blood samples were collected twice (before STZ injection and 5 days later. The rats with their serum glucose level exceeding 250 mg/dl were considered diabetic and divided into 10 groups separately received Otostegia persica alcoholic extract (100, 200, and 300 mg/kg/day doses, glibenclamide with 600 µg/kg dose and 0.5 ml distilled water for 3 and 6 days using gavage. After 3 and 6 days, blood samples were collected again and glucose, urea, and creatinine serum levels were assessed using spectrophotometry technique by respective kits.Results: Treating diabetic rats by Otostegia persica extract (100, 200, and 300 mg/kg/day doses for 6 days results in a significant decrease of glucose and creatinine, yet an increase of serum urea with 200 mg/kg dose. Also, administration of the extract for 3 days (300 mg/kg reduced glucose, and (in various doses urea and creatinine serum levels. Conclusion: Otostegia persica extract has hypoglycemic effect and administering it in diabetes mellitus not only had no undesirable renal side effects, but also improved renal function to some extent.

  3. Evaluation of the appropriateness of using glucometers for measuring the blood glucose levels in mice.

    Science.gov (United States)

    Togashi, Yu; Shirakawa, Jun; Okuyama, Tomoko; Yamazaki, Shunsuke; Kyohara, Mayu; Miyazawa, Ayumi; Suzuki, Takafumi; Hamada, Mari; Terauchi, Yasuo

    2016-01-01

    Glucometers are also widely used in diabetes research conducted using animal models. However, the appropriateness of measuring blood glucose levels using glucometers in animal models remains unclear. In this study, we evaluated the consistency between the blood glucose levels measured by 11 models of glucometers and plasma glucose levels measured by a laboratory biochemical test in blood samples collected by retro-orbital sinus puncture or tail-tip amputation. In both blood samples obtained by retro-orbital sinus puncture and those obtained by tail-tip amputation, 10 of the 11 models of glucometers yielded higher glucose values, while 1 yielded lower glucose values, than the plasma glucose values yielded by the laboratory test, the differences being in direct proportion to the plasma glucose values. Most glucometers recorded higher blood glucose levels after glucose loading and lower blood glucose levels after insulin loading in retro-orbital sinus blood as compared to tail vein blood. Our data suggest that the blood glucose levels measured by glucometers in mice tended to be higher than the plasma glucose levels yielded by the biochemical test under the hyperglycemic state, and that differences in the measured levels were observed according to the blood collection method depending on the glycemia status. PMID:27151424

  4. Splanchnic blood flow and hepatic glucose production in exercising humans

    DEFF Research Database (Denmark)

    Bergeron, R; Kjaer, M; Simonsen, L;

    2001-01-01

    The study examined the implication of the renin-angiotensin system (RAS) in regulation of splanchnic blood flow and glucose production in exercising humans. Subjects cycled for 40 min at 50% maximal O(2) consumption (VO(2 max)) followed by 30 min at 70% VO(2 max) either with [angiotensin-converti......The study examined the implication of the renin-angiotensin system (RAS) in regulation of splanchnic blood flow and glucose production in exercising humans. Subjects cycled for 40 min at 50% maximal O(2) consumption (VO(2 max)) followed by 30 min at 70% VO(2 max) either with [angiotensin......-converting enzyme (ACE) blockade] or without (control) administration of the ACE inhibitor enalapril (10 mg iv). Splanchnic blood flow was estimated by indocyanine green, and splanchnic substrate exchange was determined by the arteriohepatic venous difference. Exercise led to an approximately 20-fold increase (P ...-blockade group vs. the control group, hormones, metabolites, VO(2), and RER followed the same pattern of changes in ACE-blockade and control groups during exercise. Splanchnic blood flow (at rest: 1.67 +/- 0.12, ACE blockade; 1.59 +/- 0.18 l/min, control) decreased during moderate exercise (0.78 +/- 0.07, ACE...

  5. Comparison and Correlation of Glucose Levels in Serum and Saliva of Both Diabetic and Non-diabetic Patients

    OpenAIRE

    Patel, Bhumika J.; Dave, Bela; Dave, Dilip; Karmakar, Payel; Shah, Mona; Sarvaiya, Bhumi

    2015-01-01

    Background: To detect and compare salivary glucose with plasma glucose level and postprandial blood sugar (PPBS) and fasting blood sugar (FBS) in diabetic and non-diabetic subjects. Materials and Methods: A total of 100 patients were participated in this study. They were divided into two groups, each group consist of 50 patients. Un-stimulated saliva and blood were collected and investigated for glucose levels. Results: FBS, PPBS, plasma glucose levels and salivary glucose levels were higher ...

  6. Reagents for Lithium Electrodes and Sensors for Blood Serum Analysis

    Directory of Open Access Journals (Sweden)

    Gary D. Christian

    2002-10-01

    Full Text Available The measurement of lithium in blood serum requires high selectivity since the blood contains about 140 mM sodium compared to the 0.5-1.5 mM lithium level in manic depressive patients under treatment with lithium salts. This review traces the development of optical and potentiometric methods for the selective measurement of lithium in the presence of sodium. Selectivities of over 1,000:1 are achievable with properly designed ionophores.

  7. Capillary Bedside Blood Glucose Measurement in Neonates: Missing a Diagnosis of Galactosemia

    OpenAIRE

    Özbek, Mehmet Nuri; Öcal, Murat; Tanrıverdi, Sibel; Baysal, Birsen; Deniz, Ahmet; Öncel, Kahraman; Demirbilek, Hüseyin

    2015-01-01

    A number of factors may lead to inaccuracy in measurement of capillary blood glucose with a glucometer. Measurement of other carbohydrate molecules such as galactose and fructose along with glucose can potentially be a cause of error. We report a newborn patient who was referred to our hospital with conjugated bilirubinemia, hepatomegaly and high capillary blood glucose levels measured with a glucometer. Simultaneous biochemical measurements revealed normal blood glucose levels. Further inves...

  8. Assessment of Self-Monitored Blood Glucose Results Using a Reflectance Meter with Memory and Microcomputer

    OpenAIRE

    Kuykendall, V.G.; Michaels, D W; Hartmann, K.G.

    1985-01-01

    A microcomputer software package for diabetes patient care utilizing self-monitoring of blood glucose (SMBG) has been developed. The software facilitates the collection, storage, analysis, and presentation of blood glucose/time information. Data entry is accomplished automatically via interface to hand held blood glucose reflectance instruments which retain up to 339 glucose/time results in internal RAM. The times of other significant clinical events may also be stored in the meter and upload...

  9. Effects of exposure to electromagnetic field radiation (EMFR generated by activated mobile phones on fasting blood glucose

    Directory of Open Access Journals (Sweden)

    Sultan Ayoub Meo

    2013-04-01

    Full Text Available Objective: Extensive use of mobile phones has been accompanied by a common public debate about possible adverse effects on human health. No study has been published so far to establish any association between the fastest growing innovation of mobile phone and fasting blood glucose. The aim was to determine the effects of exposure to electromagnetic field radiation generated by mobile phones on fasting blood glucose in Wistar Albino rats. Materials and Methods: 40 Male Albino rats (Wistar Strain were divided into 5 equally numerous groups. Group A served as the control one, group B received mobile phone radiation for less than 15 min/day, group C: 15-30 min/day, group D: 31-45 min/day, and group E: 46-60 min/day for a total period of 3 months. Fasting blood glucose was determined by using Spectrophotometer and serum insulin by Enzyme-linked Immunosorbent Assay (ELISA. The Homeostatic Model (HOMA-B was applied for the assessment of β-cell function and (HOMA-IR for resistance to insulin. Results: Wister Albino rats exposed to mobile phone radiation for longer than 15 min a day for a total period of 3 months had significantly higher fasting blood glucose (p < 0.015 and serum insulin (p < 0.01 compared to the control group. HOMA-IR for insulin resistance was significantly increased (p < 0.003 in the groups that were exposed for 15-30 and 46-60 min/day compared to the control rats. Conclusion: The results of the present study show an association between long-term exposure to activated mobile phones and increase in fasting blood glucose and serum insulin in Albino rats.

  10. Development of a fluorescent method for simultaneous measurement of glucose concentrations in interstitial fluid and blood

    International Nuclear Information System (INIS)

    Continuous blood glucose monitoring is of great clinical significance to patients with diabetes. One of the effective methods to monitor blood glucose is to measure glucose concentrations of interstitial fluid (ISF). However, a time-delay problem exists between ISF and blood glucose concentrations, which results in difficulty in indicating real-time blood glucose concentrations. Therefore, we developed a fluorescent method to verify the accuracy and reliability of simultaneous ISF and blood glucose measurement, especially incorporating it into research on the delay relationship between blood and ISF glucose changes. This method is based on a competitive reaction among borate polymer, alizarin and glucose. When glucose molecules combine with borate polymers in alizarin–borate polymer competitively, changes in fluorescence intensity demonstrate changes in glucose concentrations. By applying the measured results to the blood and ISF glucose delay relationship, we were able to calculate the time delay as an average of 2.16 ± 2.05 min for ISF glucose changes with reference to blood glucose concentrations. (paper)

  11. Serum Levels of Osteocalcin and Insulin Resistance in Patients with Impaired Glucose Tolerance or New-Onset Diabetes Mellitus After Liver Transplantation.

    Science.gov (United States)

    Martínez-Díaz-Guerra, G; Guadalix, S; Aramendi, M; Librizzi, S; García, E; Jiménez, C; Hawkins, F

    2016-05-01

    Liver transplantation (LT) patients are at high risk of developing new-onset diabetes after transplantation (NODAT). Osteocalcin has been proposed as a mediator between bone tissue and glucose metabolism, but its role in the pathogenesis of diabetes is not defined yet. Our objective was to assess the relationship between serum osteocalcin and glucose metabolism parameters in liver transplantation recipients. A total of 187 liver transplantation patients were cross-sectionally studied, 54 of them developed NODAT. None had been diagnosed of diabetes mellitus prior to transplant. In 133 nondiabetic patients, a 75 g oral glucose tolerance test (OGTT) was performed to assess blood glucose, insulin, and C-peptide levels at baseline and 120 min. Serum total osteocalcin was measured at baseline in all patients.After OGTT, 10.5% of LT patients had NODAT criteria, 51.9% showed impaired glucose tolerance, and 37.6% had normal glucose tolerance. Overall, NODAT prevalence was 36.3%. HOMA-IR was significantly higher in NODAT compared with impaired glucose tolerance and normal glucose tolerance groups (pliver transplantation patients and is associated with insulin resistance. In these patients total osteocalcin is inversely associated with plasma glucose level and insulin resistance indexes. PMID:26743527

  12. 降糖消脂片对2型糖尿病大鼠降糖降脂作用的研究%Study of Jiangtang Xiaozhi Tablets on Decreasing Levels of Blood Glucose and Serum Lipid in Rats with Type 2 Diabetes Mellitus

    Institute of Scientific and Technical Information of China (English)

    金龙; 葛争艳; 郭宇洁; 闫爱国; 董小霞; 李宏坤; 任烨; 刘建勋

    2012-01-01

    目的:观察降糖消脂片对2型糖尿病(T2DM)模型大鼠的降糖降脂作用,为临床治疗T2DM提供实验依据.方法:采用高脂高糖饲料喂饲大鼠4周,诱发胰岛素抵抗(IR),再按低剂量链脲佐菌素(streptozotocin,STZ)25 mg·kg-1 ip,诱发高血糖症,造成T2DM大鼠模型.将实验动物分为正常对照组、模型组、降糖消脂片高、中、低(8,4,2 g·kg-1)剂量组、二甲双胍片(0.2 g·kg-1)组和金芪降糖片(2 g·kg-1)组,连续ig给药8周后杀检,测定空腹血糖(FBG)、总胆固醇(TC)、甘油三酯(TG)、胰岛素(INS)、肿瘤坏死因子(TNF-α)及进行胰腺和肝脏的病理学检查.结果:大鼠在高脂高糖饮食4周时发生高胰岛素血症伴TC增高,成功诱发出IR.STZ ip后造成T2 DM大鼠模型.口服8周后,与模型组比较,降糖消脂片高剂量组FBG明显降低(P<0.01),降糖率达到31.23%,INS水平显著增高(P<0.05),TG明显降低(P<0.01),中剂量组TG及TC明显降低(P<0.01~P<0.05),各剂量组TNF-α有降低的趋势;病理结果显示,与模型组比较,高剂量组胰岛数明显增多(P<0.05),中、低剂量组肝脂肪变性明显改善(P<0.05).结论:降糖消脂片对T2DM模型大鼠有明显的降糖、降脂的作用.%Objective: To explore the effect of Jiangtang Xiaozhi (JTXZ) tablets on decreasing levels of blood glucose and serum lipid in rats with type 2 diabetes and provide the experimental basis for the clinical therapy of type 2 diabetic mellitus (T2DM). Method: The rats were fed on a diet enriched in fat and glucose to induce insulin resistant. And a low dose streptozotocin ( STZ) was injected intrapertoneally (25 mg · kg-1 ) to induce hyperglycemia to establish T2DM. The experimental rats were divided into normal control group, model group, JTXZ tablets groups (8,4 and 2 g ·kg-1 ) , metformin group (0. 2 g·kg-1) and Jinqi Jiangtang tablets group (2 g·kg-1). After all the treatment groups were received their own medicine for 8 weeks

  13. Detection of protein biomarker using a blood glucose meter.

    Science.gov (United States)

    Lan, Tian; Xiang, Yu; Lu, Yi

    2015-01-01

    mHeath technologies are recognized to play important roles in the future of personal care and medicine. However, their full potentials have not been reached, as most of current technologies are restricted to monitoring physical and behavioral parameters, such as body temperature, heart rate, blood pressure, and physical movement, while direct monitoring of biomarkers in body fluids can provide much more accurate and useful information for medical diagnostics. A major barrier to realizing the full potential of mHealth is the high costs and long cycles of developing mHealth devices capable of monitoring biomarkers in body fluids. To lower the costs and shorten the developmental cycle, we have demonstrated the leveraging of the most successful portable medical monitoring device on the market, the blood glucose meter (BGM), with FDA-approved smartphone technologies that allow for wireless transmission and remote monitoring of a wide range of non-glucose targets. In this protocol, an aptamer-based assay for quantification of interferon-γ (IFN-γ) using an off-the-shelf BGM is described. In this assay, an aptamer-based target recognition system is employed. When IFN-γ binds to the aptamer, it triggers the release of a reporter enzyme, invertase, which can catalyze the conversion of sucrose (not detected by BGM) to glucose. The glucose being produced is then detected using a BGM. The system mimics a competitive enzyme-linked immunosorbent assay (ELISA), where the traditional immunoassay is replaced by an aptamer binding assay; the reporter protein is replaced by invertase, and finally the optical or fluorescence detector is replaced with widely available BGMs. PMID:25626534

  14. Capillary bedside blood glucose measurement in neonates: missing a diagnosis of galactosemia.

    Science.gov (United States)

    Özbek, Mehmet Nuri; Öcal, Murat; Tanrıverdi, Sibel; Baysal, Birsen; Deniz, Ahmet; Öncel, Kahraman; Demirbilek, Hüseyin

    2015-03-01

    A number of factors may lead to inaccuracy in measurement of capillary blood glucose with a glucometer. Measurement of other carbohydrate molecules such as galactose and fructose along with glucose can potentially be a cause of error. We report a newborn patient who was referred to our hospital with conjugated bilirubinemia, hepatomegaly and high capillary blood glucose levels measured with a glucometer. Simultaneous biochemical measurements revealed normal blood glucose levels. Further investigation led to a diagnosis of classical galactosemia. Capillary blood glucose level measured with glucometer also dropped to normal values following cessation of breastfeeding and initiation of feeding with a lactose-free formula. PMID:25800483

  15. Effects of Cinnamomum zeylanicum (Ceylon cinnamon on blood glucose and lipids in a diabetic and healthy rat model

    Directory of Open Access Journals (Sweden)

    Priyanga Ranasinghe

    2012-01-01

    Full Text Available Objectives: To evaluate short- and long-term effects of Cinnamomum zeylanicum on food consumption, body weight, glycemic control, and lipids in healthy and diabetes-induced rats. Materials and Methods: The study was conducted in two phases (Phase I and Phase II, using Sprague-Dawley rats in four groups. Phase I evaluated acute effects on fasting blood glucose (FBG (Groups 1 and 2 and on post-oral glucose (Groups 3 and 4 blood glucose. Groups 1 and 3 received distilled-water and Groups 2 and 4 received cinnamon-extracts. Phase II evaluated effects on food consumption, body weight, blood glucose, and lipids over 1 month. Group A (n = 8, distilled-water and Group B (n = 8, cinnamon-extracts were healthy rats, while Group C (n = 5, distilled-water and Group D (n = 5, cinnamon-extracts were diabetes-induced rats. Serum lipid profile and HbA1c were measured on D-0 and D-30. FBG, 2-h post-prandial blood glucose, body weight, and food consumption were measured on every fifth day. Results: Phase I: There was no significant difference in serial blood glucose values in cinnamon-treated group from time 0 (P > 0.05. Following oral glucose, the cinnamon group demonstrated a faster decline in blood glucose compared to controls (P < 0.05. Phase II: Between D0 and D30, the difference in food consumption was shown only in diabetes-induced rats (P < 0.001. Similarly, the significant difference following cinnamon-extracts in FBG and 2-h post-prandial blood glucose from D0 to D30 was shown only in diabetes-induced rats. In cinnamon-extracts administered groups, total and LDL cholesterol levels were lower on D30 in both healthy and diabetes-induced animals (P < 0.001. Conclusions: C. zeylanicum lowered blood glucose, reduced food intake, and improved lipid parameters in diabetes-induced rats.

  16. Enzyme immunoassay of immunoreactive trypsin in serum and blood spots

    International Nuclear Information System (INIS)

    An enzyme immunoassay method for the assay of serum immunoreactive trypsin (IRT) is described. The method is a two site binding assay carried out on microtitre plates as the solid phase. Wells were coated with affinity purified anti-human trypsin and bioinylated anti-trypsin and avidin-β-galactosidase were used as the second antibody and detection system respectively. The assay was sensitive enough to determine IRT concentrations in either serum or dried blood spots. A good correlation was obtained when the method was compared with the Hoechst radioimmunoassay method. (Author)

  17. Association of fasting serum insulin and fasting serum glucose levels with cognitive impairment in Chinese nonagenarians/centenarians

    OpenAIRE

    Yan-Ling, Zhang; Chang-Quan, Huang; Li, Yong; Bi-Rong, Dong

    2013-01-01

    In this study, we examined the association of fasting serum insulin (FSI) and fasting serum glucose (FSG) with cognitive impairment in the very elderly using a sample of Chinese nonagenarians/centenarians. This study used data from a survey that was conducted in 2005 on all residents aged 90 years or more in a district with 2,311,709 inhabitants. FSG, FSI, and cognitive function were analyzed. The sample included 661 unrelated Chinese individuals (aged 90–108 years; mean, 93.52 ± 3.37 years; ...

  18. Effect of Consumption of Tribulus Terrestris on Serum Glucose and Lipid Levels in Diabetic Rats

    OpenAIRE

    M. Roghani; T Baluchnejad Mojarrad; N Andalibi; F Ansari; M Sharayeli

    2010-01-01

    Introduction: The effect of Tribulus terrestris (TT) on serum glucose and lipid levels was investigated in an experimental model of diabetes mellitus in rats. Methods: Female Wistar rats were divided into control, TT-treated control, diabetic, glibenclamide-treated, and TT-treated diabetic groups. For induction of diabetes, streptozotcin (STZ) was administered (60 mg/Kg). Meanwhile, TT-treated groups received TT mixed with standard pelleted food at a weight ratio of 6.25% for 6 weeks. Serum g...

  19. Serum Macrominerals, Glucose and Triglycerides in Arabian Mares during Different Phases of Reproduction Cycle

    OpenAIRE

    Samia Meliani*, Bouabdellah Benallou, Miloud Halbouche1, Abdellatif Niar and Abdelkarim Naceri2

    2011-01-01

    The aim of this study was to determine the difference in biochemical serum constituents of Arabian mares during different phases of the reproduction cycle. According to there reproductive status, mares were assigned to one of the following six groups, i.e., filly (n=5), late pregnancy (n=7), early pregnancy (n=11), estrual mares (n=8), infertile mares (n=8) and mares in post partum less than 8 days (n=4). Serum calcium, phosphorus, sodium, potassium, magnesium, glucose and triglycerides were ...

  20. Effect of Dehulling and Cooking of Lentils (Lens Culinaris, L.)on Serum Glucose and Lipoprotein Levels in Streptozotocin-Induced Diabetic Rats.

    Science.gov (United States)

    Al-Tibi, Amro Mh; Takruri, Hamed R; Ahmad, Mousa N

    2010-12-01

    This study was conducted to investigate the effect of lentils on serum glucose and serum lipid levels in diabetic rats. Forty adult male Sprague-Dawley rats, 12 weeks of age weighing 220-290g, were used. Diabetes was induced by streptozotocin at a level of 35 mg/kg intra-peritoneally. The animals were randomly divided into five groups, eight animals each: a casein diet (control), raw whole lentil (RWL), cooked whole lentil (CWL), raw dehulled lentil (RDL) and cooked dehulled lentil (CDL). Animals were fed with experimental diets for six weeks, sacrificed and blood samples were taken. Serum glucose level of the CDL group (387.9 ± 53.3 mg/dl) was significantly lower (PRDL and RWL groups (529.0 ± 11.7, 538.6 ± 45.0, 542.1 ± 32.2 mg/dl respectively). In addition, HDL concentration of CWL group (66.3 ± 1.9 mg/dl) was significantly higher (PRDL groups (54.9 ± 3.5, 50.8 ± 4.2, 54.0 ± 3.4 mg/dl respectively). However, there was no significant difference in serum glucose and serum HDL between the CDL and CWL groups. No significant differences (p>0.05) were detected in triglycerides, total cholesterol and LDL cholesterol among the experimental groups. It is concluded that cooked lentils rather than raw lentils was more effective in lowering blood glucose and improving HDL cholesterol in diabetic rats. There was no difference between whole and dehulled lentils with regard to effects on blood glucose and HDL cholesterol levels. PMID:22691994

  1. Changes of blood serum in acute cerebral infarction after treatment

    International Nuclear Information System (INIS)

    Objective: To investigate the changes of serum of HDL, LDL, TXB2, 6-K-PGF1α, TG and β-LP in acute cerebral infarction after treatment. Method: The serum levels of HDL, LDL, TXB2, 6-K-PGF1α, TG and β-LP in 94 acute cerebral infarction patients were detected by RIA and biochemistry assay respectively. Results: The results showed that the serum HDL and 6-K-PGF1α levels in acute cerebral infarction patients were increased (P2 were decreased (P<0.01) after treatment. Conclusion: The Hyper coagulable state and blood fat index in acute cerebral infarction after treatment could improve obviously. (authors)

  2. Modeling and Measurement of Correlation between Blood and Interstitial Glucose Changes

    Science.gov (United States)

    Shi, Ting; Li, Dachao; Li, Guoqing; Zhang, Yiming; Xu, Kexin; Lu, Luo

    2016-01-01

    One of the most effective methods for continuous blood glucose monitoring is to continuously measure glucose in the interstitial fluid (ISF). However, multiple physiological factors can modulate glucose concentrations and affect the lag phase between blood and ISF glucose changes. This study aims to develop a compensatory tool for measuring the delay in ISF glucose variations in reference to blood glucose changes. A theoretical model was developed based on biophysics and physiology of glucose transport in the microcirculation system. Blood and interstitial fluid glucose changes were measured in mice and rats by fluorescent and isotope methods, respectively. Computer simulation mimicked curves were fitted with data resulting from fluorescent measurements of mice and isotope measurements of rats, indicating that there were lag times for ISF glucose changes. It also showed that there was a required diffusion distance for glucose to travel from center of capillaries to interstitial space in both mouse and rat models. We conclude that it is feasible with the developed model to continuously monitor dynamic changes of blood glucose concentration through measuring glucose changes in ISF with high accuracy, which requires correct parameters for determining and compensating for the delay time of glucose changes in ISF.

  3. A Simple Laboratory Experiment to Determine the Kinetics of Mutarotation of D-Glucose Using a Blood Glucose Meter

    Science.gov (United States)

    Perles, Carlos E.; Volpe, Pedro L. O.

    2008-01-01

    A simple commercial blood glucose meter is used to follow the kinetics of mutarotation of D-glucose in aqueous solution. The results may be compared with those obtained using an automatic polarimeter, if this is available This experiment is proposed for use by students in a general chemistry, biology, organic chemistry, and physical chemistry…

  4. Analysis on the levels of blood glucose,triglyceride and serum thyroid hormone in patients with schizo phrenia%精神分裂症患者药物治疗过程中血糖三酰甘油和甲状腺素水平分析

    Institute of Scientific and Technical Information of China (English)

    张伟英; 张毓茂; 郑华珠; 江梅英; 郭玉花; 黄兰芳

    2008-01-01

    Objective To confer the leves of blood glucose(GLU) and triglyceride(TG)and serum thyroid hormone in patients with schizophrenia in the cause of treatment.Methods To survey 88 patients with schizophrenia,and assess sera concentration of GLU and TG and thyroid hormone before treatment and during week 2,week 4,week 8,week 12.Results In the cause of treatments,the levels of GLU were up-regulated slightly(P>O.05).The levels of TG rose obviously in the clozapine group (P0.05).治疗4周后,TG水平都显著升高,氯氮平组差异有统计学意义(P<0.01),利培酮组差异也有统计学意义(P<0.05).在治疗8周后,氯氮平组TG水平进一步升高(P<0.001),利培酮组TG水平也明显升高(P<0.01),此时两组FT,水平都显著降低(P<0.05),而TSH水平却显著升高(P<0.05),且患者TG与FT3、FT4水平呈显著负相关.结论 长期服用氯氮平和利培酮治疗的精神分裂症患者存在脂代谢紊乱,而这种紊乱与甲状腺素水平有显著的相关性.

  5. Early blood glucose profile and neurodevelopmental outcome at two years in neonatal hypoxic-ischaemic encephalopathy

    OpenAIRE

    Dempsey Eugene M; Boylan Geraldine B; Murray Deirdre M; Nadeem Montasser; Ryan Cornelius A

    2011-01-01

    Abstract Background To examine the blood glucose profile and the relationship between blood glucose levels and neurodevelopmental outcome in term infants with hypoxic-ischaemic encephalopathy. Methods Blood glucose values within 72 hours of birth were collected from 52 term infants with hypoxic-ischaemic encephalopathy. Hypoglycaemia [ 150 mg/dL (8.3 mmol/L)] were correlated to neurodevelopmental outcome at 24 months of age. Results Four fifths ...

  6. EFFECT OF FIBER SUPPLEMENT ON BLOOD GLUCOSE LEVEL OF NIDDM SUBJECTS

    OpenAIRE

    Seema V Medhe* and Mangal V Medhe

    2013-01-01

    An investigation was undertaken to assess effect of fiber rich supplement on blood glucose level of type 2 diabetic patients. Fiber rich supplement was tested on eleven type 2 diabetic subjects for blood glucose lowering effect by undertaking an intervention of two months feeding trials. Results with respect to two months supplementation indicated that the fiber rich supplement was helpful in significantly reducing the fasting and postprandial blood glucose level of subjects. The reduction in...

  7. Age-related memory impairments due to reduced blood glucose responses to epinephrine

    OpenAIRE

    Morris, Ken A.; Chang, Qing; Mohler, Eric G.; Gold, Paul E.

    2009-01-01

    Increases in blood glucose levels are an important component of the mechanisms by which epinephrine enhances memory formation. The present experiments addressed the hypothesis that a dysfunction in the blood glucose response to circulating epinephrine contributes to age-related memory impairments. Doses of epinephrine and glucagon that significantly increased blood glucose levels in young adult rats were far less effective at doing so in two-year-old rats. In young rats, epinephrine and gluco...

  8. The Effects of Blood Glucose Levels on Cognitive Performance: A Review of the Literature

    Science.gov (United States)

    Feldman, Jolene; Barshi, Immanuel

    2007-01-01

    The purpose of this review paper is to discuss the research literature on the effects of blood glucose levels on executive and non-executive functions in humans. The review begins with a brief description of blood glucose, how it has been studied, previous syntheses of prior studies, and basic results regarding the role of blood glucose on cognitive functioning. The following sections describe work that investigated the effect of blood glucose on both non-executive and executive functions (e.g., sensory processing, psychomotor functioning, attention, vigilance, memory, language and communication, judgement and decision-making, and complex task performance). Within each section, summaries of the findings and challenges to the literature are included. Measurement conversions of blood glucose levels, blood glucose values, and associated symptoms are depicted. References to the types of tests used to investigate blood glucose and cognitive performance are provided. For more detailed descriptions of references within (and in addition to) this paper, an annotated bibliography is also provided. Several moderator variables including individual differences and contextual variables related to the effects of blood glucose levels on performance (e.g., age, gender, time of day, familiarity with the task and symptom awareness, expectancy effects, dose dependent effects, time dependent effects, task specific effects, rising and falling blood glucose levels, and speed and/or accuracy trade-offs) are addressed later in the paper. Some suggestions for future experimental methodologies are also made.

  9. A comprehensive compartmental model of blood glucose regulation for healthy and type 2 diabetic subjects

    DEFF Research Database (Denmark)

    Vahidi, O; Kwok, K E; Gopaluni, R B;

    2016-01-01

    We have expanded a former compartmental model of blood glucose regulation for healthy and type 2 diabetic subjects. The former model was a detailed physiological model which considered the interactions of three substances, glucose, insulin and glucagon on regulating the blood sugar. The main...... drawback of the former model was its restriction on the route of glucose entrance to the body which was limited to the intravenous glucose injection. To handle the oral glucose intake, we have added a model of glucose absorption in the gastrointestinal tract to the former model to address the resultant...... variations of blood glucose concentrations following an oral glucose intake. Another model representing the incretins production in the gastrointestinal tract along with their hormonal effects on boosting pancreatic insulin production is also added to the former model. We have used two sets of clinical data...

  10. Increasing Synthetic Serum Substitute (SSS) Concentrations in P1 Glucose/Phosphate-Free Medium Improves Implantation Rate: A Comparative Study

    OpenAIRE

    Ben-Yosef, Dalit; Yovel, Israel; Schwartz, Tamar; Azem, Fuad; Lessing, Joseph B.; Amit, Ami

    2001-01-01

    Purpose: To assess the comparative efficacy of IVF medium (MediCult, with 5.2 mM glucose) and a glucose/phosphate-free medium, P1 (Irvine Scientific), and to investigate the influence of increasing the serum supplementation (synthetic serum substitute; SSS; Irvine Scientific) to P1 on embryo development and implantation.

  11. Effect of Ethanolic Extract of Fragaria Vesca on serum glucose levels and body weight in diet induced obese rats

    Directory of Open Access Journals (Sweden)

    Venkat ramana Yella

    2015-10-01

    Full Text Available Objective: to evaluate the effect of ethanolic extract Fragaria Vesca on serum glucose levels in diet induced obese rats.Material and methods: Male Wister albino rats weighing 200- 250 gm, were divided into 3 groups of 6 animals each. The animals of all the groups except normal group were given a lipid diet consisting of cholesterol (1%, cholic acid (0.5%, casein (20%, choline (0.25%, d-l-methionin1(0.4%, coconut oil (25%, multi vitamin mix (3.5% and sucrose (48.4% with standard pellet diet for 30 days [20]. Growth rate was monitored during the treatment. Results: There was significantly decrease in blood glucose in standard group compared to HFD model (P< 0.05.  But there was no significant change among other groupsConclusion:  There was no significant change in the blood glucose level in all the groups except the standard group, but there was reduction in body weight.

  12. Application of transcutaneous diffuse reflectance spectroscopy in the measurement of blood glucose concentration

    Institute of Scientific and Technical Information of China (English)

    Wenliang Chen(陈文亮); Rong Liu(刘蓉); Houxin Cui(崔厚欣); Kexin Xu(徐可欣); Lina Lü(吕丽娜)

    2004-01-01

    In this paper, the propagation characteristics of near-infrared (NIR) light in the palm tissue are analyzed,and the principle and feasibility of using transcutaneous diffuse reflectance spectroscopy for non-invasive blood glucose detection are presented. An optical probe suitable for measuring the diffuse reflectance spectrum of human palm and a non-invasive blood glucose detection system using NIR spectroscopy are designed. Based on this system, oral glucose tolerance tests are performed to measure the blood glucose concentrations of two young healthy volunteers. The partial least square calibration model is then constructed by all individual experimental data. The final result shows that correlation coefficients of the two experiments between the predicted blood glucose concentrations and the reference blood glucose concentrations are 0.9870 and 0.9854, respectively. The root mean square errors of prediction of full cross validation are 0.54 and 0.52 mmol/1, respectively.

  13. Effect of local corticosteroid injection of the hand and wrist on blood glucose in patients with diabetes mellitus.

    Science.gov (United States)

    Catalano, Louis W; Glickel, Steven Z; Barron, O Alton; Harrison, Richard; Marshall, Astrid; Purcelli-Lafer, Marissa

    2012-12-01

    Locally administered corticosteroids are a common therapy in many hand and wrist disorders. Corticosteroids pose a theoretical risk to patients with diabetes mellitus by potentially raising blood glucose to hyperglycemic levels. Although oral corticosteroids are known to have an effect on blood glucose control, limited data exist on extra-articular administration. The purpose of this study was to examine the systemic impact of extra-articularly administered corticosteroids in the hand and wrist on serum glucose concentration in patients with diabetes mellitus.Twenty-three patients with diabetes mellitus received a 1-mL triamcinolone acetonide injection for de Quervain's tenosynovitis, trigger finger, flexor carpi ulnaris tendonitis, or carpal tunnel syndrome. Patients recorded their daily morning blood glucose levels for 1 week before injection and for 4 weeks after injection. Average blood glucose levels increased slightly from baseline after injection, reaching statistical significance 1, 5, and 6 days after injection, but were not clinically significant (average increase, 14.2, 9.7, and 32.7 mg/dL, respectively). Isolated increases more than 2 times the standard deviation of preinjection values occurred at least once in the majority of patients. The frequency of hyperglycemic episodes increased after injection, but the proportions of patients with at least 1 hyperglycemic episode before and after injection were not significantly different.These results suggest that local corticosteroid injections are a clinically safe treatment option for inflammatory processes of the hand and wrist in patients with diabetes mellitus. On average, patients experienced slight increases in blood glucose after receiving an injection. Most experienced isolated increases substantially beyond baseline and isolated hyperglycemic effects, but these did not pose an apparent clinical risk. PMID:23218632

  14. Effect of Consumption of Tribulus Terrestris on Serum Glucose and Lipid Levels in Diabetic Rats

    Directory of Open Access Journals (Sweden)

    M Roghani

    2010-03-01

    Full Text Available Introduction: The effect of Tribulus terrestris (TT on serum glucose and lipid levels was investigated in an experimental model of diabetes mellitus in rats. Methods: Female Wistar rats were divided into control, TT-treated control, diabetic, glibenclamide-treated, and TT-treated diabetic groups. For induction of diabetes, streptozotcin (STZ was administered (60 mg/Kg. Meanwhile, TT-treated groups received TT mixed with standard pelleted food at a weight ratio of 6.25% for 6 weeks. Serum glucose and lipid levels were determined before the study and at the 3rd and 4th week after the study. Results: Serum glucose was significantly lower in TT-treated diabetic rats at 3rd and 6th weeks as compared to untreated diabetics (p<0.01 and p<0.005, respectively. In addition, serum total cholesterol, triglyceride, and LDL-cholesterol showed a significant reduction in TT-treated diabetic rats as compared to untreated diabetics (p<0.05. On the other hand, HDL-cholesterol level did not change significantly in TT-treated diabetic group as compared to untreated diabetic group. Conclusions: Oral administration of TT has a significant hypoglycemic effect and in long term leads to appropriate changes in serum LDL-cholesterol, total cholesterol, and triglyceride levels, but does not affect HDL-cholesterol levels in diabetic rats.

  15. Blood serum components and serum protein test of Hybro-PG broilers of different ages

    Directory of Open Access Journals (Sweden)

    PRL Silva

    2007-12-01

    Full Text Available Blood serum samples of HYBRO PG broilers were analyzed, with 30 samples collected from 21-day-old broilers (G1, 30 from 35-day-old birds (G2, and 30 from 42-day-old birds (G3, with the aim of establishing normal values of some blood serum parameters. The activities of the enzymes gamma-glutamyl-transferase (GGT, aspartate aminotransferase (AST, creatine kinase (CK, alkaline phosphatase (ALP, and lactate dehydrogenase (LDH, serum levels of total calcium, calcium ion, phosphorus, sodium, potassium, magnesium, chlorides, creatinine, uric acid, triglycerides, cholesterol, total protein, albumin, total and indirect and direct bilirubin, and electrophoretic profile of serum proteins in acrylamide (SDS-PAGE and agarose gel were determined. There was no influence of age on total bilirubin and albumin levels. All the other evaluated parameters presented differences in at least one age group. Protein electrophoretic profile also changed as a function of age. The obtained results can be considered as normal for the studied ages, and therefore be used as references for the interpretation of laboratory exams of broilers of this genetic line in the evaluated ages.

  16. Parsimonious model for blood glucose level monitoring in type 2 diabetes patients.

    Science.gov (United States)

    Zhao, Fang; Ma, Yan Fen; Wen, Jing Xiao; DU, Yan Fang; Li, Chun Lin; Li, Guang Wei

    2014-07-01

    To establish the parsimonious model for blood glucose monitoring in patients with type 2 diabetes receiving oral hypoglycemic agent treatment. One hundred and fifty-nine adult Chinese type 2 diabetes patients were randomized to receive rapid-acting or sustained-release gliclazide therapy for 12 weeks. Their blood glucose levels were measured at 10 time points in a 24 h period before and after treatment, and the 24 h mean blood glucose levels were measured. Contribution of blood glucose levels to the mean blood glucose level and HbA1c was assessed by multiple regression analysis. The correlation coefficients of blood glucose level measured at 10 time points to the daily MBG were 0.58-0.74 and 0.59-0.79, respectively, before and after treatment (P<0.0001). The multiple stepwise regression analysis showed that the blood glucose levels measured at 6 of the 10 time points could explain 95% and 97% of the changes in MBG before and after treatment. The three blood glucose levels, which were measured at fasting, 2 h after breakfast and before dinner, of the 10 time points could explain 84% and 86% of the changes in MBG before and after treatment, but could only explain 36% and 26% of the changes in HbA1c before and after treatment, and they had a poorer correlation with the HbA1c than with the 24 h MBG. The blood glucose levels measured at fasting, 2 h after breakfast and before dinner truly reflected the change 24 h blood glucose level, suggesting that they are appropriate for the self-monitoring of blood glucose levels in diabetes patients receiving oral anti-diabetes therapy. PMID:25073916

  17. Dietary thylakoids suppress blood glucose and modulate appetite-regulating hormones in pigs exposed to oral glucose tolerance test

    DEFF Research Database (Denmark)

    Montelius, Caroline; Szwiec, Katarzyna; Kardas, Marek;

    2014-01-01

    , either with or without addition of 0.5 g/kg body weight of thylakoid powder. RESULTS: The supplementation of thylakoids to the oral glucose tolerance test resulted in decreased blood glucose concentrations during the first hour, increased plasma cholecystokinin concentrations during the first two hours......BACKGROUND & AIMS: Dietary chloroplast thylakoids have previously been found to reduce food intake and body weight in animal models, and to change metabolic profiles in humans in mixed-food meal studies. The aim of this study was to investigate the modulatory effects of thylakoids on glucose...... metabolism and appetite-regulating hormones during an oral glucose tolerance test in pigs fed a high fat diet. METHODS: Six pigs were fed a high fat diet (36 energy% fat) for one month before oral glucose tolerance test (1 g/kg d-glucose) was performed. The experiment was designed as a cross-over study...

  18. Evaluation of blood glucose and thyroid function in Sudanese diabetic patients

    International Nuclear Information System (INIS)

    This study composes of two parts. The first one is a survey of thyroid abnormalities, and the second one is experiment to estimate the level of thyroid hormones (T4, T3, TSH) among diabetic population and the relationship between the level of glucose, thyroid hormones and lipid profile (TC, LDL, TG, HDL) and comparison of the results with non diabetic group. The survey part of the study to determine the thyroid abnormalities, hypothyroidism or hyperthyroidism clinical or subclinical. Also the study group was divided in to two groups according to insulin requirement. Type I is insulin-dependent diabetes mellitus and type II is non insulin-dependent diabetes mellitus. The study subjects selected for this study consist of one hundred Sudanese diabetic patients, they had mean age of 46.51±10.672 years, a mean height of 162.06±10.77 cm and a mean weight of 73.47±14.91 kg. Fifty healthy non-diabetic people without endocrine disease were chosen as controls. Glucose, and thyroid hormones, thyroxine and triiodothyronine (total T4 and T3) and thyroid stimulating hormone (TSH) were measured. In addition hemoglobin (Hb), total cholesterol (TC), triglyceride (TG), high-density lipoproteins (HDL) were also measured in both groups. Low-density lipoprotein (LDL) was calculated for each sample. Physical examinations such as height, weight, and history of diabetes, family history, treatment were recorded in both groups. Determination of serum hormones concentration was carried out using highly sensitive RIA technique. While determination of blood glucose, hemoglobin, and lipid profile was carried out using enzymatic colorimetric method. The results of this study showed that: 13 patients of the population screened had thyroid disease. The commonest diagnosis was sub clinical hyperthyroidism (6%), followed by sub clinical hypothyroidism (5%) and hyperthyroidism (2%). Female patients with diabetes had the highest annual risk of developing thyroid disease but all patient groups had a

  19. 75 FR 2549 - Clinical Accuracy Requirements for Point of Care Blood Glucose Meters; Public Meeting; Request...

    Science.gov (United States)

    2010-01-15

    ...) Clinical accuracy for blood glucose meters, (2) tight glycemic control in clinical settings, and (3... allow for better glycemic control by diabetics than in the past. Glucose meters are not only used by... evaluated by FDA. For example, glucose meters are increasingly being used to achieve tight glycemic...

  20. Elective Percutaneous Coronary Intervention: The Relationship between Preprocedural Blood Glucose Levels and Periprocedural Myocardial Injury

    OpenAIRE

    Madani, Mohsen; Alizadeh, Keivan; Ghazaee, Sepideh Parchami; Zavarehee, Abbas; Abdi, Seifollah; Shakerian, Farshad; salehi, Negar; Firouzi, Ata

    2013-01-01

    Regardless of the diabetic status of patients with coronary artery disease, hyperglycemia and hypoglycemia are adversely associated with cardiovascular events. The relationship between glucose levels and increased mortality risk in acute myocardial infarction has been shown through various glucose metrics; however, there is a dearth of multivariate analysis of the relationship between elective coronary angioplasty and preprocedural blood glucose levels.

  1. Blood cell characteristic, hematological and serum biochemistry values of Painted Stork (Mycteria leucocephala

    Directory of Open Access Journals (Sweden)

    Worapol Aengwanich

    2002-07-01

    Full Text Available Blood cell characteristics, hematological and serum biochemistry values were examined in 10 mature healthy Painted Storks (Mycteria leucocephala (5 males and 5 females from Khow Khoew Open Zoo, Chonburi province, Thailand. The morphology of erythrocytes, leukocytes and thrombocytes were similar to those of other avian species, but oval shaped granules of heterophils were less stained by Giemsa-Wright solution. Erythrocytes, hemoglobin, packed cell volume, mean corpuscular volume, mean corpuscular hemoglobin, mean corpuscular hemoglobin concentration, total white blood cells, percentage of leukocytes (lymphocyte, heterophil, monocyte, eosinophil, basophil, thrombocytes, serum glucose, serum creatinine, serum glutamic oxaloacetic transaminase and serum glutamic pyruvic transaminase values of Painted Storks were 3.24 + 0.82 106cells / μl, 0.82 106cells /μl, 17.67 + 1.56 g/dl, 42.40 + 3.57 %, 139.47 + 38.70 fl, 58.56 + 18.24 g/dl, 41.76 + 2.90 pg, 3.88 + 2.60 104cells /μl, 76.10 + 10.28 %, 10.60 + 9.49 %, 0.00 + 0.00 %, 12.00 + 8.54 %, 1.00 + 1.33 %, and 1.29 + 0.42104cells /μl, 270.50 + 42.85 mg/dl, 6.19 + 1.70 mg/dl, 249.47 + 42.19 IU/L and 124.29 + 8.81 IU/L, respectively. Total erythrocytes and mean corpuscular volume (MCV differed significantly between male and female Painted Stork (P < 0.05.

  2. Comprehensive investigation of postmortem glucose levels in blood and body fluids with regard to the cause of death in forensic autopsy cases.

    Science.gov (United States)

    Chen, Jian-Hua; Michiue, Tomomi; Inamori-Kawamoto, Osamu; Ikeda, Sayuko; Ishikawa, Takaki; Maeda, Hitoshi

    2015-11-01

    The serum glucose level is regulated within a narrow range by multiple factors under physiological conditions, but is greatly modified in the death process and after death. The present study comprehensively investigated glucose levels in blood and body fluids, including pericardial fluid (PCF), cerebrospinal fluid (CSF) and vitreous humor, reviewing forensic autopsy cases (n=672). Right heart blood glucose level was often higher than at other sites, and the CSF glucose level was the lowest, showing greater dissociation in acute/subacute death cases. The glucose level was higher in the diabetic (high HbA1c) than in the non-diabetic (low HbA1c) group at each site (pdeath due to ischemic heart disease. Fatal methamphetamine (MA) abuse, sepsis, malnutrition (starvation) and hypoglycemia due to antidiabetics showed markedly lower blood glucose levels. Ketones in bilateral cardiac blood and PCF were increased in diabetic ketoacidosis and fatal alcohol abuse as well as in most cases of hyperthermia (heatstroke), hypothermia (cold exposure) and malnutrition. These findings suggest that combined analysis of glucose, HbA1c and ketones in blood and body fluids is useful to investigate not only fatal diabetic metabolic disorders but also death processes due to other causes, including alcohol and MA abuse, as well as thermal disorders, sepsis and malnutrition. PMID:26593993

  3. Self-monitoring of tear glucose: the development of a tear based glucose sensor as an alternative to self-monitoring of blood glucose.

    Science.gov (United States)

    La Belle, Jeffrey T; Adams, Anngela; Lin, Chi-En; Engelschall, Erica; Pratt, Breanna; Cook, Curtiss B

    2016-07-28

    Tear glucose sensing for diabetes management has long been sought as an alternative to more invasive self-monitoring of blood glucose (SMBG). However, tear glucose sensors were known to have limitations, including correlation issues with blood glucose due to low sample volume, low concentration of glucose in the tear fluid, and evaporation of the tear sample. An engineering design approach to solve these problems led to the development of an integrated device capable of collecting the tear sample from the ocular surface with little to no stress on the eye, with an extremely low limit of detection, broad dynamic range, and rapid detection and analysis of sample. Here we present the development of a prototypical self-monitoring of tear glucose (SMTG) sensor, summarizing bench studies on the enzymes and their specificity, the development of the fluid capture device and its manufacture and performance and results of system testing in an animal study where safety, lag time and tear glucose to blood glucose correlation were assessed. PMID:27327531

  4. A switching control strategy for the attenuation of blood glucose disturbances

    OpenAIRE

    Markakis, Mihalis G.; Mitsis, Georgios D.; Papavassilopoulos, George P.; Ioannou, Petros A.; Marmarelis, Vasilis Z.

    2011-01-01

    In this computational study we consider a generalized minimal model structure for the intravenously infused insulin–blood glucose dynamics, which can represent a wide variety of diabetic patients, and augment this model structure with a glucose rate disturbance signal that captures the aggregate effects of various internal and external factors on blood glucose. Then we develop a model-based, switching controller, which attempts to balance between optimal performance, reduced computational com...

  5. Dielectric properties of glucose solutions in the millimetre-wave range and control of glucose content in blood

    Science.gov (United States)

    Meriakri, V. V.; Chigrai, E. E.; Kim, D.; Nikitin, I. P.; Pangonis, L. I.; Parkhomenko, M. P.; Won, J. H.

    2007-04-01

    The measurement of the dielectric properties of sugar solutions, as well as blood imitators and blood, in the millimetre-wave range allows one to obtain valuable information on the possibility of real-time control of glucose concentration in blood. These measurements are also of interest for other applications, for example in the wine industry and for the determination of water content in oil, oil products and other liquids.

  6. A Meta-Analysis of Blood Glucose Effects on Human Decision Making

    DEFF Research Database (Denmark)

    Orquin, Jacob Lund; Kurzban, Robert

    2016-01-01

    identified 42 studies relating to 4 dimensions of decision making: willingness to pay, willingness to work, time discounting, and decision style. We did not find a uniform influence of blood glucose on decision making. Instead, we found that low levels of blood glucose increase the willingness to pay and...... willingness to work when a situation is food related, but decrease willingness to pay and work in all other situations. Low levels of blood glucose increase the future discount rate for food; that is, decision makers become more impatient, and to a lesser extent increase the future discount rate for money....... Low levels of blood glucose also increase the tendency to make more intuitive rather than deliberate decisions. However, this effect was only observed in situations unrelated to food. We conclude that blood glucose has domain-specific effects, influencing decision making differently depending on the...

  7. Aqueous extract ofOcimum tenuiflorum decreases levels of blood glucose in induced hyperglycemic tilapia (Oreochromis niloticus)

    Institute of Scientific and Technical Information of China (English)

    Amilcar Arenal; Leonardo Martn; Nestor M Castillo; Dainier de la Torre; Ubaldo Torres; Reinaldo Gonzlez

    2012-01-01

    Objective:To evaluate, in hyperglycemic tilapia [Oreochromis niloticus (O. niloticus)], the effect of this aqueous extract on blood glucose levels.Methods:The hyperglycemia inO. niloticus was induced by adding glucose to fish pond water.An aqueous extract ofOcimum tenuiflorum (O. tenuiflorum) was prepared by boiling fresh leaves and the doses of0,40,80,200 and400 mg per liter of pond water were tested.Results:The blood sugar concentration for tilapia with hyperglycemic induced was an average of50% higher than the control group.The blood glucose levels in tilapia after the induction of hyperglycemia were higher than the control group for 90 min after the treatment.The treatment with the aqueous extract ofO. tenuiflorum dropped the serum glucose level of hyperglycemic tilapia until it was similar to that of the control group and was dose dependent.Conclusions:The results indicated that O. tenuiflorum was endowed with anti-hyperglycemic activity.To our knowledge, this is the first report on the use of fish as a diabetes model to test natural extracts from plants.

  8. Effects of Zinc Supplementation on the Anthropometric Measurements, Lipid Profiles and Fasting Blood Glucose in the Healthy Obese Adults

    Directory of Open Access Journals (Sweden)

    Sepide Mahluji

    2013-02-01

    Full Text Available Purpose: The aim of this study was to assess the effects of zinc supplementation on anthropometric measures, improving lipid profile biomarkers, and fasting blood glucose level in obese people. Methods: This randomized, double- blind clinical trial was carried out on 60 obese participants in the 18-45 age range for one month. The participants were randomly divided into the intervention group, who received 30 mg/d zinc gluconate, and the placebo group who received 30mg/d starch. Anthropometric measurements (body mass index (BMI, weight and waist circumference were recorded before and at the end of study. Lipid profile biomarkers and fasting blood glucose were determined using enzymatic procedure. Analysis of Covariance (ANCOVA test was run to compare the post-treatment values of the two groups, and t-test was conducted to compare within group changes. Results: Serum zinc concentration was increased significantly in intervention group (p=0.024. BMI and body weight was significantly decreased (p=0.030 and p=0.020, respectively. Lipid profile biomarkers and fating blood glucose did not change significantly but triglyceride level was significantly decreased (p=0.006 in the intervention group. Conclusion: The obtained results indicate that zinc supplementation improves BMI, body weight, and triglyceride concentration without considerable effects on lipid profile and glucose level. Zinc can be suggested as a suitable supplementation therapy for obese people, but more studies are needed to verify the results.

  9. Blood glucose distribution and prevalence of diabetes in Hanoi (Vietnam).

    Science.gov (United States)

    Quoc, P S; Charles, M A; Cuong, N H; Lieu, L H; Tuan, N A; Thomas, M; Balkau, B; Simon, D

    1994-04-01

    Few epidemiologic surveys have been performed to assess the prevalence of diabetes in representative samples, and few data are available on the epidemiologic features of diabetes in Southeast Asia. We report the results of a 1990 study performed in the Hanoi area (Vietnam) on 4,912 subjects (95.0% of the eligible population), aged 15 years or over, selected by a stratified random cluster procedure using the 1989 census list. A two-step design was used: 1) screening for diabetes by measuring capillary blood glucose (CBG) before dinner with a Glucometer II device; and 2) for subjects with a CBG measurement of > or = 105 mg/dl, a diagnostic test on the following morning, using a 75-g oral glucose tolerance test and World Health Organization criteria (93.9% of the positive screenees took this test). CBG values before dinner were unimodally distributed and skewed to the right, increasing with age in both sexes. Women had a significantly higher level of age-adjusted CBG than did men before dinner (p or = 27 kg/m2. The subjects living in the urban areas had higher levels of fasting and 2-hour CBG and a higher diabetes prevalence than did the rural inhabitants (relative risk = 1.3; 95% CI 1.04-3.23). Diabetes appears to be a rare disease in the Hanoi area (1.4% for subjects aged 30-64 years, after age standardization using the Segi distribution), affecting women two times as often as men. Typical insulin-dependent (type I) or obese non-insulin-dependent (type II) diabetes mellitus patients are uncommon. PMID:8166132

  10. Blood Glucose Control Using a Novel Continuous Blood Glucose Monitor and Repetitive Intravenous Insulin Boluses: Exploiting Natural Insulin Pulsatility as a Principle for a Future Artificial Pancreas

    Directory of Open Access Journals (Sweden)

    Nils K. Skjaervold

    2013-01-01

    Full Text Available The aim of this study was to construct a glucose regulatory algorithm by employing the natural pulsatile pattern of insulin secretion and the oscillatory pattern of resting blood glucose levels and further to regulate the blood glucose level in diabetic pigs by this method. We developed a control algorithm based on repetitive intravenous bolus injections of insulin and combined this with an intravascular blood glucose monitor. Four anesthetized pigs were used in the study. The animals developed a mildly diabetic state from streptozotocin pretreatment. They were steadily brought within the blood glucose target range of 4.5–6.0 mmol/L in 21 to 121 min and kept within that range for 128 to 238 min (hypoglycemic values varied from 2.9 to 51.1 min. The study confirmed our hypotheses regarding the feasibility of this new principle for blood glucose control, and the algorithm was constantly improved during the study to produce the best results in the last animals. The main obstacles were the drift of the IvS-1 sensor and problems with the calibration procedure, which calls for an improvement in the sensor stability before this method can be applied fully in new studies in animals and humans.

  11. 补肾活血中药血清对高糖状态下纯化培养的视网膜神经节细胞活力的影响%Effect of Serum Contained Chinese Drugs for Nourishing Shen and Activating Blood on Activity of Purified Retinal Ganglion Cells Cultured in High Glucose Medium

    Institute of Scientific and Technical Information of China (English)

    马荣; 谢学军; 万李; 马殿伟

    2009-01-01

    目的 探讨补肾活血中药血清对体外高糖状态下纯化培养的视网膜神经节细胞活力的影响.方法 体外纯化SD大鼠视网膜神经节细胞(retinal ganglion cells,RGCs),模拟稳定高糖(50 mmol/L)及糖波动环境进行培养,以补肾活血中药血清进行干预,检测RGCs乳酸脱氢酶(lactate dehydrogenase,LDH)漏出量,以推测RGCs活力.结果 RGCs的LDH漏出量(U/L)稳定高糖组24 h(1 349.17±215.50)、48 h(1 220.24±124.53)及72 h(1 982.14±219.03)均较正常对照组(1 628.10±122.10、1 484.13±127.55及2 155.75±140.44)降低(P<0.05);而糖波动组的LDH漏出量在72 h(2 299.60±88.35)较正常对照组增加(P<0.05),且糖波动组各时段的LDH漏出量均较稳定高糖组增加(P<0.05);稳定高糖中药干预组的LDH漏出量在72 h(1 797.62±146.40)时较稳定高糖组减少(P<0.05);糖波动中药干预组的LDH漏出量在48 h(1 259.92±87.74)和72 h(1 940.40±155.47)时均较糖波动组减少(P<0.05).结论 糖波动能明显降低RGCs细胞膜的稳定性,增加细胞膜通透性,降低细胞活力;补肾活血中药血清能降低本实验中稳定高糖及糖波动条件下RGCs的细胞膜通透性,提高细胞膜稳定性,增强其细胞活力,这可能是补肾活血中药复方防治糖尿病视网膜病变(diabetic retinopathy,DRP)的药物干预途径之一.%Objective To investigate the effect of serum contained Chinese drugs for nourishing Shen and activating blood(S-NSAB)on activity of purified retinal ganglion cells(RGCs)cultured in high glucose medium.Methods Purified RGCs of SD rats were cultured in simulative stable high glucose(50 mmol/L)condition(SHG)and fluctuated glucose condition(FGC)separately,they were intervened with S-NSAB,and the lactate dehydrogenase(LDH)leakage was detected by spectrophotometer for estimating the activity of RGCs.Results LDH leakage(U/L)in SHG culture was 1 349.17±215.50 at 24 h,1 220.24±124.53 at 48 h and 1 982.14±219.03 at 72 h,all significantly lower

  12. Protective effect of Cassia glauca Linn. on the serum glucose and hepatic enzymes level in streptozotocin induced NIDDM in rats

    Directory of Open Access Journals (Sweden)

    Farswan Mamta

    2009-01-01

    Full Text Available Objective: The objective of the present study was to investigate the hypoglycemic and hepatoprotective effect of Cassia glauca leaf extracts on normal and non insulin dependent diabetes mellitus (NIDDM in rats. The study was further carried out to investigate the effect of different fractions of the active extract of Cassia glauca, on normal and NIDDM rats, and the effect of active fraction on the blood glucose and hepatic enzymes level. Methods: Diabetes was induced by streptozotocin (STZ at a dose of 90mg/kg, i.p. in neonates. Different extracts of cassia glauca (100mg/kg, p.o. were administered to the diabetic rats. Acetone extract was found to lower the serum glucose level significantly in diabetic rats. Further, the acetone extract was subjected to column chromatography and four fractions were obtained on the basis of TLC. All the four fractions (100mg/kg, p.o. were administered to the diabetic rats. Fraction 1 (F1 caused the maximum reduction in the blood glucose level. The results of the test were compared with the standard antidiabetic drug glibenclamide (5mg/kg, p.o.. Results: Fraction 1 of acetone extract caused a significant reduction in the levels of hepatic enzyme Aspartate transaminase (AST, alanine transaminase (ALT, creatine kinase (CK, and lactate dehydrogenase (LDH in STZ-induced diabetic rats. Conclusion: Improvement in the blood sugar level and normalization of liver functions by Cassia glauca indicates that the plant has hepatoprotective potential, along with antidiabetic activity, and it provides a scientific rationale for the use of Cassia glauca as an antidiabetic agent.

  13. Regulation of gonococcal sialyltransferase, lipooligosaccharide, and serum resistance by glucose, pyruvate, and lactate.

    OpenAIRE

    McGee, D J; Rest, R F

    1996-01-01

    Strain F62 of Neisseria gonorrhoeae gonococci (GC) is sensitive to normal human serum unless CMP-N-acetylneuraminic acid (CMP-NANA) is present. NANA is transferred primarily to a 4.5-kDa lipooligosaccharide (LOS) structure by a GC sialyltransferase (Stase). We investigated LOS and Stase expression and serum resistance in strain F62 grown in different carbon sources and growth conditions. Pyruvate-grown GC expressed 1.9- to 5.6-fold more Stase activity than did glucose-grown GC, whereas lactat...

  14. Intraoperative blood glucose levels in neurosurgical patients : an evaluation of two fluid regimens.

    Directory of Open Access Journals (Sweden)

    Swamy M

    2001-10-01

    Full Text Available Based on the evidence that hyperglycaemia aggravates ischaemic cerebral injury, it has been suggested that blood glucose levels be kept within 200 mg/dL during intracranial neurosurgery. Hypoglycaemia, however, can be a serious problem if glucose-containing solutions are avoided during the first four hours, as suggested in some studies. In order to explore the possibility of administering glucose in moderation so that the blood glucose levels are within acceptable limits, but at the same time the risk of hyoglycaemia is eliminated, we compared two intraoperative fluid regimens. Of the 52 neurosurgical patients studied, 32 patients received alternately 500 ml of 5% dextrose in normal saline and Ringer′s lactate (DNS/RL Group and 18 patients received alternately 500 ml of Ringer′s lactate and normal saline (RL/NS Group. Blood glucose concentrations were determined at the end of each unit of fluid, until the patient received 4 units of fluid. In the DNS/RL group, blood glucose values peaked with the administration of each unit of DNS and tended to return towards the baseline with the subsequent RL, but remained higher than the previous control value. In the RL/NS group, there was a progressive increase in blood glucose values throughout the study period, but the increase was not statistically significant. The blood glucose levels were significantly different between the two groups after each unit of fluid except at the end of the fourth unit. Two patients in the RL/NS group had hypoglycaemia. In conclusion, the DNS/RL regimen maintains blood glucose levels within acceptable limits while avoiding the risk of hyperglycaemia. Withholding glucose completely, lowers blood glucose levels, but carries a risk of hypoglycaemia in some patients.

  15. THE EFFECT OF AVOCADO (PERSEA AMERICANA MILL.) LEAVES EXTRACT TOWARDS THE MOUSE'S BLOOD GLUCOSE DECREASE WITH THE GLUCOSE TOLERANCE METHOD

    OpenAIRE

    Shirly Kumala*, Hesty Utami and Wahyu Kartika Sari

    2013-01-01

    Decrease in blood glucose level test with the use of oral glucose tolerance method from Avocado leaves was carried out. Swiss Webster male mice were divided randomly into five groups. They were negative control, positive control (Glipizide 0.013 mg/20 g BW) and three test groups treated with dosage of avocado leaves ethanol extract 0.490 g/kg, 0.980 g/kg and 1.960 g /BW respectively. Experiment was begun with feeding the mice with the test solution followed by feeding glucose solution (1.5 g/...

  16. Pulsed photoacoustic techniques and glucose determination in human blood and tissue

    OpenAIRE

    Zhao, Z.

    2002-01-01

    Abstract Determination of blood glucose level is a frequently occurring procedure in diabetes care. As the most common method involves collecting blood drops for chemical analysis, it is invasive and liable to afflict a degree of pain and cause a skin injury. To eliminate these disadvantages, this thesis focuses on pulsed photoacoustic techniques, which have potential ability in non-invasive blood glucose measurement. The fundamental theory of photoacoustics in liquid and soft tissue ...

  17. Evaluation of the Capillary Blood Glucose Self-monitoring Program

    Directory of Open Access Journals (Sweden)

    Mariana Cristina Augusto

    2014-10-01

    Full Text Available OBJECTIVE: to evaluate the structure, process and results of the Capillary Blood Glucose Self-monitoring Program in a Brazilian city.METHOD: epidemiological, cross-sectional study. The methodological framework of Donabedian was used to construct indicators of structure, process and outcome. A random sample (n = 288 of users enrolled and 96 health professionals who worked in the program was studied. Two questionnaires were used that were constructed for this study, one for professionals and one for users, both containing data for the evaluation of structure, process and outcome. Anthropometric measures and laboratory results were collected by consulting the patients' health records. The analysis involved descriptive statistics.RESULTS: most of the professionals were not qualified to work in the program and were not knowledgeable about the set of criteria for patient registration. None of the patients received complete and correct orientations about the program and the percentage with skills to perform conducts autonomously was 10%. As regards the result indicators, 86.4% of the patients and 81.3% of the professionals evaluated the program positively.CONCLUSION: the evaluation indicators designed revealed that one of the main objectives of the program, self-care skills, has not been achieved.

  18. Protective effect of Hibiscus sabdariffa against serum/glucose deprivation-induced PC12 cells injury

    OpenAIRE

    Elham Bakhtiari; Azar Hosseini; Seyed Hadi Mousavi

    2015-01-01

    Objectives: Findings natural products with antioxidant and antiapoptotic properties has been one of the interesting challenges in the search for the treatment of neurodegenerative diseases including ischemic stroke. Serum/glucose deprivation (SGD) has been used as a model for the understanding of the molecular mechanisms of neuronal damage during ischemia in vitro and for the expansion of neuroprotective drugs against ischemia-induced brain injury. Recent studies showed that Hibiscus sabdarif...

  19. Plasma Glucose and Serum Ceruloplasmin in Metabolic Syndrome and Diabetes Mellitus Type 2

    OpenAIRE

    Ashok Kumar Jeppu; Asha Augusthy; Kavitha Ashok Kumar

    2016-01-01

    Diabetes mellitus type 2 and metabolic syndrome are conditions associated with insulin resistance and hyperglycemia. Metabolic syndrome is a risk factor for diabetes mellitus type 2. Plasma glucose (fasting/postprandial) and serum ceruloplasmin levels and their relationship were studied. Study population consisted of 150 individuals—50 individuals with diabetes mellitus type 2, 50 individuals with metabolic syndrome, and 50 age- and sex-matched healthy controls. Plasma levels of fasting and p...

  20. High activity enables life on a high-sugar diet: blood glucose regulation in nectar-feeding bats

    OpenAIRE

    Kelm, Detlev H.; Simon, Ralph; Kuhlow, Doreen; Voigt, Christian C.; Ristow, Michael

    2011-01-01

    High blood glucose levels caused by excessive sugar consumption are detrimental to mammalian health and life expectancy. Despite consuming vast quantities of sugar-rich floral nectar, nectar-feeding bats are long-lived, provoking the question of how they regulate blood glucose. We investigated blood glucose levels in nectar-feeding bats (Glossophaga soricina) in experiments in which we varied the amount of dietary sugar or flight time. Blood glucose levels increased with the quantity of gluco...

  1. The Food and Drug Administration Is Now Preparing to Establish Tighter Performance Requirements for Blood Glucose Monitors

    OpenAIRE

    Klonoff, David C.

    2010-01-01

    On March 16 and 17, 2010, the Food and Drug Administration (FDA) presented a public meeting about blood glucose monitoring at the Gaithersberg Hilton Hotel. The meeting was intended to present expert opinions and solicit input from the public about whether to develop new regulatory policies for blood glucose monitors. The meeting was divided into three sections: (1) Clinical Accuracy Requirements for Blood Glucose Monitors, (2) Interferences and Limitations of Blood Glucose Monitors, and (3) ...

  2. Detection of serum iron and zinc levels in patients with type 2 diabetes mellitus and their relationship with glucose and lipid metabolism

    Institute of Scientific and Technical Information of China (English)

    Xiao-Yong Tang; Wan-Fen Zhong

    2016-01-01

    Objective:To study the blood iron and zinc levels in patients with type 2 diabetes mellitus and their relationship with glucose and lipid metabolism.Methods:Patients with type 2 diabetes mellitus were enrolled in T2DM group and healthy people were enrolled in control group. T2DM patients were divided into low iron and zinc group and high iron and zinc group with the median of serum iron and zinc contents as the standard. Serum trace elements contents and glucose and lipid metabolism indicators were detected.Results:T2DM patients’ blood iron contents were significantly higher than those of the control group while the zinc contents were significantly lower than those of the control group. Copper, calcium and magnesium contents had no significant difference between two groups of patients. HbA1c%, glucagon and HOMA-IR as well as apoB, apoE, ApoM, leptin and vaspin contents in high iron group were higher than those in low iron group while serum insulin, GLP-1, GIP, ApoA1, APN and HOMA-β levels were lower than those in low iron group. HbA1c%, glucagon, HOMA-IR, apoB, apoE, ApoM, leptin and vaspin contents in high zinc group were lower than those in low zinc group while serum insulin, GLP-1, GIP, ApoA1, APN and HOMA-β levels were higher than those in low zinc group.Conclusions: the serum iron level was abnormally high and the zinc content was abnormally low in patients with type 2 diabetes mellitus. Serum iron and zinc levels were closely related to the glucose and lipid metabolism.

  3. The Coupling of Cerebral Metabolic Rate of Glucose and Cerebral Blood Flow In Vivo

    DEFF Research Database (Denmark)

    Hasselbalch, Steen; Paulson, Olaf Bjarne

    2012-01-01

    The energy supplied to the brain by metabolic substrate is largely utilized for maintaining synaptic transmission. In this regulation cerebral blood flow and glucose consumption is tightly coupled as well in the resting condition as during activation. Quantification of cerebral blood flow and...... not used for aerobic metabolism. Although some of the excess glucose uptake can be explained by lactate production, this phenomenon can still not account for the excess glucose uptake. Thus, more complex metabolic patterns in the brain might be reflected in the excess glucose uptake during activation...

  4. Short-term application of low-dose growth hormone in surgical patients: Effects on nitrogen balance and blood glucose

    Institute of Scientific and Technical Information of China (English)

    Ming-Ming Zhang; Xiao-Ting Wu; Yong Zhou; Kun Qian; Ya-Min Zheng

    2007-01-01

    AIM: To investigate the effectiveness and safety of recombinant human growth hormone (rhGH) in postoperative patients.METHODS: A total of 48 consecutive patients undergoing abdominal operations were randomized to receive either subcutaneous rhGH (0.15 IU/kg) or placebo (menstruum) injections daily for 7 d after surgery. The two groups had similar nutritional intake. Blood samples for serum fibronectin, albumin, prealbumin, transferrin and the total lymphocyte count, as well as glucose levels were collected to study the rhGH effect. Basal laboratory evaluation, and nutritional status were estimated on d 1before as baseline and d 3 and 10 after operation using standard laboratory techniques. Nitrogen balance was measured from d 3 to 9 after operation.RESULTS: The cumulative nitrogen balance was significantly improved in rhGH group compared with the placebo group (11.37±16.82 vs -9.11±17.52, P = 0.0003).Serum fibronectin was also significantly higher in the rhGH group than in the placebo group (104.77±19.94vs 93.03±16.03, P<0.05), whereas changes in serum albumin, prealbumin, transferrin and total lymphocyte counts were not statistically significant. Mean blood glucose levels were significantly higher in the rhGH group from d 3 to 6 after operation.CONCLUSION: If blood glucose can be controlled, lowdose growth hormone together with hypocaloric nutrition is effective on improving positive nitrogen balance and protein conservation and safe is in postoperative patients.

  5. Glucagon-like peptide-1 inhibits blood-brain glucose transfer in humans

    DEFF Research Database (Denmark)

    Lerche, Susanne; Brock, Birgitte; Rungby, Jørgen;

    2008-01-01

    demonstrated that a hormone involved in postprandial glucose regulation also limits glucose delivery to brain tissue and hence provides a possible regulatory mechanism for the link between plasma glucose and brain glucose. Because GLP-1 reduces glucose uptake across the intact blood-brain barrier at normal...... glycemia, GLP-1 may also protect the brain by limiting intracerebral glucose fluctuation when plasma glucose is increased.......OBJECTIVE: Glucagon-like peptide-1 (GLP-1) has many effects on glucose homeostasis, and GLP-1 receptors are broadly represented in many tissues including the brain. Recent research in rodents suggests a protective effect of GLP-1 on brain tissue. The mechanism is unknown. We therefore tested...

  6. Internet-Based Contingency Management to Improve Adherence with Blood Glucose Testing Recommendations for Teens with Type 1 Diabetes

    Science.gov (United States)

    Raiff, Bethany R.; Dallery, Jesse

    2010-01-01

    The current study used Internet-based contingency management (CM) to increase adherence with blood glucose testing to at least 4 times daily. Four teens diagnosed with Type 1 diabetes earned vouchers for submitting blood glucose testing videos over a Web site. Participants submitted a mean of 1.7 and 3.1 blood glucose tests per day during the 2…

  7. High glucose impairs superoxide production from isolated blood neutrophils

    DEFF Research Database (Denmark)

    Perner, A; Nielsen, S E; Rask-Madsen, J

    2003-01-01

    Superoxide (O(2)(-)), a key antimicrobial agent in phagocytes, is produced by the activity of NADPH oxidase. High glucose concentrations may, however, impair the production of O(2)(-) through inhibition of glucose-6-phosphate dehydrogenase (G6PD), which catalyzes the formation of NADPH. This stud...... measured the acute effects of high glucose or the G6PD inhibitor dehydroepiandrosterone (DHEA) on the production of O(2)(-) from isolated human neutrophils....

  8. Effect of two different regimes of carbohydrate and protein on performance and serum level of insulin and glucose in soccer players

    Directory of Open Access Journals (Sweden)

    M. Hozoori

    2006-07-01

    Full Text Available Background: The aim of this study was to determine the effect of two different regimes, containing different carbohydrate to protein ratios on performance, serum glucose and insulin after exercise in soccer players in Tehran; Iran. Methods: Nineteen male soccer players under training [age = 17/5 +/- 1.5 (SE yr] were selected and completed two sequential trials separated by 1 week, in a paired cross-over study design. In each trial, subjects after running to fatigue; received one of three regimes, using a random- order design as follows: HPRO ( CHO 56%, PRO 19% & fat 25% of total energy; HCHO ( CHO 64%, PRO 11% & fat 25% of total energy or control ( CHO 60%, PRO 15% & fat 25% of total energy. The calorie of 3 regimes were equal. After consumption of meal up to120 min, blood was obtained before and at intervals. After 3 hours athlete performance was measured. Results: The study indicates no significant difference in the serum insulin and glucose response among three regimes (P > 0.05. There was no difference in performance between three regimes after 3 h (p > 0.05. Conclusion: The results suggest that post exercise regimes have no influence on performance, serum glucose and serum insulin. Thus total energy content and carbohydrate content may be important in recovery after exercise.

  9. Application of optical lens of a CD writer for detecting the blood glucose semi-invasively

    Science.gov (United States)

    Meshram, N. D.; Dahikar, P. B.

    2014-10-01

    Recent technological advancements in the photonics industry have led to a resurgence of interest in optical glucose sensing and to realistic progress toward the development of an optical glucose sensor. Such a sensor has the potential to significantly improve the quality of life for the estimated 16 million diabetics in this country by making routine glucose measurements more convenient. Currently over 100 small companies and universities are working to develop noninvasive or minimally invasive glucose sensing technologies, and optical methods play a large role in these efforts. It has become overwhelmingly clear that frequent monitoring and tight control of blood sugar levels are requisite for effective management of Diabetes mellitus and reduction of the complications associated with this disease. The pain and trouble associated with current "finger-stick" methods for blood glucose monitoring result in decreased patient compliance and a failure to control blood sugar levels. Thus, the development of a convenient noninvasive blood glucose monitor holds the potential to significantly reduce the morbidity and mortality associated with Diabetes. A method and apparatus for noninvasive measurement of blood glucose concentration based on transilluminated laser beam via the Index Finger has been reported in this paper. This method depends on photodiode based laser operating at 632.8 nm wavelength. During measurement, the index finger is inserted into the glucose sensing unit, the transilluminated optical signal is converted into an electrical signal, compared with the reference electrical signal, and the obtained difference signal is processed by signal processing unit which presents the results in the form of blood glucose concentration. This method would enable the monitoring blood glucose level of the diabetic patient continuously, safely and noninvasively..

  10. Application of optical lens of a CD writer for detecting the blood glucose semi-invasively

    International Nuclear Information System (INIS)

    Recent technological advancements in the photonics industry have led to a resurgence of interest in optical glucose sensing and to realistic progress toward the development of an optical glucose sensor. Such a sensor has the potential to significantly improve the quality of life for the estimated 16 million diabetics in this country by making routine glucose measurements more convenient. Currently over 100 small companies and universities are working to develop noninvasive or minimally invasive glucose sensing technologies, and optical methods play a large role in these efforts. It has become overwhelmingly clear that frequent monitoring and tight control of blood sugar levels are requisite for effective management of Diabetes mellitus and reduction of the complications associated with this disease. The pain and trouble associated with current “finger-stick” methods for blood glucose monitoring result in decreased patient compliance and a failure to control blood sugar levels. Thus, the development of a convenient noninvasive blood glucose monitor holds the potential to significantly reduce the morbidity and mortality associated with Diabetes. A method and apparatus for noninvasive measurement of blood glucose concentration based on transilluminated laser beam via the Index Finger has been reported in this paper. This method depends on photodiode based laser operating at 632.8 nm wavelength. During measurement, the index finger is inserted into the glucose sensing unit, the transilluminated optical signal is converted into an electrical signal, compared with the reference electrical signal, and the obtained difference signal is processed by signal processing unit which presents the results in the form of blood glucose concentration. This method would enable the monitoring blood glucose level of the diabetic patient continuously, safely and noninvasively.

  11. Application of optical lens of a CD writer for detecting the blood glucose semi-invasively

    Energy Technology Data Exchange (ETDEWEB)

    Meshram, N. D., E-mail: meshramnileshsd@gmail.com [Mathuradas Mohota College of Sciences, Nagpur-440009 (India); Dahikar, P. B., E-mail: pbdahikar@rediffmail.com [Kamla Nehru Mahavidyalaya, Sakkardara Square, Nagpur-440009 (India)

    2014-10-15

    Recent technological advancements in the photonics industry have led to a resurgence of interest in optical glucose sensing and to realistic progress toward the development of an optical glucose sensor. Such a sensor has the potential to significantly improve the quality of life for the estimated 16 million diabetics in this country by making routine glucose measurements more convenient. Currently over 100 small companies and universities are working to develop noninvasive or minimally invasive glucose sensing technologies, and optical methods play a large role in these efforts. It has become overwhelmingly clear that frequent monitoring and tight control of blood sugar levels are requisite for effective management of Diabetes mellitus and reduction of the complications associated with this disease. The pain and trouble associated with current “finger-stick” methods for blood glucose monitoring result in decreased patient compliance and a failure to control blood sugar levels. Thus, the development of a convenient noninvasive blood glucose monitor holds the potential to significantly reduce the morbidity and mortality associated with Diabetes. A method and apparatus for noninvasive measurement of blood glucose concentration based on transilluminated laser beam via the Index Finger has been reported in this paper. This method depends on photodiode based laser operating at 632.8 nm wavelength. During measurement, the index finger is inserted into the glucose sensing unit, the transilluminated optical signal is converted into an electrical signal, compared with the reference electrical signal, and the obtained difference signal is processed by signal processing unit which presents the results in the form of blood glucose concentration. This method would enable the monitoring blood glucose level of the diabetic patient continuously, safely and noninvasively.

  12. The modulatory role of spinally located histamine receptors in the regulation of the blood glucose level in d-glucose-fed mice.

    Science.gov (United States)

    Sim, Yun-Beom; Park, Soo-Hyun; Kim, Sung-Su; Kim, Chea-Ha; Kim, Su-Jin; Lim, Su-Min; Jung, Jun-Sub; Ryu, Ohk-Hyun; Choi, Moon-Gi; Suh, Hong-Won

    2014-02-01

    The possible roles of spinal histamine receptors in the regulation of the blood glucose level were studied in ICR mice. Mice were intrathecally (i.t.) treated with histamine 1 (H1) receptor agonist (2-pyridylethylamine) or antagonist (cetirizine), histamine 2 (H2) receptor agonist (dimaprit) or antagonist (ranitidine), histamine 3 (H3) receptor agonist (α-methylhistamine) or antagonist (carcinine) and histamine 4 (H4) receptor agonist (VUF 8430) or antagonist (JNJ 7777120), and the blood glucose level was measured at 30, 60 and 120 min after i.t. administration. The i.t. injection with α-methylhistamine, but not carcinine slightly caused an elevation of the blood glucose level. In addition, histamine H1, H2, and H4 receptor agonists and antagonists did not affect the blood glucose level. In D-glucose-fed model, i.t. pretreatment with cetirizine enhanced the blood glucose level, whereas 2-pyridylethylamine did not affect. The i.t. pretreatment with dimaprit, but not ranitidine, enhanced the blood glucose level in D-glucose-fed model. In addition, α-methylhistamine, but not carcinine, slightly but significantly enhanced the blood glucose level D-glucose-fed model. Finally, i.t. pretreatment with JNJ 7777120, but not VUF 8430, slightly but significantly increased the blood glucose level. Although histamine receptors themselves located at the spinal cord do not exert any effect on the regulation of the blood glucose level, our results suggest that the activation of spinal histamine H2 receptors and the blockade of spinal histamine H1 or H3 receptors may play modulatory roles for up-regulation and down-regulation, respectively, of the blood glucose level in D-glucose fed model. PMID:24634595

  13. Study the Effects of Ramadan Fasting on the Serum Glucose and Lipid Profile among Healthy Jordanian Students

    Directory of Open Access Journals (Sweden)

    Kama M.S. Mansi

    2007-01-01

    Full Text Available Ramadan is the holiest month in the Islamic calendar and Muslims fast during this month. We designed this study to evaluate the effect of Ramadan fasting on plasma lipids profile and serum glucose among healthy Jordanian students. Ramadan fasting entails major changes in sleep pattern, physical activity and eating habits, which may cause changes in metabolism both in health and disease. This cohort study was performed during Ramadan in October 2006 (Islamic year 1427. The subjects were 70 healthy Jordanian students (male and female from three Jordanian universities who fasted during Ramadan. Their mean age was 21 ±1.6 years. We evaluated some anthropometric parameters as body weight (Kg, pulse rate (per minute and systolic and diastolic blood pressure (mmHg. All parameters at 4 week of Ramadan were significantly lower than pre-Ramadan values, 2 weeks after Ramadan fasting, body weight and other parameters had a trend to recoup to pre-Ramadan status; however, they were still lower than the pre-Ramadan values. We evaluated blood glucose, triglycerides (TG, cholesterol, low-density lipoprotein LDL, high-density lipoprotein (HDL, and Triglycerides at 1 day before, at week 1, 2, 4 of the Ramadan month and after tow weeks after the end of Ramadan. It was found that high-density lipoprotein (HDL cholesterol increased significantly during Ramadan, which indicated positive association with pulse rate and fat intake and negative association with systolic blood pressure and weight. . The LDLc was significantly reduced at the end of fasting. A reduction in the average TC value was observed at the end of fasting but the difference was not statistically and there no significant rise in the TG and blood sugar values at the end of fasting. This study indicated that Ramadan fasting led to a decrease weight, LDLc significantly, and significant not statistically reduction in the average TC value, and significantly increasing in HDLc was noted during Ramadan

  14. Determination of phosphodiesterase I activity in human blood serum.

    Science.gov (United States)

    Hynie, I; Meuffels, M; Poznanski, W J

    1975-09-01

    Phosphodiesterase I (EC 3.1.4.1) activity was detected in normal human blood serum. The enzyme is stable at laboratory temperature for three days, but is inactivated at pH less than 7. The pH for optimum activity increases with the substrate concentration (under the conditions used, from pH 9.0 to 10.2) and, conversely, the Km increases with pH and buffer concentration. The enzyme is inhibited by ethylenediaminetetraacetate but not by phosphate (0.1 mol/liter). We developed a simple quantitative method for its determination, based on hydrolysis of the p-nitrophenyl ester of thymidine 5'-monophosphate and subsequent measurement of the liberated p-nitrophenol at 400 nm in NaOH (0.1 mol/liter). Normal values (mean +/- 2 SD) were determined to be 33 +/- 6.4 U/liter. Preliminary studies indicate that phosphodiesterase I activity is greater than normal in serum of patients with necrotic changes in the liver or kidney or in cases of breast cancer, but not in that of patients with myocardial infarction, bone cancer, lung cancer, or chronic liver cirrhosis. PMID:168991

  15. Dextrose in the banked blood products does not seem to affect the blood glucose levels in patients undergoing liver transplantation

    OpenAIRE

    Cheng, Kwok-Wai; Chen, Chao-Long; Cheng, Yu-Fan; Tseng, Chia-Chih; Wang, Chih-Hsien; Chen, Yaw-Sen; Wang, Chih-Chi; Huang, Tung-Liang; Eng, Hock-Liew; Chiu, King-Wah; Wang, Shih-Hor; Lin, Chih-Che; Lin, Tsan-Shiun; Liu, Yueh-Wei; Jawan, Bruno

    2005-01-01

    AIM: Hyperglycemia commonly seen in liver transplantation (LT) has often been attributed to the dextrose in the storage solution of blood transfusion products. The purpose of the study is to compare the changes of the blood glucose levels in transfused and non-transfused patients during LT.

  16. Effect of pertussis toxin pretreated centrally on blood glucose level induced by stress.

    Science.gov (United States)

    Suh, Hong-Won; Sim, Yun-Beom; Park, Soo-Hyun; Sharma, Naveen; Im, Hyun-Ju; Hong, Jae-Seung

    2016-09-01

    In the present study, we examined the effect of pertussis toxin (PTX) administered centrally in a variety of stress-induced blood glucose level. Mice were exposed to stress after the pretreatment of PTX (0.05 or 0.1 µg) i.c.v. or i.t. once for 6 days. Blood glucose level was measured at 0, 30, 60 and 120 min after stress stimulation. The blood glucose level was increased in all stress groups. The blood glucose level reached at maximum level after 30 min of stress stimulation and returned to a normal level after 2 h of stress stimulation in restraint stress, physical, and emotional stress groups. The blood glucose level induced by cold-water swimming stress was gradually increased up to 1 h and returned to the normal level. The intracerebroventricular (i.c.v.) or intrathecal (i.t.) pretreatment with PTX, a Gi inhibitor, alone produced a hypoglycemia and almost abolished the elevation of the blood level induced by stress stimulation. The central pretreatment with PTX caused a reduction of plasma insulin level, whereas plasma corticosterone level was further up-regulated in all stress models. Our results suggest that the hyperglycemia produced by physical stress, emotional stress, restraint stress, and the cold-water swimming stress appear to be mediated by activation of centrally located PTX-sensitive G proteins. The reduction of blood glucose level by PTX appears to due to the reduction of plasma insulin level. The reduction of blood glucose level by PTX was accompanied by the reduction of plasma insulin level. Plasma corticosterone level up-regulation by PTX in stress models may be due to a blood glucose homeostatic mechanism. PMID:27610033

  17. The effect of euglucaemic hyperinsulinaemia on forearm blood flow and glucose uptake in the human forearm.

    Science.gov (United States)

    Fugmann, A; Lind, L; Andersson, P E; Millgård, J; Hänni, A; Berne, C; Lithell, H

    1998-12-01

    Insulin-mediated stimulation of blood flow to skeletal muscle has been proposed to be of major importance for insulin-mediated glucose uptake. The aim of this study was to investigate the relative importance of blood flow and glucose extraction as determinants of insulin-mediated glucose uptake in the human forearm. Forearm blood flow (FBF), glucose extraction and oxygen consumption were evaluated for 100 min during the euglycaemic hyperinsulinaemic clamp (92 mU/l) in nine healthy subjects. FBF was measured by venous occlusion plethysmography. Forearm glucose uptake increased sevenfold during the hyperinsulinaemia (P<0.001). Forearm glucose extraction showed a minor increase during the first 10 min of hyperinsulinaemia, but the most marked increase took place between 10 and 20 min (+170%). Thereafter, only a minor further increase was seen. During the first 10 min of hyperinsulinaemia FBF was unchanged. Thereafter, FBF increased steadily to a plateau reached after 60 min (+50%, P<0.001). A close relationship between whole body glucose uptake and FBF was seen at the end of the clamp (r = 0.75, P<0.02), but at this time the relationship between whole body glucose uptake and forearm glucose extraction was not significant. The modest increase in O2 consumption seen at the beginning of the clamp (+19%) was not related to FBF during the early phase of the clamp. In conclusion, the early course of insulin-mediated glucose uptake in the human forearm was mainly due to an increase in glucose extraction. However, with time the insulin-mediated increase in blood flow increased in importance and after 100 min of hyperinsulinaemia FBF was the major determinant of glucose uptake. PMID:9934819

  18. Impact of Epidemic Rates of Diabetes on the Chinese Blood Glucose Testing Market

    OpenAIRE

    Hu, Jamie; Zhang, Xian-En

    2011-01-01

    China has become the country with the largest diabetes mellitus population in the world since the 1990s. About 100 million diabetes cases have been diagnosed since 2008. Handheld blood glucose meters and test strips are urgently needed for daily patient measurement. The glucose monitor with a screen-printed carbon-based glucose electrode has been in commercial production since 1994. Since then, approximately 20 companies have been involved in manufacturing and marketing meters and test strips...

  19. Fingertip Blood Capillary Whole Blood Glucose and Venous Plasma Glucose test Results Contrast Analysis%指尖毛细血管全血糖与静脉血浆血糖检测结果对比分析

    Institute of Scientific and Technical Information of China (English)

    邢美荣

    2016-01-01

    Objective To compare the blood glucose and blood glucose in the blood glucose and blood glucose of the finger tip blood glucose and to explore the accuracy of blood glucose detection in the blood glucose of finger tip capillary. Meth-ods From January 2014 to October 2015, 200 cases of blood glucose detection in the center of the disease control depart-ment of our CDC were studied. According to their blood glucose levels, the patients were divided into low blood sugar group, normal group, abnormal glucose tolerance group and diabetic group. Blood glucose was detected by using the automatic bio-chemical analyzer to measure blood glucose of blood and blood glucose in the blood and blood of the finger tip, which were collected from 200 cases of the patients. Results There were no significant differences in blood glucose levels between the blood glucose test results of the blood glucose test of the blood glucose and the blood glucose in the blood glucose of the patients with low blood glucose, glucose tolerance and glucose tolerance test in the patients with low blood glucose and glu-cose tolerance. Conclusion The method of blood glucose detection in blood glucose of finger tip has a high accuracy, and it is close to that of venous blood glucose detection. It can effectively reflect the blood glucose level of the subjects, and can be used as an effective method for the monitoring and management of the community and family.%目的:对比分析指尖毛细血管全血血糖与静脉血浆血糖的检测结果,以探讨指尖毛细血管全血糖检测血糖的准确性。方法于2014年1月-2015年10月,选取在该疾控中心体检科进行血糖检测的200例受检者进行研究,按照其血糖情况进行分组,分为低血糖组、血糖正常组、糖耐量异常组、糖尿病组。分别于空腹状态时和餐后2 h,采集这200例受检者的指尖毛细血管全血和外周静脉血液,使用全自动生化分析仪进行血糖测定,对比指尖毛细血

  20. The effects of food deprivation and incentive motivation on blood glucose levels and cognitive function.

    Science.gov (United States)

    Green, M W; Elliman, N A; Rogers, P J

    1997-11-01

    The current study investigated the relationships between blood glucose levels, mild food deprivation, sympathetic arousal, and cognitive processing efficiency. Subjects (n = 82) were randomly assigned to four experimental conditions, comprising combined manipulations of food deprivation and incentive motivation. Baseline and mid-session measurements of blood glucose, blood pressure and pulse rate were taken. Subjects completed a number of measures of cognitive processing efficiency and self report measures of affective and somatic state. Although glucose levels were lowered following food deprivation, there was no significant detrimental effect of food deprivation on task performance. However, improved recognition memory processing times were associated with deprivation. Incentive motivation was associated with faster simple reaction times and higher diastolic blood pressure. There were no significant relationships between glucose levels and task performance, further supporting the hypothesis that the brain is relatively invulnerable to short food deprivation. PMID:9399371

  1. Polarization Sensitive Optical Coherence Tomography for Blood Glucose Monitoring in Human Subjects

    CERN Document Server

    Solanki, Jitendra; Sen, Pratima; Andrews, Joseph Thomas

    2012-01-01

    A device based on Polarization sensitive optical coherence tomography is developed to monitor blood glucose levels in human subjects. The device was initially tested with tissue phantom. The measurements with human subjects for various glucose concentration levels are found to be linearly dependent on the degree of circular polarization obtainable from the PS-OCT.

  2. Kinetic Parameters for the Noncatalyzed and Enzyme-Catalyzed Mutarotation of Glucose Using a Blood Glucometer

    Science.gov (United States)

    Hardee, John R.; Delgado, Bryan; Jones, Wray

    2011-01-01

    The kinetic parameters for the conversion of alpha-D-glucose to beta-D-glucose were measured using a blood glucometer. The reaction order, rate constant, and Arrhenius activation energy are reported for the noncatalyzed reaction and turnover number and Michaelis constant are reported for the reaction catalyzed by porcine kidney mutarotase. The…

  3. Modelling, verification, and calibration of a photoacoustics based continuous non-invasive blood glucose monitoring system

    Science.gov (United States)

    Pai, Praful P.; Sanki, Pradyut K.; Sarangi, Satyabrata; Banerjee, Swapna

    2015-06-01

    This paper examines the use of photoacoustic spectroscopy (PAS) at an excitation wavelength of 905 nm for making continuous non-invasive blood glucose measurements. The theoretical background of the measurement technique is verified through simulation. An apparatus is fabricated for performing photoacoustic measurements in vitro on glucose solutions and in vivo on human subjects. The amplitude of the photoacoustic signals measured from glucose solutions is observed to increase with the solution concentration, while photoacoustic amplitude obtained from in vivo measurements follows the blood glucose concentration of the subjects, indicating a direct proportionality between the two quantities. A linear calibration method is applied separately on measurements obtained from each individual in order to estimate the blood glucose concentration. The estimated glucose values are compared to reference glucose concentrations measured using a standard glucose meter. A plot of 196 measurement pairs taken over 30 normal subjects on a Clarke error grid gives a point distribution of 82.65% and 17.35% over zones A and B of the grid with a mean absolute relative deviation (MARD) of 11.78% and a mean absolute difference (MAD) of 15.27 mg/dl (0.85 mmol/l). The results obtained are better than or comparable to those obtained using photoacoustic spectroscopy based methods or other non-invasive measurement techniques available. The accuracy levels obtained are also comparable to commercially available continuous glucose monitoring systems.

  4. Optical coherence tomography for glucose monitoring in blood

    Science.gov (United States)

    Ullah, Hafeez; Hussain, Fayyaz; Ikram, Masroor

    2015-08-01

    In this review, we have discussed the potential application of the emerging imaging modality, i.e., optical coherence tomography (OCT) for glucose monitoring in biological tissues. OCT provides monitoring of glucose diffusion in different fibrous tissues like in sclera by determining the permeability rate with acceptable accuracy both in type 1 and in type 2 diabetes. The maximum precision of glucose measurement in Intralipid suspensions, for example, with the OCT technique yields the accuracy up to 4.4 mM for 10 % Intralipid and 2.2 mM for 3 % Intralipid.

  5. Diabetes and puberty : studies on hormonal factors of importance to the blood glucose control

    OpenAIRE

    Zachrisson, Ingmar

    2000-01-01

    It is well known that, in children with type 1 diabetes, metabolic control often deteriorates during puberty. Better understanding of the mechanisms that cause deterioration of blood glucose control can lead to improved therapeutic approaches. Preliminary observations suggest that one component of the impaired glycemic control is a large variation in blood glucose. Several of the hormonal changes during puberty affect insulin sensitivity and could, in addition to lifestyle f...

  6. How to compare adequacy of algorithms to control blood glucose in the intensive care unit?

    OpenAIRE

    Van den Berghe , Greet

    2004-01-01

    Vogelzang et al. retrospectively assessed a derivative marker of blood glucose control over time in the intensive care unit (ICU), "the hyperglycemic index" (HGI), in relation to outcome. The HGI predicted mortality better than other indices of blood glucose control that do not take the duration of hyperglycemia into account. This provided further support to the concept of maintaining normoglycemia with insulin throughout intensive care in order to improve outcome. The HGI was also proposed a...

  7. Clinical implication of blood glucose monitoring in general dental offices: the Ehime Dental Diabetes Study

    OpenAIRE

    Harase, Tadahiro; Nishida, Wataru; Hamakawa, Tomohiro; Hino, Satoshi; Shigematsu, Kenji; Kobayashi, Satoru; Sako, Hirofumi; Ito, Shirou; Murakami, Hajime; Nishida, Kei; Inoue, Hiroshi; Fujisawa, Masahito; Yoshizu, Hiroshi; Kawamura, Ryoichi; Takata, Yasunori

    2015-01-01

    Objective We examined whether general dentists can contribute to the detection of patients with undiagnosed diabetes and prediabetes by monitoring blood glucose in dental clinics. Research design and methods A total of 716 patients who visited clinics for dental treatment were enrolled and classified into 3 groups (mild, moderate, and severe) according to Kornman's criteria for periodontitis. The correlations between the casual blood glucose level, presence or absence of the history of diabet...

  8. Effect of Collaborative Care System (CCS) on Blood Glucose Levels in Type 2 Diabetes Mellitus Outpatient

    OpenAIRE

    Nuryati Kuman; Bangunawati Rahajeng

    2016-01-01

    Some health system in various countries in the world is highly fragmented and is unable to resolve health problems. With collaborative care system, it is expected that DM patients can be managed well. We conducted a study to determine the effect of collaborative care system on blood glucose levels of patients with type 2 diabetes mellitus. This study uses a quasi-experimental design. Data was obtained by measuring random blood glucose levels in patients with type 2 diabetes mellitus. The samp...

  9. Scale Space Methods for Analysis of Type 2 Diabetes Patients' Blood Glucose Values

    OpenAIRE

    Stein Olav Skrøvseth; Fred Godtliebsen

    2011-01-01

    We describe how scale space methods can be used for quantitative analysis of blood glucose concentrations from type 2 diabetes patients. Blood glucose values were recorded voluntarily by the patients over one full year as part of a self-management process, where the time and frequency of the recordings are decided by the patients. This makes a unique dataset in its extent, though with a large variation in reliability of the recordings. Scale space and frequency space techniques are suited to ...

  10. Blood Glucose Alterations in Spinal versus General anesthesia in those undergoing Cesarean Section Delivery

    OpenAIRE

    Alireza Manafi; Habibollah Zakeri; Fatemeh Salahyan; Marzieh Tavassoli; Fahimeh Shekoohi; Roya Kokabi; Sahar Khazforoosh

    2015-01-01

    Introduction: Major body injury or surgery is associated with reproducible metabolic and hormonal responses. Alteration of blood glucose levels is one of the necessary metabolic changes to surgical stress. Surgical techniques and different methods of anesthesia are factors that can help to control and balance the body’s hormones. One of the most effective ways for decline the endocrine-metabolic response is local anesthesia. We conducted this study to compare the measurement of blood glucose ...

  11. System Accuracy Evaluation of the GlucoRx Nexus Voice TD-4280 Blood Glucose Monitoring System

    OpenAIRE

    Muhammad Khan; Keith Broadbent; Mike Morris; David Ewins; Franklin Joseph

    2014-01-01

    Use of blood glucose (BG) meters in the self-monitoring of blood glucose (SMBG) significantly lowers the risk of diabetic complications. With several BG meters now commercially available, the International Organization for Standardization (ISO) ensures that each BG meter conforms to a set degree of accuracy. Although adherence to ISO guidelines is a prerequisite for commercialization in Europe, several BG meters claim to meet the ISO guidelines yet fail to do so on internal validation. We con...

  12. Effect of Sulfonylureas Administered Centrally on the Blood Glucose Level in Immobilization Stress Model

    OpenAIRE

    Sharma, Naveen; Sim, Yun-Beom; Park, Soo-hyun; Lim, Su-Min; Kim, Sung-Su; Jung, Jun-Sub; Hong, Jae-Seung; Suh, Hong-Won

    2015-01-01

    Sulfonylureas are widely used as an antidiabetic drug. In the present study, the effects of sulfonylurea administered supraspinally on immobilization stress-induced blood glucose level were studied in ICR mice. Mice were once enforced into immobilization stress for 30 min and returned to the cage. The blood glucose level was measured 30, 60, and 120 min after immobilization stress initiation. We found that intracerebroventricular (i.c.v.) injection with 30 µg of glyburide, glipizide, glimepir...

  13. Improvements on Noninvasive Blood Glucose Biosensors Using Wavelets for Quick Fault Detection

    OpenAIRE

    Germán Campetelli; David Zumoffen; Marta Basualdo

    2011-01-01

    Noninvasive blood glucose sensors are still under development stage considering that they are far from being suitable for use in anartificial pancreas. The latter has three main parts: the blood glucose sensor, the insulin pump and the controller. However, for the biosensor analyzed here, some common failures such as signal shifts and unreal picks were found. They must be taken into account, for computing the correct insulin dosage for diabetic persons. Hence, a fault det...

  14. The rate-limiting step for glucose transport into the hypothalamus is across the blood-hypothalamus interface

    OpenAIRE

    Poitry-Yamate, Carole; Lei, Hongxia; Gruetter, Rolf

    2009-01-01

    Specialized glucosensing neurons are present in the hypothalamus, some of which neighbor the median eminence, where the blood-brain barrier has been reported leaky. A leaky blood-brain barrier implies high tissue glucose levels and obviates a role for endothelial glucose transporters in the control of hypothalamic glucose concentration, important in understanding the mechanisms of glucose sensing We therefore addressed the question of blood-brain barrier integrity at the hypothalamus for gluc...

  15. The Effect of Tinospora crispa on Serum Glucose and Insulin Levels in Patients with Type 2 Diabetes Mellitus

    OpenAIRE

    Chulaporn Roongpisuthipong; Theerawut Klangjareonchai

    2012-01-01

    Objective. To determine the effects of Tinospora crispa on serum glucose and insulin levels in healthy subjects and patients with type 2 diabetes mellitus. Method. Serum from 10 healthy subjects and 10 diabetic participants, who had fasted overnight, were obtained every 30–60 minutes during the 3 hours of continued fasting and during the 3 hours after ingestion of 75 g of glucose with or without ingestion of 125 or 250 g of Tinospora crispa dry powder capsule. Glucose and Insulin levels were ...

  16. Insoluble Fiber in Young Barley Leaf Suppresses the Increment of Postprandial Blood Glucose Level by Increasing the Digesta Viscosity

    Directory of Open Access Journals (Sweden)

    Akira Takano

    2013-01-01

    Full Text Available Barley (Hordeum vulgare L. is a well-known cereal plant. Young barley leaf is consumed as a popular green-colored drink, which is named “Aojiru” in Japan. We examined the effects of barley leaf powder (BLP and insoluble fibers derived from BLP on postprandial blood glucose in rats and healthy Japanese volunteers. BLP and insoluble fibers derived from BLP suppressed the increment of postprandial blood glucose levels in rats (, and increased the viscosity of their digesta. The insoluble fibers present in BLP might play a role in controlling blood glucose level by increasing digesta viscosity. In human, BLP suppressed the increment of postprandial blood glucose level only in those which exhibited higher blood glucose levels after meals (. BLP might suppress the increment of postprandial blood glucose level by increasing digesta viscosity in both of rats and humans who require blood glucose monitoring.

  17. THE EFFECT OF AVOCADO (PERSEA AMERICANA MILL. LEAVES EXTRACT TOWARDS THE MOUSE'S BLOOD GLUCOSE DECREASE WITH THE GLUCOSE TOLERANCE METHOD

    Directory of Open Access Journals (Sweden)

    Shirly Kumala*, Hesty Utami and Wahyu Kartika Sari

    2013-02-01

    Full Text Available Decrease in blood glucose level test with the use of oral glucose tolerance method from Avocado leaves was carried out. Swiss Webster male mice were divided randomly into five groups. They were negative control, positive control (Glipizide 0.013 mg/20 g BW and three test groups treated with dosage of avocado leaves ethanol extract 0.490 g/kg, 0.980 g/kg and 1.960 g /BW respectively. Experiment was begun with feeding the mice with the test solution followed by feeding glucose solution (1.5 g/kg BW 30 minutes later. Blood glucose levels were assessed using glucometer kit, from zero to 3 hours, at ½ hourly interval. These results analysed by one way ANOVA showed there were significant difference (p<0.05 between Ethanol extract of avocado leaves treated with mice (0.490, 0.980 g/kg BW and control groups. Furthermore, when Tukey’s test was performed, avocado treated mice (1.960g/kg BW reduce glucose level to 64.27%. The effectiveness of this treatment was not significantly different to those treated with glipizide (68.50%.

  18. Blood glucose regulation mechanism in depressive disorder animal model during hyperglycemic states.

    Science.gov (United States)

    Lim, Su-Min; Park, Soo-Hyun; Sharma, Naveen; Kim, Sung-Su; Lee, Jae-Ryeong; Jung, Jun-Sub; Suh, Hong-Won

    2016-06-01

    Depression is more common among diabetes people than in the general population. In the present study, blood glucose change in depression animal model was characterized by various types of hyperglycemia models such as d-glucose-fed-, immobilization stress-, and drug-induced hyperglycemia models. First, the ICR mice were enforced into chronic restraint stress for 2h daily for 2 weeks to produce depression animal model. The animals were fed with d-glucose (2g/kg), forced into restraint stress for 30min, or administered with clonidine (5μg/5μl) supraspinally or spinally to produce hyperglycemia. The blood glucose level in depression group was down-regulated compared to that observed in the normal group in d-glucose-fed-, restraint stress-, and clonidine-induced hyperglycemia models. The up-regulated corticosterone level induced by d-glucose feeding or restraint stress was reduced in the depression group while the up-regulation of plasma corticosterone level is further elevated after i.t. or i.c.v. clonidine administration in the depression group. The up-regulated insulin level induced by d-glucose feeding or restraint stress was reduced in the depression group. On the other hand, blood corticosterone level in depression group was up-regulated compared to the normal group after i.t. or i.c.v. clonidine administration. Whereas the insulin level in depression group was not altered when mice were administered clonidine i.t. or i.c.v. Our results suggest that the blood glucose level in depression group is down-regulated compared to the normal group during d-glucose-fed-, immobilization stress-, and clonidine-induced hyperglycemia in mice. The down-regulation of the blood glucose level might be one of the important pathophysiologic changes in depression. PMID:27034116

  19. Serum Potassium and Glucose Regulation in the ADDITION-Leicester Screening Study

    Science.gov (United States)

    Carter, Patrice; Bodicoat, Danielle H.; Quinn, Lauren M.; Zaccardi, Francesco; Webb, David R.; Khunti, Kamlesh; Davies, Melanie J.

    2015-01-01

    Introduction. Previous observational studies have shown conflicting results between plasma K+ concentrations and risk of type 2 diabetes. To help clarify the evidence we aimed to determine whether an association existed between serum K+ and glucose regulation within a UK multiethnic population. Methods. Participants were recruited as part of the ADDITION Leicester study, a population based screening study. Individuals from primary care between the age of 40 and 75 years if White European or 25 and 75 years if South Asian or Afro Caribbean were recruited. Tests for associations between baseline characteristics and K+ quartiles were conducted using linear regression models. Results. Data showed individuals in the lowest K+ quartile had significantly greater 2-hour glucose levels (0.53 mmol/L, 95% CI: 0.36 to 0.70, P ≤ 0.001) than those in the highest K+ quartile. This estimation did not change with adjustment for potential confounders. Conversely, participants in the lowest K+ quartile had a 0.14% lower HbA1c (95% CI −0.19 to −0.10: P ≤ 0.001) compared to those in the highest K+ quartile. Conclusion. This cross-sectional analysis demonstrated that lower K+ was associated with greater 2 hr glucose. The data supports the possibility that K+ may influence glucose regulation and further research is warranted. PMID:25883988

  20. STUDY OF ERYTHROCYTE SEDIMENTATION RATE,SERUM TOTAL PROTEIN, SERUM ALBUMIN,SERUM GLOBULIN AND RED BLOOD CELL COUNT IN DIFFERENT PHASES OF MENSTRUAL CYCLE

    OpenAIRE

    Shilpa,; Girish M; Patil, MR; Malipatil

    2014-01-01

    : BACKGROUND: Menstruation is a phenomenon unique to females and nearly universal experience in women's lives and is poorly understood. It is characterized by co-ordinate sequence of hormonal changes but the changes that occur in the distribution of hematological parameters, biochemical parameters have not been clearly established. AIMS: To compare Erythrocyte Sedimentation Rate (ESR), Serum total protein, Serum albumin, Serum globulin and Red Blood Cell count (RBC) in dif...

  1. Alanine aminotransferase is associated with an adverse nocturnal blood glucose profile in individuals with normal glucose regulation.

    Directory of Open Access Journals (Sweden)

    Jian Zhou

    Full Text Available OBJECTIVE: Although the association between alanine aminotransferase (ALT levels and risk of type 2 diabetes is well-studied, the effects of slightly increased ALT levels within the normal range on the temporal normal glucose profile remains poorly understood. METHODS: A total of 322 Chinese subjects without impaired glucose tolerance or previous diagnoses of diabetes were recruited for study from 10 hospitals in urban areas across China. All subjects wore a continuous glucose monitoring (CGM system for three consecutive days. The diurnal (06∶00-20∶00 and nocturnal (20∶00-06∶00 mean blood glucose (MBG levels were calculated. Subjects were stratified by ALT quartile level and correlation analyses were performed. RESULTS: The median ALT level was 17 IU/L, and subjects with ALT ≥17 IU/L had higher nocturnal MBG level than those with ALT 0.05. Multivariate stepwise regression analysis of elevated nocturnal MBG identified increased HOMA-IR, elevated ALT levels, and decreased homeostatic model assessment of ß-cell function as independent factors (all, P<0.05. CONCLUSIONS: Mildly elevated ALT levels, within the normal range, are associated with unfavorable nocturnal glucose profiles in Chinese subjects with normal glucose regulation.

  2. Alanine Aminotransferase Is Associated with an Adverse Nocturnal Blood Glucose Profile in Individuals with Normal Glucose Regulation

    Science.gov (United States)

    Li, Hong; Ran, Xingwu; Yang, Wenying; Li, Qiang; Peng, Yongde; Li, Yanbing; Gao, Xin; Luan, Xiaojun; Wang, Weiqing; Jia, Weiping

    2013-01-01

    Objective Although the association between alanine aminotransferase (ALT) levels and risk of type 2 diabetes is well-studied, the effects of slightly increased ALT levels within the normal range on the temporal normal glucose profile remains poorly understood. Methods A total of 322 Chinese subjects without impaired glucose tolerance or previous diagnoses of diabetes were recruited for study from 10 hospitals in urban areas across China. All subjects wore a continuous glucose monitoring (CGM) system for three consecutive days. The diurnal (06∶00–20∶00) and nocturnal (20∶00–06∶00) mean blood glucose (MBG) levels were calculated. Subjects were stratified by ALT quartile level and correlation analyses were performed. Results The median ALT level was 17 IU/L, and subjects with ALT ≥17 IU/L had higher nocturnal MBG level than those with ALT 0.05). Multivariate stepwise regression analysis of elevated nocturnal MBG identified increased HOMA-IR, elevated ALT levels, and decreased homeostatic model assessment of ß-cell function as independent factors (all, P<0.05). Conclusions Mildly elevated ALT levels, within the normal range, are associated with unfavorable nocturnal glucose profiles in Chinese subjects with normal glucose regulation. PMID:23424646

  3. [Effect of raw and cooked nopal (Opuntia ficus indica) ingestion on growth and profile of total cholesterol, lipoproteins, and blood glucose in rats].

    Science.gov (United States)

    Cárdenas Medellín, M L; Serna Saldívar, S O; Velazco de la Garza, J

    1998-12-01

    Two different concentrations (approx. 6 and 12%) and two presentations (raw and cooked) of dehydrated nopal were fed to laboratory rats and growth and serum total cholesterol, lipoprotein profile and glucose determined. Samples of raw and cooked nopal were chemically characterized for moisture, protein, ash, crude fiber, ether extract, total dietary fiber, reducing sugars, amino acids, minerals and gross energy. Cooking slightly affected some of the nutrients analyzed. After one month feeding, blood was withdrawn via intracardiac puncture and serum glucose, total cholesterol, HDL, LDL, and VLDL were determined. Rats fed 12% nopal had lower weight gains (P nopal or the control diet. Consumption of nopal did not affect (P > 0.05) glucose, total cholesterol and HDL cholesterol levels. However, rats fed raw nopal at the 12% concentration level had a 34% reduction in LDL cholesterol levels; thus, it was concluded that raw nopal had a potentially beneficial effect for hypercholesterolemic individuals. PMID:10347696

  4. A vitamin, mineral, herb dietary supplement effect on blood glucose in uncontrolled type II diabetic subjects.

    Science.gov (United States)

    González, Michael J; Ricart, Carlos M; Miranda-Massari, Jorge

    2004-06-01

    We tested a dietary supplement formulated with a synergistic combination of vitamins, minerals, herbals in a group of 15 patients with uncontrolled diabetes type II. The supplement was given for 30 days. Fasting blood glucose was measured prior to the supplementation and at the end of the 30 days treatment period. Blood glucose was significantly reduced in all patients with no adverse effects. This orthomolecular correction of faulty glucose metabolism with a combination of nontoxic, safe and fairly inexpensive nutraceuticals needs to be further substantiated. Nervertheless the idea of correcting metabolism with micronutrients is a new concept of genetic nutritioneering that seems appealing and cost effective. PMID:15377060

  5. Using meta-differential evolution to enhance a calculation of a continuous blood glucose level.

    Science.gov (United States)

    Koutny, Tomas

    2016-09-01

    We developed a new model of glucose dynamics. The model calculates blood glucose level as a function of transcapillary glucose transport. In previous studies, we validated the model with animal experiments. We used analytical method to determine model parameters. In this study, we validate the model with subjects with type 1 diabetes. In addition, we combine the analytic method with meta-differential evolution. To validate the model with human patients, we obtained a data set of type 1 diabetes study that was coordinated by Jaeb Center for Health Research. We calculated a continuous blood glucose level from continuously measured interstitial fluid glucose level. We used 6 different scenarios to ensure robust validation of the calculation. Over 96% of calculated blood glucose levels fit A+B zones of the Clarke Error Grid. No data set required any correction of model parameters during the time course of measuring. We successfully verified the possibility of calculating a continuous blood glucose level of subjects with type 1 diabetes. This study signals a successful transition of our research from an animal experiment to a human patient. Researchers can test our model with their data on-line at https://diabetes.zcu.cz. PMID:27393799

  6. Energized by love: thinking about romantic relationships increases positive affect and blood glucose levels.

    Science.gov (United States)

    Stanton, Sarah C E; Campbell, Lorne; Loving, Timothy J

    2014-10-01

    We assessed the impact of thinking of a current romantic partner on acute blood glucose responses and positive affect over a short period of time. Participants in romantic relationships were randomly assigned to reflect on their partner, an opposite-sex friend, or their morning routine. Blood glucose levels were assessed prior to reflection, as well as at 10 and 25 min postreflection. Results revealed that individuals in the routine and friend conditions exhibited a decline in glucose over time, whereas individuals in the partner condition did not exhibit this decline (rather, a slight increase) in glucose over time. Reported positive affect following reflection was positively associated with increases in glucose, but only for individuals who reflected on their partner, suggesting this physiological response reflects eustress. These findings add to the literature on eustress in relationships and have implications for relationship processes. PMID:24924647

  7. Control of Blood Glucose for People with Type 1 Diabetes: an in Vivo Study

    DEFF Research Database (Denmark)

    Boiroux, Dimitri; Schmidt, Signe; Duun-Henriksen, Anne Katrine;

    2012-01-01

    Since continuous glucose monitoring (CGM) technology and insulin pumps have improved recent years, a strong interest in a closed-loop articial pancreas for people with type 1 diabetes has arisen. Presently, a fully automated controller of blood glucose must face many challenges, such as daily...... variations of patient's physiology and lack of accuracy of glucose sensors. In this paper we design and discuss an algorithm for overnight closed-loop control of blood glucose in people with type 1 diabetes. The algorithm is based on Model Predictive Control (MPC). We use an oset-free autoregressive model...... with exogenous input and moving average (ARMAX) to model the patient. Observer design and a time-varying glucose reference signal improve robustness of the algorithm. We test the algorithm in two clinical studies conducted at Hvidovre Hospital. The rst study took place overnight, and the second one...

  8. Association between whole blood mercury and glucose intolerance among adult Inuit in Greenland

    DEFF Research Database (Denmark)

    Jeppesen, Charlotte; Valera, Beatriz; Nielsen, Nina O; Bjerregaard, Peter; Jørgensen, Marit E

    OBJECTIVES: The Arctic diet is partly constituted by traditional food characterized by top predator animals such as whales, walrus, and seals with high mercury content. Mercury exposure has been associated with glucose intolerance in Western populations. We studied the association between whole...... blood mercury and glucose intolerance in a highly exposed non-Western population METHODS: Cross-sectional study of 2640 Inuit (18+ years) with information on ancestry, smoking, waist circumference, total energy intake, and physical activity. Mercury, fasting- and 2-h plasma glucose, insulin, and c......-peptide were measured in blood. Fasting participants without diabetes were classified into normal glucose tolerance, impaired glucose tolerance, impaired fasting glycemia, or type 2 diabetes. We calculated hepatic insulin resistance with homoeostatic model assessment - insulin resistance index, peripheral...

  9. Photoacoustic blood glucose and skin measurement based on optical scattering effect

    Science.gov (United States)

    Zhao, Zuomin; Myllyla, Risto A.

    2002-07-01

    Non-invasive blood glucose determination has been investigated by more than 100 research groups in the world during the past fifteen years. The commonly optical methods are based on the capacity of near-IR light to penetrate a few hundreds micrometers or a few millimeters into human tissue where it interacts with glucose. A change of glucose concentration may modify the optical parameters in tissue, with the result that its glucose concentration can be extracted by analyzing the received optical signals. This paper demonstrates that glucose affects on the scattering coefficient of human blood, by applying the streak camera and pulsed photoacoustic techniques; and drinking water seems also affecting on PA signal from skin surface.

  10. Development of portable health monitoring system for automatic self-blood glucose measurement

    Science.gov (United States)

    Kim, Huijun; Mizuno, Yoshihumi; Nakamachi, Eiji; Morita, Yusuke

    2010-02-01

    In this study, a new HMS (Health Monitoring System) device is developed for diabetic patient. This device mainly consists of I) 3D blood vessel searching unit and II) automatic blood glucose measurement (ABGM) unit. This device has features such as 1)3D blood vessel location search 2) laptop type, 3) puncturing a blood vessel by using a minimally invasive micro-needle, 4) very little blood sampling (10μl), and 5) automatic blood extraction and blood glucose measurement. In this study, ABGM unit is described in detail. It employs a syringe type's blood extraction mechanism because of its high accuracy. And it consists of the syringe component and the driving component. The syringe component consists of a syringe itself, a piston, a magnet, a ratchet and a micro-needle whose inner diameter is about 80μm. And the syringe component is disposable. The driving component consists of body parts, a linear stepping motor, a glucose enzyme sensor and a slider for accurate positioning control. The driving component has the all-in-one mechanism with a glucose enzyme sensor for compact size and stable blood transfer. On designing, required thrust force to drive the slider is designed to be greater than the value of the blood extraction force. Further, only one linear stepping motor is employed for blood extraction and transportation processes. The experimental result showed more than 80% of volume ratio under the piston speed 2.4mm/s. Further, the blood glucose was measured successfully by using the prototype unit. Finally, the availability of our ABGM unit was confirmed.

  11. Blood flow is an important determinant of forearm glucose uptake following a mixed meal.

    Science.gov (United States)

    Fugmann, A; Sarabi, M; Karlström, B; Berne, C; Lithell, H; Lind, L

    2003-09-01

    Insulin-mediated vasodilation has been suggested to be of importance for glucose uptake during normoglycemic hyperinsulinemia. If this also is valid after an ordinary mixed meal remains to be evaluated. Forearm blood flow (FBF) and forearm glucose uptake change (evaluated by venous occlusion plethysmography) and glucose arteriovenous differences were evaluated over 120 minutes in 10 healthy volunteers following an ordinary mixed meal (700-900 kcal, 34% of energy from fat). Fasting arterial glucose level was 4.9+/-0.9 mmol/l, and the maximum glucose level was reached 30 minutes after the start of ingestion (6.6+/-0.8 mmol/l, p<0.0001). Plasma insulin levels were increased four-fold. FBF increased rapidly within 20 minutes after the start of ingestion and reached its maximum after 50 minutes (94% higher than baseline level, p<0.01). After 2 hours FBF was still substantially elevated (75% above baseline level, p<0.01). Forearm glucose uptake increased fivefold already after 20 minutes ( p<0.01). During the 2 hours, the increase in FBF contributed to 41% of the forearm glucose uptake ( p<0.05). The present study showed that the increase in FBF seen after an ordinary mixed meal is important for the change in forearm glucose uptake. These results support the view that modulation of limb blood flow is a determinant of glucose uptake. PMID:14605966

  12. Stochastic and Optimization Programming for Blood Glucose & Insulin Regulatory Systems of Type-2 Diabetes

    Directory of Open Access Journals (Sweden)

    P. Tirupathi Rao

    2012-11-01

    Full Text Available In this paper a stochastic modeling and optimization problem is developed for blood glucose and insulin regulatory system suitable for management of type-2 diabetes patients. The rates of arrival and consumption of glucose and insulin in the blood are assumed as Poisson parameters and developed a model through stochastic processes. A stochastic programming problem for maintaining the optimized glucose and insulin levels of the blood is developed. An objective function for maximizing the energy release subject to the constraints on the consumption of glucose is formulated. Sensitivity analysis was carried out and observed the model behaviour through influencing parameters. Enhancing the developed programming problem as desk top automation system will make this work more accessible to the decision makers of Health care industry.

  13. Evaluation of two methods of rapid blood-glucose monitoring by unskilled personnel during surgery

    DEFF Research Database (Denmark)

    Madsbad, S; Adelhøj, B; Bigler, Dennis Richard;

    1984-01-01

    % of the readings below laboratory values -20%. All situations with severe hypoglycaemia were detected with both strips. The study also demonstrates the ineffectiveness of s.c. insulin regimens during surgery. Only 47% of the measured blood glucose values were within the range of 5.5-10 mmol/l and two......The accuracy of two rapid methods of blood-glucose monitoring without (Haemo-glucotest 1-44) and with a reflectance meter (Hypocount B) was compared using a laboratory method. The assessment was carried out by personnel with no previous experience in measuring blood glucose. Eighty-five percent of...... the 92 measurements obtained with the hypocount B were within +/- 20% of the laboratory glucose values. Using haemo-glucotest 1-44 strips, 74% of the readings were within +/- 20% of the reference laboratory values. For values below 5.5 mmol/l, there was a tendency for results to be too low, with 77...

  14. EVALUATION OF THE EFFECT OF “WAKOUBA '' ON THE LIPID PROFILE, SYSTOLIC BLOOD PRESSURE (SBP DIASTOLIC (DBP AND BLOOD GLUCOSE IN HYPERTENSIVE RABBITS

    Directory of Open Access Journals (Sweden)

    Tiekpa Wawa J

    2014-11-01

    Full Text Available This study aims at evaluating the effects of Wakouba, an extract of the fronds of oil palm tree Elaeis guineensis (Jacq traditionally used in the treatment of high blood pressure, on lipid profile, urea, creatinine, blood glucose, systolic blood pressure (SBP, diastolic (DBP and heart rate (HR in hypertensive rabbits. Methods: Twenty four rabbits (24 divided into six (06 groups of four rabbits each weighing between 1.5 ±3.0 to 2± 1.5 kg were used. Group 1 served as witness, group 2 sick and untreated control, groups 3, 4, 5, and 6 were used as experimental groups (sick+treatment. Throughout the experiment the witness group received distilled water; adrenaline was administered to the sick control group. After 10 days of adrenaline injection, hypertension has been stabilized in sick groups (group 2 to 6 and blood was taken for the determination of urea, creatinine, and lipids. Four (04 of the five (05 groups of hypertensive rabbits were treated with two (2 doses of " Wakouba " 950 and 2500 mg / kg bw and two ( 02 doses of ténordate 10 and 20 mg / kg BW, two(2 group by two(2 group . After ten (10 days of treatment, the SBP and DBP and HR were measured, blood was collected for determination of the same biochemical parameters. Results: The study of the effect of Wakouba and ternodate on the changes in systolic blood pressure SBP, DBP and heart rate (HR showed a significant decrease (P up to normalization of these parameters after 10 days of treatment. Similarly, the measurement of serum lipid profile in hypertensive rabbits treated with Wakouba (950mg / kg bw and tenordate (20 mg / kg bw showed a significant reduction in (P values of total cholesterol, LDL cholesterol and triglycerides in contrast to HDL cholesterol which has increased significantly compared to the control group. Same doses also normalize serum glucose, urea and creatinine. Histological sections performed on the kidney and the heart of hypertensive rabbit showed congestion of

  15. Measurement of tissue optical properties with optical coherence tomography: Implication for noninvasive blood glucose concentration monitoring

    Science.gov (United States)

    Larin, Kirill V.

    Approximately 14 million people in the USA and more than 140 million people worldwide suffer from diabetes mellitus. The current glucose sensing technique involves a finger puncture several times a day to obtain a droplet of blood for analysis. There have been enormous efforts by many scientific groups and companies to quantify glucose concentration noninvasively using different optical techniques. However, these techniques face limitations associated with low sensitivity, accuracy, and insufficient specificity of glucose concentrations over a physiological range. Optical coherence tomography (OCT), a new technology, is being applied for noninvasive imaging in tissues with high resolution. OCT utilizes sensitive detection of photons coherently scattered from tissue. The high resolution of this technique allows for exceptionally accurate measurement of tissue scattering from a specific layer of skin compared with other optical techniques and, therefore, may provide noninvasive and continuous monitoring of blood glucose concentration with high accuracy. In this dissertation work I experimentally and theoretically investigate feasibility of noninvasive, real-time, sensitive, and specific monitoring of blood glucose concentration using an OCT-based biosensor. The studies were performed in scattering media with stable optical properties (aqueous suspensions of polystyrene microspheres and milk), animals (New Zealand white rabbits and Yucatan micropigs), and normal subjects (during oral glucose tolerance tests). The results of these studies demonstrated: (1) capability of the OCT technique to detect changes in scattering coefficient with the accuracy of about 1.5%; (2) a sharp and linear decrease of the OCT signal slope in the dermis with the increase of blood glucose concentration; (3) the change in the OCT signal slope measured during bolus glucose injection experiments (characterized by a sharp increase of blood glucose concentration) is higher than that measured in

  16. Ratio of blood serum ferritin and ferritin-bearing lymphocytes in breast cancer

    International Nuclear Information System (INIS)

    The ratio of the blood serum ferritin level and the amount of ferritin-bearing lymphocytes was determined in breast cancer patients and mastopathy patients. Direct relation between a rise of blood serum ferritin concentration, a drop in the amount of ferritin-bearing cells and tumor spreading was established in breast cancer. The maximum amount of ferritin-bearing cells was present in the blood of the patients with Stage I-IIA disease. Radical mastectomy in such patients returned the blood serum ferritin level and the amount of ferritin-bearing cells to normal

  17. Association of fasting plasma glucose and serum lipids in Type 2 diabetics

    Directory of Open Access Journals (Sweden)

    S Khadke

    2015-01-01

    Full Text Available Dyslipidemia is a significant morbidity associated with diabetes and cardiovascular disorders. The present study was undertaken to assess the lipid profile of type 2 diabetic and age-gender matched healthy subjects and its association, if any, with fasting plasma glucose. Clinically diagnosed diabetic subjects were recruited for the study. The fasting plasma glucose and lipid profiles were analyzed for 99 diabetic and 101 healthy volunteers. The blood samples were analyzed for fasting plasma glucose, total cholesterol, triglycerides, high density lipoprotein-cholesterol, low density lipoprotein-cholesterol and very low density lipoprotein-cholesterol. Correlation analysis of lipid profile with fasting plasma glucose and calculation of risk ratio was done. The levels of high density lipoprotein-cholesterol and low density lipoprotein-cholesterol were found to be significantly low in diabetics and subjects with lower low density lipoprotein-cholesterol were on statins. Inspite of lower lipid values, the risk ratio for diabetics was significantly higher. The correlation analysis indicated significant difference in relationship between fasting plasma glucose, lipid parameters and risk ratios in the two groups. Diabetics with lower high density lipoprotein-cholesterol and higher total cholesterol present with a higher risk ratio pointing to need of non-statin high density lipoprotein-raising medications decreasing their predisposition to cardiovascular disorders. The study highlights the altered pattern of correlation of lipid profile with fasting plasma glucose in diabetics and their increased risk of cardiovascular disorders. The dyslipidemia in the form of triglyceridemia and significantly low high density lipoprotein-cholesterol in diabetics point towards the need of non-statin high density lipoprotein-raising medications.

  18. Continuous monitoring of blood glucose in the practice of endocrinologist

    Directory of Open Access Journals (Sweden)

    Yu I Philippov

    2012-12-01

    Full Text Available Continuous glucose monitoring - an important diagnostic, teaching and treatment tool for patients with diabetes mellitus, which is increasingly becoming a part of routine clinical practice in endocrinology. This article presents an overview of modern techniques, their advantages and disadvantages, evidence basis and place in everyday clinical practice. The article discusses the key factors affecting the efficiency, indications, contraindications, conditions of use of the continuous glucose monitoring systems in patients with diabetes mellitus, gives an algorithm for the application of this technique in clinical practice.

  19. Self-Blood Glucose Monitoring in Diabetes Mellitus (Beyond the Basics)

    Science.gov (United States)

    ... Use ©2016 UpToDate, Inc. Patient education: Self-blood glucose monitoring in diabetes mellitus (Beyond the Basics) Author ... 2016. | This topic last updated: Oct 19, 2015. BLOOD SUGAR TESTING OVERVIEW — If you have diabetes, you have ...

  20. Quality assessment of patients’ self-monitoring of blood glucose in community pharmacies

    Directory of Open Access Journals (Sweden)

    Kjome RL

    2010-03-01

    Full Text Available Objective: To evaluate diabetes patients’ self-monitoring of blood glucose using a community pharmacy-based quality assurance procedure, to investigate whether the procedure improved the quality of the patient performance of self monitoring of blood glucose, and to examine the opinions of the patients taking part in the study. Methods: The results of patient blood glucose measurements were compared to the results obtained with HemoCue Glucose 201+ by pharmacy employees in 16 Norwegian community pharmacies. Patient performance was monitored using an eight item checklist. Patients whose blood glucose measurements differed from pharmacy measurements by more than 20% were instructed in the correct use of their glucometer. The patients then re-measured their blood glucose. If the results were still outside the set limits, the control procedure was repeated with a new lot of glucometer strips, and then with a new glucometer. The patients returned for a follow-up visit after three months. Results: During the first visit, 5% of the 338 patients had measurements that deviated from pharmacy blood glucose values by more than 20% and user errors were observed for 50% of the patients. At the second visit, there was no significant change in the analytical quality of patient measurements, but the percentage of patients who made user errors had decreased to 29% (p < 0.001. Eighty-five percent of the patients reported that they used their blood glucose results to adjust medication, exercise or meals. Fifty-one percent of the patients reported a greater trust in their measurements after the second visit. Eighty percent of patients wished to have their measurements assessed yearly. Of these patients, 83% preferred to have the assessment done at the community pharmacy. Conclusion: A community pharmacy-based quality assessment procedure of patients’ self monitoring of blood glucose significantly reduced the number of user errors. The analytical quality of the

  1. Correlation of salivary glucose, blood glucose and oral candidal carriage in the saliva of type 2 diabetics: A case-control study

    OpenAIRE

    Satish Kumar; Padmashree, S.; Rema Jayalekshmi

    2014-01-01

    Objectives: To study the correlation between blood glucose levels and salivary glucose levels in type 2 diabetic patients, to study the relationship between salivary glucose levels and oral candidal carriage in type 2 diabetic patients and to determine whether salivary glucose levels could be used as a noninvasive tool for the measurement of glycemic control in type 2 diabetics. Study Design: The study population consisted of three groups: Group 1 consisted of 30 controlled diabetics and Grou...

  2. A soluble activin receptor type IIB does not improve blood glucose in streptozotocin-treated mice.

    Science.gov (United States)

    Wang, Qian; Guo, Tingqing; Portas, Jennifer; McPherron, Alexandra C

    2015-01-01

    Type 1 diabetes mellitus (T1DM), or insulin dependent DM, is accompanied by decreased muscle mass. The growth factor myostatin (MSTN) is a negative regulator of muscle growth, and a loss of MSTN signaling has been shown to increase muscle mass and prevent the development of obesity, insulin resistance and lipodystrophic diabetes in mice. The effects of MSTN inhibition in a T1DM model on muscle mass and blood glucose are unknown. We asked whether MSTN inhibition would increase muscle mass and decrease hyperglycemia in mice treated with streptozotocin (STZ) to destroy pancreatic beta cells. After diabetes developed, mice were treated with a soluble MSTN/activin receptor fused to Fc (ACVR2B:Fc). ACVR2B:Fc increased body weight and muscle mass compared to vehicle treated mice. Unexpectedly, ACVR2B:Fc reproducibly exacerbated hyperglycemia within approximately one week of administration. ACVR2B:Fc treatment also elevated serum levels of the glucocorticoid corticosterone. These results suggest that although MSTN/activin inhibitors increased muscle mass, they may be counterproductive in improving health in patients with T1DM. PMID:25561902

  3. STUDY OF ERYTHROCYTE SEDIMENTATION RATE,SERUM TOTAL PROTEIN, SERUM ALBUMIN,SERUM GLOBULIN AND RED BLOOD CELL COUNT IN DIFFERENT PHASES OF MENSTRUAL CYCLE

    Directory of Open Access Journals (Sweden)

    Shilpa

    2014-09-01

    Full Text Available : BACKGROUND: Menstruation is a phenomenon unique to females and nearly universal experience in women's lives and is poorly understood. It is characterized by co-ordinate sequence of hormonal changes but the changes that occur in the distribution of hematological parameters, biochemical parameters have not been clearly established. AIMS: To compare Erythrocyte Sedimentation Rate (ESR, Serum total protein, Serum albumin, Serum globulin and Red Blood Cell count (RBC in different phases of menstrual cycle. MATERIALS & METHODS: The present study was carried out on 100 healthy female medical students in the age group of 18 to 23years with normal menstrual cycle. E.S.R, Serum total protein, Serum albumin, Serum globulin and RBC count were studied on 2nd day MP(Menstrual Phase,11th day PP(Proliferative Phase, 22ndday SP(Secretory Phase of menstrual cycle using Wintergreen’s method, semi auto analyzer Erba chem-7(BCG Dye method for serum proteins, Hemo Auto analyzer SYSMEX KX-21 respectively. Statistical analysis was done using SPSS 17.0 Software. To compare means of two independent groups, student’s t- test for independent samples was used. RESULTS: The study revealed that ESR was significantly (P<0.01 higher in menstrual phase compared to proliferative phase. Serum total protein was significantly increased in Secretory Phase (SP compared to Menstrual Phase (MP (P<0.05. Serum albumin was significantly (P<0.05 increased in proliferative phase compared to secretory phase and menstrual phase. Serum globulin was significantly increased in SP compared to PP (P<0.05. Red blood cell count showed no significant changes during various phases of menstrual cycle. CONCLUSION: In the present study we found significantly low level of serum albumin and decreased RBC count though not significant increases rouleaux formation thus increasing ESR in the menstrual phase but serum globulin and serum total protein showed increase in secretory phase which does not

  4. Tunable laser diode system for noninvasive blood glucose measurements.

    Science.gov (United States)

    Olesberg, Jonathon T; Arnold, Mark A; Mermelstein, Carmen; Schmitz, Johannes; Wagner, Joachim

    2005-12-01

    Optical sensing of glucose would allow more frequent monitoring and tighter glucose control for people with diabetes. The key to a successful optical noninvasive measurement of glucose is the collection of an optical spectrum with a very high signal-to-noise ratio in a spectral region with significant glucose absorption. Unfortunately, the optical throughput of skin is low due to absorption and scattering. To overcome these difficulties, we have developed a high-brightness tunable laser system for measurements in the 2.0-2.5 microm wavelength range. The system is based on a 2.3 microm wavelength, strained quantum-well laser diode incorporating GaInAsSb wells and AlGaAsSb barrier and cladding layers. Wavelength control is provided by coupling the laser diode to an external cavity that includes an acousto-optic tunable filter. Tuning ranges of greater than 110 nm have been obtained. Because the tunable filter has no moving parts, scans can be completed very quickly, typically in less than 10 ms. We describe the performance of the present laser system and avenues for extending the tuning range beyond 400 nm. PMID:16390586

  5. Effects of aerobic exercising combined with glutamine supplement on the serum GLP-1, insulin and blood glucose levels of rats with T2DM%有氧运动联合谷氨酰胺补充对T2DM大鼠血清GLP-1、胰岛素及血糖水平的影响

    Institute of Scientific and Technical Information of China (English)

    付德荣; 刘承宜; 李鹏博; 田祯祥; 孙小华; 廖八根

    2012-01-01

      In order to observe the effects of aerobic exercising combined with glutamine (Gln) supplement on the serum glucagon like peptide 1 (GLP-1), insulin (INS) and fasting blood glucose (FBG) levels of rats with type 2 diabetes mellitus (T2DM), the authors divided 60 male SD rats ((179.8±19.2) g) randomly into a healthy control group (group C, 26 rats) and a diabetic model establishment ground (group D, 34 rats). The rats in group C were fed with common feed, while the rats in group D were fed with high fat feed. 4 weeks later, the rats in group D were in-jected with 35 mg/kg of streptozotocin (STZ) to induce T2DM. After model formation, the rats in the two groups were further divided into calm groups (CQ, DQ), exercising groups (CE, DE), Gln groups (CG, DG) and exercising plus Gln groups (CEG, DEG) randomly. The rats in the exercising groups did a 6-week swimming exercise. The rats in the Gln groups were fed with Gln a mass fraction of 2%L. Abdominal aorta blood was taken to test FBG, insu-lin and GLP-1 levels. Results:6-week swimming exercise or Gln supplement significantly increased the GLP-1 and insulin concentrations of the rats in group D, significantly decreased FBG value, significantly improved the symptom of polyphagia of the rats in group D, but had no significant effect on the body weight. 6-week exercising significantly increased the insulin level of the rats in group C, significantly decreased their FBG value, had no sig-nificant effect on GLP-1;6-week Gln supplement significantly decreased the food intake of the rats in group C, had no significant effect on their FBG, insulin and GLP-1 levels. When exercising was combined with Gln supplement, the control of FBG, the increase of plasma GLP-1, the increase of insulin level and the improvement of consumption symptom of the rats in group D were more significant than those showed when exercising or Gln supplement worked separately, but there was no significant difference in the effect on the body weight

  6. Study on optical measurement conditions for noninvasive blood glucose sensing

    Science.gov (United States)

    Xu, Kexin; Chen, Wenliang; Jiang, Jingying; Qiu, Qingjun

    2004-05-01

    Utilizing Near-infrared Spectroscopy for non-invasive glucose concentration sensing has been a focusing topic in biomedical optics applications. In this paper study on measuring conditions of spectroscopy on human body is carried out and a series of experiments on glucose concentration sensing are conducted. First, Monte Carlo method is applied to simulate and calculate photons" penetration depth within skin tissues at 1600 nm. The simulation results indicate that applying our designed optical probe, the detected photons can penetrate epidermis of the palm and meet the glucose sensing requirements within the dermis. Second, we analyze the influence of the measured position variations and the contact pressure between the optical fiber probe and the measured position on the measured spectrum during spectroscopic measurement of a human body. And, a measurement conditions reproduction system is introduced to enhance the measurement repeatability. Furthermore, through a series of transmittance experiments on glucose aqueous solutions sensing from simple to complex we found that though some absorption variation information of glucose can be obtained from measurements using NIR spectroscopy, while under the same measuring conditions and with the same modeling method, choices toward measured components reduce when complication degree of components increases, and this causes a decreased prediction accuracy. Finally, OGTT experiments were performed, and a PLS (Partial Least Square) mathematical model for a single experiment was built. We can easily get a prediction expressed as RMSEP (Root Mean Square Error of Prediction) with a value of 0.5-0.8mmol/dl. But the model"s extended application and reliability need more investigation.

  7. CORRELATIONS BETWEEN BLOOD PRESSURE AND BODY WEIGHT, SERUM LEPTIN IN HIGH CALORIE DIET-INDUCED OBESE RATS

    Institute of Scientific and Technical Information of China (English)

    Hu Zhi; Ma Aiqun; Yang Chun; Tian Hongyan

    2006-01-01

    Objective To examine the change of body weight (BW) and blood pressure (BP) in obese rats, clarify relationships between BP and BW and other factors. Methods Male Spraque-Dawley rats were fed either with normal diet (ND) or high calorie diet (HC) for 20 weeks. BW and BP of tail artery were observed biweekly and tetraweekly respectively; serum leptin and fasting insulin (FINS) were detected by enzyme-linked immunoadsordent assay (ELISA) and radioimmunoassay (RIA) respectively. Fasting plasma glucose (FPG) and free fatty acid(FFA) were measured by conventional means. Results BW, abdominal fat weight (AFW), ratio of abdominal fat weight to body weight (RF/W), systolic blood pressure (SBP), diastolic blood pressure (DBP), serum levels of leptin and FINS, FPG, FFA increased in the HD group after 20 weeks diet intervention (P<0.05 or P<0.01). SBP was strongly correlated with BW, leptin, FINS and FFA (P<0.05), DBP was correlated with FFA (r=0.47, P<0.05). In addition, leptin was positively correlated with BW, AFW, RF/W, FINS and FFA (P<0.05 or P<0.01). Conclusion In this study of high calorie-diet induced rats, the gain of BW is accompanied by increased BP. The obese rats have hyperleptinemia, hyperinsulinemia, hyperglycemia and dyslipidemia which may have important effects on the development of obesity-related hypertension. RF/W is the key factor in which affect serum leptin level.

  8. The associations of a marine diet with plasma lipids, blood glucose, blood pressure and obesity among the inuit in Greenland

    DEFF Research Database (Denmark)

    Bjerregaard, P; Pedersen, H S; Mulvad, G

    2000-01-01

    OBJECTIVE: To analyse the associations between the intake of fish and marine mammals and risk factors for cardiovascular disease, ie lipid profile, fasting blood glucose, blood pressure and obesity, in a population whose average consumption of n-3 fatty acids is high compared with Western countries...... statistically significant. The pattern was similar within groups with low, medium and high consumption of marine food. CONCLUSIONS: There are statistically significant associations between the consumption of marine food and certain lipid fractions in the blood also in this population with a very high average...... intake of marine food. The observation that blood glucose is positively associated with marine diet in a population survey is new and should be repeated. There was good agreement between the results for the reported consumption of seal and those for the biomarkers. SPONSORSHIP: The study was financially...

  9. [Effect of a dehydrated extract of nopal (Opuntia ficus indica Mill.) on blood glucose].

    Science.gov (United States)

    Frati-Munari, A C; de León, C; Ariza-Andraca, R; Bañales-Ham, M B; López-Ledesma, R; Lozoya, X

    1989-01-01

    To assess if a dehydrated extract of nopal stems retains the effect on glycemia of the entire nopal stems two experiments were performed. A. Six patients with type II diabetes mellitus in fasting condition received 30 capsules containing 10.1 +/- 0.3 g of the extract, and serum glucose levels were measured hourly from 0 to 180 minutes. B. Six healthy volunteers received 30 capsules with the extract followed by 74 g of dextrose orally. Serum glucose measurements were made in a similar fashion. In each experiment a control test with empty capsules was performed. Nopal extract did not reduce fasting glycemia in diabetic subjects. Nevertheless, the extract diminished the increase of serum glucose which followed a dextrose load. Peak serum glucose was 20.3 +/- 18.2 mg/dl (X +/- SD) lower in the test with nopal than in the control one (P less than 0.025). Dehydrated extract of nopal (Opuntia ficus-indica Mill) did not show acute hypoglycemic effect, although could attenuate postprandial hyperglycemia. PMID:2561256

  10. Changing optical properties of blood serum proteins in case of oncological diseases

    Science.gov (United States)

    Petrova, G. P.; Petrusevich, Yu. M.; Boiko, A. V.; Ivanov, A. V.; Papish, E. A.; Khlapov, V. P.; Fedorova, K. V.

    2007-03-01

    Molecular methods of diagnostics of widespread diseases including oncological pathology on the base static and dynamic laser light scattering in serum blood solution are testified. Rayleigh - Debye laser light scattering method are used to measure molecular parameters of blood serum. Dynamical parameters of macromolecules can be measured by photon correlation spectroscopy method. We obtained that the parameter of intermolecular interaction B for serum blood solution of oncological patients is considerably less then B for serum blood solution of healthy persons and in a number of cases has even a negative value. The effective mass of scattering particles in serum water solutions for samples of oncology diseases patients increase in comparison to control samples. As follows from our experimental results, there is the difference between dynamic molecular parameters for control samples and ones with oncological pathology.

  11. The fluctuation of blood glucose, insulin and glucagon concentrations before and after insulin therapy in type 1 diabetes

    Science.gov (United States)

    Arif, Idam; Nasir, Zulfa

    2015-09-01

    A dynamical-systems model of plasma glucose, insulin and glucagon concentrations has been developed to investigate the effects of insulin therapy on blood glucose, insulin and glucagon regulations in type 1 diabetic patients. Simulation results show that the normal regulation of blood glucose concentration depends on insulin and glucagon concentrations. On type 1 diabetic case, the role of insulin on regulating blood glucose is not optimal because of the destruction of β cells in pancreas. These β cells destructions cause hyperglycemic episode affecting the whole body metabolism. To get over this, type 1 diabetic patients need insulin therapy to control the blood glucose level. This research has been done by using rapid acting insulin (lispro), long-acting insulin (glargine) and the combination between them to know the effects of insulin therapy on blood glucose, insulin and glucagon concentrations. Simulation results show that these different types of insulin have different effects on blood glucose concentration. Insulin therapy using lispro shows better blood glucose control after consumption of meals. Glargin gives better blood glucose control between meals and during sleep. Combination between lispro and glargine shows better glycemic control for whole day blood glucose level.

  12. A novel automated discontinuous venous blood monitoring system for ex vivo glucose determination in humans.

    Science.gov (United States)

    Schaller, R; Feichtner, F; Köhler, H; Bodenlenz, M; Plank, J; Wutte, A; Mader, J K; Ellmerer, M; Hellmich, R; Wedig, H; Hainisch, R; Pieber, T R; Schaupp, L

    2009-03-15

    Intensive insulin therapy reduces mortality and morbidity in critically ill patients but imposes great demands on medical staff who must take frequent blood samples for the determination of glucose levels. A solution to this resourcing problem would be provided by an automated blood monitoring system. The aim of the present clinical study was to evaluate such a system comprising an automatic blood sampling unit linked to a glucose biosensor. Our approach was to determine the correlation and system error of the sampling unit alone and of the combined system with respect to reference levels over 12h in humans. Two venous cannulae were inserted to connect the automatic and reference systems to the subjects. Blood samples were taken at 15 and 30 min intervals. The median Pearson coefficient of correlation between manually and automatically withdrawn blood samples was 0.982 for the sampling unit alone and 0.950 for the complete system. The biosensor had a linear range up to 20 mmoll(-1) and a 95% response time of Titration Error Grid analysis suggested an acceptable treatment in 99.56% of cases. Implementation of a "Keep Vein Open" saline infusion into the automated blood sampling system reduced blood withdrawal failures through occluded catheters fourfold. In summary, automated blood sampling from a peripheral vein coupled with automatic glucose determination is a promising alternative to frequent manual blood sampling. PMID:19135351

  13. Local cerebral blood flow and glucose metabolism during seizure in spontaneously epileptic El mice

    International Nuclear Information System (INIS)

    Local cerebral blood flow and glucose metabolism were examined in spontaneously epileptic El mice using autoradiography with 125I-IMP and 14C-DG in the interictal phase and during seizure. El (+) mice that developed generalized tonic-clonic convulsions and El (-) mice that received no stimulation and had no history of epileptic seizures were examined. The seizure non-susceptible, maternal strain ddY mice were used as control. Uptake ratios for IMP and DG in mouse brain were calculated using the autoradiographic density. In the interictal phase, the pattern of local cerebral blood flow of El (+) mice was similar to that of ddY and El (-) mice, and glucose metabolism in the hippocampus was higher in El (+) mice than in El (-) and ddY mice, but flow and metabolism were nearly matched. During seizure, no significant changed blood flow and increased glucose metabolism in the hippocampus, the epileptic focus, and no markedly changed blood flow and depressed glucose metabolism in other brain regions were observed and considered to be flow-metabolism uncoupling. These observations have never been reported in clinical or experimental studies of epilepsy. Seizures did not cause large regional differences in cerebral blood flow. Therefore, only glucose metabolism is useful for detection of the focus of secondary generalized seizures in El mice, and appeared possibly to be related to the pathophysiology of secondary generalized epilepsy in El mice. (author)

  14. Patient self-monitoring of blood glucose and refinements of conventional insulin treatment.

    Science.gov (United States)

    Tattersall, R; Gale, E

    1981-01-01

    The compelling evidence that blood glucose control will slow or prevent microvascular complications has stimulated research to find better ways of managing insulin-dependent diabetes. The excellent results obtained with "open loop" insulin infusion systems suggest that the relative failure of conventional treatment is the result of (1) a lack of appropriate feedback to the patient and (2) the use of insulin regimens which do not mimic physiologic insulinemia, particularly in the basal state. Doctors regard blood glucose measurements as an essential part of diabetic management and extension of this technology to patients has added a new dimension, particularly in the assessment of control. Nevertheless, home blood-glucose monitoring will not necessarily improve diabetic control; the best results have been obtained when it has been offered as part of a package deal which includes more investment of time and interest by patients and doctor together with joint discussions of problems and changes in treatment. The biggest problem with conventional twice daily insulin regimens is to sustain constant basal insulin levels during the night. Attempts to obtain fasting normoglycemia with an injection before supper often result in nocturnal hyperinsulinemia and hypoglycemia. This can usually be resolved by changing to a three times daily regimen with an extra injection of NPH insulin at bedtime. Three times daily insulin injections with feedback from home blood-glucose monitoring give as good blood glucose control as infusion systems and are cheaper and more acceptable to patients. PMID:7006390

  15. How Effective is Swedish Massage on Blood Glucose Level in Children with Diabetes Mellitus?

    Directory of Open Access Journals (Sweden)

    Firoozeh Sajedi

    2011-09-01

    Full Text Available "nThis study was conducted to determine the effect of Swedish massage on blood glucose level in children with diabetes mellitus (DM. It was prospective randomized controlled trial study that conducted on 36 children, 6-12 years old with DM, recruited from a hospital in Qom City, Iran. The children were randomly assigned to intervention and control groups. Swedish massage was performed 15 minutes, 3 times a week, for 3 months in intervention group. The blood glucose levels were evaluated immediately after every session of massage in two groups. The mean ages of children in the intervention (n=18 and control (n=18 groups were 9.05 ± 1.55 and 9.83 ±2.03 years respectively. There was statistically no significant difference in blood glucose levels before intervention between two groups (P=0.586, but the blood glucose levels were lower significantly in intervention group in comparison with control group after intervention (P<0.0001. Addition of Swedish massage to daily routines; exercise, diet and medication regimens, is an effective intervention to reduce blood glucose level in diabetic children.

  16. Dextrose in the banked blood products does not seem to affect the blood glucose levels in patients undergoing liver transplantation

    Institute of Scientific and Technical Information of China (English)

    Kwok-Wai Cheng; King-Wah Chiu; Shih-Hor Wang; Chih-Che Lin; Tsan-Shiun Lin; Yueh-Wei Liu; Bruno Jawan; Chao-Long Chen; Yu-Fan Cheng; Chia-chih Tseng; Chih-Hsien Wang; Yaw-Sen Chen; Chih-Chi Wang; Tung Liang Huang; Hock-Liew Eng

    2005-01-01

    AIM: Hyperglycemia commonly seen in liver transplantation (LT) has often been attributed to the dextrose in the storage solution of blood transfusion products. The purpose of the study is to compare the changes of the blood glucose levels in transfused and non-transfused patients during LT. METHODS: A retrospective study on 60 biliary pediatric patients and 16 adult patients undergoing LT was carried out. Transfused pediatric patients were included in Group Ⅰ (GI), those not transfused in Group Ⅱ (GⅡ). Twelve adult patients were not given transfusion and assigned to Group Ⅲ (GⅢ); whereas, four adult patients who received massive transfusion were assigned to Group Ⅳ (GⅣ). The blood glucose levels, volume of blood transfused, and the volume of crystalloid infused were recorded, compared and analyzed. RESULTS: Results showed that the changes in bloodglucose levels during LT for both non-transfused and minimally transfused pediatric groups and non-transfused and massively-transfused adult groups were almost the same. CONCLUSION: We conclude that blood transfusion does not cause significant changes in the blood glucose levels inthis study.

  17. Functionalized graphene oxide for clinical glucose biosensing in urine and serum samples

    Directory of Open Access Journals (Sweden)

    Veerapandian M

    2012-12-01

    Full Text Available Murugan Veerapandian,1 Yeong-Tai Seo,2 Hyunkyung Shin,3 Kyusik Yun,1 Min-Ho Lee41Department of Bionanotechnology, Gachon University, Gyeonggi-Do, 2School of Electrical Engineering and Computer Science, Seoul National University, Seoul, 3Department of Mathematics and Information, Gachon University, Gyeonggi-Do, 4Korea Electronics Technology Institute, Medical IT Technology, Gyeonggi-Do, Republic of KoreaAbstract: A novel clinical glucose biosensor fabricated using functionalized metalloid-polymer (silver-silica coated with polyethylene glycol hybrid nanoparticles on the surface of a graphene oxide nanosheet is reported. The cyclic voltammetric response of glucose oxidase modification on the surface of a functionalized graphene oxide electrode showed a surface-confined reaction and an effective redox potential near zero volts, with a wide linearity of 0.1–20 mM and a sensitivity of 7.66 µA mM-1 cm-2. The functionalized graphene oxide electrode showed a better electrocatalytic response toward oxidation of H2O2 and reduction of oxygen. The practical applicability of the functionalized graphene oxide electrode was demonstrated by measuring the peak current against multiple urine and serum samples from diabetic patients. This new hybrid nanoarchitecture combining a three-dimensional metalloid-polymer hybrid and two-dimensional graphene oxide provided a thin solid laminate on the electrode surface. The easy fabrication process and retention of bioactive immobilized enzymes on the functionalized graphene oxide electrode could potentially be extended to detection of other biomolecules, and have broad applications in electrochemical biosensing.Keywords: clinical diagnostics, glucose biosensor, metalloid-polymer nanoparticles, glucose oxidase, cyclic voltammetry

  18. Alterations in blood glucose levels under hyperinsulinemia affect accumbens dopamine

    OpenAIRE

    Bello, Nicholas T.; Hajnal, Andras

    2006-01-01

    Dopaminergic systems have been implicated in diabetes and obesity. Notwithstanding, the most basic relationship between dopamine and plasma insulin as well as glucose levels yet remains unknown. The present experiments were designed to investigate the effects of acute hyperinsulinemia on basal dopamine levels in the nucleus accumbens of the rat under chloral hydrate anesthesia using acute microdialysis in combination with the hyperinsulinemic-glycemic clamping procedure. In Experiment 1, each...

  19. Study on umbilical venous blood serum leptin and plasma neuropeptide Y levels in full term newborns

    International Nuclear Information System (INIS)

    Objective: To investigate the levels and clinical significance of umbilical venous blood serum leptin and plasma neuropeptide Y(NPY) in full term newborns. Methods: Umbilical venous blood serum leptin and plasma NPY levels were measured with radioimmunoassay. Results: The mean level of umbilical venous blood serum leptin was 7.15 +- 3.72 μg/L, the mean level of plasma NPY was 112.07 +- 42.36 ng/L. Levels in female fetal cord blood were significantly higher than those in males (p < 0.01). There were significantly positive correlations between cord venous blood serum leptin and birth weight of newborn and maternal body mass index (BMI) before delivery (r = 0.647, r = 0.547, p < 0.05); the same was true for plasma NPY ( r = 0.584, r = 0.705. p < 0.05). Conclusion: Leptin and NPY produced from fetus and placenta may regulate the fetal weight growth

  20. Copeptin, a surrogate marker for arginine vasopressin secretion, is associated with higher glucose and insulin concentrations but not higher blood pressure in obese men

    DEFF Research Database (Denmark)

    Asferg, C L; Andersen, Ulrik Bjørn; Linneberg, A;

    2014-01-01

    was associated with higher serum insulin concentrations (r = 0.26, P = 0.0085) and insulin resistance as assessed by the homeostasis assessment model (r = 0.28, P = 0.0051). CONCLUSIONS: Plasma copeptin, a surrogate marker for arginine vasopressin secretion, is higher in obese men compared with normal...... distribution. METHODS: In 103 obese men (mean age ± standard deviation: 49.4 ± 10.2 years) and 27 normal weight control men (mean age: 51.5 ± 8.4 years), taking no medication, we measured 24-h ambulatory blood pressure, fasting blood concentrations of copeptin, lipids, glucose and insulin, and determined body...... weight men, and is associated with abnormalities in glucose and insulin metabolism, but not with higher blood pressure or an android fat distribution in obese men....

  1. Profile of blood glucose and ultrastucture of beta cells pancreatic islet in alloxan compound induced rats

    OpenAIRE

    I Nyoman Suarsana; Priosoeryanto, B P; M. Bintang; T. Wresdiyati

    2010-01-01

    Diabetes is marked by elevated levels of blood glucose, and progressive changes of the structure of pancreatic islet histopathology. The objective of this research was to analyse the glucose level and histophatological feature in pancreatic islet in alloxan compound induced rats. A total of ten male Spraque Dawley rats of 2 months old were used in this study. The rats were divided into two groups: (1) negative control group (K-), and (2) positif induced alloxan group (diabetic group =DM). The...

  2. Blood Glucose Control in Type 1 Diabetes With a Bihormonal Bionic Endocrine Pancreas

    OpenAIRE

    Russell, Steven J.; El-Khatib, Firas H.; Nathan, David M.; Magyar, Kendra L.; Jiang, John; Damiano, Edward R.

    2012-01-01

    OBJECTIVE To test whether safe and effective glycemic control could be achieved in type 1 diabetes using a bihormonal bionic endocrine pancreas driven by a continuous glucose monitor in experiments lasting more than two days and including six high-carbohydrate meals and exercise as challenges to glycemic control. RESEARCH DESIGN AND METHODS Six subjects with type 1 diabetes and no endogenous insulin secretion participated in two 51-h experiments. Blood glucose was managed with a bionic endocr...

  3. The Association between Concentrations of Green Tea and Blood Glucose Levels

    OpenAIRE

    Maruyama, Koutatsu; ISO, Hiroyasu; Sasaki, Satoshi; Fukino, Yoko

    2008-01-01

    Our objective was to examine whether habitual green tea consumption is associated with blood glucose levels and other biomarkers of glucose metabolism. We conducted a cross-sectional study of 35 male volunteers, 23–63 years old and residing in Shizuoka Prefecture in Japan. Biochemical data were measured and we conducted a questionnaire survey on health, lifestyle, and nutrition, as well as frequency of consumption and concentrations (1%, 2%, and 3%) of green tea. Men who consumed a 3% concent...

  4. Cerebral blood flow, oxygen and glucose metabolism with PET in progressive supranuclear palsy

    Energy Technology Data Exchange (ETDEWEB)

    Otsuka, Makoto; Ichiya, Yuici; Kuwabara, Yasuo (Kyushu Univ., Fukuoka (Japan). Faculty of Medicine) (and others)

    1989-11-01

    Cerebral blood flow, cerebral oxygen metabolic rate and cerebral glucose metabolic rate were measured with positron emission tomography (PET) in four patients with progressive supranuclear palsy (PSP). Decreased blood flow and hypometabolism of oxygen and glucose were found in both subcortical and cortical regions, particularly in the striatum including the head of the caudate nucleus and the frontal cortex. The coupling between blood flow and metabolism was preserved even in the regions which showed decreased blood flow and hypometabolism. These findings indicated the hypofunction, as revealed by decreased blood flow and hypometablolism on PET, both in the striatum and the frontal cortex, and which may underlie the pathophysiological mechanism of motor and mental disturbance in PSP. (author).

  5. Cerebral blood flow, oxygen and glucose metabolism with PET in progressive supranuclear palsy

    International Nuclear Information System (INIS)

    Cerebral blood flow, cerebral oxygen metabolic rate and cerebral glucose metabolic rate were measured with positron emission tomography (PET) in four patients with progressive supranuclear palsy (PSP). Decreased blood flow and hypometabolism of oxygen and glucose were found in both subcortical and cortical regions, particularly in the striatum including the head of the caudate nucleus and the frontal cortex. The coupling between blood flow and metabolism was preserved even in the regions which showed decreased blood flow and hypometabolism. These findings indicated the hypofunction, as revealed by decreased blood flow and hypometablolism on PET, both in the striatum and the frontal cortex, and which may underlie the pathophysiological mechanism of motor and mental disturbance in PSP. (author)

  6. Effects of blood sample handling procedures on measurable inflammatory markers in plasma, serum and dried blood spot samples

    DEFF Research Database (Denmark)

    Skogstrand, K.; Thorsen, P.; Vogel, I.;

    2008-01-01

    whole blood samples at low temperatures and rapid isolation of plasma and serum. Effects of different handling procedures for all markers studied are given. DBSS proved to be a robust and convenient way to handle samples for immunoassay analysis of inflammatory markers in whole blood Udgivelsesdato......The interests in monitoring inflammation by immunoassay determination of blood inflammatory markers call for information on the stability of these markers in relation to the handling of blood samples. The increasing use of stored biobank samples for such ventures that may have been collected and...... stored for other purposes, justifies the study hereof. Blood samples were stored for 0, 4, 24, and 48 h at 4 degrees C, room temperature (RT), and at 35 degrees C, respectively, before they were separated into serum or plasma and frozen. Dried blood spot samples (DBSS) were stored for 0, 1, 2, 3, 7, and...

  7. The efficacy of karkadeh tea in controlling post-prandial blood glucose levels

    DEFF Research Database (Denmark)

    Harrison, Adrian Paul; Cooper, Ross Gordon; Suliman, Moustafa Ahmed;

    2009-01-01

    obesity. Approach: In order to investigate the efficacy of "karkadeh" Roselle-Hibiscus sabdariffa tea as a means of reducing post-prandial blood glucose levels in human subjects, 10g of dried karkadeh was brewed in 500ml of water, allowed to infuse for 60 min and imbibed along with a high glycemic index...... food. Results: Data show that in one individual, karkadeh tea taken in connection with a carbohydrate based breakfast meal resulted in a significant increase (17%; p<0.01) in blood glucose level cf. an ordinary breakfast tea 60 min after ingestion. Moreover, in a study involving eight individuals, a...... slower rise to maximum levels and a greater Area Under the Curve (AUC) in terms of blood glucose was noted for the karkadeh tea plus a high glycemic index food cf. that of water imbibition with an identical high glycemic food. Conclusions/Recommendations: Karkadeh tea appears to have an efffect in terms...

  8. Diabetes mellitus, fasting blood glucose concentration, and risk of vascular disease

    DEFF Research Database (Denmark)

    Sarwar, N; Gao, P; Seshasai, S R Kondapally;

    2010-01-01

    BACKGROUND: Uncertainties persist about the magnitude of associations of diabetes mellitus and fasting glucose concentration with risk of coronary heart disease and major stroke subtypes. We aimed to quantify these associations for a wide range of circumstances. METHODS: We undertook a meta...... markers. HRs for coronary heart disease were higher in women than in men, at 40-59 years than at 70 years and older, and with fatal than with non-fatal disease. At an adult population-wide prevalence of 10%, diabetes was estimated to account for 11% (10-12%) of vascular deaths. Fasting blood glucose......-analysis of individual records of diabetes, fasting blood glucose concentration, and other risk factors in people without initial vascular disease from studies in the Emerging Risk Factors Collaboration. We combined within-study regressions that were adjusted for age, sex, smoking, systolic blood pressure...

  9. Disposable glucose test strip for whole blood with integrated sensing/diffusion-limiting layer

    Energy Technology Data Exchange (ETDEWEB)

    Chen Zhencheng [Department of Biomedical Engineering, School of Info-Physics and Geomatics Engineering, Central South University, Changsha 410083 (China); Fang Cheng, E-mail: fangpingchuan@163.co [Department of Biomedical Engineering, School of Info-Physics and Geomatics Engineering, Central South University, Changsha 410083 (China); Wang Hongyan; He Jishan [Department of Biomedical Engineering, School of Info-Physics and Geomatics Engineering, Central South University, Changsha 410083 (China)

    2009-12-30

    A disposable glucose test strip with an integrated sensing/diffusion-limiting layer was developed. A formulation containing filler, glucose oxidase and electronic mediator was screen-printed over two carbon electrodes to form an integrated sensing/diffusion-limiting layer. On rehydration, the integrated layer does not break up, but swells to form a gelled and three-dimensional meshy reaction zone on the surface of the underlying conductive elements in which reactants and mediator move freely, but interfering species such as red blood cells containing oxygenated hemoglobin are excluded. On the same time, the integrated layer maintains a rate of permeation of the analyte through it with a variation of less than 10% at temperatures ranging from 15 deg. C to 42 deg. C. This biosensor is substantially insensitive to interferents and essentially independent to relevant temperature, which provides a more reliable reading of actual blood glucose value in human whole blood.

  10. Specificity of noninvasive blood glucose sensing using optical coherence tomography technique: a pilot study

    International Nuclear Information System (INIS)

    Noninvasive monitoring of blood glucose concentration in diabetic patients would significantly reduce complications and mortality associated with this disease. In this paper, we experimentally and theoretically studied specificity of noninvasive blood glucose monitoring with the optical coherence tomography (OCT) technique. OCT images and signals were obtained from skin of Yucatan micropigs and New Zealand rabbits. Obtained results demonstrate that: (1) several body osmolytes may change the refractive index mismatch between the interstitial fluid (ISF) and scattering centres in tissue, however the effect of the glucose is approximately one to two orders of magnitude higher; (2) an increase of the ISF glucose concentration in the physiological range (3-30 mM) may decrease the scattering coefficient by 0.22% mM-1 due to cell volume change; (3) stability of the OCT signal slope is dependent on tissue heterogeneity and motion artefacts; and (4) moderate skin temperature fluctuations (±1 deg. C) do not decrease accuracy and specificity of the OCT-based glucose sensor, however substantial skin heating or cooling (several deg. C) significantly change the OCT signal slope. These results suggest that the OCT technique may provide blood glucose concentration monitoring with sufficient specificity under normal physiological conditions

  11. Blood glucose responses of diabetes mellitus type II patients to some local fruits.

    Science.gov (United States)

    Guevarra, M T; Panlasigui, L N

    2000-12-01

    To determine the glucose responses of diabetes mellitus type II subjects to fruits, four locally available fruits (containing 25 g of available carbohydrates per serving portion) of chico, mango, pineapple, and papaya were tested among ten type II diabetic subjects, using wheat bread as the control. Results of the in vivo test indicated that chico and mango had significantly lower (P ≤ 0.05) blood glucose areas compared to wheat bread. Chico and mango also had a much lower glycemic index (GI), 57 and 59, respectively, compared to pineapple, 73 and papaya, 86. Differences in glucose responses to fruits and their varying GI are attributed to the amount of fiber, type and amount of sugars found, presence of antinutrients, acidity and physical characteristics of the fruits when chewed. The high fiber content of chico (7.9%), its fructose content (5.3%), its grainy texture when chewed and the presence of antinutrients (saponin, sapotin and achrasaponin) may contribute to its slow digestion and absorption. The low GI and blood glucose response of mango may be because of its fructose content (3.0%), acidity content (malic, citric and tartaric) and its phytic acid content (0.03%). Furthermore, starch, which is a possible factor contributing to low GI, is present in chico (0.8%) and mango (0.3%). Pineapple and papaya, the test fruits that elicited higher blood glucose responses and GI, have much lower fiber contents, less acids and contain glucose and sucrose sugars. PMID:24394507

  12. Investigating pipeline and state of the art blood glucose biosensors to formulate next steps.

    Science.gov (United States)

    Aggidis, Anthony G A; Newman, Jeffrey D; Aggidis, George A

    2015-12-15

    Ten years on from a review in the twentieth issue of this journal, this contribution assess the direction research in the field of glucose sensing for diabetes is headed and various technologies to be seen in the future. The emphasis of this review was placed on the home blood glucose testing market. After an introduction to diabetes and glucose sensing, this review analyses state of the art and pipeline devices; in particular their user friendliness and technological advancement. This review complements conventional reviews based on scholarly published papers in journals. PMID:26143465

  13. Impact of epidemic rates of diabetes on the Chinese blood glucose testing market.

    Science.gov (United States)

    Hu, Jamie; Zhang, Xian-En

    2011-09-01

    China has become the country with the largest diabetes mellitus population in the world since the 1990s. About 100 million diabetes cases have been diagnosed since 2008. Handheld blood glucose meters and test strips are urgently needed for daily patient measurement. The glucose monitor with a screen-printed carbon-based glucose electrode has been in commercial production since 1994. Since then, approximately 20 companies have been involved in manufacturing and marketing meters and test strips in China. The current market and production volume and updates on technology issues are discussed in this article. PMID:22027332

  14. Low Red Blood Cell Vitamin C Concentrations Induce Red Blood Cell Fragility: A Link to Diabetes Via Glucose, Glucose Transporters, and Dehydroascorbic Acid

    Directory of Open Access Journals (Sweden)

    Hongbin Tu

    2015-11-01

    Full Text Available Strategies to prevent diabetic microvascular angiopathy focus on the vascular endothelium. Because red blood cells (RBCs are less deformable in diabetes, we explored an original concept linking decreased RBC deformability to RBC ascorbate and hyperglycemia. We characterized ascorbate concentrations from human and mouse RBCs and plasma, and showed an inverse relationship between RBC ascorbate concentrations and deformability, measured by osmotic fragility. RBCs from ascorbate deficient mice were osmotically sensitive, appeared as spherocytes, and had decreased β-spectrin. These aberrancies reversed with ascorbate repletion in vivo. Under physiologic conditions, only ascorbate's oxidation product dehydroascorbic acid (DHA, a substrate for facilitated glucose transporters, was transported into mouse and human RBCs, with immediate intracellular reduction to ascorbate. In vitro, glucose inhibited entry of physiologic concentrations of dehydroascorbic acid into mouse and human RBCs. In vivo, plasma glucose concentrations in normal and diabetic mice and humans were inversely related to respective RBC ascorbate concentrations, as was osmotic fragility. Human RBC β-spectrin declined as diabetes worsened. Taken together, hyperglycemia in diabetes produced lower RBC ascorbate with increased RBC rigidity, a candidate to drive microvascular angiopathy. Because glucose transporter expression, DHA transport, and its inhibition by glucose differed for mouse versus human RBCs, human experimentation is indicated.

  15. A comprehensive evaluation of strip performance in multiple blood glucose monitoring systems.

    Science.gov (United States)

    Katz, Laurence B; Macleod, Kirsty; Grady, Mike; Cameron, Hilary; Pfützner, Andreas; Setford, Steven

    2015-05-01

    Accurate self-monitoring of blood glucose is a key component of effective self-management of glycemic control. Accurate self-monitoring of blood glucose results are required for optimal insulin dosing and detection of hypoglycemia. However, blood glucose monitoring systems may be susceptible to error from test strip, user, environmental and pharmacological factors. This report evaluated 5 blood glucose monitoring systems that each use Verio glucose test strips for precision, effect of hematocrit and interferences in laboratory testing, and lay user and system accuracy in clinical testing according to the guidelines in ISO15197:2013(E). Performance of OneTouch(®) VerioVue™ met or exceeded standards described in ISO15197:2013 for precision, hematocrit performance and interference testing in a laboratory setting. Performance of OneTouch(®) Verio IQ™, OneTouch(®) Verio Pro™, OneTouch(®) Verio™, OneTouch(®) VerioVue™ and Omni Pod each met or exceeded accuracy standards for user performance and system accuracy in a clinical setting set forth in ISO15197:2013(E). PMID:25702769

  16. System accuracy of blood glucose monitoring systems: impact of use by patients and ambient conditions.

    Science.gov (United States)

    Schmid, Christina; Haug, Cornelia; Heinemann, Lutz; Freckmann, Guido

    2013-10-01

    For self-monitoring of blood glucose by people with diabetes, the reliability of the measured blood glucose values is a prerequisite in order to ensure correct therapeutic decisions. Requirements for system accuracy are defined by the International Organization for Standardization (ISO) in the standard EN ISO 15197:2003. However, even a system with high analytical quality is not a guarantee for accurate and reliable measurement results. Under routine life conditions, blood glucose measurement results are affected by several factors. First, the act of performing measurements as well as the handling of the system may entail numerous possible error sources, such as traces of glucose-containing products on the fingertips, the use of deteriorated test strips, or the incorrect storage of test strips. Second, ambient and sampling conditions such as high altitude, partial pressure of oxygen, ambient temperature, and the use of alternate test sites can have an influence on measurement results. Therefore, the user-friendliness of a system and the quality of the manufacturer's labeling to reduce the risk of handling errors are also important aspects in ensuring reliable and accurate measurement results. In addition, the analytical performance of systems should be less prone to user errors and ambient conditions. Finally, people with diabetes must be aware of the information and instructions in the manufacturer's labeling and must be able to measure and interpret blood glucose results correctly. PMID:23883407

  17. Lanthanides' enhancing absorption of insulin and reduction of blood glucose of rat by pulmonary administration

    Institute of Scientific and Technical Information of China (English)

    2000-01-01

    To explore the possibility of lanthanides as the enhancer of insulin absorption, the promoting effects of lanthanide ions on the absorption of intrapulmonary delivered insulin and reduction of blood glucose were investigated by means of two approaches, preadministration and coadministration of lanthanide ions with insulin. The results indicate that, compared with the results of those given insulin only, lanthanide ions can effectively enhance the level of insulin and the reduction of glucose level in blood. These effects are dependent on lanthanide species and their concentrations. The mechanism was discussed.

  18. Effect of dietary fiber in lowering serum glucose and body weight in sprague dawley rats

    Directory of Open Access Journals (Sweden)

    Muhammad Imran Chohan

    2011-08-01

    Full Text Available Introduction:The present study evaluated the hypoglycemic perspectives and weight loss significance of dietary fiber. Dietary fiber was supplemented in commercial wheat flour (atta for the preparation of chapaties, a staple diet of South Asia. Male Sprague Dawley rats (n = 100 were randomly divided into 4 diet groups (n = 25 per group. The control group was fed basal diet that included commercial wheat flour chapati, cornstarch, corn oil, salt and vitamin mixture in such a way that 10% of the protein was available from the final diet. To the basal diet of other 3 groups, chapaties supplemented with 2% guar gum (GG 2%, 3% guar gum (GG 3% and 5% chickpea + 1% guar gum (CP5%+GG1% were added, respectively. All diets were fed to the rats for a period of 8 weeks to perceive the impact of respective compositions. Rats fed on CP 5% + GG1%, showed maximum glucose reduction (14.57% followed by GG 3% (11.64% and GG 2% (9.60% as compared to control diet. Likewise, rats fed on 3% GG showed maximum decline (7.90% in body weight. It was concluded that chapaties prepared from selected treatments provide an additional dietary fiber that could be supportive for diabetic and obese individuals.Results:The results indicated that addition of dietary fiber influenced the physical characteristics of chapati non-significantly. Maximum glucose concentration was found to be 112.50 mg/dL in control group followed by 101.70 and 99.41 mg/dL in groups fed on guar gum 2% and guar gum 3%, respectively. Lowest glucose concentration (96.11 mg/dL was observed in rats fed on the combination of chickpea 5 %+ guar gum 1%. Maximum serum protein concentration was found to be 6.39 g/dL in rats fed on combination of chickpea 5 % + guar gum 1% whilst the remaining three groups showed non significant variations with respect to each other. Means for serum Functional Foods in Health and Disease 2011; 8:261-278 protein were 6.33, 6.30 and 6.32 g/dL for control, guar gum 3%, and guar gum 2

  19. Clinical utility and accuracy of a blood glucose meter for the detection of neonatal hypoglycemia

    International Nuclear Information System (INIS)

    Objective: To determine the accuracy and reliability of a glucometer in comparison to hexokinase method in detecting neonatal hypoglycemia. Subjects and Methods: All neonates presenting with known risk factors or suggestive clinical features were screened for hypoglycemia by using capillary blood on Accutrend alpha glucometer. Simultaneously the venous blood glucose values were done on Hitachi 902 autoanalyser by hexokinase method. A level of 40 mg/dl or less was taken as neonatal hypoglycemia. Results: A total of 292-paired samples were taken from 223 neonates. Hypoglycemia was detected in 112 samples (38.4%). Correlation of glucometer values with laboratory values of blood glucose levels was excellent throughout the range with coefficient of correlation (r) of 0.976 (p-value < 0.001). For blood glucose values equal or less than 40 mg/dl, r was 0.547 (p-value < 0.001). The instrument used showed a sensitivity of 98% and specificity of 93% to detect neonatal hypoglycemia (equal or less than 40 mg/dl) with a positive predictive value of 88% and negative predictive value 99%. Conclusion: The blood glucose reflectance meter can be a useful and accurate instrument for screening and detecting neonatal hypoglycemia in symptomatic babies under stress. All low values by glucometer should be promptly analyzed and confirmed by chemical laboratory. (author)

  20. Low perfusion index affects the difference in glucose level between capillary and venous blood

    Directory of Open Access Journals (Sweden)

    Acar N

    2014-11-01

    Full Text Available Nurdan Acar,1 Hamit Ozcelik,1 Arif Alper Cevik,1 Engin Ozakin,1 Goknur Yorulmaz,2 Nur Kebapci,2 Ugur Bilge,3 Muzaffer Bilgin4 1Emergency Department, 2Endocrinology Department, 3Family Medicine Department, 4Biostatistics Department, Medical School, Eskisehir Osmangazi University, Eskisehir, Turkey Aim: In emergency cases, finger stick testing is primarily used to check the blood glucose value of patients since it takes longer to obtain the venous value. In critical patients, under conditions that cause an increase in metabolic state and level of stress, there occurs considerable difference in glucose levels between capillary and venous measurements. This study aimed to investigate the comparability of capillary and venous glucose values, according to the perfusion index level obtained with the Masimo Radical-7® device, in critical patients aged 18 years and over.Method: We conducted this prospective and observational study in the emergency department of the Eskisehir Osmangazi University hospital between November 3, 2008 and February 2, 2009.Results: The blood glucose of 300 critical patients was checked by finger stick in the emergency unit. The participants with normal vital signs had perfusion index between 0 and 5; the results obtained by the two methods were more consistent for perfusion index values of 6 and over. The results were most consistent in aged participants with normal vital sign findings and low perfusion index and in young patients with high perfusion index. In the cases where at least one of the vital signs was abnormal, the glucose values obtained by the two methods were more consistent when the perfusion index was 6 or over. In this group, independently from the perfusion index value, the consistency was higher in younger patients compared with aged patients.Conclusion: In the emergency department, perfusion index value measured by Masimo Radical-7 and capillary blood glucose levels can serve in blood sugar management in

  1. Blood glucose levels and cortical thinning in cognitively normal, middle-aged adults.

    Science.gov (United States)

    Wennberg, Alexandra M V; Spira, Adam P; Pettigrew, Corinne; Soldan, Anja; Zipunnikov, Vadim; Rebok, George W; Roses, Allen D; Lutz, Michael W; Miller, Michael M; Thambisetty, Madhav; Albert, Marilyn S

    2016-06-15

    Type II diabetes mellitus (DM) increases risk for cognitive decline and is associated with brain atrophy in older demented and non-demented individuals. We investigated (1) the cross-sectional association between fasting blood glucose level and cortical thickness in a sample of largely middle-aged, cognitively normal adults, and (2) whether these associations were modified by genes associated with both lipid processing and dementia. To explore possible modifications by genetic status, we investigated the interaction between blood glucose levels and the apolipoprotein E (APOE) ε4 allele and the translocase of the outer mitochondrial membrane (TOMM) 40 '523 genotype on cortical thickness. Cortical thickness measures were based on mean thickness in a subset of a priori-selected brain regions hypothesized to be vulnerable to atrophy in Alzheimer's disease (AD) (i.e., 'AD vulnerable regions'). Participants included 233 cognitively normal subjects in the BIOCARD study who had a measure of fasting blood glucose and cortical thickness measures, quantified by magnetic resonance imaging (MRI) scans. After adjustment for age, sex, race, education, depression, and medical conditions, higher blood glucose was associated with thinner parahippocampal gyri (B=-0.002; 95% CI -0.004, -0.0004) and temporal pole (B=-0.002; 95% CI -0.004, -0.0001), as well as reduced average thickness over AD vulnerable regions (B=-0.001; 95% CI -0.002, -0.0001). There was no evidence for greater cortical thinning in ε4 carriers of the APOE gene or in APOE ε3/3 individuals carrying the TOMM40 VL/VL genotypes. When individuals with glucose levels in the diabetic range (≥126mg/dL), were excluded from the analysis, the associations between glucose levels and cortical thickness were no longer significant. These findings suggest that glucose levels in the diabetic range are associated with reduced cortical thickness in AD vulnerable regions as early as middle age. PMID:27206882

  2. Sesame and flaxseed oil: nutritional quality and effects on serum lipids and glucose in rats

    Directory of Open Access Journals (Sweden)

    Rita de Cássia Avellaneda Guimarães

    2013-03-01

    Full Text Available This study evaluated the nutritional value of sesame and flaxseed oils and their effects on the lipid and glucose profile of rats fed diets containing different fat combinations. Fatty acid composition, refractive index, and iodine and saponification values were analyzed to characterize the oils. In the biological assay, Wistar rats were fed different diets, whose fat composition consisted of varying combinations of flaxseed oil, sesame oil, and animal fat. The primary constituents of the sesame oil were oleic (28.6%, linoleic (28.4%, and lauric acid (14.6%; for the flaxseed oil they were alpha-linolenic (39.90%, oleic (17.97% and linoleic acid (12.25%. The iodine and saponification values of the oils were within the reference range. Rats fed flaxseed oil-based diets had the lowest serum cholesterol values, whereas rats fed diets with flaxseed oil + sesame oil + animal fat had the highest glucose levels. HDL levels decreased significantly with flaxseed oil. Sesame and flaxseed oils are sources of polyunsaturated fatty acids (PUFA, and the flaxseed oil-based diet had a hypocholesterolemic effect, whereas sesame oil showed oxidative stability since it contains high levels of monounsaturated and saturated fatty acids.

  3. Functionalized graphene oxide for clinical glucose biosensing in urine and serum samples.

    Science.gov (United States)

    Veerapandian, Murugan; Seo, Yeong-Tai; Shin, Hyunkyung; Yun, Kyusik; Lee, Min-Ho

    2012-01-01

    A novel clinical glucose biosensor fabricated using functionalized metalloid-polymer (silver-silica coated with polyethylene glycol) hybrid nanoparticles on the surface of a graphene oxide nanosheet is reported. The cyclic voltammetric response of glucose oxidase modification on the surface of a functionalized graphene oxide electrode showed a surface-confined reaction and an effective redox potential near zero volts, with a wide linearity of 0.1-20 mM and a sensitivity of 7.66 μA mM(-1) cm(-2). The functionalized graphene oxide electrode showed a better electrocatalytic response toward oxidation of H(2)O(2) and reduction of oxygen. The practical applicability of the functionalized graphene oxide electrode was demonstrated by measuring the peak current against multiple urine and serum samples from diabetic patients. This new hybrid nanoarchitecture combining a three-dimensional metalloid-polymer hybrid and two-dimensional graphene oxide provided a thin solid laminate on the electrode surface. The easy fabrication process and retention of bioactive immobilized enzymes on the functionalized graphene oxide electrode could potentially be extended to detection of other biomolecules, and have broad applications in electrochemical biosensing. PMID:23269871

  4. THE EFFECT OF GINGER ROOT POWDER (zingiber officinale SUPPLEMENTATION ON BROILER CHICKS PERFORMANCE, BLOOD AND SERUM CONSTITUENTS

    Directory of Open Access Journals (Sweden)

    W.B. ZOMRAWI

    2012-11-01

    Full Text Available A study using one hundred and twenty eight unsexed day old broiler chicks (Ross 308 32 birds/treatment with four replicates was conducted to evaluate the effect of ginger root powder as natural feed additives on growth performance, blood and serum constituents of broiler chickens. Four dietary treatments were formulated to meet the nutrient requirements of broiler chick containing ginger root powder at levels 0%, 0.5%, 1% and 1.5%. Result showed that significant decreased (P0.05 in feed conversion ratio among all dietary treatments. Treatments had significant decreased (P0.05 were observed in dressing percentage. There were no significant effect (P>0.05 on serum glucose, total protein and creatinine. Significant differences were observed in serum triglyceride and cholesterol levels. There were no significant differences (P>0.05 among all dietary treatments in Hb percentage, PCV percentage, TRBcs, MCV, MCH and MCHC percentage. The results showed that the inclusion of ginger root powder at levels 0.5% and1% in the diet , had lowering effect on cholesterol levels, and the chick may tolerate up to 1.5% without adverse effect on growth performance and blood parameters.

  5. The effects of coffee on serum lipids and blood pressure in a UK population.

    OpenAIRE

    Lancaster, T; Muir, J; Silagy, C

    1994-01-01

    Data on coffee consumption were collected for 1074 adults attending for health checks in the OXCHECK study, to assess associations with serum lipids and blood pressure. Of the sample, 70.5% drank coffee, largely instant. Coffee had no significant effects on total or high-density lipoprotein (HDL) cholesterol or blood pressure, and was negatively correlated with serum triglycerides. The type of coffee drunk in the UK does not adversely effect these cardiovascular risk factors.

  6. Effect of Butea monosperma Lam. leaves and bark extracts on blood glucose in streptozotocin-induced severely diabetic rats

    Directory of Open Access Journals (Sweden)

    Faiyaz Ahmed

    2012-01-01

    Full Text Available Background: Diabetes mellitus is a chronic metabolic disorder that has significant impact on the health, quality of life and life expectancy, as well as on the health care system. Butea monosperma Lam. Kuntze (Fabaceae, commonly known as palash, is widely used in the treatment of various diseases and disorders including diabetes. Materials and Methods: The present study was planned to evaluate the antidiabetic effect of aqueous extracts of B. monosperma leaves and bark in streptozotocin-induced severely diabetic rats. The animals were divided into four groups, with each consisting of six rats, viz. control, diabetic control, leaf extract-treated and bark extract-treated groups. Treatment was continued for 6 weeks. The biochemical estimations included blood glucose and serum insulin levels. Histopathology of pancreas was also performed. Results: The results indicated that both leaf and bark extracts of B. monosperma produced insignificant antihyperglycemic activity. The leaf and bark extracts reduced blood glucose to an extent of 28% and 11%, respectively. It was also evidenced that both leaf and bark extracts did not increase insulin synthesis or secretion and did not improve pancreatic architecture as reflected by the histopathologic studies. Conclusions: The findings of the study emphasize that B. monosperma does not possess significant antidiabetic activity in severe experimental diabetes at the dosage tested.

  7. A new biosensor for glucose determination in serum based on up-converting fluorescence resonance energy transfer.

    Science.gov (United States)

    Peng, Jianhong; Wang, Yuhui; Wang, Jialan; Zhou, Xin; Liu, Zhihong

    2011-10-15

    In this work, a new glucose sensor based on up-converting fluorescence resonance energy transfer (UC-FRET) was developed. Up-converting phosphors (UCPs, NaYF(4): Yb, Er), which were covalently labeled with Concanavalin A (ConA), were used as the energy donor with thiolated β-cyclodextrins (SH-β-CDs) functionalized gold nanoparticles as the energy acceptor. Due to the combination between ConA and SH-β-CDs, the energy donor and the acceptor were brought to close proximity, resulting in the quenching of the fluorescence of UCPs by gold nanoparticles. In the presence of glucose which competed with SH-β-CDs towards the binding sites of ConA, the biosensor (UCPs-ConA-SH-β-CDs-Au) was decomposed and the energy donor was separated from the acceptor. Therefore, the fluorescence of UCPs was restored dependent on the concentration of glucose. The increase of UCPs fluorescence intensity was proportional to glucose concentration within the range from 0.4 μM to 10μM in aqueous buffer, with a limit of detection (LOD) of 0.043 μM. A same linear range of glucose concentration was obtained in a human serum matrix (which was pretreated and thus contained no glucose) with a slightly higher LOD (0.065 μM). The glucose sensor was applied to real human serum samples with the results consistent with that of a classic hexokinase (HK) method, indicating that the UC-FRET biosensor was competent for directly sensing glucose in serum samples without optical interference, which benefited from the near infrared (NIR) excitation nature of UCPs. The results of this work suggested that the UC-FRET technique could be a promising alternative for detecting biomolecules in complex biological sample matrixes for diagnostic purposes. PMID:21852101

  8. Effects of MDMA on blood glucose levels and brain glucose metabolism

    OpenAIRE

    Soto-Montenegro, M L; Vaquero, Juan José; Arango, C.; Ricaurte, G.; García-Barreno, P; Desco, Manuel

    2007-01-01

    Purpose This study was designed to assess changes in glucose metabolism in rats administered single or repeated doses of MDMA. Methods Two different experiments were performed: (1) A single-dose study with four groups receiving 20 mg/kg, 40 mg/kg, saline or heat, and (2) a repeated-dose study with two groups receiving three doses, at intervals of 2 h, of 5 mg/kg or saline. Rats were imaged using a dedicated small-animal PET scanner 1 h after single-dose administration or 7 days after repeated...

  9. Optimal Control of Blood Glucose: The Diabetic Patient or the Machine?

    OpenAIRE

    Brown, Larry; Edelman, Elazer R.

    2010-01-01

    In this issue of Science Translational Medicine, El-Khatib et al. describe a “closed-loop” bi-hormonal artificial pancreas, designed to avert episodes of low blood sugar in patients with insulin-dependent diabetes. We discuss the benefits and challenges of therapy directed at tight control of blood glucose and ask whether this and similar technological breakthroughs can address as yet unanswered questions in the biology of diabetes.

  10. Changes in blood pressure and body weight over ten years in men selected for glucose intolerance.

    OpenAIRE

    Jarrett, R J; Keen, H; Murrells, T

    1987-01-01

    Relative changes in body weight and blood pressure over ten years of observation are reported in men recruited for a trial of therapy in relation to the natural history of glucose intolerance. Half were recommended a diet restricting carbohydrate to 120 g daily (diet group) and half were recommended to 'limit use of table sugar' (no diet). In both groups average weight and blood pressure fell over the 12 to 18 months after treatment allocation, the decline being proportionately greater for bo...

  11. Influence of Serum and Glucose Additives on Survival of Actinobacillus pleuropneumoniae Aerosolized from the Freeze-Dried State

    OpenAIRE

    Hensel, Andreas

    1994-01-01

    Serum and/or glucose added to Actinobacillus pleuropneumoniae suspensions before freeze-drying significantly increased survival rates of bacteria in aerosols. Aerosols with predictable numbers of viable bacteria can be made as required in an aerosol infection model. Sucrose supplementation of impinger fluids increased recovery of viable A. pleuropneumoniae.

  12. Melatonin Signaling Controls the Daily Rhythm in Blood Glucose Levels Independent of Peripheral Clocks.

    Directory of Open Access Journals (Sweden)

    Sharon Owino

    Full Text Available Melatonin is rhythmically secreted by both the pineal gland and retina in a circadian fashion, with its peak synthesis occurring during the night. Once synthesized, melatonin exerts its effects by binding to two specific G-protein coupled receptors-melatonin receptor type 1(MT1 and melatonin receptor type 2(MT2. Recent studies suggest the involvement of MT1 and MT2 in the regulation of glucose homeostasis; however the ability of melatonin signaling to impart timing cues on glucose metabolism remains poorly understood. Here we report that the removal of MT1 or MT2 in mice abolishes the daily rhythm in blood glucose levels. Interestingly, removal of melatonin receptors produced small effects on the rhythmic expression patterns of clock genes within skeletal muscle, liver, and adipose tissue. Taken together, our data suggest that the loss of the daily rhythm in blood glucose observed in MT1(-/- and MT2(-/- mice does not occur as a consequence of 'disrupted' clocks within insulin sensitive tissues. Finally our results highlight a diurnal contribution of melatonin receptor signaling in the daily regulation of blood glucose levels.

  13. Early blood glucose profile and neurodevelopmental outcome at two years in neonatal hypoxic-ischaemic encephalopathy

    LENUS (Irish Health Repository)

    Nadeem, Montasser

    2011-02-04

    Abstract Background To examine the blood glucose profile and the relationship between blood glucose levels and neurodevelopmental outcome in term infants with hypoxic-ischaemic encephalopathy. Methods Blood glucose values within 72 hours of birth were collected from 52 term infants with hypoxic-ischaemic encephalopathy. Hypoglycaemia [< 46.8 mg\\/dL (2.6 mmol\\/L)] and hyperglycaemia [> 150 mg\\/dL (8.3 mmol\\/L)] were correlated to neurodevelopmental outcome at 24 months of age. Results Four fifths of the 468 blood samples were in the normoglycaemic range (392\\/468:83.8%). Of the remaining 76 samples, 51.3% were in the hypoglycaemic range and (48.7%) were hyperglycaemic. A quarter of the hypoglycaemic samples (28.2%:11\\/39) and a third of the hyperglycaemic samples (32.4%:12\\/37) were recorded within the first 30 minutes of life. Mean (SD) blood glucose values did not differ between infants with normal and abnormal outcomes [4.89(2.28) mmol\\/L and 5.02(2.35) mmol\\/L, p value = 0.15] respectively. In term infants with hypoxic-ischaemic encephalopathy, early hypoglycaemia (between 0-6 hours of life) was associated with adverse outcome at 24 months of age [OR = 5.8, CI = 1.04-32)]. On multivariate analysis to adjust for grade of HIE this association was not statistically significant. Late hypoglycaemia (6-72 hours of life) was not associated with abnormal outcome [OR = 0.22, CI (0.04-1.14)]. The occurrence of hyperglycaemia was not associated with adverse outcome. Conclusion During the first 72 hours of life, blood glucose profile in infants with hypoxic-ischaemic encephalopathy varies widely despite a management protocol. Early hypoglycaemia (0-6 hours of life) was associated with severe HIE, and thereby; adverse outcome.

  14. Early blood glucose profile and neurodevelopmental outcome at two years in neonatal hypoxic-ischaemic encephalopathy.

    LENUS (Irish Health Repository)

    Nadeem, Montasser

    2012-01-31

    BACKGROUND: To examine the blood glucose profile and the relationship between blood glucose levels and neurodevelopmental outcome in term infants with hypoxic-ischaemic encephalopathy. METHODS: Blood glucose values within 72 hours of birth were collected from 52 term infants with hypoxic-ischaemic encephalopathy. Hypoglycaemia [< 46.8 mg\\/dL (2.6 mmol\\/L)] and hyperglycaemia [> 150 mg\\/dL (8.3 mmol\\/L)] were correlated to neurodevelopmental outcome at 24 months of age. RESULTS: Four fifths of the 468 blood samples were in the normoglycaemic range (392\\/468:83.8%). Of the remaining 76 samples, 51.3% were in the hypoglycaemic range and (48.7%) were hyperglycaemic. A quarter of the hypoglycaemic samples (28.2%:11\\/39) and a third of the hyperglycaemic samples (32.4%:12\\/37) were recorded within the first 30 minutes of life. Mean (SD) blood glucose values did not differ between infants with normal and abnormal outcomes [4.89(2.28) mmol\\/L and 5.02(2.35) mmol\\/L, p value = 0.15] respectively. In term infants with hypoxic-ischaemic encephalopathy, early hypoglycaemia (between 0-6 hours of life) was associated with adverse outcome at 24 months of age [OR = 5.8, CI = 1.04-32)]. On multivariate analysis to adjust for grade of HIE this association was not statistically significant. Late hypoglycaemia (6-72 hours of life) was not associated with abnormal outcome [OR = 0.22, CI (0.04-1.14)]. The occurrence of hyperglycaemia was not associated with adverse outcome. CONCLUSION: During the first 72 hours of life, blood glucose profile in infants with hypoxic-ischaemic encephalopathy varies widely despite a management protocol. Early hypoglycaemia (0-6 hours of life) was associated with severe HIE, and thereby; adverse outcome.

  15. Higher blood glucose level associated with body mass index and gut microbiota in elderly people

    Science.gov (United States)

    Sepp, Epp; Kolk, Helgi; Lõivukene, Krista; Mikelsaar, Marika

    2014-01-01

    Background Some dominant bacterial divisions of the intestines have been linked to metabolic diseases such as overweight and diabetes. Objective A pilot study aimed to evaluate the relations between the culturable intestinal bacteria with body mass index (BMI) and some principal cellular and metabolic markers of blood in people older than 65. Design Altogether 38 generally healthy elderly people were recruited: ambulatory (n=19) and orthopedic surgery (n=19). Questionnaires on general health, anthropometric measurements, routine clinical and laboratory data, and quantitative composition of cultivable gut microbiota were performed. Results Blood glucose level was positively correlated with BMI (r=0.402; p=0.014). Higher blood glucose level had negative correlation with relative share of intestinal anaerobic bacteria such as bacteroides (r=−0.434; p=0.0076) and gram-positive anaerobic cocci (r=−0.364; p=0.027). In contrast, the relative share of bifidobacteria (r=0.383; p=0.019) and staphylococci (r=0.433; p=0.008) was positively correlated to blood glucose level. In elderly people, a higher blood glucose concentration was predicted by the reduction of the anaerobes’ proportion (adj. sex, age, and BMI R2=0.192, p=0.028) and that of Bacteroides sp. (adj. R2=0.309, p=0.016). Conclusion A tight interplay between increased BMI, level of blood glucose, and the reduced proportion of cultivable bacteroides is taking place in the gut microbiota of elderly people. PMID:24936169

  16. Higher blood glucose level associated with body mass index and gut microbiota in elderly people

    Directory of Open Access Journals (Sweden)

    Epp Sepp

    2014-06-01

    Full Text Available Background: Some dominant bacterial divisions of the intestines have been linked to metabolic diseases such as overweight and diabetes. Objective: A pilot study aimed to evaluate the relations between the culturable intestinal bacteria with body mass index (BMI and some principal cellular and metabolic markers of blood in people older than 65. Design: Altogether 38 generally healthy elderly people were recruited: ambulatory (n=19 and orthopedic surgery (n=19. Questionnaires on general health, anthropometric measurements, routine clinical and laboratory data, and quantitative composition of cultivable gut microbiota were performed. Results: Blood glucose level was positively correlated with BMI (r=0.402; p=0.014. Higher blood glucose level had negative correlation with relative share of intestinal anaerobic bacteria such as bacteroides (r=−0.434; p=0.0076 and gram-positive anaerobic cocci (r=−0.364; p=0.027. In contrast, the relative share of bifidobacteria (r=0.383; p=0.019 and staphylococci (r=0.433; p=0.008 was positively correlated to blood glucose level. In elderly people, a higher blood glucose concentration was predicted by the reduction of the anaerobes’ proportion (adj. sex, age, and BMI R2=0.192, p=0.028 and that of Bacteroides sp. (adj. R2=0.309, p=0.016. Conclusion: A tight interplay between increased BMI, level of blood glucose, and the reduced proportion of cultivable bacteroides is taking place in the gut microbiota of elderly people.

  17. Non-invasive blood glucose monitoring with Raman spectroscopy: prospects for device miniaturization

    Science.gov (United States)

    Wróbel, M. S.

    2016-01-01

    The number of patients with diabetes has reached over 350 million, and still continues to increase. The need for regular blood glucose monitoring sparks the interest in the development of modern detection technologies. One of those methods, which allows for noninvasive measurements, is Raman spectroscopy. The ability of infrared light to penetrate deep into tissues allows for obtaining measurements through the skin without its perforation. This paper presents the limitations and possibilities of non-invasive blood glucose monitoring with Raman spectroscopy. Especially focusing on the possibilities for device miniaturization. Such device incorporates a Raman spectrometer, a fiber-optical probe, and a computing device (microcontroller, smartphone, etc.) which calculates the glucose concentration using specialized algorithms. Simplification of device design, as well as turbidity correction technique and a new proposed method of synchronized detection are described.

  18. Correlation of salivary glucose, blood glucose and oral candidal carriage in the saliva of type 2 diabetics: A case-control study

    Directory of Open Access Journals (Sweden)

    Satish Kumar

    2014-01-01

    Full Text Available Objectives: To study the correlation between blood glucose levels and salivary glucose levels in type 2 diabetic patients, to study the relationship between salivary glucose levels and oral candidal carriage in type 2 diabetic patients and to determine whether salivary glucose levels could be used as a noninvasive tool for the measurement of glycemic control in type 2 diabetics. Study Design: The study population consisted of three groups: Group 1 consisted of 30 controlled diabetics and Group 2 consisted of 30 uncontrolled diabetics based on their random nonfasting plasma glucose levels. Group 3 consisted of 30 healthy controls. Two milliliters of peripheral blood was collected for the estimation of random nonfasting plasma glucose levels and glycosylated hemoglobin (HbA1c. Unstimulated saliva was collected for the estimation of salivary glucose. Saliva was collected by the oral rinse technique for the estimation of candidal counts. Results: The salivary glucose levels were significantly higher in controlled and uncontrolled diabetics when compared with controls. The salivary candidal carriage was also significantly higher in uncontrolled diabetics when compared with controlled diabetics and nondiabetic controls. The salivary glucose levels showed a significant correlation with blood glucose levels, suggesting that salivary glucose levels can be used as a monitoring tool for predicting glycemic control in diabetic patients. Conclusion: The present study found that estimation of salivary glucose levels can be used as a noninvasive, painless technique for the measurement of diabetic status of a patient in a dental set up. Increased salivary glucose levels leads to increased oral candidal carriage; therefore, oral diagnosticians are advised to screen the diabetic patients for any oral fungal infections and further management.

  19. Bihormonal control of blood glucose in people with type 1 diabetes

    DEFF Research Database (Denmark)

    Batora, Vladimir; Tárnik, Marían; Murgaš, Ján;

    2015-01-01

    This paper presents a bihormonal artificial pancreas (AP) for people with type 1 diabetes (T1D) designed to provide a safe blood glucose control with minimal use of glucagon. The control algorithm uses insulin as well as glucagon to prevent hyper- and hypoglycemia. We employ a novel prediction...... predicts hypoglycemia. Predictions utilize an ARMAX model describing glucose-insulin and glucose-glucagon dynamics. The model parameters are estimated from basic patient-specific data. A continuous glucose monitor provides feedback. We test the control algorithm using a simulation model with time......-based activation of glucagon administration. The control algorithm consists of a Kalman filter, an insulin infusion model predictive controller (MPC), a proportional-derivative (PD) controller for glucagon infusion, and a meal time insulin bolus calculator. The PD controller is activated if the Kalman filter...

  20. The Effect of Blood Glucose Self-Monitoring Among Inmates With Diabetes.

    Science.gov (United States)

    Hunter Buskey, Robin N; Mathieson, Kathleen; Leafman, Joan S; Feinglos, Mark N

    2015-10-01

    The increasing prevalence and risk of complications from diabetes necessitate patient participation and attentiveness to select appropriate foods, perform regular physical activity, and be active in diabetes management and self-maintenance. Diabetes is often largely asymptomatic; consequently, early diagnosis and treatment are necessary. Inmates are a unique population challenged by the increased prevalence of chronic conditions including diabetes. Diabetes standards for inmates contain diagnostic and treatment management guidelines that incorporate personal glucose monitoring for insulin users. In December 2009, the Federal Bureau of Prisons initiated a program to distribute glucose meters to insulin-dependent inmates to facilitate self-monitoring blood glucose. The purpose of this study was to evaluate the effect of these glucose meters on hemoglobin A1c levels. PMID:26276137

  1. Feasibility of non-invasive optical blood-glucose detection using overtone circular dichroism

    CERN Document Server

    Hokr, Brett H; Meng, Zhaokai; Petrov, Georgi I; Yakovlev, Vladislav V

    2016-01-01

    Diabetes is one of the most debilitating and costly diseases currently plaguing humanity. It is a leading cause of death and dismemberment in the world, and we know how to treat it. Accurate, continuous monitoring and control of blood glucose levels via insulin treatments are widely known to mitigate the majority of detrimental effects caused by the disease. The primary limitation of continuous glucose monitoring is patient non-compliance due to the unpleasant nature of "finger-stick" testing methods. This limitation can be largely, or even completely, removed by non-invasive testing methods. In this report, we demonstrate the vibrational overtone circular dichroism properties of glucose and analyze its use as a method of non-invasive glucose monitoring, capable of assuaging this trillion dollar scourge.

  2. A Method for Manipulating Blood Glucose and Measuring Resulting Changes in Cognitive Accessibility of Target Stimuli.

    Science.gov (United States)

    Prokosch, Marjorie L; Hill, Sarah E

    2016-01-01

    Much research in social psychology has investigated the impact of bodily energy need on cognition and decision-making. As such, blood glucose, the body's primary energy source, has been of special interest to researchers for years. Fluctuations in blood glucose have been linked to a variety of changes in cognitive and behavioral processes, such as self-control, political attitudes, and eating behavior. To help meet growing interest in the links between bodily energy need and these processes, this manuscript offers a simple methodology to experimentally manipulate blood glucose using a fasting procedure followed by administration of a sugar-sweetened, unsweetened, or artificially-sweetened beverage. This is followed by presentation of a method for measuring resulting changes in implicit cognition using a lexical decision-task. In this task, participants are asked to identify whether strings of letters are words or non-words and response latencies are recorded. Sample results from a recent publication are presented as an example of the applications for the experimental manipulation of blood glucose and the lexical decision task measures. PMID:27585282

  3. Cinnamon intake lowers fasting blood glucose: an updated meta-analysis

    Science.gov (United States)

    OBJECTIVE – To determine if meta-analysis of recent clinical studies of cinnamon intake by people with Type II diabetes and/or prediabetes resulted in significant changes in fasting blood glucose. RESEARCH DESIGN AND METHODS -- Published clinical studies were identified using a literature search (P...

  4. What do professionals recommend regarding the frequency of self-monitoring of blood glucose?

    NARCIS (Netherlands)

    Hortensius, J.; Kleefstra, N.; Houweling, S. T.; van der Bijl, J. J.; Gans, R. O. B.; Bilo, H. J. G.

    2012-01-01

    Background: Patients' adherence to guidelines regarding self-monitoring of blood glucose (SMBG) is limited. However, there are no previous reports about the recommendations that are given in clinical practice concerning SMBG. The aim of this study was to investigate what healthcare providers recomme

  5. Blood Glucose Monitoring as a Teaching Tool for Endocrinology: A New Perspective

    Science.gov (United States)

    Moats, Robert K., II

    2009-01-01

    The education of new allied health professionals and nurses in proper endocrine evaluation and care has become critical in recent years, especially considering the greatly increased prevalence of diabetes in adults and children. The evaluation of blood glucose levels in human volunteers over time is a powerful teaching tool for endocrinology that…

  6. Higher Blood Glucose within the Normal Range Is Associated with More Severe Strokes

    DEFF Research Database (Denmark)

    Martin, Rolf J; Ratan, Rajiv R; Reding, Michael J;

    2012-01-01

    Background. Higher fasting blood glucose (FBG) concentrations in the hyperglycemic range are associated with more severe strokes. Whether this association also extends into patients with FBG in the normoglycemic range is unclear. We studied the association of stroke severity and FBG in normoglyce...

  7. Comprehensive Experiment--Clinical Biochemistry: Determination of Blood Glucose and Triglycerides in Normal and Diabetic Rats

    Science.gov (United States)

    Jiao, Li; Xiujuan, Shi; Juan, Wang; Song, Jia; Lei, Xu; Guotong, Xu; Lixia, Lu

    2015-01-01

    For second year medical students, we redesigned an original laboratory experiment and developed a combined research-teaching clinical biochemistry experiment. Using an established diabetic rat model to detect blood glucose and triglycerides, the students participate in the entire experimental process, which is not normally experienced during a…

  8. A novel fiber composite ingredient incorporated into a beverage and bar blunts postprandial serum glucose and insulin responses: a randomized controlled trial.

    Science.gov (United States)

    O'Connor, Lauren E; Campbell, Wayne W

    2016-03-01

    Previous research supports that consumption of resistant starch and guar gum independently influences insulin-mediated glucose responses to meals. This research assessed a novel co-processed fiber composite (FC) ingredient comprising whole-grain high-amylose maize flour and viscous guar gum on glucose and insulin responses to co-consumed and subsequent meals in humans. It was hypothesized that a smoothie-type beverage or a cold-pressed snack bar containing the FC would blunt and sustain serum glucose and insulin postprandial responses compared with maltodextrin (MD). The beverage and bar were assessed in 2 separate studies using identical protocols. Young, nondiabetic, nonobese adults participated in 2 testing days (randomized crossover design) separated by at least 1 week for both food forms. On each testing day, the FC or MD product was consumed with a low-fiber standardized breakfast followed by a low-fiber standardized lunch (with no FC or MD) 4 hours later. Blood samples were collected at baseline and incrementally throughout the 8-hour testing day. One-tailed paired t tests were performed to compare treatment areas under the curve, and a doubly repeated-measures analysis of variance was performed to compare treatment responses at individual time points (P< .05, Bonferroni corrected). The FC blunted the postprandial glucose and insulin responses compared with MD, including a robust glucose and insulin response reduction after breakfast and a continued modest glycemic second-meal reduction after lunch in both the beverage and the bar. These findings support the use of this novel whole-grain FC ingredient in a beverage or bar for insulin-mediated glucose control in young healthy adults. PMID:26923512

  9. Impairment of brain endothelial glucose transporter by methamphetamine causes blood-brain barrier dysfunction

    Directory of Open Access Journals (Sweden)

    Murrin L Charles

    2011-03-01

    Full Text Available Abstract Background Methamphetamine (METH, an addictive psycho-stimulant drug with euphoric effect is known to cause neurotoxicity due to oxidative stress, dopamine accumulation and glial cell activation. Here we hypothesized that METH-induced interference of glucose uptake and transport at the endothelium can disrupt the energy requirement of the blood-brain barrier (BBB function and integrity. We undertake this study because there is no report of METH effects on glucose uptake and transport across the blood-brain barrier (BBB to date. Results In this study, we demonstrate that METH-induced disruption of glucose uptake by endothelium lead to BBB dysfunction. Our data indicate that a low concentration of METH (20 μM increased the expression of glucose transporter protein-1 (GLUT1 in primary human brain endothelial cell (hBEC, main component of BBB without affecting the glucose uptake. A high concentration of 200 μM of METH decreased both the glucose uptake and GLUT1 protein levels in hBEC culture. Transcription process appeared to regulate the changes in METH-induced GLUT1 expression. METH-induced decrease in GLUT1 protein level was associated with reduction in BBB tight junction protein occludin and zonula occludens-1. Functional assessment of the trans-endothelial electrical resistance of the cell monolayers and permeability of dye tracers in animal model validated the pharmacokinetics and molecular findings that inhibition of glucose uptake by GLUT1 inhibitor cytochalasin B (CB aggravated the METH-induced disruption of the BBB integrity. Application of acetyl-L-carnitine suppressed the effects of METH on glucose uptake and BBB function. Conclusion Our findings suggest that impairment of GLUT1 at the brain endothelium by METH may contribute to energy-associated disruption of tight junction assembly and loss of BBB integrity.

  10. Tracking blood glucose and predicting prediabetes in Chinese children and adolescents: a prospective twin study.

    Directory of Open Access Journals (Sweden)

    Guoying Wang

    Full Text Available We examined the tracking of blood glucose, the development of prediabetes, and estimated their genetic contributions in a prospective, healthy, rural Chinese twin cohort. This report includes 1,766 subjects (998 males, 768 females aged 6-21 years at baseline who completed a 6-year follow-up study. Oral glucose tolerance test was performed for all subjects at both baseline and follow-up. We found that subjects with low fasting plasma glucose (FPG or 2 h post-load glucose (PG levels at baseline tended to remain at the low level at follow-up. Subjects in the top tertile of baseline plasma glucose tended to have a higher risk of developing prediabetes at follow-up compared to the low tertile: in males, 37.6% vs. 27.6% for FPG and 37.2% vs. 25.7% for 2hPG, respectively; in females, 31.0% vs. 15.4% for FPG and 28.9% vs. 15.1% for 2 h PG, respectively. Genetic factors explained 43% and 41% of the variance of FPG, and 72% and 47% for impaired fasting glucose for males and females, respectively; environmental factors substantially contribute to 2hPG status and impaired glucose tolerance. In conclusion, in this cohort of healthy rural Chinese children and adolescents, we demonstrated that both FPG and 2hPG tracked well and was a strong predictor of prediabetes. The high proportion of children with top tertile of blood glucose progressed to prediabetes, and the incidence of prediabetes has a male predominance. Genetic factors play more important role in fasting than postload status, most of which was explained by unique environmental factors.

  11. Estimation of free copper ion concentrations in blood serum using T1 relaxation rates

    Science.gov (United States)

    Blicharska, Barbara; Witek, Magdalena; Fornal, Maria; MacKay, Alex L.

    2008-09-01

    The water proton relaxation rate constant R1 = 1/ T1 (at 60 MHz) of blood serum is substantially increased by the presence of free Cu 2+ ions at concentrations above normal physiological levels. Addition of chelating agents to serum containing paramagnetic Cu 2+ nulls this effect. This was demonstrated by looking at the effect of adding a chelating agent—D-penicillamine (D-PEN) to CuSO 4 and CuCl 2 aqueous solutions as well as to rabbit blood serum. We propose that the measurement of water proton spin-lattice relaxation rate constants before and after chelation may be used as an alternative approach for monitoring the presence of free copper ions in blood serum. This method may be used in the diagnosis of some diseases (leukaemia, liver diseases and particularly Wilson's disease) because, in contrast to conventional methods like spectrophotometry which records the total number of both bound and free ions, the proton relaxation technique is sensitive solely to free paramagnetic ions dissolved in blood serum. The change in R1 upon chelation was found to be less than 0.06 s -1 for serum from healthy subjects but greater than 0.06 s -1 for serum from untreated Wilson's patients.

  12. Gum Guar fiber associated with fructose reduces serum triacylglycerol but did not improve the glucose tolerance in rats

    Directory of Open Access Journals (Sweden)

    Motoyama Caio SM

    2010-10-01

    Full Text Available Abstract Introduction The increased intake of dietary fructose can be associated with alterations on energy homeostasis and lipid/carbohydrate metabolism, such as insulin resistance and dislipidemia. On the other hand, the ingestion of soluble fiber gum guar could improve benefic mechanism on glucose tolerance and lipids profile. Objective The aim of the present study were to investigate the effects of the supplemental feeding partially hydrolyzed gum guar on glucose and lipid homeostasis, in rats fed with fructose solution. Methods The study was performed on thirty day-old male Wistar rats randomly assigned into four groups: control(C or treated with fructose (F-20%, fiber (FB-5%, or fructose plus fiber (F-20% + FB-5% = FF solution for 30 days on glucose tolerance (OGTT, triacylglycerol concentration in the liver by chloroform/methanol method, glucose, triacylglycerol and total cholesterol serum concentration by assayed by enzymatic colorimetric method, insulin receptor (IR concentration in the liver by Western Blotting. Results The total body weight gain was not different between groups; in regards of total caloric intake, in the F group was significantly higher and in the FB group was lower than other groups. The triacylglycerol concentration in the liver of FF group was significantly higher than F group, the triacylglycerol concentration in the serum was higher the F group compared with other groups. The OGTT reveal impaired on glucose tolerance in the F, FB, FF compared with C. The IR concentration in the liver was lower in the F, FB, FF compared with C, no significant difference was observed between groups for IR concentration in the gastrocnemius muscle. No significant difference was observed between groups for carcass fat content and serum total cholesterol. Conclusion Fructose induced important alterations on glucose tolerance and lipid metabolism, despite of fiber showed reversion of part this alterations. The association fructose plus

  13. ELISA with double antigen sandwich for screening specific serum anti-TP antibody in blood donors

    International Nuclear Information System (INIS)

    Objective: To select a sensitive and specific laboratory examination suitable for screening serum anti-TP antibody in blood donors. Methods: The serum anti-TP antibody in 11271 blood donors were detected using ELISA with double antigen sandwich and the outcomes were compared with those using RPR assay. The conflicting specimen were confirmed by repeating the test with TPHA assay. Results: The positive rates of serum anti-TP antibody by ELISA with double antigen sandwich and RPR was 0.36% (41/11271) and 0.26% (29/11271), respectively. The coincidence of the detecting outcomes by ELISA with double antigen sandwich and RPR with TPHA was 97.5% (40/41) and 63.41%(26/41) respectively. Conclusion: Compared with RPR assay, ELISA with double antigen sandwich has higher sensibility and specificity for screening serum anti-TP antibody in blood donors

  14. The effect of food with different glycaemic index on the blood glucose level

    Directory of Open Access Journals (Sweden)

    Lenka Kouřimská

    2015-08-01

    Full Text Available Blood glucose levels are affected by many factors including the type of foods consumed, processing technology and cooking method. Hormone insulin lowers blood glucose to its constant level, while glucagon, growth hormone, adrenalin and glucocorticoids have the opposite effect. High steepness of the blood glucose level rise after meals may be unfavourable for the organism. Sugars are transferred into the blood at different speeds according to the type of food. Therefore the aim of this study was to confirm experimentally the effect of food on blood glucose levels in men and women of different ages. Two types of low, medium and high-glycaemic index (GI foods were given to 4 men and 4 women of different age (from 35 to 65 years. All volunteers were healthy, slightly overweight, and without any regular sporting activity. None of them had any idea about their daily carbohydrates consumption and what the term glycaemic index meant. The volunteers came to the GI determination fasted in the morning. Their rise in blood glucose level was monitored by glucometer before the meal and after 1 and 2 hours of the consumption of baked potatoes (GI 85, white bread bun (GI 70, boiled potatoes (GI 64, rye bread (GI 62, potato dumplings (GI 52 and white cooked spaghetti (GI 41. Fasting blood sugar levels of volunteers highly depended on their age (p <0.0001 and gender (p <0.0001. The blood glucose values increased with age and were higher in men than in women. Significant influence of food GI on blood glucose levels in both men and women in all the age categories was observed (p <0.0001. An interaction between age and gender was also statistically highly significant (p <0.0001. One hour after consuming food the blood glucose values were significantly different from the values of fasting (p = 0.0035. The differences of these values did not depend on the age (p = 0.0574 and sex (p = 0.8256 of volunteers, but there was a significant difference on the GI value of food

  15. PREANALITIC AND INTERPRETATION BLOOD GLUCOSE FOR DIAGNOSE DIABETIC MELITUS

    Directory of Open Access Journals (Sweden)

    Ida Bagus Wayan Kardika

    2013-10-01

    Full Text Available Normal 0 false false false EN-US X-NONE X-NONE Diabetes mellitus (DM is a metabolic disorder disease which has been characterised by chronic hyperglycemia as well as abnormalities in the metabolism of carbohydrates, fats and proteins caused by abnormalities of insulin secretion, insulin resistance or both. The Diagnoses of diabetes mellitus in addition based on the clinical aspects of which include anamnesis, physical examination and diagnoses is required in the form of investigations the laboratory examination. The simplest laboratory examination is the examination of blood sugar. The stages of preanalitic and the interpretation of results examination of the blood sugar is worth noting in order to obtain meaningful results so that a diagnosis of diabetes mellitus can be enforced and for monitoring treatment outcomes. /* Style Definitions */ table.MsoNormalTable {mso-style-name:"Table Normal"; mso-tstyle-rowband-size:0; mso-tstyle-colband-size:0; mso-style-noshow:yes; mso-style-priority:99; mso-style-qformat:yes; mso-style-parent:""; mso-padding-alt:0in 5.4pt 0in 5.4pt; mso-para-margin:0in; mso-para-margin-bottom:.0001pt; mso-pagination:widow-orphan; font-size:11.0pt; font-family:"Calibri","sans-serif"; mso-ascii-font-family:Calibri; mso-ascii-theme-font:minor-latin; mso-fareast-font-family:"Times New Roman"; mso-fareast-theme-font:minor-fareast; mso-hansi-font-family:Calibri; mso-hansi-theme-font:minor-latin; mso-bidi-font-family:"Times New Roman"; mso-bidi-theme-font:minor-bidi;}

  16. Comparison of serum leptin, glucose, total cholesterol and total protein levels in fertile and repeat breeder cows

    Directory of Open Access Journals (Sweden)

    Saime Guzel

    2014-12-01

    Full Text Available In the present study we measured serum glucose, leptin, total cholesterol and total protein concentrations in repeat breeder cows and compared them with fertile cows. For this aim, 20 repeat breeder cows and 20 fertile cows were used as material. Repeat breeder cows were found to have lower levels of leptin and glucose as compared with fertile ones. No significant differences in total cholesterol and total protein levels were observed between the two groups. No significant correlation of leptin with glucose, total cholesterol and total protein was observed in fertile and repeat breeder cows. Low concentrations of glucose and leptin can have some effects on reproductive problems as repeat breeder and help to understand potential mechanisms impairing fertility in repeat breeder cows.

  17. Optical diagnosis of dengue virus infection in human blood serum using Raman spectroscopy

    International Nuclear Information System (INIS)

    We present the optical diagnosis of dengue virus infection in human blood serum using Raman spectroscopy. Raman spectra were acquired from 18 blood serum samples using a laser at 532 nm as the excitation source. A multivariate regression model based on partial least-squares regression is developed that uses Raman spectra to predict dengue infection with leave-one-sample-out cross validation. The prediction of dengue infection by our model yields correlation coefficient r2 values of 0.9998 between the predicted and reference clinical results. The model was tested for six unknown human blood sera and found to be 100% accurate in accordance with the clinical results. (letter)

  18. Effect of a serum factor on IgE-mediated histamine release from whole blood.

    OpenAIRE

    Tanizaki,Yoshiro; Komagoe,Haruki; Sudo,Michiyasu; Morinaga, Hiroshi; Nakagawa,Saburo; Kitani,Hikaru; Takahashi, Kiyoshi; Kimura,Ikuro

    1984-01-01

    IgE-mediated histamine release from whole blood was analyzed in 44 patients with bronchial asthma by observing maximum present release and dose-response curves of histamine release induced by anti-IgE and house dust extract. The maximum histamine release from whole blood induced by anti-IgE correlated with total serum IgE levels. There was a close correlation between allergen-induced release from whole blood and the serum levels of specific IgE antibodies. In the maximum histamine release fro...

  19. Blood concentrations of lactate, glucose and corticosterone in dispersing hatchling sea turtles

    Directory of Open Access Journals (Sweden)

    Carla M. Pereira

    2012-11-01

    Natal dispersal of sea turtles is an energetically demanding activity that is fuelled primarily by aerobic metabolism. However, during intense exercise reptiles can use anaerobic metabolism to supplement their energy requirements. We assessed anaerobic metabolism in dispersing hatchling loggerhead and flatback turtles by measuring the concentrations of blood lactate during crawling and at different times during the first four hours of their frenzy swim. We also measured concentrations of blood glucose and corticosterone. Blood lactate (12.13 to 2.03 mmol/L, glucose (6.25 to 3.8 mmol/L and corticosterone (8.13 to 2.01 ng/mL concentrations decreased significantly over time in both loggerhead and flatback hatchlings and no significant differences were found between the species. These results indicate that anaerobic metabolism makes a significant contribution to the dispersal phase of hatchling sea turtles during the beach crawl and the first few hours of the frenzy swim.

  20. A signal processing application for evaluating self-monitoring blood glucose strategies in a software agent model.

    Science.gov (United States)

    Wang, Zhanle; Paranjape, Raman

    2015-07-01

    We propose the signal processing technique of calculating a cross-correlation function and an average deviation between the continuous blood glucose and the interpolation of limited blood glucose samples to evaluate blood glucose monitoring frequency in a self-aware patient software agent model. The diabetic patient software agent model [1] is a 24-h circadian, self-aware, stochastic model of a diabetic patient's blood glucose levels in a software agent environment. The purpose of this work is to apply a signal processing technique to assist patients and physicians in understanding the extent of a patient's illness using a limited number of blood glucose samples. A second purpose of this work is to determine an appropriate blood glucose monitoring frequency in order to have a minimum number of samples taken that still provide a good understanding of the patient's blood glucose levels. For society in general, the monitoring cost of diabetes is an extremely important issue, and these costs can vary tremendously depending on monitoring approaches and monitoring frequencies. Due to the cost and discomfort associated with blood glucose monitoring, today, patients expect monitoring frequencies specific to their health profile. The proposed method quantitatively assesses various monitoring protocols (from 6 times per day to 1 time per week) in nine predefined categories of patient agents in terms of risk factors of health status and age. Simulation results show that sampling 6 times per day is excessive, and not necessary for understanding the dynamics of the continuous signal in the experiments. In addition, patient agents in certain conditions only need to sample their blood glucose 1 time per week to have a good understanding of the characteristics of their blood glucose. Finally, an evaluation scenario is developed to visualize this concept, in which appropriate monitoring frequencies are shown based on the particular conditions of patient agents. This base line can

  1. Detecting free hemoglobin in blood plasma and serum with luminescent terbium complexes

    OpenAIRE

    Morgner, F.; Lecointre, A.; Charbonniere, L.J.; Lohmannsroben, H.G.

    2015-01-01

    Hemolysis, the rupturing of red blood cells, can result from numerous medical conditions (in vivo) or occur after collecting blood specimen or extracting plasma and serum out of whole blood (in vitro). In clinical laboratory practice, hemolysis can be a serious problem due to its potential to bias detection of various analytes or biomarkers. Here we present the first "mix-and-measure'' method to assess the degree of hemolysis in biosamples using luminescence spectroscopy. Luminescent terbium ...

  2. Glucose tolerance, blood lipid, insulin and glucagon concentration after single or continuous administration of aspartame in diabetics.

    Science.gov (United States)

    Okuno, G; Kawakami, F; Tako, H; Kashihara, T; Shibamoto, S; Yamazaki, T; Yamamoto, K; Saeki, M

    1986-04-01

    A nutritive sweetener, aspartame (L-aspartyl-L-phenylalanine methylester) was administered orally to normal controls and diabetic patients in order to evaluate effects on blood glucose, lipids and pancreatic hormone secretion. An oral glucose tolerance test was also performed in the same subjects as a control study of aspartame administration. In 7 normal controls and 22 untreated diabetics, a single dose of 500 mg aspartame, equivalent to 100 g glucose in sweetness, induced no increase in blood glucose concentration. Rather, a small but significant decrease in blood glucose was noticed 2 or 3 h after administration. The decrease in blood glucose was found to be smallest in the control and became greater as the diabetes increased in severity. No significant change in blood insulin or glucagon concentration during a 3-h period was observed in either the controls or the diabetics. The second study was designed to determine the effects of 2 weeks' continuous administration of 125 mg aspartame, equal in sweetness to the mean daily consumption of sugar (20-30 g) in Japan, to 9 hospitalized diabetics with steady-state glycemic control. The glucose tolerance showed no significant change after 2 weeks' administration. Fasting, 1 h and 2 h postprandial blood glucose, blood cholesterol, triglyceride and HDL-cholesterol were also unaffected. From these and other published results, aspartame would seem to be a useful alternative nutrient sweetener for patients with diabetes mellitus. PMID:3522147

  3. Monitoring of glucose, salt and pure water in human whole blood: An in vitro study.

    Science.gov (United States)

    Imran, Muhammad; Ullah, Hafeez; Akhtar, Munir; Sial, Muhammad Aslam; Ahmed, Ejaz; Durr-E-Sabeeh; Ahmad, Mukhtar; Hussain, Fayyaz

    2016-07-01

    Designing and implementation of non-invasive methods for glucose monitoring in blood is main focus of biomedical scientists to provide a relief from skin puncturing of diabete patient. The objective of this research work is to investigate the shape deformations and the aggregation of red blood cells (RBCs) in the human blood after addition of three different analytes i) (0mM-400mM: Range) of glucose (C(6)H(12)O(6)), ii) (0mM-400mM: range) of pure salt (NaCl) and iii) (0mM- 350mM: range) of pure water (H(2)O). We have observed that the changes in the shape of individual cells from biconcave discs to spherical shapes and eventually the lysis of the cells at optimum concentration of glucose, salts and pure water. This demonstration also provides a base line to facilitate diabetes during partial diagnosis and monitoring of the glucose levels qualitatively both in research laboratories and clinical environment. PMID:27393437

  4. Auto-Immune Hemolytic Anemia in Patient who his Serum React with all ABO Blood Group

    Directory of Open Access Journals (Sweden)

    A Pourazar

    2004-12-01

    Full Text Available There are several irregular antibodies associated with various blood group systems which may cause some problems during blood cross matching in transfusion. The atypical antibodies are included auto and alloantibodies such as anti-I, anti-HI, anti-P… . In order to detect these antibodies, generally the agglutination reaction technique and anti-human-globulin (coombs tests would be performed and a panel of identified red blood cells will use if necessary for further investigation. During our work, we encountered with one serum sample that showed agglutination reaction with all the blood groups (A, B, O, and AB. We tested pooled red blood cells with OI group of adult and pooled cord red blood cells of Oi group with the patient serum. it was shown that the serum was reactive with OI but not with Oi. For confirmation of the result, the sample was sent to Institute of Immunohematology (I.I.H., India. The report approved that the serum contained anti-I specificity. To solve the transfusion problem for this patient, the recommendation is using the blood group with minimum coombs titration if the patient life is in threatened. Further investigations disclosed that the patient had leukemia. Keywords: Anti-I, Ii antigen, Allo-Autoantibody.

  5. Blood glucose lowering effect of ophiopogonis tuber extract and mechanism of anti-insulin-resistance

    Directory of Open Access Journals (Sweden)

    Meng NING

    2013-01-01

    Full Text Available Objective  To study the hypoglycemic effect and insulin sensitization mechanism of ophiopogonis tuber extracts on the 3T3-L1-induced adipocytes, and also in rats with reproduction of type 2 diabetes mellitus (T2DM. Methods  3T3-L1 cells were induced and differentiated into adipocytes. After the intervention with ophiopogonpolysaccharide (OPSR and ophiopogonin (OPG, glucose consuming rate was detected for screening the extracts which may have effective hypoglycemic effects. The insulin resistance (IR adipocyte model was established by dexamethasone induction, and then it was treated with OPSR. The protein expression levels of leptin, adiponectin and resistin were detected by Western blotting. The T2DM rat model was reproduced and then treated with OPSR for 4 weeks. Body weight (BW, triglyeride (TG, fasting blood glucose (FBG and fasting insulin (FINs of the rats were measured respectively. Results  OPSR in dosage of 0.5-50mg/L promoted glucose consumption of adipocytes in a dose-dependent manner, the glucose consumption ratios were 32.27%, 75.14% and 90.47% respectively. OPG of 50mg/L showed very weak activity with glucose consumption ratio of only 8.49%. OPSR could significantly promote the protein expression of leptin and adiponectin, and showed an inhibitory effect on the protein expression of resistin (P<0.05. After treatment with OPSR for 4 weeks, the BW of rats increased obviously, while TG, FBG and HOMA-IR decreased significantly (P<0.05 or P<0.01. Conclusions  OPSR may promote glucose transport and utilization of adipocytes, decrease the level of FBG and TG, and improve the condition of IR in T2DM rats. The mechanism of blood glucose lowering effect may be attributed to secretion of adipokines, such as leptin, adiponectin and resistin by IR adipocytes.

  6. Determination of Blood Glucose Concentration by Using Wavelet Transform and Neural Networks

    Directory of Open Access Journals (Sweden)

    Vajravelu Ashok

    2013-03-01

    Full Text Available Background: Early and non-invasive determination of blood glucose level is of great importance. We aimed to present a new technique to accurately infer the blood glucose concentration in peripheral blood flow using non-invasive optical monitoring system.Methods: The data for the research were obtained from 900 individuals. Of them, 750 people had diabetes mellitus (DM. The system was designed using a helium neon laser source of 632.8 nm wavelength with 5mW power, photo detectors and digital storage oscilloscope. The laser beam was directed through a single optical fiber to the index finger and the scattered beams were collected by the photo detectors placed circumferentially to the transmitting fiber. The received signals were filtered using band pass filter and finally sent to a digital storage oscilloscope. These signals were then decomposed into approximation and detail coefficients using modified Haar Wavelet Transform. Back propagation neural and radial basis functions were employed for the prediction of blood glucose concentration.Results: The data of 450 patients were randomly used for training, 225 for testing and the rest for validation. The data showed that outputs from radial basis function were nearer to the clinical value. Significant variations could be seen from signals obtained from patients with DM and those without DM.Conclusion: The proposed non-invasive optical glucose monitoring system is able to predict the glucose concentration by proving that there is a definite variation in hematological distribution between patients with DM and those without DM.

  7. Effect of guar crispbread with cereal products and leguminous seeds on blood glucose concentrations of diabetics.

    OpenAIRE

    Jenkins, D J; Wolever, T M; Taylor, R H; Barker, H M; Fielden, H; Jenkins, A. L.

    1980-01-01

    To compare the effect on blood glucose concentrations of guar incorporated into crispbreads with that of unprocessed high-fibre foods groups of four to six diabetics took a total of seven test breakfasts on separate days. By comparison with a breakfast of wholemeal bread and cheese, guar crispbread combined with bread reduced the area under the glucose response curve to 51% (p < 0.05); bread and soya beans reduced the area to 65% (p < 0.05); guar crispbread with soya beans to 25% (p < 0.002);...

  8. "KETONURIA AND SERUM GLUCOSE OF FASTING PREGNANT WOMEN AT THE END OF A DAY IN RAMADAN"

    Directory of Open Access Journals (Sweden)

    M. Arab

    2004-08-01

    Full Text Available Moslem pregnant women are inclined to fast during the month of Ramadan. Ketonuria and hypoglycemia are harmful in pregnancy. The aim of this study was to find out whether clinical symptoms and/ or calorie deficiency of fasting pregnant women before Eftar (ending of the daily fast can accurately predict ketonuria and hypoglycemia. In a descriptive study, 185 volunteer fasting pregnant women in Ramadan of 1999 were tested just before Eftar taking into account their clinical symptoms, intake of the previous 24 hours and testing their ketonuria and serum glucose. The positive predictive values of clinical symptoms before breaking the fast for ketonuria and hypoglycemia were 32% and 56%, respectively. The negative predictive values of clinical symptoms for ketonuria and hypoglycemia were 70% and 33%. The positive predictive values of over 500 Kcal deficiency in ketonuria and hypoglycemia were 33% and 57%. Severe calorie deficiency (more than 2000 Kcal positively predicts ketonuria in 70% of cases and sufficient calorie intake (less than 500 Kcal deficiency negatively predicts ketonuria in 93% of women. Clinical symptoms and calorie intake were not appropriate criteria for predicting ketonuria and hypoglycemia except in asymptomatic or severely calorie deficient cases or those with sufficient calorie intake whose ketonuria was predicted accurately.

  9. Performance Evaluation and Labeling Comprehension of a New Blood Glucose Monitoring System with Integrated Information Management

    Science.gov (United States)

    List, Susan M; Starks, Nykole; Baum, John; Greene, Carmine; Pardo, Scott; Parkes, Joan L; Schachner, Holly C; Cuddihy, Robert

    2011-01-01

    Background This study evaluated performance and product labeling of CONTOUR® USB, a new blood glucose monitoring system (BGMS) with integrated diabetes management software and a universal serial bus (USB) port, in the hands of untrained lay users and health care professionals (HCPs). Method Subjects and HCPs tested subject's finger stick capillary blood in parallel using CONTOUR USB meters; deep finger stick blood was tested on a Yellow Springs Instruments (YSI) glucose analyzer for reference. Duplicate results by both subjects and HCPs were obtained to assess system precision. System accuracy was assessed according to International Organization for Standardization (ISO) 15197:2003 guidelines [within ±15 mg/dl of mean YSI results (samples system features and ease-of-use were evaluated by subject questionnaires. Results All subjects who completed the study (N = 74) successfully performed blood glucose measurements, connected the meter to a laptop computer, and used key features of the system. The system was accurate; 98.6% (146/148) of subject results and 96.6% (143/148) of HCP results exceeded ISO 15197:2003 criteria. All subject and HCP results were clinically accurate (97.3%; zone A) or associated with benign errors (2.7%; zone B). The majority of subjects rated features of the BGMS as “very good” or “excellent.” Conclusions CONTOUR USB exceeded ISO 15197:2003 system performance criteria in the hands of untrained lay users. Subjects understood the product labeling, found the system easy to use, and successfully performed blood glucose testing. PMID:22027308

  10. Effect of parenterally administered cystamine and gammaphos (WR-2721) on some biochemical parameters in dog blood serum

    International Nuclear Information System (INIS)

    The effects were studied of intravenous and intramuscular administration of radioprotectives cystamine and gammaphos in dogs on the biochemical parameters of the blood serum. The activities were studied of enzymes aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase, creatinine kinase, gamma-glutamyl transpeptidase, lactate dehydrogenase, malate dehydrogenase, sorbitol dehydrogenase and alpha-amylase. The contents were determined of total protein, albumin, bilirubin, urea nitrogen, creatinine, glucose, triglycerides, cholesterol, lipids, calcium, sodium and potassium. Cystamine was shown to be hepatotoxic. The intramuscular administration of gammaphos was found to be more advantageous than of cystamine. Only slight increase was observed in the activities of lactate dehydrogenase, malate dehydrogenase, and alpha-amylase. With cystamine, the changes in all biochemical parameters were most marked. (M.D.). 17 figs., 18 refs

  11. Molecular Weight Dependent Glucose Lowering Effect of Low Molecular Weight Chitosan Oligosaccharide (GO2KA1 on Postprandial Blood Glucose Level in SD Rats Model

    Directory of Open Access Journals (Sweden)

    Emmanouil Apostolidis

    2013-07-01

    Full Text Available This research investigated the effect of enzymatically digested low molecular weight (MW chitosan oligosaccharide on type 2 diabetes prevention. Three different chitosan oligosaccharide samples with varying MW were evaluated in vitro for inhibition of rat small intestinal α-glucosidase and porcine pancreatic α-amylase (GO2KA1; 10,000 Da. The in vitro results showed that all tested samples had similar rat α-glucosidase inhibitory and porcine α-amylase inhibitory activity. Based on these observations, we decided to further investigate the effect of all three samples at a dose of 0.1 g/kg, on reducing postprandial blood glucose levels in Sprague-Dawley (SD rat model after sucrose loading test. In the animal trial, all tested samples had postprandial blood glucose reduction effect, when compared to control, however GO2KA1 supplementation had the strongest effect. The glucose peak (Cmax for GO2KA1 and control was 152 mg/dL and 193 mg/dL, respectively. The area under the blood glucose-time curve (AUC for GO2KA1 and control was 262 h mg/dL and 305 h mg/dL, respectively. Furthermore, the time of peak plasma concentration of blood glucose (Tmax for GO2KA1 was significantly delayed (0.9 h compared to control (0.5 h. These results suggest that GO2KA1 could have a beneficial effect for blood glucose management relevant to diabetes prevention in normal and pre-diabetic individuals. The suggested mechanism of action is via inhibition of the carbohydrate hydrolysis enzyme α-glucosidase and since GO2KA1 (MW < 1000 Da had higher in vivo effect, we hypothesize that it is more readily absorbed and might exert further biological effect once it is absorbed in the blood stream, relevant to blood glucose management.

  12. Overnight Control of Blood Glucose in People with Type 1 Diabetes

    DEFF Research Database (Denmark)

    Boiroux, Dimitri; Duun-Henriksen, Anne Katrine; Schmidt, Signe;

    2012-01-01

    In this paper, we develop and test a Model Predictive Controller (MPC) for overnight stabilization of blood glucose in people with type 1 diabetes. The controller uses glucose measurements from a continuous glucose monitor (CGM) and its decisions are implemented by a continuous subcutaneous insulin...... infusion (CSII) pump. Based on a priori patient information, we propose a systematic method for computation of the model parameters in the MPC. Safety layers improve the controller robustness and reduce the risk of hypoglycemia. The controller is evaluated in silico on a cohort of 100 randomly generated...... patients with a representative intersubject variability. This cohort is simulated overnight with realistic variations in the insulin sensitivities and needs. Finally, we provide results for the first tests of this controller in a real clinic....

  13. Effects of long duration exercise on cognitive function, blood glucose, and counterregulatory hormones in male cyclists.

    Science.gov (United States)

    Grego, Fabien; Vallier, Jean-Marc; Collardeau, Maya; Bermon, Stéphane; Ferrari, Patricia; Candito, Mirande; Bayer, Pascale; Magnié, Marie-Noëlle; Brisswalter, Jeanick

    2004-07-01

    Electrocortical effects of long duration exercise on cognitive function were investigated by analyzing P300 amplitude and latency changes during a 3-h cycling exercise. P300 components were measured in 12 well-trained cyclists and blood glucose, cortisol, insulin, glycerol, and free fatty acids (FFAs) epinephrine and norepinephrine were analyzed. Results indicated that P300 components were affected by exercise with a temporary increase in amplitude between the 1st and the 2nd hour and an increase in latency after 2 h of exercise concomitant with some hormonal changes, including an increase in cortisol and epinephrine and a decrease in blood glucose. These findings suggest a combined effect of arousal and central fatigue on electrocortical indices of cognitive function during acute physical exercise. PMID:15196681

  14. Frequency and motives of blood glucose self-monitoring in type 1 diabetes

    DEFF Research Database (Denmark)

    Hansen, M V; Pedersen-Bjergaard, U; Heller, S R;

    2009-01-01

    AIMS: Recommendations for self-monitoring of blood glucose (SMBG) from the DCCT have not been implemented with the same rigour as recommendations for intensifying insulin therapy. We assessed the frequency of and motives for SMBG and compared SMBG behaviour with clinical, behavioural and demograp......AIMS: Recommendations for self-monitoring of blood glucose (SMBG) from the DCCT have not been implemented with the same rigour as recommendations for intensifying insulin therapy. We assessed the frequency of and motives for SMBG and compared SMBG behaviour with clinical, behavioural and...... hypoglycaemia and awareness of hypoglycaemia were independently associated with testing behaviour, whereas the presence of late diabetic complications was not. Lower HbA1c was associated with more frequent testing. CONCLUSION: Patient compliance regarding SMBG is limited. Thus, almost two thirds of the patients...

  15. Scale Space Methods for Analysis of Type 2 Diabetes Patients' Blood Glucose Values

    Directory of Open Access Journals (Sweden)

    Stein Olav Skrøvseth

    2011-01-01

    Full Text Available We describe how scale space methods can be used for quantitative analysis of blood glucose concentrations from type 2 diabetes patients. Blood glucose values were recorded voluntarily by the patients over one full year as part of a self-management process, where the time and frequency of the recordings are decided by the patients. This makes a unique dataset in its extent, though with a large variation in reliability of the recordings. Scale space and frequency space techniques are suited to reveal important features of unevenly sampled data, and useful for identifying medically relevant features for use both by patients as part of their self-management process, and provide useful information for physicians.

  16. Scale space methods for analysis of type 2 diabetes patients' blood glucose values.

    Science.gov (United States)

    Skrøvseth, Stein Olav; Godtliebsen, Fred

    2011-01-01

    We describe how scale space methods can be used for quantitative analysis of blood glucose concentrations from type 2 diabetes patients. Blood glucose values were recorded voluntarily by the patients over one full year as part of a self-management process, where the time and frequency of the recordings are decided by the patients. This makes a unique dataset in its extent, though with a large variation in reliability of the recordings. Scale space and frequency space techniques are suited to reveal important features of unevenly sampled data, and useful for identifying medically relevant features for use both by patients as part of their self-management process, and provide useful information for physicians. PMID:21436873

  17. Serum leptin levels correlation with high blood pressure in adult females

    International Nuclear Information System (INIS)

    To measure serum leptin levels and compare them in lean and obese subjects and to identify correlation between serum leptin levels, heart rate and hypertension in lean and obese subjects among adult females. Seventy female subjects with different body mass indices were selected from OPD of Jinnah Medical and Dental College Hospital (OPD), Karachi. Heart rate was counted manually; blood pressure was measured by mercury sphygmomanometer while serum leptin was measured using enzyme-linked immunoassay. The outcomes hypertension and heart rate were correlated to risk factor leptin. Mean heart rate, systolic and diastolic blood pressure and serum leptin levels of obese people were 90+-1, 142+-2, 89+-1 and 24.13+-1.7 respectively, which were significantly higher as compared to lean subjects (p<0.05). All the parameters correlated positively and significantly with increasing BMI. There was a relationship of tachycardia and hypertension with high serum leptin levels in obesity. Serum leptin levels increase with the level of obesity. Hyper-leptinemia is associated with tachycardia and increases in both systolic and diastolic blood pressure in obesity via complex mechanisms. (author)

  18. INTERNET-BASED CONTINGENCY MANAGEMENT TO IMPROVE ADHERENCE WITH BLOOD GLUCOSE TESTING RECOMMENDATIONS FOR TEENS WITH TYPE 1 DIABETES

    OpenAIRE

    Raiff, Bethany R.; Dallery, Jesse

    2010-01-01

    The current study used Internet-based contingency management (CM) to increase adherence with blood glucose testing to at least 4 times daily. Four teens diagnosed with Type 1 diabetes earned vouchers for submitting blood glucose testing videos over a Web site. Participants submitted a mean of 1.7 and 3.1 blood glucose tests per day during the 2 baseline conditions, respectively, compared to 5.7 tests per day during the intervention. Participants and their guardians rated the program favorably...

  19. Blood Flow and Glucose Metabolism in Stage IV Breast Cancer: Heterogeneity of Response During Chemotherapy

    OpenAIRE

    Krak, Nanda; Hoeven, John; Hoekstra, Otto; Twisk, Jos; Wall, Ernst; Lammertsma, A. A.

    2008-01-01

    textabstractObjective: The purpose of the study was to compare early changes in blood flow (BF) and glucose metabolism (MRglu) in metastatic breast cancer lesions of patients treated with chemotherapy. Methods: Eleven women with stage IV cancer and lesions in breast, lymph nodes, liver, and bone were scanned before treatment and after the first course of chemotherapy. BF, distribution volume of water (Vd), MRglu/BF ratio, MRgluand its corresponding rate constants K1and k3were compared per tum...

  20. Poorly Regulated Blood Glucose in Diabetic Patients–predictor of Acute Infections

    OpenAIRE

    Burekovic, Azra; Dizdarevic–Bostandzic, Amela; Godinjak, Amina

    2014-01-01

    Introduction: Diabetes mellitus, the most frequent endocrinology disease is a predisposing factor for infections. Diabetic patients have 4,4 times greater risk of systemic infection than non diabetics. Aim: a) To determine the prevalence and characteristics of acute infectious diseases in hospitalized diabetics; b) To correlate values of blood glucose levels and HbA1c with acute infections in hospitalized diabetics; c) To identify the etiology of infectious diseases. Material and methods: The...

  1. Non-invasive detection of fasting blood glucose level via electrochemical measurement of saliva

    OpenAIRE

    Malik, Sarul; Khadgawat, Rajesh; Anand, Sneh; Gupta, Shalini

    2016-01-01

    Machine learning techniques such as logistic regression (LR), support vector machine (SVM) and artificial neural network (ANN) were used to detect fasting blood glucose levels (FBGL) in a mixed population of healthy and diseased individuals in an Indian population. The occurrence of elevated FBGL was estimated in a non-invasive manner from the status of an individual’s salivary electrochemical parameters such as pH, redox potential, conductivity and concentration of sodium, potassium and calc...

  2. Effect of Different Isoenergetic Breakfast Compositions on Blood Glucose Regulation, Energy Allocation and Satiety

    Directory of Open Access Journals (Sweden)

    Aloys Berg

    2014-08-01

    Full Text Available "Background and purpose: The increasing prevalence of overweight and obesity among adults, demands improved dietary strategies for weight management and metabolic competence. Hence, the objective of this study was to assess the short-term effects of breakfasts with varying macronutrient composition on blood glucose regulation, energy metabolism and satiety. Methods: This study examined ten healthy males (25.6 ± 4.4 yrs; BMI 23.2 ± 0.9 kg/m2 fed isoenergetic breakfasts rich in either Carbohydrate [CH] (68% of energy, Fat [Fat] (64% of energy or Protein [P] (35% of energy or a breakfast which reflected the individuals Normal [N] breakfast composition. Blood glucose and lactate, resting oxygen consumption (VO2, Respiratory Quotient (RQ and satiety feeling were measured. All breakfasts with the exception of the individual normal breakfast variant were isoenergetic and all contained the same amount of dietary fiber. As a non-dietary control, subjects drank 200 ml water on one test day, with the same metabolic parameters measured. Results: Compared with the water control day, there was a significant macronutrient-induced change in the metabolic parameters. The most significant increases in blood glucose were found after the Carbohydrate breakfast and the individual normal breakfast, whereas the Fat and Protein-rich breakfasts induced comparatively smaller blood glucose responses. Only the Proteinrich breakfast led to significant increases in resting VO2 (up to 30% without changes in RQ. Finally, the Protein-rich breakfast induced the highest satiety feeling. Conclusions: Although the Protein-induced effects may initially appear minor, the combination of a reduced glycemic response, increased VO2, a proportionately high fat oxidation and a stronger satiety effect may support the use of this dietary approach for healthy weight management in normal weight men."

  3. Effects of Yogic Exercises on Life Stress and Blood Glucose Levels in Nursing Students

    OpenAIRE

    Kim, Sang Dol

    2014-01-01

    [Purpose] This study was performed to investigate the effects of yogic exercises on life stress and blood glucose levels in nursing students. [Subjects and Methods] The study was a randomized controlled trial. Twenty-seven undergraduate nursing students were randomly selected, with 12 assigned to an exercise group and 15 assigned to a control group. The yogic exercises intervention was undertaken for 60 minutes one day a week for 12 weeks. It consisted of physical exercise (surya namaskara) c...

  4. Diabetes Automata For Diabetes-Related Applications: Software Engine For Blood Glucose Level Simulation

    OpenAIRE

    Agafonov, Aleksandr

    2015-01-01

    Diabetes Automata is a try of concept in the complex research-field of blood glucose simulation and prediction in experimental medical informatics, an experimental research project in software engineering combined with experimental health science. The project integrates together topics such as software system design and development, object-oriented programming, mobile application development, experimental health informatics and electronic health in general and mobile health in particular, and...

  5. Impact of admission blood glucose level on outcomes in community-acquired pneumonia in older adults

    OpenAIRE

    Bhattacharya RK; Mahnken JD; Rigler SK

    2013-01-01

    Rajib K Bhattacharya, Jonathan D Mahnken, Sally K RiglerUniversity of Kansas School of Medicine, Kansas City, KS, USABackground: Community-acquired pneumonia (CAP) is a common cause of morbidity and mortality in older adults. Although diabetes mellitus is a risk factor for pneumonia, the clinical impact of blood glucose level at the time of admission is not clear. Our goal was to examine the association between admission hyperglycemia and subsequent mortality, length of stay, and readmission ...

  6. Blood glucose may be an alternative to cholesterol in CVD risk prediction charts

    OpenAIRE

    Braun, Julia; Bopp, Matthias; Faeh, David

    2013-01-01

    Background Established risk models for the prediction of cardiovascular disease (CVD) include blood pressure, smoking and cholesterol parameters. The use of total cholesterol for CVD risk prediction has been questioned, particularly for primary prevention. We evaluated whether glucose could be used instead of total cholesterol for prediction of fatal CVD using data with long follow-up. Methods We followed-up 6,095 men and women aged ≥16 years who participated 1977-79 in a community based heal...

  7. Do Currently Available Blood Glucose Monitors Meet Regulatory Standards? 1-Day Public Meeting in Arlington, Virginia

    OpenAIRE

    Klonoff, David C.; Reyes, Juliet S.

    2013-01-01

    Blood glucose monitors (BGMs) are approved by regulatory agencies based on their performance during strict testing conducted by their manufacturers. However, after approval, there is uncertainty whether BGMs maintain the accuracy levels that were achieved in the initial data. The availability of inaccurate BGM systems pose a public health problem because their readings serve as a basis for treatment decisions that can be incorrect. Several articles have concluded that BGMs in the marketplace ...

  8. Aging, Vascular Risk and Cognition: Blood Glucose, Pulse Pressure, and Cognitive Performance in Healthy Adults

    OpenAIRE

    Dahle, Cheryl L.; Jacobs, Bradley S.; Raz, Naftali

    2009-01-01

    Advanced age is associated with decline in many areas of cognition as well as increased frequency of vascular disease. Well-described risk factors for vascular disease such as diabetes and arterial hypertension have been linked to cognitive deficits beyond those associated with aging. To examine whether vascular health indices such as fasting blood glucose levels and arterial pulse pressure can predict subtle deficits in age-sensitive abilities, we studied 104 healthy adults (age 18 to 78 yea...

  9. What is the optimal blood glucose target in critically ill patients? A nested cohort study

    OpenAIRE

    Ashraf Al-Tarifi; Nabil Abou-Shala; Tamim, Hani M; Rishu, Asgar H.; Yaseen M. Arabi

    2011-01-01

    Aims: There is an uncertainty about what constitutes an optimal level of blood glucose (BG) in critically ill patients. The objective of this study is to identify the optimal BG target for glycemic control in critically ill patients that is associated with survival benefit with the least hypoglycemia risk. Setting and Design: This is a nested cohort study within a randomized control trial conducted in a tertiary care center in King Abdulaziz Medical City, Riyadh, Kingdom of Saudi Arabia. ...

  10. Dioscorea alata L. Reduces Body Weight by Reducing Food Intake and Fasting Blood Glucose Level

    OpenAIRE

    Helen, Olubobokun Titilope; Olusola, Aluko Esther; Eghosa E, Iyare; Bond, Anyaehie Ugochukwu

    2013-01-01

    Aims: The prevalence of type 2 diabetes may be most likely linked to obesity and its prevalence appears to have increased as the prevalence of obesity increased. Dioscorea alata L. has been observed to possess flavonoids which induce antidiabetic effect and phenolic compounds which could help in weight management. It has been postulated that any therapeutic regimes that can limit weight gain while simultaneously controlling blood glucose levels will be effective in managing diabetes. This stu...

  11. Low perfusion index affects the difference in glucose level between capillary and venous blood

    OpenAIRE

    Acar N; Ozcelik H; Cevik AA; Ozakin E; Yorulmaz G; Kebapci N; Bilge U; Bilgin M

    2014-01-01

    Nurdan Acar,1 Hamit Ozcelik,1 Arif Alper Cevik,1 Engin Ozakin,1 Goknur Yorulmaz,2 Nur Kebapci,2 Ugur Bilge,3 Muzaffer Bilgin4 1Emergency Department, 2Endocrinology Department, 3Family Medicine Department, 4Biostatistics Department, Medical School, Eskisehir Osmangazi University, Eskisehir, Turkey Aim: In emergency cases, finger stick testing is primarily used to check the blood glucose value of patients since it takes longer to obtain the venous value. In critical patients, under conditions ...

  12. Diabetes screening and the distribution of blood glucose levels in rural areas of North India

    OpenAIRE

    Tomi Thomas; Shalini Prabhata; Sameer Valsangkar

    2015-01-01

    Background and Objectives: Genetics and environment have both been implicated in the exponential rise in the prevalence of diabetes mellitus that affects 65.1 million people, and leads to a mortality of 1 million people every year in India. This study was devised to obtain the trends of the distribution of blood glucose, and sociodemographic characteristics in rural areas of a North Indian state. Materials and Methods: A cross-sectional study was conducted at eight centers in five districts. ...

  13. Blood glucose regulation in diabetics. A flatness based nonlinear control simulation study

    Science.gov (United States)

    Cocha, Guillermo; Podestá, Melina; Mazzadi, Alejandro; Amorena, Carlos; D’Atellis, Carlos

    2016-04-01

    Flat systems are a generalization of linear systems, but the techniques used for controlling flat systems are much different than many of the existing techniques for linear systems. In this paper we present the flatness-based control of blood glucose regulation in human system. A non-near model, he Bergman Minimal Model, is used o represent he dynamics of blood regulation in humans and because of the flatness property, he system variables can be expressed as functions of he at output and heir time derivatives and a control aw developed.

  14. Chromium and cobalt ion concentrations in blood and serum following various types of metal-on-metal hip arthroplasties

    DEFF Research Database (Denmark)

    Jantzen, Christopher; Jørgensen, Henrik L; Duus, Benn R;

    2013-01-01

    Widely different metal ion concentrations in blood and serum have been reported with metal-on-metal (MoM) implants. We reviewed the literature on blood and serum ion concentrations of chromium (Cr) and cobalt (Co) following various MoM hip arthroplasties.......Widely different metal ion concentrations in blood and serum have been reported with metal-on-metal (MoM) implants. We reviewed the literature on blood and serum ion concentrations of chromium (Cr) and cobalt (Co) following various MoM hip arthroplasties....

  15. Radioimmunological determination of parathyroid normal concentration in the blood serum

    International Nuclear Information System (INIS)

    The radioimmunological method was used for determining the concentration of parathyroid hormone (PTH) in the serum. A comparative study was made of its two modifications with the use of the heterological systems ''antigen - antibody''. The hormone content of parathyroid glands was studied in practically healthy adults and in children at an age of 9-10 years. It was shown that the level of PTH in adults was 0.46+-0,04 ng/ml (in the set of the firm CIS) and 0.32+-0.18 ng/ml (in the set of the firm DRG). The concentration of PTH in children of the group studied was 1.00+-0.11 ng/ml (in the French set). Reproducibility of the method was studied

  16. Effects of laser acupoint irradiation on blood glucose and glycosylated hemoglobin in type 2 diabetes mellitus

    Science.gov (United States)

    Hui-Hui, Liu; Guo-Xin, Xiong; Li-Ping, Zhang

    2016-06-01

    To investigate the effects of semiconductor laser acupoint irradiation on blood glucose, glycosylated hemoglobin and physical fitness in type 2 diabetes mellitus, 44 cases of type 2 diabetic patients were randomly divided into a control group and a treatment group. All patients in both groups were given a drug treatment. The Hegu, Quchi and Zusanli acupoints of patients in the treatment group were then irradiated daily for 15 d with a 10 MW semiconductor laser. Before and after treatment, patients in both groups underwent a variety of tests and measurements: a two-hour postprandial blood glucose test; a glycosylated hemoglobin test and body mass index (BMI), waist-to-hip ratio (WHR) and body fat percentage (BFP) measurements. The data detected after treatment greatly decreased in the treatment group and was significantly different from that in the control group. It is shown that the acupoint irradiation with a semiconductor laser can improve two-hour postprandial blood glucose, glycosylated hemoglobin and some physical fitness measurements in type 2 diabetes mellitus patients.

  17. System Accuracy Evaluation of the GlucoRx Nexus Voice TD-4280 Blood Glucose Monitoring System

    Directory of Open Access Journals (Sweden)

    Muhammad Khan

    2014-01-01

    Full Text Available Use of blood glucose (BG meters in the self-monitoring of blood glucose (SMBG significantly lowers the risk of diabetic complications. With several BG meters now commercially available, the International Organization for Standardization (ISO ensures that each BG meter conforms to a set degree of accuracy. Although adherence to ISO guidelines is a prerequisite for commercialization in Europe, several BG meters claim to meet the ISO guidelines yet fail to do so on internal validation. We conducted a study to determine whether the accuracy of the GlucoRx Nexus TD-4280 meter, utilized by our department for its cost-effectiveness, complied with ISO guidelines. 105 patients requiring laboratory blood glucose analysis were randomly selected and reference measurements were determined by the UniCel DxC 800 clinical system. Overall the BG meter failed to adhere to the ≥95% accuracy criterion required by both the 15197:2003 (overall accuracy 92.4% and 15197:2013 protocol (overall accuracy 86.7%. Inaccurate meters have an inherent risk of over- and/or underestimating the true BG concentration, thereby risking patients to incorrect therapeutic interventions. Our study demonstrates the importance of internally validating the accuracy of BG meters to ensure that its accuracy is accepted by standardized guidelines.

  18. A Soxhlet Extract of Gongronema latifolium Retains Moderate Blood Glucose Lowering Effect and Produces Structural Recovery in the Pancreas of STZ-Induced Diabetic Rats

    Directory of Open Access Journals (Sweden)

    Bassel Al-Hindi

    2016-04-01

    Full Text Available Background: Gongronema latifolium Benth. (GL possesses considerable glucose lowering effects able to be utilized on a large-scale. This paper investigates the effects of a Soxhlet extract on hyperglycemia, Langerhans islets and glucose uptake by abdominal muscles. Methods: Ethanol and a Soxhlet apparatus were used to obtain GL ethanolic Soxhlet extract (GLES. It was then administered to randomly-segregated male Sprague-Dawley, normal and STZ-induced diabetic rats, using oral gavage to evaluate blood glucose levels (BGLs, serum lipid profile, insulin levels and the pancreas post-treatment. Results: GLES significantly (p < 0.05 decreased BGLs of normal rats in glucose tolerance testing at a dose of 2 g/kg b.w. but failed to do so in diabetic rats undergoing acute 7-h treatment. Given twice-daily, 1 g/kg b.w. of GLES moderately controlled diabetic BGLs starting from day 10. After 14 days of treatment, 1 g/kg and 0.5 g/kg b.w. of GLES caused 44% and 50% respective increases in the average area of Langerhans islets compared to DC. Using isolated rat abdominal muscle, GLES was found to be a mild insulin-sensitizer. GC-MS analysis revealed the presence of the known glucose-lowering phytosterol, Sitostenone. Conclusion: Despite retaining moderate antidiabetic activity, Soxhlet extraction of Gongronema latifolium probably leads to the destruction of active heat-liable compounds.

  19. Endosperm and whole grain rye breads are characterized by low post-prandial insulin response and a beneficial blood glucose profile

    Directory of Open Access Journals (Sweden)

    Östman Elin M

    2009-09-01

    Full Text Available Abstract Background Rye products have previously been shown to induce comparatively low post-prandial insulin responses; irrespectively of their glycaemic indices (GI. However, the mechanism behind this lowered insulin demand remains unknown. An improved insulin economy might contribute to the benefits seen in epidemiological studies with whole grain diets on metabolic risk factors and weight regulation. The objective of this study was to explore the mechanism for a reduced post-prandial insulin demand with rye products. Methods 12 healthy subjects were given flour based rye products made from endosperm, whole grain or bran, produced with different methods (baking, simulated sour-dough baking and boiling as breakfasts in random order in a cross-over design. White wheat bread (WWB was used as a reference. Blood glucose, serum insulin, plasma ghrelin and subjective satiety were measured during 180 minutes. To evaluate the course of post-meal glycaemia, a measure of the glycaemic profile (GP was introduced defined as the duration for the incremental post-prandial blood glucose response divided with the blood glucose incremental peak (min/mM. Results The study shows that whole grain rye breads and endosperm rye products induced significantly (p Conclusion Our study shows that endosperm and wholegrain rye products induce low acute insulinaemic responses and improved glycaemic profiles. The results also suggest that the rye products possess beneficial appetite regulating properties. Further studies are needed to identify the unknown property or bioactive component(s responsible for these beneficial metabolic features of rye.

  20. Glucagon-like peptide-1 (GLP-1) raises blood-brain glucose transfer capacity and hexokinase activity in human brain

    DEFF Research Database (Denmark)

    Jensen, Michael Gejl; Lerche, Susanne; Egefjord, Lærke; Brock, Birgitte; Møller, Niels; Vang, Kim; Rodell, Anders B; Bibby, Bo M; Holst, Jens Juul; Rungby, Jørgen; Gjedde, Albert

    2013-01-01

    phosphorylation velocity (V max) in the gray matter regions of cerebral cortex, thalamus, and cerebellum, as well as increased blood-brain glucose transport capacity (T max) in gray matter, white matter, cortex, thalamus, and cerebellum. In hypoglycemia, GLP-1 had no effects on net glucose metabolism, brain......In hyperglycemia, glucagon-like peptide-1 (GLP-1) lowers brain glucose concentration together with increased net blood-brain clearance and brain metabolism, but it is not known whether this effect depends on the prevailing plasma glucose (PG) concentration. In hypoglycemia, glucose depletion...... potentially impairs brain function. Here, we test the hypothesis that GLP-1 exacerbates the effect of hypoglycemia. To test the hypothesis, we determined glucose transport and consumption rates in seven healthy men in a randomized, double-blinded placebo-controlled cross-over experimental design. The acute...

  1. Bifidogenic effect of grain larvae extract on serum lipid, glucose and intestinal microflora in rats

    Indian Academy of Sciences (India)

    Sang-Oh Park; Byung-Sung Park

    2015-09-01

    The main objective of this study was to investigate whether orally administered Korean grain larvae ethanol extract (GLE) had a bifidogenic effect in normal rats. Male Sprague–Dawley rats were divided into a negative control group (CO) and GLE orally administered (5.0, 7.0 and 9.0 mg/100 g body weight) groups. Thymus and spleen weights dosedependently increased by 128.58% and 128.58%, respectively, but abdominal fat decreased by 19.18% after GLE administration compared with that in the CO group (<0.05). Serum triglycerides, total cholesterol, low-density lipoprotein cholesterol, and glucose decreased by 30.26%, 7.33%, 27.20%, and 6.96%, respectively, whereas highdensity lipoprotein cholesterol increased by 129.93% in the GLE groups compared with those in the CO group (<0.05). IgG, IgM, IgA in the GLE groups increased 203.68%, 181.41%, and 238.25%, respectively, compared to that in the CO group (<0.05). Bifidobacteria and Lactobacillus increased by 115.74% and 144.28%, whereas Bacteroides, Clostridium, Escherichia, and Streptococcus decreased by 17.37%, 17.46%, 21.25%, and 19.16%, respectively, in the GLE groups compared with those in the CO group (<0.05). Total organic acids, acetic acid, and propionic acid increased by 151.40%, 188.09%, and 150.17%, whereas butyric acid and valeric acid decreased by 40.65% and 49.24%, respectively, in the GLE groups as compared with those in the CO group (<0.05). These results suggest that Korean GLE improves the bifidogenic effect by increasing cecal organic acids and modulating gut microflora via a selective increase in Bifidobacterium in normal rats.

  2. Prevalence of glucose-6-phosphate dehydrogenase deficiency and sickle cell trait among blood donors in Riyadh

    Directory of Open Access Journals (Sweden)

    Alabdulaali Mohammed

    2010-01-01

    Full Text Available Background and Aims: Blood donation from glucose-6-phosphate dehydrogenase (G6PD-deficient and sickle cell trait (SCT donors might alter the quality of the donated blood during processing, storage or in the recipient′s circulatory system. The aim of this study was to determine the prevalence of G6PD deficiency and SCT among blood donors coming to King Khalid University Hospital (KKUH in Riyadh. It was also reviewed the benefits and risks of transfusing blood from these blood donors. Materials and Methods: This cross-sectional study was conducted on 1150 blood samples obtained from blood donors that presented to KKUH blood bank during the period April 2006 to May 2006. All samples were tested for Hb-S by solubility test, alkaline gel electrophoresis; and for G6PD deficiency, by fluorescent spot test. Results: Out of the 1150 donors, 23 (2% were diagnosed for SCT, 9 (0.78% for G6PD deficiency and 4 (0.35% for both conditions. Our prevalence of SCT and G6PD deficiency is higher than that of the general population of Riyadh. Conclusion: We recommend to screen all units for G6PD deficiency and sickle cell trait and to defer donations from donors with either of these conditions, unless if needed for special blood group compatibility, platelet apheresis or if these are likely to affect the blood bank inventory. If such blood is to be used, special precautions need to be undertaken to avoid complications in high-risk recipients.

  3. Association between maternal diet factors and hemoglobin levels, glucose tolerance, blood pressure and gestational age in a Hispanic population.

    Science.gov (United States)

    Soto, Roxana; Guilloty, Natacha; Anzalota, Liza; Rosario, Zaira; Cordero, José F; Palacios, Cristina

    2015-06-01

    The aim of this study was to describe the dietary patterns of pregnant women in northern Puerto Rico and explore associations between diet factors with pregnancy related measurements. This analysis is based on the Puerto Rico Testsite for Exploring Contamination Threats (PROTECT), a prospective cohort that is studying environmental risk factors for preterm births in PR. Participants completed a food frequency questionnaire (FFQ) around 20-28 weeks of gestation. The following pregnancy related measures were collected from the medical records: hemoglobin, blood glucose, blood pressure and gestational age. Potential associations between diet factors and pregnancy measures were assessed using chi square analysis with SPSS. A total of 180 participants completed the FFQ; low hemoglobin levels was found in 19.2%, high blood glucose levels was found in 21.1% by fasting blood glucose test and 24.6%by 1-hour 50 g oral glucose screening test, high blood pressure was found in 2.9% (systolic) and 6.5% (diastolic), and pre-term birth was found in 10.4% of the participants. High consumption of rice, desserts and sweets was associated with higher levels of fasting blood glucose levels (p desserts, can lead to high levels of blood glucose and can be a potential predictor of other pregnancy complications during pregnancy in these study participants, such as gestational diabetes. PMID:26817380

  4. Simultaneous determination of eight anticoagulant rodenticides in blood serum and liver.

    Science.gov (United States)

    Chalermchaikit, T; Felice, L J; Murphy, M J

    1993-01-01

    A liquid chromatographic method was developed for the analysis of indandione and 4-hydroxycoumarin anticoagulant rodenticides in blood serum and liver. The method enabled the measurement of serum and liver concentrations of eight anticoagulant rodenticides: brodifacoum, bromadiolone, chlorophacinone, coumafuryl, coumatetralyl, diphacinone, difenacoum, and warfarin. Anticoagulants were extracted from serum and liver with acetonitrile. Extracts were applied to solid-phase extraction columns, which contained mixed packings. Column eluates were evaporated to dryness, reconstituted, and subjected to reversed-phase liquid chromatography. Hydroxycoumarins were detected by fluorescence at an excitation wavelength of 318 nm and an emission wavelength of 390 nm. Indandiones were detected by UV absorption at 285 nm. Extraction efficiencies of greater than 75% for serum and greater than 69% for liver were obtained. The within-run precision (CV) ranged from 2.4 to 8.6% for serum and 2.6 to 8.7% for liver. The between-run precision (CV) ranged from 1.5 to 12.2% for serum and from 2.1 to 11.8% for liver. Hydroxycoumarin rodenticides were detected at 1 ng/mL of serum and 1 ng/g of liver. Indandiones were detected at 10 ng/mL of serum and 10 ng/g of liver. PMID:8429630

  5. Blood glucose may be an alternative to cholesterol in CVD risk prediction charts

    Science.gov (United States)

    2013-01-01

    Background Established risk models for the prediction of cardiovascular disease (CVD) include blood pressure, smoking and cholesterol parameters. The use of total cholesterol for CVD risk prediction has been questioned, particularly for primary prevention. We evaluated whether glucose could be used instead of total cholesterol for prediction of fatal CVD using data with long follow-up. Methods We followed-up 6,095 men and women aged ≥16 years who participated 1977-79 in a community based health study and were anonymously linked with the Swiss National Cohort until the end of 2008. During follow-up, 727 participants died of CVD. Based on the ESC SCORE methodology (Weibull regression), we used age, sex, blood pressure, smoking, and fasting glucose or total cholesterol. The mean Brier score (BS), area under the receiver-operating characteristic curve (AUC) and integrated discrimination improvement (IDI) were used for model comparison. We validated our models internally using cross-validation and externally using another data set. Results In our models, the p-value of total cholesterol was 0.046, that of glucose was p < 0.001. The model with glucose had a slightly better predictive capacity (BS: 2216x10-5 vs. 2232x10-5; AUC: 0.9181 vs. 0.9169, IDI: 0.009 with p-value 0.026) and could well discriminate the overall risk of persons with high and low concentrations. The external validation confirmed these findings. Conclusions Our study suggests that instead of total cholesterol glucose can be used in models predicting overall CVD mortality risk. PMID:23351551

  6. Serum contents of endocannabinoids are correlated with blood pressure in depressed women

    Directory of Open Access Journals (Sweden)

    Ho WS Vanessa

    2012-02-01

    Full Text Available Abstract Background Depression is known to be a risk factor for cardiovascular diseases but the underlying mechanisms remain unclear. Since recent preclinical evidence suggests that endogenous agonists of cannabinoid receptors (endocannabinoids are involved in both cardiovascular function and depression, we asked whether endocannabinoids correlated with either in humans. Results Resting blood pressure and serum content of endocannabinoids in ambulatory, medication-free, female volunteers with depression (n = 28 and their age- and ethnicity-matched controls (n = 27 were measured. In females with depression, both diastolic and mean arterial blood pressures were positively correlated with serum contents of the endocannabinoids, N-arachidonylethanolamine (anandamide and 2-arachidonoylglycerol. There was no correlation between blood pressure and endocannabinoids in control subjects. Furthermore, depressed women had significantly higher systolic blood pressure than control subjects. A larger body mass index was also found in depressed women, however, it was not significantly correlated with serum endocannabinoid contents. Conclusions This preliminary study raises the possibility that endocannabinoids play a role in blood pressure regulation in depressives with higher blood pressure, and suggests an interrelationship among endocannabinoids, depression and cardiovascular risk factors in women.

  7. Performance of two updated blood glucose monitoring systems: an evaluation following ISO 15197:2013.

    Science.gov (United States)

    Pleus, Stefan; Baumstark, Annette; Rittmeyer, Delia; Jendrike, Nina; Haug, Cornelia; Freckmann, Guido

    2016-05-01

    Objective For patients with diabetes, regular self-monitoring of blood glucose (SMBG) is essential to ensure adequate glycemic control. Therefore, accurate and reliable blood glucose measurements with SMBG systems are necessary. The international standard ISO 15197 describes requirements for SMBG systems, such as limits within which 95% of glucose results have to fall to reach acceptable system accuracy. The 2013 version of this standard sets higher demands, especially regarding system accuracy, than the currently still valid edition. ISO 15197 can be applied by manufacturers to receive a CE mark for their system. Research design and methods This study was an accuracy evaluation following ISO 15197:2013 section 6.3 of two recently updated SMBG systems (Contour * and Contour TS; Bayer Consumer Care AG, Basel, Switzerland) with an improved algorithm to investigate whether the systems fulfill the requirements of the new standard. For this purpose, capillary blood samples of approximately 100 participants were measured with three test strip lots of both systems and deviations from glucose values obtained with a hexokinase-based comparison method (Cobas Integra † 400 plus; Roche Instrument Center, Rotkreuz, Switzerland) were determined. Percentages of values within the acceptance criteria of ISO 15197:2013 were calculated. This study was registered at clinicaltrials.gov (NCT02358408). Main outcome Both updated systems fulfilled the system accuracy requirements of ISO 15197:2013 as 98.5% to 100% of the results were within the stipulated limits. Furthermore, all results were within the clinically non-critical zones A and B of the consensus error grid for type 1 diabetes. Conclusions The technical improvement of the systems ensured compliance with ISO 15197 in the hands of healthcare professionals even in its more stringent 2013 version. Alternative presentation of system accuracy results in radar plots provides additional information with certain advantages. In addition

  8. Simultaneous measurement of blood flow and glucose metabolism by autoradiographic techniques

    Energy Technology Data Exchange (ETDEWEB)

    Mies, G.; Niebuhr, I.; Hossmann, K.A.

    A double tracer autoradiographic technique using 131I-iodo-antipyrine and 14C-deoxyglucose is presented for the simultaneous measurement of blood flow and cerebral glucose utilization in the same animal. 131I is a gamma emitting isotope with a half life of 8.06 days and can be detected with adequate resolution on standard autoradiographic films. Autoradiograms are made before and after decay of 131I; the time interval between the 2 exposures and the concentration of the 2 tracers is adjusted to avoid significant cross-contamination. In this way, 2 film exposures are obtained which can be processed quantitatively like single tracer autoradiograms. The validity of the method for the investigation of local coupling of flow and metabolism was tested under various physiological and pathophysiological conditions. Coupling was tight in barbiturate-anesthetized healthy animals, but not under halothane anesthesia where uncoupling occurred in various subcortical structures. Focal seizures induced by topical application of penicillin on the cortical surface led to a coupled increase of metabolism and flow in thalamic relay nuclei but not at the site of penicillin administration where increased glucose utilization was not accompanied by similar increase in blood flow. Both coupled and uncoupled increases in local glucose utilization were observed in spreading depression and in circumscribed areas of experimental brain tumors. The results obtained demonstrate that double tracer autoradiography allows allows the very precise local assessment of cerebral blood flow and glucose utilization, and, therefore, is particularly suited to the study of regional coupling processes under various experimental conditions.

  9. Simultaneous measurement of blood flow and glucose metabolism by autoradiographic techniques

    International Nuclear Information System (INIS)

    A double tracer autoradiographic technique using 131I-iodo-antipyrine and 14C-deoxyglucose is presented for the simultaneous measurement of blood flow and cerebral glucose utilization in the same animal. 131I is a gamma emitting isotope with a half life of 8.06 days and can be detected with adequate resolution on standard autoradiographic films. Autoradiograms are made before and after decay of 131I; the time interval between the 2 exposures and the concentration of the 2 tracers is adjusted to avoid significant cross-contamination. In this way, 2 film exposures are obtained which can be processed quantitatively like single tracer autoradiograms. The validity of the method for the investigation of local coupling of flow and metabolism was tested under various physiological and pathophysiological conditions. Coupling was tight in barbiturate-anesthetized healthy animals, but not under halothane anesthesia where uncoupling occurred in various subcortical structures. Focal seizures induced by topical application of penicillin on the cortical surface led to a coupled increase of metabolism and flow in thalamic relay nuclei but not at the site of penicillin administration where increased glucose utilization was not accompanied by similar increase in blood flow. Both coupled and uncoupled increases in local glucose utilization were observed in spreading depression and in circumscribed areas of experimental brain tumors. The results obtained demonstrate that double tracer autoradiography allows allows the very precise local assessment of cerebral blood flow and glucose utilization, and, therefore, is particularly suited to the study of regional coupling processes under various experimental conditions

  10. Determination of selenium in blood serum by inductively coupled plasma atomic emission spectrometry with pneumatic nebulization

    Energy Technology Data Exchange (ETDEWEB)

    Machat, Jiri; Kanicky, Viktor; Otruba, Vitezslav [Laboratory of Plasma Sources for Chemical Analysis-Laboratory of Atomic Spectrochemistry, Faculty of Science, Masaryk University Brno (Czech Republic)

    2002-02-01

    The possibility of determining selenium in blood serum using inductively coupled plasma emission spectrometry with conventional pneumatic nebulization was studied. A high-resolution spectrometer (SBW=6 pm) with laterally viewed ICP was employed. Analysis with conventional pneumatic nebulization could overcome laborious and demanding digestion, which is necessary for hydride generation. A pressure digestion with nitric acid at 160 C was sufficient to decrease the carbon content in the serum sample to 5%-10% of its original value. Spectral interference of the CN band was observed and mathematically corrected. It was found that the carbon-induced selenium line emission enhancement occurred even under ICP optimized conditions. A method of determination was developed and applied to the analysis of blood serum. True limit of detection in real samples is 0.01-0.02 mg/L and the limit of quantification (RSD 10%) is 0.03-0.07 mg/L using Se I 196.090 nm line at an integration time of 10-2 s. The method was tested by analysis of porcine blood serum and the serum reference material Seronorm MI 0181. (orig.)

  11. Impaired first-phase insulin response predicts postprandial blood glucose increment in patients with recently diagnosed type 2 diabetes

    DEFF Research Database (Denmark)

    Gredal, C; Rosenfalck, A M; Dejgaard, Anders;

    2007-01-01

    The aim of the study was to evaluate the relationship between postprandial blood glucose and first-phase insulin response and, furthermore, to assess whether the intravenous glucagon stimulation test can be used as a predictor for increased postprandial glucose in patients with recently diagnosed...... type 2 diabetes....

  12. Effect of glucose on photoacoustic signals at the wavelengths of 1064 and 532 nm in pig blood and intralipid

    International Nuclear Information System (INIS)

    Non-invasive glucose monitoring is one of the most active areas in biomedical research. Various techniques have been developed over the years to meet the clinical requirements of non-invasive monitoring in the physiologically relevant glucose concentration range, but without a breakthrough. This paper used the pulsed photoacoustic (PA) technique to study glucose-induced changes in pig whole blood and 1% IntralipidTM using an Nd : YAG laser with wavelengths of 1064 and 532 nm as the optical energy source. Scattering properties of the sample significantly affect the laser-induced pressure waves. Glucose was found to affect both the scattering and the absorption properties of the samples. The results showed an increase of 11.4%/500 mg dl-1 added glucose in the peak-to-peak value of the PA signal in blood at 1064 nm, whereas the corresponding increase was only 1.35%/500 mg dl-1 in 1% IntralipidTM. At a wavelength of 532 nm, the glucose increased the peak-to-peak value of the PA signal by 6.0%/500 mg dl-1 added glucose in blood. On the whole, the pulsed PA technique proved to be an efficient tool for the study of glucose-induced changes in blood and tissue phantoms in vitro

  13. A Comparative Study of Blood Glucose Measurements Using Glucometer Readings and the Standard Method in the Diagnosis of Neonatal Hypoglycemia

    Directory of Open Access Journals (Sweden)

    Mohammad Torkaman

    2016-03-01

    Full Text Available Background: Hypoglycemia is one of the most common neonatal disorders, associated with severe complications. There has been a great deal of controversy regarding the definition and screening of hypoglycemia. Therefore, in this study, we aimed to determine a cut-off value for blood glucose level in glucometer readings. Methods: This cross-sectional study was conducted on 238 newborns at risk of hypoglycemia, admitted to Baqiyatallah Hospital of Tehran, Iran in 2012; the subjects were selected via simple sampling. After obtaining informed consents from the newborns’ parents, 1 cc blood samples were sent to the laboratory for measuring the blood glucose level. Moreover, venous blood samples, as well as heel-stick blood samples, were obtained for glucometer measurements. Blood glucose measurements were used to determine the cut-off value by the receiver operating characteristic (ROC curve and make comparisons with the diagnostic criteria for hypoglycemia in the literature. Results: A total of 238 infants with the mean weight of 2869±821.9 g were enrolled in this study. The mean (±SD blood glucose levels were 65.1±22.9, 82.9±24.7, and 84.4±24.8 mg/dl, based on the standard laboratory method, glucometer reading of venous blood samples, and glucometer reading of heel-stick capillary blood samples, respectively. The optimal cut-off point for hypoglycemia was determined as 65 mg/dl, using glucometer-based assessment of heel-stick blood samples. Conclusion: The significant difference in blood glucose levels measured by the laboratory method and outpatient glucometer readings highlights the importance of a cut-off value for rapid assessment and control of blood glucose and timely detection of hypoglycemia. In fact, the cut-off value introduced in the present study could facilitate such measurements.

  14. An enzymatic glucose/O2 biofuel cell operating in human blood.

    Science.gov (United States)

    Cadet, Marine; Gounel, Sébastien; Stines-Chaumeil, Claire; Brilland, Xavier; Rouhana, Jad; Louerat, Frédéric; Mano, Nicolas

    2016-09-15

    Enzymatic biofuel cells (BFCs) may power implanted medical devices and will rely on the use of glucose and O2 available in human bodily fluids. Other than well-established experiments in aqueous buffer, little work has been performed in whole human blood because it contains numerous inhibiting molecules. Here, we tested our BFCs in 30 anonymized, random and disease-free whole human blood samples. We show that by designing our anodic and cathodic bioelectrocatalysts with osmium based redox polymers and home-made enzymes we could reach a high selectivity and biofunctionnality. After optimization, BFCs generate power densities directly proportional to the glycaemia of human blood and reached a maximum power density of 129µWcm(-2) at 0.38V vs. Ag/AgCl at 8.22mM glucose. This is to our knowledge the highest power density attained so far in human blood and open the way for the powering of integrated medical feedback loops. PMID:27107143

  15. The Effect of Abelmoschus Esculentus on Blood Levels of Glucose in Diabetes Mellitus

    Science.gov (United States)

    Khosrozadeh, Maryam; Heydari, Naval; Abootalebi, Malihe

    2016-01-01

    Background: Diabetes mellitus is a metabolic disorder that results in hyperglycemia. According to the statistics of the International Diabetes Federation (IDF), this problem has a fast growing prevalence and, unfortunately, leaves permanent complications on different body systems. For this reasons, nowadays attentions has been paid to the traditional medicine such as Okra (Abelmoscus esculentus). The aim of this study was the evaluation of the effect of Okra (Abelmoscus esculentus) on blood levels of glucose in diabetes. Methods: This is a review article, which was obtained by a search in databases such as PubMed, Google Scholar, and Magiran by using keywords such as diabetes, Okra, and hypoglycemic effect. Results: Various studies on Okra (Abelmoscus esculentus) showed that Abelmoscus esculentus (AE)/Okra extract has a hypoglycemic effect that helps decrease blood glucose level. Its properties can be a useful remedy to manage diabetes mellitus. In addition, it leads to inhibition of cholesterol absorption and subsequently decreases the level of lipid and fat in the blood. The results of an investigation on diabetic mice by using this material has shown the same effect and confirmed this conclusion. Conclusion: Based on the positive effects of Okra on reducing blood sugar level, the widespread use of this plant is recommended. Clearly, further research is required.

  16. Identification of Glucose-Binding Pockets in Human Serum Albumin Using Support Vector Machine and Molecular Dynamics Simulations.

    Science.gov (United States)

    Ranganarayanan, Preethi; Thanigesan, Narmadha; Ananth, Vivek; Jayaraman, Valadi K; Ramakrishnan, Vigneshwar

    2016-01-01

    Human Serum Albumin (HSA) has been suggested to be an alternate biomarker to the existing Hemoglobin-A1c (HbA1c) marker for glycemic monitoring. Development and usage of HSA as an alternate biomarker requires the identification of glycation sites, or equivalently, glucose-binding pockets. In this work, we combine molecular dynamics simulations of HSA and the state-of-art machine learning method Support Vector Machine (SVM) to predict glucose-binding pockets in HSA. SVM uses the three dimensional arrangement of atoms and their chemical properties to predict glucose-binding ability of a pocket. Feature selection reveals that the arrangement of atoms and their chemical properties within the first 4Å from the centroid of the pocket play an important role in the binding of glucose. With a 10-fold cross validation accuracy of 84 percent, our SVM model reveals seven new potential glucose-binding sites in HSA of which two are exposed only during the dynamics of HSA. The predictions are further corroborated using docking studies. These findings can complement studies directed towards the development of HSA as an alternate biomarker for glycemic monitoring. PMID:26886739

  17. Glucose Tests

    Science.gov (United States)

    ... be limited. Home Visit Global Sites Search Help? Glucose Tests Share this page: Was this page helpful? ... the meaning of other test results. Fasting Blood Glucose Glucose Level Indication From 70 to 99 mg/ ...

  18. The correlation of the serum leptin, insulin resistance, endocrine hormone, blood lipid and of simple obesity children and growth

    Institute of Scientific and Technical Information of China (English)

    Xiao-Yu Wang; Xiao-Yun Liu; Kai-Yu Xu

    2015-01-01

    Objective:To analyze the correlation of the serum leptin, insulin resistance, Endocrine hormone, blood lipid and of simple obesity children and growth.Methods:200 cases of simple obesity children (Study Group) and 200 cases of non obese children (control group) were studied, Fasting blood glucose(FBG), Glycosylated hemoglobin (HbAlc), the serum leptin detection (Leptin), insulin (INS), insulin resistance index (HOMA2-IR), growth hormone (GH), cortisol (cortisol), thyroid hormones and blood lipid indexes were detected.Results:Leptin, cortisol and T3 levels of study group were (21.39±2.29) g/L, (18.43±1.72) g/mL and (20.71±1.82) pmol/L, were significantly increased compared with the control group, and GH level was (3.15±0.41) g/L, was significantly reduced, the difference of all these indexes were statistically significant in two groups (P0.05), HbAlc, INS and HOMA2 - IR were respectively (5.82±0.87)%, (33.02-2.34) mIU/L, (3.31-0.54), compared with the control group obviously increase than the corresponding numerical of the control group, the difference of all these indexes have statistical significance in two groups (P<0.05). TC, TG and LDL-C of study group were (4.67±0.65) mmol/L, (1.62±0.45) mmol/L and (3.59±0.46) mmol/L, were significantly higher than those in the control group, while HLD-C was (1.10±0.24) mmol/L, was significantly lower (P<0.05). Conclusion:Children with simple obesity have leptin resistance, reduction of growth hormone and endocrine and lipid metabolism disorder, and seriously affect the normal growth and development of children.

  19. Serum selenium concentration in maternal and umbilical cord blood. Relation to course and outcome of pregnancy

    DEFF Research Database (Denmark)

    Bro, S; Berendtsen, H; Nørgaard, J; Høst, A; Jørgensen, P J

    1988-01-01

    The present knowledge of the role of selenium in human fetal and neonatal development is sparse. In this study we measured serum selenium concentrations in maternal and umbilical cord blood from 500 Danish mothers at delivery, looking for a relationship between various maternal and fetal complica...

  20. Quality standards, safety and efficacy of blood-derived serum eye drops: A review.

    Science.gov (United States)

    van der Meer, Pieter F; Seghatchian, Jerard; Marks, Denese C

    2016-02-01

    Serum eye drops (SEDs) are being used increasingly to treat dry eye syndrome and persistent corneal epithelial defects, and are usually prescribed when conventional treatments fail. SEDs are commonly sourced from the patient's own blood via an autologous collection. Although SEDs are clearly beneficial, they are not available for those patients that cannot donate sufficient blood, and some centres are moving to allogeneic SEDs. Many studies have reported that both allogeneic and autologous SEDs are effective. However, few large randomised controlled trials have been conducted to date, and clinical evidence is therefore limited to smaller studies. Alternatives to serum are also being explored, such as platelet lysate and products made from platelet rich plasma, as they are a rich source of growth factors. This article reviews how some centres are approaching allogeneic collections for SEDs, and alternatives to serum that are currently being explored. PMID:26847866

  1. Analysis Article on the Performance Analysis of the OneTouch® UltraVue™ Blood Glucose Monitoring System

    OpenAIRE

    Solnica, Bogdan

    2009-01-01

    In this issue of Journal of Diabetes Science and Technology, Chang and colleagues present the analytical performance evaluation of the OneTouch® UltraVue™ blood glucose meter. This device is an advanced construction with a color display, used-strip ejector, no-button interface, and short assay time. Accuracy studies were performed using a YSI 2300 analyzer, considered the reference. Altogether, 349 pairs of results covering a wide range of blood glucose concentrations were analyzed. Patients ...

  2. Patterns of self-monitoring of blood glucose in insulin-treated diabetes: analysis of a Scottish population over time.

    Science.gov (United States)

    Cameron, D; Harris, F M; Evans, J M M

    2016-07-01

    Analysis of a diabetes clinical information system in Tayside, Scotland, shows that a significant proportion of insulin-treated patients with diabetes are not self-monitoring blood glucose according to current clinical guidance and recommendations, with some not self-monitoring their blood glucose at all. Although there has been an increase in the number of reagent strips dispensed over the past decade, this increase is mainly accounted for by increased testing frequency among people with diabetes already testing. PMID:26990502

  3. Effects of Mad Honey on Blood Glucose and Lipid Levels in Rats With Streptozocin-Induced Diabetes

    OpenAIRE

    ÖZTAŞAN, Nuray

    2005-01-01

    This study investigated whether mad honey affects blood glucose and lipid levels in experimental animals. It was found that mad honey caused significant decreases in blood glucose and lipid levels in animals with streptozocin-induced diabetes mellitus (n = 10) and controls (n = 10). These decreases may be due to grayanotoxins in the mad honey causing the islets of Langerhans in the pancreas to secrete insulin by stimulating the parasympathetic nervous system or M2-muscarinic receptors.

  4. Effects of exposure to electromagnetic field radiation (EMFR) generated by activated mobile phones on fasting blood glucose

    OpenAIRE

    Sultan Ayoub Meo; Khalid Al Rubeaan

    2013-01-01

    Objective: Extensive use of mobile phones has been accompanied by a common public debate about possible adverse effects on human health. No study has been published so far to establish any association between the fastest growing innovation of mobile phone and fasting blood glucose. The aim was to determine the effects of exposure to electromagnetic field radiation generated by mobile phones on fasting blood glucose in Wistar Albino rats. Materials and Methods: 40 Male Albino rats (Wistar Stra...

  5. Blood Glucose as a Predictor of Mortality in Children Admitted to the Hospital with Febrile Illness in Tanzania

    OpenAIRE

    Nadjm, Behzad; Mtove, George; Amos, Ben; Hildenwall, Helena; Najjuka, Anne; Mtei, Frank; Todd, Jim; Reyburn, Hugh

    2013-01-01

    Data from a prospective study of 3,319 children ages 2 months to 5 years admitted with febrile illness to a Tanzanian district hospital were analyzed to determine the relationship of blood glucose and mortality. Hypoglycemia (blood sugar < 2.5 mmol/L and < 45 mg/dL) was found in 105 of 3,319 (3.2%) children at admission, and low-normal blood glucose (2.5-5 mmol/L and 45-90 mg/dL) was found in 773 of 3,319 (23.3%) children. Mortality was inversely related to admission blood sugar; compared wit...

  6. The Effects of 6 Isocaloric Meals Pattern on Blood Lipid Profile, Glucose, Hemoglobin A1c, Insulin and Malondialdehyde in Type 2 Diabetic Patients: A Randomized Clinical Trial

    Directory of Open Access Journals (Sweden)

    Moosa Salehi

    2014-09-01

    Full Text Available Background: The present clinical trial study aims at investigating the effect of daily energy intake in 6 isocaloric meals in comparison with the current meal pattern (3 meals and 2 small snacks per day on type 2 diabetes risk markers in diabetes during 3-month period. Methods: Eighty four type 2 diabetes patients were randomly divided into 6 isocaloric meal diet or a balanced diet (3 meals and 2 snacks previous meal pattern. The planned reduced calorie diets for both groups were identical except for the meal pattern. Blood samples were analyzed before and after the investigation for fasting blood sugar (FBS, two-hour post-prandial glucose (2hPP, insulin, hemoglobin A1c (HbA1c, total cholesterol, triglyceride, HDL-C, LDL-C, and molondialdehyde (MDA concentrations. Results: HbA1c (P=0.00 and body mass index (BMI (P=0.04 values decreased significantly in the 6 isocaloric meal pattern compared with the controls. There were no significant differences in fasting serum glucose (P=0.09, insulin (P=0.65, total cholesterol (P=0.32, LDL-C (P=0.43, HDL-C (P=0.40 cholesterol, triglyceride (P=0.40, MDA (P=0.13 and 2hPP serum glucose (P=0.30 concentrations between the 6 isocaloric meal and tradition meal pattern. Conclusion: Six isocaloric meal pattern in comparison with the current meal pattern led to weight loss and improved glycemic control. Serum lipid profile and MDA did not change significantly. Trial Registration Number: IRCT201205179780N1

  7. Serum glycated albumin is inversely influenced by fat mass and visceral adipose tissue in Chinese with normal glucose tolerance.

    Directory of Open Access Journals (Sweden)

    Feifei Wang

    Full Text Available BACKGROUND: Recent studies have revealed that body mass index (BMI inversely influenced serum glycated albumin (GA, which may cause an underestimation of GA-monitored short-term hyperglycemic control. OBJECTIVE: This study was to investigate the association between anthropometric variables (BMI and waist circumference (W and accurate adiposity variables (percentage of body fat (%fat, fat mass, free fat mass (FFM, subcutaneous fat area (SFA, and visceral fat area (VFA with serum GA. DESIGN: A total of 2563 subjects (1037 men, 593 premenopausal women, and 933 postmenopausal women with normal glucose tolerance underwent bioelectrical impedance body fat content measurement and magnetic resonance imaging. Serum GA and absolute value of GA (aGA were measured by enzymatic assay. RESULTS: Compared to the BMI <25.0 kg/m(2 group, the BMI ≥25.0 kg/m(2 group had significantly higher fasting plasma glucose, glycated hemoglobin A1c, and body fat parameters including W, %fat, fat mass, FFM, SFA, and VFA, but significantly lower aGA, and GA in all the three sex- and menopause-stratified groups (all P<0.05. GA decreased with the increment of fat mass for all three groups (all P for trend <0.001. In the same BMI category, men and postmenopausal women with elevated %fat (men, ≥25%; women, ≥35% still had significantly lower GA than those with normal %fat (men, <25%; women, <35% (all P<0.05. Multiple stepwise regression showed that %fat, fat mass, and VFA were independently associated with GA. CONCLUSIONS: Serum GA was inversely influenced by fat mass and visceral adipose tissue in Chinese with normal glucose tolerance.

  8. Diabetes screening and the distribution of blood glucose levels in rural areas of North India

    Directory of Open Access Journals (Sweden)

    Tomi Thomas

    2015-01-01

    Full Text Available Background and Objectives: Genetics and environment have both been implicated in the exponential rise in the prevalence of diabetes mellitus that affects 65.1 million people, and leads to a mortality of 1 million people every year in India. This study was devised to obtain the trends of the distribution of blood glucose, and sociodemographic characteristics in rural areas of a North Indian state. Materials and Methods: A cross-sectional study was conducted at eight centers in five districts. A camp-based approach was followed in the diabetes screening conducted in rural areas. Blood glucose measurements were obtained after informed consent by trained staff using a reflectance photometer instrument. Descriptive statistics, distribution curves, log transformations, and tests for bimodality were obtained. Results: 45,318 participants consisting of 44.4% males and 55.6% females were screened. Ages ranged from 18 to 98 years with the mean age of 39.9 ± 14.44 years. 86.5% were normal (random blood sugar [RBS] 200 mg/dl. The median blood glucose level steadily rose with increasing age. The prediabetic to diabetic ratio was 4:1. The distribution curve of RBS was right skewed. A log transformation was applied, and bimodality was tested using the Hartigan′s dip test. The dip statistic (D was 0.0162 with a simulated P < 0.001. Conclusion: Mass screening for diabetes provides benefits from a clinical standpoint by helping to estimate the prevalence (diabetes and the hidden burden of the disease (prediabetes. Screening programs can strengthen healthcare system initiatives and reduce the growing burden of diabetes in India.

  9. Blood Transfusion, Serum Ferritin, and Iron in Hemodialysis Patients in Africa

    Directory of Open Access Journals (Sweden)

    Leonard Kouegnigan Rerambiah

    2015-01-01

    Full Text Available Background and Objectives. There is no data analyzing the outcome of blood transfusions and oral iron therapy in patients with kidneys failure in sub-Saharan Africa. The present study aimed to fill that gap and assess the value of ferritin in the diagnosis of iron overload and deficiency. Design. From January to February 2012, we prospectively studied 85 hemodialysis patients (78% of males and 22% of females aged 20 to 79 years attending the Gabonese National Hemodialysis Centre. Results. Correlation studies showed (a a strong positive linear relationship between the number of blood transfusions and high serum ferritin in hemodialysis patient (Spearman r:0.74; P value: 0.0001; (b a weak association between the number of blood transfusions and serum iron concentrations (Spearman r:0.32; P value: 0.04; (c a weak association between serum ferritin and serum iron (Spearman r:0.32; P value: 0.003. Also, the strength of agreement beyond chance between the levels of ferritin and iron in the serum was poor (κ=0.14. The prevalence of iron overload was 10.6%, whereas the prevalence of iron deficiency was 2.3%, comparing (1 patients with a maximum of one transfusion not on iron therapy; (2 patients with a maximum of one transfusion on iron therapy; (3 polytransfused patients not on iron therapy; and (4 polytransfused patients on oral iron therapy. The “Kruskal-Wallis test” showed that ferritin levels varied significantly between the groups (P value: 0.0001. Conclusion. Serum ferritin is not reliable as a marker of iron overload. For patients undergoing regular transfusion we recommend routine serum ferritin measurement and yearly measurement of LIC.

  10. Emotional arousal, blood glucose levels, and memory modulation: three laboratory exercises in cognitive neuroscience.

    Science.gov (United States)

    Flint, Robert W

    2004-01-01

    The relationships between emotional arousal and cognition in humans represent an important field in cognitive neuroscience. Studies examining the characteristics of emotion-induced memory enhancement and the mechanisms through which these effects occur are becoming increasingly common. This article describes three affordable laboratory exercises of relevance to the growing interest in this field. Specifically, Experiment one reviews a protocol for examining memory, hypermnesia, reminiscence, and primacy/recency effects for emotional and neutral words. Experiments two and three provide opportunities to examine the relationships between blood glucose level and memory for either a list of pictures or the spatial location of pictures. Each laboratory exercise contains a certain amount of flexibility and is malleable to the specific needs of the instructor. For example, the use of blood glucose monitoring may be of value to a variety of different exercises examining stress and/or emotional arousal and the stimuli used in each of the protocols may be varied, creating opportunities for a number of different novel exercises. A series of questions have been provided at the end of each exercise in order to help stimulate inclass discussion. The potential application of this line of research in cognitive neuroscience is conveyed through a list of references where glucose has been used to attenuate cognitive deficits in Alzheimer's disease, age-related cognitive decline, and other neuropsychological conditions. PMID:23493939

  11. Low blood glucose levels and other complications during growth hormone supplementation in sepsis

    Directory of Open Access Journals (Sweden)

    Faintuch Joel

    1999-01-01

    Full Text Available Blood glucose levels in the high normal range or even moderate hyperglycemia is the expected profile in septic postoperative patients receiving high-calorie enteral alimentation. The addition of growth hormone as an anabolic agent should additionally reinforce this tendency. In a cancer patient undergoing partial gastrectomy with lymphadenectomy and suffering from postoperative subphrenic abscess and prolonged sepsis, tube feeding (38.3 kcal/kg/day and growth hormone (0.17 IU/kg/day were simultaneously administered for 25 days. Blood glucose levels were in the lower limits of the normal range before growth hormone introduction, and continued with a similar tendency during most of the therapeutic period. Two additional complications, namely heart arrest and peripheral edema, were documented during the same period. It is concluded that sepsis was the most likely mechanism for low glucose values, and that high-calorie enteral diet and growth hormone supplementation did not prevent that result. It is uncertain whether heart arrest was due to the drug, but its association with peripheral edema is well documented in clinical series.

  12. Profile of blood glucose and ultrastucture of beta cells pancreatic islet in alloxan compound induced rats

    Directory of Open Access Journals (Sweden)

    I Nyoman Suarsana

    2010-06-01

    Full Text Available Diabetes is marked by elevated levels of blood glucose, and progressive changes of the structure of pancreatic islet histopathology. The objective of this research was to analyse the glucose level and histophatological feature in pancreatic islet in alloxan compound induced rats. A total of ten male Spraque Dawley rats of 2 months old were used in this study. The rats were divided into two groups: (1 negative control group (K-, and (2 positif induced alloxan group (diabetic group =DM. The rats were induced by a single dose intraperitonial injection of alloxan compound 120 mg/kg of body weight. The treatment was conducted for 28 days. Blood glucose levels of rats were analysed at 0, 4, 7, 14, 21, and 28 days following treatment. At the end of the experiment, rats were sacrificed by cervical dislocation. Pancreas was collected for analysis of histopathological study by Immunohistochemical technique, and ultrastructural study using transmission electron microscope (TEM. The result showed that Langerhans islet of diabetic rat (rat of DM group showed a marked reduction of size, number of Langerhans islet of diabetic rat decrease, and characterized by hyperglycemic condition. By using TEM, beta cells of DM group showed the rupture of mitochondrial membrane, the lost of cisternal structure of inner membrane of mitocondria, reduction of insulin secretory granules, linkage between cells acinar with free Langerhans islet, and the caryopicnotic of nucleus.

  13. Multicomponent determination of 4-hydroxycoumarin anticoagulant rodenticides in blood serum by liquid chromatography with fluorescence detection.

    Science.gov (United States)

    Felice, L J; Chalermchaikit, T; Murphy, M J

    1991-01-01

    A sensitive liquid chromatographic method was developed for the analysis of 4-hydroxycoumarin anticoagulant rodenticides in blood serum. The method can simultaneously measure the serum levels of five anticoagulant rodenticides: brodifacoum, bromadiolone, coumatetralyl, difenacoum, and warfarin. Serum proteins are precipitated with acetonitrile and the supernatant is mixed with ethyl ether. The organic phase is separated, evaporated to dryness, and the residue subjected to chromatographic analysis. The anticoagulants are separated by reversed-phase gradient chromatography with fluorescence detection at an excitation wavelength of 318 nm and emission wavelength of 390 nm. Extraction efficiencies of 68.1 to 98.2% were obtained. The within-run precision (CV) ranged from 2.19 to 3.79% and the between-run precision (CV) from 3.72 to 9.57%. The anticoagulants can be quantitated at serum levels of 10 to 20 ng/mL. PMID:1943055

  14. Concentration of beta-carotene and vitamin A in blood serum of cows in peripartal period

    Directory of Open Access Journals (Sweden)

    Veličković Miljan

    2005-01-01

    Full Text Available In a herd of high-productive dairy cows of the Holstein breed, 40 cows were selected for an experiment. The animals were chosen at the period of advanced gravidity. Data on mean values of beta-carotene concentrations in blood serum of the cows show that significantly higher values (p<0.01 were established in advanced gravidity (x=9.53± 3.49 μmol/l in comparison with the values on calving day (x=5.69±3.14 μmol/l and in early puerperium (x=2.25±1.00 μmol/l. The average concentration of vitamin A in blood serum of cows varied approximately the same as the concentration of beta-carotene. The highest average concentration of vitamin A in blood serum of cows was determined in advanced gravidity (x=1.856±0.52 μmol/l, and the lowest in early puerperium (x=0.988±0.31 μmol/l (p<0.05. It can be concluded on the grounds of the results obtained in this work that the average concentration of beta-carotene and vitamin A in blood serum of cows in advanced gravidity and on the day of calving is within the limits of physiological values and in keeping with the values cited in literature, and that concentrations of beta-carotene and vitamin A in blood serum of cows two weeks after calving are significantly lower than the values obtained in advanced gravidity and on the day of calving and are lower than the physiological values most often reported in literature for this animal specie.

  15. The correlation between serum leptin and blood pressure after exposure to noise at work

    Directory of Open Access Journals (Sweden)

    Muayad S Rahma

    2013-01-01

    Full Text Available Several epidemiologic studies have reported that exposure to noise is associated with cardiovascular disease. The increased body weight is often associated with metabolic as well as increased blood pressure. The aim of this study is to investigate the correlation between the elevation of blood pressure and serum leptin hormones due to the effects of noise in the work place. A total of 80 volunteer males where included in this study with an age range between of 20 and 45 years, they were divided in two groups equally, the 1 st group were exposed to noise in the workplace while the 2 nd group were not. The individual noise exposure was determined by using a sound level meter. The range of noise was 80-100 dBA. Body Mass Index was also taken for each individual by a standard measure, blood pressure was measured by OMRON sphygmomanometer and serum leptin was measured through venous blood sample analysis enzyme linked immunosorbent assay. Spearman rank order correlation was used to examine the correlations between Blood pressure value (Systolic, Diastolic and Leptin. All the relationships between parameters showed a positive correlation. Systolic and diastolic blood pressure values had a significant correlation to leptin hormone level in comparison to the control. There was a significant relation between leptin and blood pressure. leptin effects on the sympathetic nervous system may provide a partial explanation. Therefore, Leptin might have diverse cardiovascular actions.

  16. Bihormonal model predictive control of blood glucose in people with type 1 diabetes

    DEFF Research Database (Denmark)

    Batora, Vladimir; Tarnik, Marian; Murgas, Jan;

    2014-01-01

    In this paper we present a bihormonal control system that controls blood glucose in people with type 1 diabetes (T1D). We use insulin together with glucagon to mitigate the negative effects of hyper- and hypoglycemia. The system consists of a Kalman filter, a micro-bolus insulin and glucagon...... with an identical insulin MPC, but a glucagon PD controller. The key contribution of the bihormonal MPC is the efficiency of glucagon use. We consider scenarios where the meals are estimated correctly or overestimated and where the insulin sensitivity increases. Both solutions provide tight glucose...... infusion MPC, a mealtime bolus calculator and a CGM providing feedback to the controller. The controller employs a patient data-based prediction model with ARMAX structure. We test the controller using a bihormonal model with time-varying parameters for 3 subjects and compare its performance to a system...

  17. Effect of guar crispbread with cereal products and leguminous seeds on blood glucose concentrations of diabetics.

    Science.gov (United States)

    Jenkins, D J; Wolever, T M; Taylor, R H; Barker, H M; Fielden, H; Jenkins, A L

    1980-11-01

    To compare the effect on blood glucose concentrations of guar incorporated into crispbreads with that of unprocessed high-fibre foods groups of four to six diabetics took a total of seven test breakfasts on separate days. By comparison with a breakfast of wholemeal bread and cheese, guar crispbread combined with bread reduced the area under the glucose response curve to 51% (p soya beans reduced the area to 65% (p soya beans to 25% (p soya beans with lentils to 29% (p < 0.002). Porridge and cornflake breakfasts showed no difference. The favourable results with leguminous seeds may not make such meals more acceptable than meals of guar products, but a combination of leguminous seeds and guar may allow smaller and more acceptable amounts of both to be used. PMID:6253021

  18. Cerebral oxygen and glucose metabolism and blood flow in mitochondrial encephalomyopathy: a PET study

    International Nuclear Information System (INIS)

    Cerebral blood flow (CBF), oxygen metabolism (CMRO2), and glucose metabolism (CMRGlc) were measured using positron emission tomography in five patients diagnosed as having mitochondrial encephalomyopathy. The molar ratio between the oxygen and glucose consumptions was reduced diffusely, as CMRO2 was markedly decreased and CMRGlc was slightly reduced. The CBF showed less changes. The CBF increase on hypercapnia was smaller than normal, though this was not significant. CBF with hypocapnia demonstrated a significant reduction compared with the normal. These results suggest that oxidative metabolism is impaired and anaerobic glycolysis relatively stimulated, due to a primary defect of mitochondrial function, and that mild lactic acidosis occurs in brain tissue because of impaired utilisation of pyruvate in the TCA cycle. As these findings appear to indicate directly a characteristic of this disease, such measurements may be a useful tool for assessment of the pathophysiology and for diagnosis of mitochondrial encephalomyopathy. (orig.). With 1 fig., 4 tabs

  19. Effects of Cinnamomum zeylanicum (Ceylon cinnamon) on blood glucose and lipids in a diabetic and healthy rat model

    OpenAIRE

    Priyanga Ranasinghe; Sanja Perera; Mangala Gunatilake; Eranga Abeywardene; Nuwan Gunapala; Sirimal Premakumara; Kamal Perera; Dilani Lokuhetty; Prasad Katulanda

    2012-01-01

    Objectives: To evaluate short- and long-term effects of Cinnamomum zeylanicum on food consumption, body weight, glycemic control, and lipids in healthy and diabetes-induced rats. Materials and Methods: The study was conducted in two phases (Phase I and Phase II), using Sprague-Dawley rats in four groups. Phase I evaluated acute effects on fasting blood glucose (FBG) (Groups 1 and 2) and on post-oral glucose (Groups 3 and 4) blood glucose. Groups 1 and 3 received distilled-water and Groups 2 a...

  20. Breath acetone concentration decreases with blood glucose concentration in type I diabetes mellitus patients during hypoglycaemic clamps

    OpenAIRE

    Turner, Claire; Walton, Christopher; Hoashi, Shu; Evans, Mark

    2009-01-01

    Conventional wisdom is that breath acetone may be markedly elevated in type 1 diabetes, but that this only occurs during poor blood glucose control and/or intercurrent illness. In contrast, little is known about breath acetone at more representative everyday blood glucose levels in diabetes. We used selected ion flow tube mass spectrometry (SIFT-MS) to monitor the breath of 8 patients with type 1 diabetes mellitus during “insulin clamp” studies in which insulin and glucose were infused into p...

  1. EFFECT OF VITAMIN C ON BLOOD GLUCOSE LEVEL IN RABBITS AND ITS POSSIBLE INTERACTION WITH COMMONLY USED ANTI DIABETIC AGENTS.

    Directory of Open Access Journals (Sweden)

    Chandra Veer Singh

    2015-03-01

    Full Text Available A Effect of Vitamin C on blood glucose and its possible interactions with oral Antidiabetic agents (i.e. Glimepiride, Gliclazide and Metformin in rabbits have been reported in the present study. The study was performed on albino rabbits in which hyperglycemia was induced by giving glucose. We observed that vit.C significantly lowered the blood glucose level but on co-administration with oral antidiabetic agents it antagonized the effect of the latter drugs. It is therefore advisable not to give inadvertent amount of vit.C to diabetic patients just in a belief that antioxidants are helpful to diabetics and do not produce adverse effects.

  2. Association of Serum Ferritin Level with Risk of Incident Abnormal Glucose Metabolism in Southwestern China: a Prospective Cohort Study.

    Science.gov (United States)

    Zhou, Fangli; Zhao, Zhuoxian; Tian, Li; Zheng, Tianpeng; Gao, Yun; Chen, Tao; Yan, Fangfang; Tian, Haoming

    2016-01-01

    This prospective cohort study aimed to analyze the association between serum ferritin levels and the risk of abnormal glucose metabolism (AGM) in Southwestern Chinese population. The 383 subjects who are aged ≥20 years and free of AGM at baseline between in 2007 and in 2008 were included in Southwestern China, and their baseline serum ferritin levels were measured. Among these subjects, 140 subjects were developed into AGM during the follow-up (2008-2012). In logistic regression models, the relative risk in the top versus that in the lowest quartile of serum ferritin levels was 2.86 (p = 0.013) in females and 3.50 (p = 0.029) in males after adjusting the age, gender, family history of diabetes, current smoking, and alcohol; however, serum ferritin levels were not significantly associated with incident of AGM after controlling for metabolic factors (waist circumference, systolic pressure (SBP), triglyceride (TG), and homeostasis model assessment formula insulin resistance (HOMA-IR)). Elevated serum ferritin levels are associated with AGM but not an independent risk factor. PMID:26073512

  3. Investigation of the Blood Glucose Lowering Potential of the Jamaican Momordica charantia (Cerasee) Fruit in Sprague-Dawley Rats.

    Science.gov (United States)

    Burnett, A; McKoy, M L; Singh, P

    2015-09-01

    The Momordica charantia (MC) fruit has been documented to possess antidiabetic properties. However, these studies were not without controversy surrounding the blood glucose-lowering ability and the mechanism of action in diabetes therapy. In an effort to evaluate such claims in the Jamaican MC species known as cerasee, aqueous extracts of the unripe fruit were studied in normal and diabetic rats. Normal male Sprague-Dawley rats were divided into groups (n = 6) orally administered distilled water, 10% dimethyl sulfoxide (DMSO) solution, the aqueous extract (400 mg/kg body weight) and glibenclamide (15 mg/kg body weight), respectively prior to assessment of fasting blood glucose (FBG) concentration. The oral glucose tolerance test (OGTT) was conducted in normoglycaemic rats orally administered distilled water, 10% DMSO solution, glibenclamide (15 mg/kg body weight) or aqueous extracts of the fruit (200 and 400 mg/kg body weight). Blood glucose concentration was also monitored in streptozotocin-induced diabetic rats administered the aqueous extract (250 mg/kg body weight) or water vehicle after an overnight fast. The aqueous extracts showed no hypoglycaemic or antidiabetic activity. However, the administration of the aqueous extracts (200 and 400 mg/kg body weight) resulted in significant improvement in glucose tolerance of glucose-primed normoglycaemic rats during the OGTT. These data suggest that the glucose-lowering mechanism of the Jamaican MC fruit species likely involves altered glucose absorption across the gastrointestinal tract. PMID:26624580

  4. Alternate Immersion in an External Glucose Solution Differentially Affects Blood Sugar Values in Older Versus Younger Zebrafish Adults.

    Science.gov (United States)

    Connaughton, Victoria P; Baker, Cassandra; Fonde, Lauren; Gerardi, Emily; Slack, Carly

    2016-04-01

    Recently, zebrafish have been used to examine hyperglycemia-induced complications (retinopathy and neuropathy), as would occur in individuals with diabetes. Current models to induce hyperglycemia in zebrafish include glucose immersion and streptozotocin injections. Both are effective, although neither is reported to elevate blood sugar values for more than 1 month. In this article, we report differences in hyperglycemia induction and maintenance in young (4-11 months) versus old (1-3 years) zebrafish adults. In particular, older fish immersed in an alternating constant external glucose solution (2%) for 2 months displayed elevated blood glucose levels for the entire experimental duration. In contrast, younger adults displayed only transient hyperglycemia, suggesting the fish were acclimating to the glucose exposure protocol. However, modifying the immersion protocol to include a stepwise increasing glucose concentration (from 1% → 2%→3%) resulted in maintained hyperglycemia in younger zebrafish adults for up to 2 months. Glucose-exposed younger fish collected after 8 weeks of exposure also displayed a significant decrease in wet weight. Taken together, these data suggest different susceptibilities to hyperglycemia in older and younger fish and that stepwise increasing glucose concentrations of 1% are required for maintenance of hyperglycemia in younger adults, with higher concentrations of glucose resulting in greater increases in blood sugar values. PMID:26771444

  5. A novel, disposable, screen-printed amperometric biosensor for glucose in serum fabricated using a water-based carbon ink.

    Science.gov (United States)

    Crouch, Eric; Cowell, David C; Hoskins, Stephen; Pittson, Robin W; Hart, John P

    2005-11-15

    Screen-printed amperometric glucose biosensors have been fabricated using a water-based carbon ink. The enzyme glucose oxidase (GOD) and the electro-catalyst cobalt phthalocyanine were mixed with the carbon ink prior to the screen-printing process; therefore, biosensors are prepared in a one-step fabrication procedure. Optimisation of the biosensor performance was achieved by studying the effects of pH, buffer strength, and applied potential on the analytical response. Calibration studies were performed under optimum conditions, using amperometry in stirred solution, with an operating potential of +500 mV versus SCE. The sensitivity was found to be 1170 nA mM(-1), with a linear range of 0.025-2 mM; the former represents the detection limit. The disposable amperometric biosensor was evaluated by carrying out replicate determinations on a sample of bovine serum. This was achieved by the method of multiple standard additions and included a correction for background currents arising from oxidizable serum components. The mean serum concentration was calculated to be 8.63 mM and compared well with the supplier's value of 8.3 mM; the coefficient of variation was calculated to be 3.3% (n=6). PMID:16242609

  6. [Research of Outlier Samples Elimination Methods for Near-Infrared Spectral Analysis of Blood Glucose].

    Science.gov (United States)

    Lin, Yongzhong; Li, Lina; Lin, Tianliang

    2015-12-01

    For the near-infrared (NIR) spectral analysis of the concentration of blood glucose, the calibration accuracy can be affected because of the existing of outlier samples. In this research, a Monte-Carlo cross validation (MCCV) method is constructed for eliminating outlier samples. The human blood plasma experiment in vitro and the human body experiment in vivo were introduced to evaluate the MCCV method for its application effect in NIR spectral analysis of blood glucose. And the uninformative sample elimination method based on modified uninformative variable elimination (MUVE-USE) was employed in this study for the comparison with MCCV. The results indicated that, like the MUVE-USE method, the outlier samples elimination method based on MCCV could be used to eliminate the outlier samples which came from gross errors (such as bad sample) or system errors (such as baseline drift). In addition, the outlier samples from the random errors of uncertain causes which affect model accuracy can be eliminated simultaneously by MCCV. The elimination of multiple outlier samples is beneficial to the improvement of prediction accuracy of calibration model. PMID:27079108

  7. Cerebral blood flow, glucose use, and CSF ionic regulation in potassium-depleted rats

    International Nuclear Information System (INIS)

    Rats were kept on a low-K+ diet for 25 or 70 days. Local cerebral blood flow (LCBF) and local cerebral glucose utilization (LCGU) were measured in 31 different structures of the brain by means of the [14C]iodoantipyrine and [14C]2-deoxy-D-glucose method. After 25 and 70 days of K+ depletion LCBF was decreased significantly in 27 and 30 structures, respectively, the average decrease being 19 and 25%. In contrast, average LCGU was not changed. Cisternal cerebrospinal fluid (CSF) K+ concentration decreased significantly from 2.65 ± 0.02 mM in controls to 2.55 ± 0.02 mM and 2.47 ± 0.02 mM in the two treated groups. CSF [HCO3-], pH, and Pco2 were increased in K+-depleted animals. These data show that K+ depletion induces an increase in CSF pH and a decrease in CSF K+ concentration, both of which cause a reduction in cerebral blood flow. The increased CSF Pco2 is secondary to the reduction of blood flow, since brain metabolism and arterial Pco2 remained constant

  8. Association between DNA Methylation in Whole Blood and Measures of Glucose Metabolism: KORA F4 Study

    Science.gov (United States)

    Wahl, Simone; Kunze, Sonja; Molnos, Sophie; Volkova, Nadezda; Schramm, Katharina; Carstensen-Kirberg, Maren; Waldenberger, Melanie; Gieger, Christian; Peters, Annette; Illig, Thomas; Prokisch, Holger; Roden, Michael; Grallert, Harald

    2016-01-01

    Epigenetic regulation has been postulated to affect glucose metabolism, insulin sensitivity and the risk of type 2 diabetes. Therefore, we performed an epigenome-wide association study for measures of glucose metabolism in whole blood samples of the population-based Cooperative Health Research in the Region of Augsburg F4 study using the Illumina HumanMethylation 450 BeadChip. We identified a total of 31 CpG sites where methylation level was associated with measures of glucose metabolism after adjustment for age, sex, smoking, and estimated white blood cell proportions and correction for multiple testing using the Benjamini-Hochberg (B-H) method (four for fasting glucose, seven for fasting insulin, 25 for homeostasis model assessment-insulin resistance [HOMA-IR]; B-H-adjusted p-values between 9.2x10-5 and 0.047). In addition, DNA methylation at cg06500161 (annotated to ABCG1) was associated with all the aforementioned phenotypes and 2-hour glucose (B-H-adjusted p-values between 9.2x10-5 and 3.0x10-3). Methylation status of additional three CpG sites showed an association with fasting insulin only after additional adjustment for body mass index (BMI) (B-H-adjusted p-values = 0.047). Overall, effect strengths were reduced by around 30% after additional adjustment for BMI, suggesting that this variable has an influence on the investigated phenotypes. Furthermore, we found significant associations between methylation status of 21 of the aforementioned CpG sites and 2-hour insulin in a subset of samples with seven significant associations persisting after additional adjustment for BMI. In a subset of 533 participants, methylation of the CpG site cg06500161 (ABCG1) was inversely associated with ABCG1 gene expression (B-H-adjusted p-value = 1.5x10-9). Additionally, we observed an enrichment of the top 1,000 CpG sites for diabetes-related canonical pathways using Ingenuity Pathway Analysis. In conclusion, our study indicates that DNA methylation and diabetes-related traits

  9. Identification of transferrin as the principal neptunium-binding protein in the blood serum of rats

    Energy Technology Data Exchange (ETDEWEB)

    Wirth, R.; Taylor, D.M.; Duffield, J.

    1985-01-01

    The binding of 239Np(V) to blood serum components of rats was examined in vivo and in vitro. After gel filtration of the serum using a Sephacryl S-300 column, 98% of the applied activity appeared with protein fractions representing coeluted albumins and transferrin. A separation of the albumin- and transferrin-proteins by ion-exchange chromatography using DEAE-cellulose showed the 239Np being entirely bound to the iron-carrier protein transferrin. The high elution yields from the ion-exchange columns, greater than 90%, suggest that the binding may be quite strong. The binding capacity of transferrin for neptunium in vivo was found to decline when the iron level in blood serum was increased. Precipitation experiments showed that 84 +/- 2% of the 239Np was precipitated with 10% (w/v) trichloracetic acid, 77 +/- 3% with 90% ethanol but only 6 +/- 1% with saturated ammonium sulphate at pH 7.4. The available data indicate that as for plutonium, thorium, americium and curium, the iron transport protein, transferrin, may be the main carrier protein for neptunium in mammalian blood serum.

  10. Thresholds of whole-blood β-hydroxybutyrate and glucose concentrations measured with an electronic hand-held device to identify ovine hyperketonemia.

    Science.gov (United States)

    Pichler, M; Damberger, A; Schwendenwein, I; Gasteiner, J; Drillich, M; Iwersen, M

    2014-03-01

    Metabolic disorders, especially hyperketonemia, are very common in dairy sheep. The whole-blood concentrations of β-hydroxybutyrate (BHBA) and glucose can be determined by commercially available electronic hand-held devices, which are used in human medicine and for the detection of ketosis in dairy cows. The aim of this study was to evaluate the suitability of the hand-held device Precision Xceed (PX; Abbott Diabetes Care Inc., Abbott Park, IL) to detect hyperketonemia in ewes. An additional objective of this study was to evaluate the agreement between samples obtained by minimal invasive venipuncture of an ear vein and measurements of whole-blood samples from the jugular vein (vena jugularis, v. jug.). Blood samples taken from the v. jug. were collected from 358 ewes on 4 different farms. These samples and a blood drop obtained from an ear vein were analyzed simultaneously on farm with the PX. For method comparison, the samples obtained from the v. jug. were also analyzed by standard methods, which served as the gold standard at the Central Laboratory of the University of Veterinary Medicine Vienna, Austria. The correlation coefficients between the serum BHBA concentration and the concentrations measured with the hand-held meter in the whole blood from an ear vein and the v. jug. were 0.94 and 0.96, respectively. The correlation coefficients of plasma and whole-blood glucose concentration were 0.68 for the v. jug. and 0.47 for the ear vein. The mean glucose concentration was significantly lower in animals classified as hyperketonemic (BHBA ≥ 1.6 mmol/L) compared with healthy ewes. Whole-blood concentrations of BHBA and glucose measured with the PX from v. jug. showed a constant negative bias of 0.15 mmol/L and 8.4 mg/dL, respectively. Hence, a receiver operating characteristic analysis was performed to determine thresholds for the PX to detect hyperketonemia in ewes. This resulted in thresholds for moderate ketosis of BHBA concentrations of 0.7 mmol/L in blood

  11. Patient and healthcare professional satisfaction with a new, high accuracy blood glucose meter with color range indicator and wireless connectivity.

    Science.gov (United States)

    Katz, Laurence B; Grady, Mike; Stewart, Lorna; Cameron, Hilary

    2016-07-01

    Accurate self-monitoring of blood glucose is a key component of effective self-management of glycemic control. The OneTouch VerioFlex(™) (OTVF) blood glucose monitoring system (BGMS) was evaluated for accuracy in a clinical setting. Patients also used OTVF for a 1-wk trial period and reported their level of satisfaction with meter features. In a separate study, healthcare professionals used an on-line simulator of the BGMS and answered questions about its potential utility to their patients. OTVF was accurate over a wide glucose range and met lay user and system accuracy blood glucose standards described in ISO15197:2013 as well as the accuracy requirements to fulfill US FDA expectations for 510(k) clearance of BGMS. Patients and healthcare professionals felt the features of OTVF, which has the capability to connect wirelessly to mobile devices and interact wirelessly with diabetes management software, could provide significant benefits to them or their patients. PMID:27232211

  12. Protective effect of Cassia glauca Linn. on the serum glucose and hepatic enzymes level in streptozotocin induced NIDDM in rats

    OpenAIRE

    Farswan, Mamta; MAZUMDER, PAPIYA MITRA; Percha, V.

    2009-01-01

    Objective: The objective of the present study was to investigate the hypoglycemic and hepatoprotective effect of Cassia glauca leaf extracts on normal and non insulin dependent diabetes mellitus (NIDDM) in rats. The study was further carried out to investigate the effect of different fractions of the active extract of Cassia glauca, on normal and NIDDM rats, and the effect of active fraction on the blood glucose and hepatic enzymes level. Methods: Diabetes was induced by streptozotocin (STZ) ...

  13. Studies on glucose metabolism and blood perfusion in childhood partial seizure by positron emission CT

    International Nuclear Information System (INIS)

    To investigate the glucose metabolism and blood perfusion of the interictal epileptic focus, 15 positron emission tomography (PET) measurements were performed in 14 children with partial seizures (2 with simple partial seizures, 2 with complex partial seizures, and 10 with partial seizures evolving to secondary generalized seizures), comprising 7 males and 7 females aged 1 to 12 years old at the onset of the epileptic seizures. The intervals between the seizure onset and PET examinations were 1 month to 7 years (mean 3 1/4 years). Radiopharmaceuticals such as 11C-glucose, 11CO2 and 11CO were used as indicators of local cerebral glucose metabolism, blood perfusion and blood flow, respectively. Apart from 2 cases, none of the patients showed abnormal x-ray computed tomographic scans (X-CT). The abnormal X-CT findings included cortical atrophy of the cerebrum apart from the epiletic focus in one case and cavum vergae in the other. Hypometabolism and hypoperfusion at the epileptic focus were observed in 10 patients undergoing single examinations who had suffered from epileptic seizures for more than 1 year. Out of 4 patients who had suffered from epileptic seizures for 1 year or less, one revealed a zone of hypometabolism and hypoperfusion in the epileptic focus and expanded region larger than that of the epileptic focus on the electroencephalogram. Two other patients revealed a zone of hypometabolism and hypoperfusion in an area contralateral to the epileptic focus. In the remaining one patient, PET examinations were performed twice. The initial PET pictures one year after seizure onset revealed a zone of hypermetabolism and hyperperfusion in the cerebellum ipsilateral to the epileptic focus, and the second PET at 6 months after the initial examination revealed hypometabolism and hypoperfusion in the focus, similarly to the 10 cases mentioned above. (J.P.N.)

  14. National blood requirement, serum ALT and hepatitis in Ethiopian blood donors.

    Science.gov (United States)

    Zawde, D; Sisay, Y

    1991-10-01

    To appraise the national blood requirement and supply, and to determine the impact of alanine aminotransferase (ALT) and hepatitis B surface antigen (HBsAg) screening on the blood supply, 407 random blood donor sera were tested for HBsAg, human immunodeficiency virus (HIV), and ALT activity. HBsAg and anti-HIV antibody were determined by the enzyme linked immunosorbent assay (ELISA) technique using Hepanostica and Welcozyme kits, respectively. The Western Blot test was performed to confirm anti-HIV positive sera by the ELISA technique. ALT was determined by an automated photometer using ALAT kits and serologic testing for syphilis was done by the rapid plasma reagin (RPR) test. The amount of blood required in Ethiopia and the actual supply was calculated on the basis of the number and type of hospital beds in Addis Abeba and the amount of blood transfusions in units per hospital bed. The results showed that the combined donor and unit rejection rate was 34.6%. The annual blood requirement was 7 units for emergency and 4 units for nonemergency beds. The national blood requirement in 1989 was 64,350-80,000 units, but the supply met only a third of the requirement. The mean and 2SD cut off ALT levels were 28 and 69 IU/L, respectively. ALT was elevated in 9.1% of HBsAg positive but apparently healthy donors, while HBsAg screening eliminated 25% of those with elevated ALT activity. This data suggests that there is a serious blood shortage in Ethiopia and that the currently supplied blood is relatively unsafe in terms of hepatitis.(ABSTRACT TRUNCATED AT 250 WORDS) PMID:1659534

  15. 热性惊厥与铁锌元素、血糖、白细胞的关系%Study on the Relationship between Febrile Convulsion and Iron Zinc, WBC, Blood Glucose

    Institute of Scientific and Technical Information of China (English)

    张秀卿; 宋滨东; 徐桂霞; 王丽君; 马延博

    2015-01-01

    目的观察热性惊厥患儿血清铁、锌元素、血糖、白细胞的水平,并研究探讨两者间的临床相关性。方法对2012年6月~2013年5月因热性惊厥而前来就诊的80例患儿,设为观察组。同期随机选取儿保门诊健康体检儿童80例设为对照组,两组入选儿童均抽血做血清铁、锌、血糖、血常规测定。结果热性惊厥患儿血铁、锌、血糖、白细胞较对照组有显著性差异(<0.05)。结论热性惊厥患儿存在铁锌元素减少,白细胞及血糖升高。%Objective Observe the level of serum iron, zinc, white blood cel s, blood glucose in febrile convulsion children, and to study the clinical cor elation. Methods 80 children because of febrile convulsion to the hospital were selected as the observation group during the period from 2012 June to 2013 May. At the same period 80 healthy children from child health clinic were randomly selected as control group, two groups children were examined serum iron, zinc, blood glucose, blood routine. Results The children with febrile convulsion the white blood cel s and iron, zinc, blood glucose had significant dif erence compared with the control ( <0.05). Conclusion The level of iron and zinc in children with febrile convulsion reduces, white blood cel s and blood glucose increase.

  16. Evaluation of Glucose-6-Phosphate Dehydrogenase stability in stored blood samples

    OpenAIRE

    Jalil, Norunaluwar; Azma, Raja Zahratul; Mohamed, Emida; Ithnin, Azlin; Alauddin, Hafiza; Baya, Siti Noor; Othman, Ainoon

    2016-01-01

    Glucose-6-Phosphate Dehydrogenase (G6PD) deficiency is the commonest cause of neonatal jaundice in Malaysia. Recently, OSMMR2000-D G6PD Assay Kit has been introduced to quantitate the level of G6PD activity in newborns delivered in Universiti Kebangsaan Malaysia Medical Centre (UKMMC). As duration of sample storage prior to analysis is one of the matters of concern, this study was conducted to identify the stability of G6PD enzyme during storage. A total of 188 cord blood samples from normal ...

  17. A Soluble Activin Receptor Type IIB Does Not Improve Blood Glucose in Streptozotocin-Treated Mice

    OpenAIRE

    Wang, Qian; Guo, Tingqing; Portas, Jennifer; McPherron, Alexandra C.

    2015-01-01

    Type 1 diabetes mellitus (T1DM), or insulin dependent DM, is accompanied by decreased muscle mass. The growth factor myostatin (MSTN) is a negative regulator of muscle growth, and a loss of MSTN signaling has been shown to increase muscle mass and prevent the development of obesity, insulin resistance and lipodystrophic diabetes in mice. The effects of MSTN inhibition in a T1DM model on muscle mass and blood glucose are unknown. We asked whether MSTN inhibition would increase muscle mass and ...

  18. Integrated Self-Monitoring of Blood Glucose System: Handling Step Analysis

    OpenAIRE

    Freckmann, Guido; Schmid, Christina; Ruhland, Katharina; Baumstark, Annette; Haug, Cornelia

    2012-01-01

    Self-monitoring of blood glucose (SMBG) implicates a number of handling steps with the meter and the lancing device. Numerous user errors can occur during SMBG, and each step adds to the complexity of use. This report compares the required steps to perform SMBG of one fully integrated (the second generation of the Accu-Chek® Mobile), three partly integrated (Accu-Chek Compact Plus, Ascensia® Breeze®2, and Accu-Chek Aviva), and six conventional (Bayer Contour®, Bayer Contour USB, BGStar™, Free...

  19. Frequency and motives of blood glucose self-monitoring in type 1 diabetes

    DEFF Research Database (Denmark)

    Hansen, M.V.; Pedersen-Bjergaard, U.; Heller, S.R.;

    2009-01-01

    AIMS: Recommendations for self-monitoring of blood glucose (SMBG) from the DCCT have not been implemented with the same rigour as recommendations for intensifying insulin therapy. We assessed the frequency of and motives for SMBG and compared SMBG behaviour with clinical, behavioural and...... hypoglycaemia and awareness of hypoglycaemia were independently associated with testing behaviour, whereas the presence of late diabetic complications was not. Lower HbA1c was associated with more frequent testing. CONCLUSION: Patient compliance regarding SMBG is limited. Thus, almost two thirds of the patients...

  20. Direct determination of sodium and potassium in blood serum by flow injection and atomic absorption spectrophotometry

    International Nuclear Information System (INIS)

    A simple and reliable method for the measurement of sodium and potassium in blood serum without any sample dilution by using flow injection and atomic absorption spectrophotometry is described. A sample throughout of 100 measurements per hour is possible. The coefficient of variation for within-run determination was about 1,14 and 2,36% for sodium and potassium, respectively, in serum samples (n=10). The method is easily adaptable to pediatric research, because of the low required sample volume of 5ul. (Author)

  1. Trace element analysis of human blood serum by neutron activation analysis

    International Nuclear Information System (INIS)

    An attempt was made to determine if there is any correlation between trace element concentrations in human blood serum and some specific diseases. The serum samples of the patients suffering from cancer, Down syndrome, and Banti syndrome were analyzed by the neutron activation method and compared with the trace element concentrations observed among clinically healthy men. The cancer patients had concentrations in Rb, Mn, Fe, Co, Cu, Zn, Al and Se below normal. The Down syndrome patients were found to have similar deficiencies in Cr, Mn, Fe, Co, Zn, Cu and Sb. (author)

  2. The impact of high-dose vitamin C on blood glucose testing in ¹⁸F-FDG PET imaging.

    Science.gov (United States)

    Bahr, Rebekah L; Wilson, Don C

    2015-03-01

    Complementary and alternative therapies in addition to standard oncology protocols are commonly sought by cancer patients; however, few patients disclose their complementary treatments to their cancer care team. A lack of communication may result in unforeseen side effects and the potential for some alternative therapies to interfere with or inhibit conventional treatment. High-dose vitamin C therapy, in particular, may lead to an inability to measure a patient's blood glucose level before (18)F-FDG injection for PET/CT scanning. We report a case of a 52-y-old woman referred for (18)F-FDG PET/CT to evaluate the extent of recurrent colorectal cancer. The PET/CT scan immediately followed a single intravenous dose of 25 g of ascorbic acid from her naturopath. A glucometer that applies the glucose oxidase method for measuring fasting blood glucose was used, for which high doses of vitamin C are listed as a contraindication. The high concentration of ascorbic acid in the patient's blood sample interfered with the chemical reaction on the glucose strip, and therefore no blood glucose measurement could be attained. With more patients receiving alternative and complementary cancer therapies, it is important to know what the implications of orthomolecular therapy might be on routine blood glucose testing for (18)F-FDG PET scans. (18)F-FDG is in direct competition with glucose; therefore, elevated blood glucose levels will cause a decrease in (18)F-FDG absorption and may lead to a false-negative scan. PMID:25104819

  3. Changes of Leptin Level in Serum, Cord Venous Blood and Placenta in Women with Gestational Diabetes Mellitus and Correlations with Insulin and C-Peptide

    Institute of Scientific and Technical Information of China (English)

    Jin-ming ZHU; Li SHI; Xiao-yuan LU; Rong-rong ZHANG; Min LI; Xiao-ning ZHANG

    2014-01-01

    ObjectiveTo investigate the changes of leptin levels in serum, cord venous blood and placenta and examine the possible relationships among them in women with gestational diabetes mellitus (GDM).Methods This case-control study was performed in 40 women with GDM and 40 normal women. The women with GDM received dietary advice, blood glucose monitoring and insulin treatment when necessary. Maternal serum and venous cord blood leptin, insulin and C-peptide levels were detected by ELISA. The expression level of leptin in placenta was measured by an immunohistochemical method. Results1. Leptin, insulin and C-peptide levels in serum of the women with GDM were significantly higher than those of the normal women (P<0.01,P<0.05, P<0.01). 2. Cord venous blood leptin and insulin in GDM group were significantly higher than in the normal group (P<0.01,P<0.05). No significant difference was found in cord venous blood C-peptide between two groups. 3. The expression of placenta leptin protein was significantly higher in GDM women than in normal women (P<0.01). There were positive correlations between placental leptin, cord venous leptin and insulin, birth weight, ponderal index in GDM women (r=0.37,P<0.05;r=0.39,P<0.05;r=0.53,P<0.01;r=0.54,P<0.01). However, there was no correlation between placental leptin and serum leptin.Conclusion The women with GDM and their fetuses suffer from hyperleptinaemia and hyperinsulinaemia. In the women with GDM, there was disorder in the interaction between leptin and insulin. The GDM women and their fetuses may be prone to insulin resistance and leptin resistance in late pregnancy.

  4. Blood glucose meter with biochemical analyzer blood glucose than on analysis%血糖仪与生化分析仪测定血糖的比对分析

    Institute of Scientific and Technical Information of China (English)

    秦亚军; 张保龙; 杨笑琼

    2013-01-01

      目的:探讨血糖仪与生化分析仪测定血糖的比对情况,为合理选择血糖检测方法提供参考。方法:选择2009年2月~2012年8月在我院门诊住院患者糖尿病人员共120例,上肢肘静脉和手指末端分别采血进行生化分析仪与血糖仪检测。结果:经过检测,生化分析仪检测的血糖值为(6.30±0.22)mmol/L,血糖仪检测的血糖值为(6.25±1.89)mmol/L,两种方法检测值对比无明显差异(P>0.05)。但是血糖仪检测的重复性不强,导致s值比较大。结论:血糖仪在糖尿病患者的血糖监测中有很大的局限性,在临床中要将血糖仪的监测和医院血糖检测结合使用,才能达到良好的血糖控制目的。%Objective:To investigate the blood glucose meter and biochemical analyzer than on blood sugar, and provide a reference for the reasonable choice of blood glucose testing method. Methods:From February 2009 to August 2012, a total of 120 cases of staff in our hospital hospitalized patients with diabetes, venous and finger blood samples were collected at the end of the upper limb elbow biochemical analyzer and blood glucose meter testing. Results:After testing, the the biochemical analyzer blood sugar value of (6.30± 0.22)mmol/L, blood glucose meter testing blood sugar value of (6.25±1.89)mmol/L, two methods for detecting the value of contrast was no significant difference(P>0.05). However, the reproducibility of the blood glucose meter detecting is not strong, resulting in the value of s is relatively large. Conclusion: The blood glucose meter glucose monitoring in diabetes has many limitations, combined To glucometer monitoring and hospital blood glucose testing in clinical use, in order to achieve good glycemic control purposes.

  5. Effect of Ethanolic Extract of Fragaria Vesca on serum glucose levels and body weight in diet induced obese rats

    OpenAIRE

    Venkat ramana Yella; Asha P Dass

    2015-01-01

    Objective: to evaluate the effect of ethanolic extract Fragaria Vesca on serum glucose levels in diet induced obese rats.Material and methods: Male Wister albino rats weighing 200- 250 gm, were divided into 3 groups of 6 animals each. The animals of all the groups except normal group were given a lipid diet consisting of cholesterol (1%), cholic acid (0.5%), casein (20%), choline (0.25%), d-l-methionin1(0.4%), coconut oil (25%), multi vitamin mix (3.5%) and sucrose (48.4%) with standard pelle...

  6. Design of a prospective clinical study on the agreement between the Continuous GlucoseMonitor, a novel device for CONTinuous ASSessment of blood GLUcose levels, and the RAPIDLab® 1265 blood gas analyser: The CONTASSGLU study

    Directory of Open Access Journals (Sweden)

    Zimmermann Johannes B

    2012-09-01

    Full Text Available Abstract Background Although a device is needed to continuously measure blood glucose levels within an intensive care setting, and several large-scale prospective studies have shown that patients might benefit from intensive insulin, potassium, or glucose therapy during intensive care, no devices are currently available to continuously assess blood glucose levels in critically ill patients. We conceived the study described here to evaluate the clinical use of the Continuous Glucose Monitor (CGM performed via a central vein, and to determine the impact of phenomena, such as drift and shift, on the agreement between the CGM and a RAPIDLab® 1265 blood gas analyser (BGA. Methods/design In the CONTinuous ASSessment of blood GLUcose (CONTASSGLU study, up to 130 patients under intensive care will be fitted with the CGM, an ex vivo device that continuously measures blood glucose and lactate levels. Readings from the device taken 8 h after initial placement and calibration will be compared with values measured by a BGA. For this study, we chose the BGA as it is an established standard point-of-care device, instead of the devices used in certified central laboratories. Nevertheless, we will also independently compare the results from the point-of-care BGA with those determined by a central laboratory-based device. Blood samples will be collected from each patient from the same site in which the CGM will measure blood glucose. Consequently, each participant will serve as their own control, and no randomisation is necessary. The 95% limits of agreement and the corresponding confidence intervals will be calculated and compared with a prespecified clinically acceptable relative difference of 20%. Discussion Several attempts have been made to develop a device to continuously measure blood glucose levels within an intensive care setting or to use the devices that were originally designed for diabetes management, as several of these devices are already

  7. Alanine Aminotransferase Is Associated with an Adverse Nocturnal Blood Glucose Profile in Individuals with Normal Glucose Regulation

    OpenAIRE

    Zhou, Jian; Mo, Yifei; Li, Hong; Ran, Xingwu; Yang, Wenying; LI Qiang; Peng, Yongde; Li, Yanbing; Gao, Xin; Luan, Xiaojun; Wang, Weiqing; Jia, Weiping

    2013-01-01

    Objective Although the association between alanine aminotransferase (ALT) levels and risk of type 2 diabetes is well-studied, the effects of slightly increased ALT levels within the normal range on the temporal normal glucose profile remains poorly understood. Methods A total of 322 Chinese subjects without impaired glucose tolerance or previous diagnoses of diabetes were recruited for study from 10 hospitals in urban areas across China. All subjects wore a continuous glucose monitoring (CGM)...

  8. The Effect of Chlorella vulgaris Supplementation on Liver Enzymes, Serum Glucose and Lipid Profile in Patients with Non-Alcoholic Fatty Liver Disease

    Directory of Open Access Journals (Sweden)

    Mehrangiz Ebrahimi-Mameghani

    2014-07-01

    Full Text Available Background: Non-alcoholic fatty liver disease (NAFLD is becoming a public health problem worldwide and using microalgae is a new approach on its treatment. The aim of this study was to investigate the effect of Chlorella vulgaris supplementation on liver enzymes, serum glucose and lipid profile in patients with NAFLD. Methods: This double-blind randomized placebo-controlled clinical trial was conducted on 60 NAFLD patients from specialized clinics of Tabriz University of Medical Sciences from December 2011 to July 2012. The subjects were randomly allocated into 2 groups: 1 “intervention” (n=30 received 400 mg/day vitamin E plus four 300 mg tablets of Chlorella vulgaris and, 2 “placebo” (n=30 received 400 mg/day vitamin E and four placebo tablets per day for 8 weeks. Weight, liver enzymes and metabolic factors were assessed in fasting serum and dietary data was collected at baseline and end of the study. Results: Weight, liver enzymes, fasting blood sugar (FBS and lipid profile decreased significantly in both groups (P<0.05. The differences in weight, ALP and FBS between the two groups were statistically significant (P=0.01, P=0.04 and P=0.02, respectively. Conclusion: C. vulgaris seems to improve FBS and lipid profile and therefore could be considered as an effective complementary treatment in NAFLD.

  9. Detection of Babesia bovis in blood samples and its effect on the hematological and serum biochemical profile in large ruminants from Southern Punjab

    Institute of Scientific and Technical Information of China (English)

    Samreen Zulfiqar; Ali Saeed; Furhan Iqbal; Sadia Shahnawaz; Muhammad Ali; Arif Mahmood Bhutta; Shahid Iqbal; Sikandar Hayat; Shazia Qadir; Muhammad Latif; Nazia Kiran

    2012-01-01

    Objective:To determine the presence of Babesia bovis (B. bovis) in large ruminants in southern Punjab and its effect on hematological and serum biochemical profile of host animals. Methods:Blood samples were collected from 144 large ruminants, including 105 cattle and 39 buffaloes, from six districts in southern Punjab including Multan, Layyah, Muzaffar Garh, Bhakar, Bahawalnagar and Vehari. Data on the characteristics of animals and herds were collected through questionnaires. Different blood (hemoglobin, glucose) and serum (ALT, AST, LDH, cholesterol) parameters of calves and cattle were measured and compared between parasite positive and negative samples to demonstrate the effect of B. bovis on the blood and serological profile of infected animals. Results:27 out of 144 animals, from 5 out of 6 sampling districts, produced the 541-bp fragment specific for B. bovis. Age of animals (P=0.02), presence of ticks on animals (P=0.04) and presence of ticks on dogs associated with herds (P=0.5) were among the major risk factors involved in the spread of bovine babesiosis in the study area. ALT concentrations were the only serum biochemical values that significantly varied between parasite positive and negative cattle. Conclusions:This study has reported for the first time the presence of B. bovis in large ruminant and the results can lead to the prevention of babesiosis in the region to increase the livestock output.

  10. Serum cortisol level and its correlation to serum insulin and fasting blood sugar levels in patients with type 2 diabetes mellitus

    International Nuclear Information System (INIS)

    Objective: To investigate the change of serum cortisol levels and its correlation to blood sugar and serum insulin levels in patients with type 2 diabetes mellitus. Methods: Blood sugar with oxidase method and serum cortisol insulin levels with RIA (8 AM fasting specimen) were measured in 26 patients with type 2 diabetes mellitus and 30 controls. Results: The serum cortisol levels in the diabetic patients were significantly higher than those in the controls (P<0.01). The cortisol levels were positively correlated to the blood sugar levels (r=0.32, p<0.01), but not correlated to insulin levels. Conclusion: There were cortisol secretion disturbances in patients with type 2 diabetes

  11. The Efficacy of Boswellia Serrata Gum Resin for Control of Lipid Profile and Blood Glucose in Diabetic Patients

    Science.gov (United States)

    Mehrzadi, Saeed; Tavakolifar, Bahreh; Huseini, Hasan Fallah; Mosavat, Seyed Hamdollah; Heydari, Mojtaba

    2016-01-01

    Background: Regarding preclinical evidence for antidiabetic effects of Boswellia serrata, we evaluated anti-hyperglycemic and lipid-lowering effects of Boswellia serrate gum resin in type 2 diabetic patients in a double-blind randomized placebo-control trial. Methods: Fifty-six diabetic patients were randomly allocated to two groups to receive 250 mg of the Boswellia serrate gum resin or placebo twice daily for 8 weeks, in addition to their routine antidiabetic treatments. Fasting blood sugar (FBS), glycosylated hemoglobin (HbA1c), insulin level, total cholesterol, low-density lipoprotein (LDL), high-density lipoprotein (HDL) and triglyceride of serum were measured before and after the intervention. Results: Although there was a considerable reduction after the intervention in FBS (P=0.04), HbA1c (P=0.02) and triglyceride (P=0.01) in the Boswellia serrate gum resin group, no significant difference was observed in all outcome measures between the two groups at the end of the study (FBS P=0.09, HbA1c P=0.20, total cholesterol P=0.31, LDL P=0.49, HDL P=0.10, triglyceride P=0.78 and insulin level P=0.86). Conclusion: The current study showed the 8 weeks complementary use of Boswellia serrate gum resin with a daily dose of 500 mg had no better glucose and lipid lowering effect than placebo in diabetic patients.

  12. Evaluation of Glucose-6-Phosphate Dehydrogenase stability in stored blood samples.

    Science.gov (United States)

    Jalil, Norunaluwar; Azma, Raja Zahratul; Mohamed, Emida; Ithnin, Azlin; Alauddin, Hafiza; Baya, Siti Noor; Othman, Ainoon

    2016-01-01

    Glucose-6-Phosphate Dehydrogenase (G6PD) deficiency is the commonest cause of neonatal jaundice in Malaysia. Recently, OSMMR2000-D G6PD Assay Kit has been introduced to quantitate the level of G6PD activity in newborns delivered in Universiti Kebangsaan Malaysia Medical Centre (UKMMC). As duration of sample storage prior to analysis is one of the matters of concern, this study was conducted to identify the stability of G6PD enzyme during storage. A total of 188 cord blood samples from normal term newborns delivered at UKMMC were selected for this study. The cord bloods samples were collected in ethylene-diamine-tetra-acetic acid (EDTA) tubes and refrigerated at 2-8 °C. In addition, 32 out of 188 cord blood samples were spotted on chromatography paper, air-dried and stored at room temperature. G6PD enzyme activities were measured daily for 7 days using the OSMMR2000-D G6PD Assay Kit on both the EDTA blood and dried blood samples. The mean value for G6PD activity was compared between days of analysis using Student Paired T-Test. In this study, 172 out of 188 cord blood samples showed normal enzyme levels while 16 had levels corresponding to severe enzyme deficiency. The daily mean G6PD activity for EDTA blood samples of newborns with normal G6PD activity showed a significant drop on the fourth day of storage (p deficient G6PD activity, significant drop was seen on third day of storage (p = 0.002). Analysis of dried cord blood showed a significant reduction in enzyme activity as early as the second day of storage (p = 0.001). It was also noted that mean G6PD activity for spotted blood samples were lower compared to those in EDTA tubes for all days (p = 0.001). Thus, EDTA blood samples stored at 2-8 °C appeared to have better stability in terms of their G6PD enzyme level as compared to dried blood samples on filter paper, giving a storage time of up to 3 days. PMID:27103895

  13. Diabetes Technology: Markers, Monitoring, Assessment, and Control of Blood Glucose Fluctuations in Diabetes

    Directory of Open Access Journals (Sweden)

    Boris P. Kovatchev

    2012-01-01

    Full Text Available People with diabetes face a life-long optimization problem: to maintain strict glycemic control without increasing their risk for hypoglycemia. Since the discovery of insulin in 1921, the external regulation of diabetes by engineering means has became a hallmark of this optimization. Diabetes technology has progressed remarkably over the past 50 years—a progress that includes the development of markers for diabetes control, sophisticated monitoring techniques, mathematical models, assessment procedures, and control algorithms. Continuous glucose monitoring (CGM was introduced in 1999 and has evolved from means for retroactive review of blood glucose profiles to versatile reliable devices, which monitor the course of glucose fluctuations in real time and provide interactive feedback to the patient. Technology integrating CGM with insulin pumps is now available, opening the field for automated closed-loop control, known as the artificial pancreas. Following a number of in-clinic trials, the quest for a wearable ambulatory artificial pancreas is under way, with a first prototype tested in outpatient setting during the past year. This paper discusses key milestones of diabetes technology development, focusing on the progress in the past 10 years and on the artificial pancreas—still not a cure, but arguably the most promising treatment of diabetes to date.

  14. 快速血糖仪与全自动生化分析仪检测结果一致性比较%Comparison of the detection results consistency of fast blood glucose meter and automatic biochemical analyzer

    Institute of Scientific and Technical Information of China (English)

    杨晓红

    2015-01-01

    Objective:To compare the detection results consistency of fast blood glucose meter and automatic biochemical analyzer.Methods:40 cases of healthy controls and diabetic patients were selected.They were all given fast blood glucose meter and automatic biochemical analyzer to detect the glucose value.Results:The venous serum glucose values of automatic biochemical analyzer were all higher than the venous whole blood glucose values of fast blood glucose meter,and the difference was no statistically significant(P>0.05).Conclusion:The venous whole blood glucose values of fast blood glucose meter are compared with serum glucose values of automatic biochemical analyzer.Because the specimen sources are consistent,the operation process is simple.So it is suggested that medical institutions can adopt this method for regular comparison experiment.%目的:对快速血糖仪与全自动生化分析仪检测结果的一致性进行比较。方法:选取健康体检和糖尿病患者40例,均采用快速血糖仪和全自动生化分析仪进行血糖值的检测。结果:全自动生化分析仪所测静脉血清血糖值均高于快速血糖仪所测静脉全血血糖值,差异无统计学意义(P>0.05)。结论:快速血糖仪的静脉全血血糖值与全自动生化分析仪的血清血糖值比较,因标本来源一致,操作流程简单,故建议医疗机构可采用此方法进行定期比对实验。

  15. Effect of Syzygium Aromaticum (CLOVE) Extract on Blood Glucose Level in Streptozotocin induced Diabetic Rats

    International Nuclear Information System (INIS)

    Objective: To evaluate the glucose lowering effect of 50% ethanol extract of Syzygium aromaticum in comparison with that of standard insulin in streptozotocin induced diabetic rats. Study Design: Randomized control trial. Place and Duration of Study: National Institute of Health Islamabad. Jul 2011- Dec 2011 Material and Methods: It was carried out on 48 adult rats of Sprague dawley specie. Rats were equally divided into 6 groups (I-VI). Group - I served as control. Diabetes was induced by giving single intraperitoneal injection of STZ in Group II to VI. Group-II served as diabetic control, while groups III, IV, V and VI served as experimental groups. Group III, IV and V rats received 50% ethanol extract of Syzygium aromaticum at a dose of 250, 500 and 750 mg/kg body weight respectively for sixty days. Group VI (standard) received humulin insulin 70/30 at dose of 0.6 units<-kg body weight subcutaneously bid for sixty days. Fasting blood samples were taken at zero day, 15 day, 30 day and 60 day after giving injection STZ. Although Syzygium aromaticum with the doses of 250, 500 and 750 mg/kg body weight and insulin reduced the level of glucose in rats but on comparison Syzygium aromaticum 750 mg=kg dose reduced glucose more effectively than 250 and 500 mg/kg dose. While in group III, IV subjects, blood glucose levels remained above normal level. In group VI receiving insulin the level of this parameter remained almost closer to group IV rats. On studying the weight of the animals after receiving STZ there was initial reduction in the weight of all the experimental groups but after receiving the extract of plant improvement was seen and the weight of group V getting 750 mg=kg/body weight of Syzygium aromaticum became almost closer to the weight of control group. Conclusion: Syzygium aromaticum extract has glucose lowering effect in STZ induced diabetic rats and this effect is dose related and the dose of 750 mg/kg body weight has produced maximum effect. (author)

  16. Increased serum erythropoietin concentration after allogeneic compared with autologous blood transfusion.

    Science.gov (United States)

    Avall, A; Hyllner, M; Swolin, B; Bengtson, J p; Carlsson, L; Bengtsson, A

    2002-12-01

    Serum erythropoietin (sEPO) level is known to increase as hemoglobin (Hb) concentration decreases during and after preoperative autologous blood donation (PAD). The endogenous erythropoietin (EPO) production after allogeneic blood transfusion has not to our knowledge, been studied. The aim of the present study was to determine whether there is, after surgery, any change in sEPO concentration after allogeneic blood transfusion, and whether there is any difference in EPO response after autologous or allogeneic blood transfusion. Thirty-one patients approaching total hip-joint replacement surgery, were randomized to receive either allogeneic red blood cells (n = 15) or predeposited autologous whole blood transfusion (n = 16). The relationship between Hb, sEPO, and reticulocytes in the recipients were repeatedly analyzed before, during and after surgery. The Hb followed an expected pattern, with a decreased concentration after PAD in the autologous group, then in both groups after surgery. The sEPO concentration was significantly higher in the allogeneic than in the autologous group on day one and day 4-5 postoperatively. The reticulocyte level, on the contrary, was higher in the autologous patients before, one hour after, and one day after surgery. The study showed a greater increase in sEPO concentration after allogeneic blood transfusion than after autologous blood transfusion. There may be an inverse relationship between sEPO and the reticulocyte level. PMID:12509214

  17. Blood serum and BSA, but neither red blood cells nor hemoglobin can support vitellogenesis and egg production in the dengue vector Aedes aegypti

    Directory of Open Access Journals (Sweden)

    Kristina K. Gonzales

    2015-05-01

    Full Text Available Aedes aegypti is the major vector of dengue, yellow fever and chikungunya viruses that put millions of people in endemic countries at risk. Mass rearing of this mosquito is crucial for strategies that use modified insects to reduce vector populations and transmission of pathogens, such as sterile insect technique or population replacement. A major problem for vector mosquito mass rearing is the requirement of vertebrate blood for egg production since it poses significant costs as well as potential health hazards. Also, regulations for human and animal use as blood source can pose a significant obstacle. A completely artificial diet that supports egg production in vector mosquitoes can solve this problem. In this study, we compared different blood fractions, serum and red blood cells, as dietary protein sources for mosquito egg production. We also tested artificial diets made from commercially available blood proteins (bovine serum albumin (BSA and hemoglobin. We found that Ae. aegypti performed vitellogenesis and produced eggs when given whole bovine blood, serum, or an artificial diet containing BSA. Conversely, egg production was impaired after feeding of the red blood cell fraction or an artificial diet containing only hemoglobin. We also found that egg viability of serum-fed mosquitoes were comparable to that of whole blood and an iron supplemented BSA meal produced more viable eggs than a meal containing BSA alone. Our results indicate that serum proteins, not hemoglobin, may replace vertebrate blood in artificial diets for mass mosquito rearing.

  18. Blood serum and BSA, but neither red blood cells nor hemoglobin can support vitellogenesis and egg production in the dengue vector Aedes aegypti.

    Science.gov (United States)

    Gonzales, Kristina K; Tsujimoto, Hitoshi; Hansen, Immo A

    2015-01-01

    Aedes aegypti is the major vector of dengue, yellow fever and chikungunya viruses that put millions of people in endemic countries at risk. Mass rearing of this mosquito is crucial for strategies that use modified insects to reduce vector populations and transmission of pathogens, such as sterile insect technique or population replacement. A major problem for vector mosquito mass rearing is the requirement of vertebrate blood for egg production since it poses significant costs as well as potential health hazards. Also, regulations for human and animal use as blood source can pose a significant obstacle. A completely artificial diet that supports egg production in vector mosquitoes can solve this problem. In this study, we compared different blood fractions, serum and red blood cells, as dietary protein sources for mosquito egg production. We also tested artificial diets made from commercially available blood proteins (bovine serum albumin (BSA) and hemoglobin). We found that Ae. aegypti performed vitellogenesis and produced eggs when given whole bovine blood, serum, or an artificial diet containing BSA. Conversely, egg production was impaired after feeding of the red blood cell fraction or an artificial diet containing only hemoglobin. We also found that egg viability of serum-fed mosquitoes were comparable to that of whole blood and an iron supplemented BSA meal produced more viable eggs than a meal containing BSA alone. Our results indicate that serum proteins, not hemoglobin, may replace vertebrate blood in artificial diets for mass mosquito rearing. PMID:26020000

  19. Effect of Cnidoscolus aconitifolius leaf extract on the blood glucose and insulin levels of inbred type 2 diabetic mice.

    Science.gov (United States)

    Oladeinde, F O; Kinyua, A M; Laditan, A A; Michelin, R; Bryant, J L; Denaro, F; Makinde, J M; Williams, A L; Kennedy, A P; Bronner, Y

    2007-01-01

    The effects of Cnidoscolus aconitifolius (CA) leaf extract and chlorpropamide on blood glucose and insulin levels in the inbred type 2 diabetic mice are reported. After treatment with CA, the glucose levels were measured at 0 and 2-hour intervals in experimental groups and controls. Group I received no treatment and served as control; Group II was the reference and it received chlorpropamide; Groups I-III were moderately diabetic, 100-300 mg/dL blood glucose levels while Group IV were severely diabetic (> 300 mg/dL). Groups III and IV received CA and served as test groups. There was no significant difference between the blood glucose levels at 0 and 2 hours for the control group, (P>0.23) but there were statistically significant differences for Group II (P<0.0002); Group III (P<0.002) and Group IV (P<0.0001). For moderately diabetic mice, CA and chlorpropamide decreased the glucose levels by 25.6% and 16.3% respectively while for the severely diabetic mice CA decreased the blood glucose by 43.7%. It is proposed that CA has an insulinogenic property that possibly stimulated dormant beta-cells to secrete insulin. The histopathology of several organs in the treated animals was found to differ from the expected. The islets of Langerhans for example were found to be preserved in the time frame examined. Also the liver and kidney were found to display milder pathology in the treated groups. PMID:17531147

  20. Detection and identification of antinuclear autoantibodies in the serum of normal blood donors.

    Science.gov (United States)

    de Vlam, K; De Keyser, F; Verbruggen, G; Vandenbossche, M; Vanneuville, B; D'Haese, D; Veys, E M

    1993-01-01

    The occurrence of antinuclear antibodies (ANA) in the serum of 485 healthy volunteer blood donors was assessed. Sixty two sera displayed nuclear immunofluorescence staining on Hep-2 cells using a polyvalent anti-Ig conjugate. In general, the titer of these antibodies was low (42/62 sera displaying a titer lower than or equal to 1:80). In only 23 sera were the ANA of the IgG isotype, which is the more disease-related immunoglobulin class of autoantibodies. In order to define the frequency of antibodies to extractable nuclear antigens and dsDNA within this population, sera were further analyzed by counterimmunoelectrophoresis. Western blot and the Crithidia luciliae assay. One serum displayed weak antids DNA reactivity; another serum had anti-SSA/Ro activity. On Western blot several patterns were found. They could not be identified with any of the available reference antisera. PMID:8403584

  1. Study of serum malondialdehyde and whole blood reduced glutathione in emphysema patients

    Directory of Open Access Journals (Sweden)

    Nagaraj R.Shetkar, Anand Pyati, Rajesh CS

    2013-04-01

    Full Text Available Background: Emphysema is associated with high incidence of morbidity and mortality. The imbalance between oxidants and antioxidants is thought to play an important role in the pathogenesis of Emphysema. Methods: A total number of 80 subjects comprising of 40 healthy controls and 40 Emphysema cases were studied. In all the subjects, serum levels of malondialdehyde (MDA as a biomarker of lipid peroxidation and antioxidant whole blood reduced glutathione (GSH were estimated. Results: The level of whole blood reduced glutathione was significantly decreased in emphysema patients when compared to controls. Serum MDA was significantly increased in Emphysema patients when compared to controls. Conclusion: The presence of increased systemic oxidative stress in emphysema patients seems to be associated with current active smoking and systemic inflammation. The decrease in antioxidants levels appears to be mainly a consequence of increased oxidative stress. This suggests that oxidative stress is likely to be involved in pathogenesis of emphysema.

  2. CYTOKINES IN LACRIMAL FLUID AND BLOOD SERUM: EARLY BIOMARKERS OF AGE-RELATED MACULAR DEGENERATION

    Directory of Open Access Journals (Sweden)

    O. S. Slepova

    2015-06-01

    Full Text Available The article presents results of multiplex cytokine assays in blood serum and lacrimal fluid at the initial and intermediate stages of age-related macular degeneration (AMD. Some features of local and systemic disturbances in the cytokine profile were detected in these patients. It was revealed that the initial stage of AMD was associated with elevated IL-17 levels in lacrimal fluid, along with imbalance between the local increase and systemic decrease of TGF-β1 amounts. Intermediate-stage AMD was associated with increased levels of the most cytokines assayed (except of TGF-β1 in blood serum and lacrimal fluid, thus suggesting stimulation of both pro-inflammatory and angiogenic responses, like as activation of anti-inflammatory and anti-infective factors. 

  3. Electrophoretic pattern of blood serum proteins of some of the vertebrates of Pakistan

    International Nuclear Information System (INIS)

    The electrophoretic pattern of blood serum proteins of some of the common fishes e.g. Catla catla, Cirrhina mrigala, Channa punctatus, Channa marulius, Wallago attu, Heterop-neustes fossilis; amphibia e.g., Rana tigrina, Rana cyanophlyctis, Bufo melanostictus; reptiles e.g. Varanus bengalensis, Uromastix hardwickii; birds e.g. Columba livia, Gallus domesticus, Passer domestica, Anas platyrhynchos; and mammals e.g. Homo sapiens, Mus musculus, Lepus cuniculus have been described. The mobility of proteins of blood sera has been studied over cellulose acetate paper and then a comparative pattern analysed

  4. Development of radiochemical method of analysis of binding of tritium labeled drotaverine hydrochloride with human blood serum albumin

    International Nuclear Information System (INIS)

    Full text: The albumin, being a basic functional linkage of numerous endogenous and exogenous substances is the most important protein of blood plasma. At the diseases connected to liver disfunction, collected in blood metabolite reduce connecting ability of albumino. The aim of the present research was a development of radiochemical method of determination of ability of albumin to bind the tritium labeled preparation drotaverine hydrochloride (no - spa). We had developed a micromethod of definition of connecting ability of albumin, allowing to analyse 20 mkl of blood serum. The method consists in incubation of tritium labeled drotaverine hydrochloride with blood serum in vitro, the following fractionation of serum proteins by gel - filtration on a microcolumn with Sephadex G-25, and direct measurement of the radioactivity connected to fraction of proteins of blood serum. The method has been tested on a series of blood serum of control group of healthy people and on a series of blood serum of patients with hepatitis B. We received quantitative characteristics of binding of drotaverine hydrochloride with albumin of patients with hepatitis B. It was preliminary established that binding ability of serum albumin of children with various forms of acute virus hepatitis tends to decrease in comparison with group of the control. Advantage of the developed radiochemical method is high precision and the high sensitivity of detection of infringement of binding ability of albumin. Application of tritium labeled drotaverine hydrochloride allows to measure directly levels of binding of a preparation with albumin

  5. Alteration of blood glucose levels in the rat following exposure to hyperbaric oxygen.

    Science.gov (United States)

    Eynan, Mirit; Mullokandov, Michael; Krinsky, Nitzan; Biram, Adi; Arieli, Yehuda

    2015-09-01

    Findings regarding blood glucose level (BGL) on exposure to hyperbaric oxygen (HBO) are contradictory. We investigated the influence of HBO on BGL, and of BGL on latency to central nervous system oxygen toxicity (CNS-OT). The study was conducted on five groups of rats: Group 1, exposure to oxygen at 2.5 atmospheres absolute (ATA), 90 min/day for 7 days; Group 2, exposure to oxygen once a week from 2 to 6 ATA in increments of 1 ATA/wk, for a period of time calculated as 60% of the latency to CNS-OT (no convulsions); Group 3, exposure to 6 ATA breathing a gas mixture with a pO2 of 0.21; Group 4, received 10 U/kg insulin to induce hypoglycemia before exposure to HBO; Group 5, received 33% glucose to induce hyperglycemia before exposure to HBO. Blood samples were drawn before and after exposures for measurement of BGL. No change was observed in BGL after exposure to oxygen at 2.5 ATA, 90 min/day for 7 days. BGL was significantly elevated after exposure to oxygen at 6 ATA until the appearance of convulsions, and following exposure to 4, 5, and 6 ATA without convulsions (P HBO exposure on elevation of BGL, starting at 4 ATA. This implies that BGL may serve as a marker for the generation of CNS-OT. PMID:26183474

  6. The management of blood glucose in ICU%ICU血糖监测和管理

    Institute of Scientific and Technical Information of China (English)

    钱娟; 王莹

    2012-01-01

    Hyper glycemia and hypoglycemia are associated with poor outcome of critically ill patients.No consistent evidence shows that intensive insulin strategy improves health outcomes in hospitalized patients.Because of its potentially harmful and life-threatening consequences,hypoglycemia represents the main limit to the development of a tight blood glucose control strategy in critical illness.Hypoglycemia-related neurological signs may be masked.The occurrence of hypoglycemia in critical illness,especially severe hypoglycemia,is associated with a poor prognosis.So it is necessary to closely detect glycemic variability and control the blood glucose in proper level.%ICU患儿出现高血糖和低血糖均与不良预后相关.在应激性高血糖管理中,目前并不提倡“严格血糖控制策略”,以避免低血糖等不良事件发生.在危重病患儿中低血糖相关的神经系统症状往往被掩盖,严重低血糖与不良预后、高病死率相关.因此ICU患儿密切监测血糖尤为重要,及时发现血糖异常变化予以正确处理.

  7. EFFECT OF REGULAR GARLIC INGESTION ON BODY WEIGHT AND BLOOD GLUCOSE: A CASE STUDY IN MICE

    Directory of Open Access Journals (Sweden)

    F.T. Djankpa, A. Osonuga*, J. Ekpale, C.E. Quaye, P. Otoo, O.A. Osonuga and S.K. Amoah

    2012-05-01

    Full Text Available Garlic a perennial erect plant is known to have sulphur-containing compounds that act on the hypothalamus increasing the sensitivity of the hypothalamus to leptin which alters the set point at which satiety is reached causing an organism to eat less. Nine mice (six of which were obese were used in this study and grouped into three. Groups A and B were made of 3 obese mice each whereas group C consisted of 3 non-obese mice. For group A and group C mice, 20 ml aqueous garlic extract was added to their feed daily whereas no garlic was added to the feed of group B mice. The study was carried out over a period of 44 days. The weight and blood glucose was measured weekly and the average for each group was computed. Results indicated that Group A mice recorded a reduction in mean body weight by 46.5% (p<0.05. Group B mice had significant increase in mean body weight by 46.2% (p<0.05. The blood glucose level dropped significantly by 18.5% (p<0.05 in group A mice. Garlic had weight loss and hypoglycemic effect in obese mice. These effects were absent in non-obese mice.

  8. Evaluation of the effects of Citrus sinensis seed oil on blood glucose, lipid profile and liver enzymes in rats injected with alloxan monohydrate

    Institute of Scientific and Technical Information of China (English)

    Chilaka K.C; Ifediba E.C; Ogamba J.O

    2015-01-01

    Objective: To evaluate the effects of Citrus sinensis seed oil on blood glucose, lipid profile and some liver enzymes activities in alloxan induced diabetic rats.Methods:About 120 mg/kg body weight alloxan monohydrate was injected intraperitoneally into 18 adult male albino rats weighing 180-200 g, which has been acclimatized in our laboratory for two weeks. Approximately 72 h after the alloxan injection, the rat became hyperglycaemic with blood glucose above 200 mg/dL. The diabetic rats were randomly assigned into three diabetic and one control groups of six rats each: normal control, diabetic treated with 1000 mg/kg body weight of emulsified seed oil; diabetic control, diabetic treated with 150 mg/kg body weight of metformin hydrochloride. Both controls received weight-checked solution of 4.8% v/v Tween-80 in distilled water. All injections in all groups were done intraperitoneally once daily for 28 d. The blood glucose estimation was done every week, with one touch glucometer as well as the weight checked with animal weighing balance. Lipid profiles and some liver enzymes activities (AST, ALT and ALP) were analysed using test kits and spectrophotometer. Data obtained were analyzed using One way ANOVA and post hoc test done using graph pad prism-version 6. Results: The results of this study indicated that Citrus sinensis seed oil was able to reduce blood glucose significantly (P<0.001) in the early weeks of the study when compared with both the diabetic control group and the metformin-treated group. The seed oil significantly lowered serum triglyceride, the serum LDL-cholesterol, total cholesterol and VLDL-cholesterol; the activities of all the liver enzymes assayed (P<0.05) but significantly increased the HDL-cholesterol in the diabetic oil-treated rats as compared to diabetic control (P<0.05). Conclusions: However, further studies need to be carried out to show its mechanism of action and to isolate the active ingredient in the Citrus sinensis seed oil that is

  9. Impact of admission blood glucose level on outcomes in community-acquired pneumonia in older adults

    Directory of Open Access Journals (Sweden)

    Bhattacharya RK

    2013-05-01

    Full Text Available Rajib K Bhattacharya, Jonathan D Mahnken, Sally K RiglerUniversity of Kansas School of Medicine, Kansas City, KS, USABackground: Community-acquired pneumonia (CAP is a common cause of morbidity and mortality in older adults. Although diabetes mellitus is a risk factor for pneumonia, the clinical impact of blood glucose level at the time of admission is not clear. Our goal was to examine the association between admission hyperglycemia and subsequent mortality, length of stay, and readmission outcomes in older adults with CAP.Methods: A retrospective observational study was conducted using hospital data for community-acquired pneumonia admissions in 857 persons from January 1, 2008 to December 31, 2010. We examined the effects of admission glucose level on mortality, length of stay, and 30 day readmission, adjusted for demographic factors and comorbidity.Results: The mean age of the sample was 64 years, and 51% of the subjects were female. Inpatient mortality occurred in 4.6% and the median length of stay was 5 days (interquartile range 3–9 days. Readmission within 30 days occurred in 17%. We found little impact of first glucose measures on in-hospital mortality (P = 0.94, length of stay (P = 0.95, and 30-day readmission (P = 0.56. Subjects 65 years and older trended towards higher in-hospital mortality. Older age, cancer, heart failure, and cirrhosis were associated with adverse outcomes.Conclusion: Glucose level upon admission for community-acquired pneumonia was not associated with adverse outcomes within 30 days in older adults.Keywords: community-acquired pneumonia, hyperglycemia, readmission rates, hospital mortality

  10. Effect of mastitis on macro-minerals of bovine milk and blood serum in Sudan

    OpenAIRE

    El Zubeir, I.E.M.; O.A.O. ElOwni; G.E. Mohamed

    2005-01-01

    Milk and blood serum from clinically mastitis infected, subclinically mastitis infected and healthy Friesian cows (15 samples from each of 3 groups) were evaluated for macrominerals (sodium, potassium, calcium, magnesium and phosphorus). The milk from cows infected with subclinical mastitis revealed a significant decrease in potassium (P < 0.001) and a significant increase in sodium and phosphorus content (P < 0.01). Similarly, the milk from cows with the clinical form of the disease sh...

  11. Blood pressure and serum cholesterol among male Ethiopian immigrants compared to other Israelis.

    OpenAIRE

    Green, M S; Etzion, T.; Jucha, E

    1991-01-01

    STUDY OBJECTIVE--The aim was to determine the effect of migration from a non-industrialised to an industrialised society on age related changes in blood pressure and serum cholesterol. DESIGN--The study was a comparison of major risk factors for cardiovascular disease in cross sectional surveys in two groups: Ethiopian immigrants and Israeli industrial employees. SETTING--Ethiopian immigrants were examined at Army induction centres in Israel; industrial employees were examined in the course o...

  12. Inhibition of bacterial aggregation by serum- and blood-derived proteins.

    OpenAIRE

    Malamud, D; Brown, C; Goldman, R

    1984-01-01

    Human and animal sera contain potent inhibitors of saliva-mediated aggregation of oral streptococci. The inhibitors consist of a high-molecular-weight heat-labile factor and a lower-molecular-weight heat-activated factor. The latter appears to be serum albumin. Analyses of purified blood-derived proteins indicated that several high-molecular-weight proteins (fibrinogen, fibronectin, and ferritin) were able to inhibit aggregation at low concentrations. These data suggest that high-molecular-we...

  13. Platelet Component of Hemostasis System and the Content of Homocysteine in Blood Serum in Coxarthrosis

    OpenAIRE

    Fedoseeva I.А.; Ezhov I.Y.; Tcharykova I.N.

    2011-01-01

    The aim of the investigation is to study the content of homocysteine in blood serum and the analysis of thrombocyte aggregation activity in patients suffering from degenerative-dystrophic diseases of coxofemoral joint (coxarthrosis). Materials and Methods. There have been examined 155 patients with coxarthrosis and 45 healthy men and women aged 45—65 years old. The platelet aggregation ability has been studied using adenosine diphosphate (ADP) in small doses (1.25 mkg/ml) and collagen (2....

  14. Correlations between meat quality and components of blood serum in steers fattened

    OpenAIRE

    Djagra, Ida Bagus; Wada, Hiroshi

    1980-01-01

    In fattened steers of Holstein breed correlation coefficients between factors of the carcass, and between roast grade and size of selected body parts were studied. Study of correlation between roast grade and levels of serumal components of blood taken at slaughter was carried out in the steer above mentioned. Roast grade had minus significant correlation to fat cover depth and daily gain, and plus significant correlation to hips width and thurls width. Correlations between roast grade and co...

  15. Asparagus officinalis extract controls blood glucose by improving insulin secretion and β-cell function in streptozotocin-induced type 2 diabetic rats.

    Science.gov (United States)

    Hafizur, Rahman Md; Kabir, Nurul; Chishti, Sidra

    2012-11-14

    The aim of the present study was to evaluate the anti-diabetic mechanism of Asparagus officinalis, a dietary agent used for the management of diabetes. Streptozotocin (90 mg/kg) was injected in 2-d-old Wistar rat pups to induce non-obese type 2 diabetes. After confirmation of diabetes on the 13th week, diabetic rats were treated with a methanolic extract of A. officinalis seeds (250 and 500 mg/kg per d) or glibenclamide for 28 d. After the treatment, fasting blood glucose, serum insulin and total antioxidant status were measured. The pancreas was examined by haematoxylin-eosin staining and immunostained β- and α-cells were observed using a fluorescence microscope. Treatment of the diabetic rats with the A. officinalis extract at doses of 250 and 500 mg/kg suppressed the elevated blood glucose in a dose- and time-dependent manner. The 500 mg/kg, but not 250 mg/kg, dose significantly improved serum insulin levels in the diabetic rats. The insulin:glucose ratio was significantly increased at both doses in the A. officinalis-treated rats. Both qualitative and quantitative improvements in β-cell function were found in the islets of the A. officinalis-treated rats. The extract showed potent antioxidant activity in an in vitro assay and also improved the total antioxidant status in vivo. In most cases, the efficacy of A. officinalis (500 mg/kg) was very similar to a standard anti-diabetic drug, glibenclamide. Thus, the present study suggests that A. officinalis extract exerts anti-diabetic effects by improving insulin secretion and β-cell function, as well as the antioxidant status. PMID:22221560

  16. Bedside monitoring of subcutaneous interstitial glucose in type 1 diabetic subjects using microdialysis and infrared spectrometry with optimal correlation to blood glucose concentrations

    Science.gov (United States)

    Heise, H. Michael; Damm, Uwe; Kondepati, Venkata R.; Mader, J. K.; Ellmerer, M.

    2007-02-01

    Infrared spectroscopy has been successfully employed in multi-component assays for the study of various biomedical samples. Two areas have found particular interest, i.e. in-vitro analysis in the clinical laboratory and point-of-care applications. With regard to the latter field, in-vivo blood glucose monitoring is an important topic for improving glycemic control in critically ill patients with non-adequate blood glucose regulation, similar to the situation faced for diabetic patients. For such application, a continuously operated mid-infrared spectroscopic system in combination with a subcutaneously implanted microdialysis probe and coupled by micro-fluidics has been developed. Using the dialysis process, the interstitial fluid matrix can be significantly simplified, since high molecular mass compounds such as proteins are separated. However, the micro-dialysis recovery rate is variable over time, so that a simultaneous determination of this parameter was implemented using the losses of an acetate marker from the perfusate across the dialysis membrane. Clinical measurements were carried out on type 1 diabetic subjects, with experiments lasting up to 28 hours. The concentrations of glucose, acetate and other components in the dialysates from interstitial body fluids were investigated. Two different multivariate calibration strategies, i.e. partial least squares (PLS) and classical least squares (CLS) regressions were applied. The results led to excellent correlation of the subcutaneous interstitial concentrations with those of laboratory blood glucose readings. Clarke-Error-Grid evaluations were employed for assessing the clinical applicability of the method.

  17. A paper disk equipped with graphene/polyaniline/Au nanoparticles/glucose oxidase biocomposite modified screen-printed electrode: toward whole blood glucose determination.

    Science.gov (United States)

    Kong, Fen-Ying; Gu, Sai-Xi; Li, Wei-Wei; Chen, Ting-Ting; Xu, Qin; Wang, Wei

    2014-06-15

    In this work, a convenient, fast, low cost, small sample volume and in situ detection of glucose in human whole blood has been developed by using a disposable screen-printed carbon electrode (SPCE) coupled with a paper disk. To perform the assay, the SPCE was modified with graphene/polyaniline/Au nanoparticles/glucose oxidase (Gr/PANI/AuNPs/GOD) biocomposite and then covered by a paper disk impregnated with the sample. After introducing PBS on the paper disk, the electrochemical measurement was carried out. The assay was based on measuring the current decrease of flavin adenine dinucleotide (FAD) in GOD provoked by the enzyme-substrate reaction using differential pulse voltammetry (DPV). The analytical performance was comparable to conventional methods, and covered the full range of clinically relevant concentrations of glucose in whole blood. This new paper-based electrochemical glucose sensor shows promise in applying point-of-care (POC) device in whole blood tests, and particularly being appropriate for use in the developing world and in resource-limited settings. PMID:24469540

  18. Effect of different types of stress on adrenal gland parameters and adrenal hormones in the blood serum of male Wistar rats

    Directory of Open Access Journals (Sweden)

    Adžić M.

    2009-01-01

    Full Text Available In the present study, we examined gross changes in the mass of whole adrenal glands and that of the adrenal cortex and medulla in mature male Wistar rats subjected to three different stress types: acute, chronic, and combined, i.e., chronic followed by acute stress. These parameters were correlated with adrenal activity as judged from serum levels of corticosterone and catecholamine, respectively, as well as with serum levels of ACTH and glucose. Under all three conditions, we observed bilaterally asymmetric and stress-type-independent hypertrophy of whole adrenals, as well as adrenal cortices and medullas. Under acute and combined stress, adrenal hypertrophy was followed by increase of adrenal hormones in the blood serum. However, under chronic stress, both cortical and medullar activities as judged from low or unaltered levels of the respective hormones and glucose were compromised and disconnected from the input signal of ACTH. Since all of the studied adrenal activities could be restored by subsequent acute stress, it is concluded that chronic isolation can be viewed as partly maladaptive stress with characteristics resembling stress resistance rather than the stress exhaustion stage of the general adaptation syndrome.

  19. Analysis of fasting blood glucose and glucose tolerance in 1 157 subjects%1157例体检者空腹血糖及糖耐量分析

    Institute of Scientific and Technical Information of China (English)

    王进

    2012-01-01

    Objective To analyze the fasting blood glucose(FBG) and glucose tolerance in 1 157 subjects accepting health examination to understand level and metabolism of blood glucose of local residents. Methods Adopting the method of population proportional sampling(PPS) , 1 157 subjects of all residents accepting health examination were randomly selected and divided into three groups according to economic conditions for the detection of FBG, among which 926 cases were detected for glucose tolerance by determination of blood glucose level 2 hours after oral intaking of 75 g glucose. Results 198 cases with high level of blood glucose and 117 cases with impaired glucose tolerance, accounting for 12. 63% , were demonstrated, and the prevalence of diabetes mellitus was 4. 86%. Conclusion The situation and prevalence of diabetes mellitus was serious in this region. Prevention of diabetes mellitus should be carried out from unban with better economic condition to other regions until the whole region.%目的 对1 157例体检者空腹血糖及糖耐量分析,掌握该区居民的血糖及其代谢情况.方法 采用人口成比例抽样(PPS)的方法,按经济条件分成3个层次,随机抽取1 157例研究对象采用葡萄糖氧化酶法测定空腹血糖,其中926例口服75 g无水葡萄糖2 h后测量血糖.结果 共计检出高血糖患者198例,检出糖耐量受损者117例,占12.63%,糖尿病患病率则为4.86%.结论 该区糖尿病患者及潜在者形势严峻,该区当前糖尿病防治应以经济较好的市区为重点向开发区辐射直至全区.

  20. Characterization of monoclonal gammopathy of undetermined significance by calorimetric analysis of blood serum proteome.

    Directory of Open Access Journals (Sweden)

    Francisca Barceló

    Full Text Available Monoclonal gammopathy of undetermined significance (MGUS is a premalignant proliferative disorder that may progress to multiple myeloma, a malignant plasma cell neoplasia. We evaluated differential scanning calorimetry (DSC as an experimental tool for differentiating serum samples of MGUS patients from healthy individuals. DSC thermograms can be used for monitoring changes in the serum proteome associated with MGUS. MGUS patients showed great variability in serum thermogram characteristics, which depended on the IgG, IgA or IgM isotypes and/or the κ or λ light chains. Thermogram feature parameters distinguished patients with MGUS from healthy people. Serum samples, named as non-MGUS, were also collected from patients with subjacent immunological pathologies who were discarded of having MGUS through serum immunofixation. They were used to verify the sensitivity of DSC for discriminating MGUS from related blood dyscrasias. Only some DSC thermogram feature parameters differentiated, to a lesser extent, between MGUS and non-MGUS individuals. We contemplate DSC as a tool for early diagnosis and monitoring of MGUS.