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Sample records for blood count thromboxane

  1. Understanding Blood Counts

    Science.gov (United States)

    ... Lab and Imaging Tests Understanding Blood Counts Understanding Blood Counts Understanding Blood Counts SHARE: Print Glossary Blood cell counts give ... your blood that's occupied by red cells. Normal Blood Counts Normal blood counts fall within a range ...

  2. White Blood Cell Count

    Science.gov (United States)

    ... limited. Home Visit Global Sites Search Help? White Blood Cell Count Share this page: Was this page helpful? ... Count; Leukocyte Count; White Count Formal name: White Blood Cell Count Related tests: Complete Blood Count , Blood Smear , ...

  3. Blood Count Tests

    Science.gov (United States)

    Your blood contains red blood cells (RBC), white blood cells (WBC), and platelets. Blood count tests measure the number and types of cells in your blood. This helps doctors check on your overall health. ...

  4. [Blood Count Specimen].

    Science.gov (United States)

    Tamura, Takako

    2015-12-01

    The circulating blood volume accounts for 8% of the body weight, of which 45% comprises cellular components (blood cells) and 55% liquid components. We can measure the number and morphological features of blood cells (leukocytes, red blood cells, platelets), or count the amount of hemoglobin in a complete blood count: (CBC). Blood counts are often used to detect inflammatory diseases such as infection, anemia, a bleeding tendency, and abnormal cell screening of blood disease. This count is widely used as a basic data item of health examination. In recent years, clinical tests before consultation have become common among outpatient clinics, and the influence of laboratory values on consultation has grown. CBC, which is intended to count the number of raw cells and to check morphological features, is easily influenced by the environment, techniques, etc., during specimen collection procedures and transportation. Therefore, special attention is necessary to read laboratory data. Providing correct test values that accurately reflect a patient's condition from the laboratory to clinical side is crucial. Inappropriate medical treatment caused by erroneous values resulting from altered specimens should be avoided. In order to provide correct test values, the daily management of devices is a matter of course, and comprehending data variables and positively providing information to the clinical side are important. In this chapter, concerning sampling collection, blood collection tubes, dealing with specimens, transportation, and storage, I will discuss their effects on CBC, along with management or handling methods.

  5. High Red Blood Cell Count

    Science.gov (United States)

    Symptoms High red blood cell count By Mayo Clinic Staff A high red blood cell count is an increase in oxygen-carrying cells in your bloodstream. Red blood cells transport oxygen from your lungs to tissues throughout ...

  6. Low White Blood Cell Count

    Science.gov (United States)

    Symptoms Low white blood cell count By Mayo Clinic Staff A low white blood cell count (leukopenia) is a decrease in disease-fighting cells ( ... a decrease in a certain type of white blood cell (neutrophil). The definition of low white blood cell ...

  7. A native whole blood test for the evaluation of blood-surface interaction: determination of thromboxane production.

    Science.gov (United States)

    Mantovani, E; Marconi, W; Cebo, B; Togna, A R; Togna, G; Caprino, L

    1984-05-01

    The method developed to evaluate the hemocompatibility of artificial materials involves the determination of thromboxane production during the clotting of rabbit blood, in test tubes of different materials. The concentration of serum TXB2 obtained after incubation of whole blood in glass test tubes, for 40 min at 37 degrees C, averaged 416.8 +/- 23.3 ng/ml (mean +/- SE). Polymethylpentene, recognised as having a relatively poor blood compatibility, elicited 309.5 +/- 17.2 ng/ml of serum TXB2, while silicone and Avcothane, considered of better hemocompatibility, showed thromboxane levels of 276.2 +/- 28.2 and 222.9 +/- 31.5 ng/ml, respectively. These values validate the usefulness of the proposed method as a preliminary in vitro screening test of artificial materials intended for biomedical application.

  8. White blood cell counting system

    Science.gov (United States)

    1972-01-01

    The design, fabrication, and tests of a prototype white blood cell counting system for use in the Skylab IMSS are presented. The counting system consists of a sample collection subsystem, sample dilution and fluid containment subsystem, and a cell counter. Preliminary test results show the sample collection and the dilution subsystems are functional and fulfill design goals. Results for the fluid containment subsystem show the handling bags cause counting errors due to: (1) adsorption of cells to the walls of the container, and (2) inadequate cleaning of the plastic bag material before fabrication. It was recommended that another bag material be selected.

  9. Low white blood cell count and cancer

    Science.gov (United States)

    ... this page: //medlineplus.gov/ency/patientinstructions/000675.htm Low white blood cell count and cancer To use ... high blood pressure, or seizures Continue Reading How Low is too Low? When your blood is tested, ...

  10. Complete Blood Count and Retinal Vessel Calibers

    OpenAIRE

    Gerald Liew; Jie Jin Wang; Elena Rochtchina; Tien Yin Wong; Paul Mitchell

    2014-01-01

    OBJECTIVE: The influence of hematological indices such as complete blood count on microcirculation is poorly understood. Retinal microvasculature can be directly visualized and vessel calibers are associated with a range of ocular and systemic diseases. We examined the association of complete blood count with retinal vessel calibers. METHODS: Cross-sectional population-based Blue Mountains Eye Study, n = 3009, aged 49+ years. Complete blood count was measured from fasting blood samples taken ...

  11. French maritime pine bark extract Pycnogenol reduces thromboxane generation in blood from diabetic male rats.

    Science.gov (United States)

    Nocun, Marek; Ulicna, Olga; Muchova, Jana; Durackova, Zdenka; Watala, Cezary

    2008-03-01

    The protective effect of Pycnogenol against cardiovascular diseases was clearly demonstrated. Nevertheless, little is known about its antithrombotic effect, especially in diabetes associated with enhanced thromboxane synthesis leading to severe vascular complications. Therefore, the main purpose of our study was to evaluate the effect of long-term Pycnogenol intake on synthesis of prothrombotic thromboxane A(2) (TXA(2)) in animal model of insulin-dependent diabetes. The levels of main plasma TXA(2) metabolite, thromboxane B(2) (TXB(2)), were assessed by enzyme-linked immunosorbent assay. Diabetes was induced in Wistar male rats by single injection of streptozotocin, resulting after 8 weeks in significant body weight reduction, increased plasma glucose concentrations, and decreased plasma C-peptide levels, compared to non-diabetic animals. There was no significant reduction of plasma glucose concentrations after Pycnogenol ingestion. It was found, however, that daily administration of either Pycnogenol (5mg/kg b.wt.) or acetylsalicylic acid (ASA, 10mg/kg b.wt.) significantly reduced plasma TXB(2) concentrations, and this inhibitory effect was higher in the latter case. Nonetheless, simultaneous administration of Pycnogenol and ASA did not improve effectiveness of ASA-mediated decrease in TXB(2) generation. The results of the present study suggest that Pycnogenol might have a beneficial antithrombotic effect when administered alone or as a supplementation of standard antiplatelet therapy in diabetic patients.

  12. [Myocardial ischemia during exertion. Correlations between blood levels of thromboxane B2 and changes in coronary flow and resistance].

    Science.gov (United States)

    De Servi, S; Vidale, E; Mussini, A; Cafiso, A; Gavazzi, A; Falcone, C; Bramucci, E; Angoli, L; Ferrario, M; Ghio, S

    1985-01-01

    Platelet activation, with the subsequent generation of Thromboxane (Tx) A2, has been implied as a possible cause of resting as well as exercise induced myocardial ischemia. To verify the latter hypothesis, we measured the exercise release of TxB2, the stable metabolite of TxA2, in 9 patients with exertional angina and left anterior descending coronary artery disease. Three of the patients also suffered from angina at rest, due to coronary vasospasm. The great cardiac vein flow, venous efflux from the myocardial territory supplied by the left anterior descending, was determined by the thermodilution technique in the basal conditions, at peak exercise when angina and/or significant ST changes occurred, and 20 min after exercise. Simultaneous blood samples were drawn from the great cardiac vein and a peripheral artery for TxB2 measurements. Regional coronary resistances were calculated as the ratio of mean arterial pressure and coronary flow. At peak exercise the great cardiac vein flow increased and regional coronary resistances decreased in all patients, except in one who showed exercise induced coronary spasm. An increase in TxB2 release was found in 3 patients, a decrease in 3, while the remaining 3 patients did not show significant changes. After exercise the great cardiac vein flow and regional coronary resistances returned to control values in all, whereas both great cardiac vein and arterial TxB2 levels were increased in 6 patients. Our data show that no apparent relation exists between exercise-induced changes in coronary resistances and generation of TxB2.(ABSTRACT TRUNCATED AT 250 WORDS)

  13. 21 CFR 864.6160 - Manual blood cell counting device.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Manual blood cell counting device. 864.6160... (CONTINUED) MEDICAL DEVICES HEMATOLOGY AND PATHOLOGY DEVICES Manual Hematology Devices § 864.6160 Manual blood cell counting device. (a) Identification. A manual blood cell counting device is a device used...

  14. Trapping cells in paper for white blood cell count.

    Science.gov (United States)

    Zhang, Yi; Bai, Jianhao; Wu, Hong; Ying, Jackie Y

    2015-07-15

    White blood cell count is an important indicator of each individual's health condition. An abnormal white blood cell count usually results from an infection, cancer, or other conditions that trigger systemic inflammation responses. White blood cell count also provides predictive information on the incidence of cardiovascular diseases and Type 2 diabetes. Therefore, monitoring white blood cell count on a regular basis can potentially help individuals to take preventive measures and improve healthcare outcomes. Currently, white blood cell count is primarily conducted in centralized laboratories, and it requires specialized equipment and dedicated personnel to perform the test and interpret the results. So far there has been no rapid test that allows white blood cell count in low-resource settings. In this study, we have demonstrated a vertical flow platform that quantifies white blood cells by trapping them in the paper. White blood cells were tagged with gold nanoparticles, and flowed through the paper via a small orifice. The white blood cell count was determined by measuring the colorimetric intensity of gold nanoparticles on the surface of white blood cells that were trapped in the paper mesh. Using this platform, we were able to quantify white blood cells in 15 μL of blood, and visually differentiate the abnormal count of white blood cells from the normal count. The proposed platform enabled rapid white blood cell count in low resource settings with a small sample volume requirement. Its low-cost, instrument-free operations would be attractive for point-of-care applications.

  15. Foetal vascular responses to thromboxane receptor blockade

    Directory of Open Access Journals (Sweden)

    B. A. Meyer

    1992-01-01

    Full Text Available We hypothesized that foetal administration of SQ-29,548, a putative thromboxane receptor blocker, would prevent foeto–placental vasoconstriction produced by the thromboxane mimic U46619. Arterial blood gases, continuous monitoring of maternal and foetal heart rates and blood pressures were performed in chronically catheterized pregnant ewes. Foetal blood flows and vascular resistance were determined with radioactive microspheres. SQ-29,548 effectively blocked the expected vasoconstrictive effects of thromboxane. However, prolonged infusion of SQ-29,548 resulted in significant decreases in umbilical–placental blood flow and foetal mean arterial pressure. This was accompanied by a respiratory acidemia. Potential therapy for the vasoconstrictive disorders of pregnancy with SQ-29,548 awaits further investigation of its intrinsic vasoactive properties in the umbilical–placental vasculature.

  16. Microfluidic Cytometer for Complete Blood Count Analysis Project

    Data.gov (United States)

    National Aeronautics and Space Administration — RMD proposes to develop a MEMS based complete blood count (CBC) instrument that can be used aboard a spacecraft. We will produce a microfluidic scale combination...

  17. Microfluidic Cytometer for Complete Blood Count Analysis Project

    Data.gov (United States)

    National Aeronautics and Space Administration — We will fabricate and test microfluidic designs for a micro-electromechanical system based complete blood count (CBC) analysis in separate modules and integrate them...

  18. Automated counting of white blood cells in synovial fluid.

    NARCIS (Netherlands)

    R. de Jonge (Robert); R.W. Brouwer (Reinoud); M. Smit (Marij); M. de Frankrijker-Merkestijn; R.J. Dolhain; J.M.W. Hazes (Mieke); A.W. van Toorenenbergen (Albert); J. Lindemans (Jan)

    2004-01-01

    textabstractOBJECTIVES: To evaluate the performance of automated leucocyte (white blood cell; WBC) counting by comparison with manual counting. METHODS: The number of WBC was determined in heparinized synovial fluid samples by the use of (i) a standard urine cytometer (Kova) and a

  19. Complete blood count reference intervals for healthy Han Chinese adults.

    Directory of Open Access Journals (Sweden)

    Xinzhong Wu

    Full Text Available Complete blood count (CBC reference intervals are important to diagnose diseases, screen blood donors, and assess overall health. However, current reference intervals established by older instruments and technologies and those from American and European populations are not suitable for Chinese samples due to ethnic, dietary, and lifestyle differences. The aim of this multicenter collaborative study was to establish CBC reference intervals for healthy Han Chinese adults.A total of 4,642 healthy individuals (2,136 males and 2,506 females were recruited from six clinical centers in China (Shenyang, Beijing, Shanghai, Guangzhou, Chengdu, and Xi'an. Blood samples collected in K2EDTA anticoagulant tubes were analyzed. Analysis of variance was performed to determine differences in consensus intervals according to the use of data from the combined sample and selected samples.Median and mean platelet counts from the Chengdu center were significantly lower than those from other centers. Red blood cell count (RBC, hemoglobin (HGB, and hematocrit (HCT values were higher in males than in females at all ages. Other CBC parameters showed no significant instrument-, region-, age-, or sex-dependent difference. Thalassemia carriers were found to affect the lower or upper limit of different RBC profiles.We were able to establish consensus intervals for CBC parameters in healthy Han Chinese adults. RBC, HGB, and HCT intervals were established for each sex. The reference interval for platelets for the Chengdu center should be established independently.

  20. Absolute counting of neutrophils in whole blood using flow cytometry.

    Science.gov (United States)

    Brunck, Marion E G; Andersen, Stacey B; Timmins, Nicholas E; Osborne, Geoffrey W; Nielsen, Lars K

    2014-12-01

    Absolute neutrophil count (ANC) is used clinically to monitor physiological dysfunctions such as myelosuppression or infection. In the research laboratory, ANC is a valuable measure to monitor the evolution of a wide range of disease states in disease models. Flow cytometry (FCM) is a fast, widely used approach to confidently identify thousands of cells within minutes. FCM can be optimised for absolute counting using spiked-in beads or by measuring the sample volume analysed. Here we combine the 1A8 antibody, specific for the mouse granulocyte protein Ly6G, with flow cytometric counting in straightforward FCM assays for mouse ANC, easily implementable in the research laboratory. Volumetric and Trucount™ bead assays were optimized for mouse neutrophils, and ANC values obtained with these protocols were compared to ANC measured by a dual-platform assay using the Orphee Mythic 18 veterinary haematology analyser. The single platform assays were more precise with decreased intra-assay variability compared with ANC obtained using the dual protocol. Defining ANC based on Ly6G expression produces a 15% higher estimate than the dual protocol. Allowing for this difference in ANC definition, the flow cytometry counting assays using Ly6G can be used reliably in the research laboratory to quantify mouse ANC from a small volume of blood. We demonstrate the utility of the volumetric protocol in a time-course study of chemotherapy induced neutropenia using four drug regimens.

  1. Adjusting MtDNA Quantification in Whole Blood for Peripheral Blood Platelet and Leukocyte Counts

    Science.gov (United States)

    Gonzalez-Lazaro, Monica; Moreno-Loshuertos, Raquel; Fernandez-Silva, Patricio; Enriquez, Jose Antonio; Laclaustra, Martin

    2016-01-01

    Alterations of mitochondrial DNA copy number (mtDNAcn) in the blood (mitochondrial to nuclear DNA ratio) appear associated with several systemic diseases, including primary mitochondrial disorders, carcinogenesis, and hematologic diseases. Measuring mtDNAcn in DNA extracted from whole blood (WB) instead of from peripheral blood mononuclear cells or buffy coat may yield different results due to mitochondrial DNA present in platelets. The aim of this work is to quantify the contribution of platelets to mtDNAcn in whole blood [mtDNAcn(WB)] and to propose a correction formula to estimate leukocytes' mtDNAcn [mtDNAcn(L)] from mtDNAcn(WB). Blood samples from 10 healthy adults were combined with platelet-enriched plasma and saline solution to produce artificial blood preparations. Aliquots of each sample were combined with five different platelet concentrations. In 46 of these blood preparations, mtDNAcn was measured by qPCR. MtDNAcn(WB) increased 1.07 (95%CI 0.86, 1.29; p<0.001) per 1000 platelets present in the preparation. We proved that leukocyte count should also be taken into account as mtDNAcn(WB) was inversely associated with leukocyte count; it increased 1.10 (95%CI 0.95, 1.25, p<0.001) per unit increase of the ratio between platelet and leukocyte counts. If hematological measurements are available, subtracting 1.10 the platelets/leukocyte ratio from mtDNAcn(WB) may serve as an estimation for mtDNAcn(L). Both platelet and leukocyte counts in the sample are important sources of variation if comparing mtDNAcn among groups of patients when mtDNAcn is measured in DNA extracted from whole blood. Not taking the platelet/leukocyte ratio into account in whole blood measurements, may lead to overestimation and misclassification if interpreted as leukocytes' mtDNAcn. PMID:27736919

  2. Ancestry informative markers and complete blood count parameters in Brazilian blood donors

    Directory of Open Access Journals (Sweden)

    Gabriela E. S. Felix

    Full Text Available A complete blood count is very useful in clinical diagnoses when reference ranges are well established for the population. Complete blood counts and allele frequencies of Ancestry Informative Markers (AIMs were analyzed in Brazilians with the aim of characterizing the hematological values of an admixed population. Positive associations were observed between gender and neutrophils, monocytes, eosinophils, erythrocytes, hemoglobin, hematocrit, MCV, MCHC and platelet counts. No significant differences were found for age, alcohol consumption, educational status, ethnicity, smoking in respect to the complete blood count values. In general, men had higher red blood cell values, while women had higher values for white blood cells and platelets. The study of the population was highly heterogeneous with mean proportions (± SE of African, European and Amerindian ancestry being 49.0 ± 3.0%, 44.0 ± 9.0% and 7.0 ± 9.0%, respectively. Amerindian ancestry showed limited contribution to the makeup of the population, but estimated ancestral proportions were statistically significant (r = 0.9838; P<0.001. These hematologic values are similar to Afro-Americans, another admixed population.

  3. Under-filled blood collection tubes containing K2EDTA as anticoagulant are acceptable for automated complete blood counts, white blood cell differential, and reticulocyte count.

    Science.gov (United States)

    Xu, M; Robbe, V A; Jack, R M; Rutledge, J C

    2010-10-01

    Current laboratory standards from Clinical Laboratory Standards Institute (CLSI) and manufacturer's (Becton Dickinson) data indicate that under-filling K(2)EDTA blood collection tubes can result in erroneous hematology values. To accommodate under-filled tubes and reduce collection volumes while optimizing our automation, we explored the acceptable limit of under-filled tubes for hematology values. We collected 8.0 ml of blood from 30 normal adult volunteers. Each donation was aliquoted in the following volumes: 4.0, 2.0, 1.0, 0.5 ml x 2. These samples were analyzed within 1 h of blood collection on Sysmex XE-2100 (Sysmex America Inc., Mundelein, IL, USA) for complete blood count, reticulocyte, and white blood cell differentials. Results of the under-filled tubes were compared to those of the standard volume. The Deming regression analysis show excellent correlation for all parameters between each under-filled blood collection volume compared to a standard 4 ml volume. The Bland and Altman analysis shows good agreement between both 1.0 and 2.0 ml compared to a 4.0 ml volume. The 0.5 ml compared to a 4.0 ml volume, however, shows increased variation on many parameters. In addition all three collection volumes show negative bias compared to the standard volume for platelet count, but the difference is considered insignificant with a percent difference of 5.5%, 3.2%, and 1.5% for 0.5, 1.0, and 2.0 ml collection volume respectively. Finally for 0.5 ml collection volume we noticed a low level of false positive flagging rate for white blood cell. Acceptable complete blood count values of under-filled powdered K(2)EDTA tubes can be obtained with as little as 1.0 ml of blood.

  4. [Verification of complete blood cell count (CBC) data from heparinized blood gas samples].

    Science.gov (United States)

    Sakoguchi, Takafumi; Fujii, Seiji; Inuzumi, Koji; Kaminoh, Yoshiroh; Hirose, Munetaka; Masaki, Mitsuru; Koshiba, Masahiro

    2014-02-01

    Complete blood cell count (CBC) data from heparinized blood gas (H-Gas) samples were verified with primary focus on the platelet count (PLT). When a part of H-Gas sample was taken to a separation tube from the blood collection syringe and CBC of the sample in the separation tube was repeatedly measured (Procedure 1), the PLT from 5 samples relative to that obtained immediately after the separation was gradually reduced to 72.6-94.2% during serial measurements (every 5 minutes, up to 30 minutes). The change in the scattergram pattern suggested that this PLT decrease was due to the formation of platelet clumps. The white blood cell count (WBC), red blood cell count (RBC), hemoglobin (Hb) and hematocrit (Ht) values did not significantly change during the repeated measurements. On the other hand, PLT was significantly improved to 96.8-99.8% when the H-Gas sample was kept in the blood collection syringe so as to minimizing the exposure to the air, and the sample for the measurement from H-Gas was taken every time to separation tube from the syringe, followed by CBC measurement without delay (Procedure 2). In addition, while there were significant variations (CV: 11.8-18.2%) in PLT reproducibility among H-Gas samples by Procedure 1, measurements utilizing the Procedure 2 resulted in much smaller variations (CV: 2.2-3.7%). Thus the CBC data obtained from H-Gas samples were equivalent to those from EDTA samples when the Procedure 2 was applied. These data suggest that H-Gas samples can be used for the accurate CBC measurement, including PLT, by applying the Procedure 2.

  5. Relationship of blood and milk cell counts with mastitic pathogens in Murrah buffaloes

    Directory of Open Access Journals (Sweden)

    C. Singh

    2010-02-01

    Full Text Available The present study was undertaken to see the effect of mastitic pathogens on the blood and milk counts of Murrah buffaloes. Milk and blood samples were collected from 9 mastitic Murrah buffaloes. The total leucocyte Counts (TLC and Differential leucocyte counts (DLC in blood were within normal range and there was a non-significant change in blood counts irrespective of different mastitic pathogens. Normal milk quarter samples had significantly (P<0.01 less Somatic cell counts (SCC. Lymphocytes were significantly higher in normal milk samples, whereas infected samples had a significant increase (P<0.01 in milk neutrophils. S. aureus infected buffaloes had maximum milk SCC, followed by E. coli and S. agalactiae. Influx of neutrophils in the buffalo mammary gland was maximum for S. agalactiae, followed by E.cli and S. aureus. The study indicated that level of mastitis had no affect on blood counts but it influenced the milk SCC of normal quarters.

  6. Counting

    Institute of Scientific and Technical Information of China (English)

    许有国

    2005-01-01

    Most people began to count in tens because they had ten fingers on their hands. But in some countries, people counted on one hand and used the three parts of their four fingers. So they counted in twelves, not in tens.

  7. Impact of valproates on haemostasis and blood cell count in children

    OpenAIRE

    Igrutinović Zoran; Obradović Slobodan; Vuletić Biljana; Marković Slavica

    2008-01-01

    INTRODUCTION Epilepsy is a highly prevalent disease affecting 0.5-1.5% of the world's population. One of the most frequently used antiepileptics are valproates. These medicines show a negative impact on haemostasis and peripheral blood count. OBJECTIVE The objective of the study was to examine the negative impact of valproates on haemostasis and peripheral blood count in children and to analyse whether these disturbances were dependent on the dosage of valproates and drug level in blood. METH...

  8. Association between absolute blood eosinophil count and CKD stages among cardiac patients.

    Science.gov (United States)

    Ishii, Rui; Fujita, Shu-Ichi; Kizawa, Shun; Sakane, Kazushi; Morita, Hideaki; Ozeki, Michishige; Sohmiya, Koichi; Hoshiga, Masaaki; Ishizaka, Nobukazu

    2016-02-01

    Elevated eosinophil count was shown to be associated with the development of cholesterol embolization syndrome, a potentially life-threatening condition, after catheter-based procedures. We investigated the association between stages of chronic kidney disease (CKD) and the absolute eosinophil count (AEC) among cardiac patients. CKD stages were determined solely on the estimated glomerular filtration rate or requirement for hemodialysis. Eosinophilia is defined as an eosinophil count exceeding 500/μL. A total of 1022 patients were enrolled in the current study, and eosinophil counts (/μL) in the first through fourth eosinophil count quartiles were blood pressure, and total white blood cell count. Similarly, after adjustment for the same variables, eosinophilia was associated with severe renal dysfunction with an odds ratio of 2.60 (95 % confidence interval, 1.08-6.26, P count was positively associated with higher CKD stages among cardiology patients, some fraction of which might be related to subclinical cholesterol embolization.

  9. Association of the blood eosinophil count with hematological malignancies and mortality

    DEFF Research Database (Denmark)

    Andersen, Christen L; Siersma, Volkert D; Hasselbalch, Hans C;

    2015-01-01

    Blood eosinophilia (≥0.5x10(9) /l) may be an early sign of hematological malignancy. We investigated associations between levels of blood eosinophils and risks of hematological malignancies and mortality in order to provide clinically derived cut-offs for referral to specialist hematology care....... From the Copenhagen Primary Care Differential Count (CopDiff) Database, we identified 356,196 individuals with at least one differential cell count encompassing the eosinophil count during 2000-2007 and matched these laboratory data with Danish nationwide health registers. We used multivariable...... logistic regression to calculate odds ratios (ORs) for the 4-year incidences of hematological malignancies and mortality between the eosinophil counts and a reference count of 0.16x10(9) /l which was the median eosinophil count in our data. Risks of hematological malignancies and mortality increased above...

  10. Blood eosinophil count and exacerbations in severe chronic obstructive pulmonary disease after withdrawal of inhaled corticosteroids

    DEFF Research Database (Denmark)

    Watz, Henrik; Tetzlaff, Kay; Wouters, Emiel F M

    2016-01-01

    BACKGROUND: Blood eosinophil counts might predict response to inhaled corticosteroids (ICS) in patients with chronic obstructive pulmonary disease (COPD) and a history of exacerbations. We used data from the WISDOM trial to assess whether patients with COPD with higher blood eosinophil counts would...... to receive either continued or reduced ICS over 12 weeks. We did a post-hoc analysis after complete ICS withdrawal (months 3-12) to compare rate of exacerbations and time to exacerbation outcomes on the basis of blood eosinophil subgroups of increasing cutoff levels. The WISDOM trial is registered...... at ClinicalTrials.gov, number NCT00975195. FINDINGS: In the 2296 patients receiving treatment after ICS withdrawal, moderate or severe exacerbation rate was higher in the ICS-withdrawal group versus the ICS-continuation group in patients with eosinophil counts (out of total white blood cell count) of 2...

  11. White blood cell count and risk of incident atrial fibrillation (from the Framingham Heart Study)

    NARCIS (Netherlands)

    Rienstra, Michel; Sun, Jenny X.; Magnani, Jared W.; Sinner, Moritz F.; Lubitz, Steven A.; Sullivan, Lisa M.; Ellinor, Patrick T.; Benjamin, Emelia J.

    2012-01-01

    Several studies have reported that inflammatory markers are associated with atrial fibrillation (AF). The white blood cell (WBC) count is a widely available and broadly used marker of systemic inflammation. We sought to investigate the association between an increased WBC count and incident AF and w

  12. Polycystic ovary syndrome and the peripheral blood white cell count.

    LENUS (Irish Health Repository)

    Herlihy, A C

    2012-02-01

    This retrospective cross-sectional study examined if the white cell count (WCC) is increased in women with polycystic ovary syndrome (PCOS) and if so, is it due to PCOS or to the associated obesity? Body mass index (BMI) was calculated and body composition was measured using bioelectrical impedance analysis. Of the 113 women studied, 36 had PCOS and 77 did not. The mean WCC was higher in the PCOS group compared with the non-PCOS group (8.9 x 10(9)\\/l vs 7.4 x 10(9)\\/l p = 0.002). This increase was due to a higher neutrophil count (5.6 x 10(9)\\/l vs 4.3 x 10(9)\\/l; p = 0.003). There was a leucocytosis (WCC >11 x 10(9)\\/l) present in 19% of the PCOS group compared with 1% in the non-PCOS group (p < 0.001). The neutrophil count was abnormally high (>7.7 x 10(9)\\/l) in 14% of the PCOS group compared with 4% in the non-PCOS group (p < 0.001). On regression analysis, however, the only independent variable which explained both the increased WCC and the increased neutrophil count was PCOS. We found that PCOS is associated with an increased WCC due to increased neutrophils, which supports the evidence that PCOS is associated with low-grade inflammation. The increase appears to be due to the underlying PCOS, and not to the increased adiposity associated with PCOS.

  13. Electrochemical Red Blood Cell Counting: One at a Time.

    Science.gov (United States)

    Sepunaru, Lior; Sokolov, Stanislav V; Holter, Jennifer; Young, Neil P; Compton, Richard G

    2016-08-08

    We demonstrate that the concentration of a red blood cell solution under physiological conditions can be determined by electrochemical voltammetry. The magnitude of the oxygen reduction currents produced at an edge-plane pyrolytic graphite electrode was diagnosed analytically at concentrations suitable for a point-of-care test device. The currents could be further enhanced when the solution of red blood cells was exposed to hydrogen peroxide. We show that the enhanced signal can be used to detect red blood cells at a single entity level. The method presented relies on the catalytic activity of red blood cells towards hydrogen peroxide and on surface-induced haemolysis. Each single cell activity is expressed as current spikes decaying within a few seconds back to the background current. The frequency of such current spikes is proportional to the concentration of cells in solution.

  14. Association of the blood eosinophil count with hematological malignancies and mortality.

    Science.gov (United States)

    Andersen, Christen L; Siersma, Volkert D; Hasselbalch, Hans C; Vestergaard, Hanne; Mesa, Ruben; Felding, Peter; Olivarius, Niels D F; Bjerrum, Ole W

    2015-03-01

    Blood eosinophilia (≥0.5 × 10(9) /l) may be an early sign of hematological malignancy. We investigated associations between levels of blood eosinophils and risks of hematological malignancies and mortality in order to provide clinically derived cut-offs for referral to specialist hematology care. From the Copenhagen Primary Care Differential Count (CopDiff) Database, we identified 356,196 individuals with at least one differential cell count encompassing the eosinophil count during 2000-2007 and matched these laboratory data with Danish nationwide health registers. We used multivariable logistic regression to calculate odds ratios (ORs) for the 4-year incidences of hematological malignancies and mortality between the eosinophil counts and a reference count of 0.16 × 10(9) /l which was the median eosinophil count in our data. Risks of hematological malignancies and mortality increased above the median eosinophil count. At the 99th percentile, corresponding to an eosinophil count of 0.75 × 10(9) /l, risks of hematological malignancies were increased more than twofold with OR (95% C.I.) of 2.39 (1.91-2.99). Interestingly, risks reached a plateau around an eosinophil count of 1.0 × 10(9) /l. Risks also increased when the eosinophil count approached zero. Here, counts associated relatively more with acute myeloid leukemia and myelodysplastic syndromes whereas counts above 0.16 × 10(9) /l associated more with myeloproliferative neoplasms. Eosinophil counts associate with hematological malignancies and mortality even below the definition of eosinophilia. The observed plateau of risks around 1.0 × 10(9) /l is important for physicians encountering patients with eosinophilia since even mild-to-moderate eosinophilia according to traditional definitions confers maximally increased risks of subsequent/subclinical hematological malignancy.

  15. Dibutyryl cAMP effects on thromboxane and leukotriene production in decompression-induced lung injury

    Science.gov (United States)

    Little, T. M.; Butler, B. D.

    1997-01-01

    Decompression-induced venous bubble formation has been linked to increased neutrophil counts, endothelial cell injury, release of vasoactive eicosanoids, and increased vascular membrane permeability. These actions may account for inflammatory responses and edema formation. Increasing the intracellular cAMP has been shown to decrease eicosanoid production and edema formation in various models of lung injury. Reduction of decompression-induced inflammatory responses was evaluated in decompressed rats pretreated with saline (controls) or dibutyryl cAMP (DBcAMP, an analog of cAMP). After pretreatment, rats were exposed to either 616 kPa for 120 min or 683 kPa for 60 min. The observed increases in extravascular lung water ratios (pulmonary edema), bronchoalveolar lavage, and pleural protein in the saline control group (683 kPa) were not evident with DBcAMP treatment. DBcAMP pretreatment effects were also seen with the white blood cell counts and the percent of neutrophils in the bronchoalveolar lavage. Urinary levels of thromboxane B2, 11-dehydrothromboxane B2, and leukotriene E4 were significantly increased with the 683 kPa saline control decompression exposure. DBcAMP reduced the decompression-induced leukotriene E4 production in the urine. Plasma levels of thromboxane B2, 11-dehydrothromboxane B2, and leukotriene E4 were increased with the 683-kPa exposure groups. DBcAMP treatment did not affect these changes. The 11-dehydrothromboxane B2 and leukotriene E4 levels in the bronchoalveolar lavage were increased with the 683 kPa exposure and were reduced with the DBcAMP treatment. Our results indicate that DBcAMP has the capability to reduce eicosanoid production and limit membrane permeability and subsequent edema formation in rats experiencing decompression sickness.

  16. High white blood cell count at diagnosis of childhood acute lymphoblastic leukaemia

    DEFF Research Database (Denmark)

    Vaitkeviciene, Goda; Forestier, Erik; Hellebostad, Marit;

    2011-01-01

    Prognostic impact of peripheral blood white blood cell count (WBC) at the diagnosis of childhood acute lymphoblastic leukaemia (ALL) was evaluated in a population-based consecutive series of 2666 children aged 1-15 treated for ALL between 1992 and 2008 in the five Nordic countries (Denmark, Finland...

  17. Sample to answer visualization pipeline for low-cost point-of-care blood cell counting

    Science.gov (United States)

    Smith, Suzanne; Naidoo, Thegaran; Davies, Emlyn; Fourie, Louis; Nxumalo, Zandile; Swart, Hein; Marais, Philip; Land, Kevin; Roux, Pieter

    2015-03-01

    We present a visualization pipeline from sample to answer for point-of-care blood cell counting applications. Effective and low-cost point-of-care medical diagnostic tests provide developing countries and rural communities with accessible healthcare solutions [1], and can be particularly beneficial for blood cell count tests, which are often the starting point in the process of diagnosing a patient [2]. The initial focus of this work is on total white and red blood cell counts, using a microfluidic cartridge [3] for sample processing. Analysis of the processed samples has been implemented by means of two main optical visualization systems developed in-house: 1) a fluidic operation analysis system using high speed video data to determine volumes, mixing efficiency and flow rates, and 2) a microscopy analysis system to investigate homogeneity and concentration of blood cells. Fluidic parameters were derived from the optical flow [4] as well as color-based segmentation of the different fluids using a hue-saturation-value (HSV) color space. Cell count estimates were obtained using automated microscopy analysis and were compared to a widely accepted manual method for cell counting using a hemocytometer [5]. The results using the first iteration microfluidic device [3] showed that the most simple - and thus low-cost - approach for microfluidic component implementation was not adequate as compared to techniques based on manual cell counting principles. An improved microfluidic design has been developed to incorporate enhanced mixing and metering components, which together with this work provides the foundation on which to successfully implement automated, rapid and low-cost blood cell counting tests.

  18. Machine Learning Based Single-Frame Super-Resolution Processing for Lensless Blood Cell Counting

    Directory of Open Access Journals (Sweden)

    Xiwei Huang

    2016-11-01

    Full Text Available A lensless blood cell counting system integrating microfluidic channel and a complementary metal oxide semiconductor (CMOS image sensor is a promising technique to miniaturize the conventional optical lens based imaging system for point-of-care testing (POCT. However, such a system has limited resolution, making it imperative to improve resolution from the system-level using super-resolution (SR processing. Yet, how to improve resolution towards better cell detection and recognition with low cost of processing resources and without degrading system throughput is still a challenge. In this article, two machine learning based single-frame SR processing types are proposed and compared for lensless blood cell counting, namely the Extreme Learning Machine based SR (ELMSR and Convolutional Neural Network based SR (CNNSR. Moreover, lensless blood cell counting prototypes using commercial CMOS image sensors and custom designed backside-illuminated CMOS image sensors are demonstrated with ELMSR and CNNSR. When one captured low-resolution lensless cell image is input, an improved high-resolution cell image will be output. The experimental results show that the cell resolution is improved by 4×, and CNNSR has 9.5% improvement over the ELMSR on resolution enhancing performance. The cell counting results also match well with a commercial flow cytometer. Such ELMSR and CNNSR therefore have the potential for efficient resolution improvement in lensless blood cell counting systems towards POCT applications.

  19. Normal Reference Value of Red Blood Cell Count of Chinese Presenile Men and Geographical Factors

    Institute of Scientific and Technical Information of China (English)

    HE Jinwei; GE Miao; SU Huimin; LIANG Wei; CHEN Hongfei

    2007-01-01

    This paper aims at providing a scientific basis for unifying the normal reference value standards of red blood cell count of Chinese presenile men. The paper, using microscopical counting method, studies the relationship between the normal reference values of 38,061 samples of red blood cell count ofpresenile men and eight geographical factors in297 units in China. It is found that the correlation of geographical factors and the normal reference value of red blood cell count of presenile men is quite significant (F=303.00, P=0.000). By using the method of stepwise regression analysis,one regression equation is inferred. It is concluded that if geographical data are obtained in a certain area, the normal reference value of red blood cell count of presenile men in this area can be reckoned by using the regression analysis.Furthermore, according to the geographical factors, China can be divided into eight regions: Northeast China Region,North China Region, Shanxi-Shaanxi-Inner Mongolia Region, Middle and Lower Reaches of the Changjiang River Region, Southeast China Region, Northwest China Region, Southwest China Region and Qinghai-Tibet Plateau Region.

  20. Children's white blood cell counts in relation to developmental exposures to methylmercury and persistent organic pollutants

    DEFF Research Database (Denmark)

    Oulhote, Y; Shamim, Z; Kielsen, K;

    2017-01-01

    BACKGROUND: To explore possible markers of developmental immunotoxicity, we prospectively examined 56 children to determine associations between exposures to methylmercury and persistent organic pollutants since birth and the comprehensive differential counts of white blood cells (WBC) at age 5......), and total mercury (Hg) were measured in maternal (n=56) and children's blood at 18 months (n=42) and 5 years (n=54). We constructed latent functions for exposures at three different ages using factor analyses and applied structural equation models adjusted for covariates. RESULTS: Prenatal mercury exposure......-cell mediated immunity. CONCLUSION: Developmental exposure to environmental immunotoxicants appears to have different impacts on WBC counts in childhood....

  1. Blood cell counting and classification by nonflowing laser light scattering method

    Science.gov (United States)

    Yang, Ye; Zhang, Zhenxi; Yang, Xinhui; Jiang, Dazong; Yeo, Joon Hock

    1999-11-01

    A new non-flowing laser light scattering method for counting and classifying blood cells is presented. A linear charge- coupled device with 1024 elements is used to detect the scattered light intensity distribution of the blood cells. A pinhole plate is combined with the CCD to compete the focusing of the measurement system. An isotropic sphere is used to simulate the blood cell. Mie theory is used to describe the scattering of blood cells. In order to inverse the size distribution of blood cells from their scattered light intensity distribution, Powell method combined with precision punishment method is used as a dependent model method for measurement red blood cells and blood plates. Non-negative constraint least square method combined with Powell method and precision punishment method is used as an independent model for measuring white blood cells. The size distributions of white blood cells and red blood cells, and the mean diameter of red blood cells are measured by this method. White blood cells can be divided into three classes: lymphocytes, middle-sized cells and neutrocytes according to their sizes. And the number of blood cells in unit volume can also be measured by the linear dependence of blood cells concentration on scattered light intensity.

  2. Evaluation of different sized blood sampling tubes for thromboelastometry, platelet function, and platelet count

    DEFF Research Database (Denmark)

    Andreasen, Jo Bønding; Pistor-Riebold, Thea Unger; Knudsen, Ingrid Hell;

    2014-01-01

    count remained stable using a 3.6 mL tube during the entire observation period of 120 min (p=0.74), but decreased significantly after 60 min when using tubes smaller than 3.6 mL (pblood sampling tubes. Therefore, 1.8 mL tubes should...... be preferred for RoTEM® analyses in order to minimise the volume of blood drawn. With regard to platelet aggregation analysed by impedance aggregometry tubes of different size cannot be used interchangeably. If platelet count is determined later than 10 min after blood sampling using tubes containing citrate......Background: To minimise the volume of blood used for diagnostic procedures, especially in children, we investigated whether the size of sample tubes affected whole blood coagulation analyses. Methods: We included 20 healthy individuals for rotational thromboelastometry (RoTEM®) analyses...

  3. Telomere Length in Elderly Caucasians Weakly Correlates with Blood Cell Counts

    OpenAIRE

    Ewa Gutmajster; Joanna Witecka; Magdalena Wyskida; Justyna Koscinska-Marczewska; Malgorzata Szwed; Magdalena Owczarz; Malgorzata Mossakowska; Andrzej Milewicz; Monika Puzianowska-Kuznicka; Jan Zejda; Andrzej Wiecek; Jerzy Chudek; Aleksander L. Sieron

    2013-01-01

    Background. Age-related decrease in bone marrow erythropoietic capacity is often accompanied by the telomere length shortening in peripheral white blood cells. However, limited and conflicting data hamper the conclusive opinion regarding this relationship. Therefore, the aim of this study was to assess an association between telomere length and peripheral blood cell count parameters in the Polish elderly population. Material and Methods. The substudy included 1573 of 4981 subjects aged 65 yea...

  4. Signatures of reproductive events on blood counts and biomarkers of inflammation: Implications for chronic disease risk

    Science.gov (United States)

    2017-01-01

    Whether inflammation mediates how reproductive events affect chronic-disease risk is unclear. We studied inflammatory biomarkers in the context of reproductive events using National Health and Nutrition Examination Survey (NHANES) data. From 15,986 eligible women from the 1999–2011 data cycles, we accessed information on reproductive events, blood counts, C-reactive protein (CRP), and total homocysteine (tHCY). We calculated blood-count ratios including: platelet-lymphocyte (PLR), lymphocyte-monocyte (LMR), platelet-monocyte (PMR), and neutrophil-monocyte (NMR). Using sampling weights per NHANES guidelines, means for counts, ratios, or biomarkers by reproductive events were compared using linear regression. We performed trend tests and calculated p-values with partial sum of squares F-tests. Higher PLR and lower LMR were associated with nulliparity. In postmenopausal women, lower PMR was associated with early age at first birth and higher NMR with later age at and shorter interval since last birth. Lower PNR and higher neutrophils and tHCY were associated with early natural menopause. In all women, the neutrophil count correlated positively with CRP; but, in premenopausal women, correlated inversely with tHCY. Reproductive events leave residual signatures on blood counts and inflammatory biomarkers that could underlie their links to chronic disease risk. PMID:28234958

  5. A rapid method for counting nucleated erythrocytes on stained blood smears by digital image analysis

    Science.gov (United States)

    Gering, E.; Atkinson, C.T.

    2004-01-01

    Measures of parasitemia by intraerythrocytic hematozoan parasites are normally expressed as the number of infected erythrocytes per n erythrocytes and are notoriously tedious and time consuming to measure. We describe a protocol for generating rapid counts of nucleated erythrocytes from digital micrographs of thin blood smears that can be used to estimate intensity of hematozoan infections in nonmammalian vertebrate hosts. This method takes advantage of the bold contrast and relatively uniform size and morphology of erythrocyte nuclei on Giemsa-stained blood smears and uses ImageJ, a java-based image analysis program developed at the U.S. National Institutes of Health and available on the internet, to recognize and count these nuclei. This technique makes feasible rapid and accurate counts of total erythrocytes in large numbers of microscope fields, which can be used in the calculation of peripheral parasitemias in low-intensity infections.

  6. Periprosthetic joint infection diagnosis: a complete understanding of white blood cell count and differential.

    Science.gov (United States)

    Zmistowski, Benjamin; Restrepo, Camilo; Huang, Ronald; Hozack, William J; Parvizi, Javad

    2012-10-01

    Recent research has raised doubts regarding the utility of serum white blood cell count (WBC) for diagnosis of periprosthetic joint infection (PJI). As synovial WBC and neutrophil (PMN) percentage have been adopted as accurate markers of PJI, this study investigated the correlation of WBC in serum versus joint fluid and diagnostic value of all WBC levels for failed arthroplasty patients. 153 patients (73 PJI) undergoing revision knee arthroplasty were identified. Weak correlations between joint fluid and serum for WBC (R = 0.19), PMN count (R = 0.31), and lymphocyte count (R = -0.22) were observed. Diagnostic accuracy of PMN (93%) and WBC (93%) synovial count relative to serum was similar to synovial WBC (93%) and PMN% (95%) alone. Serum WBC analysis does little to improve the accurate diagnosis of PJI.

  7. Impact of valproates on haemostasis and blood cell count in children

    Directory of Open Access Journals (Sweden)

    Igrutinović Zoran

    2008-01-01

    Full Text Available INTRODUCTION Epilepsy is a highly prevalent disease affecting 0.5-1.5% of the world's population. One of the most frequently used antiepileptics are valproates. These medicines show a negative impact on haemostasis and peripheral blood count. OBJECTIVE The objective of the study was to examine the negative impact of valproates on haemostasis and peripheral blood count in children and to analyse whether these disturbances were dependent on the dosage of valproates and drug level in blood. METHOD A two-year research was conducted. The research included: 35 children using valproates, 12 children using the therapy of both valproates and carbamazepine and 30 healthy children. Complete peripheral blood count, screening tests of haemostasis (bleeding time, prothrombin time, prothrombin ratio, activated partial thromboplastin time, fibrinogen and capacity of thrombocyte aggregation research were done in all the children. RESULTS We found significantly more common frequency of leukopenia and neutropenia in children using valproates in comparison with the healthy children group. We also found the more common frequency of eosinophilia in comparison with healthy children. The children with the valproate therapy have lower approximate values of the number of platelets, fibrinogen and platelet aggregation in comparison with healthy children, but they have a higher approximate value of bleeding time and prothrombin time. These disturbances are in correlation with the dosage and the level of the medicine in blood. CONCLUSION Valproates have a negative effect on certain blood count parameters and haemostasis in children. Drug dosage and blood drug level are correlated with their negative impact on haemostasis parameters.

  8. Complete blood cell count in psittaciformes by using high-throughput image cytometry: a pilot study.

    Science.gov (United States)

    Beaufrère, Hugues; Ammersbach, Mélanie; Tully, Thomas N

    2013-09-01

    The avian hemogram is usually performed in veterinary diagnostic laboratories by using manual cell counting techniques and differential counts determined by light microscopy. There is no standard automated technique for avian blood cell count and differentiation to date. These shortcomings in birds are primarily because erythrocytes and thrombocytes are nucleated, which precludes the use of automated analyzers programmed to perform mammal complete blood cell counts. In addition, there is no standard avian antibody panel, which would allow cell differentiation by immunophenotyping across all commonly seen bird species. We report an alternative hematologic approach for quantification and differentiation of avian blood cells by using high-throughput image cytometry on blood smears in psittacine bird species. A pilot study was designed with 70 blood smears of different psittacine bird species stained with a Wright-Giemsa stain. The slides were scanned at 0.23 microm/pixel. The open-source softwares CellProfiler and CellProfiler Analyst were used for analyzing and sorting each cell by image cytometry. A "pipeline" was constructed in the CellProfiler by using different modules to identify and export hundreds of measures per cell for shape, intensity, and texture. Rules for classifying the different blood cell phenotypes were then determined based on these measurements by iterative feedback and machine learning by using CellProfiler Analyst. Although this approach shows promises, avian Leukopet results could not be duplicated when using this technique as is. Further studies and more standardized prospective investigations may be needed to refine the "pipeline" strategy and the machine learning algorithm.

  9. Concomitant spuriously elevated white blood cell count, a previously underestimated phenomenon in EDTA-dependent pseudothrombocytopenia.

    Science.gov (United States)

    Xiao, Yufei; Xu, Yang

    2015-01-01

    The proportion and potential risk of concomitant spuriously elevated white blood cell count (SEWC) are underestimated in ethylenediaminetetraacetic acid (EDTA)-dependent pseudothrombocytopenia (PTCP). The proportion, kinetics and prevention of SEWC remain poorly understood. A total of 25 patients with EDTA-dependent PTCP were enrolled in this study. With the hematology analyzer Coulter LH 750, we determined the time courses of WBC count, WBC differential and platelet count in EDTA- and sodium citrate-anticoagulated blood, respectively. Blood smears were prepared to inspect the presence of platelet clumps using light microscopy. The effect of automatic instrumental correction on the extent of SEWC was evaluated. The proportion of SEWC was 92% in EDTA-dependent PTCP and 73.9% of SEWCs were within the normal range. The development of SEWC was time-dependent, and neutrophils and lymphocytes were the main subpopulations involved in SEWC. A strong and significant correlation (r = 0.9937, p EDTA were significantly higher than their basal counts, respectively, p 0.05. A high proportion of concomitant SEWCs, which are mainly within normal range, are present in patients with EDTA-dependent PTCP. Proper interpretation of SEWC is crucial to avoid clinic errors. SEWC develops in a time-dependent pattern, although the Coulter LH 750 only partly mitigates the extent of SEWC, sodium citrate is able to effectively prevent SEWC.

  10. Joint Effect of Cigarette Smoking and Body Mass Index on White Blood Cell Count in Korean Adults

    Science.gov (United States)

    Cho, A-Ra; Choi, Won-Jun; Kim, Shin-Hye; Shim, Jae-Yong

    2017-01-01

    Background White blood cell count is an independent risk factor for cardiovascular disease. Several lifestyle and metabolic factors such as cigarette smoking and obesity are known to be associated with an elevated white blood cell count. However, the joint effect of cigarette smoking and obesity on white blood cell count has not yet been fully described. Methods We explored the joint effect of cigarette smoking and obesity on white blood cell count using multiple logistic regression analyses after adjusting for confounding variables in a population-based, cross-sectional study of 416,065 Korean adults. Results Cigarette smoking and body mass index have a dose-response relationship with a higher white blood cell count, but no synergistic interaction is observed between them (men, P for interaction=0.797; women, P for interaction=0.311). Cigarette smoking and body mass index might have an additive combination effect on high white blood cell count. Obese male smokers were 2.36 times more likely and obese female smokers 2.35 times more likely to have a high white blood cell count when compared with normal body mass index non-smokers. Conclusion Cigarette smoking and body mass index are independently associated with an elevated white blood cell count in both men and women. PMID:28360982

  11. A whole blood model of thrombocytopenia that controls platelet count and hematocrit.

    Science.gov (United States)

    Bercovitz, R S; Brenner, M K; Newman, D K

    2016-10-01

    In patients with thrombocytopenia, it can be difficult to predict a patient's bleeding risk based on platelet count alone. Platelet reactivity may provide additional information; however, current clinical assays cannot reliably assess platelet function in the setting of thrombocytopenia. New methods to study platelet reactivity in thrombocytopenic samples are needed. In this study, we sought to develop a laboratory model of thrombocytopenia using blood from healthy subjects that preserves the whole blood environment and reproducibly produces samples with a specific platelet count and hematocrit. We compared the activation state of unstimulated and agonist-stimulated platelets in thrombocytopenic samples derived from this method with normocytic controls. Whole blood was diluted with autologous red blood cell concentrate and platelet-poor plasma, which were obtained via centrifugation, in specific ratios to attain a final sample with a predetermined platelet count and hematocrit. P-selectin exposure and GPIIbIIIa activation in unstimulated platelets and platelets stimulated with collagen-related peptide (CRP) or adenosine diphosphate (ADP) in thrombocytopenic samples and the normocytic control from which they were derived were quantified by flow cytometry. Our methodology reliably produced thrombocytopenic samples with a platelet count ≤50,000/μL and an accurately and precisely controlled hematocrit. P-selectin exposure and GPIIbIIIa activation on unstimulated platelets or on ADP- or CRP-stimulated platelets did not differ in thrombocytopenic samples compared to normocytic controls. We describe a new method for creating thrombocytopenic blood that can be used to better understand the contributions of platelet number and function to hemostasis.

  12. Trajectory of platelets in pregnancy - do low-risk women need an intrapartum full blood count prior to epidural?

    Science.gov (United States)

    Duong, Christine; Kidson-Gerber, Giselle; Peters, Nancy; Listijono, Dave R; Henry, Amanda

    2015-10-01

    This study aimed to investigate whether pregnant women with a normal 28-week gestation platelet count and no high-risk conditions for thrombocytopenia require a pre-epidural platelet count. All 1844 included women (platelet count > 150 × 10(9) /L at 28 weeks' gestation, term singleton birth, no thrombocytopenia risk conditions) had a platelet count > 100 × 10(9) /L prebirth, suggesting low-risk pregnant women do not require pre-epidural full blood count solely to check platelet count.

  13. In situ microdialysis of intramuscular prostaglandin and thromboxane in contracting skeletal muscle in humans

    DEFF Research Database (Denmark)

    Karamouzis, M; Langberg, Henning; Skovgaard, D;

    2001-01-01

    Arachidonic acid metabolites, especially prostacyclin I2, are regulators of vascular tone, and may be released from contracting muscle. In the present study, the influence of exercise on accumulation of prostaglandins and thromboxane in skeletal muscle was determined by the use of microdialysis...... amounts of prostaglandins and thromboxanes in the interstitial space of skeletal muscle. Furthermore, the concentration of prostaglandin E2 is unchanged during static calf exercise and increased markedly with dynamic thigh muscle exercise, which together with an exercise induced increase in muscle blood...... flow indicate, that prostaglandin E2 is released from skeletal muscle during exercise in humans....

  14. HEMODOSE: A Biodosimetry Tool Based on Multi-type Blood Cell Counts.

    Science.gov (United States)

    Hu, Shaowen; Blakely, William F; Cucinotta, Francis A

    2015-07-01

    Peripheral blood cell counts are important biomarkers of radiation exposure. In this work, a simplified compartmental modeling approach is applied to simulate the perturbation of the hematopoiesis system in humans after radiation exposure, and HemoDose software is reported to estimate individuals' absorbed doses based on multi-type blood cell counts. Testing with patient data in some historical accidents indicates that either single or serial granulocyte, lymphocyte, leukocyte, and platelet counts after exposure can be robust indicators of the absorbed doses. In addition, such correlation exists not only in the early time window (1 or 2 d) but also in the late phase (up to 4 wk) after exposure, when the four types of cell counts are combined for analysis. These demonstrate the capability of HemoDose as a rapid point-of-care diagnostic or centralized high-throughput assay system for personnel exposed to unintended high doses of radiation, especially in large-scale nuclear/radiological disaster scenarios involving mass casualties.

  15. The Influence of Low Platelet Count on Whole Blood Aggregometry Assessed by Multiplate

    DEFF Research Database (Denmark)

    Stissing, Trine; Dridi, Nadia P; Ostrowski, Sisse R;

    2011-01-01

    The Multiplate, a whole blood (WB) platelet function test, has shown promising results identifying patients on antiplatelet therapy at increased risk of rethrombosis. In the present study, the influence of low platelet count on platelet aggregation was analyzed and compared with aggregation results...... in an artificial matrix, platelet-rich plasma (PRP). Heparinized and citrated blood was diluted with autologous plasma to platelet concentrations 200 to 25 × 10(9)/L in WB samples (n = 10) and 200 to 100 × 10(9)/L in PRP samples (n = 7). The platelet aggregation was investigated by the ADP-, ASPI-, COL-, and TRAP...

  16. Neutropenia Prediction Based on First-Cycle Blood Counts Using a FOS-3NN Classifier

    Directory of Open Access Journals (Sweden)

    Elize A. Shirdel

    2011-01-01

    Full Text Available Background. Delivery of full doses of adjuvant chemotherapy on schedule is key to optimal breast cancer outcomes. Neutropenia is a serious complication of chemotherapy and a common barrier to this goal, leading to dose reductions or delays in treatment. While past research has observed correlations between complete blood count data and neutropenic events, a reliable method of classifying breast cancer patients into low- and high-risk groups remains elusive. Patients and Methods. Thirty-five patients receiving adjuvant chemotherapy for early-stage breast cancer under the care of a single oncologist are examined in this study. FOS-3NN stratifies patient risk based on complete blood count data after the first cycle of treatment. All classifications are independent of breast cancer subtype and clinical markers, with risk level determined by the kinetics of patient blood count response to the first cycle of treatment. Results. In an independent test set of patients unseen by FOS-3NN, 19 out of 21 patients were correctly classified (Fisher’s exact test probability P<0.00023 [2 tailed], Matthews’ correlation coefficient +0.83. Conclusions. We have developed a model that accurately predicts neutropenic events in a population treated with adjuvant chemotherapy in the first cycle of a 6-cycle treatment.

  17. Estimation of platelet count in unstained peripheral blood smears in comparison with stained smears and evaluation of its efficacy.

    Science.gov (United States)

    Umashankar, T; Thomas, B M; Sahana, P

    2014-12-01

    Assessment of platelet count is an important diagnostic parameter in haematology. Automated blood cell counters have largely replaced the manual method. However, all abnormal platelet counts are verified in Leishman's stained peripheral blood smear. Platelets also can be identified in the unstained blood smears. The objective of this study is to compare the unstained peripheral blood smears with the stained smears and determination of the effectiveness of unstained smears in the estimation of platelet count. 250 Venous blood samples sent for blood cell counts were analyzed. Platelets were counted in the unstained smear under 100× objective in 10 fields without placing immersion oil and the average number of platelets was calculated. Same smear was stained by Leishman's stain and platelets were counted under 100× objective after placing a drop of immersion oil. Collected data were analyzed for intra-class correlation coefficient (ICC). ICC showed excellent agreement (ICC > 0.85). The unstained smears were found to be as effective as stained smears for platelet count in most of the cases. However, in case of doubt a stained preparation has to be made to confirm the count. The turnaround time was 3-5 minutes compared to 15-20 minutes by stained smear technique, thus this technique may be used as an initial screening method whenever there is large sample load.

  18. Telomere Length in Elderly Caucasians Weakly Correlates with Blood Cell Counts

    Directory of Open Access Journals (Sweden)

    Ewa Gutmajster

    2013-01-01

    Full Text Available Background. Age-related decrease in bone marrow erythropoietic capacity is often accompanied by the telomere length shortening in peripheral white blood cells. However, limited and conflicting data hamper the conclusive opinion regarding this relationship. Therefore, the aim of this study was to assess an association between telomere length and peripheral blood cell count parameters in the Polish elderly population. Material and Methods. The substudy included 1573 of 4981 subjects aged 65 years or over, participants of the population-based PolSenior study. High-molecular-weight DNA was isolated from blood mononuclear cells. Telomere length (TL was measured by QRT-PCR as abundance of telomere template versus a single gene copy encoding acidic ribosomal phosphoprotein P0. Results. Only white blood count (WBC was significantly different in TL tertile subgroups in all subjects (P=0.02 and in men (P=0.01, but not in women. Merely in men significant but weak positive correlations were found between TL and WBC (r=0.11, P<0.05 and RBC (r=0.08, P<0.05. The multiple regression analysis models confirmed a weak, independent contribution of TL to both RBC and WBC. Conclusions. In the elderly, telomere shortening limits hematopoiesis capacity to a very limited extent.

  19. Amikacin can be added to blood to reduce the fall in platelet count.

    Science.gov (United States)

    Zhou, Xiaomian; Wu, Xiaoli; Deng, Weixiong; Li, Jieqiu; Luo, Wenshen

    2011-10-01

    Our objective was to develop an effective method to prevent the fall in platelet count for patients with anticoagulant-dependent (AD) pseudothrombocytopenia, a spurious phenomenon due to anticoagulant-induced aggregation of platelets. We report a case of insidious multianticoagulant-dependent pseudothrombocytopenia in which AD pseudothrombocytopenia may be caused by 4 anticoagulants, eg, EDTA, sodium citrate, heparin, and sodium fluoride (NaF). Multianticoagulant-dependent pseudothrombocytopenia was confirmed by finding clumped platelets on microscopic evaluation in 4 anticoagulated blood samples. With this case, we tried a variety of reagents, including aminoglycosides, eg, gentamicin and amikacin, vitamin B(6), and aminophylline to inhibit pseudothrombocytopenia. Except for amikacin, all reagents failed to prevent pseudothrombocytopenia. Microscopic examination of K(2)-EDTA-, heparin-, sodium citrate-, and NaF-anticoagulated blood samples showed massive platelet clumping, but no aggregate was seen in the anticoagulated blood with amikacin. When amikacin was added within 1 hour after blood sample withdrawal, platelet, WBC, and RBC counts and hemoglobin level, mean corpuscular volume, and mean platelet volume remained unchanged for up to 4 hours at room temperature. These findings suggest that amikacin could inhibit and dissociate pseudo platelet aggregation in multianticoagulant-dependent pseudothrombocytopenia and EDTA-induced pseudothrombocytopenia.

  20. Telomere Length in Elderly Caucasians Weakly Correlates with Blood Cell Counts

    Science.gov (United States)

    Witecka, Joanna; Koscinska-Marczewska, Justyna; Szwed, Malgorzata; Owczarz, Magdalena; Mossakowska, Malgorzata; Milewicz, Andrzej; Zejda, Jan; Wiecek, Andrzej

    2013-01-01

    Background. Age-related decrease in bone marrow erythropoietic capacity is often accompanied by the telomere length shortening in peripheral white blood cells. However, limited and conflicting data hamper the conclusive opinion regarding this relationship. Therefore, the aim of this study was to assess an association between telomere length and peripheral blood cell count parameters in the Polish elderly population. Material and Methods. The substudy included 1573 of 4981 subjects aged 65 years or over, participants of the population-based PolSenior study. High-molecular-weight DNA was isolated from blood mononuclear cells. Telomere length (TL) was measured by QRT-PCR as abundance of telomere template versus a single gene copy encoding acidic ribosomal phosphoprotein P0. Results. Only white blood count (WBC) was significantly different in TL tertile subgroups in all subjects (P = 0.02) and in men (P = 0.01), but not in women. Merely in men significant but weak positive correlations were found between TL and WBC (r = 0.11, P < 0.05) and RBC (r = 0.08, P < 0.05). The multiple regression analysis models confirmed a weak, independent contribution of TL to both RBC and WBC. Conclusions. In the elderly, telomere shortening limits hematopoiesis capacity to a very limited extent. PMID:24453794

  1. Preoperative White Blood Cell Count and Risk of 30-Day Readmission after Cardiac Surgery

    Directory of Open Access Journals (Sweden)

    Jeremiah R. Brown

    2013-01-01

    Full Text Available Approximately 1 in 5 patients undergoing cardiac surgery are readmitted within 30 days of discharge. Among the primary causes of readmission are infection and disease states susceptible to the inflammatory cascade, such as diabetes, chronic obstructive pulmonary disease, and gastrointestinal complications. Currently, it is not known if a patient’s baseline inflammatory state measured by crude white blood cell (WBC counts could predict 30-day readmission. We collected data from 2,176 consecutive patients who underwent cardiac surgery at seven hospitals. Patient readmission data was abstracted from each hospital. The independent association with preoperative WBC count was determined using logistic regression. There were 259 patients readmitted within 30 days, with a median time of readmission of 9 days (IQR 4–16. Patients with elevated WBC count at baseline (10,000–12,000 and >12,000 mm3 had higher 30-day readmission than those with lower levels of WBC count prior to surgery (15% and 18% compared to 10%–12%, P=0.037. Adjusted odds ratios were 1.42 (0.86, 2.34 for WBC counts 10,000–12,000 and 1.81 (1.03, 3.17 for WBC count > 12,000. We conclude that WBC count measured prior to cardiac surgery as a measure of the patient’s inflammatory state could aid clinicians and continuity of care management teams in identifying patients at heightened risk of 30-day readmission after discharge from cardiac surgery.

  2. On-chip sample preparation for complete blood count from raw blood.

    Science.gov (United States)

    Nguyen, John; Wei, Yuan; Zheng, Yi; Wang, Chen; Sun, Yu

    2015-03-21

    This paper describes a monolithic microfluidic device capable of on-chip sample preparation for both RBC and WBC measurements from whole blood. For the first time, on-chip sample processing (e.g. dilution, lysis, and filtration) and downstream single cell measurement were fully integrated to enable sample preparation and single cell analysis from whole blood on a single device. The device consists of two parallel sub-systems that perform sample processing and electrical measurements for measuring RBC and WBC parameters. The system provides a modular environment capable of handling solutions of various viscosities by adjusting the length of channels and precisely controlling mixing ratios, and features a new 'offset' filter configuration for increased duration of device operation. RBC concentration, mean corpuscular volume (MCV), cell distribution width, WBC concentration and differential are determined by electrical impedance measurement. Experimental characterization of over 100,000 cells from 10 patient blood samples validated the system's capability for performing on-chip raw blood processing and measurement.

  3. Changes in complete blood count in patients with surgically treated facial fractures.

    Science.gov (United States)

    Milani, Thiago Augusto Picosse; Rodrigues, Lucimar; Chiattone, Carlos; Luz, João Gualberto C

    2012-11-01

    The purpose of this prospective study was to verify the changes in the preoperative and postoperative complete blood counts of patients with surgically treated facial fractures. Fifty consecutive patients with a mean age of 34 years who presented facial fractures and underwent surgical treatment were included. A complete blood count was performed, comprising the red and white blood cell count (cells/μL), hemoglobin (g/dL), and hematocrit (%) levels. These data were obtained preoperatively and postoperatively during a 6-week period. Statistical analyses were performed using the Kruskal-Wallis and Mann-Whitney tests to identify the possible differences among the groups and among the periods of observation using the Friedman and Wilcoxon matched-pairs signed-ranks tests. The most common location of the fractures was the mandible (42.3%), followed by the zygomatic-orbital (36.5%) and associated locations (21.2%). Leukocytosis was associated with neutrophilia in the immediate postoperative period in all of the groups. There were no values below the reference limits of the values of hemoglobin, hematocrit, and erythrocytes, and no values above the reference limits for the remaining white blood cells, although significant differences among periods were observed in most cells, depending on the type of fracture. The primary findings were leukocytosis associated with neutrophilia, verified in the immediate postoperative period in all of the groups, and the influence of the type of fracture on the significant alterations observed among studied periods on the values of hemoglobin, hematocrit, erythrocytes, leukocytes, neutrophils, and lymphocytes.

  4. Sex differences in step count-blood pressure association: a preliminary study in type 2 diabetes.

    Directory of Open Access Journals (Sweden)

    Priya Manjoo

    Full Text Available BACKGROUND: Walking and cardiovascular mortality are inversely associated in type 2 diabetes, but few studies have objectively measured associations of walking with individual cardiovascular risk factors. Such information would be useful for "dosing" daily steps in clinical practice. This study aimed to quantify decrements in blood pressure and glycated hemoglobin (A1C per 1,000 daily step increments. METHODOLOGY/PRINCIPAL FINDINGS: Two hundred and one subjects with type 2 diabetes underwent assessments of step counts (pedometer-measured, blood pressure, A1C and anthropometric parameters. Due to missing data, the final analysis was conducted on 83 women and 102 men, with a mean age of 60 years. Associations of daily steps with blood pressure and A1C were evaluated using sex-specific multivariate linear regression models (adjusted for age, ethnicity, and BMI. Potential sex differences were confirmed in a combined model (women and men with interaction terms. Mean values for daily steps, blood pressure, A1C and BMI were 5,357 steps/day; 137/80 mm Hg; 7.7% and 30.4 kg/m(2 respectively. A 1,000 daily step increment among women was associated with a -2.6 (95% CI: -4.1 to -1.1 mm Hg change in systolic and a -1.4 (95% CI: -2.2 to -0.6 mm Hg change in diastolic blood pressure. Among men, corresponding changes were -0.7 (95% CI: -2.1 to 0.7 and -0.6 (95% CI: -1.4 to 0.3 mm Hg, respectively. Sex differences were confirmed in combined models. Step counts and A1C did not demonstrate clinically important associations. CONCLUSIONS/SIGNIFICANCE: A 1,000 steps/day increment is associated with important blood pressure decrements among women with type 2 diabetes but the data were inconclusive among men. Targeted "dose increments" of 1,000 steps/day in women may lead to measurable blood pressure reductions. This information may be of potential use in the titration or "dosing" of daily steps. No associations were found between step count increments and A1C.

  5. THE EFFECT OF BLOOD AND MILK SERUM ZINC CONCENTRATION ON MILK SOMATIC CELL COUNT IN DAIRY COWS

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    Ivana Davidov

    2016-11-01

    Full Text Available The objective of this study was to evaluate the effect of blood and milk zinc concentration on somatic cell count and occurrence of subclinical mastitis cases. The study was performed on thirty Holstein cows approximate same body weight, ages 3 to 5 years, with equally milk production. Blood samples were taken after the morning milking from the caudal vein and milk from all four quarters was taken before morning milking. All samples of blood and milk were taken to determined zinc, using inductively coupled plasma mass spectrometry. 37.67% (11/30 cows have blood serum zinc concentration below 7µmol/l, and 63.33% or 19/30 cows have blood serum zinc concentration higher then 13µmol/l. Also 30% (9/30 cows have somatic cell count lower then 400.000/ml which indicate absence of subclinical mastitis, but 70% (21/30 cows have somatic cell count higher then 400.000/ml which indicate subclinical mastitis. Results indicate that cows with level of zinc in blood serum higher then 13 µmol/l have lower somatic cell count. Cows with lower zinc blood serum concentration then 7 µmol/l have high somatic cell count and high incidence of subclinical mastitis. According to results in this research there is no significant effect of milk serum zinc concentration on somatic cell count in dairy cows.

  6. Complete blood count using VCS (volume, conductivity, light scatter) technology is affected by hyperlipidemia in a child with acute leukemia.

    Science.gov (United States)

    Gokcebay, D G; Azik, F M; Isik, P; Bozkaya, I O; Kara, A; Tavil, E B; Yarali, N; Tunc, B

    2011-12-01

    Asparaginase, an effective drug in the treatment of childhood acute lymphoblastic leukemia (ALL), has become an important component of most childhood ALL regimens during the remission induction or intensification phases of treatment. The incidence range of asparaginase-associated lipid abnormalities that are seen in children is 67-72%. Lipemia causes erroneous results, which uses photometric methods to analyze blood samples. We describe a case of l-asparaginase-associated severe hyperlipidemia with complete blood count abnormalities. Complete blood count analysis was performed with Beckman COULTER(®) GEN·S™ system, which uses the Coulter Volume, Conductivity, Scatter technology to probe hydrodynamically focused cells. Although an expected significant inaccuracy in hemoglobin determination occurred starting from a lipid value of 3450 mg/dl, we observed that triglyceride level was 1466 mg/dl. Complete blood count analysis revealed that exceptionally high hemoglobin, mean corpuscular hemoglobin, and mean corpuscular hemoglobin concentration levels vs. discordant with red blood cell count, mean corpuscular volume, and hematocrit levels. Total leukocyte count altered spontaneously in a wide range, and was checked with blood smear. Platelet count was in expected range (Table 1). Thus, we thought it was a laboratory error, and the patient's follow-up especially for red cell parameters was made by red blood cell and hematocrit values.

  7. Hemograma do coelho normal e mixomatoso Blood counting of normal and mixomatous rabbits

    Directory of Open Access Journals (Sweden)

    Alvaro Emery Trindade

    1954-06-01

    Full Text Available O autor procurou estabelecer os dados relativos ao hemograma de coelho normal, do biotério do Instituto Oswaldo Cruz, encontrando as seguintes médias: hematias 5.186.000 por mm3, leucócitos 10.419 por mm3, e na contagem específica os seguintes valores para Heterófilos 40%, basófilos 2.5%, eosinófilos 0.2%, monócitos 6.5% e linfócitos 50%, e células de Tuerk 0.8%. Pode o autor confirmar o achado de AGOSIN, referente à presença de células morfológicamente semelhantes ás de TUERK, no sangue periférico dos coelhos normais. Realizou ainda, uma revisão bibliográfica do assunto.The author has established data on blood counting of normal rabbits, from the Bioterio of Instituto Oswaldo Cruz, having found the following averages: red cells 5,186,000 per cu. milimeter; white blood cells, 10,419 per cu. milimeter. For shilling's counting, the following values: Heterophiles, 40% basophiles, 2,5%; eosinophiles, 0,2%; monocites, 6,5%; lymphocites 50% and Tuerk's cells, 0,8%. The author confirms Agosin's discovery on the presence of cells morphologically similar to Tuerk's in peripheric blood of normal rabbits. Has done, also, a bibliographical research on this subject.

  8. Depressive symptoms and white blood cell count in coronary heart disease patients : Prospective findings from the Heart and Soul Study

    NARCIS (Netherlands)

    Duivis, Hester E.; Kupper, Nina; Penninx, Brenda W.; Na, Beeya; de Jonge, Peter; Whooley, Mary A.

    2013-01-01

    Background: Depression has been associated with elevated white blood cell (WBC) count - indicative of systemic inflammation - in cross-sectional studies, but no longitudinal study has evaluated whether depressive symptoms predict subsequent WBC count or vice versa. We sought to evaluate the bidirect

  9. A Multiple Parameters Biodosimetry Tool with Various Blood Cell Counts - the Hemodose Approach

    Science.gov (United States)

    Hu, Shaowen

    2014-01-01

    There continue to be important concerns about the possibility of the occurrence of acute radiation syndromes following nuclear and radiological terrorism or accidents that may result in mass casualties in densely populated areas. To guide medical personnel in their clinical decisions for effective medical management and treatment of the exposed individuals, biological markers are usually applied to examine radiation induced biological changes to assess the severity of radiation injury to sensitive organ systems. Among these the peripheral blood cell counts are widely used to assess the extent of radiation induced bone marrow injury. This is due to the fact that the hematopoietic system is the most vulnerable part of the human body to radiation damage. Particularly, the lymphocyte, granulocyte, and platelet cells are the most radiosensitive of the blood elements, and monitoring their changes after exposure is regarded as a practical and recommended laboratory test to estimate radiation dose and injury. Based upon years of physiological and pathophysiological investigation of mammalian hematopoietic systems, and rigorous coarse-grained bio-mathematical modeling and validation on species from mouse, to dog, monkey, and human, we have developed a set of software tools Hemodose, which can use single or serial granulocyte, lymphocyte, leukocyte, or platelet counts after exposure to estimate absorbed doses of adult victims very rapidly and accurately. Some patient data from historical accidents are utilized as examples to demonstrate the capabilities of these tools as a rapid point-of-care diagnostic or centralized high-throughput assay system in a large-scale radiological disaster scenario. Most significant to the improvement of national and local preparedness of a potential nuclear/radiological disaster, this HemoDose approach establishes robust correlations between the absorbed doses and victim's various types of blood cell counts not only in the early time window (1

  10. Possibility of myelodysplastic syndromes screening using a complete blood automated cell count.

    Science.gov (United States)

    Rocco, Vincenzo; Maconi, Mariacaterina; Gioia, Maria; Silvestri, Maria Grazia; Tanca, Donatella; Catalano, Teodora; Avino, Daniela; Di Palma, Anna; Rovetti, Adele; Danise, Paolo

    2011-12-01

    Diagnosis of MDS has changed during the last years because of the 2008 WHO classification. Complete blood cell count (CBC) is a very important tool both for diagnosis of MDS. The aim of this study was to evaluate if it is possible to use the abnormal signals produced by dysplastic cells to produce a flag "dysplasia" able to identify the patients needing further hematological investigations. The proposed flag has been tested in a large group of patients to evaluate the sensibility and specificity. We create 5 patterns of MDS. Our study demonstrated that the flag "dysplasia" is specific and sensible for MDS.

  11. Effect of mycophenolate mofetil on the white blood cell count and the frequency of infection in systemic lupus erythematosus.

    Science.gov (United States)

    Subedi, Ananta; Magder, Laurence S; Petri, Michelle

    2015-10-01

    Leukopenia is a common manifestation of SLE. Addition of immunosuppressive therapy in a SLE patient who is already leukopenic is a clinical concern. It could worsen leukopenia, increase the risk of infection, or both. The aim of this study was to analyze the immediate effect of mycophenolate mofetil on the white blood cell count and the rate of infection in SLE patients. Two hundred and forty-four patients within the Hopkins Lupus Cohort who were newly started on mycophenolate mofetil were included in the study. The white blood cell count and interval infection history on the day mycophenolate mofetil was started were compared with the white blood cell count and interval infection history at the next visit. The study was based on 244 patients who began taking mycophenolate mofetil in the cohort. The study population included 47 % African Americans, 44 % Caucasians, and 9 % other ethnicities. There was a slight but not statistically significant increase in the white blood cell count (6.63 vs. 7.01), after starting mycophenolate mofetil. Patients with a baseline white blood cell count blood cell count after starting mycophenolate mofetil (2.57 vs. 5.13, P = 0.0047). We also found a statistically significant increase in the risk of bacterial infection (but not viral infection) after starting mycophenolate mofetil (4 vs. 9 %, P = 0.0036). Leukopenia does not worsen with mycophenolate mofetil. However, mycophenolate mofetil appears to slightly increase the rate of bacterial (but not viral) infection.

  12. The effects of transport by pneumatic tube system on blood cell count, erythrocyte sedimentation and coagulation tests

    OpenAIRE

    Koçak, Fatma Emel; Yöntem, Mustafa; Yücel, Özlem; Çilo, Mustafa; Genç, Özlem; Meral, Ayfer

    2013-01-01

    Introduction: Today, the pneumatic tube transport system (PTS) is used frequently because of its advantages related to timing and speed. However, the impact of various types of PTS on blood com-ponents is unknown. The aim of this study was to examine the influence of PTS on the quality of rou-tine blood cell counts, erythrocyte sedimentation, and certain blood coagulation tests. Materials and methods: Paired blood samples were obtained from each of 45 human volunteers and evaluated by bloo...

  13. Are fibrinogen and complete blood count parameters predictive in incarcerated abdominal hernia repair?

    Science.gov (United States)

    Kahramanca, Sahin; Kaya, Oskay; Ozgehan, Gulay; Guzel, Hakan; Azili, Cem; Gokce, Emre; Kucukpinar, Tevfik; Kulacoglu, Hakan

    2014-01-01

    Therapeutic delays in cases of external incarcerated hernias typically result in increasing morbidity, mortality, and health expenditures. We investigated the diagnostic role of blood fibrinogen level, white blood count (WBC), mean platelet volume (MPV), and platelet distribution width (PDW) in patients with incarcerated hernia. Two groups, each containing 100 patients, were studied. Group A underwent elective, and group B underwent incarcerated and urgent external hernia repair. We observed high fibrinogen and WBC levels but low MPV and PDW values for patients in group B. Contrary to our expectations, we found lower MPV and PDW values in the complicated group than in the elective group. The morbidity rate and cost burden were higher in group B, and the results were statistically significant. Early operation should be recommended for patients with incarcerated external hernias if their fibrinogen and WBC levels are high.

  14. Large Scale-Invariant Fluctuations in Normal Blood Cell Counts A sign of criticality?

    CERN Document Server

    Perazzo, C A; Chialvo, D R; Willshaw, P; Perazzo, Carlos A.; Fernandez, Elmer A.; Chialvo, Dante R.; Willshaw, Peter

    2000-01-01

    All types of blood cells are formed by differentiation from a small self-maintaining population of pluri-potential stem cells in the bone marrow. Despite abundant information on the molecular aspects of division, differentiation, commitment and maturation of these cells, comparatively little is known about the dynamics of the system as a whole, and how it works to maintain this complex ``ecology'' in the observed normal ranges throughout life. Here we report unexpected large, scale-free, fluctuations detected from the first long-term analysis of the day-to-day variability of a healthy animal's blood cell counts measured over one thousand days. This scale-invariance cannot be accounted for by current theoretical models, and resembles some of the scenarios described for self-organized criticality.

  15. [Clinical characteristics of splenic marginal zone lymphoma with abnormal complete blood count].

    Science.gov (United States)

    Yang, Shen-Miao; Jiang, Qian; Jiang, Bin; Chen, Ding-Bao; Wang, Jing; Jiang, Hao; Lu, Jin; Lu, Xi-Jing; Bao, Li; Shi, Hong-Xia; Liu, Yan-Rong; Huang, Xiao-Jun

    2013-02-01

    The aim of this study was to investigate the clinical and laboratorial characteristics of splenic marginal zone lymphoma (SMZL) with an abnormal complete blood count (CBC). Data of 19 newly diagnosed SMZL patients with abnormal CBC were analyzed retrospectively. Seven patients were diagnosed by using splenic histology, 12 patients who did not undergo splenectomy were diagnosed on the basis of typical clinical presentation and cytologic, immunophenotypic and histologic characteristics of peripheral blood and bone marrow, according to SBLG guidelines. The results showed that leukocytosis (≥ 10.0×10(9)/L) was seen in 5 cases (26.3%); leukocytopenia (complete response was achieved in 9 out of 11 (81.8%) patients. It is concluded that SMZL with abnormal CBC has a higher incidence of cytopenia, bone marrow involvement and autoimmune phenomena. Therapeutic strategies consisting of Rituximab show a better efficacy.

  16. 新风胶囊对膝骨关节炎患者血液流变学指标及血栓素、前列环素的影响%Effects of Xinfeng Capsule on Blood Rheological Indexes,Thromboxane and Prostacyclin of Patients with Knee Osteoarthritis

    Institute of Scientific and Technical Information of China (English)

    陈瑞莲; 刘健; 黄传兵; 王桂珍; 武敏

    2015-01-01

    [ABSTRACT]Objective:To observe the therapeutic effect of Xinfeng Capsule (新风胶囊) in the treatment of knee osteoarthritis,and explore its mechanisms from the aspects of blood rheology,thromboxane and prostacyclin.Methods:80 cases of knee osteoarthritis caused by damp abundance due to splenic deifciency and blood stasis stagnation were randomly divided into a treatment group and a control group,40 cases in each. Another 30 healthy persons were seen as the normal group.The control group were orally given glucosamine sulfate, 314 mg each time and 3 times a day;while the treatment group,based on the treatment of the control group,were given Xinfeng Capsule,1.2 g each time and 3 times a day.Both groups were treated for 2 weeks.Changes of blood rheological indexes(blood viscosity,plasma viscosity,and ifbrinogen),thromboxane(TAX2),prostacyclin(PGI2) and serum inflammatory cytokines (TNF-α,IL-1 and IL-10) were observed before and after treatment. Results:Compared with the normal group,the blood rheological indexes of the treatment group and the control group were abnormal,the content of TAX2,IL-1 and TNF-α increased,and PGI2 (P 0.05). The treatment group was better than the control group in improving blood rheological index,adjusting the balance of TAX2 and PGI2,and balancing serum inlfammatory cytokines (P0.05),在改善血液流变学指标,调节TXA2、PGI2平衡,血清炎性细胞因子平衡方面优于对照组(P<0.05)。结论:膝骨关节炎存在血瘀现象,新风胶囊治疗膝骨关节炎有一定疗效,并可改善血液流变学指标,调节TXA2、PGI2平衡,上调致炎因子,下调抑炎因子,为中医药防治骨关节炎提供理论和实验依据。

  17. Effectiveness using higher inhaled corticosteroid dosage in patients with COPD by different blood eosinophilic counts

    Directory of Open Access Journals (Sweden)

    Cheng SL

    2016-09-01

    Full Text Available Shih-Lung Cheng,1,2 Ching-Hsiung Lin3–5 1Department of Internal Medicine, Far Eastern Memorial Hospital, Taipei, 2Department of Chemical Engineering and Materials Science, Yuan Ze University, Zhongli, Taoyuan City, 3Department of Internal Medicine, Division of Chest Medicine, Changhua Christian Hospital, Changhua, 4Department of Respiratory Care, College of Health Sciences, Chang Jung Christian University, Tainan, 5School of Medicine, Chung Shan Medical University, Taichung City, Taiwan Background: Blood eosinophil counts have been documented as a good biomarker for patients with chronic obstructive pulmonary disease (COPD using inhaled corticosteroid (ICS therapy. However, the effectiveness and safety of prescribing high or medium dose of ICS for patients with different eosinophil counts are unknown.Methods: A post hoc analysis of a previous prospective randomized study was performed for COPD patients using higher dose (HD: Fluticasone 1,000 µg/day or medium dose (MD: Fluticasone 500 µg/day of ICS combined with Salmeterol (100 µg/day. Patients were classified into two groups: those with high eosinophil counts (HE ≥3% and those with low eosinophil counts (LE <3%. Lung function was evaluated with forced expiratory volume in 1 second, forced vital capacity, and COPD assessment test. Frequencies of acute exacerbation and pneumonia were also measured.Results: Two hundred and forty-eight patients were studied and classified into higher eosinophil (HE (n=85, 34.3% and lower eosinophil (LE groups (n=163, 65.7%. The levels of forced expiratory volume in 1 second were significantly increased in patients of HE group treated with HD therapy, compared with the other groups (HE/HD: 125.9±27.2 mL vs HE/MD: 94.3±23.7 mL, vs LE/HD: 70.4±20.5 mL, vs LE/MD: 49.8±16.7 mL; P<0.05 at the end of the study. Quality of life (COPD assessment test markedly improved in HE/HD group than in MD/LE group (HE/HD: 9±5 vs LE/MD: 16±7, P=0.02. The frequency of acute

  18. Dose and dose rate effects of irradiation on blood count and cytokine assay in mice

    Energy Technology Data Exchange (ETDEWEB)

    Kim, Joong Sun [Research center, Dongnam institute of radiological and Medical Sciences (DIRAMS), Busan (Korea, Republic of)

    2013-11-15

    The possible role of exposure to radiation as a risk factor for human health has been of increasing public concern in the series of explosions at earthquake damaged nuclear reactors on the Japan. Current events throughout the world underscore the growing threat of different forms of accidental exposure to radiation including nuclear accidents, atomic weapons use and testing, and the side effects of cancer therapy. A large range of dose rates of ionizing radiations could be encountered in accidental radiation situations. Nevertheless, most of the studies related to radiation effects have only examined a high dose rate. In this study, we investigated the blood count and the cytokine levels in the serum of mice exposed to a high or low dose rate of radiation. In this study, the precise molecular mechanism underlying the low dose rate of radiation remains unclear, but differential hematopoietic effects of radiation exposed at a high dose rate versus low dose rate were observed using the number of peripheral blood count and serum cytokines. These data suggest that chronic low dose rate exposure caused a stimulation of heamatopoietic system occurrence, unlike those observed after higher dose rate exposure. Our data suggest that the dose rate, rather than the total dose, may be more critical in causing damage to the cellular hematopoietic compartments of the body.

  19. The Relationship Between Some Complete Blood Count Parameters and Myocardial Perfusion: A Scintigraphic Approach.

    Science.gov (United States)

    Ozdemir, Semra; Barutcu, Ahmet; Gazi, Emine; Tan, Yusuf Ziya; Turkon, Hakan

    2015-01-01

    Recent studies have shown that there is a relationship between some inflammatory and biochemical markers derived from complete blood count (CBC) such as neutrophil/lymphocyte (N/L) ratio, platelet/lymphocyte (P/L) ratio, platelet distribution width (PDW), red blood cell distribution width (RDW), and coronary artery disease (CAD). The aim of this study was to determine N/L ratio, P/L ratio, PDW values, and RDW values, which are associated with myocardial perfusion in patients diagnosed with CAD. This study included 262 patients (149 with myocardial ischemia/infarction and 113 with normal myocardial perfusion) undergoing myocardial perfusion scintigraphy (MPS) with CBC within 90 days of MPS. Myocardial perfusion parameters such as summed stress score and summed difference score (SDS) were compared with N/L ratio, P/L ratio, PDW values, and RDW values. Neutrophil counts and N/L ratios were significantly higher in patients diagnosed with myocardial ischemia and/or infarct. However, there was no statistically significant relationship between myocardial perfusion abnormalities and P/L ratio, PDW values, and RDW values. This study showed that N/L ratio is related to myocardial ischemia/infarction and correlated to left ventricular ejection fraction (LVEF).

  20. [Automated measurement of reticulocyte count by flow cytometry. II: Analysis of the blood containing abnormal erythrocytes or giant platelets].

    Science.gov (United States)

    Oyamatsu, T; Shimizu, N; Takeuchi, K; Yamamoto, M; Kawai, Y; Watanabe, K; Iri, H

    1989-07-01

    We have examined the influence of erythrocytes containing inclusion bodies, nucleated red cells or giant platelets on the measurement of reticulocyte count by automated machine, R-1000. Correlation of the reticulocyte count between automated and conventional method was extremely good in the blood containing red cells with Jolly bodies, Pappenheimer bodies or basophilic stippling . However, correlation was poor when the sample contained the nucleated red cells. Reticulocyte count was decreased in the blood with significant amounts of nucleated red cells. Since nucleated red cells themselves are not counted as reticulocytes in the machine, this was considered to be due to increased young reticulocytes which frequently appeared with nucleated red cells. Both cold agglutinated red cells and giant platelets apparently influenced the reticulocyte count by the R-1000. These results suggest that red cells with Jolly bodies, Pappenheimer bodies or basophilic stippling do not influence the automatic counting of reticulocytes. Although nucleated red cells, cold agglutinated red cells and giant platelets affected the reticulocyte count, the machine shows abnormal flags in most of above cases (except highly agglutinated red cells), so that one can recount reticulocytes by conventional method. We conclude the machine can safely count the reticulocytes even in the blood containing abnormal red cells or platelets.

  1. Automated blood cell count: a sensitive and reliable method to study corticosterone-related stress in broilers.

    NARCIS (Netherlands)

    Post, J.; Rebel, J.M.J.; Huurne, ter A.A.H.M.

    2003-01-01

    In chickens the heterophil/lymphocyte ratio (H/L) has proved to be a valuable tool in stress related research. In general, H/L is determined with the microscopic differential count on a blood film. We evaluated automated analysis for measuring blood cell parameters in relation to corticosterone in a

  2. Components of the complete blood count as risk predictors for coronary heart disease: in-depth review and update.

    Science.gov (United States)

    Madjid, Mohammad; Fatemi, Omid

    2013-01-01

    Atherosclerosis is an inflammatory disease, and several inflammatory biomarkers, such as C-reactive protein, have been used to predict the risk of coronary heart disease. High white blood cell count is a strong and independent predictor of coronary risk in patients of both sexes, with and without coronary heart disease. A high number of white blood cells and their subtypes (for example, neutrophils, monocytes, lymphocytes, and eosinophils) are associated with the presence of coronary heart disease, peripheral arterial disease, and stroke. The coronary heart disease risk ratios associated with a high white blood cell count are comparable to those of other inflammatory markers, including C-reactive protein. In addition, other components of the complete blood count, such as hematocrit and the erythrocyte sedimentation rate, also are associated with coronary heart disease, and the combination of the complete blood count with the white blood cell count can improve our ability to predict coronary heart disease risk. These tests are inexpensive, widely available, and easy to order and interpret. They merit further research.

  3. Plasma sE-selectin level is positively correlated with neutrophil count and diastolic blood pressure in Japanese men.

    Science.gov (United States)

    Mochizuki, Kazuki; Inoue, Seiya; Miyauchi, Rie; Misaki, Yasumi; Shimada, Masaya; Kasezawa, Nobuhiko; Tohyama, Kazushige; Goda, Toshinao

    2013-01-01

    Increased levels of circulating soluble type of E-selectin (sE-selectin), neutrophil counts and blood pressure are associated with the development of cardiovascular diseases (CVD). In this study, we conducted a cross-sectional study of men who participated in health check-ups, and selected those who were not diagnosed with or being treated for metabolic diseases such as diabetes, hypertension and lipid abnormality according to the health check-ups. We measured their basic clinical parameters including blood pressure and neutrophil count, plasma sE-selectin concentration and lifestyle factors, and assessed their interrelations by multivariate linear regression (MLR) analysis. A total of 351 subjects aged 47.5±8.41 (range, 30-64) y were recruited. Significantly correlated with sE-selectin concentration were neutrophil count, diastolic blood pressure (DBP), and systolic blood pressure (SBP) (Pearson's correlation coefficient, 0.194, 0.220 and 0.175, respectively). MLR analysis showed that sE-selectin concentration was independently positively related with DBP and neutrophil count, whereas neutrophil count was positively associated with sE-selectin concentration but not DBP. DBP, but not SBP, was independently positively correlated with sE-selectin concentration but not neutrophil count. These results indicate that circulating sE-selectin concentration may be a biomarker for indicating subsequent development of metabolic diseases, in particular CVD, from a healthy state.

  4. Acute effects of second-hand smoke on complete blood count.

    Science.gov (United States)

    Dinas, Petros C; Metsios, Giorgos S; Jamurtas, Athanasios Z; Tzatzarakis, Manolis N; Wallace Hayes, A; Koutedakis, Yiannis; Tsatsakis, Aristidis M; Flouris, Andreas D

    2014-01-01

    We assessed the acute effects of a 1-h exposure to second-hand smoke (SHS) on complete blood count (CBC) markers in a controlled simulated bar/restaurant environment. Nineteen adult never-smokers completed a 1-h .exposure to SHS at bar/restaurant levels, and a 1-h exposure to normal room air. Blood samples were collected at the baseline at 30 min during each exposure, and at 0, 0.5, 1, 2, 3, and 4 h after each exposure. The values of white blood cells (WBC) at 1 h (p = 0.010), 3 h (p = 0.040), and 4 h (p = 0.008) following SHS were significantly increased compared with the baseline values. Also, there was a positive association between the WBC and cotinine levels (r = 0.28, p = 0.007). A 1-h exposure to SHS at bar/restaurant levels significantly increased the WBC for at least 4 h following the exposure time. This effect of SHS on WBC has dose-response characteristics and should be considered to prescribing CBC.

  5. A more appropriate white blood cell count for estimating malaria parasite density in Plasmodium vivax patients in northeastern Myanmar.

    Science.gov (United States)

    Liu, Huaie; Feng, Guohua; Zeng, Weilin; Li, Xiaomei; Bai, Yao; Deng, Shuang; Ruan, Yonghua; Morris, James; Li, Siman; Yang, Zhaoqing; Cui, Liwang

    2016-04-01

    The conventional method of estimating parasite densities employ an assumption of 8000 white blood cells (WBCs)/μl. However, due to leucopenia in malaria patients, this number appears to overestimate parasite densities. In this study, we assessed the accuracy of parasite density estimated using this assumed WBC count in eastern Myanmar, where Plasmodium vivax has become increasingly prevalent. From 256 patients with uncomplicated P. vivax malaria, we estimated parasite density and counted WBCs by using an automated blood cell counter. It was found that WBC counts were not significantly different between patients of different gender, axillary temperature, and body mass index levels, whereas they were significantly different between age groups of patients and the time points of measurement. The median parasite densities calculated with the actual WBC counts (1903/μl) and the assumed WBC count of 8000/μl (2570/μl) were significantly different. We demonstrated that using the assumed WBC count of 8000 cells/μl to estimate parasite densities of P. vivax malaria patients in this area would lead to an overestimation. For P. vivax patients aged five years and older, an assumed WBC count of 5500/μl best estimated parasite densities. This study provides more realistic assumed WBC counts for estimating parasite densities in P. vivax patients from low-endemicity areas of Southeast Asia.

  6. Effect of inhaled corticosteroids on blood eosinophil count in steroid-naïve patients with COPD

    OpenAIRE

    Kreindler, James L.; Watkins, Michael L; Lettis, Sally; Tal-Singer, Ruth; Locantore, Nicholas

    2016-01-01

    Introduction Sputum and blood eosinophil counts have attracted attention as potential biomarkers in chronic obstructive pulmonary disease (COPD). One question regarding the use of blood eosinophils as a biomarker in COPD is whether their levels are affected by the use of inhaled corticosteroids (ICS), which are commonly prescribed for COPD. Methods We performed a retrospective analysis of peripheral blood leucocytes from a previously completed clinical trial that examined effects of ICS in st...

  7. A MEMS Complete Blood Count Sensor with Vanes for Reduction in Influence of Electrolysis Gas

    Science.gov (United States)

    Tanabe, Rikiya; Hata, Seiichi; Shimokohbe, Akira

    To develop a miniature complete blood count (CBC) analyzer for point-of-care testing (POCT), a MEMS CBC sensor based on the impedance method is discussed. A novel MEMS CBC sensor that is fabricated through a simple photolithography process using SU-8 is realized. However, the fabricated sensor exhibits a noisy output signal due to electrolysis gas. The relationship between the noise and the gas is revealed through microscopic observation and finite element method (FEM) simulation. To solve the problem of electrolysis gas, an improved MEMS CBC sensor with vanes is developed. The improved sensor is unaffected by electrolysis gas. Moreover, the signal stability of the sensor and the signals detected for latex particles are successfully evaluated.

  8. Automated counting of morphologically normal red blood cells by using digital holographic microscopy and statistical methods

    Science.gov (United States)

    Moon, Inkyu; Yi, Faliu

    2015-09-01

    In this paper we overview a method to automatically count morphologically normal red blood cells (RBCs) by using off-axis digital holographic microscopy and statistical methods. Three kinds of RBC are used as training and testing data. All of the RBC phase images are obtained with digital holographic microscopy (DHM) that is robust to transparent or semitransparent biological cells. For the determination of morphologically normal RBCs, the RBC's phase images are first segmented with marker-controlled watershed transform algorithm. Multiple features are extracted from the segmented cells. Moreover, the statistical method of Hotelling's T-square test is conducted to show that the 3D features from 3D imaging method can improve the discrimination performance for counting of normal shapes of RBCs. Finally, the classifier is designed by using statistical Bayesian algorithm and the misclassification rates are measured with leave-one-out technique. Experimental results show the feasibility of the classification method for calculating the percentage of each typical normal RBC shape.

  9. The Effect of Taraxacum Officinale Hydro Alcoholic Extract on the Blood Cell Counts in Mice

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    m Modaresi

    2012-12-01

    Full Text Available Abstract Background & aim: Taraxacum officinaleis a herbaceous perennial plant which has many pharmaceutical effects. The aim of this study was to investigate the effect of hydro-alcoholic extract of this plant on blood cell counts in mice. Methods: In this experimental study, 50 mature female mice were divided into 5 groups, each group including ten adult female Balb/C mice. The control group did not receive any extract.while the placebo group received 0.5 cc of normal saline, every other day. The three treatment groups intraperitoneally received 50, 100, 200 mg/kg /2day doses of hydro alcoholic extract for 20 days. Normal saline was administered to the control group.WBC, RBC, HB, HCT, platelet and other cells of the animals were counted using full automated cell counter. Data were analyzed by one-way ANOVA. Results: The number of RBC and the rate of Hb in three doses of 50, 100 and 200 mg/kg were significantly increased (p<0.05 in all three treatment groups as compared with the control group. The number of WBC in three doses of 50, 100 and 200 mg/kg increased, but it was significant in 200 mg/kg dandelion treated group as compared with the control group (p<0.05.The rate of platelet in three doses of 50, 100 and 200 mg/kg significantly decreased as compared with the control group (p<0.01. Conclusion: The study confirmed the dose dependent efficacy of dandelion extract on RBC and WBC. Keywords: Dandelion, Blood Cell, mice

  10. Current cigarette smoking is a reversible cause of elevated white blood cell count: Cross-sectional and longitudinal studies.

    Science.gov (United States)

    Higuchi, Takakazu; Omata, Fumio; Tsuchihashi, Kenji; Higashioka, Kazuhiko; Koyamada, Ryosuke; Okada, Sadamu

    2016-12-01

    While cigarette smoking is a well-recognized cause of elevated white blood cell (WBC) count, studies on longitudinal effect of smoking cessation on WBC count are limited. We attempted to determine causal relationships between smoking and elevated WBC count by retrospective cross-sectional study consisting of 37,972 healthy Japanese adults who had a health check-up between April 1, 2008 and March 31, 2009 and longitudinal study involving 1730 current smokers who had more than four consecutive annual health check-ups between April 1, 2007 and March 31, 2012. In the cross-sectional study, younger age, male gender, increased body mass index, no alcohol habit, current smoking, and elevated C-reactive protein level were associated with elevated WBC count. Among these factors, current smoking had the most significant association with elevated WBC count. In subgroup analyses by WBC differentials, smoking was significantly associated with elevated counts of neutrophils, lymphocytes, monocytes, eosinophils, and basophils. Ex-smoking was not associated with elevated WBC count. In the longitudinal study, both WBC and neutrophil counts decreased significantly in one year after smoking cessation and remained down-regulated for longer than next two years. There was no significant change in either WBC or neutrophil count in those who continued smoking. These findings clearly demonstrated that current smoking is strongly associated with elevated WBC count and smoking cessation leads to recovery of WBC count in one year, which is maintained for longer than subsequent two years. Thus, current smoking is a significant and reversible cause of elevated WBC count in healthy adults.

  11. Determination of reference ranges for full blood count parameters in neonatal cord plasma in Hilla, Babil, Iraq

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    Al-Marzoki JM

    2012-10-01

    Full Text Available Jasim M Al-Marzoki1, Zainab W Al-Maaroof2, Ali H Kadhum31Department of Pediatrics, 2Department of Pathology, Babylon Medical College, 3Babylon Gynecology and Pediatric Teaching Hospital, Hilla, IraqBackground: The health of an individual is known to vary in different countries, in the same country at different times, and in the same individuals at different ages. This means that the condition of individuals must be related to or compared with reference data. Determination of a reference range for the healthy term newborn is clinically important in terms of various complete blood count parameters. The purpose of this study was to establish a local reference range for full blood count parameters in neonatal cord plasma in Hilla, Babil, Iraq.Methods: A total of 220 mothers and their neonates were enrolled in this cross-sectional study from February 2011 to January 2012. Maternal inclusion criteria were age 15–45 years, an uneventful pregnancy, and hemoglobin ≥ 10 g. Neonatal inclusion criteria were full term (37–42 weeks and normal birth weight. The umbilical cord was immediately clamped after delivery of the baby; 3 mL of cord blood was then taken from the umbilical vein and collected in a tube containing ethylenediamine tetra-acetic acid, its plasma was analyzed for full blood count parameters by standard Coulter gram, and the differential leukocyte count was done manually.Results: Mean neonatal hemoglobin was 13.88 ± 1.34 (range 11–17.3 g/dL and mean white cell count was 10.12 ± 2.8 (range 3.1–21.6 × 109/L. Mean platelet count was 267.63 ± 60.62 (range 152–472 × 109/L. No significant differences in red cell, white cell, or platelet counts were found between males and females, except for neutrophil count. The current study shows lower levels of hemoglobin, white cells, and red cells compared with other studies, and there is agreement with some studies and disagreement with others concerning platelet count.Conclusion: Most results

  12. The changes in complete blood count in thyroid cancer patients treated with radioactive iodine ablation therapy

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    Bircan Sönmez

    2010-12-01

    Full Text Available Objective: The aim of this study was to evaluate the effect of radioactive iodine (RAI ablation therapy on the complete blood count (CBC in thyroid cancer patients. Materials and Methods: One hundred sixty four patients undergoing RAI ablation therapy after total thyroidectomy were included. CBC results were available from the patients’ medical records at the time of ablation and at the 1st, 6th, and 12th months after RAI therapy.Results: Hemoglobin (Hb, white blood cell (WBC and platelet (Plt values were significantly lower than baseline at 1 month after treatment (p<0.0001. Hb and WBC values were increased at the 6th month and at the 1st year. Plt values increased at the 6th month but had decreased again at the 1st year. The values were usually in normal ranges except in the patients with low pretreatment Hb and WBC values.Conclusion: RAI ablation therapy in thyroid cancer patients is a safe treatment modality without any serious or persistent hematological side effects.

  13. Automated screening for myelodysplastic syndromes through analysis of complete blood count and cell population data parameters.

    Science.gov (United States)

    Raess, Philipp W; van de Geijn, Gert-Jan M; Njo, Tjin L; Klop, Boudewijn; Sukhachev, Dmitry; Wertheim, Gerald; McAleer, Tom; Master, Stephen R; Bagg, Adam

    2014-04-01

    The diagnosis of myelodysplastic syndromes (MDS) requires a high clinical index of suspicion to prompt bone marrow studies as well as subjective assessment of dysplastic morphology. We sought to determine if data collected by automated hematology analyzers during complete blood count (CBC) analysis might help to identify MDS in a routine clinical setting. We collected CBC parameters (including those for research use only and cell population data) and demographic information in a large (>5,000), unselected sequential cohort of outpatients. The cohort was divided into independent training and test groups to develop and validate a random forest classifier that identifies MDS. The classifier effectively identified MDS and had a receiver operating characteristic area under the curve (AUC) of 0.942. Platelet distribution width and the standard deviation of red blood cell distribution width were the most discriminating variables within the classifier. Additionally, a similar classifier was validated with an additional, independent set of >200 patients from a second institution with an AUC of 0.93. This retrospective study demonstrates the feasibility of identifying MDS in an unselected outpatient population using data routinely collected during CBC analysis with a classifier that has been validated using two independent data sets from different institutions.

  14. Straw blood cell count, growth, inhibition and comparison to apoptotic bodies

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    Tomkins Jeffrey P

    2008-05-01

    Full Text Available Abstract Background Mammalian cells transform into individual tubular straw cells naturally in tissues and in response to desiccation related stress in vitro. The transformation event is characterized by a dramatic cellular deformation process which includes: condensation of certain cellular materials into a much smaller tubular structure, synthesis of a tubular wall and growth of filamentous extensions. This study continues the characterization of straw cells in blood, as well as the mechanisms of tubular transformation in response to stress; with specific emphasis placed on investigating whether tubular transformation shares the same signaling pathway as apoptosis. Results There are approximately 100 billion, unconventional, tubular straw cells in human blood at any given time. The straw blood cell count (SBC is 45 million/ml, which accounts for 6.9% of the bloods dry weight. Straw cells originating from the lungs, liver and lymphocytes have varying nodules, hairiness and dimensions. Lipid profiling reveals severe disruption of the plasma membrane in CACO cells during transformation. The growth rates for the elongation of filaments and enlargement of rabbit straw cells is 0.6~1.1 (μm/hr and 3.8 (μm3/hr, respectively. Studies using apoptosis inhibitors and a tubular transformation inhibitor in CACO2 cells and in mice suggested apoptosis produced apoptotic bodies are mediated differently than tubular transformation produced straw cells. A single dose of 0.01 mg/kg/day of p38 MAPK inhibitor in wild type mice results in a 30% reduction in the SBC. In 9 domestic animals SBC appears to correlate inversely with an animal's average lifespan (R2 = 0.7. Conclusion Straw cells are observed residing in the mammalian blood with large quantities. Production of SBC appears to be constant for a given animal and may involve a stress-inducible protein kinase (P38 MAPK. Tubular transformation is a programmed cell survival process that diverges from apoptosis

  15. Peripheral blood CD34+ cell count as a predictor of adequacy of hematopoietic stem cell collection for autologous transplantation

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    Combariza, Juan F.

    2016-10-01

    Full Text Available Introduction: In order to carry out an autologous transplantation, hematopoietic stem cells should be mobilized to peripheral blood and later collected by apheresis. The CD34+ cell count is a tool to establish the optimal time to begin the apheresis procedure. Objective: To evaluate the association between peripheral blood CD34+ cell count and the successful collection of hematopoietic stem cells. Materials and methods: A predictive test evaluation study was carried out to establish the usefulness of peripheral blood CD34+ cell count as a predictor of successful stem cell collection in patients that will receive an autologous transplantation. Results: 77 patients were included (median age: 49 years; range: 5-66. The predominant baseline diagnosis was lymphoma (53.2 %. The percentage of patients with successful harvest of hematopoietic stem cells was proportional to the number of CD34+cells in peripheral blood at the end of the mobilization procedure. We propose that more than 15 CD34+cells/μL must be present in order to achieve an adequate collection of hematopoietic stem cells. Conclusion: Peripheral blood CD34+ cell count is a useful tool to predict the successful collection of hematopoietic stem cells.

  16. Red blood cell count as an indicator of microvascular complications in Chinese patients with type 2 diabetes mellitus

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    Wang ZS

    2013-05-01

    Full Text Available Zhan-Sheng Wang,1,2 Zhan-Chun Song,1 Jing-Hui Bai,1 Fei Li,3 Tao Wu,1 Ji Qi,2 Jian Hu11Department of Cardiology, First Affiliated Hospital of China Medical University, 2Second Department of Cardiology, Fourth People's Hospital of Shenyang, Shenyang, 3Department of Cardiology, Shenzhou Hospital of Shenyang Medical College, Shenyang, People's Republic of ChinaBackground: Rheological disorders of red blood cells (RBC and decreased RBC deformability have been involved in the development of diabetic microangiopathy. However, few studies have evaluated the association of RBC count with microvascular complications in patients with type 2 diabetes mellitus (T2DM. The purpose of this study was to investigate the association of RBC count with microvascular complications in patients with T2DM.Methods: This study involved 369 patients with T2DM: 243 with one or more microvascular complications and 126 without microvascular complications. Anticoagulated blood was collected and analyzed in an automated blood cell counter. The presence of risk factors for microvascular complications was determined.Results: The proportion of patients with microvascular complications increased as the RBC count decreased (P < 0.001. After adjustment for known risk factors for microvascular complications by logistic regression analysis, lower quartiles of RBC count were associated with a higher risk of microvascular complications compared with the reference group composed of the highest quartile (first quartile, odds ratio 4.98, 95% confidence interval 1.54–6.19, P = 0.008; second quartile, odds ratio 3.21, 95% confidence interval 1.17–5.28, P = 0.024.Conclusion: A decreased RBC count is associated with microvascular complications in Chinese patients with T2DM. The RBC count is a potential marker to improve further the ability to identify diabetic patients at high risk of microvascular complications.Keywords: red blood cell count, microvascular complication, type 2 diabetes

  17. Predictive Value of Nucleated Red Blood Cell Counts in Cord and Peripheral Blood of Asphyxiated Term Neonates in the First Week of Life

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    B Bahman Bijari

    2010-03-01

    Full Text Available Introduction: Increased numbers of nucleated red blood cells (NRBC circulating in the blood of neonates can be associated with relative hypoxia and adverse outcomes. Thus, the aim of this study was to assess the NRBC count during the first week of life in neonates diagnosed with asphyxia as compared to healthy neonates and to determine the short-term morbidity and mortality for the affected babies. Methods: The cross-sectional study compared 15 healthy neonates with 15 neonates diagnosed with asphyxia confirmed by pH of cord blood or Apgar scores. The nucleated red blood cell (NRBC counts were calculated right after birth, and on days 3 and 7, and the hematological parameters of umbilical cord blood were also evaluated. The infants were followed for mortality and associated morbidity. Statistical analysis was conducted using the Mann-Whitney U test, analysis of variance, chi-square tests, and Pearson’s correlation coefficient. A p-value < 0.05 was considered as statistically significant. Results: The initial NRBC counts were significantly higher in the asphyxiated group than in the control group and the difference remained significant through the end of first week. All of the umbilical cord blood parameters were significantly lower in the study group and were negatively correlated with the NRBC count. At birth, higher NRBC count correlated with higher mortality. conclution: Results show that NRBC count is a useful predictive factor for neonatal asphyxia through the end of the first week of life, although a larger study population and a longer follow up period seems to be necessary.

  18. Intravenous Tissue Plasminogen Activator Can Be Safely Given without Complete Blood Count Results Back

    Science.gov (United States)

    Dong, Yi; Yang, Lumeng; Ren, Jinma; Nair, Deepak S.; Parker, Sarah; Jahnel, Jan L.; Swanson-Devlin, Teresa G.; Beck, Judith M.; Mathews, Maureen; McNeil, Clayton J.; Ling, Yifeng; Cheng, Xin; Gao, Yuan; Dong, Qiang; Wang, David Z.

    2015-01-01

    Introduction It is well known that the efficacy of intravenous (IV) tissue plasminogen activator (tPA) is time-dependent when used to treat patients with acute ischemic strokes. Aim Our study examines the safety issue of giving IV tPA without complete blood count (CBC) resulted. Materials and Methods This is a retrospective observational study by examining the database from Huashan Hospital in China and OSF/INI Comprehensive Stroke Center in United States. Patient data collected included demographics, occurrence of symptomatic intracranial hemorrhage, door to needle intervals, National Institute of Health Stroke Scale scores on admission, CBC results on admission and follow-up modified Rankin Scale scores. Linear regression and multivariable logistic regression analysis were used to identify factors that would have an impact on door-to-needle intervals. Results Our study included120 patients from Huashan Hospital and 123 patients from INI. Among them, 36 in Huashan Hospital and 51in INI received IV tPA prior to their CBC resulted. Normal platelet count was found in 98.8% patients after tPA was given. One patient had thrombocytopenia but no hemorrhagic event. A significantly shorter door to needle interval (DTN) was found in the group without CBC resulted. There was also a difference in treatment interval between the two hospitals. Door to needle intervals had a strong correlation to onset to treatment intervals and NIHSS scores on admission. Conclusion In patients presented with acute ischemic stroke, the risk of developing hemorrhagic event is low if IV tPA is given before CBC has resulted. The door to needle intervals can be significantly reduced. PMID:26147994

  19. Intravenous Tissue Plasminogen Activator Can Be Safely Given without Complete Blood Count Results Back.

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    Yi Dong

    Full Text Available It is well known that the efficacy of intravenous (i.v. tissue plasminogen activator (tPA is time-dependent when used to treat patients with acute ischemic strokes.Our study examines the safety issue of giving IV tPA without complete blood count (CBC resulted.This is a retrospective observational study by examining the database from Huashan Hospital in China and OSF/INI Comprehensive Stroke Center in United States. Patient data collected included demographics, occurrence of symptomatic intracranial hemorrhage, door to needle intervals, National Institute of Health Stroke Scale scores on admission, CBC results on admission and follow-up modified Rankin Scale scores. Linear regression and multivariable logistic regression analysis were used to identify factors that would have an impact on door-to-needle intervals.Our study included 120 patients from Huashan Hospital and 123 patients from INI. Among them, 36 in Huashan Hospital and 51 in INI received i.v. tPA prior to their CBC resulted. Normal platelet count was found in 98.8% patients after tPA was given. One patient had thrombocytopenia but no hemorrhagic event. A significantly shorter door to needle interval (DTN was found in the group without CBC resulted. There was also a difference in treatment interval between the two hospitals. Door to needle intervals had a strong correlation to onset to treatment intervals and NIHSS scores on admission.In patients presented with acute ischemic stroke, the risk of developing hemorrhagic event is low if i.v. tPA is given before CBC has resulted. The door to needle intervals can be significantly reduced.

  20. The association of sensitization to inhalant allergens with allergy symptoms : the influence of bronchial hyperresponsiveness and blood eosinophil count

    NARCIS (Netherlands)

    Kerkhof, M; Schouten, JP; De Monchy, JGR

    2000-01-01

    Background We investigated whether the association of allergy symptoms with sensitization to inhalant allergens depends on bronchial hyperresponsiveness, blood eosinophil count, or the degree and nature of sensitization. Methods Data on asthma and rhino-conjunctivitis symptoms were obtained from 190

  1. Development of a preliminary diagnostic measure for bovine leukosis in dairy cows using peripheral white blood cell and lymphocyte counts

    Science.gov (United States)

    NISHIIKE, Masao; HAOKA, Michiyo; DOI, Takashi; KOHDA, Tomoko; MUKAMOTO, Masafumi

    2016-01-01

    Analysis of the association between antibodies against bovine leukemia virus (BLV), BLV proviral load, and white blood cell (WBC) and lymphocyte counts was performed with 774 dairy cows. The average age, WBC counts and lymphoid cell counts tended to be higher in BLV antibody-positive cows than in antibody-negative cows. There was a similar trend in levels of proviral DNA. We analyzed age, WBC counts and lymphocyte counts by principal component analyses to create a distribution chart of the principle component scores. Using the chart, we categorized cows into four quadrants based on additional information, such as the presence of antibody and the levels of proviral DNA. Antibody-positive cows and cows with high BLV proviral load were found mostly in one quadrant of the chart, indicating that it is possible to predict the risk of infection without any knowledge on antibody status by using information, such as WBC counts as a biomarker. When only antibody-positive cows were included in the analysis, a characteristic distribution of different levels of proviral DNA was seen in the quadrants, suggesting that it is possible to estimate the extent of bovine leukosis infection by using this analysis. For this analysis and categorization of the cows into quadrants, we computed a mathematical formulation using discriminant analysis based on age and WBC and lymphocyte counts. This mathematical formulation for the hematological preliminary diagnosis of the disease is recommended as a screening tool to monitor bovine leukosis. PMID:27064146

  2. Red blood cell count has an independent contribution to the prediction of ultrasonography-diagnosed fatty liver disease

    Science.gov (United States)

    Wu, Shang-ling; Liao, Gong-cheng; Fang, Ai-ping; Zhu, Ming-fan; Zhu, Hui-lian

    2017-01-01

    Background & aims Red blood cell (RBC) indices have been demonstrated to be associated with fatty liver disease (FLD) and metabolic syndrome. However, controversy exists regarding the relationship of RBC indices with FLD to date and few has focused on RBC count. This study aimed to explore the association between RBC count and risk of FLD in Southern Chinese adults. Methods A hospital-based cross-sectional study was performed in two hospital health examination centers, including information on ultrasonography-diagnosed FLD, anthropometric indices and biochemical measurements. Covariance analysis was used to evaluate group differences. After quintile classification of RBC counts, logistic regression analysis was conducted to evaluate the odds ratios (ORs) of FLD. Results This study consisted of 8618 subjects (4137 men and 4481 women) aged between 20 and 89 years. FLD cases had higher RBC counts than non-FLD cases in both genders (Pwomen. Binary logistic regression analysis showed positive association between RBC count and FLD, and the OR (95% confidence interval (CI)) were 2.56 (2.06–3.18) in men and 3.69 (2.74–4.98) in women, respectively, when comparing Q5 with Q1. Stratified analyses showed similar trends among subjects with and without FLD risk factors. Gender independent results were similar to gender dependent results. Conclusions Elevated RBC count is independently associated with high risk of FLD, suggesting that the RBC count may be a potential risk predictor for FLD. PMID:28187211

  3. Flow cytometric assessment of activation of peripheral blood platelets in dogs with normal platelet count and asymptomatic thrombocytopenia.

    Science.gov (United States)

    Żmigrodzka, M; Guzera, M; Winnicka, A

    2016-01-01

    Platelets play a crucial role in hemostasis. Their activation has not yet been evaluated in healthy dogs with a normal and low platelet count. The aim of this study was to determine the influence of activators on platelet activation in dogs with a normal platelet count and asymptomatic thrombocytopenia. 72 clinically healthy dogs were enrolled. Patients were allocated into three groups. Group 1 consisted of 30 dogs with a normal platelet count, group 2 included 22 dogs with a platelet count between 100 and 200×109/l and group 3 consisted of 20 dogs with a platelet count lower than 100×109/l. Platelet rich-plasma (PRP) was obtained from peripheral blood samples using tripotassium ethylenediaminetetraacetic acid (K3-EDTA) as anticoagulant. Next, platelets were stimulated using phorbol-12-myristate-13-acetate or thrombin, stabilized using procaine or left unstimulated. The expression of CD51 and CD41/CD61 was evaluated. Co-expression of CD41/CD61 and Annexin V served as a marker of platelet activation. The expression of CD41/CD61 and CD51 did not differ between the 3 groups. Thrombin-stimulated platelets had a significantly higher activity in dogs with a normal platelet count than in dogs with asymptomatic thrombocytopenia. Procaine inhibited platelet activity in all groups. In conclusion, activation of platelets of healthy dogs in vitro varied depending on the platelet count and platelet activator.

  4. Cellular softening mediates leukocyte demargination and trafficking, thereby increasing clinical blood counts.

    Science.gov (United States)

    Fay, Meredith E; Myers, David R; Kumar, Amit; Turbyfield, Cory T; Byler, Rebecca; Crawford, Kaci; Mannino, Robert G; Laohapant, Alvin; Tyburski, Erika A; Sakurai, Yumiko; Rosenbluth, Michael J; Switz, Neil A; Sulchek, Todd A; Graham, Michael D; Lam, Wilbur A

    2016-02-23

    Leukocytes normally marginate toward the vascular wall in large vessels and within the microvasculature. Reversal of this process, leukocyte demargination, leads to substantial increases in the clinical white blood cell and granulocyte count and is a well-documented effect of glucocorticoid and catecholamine hormones, although the underlying mechanisms remain unclear. Here we show that alterations in granulocyte mechanical properties are the driving force behind glucocorticoid- and catecholamine-induced demargination. First, we found that the proportions of granulocytes from healthy human subjects that traversed and demarginated from microfluidic models of capillary beds and veins, respectively, increased after the subjects ingested glucocorticoids. Also, we show that glucocorticoid and catecholamine exposure reorganizes cellular cortical actin, significantly reducing granulocyte stiffness, as measured with atomic force microscopy. Furthermore, using simple kinetic theory computational modeling, we found that this reduction in stiffness alone is sufficient to cause granulocyte demargination. Taken together, our findings reveal a biomechanical answer to an old hematologic question regarding how glucocorticoids and catecholamines cause leukocyte demargination. In addition, in a broader sense, we have discovered a temporally and energetically efficient mechanism in which the innate immune system can simply alter leukocyte stiffness to fine tune margination/demargination and therefore leukocyte trafficking in general. These observations have broad clinically relevant implications for the inflammatory process overall as well as hematopoietic stem cell mobilization and homing.

  5. Aging stability of complete blood count and white blood cell differential parameters analyzed by Abbott CELL-DYN Sapphire hematology analyzer.

    Science.gov (United States)

    Hedberg, P; Lehto, T

    2009-02-01

    This study presents the results of an aging stability study of complete blood count (CBC) and leukocyte differential parameters using the Abbott CELL-DYN Sapphire hematology analyzer. Stability studies showed no substantial change in CBC parameters up to 24-48 h at +23 +/- 2 degrees C (room temperature), except for optical platelet count (PLTo). For specimens aged over 24, the value of impedance platelet count yielded more reliable results than the routine PLTo. White blood cell (WBC) differential parameters, except eosinophils, were stable for up to 48 h at +23 +/- 2 degrees C. CBC parameters were stable for 72 h, except mean platelet volume, which slightly increased between 48 and 72 h, at +4 degrees C. WBC differentials were stable 48-72 h, with a slight decrease observed in absolute neutrophils and lymphocytes at +4 degrees C.

  6. The effect of pneumatic tube system on complete blood count parameters and thrombocyte donation in healthy donors.

    Science.gov (United States)

    Sari, Ismail; Arslan, Aliriza; Ozlu, Can; Hacioglu, Sibel; Dogu, Mehmet Hilmi; Isler, Kamil; Keskin, Ali

    2012-08-01

    This paper is the first report whether or not pneumatic tube system affects the selection of apheresis donors according to the results of complete blood count. According to the apheresis guidelines, hemoglobin level must be ≥12.5g/dL and platelet level ≥150/μL to be a donor. Paired blood samples of 26 healthy volunteers were transported by either hand delivered or a pneumatic tube system to the laboratory. No statistically significant differences were observed in order to mean values of routine complete blood cell count and white cell differential parameters that were send for selection of apheresis donor before the procedure. Therefore, all healthy volunteers decided as a donor according to the laboratory results independent from transport method.

  7. Three-dimensional counting of morphologically normal human red blood cells via digital holographic microscopy

    Science.gov (United States)

    Yi, Faliu; Moon, Inkyu; Lee, Yeon H.

    2015-01-01

    Counting morphologically normal cells in human red blood cells (RBCs) is extremely beneficial in the health care field. We propose a three-dimensional (3-D) classification method of automatically determining the morphologically normal RBCs in the phase image of multiple human RBCs that are obtained by off-axis digital holographic microscopy (DHM). The RBC holograms are first recorded by DHM, and then the phase images of multiple RBCs are reconstructed by a computational numerical algorithm. To design the classifier, the three typical RBC shapes, which are stomatocyte, discocyte, and echinocyte, are used for training and testing. Nonmain or abnormal RBC shapes different from the three normal shapes are defined as the fourth category. Ten features, including projected surface area, average phase value, mean corpuscular hemoglobin, perimeter, mean corpuscular hemoglobin surface density, circularity, mean phase of center part, sphericity coefficient, elongation, and pallor, are extracted from each RBC after segmenting the reconstructed phase images by using a watershed transform algorithm. Moreover, four additional properties, such as projected surface area, perimeter, average phase value, and elongation, are measured from the inner part of each cell, which can give significant information beyond the previous 10 features for the separation of the RBC groups; these are verified in the experiment by the statistical method of Hotelling's T-square test. We also apply the principal component analysis algorithm to reduce the dimension number of variables and establish the Gaussian mixture densities using the projected data with the first eight principal components. Consequently, the Gaussian mixtures are used to design the discriminant functions based on Bayesian decision theory. To improve the performance of the Bayes classifier and the accuracy of estimation of its error rate, the leaving-one-out technique is applied. Experimental results show that the proposed method can

  8. Systemic inflammation in 222.841 healthy employed smokers and nonsmokers: white blood cell count and relationship to spirometry

    Directory of Open Access Journals (Sweden)

    Fernández José Antonio

    2012-05-01

    Full Text Available Abstract Background Smoking has been linked to low-grade systemic inflammation, a known risk factor for disease. This state is reflected in elevated white blood cell (WBC count. Objective We analyzed the relationship between WBC count and smoking in healthy men and women across several age ranges who underwent preventive medical check-ups in the workplace. We also analysed the relationship between smoking and lung function. Methods Cross-sectional descriptive study in 163 459 men and 59 382 women aged between 16 and 70 years. Data analysed were smoking status, WBC count, and spirometry readings. Results Total WBC showed higher counts in both male and female smokers, around 1000 to 1300 cell/ml (t test, P 1% was higher in nonsmokers for both sexes between 25 to 54 years (t test, P 1% were found to have higher WBC counts, in comparison to smokers with a normal FEV1% among similar age and BMI groups. Conclusions Smoking increases WBC count and affects lung function. The effects are evident across a wide age range, underlining the importance of initiating preventive measures as soon as an individual begins to smoke.

  9. Complete blood count and acetylcholinesterase activity of lymphocytes of demyelinated and ovariectomized rats treated with resveratrol.

    Science.gov (United States)

    Martins, Danieli B; Mazzanti, Cinthia M; Costa, Márcio M; França, Raqueli; Pagnoncelli, Marcielen; Maciel, Roberto M; Schmatz, Roberta; Oliveira, Lizielle; Morsch, Vera; Facco, Grasiela; Visentini, Diandra; Mann, Thais; Mazzanti, Alexandre; Lopes, Sonia T A

    2012-12-01

    Resveratrol is a phytoestrogen that has many beneficial actions. This study aimed to evaluate the effect of resveratrol on the complete blood count (CBC) and the acetylcholinesterase (AChE) activity of lymphocytes of ovariectomized rats experimentally demyelinated by ethidium bromide (EB). Forty adult female Wistar rats (60 days, 200-220 g) were divided randomly into five groups (n = 4) to evaluate the demyelination phase and five groups (n = 4) to evaluate the remyelination phase. In each phase, the groups consisted of sham rats-G1; ovariectomized rats, not demyelinated, treated only with vehicle (ethanol 25%)-G2; demyelinated ovariectomized rats treated only with vehicle-G3; ovariectomized rats, not demyelinated, treated with resveratrol-G4; and demyelinated ovariectomized rats treated with resveratrol-G5. Only during the remyelination phase, CBC showed a significant difference (p < 0.05) in the number of monocytes between G2 and G5 groups. In the demyelination phase, there was a significant decrease (p < 0.05) in the AChE activity in the G4 group, while the G5 group was statistically similar to the G1, G2 and G4 groups. In the remyelination phase, there were no significant differences in the AChE activity among the groups. The treatment for 7 days with resveratrol with or without the experimental demyelization with EB appears to influence the AChE activity of lymphocytes, without changing the number of these cells in the circulation. However, in the remyelination phase, there seems to be stabilization in its effect on the lymphocyte AChE activity.

  10. Prognostic impact of white blood cell count in intermediate risk acute myeloid leukemia : relevance of mutated NPM1 and FLT3-ITD

    NARCIS (Netherlands)

    de Jonge, Hendrik J. M.; Valk, Peter J. M.; de Bont, Eveline S. J. M.; Schuringa, Jan Jacob; Ossenkoppele, Gert; Vellenga, Edo; Huls, Gerwin

    2011-01-01

    Background High white blood cell count at presentation is an unfavorable prognostic factor for treatment outcome in intermediate cytogenetic risk acute myeloid leukemia. Since the impact of white blood cell count on outcome of subgroups defined by the molecular markers NPMc(+) and FLT3-internal tand

  11. A comparative study on the blood and milk cell counts of healthy, subclinical and clinical mastitis Karan Fries cows

    Directory of Open Access Journals (Sweden)

    Mohanned Alhussien

    2015-05-01

    Full Text Available Aim: The present study was aimed to study the use of cell counts as an early indicator of mammary health. Materials and Methods: Milk and blood cell counts were estimated from 8 healthy, 8 subclinical (SCM, and 8 clinically mastitis (CM groups of Karan Fries (KF cows. Results: Total leucocyte counts and neutrophil percent in blood and milk somatic cells and milk neutrophil percent of healthy cows increased significantly (p<0.05 in SCM cows and CM cows. Viability of blood and milk neutrophils was more in healthy cows, but decreased significantly (p<0.05 in SCM and CM cows. Significant (p<0.05 decrease were also observed in both the blood and milk lymphocytes and monocytes of SCM and CM cows. Phagocytic activity (PA of blood neutrophils also decreased significantly (p<0.05 in SCM cows. There was no difference between the PA of SCM and CM cows. Milk neutrophil percent was more in the SCM and clinically infected milk than in the blood of these cows. About 96-97% of the neutrophils had segmented nucleus in both healthy and subclinical milk, whereas, 2-3% were having band shaped or immature nuclei. There was a significant decrease in the segmented neutrophils, whereas, band neutrophils increase significantly to about 5% in the infected milk of mastitic cows. Viability of the milk neutrophils decreased more in case of subclinical and clinical milk as compared to that of blood. PA was found to be highest in the milk of healthy group of cows, but decreased significantly (p<0.05 in subclinically infected cows. However, there was no difference between the PA of milk neutrophils of SCM and CM cows. PA of milk was also found to be significantly lower in the milk of healthy cows when compared to that of blood neutrophils. Conclusion: This study indicated that percent neutrophils and their type in conjunction with milk somatic cell counts can be used as a more reliable indicator of mammary health in cows.

  12. White blood cell count in women: relation to inflammatory biomarkers, haematological profiles, visceral adiposity, and other cardiovascular risk factors.

    Science.gov (United States)

    Farhangi, Mahdieh Abbasalizad; Keshavarz, Seyyed-Ali; Eshraghian, Mohammadreza; Ostadrahimi, Alireza; Saboor-Yaraghi, Ali-Akbar

    2013-03-01

    The role of white blood cell (WBC) count in pathogenesis of diabetes, cardiovascular disease, and obesity-related disorders has been reported earlier. Recent studies revealed that higher WBC contributes to atherosclerotic progression and impaired fasting glucose. However, it is unknown whether variations in WBC and haematologic profiles can occur in healthy obese individuals. The aim of this study is to further evaluate the influence of obesity on WBC count, inflammatory biomarkers, and metabolic risk factors in healthy women to establish a relationship among variables analyzed. The sample of the present study consisted of 84 healthy women with mean age of 35.56 +/- 6.83 years. They were categorized into two groups based on their body mass index (BMI): obese group with BMI > 30 kg/m2 and non-obese group with BMI count (PLT) with serum interleukin 6 (IL-6), C-reactive protein (CRP), angiotensin pi (Ang pi), body fat percentage (BF %), waist-circumference (WC), and lipid profile. WBC, PLT, CRP, and IL-6 in obese subjects were significantly higher than in non-obese subjects (p count in obese subjects was 6.4 +/- 0.3 (x10(9)/L) compared to 4.4 +/- 0.3 (x10(9)/L) in non-obese subjects (p = 0.035). WBC correlated with BF% (r = 0.31, p = 0.004), CRP (r = 0.25, P = 0.03), WC (r = 0.22, p = 0.04), angiotensin 11 (r = 0.24, p = 0.03), triglyceride (r = 0.24, p = 0.03), and atherogenic index of plasma (AIP) levels (r = 0.3, p = 0.028) but not with IL-6. Platelet count was also associated with WC and waist-to-hip ratio (p count and inflammatory parameters. There was also a positive relationship between WBC count and several inflammatory and metabolic risk factors in healthy women.

  13. Elevated white blood cell count and outcome in cancer patients with venous thromboembolism. Findings from the RIETE Registry.

    Science.gov (United States)

    Trujillo-Santos, Javier; Di Micco, Pierpaolo; Iannuzzo, Mariateresa; Lecumberri, Ramón; Guijarro, Ricardo; Madridano, Olga; Monreal, Manuel

    2008-11-01

    A significant association between elevated white blood cell (WBC) count and mortality in patients with cancer has been reported, but the predictive value of elevated WBC on mortality in cancer patients with acute venous thromboembolism (VTE) has not been explored. RIETE is an ongoing registry of consecutive patients with acute VTE. We compared the three-month outcome of cancer patients with acute VTE according to their WBC count at baseline. As of May 2007, 3805 patients with active cancer and acute VTE had been enrolled in RIETE. Of them, 215 (5.7%) had low- (11,000 cells/microl) WBC count. During the study period 190 patients (5.0%) had recurrent VTE, 156 (4.1%) major bleeding, 889 (23%) died (399 of disseminated cancer, 113 of PE, 46 of bleeding. Patients with elevated WBC count at baseline had an increased incidence of recurrent VTE (odds ratio [OR]: 1.6; 95% confidence interval [CI]: 1.2-2.2), major bleeding (OR: 1.5; 95% CI: 1.1-2.1) or death (OR: 2.7; 95% CI: 2.3-3.2). Most of the reported causes of death were significantly more frequent in patients with elevated WBC count. Multivariate analysis confirmed that elevated WBC count was independently associated with an increased incidence of all three complications. In conclusion, cancer patients with acute VTE and elevated WBC count had an increased incidence of VTE recurrences, major bleeding or death. This worse outcome was consistent among all subgroups and persisted after multivariate adjustment.

  14. Reference range evaluation of complete blood count parameters with emphasis on newer research parameters on the complete blood count analyzer Sysmex XE-2100.

    Science.gov (United States)

    Sehgal, Kunal K; Tina, Dadu; Choksey, Urmi; Dalal, Reeta J; Shanaz, Khodaiji J

    2013-01-01

    Since the advent of automation in the field of hematological cell counters there has been a constant refinement of the technology and increase in the number of newer parameters available on CBC analysers. Many novel parameters are being put into routine clinical use and both clinical evaluation and monitoring critically depend on knowledge of laboratory reference ranges. Here, we present reference interval for the Sysmex XE-2100, with emphasis on the novel or newer research parameters. Blood samples from a total of 122 clinically asymptomatic and apparently healthy subjects were evaluated and a final of 100 subjects (54-M, 46-F) were included in the study. A broad spectrum of parameters available with the analyser was assessed and reference ranges for the same evaluated.

  15. White Blood Cell Counts as Risk Markers of Developing Metabolic Syndrome and Its Components in the Predimed Study

    Science.gov (United States)

    Babio, Nancy; Ibarrola-Jurado, Núria; Bulló, Mònica; Martínez-González, Miguel Ángel; Wärnberg, Julia; Salaverría, Itziar; Ortega-Calvo, Manuel; Estruch, Ramón; Serra-Majem, Lluís; Covas, Maria Isabel; Sorli, José Vicente; Salas-Salvadó, Jordi

    2013-01-01

    Background The Metabolic Syndrome (MetS) is a cluster of metabolic abnormalities that includes hyperglucemia, hypertension, dyslipidemia and central obesity, conferring an increased risk of cardiovascular disease. The white blood cell (WBC) count has been proposed as a marker for predicting cardiovascular risk. However, few prospective studies have evaluated the relationship between WBC subtypes and risk of MetS. Methods Participants were recruited from seven PREDIMED study centers. Both a baseline cross-sectional (n = 4,377) and a prospective assessment (n = 1,637) were performed. Participants with MetS at baseline were excluded from the longitudinal analysis. The median follow-up was 3.9 years. Anthropometric measurements, blood pressure, fasting glucose, lipid profile and WBC counts were assessed at baseline and yearly during the follow-up. Participants were categorized by baseline WBC and its subtype count quartiles. Adjusted logistic regression models were fitted to assess the risk of MetS and its components. Results Of the 4,377 participants, 62.6% had MetS at baseline. Compared to the participants in the lowest baseline sex-adjusted quartile of WBC counts, those in the upper quartile showed an increased risk of having MetS (OR, 2.47; 95%CI, 2.03–2.99; P-trend<0.001). This association was also observed for all WBC subtypes, except for basophils. Compared to participants in the lowest quartile, those in the top quartile of leukocyte, neutrophil and lymphocyte count had an increased risk of MetS incidence. Leukocyte and neutrophil count were found to be strongly associated with the MetS components hypertriglyceridemia and low HDL-cholesterol. Likewise, lymphocyte counts were found to be associated with the incidence of the MetS components low HDL-cholesterol and high fasting glucose. An increase in the total WBC during the follow-up was also associated with an increased risk of MetS. Conclusions Total WBC counts, and some subtypes, were positively

  16. White blood cell counts as risk markers of developing metabolic syndrome and its components in the PREDIMED study.

    Directory of Open Access Journals (Sweden)

    Nancy Babio

    Full Text Available BACKGROUND: The Metabolic Syndrome (MetS is a cluster of metabolic abnormalities that includes hyperglucemia, hypertension, dyslipidemia and central obesity, conferring an increased risk of cardiovascular disease. The white blood cell (WBC count has been proposed as a marker for predicting cardiovascular risk. However, few prospective studies have evaluated the relationship between WBC subtypes and risk of MetS. METHODS: Participants were recruited from seven PREDIMED study centers. Both a baseline cross-sectional (n = 4,377 and a prospective assessment (n = 1,637 were performed. Participants with MetS at baseline were excluded from the longitudinal analysis. The median follow-up was 3.9 years. Anthropometric measurements, blood pressure, fasting glucose, lipid profile and WBC counts were assessed at baseline and yearly during the follow-up. Participants were categorized by baseline WBC and its subtype count quartiles. Adjusted logistic regression models were fitted to assess the risk of MetS and its components. RESULTS: Of the 4,377 participants, 62.6% had MetS at baseline. Compared to the participants in the lowest baseline sex-adjusted quartile of WBC counts, those in the upper quartile showed an increased risk of having MetS (OR, 2.47; 95%CI, 2.03-2.99; P-trend<0.001. This association was also observed for all WBC subtypes, except for basophils. Compared to participants in the lowest quartile, those in the top quartile of leukocyte, neutrophil and lymphocyte count had an increased risk of MetS incidence. Leukocyte and neutrophil count were found to be strongly associated with the MetS components hypertriglyceridemia and low HDL-cholesterol. Likewise, lymphocyte counts were found to be associated with the incidence of the MetS components low HDL-cholesterol and high fasting glucose. An increase in the total WBC during the follow-up was also associated with an increased risk of MetS. CONCLUSIONS: Total WBC counts, and some subtypes

  17. Red Blood Cell Distribution Width and the Platelet Count in Iron-deficient Children Aged 0.5-3 Years.

    Science.gov (United States)

    Akkermans, M D; Uijterschout, L; Vloemans, J; Teunisse, P P; Hudig, F; Bubbers, S; Verbruggen, S; Veldhorst, M; de Leeuw, T G; van Goudoever, J B; Brus, F

    2015-01-01

    Early detection of iron deficiency (ID) and iron deficiency anemia (IDA) in young children is important to prevent impaired neurodevelopment. Unfortunately, many biomarkers of ID are influenced by infection, thus limiting their usefulness. The aim of this study was to investigate the value of red blood cell distribution width (RDW) and the platelet count for detecting ID(A) among otherwise healthy children. A multicenter prospective observational study was conducted in the Netherlands to investigate the prevalence of ID(A) in 400 healthy children aged 0.5-3 years. ID was defined as serum ferritin (SF) count were determined in the complete blood cell count. RDW was inversely correlated with SF and not associated with CRP. Calculated cutoff values for RDW to detect ID and IDA gave a relatively low sensitivity (53.1% and 57.1%, respectively) and specificity (64.7% and 69.9%, respectively). Anemic children with a RDW >14.3% had a 2.7 higher odds (95% confidence interval [CI]: 1.2-6.3) to be iron deficient, compared with anemic children with a RDW count showed a large range in both ID and non-ID children. In conclusion, RDW can be helpful for identifying ID as the cause of anemia in 0.5- to 3-year-old children, but not as primary biomarker of ID(A). RDW values are not influenced by the presence of infection. There appears to be no role for the platelet count in diagnosing ID(A) in this group of children.

  18. Association Between White Blood Cell Count Following Radiation Therapy With Radiation Pneumonitis in Non-Small Cell Lung Cancer

    Energy Technology Data Exchange (ETDEWEB)

    Tang, Chad; Gomez, Daniel R. [Department of Radiation Oncology, The University of Texas MD Anderson Cancer Center, Houston, Texas (United States); Wang, Hongmei [Department of Radiation Oncology, The University of Texas MD Anderson Cancer Center, Houston, Texas (United States); Department of Radiation Oncology, Nanfang Hospital, Southern Medical University, Guangzhou (China); Levy, Lawrence B. [Department of Radiation Oncology, The University of Texas MD Anderson Cancer Center, Houston, Texas (United States); Zhuang, Yan [Department of Radiation Oncology, The University of Texas MD Anderson Cancer Center, Houston, Texas (United States); Department of Radiation Physics, The University of Texas MD Anderson Cancer Center, Houston, Texas (United States); Xu, Ting; Nguyen, Quynh; Komaki, Ritsuko [Department of Radiation Oncology, The University of Texas MD Anderson Cancer Center, Houston, Texas (United States); Liao, Zhongxing, E-mail: zliao@mdanderson.org [Department of Radiation Oncology, The University of Texas MD Anderson Cancer Center, Houston, Texas (United States)

    2014-02-01

    Purpose: Radiation pneumonitis (RP) is an inflammatory response to radiation therapy (RT). We assessed the association between RP and white blood cell (WBC) count, an established metric of systemic inflammation, after RT for non-small cell lung cancer. Methods and Materials: We retrospectively analyzed 366 patients with non-small cell lung cancer who received ≥60 Gy as definitive therapy. The primary endpoint was whether WBC count after RT (defined as 2 weeks through 3 months after RT completion) was associated with grade ≥3 or grade ≥2 RP. Median lung volume receiving ≥20 Gy (V{sub 20}) was 31%, and post-RT WBC counts ranged from 1.7 to 21.2 × 10{sup 3} WBCs/μL. Odds ratios (ORs) associating clinical variables and post-RT WBC counts with RP were calculated via logistic regression. A recursive-partitioning algorithm was used to define optimal post-RT WBC count cut points. Results: Post-RT WBC counts were significantly higher in patients with grade ≥3 RP than without (P<.05). Optimal cut points for post-RT WBC count were found to be 7.4 and 8.0 × 10{sup 3}/μL for grade ≥3 and ≥2 RP, respectively. Univariate analysis revealed significant associations between post-RT WBC count and grade ≥3 (n=46, OR=2.6, 95% confidence interval [CI] 1.4‒4.9, P=.003) and grade ≥2 RP (n=164, OR=2.0, 95% CI 1.2‒3.4, P=.01). This association held in a stepwise multivariate regression. Of note, V{sub 20} was found to be significantly associated with grade ≥2 RP (OR=2.2, 95% CI 1.2‒3.4, P=.01) and trended toward significance for grade ≥3 RP (OR=1.9, 95% CI 1.0-3.5, P=.06). Conclusions: Post-RT WBC counts were significantly and independently associated with RP and have potential utility as a diagnostic or predictive marker for this toxicity.

  19. On-Orbit, Immuno-Based, Label-Free White Blood Cell Counting System with Microelectromechanical Sensor Technology (OILWBCS-MEMS)

    Science.gov (United States)

    Edmonds, Jessica

    2015-01-01

    Aurora Flight Sciences, in partnership with Draper Laboratory, has developed a miniaturized system to count white blood cells in microgravity environments. The system uses MEMS technology to simultaneously count total white blood cells, the five white blood cell differential subgroups, and various lymphocyte subtypes. The OILWBCS-MEMS detection technology works by immobilizing an array of white blood cell-specific antibodies on small, gold-coated membranes. When blood flows across the membranes, specific cells' surface protein antigens bind to their corresponding antibodies. This binding can be measured and correlated to cell counts. In Phase I, the partners demonstrated surface chemistry sensitivity and specificity for total white blood cells and two lymphocyte subtypes. In Phase II, a functional prototype demonstrated end-to-end operation. This rugged, miniaturized device requires minimal blood sample preparation and will be useful for both space flight and terrestrial applications.

  20. Dynamics of erythrocyte count, hemoglobin, and catalase activity in rat blood in hypokinesia, muscular activity and restoration

    Science.gov (United States)

    Taneyeva, G. V.; Potapovich, G. M.; Voloshko, N. A.; Uteshev, A. B.

    1980-01-01

    Tests were conducted to prove that muscular exertion (in this instance swimming) of different duration and intensity, as well as hypodynamia, result in an increase of hemoglobin and number of red blood cells in peripheral blood rats. Catalase activity increased with an increase in the duration of swimming, but only up to 6 hr; with 7-9 hr of swimming as well as in hypodynamia, catalase activity decreased. It was also observed that under hypodynamia as well as in 3, 5 and 6 hr exertion (swimming) the color index of blood decreased. Pressure chamber treatment (for 8 min each day for one week), alternating a 2 min negative pressure up to 35 mm Hg with 1 min positive pressure, increased the erythrocyte count and hemoglobin content.

  1. Seasonal Variations of Complete Blood Count and Inflammatory Biomarkers in the US Population - Analysis of NHANES Data.

    Directory of Open Access Journals (Sweden)

    Bian Liu

    Full Text Available Recent studies reported seasonal differences in gene expression in white blood cells, adipose tissue, and inflammatory biomarkers of the immune system. There is no data on the seasonal variations of these biomarkers in the US general population of both children and adults. Then aim of this study is to explore the seasonal trends in complete blood count (CBC, and C-reactive protein (CRP in a large non-institutionalized US population.Seven cross-sectional data collected in the National Health and Nutrition Examination Survey (NHANES during 1999-2012 were aggregated; participants reporting recent use of prescribed steroids, chemotherapy, immunomodulators and antibiotics were excluded. Linear regression models were used to compare levels of CBC and CRP between winter-spring (November-April and summer-fall (May-October, adjusting for demographics, personal behavioral factors, and chronic disease conditions.A total of 27,478 children and 36,644 adults (≥18 years were included in the study. Levels of neutrophils, white blood cell count (WBC, and CRP were higher in winter-spring than summer-fall (p≤0.05. Red blood cell components were lower in winter-spring than in summer-fall, while the opposite was seen for platelets.This large population-based study found notable seasonal variations in blood cell composition and inflammatory biomarkers, with a more pro-inflammatory immune system seen in winter-spring than summer-fall. The red blood cell patterns could have implications for the observed cardio-vascular seasonality.

  2. High oxygen modifies vasodilator effect of cysteine via enhanced oxidative stress and thromboxane production in the rat mesenteric artery.

    Science.gov (United States)

    Yasuda, Yoshitaka; Feng, Guo-Gang; Li, Jiazheng; Nakamura, Emi; Hayashi, Hisaki; Sato, Motohiko; Fujiwara, Yoshihiro; Kinoshita, Hiroyuki

    2016-09-01

    Whether high oxygen is harmful to the vascular function is unclear. The present study examined if high oxygen modifies vasodilator effect of cysteine via enhanced oxidative stress and thromboxane production. Rat mesenteric arteries with endothelium at 95 or 50 % oxygen were subjected to isometric force recordings, measurement of thromboxane B2 levels, determination of superoxide and peroxynitrite levels and evaluation of NADPH oxidase subunit protein expression, respectively. L-cysteine (0.01-3 mM) constricted or dilated arteries at 95 and 50 % oxygen, respectively. Thromboxane receptor antagonist SQ-29,548 (1 μM) abolished the constriction at 95 % oxygen. L-cysteine (3 mM) increased levels of thromboxane B2 in arteries upon 95 % oxygen application. L-cysteine relaxed arteries treated with superoxide inhibitor tiron (2 mM) or NADPH oxidase inhibitor gp91ds-tat (1 μM) irrespective of the oxygen concentration while ATP-sensitive K(+) channel inhibitor glibenclamide (1 μM) and cystathionine-γ-lyase (CSE) inhibitor DL-propargylglycine (10 mM) similarly abolished the relaxation. L-cysteine (3 mM) with 95 % oxygen augmented levels of superoxide as well as nitrotyrosine within the artery, concomitantly with enhanced membrane protein expression of NADPH oxidase subunit p47phox. The higher concentration of oxygen attenuates L-cysteine-induced vasodilation via superoxide production mediated by NADPH oxidase along with thromboxane A2 production, resulting in vasoconstriction. The increased levels of superoxide, as well as peroxynitrite, coexist with the impaired vasodilation related to ATP-sensitive K(+) channels and CSE. Higher oxygen with plasma cysteine may cause oxidative stress and vasoconstrictor prostanoid production in blood vessels.

  3. Decreased blood platelet volume and count in patients with liver disease

    DEFF Research Database (Denmark)

    Jørgensen, B; Fischer, E; Ingeberg, S;

    1984-01-01

    Mean platelet volume (MPV) and count (PLT) were assessed in patients with moderately affected liver function. PLT was significantly decreased in patients with liver disease (197 X 10(9)l-1 +/- 87 (SD), no. = 79) compared with that of controls (273 X 10(9)l-1 +/- 53 (SD), no. = 37, P less than 0...

  4. Smart fast blood counting of trace volumes of body fluids from various mammalian species using a compact custom-built microscope cytometer (Conference Presentation)

    Science.gov (United States)

    Smith, Zachary J.; Gao, Tingjuan; Lin, Tzu-Yin; Carrade-Holt, Danielle; Lane, Stephen M.; Matthews, Dennis L.; Dwyre, Denis M.; Wachsmann-Hogiu, Sebastian

    2016-03-01

    Cell counting in human body fluids such as blood, urine, and CSF is a critical step in the diagnostic process for many diseases. Current automated methods for cell counting are based on flow cytometry systems. However, these automated methods are bulky, costly, require significant user expertise, and are not well suited to counting cells in fluids other than blood. Therefore, their use is limited to large central laboratories that process enough volume of blood to recoup the significant capital investment these instruments require. We present in this talk a combination of a (1) low-cost microscope system, (2) simple sample preparation method, and (3) fully automated analysis designed for providing cell counts in blood and body fluids. We show results on both humans and companion and farm animals, showing that accurate red cell, white cell, and platelet counts, as well as hemoglobin concentration, can be accurately obtained in blood, as well as a 3-part white cell differential in human samples. We can also accurately count red and white cells in body fluids with a limit of detection ~3 orders of magnitude smaller than current automated instruments. This method uses less than 1 microliter of blood, and less than 5 microliters of body fluids to make its measurements, making it highly compatible with finger-stick style collections, as well as appropriate for small animals such as laboratory mice where larger volume blood collections are dangerous to the animal's health.

  5. Leukocyte count affects expression of reference genes in canine whole blood samples

    NARCIS (Netherlands)

    Piek, C.J.; Brinkhof, B.; Rothuizen, J.; Dekker, A.; Penning, L.C.

    2011-01-01

    Background The dog is frequently used as a model for hematologic human diseases. In this study the suitability of nine potential reference genes for quantitative RT-PCR studies in canine whole blood was investigated. Findings The expression of these genes was measured in whole blood samples of 263 i

  6. ZINC SERUM LEVEL AND PERIPHERAL BLOOD MONOCYTE COUNT OF MULTIBACILAR LEPROSY PATIENT LOWER THAN PAUCIBACILAR LEPROSY IN RSUP SANGLAH

    Directory of Open Access Journals (Sweden)

    Putu Kurniawan Dhana

    2013-04-01

    Full Text Available Zinc has been known to have important role in the immune system. Zinc deficiency can inhibit activation and production cytokine of Th1 and  may cause cellular immunity dysfunction. This conditon also may cause changes of lymphopoiesis and hematopoiesis also peripheral blood of mononuclear cell as mononuclear fagocyte. The Aim of this study is to know zinc serum status and peripheral blood monocyte count of leprosy patient in Dermato Venerologi policlinic Sanglah hospital Denpasar. This study use cross sectional design. Sample of study take by consecutive sampling with sample size contains 75 patient.  Mean of zinc serum status on multibacillary leprosy patient is 5.66  (SB 11.74 found lower compare to paucibacillary leprosy patient 19.38 (SB 18.21 and statistically significant with P < 0.05. Mean of peripheral blood monocyte count in multibacillary patient is 7.12 (SB 2.53 lower compare to paucibacillary leprosy patient with 7.88 (SB 3.08, but statistically not significant with P > 0.05. Binary logistic analysis show the influence of zinc serum status to probability to have leprosy. This study suggest correction of serum zinc level in leprosy patient through nutritional approach or the granting of a supplement of zinc

  7. Selected complete blood cell count and plasma protein electrophoresis parameters in pet psittacine birds evaluated for illness.

    Science.gov (United States)

    Briscoe, Jeleen A; Rosenthal, Karen L; Shofer, Frances S

    2010-06-01

    Veterinarians rely on results of both the complete blood cell count (CBC) and plasma protein electrophoresis (EPH) in conjunction with the results of the plasma biochemical analysis to evaluate the health status of avian patients. Because the CBC and protein EPH measure different aspects of the immune response to disease, both tests are recommended in avian patients to rule out infectious or inflammatory disease. To evaluate results of the CBC and protein EPH in pet psittacine birds, the records of 144 pet psittacine birds, comprising 11 genera, that were presented for suspected illness were reviewed. Results of the CBC (total white blood cell count and packed cell volume) and protein EPH (alpha, beta, and gamma globulin concentrations) from submitted blood samples from each bird were evaluated. Of the 144 birds, 63 (43.8%) had abnormal CBC results, and 25 (17.4%) had abnormal EPH measurements. Results of the CBC and protein EPH were within reference ranges in 73 birds (50.7%). Abnormal results of the CBC in conjunction with normal EPH results were present in 46 birds (31.9%), compared with 8 birds (5.6%) with normal results of the CBC and abnormal EPH results. The findings of this study could aid practitioners in evaluating psittacine patients and prioritizing the value of individual diagnostic tests.

  8. On Orbit Immuno-Based, Label-Free, White Blood Cell Counting System with MicroElectroMechanical Sensor (MEMS) Technology (OILWBCS-MEMS) Project

    Data.gov (United States)

    National Aeronautics and Space Administration — Aurora Flight Sciences Corporation and partner, Draper Laboratory, propose to develop an on-orbit immuno-based label-free white blood cell counting system using MEMS...

  9. On Orbit Immuno-Based, Label-Free, White Blood Cell Counting System with MicroElectroMechanical Sensor (MEMS) Technology (OILWBCS-MEMS) Project

    Data.gov (United States)

    National Aeronautics and Space Administration — Aurora Flight Sciences Corporation and our partner, Draper Laboratory, propose to develop an on orbit immuno-based, label-free, white blood cell counting system for...

  10. Evaluation of correlation between nucleated red blood cell count in term newborns and meconium-stained amniotic fluid

    Directory of Open Access Journals (Sweden)

    Esmaeilian L

    2001-09-01

    Full Text Available Considering the incidence of meconium-stained amniotic fluid (MSAF in newborns and its complications, and also based on indirect reports pointing out relationship between nucleated red blood cell (nRBC, as a marker of chronic hypoxia, and MSAF in term newborns, and in order to determine this relationship more accurately, this study was done on women with uncomplicated singleton term pregnancy that admitted for delivery at Shariati hospital in year 2000. After excluding confounding factors on nRBC, case group who includes infants with MSAF, and control group who have clear amniotic fluid were determined. Maternal age and parity, gestational age, birth weight, apgar score, neonatal out come, and FHR pattern as well as newborn hemoglobin values were evaluated. Venous cord blood was analyzed for nRBC counts per 100 WBC. Case and control groups were divided according to nRBC counts with cutoff point of 10 and then compared statistically. From the samples, 117 cases and 67 controls were evaluated. Maternal and neonatal factors were similar in two situations. Number of nRBC was 3.75±4.8 in control group and 12.04±11.7 in case group (P<0.01 and it was abnormal (>10 in 9 percent of newborns with clear fluid and 32 percent of MSAF (P<0.01. Cord blood nRBC count increased when meconium is passed intrauterine. This suggests that cases with MSAF may be at risk of chronic hypoxia. Cohort research is recommended to study the affect of delivery mode on the neonatal outcome in cases with MSAF and to evaluate the cause of fetal hypoxia in uncomplicated pregnancy with MSAF.

  11. Reference intervals of complete blood count constituents are highly correlated to waist circumference: should obese patients have their own "normal values?".

    Science.gov (United States)

    Vuong, Jennifer; Qiu, Yuelin; La, Myanh; Clarke, Gwen; Swinkels, Dorine W; Cembrowski, George

    2014-07-01

    Body mass index (BMI), the prevalent indicator of obesity, is not easily grasped by patients nor physicians. Waist circumference (WC) is correlated to obesity, is better understood and has a stronger relationship to the metabolic syndrome. We compiled WC, complete blood count (CBC) parameters as well as other pertinent data of 6766 25-55-year-old US volunteers sampled in the US National Health and Nutrition Examination Survey, in the years 2005-2010. To determine reference intervals of typical US patients visiting their clinician, we used minimal exclusion criteria. We compiled hemoglobin, red blood cell count, hematocrit, mean corpuscular volume (MCV), mean corpuscular hemoglobin concentration, mean cell hemoglobin (MCH), red cell distribution width (RDW), platelet count, mean platelet volume, and counts of white blood cells (WBC), neutrophils, lymphocytes, monocytes, eosinophils, and basophils. In addition, we also compiled serum C reactive protein and serum iron. The three major US races were studied and reference interval diagrams were constructed for each CBC parameter plotted against WC. WBC count, RDW, lymphocyte, neutrophil, and red blood cell count increase with WC. Conversely, serum iron and MCH and MCV decrease. These relationships may be related to insulin resistance and chronic activation of the immune system and the resulting low-grade inflammatory state. WC is a strong predictor for many CBC parameters, suggesting that WC should be taken into account when evaluating blood count results. Clinicians who take care of obese patients should be aware of altered hematology and investigate and treat accordingly.

  12. [Accreditation of automated complete blood count by the LH750 Analyzer (Beckman Coulter) in Georges Pompidou Hospital (Paris, France)].

    Science.gov (United States)

    Robinet, Sylvain; Lemaire, Pierre; Louis, Gauthier; Vieillefond, Vincent; Daigneau, Yolande; Gaillaud, Emilie; Vincent, Béatrice; Fischer, Anne-Marie; Siguret, Virginie

    2013-01-01

    Preliminary evaluation of quantitative clinical laboratory measurements is a prerequisite for the accreditation of clinical laboratories, according to the French Committee of Accreditation guidelines following the European reference Standard EN ISO 15189. Numerous papers have been published regarding biochemistry and immunology. However, data are lacking for automated complete blood count accreditation. We report here our experience at Hôpital européen Georges Pompidou hematology laboratory and present the performance characteristics of two mirrored LH750 Beckman-Coulter analysers, including precision, accuracy and uncertainty of measurement.

  13. An Experimental Study on RBC Count and Serum Potassium Concentration Changes During Compres-sion Transfusion of WBC-removal Whole Blood

    Institute of Scientific and Technical Information of China (English)

    Jin-Lian Cheng; Shi-Fan Han; Ya-Qin Li; Yin-Ping Chu; Yu-Mei Sun; Jin-Feng Guo

    2015-01-01

    Objective: To observe changes in RBC count, changes, RBC morphology, and serum potassium during compressed transfusion of WBC-removal whole blood. Methods: Prepared human WBC-removal whole blood and connected transfusion apparatus with different sizes of intravenous detaining needles ( 18G, 20G, 22G and 24G) . Observed RBC count and serum potassium concentration under different pressures ( 100 mmHg, 200 mmHg, and 300 mmHg) as blood flowed out of the pinhead end of the intravenous detaining needle. Samples ob-tained with the 20G needle were smeared on glass slides, and RBC morphologic changes were ob-served under an oil immersion lens. Results: RBC count and serum potassium changed slightly under different pressures with differ-ent sizes of intravenous detaining needles as blood flowed through the transfusion apparatus. In ad-dition, the observation of blood samples under a common light microscope revealed that coarse-prick, oblong, and spindle cell counts in the visual fields increased gradually as the pressure in-creased. Additionally, a portion of cells had undergone splintering. Conclusions: While applying 18G, 20G, 22G and 24G intravenous detaining needles for blood transfusion under less than 300 mmHg of pressure, no significant RBC count change was found in blood samples in the short term. However, there were significant RBC morphologic changes. The results could offer more basis to ensure the clinical safety of patients undergoing blood transfusion.

  14. Smoking, white blood cell counts, and TNF system activity in Japanese male subjects with normal glucose tolerance

    Directory of Open Access Journals (Sweden)

    Watanabe Naoya

    2011-11-01

    Full Text Available Abstract Background Cigarette smokers have increased white blood cell (WBC counts and the activation of tumor necrosis factor (TNF. The effect of smoking on WBC counts and TNF system activity, however, has not been separately investigated yet. Subjects and Methods One hundred and forty-two Japanese male subjects with normal glucose tolerance were recruited. They were stratified into two groups based on the questionnaire for smoking: one with current smokers (n = 48 and the other with current non-smokers (n = 94. Whereas no significant differences were observed in age, BMI, high molecular weight (HMW adiponectin, and TNF-α between the two groups, current smokers had significantly higher soluble TNF receptor 1 (sTNF-R1 (1203 ± 30 vs. 1116 ± 21 pg/ml, p = 0.010 and increased WBC counts (7165 ± 242 vs. 5590 ± 163/μl, p p = 0.031 as compared to current non-smokers. Next, we classified 48 current smokers into two subpopulations: one with heavy smoking (Brinkman index ≥ 600 and the other with light smoking (Brinkman index Results Whereas no significant difference was observed in age, BMI, HMW adiponectin, WBC counts and TNF-α, sTNF-R1 and sTNF-R2 were significantly higher in heavy smoking group (1307 ± 44 vs. 1099 ± 30 pg/ml, p p = 0.005 than in light smoking group, whose sTNF-R1 and sTNF-R2 were similar to non-smokers (sTNF-R1: 1116 ± 15 pg/ml, p = 0.718, sTNF-R2; 1901 ± 32 pg/ml, p = 0.437. In contrast, WBC counts were significantly increased in heavy (7500 ± 324/μl, p p = 0.001 smoking group as compared to non-smokers (5590 ± 178/μl. There was no significant difference in WBC counts between heavy and light smoking group (p = 0.158. Conclusion We can hypothesize that light smoking is associated with an increase in WBC counts, while heavy smoking is responsible for TNF activation in Japanese male subjects with normal glucose tolerance.

  15. Absolute counts of peripheral blood leukocyte subpopulations in intraabdominal sepsis and pneumonia-derived sepsis: a pilot study Absolute counts of peripheral blood leukocyte subpopulations in intraabdominal sepsis and pneumonia-derived sepsis: a pilot study

    Directory of Open Access Journals (Sweden)

    Grazyna Anna Hoser

    2012-10-01

    Full Text Available The leading pathophysiological changes during sepsis include systemic abnormalities in the immune
    response. Due to the general character of these disturbances, sepsis is usually studied as a homogenous clinical
    condition. We aimed to compare the immune response in intraabdominal sepsis (IAS and pneumonia-derived
    sepsis (PDS. The following cell populations were examined: white blood cell count (WBC, monocytes, lymphocytes:
    CD3+, CD4+ and CD8+ T cells, B cells, and NK cells. In both studied groups (i.e. IAS and PDS, the
    WBC was elevated. However, it was significantly higher in the IAS group than in the PDS group. The difference
    was due to a lower granulocyte count, as well as a lower monocyte count in PDS. We found no significant
    correlation between the total lymphocyte number and CD3+CD8+ T cells in either form of sepsis. Similarly, we
    observed no correlation between the total lymphocyte number and the NK cells subset in IAS. However, the
    numbers of CD3+CD8+ and NK cells correlated similarly in both types of sepsis. Both studied types of sepsis
    induced profound lymphocytopenia, with marked loss of CD8+ T cells and the NK cells. However, the similar
    relation between them, which was independent of the infection type, suggests that the NK and CD3+CD8+ cells
    have shared mechanisms of regulation. The primary site of infection has an impact on the global immune reaction.
    These alternations include especially myeloid cells: granulocytes and monocytes which disappear from peripheral
    blood during PDS, but increase in IAS.
    The leading pathophysiological changes during sepsis include systemic abnormalities in the immune
    response. Due to the general character of these disturbances, sepsis is usually studied as a homogenous clinical
    condition. We aimed to compare the immune response in intraabdominal sepsis (IAS and pneumonia-derived
    sepsis (PDS. The following cell

  16. Identification of RBC and WBC Count in Human Blood Using ARM Based Instrumentation

    Directory of Open Access Journals (Sweden)

    B.Dodda Basavanagoud

    2015-06-01

    Full Text Available The rapid growth in microelectronics and crunching RISC in the field of bio-medical sciences incorporated of soft tools to diagnose various parameters of human fluids. Conventional method of blood sample analysis makes use of laboratory technique of titration, which is operator-dependent and results in lot of errors depending on the skill of the technician. In order to eliminate the human errors involved in the conventional method, in this paper an attempt has been made to present a capillary centrifuge technique driven by high speed DC motor fed by Morgan chopper and controlled by powerful ARM processor. It results in accurate analysis of the blood samples. The various techniques involved in accurate sensing of speed using timer and generation of firing pulses to thyristor in the Morgan chopper is judiciously achieved. This paper clearly brings out the advantages of the proposed blood measurement technique which effectively gives blood analysis faster and at a low cost.

  17. C-reactive protein and white blood cell count do not improve clinical decision-making in acute appendicitis

    DEFF Research Database (Denmark)

    Tind, Sofie; Lassen, Annmarie Touborg; Zimmermann-Nielsen, Erik;

    2015-01-01

    INTRODUCTION: Acute appendicitis (AA) remains a diagnostic challenge as indicated by the high rate of unnecessary surgery. Blood samples, primarily C-reactive protein (CRP) and leucocyte counts, are used as a diagnostic supplement despite their relatively low sensitivities and specificities....... However, their influence on diagnostic decision-making has not previously been investigated. The aim of the present study was to investigate if the results of CRP and leucocytes had any positive or negative influence on the decision-making of surgeons handling patients with suspected AA. METHODS...... the blood results and re-evaluate their diagnosis. The surgeon's diagnosis before and after was compared with the final diagnosis defined by surgical findings or follow-up. The gold standard was any degree of appendicitis on histology. RESULTS: A total of 226 patients were included of whom 91 (40.3%) had...

  18. Interleukin-2 dose, blood monocyte and CD25+ lymphocyte counts as predictors of clinical response to interleukin-2 therapy in patients with renal cell carcinoma

    DEFF Research Database (Denmark)

    Hermann, G G; Geertsen, P F; von der Maase, H;

    1991-01-01

    schedule with continuous intravenous IL-2 infusion (3 x 10(6) units m-2 day-1) administered from days 1 to 5 and days 12 to 16. The white blood cell count and the absolute and relative number of neutrophils, lymphocytes, eosinophils and monocytes were recorded six times in peripheral blood during...... the treatment. Also the blood counts of T cell and NK cell subsets and cells expressing the T cell activation markers IL-2R alpha and VLA-1 were measured. The lymphokine-activated killer (LAK) cell cytotoxicity was measured either with or without additional in vitro stimulation by IL-2. Multivariate statistical...... analysis showed that the clinical responses were related to the administered dose of IL-2, to a low number of blood cells expressing IL-2 receptors and to a reduction in the blood monocyte count (P less than 0.05)....

  19. Expression of CD56 is an unfavorable prognostic factor for acute promyelocytic leukemia with higher initial white blood cell counts.

    Science.gov (United States)

    Ono, Takaaki; Takeshita, Akihiro; Kishimoto, Yuji; Kiyoi, Hitoshi; Okada, Masaya; Yamauchi, Takahiro; Emi, Nobuhiko; Horikawa, Kentaro; Matsuda, Mitsuhiro; Shinagawa, Katsuji; Monma, Fumihiko; Ohtake, Shigeki; Nakaseko, Chiaki; Takahashi, Masatomo; Kimura, Yukihiko; Iwanaga, Masako; Asou, Norio; Naoe, Tomoki

    2014-01-01

    Expression of CD56 has recently been introduced as one of the adverse prognostic factors in acute promyelocytic leukemia (APL). However, the clinical significance of CD56 antigen in APL has not been well elucidated. We assessed the clinical significance of CD56 antigen in 239 APL patients prospectively treated with all-trans retinoic acid and chemotherapy according to the Japan Adult Leukemia Study Group APL97 protocol. All patients were prospectively treated by the Japan Adult Leukemia Study Group APL97 protocol. The median follow-up period was 8.5 years. Positive CD56 expression was found in 23 APL patients (9.6%). Expression of CD56 was significantly associated with lower platelet count (P = 0.04), severe disseminated intravascular coagulation (P = 0.04), and coexpression of CD2 (P = 0.03), CD7 (P = 0.04), CD34 (P < 0.01) and/or human leukocyte antigen-DR (P < 0.01). Complete remission rate and overall survival were not different between the two groups. However, cumulative incidence of relapse and event-free survival (EFS) showed an inferior trend in CD56(+) APL (P = 0.08 and P = 0.08, respectively). Among patients with initial white blood cell counts of 3.0 × 10(9)/L or more, EFS and cumulative incidence of relapse in CD56(+) APL were significantly worse (30.8% vs 63.6%, P = 0.008, and 53.8% vs 28.9%, P = 0.03, respectively), and in multivariate analysis, CD56 expression was an unfavorable prognostic factor for EFS (P = 0.04). In conclusion, for APL with higher initial white blood cell counts, CD56 expression should be regarded as an unfavorable prognostic factor.

  20. Evaluation of total IgE, CRP and blood count parameters in children with asthma and allergic rhinitis

    Directory of Open Access Journals (Sweden)

    Feti Tülübaş

    2013-03-01

    Full Text Available Objective: This study aimed to research retrospectivelywhether asthma and allergic rhinitis are related to totalIgE, C-reactive protein (CRP and complete blood countparameters.Materials and methods: Files of 443 children who appliedto pediatric outpatient clinics of our hospital,aged2-18 were retrospectively investigated. Patients weregrouped into three as asthma (n=179, allergic rhinitis (n=171 and control group (n= 93. Patients’ ages, genders,total IgE, CRP and hemogram values were recorded.Results: While eosinophil count, MCHC and total IgElevels were significantly higher in asthma group, MCVlevels were significantly lower. Lymphocyte count, CRPand total IgE levels were significantly higher in allergicrhinitis group compared with control group whereas neutrophilcount were significantly lower and eosinophil countdid not change significantly. Total IgE levels were higherin asthma and allergic rhinitis compared with controls.However, CRP levels were higher only in allergic rhinitisgroup. MCV levels were significantly lower in asthmagroup compared with controls. MCHC levels were significantlyhigher in asthma group compared with allergicrhinitis and control groups. Neutrophil count decreasedwhile lymphocyte count increased significantly. Eosinophilcount significantly increased compared with controlgroup whereas a significant difference was not observedbetween allergic rhinitis and controls.Conclusions: Our findings suggest factors effective inasthma pathogenesis might be effective also in erythrocytemorphology. There are remarkable changes in bloodeosinophil levels in asthma and in neuthrophil and lymphocytelevels in allergic rhinitis. Serum total IgE level increasesin asthma group whereas it decreases in allergicrhinitis group.Key words: Asthma, allergic rhinitis, total IgE, CRP, MCV

  1. Influence of the first radioactive iodine ablation on peripheral complete blood count in patients with differentiated thyroid cancer

    Science.gov (United States)

    Hu, Tianpeng; Meng, Zhaowei; Zhang, Guizhi; Jia, Qiang; Tan, Jian; Zheng, Wei; Wang, Renfei; Li, Xue; Liu, Na; Zhou, Pingping; Upadhyaya, Arun

    2016-01-01

    Abstract Radioactive iodine (RAI) is considered to be related with hematologic changes. This study aimed to evaluate influence of the first RAI ablation on peripheral complete blood count (CBC) in patients with differentiated thyroid cancer (DTC). Data of CBC at baseline and 6 months after RAI were obtained in 385 patients with DTC with approximately 3700 MBq 131I (ranging 2220–7585 MBq). Further comparison was done in 196 patients with 1-month postablation data available. Routine blood examinations were performed to determine impact of RAI on white blood cell (WBC), red blood cell (RBC), hemoglobin, platelet, neutrophil, lymphocyte, and monocyte in both sexes. Continuous variables were compared by paired t tests and independent samples t test, and categorical variables were compared by chi-square analysis. Data with repeated measurements were analyzed by analysis of variance. The first RAI after thyroidectomy was associated with mild, yet significant declines in WBC, platelet, and lymphocyte, which persisted for 6 months. One month after RAI, significant declines were found in all CBC, including RBC and hemoglobin (all P DTC. PMID:27583853

  2. Four-Parameter white blood cell differential counting based on light scattering measurements

    NARCIS (Netherlands)

    Terstappen, L.W.M.M.; Grooth, de B.G.; Visscher, K.; Kouterik, F.A.; Greve, J.

    1988-01-01

    Measurement of the depolarized orthogonal light scattering in flow cytometry enables one to discriminate human eosinephilic granulocytes from neutrophilic granulocytes. We use this method to perform a four-parameter differential white blood cell analysis. A simple flow cytometer was built equipped

  3. The Influence of Low Platelet Count on Whole Blood Aggregometry Assessed by Multiplate

    DEFF Research Database (Denmark)

    Stissing, Trine; Dridi, Nadia P; Ostrowski, Sisse R

    2011-01-01

    in an artificial matrix, platelet-rich plasma (PRP). Heparinized and citrated blood was diluted with autologous plasma to platelet concentrations 200 to 25 × 10(9)/L in WB samples (n = 10) and 200 to 100 × 10(9)/L in PRP samples (n = 7). The platelet aggregation was investigated by the ADP-, ASPI-, COL-, and TRAP...

  4. Differential associations between white blood cell counts and fatigue in young and older adults

    DEFF Research Database (Denmark)

    Avlund, Kirsten; Hokland, Marianne; Mehlsen, Mimi Y;

    2012-01-01

    The aims of this exploratory study were to study whether fatigue might be related to the cellular immune system by 1) analysing if the number of white blood cell subsets are related to fatigue and 2) if possible relationships vary in younger and older community-dwelling individuals....

  5. High white blood cell count at diagnosis of childhood acute lymphoblastic leukaemia: biological background and prognostic impact. Results from the NOPHO ALL-92 and ALL-2000 studies

    DEFF Research Database (Denmark)

    Vaitkeviciene, G; Forestier, E; Hellebostad, M;

    2011-01-01

    Prognostic impact of peripheral blood white blood cell count (WBC) at the diagnosis of childhood acute lymphoblastic leukaemia (ALL) was evaluated in a population-based consecutive series of 2666 children aged 1–15 treated for ALL between 1992 and 2008 in the five Nordic countries (Denmark, Finland...

  6. Determination of micro-litre volumes with high accuracy for flow cytometric blood cell counting

    Science.gov (United States)

    Reitz, S.; Kummrow, A.; Kammel, M.; Neukammer, J.

    2010-07-01

    We have gravimetrically calibrated the volumes dispensed by 1 mL syringes in the range between 1 µL and 100 µL using ultra-pure water. Protocols are based on series of consecutive difference measurements of masses in order to precisely compensate for evaporation, being the most important disturbing quantity. We determined expanded uncertainties of volume measurements for glass syringes of typically 0.2% (expansion factor 2) when dispensing volumes of 10 µL. For polypropylene syringes, selected with respect to the manufacturer, expanded uncertainties of 0.25% (expansion factor 2) were observed. Calibrated syringes were applied for measuring concentrations of blood cells in a flow cytometer demonstrating the capability to determine reference measurement values. Since the direct interaction of blood cells and syringe walls may lead to cell adhesion, glass syringes as well as (disposable) polypropylene syringes were calibrated.

  7. The Peripheral Blood Mononuclear Cell Count Is Associated With Bone Health in Elderly Men: A Cross-Sectional Population-Based Study.

    Science.gov (United States)

    Lin, Xianfeng; Yu, Hejun; Zhao, Chenchen; Qian, Yu; Hong, Dun; Huang, Kangmao; Mo, Jian; Qin, An; Fang, Xiangqian; Fan, Shunwu

    2016-04-01

    The peripheral blood mononuclear cell (PBMC) count is a routinely used and meaningful index for infection and blood diseases. PBMCs may be closely related to osteoclasts and include osteoclast precursors; we examined the association between the PBMC count and bone health. This research included 2806 community men aged ≥50 years who underwent full health examinations from October 2007 through December 2011 in four medical centers. The PBMC count was significantly high among subjects with "at least osteopenia" compared with controls. In analysis of covariance adjusted for potential confounders, the bone mineral density (BMD) value and T-score had a significant decreasing trend across the quartiles of PBMC count. In univariate analysis, the PBMC count had a strong association with "at least osteopenia" (odds ratio [OR] = 2.520, 95% confidence interval [CI]: 1.397-4.547). After adjustment for confounding factors (multivariate analysis) from Model 1 to 4, PBMC count remained as an independent risk factor for "at least osteopenia" (OR = 2.481, 95% CI: 1.176-5.236). Moreover, after adjusting for all confounding variables, participants had a significantly high OR in the body mass index (BMI) blood pressure (SBP) count is significantly associated with bone loss in elderly men and the exact mechanism requires further clarification.

  8. EFFECT OF PARTURITION ON WHITE BLOOD CELLS COUNT (WBC AND T CELLS SUBSETS IN SELENIUM SUPPLEMENTED NEWBORN LAMBS

    Directory of Open Access Journals (Sweden)

    L. PISEK

    2013-12-01

    Full Text Available The aim of the work was assessed the influence of parturition at the dynamics of leukocytes and T cells subsets in a selenium supplemented newborn lambs. The experiment was conducted on nineteen sucking newborn lambs of the Sumava sheep breed. After parturition blood samples were taken from lambs on day 10, 30 and 60. The WBC in blood smear was detected by microscopically analysis (norm no. 84 3206, and the CD4+ and CD8+ T cells subsets in blood were detected by flow cytometery. The WBC was in physiological norm. The highest WBC was founded on 30th day of the experiment. In the dynamics of the CD4+ a CD8+ T cells subsets were founded statistically significant differences: In the CD4+ subset between 10th and 30th day of the experiment (P < 0.001 and between 30th and 60th day of the experiment (P < 0.01, and in the CD8+ subset between 10th and 30th day of the experiment (P < 0.001. Excepted in 30th day of the experiment were counts of the CD4+ and CD8+ T cells subsets inside the physiological norm.

  9. Genetic Modifiers of White Blood Cell Count, Albuminuria and Glomerular Filtration Rate in Children with Sickle Cell Anemia

    Science.gov (United States)

    Flanagan, Jonathan M.; Alvarez, Ofelia A.; Nelson, Stephen C.; Aygun, Banu; Nottage, Kerri A.; George, Alex; Roberts, Carla W.; Piccone, Connie M.; Howard, Thad A.; Davis, Barry R.; Ware, Russell E.

    2016-01-01

    Discovery and validation of genetic variants that influence disease severity in children with sickle cell anemia (SCA) could lead to early identification of high-risk patients, better screening strategies, and intervention with targeted and preventive therapy. We hypothesized that newly identified genetic risk factors for the general African American population could also impact laboratory biomarkers known to contribute to the clinical disease expression of SCA, including variants influencing the white blood cell count and the development of albuminuria and abnormal glomerular filtration rate. We first investigated candidate genetic polymorphisms in well-characterized SCA pediatric cohorts from three prospective NHLBI-supported clinical trials: HUSTLE, SWiTCH, and TWiTCH. We also performed whole exome sequencing to identify novel genetic variants, using both a discovery and a validation cohort. Among candidate genes, DARC rs2814778 polymorphism regulating Duffy antigen expression had a clear influence with significantly increased WBC and neutrophil counts, but did not affect the maximum tolerated dose of hydroxyurea therapy. The APOL1 G1 polymorphism, an identified risk factor for non-diabetic renal disease, was associated with albuminuria. Whole exome sequencing discovered several novel variants that maintained significance in the validation cohorts, including ZFHX4 polymorphisms affecting both the leukocyte and neutrophil counts, as well as AGGF1, CYP4B1, CUBN, TOR2A, PKD1L2, and CD163 variants affecting the glomerular filtration rate. The identification of robust, reliable, and reproducible genetic markers for disease severity in SCA remains elusive, but new genetic variants provide avenues for further validation and investigation. PMID:27711207

  10. Full blood count and haemozoin-containing leukocytes in children with malaria: diagnostic value and association with disease severity

    Directory of Open Access Journals (Sweden)

    Lell Bertrand

    2008-06-01

    Full Text Available Abstract Background Diligent and correct laboratory diagnosis and up-front identification of risk factors for progression to severe disease are the basis for optimal management of malaria. Methods Febrile children presenting to the Medical Research Unit at the Albert Schweitzer Hospital (HAS in Lambaréné, Gabon, were assessed for malaria. Giemsa-stained thick films for qualitative and quantitative diagnosis and enumeration of malaria pigment, or haemozoin (Hz-containing leukocytes (PCL were performed, and full blood counts (FBC were generated with a Cell Dyn 3000® instrument. Results Compared to standard light microscopy of Giemsa-stained thick films, diagnosis by platelet count only, by malaria pigment-containing monocytes (PCM only, or by pigment-containing granulocytes (PCN only yielded sensitivities/specificities of 92%/93%; 96%/96%; and 85%/96%, respectively. The platelet count was significantly lower in children with malaria compared to those without (p ® instrument detected significantly more patients with PCL (p Conclusion In the age group examined in the Lambaréné area, platelets are an excellent adjuvant tool to diagnose malaria. Pigment-containing leukocytes (PCL are more readily detected by automated scatter flow cytometry than by microscopy. Automated Hz detection by an instrument as used here is a reliable diagnostic tool and correlates with disease severity. However, clinical usefulness as a prognostic tool is limited due to an overlap of PCL numbers recorded in severe versus non-severe malaria. However, this is possibly because of the instrument detection algorithm was not geared towards this task, and data lost during processing; and thus adjusting the instrument's algorithm may allow to establish a meaningful cut-off value.

  11. Analysis of feasibility of blood cell count in blood emergency arterial blood gas%急诊动脉血气分析用血进行血细胞计数的可行性探讨

    Institute of Scientific and Technical Information of China (English)

    黄万仁; 张桂芬; 张杰雄

    2014-01-01

    Objective To explore the feasibility of blood cell count in blood emergency arterial blood gas. Methods 153 patients of the emergency department of our hospital in 2008 March to 2013October were collected,arterial blood gas analysis andblood cell count were performed in all patients at the same time were all observed. Results Venous blood and the instant blood blood cell counts were compared,comparison of MCV,MCH,MCHC,MPV, PDW,P-LCR,BASO,the difference was statistically significant(P < 0.05);and the venous blood and the half hour blood blood cell counts were compared,comparison of HGB,HCT,RWD-SD,RWD-CV,PLT PDW,P-LCR,MPV,LYMPH,BASO,etc,the difference was statistically significant(P < 0.05). There are differences between the index of the linear analysis,venous blood and theinstant blood, half an hour of arterial blood indicators are positively correlated with significant(P < 0.05). Conclusion Analysis of blood transfusion in emergency inspection of arterial blood gas, in the instant conditions analysis of alternative venous blood forblood cell count.%目的:探讨急诊动脉血气分析用血进行血细胞计数的可行性。方法选取2008年3月~2013年10月我院急诊科进行治疗的患者153例,所有患者同时进行动脉血气分析和静脉血细胞计数分析。结果静脉血与即时动脉血血细胞计数均数比较,MCHC、MCV、MCH、MPV、P-LCR、BASO、PDW等指标比较,差异有统计学意义(P<0.05);而静脉血与半小时动脉血血细胞计数均数比较,HGB、HCT、RWD-SD、RWD-CV、PLT、MPV、PDW、P-LCR、LYMPH、BASO等比较,差异有统计学意义(P<0.05)。对各项有差异的指标做线性分析,即时动脉血、半小时动脉血各项指标与静脉血呈正相关关系(P<0.05)。结论动脉血气分析用血急诊检验中,在即时条件下可以替代静脉血行全血细胞计数分析。

  12. Effect of VCO to leucocyte differential count, glucose levels and blood creatinine of hyperglycemic and ovalbumin sensitized Mus musculus Balb/c

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    RUBEN DHARMAWAN

    2009-03-01

    Full Text Available Abstract. Handajani NS, Dharmawan R. 2009. Effect of VCO to leucocyte differential count, glucose levels and blood creatinine of hyperglycemic and ovalbumin sensitized Mus musculus Balb/c. Nusantara Bioscience 1: 1-8. Chemical medicines and insulin can decrease glucose blood level on hyperglycemic patients with macro vascular side effect. Diabetes and allergy incidences are influenced by quality and quantity of leucocytes. Lauric acid within VCO reports decreased glucose blood level of diabetes and some allergy incidents. The purpose of the study is to know the effect of VCO on glucose blood level, differential leucocytes count and creatinine blood level on hyperglycemic and normoglicemic ovalbumin sensitized mice. Forty five (45 male (mice of Mus musculus Balb/c with average weight of 35 g are divided into 9 groups with 5 repetitions, those are 4 non alloxan groups and 5 alloxan induced hyperglycemic groups. On 22nd day to 36th day they are sensitize to ovalbumin as allergen. Blood sample was obtained by orbital vena using heparin as anti coagulant in order measuring glucose blood level by GOD method to 6 times, on 1st, 4th, 18th, 22nd, 32nd and 37th days, then are tested by ANOVA followed by DMRT 0.05. On 37th day, differential leucocytes are determined, blood level are counted, and then compared to normal value. The result of this study were that within differential leucocytes count of hyperglycemic mice, neutrophile percentage were much lower than the normal value (3.22%, and lymphocyte percentage were much higher than the normal value (94.54%. Consumed 0.003 mL/35 g VCO more 18 days decreased glucose blood level on hyperglycemic mice, decreased basophile percentage of ovalbumin sensitized mice, normalized neutrophile percentage no increased creatinine blood level.

  13. Correlation between the Improved Random Urine Red Blood Cell Count and the Addis Count of Urine Erythrocytes%改良的随机尿红细胞计数与Addis计数的相关性研究

    Institute of Scientific and Technical Information of China (English)

    姜俊; 章晓燕; 林静; 滕杰; 丁小强

    2013-01-01

    目的:研究随机尿红细胞计数经不同方法校正后与尿红细胞Addis计数的相关性.方法:以1t5例慢性肾脏病患者为研究对象,测定随机尿红细胞/尿比重、随机尿红细胞/渗透压和随机尿红细胞/尿肌酐,并分析它们与3h尿红细胞Addis计数的相关性.结果:随机尿红细胞计数、随机尿红细胞/比重、随机尿红细胞/渗透压和随机尿红细胞/尿肌酐与尿红细胞Ad-dis计数呈正相关(r=0.66、0.66、0.54、0.68,均P<0.01).当估算肾小球滤过率<60mL/(min· 1.73m2)、尿比重≤1.012时,随机尿红细胞/尿肌酐与尿红细胞Addis计数的相关性明显提高(r=0.77、0.76,均P<0.01).结论:随机尿红细胞/尿肌酐能较好地反映尿红细胞排泄量,尤其在低比重尿(尿比重≤1.012)和肾功能减退[(eGFR<60mL/(min·1.73m2))]时,它是评价尿红细胞排泄率的较好指标.%Objective:To study the correlation between improved random urinary red blood cell count after using different methods of correction and the Addis count of urinary erythrocytes.Methods:The ratios of the random urinary red blood count to the specific gravity,osmolality,and concentration of urinary creatinine in 115 patients with chronic kidney disease were detected,and the correlations between each of them and the Addis count of 3 hours urinary red cells were analyzed.Results:The random urinary red blood count,the ratios of the random urinary red blood count to the specific gravity,osmolality,and the concentration of urinary creatinine were positively correlated with the Addis count of urinary red blood cells,and the correlation coefficients were 0.66,0.66,0.54,0.68,respectively,P< 0.01.When the estimated glomerular filtration rate (eGFR) <60mL/(min · 1.73m2),the specific gravity of urine≤ 1.012,random urine red blood cell / creatinine and urine red blood cell count of Addis had significant positive correlation,and the correlation coefficients were 0

  14. Minimal sensor count approach to fuzzy logic rotary blood pump flow control.

    Science.gov (United States)

    Casas, Fernando; Ahmed, Nisar; Reeves, Andrew

    2007-01-01

    A rotary blood pump fuzzy logic flow controller without flow sensors was developed and tested in vitro. The controller, implemented in LabView, was set to maintain a flow set point in the presence of external pressure disturbances. Flow was estimated as a function of measured pump's delta P and speed, using a steady-state, nonlinear approximation. The fuzzy controller used the pump's flow estimate and delta P as feedback variables. The defuzzified control output manipulated the pump speed. Membership functions included flow error, delta P, and pump speed. Experimental runs in a mock loop (water/glycerin 3.5 cPs, 37 degrees C), using the estimated flow, were compared with those using a Transonic flow meter for nine conditions of flow and delta P (4 to 6 L/min, 150 to 350 mm Hg). Pressure disturbances generated by a servo pinch valve ranged from +/-23 to +/-47 mm Hg. Results indicated that the fuzzy controller ably regulated the flow set point to within +/-10% of the baseline even under large swings in pressure. There was no difference in controller performance between the ultrasonic flow measurement and the estimated flow calculation scenarios. These tests demonstrated that the fuzzy controller is capable of rejecting disturbances and regulating flow to acceptable limits while using a flow estimate.

  15. A study of eosinophil count in nasal and blood smear in allergic respiratory diseases in a rural setup

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    Naveen Kumar

    2012-01-01

    Full Text Available Allergic respiratory disorders are fairly common visiting cases in pediatrics outpatient department (OPD. With an appropriate history and detailed examination, diagnosis may not be problematic. Routine investigation may not contribute much to the final diagnosis but may help in ruling out other possibilities. This study was done to evaluate sensitivity and specificity of blood or nasal eosinophilia in subjects suffering from allergic respiratory disorders and also to assess the feasibility of nasal cytogram which is a simple, economical and reliable investigation in allergic respiratory disorders. This is a prospective clinical correlation study of patients attending outpatient department. 100 subjects aged between 2-18 years of either sex were selected for the estimation of eosinophil count in nasal and peripheral smear in allergic respiratory disorders. All allergic respiratory cases based on eosinophillia. The nasal and blood eosinophilia were compared with each other and clinical findings of allergic rhinitis with or without asthma were studied. In this study peak age incidence was seen between 11-18 years and it was more common in males. Rhinorrhoea, pale mucosa and nasal obstruction were common findings in allergic rhinitis with bronchial asthma. Nasal eosinophilia was seen in 52.4% and 64.9% of cases of allergic rhinitis and allergic rhinitis with asthma respectively. Blood eosinophilia was seen in 54% and 56.8% of cases of allergic rhinitis with asthma respectively. Nasal cytogram which is a simple, economical and non- invasive procedure can be used as an alternative to invasive peripheral smear eosinophilia as both are equally efficacious in diagnosing allergic respiratory diseases.

  16. Subnormal Peripheral Blood Leukocyte Counts Are Related to the Lowest Prevalence and Incidence of Metabolic Syndrome: Tianjin Chronic Low-Grade Systemic Inflammation and Health Cohort Study

    Science.gov (United States)

    Sun, Shaomei; Wu, Hongmei; Zhang, Qing; Wang, Chongjin; Guo, Yinting; Du, Huanmin; Liu, Li; Jia, Qiyu; Wang, Xing; Song, Kun

    2014-01-01

    Few studies have assessed the relationship between a subnormal inflammatory status and metabolic syndrome (MS). We therefore designed a cross-sectional and 5-year cohort study to evaluate how a subnormal peripheral blood leukocyte count is related to MS. Participants were recruited from Tianjin Medical University General Hospital-Health Management Centre. Both a baseline cross-sectional (n = 46,179) and a prospective assessment (n = 13,061) were performed. Participants without a history of MS were followed up for 5 years. Leukocyte counts and MS components were assessed at baseline and yearly during the follow-up. Adjusted logistic and Cox proportional hazards regression models were used to assess relationships between the categories of leukocyte counts and MS. The subnormal leukocyte counts group (1,100–3,900 cells/mm3) had the lowest prevalence and incidence of MS. The odds ratio and hazard ratio (95% confidence interval) of the highest leukocyte counts were 1.98 (1.57–2.49) and 1.50 (1.22–1.84) (both P for trend <0.0001), respectively, when compared to the subnormal leukocyte counts group after adjusting for potential confounders. This study has shown that subnormal leukocyte counts are independently related to the lowest prevalence and incidence of MS. The findings suggest that it is necessary to restudy and discuss the clinical or preventive value of subnormal leukocyte counts. PMID:24876672

  17. Subnormal Peripheral Blood Leukocyte Counts Are Related to the Lowest Prevalence and Incidence of Metabolic Syndrome: Tianjin Chronic Low-Grade Systemic Inflammation and Health Cohort Study

    Directory of Open Access Journals (Sweden)

    Shaomei Sun

    2014-01-01

    Full Text Available Few studies have assessed the relationship between a subnormal inflammatory status and metabolic syndrome (MS. We therefore designed a cross-sectional and 5-year cohort study to evaluate how a subnormal peripheral blood leukocyte count is related to MS. Participants were recruited from Tianjin Medical University General Hospital-Health Management Centre. Both a baseline cross-sectional (n=46,179 and a prospective assessment (n=13,061 were performed. Participants without a history of MS were followed up for 5 years. Leukocyte counts and MS components were assessed at baseline and yearly during the follow-up. Adjusted logistic and Cox proportional hazards regression models were used to assess relationships between the categories of leukocyte counts and MS. The subnormal leukocyte counts group (1,100–3,900 cells/mm3 had the lowest prevalence and incidence of MS. The odds ratio and hazard ratio (95% confidence interval of the highest leukocyte counts were 1.98 (1.57–2.49 and 1.50 (1.22–1.84 (both P for trend <0.0001, respectively, when compared to the subnormal leukocyte counts group after adjusting for potential confounders. This study has shown that subnormal leukocyte counts are independently related to the lowest prevalence and incidence of MS. The findings suggest that it is necessary to restudy and discuss the clinical or preventive value of subnormal leukocyte counts.

  18. Elevated White Blood Cell Count Is Associated with Higher Risk of Glucose Metabolism Disorders in Middle-Aged and Elderly Chinese People

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    Hua Jiang

    2014-05-01

    Full Text Available White blood cell (WBC count has been associated with diabetic risk, but whether the correlation is independent of other risk factors has hardly been studied. Moreover, very few such studies with large sample sizes have been conducted in Chinese. Therefore, we investigated the relationship between WBC count and glucose metabolism in china. We also examined the relevant variables of WBC count. A total of 9,697 subjects (mean age, 58.0 ± 9.1 years were recruited. The subjects were classified into four groups, including subjects with normal glucose tolerance, isolated impaired fasting glucose, impaired glucose tolerance and type 2 diabetes mellitus (T2DM. We found that WBC count increased as glucose metabolism disorders exacerbated. WBC count was also positively correlated with waist hip ratio, body mass index, smoking, triglycerides, glycosylated haemoglobin A1c (HbA1c and 2-h postprandial glucose. In addition, high density lipoprotein and the female gender were inversely correlated with WBC count. In patients with previously diagnosed T2DM, the course of T2DM was not correlated with WBC count. Our findings indicate that elevated WBC count is independently associated with worsening of glucose metabolism in middle-aged and elderly Chinese. In addition, loss of weight, smoking cessation, lipid-modifying therapies, and control of postprandial plasma glucose and HbA1c may ameliorate the chronic low-grade inflammation.

  19. Era of blood component therapy: time for mandatory pre-donation platelet count for maximizing donor safety and optimizing quality of platelets.

    Science.gov (United States)

    Das, Sudipta Sekhar; Zaman, R U; Biswas, Dipak

    2013-12-01

    Blood bank regulatory agencies including the Drug and Cosmetics Act (DCA) of India do not mandate a predonation platelet count in whole blood donation. Mandating such practice will definitely optimize the quality of random donor platelets (RDP) in terms of platelet yield and patient therapeutic benefit. We observed poor platelet yield in RDP concentrates prepared at our center with a significant number not meeting the DCA guideline of ≥ 4.5 × 10(10) per bag processed from 450 ml of whole blood. Therefore we planned this study to evaluate the pre-donation hematological values in our blood donor population and effect of these values on the quality of platelet concentrates. The prospective study included 221 blood donors eligible for donating 450 ml of whole blood (WB). Following the departmental standard operating procedure (SOP) RDPs were prepared using the 'Top & Bottom' quadruple bag system and automated component extractor. Quality of RDP was assessed as per departmental protocol. All results were recorded and subsequently transcribed to SPSS working sheet. A significant (pblood counts has been observed after WB donation. Mean donor Hb and platelets reduced by 0.72 g/dl and 22.1 × 10(6)/ml respectively. Quality of RDPs in terms of platelet yield was significantly better (pcount was >200 × 10(6)/ml. Although platelet yield significantly correlated with the donor platelet count however quality of RDPs in terms of red cell contamination showed no correlation with the donor hematocrit. Platelet yield in random donor platelets is a concern in Eastern India. A platelet yield of 4.5 × 10(10) per bag as mandated by the DCA of India was only achieved when the donor platelet count was >200 × 10(6)/ml. Posttransfusion platelet recovery (PPR) was unsatisfactory in the transfused patient. Introduction of pre-donation platelet count in whole blood donation will maximize donor safety and optimize patient platelet transfusion management.

  20. STUDY OF CHANGES IN HAEMOGLOBIN LEVEL AND LEUKOCYTE COUNTS IN CORD BLOOD OF NEWBORNS OF HYPERTENSIVE MOTHERS IN AGMC AND GB PANT HOSPITAL

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    Evalyn

    2014-11-01

    Full Text Available AIMS AND OBJECTIVES: To observe the change in haemoglobin level and leukocyte counts in the cord blood of neonates born of hypertensive mothers by comparing it with the haemoglobin level and leukocyte counts of cord blood of neonates born of healthy mothers. MATERIALS AND METHODS: A Prospective observational study was carried out among thirty newborns of hypertensive mothers and thirty apparently healthy newborns of normotensive mothers in, West Tripura. Haemoglobin level was estimated by Sahli’s method and total Leukocyte count (TLC was done using Improved Neubauer’s Counting chamber. Absolute counts were obtained by doing Differential Leukocyte Count (DLC and the value obtained from TLC. For DLC, slides were stained with Leishman’s stain. Statistical analysis was carried out using t-test for the comparison of differences of the means by SPSS 15.0. RESULTS: Haemoglobin level was found to be significantly higher in the study group as compared to control group. Leukopenia, neutropenia, lymphopenia and anaemia were found in 10%, 3.3%, 13.3% and 23.3% subjects respectively in the study group. Leukopenia, lymphopenia and anaemia were also found in 6.6%, 10% and 56.6% subjects respectively in the control group. CONCLUSION: A significant haematological changes occur in the newborns of hypertensive mothers and further research need to be carried out to find the relation between hypertensive disorders and changes in haematological parameters of the newborns.

  1. Influence of the first radioactive iodine ablation on peripheral complete blood count in patients with differentiated thyroid cancer.

    Science.gov (United States)

    Hu, Tianpeng; Meng, Zhaowei; Zhang, Guizhi; Jia, Qiang; Tan, Jian; Zheng, Wei; Wang, Renfei; Li, Xue; Liu, Na; Zhou, Pingping; Upadhyaya, Arun

    2016-08-01

    Radioactive iodine (RAI) is considered to be related with hematologic changes. This study aimed to evaluate influence of the first RAI ablation on peripheral complete blood count (CBC) in patients with differentiated thyroid cancer (DTC).Data of CBC at baseline and 6 months after RAI were obtained in 385 patients with DTC with approximately 3700 MBq I (ranging 2220-7585 MBq). Further comparison was done in 196 patients with 1-month postablation data available. Routine blood examinations were performed to determine impact of RAI on white blood cell (WBC), red blood cell (RBC), hemoglobin, platelet, neutrophil, lymphocyte, and monocyte in both sexes. Continuous variables were compared by paired t tests and independent samples t test, and categorical variables were compared by chi-square analysis. Data with repeated measurements were analyzed by analysis of variance.The first RAI after thyroidectomy was associated with mild, yet significant declines in WBC, platelet, and lymphocyte, which persisted for 6 months. One month after RAI, significant declines were found in all CBC, including RBC and hemoglobin (all P < 0.05). While CBC partly recovered 6 months after RAI, this follow-up CBC still demonstrated significant declines in WBC, platelet, and lymphocyte (all P < 0.05) without gender differences. Significant rises in RBC and hemoglobin in males and females were found. The decline of platelet in females was more obvious than in males at 3700 to 4440 MBq of RAI. On the contrary, the rises of RBC and hemoglobin in males were higher than in females. There were no significant complications during the follow-up.WBC and platelet decreased obviously 1 month after RAI. While they partly recovered 6 months after RAI, they were still lower than the baseline. However, RBC and hemoglobin transiently decreased at 1 month and then increased to levels even higher than baseline 6 months later. At 3700 to 4440 MBq of RAI, the decline of platelet in females was more obvious

  2. Eosinophilia in routine blood samples as a biomarker for solid tumor development - A study based on the Copenhagen Primary Care Differential Count (CopDiff) Database

    DEFF Research Database (Denmark)

    Andersen, Christen Lykkegaard; Siersma, Volkert Dirk; Hasselbalch, Hans Carl;

    2014-01-01

    eosinophilia in routine blood samples as a potential biomarker of solid tumor development in a prospective design. MATERIAL AND METHODS: From the Copenhagen Primary Care Differential Count (CopDiff) Database, we identified 356 196 individuals with at least one differential cell count (DIFF) encompassing...... the eosinophil count during 2000-2007. From these, one DIFF was randomly chosen and categorized according to no (... was increased with mild eosinophilia [OR 1.93 (CI 1.29-2.89), p = 0.0013]. No associations with eosinophilia were observed for the remaining solid cancers. CONCLUSION: We demonstrate that eosinophilia in routine blood samples associates with an increased risk of bladder cancer. Our data emphasize...

  3. Comparison of usefulness of C-reactive protein versus white blood cell count to predict outcome after primary percutaneous coronary intervention for ST elevation myocardial infarction

    NARCIS (Netherlands)

    Smit, Jaap Jan J.; Ottervanger, Jan Paul; Slingerland, Robbert J.; Kolkman, J. J. Evelien; Suryapranata, Harry; Hoorntje, Jan C. A.; Dambrink, Jan-Henk E.; Gosselink, A. T. Marcel; de Boer, Menko-Jan; Zijlstra, Felix; van 't Hof, Arnoud W. J.

    2008-01-01

    White blood cell (WBC) count and high-sensitive C-reactive protein (hs-CRP) are both used as markers of inflammation and prognosis after an ST elevation myocardial infarction (STEMI), but it is unknown whether they have independent prognostic value. We investigated the association and independent pr

  4. Effect of surgical castration of bull calves at different stages of maturity with or without analgesia on the acute phase response (APR) and complete blood count (CBC)

    Science.gov (United States)

    The study objective was to determine if surgical castration at birth or weaning impacts the acute phase response (APR) or complete blood counts (CBC) and whether concurrent administration of an oral analgesic (meloxicam) ameliorates inflammation. Bull calves (n=29) from the University of Arkansas re...

  5. Reference intervals of complete blood count constituents are highly correlated to waist circumference: Should obese patients have their own "normal values?"

    NARCIS (Netherlands)

    Vuong, J.; Qiu, Y.; La, M.; Clarke, G.; Swinkels, D.W.; Cembrowski, G.

    2014-01-01

    Body mass index (BMI), the prevalent indicator of obesity, is not easily grasped by patients nor physicians. Waist circumference (WC) is correlated to obesity, is better understood and has a stronger relationship to the metabolic syndrome. We compiled WC, complete blood count (CBC) parameters as wel

  6. Complete Blood Count

    Science.gov (United States)

    ... more concentrated inside the red cells, such as autoimmune hemolytic anemia, in burn patients, and hereditary spherocytosis, a rare ... Platelet autoantibody Drugs (acetaminophen, quinidine, sulfa drugs) Cirrhosis Autoimmune ... disorder (e.g., essential thrombocythemia) MPV ( ...

  7. Interleukin-2 dose, blood monocyte and CD25+ lymphocyte counts as predictors of clinical response to interleukin-2 therapy in patients with renal cell carcinoma

    DEFF Research Database (Denmark)

    Hermann, G G; Geertsen, P F; von der Maase, H

    1991-01-01

    The purpose of this study was to determine immunological parameters in the peripheral blood that correlate with the clinical effect of interleukin-2 (IL-2) in patients with metastatic renal cell cancer. A group of 26 patients with metastatic renal cell cancer underwent IL-2 treatment using a 36-day...... schedule with continuous intravenous IL-2 infusion (3 x 10(6) units m-2 day-1) administered from days 1 to 5 and days 12 to 16. The white blood cell count and the absolute and relative number of neutrophils, lymphocytes, eosinophils and monocytes were recorded six times in peripheral blood during...

  8. CELL RESPIRATION STUDIES : II. A COMPARATIVE STUDY OF THE OXYGEN CONSUMPTION OF BLOOD FROM NORMAL INDIVIDUALS AND PATIENTS WITH INCREASED LEUCOCYTE COUNTS (SEPSIS; CHRONIC MYELOGENOUS LEUCEMIA).

    Science.gov (United States)

    Daland, G A; Isaacs, R

    1927-06-30

    1. The oxygen consumption of blood of normal individuals, when the hemoglobin is saturated with oxygen, is practically zero within the limits of experimental error of the microspirometer used. 2. The oxygen consumed in a microspirometer by the blood of patients with chronic myelogenous leucemia with a high white blood cell count, and of one with leucocytosis from sepsis, was proportional to the number of adult polymorphonuclear neutrophils in the blood. 3. No correlation could be made between the rate of oxygen absorption and the total number of white blood cells in the blood, or the total number of immature cells, or the number of red blood cells, or the amount of oxyhemoglobin. 4. The blood of patients with chronic myelogenous leucemia continued to use oxygen in the microspirometer longer than that of normal individuals, and the hemoglobin, in the leucemic bloods, became desaturated even though exposed to air. 5. In blood in which the bulk. of the cells were immature and the mature cells few, the oxygen consumption was lower than in blood in which the mature cells predominated. The rate of oxygen consumption of the immature cells was relatively low as compared to the mature. 6. The slower rate of oxygen absorption by the immature leucocytes in chronic myelogenous leucemia as compared to the mature cells, places them, in accord with Warburg's reports, in the class of the malignant tissues in this respect rather than in the group of young or embryonic cells.

  9. POSSIBLE ADVERSE EFFECTS OF ONCE-DAILY ORAL THERAPEUTIC DOSE OF EITHER GLUCOSAMINE SULFATE OR GLUCOSAMINE/CHONDROITIN SULFATE ON BLOOD CELLS COUNT IN RATS

    Directory of Open Access Journals (Sweden)

    Noushi Abeer Amer

    2013-10-01

    Full Text Available This study was designed to investigate the possible adverse effects that may be induced by once-daily therapeutic doses of either glucosamine sulfate or glucosamine/chondroitin sulfate administered orally to rats for 30 days on blood cells (RBCs, WBCs and platelets counts. Forty three white healthy adult Albino rats of both sexes were selected randomly for this study. They were divided into three groups (І, ІІ, ІІІ. Group І received 0.05 ml distilled water, group ІІ received once daily therapeutic dose of glucosamine sulphate and group ІІІ received once daily therapeutic dose of glucosamine sulphate/chondroitin sulphate orally. The treatment period was for 30 days. At day 31, the animals were subjected to light ether anaesthesia and blood was withdrawn from the eye by retro-orbital puncture for the estimation of blood cells (RBCs, WBCs and platelets count. Treatment with single daily therapeutic dose of either GS alone or GS/CS for 30 days on blood cells count in rats produced a non significant change in RBCs counts compared to control and to each other. There were no statistically significant differences in total WBCs count at day 31 in animals administered once daily therapeutic dose of either GS or GS/CS orally compared to control group. In contrast, there was a statistically significant elevation in total WBCs count in GS/CS- treated rats compared to that in the GS-treated rats. The results of this study also showed that there was statistically significant decrease in neutrophils percentage in both drug treatment groups compared to control group. A statistically significant reduction in the percentage of monocytes was observed in GS/CS group compared to the corresponding percentage in animals of control group; while, there were non-significant differences in the percentage of monocytes in GS treated rats compared to that in the control group. There were no significant differences in the percentage of monocytes at day 31 of GS

  10. Cell type specific DNA methylation in cord blood: A 450K-reference data set and cell count-based validation of estimated cell type composition.

    Science.gov (United States)

    Gervin, Kristina; Page, Christian Magnus; Aass, Hans Christian D; Jansen, Michelle A; Fjeldstad, Heidi Elisabeth; Andreassen, Bettina Kulle; Duijts, Liesbeth; van Meurs, Joyce B; van Zelm, Menno C; Jaddoe, Vincent W; Nordeng, Hedvig; Knudsen, Gunn Peggy; Magnus, Per; Nystad, Wenche; Staff, Anne Cathrine; Felix, Janine F; Lyle, Robert

    2016-09-01

    Epigenome-wide association studies of prenatal exposure to different environmental factors are becoming increasingly common. These studies are usually performed in umbilical cord blood. Since blood comprises multiple cell types with specific DNA methylation patterns, confounding caused by cellular heterogeneity is a major concern. This can be adjusted for using reference data consisting of DNA methylation signatures in cell types isolated from blood. However, the most commonly used reference data set is based on blood samples from adult males and is not representative of the cell type composition in neonatal cord blood. The aim of this study was to generate a reference data set from cord blood to enable correct adjustment of the cell type composition in samples collected at birth. The purity of the isolated cell types was very high for all samples (>97.1%), and clustering analyses showed distinct grouping of the cell types according to hematopoietic lineage. We explored whether this cord blood and the adult peripheral blood reference data sets impact the estimation of cell type composition in cord blood samples from an independent birth cohort (MoBa, n = 1092). This revealed significant differences for all cell types. Importantly, comparison of the cell type estimates against matched cell counts both in the cord blood reference samples (n = 11) and in another independent birth cohort (Generation R, n = 195), demonstrated moderate to high correlation of the data. This is the first cord blood reference data set with a comprehensive examination of the downstream application of the data through validation of estimated cell types against matched cell counts.

  11. The Alterations of Serum Cortisol Level and Blood Cell Count in Male Rats after a Short Term Exposure to Burned Radioactive Lantern Mantle Powder

    Directory of Open Access Journals (Sweden)

    Rezaeian Mohsen

    2010-03-01

    Full Text Available Background: Most lantern mantles contain low levels of radioactive thorium. Although radioactive lantern mantles present a minimal radiation health hazard, it is generally believed when inhaled or ingested, it can be dangerous. The purpose of this study was to assess the effect of short term exposure to radioactive lantern mantle on serum cortisol level and blood count.Materials and Methods: This experimental study was conducted in 2007-2009 in the school of medicine of Rafsanjan University of Medical Sciences. Twenty eight rats were divided randomly into two groups of 14 animals each. The first group was exposed to 600 mg burned radioactive lantern mantle powder (activity of 800Bq for 24 hours and inhaled radon released from mantles. The second group was exposed to non-radioactive lantern mantle powder at the same interval. Paired t-test was used to evaluate the difference in the means of cortisol and blood cell count in both groups. P<0.05 was considered as the significance level.Results: Short term exposure of animals to radioactive lantern mantle powder led to a statistically significant decreased cortisol level, while no statistically significant decrease was found in animals that were exposed to non-radioactive mantle powder. Furthermore, a significant reduction was shown in post-exposure counts of WBC in the case group.Conclusions: Despite alteration of serum cortisol level, this study could not show stimulatory effects in some blood counts

  12. Total and differential white blood cell counts, high-sensitivity C-reactive protein, and the metabolic syndrome in non-affective psychoses.

    Science.gov (United States)

    Miller, Brian J; Mellor, Andrew; Buckley, Peter

    2013-07-01

    The metabolic syndrome is highly prevalent in patients with schizophrenia, and is associated with a state of chronic, low-grade inflammation. Schizophrenia is also associated with increased inflammation, including aberrant blood levels of pro-inflammatory cytokines and high-sensitivity C-reactive protein (hsCRP). The purpose of this study is to investigate the relationship between total and differential white blood cell (WBC) counts, hsCRP, and the metabolic syndrome in patients with schizophrenia and related non-affective psychoses. Fifty-nine inpatients and outpatients age 18-70 with non-affective psychotic disorders and 22 controls participated in this cross-sectional study. Subjects had a fasting blood draw between 8 and 9 am for glucose, lipids, total and differential WBC counts, and hsCRP. Vital signs and anthropometric measures were obtained. Patients with non-affective psychosis and the metabolic syndrome had significantly higher total WBC counts, monocytes, and hsCRP levels than patients without the metabolic syndrome (p≤0.04 for each). In binary logistic regression analyses, after controlling for potential confounding effects of age, race, sex, age at first hospitalization for psychosis, parental history of diabetes, smoking, and psychotropic medications, total WBC count, monocytes, and hsCRP were significant predictors of metabolic syndrome in patients (p≤0.04 for each). hsCRP was also a significant predictor of increased waist circumference and triglycerides in patients (p≤0.05 for each). Our findings suggest that measurement of total and differential WBC counts and hsCRP blood levels may be germane to the clinical care of patients with schizophrenia and related disorders, and support an association between inflammation and metabolic disturbance in these patients.

  13. Association between circulating white blood cell count and long-term incidence of age-related macular degeneration: the Blue Mountains Eye Study.

    Science.gov (United States)

    Shankar, Anoop; Mitchell, Paul; Rochtchina, Elena; Tan, Jennifer; Wang, Jie Jin

    2007-02-15

    Inflammatory processes are implicated in the development and progression of age-related macular degeneration (AMD). However, there are limited data on longitudinal associations between systemic markers of inflammation and AMD. The authors examined the prospective relation between the circulating white blood cell (WBC) count and early and late AMD in a population-based cohort of 3,654 participants, aged 49-97 years, in the Blue Mountains region, Australia. The main outcome of interest was the 10-year incidence of early and late AMD among individuals free from corresponding disease at the baseline (1992-1994). An elevated baseline WBC count was associated with early AMD incidence, independent of smoking and other major confounders. The multivariable relative risk comparing tertile 3 of WBC count (>6.7 x 10(9) cells/liter) with tertile 1 (count and early AMD was present consistently in analyses of different early AMD lesions, including incident pigmentary abnormalities and soft indistinct/reticular drusen. Moreover, this association persisted in subgroup analyses by gender and smoking. An elevated WBC count at baseline was not consistently associated with late AMD incidence. This study provides population-based evidence supporting a longitudinal association between the circulating WBC count, a widely available marker of inflammation, and incidence of early AMD.

  14. The prognostic impact of preoperative blood monocyte count in pathological T3N0M0 rectal cancer without neoadjuvant chemoradiotherapy.

    Science.gov (United States)

    Zhang, Lu-Ning; Xiao, Weiwei; OuYang, Pu-Yun; You, Kaiyun; Zeng, Zhi-Fan; Ding, Pei-Rong; Pan, Zhi-Zhong; Xu, Rui-Hua; Gao, Yuan-Hong

    2015-09-01

    It remains controversial whether adjuvant therapy should be delivered to pathological T3N0M0 rectal cancer without neoadjuvant chemoradiotherapy. Thus identification of patients at high risk is of particular importance. Herein, we aimed to evaluate whether the absolute peripheral blood monocyte count can stratify the pathological T3N0M0M0 rectal cancer patients in survival. A total of 270 pathological T3N0M0 rectal cancer patients with total mesorectal excision-principle radical resection were included. The optimal cut-off value of preoperative monocyte count was determined by receiver operating characteristic curve analysis. Overall survival and disease-free survival between low- and high-monocyte were estimated by Kaplan-Meier method and Cox regression model. The optimal cut-off value for monocyte count was 595 mm(3). In univariate analysis, patients with monocyte counts higher than 595/mm(3) had significantly inferior 5-year overall survival (79.2 vs 94.2 %, P = 0.006) and disease-free survival (67.8 vs 86.0 %, P count remained to be associated with poor overall survival (HR = 2.55, 95 % CI 1.27-5.10; P = 0.008) and disease-free survival (HR = 2.63, 95 % CI 1.48-4.69; P = 0.001). Additionally, the significant association of monocyte count with disease-free survival was hardly influenced in the subgroup analysis, whereas this correlation was restricted to the males and patients with normal carcinoembryonic antigen (CEA) level (count is independently predictive of worse survival of pathological T3N0M0 rectal cancer patients without neoadjuvant chemoradiotherapy. Postoperative adjuvant therapy might be considered for patients with high-monocyte count.

  15. Are the review criteria for automated complete blood counts of the International Society of Laboratory Hematology suitable for all hematology laboratories?

    OpenAIRE

    Samuel Ricardo Comar; Mariester Malvezzi; Ricardo Pasquini

    2014-01-01

    OBJECTIVE: to verify whether the review criteria for automated blood counts suggested by the International Consensus Group for Hematology Review of the International Society for Laboratory Hematology are suitable for the Hematology Laboratory of Hospital de Clinicas, Universidade Federal do Paraná.METHODS: initially, the review criteria of the International Society for Laboratory Hematology were adapted due to limitations in the Institution's electronic hospital records and interfacing system...

  16. Effect of Venous Blood and Peripheral Blood on Platelet Count%静脉采血和末梢采血对血小板计数的影响

    Institute of Scientific and Technical Information of China (English)

    薛可

    2015-01-01

    Objective Impact analysis of peripheral venous blood and blood platelet count. Methods 30 patients taking the hospital from October 2012 to October 2014 to detect blood of all patients receiving peripheral blood and blood collection. At the same time the use of automated blood cell analyzer for two kinds of venous blood and peripheral blood collection methods for scientiifc testing. Results The number of blood platelets would cause different types of methods of direct influence, t=6.57, significant statistical significance (P<0.05). Conclusion Thrombocytopenia, blood velocity, counting the number of cells and other factors are the main factors that affect platelet count results. Venous blood and peripheral blood platelet count also has a certain impact.%目的:研究分析静脉采血和末梢采血对血小板计数的影响。方法选取本院2012年10月~2014年10月检测血常规的30例患者,全部患者接受末梢血以及静脉血的采集。与此同时使用血细胞自动分析仪对静脉采血和末梢采血两种采集方法进行科学的检测。结果不同类型的采血方法会对血小板数目造成直接性的影响,t=6.57,具有显著性统计学意义(P<0.05)。结论血小板减少症、采血速度、计数格数等因素是影响小板计数结果的主要因素。静脉采血和末梢采血对血小板计数也具有一定的影响。

  17. Effect of supplements: Probiotics and probiotic plus honey on blood cell counts and serum IgA in patients receiving pelvic radiotherapy

    Directory of Open Access Journals (Sweden)

    Hajar-Alsadat Mansouri-Tehrani

    2015-01-01

    Full Text Available Background: Radiotherapy is frequently used in treatment approaches of pelvic malignancies. Nevertheless, it has some known systemic effects on blood cells and the immune system that possibly results in their susceptibility to infection. Probiotics are live microbial food ingredients that provide a health advantage to the consumer. Honey has prebiotic properties. The aim of this clinical trial was to investigate probable effects of probiotic or probiotics plus honey on blood cell counts and serum IgA levels in patients receiving pelvic radiotherapy. Materials and Methods: Sixty-seven adult patients with pelvic cancer were enrolled. Patients were randomized to receive either: (1 Probiotic capsules (including: Lactobacillus casei, Lactobacillus acidophilus, Lactobacillus rhamnosus, Lactobacillus bulgaricus, Bifidobacterium breve, Bifidobacterium longum, and Streptococcus thermophiles (n = 22, (2 probiotic capsules plus honey (n = 21 or (3 placebo capsules (n = 24 all for 6 weeks. Blood and serum samples were collected for one week before radiotherapy and 24-72 h after the end of radiotherapy. Results: White blood cells (WBC, red blood cells (RBC, platelet counts, and serum IgA level were not significantly changed in patients taking probiotic (alone or plus honey during pelvic radiotherapy. The mean decrease in RBC count was 0.52, 0.18, and 0.23 × 10 6 cells/μL, WBC count was 2.3, 1.21, and 1.34 × 10 3 cells/μL and platelet count was, 57.6, 53.3, and 66.35 × 10 3 cells/μL for the probiotic, probiotic plus honey, and placebo groups, respectively. The mean decrease of serum IgA was 22.53, 29.94, and 40.73 mg/dL for the probiotic, probiotic plus honey, and placebo groups, respectively. Conclusion: The observed nonsignificant effect of probiotics may be in favor of local effects of this product in the gut rather than systemic effects, however, as a trend toward a benefit was indicated, further studies are necessary in order to extract effects of

  18. 红细胞计数与肝功能状态的关联性分析%Correlation between red blood cell count and liver function status

    Institute of Scientific and Technical Information of China (English)

    谢宵梦; 王雷婕; 姚明解; 文夏杰; 陈香梅; 尤红; 贾继东; 赵景民; 鲁凤民

    2016-01-01

    目的 比较不同肝病患者的红细胞计数的变化,探讨红细胞计数与肝损害程度的关系.方法 收集1 427例原发性肝癌患者,172例肝硬化患者和185例肝炎患者的临床资料,并对其进行Child-Pugh分级.回顾性分析不同肝病患者红细胞计数的差异,探讨红细胞计数与肝功能状态的关联.据资料不同分别采用Mann-Whitney U检验、Kruskal Wallis H检验、秩和检验、Spearman秩和相关检验、x2检验.结果 慢性肝炎患者、肝癌患者和肝硬化患者的红细胞计数差异具有统计学意义,且慢性肝炎>肝癌>肝硬化(P< 0.05).肝硬化患者中,红细胞计数在Child-Pugh分级较高的患者中倾向于降低(P<0.05). 结论 红细胞计数可反映肝硬化患者的肝损害程度,或可为完善肝硬化患者的肝功能预测模型提供依据.%Objective To investigate the changes in red blood cell count in patients with different liver diseases and the correlation between red blood cell count and degree of liver damage.Methods The clinical data of 1427 patients with primary liver cancer,172 patients with liver cirrhosis,and 185 patients with hepatitis were collected,and the Child-Pugh class was determined for all patients.The differences in red blood cell count between patients with different liver diseases were retrospectively analyzed,and the correlation between red blood cell count and liver function status was investigated.The Mann-Whitney U test,KruskalWallis H test,rank sum test,Spearman rank sum correlation test,and chi-square test were performed for different types of data.Results Red blood cell count showed significant differences between patients with chronic hepatitis,liver cancer,and liver cirrhosis and was highest in patients with chronic hepatitis and lowest in patients with liver cirrhosis (P < 0.05).In the patients with liver cirrhosis,red blood cell count tended to decrease in patients with a higher Child-Pugh class (P < 0.05).Conclusion For

  19. Transcriptional regulation of human thromboxane synthase gene expression

    Energy Technology Data Exchange (ETDEWEB)

    Lee, K.D.; Baek, S.J.; Fleischer, T [Univ. of Maryland Medical School, Baltimore, MD (United States)] [and others

    1994-09-01

    The human thromboxane synthase (TS) gene encodes a microsomal enzyme catalyzing the conversion of prostaglandin endoperoxide into thromboxane A{sub 2}(TxA{sub 2}), a potent inducer of vasoconstriction and platelet aggregation. A deficiency in platelet TS activity results in bleeding disorders, but the underlying molecular mechanism remains to be elucidated. Increased TxA{sub 2} has been associated with many pathophysiological conditions such as cardiovascular disease, pulmonary hypertension, pre-eclampsia, and thrombosis in sickle cell patients. Since the formation of TxA{sub 2} is dependent upon TS, the regulation of TS gene expression may presumably play a crucial role in vivo. Abrogation of the regulatory mechanism in TS gene expression might contribute, in part, to the above clinical manifestations. To gain insight into TS gene regulation, a 1.7 kb promoter of the human TS gene was cloned and sequenced. RNase protection assay and 5{prime} RACE protocols were used to map the transcription initiation site to nucleotide A, 30 bp downstream from a canonical TATA box. Several transcription factor binding sites, including AP-1, PU.1, and PEA3, were identified within this sequence. Transient expression studies in HL-60 cells transfected with constructs containing various lengths (0.2 to 5.5 kb) of the TS promoter/luciferase fusion gene indicated the presence of multiple repressor elements within the 5.5 kb TS promoter. However, a lineage-specific up-regulation of TS gene expression was observed in HL-60 cells induced by TPA to differentiate along the macrophage lineage. The increase in TS transcription was not detectable until 36 hr after addition of the inducer. These results suggest that expression of the human TS gene may be regulated by a mechanism involving repression and derepression of the TS promoter.

  20. Changes of nitric oxide and endothelin, thromboxane A2 and prostaglandin in cirrhotic patients undergoing liver transplantation

    Institute of Scientific and Technical Information of China (English)

    Zi-Qing Hei; He-Qing Huang; Chen-Fang Luo; Shang-Rong Li; Gang-Jian Luo

    2006-01-01

    AIM: To investigate the perioperative changes of nitric oxide (NO) and endothelin (ET), thromboxane A2 (TXA2) and prostaglandin (PGI2) during liver transplantation in end-stage liver disease patients.METHODS: Twenty-seven patients with end-stage cirrhosis undergoing liver transplantation were enrolled in this prospective study. Blood samples were obtained from superior vena at five different surgical stages.Plasma concentrations of nitrate and nitrite were determined to reflect plasma NO levels. Plasma levels of ET-1,6-keto-PGF1 alpha and thromboxane B2 (TXB2),the latter two being stable metabolites of PGI2 and TXA2 respectively, were measured.RESULTS: The NO level decreased significantly after vascular cross-clamping and increased significantly at 30 min after reperfusion. While the ET levels at 30 min after clamping and after reperfusion were significantly elevated. The ratio of NO/ET decreased significantly at 30 min after vascular cross-clamping and at the end of surgery. The PGI2 level and the TXA2 during liver transplantation were significantly higher than the baseline level, but the ratio of TXA2/PGI2 decreased significantly at 30 min after clamping.CONCLUSION: NO/ET and TXA2/PGI2 change during liver transplantation. Although the precise mechanism remains unknown, they may play a role in the pathobiology of a variety of liver transplant-relevant processes.

  1. Erythrocytes and the regulation of human skeletal muscle blood flow and oxygen delivery: role of erythrocyte count and oxygenation state of haemoglobin.

    Science.gov (United States)

    González-Alonso, José; Mortensen, Stefan P; Dawson, Ellen A; Secher, Niels H; Damsgaard, Rasmus

    2006-04-01

    Blood flow to dynamically contracting myocytes is regulated to match O(2) delivery to metabolic demand. The red blood cell (RBC) itself functions as an O(2) sensor, contributing to the control of O(2) delivery by releasing the vasodilators ATP and S-nitrosohaemoglobin with the offloading of O(2) from the haemoglobin molecule. Whether RBC number is sensed remains unknown. To investigate the role of RBC number, in isolation and in combination with alterations in blood oxygenation, on muscle and systemic perfusion, we measured local and central haemodynamics during one-legged knee-extensor exercise ( approximately 50% peak power) in 10 healthy males under conditions of normocythaemia (control), anaemia, anaemia + plasma volume expansion (PVX), anaemia + PVX + hypoxia, polycythaemia, polycythaemia + hyperoxia and polycythaemia + hypoxia, which changed either RBC count alone or both RBC count and oxyhaemoglobin. Leg blood flow (LBF), cardiac output (Q) and vascular conductance did not change with either anaemia or polycythaemia alone. However, LBF increased with anaemia + PVX (28 +/- 4%) and anaemia + PVX + hypoxia (46 +/- 6%) and decreased with polycythaemia + hyperoxia (18 +/- 5%). LBF and Q with anaemia + PVX + hypoxia (8.0 +/- 0.5 and 15.8 +/- 0.7 l min(-1), respectively) equalled those during maximal knee-extensor exercise. Collectively, LBF and vascular conductance were intimately related to leg arterial-venous (a-v) O(2) difference (r(2)= 0.89-0.93; P < 0.001), suggesting a pivotal role of blood O(2) gradients in muscle microcirculatory control. The systemic circulation accommodated to the changes in muscle perfusion. Our results indicate that, when coping with severe haematological challenges, local regulation of skeletal muscle blood flow and O(2) delivery primarily senses alterations in the oxygenation state of haemoglobin and, to a lesser extent, alterations in the number of RBCs and haemoglobin molecules.

  2. 全血白细胞计数危急值与临床的关系%Analysis of whole blood white blood cell count critical value and its clinical distribution

    Institute of Scientific and Technical Information of China (English)

    管学平; 陈鑫; 耿波; 左超

    2013-01-01

    目的 探讨全血白细胞计数危急值与临床的关系.方法 统计2009年1月至2011年1月期间本院医学检验部检测到的所有危急值,并通过查阅病历回顾性分析全血白细胞计数危急值患者的相关临床资料,分析其特点及分布规律.结果 共报告危急值2865个,其中全血白细胞危急值156个,共报告全血白细胞计数192960个测试,报出率为0.08%,占所有报告危急值的5.44%.高危急值病例主要在急性感染患者多见,其次为白血病、急性出血、恶性肿瘤、大手术后、烧伤以及肾功能衰竭患者;低危急值病例主要在白血病患者多见,其次为化疗后的恶性肿瘤患者.结论 全血白细胞计数危急值上下限的确定直接影响了全血白细胞计数危急值的报出率,应科学合理地设置危急值范围,分析其特点及疾病的分布规律,以便更好地服务于临床.%Objective To investigate the relationship between the critical value of the whole blood white blood cell count and the clinic.Methods The critical value of the whole blood white blood cell count from January 2009 to January 2011 in our hospital and its distribution were analyzed through medical records and patient clinical data.Results A total of 2 865 critical values were reported,of which 156 were critical value of the whole blood white blood cell count,reporting a total of 192 960 whole blood white blood cell count,with the report rate of 0.08%,accounting for 5.44% of all the reports of critical value.The number of critical value of the whole blood white blood cell count up upper limit was 101 (64.74%) and under lower limit was 55 (35.26%).Cases of high critical value occurred primary in patients with severe acute infection,followed by patients with leukemia,acute hemorrhagic,after major surgery,burn,cancer and renal failure patients.Cases of low critical value occurred primary in patients with leukemia,followed by patients with malignant tumors after

  3. Associations of White Blood Cell Count,Alanine Aminotransferase,and Aspartate Aminotransferase in the First Trimester withGestational Diabetes Mellitus.

    Science.gov (United States)

    2016-06-10

    Objective To explore the associations of white blood cell (WBC) count,alanine aminotransferase (ALT),and aspartate aminotransferase(AST) in the first trimester of pregnancy with gestational diabetes mellitus (GDM). Methods Totally 725 GDM women and 935 women who remained euglycemic throughout pregnancy were enrolled in this study. Pre-pregnancy weight/height were recorded. WBC,ALT,and AST levels were detected between 8 and 12 weeks of pregnancy.At 24 to 28 weeks of pregnancy,the glucose and insulin levels were measured. The WBC,ALT,and AST levels were compared between two groups,and the associations of WBC,ALT,and AST levels with the blood glucose and insulin levels were retrospectively analyzed. Meanwhile,the potential associations of those factors with the occurrence of GDM were analzyed. Results WBC count [9.41(8.15,10.84)?10(9)/L vs. 9.04 (7.64,10.37)?10(9)/L,P=1.0?10(-5)] and ALT levels [18.00(12.00,30.00)U/L vs. 16.00 (11.00,26.00)U/L,P=0.004] in the first trimester of pregnancy were significantly increased in GDM subjects than in normal glucose tolerance(NGT)subjects;however,the AST level showed no significant difference between these two groups [41.00 (26.00,43.00)U/L vs. 41.00 (23.00,43.00)U/L,P=0.588]. Logistic regression analysis illustrated that elevated WBC count was an independent risk factor for GDM after adjustment for age,pre-pregnancy body mass index,blood pressure,and family history of diabetes(OR=1.119,P=0.001). The ROC curve revealed that threshold of WBC count was 7.965?10(9)/L(AUC=0.566,P=1?10(-5)),which had a sensitivity of 79.4% and a specificity of 31.3%. Multivariate linear regression analysis showed that homeostasis model assessment of insulin resistance was positively correlated with WBC count(B=0.051,P=0.022,R(2)=0.083);1-hour blood glucose after oral 50 grams of sugar (B=0.044,P=0.001,R(2)=0.044) and fasting plasma true insulin(B=0.214,P=0.032,R(2)=0.066) were positively correlated with WBC count;1-hour true insulin after 100 grams

  4. Comparison of platelet clumping and complete blood count results with Sysmex XT-2000iV in feline blood sampled on EDTA or EDTA plus CTAD (citrate, theophylline, adenosine and dipyridamole).

    Science.gov (United States)

    Granat, Fanny; Geffré, Anne; Braun, Jean-Pierre; Trumel, Catherine

    2011-12-01

    False thrombocytopenia may result from platelet aggregation, especially in feline ethylenediamine tetra-acetic acid (EDTA) blood specimens. Citrate, theophylline, adenosine and dipyridamole (CTAD) was added to 46 feline EDTA specimens to test its anti-aggregation action. Platelet aggregation was estimated from blood films and a complete blood count was performed with a Sysmex XT-2000iV analyser. Platelet aggregation score was >2 in 11/46 EDTA tubes and only in one EDTA+CTAD specimen. The platelet count was higher in all CTAD-supplemented tubes except one, medians measured by cytometry being 225.5 × 10(9)/l and 249.0 × 10(9)/l in EDTA and EDTA+CTAD, respectively (P = 0.007). Adding CTAD had statistically and analytically significant but moderate effects on other blood variables, the most intense variations being observed for reticulocytes (about 3% higher in EDTA specimens) and reticulocyte indexes. Addition of CTAD to EDTA when sampling feline blood is a useful option to reduce platelet clumping.

  5. Association between Resting Heart Rate and Inflammatory Markers (White Blood Cell Count and High-Sensitivity C-Reactive Protein) in Healthy Korean People

    Science.gov (United States)

    Park, Woo-Chul; Seo, Inho; Kim, Shin-Hye

    2017-01-01

    Background Inflammation is an important underlying mechanism in the pathogenesis of atherosclerosis, and an elevated resting heart rate underlies the process of atherosclerotic plaque formation. We hypothesized an association between resting heart rate and subclinical inflammation. Methods Resting heart rate was recorded at baseline in the KoGES-ARIRANG (Korean Genome and Epidemiology Study on Atherosclerosis Risk of Rural Areas in the Korean General Population) cohort study, and was then divided into quartiles. Subclinical inflammation was measured by white blood cell count and high-sensitivity C-reactive protein. We used progressively adjusted regression models with terms for muscle mass, body fat proportion, and adiponectin in the fully adjusted models. We examined inflammatory markers as both continuous and categorical variables, using the clinical cut point of the highest quartile of white blood cell count (≥7,900/mm3) and ≥3 mg/dL for high-sensitivity C-reactive protein. Results Participants had a mean age of 56.3±8.1 years and a mean resting heart rate of 71.4±10.7 beats/min; 39.1% were men. In a fully adjusted model, an increased resting heart rate was significantly associated with a higher white blood cell count and higher levels of high-sensitivity C-reactive protein in both continuous (P for trend <0.001) and categorical (P for trend <0.001) models. Conclusion An increased resting heart rate is associated with a higher level of subclinical inflammation among healthy Korean people.

  6. [The changes in complete blood count in patients treated with sunitinib malate for metastatic clear cell renal cell carcinoma].

    Science.gov (United States)

    Kucharz, Jakub; Michałowska-Kaczmarczyk, Anna; Streb, Joanna; Kuzniewski, Marek; Herman, Roman M; Krzemieniecki, Krzysztof

    2013-01-01

    Renal cell carcinoma (RCC) accounts for approximately 3% of adult malignancies. For stage I - III RCC surgery is the primary treatment. Systemic therapy is used in the patients with disseminated disease (stage IV). Sunitinib malate is commonly used in the patients with clear cell renal cell carcinoma (ccRCC) rated as 'low' or 'intermediate' risk according to the Motzer scale. Treatment with sunitinib malate is associated with myelotoxicity. To assess its clinical significance we conducted a pilot study in a group of 10 patients. We noticed a gradual decrease in the mean haemoglobin level during subsequent treatment cycles. Alternations in the platelet count were of no clinical significance. Episodes of the neutropenia were noticed in the study group. In some patients neutrophil count decreased to the level that put them at risk of the infectious complications.

  7. High white blood cell count is associated with a worsening of insulin sensitivity and predicts the development of type 2 diabetes

    DEFF Research Database (Denmark)

    Vozarova, Barbora; Weyer, Christian; Lindsay, Robert S;

    2002-01-01

    Chronic low-grade inflammation may be involved in the pathogenesis of insulin resistance and type 2 diabetes. We examined whether a high white blood cell count (WBC), a marker of inflammation, predicts a worsening of insulin action, insulin secretory function, and the development of type 2 diabetes...... in Pima Indians. We measured WBC in 352 nondiabetic Pima Indians (215 men and 137 women, aged 27 +/- 6 years [means +/- SD], body fat 32 +/- 8%, WBC 8,107 +/- 2,022 cells/mm(3)) who were characterized for body composition (by hydrodensitometry or dual-energy X-ray absorptiometry), glucose tolerance (by 75...

  8. Determination of blood counts at sideropenic anemia, and monitoring depending on the outcome rate of iron absorption

    OpenAIRE

    PANOCHOVÁ, Daniela

    2011-01-01

    Sideropenic anemia (iron deficiency anemia) is the most common anemia all over the world. The cause of this anemia type is lack of iron. This thesis deals with the determination of blood picture treatment results and iron absorption in patients with sideropenic anemia. In the practical part, the methods of blood picture determination as well as iron determination and the resorption curve are described. The blood picture is provided at the Clinical Haematology Ward of the České Budějovice Hosp...

  9. 红细胞分布宽度和白细胞水平与冠心病的关系%Relationship of red blood cell distribution width and white blood cell count with coronary heart disease

    Institute of Scientific and Technical Information of China (English)

    孙洁静; 刘德平; 李华; 李辉; 张妮

    2015-01-01

    目的 观察红细胞分布宽度和白细胞水平与冠心病及冠状动脉病变的关系. 方法 选择行冠状动脉造影术患者590例,根据冠状动脉造影术结果分为冠心病组383例和对照组207例,并按冠状动脉病变支数将冠心病组分为不同的亚组,计算冠状动脉病变Gensini评分,比较各组间红细胞分布宽度和白细胞水平的差异,以及分析其与冠状动脉病变程度的相关性. 结果 冠心病组红细胞分布宽度和白细胞水平分别为(13.06±0.57)%、(6.33±1.56)×109/L,明显高于对照组(12.63±0.49)%、(5.86±1.29)×109/L(t=9.771、3.728,均P=0.000),并随着冠状动脉病变支数的增加而升高(F=51.454、7.544,均P=0.000),且与冠状动脉Gensini评分呈正相关(r=0.414、0.111,P=0.000、0.030);红细胞分布宽度与白细胞水平呈正相关(r=0.108,P=0.009).红细胞分布宽度对冠心病的预测价值进行受试者工作特征曲线(ROC)分析结果显示,红细胞分布宽度的界值为12.75%,ROC曲线下面积为0.723(95% CI:0.680~0.765),诊断的敏感度为67.6%,特异度为65.2%. 结论 红细胞分布宽度和白细胞水平在冠心病患者中明显升高,且与冠状动脉严重程度呈正相关,是冠心病的独立预测因子.%Objective To observe the relationship of the red blood cell distribution width and white blood cell count with coronary heart disease and coronary artery lesions.Methods Totally 590 patients undergoing coronary angiography were selected and divided into two groups based on the results of coronary angiography:coronary heart disease group (n=383) and control group (n=207).Based on the number of coronary lesions,patients in coronary heart disease were divided into different subgroup.The Gensini scores of coronary lesions were assessed.The differences in red blood cell distribution and white blood cell count were compared among different groups,and the correlations of coronary lesions with red blood cell distribution

  10. Role of enhanced glomerular synthesis of thromboxane A2 in progressive kidney disease.

    Science.gov (United States)

    Salvati, P; Ferti, C; Ferrario, R G; Lamberti, E; Duzzi, L; Bianchi, G; Remuzzi, G; Perico, N; Benigni, A; Braidotti, P

    1990-09-01

    Normotensive rats of the Milan strain (MNS) spontaneously develop focal glomerulosclerosis. In order to explore the contribution of glomerular thromboxane (TX) A2 synthesis to the development of the disease, we have characterized the time course of renal functional and biochemical changes, and their modification by long-term treatment with a TX-synthase inhibitor. Oral administration (150 mg.kg-1 from 1 to 14 months of age) of FCE 22178 suppressed enhanced glomerular TXB2 production at all experimental times (mean inhibition 80%) and proteinuria (varying between 27.1 and 73.0%) while preserving renal blood flow and glomerular filtration rate. These effects of TX-synthase inhibition were seen in the absence of any statistically significant changes in systemic blood pressure. Moreover, FCE 22178 had no antihypertensive effects in hypertensive rats of the Milan strain (MHS) nor in spontaneously hypertensive rats (SHR). Treatment also prevented the age-related hypoalbuminemia and hyperlipidemia observed in control MNS and significantly (P less than 0.01) reduced glomerular histologic damage, as demonstrated by light microscopy studies and measurement of sclerotic area. We conclude that: 1) MNS rats provide an animal model of long-lasting proteinuria characterized by an age-related increase in glomerular TXB2 production paralleled by progressive loss of renal structural integrity and function and by a secondary dyslipidemia; 2) pharmacological inhibition of glomerular TX-synthase attenuates the structural as well as the functional expression of kidney disease, without a primary effect on systemic blood pressure. These data are suggestive of an important modulating role of TXA2 in the progression of MNS renal disease.

  11. The Effect of 8 Weeks Endurance Training on Serum Levels of IL-10 and IL-8, and White Blood Cell Count in Women with Breast Cancer

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    Abdolreza Kazemi

    2016-09-01

    Full Text Available Background & Objective: The regular exercise training is known as a preventive and adjuvant therapy in inflammatory diseases such as cancer. The aim of this study was to determine the effect of the aerobic training on IL-10 and IL-8 levels and the count of white blood cells (WBC in women with breast cancer. Material & Methods: The statistical society of the present study included Kerman women with breast cancer. Forty patients with breast cancer were randomly divided into two groups: exercise (n = 20 and control (n = 20. The exercise group performed the endurance training for 8 weeks with the intensity between 40 to 55 percent of the target heart rate. Twenty four hours before the first session and 48 hours after the last session of the exercise protocol, blood samples were taken from both groups and then IL-10 and IL-8 levels in serum were measured by ELISA via a Boster kit. Results: The results of the present study showed that 8 weeks of the endurance exercise training did not significantly increase the IL-10 (P=0.113 serum level, but increased the WBC count (p=0.019 and decreased the serum level of IL-8 (p=0.03 significantly. Conclusion: According to the results of the present study, regular endurance training via decreasing the inflammatory factors can be considered as an effective factor along with other therapies in improving breast cancer.

  12. Hypersensitive prostaglandin and thromboxane response to hormones in rabbit colitis

    Energy Technology Data Exchange (ETDEWEB)

    Zipser, R.D.; Patterson, J.B.; Kao, H.W.; Hauser, C.J.; Locke, R.

    1985-10-01

    Inflammation of the colon is associated with increased production of prostaglandins (PG) and thromboxanes (Tx), and these eicosanoids may contribute to the inflammatory, secretory, and motility dysfunctions in colitis. To evaluate the potential role of peptide hormones in the enhanced eicosanoid release, colitis was established in rabbits by a delayed-type hypersensitivity reaction to dinitrochlorobenzene and by an immune-complex-mediated reaction. PG and Tx were identified in the venous effluent of isolated perfused colons by radiochromatography after ( UC)arachidonic acid prelabeling, as well as by bioassay, and then quantitated by immunoassay. The two colitis models were morphologically similar. Basal release of PGE2, PGI2, and TxA2 was two- to threefold greater from colitis tissue than from control tissue. Bradykinin (BK) and angiotensin II (ANG II) increased release of UC-labeled eicosanoids, whereas several gastrointestinal hormones had no effect. In control colons, BK and ANG II increased PGE2 and PGI2 release (by about 2-fold) but did not alter TxA2. In contrast, BK and ANG II markedly exaggerated the release of eicosanoids in colitis. Since BK and possibly ANG II are increased at sites of inflammation, the hypersensitive eicosanoid response to these peptides may augment the eicosanoid-mediated manifestations of colitis.

  13. Correlation of the severity of atopic dermatitis with absolute eosinophil counts in peripheral blood and serum IgE levels

    Directory of Open Access Journals (Sweden)

    Dhar Sandipan

    2005-01-01

    Full Text Available BACKGROUND: Although a number of epidemiological studies, showing incidence and prevalence of atopic dermatitis, were available, scant attention has been paid to the correlation between the parameters of the disease like severity, absolute eosinophil count and IgE level, which has been known to be associated inconsistently. Hence this study was undertaken. METHODS: A total of 102 patients of atopic dermatitis, both children and adults, and 107 age matched controls were studied at the Pediatric Dermatology clinic, Institute of Child Health and department of Dermatology, AMRI-Apollo hospitals, Kolkata. RESULTS: The average age of onset of atopic dermatitis was observed to be 4.55 years. Both the average absolute eosinophil count and IgE levels in patients of atopic dermatitis were significantly higher than that of the controls. Each of these parameters showed significant correlation with severity of the disease and showed a nonhomogeneous distribution reflected by significant association with personal history of bronchial asthma and family history of atopy, when both parents were atopic. CONCLUSIONS: Our study shows that clinical activity of the disease as recorded by the "SCORAD" index can be used as an indicator of the hematological abnormalities as well as to some extent as a prognostic indicator. Family history of atopy correlates with the hematological abnormalities only if both parents are involved and bronchial asthma is the only associated atopic condition which correlates with the parameters of the disease .

  14. Radioactive 131Iodine Body Burden and Blood Dose Estimates in Treatment for Differentiated Thyroid Cancer by External Probe Counting

    Science.gov (United States)

    Ravichandran, Ramamoorthy; Al Balushi, Naima

    2016-01-01

    Radioactive131 iodine (RAI) body burden is estimated in thyroid cancer patients by a) exposure rate meter and b) external probe counting. A calibration factor of 301 cpm/MBq (d = 16 cm) is used for the probe for estimates of whole body activity. Patients sit in a rotating stool with their center corresponding to the field of view for estimation of whole body RAI. Radioactive counts are obtained for anterio posterior (AP) and postero anterior (PA) geometries. Whole body retention factor is expressed as a ratio against assayed activity administered to the patient on day 1. With exposure rate measurement, for off-thyroxin (hypothyriod) patients, the retention factors were 0.148 ± 0.12 (n = 211) and 0.07 ± 0.08 (n = 68) at 48 h and 72 h, respectively. For recombinant TSH (rhTSH) (euthyroid) group, the retention factors were 0.089 ± 0.06 (n = 24) and 0.05 ± 0.05 (n = 19) at 48 h and 72 h, respectively. By probe counting method, the obtained retention factors were 0.081 ± 0.013 (range: 0.071–0.096) (off-thyroxine group) and 0.039 ± 0.03 (range: 0.008–0.089) for the rhTSH group at 48 h. The 72 h retentions in the off thyroxine (hypothyriod) group and the rhTSH (euthyroid) group were 0.048 ± 0.024 (range: 0.016–0.076) and 0.005, respectively. The radioactive body burdens at 48 h were in the range of 290-315 MBq (7.8–8.5 mCi) for the off-thyroxine group and 44–286 MBq (1.2–7.7 mCi) for the rhTSH group. The calculated residence times in whole body were 21.97 ± 3.8 h (range: 17.1–27.1) for off-thyroxine group and 14.28 ± 2.75 h (range: 9.97-19.46) showing high statistical significance (P radioactive iodine. There was no correlation between administered activity and retained body burden at 48 h or 72 h. PMID:27651735

  15. A rare IL33 loss-of-function mutation reduces blood eosinophil counts and protects from asthma

    Science.gov (United States)

    Sulem, Patrick; Bjornsdottir, Unnur Steina; Lim, Ai Ching; Sveinbjornsson, Gardar; Brown, Michael; Garrett, Logan; Jonasdottir, Adalbjorg; Jonasdottir, Aslaug; Sigurdsson, Asgeir; Magnusson, Olafur T.; Eyjolfsson, Gudmundur I.; Olafsson, Isleifur; Onundarson, Pall Torfi; Sigurdardottir, Olof; Gislason, David; Gislason, Thorarinn; Ludviksson, Bjorn Runar; Ludviksdottir, Dora; Boezen, H. Marike; Heinzmann, Andrea; Porsbjerg, Celeste; Waage, Johannes; Backer, Vibeke; Deichmann, Klaus A.; Bønnelykke, Klaus; Bisgaard, Hans; Gudbjartsson, Daniel F.; Johnston, James A.; Jonsdottir, Ingileif; Stefansson, Kari

    2017-01-01

    IL-33 is a tissue-derived cytokine that induces and amplifies eosinophilic inflammation and has emerged as a promising new drug target for asthma and allergic disease. Common variants at IL33 and IL1RL1, encoding the IL-33 receptor ST2, associate with eosinophil counts and asthma. Through whole-genome sequencing and imputation into the Icelandic population, we found a rare variant in IL33 (NM_001199640:exon7:c.487-1G>C (rs146597587-C), allele frequency = 0.65%) that disrupts a canonical splice acceptor site before the last coding exon. It is also found at low frequency in European populations. rs146597587-C associates with lower eosinophil counts (β = -0.21 SD, P = 2.5×10–16, N = 103,104), and reduced risk of asthma in Europeans (OR = 0.47; 95%CI: 0.32, 0.70, P = 1.8×10–4, N cases = 6,465, N controls = 302,977). Heterozygotes have about 40% lower total IL33 mRNA expression than non-carriers and allele-specific analysis based on RNA sequencing and phased genotypes shows that only 20% of the total expression is from the mutated chromosome. In half of those transcripts the mutation causes retention of the last intron, predicted to result in a premature stop codon that leads to truncation of 66 amino acids. The truncated IL-33 has normal intracellular localization but neither binds IL-33R/ST2 nor activates ST2-expressing cells. Together these data demonstrate that rs146597587-C is a loss of function mutation and support the hypothesis that IL-33 haploinsufficiency protects against asthma. PMID:28273074

  16. The influences of Chan-Chuang qi-gong therapy on complete blood cell counts in breast cancer patients treated with chemotherapy.

    Science.gov (United States)

    Yeh, Mei-Ling; Lee, Tsoy-Ing; Chen, Hsing-Hsia; Chao, Tsu-Yi

    2006-01-01

    After surgery, breast cancer patients are offered adjuvant chemotherapy to avoid cancer cell spread. During chemotherapy process, neutrophils could fall relatively, and side effects could spike to the peak. Therefore, the medical care personnel should prevent the progression of the side effects. This study aimed to examine the effects of Chan-Chuang qi-gong therapy on complete blood counts in breast cancer patients treated with chemotherapy. This study used a quasi-experimental design. The experiment group (n = 32) received a 21-day Chan-Chuang qi-gong therapy, whereas the control group (n = 35) did not. White blood cells, platelet, and hemoglobin were measured on the day before chemotherapy and on days 8, 15, and 22 during chemotherapy. According to this study, there were significant differences in white blood cells (F = 115.76, P qi-gong therapy may decrease leukopenia in breast cancer patients treated with chemotherapy. It is recommended conducting more studies on qi-gong and then introducing it in clinical nursing practice at an appropriate time to promote quality of nursing care and quality of patient life.

  17. Thromboxane synthase deficiency improves insulin action and attenuates adipose tissue fibrosis

    Science.gov (United States)

    Lei, Xia; Li, Qing; Rodriguez, Susana; Tan, Stefanie Y.; Seldin, Marcus M.; McLenithan, John C.; Jia, Weiping

    2015-01-01

    Thromboxane A2, an arachidonic acid-derived eicosanoid generated by thromboxane synthase (TBXAS), plays critical roles in hemostasis and inflammation. However, the contribution of thromboxane A2 to obesity-linked metabolic dysfunction remains incompletely understood. Here, we used in vitro and mouse models to better define the role of TBXAS in metabolic homeostasis. We found that adipose expression of Tbxas and thromboxane A2 receptor (Tbxa2r) was significantly upregulated in genetic and dietary mouse models of obesity and diabetes. Expression of Tbxas and Tbxa2r was detected in adipose stromal cells, including macrophages. Furthermore, stimulation of macrophages with interferon-γ or resistin factors known to be upregulated in obesity induced Tbxas and Tbxa2r expression. Mice lacking Tbxas had similar weight gain, food intake, and energy expenditure. However, loss of Tbxas markedly enhanced insulin sensitivity in mice fed a low-fat diet. Improvement in glucose homeostasis was correlated with the upregulated expression of multiple secreted metabolic regulators (Ctrp3, Ctrp9, and Ctrp12) in the visceral fat depot. Following a challenge with a high-fat diet, Tbxas deficiency led to attenuated adipose tissue fibrosis and reduced circulating IL-6 levels without adipose tissue macrophages being affected; however, these changes were not sufficient to improve whole body insulin action. Together, our results highlight a novel, diet-dependent role for thromboxane A2 in modulating peripheral tissue insulin sensitivity and adipose tissue fibrosis. PMID:25738781

  18. Thromboxane A2 release in ischemia and reperfusion of free flaps in rats, studied by microdialysis.

    Science.gov (United States)

    Ionac, M; Schaefer, D; Geishauser, M

    2001-02-01

    Several studies have implicated enhanced eicosanoid production in reperfusion injury. The reported study investigated the use of microdialysis in the in vivo measurement of thromboxane levels during reperfusion in ischemic and reperfused experimental free muscle flaps. Microdialysis probes were inserted, via a guide, into the gracilis and semitendinosus free flap in the rat. The probe was perfused at a flow of 5 microl/min, with samples collected at intervals of 20 min, and analyzed by the ELISA technique. Animals were randomly distributed into three groups. After ischemic periods of 2, 4, and 6 hr, respectively, the free muscle flaps were revascularized on the contralateral femoral vessels. The mean thromboxane level during ischemia was 1785.34 +/- 124.81 pg/ml. The mean levels of thromboxane rose significantly (p ischemia group, 192.33 percent in the 4-hr ischemia group, and 294.69 percent in the 6-hr ischemia group, and correlated well with histologic observations. The results suggest that a microdialysis technique, combined with a sensitive assay for measuring thromboxane, is a useful method for in vivo monitoring of inflammatory processes during ischemia and reperfusion. The evolution of thromboxane release following 6 hr of ischemia indicates that this mediator may be involved in facilitation of cell death, following ischemia and reperfusion, since its tissue level correlates with histologic tissue damage.

  19. Counting carbohydrates

    Science.gov (United States)

    Carb counting; Carbohydrate-controlled diet; Diabetic diet; Diabetes-counting carbohydrates ... Many foods contain carbohydrates (carbs), including: Fruit and fruit juice Cereal, bread, pasta, and rice Milk and milk products, soy milk Beans, legumes, ...

  20. Platelet Count

    Science.gov (United States)

    ... their spleen removed surgically Use of birth control pills (oral contraceptives) Some conditions may cause a temporary (transitory) increased ... increased platelet counts include estrogen and birth control pills (oral contraceptives). Mildly decreased platelet counts may be seen in ...

  1. Seal Counts

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Database of seal counts from aerial photography. Counts by image, site, species, and date are stored in the database along with information on entanglements and...

  2. Dynamics of C-reactive protein and white blood cell count in critically ill patients with nosocomial Gram positive vs. Gram negative bacteremia: a historical cohort study

    Directory of Open Access Journals (Sweden)

    Depuydt Pieter O

    2007-09-01

    Full Text Available Abstract Background Nosocomial bacteremia is associated with a poor prognosis. Early adequate therapy has been shown to improve outcome. Consequently, rapid detection of a beginning sepsis is therefore of the utmost importance. This historical cohort study was designed to evaluate if different patterns can be observed in either C-reactive protein (CRP and white blood cell count (WCC between Gram positive bacteremia (GPB vs. Gram negative bacteremia (GNB, and to assess the potential benefit of serial measurements of both biomarkers in terms of early antimicrobial therapy initiation. Methods A historical study (2003–2004 was conducted, including all adult intensive care unit patients with a nosocomial bacteremia. CRP and WCC count measurements were recorded daily from two days prior (d-2 until one day after onset of bacteremia (d+1. Delta (Δ CRP and Δ WCC levels from the level at d-2 onward were calculated. Results CRP levels and WCC counts were substantially higher in patients with GNB. Logistic regression analysis demonstrated that GNB and Acute Physiology and Chronic Health Evaluation (APACHE II score were independently associated with a CRP increase of 5 mg/dL from d-2 to d+1, and both were also independently associated with an increase of WCC levels from d-2 to d+1 of 5,000 × 103 cells/mm3. Conclusion Increased levels of CRP and WCC are suggestive for GNB, while almost unchanged CRP and WCC levels are observed in patients with GPB. However, despite the different patterns observed, antimicrobial treatment as such cannot be guided based on both biomarkers.

  3. Use of matrix metalloproteinases 2 and 9 and white blood cell counts in monitoring the treatment and predicting the survival of horses with septic arthritis.

    Science.gov (United States)

    Kidd, J A; Barr, A R S; Tarlton, J F

    2007-09-01

    Thirty-nine samples of synovial fluid were collected from the joints of 32 horses with suspected septic arthritis and 39 samples were collected from horses euthanased for non-orthopaedic conditions. The white blood cell counts (WBCC) were determined and the pro and active forms of matrix metalloproteinases (MMPs) 2 and 9 were measured by gelatin zymography and image analysis in each sample. The initial measurements of the ratio of proMMP9:proMMp2 and WBCC were good prognostic indicators of the survival of the horses. There was no significant relationship between the interval between the injury and the horse being referred for treatment and either the WBCC or the levels of MMP2 and MMP9 initially, and no evidence that this interval significantly affected the chances of the horses surviving.

  4. Effects of Some Plant Extracts in Triticale-Based Broiler Diets on Growth Performance, E.Coli Counts in Intestine and Blood Parameters

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    Ergun Demir

    2016-10-01

    Full Text Available This study was conducted to determine the effect of using plant essential oils in triticale-based broiler diets as alternative to antimicrobial growth promotors (AGPs on growth, E.coli counts in small intestine and blood parameters. 720 male broiler chicks were randomly distributed to nine experimental groups with four replicates. The control group received the triticale-soybean meal basal diet. In the other treatment groups the basal diet was supplemented with one of the following; flavomycin, enzyme, flavomycin+enzyme, enzyme+thyme oil, enzyme+fennel oil, enzyme+bay leaf oil, enzyme+thyme oil+fennel oil and enzyme+thyme oil+fennel oil+bay leaf oil. Results showed that the weight gain and feed intake were not influenced by the treatments (P>0,05. The use of the combination of plant extracts significantly improved the feed conversion. Use of plant extracts in triticale-based broiler diets as an alternative to AGPs had no regular effect on E.coli count in small intestine segments (P>0,05. Feeding broiler chickens with the enzyme+thyme oil supplemented diets increased eritrocyte level in serum (P0,05 by the treatments. In conclusion, these plant ectracts can be also used with enzyme in triticale-based broiler diets as alternative to AGPs.

  5. Concentrations of procalcitonin and C-reactive protein, white blood cell count, and the immature-to-total neutrophil ratio in the blood of neonates with nosocomial infections: Gram-negative bacilli vs coagulase-negative staphylococci.

    Science.gov (United States)

    Kordek, A

    2011-03-01

    This study was undertaken to determine whether concentrations of procalcitonin in the blood of neonates with nosocomial infections depend on the type of pathogen. Qualification for the study group was based on the clinical signs of infection. We found that infections with Gram-positive (chiefly coagulase-negative staphylococci) and Gram-negative bacteria are accompanied by elevated concentrations of procalcitonin. In the case of Gram-positive bacteria, other laboratory signs of infection studied by us (concentration of C-reactive protein, white blood cell count, immature-to-total neutrophil ratio) were not discriminatory, confirming the diagnostic usefulness of procalcitonin measurements in nosocomial infections of the neonate with Gram-negative or Gram-positive bacteria.

  6. 血细胞自动化分析后血涂片复审标准制定的原则与步骤%Principles and procedure of determining criteria for smear review following automated complete blood count and leukocyte differential count

    Institute of Scientific and Technical Information of China (English)

    丛玉隆; 王昌富; 乐家新

    2008-01-01

    根据血细胞分析方法的变迁和仪器法血细胞检测后血涂片复审的现状,结合血涂片复审41条国际规则,介绍临床实验室制定血涂片复审标准的原则和步骤,以及值得注意的问题.%The history of automated blood cell analysis and current situation regarding the peripheral blood smear review following automated complete blood count(CBC) and leukocyte differential count(LDC) was introduced. Principles and procedure of determining criteria for blood smear review in clinical laboratory was elucidated in combination with 41 consensus rules for the review of automated CBC and LDC proposed by the International Consensus Group for blood smear review.

  7. In vitro effects of mercury on platelet aggregation, thromboxane and vascular prostacyclin production.

    Science.gov (United States)

    Caprino, L; Togna, A R; Cebo, B; Dolci, N; Togna, G

    1983-01-01

    Mercuric chloride [25-50 micrograms/ml platelet-rich plasma (PRP)] lowers the threshold concentration of arachidonic acid (AA) required for triggering rabbit platelet aggregation and causes a marked increase of thromboxane production. The metal, added as HgCl2, does not modify (50 micrograms/ml PRP) or block (100 micrograms/ml PRP) the platelet aggregation wave induced by a normal aggregating dose of AA. Whether or not AA-induced platelet aggregation takes place, a large increase in thromboxane production is observed. Methyl mercury, assayed as reference drug, induces platelet aggregation and a significant increase of thromboxane levels. Finally, HgCl2 and methyl mercury, in a concentration range of 0.125-0.5 micrograms/microliters in the incubation liquid, induce an increased prostacyclin release from rat aortic tissue.

  8. Natural functional SNPs in miR-155 alter its expression level, blood cell counts and immune responses

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    Congcong Li

    2016-08-01

    Full Text Available miR-155 has been confirmed to be a key factor in immune responses in humans and other mammals. Therefore, investigation of variations in miR-155 could be useful for understanding the differences in immunity between individuals. In this study, four SNPs in miR-155 were identified in mice (Mus musculus and humans (Homo sapiens. In mice, the four SNPs were closely linked and formed two miR-155 haplotypes (A and B. Ten distinct types of blood parameters were associated with miR-155 expression under normal conditions. Additionally, 4 and 14 blood parameters were significantly different between these two genotypes under normal and lipopolysaccharide (LPS stimulation conditions, respectively. Moreover, the expression levels of miR-155, the inflammatory response to LPS stimulation and the lethal ratio following Salmonella typhimurium infection were significantly increased in mice harboring the AA genotype. Further, two SNPs, one in the loop region and the other near the 3' terminal of pre-miR-155, were confirmed to be responsible for the differential expression of miR-155 in mice. Interestingly, two additional SNPs, one in the loop region and the other in the middle of miR-155*, modulated the function of miR-155 in humans. Predictions of secondary RNA structure using RNAfold showed that these SNPs affected the structure of miR-155 in both mice and humans. Our results provide novel evidence of the natural functional SNPs of miR-155 in both mice and humans, which may affect the expression levels of mature miR-155 by modulating its secondary structure. The SNPs of human miR-155 may be considered as causal mutations for some immune-related diseases in the clinic. The two genotypes of mice could be used as natural models for studying the mechanisms of immune diseases caused by abnormal expression of miR-155 in humans.

  9. Genome-wide association study of white blood cell count in 16,388 African Americans: the continental origins and genetic epidemiology network (COGENT.

    Directory of Open Access Journals (Sweden)

    Alexander P Reiner

    2011-06-01

    Full Text Available Total white blood cell (WBC and neutrophil counts are lower among individuals of African descent due to the common African-derived "null" variant of the Duffy Antigen Receptor for Chemokines (DARC gene. Additional common genetic polymorphisms were recently associated with total WBC and WBC sub-type levels in European and Japanese populations. No additional loci that account for WBC variability have been identified in African Americans. In order to address this, we performed a large genome-wide association study (GWAS of total WBC and cell subtype counts in 16,388 African-American participants from 7 population-based cohorts available in the Continental Origins and Genetic Epidemiology Network. In addition to the DARC locus on chromosome 1q23, we identified two other regions (chromosomes 4q13 and 16q22 associated with WBC in African Americans (P<2.5×10(-8. The lead SNP (rs9131 on chromosome 4q13 is located in the CXCL2 gene, which encodes a chemotactic cytokine for polymorphonuclear leukocytes. Independent evidence of the novel CXCL2 association with WBC was present in 3,551 Hispanic Americans, 14,767 Japanese, and 19,509 European Americans. The index SNP (rs12149261 on chromosome 16q22 associated with WBC count is located in a large inter-chromosomal segmental duplication encompassing part of the hydrocephalus inducing homolog (HYDIN gene. We demonstrate that the chromosome 16q22 association finding is most likely due to a genotyping artifact as a consequence of sequence similarity between duplicated regions on chromosomes 16q22 and 1q21. Among the WBC loci recently identified in European or Japanese populations, replication was observed in our African-American meta-analysis for rs445 of CDK6 on chromosome 7q21 and rs4065321 of PSMD3-CSF3 region on chromosome 17q21. In summary, the CXCL2, CDK6, and PSMD3-CSF3 regions are associated with WBC count in African American and other populations. We also demonstrate that large inter

  10. Influence of nutrient intake on antioxidant capacity, muscle damage and white blood cell count in female soccer players

    Directory of Open Access Journals (Sweden)

    Gravina Leyre

    2012-07-01

    Full Text Available Abstract Background Soccer is a form of exercise that induces inflammatory response, as well as an increase in free radicals potentially leading to muscle injury. Balanced nutritional intake provides important antioxidant vitamins, including vitamins A, C and E, which may assist in preventing exercise-related muscle damage. The purpose of the present study was to determine the effect of macro/micronutrient intake on markers of oxidative stress, muscle damage, inflammatory and immune response in female soccer players. Methods Twenty-eight female players belonging to two soccer teams of the same professional soccer club participated in this study after being informed about the aims and procedures and after delivering written consent. Each team completed an 8-day dietary record and played one competition match the same week. Participants were divided into two groups: the REC group (who complied with recommended intakes and the NO-REC group (who were not compliant. Laboratory blood tests were carried out to determine hematological, electrolytic and hormonal variables, as well as to monitor markers of cell damage and oxidative stress. Blood samples were obtained 24 h before, immediately after and 18 h after official soccer matches. Student t-test or Mann–Whitney U-test was used to compare both groups throughout the match. Results At rest, we observed that the REC group had higher levels of total antioxidant status (TAS, glutathione peroxidase (GPx, and lower levels of creatine kinase (CK and lactate dehydrogenase (LDH in comparison to the NO-REC group. Immediately after the match, levels of TAS, GPx, superoxide dismutase (SOD, LDH and % lymphocytes were higher and the % of neutrophils were lower in the REC group compared to the NO-REC group. These differences were also maintained 18 h post-match, only for TAS and GPx. Conclusions Our data reveal an association between nutritional intake and muscle damage, oxidative stress, immunity and inflammation

  11. Pharmacologic antagonism of thromboxane A2 receptors by trimetoquinol analogs in vitro and in vivo

    Energy Technology Data Exchange (ETDEWEB)

    Shin, Y.; Romstedt, K.J.; Doyle, K.; Harrold, M.W.; Gerhardt, M.A.; Miller, D.D.; Patil, P.N.; Feller, D.R. (Ohio State University, Columbus (USA))

    1991-01-01

    Although (-)-(S)-trimetoquinol (1-(3,4,5-trimethoxy-benzyl)- 6,7-dihydroxy-1,2,3,4-tetrahydroisoquinoline; TMQ) is recognized as a potent bronchodilator, (+)-(R)-TMQ is a selective antagonist of human platelet aggregation and serotonin secretion induced by thromboxane A2 (TXA2) agonists. To confirm the pharmacological actions of TMQ analogs, the interaction of the drugs with TXA2 receptors was examined in human platelets and in a mouse sudden death model. The inhibitory potencies of TMQ analogs (pIC50 values) for displacement of (3H)SQ 29,548 binding to platelets showed excellent correlation with the respective pIC50 (-log IC50) values for U46619-induced aggregation (r = 0.99, P less than 0.01) and serotonin secretion (r = 0.99, P less than 0.01) in human platelet-rich plasma and for whole blood aggregation (r = 0.99, P less than 0.01). In each system, the rank order of inhibitory potencies was rac-iodoTMQ greater than or equal to (+)-(R)-TMQ greater than rac-TMQ much greater than (-)-(S)-TMQ. Antithrombotic effects of TMQ analogs were evaluated in a mouse sudden death model. In vivo antithrombotic potencies of these compounds were consistent with the in vitro potencies as TXA2 receptor antagonists in platelet systems. Administration of rac-iodoTMQ, (+)-(R)-TMQ and rac-TMQ 15 min before the injection of U46619 (800 micrograms/kg, iv) protected mice against U46619-induced sudden death. On the other hand, (-)-(S)-TMQ did not protect animals against death. Protection of U46619-induced cardiopulmonary thrombosis by TMQ analogs was seen at doses of 3-100 mg/kg.

  12. Could mean platelet volume among complete blood count parameters be a surrogate marker of metabolic syndrome in pre-pubertal children?

    Science.gov (United States)

    Aypak, Cenk; Türedi, Ozlem; Bircan, Mustafa A; Yüce, Adnan

    2014-01-01

    Interest in childhood metabolic syndrome (MetS) has increased substantially due to the increasing prevalence of childhood obesity on a global scale. Early recognition of MetS is critical in order to delay the development of cardiovascular disease (CVD). In this study, we evaluated the relationship between complete blood count (CBC) parameters and MetS among pre-pubertal children which may provide evidence in support of using low cost, readily available clinical haematological parameters for the detection of MetS. A retrospective analysis was carried out on 330 (125 lean vs. 205 overweight) Turkish pre-pubertal children who attend to a paediatric outpatient clinic. Age, gender, puberty, body mass index, CBC parameters, cardiometabolic risk factors including lipid profiles, high sensitive serum reactive protein (hsCRP) and insulin resistance index calculated by homeostasis model assessment (HOMA-IR) were evaluated and compared among lean, overweight children and children with MetS. The mean age of the study population was 7.4 ± 1.9 years. In both gender, the mean values of mean corpuscular volume (MCV), mean corpuscular haemoglobin (MCH) and mean corpuscular haemoglobin concentration (MCHC) were significantly lower and red blood cell (RBC), platelet (PLT) counts were significantly higher in overweight children. Overall, 8.4% (n = 28) of patients met the criteria of MetS. Children with MetS had higher levels of PLT and lower levels of mean platelet volume (MPV). Of all the haematological parameters analysed, PLT was positively, whereas MPV was negatively correlated with MetS in girls. In addition, MPV was inversely correlated with fasting blood glucose, HOMA-IR, low density lipoprotein-cholesterol (LDL-C) and low density lipoprotein-cholesterol/high density lipoprotein-cholesterol (LDL-C/HDL-C) ratio in girls after adjusting for confounding factors. The risk analyses of MetS in terms of MPV quartiles showed that the adjusted OR (95% CI) for the lowest vs. the

  13. Diabetic microangiopathy and peripheral blood neutrophil count%糖尿病微血管病变与外周血中性粒细胞计数

    Institute of Scientific and Technical Information of China (English)

    王静; 何方平; 娜丽玛; 伊力哈木江·依马木

    2015-01-01

    BACKGROUND:Course of diabetes and hypertension are independent risk factors for diabetic microvascular complications, and fasting C-peptide level is a protective factor. There are many studies concerning glycated hemoglobin, C-reactive protein, white blood cel count and diabetic microvascular complications, but studies about neutrophils are rarely reported. OBJECTIVE:To investigate the relationship of peripheral blood neutrophil count with diabetic microvascular complications. METHODS:Totaly 112 patients with type 2 diabetes melitus were selected and divided into two groups according to the presence or absence of microvascular complications: diabetes group and diabetes+ microvascular complication group. The gender, age, ethnic group, disease history and laboratory examination parameters were colected and analyzed with univariate analysis and multivariate analysis. RESULTS AND CONCLUSION:(1) Univariate analysis: Patients in the diabetes+microvascular complication group had significantly higher age, course of diabetes, globulin, creatinine, the percentage of neutrophile granulocytes, nertrophil count, rate of hypertension than those in the diabetes group (P < 0.05), while the lymphocyte percentage, glomerular filtration rate, and triglyceride level were significantly lower than those in the diabetes group (P < 0.05). (2) Logistic regression analysis showed that age, course of diabetes and nertrophil count were the independent risk factors of microvascular complications in patients with type 2 diabetes melitus (odds ratio=1.155, 2.145, 2.275, alP < 0.01). Based on the adjustment of the age factor, the partial correlation analysis showed that the nertrophil count was positively correlated to the levels of creatinine, low-density lipoprotein cholesterol, globulin and hemoglobin, but negatively correlated to the levels of serum bile acid and glomerular filtration rate. Chronic subclinical inflammations are closely correlated to the occurrence of microvascular

  14. Evidence for homogeneity of thromboxane A2 receptor using structurally different antagonists.

    Science.gov (United States)

    Swayne, G T; Maguire, J; Dolan, J; Raval, P; Dane, G; Greener, M; Owen, D A

    1988-08-01

    Nine structurally dissimilar thromboxane antagonists (SQ 29548, ICI 185282, AH 23848, BM 13505 (Daltroban), BM 13177 (Sulotroban), SK&F 88046, L-636499, L-640035 and a Bayer compound SK&F 47821) were studied for activity as thromboxane A2 receptor antagonists. The assays used were inhibition of responses induced by the thromboxane mimetic, U46619, on human washed platelet aggregation, rabbit platelet aggregation, rabbit aortic strip contraction, anaesthetised guinea-pig bronchoconstriction, and a radio-labelled ligand (125I-PTA-OH) binding assay as a measure of affinity for the human platelet receptor. The results of the present study, with activities spanning at least four orders of magnitude along with statistically significant correlations (at least P less than 0.01), strongly suggests that between assays, antagonists and species a homogenous population of thromboxane A2 receptors exists. This finding is in contrast to those of a close series of 13-azapinane antagonists studied by other workers which have suggested receptor heterogeneity.

  15. 孕妇外周血中有核红细胞数与妊娠期高血压疾病的关系%To Predict Hypertention by Pregnancy in Counting Nuclear Red Blood Cells in Peripheral Blood

    Institute of Scientific and Technical Information of China (English)

    刘丽平; 龚瑞龙; 周洁琼; 戴翔; 宋晓婕

    2016-01-01

    目的:通过比较正常孕妇和妊娠期高血压疾病孕妇外周血中胎儿有核红细胞数量,了解孕妇外周血中胎儿有核红细胞数量变化与妊娠期高血压疾病之间的关系。方法:收集69例孕12~18周孕妇外周血,采用密度梯度离心法分离外周单个核细胞后使用 PE-GPA/FITC-CD71单克隆抗体标记胎儿有核红细胞后进行流式细胞计数,分析其与妊娠期高血压疾病之间的关联性。结果:妊娠期高血压疾病患者外周血中胎儿有核红细胞较正常妊娠者增多,对照组和病例组中 GPA+/CD71+百分比分别为(0.89±0.81)%和(3.57±3.48)%,其差异具有统计学意义(P <0.05)。结论:妊娠期高血压疾病患者外周血中胎儿有核红细胞增多,孕妇外周血中胎儿有核红细胞增加提示有患妊娠期高血压疾病的可能,应积极采取干预措施。%Objective To predict the Hypertention by pregnancy in counting nuclear red blood cells pe-ripheral blood. Methods Blood samples were obtained from 69 pregnant women with the gestational age from 12 to 18 weeks. Peripheral blood mononuclear cells were isolated by density gradient centrifugation and then analyzed the cells marked with PE-GPA/FITC-CD71 monoclonal antibodies on the flow cytometer. Correlations between them were analyzed after tracking of hypertention by pregnancy. Results Percentage of GPA+/CD71+ cells in healthy pregnant women was 0.89 ± 0.81%, while that in pregnant women with hypertention was 3.57 ± 3.48%. There was a significant difference between the two groups (P<0.05), and the percentage of GPA+/CD71+cells in the group of hypertention by pregnancy was much higher than that in the healthy group. Conclusions The increased nuclear red blood cells in maternal peripheral blood related to hypertention by pregnancy. The counting of nuclear red blood cells in maternal peripheral blood have potential value to predict hypertention by

  16. Multiplicity Counting

    Energy Technology Data Exchange (ETDEWEB)

    Geist, William H. [Los Alamos National Lab. (LANL), Los Alamos, NM (United States)

    2015-12-01

    This set of slides begins by giving background and a review of neutron counting; three attributes of a verification item are discussed: 240Pueff mass; α, the ratio of (α,n) neutrons to spontaneous fission neutrons; and leakage multiplication. It then takes up neutron detector systems – theory & concepts (coincidence counting, moderation, die-away time); detector systems – some important details (deadtime, corrections); introduction to multiplicity counting; multiplicity electronics and example distributions; singles, doubles, and triples from measured multiplicity distributions; and the point model: multiplicity mathematics.

  17. Effects of AT1 Receptor Blockade on Plasma Thromboxane A2 (TXA2 Level and Skin Microcirculation in Young Healthy Women on Low Salt Diet

    Directory of Open Access Journals (Sweden)

    Ana Cavka

    2013-10-01

    Full Text Available Objective: To determine the effect of AT1 receptor antagonism on skin microcirculation and plasma level of thromboxane A2 (TXA2. Methods: Healthy women (n=20 maintained 7 days low salt (LS diet (intake 2 metabolite thromboxane B2 (TXB2 and plasma renin activity (PRA aldosterone concentration, electrolytes (Na+, K+, as well as blood pressure and heart rate were determined before and after study protocols. Results: PRA and aldosterone increased significantly after 7 days of both LS diet and LS diet+losartan. LS diet or LS diet+losartan administrations had no significant effect on post-occlusion hyperemia While there was no change in TXB2 after LS diet TXB2 significantly increased after one week of LS+losartan compared to control levels (cTXB2 pg/mL control 101±80 vs. LS diet+losartan 190±116, pConclusion: These data suggest that inhibition of AT1 receptors could lead to activation of AT2 receptors, which maintain hyperemia, despite the increased level of vasoconstrictor TXA2. These findings also suggest an important role of crosstalk between renin-angiotensin system (RAS and arachidonic acid metabolites in the regulation of microcirculation under physiological conditions.

  18. Hypersensitivity to thromboxane receptor mediated cerebral vasomotion and CBF oscillations during acute NO-deficiency in rats.

    Directory of Open Access Journals (Sweden)

    Béla Horváth

    Full Text Available BACKGROUND: Low frequency (4-12 cpm spontaneous fluctuations of the cerebrovascular tone (vasomotion and oscillations of the cerebral blood flow (CBF have been reported in diseases associated with endothelial dysfunction. Since endothelium-derived nitric oxide (NO suppresses constitutively the release and vascular effects of thromboxane A(2 (TXA(2, NO-deficiency is often associated with activation of thromboxane receptors (TP. In the present study we hypothesized that in the absence of NO, overactivation of the TP-receptor mediated cerebrovascular signaling pathway contributes to the development of vasomotion and CBF oscillations. METHODOLOGY/PRINCIPAL FINDINGS: Effects of pharmacological modulation of TP-receptor activation and its downstream signaling pathway have been investigated on CBF oscillations (measured by laser-Doppler flowmetry in anesthetized rats and vasomotion (measured by isometric tension recording in isolated rat middle cerebral arteries, MCAs both under physiological conditions and after acute inhibition of NO synthesis. Administration of the TP-receptor agonist U-46619 (1 µg/kg i.v. to control animals failed to induce any changes of the systemic or cerebral circulatory parameters. Inhibition of the NO synthesis by nitro-L-arginine methyl ester (L-NAME, 100 mg/kg i.v. resulted in increased mean arterial blood pressure and a decreased CBF accompanied by appearance of CBF-oscillations with a dominant frequency of 148±2 mHz. U-46619 significantly augmented the CBF-oscillations induced by L-NAME while inhibition of endogenous TXA(2 synthesis by ozagrel (10 mg/kg i.v. attenuated it. In isolated MCAs U-46619 in a concentration of 100 nM, which induced weak and stable contraction under physiological conditions, evoked sustained vasomotion in the absence of NO, which effect could be completely reversed by inhibition of Rho-kinase by 10 µM Y-27632. CONCLUSION/SIGNIFICANCE: These results suggest that hypersensitivity of the TP

  19. Reticulocyte count

    Science.gov (United States)

    ... radiation therapy, or infection) Cirrhosis of the liver Anemia caused by low iron levels, or low levels of vitamin B12 or folate Chronic kidney disease Reticulocyte count may be higher during pregnancy.

  20. Differential white blood cell count and type 2 diabetes: systematic review and meta-analysis of cross-sectional and prospective studies.

    Directory of Open Access Journals (Sweden)

    Effrossyni Gkrania-Klotsas

    Full Text Available OBJECTIVE: Biological evidence suggests that inflammation might induce type 2 diabetes (T2D, and epidemiological studies have shown an association between higher white blood cell count (WBC and T2D. However, the association has not been systematically investigated. RESEARCH DESIGN AND METHODS: Studies were identified through computer-based and manual searches. Previously unreported studies were sought through correspondence. 20 studies were identified (8,647 T2D cases and 85,040 non-cases. Estimates of the association of WBC with T2D were combined using random effects meta-analysis; sources of heterogeneity as well as presence of publication bias were explored. RESULTS: The combined relative risk (RR comparing the top to bottom tertile of the WBC count was 1.61 (95% CI: 1.45; 1.79, p = 1.5*10(-18. Substantial heterogeneity was present (I(2 = 83%. For granulocytes the RR was 1.38 (95% CI: 1.17; 1.64, p = 1.5*10(-4, for lymphocytes 1.26 (95% CI: 1.02; 1.56, p = 0.029, and for monocytes 0.93 (95% CI: 0.68; 1.28, p = 0.67 comparing top to bottom tertile. In cross-sectional studies, RR was 1.74 (95% CI: 1.49; 2.02, p = 7.7*10(-13, while in cohort studies it was 1.48 (95% CI: 1.22; 1.79, p = 7.7*10(-5. We assessed the impact of confounding in EPIC-Norfolk study and found that the age and sex adjusted HR of 2.19 (95% CI: 1.74; 2.75 was attenuated to 1.82 (95% CI: 1.45; 2.29 after further accounting for smoking, T2D family history, physical activity, education, BMI and waist circumference. CONCLUSIONS: A raised WBC is associated with higher risk of T2D. The presence of publication bias and failure to control for all potential confounders in all studies means the observed association is likely an overestimate.

  1. Evaluation of platelet thromboxane radioimmunoassay method to measure platelet life-span: Comparison with /sup 111/indium-platelet method

    Energy Technology Data Exchange (ETDEWEB)

    Vallabhajosula, S.; Machac, J.; Badimon, L.; Lipszyc, H.; Goldsmith, S.J.; Fuster, V.

    1985-05-01

    The platelet activation during radiolabeling in vitro with Cr-51 and In-111 may affect the platelet life-span (PLS) in vivo. A new RIA method to measure PLS is being evaluated. Aspirin inhibits platelet thromboxane (TxA/sub 2/) by acetylating cyclooxygenase. The time required for the TxA/sub 2/ levels to return towards control values depends on the rate of new platelets entering circulation and is a measure of PLS. A single dose of aspirin (150mg) was given to 5 normal human subjects. Blood samples were collected for 2 days before aspirin and daily for 10 days. TxA/sub 2/ production in response to endogenous thrombin was studied by allowing 1 ml blood sample to clot at 37/sup 0/C for 90 min. Serum TxB/sub 2/ (stable breakdown product of Tx-A/sub 2/) levels determined by RIA technique. The plot of TxB/sub 2/ levels (% control) against time showed a gradual increase. The PLS calculated by linear regression analysis assuming a 2-day lag period before cyclooxygenase recovery is 9.7 +- 2.37. In the same 5 subjects, platelets from a 50ml blood sample were labeled with /sup 111/In-tropolone in 2 ml autologous plasma. Starting at 1 hr after injection of labeled platelets, 10 blood samples were obtained over a 8 day period. The PLS calculated based on a linear regression analysis is 10.2 +. 1.4. The PLS measured from the rate of platelet disappearance from circulation and the rate of platelet regeneration into circulation are quite comparable in normal subjects. TxA/sub 2/ regeneration RIA may provide a method to measure PLS without administering radioactivity to patient.

  2. Evaluation of the automated hematology analyzer Sysmex XT-2000iV™ compared to the ADVIA® 2120 for its use in dogs, cats, and horses: Part I--precision, linearity, and accuracy of complete blood cell count.

    Science.gov (United States)

    Bauer, Natali; Nakagawa, Julia; Dunker, Cathrin; Failing, Klaus; Moritz, Andreas

    2011-11-01

    The automated laser-based hematology analyzer Sysmex XT-2000iV™ providing a complete blood cell count (CBC) and 5-part differential has been introduced in large veterinary laboratories. The aim of the current study was to determine precision, linearity, and accuracy of the Sysmex analyzer. Reference method for the accuracy study was the laser-based hematology analyzer ADVIA® 2120. For evaluation of accuracy, consecutive fresh blood samples from healthy and diseased cats (n = 216), dogs (n = 314), and horses (n = 174) were included. A low intra-assay coefficient of variation (CV) of approximately 1% was seen for the CBC except platelet count (PLT). An intra-assay CV ranging between 2% and 5.5% was evident for the differential count except for feline and equine monocytes (7.7%) and horse eosinophils (15.7%). Linearity was excellent for white blood cell count (WBC), hematocrit value, red blood cell count (RBC), and PLT. For all evaluated species, agreement was excellent for WBC and RBC, with Spearman rank correlation coefficients (r(s)) ranging from >0.99 to 0.98. Hematocrit value correlated excellently in cats and dogs, whereas for horses, a good correlation was evident. A good correlation between both analyzers was seen in feline and equine PLT (r(s) = 0.89 and 0.92, respectively), whereas correlation was excellent for dogs (r(s) = 0.93). Biases were close to 0 except for mean corpuscular hemoglobin concentration (4.11 to -7.25 mmol/l) and canine PLT (57 × 10(9)/l). Overall, the performance of the Sysmex analyzer was excellent and compared favorably with the ADVIA analyzer.

  3. Correlation between differential blood count and T-lymphocyte subset count in the early period after liver transplantation%肝移植术后早期白细胞分类计数与T淋巴细胞亚群计数的相关性研究

    Institute of Scientific and Technical Information of China (English)

    邹游; 张磊; 李瑞东; 郭闻渊

    2012-01-01

    目的 探讨肝移植术后早期白细胞分类计数与T淋巴细胞亚群计数的相关性.方法 收集84例行经典原位肝移植术受者术后第7天在不应用糖皮质激素情况下的血样本,分析T淋巴细胞亚群计数与白细胞计数、中性粒细胞计数、淋巴细胞计数及单核细胞计数的相关性.结果 Pearson分析显示,CD4+和CD8+T淋巴细胞计数与白细胞计数、中性粒细胞计数及单核细胞计数间均不相关(P值均>0.05),与淋巴细胞计数间均呈正相关(R值分别为0.778、0.651,P值均<0.001).Spearman分析显示,CD4+和CD8+T淋巴细胞计数与白细胞计数、中性粒细胞计数及单核细胞计数间均不相关(P值均>0.05),与淋巴细胞计数间均呈正相关(R值分别为0.748、0.702,P值均<0.001).非感染组的淋巴细胞计数、CD4+T淋巴细胞计数、CD8+T淋巴细胞计数均显著高于感染组(P值均<0.05).结论 肝移植术后早期淋巴细胞计数与CD4+T淋巴细胞计数具有较好的正相关性,可间接反应CD4+T淋巴细胞计数水平.%Objective To explore the correlation between differential blood count and T-lymphocyte subset count so as to provide a method to quickly identify cellular immune function in the early period after liver transplantation. Methods Blood samples were taken from 84 recipients (without application of glucocorticoid) on day 7 after orthotopic liver transplantation (OLT). The correlation between T-lymphocyte subset count and differential blood count including leucocyte count, granulocyte count and monocyte count was analyzed. Results Pearson analysis showed that the counts of CD4+ and CD8+ T lymphocytes were not correlated with the count of leucocyte, granulocyte and monocyte (all P>0.05), but were positively related to lymphocyte count (R =0.778, 0.651; both P0.05), and were positively related to lymphocyte count (R =0.748, 0.702; both P<0.001). The counts of lymphocyte, CD4+ T lymphocyte and CD8+ T lymphocyte in the

  4. Examination of thromboxane synthase as a prognostic factor and therapeutic target in non-small cell lung cancer.

    LENUS (Irish Health Repository)

    Cathcart, Mary-Clare

    2011-03-01

    Thromboxane synthase (TXS) metabolises prostaglandin H2 into thromboxanes, which are biologically active on cancer cells. TXS over-expression has been reported in a range of cancers, and associated with a poor prognosis. TXS inhibition induces cell death in-vitro, providing a rationale for therapeutic intervention. We aimed to determine the expression profile of TXS in NSCLC and if it is prognostic and\\/or a survival factor in the disease.

  5. Performance evaluation of XN-1000 Sysmex blood cell count of low value of white blood cell%Sysmex XN-1000血细胞分析仪计数低值白细胞性能的评估

    Institute of Scientific and Technical Information of China (English)

    蒋清清; 马萍; 丁爽

    2016-01-01

    目的:探讨 Sysmex XN‐1000(后简称 XN‐1000)全自动血细胞分析仪检测白细胞计数(WBC)及分类的准确性。方法收集该院2015年1~4月门诊及住院患者及健康体检者共206例,根据人工镜检检测 WBC 结果分为高值组(WBC >10.0×109/L),健康组(4.0×109/L < WBC ≤10.0×109/L)和低值组(1.5×109/L < WBC ≤4.0×109/L)。再分别用 XN‐1000与Sysmex XE‐5000(后简称 XE‐5000)进行 WBC 并分类。采用统计学软件对3种方法的结果进行对比分析,并进行相关性分析。结果3种方法检测 WBC 结果比较,高值组和健康组差异无统计学意义(P>0.05),结果具有正相关性。低值组中,XN‐1000检测结果准确性与 XE‐5000差异无统计学意义(P>0.05),但是白细胞分类结果显著高于 XE‐5000(P<0.05);对于异常细胞,XN‐1000和 XE‐5000不能进行有效分类,仍需人工镜检进行分类。结论 XN‐1000全自动血细胞分析仪在 WBC 与 XE‐5000及人工镜检比较无差异统计学意义,在测定白细胞分类上 XN‐1000较 XE‐5000更准确(XN‐1000合格率91%,XE‐5000合格率63%),但仍不能完全替代人工镜检。对于异常细胞,XN‐1000不能有效分类,需与人工镜检结合。%Objective To investigate the accuracy of XN‐1000 automatic blood cell analyzer in detecting of white blood cell (WBC) count and classification .Methods A total of 206 cases including outpatients ,inpatients and health people were collected in the hospital from January to April 2015 .According to manual microscopy detection of white blood cell count results ,the cases were divided into three groups :high value of white blood cell(WBC was more than 10 .0 × 109 /L) group ,normal value of white blood cells group(WBC was more than 4 .0 × 109 /L but less than 10 .0 × 109 /L) and low value of white blood cell(WBC was more than 1 .5 × 109 /L

  6. Utility of the tourniquet test and the white blood cell count to differentiate dengue among acute febrile illnesses in the emergency room.

    Directory of Open Access Journals (Sweden)

    Christopher J Gregory

    2011-12-01

    Full Text Available Dengue often presents with non-specific clinical signs, and given the current paucity of accurate, rapid diagnostic laboratory tests, identifying easily obtainable bedside markers of dengue remains a priority. Previous studies in febrile Asian children have suggested that the combination of a positive tourniquet test (TT and leucopenia can distinguish dengue from other febrile illnesses, but little data exists on the usefulness of these tests in adults or in the Americas. We evaluated the diagnostic accuracy of the TT and leucopenia (white blood cell count <5000/mm(3 in identifying dengue as part of an acute febrile illness (AFI surveillance study conducted in the Emergency Department of Saint Luke's Hospital in Ponce, Puerto Rico. From September to December 2009, 284 patients presenting to the ED with fever for 2-7 days and no identified source were enrolled. Participants were tested for influenza, dengue, leptospirosis and enteroviruses. Thirty-three (12% patients were confirmed as having dengue; 2 had dengue co-infection with influenza and leptospirosis, respectively. An infectious etiology was determined for 141 others (136 influenza, 3 enterovirus, 2 urinary tract infections, and 110 patients had no infectious etiology identified. Fifty-two percent of laboratory-positive dengue cases had a positive TT versus 18% of patients without dengue (P<0.001, 87% of dengue cases compared to 28% of non-dengue cases had leucopenia (P<0.001. The presence of either a positive TT or leucopenia correctly identified 94% of dengue patients. The specificity and positive predictive values of these tests was significantly higher in the subset of patients without pandemic influenza A H1N1, suggesting improved discriminatory performance of these tests in the absence of concurrent dengue and influenza outbreaks. However, even during simultaneous AFI outbreaks, the absence of leucopenia combined with a negative tourniquet test may be useful to rule out dengue.

  7. Acute effects of resistance exercise and intermittent intense aerobic exercise on blood cell count and oxidative stress in trained middle-aged women

    Energy Technology Data Exchange (ETDEWEB)

    Cardoso, A.M. [Departamento de Química, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Santa Maria, RS (Brazil); Bagatini, M.D. [Curso de Enfermagem, Campus Chapecó, Universidade Federal da Fronteira Sul, Chapecó, SC (Brazil); Roth, M.A. [Departamento de Desportos Individuais, Centro de Educação Física e Desportos, Universidade Federal de Santa Maria, Santa Maria, RS (Brazil); Martins, C.C.; Rezer, J.F.P. [Departamento de Química, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Santa Maria, RS (Brazil); Mello, F.F. [Departamento de Desportos Individuais, Centro de Educação Física e Desportos, Universidade Federal de Santa Maria, Santa Maria, RS (Brazil); Lopes, L.F.D. [Departamento de Administração, Centro de Ciências Sociais e Humanas, Universidade Federal de Santa Maria, Santa Maria, RS (Brazil); Morsch, V.M.; Schetinger, M.R.C. [Departamento de Química, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Santa Maria, RS (Brazil)

    2012-10-26

    The aim of this study was to compare the effect of an intermittent intense aerobic exercise session and a resistance exercise session on blood cell counts and oxidative stress parameters in middle-aged women. Thirty-four women were selected and divided into three groups: RE group (performing 60 min of resistance exercises, N = 12), spinning group (performing 60 min of spinning, N = 12), and control group (not exercising regularly, N = 10). In both exercise groups, lymphocytes and monocytes decreased after 1-h recuperation (post-exercise) compared to immediately after exercise (P < 0.05). Immediately after exercise, in both exercised groups, a significant increase in TBARS (from 16.5 ± 2 to 25 ± 2 for the spinning group and from 18.6 ± 1 to 28.2 ± 3 nmol MDA/mL serum for the RE group) and protein carbonyl (from 1.0 ± 0.3 to 1.6 ± 0.2 for the spinning group and from 0.9 ± 0.2 to 1.5 ± 0.2 nmol/mg protein for the RE group) was observed (P < 0.05). A decrease in antioxidant activities (non-protein sulfhydryl, superoxide dismutase, catalase) was also demonstrated with a negative correlation between damage markers and antioxidant body defenses (P < 0.05). These results indicate that an acute bout of intermittent or anaerobic exercise induces immune suppression and increases the production of reactive oxygen species, causing oxidative stress in middle-aged and trained women. Furthermore, we demonstrated that trained women show improved antioxidant capacity and lower oxidative damage than sedentary ones, demonstrating the benefits of chronic regular physical activity.

  8. Monoclonal B lymphocytes with the characteristics of "indolent" chronic lymphocytic leukemia are present in 3.5% of adults with normal blood counts.

    Science.gov (United States)

    Rawstron, Andy C; Green, Michael J; Kuzmicki, Anita; Kennedy, Ben; Fenton, James A L; Evans, Paul A S; O'Connor, Sheila J M; Richards, Stephen J; Morgan, Gareth J; Jack, Andrew S; Hillmen, Peter

    2002-07-15

    Molecular and cellular markers associated with malignant disease are frequently identified in healthy individuals. The relationship between these markers and clinical disease is not clear, except where a neoplastic cell population can be identified as in myeloma/monoclonal gammopathies of undetermined significance (MGUS). We have used the distinctive phenotype of chronic lymphocytic leukemia (CLL) cells to determine whether low levels of these cells can be identified in individuals with normal complete blood counts. CLL cells were identified by 4-color flow cytometric analysis of CD19/CD5/CD79b/CD20 expression in 910 outpatients over 40 years old. These outpatients were age- and sex-matched to the general population with normal hematologic parameters and no evident history of malignant disease. CLL phenotype cells were detectable in 3.5% of individuals at low level (median, 0.013; range, 0.002- 1.458 x 10(9) cells/L), and represented a minority of B lymphocytes (median, 11%; range, 3%-95%). Monoclonality was demonstrated by immunoglobulin light-chain restriction in all cases with CLL phenotype cells present and confirmed in a subset of cases by consensus-primer IgH-polymerase chain reaction. As in clinical disease, CLL phenotype cells were detected with a higher frequency in men (male-to-female ratio, 1.9:1) and elderly individuals (2.1% of 40- to 59-year-olds versus 5.0% of 60- to 89-year-olds, P =.01). The neoplastic cells were identical to good-prognosis CLL, being CD5+23+20(wk)79b(wk)11a(-)22(wk)sIg(wk)CD38-, and where assessed had a high degree (4.8%-6.6%) of IgH somatic hypermutation. The monoclonal CLL phenotype cells present in otherwise healthy individuals may represent a very early stage of indolent CLL and should be useful in elucidating the mechanisms of leukemogenesis.

  9. Complete blood counts, liver function tests, and chest x-rays as routine screening in early-stage breast cancer: value added or just cost?

    Science.gov (United States)

    Louie, Raphael J; Tonneson, Jennifer E; Gowarty, Minda; Goodney, Philip P; Barth, Richard J; Rosenkranz, Kari M

    2015-11-01

    Current National Comprehensive Cancer Network guidelines for breast cancer staging include pre-treatment complete blood count (CBC) and liver function tests (LFT) to screen for occult metastatic disease. To date, the relevance of these tests in detecting metastatic disease in asymptomatic women with early-stage breast cancer (Stage I/II) has not been demonstrated. Although chest x-rays are no longer recommended in the NCCN guidelines, many centers continue to include this imaging as part of their screening process. We aim to determine the clinical and financial impact of these labs and x-rays in the evaluation of early-stage breast cancer patients. A single institution IRB-approved retrospective chart review was conducted of patients with biopsy-proven invasive breast cancer treated from January 1, 2005–December 31, 2009. We collected patient demographics, clinical and pathologic staging, chest x-ray, CBC, and LFT results at the time of referral. Patients were stratified according to radiographic stage at the time of diagnosis. We obtained Medicare reimbursement fees for cost analysis. From 2005 to 2009, 1609 patients with biopsy-proven invasive breast cancer were treated at our institution. Of the 1082 patients with radiographic stage I/II disease, 27.3 % of patients had abnormal CBCs. No additional testing was performed to evaluate these abnormalities. In the early-stage population, 24.7 % of patients had elevated LFTs, resulting in 84 additional imaging studies. No metastatic disease was detected. The cost of CBC, LFTs and chest x-rays was $110.20 per patient, totaling $106,410.99. Additional tests prompted by abnormal results cost $58,143.30 over the five-year period. We found that pre-treatment CBCs, LFTs, and chest x-rays did not improve detection of occult metastatic disease but resulted in additional financial costs. Avoiding routine ordering of these tests would save the US healthcare system $25.7 million annually.

  10. Analysis of the routine blood counts and blood biochemical parameters and hemorheology in the rats with congenital cataract%先天性白内障大鼠血液生化及血液流变学分析

    Institute of Scientific and Technical Information of China (English)

    田小芸; 尤金炜; 董敏; 许龙祥; 赵志刚; 陈莉; 恽时锋

    2014-01-01

    Objective To measure the range of routine blood counts and blood biochemical parameters ,to analyze hemorheology of the rats with congenital cataract .Methods Blood samples were taken from 90 rats with congenital cataract weight about 185 ~211 g.Routine blood analysis was performed and blood biochemical and hemorheology paramenters were determind using an automatic blood biochemical and hemorheology analyzer .Results There were no significant difference ( P >0.05 ) between the cataract rats and the normal rats in Blood test results; but there were significant difference between the microphthalmos cataract rats and the normal same -sex rats ( P <0.01 or P <0.05 ) . The biochemical results is the cataract rats and the normal rats were different significantly in ALB group ( P <0.01 or P<0.05), and the female microphthalmos cataract rats compared with the control rats had significant difference in Ure (P<0.01) , the female cataract rats ompared with the normal rats were very significant difference in Cr group ( P <0.05 or P <0.01).The erythrocyte counts of the male cataract rats and male microphthalmos cataract rats were significantly lower than that in the female ones, respectively(P <0.05, P <0.01).The platelet counts of the male cataract rats and the male microphthalmos cataract rats were significantly higher than that in the female ones , respectively(P <0.01), and the creatinine of the male cataract rats and the male microphthalmos cataract rats were significantly lower then that in the female ones, respectively(P <0.01).There were no significant difference in every group on hemorheology .Conclusions There were significant differences in some blood indexes between the congenital cataract rats and the normal rats .These data may become useful reference for biomedical researcher in this field .%目的:测定先天性白内障大鼠血液常规、生化正常值及血液流变学变化。方法采用XS-800i血常规分析仪和OLYMPUS AV2700

  11. Role of plasma C-reactive protein and white blood cell count in predicting in-hospital clinical events of acute type A aortic dissection

    Institute of Scientific and Technical Information of China (English)

    WEN Dan; WU Hai-ying; JIANG Xiong-jing; ZHANG Hui-min; ZHOU Xian-liang; LI Jian-jun; HUI Ru-tai

    2011-01-01

    Background A few recent studies have reported that inflammation is associated with the prognosis of acute aortic dissection (AD).There is,however,no systemic investigation regarding the role of plasma C-reactive protein (CRP) and white blood cell (WBC) levels in predicting in-hospital clinical events of acute type AAD.Methods The levels of high-sensitivity CRP and WBC counts were systemically determined after admission in 36 patients with acute type A AD.The variations of plasma CRP and WBC levels in different time windows (admission,1,2,3,4,6,8 days) in patients with acute type A AD were analyzed between patients with events and without events.Results During hospitalization,five patients died,and increased levels of CRP and WBC were found in patients died with acute type A AD compared with patients survived (P <0.01,respectively).Medical treatment may significantly decrease inflammatory response in survived patients with acute type A AD.Additionally,patients with complication of pleural effusion showed higher CRP and WBC levels (P=0.046,P=0.018,respectively).Lower WBC levels were found in survived patients treated medically (P=0.001).Moreover,mean CRP and WBC levels had positive correlations with aortic diameter (r=0.364,P=0.000;r=0.333,P=0.000,respectively) and age (r=0.270,P=0.000,respectively),while negative correlations with the time from onset of symptoms to hospital admission (r=-0.229,P=0.000,r=-0.200,P=0.002,respectively).Univariate analysis showed that age ≥65 years,CRP ≥12.05 mg/L,WBC ≥12.16×109/L,aortic diameter ≥48mm,pleural effusion and diastolic blood pressure ≥105 mmHg were associated with hospital mortality.While CRP ≥12.05 mg/L,WBC ≥12.16×109/L,aortic diameter ≥48 mm were strongly associated with hospital mortality in multiple Logistic regression analysis.Conclusions The results suggested that CRP and WBC were preferred markers for predicting the clinical events in patients with acute type A AD,especially death during

  12. The accuracy of platelet counting in thrombocytopenic blood samples distributed by the UK National External Quality Assessment Scheme for General Haematology.

    Science.gov (United States)

    De la Salle, Barbara J; McTaggart, Paul N; Briggs, Carol; Harrison, Paul; Doré, Caroline J; Longair, Ian; Machin, Samuel J; Hyde, Keith

    2012-01-01

    A knowledge of the limitations of automated platelet counting is essential for the effective care of thrombocytopenic patients and management of platelet stocks for transfusion. For this study, 29 external quality assessment specimen pools with platelet counts between 5 and 64 × 10(9)/L were distributed to more than 1,100 users of 23 different hematology analyzer models. The same specimen pools were analyzed by the international reference method (IRM) for platelet counting at 3 reference centers. The IRM values were on average lower than the all-methods median values returned by the automated analyzers. The majority (~67%) of the automated analyzer results overestimated the platelet count compared with the IRM, with significant differences in 16.5% of cases. Performance differed between analyzer models. The observed differences may depend in part on the nature of the survey material and analyzer technology, but the findings have implications for the interpretation of platelet counts at levels of clinical decision making.

  13. Anti-thromboxane B2 antibodies protect against acetaminophen-induced liver injury in mice

    Directory of Open Access Journals (Sweden)

    Ivan Ćavar

    2011-12-01

    Full Text Available Prostanoids are lipid compounds that mediate a variety of physiological and pathological functions in almost all body tissues and organs. Thromboxane (TX A2 is a powerful inducer of platelet aggregation and vasoconstriction and it has ulcerogenic activity in the gastrointestinal tract. Overdose or chronic use of a high dose of acetaminophen (N-acetyl-paminophenol, APAP is a major cause of acute liver failure in the Western world. We investigated whether TXA2 plays a role in host response to toxic effect of APAP. CBA/H Zg mice of both sexes were intoxicated with a single lethal or high sublethal dose of APAP, which was administered to animals by oral gavage. The toxicity of APAP was determined by observing the survival of mice during 48 h, by measuring concentration of alanine-aminotransferase (ALT in plasma 20-22 h after APAP administration and by liver histology. The results have shown that anti-thromboxane (TX B2 antibodies (anti-TXB2 and a selective inhibitor of thromboxane (TX synthase, benzylimidazole (BZI, were significantly hepatoprotective, while a selective thromboxane receptor (TPR antagonist, daltroban, was slightly protective in this model of acute liver injury. A stabile metabolite of TXA2, TXB2, and a stabile agonist of TPR, U-46619, had no influence on APAP-induced liver damage. Our findings suggest that TXA2 has a pathogenic role in acute liver toxicity induced with APAP, which was highly abrogated by administration of anti-TXB2. According to our results, this protection is mediated, at least in part, through decreased production of TXB2 by liver fragments ex vivo.

  14. 不同医院全血细胞计数比对实验结果分析%Comparison test of blood cell counts between different hospitals

    Institute of Scientific and Technical Information of China (English)

    雷娜; 张彦平; 井敏敏; 胡恩亮; 程翔; 张利军; 张磊

    2016-01-01

    目的:分析3家医院不同分析仪全血细胞计数比对实验结果。方法以西安交通大学第二附属医院(简称交大二院)采用的日本希森美康公司XE‐2100型全自动血细胞分析仪(简称XE‐2100分析仪)作为参比仪器,以西京医院采用的日本希森美康公司XN‐3000分析仪(检测XN‐3000分析仪)及唐都医院采用的XE‐2100作为实验仪器,按照美国临床和实验室标准化协会(CLSI)发布的EP9‐A2文件的要求,对白细胞(WBC)、红细胞(RBC)、血红蛋白(Hb)、血小板(PLT)、红细胞比容(HCT)检测结果进行比对分析,计算检测结果相关系数(r),以美国临床实验室修正法规(CLIA′88)规定的室间质量评价总允许误差范围为标准,判断检测结果偏差是否可接受。结果交大二院XE‐2100分析仪和西京医院XN‐3000分析仪WBC、RBC、Hb、HCT检测结果的 r值分别为0.998、0.998、0.984、0.981,相关性良好,PLT检测结果的 r值为0.953,相关性不佳;WBC、RBC、Hb、HCT检测结果比较差异无统计学意义(P>0.05),但PLT检测结果比较差异有统计学意义(P<0.05)。交大二院XE‐2100分析仪和唐都医院XE‐2100分析仪WBC、RBC、PLT、Hb、HCT检测结果的 r值分别为0.999、0.999、0.998、0.999、0.998,相关性良好;各参数检测结果比较差异无统计学意义(P>0.05)。结论不同医院全血细胞计数部分参数检测结果相关性良好,检测结果可以互认。%Objective To analyze the results of comparison test of blood cell counts between three hospitals .Methods Accord‐ing to the EP9‐A2 file of Clinical and Laboratory Standards Institute(CLSI) ,comparison tests of white blood cells(WBC) ,red blood cells(RBC) ,hemoglobin(Hb) ,platelet(PLT) and hematocrit(HCT)were performed ,taking Sysmex XE‐2100 analyzer ,used in the Second Affiliated Hospital of Xi

  15. Terutroban, a thromboxane/prostaglandin endoperoxide receptor antagonist, prevents hypertensive vascular hypertrophy and fibrosis.

    Science.gov (United States)

    Gelosa, Paolo; Sevin, Gulnur; Pignieri, Alice; Budelli, Silvia; Castiglioni, Laura; Blanc-Guillemaud, Vanessa; Lerond, Laurence; Tremoli, Elena; Sironi, Luigi

    2011-03-01

    Thromboxane A(2) and other eicosanoids such as isoprostanes contribute to vascular proliferation and atherosclerosis by binding to the thromboxane/prostaglandin endoperoxide receptors. The effects of terutroban, a thromboxane/prostaglandin endoperoxide receptor antagonist, on aorta remodeling were evaluated in spontaneously hypertensive stroke-prone rats (SHRSPs), a model of severe hypertension, endothelial dysfunction, vascular inflammation, and cerebrovascular diseases. Male SHRSPs were allocated to three groups receiving a standard diet (n = 5) or a high-sodium permissive diet plus vehicle (n = 6) or plus terutroban (30 mg · kg(-1) · day(-1); n = 6). After 6 wk of dietary treatment, all of the animals were injected with bromodeoxyuridine and simultaneously euthanized for aorta collection. The aortic media thickness-to-lumen ratio significantly (P hyperplasia and has beneficial effects on fibrotic processes by affecting TGF-β and heat shock protein-47 expression in SHRSPs. These findings provide mechanistic data supporting the beneficial effects of terutroban in preventing or retarding atherogenesis.

  16. AGN 191976: a novel thromboxane A2-mimetic with ocular hypotensive properties.

    Science.gov (United States)

    Krauss, A H; Woodward, D F; Chen, J; Gibson, L L; Lai, R K; Protzman, C E; Shan, T; Williams, L S; Gac, T S; Burk, R M

    1995-01-01

    The possible subdivision of thromboxane A2-sensitive (TP) receptors is currently a controversial subject. We report herein on a novel thromboxane A2 mimetic, AGN 191976, which has almost identical pharmacological activity to the well-characterized prostaglandin H2/thromboxane A2 (PGH2/TxA2) mimetic U-46619, but its effects on intraocular pressure are quite distinct from U-46619. Prostanoid receptor activity was determined in vitro using different smooth muscle assays and platelets. Intraocular pressure was measured tonometrically in ocular normotensive Beagle dogs and Cynomolgus monkeys. Conjunctival microvascular permeability was determined in guinea pigs. Despite closely resembling U-46619 as a potent and selective TP receptor agonist, AGN 191976 was a potent ocular hypotensive in dogs and monkeys whereas U-46619 did not lower IOP in either species. The ocular hypotensive effect of AGN 191976 in dogs was attenuated by pretreatment with the TP receptor antagonist SQ 29548. Thus, the ocular hypotensive effects of AGN 191976 are consistent with TP receptor stimulation. Both TxA2-mimetics caused plasma leakage in the guinea pig conjunctiva. The disparate activities of U-46619 and AGN 191976 in our studies suggest the existence of heterogeneous populations of TP-receptors in the eye.

  17. 探讨全血白细胞计数危急值与临床的相互关系%Investigation of the relationship between whole blood leukocyte counts and clinical critical values

    Institute of Scientific and Technical Information of China (English)

    李芳林

    2015-01-01

    AIM:To investigate the relationship between critical value blood leukocyte counts and clinical practice.METHODS:From June 2011 to May 2013 in our hospital all critical values, detected by medical laboratory,were selected and analyzed retro-spectively including the clinical data of whole blood cell count in patients with critical values,and patients'characteristics and dis-tribution.RESULTS:A total of 1 462 reported values matched the critical value critical condition,82 critical values of whole blood leukocyte count,81 540 count tests in total,and whole blood leukocyte count was reported critical values of 0.10%,ac-counting for 5.61% of the total critical value;whole blood leuko-cyte count critical values were mainly in leukemia,acute infec-tions,and cancer;high values of critical values distributed in 59 cases of acute infection,leukemia,acute bleeding,burns,canc-er,and kidney failure patients,with the distribution interval being (30.1 ~310.0)×109 /L;the low critical value distributed in 23 cases of leukemia and cancer patients,with the distribution range being (0.04 ~0.50)×109 /L.CONCLUSION:Quoted rate of white blood cell count can be affected by its critical value range, therefore the whole range of critical white blood cell count should be reasonably set,and its distribution characteristics should be analyzed and timely fedback to the clinic,in order to offer better clinical services.%目的:探讨全血白细胞计数危急值与临床的相互关系.方法:选取2011-06/2013-05我院医学检验科检测到的所有危急值,对全血白细胞计数危急值患者的临床资料进行回顾性分析,包括患者的特点和分布情况.结果:共报出符合危急值报告条件的危急值1462个,全血白细胞计数危急值82个,共进行全血白细胞计数测试81540个,全血白细胞计数危急值报出率为0.10%,占总危急值的5.61%;全血白细胞计数危急值主要分布在白血病、急性感染、

  18. Counting Populations

    Science.gov (United States)

    Damonte, Kathleen

    2004-01-01

    Scientists use sampling to get an estimate of things they cannot easily count. A population is made up of all the organisms of one species living together in one place at the same time. All of the people living together in one town are considered a population. All of the grasshoppers living in a field are a population. Scientists keep track of the…

  19. Counting Penguins.

    Science.gov (United States)

    Perry, Mike; Kader, Gary

    1998-01-01

    Presents an activity on the simplification of penguin counting by employing the basic ideas and principles of sampling to teach students to understand and recognize its role in statistical claims. Emphasizes estimation, data analysis and interpretation, and central limit theorem. Includes a list of items for classroom discussion. (ASK)

  20. ANALYSIS OF CHANGES BETWEEN FETAL NUCLEATED RED BLOOD CELL COUNT IN MATERNAL BLOOD AND PREGNANCY COMPLICATIONS%孕妇外周血胎儿有核红细胞与妊娠并发症变化分析

    Institute of Scientific and Technical Information of China (English)

    王志宏; 邵雪斋; 李陈莉

    2013-01-01

    Objective To investigate the changes of fetal nucleated red blood cells count (FNRBC) in peripheral blood of pregnant women with different pregnancy complications, such as hypertensive disorders in pregnancy, gestational diabetes mellitus (GDM) and pregnancy with thrombocytopenia. Methods Thirty clinically diagnosed hypertensive disorders in pregnancy patients (12 cases mild preeclampsia, 12 cases severe preeclampsia,6 cases eclampsia),23 cases GDM patients (13 cases blood sugar control ideal and without hypoglycemia or ketosis,10 cases whose blood sugar control was not ideal and ever occurred hypoglycemia or ketosis) and 18 cases pregnancy with thrombocytopenia patients were analyzed in this study. Twenty cases normal pregnant women were taken as control at the same time. The peripheral blood of women in each group was obtained to count FNBRC and perform Wright-Giemsa stain after single density gradient centrifugation. Results There were significant differences in FNRBC number in peripheral blood of mild preeclampsia patients, severe preeclampsia patients and eclampsia patients compared with normal pregnant women in control group (P0. 05). Conclusion The FNRBC number of preeclampsia patients,eclampsia patients and GDM patients increase; moreover, the increase of FNRBC number in maternal peripheral blood is more obvious when illness is aggravated. The results suggest that increasing of FNRBC number can indicate aggravation of pregnancy complications and threat fetal life. Doctors should take effective interventions actively, and terminate pregnancy if necessary.%目的 探讨妊娠期高血压疾病、妊娠期糖尿病、妊娠合并血小板减少症时,母血中胎儿有核红细胞(fetal nucleated red blood cell,FNRBC)数量的变化.方法 选择临床确诊的妊娠期高血压疾病患者30例(其中子痫前期轻度12例,子痫前期重度12例,子痫6例),妊娠期糖尿病患者23例(其中血糖控制理想无低血糖或酮症发生13例,血糖控制

  1. Prostaglandin I2 and Prostaglandin E2 Modulate Human Intrarenal Artery Contractility Through Prostaglandin E2-EP4, Prostacyclin-IP, and Thromboxane A2-TP Receptors

    DEFF Research Database (Denmark)

    Eskildsen, Morten P; Hansen, Pernille B L; Stubbe, Jane

    2014-01-01

    receptors. Intrarenal arteries were microdissected from human nephrectomy samples (n=53, median diameter ≈362 μm, 88% viable, 76% relaxed in response to acetylcholine). Rings were suspended in myographs to record force development. In vessels with K(+)-induced tension (EC70: -log [mol/L]=1.36±0.03), PGE2....../L elicited increased tension. This was abolished by thromboxane receptor (TP) antagonist (S18886, 10(-6) mol/L). A TP agonist (U46619, n=6) evoked tension (EC50=8.1±0.2) that was inhibited by S18886. Polymerase chain reaction and immunoblotting showed EP4, IP, and TP receptors in intrarenal arteries......Cyclooxygenase inhibitors decrease renal blood flow in settings with decreased effective circulating volume. The present study examined the hypothesis that prostaglandins, prostaglandin E2 (PGE2) and prostacyclin (PGI2), induce relaxation of human intrarenal arteries through PGE2-EP and PGI2-IP...

  2. Blood

    Science.gov (United States)

    ... Also, blood is either Rh-positive or Rh-negative. So if you have type A blood, it's either A positive or A negative. Which type you are is important if you need a blood transfusion. And your Rh factor could be important ...

  3. IDENTIFYING GENETIC ASSOCIATIONS WITH VARIABILITY IN METABOLIC HEALTH AND BLOOD COUNT LABORATORY VALUES: DIVING INTO THE QUANTITATIVE TRAITS BY LEVERAGING LONGITUDINAL DATA FROM AN EHR.

    Science.gov (United States)

    Verma, Shefali S; Lucas, Anastasia M; Lavage, Daniel R; Leader, Joseph B; Metpally, Raghu; Krishnamurthy, Sarathbabu; Dewey, Frederick; Borecki, Ingrid; Lopez, Alexander; Overton, John; Penn, John; Reid, Jeffrey; Pendergrass, Sarah A; Breitwieser, Gerda; Ritchie, Marylyn D

    2016-01-01

    A wide range of patient health data is recorded in Electronic Health Records (EHR). This data includes diagnosis, surgical procedures, clinical laboratory measurements, and medication information. Together this information reflects the patient's medical history. Many studies have efficiently used this data from the EHR to find associations that are clinically relevant, either by utilizing International Classification of Diseases, version 9 (ICD-9) codes or laboratory measurements, or by designing phenotype algorithms to extract case and control status with accuracy from the EHR. Here we developed a strategy to utilize longitudinal quantitative trait data from the EHR at Geisinger Health System focusing on outpatient metabolic and complete blood panel data as a starting point. Comprehensive Metabolic Panel (CMP) as well as Complete Blood Counts (CBC) are parts of routine care and provide a comprehensive picture from high level screening of patients' overall health and disease. We randomly split our data into two datasets to allow for discovery and replication. We first conducted a genome-wide association study (GWAS) with median values of 25 different clinical laboratory measurements to identify variants from Human Omni Express Exome beadchip data that are associated with these measurements. We identified 687 variants that associated and replicated with the tested clinical measurements at p<5×10-08. Since longitudinal data from the EHR provides a record of a patient's medical history, we utilized this information to further investigate the ICD-9 codes that might be associated with differences in variability of the measurements in the longitudinal dataset. We identified low and high variance patients by looking at changes within their individual longitudinal EHR laboratory results for each of the 25 clinical lab values (thus creating 50 groups - a high variance and a low variance for each lab variable). We then performed a PheWAS analysis with ICD-9 diagnosis codes

  4. RBC count

    Science.gov (United States)

    ... Failure of the right side of the heart ( cor pulmonale ) Dehydration (for example, from severe diarrhea) Kidney tumor ( ... Read More Alport syndrome Anemia CBC blood test Cor pulmonale Dehydration Erythropoietin test Folic acid in diet Glucose- ...

  5. Evaluation on clinical application of XE-2100 blood cell automatic analyzer for nucleated red blood cell count%XE-2100全自动血细胞分析仪对有核红细胞分析的应用评价

    Institute of Scientific and Technical Information of China (English)

    杨晓枫

    2013-01-01

    Objective To evaluate the veracity of nucleated red blood cell ( NRBC ) count by Sysmex XE - 2100 automatic blood cell analyzer. Methods The peripheral blood NRBC counts of 139 patients were examined by both Sysmex XE - 2100 automatic blood cell analyzer and microscopy. Results The counted results of high value and medium value had no significant difference between manual and automatic methods ( P 〉 0.05 ), the counted results of low value had significant difference between manual and automatic methods ( P 〈 0.05 ). The method by automatic analyzer had fine reproducibility. Conclusion The application of XE - 2100 automatic blood cell analyzer for NRBC count is quick and accurate , and it can provide accurate blood cell monitoring data for clinicians.%目的 评价Sysmex XE-2100全自动血细胞分析仪对有核红细胞(NRBC)分析结果的准确性.方法 采用仪器法和显微镜计数法分别对2006年1月至2011年2月间139例外周血NRBC阳性的临床血液样本进行测定.结果 仪器法对NRBC的检测结果与显微镜计数法比较,中、高值差异无统计学意义(P>0.05),低值差异有统计学意义(P<0.05).仪器的重复性良好.结论 Sysmex XE-2100全自动血细胞分析仪分析结果准确、快速,可为临床医生提供准确血细胞监测数据.

  6. Study on Relativity of Platelet Count of Platelet-rich Plasma and Platelet Count,Hematocrit of Whole Blood%富血小板血浆血小板浓度与全血血小板浓度和红细胞比容相关性探讨

    Institute of Scientific and Technical Information of China (English)

    李祖兰; 任军伟; 丛玉隆; 白洁; 邓新立; 马长生; 陈兴明; 杨亮程; 王海立

    2012-01-01

    目的 探讨富血小板血浆(PRP)血小板浓度与全血血小板浓度、红细胞比容(HCT)相关性.方法 随机收集162例门诊体检志愿者静脉血标本,以EDTA-K2,枸橼酸钠抗凝.枸橼酸钠抗凝血800 r/min(离心半径19 cm)离心5 min,分离富含血小板血浆(PRP),应用Sysmex XE-2100血液分析仪测定全血血小板浓度(X1)和HCT(X2),PRP血小板浓度(Y).以HCT 0.35为界,将数据分为正常组和低值组.结果 所得数据采用多元相关性统计分析得到回归方程Y总=1.309 51X1 +744.294 5X2-262.068(R2 =0.897 8);Y正常组=1.380 208X1 +855.884 8X2-323.374(R2=0.892 9);Y低值组=1.088 972X1 +465.228 8X2-123.101(R2=0.961 1).结论 全血血小板浓度、红细胞比容与富血小板血浆血小板浓度相关显著,由全血血小板浓度和红细胞比容可初步推算富血小板血浆血小板浓度.%Objective To explore relativity of platelet count of platelet-rich plasma, platelet count and hematocrit of whole blood. Methods Venous blood sample of outpatient volunteers were collected and anticoagulaged by EDTA-K2 and sodium citrate(n=162) ,and platelet-rich plasma(PRP) was obtained by centrifuging the blood anticoagulaged by sodium citrate at a low speed (800 r/min, r=19 cm) for 5 minute, then platelet number of PRP(Y) ,platelet number (X1 ) and hematocrit (X2) of whole blood were detected by Sysmex XE-2100 blood analyzer. The data was divided into normal and low group by 0. 35 of hematocrit. Results Regression equation analysed by multiple linear regression: Ytotal = 1.309 51X1 +744.294 5X2 - 262. 068(R2= 0.897 8) ;Ynormal= l. 380 208 X1+855. 884 8X2 -323. 374(R2 =0. 892 9);Ylow = l. 088 972X,+465. 228 8 X2 - 123. 101(R2 =0. 961 1). Conclusion Platelet count of the platelet-rich plasma,platelet count and hematocrit of the whole blood seem to relate significantly, and platelet count of platelet-rich plasma could be estimated approximately by platelet count and hematocrit of the whole blood.

  7. Counting Possibilia

    Directory of Open Access Journals (Sweden)

    Alfredo Tomasetta

    2010-06-01

    Full Text Available Timothy Williamson supports the thesis that every possible entity necessarily exists and so he needs to explain how a possible son of Wittgenstein’s, for example, exists in our world:he exists as a merely possible object (MPO, a pure locus of potential. Williamson presents a short argument for the existence of MPOs: how many knives can be made by fitting together two blades and two handles? Four: at the most two are concrete objects, the others being merely possible knives and merely possible objects. This paper defends the idea that one can avoid reference and ontological commitment to MPOs. My proposal is that MPOs can be dispensed with by using the notion of rules of knife-making. I first present a solution according to which we count lists of instructions - selected by the rules - describing physical combinations between components. This account, however, has its own difficulties and I eventually suggest that one can find a way out by admitting possible worlds, entities which are more commonly accepted - at least by philosophers - than MPOs. I maintain that, in answering Williamson’s questions, we count classes of physically possible worlds in which the same instance of a general rule is applied.

  8. Mechanisms Involved in Thromboxane A2 -induced Vasoconstriction of Rat Intracavernous Small Penile Arteries.

    Science.gov (United States)

    Grann, Martin; Comerma-Steffensen, Simon; Arcanjo, Daniel D R; Simonsen, Ulf

    2016-10-01

    Diabetes is associated with erectile dysfunction and with hypercontractility in erectile tissue and this is in part ascribed to increased formation of thromboxane. Rho kinase (ROCK) is a key regulator of calcium sensitization and contraction in vascular smooth muscle. This study investigated the role of calcium and ROCK in contraction evoked by activation of the thromboxane receptors. Rat intracavernous penile arteries were mounted for isometric tension and intracellular calcium ([Ca(2+) ]i ) recording and corpus cavernosum for measurements of MYPT1 phosphorylation. In penile arteries, U46619 by activation of thromboxane receptors concentration dependently increased calcium and contraction. U46619-induced calcium influx was blocked by nifedipine, a blocker of L-type calcium channels, and by 2-aminoethoxydiphenyl borate, a blocker of transient receptor potential (TRP) channels. Inhibitors of ROCK, Y27632 and glycyl-H1152P, concentration dependently reduced U46619-induced contraction, but only Y27632 reduced [Ca(2+) ]i levels in the penile arteries activated with either high extracellular potassium or U46619. MYPT-Thr(850) phosphorylation in corpus cavernous strips was increased in response to U46619 through activation of TP receptors and was found to be a direct result of phosphorylation by ROCK. Y27632 induced less relaxation in mesenteric arteries, H1152P induced equipotent relaxations, and a protein kinase C inhibitor, Ro-318220, failed to relax intracavernous penile arteries, but induced full relaxation in rat mesenteric arteries. Our findings suggest that U46619 contraction depends on Ca(2+) influx through L-type and TRP channels, and ROCK-dependent mechanisms in penile arteries. Inhibition of the ROCK pathway is a potential approach for the treatment of erectile dysfunction associated with hypertension and diabetes.

  9. Involvement of COX2-thromboxane pathway in TCDD-induced precardiac edema in developing zebrafish.

    Science.gov (United States)

    Teraoka, Hiroki; Okuno, Yuki; Nijoukubo, Daisuke; Yamakoshi, Ayumi; Peterson, Richard E; Stegeman, John J; Kitazawa, Takio; Hiraga, Takeo; Kubota, Akira

    2014-09-01

    The cardiovascular system is one of the most characteristic and important targets for developmental toxicity by 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) in fish larvae. However, knowledge of the mechanism of TCDD-induced edema after heterodimerization of aryl hydrocarbon receptor type 2 (AHR2) and AHR nuclear translocator type 1 (ARNT1) is still limited. In the present study, microscopic analysis with a high-speed camera revealed that TCDD increased the size of a small cavity between the heart and body wall in early eleutheroembryos, a toxic effect that we designate as precardiac edema. A concentration-response curve for precardiac edema at 2 days post fertilization (dpf) showed close similarity to that for conventional pericardial edema at 3 dpf. Precardiac edema caused by TCDD was reduced by morpholino knockdown of AHR2 and ARNT1, as well as by an antioxidant (ascorbic acid). A selective inhibitor of cyclooxygenase type 2 (COX2), NS398, also markedly inhibited TCDD-induced precardiac edema. A thromboxane receptor (TP) antagonist, ICI-192,605 almost abolished TCDD-induced precardiac edema and this effect was canceled by U46619, a TP agonist, which was not influential in the action of TCDD by itself. Knockdown of COX2b and thromboxane A synthase 1 (TBXS), but not COX2a, strongly reduced TCDD-induced precardiac edema. Knockdown of COX2b was without effect on mesencephalic circulation failure caused by TCDD. The edema by TCDD was also inhibited by knockdown of c-mpl, a thrombopoietin receptor necessary for thromobocyte production. Finally, induction of COX2b, but not COX2a, by TCDD was seen in eleutheroembryos at 3 dpf. These results suggest a role of the COX2b-thromboxane pathway in precardiac edema formation following TCDD exposure in developing zebrafish.

  10. Assumed white blood cell count of 8,000 cells/μL overestimates malaria parasite density in the Brazilian Amazon.

    Directory of Open Access Journals (Sweden)

    Eduardo R Alves-Junior

    Full Text Available Quantification of parasite density is an important component in the diagnosis of malaria infection. The accuracy of this estimation varies according to the method used. The aim of this study was to assess the agreement between the parasite density values obtained with the assumed value of 8,000 cells/μL and the automated WBC count. Moreover, the same comparative analysis was carried out for other assumed values of WBCs. The study was carried out in Brazil with 403 malaria patients who were infected in different endemic areas of the Brazilian Amazon. The use of a fixed WBC count of 8,000 cells/μL to quantify parasite density in malaria patients led to overestimated parasitemia and resulted in low reliability when compared to the automated WBC count. Assumed values ranging between 5,000 and 6,000 cells/μL, and 5,500 cells/μL in particular, showed higher reliability and more similar values of parasite density when compared between the 2 methods. The findings show that assumed WBC count of 5,500 cells/μL could lead to a more accurate estimation of parasite density for malaria patients in this endemic region.

  11. Weekly monitoring of the effects of conventional external beam radiation therapy on patients with head and neck, chest, and pelvis cancer by means of blood cells count

    Energy Technology Data Exchange (ETDEWEB)

    Lundgren, Maria da Salete Fonseca dos Santos [Hospital Universitario Oswaldo Cruz, Recife, PE (Brazil). Radiotherapy Unit]. E-mail: salete@lundgren.med.br; Cavalcanti, Maria do Socorro de Mendonca [Universidade de Pernambuco, Recife, PE (Brazil); Sampaio, Divaldo de Almeida [Centro de Hematologia de Pernambuco (Hemope), Recife, PE (Brazil)

    2008-01-15

    Objective: To evaluate the necessity of weekly monitoring by means of leukocyte and platelet counts of patients with head and neck, chest, and pelvis cancer submitted to conventional radiotherapy. Materials and methods: A hundred and one adult patients with cancer of head and neck (n = 11), chest (n = 35) and pelvis (n = 55), submitted to radiotherapy were assessed by means of leukocyte and platelet counts on a weekly basis, with a comparison between the results before and during the treatment and in correlation with the area treated, patient's sex and age group. Results: The most significant decrease in leukocytes was observed in the fourth week, when lymphocytes, total leukocytes, neutrophils, monocytes and platelets presented a decrease of 53.5%, 26.8%, 19.4%, 22.2% and 14.6%, respectively, in comparison with the values found before the beginning of the therapy. Geometric means for pelvis during the treatment were lower than those for chest, and head and neck. Lymphocytes demonstrated to be more sensitive to radiation therapy. No alteration was found in leukocyte or platelet counts in correlation with patients' sex or age. Conclusion: Based on the results of the present study, weekly leukocyte and platelet counts do not seem to be useful in the assessment patients submitted to conventional radiotherapy for localized cancer. (author)

  12. Effects of Agave tequilana fructans with different degree of polymerization profiles on the body weight, blood lipids and count of fecal Lactobacilli/Bifidobacteria in obese mice.

    Science.gov (United States)

    Márquez-Aguirre, Ana Laura; Camacho-Ruiz, Rosa Maria; Arriaga-Alba, Myriam; Padilla-Camberos, Eduardo; Kirchmayr, Manuel Reinhart; Blasco, José Luis; González-Avila, Marisela

    2013-08-01

    Fructans are dietary fibers with beneficial effects on the gastrointestinal physiology and offer a promising approach for the treatment of some metabolic disorders associated with obesity. In vitro and in vivo studies were developed to test the safety of fructans obtained from Agave tequilana Weber var. azul. Additionally, an in vivo experiment using a diet-induced obesity model was performed to compare the effect of agave fructans with different degree of polymerization (DP) profiles: agave fructans with DP > 10 (LcF), agave FOS with DP agave fructans with and without demineralization (dTF, TF) versus commercial chicory fructans (OraftiSynergy1™) on the body weight change, fat, total cholesterol, triglycerides and count of fecal Lactobacillus spp. and Bifidobacterium spp. Results showed that A. tequilana fructans were not mutagenic and were safe even at a dose of 5 g per kg b.w. Obese mice that received ScF showed a significant decrease in body weight gain, fat tissue and total cholesterol without increasing the count of fecal Bifidobacteria. Whereas, obese mice that received LcF and TF showed decreased triglycerides and an increased count of fecal Bifidobacteria. Interestingly, although obese mice that received dTF did not show changes in body weight gain, fat tissue, total cholesterol or triglycerides, they showed an increase in the count of Bifidobacteria. These results demonstrate that both the degree of polymerization and the demineralization process can influence the biological activity of agave fructans.

  13. Effects of the dual TP receptor antagonist and thromboxane synthase inhibitor EV-077 on human endothelial and vascular smooth muscle cells

    Energy Technology Data Exchange (ETDEWEB)

    Petri, Marcelo H. [Department of Medicine, Karolinska Institutet and Center for Molecular Medicine, Karolinska University Hospital, Stockholm (Sweden); Tellier, Céline; Michiels, Carine [NARILIS, URBC, University of Namur, Namur (Belgium); Ellertsen, Ingvill [Department of Medicine, Karolinska Institutet and Center for Molecular Medicine, Karolinska University Hospital, Stockholm (Sweden); Dogné, Jean-Michel [Department of Pharmacy, Namur Thrombosis and Hemostasis Center, University of Namur, Namur (Belgium); Bäck, Magnus, E-mail: Magnus.Back@ki.se [Department of Medicine, Karolinska Institutet and Center for Molecular Medicine, Karolinska University Hospital, Stockholm (Sweden)

    2013-11-15

    Highlights: •EV-077 reduced TNF-α induced inflammation in endothelial cells. •The thromboxane mimetic U69915 enhanced vascular smooth muscle cell proliferation. •EV-077 inhibited smooth muscle cell proliferation. -- Abstract: The prothrombotic mediator thromboxane A{sub 2} is derived from arachidonic acid metabolism through the cyclooxygenase and thromboxane synthase pathways, and transduces its effect through the thromboxane prostanoid (TP) receptor. The aim of this study was to determine the effect of the TP receptor antagonist and thromboxane synthase inhibitor EV-077 on inflammatory markers in human umbilical vein endothelial cells and on human coronary artery smooth muscle cell proliferation. To this end, mRNA levels of different proinflammatory mediators were studied by real time quantitative PCR, supernatants were analyzed by enzyme immune assay, and cell proliferation was assessed using WST-1. EV-077 significantly decreased mRNA levels of ICAM-1 and PTX3 after TNFα incubation, whereas concentrations of 6-keto PGF1α in supernatants of endothelial cells incubated with TNFα were significantly increased after EV-077 treatment. Although U46619 did not alter coronary artery smooth muscle cell proliferation, this thromboxane mimetic enhanced the proliferation induced by serum, insulin and growth factors, which was significantly inhibited by EV-077. In conclusion, EV-077 inhibited TNFα-induced endothelial inflammation and reduced the enhancement of smooth muscle cell proliferation induced by a thromboxane mimetic, supporting that the thromboxane pathway may be associated with early atherosclerosis in terms of endothelial dysfunction and vascular hypertrophy.

  14. Ethanol increase PGE and thromboxane production in mouse pregnant uterine tissue

    Energy Technology Data Exchange (ETDEWEB)

    Anton, R.F.; Becker, H.C.; Randall, C.L. (Veterans Administration Medical Center, Charleston, SC (USA))

    1990-01-01

    The teratogenic effect of ethanol in the C57BL/6J mouse can be attenuated by pretreatment with aspirin (ASA). One prominent effect of ASA is to inhibit prostaglandin (PGE) and thromboxane (TXB{sub 2}) production. We examined the effect in vivo ethanol exposure on PGE and TXB{sub 2} production in a uterine-embryo tissue sample of C57BL/6J mice either before or after in vivo ASA pretreatment on day 10 of gestation. Ethanol increase both PGE and TXB{sub 2} production by approximately 20%. ASA caused a marked reduction of PGE and TXB{sub 2} in both control and ethanol groups by approximately 80-90%. The mouse strain, gestation time, and study parameters used in this study were the same as in the previously reported ASA attenuation of the teratogenic effect of ethanol. Therefore, the present data add additional support to the hypothesis that prostaglandin and/or thromboxane production may be involved in at least some aspects of fetal alcohol syndrome.

  15. Effects of prostaglandins and thromboxane analogues on bullock and dog iris sphincter preparations.

    Science.gov (United States)

    Dong, Y J; Jones, R L

    1982-05-01

    1 The bullock iris sphincter was contracted by low concentrations of prostaglandin E2 (PGE2), 16, 16-dimethyl PGE2 and 17,18,19,20-tetranor-16-p-chlorophenoxy PGE2. Other compounds with thromboxane-like actions, for example 11,9-epoxymethano PGH2, were also potent spasmogens, ZK 36374, a stable carbacyclin, was a partial agonist on the PGE-sensitive system of this tissue. 2 The thromboxane antagonist, EP 045, had little effect on the action of PGE2 and 16,16-dimethyl PGE2 on the bullock iris. 3 The dog iris sphincter was sensitive to PGF2 alpha but not to PGE2 and 11,9-epoxymethano PGH2. 4 16,16-dimethyl PGE2 had very low activity on the dog iris in contrast to its high activity on the bullock iris. The reverse was found with the 17,18,19,20-tetranor-16-m-trifluoromethylphenoxy analogue of PGF2 alpha (ICI 81008). This indicates a considerable selectivity of action of the two analogues. 5 The results are discussed in relation to the existing knowledge of prostanoid receptors.

  16. Additive effect of cysteinyl leukotriene or thromboxane modifiers to inhaled corticosteroids in asthmatic patients

    Directory of Open Access Journals (Sweden)

    Shigeharu Myou

    2004-01-01

    Full Text Available Bronchial asthma is a chronic inflammatory disease of the conducting airways. Current asthma treatment guidelines recommend inhaled corticosteroids (ICS as the first-line maintenance therapy for mild to severe persistent asthma, because ICS are the most efficacious anti-inflammatory medication. Despite treatment with ICS, suppression of inflammation is often incomplete and blockade by ICS of cysteinyl leukotriene (CysLT and thromboxane (TX A2 biosynthesis is limited. The addition of a CysLT1 receptor antagonist to an ICS represents a reasonable alternative therapeutic approach for the treatment of asthma patients whose symptoms remain uncontrolled on ICS alone. Indeed, CysLT1 receptor antagonists are demonstrated both to have an additive effect to ICS therapy and to allow the reduction of ICS dosage. Thromboxane modifiers also have an additive effect with low- to moderate-dose ICS. Although the long-term usefulness of add-on therapy of CysLT or TX modifiers (vs long-acting β2-adrenergic receptor agonists to ICS is unclear, these alternatives are worthy of further consideration.

  17. Tritium labeled amino acid conjugates of prostaglandins and thromboxanes as labeled ligands in prostanoid radioimmunoassay

    Energy Technology Data Exchange (ETDEWEB)

    Sautebin, L.; Kindahl, H.; Kumlin, M.; Granstroem, E.

    1985-09-01

    Conjugates of prostaglandins and thromboxanes with tritium labeled amino acids were prepared and employed as labeled ligands in prostaglandin and thromboxane radioimmunoassays. Assays for PGF2 alpha, 15-keto-13, 14-dihydro-PGF2 alpha, TXB2 and 15-keto-13,14-dihydro-TXB2 were evaluated in comparative studies using either these heterologous ligands or the corresponding homologous tritiated eicosanoid as tracers. Binding properties for the respective antibodies were found to be similar using either tracer. Three biological studies were also conducted, viz. study of the release of TXB2 during collagen induced platelet aggregation, of 15-keto-13,14-dihydro-TXB2 during guinea pig pulmonary anaphylaxis, and of PGF2 alpha during bovine luteolysis. The analyses gave comparable results using either the heterologous or the homologous assay. Thus, this type of labeled prostanoid conjugates may serve as a convenient alternative to homologous tracers in radioimmunoassay. Heterologous tracers may even in certain cases provide the only simple solution to the problem of preparing a labeled ligand of high specific activity.

  18. The investigation and analyses on blood count of the part of officers and soldiers from Zhoushan district%舟山地区部分部队官兵血常规的调查分析

    Institute of Scientific and Technical Information of China (English)

    向阳; 王晓波; 宋伟娜; 常晓慧; 刘晓宇; 王伟; 杜欣

    2012-01-01

    目的 调查舟山地区部分部队官兵血常规的异常检出情况.方法 采用整群抽样方法,采集444名陆海军官兵的静脉血2ml,检测白细胞(WBC)、红细胞(RBC)、血红蛋白(Hb)、血小板(BPC).结果 ①受检官兵的WBC、RBC、Hb、BPC分别为(5.9±1.4)× 109/L、(5.2±0.4)×1012/L、(144.4±12.1)g/L、(243.9±47.4)× 109/L;②WBC、RBC、Hb和BPC异常检出率分别为8.0%、17.6%、7.5%和11.7%,其中RBC异常检出率最高,其次为WBC、BPC;③海军官兵4项指标的异常检出率均高于陆军,其中Hb的异常检出率的差异有统计学意义(P<0.05).结论 舟山地区受检官兵WBC、RBC、Hb和BPC等4项血常规的主要指标均在正常范围,对于异常检出率应采取措施予以动态观察、及时诊治.%Objective To investigate and analyzing the abnormal detection condition on blood count in the part of officers and soldiers from Zhoushan district. Methods The cluster sampling was used. About 2 ml of venous blood were individually collected from 444 officers and soldiers in army and navy. White blood cell count( WBC) ,red blood cell count( RBC) ,hemoglobin(Hb) and blood platelet count( BPC) were detected. Results Among 444 officers and soldiers,WBC,RBC,Hb,BPC were(5.9 ±1.4) × 109/L, (5. 2 ±0.4) × 1012/ L,( 144.4 ±12. l)g/L,(243.9±47.4) X lO'/L respectively. The abnormality detection rates of WBC,RBC, Hb,and BPC were 8. 0% , 17. 6% ,7.5% and 11.7% respectively. RBC was the highest and the higher was WBC and BPC. Each abnormality detection rate of 4 indexes from navy was higher than that of army,but the difference of Hb only had statistical significance ( P < 0. 05). Conclusion The chief indexes of blood count such as WBC,RBC,Hb,and BPC of the officers and 9oldiers can maintain normal level. The measures should be adopted for abnormality detection rate with dynamic status in order to diagnose and treat in time.

  19. Incidence of spontaneous bacterial peritonitis in patients with ascites. Diagnostic value of white blood cell count and pH measurement in ascitic fluid

    DEFF Research Database (Denmark)

    Storgaard, J S; Svendsen, Jesper Hastrup; Hegnhøj, J

    1991-01-01

    During a 21-month period, 65 consecutive patients admitted with ascites were included in a prospective study of the incidence of spontaneous bacterial peritonitis, and paracentesis was performed on admission. The ascitic fluid was cultured, ascitic leucocytes were counted and pH was measured....... Bacterial growth was found in five patients with chronic liver disease, who were diagnosed as having spontaneous bacterial peritonitis (SBP), since no intra-abdominal focus could be demonstrated. Thus, the incidence of SBP in this material was 7.7% (95% confidence limits: 2.5-17%). SBP was caused...

  20. Elevated neutrophil and monocyte counts in peripheral blood are associated with poor survival in patients with metastatic melanoma: a prognostic model

    DEFF Research Database (Denmark)

    Schmidt, Henrik; Bastholt, Lars; Geertsen, Poul;

    2005-01-01

          treated as part of several phase II protocols and the majority received       treatment with intermediate dose subcutaneous IL-2 and interferon-alpha.       Neutrophil and monocyte counts, lactate dehydrogenase (LDH), number of       metastatic sites, location of metastases and performance status were all......       statistically significant prognostic factors in univariate analyses.       Subsequently, a multivariate Cox's regression analysis identified elevated       LDH (P

  1. Relationship of prognosis and body temperature and white blood cell count in peripheral blood in early stage of patients of cerebral hemorrhage%急性脑出血患者早期体温变化及外周血白细胞计数与预后的关系

    Institute of Scientific and Technical Information of China (English)

    杨春永; 孙京莉

    2011-01-01

    目的 探讨急性脑出血患者早期体温变化及外周血白细胞计数与预后的关系.方法 将77例脑出血患者按体温与外周血白细胞计数分别分成发热组与不发热组;白细胞数正常组与白细胞数增高组;白细胞数轻度增高组与显著增高组,观察各组死亡率并进行统计学分析.结果 发热组死亡率(67.3%)高于不发热组的死亡率(20.0%)(P<0.01);白细胞数正常组死亡率(32.4%)低于白细胞数增高组的死亡率(67.4%)(P<0.01);白细胞数轻度增高组死亡率(43.5%)低于显著白细胞数增高组的死亡率(95.0%)(P<0.01).结论 根据急性脑出血患者早期体温变化及外周血白细胞计数可对预后做出较为准确的判断,且方法简单、易行,有很高的临床应用价值.%Objective To investigate the relationship of prognosis and body temperature and white blood cell count in peripheral blood in early stage of patients of cerebral hemorrhage. Methods Seventy-seven patients of cerebral hemorrhage were divided into fever group and normal temperature group, white blood cell count increasing group and normal white blood cell count group, white blood cell minimal increasing group and white blood cell increasing significantly group. Mortality of each group were calculated and compared. Results The mortality of fever group and normal temperature group was 67. 3% and 20.0% . There was significant difference between two groups ( P < 0. 01). The mortality of white blood cell count increasing group and normal white blood cell count group was 32.4% and 67.4%. There was significantly difference between two group ( P < 0.01). The mortality of white blood cell minimal increasing group and white blood cell increasing significantly group was 43. 5% and 95.0%. There was significantly difference in two group (P <0.01). Conclusion It would determine the prognosis by study body temperature and white blood cell count in peripheral blood in early stage of

  2. Evidence that the angiotensin at 2-receptor agonist compound 21 is also a low affinity thromboxane TXA2-receptor antagonist

    DEFF Research Database (Denmark)

    Fredgart, M.; Leurgans, T.; Stenelo, M.;

    2015-01-01

    Objective: The objective of this study was to test whether Compound 21 (C21), a high-affinity, non-peptide angiotensinAT2-receptor agonist, is also an antagonist of thromboxane A2 (TXA2) receptors thus reducing both vasoconstriction and platelet aggregation. Design and method: Binding of C21 to t...

  3. 手足口病患儿心肌酶谱和全血细胞计数变化分析%Change analysis of myocardial enzyme spectrum and complete blood count of children with hand, foot and mouth disease

    Institute of Scientific and Technical Information of China (English)

    郑明江

    2014-01-01

    Objective To study change of myocardial enzyme spectrum and complete blood count of children with hand, foot and mouth disease. Methods 60 children with hand, foot and mouth disease were selected by sampling as the research objects, and set as observation group. 60 healthy children were selected as control group at the same period. Serum AST, LDH, CK, CK-MB, HBDH and CBC content changes of children of two groups were compared. Results Neutrophils, basophil, acidophil counting contents of children of two groups were of no significant difference, and there was no statistically significant difference (P>0.05). But count contents of white blood cells, lymphatic lymph and mononuclear cell in observation group increased significantly, and compared with control group, there was statistically significant difference (P0.05)。但是观察组患儿的白细胞、淋巴细胞、单核细胞计数含量却明显升高,与对照组相比差异有统计学意义(P<0.05)。观察组患儿的AST、LDH、CK、CK-MB、HBDH含量明显升高,相比对照组健康小儿,差异有统计学意义(P<0.05)。结论心肌酶谱和全血细胞计数变化对于手足口病患儿的诊断和预后极具指导价值。

  4. Significance of peripheral blood fibrocytes counting to renal fibrosis rats%肾脏纤维化大鼠外周血纤维细胞计数变化及意义

    Institute of Scientific and Technical Information of China (English)

    党艳梅; 李新建; 张少青; 曲小菡; 魏明明; 张昆

    2015-01-01

    目的:通过流式细胞仪对5/6肾切除大鼠外周血中的纤维细胞计数变化,观察外周血纤维细胞数量与肾脏纤维化程度之间的关系,探讨外周血纤维细胞作为肾纤维化早期生物学标志物的可行性。方法清洁级雄性SD大鼠10只,随机分为对照组(n=4)和模型组(n=6)。模型组5/6肾切除制作肾脏纤维化模型,对照组只剥离肾包膜,不切除肾实质。以CD45和Ⅰ型胶原作为循环纤维细胞的表面标记,使用流式细胞仪对两组大鼠外周血纤维细胞计数;结合肾功能生化指标及肾脏病理学改变,分析循环纤维细胞计数在肾纤维化中的意义。结果造模后第4、8、12周,模型组肾小球积分、肾小管间质积分、血清肌酐、尿素氮均高于对照组( P均<0.01)。模型组在造模后的第4、8、12周,外周血纤维细胞计数显著升高,高于对照组( P均<0.01)。外周血纤维细胞计数与血清肌酐、尿素氮呈正相关( r分别为0.623、0.550,P均<0.05)。结论外周血纤维细胞计数在肾纤维化早期与肾脏病理改变一致,可早期预示肾纤维化的发展进程。%Objective To discuss the significance of peripheral blood fibrocytes counting to renal fibrosis rats with 5/6 kidney cut .Methods CD45 and type I collagen were as the circulation fibrocytes surface markers; the flow cytometer was used to count the peripheral blood fibrocytes of rats with 5/6 kidney cut;biochemical indexes of renal function and the pathological alteration in kidney were measured .Results For the model group of rats with renal failure , in 4, 8 and 12 weeks after modeling , the peripheral blood fibrocytes counting increased significantly compared with the control group ( all P<0.01).The blood fibrocytes counting was positively correlated with renal pathology changes , Scr and BUN (P<0.05 or P<0.01).Conclusion The peripheral blood fibrocytes counting is

  5. Influence of danazol on blood platelet counts of ITP patients%达那唑对ITP患者血小板数量的影响

    Institute of Scientific and Technical Information of China (English)

    蔡艳

    2012-01-01

    目的 观察达那唑对血小板减少性紫癜(ITP)患者血小板数量的影响,并探讨其机制.方法 回顾性分析31例ITP患者,以达那唑为主进行治疗,疗程为3个月,并于治疗前、后行血小板计数检测及疗效判定.结果 治疗后,完全反应17例,部分反应7例,微小反应5例,无反应2例,总有效率为93%.患者的血小板数目提升.结论 达那唑治疗ITP的临床疗效满意,能有效提升患者血小板数量.%Objective To observe the effect of danazol on platelet count of thrombocytopenic purpura pa tients, and investigate the mechanism. Methods 31 cases of ITP patients were analyzed retrospectively. The treatment ( main with danazol ) lasted for 3 months,the platelet counts and efficacy were determined. Results After treatment, there were 17 cases of complete response,7 cases of partial response,5 cases of minor response,2 cases without re sponse, the total effective rate was 93 % . The number of platelet was improved. Conclusion The effect of danazol on ITP is good,danazol can effectively increase the number of platelets of ITP patients.

  6. Mechanisms Involved in Thromboxane A2-induced Vasoconstriction of Rat Intracavernous Small Penile Arteries

    DEFF Research Database (Denmark)

    Grann, Martin; Comerma Steffensen, Simon Gabriel; Arcanjo, Daniel Dias Rufino

    2015-01-01

    the role of calcium and ROCK in contraction evoked by activation of the thromboxane receptors. Rat intracavernous penile arteries were mounted for isometric tension and intracellular calcium ([Ca2+]i) recording and corpus cavernosum for measurements of MYPT1 phosphorylation. In penile arteries, U46619......27632 and glycyl-H1152P, concentrationdependently reduced U46619-induced contraction, but only Y27632 reduced [Ca2+]i-levels in the penile arteries activated with either high extracellular potassium or U46619. MYPTThr850 phosphorylation in corpus cavernous strips was increased in response to U46619...... through activation of TP receptors and was found to be a direct result of phosphorylation by ROCK. Y27632 induced less relaxation in mesenteric arteries, H1152P induced equipotent relaxations, and a protein kinase C inhibitor, Ro-318220, failed to relax intracavernous penile arteries, but induced full...

  7. Hypoxia increases pulmonary arterial thromboxane receptor internalization independent of receptor sensitization.

    Science.gov (United States)

    Fediuk, J; Sikarwar, A S; Lizotte, P P; Hinton, M; Nolette, N; Dakshinamurti, S

    2015-02-01

    Persistent Pulmonary Hypertension of the Newborn (PPHN) is characterized by sustained vasospasm and an increased thromboxane:prostacyclin ratio. Thromboxane (TP) receptors signal via Gαq to mobilize IP3 and Ca(2+), causing pulmonary arterial constriction. We have previously reported increased TP internalization in hypoxic pulmonary arterial (PA) myocytes. Serum-deprived PA myocytes were grown in normoxia (NM) or hypoxia (HM) for 72 h. TP localization was visualized in agonist-naïve and -challenged NM and HM by immunocytochemistry. Pathways for agonist-induced TP receptor internalization were determined by inhibiting caveolin- or clathrin-mediated endocytosis, and caveolar fractionation. Roles of actin and tubulin in TP receptor internalization were assessed using inhibitors of tubulin, actin-stabilizing or -destabilizing agents. PKA, PKC or GRK activation and inhibition were used to determine the kinase responsible for post-agonist receptor internalization. Agonist-naïve HM had decreased cell surface TP, and greater TP internalization after agonist challenge. TP protein did not sort with caveolin-rich fractions. Inhibition of clathrin prevented TP internalization. Both actin-stabilizing and -destabilizing agents prevented TP endocytosis in NM, while normalizing TP internalization in HM. Velocity of TP internalization was unaffected by PKA activity, but PKC activation normalized TP receptor internalization in HM. GRK inhibition had no effect. We conclude that in hypoxic myocytes, TP is internalized faster and to a greater extent than in normoxic controls. Internalization of the agonist-challenged TP requires clathrin, dynamic actin and is sensitive to PKC activity. TP receptor trafficking and signaling in hypoxia are pivotal to understanding increased vasoconstrictor sensitivity.

  8. Differential expression of thromboxane synthase in prostate carcinoma: role in tumor cell motility.

    Science.gov (United States)

    Nie, Daotai; Che, Mingxin; Zacharek, Alex; Qiao, Yan; Li, Li; Li, Xinglin; Lamberti, Mario; Tang, Keqin; Cai, Yilong; Guo, Yande; Grignon, David; Honn, Kenneth V

    2004-02-01

    Arachidonic acid metabolism through cyclooxygenase, lipoxygenase, or P-450 epoxygenase pathways can generate a variety of eicosanoids. Thromboxane synthase (TxS) metabolizes the cyclooxygenase product, prostanglandin H(2), into thromboxane A(2) (TXA(2)), which can cause vessel constriction, platelet activation, and aggregation. Here we demonstrate that human prostate cancer (PCa) cells express enzymatically active TxS and that this enzyme is involved in cell motility. In human PCa cell lines, PC-3, PC-3M, and ML-2 cells expressed higher levels of TxS than normal prostate epithelial cells or other established PCa cell lines such as DU145, LNCaP, or PPC-1. We cloned and sequenced the full-length TxS cDNA from PC-3 cells and found two changes in the amino acid residues. Immunohistochemical analysis of tumor specimens revealed that expression of TxS is weak or absent in normal differentiated luminal, or secretory cells, significantly elevated in less differentiated or advanced prostate tumors, and markedly increased in tumors with perineural invasion. TxS expressed in PC-3 cells was enzymatically active and susceptible to carboxyheptal imidazole, an inhibitor of TxS. The biosynthesis of TXA(2) in PC-3 cells was dependent on COX-2, and to a lesser extent, COX-1. Treatment of PC-3 cells with a COX-1 selective inhibitor, piroxicam, reduced TXA(2) synthesis by approximately 40%, while the COX-2 specific inhibitor NS398 reduced TXA(2) production by approximately 80%. Inhibition of TxS activity or blockade of TXA(2) function reduced PC-3 cell migration on fibronectin, while having minimal effects on cell cycle progression or survival. Finally, increased expression of TxS in DU145 cells increased cell motility. Our data suggest that human PCa cells express TxS and that this enzyme may contribute to PCa progression through modulating cell motility.

  9. Research on the calibration method of reticulocyte count interfered by plasmodium in red blood cells%红细胞内疟原虫干扰网织红细胞计数纠正方法的研究

    Institute of Scientific and Technical Information of China (English)

    孙海芳; 王志军; 吴凌燕

    2011-01-01

    目的:解决血液标本中红细胞内疟原虫对网织红细胞(Reticulocyte,Ret)计数结果的干扰问题,对网织红细胞检测结果进行校准.方法:通过两种不同的染色方法,一部分标本用新亚甲蓝或煌焦油蓝染液对血液常规标本进行活体染色后,采用手工或血液细胞分析仪对其进行网织红细胞计数;另一部分制成血涂片,使用瑞姬氏染色后,对含疟原虫的红细胞进行计数(与Ret的手工计数方法相同),含疟原虫红细胞%=1000个红细胞中含有疟原虫的红细胞个数/10.Ret的校正公式:Ret#(绝对数)校正值(×1012/L)=校正前Ret#一红细胞绝对数×含疟原虫的红细胞%,或Ret%(百分率)校正值:校正前Ret%-含疟原虫的红细胞%.结果:红细胞内疟原虫含RNA与网织红细胞内残存的RNA相似,经活体染色后,仪器和手工计数难以分辨,使Ret不同程度假性增高,10例疟疾患者set(%)的均值±标准差(±8)校正前后分别为9.86±13.89和6.63±10.93,Ret#(×1012/L)校正前后分别为0.35±0.47和0.09±0.11.疟疾患者的Ret结果受干扰的程度与含疟原虫红细胞数量有关,含疟原虫红细胞的百分率越高,干扰越大.结论:红细胞内疟原虫常可引起Ret假性增高,且增高程度极大,需要对Ret的结果进行校正.该方法具有简便、实用和科学的特点,通过该校正方法对红细胞内疟原虫干扰Ret检测的结果的校正,能够使仪器检测Ret结果更加准确可靠.%Objective:To solve the problem that plasmodium in red blood cells (RBC) interfere the reticulocyte (Ret) count and to calibrate the results.Methods: Samples were stained by two different dyeing methods.One method was that blood sample stained by methylene blue or brilliant cresol blue, then reticulocytes were counted by blood cell analyzer or manually.The other was that blood smears were stained by Wright - Giemsa and RBCs contained with plasmodium were counted with plasmodium in 1000RBCs/10.Calibration

  10. Effect of chronic alcohol consumption on the red blood cell count and RBC indices in the HIV infected patients on d4T/3TC/NVP drug regimen in Uganda

    Directory of Open Access Journals (Sweden)

    Godfrey S. Bbosa

    2013-10-01

    Full Text Available Alcohol consumption is common problem in Uganda. Among the types of alcohols consumed include beers, spirits, liqueurs, wines and traditional brew. These alcohols are easily accessible and consumed by many people including the HIV infected patients who are on the d4T/3TC/NVP regimen. The aim of this study was to determine the effect of chronic alcohol intake on the red blood cell count (RBC and the RBC indices in the HIV-infected patients on d4T/3TC/NVP regimen. It was a case control study that used a repeated measures design model where serial measurements of the red blood cell count (RBC and RBC indices were determined at 3 month interval for 9 months. A total of 41 HIV infected patients were recruited and grouped into two arms; the control group had 21 patients and the chronic alcohol group had 20 patients. The RBC and RBC indices of the whole blood were determined using automated hematological Coulter CBC-5 Hematology Analyzer system using standard procedures. The data was sorted into alcohol-use self reporting by WHO AUDIT tool and alcohol-use biomarkers groups. It was analysed using the SAS 2003 version 9.1 statistical package with the repeated measures fixed model. The means were compared using the student t-test. The mean MCV and MCH values in the chronic alcohol use group were higher than in the control group and there was a significant difference between the 2 groups (p<0.05 for both the WHO AUDIT tool group and chronic alcohol use biomarkers group. The mean RBC count, Hct, HGB and MCHC values in both the control and chronic alcohol use groups were within the normal reference ranges for both groups though the trend was lower in alcohol group. Chronic alcohol use affects the RBC and RBC indices in the HIV infected patients on d4T/3TC/NVP treatment regimen. [Int J Basic Clin Pharmacol 2013; 2(5.000: 528-536

  11. 献血者血小板数量及血容量对机采血小板采集量的影响%The Influence on Apheresis Platelets Collection Quantity of Platelets Counts and Blood Volume of Donors

    Institute of Scientific and Technical Information of China (English)

    蒋靓; 曹维娟; 王明元

    2014-01-01

    目的:分析献血员血小板数量及处理血量对机采血小板采集量的影响。方法应用Trima血细胞分离机采集100名献血者的双份血小板。结果通过多元回归分析发现,献血者采前的血小板计数(Plt)和血容量(BV)与血小板采集量之间存在回归关系,其标准偏回归系数β值分别为-0.370和-0.201,P<0.01。结论对采前的Plt和BV水平较低的献血者,可采用Trima血细胞分离机进行采集。%Objective To analyze the influence on apheresis platelets collection quantity of platelets count (Plt) and blood volume (BV) of donors. Methods Double platelets were collected from 100 donors using the Trima blood separator. Results By multivariate linear regression analysis, it was found that there were regression relationships between donors' Plt and BV before donation and platelets collection quantity (β=-0.370 and-0.201, P<0.01). Conclusion For donors with low level Plt or BV, Trima blood separator can be used.

  12. Experimental infection of commercial layers with wild or attenuated Salmonella Gallinarum mutant strains: anatomic pathology, total blood cell count and serum protein levels

    Directory of Open Access Journals (Sweden)

    KO Garcia

    2013-06-01

    Full Text Available The aim of the present study was to comparatively evaluate hemogram, blood serum components and anatomopathologic alterations in commercial layers experimentally challenged with an attenuated vaccine candidate strain (SG∆cobS∆cbiA and other two pathogenic strains (SGDcobS and SGNalr of Gallinarum (SG. In total, 280 commercial layers were randomly divided into 4 groups (G1, G2, G3 and G4. At five days of age, birds from groups G1 received approximately 107 colony forming units (CFU of SGDcobS; meanwhile birds from group G2 and G3 received the same dose of SGNalr and SG∆cobS∆cbiA, respectively. Birds from G4 were not infected. At 24 hours before (DBI and 24 hours after (1 DAI, and three (3 DAI, five (5 DAI, seven (7 DAI ten (10 DAI, and fifteen (15 DAI days after the infection, 10 birds of each group were humanely killed and blood samples collected to hematological and serum tests. Samples of liver, spleen, thymus, bursa of Fabricius, kidney and heart were also collected for the histological examination. Birds inoculated with SGDcobS and SGNalr showed similar alterations in hemogram, blood serum components and anatomopathologic exams. On the other hand, the exams of birds inoculated with SG∆cobS∆cbiA strain were similar to those of the uninfected birds. However, changes could be noticed in levels of uric acid and cholesterol during the course of the infection of birds from G3. Decrease in levels of light IgG 3 DAI was also observed in birds from this group. Pyknosis in kidney cells was a microscopic alteration found in birds from G3. Further studies must be done to verify if these alterations will not interfere in the performance of the vaccinate birds with SG∆cobS∆cbiA strain.

  13. Application of Whole Blood C-reactive Protein and White Blood Cell Count in Children With Pneumonia%全血C-反应蛋白与白细胞计数联合检测在小儿肺炎中的应用

    Institute of Scientific and Technical Information of China (English)

    温朝辉

    2016-01-01

    Objective To analyze the application of the detection of whole blood C-reactive protein and white blood cell count in infantile pneumonia.Methods 120 cases of children with pneumonia in our hospital from August 2013 to July 2015 were selected, there were 70 cases of bacterial bronchopneumonia and 50 cases of viral pneumonia among them. To selected another 60 healthy cases, to compare the whole blood C-reactive protein and white blood cell count about two groups.ResultsThe whole blood C-reactive protein and white blood cell count of the children with bacterial bronchopneumonia was higher than the children with viral pneumonia and the healthy cases, the difference was statistically significant (P<0.05). The whole blood C-reactive protein and white blood cell count of the children with the viral pneumonia was signiifcantly was higher than the healthy cases, the difference was statistically signiifcant (P<0.05).Conclusion The combination of whole blood C- reactive protein and white blood cell count can identify the type of pneumonia in children with pneumonia, and can detect and conifrm the bacterial bronchopneumonia faster.%目的:分析全血C-反应蛋白与白细胞计数联合检测在小儿肺炎中的应用。方法选取2013年8月~至2015年7月本院收治的肺炎患儿120例,其中细菌性支气管肺炎70例,病毒性肺炎50例,另再选取60例健康儿童,观察比较三组患儿的全血C-反应蛋白与白细胞计数。结果细菌性支气管肺炎患儿的全血C-反应蛋白和白细胞计数显著高于病毒性肺炎及健康儿童,差异具有统计学意义(P<0.05);病毒性肺炎患儿的全血C-反应蛋白与白细胞计数高于健康儿童,差异有统计学意义(P<0.05)。结论全血C-反应蛋白与白细胞计数联合检测在小儿肺炎中可以鉴别肺炎类型,能更快的检测及确诊出细菌性支气管肺炎。

  14. 双份机采血小板采集前后血常规结果分析%Study on the changes of the donor's blood cell counts before and after double apheresis platelets

    Institute of Scientific and Technical Information of China (English)

    伍娟; 孙革; 庄乃保; 熊恺轩; 王霞; 孙雄飞

    2015-01-01

    Objective To study the changes of the donor's blood routine indexes before and after double apheresis platelets, and then to explore the efficiency and the safety of double apheresis platelets. Methods The blood cell counts of 50 donors were examined by blood counting instrument before and after double apheresis plate-lets, and the data was analyzed on Paired Samples t-Test. Results After double apheresis platelets, PLT was signifi-cantly lower than that before double apheresis platelets (P0.05). And examination confirmed that the quality of the apheresis platelets products was up to specification, with the amount of platelets reaching 250 × 109/L and the qualification rate of 100%. Conclusion Double apheresis platelets can improve the efficiency and safety of platelets donation, and it would not affect the blood routine indexes of the donors.%目的:通过分析捐献双份机采血小板捐献者捐献前后血常规参数的变化,评价捐献双份机采血小板的效率和安全性。方法随机抽取本中心双份机采血小板捐献者50例,应用血细胞计数仪分别检测其献血前后血常规主要参数水平,并进行配对t检验。结果献血前与献血后PLT计数比较差异有统计学意义(P0.05),采集的血小板均能达到250×109/L,机采血小板合格率为100%,献血者无不适反应。结论双份机采血小板可以提高血小板采集效率,但不会明显影响捐献者血常规各主要参数的水平,机采双份血小板对捐献者不会有明显影响。

  15. Post hoc analysis of the relationship between baseline white blood cell count and survival outcome in a randomized Phase III trial of decitabine in older patients with newly diagnosed acute myeloid leukemia

    Directory of Open Access Journals (Sweden)

    Arthur C

    2015-01-01

    Full Text Available Christopher Arthur,1 Jaroslav Cermak,2 Jacques Delaunay,3 Jirí Mayer,4 Grzegorz Mazur,5 Xavier Thomas,6 Agnieszka Wierzbowska,7 Mark M Jones,8 Erhan Berrak,8 Hagop Kantarjian9 1Department of Haematology, Royal North Shore Hospital, Sydney, NSW, Australia; 2Institute of Hematology and Blood Transfusion, Prague, Czech Republic; 3Department of Clinical Hematology, University of Nantes, Nantes, France; 4Department of Internal Medicine, Masaryk University Hospital Brno, Central European Institute of Technology, Brno, Czech Republic; 5Department of Hematology, Wroclaw Medical University, Wroclaw, Poland; 6Department of Hematology, Edouard Herriot Hospital, Lyon, France; 7Copernicus Memorial Hospital, Lodz, Poland; 8Oncology Product Creation Unit, Eisai Inc., Woodcliff Lake, NJ, USA; 9Department of Leukemia, University of Texas MD Anderson Cancer Center, Houston, TX, USA Background: In a Phase III trial, 485 patients (≥65 years with newly diagnosed acute myeloid leukemia received decitabine 20 mg/m2 intravenously for 5 days every 4 weeks or a treatment choice (supportive care or cytarabine 20 mg/m2 subcutaneously for 10 days every 4 weeks. Materials and methods: We summarized overall and progression-free survival by baseline white blood cell count using two analyses: <1, 1–5, >5×109/L; ≤10 or .10×109/L. Results: There were 446 deaths (treatment choice, n=227; decitabine, n=219. Median overall survival was 5.0 (treatment choice versus 7.7 months (decitabine; nominal P=0.037. Overall survival differences between white blood cell groups were not significant; hazard ratios (HRs favored decitabine. Significant progression-free survival differences favored decitabine for groups 1–5×109/L (P=0.005, HR =0.67, greater than 5×109/L (P=0.027, HR =0.71, and up to 10×109/L (P=0.003, HR =0.72. Conclusion: There was a trend toward improved outcome with decitabine, regardless of baseline white blood cell count. Keywords: decitabine, acute myeloid

  16. Changes in monocyte counts and expression of mCD14 and HLA-DR in the peripheral blood of patients with severe acute respiratory syndrome

    Institute of Scientific and Technical Information of China (English)

    National Research Project for SARS, Beijing Group

    2004-01-01

    @@ Severe acute respiratory syndrome (SARS) is an infectious disease that originally emerged in China in November 2002. It subsequently spread worldwide.Investigators involved in an international collaboration have attempted to determine a specific etiology in order to redefine what is currently best described as a syndrome into a specific disease. At present, a novel coronavirus is generally accepted as the single most probable causative agent. In the case of HIV infection, monocytes/macrophages are infected early in the infection process,and the activation of monocytes/macrophages can influence the susceptibility of these cells to infection. 1Therefore, we examined the number of monocytes and the expression of CD14 and HLA-DR in the peripheral blood of patients with SARS to determine whether monocytes were involved in the pathogenesis of SARS.

  17. Changes in the concentration of hemoglobin, the count of erythrocytes and hematocrit of blood in conservation%血液保存中血红蛋白、红细胞和红细胞压积参数变化

    Institute of Scientific and Technical Information of China (English)

    邓梅英; 宁芳; 蒋利星

    2012-01-01

    目的 观察血液在不同保存时间内血红蛋白(Hb)、红细胞(RBC)和红细胞压积(HCT)含量的变化.方法 取2008年5月~2009年2月264份库存红细胞悬液的血标本,分别于6个时间段(4℃贮存0、7、14、21、28、35 d)测定血红蛋白浓度、红细胞计数和红细胞压积比值.结果 血液在保存7、14、21 d,其血红蛋白、红细胞和红细胞压积比值变化并不明显;而血液在保存了28、35 d后的血红蛋白、红细胞和红细胞压积比值与前三组结果比较明显的下降.Hb、RBC 、HCT三项检测指标经t检验,差异有统计学意义(P<0.05).结论 血液在体外保存过程中,红细胞的数量、血红蛋白浓度和红细胞压积比值随着血液保存的时间延长而减少和降低,尤其是贮存后期的血液.对于急性失血患者、慢性贫血患者和造血功能障碍患者的输血,应给予保存期较短的血液输注,以提高输血的治疗效果.%Objective To observe the changes in the concentration of hemoglobin, the count of erythrocytes and hematocrit of banked blood conversed in different durations. Methods 264 specimens of banked erythrocytic suspension collected from May 2008 to February 2009 were taken and the concentration of hemoglobin, the count of erythrocytes and hematocrit of the specimens were measured after grouping and conserving in six different durations (0 day, 7 days, 14 days, 21 days, 28 days and 35 days) under the same temperature (4 celsius degree). Results The changes in specimens which were conserved in 7th day, 14th day and 21th day durations were indistinct. Comparing with the former groups, the mentioned indexes in the latter 2 groups evidently showed a decrease or a decline after 28 and 35 days' conservation. Those indexes were certified by t-test, showing that the consequences were statically significant (P < 0.05). Conclusion In the process of conserving blood in vitro, the concentration of hemoglobin, the count of erythrocytes

  18. Thromboxane synthetase inhibitor ameliorates delayed neuronal death in the CA1 subfield of the hippocampus after transient global ischemia in gerbils.

    Science.gov (United States)

    Iijima, T; Sawa, H; Shiokawa, Y; Saito, I; Ishii, H; Nakamura, Z; Sankawa, H

    1996-07-01

    Thromboxane A2 accumulates in the hippocampus after global ischemia and may play a key role in postischemic hypoperfusion. Thromboxane synthetase inhibitor (OKY-046) inhibits the accumulation of thromboxane A2 and promotes prostacycline production. Therefore, we set out to determine whether the inhibition of thromboxane synthesis would ameriolate postischemic neuronal death. Three groups of six Mongolian gerbils were subjected to different treatments: untreated control, untreated ischemia, and treated ischemia. Immediately after forebrain ischemia, OKY-046 (10 mg/kg) was injected intraperitoneally into the treated group. After 7 days of survival, the histopathology of the brain was examined. Pyramidal cell density in the CA1 sector in the treated group was 147 +/- 70 nuclei/mm (mean +/- SD), which was significantly (p < 0.05) higher than than in the untreated group (33 +/- 10 (nuclei/mm). The findings were 231 +/- 7 nuclei/mm for the control group. No significant difference was seen in the profile of temporal muscle temperature before and after ischemia between the groups. Ultrastructurally, the vessels in the CAI sector showed lumen patency in the treated group, whereas occluded vessels with an extended perivascular space were observed in the untreated group. Thromboxane synthetase inhibitor thus partly ameliorates the selective vulnerability of the hippocampus after forebrain ischemia, suggesting that thromboxane A2 is involved in the development of delayed neuronal death, independently of any thermal effect.

  19. 儿童再生障碍性贫血淋巴细胞亚群及与血常规相关性的研究%Lymphocyte subsets and its correlation with blood count in children with aplastic anemia

    Institute of Scientific and Technical Information of China (English)

    曹萍; 蒋莎义; 谢晓恬; 周妮娜; 李福兴; 覃大卫

    2011-01-01

    Objective To investigate the distribution of lymphocyte subsets in peripheral blood and its correlation with complete blood count in children with aplastic anemia. Methods The lymphocyte subsets CD3+ , CD3 + CD4+ , CD3 + CD8 + , CD3+ CD4 VCD3 + CD8 + , CD3 + CD45RA+, CD3 + CD45RO+ , CD(16 +56) + ,CD19 +from 42 aplastic anemia children and 27 controls were examined by flow cytometry; its correlations with complete blood count were analyzed. Results CD3 + CD4+ and CD3 + CD4 VCD3 + CD8+ decreased while CD3 + CD8+ increased in aplastic anemia patients. CD3 + CD8+ was positively correlated with peripheral HB, WBC counts. CD3+ CD4VCD3+ CD8+ was negatively correlated with peripheral HB, WBC and N counts. Conclusion There are abnormal distributions of lymphocyte subsets in aplastic anemia patients, which may be involved in pathogenesisof the disease.%目的 研究再生障碍性贫血患儿外周血淋巴细胞亚群分布,及其与同期外周血血常规的相关性.方法 采用流式细胞技术对我院42例AA患儿和27例正常儿童外周血淋巴细胞亚群CD3+,CD3+ CD4+,CD3+CD8+,CD3+ CD4+/CD3+ CD8+,CD3+ CD45 RA+,CD3+ CD45RO+,CD( 16 +56)+,CD19+的表达进行检测,同时检测血常规.结果 再障组CD3+ CD4+,CD3+ CD8+,CD3+ CD4+/CD3+ CD8+的平均值分别为(26.94±11.93)%,(33.33±10.3)%,0.918±0.53,对照组分别为(33.53±9.97)%,(27.08±9.23)%,1.44±0.80,差异有显著性(P<0.05);CD3+ CD8+与HB,WBC呈一定程度的正相关,CD3+ CD4+/CD3+ CD8+比值与HB,WBC,N呈一定程度的负相关.结论 再障患儿存在淋巴细胞亚群的失调,其中由T淋巴细胞介导的免疫异常对造血功能的抑制在再障的发生中起重要作用,再障患儿Th格局向Th1偏移.

  20. The role of thromboxane A(2) in the pathogenesis of intrauterine growth restriction associated with maternal smoking in pregnancy.

    LENUS (Irish Health Repository)

    Lynch, Caoimhe M

    2012-02-01

    BACKGROUND: To examine the effect of maternal smoking in pregnancy on the production of two eicosanoids, thromboxane A(2) and prostacyclin I2, and their role in the pathogenesis of intrauterine growth restriction. METHODS: Prospective case control study enrolled smoking and non-smoking women at <\\/=14 weeks gestation. Maternal urine samples were obtained at <\\/=14, 28 and 36 weeks. High performance liquid chromatography tandem mass spectrometry (LC-MS-MS) was used to quantify 11-dehydrothromboxane B(2) (TX-M) and 2,3 dinor-6-ketoprostaglandin F1alpha (PG-M), stable urinary metabolites of thromboxane A(2) and prostacyclin I2. Confirmation of the smoking status was performed by quantitation of urinary nicotine metabolites. Data was analysed using SPSS and Stata((R)). RESULTS: Thirty five were enrolled in the smoking group and 32 in the non-smoking group. Smoking resulted higher levels of TX-M at <\\/=14, 28 and 36 weeks gestation. There was no difference in PG-M at any gestational time point between the two groups. The median customised birthweight centile in the smoking group was 17.0 (0-78) compared to 55.5 (4-100) in the non-smoking group (P<0.001). A causal relationship between elevated TX-M and IUGR could not be established. CONCLUSIONS: Maternal smoking in pregnancy is associated with altered eicosanoid production in favour of the vasoconstrictor thromboxane A(2) which occurs early in the first trimester.

  1. Does Dietary Deoxynivalenol Modulate the Acute Phase Reaction in Endotoxaemic Pigs?—Lessons from Clinical Signs, White Blood Cell Counts, and TNF-Alpha

    Directory of Open Access Journals (Sweden)

    Tanja Tesch

    2015-12-01

    Full Text Available We studied the interaction between deoxynivalenol (DON-feeding and a subsequent pre- and post-hepatic immune stimulus with the hypothesis that the liver differently mediates the acute phase reaction (APR in pigs. Barrows (n = 44 were divided into a DON-(4.59 mg DON/kg feed and a control-diet group, surgically equipped with permanent catheters pre- (V. portae hepatis and post-hepatic (V. jugularis interna and infused either with 0.9% NaCl or LPS (7.5 µg/kg BW. Thus, combination of diet (CON vs. DON and infusion (CON vs. LPS, jugular vs. portal created six groups: CON_CONjug.-CONpor., CON_CONjug.-LPSpor., CON_LPSjug.-CONpor., DON_CONjug.-CONpor., DON_CONjug.-LPSpor., DON_LPSjug.-CONpor.. Blood samples were taken at −30, 15, 30, 45, 60, 75, 90, 120, 150, 180 min relative to infusion and analyzed for leukocytes and TNF-alpha. Concurrently, clinical signs were scored and body temperature measured during the same period. LPS as such induced a dramatic rise in TNF-alpha (p < 0.001, hyperthermia (p < 0.01, and severe leukopenia (p < 0.001. In CON-fed pigs, an earlier return to physiological base levels was observed for the clinical complex, starting at 120 min post infusionem (p < 0.05 and persisting until 180 min. DON_LPSjug.-CONpor. resulted in a lower temperature rise (p = 0.08 compared to CON_LPSjug.-CONpor.. In conclusion, APR resulting from a post-hepatic immune stimulus was altered by chronic DON-feeding.

  2. Concentrations of procalcitonin and C-reactive protein, white blood cell count, and the immature-to-total neutrophil ratio in the blood of neonates with nosocomial infections: Gram-negative bacilli vs coagulase-negative staphylococci

    OpenAIRE

    Kordekag, A.

    2011-01-01

    Abstract This study was undertaken to determine whether concentrations of procalcitonin in the blood of neonates with nosocomial infections depend on the type of pathogen. Qualification for the study group was based on the clinical signs of infection. We found that infections with Gram-positive (chiefly coagulase-negative staphylococci) and Gram-negative bacteria are accompanied by elevated concentrations of procalcitonin. In the case of Gram-positive bacteria, other laboratory sig...

  3. Diet Rich in Saturated Fat Decreases the Ratio of Thromboxane/prostacyclin in Healthy Men

    Institute of Scientific and Technical Information of China (English)

    DUO LI; RAYMUNDO HABITO; GEORGE ANGELOS; ANDREW J. SINCLAIR; MADELEINE J. BALL

    2003-01-01

    Objective To investigate the effect of dietary saturated fat (SFA) from animal sources on the urine excretion 11-dehydro thromboxane B2 (TXB2) and 6-keto prostaglandin F 1α (PGF 1α) in 27 healthy free-living male subjects aged 30 to 55 years. Methods It was a randomized crossover design. Each volunteer was randomly assigned to one of the two diets (high fat and low fat) for a period of 4 weeks, after which each subject resumed his usual diet for 2 weeks as a ‘wash-out period',before being assigned to the other diet for an additional 4 weeks. Results Serum proportion of 20:4n-6 was 5% lower in the high fat (6.2% of total fatty acid) than in the low fat diet (6.5% of total fatty acid), which was associated with a significantly decreased ratio of the urinary excretion 11-dehydro TXB2 to 6-keto PGF 1α (P<0.05). However, there was no significant fall in the absolute urinary excretion of 11-dehydro TXB2. Conclusions Diet rich in SFA from animal sources may influence TXA2 formation via effect on tissue proportion of 20:4n-6.

  4. Loss of prostaglandin F2alpha, but not thromboxane, responsiveness in pregnant human myometrium during labour.

    Science.gov (United States)

    Fischer, Deborah P; Hutchinson, Jonathon A; Farrar, Diane; O'Donovan, Peter J; Woodward, David F; Marshall, Kay M

    2008-04-01

    Prostaglandins (PG) E2, PGF2alpha and thromboxane (TX) mediate uterine contractility by targeting prostonoid EP, FP and TP receptors respectively. The aim of this study was to elucidate the function of these receptors in isolated human myometrium taken at term gestation prior to and following labour onset. Lower segment myometrial strips were immersed in organ baths in oxygenated Krebs' solution at 37 degrees C and connected to isometric force transducers. After equilibration, spontaneous activity and concentration responses to PGE2, PGF2alpha and U46619 (a stable TX mimetic) were measured as area under the curve and expressed as a percentage of the final contraction induced by hypotonic shock. Results were expressed as arithmetic means+/-s.e.m. and analysed using two-way ANOVA with Bonferroni's post hoc test. Myometrium excised at late gestation displayed the greatest spontaneous activity compared with the tissues taken during labour (Plabour onset. U46619 consistently stimulated concentration-dependent contractions (Plabour-associated disorders.

  5. Human thromboxane A2 receptor genetic variants: in silico, in vitro and "in platelet" analysis.

    Directory of Open Access Journals (Sweden)

    Scott Gleim

    Full Text Available Thromboxane and its receptor have emerged as key players in modulating vascular thrombotic events. Thus, a dysfunctional hTP genetic variant may protect against (hypoactivity or promote (hyperactivity vascular events, based upon its activity on platelets. After extensive in silico analysis, six hTP-α variants were selected (C(68S, V(80E, E(94V, A(160T, V(176E, and V(217I for detailed biochemical studies based on structural proximity to key regions involved in receptor function and in silico predictions. Variant biochemical profiles ranged from severe instability (C(68S to normal (V(217I, with most variants demonstrating functional alteration in binding, expression or activation (V(80E, E(94V, A(160T, and V(176E. In the absence of patient platelet samples, we developed and validated a novel megakaryocyte based system to evaluate human platelet function in the presence of detected dysfunctional genetic variants. Interestingly, variant V80E exhibited reduced platelet activation whereas A160T demonstrated platelet hyperactivity. This report provides the most comprehensive in silico, in vitro and "in platelet" evaluation of hTP variants to date and highlightscurrent inherent problems in evaluating genetic variants, with possible solutions. The study additionally provides clinical relevance to characterized dysfunctional hTP variants.

  6. Comparison of the effects of ketoprofen and flunixin meglumine on the in vitro response of equine peripheral blood monocytes to bacterial endotoxin.

    Science.gov (United States)

    Jackman, B R; Moore, J N; Barton, M H; Morris, D D

    1994-04-01

    The purpose of this study was to investigate the in vitro effects of flunixin meglumine, a cyclo-oxygenase inhibitor, and ketoprofen, a reported cyclo-oxygenase and lipoxygenase inhibitor, on the synthesis of cyclo-oxygenase end-products thromboxane B2 and prostaglandin E2, lipoxygenase derived 12-hydroxyeicosatetraenoic acid, tumor necrosis factor and tissue factor. Six adult horses were each randomly administered flunixin meglumine (1.1 mg/kg) or ketoprofen (2.2 mg/kg) intravenously every 12 hours with the drug treatments separated by two weeks. Blood samples were obtained prior to initiating treatment, the last day of treatment and for two consecutive days after the termination of treatment for measurement of serum concentrations of thromboxane B2 as well as isolation of peripheral blood monocytes. Quantitation of unstimulated, endotoxin- and calcium ionophore-induced synthesis of thromboxane B2, prostaglandin E2, 12-hydroxyeicosatetraenoic acid, tumor necrosis factor and tissue factor by peripheral blood monocytes was performed in vitro. Both flunixin meglumine and ketoprofen significantly decreased serum concentrations of thromboxane B2 demonstrating in vivo cyclo-oxygenase inhibition. There were no significant differences between drug treatment groups in the in vitro production of thromboxane B2, prostaglandin E2, 12-hydroxy-eicosatetraenoic acid, tumor necrosis factor or tissue factor. This study does not identify significant differences between the effects of flunixin meglumine and ketoprofen.

  7. Correlation analysis of white blood cell differential count with slow coronary flow%白细胞分类计数与冠状动脉慢血流的相关性研究

    Institute of Scientific and Technical Information of China (English)

    张玉鑫; 韩战营

    2015-01-01

    Objective To investigate the correlation of white blood cell differential count with slow coronary flow(SCF).Methods A total of 161 patients confirmed with no significant stenosis through coronary angiography were investigated.Among all the patients,there were 84 patients with SCF and 77 patients with normal coronary flow (NCF).The preoperative fasting venous blood of them was tested to determine the neutrophil count,lymphocyte count,eosinophil count,basophil and monocyte count,then to calculate the neutrophil to lymphocyte ratio(NLR).Results Basal characteristics were similar between the two groups.The NLR,neutrophil and monocyte in SCF group was significantly higher in comparison with the NCF group (2.31 ±0.88、1.96±0.71,P=0.007);(4.14 ± 1.13) ×109、(3.66 ±1.20) ×109,P=0.011; (0.51 ± 0.15) × 109、(0.46 ±0.13) × 109,P =0.017,while eosinophil,basophil and lymphocyte had no difference (1.94±0.63)×109、(1.96±0.55) × 109,p >0.05; (0.17 ±0.12) × 109、(0.15±0.13) ×109,P>0.05; (0.04±0.01) ×109、(0.03 ±0.02) ×109,P >0.05.Logistic regression analysis showed that NLR(OR =3.448,95% CI:1.065-11.165,P =0.039) may be the independent risk factor of SCF phenomenon.ROC curve indicated that NLR (area under curve:0.618,sensitivity:53.6%,specificity:72.7%)had important value on predicting SCF phenomenon.Conclusions Neutrophil and monocyte may be involved in the pathophysiologic process of SCF phenomenon,increased white blood cell count demonstrated SCF phenomenon is a chronic inflammatory state.NLR is the likely independent risk factor of SCF phenomenon,and it can be used as a non-invasive predictor for SCF phenomenon.%目的 探讨白细胞分类计数与冠状动脉慢血流的相关性.方法 对161例经冠状动脉造影检查证实无明显狭窄患者进行研究,其中冠状动脉慢血流(Slow Coronary Flow,SCF)患者84例,正常血流(Normal Coronary Flow,NCF)患者77例.抽取术前空腹静脉血测定中性粒细胞计数、淋巴

  8. Radiological features of pulmonary tuberculosis in human immunodeficiency virus-infected patients: correlation with the blood CD4 cell count; Patrones radiologicos de la tuberculosis pulmonar en pacientes con infeccion VIH: correlacion con el indice de linfocitos CD4 en sangre

    Energy Technology Data Exchange (ETDEWEB)

    Isusi, M.; Eguidazu, J.; Oleaga, L.; Grande, D. [Hospital de Basurto. Bilbao (Spain)

    2000-07-01

    To describe the radiological features of pulmonary tuberculosis (TB) in patients infected with human immunodeficiency virus (HIV) and its correlation with the blood CD4 cell count. We present 44 HIV+patients, 24 with CD4 cell counts of less than 200 cells/mm''3 (group A) and 20 in whom the CD4 counts surpassed this level (group B). We also assessed the chest x-ray images to determine whether or not there was any correlation with the blood CD4 cell counts. Fisher's exact test was used for the statistical study of the differences in the radiological findings in the two groups. The incidence of atypical features was significantly greater in the patients with CD4 cell counts of less than 200 cells/mm''3 (group A) than in those with CD4 counts of over 200 cells/mm''3 (group B). Among HIV+patients, those with a more intact immune status were more likely to present lung x-ray images typical of post-primary TB, with cavitary lesions in upper lobes. The group of patients in whom the immune deficiency was more marked showed a greater incidence of atypical pulmonary findings, more characteristics of primary TB. (Author)

  9. The Big Pumpkin Count.

    Science.gov (United States)

    Coplestone-Loomis, Lenny

    1981-01-01

    Pumpkin seeds are counted after students convert pumpkins to jack-o-lanterns. Among the activities involved, pupils learn to count by 10s, make estimates, and to construct a visual representation of 1,000. (MP)

  10. Relationship between T lymphocytes counts and peripheral blood components in HIV/AIDS patients%HIV/AIDS患者T淋巴细胞计数与外周血组份间关系的研究

    Institute of Scientific and Technical Information of China (English)

    贾海英; 陈莉霞; 马慧霞; 米吉提.买买提

    2012-01-01

    Objective To explore the relationship between peripheral T lymphocyte count and blood test results among HIV/AIDS patients. Methods Anticoagulant were collected from 78 cases of HIV/AIDS (HIV/AIDS group) and 55 cases of healthy volunteers (healthy control group), CD4+T and CD8+T lymphocytes in peripheral blood by flow cytometry were detected , while detected blood cells routine testing were by automatic hematology analyzer.Results 71.79% (56/78) in 78 patients CD4+ T lymphocyte count were <200 cells /μl, 98.72% patients CD47CD8Vere<0.8,among which 75.64% (59/78) patients CD4/CD8 between 0.1~0.5. Those six projects CD4+,CD8+, CD3+,CD4/CD8,WBC and LYMPH both had statistics significance when HIV/ AIDS group compared with control group. While those three projects NEUT,% NEUT and % LYMPH had no statistics significance. Conclusion It is closely related to CD4+,CD8+, CD3+, CD4+CD8+ and LYMPH in the disease process of HIV/ AIDS patients. CD4+,CD8+, blood cells routine testing are detected early to understand the progression of the disease and antiviral therapy time.%目的 分析HIV/AIDS患者外周血T淋巴细胞计数和血常规检测结果.方法 收集78例HIV/AIDS患者(HIV/AIDS组)、55例健康体检人员(健康对照组)的抗凝血,用流式细胞仪技术检测外周血CD4+T及CD8+T淋巴细胞,全自动血细胞分析仪检测血常规.结果 78例患者中有71.79%(56/78) CD4+T淋巴细胞数<200个/μl,98.72%的HIV/AIDS患者CD4+/CD8+比值<0.8,其中75.64% (59/78)患者4/8介于0.1~0.5之间.CD4+、CD8+、CD3+、CD4/CD8、WBC、LYMPH六个项目HIV/AIDS组与健康对照组比较差异均有统计学意义(P<0.05),NEUT、%NEUT及%LYMPH三个项目两组间比较差异无统计学意义(P>0.05).结论 HIV/AIDS患者在发病过程中与CD4+、CD8+、CD3+、CD4+/CD8+及LYMPH密切相关,建议HIV感染者尽早进行医学咨询并做CD4+、CD8+和血常规的检测,以了解病情的进展及进行抗病毒治疗.

  11. Inhibitory Effect of the Punica granatum Fruit Extract on Angiotensin-II Type I Receptor and Thromboxane B2 in Endothelial Cells Induced by Plasma from Preeclamptic Patients.

    Science.gov (United States)

    Kusumawati, Widya; Keman, Kusnarman; Soeharto, Setyawati

    2016-01-01

    This study aims to evaluate whether the Punica granatum fruit extract modulates the Angiotensin-II Type I receptor (AT1-R) and thromboxane B2 level in endothelial cells induced by plasma from preeclamptic patients. Endothelial cells were obtained from human umbilical vascular endothelial cells. At confluence, endothelial cells were divided into five groups, which included endothelial cells exposed to 2% plasma from normal pregnancy (NP), endothelial cells exposed to 2% plasma from preeclamptic patients (PP), and endothelial cells exposed to PP in the presence of ethanolic extract of Punica granatum (PP + PG) at the following three doses: 14; 28; and 56 ppm. The expression of AT1-R was observed by immunohistochemistry technique, and thromboxane B2 level was done by immunoassay technique. Plasma from PP significantly increased AT1-R expression and thromboxane B2 levels compared to cells treated by normal pregnancy plasma. The increasing of AT1-R expression significantly (P Punica granatum extract. Moreover, the increasing of thromboxane B2 levels significantly (P Punica granatum extract. We further concluded that Punica granatum fruit protects and inhibits the sensitivity of endothelial cells to plasma from preeclamptic patients due to inhibition of AT1-R expression (56 ppm) and reduced thromboxane B2 levels (14 ppm).

  12. Clinical evaluation of leukocyte differential count in peripheral blood by five-color flow cytometry%五色流式细胞术分类计数外周血白细胞的临床评价

    Institute of Scientific and Technical Information of China (English)

    邢莹; 王建中; 普程伟; 尚柯

    2013-01-01

    目的 评价五色流式细胞术分类计数外周血白细胞的临床应用价值.方法 选取2010年9至12月北京大学第一医院就诊的265例患者静脉全血标本,以显微镜检查为参考方法,同时用LH750全自动血细胞分析仪和FC500流式细胞仪分类计数白细胞,统计分析显微镜、血细胞分析仪和流式细胞术分类计数外周血白细胞的相关性.选取2010年8至11月北京大学第一医院、北京市道培医院和北京军区总医院的40例患者的静脉全血标本,以显微镜检查为参考方法,同时用LH750全自动血细胞分析仪和NAVIOS流式细胞仪分类计数白细胞,统计分析显微镜、血细胞分析仪和流式细胞术分类计数外周血白细胞的相关性和流式细胞术的临床诊断效率.结果 FC500流式细胞仪采用仪器自动分析软件进行分类,与显微镜分类计数5种白细胞均具有一定相关性(均P<0.01),其中以淋巴细胞、中性粒细胞和嗜酸粒细胞的相关性最佳(r=0.955、0.928、0.876).NAVIOS 流式细胞仪采用手工输入设门策略进行分类,与显微镜方法比较除嗜碱粒细胞外均具有良好的相关性(r >0.700,均P<0.01),其中对检出中性粒细胞、淋巴细胞和原始细胞的相关性较高(r=0.950、0.915、0.852).以显微镜法分类计数外周血不成熟粒细胞1%为临界值,流式细胞术检出不成熟粒细胞的敏感度为87%,特异度为76%.以显微镜法检查外周血原始细胞0.5%为临界值,流式细胞术检出的敏感度为100%,特异度为92%.结论 应用五色流式细胞术在不同流式细胞仪上分类计数外周血白细胞与显微镜法均具有良好的相关性,对检出原始细胞和不成熟粒细胞具有较高的敏感度.%Objective To explore the clinical application values of five-color flow cytometry for leukocyte differential count in peripheral blood.Methods Leukocyte differentiation in 265 peripheral blood samples collected at

  13. Lack of Thromboxane Synthase Prevents Hypertension and Fetal Growth Restriction after High Salt Treatment during Pregnancy.

    Directory of Open Access Journals (Sweden)

    Chen-Hsueh Pai

    Full Text Available Preeclampsia (PE is a potentially fatal pregnancy-related hypertensive disorder characterized by poor placenta development that can cause fetal growth restriction. PE-associated pathologies, including thrombosis, hypertension, and impaired placental development, may result from imbalances between thromboxane A2 (TXA2 and prostacyclin. Low-dose aspirin, which selectively inhibits TXA2 production, is used to prevent high-risk PE. However, the role of TXA2 in aspirin-mediated protective effects in women with PE is not understood fully. In this study, we examined the role of prostanoids in PE using human samples and an induced PE mouse model. We demonstrated that the administration of salted drinking water (2.7% NaCl to wild-type mice resulted in elevated placental TXA2 synthase (TXAS and plasma TXA2, but not prostacyclin, levels, which was also found in our clinical PE placenta samples. The high salt-treated wild-type pregnant mice had shown unchanged maternal body weight, hypertension (MAP increase 15 mmHg, and decreased pup weight (~50% and size (~24%, but these adverse effects were ameliorated in TXAS knockout (KO mice. Moreover, increased expression of interleukin-1β and downstream phosphorylated-p38-mitogen-activated protein kinase were concordant with apoptosis induction in the placentas of salt water-treated wild-type mice. These alterations were not observed in TXAS KO mice. Together, our data suggest that TXA2 depletion has anti-PE effects due to the prevention of hypertension and placental damage through downregulation of the interleukin-1β pathway.

  14. Lack of Thromboxane Synthase Prevents Hypertension and Fetal Growth Restriction after High Salt Treatment during Pregnancy.

    Science.gov (United States)

    Pai, Chen-Hsueh; Yen, Ching-Tzu; Chen, Chie-Pein; Yu, I-Shing; Lin, Shu-Wha; Lin, Shu-Rung

    2016-01-01

    Preeclampsia (PE) is a potentially fatal pregnancy-related hypertensive disorder characterized by poor placenta development that can cause fetal growth restriction. PE-associated pathologies, including thrombosis, hypertension, and impaired placental development, may result from imbalances between thromboxane A2 (TXA2) and prostacyclin. Low-dose aspirin, which selectively inhibits TXA2 production, is used to prevent high-risk PE. However, the role of TXA2 in aspirin-mediated protective effects in women with PE is not understood fully. In this study, we examined the role of prostanoids in PE using human samples and an induced PE mouse model. We demonstrated that the administration of salted drinking water (2.7% NaCl) to wild-type mice resulted in elevated placental TXA2 synthase (TXAS) and plasma TXA2, but not prostacyclin, levels, which was also found in our clinical PE placenta samples. The high salt-treated wild-type pregnant mice had shown unchanged maternal body weight, hypertension (MAP increase 15 mmHg), and decreased pup weight (~50%) and size (~24%), but these adverse effects were ameliorated in TXAS knockout (KO) mice. Moreover, increased expression of interleukin-1β and downstream phosphorylated-p38-mitogen-activated protein kinase were concordant with apoptosis induction in the placentas of salt water-treated wild-type mice. These alterations were not observed in TXAS KO mice. Together, our data suggest that TXA2 depletion has anti-PE effects due to the prevention of hypertension and placental damage through downregulation of the interleukin-1β pathway.

  15. Effects of heparin on platelet aggregation and release and thromboxane A2 production

    Energy Technology Data Exchange (ETDEWEB)

    Mohammad, S.F.; Anderson, W.H.; Smith, J.B.; Chuang, H.Y.; Mason, R.G.

    1981-08-01

    Heparin, when added to citrated platelet-rich plasma (PRP), caused potentiation of platelet aggregation and the release reaction induced by the aggregating agents adenosine diphosphate (ADP), arachidonic acid, collagen, and epinephrine. At low concentrations (4.7 x 10(-5) M) arachidonic acid failed to cause aggregation of platelets in citrated PRP. However, in the presence of heparin, the same concentration of arachidonic acid caused aggregation. Examination of PRP for the presence of thromboxane A2 (TxA2) by use of a bioassay revealed that heparin also stimulated release of TxA2. This finding indicated that platelets released more TxA2 when they were challenged by low concentrations of arachidonic acid in the presence of heparin than in its absence. Platelets were labeled with /sup 3/H-arachidonic acid and /sup 14/C-serotonin, and attempts were made to determine whether heparin stimulated the platelet release reaction first with subsequent increased production of TxA2, or alternatively, whether heparin stimulated TxA2 production first with subsequent enhancement of the release reaction. In view of the demonstrated simultaneous release of /sup 14/C-serotonin and /sup 3/H-arachidonic acid metabolites, it appeared that either release of /sup 14/C and /sup 3/H occurs concurrently or, even if one of these events is dependent on the other, both events take place in rapid succession. Timed sequential studies revealed that in the presence of arachidonic acid, the addition of heparin hastened the apparently simultaneous release of both /sup 14/C and /sup 3/H.

  16. Effect of ozone exposure on lung functions and plasma prostaglandin and thromboxane concentrations in guinea pigs

    Energy Technology Data Exchange (ETDEWEB)

    Miller, P.D.; Ainsworth, D.; Lam, H.F.; Amdur, M.O.

    1987-03-30

    Male Hartley guinea pigs were exposed either to filtered air or to 1 ppm ozone (O/sub 3/) for 1 hr. At 2, 8, 24, or 48 hr after exposure we measured ventilation, respiratory mechanics, lung volumes, diffusing capacity for carbon monoxide (DLCO), and alveolar volume (VA) in anesthetized, tracheotomized animals. Respiratory frequency and tidal volume were unchanged in all groups. Pulmonary resistance was increased 2 hr after O/sub 3/ but returned to control at 8 hr and thereafter. Prolonged reductions in lung volumes (total lung capacity, vital capacity, functional residual capacity, and residual volume) as well as in DLCO and VA occurred after O/sub 3/, with maximum decreases at 8 and 24 hr postexposure. Increased ratios of wet lung weight to body weight were seen at 2, 8, and 24 hr. In separate groups of animals, also exposed either to filtered air or to 1 ppm O/sub 3/, plasma eicosanoid (EC) concentrations were measured at 2, 8, 24, 48, or 72 hr after exposure. Significant increases in thromboxane B2 concentrations were seen at 2, 24, and 48 hr after exposure. Plasma concentrations of 6-keto prostaglandin F1 alpha (PGF1 alpha) and prostaglandin E1 (PGE1) were increased at 24 hr and at 24, 48, and 72 hr, respectively. The nature of this long-term pulmonary response to a short-term exposure to O/sub 3/ suggests alveolar involvement, including probable alveolar duct constriction and localized pulmonary edema. Although changes in plasma EC concentrations were observed concurrent with impaired lung functions, no simple causal relationship was apparent from these studies.

  17. Antiasthmatic activity of a novel thromboxane A2 antagonist, S-1452, in guinea pigs.

    Science.gov (United States)

    Arimura, A; Asanuma, F; Kurosawa, A; Harada, M

    1992-01-01

    We examined the effect of a potent thromboxane (Tx) A2 receptor antagonist, calcium (1R, 2S, 3S, 4S)-(5Z)-7-(((phenylsulfonyl)amino)bicyclo[2.2.1] hept-2-yl)-5-heptenoate dihydrate (S-1452), on antigen- and various allergic-spasmogen-induced contractions of guinea pig lung parenchymal strips and on the increase in insufflation pressure, an index of bronchoconstriction, in anesthetized guinea pigs. In isolated guinea pig lung parenchymal strips, S-1452 showed competitive antagonism of the contractile activity of U-46619, a TxA2 mimetic, with a pA2 value of 8.9. The compound also inhibited the contraction induced by prostaglandin (PG) D2 and PGF2 alpha, but a TxA2 synthetase inhibitor, OKY-046, did not. In contrast, both drugs inhibited not only leukotriene (LT) D4-induced contraction but also antigen-induced contraction in the presence of a histamine antagonist. In anesthetized guinea pigs, oral administration of S-1452 markedly inhibited the bronchoconstrictions induced by intravenous injection of U-46619, PGD2, PGF2 alpha, LTD4 and platelet-activating factor (PAF) with ED50 values of 0.006, 0.031, 0.112, 0.033 and 0.115 mg/kg, respectively, but OKY-046 inhibited only that by LTD4 and PAF. Additionally, bronchoconstriction following intravenous injection of antigen was almost completely suppressed by S-1452 (0.1 mg/kg) and partially by OKY-046 (300 mg/kg) in passively sensitized guinea pigs which were treated with diphenydramine and propranolol. The inhibitory effect of S-1452 against U-46619-induced broncho-constriction persisted up to 7 h after oral administration.(ABSTRACT TRUNCATED AT 250 WORDS)

  18. Examination of thromboxane synthase as a prognostic factor and therapeutic target in non-small cell lung cancer

    LENUS (Irish Health Repository)

    Cathcart, Mary-Clare

    2011-03-09

    Abstract Background Thromboxane synthase (TXS) metabolises prostaglandin H2 into thromboxanes, which are biologically active on cancer cells. TXS over-expression has been reported in a range of cancers, and associated with a poor prognosis. TXS inhibition induces cell death in-vitro, providing a rationale for therapeutic intervention. We aimed to determine the expression profile of TXS in NSCLC and if it is prognostic and\\/or a survival factor in the disease. Methods TXS expression was examined in human NSCLC and matched controls by western analysis and IHC. TXS metabolite (TXB2) levels were measured by EIA. A 204-patient NSCLC TMA was stained for COX-2 and downstream TXS expression. TXS tissue expression was correlated with clinical parameters, including overall survival. Cell proliferation\\/survival and invasion was examined in NSCLC cells following both selective TXS inhibition and stable TXS over-expression. Results TXS was over-expressed in human NSCLC samples, relative to matched normal controls. TXS and TXB2 levels were increased in protein (p < 0.05) and plasma (p < 0.01) NSCLC samples respectively. TXS tissue expression was higher in adenocarcinoma (p < 0.001) and female patients (p < 0.05). No significant correlation with patient survival was observed. Selective TXS inhibition significantly reduced tumour cell growth and increased apoptosis, while TXS over-expression stimulated cell proliferation and invasiveness, and was protective against apoptosis. Conclusion TXS is over-expressed in NSCLC, particularly in the adenocarcinoma subtype. Inhibition of this enzyme inhibits proliferation and induces apoptosis. Targeting thromboxane synthase alone, or in combination with conventional chemotherapy is a potential therapeutic strategy for NSCLC.

  19. Variation in thromboxane B2 concentrations in serum and plasma in patients taking regular aspirin before and after clopidogrel therapy.

    Science.gov (United States)

    Good, Richard I S; McGarrity, Anne; Sheehan, Rory; James, Tina E; Miller, Helen; Stephens, Jonathan; Watkins, Stuart; McConnachie, Alex; Goodall, Alison H; Oldroyd, Keith G

    2015-01-01

    Dual antiplatelet therapy with aspirin and a P2Y12 antagonist is widely prescribed for the prevention of thrombotic events in patients with an acute coronary syndrome or undergoing percutaneous coronary intervention (PCI). It is recognised that there is inter-individual variation in the antiplatelet effects of both drugs. Recent data also suggest that P2Y12 antagonists can affect the response to aspirin. A direct indicator of the effect of aspirin on platelets is their ability to generate thromboxane, which if measured as the difference between the level of thromboxane B2 in serum and plasma ([TxB2]S-P) avoids the confounding effect of endogenous TxB2 production from other cells. We therefore analysed [TxB2]S-P as a measure of aspirin response in a group of 123 patients undergoing elective PCI before and after the introduction of clopidogrel. In a subgroup of 40 patients taking aspirin alone, we compared [TxB2]S-P and VerifyNow Aspirin for the assessment of aspirin response. There was a wide variation in plasma and serum TxB2 concentrations both before and after clopidogrel therapy but only 3.5% of patients had residual serum concentration of TxB2 > 10 ng/ml. There was a strong correlation between the pre and post clopidogrel levels of TxB2 (r ≥ 0.78; p = 0.001) and no significant difference in [TxB2]S-P. There was no correlation between the magnitude of response to clopidogrel response and the generation of thromboxane B2. Correlation between [TxB2]S-P and VerifyNow Aspirin was poor. We conclude that the use of a P2Y12 antagonist does not influence the effect of aspirin on the ability of platelets to generate thromboxane. Therefore, measurement of TxB2 levels in serum, after subtracting the contribution from plasma, provides a measure of the response to aspirin in patients taking dual antiplatelet therapy.

  20. Involvement of COX2–thromboxane pathway in TCDD-induced precardiac edema in developing zebrafish

    Energy Technology Data Exchange (ETDEWEB)

    Teraoka, Hiroki, E-mail: hteraoka@rakuno.ac.jp [School of Veterinary Medicine, Rakuno Gakuen University, Ebetsu (Japan); Okuno, Yuki; Nijoukubo, Daisuke; Yamakoshi, Ayumi [School of Veterinary Medicine, Rakuno Gakuen University, Ebetsu (Japan); Peterson, Richard E. [School of Pharmacy, University of Wisconsin, Madison, WI (United States); Stegeman, John J. [Biology Department, Woods Hole Oceanographic Institution, Woods Hole, MA (United States); Kitazawa, Takio; Hiraga, Takeo [School of Veterinary Medicine, Rakuno Gakuen University, Ebetsu (Japan); Kubota, Akira [School of Veterinary Medicine, Rakuno Gakuen University, Ebetsu (Japan); Biology Department, Woods Hole Oceanographic Institution, Woods Hole, MA (United States)

    2014-09-15

    Highlights: • We establish a new indicator of pericardial edema in developing zebrafish (precardiac edema). • Property of precardiac edema by TCDD is similar to that for conventional pericardial edema. • COX2b (but not COX2a)–thromboxane pathway is involved in precardiac edema by TCDD. - Abstract: The cardiovascular system is one of the most characteristic and important targets for developmental toxicity by 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) in fish larvae. However, knowledge of the mechanism of TCDD-induced edema after heterodimerization of aryl hydrocarbon receptor type 2 (AHR2) and AHR nuclear translocator type 1 (ARNT1) is still limited. In the present study, microscopic analysis with a high-speed camera revealed that TCDD increased the size of a small cavity between the heart and body wall in early eleutheroembryos, a toxic effect that we designate as precardiac edema. A concentration–response curve for precardiac edema at 2 days post fertilization (dpf) showed close similarity to that for conventional pericardial edema at 3 dpf. Precardiac edema caused by TCDD was reduced by morpholino knockdown of AHR2 and ARNT1, as well as by an antioxidant (ascorbic acid). A selective inhibitor of cyclooxygenase type 2 (COX2), NS398, also markedly inhibited TCDD-induced precardiac edema. A thromboxane receptor (TP) antagonist, ICI-192,605 almost abolished TCDD-induced precardiac edema and this effect was canceled by U46619, a TP agonist, which was not influential in the action of TCDD by itself. Knockdown of COX2b and thromboxane A synthase 1 (TBXS), but not COX2a, strongly reduced TCDD-induced precardiac edema. Knockdown of COX2b was without effect on mesencephalic circulation failure caused by TCDD. The edema by TCDD was also inhibited by knockdown of c-mpl, a thrombopoietin receptor necessary for thromobocyte production. Finally, induction of COX2b, but not COX2a, by TCDD was seen in eleutheroembryos at 3 dpf. These results suggest a role of the COX2b–thromboxane

  1. 1,25(OH)2D3 suppresses COX-2 up-regulation and thromboxane production in placental trophoblast cells in response to hypoxic stimulation.

    Science.gov (United States)

    Sun, J; Zhong, W; Gu, Y; Groome, L J; Wang, Y

    2014-02-01

    In this study, we determined if vitamin D could inhibit oxidative stress-induced thromboxane production by placental trophoblasts. Trophoblast isolated from normal placentas were stimulated with CoCl2, a hypoxic mimicking agent, with or without pretreatment of 1,25(OH)2D3. Soluble phospholipase-A2, metabolites of thromboxane-A2 and prostacyclin, and 8-isoprostane were measured. Expression of cyclooxygenase-1 (COX-1), COX-2, and heme oxygenase-1 (HO-1) were determined. We found that pretreatment of trophoblasts with 1,25(OH)2D3 significantly reduced 8-isoprostane and the ratio of thromboxane-A2 to prostacyclin production, and blocked COX-2 expression induced by CoCl2. These results provide evidence of the beneficial effects of vitamin D on placental trophoblasts.

  2. Concentração de anticoagulante, tempo e temperatura de armazenagem sobre os parâmetros hematológicos no hemograma automatizado Anticoagulant concentration, time and storage temperature on hematological parameters in automated blood count

    Directory of Open Access Journals (Sweden)

    Aécio Carlos de Oliveira

    2010-12-01

    concentration. Temperature and time also influenced the MCV, which showed a decrease in time 12 under temperature of 2°C to 8°C and an increase in time 24 and 48 at room temperature. Storage temperature influenced leukocytes and erythrocytes counts, which showed lower values at room temperature. Hemoglobin, PCV, platelet and PCT did not change significantly. The changes observed did not compromise the results obtained by automatic cell counter, showing that blood samples stored for two days remained in good condition for processing, especially when stored under refrigeration.

  3. 脐血造血细胞含量与白血病脐血移植疗效分析%The relationship between the hematopoietic cell counts of cord blood and the therapeutic effect of umbilical cord blood transplantation in patient with leukemia

    Institute of Scientific and Technical Information of China (English)

    许遵鹏; 廖灿; 吴洁莹; 陈劲松; 谢闺娥; 陆琰

    2013-01-01

    Objective To analyze the hematopoietic cell counts in 4711 cord blood unites and explore the relationship between the cell counts and the therapeutic effect of umbilical cord blood transplantation (UCBT). Methods The distribution of total nucleated cells (TNC) and the absolute CD34 + cells counts were analyzed in 4711 cord blood unites. Meanwhile, the differences among hematopoietic cells input, HLA disparity, gender, age, weight, the type of the disease as well as the survival rates were investigated. Results The median counts of TNC and CD34+ cells were 1. 14 × 109 and 4. 06 × 10, respectively, 93.2% of frozen cord blood was suitable for the transplantation in the patients with weight less than 50kg; the engraft rate was 84. 3% (75/89). Neutrophil cells reconstitution at 0. 5 × 109/L, platelet reconstitution at 20 × 109/L and 50 × 109/L occurred at the 17th, 34th and 46th days of UCBT, respectively. Among 75 engrafted cases, 47 patients got complete remission, 26 patients died and 2 patients relapsed. The incidences of acute graft-versus-host disease (GVHD) with grade Ⅰ -Ⅱ, Ⅲ-Ⅳ and chronic GVHD were 54. 7%, 20. 0% and 9. 3%, respectively. The risk factors of engraftment included age, the counts of TNC and CD34 + cells, the overall survival rate was related to the factors including age, weight and the counts of TNC and CD34 +cells. Conclusions Umbilical cord blood could be considered as the source of hematopoietic stem cells and used for the transplantation of the childhood and adult patients with leukemia when there is no acceptably matched bone marrow donor. The cell counts of TNC and CD34 + cells are useful reference index for the selection of cord blood in UCBT.%目的 分析4711份库存脐血造血细胞含量及探讨脐血造血细胞含量与白血病脐血移植疗效的关系.方法 分析4711例库存脐血总有核细胞数(NC)和CD34+细胞数分布情况,探讨不同的造血细胞输入量、供受者HLA不相合数、受者性别、

  4. 北京城区老年人血细胞计数增龄变化及变化范围的调查%Aging changes in routine blood cell count and its reference range in the elderly in Beijing community

    Institute of Scientific and Technical Information of China (English)

    宁尚勇; 李宁华; 韩晓燕; 刘星; 段英伟; 刘玉红; 刘涛; 段晓俐; 郭健

    2014-01-01

    目的 了解北京市中心城区社区65岁以上老年人静脉血常规情况,分析老年人静脉血血常规检测结果与年龄增长之间的关系. 方法 2011年2月至2011年9月,通过随机抽样方法选取北京市朝阳区潘家园社区、八里庄社区和西城区展览路社区、广外社区2014例老年人,通过问卷调查方式收集调查对象的基本资料、生活习惯、患病情况等信息,检测其血常规和生化结果.采用方差分析法分析血常规结果和年龄增长之间的关系. 结果 北京城区老年人的红细胞计数平均值为4.43×1012/L,血红蛋白平均值为135.6 g/L,红细胞压积平均值为39.5%,白细胞平均值为6.69×109/L,血小板平均值为245.8×109/L;调查对象血细胞计数的95%可信区间分别为,红细胞计数(3.50~5.26)×1012/L,血红蛋白106.6~164.6g/L,红细胞压积31.6%~47.5%,白细胞计数(3.26~10.00)×109/L,血小板计数(110.9~377.7)×109/L;男性红细胞计数、血红蛋白和红细胞压积均高于女性(P<0.05、P<0.001和P<0.001),女性血小板计数高于男性(P<0.001);随着年龄增长,红细胞计数、血红蛋白、红细胞压积和平均红细胞血红蛋白浓度(MCHC)逐渐下降(均P<0.001),红细胞平均分布宽度(RDW-CV)和单核细胞数呈递增趋势(均P<0.001). 结论 老年人血细胞计数的参考范围与成年人参考范围有所不同,是否需要单独制定老年人的参考范围还需要更多的数据分析;老年人血细胞计数指标随着年龄的增长呈动态变化,分析其变化原因并提出改善措施可以提高老年人的健康水平.%Objective To investigate routine blood cell count among elderly people aged over 65 years,and to analyze the relationship between routine blood cell count and age.Methods 2014 elderly people were selected by randomly sampling methods from Panjiayuan community and Balizhuang community in Chaoyang District,Zhanlanlu community and Gungwai

  5. Health Physics counting room

    CERN Multimedia

    1970-01-01

    The Health Physics counting room, where the quantity of induced radioactivity in materials is determined. This information is used to evaluate possible radiation hazards from the material investigated.

  6. Timosaponin AIII induces antiplatelet and antithrombotic activity via Gq-mediated signaling by the thromboxane A2 receptor

    Science.gov (United States)

    Cong, Yue; Wang, Limei; Peng, Renjun; Zhao, Yang; Bai, Fan; Yang, Chao; Liu, Xiaolan; Wang, Daqian; Ma, Baiping; Cong, Yuwen

    2016-01-01

    The thromboxane (Tx) A2 pathway is a major contributor to the amplification of initial platelet activation and is therefore a key drug target. To identify potent small-molecule inhibitors of the thromboxane prostaglandin (TP) receptor, we screened a small steroidal saponin library using U46619-induced rat platelet aggregation assays. Timosaponin AIII (TAIII) was identified as a potent inhibitor of U46619-induced rat platelet aggregation and exhibited superior selectivity for the TP receptor versus other G protein-coupled receptors and a PKC activator. TAIII inhibited U46619-induced rat platelet aggregation independent of increases in cAMP and cGMP and the inhibition of TxA2 production. Both PKC and PLC activators restored TAIII-inhibited platelet aggregation, whereas TAIII did not inhibit platelet aggregation induced by co-activation of the G12/13 and Gz pathways. Furthermore, TAIII did not affect the platelet shape change or ROCK2 phosphorylation evoked by low-dose U46619. In vivo, TAIII prolonged tail bleeding time, reduced the mortality of animals with acute pulmonary thromboembolism and significantly reduced venous thrombus weight. Our study suggests that TAIII, by preferentially targeting Gq-mediated PLC/PKC signaling from the TP receptor, induces stronger in vitro antiplatelet activity and in vivo antithrombotic effects and may be an excellent candidate for the treatment of thrombotic disorders. PMID:27934923

  7. Microscopic images dataset for automation of RBCs counting.

    Science.gov (United States)

    Abbas, Sherif

    2015-12-01

    A method for Red Blood Corpuscles (RBCs) counting has been developed using RBCs light microscopic images and Matlab algorithm. The Dataset consists of Red Blood Corpuscles (RBCs) images and there RBCs segmented images. A detailed description using flow chart is given in order to show how to produce RBCs mask. The RBCs mask was used to count the number of RBCs in the blood smear image.

  8. Microscopic images dataset for automation of RBCs counting

    Directory of Open Access Journals (Sweden)

    Sherif Abbas

    2015-12-01

    Full Text Available A method for Red Blood Corpuscles (RBCs counting has been developed using RBCs light microscopic images and Matlab algorithm. The Dataset consists of Red Blood Corpuscles (RBCs images and there RBCs segmented images. A detailed description using flow chart is given in order to show how to produce RBCs mask. The RBCs mask was used to count the number of RBCs in the blood smear image.

  9. Anarthria impairs subvocal counting.

    Science.gov (United States)

    Cubelli, R; Nichelli, P; Pentore, R

    1993-12-01

    We studied subvocal counting in two pure anarthric patients. Analysis showed that they performed definitively worse than normal subjects free to articulate subvocally and their scores were in the lower bounds of the performances of subjects suppressing articulation. These results suggest that subvocal counting is impaired after anarthria.

  10. EcoCount

    Directory of Open Access Journals (Sweden)

    Phillip P. Allen

    2014-05-01

    Full Text Available Techniques that analyze biological remains from sediment sequences for environmental reconstructions are well established and widely used. Yet, identifying, counting, and recording biological evidence such as pollen grains remain a highly skilled, demanding, and time-consuming task. Standard procedure requires the classification and recording of between 300 and 500 pollen grains from each representative sample. Recording the data from a pollen count requires significant effort and focused resources from the palynologist. However, when an adaptation to the recording procedure is utilized, efficiency and time economy improve. We describe EcoCount, which represents a development in environmental data recording procedure. EcoCount is a voice activated fully customizable digital count sheet that allows the investigator to continuously interact with a field of view during the data recording. Continuous viewing allows the palynologist the opportunity to remain engaged with the essential task, identification, for longer, making pollen counting more efficient and economical. EcoCount is a versatile software package that can be used to record a variety of environmental evidence and can be installed onto different computer platforms, making the adoption by users and laboratories simple and inexpensive. The user-friendly format of EcoCount allows any novice to be competent and functional in a very short time.

  11. Hemograma, sódio, potássio, cálcio, osmolalidade e viscosidade durante angiocardiografia pediátrica com ioxaglato Blood cell count, sodium, potassium, calcium, osmolality and viscosity, during pediatric angiocardiography with ioxaglate

    Directory of Open Access Journals (Sweden)

    Mauro Regis Silva Moura

    1998-04-01

    Full Text Available OBJETIVO: Os meios de contraste (MC introduzem alterações em alguns parâmetros sangüíneos, adquirindo, assim, mais importância na angiocardiografia pediátrica. MÉTODOS: Estudamos a presença e a severidade das mudanças no hematócrito, hemoglobina, leucócitos, sódio, potássio, cálcio, osmolalidade e viscosidade, em 35 crianças submetidas a angiocardiografia com ioxaglato, identificando, também, as variáveis independentes responsáveis por essas alterações. As amostras sangüíneas foram colhidas no início do procedimento (S1, no fim (S2 e 2h após (S3. RESULTADOS: Hematócrito: S1= 47,3±6,9%; S2= 40,7±7,4% (pPURPOSE: Children's blood changes during angiocardiography may not be only due to the contrast media (CM. METHODS: We studied the presence and severity of changes in those parameters in 35 pediatric patients undergoing angiocardiography with ioxaglate aiming to identify independent variables responsible for those changes. Blood samples were taken at the beginning of the procedure (S1, at the end (S2 and two hours later (S3. RESULTS: Hematocrit: S1= 47.3±6.9%; S2= 40.7± 7.4% (p<0.001, (related to the CM volume r=0.37, p<0.05. Hemoglobin: S1= 15±2.1g%; S2= 13.2±2.4g% (p<0.001, and S3= 12.7±2.5g% (NS. White blood cell count: S1= 7940±3040 leukocytes/mm³; S2= 6950± 2700/mm³ (NS; S3= 10830±4690 leukocytes/mm³, (p<0.001. Procedure duration (r=0.38, p<0.05 and 5% glucose fluid given between S2 and S3 (r=0.49, p<0.05 were isolated. Sodium: S1= 134.5±0.4mEq/L; S2= 130.7±0.4mEq/L (p<0.001 (due to 5% glucose fluid injected, r=0.61, p<0.01. Potassium: S1= 4.22±0.45mEq/L, S2= 3.83±0.4mEq/L (p<0.001. Calcium: S1= 9.13± 1.03mg%; S2= 8.4±0.91mg/dL. (related to the CM, r=0.43, p<0.01. Osmolality: S1= 293.3±12.5mOsm/kg; S2= 300.6±13.3mOsm/kg (p<0.001. Viscosity: S1= 3.36±0.81; S2= 3.09±0.74 (p<0.01; S3= 3.87±0.89, p<0.001. There was an indirect linear regression with the CM. CONCLUSION: There were profound

  12. Manual and automated reticulocyte counts.

    Science.gov (United States)

    Simionatto, Mackelly; de Paula, Josiane Padilha; Chaves, Michele Ana Flores; Bortoloso, Márcia; Cicchetti, Domenic; Leonart, Maria Suely Soares; do Nascimento, Aguinaldo José

    2010-12-01

    Manual reticulocyte counts were examined under light microscopy, using the property whereby supravital stain precipitates residual ribosomal RNA versus the automated flow methods, with the suggestion that in the latter there is greater precision and an ability to determine both mature and immature reticulocyte fractions. Three hundred and forty-one venous blood samples of patients were analyzed of whom 224 newborn and the rest adults; 51 males and 66 females, with ages between 0 and 89 years, as part of the laboratory routine for hematological examinations at the Clinical Laboratory of the Hospital Universitário do Oeste do Paraná. This work aimed to compare manual and automated methodologies for reticulocyte countings and evaluate random and systematic errors. The results obtained showed that the difference between the two methods was very small, with an estimated 0·4% systematic error and 3·9% random error. Thus, it has been confirmed that both methods, when well conducted, can reflect precisely the reticulocyte counts for adequate clinical use.

  13. Determination of thromboxanes, leukotrienes and lipoxins using high-temperature capillary liquid chromatography-tandem mass spectrometry and on-line sample preparation

    DEFF Research Database (Denmark)

    Dahl, Sandra Rinne; Kleiveland, Charlotte Ramstad; Kassem, Moustapha

    2009-01-01

    An on-line strong cation-exchange (SCX)-reversed-phase (RP) capillary liquid chromatographic (cLC) method with ion-trap tandem mass spectrometric (IT-MS/MS) detection for the simultaneous determination of thromboxane (TX) B(2), TXB(3), leukotriene (LT) B(4), LTD(4) and lipoxin (LX) A(4) in cell...

  14. Dogs with hearth diseases causing turbulent high-velocity blood flow have changes in patelet function and von Willebrand factor multimer distribution

    DEFF Research Database (Denmark)

    Tarnow, Inge; Kristensen, Annemarie Thuri; Olsen, Lisbeth Høier

    2005-01-01

    and echocardiography were performed in all dogs. PFA100 closure times (the ability of platelets to occlude a hole in a membrane at high shear rates), platelet activation markers (plasma thromboxane B2 concentration, platelet surface P-selectin expression), platelet aggregation (in whole blood and platelet-rich plasma...

  15. Single Entity Electrochemistry Progresses to Cell Counting.

    Science.gov (United States)

    Gooding, J Justin

    2016-10-10

    Red blood cells have been counted in an electrochemical collision experiment recently described by Compton and co-workers. As a cell collides with the electrode it lyses and a current is observed from the reduction of oxygen from within the cell.

  16. Effect of a thromboxane A2 receptor antagonist ramatroban (BAY u 3405, on inflammatory cells, chemical mediators and non-specific nasal hyperreactivity after allergen challenge in patients with perennial allergic rhinitis

    Directory of Open Access Journals (Sweden)

    Nobuhisa Terada

    1998-01-01

    Full Text Available In some clinical studies performed in patients with perennial allergic rhinitis, ramatroban, a new thromboxane A2 receptor antagonist, significantly improved nasal symptoms. As yet the mechanism of action of this drug has not been fully elucidated. In the present study we investigated the effects of ramatroban on changes in nasal reactivity and levels of inflammatory cells and mediators in nasal lavage fluid after allergen challenge. Ramatroban was administered orally at a daily dose of 150 mg (b.i.d. for 4 weeks to 11 patients with perennial allergic rhinitis exhibiting positive responses to nasal allergen challenge with house dust mite. Analysis of variance revealed that there was a significant decrease in eosinophil counts and eosinophil cationic protein levels in nasal lavage fluid when compared with values immediately before allergen challenge before and after ramatroban treatment. Histamine, tryptase and albumin levels were significantly decreased in analysis of variance before and after ramatroban treatment. The degree of nasal reactivity to histamine was also significantly decreased after the ramatroban treatment. These findings indicate that ramatroban decreases important pathogenic factors in allergic rhinitis, resulting in an improvement in nasal symptoms.

  17. Sublattice Counting and Orbifolds

    CERN Document Server

    Hanany, Amihay; Reffert, Susanne

    2010-01-01

    Abelian orbifolds of C^3 are known to be encoded by hexagonal brane tilings. To date it is not known how to count all such orbifolds. We fill this gap by employing number theoretic techniques from crystallography, and by making use of Polya's Enumeration Theorem. The results turn out to be beautifully encoded in terms of partition functions and Dirichlet Series. The same methods apply to counting orbifolds of any toric non-compact Calabi-Yau singularity. As additional examples, we count the orbifolds of the conifold, of the L^{aba} theories, and of C^4.

  18. 血小板聚集影响因素在血细胞计数中的临床应用%Clinical application of platelet aggregation for white blood cell count

    Institute of Scientific and Technical Information of China (English)

    胡恩亮; 赵媛; 王妍; 樊爱琳; 郑善銮

    2016-01-01

    Objective To analyze the cause of platelet aggregation in blood specimens ,so as to provide basis for reducing platelet aggregation ,and avoiding false positive of platelet count ,false report ,misdiagnosis and mistreatment .Methods The blood speci-mens which platelet was below 80 × 109 /L ,below 125 × 109 /L with histogram hinted platelet aggregation ,were smeared ,stained with Wright-Giemsa ,and observed by microscope for platelet morphological changes .The data between each groups were calculated and analyzed by statistical software SPSS version 18 .0 .Results A total of 184 cases of ethylenediaminetetraacetic acid dependent pseudothrombocytopenia(EDTA-PTCP) were found ,accounted for 0 .444 ‰ totally ,including 0 .244 ‰ of out-patients (101 cases) , 0 .159 ‰ of hospitalized patients (66 cases) ,and 0 .041 ‰ of health examination personnel (17 cases) .3 cases of multi-dependent pseudothrombocytopenia and 25 cases of pseudo platelet aggregation were found ,and accounted for 0 .007 ‰ and 0 .060 ‰ respec-tively .Conclusion The discovery of platelet aggregation which caused mainly by EDTA-PTCP ,still relies on microscopy ,and pseu-do platelet aggregation comes mainly from sampling ,so it needs to strengthen the skills training .%目的:分析血小板聚集影响因素,为降低血小板聚集所致血小板假性减低、实验室规避报告风险及减低误诊误治提供依据。方法对血小板小于80×109/L 、小于125×109/L 合并直方图提示血小板凝集标本进行推片、瑞氏-吉姆萨染色后显微镜下观察是否聚集,并采用统计软件 SPSS 18.0进行统计分析。结果乙二胺四乙酸依赖性血小板减少症(EDTA-PTCP)共计184例,占0.444‰,其中门诊患者101例,占0.244‰,住院患者66例,占0.159‰,体检者17例,占0.041‰;多重抗凝剂依赖性血小板假性减少共计3例,占0.007‰,假性血小板聚集共计25例,占0.060‰。结论血小

  19. Allegheny County Traffic Counts

    Data.gov (United States)

    Allegheny County / City of Pittsburgh / Western PA Regional Data Center — Traffic sensors at over 1,200 locations in Allegheny County collect vehicle counts for the Pennsylvania Department of Transportation. Data included in the Health...

  20. Counting Belief Propagation

    CERN Document Server

    Kersting, Kristian; Natarajan, Sriraam

    2012-01-01

    A major benefit of graphical models is that most knowledge is captured in the model structure. Many models, however, produce inference problems with a lot of symmetries not reflected in the graphical structure and hence not exploitable by efficient inference techniques such as belief propagation (BP). In this paper, we present a new and simple BP algorithm, called counting BP, that exploits such additional symmetries. Starting from a given factor graph, counting BP first constructs a compressed factor graph of clusternodes and clusterfactors, corresponding to sets of nodes and factors that are indistinguishable given the evidence. Then it runs a modified BP algorithm on the compressed graph that is equivalent to running BP on the original factor graph. Our experiments show that counting BP is applicable to a variety of important AI tasks such as (dynamic) relational models and boolean model counting, and that significant efficiency gains are obtainable, often by orders of magnitude.

  1. Analog multivariate counting analyzers

    CERN Document Server

    Nikitin, A V; Armstrong, T P

    2003-01-01

    Characterizing rates of occurrence of various features of a signal is of great importance in numerous types of physical measurements. Such signal features can be defined as certain discrete coincidence events, e.g. crossings of a signal with a given threshold, or occurrence of extrema of a certain amplitude. We describe measuring rates of such events by means of analog multivariate counting analyzers. Given a continuous scalar or multicomponent (vector) input signal, an analog counting analyzer outputs a continuous signal with the instantaneous magnitude equal to the rate of occurrence of certain coincidence events. The analog nature of the proposed analyzers allows us to reformulate many problems of the traditional counting measurements, and cast them in a form which is readily addressed by methods of differential calculus rather than by algebraic or logical means of digital signal processing. Analog counting analyzers can be easily implemented in discrete or integrated electronic circuits, do not suffer fro...

  2. 1996 : Track Count Protocol

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — The goal of St. Vincent National Wildlife Refuge's Track Count Protocol is to provide an index to the population size of game animals inhabiting St. Vincent Island.

  3. Housing Inventory Count

    Data.gov (United States)

    Department of Housing and Urban Development — This report displays the data communities reported to HUD about the nature of their dedicated homeless inventory, referred to as their Housing Inventory Count (HIC)....

  4. The association of thromboxane A2 receptor with lipid rafts is a determinant for platelet functional responses.

    Science.gov (United States)

    Moscardó, A; Vallés, J; Latorre, A; Santos, M T

    2014-08-25

    We have investigated the presence of thromboxane A2 (TXA2) receptor associated with lipid rafts in human platelets and the regulation of platelet function in response to TXA2 receptor agonists when lipid rafts are disrupted by cholesterol extraction. Platelet aggregation with TXA2 analogs U46619 and IBOP was almost blunted in cholesterol-depleted platelets, as well as αIIbβ3 integrin activation and P-selectin exposure. Raft disruption also inhibited TXA2-induced cytosolic calcium increase and nucleotide release, ruling out an implication of P2Y12 receptor. An important proportion of TXA2 receptor (40%) was colocalized at lipid rafts. The presence of the TXA2 receptor associated with lipid rafts in platelets is important for functional platelet responses to TXA2.

  5. Counting RG flows

    OpenAIRE

    Gukov, Sergei

    2016-01-01

    Interpreting renormalization group flows as solitons interpolating between different fixed points, we ask various questions that are normally asked in soliton physics but not in renormalization theory. Can one count RG flows? Are there different "topological sectors" for RG flows? What is the moduli space of an RG flow, and how does it compare to familiar moduli spaces of (supersymmetric) dowain walls? Analyzing these questions in a wide variety of contexts --- from counting RG walls to AdS/C...

  6. Counting curves on surfaces

    OpenAIRE

    2015-01-01

    In this paper we consider an elementary, and largely unexplored, combinatorial problem in low-dimensional topology. Consider a real 2-dimensional compact surface $S$, and fix a number of points $F$ on its boundary. We ask: how many configurations of disjoint arcs are there on $S$ whose boundary is $F$? We find that this enumerative problem, counting curves on surfaces, has a rich structure. For instance, we show that the curve counts obey an effective recursion, in the general framework of to...

  7. Neutron counting with cameras

    Energy Technology Data Exchange (ETDEWEB)

    Van Esch, Patrick; Crisanti, Marta; Mutti, Paolo [Institut Laue Langevin, Grenoble (France)

    2015-07-01

    A research project is presented in which we aim at counting individual neutrons with CCD-like cameras. We explore theoretically a technique that allows us to use imaging detectors as counting detectors at lower counting rates, and transits smoothly to continuous imaging at higher counting rates. As such, the hope is to combine the good background rejection properties of standard neutron counting detectors with the absence of dead time of integrating neutron imaging cameras as well as their very good spatial resolution. Compared to Xray detection, the essence of thermal neutron detection is the nuclear conversion reaction. The released energies involved are of the order of a few MeV, while X-ray detection releases energies of the order of the photon energy, which is in the 10 KeV range. Thanks to advances in camera technology which have resulted in increased quantum efficiency, lower noise, as well as increased frame rate up to 100 fps for CMOS-type cameras, this more than 100-fold higher available detection energy implies that the individual neutron detection light signal can be significantly above the noise level, as such allowing for discrimination and individual counting, which is hard to achieve with X-rays. The time scale of CMOS-type cameras doesn't allow one to consider time-of-flight measurements, but kinetic experiments in the 10 ms range are possible. The theory is next confronted to the first experimental results. (authors)

  8. Identification and characterization of a putative human platelet thromboxane A/sub 2//prostaglandin H/sub 2/ receptor

    Energy Technology Data Exchange (ETDEWEB)

    Saussy, D.L. Jr.

    1986-01-01

    The thromboxane A/sub 2/ (TXA/sub 2/) analog, 9,11-dimethylmethano-11,12-methano-16-(3-iodo-4-hydroxyphenyl)-13,14-dihydro-13-aza-15..cap alpha beta..-omega-tetranor TXA/sub 2/ (I-PTA-OH) was characterized as a competitive antagonist of TXA/sub 2/ mimetic-induced platelet aggregation, with a K/sub d/ of 190 nM in platelet rich plasma. This antagonism was specific for the putative thromboxane A/sub 2//prostaglandin H/sub 2/ (TXA/sub 2//PGH/sub 2/) receptor, since I-PTA-OH had no inhibitory effects on platelet aggregation stimulated by agonists which act independently of TXA/sub 2//PGH/sub 2/, and did not inhibit platelet TXA/sub 2/ synthesis. (/sup 125/I)-PTA-OH binding to a particulate fraction from human platelets was saturable, displaceable, and linear with protein concentration. Scatchard analysis of equilibrium binding revealed a single class of high affinity binding sites, with a K/sub d/ of 30 +/- 4 nM and a B/sub max/ of 1.8 +/- 0.3 pmol/mg protein. Kinetic analysis yielded a k/sub 1/ of 1.35 x 10/sup 6/ M/sup -1/ x min/sup -1/ and a k..sqrt../sub 1/ of 0.032 min/sup -1/, K/sub d/ = k..sqrt../sub 1//k/sub 1/ = 24 nM. The subcellular localization of the putative TXA/sub 2//PGH/sub 2/ receptor was determined using (/sup 125/I)-PTA-OH binding as a marker for the receptor. (/sup 125/I)-PTA-OH binding as a marker for the receptor. (/sup 125/I)-PTA-OH binding, was coenriched with markers for plasma membranes and dense tubular system; but not with markers for cytoplasmic constituents, mitochondria, or granules.

  9. Total lymphocyte count and subpopulation lymphocyte counts in relation to dietary intake and nutritional status of peritoneal dialysis patients.

    Science.gov (United States)

    Grzegorzewska, Alicja E; Leander, Magdalena

    2005-01-01

    Dietary deficiency causes abnormalities in circulating lymphocyte counts. For the present paper, we evaluated correlations between total and subpopulation lymphocyte counts (TLC, SLCs) and parameters of nutrition in peritoneal dialysis (PD) patients. Studies were carried out in 55 patients treated with PD for 22.2 +/- 11.4 months. Parameters of nutritional status included total body mass, lean body mass (LBM), body mass index (BMI), and laboratory indices [total protein, albumin, iron, ferritin, and total iron binding capacity (TIBC)]. The SLCs were evaluated using flow cytometry. Positive correlations were seen between TLC and dietary intake of niacin; TLC and CD8 and CD16+56 counts and energy delivered from protein; CD4 count and beta-carotene and monounsaturated fatty acids 17:1 intake; and CD19 count and potassium, copper, vitamin A, and beta-carotene intake. Anorexia negatively influenced CD19 count. Serum albumin showed correlations with CD4 and CD19 counts, and LBM with CD19 count. A higher CD19 count was connected with a higher red blood cell count, hemoglobin, and hematocrit. Correlations were observed between TIBC and TLC and CD3 and CD8 counts, and between serum Fe and TLC and CD3 and CD4 counts. Patients with a higher CD19 count showed a better clinical-laboratory score, especially less weakness. Patients with a higher CD4 count had less expressed insomnia. Quantities of ingested vitamins and minerals influence lymphocyte counts in the peripheral blood of PD patients. Evaluation of TLC and SLCs is helpful in monitoring the effectiveness of nutrition in these patients.

  10. Survey of comparability and accuracy of complete blood count among third level hospitals in Beijing%北京市三级医院全血细胞计数结果可比性和准确性调查

    Institute of Scientific and Technical Information of China (English)

    彭明婷; 岳育红; 申子瑜; 王清涛; 陆红; 李臣宾; 谷小林; 陈文祥

    2007-01-01

    目的 对北京市三级医院间全血细胞计数结果的可比性和准确性进行调查,为开展临床检验结果的互认提供依据.方法 共调查41家实验室,以全血细胞计数(complete blood count,CBC)为试点项目,通过现场调查、仪器校准和结果比对等方式,发现存在的问题,制定相应的管理措施、纠正措施和评价方法.结果 在仪器校准和室内质量控制方面存在较多有待规范之处,表现为22家实验室(53.6%)无校准记录,甚至有4家实验室(9.8%)从未进行过校准,4家实验室(9.8%)使用质控物而非校准物进行了错误的校准;14家实验室(34.2%)开展室内质量控制项目的数量达不到要求.此外,有4家实验室(9.8%)使用非配套试剂.根据调查数据和检验结果生物变异的文献报道,将北京市血细胞计数结果的允许偏差暂定为:WBC≤15%,Hb≤5%,RBC≤5%,红细胞压积(HCT)≤5%,PLT≤15%.通过使用卫生部临床检验中心血细胞分析校准实验室提供的溯源至参考方法的定值新鲜血对血液分析仪进行校准,使13家医院的14个不合格项目纠正了11个.结论 通过现场调查、使用具有溯源性的定值新鲜血校准不同的检测系统、现场的结果比对、质量管理要求的提出以及监督措施的实施,可以明显提高医院间检验结果的可比性和准确性,为开展检验结果的互认提供了依据.

  11. Análisis de productividad, calidad y costos en laboratorios del primer nivel: la biometría hemática Analysis of productivity, quality and cost of first grade laboratories: complete blood count

    Directory of Open Access Journals (Sweden)

    Leticia Avila

    1999-04-01

    tiveram resultados confiáveis. Somente 20% dos laboratórios trabalharam com eficiência. CONCLUSÕES: Para solucionar os problemas encontrados será preciso usar estratégias integrais que comprometam os diferentes setores, mas de solução difícil. Recomenda-se analisar o custo-benefício de criar um laboratório central e deixar os demais laboratórios com a função exclusiva de selecionar amostras.OBJECTIVE: Assessment of productivity, quality and production costs and determination of the efficiency of top grade clinical laboratories in Mexico. METHODS: Ten laboratories were selected from among the total number (52 existing in Mexico City, and the Donabedian model of structure, process and results were applied. Blood count was selected as a tracer. RESULTS: The principal problems found were: inadequate distribution of trained human resources, poor glass material, inadequate analytic process and low productivity. These factors are reflected in the unit costs, which exceed reference laboratory costs by 200%. Only 50% of the laboratories analyzed generate reliable results. Only 20% of the laboratories studied operate efficiently. CONCLUSIONS: To solve the problems identified requires integral srategies at different levels. A specific recomendation for the improvement of quality and productivity is an assessment of the cost/benefit of creating a central laboratory and using the remaining sites exclusively for the collection of samples.

  12. Photon counting digital holography

    Science.gov (United States)

    Demoli, Nazif; Skenderović, Hrvoje; Stipčević, Mario; Pavičić, Mladen

    2016-05-01

    Digital holography uses electronic sensors for hologram recording and numerical method for hologram reconstruction enabling thus the development of advanced holography applications. However, in some cases, the useful information is concealed in a very wide dynamic range of illumination intensities and successful recording requires an appropriate dynamic range of the sensor. An effective solution to this problem is the use of a photon-counting detector. Such detectors possess counting rates of the order of tens to hundreds of millions counts per second, but conditions of recording holograms have to be investigated in greater detail. Here, we summarize our main findings on this problem. First, conditions for optimum recording of digital holograms for detecting a signal significantly below detector's noise are analyzed in terms of the most important holographic measures. Second, for time-averaged digital holograms, optimum recordings were investigated for exposures shorter than the vibration cycle. In both cases, these conditions are studied by simulations and experiments.

  13. Urine white blood cell counts and level of serum C-reactive protein for assessment of risk of infections after lithotrity%尿白细胞计数与血 C-反应蛋白水平对碎石术后感染风险的评估

    Institute of Scientific and Technical Information of China (English)

    肖友平; 黄从军; 范凯; 王波; 李鹏程

    2015-01-01

    OBJECTIVE To explore the urine white blood cell counts and level of serum C-reactive protein (CRP) for assessment of infections after the transurethral lithotripsy under ureteroscope so as to reduce the incidence of the postoperative infections .METHODS A total of 720 hospitalized patients who underwent the transurethral litho -tripsy under ureteroscope were enrolled in the study ,the urine white blood cell counts and the level of serum CRP were determined within 24 hours before the surgery ,then the 10/HP of urine white blood cell counts and 8 mg/L of serum CRP were defined as the boundary ,the incidence of the postoperative infections was compared .RESULTS Of the 720 patients ,174 were tested positive for the white blood cell counts before the surgery ,and 201 were test-ed positive for the serum CRP .Totally 99 cases had the postoperative infections ,the incidence of the postoperative infections was 28 .86% in the patients tested positive for CRP before the surgery ,significantly higher than 5 .97%in the patients tested negative for CRP (χ2 = 53 .651 ,P< 0 .001) .The patients with postoperative infections tested positive for both the urine white blood cell counts and serum CRP accounted for 35 .71% ,as compared with 26 .21% of the patients tested negative for the white blood cell counts and positive for serum CRP ,there was no statistically significant difference .The incidence of the postoperative infections was significantly higher in the pa-tients tested positive for both urine white blood cell counts and CRP than in the patients tested positive for urine white blood cell counts and negative for serum CRP and than the patients tested negative for both urine white blood cell counts and serum CRP (P< 0 .01) .CONCLUSION The level of preoperative CRP more than 8 mg/L is the ma-jor risk factor for the postoperative infection and can be used as an indicator for the conduct of the transurethral lithotripsy under ureteroscope ,as compared with the urine white blood

  14. The significances of high sensitivity C-reactive protein, white blood cell count, and neutral granular cell ratio in children with infection%儿童hsCRP、白细胞计数和中性粒细胞比例在感染中的意义

    Institute of Scientific and Technical Information of China (English)

    张文娟; 曹肖琲; 金美玉; 李善玉

    2013-01-01

    目的:通过分析呼吸道感染患儿的超敏C-反应蛋白(hsCRP)、白细胞计数(WBC)及性粒细胞比值(N%)的检测结果,探讨hsCRP、WBC及N%在患儿感染中的临床意义和相关性.方法:回顾性分析1 196例该院2011年收治的呼吸道感染患儿,根据患儿感染的病原体分为细菌组、病毒组及支原体组,同时选择200例该院健康体检儿童为对照组,检测并比较各组hsCRP、WBC、hsCRP、N%.结果:统计分析显示,细菌组患儿的hsCRP、WBC及N%均显著升高,且多数情况下为hsCRP、WBC、N%同时升高或hsCRP升高伴WBC或N%升高,尤其以N%与hsCRP的相关性更明显;病毒组患几的N%显著下降,hsCRP及WBC均正常;支原体感染患儿的hsCRP、WBC及N%升高不明显,多数为hsCRP、WBC、N%中一至两项轻度升高;在分层研究中,细菌组和支原体感染组N%和hsCRP呈正相关,高于WBC和hsCRP的相关性.结论:在呼吸道感染患儿中,联合检测hsCRP、WB、N%可有效鉴别细菌、病毒及支原体感染,进而指导临床治疗,尤其hsCRP与N%同时明显升高提示细菌感染,应根据病情及早选用有效的抗生素进行治疗.%Objective: To explore the clinical significances of high sensitivity C -reactive protein, white blood cell count, and neutral granular cell ratio in children with infection and the correlation by analyzing the detection results. Methods: A total of 1 196 children with respiratory tract infection who were treated in the hospital in 2011 were analyzed retrospectively, then the children were divided into bacteria group, virus group, and mycoplasma group according to different pathogens; 200 healthy children who received health physical examination in the hospital were selected as control group; the high sensitivity C - reactive protein levels, white blood cell counts, and neutral granular cell ratios in different groups were detected. Results: Statistical analysis showed that in bacterial group

  15. Hematopoietic progenitor cell counting for prediction of the yield of peripheral blood stem cell in mobilization and harvest%外周血造血祖细胞检测对外周血干细胞动员采集的预测意义

    Institute of Scientific and Technical Information of China (English)

    魏征; 王志梅; 庄静丽; 李锋; 程韵枫; 邹善华

    2013-01-01

    目的 通过血细胞分析仪检测外周血造血祖细胞(HPC)含量,探寻一种快速预测采集物干细胞含量、判断外周血造血干细胞采集时机的手段,并评价其应用意义.方法 对27例化疗联合粒细胞集落刺激因子(G-CSF)进行外周血造血干细胞动员采集者及17例单用G-CSF动员的采集者,利用血细胞分析仪检测外周血HPC计数,血液治疗和移植工程国际组织(ISHAGE)法检测外周血及采集物CD34+细胞计数,并进行相关回归分析.对外周血HPC计数预测采集物CD34+含量进行受试者工作曲线(ROC)分析.结果 无论化疗组或非化疗组,外周血HPC计数与CD34+计数均具有线性相关性(r=0.711,P=0.000及r=0.656,P=0.004).化疗组外周血CD34+计数=-0.829+0.648×外周血HPC计数;非化疗组外周血CD34+细胞计数=45.033+0.460×外周血HPC计数.化疗组患者外周血HPC计数与采集物CD34+细胞量呈线性相关(r=0.602,P=0.001),采集物CD34+计数=1.106+0.046×外周血HPC计数.以HPC≥85/μl预测采集物CD34+计数≥5×106/kg的灵敏度为78%,特异度为82%.结论 在自体干细胞移植动员患者中,外周血HPC计数可部分替代外周血CD34+计数预测采集效果,HPC≥85/μl为较好的预测阈值.%Objective To evaluate a method of fast detection of the hematopoietic progenitor cell (HPC) in peripheral blood samples and explore for an appropriate cutoff value in prediction of adequate CD34+ cell in apheresis concentrate.Methods Peripheral blood samples and apheresis concentrate samples were collected from 27 auto-PBSCT patients receiving chemotherapy plus G-CSF mobilization (chemo group) and 17 patients receiving G-CSF alone (non-chemo group).CD34+ cell counts were determined by flow cytometry according to ISHAGE guideline and HPC counts were detected using Sysmex XE-2100 automatic hemocyte analyzer.The correlation between HPC and CD34+ cell counts in peripheral blood samples and apheresis concentrates were analyzed

  16. Subarachnoid hemorrhage induces enhanced expression of thromboxane A2 receptors in rat cerebral arteries

    DEFF Research Database (Denmark)

    Ansar, Saema; Larsen, Carl; Maddahi, Aida;

    2010-01-01

    after SAH in cerebral arteries. SAH was induced in rats by injecting 250 microl of blood into the prechiasmatic cistern. Two days after the SAH, cerebral arteries were harvested and contractile responses to the TP receptor agonist U46619 were investigated with myographs. In addition, the contractile...

  17. What Counts as Prostitution?

    Directory of Open Access Journals (Sweden)

    Stuart P. Green

    2016-08-01

    Full Text Available What counts, or should count, as prostitution? In the criminal law today, prostitution is understood to involve the provision of sexual services in exchange for money or other benefits. But what exactly is a ‘sexual service’? And what exactly is the nature of the required ‘exchange’? The key to answering these questions is to recognize that how we choose to define prostitution will inevitably depend on why we believe one or more aspects of prostitution are wrong or harmful, or should be criminalized or otherwise deterred, in the first place. These judgements, in turn, will often depend on an assessment of the contested empirical evidence on which they rest. This article describes a variety of real-world contexts in which the ‘what counts as prostitution’ question has arisen, surveys a range of leading rationales for deterring prostitution, and demonstrates how the answer to the definition question depends on the answer to the normative question. The article concludes with some preliminary thoughts on how analogous questions about what should count as sexual conduct arise in the context of consensual offences such as adultery and incest, as well as non-consensual offences such as sexual assault.

  18. Reticulocyte Count Test

    Science.gov (United States)

    ... may be ordered when: CBC results show a decreased RBC count and/or a decreased hemoglobin and hematocrit A healthcare practitioner wants to ... and hematocrit, to help determine the degree and rate of overproduction of RBCs ... during pregnancy . Newborns have a higher percentage of reticulocytes, but ...

  19. What Counts as Evidence?

    Science.gov (United States)

    Dougherty Stahl, Katherine A.

    2014-01-01

    Each disciplinary community has its own criteria for determining what counts as evidence of knowledge in their academic field. The criteria influence the ways that a community's knowledge is created, communicated, and evaluated. Situating reading, writing, and language instruction within the content areas enables teachers to explicitly…

  20. Acetaminophen and Meloxicam Inhibit Platelet Aggregation and Coagulation in Blood Samples from Humans

    Science.gov (United States)

    2014-01-01

    participant was sampled once with a total of 100-ml blood volume. Exclusion criteria included pregnancy, on- going therapeutic anticoagulation , and use...of thromboxane A2 (TxA2) from prostaglandin H2, which is generated from arachidonic acid by cyclo-oxygenase (COX-1). The antiplatelet effects of...is acetaminophen? Some practical cautions with this widely used agent . Clin Pediatr (Phila) 1973; 12:692– 696. 3 Whyte IM, Buckley NA, Reith DM

  1. The Thromboxane Receptor Antagonist S18886 influence the stability of atherosclerosis in ApoE Null Mice

    Institute of Scientific and Technical Information of China (English)

    Cong Hongliang; Xiang Nina; Chen Yingchong; Julie H

    2005-01-01

    Objectives To investigate whether thromboxane receptor antagonist S18886 inhibits infiltrating macrophages to vessel wall and influence the morphology of atherosclerotic plaque;The effective of S18886 compared to clopidegrol on the development of atherosclerosis, accumulation of lipidfilled macropha-ges in apoE null mice. Methods All mice were done cuffed common carotid artery and fed a Western-type atherogenic diet for 6 weeks from the day of surgery, at same time the therapy group mice were gavaged S18886 5 mg/Kg/day and clopidegrol respectively, the same volume water were gavaged as the placebo group. Results profound inhibition of lesion area growth after cuff of the right common carotid artery in mice with 5 mg/kg of S18886, markdely reduce intima to media ratio and intima to total wall area compare with clopidegrol or blank group; Macrophage infiltration into sites of arterial plaque was also markedly attenuated by ICAM-1 deficiency in the S18886 group, whereas inside the arterial wall plaque of placebo apoE null mice α-smooth muscle actin markedly attenuated. Treatment with 25 mg/kg/day clopidegrol reduced the level of ICAM-1 staining, both S18886 and clopidegrol didn't influence the α-smooth muscle actin inside plaque. Conclusions It was considered that the novel anti-thrombotic drug significant reduce macrophage infiltration in the sites of arterial plaque by ICAM- 1 deficiency, S 18886 not only reduce the size, but also stabilized the plaque.

  2. Increased cyclooxygenase-2 and thromboxane synthase expression is implicated in diosgenin-induced megakaryocytic differentiation in human erythroleukemia cells.

    Science.gov (United States)

    Cailleteau, C; Liagre, B; Battu, S; Jayat-Vignoles, C; Beneytout, J L

    2008-09-01

    Differentiation induction as a therapeutic strategy has, so far, the greatest impact in hematopoietic malignancies, most notably leukemia. Diosgenin is a very interesting natural product because, depending on the specific dose used, its biological effect is very different in HEL (human erythroleukemia) cells. For example, at 10 microM, diosgenin induced megakaryocytic differentiation, in contrast to 40 microM diosgenin, which induced apoptosis in HEL cells previously demonstrated using sedimentation field-flow fractionation (SdFFF). The goal of this work focused on the correlation between cyclooxygenase-2 (COX-2) and thromboxane synthase (TxS) and megakaryocytic differentiation induced by diosgenin in HEL cells. Furthermore, the technique of SdFFF, having been validated in our models, was used in this new study as an analytical tool that provided us with more or less enriched differentiated cell fractions that could then be used for further analyses of enzyme protein expression and activity for the first time. In our study, we showed the implication of COX-2 and TxS in diosgenin-induced megakaryocytic differentiation in HEL cells. Furthermore, we showed that the analytical technique of SdFFF may be used as a tool to confirm our results as a function of the degree of cell differentiation.

  3. Prostaglandin cyclooxygenase products but not thromboxane A2 are involved in the pathogenesis of ewthromelalgia in thrombocythaemia

    Directory of Open Access Journals (Sweden)

    J. J. Michiels

    1993-01-01

    Full Text Available Fluid of artificial blisters from erythromelalgic skin areas in primary thrombocythaemia contained a high amount of prostaglandin-E-like activity. Dazoxiben did not alleviate the erythromelalgia in patients with primary thrombocythaemia despite complete inhibition of platelet malondialdehyde and thromboxane B2 synthesis and no inhibition of prostaglandin-E-like material. During a 10-day dazoxiben treatment period, persistent erythromelalgia was associated with a significant shortened mean platelet life span of 3.2 days. During subsequent treatment with low dose acetylsalicylic acid daily complete relief of erythromelalgia was associated with inhibition of platelet prostaglandin endoperoxide production and correction of platelet mean life span to normal, 7.9 days. These observations indicate that prostaglandin E2, or another prostaglandin endoperoxide metabolite, is involved in the pathogenesisof erythromelalgia. The presented study does not give one single clue as to the origin (platelet, vessel wall or other of the prostanoid, but very likely originates from platelets because a very low dose of acetylsalicylic acid (250 to 500 mg every other day, which irreversibly inhibits platelet cyclooxygenase, is highly effective in the prevention of erythromelalgia in thrombocythaemia.

  4. 非酒精性脂肪性肝病胰岛素抵抗程度与全血细胞计数的相关性%Correlation between insulin resistance and complete blood count parameters in nonalcoholic fatty liver disease

    Institute of Scientific and Technical Information of China (English)

    李静; 周利霞; 王俊明; 贾敏; 卢亚敏; 帖彦清

    2012-01-01

    目的 探讨伴或不伴2型糖尿病(T2DM)的非酒精性脂肪性肝病(NAFLD)胰岛素抵抗(IR)程度与全血细胞计数各参数的相关性.方法 选取糖耐量正常并除外糖尿病史的单纯NAFLD患者102例,T2DM合并NAFLD患者104例,正常对照104例为研究对象,测定空腹血糖(FPG)、空腹胰岛素(FINS)和全血细胞计数,分析胰岛素抵抗指数(HOMA-IR)与全血细胞计数各参数的相关性.结果 T2DM合并NAFLD患者IR及全血细胞计数异常程度较单纯NAFLD患者更重;相关性研究表明男性HOMA-IR与WBC、NEU、LYM、RBC、HGB、HCT呈正相关,女性HOMA-IR与WBC、NEU、LYM、MID、RBC、HGB、HCT呈正相关.结论 NAFLD时白细胞参数和红细胞参数的变化与IR密切相关,T2DM的存在加重了IR对上述血细胞参数的影响.全血细胞计数可以作为反映NAFLD患者IR程度的一种简单实用的检验指标.%Objective To investigate the correlation between insulin resistance (IR) and complete blood count parameters in nonalcoholic fatty liver disease ( NAFLD) with or without type 2 diabetes mellituB (T2DM). Methods 102 NAFLD patients without T2DM, 104 NAFLD patients with T2DM and 104 healthy controls were selected. Fasting plasma glucose (FPG) ,fasting insulin (FINS) levels and complete blood count parameters were measured. The degree of insulin resistance was assessed using the homeostasis model assessment ( HOMA). Correlation between HOMA-IR and complete blood count parameters were analyzed. Results IR and complete blood count parameters of NAFLD patients with T2DM showed more abnormal than NAFLD patients without T2DM. The correlation studies indicated that HOMA-IR in male was positively related to WBC, NEU, LYM, RBC, HGB and HCT,while in female,it was also positively related to WBC, NEU,LYM,MID,RBC,HGB and HCT. Conclusions Changes of WBC and RBC parameters are closely related to the degree of IR in NAFLD. NAFLD complicated with T2DM aggravates the impact of IR on the parameters

  5. Blood Types

    Science.gov (United States)

    ... maternity. Learn About Blood Blood Facts and Statistics Blood Components Whole Blood and Red Blood Cells Platelets Plasma ... About Blood Blood Facts and Statistics Blood Types Blood Components What Happens to Donated Blood Blood and Diversity ...

  6. Relationship between leukocyte count and angiographical characteristics of coronary atherosclerosis

    Institute of Scientific and Technical Information of China (English)

    En-zhi JIA; Zhi-jian YANG; Biao YUAN; Xiao-ling ZANG; Rong-hu WANG; Tie-bing ZHU; Lian-sheng WANG; Bo CHEN; Wen-zhu MA

    2005-01-01

    Aim: To explore the relationship between differential leucocyte count and coronary atherosclerosis. Methods: The study population consisted of 507 consecutive patients (376 male and 131 female) who underwent coronary angiography for suspected or known coronary atherosclerosis. The patients' smoking and drinking habits were investigated, and anthropometric measurements, serum measurements, and hematological measurements were conducted for every patient.The severity of coronary atherosclerosis was defined by using Gensini' s score system. One-way ANOVA, Spearman's correlation analysis, and multivariate stepwise linear regression analysis were employed to explore the relationship between differential leucocyte count and coronary atherosclerosis. Results: Oneway ANOVA indicated that the diastolic blood pressure, glucose, urea, creatinine,leukocyte count, neutrophil count, monocyte count, hemoglobin, and platelet count differed among the groups according to Gensini's score, the tertile values of which were used as cutoff points. Spearman's correlation analysis suggested that Gensini's score was significantly correlated with age, diastolic blood pressure,glucose, urea, creatinine, leukocyte count, neutrophil count, monocyte count,hemoglobin, and erythrocyte count, respectively. Multivariate stepwise linear regression analysis show that neutrophil count (β=0.247, P=0.000), age (β=0.141,P=0.001), glucose (β=0.173, P=0.000), creatinine (β=0.088, P=0.063), hemoglobin (β=-0.168, P=0.013) and sex (men were coded as 1 and women were coded as 2;β=-0.121, P=0.012) were significantly independently associated with the Gensini's score. Conclusion: The independent association of neutrophil count with the angiographical characteristics of coronary atherosclerosis, as estimated by Gensini's score, strongly suggests that granulocytosis may play a role in the development of coronary atherosclerosis.

  7. Correlative analysis of serum high sensitivity C-reactive protein and white blood cell count with coronary lesion and risk factors%白细胞计数和血清高敏C-反应蛋白与冠状动脉病变及危险因素相关性分析

    Institute of Scientific and Technical Information of China (English)

    冯姗

    2012-01-01

    Objective To investigate the relationship of high-sensitivity C-reactive protein (hsCRP) and white blood cell count (WBC) with extent of coronary lesion and if it is an independent risk factor for coronary heart disease (CHD).Methods One hundred and twelve patients with CHD confirmed by angiography and 35 patients without CHD (excluded by coronarography).All patients measuring WBC,serum hs-CRP,serum high density lipoprotein (HDL-C),low density lipoprotein (LDL-C),and 2 h postprandial blood glucose levels by automated hematology analyzer (AHA) and latex immune enhancement act.According to the results of coronary angiography,CHD group was divided into 1,2,3 lesions,observed the relationship between WBC and hs-CRP with coronary artery lesions.Also investigated age,gender,body mass index (BMI),blood pressure,smoking.Results There were significantlydifference (P < 0.01) between two groups in WBC,serum of hs-CRP,HDL-C,LDL-C,postprandial 2 h blood glucose levels.With the increase in the number of coronary arteries,the WBC count,serum hs-CRP levels gradually increased.White blood cell count and body mass index,waist circumference,blood pressure,HDL-C and LDL-C,2-hour postprandial blood glucose were positively correlated (P < 0.01),they were negatively correlated with high density lipoprotein (P =0.000).White blood cell count and severity of coronary lesions were positively correlated (r =0.400,P =0.000).The level of white blood cell count as the dependent variable multiple regression analysis showed that the only variable,BMI,of hs-CRP,smoking into the regression equation.Conclusions Inflammatory response may be involved in the occurrence of coronary artery disease,white blood cell count levels are related with cardiovascular disease risk factors such as obesity,2 h postprandial blood glucose,blood pressure,LDL and smoking.%目的 探讨白细胞计数(WBC)和超敏C-反应蛋白(hs-CRP)与冠状动脉病变程度的关系及是否是冠心病(CHD

  8. The right to count does not always count

    DEFF Research Database (Denmark)

    Sodemann, Morten

    2013-01-01

    The best prescription against illness is learning to read and to count. People who are unable to count have a harder time learning to read. People who have difficulty counting make poorer decisions, are less able to combine information and are less likely to have a strategy for life...

  9. CalCOFI Egg Counts

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Fish egg counts and standardized counts for eggs captured in CalCOFI icthyoplankton nets (primarily vertical [Calvet or Pairovet], oblique [bongo or ring nets], and...

  10. 21 CFR 864.8185 - Calibrator for red cell and white cell counting.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Calibrator for red cell and white cell counting... Calibrator for red cell and white cell counting. (a) Identification. A calibrator for red cell and white cell counting is a device that resembles red or white blood cells and that is used to set instruments...

  11. Determination of normal reference ranges for venous blood count among 526 children aging from 1year old to 12 years old in Shanghai%上海市526例1~12岁儿童静脉血血常规正常参考区间建立

    Institute of Scientific and Technical Information of China (English)

    高原; 邹琛; 蒋婕; 杨剑敏; 田先敏

    2015-01-01

    Objective To determine normal reference ranges for venous blood count among children aging from 1 year old to 12 years old.Methods These normal reference ranges were defined in a population of 526 healthy children who had no blood system diseases,allergic diseases,respiratory system diseases,urinary system diseases,digestive system disease,rheumatoid disease,thyroid disease,parasitic infections,malignancies and genetic disease,etc.Values of white blood cell count (WBC),red blood cell count (RBC),hemoglobin (Hb)concentration,red blood cell specific volume (Hct),mean corpusular volume(MCV),mean cell hemoglo-bin (MCH),mean corpuscular hemoglobin(MCHC),platelet (PLT),percentage of neutrophil (NE%),percentage of lymphocyte (LY%),percentage of mononuclear cells (MO%),percentage of acidophilic granulocyte (EOS%).Statistical analysis was done on various parameters that we recorded,and then for every parameter,we could get the various reference ranges for different age groups.Results The subjects were divided into 4 groups based on age.Besides the parameters of WBC count and classification of WBC,the rest of parameters were proved to be of no statistical difference between 4 groups..After an integration of the values,we could get the results as follows:RBC(4.02-5.2)×10 1 2/L,HGB 108-144 g/L,Hct 35.2%-40.4%,MCV 74.6-89.9 fL,MCH 20.9-34.7 pg,MCHC 332- 340 g/L,PLT(157 - 409 )× 10 9/L.WBC count did not have statistical difference between the age group 6-0.05),合并后得到 RBC:(4.02~5.2)×1012/L,HGB:108~144 g/L, Hct:35.2%~40.4%,MCV:74.6~89.9 fL,MCH:20.9~34.7 pg,MCHC:332~340 g/L,PLT:(157~409)×109/L。WBC 除6~0.05),其余各组间差异有统计学意义(P <0.05),经合并后得到1~<3岁组:(4.88~13.38)×109/L,3~<6岁组:(4.26~11.6)×109/L,6~12岁组:(4.24~10.24)×109/L。WBC 分类结果在不同年龄段差异有统计学意义(P <0.05),1~岁 NE:29%~32%,LY:58%~61%;3~<6岁组 NE:43%~46%,LY:43%~46%;6~<9岁组 NE:49%~52%,LY:38%~40%;9

  12. 血清降钙素原、C反应蛋白及白细胞计数联合检测对早期诊断沙门菌血流感染的价值研究%The value research of serum procalcitonin,C-reactive protein and white blood cell count combined detection of early diagnosis of Salmonella sense blood infectious diseases

    Institute of Scientific and Technical Information of China (English)

    蒋姣姣; 李怡霖

    2015-01-01

    目的:探讨血清降钙素原、C反应蛋白及白细胞计数联合检测对早期诊断沙门菌血流感染的价值.方法:对80例沙门菌血流染性疾病患者,60例非沙门菌血流感染性疾病患者分别检测各组的血清降钙素原、C-反应蛋白、白细胞计数值,比较其均值并确定其最佳诊断临界值.结果:沙门菌血流感染组的PCT、CRP、WBC均低于非沙门菌血流感染组,差异均有统计学意义(P<0.01).结论:对血流感染的患者进行血清PCT、CRP、WBC联合检测,可以对沙门菌血流感染做出早期、准确的诊断.%Objective:To discuss the value of serum procalcitonin,C-reactive protein and white blood cell count combined detection of early diagnosis of Salmonella sense blood infectious diseases.Methods:In 80 cases of Salmonella sense blood infectious diseases,60 cases of non Salmonella bloodstream infections disease patients were detected in each group of serum procalcitonin,C-reactive protein,white cell count compared to the average value and to determine the optimal diagnostic critical value.Results:Salmonella bloodstream infection groups PCT,CRP,WBC were lower than the non Salmonella bloodstream infection group,there was statistically significant(P<0.01).Conclusion:Bloodstream infections in patients with serum PCT,CRP,WBC joint detection can make early diagnosis,and accurate Salmonella bloodstream infection.

  13. Counting and Topological Order

    Institute of Scientific and Technical Information of China (English)

    陈阳军

    1997-01-01

    The counting method is a simple and efficient method for processing linear recursive datalog queries.Its time complexity is bounded by O(n,e)where n and e denote the numbers the numbers of nodes and edges,respectively,in the graph representing the input.relations.In this paper,the concepts of heritage appearance function and heritage selection function are introduced,and an evaluation algorithm based on the computation of such functions in topological order is developed .This new algorithm requires only linear time in the case of non-cyclic data.

  14. VARIABILITY OF LEUKOCYTE COUNT AS AN INFLAMMATORY RESPONSE AMONG HYPERTENSIVES

    Directory of Open Access Journals (Sweden)

    Brinda

    2015-12-01

    Full Text Available INTRODUCTION Hypertension, a globally prevailing non communicable disease has been linked to various pathophysiological mechanisms. The new spotlight is on the inflammatory mechanism, which has been embarked on this study by utilising the White blood cell counts as the predominant marker. The pathogenesis behind this concept is the endothelial alteration which occurs in hypertension, causing increased oxidative stress and release of inflammatory mediators and further causing damage to the vascular walls and may result in sustained hypertension. OBJECTIVE To substantiate the difference in leukocyte count among the hypertensives and normotensives, to assess the variation in the leukocyte count among varied grades of hypertension. METHODS 80 subjects and 40 controls of both sex and age between 20-60 years were taken. Subjects with other chronic medical disorders, pregnancy, menstruation phase, history of infections in past 3 months, obesity, smokers and regular alcoholics were excluded. Three recordings of blood pressure were measured with a standard mercury sphygmomanometer. The differential leukocyte count was estimated with an automated counter. RESULTS Statistical analysis was done using independent sample t-test and one-way ANOVA. Total Leukocyte count (Mean = 8397.50 ± 1666.57 was raised significantly among the hypertensives, with P value <0.001. Comparison was done on the total count with the varied hypertensive years, which was suggestive of increase in total count among the pre hypertensives (systolic pressure and there was linear increase in total count with diastolic pressure though not statistically significant. CONCLUSION The elevation of white blood cell count is suggestive of underlying inflammatory mechanism among the hypertensives. The onset of inflammation could be at the onset of hypertension, as there is rise in total leukocyte count among the pre hypertensives which could also lead to sustained hypertension.

  15. SysmexXE-5000血液分析仪与人工显微镜镜检白细胞分类相关性研究%Study on the correlation between SysmexXE-5000 Hematology Analyzer and artificial microscopy of white blood cell differential count

    Institute of Scientific and Technical Information of China (English)

    蒋叙川; 陈雅萍

    2015-01-01

    Objective To study the correlation between the Sysmex XE-5000 Automatic Hematology Analyzer and microscopy of white blood cell differential count.Methods A total of 100 blood samples were collected from Jianyang People′s Hospital,Sys-mex XE-5000 Automatic Hematology Analyzer was used to do white blood cell differential count,and the inter-batch precision,be-tween-run precision were calculated,and the correlation of the blood cell differential count detected by Sysmex XE-5000 Automatic Hematology Analyzer and artificial microscopy were analyzed.Results The inter-batch precision and between-run precision of Sys-mex XE-5000 Automatic Hematology Analyzer were within the allowable ranges,and these results had good correlation between SysmexXE-5000 Hematology Analyzer and artificial microscopy,the correlation coefficients of Neutrophils,Lymphocytes,Mono-cytes,Eosinophil were 0.978 2,0.909 5,0.827 0,0.868 6 (P 0.05).Conclusion Sysmex XE-5000 Automatic Hematology Analyzer for white blood cell differential is rapid,accurate and had better repeatability,and is suitable for detecting the quantity of samples differential,but it is not able to replace the manual micros-copy completely,when the samples have instrument flag,we will be required to perform artificial microscopy in order to improve the accuracy of white blood cell differential count.%目的:研究Sysmex XE-5000全自动血液分析仪与人工显微镜镜检白细胞分类的相关性。方法收集该院100份住院及体检者全血标本,采用 SysmexXE-5000全自动血液分析仪对高、中、低浓度白细胞标本进行白细胞分类,计算仪器法的批内、批间精密度,并与人工显微镜镜检白细胞分类结果进行相关性分析。结果SysmexXE-5000全自动血液分析仪白细胞分类批内及批间精密度均在允许范围内,且其白细胞分类结果与人工显微镜镜检结果具有良好的相关性,中性粒细胞、淋巴细胞、单核细胞、嗜酸粒

  16. The human megakaryocytic cell line UT-7/TPO expresses functional platelet agonist signals mediated through GPVI and thromboxane receptor.

    Science.gov (United States)

    Kawaguchi, Tatsuya; Hashimoto, Ryuji; Yokota, Hiroshi

    2010-09-01

    We have demonstrated that a unique megakaryocytic cell line UT-7/TPO could respond to one of the primary platelet signals through GP (glycoprotein) VI and a secondary signal of the AA (arachidonic acid) cascade. Unlike other megakaryocytic cell lines, UT-7/TPO was found to express GPVI and its associate signal molecule of FcRgamma (Fc receptor gamma chain). When UT-7/TPO was stimulated with the GPVI agonist convulxin, the [Ca2+]i (intracellular Ca2+) was elevated in a convulxin concentration-dependent manner, and [Ca2+]i elevation was blocked by pretreatment with the Src family kinase inhibitor PP2 and the phospholipase inhibitor U73122. These results strongly indicate that endogenously expressed GPVI signal molecules are functional in UT-7/TPO. Concerning the AA cascade, the expression of COX (cyclooxygenase)-1 and TX (thromboxane) synthase was observed, and this cell line was able to produce TX by exogenous AA, followed by [Ca2+]i elevation mediated through the TX receptor. It is worth noting that convulxin stimulation did not cause TX generation, even through the GPVI pathway and the AA cascade are functional in this cell line. As there are many reports that convulxin-stimulated platelets failed to produce TX, it is suggested that UT-7/TPO has the same property as the platelets in regards to convulxin stimulation. Thus, UT-7/TPO is useful for the observation of both the GPVI pathway and AA cascade without requiring either the induction of differentiation or GPVI transfection. Furthermore, this cell line provides a new tool for research on platelet activation signals.

  17. The Copenhagen primary care differential count (CopDiff) database

    DEFF Research Database (Denmark)

    Andersen, Christen Bertel L; Siersma, V.; Karlslund, W.

    2014-01-01

    BACKGROUND: The differential blood cell count provides valuable information about a person's state of health. Together with a variety of biochemical variables, these analyses describe important physiological and pathophysiological relations. There is a need for research databases to explore...... Practitioners' Laboratory has registered all analytical results since July 1, 2000. The Copenhagen Primary Care Differential Count database contains all differential blood cell count results (n=1,308,022) from July 1, 2000 to January 25, 2010 requested by general practitioners, along with results from analysis...... the construction of the Copenhagen Primary Care Differential Count database as well as the distribution of characteristics of the population it covers and the variables that are recorded. Finally, it gives examples of its use as an inspiration to peers for collaboration....

  18. Oscillations in counting statistics

    CERN Document Server

    Wilk, Grzegorz

    2016-01-01

    The very large transverse momenta and large multiplicities available in present LHC experiments on pp collisions allow a much closer look at the corresponding distributions. Some time ago we discussed a possible physical meaning of apparent log-periodic oscillations showing up in p_T distributions (suggesting that the exponent of the observed power-like behavior is complex). In this talk we concentrate on another example of oscillations, this time connected with multiplicity distributions P(N). We argue that some combinations of the experimentally measured values of P(N) (satisfying the recurrence relations used in the description of cascade-stochastic processes in quantum optics) exhibit distinct oscillatory behavior, not observed in the usual Negative Binomial Distributions used to fit data. These oscillations provide yet another example of oscillations seen in counting statistics in many different, apparently very disparate branches of physics further demonstrating the universality of this phenomenon.

  19. Clinical significance of serum myocardial enzyme spectrum, C-reactive protein and blood platelet count in diagnosis of children bronchopneumonia%血清心肌酶谱、C-反应蛋白和血小板计数变化在儿童支气管肺炎诊断中的临床意义

    Institute of Scientific and Technical Information of China (English)

    冼中任; 陈燕

    2015-01-01

    Objective To research clinical significance of serum myocardial enzyme spectrum, C-reactive (CRP) protein and blood platelet count (PLT) in diagnosis of children bronchopneumonia. Methods There were 175 children with bronchopneumonia as experimental group, Among them, there were 100 mild cases, 75 severe cases, and 110 bacteria-type cases, 50 virus-type cases, 15 microbe-type cases. There were another 100 healthy children as control group. Their changed serum myocardial enzyme spectrum was examined by enzyme kinetics, changed C-reactive protein was detected by immunity transmission turbidity, and changed blood platelet count was detected by blood routine test, and all received statistical analysis.Results The experimental group had all higher serum myocardial enzyme spectrum, C-reactive protein and blood platelet count than the control group. These three indexes of severe cases were all higher than mild cases. CRP was increased in bacteria-type, and their difference had statistical significance (P<0.05).Conclusion Changes of serum myocardial enzyme spectrum, C-reactive protein and blood platelet count is helpful for diagnosis of children bronchopneumonia. This method contains high sensitivity, wide feasibility, convenience and economical advantage, and it is worthy of clinical promotion.%目的 研究血清心肌酶谱、C-反应蛋白(CRP)和血小板计数(PLT)变化在儿童支气管肺炎诊断中的临床意义.方法 选取175例支气管肺炎患儿, 其中轻型100例, 重型75例;细菌型110例, 病毒型50例, 微生物型15例.并选取100例正常儿童作为对照.均使用酶动力学检查血清心肌酶谱变化;选择免疫透射比浊法测定C-反应蛋白变化;血常规测定血小板计数的变化, 并进行统计分析.结果 支气管肺炎患儿血清心肌酶谱、C-反应蛋白和血小板计数均高于正常儿童;重型支气管肺炎患儿三项指标均高于轻型;细菌型CRP显著增高, 差异均具有统计学意义(P<0.05).

  20. Electrical cell counting process characterization in a microfluidic impedance cytometer.

    Science.gov (United States)

    Hassan, Umer; Bashir, Rashid

    2014-10-01

    Particle counting in microfluidic devices with coulter principle finds many applications in health and medicine. Cell enumeration using microfluidic particle counters is fast and requires small volumes of sample, and is being used for disease diagnostics in humans and animals. A complete characterization of the cell counting process is critical for accurate cell counting especially in complex systems with samples of heterogeneous population interacting with different reagents in a microfluidic device. In this paper, we have characterized the electrical cell counting process using a microfluidic impedance cytometer. Erythrocytes were lysed on-chip from whole blood and the lysing was quenched to preserve leukocytes which subsequently pass through a 15 μm × 15 μm measurement channel used to electrically count the cells. We show that cell counting over time is a non-homogeneous Poisson process and that the electrical cell counts over time show the log-normal distribution, whose skewness can be attributed to diffusion of cells in the buffer that is used to meter the blood. We further found that the heterogeneous cell population (i.e. different cell types) shows different diffusion characteristics based on the cell size. Lymphocytes spatially diffuse more as compared to granulocytes and monocytes. The time difference between the cell occurrences follows an exponential distribution and when plotted over time verifies the cell diffusion characteristics. We also characterized the probability of occurrence of more than one cell at the counter within specified time intervals using Poisson counting statistics. For high cell concentration samples, we also derived the required sample dilution based on our particle counting characterization. Buffer characterization by considering the size based particle diffusion and estimating the required dilution are critical parameters for accurate counting results.

  1. Platelet count in healthy subjects treated with antiplatelet drugs

    Directory of Open Access Journals (Sweden)

    Maczy González-Rincón

    2016-07-01

    Full Text Available Platelet count in peripheral blood of healthy subjects with antiplatelet drugs. 20 subjects were analized. They were distributed in two groups: subject A: 10 who received aspirin (100 mg and B:10 with Clopidogrel (75 mg for 7 days. In all subjects studied platelet count in peripheral blood and PRP. It found a platelet count before treatment with antiplatelet agents in peripheral blood of 258,6 ± 54,46 x 109 l and 7 days after 254 ± 41,86 x 109 l (aspirin and 285,4 ± 70, 196,5 ± 37,90 x 109 l (Clopidogrel respectively. In the PRP of subjects before receiving aspirin was 486,5 ± 129,54 x 109 l and after 449,2 ± 85,51 x 109 l; prior to Clopidogrel ingestion was 565,2 ± 150,41 and 592,9 ± 203,46 x 109 l after treatment. Significant differences were found only for the platelet count in the Clopidogrel Group (p < 0.05. A significant decrease in platelet count was observed in peripheral blood after administration of Clopidogrel, possibly as a result of its pharmacological mechanism. More studies are needed to assess a greater number of individuals and better measure the effect of antiplatelet agents.

  2. Multivariate ultrametric root counting

    CERN Document Server

    Avendano, Martin

    2011-01-01

    Let $K$ be a field, complete with respect to a discrete non-archimedian valuation and let $k$ be the residue field. Consider a system $F$ of $n$ polynomial equations in $K\\vars$. Our first result is a reformulation of the classical Hensel's Lemma in the language of tropical geometry: we show sufficient conditions (semiregularity at $w$) that guarantee that the first digit map $\\delta:(K^\\ast)^n\\to(k^\\ast)^n$ is a one to one correspondence between the solutions of $F$ in $(K^\\ast)^n$ with valuation $w$ and the solutions in $(k^\\ast)^n$ of the initial form system ${\\rm in}_w(F)$. Using this result, we provide an explicit formula for the number of solutions in $(K^\\ast)^n$ of a certain class of systems of polynomial equations (called regular), characterized by having finite tropical prevariety, by having initial forms consisting only of binomials, and by being semiregular at any point in the tropical prevariety. Finally, as a consequence of the root counting formula, we obtain the expected number of roots in $(K...

  3. Resolved SZE Cluster Count

    Institute of Scientific and Technical Information of China (English)

    Jia-Yu Tang; Zu-Hui Fan

    2003-01-01

    We study the counts of resolved SZE (Sunyaev-Zel'dovich effect) clus-ters expected from an interferometric survey in different cosmological models underdifferent conditions. The self-similar universal gas model and Press-Schechter massfunction are used. We take the observing frequency to be 90 GHz, and consider twodish diameters, 1.2 m and 2.5 m. We calculate the number density of the galaxyclusters dN/(dΩdz) at a high flux limit Slimv = 100mJy and at a relative lowSlimv = 10 mJy. The total numbers of SZE clusters N in two low-Ω0 models arecompared. The results show that the influence of the resolved effect depends notonly on D, but also on Slimv: at a given D, the effect is more significant for a highthan for a low Slim Also, the resolved effect for a flat universe is more impressivethan that for an open universe. For D = 1.2m and Slimv= 10mJy, the resolvedeffect is very weak. Considering the designed interferometers which will be used tosurvey SZE clusters, we find that the resolved effect is insignificant when estimatingthe expected yield of the SZE cluster surveys.

  4. Making environmental DNA count.

    Science.gov (United States)

    Kelly, Ryan P

    2016-01-01

    The arc of reception for a new technology or method--like the reception of new information itself--can pass through predictable stages, with audiences' responses evolving from 'I don't believe it', through 'well, maybe' to 'yes, everyone knows that' to, finally, 'old news'. The idea that one can sample a volume of water, sequence DNA out of it, and report what species are living nearby has experienced roughly this series of responses among biologists, beginning with the microbial biologists who developed genetic techniques to reveal the unseen microbiome. 'Macrobial' biologists and ecologists--those accustomed to dealing with species they can see and count--have been slower to adopt such molecular survey techniques, in part because of the uncertain relationship between the number of recovered DNA sequences and the abundance of whole organisms in the sampled environment. In this issue of Molecular Ecology Resources, Evans et al. (2015) quantify this relationship for a suite of nine vertebrate species consisting of eight fish and one amphibian. Having detected all of the species present with a molecular toolbox of six primer sets, they consistently find DNA abundances are associated with species' biomasses. The strength and slope of this association vary for each species and each primer set--further evidence that there is no universal parameter linking recovered DNA to species abundance--but Evans and colleagues take a significant step towards being able to answer the next question audiences tend to ask: 'Yes, but how many are there?'

  5. SUMS Counts-Related Projects

    Data.gov (United States)

    Social Security Administration — Staging Instance for all SUMs Counts related projects including: Redeterminations/Limited Issue, Continuing Disability Resolution, CDR Performance Measures, Initial...

  6. Role of eicosanoids and white blood cells in the beneficial effects of limited reperfusion after ischemia-reperfusion injury in skeletal muscle

    Energy Technology Data Exchange (ETDEWEB)

    Anderson, R.J.; Cambria, R.A.; Dikdan, G.; Lysz, T.W.; Hobson, R.W. II (UMDNJ-New Jersey Medical School, Newark (USA))

    1990-08-01

    Limiting the rate of reperfusion blood flow has been shown to be beneficial locally in models of ischemia-reperfusion injury. We investigated the effects of this on eicosanoids (thromboxane B2, 6-keto-PGF1 alpha, and leukotriene B4), white blood cell activation, and skeletal muscle injury as quantitated by triphenyltetrazolium chloride and technetium-99m pyrophosphate after ischemia-reperfusion injury in an isolated gracilis muscle model in 16 anesthetized dogs. One gracilis muscle in each dog was subjected to 6 hours of ischemia followed by 1 hour of limited reperfusion and then by a second hour of normal reperfusion. The other muscle was subjected to 6 hours of ischemia followed by 2 hours of normal reperfusion. Six dogs each were used as normal reperfusion controls (NR) and limited reperfusion controls (LR), with 5 dogs being treated with a thromboxane synthetase inhibitor (LR/TSI) and another five with a leukotriene inhibitor (LR/LI). LR in all three groups (LR, LR/TSI, and LR/LI) showed a benefit in skeletal muscle injury as measured by triphenyltetrazolim chloride and technetium-99m pyrophosphate when compared with NR. However, there was no significant difference between the groups with LR regarding eicosanoid levels and white blood cell activation when compared with NR. These results demonstrate that LR produces benefits by mechanisms other than those dependent upon thromboxane A2, prostacyclin, or white blood cell activation.

  7. Thromboxane A{sub 2} receptor signaling promotes liver tissue repair after toxic injury through the enhancement of macrophage recruitment

    Energy Technology Data Exchange (ETDEWEB)

    Minamino, Tsutomu [Departments of Pharmacology, Kitasato University School of Medicine, Kanagawa 252-0374 (Japan); Departments of Gastroenterology, Kitasato University School of Medicine, Kanagawa 252-0374 (Japan); Ito, Yoshiya [Departments of Surgery, Kitasato University School of Medicine, Kanagawa 252-0374 (Japan); Ohkubo, Hirotoki [Departments of Pharmacology, Kitasato University School of Medicine, Kanagawa 252-0374 (Japan); Departments of Surgery, Kitasato University School of Medicine, Kanagawa 252-0374 (Japan); Hosono, Kanako; Suzuki, Tatsunori [Departments of Pharmacology, Kitasato University School of Medicine, Kanagawa 252-0374 (Japan); Sato, Takehito [Departments of Pharmacology, Kitasato University School of Medicine, Kanagawa 252-0374 (Japan); Departments of Gastroenterology, Kitasato University School of Medicine, Kanagawa 252-0374 (Japan); Ae, Takako; Shibuya, Akitaka [Departments of Gastroenterology, Kitasato University School of Medicine, Kanagawa 252-0374 (Japan); Sakagami, Hiroyuki [Departments of Anatomy, Kitasato University School of Medicine, Kanagawa 252-0374 (Japan); Narumiya, Shuh [Department of Pharmacology, Kyoto University School of Medicine, Kyoto, 606-8315 (Japan); Koizumi, Wasaburo [Departments of Gastroenterology, Kitasato University School of Medicine, Kanagawa 252-0374 (Japan); Majima, Masataka, E-mail: mmajima@med.kitasato-u.ac.jp [Departments of Pharmacology, Kitasato University School of Medicine, Kanagawa 252-0374 (Japan)

    2012-02-15

    It is thought that thromboxane A{sub 2} (TxA{sub 2}) contributes to the progression of inflammation during acute hepatic injury; however, it is still unknown whether TxA{sub 2} is involved in liver repair. The objective of the present study was to examine the role of TxA{sub 2} receptor (TP) signaling in liver injury and repair in response to toxic injury. Carbon tetrachloride (CCl{sub 4}) was used to induce liver injury in TP knockout (TP{sup −/−}) mice and wild-type (WT) mice. In WT mice, serum levels of alanine aminotransferase (ALT) and the size of the necrotic area peaked at 24 and 48 h, respectively, and then declined. In TP{sup −/−} mice, the changes in ALT levels were similar to WT mice, but liver regeneration was impaired as evidenced by remained elevated levels of hepatic necrosis and by delayed hepatocyte proliferation, which was associated with the reduced expression of growth factors including interleukin-6 (IL-6), tumor necrosis factor alpha (TNFα), and hepatocyte growth factor (HGF). In TP{sup −/−} mice, the accumulation of hepatic CD11b{sup +}/F4/80{sup +} macrophages in injured livers was attenuated, and the hepatic expression of monocyte chemoattractant protein-1 (MCP-1/CCL2) and its receptor, the C―C chemokine receptor (CCR2), was reduced compared to WT. Additionally, the application of the TP receptor agonist, U-46619, enhanced the expression of MCP-1/CCL2 and CCR2 in peritoneal macrophages, which was associated with increased levels of IL-6, TNFα and HGF. These results suggested that TP receptor signaling facilitates liver recovery following CCl{sub 4}-induced hepatotoxicity by affecting the expression of hepatotrophic growth factors, and through the recruitment of macrophages mediated by MCP-1/CCL2-CCR2 expression. -- Highlights: ► TP enhances liver regeneration by CCl{sub 4}. ► TP accumulates macrophages. ► TP up-regulates MCP-1.

  8. Effects of Replenishing Qi, Promoting Blood Circulation and Resolving Phlegm on Vascular Endothelial Function and Blood Coagulation System in Senile Patients with Hyperlipemia

    Institute of Scientific and Technical Information of China (English)

    Yang Huimin; Han Libei; Sheng Tong; He Qiong; Liang Jinpu

    2006-01-01

    Objective: To observe the curative effect of the method of replenishing qi, promoting blood circulation and resolving phlegm on senile hyperlipemia and its effects on vascular endothelial function and blood coagulation system. Method: 96 patients with senile hyperlipemia were randomly divided into a treatment group and a of blood lipid, vascular endothelial function, blood coagulation system and safety. Results: After treatment,the treatment group was obviously superior to the control group (P<0.05) in reducing plasma total cholesterol (TC) and low density lipoprotein cholesterol (LDL-C) as well as in the ratio of thromboxane B2 (TXB2) to 6-keto-prostaglandin F1α (6-keto-PGF1α), D-dimer (D-D) and fibrinogen (FIB). Conclusion: Danshen Jueming Granules have the effect of regulating metabolism of blood lipid, and improving vascular endothelial function and blood coagulation system in senile patients with hyperlipemia.

  9. Relationships of hepatic fibrosis stages, liver morphological changes with ultrasonography of type B, and peripheral blood cell counts in chronic hepatitis%慢性肝炎患者肝脏B超形态学、周围血细胞计数与肝纤维化分期之间的关系

    Institute of Scientific and Technical Information of China (English)

    刘贵生; 陈欣; 王萍; 袁农; 王宪智; 管湘平

    2005-01-01

    目的探讨慢性病毒性肝炎患者肝脏B超形态学改变、周围血细胞计数与肝纤维化病理分期之间的关系.方法对120例慢性病毒性肝炎患者行B超引导下肝活检及肝组织学纤维化分期;B超检查测定门静脉为主(MPV)、脾静脉(SPV)宽度及脾脏厚度;同时检测周围血细胞计数.比较上述指标之间的关系.结果120例慢性肝炎患者肝纤维化分期结果为:S0期15例,S1期40例,S2期25例,S3期24例,S4期16例.从S1~S4期,MPV、SPV增宽及脾脏增厚的比率逐渐上升,但MPV、SPV增宽的比率各期差别不大(P>0.05),而脾脏增厚的发生率各期差别较大(0.01<P<0.025).从S1~S4期,周围血红细胞(RBC)、白细胞(WBC)及血小板(PLT)计数逐渐下降,尤以PLT下降最明显,S3、S4期显著低于S1、S2期(P分别<0.05、0.01),其中S4期已在正常下限以下.RBC,WBC在S1、S2、S3期之间并无显著性差异(P>0.05),S4期则较前面各期显著下降(P分别<0.05,0.01).结论肝脏B超形态学改变、周围血细胞计数对慢性肝炎患者早期肝纤维化的诊断价值是有限的.脾脏肿大、SPV增宽、PLT计数下降可能对较严重的肝纤维化(S3或S4期)的诊断有一定作用.%Objective: To discuss the relationships of the hepatic fibrosis stages, liver morphological changes with ultrasonography of type B, and the peripheral blood cell counts in chronic hepatitis. Methods: In the series, 120patients with chronic viral hepatitis were undertaken liver biopsy and determinated the pathological stages of hepatic fibrosis. The diameters of main portal vein, splenic vein and the size of spleen were measured by ultrasonography of type B. In addition, the perpheral blood cell counts were tested routinely. Results: The results of different hepatic fibrosis stages were S0 15, S1 40, S2 25, S3 24, and S4 16. From S1 to S4, the ratios of broadening of main portal vein, splenic vein and spcenormegaly increased gradually. But there were no

  10. The influence of storage time and preserving temperature on infant children′s peripheral blood cell count%放置时间及保存温度对幼儿末梢全血细胞计数的影响

    Institute of Scientific and Technical Information of China (English)

    孙秋瑾; 王宇

    2011-01-01

    目的 探讨放置时间、保存温度对2~6岁幼儿末梢全血细胞计数结果的影响,以指导体检工作流程的合理安排.方法 取40名幼儿末梢全血用乙二胺四乙酸三钾(EDTA-K3)抗凝,分组标记后保存于不同温度中.应用ABX-Micros 60全自动血液分析仪,将分装标本分别在0.5、4、8和10 h测定.结果 保存于室温和4 ℃冰箱冷藏环境下,随着放置时间的延长,白细胞(WBC)、红细胞(RBC)、平均血小板体积(MPV)、血小板分布宽度(PDW)、淋巴细胞百分率(LY)均有逐渐上升的趋势;血小板(PLT)计数、中性粒细胞百分率(GRA)均有下降的趋势.标本于室温8 h 及4 ℃冷藏10 h以后检测结果与对照组比较,大多数指标差异均有统计学意义(P<0.05).10 h时室温与4 ℃冷藏放置2组数据比较,大多数指标差异有统计学意义(P<0.05).结论 标本放置时间及保存温度对幼儿末梢全血细胞计数结果中的一些参数有明显影响,在4 h之内检测,室温与4 ℃冷藏2种环境对检测结果无明显影响;末梢血标本放置在4 ℃冷藏条件下10 h内完成检测还是可行的.%Objective To investigate the influence of storage time and preserving temperature on 2-6 years old infant children's peripheral blood cell count, and improve the process of health examination. Methods 40 cases of infant children's peripheral blood specimens were anticoagulated by ethylenediamine tetraacetic acid-K3 (EDTA-K3 ).The blood specimens were classified and stored in different temperatures after being labeled. The blood specimens were determined by ABX-Mieros 60 at 0.5, 4,8 and 10 h respectively. Results The levels of white blood cell ( WBC), red blood cell (RBC), mean platelet volume (MPV), platelet distribution width (PDW), lymphocyte% (LY) were increased, and platelet (PLT) and nentrophilicgranulocyte % (GRA) were decreased with prolonging storage time in room temperature and refrigerator 4 ℃. After 8 h in room temperature and

  11. Coinductive counting with weighted automata

    NARCIS (Netherlands)

    Rutten, J.J.M.M.

    2002-01-01

    A general methodology is developed to compute the solution of a wide variety of basic counting problems in a uniform way: (1) the objects to be counted are enumerated by means of an infinite weighted automaton; (2) the automaton is reduced by means of the quantitative notion of stream bisimulation;

  12. Reference counting for reversible languages

    DEFF Research Database (Denmark)

    Mogensen, Torben Ægidius

    2014-01-01

    deallocation. This requires the language to be linear: A pointer can not be copied and it can only be eliminated by deallocating the node to which it points. We overcome this limitation by adding reference counts to nodes: Copying a pointer to a node increases the reference count of the node and eliminating...

  13. Hanford whole body counting manual

    Energy Technology Data Exchange (ETDEWEB)

    Palmer, H.E.; Brim, C.P.; Rieksts, G.A.; Rhoads, M.C.

    1987-05-01

    This document, a reprint of the Whole Body Counting Manual, was compiled to train personnel, document operation procedures, and outline quality assurance procedures. The current manual contains information on: the location, availability, and scope of services of Hanford's whole body counting facilities; the administrative aspect of the whole body counting operation; Hanford's whole body counting facilities; the step-by-step procedure involved in the different types of in vivo measurements; the detectors, preamplifiers and amplifiers, and spectroscopy equipment; the quality assurance aspect of equipment calibration and recordkeeping; data processing, record storage, results verification, report preparation, count summaries, and unit cost accounting; and the topics of minimum detectable amount and measurement accuracy and precision. 12 refs., 13 tabs.

  14. Multiple loci are associated with white blood cell phenotypes

    NARCIS (Netherlands)

    M.A. Nalls (Michael); D. Couper (David); T. Tanaka (Toshiko); F.J.A. van Rooij (Frank); M-H. Chen (Ming-Huei); A.V. Smith (Albert Vernon); D. Toniolo (Daniela); N.A. Zakai (Neil); Q. Yang (Qiong Fang); A. Greinacher (Andreas); A.R. Wood (Andrew); M. Garcia (Melissa); P. Gasparini (Paolo); Y. Liu (Yongmei); T. Lumley (Thomas); A.R. Folsom (Aaron); A.P. Reiner (Alex); C. Gieger (Christian); V. Lagou (Vasiliki); J.F. Felix (Janine); H. Völzke (Henry); N.A. Gouskova (Natalia); A. Biffi (Alessandro); A. Döring (Angela); U. Völker (Uwe); S. Chong (Sean); K.L. Wiggins (Kerri); A. Rendon (Augusto); A. Dehghan (Abbas); M. Moore (Matt); K.D. Taylor (Kent); J.G. Wilson (James); G. Lettre (Guillaume); A. Hofman (Albert); J.C. Bis (Joshua); N. Pirastu (Nicola); C.S. Fox (Caroline); C. Meisinger (Christa); J.G. Sambrook (Jennifer); S. Arepalli (Sampath); M. Nauck (Matthias); H. Prokisch (Holger); J. Stephens (Jonathan); N.L. Glazer (Nicole); L.A. Cupples (Adrienne); Y. Okada (Yukinori); A. Takahashi (Atsushi); Y. Kamatani (Yoichiro); K. Matsuda (Koichi); T. Tsunoda (Tatsuhiko); M. Kubo (Michiaki); Y. Nakamura (Yusuke); K. Yamamoto (Kazuhiko); M. Stumvoll (Michael); A. Tönjes (Anke); I. Prokopenko (Inga); T. Illig (Thomas); K.V. Patel (Kushang); S.F. Garner (Stephen); B. Kuhnel (Brigitte); M. Mangino (Massimo); B.A. Oostra (Ben); S.L. Thein; J. Coresh (Josef); H.E. Wichmann (Heinz Erich); S. Menzel (Stephan); J. Lin; G. Pistis (Giorgio); A.G. Uitterlinden (André); T.D. Spector (Timothy); A. Teumer (Alexander); G. Eiriksdottir (Gudny); V. Gudnason (Vilmundur); S. Bandinelli (Stefania); T.M. Frayling (Timothy); A. Chakravarti (Aravinda); P. Tikka-Kleemola (Päivi); D. Melzer (David); W.H. Ouwehand (Willem); D. Levy (Daniel); E.A. Boerwinkle (Eric); A. Singleton (Andrew); D.G. Hernandez (Dena); D.L. Longo (Dan); N. Soranzo (Nicole); J.C.M. Witteman (Jacqueline); B.M. Psaty (Bruce); L. Ferrucci (Luigi); T.B. Harris (Tamara); C.J. O'Donnell (Christopher); S.K. Ganesh (Santhi)

    2011-01-01

    textabstractWhite blood cell (WBC) count is a common clinical measure from complete blood count assays, and it varies widely among healthy individuals. Total WBC count and its constituent subtypes have been shown to be moderately heritable, with the heritability estimates varying across cell types.

  15. Blood sugar test - blood

    Science.gov (United States)

    ... blood glucose level ( hypoglycemia ) may be due to: Hypopituitarism (a pituitary gland disorder) Underactive thyroid gland or ... tonic-clonic seizure Glucagon blood test Glucagonoma Hyperthyroidism Hypopituitarism Hypothyroidism Insulinoma Low blood sugar Multiple endocrine neoplasia ( ...

  16. High Count Rate Single Photon Counting Detector Array Project

    Data.gov (United States)

    National Aeronautics and Space Administration — An optical communications receiver requires efficient and high-rate photon-counting capability so that the information from every photon, received at the aperture,...

  17. No effects of atorvastatin (10 mg/d or 80 mg/d) on nitric oxide, prostacyclin, thromboxane and oxidative stress in type 2 diabetes mellitus patients of the DALI study

    NARCIS (Netherlands)

    Tsikas, D.; Pham, V.V.; Suchy, M.T.; Ree, M.A. van de; Huisman, M.V.; Frolich, J.C.; Princen, H.M.; Hoogerbrugge, N.

    2015-01-01

    The present study describes the effects of atorvastatin on whole body synthesis of nitric oxide (NO), prostacyclin (PGI2), and thromboxane A2 (TxA2), on oxidative stress and nitrite/nitrate-related renal carbonic anhydrase (CA) activity in patients with type 2 diabetes mellitus (T2DM). A double-blin

  18. Vote Counting as Mathematical Proof

    DEFF Research Database (Denmark)

    Schürmann, Carsten; Pattinson, Dirk

    2015-01-01

    Trust in the correctness of an election outcome requires proof of the correctness of vote counting. By formalising particular voting protocols as rules, correctness of vote counting amounts to verifying that all rules have been applied correctly. A proof of the outcome of any particular election......-based formalisation of voting protocols inside a theorem prover, we synthesise vote counting programs that are not only provably correct, but also produce independently verifiable certificates. These programs are generated from a (formal) proof that every initial set of ballots allows to decide the election winner...

  19. Prognostic value of preoperative peripheral monocyte count in patients with hepatocellular carcinoma after liver transplantation.

    Science.gov (United States)

    Ren, Qing-Qi; Fu, Shun-Jun; Zhao, Qiang; Guo, Zhi-Yong; Ji, Fei; Chen, Mao-Gen; Wu, Lin-Wei; He, Xiao-Shun

    2016-07-01

    Prognostic value of peripheral monocyte, as a member of inflammatory cells, was widely being investigated. The aim of this study was to evaluate the prognostic value of preoperative peripheral blood monocyte count for hepatocellular carcinoma (HCC) patients who underwent liver transplantation (LT) and the relationship between monocyte count and tumor-related characteristics. We retrospectively analyzed the clinical data of 101 HCC patients after LT. Preoperative monocyte count and demographic, clinical, and pathologic data were analyzed. The optimal cutoff value of monocyte count was 456/mm(3), with the sensitivity and specificity of 69.4 and 61.5 %, respectively. Elevated preoperative peripheral blood monocyte count was significantly associated with large tumor size. The 1-, 3-, and 5-year disease-free survival (DFS) (80.9, 70.1, and 53.3 % vs 55.1, 38.7, and 38.7 %, P = 0.007) and overall survival (OS) rates (95.7, 76.6, and 64.8 % vs 72.2, 44.1, and 36.1 %, P = 0.002) of HCC patients in the peripheral blood monocyte count ≤456/mm(3) group were higher than those in the peripheral blood monocyte count >456/mm(3) group. In conclusion, elevated preoperative peripheral blood monocyte count was significantly associated with advanced tumor stage and it can be considered as a prognostic factor for HCC patients after LT.

  20. Make My Trip Count 2015

    Data.gov (United States)

    Allegheny County / City of Pittsburgh / Western PA Regional Data Center — The Make My Trip Count (MMTC) commuter survey, conducted in September and October 2015 by GBA, the Pittsburgh 2030 District, and 10 other regional transportation...

  1. 辽宁省沿海地区3170名男性部队官兵血常规的现况调查%A cross-sectional investigation on blood count of 3 170 male officers and soldiers from littoral army in Niaoning Province

    Institute of Scientific and Technical Information of China (English)

    向阳; 李海霞; 常晓慧; 张德杰; 成玉斌; 陈爱萍; 王晓波; 黄世林

    2013-01-01

    OBJECTIVE To investigate the current situation on blood count of the officers and soldiers from littoral army in Niaoning Province.METHODS The venous blood about 3ml/one person from the 3 170 male officers and soldiers were collected by cluster sampling method and the white blood cell (WBC),red blood cell (RBC),Hb and s platelets (PLT) were detected.RESULTS Among the 3 170 officers and soldiers,WBC,RBC,Hb,PLT were (6.6 ± 1.6) ×109/L,(5.3 ± 1.2) ×1012/L,(148.1 ± 13.8) g/L,(211.2 ± 52.1) ×109/L respectively.The abnormality detection rates of WBC,RBC,Hb,PLT were 5.6%,12.8%,14.2% and 6.2% respectively and Hb was highest among others.The abnormality detection rates of WBC,RBC,PLT form army was higher than navy and air-force while Hb form navy was higher than navy and airforce.CONCLUSIONS The chief indexes of blood count such as WBC,RBC,Hb,PLT of the officers and soldiers detected from littoral army maintain normal level.%目的 调查辽宁省沿海地区部队官兵血常规的现状.方法 采用整群抽样方法,采集3 170名陆海空男性官兵的静脉血2ml,检测白细胞(WBC)、红细胞(RBC)、血红蛋白(Hb)、血小板(PLT).结果 (1)受检官兵的WBC、RBC、Hb、PLT分别为(6.6±1.6)×109/L、(5.3±1.2)×1012/L、(148.1±13.8) g/L、(211.2±52.1)×109/L; (2) WBC、RBC、Hb和PLT异常检出率分别为5.6%、12.8%、14.2%和6.2%,其中Hb异常检出率居于首位; (3)陆军WBC、RBC、PLT的异常检出率高于海军和空军,分别为5.9%、14.2%、6.5%,Hb的异常检出率则以海军最高,为31.3%,其次为空军、陆军.结论 受检官兵WBC、RBC、Hb和PLT 4项血常规的主要指标均在正常检测范围.

  2. The Mayan Long Count Calendar

    OpenAIRE

    Chanier, Thomas

    2015-01-01

    The Maya were known for their astronomical proficiency. This is demonstrated in the Mayan codices where ritual practices were related to astronomical events/predictions. Whereas Mayan mathematics were based on a vigesimal system, they used a different base when dealing with long periods of time, the Long Count Calendar (LCC), composed of different Long Count Periods: the Tun of 360 days, the Katun of 7200 days and the Baktun of 144000 days. There were two other calendars used in addition to t...

  3. Counting Word Frequencies with Python

    Directory of Open Access Journals (Sweden)

    William J. Turkel

    2012-07-01

    Full Text Available Your list is now clean enough that you can begin analyzing its contents in meaningful ways. Counting the frequency of specific words in the list can provide illustrative data. Python has an easy way to count frequencies, but it requires the use of a new type of variable: the dictionary. Before you begin working with a dictionary, consider the processes used to calculate frequencies in a list.

  4. Changes of peripheral white blood cell count and platelet parameters in patients with acute renal failure and their clinical signiifcance%急性肾功能衰竭患者外周血白细胞计数和血小板参数值的变化及临床意义

    Institute of Scientific and Technical Information of China (English)

    刘喜龙; 谢琳瑛; 何志红

    2014-01-01

    Objective:To study the changes of peripheral white blood cell (WBC) count and platelet (PLT) parameters in patients with acute renal failure (ARF) and their clinical signiifcance. Methods:Sixty-seven patients with ARF were selected as an observation group and 65 cases of normal subjects as a control group, and peripheral WBC count and PLT parameters such as mean PLT volume (MPV), PLT volume distribution width (PDW), thrombocytocrit (PCT), and red cell distribution width (RDW) were detected by an automatic blood cell analysis method and ARF severity of patients were assessed by disease staging criteria in Kidney Disease Improvement Global Outcomes for Acute Kidney Injury. Results:Peripheral WBC count was signiifcantly higher while PLT, MPV, PDW and PCT were signiifcantly lower in the observation group than in the control group (P<0.05), however, the difference of RDW between two groups was not signiifcant. Peripheral WBC count, PLT, MPV, PDW, and PCT were obviously better in patients with stage I than with stage II, III and all the indexes were better in patients with stage II than with the stage III (P<0.05). Mortality accounted for 14.93%, infection rate 77.61%and bleeding rate 26.87%in the patients with ARF. Peripheral WBC count, PLT, MPV, PDW, and PCT were better in patients without complications than with complications (P<0.05). Conclusion: Detection of peripheral WBC count and PLT parameters play an important role in evaluation of the severity and prognosis of ARF.%目的:探讨急性肾功能衰竭(acute renal failure, ARF)患者外周血白细胞(white blood cell, WBC)计数和血小板(platelet, PLT)参数值的变化及临床意义。方法:选取67例ARF患者(观察组)和65例慢性肾炎患者(对照组)作为研究对象,采用全自动血细胞分析方法检测患者的外周血WBC计数和PLT参数值,包括PLT计数、平均PLT体积(mean platelet volume, MPV)、PLT体积宽度分布(platelet volume distribution

  5. Avaliação pós-operatória, através de exame clínico, laboratorial e ultra-sonográfico, de eqüinos submetidos à toracotomia experimental Post operatory evaluation of horses subjected to experimental thoracotomy by means of a complete clinical examination, blood gás analysis, complete blood count and thoracic ultrasonography

    Directory of Open Access Journals (Sweden)

    Raquel Yvonne Arantes Baccarm

    1998-12-01

    Full Text Available Nos trabalhos encontrados na literatura é dada pouca ênfase às conseqüências na cavidade torácica de eqüinos que são submetidos à toracotomia. Também não mencionam a ultra-sonografia como método auxiliar e de controle na avaliação pós-operatória, muitas vezes, optando-se exclusivamente pela necropsia. Neste experimento, foram utilizados seis eqüinos hígidos, sob anestesia com ventilação controlada, para a realização de acesso cirúrgico ao hemitórax direito, com o objetivo de se avaliarem através de exame clínico, mensuração de pH e gases sanguíneos, hemograma e ultra-sonografia torácica, as conse- qüências da prática de toracotomia em eqüinos. Não foram observados em nenhum dos animais transtornos respiratórios, sinais de pneumotórax ou deiscências de sutura de parede torácica. Os valores dos parâmetros fisiológicos, do pH e gases sanguíneos e do hemograma não apresentaram alterações significativas no pós-operatório. O exame ultra-sonográfico proporcionou informações sobre a quantidade e o aspecto do líquido pleural, evolução das alterações na pleura visceral, presença ou não de pneumotórax e alterações parenquimatosas subpleurais.Pertaining litterature does not usually foccus on the possible consequences in the thoracic cavity of horses subjected to thoracotomy procedures. Ultrasound is not mentioned as a method of contrai in post-operatory evaluation, which is mostiy exclusively based on post-mortem examination. Six healthy equine were used in the experiment; surgical access to the right hemitórax was assured by controiled mechanical anesthesia. The thoracotomy in the equine was further evaluated by means of a complete clinicai examination, blood gás analysis, complete blood count and thoracic ultrasonography.The suture technique aiso revealed itself efficient and the animais exbited no sings of respiratory distress, pneumotórax or thoracic wall suture dehiscense. The complete

  6. Preparation of Blood-Deficient Model and Research of Angelica Polysaccharide on Enriching Blood in Chickens

    OpenAIRE

    Haifeng Hou; Yongzhan Bao; Qian Li; Wanyu Shi

    2012-01-01

    In this study cyclophosphamide was used to prepare the blood-deficient model. The red blood cell count and hemoglobin content were measured. The experimental chickens presented the symptoms of blood-deficient syndrome, dullness, shrinkinginto oneself, broken winded, loose feather, waxy eyelid, and pale tongue. At the same time, red blood cell count and hemoglobin content decreased significantly. Angelica polysaccharide as the effective component of Angelica Sinensis could significantly increa...

  7. EFFECT OF GRADE -III EXERCISE ON TOTAL LEUCOCYTE COUNT AND DIFFERENTIAL LEUCOCYTE COUNT

    Directory of Open Access Journals (Sweden)

    Amrutha.K,

    2014-08-01

    Full Text Available Introduction: Exercise is a type of physical activity and is defined as a planned. Structured and repetitive bodily movement done to improve and to maintain physical fitness .exercise stress releases some amount of adrenaline from adrenal medulla. Adrenaline brings about a beta receptor mediated vasodilatation in skeletal muscles which may contribute to the anticipatory vasodilatation in the blood vassals, and increased blood flow is seen during and for a short while after the exercise. Due to the stimulation of bone marrow more number of white blood cells (WBC produced from marginal pool and released into circulation. Materials & Methods: Thirty subjects both male and female were randomly selected as control and study groups. Control group sample was collected after taking rest for 1 hour and study group sample was collected immediately after grade III exercise. Conclusion: After exercise total leukocyte count (TLC increased significantly p. value being ˂0.0001 and differential leukocyte count(DLC increased significantly p. value being ˂0.01.

  8. High-speed counting and sizing of cells in an impedance flow microcytometer with compact electronic instrumentation

    DEFF Research Database (Denmark)

    Castillo-Fernandez, Oscar; Rodriguez-Trujíllo, Romén; Gomila, Gabriel;

    2014-01-01

    Here we describe a high-throughput impedance flow cytometer on a chip. This device was built using compact and inexpensive electronic instrumentation. The system was used to count and size a mixed cell sample containing red blood cells and white blood cells. It demonstrated a counting capacity of...

  9. Importância clínica da dosagem de lactato e contagem de eritroblastos no sangue colhido no segmento placentário da veia umbilical para o diagnóstico de hipóxia-isquemia em prematuros Clinical value of lactate measurement and nucleated red blood cell counts in the placental segment of the umbilical vein of premature newborns for diagnosis of hypoxia-ischemia

    Directory of Open Access Journals (Sweden)

    Yvi Gea

    2007-04-01

    Full Text Available OBJETIVO: Determinar a validade clínica das dosagens de lactato e contagem de eritroblastos quando comparados com o excesso de bases (EB em sangue do segmento placentário da veia umbilical de prematuros. MÉTODOS: foram colhidas amostras de 25 prematuros, após ligadura e dequitação. Os prematuros foram seguidos até a alta. Estatística incluiu regressão linear, correlação de Spearman, curvas ROC, Teste de Fisher. RESULTADOS: Lactato mostrou boa correlação com pH e EB (p OBJECTIVE: To evaluate the clinical value of lactate measurement and nucleated red blood cell (NRBC counts when compared to base excess (BE in the blood collected from the placental segment of the umbilical vein. METHODS: 25 umbilical cords from premature babies were sampled after placental delivery and cord clamping. Babies were followed until discharge. Statistics involved linear regression, Spearman's correlation, ROC curves, and Fisher's exact test. RESULTS: The relationship between lactate in the umbilical vein blood and pH and BE was significant (p < 0.0001. A 4.04 mmol/L lactate level showed a sensitivity of 62.5% and a specificity of 94.1% in detecting pH <7.2 and BE < -10 mmol/L. NRBC counts were related to BE (p = 0.0095, but with a sensitivity of 37.5% and specificity of 82.4% in detecting BE < -10 mmol/L. CONCLUSIONS: Lactate is a valuable marker of fetal hypoxia when sampled from placental segment veins. NRBC counts demonstrated low sensitivity for the detection of acidosis.

  10. Erythrocyte Fragment Count Predicts Hemolysis in Roller Pumps

    Institute of Scientific and Technical Information of China (English)

    FAN Jun-qiang; XU Shi-wei; CHEN Fang; DING Min-jun

    2007-01-01

    Hemolysis in blood pumps has been measured by various in vitro test methods, in which normalized index of hemolysis (NIH) was established. As NIH is complicated and difficult to calculate, erythrocyte fragment count is proposed in the present study to predict hemolysis in roller pumps. Methods: Five paired in vitro tests wereconducted using the POLYSTAN pediatric pump(group A) and COBE pump(group B). Ten whole blood samples (400 ml ) were circled in the roller pump for 16 h. Erythrocyte fragments count and plasma-free hemoglobin (FHb) were measured before pumping and every two hours through circulation after four-hour-pumping. The morphological changes of erythrocyte were observed by scanning electron microscope. Results: The two groups' EFC and FHb levels were increased linearly during a long duration of pumping and linear regression of erythrocyte fragments count and plasma-free hemoglobin were correlated. Conclusion: Erythrocyte fragments count could be used as an index in evaluating the in vitro hemolytic properties of blood pumps.

  11. Star Counts and Galactic Structure

    Institute of Scientific and Technical Information of China (English)

    DU Cui-Hua; ZHOU Xu; MA Jun; CHEN Jian-Sheng

    2004-01-01

    @@ We use a model of the Galactic stellar distribution to analyse the BATC star count data toward two high Galactic latitude fields. Since star counts at high Galactic latitudes are not strongly related to the radial distribution,they are very suitable for the study of the vertical distribution of the Galaxy. The vertical density distribution of the stars shows the contribution of the thin disc, the thick disc and the stellar halo of the Galaxy. We give quantitative descriptions of these components in terms of exponential discs and a de Vaucouleurs halo. We find that the observed counts support an axial ratio of c/a ~ 0.5, implying a more flattened halo. We consider that it is possible that the halo has two subpopulations, i.e. a flattened inner halo and a spherical outer halo in the Milky Way.

  12. Sampling site matters when counting lymphocyte subpopulations.

    Directory of Open Access Journals (Sweden)

    Benson Ogunjimi

    Full Text Available Clinical and scientific work routinely relies on antecubital venipunctures for hematological, immunological or other analyses on blood. This study tested the hypothesis that antecubital veins can be considered to be a good proxy for other sampling sites. Using a hematocytometer and a flow cytometer, we analyzed the cell counts from samples coming from the radial artery, the dorsal hand veins and the antecubital veins from 18 volunteers. Most surprisingly, we identified the greatest difference not to exist between arterial and venous circulation, but between the distal (radial artery & dorsal hand veins and proximal (antecubital veins sampling sites. Naïve T cells had a higher cell count distally compared to proximally and the reverse was true for effector memory T cells. Despite these differences there were high correlations between the different sampling sites, which partially supports our initial hypothesis. Our findings are crucial for the future design and interpretation of immunological research, and for clinical practice. Furthermore, our results suggest a role for interval lymph nodes in the trafficking of lymphocytes.

  13. Tree modules and counting polynomials

    CERN Document Server

    Kinser, Ryan

    2011-01-01

    We give a formula for counting tree modules for the quiver S_g with g loops and one vertex in terms of tree modules on its universal cover. This formula, along with work of Helleloid and Rodriguez-Villegas, is used to show that the number of d-dimensional tree modules for S_g is polynomial in g with the same degree and leading coefficient as the counting polynomial A_{S_g}(d, q) for absolutely indecomposables over F_q, evaluated at q=1.

  14. Detection of the Residual Leukocytes in Blood Components:a Method of ADAM-rWBC for Microscopic Counting%一种显微计数新方法ADAM-rWBC在成分血残留白细胞检测中的应用

    Institute of Scientific and Technical Information of China (English)

    曾晓晖; 张雅丽; 洪素云; 谢蓓蓓; 曾晓新; 倪宏英; 宋秀宇; 陈长荣

    2016-01-01

    目的:评估一种基于显微计数新方法(ADAM-rWBC)在残余白细胞定量检测中的性能和应用可行性。方法以Sysmex KX21全自动血球计数法及Nageotte血细胞计数板法作为参比,分析ADAM-rWBC检测白细胞的定量准确性和可重复性,并评估其在成分血白细胞残留检测中的实际性能。结果对WBC浓度分别为40、10和1 cell/µl的3份滤白红细胞悬液样品进行平行检测,ADAM-rWBC的批间变异系数分别为8.8、11.3和31.1,准确度分别为100.0%、87.5%和37.5%;而Nageotte计数板法的批间变异系数则分别为20.4、31.8和15.3,准确度分别为50.0%、12.5%和37.5%。对36份全血标本(白细胞浓度范围为4辱与共4500~10700 cell/µl),采用ADAM-rWBC进行稀释定量与采用Sysmex kx21进行直接计数的结果间差异无统计学意义(P=0.655),且线性相关性较好(Slope=1.00±0.01, R2=0.832,P<0.001)。对34份滤白红细胞悬液进行残留白细胞测定,平行采用ADAM-rWBC和Nageotte计数法所获结果间的差异无统计学意义(P=0.18),两种方法定量值均低于3.0 cell/µl,符合白细胞残余量<12.5 cell/µl的质控要求。结论采用ADAM-rWBC进行白细胞定量测定准确可靠,相比于Nageotte计数法重复性和精度更佳,能满足成分血白细胞残余量检测的国标需求。%Objective Transfusion of blood components containing high amounts of leucocytes possibly causes side-effects in recipients. Therefore, the measurement of the residual leucocytes is certainly required for quality control of blood components. The study aimed to evaluate the performance of a new microscopic system (ADAM-rWBC) for residual leucocytes determination in blood components.Methods The quantitative accuracy, reproducibility and practical performance of ADAM-rWBC in detection of leucocytes was evaluated when compared with sysmex KX21 hematology analyzer and manual counting in the nageotte chamber

  15. Bioquímica sérica e hemograma de bovinos antes e após a técnica de biópsia hepática Serum chemistry profile and complete blood count in cattle before and after liver biopsy technique

    Directory of Open Access Journals (Sweden)

    Rogério Martins Amorim

    2003-06-01

    Full Text Available Avaliaram-se os efeitos da técnica de biópsia hepática sobre as atividades séricas das enzimas gama glutamil transferase (GGT, aspartato amino transferase (AST, fosfatase alcalina (FA, hemograma e fibrinogênio em 36 novilhas Nelore. Os animais foram submetidos ao procedimento de colheita de fragmento hepático, utilizando-se agulha de biópsia para determinação dos elementos minerais. As avaliações da bioquímica sérica foram realizadas em 20 novilhas, sendo divididas em dois grupos. No grupo 1 (n=10, colheu-se sangue em tubos a vácuo sem anticoagulante, antes da realização da biópsia e 24h após, e no grupo 2 (n=10 antes da biópsia e 96h após. Os valores do hemograma e do fibrinogênio foram obtidos antes e 96h após a realização do procedimento (n=16. A atividade sérica da AST aumentou significativamente (54,4% 24h após a realização da biópsia, porém estava dentro dos valores de referência 96h após o procedimento. As atividades séricas da GGT e da FA não sofreram aumentos nas 24h e 96h após a biópsia. A proteína total apresentou aumento significativo de 0,24g/dL 96h após o procedimento. Os demais parâmetros avaliados permaneceram dentro dos valores normais para a espécie. Os resultados obtidos permitem concluir que a técnica de biópsia hepática empregada é segura e eficaz por não provocar dano hepático significativo e por obter rapidamente fragmentos adequados para análise bromatológica e histopatológica.The effects of liver biopsy technique on GGT (gama glutamil transferase, AST (aspartate amino transferase, FA (alcaline transferase serum activity, complete blood counting and fibrinogen were evaluated in 36 Nelore breed heifers. The animals were submitted to liver biopsy, using biopsy needle for mineral element determination. The biochemical profile evaluation were obtained in 20 heifers, being divided in two groups. In group 1 (n=10, blood was obtained with vacuum tubes without anticoagulant before

  16. Repeatability of differential goat bulk milk culture and associations with somatic cell count, total bacterial count, and standard plate count

    OpenAIRE

    Koop, G.; Dik, N; Nielen, M; Lipman, L. J. A.

    2010-01-01

    The aims of this study were to assess how different bacterial groups in bulk milk are related to bulk milk somatic cell count (SCC), bulk milk total bacterial count (TBC), and bulk milk standard plate count (SPC) and to measure the repeatability of bulk milk culturing. On 53 Dutch dairy goat farms, 3 bulk milk samples were collected at intervals of 2 wk. The samples were cultured for SPC, coliform count, and staphylococcal count and for the presence of Staphylococcus aureus. Furthermore, SCC ...

  17. Verbal Counting in Bilingual Contexts

    Science.gov (United States)

    Donevska-Todorova, Ana

    2015-01-01

    Informal experiences in mathematics often include playful competitions among young children in counting numbers in as many as possible different languages. Can these enjoyable experiences result with excellence in the formal processes of education? This article discusses connections between mathematical achievements and natural languages within…

  18. Shakespeare Live! and Character Counts.

    Science.gov (United States)

    Brookshire, Cathy A.

    This paper discusses a live production of Shakespeare's "Macbeth" (in full costume but with no sets) for all public middle school and high school students in Harrisonburg and Rockingham, Virginia. The paper states that the "Character Counts" issues that are covered in the play are: decision making, responsibility and…

  19. On Counting the Rational Numbers

    Science.gov (United States)

    Almada, Carlos

    2010-01-01

    In this study, we show how to construct a function from the set N of natural numbers that explicitly counts the set Q[superscript +] of all positive rational numbers using a very intuitive approach. The function has the appeal of Cantor's function and it has the advantage that any high school student can understand the main idea at a glance…

  20. KIDS COUNT New Hampshire, 2000.

    Science.gov (United States)

    Shemitz, Elllen, Ed.

    This Kids Count report presents statewide trends in the well-being of New Hampshire's children. The statistical report is based on 22 indicators of child well-being in 5 interrelated areas: (1) children and families (including child population, births, children living with single parent, and children experiencing parental divorce); (2) economic…

  1. Counting problems for number rings

    NARCIS (Netherlands)

    Brakenhoff, Johannes Franciscus

    2009-01-01

    In this thesis we look at three counting problems connected to orders in number fields. First we study the probability that for a random polynomial f in Z[X] the ring Z[X]/f is the maximal order in Q[X]/f. Connected to this is the probability that a random polynomial has a squarefree discriminant. T

  2. Counting a Culture of Mealworms

    Science.gov (United States)

    Ashbrook, Peggy

    2007-01-01

    Math is not the only topic that will be discussed when young children are asked to care for and count "mealworms," a type of insect larvae (just as caterpillars are the babies of butterflies, these larvae are babies of beetles). The following activity can take place over two months as the beetles undergo metamorphosis from larvae to adults. As the…

  3. Teaching Emotionally Disturbed Students to Count Feelings.

    Science.gov (United States)

    Bartels, Cynthia S.; Calkin, Abigail B.

    The paper describes a program to teach high school students with emotional and behavior problems to count their feelings, thereby improving their self concept. To aid in instruction, a hierarchy was developed which involved four phases: counting tasks completed and tasks not completed, counting independent actions in class, counting perceptions of…

  4. Haemoglobin and red cell counts in leptospirosis patients infected with different serovars

    Directory of Open Access Journals (Sweden)

    Scott Benjamin Craig

    2013-04-01

    Full Text Available Introduction The aim of the study was to compare haemoglobin and red cell counts between patients known to be infected with a range of leptospiral serovars. Methods The study retrospectively compared the haemoglobin and red cell count results from the first blood samples taken from 207 patients at presentation to a Queensland Health hospital. Results Significant differences were observed in haemoglobin and red cell counts in those infected with Leptospira interrogans serovars Szwajizak and Canicola when compared with most of the other serovars. Conclusions These findings suggest that haemoglobin and red cell counts may be useful in differentiating leptospiral serovars in leptospirosis patients.

  5. Predictive Model Assessment for Count Data

    Science.gov (United States)

    2007-09-05

    critique count regression models for patent data, and assess the predictive performance of Bayesian age-period-cohort models for larynx cancer counts...the predictive performance of Bayesian age-period-cohort models for larynx cancer counts in Germany. We consider a recent suggestion by Baker and...Figure 5. Boxplots for various scores for patent data count regressions. 11 Table 1 Four predictive models for larynx cancer counts in Germany, 1998–2002

  6. INTRAOPERATIVE PREDONATION CONTRIBUTES TO BLOOD SAVING

    NARCIS (Netherlands)

    SCHONBERGER, JPAM; BREDEE, JJ; TJIAN, D; EVERTS, PAM; WILDEVUUR, CRH

    1993-01-01

    The merits of reinfusing prebypass-removed autologous blood (intraoperative predonation) to salvage blood and improve postoperative hemostasis are still debated, specifically for patients at a higher risk for bleeding. To evaluate the effect of intraoperative predonation on the platelet count, blood

  7. Importance of the TIMI frame count: implications for future trials

    OpenAIRE

    Chen Michael; Michaels Andrew D; Appleby Mark A; Gibson, Michael C.

    2000-01-01

    Abstract Although the TIMI (Thrombolysis In Myocardial Infarction) flow grade classification scheme is widely used to assess angiographic outcomes, it is limited by poor reproducibility and its categoric nature. The corrected TIMI frame count (CTFC) is a simple, more objective continuous variable index of coronary blood flow that can be broadly and inexpensively applied. This measure of the time for dye to traverse a coronary artery is both accurate (highly correlated with Doppler velocity me...

  8. The detection of peripheral blood IL-13,IL-4 and eosinophil cells count in atopic dermatitis patients%过敏性皮炎患者外周血IL-13、IL-4和嗜酸性粒细胞数检测

    Institute of Scientific and Technical Information of China (English)

    曲松本; 宋松山; 阎丽平; 牟晓峰

    2013-01-01

    Objective:To investigate the correlation of PBEC, IL-4, IL-13 and atopic dermatitis. Methods:The counting of peripheral blood eosinophil cells (PBEC) was performed by the routine method,and the level of serum IL-4 and IL-13 in 28 atopic dermatitis patients and 40 health adults was measured by ELISA.Results:The amount of PBEC ,the level of serum IL-4 and IL-13 in atopic dermatitis group was significantly higher than that in the health control (P<0.01) , and there was a positive correlation between IL-4 and IL-13 in atopic dermatitis group(P<0.05). Conclusions:There is an important role of PBEC, IL-4 and IL-13 in atopic dermatitis, and IL-13 IL-13 May play a role by IL-4.%目的:探讨外周血中嗜酸性粒细胞(peripheral blood eosinophil ce11, PBEC)计数、人白介素-13(interleukin-13,IL-13)和人白介素-4(interleukin-4,IL-4)水平的表达在过敏性皮炎发生发展中的作用。方法:收集28例过敏性皮炎患者和40例健康查体者外周血标本,常规计数PBEC,并通过半定量酶联免疫吸附试验(ELISA)检测上述标本中的IL-13和IL-4的水平,采用t检验比较其间的差异。结果:过敏性皮炎患者外周血PBEC计数、IL-13和IL-4的水平显著高于健康对照组(P<0.01),且过敏性皮炎组IL-13和IL-4水平呈显著正相关性(P<0.05)。结论:PBEC、IL-13和IL-4参与过敏性皮炎患者体内的变态反应,IL-13可能通过IL-4来发挥作用。

  9. Accurate platelet counting in an insidious case of pseudothrombocytopenia.

    Science.gov (United States)

    Lombarts, A J; Zijlstra, J J; Peters, R H; Thomasson, C G; Franck, P F

    1999-01-01

    Anticoagulant-induced aggregation of platelets leads to pseudothrombocytopenia. Blood cell counters generally trigger alarms to alert the user. We describe an insidious case of pseudothrombocytopenia, where the complete absence of Coulter counter alarms both in ethylenediaminetetraacetic acid blood and in citrate or acid citrate dextrose blood samples was compounded by the fact that the massive aggregates were exclusively found at the edges of the blood smear. Non-recognition of pseudothrombocytopenia can have serious diagnostic and therapeutic consequences. While the anti-aggregant mixture citrate-theophylline-adenosine-dipyridamole completely failed in preventing pseudothrombocytopenia, addition of iloprost to anticoagulants only partially prevented the aggregation. Only the prior addition of gentamicin to any anticoagulant used resulted in a complete prevention of pseudothrombocytopenia and enabled to count accurately the platelets.

  10. The Mayan Long Count Calendar

    CERN Document Server

    Chanier, Thomas

    2013-01-01

    The Maya had a very elaborate and accurate calendar. First, the Mayan Long Count Calendar (LCC) was used to point historical events from a selected "beginning of time". It is also characterized by the existence of a religious month Tzolk'in of 260 days and a civic year Haab' of 365 days. The LCC is supposed to begin on 11 August -3114 BC known as the Goodman-Martinez-Thompson (GMT) correlation to the Gregorian calendar based on historical facts and end on 21 December 2012 corresponding to a period of approximately 5125 years or 13 Baktun. We propose here to explain the origin the 13 Baktun cycle, the Long Count Periods and the religious month Tzolk'in.

  11. Reproducibility of Manual Platelet Estimation Following Automated Low Platelet Counts

    Directory of Open Access Journals (Sweden)

    Zainab S Al-Hosni

    2016-11-01

    Full Text Available Objectives: Manual platelet estimation is one of the methods used when automated platelet estimates are very low. However, the reproducibility of manual platelet estimation has not been adequately studied. We sought to assess the reproducibility of manual platelet estimation following automated low platelet counts and to evaluate the impact of the level of experience of the person counting on the reproducibility of manual platelet estimates. Methods: In this cross-sectional study, peripheral blood films of patients with platelet counts less than 100 × 109/L were retrieved and given to four raters to perform manual platelet estimation independently using a predefined method (average of platelet counts in 10 fields using 100× objective multiplied by 20. Data were analyzed using intraclass correlation coefficient (ICC as a method of reproducibility assessment. Results: The ICC across the four raters was 0.840, indicating excellent agreement. The median difference of the two most experienced raters was 0 (range: -64 to 78. The level of platelet estimate by the least-experienced rater predicted the disagreement (p = 0.037. When assessing the difference between pairs of raters, there was no significant difference in the ICC (p = 0.420. Conclusions: The agreement between different raters using manual platelet estimation was excellent. Further confirmation is necessary, with a prospective study using a gold standard method of platelet counts.

  12. Counting Irreducible Double Occurrence Words

    CERN Document Server

    Burns, Jonathan

    2011-01-01

    A double occurrence word $w$ over a finite alphabet $\\Sigma$ is a word in which each alphabet letter appears exactly twice. Such words arise naturally in the study of topology, graph theory, and combinatorics. Recently, double occurrence words have been used for studying DNA recombination events. We develop formulas for counting and enumerating several elementary classes of double occurrence words such as palindromic, irreducible, and strongly-irreducible words.

  13. Monitoring Milk Somatic Cell Counts

    Directory of Open Access Journals (Sweden)

    Gheorghe Şteţca

    2014-11-01

    Full Text Available The presence of somatic cells in milk is a widely disputed issue in milk production sector. The somatic cell counts in raw milk are a marker for the specific cow diseases such as mastitis or swollen udder. The high level of somatic cells causes physical and chemical changes to milk composition and nutritional value, and as well to milk products. Also, the mastitic milk is not proper for human consumption due to its contribution to spreading of certain diseases and food poisoning. According to these effects, EU Regulations established the maximum threshold of admitted somatic cells in raw milk to 400000 cells / mL starting with 2014. The purpose of this study was carried out in order to examine the raw milk samples provided from small farms, industrial type farms and milk processing units. There are several ways to count somatic cells in milk but the reference accepted method is the microscopic method described by the SR EN ISO 13366-1/2008. Generally samples registered values in accordance with the admissible limit. By periodical monitoring of the somatic cell count, certain technological process issues are being avoided and consumer’s health ensured.

  14. Approximate Counting of Graphical Realizations.

    Science.gov (United States)

    Erdős, Péter L; Kiss, Sándor Z; Miklós, István; Soukup, Lajos

    2015-01-01

    In 1999 Kannan, Tetali and Vempala proposed a MCMC method to uniformly sample all possible realizations of a given graphical degree sequence and conjectured its rapidly mixing nature. Recently their conjecture was proved affirmative for regular graphs (by Cooper, Dyer and Greenhill, 2007), for regular directed graphs (by Greenhill, 2011) and for half-regular bipartite graphs (by Miklós, Erdős and Soukup, 2013). Several heuristics on counting the number of possible realizations exist (via sampling processes), and while they work well in practice, so far no approximation guarantees exist for such an approach. This paper is the first to develop a method for counting realizations with provable approximation guarantee. In fact, we solve a slightly more general problem; besides the graphical degree sequence a small set of forbidden edges is also given. We show that for the general problem (which contains the Greenhill problem and the Miklós, Erdős and Soukup problem as special cases) the derived MCMC process is rapidly mixing. Further, we show that this new problem is self-reducible therefore it provides a fully polynomial randomized approximation scheme (a.k.a. FPRAS) for counting of all realizations.

  15. Approximate Counting of Graphical Realizations.

    Directory of Open Access Journals (Sweden)

    Péter L Erdős

    Full Text Available In 1999 Kannan, Tetali and Vempala proposed a MCMC method to uniformly sample all possible realizations of a given graphical degree sequence and conjectured its rapidly mixing nature. Recently their conjecture was proved affirmative for regular graphs (by Cooper, Dyer and Greenhill, 2007, for regular directed graphs (by Greenhill, 2011 and for half-regular bipartite graphs (by Miklós, Erdős and Soukup, 2013. Several heuristics on counting the number of possible realizations exist (via sampling processes, and while they work well in practice, so far no approximation guarantees exist for such an approach. This paper is the first to develop a method for counting realizations with provable approximation guarantee. In fact, we solve a slightly more general problem; besides the graphical degree sequence a small set of forbidden edges is also given. We show that for the general problem (which contains the Greenhill problem and the Miklós, Erdős and Soukup problem as special cases the derived MCMC process is rapidly mixing. Further, we show that this new problem is self-reducible therefore it provides a fully polynomial randomized approximation scheme (a.k.a. FPRAS for counting of all realizations.

  16. The relationship between total lymphocyte count and CD4 count in patients infected with HIV

    Directory of Open Access Journals (Sweden)

    Mokarami F

    2009-10-01

    Full Text Available "n Normal 0 false false false EN-US X-NONE AR-SA MicrosoftInternetExplorer4 /* Style Definitions */ table.MsoNormalTable {mso-style-name:"Table Normal"; mso-tstyle-rowband-size:0; mso-tstyle-colband-size:0; mso-style-noshow:yes; mso-style-priority:99; mso-style-qformat:yes; mso-style-parent:""; mso-padding-alt:0in 5.4pt 0in 5.4pt; mso-para-margin:0in; mso-para-margin-bottom:.0001pt; mso-pagination:widow-orphan; font-size:11.0pt; font-family:"Calibri","sans-serif"; mso-ascii-font-family:Calibri; mso-ascii-theme-font:minor-latin; mso-fareast-font-family:"Times New Roman"; mso-fareast-theme-font:minor-fareast; mso-hansi-font-family:Calibri; mso-hansi-theme-font:minor-latin; mso-bidi-font-family:Arial; mso-bidi-theme-font:minor-bidi;} Background: CD4 T-Lymphocyte counts have proven to be a standard laboratory marker of disease progression and severity of immunodeficiency in adults infected with HIV is used to initiate and monitor highly active antiretroviral therapy; however, its application may not be feasible for its expensive equipments and reagent in resource-limited setting. There is a need to have another marker of immunodeficiency that is less resource-demanding. In April 2002, the World Health Organization (WHO recommended that, when CD4 cell count is not available, a TLC of 1200cell/mm3 or less in individuals with stage 2 or 3 of the disease may be used as an indication to initiate ART."n"nMethods: The aim of this study was to determine the relationship between total lymphocyte count and CD4 count in HIV-infected adults. This was a retrospective cross-sectional study. Subject characteristics were patients who had positive serologic HIV test results, confirmed via western blot. Analysis unit was the results of CBC and CD4 measurements on the same blood sample each time. Data of 100 patients were collected. In this study, TLC accounts for the main predictor of CD4 count. The amounts of TLC which can predict CD4 less than 200cell/mm3 were

  17. Effect of Loratadine on Expression of Interleukin 5 and Eosinophil Count in Peripheral Blood and Bronchoal-veolar Lavage Fluid of Asthmatic Guinea Pigs%氯雷他定对哮喘豚鼠肺泡灌洗液及血清白细胞介素-5和嗜酸性粒细胞计数的影响

    Institute of Scientific and Technical Information of China (English)

    宋晓丹; 张明; 欧维琳; 郑伟华; 朱春江; 吉艳荣

    2011-01-01

    Objective To observe the influence of loratadine which was the second generation of H1 receptor antagonist on the expressions of interleukin 5 (IL- 5 )and eosinophil (EOS) count in bronchoalveolar lavage fluid(BALF) and peripheral blood in asthmatic guinea pigs,and explore the role of loratadine on airway high response of asthmatic guinea pigs. Methods Thirty -two guinea pigs were divided into normal control group, asthmatic group, low dose and high dose group of loratadine. After ovalbumin irritated, ovalbumin was inhaled to induce asthma breakout, meanwhile giving loratadine 2 mg · kg-1, 10 mg· kg- 1 in low dose group and high dose group. After the last inhaling ovalbumin,the airway pressure was detected by the animal respirator. Enzyme linked immunosorbent assay was used to measure the IL -5 in BALF and peri-pheral blood,meanwhile,EOS count was also detected. Results The serum IL -5 in normal control group,asthmatic group,low dose group and high dose group were(0.27 ±0.04) ng · L-1 ,(0.41 ±0.03) ng · L-1 ,(0. 38 ±0.02) ng · L-1 and (0.31 ±0.03) ng · L-1;IL-5 in BALF were (0. 10 ±0. 01 )ng · L-1 ,(0.38 ±0.04) ng · L-1 ,(0.33 ±0.05) ng · L-1 and (0.23 ±0.09) ng · L-1, respectively;the count of EOS in serum were(4.0 ±0. 6) × 108L-1, (54.6 ± 7.6) × 108L-1 , (44.5 ± 3.9) × l08L-1 and (29.0 ± 5.8) × 108L- 1 ;EOS in BALF in each group were(3.0 ±0. 5) × 108L-1 ,(33.7 ±3.9) × 108L-1 ,(27. 1 ±3.2) × l08L-1 and ( 18.6 ±3.3) × 108L-1. The differences between every 2 groups in each measurements were significant(Pa <0.05,0. 01 ). When inhaled histamine 10 mg · L-1 and 20 mg · L - 1, the differences between asthmatic group and low dose of loratadine group was not remarkable, but the difference between the rest 2 groups were prominent( Pa < 0. 05 ). Conclusions In asthmatic model, loratadine can ameliorate the airway high response, reverse the high IL-5 expressing condition in asthma,at the same time,the count of eosionphil is also

  18. Thromboxane A2 receptor antagonist SQ29548 reduces ischemic stroke-induced microglia/macrophages activation and enrichment, and ameliorates brain injury

    Science.gov (United States)

    Yan, Aijuan; Zhang, Tingting; Yang, Xiao; Shao, Jiaxiang; Fu, Ningzhen; Shen, Fanxia; Fu, Yi; Xia, Weiliang

    2016-01-01

    Thromboxane A2 receptor (TXA2R) activation is thought to be involved in thrombosis/hemostasis and inflammation responses. We have previously shown that TXA2R antagonist SQ29548 attenuates BV2 microglia activation by suppression of ERK pathway, but its effect is not tested in vivo. The present study aims to explore the role of TXA2R on microglia/macrophages activation after ischemia/reperfusion brain injury in mice. Adult male ICR mice underwent 90-min transient middle cerebral artery occlusion (tMCAO). Immediately and 24 h after reperfusion, SQ29548 was administered twice to the ipsilateral ventricle (10 μl, 2.6 μmol/ml, per dose). Cerebral infarction volume, inflammatory cytokines release and microglia/macrophages activation were measured using the cresyl violet method, quantitative polymerase chain reaction (qPCR), and immunofluorescence double staining, respectively. Expression of TXA2R was significantly increased in the ipsilateral brain tissue after ischemia/reperfusion, which was also found to co-localize with activated microglia/macrophages in the infarct area. Administration of SQ29548 inhibited microglia/macrophages activation and enrichment, including both M1 and M2 phenotypes, and attenuated ischemia-induced IL-1ß, IL-6, and TNF-α up-regulation and iNOS release. TXA2R antagonist SQ29548 inhibited ischemia-induced inflammatory response and furthermore reduced microglia/macrophages activation and ischemic/reperfusion brain injury. PMID:27775054

  19. Prostaglandin E2, thromboxane B2, and leukotriene B4 release from peritoneal macrophages by different osmotic agents in nonuremic guinea pigs.

    Science.gov (United States)

    Hain, H; Jörres, A; Kögel, B; Mahiout, A; Gahl, G M; Kessel, M

    1988-01-01

    Interleukin-1 (Il-1), prostaglandins, and leukotrienes have been identified as inflammatory parameters in the setting of peritoneal dialysis. Recently, it was postulated that chronic overstimulation of peritoneal macrophages (PM) may result in fibrosis and loss of ultrafiltration. The aim of the present study was to investigate whether alternative osmotic agents (polyglucose, amino acids, glycerol, bicarbonate/glucose, gelatine, hydroxyethyl starch) provoke greater eicosanoid release by PMs than glucose. Fifty milliliters of sterile dialysate containing different osmotic agents were injected intraperitoneally into nonuremic guinea pigs. After 4 hours of dwell time, prostaglandin E2 (PGE2), thromboxane B2 (TXB2), and leukotriene B4 (LTB4) production was analyzed in peritoneal effluents using specific radioimmunoassays (RIA) after liquid extraction. Cyclooxygenase products were generated with all osmotic agents: PGE2 concentrations ranged from 0.9 to 2.8 ng/4h, and TXB2 levels ranged from 39 to 49 ng/4h. In addition, the lipoxygenase product LTB4 was found in concentrations between 1.8 and 3.5 ng/4h. There were no significant differences in eicosanoid release among the osmotic agents. Thus, in this experimental setting, the capacity of PM to release inflammatory mediators did not correlate with the chemical composition of the dialysis solutions.

  20. Becoming a Blood Stem Cell Donor

    Medline Plus

    Full Text Available ... Queue __count__/__total__ Find out why Close Becoming a Blood Stem Cell Donor NCIcancertopics Loading... Unsubscribe from ... later? Sign in to add this video to a playlist. Sign in Share More Report Need to ...

  1. Repeatability of differential goat bulk milk culture and associations with somatic cell count, total bacterial count, and standard plate count

    NARCIS (Netherlands)

    Koop, G.; Dik, N.; Nielen, M.; Lipman, L.J.A.

    2010-01-01

    The aims of this study were to assess how different bacterial groups in bulk milk are related to bulk milk somatic cell count (SCC), bulk milk total bacterial count (TBC), and bulk milk standard plate count (SPC) and to measure the repeatability of bulk milk culturing. On 53 Dutch dairy goat farms,

  2. 2型糖尿病患者颈动脉斑块与外周血白细胞计数相关分析%Correlation between Atherosclerosis of Carotid Artery and White Blood Cell Count in the Elderly People with Type 2 Dia-betes Mellitus

    Institute of Scientific and Technical Information of China (English)

    冯尚勇; 朱妍; 张真稳; 王艳; 罗娜; 佘敦敏

    2016-01-01

    Objective:To investigate the correlation of white blood cell count(WBCC) with atherosclerosis of carotid artery in the elderly people with type 2 diabetes mellitus(T2DM ) .Methods:WBCC was measured with automated cell counters via standard techniques .Height ,weight ,blood pressure(BP) ,fasting blood glucose(FBG) ,hemoglobin A1c (HbA1c) ,total cholesterol (TC) ,triglyceride(TG) ,low-density lipoprotein cholesterol(LDL-C) ,high-density lipopro-tein cholesterol(HDL-C) and Lipoprotein(a)(Lp-a) were determined by routine laboratory methods .The retinol bind-ing protein 4(RBP-4) was measured by an enzyme-linked immunoabsorbent assay and the insulin concentration was measured by an electrochemiluminescence sandwich immunoassay .Results:Within the survey population carotid intima thickening plaque prevalence was 35 .4% and 29 .2% .Multivariate Logistic regression analysis showed that there were seven factors associated with the occurrence of carotid artery atherosclerosis and its risk descending were:high LDL-C , high HOM A-IR ,male ,high HbA1c ,high WBCC ,smoking and high RBP-4 .Conclusion:A high prevalence of athero-sclerosis of carotid artery was found in the elderly people with T2DM ,which was associated with high LDL-C ,high HOMA-IR ,male ,high HbA1c ,high WBCC ,smoking and high RBP-4 .%目的:探讨扬州地区中老年2型糖尿病人群颈动脉内中膜增厚及粥样斑块的患病率、危险因素及其与外周血白细胞计数的关系.方法:通过颈部血管超声检查颈动脉 ,测量身高、体重、血压 ,应用全自动生化分析仪测定空腹血糖(FBG)、甘油三酯(TG)、总胆固醇(TC)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)、脂蛋白a (LP-a).应用全自动血液分析仪进行外周血白细胞(WBC)测定.应用高压液相色谱法检测糖化血红蛋白(HbA1c).应用电化学发光夹心免疫测定血清胰岛素.应用酶联免疫吸附法测定血清视黄醇结合蛋白4 (RB P-4 ).结果:调

  3. ESTUDO DO LEUCOGRAMA COMO MONITOR DO 2-1 FOSFATO DISSÓDICO DE BETAMETASONA DO FOSFATO DISSÓDICO/ACETATO DE DEXAMETASONA, EM CÃES QUE RECEBERAM TRANSPLANTES COM CÓRNEAS DE SUÍNOS WHITE BLOOD COUNT AS A 2-1 BETAMETHASONE DISSODIC FOSFHATE AND DEXAMETHASONE DISSODIC FOSPHATE/ACETATE MONITOR IN SWINE CORNEA TRANSPLANTED DOGS

    Directory of Open Access Journals (Sweden)

    Larissa Franco de Araújo

    2007-09-01

    . Each animal had swine cornea implanted into the left eye. The swine cornea was obtained at Casa de Banha Caçula, a slaughterhouse, and conserved in a humid chamber during a maximum 6 hour period. Each animal received 4 mg dissodic fosphate/acetate dexamethasone IM and was instilled one drop of gentamycin sulphate/betametbasone dissodic fosphate, maintaining ocular medication 4 times/day and intramuscular medication once a week until euthanasia. Each animal had blood samples collected on day 0, before surgery, and on day 14 and 28 to analyse white blood count as a monitor of corticosteroids, imunocellular supressor drugs. From ten blood samples collected on day 0, 80% didn’t present any alterations, 10% presented leukocytosis with neutrophilia and 10% weren’t submitted to analysis. Due to the fact that three animals had been euthanized between days 14 and 21, these samples (40%, were analyzed and compared with these collected on day 0. Five animals (50% had their samples examined on day 28. On both days 14 and 28, there were 100% neutrophilia, 100% eosinophilia and 44.4% lymphocytopenia, indicating imunocellular supression.

    KEY-WORDS: Corticosteroids; xenotransplants; cornea; canine; swine.

  4. The significance and function of IFN-γ on the changes of peripheral blood platelet count during tumor-rejection induced by a low dose of melphalan in C57BL/6 mice%γ-干扰素在小剂量美法仑治愈肿瘤过程中对荷瘤小鼠外周血血小板计数的影响及其意义

    Institute of Scientific and Technical Information of China (English)

    李传刚; 舒晓宏; 韩丹; 李墨林; Michael Notter; 秦志海

    2009-01-01

    AIM: To investigate the significance and function of IFN-γ on the changes of peripheral blood platelet count during tumor-rejection induced by a low dose of melphalan in C57BL/6 mice. METHODS: Mouse tumor rejection model induced by a single dose of melphalan was used in this experiment. Different gene-type tumor-bearing mice (IFN-γ~(+/-) and IFN-γ~(-/-)), which had the same genetic background of C57BL/6, were treated intraperitoneally with melphalan (7.5 mg/kg). Tumor size was observed and recorded every one to three days in these different gene-type mice subsequently. Blood samples were obtained from orbital venous sinus on different days before and after melphalan treatment, and then complete blood counts were performed. The function of IFN-γ on the efficacy of chemotherapy and the changes of blood platelet count in IFN-γ~(+/-) and IFN-γ~(-/-) mice after melphalan treatment was analyzed. RESULTS: There was no significant difference in tumor sizes and blood platelet count between IFN-γ~(-/-) and IFN-γ~(+/-) mice (P>0.05). On the first day after melphalan (7.5 mg/kg) treatment, there were no significant changes in tumor sizes between mice in these two groups (P>0.05). Tumors shrank a little in IFN-γ~(-/-) mice and then grew gradually. Tumors relapsed in 2 w after melphalan injection in all IFN-γ~(-/-) mice, while tumor volumes decreased progressively and tumor cured at last in IFN-γ~(+/-) mice. The number of blood PLT in IFN-γ~(+/-) mice increased to (1935±378)×10~9/L 6 h after melphalan treatment, significantly higher than before (P0.05); While it sustained in normal range in IFN-γ~(-/-) mice. There was no significant difference in blood platelet count between IFN-γ~(-/-) and IFN-γ~(+/-) mice. CONCLUSION: Peripheral blood platelet count increased on the first day after melphalan treatment and tumors cured in IFN-γ~(+/-) mice; While tumors relapsed and there is no increase in blood platelet count on the first day after melphalan treatment in IFN

  5. Pharmacokinetics and effects on thromboxane B2 production following intravenous administration of flunixin meglumine to exercised thoroughbred horses.

    Science.gov (United States)

    Knych, H K; Arthur, R M; McKemie, D S; Chapman, N

    2015-08-01

    Flunixin meglumine is commonly used in horses for the treatment of musculoskeletal injuries. The current ARCI threshold recommendation is 20 ng/mL when administered at least 24 h prior to race time. In light of samples exceeding the regulatory threshold at 24 h postadministration, the primary goal of the study reported here was to update the pharmacokinetics of flunixin following intravenous administration, utilizing a highly sensitive liquid chromatography-mass spectrometry (LC-MS). An additional objective was to characterize the effects of flunixin on COX-1 and COX-2 inhibition when drug concentrations reached the recommended regulatory threshold. Sixteen exercised adult horses received a single intravenous dose of 1.1 mg/kg. Blood samples were collected up to 72 h postadministration and analyzed using LC-MS. Blood samples were collected from 8 horses for determination of TxB(2) and PGE(2) concentrations prior to and up to 96 h postflunixin administration. Mean systemic clearance, steady-state volume of distribution and terminal elimination half-life was 0.767 ± 0.098 mL/min/kg, 0.137 ± 0.12 L/kg, and 4.8 ± 1.59 h, respectively. Four of the 16 horses had serum concentrations in excess of the current ARCI recommended regulatory threshold at 24 h postadministration. TxB(2) suppression was significant for up to 24 h postadministration.

  6. CalCOFI Larvae Counts Positive Tows

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Fish larvae counts and standardized counts for eggs captured in CalCOFI icthyoplankton nets (primarily vertical [Calvet or Pairovet], oblique [bongo or ring nets],...

  7. CalCOFI Egg Counts Positive Tows

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Fish egg counts and standardized counts for eggs captured in CalCOFI icthyoplankton nets (primarily vertical [Calvet or Pairovet], oblique [bongo or ring nets], and...

  8. Alaska Steller Sea Lion Pup Count Database

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This database contains counts of Steller sea lion pups on rookeries in Alaska made between 1961 and 2015. Pup counts are conducted in late June-July. Pups are...

  9. Blood smear

    Science.gov (United States)

    ... some red blood cells shaped like spheres ( hereditary spherocytosis ) Increased breakdown of RBCs Presence of RBCs with ... normal Red blood cells, elliptocytosis Red blood cells, spherocytosis Acute lymphocytic leukemia - photomicrograph Red blood cells, multiple ...

  10. Blood culture

    Science.gov (United States)

    Culture - blood ... A blood sample is needed . The site where blood will be drawn is first cleaned with an antiseptic such ... organism from the skin getting into (contaminating) the blood sample and causing a false-positive result (see ...

  11. Blood Thinners

    Science.gov (United States)

    If you have some kinds of heart or blood vessel disease, or if you have poor blood flow to your brain, your doctor may recommend that you take a blood thinner. Blood thinners reduce the risk of heart ...

  12. Blood transfusions

    Science.gov (United States)

    ... this page: //medlineplus.gov/ency/patientinstructions/000431.htm Blood transfusions To use the sharing features on this ... several sources of blood which are described below. Blood From the Public (Volunteer Blood Donation) The most ...

  13. The changes in the blood of humans chronically exposed to low level gamma radiation

    Energy Technology Data Exchange (ETDEWEB)

    Knowlton, N.P. Jr. [Los Alamos Scientific Lab., NM (United States)

    1949-04-19

    This report describes a statistical study of the changes in the total white blood count and the absolute neutrophil and lymphocyte counts for ten individuals who had daily film badge exposure records and weekly blood counts over a seventy-seven week period.

  14. DC KIDS COUNT e-Databook Indicators

    Science.gov (United States)

    DC Action for Children, 2012

    2012-01-01

    This report presents indicators that are included in DC Action for Children's 2012 KIDS COUNT e-databook, their definitions and sources and the rationale for their selection. The indicators for DC KIDS COUNT represent a mix of traditional KIDS COUNT indicators of child well-being, such as the number of children living in poverty, and indicators of…

  15. Monte Carlo Simulation of Counting Experiments.

    Science.gov (United States)

    Ogden, Philip M.

    A computer program to perform a Monte Carlo simulation of counting experiments was written. The program was based on a mathematical derivation which started with counts in a time interval. The time interval was subdivided to form a binomial distribution with no two counts in the same subinterval. Then the number of subintervals was extended to…

  16. 妊娠初期外周血白细胞计数与妊娠糖尿病发生的相关性研究%Relationship between first-trimester white blood cell count and subsequent gestational diabetes mellitus

    Institute of Scientific and Technical Information of China (English)

    回园敕; 黎明; 平凡; 李伟; 张化冰; 许岭翎; 聂敏; 张丽红; 向红丁

    2011-01-01

    目的 探讨妊娠初期外周血白细胞(WBC)计数与随后发生的妊娠糖尿病(GDM)的相关性.方法 在妊娠初期检测孕妇的外周血WBC计数,将以后发生GDM的232例作为病例组,糖耐量正常的482名孕妇作为对照组,比较两组的WBC计数;再按WBC计数四分位,从而将研究对象分为四组,进行Logistic回归分析.结果 病例组的妊娠初期外周血WBC计数明显高于对照组(8.56×109/Lυs8.18×109/L,P<0.01).根据WBC计数四分位的结果,将研究对象分为四组,第一组WBC<7.46×109/L,第二组WBC(7.46~8.33)×109/L,第三组WBC(8.34~9.16)×109/L,第四组WBC≥9.17×109/L,相应的GDM的检出率分别为20.13%、34.61%、36.02%、39.61%.调整年龄、孕次、孕前BMI、SBP、DBP及糖尿病家族史等因素,行Logistic回归发现,相对于第一组而言,第二组、第三组及第四组发生GDM的危险分别是第一组的2.17倍(95%CI 1.25~3.77)、2.15倍(95%CI 1.23~3.76)及2.59倍(95%CI 1.49~4.50).结论 GDM患者妊娠初期WBC计数升高,慢性炎症可能参与了GDM的发生.%Objective To investigate the relationship between peripheral white blood cell (WBC) of the first trimester and the subsequent development of gestational diabetes mellitus (GDM).Methods WBC levels in first trimester were measured in 232 women who subsequently developed GDM and in 482 women who remained euglycemic throughout pregnancy.WBC median levels were compared between two groups using Wilcoxon's rank-sum test.Logistic regression was used to compute the unadjusted and multivariable-adjusted odds ratios for developing GDM among WBC quartile grouping.Results First trimester WBC levels were significantly increased among women who subsequently developed GDM as compared with control subjects (8.56 × 10 9 /L vs 8.18 × 10 9 /L,P < 0.01).The minimum-maximum values of white blood cell count for the first to the fourth quartile were [(2.94~7.46) × 109/L], [(7.47~8.33) × 109/L], [(8.34~9.16) × 109/L

  17. 新疆乌鲁木齐市28家不同级别医院间全血细胞计数比对调查分析%Comparative analysis of complete blood count among 28 hospitals with different grade in Urumchi City

    Institute of Scientific and Technical Information of China (English)

    李辉; 杨志伟; 张朝霞

    2008-01-01

    Objective To investigate the difference in complete blood count (CBC) within the defined time among 28 hospitals with different grade in Urumchi City, explore its influencing factors,offer the countermeasures, and further to provide scientific evidence for the mutual recognition among different medical institutions. Methods Totally 28 laboratories with different grade were enrolled in the investigation. Three fresh blood samples were employed to perform complete blood counting, in-cluding normal and pathological specimens. The measuring time and turn around time (TAT) were de-fined. Results According to the literature report on biological variability of testing results, the per-missible deviation of CBC was tentatively prescribed as follows: WBC 15% ;RBC 6% ;HB 7% ;HCT 6 % ;PLT 25 %. Qualification decision was based on the external quality assessment (EQA) criteria of Clinical Laboratory Centre of the Health Ministry. Qualification: the total score of five measured items is higher than or equal to 80%;disqualification: the total score of five measured items is lower than 80%. For the first and second sample, 26 laboratories (92.86%) were qualified;for the third sample (only 21 laboratories enrolled), 14 laboratories (67%) were qualified. Conclusion 28 laboratories par-ticipated in this test. The results of five items are congruent for the first and second sample (normalspecimens) in 28 laboratories, and the measured results are comparable. By contrast, the comparative result is unsatisfactory for the third sample (pathological specimen), mainly due to the narrow linear range of equipment. Meanwhile, the laboratory quality management should be strengthened.%目的 于新疆乌鲁木齐市不同级别医院,在规定时间内测定新鲜血液,求其测值的差异,探讨其影响因素,提出解决办法,为各医疗机构检查结果的互认提供科学依据.方法 共调查28家不同级别实验室,进行全血细胞计数(CBC),共3个标本,分正常与病理

  18. Digital coincidence counting - initial results

    Science.gov (United States)

    Butcher, K. S. A.; Watt, G. C.; Alexiev, D.; van der Gaast, H.; Davies, J.; Mo, Li; Wyllie, H. A.; Keightley, J. D.; Smith, D.; Woods, M. J.

    2000-08-01

    Digital Coincidence Counting (DCC) is a new technique in radiation metrology, based on the older method of analogue coincidence counting. It has been developed by the Australian Nuclear Science and Technology Organisation (ANSTO), in collaboration with the National Physical Laboratory (NPL) of the United Kingdom, as a faster more reliable means of determining the activity of ionising radiation samples. The technique employs a dual channel analogue-to-digital converter acquisition system for collecting pulse information from a 4π beta detector and an NaI(Tl) gamma detector. The digitised pulse information is stored on a high-speed hard disk and timing information for both channels is also stored. The data may subsequently be recalled and analysed using software-based algorithms. In this letter we describe some recent results obtained with the new acquistion hardware being tested at ANSTO. The system is fully operational and is now in routine use. Results for 60Co and 22Na radiation activity calibrations are presented, initial results with 153Sm are also briefly mentioned.

  19. The Clinical Value of Procalcitonin(PCT)and White Blood Cell(WBC)Count in Impetigo Neontorum%降钙素原(PCT)与白细胞计数联合测定在新生儿脓疱疮中的临床应用价值

    Institute of Scientific and Technical Information of China (English)

    刘晶

    2014-01-01

    目的:探讨降钙素原(PCT)与白细胞计数联合测定在新生儿脓疱疮中的临床应用价值。方法对新生儿脓疱疮组,其中包括12例新生儿脓疱疮并败血症(A组)、60例单纯新生儿脓疱疮(B组)进行PCT与WBC联合测定。结果新生儿脓疱疮组PCT与白细胞计数水平与非感染组比较有显著的统计学意义(P<0.01),新生儿脓疱疮并败血症组(A组)PCT与白细胞计数水平与新生儿脓疱疮组(B组)比较有显著的统计学意义(P<0.05)。结论PCT与白细胞计数联合测定可协助诊断新生儿脓疱疮;PCT与白细胞计数明星增高,可作为新生儿脓疱疮并败血症早期诊断有价值的指标。%Objective To delect the clinical value of procalcitonin(PCT) and white blood cell (WBC) in impetigo neontorum. Methods Combined determination of PCT and WBC count to 72case of impetigo neontorum. Results PCT and WBC levels in neonatal pustular sore group(A+B group)and the infection group(C group)compered with signiifcant statistical signiifcance(P<0.01), impetigo neonatorum with sepsis(A group) and neonatal pustular group(group B) compared with signiifcant statistical signiifcance(P<0.01).Conclusion Combined detection of PCT and WBC levels can assist the clinical neonatal pustule. It is also become as a newborn pustular sore merger sepsis early diagnosis of valuable indicators.

  20. Comparison of venous and capillary differential leukocyte counts using a standard hematology analyzer and a novel microfluidic impedance cytometer.

    Science.gov (United States)

    Hollis, Veronica S; Holloway, Judith A; Harris, Scott; Spencer, Daniel; van Berkel, Cees; Morgan, Hywel

    2012-01-01

    Capillary blood sampling has been identified as a potentially suitable technique for use in diagnostic testing of the full blood count (FBC) at the point-of-care (POC), for which a recent need has been highlighted. In this study we assess the accuracy of capillary blood counts and evaluate the potential of a miniaturized cytometer developed for POC testing. Differential leukocyte counts in the normal clinical range from fingerprick (capillary) and venous blood samples were measured and compared using a standard hematology analyzer. The accuracy of our novel microfluidic impedance cytometer (MIC) was then tested by comparing same-site measurements to those obtained with the standard analyzer. The concordance between measurements of fingerprick and venous blood samples using the standard hematology analyzer was high, with no clinically relevant differences observed between the mean differential leukocyte counts. Concordance data between the MIC and the standard analyzer on same-site measurements presented significantly lower leukocyte counts determined by the MIC. This systematic undercount was consistent across the measured (normal) concentration range, suggesting that an internal correction factor could be applied. Differential leukocyte counts obtained from fingerprick samples accurately reflect those from venous blood, which confirms the potential of capillary blood sampling for POC testing of the FBC. Furthermore, the MIC device demonstrated here presents a realistic technology for the future development of FBC and related tests for use at the site of patient care.

  1. Automatic cell counting with ImageJ.

    Science.gov (United States)

    Grishagin, Ivan V

    2015-03-15

    Cell counting is an important routine procedure. However, to date there is no comprehensive, easy to use, and inexpensive solution for routine cell counting, and this procedure usually needs to be performed manually. Here, we report a complete solution for automatic cell counting in which a conventional light microscope is equipped with a web camera to obtain images of a suspension of mammalian cells in a hemocytometer assembly. Based on the ImageJ toolbox, we devised two algorithms to automatically count these cells. This approach is approximately 10 times faster and yields more reliable and consistent results compared with manual counting.

  2. Serum inhibin-b in fertile men is strongly correlated with low but not high sperm counts: a coordinated study of 1,797 European and US men

    DEFF Research Database (Denmark)

    Jørgensen, Niels; Liu, Fan; Andersson, Anna-Maria;

    2010-01-01

    To describe associations between serum inhibin-b and sperm counts, adjusted for effect of time of blood sampling, in larger cohorts than have been previously reported.......To describe associations between serum inhibin-b and sperm counts, adjusted for effect of time of blood sampling, in larger cohorts than have been previously reported....

  3. [SEX HORMONE INFLUENCE ON PERIPHERAL NATURAL KILLER CELLS COUNT].

    Science.gov (United States)

    Ivanov, P; Konova, E; Blajeva, Sv; Lukanov, Tsv; Angelova, P; Georgieva, V; Totev, V; Komsa-Penkova, R

    2015-01-01

    Proper evaluation of immunological factors connected with pregnancy establishment increased the possibility for exact treatment in high risk gestation cases. Hormonal changes during an ovarian cycle may affect immune response, which is crucial for the embryonic implantation. Peripheral Natural killer (pNK) cells are key components of immune systems and their activities could be regulated by sex hormones. In the present study we investigated the effects of estrogen fluctuation on the number of NK cells in vivo during the early follicular and middle luteal phase of menstrual cycle. In 63 healthy women with at least one full term pregnancy and regular menstrual cycle with duration between 24 and 32 days, blood samples have been collected twice for investigation of CD3/CD16/CD56 positive lymphocytes. The mean pNK count in follicular phase was 11.6% with 4.7% variation. The median was 10.6%. The mean pNK count in luteal phase was 12.1% with 5.1% variation, respectively median for cell number 11.8%. The two-tailed t-test comparison did not find any statistical difference despite the slight elevation of pNK cells count in luteal phase. The insignificant variation in pNK cells count objected the suggestion to evaluate immunological status in women with adverse pregnancy outcome in specific phase of menstrual cycle.

  4. Discrete calculus methods for counting

    CERN Document Server

    Mariconda, Carlo

    2016-01-01

    This book provides an introduction to combinatorics, finite calculus, formal series, recurrences, and approximations of sums. Readers will find not only coverage of the basic elements of the subjects but also deep insights into a range of less common topics rarely considered within a single book, such as counting with occupancy constraints, a clear distinction between algebraic and analytical properties of formal power series, an introduction to discrete dynamical systems with a thorough description of Sarkovskii’s theorem, symbolic calculus, and a complete description of the Euler-Maclaurin formulas and their applications. Although several books touch on one or more of these aspects, precious few cover all of them. The authors, both pure mathematicians, have attempted to develop methods that will allow the student to formulate a given problem in a precise mathematical framework. The aim is to equip readers with a sound strategy for classifying and solving problems by pursuing a mathematically rigorous yet ...

  5. Photon counting compressive depth mapping

    CERN Document Server

    Howland, Gregory A; Ware, Matthew R; Howell, John C

    2013-01-01

    We demonstrate a compressed sensing, photon counting lidar system based on the single-pixel camera. Our technique recovers both depth and intensity maps from a single under-sampled set of incoherent, linear projections of a scene of interest at ultra-low light levels around 0.5 picowatts. Only two-dimensional reconstructions are required to image a three-dimensional scene. We demonstrate intensity imaging and depth mapping at 256 x 256 pixel transverse resolution with acquisition times as short as 3 seconds. We also show novelty filtering, reconstructing only the difference between two instances of a scene. Finally, we acquire 32 x 32 pixel real-time video for three-dimensional object tracking at 14 frames-per-second.

  6. Generalized Entropy Concentration for Counts

    CERN Document Server

    Oikonomou, Kostas N

    2016-01-01

    We consider the phenomenon of entropy concentration under linear constraints in a discrete setting, using the "balls and bins" paradigm, but without the assumption that the number of balls allocated to the bins is known. Therefore instead of \\ frequency vectors and ordinary entropy, we have count vectors with unknown sum, and a certain generalized entropy. We show that if the constraints bound the allowable sums, this suffices for concentration to occur even in this setting. The concentration can be either in terms of deviation from the maximum generalized entropy value, or in terms of the norm of the difference from the maximum generalized entropy vector. Without any asymptotic considerations, we quantify the concentration in terms of various parameters, notably a tolerance on the constraints which ensures that they are always satisfied by an integral vector. Generalized entropy maximization is not only compatible with ordinary MaxEnt, but can also be considered an extension of it, as it allows us to address...

  7. Pre-contraction with the thromboxane-mimetic U46619 enhances P2X receptor-mediated contractions in isolated porcine splenic artery.

    Science.gov (United States)

    Roberts, R E

    2012-06-01

    We have previously demonstrated that the thromboxane-mimetic U46619 enhances α(2)-adrenoceptor-mediated contractions through increased activation of extracellular signal-regulated kinase (ERK). In this study, we determined whether U46619 also enhances P2X-mediated contractions through the same pathway. Segments of porcine splenic artery were mounted in isolated tissue baths. Tissues were pre-contracted with U46619 to 10-20% of the response to 60 mM KCl prior to addition of α,β-methylene ATP (P2X receptor agonist). The effect of inhibition of ERK activation with the mitogen-activated protein (MAP)/ERK kinase inhibitor PD98059 (50 μM), Rho kinase inhibition with Y27632 (10 μM), p38 MAP kinase with SB203580 (10 μM) or L-type calcium channels with nifedipine (1 μM) on both the direct and enhanced contractions was then determined. U46619 enhanced the contractions to α,β-methylene ATP. Although PD98059 inhibited the direct contractions to α,β-methylene ATP, it had no effect on the U46619-enhanced contractions. Similarly, Y27632 and SB203580 inhibited the direct contractions to α,β-methylene ATP, but had no effect on the enhanced contractions. Nifedipine inhibited the responses to α,β-methylene ATP in the absence and presence of U46619. This study demonstrates that pre-contraction with U46619 enhances P2X-mediated contractions in the porcine splenic artery through a mechanism independent of ERK, Rho kinase and p38 MAP kinase. Further studies are required to determine the exact mechanism involved.

  8. A novel thromboxane A2 receptor D304N variant that abrogates ligand binding in a patient with a bleeding diathesis

    Science.gov (United States)

    Mumford, Andrew D.; Dawood, Ban B.; Daly, Martina E.; Murden, Sherina L.; Williams, Michael D.; Protty, Majd B.; Spalton, Jennifer C.; Wheatley, Mark; Mundell, Stuart J.; Watson, Steve P.

    2015-01-01

    We investigated the cause of mild mucocutaneous bleeding in a 14-year-old male patient (P1). Platelet aggregation and ATP secretion induced by arachidonic acid and the thromboxane A2 receptor (TxA2R) agonist U46619 were reduced in P1 compared with controls, whereas the responses to other platelet agonists were retained. P1 was heterozygous for a transversion within the TBXA2R gene predictive of a D304N substitution in the TxA2R. In Chinese hamster ovary-K1 cells expressing the variant D304N TxA2R, U46619 did not increase cytosolic free Ca2+ concentration, indicating loss of receptor function. The TxA2R antagonist [3H]-SQ29548 showed an approximate 50% decrease in binding to platelets from P1 but absent binding to Chinese hamster ovary-K1 cells expressing variant D304N TxA2R. This is the second naturally occurring TxA2R variant to be associated with platelet dysfunction and the first in which loss of receptor function is associated with reduced ligand binding. D304 lies within a conserved NPXXY motif in transmembrane domain 7 of the TxA2R that is a key structural element in family A G protein-coupled receptors. Our demonstration that the D304N substitution causes clinically significant platelet dysfunction by reducing ligand binding establishes the importance of the NPXXY motif for TxA2R function in vivo. PMID:19828703

  9. KI and WU polyomaviruses and CD4+ cell counts in HIV-1-infected patients, Italy.

    Science.gov (United States)

    Babakir-Mina, Muhammed; Ciccozzi, Massimo; Farchi, Francesca; Bergallo, Massimiliano; Cavallo, Rossana; Adorno, Gaspare; Perno, Carlo Federico; Ciotti, Marco

    2010-09-01

    To investigate an association between KI and WU polyomavirus (KIPyV and WUPyV) infections and CD4+ cell counts, we tested HIV-1-positive patients and blood donors. No association was found between cell counts and virus infections in HIV-1-positive patients. Frequency of KIPyV infection was similar for both groups. WUPyV was more frequent in HIV-1-positive patients.

  10. Visual and automated differential leukocyte counts. A comparison study of three instruments.

    Science.gov (United States)

    Rosvoll, R V; Mengason, A P; Smith, L; Patel, H J; Maynard, J; Connor, F

    1979-06-01

    The authors compared referee (senior author) microscopic counts, microscopic counts by several technologists, and counts obtained with two pattern-recognition leukocyte classifiers, (1) Larc and (2) Hematrak, and a cytochemical automated method for leukocyte counting, (3) Hermalog D, using samples from (1) a random patient population, (2) a selected abnormal patient population, and (3) healthy individuals. All instruments showed good accuracy and flagged abnormal results for review. Variability in pattern-recognition counts was found to be due mainly to the distribution of the cells on prepared blood smears. The Larc classifier was found to be very sensitive to minor alterations in the cytoplasm or nucleus, and rejected a number of slides. The Hemalog D showed the greatest precision. The method of preparation of slides would be a major decision factor in selecting one pattern-recognition instrument over another.

  11. Blood Donation

    Science.gov (United States)

    Tests and Procedures Blood donation By Mayo Clinic Staff Blood donation is a voluntary procedure. You agree to have blood drawn so that it can ... have a disease that requires blood components. Blood donation makes all of this possible. There are several ...

  12. Effective estimation of correct platelet counts in pseudothrombocytopenia using an alternative anticoagulant based on magnesium salt.

    Science.gov (United States)

    Schuff-Werner, Peter; Steiner, Michael; Fenger, Sebastian; Gross, Hans-Jürgen; Bierlich, Alexa; Dreissiger, Katrin; Mannuß, Steffen; Siegert, Gabriele; Bachem, Maximilian; Kohlschein, Peter

    2013-09-01

    Pseudothrombocytopenia remains a challenge in the haematological laboratory. The pre-analytical problem that platelets tend to easily aggregate in vitro, giving rise to lower platelet counts, has been known since ethylenediamine-tetra acetic acid EDTA and automated platelet counting procedures were introduced in the haematological laboratory. Different approaches to avoid the time and temperature dependent in vitro aggregation of platelets in the presence of EDTA were tested, but none of them proved optimal for routine purposes. Patients with unexpectedly low platelet counts or flagged for suspected aggregates, were selected and smears were examined for platelet aggregates. In these cases patients were asked to consent to the drawing of an additional sample of blood anti-coagulated with a magnesium additive. Magnesium was used in the beginning of the last century as anticoagulant for microscopic platelet counts. Using this approach, we documented 44 patients with pseudothrombocytopenia. In all cases, platelet counts were markedly higher in samples anti-coagulated with the magnesium containing anticoagulant when compared to EDTA-anticoagulated blood samples. We conclude that in patients with known or suspected pseudothrombocytopenia the magnesium-anticoagulant blood samples may be recommended for platelet counting.

  13. The absolute lymphocyte count accurately estimates CD4 counts in HIV-infected adults with virologic suppression and immune reconstitution

    Directory of Open Access Journals (Sweden)

    Barnaby Young

    2014-11-01

    Full Text Available Introduction: The clinical value of monitoring CD4 counts in immune reconstituted, virologically suppressed HIV-infected patients is limited. We investigated if absolute lymphocyte counts (ALC from an automated blood counting machine could accurately estimate CD4 counts. Materials and Methods: CD4 counts, ALC and HIV viral load (VL were extracted from an electronic laboratory database for all patients in HIV care at the Communicable Diseases Centre, Tan Tock Seng Hospital, Singapore (2008–13. Virologic suppression was defined as consecutive HIV VLs 300 cells/mm3. CD4 counts were estimated using the CD4% from the first value >300 and an ALC 181–540 days later. Results: A total of 1215 periods of virologic suppression were identified from 1183 patients, with 2227 paired CD4-ALCs available for analysis. 98.3% of CD4 estimates were within 50% of the actual value. 83.3% within 25% and 40.5% within 10%. The error pattern was approximately symmetrically distributed around a mean of −6.5%, but significant peaked and with mild positive skew (kurtosis 4.45, skewness 1.07. Causes for these errors were explored. Variability between lymphocyte counts measured by ALC and flow cytometry did not follow an apparent pattern, and contributed to 32% of the total error (median absolute error 5.5%, IQR 2.6–9.3. The CD4% estimate was significantly lower than the actual value (t-test, p<0.0001. The magnitude of this difference was greater for lower values, and above 25%, there was no significant difference. Precision of the CD4 estimate was similar as baseline CD4% increased, however accuracy improved significantly: from a median 16% underestimation to 0% as baseline CD4% increased from 12 to 30. Above a CD4% baseline of 25, estimates of CD4 were within 25% of the actual value 90.2% of the time with a median 2% underestimation. A robust (bisqaure linear regression model was developed to correct for the rise in CD4% with time, when baseline was 14–24

  14. Mechanism of platelet activation induced by endocannabinoids in blood and plasma.

    Science.gov (United States)

    Brantl, S Annette; Khandoga, Anna L; Siess, Wolfgang

    2014-01-01

    Platelets play a central role in atherosclerosis and atherothrombosis, and circulating endocannabinoids might modulate platelet function. Previous studies concerning effects of anandamide (N-arachidonylethanolamide) and 2-arachidonoylglycerol (2-AG) on platelets, mainly performed on isolated cells, provided conflicting results. We therefore investigated the action of three main endocannabinoids [anandamide, 2-AG and virodhamine (arachidonoylethanolamine)] on human platelets in blood and platelet-rich plasma (PRP). 2-AG and virodhamine induced platelet aggregation in blood, and shape change, aggregation and adenosine triphosphate (ATP) secretion in PRP. The EC50 of 2-AG and virodhamine for platelet aggregation in blood was 97 and 160 µM, respectively. Lower concentrations of 2-AG (20 µM) and virodhamine (50 µM) synergistically induced aggregation with other platelet stimuli. Platelet activation induced by 2-AG and virodhamine resembled arachidonic acid (AA)-induced aggregation: shape change, the first platelet response, ATP secretion and aggregation induced by 2-AG and virodhamine were all blocked by acetylsalicylic acid (ASA) or the specific thromboxane A2 (TXA2) antagonist daltroban. In addition, platelet activation induced by 2-AG and virodhamine in blood and PRP were inhibited by JZL184, a selective inhibitor of monoacylglycerol lipase (MAGL). In contrast to 2-AG and virodhamine, anandamide, a substrate of fatty acid amidohydrolase, was inactive. Synthetic cannabinoid receptor subtype 1 (CB1) and 2 (CB2) agonists lacked stimulatory as well as inhibitory platelet activity. We conclude that 2-AG and virodhamine stimulate platelets in blood and PRP by a MAGL-triggered mechanism leading to free AA and its metabolism by platelet cyclooxygenase-1/thromboxane synthase to TXA2. CB1, CB2 or non-CB1/CB2 receptors are not involved. Our results imply that ASA and MAGL inhibitors will protect platelets from activation by high endocannabinoid levels, and that

  15. Seawater Immersion Aggravates Burn Injury Causing Severe Blood Coagulation Dysfunction

    Directory of Open Access Journals (Sweden)

    Hong Yan

    2016-01-01

    Full Text Available This study aimed to investigate the endothelial function in a canine model of burn injury combined with seawater immersion. The model of burn injury was established. The dogs were randomly divided into four groups including dogs with burn injury (B group, or burn injury combined with seawater immersion (BI group, or only immersion in seawater (I group, or control animals with no injury or immersion (C group. The circulating endothelial cell (CEC count and coagulation-fibrinolysis parameters were measured. The CEC count in B group increased at 4 h, 7 h, and 10 h after injury and then reduced, whereas it continuously increased to a greater extent in BI group (P<0.05. The von Willebrand factor (vWF activity, plasminogen activator inhibitor (PAI-1, and the ratio of thromboxane B2 (TXB2 to 6-keto-prostaglandin F1α (6-K-PGF1α in BI group had a marked increase after injury, and the tissue-type plasminogen activator (tPA in the BI group decreased. Microscope observations revealed thrombus formation in lungs of the animals in BI group, but not in C, I, or B groups. Burn injury causes endothelial dysfunction, and seawater immersion lastingly aggravates this injury, leading to a higher risk of developing thrombosis.

  16. Donating Blood

    Science.gov (United States)

    ... can't get an infection or disease from giving blood. The needles and other equipment used are sterile ... part of blood (plasma) within 72 hours after giving blood. It generally takes about 4–8 weeks to ...

  17. Relationship between white blood cells and hypertension in Chinese adults: the Cardiometabolic Risk in Chinese (CRC) study.

    Science.gov (United States)

    Sun, Yu-Ting; Gong, Ying; Zhu, Ruihua; Liu, Xuekui; Zhu, Yan; Wang, Yu; Qiu, Qinqin; Qi, Lu; Liang, Jun

    2015-01-01

    Increased blood pressure was associated with increased white blood cell count (adjusted p hypertension across white blood cell count quintiles were 1.00, 0.99 (0.89-1.09), 1.11 (1.01-1.22), 1.09 (0.99-1.20), and 1.19 (1.08-1.31) (p for trend blood cell count had an additive effect on systolic blood pressure (p for interaction = 0.047). Therefore, white blood cell count could independently predict hypertension in Chinese adults.

  18. 肥胖儿童白细胞计数与糖脂代谢及心血管危险因素的关系%Correlation between White Blood Cell Count, Glucose and Lipid Metabolism and Cardiovascular Risk Factors in Obese Children

    Institute of Scientific and Technical Information of China (English)

    高芳芳; 刘戈力; 杨箐岩; 郑荣秀; 姜丽红; 鲍鹏丽; 魏莹

    2011-01-01

    目的:探讨肥胖儿童白细胞计数(WBCC)与糖脂代谢及心血管危险因素的关系.方法:收集我院儿科内分泌门诊8~15岁肥胖儿童111例.其中男84例,女27例.将WBCC分为WBCCI,WBCC2,WBCC3和WBCC4共4层,分别比较男孩、女孩各组糖脂代谢指标.对年龄及体质量指数(BMI)进行校正后采用偏相关分析不同性别儿童WBCC 与糖脂代谢及心血管危险因素的关系,多元逐步回归分析影响WBCC水平的因素.结果:男孩WBCC,BMI、腰围(WC)及三酰甘油(TG)高于女孩,而收缩压(SBP)、舒张压(DBP)、空腹血糖(FPG)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)、总胆固醇(TC),HOMA-IR及致动脉粥样硬化指数(AI)差异无统计学意义(P>0.05).男孩及女孩的WBCC4组WC及BMI均高于WBBCI组,男孩WBCC4组TG及AI高于WBCCI组,WBCC4组的HDL-C 低于WBCCI,WBCC2组(P<0.05).男孩WBCC与BMI,WC,TG及AI呈正相关(P<0.05);女孩WBCC与BMI及WC 呈正相关(P<0.05).TG,WC,HDL-C为影响WBCC的因素.结论:WBCC与肥胖儿童中心性肥胖、血脂异常及糖代谢紊乱关系密切,早期检测WBCC水平并给予早期干预,可成为防止心血管疾病发生的简单、有效措施.%Objective: To investigate association of white blood cell count (WBCC) with glucose and lipid metabolism and cardiovascular risk factors in obese children. Methods: A total of 111 subjects, 84 boys and 27 girls, aged 8-15 years were enrolled. The subjects were divided into four quartiles according to WBCC (WBCC 1-4, from the lowest to highest WBCC) in both genders. The mean values of glucose and lipid metabolism and cardiovascular risk factors were compared in boys and girls separately for each group. Bivariate correlation analysis was used to compare the relationship between WBCC,glucose and lipid metabolism and cardiovascular risk factors. Multiple regression analysis was used to evaluate the factors affecting the level of WBCC. Results: The values of WBCC, body mass

  19. Improvement of Delayed Neutron Counting System

    Institute of Scientific and Technical Information of China (English)

    YUAN; Guo-jun; XIAO; Cai-jin; YANG; Wei; ZHANG; Gui-ying; JIN; Xiang-chun; WANG; Ping-sheng; NI; Bang-fa

    2012-01-01

    <正>A new delayed neutron counting system, which is good at qualitative and quantitative analysis of fissionable nuclide mixture, will be established at China Advanced Research Reactor (CARR). We use 3 He proportional counters to count the delayed neutrons after the samples irradiated by reactor neutrons, including U3O8-stantard, uranium ore and enriched uranium. Then, the counting efficiency and limit of this system were calculated.

  20. Counting pairs of faint galaxies

    CERN Document Server

    Woods, D; Richer, H B; Woods, David; Fahlman, Gregory G; Richer, Harvey B

    1995-01-01

    The number of close pairs of galaxies observed to faint magnitude limits, when compared to nearby samples, determines the interaction or merger rate as a function of redshift. The prevalence of mergers at intermediate redshifts is fundamental to understanding how galaxies evolve and the relative population of galaxy types. Mergers have been used to explain the excess of galaxies in faint blue counts above the numbers expected from no-evolution models. Using deep CFHT (I\\leq24) imaging of a ``blank'' field we find a pair fraction which is consistent with the galaxies in our sample being randomly distributed with no significant excess of ``physical'' close pairs. This is contrary to the pair fraction of 34\\%\\pm9\\% found by Burkey {\\it et al.} for similar magnitude limits and using an identical approach to the pair analysis. Various reasons for this discrepancy are discussed. Colors and morphologies of our close pairs are consistent with the bulk of them being random superpositions although, as indicators of int...

  1. Vector perturbations of galaxy number counts

    Science.gov (United States)

    Durrer, Ruth; Tansella, Vittorio

    2016-07-01

    We derive the contribution to relativistic galaxy number count fluctuations from vector and tensor perturbations within linear perturbation theory. Our result is consistent with the the relativistic corrections to number counts due to scalar perturbation, where the Bardeen potentials are replaced with line-of-sight projection of vector and tensor quantities. Since vector and tensor perturbations do not lead to density fluctuations the standard density term in the number counts is absent. We apply our results to vector perturbations which are induced from scalar perturbations at second order and give numerical estimates of their contributions to the power spectrum of relativistic galaxy number counts.

  2. Vector perturbations of galaxy number counts

    CERN Document Server

    Durrer, Ruth

    2016-01-01

    We derive the contribution to relativistic galaxy number count fluctuations from vector and tensor perturbations within linear perturbation theory. Our result is consistent with the the relativistic corrections to number counts due to scalar perturbation, where the Bardeen potentials are replaced with line-of-sight projection of vector and tensor quantities. Since vector and tensor perturbations do not lead to density fluctuations the standard density term in the number counts is absent. We apply our results to vector perturbations which are induced from scalar perturbations at second order and give numerical estimates of their contributions to the power spectrum of relativistic galaxy number counts.

  3. 联合检测血清降钙素原、白细胞计数对脓毒症患儿感染情况及病情程度的诊断价值%The diagnostic value of combined detection of serum levels procalcitonin and white blood cell count in children with sepsis

    Institute of Scientific and Technical Information of China (English)

    曾明; 程樱; 付琼华; 王芳; 陈建昌

    2015-01-01

    目的:探究联合检测血清降钙素原(PCT)、白细胞计数(WBC)在脓毒症患儿感染情况及病情程度诊断中的应用价值。方法选取2013年1月—2014年1月收治的符合脓毒症诊断标准的患儿80例,按照病情严重程度分为脓毒症组和严重脓毒症组,每组40例。另选取同期住院的非脓毒症患儿36例作为对照组。患者入院后24h内均检测血清中PCT、WBC、C反应蛋白(CRP)水平并进行比较。比较PCT单独检测及PCT联合WBC检测诊断脓毒症的价值。结果严重脓毒症组患儿血清中PCT、WBC、CRP含量最高,分别为(2.88±1.31)μg/L、(17.0±6.6)×09/L、(124.8±15.9)mg/L,均明显高于其他2组(F=4.881、5.052、5.456,P<0.05),且患儿血清PCT阳性率为5.0%,高于脓毒症组患儿的57.5%和对照组的2.7%,3组患儿的PCT阳性率比较差异有统计学意义(F=7.34,<0.05)。PCT、WBC联合检测诊断脓毒症的敏感度、特异度、准确率、阳性预测值、阴性预测值、约登指数分别为7.5%、95.0%、91.3%、94.6%、88.4%、0.825,均明显高于PCT单独检测的57.5%、80.0%、68.8%、74.2%、65.3%、.375(P<0.05)。结论PCT在脓毒症的诊断中具有较高的应用价值,其与WBC计数联合检测后,诊断准确率明显提高。%Objective To explore the application value of combined detection of serum procalcitonin ( PCT) ,white blood cell count (WBC) in the diagnosis of sepsis in children with sepsis .Methods From January 2013 to January 2014, ac-cording to the severity of the disease , 80 children with sepsis were divided into sepsis group and severe sepsis group with 40 cases in each group .A control group of 36 patients with non sepsis patients were selected as the control group .The serum lev-els of PCT, WBC, CRP were detected in 24 h after admission.Results Severe sepsis group

  4. Comparison of different methods of platelet count and its clinical application

    Institute of Scientific and Technical Information of China (English)

    Bin Li; Chuan Wang

    2016-01-01

    Objective:To compare the results of different platelet mean volume (MPV) with the method of instrument and manual counting of platelet and its application in clinical practice.Method:According to prompts Sysmex xn (10) B4 detection results MPV. The 246 samples divided for four groups, with Sysmex xn (10) B4, Mindray BC6800 blood cell analyzer, artificial bovine Bao counting plate platelet count number. The detection results are paired t-test.Results:When 5.4 fL≤MPV≤7.4 fL, 2 instruments and artificial platelet count results showed no significant difference; When 7.5 fL≤MCV≤9.0 fL, 2 instruments and artificial platelet count results showed no significant difference, Sysmex XN (10) B4 slightly lower test results, Mindray BC6800 test results closer to the true value; When 9.1 fL≤MCV≤12.0 fL, Sysmex XN (10) B4 and artificial platelet count results were significantly, and manual counting Mindray BC6800 platelet count was no significant difference; When MPV≧ 12.1 fL, 2 instrument testing platelet counting and artificial platelets results difference had statistical significance, platelet test results between two instruments are low.Conclusion:MPV is the main factors of effect of platelet count by apparatus, when the MPV increase should be the manual assay of platelet count in order to ensure accuracy, more realistically reflect the actual situation in the patient, and provide more valuable diagnostic basis for clinical.

  5. Arctigenin reduces blood pressure by modulation of nitric oxide synthase and NADPH oxidase expression in spontaneously hypertensive rats.

    Science.gov (United States)

    Liu, Ying; Wang, Guoyuan; Yang, Mingguang; Chen, Haining; zhao, Yan; Yang, Shucai; Sun, Changhao

    2015-12-25

    Arctigenin is a bioactive constituent from dried seeds of Arctium lappa L., which was traditionally used as medicine. Arctigenin exhibits various bioactivities, but its effects on blood pressure regulation are still not widely studied. In this study, we investigated antihypertensive effects of arctigenin by long-term treatment in spontaneously hypertensive rats (SHRs). Arctigenin (50 mg/kg) or vehicle was administered to SHRs or Wistar rats as negative control by oral gavage once a day for total 8 weeks. Nifedipine (3 mg/kg) was used as a positive drug control. After treatment, hemodynamic and physical parameters, vascular reactivity in aorta, the concentration of plasma arctigenin and serum thromboxane B2, NO release and vascular p-eNOS, p-Akt, caveolin-1 protein expression, and vascular superoxide anion generation and p47phox protein expression were detected and analyzed. The results showed that arctigenin significantly reduced systolic blood pressure and ameliorated endothelial dysfunction of SHRs. Arctigenin reduced the levels of thromboxane B2 in plasma and superoxide anion in thoracic aorta of SHRs. Furthermore, arctigenin increased the NO production by enhancing the phosphorylation of Akt and eNOS (Ser 1177), and inhibiting the expression of NADPH oxidase in thoracic aorta of SHRs. Our data suggested that antihypertensive mechanisms of arctigenin were associated with enhanced eNOS phosphorylation and decreased NADPH oxidase-mediated superoxide anion generation.

  6. Antiplatelet Effect of Catechol Is Related to Inhibition of Cyclooxygenase, Reactive Oxygen Species, ERK/p38 Signaling and Thromboxane A2 Production

    Science.gov (United States)

    Wang, Tong-Mei; Lin, Bor-Ru; Yeung, Sin-Yuet; Yeh, Chien-Yang; Cheng, Ru-Hsiu; Jeng, Jiiang-Huei

    2014-01-01

    Catechol (benzenediol) is present in plant-derived products, such as vegetables, fruits, coffee, tea, wine, areca nut and cigarette smoke. Because platelet dysfunction is a risk factor of cardiovascular diseases, including stroke, atherosclerosis and myocardial infarction, the purpose of this study was to evaluate the anti-platelet and anti-inflammatory effect of catechol and its mechanisms. The effects of catechol on cyclooxygenase (COX) activity, arachidonic acid (AA)-induced aggregation, thromboxane B2 (TXB2) production, lactate dehydrogenase (LDH) release, reactive oxygen species (ROS) production and extracellular signal-regulated kinase (ERK)/p38 phosphorylation were determined in rabbit platelets. In addition, its effect on IL-1β-induced prostaglandin E2 (PGE2) production by fibroblasts was determined. The ex vivo effect of catechol on platelet aggregation was also measured. Catechol (5-25 µM) suppressed AA-induced platelet aggregation and inhibited TXB2 production at concentrations of 0.5–5 µM; however, it showed little cytotoxicity and did not alter U46619-induced platelet aggregation. Catechol (10–50 µM) suppressed COX-1 activity by 29–44% and COX-2 activity by 29–50%. It also inhibited IL-1β-induced PGE2 production, but not COX-2 expression of fibroblasts. Moreover, catechol (1–10 µM) attenuated AA-induced ROS production in platelets and phorbol myristate acetate (PMA)-induced ROS production in human polymorphonuclear leukocytes. Exposure of platelets to catechol decreased AA-induced ERK and p38 phosphorylation. Finally, intravenous administration of catechol (2.5–5 µmole/mouse) attenuated ex vivo AA-induced platelet aggregation. These results suggest that catechol exhibited anti-platelet and anti-inflammatory effects, which were mediated by inhibition of COX, ROS and TXA2 production as well as ERK/p38 phosphorylation. The anti-platelet effect of catechol was confirmed by ex vivo analysis. Exposure to catechol may affect platelet

  7. Antiplatelet effect of catechol is related to inhibition of cyclooxygenase, reactive oxygen species, ERK/p38 signaling and thromboxane A2 production.

    Directory of Open Access Journals (Sweden)

    Mei-Chi Chang

    Full Text Available Catechol (benzenediol is present in plant-derived products, such as vegetables, fruits, coffee, tea, wine, areca nut and cigarette smoke. Because platelet dysfunction is a risk factor of cardiovascular diseases, including stroke, atherosclerosis and myocardial infarction, the purpose of this study was to evaluate the anti-platelet and anti-inflammatory effect of catechol and its mechanisms. The effects of catechol on cyclooxygenase (COX activity, arachidonic acid (AA-induced aggregation, thromboxane B2 (TXB2 production, lactate dehydrogenase (LDH release, reactive oxygen species (ROS production and extracellular signal-regulated kinase (ERK/p38 phosphorylation were determined in rabbit platelets. In addition, its effect on IL-1β-induced prostaglandin E2 (PGE2 production by fibroblasts was determined. The ex vivo effect of catechol on platelet aggregation was also measured. Catechol (5-25 µM suppressed AA-induced platelet aggregation and inhibited TXB2 production at concentrations of 0.5-5 µM; however, it showed little cytotoxicity and did not alter U46619-induced platelet aggregation. Catechol (10-50 µM suppressed COX-1 activity by 29-44% and COX-2 activity by 29-50%. It also inhibited IL-1β-induced PGE2 production, but not COX-2 expression of fibroblasts. Moreover, catechol (1-10 µM attenuated AA-induced ROS production in platelets and phorbol myristate acetate (PMA-induced ROS production in human polymorphonuclear leukocytes. Exposure of platelets to catechol decreased AA-induced ERK and p38 phosphorylation. Finally, intravenous administration of catechol (2.5-5 µmole/mouse attenuated ex vivo AA-induced platelet aggregation. These results suggest that catechol exhibited anti-platelet and anti-inflammatory effects, which were mediated by inhibition of COX, ROS and TXA2 production as well as ERK/p38 phosphorylation. The anti-platelet effect of catechol was confirmed by ex vivo analysis. Exposure to catechol may affect platelet function

  8. 血栓素A2与慢性肺部炎症性疾病%Thromboxane A2 and chronic pulmonary inflammatory disease

    Institute of Scientific and Technical Information of China (English)

    李继琼; 文富强

    2008-01-01

    在慢性阻塞性肺疾病、支气管哮喘和肺囊性纤维化等气道慢性炎症性疾病中,常伴有广泛的气道结构破坏、重塑和黏液高分泌等病理改变.炎症介质血栓素A2(TXA2)是花生四烯酸的环氧合酶代谢产物之一,因其诱导血小板聚集、收缩血管及呼吸道平滑肌和刺激气道及血管平滑肌增殖而在这些病理改变中起着重要作用.TXA2作为一种重要的炎症介质,能刺激血管收缩,进而引起气道微血管渗漏,气道黏液增多,刺激气道平滑肌收缩进而引起气道阻力增加,并参与多种肺部炎症反应,近来还发现其可能参与杯状细胞化生和黏液分泌.现就其在肺部炎性疾病的作用作一综述.%Airway destruction remodeling,and mucous hypersecretion are involved in the process of pulmonary inflammatory diseases such as chronic obstructive pulmonary disease(COPD),asthma and cystic fibrosis.Thromboxane A2(TXA2),one of the cyclooxygenase metabolites of arachidonic acid,plays an important role in the pathogenesis of pulmonary diseases,which stimulates contraction of vascullar and airway smooth muscle to result in airway micrangium leakage,mucous hypersecretion and airway resistance augmentation.TXA2,as an important inflammatory mediator,participates in various kinds of pulmonary inflammatory reactions.Recent studies have reported that TXA2 may also participate in goblet cell metaplasia and mucous hypersecretion,which are reviewed in the article.

  9. It Is Time to Count Learning Communities

    Science.gov (United States)

    Henscheid, Jean M.

    2015-01-01

    As the modern learning community movement turns 30, it is time to determine just how many, and what type, of these programs exist at America's colleges and universities. This article first offers a rationale for counting learning communities followed by a description of how disparate counts and unclear definitions hamper efforts to embed these…

  10. Is It Counting, or Is It Adding?

    Science.gov (United States)

    Eisenhardt, Sara; Fisher, Molly H.; Thomas, Jonathan; Schack, Edna O.; Tassell, Janet; Yoder, Margaret

    2014-01-01

    The Common Core State Standards for Mathematics (CCSSI 2010) expect second grade students to "fluently add and subtract within 20 using mental strategies" (2.OA.B.2). Most children begin with number word sequences and counting approximations and then develop greater skill with counting. But do all teachers really understand how this…

  11. Importance of the TIMI frame count: implications for future trials

    Directory of Open Access Journals (Sweden)

    Chen Michael

    2000-08-01

    Full Text Available Abstract Although the TIMI (Thrombolysis In Myocardial Infarction flow grade classification scheme is widely used to assess angiographic outcomes, it is limited by poor reproducibility and its categoric nature. The corrected TIMI frame count (CTFC is a simple, more objective continuous variable index of coronary blood flow that can be broadly and inexpensively applied. This measure of the time for dye to traverse a coronary artery is both accurate (highly correlated with Doppler velocity measurements and precise (reproducible. The method has been prospectively validated as providing independent risk stratification above and beyond the conventional TIMI flow grades. It has been shown to be a predictor of restenosis, and has been of value in elucidating the underlying pathophysiology of acute myocardial infarction. In view of the above and its ease of use, we anticipate that CTFC will become a widely used method to evaluate coronary blood flow.

  12. Blood Smear

    Science.gov (United States)

    ... Values can fluctuate at times of illness or stress; intense exercise or smoking can also affect cell counts. ^ Back to top ... Health Professionals Get the Mobile App iTunes | Android | Kindle ...

  13. Artificial blood

    Directory of Open Access Journals (Sweden)

    Sarkar Suman

    2008-01-01

    Full Text Available Artificial blood is a product made to act as a substitute for red blood cells. While true blood serves many different functions, artificial blood is designed for the sole purpose of transporting oxygen and carbon dioxide throughout the body. Depending on the type of artificial blood, it can be produced in different ways using synthetic production, chemical isolation, or recombinant biochemical technology. Development of the first blood substitutes dates back to the early 1600s, and the search for the ideal blood substitute continues. Various manufacturers have products in clinical trials; however, no truly safe and effective artificial blood product is currently marketed. It is anticipated that when an artificial blood product is available, it will have annual sales of over $7.6 billion in the United States alone.

  14. Artificial blood.

    Science.gov (United States)

    Sarkar, Suman

    2008-07-01

    Artificial blood is a product made to act as a substitute for red blood cells. While true blood serves many different functions, artificial blood is designed for the sole purpose of transporting oxygen and carbon dioxide throughout the body. Depending on the type of artificial blood, it can be produced in different ways using synthetic production, chemical isolation, or recombinant biochemical technology. Development of the first blood substitutes dates back to the early 1600s, and the search for the ideal blood substitute continues. Various manufacturers have products in clinical trials; however, no truly safe and effective artificial blood product is currently marketed. It is anticipated that when an artificial blood product is available, it will have annual sales of over $7.6 billion in the United States alone.

  15. Survival and function of phagocytes in blood culture media

    DEFF Research Database (Denmark)

    Fischer, T K; Prag, J; Kharazmi, A

    1999-01-01

    The survival and function of human phagocytes in sterile aerobic and anaerobic blood culture media were investigated using neutrophil morphology, white blood cell count in a haemoanalyser, flow cytometry, oxidative burst response, and bactericidal effect in Colorbact and Septi-Chek blood culture...

  16. Noun Countability; Count Nouns and Non-count Nouns, What are the Syntactic Differences Between them?

    Directory of Open Access Journals (Sweden)

    Azhar A. Alkazwini

    2016-11-01

    Full Text Available Words that function as the subjects of verbs, objects of verbs or prepositions and which can have a plural form and possessive ending are known as nouns. They are described as referring to persons, places, things, states, or qualities and might also be used as an attributive modifier. In this paper, classes and subclasses of nouns shall be presented, then, noun countability branching into count and non-count nous shall be discussed. A number of present examples illustrating differences between count and non-count nouns and this includes determiner-head-co-occurrence restrictions of number, subject-verb agreement, in addition to some exceptions to this agreement rule shall be discussed. Also, the lexically inherent number in nouns and how inherently plural nouns are classified in terms of (+/- count are illustrated. This research will discuss partitive construction of count and non-count nouns, nouns as attributive modifier and, finally, conclude with the fact that there are syntactic difference between count and non-count in the English Language. Keywords: English Language, Nouns, Count, Non-count, Syntactic Differences, Proper Nouns

  17. Thrombocyte counts in mice after the administration of methanolic extract of Melastoma malabathricum

    Institute of Scientific and Technical Information of China (English)

    Sundram Karupiah; Zhari Ismail

    2013-01-01

    Objective: To investigate the effect of methanolic extract of Melastoma malabathricum (M.malabathricum Methods: Methanolic extract of M. malabathricum corresponding to 1.5 to 2 mg/10 g body weight in saline was administered to mices via oral route. Control group was given normal saline. Twenty five microlitres of blood were drawn at 0 h and thereafter at 1, 2, 4, 8, 12, 20 h after dosing via tail bleeding technique. The thrombocytes were counted in the triple laminated middle 25 squares of the haemocytometer using light microscope.Results:) in thrombocyte counts in mice. M. malabathricum treated group shows significant rise within 2nd h in thrombocyte counts with an increment of 51.64% compared to baseline count. The control group showed a moderate rise after the administration of saline whereas the Conclusions: Based on the results, it can be concluded that M. malabathricum could be a potential remedy in treating thrombocytopenic condition.

  18. Diagnosing Septic Arthritis in the Synovial White Cell Count "Gray Zone".

    Science.gov (United States)

    Ruzbarsky, Joseph J; Gladnick, Brian P; Dodwell, Emily

    2016-07-01

    Differentiating septic arthritis of the pediatric hip from other causes of hip pain and effusion continues to present a diagnostic challenge for the clinician. Although septic arthritis traditionally has been reported to have a synovial white blood cell count of 75,000 cells/mm3 or greater, lower counts can be seen in this condition. In cases where a synovial sample has been obtained and the cell count falls in the intermediate range between 25,000 and 75,000 cells/mm(3), it is unclear what proportion of these cases may be truly septic hips. In this evidence-based review, we examine Heyworth et al's study focusing on the predictive value of this intermediate white cell count range in a Lyme-endemic region.

  19. Lymphocyte count and mortality risk in older persons. The Leiden 85-Plus Study

    NARCIS (Netherlands)

    Izaks, GJ; Remarque, EJ; Becker, SV; Westendorp, RGJ

    2003-01-01

    OBJECTIVES: To investigate whether a low peripheral blood lymphocyte count is associated with increased mortality risk in older persons and to determine whether this association could be ascribed to ill health. DESIGN: A cohort study with a total follow-up period of 1,602 person years. SETTING: Leid

  20. Peripheral T-cell count in sixty cases of cutaneous vasculitis

    Directory of Open Access Journals (Sweden)

    Mittal RR

    1996-01-01

    Full Text Available ABSTRACT: Clinically diagnosed 60 cases of cutaneous vasculitis (CV were collected from Dermato-venereology Department. T-cell count in the peripheral blood, a marker of cell-mediated immunity, was done in all the cases by Thomson Method 1977. Statistically significant increase in peripheral T-lymphocytes was seen in 15/60 (25 percent cases of CV.

  1. B Counting at BaBar

    Energy Technology Data Exchange (ETDEWEB)

    McGregor, Grant Duncan

    2008-12-16

    In this thesis we examine the method of counting B{bar B} events produced in the BABAR experiment. The original method was proposed in 2000, but improvements to track reconstruction and our understanding of the detector since that date make it appropriate to revisit the B Counting method. We propose a new set of cuts designed to minimize the sensitivity to time-varying backgrounds. We find the new method counts B{bar B} events with an associated systematic uncertainty of {+-} 0.6%.

  2. Blood Clots

    Science.gov (United States)

    ... or prevent blood clots from dissolving properly. Risk factors for excessive blood clotting include Certain genetic disorders Atherosclerosis Diabetes Atrial fibrillation Overweight, obesity, and metabolic syndrome Some medicines Smoking deep vein ...

  3. Blood Transfusion

    Science.gov (United States)

    ... to their work or home. The availability of plastic bags that can have one or more satellite bags ... in preparing the donated blood. The use of plastic bags allows the blood center to make a variety ...

  4. Acurácia diagnóstica do leucograma, proteína C-reativa, interleucina-6 e fator de necrose tumoral-alfa na sepse neonatal tardia Accuracy of white blood cell count, C-reactive protein, interleukin-6 and tumor necrosis factor alpha for diagnosing late neonatal sepsis

    Directory of Open Access Journals (Sweden)

    Jamil P. S. Caldas

    2008-12-01

    Full Text Available OBJETIVO: Avaliar o valor do leucograma, proteína C-reativa (PCR, interleucina-6 (IL-6 e do fator de necrose tumoral-alfa (TNF-α, isoladamente e em conjunto, na detecção da sepse neonatal tardia. MÉTODOS: Estudo de validação diagnóstica. A PCR, IL-6 e TNF-α foram dosados por quimioluminescência à suspeita clínica, 24 e 48 horas depois, e o leucograma unicamente à suspeita. De acordo com evolução clínica e resultados de culturas, três grupos foram definidos: sepse comprovada (SC, sepse provável (SP e não infectados (NI. Os testes estatísticos utilizados foram os de Wilcoxon, qui-quadrado e análise de variância de Friedman e os limites de corte foram obtidos pela construção da curva ROC. RESULTADOS: Estudaram-se 82 crianças, sendo 42 no grupo SC, 16 no SP e 24 NI. Nos três momentos, as medianas da PCR e da IL-6 mostraram-se significativamente mais elevadas nos grupos SC e SP, e as do TNF-α alteraram-se apenas no grupo SC. Os índices diagnósticos da PCR foram elevados nos três momentos e com acurácia superior a do leucograma e semelhante a da IL-6 e a do TNF-α em suas primeiras medidas. Entre as citocinas, não houve diferença estatística entre elas, nem em relação ao leucograma. A associação dos testes não aumentou a capacidade diagnóstica, exceto na combinação entre leucograma e PCR2 e na dosagem seriada de PCR. CONCLUSÕES: A PCR e o leucograma mostram-se úteis no diagnóstico de sepse neonatal tardia e comparáveis à IL-6 e ao TNF-α. A acurácia aumentou com a associação PCR-leucograma e a dosagem seriada da PCR.OBJECTIVE: To evaluate the diagnostic value for late neonatal sepsis of white blood cell count (WBC and assays for C-reactive protein (CRP, interleukin-6 (IL-6 and tumor necrosis factor alpha (TNF-α, in isolation and in conjunction. METHODS: This was a diagnostic test validation study. Chemiluminescence was used to assay CRP, IL-6 and TNF-α at the time of clinical suspicion and again

  5. HIV/HCV共感染者外周血辅助性T细胞17和IL-17A水平检测%Th17 cell counts in peripheral blood and serum IL-17A levels in HIV/HCV co-infected patients

    Institute of Scientific and Technical Information of China (English)

    丁佩佩; 何纲; 陈晓华; 甄沛林; 吴兴柳; 胡长征

    2015-01-01

    目的:探讨HIV/HCV共感染者外周血辅助性T细胞17(Th17)及白细胞介素(IL)-17A的免疫调节作用。方法随机选取HIV/HCV共感染者、HIV感染者、HCV感染者和健康人各30例,使用流式细胞仪检测外周血CD4+T细胞数量和Th17数量,采用ELISA法检测血清IL-17A水平。结果 HIV感染者和HIV/HCV共感染者CD4+T淋巴细胞分别为(310.23±114.35)个/μl和(218.42±112.47)个/μl,均较健康人明显降低[(735.46±121.52)个/μl,P<0.05],HIV/HCV共感染者较HIV感染者CD4+T淋巴细胞更低(P<0.05),HCV感染者CD4+T淋巴细胞数为(719.47±123.72)个/μl,与健康人比较无显著性差异;HIV感染者Th17百分比和IL-17A水平分别为(2.48±0.90)%,和(25.18±12.63)pg/ml,均较健康人显著降低[(3.95±1.23)%和(39.15±16.30)pg/ml,P<0.05],HCV感染者Th17百分比和IL-17A为(5.48±0.90)%和(45.24±15.72)pg/ml,显著高于健康人(P<0.05),而HIV/HCV共感染者Th17百分比为(1.76±0.42)%,IL-17A为(16.49±7.54)pg/mL,均显著低于HIV感染者[(2.48±0.90)%和(25.18±12.63)pg/mL,P<0.05]。结论合并HCV感染可能通过影响Th17及其细胞因子IL-17A进一步影响HIV患者的免疫功能。%Objective To explore the changes in Thl7 cell counts and serum IL-17A levels in HIV/HCV co-infected patients. Methods Thirty HIV/HCV co-infected patients,30 HIV infected patients,30 HCV infected patients and 30 healthy subjects were selected in this study. The percentage of CD4+T cells and Th17 cells in peripheral blood,and IL-17A in sera were detected by FCM and ELISA,respectively. Results The percentage of CD4+T cells in HIV and HIV/HCV co-infected patients were (310.23±114.35)/μl and(218.42±112.47)/μl,respec-tively,both were significantly lower than that in the healthy controls [(735.46±121.52)/μl,P<0.05],while the per-centage of CD4+T cells in HIV/HCV co-infected group was even lower

  6. Skin tags: A link between lesional mast cell count/tryptase expression and obesity and dyslipidemia

    Directory of Open Access Journals (Sweden)

    Samar Abdallah M Salem

    2013-01-01

    Full Text Available Background:The etiology of skin tags (STs is not fully understood. A relation to diabetes mellitus and obesity was suggested. Few studies of possible mast cells (MCs involvement were reported. Tyrptase is a mast cell mediator and a potent fibroblast growth factor. It may provide a molecular link between mast cell activation and fibrosis. Aims: The aim was to assess clinical and laboratory findings in patients with STs, and the possible link between obesity, dyslipidemia, and lesional MC count/tryptase expression. Materials and Methods: A total of 20 patients with STs were subjected to clinical examination, estimation of body mass index (BMI, fasting blood glucose (FBG, postprandial blood glucose (PPBG, serum cholesterol and triglycerides, abdominal ultrasound for fatty liver assessment, in addition to study of MCs through staining for MC tryptase in two skin biopsies; lesional and nonlesional (control. Results:All patients showed abnormally high BMI and hypertriglyceridemia, with abnormal sonographic pattern in 15 patients (75%. STs number positively correlated with the age of patients. STs showed significantly higher MC counts and tryptase expression, compared with control skin ( P < 0.001, with no correlation of the STs number or MC count with BMI, FBG, PPBG or serum cholesterol. Obese patients showed a significantly higher MC count than overweight and there was a positive correlation between MC count and serum triglycerides. Axilla and under breast STs showed a higher MC count compared with other sites. Conclusions:STs seem to be related to obesity and hypertriglyceridemia. MCs with their tryptase are possibly involved in pathogenesis of STs. MC count is related to the associated factors; obesity and serum triglycerides. MC tryptase expression is a reliable method for accurate tissue MC counting.

  7. Four square mile survey pair count instructions

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — This standard operating procedure (SOP) provides guidance for conducting bird pair count measurements on wetlands for the HAPETs Four-Square-Mile survey. This set of...

  8. Calorie count - sodas and energy drinks

    Science.gov (United States)

    ... ency/patientinstructions/000888.htm Calorie count - sodas and energy drinks To use the sharing features on this page, ... to have a few servings of soda or energy drinks a day without thinking about it. Like other ...

  9. Uranium Determination by Delayed Neutron Counting

    Institute of Scientific and Technical Information of China (English)

    2008-01-01

    <正>Uranium is a very important resource in nuclear industry, especially in the exploiture of nuclear energy. Determination of uranium using delayed neutron counting (DNC) is simple, non-destructive, and

  10. Furbearer track count index testing and development

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — Indices of abundance can be useful in monitoring furbearer populations where actual counts of individual animals are difficult. I sampled marten and snowshoe hare...

  11. CoC Housing Inventory Count Reports

    Data.gov (United States)

    Department of Housing and Urban Development — Continuum of Care (CoC) Homeless Assistance Programs Housing Inventory Count Reports are a snapshot of a CoC’s housing inventory, available at the national and state...

  12. Mourning Dove Call-count Survey

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — The Mourning Dove (Zenaida macroura) Call-Count Survey was developed to provide an index to population size and to detect annual changes in mourning dove breeding...

  13. 2012 bobwhite whistle count : performance report

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — Performance report for the 2012 spring whistle count to monitor northern bobwhite abundance in Kansas state. This survey was initiated in 1998, and is preformed on...

  14. 2013 bobwhite whistle count : performance report

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — Performance report for the 2013 spring whistle count to monitor northern bobwhite abundance in Kansas state. This survey was initiated in 1998, and is preformed on...

  15. How to count an introduction to combinatorics

    CERN Document Server

    Allenby, RBJT

    2010-01-01

    What's It All About? What Is Combinatorics? Classic Problems What You Need to Know Are You Sitting Comfortably? Permutations and Combinations The Combinatorial Approach Permutations CombinationsApplications to Probability Problems The Multinomial Theorem Permutations and Cycles Occupancy Problems Counting the Solutions of Equations New Problems from Old A ""Reduction"" Theorem for the Stirling Numbers The Inclusion-Exclusion Principle Double Counting Derangements A Formula for the Stirling NumbersStirling and Catalan Numbers Stirling Numbers Permutations and Stirling Numbers Catalan Numbers Pa

  16. Statistical modelling for falls count data.

    Science.gov (United States)

    Ullah, Shahid; Finch, Caroline F; Day, Lesley

    2010-03-01

    Falls and their injury outcomes have count distributions that are highly skewed toward the right with clumping at zero, posing analytical challenges. Different modelling approaches have been used in the published literature to describe falls count distributions, often without consideration of the underlying statistical and modelling assumptions. This paper compares the use of modified Poisson and negative binomial (NB) models as alternatives to Poisson (P) regression, for the analysis of fall outcome counts. Four different count-based regression models (P, NB, zero-inflated Poisson (ZIP), zero-inflated negative binomial (ZINB)) were each individually fitted to four separate fall count datasets from Australia, New Zealand and United States. The finite mixtures of P and NB regression models were also compared to the standard NB model. Both analytical (F, Vuong and bootstrap tests) and graphical approaches were used to select