Sample records for blood biochemical baseline

  1. Baseline plasma corticosterone, haematological and biochemical results in nesting and rehabilitating loggerhead sea turtles (Caretta caretta). (United States)

    Flower, Jennifer E; Norton, Terry M; Andrews, Kimberly M; Nelson, Steven E; Parker, Clare E; Romero, L Michael; Mitchell, Mark A


    The evaluation of hormonal responses to stress in reptiles relies on acquisition of baseline corticosterone concentrations; however, the stress associated with the restraint needed to collect the blood samples can affect the results. The purpose of this study was to determine a time limit for the collection of blood samples to evaluate baseline corticosterone, haematological and biochemical results in nesting (n = 11) and rehabilitating (n = 16) loggerhead sea turtles (Caretta caretta). Blood samples were collected from the dorsal cervical sinus of each turtle immediately after touching the animal (t 0; 0-3 min) and 3 (t 3; 3-6 min), 6 (t 6; 6-9 min; nesting turtles only), 10 (t 10; 10-13 min) and 30 min (t 30; rehabilitating turtles only) after the initial hands-on time. Consistent between the rehabilitating and nesting turtles, there was a subtle yet significant increase in white blood cell counts over time. Despite the fact that white blood cell counts increased during the sampling period, there was no direct correlation between white blood cell count and corticosterone in the sampled turtles. In the nesting turtles, significant elevations in corticosterone were noted between t 0 and t 3 (P = 0.014) and between t 0 and t 6 (P = 0.022). Values at t 10 were not significantly different from those at t 0 (P = 0.102); however, there was a trend for the corticosterone values to continue to increase. These results suggest that sampling of nesting loggerhead sea turtles within 3 min of handling will provide baseline corticosterone concentrations in their natural environment. Significant elevations in corticosterone were also noted in the rehabilitating loggerhead sea turtles between t 0 and t 10 (P = 0.02) and between t 0 and t 30 of sampling (P = 0.0001). These results suggest that sampling of loggerhead sea turtles within 6 min of handling should provide baseline corticosterone concentrations in a rehabilitation setting. The

  2. Biochemical changes in blood under Cr6+


    Kuzenko E.V.; Romaniuk A.M.; Butko H.Yu.; Logvinova H.V.


    Background. For the manufacture of dentures many different alloys containing chromium are used. Interaction with oral fluid, organic acids and food, results in formation of Cr3+, Cr6+ ions, but their influence on the whole organism is poorly investigated. Objective. To analyze the biochemical changes in blood plasma during the influence of Cr6+ ions. Methods. 15 animals of experimental group were receiving drinking water with potassium dichromate in a dose of 0,2 mol/l. Rats of control group ...

  3. [Chronic fatigue syndrome: biochemical examination of blood]. (United States)

    Hakariya, Yukiko; Kuratsune, Hirohiko


    Though patients with chronic fatigue syndrome (CFS) have lots of complaints, abnormal findings cannot be detected by biochemical screening tests. However, some specialized blood tests have revealed neuroendocrine immune axis abnormalities, which is closely associated with each other. Recent studies indicate that CFS can be understood as a special condition based on abnormality of the psycho-neuro-endocrino-immunological system, with the distinguishing feature of CFS seeming to be the secondary brain dysfunction caused by several cytokines and/or autoantibodies. In this paper, we summarize these abnormalities found in CFS and show the neuro-molecular mechanism leading to chronic fatigue.

  4. Dynamics of biochemical parameters of blood serum in kidney injuries

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    Y. L. Podgainiy


    Full Text Available Aim. Annually injuries of varying severity are registered in more than 4,5 million people (up to 10% of the population in Ukraine; renal injury in polytrauma is detected in 26,4% of cases and takes 2 – 3 place of injury of the abdominal cavity and retroperitoneal space. In order to study the kidney function and other vital organs systems 108 patients were examined. Methods and results. Laboratory methods (clinical and biochemical parameters of blood and urine tests, ultrasound and CT scans of the kidneys and abdominal organs were used. Conclusion. It was established that polytrauma often occurs in males (73,5% of middle-age. 42% of patients presented renal function violation - nitrogen excretion and 84% of patients had activated blood coagulation in the first 7 – 10 days of injury.

  5. Biochemical analysis of CTLA-4 immunoreactive material from human blood

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    Dennert Kate


    Full Text Available Abstract Background CTLA-4 was initially described as a membrane-bound molecule that inhibited lymphocyte activation by interacting with B7.1 and B7.2 molecules on antigen presenting cells. Alternative splicing of mRNA encoding the CTLA-4 receptor leads to the production of a molecule (sCTLA-4 that lacks a membrane anchor and is therefore secreted into the extracellular space. Despite studies finding that people with autoimmune disease more frequently express high levels of sCTLA-4 in their blood than apparently healthy people, the significance of these findings is unclear. Methods Molecules isolated from blood using CTLA-4 specific antibodies were analyzed with ligand binding assays, mass spectroscopy, and biochemical fractionation in an effort to increase our understanding of CTLA-4 immunoreactive material. Results Mass spectroscopy analysis of the molecules recognized by multiple CTLA-4-specific antibodies failed to identify any CTLA-4 protein. Even though these molecules bind to the CTLA-4 receptors B7.1 and B7.2, they also exhibit properties common to immunoglobulins. Conclusion We have identified molecules in blood that are recognized by CTLA-4 specific antibodies but also exhibit properties of immunoglobulins. Our data indicates that what has been called sCTLA-4 is not a direct product of the CTLA-4 gene, and that the CTLA-4 protein is not part of this molecule. These results may explain why the relationship of sCTLA-4 to immune system activity has been difficult to elucidate.

  6. Baseline blood levels of manganese, lead, cadmium, copper, and zinc in residents of Beijing suburb

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    Zhang, Long-Lian, E-mail: [Department of Occupational Diseases Control and Prevention, Fengtai Center for Disease Control and Prevention, Beijing 100071 (China); Lu, Ling [Department of Occupational Diseases Control and Prevention, Fengtai Center for Disease Control and Prevention, Beijing 100071 (China); Pan, Ya-Juan; Ding, Chun-Guang [Institute for Occupational Health and Poison Control in China Center for Disease Prevention and Control, Beijing 100050 (China); Xu, Da-Yong [Department of Occupational Diseases Control and Prevention, Fengtai Center for Disease Control and Prevention, Beijing 100071 (China); Huang, Chuan-Feng; Pan, Xing-Fu [Institute for Occupational Health and Poison Control in China Center for Disease Prevention and Control, Beijing 100050 (China); Zheng, Wei, E-mail: [School of Health Sciences, Purdue University, West Lafayette, IN 47907 (United States)


    Baseline blood concentrations of metals are important references for monitoring metal exposure in environmental and occupational settings. The purpose of this study was to determine the blood levels of manganese (Mn), copper (Cu), zinc (Zn), lead (Pb), and cadmium (Cd) among the residents (aged 12–60 years old) living in the suburb southwest of Beijing in China and to compare the outcomes with reported values in various developed countries. Blood samples were collected from 648 subjects from March 2009 to February 2010. Metal concentrations in the whole blood were determined by ICP-MS. The geometric means of blood levels of Mn, Cu, Zn, Pb and Cd were 11.4, 802.4, 4665, 42.6, and 0.68 µg/L, respectively. Male subjects had higher blood Pb than the females, while the females had higher blood Mn and Cu than the males. There was no gender difference for blood Cd and Zn. Smokers had higher blood Cu, Zn, and Cd than nonsmokers. There were significant age-related differences in blood levels of all metals studied; subjects in the 17–30 age group had higher blood levels of Mn, Pb, Cu, and Zn, while those in the 46–60 age group had higher Cd than the other age groups. A remarkably lower blood level of Cu and Zn in this population as compared with residents of other developed countries was noticed. Based on the current study, the normal reference ranges for the blood Mn were estimated to be 5.80–25.2 μg/L; for blood Cu, 541–1475 μg/L; for blood Zn, 2349–9492 μg/L; for blood Pb, <100 μg/L; and for blood Cd, <5.30 μg/L in the general population living in Beijing suburbs. - Highlights: • Baseline blood levels of metals in residents of Beijing suburb are investigated. • BMn and BPb in this cohort are higher than those in other developed countries. • Remarkably lower blood levels of Cu and Zn in this Chinese cohort are noticed. • The reference values for blood levels of Mn, Cu, Zn, Pb, and Cd are established.

  7. Baseline albuminuria predicts the efficacy of blood pressure-lowering drugs in preventing cardiovascular events

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    Boersma, C.; Postma, M.J.; Visser, S.T.; Atthobari, J.; de Jong, P.E.; de Jong-van den Berg, L.T.; Gansevoort, R.T.


    AIMS Albuminuria has been proven to be associated with cardiovascular (CV) events in specific patient populations, but also in the general population. This study aimed to investigate whether the efficacy of blood pressure-lowering agents in preventing CV events depends on baseline urinary albumin ex

  8. Evaluation of the baseline Widal titres in healthy blood donors of Uttarakhand

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    Garima Mittal


    Full Text Available Introduction: The interpretation of Widal test depends upon the baseline titre which is prevalent amongst healthy individuals in a particular geographical area. Aim and Objective: The aim of this study was to determine the baseline Widal titre of the apparently healthy population of a tertiary care centre of Uttarakhand. Material and Methods: Blood samples were collected from healthy blood donors (n=500 of the age group of 18-60 years, of both the sexes, who attended our blood bank from September 2013 to November 2013 and were analyzed for the presence of Salmonella antibodies by carrying out the Widal tube agglutination test. Results: Of the 500 serum samples which were tested, 255 (51% serum samples were positive for agglutinins (≥ 1:20 and 245 were negative. The most frequently recorded titre of the reactive sera was 1:40 for the anti-O antibodies and 1:80 for the anti-H antibodies for Salmonella enterica serotype typhi and this was the baseline titre. While the baseline titre of the ‘H’ agglutinins of Salmonella enterica serotype paratyphi A was 1:20 and of paratyphi B was also1:20. Conclusions: Based on the results of our study, it has been recommended that the significant titre of 1:80 for the anti-O antibodies and of 1:160 for the anti-H antibodies may be considered as diagnostic for enteric fever in the region of Uttarakhand, India.

  9. Development of class model based on blood biochemical parameters as a diagnostic tool of PSE meat. (United States)

    Qu, Daofeng; Zhou, Xu; Yang, Feng; Tian, Shiyi; Zhang, Xiaojun; Ma, Lin; Han, Jianzhong


    A fast, sensitive and effective method based on the blood biochemical parameters for the detection of PSE meat was developed in this study. A total of 200 pigs were slaughtered in the same slaughterhouse. Meat quality was evaluated by measuring pH, electrical conductivity and color at 45min, 2h and 24h after slaughtering in M. longissimus thoracis et lumborum (LD). Blood biochemical parameters were determined in blood samples collected during carcass bleeding. Principal component analysis (PCA) biplot showed that high levels of exsanguination Creatine Kinase, Lactate Dehydrogenase, Aspertate aminotransferase, blood glucose and lactate were associated with the PSE meat, and the five biochemical parameters were found to be good indicators of PSE meat Discriminant function analysis (DFA) was able to clearly identify PSE meat using the five biochemical parameters as input data, and the class model is an effective diagnostic tool in pigs which can be used to detect the PSE meat and reduce economic loss for the company.

  10. Changes in blood biochemical markers before, during, and after a 2-day ultramarathon

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    Arakawa K


    damage parameters (uric acid, creatine kinase, lactate dehydrogenase, and aspartate aminotransferase, renal function markers (creatinine and blood urea nitrogen, liver injury index (alanine aminotransferase, lipid metabolism indices (free fatty acid, reactive oxygen species and inflammation parameters (white blood cells, interleukin-6, and C-reactive protein, and energy production and catecholamines (adrenaline, noradrenaline, and dopamine. Third pattern index of a lipid metabolism marker – triglyceride – decreased during the race periods and started returning to baseline from then onward. Some hormonal markers such as insulin, leptin, and adiponectin showed unique patterns. These findings appeared informative for nonprofessional athletes to know about an optimal physical activity level, duration, and total exercise for elevating physical performance and monitoring physical/mental conditioning as well as for prevention of overtraining and physical injuries. Keywords: long-distance running, blood biochemical markers, anemia, muscle damage, lipid metabolism, inflammation

  11. Diurnal variation in baseline human regional cerebral blood flow demonstrated by PET

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    Diehl, D.J.; Mintun, M.A.; Moore, R.Y. [Univ. of Pittsburgh, PA (United States)] [and others


    We have previously described the diurnal variation in regional cerebral blood flow (rCBF) response to bright light in human subjects as demonstrated by the positron emission tomography (PET) activation method. In this abstract, we report the differences in rCBF (an indicator of differences in regional neuronal activity) between the evening and midday dim light baseline scans which served as the control states in the above bright light activation study. Five right-handed, healthy volunteers underwent both an evening (8pm) and a midday (12N) O-15 water PET scanning session. Each scanning session was preceded by one hour of dim light adaptation (50 lux) and consisted of six rCBF scans at three different light intensities in an AABBCC sequence (A=50 lux, B=2500 lux, C=7000lux). Significant differences in rCBF between the evening and midday 50 lux states were identified using the statistical parametric mapping method developed by Friston et al (p<.001). The evening scans demonstrated areas of greater relative blood flow in the pineal gland, the lateral temporal cortex bilaterally, the right lateral prefrontal cortex, the superior aspect of the anterior cingulate, and the left thalamus. The midday scans showed areas of greater relative blood flow in the visual cortex, the left lateral prefrontal cortex. the inferior aspect of the anterior cingulate, the left parietal cortex and the cerebellum. Our results demonstrate an extensive diurnal variation in baseline human rCBF. This indicates that time of day may be an important variable in conducting and interpreting functional brain imaging studies. Furthermore, these results suggest possible neuroanatomical substrates through which the circadian system may regulate the various physiologic and behavioral processes that manifest circadian rhythms.


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    Lodyanov V. V.


    Full Text Available The research was conducted at the pure-bred pigs CT and hybrid juveniles CT x L Total protein blood serum was determined refractometrically, protein fraction - a method of horizontal electrophoresis on paper, the level of total lipids - B. Swahn, I. Scand. Researched transaminases (AST and ALT method of Reitman-Frenkel, alkaline phosphatase - method O.A. Bessey e.a., creatine kinase - S.S. Kuby. Cortisol levels was determined by radioimmunoassay analysis, adrenaline - fluorometric method. Phagocytic activity was installed by Ms. V. Matusevich, bactericidal activity - by the method of Overmoney, T.A. Kuzmina

  13. Red Blood Cell Susceptibility to Pneumolysin: CORRELATION WITH MEMBRANE BIOCHEMICAL AND PHYSICAL PROPERTIES. (United States)

    Bokori-Brown, Monika; Petrov, Peter G; Khafaji, Mawya A; Mughal, Muhammad K; Naylor, Claire E; Shore, Angela C; Gooding, Kim M; Casanova, Francesco; Mitchell, Tim J; Titball, Richard W; Winlove, C Peter


    This study investigated the effect of the biochemical and biophysical properties of the plasma membrane as well as membrane morphology on the susceptibility of human red blood cells to the cholesterol-dependent cytolysin pneumolysin, a key virulence factor of Streptococcus pneumoniae, using single cell studies. We show a correlation between the physical properties of the membrane (bending rigidity and surface and dipole electrostatic potentials) and the susceptibility of red blood cells to pneumolysin-induced hemolysis. We demonstrate that biochemical modifications of the membrane induced by oxidative stress, lipid scrambling, and artificial cell aging modulate the cell response to the toxin. We provide evidence that the diversity of response to pneumolysin in diabetic red blood cells correlates with levels of glycated hemoglobin and that the mechanical properties of the red blood cell plasma membrane are altered in diabetes. Finally, we show that diabetic red blood cells are more resistant to pneumolysin and the related toxin perfringolysin O relative to healthy red blood cells. Taken together, these studies indicate that the diversity of cell response to pneumolysin within a population of human red blood cells is influenced by the biophysical and biochemical status of the plasma membrane and the chemical and/or oxidative stress pre-history of the cell.

  14. Haematological and biochemical blood parameters in horses used for sport and recreation



    The study was conducted to determine and compare the resting values of haematological and biochemical blood indices in clinically healthy horses competing in show jumping and used for leisure activities. The experiment included twenty adult horses of Polish half-bred andWielkopolska breed which were subjected to a daily training programme typical of their type of use. Blood samples were collected at rest before feeding from the jugular external vein. Show jumpers had higher (P

  15. [The biochemical and rheological properties of the blood during the performance of angiography using Ultravist]. (United States)

    Poliaev, Iu A; Lazarev, V V; Kulikova, I S; Shimanovskiĭ, N L; Usenko, A N; Smirnova, O Iu; Isakov, Iu F; Sergeev, P V


    It is shown that Ultravist makes it possible to receive clear visualization of vessel channel in the zone "of interest" without changing functional conditions of heart vascular system and biochemical blood parameters (level of erythrocytes, bilirubine, urine nitrogen, activity of aspartataminotransferase). In concentration 30 mg/ml in vitro and in vivo Ultravist decreases a viscosity limit not affecting other rheological properties of blood. A mechanism of the found Ultravist effect and prospects of its application in practice for children are considered.

  16. Activity of the positive and negative reinforcement motivation systems and baseline arterial blood pressure in humans. (United States)

    Aftanas, L I; Sidorova, P V; Pavlov, S V; Makhnev, V P; Korenek, V V; Reva, N V; Amstislavskaya, T G


    The aim of the present work was to identify possible associations between individual balances in the activity of the positive and negative reinforcement motivation systems using a method based on emotional modulation of the startle reaction (EMSR) by motivationally significant emotionally positive and negative contextual visual stimuli and measures of cardiovascular system activity. Studies were performed using healthy males (mean age 30.29 +/- 9.8 years) with normal and first-episode excessive increases in arterial blood pressure (systolic blood pressure to greater than 140 mmHg, diastolic to greater than 90 mmHg). Cluster analysis of EMSR data identified groups of individuals with different activity profiles for the positive and negative reinforcement systems. Groups of subjects with changes in the balance of activity towards a lower level of positive reinforcement system activity (smaller startle reflexes to positive contextual stimuli) or a higher level of negative reinforcement system activity (larger startle reactions to threatening contextual stimuli) showed significantly greater baseline SBP and DBP. The possible mechanisms of the modulatory influences of the balance of system activities on autonomic vascular regulatory processes are discussed.

  17. Gcg-XTEN: an improved glucagon capable of preventing hypoglycemia without increasing baseline blood glucose.

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    Nathan C Geething

    Full Text Available OBJECTIVE: While the majority of current diabetes treatments focus on reducing blood glucose levels, hypoglycemia represents a significant risk associated with insulin treatment. Glucagon plays a major regulatory role in controlling hypoglycemia in vivo, but its short half-life and hyperglycemic effects prevent its therapeutic use for non-acute applications. The goal of this study was to identify a modified form of glucagon suitable for prophylactic treatment of hypoglycemia without increasing baseline blood glucose levels. METHODOLOGY/PRINCIPAL FINDINGS: Through application of the XTEN technology, we report the construction of a glucagon fusion protein with an extended exposure profile (Gcg-XTEN. The in vivo half-life of the construct was tuned to support nightly dosing through design and testing in cynomolgus monkeys. Efficacy of the construct was assessed in beagle dogs using an insulin challenge to induce hypoglycemia. Dose ranging of Gcg-XTEN in fasted beagle dogs demonstrated that the compound was biologically active with a pharmacodynamic profile consistent with the designed half-life. Prophylactic administration of 0.6 nmol/kg Gcg-XTEN to dogs conferred resistance to a hypoglycemic challenge at 6 hours post-dose without affecting baseline blood glucose levels. Consistent with the designed pharmacokinetic profile, hypoglycemia resistance was not observed at 12 hours post-dose. Importantly, the solubility and stability of the glucagon peptide were also significantly improved by fusion to XTEN. CONCLUSIONS/SIGNIFICANCE: The data show that Gcg-XTEN is effective in preventing hypoglycemia without the associated hyperglycemia expected for unmodified glucagon. While the plasma clearance of this Gcg-XTEN has been optimized for overnight dosing, specifically for the treatment of nocturnal hypoglycemia, constructs with significantly longer exposure profiles are feasible. Such constructs may have multiple applications such as allowing for more

  18. Influence of gastrointestinal parasitism on biochemical variables in blood of laying hens

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    Josué P Topázio


    Full Text Available Objective. This study describes the influence of endoparasites and diet on biochemical variables in sera of brown laying hens. Materials and methods. Biochemical parameters (seric levels of cholesterol, triglycerides, total protein, uric acid, albumin, and alanine aminotransferase activity were assessed in blood samples of poultry. Three populations of laying hens of different properties (n=20 each represented our experimental design, from which blood (serum and feces were collected for biochemical and parasitological analyzes, respectively. Results. From 60 feces samples evaluated 49 were positive for the presence of parasites and 11 were negative. Among the positive samples, 23 had infection with Eimeria spp and the other 26 samples had mixed parasitism with Eimeria spp and Heterakis gallinarum, which influenced (increasing the levels of total protein and globulin. However, when parasites were analyzed separately, it was observed that the infection by Eimeria spp affected parameters such as cholesterol (which increased, p=0.001 and triglycerides, showing lower results than when the hen was parasitized by Eimeria spp. (p=0.01. Significant (p<0.05 difference was observed in all biochemical variables when the three diets were compared. Conclusions. Our results allowed concluding that the parasitic infection and diet in laying hens influenced the results of serum biochemical parameters evaluated. The main conclusion we found that parasitism caused an increase in total protein and globulin (ie immune response, and there was a negative correlation between parasite load and albumin, uric acid and ALT.

  19. Whole blood interferon-gamma assay for baseline tuberculosis screening among Japanese healthcare students.

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    Katsuyuki Hotta

    Full Text Available BACKGROUND: The whole blood interferon-gamma assay (QuantiFERON-TB-2G; QFT has not been fully evaluated as a baseline tuberculosis screening test in Japanese healthcare students commencing clinical contact. The aim of this study was to compare the results from the QFT with those from the tuberculin skin test (TST in a population deemed to be at a low risk for infection with Mycobacterium tuberculosis. METHODOLOGY/PRINCIPAL FINDINGS: Healthcare students recruited at Okayama University received both the TST and the QFT to assess the level of agreement between these two tests. The interleukin-10 levels before and after exposure to M tuberculosis-specific antigens (early-secreted antigenic target 6-kDa protein [ESAT-6] and culture filtrate protein 10 [CFP-10] were also measured. Of the 536 healthcare students, most of whom had been vaccinated with bacillus-Calmette-Guérin (BCG, 207 (56% were enrolled in this study. The agreement between the QFT and the TST results was poor, with positive result rates of 1.4% vs. 27.5%, respectively. A multivariate analysis also revealed that the induration diameter of the TST was not affected by the interferon-gamma concentration after exposure to either of the antigens but was influenced by the number of BCG needle scars (p = 0.046. The whole blood interleukin-10 assay revealed that after antigen exposure, the median increases in interleukin-10 concentration was higher in the subgroup with the small increase in interferon-gamma concentration than in the subgroup with the large increase in interferon-gamma concentration (0.3 vs. 0 pg/mL; p = 0.004. CONCLUSIONS/SIGNIFICANCE: As a baseline screening test for low-risk Japanese healthcare students at their course entry, QFT yielded quite discordant results, compared with the TST, probably because of the low specificity of the TST results in the BCG-vaccinated population. We also found, for the first time, that the change in the interleukin-10 level after exposure to

  20. Neonatal nociception elevated baseline blood pressure and attenuated cardiovascular responsiveness to noxious stress in adult rats. (United States)

    Chu, Ya-Chun; Yang, Cheryl C H; Lin, Ho-Tien; Chen, Pin-Tarng; Chang, Kuang-Yi; Yang, Shun-Chin; Kuo, Terry B J


    Neonatal nociception has significant long-term effects on sensory perception in adult animals. Although neonatal adverse experience affect future responsiveness to stressors is documented, little is known about the involvement of early nociceptive experiences in the susceptibility to subsequent nociceptive stress exposure during adulthood. The aim of this study is to explore the developmental change in cardiovascular regulating activity in adult rats that had been subjected to neonatal nociceptive insults. To address this question, we treated neonatal rats with an intraplantar injection of saline (control) or carrageenan at postnatal day 1. The carrageenan-treated rats exhibited generalized hypoalgesia at basal state, and localized hyperalgesia after re-nociceptive challenge induced by intraplantar injections of complete Freund's adjuvant (CFA) as adults. Then we recorded baseline cardiovascular variables and 24-h responsiveness to an injection of CFA in the free-moving adult rats with telemetric technique. The carrageenan-treated rats showed significantly higher basal blood pressures (110.3±3.16 vs. control 97.0±4.28 mmHg). In control animals, baroreceptor reflex sensitivity (BRS) decreased, sympathetic vasomotor activity increased, and parasympathetic activity was inhibited after CFA injection. Blood pressure elevation was evident (107.0±2.75 vs. pre-injection 97.0±4.28 mmHg). Comparatively, the carrageenan-treated rats showed a higher BRS (BrrLF 1.03±0.09 vs. control 0.70±0.06 ms/mmHg) and higher parasympathetic activity [0.93±0.17 vs. control 0.32±0.02 ln(ms²)] after CFA injection. The change in blood pressure is negligible (111.9±4.05 vs. pre-injection 110.3±3.16 mmHg). Our research has shown that neonatal nociception alters future pain sensation, raises basal blood pressure level, and attenuates cardiovascular responsiveness to nociceptive stress in adult rats.

  1. The influences of SE infection on layers’ production performance, egg quality and blood biochemical indicators


    Fan, Shijie; Zheng, Jiangxia; Duan, Zhongyi; Yang, Ning; Xu, Guiyun


    Background Salmonella enterica serovar Enteritidis (SE), as a major cause of foodborn illness, infects humans mainly through the egg. However, the symptom of laying hens usually is not typical and hard to diagnosis. In the present study, it is studied that the influences of SE infection on layers’ performance, egg quality and blood biochemical indicators. It will help us to improve the strategy to control SE infection in commercial layers. One hundred layers at 20 wk of age were divided into ...

  2. The dynamics of blood biochemical parameters in cosmonauts during long-term space flights (United States)

    Markin, Andrei; Strogonova, Lubov; Balashov, Oleg; Polyakov, Valery; Tigner, Timoty

    Most of the previously obtained data on cosmonauts' metabolic state concerned certain stages of the postflight period. In this connection, all conclusions, as to metabolism peculiarities during the space flight, were to a large extent probabilistic. The purpose of this work was study of metabolism characteristics in cosmonauts directly during long-term space flights. In the capillary blood samples taken from a finger, by "Reflotron IV" biochemical analyzer, "Boehringer Mannheim" GmbH, Germany, adapted to weightlessness environments, the activity of GOT, GPT, CK, gamma-GT, total and pancreatic amylase, as well as concentration of hemoglobin, glucose, total bilirubin, uric acid, urea, creatinine, total, HDL- and LDL cholesterol, triglycerides had been determined. HDL/LDL-cholesterol ratio also was computed. The crewmembers of 6 main missions to the "Mir" orbital station, a total of 17 cosmonauts, were examined. Biochemical tests were carryed out 30-60 days before lounch, and in the flights different stages between the 25-th and the 423-rd days of flights. In cosmonauts during space flight had been found tendency to increase, in compare with basal level, GOT, GPT, total amylase activity, glucose and total cholesterol concentration, and tendency to decrease of CK activity, hemoglobin, HDL-cholesterol concentration, and HDL/LDL — cholesterol ratio. Some definite trends in variations of other determined biochemical parameters had not been found. The same trends of mentioned biochemical parameters alterations observed in majority of tested cosmonauts, allows to suppose existence of connection between noted metabolic alterations with influence of space flight conditions upon cosmonaut's body. Variations of other studied blood biochemical parameters depends on, probably, pure individual causes.

  3. Haematological and biochemical blood parameters in horses used for sport and recreation

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    Katarzyna BURLIKOWSKA


    Full Text Available The study was conducted to determine and compare the resting values of haematological and biochemical blood indices in clinically healthy horses competing in show jumping and used for leisure activities. The experiment included twenty adult horses of Polish half-bred andWielkopolska breed which were subjected to a daily training programme typical of their type of use. Blood samples were collected at rest before feeding from the jugular external vein. Show jumpers had higher (P<0.05 red blood cell count, haematocrit value and haemoglobin concentration as well as the serum level of total protein, albumin, α2- and γ-globulin, HDL and iron. The level of β- globulin, magnesium and activity of creatine kinase were significantly higher in recreationally used horses. There were no differences in the values of red blood cell parameters (MCV, MCH, MCHC, number of leucocytes and the percentage of their respective kinds, platelets, concentration of urea, creatinine, glucose, triglycerides, total cholesterol, LDL, activity of AST, ALT, LDH and in the content of Na, K, Cl, P, Ca between the tested groups. The results of the present study indicate that the type of training influences some of the resting haematological and biochemical indices in adult horses. Horses used for show jumping have higher aerobic capacity and show a more beneficial lipid profile compared to horses used for recreation.

  4. Baseline Systolic Blood Pressure Response to Exercise Stress Test Can Predict Exercise Indices following Cardiac Rehabilitation Program

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    Akram Sardari


    Full Text Available Background: Systolic blood pressure recovery (rSBP is of prognostic value for predicting the survival and co-morbidity rate in patients with coronary artery disease (CAD. This study investigated the association between rSBP and exercise indices after complete cardiac rehabilitation program (CR in a population-based sample of patients undergoing coronary artery bypass grafting (CABG.Methods: The sample population consisted of 352 patients who underwent pure CABG. The patients underwent standard symptom-limited exercise testing immediately before and also after the completion of the CR sessions. rSBP was defined as the ratio of the systolic blood pressure at 3 minutes in recovery to the systolic blood pressure at peak exercise.Results: An abnormal baseline rSBP after exercise was a strong predictor of exercise parameters in the last session, including metabolic equivalents (β = -0.617, SE = 0.127, p value < 0.001 and peak O2 consumption (β = -1.950, SE = 0.363, p value < 0.001 measured in the last session adjusted for baseline exercise characteristics, demographics, function class, and left ventricular ejection fraction.Conclusion: The current study strongly emphasizes the predictive role of baseline rSBP after exercise in evaluating exercise parameters following CR. This baseline index can predict abnormal METs value, peak O2 consumption, post-exercise heart rate, and heart rate recovery after a 24-session CR program.

  5. Contaminant concentrations, biochemical and hematological biomarkers in blood of West Indian manatees Trichechus manatus from Brazil. (United States)

    Anzolin, D G; Sarkis, J E S; Diaz, E; Soares, D G; Serrano, I L; Borges, J C G; Souto, A S; Taniguchi, S; Montone, R C; Bainy, A C D; Carvalho, P S M


    The West Indian manatee Trichechus manatus is threatened with extinction in Brazil, and this study focused on nondestructive blood samples analyzed for metals, polychlorinated biphenyls (PCBs) and organochlorine pesticides (OCPs), as well as biochemical and hematological biomarkers. Studied manatees were kept at Projeto Peixe-Boi headquarters in Pernambuco State, and at two natural areas in estuaries where they are released to the wild. Manatees kept at the natural estuary in Paraiba State have blood concentrations of Al, Pb, Cd, Sn that are 11, 7, 8 and 23 times greater, respectively, than the concentrations found in blood of animals from the same species in Florida, USA. An inhibition of butyrylcholinesterase in manatees kept at the two reintroduction sites in Alagoas and Paraiba States indicated possible exposure of the animals to cholinesterase inhibitor insecticides. PCBs and OCPs were not detected. Results from this study will help delineate conservation efforts in the region.

  6. Association of dietary pattern with biochemical blood profiles and bodyweight among adults with Type 2 diabetes mellitus in Tehran, Iran


    Darani Zad, Nasrin; Mohd Yusof, Rokiah; Esmaili, Haleh; Jamaluddin, Rosita; Mohseni, Fariba


    Background This study was conducted to identify dietary patterns and evaluated their association with biochemical blood profiles and body weight among adults with type 2 diabetes mellitus. Methods This was a cross sectional study conducted among 400 patients with type 2 diabetes mellitus in Tehran from March to August 2013. Biochemical blood profiles, socio-demographic, lifestyle, anthropometric measurements, and dietary data were obtained. Dietary data from food frequency questionnaire were ...

  7. Microwave Blood Thawing: Biochemical Analysis of Small Samples of Thawed Red Blood Cells. (United States)


    lactate + NAD+ ( Lehninger , 1977) The large increase in pyruvate observed at 6 hours post-wash was most likely due to the large lactate concentrations at...Storage of Blood. London: Academic Press. Lehninger , A.L. 1977. Biochemistry. New York: Worth Publishers, Inc. Lewis, G.P. 1965. Method using o-tolidine

  8. Effect of aspartame on biochemical and oxidative stress parameters in rat blood

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    Prokić Marko D.


    Full Text Available Aspartame (ASP is one of the most widely used nonnutritive sweeteners. This study investigates the chronic effects of ASP on hematological and biochemical parameters, and its effects on the oxidative/antioxidative status in the red blood cells of Wistar albino rats. Rats were provided with ASP (40 mg/kg/daily for six weeks in drinking water. Increased food and fluid intake was observed in the ASP-treated rats. Total body mass was significantly decreased in the ASP-treated rats. Treatment with ASP caused an increase in the concentrations of glucose, cholesterol, LDL-cholesterol, and in the activities of alanine aminotransferase (ALT, aspartate aminotransferase (AST and lactate dehydrogenase (LDH, as well as a decrease in the levels of HDL-cholesterol in the serum. A significant decline in the number of white blood cells (WBC was observed after ASP uptake. Based on the results we conclude that ASP induces oxidative stress, observed as an alteration of the glutathione redox status, which leads to increased concentrations of nitric oxide (NO and lipid peroxides (LPO in the red blood cells. Changes in biochemical parameters, lipid metabolism, as well as changes in the levels of oxidative stress markers and the appearance of signs of liver damage indicate that chronic use of ASP can lead to the development of hyperglycemia, hypercholesterolemia and associated diseases. [Projekat Ministarstva nauke Republike Srbije, br. 173041

  9. [Biochemical studies of blood serum use for diagnostics and efficacy of treatment evaluation of TMJ pain dysfunction]. (United States)

    Ibragimova, R S


    Study of biochemical indices of blood serum in patients with the pain disfunction of temporomandibular joint syndrome was performed. Analysis of biochemical studies of blood serum revealed the essential increase of adenozintriphosphate (ATPh) level on 17.5%, histamin--on 51.7%, stress hormones: adrenalin--on 76.9%, cortizol--on 51.4% in patients with the syndrome of pain disfunction of TMJ in comparison with the control group. Biochemical studies of blood revealed the more marked lowering of the level of histamin, adrenalin, cortizol in blood serum of patients after the treatment of alternative methods than after pharmaco- and physiotherapy. the high informative level of the studying of ATPh, histamin, kortisol of blood's serum for diagnostics and evaluation of the efficiency of treatment of the pain disfunction of temporomandibular joint syndrome was proved.

  10. Biochemical assessment of growth factors and circulation of blood components contained in the different fractions obtained by centrifugation of venous blood. (United States)

    Corigiano, M; Ciobanu, G; Baldoni, E; Pompa, G


    The aim of this study was to evaluate a biochemical marker with different elements of a normal blood serum and centrifuged blood serum after a different rotation system. For this technique, we used five fractions of a blood Concentrated Growth Factors system (bCGF) and a particular device for the different rotation program. Blood samples were collected from 10 volunteers aged between 35 and 55 in the Operative Unit of the “Sapienza” University of Rome with only a fraction of different biochemical elements. Through an individual blood phase separator tube of venous blood, active factions of serum and 4 fractions of red buffy coat were taken. The biochemical markers with 14 elements were examined at times: P1-11 minutes, P2-12minutes, P3-15 minutes. Exclusively biological materials which are normally applied in the regeneration techniques for different defects and lesions were used with this technique. After specific rotation programs, a different result was obtained for each cycle: P1, P2, P3. In test tubes obtained by separated blood, we observed a higher concentration of proteins, ions, and other antigens compared to normal blood plasma. Examining the biochemical results of different elements, we observed an increase (P≤0,01). Since each person’s DNA is different, we could not have the same results in 5 fractions of blood concentration, we did, however, find a good increase in only a fraction of proteins, immunoglobulin and different ions. We obtained five fractions after centrifugation, and we had an increase in different biochemical elements compared to normal blood (P≤0,01) which is significant at different times. These biochemical elements were stimulated by different growth factors, which are used by the immune system, and they induced the formation of hard and soft tissues and good regeneration.

  11. Use of Arius thalassinus fish in a pollution biomonitoring study, applying combined oxidative stress, hematology, biochemical and histopathological biomarkers: A baseline field study. (United States)

    Saleh, Yousef S; Marie, Mohamed-Assem S


    The present field study aimed to determine the extent of pollution in the Red Sea coast of Yemen Republic using a battery of biomarkers in sea catfish, Arius thalassinus, originating from a reference site in comparison with a polluted site. We reported the concentration of heavy metals in some vital fish organs and their effects on the morphological, hematological, biochemical and oxidative stress biomarkers accompanied by the examination of histopathological alterations. The obtained results showed clear signs of stress in fish from a polluted site. Linear correlation analysis exhibited that the biomarkers response could be linked to the detected metals bioaccumulation. In addition, principal component analysis showed a clear separation of sampling sites in two different assemblages. Semi-quantitative analysis for the observed histopathological lesions revealed that gills were the most affected organs with signs of severe alterations. This field investigation provides a baseline data on pollution status in this region.

  12. Effect of Sunflower cake inclusion on certain blood biochemicals of Japanese quails

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    Full Text Available A week old 120, Japanese quail chicks were equally and randomly distributed into four different treatment groups (T0, T1, T2 and T3, of three replicates having 10 chicks in each. All the chicks were reared on saw dust litter upto six weeks of age. The chicks in control group-T0 were fed corn-soya based diet with multi- enzyme and group T1, T2 and T3 were fed on diets containing 10, 20 and 30 per cent replaced soybean cake by sunflower cake with multi-enzyme supplementation, respectively. The significant (P< 0.01 differences were noticed between the treatments for weekly live body weights upto sixth week. The blood-biochemicals viz., serum glucose, serum total protein and serum cholesterol level did not varied significantly. It was concluded that inclusion of sunflower cake do not have any adverse effect on blood-biochemical parameters however supplementation of multi-enzyme improves numerically the growth performance of Japanese quails. [Vet. World 2010; 3(6.000: 289-290

  13. Biochemical Storage Lesions Occurring in Nonirradiated and Irradiated Red Blood Cells: A Brief Review

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    F. Adams


    Full Text Available Red blood cells undergo a series of biochemical fluctuations during 35–42-day storage period at 1°C to 6°C. The sodium/potassium pump is immobilised causing a decrease in intracellular potassium with an increase in cytoplasmic sodium levels, glucose levels decline, and acidosis occurs as a result of low pH levels. The frailty of stored erythrocytes triggers the formation of haemoglobin-containing microparticles and the release of cell-free haemoglobin which may add to transfusion difficulties. Lipid peroxidation, oxidative stress to band 3 structures, and other morphological and structural molecular changes also occur leading to spheroechinocytes and osmotic fragility. These changes that transpire in the red cells during the storage period are referred to as “storage lesions.” It is well documented that gamma irradiation exacerbates storage lesions and the reports of increased potassium levels leading to adverse reactions observed in neonates and infants have been of particular concern. There are, however, remarkably few systematic studies comparing the in vitro storage lesions of irradiated and nonirradiated red cell concentrates and it has been suggested that the impact of storage lesions on leucocyte reduced red blood cell concentrate (RBCC is incomplete. The review examines storage lesions in red blood cells and their adverse effects in reference to blood transfusion.

  14. Semiquantitative imaging measurement of baseline and vasomodulated normal prostatic blood flow using sildenafil (United States)

    Haaga, JR; Exner, A; Fei, B; Seftel, AD


    The physiologic variability of blood flow to the prostate has not been studied until this time. We report the vasoactive effects of sildenafil and phenylephrine on blood flow of the normal prostate. Sildenafil increases prostate blood flow by approximately 75% and phenylephrine reduces the flow incrementally. Administration of these drugs with dynamic contrast-enhanced magnetic resonance imaging may improve the diagnosis of cancerous tissue because according to the literature, tumor angiogenic vessels lack the vasoactive physiologic response of the normal tissue. PMID:16728965

  15. Investigation of some hematological and blood biochemical parameters in cattle spontaneously infected with bovine leukosis virus

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    Sandev Nikolay


    Full Text Available The aim of the present study was to follow out the alterations in some haematological and blood biochemical parameters in cattle spontaneously infected with enzootic bovine leukosis virus with regard to the invivodifferentiation of bovine leukosis stages. The experiment included 76 cows at various ages and body weight. Serological leukosis tests were done by agar-gel immunodiffusion test with a commercial kit of Synbiotiсs (France, containing standardised gp 51 antigen and positive serum approved by the EU. On the basis of haematological results, the cows were divided into three groups: first group – EBL-seropositive with normal haemogramme; second group – EBL seropositive with altered haemogramme and third group – controls. In cows from the first and the second group, a statistically significantly increased blood cell counts was established compared to healthy controls. The total WBC were increased in the second group (leukocytosis up to 33.21×109/l vs reference range of 5-10×109/l as well as lymphocyte percentages (lymphocytosis – 81.89% (reference 40–63%. A reduction in the proportion of neutrophils to 12.78% (relative neutropenia vs the reference range of 22-49% and monocytes (monocytopenia to 1.78% (reference range 2–6% was observed. A statistically significant reduction in Ca concentrations (4.41 mg/dl and higher inorganic phosphate levels (5.28 mg/dl were established in cows from the second group. Also, ASAT activity was considerably lower – 47.03 U/l, while alkaline phosphatase increased slightly within the reference range up to 167.68 U/l and 165.81 U/l in groups one and two, respectively. The present haematological and whole blood/serum biochemical results in cows spontaneously infected with EBL virus could be used as prognostic markers of the course of the disease, to distinguish the stages of infection with regard to alive diagnostics.

  16. Effect of age and gender on some blood biochemical parameters of apparently healthy small ruminants from Southern Punjab in Pakistan

    Institute of Scientific and Technical Information of China (English)

    Shumaila Kiran; Arif Mahmood Bhutta; Bakhtyawar Ali Khan; Sobia Durrani; Muhammad Ali; Muhammad Ali; Furhan Iqbal


    Objective: To report and compare the hematological and serum biochemical profile of goat and sheep from Rahim Yar Khan district in Southern Punjab. Methods: One hundred and twenty blood samples (98 goat and 22 sheep) were collected and their blood glucose, hemoglobin and serum biochemical parameters, cholesterol, aspartate transaminase (AST), alanine aminotransferase (ALT) and lactate dehydrogenase (LDH) were determined by using spectrophotometer. Parameters were compared between goat and sheep. Results: It was observed that glucose (P=0.001), LDH (P=0.001) and ALT (P=0.001) concentrations differed significantly between sheep and goats. The studied parameters were compared between buck and goats and also between young goats (less than 12 months old) and adults (more than 12 months old). It was found that age and gender did not affect these hematological and serum biochemical parameters.Conclusions: It can be concluded that sheep have overall higher values than goats for studied hematological and serum biochemical parameters.

  17. The influences of SE infection on layers’ production performance, egg quality and blood biochemical indicators

    Institute of Scientific and Technical Information of China (English)

    Shijie Fan; Jiangxia Zheng; Zhongyi Duan; Ning Yang; Guiyun Xu


    Background:Salmonella enterica serovar Enteritidis (SE), as a major cause of foodborn illness, infects humans mainly through the egg. However, the symptom of laying hens usually is not typical and hard to diagnosis. In the present study, it is studied that the influences of SE infection on layers’ performance, egg quality and blood biochemical indicators. It will help us to improve the strategy to control SE infection in commercial layers. One hundred layers at 20 wk of age were divided into 2 groups, 60 hens for experiment and others for control. The experiment group was fed with the dosage of 108 CFU SE per hen. The specific PCR was used to detect the deposition of SE. On the 8 d after SE infection, 10 hens from the control group and 30 hens from the experimental group were slaughtered to detect the SE colonization. The production performance, egg quality and blood biochemical indices were also analyzed. Results:The results showed that the colonization rate of SE was highest in caecum contents (55.17%) and lowest in vagina (17.24%). For the eggs the detection rate of SE was highest on the eggshell (80.00%) and lowest in yolk (18.81%). SE infection had no significant influence on production performance and egg qualities (P>0.05). The difference of laying rate between the experimental and control groups was less than 0.30%, and both were approximately equal to 82.00%. The blood analysis showed that the aspartic aminotransferase (AST) and alanine aminotransferase (ALT) of experimental group was significantly higher than those of control group (P<0.05). For experimental and control groups AST values were 236.22 U/l and 211.84 U/l respectively, and ALT values were 32.19 U/l and 24.55 U/l. All of coefficients were less than 20%. The colonization of SE in organs increases the enzyme activities of AST and ALT in blood. Conclusions:SE in feed could invade the oviduct and infect the forming eggs. It significantly increased the concentration of ALT and AST in blood

  18. Baseline blood Pb levels of black-necked stilts on the upper Texas coast (United States)

    Riecke, Thomas V.; Conway, Warren C.; Haukos, David A.; Moon, Jena A.; Comer, Christopher E.


    There are no known biological requirements for lead (Pb), and elevated Pb levels in birds can cause a variety of sub-lethal effects and mortality. Historic and current levels of Pb in mottled ducks (Anas fulvigula) suggest that environmental sources of Pb remain available on the upper Texas coast. Because of potential risks of Pb exposure among coexisting marsh birds, black-necked stilt (Himantopus mexicanus) blood Pb concentrations were measured during the breeding season. Almost 80 % (n = 120) of 152 sampled stilts exceeded the background threshold (>20 μg/dL) for Pb exposure. However, blood Pb concentrations did not vary by age or gender, and toxic or potentially lethal concentrations were rare (blood Pb concentrations of black-necked stilts in this study suggest the presence of readily bioavailable sources of Pb, although potential impacts on local stilt populations remain unclear.


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    Full Text Available Objective: The biochemical characterization of the substances presented in the new haemo and immuno stimulating preparation Tortezin from the blood cells of the Central Asian tortoise. Materials and Methods: Tortezin substances manufactured in 20062010 were examined by electrophoresis, spectroscopy and RPHPLC. Results: In UV spectra of Tortezin aqueous solution the pronounced maximum was at 218±1.2 nm and mild spike at 280±3.2 nm; in fluorescence emission spectra upon excitation with wavelength at 280 nm peak emission maximum was 460 ± 38 nm and at 295 nm was 455±21 nm. The RPHPLC profile and SDS 10% PAGE showed the presence of repetitive typical peaks in different samples. Conclusion: The different methods are to be recommended for standardization of Tortezin


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    Katarína Zbyňovská


    Full Text Available The most important and the most common Fusarium mycotoxin is deoxynivalenol (DON. It occurs predominantly in grains such as wheat, barley, and maize and less often in oats, rice, rye, sorghum and triticale. It has adverse effects on humans, animals, and crops that result in illnesses and economic losses. The aim of the present study was to investigate the effect of DON on some haematological (red blood cells - RBC, white blood cells - WBC, platelets - PLT, haemoglobin - HGB, packed cell volume - PCV and lymphocyte - LYM, biochemical (cholesterol, triglycerides, total protein, urea, calcium and phosphorus and anti- and pro-oxidants parameters (superoxide dismutase - SOD, glutathione peroxidase - GPx and ROS – reactive oxygen species in porcine blood in vitro. Significantly decreased content of total protein in the group with dose of 1000 ng.l-1 DON was observed compared with the control group. In other groups (E1 with 10 ng.l-1 DON and E2 with 100 ng.l-1 slightly lower values were measures in comparison with the control group. PLT significantly decreased in the experimental group E3 when compared with E1, E2, and the control group. Concentration of GPx in porcine blood significantly (P < 0.05 decreased in E1 against the control group. Concentration of SOD significantly (P < 0.05 decreased in group E2 in comparison with E1 group. The highest value of ROS was in E2 group. Other parameters were not influenced by The most important and the most common Fusarium mycotoxin is deoxynivalenol (DON. It occurs predominantly in grains such as wheat, barley, and maize and less often in oats, rice, rye, sorghum and triticale. It has adverse effects on humans, animals, and crops that result in illnesses and economic losses. The aim of the present study was to investigate the effect of DON on some haematological (red blood cells - RBC, white blood cells - WBC, platelets - PLT, haemoglobin - HGB, packed cell volume - PCV and lymphocyte - LYM, biochemical

  1. Blood biochemical parameters in male American mink (Neovison vison before and during the breeding season

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    Bogdan Lasota


    Full Text Available This study was aimed at selected blood biochemical parameters in the male American mink before (September-November and during the breeding (January-March season. Blood from 143 Black and Sapphire male mink at one year age was collected. The plasma was assayed for the concentration of glucose, total protein, cholesterol, HDL and LDL fractions, triacylglycerides (TG, and the activity of ALT and AST. Concentrations of glucose, protein, total and HDL/LDL cholesterol, and AST activity were generally slightly higher during the breeding season than during the non-breeding season, but remained within the reference range. In the case of ALT activity and TG concentration, the relations were reversed. The parameters studied in the Sapphire mink showed greater variation, both in- and out of the season. In Black and Sapphire males of the American mink, the studied parameters revealed slightly higher values during the breeding season than the non-breeding season. This will vary depending on the color variety. The decrease in TG concentration during the breeding season may indicate an increased energy demand due to ambient temperature falls, and/or may be a sign of increased energy consumption associated with physical exertion during mating. Consideration should be given to the nutrition of male mink during the breeding season.

  2. [Changes of physiological and biochemical characteristics of rat erythrocytes after blood loss]. (United States)

    Maslova, M N; Kazennov, A M; Katiukhin, L N; Novozhilov, A V; Skverchinskaia, E A; Tavrovskaia, T V


    In experiments of Wistar male rats, changes are studied of erythrocyte hematological, biochemical (activitities of transport ATPases), and rheological properties (capability for aggregation and deformability) 7 days after bloodletting of 12-15 % of the total blood mass. It has been shown that alongside with an elevation of erythrocyte volume and of the number of immature cells - reticulocytes, there was a statistically significant increase of Na,K-ATPase and Ca-ATPase activities in the whole erythrocytes and their membrane preparations - ghosts, the increment of activity in the case of Na,K-ATPase being essentially higher in the whole cells. This indicates the appearance of an enzyme activator inside the erythrocytes. There are also revealed a decrease of firmness of erythrocyte aggregates, a deceleration of spontaneous aggregation, and an increase of index of erythrocyte deformability. The conclusion is made that changes of erythrocyte rheological properties are interconnected with changes of the Na,K-ATPase activity and are directed to optimization of blood circulation in large vessels and capillary network.

  3. The effect of intramuscular injection of spiramycin at therapeutic dose on some blood biochemical and hematological parameters in Assaf sheep

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    Ibrahim M. ALZuheir


    Full Text Available Spiramycin is used to treatment of different bacterial and protozoal infection in different animal species including sheep, to the authors’ knowledge there are no studies about effects of spiramycin in sheep blood biochemical and hematological parameters. This study was designated to determine the effects of spiramycin intramuscular treatment at therapeutic dose (64,000 IU/kg for five days in some blood biochemical and hematological parameters in healthy Assaf sheep (n=8. The results showed that spiramycin treatment caused decrease in calcium and creatinine level (PP>0.05. After treatment, hematological parameters tend to decrease toward normal references range of red blood cell count, haemoglobin concentration, mean corpuscular hemoglobin, mean corpuscular hemoglobin concentration and red blood cell distribution width (PP>0.05. All the measured biochemical and hematological parameters were in the normal references range after the treatment. These results suggested that spiramycin given in therapeutic regimen to healthy Assaf sheep caused only minor inconclusive changes in the measured hematological and biochemical profiles; and thus can be used safely in treating susceptible infections in sheep. These results might be accepted as a starting point for future experiments to evaluate the effects spiramycin on the different systems and parameters.  

  4. Effects of blood pressure lowering on cardiovascular risk according to baseline body-mass index : a meta-analysis of randomised trials

    NARCIS (Netherlands)

    Ying, A.; Arima, H.; Czernichow, S.; Woodward, M.; Huxley, R.; Turnbull, F.; Perkovic, V.; Neal, B.; Agodoa, L.; Estacio, R.; Schrier, R.; Lubsen, J.; Chalmers, J.; Cutler, J.; Davis, B.; Wing, L.; Poulter, N. R.; Sever, P.; Remuzzi, G.; Ruggenenti, P.; Nissen, S.; Lindholm, L. H.; Fukui, T.; Ogihara, T.; Saruta, T.; Black, H.; Sleight, P.; Lievre, M.; Suzuki, H.; Fox, K.; Lisheng, L.; Ohkubo, T.; Imai, Y.; Yusuf, S.; Bulpitt, C. J.; Lewis, E.; Brown, M.; Palmer, C.; Wang, J.; Pepine, C.; Ishii, M.; Yui, Y.; Kuramoto, K.; Pfeff, M.; Asselbergs, F. W.; van Gilst, W. H.; Byington, B.; Pitt, B.; Brenner, B.; Remme, W. J.; de Zeeuw, D.; Rahman, M.; Viberti, G.; Teo, K.; Zanchetti, A.; Malacco, E.; Mancia, G.; Staessen, J.; Fagard, R.; Holman, R.


    Background The cardiovascular benefits of blood pressure lowering in obese people compared with people of normal weight might depend on choice of drug. We compared the effects of blood pressure-lowering regimens on cardiovascular risk in groups of patients categorised by baseline body-mass index (BM

  5. Developmental dynamics of some blood biochemical parameters in the growing turkey (Meleagris gallopavo). (United States)

    Szabó, A; Mézes, M; Horn, P; Süto, Z; Bázár, Gy; Romvári, R


    Blood serum clinical biochemical parameters of fasted BUT Big 8 male turkeys were determined at the ages of 3 days, 4, 8, 12, 16 and 20 weeks, for a follow-up of the developmental changes of some serum metabolites, enzymes and ions. The serum protein content (total protein, albumin, globulin) increased with age, indicating also the moulting-associated metabolic changes in the age interval from the 8th to the 12th weeks. Creatinine was shown to have a peak at 3 days of age (role of muscle activity in thermogenesis), while urate concentration sensitively reflected the dietary protein amount. Serum triglycerides peaked at the time of yolk catabolism, while cholesterol was shown to indicate the moulting, as was serum malondialdehyde. Serum sodium content increased throughout the study. Alanine aminotransferase and aspartate aminotransferase activities increased along the ontogeny, while alkaline phosphatase activity decreased in parallel with the growth. Serum creatine kinase activity showed an over one-magnitude increase. General metabolic and enzymatic alterations were characteristic and applicable for the description of the ontogenetic development of a precocial (post-hatch triglyceride peak), large bodied, meat-type (lactate dehydrogenase, continuously increasing creatine kinase) bird species.

  6. The Effects of Ethanol on the Morphological and Biochemical Properties of Individual Human Red Blood Cells.

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    Sang Yun Lee

    Full Text Available Here, we report the results of a study on the effects of ethanol exposure on human red blood cells (RBCs using quantitative phase imaging techniques at the level of individual cells. Three-dimensional refractive index tomograms and dynamic membrane fluctuations of RBCs were measured using common-path diffraction optical tomography, from which morphological (volume, surface area, and sphericity; biochemical (hemoglobin (Hb concentration and Hb content; and biomechanical (membrane fluctuation parameters were retrieved at various concentrations of ethanol. RBCs exposed to the ethanol concentration of 0.1 and 0.3% v/v exhibited cell sphericities higher than those of normal cells. However, mean surface area and sphericity of RBCs in a lethal alcoholic condition (0.5% v/v are not statistically different with those of healthy RBCs. Meanwhile, significant decreases of Hb content and concentration in RBC cytoplasm at the lethal condition were observed. Furthermore, dynamic fluctuation of RBC membranes increased significantly upon ethanol treatments, indicating ethanol-induced membrane fluidization.

  7. The Effects of Ethanol on the Morphological and Biochemical Properties of Individual Human Red Blood Cells (United States)

    Lee, Sang Yun; Park, Hyun Joo; Best-Popescu, Catherine; Jang, Seongsoo; Park, Yong Keun


    Here, we report the results of a study on the effects of ethanol exposure on human red blood cells (RBCs) using quantitative phase imaging techniques at the level of individual cells. Three-dimensional refractive index tomograms and dynamic membrane fluctuations of RBCs were measured using common-path diffraction optical tomography, from which morphological (volume, surface area, and sphericity); biochemical (hemoglobin (Hb) concentration and Hb content); and biomechanical (membrane fluctuation) parameters were retrieved at various concentrations of ethanol. RBCs exposed to the ethanol concentration of 0.1 and 0.3% v/v exhibited cell sphericities higher than those of normal cells. However, mean surface area and sphericity of RBCs in a lethal alcoholic condition (0.5% v/v) are not statistically different with those of healthy RBCs. Meanwhile, significant decreases of Hb content and concentration in RBC cytoplasm at the lethal condition were observed. Furthermore, dynamic fluctuation of RBC membranes increased significantly upon ethanol treatments, indicating ethanol-induced membrane fluidization. PMID:26690915

  8. 血液生化检验的临床调查研究%Blood Biochemical Examination of Clinical Research

    Institute of Scientific and Technical Information of China (English)



    Objective To explore the influence factors of clinical blood biochemical tests and their results accuracy.MethodsColect the hospital clinical laboratory in the near future keep biochemical blood test data of 80 patients. Analysis of the emergence of al patients in the whole process of the blood colected, for the blood biochemical test, the influence of test analysis explore the influencing factors on the accuracy of the blood test.Results When sampling before patients taking drugs, improper sampling method, for the amount of colection after anticoagulation is not correct, preservation conditions and inspection time is not scientific, the stand or fal of instrument work are important factors influencing the accuracy of the blood biochemical tests.Conclusion For patients with blood biochemical examination must be in strict accordance with the blood test, the relevant provisions of the can obtain accurate blood test results.%目的:探究临床血液生化检验的影响因素及其结果准确性。方法收集我院检验科近期保存的80份患者生化验血资料。分析所有患者采集血液的全程出现的情况对血液生化检验的影响,结合检验分析探讨对血液检验的准确性的影响因素。结果采集前患者服用药物、采集时采集方法不当,采集后抗凝使用不正确、保存条件和送检时间不科学、仪器工作的好坏都是影响血液生化检验准确性的重要因素。结论对患者进行血液生化检验时必须严格按照血液检验的相关规定,才能获得准确的血液检验结果。

  9. Antihypertensive medications and sexual function in women: Baseline data from the Systolic Blood Pressure Intervention Trial (SPRINT) (United States)

    Thomas, Holly N.; Evans, Gregory W.; Berlowtiz, Dan R.; Chertow, Glenn M.; Conroy, Molly B.; Foy, Capri G.; Glasser, Stephen P.; Lewis, Cora E.; Riley, William T.; Russell, Laurie; Williams, Olubunmi; Hess, Rachel


    Objectives Hypertension is a risk factor for the development of cardiovascular and kidney disease, but treatment can substantially reduce risks. Many patients avoid antihypertensive medications due to fear of side effects. While associations between antihypertensives and sexual dysfunction in men have been documented, it remains unclear whether antihypertensives are associated with sexual dysfunction in women. We conducted a cross-sectional analysis of baseline data from women in the Systolic Blood Pressure Intervention Trial (SPRINT) to evaluate the relations among class of antihypertensive medication and the outcomes (a) sexual activity and (b) sexual function. Methods SPRINT enrolled individuals 50 and older with hypertension at high risk for cardiovascular disease. A subset of participants completed questionnaires regarding quality of life (QoL), including sexual function. Antihypertensive class was determined by medications taken at baseline. Results Of 690 women in the QoL subset of SPRINT, 183 (26.5%) were sexually active. There were no significant differences in sexual activity among women taking one or more antihypertensives and women not taking any. Women taking an angiotensin-converting enzyme inhibitor or angiotensin receptor blocker (ACEI/ARB) had higher odds of sexual activity [OR 1.66 (1.12-4.27), p=0.011]. Among sexually active women, the prevalence of sexual dysfunction was high (52.5%). No class of medication was associated with sexual dysfunction in the multivariable model. Conclusions ACEI/ARB use was associated with higher odds of sexual activity. While prevalence of sexual dysfunction was high, no single class of antihypertensive medication was associated with sexual dysfunction. PMID:27032074

  10. The Effects of Air Pollution and Smoking on Cadmium Concentration in Human Blood and Correlation with Biochemical Parameters

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    L. Zeneli


    Full Text Available Problem statement: The study described the research of the effects that the environment pollution and smoking have in cadmium concentration in human blood, as well as in the correlation between cadmium and the biochemical parameters. Approach: In a comparative study of cadmium concentration in blood of human population of two different environments in Kosovo, one nearby Kosovo Thermo Power Plants (Obiliq, a highly polluted environments (Investigated Group and the other that was considered as relatively clean rural environment Dragash (control group. Results: The results showed that there exists a significant difference in the average concentration of cadmium in human blood between the Investigated Group (IG and the Control Group (CG (t = -3.34, p = 0.0006. The series of determination of cadmium concentration in blood of population that lives in this environment had shown direct effects in biochemical parameters (direct bilirubine, total bilirubine. Conclusion: Air pollution (from coal burning in power plant and smoking were very important factors for the level of cadmium concentration in blood, which had an inhibitory effect in the syntheses of bilirubine.

  11. Effect of feeding different levels of Azolla pinnata on blood biochemicals, hematology and immunocompetence traits of Chabro chicken

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    Deepesh Bharat Mishra


    Full Text Available Aim: The present study was conducted to see the effect of feeding different levels of Azolla meal on blood biochemicals, hematology and immunocompetence traits of Chabro chicken. Materials and Methods: The study was conducted on 160 Chabro chicks, which were randomly divided into four treatment groups each with four replicates of 10 birds. The first treatment (T1 served as a control in which basal diets was offered without Azolla supplementation while in T2, T3, and T4 groups, basal diet was replaced with Azolla meal at 5%, 7.5%, and 10% levels, respectively. A feeding trial was conducted upto 8 weeks. At the last week of trial, blood samples were collected randomly from one bird of each replicate and plasma was separated to estimate certain biochemical parameters, some blood metabolites, minerals and enzymes like alanine aminotransferase and aspartate aminotransferase (AST. Hematological parameters such as hemoglobin, packed cell volume, total leukocytes count and differential leukocytes count were estimated in fresh blood just after collection. The humoral immune response was measured against sheep red blood cells,and cell-mediated immune response was measured against phyto hemagglutinin lectin from Phaseolus vulgaris (PHA-P. Results: The study showed that hematological profile of the Chabro bird was not affected by any treatment except heterophil and lymphocyte which was found higher in T2 and T3 groups and eosinophil was found higher in a T3 group than control. Blood glucose, creatinine, cholesterol, total protein, albumin, uric acid, and triglycerides were found similar in all the groups and within the normal values for broiler chicken. Liver enzymes and macro mineral content in blood were found similar in all the treatment groups and within normal physiological range. Although AST was found higher in 10% replacement group than control, the value was within normal range for broiler chicken. Although antibody titer was found similar in all


    Directory of Open Access Journals (Sweden)

    Nikolay Kornilov


    Full Text Available In the article the results of comparative analysis of the composition of the Eurasian hydromineral resources and the assessment of their impact on the physiological condition of a human organism according to biochemical studies of venous blood are presented. Processing of initial data on the composition and properties of mineral waters chloride-hydrocarbonate, sulphate- hydrocarbonate and chloride-sulphate types and venous blood are made using the method of mathematical modeling, developed by the authors of this article. It is shown that in the balneological impact of hydromineral resources on the body in the blood increases the hemoglobin and oxygen, decreases glucose, and acid-base pH shifted to high alkalinity.

  13. Characterization of blood biochemical markers during aging in the Grey Mouse Lemur (Microcebus murinus: impact of gender and season

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    Marchal Julia


    Full Text Available Abstract Background Hematologic and biochemical data are needed to characterize the health status of animal populations over time to determine the habitat quality and captivity conditions. Blood components and the chemical entities that they transport change predominantly with sex and age. The aim of this study was to utilize blood chemistry monitoring to establish the reference levels in a small prosimian primate, the Grey Mouse Lemur (Microcebus murinus. Method In the captive colony, mouse lemurs may live 10–12 years, and three age groups for both males and females were studied: young (1–3 years, middle-aged (4–5 years and old (6–10 years. Blood biochemical markers were measured using the VetScan Comprehensive Diagnostic Profile. Because many life history traits of this primate are highly dependent on the photoperiod (body mass and reproduction, the effect of season was also assessed. Results The main effect of age was observed in blood markers of renal functions such as creatinine, which was higher among females. Additionally, blood urea nitrogen significantly increased with age and is potentially linked to chronic renal insufficiency, which has been described in captive mouse lemurs. The results demonstrated significant effects related to season, especially in blood protein levels and glucose rates; these effects were observed regardless of gender or age and were likely due to seasonal variations in food intake, which is very marked in this species. Conclusion These results were highly similar with those obtained in other primate species and can serve as references for future research of the Grey Mouse Lemur.

  14. Changes of blood biochemical indexes in stroke patients after scalp acupuncture:A randomized controlled observation

    Institute of Scientific and Technical Information of China (English)

    Hongxing Zhang; Li Zhou; Tangfa Zhang; Guofu Huang


    perform acupuncture successively with 3 needles.At approximately 30.angle,the needle was quickly inserted beneath the scalp.When the needlepoint reached the lower layer of galea aponeurotica and less resistance was felt by fingers,the needle was inserted horizontally as scalp for another 1.0-1.5 cun,then twirled rapidly and continuously at the speed of about 220 times per minute. Afterwards the needle was retained for thirty minutes and twirled once every five minutes, and every twirling lasted for two or three minutes.During the retention,the affected part should be moved about either actively or passively.②Body acupuncture group:The acupoints were Jianyu(LI 15),Quchi(LI 11),Hegu(LI 14),Waiguan(SJ 5),Huantiao (GB 30),Yanglingquan(GB 34),Zusanli(ST 36)and Sanyinjiao(SP 6),etc.,and eight to ten acupoints of them were inserted each time using acupuncture needles(1.0-4.0 cun).Having brought about the needling sensation in acupuncture treatment,uniform reinforcing-reducing method was conducted.The needles were also retained for 30 minutes with hand manipulating of the needles once every five minutes. Both groups received treatments once a day.and six treatments as a course,there was a one-day interval between two courses,continuously for 4 courses. ③The Criteria for Diagnosis and Therapeutic Effect Evaluation of Apoplexy was applied to evaluate the curative effects of all the patients after 4 courses.④At one day before the treatment and after four courses of treatment,the hemorrheologic indexes were assayed with LBY-N6 hemorrheologic apparatus produced by Tianjin Techcomp Ltd:And blood lipids were tested with automatic biochemical analyzer,while radioimmunoassay(RIA)was used to measure the level of plasma endothelin.⑤Difference of ranked data were compared by means of Radit analysis.And t test was used for comparison between two groups while pairing t test was used for comparison of enumeration data before and after treatment.MAIN OUTCOME MEASURES: ①Comparison of

  15. Effects of dietary administration of yarrow extract on growth performance and blood biochemical parameters of rainbow trout (Oncorhynchus mykiss

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    Mahmoud Nafisi Bahabadi


    Full Text Available The present study was conducted to investigate the clinical effects and possible side effects of yarrow extract (Achillea millefolium L. as feed additive on biochemical blood parameters and growth performance of rainbow trout (Oncorhynchus mykiss. Fishes were treated with 0 (control, 0.1, 0.5 and 1% of yarrow extract for 30 days. Plasma alanine aminotransferase (ALT, aspartate aminotransferase (AST, lactate dehydrogenase (LDH, alkaline phosphatase (ALP, creatine kinase (CK, peroxidase activity, total complement and lysozyme activity, glucose, total protein, triglyceride and cholesterol were measured after 15 and 30 days of yarrow treatment. There were no significant changes in the lysozyme activity and glucose levels. Total protein and globulin levels were significantly higher in the fish fed with diets enriched with 1% yarrow extract on day 30. Triglyceride and cholesterol levels was significantly decreased in the fish fed with diets containing 0.5% and 1% yarrow extract on day 30 (P<0.05. LDH, CK and peroxidase activities in the fish fed with diets having 1% yarrow extract were significantly decreased at the end of the experiment (P<0.05. In contrast, a significant increase in AST, ALP and total complement activity was observed in the fish fed with 1% yarrow extract diet, on day 15 (P<0.05. The weight gain and specific growth rate increased and food conversion ratio decreased in in the fish fed 1% yarrow extract on day 30. Condition factor in the fish fed with yarrow extract was significantly higher than control group on 30 day. In conclusion, on the basis of these results, oral administration of yarrow extract up to 0.5% have not side effect on blood biochemical and clinical parameters of fishes. However, oral administration of 1% of yarrow extract caused cytotoxicity and modifications in blood biochemical parameters of fish.

  16. Meat Characteristics, Fatty Liver Weight and Blood Biochemical Parameters in Force-Feeding Geese



    A study on growth performance, meat characteristics, fatty liver weight and serum biochemical parameters (ASAT, ALAT, alkaline phosphatase, γ-glutamyltransferase, total cholesterol, triglycerides and creatinine) in Landes geese before and after force-feeding was carried out. The overfeeding of geese with corn was taken after 90-days of age. Frequency of daily force feeding was gradually increased from 2 to 5 until the 15th day of force feeding. During force-feeding period, the body weight inc...

  17. Effect of humic-plant feed preparations on biochemical blood parameters of laying hens in deep litter housing system. (United States)

    Bubel, F; Dobrzański, Z; Gaweł, A; Pogoda-Sewerniak, K; Grela, E R


    An influence of various humic-plant feed additives based on some herbs (nettle, chamomile, yarrow, perforatum), lucerne and humic materials on biochemical indices of Lohmann Brown (LB) layers blood plasma was estimated. Hens were housed in deep litter system, 20 birds in a group. Four groups were formed: control (C - standard feeding), and experimental, supplemented with prepara- tions: E-1 herbal-humic, E-2 humic-herbal and E-3--humic-lucerne. Hens were placed in the pens on the 16th week of life, addition of preparations with standard food mixture started at the 22nd wk and lasted until 66th wk of life. Blood for analyses was collected four times in the following periods: 27, 37, 54 and 65th wk of life. The applied humic-plant preparations to a limited degree affected the values of examined biochemical parameters in serum: total protein (TP), albumins (Albs), glucose (Glu), urea, triacylglycerols (TAG), total cholesterol (TCh), alanine aminotransferase (ALT), aspartate aminot- ransferase (AST) and alkaline phosphatase (ALP). It is difficult to determine based on these study, which preparation is one the most active biologically, however is seems that humic-lucerne prepara- tion affected the examined blood parameters to the highest degree. The reference values ranges in hens blood serum LB hens were proposed for: TP (43-65 g/l), Albs (15-22 g/l), urea (0.5-1.2 mmol/l), Glu 10-15 mmol/l), TCh (2.2-4.5 mmol/l), TAG (10-24 mmol/l), AST (4-12 U/l), ALT (150-280 mmol/l) and ALP (190-350 U/l).

  18. Correlation Analysis between Complex Relative Permittivity and Biochemical Components for Blood of Dialysis Patients before and after Hemodialysis (United States)

    Takeda, Akira; Takata, Kazuyuki; Nagao, Hirotomo; Wang, Jianqing; Fujiwara, Osamu

    We previously measured for healthy subjects and patients who require hemodialysis the complex relative permittivity (εr´-jεr´´) of whole blood, and found that εr´-axis intercept εrt´ of straight line approximation to the Cole-Cole plots at frequencies from 200 MHz to 1GHz is significantly different with a level of less than 1% among its averaged values for healthy subjects and patients before and after dialysis, though any correlations between εrt´ and blood urea nitrogen (BUN) and creatinine being used as main indices for hemodialysis evaluation have not so far been clarified. In this study, to make correlation analyses between the intercept εrt´ and blood biochemical components for nine patients before and after hemodialysis, we measured six kinds of their blood components including BUN and creatinine, and calculated their Pearson product-moment correlation coefficients for εrt´ along with significant probability P based on a t-test. It should be noted that P is a reference probability to determine whether or not a null hypothesis can be rejected, and that the P value of 0.05 is commonly used as a significance level for statistical test. As a result, we found that a strong correlation with P 0.05 between εrt´ and other blood components including BUN and creatinine. Although albumin is not used as an index for hemodialysis efficiency, it can reflect water amount in blood vessels and liver operation. This finding implies that εrt´ could be used as an index for evaluating blood viscosity and liver function.

  19. Effects of substituting soya bean meal (SBM) with blood meal (BM) on biochemical profile of pregnant pigs. (United States)

    Abonyi, Festus Otaka; Machebe, Ndubuisi Samuel; Ezea, Michael Sunday; Eze, James I; Omeke, Benjamin Chigozie; Marire, Benjamin Nwabueze


    Twenty-four Large White × Landrace crossbreed primigravid pigs, aged 7.50 to 8.00 months weighing between 86.15 and 88.24 kg were used to study the effects of feeding graded levels of soya bean meal (SBM) replaced blood meal (BM) diets on serum biochemical profile in gestating pigs. The pigs were randomly allotted to four finisher diets formulated such that BM replaced SBM at 0.0, 50.0, 75.0 and 100.0 %, respectively. The diets were T1 (100.0 % SBM, 0.0 % BM), T2 (50.0 % SBM, 50.0 % BM), T3 (25.0 % SBM, 75.0 % BM) and T4 (0.0 % SBM, 100.0 % BM). Individual animal's daily ration of the test diets was 2.20, 2.00 and 2.50 kg at stages one, two and three of gestation. Blood sampling and analysis for the effects of the test diets on biochemical profile of the experimental animals were carried out prior to conception, at weeks 3, 7 and 11 of gestation, respectively. The result showed no significant (P ≥ 0.05) dietary treatment effects on total protein, albumin, globulin fraction, aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase, creatinine and urea profile of the pigs fed with BM diets when compared to the control fed with 100.0 % SBM. There was however a significant (P ≤ 0.05) variation in these biochemical indices in all the experimental groups at different stages of gestation. It was concluded that BM can replace 100.0 % of SBM in the diets of pregnant pigs in the tropical humid environment without any deleterious effect on their health.

  20. Investigating the Effect of Oral Consumption of Tear Assafoetida on Hepatic, Renal, Cardiac, and Blood Biochemical Parameters of Rats

    Directory of Open Access Journals (Sweden)

    M Esmailidehaj


    Methods: Forty male Wistar rats, with average weighting of 300 grams, were divided into five groups of eight in each. The first group was considered as control group and groups 2-5 as the pretreatment groups that were pre-treatmented by 12.5, 25, 50 and 100 mg/kg tear assafoetida for 30 days, respectively. Then, blood samples were collected for measurement of biochemical parameters of liver (AST, ALT, ALP, kidney (Urea, Cr, cardiac (CPK and blood coagulation (Pt, Ptt. Blood pressures were also recorded through inserting a cannula into the carotid artery. Lead II of electrocardiogram was used to monitor electrical activity of the heart. Data was analyzed by one-way analysis of variance. Results: The plasma level of AST, CPK and Ptt significantly reduced in pretreatment groups with assafoetida (P<0.05. With increasing the dose of assafoetida, plasma urea concentration reduced and plasma Cr concentration and all blood pressures increased insignificantly. Conclusion: The findings of this study suggest that low doses of tear assafoetida (25 mg/kg does not probably have any side effects and even maybe useful for health, but at higher doses (100 mg/kg it may lead to complications. Hence, there is a need for further studies to answer some questions that arose from this study.

  1. Age-Dependent Variation in Hormonal Concentration and Biochemical Constituents in Blood Plasma of Indian Native Fowl

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    Avishek Biswas


    Full Text Available This experiment was to investigate the age-related changes in hormonal concentration and biochemical constituents of blood plasma in Indian native desi fowl. One hundred and sixty two (54 from each breed, i.e., Kadaknath (KN, Aseel peela (AP, and White leghorn (WLH day-old female chicks were randomly divided into nine groups each of 18 chicks (3 groups × 3 replicates. WLH was taken in this study to compare the characteristics of Indian native desi fowl. The highest level of estrogen hormone in WLH and desi fowl in blood plasma was occurred at 18 and 24 wks of age, respectively. Whereas, the peak of progesterone hormone in WLH hens noticed around 24 wks, in case of desi fowls, it was at 30 wks of age. Irrespective of the breed, the hormonal profile of Triiodothyronine (T3 and Thyroxine (T4 in blood plasma was found highest around 6 to 12 wks of age. Activities of acid phosphatase (ACP increased with the reduction of alkaline phosphate (ALP activities at different time intervals. Irrespective of the breed, transaminases (glutamic oxaloacetic transaminase (GOT and glutamic pyruvic transaminase (GPT activities of blood plasma increased linearly with the advancement of the age. From this study, it may be concluded that sexual maturity of the Indian native desi fowl occurred nearly 6 wk later (24 wk than WLH.

  2. The in Vitro Assessment of Biochemical Factors in Hepatocyte like Cells Derived from Umbilical Cord Blood Stem Cells

    Directory of Open Access Journals (Sweden)

    A KHoramroodi


    Full Text Available Introduction & Objective: Umbilical cord blood (UCB is a source of Hematopoietic Stem Cells (HSC and progenitor cells that can reconstitute the hematopoietic system in patients with malignant and nonmalignant disorders. Mesenchymal stem cell-derived from umbilical cord blood (UCB have been differentiated to some kind of cells, such as osteobblast, adipoblast and chondroblast in Vitro. This study examined the differentiation of Umbilical Cord Blood (UCB derived stem cells to functional hepatocytes. Materials & Methods: The present study was an experimental study which was carried out in the Payam-e-Noor University of Tehran in cooperation with Hamedan University of Medical Sciences in 2008. Umbilical cord blood (UCB was obtained from Fatemieh hospital (Hamadan, Iran. Stem cells were isolated from the cord blood by combining density gradient centrifugation with plastic adherence. When the isolated cells reached 80% confluence, they differentiated to hepatocyte like cells. The medium which was used was consists of DMEM and 10% Fetal Bovine Serum (FBS supplemented with 20 ng/mL Hepatocyte Growth Factor (HGF, 10 ng/mL basic Fibroblast Growth Factor (bFGF and 20 ng/mL Oncostatin M (OSM.The medium was changed every 3 days and stored for Albumin (ALB, Alpha Fetoprotein (AFP, Alkaline Phosphatase (ALP, and urea assay. Finally PAS stain was done to study Glycogen storage in the differentiated cell. Results: Measurement of biochemical factors in different days showed that concentration of albumin (ALB, alpha fetoprotein (AFP, alkaline phosphatase (ALP, and Urea gradually increased. Also, PAS staining showed the storage of glycogen in these cells. Conclusion: Stem cell-derived from human umbilical cord blood (HUCB is a new source of cell types for cell transplantation therapy of hepatic diseases and under certain conditions these cells can differentiate into liver cells.

  3. Aliskiren in combination with losartan reduces albuminuria independent of baseline blood pressure in patients with type 2 diabetes and nephropathy

    DEFF Research Database (Denmark)

    Persson, Frederik; Lewis, Julia B; Lewis, Edmund J


    Elevated BP contributes to development and progression of proteinuria and decline in renal function in patients with type 2 diabetes. Our post hoc analysis assessed the baseline BP influence on the antiproteinuric effect in the Aliskiren in the Evaluation of Proteinuria in Diabetes (AVOID) study....

  4. Biochemical characterization of blood orange, sweet orange, lemon, bergamot and bitter orange. (United States)

    Moufida, Saïdani; Marzouk, Brahim


    This paper reports on the composition of aroma compounds and fatty acids and some physico-chemical parameters (juice percentage, acidity and total sugars) in five varieties of citrus: blood orange, sweet orange, lemon, bergamot and bitter orange. Volatile compounds and methyl esters have been analyzed by gas chromatography. Limonene is the most abundant compound of monoterpene hydrocarbons for all of the examined juices. Eighteen fatty acids have been identified in the studied citrus juices, their quantification points out that unsaturated acids predominate over the saturated ones. Mean concentration of fatty acids varies from 311.8 mg/l in blood orange juice to 678 mg/l in bitter orange juice.

  5. Morphological and biochemical characterization of mitochondria in Torpedo red blood cells. (United States)

    Pica, A; Scacco, S; Papa, F; De Nitto, E; Papa, S


    A study is presented on the morphology and respiratory functions of mitochondria from Torpedo marmorata red blood cells. In vivo staining of red blood cells and transmission electron microscopy showed the existence of a considerable number of vital and orthodox mitochondria which decreased from young erythroblasts to mature erythrocytes from 60-50 to 30-20 per cell. In erythrocytes mitochondria exhibited a canonical, functional respiratory chain. The content and activity of cytochromes in erythrocytes were, however, significantly lower as compared to mammalian tissues.

  6. Effect of exercise on Special Aviation Gymnastics Instruments on blood serum levels of selected biochemical indices in cadets

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    Zbigniew Wochyński


    Full Text Available Introduction. Aim of this study was the training effect evaluation on the Special Aviation Gymnastics Instruments (SAGI on blood metallothionein (MT, zinc (Zn, copper (Cu, protein, neuron-specific enolase (NSE, and physical fitness in the examined cadets. Material and methods. The study comprised 55 cadets, aged 20, divided into two groups: examined group A (N=41 and control group B (N=14. In both groups, blood material was collected twice, i.e. before (baseline and after training (series I, during (series II, and after completion of training on the SAGI (Series III. Blood serum MT, Zn, Cu, protein, and NSE were assayed with commercially available kits. Physical fitness was assessed with commonly used fitness tests. Results. A significant decrease in serum MT was noted in both groups in all three series of assays after training, except group B in series II. NSE significantly increased in group A in series II after training. NSE activity increased significantly in group B in series I and III. In both groups, a significant decrease in blood serum Zn was noted after training in series I and II. Serum Cu significantly decreased in group A in all three series of assays. Blood serum protein significantly decreased in group A in series III. In series II, blood serum protein increased significantly in both groups. The remaining values were not changed significantly. Conclusions. Training intensity on SAGI lowered serum MT levels after training in comparison with the control group. This might be associated with Zn, Cu, and protein metabolism.


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    Full Text Available ABSTRACT: CONTEXT: Mankind exhibits epilepsy as one of the common neurologic disorders. Standard drug therapy of this disease provides control of seizures in more than 80% of patients. Effective, specific treatment by Anti - Epileptic Drugs becomes absolutely essential for successful treatment in human subjects. Epilepsy especially Grand - Mal variant is typically treated with Phenytoin. Unique point about our study is focused on the estimat ion of serum phenytoin sodium concentration and to correlate it with some of the important biochemical markers that assess the hepatic function. AIMS: 1. It may help to assess whether the epileptic patient receiving phenytoin on an out - patient basis requir es an additional dose when monitoring shows suboptimal levels. 2. Should the maintenance dose be reduced, stopped or adjusted to minimize the dose related adverse effects without sacrifice of seizure control. 3. Study also provides an estimation of out - pat ient compliance. 4. Rational designing of dosing regimen, prediction of future drug concentration and assessment of functional impairment due to disease may be possible. 5. Failure to provide adequate control in maximal tolerated dose by phenytoin after co nfirming compliance may give a clue to substitute another drug. METHODS AND MATERIALS: Thirty seven patients suffering from grand mal epilepsy who attended the neurology OPD of Basaveshwar Teaching and General Hospital were selected for the study. Two pati ents received 100mg, 25 patients 200mg and 10 patients received 300mg of Dilantin Sodium (Parke Davis capsule daily at night between 9 - 10pm. The period of the exposure to the drug varied from 1 - 5 years. Ten healthy volunteers of same age group who were no t receiving any drugs were the control for the study. 10ml of blood was collected from each patient between 9 - 10am. 2ml of serum was extracted from each sample; serum phenytoin level was measured using UV Spectrophotometer, Bausch and Lomb

  8. BBB on chip: microfluidic platform to mechanically and biochemically modulate blood-brain barrier function

    NARCIS (Netherlands)

    Griep, L.M.; Wolbers, F.; Wagenaar, de B.; Braak, ter P.M.; Weksler, B.B.; Romero, A.; Couraud, P.O.; Vermes, I.; Meer, van der A.D.; Berg, van den A.


    The blood-brain barrier (BBB) is a unique feature of the human body, preserving brain homeostasis and preventing toxic substances to enter the brain. However, in various neurodegenerative diseases, the function of the BBB is disturbed. Mechanisms of the breakdown of the BBB are incompletely understo

  9. Liver function in Huntington's disease assessed by blood biochemical analyses in a clinical setting

    DEFF Research Database (Denmark)

    Nielsen, Signe Marie Borch; Vinther-Jensen, Tua; Nielsen, Jørgen E.


    , the manifest HD gene-expansion carriers displayed moderate increases in total cholesterol and blood glucose relative to the premanifest HD gene-expansion carriers, as well as increased C-reactive protein relative to HD gene-expansion negative controls. Our results show that LFT values are elevated more...

  10. Biochemical characterization of blood plasma of coronary artery disease patients by in vitro high-resolution proton NMR spectroscopy

    Indian Academy of Sciences (India)

    Anu Malik; Uma Sharma; R Lakshmy; Rajiv Narang; Naranamanglam R Jagannathan


    This study aimed to investigate the biochemical profile of blood plasma of patients with coronary artery disease (CAD) and angiographically normal subjects (controls) to determine biomarkers for their differentiation. In this double blind study, 5 mL venous blood was drawn before angiography from CAD patients (n=60) and controls (n=13) comprising angiography normal individuals. In vitro high-resolution NMR spectroscopy of these blood plasma samples was carried out at 400 MHz, and intensity data were analysed with partial least square discriminant analysis. Categorization of subjects as controls or CAD patients and the patients further as single vessel disease (SVD), double vessel disease (DVD) and triple vessel disease (TVD) was done at the end of the study based on their angiography reports. Raised levels of lipids, alanine (Ala) and isoleucine/leucine/valine (Ile/Leu/Val) were observed in CAD patients compared with controls. Partial least square discriminant analysis showed separation between controls vs CAD patients. TVD patients showed increased levels of Ile/Leu/Val and Ala compared with controls and SVD. Alanine, Ile/Leu/Val, and LDL/VLDL appear as possible biomarkers for distinguishing between controls and patients with SVD and TVD. A metabolic adaptation of myocardium may play a role in raising the Ala level.

  11. Changes in white blood pictures and some biochemical parameters of dairy cows in peripartum period and early lactation

    Directory of Open Access Journals (Sweden)

    Mirjana Joksimović Todorović


    Full Text Available The objective of this research was to determine the changes in the number of cells of white blood line and some biochemical parameters: concentration of glucose, concentration of non-esterified fatty acids (NEFA, activity of enzymes of aspartat aminotransferase (AST, alanin aminotransferase (ALT and gamma glutamyltranferase (GGT, in 17 Holstein-Friesian breed dairy cows in peripartum period and early lactation (on d 15 prepartum, on the day of parturition and on d 15, 30, and 45 postpartum. The highest values of total leukocytes count, neutrophiles, monocytes and basophiles count were recorded on the day of parturition, and then decreased, so that the minimum value established on d 45 of lactation. The lymphocytes count was the lowest on the day of parturation, while the eosinophils and basophiles counts were significantly different in observed time intervals. The concentration of glucose ranged from 3.07 mmol/L on d 15 prepartum to 2.71 mmol/L on d 15 postpartum. A high concentration of non-esterified fatty acids (NEFA in the postpartum period is a consequence of changes in energy balance and more intensive process of lypolisis in fat tissue, due to energy disbalance at the beginning of lactation. Established activity of enzymes AST, ALT and GGT was approximately the same in studied time intervals and was within physiological limits. The results of hematological and biochemical analysis in this paper do not indicate the development of postpartum ailments, because the investigated parameters were within physiological limits.

  12. [Blood biochemical changes in maternally deprived children and their emotional status]. (United States)

    Gogberashvili, K Ia; Gagoshidze, M V; Pagava, K I


    Environmental rearing conditions during the neonatal period are critical for the establishment of neurobiological factors controlling behavior and stress responsiveness. Early maternal deprivation in animals consisting of a single 24-h maternal deprivation episode during early neonatal life has been proposed as an animal model for certain psychopathologies including anxiety, depression and schizophrenic-related disorders. The aim of the present research was to show the mechanisms how the early maternal deprivation in humans influences the emotional status of children. To understand the effects of early deprivation on the regulation of the hypothalamic-pituitary-adrenal axis of children following social interactions, we examined the blood neurotransmitters levels (Norepinefrin NA; Dofamin DF; Serotonin SE) in a group of healthy institutionalized children at age from 6 to 36 months from Tbilisi Infant's House. A group of healthy children of the same age from Mother & child shelters formed the control group. Emotional status was assessed by Leusher's Color Test in a group of healthy institutionalized children at age from 3 to 6 years from Tskneti Orphanage. Institutionalized children showed elevations in blood NE and decreased SE and DF concentrations in comparison with non deprived children's blood. The higher was percentage of children with high level of anxiety in the group of children from orphanage than in the children with family care. The results suggest that early maternal deprivation may contribute to long-term regulatory problems of the stress-responsive system that may be resulted in altered emotionality and behavior in deprived children.

  13. Blood biochemical profiles of thai indigenous and Simmental x Brahman crossbred cattle in the Central Thailand. (United States)

    Boonprong, S; Sribhen, C; Choothesa, A; Parvizi, N; Vajrabukka, C


    Plasma biochemical profiles were studied in 112 mature (3 to 5-year-old) healthy cattle comprised of 61 Thai indigenous and 51 Simmental x Brahman crossbred male and cyclic female cattle at Nongkwang (Central Thailand) Livestock Research and Breeding Center, Thailand. Data were analysed for the effect of breed and sex. The results showed that the plasma glucose and gamma-glutamyl transferase (GGT) in the two breeds were significantly (P < 0.05) different. Furthermore, the urea, creatinine, albumin, total protein, aspartate amino transferase (AST), alanine amino transferase (ALT) and alkaline phosphatase (ALP) levels in Thai indigenous were significantly (P < 0.01) higher than in crossbred cattle. However, creatine kinase did not significantly differ in crossbred and indigenous animals. A sex difference was found in glucose level with male Thai indigenous having significantly higher levels (P < 0.05) than the other three groups. Plasma urea concentration in male crossbred cattle was lower than in the other groups (P < 0.05). Female crossbred cattle had significantly (P < 0.05) lower plasma creatinine levels than the other animals. Furthermore, levels of albumin in male and total protein in female crossbred were the lowest (P < 0.05) among the groups. The AST, ALT, ALP and GGT levels were significantly (P < 0.05) different between male and female. Female crossbred cattle had the lowest (P < 0.05) AST and GGT levels, whereas lowest (P < 0.05) ALT and ALP concentration was determined in male individuals of these breeds.

  14. The Genetic Determinism of Biochemical Systems Polymorphous From the Blood Serum in Pigs

    Directory of Open Access Journals (Sweden)

    Nicoleta Işfan


    Full Text Available The study of genetic markers and identification of new markers make the subject of an increasing number of research projects in various fields such as genetics of immunology, biochemical genetics, molecular genetics, quantitative genetics and the genetic amelioration of animals. The information provided by electrophoresis graphs has been used to determine the frequency of various categories of alleles (for the loci of pre-albumin, transferines and serum amylases, the frequency of various phenotypes and the genetic structure for each and every locus and, simultaneously, for the loci being studied. The discussion over the varieties of serum proteins was carried on for the purpose of using them as genetic markers, in order to appreciate the levels of genetic unity or diversity within the stock of swine that has been studied. A pair of simple alleles has been determined for each of the three loci. When the three loci were studied simultaneously, out of the 27 possible combinations, only 15 have been found. The sample studied has found to be genetically balanced for every of the three loci. However, when the simultaneous study has been applied, the same sample has not been found genetically balanced.

  15. Influence of peripartum dietary energy supplementation on some blood biochemical parametars in dairy cows

    Directory of Open Access Journals (Sweden)

    Bojković-Kovačević Slavica


    Full Text Available The aim of this study was to determine the influence of an energy supplement on the basis of propylene glycol on the values of metabolic profile parameters in periparturient cows. Fifteen days before calving, 40 cows were selected and divided into two groups: control (n=20 and experimental (n=20. From day 15 before to day 30 after calving, the experimental group of cows received an energy supplement based on propyleneglycol added in food ("OSIMOL“, Veyx-Pharma GmbH, Germany. Blood samples were taken 15 days before and 10 days after calving. Concentrations of glucose and betahydroxybutyric acid (BHBA were determined immediately after blood was taken. Concentrations of total protein, albumin, urea and total bilirubin were determined in the blood serum. There was no significant difference in the average values of the measured parameters between the control and the experimental group of cows. After parturition glycemia in cows of the control group was lower than physiologically acceptable (x=1.93±0.43 mmol/L, and glucose in cows of the experimental group was at the upper physiological limit (x=3.13±0.33 mmol/L and signicantly higher (p<0.001 than in the control group. The concentration of BHBA in cows that received the energy supplement („OSIMOL“ was 0.40±0.12 mmol/L, while in the control group of cows it was significantly higher (p<0.001 and stood at 0.88 ±0.39 mmol/L. Furthermore, the concentration of total bilirubin in the cows of the experimental group was within the physiological range (x = 4.09± 1.42 μmol/L, while in the cows of the control group it was significantly higher (p<0.05 and stood at 10.19±5.16 μmol/l.

  16. Interaction of lipophorin with Rhodnius prolixus oocytes: biochemical properties and the importance of blood feeding

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    Petter Franco Entringer


    Full Text Available Lipophorin (Lp is the main haemolymphatic lipoprotein in insects and transports lipids between different organs. In adult females, lipophorin delivers lipids to growing oocytes. In this study, the interaction of this lipoprotein with the ovaries of Rhodnius prolixus was characterised using an oocyte membrane preparation and purified radiolabelled Lp (125I-Lp. Lp-specific binding to the oocyte membrane reached equilibrium after 40-60 min and when 125I-Lp was incubated with increasing amounts of membrane protein, corresponding increases in Lp binding were observed. The specific binding of Lp to the membrane preparation was a saturable process, with a Kdof 7.1 ± 0.9 x 10-8M and a maximal binding capacity of 430 ± 40 ng 125I-Lp/µg of membrane protein. The binding was calcium independent and pH sensitive, reaching its maximum at pH 5.2-5.7. Suramin inhibited the binding interaction between Lp and the oocyte membranes, which was completely abolished at 0.5 mM suramin. The oocyte membrane preparation from R. prolixus also showed binding to Lp from Manduca sexta. When Lp was fluorescently labelled and injected into vitellogenic females, the level of Lp-oocyte binding was much higher in females that were fed whole blood than in those fed blood plasma.

  17. Loss of blood group A in acute leukemia. Morphologic and biochemical studies of red cells. (United States)

    Atkinson, J B; Tanley, P C; Wallas, C H


    A patient with blood type A had acute myelomonocytic leukemia; his red cells (RBCs) typed as O and his serum had anti-B. RBC membranes were isolated from the patient as well as from controls with group A and O red cells. The membranes were incubated with uridine diphosphate (UDP)-N-acetyl-D-14C galactosamine in plasma from the patient and controls with group A and O red cells. RBC membranes from the patient behaved normally in that they incorporated the terminal carbohydrate responsible for blood group A activity. Scanning electron microscopy showed that the patient's RBCs had striking morphologic changes, with marked crenation and numerous knisocytes and dacryocytes. It was concluded that loss of the A antigen in this patient was not due to an abnormality of the enzyme required to convert H substance to A substance. It was postulated that weakening of the A antigen in some patients with leukemia may be related to a steric modification associated with abnormal red cell morphology.


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    Full Text Available To determine whether a clinical examination was adequate to assess the fitness of horses in a fence course riding, and to characterize the relationship between a clinical assessment of the horse's fitness, training schedule stage and its blood biochemistry, 22 horses were monitored before (S1, during training, immediately after warming-up (S2 and after an E level fence obstacle course ride (S3. The blood samples were taken from the jugular vein in the above three mentioned phases, for the determination of total protein (g/dl, nitrogen (mg/dl, glucose (mg/dl, lactic acid (nmol/l, calcium (mg/dl, cholesterol (mg/dl and phosphorus (mg/dl. The intend of the paper is to present the obtained results as a reference study for the appropriate use by clinicians, sport horses owners and trainers in view to have a solid base in evaluation, for the adequate protection of health and welfare of the jumper horses competitors.

  19. Impact of sex and diet on hematological and blood plasma biochemical profiles and liver histology of pikeperch (Sander lucioperca (L.

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    Zakęś Zdzisław


    Full Text Available The aim of the study was to determine the impact of diet and sex on the hematological and blood plasma biochemical profiles and the liver histology of pikeperch, Sander lucioperca (L. reared in recirculating aquaculture systems (RAS (initial mean body weight (BW 1.35 kg. The proximate composition of the two commercial feeds used were (protein/lipid/nitrogen-free extracts (P/L/NFE P505/L118/NFE294 g kg−1 (group I and P471/L141/NFE290 g kg−1 (group II. Neither diet nor sex had a significant impact on final fish body weight (≈ 2.0 kg. Sex was noted to significantly impact glucose content (Glu – higher in males and cholesterol (Chol – higher in females in the blood plasma. Diet was confirmed to have a significant impact on levels of hematocrit (Ht, hemoglobin (Hb, and mean corpuscular hemoglobin (MCH, and the values of these indicators were higher in group I. Sex had a significant impact on Ht, Hb, MCH, and mean corpuscular hemoglobin concentration (MCHC, with higher values in male pikeperch. Diet and sex had significant impacts on the values of the pikeperch hepatosomatic index (HSI, hepatocyte size and that of their nuclei, and the values of the nucleocytoplasmic index (NCI.

  20. Some biochemical parameters of blood plasma of turkey-hens following administration of 1,2,4-triasole derivative. (United States)

    Krauze, M; Truchliński, J; Cendrowska-Pinkosz, M


    The present study involved 180 slaughter turkey-hens of heavy Big-6 type divided into four groups (in triplicate repetition for 15 birds). All the birds were fed with the same standard full-dose mixtures in 5-stage system. The turkey-hens of groups I, II and III were given 1,2,4-triasole derivative (3-(2-pyridil)-4-phenyl-1,2,4-triasole-5-carboxylic acid), which has antibacterial, antifungal and immunomodulating properties, in amount of 50, 75 and 100 microg per 1 dm3 of water. Group IV--control was given water without the additive. The 1,2,4-triasole derivative was given to drinking water, starting from the first day of bird's life and for the whole rearing period. The present results of biochemical analysis of blood plasma showed that addition of examined substance significantly reduced concentration of protein, glucose, triglycerides and uric acid as compared to control. It was stated that tested 1,2,4-triasole derivative elevated the level of HDL fraction percentage and alkaline phosphatase activity in blood plasma.

  1. Impact of Baseline Systolic Blood Pressure and Long-Term Outcomes in Patients with Advanced Chronic Systolic Heart Failure (Insights from the BEST Trial)



    The impact of baseline systolic blood pressure (SBP) on outcomes in advanced chronic systolic heart failure (HF) patients has not been studied using propensity-matched design. Of the 2706 Beta-Blocker Evaluation of Survival Trial (BEST) participants with chronic HF, New York Heart Association class III–IV symptoms and left ventricular ejection fraction ≤35%, 1751 had SBP ≤120 (median, 108; range, 70–120) mm Hg and 955 had SBP >120 (median, 134; range 121–192) mm Hg. Propensity scores for SBP ...

  2. The Effect of Myrtus communis Extract on Liver Enzymes and Blood Biochemical Factors in Diabetic Adult Male Rats

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    Habiballah Johari


    Full Text Available Background: The aim of this study was the effect of Myrtus communis extract on liver enzymes and blood biochemical factors in diabetic adult male rats. Materials and Methods: This study has been carried out experimentally and completely random. Seventy adult male Wistar rats were divided in 7 groups including: control which received no treatment, sham who received 2 mL of distilled water, the 1st, 2nd and 3rd experimental groups which received 0.75, 1.5 and 3 mg/kg Myrtus communis leaf extract respectively, the 4th experimental group as the diabetic control group who received streptozotocin (60 mg/kg and the 5th experimental group as the diabetic treatment group who received 3 mg/kg of extract. This experiment lasted 14 days with prescript orally. After this period, all the rats, were weighted, anesthetized and blood samples were taken from the heart centrifuged and sera were evaluated for the concentration of various factors. In addition liver were removed and sliced. Results: According to the obtained results, the plasma concentration of liver enzyme (alanine aminotransferase, alkaline phosphatase, aspartate aminotransferase, cholesterol and glucose presented a significant decrease at (p≤0.05. Whereas no significant change were seen in body weight, triglyceride, urea, albumin and total protein. Histological studies of the liver tissue showed no significant difference among various groups. Conclusion: Myrtus communis is comprise of collections of flavonoids and other various components with antioxidant and anti inflammatory properties. Thence it can effective in treatment of liver diseases and decrease of blood sugar and cholesterol in diabetes mellitus patients.

  3. Correlation between Body Condition Score, Blood Biochemical Metabolites, Milk Yield and Quality in Algerian Montbéliarde Cattle

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    Charef-Eddine Mouffok1,2,*, Toufik Madani1,2, Lounis Semara1, Nadhira Ayache1 and Amina Rahal1


    Full Text Available This study aimed to investigate the correlation between body condition score (BCS, blood biochemical metabolites, milk yield (MY and quality (Mfat in Montbéliarde cattle (31 cows reared in 5 farms of Algerian semi arid area. The BCS was measured in dry and peak of lactation (6 weeks after calving. Blood samples were taken at the time of body condition (BC measurement for determination of energy (Glucose, cholesterol, triglycerides and B-Hydroxybutyrate, nitrogen (urea and albumin and mineral (calcium metabolites. Milk yield was recorded in the 6th week of lactation (peak. A sample of milk for each cow was used to determinate milk fat, density and acidity. The results showed a significant decrease in postpartum BCS accompanied by an increase in cholesterol and B-Hydroxybutyrate (BHB concentration. The correlation analysis showed that BHB concentration in pre calving was negatively correlated with BCS (r=-0.321; P<0.05 and cholesterol (r=-0.308; P<0.05. In postpartum, BCS was negatively correlated with cholesterol (r=-0.416; P<0.05, urea (r=-0.366; P<0.05 and BHB (r=-0.487; P<0.05. However, the level of milk production decreased significantly with high glucose (r=-0.449; P<0.05 and BHB (r=-0.514; P<0.05. The fat content increased significantly with blood triglycerides (r=0.681; P<0.05 and BHB (r=0.522; P<0.05 concentration, indicating a high mobilization of body reserves used for the synthesis of milk fat. In conclusion, it can be assumed that the rate of BHB seems to be the best indicator of the nutritional status of dairy cows that determines their production level and quality.

  4. Biochemical adaptations in middle-distance runners: an assessment of blood and anthropometric parameters

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    Danila Di Majo


    Full Text Available In order to understand the mechanism underlying the physiological adaptation of purely aerobic workout, we investigated the effect of 2 months of training on nine males (17-22 year-old middle distance running agonistic athletes. Blood sample was collected in the morning to analyze: hematological parameters, lipid profile, liver function enzymes [glutamate oxaloacetate transaminase, glutamate pyruvate transaminase, gamma-glutamyl transferase (γ-GT] and skeletal and myocardial markers of muscle damage [creatin kinase (CK and creatin kinase MB (CK-MB]. Endurance training, as it implies high oxygen consumption, should increase reactive oxygen species, but it has been shown that exercise leads to increased activation of antioxidant defenses. In fact, serum levels of γ-GT enzyme and total CK were not increased. On the other hand, a statistical significant reduction of CKMB has been observed. There were not variations in hematological parameters. As far as the anthropometric value is concerned, after two months of training there was a change in weight (P<0.0001. Finally, any oxidative and biological stress was highlighted in the middle distance runners but, since this is a preliminary study, it would be of interest to replicate the study on a larger sample.

  5. Significant biochemical, biophysical and metabolic diversity in circulating human cord blood reticulocytes.

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    Benoît Malleret

    Full Text Available BACKGROUND: The transition from enucleated reticulocytes to mature normocytes is marked by substantial remodeling of the erythrocytic cytoplasm and membrane. Despite conspicuous changes, most studies describe the maturing reticulocyte as a homogenous erythropoietic cell type. While reticulocyte staging based on fluorescent RNA stains such as thiazole orange have been useful in a clinical setting; these 'sub-vital' stains may confound delicate studies on reticulocyte biology and may preclude their use in heamoparasite invasion studies. DESIGN AND METHODS: Here we use highly purified populations of reticulocytes isolated from cord blood, sorted by flow cytometry into four sequential subpopulations based on transferrin receptor (CD71 expression: CD71high, CD71medium, CD71low and CD71negative. Each of these subgroups was phenotyped in terms of their, morphology, membrane antigens, biomechanical properties and metabolomic profile. RESULTS: Superficially CD71high and CD71medium reticulocytes share a similar gross morphology (large and multilobular when compared to the smaller, smooth and increasingly concave reticulocytes as seen in the in the CD71low and CD71negativesamples. However, between each of the four sample sets we observe significant decreases in shear modulus, cytoadhesive capacity, erythroid receptor expression (CD44, CD55, CD147, CD235R, and CD242 and metabolite concentrations. Interestingly increasing amounts of boric acid was found in the mature reticulocytes. CONCLUSIONS: Reticulocyte maturation is a dynamic and continuous process, confounding efforts to rigidly classify them. Certainly this study does not offer an alternative classification strategy; instead we used a nondestructive sampling method to examine key phenotypic changes of in reticulocytes. Our study emphasizes a need to focus greater attention on reticulocyte biology.

  6. Black pepper (Piper nigrum in diets for laying hens on performance, egg quality and blood biochemical parameters

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    Ramon Duque Melo


    Full Text Available The objective of this study was to evaluate the increasing levels (0, 0.1, 0.2, 0.3, 0.4, 0.5 and 0.6% of black pepper in diets for laying hens on performance, egg quality and blood biochemical parameters. Hissex White hens (n=168 at 30 weeks of age were used. The experimental method was completely randomized with seven treatments with four replicates of six birds each. Estimates of black pepper levels were determined by polynomial regression. The performance showed no significant differences (p > 0.05. The eggshell percentage was significantly influenced (p < 0.05, in which the level of 0.30% inclusion impaired eggshell quality. Triglycerides level increased significantly (p < 0.05, according to increasing levels of black pepper in the diet. It can be concluded that black pepper can be used in diets for laying hens as phytogenic additive without harming the performance. However, this inclusion causes a reduction in eggshell percentage and an increase in the level of triglycerides in the bloodstream.

  7. 浅析血液生化检测各阶段质控对策%Analysis of the various stages of quality control blood biochemical detection countermeasures

    Institute of Scientific and Technical Information of China (English)

    王光让; 李婷; 刘宁


    Objective To investigate the effects of the various stages of quality control sample pass rate of biochemical tests of blood. Methods Patients in February 2013 -2014 February 422 routine blood biochemical examination of the hospital for the study. order by patients admitted to hospital will be divided into a control group (n = 211) and experimental group (n = 211). The control group received routine blood chemistry testing. Experimental group at all stages of biochemical strict quality control testing. Compare two samples of blood biochemical test pass rate case. Results The control group of blood samples biochemical detection rate of 84.36% passing (178/211), the experimental group 98.58% (208/211), blood biochemistry experimental group was significantly higher pass rate specimens (P <0.01). Conclusion The various stages of quality control can significantly improve the pass rate of clinical specimens of blood biochemical tests provide a reliable basis for clinical diagnosis and treatment.%目的:探讨各阶段质控对血液生化检测标本合格率的影响。方法选取我院2013年2月至2013年8月422例行血生化检查的患者作为研究对象。按患者入院顺序,将其分为对照组(n=211)和实验组(n=211)。对照组接受常规血生化检测。实验组对血生化检测的各个阶段进行严格质控。对比两组血生化检测标本合格率情况。结果对照组血生化检测标本合格率84.36%(178/211),实验组98.58%(208/211),实验组血生化检测标本合格率显著高于对照组(P<0.01)。结论各阶段质控可显著提高临床血生化检测标本的合格率,为临床诊断及治疗提供可靠依据。

  8. Establishing baseline levels of trace elements in blood and skin of bottlenose dolphins in Sarasota Bay, Florida: Implications for non-invasive monitoring

    Energy Technology Data Exchange (ETDEWEB)

    Bryan, Colleen E. [National Institute of Standards and Technology, Hollings Marine Laboratory, 331 Fort Johnson Road, Charleston, South Carolina 29412 (United States); College of Charleston, Grice Marine Laboratory, 205 Fort Johnson Road, Charleston, South Carolina 29412 (United States)], E-mail:; Christopher, Steven J. [National Institute of Standards and Technology, Hollings Marine Laboratory, 331 Fort Johnson Road, Charleston, South Carolina 29412 (United States); Balmer, Brian C.; Wells, Randall S. [Chicago Zoological Society c/o Mote Marine Laboratory, 1600 Ken Thompson Parkway, Sarasota, Florida 34236 (United States)


    Several major unusual mortality events occurring in recent years have increased the level of concern for the health of bottlenose dolphin populations along the United States Atlantic and Gulf of Mexico coasts. Trace element concentrations were examined in a population of free-ranging dolphins in Sarasota Bay, Florida, in order to develop a benchmark for future comparisons within and between populations. Whole blood (n = 51) and skin (n = 40) samples were collected through capture and release health assessment events during 2002-2004. Samples were analyzed for Al, V, Cr, Mn, Cu, Zn, As, Se, Rb, Sr, Mo, Cd, and Pb by inductively coupled plasma mass spectrometry (ICPMS) and Hg via atomic fluorescence spectrometry (AFS). Trace element concentrations (wet mass) in skin were 2 to 45 times greater than blood, except Cu was approximately 1.5 times higher in blood. Statistically strong correlations (p < 0.05) were found for V, As, Se, Rb, Sr, and Hg between blood and skin demonstrating that these tissues can be used as effective non-lethal monitoring tools. The strongest correlation was established for Hg (r = 0.9689) and concentrations in both blood and skin were above the threshold at which detrimental effects are observed in other vertebrate species. Female dolphins had significantly greater Hg concentrations in blood and skin and Pb concentrations in skin, relative to males. Calves exhibited significantly lower V, As, and Hg concentrations in blood and V and Hg concentrations in skin, relative to other age classes. Rubidium and Cu concentrations in skin were greatest in subadults and calves, respectively. In blood, V, Zn, and As concentrations were significantly greater in winter, relative to summer, and the opposite trend was observed for Rb and Sr concentrations. In skin, Cu and Zn concentrations were significantly greater in winter, relative to summer, and the opposite trend was observed for Mn, Rb, Cd, and Pb concentrations. The baseline concentrations and trends

  9. Effect of coumaphos on cholinesterase activity, hematology, and biochemical blood parameters of bovines in tropical regions of Mexico. (United States)

    Pardío, Violeta T; Ibarra, Nelly De J; Waliszewski, Krzysztof N; López, Karla M


    To assess the effect of coumaphos [O-(3-chloro-4-methyl-2-oxo-2H-1-benzopyran-7-yl) O,O-diethyl phosphorothioate] exposure on physiological responses during bovine production, acetylcolinesterase (AChE) and butyrylcholinesterase (BuChE) activities were measured in whole blood, erythrocytes, and plasma of healthy male steers (Bos Taurus x Bos indicus) sprayed with coumaphos at a non-lethal dose of 1 mg kg(- 1) body weight per day once every 14 (in vivo group) or 21 days (southern and central groups). Coumaphos topically administered at 1 mg/kg body weight per day to cattle under normal management practices in tropical areas produced a significant inhibition in erythrocyte (RBC) AChE and BuAChE activities when compared to baseline levels. RBC-AChE activity for the in vivo group decreased 71.3% (P < 0.05) and BuChE activity 59.1% (P < 0.05); RBC-AChE activity decreased 55.1% (P < 0.05) (southern group) and 43.4% (P < 0.05) (central group). Compared to the control specimens, steers from in vivo, southern, and central groups after 150 days of exposure had lower (P < 0.05) leukocyte count, absolute lymphocyte, erythrocyte, and platelet counts. Decreases in RBC-AChE activities correlated with decreased lymphocyte (r = 1.000, p = 0.01), erythrocyte (r = 1.000, p = 0.003), and platelet counts (r = 0.841, p = 0.036). Significantly increased BUN levels (P < 0.05) correlated with the decrease in RBC-AChE activities (r = - 0.997, p = 0.047) and with the decrease in absolute red blood cell (r = - 0.883, p = 0.020) and lymphocyte (r = - 0.825, p = 0.043) counts; increased (P < 0.05) total plasma protein levels correlated with the decrease in RBC-AChE activities (r = -0.998, p = 0.043), absolute red blood cell (r = - 0.998, p = 0.040), lymphocyte (r = - 0.893, p = 0.017), and platelet (r = -0.855, p = 0.030) counts. The physiological responses correlated with the erythrocyte acetylcholinesterase inhibition could be considered as early indicators or warning responses of bovine

  10. Biochemical measurements on single erythroid progenitor cells shed light on the combinatorial regulation of red blood cell production. (United States)

    Wang, Weijia; Akbarian, Vahe; Audet, Julie


    Adult bone marrow (BM) erythrocyte colony-forming units (CFU-Es) are important cellular targets for the treatment of anemia and also for the manufacture of red blood cells (RBCs) ex vivo. We obtained quantitative biochemical measurements from single and small numbers of CFU-Es by isolating and analyzing c-Kit(+)CD71(high)Ter119(-) cells from adult mouse BM and this allowed us to identify two mechanisms that can be manipulated to increase RBC production. As expected, maximum RBC output was obtained when CFU-Es were stimulated with a combination of Stem Cell Factor (SCF) and Erythropoietin (EPO) mainly because SCF supports a transient CFU-E expansion and EPO promotes the survival and terminal differentiation of erythroid progenitors. However, we found that one of the main factors limiting the output in RBCs was that EPO induces a downregulation of c-Kit expression which limits the transient expansion of CFU-Es. In the presence of SCF, the EPO-mediated downregulation of c-Kit on CFU-Es is delayed but still significant. Moreover, treatment of CFU-Es with 1-Naphthyl PP1 could partially inhibit the downregulation of c-Kit induced by EPO, suggesting that this process is dependent on a Src family kinase, v-Src and/or c-Fyn. We also found that CFU-E survival and proliferation was dependent on the level of time-integrated extracellular-regulated kinase (ERK) activation in these cells, all of which could be significantly increased when SCF and EPO were combined with mouse fetal liver-derived factors. Taken together, these results suggest two novel molecular strategies to increase RBC production and regeneration.

  11. The Effect of Bioceramic Composite Extracellular Matrixes Used to Repair Bone Deficiency on Relevant Blood Biochemical Indices

    Institute of Scientific and Technical Information of China (English)


    At the base of experimental animal model construction of bone defect in New Zealand rabbit, the promoting repair effect of bioactive ceramics on bone defect as well as its machanism was studied through testing body mineral elements, enzymes related to bone morphogenetic proteins and some biochemical indexes. Refering to some documents, materials of TCP, CHA and HA were combined and TCP/BMP/ TCP-β1 and CHA/BMP/ TCP-β1, HA/BMP/ TCP-β1 composite materials were made. All kinds of them were implanted into the radial defect site of rabbit, respectively. The chosen blood indexes (Ca, P, ALP, GGT, AST, ALT, TPA, BUN and Cr) were tested by colorimetry, speed rate and bromocresol green testing methods. No abnormal effects were found in any animal after operation. Serum concentrations of Ca, P and ALP were increased with the length of time in all groups of the three kinds of composite material, mixed material and pure materials. The increases in composite material groups were more significant ( P <0.05). Comparison of the three kinds of material showed TCP > CHA > HA. There was a tendency of increased TPA and decreased BUN with the length of time. There was no significant difference between the composite material groups and pure material group (P >0.05). The three kinds of bioactive ceramics composed of extracellular matrix could increase the serum concentrations of Ca and P and activity of ALP after being implanted into defect bone and showed some repairing capacity. This provided a new area of machanism study of bone defect repair by biomaterials.

  12. The effects of blood pressure reduction and of different blood pressure-lowering regimens on major cardiovascular events according to baseline blood pressure : meta-analysis of randomized trials

    NARCIS (Netherlands)

    Czernichow, Sebastien; Zanchetti, Alberto; Turnbull, Fiona; Barzi, Federica; Ninomiya, Toshiaru; Kengne, Andre-Pascal; Lambers Heerspink, Hiddo J.; Perkovic, Vlado; Huxley, Rachel; Arima, Hisatomi; Patel, Anushka; Chalmers, John; Woodward, Mark; MacMahon, Stephen; Neal, Bruce


    Background The benefits of reducing blood pressure are well established, but there remains uncertainty about whether the magnitude of the effect varies with the initial blood pressure level. The objective was to compare the risk reductions achieved by different blood pressure-lowering regimens among

  13. The dynamics of biochemical parameters in blood of clinically healthy holstein cows from day 5 before to day 60 after calving

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    Irena Celeska


    Full Text Available The peripartal period in Holstein dairy cows is critical, due to the transition from pregnancy to lactation. We have studied the dynamics of biochemical parameters from day 5 before to day 60 after calving. The study included 10 multiparous Holstein cows, examined at days -5, 5, 10, 30 and 60 relative to calving. Blood samples were taken from vena jugularis. Analyzed biochemical parameters were glucose, triglycerides, total cholesterol, total bilirubin, albumin, total protein, urea, NEFA and BHBA. Milk production and body condition score were also estimated. Obtained results showed that cows were exposed to mild to marked metabolic distress. Energy status was changed due to increased values of NEFA and BHBA and decreased value of glucose after calving. Protein concentrations were increased at day 10 after calving, despite the decrease of the level of albumin. Urea concentrations before and after calving were within physiological range indicating an optimal protein diet. Increased values of total bilirubin at day 5 after calving indicated liver increased activity. Lipid status presented by triglycerides and total cholesterol revealed no differences in blood concentrations. Milk production was highest at day 30 after calving. BCS were highest in dry cows, thereafter they declined and recovered at day 60 after calving. In conclusion, biochemical parameters can be used as relevant indicators of metabolic distress in cows around calving with milk and BCS recording as aside parameters. Changes in some biochemical parameters indicate liver increased activity and metabolic stress, that could lead to decreased milk production, impaired reproductive performance and, finally, to illness.

  14. Detection of Babesia bovis in blood samples and its effect on the hematological and serum biochemical profile in large ruminants from Southern Punjab

    Institute of Scientific and Technical Information of China (English)

    Samreen Zulfiqar; Ali Saeed; Furhan Iqbal; Sadia Shahnawaz; Muhammad Ali; Arif Mahmood Bhutta; Shahid Iqbal; Sikandar Hayat; Shazia Qadir; Muhammad Latif; Nazia Kiran


    Objective:To determine the presence of Babesia bovis (B. bovis) in large ruminants in southern Punjab and its effect on hematological and serum biochemical profile of host animals. Methods:Blood samples were collected from 144 large ruminants, including 105 cattle and 39 buffaloes, from six districts in southern Punjab including Multan, Layyah, Muzaffar Garh, Bhakar, Bahawalnagar and Vehari. Data on the characteristics of animals and herds were collected through questionnaires. Different blood (hemoglobin, glucose) and serum (ALT, AST, LDH, cholesterol) parameters of calves and cattle were measured and compared between parasite positive and negative samples to demonstrate the effect of B. bovis on the blood and serological profile of infected animals. Results:27 out of 144 animals, from 5 out of 6 sampling districts, produced the 541-bp fragment specific for B. bovis. Age of animals (P=0.02), presence of ticks on animals (P=0.04) and presence of ticks on dogs associated with herds (P=0.5) were among the major risk factors involved in the spread of bovine babesiosis in the study area. ALT concentrations were the only serum biochemical values that significantly varied between parasite positive and negative cattle. Conclusions:This study has reported for the first time the presence of B. bovis in large ruminant and the results can lead to the prevention of babesiosis in the region to increase the livestock output.

  15. Effects of dietary chromium picolinate and peppermint essential oil on growth performance and blood biochemical parameters of broiler chicks reared under heat stress conditions (United States)

    Akbari, Mohsen; Torki, Mehran


    A study was conducted using 240 female day-old broiler chicks to evaluate the effects of dietary chromium picolinate (CrPic), peppermint essential oil (, or their combination on growth performance and blood biochemical parameters of female broiler chicks raised under heat stress conditions (HS, 23.9 to 38 °C cycling). Average daily gain (ADG), average daily feed intake (ADFI), and feed conversion ratio (FCR) were obtained from 1 to 42 days of age. Furthermore, at the end of the experiment (day 42), birds were bled to determine some blood biochemical parameters and weighed for final body weight (BW). ADFI, ADG, and BW were not influenced significantly by dietary CrPic and ( P > 0.05). A significant interaction between dietary CrPic and on FCR ( P = 0.012) was detected. FCR significantly decreased in chicks fed the diet including both CrPic and compared with the CrPic group. Significant interaction between dietary and CrPic on serum concentrations of triglycerides, glucose, and albumin were observed ( P 0.05). The serum concentrations of glucose, triglycerides were decreased ( P diet including both CrPic and Plasma chromium (Cr) content increased significantly ( P diet compared with the control group ( P < 0.05). From the results of the present experiment it can be concluded that dietary supplementation with combined and CrPic could have beneficial effects on some blood biochemical parameters of female chicks reared under heat stress conditions.

  16. Blood glucose level and other biochemical changes induced in normal mice by oral Traditional Chinese Medicine complex for diabetes (TCM-D™

    Directory of Open Access Journals (Sweden)

    Donald Koh Fook Chen


    Full Text Available Background: A number of Traditional ChineseMedicine (TCM preparations are being used for thetreatment of diabetes mellitus. Some componentsof these preparations have biochemical effects otherthan those of lowering blood glucose and indeed havebeen used for other medical indications in traditionalpractice. The primary objective of the study was todetermine the effect of the oral mixture of TraditionalChinese Medicine for diabetes (TCM-D™ complex onblood glucose level and the biochemical changes if any,on the liver (ALT, AST, gamma-GT, albumin, globulinand renal (blood creatinine, urea functions in normalmice. The oral mixture is an aqueous extract of four wellknowntraditional Chinese medicinal herbs and consistsof Trichosanthes kirilowii Maxim., Paeonia lactiflora Pall.,Glycyrrhiza uranlensis Fisch., and Panax ginseng (redCA Meyer in the proportion of 36%, 28%, 18%, and18% respectively of the dry weight. These herbs havebeen shown to have blood glucose lowering activity andhave been used for other traditional medicinal purposes.The safety of the combination was evaluated in thepresent study.Methods: Experimental Balb/c mice were treated orallyvia gastric tube with the extract at daily doses equivalentto 1 and 10 times the recommended human dose for8 weeks. Blood glucose and other biochemical profileswere monitored at pre-treatment and monthly posttreatmentuntil killed.Results: When compared to pre-treatment levels, theblood glucose levels were significantly lower in treatedanimals compared to those in the control group. Atthe recommended TCM-D™ dose the levels in treatedanimals were significantly lower than that of controlanimals and at pre-treatment. When compared withpre-treatment, the glucose levels were lowest at Week8 of treatment, the mean levels being 111.23%, 83.32%and 70.33% in control, and in animals given 1 x and10 x the recommended TCM-D™ dosage respectively.The blood glucose lowering effect was also associatedwith a significant

  17. Evaluation of changes in blood hematological and biochemical parameters in response to Islamic fasting and regular physical activity in male and female subjects

    Directory of Open Access Journals (Sweden)

    Seyyed Reza Attarzadeh Hosseini


    Full Text Available Introduction: Fasting during Ramadan is a religious obligation for healthy adult Muslims. This study aimed to investigate the effects of Ramadan fasting and physical activity on blood hematological and biochemical parameters in male and female subjects. Methods: In this study, 50 healthy subjects were randomly assigned to four groups: male control fasting (MCF group (n=13, male training fasting (MTF group (n=13, female control fasting (FCF group (n=12, and female training fasting (FTF group (n=12, and were compared in two stages (before and after the intervention. For inter- and intra-group comparisons, a repeated measure ANOVA was applied. Results: The results showed a significant decline in body weight, body mass index, body fat percentage, and waist-to-hip ratio after Ramadan fasting, compared to the pre-Ramadan period. Also, significant changes were observed in red blood cell count and hematocrit level (P

  18. Effect of tocopherol on biochemical blood parameters in pleuritis-induced rats treated with 2,3,7,8-tetrachlorodibenzo-p-dioxin. (United States)

    Całkosiński, Ireneusz; Rosińczuk-Tonderys, Joanna; Bronowicka-Szydełko, Agnieszka; Dzierzba, Katarzyna; Bazan, Justyna; Dobrzyński, Maciej; Majda, Jacek; Gamian, Andrzej


    The aim of this study was to evaluate the effect of tocopherol on pleuritis-induced rats exposed to 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD). Rats were treated with a single TCDD dose of 5 μg/kg body weight (b.w.) and then for 3 weeks they were daily supplemented with tocopherol at a dose of 30 mg/kg b.w. The inflammation was initiated by intrapleural injection of a single dose of 1% carrageenin solution in a volume of 0.15 ml. Changes in biochemical blood parameters were measured three times at the 24th, 72nd and 120th hour of pleuritis and the blood was collected from 20 animals of each group of rats (group with the control inflammation; group treated with TCDD and with control inflammation; group treated with TCDD, supplemented with tocopherol and with the inflammation). The following biochemical parameters were measured: tumor necrosis factor (TNF), interleukin-1 (IL-1), IL-2, IL-4, IL-6, procollagen, telopeptide, fibrinogen, cholesterol, urea, creatinine, aspartate aminotransferase (AspAT) and alanine aminotransferase (AlAT). Daily supplementation of tocopherol caused significant changes in the level of TNF, IL-1, IL-4, IL-6, urea, creatinine, AspAT and AlAT. According to the results of these studies, we suggest that tocopherol supplementation in high doses could act as a protective treatment to improve liver metabolism.

  19. Blood biochemical changes in lambs infected with normal and gamma irradiated third stage larvae of Dictyocaulus filaria

    Energy Technology Data Exchange (ETDEWEB)

    Bhat, T.K.; Dhar, D.N.; Bansal, G.C.; Sharma, R.L. (Indian Veterinary Research Inst., Srinagar (India). Regional Centre)


    Primary infections with normal third stage larvae of Dictyocaulus filaria at a dose of 150 1/kg caused significant decrease in the levels of haemoglobin, blood glucose, serum total proteins, serum albumin, albumin/globulin ratio and increase in levels of total globulins and lactate dehydrogenase (LDH) activity in lambs. Almost similar changes in the above blood constituents excepting for haemoglobin, blood glucose and LDH activity were noticed in lambs immunised with two doses of gamma irradiation larvae and subsequently challenged with normal larvae of D. filaria at a dose of 150 1/kg. In both the infected groups, serum calcium, inorganic phosphorus, malate dehydrogenase and alkaline phosphatase activities were, however, not affected.

  20. Effect of ensiled mulberry leaves and sun-dried mulberry fruit pomace on finishing steer growth performance, blood biochemical parameters, and carcass characteristics.

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    Zhenming Zhou

    Full Text Available Fifty-one Simmental crossbred steers (357.0 ± 16.5 kg were used to compare a standard total mix ration (TMR with variants on animal performance, ruminal fermentation, blood biochemical parameters, and carcass characteristics. Corn grain and cotton seed meal were partially replaced by ensiled mulberry leaves (EML or sun-dried mulberry fruit pomace (SMFP. Experimental diets had similar amounts of crude protein (CP, acid detergent fiber (ADF, and metabolizable energy (ME. Animals were divided into three groups: control group (CONT, 8% EML group, and 6.3% SMFP group. Performance, including average daily weight gain (ADG, and dry matter intake (DMI, was measured. Blood and rumen samples were collected at the end of the experiment (16 weeks. There were no differences in final body weight (P = 0.743, ADG (P = 0.425, DMI (P = 0.642, or ADG/DMI (P = 0.236 between the groups. There were no differences (P = 0.2024 in rumen pH values; ammonia N was lower (P = 0.0076 in SMFP than in the EML and CONT groups. There were differences in the concentrations of total and individual volatile fatty acids, while no differences were determined in blood biochemical parameters (i.e., plasma glucose, urea concentrations, triglycerides, total protein, insulin, IgG, alanine transaminase, and aspartate aminotransferase, P ≥ 0.098. No differences were observed in carcass characteristics (P ≥ 0.513, tenderness (P = 0.844, adipose and lean color values (P ≥ 0.149, and chemical composition (P ≥ 0.400; however, intramuscular fat was lower in the EML and SMFP groups compared to the CONT animals (P = 0.034. In conclusion, diets supplemented with these two mulberry products in an isocaloric and isonitrogenous manner have similar effects to corn grain and cotton seed meals on steer performance, blood biochemical parameters and carcass characteristics, with the exception of ruminal VFA concentrations and lower intramuscular fat content.

  1. Association between the biochemical profiles in blood and bone mineral density in Chinese Han population: findings from a cross-sectional study

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    Chen HX


    Full Text Available Hong-Xia Chen,1,2,* Li-Bing Wu,3,* Zhong-Ji Meng1 1Institute of Biomedicine, Taihe Hospital, Hubei University of Medicine, Shiyan, Hubei Province, People’s Republic of China; 2Department of Environmental, Agricultural, & Occupational Health, College of Public Health, University of Nebraska Medical Center, Omaha, NE, USA; 3Department of Nuclear Medicine, Taihe Hospital, Hubei University of Medicine, Shiyan, Hubei Province, People’s Republic of China *These authors contributed equally to this work. Abstract: This study investigated the possible correlation between blood biochemical profiles and bone mineral density (BMD in the Chinese Han population. We conducted a cross-sectional analysis using participants randomly selected from the Health Screening Center of Taihe Hospital, Hubei University of Medicine, which included a total of 285 individuals, varied in ages from 31 years to 63 years. The height, weight, and body mass index (BMI of each subject were measured, as well as fasting blood glucose (FBG, triglycerides, total cholesterol, blood uric acid (UA, blood urea nitrogen, and blood creatinine. We evaluated BMD at the wrist (grams per centimeter square using a dual-energy X-ray absorptiometry scan. The mean ages of female and male groups were 45.32±8.24 years and 46.76±9.01 years, respectively. The mean age of the study population was 46.14±8.79 years, which is similar to the general population. The mean BMI values were 22.08±2.38 kg/m2 and 24.50±2.67 kg/m2 in the female and male population, respectively, representing a statistically significant difference (P<0.05. No significant differences in levels of FBG total cholesterol, or triglycerides were seen among the male and female population. Multiple linear regression analysis was performed using BMD as outcome variables and BMI, blood UA, FBG, total cholesterol, triglycerides, urea nitrogen, and blood creatinine as covariates, and the regression equation was established as Y=0

  2. The Relationship Between Baseline Blood Pressure and Computed Tomography Findings in Acute Stroke Data From the Tinzaparin in Acute Ischaemic Stroke Trial (TAIST)

    DEFF Research Database (Denmark)

    Sare, G.M.; Bath, P.M.W.; Gray, L.J.;


    .06-1.17) at baseline, and signs of visible infarction at day 10 (OR, 1.06; 95% CI, 1.00-1.13). A lower SBP was associated with signs of acute infarction (OR, 0.94; 95% CI, 0.89-0.99). Hemorrhagic transformation, dense middle cerebral artery sign, mass effect, and cerebral edema at day 10 were not independently...... associated with baseline BP. Conclusion-Although high baseline BP is independently associated with a poor outcome after stroke, this was not shown to be through an association with increased hemorrhagic transformation, cerebral edema, or mass effect; trial design may be suboptimal to detect this. Higher SBP...

  3. 全自动生化仪与快速血糖仪测定血糖浓度的比较%Comparison between automatic biochemical analyzer and rapid blood sugar meter for determining glucose blood concen-tration

    Institute of Scientific and Technical Information of China (English)

    刘兴高; 成平


    目的:探讨全自动生化仪测定静脉促凝血清标本血糖浓度与快速血糖仪测定静脉抗凝全血标本血糖浓度的相关性及差异。方法采集该院200名住院患者的血液标本,同一患者同时采集静脉促凝血清标本与静脉抗凝全血标本,采用全自动生化仪测定静脉促凝血清标本血糖浓度,同时采用快速血糖仪测定静脉抗凝全血标本血糖浓度,比较2组血糖浓度测定结果。结果191名患者的静脉促凝血清标本血糖浓度明显大于其静脉抗凝全血标本血糖浓度,差异范围在0.50~1.50 mmol/L ,差异有统计学意义(P<0.05)。结论全自动生化仪测定静脉促凝血清标本血糖浓度明显高于快速血糖仪测定静脉抗凝全血标本的血糖浓度。%Objective To investigate the correlation and difference between the automatic biochemical analyzer for determining the blood glucose concentration in the venous coagulant serum specimens and the fast blood glucose meter for determining the blood glucose concentration in the venous coagulant whole blood specimens .Methods The blood specimens of 200 inpatients in the Geleshan Branch Hospital were collected ,venous coagulant serum specimens and the venous coagulant whole blood specimens in the same patient were simultaneously collected .The automatic bi‐ochemical analyzer was adopted to detect the blood glucose concentration of venous coagulant serum specimens ,and at the same time the rapid blood glucose meter was adopted to detect the blood glucose concentration of venous coagu‐lant whole blood specimens .The blood glucose detection results were compared between the two detection methods . Results In 191 patients ,the blood glucose concentration of venous coagulant serum specimens was significantly grea‐ter than that of the venous coagulant whole blood specimens ,the difference range was 0 .50 -1 .50 mmol/L ,the difference was statistically significant (P<0 .05

  4. The Effect of Long-term Grazing Sheep in the Cotton Stubble on the Blood Biochemicals and Free Gossypol Residues

    Institute of Scientific and Technical Information of China (English)

    R.Halida; Y.Aibibula


    The objective of this study was to investigate the effect of long-term grazing sheep in cotton stubble on variation of biochemistry components and free gossypol residual in the blood. The survey found that 15% ewe of grazing group suffered miscarriage or premature birth,compared with the captive group ewes without this phenomenon. Blood test result shows that the free gossypol concentration in serum of grazing group was significantly higher than in indoor captive feeding group( P < 0. 01). In order to ensure the health of sheep grazing on cotton stubble and improve breeding rate,there is a need to do further reasonable research of grazing and feeding methods.

  5. Blood glucose test in the fast blood glucose meter and the value of conventional biochemical instrument analysis%血糖检验中快速血糖仪和常规生化仪的价值分析

    Institute of Scientific and Technical Information of China (English)

    胡政兵; 刘宁


    目的::探讨快速血糖仪和常规生化仪在临床血糖检验中应用的价值差异。方法:某院收治的82例患者为研究对象,对所有患者均采用快速血糖仪和常规生化仪检验血糖,对比两种方法的应用价值的差异。结果:快速血糖仪单次检测的血糖值为(6.20±1.18)mmol/L,多次检测的血糖值为(5.53±1.03)mmol/L,常规生化仪单次检测的血糖值为(5.69±1.12)mmol/L,多次检测的血糖值为(5.81±0.96)mmol/L。经统计学分析,两种方法比较差异无统计学意义(P>0.05)。结论:快速血糖仪具有常规生化仪在检验血糖方面的准确性,而且使用更简易可行和经济实用,能一定程度上减轻患者的身心负担和经济压力,利于医生及时确诊和给予救治措施,具有一定的临床推广和应用价值。%Objective:To study the fast blood glucose meter and conventional biochemical instrument in clinical application value of differences in blood sugar test. Methods: 82 cases of patients as the research object, adopt the fast blood glucose meter for all patients and normal biochemical analyzer test blood sugar, compared the difference of the value of the application of the two methods. Results:blood sugar value of fast blood glucose meter single detection (6.20±1.18)mmol/L, repeatedly testing blood glucose value is (5.53± 1.03) mmol/L;Routine biochemistry analyzer the blood sugar value of single detection (5.69 ± 1.12) mmol/L, multiple testing blood glucose value is (5.81 ± 0.96) mmol/L.After statistical analysis, P>0.05 there was no statistically significant difference comparing the two methods. Conclusion:fast blood glucose meter with conventional biochemical analyzer in the accuracy of the test blood glucose, and more simple and feasible and economical and practical to use, and to a certain extent, reduce the patient's physical and mental burden and economic pressures, conducive to the doctor in time

  6. Investigation of the effect of tribulus terrestris extract on the main biochemical and haematological indices of the blood in guinea fowls (Numida meleagris

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    Christo CHRISTEV


    Full Text Available The aim of the present research was to investigate the effect of Bulgarian additive Vemoherb-T (dry extract of the annual plant Tribulus terrestris – L, produced by Vemo 99 Ltd Company, Sofia on main biochemical characteristics and hematological parameters of the blood in guinea fowl (Numida meleagris. An experiment was carried out with 30 Pearl-gray Guinea fowl (32 weeks old, distributed in two groups – a control and an experimental, 12 female and 3 male each. All birds were fed the same mixture for breeder guinea fowl. Vemoherb-T was supplemented to the compound feed of the experimental group in a daily dose of 10 mg/kg body weight for a period of 12 weeks. The tested product decreased significantly the levels of total triglycerides (P < 0.05, total cholesterol (P <0.01 and glucose (P < 0.01; P < 0.001 in male and female birds respectively in the blood serum. It was established significantly higher total protein- ((P < 0.001 and calcium (P<0.01 values in the blood serum of the treated birds. The addition of Vemoherb-T increased significantly hemoglobin level, the number of erythrocytes and leukocytes and decreased the number of eosinophils in guinea fowl from the both sexes.

  7. Interactive effects of photoperiod and light intensity on blood physiological and biochemical reactions of broilers grown to heavy weights. (United States)

    Olanrewaju, H A; Purswell, J L; Collier, S D; Branton, S L


    The effects of photoperiod, light intensity, and their interaction on blood acid-base balance, metabolites, and electrolytes in broiler chickens under environmentally controlled conditions were examined in 2 trials. A 3 × 3 factorial experiment in a randomized complete block design was used in this study. In each trial, all treatment groups were provided 23L:1D with 20 lx of intensity from placement to 7 d, and then subjected to the treatments. The 9 treatments consisted of 3 photoperiods [long/continuous (23L:1D) from d 8 to 56, regular/intermittent (2L:2D), and short/nonintermittent (8L:16D) from d 8 to 48 and 23L:1D from d 49 to 56, respectively] and exposure to 3 light intensities (10, 5.0, and 0.5 lx) from d 8 through d 56 at 50% RH. Feed and water were provided ad libitum. Venous blood samples were collected on d 7, 14, 28, 42, and 56. Main effects indicated that short/nonintermittent photoperiod significantly (P light intensity, or their interaction. There was no effect of light intensity on most of the blood variables examined. Acid-base regulation during photoperiod and light intensity exposure did not deteriorate despite a lower pH and higher partial pressure of CO2 with normal HCO3(-). These results indicate that continuous exposure of broiler chickens to varying light intensities had a minor effect on blood physiological variables, whereas the short photoperiod markedly affected most blood physiological variables without inducing physiological stress in broilers.

  8. Biochemical identification of the bovine blood group M' antigen as a major histocompatibility complex class I-like molecule

    DEFF Research Database (Denmark)

    Hønberg, L S; Larsen, B; Koch, C;


    . To elucidate the structural relationship between BoLA-A16 and blood group antigen M', immunoprecipitation experiments on red and white cell lysates isolated from M'-A16 positive and negative cattle were carried out. These results showed that M(r) 44,000 and M(r) 12000 polypeptides can be precipitated from both......Absorption and elution experiments showed that it was impossible to separate antibodies against blood group factor M' from antibodies against bovine lymphocyte antigen (BoLA) A16 in an antiserum showing haemolytic activity against M' as well as lymphocytotoxic activity against BoLA-A16...... red and white cells isolated from M'-A16 positive animals, whereas no bands were seen in M'-A16 negative animals in precipitations with the same antibody. Precipitation with a crossreacting human beta 2-microglobulin (beta 2-m) specific antibody confirmed a class-I-like structure associated with beta...

  9. Blood pressure, renal biochemical parameters and histopathology in an original rat model of essential hypertension (SHRSP/Kpo strain). (United States)

    Kato, Takashi; Mizuguchi, Nobuyuki; Ito, Akihiko


    Hypertensive nephropathy, a consequence of chronic high blood pressure, is increasingly a cause of end-stage renal diseases and its correct management is very important for clinical outcome. Spontaneously hypertensive rat (SHR/Kpo) and stroke-prone SHR (SHRSP/Kpo) strains represent models of human essential hypertension. However, the kidney injuries in SHR/Kpo and SHRSP/Kpo are not well defined. We therefore characterized the renal pathophysiology of SHR/Kpo and SHRSP/Kpo compared with normotensive control (WKY/Kpo) rats. The SHRSP/Kpo exhibited increased systolic blood pressure at 10 weeks of age, and proteinuria and increased blood urea nitrogen (BUN) and serum creatinine levels at 20 weeks. We simultaneously detected mononuclear cell infiltration, tubular injuries, accumulation of extracellular matrix and marked expression of α-SMA in the tubulointerstitium. Additionally, TGF-β1 and CTGF were up-regulated in the kidney of SHRSP/Kpo. We lastly focused on changes in glomerular cells of SHRSP/Kpo. Nestin, a podocyte marker, was detected but decreased slightly in 20-week-old SHRSP/Kpo. PECAM-1 expression was increased in SHRSP/Kpo glomeruli, indicating the thickening of glomerular endothelial cells. Moreover, we found that α-SMA, a myofibroblast marker, was also upregulated in the glomeruli of SHRSP/Kpo at 20 weeks. These findings suggest that SHRSP/Kpo could be a valuable animal model for human hypertensive nephropathy.

  10. Bisphenol A and its analogs induce morphological and biochemical alterations in human peripheral blood mononuclear cells (in vitro study). (United States)

    Michałowicz, Jaromir; Mokra, Katarzyna; Bąk, Agata


    Few studies have addressed the cellular effects of bisphenol S (BPS) and bisphenol AF (BPAF) on cells, and no study has been conducted to analyze the mechanism of action of bisphenols in blood cells. In this study, the effect of bisphenol A (BPA), bisphenol F (BPF), BPS and BPAF on human peripheral blood mononuclear cells (PBMCs) was analyzed. It was shown that BPA, BPF and BPAF in particular, decreased cell viability, which was associated with depletion of intracellular ATP level and alterations in PBMCs size and granulation. Bisphenols enhanced ROS (including OH˙) formation, which led to damage to lipids and proteins in PBMCs. The most significant alterations in ROS level were induced by BPF, and particularly BPAF. Moreover, it was shown that BPAF most strongly provoked lipid peroxidation, while BPA and BPS caused the greatest damage to proteins. It may be concluded that BPA and its analogs were capable of inducing oxidative stress and damage in PBMCs in the concentrations ranging from 0.06 to 0.5 μM (0.02-0.1 μg/ml), which may be present in human blood as a result of environmental exposure. Although, most of bisphenols studied decreased cell viability, size and ATP level at higher concentrations, BPAF exhibited its cytotoxic potential at low concentrations ranging from 0.3 to 3 μM (0.1-1.0 μg/ml) that may correspond to concentrations in humans following occupational exposure.

  11. High-Moisture Diet for Laboratory Rats: Complete Blood Counts, Serum Biochemical Values, and Intestinal Enzyme Activity (United States)

    Battles, August H.; Knapka, Joseph T.; Stevens, Bruce R.; Lewis, Laura; Lang, Marie T.; Gruendel, Douglas J.


    Rats were fed an irradiated high-moisture diet (KSC-25) with or without access to a water bottle. Physiologic values were compared between these two groups and a group of rats fed a purified diet. Hematologic and serum biochemical values, urine specific gravity, and intestinal enzyme activities were determined from samples collected from the three groups of rats. Sprague Dawley rats (n=32) fed the irradiated high-moisture diet with or without a water bottle were the test animals. Rats (n=16) fed an irradiated purified diet and water provided via a water bottle were the control group. The purified diet formulation, modified AIN-76A, is a commonly used purified diet for laboratory rodents. All rats remained alert and healthy throughout the study. A comparison of the physiologic values of rats in this study with reported normal values indicated that all of the rats in the study were in good health. Significant differences (P less than 0.05) of the physiologic values from each rat group are reported.

  12. The effect of Prosopis farcta beans extract on blood biochemical parameters in streptozotocin-induced diabetic male rats (United States)

    Dashtban, Mohsen; Sarir, Hadi; Omidi, Arash


    Background: The use of herbals in the treatment of diabetes mellitus is a well-established practice in traditional medicine. The medicinal plant Prosopis farcta has some antioxidant activity, which may be useful in diabetic patients. Since, there is no report on the antidiabetic effect of the P. farcta, this study evaluated antidiabetic activity of P. farcta bean extract (PFE) in streptozotocin (STZ)-induced diabetic rats. Materials and Methods: Hyperglycemia was induced in male albino Wistar rats by intraperitoneal injection of STZ (55 mg/kg body weight [BW]), after which, the animals were randomly allocated into six experimental groups as follows: Group 1: Normal rats (received normal saline), Groups 2 and 3: Normal rats received PFE; (50 and 75 mg/kg BW), Group 4: Diabetic control rats, Group 5: Diabetic rats received PFE (50 mg/kg BW), Group 6: Diabetic rats received PFE (75 mg/kg BW). Three days after induction of diabetes, rats were received an extract of PFE orally for 12 days. Blood samples were collected by cardiac puncture to determine liver enzymes; aspartate aminotransferase and alanine aminotransferase (AST and ALT), cholesterol, triglyceride (TG), high and low density lipoproteins (HDL and LDL). Results: The administration of PFE (50 and 75 mg/kg) in STZ-induced diabetic rats significantly reduced the blood glucose levels when compared with the STZ-control group (227.2 ± 12.00 and 259.6 ± 7.03 vs. 454.6 ± 12.66, P < 0.001). PFE in diabetic groups had no significant effect on the levels of cholesterol, TG, HDL, LDL, AST, and ALT compare to the STZ-control group. Conclusion: P. farcta could reduce blood glucose in diabetic rats. PMID:27512685

  13. Comparison of the effect of combined relaxation and music therapy on blood biochemical characters and blood pressure on type 2 diabetic patients

    Directory of Open Access Journals (Sweden)

    Ali R. Bakhshipoor


    Full Text Available Background: Undoubtedly, diabetes and its complications is one of the greatest diseases in worldwide with considerable concern for medical knowledge. There are different kinds of psychological treatments which may reduce the signs and symptoms of diabetic complications. Majority of researches indicated that consulting, music therapy and relaxation play significant and effective role on diabetes. Materials and Method: In this semi-experimental study 75 patients with diabetes type 2, according to subjects’ characters, were invited to participate in the study. They were divided into the intervention and control group randomly. The intervention group was stratified into 6 groups with 5 patients in each group. The intervention programs were carried through 10 sessions of music and relaxation therapy (consist of imagery, progressive muscle and breathing relaxation and home practice for three months. Blood pressure, total cholesterol, triglyceride, HbA1c and fasting blood glucose were measured before and after of study. All patients also filled out the questionnaire related to biography and some diabetes risk factors before and after the study. Results: A total of 73 patients (intervention group=28 and control group=45 completed the 3-month study period. The result showed that there were significant difference between FBS (p=0.03, systolic (p=0.02 and diastolic blood pressure (p=0.0001 in intervention group compared to control group. Conclusion: we suggest that combined relaxation and music therapy is a feasible and effective treatment on diabetes. Therefore, adding relaxation and music therapy to other treatments such as physical activity and diet, in people with type 2 diabetes, may help them to control and mange their diabetes

  14. Effects of medium-chain fatty acids on performance, carcass characteristics, blood biochemical parameters and immune response in Japanese quail. (United States)

    Saeidi, E; Shokrollahi, B; Karimi, K; Amiri-Andi, M


    This study had the aim of evaluating the effects of medium-chain fatty acids (MCFA) on performance, carcass characteristics, some blood parameters and antibody titre against sheep red blood cells (SRBC) in quail. A total of 240 quail chicks were allotted to 4 treatments consisting of respectively 0, 1, 2 and 4 g/kg dietary MCFA. There were no significant differences in body weight, feed intake and feed conversion ratio among treatments at different stages of the experiment. MCFAs had no significant effect on breast, thigh, liver, spleen and bursa of Fabricius weight ratios. However, the relative weight of abdominal fat significantly decreased in quail receiving 0.2 and 0.4 MCFA as compared to other treatments. Concentrations of low-density lipoprotein-cholesterol (LDL), triglycerides and total cholesterol were decreased and high-density lipoprotein (HDL)-cholesterol was increased in quail chicks receiving MCFA compared with control quail chicks. The concentrations of SRBC antibody were not statistically different among treatments. It is concluded that MCFA significantly decreased LDL, triglycerides, cholesterol and abdominal fat and increased HDL in quail chicks.

  15. Blood haematological and biochemical parameters in normal cycling, pregnant and repeat breeding buffaloes (Bubalus bubalis) maintained in isothermic and isonutritional conditions

    Institute of Scientific and Technical Information of China (English)

    Anthony Sabasthin; Venkataswamy Girish Kumar; Sumanta Nandi


    Objective:The present study was envisaged to examine the hematological and biochemical, parameters in three different groups of buffaloes (regularly cycling, pregnant and repeat breeding) maintained in isothermic and isonutritional conditions to establish the variations in blood and/or serum components in these groups.Methods:Blood samples were analyzed for hemoglobin, PCV, TLC, neutophil, lymphocyte, eosinophil, and monocyte count, glucose, total protein, albumin, globulin, urea and cholesterol.Results:The results revealed significantly lower haemoglobin and packed cell volume value in repeat breeding when compared to pregnant and regularly cycling animals. The WBC value significantly higher in repeat breeding when compared to regularly cycling animals. The average mean values of neutrophils, lymphocyte and eosinophils revealed a no significant difference in the neutrophils, lymphocyte and eosinophils in between the group. The mean values of monocytes revealed a significantly higher value in repeat breeding animals when compared to pregnant animals. The serum glucose, total protein, cholesterol and urea levels were significantly lower in repeat breeding compared to pregnant and regularly cycling animals. The levels of albumin and globulin revealed non-significant difference among the groups.Conclusions: A significant decrease in the hemoglobin, PCV, glucose, total protein, cholesterol and urea was observed in the repeat breeding animals when compared to pregnant and regularly cycling animals.

  16. Monitoring the effects of exposure to lead and cadmium in working and living environment through standard biochemical blood parameters and liver endonucleases activity

    Directory of Open Access Journals (Sweden)

    Nikolić Ružica S.


    Full Text Available Heavy metals as pollutants in the working and living environment are a serious health and environmental problem because they are toxic, non-biodegradable, accumulate in living systems and have a long half-life in soil. Sources of lead contamination are combustion products in the chemical industry and metallurgy, industrial waste water, landfills, traffic etc. Lead enters into the body via the food chain and drinking water. In the body lead is deposited in the liver, kidneys, brain and mineral tissues. Excretion of lead causes damage to the epithelial cells of certain organs. High level exposure to cadmium is usually the result of environmental pollution by human activities. Exposure to cadmium can lead to acute and chronic tissue damage of various organs, including liver and kidneys in humans and in animals. In this paper we analyzed the effects of lead and cadmium exposure, in working and living environment, on the model system of experimental animals, particularly the activity of certain liver enzymes, acid and alkaline DNase, and standard biochemical blood parameters. The study showed that lead and cadmium significantly affect the protein content, red blood cells, hemoglobin and hematocrit, and the activity of liver enzymes. This harmful effect of this toxic metal can be reduced by the supplements.

  17. Changes in blood morphology and chosen biochemical parameters in ultra-marathon runners during a 100-km run in relation to the age and speed of runners

    Directory of Open Access Journals (Sweden)

    Zbigniew Jastrzębski


    Full Text Available Objectives: The objective of the study was to reveal morphology, electrolyte and chosen biochemical parameters in terms of health risk in runners in reference to their age and running speed in the case of running a distance of 100 km, which occur after 12 h or 24 h of recovery. Material and Methods: Fourteen experienced, male, amateur, ultra-marathon runners, divided into two age and two speed groups took part in the 100-km run. Blood samples for analyses indexes were collected from the ulnar vein just before the run, after 25 km, 50 km, 75 km and 100 km, as well as 12 h and 24 h after termination of the run. Results: The sustained ultramarathon run along with the distance covered (p < 0.05 caused an increase in myoglobin (max 90-fold, bilirubin (max 2.8-fold and total antioxidant status (max 1.15-fold, which also continued during the recovery. Significant changes in the number of white blood cells were observed with each sequential course and could be associated with muscle damage. The electrolyte showed changes towards slight hyperkalemia, but no changes in natrium and calcium concentrations. There were no significant differences between the age and speed groups for all the parameters after completing the 100-km run as well as after 12 h and 24 h of recovery. Conclusions: Considering changes in blood morphology and chosen biochemical parameters in ultra-marathon runners during a 100-km run it can be stated that such an exhausting effort may be dangerous for human health due to metabolic changes and large damage to the organs. Negative metabolic changes are independent of age of an ultramarathon runner and occur both in younger (32±5.33 years and older participants (50.56±9.7 years. It can be concluded that organ damage and negative metabolic changes during a 100-km run occur similarly in participants less experienced as well as in well trained runners. Int J Occup Med Environ Health 2016;29(5:801–814

  18. HBV转基因小鼠血液生理生化指标检测分析%Hematological and biochemical indexes in blood of HBV transgenic mice

    Institute of Scientific and Technical Information of China (English)

    郑凤娇; 傅泳航; 刘光泽; 张建; 李秀梅; 陈媚娟; 孔祥平


    Objective To study the effects of gene integration of HBV on the biochemical and hematological indices in blood of transgenic mice. Methods The venous blood was collected from orbital venous plexus of 28 normal mice bom in the same brood (HBsAg negative) and 50 HBV transgenic mice (HBsAg positive), 6-8 weeks in age The blood routine examination was performed, including white blood cells (WHO, red blood cells (RBC), hemoglobin (Hb), platelets (PLT), lymphocyte percentage (L%), intermediate cell percentage (M%), the percentage of leaf cells (G%) and blood biochemical parameters including glucose (GLU), urea nitrogen (BUN), creatinine (CREA), total protein (ALB), albumin (ALB), total bilirubin (TBIL), alanine aminotransferase (ALT), aspartate aminotransferase (AST), total cholesterol (CHOL) and triglyceride (TG). The differential indexes were analyzed in HBsAg positive and negative mice, also the mice of different sex. Results Significant differences were found between the transgenic and normal mice in blood GLU, BUN, CREA, RBCs, Hb and PLT (P<0.05), while no significant differences were found in other indices. No difference was found in the above 6 indices between the different sex of normal mice, while there was significant difference in blood GLU between males and females in transgenic mice, and it was higher in males than in females ( P<0.05). The blood GLU, CREA and BUN were higher in transgenic male mice than in normal male mice, and the contents of RBCs, Hb and PLT were higher in transgenic female mice than in normal female mice (P<0.05). Conclusion HBV DNA may throw some influences on blood biochemical and hematological indexes of HBV transgenic mice, and it may provide some valuable references to the study of HBV pathogenesis in HBV animal models.%目的 研究HBV基因整合对转基因小鼠代谢和血液指标的影响.方法 以28只同一窝乙肝表面抗原(HBsAg)阴性小鼠和50只HBsAg阳性的HBV转基因小鼠为研究对象,鼠龄6~8周,眼

  19. Alterations of growth, blood biochemical components and hormone profiles by intensified nutrition in growth retarded Japanese Black cattle. (United States)

    Watanabe, Daisaku; Ikeda, Hiroki; Kazamatsuri, Hiroyuki; Ando, Takaaki; Ohtsuka, Hiromichi; Kobayashi, Shigeki; Oikawa, Masaaki; Sugimoto, Yoshikazu


    In order to determine the clinical conditions of Japanese Black (JB) cattle with growth retardation, we determined the changes of body growth, blood profiles of metabolism and hormones caused by intensified nutrition (sufficient total digestible nutrients and digestible crude protein for a target daily gain set at 1.2-1.3 kg/day) in three cattle. The daily gain (DG) was increased during the intensified period (Intense) compared with the preparation period (Pre), but the DG in the Intense period was 36-66% of the target DG. Serum albumin, total cholesterol, insulin and IGF-1 increased during the Intense period compared with the Pre period. Serum GH showed high levels in the Pre period, whereas it showed lower levels in the Intense period. These results suggested that the present growth retarded cattle had abnormalities in their metabolic systems and lacked nutrient absorption.

  20. Effects of combined dietary chromium(III) propionate complex and thiamine supplementation on insulin sensitivity, blood biochemical indices, and mineral levels in high-fructose-fed rats. (United States)

    Król, Ewelina; Krejpcio, Zbigniew; Michalak, Sławomir; Wójciak, Rafał W; Bogdański, Paweł


    Insulin resistance is the first step in glucose intolerance and the development of type 2 diabetes mellitus, thus effective prevention strategies should also include dietary interventions to enhance insulin sensitivity. Nutrients, such as microelement chromium(III) and thiamine, play regulatory roles in carbohydrate metabolism. The objective of this study was to evaluate the insulin-sensitizing potential of the combined supplementary chromium(III) propionate complex (CrProp) and thiamine in insulin resistance animal model (rats fed a high-fructose diet). The experiment was carried out on 40 nine-week-old male Wistar rats divided into five groups (eight animals each). Animals were fed ad libitum: the control diet (AIN-93 M) and high-fructose diets with and without a combination of two levels of CrProp (0.1 and 1 mg Cr/kg body mass/day) and two levels of thiamine (0.5 and 10 mg/kg body mass/day) for 8 weeks. At the end of the experiment rats were sacrificed to collect blood and internal organs for analyses of blood biochemical and hematologic indices as well as tissular microelement levels that were measured using appropriate methods. It was found that both supplementary CrProp and thiamine (given alone) have significant insulin-sensitizing and moderate blood-lipid-lowering properties, while the combined supplementation with these agents does not give synergistic effects in insulin-resistant rats. CrProp given separately increased kidney Cu and Cr levels, while thiamine alone increased hepatic Cu contents and decreased renal Zn and Cu contents.

  1. At-vessel mortality and blood biochemical status of elasmobranchs caught in an Australian commercial longline fishery

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    Paul A. Butcher


    Full Text Available This study investigates mortality of sharks in a commercial longline fishery in Australia. To examine the rate and biological, environmental and technological factors contributing to at-vessel mortality, four setlines with 120 gangions possessing ‘hook timers’ were deployed daily (for 7h and 14h using conventional gears from two commercial fishing vessels during 2013. A total of 689 animals across 22 species and including 18 elasmobranchs were landed. For the five species (Carcharhinus spp., and one genus (Sphyrna spp where there were sufficient numbers for analysis, generalised linear mixed models showed that species and the elapsed time spent on the line after hooking were the strongest predictors of at-vessel mortality, with spinner (Carcharhinus brevipinna, blacktip (C. limbatus and hammerhead (Sphyrna spp sharks exhibiting the highest death rates. The variables which best explained mortality, included: (i sex of the caught sharks, and the interaction between species with (ii capture depth, and (iii the elapsed time spent on the line after hooking. For the subset of dusky (C. obscurus and sandbar (C. plumbeus sharks examined for physiological status at the point of capture, very few of the 13 chosen blood analytes varied significantly. Given the observed high mortality rates and stress associated with the time spent on the line after capture, operational changes to reduce these adverse impacts should be considered. Even simple changes such as shorter soak times could considerably mitigate these impacts.

  2. Blood biochemical markers of bone turnover: pre-analytical and technical aspects of sample collection and handling. (United States)

    Lombardi, Giovanni; Lanteri, Patrizia; Colombini, Alessandra; Banfi, Giuseppe


    Casual or systematic errors occurring in pre-analytical, analytical or post-analytical phases influence laboratory test results. The areas where pre-analytical phase errors most often arise are: timing of specimen collection; selection of specimen type; and time and temperature of storage/transport. Bone turnover markers are clinically useful in evaluating bone metabolism. Although unquestionably valuable tools, little is known about the pre-analytical precautions for their correct use and there is no consensus on kind of sample, or storage time and temperature before analysis. Moreover, biological variability, because of uncontrollable and controllable factors, will affect pre-analytical variability. Serum should be preferred to simplify blood drawing; therefore, only one tube should be used for the analysis of all bone markers. Short-term storage at 4°C may be advisable to preserve stability, immediate storage at -70°C is recommended for longer periods, while avoiding repeated freeze-thawing cycles. Sampling should be performed in the morning in fasting subjects who have abstained from physical exercise for 24 h. This review aimed to give a knowledge update on pre-analytical phase precautions in performing bone turnover marker measurement.

  3. Haematological, biochemical and inflammatory parameters in inactive Behçet's disease. Its association with red blood cell distribution width. (United States)

    Vayá, Amparo; Rivera, Leonor; Todolí, José; Hernandez, Jose Luis; Laiz, Begoña; Ricart, José M


    Red blood cell distribution width (RDW) has been shown to be associated with disease activity in several inflammatory disorders. However only one study to show this has been conducted in patients with Behçet's disease (BD). The aim of the present study was to analyse the association of RDW with BD and its main complications; i.e.; thrombosis and posterior uveitis. A second aim was to analyse the possible correlation between RDW and both haematological and inflammatory parameters. Eighty-nine patients with BD (48 males/41 females) and 94 controls (49 males/45 females) were included in the study. Patients were in an inactive phase of the disease, showing only minimum activity. RDW was statistically higher in patients than in controls (14.02 ± 1.32 vs. 13.15 ± 0.75; p 0.05). RDW correlated negatively with haemoglobin, MCH and MCHC (p 0.05). The multivariate regression analysis revealed that haemoglobin and homocysteine were independent predictors of RDW (beta coefficient: -0.310; p = 0.003, beta coefficient: 0.379; p 14 was associated with neither thrombosis nor uveitis (p = 0.935; p = 0.553, respectively). Our results indicate that BD patients show increased RDW when compared with controls. This increase seems to be related with haematimetric indices and with homocysteine levels. Lack of correlation with inflammatory markers may be due to the fact that patients were in an inactive phase of the disease.

  4. 骨代谢相关血液生化指标的研究进展%Study on Blood Biochemical Indicators Related to Bone Metabolism

    Institute of Scientific and Technical Information of China (English)

    杜伟; 李春风; 谷大海; 徐志强; 贾俊静; 陈小波


    The bone metabolism includes the two processes of bone formation and bone resorption, which is influenced by many facters, hererity, nutrition, environment, gender and incretion are the key regulatory factors. When the bone resorption significantly exceeds formations the activity of the osteoblasts cannot keep up with the activity of the osteoclasts, and then resulting in bone loss rapidly and bone density reducing. The process of bone metabolism is affected by incretion, many biochemical indexes of blood play an important role in the study of osteopathy. Many studies have reported biochemical parameters of blood related to bone metabolism. This paper summarize the effects of serum calcium and phosphorus, parathyroid hormone, 1,25-(OH)2D3interleukin, ALP on the bone formation and resorption.%骨代谢包括骨合成和骨吸收两个过程.受到很多因素的影响,遗传、营养、环境、性别及内分泌等都是关键调节因素.当骨吸收过程大于骨合成过程,导致骨量流失,骨密度降低,进一步发展为骨质疏松症.骨代谢过程受到内分泌的影响,很多骨代谢血液生化指标对骨病的研究起到重要作用.近年来,国内外大量的研究报道了与骨代谢相关的血液生化指标.作者着重对血钙与血磷、甲状旁腺激素、1,25-二羟维生素D3、白细胞介素、血清总碱磷酶和骨碱磷酶等骨代谢血液生化指标对骨形成和分解的影响进行了综述.

  5. Effects of pollution on lead and cadmium concentration and correlation with biochemical parameters in blood of human population nearby Kosovo thermo power plants

    Directory of Open Access Journals (Sweden)

    L. Zeneli


    Full Text Available This study describes an investigation of lead and cadmium pollution of Kosovo environment as a result of outflow from the coal processing industry. In a comparative study of lead and cadmium concentration in blood of human population of two different environments in Kosovo, one nearby Kosovo Thermo Power Plants, (Obiliq a highly polluted environment and the other that is considered as relatively clean rural environment (Dragash. Analysis has shown that emission of particulate in fly ash from Thermo Power Plants during 2005 has exceeded EU standards by 400-500% and that lead concentration was 18mg kg-1 and cadmium concentration was -1 of ash. A series of determinations of lead and cadmium concentrations in blood of population that lives in this environment, have shown direct effects in biochemical parameters CRE (Creatinin, DB (Direct Bilirubine, TB (Total Bilirubine, AST (Aspartat Aminotransferaza, CK (Creatin Kinaza and CHE (Cholenisteraza in human organism. The results that were achieved in this study showed a significant difference in average lead and cadmium concentration in the blood of the investigated group of peoples that lives in the area near by the Power Plants, from a control group that lives in a rural unpolluted environment. Lead and cadmium has been analyzed in 50 samples taken from persons from industrial zone and 25 samples in controlled group. The level of lead concentration was 23.0-112.1 µg L-1in geometric average 46.05 µg L-1, cadmium concentration was 0.44-6.02 µg L-1 in geometric average of 1.56µg L-1. Controlled group from the rural relatively clean environment showed lead concentration of 6.7-33.8 µg L-1 in geometric avarage 17.76 µg L-1 and cadmium concentration of 0.21-1.8 µg L-1 or in geometric average of 0.73 µg L-1. In conclusion in exposed subjects, pollution from coal burning in Power Plant is very important factor for level of lead and cadmium concentration in blood of tested population.

  6. Effects of iron-glycine chelate on growth, carcass characteristic, liver mineral concentrations and haematological and biochemical blood parameters in broilers. (United States)

    Kwiecień, M; Samolińska, W; Bujanowicz-Haraś, B


    Studies were carried out to determine the effect of additive iron-glycine chelate on the production performance, slaughter yield, mineral deposition in the liver and the metabolic blood panel in broiler chickens. A total of 250 one-day-old Ross 308 chicks were allotted into five groups with five replicates of 10 birds each. Diets were supplemented with the organic form iron (Fe-Gly at the rate of 25%, 50% or 100% of the total requirements of the elements) and inorganic Fe (FeSO4 at the rate of 50% or 100%). In the experiment, iron was added to the premix (containing no Fe) in an amount of 40 or 20 mg per kg of basal diet, in groups I and II, in the form of FeSO4 , and in an amount of 40, 20 or 10 mg per kg of basal diet, in groups III, IV and V, in the form of Fe-Gly. The study covering the period from the first to the 42nd day of breeding revealed that the production performance and slaughter yield were not dependent on the form and amount of added Fe. In the experimental groups with the addition of Fe-Gly of 20 or 10 mg/kg, there were no deaths of chickens during the whole fattening period. As a result, introducing an organic form of iron covering 50% and 25% of the birds' requirement increased the effectiveness of chicken fattening (European Efficiency Index) (p < 0.01). An organic Fe compound (40, 20 or 10 mg/kg) added to mixtures contributed to significant changes in the level of biochemical and haematological indicators in blood. The study demonstrated that an addition of Fe-Gly to mixtures for broilers can be fully effective in terms of production and health performance even if the suggested requirement for this element is 50% or 25% covered.

  7. Selected haematological and biochemical parameters of blood in rats after subchronic administration of vanadium and/or magnesium in drinking water. (United States)

    Scibior, Agnieszka; Zaporowska, Halina; Ostrowski, Jarosław


    The purpose of these studies was to evaluate the effect of selected vanadium and magnesium doses on certain haematological and biochemical blood parameters in rats. Outbred 2-month-old, albino male Wistar rats received for a period of 6 weeks, as a sole drinking liquid, the following water solutions: group II, sodium metavanadate (SMV) at a concentration of 0.125 mg V/mL; group III, magnesium sulphate (MS) at a concentration of 0.06 mg Mg/mL; and group IV, SMV-MS solution at the same concentrations. The control group received at this time deionized water to drink. It was calculated that group II ingested with drinking water about 10.7 mg V/kg b. w./24 h, group III 6 mg Mg/kg b. w./24 h, and group IV about 9 mg V and 4.5 mg Mg/kg b. w./24 h. The exposure to vanadium alone (group II) led to a statistically significant decrease in body weight gain, food and fluid intakes. Moreover, in the same group of rats a statistically significant decrease in the RBC count, Hb concentration, MCV, and MCH values was demonstrated. Additionally, a statistically significant decrease in the plasma L-ascorbic acid concentration and a significant increase in MDA concentration in blood in this group were found. Instead, after the administration of magnesium alone (group III), a statistically significant decrease in the fluid intake and in the L-ascorbic acid concentration in plasma was noted. Furthermore, in the same group of rats a statistically significant increase in Hb level and in the plasma magnesium concentration was demonstrated. Two-way analysis of variance (ANOVA) did not reveal the interactions between V and Mg.

  8. Analysis of the routine blood counts and blood biochemical parameters and hemorheology in the rats with congenital cataract%先天性白内障大鼠血液生化及血液流变学分析

    Institute of Scientific and Technical Information of China (English)

    田小芸; 尤金炜; 董敏; 许龙祥; 赵志刚; 陈莉; 恽时锋


    Objective To measure the range of routine blood counts and blood biochemical parameters ,to analyze hemorheology of the rats with congenital cataract .Methods Blood samples were taken from 90 rats with congenital cataract weight about 185 ~211 g.Routine blood analysis was performed and blood biochemical and hemorheology paramenters were determind using an automatic blood biochemical and hemorheology analyzer .Results There were no significant difference ( P >0.05 ) between the cataract rats and the normal rats in Blood test results; but there were significant difference between the microphthalmos cataract rats and the normal same -sex rats ( P <0.01 or P <0.05 ) . The biochemical results is the cataract rats and the normal rats were different significantly in ALB group ( P <0.01 or P<0.05), and the female microphthalmos cataract rats compared with the control rats had significant difference in Ure (P<0.01) , the female cataract rats ompared with the normal rats were very significant difference in Cr group ( P <0.05 or P <0.01).The erythrocyte counts of the male cataract rats and male microphthalmos cataract rats were significantly lower than that in the female ones, respectively(P <0.05, P <0.01).The platelet counts of the male cataract rats and the male microphthalmos cataract rats were significantly higher than that in the female ones , respectively(P <0.01), and the creatinine of the male cataract rats and the male microphthalmos cataract rats were significantly lower then that in the female ones, respectively(P <0.01).There were no significant difference in every group on hemorheology .Conclusions There were significant differences in some blood indexes between the congenital cataract rats and the normal rats .These data may become useful reference for biomedical researcher in this field .%目的:测定先天性白内障大鼠血液常规、生化正常值及血液流变学变化。方法采用XS-800i血常规分析仪和OLYMPUS AV2700

  9. Studies on physiological and biochemical blood index of Girella leonina%黑鱾血液的生理生化指标研究

    Institute of Scientific and Technical Information of China (English)

    孙敏; 徐善良; 唐道军


    本文采用F-820半自动血细胞分析仪和全自动生化分析仪,对黑鱾的血液常规指标及血清中的17项生化指标进行了分析测定,并用Wright's 染色法制作血涂片对白细胞进行分类计数.结果表明:(1) 黑鱾的血红细胞数量为(2.49±0.47)×10~(12)个/dm~3;白细胞数量为(1.08±0.21)×10~(11)个/dm~3;在白细胞中以嗜中性粒细胞数量最多,占52.42%±4.67%,其次为淋巴细胞,占25.72%±2.88%;单核细胞体积最大,占总数的3.27%±0.62%.(2)黑鱾红细胞脆性大,抗性差,在0.65%~0.70%的NaCl 溶液中开始溶血,在0.50%~0.55%的NaCl溶液中完全溶血.(3)黑鱾的血红蛋白(HGB)为109.11±15.72g/dm~3,高于一般鱼类,与其较强的耐低氧生理能力相适应.(4)血清中血糖浓度为 4.72±1.35mmol/dm~3,明显低于凶猛肉食性鱼类,与其岛礁性生态习性和杂食性相吻合.(5)雌雄鱼的乳酸脱氢酶分别为332.67±188.48U/dm~3和905.66±257.85U/dm~3,雌鱼显著高于雄鱼,可能与繁殖活动相关.%The routine blood index and 17 hematic biochemical index of Girella leonine were measured by means of F820 semi-automatic hematology analyzer and automatic biochemical analyzer.The white blood cells were differential counted by Wright's staining of blood smears.The result showed that (1) the numbers of red blood cells were (2.49±0.47)×10~(12)/dm~3 and the white blood cells were(1.08±0.21)×10~(11)/dm~3 which is less than the red blood cells.In white blood cells neutrophil had the largest number and accounted for 52.42%±4.67% and the next was lymphocytes accounting for 25.72%±2.88%.The monocytes had large volume and accounted for 3.27%±0.62%.(2) The red blood cells of Girella leonine were mostly brittleness and poor resistance,and began hemolysis in 0.65%~0.70%NaCl and complete hemolysis in 0.50%~0.55%NaCl.(3) HGB was 109.11±15.72g/dm~3and was higher than that of the common fish owing to its adaptation to physiological hypoxia.(4) Glucose concentration in

  10. Blood (United States)

    ... Also, blood is either Rh-positive or Rh-negative. So if you have type A blood, it's either A positive or A negative. Which type you are is important if you need a blood transfusion. And your Rh factor could be important ...

  11. Deltamethrin-induced oxidative stress and biochemical changes in tissues and blood of catfish (Clarias gariepinus: antioxidant defense and role of alpha-tocopherol

    Directory of Open Access Journals (Sweden)

    Amin Kamal A


    Full Text Available Abstract Background The pyrethroid class of insecticides, including deltamethrin, is being used as substitutes for organochlorines and organophosphates in pest-control programs because of their low environmental persistence and toxicity. This study was aimed to investigate the impact of commonly used pesticides (deltamethrin on the blood and tissue oxidative stress level in catfish (Clarias gariepinus; in addition to the protective effect of α-tocopherol on deltamethrin induced oxidative stress. Catfish were divided into three groups, 1st control group include 20 fish divided into two tanks each one contain 10 fish, 2nd deltamethrin group, where Fish exposed to deltamethrin in a concentration (0.75 μg/l and 3rd Vitamin E group, Fish exposed to deltamethrin and vitamin E at a dose of 12 μg/l for successive 4 days. Serum, liver, kidney and Gills were collected for biochemical assays. Tissue oxidative stress biomarkers malondialdhyde (MDA and catalase activity in liver, kidney and gills tissues, serum liver enzymes (ALT and AST, serum albumin, total protein, urea and creatinine were analysed. Results Our results showed that 48 h. exposure to 0.75 μg/l deltamethrin significantly (p  Conclusions It could be concluded that deltamethrin is highly toxic to catfish even in very low concentration (0.75 μg/l. Moreover the effect of deltamethrin was pronounced in the liver of catfish in comparison with kidneys and gills. Moreover fish antioxidants and oxidative stress could be used as biomarkers for aquatic pollution, thus helping in the diagnosis of pollution. Adminstration of 12 μg/l α-tocopherol restored the quantified tissue and serum parameters, so supplementation of α-tocopherol consider an effective way to counter the toxicity of deltamethrin in the catfish.

  12. Early Rise of Blood T Follicular Helper Cell Subsets and Baseline Immunity as Predictors of Persisting Late Functional Antibody Responses to Vaccination in Humans.

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    Fabiana Spensieri

    Full Text Available CD4+ T follicular helper cells (T(FH have been identified as the T-cell subset specialized in providing help to B cells for optimal activation and production of high affinity antibody. We recently demonstrated that the expansion of peripheral blood influenza-specific CD4(+IL-21(+ICOS1(+ T helper (T(H cells, three weeks after vaccination, associated with and predicted the rise of protective neutralizing antibodies to avian H5N1. In this study, healthy adults were vaccinated with plain seasonal trivalent inactivated influenza vaccine (TIIV, MF59(®-adjuvanted TIIV (ATIIV, or saline placebo. Frequencies of circulating CD4(+ T(FH1 ICOS(+ T(FH cells and H1N1-specific CD4(+-IL-21(+ICOS(+ CXCR5(+ T(FH and CXCR5(- T(H cell subsets were determined at various time points after vaccination and were then correlated with hemagglutination inhibition (HI titers. All three CD4(+ T cell subsets expanded in response to TIIV and ATIIV, and peaked 7 days after vaccination. To demonstrate that these T(FH cell subsets correlated with functional antibody titers, we defined an alternative endpoint metric, decorrelated HI (DHI, which removed any correlation between day 28/day 168 and day 0 HI titers, to control for the effect of preexisting immunity to influenza vaccine strains. The numbers of total circulating CD4(+T(FH1 ICOS(+ cells and of H1N1-specific CD4(+IL-21(+ICOS(+ CXCR5(+, measured at day 7, were significantly associated with day 28, and day 28 and 168 DHI titers, respectively. Altogether, our results show that CD4(+ T(FH subsets may represent valuable biomarkers of vaccine-induced long-term functional immunity.

  13. Early Rise of Blood T Follicular Helper Cell Subsets and Baseline Immunity as Predictors of Persisting Late Functional Antibody Responses to Vaccination in Humans (United States)

    Borgogni, Erica; Zedda, Luisanna; Cantisani, Rocco; Chiappini, Nico; Schiavetti, Francesca; Rosa, Domenico; Castellino, Flora; Montomoli, Emanuele; Bodinham, Caroline L.; Lewis, David J.; Medini, Duccio; Bertholet, Sylvie; Del Giudice, Giuseppe


    CD4+ T follicular helper cells (TFH) have been identified as the T-cell subset specialized in providing help to B cells for optimal activation and production of high affinity antibody. We recently demonstrated that the expansion of peripheral blood influenza-specific CD4+IL-21+ICOS1+ T helper (TH) cells, three weeks after vaccination, associated with and predicted the rise of protective neutralizing antibodies to avian H5N1. In this study, healthy adults were vaccinated with plain seasonal trivalent inactivated influenza vaccine (TIIV), MF59®-adjuvanted TIIV (ATIIV), or saline placebo. Frequencies of circulating CD4+ TFH1 ICOS+ TFH cells and H1N1-specific CD4+IL-21+ICOS+ CXCR5+ TFH and CXCR5- TH cell subsets were determined at various time points after vaccination and were then correlated with hemagglutination inhibition (HI) titers. All three CD4+ T cell subsets expanded in response to TIIV and ATIIV, and peaked 7 days after vaccination. To demonstrate that these TFH cell subsets correlated with functional antibody titers, we defined an alternative endpoint metric, decorrelated HI (DHI), which removed any correlation between day 28/day 168 and day 0 HI titers, to control for the effect of preexisting immunity to influenza vaccine strains. The numbers of total circulating CD4+ TFH1 ICOS+ cells and of H1N1-specific CD4+IL-21+ICOS+ CXCR5+, measured at day 7, were significantly associated with day 28, and day 28 and 168 DHI titers, respectively. Altogether, our results show that CD4+ TFH subsets may represent valuable biomarkers of vaccine-induced long-term functional immunity. Trial Registration NCT01771367 PMID:27336786

  14. Post hoc analysis of the relationship between baseline white blood cell count and survival outcome in a randomized Phase III trial of decitabine in older patients with newly diagnosed acute myeloid leukemia

    Directory of Open Access Journals (Sweden)

    Arthur C


    Full Text Available Christopher Arthur,1 Jaroslav Cermak,2 Jacques Delaunay,3 Jirí Mayer,4 Grzegorz Mazur,5 Xavier Thomas,6 Agnieszka Wierzbowska,7 Mark M Jones,8 Erhan Berrak,8 Hagop Kantarjian9 1Department of Haematology, Royal North Shore Hospital, Sydney, NSW, Australia; 2Institute of Hematology and Blood Transfusion, Prague, Czech Republic; 3Department of Clinical Hematology, University of Nantes, Nantes, France; 4Department of Internal Medicine, Masaryk University Hospital Brno, Central European Institute of Technology, Brno, Czech Republic; 5Department of Hematology, Wroclaw Medical University, Wroclaw, Poland; 6Department of Hematology, Edouard Herriot Hospital, Lyon, France; 7Copernicus Memorial Hospital, Lodz, Poland; 8Oncology Product Creation Unit, Eisai Inc., Woodcliff Lake, NJ, USA; 9Department of Leukemia, University of Texas MD Anderson Cancer Center, Houston, TX, USA Background: In a Phase III trial, 485 patients (≥65 years with newly diagnosed acute myeloid leukemia received decitabine 20 mg/m2 intravenously for 5 days every 4 weeks or a treatment choice (supportive care or cytarabine 20 mg/m2 subcutaneously for 10 days every 4 weeks. Materials and methods: We summarized overall and progression-free survival by baseline white blood cell count using two analyses: <1, 1–5, >5×109/L; ≤10 or .10×109/L. Results: There were 446 deaths (treatment choice, n=227; decitabine, n=219. Median overall survival was 5.0 (treatment choice versus 7.7 months (decitabine; nominal P=0.037. Overall survival differences between white blood cell groups were not significant; hazard ratios (HRs favored decitabine. Significant progression-free survival differences favored decitabine for groups 1–5×109/L (P=0.005, HR =0.67, greater than 5×109/L (P=0.027, HR =0.71, and up to 10×109/L (P=0.003, HR =0.72. Conclusion: There was a trend toward improved outcome with decitabine, regardless of baseline white blood cell count. Keywords: decitabine, acute myeloid

  15. Biochemical studies of Piper betle L leaf extract on obese treated animal using 1H-NMR-based metabolomic approach of blood serum samples. (United States)

    Abdul Ghani, Zuleen Delina Fasya; Husin, Juani Mazmin; Rashid, Ahmad Hazri Ab; Shaari, Khozirah; Chik, Zamri


    Piper betle L. (PB) belongs to the Piperaceae family. The presence of a fairly large quantity of diastase in the betel leaf is deemed to play an important role in starch digestion and calls for the study of weight loss activities and metabolite profile from PB leaf extracts using metabolomics approach to be performed. PB dried leaves were extracted with 70% ethanol and the extracts were subjected to five groups of rats fed with high fat (HF) and standard diet (SD). They were then fed with the extracts in two doses and compared with a negative control group given water only according to the study protocol. The body weights and food intakes were monitored every week. At the end of the study, blood serum of the experimental animal was analysed to determine the biochemical and metabolite changes. PB treated group demonstrated inhibition of body weight gain without showing an effect on the food intake. In serum bioassay, the PB treated group (HF/PB (100mg/kg and 500mg/kg) showed an increased in glucose and cholesterol levels compared to the Standard Diet (SD/WTR) group, a decrease in LDL level and increase in HDL level when compared with High Fat Diet (HF/WTR) group. For metabolite analysis, two separation models were made to determine the metabolite changes via group activities. The best separation of PCA serum in Model 1 and 2 was achieved in principle component 1 and principle component 2. SUS-Plot model showed that HF group was characterized by high-level of glucose, glycine and alanine. Increase in the β-hydroxybutyrate level similar with SD group animals was evident in the HF/PB(500mg/kg) group. This finding suggested that the administration of 500mg/kg PB extracts leads to increase in oxidation process in the body thus maintaining the body weight and without giving an effect on the appetite even though HF was continuously consumed by the animals until the end of the studies and also a reduction in food intake, thus maintaining their body weight although they

  16. Effects of blood storage on ice in biochemical and arterial blood gas analysis of rats Efeitos da estocagem sanguínea em gelo na bioquímica e gasometria arterial de ratos

    Directory of Open Access Journals (Sweden)

    Marcus Vinicius Henriques Brito


    Full Text Available PURPOSE: To investigate the effects of blood storage in biochemical and arterial blood gas analysis of Wistar rats. METHODS: Ten adult male rats with weights between 300-350 g were used. The catheterization of the internal carotid artery were performed, followed by withdrawal of 3 ml of blood using 3 separate syringes each containing 1 ml. The syringes were fulfilled one after another and numerated according to the order of collection. Then, the following groups were devised: GT1 - samples that were firstly analyzed in 20 minutes and the second exam 65 minutes after the collection; GT2 - samples that were firstly analyzed in 35 minutes and the second exam 80 minutes after the collection; GT3 - samples that were firstly analyzed in 50 minutes and the second exam 95 minutes after the collection. The samples were stored in a container with a temperature between 0 Cº and 4 Cº. Upon analysis the values of pH, PaCO2, PaO2, HCO3-, SatO2, SBE, Na+ and K+ concentrations and glucose were compared. RESULTS: No statistically relevant difference (pOBJETIVO: Analisar o efeito da estocagem sanguínea na bioquímica e na gasometria do sangue arterial de ratos Wistar. MÉTODOS: Foram utilizados 10 ratos adultos, machos, com peso compreendido entre 300 a 350 gramas. Cada animal foi submetido à cateterização da artéria carótida seguida de heparinização prévia do animal. Foram colhidos 3 ml de sangue total do rato, separados em três seringas contendo 1 ml cada. As seringas foram preenchidas uma após a outra e enumeradas respectivamente à ordem de coleta. De acordo com esta numeração as amostras foram distribuídas nos seguintes grupos: GT1 - amostras que tiveram primeira análise em 20 minutos e a segunda análise 65 minutos após a coleta; GT2 - amostras que tiveram primeira análise em 35 minutos e segunda análise 80 minutos após a coleta; GT3 - amostras que tiveram primeira análise em 50 minutos e 95 minutos após a coleta. As amostras foram

  17. Associations between annual and seasonal variations in body mass and reproductive success and blood biochemical parameters in semi-domesticated reindeer

    Directory of Open Access Journals (Sweden)

    Ingrid H. Holmøy


    Full Text Available The main objective of the study was to follow reproductive performance and blood biochemical parameters associated with fat and carbohydrate metabolism in a herd of free-ranging, semi-domesticated reindeer (Rangifer tarandus tarandus over a two-year period, with extreme between-year variation in forage availability. The effects of climatic factors on reindeer reproductive performance were investigated by analysing whether time of onset of luteal function in autumn and calf survival were associated with changes in body mass and weather conditions, such as snow depth, precipitation, and temperature. Considerable between-year variation in the onset of luteal activity was found. In 1997, 4.2% of the female reindeer were either cycling or pregnant in the second week of October, whilst in 1998, in the same week, 100% were cycling or pregnant. Although energy balance was important for timing of the onset of luteal activity, delayed conception had no apparent effect on calf survival. The results indicated that maternal body mass (BM in spring was of primary importance for calf survival, and the productivity of the herd. Since climatic factors influence the availability of forage, and hence female BM, it also has an indirect impact on calf survival. Females with low BM demonstrated greater seasonal variation in BM than heavier females. Plasma concentrations of free fatty acids and β-hydroxy butyric acid responded to changes in forage availability, but the initial condition of the reindeer and their fat reserves also seemed to have a major influence on these parameters.Abstract in Norwegian / Sammendrag:Sammenheng mellom års- og sesongvariasjon i kroppsvekt og henholdsvis reproduksjonssuksess og biokjemiske blodparametre hos tamreinHovedformålet med studien var å følge reproduksjonen og blodparametre knyttet til fett og karbohydratmetabolismen hos en tamreinflokk (Rangifer tarandus tarandus gjennom en toårs periode med stor årlig variasjon i n

  18. Hematologic and biochemical reference intervals for wild osprey nestlings (Pandion haliaetus). (United States)

    Meredith, Anna; Surguine, Katie; Handel, Ian; Bronsvoort, Mark; Beard, Philippa; Thornton, Susan M; Wesche, Petra; Hart, Mike; Anderson, David; Dennis, Roy


    A retrospective study of blood samples from 95 osprey (Pandion haliaetus) nestlings from Scotland and England, collected opportunistically over a 10-yr period, was performed to determine hematologic and plasma biochemistry reference intervals. The age of the sampled nestlings was estimated to be between 4 and 8 wk. Ninety-five percent reference intervals were determined for all hematologic and biochemical variables using parametric and nonparametric methods as appropriate. No blood parasites were detected. This is the first published study providing baseline reference data for osprey nestlings, and it is hoped the data will be of use to wildlife veterinarians and biologists in assessing the health of this species.

  19. Feasibility Analysis of the Heparin Anticoagulant Blood Plasma Used for Emergency Biochemical Test%肝素抗凝血浆用于急诊生化检验的可行性分析

    Institute of Scientific and Technical Information of China (English)



    目的:分析肝素抗凝血浆用于急诊生化检验的可行性。方法以医院130例受检者为研究对象,常规采集静脉血6 ml,均分分别注入普通干燥管(血清组)和肝素钠抗凝管(血浆组)中,观察两组患者生化检验结果。结果两组患者Na+、K+、GLU、GGT检验结果比较差异具有统计学意义(P<0.05);剩余生化检验指标结果比较差异无统计学意义(P>0.05)。结论肝素抗凝血浆具有分离血浆速度快、抗凝能力强的优点,在急诊生化检验中具有高的应用价值。%Objective To analyze heparin anticoagulant blood plasma used for biochemical test the feasibility of the emergency.Methods 130 cases of patients as the research objects,routine venous blood was colected 6 ml,half-and-half respectively devided into ordinary drying tube(serum) and heparin anticoagulant tube(plasma),biochemical test results were observed of two groups of patients.ResultsInspection results of Na+, K+,GLU,GGT in two groups of patients,the differences had statisticaly significant(P0.05).Conclusion The heparin anticoagulant blood plasma has fast separation of plasma,the advantages of the anticoagulant ability, in the emergency biochemical test has higher application value.

  20. 累积γ辐射对大鼠血液生化和血象指标的影响%Influence of Cumulative Gamma Irradiation on Hemogram and Biochemical Indicators in Rat Blood

    Institute of Scientific and Technical Information of China (English)

    郭月凤; 张慧芳; 张忠新; 邢利虹; 段志凯


    目的:探讨γ射线累积照射对大鼠血象和血液生化指标的影响。方法成年SD大鼠接受137 Cs γ射线照射(剂量率0.336 mGy/min),累积剂量分别为0.5、1.0和2.0 Gy。照射结束后第2天和第11天,检测大鼠血象、WBC分类和血液生化指标。结果γ射线累积照射可造成受照大鼠WBC数在照后第2天显著降低;当累积剂量为1.0 Gy和2.0 Gy时,淋巴细胞比例持续降低,单核细胞比例持续升高,粒细胞比例暂时升高和HGB水平延迟性降低;血液生化指标中除尿酸外其它未见明显变化。结论γ射线累积照射对血液WBC及其分类有明显影响,对大鼠血液生化指标无明显影响。%This paper is to investigate the effects of cumulative gamma irradiation on hemogram and biochemi-cal indicators in rats blood .Adult SD rats were exposed to 137 Cs gamma ray at 0 .336 mGy/min ,and cumula-tive dose was 0 .05 ,0 .20 ,0 .5 ,1 .0 and 2 .0 Gy ,respectively .Hemogram ,WBC classification and biochem-ical indicators were detected at the second d and 11th d after exposure in rats blood .Gamma irradiation caused the number of WBC to decrease significantly at two days after exposure .Compared with control rats ,lympho-cyte ratio in exposed rats was consecutively lower ,monocyte ratio was consecutively higher ,granulocyte ratio and hemoglobin level delayed reduced ,when cumulative dose is 1 .0 Gy and 2 .0 Gy .There was no obvious change in blood biochemical indicators except acidum uricum .Cumulative gamma irradiation influences signifi-cantly WBC number and its classification in rats blood .

  1. Perubahan Nilai Hematologi, Biokimia Darah, Bobot Organ dan Bobot Badan Tikus Putih pada Umur Berbeda (THE CHANGES ON HEMATOLOGICAL, BLOOD BIOCHEMICAL VALUES, ORGAN AND BODY WEIGHT OF RAT AT DIFFERENT AGES

    Directory of Open Access Journals (Sweden)

    Marice Sihombing


    Full Text Available Research and development of health science require an animal model which has been known for itsorigin and characteristics. One of experimental animal model which is commonly used is albino rat. Thepurpose of this study was to investigate weight of organs (kidney, liver, spleen, and lung, hematologicalvalues (hemoglobin, hematocrit, erythrocyte and leucocyte, and the values of the biochemical blood (SGPT,SGOT, glucose and total protein of the albino rat at different ages. This study used 60 rats, which weredivided into 3 groups based on age namely 1, 2, and 3 months and groups based on sex that each groupconsisted of 10 males and 10 females. Samples of age and sex groups of rat were taken randomly. Eachcage consisted of 5 rats with the same ages and sex. Those rats fed and tap water ad libitum. The rats weresacrificed anaesthetically by with ether to take their blood and measure their organ‘s weight. Data wasanalyzed using one way ANOVA test, except data from heart organ which was analyzed using nonparametrictest (Friedman Test. To find out the increase of rats body weight age 1 -3 months, it was usedregression linier test. Results of statistic showed that there were significantly difference (p < 0,05 in bodyweight change, average of hematological values, blood biochemical values and organs weight in accordancewith increasing of rats age in all age groups. In contrast, average of hematocrit values had no significantlydifference (p > 0,05. Generally, male rats were bigger than female rats but there was no difference in all

  2. [Effect of stress actions on some hematologic and biochemical parameters of rat blood and on energetic intermolecular interactions in lipid extract under effect of light radiation]. (United States)

    Zabelinskiĭ, S A; Chebotareva, M A; Tavrovskaia, T V; Skverchinskaia, E A; Shukoliukova, E P; Maslov, M N; Krivchenko, A I


    Comparative study has been carried of effect of the three-day long starvation, running, and their combination on morphological parameters of rat blood, lipid metabolism, and activity of blood Na,K-ATPase. Different effect has been shown of these stress factors on the blood erythrocyte composition. Starvation is accompanied by the most pronounced release of stored erythrocyte into blood, which results in a significant decrease both of the total amount of reticulocytes and the complete absence of reticulocytes of the I stage of maturity (the youngest). The running on treadmill led to a significant increase of the total amount of blood reticulocytes and to multiple increase of immature reticulocytes (RC-I and RC-II), which can indicate some stress of the bone marrow erythroid stem line. The curve of acid resistance of blood reticulocytes has shown the animal to experience the greatest stress at a combination of starvation and running. Starvation and running produced different effects on blood lipid characteristics. The content of triacylglycerides (TAG) in blood rose by 40% at starvation and decreased by 30% at running, a similar tendency being found for index of atherogeneity. The fatty acid composition of blood phospholipids at running and its combination with starvation practically did not differ from control. A change of Na,K-ATPase, which is so characteristic of reaction to various kinds of stress, sharply fell at starvation (by 22%), but increased at running (by 13%) and decreased markedly at combination of these actions. Absorption spectra of lipid extracts of the whole blood of the rats submitted to various stress actions showed that extracted from blood (at different amount depending on the kind of action) is an organic substance with coupled bonds, which absorbs light in the diapason of 360-620 nm. The absorption of light in the diapason of 400-410 nm has been found to belong to the Soret band of ferroheme and ferriheme. The shift of the Soret band indicates

  3. Effective evaluation on application of blood high efficient coagulant in biochemical tests%血液高效促凝剂应用于生化检验的效果评价

    Institute of Scientific and Technical Information of China (English)



    Objective To evaluate the coagulant effect of a high efficient coagulant and investigate the effect on biochemical test results .M ethods The 25 items of 20 random samples were detected with an automatic biochemical analyzer .The results between the samples with and without the blood efficient coagulant were compared .Results The samples with the high blood coagulant showed a clear boundary between blood and serum ,clear serum and no tube wall phenomenon after centrifugal separation .The resulted serums were very ideal.However,there was no statistical difference in all results of biochemical tests between the samples with and without the coagulant.Conclusion The use of the high efficient coagulant can obtain ideal serum specimens and has no effect on results of bio -chemical tests.Therefore,it has a high application value .%目的:评价血液高效促凝剂的促凝效果及其对生化检验项目结果的影响。方法使用全自动生化分析仪平行检测20个随机样本的25项生化指标,比较添加与未添加血液高效促凝剂实验生化结果。结果添加高效促凝剂的样本血清与血细胞分界清晰、血清清澈、无管壁现象,所得血清非常理想,25项生化指标检测结果与未添加高效促凝剂差异无统计学意义(P >0.05)。结论血液高效促凝剂能够有效促进血液凝集,得到理想血清,并对生化检验结果无影响,具有很高的使用价值。

  4. [Biochemical parameters of blood and morpho-functional state of the liver of experimental animals by the actions of lead sulfide nanoparticles in different time study]. (United States)

    Omel'chuk, S T; Aleksiĭchuk, V D; Sokurenko, L M


    Biochemical studies revealed that alanine aminotransferase levels changing first during short action (30 injections) of lead sulfide nanoparticles of size 10 and 30 nm, and the ionic form of a 400 nm lead while the growth of both enzymes (aspartate aminotransferase and alanine aminotransferase) activity during long-term exposure (60 injections) is the same intensity. It it confirmed by the value of de Ritis coefficient, which is statistically the same as control. Morphological studies also confirm these data--degenerative changes of hepatocytes, reactive changes of the stroma and vascular responses were detected. It is shown that the severity of metabolic and morphological damages in the liver increased with prolonging the duration of lead nanoparticles intake.

  5. Artificial blood

    Directory of Open Access Journals (Sweden)

    Sarkar Suman


    Full Text Available Artificial blood is a product made to act as a substitute for red blood cells. While true blood serves many different functions, artificial blood is designed for the sole purpose of transporting oxygen and carbon dioxide throughout the body. Depending on the type of artificial blood, it can be produced in different ways using synthetic production, chemical isolation, or recombinant biochemical technology. Development of the first blood substitutes dates back to the early 1600s, and the search for the ideal blood substitute continues. Various manufacturers have products in clinical trials; however, no truly safe and effective artificial blood product is currently marketed. It is anticipated that when an artificial blood product is available, it will have annual sales of over $7.6 billion in the United States alone.

  6. Artificial blood. (United States)

    Sarkar, Suman


    Artificial blood is a product made to act as a substitute for red blood cells. While true blood serves many different functions, artificial blood is designed for the sole purpose of transporting oxygen and carbon dioxide throughout the body. Depending on the type of artificial blood, it can be produced in different ways using synthetic production, chemical isolation, or recombinant biochemical technology. Development of the first blood substitutes dates back to the early 1600s, and the search for the ideal blood substitute continues. Various manufacturers have products in clinical trials; however, no truly safe and effective artificial blood product is currently marketed. It is anticipated that when an artificial blood product is available, it will have annual sales of over $7.6 billion in the United States alone.

  7. Baseline blood pressure, low- and high-density lipoproteins, and triglycerides and the risk of vascular events in the Stroke Prevention by Aggressive Reduction in Cholesterol Levels (SPARCL) trial

    DEFF Research Database (Denmark)

    Amarenco, Pierre; Goldstein, Larry B; Callahan, Alfred


    AND RESULTS: The SPARCL trial randomized 4731 patients with recent stroke or transient ischemic attack (TIA) and no known coronary heart disease and LDL-C between 100 and 190 mg/dL to either atorvastatin 80 mg/d or placebo. Baseline assessment included SBP, DBP and measurements of low-density lipoprotein....... There were no interactions between any of these baseline variables and the effect of treatment on outcome strokes. CONCLUSIONS: In patients with recent stroke or TIA and no coronary heart disease, only lower baseline HDL-C predicted the risk of recurrent stroke with HDL-C, triglycerides, and LDL/HDL ratio...

  8. The Blood Biochemical Value Change of 20 Patients with Myocardial Infarction after Death%心肌梗死患者20例死后血液生化值变化的研究

    Institute of Scientific and Technical Information of China (English)

    孙进广; 林祥立; 郭雪美; 瞿勇强


    Objective Through inspecting the changes of four blood biochemical indexes (lactate dehydrogenase, muscle protein, creatine phosphokinase, creatine phosphokinase isozyme) in blood of 20 myocardial infarction deathes to obtain the data of biochemical value change, to explore the Forensic significance of change rule after death in diagnosis myocardial infarction and identification of the cause of death.Methods We took subclavian vein blood 10 mL from 20 diagnosed myocardial infarction in different time after the death.The 20 cases were taked from the judicial authentication center of Kunming Medical University.The blood samples were centrifuged for 5 minutes at 3, 000 r/min to seperste blood visible part.The plasma was tested by automatic blood biochemical analyzer.The organs and tissues in Autopsy were fixed with 10% formalin liquid,and stained by under hematoxylin-eosin stain (HE) dyeing to observe pathological morphology change under microscope.Results With the death time extend, the activities of Lactate dehydrogenase, muscle calcium protein, creatine phosphokinase, creatine phosphokinase isozyme increased gradually, they were significant different from traffic accident death group.Conclusion The increased activities of Lactate dehydrogenase,muscle calcium protein, creatine phosphokinase, creatine phosphokinase isozyme is helpful for myocardial infarction diagnosis and the cause of death identification.%目的 通过检测20例心肌梗死患者死后血液中乳酸脱氢酶、肌钙蛋白、肌酸磷酸激酶、肌酸磷酸激酶同工酶四个血液生化指标值的变化,获得生化值改变的数据资料,探索其死后变化规律和用于诊断生前心肌梗死及鉴定死亡原因的法医学意义.方法 采取昆明医学院司法鉴定中2009年至2010年间20例确诊心肌梗死死者死后不同时间锁骨下静脉血10 mL,血样低温高速离心机3 000 r/min 5min分离血液有形成分,其血浆经全自动血液生化仪检

  9. Analysis of the Value of Rapid Blood Glucose Meter and Routine Biochemical Analyzer in Clinical Blood Glucose Test%临床血糖检验中快速血糖仪与常规生化仪应用的价值分析

    Institute of Scientific and Technical Information of China (English)



    目的:探究临床血糖检验中快速血糖仪与常规生化仪的临床应用效果。方法选取2013年4月~2014年4月于我院就诊并且接受血糖检验的68例患者,采用回顾性分析法对所有患者的临床资料进行分析,并同时给予68例患者快速血糖仪与常规生化仪检验,对比两种检验方法的检验效果。结果快速血糖仪的血糖测量值为(5.81±0.02) mmol/L,常规生化仪的血糖测量值为(5.64±0.03)mmol/L,差异不具有统计学意义(P>0.05)。结论快速血糖仪具有高效、快捷、经济等特点,有利于诊断和治疗。%Objective Explore the clinical effect of rapid clinical blood test blood glucose meter and conventional biochemical analyzer.Methods 68 cases of patients receiving blood sugar test were chosen from April 2013 to April 2014 in our hospital, the use of a retrospective analysis of clinical data for all patients were analyzed, and 68 patients while giving fast meter with conventional biochemical analyzer test, test the effect of comparison of the two test methods.Results The test result of blood glucose is (5.81±0.02) mmol/L when using rapid blood glucose meter and the test result of blood glucose is (5.64±0.03) mmol/L when using conventional biochemical analyzer. The difference of results between two methods is not statistically significant (P>0.05).Conclusion The rapid blood glucose meter is an efifcient, fast, economic test method. It helps to diagnosis and treatment.

  10. Protective Effects of Vitamin C (Ascorbic Acid in Lead Acetate Exposed Diabetic Male Rats: Evaluation of Blood Biochemical Parameters and Testicular Histopathology

    Directory of Open Access Journals (Sweden)

    Alireza AYOUBI


    Full Text Available The aim of this study was to investigate the protective effects of vitamin C against lead toxicity by measuring the blood parameters and studying histopathology of testis in diabetic male rats. Wister rats (42 were randomly assigned into7 groups: I healthy; II fed lead acetate only; III vitamin C administered only; IV diabetic; V diabetic rats administered by vitamin C; VI diabetic rats given lead acetate and VII diabetic rats received lead acetate and vitamin C. The diabetic and lead groups had higher glucose, cholesterol, LDL, triglycerides and lower insulin and HDL concentration than the control group. It was found that vitamin C administration led to a lower level of blood glucose, cholesterol, LDL and triglycerides and higher HDL concentration in diabetic rats significantly. It was concluded that the antioxidant property of vitamin C resulted in reducing the oxidative stress complications of toxic levels of lead acetate in diabetic rats.

  11. Effect of the incorporation of date pits and orange pulp in rations composed of wheat straw and concentrate on the blood biochemical parameters of Ouled Djellal breeding

    Directory of Open Access Journals (Sweden)

    N. Lakhdara


    Full Text Available Twenty four lambs of Ouled Djellal breeding from the region of Constantine, Algeria, were assigned randomly into 4 groups, the mean initial weights within the groups, ranged between 37.6±4.27 and 39.8±5.41 kg, to investigate the effect of the incorporation of two by-products of food industry, fresh orange pulp, ground date pits in rations composed of wheat straw as roughage, and concentrate as supplement. Four feeding groups were formed, the first group (T1 was fed with wheat straw and concentrate (60%/40%, the second group (T2 with wheat straw and orange pulp (60%/40%, for group 3 and 4 (T3-T4, the diet consisted on a mixture of 60% wheat straw and date pits at a ratio of (80 to 20% as a roughage in addition to 40% orange pulp for T3 and 40% concentrate for T4. Blood samples were collected from the jugular vein before morning feeding. Values of animal's plasma levels of Ca, glucose, proteins and urea were measured using a UV spectrophotometer. There was no significant difference in all the diets for Ca value, Ca values varied between 8.37 and 10.74 mg/Dl. T4 showed the highest value. Glucose blood content was similar for all the animals with no significant differences. While a very significant difference <0.001 was observed in blood proteins level in T3 and T4 comparing to the other groups. When date pits were incorporated in the diet containing wheat straw and concentrate, a very significant difference on urea blood content of lambs was observed (P<0.001.

  12. Self-reported alcohol intake is a better estimate of 5-year change in blood pressure than biochemical markers in low resource settings: the PURE study


    Zatu, Mandlenkosi C.; Van Rooyen, Johannes M.; Du Toit, Loots; Wentzel-Viljoen, Edelweiss; Greeff, Minrie; Schutte, Aletta E.


    Background: Despite criticism of self-reported alcohol intake, it is a valuable tool to screen for alcohol abuse as a risk factor for cardiovascular disease. We aimed to compare various self-reported estimates of alcohol use with γ-glutamyltransferase (GGT) and percentage carbohydrate deficient transferrin (%CDT) considering their relationship with blood pressure changes (%BP) over a 5-year period in black South Africans. Method: We recruited 1994 participants and collected 5-year followe...

  13. 波纹唇鱼血细胞显微结构和血液生化指标%Microstructure of Peripheral Blood Cell and Serum Biochemical Indices of Cheilinus undulatus

    Institute of Scientific and Technical Information of China (English)

    廖光勇; 区又君; 李加儿


    以Wright's和Giemsa双重染色法对波纹唇鱼(Cheilinus undulatus)外周血细胞的形态进行显微观察研究并计数,同时测定各类血细胞的大小及血液的生化指标.血涂片观察发现:波纹唇鱼的外周血细胞可区分出红细胞、嗜中性粒细胞、单核细胞、淋巴细胞、血栓细胞等五种血细胞,未观察到嗜碱性粒细胞、嗜酸性粒细胞.在外周血液中还观察到核影、正在分裂的红细胞、幼稚红细胞和降解的红细胞.各类血细胞胞体由大到小的次序依次为单核细胞、嗜中性粒细胞、红细胞、大淋巴细胞、血栓细胞、小淋巴细胞.血浆中红细胞为(3.15±0.79)×1012/L,白细胞为(1.42±0.71)×1010/L,红细胞数远远多于白细胞.血液生化指标与其他鱼类相比有一定差异,表明鱼类血液的生化成分因种和生活环境而异.%The microscopic observation and count are conducted for the peripheral blood cells of C. Undula-tus by means of the Wright's and Giemsa double dye method. The serum biochemical indices of C. Undula-tus , the sizes of the various blood cells are determined. From the stained smears, five blood cell types I. E. Erythrocyte, neutrophil, monocyte, lymphocyte and thrombocyte are detected. Neither eosinophilic granu-locyten nor basophilic granulocyte is found but, some nuclear shadow of erythrocytes, immature erythro-cytes, direct dividing or dissolving erythrocytes. Among these cells, the sizes are put into an order from large to small as follows monocyte, neutrophil, erythrocyte, large lymphocyte, thrombocyte and small lymphlcyte. The erythrocyte and leucocyte concentrations in the blood are (3. 15 ±0. 79) × 1012/L and (1. 42 + 0. 71) × 1010/L respectively. The number of erythrocyte is much more than the leucocyte in the blood. The serum biochemical indices are different from other fish species. Therefore it is indicated that the serum biochemical indices for various fishes depend upon their species and

  14. Magic Baseline Beta Beam

    CERN Document Server

    Agarwalla, Sanjib Kumar; Raychaudhuri, Amitava


    We study the physics reach of an experiment where neutrinos produced in a beta-beam facility at CERN are observed in a large magnetized iron calorimeter (ICAL) at the India-based Neutrino Observatory (INO). The CERN-INO distance is close to the so-called "magic" baseline which helps evade some of the parameter degeneracies and allows for a better measurement of the neutrino mass hierarchy and $\\theta_{13}$.

  15. The TDAQ Baseline Architecture

    CERN Multimedia

    Wickens, F J

    The Trigger-DAQ community is currently busy preparing material for the DAQ, HLT and DCS TDR. Over the last few weeks a very important step has been a series of meetings to complete agreement on the baseline architecture. An overview of the architecture indicating some of the main parameters is shown in figure 1. As reported at the ATLAS Plenary during the February ATLAS week, the main area where the baseline had not yet been agreed was around the Read-Out System (ROS) and details in the DataFlow. The agreed architecture has: Read-Out Links (ROLs) from the RODs using S-Link; Read-Out Buffers (ROB) sited near the RODs, mounted in a chassis - today assumed to be a PC, using PCI bus at least for configuration, control and monitoring. The baseline assumes data aggregation, in the ROB and/or at the output (which could either be over a bus or in the network). Optimization of the data aggregation will be made in the coming months, but the current model has each ROB card receiving input from 4 ROLs, and 3 such c...

  16. The Potential Biochemical Diagnosis Criteria and Therapeutic Effect Indexes: Sex Hormone Binding Globulin Levels and Free Androgen Index in Blood of Patients With Polycystic Ovary Syndrome (United States)


    The Investigators Collected 534 PCOS Patients as the Case Group,and 580 Infertile Women With Normal Ovulatory Cycle of the Control Group;; At the Same Time, the Investigators Continuedly Collect Cases to October 2012, and Totally Collected 579 Patients With PCOS Altogether;; 534 Patients in the Cases Group and 580 Women in the Control Group Received no Measures, While 579 Patients Received Drugs;; The Investigators Monitored Basic Indexes in Blood of All the Subjects in This Suvey,and Also Monitored Indexes of 579 Patients After Treatment.

  17. Stability of 35 biochemical and immunological routine tests after 10 hours storage and transport of human whole blood at 21°C

    DEFF Research Database (Denmark)

    Henriksen, Linda O; Faber, Nina R; Moller, Mette F;


    thyroxine, immunoglobulin A, immunoglobulin M, orosomucoid, sodium, transferrin, and triglycerides met goals for imprecision, though they showed a minor, but statistically significant bias in results after storage. Cobalamin, folate, HDL-cholesterol, iron, phosphate, potassium, thyroid stimulating hormone...... and urate warranted concern, but only folate and phosphate showed deviations of clinical importance. CONCLUSIONS: We conclude that whole blood in lithium-heparin or serum tubes stored for 10 h at 21 ± 1°C, may be used for routine analysis without restrictions for all investigated analytes but folate...... and phosphate....

  18. 明胶微球阻塞胎羊胎盘后血气及生化指标的变化%Alteration of Fetal Blood-Gas, pH and Biochemical Parameters after Placental Occlusion in Ewe

    Institute of Scientific and Technical Information of China (English)



    目的 建立胎儿窘迫的妊娠羊动物模型并观测胎羊窘迫的动态过程。方法 妊娠晚期绵羊(湖羊)16头,于妊娠116~125d进宫腔行胎儿手术,胎羊股动脉插管,使导管顶端达胎羊腹主动脉水平,留置导管于胎羊体内,经三通管接压力感受器,连续动态监测胎羊血压,定期采血。手术成功后经胎羊腹主动脉注射明胶微球颗粒,阻塞胎盘建立胎儿窘迫的动物模型。每次注射微球前后胎羊采血作血气分析,留置血清检测血乳酸、高密度脂蛋白(HDL)和血糖等。结果 16例中10例手术成功,经胎羊腹主动脉注射微球后,pH、p∞2、和Po2,与微球注射的次数(MS)均有明显的关系,相关系数分别为-0.724 0(P0.05)和0.141 2(P>0.05)。乳酸浓度与pH值之间有明显的相关性,pH值7.0以下时,乳酸浓度明显升高。结论 经胎羊腹主动脉注射微球的方法,可以定量地表达胎盘阻塞的程度,符合胎儿窘迫的临床表现;该模型可动态地观察胎儿窘迫的过程。%Purpose To establish an animal model of fetal distress and to evaluate the alteration of pH, blood gas and biochemical parameters at fetal artery blood in ewe. Methods Intrauterine surgeries were performed at 116 - 125 gestational days in 16 ewes for vascular catheterization at fetal abdominal aorta 0.5 ml of 1% microspheres suspension was injected per 15 to 20 minutes after the surgeries had been succeed. Fetal blood pressure was continually monitored, and the fetal blood were sampled through the catheter before and after injection of microsphere for measurement of pH,blood gas,lactic acid,and high density lipoprotein (HDL). Results The of 16 pregnant sheeps were successfully operated. The times of microsphere injection (MS) was significantly related to fetal blood pH ( - 0. 724 0,P<0.01), Po2 (0. 521 5 ,P<0.05), p∞2( - 0. 700 79, P < 0.01 ) and lactate acid ( r = 0.50, P < 0.05 ). There was

  19. Hematological and serum biochemical profile of apparently healthy hariana cattle heifers in northern India. (United States)

    Mahima; Singh, Krishna Veer; Verma, Amit Kumar; Kumar, Vinod; Singh, Shanker Kumar; Roy, Debashis


    The 'Hariana' breed of cattle has been proved to be highly adaptable to harsh climatic conditions and resistant to common diseases prevalent in India. In this study, the normal reference values of hematological and serum proteins and other blood biochemical parameters were determined in the heifers of Hariana breed maintained at Instructional livestock farm complex, DUVASU, Mathura, India. A total of twenty four animals were used in this study. Blood was taken aseptically from all the animals and transported to laboratory for hematological and biochemical analysis. The hematological parameters (Hemoglobin, total erythrocyte count, total leukocyte count, packed cell volume) and biochemical parameters (Total protein, total albumin, albumin globulin ratio, urea, creatinine, calcium, phosphorous, calcium phosphorous ratio, AST, ALT) values were statistically analyzed, mean and standard deviations were calculated and set as reference values. This study reported hematological and serum biochemical values which could serve as baseline information for comparison in conditions of nutrient deficiency, physiological and health status of Hariana cattle heifers in India.

  20. Haematological and biochemical alterations caused by epidural and intramuscular administration of xylazine hydrochloride in dromedary camels (Camelus dromedarius

    Directory of Open Access Journals (Sweden)

    Omid Azari


    Full Text Available This study was conducted in 16 healthy immature dromedary camels weighing 120-150 kg to evaluate and compare the effects of epidural and intramuscular injections of xylazine administered at 0.1 mg/kg and 0.2 mg/kg. Haematological parameters included haemoglobin, packed cell volume, total erythrocyte count and total leukocyte count. Biochemical parameters included alkaline phosphates, alanine aminotransferase, aspartate aminotransferase, blood urea nitrogen, creatinine and glucose. Parameters were measured at different intervals before (baseline and after the administration of drugs. Our study showed that the effect of xylazine on haematological and biochemical parameters is dose-dependant and is also related to the route of administration. The low dose of xylazine administered using both intramuscular and epidural methods showed minimal effects, whereas high doses of the drug, especially when injected intramuscularly, caused greater changes in haematological and biochemical parameters.

  1. Haematological and biochemical alterations caused by epidural and intramuscular administration of xylazine hydrochloride in dromedary camels (Camelus dromedarius). (United States)

    Azari, Omid; Molaei, Mohammad Mahdi; Emadi, Ladan; Sakhaee, Ehsanollah; Sharifi, Hamid; Mehdizadeh, Sara


    This study was conducted in 16 healthy immature dromedary camels weighing 120-150 kg to evaluate and compare the effects of epidural and intramuscular injections of xylazine administered at 0.1 mg/kg and 0.2 mg/kg. Haematological parameters included haemoglobin, packed cell volume, total erythrocyte count and total leukocyte count. Biochemical parameters included alkaline phosphates, alanine aminotransferase, aspartate aminotransferase, blood urea nitrogen, creatinine and glucose. Parameters were measured at different intervals before (baseline) and after the administration of drugs. Our study showed that the effect of xylazine on haematological and biochemical parameters is dose-dependant and is also related to the route of administration. The low dose of xylazine administered using both intramuscular and epidural methods showed minimal effects, whereas high doses of the drug, especially when injected intramuscularly, caused greater changes in haematological and biochemical parameters.

  2. The effects of different levels of Chlorella microalgae on blood biochemical parameters and trace mineral concentrations of laying hens reared under heat stress condition (United States)

    Moradi kor, Nasroallah; Akbari, Mohsen; Olfati, Ali


    This study was conducted to investigate the effect of different supplementation levels of Chlorella microalgae on serum metabolites and the plasma content of minerals in laying hens reared under heat stress condition (27.5-36.7 °C, variable). A total number of 378 (40 weeks of age, with mean body weight of 1390 ± 120 g) were randomly allocated to six treatments with seven replicates. The birds were randomly assigned to 6 treatments (C, T1, T2, T3, T4, and T5) with 7 replicate cages of 9 birds. C. microalgae at the rates of 100, 200, 300, 400, and 500 ppm with water were offered to groups T1, T2, T3, T4, and T5, respectively, while group C served as a control. At 71 days of trial, blood samples (14 samples per treatment) were taken for measuring serum metabolites and at 72 days for plasma mineral analysis. The results of this experiment showed that the supplementation of 200-500 ppm C. microalgae decreased the serum content of cholesterol, triglycerides, and LDL ( P HDL content increased ( P < 0.05) in the hens supplemented with C. microalgae (300 or 400 and 500 ppm). C. microalgae at rates of 300-500 ppm caused a marked ( P < 0.05) increase in the plasma content of manganese or iodine and selenium but other minerals were not statistically different among treatments. Overall, from the results of the present experiment, it can be concluded that supplementation of C. microalgae at high rates was beneficial on blood parameters of laying hens reared under heat stress.

  3. Effects of exposure to three environmental chemicals on the selected biochemical parameters of the blood plasma of rainbow trout, Oncorhynchus mykiss (Walbaum

    Directory of Open Access Journals (Sweden)

    Řehulka Jiří


    Full Text Available Rainbow trout Oncorhynchus mykiss at a weight of 115 ± 24 g (mean ± SD were experimentally injected with di(2-ethylhexylphthalate (DEHP, 2,3,7,8-tetrachlorodibenzo-p-dioxine (TCDD and gamma isomer of hexachlorocyclohexane (γ-HCH to evaluate nitrogen metabolism (total protein [TP], blood urea nitrogen, uric acid [UA], creatinine, carbohydrate metabolism (glucose, mineral metabolism (inorganic phosphate[P], total calcium [Cat], and catalytic activity of enzymes (alanine aminotransferase [ALT], aspartate aminotransferase [AST], alkaline phosphatase [ALP], lactate dehydrogenase [LD]. After 21 days, the tested xenobiotics, administered by the intraperitoneally route, caused the following effect: in comparison with negative controls, fish injected with DEHP at concentrations of 200 or 50 mg kg-1 of body weight were found to have a higher level of P and lower level of Cat and decreased catalytic concentrations of ALT, AST, ALP and LD in the plasma of the peripheral blood. A higher level of TP and P and a decreased catalytic concentration of ALT were found when TCDD was administered at a dose of 2 μg kg-1. A decrease in the catalytic concentrations of ALT and ALP occurred in fish injected with γ-HCH in a dose of 50 mg kg-1. UA and P levels were decreased in fish injected with γ-HCH in a dose of 5 mg kg-1. DEHP and TCDD caused neutrophilic leucocytosis with a marked left shift. Both concentrations of γ-HCH led to an increase in polychromatophilic erythrocytes. The frequency of micronuclei varied in all experimental groups, including the controls, ranging between 0.8 and 2.1 ‰. Histological examination in fish injected with DEHP at a concentration of 200 mg kg-1 revealed eosinophilic droplets in the epithelium of renal tubules and histological examination in fish injected with γ-HCH at a higher concentration revealed an increased activation of sinusoidal cells in the liver, a fibrous thickening of bile duct walls, bile duct hyperplasia and a


    Directory of Open Access Journals (Sweden)

    Nilma Cintra Lea


    Full Text Available Background: In Brazil, listeriosis is not a notifiable disease; thus, the incidence of Brazilian cases remains unknown. Listeria monocytogenes is not always included in automated systems, and its detection depends on the high skill level of microbiology laboratory professionals. This paper describes the characteristics of L. monocytogenes isolates fortuitously obtained from an endocarditis case in Recife, PE, Brazil. Methods: Six bacterial isolates obtained from six blood cultures from a 28-year-old male bearing a prosthetic mitral heart valve were analyzed by PCR using primers specific of L. monocytogenes to confirm a presumptive identification, determine the serotype and presence of the virulence genes (inlA, inlB, inlC, inlJ, hly, plcA, actA, prfA in an attempt to determine the Listeria genotype by PCR-ribotyping. Results: The samples were identified as L. monocytogenes 4b. All investigated virulence genes were amplified by PCR, and the identity of the amplified segments was confirmed by sequencing. A deletion of 105 base pairs was detected in the actA gene. All of the samples generated the same PCR-ribotype pattern, clustered into a single ribotype, and were considered a single strain. Conclusion: L. monocytogenes infection should be considered in endocarditis differential diagnoses, especially among high-risk groups, due to its high pathogenicity and the environmental ubiquity.


    Directory of Open Access Journals (Sweden)

    V. N. Kim


    Full Text Available The function of endothelium of microcirculation vessels, vegetative and psychoemotional status, lipid spectrum and physical efficiency in 43 young sportsmen are corrected with the aid of Tentorium api-phyto products (APP.The following tests were conducted: samples of reactive hyperemia and hyperventilation, ultrasound investigation of brachial artery, determination of performance efficiency, total cholesterin, triglycerides, cortisol, red cells, vegetative and psychoemotional status by the Spielberger’s test and SAN test, as well as biomicroscopy of sclera. To relief the psychophysical stress, improve the endothelium function and performance efficiency, the sportsmen received APP as a food additive.After the reception of APP, the lipid and cortisol levels decreased markedly, and the level of red cells increased. The aerobic capacity increased. The recovery time after exercise shortened. The vegetative stress level decreased significantly, and the psychoemotional status improved. The endothelium dysfunction removed.It is shown that APP is a new method of prophylaxis of sudden death in sport, in people with blood circulation pathology, creative professionals, and businessmen.

  6. Whole blood transcriptomics and urinary metabolomics to define adaptive biochemical pathways of high-intensity exercise in 50-60 year old masters athletes.

    Directory of Open Access Journals (Sweden)

    Kamalika Mukherjee

    Full Text Available Exercise is beneficial for a variety of age-related disorders. However, the molecular mechanisms mediating the beneficial adaptations to exercise in older adults are not well understood. The aim of the current study was to utilize a dual approach to characterize the genetic and metabolic adaptive pathways altered by exercise in veteran athletes and age-matched untrained individuals. Two groups of 50-60 year old males: competitive cyclists (athletes, n = 9; VO2peak 59.1±5.2 ml·kg(-1·min(-1; peak aerobic power 383±39 W and untrained, minimally active individuals (controls, n = 8; VO2peak 35.9±9.7 ml·kg(-1·min(-1; peak aerobic power 230±57 W were examined. All participants completed an acute bout of submaximal endurance exercise, and blood and urine samples pre- and post-exercise were analyzed for gene expression and metabolic changes utilizing genome-wide DNA microarray analysis and NMR spectroscopy-based metabolomics, respectively. Our results indicate distinct differences in gene and metabolite expression involving energy metabolism, lipids, insulin signaling and cardiovascular function between the two groups. These findings may lead to new insights into beneficial signaling pathways of healthy aging and help identify surrogate markers for monitoring exercise and training load.

  7. Effects of buffering mineral mixtures on milk yield, milk composition, rumen pH and some blood biochemical parameters in heat stressed dairy cows

    Directory of Open Access Journals (Sweden)

    Adamović Milan


    Full Text Available The objective of the work was to investigate the influence of partial substitution of magnesium oxide with natural bentonite in feed mixtures used in feeding of cows during their exposure to heat stress. The investigation lasted 30 days and was carried out during last ten days of may and first twenty days of June when average air temperature in stables was 36.6±2.5oC. In the experiment there were included 30 cows of Holstein breed in first phase of lactation, which were divided into two groups of 15 cows: control (C and experimental (E. Group C was fed with experimental mineral mixture that contained 60% of magnesium oxide during the whole investigation period. Group E was fed with experimental mineral mixture that contained 40% of magnesium oxide as well as 20% of natural bentonite. Remaining ingredients in both control and experimental mineral mixtures were the same and also contained 20% of sodium bicarbonate and 20% of zeolite in the same quantities. The control and experimental mineral mixtures were mixed into complete feed mixture (18% UP in the amount of 1%. At tne end of the investigation period, on the 30th day, there were taken samples of rumens contents for determining pH, and after that blood samples, in which, after the separation of blood serum, were determined glucose concentration, total proteins, albumin, globulin, urea, HDL cholesterol, LDL cholesterol, total bilirubin, calcium and phosphorus, as well as the activity of ALT and AST. By computation there was calculated the ratio between albumin and globulin, ALT and AST, and the ratio between calcium and phosphorus. Daily allowance and milk chemical composition ( percentage of fat, proteins and dry substance were determined at the end of the investigation period, that is on the 30th day of lactation, for each cow individually. Partial substitution of magnesium oxide with bentonite influenced milk production increase, but it was statistically insignificant. Besides that, in E

  8. 苯致白血病患者外周血细胞和生化指标的分析%Analysis of peripheral blood cells and biochemical parameters of benzene-induced leukemia

    Institute of Scientific and Technical Information of China (English)

    杨祥丽; 王佃鹏; 周飞; 张艳芳; 李培茂; 张志敏; 邓丽丹; 罗斌


    目的:分析职业性苯致慢性粒细胞白血病外周血象及肝功能、肾功能、血糖、血脂参数变化规律。方法选取61例健康体检者作为对照组,10例苯致慢性粒细胞白血病患者作为病例组,对两组研究对象进行外周血细胞计数分析和血清生化(肝功能、肾功能、葡萄糖、血脂)参数检测分析。结果病例组的红细胞、血红蛋白、红细胞压积、红细胞分布宽度、平均红细胞体积、白细胞、中性粒细胞绝对值、淋巴细胞绝对值、嗜碱性细胞绝对值、血小板比积、大血小板比率、总蛋白、清蛋白、球蛋白、总胆红素、直接胆红素、间接胆红素、葡萄糖、总胆固醇、高密度脂蛋白胆固醇、低密度脂蛋白胆固醇、尿酸和对照组比较,差异有统计学意义(P<0.05)。结论苯致慢性粒细胞白血病患者血细胞参数异常,肝肾功能、血脂及存在异常,定期监测这些指标具有重要意义。%Objective To investigate the liver function ,kidney function ,glucose ,lipids and blood cell sparameter changes of ben‐zene‐induced chronic myeloid leukemia cases in peripheral blood .Methods 61 normal healthy volunteers as the control groups ,10 benzene‐induced chronic myeloid leukemia patients as the case groups .Peripheral blood cell were counted and serum biochemical pa‐rameter(liver function ,kidney function ,glucose ,blood lipids) wer detected .Results The parameters including of RBC ,hemoglobin , hematocrit ,red blood cell distribution width ,mean corpuscular volume ,leukocytes ,absolute neutrophil count ,absolute lymphocyte , basophils ,platelet hematocrit ,large platelet ratio ,total protein ,albumin ,globulin ,total bilirubin ,direct bilirubin ,indirect bilirubin , alkaline phosphatase ,total cholesterol ,high density lipoprotein cholesterol ,low density lipoprotein cholesterol ,creatinine ,uric acid , creatine kinase ,lactate dehydrogenase ,

  9. 赛前训练对击剑运动员生化、免疫及贫血指标的影响%Influence of Pre-Event Training on Blood Biochemical, Immune and Anemic Indexes of Fencers

    Institute of Scientific and Technical Information of China (English)

    殷正云; 姜辉; 田亚平; 向荣


    通过研究赛前训练对击剑运动员血液中生化、免疫以及贫血指标的影响,为教练科学合理地安排训练强度,避免疲劳提供依据.分别采集22名击剑运动员在训练前、训练后2h和训练后24h的空腹静脉血,测定其内生化、免疫和贫血等15项研究指标.结果表明,监测的生化指标在训练后均呈先升高后降低的趋势.CK的水平变化最为显著,训练后2h的水平是训练前的2倍多.训练后2h,CD3+CD56+和CD3-CD19+显著升高(P<0.01),CD3+CD4+和CD3+CD8+略下降但CD4+/ CD8+值不变.训练后24h,各监测免疫指标与训练前比较均升高.贫血指标Hb ﹑RBC﹑HCT在三个阶段均呈逐渐下降的趋势,而且女运动员的下降趋势比男运动员更加显著.结果提示,赛前训练强度和休整时间比较合理,训练对运动员肝脏、心脏和肾脏没有实质性损伤且使运动员的机体免疫能力得到了提高.训练后,女运动员比男运动员更容易出现运动性疲劳﹑贫血等情况,而且恢复也较男运动员慢.%In order to furnish evidence for scientifically and reasonably arranging the intensity of training and preventing exhaustion, the influence of pre-event training on blood biochemical, immune and anemia indexes of fencers was studied. Fasting venous blood of 22 fencing athletes was collected at three time points: before training, 2 hours after training, and 24 hours after training. In total, 15 indexes including biochemical, immune and anemia indexes were detected. The results show that all the detected biochemical indicators after training were increased firstly and then decreased, especially the change of ck, its level was doubled 2 hours after training. The percentages of CD3 +CD56 +and CD3 + CD19 + were significantly increased ( P <0.01 ). Although the percentage of CD3 + CD4 + and CD3 + CD8 + were decreased, the ratio of CD4 + to CD8 + was unchanged. 24 hours after training, the level of all the detected immune indexes

  10. Comparison of blood glucose test results between point-of-care testing glucose meter and biochemical analyzer%POCT血糖仪与生化分析仪血糖检测结果的比对试验及分析

    Institute of Scientific and Technical Information of China (English)



    Objective To explore precision and accuracy of point-of-care testing(POCT) blood glucose meter through comparing blood glucose results between POCT glucose meter and biochemical analyzer. Methods Selected 4 sets of POCT glucose meter to detect the same sample and compared blood glucose results between Beckman CX-9 biochemical analyzer and POCT blood glucose meter. Results Measured results of Beckman CX-9 biochemical analyzer and POCT glucose meter were highly correlated(P>0.05), differences were not statistically significant. Conclusion POCT blood glucose meter is a consistent and convenient detection method for measuring blood glucose results as biochemical analyzer, so it is worthy of clinical promotion and application.%目的对比床旁检测(POCT)血糖仪与生化分析仪对血糖测定的结果,对POCT血糖仪的精密度与准确度进行探讨。方法选用4台POCT血糖仪对同一标本进行测定,并比较贝克曼CX-9生化分析仪与POCT血糖仪的测定结果。结果贝克曼CX-9生化分析仪与POCT血糖仪的测定结果高度相关(P>0.05),差异不具有统计学意义。结论 POCT血糖仪与生化分析仪对血糖的测定结果具有一致性,且方便快捷,可作为急诊的检测方法,值得在临床过程中推广、应用。

  11. Effect of f Noise Stress on Blood Biochemical Parameters of Laying Hens%噪声应激对蛋鸡血液生化指标的影响

    Institute of Scientific and Technical Information of China (English)

    苏燕芳; 齐大胜; 韦彩利; 程丽丽; 杨松; 陈辉; 黄仁录


    The objective of this research was to study the effect of noise stress on blood biochemical parameters of hens. At the age of 500 day, 120 Hyline (Brown) commercial hens were randomly assigned to 3groups, with four rep- lications per group, 10 chickens in%500日龄海兰褐商品蛋鸡120只,随机分为3组,每组4个重复,每个重复10只鸡。研究噪声应激对蛋鸡血液生化指标的影响。结果表明,噪声对甘油三酯、葡萄糖、钙、磷及总胆固醇含量及肌酸激酶、乳酸脱氢酶、碱性磷酸酶、丙氨酸氨基转移酶活性影响差异不显著(P〉0.05)。日粮中添加60IU/kg的维生素E对缓解应激起到一定作用(P〉0.05)。

  12. Long Baseline Neutrino Experiments (United States)

    Mezzetto, Mauro


    Following the discovery of neutrino oscillations by the Super-Kamiokande collaboration, recently awarded with the Nobel Prize, two generations of long baseline experiments had been setup to further study neutrino oscillations. The first generation experiments, K2K in Japan, Minos in the States and Opera in Europe, focused in confirming the Super-Kamiokande result, improving the precision with which oscillation parameters had been measured and demonstrating the ντ appearance process. Second generation experiments, T2K in Japan and very recently NOνA in the States, went further, being optimized to look for genuine three neutrino phenomena like non-zero values of θ13 and first glimpses to leptonic CP violation (LCPV) and neutrino mass ordering (NMO). The discovery of leptonic CP violation will require third generation setups, at the moment two strong proposals are ongoing, Dune in the States and Hyper-Kamiokande in Japan. This review will focus a little more in these future initiatives.

  13. Biofuels Baseline 2008

    Energy Technology Data Exchange (ETDEWEB)

    Hamelinck, C.; Koper, M.; Berndes, G.; Englund, O.; Diaz-Chavez, R.; Kunen, E.; Walden, D.


    The European Union is promoting the use of biofuels and other renewable energy in transport. In April 2009, the Renewable Energy Directive (2009/28/EC) was adopted that set a 10% target for renewable energy in transport in 2020. The directive sets several requirements to the sustainability of biofuels marketed in the frame of the Directive. The Commission is required to report to the European Parliament on a regular basis on a range of sustainability impacts resulting from the use of biofuels in the EU. This report serves as a baseline of information for regular monitoring on the impacts of the Directive. Chapter 2 discusses the EU biofuels market, the production and consumption of biofuels and international trade. It is derived where the feedstock for EU consumed biofuels originally come from. Chapter 3 discusses the biofuel policy framework in the EU and major third countries of supply. It looks at various policy aspects that are relevant to comply with the EU sustainability requirements. Chapter 4 discusses the environmental and social sustainability aspects associated with EU biofuels and their feedstock. Chapter 5 discusses the macro-economic effects that indirectly result from increased EU biofuels consumption, on commodity prices and land use. Chapter 6 presents country factsheets for main third countries that supplied biofuels to the EU market in 2008.

  14. Hematology and blood biochemistry in wild hybrid marmosets from the Atlantic Forest, Brazil

    Directory of Open Access Journals (Sweden)

    Ita de Oliveira e Silva


    Full Text Available This paper aims to describe the hematological and biochemical values of wild hybrid marmoset (Callithrix penicillata and C. geoffroyi found in a forest zone of Southeastern Brazil. The marmosets were anaesthetized using ketamine and xylazine hydrochloride. Blood samples (0.5-1mL were collected through the venipuncture of the femoral vein. Hematological and biochemical analyses were performed using automated counters and biochemical kits. The comparison for sex (adult males vs. adult females and age class (juvenile vs. adult physiological data and weight were analyzed through Student's t-test for independent samples. Significant differences between sex were observed in erythrocytes (P<0.01 and hemoglobin (P<0.05. The present study provides essential baseline information on the normal blood values of wild hybrid marmosets, the data of which are not readily accessible from the existing body of scientific literature on nonhuman primates.

  15. 床旁血气分析仪与生化仪测定动静脉血钾浓度差异的检测%Detection of Potassium Difference Between Arterial and Venous Blood Measured by Bedside Blood Gas Analyzer and Automatic Biochemical Analyzer

    Institute of Scientific and Technical Information of China (English)

    江龙; 李鹏


    Objective To explore the difference between blood potassium measured by a bedside blood gas analyzer (Abbott i-STAT) and an automatic biochemical analyzer (ROCHE DDP). Methods In this study, arterial and venous blood samples are collected from 130 patients admitted to the intensive care unit (ICU). The concentrations of potassium and pHs in arterial samples are detected by Abbott i-STAT, while the concentrations of serum potassium in paired venous samples are detected by ROCHE DDP. All samples are divided into three groups. The difference between arterial and venous values are calculated according to different pH groups and made for analysis of variance. Results There is significant difference between results of blood potassium of Abbott i-STAT and ROCHE DDP (P<0.05). Blood potassium measured by the two devices is gradually rising when the pH is decreasing. There is no significant difference of blood potassium under different pH between the two groups. Conclusion The arterial potassium tested by bedside blood gas analyzer could not replace the venous potassium tested by biochemical analyzer, pH can affect the value of blood potassium, but the pH would not enlarge the difference of blood potassium measured by two methods above.%目的 分别采用床旁血气分析仪(Abbott i-STAT)和全自动生化仪(ROCHE DDP)测定重症患者动脉全血及静脉血清中的钾离子,分析在不同pH值下两种方法测定钾离子浓度的差异.方法同时采集130例ICU患者的动静脉血标本,采用Abbott i-STAT分析动脉全血的pH值和钾离子,ROCHE DDP测定静脉血清钾.按pH值分组计算动静脉血钾浓度差值,分析不同pH值情况下两种方法检测血钾的差异.结果 Abbott i-STAT测定动脉全血钾在各组均低于ROCHE DDP测定的静脉血清钾,差异有统计学意义(P <0.05).随着pH的降低,测得的动静脉钾离子浓度升高,但其浓度差在组间无统计学差异.结论床旁血气分析仪测得的动脉全血钾不

  16. Detection of IgM antibodies against Toxoplasma gondii in blood samples absorbed onto filter paper, in order to assess a baseline for the occurence of congenital toxoplasma infections

    NARCIS (Netherlands)

    Conyn-van Spaendonck MAE; van Knapen F


    This report deals in the first place with some investigations on the utility of blood samples absorbed onto filter paper for determination of antibodies to Toxoplasma gondii. Secondly, the results of specific IgM detection in such samples of a cohort of neonates born in 1986/1987 are reported. The

  17. Hematological and Biochemical Parameters in Elite Soccer Players During A Competitive Half Season (United States)

    Anđelković, Marija; Baralić, Ivana; Đorđević, Brižita; Stevuljević, Jelena Kotur; Radivojević, Nenad; Dikić, Nenad; Škodrić, Sanja Radojević; Stojković, Mirjana


    Summary Background The purpose of the present study was to report and discuss the hematological and biochemical behavior of elite soccer players, in order to get more insight in the physiological characteristics of these sportsmen and to provide trainers and sports doctors with useful indicators. Methods Nineteen male soccer players volunteered to participate in this study. We followed the young elite soccer players during a competitive half season. Venous blood samples were collected between 9:00 and 10:00 a.m. after an overnight fast (10 h) at baseline, after 45 and 90 days and hematological and biochemical parameters were measured. Results Hemoglobin and hematocrit levels were significantly reduced over the observational period (psoccer training (psoccer players are characterized by significant changes in biochemical and hematological parameters over the half season, which are linked to training workload, as well as adaptation induced by the soccer training. Although the values of the measured parameters fell within the reference range, regular monitoring of the biochemical and hematological parameters is fundamental for the identification of a healthy status and related optimal performances by sport doctors and trainers and selection of a correct workload by trainers.

  18. Effects of heme iron on growth performance and blood biochemical parameters of weaned piglets%血红素铁对断奶仔猪生长性能和血液指标的影响

    Institute of Scientific and Technical Information of China (English)

    高冬余; 王留香; 于伟; 江国永; 潘勇; 成国祥


    One hundred and twenty 28-day-old weaned piglets were selected and randomly divided into 4 groups, i.e. control, treatment 1, treatment 2 and treatment 3, which were respectively supplied (K27.5, 55.0 and 85.0 mg/kg heme iron on the basal diet. The effects of heme iron on growth performance and blood biochemical parameters (hemoglobin and IgG) of weaned piglets were researched. The feeding experiment was conducted for 13 days. The results as follows: heme iron could improve weaned piglet's average daily gain and feed conversion rate, Statistical analysis showed 55.0 mg/kg heme iron in diet increased feed conversion rate by 10.27% compared with control group (P<0.05). Heme iron had a certain growth promoting effect on weaned piglets, and the hemoglobin concentration in the whole blood and the IgG concentration in the plasma were improved.%选取120头28日龄断奶仔猪,随机分成4个处理组,即1个对照组和3个试验组,每组3个重复,每个重复10头.对照组饲喂基础日粮,试验1、2、3组分别饲喂基础日粮中血红素铁净添加量为27.5、55.0、85.0 mg/kg的3种试验日粮,研究日粮中添加血红素铁对断奶仔猪的生长性能、血液指标(血红蛋白浓度、免疫球蛋白浓度)的影响.试验正试期13d.结果表明,血红素铁各添加量组均能够提高仔猪的生长性能,日粮中血红素铁添加量为55 mg/kg时,仔猪平均日增重、饲料转化率较对照组分别提高12.02%和10.27%(P<0.05).血红素铁对断奶仔猪有良好的促生长作用,也在一定程度上提高了仔猪血液中血红蛋白和IgG浓度.

  19. Hanford Site technical baseline database

    Energy Technology Data Exchange (ETDEWEB)

    Porter, P.E., Westinghouse Hanford


    This document includes a cassette tape that contains the Hanford specific files that make up the Hanford Site Technical Baseline Database as of May 10, 1996. The cassette tape also includes the delta files that delineate the differences between this revision and revision 3 (April 10, 1996) of the Hanford Site Technical Baseline Database.

  20. Hanford Site technical baseline database

    Energy Technology Data Exchange (ETDEWEB)

    Porter, P.E.


    This document includes a cassette tape that contains the Hanford specific files that make up the Hanford Site Technical Baseline Database as of September 30, 1996. The cassette tape also includes the delta files that dellinate the differences between this revision and revision 4 (May 10, 1996) of the Hanford Site Technical Baseline Database.

  1. Plutonium Immobilization Project Baseline Formulation

    Energy Technology Data Exchange (ETDEWEB)

    Ebbinghaus, B.


    A key milestone for the Immobilization Project (AOP Milestone 3.2a) in Fiscal Year 1998 (FY98) is the definition of the baseline composition or formulation for the plutonium ceramic form. The baseline formulation for the plutonium ceramic product must be finalized before the repository- and plant-related process specifications can be determined. The baseline formulation that is currently specified is given in Table 1.1. In addition to the baseline formulation specification, this report provides specifications for two alternative formulations, related compositional specifications (e.g., precursor compositions and mixing recipes), and other preliminary form and process specifications that are linked to the baseline formulation. The preliminary specifications, when finalized, are not expected to vary tremendously from the preliminary values given.

  2. Comparative study on the blood physiological and biochemical indexes of snakehead with nine mating groups%九种交配组合鳢血液生理生化指标的比较分析

    Institute of Scientific and Technical Information of China (English)

    曾运丁; 王超; 周爱国; 邹记兴


    对来源于乌鳢(Channa argus)、斑鳢(C.maculate)及杂交鳢作为亲鱼彼此之间的交配繁殖得到的九种组合鳢的血液部分生理生化指标做了比较分析.其中G1-G9分别代表斑鳢(♀)×斑鳢(♂)、斑鳢(♀)×乌鳢(♂)、斑鳢(♀)×杂交鳢(♂)、乌鳢(♀)×斑鳢(♂)、乌鳢(♀)×乌鳢(♂)、乌鳢(♀)×杂交鳢(♂)、杂交鳢(♀)×斑鳢(♂)、杂交鳢(♀)×乌鳢(♂)、杂交鳢(♀)×杂交鳢(♂).结果显示:从血液生理指标来看,红细胞计数(RBC)结果为G2 >G4 >G1 >G5 >G8 >G9 >G7 >G6 >G3,白细胞计数(WBC)结果为G2 >G4 >G5>G6>Gl>G9 >G3 >G7 >G8,血栓细胞计数(PLT)结果为G2 >G1 >G4 >G5 >G8 >G9 >G7 >G6 >G3,血细胞压积(HCT)的结果为G2 >G1 >G4 >G5 >G8 >G9 >G7 >G3 >G6,血红蛋白(HGB)的结果为G2 >G1 >G4 >G5 >G8>G9> G6 >G7 >G3,平均红细胞蛋白含量(MCH)的结果为G4 >G5 >G2 >G1 >G6 >G8 >G9 >G7 >G3,平均红细胞蛋白浓度(MCHC)的结果为G4 >G5> G2 >G1 >G6 >G8 >G3 >G9 >G7,红细胞平均体积(MCV)和红细胞分布宽度(RDW-CV)无显著差异.从血液生化指标来看,总蛋白(TP)的大小为G5 >G4 >G2 >G1 >G6 >G3 >G7>G8>G9,谷草转氨酶(AST)的大小为G2 >G4 >Gl >G5 >G3 >G6 >G7 >G8 >G9,甘油三酯(TG)的大小为G5>G1 >G2 >G4 >G3 >G6 >G7 >G8 >G9,胆固醇(CHOL)和谷丙转氨酶(ALT)无显著差异.综合分析九种交配组合鳢的血液生理及生化指标可以看出,G2(斑鳢(♀)×乌鳢(♂))和G4(乌鳢(♀)×斑鳢(♂))相对于其他的组合有明显的“杂种优势”.%The blood physiological and biochemical indexes of nine groups snakehead which came from mating with each other of Channa argus,C.maculate and hybrid snakehead,were determined and comparative studied.G1-G9 represen-ted C.maculate (♀) × C.maculate (♂),C.maculate (♀) × C.argus (♂),C.maculate (♀) × hybrid

  3. Molt-associated changes in hematologic and plasma biochemical values and stress hormone levels in African penguins (Spheniscus demersus). (United States)

    Mazzaro, Lisa M; Meegan, Jenny; Sarran, Delphine; Romano, Tracy A; Bonato, Vinicius; Deng, Shibing; Dunn, J Lawrence


    Handling, including blood collection, has often been discouraged in molting penguins because it is considered an additional stress imposed on birds already experiencing major physiologic stress associated with molting. To evaluate the degree of physiologic stress posed by molting, we compared the hematologic and plasma biochemical values and hormone levels of molting and nonmolting African penguins, Spheniscus demersus. Five male and 5 female penguins randomly chosen were given complete physical examinations, were weighed, and blood samples were taken at 7 time points before, during, and after the molt. Data were analyzed by linear mixed-model analysis of variance. Throughout the study, behavior and appetite remained normal. Catecholamine levels were highly variable within and among subjects, whereas mean corticosterone levels were significantly different between baseline, molt, and postmolt values. Significant differences from baseline values were observed in many of the hematologic analytes; however, only decreases in hematocrit and red blood cell count values were considered clinically significant. Anemia due to experimentally induced blood loss as a possible cause of the significant hematologic changes was ruled out based on results of a follow-up control study during the nonmolt season, which showed no significant changes in hematocrit level or total red blood cell counts when using similar sampling protocols, which indicates that these changes were associated with molt.

  4. Oscillation Baselining and Analysis Tool

    Energy Technology Data Exchange (ETDEWEB)


    PNNL developed a new tool for oscillation analysis and baselining. This tool has been developed under a new DOE Grid Modernization Laboratory Consortium (GMLC) Project (GM0072 - “Suite of open-source applications and models for advanced synchrophasor analysis”) and it is based on the open platform for PMU analysis. The Oscillation Baselining and Analysis Tool (OBAT) performs the oscillation analysis and identifies modes of oscillations (frequency, damping, energy, and shape). The tool also does oscillation event baselining (fining correlation between oscillations characteristics and system operating conditions).

  5. Bioquímica plasmática de cascavéis (Caudisona durissa LINNAEUS, 1758 em cativeiro Blood biochemical parameters from captivity rattlesnake (Caudisona durissa LINNAEUS, 1758

    Directory of Open Access Journals (Sweden)

    Wellington Bandeira da Silva


    breeding stock, justifying the importance of studying these snakes' physiology. Blood biochemical analysis of 53 Caudisona durissa was performed using plasma samples in an automated equipment (Ciba Corning - Express Plus®. Mean values and its respective Standard deviations are: blood urea nitrogen (BUN -1.32mg dL-1 (±1.1; uric acid - 2.08mg dL-1 (±1.4; creatinine - 0.52mg dL-1 (±0.2; total protein - 3.7g dL-1 (±0.7; albumin - 1.62g dL-1 (±0.4; globulins - 2.08g dL-1 (±0.5; calcium - 15.25mg dL-1 (±2.8; phosphorus - 4.61mg dL-1 (±1.9; cholesterol - 171.58mg dL-1 (±52.7; triglycerides - 19.29mg dL-1 (±14.3; alkaline phosfatase - 31.04U L-1 (±12.4; aspartate aminotransferase (AST - 22.25U L-1 (±11.4; alanin aminotransferase (ALT 7.11U L-1 (±5.4; amylases - 1385.23U L-1 (±568.7. The following indexes were calculated: BUN/creatinine ratio - 2.5 and calcium/phosphorus ratio -3.3. Rattlesnake blood biochemical results above could be used as reference in clinical evaluation for captivity C. durissa.

  6. 324 Building Baseline Radiological Characterization

    Energy Technology Data Exchange (ETDEWEB)

    R.J. Reeder, J.C. Cooper


    This report documents the analysis of radiological data collected as part of the characterization study performed in 1998. The study was performed to create a baseline of the radiological conditions in the 324 Building.

  7. Quivira NWR biological baseline data (United States)

    US Fish and Wildlife Service, Department of the Interior — This dataset is biological baseline data for Quivira National Wildlife Refuge as of January 2016. It contains data on species found on the refuge, when and where...

  8. A comparison of blood gases, biochemistry, and hematology to ecomorphology in a health assessment of pinfish (Lagodon rhomboides

    Directory of Open Access Journals (Sweden)

    Sara Collins


    Full Text Available Despite the promise of hematological parameters and blood chemistry in monitoring the health of marine fishes, baseline data is often lacking for small fishes that comprise central roles in marine food webs. This study establishes blood chemistry and hematological baseline parameters for the pinfish Lagodon rhomboides, a small marine teleost that is among the most dominant members of near-shore estuarine communities of the Atlantic Ocean and Gulf of Mexico. Given their prominence, pinfishes are an ideal candidate species to use as a model for monitoring changes across a wide range of near-shore marine communities. However, pinfishes exhibit substantial morphological differences associated with a preference for feeding in primarily sea-grass or sand dominated habitats, suggesting that differences in the foraging ecology of individuals could confound health assessments. Here we collect baseline data on the blood physiology of pinfish while assessing the relationship between blood parameters and measured aspects of feeding morphology using data collected from 37 individual fish. Our findings provide new baseline health data for this important near shore fish species and find no evidence for a strong linkage between blood physiology and either sex or measured aspects of feeding morphology. Comparing our hematological and biochemical data to published results from other marine teleost species suggests that analyses of trends in blood value variation correlated with major evolutionary transitions in ecology will shed new light on the physiological changes that underlie the successful diversification of fishes.

  9. The Monitoring of Some Blood Physiological and Biochemical Index of Cows During Perinatal Period%围产期奶牛几种血液生理生化指标监测

    Institute of Scientific and Technical Information of China (English)

    李俊杰; 申国新; 汪恩强; 安锡忠; 陨祺


    In order to monitor and diagnose common perinatal cow nutrition and metabolic diseases,totally 60 perinatal cows (10 cows respectively in prenatal 15d, prenatal 7d, prenatal 1d, postpartum 1d, postpartum 7d, postpartum 15d) were selected for physiological and biochemical blood testing. The experimental results showed that:all transition cows white blood cell count, red blood cell count, hemoglobin and hematocrit of blood were little changed. It can not be served as the basis for monitoring of nutritional and metabolic diseases on transition cows. the minimum content of prenatal 1d cow serum free fatty acid was the lowest (460.76±268.34)μmol/L, in which respectively having the signiifcant differences (P<0.01) among prenatal 15d, postpartum 7d, 15d postpartum. The minimum content of postpartum cow serum glucose was the lowest (2.12±0.35)mmol/L, in which respectively compared with other times measured values were significantly different (P<0.01). The maximum content of postpartum 1d cow serum aspartate aminotransferase was the highest (15.33±3.24)U/L, in which respectively having the signiifcant differences(P<0.01)among prenatal 15d and prenatal 7d. 15d postpartum cow serum alanine aminotransferase activity and triglyceride levels were elevated (14.10 ± 4.40) U/L and (0.90 ± 0.14) mmol / L, compared with prenatal different periods were significantly different (P<0.01). The minimize content of Ca in the serum of postpartum 1d down to(1.76±0.32)mmol/L, serum hydroxyproline content and alkaline phosphatase activity were elevated (25.50 ± 7.90)μmol/L and (85.13±7.57) IU/L respectively, having signiifcant difference with prenatal(P<0.01). Conclusion:Using serum FFA, serum GLU and serum AST, and according to formula calculate Y as a basis for the diagnosis of fatty liver cows. The content of cow serum Ca was decreased. The content of serum HYP and activity increasing of serum ALP serve as the basis for the diagnosis of malnutrition cow bone. Ketone monitoring

  10. Biochemical values in free-ranging white rhinoceros (Ceratotherium simum) in Kruger National Park, South Africa. (United States)

    Mathebula, Nomkhosi; Miller, Michele; Buss, Peter; Joubert, Jennifer; Martin, Laura; Kruger, Marius; Hofmeyr, Markus; Olea-Popelka, Francisco


    Biochemical panels were analyzed on 181 individual free-ranging white rhinoceros (Ceratotherium simum) from Kruger National Park, South Africa. These animals were immobilized between July 2006 and May 2010 for management purposes. Serum and heparinized plasma samples were analyzed using an in-house chemistry analyser (ABAXIS VetScan2). The objectives of this study were to establish biochemical references ranges for Kruger National Park's population of white rhinoceros; to assess differences in values obtained using sera or plasma; and to assess differences in values between gender and different age categories. Significant differences between plasma and serum values were found in most measured parameters except minerals (calcium and magnesium). Because all animals appeared clinically healthy at the time of blood collection, it is hypothesized that choice of anticoagulant may affect certain parameters. Comparison between age categories and gender also resulted in significant differences in a few measured parameters. Identifying differences are important when establishing baseline reference ranges for wildlife populations to allow accurate monitoring of trends that may change over time. The paucity of data on normal biochemical ranges for free-ranging white rhinoceros demonstrates the value of this study and importance of evaluating potential confounding variables.

  11. Establishment of baseline haematology and biochemistry parameters in wild adult African penguins (Spheniscus demersus

    Directory of Open Access Journals (Sweden)

    Nola J. Parsons


    Full Text Available There are few publications on the clinical haematology and biochemistry of African penguins (Spheniscus demersus and these are based on captive populations. Baseline haematology and serum biochemistry parameters were analysed from 108 blood samples from wild, adult African penguins. Samples were collected from the breeding range of the African penguin in South Africa and the results were compared between breeding region and sex. The haematological parameters that were measured were: haematocrit, haemoglobin, red cell count and white cell count. The biochemical parameters that were measured were: sodium, potassium, chloride, calcium, inorganic phosphate, creatinine, cholesterol, serum glucose, uric acid, bile acid, total serum protein, albumin, aspartate transaminase and creatine kinase. All samples were serologically negative for selected avian diseases and no blood parasites were detected. No haemolysis was present in any of the analysed samples. Male African penguins were larger and heavier than females, with higher haematocrit, haemoglobin and red cell count values, but lower calcium and phosphate values. African penguins in the Eastern Cape were heavier than those in the Western Cape, with lower white cell count and globulin values and a higher albumin/globulin ratio, possibly indicating that birds are in a poorer condition in the Western Cape. Results were also compared between multiple penguin species and with African penguins in captivity. These values for healthy, wild, adult penguins can be used for future health and disease assessments.

  12. Establishment of baseline haematology and biochemistry parameters in wild adult African penguins (Spheniscus demersus). (United States)

    Parsons, Nola J; Schaefer, Adam M; van der Spuy, Stephen D; Gous, Tertius A


    There are few publications on the clinical haematology and biochemistry of African penguins (Spheniscus demersus) and these are based on captive populations. Baseline haematology and serum biochemistry parameters were analysed from 108 blood samples from wild, adult African penguins. Samples were collected from the breeding range of the African penguin in South Africa and the results were compared between breeding region and sex. The haematological parameters that were measured were: haematocrit, haemoglobin, red cell count and white cell count. The biochemical parameters that were measured were: sodium, potassium, chloride, calcium, inorganic phosphate, creatinine, cholesterol, serum glucose, uric acid, bile acid, total serum protein, albumin, aspartate transaminase and creatine kinase. All samples were serologically negative for selected avian diseases and no blood parasites were detected. No haemolysis was present in any of the analysed samples. Male African penguins were larger and heavier than females, with higher haematocrit, haemoglobin and red cell count values, but lower calcium and phosphate values. African penguins in the Eastern Cape were heavier than those in the Western Cape, with lower white cell count and globulin values and a higher albumin/globulin ratio, possibly indicating that birds are in a poorer condition in the Western Cape. Results were also compared between multiple penguin species and with African penguins in captivity. These values for healthy, wild, adult penguins can be used for future health and disease assessments.

  13. Blood Types (United States)

    ... maternity. Learn About Blood Blood Facts and Statistics Blood Components Whole Blood and Red Blood Cells Platelets Plasma ... About Blood Blood Facts and Statistics Blood Types Blood Components What Happens to Donated Blood Blood and Diversity ...

  14. Comparative study on the blood biochemical indices of primiparous and multiparous dairy cows in perinatal stage%一胎奶牛与经产奶牛围产期血液生化指标对比研究

    Institute of Scientific and Technical Information of China (English)

    于洪江; 沈泰钰; 高三思; 黄宝银; 董志昊; 刘润琪; 何平; 李蕊蕊; 杨威


    The purpose of this study was to reflect the regular pattern of changes and differences in the blood biochemical indices between primiparous dairy cows and multiparous dairy cows.Five primiparous and five multiparous healthy dairy cows with similar body condition,year and parity were selected.Blood samples were collected from the vena caudalis mediana using sodium heparin before feeding and milking in the morning at day 7,4,1 d prenatal and day 0,1,3,5,7,9,11,14,17,20 d postpartum,and detecting the glucose (GLU),calcium (Ca),phosphorus (P) and magnesium (Mg),β-hydroxybutyrate (BHBA),free fatty acid (NEFA),total protein (TP),albumin (ALB),globulin (GLO),triglyceride (TG) in the blood.The results showed that there are significant differences in the concentration of GLU between both dairy cows at day 7,4 prenatal (P<0.01) and at 0,3,5,7,9,11,14,17,20 d postpartum(P<0.05).The GLU content reached a peak at the parturition.The concentration of Ca in the primiparous dairy cows is significantly higher than the multiparous dairy cows at day 0,1 postpartum (P<0.01),and significantly increased at day 4,1 prenatal (P<0.05),reaching the lowest at day 0.There are significantly difference in the phosphorus content between both at 0 d (P<0.01),phosphorus content in primiparous was higher than multiparous.The concentration of Mg in the primiparous was significantly higher than multiparous at day 1 postpartum (P<0.05).There is no significant difference in the concentrations of BHBA、NEFA、TG、TP、ALB、GLO between both (P> 0.05),and the concentrations of BHBA,NEFA,TP,ALB,GLO in multiparous dairy cows were slightly higher than primiparous.The results indicate that the Ca,P,Mg and energy metabolism of primiparous dairy cows are better than those of multiparous dairy cows,and there is a higher risk of the nutritional and metabolic diseases after parturition of multiparous dairy cows.%本试验旨在通过对围产期一胎奶牛与经产奶牛血液进行生化指

  15. Baseline Removal From EMG Recordings (United States)


    a time-varying baseline contamination. Acknowledgements: Work funded by the Departamento de Salud del Gobierno de Navarrra and by a Spanish MEC...Name(s) and Address(es) Departamento de Ingenieria Electra y Electronica Universidad Publica de Navarra Pamplona, Spain Performing Organization Report

  16. Biochemical response to lamivudine treatment in HBeAg negative chronic hepatitis B patients in Iran

    Institute of Scientific and Technical Information of China (English)

    Amir Houshang Mohammad Alizadeh; Mitra Ranjbar; Babak Karimi; Saeed Hatami


    AIM: To study the effect of a one-year lamivudine regimen in patients with chronic hepatitis B.METNODS: Medical records of HBeAg negative hepatitis B patients who attended a hepatitis clinic in Tehran between March 2002-March 2004 were evaluated. The patients received 100 mg lamivudine tablets once daily for at least 12 mo. Liver enzymes and complete blood count were checked at baseline and the end of treatment (12th mo) and 6 mo after discontinuation of treatment.RESULTS: Of all patients, 24 were excluded. Of 71patients left, 58 (81.7%) were men. Mean age of the patients was 38 ± 14 years. Mean level of ALT in serum was 1437 ± 205 nkat/L at baseline with a significant reduction at the end of treatment to a mean level of 723± 92 nkat/L (P = 0.002). In 38 patients (53.5%), the ALT level was normal after one-year treatment. Five patients (7.3%) relapsed (biochemically) within 6 mo after discontinuing lamivudine therapy (the patients with good end of treatment response). Mean level of AST in serum was 1060 ± 105 nkat/L at baseline which decreased significantly to 652 ± 75 nkat/L at the end of treatment (P = 0.002).CONCLUSION: Over half (53.5%) of chronic hepatitis B patients with HBeAg negative have normal liver enzyme level at 12-mo lamivudine therapy.

  17. Biochemical Abnormalities in Batten's Syndrome

    DEFF Research Database (Denmark)

    Clausen, Jytte Lene; Nielsen, Gunnar Gissel; Jensen, Gunde Egeskov;


    The present data indicate that a group of ten patients with Batten's syndrome showed reduced activity of erythrocyte glutathione (GSH) peroxidase (Px) (glutathione: H2O2 oxidoreductase, EC using H2O2 as peroxide donor. Assay of erythrocyte GSHPx using H2O2, cumene hydroperoxide and t......-butyl hydroperoxide as donors also makes it possible biochemically to divide Batten's syndrome into two types: (1) one type with decreased values when H2O2 and cumene hydroperoxide are used, and (2) one type with increased values when t-butyl hydroperoxide is used. Furthermore an increased content of palmitic, oleic...... in whole blood. In normal human beings a connection was found between the erythrocyte selenium content and GSHPx activity assayed by cumene hydroperoxide as a peroxide donor....

  18. Mode S Baseline Radar Tracking. (United States)


    range units and 20 azimuth units) overlaying the position of the beacon reports. In the cases analyzed where beacon reports were not radar reinforced ...82/53 j~ C ~ 7 C _ _ _ _ _ _ 4. Title end Su.btitle 5. Neget at. November 1982 MDDE S BASELINE RADAR TRACKIN4G 6. Poelin Orgeuianti.. Cede ACT-100...Ground Clutter 33 Mode S/ARTS III 100-Scan False Radar Track Summary 74 34 Percent Beacon Radar Reinforcement 77 vii INTRODUCTION PURPOSE. The purpose of

  19. BLOOD SERUM BIOCHEMICAL CHARACTERISTICS OF HOLSTEIN ANIMALS IMPORTED FROM CANADA AS HEIFERS Биохимическая характеристика сыворотки крови голштинских животных завезенных из канады нетелями

    Directory of Open Access Journals (Sweden)

    Tuzov I. N.


    Full Text Available The article describes the dynamics of blood serum biochemical characteristics of Holstein first – calf heifers imported from Canada to Kuban company, Krasnodar region. The precise analysis of the blood serum parameters is given in the article, as well as their interconnection

  20. 应激对猪生产性能、行为及血液理化指标影响的研究进展%Research Progress on Effects of Stress on Production Performance,Behaviour and Blood Physiological-biochemical Indexes in Pigs

    Institute of Scientific and Technical Information of China (English)

    杨培歌; 顾宪红


    Significant changes of production performance,behaviour and blood physiological-biochemical indexes were found under stress conditions, and stress could damage animals. Thus,it was suggested that every efforts should be made to prevent stress,especially severe stress in livestock production,so as not to affect the orderly production,damage animals welfare and reduce economic benefits. The influence of weaning,cold,heat,transportation and slaughter stress on production performance,behaviour and blood physiological-biochemical indexes in pigs were mainly reviewed in this paper.%应激条件下会影响猪的生产性能、行为及血液生理生化指标,对动物机体造成危害.因此,在畜牧生产实践中应尽一切努力防止应激特别是严重应激的发生,以免影响生产的有序进行,损害动物福利,降低经济效益.作者主要综述了断奶、冷、热、运输、屠宰等常见应激因素对猪的生产性能、行为及血液生理生化指标的影响.

  1. 全棉籽对奶牛生产性能及血清生化指标的影响%Effects of Whole Cottonseed on Performance and Blood Biochemical Paramaters of Dairy Cows

    Institute of Scientific and Technical Information of China (English)

    钟荣珍; 高艳霞; 曹玉凤; 李秋凤; 李建国


    This experiment was conducted to study the effects of dietary whole cottonseed (WCS) on performance and blood biochemical paramaters of dairy cows. A 4 × 4 Latin square design was used. Eight multiparity Holstein cows in early lactation were selected and assigned randomly into 4 groups with 2 replicates in each group and 1 cow per replicate. Cows in group A (control group) were fed a basal diet, and cows in groups B, C and D were fed experimental diets contained 5% , 10% and 15% WCS, respectively. The experiment contained four period, and each period lasted for 28 d with 7-d adaptation period and 21-d formality period. The results showed as follows: 1) with the increasing of WCS percentage, milk yield of dairy cows tended to be increased (P>0.05), and the milk fat percentage and 4% fat corrected milk (FCM) was significantly increased (P 0. 05). 2) Dietary WCS level had no effects on the apparent digestibilities of crude protein , ether extract and acid detergent fiber ( P > 0.05) , but it significantly increased the apparent digestibility of neutral detergent fiber (P 0. 05) , but it significantly affected the contents of cholesterol and urea nitrogen in serum (P 0. 05) , In conclusion, for dairy cows in early lactation period, the optimal percentage of WCS in the diet is 10% .%本试验旨在研究饲粮中添加全棉籽对奶牛生产性能及血清生化指标影响.试验采用4×4拉丁方设计,选择8头泌乳早期的经产中国荷斯坦奶牛,随机分为4组,每组2个重复,每个重复1头牛.A组为对照组,饲喂基础饲粮;B、C和D组分别饲喂含5%、10%和15%全棉籽的试验饲粮.共进行4期试验,每期试验28 d,其中预试期7d,正试期21 d.结果表明:1)随着全棉籽比例的增加,奶牛产奶量有提高趋势,奶牛的乳脂率和4%标准乳产量显著提高(P<0.01),但全棉籽对乳蛋白率、乳糖率和乳非脂固形物率无显著影响(P>0.05).2)全棉籽对粗蛋白质、粗脂肪、酸性洗涤纤

  2. Haematological and biochemical analysis in canine enteritis

    Directory of Open Access Journals (Sweden)

    Abid Ali Bhat

    Full Text Available Aim: The present investigation screened eighteen clinical cases of canine enteritis for haematological and biochemical analyses. Materials and Methods: Eighteen dogs suffering from enteritis were selected and detailed clinical manifestations were noted. Hematological and biochemical parameters were estimated by using various kits. Blood was also collected from twelve healthy dogs for establishing control values and data obtained were subjected to statistical analysis. Results: The affected dogs showed anorexia, diarrhoea, depression, varying degree of dehydration and tachycardia. There were significant changes in packed cell volume, neutrophils, lymphocytes and mean corpuscular haemoglobin concentration. Biochemical investigation revealed significant decrease in plasma glucose, total plasma protein, albumin and albumin:globulin ratio (A:G ratio. The level of potassium and chloride was markedly decreased. Significant increase in alanine aminotransferase (ALT and blood urea nitrogen (BUN was observed. Conclusion: Packed Cell Volume (PCV and Total Erythrocyte Count (TEC remained almost similar between healthy dogs and dogs affected with diarrhoea. Mean Total Leukocyte Count (TLC value was significantly higher as compared to the control group. Hypoglycemia, hypoproteinemia, hypokalemia, hypochloremia and increase in blood urea nitrogen was observed in dogs suffering from enteritis. [Vet World 2013; 6(7.000: 380-383

  3. Variability in baseline laboratory measurements of the Brazilian Longitudinal Study of Adult Health (ELSA-Brasil). (United States)

    Ladwig, R; Vigo, A; Fedeli, L M G; Chambless, L E; Bensenor, I; Schmidt, M I; Vidigal, P G; Castilhos, C D; Duncan, B B


    Multi-center epidemiological studies must ascertain that their measurements are accurate and reliable. For laboratory measurements, reliability can be assessed through investigation of reproducibility of measurements in the same individual. In this paper, we present results from the quality control analysis of the baseline laboratory measurements from the ELSA-Brasil study. The study enrolled 15,105 civil servants at 6 research centers in 3 regions of Brazil between 2008-2010, with multiple biochemical analytes being measured at a central laboratory. Quality control was ascertained through standard laboratory evaluation of intra- and inter-assay variability and test-retest analysis in a subset of randomly chosen participants. An additional sample of urine or blood was collected from these participants, and these samples were handled in the same manner as the original ones, locally and at the central laboratory. Reliability was assessed with the intraclass correlation coefficient (ICC), estimated through a random effects model. Coefficients of variation (CV) and Bland-Altman plots were additionally used to assess measurement variability. Laboratory intra and inter-assay CVs varied from 0.86% to 7.77%. From test-retest analyses, the ICCs were high for the majority of the analytes. Notably lower ICCs were observed for serum sodium (ICC=0.50; 95%CI=0.31-0.65) and serum potassium (ICC=0.73; 95%CI=0.60-0.83), due to the small biological range of these analytes. The CVs ranged from 1 to 14%. The Bland-Altman plots confirmed these results. The quality control analyses showed that the collection, processing and measurement protocols utilized in the ELSA-Brasil produced reliable biochemical measurements.

  4. Long-Baseline Neutrino Experiments (United States)

    Diwan, M. V.; Galymov, V.; Qian, X.; Rubbia, A.


    We review long-baseline neutrino experiments in which neutrinos are detected after traversing macroscopic distances. Over such distances neutrinos have been found to oscillate among flavor states. Experiments with solar, atmospheric, reactor, and accelerator neutrinos have resulted in a coherent picture of neutrino masses and mixing of the three known flavor states. We summarize the current best knowledge of neutrino parameters and phenomenology, with a focus on the evolution of the experimental technique. We proceed from the first evidence produced by astrophysical neutrino sources to the current open questions and the goals of future research.

  5. Long-Baseline Neutrino Experiments

    CERN Document Server

    Diwan, M V; Qian, X; Rubbia, A


    We review long-baseline neutrino experiments in which neutrinos are detected after traversing macroscopic distances. Over such distances neutrinos have been found to oscillate among flavor states. Experiments with solar, atmospheric, reactor, and accelerator neutrinos have resulted in a coherent picture of neutrino masses and mixing of the three known flavor states. We will summarize the current best knowledge of neutrino parameters and phenomenology with our focus on the evolution of the experimental technique. We proceed from the first evidence produced by astrophysical neutrino sources to the current open questions and the goals of future research.

  6. Baseline LAW Glass Formulation Testing

    Energy Technology Data Exchange (ETDEWEB)

    Kruger, Albert A. [USDOE Office of River Protection, Richland, WA (United States); Mooers, Cavin [The Catholic University of America, Washington, DC (United States). Vitreous State Lab.; Bazemore, Gina [The Catholic University of America, Washington, DC (United States). Vitreous State Lab; Pegg, Ian L. [The Catholic University of America, Washington, DC (United States). Vitreous State Lab; Hight, Kenneth [The Catholic University of America, Washington, DC (United States). Vitreous State Lab; Lai, Shan Tao [The Catholic University of America, Washington, DC (United States). Vitreous State Lab; Buechele, Andrew [The Catholic University of America, Washington, DC (United States). Vitreous State Lab; Rielley, Elizabeth [The Catholic University of America, Washington, DC (United States). Vitreous State Lab; Gan, Hao [The Catholic University of America, Washington, DC (United States). Vitreous State Lab; Muller, Isabelle S. [The Catholic University of America, Washington, DC (United States). Vitreous State Lab; Cecil, Richard [The Catholic University of America, Washington, DC (United States). Vitreous State Lab


    The major objective of the baseline glass formulation work was to develop and select glass formulations that are compliant with contractual and processing requirements for each of the LAW waste streams. Other objectives of the work included preparation and characterization of glasses with respect to the properties of interest, optimization of sulfate loading in the glasses, evaluation of ability to achieve waste loading limits, testing to demonstrate compatibility of glass melts with melter materials of construction, development of glass formulations to support ILAW qualification activities, and identification of glass formulation issues with respect to contract specifications and processing requirements.

  7. Measures of Biochemical Sociology (United States)

    Snell, Joel; Marsh, Mitchell


    In a previous article, the authors introduced a new sub field in sociology that we labeled "biochemical sociology." We introduced the definition of a sociology that encompasses sociological measures, psychological measures, and biological indicators Snell & Marsh (2003). In this article, we want to demonstrate a research strategy that would assess…

  8. Biochemical Education in Brazil. (United States)

    Vella, F.


    Described are discussions held concerning the problems of biochemical education in Brazil at a meeting of the Sociedade Brazileira de Bioquimica in April 1988. Also discussed are other visits that were made to universities in Brazil. Three major recommendations to improve the state of biochemistry education in Brazil are presented. (CW)

  9. FED baseline engineering studies report

    Energy Technology Data Exchange (ETDEWEB)

    Sager, P.H.


    Studies were carried out on the FED Baseline to improve design definition, establish feasibility, and reduce cost. Emphasis was placed on cost reduction, but significant feasibility concerns existed in several areas, and better design definition was required to establish feasibility and provide a better basis for cost estimates. Design definition and feasibility studies included the development of a labyrinth shield ring concept to prevent radiation streaming between the torus spool and the TF coil cryostat. The labyrinth shield concept which was developed reduced radiation streaming sufficiently to permit contact maintenance of the inboard EF coils. Various concepts of preventing arcing between adjacent shield sectors were also explored. It was concluded that installation of copper straps with molybdenum thermal radiation shields would provide the most reliable means of preventing arcing. Other design studies included torus spool electrical/structural concepts, test module shielding, torus seismic response, poloidal conditions in the magnets, disruption characteristics, and eddy current effects. These additional studies had no significant impact on cost but did confirm the feasibility of the basic FED Baseline concept.

  10. Blood gases, biochemistry, and hematology of Galapagos green turtles (Chelonia mydas.

    Directory of Open Access Journals (Sweden)

    Gregory A Lewbart

    Full Text Available The green turtle, Chelonia mydas, is an endangered marine chelonian with a circum-global distribution. Reference blood parameter intervals have been published for some chelonian species, but baseline hematology, biochemical, and blood gas values are lacking from the Galapagos sea turtles. Analyses were done on blood samples drawn from 28 green turtles captured in two foraging locations on San Cristóbal Island (14 from each site. Of these turtles, 20 were immature and of unknown sex; the other eight were males (five mature, three immature. A portable blood analyzer (iSTAT was used to obtain near immediate field results for pH, lactate, pO2, pCO2, HCO3-, Hct, Hb, Na, K, iCa, and Glu. Parameter values affected by temperature were corrected in two ways: (1 with standard formulas; and (2 with auto-corrections made by the iSTAT. The two methods yielded clinically equivalent results. Standard laboratory hematology techniques were employed for the red and white blood cell counts and the hematocrit determination, which was also compared to the hematocrit values generated by the iSTAT. Of all blood analytes, only lactate concentrations were positively correlated with body size. All other values showed no significant difference between the two sample locations nor were they correlated with body size or internal temperature. For hematocrit count, the iSTAT blood analyzer yielded results indistinguishable from those obtained with high-speed centrifugation. The values reported in this study provide baseline data that may be useful in comparisons among populations and in detecting changes in health status among Galapagos sea turtles. The findings might also be helpful in future efforts to demonstrate associations between specific biochemical parameters and disease.

  11. Blood gases, biochemistry, and hematology of Galapagos green turtles (Chelonia mydas). (United States)

    Lewbart, Gregory A; Hirschfeld, Maximilian; Denkinger, Judith; Vasco, Karla; Guevara, Nataly; García, Juan; Muñoz, Juanpablo; Lohmann, Kenneth J


    The green turtle, Chelonia mydas, is an endangered marine chelonian with a circum-global distribution. Reference blood parameter intervals have been published for some chelonian species, but baseline hematology, biochemical, and blood gas values are lacking from the Galapagos sea turtles. Analyses were done on blood samples drawn from 28 green turtles captured in two foraging locations on San Cristóbal Island (14 from each site). Of these turtles, 20 were immature and of unknown sex; the other eight were males (five mature, three immature). A portable blood analyzer (iSTAT) was used to obtain near immediate field results for pH, lactate, pO2, pCO2, HCO3-, Hct, Hb, Na, K, iCa, and Glu. Parameter values affected by temperature were corrected in two ways: (1) with standard formulas; and (2) with auto-corrections made by the iSTAT. The two methods yielded clinically equivalent results. Standard laboratory hematology techniques were employed for the red and white blood cell counts and the hematocrit determination, which was also compared to the hematocrit values generated by the iSTAT. Of all blood analytes, only lactate concentrations were positively correlated with body size. All other values showed no significant difference between the two sample locations nor were they correlated with body size or internal temperature. For hematocrit count, the iSTAT blood analyzer yielded results indistinguishable from those obtained with high-speed centrifugation. The values reported in this study provide baseline data that may be useful in comparisons among populations and in detecting changes in health status among Galapagos sea turtles. The findings might also be helpful in future efforts to demonstrate associations between specific biochemical parameters and disease.

  12. 甘草黄酮对2型糖尿病大鼠血糖、血脂等生化指标的影响%Effect of licorice flavonoids on blood glucose, blood lipid and other biochemical indicators in type 2 diabetic rats

    Institute of Scientific and Technical Information of China (English)

    赵海燕; 王勇; 吴力武; 马永平


    Objective To study the effect of licorice flavonoids (LF) on fasting blood levels of glucose (FBG), lipids, insulin, adipokines and other biochemical indicators. Methods The type 2 diabetic rat model was replicated by feeding high-fat and high-sugar diet combined with low-dose streptozotocin (STZ) injection. The diabetic rats were divided into model group (DM), high-dose LF treatment group (HLF) and low-dose LF treatment group (LLF). Other 20 normal rats were divided into control group (Con) and control treatment group (CLF). Rats in different groups were administered LF (lOOmg or 300mg ? Kg"1 ? D"1) or solvent (propylene glycol) respectively. Five weeks later, the levels of fasting levels of glucose (FBG), triglycerides (TG), total cholesterol (TO, free fatty acids (FFA), high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol (LDL-C) , total antioxidation capability (T-AOC), insulin (Ins), tumor necrosis factor alpha (TNF-a) and leptin were examined. Insulin sensitivity index (ISI), liver index, kidney index and the relative weight of abdominal adipose tissue were calculated. Results The high-dose LF (300mg ? Kg"1 ? D~' ) treatment significantly decreased the levels of FBG, TG, TC, LDL-C, FFA, T-AOC, TNF-a, leptin, liver index and kidney index, and increased the levels of T-AOC, ISI and the relative weight of abdominal adipose tissue. Compared with high-dose treatment, low dose of LF was less effective. Conclusion LF significantly decreases the blood glucose level, improves the lipid metabolic disorders and ameliorates insulin resistance in type 2 diabetic rats.%目的 研究甘草黄酮(LF)对T2DM大鼠血糖、血脂等生化指标的影响.方法 将实验大鼠分为模型组(DM)、高剂量给药组(HLF)、低剂量给药组(LLF)、正常对照组(Con)和正常给药组(CLF).分别以LF或溶剂灌胃,5周后检测实验大鼠的空腹血糖、血脂等生化指标及肝体比、肾体比和脂体比.结果 高剂量LF(300 mg·kg-1

  13. Acute effects of vinegar intake on some biochemical risk factors of atherosclerosis in hypercholesterolemic rabbits

    Directory of Open Access Journals (Sweden)

    rohani Ali


    Full Text Available Abstract Background Exaggerated postprandial spikes in blood glucose and lipids induce proportional increases in oxidative stress, which acutely trigger impairment endothelial, inflammation and increased risk of future cardiovascular events. In this research, we have investigated acute effects of vinegar intake on some of the biochemical atherosclerosis risk factors in high cholesterol fed rabbits to see if we can find a probable protective value for it. Methods The rabbits were randomly divided into four groups: normal diet, high cholesterol diet (%1cholesterol, %1 cholesterol with 5 ml vinegar (low dose, %1 cholesterol with 10 ml vinegar (high dose. After fasting for 12-15 hours, blood samples were taken to determine baseline values. Three hours after feeding, blood samples were collected again to investigate acute effects of vinegar intake on the measured factors. Results Using high-dose vinegar with cholesterolemic diet caused significant reduce in LDL-cholesterol (LDL-C, oxidized-LDL (ox-LDL, malondialdehyde (MDA, total cholesterol (TC and apolipoprotein B (ApoB in comparison with hypercholesterolemic diet. Consumption low-dose vinegar with cholesterolemic diet induced a significant decrease in fibrinogen and glucose compared to hypercholesterolemic diet. Level of serum nitrite, nitrate, triacylglycerol (TAG, HDL-cholesterol (HDL-C, apolipoprotein A (ApoA, serum glutamic pyruvic transaminase (SGPT, serum glutamic oxaloacetate transaminase (SGOT and C-reactive protein (CRP were not significantly difference in low and high doses vinegar with cholesterolemic diet compared to hypercholesterolemic diet. A significant difference was observed for LDL-C, ApoB100 and TC between low and high doses vinegar. Conclusion This study suggest that vinegar, might have some acute effects on biochemical risk factors of atherosclerosis and a probable protective value can be considered for its postprandial use.

  14. Evaluation of the Effect of High Blood Fat Elimination by Alpha Cyclodextrin to the Clinical Biochemical Measure-ment%α-环糊精消除高脂血对临床生化测定影响的效果评价

    Institute of Scientific and Technical Information of China (English)

    朱征; 杨敏


    目的:分析高脂血对临床生化测定的影响,探讨利用α-环糊精(α-CD)消除高脂血对常用临床生化指标测定的影响。方法使用α-环糊精处理正常对照组、模拟脂血组、患者脂血组血清,使用OLYMPUS AU-640全自动生化分析仪分别测定处理前后临床常用生化指标,将测定结果进行统计学分析。临床可接受性能判断以美国临床实验室修正案(CLIA’88)允许总误差(TE a)的1/2为标准,判断其偏差是否为临床可接受。结果①正常对照组与模拟脂血组比较,除ALP、GGT、LDH、GLU、CK、α-AMY的平均偏差低于1/2TE a外,其余常用临床生化指标的平均偏差均高于1/2 TE a;②正常对照组、模拟脂血组使用α-CD处理前后,除TP、α-AMY、CHO、TG的平均偏差高于1/2TE a外,其余常用临床生化指标的平均偏差均低于1/2 TE a;③患者脂血组血清经α-CD处理后,其常用临床生化指标测定结果明显得到改善,其测定值更接近真实值。结论高脂血影响大部分临床常用生化指标测定的准确性,使用α-环糊精可以消除高脂血对大部分临床常用生化检测指标测定结果的影响。%Objective To analyze the effect of high fat blood on clinical biochemical measurement, and to explore the effect of the alpha-cyclodextrin(-CD) on the determination of common clinical biochemical indexes. Methods The serum of nor-mal control group,simulated lipid group,patient blood lipid group was processed by Alpha cyclodextrin. AU-640 OLYMPUS auto-matic biochemical analyzer was used before and after the treatment to determine the clinical biochemical indicators,the results were statistically analyzed. Clinical acceptable judgment in the United States clinical laboratory amendment(CLIA’88)allows the total er-ror of 1/2 as standard( TE a) to determine whether the deviation is clinically acceptable. Results ①In the comparison of normal control group and simulated lipid

  15. Pinellas Plant Environmental Baseline Report

    Energy Technology Data Exchange (ETDEWEB)


    The Pinellas Plant has been part of the Department of Energy`s (DOE) nuclear weapons complex since the plant opened in 1957. In March 1995, the DOE sold the Pinellas Plant to the Pinellas County Industry Council (PCIC). DOE has leased back a large portion of the plant site to facilitate transition to alternate use and safe shutdown. The current mission is to achieve a safe transition of the facility from defense production and prepare the site for alternative uses as a community resource for economic development. Toward that effort, the Pinellas Plant Environmental Baseline Report (EBR) discusses the current and past environmental conditions of the plant site. Information for the EBR is obtained from plant records. Historical process and chemical usage information for each area is reviewed during area characterizations.

  16. TWRS privatization process technical baseline

    Energy Technology Data Exchange (ETDEWEB)

    Orme, R.M.


    The U.S. Department of Energy (DOE) is planning a two-phased program for the remediation of Hanford tank waste. Phase 1 is a pilot program to demonstrate the procurement of treatment services. The volume of waste treated during the Phase 1 is a small percentage of the tank waste. During Phase 2, DOE intends to procure treatment services for the balance of the waste. The TWRS Privatization Process Technical Baseline (PPTB) provides a summary level flowsheet/mass balance of tank waste treatment operations which is consistent with the tank inventory information, waste feed staging studies, and privatization guidelines currently available. The PPTB will be revised periodically as privatized processing concepts are crystallized.

  17. Integrated Baseline Review (IBR) Handbook (United States)

    Fleming, Jon F.; Kehrer, Kristen C.


    The purpose of this handbook is intended to be a how-to guide to prepare for, conduct, and close-out an Integrated Baseline Review (IBR). It discusses the steps that should be considered, describes roles and responsibilities, tips for tailoring the IBR based on risk, cost, and need for management insight, and provides lessons learned from past IBRs. Appendices contain example documentation typically used in connection with an IBR. Note that these appendices are examples only, and should be tailored to meet the needs of individual projects and contracts. Following the guidance in this handbook will help customers and suppliers preparing for an IBR understand the expectations of the IBR, and ensure that the IBR meets the requirements for both in-house and contract efforts.

  18. 贵妃鸡与麒麟鸡杂交F1代血液生理生化指标的测定%Measurement of Blood Physiological and Biochemical of hybrid F1 of Princess Chicken and Frizzle Chicken

    Institute of Scientific and Technical Information of China (English)

    李乃宾; 杨芬霞; 杜炳旺; 陈洁波; 陶林


    为给贵妃鸡(♂)与麒麟鸡(♀)杂交F1代的人工饲养繁殖、健康状况检测以及经济开发等提供科学的参考数据和理论依据,试验对12周龄贵妃鸡与麒麟鸡杂交F1代进行血液生理生化指标测定与分析。结果表明:①血液生理指标:杂交公鸡红细胞计数(RBC)、血红蛋白浓度(HGB)、红细胞压积(HCT)在数值上极显著高于母鸡(P<0.01),白血球计数(WBC)、平均红细胞体积(MCV)显著高于母鸡(P<0.05);②血液生化指标:杂交公鸡的甘油三酯(TG)、尿酸(UA)指标在数值上显著高于母鸡(P<0.05),磷酸肌酸激酶(CK)、肌酸激酶同工酶(CK-MB)指标在数值上显著低于母鸡(P<0.05)。杂交F1代的血液生理生化指标具有相对稳定性,这一结果能为贵妃鸡(♂)与麒麟鸡(♀)杂交F1代疾病诊断、治疗和饲养管理提供参考。%In order to provide scientific reference and theoretical basis for breeding, health testing and economic development of hybrid F1 of Princess Chicken and Frizzle Chicken, the blood physiological and biochemical indexes of 12-week-old F1 Hybrid Offspring of Princess Chicken(♂)and Frizzle Chicken (♀)were measured.The results showed that①The blood physiological indexes:12 weeks of F1 generation of hybrid cock red blood cell count (RBC), hemoglobin concentration (HGB)and RBC deposited (HCT) were very significantly higher than the hens (P<0.01), average white blood cell count (WBC) and red blood cell volume (MCV) were significantly higher than the hens (P<0.05);②The blo-od biochemical indexes:12 weeks of F1 generation of hybrid cock triglyceride (TG)and uric acid (UA) index were significantly higher than the hens (P<0.05), creatine phosphate kinase (CK)and creatine kinase isoenzyme (CK-MB) index were significantly lower than the henl (P<0.05).The blood biochemical indicators of 12-week-old hybrid chickens were relatively stable,this conclusion provided a certain theoretical

  19. Multiplexing oscillatory biochemical signals. (United States)

    de Ronde, Wiet; ten Wolde, Pieter Rein


    In recent years it has been increasingly recognized that biochemical signals are not necessarily constant in time and that the temporal dynamics of a signal can be the information carrier. Moreover, it is now well established that the protein signaling network of living cells has a bow-tie structure and that components are often shared between different signaling pathways. Here we show by mathematical modeling that living cells can multiplex a constant and an oscillatory signal: they can transmit these two signals simultaneously through a common signaling pathway, and yet respond to them specifically and reliably. We find that information transmission is reduced not only by noise arising from the intrinsic stochasticity of biochemical reactions, but also by crosstalk between the different channels. Yet, under biologically relevant conditions more than 2 bits of information can be transmitted per channel, even when the two signals are transmitted simultaneously. These observations suggest that oscillatory signals are ideal for multiplexing signals.

  20. Atlantic NAD 83 SLA Baseline Tangents (United States)

    Bureau of Ocean Energy Management, Department of the Interior — This data set contains baseline tangent lines in ArcGIS shapefile format for the BOEM Atlantic Region. Baseline tangent lines are typically bay or river closing...

  1. Atlantic NAD 83 SLA Baseline Points (United States)

    Bureau of Ocean Energy Management, Department of the Interior — This data set contains baseline points in ArcGIS shapefile format for the BOEM Atlantic Region. Baseline points are the discrete coordinate points along the...

  2. 2016 Annual Technology Baseline (ATB) - Webinar Presentation

    Energy Technology Data Exchange (ETDEWEB)

    Cole, Wesley; Kurup, Parthiv; Hand, Maureen; Feldman, David; Sigrin, Benjamin; Lantz, Eric; Stehly, Tyler; Augustine, Chad; Turchi, Craig; Porro, Gian; O' Connor, Patrick; Waldoch, Connor


    This deck was presented for the 2016 Annual Technology Baseline Webinar. The presentation describes the Annual Technology Baseline, which is a compilation of current and future cost and performance data for electricity generation technologies.

  3. Assessment of Hematological and Biochemical parameters with extended D-Ribose ingestion

    Directory of Open Access Journals (Sweden)

    Frelich Angela


    Full Text Available Abstract D-ribose, a naturally occurring pentose carbohydrate, has been shown to replenish high- energy phosphates following myocardial ischemia and high intensity, repetitive exercise. Human studies have mainly involved short-term assessment, including potential toxicity. Reports describing adverse effects of D-ribose with prolonged ingestion have been lacking. Therefore, this study assessed the toxicity of extended consumption of D-ribose in healthy adults. Nineteen subjects ingested 20 grams/Day (10 grams, twice a Day of ribose with serial measurements of biochemical and hematological parameters at Days 0, 7, and 14. No significant toxic changes over the 14-day assessment period occurred in complete blood count, albumin, alkaline phosphatase, gamma glutamyltransferase, alanine amiotransferase, and aspartate aminotransferase. However, D-ribose did produce an asymptomatic, mild hypoglycemia of short duration. Uric acid levels increased at Day 7, but decreased to baseline values by Day 14. D-ribose consumption for 14 days appears not to produce significant toxic changes in both hematological and biochemical parameters in healthy human volunteers.

  4. Blood sugar test - blood (United States)

    ... blood glucose level ( hypoglycemia ) may be due to: Hypopituitarism (a pituitary gland disorder) Underactive thyroid gland or ... tonic-clonic seizure Glucagon blood test Glucagonoma Hyperthyroidism Hypopituitarism Hypothyroidism Insulinoma Low blood sugar Multiple endocrine neoplasia ( ...

  5. The effect of tiaprofenic acid on blood pressure control in treated hypertensive patients. (United States)

    Smith, M D; Kupa, A; Weatherall, M; Henstridge, J D; Brooks, P M


    Eleven patients with osteoarthritis and mild hypertension completed an 8-week, double-blind crossover study in which 200 mg tiaprofenic acid 3-times daily or placebo were substituted for their normal non-steroidal anti-inflammatory therapy. Systolic blood pressure was significantly higher on tiaprofenic acid therapy than on placebo and plasma renin activity was significantly lower on active treatment. No significant changes were seen in biochemical parameters, though the weight of the patient was also higher on tiaprofenic acid than on placebo. Duration of morning stiffness was also lower on tiaprofenic acid than on placebo. Blood pressure on tiaprofenic acid was not different from baseline readings on other non-steroidal anti-inflammatory drug therapy. This study suggests that tiaprofenic acid, like other non-steroidal anti-inflammatory agents, may interfere with blood pressure control in treated hypertensive patients.

  6. 2016 Annual Technology Baseline (ATB)

    Energy Technology Data Exchange (ETDEWEB)

    Cole, Wesley; Kurup, Parthiv; Hand, Maureen; Feldman, David; Sigrin, Benjamin; Lantz, Eric; Stehly, Tyler; Augustine, Chad; Turchi, Craig; O' Connor, Patrick; Waldoch, Connor


    Consistent cost and performance data for various electricity generation technologies can be difficult to find and may change frequently for certain technologies. With the Annual Technology Baseline (ATB), National Renewable Energy Laboratory provides an organized and centralized dataset that was reviewed by internal and external experts. It uses the best information from the Department of Energy laboratory's renewable energy analysts and Energy Information Administration information for conventional technologies. The ATB will be updated annually in order to provide an up-to-date repository of current and future cost and performance data. Going forward, we plan to revise and refine the values using best available information. The ATB includes both a presentation with notes (PDF) and an associated Excel Workbook. The ATB includes the following electricity generation technologies: land-based wind; offshore wind; utility-scale solar PV; concentrating solar power; geothermal power; hydropower plants (upgrades to existing facilities, powering non-powered dams, and new stream-reach development); conventional coal; coal with carbon capture and sequestration; integrated gasification combined cycle coal; natural gas combustion turbines; natural gas combined cycle; conventional biopower. Nuclear laboratory's renewable energy analysts and Energy Information Administration information for conventional technologies. The ATB will be updated annually in order to provide an up-to-date repository of current and future cost and performance data. Going forward, we plan to revise and refine the values using best available information.

  7. Clinical Testing of Blood Glucose Meter Rapid Clinical Significance and Ap-plication of Conventional Biochemical Analyzer%刍议临床血糖检验中快速血糖仪与常规生化仪应用的临床意义

    Institute of Scientific and Technical Information of China (English)



    目的:研究快速血糖仪以及常规生化仪应用于临床血糖检验工作中发挥的效果。方法选择2013年5月-2014年5月该院68例患者,按每组34例分别设置对照组和观察组,对照组选择常规生化仪开展血糖检验工作,观察组通过快速血糖仪对患者血糖进行临床检验,比较两组检验结果。结果对照组患者最终测出的血糖值为(7.73±3.26)mmol/L;观察组患者最终的血糖检验值为(7.26±3.13)mmol/L,两组患者的检测结果差异无统计学意义(P>0.05)。结论针对患者的临床血糖检验工作,采用快速血糖仪以及常规生化仪所检出的结果不存在显著区别,应用效果不存在明显差异,但是快速血糖仪在检验操作上更为快捷和方便,应用优势较突出。%Objective To study the fast blood glucose meter and biochemical analyzer used in clinical routine blood test work, play effect. Methods From May 2013 to May 2014 in our hospital 68 patients, 34 cases in each group were set ac-cording to the control group and observation group and control group selection routine blood chemistry analyzer to carry out inspection work, study group patients by rapid blood glucose meter clinical blood test, the test results were compared. Re-sults The final group of patients measured blood glucose value (7.73 ± 3.26) mmol / L; Observation group were eventually glucose test is ( 7.26±3.13)mmol/ L, the test results of the two groups were not significantly different, not statistically sig-nificance (P>0.05). Conclusion For patients with clinical blood test work, using rapid blood glucose meter and convention-al biochemical analyzer results are detected significant differences do not exist, there is no significant difference in the ef-fect of the application, but a more rapid glucose meter quick and easy on the test operation, the application more prominent advantages.

  8. The Effects of Probiotic Supplementation on Markers of Blood Lipids, and Blood Pressure in Patients with Prediabetes: A Randomized Clinical Trial (United States)

    Mahboobi, Sepideh; Iraj, Bijan; Maghsoudi, Zahra; Feizi, Awat; Ghiasvand, Reza; Askari, Gholamreza; Maayeshi, Najmeh


    Background: Prediabetes is a high-risk condition for type 2 diabetes mellitus. The growing prevalence of diabetes emphasizes on the necessity of concentrating on various strategies to prediabetes prevention and management. Probiotics as a group of functional foods might exert antidiabetic effects. This study aimed to assess the effects of probiotic administration on blood lipid profile and blood pressure in patients with prediabetes. Methods: This randomized controlled trial consisted of 60 prediabetic patients, aged 25-65 years old, that were randomly assigned to the intervention (receiving 500 mg probiotic capsules, n = 30) or control group (receiving placebo, n = 30) for 8-week period. Demographic and anthropometric data were collected at baseline. Blood samples were collected at baseline and after 8 weeks for biochemical measurements. Blood pressure was measured at the baseline an after 8 weeks of intervention. Data regarding dietary intakes and physical activity were also collected during the study. We used SPSS software version 16 (SPSS Inc. Chicago, USA) for data analyzing. Results: Probiotic supplementation did not contribute to significant changes in total cholesterol, low-density lipoprotein (LDL)-cholesterol, high-density lipoprotein (HDL)-cholesterol, Triglycerides (TG), TG/LDL and LDL/HDL ratios, after 8 weeks. After adjusting for potential confounders, HDL-cholesterol reduced significantly in the placebo group compared with probiotic group. Percent change in systolic blood pressure was significantly different in the probiotic group in comparison with a placebo group (–3.10 ± 2.22 vs. 3.24 ± 1.96, P = 0.01), although this significance did not exist anymore after adjusting for confounders (P > 0.05). Conclusions: Our study showed that probiotics did not have significant effects on lipid markers although they had positive effects on systolic blood pressure. PMID:25400881

  9. 动机访谈路线图对糖尿病患者血生化指标及自我效能的影响%Influence of motivational interviewing roadmap on the blood biochemical indexe and the self-efficacy of diabetes patients

    Institute of Scientific and Technical Information of China (English)

    邓春友; 谢永康; 利友琼; 陈玉婷; 张远霞


    Objective To explore the influence of motivational interviewing roadmap on the blood biochemical indexe and the self-efficacy of diabetes patients. Methods 60 cases with diabetes from March 2012 to February 2013 in our hospital were selected as the control group,which was given traditional nursing.68 cases with diabetes from March 2013 to February 2014 in our hospital were selected as the research group,which was given nursing intervention of motiva-tional interviewing roadmap on the basis of the control group.The blood biochemical index,self-efficacy score and score of six dimensions in two groups was compared. Results The level of fasting blood glucose,postprandial 2 h blood glu-cose,glycosylated hemoglobin and BMI in the research group after intervention was lower than that in the control group, with significant difference (P<0.05).The various self-efficacy scores of the study group after intervention was higher than that of the control group,with significant difference (P<0.05). Conclusion The application of the motivational interview route map in the patients with diabetes can improve the blood biochemical parameters and improve the self-efficacy, and can achieve good economic benefits and social benefits,it is worthy of clinical promotion and application.%目的:动机访谈路线图对糖尿病患者血生化指标及自我效能的影响。方法选取本院2012年3月~2013年2月收治的60例糖尿病患者作为对照组,实施传统护理方案。选取本院2013年3月~2014年2月收治的68例糖尿病患者作为研究组,在对照组的基础上开展动机访谈路线图进行护理干预。比较两组干预前后的血生化指标、自我效能总分及6个维度得分。结果研究组干预后的空腹血糖、餐后2 h血糖、糖化血红蛋白及BMI水平显著低于对照组,差异有统计学意义(P<0.05)。研究组干预后的各项自我效能评分显著高于对照组,差异有统计学意义(P<0.05)。结论在糖尿病

  10. 实验性上腹器官簇移植术中血流动力学及生化指标的变化%Hemodynamic and blood biochemical changes in experimental transplantation of abdominal organ cluster

    Institute of Scientific and Technical Information of China (English)

    詹文华; 吴伍林; 蔡世荣; 陈规划; 黑子清; 何小顺; 邓天忠


    Intraoperative and postoperative changes of physiologic items in abdominal organ cluster transplantation in 14 recipients out-bred pigs.Mean cold ischemia time was 154±26 min, mean bypass time 64±13 min.Mean total operative time was 222±31 min, mean intraoperative blood loss was 1600±210 ml and 935±65 ml blood was infused in the recipients.Mean body tem-perature was 33.1±1.83℃, decreased 3±1.53℃20 min after operation.Postoperative changes of blood pH, HCO3, PaC02 revealed that metabolic acidosis occurred in most of the recipients(10/14).Of them 8 recipients had respiratory compensation.The blood PaO2 and oxygen saturation in 11 recipients were monitored without significant changes, whereas blood oxygen content declined.In 3 recipients, hyperkalemia occurred as the transplants were perfused only with Collins solution without being flushed.Compared with hemodynamic items 20 min after pre-anhepatic operation began, mean arterial pressure(MAP), central venous pressure(CVP), and cardiac output(CO) at the early time of bypass and opening of blood vessels had significant changes.It was believed that the key to perform operation successfully was to reduce intraoperative bleeding, maintain nor-mal body temperature, balance of acid-base, and hemodynamics.%报告14例杂种猪上腹部器官簇移植的术中、术后生理学指标变化.平均冷缺血时间为154±26分钟,平均转流时间64±13分钟.受体平均手术时间222±31分钟.受体术中平均失血1600±210ml,平均输血935±65ml.手术结束后20分钟平均体温33.14±1.83℃,下降3±1.53℃.手术前后血pH、HCO-3及动脉血二氧化碳分压(PaCO2)指标改变显示大部分受体(10/14)术后出现代谢性酸中毒,8例有呼吸性代偿.所测11例受体手术前后动脉血氧分压(PaO2)、血氧饱和度无明显变化,血氧含量有所下降.3例移植物单纯用Collins液灌注而不做灌洗者出现高血钾症.血流动力学以无肝前手术开始后20分钟值为对

  11. Effect of various sources and levels of iodine, as well as the kind of diet, on the performance of young laying hens, iodine accumulation in eggs, egg characteristics, and morphotic and biochemical indices in blood. (United States)

    Słupczyńska, M; Jamroz, D; Orda, J; Wiliczkiewicz, A


    Young hens were fed over a period of 150 d with 2 kinds of diets including corn and soybean meal or corn, soybean, and rapeseed meal. Diets were enriched with potassium iodide (KI) or potassium iodate (KIO3) as an I source in amounts equal to 1, 3, or 5 mg of supplemented I/kg of feed. The hen performance, egg quality, hematological and morphotic indices in blood, hepatic enzyme activity, lipid indices in blood serum as well as I accumulation in wet egg content were determined. Introduction of 00-variety rapeseed meal into the diet improved the laying rate and feed conversion (P < 0.05); however, better egg weight was noted by feeding the hens with a diet without rapeseed meal. Use of KI as an I source enhanced the egg weight. The increased I level in the diet had an equivocal influence on egg weight, improved the feed conversion per 1 kg of eggs, and decreased the proportion of damaged eggs. The use of corn, soybean, and rapeseed meal in hen diets significantly improved yolk color; similar results were noted after an increase in I levels in the diets after 3 mo of feeding. Hematological indices of hen blood demonstrated significantly higher red blood cells numbers and hemoglobin concentrations with the use of KI. The use of a diet containing rapeseed meal led to an enhancement of hepatic enzyme activity, especially of alkaline phosphatase (P = 0.007). Lipid metabolism indices were not influenced by the kind of diet or the I source or level. The accumulation of I in wet egg content was negatively influenced by the use of a diet containing rapeseed meal (P = 0.000). The application of KI as an I source enhanced (P = 0.003) the accretion of I in eggs after 5 mo of treatment. Enhanced I supply significantly increased accumulation of I in eggs (P = 0.000) after 3 and 5 mo of the experiment from 260 and 310 to 1,011 and 1,256 µg/kg of wet egg content, respectively.

  12. Biochemical Hypermedia: Galactose Metabolism.

    Directory of Open Access Journals (Sweden)

    J.K. Sugai


    Full Text Available Introduction: Animations of biochemical processes and virtual laboratory environments lead to true molecular simulations. The use of interactive software’s in education can improve cognitive capacity, better learning and, mainly, it makes information acquisition easier. Material and Methods: This work presents the development of a biochemical hypermedia to understanding of the galactose metabolism. It was developed with the help of concept maps, ISIS Draw, ADOBE Photoshop and FLASH MX Program. Results and Discussion: A step by step animation process shows the enzymatic reactions of galactose conversion to glucose-1-phosphate (to glycogen synthesis, glucose-6-phosphate (glycolysis intermediary, UDP-galactose (substrate to mucopolysaccharides synthesis and collagen’s glycosylation. There are navigation guide that allow scrolling the mouse over the names of the components of enzymatic reactions of via the metabolism of galactose. Thus, explanatory text box, chemical structures and animation of the actions of enzymes appear to navigator. Upon completion of the module, the user’s response to the proposed exercise can be checked immediately through text box with interactive content of the answer. Conclusion: This hypermedia was presented for undergraduate students (UFSC who revealed that it was extremely effective in promoting the understanding of the theme.

  13. Haematology and serum biochemical parameters in free-ranging African side neck turtle (Pelusios sinuatus in Ibadan, Nigeria

    Directory of Open Access Journals (Sweden)

    A.O. Omonona


    Full Text Available The haematology and serum biochemical parameters in free-ranging African side neck turtle (Pelusios sinuatus in Ibadan, Nigeria was carried out with the view of establishing baseline blood health indices of this species and generating data which could be useful in the comparative physiology of turtles. A total of sixty free ranging turtles comprising juveniles and adults of both sexes were used for the study. The mean values for the RBC, PCV, Hb, MCV, MCH and WBC counts observed in male juvenile were significantly higher (P < 0.05 than those of the females. Nevertheless, in adult turtles, the mean values for the RBC, PCV, Hb, MCV, MCH and WBC counts observed in females were significantly higher (P < 0.05 than those of the males. Similarly, in juvenile turtles, the absolute heterophil, lymphocyte and monocyte counts in females were relatively higher (P < 0.05 than that of male while in adult turtles there were no significant differences (P < 0.05 in these parameters between the males and females. There were no significant difference (P < 0.05 in the values for the total protein, albumin, globulin, creatinine, serum glutamic-oxalacetic transaminase (SGOT, serum glutamic-pyruvic transaminase (SGPT and blood urea nitrogen in the males and females of both the juvenile and adult turtles. The outcome of this study presents baseline data on the haematology and serum biochemical parameters in free-ranging African side neck turtle (Pelusios sinuatus in Ibadan, Nigeria, which could also serve as a template for the comparative physiology of fresh and sea turtles.

  14. Hematological and serum biochemical parameters of blood in adolescent rats and histomorphological changes in the jejunal epithelium and liver after chronic exposure to cadmium and lead in the case of supplementation with green tea vs black, red or white tea. (United States)

    Tomaszewska, Ewa; Winiarska-Mieczan, Anna; Dobrowolski, Piotr


    Rats were used to check whether regular consumption of black, red, or white tea would have a protective effect similar to the action of green tea on the intestine and liver in the case of exposure to Cd and Pb within the limits of human environmental exposure to these elements. Rats at the age of 6 weeks were divided into the control and four groups supplemented with green (GT), black (BT), red (RT), or white (WT) tea extracts. Their diet (except the control) was mixed with 7 mg Cd/kg and 50mg Pb/kg. The experiment lasted 12 weeks. The effects of administration of tea in Cd- and Pb-poisoned rats on plasma biochemical parameters and the jejunal epithelium and liver were determined. The highest body mass was found in the GT group. The highest hemoglobin and Fe concentrations were in the control and GT groups. The highest activity of AST was in groups poisoned with Cd and Pb independently on supplementation. The highest ALT activity was in BT and RT groups with lower content of polifenoles. Pb and Cd disturbed the liver leading to necrosis and fatty degenerative changes, and a loss of normal architecture of the hepatocytes. Rats from the GT group had the highest cell proliferation rate in intestinal glands and the largest absorptive surface. Black, red, and white tea exerted a varied impact on the histological structure and innervation of the small intestine wall as well as on the absorptive function of small intestine mucosa in rats poisoned with Pb and Cd than green tea. On the other hand, taking into account the number of apoptotic cells, the effect of the teas was the same. Moreover, it is clear that long term exposure to Cd and Pb contamination causes toxic effect in the liver.

  15. Study of Some Hematological and Biochemical Parameters of Rainbow Trout (Oncorhynchus mykiss) Fry


    Salman Rauoof CHALKOO; Imtiyaz Ahmed SHEIKH; Lone, Ghulam Nabi; Shaukath Ali MIR


    This study was done to investigate of some hematological and biochemical factors of rainbow trout (Oncorhynchus mykiss) fry in Kashmir. About 50 pools of blood samples from diseased fry were collected within 30 months from November 2008 till March 2011 from three hatchery farms. In addition 30 pools of blood samples as control group were collected randomly from same farms. Each blood samples were examined for whole blood examination and blood enzymes measurement. It consist of total leukocyte...

  16. Produção leiteira, composição do leite e perfil bioquímico sanguíneo de vacas lactantes sob suplementação com sal aniônico Milk yield, milk composition and biochemical blood profile of lactating cows supplemented with anionic salt

    Directory of Open Access Journals (Sweden)

    Lúcia Treptow Marques


    Full Text Available Objetivou-se avaliar os efeitos da inclusão de sal aniônico na dieta sobre as características físicas e a composição química do leite, o perfil bioquímico sanguíneo, a produção de leite, o peso e a condição corporal de vacas em lactação. Foram utilizadas 20 vacas da raça Jersey distribuídas em duas dietas, uma sem adição de sal aniônico e outra com adição de 0,25 kg/dia de cloreto de amônio. O delineamento empregado foi o completamente casualizado. As vacas sob suplementação com sal aniônico apresentaram menor pH urinário e aumento das concentrações sanguíneas de ureia, cálcio e magnésio. Com a dieta aniônica, o leite precipitou com menores concentrações de etanol, apresentou menor densidade, porcentagens de lactose, proteína e imunoglobulina, porém maior teor de cálcio iônico. O fornecimento de dieta aniônica durante a lactação resultou em acidose metabólica, o que alterou o perfil bioquímico sanguíneo e as características físico-químicas do leite.The objective of this work was to evaluate the inclusion of anionic salt in the diet on the physical characteristics and chemical composition of milk, biochemical blood profile, milk yield, body weight and condition. It was used 20 Jersey cows, distributed into two diets, a diet with no addition of anionic salt and one diet with addition of 0.25 kg/day of ammonium chloride. It was used a completely randomized design. Cows supplemented with anionic salt presented lower urinary pH and increase of blood contents of urea, calcium and magnesium. With the anionic diet, milk precipitated with lower ethanol concentration and it presented lower density, percentages of lactose, protein and immunoglobulin, but higher ionic calcium content. Supply of anionic diet during lactation resulted in metabolic acidosis, which altered the blood biochemical profile and milk physical-chemical characteristics.

  17. Serum biochemical parameters of farmed carp (Cyprinus carpio

    Directory of Open Access Journals (Sweden)

    Tanţi Patriche


    Full Text Available Despite advances in ichthyo-pathology of recent years, interpretation of fish serum biochemical parameters is often difficult by lack of reference values. That is why to know the value of the serum biochemical parameters can be a useful tool for monitoring health status, detecting illnesses and responses to therapy. This paper provides data concerning biochemical composition of carp serum (Cyprinus carpio bred at Brateş Farm of Institute of Research and Development for Aquatic Ecology, Fishing and Aquaculture from Galaţi and Pleaşa Farm from Ploieşti, Romania. In research conducted onCyprinus carpio were determined following serum biochemical parameters: glucose (GLU, total proteins (TP, blood urea nitrogen (BUN, cholesterol (CHOL, triglyceride (TRIG, sodium (Na, potassium (K, calcium (Ca, magnesium (Mg, phosphorus (P, iron (Fe.

  18. Selected clinical, biochemical, and electrolyte alterations in anesthetized captive tigers (Panthera tigris) and lions (Panthera leo). (United States)

    Reilly, Sabrina; Seddighi, M Reza; Steeil, James C; Sura, Patricia; Whittemore, Jacqueline C; Gompf, Rebecca E; Elliott, Sarah B; Ramsay, Edward C


    A prospective study to assess changes in selected plasma biochemistry and electrolyte values, plasma insulin and aldosterone concentrations, and electrocardiography (ECG) was performed on eight female captive tigers (Panthera tigris) and three lions (Panthera leo) undergoing general anesthesia for elective laparoscopic ovariectomy. Each animal was sedated with medetomidine (18-25 microg/kg) and midazolam (0.06-0.1 mg/kg) intramuscularly, and anesthesia was induced with ketamine (1.9-3.5 mg/kg) intramuscularly and maintained with isoflurane. Venous blood samples were collected and analyzed for plasma biochemistry parameters and insulin and aldosterone concentrations. An ECG was recorded at the time of each blood sample collection. Mean plasma potassium, glucose, phosphorus, and aldosterone concentrations increased during anesthesia (P < or = 0.05). One tiger developed hyperkalemia (6.5 mmol/L) 2.5 hr after anesthetic induction. Plasma insulin concentrations were initially below the low end of the domestic cat reference interval (72-583 pmol/L), but mean insulin concentration increased (P < or = 0.05) over time compared with the baseline values. Three tigers and two lions had ECG changes that were representative of myocardial hypoxemia. Based on these results, continuous monitoring of clinical and biochemical alterations during general anesthesia in large nondomestic felids is warranted, and consideration should be given to reversal of medetomidine in these animals should significant changes in electrolytes or ECG occur.

  19. Anatomical and biochemical investigation of primary brain tumours

    Energy Technology Data Exchange (ETDEWEB)

    Del Sole, A. [Univ. di Milano (Italy); Falini, A. [Univ. Vita e Salute (Italy). IRCCS; Ravasi, L.; Ottobrini, L.; Lucignani, G. [Univ. di Milano (Italy). Ist. di Scienze Radiologiche; De Marchis, D. [Univ. di Milano-Bicocca (Italy); Bombardieri, E. [Istituto Nazionale dei Tumori, Milano (Italy)


    Cancerous transformation entails major biochemical changes including modifications of the energy metabolism of the cell, e.g. utilisation of glucose and other substrates, protein synthesis, and expression of receptors and antigens. Tumour growth also leads to heterogeneity in blood flow owing to focal necrosis, angiogenesis and metabolic demands, as well as disruption of transport mechanisms of substrates across cell membranes and other physiological boundaries such as the blood-brain barrier. All these biochemical, histological and anatomical changes can be assessed with emission tomography, X-ray computed tomography (CT), magnetic resonance imaging (MRI) and magnetic resonance spectroscopy (MRS). Whereas anatomical imaging is aimed at the diagnosis of brain tumours, biochemical imaging is better suited for tissue characterisation. The identification of a tumoural mass and the assessment of its size and vascularisation are best achieved with X-ray CT and MRI, while biochemical imaging can provide additional information that is crucial for tumour classification, differential diagnosis and follow-up. As the assessment of variables such as water content, appearance of cystic lesions and location of the tumour are largely irrelevant for tissue characterisation, a number of probes have been employed for the assessment of the biochemical features of tumours. Since biochemical changes may be related to the growth rate of cancer cells, they can be thought of as markers of tumour cell proliferation. Biochemical imaging with radionuclides of processes that occur at a cellular level provides information that complements findings obtained by anatomical imaging aimed at depicting structural, vascular and histological changes. This review focusses on the clinical application of anatomical brain imaging and biochemical assessment with positron emission tomography, single-photon emission tomography and MRS in the diagnosis of primary brain tumours, as well as in follow-up. (orig.)

  20. TAPIR--Finnish national geochemical baseline database. (United States)

    Jarva, Jaana; Tarvainen, Timo; Reinikainen, Jussi; Eklund, Mikael


    In Finland, a Government Decree on the Assessment of Soil Contamination and Remediation Needs has generated a need for reliable and readily accessible data on geochemical baseline concentrations in Finnish soils. According to the Decree, baseline concentrations, referring both to the natural geological background concentrations and the diffuse anthropogenic input of substances, shall be taken into account in the soil contamination assessment process. This baseline information is provided in a national geochemical baseline database, TAPIR, that is publicly available via the Internet. Geochemical provinces with elevated baseline concentrations were delineated to provide regional geochemical baseline values. The nationwide geochemical datasets were used to divide Finland into geochemical provinces. Several metals (Co, Cr, Cu, Ni, V, and Zn) showed anomalous concentrations in seven regions that were defined as metal provinces. Arsenic did not follow a similar distribution to any other elements, and four arsenic provinces were separately determined. Nationwide geochemical datasets were not available for some other important elements such as Cd and Pb. Although these elements are included in the TAPIR system, their distribution does not necessarily follow the ones pre-defined for metal and arsenic provinces. Regional geochemical baseline values, presented as upper limit of geochemical variation within the region, can be used as trigger values to assess potential soil contamination. Baseline values have also been used to determine upper and lower guideline values that must be taken into account as a tool in basic risk assessment. If regional geochemical baseline values are available, the national guideline values prescribed in the Decree based on ecological risks can be modified accordingly. The national geochemical baseline database provides scientifically sound, easily accessible and generally accepted information on the baseline values, and it can be used in various

  1. Effect of dietary cation-anion balance on lactating performance and blood biochemical parameters of perinatal dairy cows%不同阴阳离子水平日粮对围产期奶牛泌乳性能和血液生化指标的影响

    Institute of Scientific and Technical Information of China (English)

    郭冬生; 彭小兰; 龚群辉


    In order to study the effect of dietary cation-anion balance (DCAB) on lactating performance and blood biochemical parameters in perinatal dairy cows, twenty Holstein lactating cows were divided into 4 experimental groups ran- domly , with 5 replicates per group, and the cows were fed with the diets containing four levels of DCAB, 50.80 mmol/kg, -46.91 mmol/kg, -128.45 mmol/kg, and -213.44 mmol/kg respectively. The daily milk yield, percentages of milk fat and protein, feed intake, the urinary pH value, the ionic concentration of serum and blood biochemical parameters were determined. The result showed that the milk production of experimental group Ⅱ ( -128.45mmol/kg) was increased by 13.4% (P<0.05), the blood serum Ca2+ concentration was increased by 12.9% (P<0. 05), the blood serum hydroxyproline ( HYP) concentration was increased by 12. 0% (P<0. 01) ,the blood serum alkaline phos-phatase (ALP) concentration was increased by 26. 9% (P<0.01) ,the blood serum bone gla protein (BGP) concentration was increased by 9.2% (P<0. 05), the blood serum parathyroid gland (PTH) concentration was decreased by 20. 54% (P< 0. 01) ,and the blood serum calcitonin (CT) concentration was increased by 27. 72% (P<0.01), compared with the control group (50.80 mmol/kg). The experiment demonstrated that when prepartum diets were supplemented with anionic salts, the feed intake and the urinary pH value of cows decreased, the milk production, the blood serum Ca2+ concentration and the blood serum CT concentration increased, the blood serum HYP, ALP and BGP concentration elevated, and the blood serum craetinephosphokinase (CK) and alanine transaminase (ALT) concentration droped.%为了研究阴离子盐对围产期奶牛产奶性能和血液生化指标的影响,选取20头中国荷斯坦奶牛,随机分成4组,每组5头,分别饲喂日粮阴阳离子平衡(DCAB)值为50.80mmol/kg、-46.91mmol/kg、-128.45mmol/kg、-213.44mmol/kg的日粮,测定动物采食量、尿液pH值、产奶量

  2. Effects of adding aqueous extract of Tribulus terrestris to diet on productive performance, egg quality characteristics, and blood biochemical parameters of laying hens reared under low ambient temperature (6.8 ± 3 °C) (United States)

    Akbari, Mohsen; Torki, Mehran


    A study was conducted using 144 laying hens to evaluate the effects of adding aqueous extract of Tribulus terrestris to diets on productive performance, egg quality traits, and some blood parameters of laying hens reared under cold stress condition (6.8 ± 3 °C). The birds were randomly assigned to each of four dietary treatments (C, T1, T2, and T3) with six replicate cages of six birds. Diet inclusion of aqueous extract of T. terrestris at the rate of 10, 20, and 30 ml/Lit offered to groups T1, T2, and T3, respectively, while group C served as the control diet with no addition. Feed intake (FI), feed conversion ratio (FCR), egg weight (EW), egg production (EP), and egg mass (EM) were evaluated during the 42-day trial period. The EP and EM increased, whereas FCR decreased ( P < 0.001) in the hens fed the extract-included diet as compared to those fed the basal diet. The serum content of cholesterol decreased and the thickness of egg shell increased in the hens fed the T2 and T3 diet compared to those fed the basal diet. Overall from the results of the present experiment, it can be concluded that diet supplementation with aqueous extract of T. terrestris has beneficial effects on productive performance of laying hens reared under cold stress condition.

  3. Effects of adding aqueous extract of Tribulus terrestris to diet on productive performance, egg quality characteristics, and blood biochemical parameters of laying hens reared under low ambient temperature (6.8 ± 3 °C). (United States)

    Akbari, Mohsen; Torki, Mehran


    A study was conducted using 144 laying hens to evaluate the effects of adding aqueous extract of Tribulus terrestris to diets on productive performance, egg quality traits, and some blood parameters of laying hens reared under cold stress condition (6.8 ± 3 °C). The birds were randomly assigned to each of four dietary treatments (C, T1, T2, and T3) with six replicate cages of six birds. Diet inclusion of aqueous extract of T. terrestris at the rate of 10, 20, and 30 ml/Lit offered to groups T1, T2, and T3, respectively, while group C served as the control diet with no addition. Feed intake (FI), feed conversion ratio (FCR), egg weight (EW), egg production (EP), and egg mass (EM) were evaluated during the 42-day trial period. The EP and EM increased, whereas FCR decreased (P terrestris has beneficial effects on productive performance of laying hens reared under cold stress condition.


    Directory of Open Access Journals (Sweden)



    Full Text Available Diabetic nephropathy is a clinical syndrome characterized by the following- Persistent albuminuria (>300mg/d or >200μg/min, that is confirmed on at least 2 occasions 3-6 months apart diabetic, progressive decline in the Glomerular Filtration Rate (GFR, elevated arterial blood pressure. The earliest biochemical criteria for the diagnosis of diabetic nephropathy is the presence of micro-albumin in the urine, which if left untreated will eventually lead to End-Stage Renal Disease (ESRD. Micro-albuminuria refers to the excretion of albumin in the urine at a rate that exceeds normal limits. The current study was conducted to establish the prevalence of micro-albuminuria in a sequential sample of diabetic patients attending hospital and OPD Clinic to determine its relationship with known and putative risk factors to identify micro- and normo-albuminuric patients in their sample for subsequent comparison in different age, sex, weight and creatinine clearance of the micro- and normo-albuminuric patients. This cross-sectional analytical study was conducted in one hundred patients at Saraswathi Institute of Medical Sciences, Anwarpur, Hapur, U. P. Patients having diabetes mellitus in different age group ranging from 30 to 70 years were selected. Data was analysed by SPSS software. Micro-albuminuria was observed in 35% in patients with type 2 diabetes mellitus. It was observed that 65% patients were free from any type of albuminuria. Also micro-albuminuria was present in 10% of the patients less than 50 yrs. of age, while 15% of the patients more than 50 yrs. of age were having micro-albuminuria. There was a statistically significant correlation of micro-albuminuria with duration of diabetes. Incidence of micro-albuminuria increases with age as well as increased duration of diabetes mellitus. Our study shows that only 5% patients developed macro-albuminuria. Glycosylated haemoglobin and fasting plasma glucose was significantly raised among all these

  5. Characterization of changes in gene expression and biochemical pathways at low levels of benzene exposure

    NARCIS (Netherlands)

    Thomas, Reuben; Hubbard, Alan E.; McHale, Cliona M.; Zhang, Luoping; Rappaport, Stephen M.; Lan, Qing; Rothman, Nathaniel; Vermeulen, Roel; Guyton, Kathryn Z.; Jinot, Jennifer; Sonawane, Babasaheb R.; Smith, Martyn T.


    Benzene, a ubiquitous environmental pollutant, causes acute myeloid leukemia (AML). Recently, through transcriptome profiling of peripheral blood mononuclear cells (PBMC), we reported dose-dependent effects of benzene exposure on gene expression and biochemical pathways in 83 workers exposed across

  6. Changes in blood pressure indices in normotensive adults after the consumption of lemongrass tea

    Institute of Scientific and Technical Information of China (English)

    Christopher Ekpenyong; Eme Osim


    Objective:To accurately evaluate the effect of lemongrasses tea (LGT) on blood pressure (BP) indices in normal humans. Methods:A total of 105 participants were sub-divided into 3 groups with 35 in each group and they were administered with LGT prepared from 2, 4 or 8 g of the lemongrass leaf powder (LP) for 30 days, respectively. They were evaluated for various BP indices and other clinical and biochemical parameters at days 0, 10 and 30 after the administration of LGT using standard methods. Results:At day 10, systolic blood pressure and diastolic blood pressure (DBP) were lower than baseline levels. The mean arterial pressure was slightly reduced, while pulse pressure and heart rate (HR) significantly increased in subjects administered with LGT prepared from 4 or 8 g of the LP. At day 30, systolic blood pressure and DBP remained decreased in participants administered with LGT prepared from 4 g of the LP. DBP normalized in participants administered with LGT prepared from 4 g of the LP. The mean arterial pressure and HR decreased further in participants administered with LGT prepared from 8 g of the LP, but HR normalized in subjects treated with LGT prepared from 4 g of the LP. Pulse pressure almost returned to baseline level. Conclusions:Ingestion of LGT may be associated with decreased BP indices in normotensive humans due to its varied bioactive constituents and their activities.

  7. Changes in blood pressure indices in normotensive adults after the consumption of lemongrass tea

    Directory of Open Access Journals (Sweden)

    Christopher Ekpenyong


    Full Text Available Objective: To accurately evaluate the effect of lemongrasses tea (LGT on blood pressure (BP indices in normal humans. Methods: A total of 105 participants were sub-divided into 3 groups with 35 in each group and they were administered with LGT prepared from 2, 4 or 8 g of the lemongrass leaf powder (LP for 30 days, respectively. They were evaluated for various BP indices and other clinical and biochemical parameters at days 0, 10 and 30 after the administration of LGT using standard methods. Results: At day 10, systolic blood pressure and diastolic blood pressure (DBP were lower than baseline levels. The mean arterial pressure was slightly reduced, while pulse pressure and heart rate (HR significantly increased in subjects administered with LGT prepared from 4 or 8 g of the LP. At day 30, systolic blood pressure and DBP remained decreased in participants administered with LGT prepared from 4 g of the LP. DBP normalized in participants administered with LGT prepared from 4 g of the LP. The mean arterial pressure and HR decreased further in participants administered with LGT prepared from 8 g of the LP, but HR normalized in subjects treated with LGT prepared from 4 g of the LP. Pulse pressure almost returned to baseline level. Conclusions: Ingestion of LGT may be associated with decreased BP indices in normotensive humans due to its varied bioactive constituents and their activities.

  8. Effect of Milk Replacer on Upgrowth Performance and Blood Biochemical Indices in Sika Deer Lamps%代乳料对梅花鹿仔鹿生长发育及血液生化指标的影响

    Institute of Scientific and Technical Information of China (English)

    王凯英; 钟伟; 李光玉; 刘佰阳; 李丹丽; 苏伟林; 高秀华


    为研究代乳料对仔鹿的影响,选择出生日期相近的梅花鹿仔鹿28只,随机分成A、B、C 3组,分别用母乳、牛奶、代乳料进行60d的饲养试验,试验结束时采血检测血液生化指标.结果表明:不同处理对仔鹿平均日增重影响显著(P<0.05),呈阶段性变化,24日龄前A组最高、24~64日龄C组最高;A组仔鹿胸围平均日增长显著高于B、C组(P<0.05);各组间仔鹿肩高、体长无显著差异;不同处理间血清总蛋白(AT)浓度差异显著(P<0.05),血清免疫球蛋白(IgG)浓度差异极显著(P<0.01),均以A组最高;不同处理间血清白蛋白(ALB)、尿素氮(BUN)、葡萄糖(GLU)浓度和血清碱性磷酸酶(AKP)活性差异不显著(P>0.05).%To study the effects of milk replacer on the upgrowth performance and blood biochemistry indices in deer lamps, an experiment was conducted using 28 four day-old dEer lamps respectively fed on doe's milk, milk and milk replacer.A single factor variance analysis experiment was designed.The deer lamps were randomly divided into three groups, A, B and C (A has 8, B has 10 and C has 10) based on body weight and birth similarity.Group A got along with doe and fed on doe's milk, group B on milk,and group C on milk replacer.The results showed:the effects of milk replacer on mortality rate were significant, A being 12.5%, B 30.8%, C 22.2%; the effects of milk replacer on average daily gain (ADG) were significant( P < O.05)and varied by phase, A being the best before 24 day-old, C the best from 24 to 64 day-old.The chest circumference of A was significant hyper-B and C( P < 0.05), shoulder height and body length had no significant difference.Total protein(TP) of A was evidently higher than the other two groups( P < 0.05), IgG of A was the highest among all groups( P < 0.01 ), milk replacer did not affect level of albumin (ALB), blood urea nitrogen (BUN) or glucose (GLU) in serum of deer lambs (P > 0.05), and the alkaline

  9. Use of predefined biochemical admission profiles does not reduce the number of tests or total cost

    DEFF Research Database (Denmark)

    Pareek, Manan; Haidl, Felix; Folkestad, Lars


    The objective of this pilot study was to evaluate whether the use of predefined biochemical profiles as an alternative to individually ordered blood tests by the treating physicians resulted in fewer tests or a lower total cost.......The objective of this pilot study was to evaluate whether the use of predefined biochemical profiles as an alternative to individually ordered blood tests by the treating physicians resulted in fewer tests or a lower total cost....

  10. Baseline hematologic, endocrine, and clinical chemistry values in ducks and roosters. (United States)

    Spano, J S; Pedersoli, W M; Kemppainen, R J; Krista, L M; Young, D W


    Venous blood samples were collected at 3-day intervals for a total of six samples from each of five adult male pekin ducks and five adult Ross roosters. Twenty biochemical, six hematologic, and three endocrine determinations were performed on each blood or serum sample collected. The data obtained provide reference values for future studies of avian species and illustrate the utility of an automated clinical chemistry analyzer in assessing multiple serum biochemistry values in small sample volumes obtained from birds.

  11. Life Support Baseline Values and Assumptions Document (United States)

    Anderson, Molly S.; Ewert, Michael K.; Keener, John F.; Wagner, Sandra A.


    The Baseline Values and Assumptions Document (BVAD) provides analysts, modelers, and other life support researchers with a common set of values and assumptions which can be used as a baseline in their studies. This baseline, in turn, provides a common point of origin from which many studies in the community may depart, making research results easier to compare and providing researchers with reasonable values to assume for areas outside their experience. With the ability to accurately compare different technologies' performance for the same function, managers will be able to make better decisions regarding technology development.

  12. Determination of the hemocytology and blood biochemical parameters in imported malarial patients in Beijing areas%北京地区输入性疟疾血细胞及生化检验指标变化的敏感性分析

    Institute of Scientific and Technical Information of China (English)

    邹洋; 李晶晶; 黄敏君; 谷俊朝; 魏巍; 李小丽; 王磊; 王非; 李威; 齐志群; 田小军; 安亦君


    Objective To investigate the clinical significance by determining the biochemical indicators on urine and blood for early diagnosis of imported malarial patients. Methods The parameters of urine and blood biochemistry were retrospectively analyzed in 55 clinically confirmed patients with imported malaria in Beijing area with multiple Logistic regression by using software SPSS 18.0, and the results were compared with health controls. Results The biochemical parameters, including decreased blood platelet(OR=0.980, P=0.003), elevated glutamic-oxaloacetic transaminase(OR=0.969, P=0.013) and blood urea nitrogen(OR=1.907, P=0.013), were statistically significant in early stage of infection in imported malarial patients. Conclusion Bio⁃chemical parameters changed significantly in the early stage of infection in patients of imported malaria, suggest⁃ing that determination of these indicators can lead to early diagnosis and treatment of this disease.%目的:分析输入性疟疾患者确诊前的各项血、尿常规和血生化等检查指标,探讨各检测参数指标的临床早期诊断方面的意义。方法使用SPSS 18.0软件,回顾性分析55例临床确诊输入性疟疾患者的各项血、尿常规和血生化等检查指标,并建立正常对照组。采用Logistic多元回归分析,初步完成输入性疟疾各检验参数指标的分析。结果血常规中血小板减少(OR=0.980,P=0.003),肝功能中谷草转氨酶升高(OR=0.969,P=0.013),和尿素氮的变化(OR=1.907,P=0.013),在疟疾早期各生化指标变化中具有明显意义。结论通过本次研究,初步表明血常规中血小板减少,肝功能中谷草转氨酶升高,和尿素氮的变化,在疟疾早期各生化指标变化中具有明显意义。其综合判断,有利于输入性疟疾诊断早期预警,降低误诊率,提高诊断率。对疟疾的临床早期诊断具有重要意义。

  13. 不同方案消除血脂对生化测定干扰的研究%Study of different schemes for eliminating influence of blood lipid on biochemical detection

    Institute of Scientific and Technical Information of China (English)

    甄广怀; 周玉萍; 陈达富


    Objective To investigate the effects of high‐speed centrifugation ,dilution and ether extraction methods in eliminating the blood lipid interference to provide reference for eliminating this interference .Methods 100 cases of lipid samples ,the specimens were divided into 4 parts ,1 part of each group ,three groups were given high‐speed centrifugation ,dilution and ether extraction methods ,and another group was detected as the original plas‐ma .The differences before and after processing were compared among groups .The high‐speed centrifugation ,dilution and ether extraction methods adopted the variance analysis .Results Ca2+ ,UA ,Cr ,GLU ,ALB ,TP ,DBIL ,TBIL , AMY ,CK‐MB ,CK ,HBDH ,LDH ,γGT ,AST and ALT values detected by 3 kinds of method had statistically signifi‐cant differences between before and after processing (P<0 .05) .The detection value in descending order of serum dope testing ,deionized water dilution ,low‐speed centrifugation ,ether extraction .Conclusion The ether extraction method significantly affects the protein detection items ,the influence of ionized water dilution is minimum ,three kinds of method can be combined for use in clinical work .%目的:探讨高速离心法、稀释法、乙醚萃取法消除血脂干扰的效果,为消除血脂对生化检测的干扰提供参考。方法收集100例血脂标本,将标本分为4份,每组25份,其中3组分别给予高速离心法、稀释法、乙醚萃取法处理,另1组原血浆检测。比较处理前后各组间的差异,并对高速离心法、稀释法、乙醚萃取法组采用方差分析。结果3种方法对Ca2+、尿酸(UA)、肌酐(Cr)、血糖(GLU)、清蛋白(ALB)、总蛋白(TP)、直接胆红素(DBIL)、总胆红素(TBIL)、淀粉酶(AMY)、肌酸激酶同工酶(CK‐MB)、肌酸激酶(CK)、羟丁酸脱氢酶(HBDH)、乳酸脱氢酶(LDH)、谷氨酰基转移酶(γGT)、天门冬氨酸氨基转

  14. Effect of complete colostrum milking on blood biochemical parameters in perinatal dairy cow%一次性挤净初乳对围产期奶牛血液生化指标的影响

    Institute of Scientific and Technical Information of China (English)

    王小虎; 张学峰; 董晓庆; 王光辉; 甄玉国


    试验旨在能够有效地控制围产期奶牛血钙水平的前提下,验证奶牛在产后可进行一次性挤净初乳。选择体重、胎次相近,机能状态、生产性能良好,且即将入围产期的中国黑白花奶牛16头,按照随机分配原则分为4组,即低钙不净奶组、低钙净奶组、阴离子盐净奶组、阴离子盐不净奶组,每组4头。分别饲喂低钙日粮或阴离子盐日粮,低钙日粮组的日粮干物质含钙水平为0.36%,阴离子盐日粮组的DCAD为-68.51 mEq/kg DM。试验期为产前21 d到产后60 d。结果表明,产前低钙日粮和产前阴离子盐日粮均可有效控制围产期血钙水平,两种营养调控措施差异不显著(P>0.05);产后一次性挤净初乳对奶牛产后钙水平影响不显著(P>0.05)。结果提示,预防围产期奶牛产后瘫痪的途径有两条:即产前饲喂低钙日粮或阴离子盐日粮,并且产后一次性挤净初乳对奶牛产后钙水平无影响。%The premise of the test is to effectively control the perinatal cows' serum calcium level, and to verify whether the cows can be one-time squeezed of net colostrum postpartum. The experiment in-volved 16 high performing Chinese Holstein dairy cows with similar body weight, parity and functional status. There were four treatment groups (low calcium not net milked, low calcium net milked, anionic salts net milked and anionic salts not net milked) with four animals per group in a randomized design. The animals were fed with either low calcium or anionic salts diet. In the low calcium diet group, the dietary dry matter calcium level was 0.36%, anionic salt diet group DCAD was-68.51 mEq/kg DM. The trial period for prenatal 21 d to 60 d postpartum. The results show that, the prenatal low calcium dietary anionic salts and prenatal diet can effectively control the perinatal blood calcium level , two kinds of nutritional regulation measures had not significant difference(P>0

  15. 2株H5N1亚型禽流感病毒人工感染鸭的11项血液生化指标测定%Eleven Blood Biochemical Indexes Assay of Ducks Experimentally Infected with Two Avian Influenza Viruses(H5N1)

    Institute of Scientific and Technical Information of China (English)

    叶远兰; 周泉鹤; 李玉谷


    鸭肌肉接种禽流感病毒A/duck/Guangdong/185/2004(H5N1)和A/duck/Guangdong/221/2004(H5N1)后,血清中的丙氨酸氨基转移酶、天门冬氨酸氨基转移酶、碱性磷酸酶、总蛋白、肌酐、总胆固醇、钙和磷明显升高;葡萄糖和甘油三酯明显降低;白蛋白表现为先下降后升高的变化趋势.作者认为,检测这些血液生化指标对于诊断禽流感具有一定的参考价值.%Ducks intramuscularly inoculated with avian influenza virus strains, A/duck/Guangdong/185/2004 (H5N1) and A/duck/Guangdong/221/2004(H5N1), showed that the serum alanine aminotransferase(glutamic-pyruvic transminase), aspartate aminotransferase(glutamic-oxaloacetic transminase), alkaline phosphatase, total protein, creatinine, cholesterol, calcium and phosphorus were significantly increased; while glucose and triglycerides were obviously decreased; albumin was decreased at first and increased later. We considered that assaying these blood biochemical indexes were useful for diagnosing avian influenza.

  16. NRAO Very Long Baseline Array (VLBA) (United States)

    Federal Laboratory Consortium — The Very Long Baseline Array (VLBA) comprises ten radio telescopes spanning 5,351 miles. It's the world's largest, sharpest, dedicated telescope array. With an eye...

  17. SSA FITARA Common Baseline Implementation Plan (United States)

    Social Security Administration — This document describes the agency's plan to implement the Federal Information Technology Acquisition Reform Act (FITARA) Common Baseline per OMB memorandum M-15-14.

  18. Breton Island, Louisiana Baseline (Geographic, NAD83) (United States)

    U.S. Geological Survey, Department of the Interior — Breton Island, Louisiana Baseline (Geographic, NAD83) consists of vector line data that were input into the Digital Shoreline Analysis System (DSAS) version 4.0,...

  19. Ouroboros - Playing A Biochemical

    Directory of Open Access Journals (Sweden)

    D. T. Rodrigues


    Full Text Available Ouroboros: Playing A Biochemical RODRIGUES,D.T.1,2;GAYER, M.C.1,2; ESCOTO, D.F.1; DENARDIN, E.L.G.2, ROEHRS, R.1,2 1Interdisciplinary Research Group on Teaching Practice, Graduate Program in Biochemistry, Unipampa, RS, Brazil 2Laboratory of Physicochemical Studies and Natural Products, Post Graduate Program in Biochemistry, Unipampa, RS, Brazil Introduction: Currently, teachers seek different alternatives to enhance the teaching-learning process. Innovative teaching methodologies are increasingly common tools in educational routine. The use of games, electronic or conventional, is an effective tool to assist in learning and also to raise the social interaction between students. Objective: In this sense our work aims to evaluate the card game and "Ouroboros" board as a teaching and learning tool in biochemistry for a graduating class in Natural Sciences. Materials and methods: The class gathered 22 students of BSc in Natural Sciences. Each letter contained a question across the board that was drawn to a group to answer within the allotted time. The questions related concepts of metabolism, organic and inorganic chemical reactions, bioenergetics, etc.. Before the game application, students underwent a pre-test with four issues involving the content that was being developed. Soon after, the game was applied. Then again questions were asked. Data analysis was performed from the ratio of the number of correct pre-test and post-test answers. Results and discussion: In the pre-test 18.1% of the students knew all issues, 18.1% got 3 correct answers, 40.9% answered only 2 questions correctly and 22.7% did not hit any. In post-test 45.4% answered all the questions right, 31.8% got 3 questions and 22.7% got 2 correct answers. The results show a significant improvement of the students about the field of content taught through the game. Conclusion: Generally, traditional approaches of chemistry and biochemistry are abstract and complex. Thus, through games

  20. Enzyme and biochemical producing fungi

    DEFF Research Database (Denmark)

    Lübeck, Peter Stephensen; Lübeck, Mette; Nilsson, Lena


    factories for sustainable production of important molecules. For developing fungi into efficient cell factories, the project includes identification of important factors that control the flux through the pathways using metabolic flux analysis and metabolic engineering of biochemical pathways....

  1. Reference values for chinchilla (Chinchilla laniger blood cells and serum biochemical parameters Valores de referência para os parâmetros das células e bioquímica sangüínea da chinchila (Chinchilla laniger

    Directory of Open Access Journals (Sweden)

    Tális de Oliveira Silva


    Full Text Available Raising chinchilla (Chinchilla laniger for commercial purpose has increased significantly; however, hematological and serum biochemical reference values have not yet been determined for chinchillas raised in south Brazil. Establishing blood cells and serum biochemistry reference values might be helpful to evaluate health status of chinchillas and might be used as a tool by clinicians. The purpose of this study was to determine the reference values for blood cells and serum biochemistry of Chinchilla laniger. Blood samples were collected by cardiac puncture from 16 adult males, at the time they were killed to remove the fur coat, and from 8 adult males anesthetized with ketamine and xylazine. Blood cell counts and serum biochemistry analysis were performed using standard techniques and the results were expressed as mean ± SEM. Analysis of blood parameters from post-mortem cardiac punctured and from anesthetized chinchillas indicated that blood samples from anesthetized chinchillas had higher PCV, Hemoglobin, MCHC and WBC (P A criação de chinchila (Chinchilla laniger com objetivos comerciais tem crescido muito nos últimos anos. No entanto, os valores de referência para os parâmetros hematológicos e bioquímicos não foram ainda determinados para chinchilas criados no sul do Brasil. O estabelecimento dos valores de referência para esses parâmetros pode servir de auxílio para a avaliação da saúde das chinchilas e servir de auxílio diagnóstico para o clínico. Esse estudo teve como objetivo determinar os valores de referência das células e da bioquímica sangüínea da Chinchilla laniger. As amostras de sangue foram coletadas por meio de punção cardíaca de 16 machos adultos no momento em que os animais foram mortos para remoção da pele, e de 8 machos adultos após anestesia com xylazina e ketamina. A contagem das células sangüíneas e a análise dos parâmetros bioquímicos foram feitas utilizando-se métodos padronizados em

  2. Mathematical Models of Biochemical Oscillations


    Conrad, Emery David


    The goal of this paper is to explain the mathematics involved in modeling biochemical oscillations. We first discuss several important biochemical concepts fundamental to the construction of descriptive mathematical models. We review the basic theory of differential equations and stability analysis as it relates to two-variable models exhibiting oscillatory behavior. The importance of the Hopf Bifurcation will be discussed in detail for the central role it plays in limit cycle behavior and...

  3. Evaluación del entrenamiento tradicional del caballo criollo chileno de rodeo mediante el análisis de variables fisiológicas y bioquímicas sanguíneas Evaluation of a traditional program of training in chilean rodeo criollo horses through the analysis of physiological and blood biochemical changes

    Directory of Open Access Journals (Sweden)



    used to measure packed cell volume, haemoglobin, total proteins, glucose, triglycerides, lactic acid, insulin, cortisol, and electrolites (Na, Cl and Ca. The activity of enzymes, creatin kinase, lactic dehydrogenase and aspartate amino transferase were obtained before and after each training exercise. The physiological and blood biochemical variables were measured on days 0, 15, 30 and 45 of training. According to the results obtained it is concluded that the traditional on farm training exercise for Chilean Criollo horses is a short term and low speed exercise which represent a physical work of moderate intensity, producing small changes on physiological and blood biochemical variables suggesting that the principal aim of the conditioning program is to improve the ability of horses to stop the steers in the rodeo track than to obtain better physical fitness

  4. Blood biochemical parameters, nitrogen balance and gross energy metabolizability in pigs fed with different dietary electrolyte balancesParâmetros bioquímicos sanguíneos, balanço de nitrogênio e metabolizabilidade da energia bruta em suínos alimentados com dietas contendo diferentes balanços eletrolíticos

    Directory of Open Access Journals (Sweden)

    Luís Daniel Giusti Bruno


    Full Text Available The aim of this study was to evaluate different dietary electrolyte balance (DEB on the blood biochemical parameters, nitrogen balances and crude metabolizable energy of swine in the initial phase. Sixteen barrows were used, averaging 27.95 kg of initial weight, allotted individually in metabolic cages in a randomized blocks design with four treatments (DEB of 160, 208, 257 and 305 mEq/kg and 4 replicates. The blood biochemical parameters analyzed were chloride, potassium, sodium and urea in blood. Urine pH was also evaluated, and the parameters evaluated in nitrogen (N balance were N intake, fecal N, N excreted in urine, absorbed N, N retention, retained N/absorbed N and total excretion of N. The gross energy digestibility and metabolizibility coefficients were determined, and the respectives values of digestible and metabolizable energy. The values of urinary pH increased (P 0.05 between the evaluated DEB. A linear increase (P Objetivou-se com esse trabalho avaliar diferentes balanços eletrolíticos das dietas (BED sobre os parâmetros bioquímicos sanguíneos, balanços de nitrogênio e a metabolizabilidade da energia bruta, de suínos na fase inicial. Foram utilizados 16 suínos, machos castrados, com peso inicial de 27,95 kg, distribuídos individualmente em gaiolas metabólicas, em um delineamento experimental em blocos ao acaso com quatro tratamentos (BED de 160, 208, 257 e 305 mEq/kg e quatro repetições. Os parâmetros bioquímicos sanguíneos avaliados foram cloretos, potássio, sódio e uréia no soro. O pH da urina também foi avaliado, e as variáveis utilizadas no balanço de nitrogênio (N foram o N ingerido, N excretado nas fezes, N excretado na urina, N absorvido, N retido, N retido/N absorvido e excreção total de N. Foram determinados os coeficientes de digestibilidade e metabolizabilidade da energia bruta e seus respectivos valores de energia digestível e metabolizável. Os valores de pH urinário aumentaram (P 0,05 entre

  5. Blood smear (United States)

    ... some red blood cells shaped like spheres ( hereditary spherocytosis ) Increased breakdown of RBCs Presence of RBCs with ... normal Red blood cells, elliptocytosis Red blood cells, spherocytosis Acute lymphocytic leukemia - photomicrograph Red blood cells, multiple ...

  6. Blood culture (United States)

    Culture - blood ... A blood sample is needed . The site where blood will be drawn is first cleaned with an antiseptic such ... organism from the skin getting into (contaminating) the blood sample and causing a false-positive result (see ...

  7. Blood Thinners (United States)

    If you have some kinds of heart or blood vessel disease, or if you have poor blood flow to your brain, your doctor may recommend that you take a blood thinner. Blood thinners reduce the risk of heart ...

  8. Blood transfusions (United States)

    ... this page: // Blood transfusions To use the sharing features on this ... several sources of blood which are described below. Blood From the Public (Volunteer Blood Donation) The most ...

  9. Baseline Omega-3 Index Correlates with Aggressive and Attention Deficit Disorder Behaviours in Adult Prisoners


    Meyer, Barbara J.; Mitchell K Byrne; Carole Collier; Natalie Parletta; Donna Crawford; Winberg, Pia C.; David Webster; Karen Chapman; Gayle Thomas; Jean Dally; Marijka Batterham; Ian Farquhar; Anne-Marie Martin; Luke Grant


    Background There is emerging evidence that the supplementation of omega-3 contributes to a decrease in aggressive behaviour in prison populations. A challenge of such research is achieving statistical power against effect sizes which may be affected by the baseline omega-3 index. There are no published data on the blood omega-3 index with studies of this kind to assess the variability of the blood omega-3 index in conjunction with aggression and attention deficit assessments. Objective To det...

  10. 复合酵母培养物对奶牛产奶性能、氮排放及血液生化指标的影响%Milk production,nitrogen excretion and blood biochemical parameter responses to di-etary addition of compound yeast cultures in dairy cows

    Institute of Scientific and Technical Information of China (English)

    王玲; 吕永艳; 程志伟; 杜高唐; 李金林; 付石军; 孙国强


    为研究饲料中添加复合酵母培养物对奶牛产奶性能、氮排放及血液生化指标的影响,选取年龄、体重、产奶量及泌乳期相近(135±15)d 的荷斯坦奶牛24头,随机分为4组,每个处理6个重复,对照组和3个试验组的复合酵母培养物添加量分别为精料浓度的0,0.8%,1.0%,1.2%,随精料饲喂,测定产奶量、乳成分、氮排放及血液生化指标,结果表明,1)试验2组日均产奶量显著高于对照组(P <0.05),各试验组分别比对照组提高8.48%,10.05%,8.97%。2)复合酵母培养物能显著提高乳脂和乳蛋白率(P <0.05),显著降低牛奶体细胞数(P <0.05),以试验2组最低。3)在氮排放量上,试验2组显著低于对照组(P <0.05),各试验组比对照组分别降低8.47%,12.01%,9.36%。4)在血液生化指标方面,复合酵母培养物能提高血清中总蛋白、球蛋白、血糖、胰岛素水平(P <0.05),降低尿素氮水平(P <0.05)。由此可见,本试验条件下,综合考虑产奶量、乳成分、氮排放及血液生化指标,复合酵母培养物的最佳添加量为精料浓度的1.0%。%This experiment was conducted to determine the effects of compound yeast cultures on the milk pro-duction,nitrogen excretion and blood biochemical parameters of dairy cows.A total of 24 Holstein lactating cows with similar parity,weight,milk yield and lactation stage (135±15 day in milk)were equally assigned to four groups,including one control group and 3 treatment groups,with dietary supplementation of compound yeast cultures at concentrations of 0,0.8%,1.0% and 1.2%,respectively.Milk yield,milk composition,ni-trogen excretion and blood biochemical parameters of each cow were recorded.The results showed:1 )The cows in the respective treatment groups had 8.48%,10.05%,8.97% higher daily milk yield than cows in the control

  11. Association of carotid artery ultrasound traits and blood biochemical indexes with metabolic syndrome in children%儿童代谢综合征与颈动脉超声参数和血液生化指标的相关性研究

    Institute of Scientific and Technical Information of China (English)

    徐梅; 周贵明; 李亚璞; 陈冬玥


    目的:探讨儿童代谢综合征(MS)与颈动脉超声参数和血液生化指标的相关性。方法将30例儿童代谢综合征患者作为MS组,30例儿童单纯性肥胖者作为肥胖非MS组,随机选择30例健康体检儿童作为正常对照组,测量所有入选儿童身高、体重、腰围、臀围、收缩压、舒张压、空腹血糖、空腹胰岛素、总胆固醇、甘油三酯、低密度脂蛋白、高密度脂蛋白,计算体质量指数(BMI)、腰臀比(WHR)、胰岛素抵抗指数(HOMA-IR)和动脉粥样硬化指数(AI);采用彩色多普勒超声仪观察各组儿童颈动脉内径(CADIA)、颈动脉内膜-中层厚度(CAIMT);比较MS组、肥胖非MS组和正常对照组各项指标。结果与正常对照组比较,MS组空腹血糖、空腹胰岛素、HOMA-IR、总胆固醇、甘油三酯、低密度脂蛋白升高,高密度脂蛋白降低,其中空腹血糖、空腹胰岛素、HOMA-IR、甘油三酯、高密度脂蛋白、低密度脂蛋白指标差异具有统计学意义(P<0.01或P<0.05);MS组与肥胖非MS组比较R-CAIMT、L-CAIMT增厚,差异具有统计学意义(P<0.01),R-CADIA、L-CADIA比较差异无统计学意义(P>0.05)。结论颈动脉超声参数和血液生化指标能提示 MS 儿童存在血管内皮功能损害,为MS 儿童心血管病变的早期发现和治疗提供客观依据。%Objective To investigate the association of ultrasound measurements of carotid artery and blood biochemical tests in children with metabolic syndrome(MS). Methods The study comprised 30 MS children, 30 obese children with non-MS and 30 normal weight children(control group). All participants underwent physical examination, ultrasound measurement of carotid artery and biochemical tests including systolic blood pressure(SBP), diastolic blood pressure(DBP), fasting plasma glucose(FPG), fasting insulin(FINS), total cholesterol(TC), triglycerides(TG), low density

  12. Salton Sea sampling program: baseline studies

    Energy Technology Data Exchange (ETDEWEB)

    Tullis, R.E.; Carter, J.L.; Langlois, G.W.


    Baseline data are provided on three species of fish from the Salton Sea, California. The fishes considered were the orange mouth corvina (Cynoscion xanthulus), gulf croaker (Bairdiella icistius) and sargo (Anisotremus davidsonii). Morphometric and meristic data are presented as a baseline to aid in the evaluation of any physiological stress the fish may experience as a result of geothermal development. Analyses were made on muscle, liver, and bone of the fishes sampled to provide baseline data on elemental tissue burdens. The elements measured were: As, Br, Ca, Cu, Fe, Ga, K, Mn, Mi, Pb, Rb, Se, Sr, Zn, and Zr. These data are important if an environmentally sound progression of geothermal power production is to occur at the Salton Sea.

  13. Baseline methodologies for clean development mechanism projects

    Energy Technology Data Exchange (ETDEWEB)

    Lee, M.K. (ed.); Shrestha, R.M.; Sharma, S.; Timilsina, G.R.; Kumar, S.


    The Kyoto Protocol and the Clean Development Mechanism (CDM) came into force on 16th February 2005 with its ratification by Russia. The increasing momentum of this process is reflected in more than 100 projects having been submitted to the CDM Executive Board (CDM-EB) for approval of the baselines and monitoring methodologies, which is the first step in developing and implementing CDM projects. A CDM project should result in a net decrease of GHG emissions below any level that would have resulted from other activities implemented in the absence of that CDM project. The 'baseline' defines the GHG emissions of activities that would have been implemented in the absence of a CDM project. The baseline methodology is the process/algorithm for establishing that baseline. The baseline, along with the baseline methodology, are thus the most critical element of any CDM project towards meeting the important criteria of CDM, which are that a CDM should result in 'real, measurable, and long term benefits related to the mitigation of climate change'. This guidebook is produced within the frame work of the United Nations Environment Programme (UNEP) facilitated 'Capacity Development for the Clean Development Mechanism (CD4CDM)' Project. This document is published as part of the projects effort to develop guidebooks that cover important issues such as project finance, sustainability impacts, legal framework and institutional framework. These materials are aimed to help stakeholders better understand the CDM and are believed to eventually contribute to maximize the effect of the CDM in achieving the ultimate goal of UNFCCC and its Kyoto Protocol. This Guidebook should be read in conjunction with the information provided in the two other guidebooks entitled, 'Clean Development Mechanism: Introduction to the CDM' and 'CDM Information and Guidebook' developed under the CD4CDM project. (BA)

  14. Risk of cardiovascular events among women with high normal blood pressure or blood pressure progression: prospective cohort study


    Conen, David; Ridker, Paul M.; Buring, Julie E.; Glynn, Robert J.


    Objective To compare cardiovascular risk among women with high normal blood pressure (130-9/85-9 mm Hg) against those with normal blood pressure (120-9/75-84 mm Hg) and those with baseline hypertension.

  15. Long-baseline Neutrino Oscillation at DUNE (United States)

    Worcester, Elizabeth; DUNE Collaboration Collaboration


    The Deep Underground Neutrino Experiment (DUNE) is a long-baseline neutrino oscillation experiment with primary physics goals of determining the neutrino mass hierarchy and measuring δc P with sufficient sensitivity to discover CP violation in neutrino oscillation. CP violation sensitivity in DUNE requires careful understanding of systematic uncertainty, with contributions expected from uncertainties in the neutrino flux, neutrino interactions, and detector effects. In this presentation, we will describe the expected sensitivity of DUNE to long-baseline neutrino oscillation parameters, how various aspects of the experimental design contribute to that sensitivity, and the planned strategy for constraining systematic uncertainty in these measurements.

  16. Neutrino Interactions and Long-Baseline Experiments

    CERN Document Server

    Mosel, Ulrich


    The extraction of neutrino mixing parameters and the CP-violating phase requires knowledge of the neutrino energy. This energy must be reconstructed from the final state of a neutrino-nucleus reaction since all long-baseline experiments use nuclear targets. This reconstruction requires detailed knowledge of the neutrino reactions with bound nucleons and of the final state interactions of hadrons with the nuclear environment. Quantum-kinetic transport theory can be used to build an event generator for this reconstruction that takes basic nuclear properties, such as binding, into account. Some examples are discussed that show the effects of nuclear interactions on observables in long-baseline experiments

  17. 40 CFR 80.90 - Conventional gasoline baseline emissions determination. (United States)


    ... using the facility's oxygenated individual baseline fuel parameter values for summer and winter (per... using the oxygenated individual baseline fuel parameter values for summer and winter (per § 80.91... baseline NOX emissions shall be determined using the baseline individual baseline fuel parameter values...

  18. Effects of Feed-Used Mannanase Transgenic Corn on Growth Performance, Carcass Performance and Blood Physiological and Biochemical Indexes of Broilers%饲用甘露聚糖酶转基因玉米对肉鸡生长性能、屠宰性能及血清生理生化指标的影响

    Institute of Scientific and Technical Information of China (English)

    孟昆; 陈桂兰; 刘国华; 杨培龙; 姚斌


    本试验旨在研究甘露聚糖酶转基因玉米对肉鸡生长性能、屠宰性能及血清生理生化指标的影响。试验选择1日龄爱拔益加( AA)肉鸡480只,随机分成4个组,每组6个重复,每个重复20只鸡。4个组安排如下:组Ⅰ为非转基因玉米的对照组,不添加甘露聚糖酶;组Ⅱ为非转基因玉米添加低剂量微生物来源甘露聚糖酶组,甘露聚糖酶活力500 U/kg;组Ⅲ为低剂量甘露聚糖酶转基因玉米组,甘露聚糖酶活力500 U/kg;组Ⅳ为高剂量甘露聚糖酶转基因玉米组,甘露聚糖酶活力5000 U/kg。试验期42 d。结果表明:与对照组相比,组Ⅲ和Ⅳ42日龄肉鸡平均日增重有显著提高( P<0.05),组Ⅳ料重比显著降低( P<0.05)。组Ⅳ42日龄肉鸡屠宰率和全净膛率显著高于组Ⅱ和对照组( P<0.05)。组Ⅱ、Ⅲ和Ⅳ21和42日龄肉鸡的血液生理生化指标与对照组均没有显著差异( P>0.05)。由此可见,饲粮中添加甘露聚糖酶转基因玉米能提高受试肉鸡的生长性能和屠宰性能,但对血液生理生化指标没有显著影响。%This experiment was conducted to investigate the effects of mannanase transgenic corn on growth performance, carcass performance and blood physiological and biochemical indexes of broilers.A total of 480 one-day-old Arbor Acres ( AA) broilers were randomly allocated to 4 groups, with 6 six replicates per group and 20 broilers per replicate.The four groups showed as follows: group Ⅰ, broilers fed non-transgenic corn without addition of mannanase as the control; group Ⅱ, broilers fed non-transgenic corn with addition of mi-crobial mannanase at low dosage of mannanase activity ( 500 U/kg);groupⅢ, broilers fed mannanase trans-genic corn with low dosage of mannanase activity (500 U/kg); and group Ⅳ, broilers fed mannanase trans-genic corn with high dose of mannase activity ( 5 000 U/kg) .The trial lasted for

  19. 产后灌服营养补充液对奶牛血液生化指标及生产性能的影响%The Effect of Drenching Nutrition Solutions on Blood Biochemical Index and Production Performance in Postpartum Dairy Cows

    Institute of Scientific and Technical Information of China (English)

    于洪江; 沈泰钰; 高三思; 黄宝银; 董志昊; 刘润琪; 何平; 杨威; 徐闯


    本试验旨在通过产后灌服营养补充液试验,反映出产后灌服营养补充液对产后奶牛血液生化指标和生产性能的影响,为牧场产后奶牛的保健措施提供理论依据与实际参考。选择二胎以上的健康奶牛14头,随机分成三组,试验一、二组各4头,对照组6头;试验一、二组奶牛分娩后灌服由丙二醇、红糖、食盐、反刍动物专用酵母、氯化镁组成的保健制剂,试验二组奶牛分娩后灌服由丙二醇、食盐、XP酵母培养物、丙酸钙、硫酸镁组成的保健制剂,对照组奶牛只灌服等量的30~50L、37℃温水。采集血清对BHBA、NEFA、AST、GLU、Ca、P和Mg指标进行检测;统计305d泌乳量、21d时泌乳量、首次发情天数以及疾病发生情况并进行比较分析。结果表明,本试验的两组保健制剂对奶牛产后酮病、脂肪肝具有一定的预防作用,试验组奶牛305d泌乳量、21d时泌乳量明显增多,首次发情天数缩短,血液生化指标变化趋于稳定的正常值。%The purpose of this study was to provide a theoretical basis and practical reference for health care measures of dairy cows during the transition with detecting the blood biochemical indicators for estimating the production of dairy cows. Fourteen cows (parity>2) were selected and divided into three groups, group I (n=4), group II (n=4), group III (n=6) respectively. Cows in group I and group II were feed health care preparation that consist of propylene glycol, brown sugar, calcium propionate, salt, yeast culture, magnesium chloride, and magnesium sulfate. cows was purfused same water with two groups. The contents of BHBA, NEFA, AST, GLU, Ca, P, Mg, K and Na in serum were detected. Milk yield of 305d milk production on day 21 postpartum, ifrst days in estrus and disease accident were collected through farm management software, then analyzed by using the statistical analysis. Results showed that health care

  20. Population Study of Urban, Rural, and Semiurban Regions for the Detection of Endovascular Disease and Prevalence of Risk Factors and Holistic Intervention Study: Rationale, Study Design, and Baseline Characteristics of PURSE-HIS. (United States)

    Thanikachalam, Sadagopan; Harivanzan, Vijayakumar; Mahadevan, Murugappapillai V; Murthy, J S N; Anbarasi, Chandrasekar; Saravanababu, Chidambaram S; Must, Aviva; Baliga, Ragavendra R; Abraham, William T; Thanikachalam, Mohan


    We designed and implemented the PURSE-HIS (Population Study of Urban, Rural and Semiurban Regions for the Detection of Endovascular Disease and Prevalence of Risk Factors and Holistic Intervention Study) to understand the prevalence and progression of subclinical and overt endovascular disease (EVD) and its risk factors in urban, semiurban, and rural communities in South India. The study is also designed to generate clinical evidence for effective, affordable, and sustainable community-specific intervention strategies to control risks factors for EVD. As of June 2012, 8,080 (urban: 2,221; semiurban: 2,821; rural: 3,038) participants >20 years of age were recruited using 2-stage cluster sampling. Baseline measurements included standard cardiovascular disease risk factors, sociodemographic factors, lifestyle habits, psychosocial factors, and nutritional assessment. Fasting blood samples were assayed for putative biochemical risk factors and urine samples for microalbuminuria. All nondiabetic participants underwent oral glucose tolerance test with blood and urine samples collected every 30 min for 2 h. Additional baseline measurements included flow-mediated brachial artery endothelial vasodilation, assessment of carotid intimal medial wall thickness using ultrasonography, screening for peripheral vascular disease using ankle and brachial blood pressures, hemodynamic screening using a high-fidelity applanation tonometry to measure central blood pressure parameters, and aortic pulse wave velocity. To assess prevalence of coronary artery disease, all participants underwent surface electrocardiography and documentation of ventricular wall motion abnormality and function using echocardiography imaging. To detect subclinical lesions, all eligible participants completed an exercise treadmill test. Prospectively, the study will assess progression of subclinical and overt EVD, including risk factor-outcome relation differences across communities. The study will also evaluate

  1. Effects of Chinese herbal medicine additives of anti-heat stress on production performance,egg quality and blood biochemical indexes of laying hens%中草药抗热应激剂对蛋鸡生产性能、蛋品质及血液生化指标的影响

    Institute of Scientific and Technical Information of China (English)



    【Objective】 The research was conducted to study the effects of Chinese herbal medicine additives of anti-heat stress on production performance,egg quality and blood biochemical indexes of laying hens in summer.【Method】 480 Roman hens of 310-day-old were randomly divided into 4 groups(120 hens in each group,4 replicates with 30 layers in each).Group I was fed the basal diet as control(CK).The experiment group Ⅱ,Ⅲ,Ⅳ were fed 4,8,12 g/kg Chinese herbal medicine additives of anti-heat stress on the basal diet,respectively.At the end of experiment,production performance,egg quality,blood biochemical indexes were measured.【Result】 Chinese herbal medicine additives of anti-heat stress increased feed intake,laying rate and average egg weight,decreased feed/egg ratio,egg broken ratio,mortality,increased eggshell thickness,eggshell strength,yolk color(P〈0.05).But egg shape index,egg specific density,haugh unit were not significantly affected.The serum TP,ALB,GLU,TG,Ca2+,K+,T3 and T4 were increased,concentration of serum cortisol and activity of AST,ALT,LDH,CK decreased,activity of ALP increased.【Conclusion】 Chinese herbal medicine additives of anti-heat stress could increase laying performance,egg quality,decrease mortality,and improve blood biochemical indexes in layers.%【目的】探讨中草药抗热应激剂对夏季蛋鸡生产性能、蛋品质及血液生化指标的影响。【方法】选取480只310日龄健康、体质量和生产性能相近的罗曼褐蛋鸡,随机分为4组,每组120只,每组设4个重复,每重复30只,I组为对照组(CK),饲喂基础日粮;Ⅱ、Ⅲ、Ⅳ组为试验组,分别在基础日粮中添加4,8,12g/kg自制中草药抗热应激剂,分析其对蛋鸡生产性能、蛋品质及血液生化指标的影响。【结果】夏季在日粮中添加中草药抗热应激剂,可显著提高蛋鸡的采食量、产蛋率、平均蛋质量,降低料蛋比、破蛋率和死淘率;改善蛋壳厚度

  2. 管花肉苁蓉醇提物对大鼠血液生化指标及心血管功能的影响%Effect of Cistanche tubulosa Ethanol Extract on Blood Biochemical Parameters and Cardiovascular Function in Rats

    Institute of Scientific and Technical Information of China (English)

    吾买尔江·牙合甫; 赵红琼; 冯亮; 夏瑞阳; 郑晓风; 张琦; 张晓红; 姚刚


    旨在探讨管花肉苁蓉醇提物对大鼠血液生化指标及心血管功能的影响,为管花肉苁蓉对心血管功能不全的辅助治疗作用提供现代医学研究佐证。将24只大鼠随机分为低、中、高剂量组和对照组,分别灌胃0.1、0.2、0.3 g/(100 g·BW)的管花肉苁蓉乙醇提取物和同体积的生理盐水,连续给药30 d。试验结束时,测定并比较各给药组与对照组的主要血液生化指标、心血管功能相关酶和激素水平、脏器系数以及颈动脉平滑肌的运动指标。结果表明,低剂量管花肉苁蓉醇提物显著提高大鼠血清ALT含量(P<0.05),但低、中、高剂量管花肉苁蓉醇提物对其他血液生化指标影响均不显著(P>0.05);对大鼠3种心肌酶活力的影响不显著(P>0.05);低、中剂量组的Ang-Ⅱ水平显著低于对照组(P<0.05),但EPO活力、ALD水平的变化不显著(P>0.05);对大鼠的增重率、脏器系数影响不显著(P>0.05),对颈动脉平滑肌运动指标有一定的降低趋势(P>0.05)。综上提示,总体来看,管花肉苁蓉醇提物对大鼠的血液生化指标及心血管功能有一定的影响。%This study aimed to assess the effect of Cistanche tubulosa ethanol extract on blood biochemical parameters and cardiovascular function in rats and to evidence the assistant therapy action of Cistanche tubulosa on cardiovascular insufficiency from the aspect of modern medicine. A total of 24 rats were randomly assigned to 4 groups. The low, moderate and high dosage groups were administered intragastrically 0.1, 0.2 and 0.3 g/(100 g·BW) of Cistanche tubulosa ethanol extract, and the control group was administered intragastrically the same volume of normal saline. The experimental period lasted for 30 days. At the end of the experiment, content of the main blood biochemical parameters, activity or content of the enzyme and hormone associated with

  3. BEST: Biochemical Engineering Simulation Technology

    Energy Technology Data Exchange (ETDEWEB)


    The idea of developing a process simulator that can describe biochemical engineering (a relatively new technology area) was formulated at the National Renewable Energy Laboratory (NREL) during the late 1980s. The initial plan was to build a consortium of industrial and U.S. Department of Energy (DOE) partners to enhance a commercial simulator with biochemical unit operations. DOE supported this effort; however, before the consortium was established, the process simulator industry changed considerably. Work on the first phase of implementing various fermentation reactors into the chemical process simulator, ASPEN/SP-BEST, is complete. This report will focus on those developments. Simulation Sciences, Inc. (SimSci) no longer supports ASPEN/SP, and Aspen Technology, Inc. (AspenTech) has developed an add-on to its ASPEN PLUS (also called BioProcess Simulator [BPS]). This report will also explain the similarities and differences between BEST and BPS. ASPEN, developed by the Massachusetts Institute of Technology for DOE in the late 1970s, is still the state-of-the-art chemical process simulator. It was selected as the only simulator with the potential to be easily expanded into the biochemical area. ASPEN/SP, commercially sold by SimSci, was selected for the BEST work. SimSci completed work on batch, fed-batch, and continuous fermentation reactors in 1993, just as it announced it would no longer commercially support the complete ASPEN/SP product. BEST was left without a basic support program. Luckily, during this same time frame, AspenTech was developing a biochemical simulator with its version of ASPEN (ASPEN PLUS), which incorporates most BEST concepts. The future of BEST will involve developing physical property data and models appropriate to biochemical systems that are necessary for good biochemical process design.

  4. Solid Waste Program technical baseline description

    Energy Technology Data Exchange (ETDEWEB)

    Carlson, A.B.


    The system engineering approach has been taken to describe the technical baseline under which the Solid Waste Program is currently operating. The document contains a mission analysis, function analysis, system definition, documentation requirements, facility and project bases, and uncertainties facing the program.

  5. How Valid Are the Portland Baseline Essays? (United States)

    Martel, Erich


    Portland, Oregon's "African-American Baseline Essays," widely used in creating multicultural curricula, inaccurately depicts ancient Egyptians as black people and Olmec civilization as derived from African influences. The authors advance racial theories long abandoned by mainline Africa scholars, attribute mystical powers to pyramids,…

  6. Waste management project technical baseline description

    Energy Technology Data Exchange (ETDEWEB)

    Sederburg, J.P.


    A systems engineering approach has been taken to describe the technical baseline under which the Waste Management Project is currently operating. The document contains a mission analysis, function analysis, requirement analysis, interface definitions, alternative analysis, system definition, documentation requirements, implementation definitions, and discussion of uncertainties facing the Project.

  7. Guidance on Port Biological Baseline Surveys (PBBS)

    Digital Repository Service at National Institute of Oceanography (India)

    Awad, A.; Haag, F.; Anil, A.C.; Abdulla, A.

    This publication has been prepared by GBP, IOI, CSIR-NIO and IUCN in order to serve as guidance to those who are planning to carry out a port biological baseline survey, in particular in the context of Ballast Water Management. It has been drafted...

  8. Rationing in the presence of baselines

    DEFF Research Database (Denmark)

    Hougaard, Jens Leth; Moreno-Ternero, Juan D.; Østerdal, Lars Peter


    We analyze a general model of rationing in which agents have baselines, in addition to claims against the (insufficient) endowment of the good to be allocated. Many real-life problems fit this general model (e.g., bankruptcy with prioritized claims, resource allocation in the public health care...

  9. [Renal lithiasis. Biochemical changes in the follow-up]. (United States)

    Spivacow, Francisco R; del Valle, Elisa E; Zancheta, José R


    With the aim of assessing if biochemical changes occur in the follow up of patients with renal lithiasis, 237 patients were studied (115 women and 122 men, mean age 39 +/- 8 and 42 +/- 7 years, respectively) and controlled during 27.3 +/- 19.3 months. All of them had previously undergone metabolic evaluations at baseline and one or more than one control studies afterwards. Patients with a diagnosis of sponge kidney, renal tubular acidosis, primary hyperparathyroidism, anatomical malformations of the urinary tract, or urinary infections were not included. Two populations were identified: those who presented changes in the baseline diagnosis (139 patients, Group I) and those who presented no changes (98 patients, Group II). In these groups, no differences were observed in baseline metabolic diagnoses or in the follow-up period. Hypocitraturia was the additional diagnosis most frequently observed (43.1%), followed by Idiopatic hypercalciuria (20.8%) and abnormalities of uric acid (16.5%). In the group of 110 patients followed up for more than 3 years, 37 patients recurred (33%). Among the latter, 25 (23%) changed the baseline metabolic diagnosis vs. 12 (11%) that maintained the same diagnosis (p < 0.002). Changes in metabolic disorders were frequently observed in the follow up of patients with nephrolithiasis. These changes are not necessarily associated with the diet indicated or drug treatment. Hypocitraturia was the additional metabolic disorder most frequently found. In general, there is a higher recurrence rate in those patients who present changes in their biochemical parameters and undergo no treatment.

  10. Blood Donation (United States)

    Tests and Procedures Blood donation By Mayo Clinic Staff Blood donation is a voluntary procedure. You agree to have blood drawn so that it can ... have a disease that requires blood components. Blood donation makes all of this possible. There are several ...

  11. 妊娠后期营养限饲蒙古绵羊对其胎儿生长发育及血液生理生化指标的影响%Maternal Undernutrition during Late Pregnancy: Effects on Growth Development and Physiological and Biochemical Indexes in Blood of Mongolia Ovine Fetus

    Institute of Scientific and Technical Information of China (English)

    张崇志; 刘迎春; 高峰; 曹平; 侯先志; 李士栋


    This study was carried out to investigate the effects of maternal undernutrition during late pregnancy on growth development and physiological and biochemical indexes in blood of Mongolia ovine fetus. A total of 42 healthy Mongolia ewes mated at a synchronized estrus were selected. Six ewes were slaughtered at the beginning of the experiment (90 d of gestation) and the remained animals were allocated to three metabolizable energy (ME) level groups; restricted group 1 [RG1, 0.175 MJ/(kg BW0.75 · d) , n = 14] , restricted group 2 [RG2, 0.330 MJ/(kg BW0.75· d), n =12] and control group [CG, ad libitum, 0.670 MJ/(kg BW0.75 · d), n = 10]. At 140 d of gestation, six ewes in each group were slaughtered, quickly removed the fetus, and measured the fetal body weight, body measurements, physiological and biochemical indexes in blood. The results showed as follows; fetal weight ( P < 0. 01) , body length ( P < 0. 05) , chest circumference ( P < 0. 05) , abdominal circumference (P <0. 05) , curved crown-rump length (P <0. 01) , red blood cell count (P<0.01) , hemoglobin content (P<0.01) , hematokrit (P<0.01) , total amino acid content (P<0.05) , total antioxidation capacity (P < 0. 01) and superoxide dismutase activity (P < 0. 05) were significantly decreased and average platelet volume (P <0. 05) , coefficient of variation of red blood cell distribution width (P <0. 05) , nonesterified fatty acid content (P <0. 05) , p-hydroxybutyrate content (P <0.01) , glutathione peroxidase activity (P <0. 05) and malonaldehyde content (P <0. 05) were significantly enhanced in RG1 compared with CG. For RG2, the decrease of fetal body weight (P <0. 01) and total antioxidation capacity (P <0.05) was founded and average platelet volume was increased compared with CG (P <0.05). In conclusion , the fetal growth and development are affected seriously by maternal undernutrition during late pregnancy , which leads to severe malnourished anaemia, nutritional and metabolic disorder, and oxidative

  12. Chaotic advection in blood flow. (United States)

    Schelin, A B; Károlyi, Gy; de Moura, A P S; Booth, N A; Grebogi, C


    In this paper we argue that the effects of irregular chaotic motion of particles transported by blood can play a major role in the development of serious circulatory diseases. Vessel wall irregularities modify the flow field, changing in a nontrivial way the transport and activation of biochemically active particles. We argue that blood particle transport is often chaotic in realistic physiological conditions. We also argue that this chaotic behavior of the flow has crucial consequences for the dynamics of important processes in the blood, such as the activation of platelets which are involved in the thrombus formation.

  13. A New Slow-Release Non-Protein Nitrogen:Effects of Supplemental Level on Lactation Performance and Blood Biochemical Indices of Lactating Goats%一种新型缓释非蛋白氮添加水平对奶山羊泌乳性能及血液生化指标的影响

    Institute of Scientific and Technical Information of China (English)

    王会; 罗军; 张伟; 张天颖; 杨地坤


    本试验旨在探讨饲粮中添加不同水平的一种新型缓释非蛋白氮( srNPN)对奶山羊泌乳性能及血液生化指标的影响。选择2~3胎平均体重约为60 kg的健康西农萨能奶山羊泌乳羊48只,随机分为4组,每组12只。各组饲粮 srNPN 添加水平分别为0(对照组)、0.25%、0.50%、0.75%。预试期1周,正试期16周。结果表明:1)饲粮中添加不同水平的srNPN对奶山羊日均采食量无显著影响( P>0.05)。2)饲粮中添加srNPN可显著提高奶山羊日均产奶量(P0.05);各组乳中乳蛋白、乳糖及乳非脂固形物含量差异不显著( P>0.05)。4)饲粮中添加srNPN可显著降低血液尿素氮含量(P0.05)。由此得出,饲粮中添加srNPN能够提高奶山羊乳中乳脂含量,降低血液尿素氮含量,添加水平为0.50%时奶山羊日均产奶量最高。%This experiment was conducted to investigate the effects of different supplemental level of a new slow-release non-protein nitrogen ( srNPN ) on lactation performance and blood biochemical indices of dairy goats. Forty-eight healthy lactating Xinong Saanen dairy goats with 2 to 3 parities and about 60 kg of average body weight were randomly assigned to 4 groups with 12 goats in each group. Goats in the 4 groups were fed diets supplemented with srNPN at 0, 0. 25%, 0. 50% and 0. 75%, respectively. There was a pretrial period of 1 week followed by an experimental period of 16 weeks. The results showed as follows:1 ) dietary supple-mentation of srNPN at different levels did not affect average daily feed intake of lactating goats ( P>0 . 05 ) . 2 ) The supplementation of srNPN significantly increased average daily milk yield ( P 0. 05); besides, the contents of milk protein, lac-tose and milk solids-non-fat were not significantly affected by the supplementation of srNPN ( P>0 . 05 ) . 4 ) The supplementation of srNPN could significantly decrease blood urea nitrogen content ( P0. 05). In conclusion, dietary supplementation of sr

  14. CASA Uno GPS orbit and baseline experiments (United States)

    Schutz, B. E.; Ho, C. S.; Abusali, P. A. M.; Tapley, B. D.


    CASA Uno data from sites distributed in longitude from Australia to Europe have been used to determine orbits of the GPS satellites. The characteristics of the orbits determined from double difference phase have been evaluated through comparisons of two-week solutions with one-week solutions and by comparisons of predicted and estimated orbits. Evidence of unmodeled effects is demonstrated, particularly associated with the orbit planes that experience solar eclipse. The orbit accuracy has been assessed through the repeatability of unconstrained estimated baseline vectors ranging from 245 km to 5400 km. Both the baseline repeatability and the comparison with independent space geodetic methods give results at the level of 1-2 parts in 100,000,000. In addition, the Mojave/Owens Valley (245 km) and Kokee Park/Ft. Davis (5409 km) estimates agree with VLBI and SLR to better than 1 part in 100,000,000.

  15. Dissipative Effect in Long Baseline Neutrino Experiments

    CERN Document Server

    Oliveira, Roberto L N


    The propagation of neutrinos in long baselines experiments may be influenced by dissipation effects. Using Lindblad Master Equation we evolve neutrinos taking into account these dissipative effects. The MSW and the dissipative effects may change the probabilities behavior. In this work, we show and explain how the behavior of the probabilities can change due to the decoherence and relaxation effects acting individually with the MSW effect. A new exotic peak appears in this case and we show the difference between the decoherence and relaxation effects in the appearance of this peak. We also adapt the usual approximate expression for survival and appearance probabilities with all possible decoherence effects. We suppose the baseline of DUNE and show how each decoherence parameters change the probabilities analyzing the possible modification using numeric and analytic approach.

  16. Joint Multi-baseline SAR Interferometry

    Directory of Open Access Journals (Sweden)

    S. Tebaldini


    Full Text Available We propose a technique to provide interferometry by combining multiple images of the same area. This technique differs from the multi-baseline approach in literature as (a it exploits all the images simultaneously, (b it performs a spectral shift preprocessing to remove most of the decorrelation, and (c it exploits distributed targets. The technique is mainly intended for DEM generation at centimetric accuracy, as well as for differential interferometry. The problem is framed in the contest of single-input multiple-output (SIMO channel estimation via the cross-relations (CR technique and the resulting algorithm provides significant improvements with respect to conventional approaches based either on independent analysis of single interferograms or multi-baselines phase analysis of single pixels of current literature, for those targets that are correlated in all the images, like for long-term coherent areas, or for acquisitions taken with a short revisit time (as those gathered with future satellite constellations.

  17. Donating Blood (United States)

    ... can't get an infection or disease from giving blood. The needles and other equipment used are sterile ... part of blood (plasma) within 72 hours after giving blood. It generally takes about 4–8 weeks to ...

  18. The Gambia Impact Evaluation Baseline Report



    The Government of The Gambia is implementing the Maternal and Child Nutrition and Health Results Project (MCNHRP) to increase the utilization of community nutrition and primary maternal and child health services. In collaboration with the Government, the World Bank is conducting an impact evaluation (IE) to assess the impact of the project on key aspects of maternal and child nutrition and health. The baseline survey for the MCNHRP IE took place between November 2014 and February 2015. It c...

  19. Systematic errors in long baseline oscillation experiments

    Energy Technology Data Exchange (ETDEWEB)

    Harris, Deborah A.; /Fermilab


    This article gives a brief overview of long baseline neutrino experiments and their goals, and then describes the different kinds of systematic errors that are encountered in these experiments. Particular attention is paid to the uncertainties that come about because of imperfect knowledge of neutrino cross sections and more generally how neutrinos interact in nuclei. Near detectors are planned for most of these experiments, and the extent to which certain uncertainties can be reduced by the presence of near detectors is also discussed.

  20. Efficient Wide Baseline Structure from Motion (United States)

    Michelini, Mario; Mayer, Helmut


    This paper presents a Structure from Motion approach for complex unorganized image sets. To achieve high accuracy and robustness, image triplets are employed and (an approximate) camera calibration is assumed to be known. The focus lies on a complete linking of images even in case of large image distortions, e.g., caused by wide baselines, as well as weak baselines. A method for embedding image descriptors into Hamming space is proposed for fast image similarity ranking. The later is employed to limit the number of pairs to be matched by a wide baseline method. An iterative graph-based approach is proposed formulating image linking as the search for a terminal Steiner minimum tree in a line graph. Finally, additional links are determined and employed to improve the accuracy of the pose estimation. By this means, loops in long image sequences are implicitly closed. The potential of the proposed approach is demonstrated by results for several complex image sets also in comparison with VisualSFM.

  1. Valores de referência de parâmetros bioquímicos no sangue de duas linhagens de camundongos Reference values for blood-based biochemical parameters in BALB/C and C57BL/6 wild-type mice

    Directory of Open Access Journals (Sweden)

    Aline Silva Almeida


    Full Text Available INTRODUÇÃO: Conhecer os parâmetros bioquímicos individuais de animais de laboratório utilizados na experimentação é importante, pois eles servirão como parâmetros para avaliar alterações funcionais em órgãos e como base para estabelecer valores de referência. OBJETIVO:Estabelecer valores de referência bioquímicos do sangue em camundongos das linhagens BALB/c e C57BL/6 selvagens do Biotério da Disciplina de Biologia Celular da Universidade Federal do Triângulo Mineiro (UFTM. Materiais e métodos: Foram utilizados 30 camundongos (BALB/c e C57BL/6 selvagem. Os exames realizados foram glicose, triglicérides, colesterol, proteínas totais, albumina, amilase, ácido úrico, uréia, fosfatase alcalina (kits Wiener, e as determinações foram realizadas no equipamento BIOPLUS-2000. RESULTADOS:Entre os nove analitos observou-se que quatro (albumina, glicose, proteínas totais e colesterol apresentaram diferenças estatisticamente significativas entre as linhagens. Padronizamos como valores de referência para os camundongos os valores do intervalo de confiança (IC. Nos analitos em que houve diferença significativa entre as linhagens (p INTRODUCTION: Identifying individual biochemical parameters of laboratory animal species is important inasmuch as they may be used in the evaluation of functional changes in organs and in the establishment of reference values. OBJECTIVE: To establish biochemical reference values for blood tests in BALB/c and C57BL/6 wild-type mice from the Vivarium of the Department of Cellular Biology at the Federal University of "Triângulo Mineiro". MATERIALS AND METHODS: Thirty wild-type mice of the lineages BALB/c and C57BL/6 were used to evaluate the serum levels of glucose, triglycerides, cholesterol, total protein, albumin, amylase, uric acid, urea and alkaline phosphatase. The determinations were performed in a BIOPLUS-2000 analyzer. Results: Four out of the nine analytes (albumin, glucose, total proteins and

  2. Molecular and biochemical studies on bovine ephemeral fever

    Directory of Open Access Journals (Sweden)

    Nahed S. Thabet; Emad W. Ghazy; Mohamed A. Nayel; Mohamed Abo-Elkhair


    Full Text Available Bovine ephemeral fever (BEF in cattle has been reported to be associated with a range of biochemical changes which are similar to those seen in milk fever. This study aimed to clarify the biochemical alterations that associate infection of cattle with BEF with special references to the mechanisms involved in the development of hypocalcemia. The study was conducted on 30 cases of cattle infected with BEF based on the characteristic clinical signs which were confirmed by isolation of virus and RT-PCR. Another 6 healthy cows were used in the study as control. The evaluated parameters included biochemical variables such as serum values of total protein (TP, albumin (Alb, glucose (Glu, total calcium (tCa, ionized calcium (iCa, inorganic phosphorus (P, magnesium (Mg, sodium (Na, potassium (K, chloride (Cl, creatinine (Cr, blood urea nitrogen (BUN and serum activity of alkaline phosphatase (ALP. Hormonal profile included parathyroid hormone (PTH, insulin (Ins, and cortisol (Cor. The results showed that BEF-infected animals demonstrated a significant decrease (P<0.05 in serum concentrations of TP, Glo, iCa, P, Na, K, BUN and ALP while the mean values of serum levels of Glu and Cl were significantly increased (P<0.05. The mean values of serum levels of PTH were significantly decreased (P<0.05 while serum concentrations of Ins and Cor showed a significant increase. It was concluded that the clinical signs of bovine ephemeral fever are related to the hypocalcemia resulting from suppression of parathyroid hormone which seems to be mediated by respiratory alkalosis caused by the disease. This explanation needs future studies to provide a direct link between measurement of blood indicators of acid-base status, blood biochemical parameters and urine analysis. However, this work can provide a good knowledge about the pathogenesis of the disease that can lead to better management and proper treatment.

  3. Baseline Response Levels Are a Nuisance in Infant Contingency Learning (United States)

    Millar, W. S.; Weir, Catherine


    The impact of differences in level of baseline responding on contingency learning in the first year was examined by considering the response acquisition of infants classified into baseline response quartiles. Whereas the three lower baseline groups showed the predicted increment in responding to a contingency, the highest baseline responders did…

  4. Effect of freezing and storage time of blood serum of lambs in the determination of biochemical parametersEfeito do congelamento e do tempo de armazenamento do soro sanguíneo de cordeiros na determinação de parâmetros bioquímicos

    Directory of Open Access Journals (Sweden)

    Larissa Emy Ishiy


    Full Text Available This study aimed to evaluate the effect of freezing (- 20 ºC and time of storage of serum in the determination of biochemical values in sheep. It was held at the Universidade Estadual do Centro-Oeste in Guarapuava – PR. The serum was obtained from 32 Corriedale x Ille de France crossbred lambs, four months old. Nine hundred and sixty tests were performed, the first immediately after sampling (time zero, after 7, 14, 21, 28 days after blood collection. The analytes tested were aspartate aminotranferase (AST, urea, total protein, cholesterol, phosphorus and magnesium. The results showed that the time of storage of frozen samples (-20 ºC was able to alter results of total protein, phosphorus, and magnesium. The serum measurements of AST, cholesterol and urea did not differ in different storage times.Este trabalho teve como objetivo avaliar o efeito do congelamento (- 20 ºC e o tempo de armazenamento do soro na determinação de valores bioquímicos em ovinos. Foi realizado na Universidade Estadual do Centro-Oeste em Guarapuava – PR. O soro foi obtido de 32 cordeiros da raça Ile de France x Corriedale, com 4 meses de idade. Foram realizadas análises, imediatamente após a obtenção da amostra, tempo zero e após 7, 14, 21, 28 dias da colheita do sangue. Os analitos testados foram aspartato-amino-transferase (AST, uréia, proteína total, colesterol, fósforo e magnésio. Os resultados permitiram concluir que o tempo de armazenamento das amostras congeladas (- 20 ºC causou alterações nos resultados para as análises de proteína total, fósforo, e magnésio. As determinações séricas AST, colesterol e uréia não diferiram estatisticamente nos diferentes tempos de armazenamento.

  5. Associative learning in biochemical networks


    Ghandi, Nikhil; Ashkenasy, Gonen; Tannenbaum, Emmanuel


    We develop a simple, chemostat-based model illustrating how a process analogous to associative learning can occur in a biochemical network. Associative learning is a form of learning whereby a system "learns" to associate two stimuli with one another. In our model, two types of replicating molecules, denoted A and B, are present in some initial concentration in the chemostat. Molecules A and B are stimulated to replicate by some growth factors, denoted GA and GB, respectively. It is also assu...

  6. Biochemical markers of bone turnover

    Energy Technology Data Exchange (ETDEWEB)

    Kim, Deog Yoon [College of Medicine, Kyunghee Univ., Seoul (Korea, Republic of)


    Biochemical markers of bone turnover has received increasing attention over the past few years, because of the need for sensitivity and specific tool in the clinical investigation of osteoporosis. Bone markers should be unique to bone, reflect changes of bone less, and should be correlated with radiocalcium kinetics, histomorphometry, or changes in bone mass. The markers also should be useful in monitoring treatment efficacy. Although no bone marker has been established to meet all these criteria, currently osteocalcin and pyridinium crosslinks are the most efficient markers to assess the level of bone turnover in the menopausal and senile osteoporosis. Recently, N-terminal telopeptide (NTX), C-terminal telopeptide (CTX) and bone specific alkaline phosphatase are considered as new valid markers of bone turnover. Recent data suggest that CTX and free deoxypyridinoline could predict the subsequent risk of hip fracture of elderly women. Treatment of postmenopausal women with estrogen, calcitonin and bisphosphonates demonstrated rapid decrease of the levels of bone markers that correlated with the long-term increase of bone mass. Factors such as circadian rhythms, diet, age, sex, bone mass and renal function affect the results of biochemical markers and should be appropriately adjusted whenever possible. Each biochemical markers of bone turnover may have its own specific advantages and limitations. Recent advances in research will provide more sensitive and specific assays.

  7. Evaluation of biological and biochemical quality of whey protein. (United States)

    Haraguchi, Fabiano Kenji; Pedrosa, Maria Lucia; Paula, Heberth de; Santos, Rinaldo Cardoso dos; Silva, Marcelo Eustáquio


    Nutritional and biochemical properties of noncommercial whey protein have been described since 1950. However, comparisons between commercial whey protein for human consumption and casein are rarely found. The aim of this study was to compare biological quality of a commercial whey protein with casein and its effect on biochemical parameters of rats. Thirty-two weanling Fisher rats were divided into three groups and given the following diets: casein group, standard diet (AOAC); whey protein group, modified AOAC diet with whey protein instead of casein; and casein:whey group, modified AOAC diet with 70%:30% casein:whey. A protein-free group was used for determination of endogenous nitrogen losses. Net protein ratio, protein efficiency ratio, and true digestibility were determined, and blood was collected for biochemical analysis. When compared with casein, whey protein showed significant differences for all biological parameters evaluated, as well as for albumin, total protein, total cholesterol, and glucose concentrations. Replacing 30% of casein with whey protein did not affect these parameters. A positive relation among whey protein, high-density lipoprotein-cholesterol, and paraoxonase activity was found. Hepatic or renal dysfunctions were not observed. In conclusion, in comparison with casein, commercial whey protein had higher values of biological parameters, and biochemical evaluation revealed it improved glycemic homeostasis, lipid status, and paraoxonase activity in rats.

  8. Development of a new first-aid biochemical detector (United States)

    Hu, Jingfei; Liao, Haiyang; Su, Shilin; Ding, Hao; Liu, Suquan


    The traditional biochemical detector exhibits poor adaptability, inconvenient carrying and slow detection, which can't meet the needs of first-aid under field condition like natural or man-made disasters etc. Therefore a scheme of first-aid biochemical detector based on MOMES Micro Spectrometer, UV LED and Photodiode was proposed. An optical detection structure combined continuous spectrum sweep with fixed wavelength measurement was designed, which adopted mobile detection optical path consisting of Micro Spectrometer and Halogen Lamp to detect Chloride (Cl-), Creatinine (Cre), Glucose (Glu), Hemoglobin (Hb). The UV LED and Photodiode were designed to detect Potassium (K-), Carbon dioxide (CO2), Sodium (Na+). According to the field diagnosis and treatment requirements, we designed the embedded control hardware circuit and software system, the prototype of first-aid biochemical detector was developed and the clinical trials were conducted. Experimental results show that the sample's absorbance repeatability is less than 2%, the max coefficient of variation (CV) in the batch repeatability test of all 7 biochemical parameters in blood samples is 4.68%, less than the clinical requirements 10%, the correlation coefficient (R2) in the clinical contrast test with AU5800 is almost greater than 0.97. To sum up, the prototype meets the requirements of clinical application.

  9. Blood flow and microgravity (United States)

    Bureau, Lionel; Coupier, Gwennou; Dubois, Frank; Duperray, Alain; Farutin, Alexander; Minetti, Christophe; Misbah, Chaouqi; Podgorski, Thomas; Tsvirkun, Daria; Vysokikh, Mikhail


    The absence of gravity during space flight can alter cardio-vascular functions partially due to reduced physical activity. This affects the overall hemodynamics, and in particular the level of shear stresses to which blood vessels are submitted. Long-term exposure to space environment is thus susceptible to induce vascular remodeling through a mechanotransduction cascade that couples vessel shape and function with the mechanical cues exerted by the circulating cells on the vessel walls. Central to such processes, the glycocalyx - i.e. the micron-thick layer of biomacromolecules that lines the lumen of blood vessels and is directly exposed to blood flow - is a major actor in the regulation of biochemical and mechanical interactions. We discuss in this article several experiments performed under microgravity, such as the determination of lift force and collective motion in blood flow, and some preliminary results obtained in artificial microfluidic circuits functionalized with endothelium that offer interesting perspectives for the study of the interactions between blood and endothelium in healthy condition as well as by mimicking the degradation of glycocalyx caused by long space missions. A direct comparison between experiments and simulations is discussed. xml:lang="fr"

  10. Baseline Hematologic Results for Free-ranging White Rhinoceros (Ceratotherium simum) in Kruger National Park, South Africa. (United States)

    Miller, Michele; Buss, Peter; Wanty, Rachel; Parsons, Sven; van Helden, Paul; Olea-Popelka, Francisco


    Complete blood counts (n = 115) and red blood cell analytes (n = 80) were assessed in free-ranging white rhinoceros (Ceratotherium simum) from Kruger National Park (KNP), South Africa. Blood was collected from healthy animals immobilized between February and September 2009-11 for management purposes. Our objectives were to establish baselines for KNP's white rhinoceros population and to compare results based on sex and age group. Significant differences in total white blood cells, total eosinophils, and hemoglobin were found between adult and subadult rhinoceros. Female rhinoceros had significantly higher total white blood cells and lower hemoglobin compared with males. Hematologic analytes were similar to those published for other rhinoceros populations, although the impact of capture and sampling methods, nutritional status, and habitat should be considered when comparing data. This baseline hematology for healthy free-ranging white rhinoceros in KNP may be useful in assessing health status for translocation and medical interventions, including treatment of poaching cases.

  11. Very Long Baseline Interferometry with the SKA

    CERN Document Server

    Paragi, Zsolt; Reynolds, Cormac; Rioja, Maria; Deller, Adam; Zhang, Bo; Gurvits, Leonid; Bietenholz, Michael; Szomoru, Arpad; Bignall, Hayley; Boven, Paul; Charlot, Patrick; Dodson, Richard; Frey, Sandor; Garrett, Michael; Imai, Hiroshi; Lobanov, Andrei; Reid, Mark; Ros, Eduardo; van Langevelde, Huib; Zensus, J Anton; Zheng, Xing Wu; Alberdi, Antxon; Agudo, Ivan; An, Tao; Argo, Megan; Beswick, Rob; Biggs, Andy D; Brunthaler, Andreas; Campbell, Robert M; Cimo, Giuseppe; Colomer, Francisco; Corbel, Stephane; Conway, John; Cseh, David; Deane, Roger; Falcke, Heino; Gabanyi, Krisztina; Gawronski, Marcin; Gaylard, Michael; Giovannini, Gabriele; Giroletti, Marcello; Goddi, Ciriaco; Goedhart, Sharmila; Gomez, Jose L; Gunn, Alastair; Jung, Taehyun; Kharb, Preeti; Klockner, Hans-Rainer; Kording, Elmar; Kovalev, Yurii Yu; Kunert-Bajraszewska, Magdalena; Lindqvist, Michael; Lister, Matt; Mantovani, Franco; Marti-Vidal, Ivan; Mezcua, Mar; McKean, John; Middelberg, Enno; Miller-Jones, James; Moldon, Javier; Muxlow, Tom; O'Brien, Tim; Pérez-Torres, Miguel; Pogrebenko, Sergei; Quick, Jonathan; Rushton, Anthony P; Schilizzi, Richard; Smirnov, Oleg; Sohn, Bong Won; Surcis, Gabriele; Taylor, Greg; Tingay, Steven; Tudose, Valeriu; van der Horst, Alexander; van Leeuwen, Joeri; Venturi, Tiziana; Vermeulen, Rene; Vlemmings, Wouter; de Witt, Aletha; Wucknitz, Olaf; Yang, Jun


    Adding VLBI capability to the SKA arrays will greatly broaden the science of the SKA, and is feasible within the current specifications. SKA-VLBI can be initially implemented by providing phased-array outputs for SKA1-MID and SKA1-SUR and using these extremely sensitive stations with other radio telescopes, and in SKA2 by realising a distributed configuration providing baselines up to thousands of km, merging it with existing VLBI networks. The motivation for and the possible realization of SKA-VLBI is described in this paper.

  12. Optimization of the CLIC Baseline Collimation System

    Energy Technology Data Exchange (ETDEWEB)

    Resta-Lopez, Javier; /Oxford U., JAI; Angal-Kalinin, Deepa; /Daresbury; Fernandez-Hernando, Juan; /Daresbury; Jackson, Frank; /Daresbury; Dalena, Barbara; /CERN; Schulte, Daniel; /CERN; Tomas, Rogelio; /CERN; Seryi, Andrei; /SLAC


    Important efforts have recently been dedicated to the improvement of the design of the baseline collimation system of the Compact Linear Collider (CLIC). Different aspects of the design have been optimized: the transverse collimation depths have been recalculated in order to reduce the collimator wakefield effects while maintaining a good efficiency in cleaning the undesired beam halo; the geometric design of the spoilers have also been reviewed to minimize wakefields; in addition, the optics design have been polished to improve the collimation efficiency. This paper describes the current status of the CLIC collimation system after this optimization.

  13. SRP baseline hydrogeologic investigation, Phase 2

    Energy Technology Data Exchange (ETDEWEB)

    Bledsoe, H.W.


    As discussed in the program plan for the Savannah River Plant (SRP) Baseline Hydrogeologic Investigation, this program has been implemented for the purpose of updating and improving the current state of knowledge and understanding of the hydrogeologic systems underlying the Savannah River Plant (SRP). The objective of the program is to install a series of observation well clusters (wells installed in each major water bearing formation at the same site) at key locations across the plant site in order to: (1) provide detailed information on the lithology, stratigraphy, and groundwater hydrology, (2) provide observation wells to monitor the groundwater quality, head relationships, gradients, and flow paths.

  14. SRP Baseline Hydrogeologic Investigation, Phase 3

    Energy Technology Data Exchange (ETDEWEB)

    Bledsoe, H.W.


    The SRP Baseline Hydrogeologic Investigation was implemented for the purpose of updating and improving the knowledge and understanding of the hydrogeologic systems underlying the SRP site. Phase III, which is discussed in this report, includes the drilling of 7 deep coreholes (sites P-24 through P-30) and the installation of 53 observation wells ranging in depth from approximately 50 ft to more than 970 ft below the ground surface. In addition to the collection of geologic cores for lithologic and stratigraphic study, samples were also collected for the determination of physical characteristics of the sediments and for the identification of microorganisms.

  15. SRP baseline hydrogeologic investigation: Aquifer characterization

    Energy Technology Data Exchange (ETDEWEB)

    Strom, R.N.; Kaback, D.S.


    An investigation of the mineralogy and chemistry of the principal hydrogeologic units and the geochemistry of the water in the principal aquifers at Savannah River Site (SRS) was undertaken as part of the Baseline Hydrogeologic Investigation. This investigation was conducted to provide background data for future site studies and reports and to provide a site-wide interpretation of the geology and geochemistry of the Coastal Plain Hydrostratigraphic province. Ground water samples were analyzed for major cations and anions, minor and trace elements, gross alpha and beta, tritium, stable isotopes of hydrogen, oxygen, and carbon, and carbon-14. Sediments from the well borings were analyzed for mineralogy and major and minor elements.

  16. Biochemical adaptation to ocean acidification. (United States)

    Stillman, Jonathon H; Paganini, Adam W


    The change in oceanic carbonate chemistry due to increased atmospheric PCO2  has caused pH to decline in marine surface waters, a phenomenon known as ocean acidification (OA). The effects of OA on organisms have been shown to be widespread among diverse taxa from a wide range of habitats. The majority of studies of organismal response to OA are in short-term exposures to future levels of PCO2 . From such studies, much information has been gathered on plastic responses organisms may make in the future that are beneficial or harmful to fitness. Relatively few studies have examined whether organisms can adapt to negative-fitness consequences of plastic responses to OA. We outline major approaches that have been used to study the adaptive potential for organisms to OA, which include comparative studies and experimental evolution. Organisms that inhabit a range of pH environments (e.g. pH gradients at volcanic CO2 seeps or in upwelling zones) have great potential for studies that identify adaptive shifts that have occurred through evolution. Comparative studies have advanced our understanding of adaptation to OA by linking whole-organism responses with cellular mechanisms. Such optimization of function provides a link between genetic variation and adaptive evolution in tuning optimal function of rate-limiting cellular processes in different pH conditions. For example, in experimental evolution studies of organisms with short generation times (e.g. phytoplankton), hundreds of generations of growth under future conditions has resulted in fixed differences in gene expression related to acid-base regulation. However, biochemical mechanisms for adaptive responses to OA have yet to be fully characterized, and are likely to be more complex than simply changes in gene expression or protein modification. Finally, we present a hypothesis regarding an unexplored area for biochemical adaptation to ocean acidification. In this hypothesis, proteins and membranes exposed to the

  17. 瘤胃保护胆碱对围产期高产奶牛产奶性能和血液生化指标的影响%Effect of Rumen-protected Choline on Milk Performances and Blood Biochemical Parameters of High Productive Periparturient Dairy Cows

    Institute of Scientific and Technical Information of China (English)

    和立文; 孟庆翔; 王新宇; 刘辉; 李辉; 任丽萍


    This experiment was conducted to study the effect of rumen-protected choline (RPC) supplementation on milk performances and blood biochemical parameters of high productive periparturient dairy cows.Forty-four healthy Holstein cows with similar parity,expected calving date and milk yield were blocked into 22 pairs and then randomly allocated to either one of 2 treatments.Both groups received the same basal diet.The treatment group was supplemented with 20 g/d rumen-protected choline per cow.Treatments were applied from 2 wk before until 4 wk after calving,during which milk performances and blood biochemical parameters were investigated,respectively.The results showed that the average daily milk yield of the fourth week after calving increased by 2.31% (P>0.05).Lactose percentage decreased by 3.75% (P<0.05) and milk fat percentage,milk protein percentage,(milk fat)/(milk protein) and dry matter percentage increased by 11.11%,5.56%,6.38% and 2.94% without significant difference (P>0.05),respectively.There were no significant difference (P>0.05) on the content of both plasma triglyceride (TG) and β-hydroxybutyric acid (BHBA) between treatments on the time-point of calving,day 7 poscalving and day 14 postcalving,but the contents of plasma TG and BHBA of treatment group were apparently lower than the control.These results indicated that under the condition of this experiment RPC supplementation for high productive periparturient dairy cows could increase milk yield,improve milk quality and fat metabolism,relieve negative energy balance and reduce the risk of fat deposition in the liver.%为探究补充瘤胃保护胆碱(rumen-protected choline,RPC)对围产期高产奶牛产奶性能和血液生化指标的影响,试验选取44头健康的荷斯坦高产奶牛,按胎次、预产期、上一胎次产奶量等基本一致原则分为试验组和对照组,每组22头,饲喂相同基础日粮,试验组牛群补充20g/(d·头)的RPC.试验期内(产前14

  18. The Research of Blood Salt Levels and CSF Biochemical in Viral Enteritis Merger Combined with Convulsions%病毒性肠炎合并惊厥的血盐水平及脑脊液生化检查研究

    Institute of Scientific and Technical Information of China (English)



    目的:探讨病毒性肠炎合并惊厥的血盐水平及脑脊液病毒生化检查结果。方法:回顾性分析本院2010年1月-2014年10月收治的病毒性肠炎合并惊厥患儿69例的临床资料,将惊厥可按照发作频率分为频繁性(>3次)惊厥组(22例)与非频繁性(<3次)惊厥组(47例),按性质分为良性惊厥(52例)与非良性惊厥(17例),比较频繁性惊厥与非频繁性惊厥、良性惊厥与非良性惊厥患儿的血盐水平及脑脊液病毒生化检查结果。结果:惊厥频繁患儿的低血钠、低血钾、低血钙、低血镁及脑脊液生化检查病毒阳性比例均显著高于惊厥非频繁患儿(P<0.05);良性惊厥患儿体温、惊厥发作次数、惊厥持续时间及大便轮状病毒检查阳性率均显著低于非良性惊厥患儿(P<0.05)。结论:病毒性肠炎合并惊厥患儿中频繁性惊厥伴有低血钠、低血钾、低血钙、低血镁症状及脑脊液病毒感染,良性惊厥患儿的体温、惊厥发作次数、惊厥持续时间及大便轮状病毒检查阳性率均要低于非良性惊厥。%Objective:To explore the blood salt levels and CSF biochemical in Viral enteritis combined with convulsions. Method:The clinical date of 69 children with viral enteritis merger and convulsions in our hospital from January 2010 to October 2014 were retrospectively analysed.They were divided into frequent seizures(≥3 times) group(22 patients) and non-frequent (<3 times) seizures(47 cases) group according to the seizures frequency,according to the nature they were divided into benign convulsions (52 patients) and non-benign convulsions (17 patients).Salt levels in blood and viral biochemical test results of cerebrospinal fluid were compared between Frequent seizures and non-frequent seizures,Benign convulsions and Non-benign convulsions.Result:Frequent seizures group were significantly higher than the non-frequent seizures group(P<0.05) and

  19. Study of Some Hematological and Biochemical Parameters of Rainbow Trout (Oncorhynchus mykiss Fry

    Directory of Open Access Journals (Sweden)

    Salman Rauoof CHALKOO


    Full Text Available This study was done to investigate of some hematological and biochemical factors of rainbow trout (Oncorhynchus mykiss fry in Kashmir. About 50 pools of blood samples from diseased fry were collected within 30 months from November 2008 till March 2011 from three hatchery farms. In addition 30 pools of blood samples as control group were collected randomly from same farms. Each blood samples were examined for whole blood examination and blood enzymes measurement. It consist of total leukocytes (WBC and erythrocytes counts (RBC, hemoglobin (Hb content, hematocrits (PCV, leukocytes differential count and blood indices such as MCV, MCH and MCHC. Also blood serums were analysed for total protein (TP and blood enzymes. All the calculations were made using the SPSS© and t-test statistical method. In hematological findings nine parameters were revealed significant differences (P

  20. Baseline and benchmark model development for hotels (United States)

    Hooks, Edward T., Jr.

    The hotel industry currently faces rising energy costs and requires the tools to maximize energy efficiency. In order to achieve this goal a clear definition of the current methods used to measure and monitor energy consumption is made. Uncovering the limitations to the most common practiced analysis strategies and presenting methods that can potentially overcome those limitations is the main purpose. Techniques presented can be used for measurement and verification of energy efficiency plans and retrofits. Also, modern energy modeling tool are introduced to demonstrate how they can be utilized for benchmarking and baseline models. This will provide the ability to obtain energy saving recommendations and parametric analysis to explore energy savings potential. These same energy models can be used in design decisions for new construction. An energy model is created of a resort style hotel that over one million square feet and has over one thousand rooms. A simulation and detailed analysis is performed on a hotel room. The planning process for creating the model and acquiring data from the hotel room to calibrate and verify the simulation will be explained. An explanation as to how this type of modeling can potentially be beneficial for future baseline and benchmarking strategies for the hotel industry. Ultimately the conclusion will address some common obstacles the hotel industry has in reaching their full potential of energy efficiency and how these techniques can best serve them.

  1. Vegetation baseline report : Connacher great divide project

    Energy Technology Data Exchange (ETDEWEB)



    This baseline report supported an application by Connacher Oil and Gas Ltd. to the Alberta Energy and Utilities Board (EUB) and Alberta Environment (AENV) for the Great Divide Steam Assisted Gravity Drainage (SAGD) Project. The goal of the report was to document the distribution and occurrence of ecosite phases and wetland classes in the project footprint as well as to document the distribution of rare plants; rare plant communities: and intrusive species and old growth communities, including species of management concern. A methodology of the baseline report was presented, including details of mapping and field surveys. Six vegetation types in addition to the disturbed land unit were identified in the project footprint and associated buffer. It was noted that all vegetation types are common for the boreal forest natural regions. Several species of management concern were identified during the spring rare plant survey, including rare bryophytes and non-native or invasive species. Mitigation was identified through a slight shift of the footprint, transplant of appropriate bryophyte species and implementation of a weed management plan. It was noted that results of future surveys for rare plants will be submitted upon completion. It was concluded that the effects of the project on existing vegetation is expected to be low because of the small footprint, prior disturbance history, available mitigation measures and conservation and reclamation planning. 27 refs., 5 tabs., 4 figs.


    Directory of Open Access Journals (Sweden)

    A. R. Bilalova


    Full Text Available Purpose of the study. To carry out a comparative description of the clinical and biochemical parameters at patients with cirrhosis of an alcoholic genesis (CP-HGA and cirrhosis of mixed etiologies — CP-HGM (HСV+ alcohol.Materials and methods. The study involved 62 patients with cirrhosis of different etiologies, who carries out clinical, immunogenetic and biochemical studies.Results. Patients with the 3d genotype and low viral load were registered with cirrhosis of mixed etiologies (HСV+ alcohol. At the cirrhosis Class B for Child-Pugh basic data biochemical parameters were similar in patients with CP-HGA and CP-HGM, but ALT and AST activity, which are significantly higher than observed in patients with CP-HGM. At dismissal, ALT and GGT activities were detected significantly higher in patients with CP-HGM than the CP-HGA. At the cirrhosis Class C for Child-Pugh the baseline, reflecting cholestasis — is total bilirubin, GGT and alkaline phosphatase and were detected significantly higher in the CP-HGA, than with CP-HGM significantly reduced, and thore is no differences between the groups to be discharged from the hospital, in addition to the activity of GGT, which it remained significantly higher in the CP-HGA, than with CP-HGM. Cytolytic activity of enzymes (ALT, AST during the entire period of the disease was observed significantly higher normal values and did not depend on the CP etiology.Conclusion decision. The maximum rate of change of the basic biochemical parameters is observed in patients with cirrhosis of mixed etiologies Class B for Child-Pugh and at the cirrhosis of an alcoholic genesis — in patients with cirrhosis Class C.

  3. The correlation between coronary flow reserve and clinical outcomes in patients with primary ;angioplasty for acute myocardial infarction with high baseline white blood cell count%基础白细胞计数增高的急性心肌梗死患者冠状动脉介入术后冠状动脉血流储备降低对远期预后的影响

    Institute of Scientific and Technical Information of China (English)

    郑振国; 周旭晨; 朱皓; 尹达; 张妍


    Objective Both abnormal coronary flow reserve (CFR) following percutaneous coronary intervention (PCI) and elevated white blood cell (WBC) count in acute myocardial infarction (AMI) are known as useful predictors for adverse outcomes. We evaluated the value of abnormal CFR for prediction of adverse outcomes in AMI patients with high baseline WBC count following primary PCI. Methods We studied 72 consecutive patients (mean age 56±12, male 55) presenting with AMI who were admitted to our institution. Analyze of CFR was performed following PCI by intra-coronary Doppler wire. All enroll patents’ baseline WBC count was obtained at admission and WBC count≥10.4×109/L. The occurrence of major adverse cardiac events (MACE) were analyzed during clinical follow up (12.7±7.1 months). According to the best cutoff value of CFR for predicting MACE, the patients were divided into 2 groups:group 1 with CFR<1.4, group 2 with CFR≥1.4. Results Baseline CK and CK-MB in group 1 were significantly higher than in group 2[(4 109±407)U/L vs. (2 685±562)U/L, P<0.05; (290.8± 26.6)ng/ml vs. (255.7±65.6)ng/ml, P<0.05 respectively]; Percent of post PCI MBG grade3 were more often in group 2 than in group 1(25.9%vs. 38.9%, P<0.05). MACE rate were significantly higher in group 1 than in group 2(40.7%vs. 27.8%, P<0.05), the mortality rate was significantly higher in group 1 than in group 2(13.0% vs. 0, P<0.05). Conclusions Abnormal CFR following PCI with high baseline WBC count in patients with AMI were associated with a higher incidence of adverse long-term clinical outcomes. This observation may provide a potential explanation that links of microvascular dysfunction and vascular inflammation in patients with AMI.%目的:急性心肌梗死(AMI)患者在接受了冠状动脉介入术(PCI)后,即刻冠状动脉血流储备(CFR)的降低,以及AMI急性期基础血液中白细胞计数的增加均被视为AMI患者远期预后不良的预测因子。我们现将这

  4. Tightly coupled long baseline/ultra-short baseline integrated navigation system (United States)

    Batista, Pedro; Silvestre, Carlos; Oliveira, Paulo


    This paper proposes a novel integrated navigation filter based on a combined long baseline/ultra short baseline acoustic positioning system with application to underwater vehicles. With a tightly coupled structure, the position, linear velocity, attitude, and rate gyro bias are estimated, considering the full nonlinear system dynamics without resorting to any algebraic inversion or linearisation techniques. The resulting solution ensures convergence of the estimation error to zero for all initial conditions, exponentially fast. Finally, it is shown, under simulation environment, that the filter achieves very good performance in the presence of sensor noise.

  5. Biochemical structure of Calendula officinalis. (United States)

    Korakhashvili, A; Kacharava, T; Kiknavelidze, N


    Calendula officinalis is a well known medicinal herb. It is common knowledge that its medicinal properties are conditioned on biologically active complex substances of Carotin (Provitamin A), Stearin, Triterpiniod, Plavonoid, Kumarin, macro and micro compound elements. Because of constant need in raw material of Calendula officinalis, features of its ontogenetic development agro-biological qualities in various eco regions of Georgia were investigated. The data of biologically active compounds, biochemical structure and the maintenance both in flowers and in others parts of plant is presented; the pharmacological activity and importance in medicine was reviewed.

  6. Time course for the recovery of physical performance, blood hemoglobin, and ferritin content after blood donation

    DEFF Research Database (Denmark)

    Ziegler, Andreas K; Grand, Johannes; Stangerup, Ida;


    BACKGROUND: It is widely accepted that blood donation negatively affects endurance performance, but data on physical recovery after a standard blood donation are scarce. This study aimed to elucidate the temporary impact of blood donation on endurance performance, measured as peak oxygen uptake (VO......2peak ) and time trial (TT) performance. STUDY DESIGN AND METHODS: VO2peak , TT performance, blood, iron, and anthropometric variables were determined before (baseline) and 3, 7, 14, and 28 days after blood donation in 19 healthy men. RESULTS: VO2peak was reduced by 6.5% from 49.7 ± 2 m......L/kg/min at baseline to 46.3 ± 2 mL/kg/min on Day 3 (p blood donation. Blood hemoglobin (Hb) concentration declined 7.9% from 9.3 ± 0.11 mmol...

  7. Pentek concrete scabbling system: Baseline report

    Energy Technology Data Exchange (ETDEWEB)



    The Pentek scabbling technology was tested at Florida International University (FIU) and is being evaluated as a baseline technology. This report evaluates it for safety and health issues. It is a commercially available technology and has been used for various projects at locations throughout the country. The Pentek concrete scabbling system consisted of the MOOSE{reg_sign}, SQUIRREL{reg_sign}-I, and SQUIRREL{reg_sign}-III scabblers. The scabblers are designed to scarify concrete floors and slabs using cross-section, tungsten carbide tipped bits. The bits are designed to remove concrete in 318 inch increments. The bits are either 9-tooth or demolition type. The scabblers are used with a vacuum system designed to collect and filter the concrete dust and contamination that is removed from the surface. The safety and health evaluation during the human factors assessment focused on two main areas: noise and dust.

  8. The WITCH Model. Structure, Baseline, Solutions.

    Energy Technology Data Exchange (ETDEWEB)

    Bosetti, V.; Massetti, E.; Tavoni, M.


    WITCH - World Induced Technical Change Hybrid - is a regionally disaggregated hard link hybrid global model with a neoclassical optimal growth structure (top down) and an energy input detail (bottom up). The model endogenously accounts for technological change, both through learning curves affecting prices of new vintages of capital and through R and D investments. The model features the main economic and environmental policies in each world region as the outcome of a dynamic game. WITCH belongs to the class of Integrated Assessment Models as it possesses a climate module that feeds climate changes back into the economy. In this paper we provide a thorough discussion of the model structure and baseline projections. We report detailed information on the evolution of energy demand, technology and CO2 emissions. Finally, we explicitly quantifiy the role of free riding in determining the emissions scenarios. (auth)

  9. Octant degeneracy, CPV phase at Long Baseline $\

    CERN Document Server

    Bora, Kalpana; Dutta, Debajyoti


    In a recent work by two of us, we have studied, how CP violation discovery potential can be improved at long baseline neutrino experiments (LBNE/DUNE), by combining with its ND (near detector) and reactor experiments. In this work, we discuss how this study can be further analysed to resolve entanglement of the quadrant of CPV phase and Octant of atmospheric mixing angle {\\theta}23, at LBNEs. The study is done for both NH (Normal hierarchy) and IH (Inverted hierarchy). We further show how leptogenesis can enhance this effect of resolving this entanglement. A detailed analytic and numerical study of baryogenesis through leptogenesis is performed in this framework in a model independent way. We then compare our result of the baryon to photon ratio with the the current observational data of the baryon asymmetry.

  10. Intensity interferometry: Optical imaging with kilometer baselines

    CERN Document Server

    Dravins, Dainis


    Optical imaging with microarcsecond resolution will reveal details across and outside stellar surfaces but requires kilometer-scale interferometers, challenging to realize either on the ground or in space. Intensity interferometry, electronically connecting independent telescopes, has a noise budget that relates to the electronic time resolution, circumventing issues of atmospheric turbulence. Extents up to a few km are becoming realistic with arrays of optical air Cherenkov telescopes (primarily erected for gamma-ray studies), enabling an optical equivalent of radio interferometer arrays. Pioneered by Hanbury Brown and Twiss, digital versions of the technique have now been demonstrated, reconstructing diffraction-limited images from laboratory measurements over hundreds of optical baselines. This review outlines the method from its beginnings, describes current experiments, and sketches prospects for future observations.

  11. The OPERA long baseline neutrino oscillation experiment (United States)

    Wilquet, G.


    OPERA is a long baseline neutrino oscillation experiment designed to observe the appearance of vτ in a pure vμ beam in the parameter space indicated by the atmospheric neutrinos oscillation signal. The detector is situated in the underground LNGS laboratory under 3 800 water meter equivalent at a distance of 730 km from CERN where the CNGS neutrino beam to which it is exposed originates. It consists of two identical 0.68 kilotons lead/nuclear emulsion targets, each instrumented with a tracking device and complemented by a muon spectrometer. The concept and the status of the detector are described and the first results obtained with cosmic rays and during two weeks of beam commissioning in 2006 are reported.

  12. In-Space Manufacturing Baseline Property Development (United States)

    Stockman, Tom; Schneider, Judith; Prater, Tracie; Bean, Quincy; Werkheiser, Nicki


    The In-Space Manufacturing (ISM) project at NASA Marshall Space Flight Center currently operates a 3D FDM (fused deposition modeling) printer onboard the International Space Station. In order to enable utilization of this capability by designer, the project needs to establish characteristic material properties for materials produced using the process. This is difficult for additive manufacturing since standards and specifications do not yet exist for these technologies. Due to availability of crew time, there are limitations to the sample size which in turn limits the application of the traditional design allowables approaches to develop a materials property database for designers. In this study, various approaches to development of material databases were evaluated for use by designers of space systems who wish to leverage in-space manufacturing capabilities. This study focuses on alternative statistical techniques for baseline property development to support in-space manufacturing.

  13. Steganography Based on Baseline Sequential JPEG Compression

    Institute of Scientific and Technical Information of China (English)


    Information hiding in Joint Photographic Experts Group (JPEG) compressed images are investigated in this paper. Quantization is the source of information loss in JPEG compression process. Therefore, information hidden in images is probably destroyed by JPEG compression. This paper presents an algorithm to reliably embed information into the JPEG bit streams in the process of JPEG encoding. Information extraction is performed in the process of JPEG decoding. The basic idea of our algorithm is to modify the quantized direct current (DC) coefficients and non-zero alternating current (AC) coefficients to represent one bit information (0 or 1). Experimental results on gray images using baseline sequential JPEG encoding show that the cover images (images without secret information) and the stego-images (images with secret information) are perceptually indiscernible.

  14. Radio sources - Very, Very Long Baseline Interferometry (United States)

    Roberts, D. H.


    With resolution of a thousandth of an arcsecond, the radio technique of Very Long Baseline Interferometry (VLBI) provides astronomers with their highest-resolution view of the universe. Data taken with widely-separated antennas are combined, with the help of atomic clocks, to form a Michelson interferometer whose size may be as great as the earth's diameter. Extraordinary phenomena, from the birth of stars as signaled by the brilliant flashes of powerful interstellar masers to the 'faster-than-light' expansion of the cores of distant quasars, are being explored with this technique. However, earth-bound VLBI suffers from several restrictions due to the location of the component antennas at fixed places on the earth's surface. The use of one or more antennas in space in concert with ground-based equipment will greatly expand the technical and scientific capabilities of VLBI, leading to a more complete and even higher resolution view of cosmic phenomena.

  15. Biochemical responses to fibropapilloma and captivity in the green turtle. (United States)

    Swimmer, J Y


    Blood biochemical parameters were compared for green turtles (Chelonia mydas) with and without green turtle fibropapillomatosis (GTFP) from both captive and wild populations in Hawaii (USA) and from a captive population from California (USA), during the period between 1994 and 1996. Statistical analysis did not detect an influence of disease in any of the blood parameters for free-ranging turtles; however, captive turtles in Hawaii with GTFP had significantly higher levels of alkaline phosphatase and significantly lower levels of lactate compared to non-tumored captive turtles. Multivariate analysis found that biochemical profiles could be used to accurately predict if turtles were healthy or afflicted with GTFP. Discriminant function analysis correctly classified turtles as being with or without GTFP in 89% of cases, suggesting that diseased animals had a distinct signature of plasma biochemistries. Measurements of blood parameters identified numerous differences between captive and wild green turtles in Hawaii. Levels of corticosterone, lactate, triglyceride, glucose, and calcium were significantly higher in wild green turtles as compared to captive turtles, while uric acid levels were significantly lower in wild turtles as compared to captive turtles. Additionally, turtles from Sea World of California (San Diego, California, USA), which had been in captivity the longest, had higher levels of alanine aminotransferase and triglycerides as compared to nearly all other groups. Differences in diet, sampling methods, environmental conditions, and turtle size, help to interpret these results.

  16. Biological baseline data Youngs Bay, Oregon, 1974

    Energy Technology Data Exchange (ETDEWEB)

    McMechan, K.J. (ed.); Higley, D.L.; Holton, R.L.


    This report presents biological baseline information gathered during the research project, Physical, Chemical and Biological Studies on Youngs Bay.'' Youngs Bay is a shallow embayment located on the south shore of the Columbia River, near Astoria, Oregon. Research on Youngs Bay was motivated by the proposed construction by Alumax Pacific Aluminum Corporation of an aluminum reduction plant at Warrenton, Oregon. The research was designed to provide biological baseline information on Youngs Bay in anticipation of potential harmful effects from plant effluents. The information collected concerns the kinds of animals found in the Youngs Bay area, and their distribution and seasonal patterns of abundance. In addition, information was collected on the feeding habits of selected fish species, and on the life history and behavioral characteristics of the most abundant benthic amphipod, Corophium salmonis. Sampling was conducted at approximately three-week intervals, using commonly accepted methods of animal collection. Relatively few stations were sampled for fish, because of the need to standardize conditions of capture. Data on fish capture are reported in terms of catch-per-unit effort by a particular sampling gear at a specific station. Methods used in sampling invertebrates were generally more quantitative, and allowed sampling at a greater variety of places, as well as a valid basis for the computation of densities. Checklists of invertebrate species and fish species were developed from these samples, and are referred to throughout the report. The invertebrate checklist is more specific taxonomically than are tables reporting invertebrate densities. This is because the methods employed in identification were more precise than those used in counts. 9 refs., 27 figs., 25 tabs.

  17. Baseline hematology and clinical chemistry results from captive-raised trumpeter swans (United States)

    Olsen, G.H.; Rininger, D.L.; Ets, M.K.; Sladen, William J. L.; Rees, Eileen C.; Earnst, Susan L.; Coulson, John C.


    Results from hematology and clinical chemistry tests are presented for healthy captive-raised Trumpeter Swans (Cygnus buccinator) to help establish baseline data. Blood samples were obtained from 14 cygnets between the ages of three to four and seven to eight months that were the subjects of a study to teach migration routes to swans. Males and females differed significantly in asparatate aminotransferase, alanine aminotransferase and total protein. Age categories differed significantly in hematocrit, white blood cell counts, alkaline phosphatase, aspar-rate aminotransferase, glucose, cholesterol and uric acid. There were no significant differences among age categories in values of alanine aminotransferase, calcium, triglycerides and total protein.

  18. Blood Clots (United States)

    ... or prevent blood clots from dissolving properly. Risk factors for excessive blood clotting include Certain genetic disorders Atherosclerosis Diabetes Atrial fibrillation Overweight, obesity, and metabolic syndrome Some medicines Smoking deep vein ...

  19. Blood Transfusion (United States)

    ... to their work or home. The availability of plastic bags that can have one or more satellite bags ... in preparing the donated blood. The use of plastic bags allows the blood center to make a variety ...

  20. Biochemical aspects of Huntington's chorea. (United States)

    Caraceni, T; Calderini, G; Consolazione, A; Riva, E; Algeri, S; Girotti, F; Spreafico, R; Branciforti, A; Dall'olio, A; Morselli, P L


    Fifteen patients affected by Huntington's chorea were divided into two groups, 'slow' and 'fast', according to IQ scores on the Wechsler-Bellevue scale, and scores on some motor performance tests. A possible correlation was looked for between some biochemical data (cerebrospinal fluid (CSF), homovanillic acid (HVA), and 5-hydroxyindolacetic acid (5HIAA) levels, plasma dopamine-beta-hydroxylase (DBH), dopamine (DA) uptake by platelets), and clinical data (duration of illness, severity of symptoms, age of patients, IQ scores, 'slow' and 'fast' groups). The CSF, HVA, and 5HIAA levels were found to be significantly lowered in comparison with normal controls. DBH activity and DA uptake by platelets did not differ significantly from normal subjects. Treatment with haloperidol in all patients and with dipropylacetic acid in three patients did not appear to modify the CSF, HVA, and 5HIAA concentrations, the plasma DBH activity, or the DA uptake. There were no significant differences in the CSF, HVA, and 5HIAA contents between the two groups of patients, and there was no correlation between biochemical data and clinical features. PMID:143508

  1. Biochemical abnormalities in Pearson syndrome. (United States)

    Crippa, Beatrice Letizia; Leon, Eyby; Calhoun, Amy; Lowichik, Amy; Pasquali, Marzia; Longo, Nicola


    Pearson marrow-pancreas syndrome is a multisystem mitochondrial disorder characterized by bone marrow failure and pancreatic insufficiency. Children who survive the severe bone marrow dysfunction in childhood develop Kearns-Sayre syndrome later in life. Here we report on four new cases with this condition and define their biochemical abnormalities. Three out of four patients presented with failure to thrive, with most of them having normal development and head size. All patients had evidence of bone marrow involvement that spontaneously improved in three out of four patients. Unique findings in our patients were acute pancreatitis (one out of four), renal Fanconi syndrome (present in all patients, but symptomatic only in one), and an unusual organic aciduria with 3-hydroxyisobutyric aciduria in one patient. Biochemical analysis indicated low levels of plasma citrulline and arginine, despite low-normal ammonia levels. Regression analysis indicated a significant correlation between each intermediate of the urea cycle and the next, except between ornithine and citrulline. This suggested that the reaction catalyzed by ornithine transcarbamylase (that converts ornithine to citrulline) might not be very efficient in patients with Pearson syndrome. In view of low-normal ammonia levels, we hypothesize that ammonia and carbamylphosphate could be diverted from the urea cycle to the synthesis of nucleotides in patients with Pearson syndrome and possibly other mitochondrial disorders.

  2. Vector Encoding in Biochemical Networks (United States)

    Potter, Garrett; Sun, Bo

    Encoding of environmental cues via biochemical signaling pathways is of vital importance in the transmission of information for cells in a network. The current literature assumes a single cell state is used to encode information, however, recent research suggests the optimal strategy utilizes a vector of cell states sampled at various time points. To elucidate the optimal sampling strategy for vector encoding, we take an information theoretic approach and determine the mutual information of the calcium signaling dynamics obtained from fibroblast cells perturbed with different concentrations of ATP. Specifically, we analyze the sampling strategies under the cases of fixed and non-fixed vector dimension as well as the efficiency of these strategies. Our results show that sampling with greater frequency is optimal in the case of non-fixed vector dimension but that, in general, a lower sampling frequency is best from both a fixed vector dimension and efficiency standpoint. Further, we find the use of a simple modified Ornstein-Uhlenbeck process as a model qualitatively captures many of our experimental results suggesting that sampling in biochemical networks is based on a few basic components.

  3. Digital Offshore Cadastre (DOC) - Pacific83 - Baseline Tangent Lines (United States)

    Bureau of Ocean Energy Management, Department of the Interior — This data set contains baseline tangent lines and bay closing lines in ESRI Arc/Info export and Arc/View shape file formats for the BOEM Pacific Region. Baseline...

  4. Toxicity of Moringa oleifera seed extract on some hematological and biochemical profiles in a freshwater fish, Cyprinus carpio. (United States)

    Kavitha, Chokkalingam; Ramesh, Mathan; Kumaran, Satyanarayanan Senthil; Lakshmi, Srinivasan Audhi


    The study was carried out to investigate the acute and sublethal toxicity of Moringa oleifera seed extract on hematological and biochemical variables of a freshwater fish Cyprinus carpio under laboratory conditions. The 96 h LC50 value of M. oleifera seed extract to the fish C. carpio was estimated by probit analysis method and was found to be 124.0 mg/L (with 95% confidence limits). For sublethal studies a non lethal dose of 1/10th of 96 h LC50 value (12.40 mg/L) was taken. During acute treatment (96 h), hematological variables like red blood cell count (RBC), hemoglobin (Hb), hematocrit (Hct), and mean corpuscular hemoglobin concentration (MCHC) were significantly (Pseed extract. However a significant (Pseed extract while a significant (Pseed extract exposed fish, whereas plasma protein level declined. However, a biphasic trend was noticed in Hb, Hct, RBC and MCHC levels. This study may provide baseline information about the toxicity of M. oleifera seed extract to C. carpio and to establish safer limit in water purification.

  5. The Effect of Echinacea Compound on Performance and Blood Biochemical Indexes of Laying Hens under High Temperature%紫锥菊复方对高温条件下蛋鸡生产性能及血液生化指标的影响

    Institute of Scientific and Technical Information of China (English)

    代雪立; 石达友; 莫桂芬; 单奇; 郭世宁


    In order to study the effect of Echinacea compound on laying hens' production performance and blood biochemical indexes under high temperature, 250 aged 60-week Highland Brown laying hens were randomly divided into five groups: 0. 5% dose of Echinacea compound group, 1% dose of Echinacea compound group, 1.5% dose of Echinacea compound group, 0. 04% dose of vitamin C group and control group. The results showed that the 1% dose of Echinacea compound and 1. 5% dose of Echinacea compound could significantly enhance the feed intake of chickens,the egg production rate(P<0. 05), lower the feed egg ratio(P<0. 05) .under high temperature; the vitamin C's effect of improving the feed intake of chickens, the egg production rate, the feed egg ratio of laying hens was not significant under high temperature, but it could reduce the soft-broken egg rate and the chickens died number. Compared with the control group, the different concentrations of Echinacea compound and vitamin C could increase the serum's GLU, TP, ALB content and ALP activity, reduce the serum's TG, CHO concentration and AST, ALT, CK activity.%为研究紫锥菊复方对高温条件下蛋鸡生产性能和血液生化指标的影响,选用60周龄海兰褐蛋鸡250只,随机分为5组,试验第1~4组日粮中分别添加0.5%、1%、1.5%的紫锥菊复方和0.04%维生索C,第5组为空白对照组.试验结果表明,1%紫锥菊复方和1.5%紫锥菊复方能够显著提高高温条件下蛋鸡的采食量、产蛋率(P<0.05),降低料蛋比(P<0.05);维生素C对于高温条件下蛋鸡采食量、产蛋率、料蛋比的影响差异不显著(P>0.05),但可降低蛋鸡软破蛋比率和鸡群死淘数;与对照组相比,不同浓度的紫锥菊复方和维生素C均可明显提高血清GLU、TP、ALB水平和ALP活性,降低血清TG、CHO浓度和ALT、CK活性.

  6. 甜菜碱对奥尼罗非鱼生长、体脂含量及血清生化指标的影响%Effects of dietary Betaine on growth, fat content and biochemical indexs in blood of tilapia(Oreochromis niloticus × Oreochrmis aureus)

    Institute of Scientific and Technical Information of China (English)

    朱定贵; 于丹; 陈涛; 战歌; 黄凯; 麻艳群; 杨思华


    750 juvenile tilapia( Oreochromis niloticus × Oreochrmis aureu.s ) were randomly allotted to five groups, and were fed diets supplemented with different level Betaine (0、0.15% 、0.30% 、0.45% and 0.60% ) for 65 d to investigate the effects of Betaine on growth, fat content and biochemical indexs of blood of tilapia.The results showed that tilapia' WGR were 19.52%、 16.63% and 21.38% higher than that of control group ( P < 0.05 ) respectively when tilapia feed were added with 0.30% - 0.60% Betaine; FRC reduced by 25.55% 、20.44 % and 29.93% ( P < 0.05 ) respectively; the HSI reduced by 9.29% 、38.57% and 29.29% ( P < 0.05 ); the contents of TG in Tilapia' s plasma reduced by 66.28% 、55.81% and 80.23% (P <0.05) respectively; the activities of AMY in Tilapia' s plasma reduced by 88.78% 、37.59% and 62.41% ( P < 0.05 ); the contents of ALB and TP in Tilapia' s plasma were improved, the contents of CHOL in Tilapia' s plasma reduced; the activities of LDH、 ALT and AST in Tilapia' s plasma reduced respectively.Therefore, under this research condition, it is suggested that the supplement of Betaine in diets of juvenile tilapia was the best at 0.45% ~ 0.60%.%取奥尼罗非鱼(Oreochromis niloticus×Oreochrmis aureus)750尾,随机分为5组,分别饲喂甜菜碱(Betaine)添加量为0、0.15%、0.30%、0.45%和0.60%的饲料,饲养65 d,以研究甜菜碱对奥尼罗非鱼生长、体脂含量及血清生化指标的影响.结果表明:当甜菜碱添加量为0.30%~0.60%时,罗非鱼的增重率较对照组分别提高19.52%、16.63%和21.38%;饲料系数分别降低25.55%、20.44%和29.93%;肝体比降低9.29%、38.57%和29.29%;血清中甘油三酯含量较对照组分别降低66.28%、55.81%和80.23%;淀粉酶活性降低88.78%、37.59%和62.41%;白蛋白和总蛋白含量升高,胆固醇含量显著降低;谷丙转氨酶、谷草转氨酶、乳酸脱氧酶活性均有所降低.在本研究条件下,

  7. Effects of Lipase Enzyme on Hrowth Performance, Apparent Metabolizable Energy of Nutrients, Blood Biochemical Parameters and Abdominal Fat Fate of Broilers%脂肪酶对肉鸡生长性能、表观代谢能、血液生化指标及腹脂率的影响

    Institute of Scientific and Technical Information of China (English)

    李芳林; 石宝明; 单安山; 时本利; 王剑英


    试验研究了在玉米一豆粕型日粮中添加脂肪酶对商品肉仔鸡生长性能、表观代谢能、血液生化指标和腹脂率等的影响。试验选用1日龄健康爱维因肉仔鸡240只,随机分为4个处理组,每个处理4个重复,每个重复15只鸡。试验期8用。整个饲养期分为3个阶段,0~3周为生长前期,4-6周为生长中期,7~8周为生长后期。结果表明,日粮中添加脂肪酶可显著提高肉鸡的日增重(P〈0.05),降低料肉比(P〈0.05),提高表观代谢能(P〈0.05),降低血液中甘油三酯、总胆固醇、低密度脂蛋白(P〈0.05),提高高密度脂蛋白含量(P〈0.05),降低腹脂率(P〈0.05).%This article studied the effect of lipase enzyme on growth performance, apparent metabolizable energy of nutrients, blood biochemical parameters amt abdominal fat rate in corn-soybean meal-based diets of broilers. Experiment chose 240 one-day health AA broilers, randomly allocated to 4 groups with 4 replicates per group and 15 broilers per replicate with 8 weeks trial period. The feeding period was divided into three stages: 0-3 week for the early growth stage, 4-6 week for the medium growth stage and 7-8 week for the late growth stage. Experiment showed that lipase enzyme can improve ADG(P〈0.05), reduce F/G(P〈0.05) and improve apparent metabolizable energy(P〈0.05) of nutrients of broilers, when it reduced triglyceride, total cholesterol, low-density lipoproteinand(P〈 0.05) and improved high-density lipoprotein(P〈0.05), it reduced abdominal fat rate of broilers(P〈0.05).

  8. Net haemoglobin increase from reinfusion of refrigerated vs. frozen red blood cells after autologous blood transfusions

    DEFF Research Database (Denmark)

    Ashenden, M; Mørkeberg, Jakob Sehested


    objective was to examine which storage procedure yielded the largest increase in circulating haemoglobin after reinfusion compared to baseline. MATERIALS AND METHODS  Equal volumes of blood from 15 men were withdrawn and stored either frozen or refrigerated as packed red blood cells. Serial measures...... freezing. Nevertheless, frozen storage allowed haemoglobin to fully recover before reinfusion, while the haemoglobin was 10% lower in the refrigerated group compared with baseline. After reinfusion, the haemoglobin levels were 11·5% higher than the baseline values in the group reinfused with frozen blood......, while for the refrigerated group, haemoglobin levels were only 5·2% higher than baseline. CONCLUSION  The relatively larger recovery from anaemia in the frozen group during storage more than compensated for the larger loss of haemoglobin during freezing and resulted in a larger net gain in haemoglobin...

  9. The effect of short-baseline neutrino oscillations on LBNE

    Energy Technology Data Exchange (ETDEWEB)

    Louis, William C. [Physics Division, Los Alamos National Laboratory, Los Alamos, NM 87545 (United States)


    Short-baseline neutrino oscillations can have a relatively big effect on long-baseline oscillations, due to the cross terms that arise from multiple mass scales. The existing short-baseline anomalies suggest that short-baseline oscillations can affect the ν{sub μ} → ν{sub e} appearance probabilities by up to 20-40%, depending on the values of the CP-violating parameters.

  10. The effect of short-baseline neutrino oscillations on LBNE (United States)

    Louis, William C.


    Short-baseline neutrino oscillations can have a relatively big effect on long-baseline oscillations, due to the cross terms that arise from multiple mass scales. The existing short-baseline anomalies suggest that short-baseline oscillations can affect the νμ → νe appearance probabilities by up to 20-40%, depending on the values of the CP-violating parameters.

  11. Biochemical and haematological profile of pheasant hens during the laying period. (United States)

    Schumann, J; Bedanova, I; Voslarova, E; Hrabcakova, P; Chloupek, J; Pistekova, V


    The present paper provides new experimental data on the biochemical and haematological profile of blood in pheasant hens, and points out the changes in both biochemical and haematological parameters that occur during the laying period. Significant effects of egg laying on both the biochemical and the haematological blood parameters of pheasant hens were found. Biochemical analyses revealed a significant increase in the metabolites cholesterol, uric acid, lactate, the enzyme aspartate aminotransferase (AST) and the minerals calcium and phosphorous, as well as a significant decrease in total protein, albumin and glucose in the course of the laying period. Haematological analyses revealed a significant increase in the count of leukocytes, lymphocytes, eosinophils, basophils and monocytes due to egg laying. In addition, the erythrocyte count and haemoglobin content significantly decreased in the middle of the laying period and then rebounded at the end of the laying period. The haematocrit content gradually decreased till the end of the laying period. All together, the results of this study underline the impact of the reproduction status of pheasant hens on basic blood parameters. The biochemical and haematological values presented in this study may be of help in assessing disease conditions in laying pheasant hens.

  12. Diurnal changes of biochemical metabolic markers in healthy young males

    DEFF Research Database (Denmark)

    Sennels, Henriette P; Jørgensen, Henrik L; Fahrenkrug, Jan


    .06 mmol/L) did not show significant oscillations. CONCLUSIONS: When diagnosing and monitoring metabolic disorders compensation for the 24-h variation of the biochemical metabolic markers is needed especially C-peptide, triglyceride and glucose. Furthermore, the stable HbA1c level through 24 h makes......BACKGROUND: To examine whether time of the day has an effect on the circulating levels of metabolism parameters. METHODS: Venous blood samples were obtained under standardized conditions from 24 healthy young men every third hour through 24 hours. The metabolic markers and melatonin were examined...

  13. Baseline glucocorticoids are drivers of body mass gain in a diving seabird (United States)

    Hennin, Holly; Berlin, Alicia; Love, Oliver P.


    Life-history trade-offs are influenced by variation in individual state, with individuals in better condition often completing life-history stages with greater success. Although resource accrual significantly impacts key life-history decisions such as the timing of reproduction, little is known about the underlying mechanisms driving resource accumulation. Baseline corticosterone (CORT, the primary avian glucocorticoid) mediates daily and seasonal energetics, responds to changes in food availability, and has been linked to foraging behavior, making it a strong potential driver of individual variation in resource accrual and deposition. Working with a captive colony of white-winged scoters (Melanitta fusca deglandi), we aimed to causally determine whether variation in baseline CORT drives individual body mass gains mediated through fattening rate (plasma triglycerides corrected for body mass). We implanted individuals with each of three treatment pellets to elevate CORT within a baseline range in a randomized order: control, low dose of CORT, high dose of CORT, then blood sampled and recorded body mass over a two-week period to track changes in baseline CORT, body mass, and fattening rates. The high CORT treatment significantly elevated levels of plasma hormone for a short period of time within the biologically relevant, baseline range for this species, but importantly did not inhibit the function of the HPA (hypothalamic–pituitary–adrenal) axis. Furthermore, an elevation in baseline CORT resulted in a consistent increase in body mass throughout the trial period compared to controls. This is some of the first empirical evidence demonstrating that elevations of baseline CORT within a biologically relevant range have a causal, direct, and positive influence on changes in body mass.

  14. Iron deficiency in blood donors

    Directory of Open Access Journals (Sweden)

    Armando Cortés


    Full Text Available Context: Blood donation results in a substantial loss of iron (200 to 250 mg at each bleeding procedure (425 to 475 ml and subsequent mobilization of iron from body stores. Recent reports have shown that body iron reserves generally are small and iron depletion is more frequent in blood donors than in non-donors. Objective: The aim of this study was to evaluate the frequency of iron deficiency in blood donors and to establish the frequency of iron deficiency in blood donors according to sex, whether they were first-time or multi-time donors. Design: From march 20 to April 5, 2004, three hundred potential blood donors from Hemocentro del Café y Tolima Grande were studied. Diagnostic tests: Using a combination of biochemical measurements of iron status: serum ferritin (RIA, ANNAR and the hemoglobin pre and post-donation (HEMOCUE Vital technology medical . Results: The frequency of iron deficiency in potential blood donors was 5%, and blood donors accepted was 5.1%; in blood donors rejected for low hemoglobin the frequency of iron deficiency was 3.7% and accepted blood donors was 1.7% in male and 12.6% in female. The frequency of iron deficiency was higher in multi-time blood donors than in first-time blood donors, but not stadistic significative. Increase nivel accepted hemoglobina in 1 g/dl no incidence in male; in female increase of 0.5 g/dl low in 25% blood donors accepted with iron deficiency, but increased rejected innecesary in 16.6% and increased is 1 g/dl low blood donors female accepted in 58% (7/12, but increased the rejected innecesary in 35.6%. Conclusions: We conclude that blood donation not is a important factor for iron deficiency in blood donors. The high frequency of blood donors with iron deficiency found in this study suggests a need for a more accurate laboratory trial, as hemoglobin or hematocrit measurement alone is not sufficient for detecting and excluding blood donors with iron deficiency without anemia, and ajustes hacia

  15. 10 CFR 850.20 - Baseline beryllium inventory. (United States)


    ... 10 Energy 4 2010-01-01 2010-01-01 false Baseline beryllium inventory. 850.20 Section 850.20 Energy... Baseline beryllium inventory. (a) The responsible employer must develop a baseline inventory of the... inventory, the responsible employer must: (1) Review current and historical records; (2) Interview...

  16. Blood Facts and Statistics (United States)

    ... Facts and Statistics Printable Version Blood Facts and Statistics Facts about blood needs Facts about the blood ... to Top Learn About Blood Blood Facts and Statistics Blood Components Whole Blood and Red Blood Cells ...

  17. Nonlinear Biochemical Signal Processing via Noise Propagation


    Kim, Kyung Hyuk; Qian, Hong; Sauro, Herbert M.


    Single-cell studies often show significant phenotypic variability due to the stochastic nature of intra-cellular biochemical reactions. When the numbers of molecules, e.g., transcription factors and regulatory enzymes, are in low abundance, fluctuations in biochemical activities become significant and such "noise" can propagate through regulatory cascades in terms of biochemical reaction networks. Here we develop an intuitive, yet fully quantitative method for analyzing how noise affects cell...

  18. Cord Blood

    Directory of Open Access Journals (Sweden)

    Saeed Abroun


    Full Text Available   Stem cells are naïve or master cells. This means they can transform into special 200 cell types as needed by body, and each of these cells has just one function. Stem cells are found in many parts of the human body, although some sources have richer concentrations than others. Some excellent sources of stem cells, such as bone marrow, peripheral blood, cord blood, other tissue stem cells and human embryos, which last one are controversial and their use can be illegal in some countries. Cord blood is a sample of blood taken from a newborn baby's umbilical cord. It is a rich source of stem cells, umbilical cord blood and tissue are collected from material that normally has no use following a child’s birth. Umbilical cord blood and tissue cells are rich sources of stem cells, which have been used in the treatment of over 80 diseases including leukemia, lymphoma and anemia as bone marrow stem cell potency.  The most common disease category has been leukemia. The next largest group is inherited diseases. Patients with lymphoma, myelodysplasia and severe aplastic anemia have also been successfully transplanted with cord blood. Cord blood is obtained by syringing out the placenta through the umbilical cord at the time of childbirth, after the cord has been detached from the newborn. Collecting stem cells from umbilical blood and tissue is ethical, pain-free, safe and simple. When they are needed to treat your child later in life, there will be no rejection or incompatibility issues, as the procedure will be using their own cells. In contrast, stem cells from donors do have these potential problems. By consider about cord blood potency, cord blood banks (familial or public were established. In IRAN, four cord blood banks has activity, Shariati BMT center cord blood bank, Royan familial cord blood banks, Royan public cord blood banks and Iranian Blood Transfusion Organ cord blood banks. Despite 50,000 sample which storage in these banks, but the

  19. Neoadjuvant chemoradiotherapy of rectal carcinoma. Baseline hematologic parameters influencing outcomes

    Energy Technology Data Exchange (ETDEWEB)

    Hodek, Miroslav; Sirak, Igor; Paluska, Petr; Kopecky, Jindrich; Petera, Jiri; Vosmik, Milan [University Hospital in Hradec Kralove, Department of Oncology and Radiotherapy, Hradec Kralove (Czech Republic); Ferko, Alexander; Oerhalmi, Julius [University Hospital in Hradec Kralove, Department of Surgery, Hradec Kralove (Czech Republic); Hovorkova, Eva; Hadzi Nikolov, Dimitar [University Hospital in Hradec Kralove, Fingerland Department of Pathology, Hradec Kralove (Czech Republic)


    The link between the blood count and a systemic inflammatory response (SIR) is indisputable and well described. Pretreatment hematological parameters may predict the overall clinical outcomes in many types of cancer. Thus, this study aims to systematically evaluate the relationship between baseline blood count levels and treatment response in rectal cancer patients treated with neoadjuvant chemoradiotherapy. From 2009-2015, 173 patients with locally advanced rectal cancer were retrospectively enrolled in the study and analyzed. The baseline blood count was recorded in all patients 1 week before chemoradiation. Tumor response was evaluated through pathologic findings. Blood count levels which included RBC (red blood cells), Hb (hemoglobin), PLT (platelet count), neutrophil count, WBC (white blood cells), NLR (neutrophil-to-lymphocyte ratio), and PLR (platelet-to-lymphocyte ratio) were analyzed in relation to tumor downstaging, pCR (pathologic complete response), OS (overall survival), and DFS (disease-free survival). Hb levels were associated with a response in logistic regression analysis: pCR (p = 0.05; OR 1.04, 95 % CI 1.00-1.07); T downstaging (p = 0.006; OR 1.03, 95 % CI 1.01-1.05); N downstaging (p = 0.09; OR 1.02, 95 % CI 1.00-1.04); T or N downstaging (p = 0.007; OR 1.04, 95 % CI 1.01-1.07); T and N downstaging (p = 0.02; OR 1.02, 95 % CI 1.00-1.04); Hb and RBC were the most significant parameters influencing OS; PLT was a negative prognostic factor for OS and DFS (p = 0.008 for OS); an NLR value of 2.8 was associated with the greatest significance for OS (p = 0.03) and primary tumor downstaging (p = 0.02). Knowledge of pretreatment hematological parameters appears to be an important prognostic factor in patients with rectal carcinoma. (orig.) [German] Die Verbindung zwischen dem Blutbild und der systemischen Entzuendungsreaktion (''systemic inflammatory response'', SIR) ist unbestreitbar und gut beschrieben. Aufgrund der

  20. Biochemical nature of Russell Bodies. (United States)

    Mossuto, Maria Francesca; Ami, Diletta; Anelli, Tiziana; Fagioli, Claudio; Doglia, Silvia Maria; Sitia, Roberto


    Professional secretory cells produce and release abundant proteins. Particularly in case of mutations and/or insufficient chaperoning, these can aggregate and become toxic within or amongst cells. Immunoglobulins (Ig) are no exception. In the extracellular space, certain Ig-L chains form fibrils causing systemic amyloidosis. On the other hand, Ig variants lacking the first constant domain condense in dilated cisternae of the early secretory compartment, called Russell Bodies (RB), frequently observed in plasma cell dyscrasias, autoimmune diseases and chronic infections. RB biogenesis can be recapitulated in lymphoid and non-lymphoid cells by expressing mutant Ig-μ, providing powerful models to investigate the pathophysiology of endoplasmic reticulum storage disorders. Here we analyze the aggregation propensity and the biochemical features of the intra- and extra-cellular Ig deposits in human cells, revealing β-aggregated features for RB.

  1. Resetting predator baselines in coral reef ecosystems (United States)

    Bradley, Darcy; Conklin, Eric; Papastamatiou, Yannis P.; McCauley, Douglas J.; Pollock, Kydd; Pollock, Amanda; Kendall, Bruce E.; Gaines, Steven D.; Caselle, Jennifer E.


    What did coral reef ecosystems look like before human impacts became pervasive? Early efforts to reconstruct baselines resulted in the controversial suggestion that pristine coral reefs have inverted trophic pyramids, with disproportionally large top predator biomass. The validity of the coral reef inverted trophic pyramid has been questioned, but until now, was not resolved empirically. We use data from an eight-year tag-recapture program with spatially explicit, capture-recapture models to re-examine the population size and density of a key top predator at Palmyra atoll, the same location that inspired the idea of inverted trophic biomass pyramids in coral reef ecosystems. Given that animal movement is suspected to have significantly biased early biomass estimates of highly mobile top predators, we focused our reassessment on the most mobile and most abundant predator at Palmyra, the grey reef shark (Carcharhinus amblyrhynchos). We estimated a density of 21.3 (95% CI 17.8, 24.7) grey reef sharks/km2, which is an order of magnitude lower than the estimates that suggested an inverted trophic pyramid. Our results indicate that the trophic structure of an unexploited reef fish community is not inverted, and that even healthy top predator populations may be considerably smaller, and more precarious, than previously thought. PMID:28220895

  2. Gated integrator with signal baseline subtraction (United States)

    Wang, Xucheng


    An ultrafast, high precision gated integrator includes an opamp having differential inputs. A signal to be integrated is applied to one of the differential inputs through a first input network, and a signal indicative of the DC offset component of the signal to be integrated is applied to the other of the differential inputs through a second input network. A pair of electronic switches in the first and second input networks define an integrating period when they are closed. The first and second input networks are substantially symmetrically constructed of matched components so that error components introduced by the electronic switches appear symmetrically in both input circuits and, hence, are nullified by the common mode rejection of the integrating opamp. The signal indicative of the DC offset component is provided by a sample and hold circuit actuated as the integrating period begins. The symmetrical configuration of the integrating circuit improves accuracy and speed by balancing out common mode errors, by permitting the use of high speed switching elements and high speed opamps and by permitting the use of a small integrating time constant. The sample and hold circuit substantially eliminates the error caused by the input signal baseline offset during a single integrating window.

  3. Gated integrator with signal baseline subtraction

    Energy Technology Data Exchange (ETDEWEB)

    Wang, X.


    An ultrafast, high precision gated integrator includes an opamp having differential inputs. A signal to be integrated is applied to one of the differential inputs through a first input network, and a signal indicative of the DC offset component of the signal to be integrated is applied to the other of the differential inputs through a second input network. A pair of electronic switches in the first and second input networks define an integrating period when they are closed. The first and second input networks are substantially symmetrically constructed of matched components so that error components introduced by the electronic switches appear symmetrically in both input circuits and, hence, are nullified by the common mode rejection of the integrating opamp. The signal indicative of the DC offset component is provided by a sample and hold circuit actuated as the integrating period begins. The symmetrical configuration of the integrating circuit improves accuracy and speed by balancing out common mode errors, by permitting the use of high speed switching elements and high speed opamps and by permitting the use of a small integrating time constant. The sample and hold circuit substantially eliminates the error caused by the input signal baseline offset during a single integrating window. 5 figs.

  4. The LOFAR long baseline snapshot calibrator survey

    CERN Document Server

    Moldón, J; Wucknitz, O; Jackson, N; Drabent, A; Carozzi, T; Conway, J; Kapińska, A D; McKean, P; Morabito, L; Varenius, E; Zarka, P; Anderson, J; Asgekar, A; Avruch, I M; Bell, M E; Bentum, M J; Bernardi, G; Best, P; Bîrzan, L; Bregman, J; Breitling, F; Broderick, J W; Brüggen, M; Butcher, H R; Carbone, D; Ciardi, B; de Gasperin, F; de Geus, E; Duscha, S; Eislöffel, J; Engels, D; Falcke, H; Fallows, R A; Fender, R; Ferrari, C; Frieswijk, W; Garrett, M A; Grießmeier, J; Gunst, A W; Hamaker, J P; Hassall, T E; Heald, G; Hoeft, M; Juette, E; Karastergiou, A; Kondratiev, V I; Kramer, M; Kuniyoshi, M; Kuper, G; Maat, P; Mann, G; Markoff, S; McFadden, R; McKay-Bukowski, D; Morganti, R; Munk, H; Norden, M J; Offringa, A R; Orru, E; Paas, H; Pandey-Pommier, M; Pizzo, R; Polatidis, A G; Reich, W; Röttgering, H; Rowlinson, A; Scaife, A M M; Schwarz, D; Sluman, J; Smirnov, O; Stappers, B W; Steinmetz, M; Tagger, M; Tang, Y; Tasse, C; Thoudam, S; Toribio, M C; Vermeulen, R; Vocks, C; van Weeren, R J; White, S; Wise, M W; Yatawatta, S; Zensus, A


    Aims. An efficient means of locating calibrator sources for International LOFAR is developed and used to determine the average density of usable calibrator sources on the sky for subarcsecond observations at 140 MHz. Methods. We used the multi-beaming capability of LOFAR to conduct a fast and computationally inexpensive survey with the full International LOFAR array. Sources were pre-selected on the basis of 325 MHz arcminute-scale flux density using existing catalogues. By observing 30 different sources in each of the 12 sets of pointings per hour, we were able to inspect 630 sources in two hours to determine if they possess a sufficiently bright compact component to be usable as LOFAR delay calibrators. Results. Over 40% of the observed sources are detected on multiple baselines between international stations and 86 are classified as satisfactory calibrators. We show that a flat low-frequency spectrum (from 74 to 325 MHz) is the best predictor of compactness at 140 MHz. We extrapolate from our sample to sho...

  5. Arc melter demonstration baseline test results

    Energy Technology Data Exchange (ETDEWEB)

    Soelberg, N.R.; Chambers, A.G.; Anderson, G.L.; Oden, L.L.; O`Connor, W.K.; Turner, P.C.


    This report describes the test results and evaluation for the Phase 1 (baseline) arc melter vitrification test series conducted for the Buried Waste Integrated Demonstration program (BWID). Phase 1 tests were conducted on surrogate mixtures of as-incinerated wastes and soil. Some buried wastes, soils, and stored wastes at the INEL and other DOE sites, are contaminated with transuranic (TRU) radionuclides and hazardous organics and metals. The high temperature environment in an electric arc furnace may be used to process these wastes to produce materials suitable for final disposal. An electric arc furnace system can treat heterogeneous wastes and contaminated soils by (a) dissolving and retaining TRU elements and selected toxic metals as oxides in the slag phase, (b) destroying organic materials by dissociation, pyrolyzation, and combustion, and (c) capturing separated volatilized metals in the offgas system for further treatment. Structural metals in the waste may be melted and tapped separately for recycle or disposal, or these metals may be oxidized and dissolved into the slag. The molten slag, after cooling, will provide a glass/ceramic final waste form that is homogeneous, highly nonleachable, and extremely durable. These features make this waste form suitable for immobilization of TRU radionuclides and toxic metals for geologic timeframes. Further, the volume of contaminated wastes and soils will be substantially reduced in the process.

  6. Lorentz symmetry and Very Long Baseline Interferometry

    CERN Document Server

    Poncin-Lafitte, C Le; lambert, S


    Lorentz symmetry violations can be described by an effective field theory framework that contains both General Relativity and the Standard Model of particle physics called the Standard-Model extension (SME). Recently, post-fit analysis of Gravity Probe B and binary pulsars lead to an upper limit at the $10^{-4}$ level on the time-time coefficient $\\bar s^{TT}$ of the pure-gravity sector of the minimal SME. In this work, we derive the observable of Very Long Baseline Interferometry (VLBI) in SME and then we implement it into a real data analysis code of geodetic VLBI observations. Analyzing all available observations recorded since 1979, we compare estimates of $\\bar s^{TT}$ and errors obtained with various analysis schemes, including global estimations over several time spans and with various Sun elongation cut-off angles, and with analysis of radio source coordinate time series. We obtain a constraint on $\\bar s^{TT}=(-5\\pm 8)\\times 10^{-5}$, directly fitted to the observations and improving by a factor 5 pr...


    Institute of Scientific and Technical Information of China (English)

    王爱民; 韩光明; 封功能; 杨文平; 郭家豪; 王恬; 徐跑


    In order to determine the optimal levels of the lipid of GIFT strain Nile tilapia (Oreochromis niloticus), 630 GIFT with average weight of (2.63±0. 16) g were divided into six groups (1.73% lipid, 3.71% lipid, 5.69% lipid, 7.67% lipid, 9.64% lipid and 16.55% lipid) randomly. One with triplication was the control group fed with basal diet (1.73% lipid), the others were the treated groups fed with basal diet (1.73% lipid) supplemented with 2%, 4%, 6%, 8% and 15% fish oil, respectively. After rearing 90d, the growth, feed conversion ratio, nutrient apparent digestibility and blood biochemical parameter were determined. Results showed that, along with dietary lipid level raising, weight gain rate and specific growth rate had a trend of going up first then falling down (P<0.05), protein efficiency ratio improved (P<0.01) and feed conversion ratio reduced (P<0.01). Second-order regression of weight gain rate on concentrations of dietary lipid indicated that the optimal dietary lipid for maximal growth of GIFT tilapia was about 9.34%. Crude protein and dry-matter digestibility had no significant effected by dietary lipid levels (P>0.05). Crude lipid and phosphorus digestibility raised significantly with dietary lipid level increasing (P<0.05). Albumin and albumin/globulin ratio in 1.73% group (this group did not added fish oil) was significantly higher than other group (P<0.05). Along with dietary lipid level raising, cholesterol and alkaline phosphatase of fish serum raised significantly (P<0.01). Dietary lipid level had significantly influence on blood glucose (P<0.05), and there was no significantly influence on the content of triglyceride and the activities of glutamic-pyruvic transaminase and glutamic-oxaloacetic transaminase (P>0.05). It indicated that the level of lipid in the dietary could improve the growth, the apparent digestibilities of crude lipid and phosphorus to some degree, but excessive amount of lipid

  8. 不同免疫状态对肉仔鸡血液生化指标和细胞免疫应答的影响%Effects of Different Immune Status on Blood Biochemical Parameters and Cellular Immune Response in Broiler Chickens

    Institute of Scientific and Technical Information of China (English)

    冯焱; 杨小军; 王筱霏; 覃定奎; 尹瑞卿; 姚军虎


    [目的]研究不同免疫状态对肉仔鸡血液生化指标和细胞免疫应答的影响.[方法]选用健康情况良好1日龄AA肉公鸡180只,随机分为4组,每组5个重复,每重复9只.设定不同免疫应激模型,为无免疫组即对照组(no vaccine,NV),常规免疫组(conventional vaccine,CV),以及在常规免疫基础上注射细菌脂多糖的免疫亢进组(LPS)和环磷酰胺的免疫抑制组(CYP).采用CD3/CD4/CD8三色流式细胞术、MTT法检测肉鸡免疫状态和非免疫状态下外周血中T淋巴细胞及其亚群的变化.[结果]与NV组相比,肉仔鸡的体增重和采食量均显著降低LPS组和CYP组(P<0.05),各处理料重比无显著差异(P>0.05),NV组与CV组生产性能指标无显著差异(P> 0.05),各日龄处理间血清TP、GLU和CHL)含量均无显著差异(P>0.05).第21和35天,LPS组和CYP组血清ALB含量显著低于CV组和NV组(P<0.05);第21天,LPS组血清α-AGP和BUN含量显著低于NV组(P<0.05),CPY和LPS均抑制T、B淋巴细胞增殖,LPS组CD4+/CD8+T细胞百分比显著低于NV组(P<0.05).[结论]未进行免疫肉鸡可维持机体正常的生长性能和细胞免疫稳态,LPS和CYP的刺激可降低肉鸡采食量和体增重;随日龄增加肉鸡对CYP刺激的耐受性有所上升,LPS刺激可增强肉鸡细胞免疫应答反应.%[Objective] This experiment was conducted to determine the effects of different immune statuses on blood biochemical parameters and cellular immune response of broiler chickens. [Method] One hundred and eighty 1 -day-old AA broilers were allocated into four groups. Each treatment had 5 replicate cages of 9 broilers per replicate pen. This study will set different immune stress models, including the control group (no vaccines, NV), conventional vaccines group (CV), immune hyperthyroidism group (LPS) and immune suppression group (CYP). The T lymphocytes and subgroups were isolated from periphery blood from immunized and unvaccinated birds and were

  9. 玉米酒精糟和复合酶对生长育肥猪血液生化指标和肠道健康的影响%Effects of corn DDGS and compound enzyme preparations on the blood biochemical indicator and gut health in growing-finishing pigs

    Institute of Scientific and Technical Information of China (English)

    王潇; 李根来; 姚文


    本试验旨在探讨生长育肥猪饲粮中添加一定量的玉米酒精糟(DDGS)和复合酶对生长育肥猪血液生化指标和肠道健康的影响.150头70日龄杜×长×大三元杂交商品猪,根据体重和性别随机分成3个处理,每个处理5个重复,每个重复10头猪,进行两阶段饲养.对照组饲喂玉米-豆粕型日粮,试验1组和2组在生长期和育肥期饲料中分别以10%和15%的玉米DDGS替代部分玉米和豆粕,试验2组添加200 g/T的复合酶.于试验开始后的第24,48,70和90天,每栏随机选取一头猪,直肠无菌采集粪样以检测其中的乳酸菌和大肠杆菌的数量.试验结束时,每栏随机选取一头猪进行屠宰,采集血液和组织样品,进行血液生化指标的测定和小肠形态的观察.结果显示,添加玉米DDGS和复合酶对肝、脾指数和血液AKP、γ-GT、GOT和GPT水平没有显著影响(P>0.05);试验组猪的十二指肠和回肠绒毛高度与隐窝深度之比值大于对照组,且试验2组在育肥期达到显著水平(P<0.05).试验2组育肥期粪样中乳酸菌数量较对照组显著升高(P<0.05),试验1组生长期粪样中大肠杆菌数量则显著低于对照组(P<0.05).因此,本研究中玉米DDGS的添加量不仅没有对生长育肥猪的肠道形态和肝脏等器官产生明显的毒害作用,而且结合复合酶的使用有利于提高十二指肠和回肠绒毛高度与隐窝深度之比值,一定程度上促进了生长育肥猪后肠中乳酸菌的生长,抑制了大肠杆菌的生长,有助于维持肠道健康.%To investigate the effect of corn DDGS and compound enzyme preparations on blood biochemical indicator and gut health in growing-finishing pigs, 150 pigs (DurocxLandracexYorkshire) at the age of 70 days were randomly allotted to three dietary treatments on the basis of body weight and gender. Control diets were a basic corn-soybean meal diet. Treatments 1 and 2 were corn-soybean meal-DDGS diets with 10% corn DDGS

  10. Qualidade de ovos, parâmetros bioquímicos sanguíneos e desenvolvimento do aparelho reprodutor de poedeiras comerciais Lohmann LSL alimentadas com níveis crescentes de lisina digestível - DOI: 10.4025/actascianimsci.v30i1.3596 Egg quality, blood biochemical parameters and reproductive tract development for Lohmann LSL hens fed increasing levels of digestible lysine - DOI: 10.4025/actascianimsci.v30i1.3596

    Directory of Open Access Journals (Sweden)

    Anúzia Barini Nunes


    Full Text Available O objetivo deste trabalho foi avaliar os efeitos de lisina para poedeiras Lohmann LSL sobre a qualidade dos ovos, parâmetros bioquímicos sanguíneos e mensurações do aparelho reprodutor. Cento e sessenta poedeiras com 24 semanas foram submetidas a níveis crescentes de lisina digestível (600, 700, 800 e 900 mg Lys kg-1 de ração até 48 semanas de idade. As dietas foram isoenergéticas e isonutritivas com 15,8%PB e 2.800 kcal EM kg-1. Delineamento inteiramente casualizado foi adotado com quatro tratamentos e quatro repetições. Foram analisados: percentagem de casca, de gema e albúmen, gravidade específica dos ovos, unidade Haugh, índice gema, sólidos totais da gema, do albúmen e do ovo, parâmetros sanguíneos e mensuração do aparelho reprodutor. Os resultados foram submetidos à análise de variância e de regressão polinomial (5%, utilizando o programa SAS, e para os resultados sanguíneos e do aparelho reprodutor, foram analisados em esquema de parcelas subdivididas. Os níveis de lisina não influenciaram os resultados para qualidade dos ovos. Houve aumento no peso do aparelho reprodutor com o avançar da idade. Os resultados do parâmetro sanguíneo não foram influenciados; já nas semanas, observa-se aumento na albumina, uratos e uratina. O nível de 600 mg kg-1 pode ser recomendado para poedeiras no período avaliado.In this experiment, the effect of lysine levels on egg quality, biochemical parameters and measurements of the reproductive tract on the diets of Lohmann LSL laying hens diets were evaluated. One hundred-sixty hens with 24 weeks of age were submitted to increasing levels of digestible lysine (600, 700, 800 e 900 mg Lys kg-1 of feed until 48 weeks of age. The diets were isoenergetic and isonutritive. A completely randomized design was used, with four treatments and four replicates. Percentages of eggshell, yolk and albumen, specific egg gravity, yolk index, total yolk, albumen and egg solids, blood parameters

  11. Ethylmalonic encephalopathy: application of improved biochemical and molecular diagnostic approaches. (United States)

    Drousiotou, A; DiMeo, I; Mineri, R; Georgiou, Th; Stylianidou, G; Tiranti, V


    Ethylmalonic encephalopathy (EE, OMIM # 602473) is an autosomal recessive metabolic disorder of infancy affecting the brain, the gastrointestinal tract and peripheral vessels. It is caused by a defect in the ETHE1 gene product, which was recently shown to be part of a metabolic pathway devoted to sulphide detoxification. We report the application of improved biochemical and molecular approaches to the diagnosis of three cases of EE from two unrelated Cypriot families. The children presented all the typical biochemical hallmarks of the disease including elevated lactate and butyrylcarnitine in blood and elevated urinary excretion of ethylmalonic acid, 2-methylsuccinate, isobutyrylglycine and isovalerylglycine. We also detected an elevated level of thiosulphate in urine, which we propose as an additional biochemical marker of the disease. The proband of the first family was shown to be a compound heterozygote for a missense mutation in exon 5, L185R, and a deletion of exon 4. The deletion was identified using quantitative real-time polymerase chain reaction (qRT-PCR). Using the same technique, the proband of the second family was found to be homozygous for the exon 4 deletion. A prenatal diagnosis was performed for the second family using qRT-PCR, thus establishing the usefulness of RT-PCR in prenatal diagnosis.

  12. Importance of Biochemical Markers in Postmenopausal and Senile Osteoporosis

    Directory of Open Access Journals (Sweden)

    Deniz Evcik


    Full Text Available Recently, the biochemical markers are widely used in order to evaluate the bone turnover. This study was planned to investigate the role of biochemical markers and Bone Mineral Density(BMD in postmenopausal (PMO and senile osteoporosis (SO patients. A total of 86 patients( 44 PMO, 42 SO, ages ranged between 39-79 were included in this study. Alkaline phosphatase (ALP and osteocalcin levels were determined from blood samples. Urinary deoxypyridinoline(Dpd and creatinine(cr concentration were examined and the ratio of Dpd/cr was calculated. Also BMD of the patients were measured from L1-L4 and proximal femur and t score were determined. There was no statistical difference in ALP levels between two groups. Osteocalcine and Dpd/cr levels were statistically increased in PMO group(p<0.001. According to BMD t score which was measured from proximal femur was significantly higher in SO patients(p<0.05. Our results show that biochemical markers are useful for the assessment of high-turnover osteoporosis.

  13. Human choline dehydrogenase: medical promises and biochemical challenges. (United States)

    Salvi, Francesca; Gadda, Giovanni


    Human choline dehydrogenase (CHD) is located in the inner membrane of mitochondria primarily in liver and kidney and catalyzes the oxidation of choline to glycine betaine. Its physiological role is to regulate the concentrations of choline and glycine betaine in the blood and cells. Choline is important for regulation of gene expression, the biosynthesis of lipoproteins and membrane phospholipids and for the biosynthesis of the neurotransmitter acetylcholine; glycine betaine plays important roles as a primary intracellular osmoprotectant and as methyl donor for the biosynthesis of methionine from homocysteine, a required step for the synthesis of the ubiquitous methyl donor S-adenosyl methionine. Recently, CHD has generated considerable medical attention due to its association with various human pathologies, including male infertility, homocysteinuria, breast cancer and metabolic syndrome. Despite the renewed interest, the biochemical characterization of the enzyme has lagged behind due to difficulties in the obtainment of purified, active and stable enzyme. This review article summarizes the medical relevance and the physiological roles of human CHD, highlights the biochemical knowledge on the enzyme, and provides an analysis based on the comparison of the protein sequence with that of bacterial choline oxidase, for which structural and biochemical information is available.

  14. The LBNO long-baseline oscillation sensitivities with two conventional neutrino beams at different baselines

    CERN Document Server

    Agarwalla, S.K.; Aittola, M.; Alekou, A.; Andrieu, B.; Antoniou, F.; Asfandiyarov, R.; Autiero, D.; Besida, O.; Balik, A.; Ballett, P.; Bandac, I.; Banerjee, D.; Bartmann, W.; Bay, F.; Biskup, B.; Blebea-Apostu, A.M.; Blondel, A.; Bogomilov, M.; Bolognesi, S.; Borriello, E.; Brancus, I.; Bravar, A.; Buizza-Avanzini, M.; Caiulo, D.; Calin, M.; Calviani, M.; Campanelli, M.; Cantini, C.; Cata-Danil, G.; Chakraborty, S.; Charitonidis, N.; Chaussard, L.; Chesneanu, D.; Chipesiu, F.; Crivelli, P.; Dawson, J.; De Bonis, I.; Declais, Y.; del Amo Sanchez, P.; Delbart, A.; Di Luise, S.; Duchesneau, D.; Dumarchez, J.; Efthymiopoulos, I.; Eliseev, A.; Emery, S.; Enqvist, T.; Enqvist, K.; Epprecht, L.; Erykalov, A.N.; Esanu, T.; Franco, D.; Friend, M.; Galymov, V.; Gavrilov, G.; Gendotti, A.; Giganti, C.; Gilardoni, S.; Goddard, B.; Gomoiu, C.M.; Gornushkin, Y.A.; Gorodetzky, P.; Haesler, A.; Hasegawa, T.; Horikawa, S.; Huitu, K.; Izmaylov, A.; Jipa, A.; Kainulainen, K.; Karadzhov, Y.; Khabibullin, M.; Khotjantsev, A.; Kopylov, A.N.; Korzenev, A.; Kosyanenko, S.; Kryn, D.; Kudenko, Y.; Kuusiniemi, P.; Lazanu, I.; Lazaridis, C.; Levy, J.M.; Loo, K.; Maalampi, J.; Margineanu, R.M.; Marteau, J.; Martin-Mari, C.; Matveev, V.; Mazzucato, E.; Mefodiev, A.; Mineev, O.; Mirizzi, A.; Mitrica, B.; Murphy, S.; Nakadaira, T.; Narita, S.; Nesterenko, D.A.; Nguyen, K.; Nikolics, K.; Noah, E.; Novikov, Yu.; Oprima, A.; Osborne, J.; Ovsyannikova, T.; Papaphilippou, Y.; Pascoli, S.; Patzak, T.; Pectu, M.; Pennacchio, E.; Periale, L.; Pessard, H.; Popov, B.; Ravonel, M.; Rayner, M.; Resnati, F.; Ristea, O.; Robert, A.; Rubbia, A.; Rummukainen, K.; Saftoiu, A.; Sakashita, K.; Sanchez-Galan, F.; Sarkamo, J.; Saviano, N.; Scantamburlo, E.; Sergiampietri, F.; Sgalaberna, D.; Shaposhnikova, E.; Slupecki, M.; Smargianaki, D.; Stanca, D.; Steerenberg, R.; Sterian, A.R.; Sterian, P.; Stoica, S.; Strabel, C.; Suhonen, J.; Suvorov, V.; Toma, G.; Tonazzo, A.; Trzaska, W.H.; Tsenov, R.; Tuominen, K.; Valram, M.; Vankova-Kirilova, G.; Vannucci, F.; Vasseur, G.; Velotti, F.; Velten, P.; Venturi, V.; Viant, T.; Vihonen, S.; Vincke, H.; Vorobyev, A.; Weber, A.; Wu, S.; Yershov, N.; Zambelli, L.; Zito, M.


    The proposed Long Baseline Neutrino Observatory (LBNO) initially consists of $\\sim 20$ kton liquid double phase TPC complemented by a magnetised iron calorimeter, to be installed at the Pyh\\"asalmi mine, at a distance of 2300 km from CERN. The conventional neutrino beam is produced by 400 GeV protons accelerated at the SPS accelerator delivering 700 kW of power. The long baseline provides a unique opportunity to study neutrino flavour oscillations over their 1st and 2nd oscillation maxima exploring the $L/E$ behaviour, and distinguishing effects arising from $\\delta_{CP}$ and matter. In this paper we show how this comprehensive physics case can be further enhanced and complemented if a neutrino beam produced at the Protvino IHEP accelerator complex, at a distance of 1160 km, and with modest power of 450 kW is aimed towards the same far detectors. We show that the coupling of two independent sub-MW conventional neutrino and antineutrino beams at different baselines from CERN and Protvino will allow to measure ...

  15. Immunoelectrophoresis - blood (United States)

    IEP - serum; Immunoglobulin electrophoresis - blood; Gamma globulin electrophoresis; Serum immunoglobulin electrophoresis; Amyloidosis - electrophoresis serum; Multiple myeloma - serum electrophoresis; Waldenström - serum electrophoresis

  16. Diazinon toxicity affects histophysiological and biochemical parameters in rabbits. (United States)

    Yehia, Mona A H; El-Banna, Sabah G; Okab, Aly B


    Diazinon is a widely used pesticide in agriculture. So, the current work aimed to investigate the effects of diazinon exposure on some physiological and biochemical parameters, as well as, histopathological changes and histochemical acetyl-cholinesterase activity (AChE). The red Baladi rabbits were dipped into water (Control Group), diazinon at low concentrations of 0.6 mg diazinon low concentration (DLC) or high concentration of 3mg diazinon high concentration (DHC) dissolved in 1l of water for 10s. Treatment was repeated after 10 days and animals were sacrificed between 0 and 21 days after the second treatment. Blood analysis revealed that Red blood cells (RBC's), hemoglobin (Hb) and plasma total protein (TP) were significantly decreased in both diazinon concentrations (Pliver/ body weight and cytochrome P-450 were decreased in both concentrations (Pliver, kidney and brain were observed after DHC dipping. Glycogen content was decreased in liver and increased in kidney Bowman's capsule. Furthermore, AChE activity was inhibited in brain tissue, decreased in liver cells, but gradually increased in kidney glomerular cells. Therefore, kidney and brain were highly affected by diazinon exposure compared with the liver. Exposure of animals to diazinon caused extensive changes in physiological, biochemical, and histopathological parameters as well as histochemical AChE. So, contact exposure of diazinon leads to negative response on animal health.

  17. Associative learning in biochemical networks. (United States)

    Gandhi, Nikhil; Ashkenasy, Gonen; Tannenbaum, Emmanuel


    It has been recently suggested that there are likely generic features characterizing the emergence of systems constructed from the self-organization of self-replicating agents acting under one or more selection pressures. Therefore, structures and behaviors at one length scale may be used to infer analogous structures and behaviors at other length scales. Motivated by this suggestion, we seek to characterize various "animate" behaviors in biochemical networks, and the influence that these behaviors have on genomic evolution. Specifically, in this paper, we develop a simple, chemostat-based model illustrating how a process analogous to associative learning can occur in a biochemical network. Associative learning is a form of learning whereby a system "learns" to associate two stimuli with one another. Associative learning, also known as conditioning, is believed to be a powerful learning process at work in the brain (associative learning is essentially "learning by analogy"). In our model, two types of replicating molecules, denoted as A and B, are present in some initial concentration in the chemostat. Molecules A and B are stimulated to replicate by some growth factors, denoted as G(A) and G(B), respectively. It is also assumed that A and B can covalently link, and that the conjugated molecule can be stimulated by either the G(A) or G(B) growth factors (and can be degraded). We show that, if the chemostat is stimulated by both growth factors for a certain time, followed by a time gap during which the chemostat is not stimulated at all, and if the chemostat is then stimulated again by only one of the growth factors, then there will be a transient increase in the number of molecules activated by the other growth factor. Therefore, the chemostat bears the imprint of earlier, simultaneous stimulation with both growth factors, which is indicative of associative learning. It is interesting to note that the dynamics of our model is consistent with certain aspects of

  18. Understanding Blood Counts (United States)

    ... Lab and Imaging Tests Understanding Blood Counts Understanding Blood Counts Understanding Blood Counts SHARE: Print Glossary Blood cell counts give ... your blood that's occupied by red cells. Normal Blood Counts Normal blood counts fall within a range ...

  19. Biochemical genetic markers in sugarcane. (United States)

    Glaszmann, J C; Fautret, A; Noyer, J L; Feldmann, P; Lanaud, C


    Isozyme variation was used to identify biochemical markers of potential utility in sugarcane genetics and breeding. Electrophoretic polymorphism was surveyed for nine enzymes among 39 wild and noble sugarcane clones, belonging to the species most closely related to modern varieties. Up to 114 distinct bands showing presence versus absence type of variation were revealed and used for qualitative characterization of the materials. Multivariate analysis of the data isolated the Erianthus clone sampled and separated the Saccharum spontaneum clones from the S. robustum and S. officinarum clones; the latter two were not differentiated from one another. The analysis of self-progenies of a 2n=112 S. spontaneum and of a commercial variety showed examples of mono- and polyfactorial segregations. Within the progeny of the variety, co-segregation of two isozymes frequent in S. spontaneum led to them being assigned to a single chromosome initially contributed by a S. spontaneum donor. This illustrates how combined survey of ancestral species and segregation analysis in modern breeding materials should permit using the lack of interspecific cross-over to establish linkage groups in a sugarcane genome.


    CERN Multimedia

    SC Unit


    A blood donation, organized by EFS (Etablissement Français du Sang) of Annemasse will take place On Wednesday 12 November 2008, from 8:30 to 16:00, at CERN Restaurant 2 If possible, please, bring your blood group Card.

  1. Blood donation

    CERN Multimedia

    GS Department


    A blood donation is organised by the Cantonal Hospital of Geneva On Thursday 19 March 2009 from 9 a.m. to 5 p.m. CERN RESTAURANT 2 Number of donations during the last blood donations :135 donors in July 2008 122 donors in November 2008 Let’s do better in 2009 !!! Give 30 minutes of your time to save lives...

  2. Tainted blood

    DEFF Research Database (Denmark)

    Deleuran, Ida; Sheikh, Zainab Afshan; Hoeyer, Klaus


    study of the historical rise and current workings of safety practices in the Danish blood system. Here, we identify a strong focus on contamination in order to avoid 'tainted blood', at the expense of working with risks that could be avoided through enhanced blood monitoring practices. Of further...... significance to this focus are the social dynamics found at the heart of safety practices aimed at avoiding contamination. We argue that such dynamics need more attention, in order to achieve good health outcomes in transfusion medicine. Thus, we conclude that, to ensure continuously safe blood systems, we...... need to move beyond the bifurcation of the social and medical aspects of blood supply as two separate issues and approach social dynamics as key medical safety questions....

  3. 植物挥发油及活性成分与富马酸钠共同添加对湖羊血液生化和抗氧化指标的影响%Effects of essential oil and their active component combinations along with fumarate on blood biochemical index and anti-oxidative status in Hu sheep

    Institute of Scientific and Technical Information of China (English)

    林波; 梁权; 纪苗苗; 刘建新


    用装有瘤胃瘘管的4头湖羊探讨植物挥发油及其活性成分和富马酸钠添加对湖羊血液生化和抗氧化指标的影响。采用4×4拉丁方设计,设4个处理,处理1饲喂基础日粮,处理2添加1g混合挥发油(牛至油、肉桂油、丁香油和柠檬油按等比例组成)与25g富马酸一钠,处理3添加0.5g混合挥发油活性成分(香芹酚、肉桂醛、丁香酚和柠檬醛等比例混合)与25g富马酸一钠,处理4添加1g混合挥发油活性成分与25g富马酸一钠。每期包括10d预试和8d正试验。结果发现,添加混合挥发油组和高量活性成分(1g/d)组显著提高了血清白蛋白含量,降低了白球蛋白比(P〈0.05),但对其他指标影响不显著;而低量活性成分(0.5g/d)组则显著降低了球蛋白、碱性磷酸酶、谷草转氨酶、肌酐和胆固醇的含量(P〈0.05)。高量和低量活性成分组相比对照组均显著降低了谷胱苷肽过氧化物酶、超氧化物歧化酶活性和总抗氧化能力(P〈0.05),但低量活性成分组降低了丙二醛含量,而混合挥发油组则与对照组无显著差异。结果表明,添加1g/d挥发油或活性成分降低了白球蛋白比,而有提高球蛋白含量的趋势,1g/d活性成分还有提高碱性磷酸酶和谷丙转氨酶活性的趋势,可能对湖羊肝脏有一定的影响;而0.5g/d活性成分降低了球蛋白、碱性磷酸酶、谷草转氨酶等指标,可能有利于肝脏功能;添加挥发油不影响肾脏功能、机体脂肪和蛋白代谢,即添加挥发油对湖羊抗氧化能力影响有限,0.5g/d挥发油活性成分添加并未影响湖羊机体健康。%Four rumen fistulated Hu sheep were used to investigate the anti-oxidative status and blood biochemical parameters influenced by addition of mixture of essential oils and their active components along with fumarate.A 4×4 Latin Square design involved four treatments: control(C) was basal diet with 25 g

  4. Effects of different dietary carbohydrate levels on growth performance and blood biochemical parameters of juvenile GIFT tilapia(Oreochromis niloticus)%饲料碳水化合物水平对吉富罗非鱼幼鱼生长性能和血液主要生化指标的影响

    Institute of Scientific and Technical Information of China (English)

    吴凡; 文华; 蒋明; 刘伟; 刘波; 田娟; 杨长庚


    【Objective】 This study investigated the influences of different dietary carbohydrate(CBH) levels on growth performance,body composition and blood biochemical parameters of juvenile GIFT tilapia(Oreochromis niloticus),to detect the suitable addition amount of CBH to the diet of GIFT tilapia.【Method】 5 iso-lipidic(7.0% crude lipid) diet groups were formulated with different CBH mass fractions:20%,27%,34%,41%,and 48%,respectively.Each diet was randomly fed to triplicate groups of 30 juvenile GIFT tilapia with average weights of(4.77±0.52) g for 10 weeks.【Result】 The weight gain rates(WGR) and specific growth rates(SGR) of the diet group with 41% CBH were significant higher than those of the other four groups(P0.05).The feed conversion rates(FCR) of the diet group with 27% CBH were the lowest in the all groups.The hepatopancreas somatic indexes(HSI) and viscerosomatic indexes(VSI) of test fishes increased significantly as the increase of dietary CBH levels.Protein efficiency ratios(PER) and protein productive values(PPV) of the diet group with 41% CBH were significant higher than those of groups with 20%,27% and 34% CBH(P0.05).Crude protein contents in whole-body and liver of fishes decreased as the increase of dietary CBH levels,while body and liver lipid contents showed converse trends.The contents of serum cholesterol(CHO) and triglyceride(TG) and the activities of aspartate aminotransferase(AST) and alanine aminotransferase(ALT) of the fishes fed with 48% CBH diet were significant higher than other treatments.【Conclusion】 The results indicated that dietary CBH could reduce the protein dosage for juvenile GIFT tilapia and the suggested optimal dietary CBH level was 41%.However,exorbitant dietary CBH intake(such as 48%) might result in liver fat accumulation,functional disorder and hyperlipemia.%【目的】研究不同碳水化合物(CBH)水平对吉富罗非鱼幼鱼生长性能、体成分和血液生化指标的影响,以确定其适宜添

  5. B→O blood conversion

    Institute of Scientific and Technical Information of China (English)


    To maintain a constant supply of "universal blood type", or group O red blood cells, has benefit in specialized transfusion condition. In this study, α-galactosidase cDNA was cloned from Catimor coffee bean grown in Hainan island, China, by the RT-PCR method. We have constructed a vector for α-galactosidase cDNA expression and transferred α-galactosidase cDNA into Pichia pastoris GS115 cells by electroporation. The recombinant α-galactosidase (r-α-GalE) was purified by cation ion exchange chromatography. After studying the biochemical characters of r-α-GalE, we have succeeded in converting human erythrocytes from group B to group O. The animal experiment showed that transfusion of enzymetically converted group O red blood cells (ECORBC) was perfectly safe.

  6. Effects of parsley (Petroselinum crispum) on the liver of diabetic rats: a morphological and biochemical study. (United States)

    Bolkent, S; Yanardag, R; Ozsoy-Sacan, O; Karabulut-Bulan, O


    Parsley is used by diabetics in Turkey to reduce blood glucose. The present study aims to investigate both the morphological and biochemical effects of parsley on liver tissue. Rat hepatocytes were examined by light and electron microscopy. Degenerative changes were observed in the hepatocytes of diabetic rats. These degenerative changes were significantly reduced or absent in the hepatocytes of diabetic rats treated with parsley. Blood glucose levels, alanine transaminase and alkaline phosphatase were observed to be raised in diabetic rats. Diabetic rats treated with parsley demonstrated significantly lower levels of blood glucose, alanine transaminase and alkaline phosphatase. The present study suggests that parsley demonstrates a significant hepatoprotective effect in diabetic rats.

  7. Numerical simulations of a reduced model for blood coagulation (United States)

    Pavlova, Jevgenija; Fasano, Antonio; Sequeira, Adélia


    In this work, the three-dimensional numerical resolution of a complex mathematical model for the blood coagulation process is presented. The model was illustrated in Fasano et al. (Clin Hemorheol Microcirc 51:1-14, 2012), Pavlova et al. (Theor Biol 380:367-379, 2015). It incorporates the action of the biochemical and cellular components of blood as well as the effects of the flow. The model is characterized by a reduction in the biochemical network and considers the impact of the blood slip at the vessel wall. Numerical results showing the capacity of the model to predict different perturbations in the hemostatic system are discussed.

  8. What Happens to Donated Blood? (United States)

    ... week. Learn About Blood Blood Facts and Statistics Blood Components Whole Blood and Red Blood Cells Platelets Plasma ... About Blood Blood Facts and Statistics Blood Types Blood Components What Happens to Donated Blood Blood and Diversity ...

  9. Effect of fire smoke on some biochemical parameters in firefighters of Saudi Arabia

    Directory of Open Access Journals (Sweden)

    Rezq Ameen M


    Full Text Available Abstract Background Firefighters who are facing fires, are frequently exposed to hazardous materials including carbon monoxide, hydrogen cyanide, hydrogen chloride, benzene, sulphur dioxide, etc. This study aimed to evaluate some relevant serum biochemical and blood hematological changes in activity involved firefighters in comparison to normal subjects. Subjects and Methods Two groups of male firefighters volunteered to participate in the study. The first included 28 firefighters from Jeddah, while the second included 21 firefighters from Yanbu, with overall age ranged 20–48 years. An additional group of 23 male non-firefighters volunteered from both cities as normal control subjects, of age range 20–43 years. Blood samples were collected from all volunteer subjects and investigated for some relevant serum biochemical and blood hematological changes. Results The results obtained showed that, there were statistically significant differences in liver function, kidney function, serum lipid profile, cortisol, creatine kinase, lactate dehydrogenase, iron and its biologically active derivatives, and blood picture in firefighters as compared with the normal control group. These results indicate that, fire smoke mainly affects serum biochemical and blood hematological parameters. Such results might point out to the need for more health protective and prophylactic measures to avoid such hazardous health effects that might endanger firefighters under their highly drastic working conditions. Conclusion Besides using of personal protective equipments for firefighters to protect them against exposure to toxic materials of fire smoke, it is recommended that, firefighters must be under continuous medical follow up through a standard timetabled medical laboratory investigations to allow for early detection of any serum biochemical or blood hematological changes that might happen during their active service life and to allow for early treatment whenever

  10. Effects Of Music Therapy On Clinical And Biochemical Parameters Of Metabolic Syndrome

    Directory of Open Access Journals (Sweden)



    Full Text Available Background: Music therapy is a new approach being used for the management of metabolic abnormalities and stress related illness.Objective: To study the effect of Music therapy on various clinical and biochemical parameters of Metabolic Syndrome.Methods: This cross sectional study was carried out on 100 patients of metabolic syndrome selected randomly. These patients were divided into two equal groups after age, sex adjustment. In control group (group I 50 patients underwent the conventional treatment. 50 patients in study group were treated with supervised music protocol along with conventional treatment. The Body Mass Index, ;Waist-Hip ratio, Blood pressure, Fasting blood sugar were monitored weekly while HbA1c and lipid profile were determined at the baseline and after three months of exposure to music therapy. Statistical analysis was performed by employing student t- test.Results: In the study group there was a significant decrease in BMI (27.18±5.02 to 25.44±3.49 kg/m2, p<0.05, waist hip ratio (0.95±0.05 to 0.93±0.05 cm, p<0.05, Fasting blood sugar (196.00±47.80mg/ dl to152.00±16.19mg/dl , p<0.001, HbA1c (8.41±1.31% to 7.08±0.78 % p<0.001, Systolic Blood Pressure (151.00±12.10 to 136±9.04 mmHg p<0.001, Diastolic Blood Pressure (94±4.80 to 86.44±3.16 mmHg, p<0.01, Mean serum cholesterol (257.80±18.92 to 229.12±17.82mg/dl, p<0.001 and triglycerides (180.86±14.04 to 136.50±8.92mg/dl, p<0.001, LDL (167.97±14.40 to 140.20±15.41mg/dl, p<0.001, and VLDL (33.60±2.88 to 28.04±3.08mg/dl, p<0.001 and increase in HDL (33.32±3.38 to 39.71±3.41mg/dl, p<0.001, when compared with those of control group not receiving the music therapy along with the conventional treatment.Conclusion: The promising outcomes of Music therapy showed that it may be considered as a useful adjunct to conventional treatment in management of the metabolic syndrome. This study advocates music therapy to establish it from a general well being concepts to a

  11. Epidemiology of cardiovascular risk factors in Greece: aims, design and baseline characteristics of the ATTICA study

    Directory of Open Access Journals (Sweden)

    Chrysohoou Christina


    Full Text Available Abstract Background In an attempt to evaluate the levels of several cardiovascular risk factors in Greece we conducted a population-based health and nutrition survey, the "ATTICA study". In this work we present the design and the methodology of the study, as well as the status of various baseline characteristics of the participants. Methods From May 2001 to December 2002 we randomly enrolled 1514 adult men and 1528 adult women, stratified by age – gender (census 2000, from the greater area of Athens. More than 300 demographic, lifestyle, behavioral, dietary, clinical and biochemical variables have been recorded. Results Regarding the frequency of the classical cardiovascular risk factors we observed that 51% of men and 39% of women reported smokers (p Conclusions The prevalence of the common cardiovascular risk factors in our population seems high. As a consequence a considerable proportion of Greek adults are at "high-risk" for future cardiovascular events.

  12. Evaluation of nutritional and biochemical parameters in spontaneously hypertensive rats following antihypertensive treatment

    Directory of Open Access Journals (Sweden)

    Joanna Suliburska


    Full Text Available Introduction. One side effect of antihypertensive drugs is their impact on nutritional status and metabolism. The purpose of this study was to assess the nutritional and biochemical parameters in spontaneously hypertensive rats following treatment with antihypertensive drugs. Material and methods. The experiment was performed on 50 male spontaneously hypertensive rats (SHR, which were assigned to fi ve groups: control (C, with perindopril (PR, with metoprolol (MT, with indapamide (ID, and with amlodipine (AM. All rats were provided ad libitum standard diet (with or without drugs and distilled water. After 45 days, the animals were weighed and killed. Liver, kidney, heart, spleen, pancreas, and blood samples were collected. Concentrations of glucose, cholesterol, triglycerides, and albumin were assayed in serum. Morphology parameters, such as white blood cell, red blood cell, hematocrit, and lymphocyte counts were measured in the blood. Blood pressure was measured using a tail-cuff plethysmograph. Results. The results obtained indicate that the hypotensive drugs under investigation had no effect on the selected nutritional parameters. Perindopril signifi cantly decreased the relative mass of the heart and amlodipine markedly decreased the relative mass of the pancreas. A markedly higher concentration of glucose in the group with indapamid, and a signifi cantly lower concentration of triglycerides in the group with metoprolol, were observed. Indapamide and amlodipine markedly increased the value of red blood cells and hematocrit in the blood of SHR. Conclusions. Long-term therapy with antihypertension drugs may infl uence tissue mass and biochemical and morphological status in the body.


    Directory of Open Access Journals (Sweden)

    Ekeanyanwu Raphael Chukwuma


    Full Text Available The nutritive constituents of the seeds of Monodora tenuifolia were analyzed to augment the available information on Monodora tenuifolia research. Blood glucose and lipid profile were investigated on the flavonoid rich fraction of M. tenuifolia in rats. The composition (gkg-1 of alkaloids, cyanogenic glycosides, tannins and flavonoids were 13.3±0.1, 21.2×10-2±0.6, 1.3±0.1, 1.7±0.1 and 11.7±1.1 respectively. The proximate composition (gkg-1 of M. tenuifolia seed were crude fibre (262.3±1.2, crude protein (82.6±1.0, crude fat (349.9±1.9, ash (49.9±0.6, moisture (190.0±0.00 and carbohydrate (65.5±4.7. Analysis of the minerals content (gkg-1 yielded calcium (864.0±29.38, sodium (2752.0±140.35, iron (3.34±0.06, zinc (5.26±0.08, potassium (326.4±13.06, magnesium (342.9±13.71 and phosphorus (9.52±0.17, while vitamin analysis yielded vitamin A (10.05±0.17 iu/100 g, C (56.40±0.14 gkg-1 and E (11.71±0.87 iu /100 g, thiamine (0.11±0.01 gkg-1, niacin (0.46±0.32 gkg-1 and riboflavin (0.04±0.01 gkg-1. The results of amino acid analysis showed the total amino acid of M. tenuifolia seed was 71.78 of crude protein. The total essential amino acid with Histidine was calculated to be 29.24 of the crude protein. The antinutrient analysis of M. tenuifolia shows it contained total phenol (0.8±0.0 gkg-1, oxalates (4.09±1.17 gkg-1, phytates (0.012±0.42 gkg-1 and trypsin inhibitor (0.230±0.42 iu/g. The main fatty acids of the seed oil are linoleic acid (401.7 g kg-1, oleic acid (346.1 g kg-1 and palmitic acid (122.61 g kg-1. The LD50 of the flavonoid-rich fraction was found to be above 5000 mg kg-1 b.w. After the day 14 study, biochemical markers such as triacylglycerol, very low density lipoprotein increased significantly (p0.05 effect was observed on the blood glucose and lipid profile of wistar albino rats compared with the control. The result shows that M. tenuifolia seed is rich

  14. Nebivolol reduces central blood pressure in stage I hypertensive patients: experimental single cohort study

    Directory of Open Access Journals (Sweden)

    Renan Oliveira Vaz-de-Melo

    Full Text Available CONTEXT AND OBJECTIVES: Assessment of central blood pressure (BP has grown substantially over recent years because evidence has shown that central BP is more relevant to cardiovascular outcomes than peripheral BP. Thus, different classes of antihypertensive drugs have different effects on central BP despite similar reductions in brachial BP. The aim of this study was to investigate the effect of nebivolol, a β-blocker with vasodilator properties, on the biochemical and hemodynamic parameters of hypertensive patients.DESIGN AND SETTING: Experimental single cohort study conducted in the outpatient clinic of a university hospital.METHODS: Twenty-six patients were recruited. All of them underwent biochemical and hemodynamic evaluation (BP, heart rate (HR, central BP and augmentation index before and after 3 months of using nebivolol.RESULTS: 88.5% of the patients were male; their mean age was 49.7 ± 9.3 years and most of them were overweight (29.6 ± 3.1 kg/m2 with large abdominal waist (102.1 ± 7.2 cm. There were significant decreases in peripheral systolic BP (P = 0.0020, diastolic BP (P = 0.0049, HR (P < 0.0001 and central BP (129.9 ± 12.3 versus 122.3 ± 10.3 mmHg; P = 0.0083 after treatment, in comparison with the baseline values. There was no statistical difference in the augmentation index or in the biochemical parameters, from before to after the treatment.CONCLUSIONS: Nebivolol use seems to be associated with significant reduction of central BP in stage I hypertensive patients, in addition to reductions in brachial systolic and diastolic BP.

  15. Lowering of blood pressure by increasing hematocrit with non nitric oxide scavenging red blood cells. (United States)

    Salazar Vázquez, Beatriz Y; Cabrales, Pedro; Tsai, Amy G; Johnson, Paul C; Intaglietta, Marcos


    Isovolemic exchange transfusion of 40% of the blood volume in awake hamsters was used to replace native red blood cells (RBCs) with RBCs whose hemoglobin (Hb) was oxidized to methemoglobin (MetHb), MetRBCs. The exchange maintained constant blood volume and produced different final hematocrits (Hcts), varying from 48 to 62% Hct. Mean arterial pressure (MAP) did not change after exchange transfusion, in which 40% of the native RBCs were replaced with MetRBCs, without increasing Hct. Increasing Hct with MetRBCs lowered MAP by 12 mm Hg when Hct was increased 12% above baseline. Further increases of Hct with MetRBCs progressively returned MAP to baseline, which occurred at 62% Hct, a 30% increase in Hct from baseline. These observations show a parabolic "U" shaped distribution of MAP against the change in Hct. Cardiac index, cardiac output divided by body weight, increased between 2 and 17% above baseline for the range of Hcts tested. Peripheral vascular resistance (VR) was decreased 18% from baseline when Hct was increased 12% from baseline. VR and MAP were above baseline for increases in Hct higher than 30%. However, vascular hindrance, VR normalized by blood viscosity (which reflects the contribution of vascular geometry), was lower than baseline for all the increases in Hct tested with MetRBC, indicating prevalence of vasodilation. These suggest that acute increases in Hct with MetRBCs increase endothelium shear stress and stimulate the production of vasoactive factors (e.g., nitric oxide [NO]). When MetRBCs were compared with functional RBCs, vasodilation was augmented for MetRBCs probably due to the lower NO scavenging of MetHb. Consequently, MetRBCs increased the viscosity related hypotension range compared with functional RBCs as NO shear stress vasodilation mediated responses are greater.

  16. Blood Typing (United States)

    ... if you need repeated transfusions, as sickle cell anemia and thalassemia patients do. If blood transfusions are not closely ... the News Article Index About This Site Send Us Your Comments For ...

  17. Blood Disorders (United States)

    ... people with blood disorders. Magnitude of the Problem Complications from deep vein thrombosis (DVT) kill more people each year than breast cancer, motor vehicle accidents, and HIV combined. Sickle cell trait ...

  18. What's Blood? (United States)

    ... Rh" because scientists found it while studying Rhesus monkeys. If your blood is positive, you have this ... doctor. © 1995- The Nemours Foundation. All rights reserved. Images provided by The Nemours Foundation, iStock, Getty Images, ...

  19. Artificial blood.



    #Blood substitutes have been developed for almost a century. The various type of artificial blood was continuously available on the market. The theme of this report is to identify the best substitute in emergency situation for some patients and science students. The definition of best is given; thus, as the vital part of the report, the comparison between them is described and discussed. Modified hemoglobin, bovine-based hemoglobin and PFCs are three basic types. In terms of the perfor...

  20. Using Baseline Studies in the Investigation of Test Impact (United States)

    Wall, Dianne; Horak, Tania


    The purpose of this article is to discuss the role of "baseline studies" in investigations of test impact and to illustrate the type of thinking underlying the design and implementation of such studies by reference to a recent study relating to a high-stakes test of English language proficiency. Baseline studies are used to describe an educational…

  1. Digital Offshore Cadastre (DOC) - Pacific83 - Baseline Points (United States)

    Bureau of Ocean Energy Management, Department of the Interior — This data set contains baseline points in ESRI Arc/Info export and Arc/View shape file formats for the BOEM Pacific Region. Baseline points are used by the BOEM to...

  2. Searching for neutrino oscillation parameters in long baseline experiments

    CERN Document Server

    Vihonen, Sampsa


    Developing neutrino astronomy requires a good understanding of the neutrino oscillations mechanism. The European strategy for neutrino oscillation physics sets a high priority on future long baseline neutrino experiments with the aim to measure the intrinsic parameters that govern the neutrino oscillations. In this work we take a look at the next generation of long baseline experiments and discuss their prospects in future research.

  3. The 2014 ALMA Long Baseline Campaign : An Overview

    NARCIS (Netherlands)

    ALMA Partnership, [Unknown; Fomalont, E. B.; Vlahakis, C.; Corder, S.; Remijan, A.; Barkats, D.; Lucas, R.; Hunter, T. R.; Brogan, C. L.; Asaki, Y.; Matsushita, S.; Dent, W. R. F.; Hills, R. E.; Phillips, N.; Richards, A. M. S.; Cox, P.; Amestica, R.; Broguiere, D.; Cotton, W.; Hales, A. S.; Hiriart, R.; Hirota, A.; Hodge, J. A.; Impellizzeri, C. M. V.; Kern, J.; Kneissl, R.; Liuzzo, E.; Marcelino, N.; Marson, R.; Mignano, A.; Nakanishi, K.; Nikolic, B.; Perez, J. E.; Pérez, L. M.; Toledo, I.; Aladro, R.; Butler, B.; Cortes, J.; Cortes, P.; Dhawan, V.; Di Francesco, J.; Espada, D.; Galarza, F.; Garcia-Appadoo, D.; Guzman-Ramirez, L.; Humphreys, E. M.; Jung, T.; Kameno, S.; Laing, R. A.; Leon, S.; Mangum, J.; Marconi, G.; Nagai, H.; Nyman, L.-A.; Radiszcz, M.; Rodón, J. A.; Sawada, T.; Takahashi, S.; Tilanus, R. P. J.; van Kempen, T.; Vila Vilaro, B.; Watson, L. C.; Wiklind, T.; Gueth, F.; Tatematsu, K.; Wootten, A.; Castro-Carrizo, A.; Chapillon, E.; Dumas, G.; de Gregorio-Monsalvo, I.; Francke, H.; Gallardo, J.; Garcia, J.; Gonzalez, S.; Hibbard, J. E.; Hill, T.; Kaminski, T.; Karim, A.; Krips, M.; Kurono, Y.; Lopez, C.; Martin, S.; Maud, L.; Morales, F.; Pietu, V.; Plarre, K.; Schieven, G.; Testi, L.; Videla, L.; Villard, E.; Whyborn, N.; Alves, F.; Andreani, P.; Avison, A.; Barta, M.; Bedosti, F.; Bendo, G. J.; Bertoldi, F.; Bethermin, M.; Biggs, A.; Boissier, J.; Brand, J.; Burkutean, S.; Casasola, V.; Conway, J.; Cortese, L.; Dabrowski, B.; Davis, T. A.; Diaz Trigo, M.; Fontani, F.; Franco-Hernandez, R.; Fuller, G.; Galvan Madrid, R.; Giannetti, A.; Ginsburg, A.; Graves, S. F.; Hatziminaoglou, E.; Hogerheijde, M.; Jachym, P.; Jimenez Serra, I.; Karlicky, M.; Klaasen, P.; Kraus, M.; Kunneriath, D.; Lagos, C.; Longmore, S.; Leurini, S.; Maercker, M.; Magnelli, B.; Marti Vidal, I.; Massardi, M.; Maury, A.; Muehle, S.; Muller, S.; Muxlow, T.; O’Gorman, E.; Paladino, R.; Petry, D.; Pineda, J.; Randall, S.; Richer, J. S.; Rossetti, A.; Rushton, A.; Rygl, K.; Sanchez Monge, A.; Schaaf, R.; Schilke, P.; Stanke, T.; Schmalzl, M.; Stoehr, F.; Urban, S.; van Kampen, E.; Vlemmings, W.; Wang, K.; Wild, W.; Yang, Y.; Iguchi, S.; Hasegawa, T.; Saito, M.; Inatani, J.; Mizuno, N.; Asayama, S.; Kosugi, G.; Morita, K.-I.; Chiba, K.; Kawashima, S.; Okumura, S. K.; Ohashi, N.; Ogasawara, R.; Sakamoto, S.; Noguchi, T.; Huang, Y.-D.; Liu, S.-Y.; Kemper, F.; Koch, P. M.; Chen, M.-T.; Chikada, Y.; Hiramatsu, M.; Iono, D.; Shimojo, M.; Komugi, S.; Kim, J.; Lyo, A.-R.; Muller, E.; Herrera, C.; Miura, R. E.; Ueda, J.; Chibueze, J.; Su, Y.-N.; Trejo-Cruz, A.; Wang, K.-S.; Kiuchi, H.; Ukita, N.; Sugimoto, M.; Kawabe, R.; Hayashi, M.; Miyama, S.; Ho, P. T. P.; Kaifu, N.; Ishiguro, M.; Beasley, A. J.; Bhatnagar, S.; Braatz, J. A., III; Brisbin, D. G.; Brunetti, N.; Carilli, C.; Crossley, J. H.; D’Addario, L.; Donovan Meyer, J. L.; Emerson, D. T.; Evans, A. S.; Fisher, P.; Golap, K.; Griffith, D. M.; Hale, A. E.; Halstead, D.; Hardy, E. J.; Hatz, M. C.; Holdaway, M.; Indebetouw, R.; Jewell, P. R.; Kepley, A. A.; Kim, D.-C.; Lacy, M. D.; Leroy, A. K.; Liszt, H. S.; Lonsdale, C. J.; Matthews, B.; McKinnon, M.; Mason, B. S.; Moellenbrock, G.; Moullet, A.; Myers, S. T.; Ott, J.; Peck, A. B.; Pisano, J.; Radford, S. J. E.; Randolph, W. T.; Rao Venkata, U.; Rawlings, M. G.; Rosen, R.; Schnee, S. L.; Scott, K. S.; Sharp, N. K.; Sheth, K.; Simon, R. S.; Tsutsumi, T.; Wood, S. J.


    A major goal of the Atacama Large Millimeter/submillimeter Array (ALMA) is to make accurate images with resolutions of tens of milliarcseconds, which at submillimeter (submm) wavelengths requires baselines up to ∼15 km. To develop and test this capability, a Long Baseline Campaign (LBC) was carried

  4. Adapting the M3 Surveillance Metrics for an Unknown Baseline

    Energy Technology Data Exchange (ETDEWEB)

    Hamada, Michael Scott [Los Alamos National Lab. (LANL), Los Alamos, NM (United States); Abes, Jeff I. [Los Alamos National Lab. (LANL), Los Alamos, NM (United States); Jaramillo, Brandon Michael Lee [Los Alamos National Lab. (LANL), Los Alamos, NM (United States)


    The original M3 surveillance metrics assume that the baseline is known. In this article, adapted M3 metrics are presented when the baseline is not known and estimated by available data. Deciding on how much available data is enough is also discussed.

  5. 77 FR 26535 - Hope Gas, Inc.; Notice of Baseline Filing (United States)


    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF ENERGY Federal Energy Regulatory Commission Hope Gas, Inc.; Notice of Baseline Filing Take notice that on April 26, 2012, Hope Gas, Inc. (Hope Gas) submitted a baseline filing of their Statement of Operating Conditions...

  6. 77 FR 31841 - Hope Gas, Inc.; Notice of Baseline Filing (United States)


    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF ENERGY Federal Energy Regulatory Commission Hope Gas, Inc.; Notice of Baseline Filing Take notice that on May 16, 2012, Hope Gas, Inc. (Hope Gas) submitted a revised baseline filing of their Statement of...

  7. 饲粮蛋白脂肪比对圆斑星鲽(Verasper variegates)生长、消化酶及血清生化指标的影响%The Effects of the Ratio of Dietary Protein to Lipid on the Growth, Digestive Enzyme Activities and Blood Biochemical Parameters in Spotted Halibut,Verasper variegates

    Institute of Scientific and Technical Information of China (English)

    吕云云; 陈四清; 于朝磊; 常青; 秦搏; 王志军


    Spotted halibut,Verasper variegates has been a target model in the studies of reproductive biology and molecular biology. However, litter is known about the dietary requirements of this species. In this study, we determined the optimal ratio of protein to lipid for this fish and analyzed the combined effects on the growth,digestive enzyme activities and blood biochemical parameters inspotted halibut. Fish meal and casein were used as protein sources, and soybean oil and fish oil served as lipid sources. Nine experimental diets were formulated as combinations of three levels of crude proteins (40%, 45%, and 50%) and three levels of crude lipid (8%, 12%, and 16%), namely P40L8, P40L12, P40L16, P45L8, P45L12, P45L16, P50L8, P50L12 and P50L16. They were randomly distributed into 27 cement ponds (2 m×1 m×1 m) with 20 fish/pond for 83 d in an in-door flow-through aquarium system. The results showed that the survival rate of fish was not significantly affected by the levels of protein and lipid (P>0.05). The weight gain, specific growth rate and feed intake were significantly reduced along with the increase in the dietary lipid (P<0.05). Compared to P40L16, the weight gain in P50L8 was 33.63% higher and the specific growth rate was 31% higher. The feed efficiency was significantly boosted along with the increase in the dietary protein (P<0.05). The feed efficiency in P50L8 was 24.09% higher than P40L8, and the weight gain, specific growth rate, feed intake and feed efficiency in P50L8 were significantly higher than those in other groups. The activity of stomach protease was first increased and then decreased along with the increase in dietary protein. The protease activity in the hepatopancreas in P50L8 group was significantly higher than that in P50L12 group (P<0.05), but showed no significant differences with other groups (P>0.05). The activity of the stomach lipase was first decreased and then increased along with the increase in the dietary protein. The

  8. A dual investigation of the effect of dietary supplementation with licorice flavonoid oil on anthropometric and biochemical markers of health and adiposity

    Directory of Open Access Journals (Sweden)

    Bloomer Richard J


    Full Text Available Abstract Background Licorice flavonoid oil (LFO has been reported to minimize visceral adipose tissue gain in obese mice and to result in a decrease in body weight and body fat in humans; the effects of which may be more pronounced when administered in an overfed state. Methods We investigated the effects of LFO in two separate studies. Study 1 included a sample of overweight or grade I-II obese men and women (N = 22 who followed their usual dietary and physical activity programs. Study 2 included a sample of athletic men who followed their usual dietary and physical activity programs but consumed a daily supplemental meal (25% above daily energy requirements in an attempt to induce a state of overfeeding. In both studies, subjects were randomly assigned (double-blind to either LFO or a placebo for eight weeks, and anthropometric and multiple biochemical outcomes (e.g., markers of oxidative stress, markers of insulin sensitivity, blood lipids, etc. were obtained before and following the intervention. Results No differences of statistical significance were noted between LFO and placebo for any measured variable in Study 1 or Study 2. When investigating the percent change from baseline for data in Study 2, although not of statistical significance, subjects in the LFO condition experienced less overall fat gain, as well as attenuation in the elevation in selected blood lipids (e.g., cholesterol, LDL-C, and triglycerides. Conclusion These combined data indicate little effect of LFO supplementation within a sample of overweight/obese men and women or athletic men, with the possible exception of attenuation in body fat gain and selected components of the blood lipid panel in response to an overfeeding condition.

  9. A Program on Biochemical and Biomedical Engineering. (United States)

    San, Ka-Yiu; McIntire, Larry V.


    Presents an introduction to the Biochemical and Biomedical Engineering program at Rice University. Describes the development of the academic and enhancement programs, including organizational structure and research project titles. (YP)

  10. Blood Transfusion and Donation (United States)

    ... receiving the blood transfusion. To keep blood safe, blood banks carefully screen donated blood. The risk of catching ... one or more times before the surgery. A blood bank will store your blood for your use. NIH: ...

  11. Blood (For Parents) (United States)

    ... Old Feeding Your 1- to 2-Year-Old Blood KidsHealth > For Parents > Blood A A A What's ... about the mysterious, life-sustaining fluid called blood. Blood Basics Two types of blood vessels carry blood ...

  12. Catecholamine blood test (United States)

    Norepinephrine -- blood; Epinephrine -- blood; Adrenalin -- blood; Dopamine -- blood ... A blood sample is needed. ... the test. This is especially true if both blood and urine catecholamines are to be measured. You ...

  13. Biology of Blood (United States)

    ... here for the Professional Version Home Blood Disorders Biology of Blood Overview of Blood Resources In This ... Version. DOCTORS: Click here for the Professional Version Biology of Blood Overview of Blood Components of Blood ...

  14. Blood Clotting and Pregnancy

    Medline Plus

    Full Text Available ... Blood Basics Blood Disorders Anemia Bleeding Disorders Blood Cancers Blood Clots Blood Clotting and Pregnancy Clots and ... Increased maternal age Other medical illness (e.g., cancer, infection) back to top How are Blood Clots ...

  15. Severe hyperkalemia following blood transfusions: Is there a link? (United States)

    Rizos, Christos V; Milionis, Haralampos J; Elisaf, Moses S


    Patients with gastrointestinal bleeding often require large volume blood transfusion. Among the various side effects of blood transfusion, the increase of potassium levels is a serious one which is often overlooked. We report a case of severe hyperkalemia in a patient with gastric bleeding after large volume transfusion of packed red blood cells. The patient had hyperkalemia at baseline associated with his receiving medication as well as acute renal failure following hypovolemia. The baseline hyperkalemia was further aggravated after massive transfusions of packed red blood cells in a short period of time. The associated pathogenetic mechanisms resulting in the increase of potassium levels are presented. A number of risk factors which increase the risk of hyperkalemia after blood transfusion are discussed. Moreover, appropriate management strategies for the prevention of blood transfusion associated hyperkalemia are also presented. Physicians should always keep in mind the possibility of hyperkalemia in cases of blood transfusion. PMID:28101452

  16. Baseline Plasma C-Reactive Protein Concentrations and Motor Prognosis in Parkinson Disease.

    Directory of Open Access Journals (Sweden)

    Atsushi Umemura

    Full Text Available C-reactive protein (CRP, a blood inflammatory biomarker, is associated with the development of Alzheimer disease. In animal models of Parkinson disease (PD, systemic inflammatory stimuli can promote neuroinflammation and accelerate dopaminergic neurodegeneration. However, the association between long-term systemic inflammations and neurodegeneration has not been assessed in PD patients.To investigate the longitudinal effects of baseline CRP concentrations on motor prognosis in PD.Retrospective analysis of 375 patients (mean age, 69.3 years; mean PD duration, 6.6 years. Plasma concentrations of high-sensitivity CRP were measured in the absence of infections, and the Unified Parkinson's Disease Rating Scale Part III (UPDRS-III scores were measured at five follow-up intervals (Days 1-90, 91-270, 271-450, 451-630, and 631-900.Change of UPDRS-III scores from baseline to each of the five follow-up periods.Change in UPDRS-III scores was significantly greater in PD patients with CRP concentrations ≥0.7 mg/L than in those with CRP concentrations <0.7 mg/L, as determined by a generalized estimation equation model (P = 0.021 for the entire follow-up period and by a generalized regression model (P = 0.030 for the last follow-up interval (Days 631-900. The regression coefficients of baseline CRP for the two periods were 1.41 (95% confidence interval [CI] 0.21-2.61 and 2.62 (95% CI 0.25-4.98, respectively, after adjusting for sex, age, baseline UPDRS-III score, dementia, and incremental L-dopa equivalent dose.Baseline plasma CRP levels were associated with motor deterioration and predicted motor prognosis in patients with PD. These associations were independent of sex, age, PD severity, dementia, and anti-Parkinsonian agents, suggesting that subclinical systemic inflammations could accelerate neurodegeneration in PD.

  17. Implementation of revised strategy of filaria control-baseline clinico-parasitological survey. (United States)

    Patel, S; Rai, R N; Mishra, R N


    A clinico-epidemiological study of filariasis was carried out in Varanasi District in October and November, 1997 to generate baseline data for assessing the impact of Mass Drug Administration (MDA) in the district. Disease rate was found to be 6.6% (9.9% in males and 3.0% in females) and microfilaria rate was 5.3% (5.2% in males and 5.5% in females). Mean microfilaria density was found to be 9.86 per 20 blood. Genital manifestations (77.5%) outnumbered all other forms of clinical manifestations. Vector infectivity rate was found to be 0.93%.

  18. Managing your blood sugar (United States)

    Hyperglycemia - control; Hypoglycemia - control; Diabetes - blood sugar control; Blood glucose - managing ... Know how to: Recognize and treat low blood sugar (hypoglycemia) Recognize and treat high blood sugar (hyperglycemia) ...

  19. Effects of a low-fat diet with antioxidant supplementation on biochemical markers of multiple sclerosis long-term care residents

    Directory of Open Access Journals (Sweden)

    Elba Mauriz


    Full Text Available Introduction: Multiple sclerosis (MS treatment options are primarily limited to immunomodulatory therapies in MS non-progressive forms. Nutrition intervention studies suggest that diet may be considered as a complementary treatment to control disease progression. Therefore, dietary intervention may help to improve wellness and ameliorate symptoms of MS patients. Objectives: To assess the effect of a low-fat diet with antioxidant supplementation on biochemical markers of institutionalized patients with progressive forms of multiple sclerosis. Methods: A randomized prospective placebo-controlled study involving 9 participants, 5 of them assigned to the intervention group (low-fat diet and antioxidant supplementation and the other 4 to the placebo group (low-fat diet. The effect of the dietary intervention, involving diet modification and antioxidant supplementation, was examined for 42 days by measuring anthropometric, biochemical parameters and oxidative stress markers in blood at baseline (day 0, intermediate (day 15 and end (day 42 stages of the treatment. Results: The intervention group obtained C reactive protein levels significantly lower than those observed in the corresponding placebo group at the end of the study. Oxidative stress and inflammatory markers isoprostane 8-iso-PGF2α and interleukine IL-6 values also diminished after dietary intervention in the intervention group. Catalase activity increased significantly in the intervention group prior antioxidant supplementation. No significant differences were observed in other oxida-tive stress markers. Conclusions: The results suggest that diet and dietary supplements are involved in cell metabolism modulation and MS-related inflammatory processes. Consequently, low fat diets and antioxidant supplements may be used as complementary therapies for treatment of multiple sclerosis.

  20. Physiological and Biochemical Indexes of Tibetan Sheep at Different Months of Age

    Institute of Scientific and Technical Information of China (English)

    Zhou Mingliang; Jiang Shihai; Chen Minghua; Yang Pinggui; Wu Weisheng; Xie Rongqing


    To study the changes in physiological and biochemical indexes of Tibetan sheep from the plateau area at different months of age,we measured the physiological and biochemical indexes of Tibetan sheep reared in Hongyuan County of Sichuan Province according to their growth stages. The results showed that the respiration,body temperature and heart rate of Tibetan sheep at 3- 24 months of age were( 24. 60 ± 6. 33)-( 50. 30 ± 21. 18) times / min,( 39. 14 ± 0. 28) ℃-( 39. 64 ± 0. 17) ℃ and( 91. 00 ± 13. 27)-( 112. 00 ± 8. 69) times/min,respectively. The blood physiological indexes of Tibetan sheep including white blood cell count( WBC),hematocrit( HCT) and red blood cell distribution width coefficient of variation( RDW- CV) at 3,6,15 and 18 months of age were relatively lower than those at 9,12,21 and 24 months of age. The blood physiological indexes including red blood cell count( RBC),mean corpuscular capacity( MCV),mean corpuscular hemoglobin concentration( MCHC) and mean corpuscular hemoglobin( MCH) at 3,6,15 and 18 months of age were relatively higher than those at 9,12,21 and 24 months of age,while hemoglobin( HGB) and platelet count( PLT) did not vary with months of age. For blood biochemical indexes,alanine aminotransferase( ALT),albumin( ALB),globulin( GLO) and total cholesterol( CHOL) reached the maximum values at 21 months of age; alkaline phosphatase( ALKP),lactate dehydrogenase( LDH),blood glucose( GLU) and pseudocholinesterase( PCHE) reached the maximum values at 3 months of age;and total calcium( CA),total protein( TP),aspartate aminotransferase( AST) reached the maximum values at 9,18 and 24 months of age,respectively.

  1. The Harbin Cohort Study on Diet, Nutrition and Chronic Non-communicable Diseases: study design and baseline characteristics.

    Directory of Open Access Journals (Sweden)

    Lixin Na

    Full Text Available Diet and nutrition have been reported to be associated with many common chronic diseases and blood-based assessment would be vital to investigate the association and mechanism, however, blood-based prospective studies are limited. The Harbin Cohort Study on Diet, Nutrition and Chronic Non-communicable Diseases was set up in 2010. From 2010 to 2012, 9,734 participants completed the baseline survey, including demographic characteristics, dietary intake, lifestyles and physical condition, and anthropometrics. A re-survey on 490 randomly selected participants was done by using the same methods which were employed in the baseline survey. For all participants, the mean age was 50 years and 36% of them were men. Approximately 99.4 % of cohort members donated blood samples. The mean total energy intake was 2671.7 kcal/day in men and 2245.9 kcal/day in women, the mean body mass index was 25.7 kg/m2 in men and 24.6 kg/m2 in women, with 18.4% being obese (≥ 28 kg/m2, 12.7% being diabetic, and 29.5% being hypertensive. A good agreement was obtained for the physical measurements between the baseline survey and re-survey. The resources from the cohort and its fasting and postprandial blood samples collected both at baseline and in each follow-up will be valuable and powerful in investigating relationship between diet, nutrition and chronic diseases and discovering novel blood biomarkers and the metabolism of these biomarkers related to chronic diseases.

  2. The Research on Biochemical Indexes of Athletes after Taking Nutritional Supplements during Winter Training

    Directory of Open Access Journals (Sweden)

    Liping Pan


    Full Text Available The paper takes hemoglobin and blood urea as the indexes of biochemical monitoring and accordingly supplying sports nutritional supplements into them at the same time, the effect on exercise capacity and the changes in biochemical indicators in different training stages will be observed in this research. The quality of winter training determines the motion states of athletes in the next year. However, the poor weather condition and the large amount of training will lead to the accumulation of fatigue, affecting the training status of athletes seriously. Therefore, it is essential to monitor their biochemical indicators and supply them with special nutrition. In order to ensure the completion of a large load of training for athletes, it is far enough to supplement the nutrients just by food, some special powerful nutrition are also needed in a scientific way.

  3. Histological and Biochemical Evaluation of the Kidney following Chronic Consumption of Hibiscus sabdariffa

    Directory of Open Access Journals (Sweden)

    U. U. Ukoha


    Full Text Available Hibiscus sabdariffa L. has been used traditionally as herbal medicine and has been documented to have a broad range of therapeutic effects. The present study aimed to investigate the effects of chronic administration of aqueous extract of flowers of Hibiscus sabdariffa on the histology of the kidney and some biochemical indices of renal function in male Wistar rats. Twenty (20 Wistar rats were randomly divided into four (4 groups of five rats each. The extract was administered orally in doses 200, 500, and 800 mg/kg body weight for 21 days. The kidney was harvested and processed histologically and blood samples were taken for biochemical assays. The histological results showed dose dependent pathological states and the biochemical analysis revealed a dose dependant variation in renal indices. These results suggest that chronic administration of aqueous extract of Hibiscus sabdariffa may be toxic to the kidney.

  4. The MINK methodology: background and baseline. [USA - Midwest Region

    Energy Technology Data Exchange (ETDEWEB)

    Rosenberg, N.J.; Crosson, P.R.; Frederick, K.D.; Easterling, W.E. III; McKenney, M.S.; Bowes, M.D.; Sedjo, R.A.; Darmstadter, J.; Katz, L.A.; Lemon, K.M. (Resources for the Future, Washington, DC (United States))


    A four step methodology has been developed for study of the regional impacts of climate change and the possible responses thereto. First the region's climate sensitive sectors and total economy are described (Task A, current baseline). Next a scenario of climate change is imposed on the current baseline (Task B, current baseline with climate change). A new baseline describing the climate sensitive sectors and total regional economy is projected for some time in the future (Task C, future baseline, year 2030) in the absence of climate change. Finally, the climate change scenario is reimposed on the future baseline (Task D, future baseline with climate change). Impacts of the climate change scenario on the current and future regional economies are determined by means of simulation models and other appropriate techniques. These techniques are also used to assess the impacts of an elevated CO[sub 2] concentration (450 ppm) and of various forms of adjustments and adaptations. The region chosen for the first test of the methodology is composed of the four U.S. states of Missouri, Iowa, Nebraska and Kansas. The climate change scenario is the actual weather of the 1930s decade in the MINK region. 'Current' climate is the actual weather of the period 1951-1980. 20 refs., 3 figs., 2 tabs.

  5. Hyperhomocysteinemia decreases bone blood flow

    Directory of Open Access Journals (Sweden)

    Neetu T


    Full Text Available Neetu Tyagi*, Thomas P Vacek*, John T Fleming, Jonathan C Vacek, Suresh C TyagiDepartment of Physiology and Biophysics, School of Medicine, University of Louisville, Louisville, KY, USA *These authors have equal authorshipAbstract: Elevated plasma levels of homocysteine (Hcy, known as hyperhomocysteinemia (HHcy, are associated with osteoporosis. A decrease in bone blood flow is a potential cause of compromised bone mechanical properties. Therefore, we hypothesized that HHcy decreases bone blood flow and biomechanical properties. To test this hypothesis, male Sprague–Dawley rats were treated with Hcy (0.67 g/L in drinking water for 8 weeks. Age-matched rats served as controls. At the end of the treatment period, the rats were anesthetized. Blood samples were collected from experimental or control rats. Biochemical turnover markers (body weight, Hcy, vitamin B12, and folate were measured. Systolic blood pressure was measured from the right carotid artery. Tibia blood flow was measured by laser Doppler flow probe. The results indicated that Hcy levels were significantly higher in the Hcy-treated group than in control rats, whereas vitamin B12 levels were lower in the Hcy-treated group compared with control rats. There was no significant difference in folate concentration and blood pressure in Hcy-treated versus control rats. The tibial blood flow index of the control group was significantly higher (0.78 ± 0.09 flow unit compared with the Hcy-treated group (0.51 ± 0.09. The tibial mass was 1.1 ± 0.1 g in the control group and 0.9 ± 0.1 in the Hcy-treated group. The tibia bone density was unchanged in Hcy-treated rats. These results suggest that Hcy causes a reduction in bone blood flow, which contributes to compromised bone biomechanical properties.Keywords: homocysteine, tibia, bone density

  6. Neutrino oscillations: what is magic about the "magic" baseline?

    CERN Document Server

    Smirnov, A Yu


    Physics interpretation of the ``magic'' baseline that can play important role in future oscillation experiments is given. The ``magic'' baseline coincides with the refraction length, $l_0$. The latter, in turn, approximately equals the oscillation length in matter at high energies. Therefore at the baseline $L = l_0$ the oscillation phase is $2\\pi$, and consequently, the ``solar'' amplitude of oscillations driven by the mixing angle $\\theta_{12}$ and mass splitting $\\Delta m^2_{21}$ vanishes. As a result, in the lowest order (i) the interference of amplitudes in the $\

  7. Trinocular Stereo Matching Based on Correlations Between Baselines and Disparities

    Institute of Scientific and Technical Information of China (English)

    GUANYepeng; GUWeikang


    Gray correlation technique is utilized to take the multi-peak feature points with gray correlation coefficients less than a certain range of maximal correlation coefficient as a potential candidate matching set. There exists the maximal correlation between the correct disparities and their corresponding baselines. A trinocular stereo matching algorithm is proposed based on correlations between the baselines and disparities. After computing the correlations between the baselines and disparities, the unique matches can be determined by maximal correlation coefficient. It is proved that the algorithm proposed is valid and credible by 3-D reconstruction on two pairs of actual natural stereo images.

  8. Biochemical characterization of exercise-trained porcine myocardium. (United States)

    Laughlin, M H; Hale, C C; Novela, L; Gute, D; Hamilton, N; Ianuzzo, C D


    The purpose of this study was to determine whether cardiac biochemical adaptations are induced by chronic exercise training (ET) of miniature swine. Female Yucatan miniature swine were trained on a treadmill or were cage confined (C) for 16-22 wk. After training, the ET pigs had increased exercise tolerance, lower heart rates during exercise at submaximal intensities, moderate cardiac hypertrophy, increased coronary blood flow capacity, and increased oxidative capacity of skeletal muscle. Myosin from both the C and ET hearts was 100% of the V3 isozyme, and there were no differences between the myosin adenosine triphosphatase (ATPase) or myofibrillar ATPase activities of C and ET hearts. Also, the sarcoplasmic reticulum Ca(2+)-ATPase activity and Na(+)-Ca2+ exchange activity of sarcolemmal vesicles were the same in cardiac muscle of C and ET hearts. Finally, the glycolytic and oxidative capacity of ET cardiac muscle was not different from control, since phosphofructokinase, citrate synthase, and 3-hydroxyacyl-CoA dehydrogenase activities were the same in cardiac tissue from ET and C pigs. We conclude that endurance exercise training does not provide sufficient stress on the heart of a large mammal to induce changes in any of the three major cardiac biochemical systems of the porcine myocardium: the contractile system, the Ca2+ regulatory systems, or the metabolic system.

  9. Recommended Nordic paediatric reference intervals for 21 common biochemical properties

    DEFF Research Database (Denmark)

    Hilsted, Linda; Rustad, Pål; Aksglæde, Lise;


    Abstract Paediatric reference intervals based on samples from healthy children are difficult to establish and consequently data are often from hospitalized children. Furthermore, biases may present in published data due to differences in the analytical methods employed. Blood samples from 1429...... healthy Danish children were collected for establishing reference intervals for 21 common biochemical properties (Alanine transaminase, Albumin, Alkaline phosphatase, Aspartate transaminase, Bilirubin, Calcium, Cholesterol, Creatinine, Creatine kinase, HDL-Cholesterol, Iron, Lactate dehydrogenase, LDL...... values of X for the properties and statistical calculations carried out as performed in the NORIP study. Thus commutable (regarding analytical method) reference intervals for 20 properties were established and for LDL-Cholesterol reference intervals were reported for the specific analytical method...

  10. Prediction of the preeclampsia: a view of biochemical markers

    Directory of Open Access Journals (Sweden)

    Mehmet Sühha Bostancı


    Full Text Available Preeclampsia is a diverse, multiorgan group of related disease processes that occurs in up to 5%-8% of pregnancies after 20 weeks’ gestation and it is one of the leading causes of maternal and fetal morbidity and mortality. Many molecular mechanisms are contributed to the pathogenesis of preeclampsia. Although it is unknown whether the mechanisms act independently or have synergistic effects. This review describes review of primary papers investigating blood based biomarker such as PAP-A, Inhibin A, sFlt1, and PP13 in general and first trimester biochemical markers and combinations of them specifically for preeclampsia.

  11. Biochemical composition of the alligator pipefish, Syngnathoides biaculeatus (Bloch, 1785) (United States)

    Sanaye, Sushant Vilas; Pawar, Ashwini Pandurang; Rivonker, Chandrasheker Umanath; Sreepada, Rayadurga Anantha; Ansari, Zakir Ali; Ram, Anirudh


    Considering the economic importance in traditional Chinese medicine (TCM) and lack of baseline information, we evaluated the proximate composition, fatty acid and amino acid profiles, trace element content and C:N ratio in the alligator pipefish, Syngnathoides biaculeatus. Amongst proximate principals, a crude protein formed the major biochemical component ((58.9±2.2)% dry weight). Mean percent concentrations (dry weight) of other components such as a total lipid (TL), ash and nitrogen-free extract measured were, (1.8±0.2)%, (19.2±2.2)% and (20.1±0.45)%, respectively. The fatty acid profile revealed the presence of 27 saturated fatty acids (SFA) with 13 straight-chained and 14 branched-chained, 28 unsaturated fatty acids (UFA) with 14 monounsaturated and 14 polyunsaturated and nine other minor fatty acids. Mean percent contributions of total SFAs and UFAs to TL were found to be (55.41±0.24)% and (44.05±0.25)%, respectively. Altogether, 16 different amino acids with an equal number of essential (EAA) and non-essential (NAA) ones were identified. Percent contributions by EAA and NAA to the total amino acid content were 38.11% and 61.89%, respectively. Trace metal concentrations in S. biaculeatus were generally low and their distribution followed the order, Mg>Fe>Zn>Mn>Cu>Cr>Ni>Hg>Co. The C:N ratio was (4.37±0.04)%. The profile of major biochemical constituents in alligator pipefish, S. biaculeatus revealed its potential use in TCM as well as a nutritional diet for human consumption. The results of the study would also form the basis for formulation and optimization of diets for the culture of S. biaculeatus.

  12. Clinical and biochemical significance of toxin production by Aeromonas hydrophila. (United States)

    Kindschuh, M; Pickering, L K; Cleary, T G; Ruiz-Palacios, G


    Production of cytotoxin and enterotoxin by Aeromonas strains obtained from stools of 50 children in Mexico and Texas and from blood of 9 children with sepsis was determined. Results were correlated with clinical features of infected children as well as with biochemical traits of Aeromonas strains. Cytotoxin was produced by 40 of 42 Aeromonas strains (95%) isolated from stools of children with diarrhea, by all 8 isolates from stools of well children, and by all 9 isolates from children with sepsis. There was no difference in the quantities (amount of cytotoxin per milligram of protein required to kill 50% of the cells) of cytotoxin produced and in clinical manifestations among the groups. None of the isolates produced a toxin that could be neutralized by antiserum raised against Shiga toxin produced by Shigella dysenteriae 1 60R. Heat-labile-like enterotoxin (LT) was produced by 26 of 42 stool isolates (62%), while only 1 of the 42 isolates (2%) produced enterotoxinlike activity in suckling mice; 65% of the cytotoxin-producing strains also produced an LT-like material. All strains from blood produced LT-like material, and 2 of 6 (33%) produced activity in suckling mice. All strains produced hemolysin; 37 of 57 (65%) were Voges-Proskauer positive; 27 of 57 (47%) were lysine decarboxylase positive by API 20E strips, none were positive for lysine decarboxylose production by lysin-iron agar slants at 24 h, but 17 of 54 (31%) were positive at 48 h. There was no correlation between biochemical reactions and enterotoxin or cytotoxin production. There appears to be no correlation between toxin production by Aeromonas spp. and gastroenteritis.

  13. 40 CFR 158.2010 - Biochemical pesticides data requirements. (United States)


    ... 40 Protection of Environment 23 2010-07-01 2010-07-01 false Biochemical pesticides data...) PESTICIDE PROGRAMS DATA REQUIREMENTS FOR PESTICIDES Biochemical Pesticides § 158.2010 Biochemical pesticides... required to support registration of biochemical pesticides. Sections 158.2080 through 158.2084 identify...

  14. Nonlinear biochemical signal processing via noise propagation. (United States)

    Kim, Kyung Hyuk; Qian, Hong; Sauro, Herbert M


    Single-cell studies often show significant phenotypic variability due to the stochastic nature of intra-cellular biochemical reactions. When the numbers of molecules, e.g., transcription factors and regulatory enzymes, are in low abundance, fluctuations in biochemical activities become significant and such "noise" can propagate through regulatory cascades in terms of biochemical reaction networks. Here we develop an intuitive, yet fully quantitative method for analyzing how noise affects cellular phenotypes based on identifying a system's nonlinearities and noise propagations. We observe that such noise can simultaneously enhance sensitivities in one behavioral region while reducing sensitivities in another. Employing this novel phenomenon we designed three biochemical signal processing modules: (a) A gene regulatory network that acts as a concentration detector with both enhanced amplitude and sensitivity. (b) A non-cooperative positive feedback system, with a graded dose-response in the deterministic case, that serves as a bistable switch due to noise-induced ultra-sensitivity. (c) A noise-induced linear amplifier for gene regulation that requires no feedback. The methods developed in the present work allow one to understand and engineer nonlinear biochemical signal processors based on fluctuation-induced phenotypes.

  15. Blood Basics (United States)

    ... of ASH ASH Meeting on Hematologic Malignancies Consultative Hematology Course ASH Meeting on Lymphoma Biology ASH Workshop on Genome Editing Publications Blood The Hematologist ASH Clinical News ASH Self-Assessment Program Hematology , ASH Education Program About Awards Membership ASH Foundation ...

  16. Blood Clots (United States)

    ... of ASH ASH Meeting on Hematologic Malignancies Consultative Hematology Course ASH Meeting on Lymphoma Biology ASH Workshop on Genome Editing Publications Blood The Hematologist ASH Clinical News ASH Self-Assessment Program Hematology , ASH Education Program About Awards Membership ASH Foundation ...

  17. Baseline inventory data recommendations for National Wildlife Refuges (United States)

    US Fish and Wildlife Service, Department of the Interior — The Baseline Inventory Team recommends that each refuge have available abiotic “data layers” for topography, aerial photography, hydrography, soils, boundaries, and...

  18. Baseline inventory data users guide to abiotic GIS layers (United States)

    US Fish and Wildlife Service, Department of the Interior — The Baseline Inventory Team was chartered by the Fulfilling the Promises Implementation Team to recommend minimum abiotic and biotic inventories for the National...

  19. Sterile Neutrino Fits to Short-Baseline Neutrino Oscillation Measurements

    Directory of Open Access Journals (Sweden)

    J. M. Conrad


    (3 + 2 and (3 + 3 fits, rather than (3 + 1 fits, for future neutrino oscillation phenomenology. These results motivate the pursuit of further short-baseline experiments, such as those reviewed in this paper.

  20. Baseline assessment of fish communities of the Flower Garden Banks (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The work developed baseline information on fish and benthic communities within the Flower Garden Banks National Marine Sanctuary (FGBNMS). Surveys employed diving,...

  1. Baseline vegetation mapping : Fort Niobrara National Wildlife Refuge (United States)

    US Fish and Wildlife Service, Department of the Interior — Final report for the baseline vegetation mapping project on Fort Niobrara National Wildlife Refuge. This project aims to create a vegetation map showing the...

  2. Butler Hollow Glades : Baseline assessment and vegetation monitoring establishment (United States)

    US Fish and Wildlife Service, Department of the Interior — Several sampling and documentation protocols were implemented to establish baseline vegetation data. These data will provide a comparison point for future...

  3. 40 CFR 80.93 - Individual baseline submission and approval. (United States)


    ...: (i) Refinery block flow diagram, showing principal refining units; (ii) Principal refining unit... parameter for which a baseline value is required, per § 80.91: (i) Narrative of the development of...

  4. Information architecture. Volume 2, Part 1: Baseline analysis summary

    Energy Technology Data Exchange (ETDEWEB)



    The Department of Energy (DOE) Information Architecture, Volume 2, Baseline Analysis, is a collaborative and logical next-step effort in the processes required to produce a Departmentwide information architecture. The baseline analysis serves a diverse audience of program management and technical personnel and provides an organized way to examine the Department`s existing or de facto information architecture. A companion document to Volume 1, The Foundations, it furnishes the rationale for establishing a Departmentwide information architecture. This volume, consisting of the Baseline Analysis Summary (part 1), Baseline Analysis (part 2), and Reference Data (part 3), is of interest to readers who wish to understand how the Department`s current information architecture technologies are employed. The analysis identifies how and where current technologies support business areas, programs, sites, and corporate systems.

  5. Seier NWR second year baseline CCP preparation surveys (United States)

    US Fish and Wildlife Service, Department of the Interior — Final report for a project to collect baseline biological information on John W. And Louise Seier National Wildlife Refuge, to aid in preparation of 2014...

  6. LBCS: The LOFAR Long-Baseline Calibrator Survey (United States)

    Jackson, N.; Tagore, A.; Deller, A.; Moldón, J.; Varenius, E.; Morabito, L.; Wucknitz, O.; Carozzi, T.; Conway, J.; Drabent, A.; Kapinska, A.; Orrù, E.; Brentjens, M.; Blaauw, R.; Kuper, G.; Sluman, J.; Schaap, J.; Vermaas, N.; Iacobelli, M.; Cerrigone, L.; Shulevski, A.; ter Veen, S.; Fallows, R.; Pizzo, R.; Sipior, M.; Anderson, J.; Avruch, I. M.; Bell, M. E.; van Bemmel, I.; Bentum, M. J.; Best, P.; Bonafede, A.; Breitling, F.; Broderick, J. W.; Brouw, W. N.; Brüggen, M.; Ciardi, B.; Corstanje, A.; de Gasperin, F.; de Geus, E.; Eislöffel, J.; Engels, D.; Falcke, H.; Garrett, M. A.; Grießmeier, J. M.; Gunst, A. W.; van Haarlem, M. P.; Heald, G.; Hoeft, M.; Hörandel, J.; Horneffer, A.; Intema, H.; Juette, E.; Kuniyoshi, M.; van Leeuwen, J.; Loose, G. M.; Maat, P.; McFadden, R.; McKay-Bukowski, D.; McKean, J. P.; Mulcahy, D. D.; Munk, H.; Pandey-Pommier, M.; Polatidis, A. G.; Reich, W.; Röttgering, H. J. A.; Rowlinson, A.; Scaife, A. M. M.; Schwarz, D. J.; Steinmetz, M.; Swinbank, J.; Thoudam, S.; Toribio, M. C.; Vermeulen, R.; Vocks, C.; van Weeren, R. J.; Wise, M. W.; Yatawatta, S.; Zarka, P.


    We outline the LOFAR Long-Baseline Calibrator Survey (LBCS), whose aim is to identify sources suitable for calibrating the highest-resolution observations made with the International LOFAR Telescope, which include baselines >1000 km. Suitable sources must contain significant correlated flux density (≳ 50 - 100 mJy) at frequencies around 110-190 MHz on scales of a few hundred milliarcseconds. At least for the 200-300-km international baselines, we find around 1 suitable calibrator source per square degree over a large part of the northern sky, in agreement with previous work. This should allow a randomly selected target to be successfully phase calibrated on the international baselines in over 50% of cases. Products of the survey include calibrator source lists and fringe-rate and delay maps of wide areas - typically a few degrees - around each source. The density of sources with significant correlated flux declines noticeably with baseline length over the range 200-600 km, with good calibrators on the longest baselines appearing only at the rate of 0.5 per sq. deg. Coherence times decrease from 1-3 min on 200-km baselines to about 1 min on 600-km baselines, suggesting that ionospheric phase variations contain components with scales of a few hundred kilometres. The longest median coherence time, at just over 3 min, is seen on the DE609 baseline, which at 227 km is close to being the shortest. We see median coherence times of between 80 and 110 s on the four longest baselines (580-600 km), and about 2 min for the other baselines. The success of phase transfer from calibrator to target is shown to be influenced by distance, in a manner that suggests a coherence patch at 150-MHz of the order of 1 deg. Although source structures cannot be measured in these observations, we deduce that phase transfer is affected if the calibrator source structure is not known. We give suggestions for calibration strategies and choice of calibrator sources, and describe the access to

  7. Haematological and biochemical changes in dogs naturally infected with Angiostrongylus vasorum before and after treatment

    DEFF Research Database (Denmark)

    Willesen, Jakob; Jensen, Asger Lundorff; Kristensen, Annemarie Thuri


    Haematological and biochemical parameters were studied prospectively in 48 dogs naturally infected with Angiostrongylus vasorum in a primary care setting. Samples for analysis were obtained when treatment was started and 42 days afterwards. Prior to treatment, 21% of affected dogs exhibited...... eosinophilia, whereas increased total white blood cell (WBC) counts and neutrophilia were observed in only 4.2%. WBC counts and concentrations of neutrophils, eosinophils and monocytes decreased significantly from days 0 to 42, indicating that, even in dogs without elevated absolute blood values, a low grade...... inflammatory response may be present in dogs with A. vasorum infection. Biochemical changes (especially an increase in serum globulins and a decrease in serum fructosamine) were in agreement with the findings of other studies. The results show that the diagnosis of canine angiostrongylosis should...

  8. Fusion of a Variable Baseline System and a Range Finder

    Directory of Open Access Journals (Sweden)

    Rafael Arnay


    Full Text Available One of the greatest difficulties in stereo vision is the appearance of ambiguities when matching similar points from different images. In this article we analyze the effectiveness of using a fusion of multiple baselines and a range finder from a theoretical point of view, focusing on the results of using both prismatic and rotational articulations for baseline generation, and offer a practical case to prove its efficiency on an autonomous vehicle.

  9. Effectiveness of the new polyfunctional infusion solution of blood substitutes on the activity of lipid peroxidation and antioxidant protection of heart in acute fatal blood loss

    Directory of Open Access Journals (Sweden)

    Umid Ruziev


    Conclusions: The infusion of a new multifunctional blood substitute during acute fatal blood loss leads to a more effective delay of LPO processes and restoration of AOS in heart, in comparison with the use of reosorbilact. The use of a new multifunctional blood substitute during acute lethal hemorrhage in rats, compared with infusion of reosorbilact, leads to a more pronounced recovery of hemodynamic parameters, biochemical parameters of blood and ABS.

  10. Assessment of baseline hemodynamic parameters within infarct progression areas in acute stroke patients using perfusion-weighted MRI

    Energy Technology Data Exchange (ETDEWEB)

    Ritzenthaler, Thomas; Cho, Tae-Hee; Derex, Laurent; Nighoghossian, Norbert [Hospices Civils de Lyon, Cerebrovascular Unit, Hopital Neurologique Pierre Wertheimer, Bron (France); Claude Bernard Lyon 1 University, Creatis-LRMN, UMR 5520-Inserm 630, Lyon (France); Wiart, Marlene; Berthezene, Yves [Claude Bernard Lyon 1 University, Creatis-LRMN, UMR 5520-Inserm 630, Lyon (France); Berthiller, Julien [Hospices Civils de Lyon, Pole Information Medicale Evaluation Recherche, Lyon (France); Universite Lyon 1, Lyon (France); Oestergaard, Leif [University of Aarhus, Center of Functionally Integrative Neuroscience, Aarhus University Hospital, Aarhus (Denmark); Hermier, Marc [Hospices Civils de Lyon, Neuroradiology Department, Hopital Neurologique Pierre Wertheimer, Lyon (France)


    The value of perfusion MRI for identifying the tissue at risk has been questioned. Our objective was to assess baseline perfusion-weighted imaging parameters within infarct progression areas. Patients with anterior circulation stroke without early reperfusion were included from a prospective MRI database. Sequential MRI examinations were performed on admission, 2-3 h (H2), 2-3 days (D2), and between 15 and 30 days after the initial MRI. Maps of baseline time-to-peak (TTP), mean transit time (MTT), cerebral blood volume (CBV), and cerebral blood flow (CBF) were calculated. Lesion extension areas were defined as pixels showing de novo lesions between each MRI and were generated by subtracting successive lesion masks: V{sub 0}, baseline diffusion-weighted imaging (DWI) lesion; V{sub 1}, lesion extension between baseline and H2 DWI; V{sub 2}, lesion extension from H2 to D2 DWI; and V{sub 3}, lesion extension from D2 DWI to final FLAIR. Repeated measures analysis was used to compare hemodynamic parameters within the baseline diffusion lesion and subsequent lesion extension areas. Thirty-two patients were included. Baseline perfusion parameters were significantly more impaired within the acute DWI lesion compared to lesion extension areas (TTP, p<0.0001; MTT, p<0.0001; CBF p<0.0001; CBV, p<0.0001). A significant decrease in MTT (p = 0.01) and TTP (p = 0.01) was found within successive lesion growth areas. A decreasing gradient of severity for TTP and MTT was observed within successive infarct growth areas. (orig.)

  11. MALDI-TOF Baseline Drift Removal Using Stochastic Bernstein Approximation

    Directory of Open Access Journals (Sweden)

    Howard Daniel


    Full Text Available Stochastic Bernstein (SB approximation can tackle the problem of baseline drift correction of instrumentation data. This is demonstrated for spectral data: matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF data. Two SB schemes for removing the baseline drift are presented: iterative and direct. Following an explanation of the origin of the MALDI-TOF baseline drift that sheds light on the inherent difficulty of its removal by chemical means, SB baseline drift removal is illustrated for both proteomics and genomics MALDI-TOF data sets. SB is an elegant signal processing method to obtain a numerically straightforward baseline shift removal method as it includes a free parameter that can be optimized for different baseline drift removal applications. Therefore, research that determines putative biomarkers from the spectral data might benefit from a sensitivity analysis to the underlying spectral measurement that is made possible by varying the SB free parameter. This can be manually tuned (for constant or tuned with evolutionary computation (for .


    Energy Technology Data Exchange (ETDEWEB)

    Partnership, ALMA [Astrophysics Research Institute, Liverpool John Moores University, IC2, Liverpool Science Park, 146 Brownlow Hill, Liverpool L3 5RF (United Kingdom); Fomalont, E. B.; Vlahakis, C.; Corder, S.; Remijan, A.; Barkats, D.; Dent, W. R. F.; Phillips, N.; Cox, P.; Hales, A. S. [Joint ALMA Observatory, Alonso de Córdova 3107, Vitacura, Santiago (Chile); Lucas, R. [Institut de Planétologie et d’Astrophysique de Grenoble (UMR 5274), BP 53, F-38041 Grenoble Cedex 9 (France); Hunter, T. R.; Brogan, C. L.; Amestica, R.; Cotton, W. [National Radio Astronomy Observatory, 520 Edgemont Road, Charlottesville, VA 22903 (United States); Asaki, Y. [National Astronomical Observatory of Japan, 2-21-1 Osawa, Mitaka, Tokyo 181-8588 (Japan); Matsushita, S. [Institute of Astronomy and Astrophysics, Academia Sinica, P.O. Box 23-141, Taipei 106, Taiwan (China); Hills, R. E. [Astrophysics Group, Cavendish Laboratory, JJ Thomson Avenue, Cambridge CB3 0HE (United Kingdom); Richards, A. M. S. [Jodrell Bank Centre for Astrophysics, School of Physics and Astronomy, University of Manchester, Oxford Road, Manchester M13 9PL (United Kingdom); Broguiere, D., E-mail: [Institut de Radioastronomie Millime´trique (IRAM), 300 rue de la Piscine, Domaine Universitaire, F-38406 Saint Martin d’Hères (France); and others


    A major goal of the Atacama Large Millimeter/submillimeter Array (ALMA) is to make accurate images with resolutions of tens of milliarcseconds, which at submillimeter (submm) wavelengths requires baselines up to ∼15 km. To develop and test this capability, a Long Baseline Campaign (LBC) was carried out from 2014 September to late November, culminating in end-to-end observations, calibrations, and imaging of selected Science Verification (SV) targets. This paper presents an overview of the campaign and its main results, including an investigation of the short-term coherence properties and systematic phase errors over the long baselines at the ALMA site, a summary of the SV targets and observations, and recommendations for science observing strategies at long baselines. Deep ALMA images of the quasar 3C 138 at 97 and 241 GHz are also compared to VLA 43 GHz results, demonstrating an agreement at a level of a few percent. As a result of the extensive program of LBC testing, the highly successful SV imaging at long baselines achieved angular resolutions as fine as 19 mas at ∼350 GHz. Observing with ALMA on baselines of up to 15 km is now possible, and opens up new parameter space for submm astronomy.

  13. Baseline omega-3 index correlates with aggressive and attention deficit disorder behaviours in adult prisoners.

    Directory of Open Access Journals (Sweden)

    Barbara J Meyer

    Full Text Available There is emerging evidence that the supplementation of omega-3 contributes to a decrease in aggressive behaviour in prison populations. A challenge of such research is achieving statistical power against effect sizes which may be affected by the baseline omega-3 index. There are no published data on the blood omega-3 index with studies of this kind to assess the variability of the blood omega-3 index in conjunction with aggression and attention deficit assessments.To determine if the variance of the omega-3 index is correlated with aggressive and attention deficit behaviour in a prison population.136 adult male prisoners were recruited from South Coast Correctional Centre (SCCC, NSW Australia. A 7 point categorisation was used to quantify levels of aggressive behaviour (4 weeks from individual SCCC case notes, whereby higher scores correspond to increasingly aggressive behaviour. Study participants completed the Aggression Questionnaire (AQ and the Brown's Attention Deficit Disorder Scales (BADDS, provided a blood sample for erythrocyte fatty acid analysis using gas chromatography and the omega-3 index was calculated.The baseline omega-3 index ranged from 2.3% to 10.3%, indicating that some participants already had substantial omega-3 intake, however a median of 4.7% indicated a lower overall omega-3 intake than the general Australian population. Assessment of aggressive and attention deficit behaviour shows that there were negative correlations between baseline omega-3 index and baseline aggression categorisation scores (r = -0.21, P = 0.016; total AQ score (r = -0.234, P = 0.011; Anger (r = -0.222 p = 0.016; Hostility AQ (r = -0.239, P = 0.009; indirect aggression (r = -0.188 p = 0.042; total BADDS (r = -0.263, p = 0.005; Activation (r = -0.224, p = 0.016; Attention (r = -0.192, p = 0.043; Effort (r = -0.253, p = 0.007; Affect (r = -0.330, p = 0.000 and Memory (r = -0.240, p = 0.010.There is a high variability in omega-3 status of a NSW prison

  14. Seasonal variation in stable carbon and nitrogen isotope values of bats reflect environmental baselines.

    Directory of Open Access Journals (Sweden)

    Ana G Popa-Lisseanu

    Full Text Available The stable carbon and nitrogen isotope composition of animal tissues is commonly used to trace wildlife diets and analyze food chains. Changes in an animal's isotopic values over time are generally assumed to indicate diet shifts or, less frequently, physiological changes. Although plant isotopic values are known to correlate with climatic seasonality, only a few studies restricted to aquatic environments have investigated whether temporal isotopic variation in consumers may also reflect environmental baselines through trophic propagation. We modeled the monthly variation in carbon and nitrogen isotope values in whole blood of four insectivorous bat species occupying different foraging niches in southern Spain. We found a common pattern of isotopic variation independent of feeding habits, with an overall change as large as or larger than one trophic step. Physiological changes related to reproduction or to fat deposition prior to hibernation had no effect on isotopic variation, but juvenile bats had higher δ13C and δ15N values than adults. Aridity was the factor that best explained isotopic variation: bat blood became enriched in both 13C and 15N after hotter and/or drier periods. Our study is the first to show that consumers in terrestrial ecosystems reflect seasonal environmental dynamics in their isotope values. We highlight the danger of misinterpreting stable isotope data when not accounting for seasonal isotopic baselines in food web studies. Understanding how environmental seasonality is integrated in animals' isotope values will be crucial for developing reliable methods to use stable isotopes as dietary tracers.

  15. Baseline Antihypertensive Drug Count and Patient Response to Hypertension Medication Management. (United States)

    Crowley, Matthew J; Olsen, Maren K; Woolson, Sandra L; King, Heather A; Oddone, Eugene Z; Bosworth, Hayden B


    Telemedicine-based medication management improves hypertension control, but has been evaluated primarily in patients with low antihypertensive drug counts. Its impact on patients taking three or more antihypertensive agents is not well-established. To address this evidence gap, the authors conducted an exploratory analysis of an 18-month, 591-patient trial of telemedicine-based hypertension medication management. Using general linear models, the effect of medication management on blood pressure for patients taking two or fewer antihypertensive agents at study baseline vs those taking three or more was compared. While patients taking two or fewer antihypertensive agents had a significant reduction in systolic blood pressure with medication management, those taking three or more had no such response. The between-subgroup effect difference was statistically significant at 6 months (-6.4 mm Hg [95% confidence interval, -12.2 to -0.6]) and near significant at 18 months (-6.0 mm Hg [95% confidence interval, -12.2 to 0.2]). These findings suggest that baseline antihypertensive drug count may impact how patients respond to hypertension medication management and emphasize the need to study management strategies specifically in patients taking three or more antihypertensive medications.

  16. eQuilibrator--the biochemical thermodynamics calculator. (United States)

    Flamholz, Avi; Noor, Elad; Bar-Even, Arren; Milo, Ron


    The laws of thermodynamics constrain the action of biochemical systems. However, thermodynamic data on biochemical compounds can be difficult to find and is cumbersome to perform calculations with manually. Even simple thermodynamic questions like 'how much Gibbs energy is released by ATP hydrolysis at pH 5?' are complicated excessively by the search for accurate data. To address this problem, eQuilibrator couples a comprehensive and accurate database of thermodynamic properties of biochemical compounds and reactions with a simple and powerful online search and calculation interface. The web interface to eQuilibrator ( enables easy calculation of Gibbs energies of compounds and reactions given arbitrary pH, ionic strength and metabolite concentrations. The eQuilibrator code is open-source and all thermodynamic source data are freely downloadable in standard formats. Here we describe the database characteristics and implementation and demonstrate its use.

  17. Precise baseline determination for the TanDEM-X mission (United States)

    Koenig, Rolf; Moon, Yongjin; Neumayer, Hans; Wermuth, Martin; Montenbruck, Oliver; Jäggi, Adrian

    The TanDEM-X mission will strive for generating a global precise Digital Elevation Model (DEM) by way of bi-static SAR in a close formation of the TerraSAR-X satellite, already launched on June 15, 2007, and the TanDEM-X satellite to be launched in May 2010. Both satellites carry the Tracking, Occultation and Ranging (TOR) payload supplied by the GFZ German Research Centre for Geosciences. The TOR consists of a high-precision dual-frequency GPS receiver, called Integrated GPS Occultation Receiver (IGOR), and a Laser retro-reflector (LRR) for precise orbit determination (POD) and atmospheric sounding. The IGOR is of vital importance for the TanDEM-X mission objectives as the millimeter level determination of the baseline or distance between the two spacecrafts is needed to derive meter level accurate DEMs. Within the TanDEM-X ground segment GFZ is responsible for the operational provision of precise baselines. For this GFZ uses two software chains, first its Earth Parameter and Orbit System (EPOS) software and second the BERNESE software, for backup purposes and quality control. In a concerted effort also the German Aerospace Center (DLR) generates precise baselines independently with a dedicated Kalman filter approach realized in its FRNS software. By the example of GRACE the generation of baselines with millimeter accuracy from on-board GPS data can be validated directly by way of comparing them to the intersatellite K-band range measurements. The K-band ranges are accurate down to the micrometer-level and therefore may be considered as truth. Both TanDEM-X baseline providers are able to generate GRACE baselines with sub-millimeter accuracy. By merging the independent baselines by GFZ and DLR, the accuracy can even be increased. The K-band validation however covers solely the along-track component as the K-band data measure just the distance between the two GRACE satellites. In addition they inhibit an un-known bias which must be modelled in the comparison, so the

  18. Blood pressure measurement (United States)

    Diastolic blood pressure; Systolic blood pressure; Blood pressure reading; Measuring blood pressure ... or your health care provider will wrap the blood pressure cuff snugly around your upper arm. The lower ...

  19. Blood Count Tests (United States)

    Your blood contains red blood cells (RBC), white blood cells (WBC), and platelets. Blood count tests measure the number and types of cells in your blood. This helps doctors check on your overall health. ...

  20. Evaluation of biochemical and ultrasonographic measurements as indicators of undernutrition in cattle


    S. Strydom; S. Agenas; Heath, M F; C.J.C. Phillips; G.H. Rautenbach; P.N. Thompson


    Body condition scoring (BCS) gives an indication of the nutritional status of an animal and it is thus an invaluable management tool in domestic livestock systems. It is, however, subjective. This study aimed at identifying biochemical indicators which could be objectively used as an indicator of undernutrition in ruminants. Blood samples were collected from 50 cattle with BCS ≤ 1 and from 50 cattle with BCS ≥ 2.5, using a 0-5 scale, and analysed for albumin, urea, creatinine, fru...

  1. Haematological and biochemical reference intervals for free-ranging brown bears (Ursus arctos) in Sweden

    DEFF Research Database (Denmark)

    Græsli, Anne Randi; Fahlman, Åsa; Evans, Alina L.;


    BackgroundEstablishment of haematological and biochemical reference intervals is important to assess health of animals on individual and population level. Reference intervals for 13 haematological and 34 biochemical variables were established based on 88 apparently healthy free-ranging brown bears...... bears in Sweden.ResultsThe following variables were not affected by host characteristics: red blood cell, white blood cell, monocyte and platelet count, alanine transaminase, amylase, bilirubin, free fatty acids, glucose, calcium, chloride, potassium, and cortisol. Age differences were seen...... for the majority of the haematological variables, whereas sex influenced only mean corpuscular haemoglobin concentration, aspartate aminotransferase, lipase, lactate dehydrogenase, ß-globulin, bile acids, triglycerides and sodium.ConclusionsThe biochemical and haematological reference intervals provided...

  2. Advances in Biochemical Indices of Zooplankton Production. (United States)

    Yebra, L; Kobari, T; Sastri, A R; Gusmão, F; Hernández-León, S


    Several new approaches for measuring zooplankton growth and production rates have been developed since the publication of the ICES (International Council for the Exploration of the Sea) Zooplankton Methodology Manual (Harris et al., 2000). In this review, we summarize the advances in biochemical methods made in recent years. Our approach explores the rationale behind each method, the design of calibration experiments, the advantages and limitations of each method and their suitability as proxies for in situ rates of zooplankton community growth and production. We also provide detailed protocols for the existing methods and information relevant to scientists wanting to apply, calibrate or develop these biochemical indices for zooplankton production.

  3. Deviation of Biochemical Variables in Dairy Cows with Reproductive Disorders - Data

    Directory of Open Access Journals (Sweden)

    Jožica Ježek


    Full Text Available The aim of the study was to evaluate the results of biochemical profiles of cows from farms with reproductive disorders. The results of blood examinations collected from 205 dairy cows, which originated from 72 Slovenian farms, were analysed. In blood samples total serum protein (TSP, albumin (Alb, urea, calcium (Ca, inorganic phosphate (iP, sodium (Na, potassium (K, chlorine (Cl and betacarotene concentration were measured. Data were compared with normal reference values for cattle. The results were evaluated regarding the normal values for cattle. The descriptive statistics and percentage of cows deviating from normal values for investigated variables were calculated. The mean values of investigated variables were inside reference intervals for cattle. The results of biochemical examination show deviation from reference interval in 52.3% of cows for TSP, 70.8% for urea, 39.8% for iP, 27.5% for Na, 36.2% for K, 1.1% for Cl, 37.6% for beta carotene and 6.8% for Ca. The results of biochemical investigations revealed the greatest deviations in concentrations of TSP, urea, Na, K and carotene which can be affected by different factors (nutrition, health status, stage of lactation and season. Taking into account all of these factors may be blood biochemistry a useful tool also in identifying deficiencies in the diet.

  4. Evaluation of physiological and biochemical responses in different seasons in Surti buffaloes

    Directory of Open Access Journals (Sweden)

    Sandhya S. Chaudhary


    Full Text Available Aim: This study was conducted to evaluate the impact of hot dry, hot humid and comfortable season on physiological, hematological, biochemical, and oxidative stress parameters in Surti buffaloes. Materials and Methods: Ten lactating Surti buffaloes of similar physiological status were selected. Based on the temperature-humidity index (THI, their natural exposure to the environment was categorized as hot dry (THI1, hot humid (THI2 and moderate winter/comfort season (THI3. Blood/serum samples were collected and analyzed for physiological, hematological, biochemical, and oxidative stress parameters. The results were analyzed using standard statistical methods. Results: With increase in THI, significant rise in physiological parameters such as respiration rate (RR, hematological parameters such as red blood cell (RBC, hematocrit, hemoglobin (Hb and mean cell Hb concentration (MCHC, biochemical parameters such as alanine aminotransferase (ALT, Na, K, creatinine, blood urea nitrogen, Mn, Cu and Zn, hormones such as cortisol and oxidative stress parameters such as glutathione peroxidase (GPx, superoxide dismutase (SOD, lipid peroxide (LPO and total antioxidant status (TAS and significant decline in glucose, cholesterol and triiodothyronine (T3 was observed. Conclusion: It was concluded that THI is a sensitive indicator of heat stress and is impacted by ambient temperature more than the relative humidity in buffaloes. Higher THI is associated with significantly increased RR, total RBC count, Hb, hematocrit, MCHC, ALT, urea, sodium, creatinine, triiodothyronine, SOD, GPx, LPO and TAS and with significant decrease in glucose, cholesterol and triiodothyronine (T3.

  5. Parameters Obtained by Hepatobiliary Scintigraphy Have Significant Correlation with Biochemical Factors Early After Liver Transplantation

    Energy Technology Data Exchange (ETDEWEB)

    Kansoul, H.A.; Axelsson, R.; Yamamoto, S.; Savicheva, I.; Aspelin, P.; Ericzon, B.G.; Gjertsen, H. [Div. of Transplantation Surgery and Div. of Radiology, Dept. of Clinical Science, Intervention, and Technology, Karolinska Inst., Karolinska Univ. Hospital, Stockholm (Sweden)


    Background: Early postoperative hepatobiliary scintigraphy after liver transplantation is performed worldwide, but data on its significance for graft function are currently limited. Purpose: To examine the correlation between the result of early postoperative hepatobiliary scintigraphy and pre- and postoperative biochemical parameters in liver transplantation (LTx) patients. Material and Methods: Six parameters of hepatobiliary scintigraphy using {sup 99m}Tc mebrofenin were statistically analyzed in 108 LTx patients: 1) half-life of the activity of elimination of mebrofenin from the blood; 2) total clearance of mebrofenin from the blood due to all possible routes; 3) half-life of the activity due to liver uptake; 4) clearance of mebrofenin from the blood due to liver uptake; 5) time to maximal uptake in the liver; and 6) the hepatic extraction fraction (HEF) and biochemical data. Analysis between patients with preoperative normal liver function, familial amyloid polyneuropathy (FAP), and end-stage liver disease (non-FAP) was also performed. Results: Univariate and multivariate analysis revealed that total bilirubin postoperative day 3 correlated with all three scintigraphic parameters, and peak aspartate aminotransferase and alanine aminotransferase correlated with HEF. The analysis between patients with FAP and non-FAP revealed no significant difference of scintigraphic data between the two groups. Conclusion: A significant correlation between early postoperative scintigraphic results and biochemical parameters was demonstrated.

  6. Hematological parameters in relation to age, sex and biochemical values for mute swans (Cygnus olor). (United States)

    Dolka, B; Włodarczyk, R; Zbikowski, A; Dolka, I; Szeleszczuk, P; Kluciński, W


    The knowledge of the correct morphological and biochemical parameters in mute swans is an important indicator of their health status, body condition, adaptation to habitat and useful diagnostic tools in veterinary practice and ecological research. The aim of the study was to obtain hematological parameters in relation to age, sex and serum biochemistry values in wild-living mute swans. We found the significant differences in the erythrocyte count, hematocrit, hemoglobin concentration and erythrocyte sedimentation rate in relation to age of mute swans. There were no differences in hematological values between males and females. The leukogram and H/L ratio did not vary by age and sex in swans. Among of biochemical parameters the slightly increased AST, ALP, CK, K, urea, decreased CHOL and TG values were recorded. As far as we know, this is the first study in which the morphometric parameters of blood cells in mute swans were presented. We found extremely low concentration of lead in blood (at subthreshold level). No blood parasites were found in blood smears. The analysis of body mass and biometric parameters revealed a significant differences dependent on age and sex. No differences in the scaled mass index were found. Our results represent a normal hematologic and blood chemistry values and age-sex related changes, as reference values for the mute swan.

  7. Rhythmic 24-hour variations of frequently used clinical biochemical parameters in healthy young males - The Bispebjerg study of diurnal variations

    DEFF Research Database (Denmark)

    Sennels, Henriette P; Jørgensen, Henrik L; Gøtze, Jens Peter;


    Purpose. To evaluate the influence of time of day on the circulating concentrations of 14 frequently used clinical biochemical parameters in the Bispebjerg study of diurnal variations. Materials and methods. Venous blood samples were obtained under controlled environmental, activities and food...

  8. Effects of L-alanyl-glutamine upon the blood and kidney biochemical parameters in the rat hind limb model of ischemia/reperfusion Efeitos da L-alanil-glutamina sobre os parâmetros bioquímicos do sangue e do rim no rato submetido à isquemia/reperfusão do membro pélvico



    PURPOSE: To investigate the effects of l-alanyl-glutamine (Ala-Gln) intragastric administration upon blood and kidney metabolic parameters alterations in rats subjected to ischemia/reperfusion of hind limb. METHODS: Forty-eight male rats were randomized in 2 groups offered via gavage either saline 2.0 mL (G-1) or Ala-Gln solution 0.75 mgKg-1(G-2) once a day at 7 AM during 7 days. One-hour after the last gavage (Day 7) all rats were submitted to ether anesthesia, laparotomy and clamping of the...

  9. Characterizing multistationarity regimes in biochemical reaction networks.

    Directory of Open Access Journals (Sweden)

    Irene Otero-Muras

    Full Text Available Switch like responses appear as common strategies in the regulation of cellular systems. Here we present a method to characterize bistable regimes in biochemical reaction networks that can be of use to both direct and reverse engineering of biological switches. In the design of a synthetic biological switch, it is important to study the capability for bistability of the underlying biochemical network structure. Chemical Reaction Network Theory (CRNT may help at this level to decide whether a given network has the capacity for multiple positive equilibria, based on their structural properties. However, in order to build a working switch, we also need to ensure that the bistability property is robust, by studying the conditions leading to the existence of two different steady states. In the reverse engineering of biological switches, knowledge collected about the bistable regimes of the underlying potential model structures can contribute at the model identification stage to a drastic reduction of the feasible region in the parameter space of search. In this work, we make use and extend previous results of the CRNT, aiming not only to discriminate whether a biochemical reaction network can exhibit multiple steady states, but also to determine the regions within the whole space of parameters capable of producing multistationarity. To that purpose we present and justify a condition on the parameters of biochemical networks for the appearance of multistationarity, and propose an efficient and reliable computational method to check its satisfaction through the parameter space.

  10. Biochemical Applications in the Analytical Chemistry Lab (United States)

    Strong, Cynthia; Ruttencutter, Jeffrey


    An HPLC and a UV-visible spectrophotometer are identified as instruments that helps to incorporate more biologically-relevant experiments into the course, in order to increase the students understanding of selected biochemistry topics and enhances their ability to apply an analytical approach to biochemical problems. The experiment teaches…

  11. 2009 Biochemical Conversion Platform Review Report

    Energy Technology Data Exchange (ETDEWEB)

    Ferrell, John [Office of Energy Efficiency and Renewable Energy (EERE), Washington, DC (United States)


    This document summarizes the recommendations and evaluations provided by an independent external panel of experts at the U.S. Department of Energy Biomass Program’s Biochemical Conversion platform review meeting, held on April 14-16, 2009, at the Sheraton Denver Downtown, Denver, Colorado.

  12. Biochemical and Anatomical Characteristics of Dolphin Muscles. (United States)


    hexosamines. These data suggest a biochemical difference in dolphin and rat tendon, which may be of relevance to the unique myofascial design of the...glycosaminoglycan (GAG) chains . The GAG chain is a polymer of repeating disaccharides, each disaccharide containing a hexosamine and either a

  13. Biochemical Thermodynamics under near Physiological Conditions (United States)

    Mendez, Eduardo


    The recommendations for nomenclature and tables in Biochemical Thermodynamics approved by IUBMB and IUPAC in 1994 can be easily introduced after the chemical thermodynamic formalism. Substitution of the usual standard thermodynamic properties by the transformed ones in the thermodynamic equations, and the use of appropriate thermodynamic tables…

  14. Atmospheric pressure loading parameters from very long baseline interferometry observations (United States)

    Macmillan, D. S.; Gipson, John M.


    Atmospheric mass loading produces a primarily vertical displacement of the Earth's crust. This displacement is correlated with surface pressure and is large enough to be detected by very long baseline interferometry (VLBI) measurements. Using the measured surface pressure at VLBI stations, we have estimated the atmospheric loading term for each station location directly from VLBI data acquired from 1979 to 1992. Our estimates of the vertical sensitivity to change in pressure range from 0 to -0.6 mm/mbar depending on the station. These estimates agree with inverted barometer model calculations (Manabe et al., 1991; vanDam and Herring, 1994) of the vertical displacement sensitivity computed by convolving actual pressure distributions with loading Green's functions. The pressure sensitivity tends to be smaller for stations near the coast, which is consistent with the inverted barometer hypothesis. Applying this estimated pressure loading correction in standard VLBI geodetic analysis improves the repeatability of estimated lengths of 25 out of 37 baselines that were measured at least 50 times. In a root-sum-square (rss) sense, the improvement generally increases with baseline length at a rate of about 0.3 to 0.6 ppb depending on whether the baseline stations are close to the coast. For the 5998-km baseline from Westford, Massachusetts, to Wettzell, Germany, the rss improvement is about 3.6 mm out of 11.0 mm. The average rss reduction of the vertical scatter for inland stations ranges from 2.7 to 5.4 mm.

  15. Detection of abrupt baseline length changes using cumulative sums (United States)

    Janssen, Volker


    Dynamic processes are usually monitored by collecting a time series of observations, which is then analysed in order to detect any motion or non-standard behaviour. Geodetic examples include the monitoring of dams, bridges, high-rise buildings, landslides, volcanoes and tectonic motion. The cumulative sum (CUSUM) test is recognised as a popular means to detect changes in the mean and/or the standard deviation of a time series and has been applied to various monitoring tasks. This paper briefly describes the CUSUM technique and how it can be utilised for the detection of small baseline length changes by differencing two perpendicular baselines sharing a common site. A simulation is carried out in order to investigate the expected behaviour of the resulting CUSUM charts for a variety of typical deformation monitoring scenarios. This simulation shows that using first differences (between successive epochs) as input, rather than the original baseline lengths, produces clear peaks or jumps in the differenced CUSUM time series when a sudden change in baseline length occurs. These findings are validated by analysing several GPS baseline pairs of a network deployed to monitor the propagation of an active ice shelf rift on the Amery Ice Shelf, East Antarctica.

  16. Combined GPS + BDS for short to long baseline RTK positioning (United States)

    Odolinski, R.; Teunissen, P. J. G.; Odijk, D.


    The BeiDou Navigation Satellite System (BDS) has become fully operational in the Asia-Pacific region and it is of importance to evaluate what BDS brings when combined with the Global Positioning System (GPS). In this contribution we will look at the short, medium and long single-baseline real-time kinematic (RTK) positioning performance. Short baseline refers to when the distance between the two receivers is at most a few kilometers so that the relative slant ionospheric and tropospheric delays can be assumed absent, whereas with medium baseline we refer to when the uncertainty of these ionospheric delays can reliably be modeled as a function of the baseline length. With long baseline we refer to the necessity to parameterize the ionospheric delays and (wet) Zenith Tropospheric Delay (ZTD) as completely unknown. The GNSS real data are collected in Perth, Australia. It will be shown that combining the two systems allows for the use of higher than customary elevation cut-off angles. This can be of particular benefit in environments with restricted satellite visibility such as in open pit mines or urban canyons.

  17. LBCS: the LOFAR Long-Baseline Calibrator Survey

    CERN Document Server

    Jackson, N; Deller, A; Moldón, J; Varenius, E; Morabito, L; Wucknitz, O; Carozzi, T; Conway, J; Drabent, A; Kapinska, A; Orrù, E; Brentjens, M; Blaauw, R; Kuper, G; Sluman, J; Schaap, J; Vermaas, N; Iacobelli, M; Cerrigone, L; Shulevski, A; ter Veen, S; Fallows, R; Pizzo, R; Sipior, M; Anderson, J; Avruch, M; Bell, M; van Bemmel, I; Bentum, M; Best, P; Bonafede, A; Breitling, F; Broderick, J; Brouw, W; Brüggen, M; Ciardi, B; Corstanje, A; de Gasperin, F; de Geus, E; Eislöffel, J; Engels, D; Falcke, H; Garrett, M; Griessmeier, J; Gunst, A; van Haarlem, M; Heald, G; Hoeft, M; Hörandel, J; Horneffer, A; Intema, H; Juette, E; Kuniyoshi, M; van Leeuwen, J; Loose, G; Maat, P; McFadden, R; McKay-Bukowski, D; McKean, J; Mulcahy, D; Munk, H; Pandey-Pommier, M; Polatidis, A; Reich, W; Röttgering, H; Rowlinson, A; Scaife, A; Schwarz, D; Steinmetz, M; Swinbank, J; Thoudam, S; Toribio, M; Vermeulen, R; Vocks, C; van Weeren, R; Wise, M; Yatawatta, S; Zarka, P


    (abridged). We outline LBCS (the LOFAR Long-Baseline Calibrator Survey), whose aim is to identify sources suitable for calibrating the highest-resolution observations made with the International LOFAR Telescope, which include baselines >1000 km. Suitable sources must contain significant correlated flux density (50-100mJy) at frequencies around 110--190~MHz on scales of a few hundred mas. At least for the 200--300-km international baselines, we find around 1 suitable calibrator source per square degree over a large part of the northern sky, in agreement with previous work. This should allow a randomly selected target to be successfully phase calibrated on the international baselines in over 50% of cases. Products of the survey include calibrator source lists and fringe-rate and delay maps of wide areas -- typically a few degrees -- around each source. The density of sources with significant correlated flux declines noticeably with baseline length over the range 200--600~km, with good calibrators on the longest...

  18. Toll Like Receptor 4 Affects the Cerebral Biochemical Changes Induced by MPTP Treatment. (United States)

    Conte, Carmela; Roscini, Luca; Sardella, Roccaldo; Mariucci, Giuseppina; Scorzoni, Stefania; Beccari, Tommaso; Corte, Laura


    The etiology and pathogenesis of Parkinson's disease (PD) are still unclear. However, multiple lines of evidence suggest a critical role of the toll like receptor 4 (TLR4) in inflammatory response and neuronal death. Neuroinflammation may be associated with the misfolding and aggregation of proteins accompanied by a change in their secondary structure. Recent findings also suggest that biochemical perturbations in cerebral lipid content could contribute to the pathogenesis of central nervous system (CNS) disorders, including PD. Thus, it is of great importance to determine the biochemical changes that occur in PD. In this respect, Fourier Transform Infrared (FTIR) spectroscopy represents a useful tool to detect molecular alterations in biological systems in response to stress stimuli. By relying upon FTIR approach, this study was designed to elucidate the potential role of TLR4 in biochemical changes induced by methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) neurotoxin in a mouse model of PD. The analysis of the FTIR spectra was performed in different brain regions of both wild type (WT) and toll like receptor 4-deficient (TLR4(-/-)) mice. It revealed that each brain region exhibited a characteristic molecular fingerprint at baseline, with no significant differences between genotypes. Conversely, WT and TLR4(-/-) mice showed differential biochemical response to MPTP toxicity, principally related to lipid and protein composition. These differences appeared to be characteristic for each brain area. Furthermore, the present study showed that WT mice resulted more vulnerable than TLR4(-/-) animals to striatal dopamine (DA) depletion following MPTP treatment. These results support the hypothesis of a possible involvement of TLR4 in biochemical changes occurring in neurodegeneration.

  19. Functional Testing Protocols for Commercial Building Efficiency Baseline Modeling Software

    Energy Technology Data Exchange (ETDEWEB)

    Jump, David; Price, Phillip N.; Granderson, Jessica; Sohn, Michael


    This document describes procedures for testing and validating proprietary baseline energy modeling software accuracy in predicting energy use over the period of interest, such as a month or a year. The procedures are designed according to the methodology used for public domain baselining software in another LBNL report that was (like the present report) prepared for Pacific Gas and Electric Company: ?Commercial Building Energy Baseline Modeling Software: Performance Metrics and Method Testing with Open Source Models and Implications for Proprietary Software Testing Protocols? (referred to here as the ?Model Analysis Report?). The test procedure focuses on the quality of the software?s predictions rather than on the specific algorithms used to predict energy use. In this way the software vendor is not required to divulge or share proprietary information about how their software works, while enabling stakeholders to assess its performance.

  20. A publication database for optical long baseline interferometry

    CERN Document Server

    Malbet, Fabien; Lawson, Peter; Taillifet, Esther; Lafrasse, Sylvain


    Optical long baseline interferometry is a technique that has generated almost 850 refereed papers to date. The targets span a large variety of objects from planetary systems to extragalactic studies and all branches of stellar physics. We have created a database hosted by the JMMC and connected to the Optical Long Baseline Interferometry Newsletter (OLBIN) web site using MySQL and a collection of XML or PHP scripts in order to store and classify these publications. Each entry is defined by its ADS bibcode, includes basic ADS informations and metadata. The metadata are specified by tags sorted in categories: interferometric facilities, instrumentation, wavelength of operation, spectral resolution, type of measurement, target type, and paper category, for example. The whole OLBIN publication list has been processed and we present how the database is organized and can be accessed. We use this tool to generate statistical plots of interest for the community in optical long baseline interferometry.

  1. Low Blood Glucose (Hypoglycemia) (United States)

    ... Disease, & Other Dental Problems Diabetes & Sexual & Urologic Problems Low Blood Glucose (Hypoglycemia) What is hypoglycemia? Hypoglycemia, also called low blood glucose or low blood sugar, occurs when ...

  2. Environmental baselines: preparing for shale gas in the UK (United States)

    Bloomfield, John; Manamsa, Katya; Bell, Rachel; Darling, George; Dochartaigh, Brighid O.; Stuart, Marianne; Ward, Rob


    Groundwater is a vital source of freshwater in the UK. It provides almost 30% of public water supply on average, but locally, for example in south-east England, it is constitutes nearly 90% of public supply. In addition to public supply, groundwater has a number of other uses including agriculture, industry, and food and drink production. It is also vital for maintaining river flows especially during dry periods and so is essential for maintaining ecosystem health. Recently, there have been concerns expressed about the potential impacts of shale gas development on groundwater. The UK has abundant shales and clays which are currently the focus of considerable interest and there is active research into their characterisation, resource evaluation and exploitation risks. The British Geological Survey (BGS) is undertaking research to provide information to address some of the environmental concerns related to the potential impacts of shale gas development on groundwater resources and quality. The aim of much of this initial work is to establish environmental baselines, such as a baseline survey of methane occurrence in groundwater (National methane baseline study) and the spatial relationships between potential sources and groundwater receptors (iHydrogeology project), prior to any shale gas exploration and development. The poster describes these two baseline studies and presents preliminary findings. BGS are currently undertaking a national survey of baseline methane concentrations in groundwater across the UK. This work will enable any potential future changes in methane in groundwater associated with shale gas development to be assessed. Measurements of methane in potable water from the Cretaceous, Jurassic and Triassic carbonate and sandstone aquifers are variable and reveal methane concentrations of up to 500 micrograms per litre, but the mean value is relatively low at 2km. The geological modelling process will be presented and discussed along with maps combining

  3. Subtracting Technique of Baselines for Capillary Electrophoresis Signals

    Institute of Scientific and Technical Information of China (English)

    WANG Ying; MO Jin-yuan; CHEN Zuan-guang; GAO Yan


    The drifting baselines of capillary electrophoresis affect the veracity of analysis greatly. This paper presents Threshold Fitting Technique(TFT) so as to subtract the baselines from the original signals and emendate the signals. In TFT, wav elet and curve fitting technique are applied synthetically, thresholds are decided by the computer automatically. Many experiments of signal processing indicate that TFT is simple for being used, there are few man-induced factors, and the results are satisfactory. TFT can be applied for noisy signals without any pre-processing.


    Institute of Scientific and Technical Information of China (English)

    Gong Min; Zhang Chuanwu; Huang Shunji


    The change of the equivalent baseline and interferometric phase of cluster SAR satellites is analyzed when the constellation circles around the Earth and the satellites rotate around the center at the same time. The letter provides assessment of baseline error and phase error which influence the precision of height measurement in the across-track interferometric mode. The mathematical model of cluster satellite movement is built, simulation analyses and the curve of height error are presented. The simulation results show that height measurement error can be compensated by the formulae derived in this letter, therefore, the Digital Elevation Models (DEM's) are recovered accurately.

  5. Intermediate baseline appearance experiments and three-neutrino mixing schemes

    CERN Document Server

    Cardall, C Y; Cline, D; Cardall, Christian Y.; Fuller, George M.; Cline, David


    Three-neutrino mixing schemes suggested by Cardall \\& Fuller and Acker \\& Pakvasa are compared and contrasted. Both of these schemes seek to solve the solar and atmospheric neutrino problems {\\em and} to account for the possible neutrino oscillation signal in the LSND experiment. These neutrino oscillation schemes have different atmospheric and solar neutrino signatures that will be discriminated by Super-Kamiokande and SNO. They will also have different signatures in proposed long-baseline accelerator and reactor experiments. In particular, both of these schemes would give dramatic (and dramatically different) signals in an ``intermediate baseline'' experiment, such as the proposed ICARUS detector in the Jura mountains 17 km from CERN.

  6. Hepatitis C treatment response kinetics and impact of baseline predictors

    DEFF Research Database (Denmark)

    Lindh, M; Arnholm, B; Eilard, A


    Summary. The optimal duration of treatment for hepatitis C virus (HCV) infections is highly variable but critical for achieving cure (sustained virological response, SVR). We prospectively investigated the impact of age, fibrosis, baseline viraemia and genotype on the early viral kinetics...... above 400 000 IU/mL were strongly associated with slower second phase declines of HCV RNA. Genotype 2/3 infections responded more rapidly than genotype 1, reaching week 4 negativity (RVR) in 59%vs 22%. We conclude that baseline response predictors such as age, fibrosis and viral load were well reflected...

  7. Solar central electric power generation - A baseline design (United States)

    Powell, J. C.


    The paper presents the conceptual technical baseline design of a solar electric power plant using the central receiver concept, and derives credible cost estimates from the baseline design. The major components of the plant - heliostats, tower, receiver, tower piping, and thermal storage - are discussed in terms of technical and cost information. The assumed peak plant output is 215 MW(e), over 4000 daylight hours. The contribution of total capital investment to energy cost is estimated to be about 55 mills per kwh in mid-1974 dollars.

  8. Detecting dark energy in long baseline neutrino oscillations

    Institute of Scientific and Technical Information of China (English)

    GU Pei-Hong; BI Xiao-Jun; FENG Bo; YOUNG Bing-Lin; ZHANG Xin-Min


    In this paper, we discuss a possibility of studying properties of dark energy in long baseline neutrino oscillation experiments. We consider two types of models of neutrino dark energy. For one type of models the scalar field is taken to be quintessence-like and for the other phantom-like. In these models the scalar fields couple to the neutrinos to give rise to spatially varying neutrino masses. We will show that the two types of models predict different behaviors of the spatial variation of the neutrino masses inside the Earth and consequently result in different signals in long baseline neutrino oscillation experiments.

  9. Future long-baseline neutrino oscillations: View from Asia

    Energy Technology Data Exchange (ETDEWEB)

    Hayato, Yoshinari [Kamioka Observatory, ICRR, The University of Tokyo (Japan)


    Accelerator based long-baseline neutrino oscillation experiments have been playing important roles in revealing the nature of neutrinos. However, it turned out that the current experiments are not sufficient to study two major remaining problems, the CP violation in the lepton sector and the mass hierarchy of neutrinos. Therefore, several new experiments have been proposed. Among of them, two accelerator based long-baseline neutrino oscillation experiments, the J-PARC neutrino beam and Hyper-Kamiokande, and MOMENT, have been proposed in Asia. These two projects are reviewed in this article.

  10. Electret-thermal analysis of blood. (United States)

    Pinchuk, L S; Goldade, V A; Sessler, G M; Kravtsov, A G; Zotov, S V; Tsvetkova, E A


    The thermally stimulated discharge (TSD) method, intended for the analysis of charged dielectrics, was used for human blood research. Above-room-temperature TSD spectra of blood consist of three peaks. There are indications that the low-temperature peak (40-50 degrees C) corresponds to the thermally stimulated destruction of hydrate shells surrounding blood components while the mid-temperature peak (70-90 degrees C) is related to thermal denaturation of blood proteins. The intensive high-temperature peak (105-120 degrees C) is observed when a phase transition of blood, accompanied by the formation of a firm dry film of blood, occurs. The position of the high-temperature peak depends on the blood group. Data is discussed which suggests that the spontaneous "quasi-electret effect" of blood relates to the character of the biochemical processes taking place in human organisms. The TSD method might be used as simple and informative means of diagnostics in cooperation with medical and physical investigations.

  11. Blood type biochemistry and human disease. (United States)

    Ewald, D Rose; Sumner, Susan C J


    Associations between blood type and disease have been studied since the early 1900s when researchers determined that antibodies and antigens are inherited. In the 1950s, the chemical identification of the carbohydrate structure of surface antigens led to the understanding of biosynthetic pathways. The blood type is defined by oligosaccharide structures, which are specific to the antigens, thus, blood group antigens are secondary gene products, while the primary gene products are various glycosyltransferase enzymes that attach the sugar molecules to the oligosaccharide chain. Blood group antigens are found on red blood cells, platelets, leukocytes, plasma proteins, certain tissues, and various cell surface enzymes, and also exist in soluble form in body secretions such as breast milk, seminal fluid, saliva, sweat, gastric secretions, urine, and amniotic fluid. Recent advances in technology, biochemistry, and genetics have clarified the functional classifications of human blood group antigens, the structure of the A, B, H, and Lewis determinants and the enzymes that produce them, and the association of blood group antigens with disease risks. Further research to identify differences in the biochemical composition of blood group antigens, and the relationship to risks for disease, can be important for the identification of targets for the development of nutritional intervention strategies, or the identification of druggable targets. WIREs Syst Biol Med 2016, 8:517-535. doi: 10.1002/wsbm.1355 For further resources related to this article, please visit the WIREs website.

  12. Network of vascular diseases, death and biochemical characteristics in a set of 4,197 patients with type 1 diabetes (The FinnDiane Study

    Directory of Open Access Journals (Sweden)

    Wadén Johan


    Full Text Available Background Cardiovascular disease is the main cause of premature death in patients with type 1 diabetes. Patients with diabetic kidney disease have an increased risk of heart attack or stroke. Accurate knowledge of the complex inter-dependencies between the risk factors is critical for pinpointing the best targets for research and treatment. Therefore, the aim of this study was to describe the association patterns between clinical and biochemical features of diabetic complications. Methods Medical records and serum and urine samples of 4,197 patients with type 1 diabetes were collected from health care centers in Finland. At baseline, the mean diabetes duration was 22 years, 52% were male, 23% had kidney disease (urine albumin excretion over 300 mg/24 h or end-stage renal disease and 8% had a history of macrovascular events. All-cause mortality was evaluated after an average of 6.5 years of follow-up (25,714 patient years. The dataset comprised 28 clinical and 25 biochemical variables that were regarded as the nodes of a network to assess their mutual relationships. Results The networks contained cliques that were densely inter-connected (r > 0.6, including cliques for high-density lipoprotein (HDL markers, for triglycerides and cholesterol, for urinary excretion and for indices of body mass. The links between the cliques showed biologically relevant interactions: an inverse relationship between HDL cholesterol and the triglyceride clique (r P -16, a connection between triglycerides and body mass via C-reactive protein (r > 0.3, P -16 and intermediate-density cholesterol as the connector between lipoprotein metabolism and albuminuria (r > 0.3, P -16. Aging and macrovascular disease were linked to death via working ability and retinopathy. Diabetic kidney disease, serum creatinine and potassium, retinopathy and blood pressure were inter-connected. Blood pressure correlations indicated accelerated vascular aging in individuals with kidney disease

  13. Baseline Tc-99m DTPA renal scintigraphy as a predictor of outcome in children with urinary tract infection

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    Yun, J. G.; An, Y. S.; Lee, M. H.; Cho, C. W.; Yun, S. N.; Pai, G. S [Ajou University Medical Center, Suwon (Korea, Republic of)


    Tc-99m DTPA renal scintigraphy is useful in detecting urinary tract obstruction in patients with urinary tract infection (UTI). We evaluated the prognostic significance of baseline Tc-99m DTPA renal scintigraphy in children with UTI. Among children, who underwent both baseline/follow-up Tc-99m DMSA scintigraphies and baseline Tc-99m DTP A scintigraphy for evaluation of UTI, 32 patients with unilateral cortical defects on baseline Tc-99m DMSA scintigraphy were included in the study. The outcome of cortical defects was evaluated on follow-up Tc-99m DMSA scintigraphy by visual analysis. ROIs were drawn on the Tc-99m DPTA scintigraphy for calculation of ipsilateral to contralateral kidney ratio (ICR) at blood flow phase (< 60s, BFP) and cortical uptake phase (1-5 min, CUP). Median follow-up period of Tc-99m DMSA scintigraphy was 3.2 months (1.4 - 14 months). There were 24 patients with healing cortical defects and 8 with cortical scarring. Average ICRs of patients with healing defects were 1.11 {+-} 0.18 (0.44 - 1.57) at BFP and 0.97 {+-} 0.21 (0.31 - 1.28) at CUP, while those of patients with cortical scarring were 0.97 {+-} 0.47 (0.21 - 0.89) at BFP and 0.75 {+-} 0.49 (0.19 - 1.65) at CUP. ICR more than 0.9 was determined as a good prognostic indicator. The sensitivity, specificity, positive predictive value, and negative predictive value of ICR was 83.3%, 100%, 100% and 66.7% at BFP, and 79.0%, 62.5%, 86.4% and 50.0% at CUP. Ipsilateral to contralateral kidney ratio on baseline Tc-99m DPTA renal scintigraphy, especially at blood flow phase, is helpful in predicting outcome of children with UTI.

  14. Baseline reef health surveys at Bangka Island (North Sulawesi, Indonesia reveal new threats

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    Massimo Ponti


    Full Text Available Worldwide coral reef decline appears to be accompanied by an increase in the spread of hard coral diseases. However, whether this is the result of increased direct and indirect human disturbances and/or an increase in natural stresses remains poorly understood. The provision of baseline surveys for monitoring coral health status lays the foundations to assess the effects of any such anthropogenic and/or natural effects on reefs. Therefore, the objectives of this present study were to provide a coral health baseline in a poorly studied area, and to investigate possible correlations between coral health and the level of anthropogenic and natural disturbances. During the survey period, we recorded 20 different types of coral diseases and other compromised health statuses. The most abundant were cases of coral bleaching, followed by skeletal deformations caused by pyrgomatid barnacles, damage caused by fish bites, general pigmentation response and galls caused by cryptochirid crabs. Instances of colonies affected by skeletal eroding bands, and sedimentation damage increased in correlation to the level of bio-chemical disturbance and/or proximity to villages. Moreover, galls caused by cryptochirid crabs appeared more abundant at sites affected by blast fishing and close to a newly opened metal mine. Interestingly, in the investigated area the percentage of corals showing signs of ‘common’ diseases such as black band disease, brown band disease, white syndrome and skeletal eroding band disease were relatively low. Nevertheless, the relatively high occurrence of less common signs of compromised coral-related reef health, including the aggressive overgrowth by sponges, deserves further investigation. Although diseases appear relatively low at the current time, this area may be at the tipping point and an increase in activities such as mining may irredeemably compromise reef health.

  15. Kombucha tea fermentation: Microbial and biochemical dynamics. (United States)

    Chakravorty, Somnath; Bhattacharya, Semantee; Chatzinotas, Antonis; Chakraborty, Writachit; Bhattacharya, Debanjana; Gachhui, Ratan


    Kombucha tea, a non-alcoholic beverage, is acquiring significant interest due to its claimed beneficial properties. The microbial community of Kombucha tea consists of bacteria and yeast which thrive in two mutually non-exclusive compartments: the soup or the beverage and the biofilm floating on it. The microbial community and the biochemical properties of the beverage have so far mostly been described in separate studies. This, however, may prevent understanding the causal links between the microbial communities and the beneficial properties of Kombucha tea. Moreover, an extensive study into the microbial and biochemical dynamics has also been missing. In this study, we thus explored the structure and dynamics of the microbial community along with the biochemical properties of Kombucha tea at different time points up to 21 days of fermentation. We hypothesized that several biochemical properties will change during the course of fermentation along with the shifts in the yeast and bacterial communities. The yeast community of the biofilm did not show much variation over time and was dominated by Candida sp. (73.5-83%). The soup however, showed a significant shift in dominance from Candida sp. to Lachancea sp. on the 7th day of fermentation. This is the first report showing Candida as the most dominating yeast genus during Kombucha fermentation. Komagateibacter was identified as the single largest bacterial genus present in both the biofilm and the soup (~50%). The bacterial diversity was higher in the soup than in the biofilm with a peak on the seventh day of fermentation. The biochemical properties changed with the progression of the fermentation, i.e., beneficial properties of the beverage such as the radical scavenging ability increased significantly with a maximum increase at day 7. We further observed a significantly higher D-saccharic acid-1,4-lactone content and caffeine degradation property compared to previously described Kombucha tea fermentations. Our

  16. Baseline oxygenation in the brain: Correlation between respiratory-calibration and susceptibility methods. (United States)

    Fan, Audrey P; Schäfer, Andreas; Huber, Laurentius; Lampe, Leonie; von Smuda, Steffen; Möller, Harald E; Villringer, Arno; Gauthier, Claudine J


    New MRI methods for noninvasive imaging of baseline oxygen extraction fraction (OEF) in the brain show great promise. Quantitative O2 imaging (QUO2) applies a biophysical model to measure OEF in tissue from BOLD, cerebral blood flow (CBF), and end-tidal O2 (ETO2) signals acquired during two or more gas manipulations. Alternatively, quantitative susceptibility mapping (QSM) maps baseline OEF along cerebral vessels based on the deoxyhemoblogin (dHb) susceptibility shift between veins and water. However, these approaches have not been carefully compared to each other or to known physiological signals. The aims of this study were to compare OEF values by QUO2 and QSM; and to see if baseline OEF relates to BOLD and CBF changes during a visual task. Simultaneous BOLD and arterial spin labeling (ASL) scans were acquired at 7T in 11 healthy subjects continuously during hypercapnia (5% CO2, 21% O2), hyperoxia (100% O2), and carbogen (5% CO2, 95% O2) for QUO2 analysis. Separate BOLD-ASL scans were acquired during a checkerboard stimulus to identify functional changes in the visual cortex. Gradient echo phase images were also collected at rest for QSM reconstruction of OEF along cerebral veins draining the visual cortex. Mean baseline OEF was (43.5±14)% for QUO2 with two gases, (42.3±17)% for QUO2 with three gases, and (29.4±3)% for QSM across volunteers. Three-gas QUO2 values of OEF correlated with QSM values of OEF (P=0.03). However, Bland-Altman analysis revealed that QUO2 tended to measure higher baseline OEF with respect to QSM, which likely results from underestimation of the hyperoxic BOLD signal and low signal-to-noise ratio of the ASL acquisitions. Across subjects, the percent CBF change during the visual task correlated with OEF measured by 3-gas QUO2 (P<0.04); and by QSM (P=0.035), providing evidence that the new methods measure true variations in brain physiology across subjects.

  17. Haematological and serum biochemical responses of chickens to hydric stress. (United States)

    Chikumba, N; Swatson, H; Chimonyo, M


    Dehydration can be extremely damaging to the performance and welfare of indigenous chickens. The effect of water restriction on haematological and biochemical parameters was compared in Naked Neck (NNK) and Ovambo (OVB) chickens. A total of 54 8-week-old pullets each of NNK and OVB chickens with an initial average weight of 641 ± 10 g/bird were randomly assigned to three water intake treatments with three replications, each having six birds. The water restriction treatments were ad libitum, 70% and 40% of ad libitum intake. Nine experimental pens with a floor space of 3.3 m2 per strain were used. Feed was provided ad libitum. Packed cell volume (PCV), erythrocyte count (RBC), mean corpuscular volume (MCV) and total leucocyte count (WBC), and biochemical parameters (uric acid (UA)), creatinine (CREAT), total protein (TP), albumin (ALB), globulin (GLOB), triglyceride (TGA), total cholesterol (TC), high- (HDLC) and low- (LDLC) density lipoprotein cholesterol and activity of alanine transaminase (ALT), alkaline phosphatase (ALP) and aspartate transaminase (AST) were determined from blood collected after 60 days of water restriction. PCV was higher (P chickens offered water ad libitum, but similar in birds offered 70% and 40% of ad libitum. There were no differences in RBC and MCV values between strains, but MCV was higher in birds on 40% than 70% of ad libitum water intake, irrespective of strain. Naked neck chickens had higher (P chickens. The opposite was observed for UA. ALT activity indicated that liver function was not affected by water restriction. It was concluded that the two strains can withstand up to 40% of ad libitum water restriction, but NNK tolerated water stress better than OVB chickens.

  18. Dietary intakes and some biochemical markers in hemodialysis patients

    Directory of Open Access Journals (Sweden)

    Emine Yassibas


    Full Text Available Purpose: Intake of energy and some nutrients is frequently inadequate in dialysis patients because of eating and appetite disorders due to uremic syndrome. Inadequate energy and protein intake cause to malnutrition. Anthropometric measurements, biochemical markers and dietary intake records have great importance in determining the nutritional status of hemodialysis (HD patients. Therefore this study was planned to determination of daily energy, nutrient intake and some biochemical markers of adult hemodialysis patients. Method and Material:  A hundred and thirty six (male = 73, female = 63 clinically stable HD patients were enrolled in this study. All patients were taken 24-hour dietary recall. The daily energy and nutrient intakes of patients were calculated by food consumption records and anthropometric measurements were taken. Results: The mean age was found 45.5±13.51 years. The ratio of patients with body mass index (BMI below 18.5 kg/m2 was 7.4% and 25 kg/m2 or upper was 16.9%. Blood hemoglobin, hematocrit, albumin and total protein levels were below the normal levels. The daily dietary energy and protein intakes were found under the recommended level in the study period. A significant positive correlation was found between daily protein intake and serum albumin levels (r=0.210, p=0.014, daily protein intake and serum total protein levels (r=0.201, p=0.019, daily energy intake and serum total protein levels (r=0.178, p=0.039. Conclusions: HD patients often have low protein and energy intakes and most of them suffer from malnutrition. Therefore nutritional status of patients should be evaluated and periodically nutrition education should be given for improving eating habits and increasing dietary compliance is recommended.

  19. Biochemical Effects of Carbohydrate Supplementation in