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Sample records for blocks endocytic traffic

  1. Chemical Genetic Screening Identifies Sulfonamides That Raise Organellar pH and Interfere with Endocytic and Exocytic Membrane Traffic

    OpenAIRE

    Nieland, Thomas J. F.; Feng, Yan; Brown, Jing Xu; Chuang, Tuan Daniel; Buckett, Peter D.; Wang, Jin; Xie, Xiao-Song; McGraw, Timothy E.; Kirchhausen, Tomas; Wessling-Resnick, Marianne

    2004-01-01

    Chemical genetics seeks to identify small molecules that afford functional dissection of cell biological pathways. Previous screens for small molecule inhibitors of exocytic membrane traffic yielded the identification and characterization of several compounds that block traffic from the Golgi to the cell surface as well as transport from the endoplasmic reticulum to the Golgi network. Here, we screened these inhibitors for potential effects on endocytic membrane traffic. Two structurally rela...

  2. Traffic jam hypothesis: Relationship between endocytic dysfunction and Alzheimer's disease.

    Science.gov (United States)

    Kimura, Nobuyuki; Yanagisawa, Katsuhiko

    2017-07-08

    Membrane trafficking pathways, like the endocytic pathway, carry out fundamental cellular processes that are essential for normal functioning. One such process is regulation of cell surface receptor signaling. A growing body of evidence suggests that β-amyloid protein (Aβ) plays a key role in Alzheimer's disease (AD) pathogenesis. Cleavage of Aβ from its precursor, β-amyloid precursor protein (APP), occurs through the endocytic pathway in neuronal cells. In early-stage AD, intraneuronal accumulation of abnormally enlarged endosomes is common, indicating that endosome trafficking is disrupted. Strikingly, genome-wide association studies reveal that several endocytosis-related genes are associated with AD onset. Also, recent studies demonstrate that alteration in endocytosis induces not only Aβ pathology but also the propagation of tau protein pathology, another key pathological feature of AD. Endocytic dysfunction can disrupt neuronal physiological functions, such as synaptic vesicle transport and neurotransmitter release. Thus, "traffic jams" in the endocytic pathway may be involved in AD pathogenesis and may serve as a novel target for the development of new therapeutics. Copyright © 2017 Elsevier Ltd. All rights reserved.

  3. Chemical Genetic Screening Identifies Sulfonamides That Raise Organellar pH and Interfere with Endocytic and Exocytic Membrane Traffic

    Science.gov (United States)

    Nieland, Thomas J. F.; Feng, Yan; Brown, Jing Xu; Chuang, Tuan Daniel; Buckett, Peter D.; Wang, Jin; Xie, Xiao-Song; McGraw, Timothy E.; Kirchhausen, Tomas; Wessling-Resnick, Marianne

    2008-01-01

    Chemical genetics seeks to identify small molecules that afford functional dissection of cell biological pathways. Previous screens for small molecule inhibitors of exocytic membrane traffic yielded the identification and characterization of several compounds that block traffic from the Golgi to the cell surface as well as transport from the endoplasmic reticulum to the Golgi network. Here, we screened these inhibitors for potential effects on endocytic membrane traffic. Two structurally related sulfonamides were found to be potent and reversible inhibitors of transferrin-mediated iron uptake. These inhibitors do not block endoplasmic reticulum-to-Golgi transport, but do disrupt Golgi-to-cell surface traffic. The compounds are members of a novel class of sulfonamides that elevate endosomal and lysosomal pH, down-regulate cell surface receptors, and impair recycling of internalized transferrin receptors to the plasma membrane. In vitro experiments revealed that the sulfonamides directly inhibit adenosine triphosphate (ATP) hydrolysis by the V-ATPase and that they also possess a potent proton ionophore activity. While maintenance of organellar pH is known to be a critical factor in both endocytosis and exocytosis, the precise role of acidification, beyond the uncoupling of ligands from their receptors, remains largely unknown. Identification of this novel class of sulfonamide inhibitors provides new chemical tools to better understand the function of organelle pH in membrane traffic and the activity of V-ATPases in particular. PMID:15180825

  4. A-RAF kinase functions in ARF6 regulated endocytic membrane traffic.

    Directory of Open Access Journals (Sweden)

    Elena Nekhoroshkova

    Full Text Available BACKGROUND: RAF kinases direct ERK MAPK signaling to distinct subcellular compartments in response to growth factor stimulation. METHODOLOGY/PRINCIPAL FINDINGS: Of the three mammalian isoforms A-RAF is special in that one of its two lipid binding domains mediates a unique pattern of membrane localization. Specific membrane binding is retained by an N-terminal fragment (AR149 that corresponds to a naturally occurring splice variant termed DA-RAF2. AR149 colocalizes with ARF6 on tubular endosomes and has a dominant negative effect on endocytic trafficking. Moreover actin polymerization of yeast and mammalian cells is abolished. AR149/DA-RAF2 does not affect the internalization step of endocytosis, but trafficking to the recycling compartment. CONCLUSIONS/SIGNIFICANCE: A-RAF induced ERK activation is required for this step by activating ARF6, as A-RAF depletion or inhibition of the A-RAF controlled MEK-ERK cascade blocks recycling. These data led to a new model for A-RAF function in endocytic trafficking.

  5. Atmospheric blocking as a traffic jam in the jet stream

    Science.gov (United States)

    Nakamura, N.; Huang, S. Y.

    2017-12-01

    It is demonstrated using the ERA-Interim product that synoptic to intraseasonal variabilities of extratropical circulation in the boreal storm track regions are strongly affected by the zonal convergence of the column-integrated eastward flux of local wave activity (LWA). In particular, from the multi-year daily samples of LWA fluxes, we find that the wintertime zonal LWA flux in the jet exit regions tends to maximize for an intermediate value of column-averaged LWA. This is because an increasing LWA decelerates the zonal flow, eventually weakening the eastward advection of LWA. From theory we argue that large wave events on the decreasing side of the flux curve with increasing LWA cannot be maintained as a stable steady state. Consistent with this argument, observed states corresponding to that side of flux curve often exhibit local wave breaking and blocking events. A close parallelism exists for the traffic flow problem, in which the traffic flux (traffic density times traffic speed) is often observed to maximize for an intermediate value of traffic density. This is because the traffic speed is controlled not only by the imposed speed limit but also by the traffic density — an increasingly heavy traffic slows down the flow naturally and eventually decreases the flux. Once the flux starts to decrease with an increasing traffic density, a traffic jam kicks in suddenly (Lighthill and Whitham 1955, Richards 1956). The above idea is demonstrated by a simple conceptual model based on the equivalent barotropic PV contour design (Nakamura and Huang 2017, JAS), which predicts a threshold of blocking onset. The idea also suggests that the LWA that gives the `flux capacity,' i.e., the maximum LWA flux at a given location, is a useful predictor of local wave breaking/block formation.

  6. Exosomes surf on filopodia to enter cells at endocytic hot spots, traffic within endosomes, and are targeted to the ER.

    Science.gov (United States)

    Heusermann, Wolf; Hean, Justin; Trojer, Dominic; Steib, Emmanuelle; von Bueren, Stefan; Graff-Meyer, Alexandra; Genoud, Christel; Martin, Katrin; Pizzato, Nicolas; Voshol, Johannes; Morrissey, David V; Andaloussi, Samir E L; Wood, Matthew J; Meisner-Kober, Nicole C

    2016-04-25

    Exosomes are nanovesicles released by virtually all cells, which act as intercellular messengers by transfer of protein, lipid, and RNA cargo. Their quantitative efficiency, routes of cell uptake, and subcellular fate within recipient cells remain elusive. We quantitatively characterize exosome cell uptake, which saturates with dose and time and reaches near 100% transduction efficiency at picomolar concentrations. Highly reminiscent of pathogenic bacteria and viruses, exosomes are recruited as single vesicles to the cell body by surfing on filopodia as well as filopodia grabbing and pulling motions to reach endocytic hot spots at the filopodial base. After internalization, exosomes shuttle within endocytic vesicles to scan the endoplasmic reticulum before being sorted into the lysosome as their final intracellular destination. Our data quantify and explain the efficiency of exosome internalization by recipient cells, establish a new parallel between exosome and virus host cell interaction, and suggest unanticipated routes of subcellular cargo delivery. © 2016 Heusermann et al.

  7. Bifurcation of the endocytic pathway into Rab5-dependent and -independent transport to the vacuole

    Science.gov (United States)

    Toshima, Junko Y.; Nishinoaki, Show; Sato, Yoshifumi; Yamamoto, Wataru; Furukawa, Daiki; Siekhaus, Daria Elisabeth; Sawaguchi, Akira; Toshima, Jiro

    2014-03-01

    The yeast Rab5 homologue, Vps21p, is known to be involved both in the vacuolar protein sorting (VPS) pathway from the trans-Golgi network to the vacuole, and in the endocytic pathway from the plasma membrane to the vacuole. However, the intracellular location at which these two pathways converge remains unclear. In addition, the endocytic pathway is not completely blocked in yeast cells lacking all Rab5 genes, suggesting the existence of an unidentified route that bypasses the Rab5-dependent endocytic pathway. Here we show that convergence of the endocytic and VPS pathways occurs upstream of the requirement for Vps21p in these pathways. We also identify a previously unidentified endocytic pathway mediated by the AP-3 complex. Importantly, the AP-3-mediated pathway appears mostly intact in Rab5-disrupted cells, and thus works as an alternative route to the vacuole/lysosome. We propose that the endocytic traffic branches into two routes to reach the vacuole: a Rab5-dependent VPS pathway and a Rab5-independent AP-3-mediated pathway.

  8. Effects of PDT on the endocytic pathway

    Science.gov (United States)

    Kessel, David

    2010-02-01

    Two lines of evidence point to an early effect of photodamage on membrane trafficking. [1] Internalization of a fluorescent probe for hydrophobic membrane loci was impaired by prior photodamage. [2] Interference with the endocytic pathway by the PI-3 kinase antagonist wortmannin led to accumulation of cytoplasmic vacuoles suggesting a block in the recycling of plasma membrane components. Prior photodamage blocked this pathway so that no vacuoles were formed upon exposure of cells to wortmannin. In a murine hepatoma line, the endocytic pathway was preferentially sensitive to lysosomal photodamage. The role of photodamage to the endocytic pathway as a factor in PDT efficacy remains to be assessed.

  9. Traffic

    International Nuclear Information System (INIS)

    Lichtblau, G.

    2001-01-01

    This chapter deals with passenger and freight traffic, public and private transportation, traffic related environmental impacts, future developments, traffic indicators, regional traffic planning, health costs due to road traffic related air pollution, noise pollution, measures and regulations for traffic control and fuels for traffic. In particular energy consumption, energy efficiency, pollutant emissions ( CO 2 , SO 2 , NO x , HC, CO, N 2 O, NH 3 and particulates) and environmental effects of the different types of traffic and different types of fuels are compared and studied. Legal regulations and measures for an effective traffic control are discussed. (a.n.)

  10. Uveal melanoma cell-based vaccines express MHC II molecules that traffic via the endocytic and secretory pathways and activate CD8+ cytotoxic, tumor-specific T cells.

    Science.gov (United States)

    Bosch, Jacobus J; Iheagwara, Uzoma K; Reid, Sarah; Srivastava, Minu K; Wolf, Julie; Lotem, Michal; Ksander, Bruce R; Ostrand-Rosenberg, Suzanne

    2010-01-01

    We are exploring cell-based vaccines as a treatment for the 50% of patients with large primary uveal melanomas who develop lethal metastatic disease. MHC II uveal melanoma vaccines are MHC class I(+) uveal melanoma cells transduced with CD80 genes and MHC II genes syngeneic to the recipient. Previous studies demonstrated that the vaccines activate tumor-specific CD4(+) T cells from patients with metastatic uveal melanoma. We have hypothesized that vaccine potency is due to the absence of the MHC II-associated invariant chain (Ii). In the absence of Ii, newly synthesized MHC II molecules traffic intracellularly via a non-traditional pathway where they encounter and bind novel tumor peptides. Using confocal microscopy, we now confirm this hypothesis and demonstrate that MHC II molecules are present in both the endosomal and secretory pathways in vaccine cells. We also demonstrate that uveal melanoma MHC II vaccines activate uveal melanoma-specific, cytolytic CD8(+) T cells that do not lyse normal fibroblasts or other tumor cells. Surprisingly, the CD8(+) T cells are cytolytic for HLA-A syngeneic and MHC I-mismatched uveal melanomas. Collectively, these studies demonstrate that MHC II uveal melanoma vaccines are potent activators of tumor-specific CD4(+) and CD8(+) T cells and suggest that the non-conventional intracellular trafficking pattern of MHC II may contribute to their enhanced immunogenicity. Since MHC I compatibility is unnecessary for the activation of cytolytic CD8(+) T cells, the vaccines could be used in uveal melanoma patients without regard to MHC I genotype.

  11. Endocytic collagen degradation

    DEFF Research Database (Denmark)

    Madsen, Daniel H.; Jürgensen, Henrik J.; Ingvarsen, Signe Ziir

    2012-01-01

    Fibrosis of the liver and its end-stage, cirrhosis, represent major health problems worldwide. In these fibrotic conditions, activated fibroblasts and hepatic stellate cells display a net deposition of collagen. This collagen deposition is a major factor leading to liver dysfunction, thus making...... it crucially important to understand both the collagen synthesis and turnover mechanisms in this condition. Here we show that the endocytic collagen receptor, uPARAP/Endo180, is a major determinant in governing the balance between collagen deposition and degradation. Cirrhotic human livers displayed a marked...... importance of this collagen receptor in vivo, liver fibrosis was induced in uPARAP/Endo180-deficient mice and littermate wild-type mice by chronic CCl(4) administration. A strong up-regulation of uPARAP/Endo180 was observed in wild-type mice, and a quantitative comparison of collagen deposits in the two...

  12. Numb is an endocytic protein

    DEFF Research Database (Denmark)

    Santolini, E; Puri, C; Salcini, A E

    2000-01-01

    Numb is a protein that in Drosophila determines cell fate as a result of its asymmetric partitioning at mitosis. The function of Numb has been linked to its ability to bind and to biologically antagonize Notch, a membrane receptor that also specifies cell fate. The biochemical mechanisms underlying......15, a component of the endocytic machinery. Here, we demonstrate that Numb is an endocytic protein. We found that Numb localizes to endocytic organelles and is cotrafficked with internalizing receptors. Moreover, it associates with the appendage domain of alpha adaptin, a subunit of AP2, a major...

  13. The Vacuolar Import and Degradation Pathway Merges with the Endocytic Pathway to Deliver Fructose-1,6-bisphosphatase to the Vacuole for Degradation*

    Science.gov (United States)

    Brown, C. Randell; Wolfe, Allison B.; Cui, Dongying; Chiang, Hui-Ling

    2008-01-01

    The gluconeogenic enzyme fructose-1,6-bisphosphatase (FBPase) is degraded in the vacuole when glucose is added to glucose-starved cells. Before it is delivered to the vacuole, however, FBPase is imported into intermediate carriers called Vid (vacuole import and degradation) vesicles. Here, using biochemical and genetic approaches, we identified a requirement for SEC28 in FBPase degradation. SEC28 encodes the ε-COP subunit of COPI (coat protein complex I) coatomer proteins. When SEC28 and other coatomer genes were mutated, FBPase degradation was defective and FBPase association with Vid vesicles was impaired. Coatomer proteins were identified as components of Vid vesicles, and they formed a protein complex with a Vid vesicle-specific protein, Vid24p. Furthermore, Vid24p association with Vid vesicles was impaired when coatomer genes were mutated. Kinetic studies indicated that Sec28p traffics to multiple locations. Sec28p was in Vid vesicles, endocytic compartments, and the vacuolar membrane in various mutants that block the FBPase degradation pathway. Sec28p was also found in vesicles adjacent to the vacuolar membrane in the ret2-1 coatomer mutant. We propose that Sec28p resides in Vid vesicles, and these vesicles converge with the endocytic pathway. After fusion, Sec28p is distributed on the vacuolar membrane, where it concentrates on vesicles that pinch off from this organelle. FBPase also utilizes the endocytic pathway for transport to the vacuole, as demonstrated by its presence in endocytic compartments in the Δvph1 mutant. Taken together, our results indicate a strong connection between the Vid trafficking pathway and the endocytic pathway. PMID:18660504

  14. The vacuolar import and degradation pathway merges with the endocytic pathway to deliver fructose-1,6-bisphosphatase to the vacuole for degradation.

    Science.gov (United States)

    Brown, C Randell; Wolfe, Allison B; Cui, Dongying; Chiang, Hui-Ling

    2008-09-19

    The gluconeogenic enzyme fructose-1,6-bisphosphatase (FBPase) is degraded in the vacuole when glucose is added to glucose-starved cells. Before it is delivered to the vacuole, however, FBPase is imported into intermediate carriers called Vid (vacuole import and degradation) vesicles. Here, using biochemical and genetic approaches, we identified a requirement for SEC28 in FBPase degradation. SEC28 encodes the epsilon-COP subunit of COPI (coat protein complex I) coatomer proteins. When SEC28 and other coatomer genes were mutated, FBPase degradation was defective and FBPase association with Vid vesicles was impaired. Coatomer proteins were identified as components of Vid vesicles, and they formed a protein complex with a Vid vesicle-specific protein, Vid24p. Furthermore, Vid24p association with Vid vesicles was impaired when coatomer genes were mutated. Kinetic studies indicated that Sec28p traffics to multiple locations. Sec28p was in Vid vesicles, endocytic compartments, and the vacuolar membrane in various mutants that block the FBPase degradation pathway. Sec28p was also found in vesicles adjacent to the vacuolar membrane in the ret2-1 coatomer mutant. We propose that Sec28p resides in Vid vesicles, and these vesicles converge with the endocytic pathway. After fusion, Sec28p is distributed on the vacuolar membrane, where it concentrates on vesicles that pinch off from this organelle. FBPase also utilizes the endocytic pathway for transport to the vacuole, as demonstrated by its presence in endocytic compartments in the Deltavph1 mutant. Taken together, our results indicate a strong connection between the Vid trafficking pathway and the endocytic pathway.

  15. Aberrant trafficking of NSCLC-associated EGFR mutants through the endocytic recycling pathway promotes interaction with Src@

    Directory of Open Access Journals (Sweden)

    Band Vimla

    2009-11-01

    Full Text Available Abstract Background Epidermal growth factor receptor (EGFR controls a wide range of cellular processes, and altered EGFR signaling contributes to human cancer. EGFR kinase domain mutants found in non-small cell lung cancer (NSCLC are constitutively active, a trait critical for cell transformation through activation of downstream pathways. Endocytic trafficking of EGFR is a major regulatory mechanism as ligand-induced lysosomal degradation results in termination of signaling. While numerous studies have examined mutant EGFR signaling, the endocytic traffic of mutant EGFR within the NSCLC milieu remains less clear. Results This study shows that mutant EGFRs in NSCLC cell lines are constitutively endocytosed as shown by their colocalization with the early/recycling endosomal marker transferrin and the late endosomal/lysosomal marker LAMP1. Notably, mutant EGFRs, but not the wild-type EGFR, show a perinuclear accumulation and colocalization with recycling endosomal markers such as Rab11 and EHD1 upon treatment of cells with endocytic recycling inhibitor monensin, suggesting that mutant EGFRs preferentially traffic through the endocytic recycling compartments. Importantly, monensin treatment enhanced the mutant EGFR association and colocalization with Src, indicating that aberrant transit through the endocytic recycling compartment promotes mutant EGFR-Src association. Conclusion The findings presented in this study show that mutant EGFRs undergo aberrant traffic into the endocytic recycling compartment which allows mutant EGFRs to engage in a preferential interaction with Src, a critical partner for EGFR-mediated oncogenesis.

  16. Endocytic pathways mediating oligomeric Aβ42 neurotoxicity

    Directory of Open Access Journals (Sweden)

    Laxton Kevin

    2010-05-01

    Full Text Available Abstract Background One pathological hallmark of Alzheimer's disease (AD is amyloid plaques, composed primarily of amyloid-β peptide (Aβ. Over-production or diminished clearance of the 42 amino acid form of Aβ (Aβ42 in the brain leads to accumulation of soluble Aβ and plaque formation. Soluble oligomeric Aβ (oAβ has recently emerged to be as a likely proximal cause of AD. Results Here we demonstrate that endocytosis is critical in mediating oAβ42-induced neurotoxicity and intraneuronal accumulation of Aβ. Inhibition of clathrin function either with a pharmacological inhibitor, knock-down of clathrin heavy chain expression, or expression of the dominant-negative mutant of clathrin-assembly protein AP180 did not block oAβ42-induced neurotoxicity or intraneuronal accumulation of Aβ. However, inhibition of dynamin and RhoA by expression of dominant negative mutants reduced neurotoxicity and intraneuronal Aβ accumulation. Pharmacologic inhibition of the dynamin-mediated endocytic pathway by genistein also reduced neurotoxicity. Conclusions These data suggest that dynamin-mediated and RhoA-regulated endocytosis are integral steps for oligomeric Aβ42-induced neurotoxicity and intraneuronal Aβ accumulation.

  17. Identifying factors affecting the safety of mid-block bicycle lanes considering mixed 2-wheeled traffic flow.

    Science.gov (United States)

    Bai, Lu; Chan, Ching-Yao; Liu, Pan; Xu, Chengcheng

    2017-10-03

    Electric bikes (e-bikes) have been one of the fastest growing trip modes in Southeast Asia over the past 2 decades. The increasing popularity of e-bikes raised some safety concerns regarding urban transport systems. The primary objective of this study was to identify whether and how the generalized linear regression model (GLM) could be used to relate cyclists' safety with various contributing factors when riding in a mid-block bike lane. The types of 2-wheeled vehicles in the study included bicycle-style electric bicycles (BSEBs), scooter-style electric bicycles (SSEBs), and regular bicycles (RBs). Traffic conflict technology was applied as a surrogate measure to evaluate the safety of 2-wheeled vehicles. The safety performance model was developed by adopting a generalized linear regression model for relating the frequency of rear-end conflicts between e-bikes and regular bikes to the operating speeds of BSEBs, SSEBs, and RBs in mid-block bike lanes. The frequency of rear-end conflicts between e-bikes and bikes increased with an increase in the operating speeds of e-bikes and the volume of e-bikes and bikes and decreased with an increase in the width of bike lanes. The large speed difference between e-bikes and bikes increased the frequency of rear-end conflicts between e-bikes and bikes in mid-block bike lanes. A 1% increase in the average operating speed of e-bikes would increase the expected number of rear-end conflicts between e-bikes and bikes by 1.48%. A 1% increase in the speed difference between e-bikes and bikes would increase the expected number of rear-end conflicts between e-bikes/bikes by 0.16%. The conflict frequency in mid-block bike lanes can be modeled using generalized linear regression models. The factors that significantly affected the frequency of rear-end conflicts included the operating speeds of e-bikes, the speed difference between e-bikes and regular bikes, the volume of e-bikes, the volume of bikes, and the width of bike lanes. The

  18. Anti-α4 antibody treatment blocks virus traffic to the brain and gut early, and stabilizes CNS injury late in infection.

    Directory of Open Access Journals (Sweden)

    Jennifer H Campbell

    2014-12-01

    Full Text Available Four SIV-infected monkeys with high plasma virus and CNS injury were treated with an anti-α4 blocking antibody (natalizumab once a week for three weeks beginning on 28 days post-infection (late. Infection in the brain and gut were quantified, and neuronal injury in the CNS was assessed by MR spectroscopy, and compared to controls with AIDS and SIV encephalitis. Treatment resulted in stabilization of ongoing neuronal injury (NAA/Cr by 1H MRS, and decreased numbers of monocytes/macrophages and productive infection (SIV p28+, RNA+ in brain and gut. Antibody treatment of six SIV infected monkeys at the time of infection (early for 3 weeks blocked monocyte/macrophage traffic and infection in the CNS, and significantly decreased leukocyte traffic and infection in the gut. SIV - RNA and p28 was absent in the CNS and the gut. SIV DNA was undetectable in brains of five of six early treated macaques, but proviral DNA in guts of treated and control animals was equivalent. Early treated animals had low-to-no plasma LPS and sCD163. These results support the notion that monocyte/macrophage traffic late in infection drives neuronal injury and maintains CNS viral reservoirs and lesions. Leukocyte traffic early in infection seeds the CNS with virus and contributes to productive infection in the gut. Leukocyte traffic early contributes to gut pathology, bacterial translocation, and activation of innate immunity.

  19. Endocytic reawakening of motility in jammed epithelia

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    Malinverno, Chiara; Corallino, Salvatore; Giavazzi, Fabio; Bergert, Martin; Li, Qingsen; Leoni, Marco; Disanza, Andrea; Frittoli, Emanuela; Oldani, Amanda; Martini, Emanuele; Lendenmann, Tobias; Deflorian, Gianluca; Beznoussenko, Galina V.; Poulikakos, Dimos; Ong, Kok Haur; Uroz, Marina; Trepat, Xavier; Parazzoli, Dario; Maiuri, Paolo; Yu, Weimiao; Ferrari, Aldo; Cerbino, Roberto; Scita, Giorgio

    2017-05-01

    Dynamics of epithelial monolayers has recently been interpreted in terms of a jamming or rigidity transition. How cells control such phase transitions is, however, unknown. Here we show that RAB5A, a key endocytic protein, is sufficient to induce large-scale, coordinated motility over tens of cells, and ballistic motion in otherwise kinetically arrested monolayers. This is linked to increased traction forces and to the extension of cell protrusions, which align with local velocity. Molecularly, impairing endocytosis, macropinocytosis or increasing fluid efflux abrogates RAB5A-induced collective motility. A simple model based on mechanical junctional tension and an active cell reorientation mechanism for the velocity of self-propelled cells identifies regimes of monolayer dynamics that explain endocytic reawakening of locomotion in terms of a combination of large-scale directed migration and local unjamming. These changes in multicellular dynamics enable collectives to migrate under physical constraints and may be exploited by tumours for interstitial dissemination.

  20. Inactivation of Tor proteins affects the dynamics of endocytic proteins ...

    Indian Academy of Sciences (India)

    Tor2 is an activator of the Rom2/Rho1 pathway that regulates -factor internalization. Since the recruitment of endocytic proteins such as actin-binding proteins and the amphiphysins precedes the internalization of -factor, we hypothesized that loss of Tor function leads to an alteration in the dynamics of the endocytic ...

  1. The HPV16 E5 oncogene inhibits endocytic trafficking

    DEFF Research Database (Denmark)

    Thomsen, P; van Deurs, B; Norrild, B

    2000-01-01

    The small hydrophobic E5 protein of Human Papillomavirus type 16 (HPV16) binds to the 16-kDa subunit of the V-H+-ATPase. This binding has been suggested to interfere with acidification of late endocytic structures. We here used video microscopy, ratio imaging and confocal microscopy of living C127...... perturbs trafficking from early to late endocytic structures rather than acidification....

  2. Endocytic recycling protein EHD1 regulates primary cilia morphogenesis and SHH signaling during neural tube development.

    Science.gov (United States)

    Bhattacharyya, Sohinee; Rainey, Mark A; Arya, Priyanka; Dutta, Samikshan; George, Manju; Storck, Matthew D; McComb, Rodney D; Muirhead, David; Todd, Gordon L; Gould, Karen; Datta, Kaustubh; Gelineau-van Waes, Janee; Band, Vimla; Band, Hamid

    2016-02-17

    Members of the four-member C-terminal EPS15-Homology Domain-containing (EHD) protein family play crucial roles in endocytic recycling of cell surface receptors from endosomes to the plasma membrane. In this study, we show that Ehd1 gene knockout in mice on a predominantly B6 background is embryonic lethal. Ehd1-null embryos die at mid-gestation with a failure to complete key developmental processes including neural tube closure, axial turning and patterning of the neural tube. We found that Ehd1-null embryos display short and stubby cilia on the developing neuroepithelium at embryonic day 9.5 (E9.5). Loss of EHD1 also deregulates the ciliary SHH signaling with Ehd1-null embryos displaying features indicative of increased SHH signaling, including a significant downregulation in the formation of the GLI3 repressor and increase in the ventral neuronal markers specified by SHH. Using Ehd1-null MEFS we found that EHD1 protein co-localizes with the SHH receptor Smoothened in the primary cilia upon ligand stimulation. Under the same conditions, EHD1 was shown to co-traffic with Smoothened into the developing primary cilia and we identify EHD1 as a direct binding partner of Smoothened. Overall, our studies identify the endocytic recycling regulator EHD1 as a novel regulator of the primary cilium-associated trafficking of Smoothened and Hedgehog signaling.

  3. Chemical genetic screening identifies sulfonamides that raise organellar pH and interfere with membrane traffic.

    Science.gov (United States)

    Nieland, Thomas J F; Feng, Yan; Brown, Jing Xu; Chuang, Tuan Daniel; Buckett, Peter D; Wang, Jin; Xie, Xiao-Song; McGraw, Timothy E; Kirchhausen, Tomas; Wessling-Resnick, Marianne

    2004-07-01

    Chemical genetics seeks to identify small molecules that afford functional dissection of cell biological pathways. Previous screens for small molecule inhibitors of exocytic membrane traffic yielded the identification and characterization of several compounds that block traffic from the Golgi to the cell surface as well as transport from the endoplasmic reticulum to the Golgi network [Feng et al. Proc Natl Acad Sci USA 2003;100:6469-6474; Yarrow et al. Comb Chem High Throughput Screen 2003;6:279-286; Feng et al. EMBO Reports 2004: in press]. Here, we screened these inhibitors for potential effects on endocytic membrane traffic. Two structurally related sulfonamides were found to be potent and reversible inhibitors of transferrin-mediated iron uptake. These inhibitors do not block endoplasmic reticulum-to-Golgi transport, but do disrupt Golgi-to-cell surface traffic. The compounds are members of a novel class of sulfonamides that elevate endosomal and lysosomal pH, down-regulate cell surface receptors, and impair recycling of internalized transferrin receptors to the plasma membrane. In vitro experiments revealed that the sulfonamides directly inhibit adenosine triphosphate (ATP) hydrolysis by the V-ATPase and that they also possess a potent proton ionophore activity. While maintenance of organellar pH is known to be a critical factor in both endocytosis and exocytosis, the precise role of acidification, beyond the uncoupling of ligands from their receptors, remains largely unknown. Identification of this novel class of sulfonamide inhibitors provides new chemical tools to better understand the function of organelle pH in membrane traffic and the activity of V-ATPases in particular.

  4. Intersection of Endocytic and Exocytic Systems in Toxoplasma gondii.

    Science.gov (United States)

    McGovern, Olivia L; Rivera-Cuevas, Yolanda; Kannan, Geetha; Narwold, Andrew; Carruthers, Vern B

    2018-02-13

    Host cytosolic proteins are endocytosed by Toxoplasma gondii and degraded in its lysosome-like compartment, the VAC, but the dynamics and route of endocytic trafficking remain undefined. Conserved endocytic components and plant-like features suggest T. gondii endocytic trafficking involves transit through early and late endosome-like compartments (ELCs) and potentially the trans-Golgi network (TGN) as in plants. However, exocytic trafficking to regulated secretory organelles, micronemes and rhoptries, also proceeds through ELCs and requires classical endocytic components including a dynamin-related protein, DrpB. Here we show that host cytosolic proteins are endocytosed within 7 min post-invasion, trafficked through ELCs en route to the VAC, and degraded within 30 min. We could not definitively interpret if ingested protein is trafficked through the TGN. We also found that parasites ingest material from the host cytosol throughout the parasite cell cycle. Ingested host proteins colocalize with immature microneme proteins, proM2AP and proMIC5, in transit to the micronemes, but not with the immature rhoptry protein proRON4, indicating that endocytic trafficking of ingested protein intersects with exocytic trafficking of microneme proteins. Finally, we show that conditional expression of a DrpB dominant negative mutant increases T. gondii ingestion of host-derived proteins, suggesting that DrpB is not required for parasite endocytosis. This article is protected by copyright. All rights reserved.

  5. Membrane order in the plasma membrane and endocytic recycling compartment.

    Science.gov (United States)

    Iaea, David B; Maxfield, Frederick R

    2017-01-01

    The cholesterol content of membranes plays an important role in organizing membranes for signal transduction and protein trafficking as well as in modulating the biophysical properties of membranes. While the properties of model or isolated membranes have been extensively studied, there has been little evaluation of internal membranes in living cells. Here, we use a Nile Red based probe, NR12S, and ratiometric live cell imaging, to analyze the membrane order of the plasma membrane and endocytic recycling compartment. We find that after a brief incubation to allow endocytosis, NR12S is distributed between the plasma membrane and the endocytic recycling compartment. The NR12S reports that the endocytic recycling compartment is more highly ordered than the plasma membrane. We also find that the plasma membrane and the endocytic recycling compartment are differentially affected by altering cellular cholesterol levels. The membrane order of the plasma membrane, but not the endocytic recycling compartment, is altered significantly when cellular cholesterol content is increased or decreased by 20%. These results demonstrate that changes in cellular cholesterol differentially alter membrane order within different organelles.

  6. Traffic theory

    National Research Council Canada - National Science Library

    Gazis, Denos C

    2002-01-01

    ... of traffic signal settings The vehicle-actuated traffic signal 87 89 77 CHAPTER 3. TRAFFIC CONTROL 101 Objectives of Traffic Control 103 Single, Isolated Intersection 105 Synchronization Scheme...

  7. Inactivation of Tor proteins affects the dynamics of endocytic proteins ...

    Indian Academy of Sciences (India)

    2013-04-26

    Apr 26, 2013 ... Tor2 is an activator of the Rom2/Rho1 pathway that regulates α-factor internalization. Since the recruitment of endocytic ... stimulate actin polymerization, which facilitates the invagi- nation of the plasma membrane ... the stage was maintained at 30°C or 38°C. Images were focused at an equatorial plane of ...

  8. The HPV16 E5 oncogene inhibits endocytic trafficking

    DEFF Research Database (Denmark)

    Thomsen, P; van Deurs, B; Norrild, B

    2000-01-01

    The small hydrophobic E5 protein of Human Papillomavirus type 16 (HPV16) binds to the 16-kDa subunit of the V-H+-ATPase. This binding has been suggested to interfere with acidification of late endocytic structures. We here used video microscopy, ratio imaging and confocal microscopy of living C127...

  9. Melanin Transferred to Keratinocytes Resides in Nondegradative Endocytic Compartments.

    Science.gov (United States)

    Correia, Maria S; Moreiras, Hugo; Pereira, Francisco J C; Neto, Matilde V; Festas, Tiago C; Tarafder, Abul K; Ramalho, José S; Seabra, Miguel C; Barral, Duarte C

    2018-03-01

    Melanin transfer from melanocytes to keratinocytes and subsequent accumulation in the supranuclear region is a critical process in skin pigmentation and protection against UVR. We have previously proposed that the main mode of transfer between melanocytes and keratinocytes is through exo/endocytosis of the melanosome core, termed melanocore. In this study, we developed an in vitro uptake assay using melanocores secreted by melanocytes. We show that the uptake of melanocores, but not melanosomes, by keratinocytes is protease-activated receptor-2-dependent. Furthermore, we found that the silencing of the early endocytic regulator Rab5b, but not the late endocytic regulators Rab7a or Rab9a, significantly impairs melanocore uptake by keratinocytes. After uptake, we observed that melanin accumulates in compartments that are positive for both early and late endocytic markers. We found that melanin does not localize to either highly degradative or acidic organelles, as assessed by LysoTracker and DQ-BSA staining, despite the abundance of these types of organelles within keratinocytes. Therefore, we propose that melanocore uptake leads to storage of melanin within keratinocytes in hybrid endocytic compartments that are not highly acidic or degradative. By avoiding lysosomal degradation, these specialized endosomes may allow melanin to persist within keratinocytes for long periods. Copyright © 2017 The Authors. Published by Elsevier Inc. All rights reserved.

  10. Effector protein translocation by the Coxiella burnetii Dot/Icm type IV secretion system requires endocytic maturation of the pathogen-occupied vacuole.

    Directory of Open Access Journals (Sweden)

    Hayley J Newton

    Full Text Available The human pathogen Coxiella burnetii encodes a type IV secretion system called Dot/Icm that is essential for intracellular replication. The Dot/Icm system delivers bacterial effector proteins into the host cytosol during infection. The effector proteins delivered by C. burnetii are predicted to have important functions during infection, but when these proteins are needed during infection has not been clearly defined. Here, we use a reporter system consisting of fusion proteins that have a β-lactamase enzyme (BlaM fused to C. burnetii effector proteins to study protein translocation by the Dot/Icm system. Translocation of BlaM fused to the effector proteins CBU0077, CBU1823 and CBU1524 was not detected until 8-hours after infection of HeLa cells, which are permissive for C. burnetii replication. Translocation of these effector fusion proteins by the Dot/Icm system required acidification of the Coxiella-containing vacuole. Silencing of the host genes encoding the membrane transport regulators Rab5 or Rab7 interfered with effector translocation, which indicates that effectors are not translocated until bacteria traffic to a late endocytic compartment in the host cell. Similar requirements for effector translocation were discerned in bone marrow macrophages derived from C57BL/6 mice, which are primary cells that restrict the intracellular replication of C. burnetii. In addition to requiring endocytic maturation of the vacuole for Dot/Icm-mediated translocation of effectors, bacterial transcription was required for this process. Thus, translocation of effector proteins by the C. burnetii Dot/Icm system occurs after acidification of the CCV and maturation of this specialized organelle to a late endocytic compartment. This indicates that creation of the specialized vacuole in which C. burnetii replicates represents a two-stage process mediated initially by host factors that regulate endocytic maturation and then by bacterial effectors delivered into

  11. Cellular endocytic compartment localization of expressed canine CD1 molecules.

    Science.gov (United States)

    Schjaerff, Mette; Keller, Stefan M; Affolter, Verena K; Kristensen, Annemarie T; Moore, Peter F

    2016-12-01

    CD1 molecules are glycoproteins present primarily on dendritic cells (DCs), which recognize and present a variety of foreign- and self-lipid antigens to T-cells. Humans have five different CD1 isoforms that survey distinct cellular compartments allowing for recognition of a large repertoire of lipids. The canine CD1 family consists of seven functional CD1 molecules (canine CD1a2, CD1a6, CD1a8, CD1a9, CD1b, CD1c and CD1e) and one presumed non-functional isoform (canine CD1d) due to a disrupted gene structure. The aim of this study was to describe in vitro steady-state localization ptterns of canine CD1 isoforms and their correlation with endocytic organelles. GFP-fused canine CD1 293T cell transfectants were stained with markers for early endocytic compartments (EEA-1) and late endocytic/lysosomal compartments (LAMP-1), respectively, and analyzed by confocal microscopy. Canine CD1a molecules localized to the plasma membrane and partially to the early endocytic compartment, but not to late endosomes or lysosomes. In contrast, canine CD1b was highly associated with late endosomal/lysosomal compartments and showed a predominant intracellular expression pattern. Canine CD1c protein expression localized more promiscuously to both the early endosomal compartments and the late endosomal/lysosomal compartments. The canine CD1e molecule showed a strictly intracellular expression with a partial overlap with late endosomal/lysosomal compartments. Lastly, canine CD1d was expressed abnormally showing only a diminished GFP expression. In conclusion, canine CD1 transfectants show distinct localization patterns that are similar to human CD1 proteins with the exception of the canine CD1d isoform, which most likely is non-functional. These findings imply that canine CD1 localization overall resembles human CD1 trafficking patterns. This knowledge is important for the understanding of lipid antigen-receptor immunity in the dog. Copyright © 2016. Published by Elsevier B.V.

  12. Megalin functions as an endocytic sonic hedgehog receptor.

    Science.gov (United States)

    McCarthy, Robert A; Barth, Jeremy L; Chintalapudi, Mastan R; Knaak, Christian; Argraves, W Scott

    2002-07-12

    Embryos deficient in the morphogen Sonic hedgehog (Shh) or the endocytic receptor megalin exhibit common neurodevelopmental abnormalities. Therefore, we have investigated the possibility that a functional relationship exists between the two proteins. During embryonic development, megalin was found to be expressed along the apical surfaces of neuroepithelial cells and was coexpressed with Shh in the ventral floor plate of the neural tube. Using enzyme-linked immunosorbent assay, homologous ligand displacement, and surface plasmon resonance techniques, it was found that the amino-terminal fragment of Shh (N-Shh) bound to megalin with high affinity. Megalin-expressing cells internalized N-Shh through a mechanism that was inhibited by antagonists of megalin, viz. anti-receptor-associated protein and anti-megalin antibodies. Heparin also inhibited N-Shh endocytosis, implicating proteoglycans in the internalization process, as has been described for other megalin ligands. Use of chloroquine to inhibit lysosomal proteinase activity showed that N-Shh endocytosed via megalin was not efficiently targeted to the lysosomes for degradation. The ability of megalin-internalized N-Shh to bypass lysosomes may relate to the finding that the interaction between N-Shh and megalin was resistant to dissociation with low pH. Together, these findings show that megalin is an efficient endocytic receptor for N-Shh. Furthermore, they implicate megalin as a new regulatory component of the Shh signaling pathway.

  13. Salicylic acid interferes with clathrin-mediated endocytic protein trafficking.

    Science.gov (United States)

    Du, Yunlong; Tejos, Ricardo; Beck, Martina; Himschoot, Ellie; Li, Hongjiang; Robatzek, Silke; Vanneste, Steffen; Friml, Jirí

    2013-05-07

    Removal of cargos from the cell surface via endocytosis is an efficient mechanism to regulate activities of plasma membrane (PM)-resident proteins, such as receptors or transporters. Salicylic acid (SA) is an important plant hormone that is traditionally associated with pathogen defense. Here, we describe an unanticipated effect of SA on subcellular endocytic cycling of proteins. Both exogenous treatments and endogenously enhanced SA levels repressed endocytosis of different PM proteins. The SA effect on endocytosis did not involve transcription or known components of the SA signaling pathway for transcriptional regulation. SA likely targets an endocytic mechanism that involves the coat protein clathrin, because SA interfered with the clathrin incidence at the PM and clathrin-deficient mutants were less sensitive to the impact of SA on the auxin distribution and root bending during the gravitropic response. By contrast, SA did not affect the ligand-induced endocytosis of the flagellin sensing2 (FLS2) receptor during pathogen responses. Our data suggest that the established SA impact on transcription in plant immunity and the nontranscriptional effect of SA on clathrin-mediated endocytosis are independent mechanisms by which SA regulates distinct aspects of plant physiology.

  14. Pre-cancerous changes in urothelial endocytic vesicle leakage, fatty acid composition, and As and associated element concentrations after arsenic exposure

    International Nuclear Information System (INIS)

    Grasso, E.J.; Bongiovanni, G.A.; Perez, R.D.; Calderon, R.O.

    2011-01-01

    The urothelium covering the luminal surface of the urinary bladder has developed an efficient permeability barrier that protects it against the back-flow of toxins eliminated in the urine. The subapical endocytic vesicles containing the urinary bladder fluid phase are formed during the micturition cycle by endocytosis processes of the superficial cells. In normal conditions, the permeability barrier of the endocytic vesicles blocks the passage of the fluid phase to the cellular cytoplasm and the fluid is recycled to the bladder lumen. The aim of this work was to investigate the alteration of the endocytic vesicle membrane permeability barrier to toxins such as iAs (inorganic arsenic) administered in drinking water. By using an induced endocytosis model and the fluorescence requenching technique, it is shown that the exposure of rats to ingestion of water containing iAs not only induced pre-cancerous morphological changes, but allowed the differential leakage of an endocytosed fluorescent marker, HPTS, and its quencher, DPX, (hydroxypyrene-1,3,6-trisulfonic acid and p-xylene-bis-pyridinium bromide, respectively) out of the vesicular lumen. The leakage of the cationic DPX was almost complete, while the release of the anionic HPTS molecule was partial and higher in arsenic-treated-rats than in controls. Such membrane alteration would allow the toxins to elude the permeability barrier and to leak out of the endocytic vesicles, thus establishing a 'bypass' to the permeability barrier. The retention of As in the urinary bladder, assessed by synchrotron radiation X-ray fluorescence spectrometry (SR-μXRF), was lower than the kidney accumulation of arsenic previously observed by our group and was accompanied by altered concentrations of K, Ca, Fe, Cu and Zn, all ions related to cellular metabolism. The results support the hypothesis that low amounts of endocytosed As can accumulate in the interior of the urothelial superficial cells and initiate the cytotoxic effects

  15. Proteomics of secretory and endocytic organelles in Giardia lamblia.

    Directory of Open Access Journals (Sweden)

    Petra B Wampfler

    Full Text Available Giardia lamblia is a flagellated protozoan enteroparasite transmitted as an environmentally resistant cyst. Trophozoites attach to the small intestine of vertebrate hosts and proliferate by binary fission. They access nutrients directly via uptake of bulk fluid phase material into specialized endocytic organelles termed peripheral vesicles (PVs, mainly on the exposed dorsal side. When trophozoites reach the G2/M restriction point in the cell cycle they can begin another round of cell division or encyst if they encounter specific environmental cues. They induce neogenesis of Golgi-like organelles, encystation-specific vesicles (ESVs, for regulated secretion of cyst wall material. PVs and ESVs are highly simplified and thus evolutionary diverged endocytic and exocytic organelle systems with key roles in proliferation and transmission to a new host, respectively. Both organelle systems physically and functionally intersect at the endoplasmic reticulum (ER which has catabolic as well as anabolic functions. However, the unusually high degree of sequence divergence in Giardia rapidly exhausts phylogenomic strategies to identify and characterize the molecular underpinnings of these streamlined organelles. To define the first proteome of ESVs and PVs we used a novel strategy combining flow cytometry-based organelle sorting with in silico filtration of mass spectrometry data. From the limited size datasets we retrieved many hypothetical but also known organelle-specific factors. In contrast to PVs, ESVs appear to maintain a strong physical and functional link to the ER including recruitment of ribosomes to organelle membranes. Overall the data provide further evidence for the formation of a cyst extracellular matrix with minimal complexity. The mass spectrometry proteomics data have been deposited to the ProteomeXchange Consortium with the dataset identifier PXD000694.

  16. Renal thrombotic microangiopathy in mice with combined deletion of endocytic recycling regulators EHD3 and EHD4.

    Directory of Open Access Journals (Sweden)

    Manju George

    2011-03-01

    Full Text Available Eps15 Homology Domain-containing 3 (EHD3, a member of the EHD protein family that regulates endocytic recycling, is the first protein reported to be specifically expressed in the glomerular endothelium in the kidney; therefore we generated Ehd3(-/- mice and assessed renal development and pathology. Ehd3(-/- animals showed no overt defects, and exhibited no proteinuria or glomerular pathology. However, as the expression of EHD4, a related family member, was elevated in the glomerular endothelium of Ehd3(-/- mice and suggested functional compensation, we generated and analyzed Ehd3(-/-; Ehd4(-/- mice. These mice were smaller, possessed smaller and paler kidneys, were proteinuric and died between 3-24 weeks of age. Detailed analyses of Ehd3(-/-; Ehd4(-/- kidneys demonstrated thrombotic microangiopathy (TMA-like glomerular lesions including thickening and duplication of glomerular basement membrane, endothelial swelling and loss of fenestrations. Other changes included segmental podocyte foot process effacement, mesangial interposition, and abnormal podocytic and mesangial marker expression. The glomerular lesions observed were strikingly similar to those seen in human pre-eclampsia and mouse models of reduced VEGF expression. As altered glomerular endothelial VEGFR2 expression and localization and increased apoptosis was observed in the absence of EHD3 and EHD4, we propose that EHD-mediated endocytic traffic of key surface receptors such as VEGFR2 is essential for physiological control of glomerular function. Furthermore, Ehd3(-/-; Ehd4(-/- mice provide a unique model to elucidate mechanisms of glomerular endothelial injury which is observed in a wide variety of human renal and extra-renal diseases.

  17. T47D Cells Expressing Myeloperoxidase Are Able to Process, Traffic and Store the Mature Protein in Lysosomes: Studies in T47D Cells Reveal a Role for Cys319 in MPO Biosynthesis that Precedes Its Known Role in Inter-Molecular Disulfide Bond Formation

    Science.gov (United States)

    Laura, Richard P.; Dong, David; Reynolds, Wanda F.; Maki, Richard A.

    2016-01-01

    Among the human heme-peroxidase family, myeloperoxidase (MPO) has a unique disulfide-linked oligomeric structure resulting from multi-step processing of the pro-protein monomer (proMPO) after it exits the endoplasmic reticulum (ER). Related family members undergo some, but not all, of the processing steps involved with formation of mature MPO. Lactoperoxidase has its pro-domain proteolytically removed and is a monomer in its mature form. Eosinophil peroxidase undergoes proteolytic removal of its pro-domain followed by proteolytic separation into heavy and light chains and is a heterodimer. However, only MPO undergoes both these proteolytic modifications and then is further oligomerized into a heterotetramer by a single inter-molecular disulfide bond. The details of how and where the post-ER processing steps of MPO occur are incompletely understood. We report here that T47D breast cancer cells stably transfected with an MPO expression plasmid are able to efficiently replicate all of the processing steps that lead to formation of the mature MPO heterotetramer. MPO also traffics to the lysosome granules of T47D cells where it accumulates, allowing in-depth immunofluorescent microscopy studies of MPO trafficking and storage for the first time. Using this novel cell model we show that formation of MPO’s single inter-molecular disulfide bond can occur normally in the absence of the proteolytic events that lead to separation of the MPO heavy and light chains. We further demonstrate that Cys319, which forms MPO’s unique inter-molecular disulfide bond, is important for events that precede this step. Mutation of this residue alters the glycosylation and catalytic activity of MPO and blocks its entry into the endocytic pathway where proteolytic processing and disulfide bonding occur. Finally, using the endocytic trafficking of lysosomal hydrolases as a guide, we investigate the role of candidate receptors in the endocytic trafficking of MPO. PMID:26890638

  18. T47D Cells Expressing Myeloperoxidase Are Able to Process, Traffic and Store the Mature Protein in Lysosomes: Studies in T47D Cells Reveal a Role for Cys319 in MPO Biosynthesis that Precedes Its Known Role in Inter-Molecular Disulfide Bond Formation.

    Directory of Open Access Journals (Sweden)

    Richard P Laura

    Full Text Available Among the human heme-peroxidase family, myeloperoxidase (MPO has a unique disulfide-linked oligomeric structure resulting from multi-step processing of the pro-protein monomer (proMPO after it exits the endoplasmic reticulum (ER. Related family members undergo some, but not all, of the processing steps involved with formation of mature MPO. Lactoperoxidase has its pro-domain proteolytically removed and is a monomer in its mature form. Eosinophil peroxidase undergoes proteolytic removal of its pro-domain followed by proteolytic separation into heavy and light chains and is a heterodimer. However, only MPO undergoes both these proteolytic modifications and then is further oligomerized into a heterotetramer by a single inter-molecular disulfide bond. The details of how and where the post-ER processing steps of MPO occur are incompletely understood. We report here that T47D breast cancer cells stably transfected with an MPO expression plasmid are able to efficiently replicate all of the processing steps that lead to formation of the mature MPO heterotetramer. MPO also traffics to the lysosome granules of T47D cells where it accumulates, allowing in-depth immunofluorescent microscopy studies of MPO trafficking and storage for the first time. Using this novel cell model we show that formation of MPO's single inter-molecular disulfide bond can occur normally in the absence of the proteolytic events that lead to separation of the MPO heavy and light chains. We further demonstrate that Cys319, which forms MPO's unique inter-molecular disulfide bond, is important for events that precede this step. Mutation of this residue alters the glycosylation and catalytic activity of MPO and blocks its entry into the endocytic pathway where proteolytic processing and disulfide bonding occur. Finally, using the endocytic trafficking of lysosomal hydrolases as a guide, we investigate the role of candidate receptors in the endocytic trafficking of MPO.

  19. T47D Cells Expressing Myeloperoxidase Are Able to Process, Traffic and Store the Mature Protein in Lysosomes: Studies in T47D Cells Reveal a Role for Cys319 in MPO Biosynthesis that Precedes Its Known Role in Inter-Molecular Disulfide Bond Formation.

    Science.gov (United States)

    Laura, Richard P; Dong, David; Reynolds, Wanda F; Maki, Richard A

    2016-01-01

    Among the human heme-peroxidase family, myeloperoxidase (MPO) has a unique disulfide-linked oligomeric structure resulting from multi-step processing of the pro-protein monomer (proMPO) after it exits the endoplasmic reticulum (ER). Related family members undergo some, but not all, of the processing steps involved with formation of mature MPO. Lactoperoxidase has its pro-domain proteolytically removed and is a monomer in its mature form. Eosinophil peroxidase undergoes proteolytic removal of its pro-domain followed by proteolytic separation into heavy and light chains and is a heterodimer. However, only MPO undergoes both these proteolytic modifications and then is further oligomerized into a heterotetramer by a single inter-molecular disulfide bond. The details of how and where the post-ER processing steps of MPO occur are incompletely understood. We report here that T47D breast cancer cells stably transfected with an MPO expression plasmid are able to efficiently replicate all of the processing steps that lead to formation of the mature MPO heterotetramer. MPO also traffics to the lysosome granules of T47D cells where it accumulates, allowing in-depth immunofluorescent microscopy studies of MPO trafficking and storage for the first time. Using this novel cell model we show that formation of MPO's single inter-molecular disulfide bond can occur normally in the absence of the proteolytic events that lead to separation of the MPO heavy and light chains. We further demonstrate that Cys319, which forms MPO's unique inter-molecular disulfide bond, is important for events that precede this step. Mutation of this residue alters the glycosylation and catalytic activity of MPO and blocks its entry into the endocytic pathway where proteolytic processing and disulfide bonding occur. Finally, using the endocytic trafficking of lysosomal hydrolases as a guide, we investigate the role of candidate receptors in the endocytic trafficking of MPO.

  20. The role of Ca2+ influx in endocytic vacuole formation in pancreatic acinar cells

    Science.gov (United States)

    Voronina, Svetlana; Collier, David; Chvanov, Michael; Middlehurst, Ben; Beckett, Alison J.; Prior, Ian A.; Criddle, David N.; Begg, Malcolm; Mikoshiba, Katsuhiko; Sutton, Robert; Tepikin, Alexei V.

    2014-01-01

    The inducers of acute pancreatitis trigger a prolonged increase in the cytosolic Ca2+ concentration ([Ca2+]c), which is responsible for the damage to and eventual death of pancreatic acinar cells. Vacuolization is an important indicator of pancreatic acinar cell damage. Furthermore, activation of trypsinogen occurs in the endocytic vacuoles; therefore the vacuoles can be considered as ‘initiating’ organelles in the development of the cell injury. In the present study, we investigated the relationship between the formation of endocytic vacuoles and Ca2+ influx developed in response to the inducers of acute pancreatitis [bile acid taurolithocholic acid 3-sulfate (TLC-S) and supramaximal concentration of cholecystokinin-8 (CCK)]. We found that the inhibitor of STIM (stromal interaction molecule)/Orai channels, GSK-7975A, effectively suppressed both the Ca2+ influx (stimulated by inducers of pancreatitis) and the formation of endocytic vacuoles. Cell death induced by TLC-S or CCK was also inhibited by GSK-7975A. We documented the formation of endocytic vacuoles in response to store-operated Ca2+ entry (SOCE) induced by thapsigargin [TG; inhibitor of sarcoplasmic/endoplasmic reticulum (ER) Ca2+ pumps] and observed strong inhibition of TG-induced vacuole formation by GSK-7975A. Finally, we found that structurally-unrelated inhibitors of calpain suppress formation of endocytic vacuoles, suggesting that this Ca2+-dependent protease is a mediator between Ca2+ elevation and endocytic vacuole formation. PMID:25370603

  1. The cell biology of the endocytic system from an evolutionary perspective.

    Science.gov (United States)

    Wideman, Jeremy G; Leung, Ka Fai; Field, Mark C; Dacks, Joel B

    2014-04-01

    Evolutionary cell biology can afford an interdisciplinary comparative view that gives insights into both the functioning of modern cells and the origins of cellular systems, including the endocytic organelles. Here, we explore several recent evolutionary cell biology studies, highlighting investigations into the origin and diversity of endocytic systems in eukaryotes. Beginning with a brief overview of the eukaryote tree of life, we show how understanding the endocytic machinery in a select, but diverse, array of organisms provides insights into endocytic system origins and predicts the likely configuration in the last eukaryotic common ancestor (LECA). Next, we consider three examples in which a comparative approach yielded insight into the function of modern cellular systems. First, using ESCRT-0 as an example, we show how comparative cell biology can discover both lineage-specific novelties (ESCRT-0) as well as previously ignored ancient proteins (Tom1), likely of both evolutionary and functional importance. Second, we highlight the power of comparative cell biology for discovery of previously ignored but potentially ancient complexes (AP5). Finally, using examples from ciliates and trypanosomes, we show that not all organisms possess canonical endocytic pathways, but instead likely evolved lineage-specific mechanisms. Drawing from these case studies, we conclude that a comparative approach is a powerful strategy for advancing knowledge about the general mechanisms and functions of endocytic systems.

  2. 49 CFR 236.828 - System, traffic control.

    Science.gov (United States)

    2010-10-01

    ... 49 Transportation 4 2010-10-01 2010-10-01 false System, traffic control. 236.828 Section 236.828..., MAINTENANCE, AND REPAIR OF SIGNAL AND TRAIN CONTROL SYSTEMS, DEVICES, AND APPLIANCES Definitions § 236.828 System, traffic control. A block signal system under which train movements are authorized by block...

  3. Probiotics promote endocytic allergen degradation in gut epithelial cells

    Energy Technology Data Exchange (ETDEWEB)

    Song, Chun-Hua [Department of Epidemiology and Biostatistics, College of Public Health, Zhengzhou University, Zhengzhou (China); Liu, Zhi-Qiang [Department of Gastroenterology, The Second Hospital, Zhengzhou University, Zhengzhou (China); Department of Pathology and Molecular Medicine, McMaster University, Hamilton, ON (Canada); Huang, Shelly [Department of Pathology and Molecular Medicine, McMaster University, Hamilton, ON (Canada); Zheng, Peng-Yuan, E-mail: medp7123@126.com [Department of Gastroenterology, The Second Hospital, Zhengzhou University, Zhengzhou (China); Yang, Ping-Chang, E-mail: yangp@mcmaster.ca [Department of Pathology and Molecular Medicine, McMaster University, Hamilton, ON (Canada)

    2012-09-14

    Highlights: Black-Right-Pointing-Pointer Knockdown of A20 compromised the epithelial barrier function. Black-Right-Pointing-Pointer The fusion of endosome/lysosome was disturbed in the A20-deficient HT-29 cells. Black-Right-Pointing-Pointer Antigens transported across A20-deficient HT-29 monolayers conserved antigenicity. Black-Right-Pointing-Pointer Probiotic proteins increased the expression of A20 in HT-29 cells. -- Abstract: Background and aims: Epithelial barrier dysfunction plays a critical role in the pathogenesis of allergic diseases; the mechanism is to be further understood. The ubiquitin E3 ligase A20 (A20) plays a role in the endocytic protein degradation in the cells. This study aims to elucidate the role of A20 in the maintenance of gut epithelial barrier function. Methods: Gut epithelial cell line, HT-29 cell, was cultured into monolayers to evaluate the barrier function in transwells. RNA interference was employed to knock down the A20 gene in HT-29 cells to test the role of A20 in the maintenance of epithelial barrier function. Probiotic derived proteins were extracted from the culture supernatants using to enhance the expression of A20 in HT-29 cells. Results: The results showed that the knockdown of A20 compromised the epithelial barrier function in HT-29 monolayers, mainly increased the intracellular permeability. The fusion of endosome/lysosome was disturbed in the A20-deficient HT-29 cells. Allergens collected from the transwell basal chambers of A20-deficient HT-29 monolayers still conserved functional antigenicity. Treating with probiotic derived proteins increased the expression of A20 in HT-29 cells and promote the barrier function. Conclusion: A20 plays an important role in the maintenance of epithelial barrier function as shown by HT-29 monolayer. Probiotic derived protein increases the expression of A20 and promote the HT-29 monolayer barrier function.

  4. Tanshinone IIA sodium sulfonate facilitates endocytic HMGB1 uptake.

    Science.gov (United States)

    Zhang, Yusong; Li, Wei; Zhu, Shu; Jundoria, Arvin; Li, Jianhua; Yang, Huan; Fan, Saijun; Wang, Ping; Tracey, Kevin J; Sama, Andrew E; Wang, Haichao

    2012-12-01

    Our seminal discovery of high mobility group box 1 (HMGB1) as a late mediator of lethal systemic inflammation has prompted a new field of investigation for the development of experimental therapeutics. We previously reported that a major Danshen ingredient, tanshinone IIA sodium sulfonate (TSN-SS), selectively inhibited endotoxin-induced HMGB1 release and conferred protection against lethal endotoxemia and sepsis. To investigate the underlying mechanisms by which TSN-SS effectively inhibits HMGB1 release, we examined whether TSN-SS stimulates HMGB1 uptake by macrophages and whether genetic depletion of HMGB1 receptors [e.g., toll-like receptors (TLR)2, TLR4, or the receptor for advanced glycation end product (RAGE)] or pharmacological inhibition of endocytosis impairs TSN-SS-facilitated HMGB1 cellular uptake. TSN-SS stimulated internalization of exogenous HMGB1 protein into macrophage cytoplasmic vesicles that subsequently co-localized with microtubule-associated protein light chain 3 (LC3)-positive punctate structures (likely amphisomes). Meanwhile, it time-dependently elevated cellular levels of internalized HMGB1, leading to elevated LC3-II production and aggregation. Although genetic depletion of TLR2, TLR4, and/or RAGE did not impair TSN-SS-mediated HMGB1 uptake, specific inhibitors of the clathrin- and caveolin-dependent endocytosis significantly impaired TSN-SS-mediated HMGB1 uptake. Co-treatment with a lysosomal inhibitor, bafilomycin A1, led to enhanced accumulation of endogenous LC3-II and internalized exogenous HMGB1 in TSN-SS/rHMGB1-treated macrophages. Taken together, these findings suggest that TSN-SS may facilitate HMGB1 endocytic uptake, and subsequently delivered it to LC3-positive vacuoles (possibly amphisomes) for degradation via a lysosome-dependent pathway. Copyright © 2012 Elsevier Inc. All rights reserved.

  5. Effects of photodynamic therapy on the endocytic pathway

    Science.gov (United States)

    Kessel, David; Price, Michael; Caruso, Joseph; Reiners, John

    2011-01-01

    In this report, we describe an effect of photodynamic therapy (PDT) on membrane trafficking in murine 1c1c7 hepatoma cells. A brief exposure of 1c1c7 cells to a 20 nM concentration of the phosphatidylinositol kinase class-3 antagonist wortmannin led to the rapid appearance of cytoplasmic vacuoles. Fluorescence monitoring of plasma membrane-associated 1-[4-(trimethylamino)-phenyl]-6-phenylhexa-1,3,5-triene (TDPH) over time demonstrated that the wortmannin-induced vacuoles were derived from endocytosed plasma membrane. Low-dose photodamage catalyzed by the lysosomal photosensitizer NPe6, prior to the addition of wortmannin, prevented formation of these vacuoles. NPe6 was found to suppress for several hours the normal trafficking of TDPH-labeled plasma membrane to the cytosol, and the formation of punctate TDPH-labeled cytoplasmic vesicles. The ability of NPe6-induced photodamage to suppress wortmannin-induced vacuolization occurred under conditions that did not disrupt lysosomes and were at or below the threshold of cytostatic/cytotoxic effects. Furthermore, the suppressive effects of NPe6-PDT were not prevented by inclusion of an agent that stabilized lysosomal membranes, or by E64d, an inhibitor of lysosomal cathepsin proteases. Mitochondrial photodamage was less effective at preventing wortmannin-induced vacuole formation and PDT directed against the ER had no effect. The role of photodamage to the endocytic pathway may be a hitherto unexplored effect on cells that selectively accumulate photosensitizing agents. These results indicate that photodamage directed against endosomes/lysosomes has effects independent of the release of lysosomal proteases. PMID:21125114

  6. Cystatin F is secreted, but artificial modification of its C-terminus can induce its endocytic targeting.

    Science.gov (United States)

    Cappello, Fanny; Gatti, Evelina; Camossetto, Voahirana; David, Alexandre; Lelouard, Hugues; Pierre, Philippe

    2004-07-15

    Cystatins constitute a superfamily of cysteine protease inhibitors. A member of the type II secreted cystatin family, cystatin F, has been identified through different gene array experiments to be specifically expressed in hematopoietic cells as well as to be associated with several malignant tumors, suggesting a role in immunity or cancer progression. Cystatin F specificity as a protease inhibitor is still elusive, and understanding the cellular traffic of this molecule is therefore a major step in its characterization. Although the mannosylation-6 phosphate of cystatin F has been suggested, no conclusive evidences of its endosomal targeting have been reported. Here we show using U937 cells that cystatin F is secreted as a disulfide bridge-linked dimer and is not associated with endosomes intracellularly. Interestingly, although cystatin F targeting to endosomes or lysosomes is not observed in U937, modification of its C-terminal end by the addition of several amino acids promotes its accumulation in the lysosomes of transfected HeLa cells. This observation suggests that cystatin F can be targeted to the endocytic pathway under specific conditions and its C-terminal domain might contribute to this event.

  7. TRAFFIC SIMULATION FOR MIXED TRAFFIC SYSTEMS

    African Journals Online (AJOL)

    EGETE

    2012-05-04

    2002). Description of a microscopic traffic model of an urban district and the analysis and problem solving traffic congestion based on actual data is its objective. There suggested models for a vehicular traffic flow based on partial ...

  8. Paxillin facilitates timely neurite initiation on soft-substrate environments by interacting with the endocytic machinery.

    Science.gov (United States)

    Chang, Ting-Ya; Chen, Chen; Lee, Min; Chang, Ya-Chu; Lu, Chi-Huan; Lu, Shao-Tzu; Wang, De-Yao; Wang, Aijun; Guo, Chin-Lin; Cheng, Pei-Lin

    2017-12-22

    Neurite initiation is the first step in neuronal development and occurs spontaneously in soft tissue environments. Although the mechanisms regulating the morphology of migratory cells on rigid substrates in cell culture are widely known, how soft environments modulate neurite initiation remains elusive. Using hydrogel cultures, pharmacologic inhibition, and genetic approaches, we reveal that paxillin-linked endocytosis and adhesion are components of a bistable switch controlling neurite initiation in a substrate modulus-dependent manner. On soft substrates, most paxillin binds to endocytic factors and facilitates vesicle invagination, elevating neuritogenic Rac1 activity and expression of genes encoding the endocytic machinery. By contrast, on rigid substrates, cells develop extensive adhesions, increase RhoA activity and sequester paxillin from the endocytic machinery, thereby delaying neurite initiation. Our results highlight paxillin as a core molecule in substrate modulus-controlled morphogenesis and define a mechanism whereby neuronal cells respond to environments exhibiting varying mechanical properties.

  9. Functional Links Between Aβ Toxicity, Endocytic Trafficking and Alzheimer’s Disease Risk Factors in Yeast

    Science.gov (United States)

    Treusch, Sebastian; Hamamichi, Shusei; Goodman, Jessica L.; Matlack, Kent E.S.; Chung, Chee Yeun; Baru, Valeriya; Shulman, Joshua M.; Parrado, Antonio; Bevis, Brooke J.; Valastyan, Julie S.; Han, Haesun; Lindhagen-Persson, Malin; Reiman, Eric M.; Evans, Denis A.; Bennett, David A.; Olofsson, Anders; DeJager, Philip L.; Tanzi, Rudolph E.; Caldwell, Kim A.; Caldwell, Guy A.; Lindquist, Susan

    2012-01-01

    Aβ (amyloid beta peptide) is an important contributor to Alzheimer’s disease (AD). We modeled Aβ toxicity in yeast by directing the peptide to the secretory pathway. A genome-wide screen for toxicity modifiers identified the yeast homolog of phosphatidylinositol binding clathrin assembly protein (PICALM) and other endocytic factors connected to AD whose relationship to Aβ was previously unknown. The factors identified in yeast modified Aβ toxicity in glutamatergic neurons of Caenorhabditis elegans and in primary rat cortical neurons. In yeast, Aβ impaired the endocytic trafficking of a plasma membrane receptor, which was ameliorated by endocytic pathway factors identified in the yeast screen. These links between Aβ, endocytosis, and human AD risk factors can be ascertained using yeast as a model system. PMID:22033521

  10. Functional links between Aβ toxicity, endocytic trafficking, and Alzheimer's disease risk factors in yeast.

    Science.gov (United States)

    Treusch, Sebastian; Hamamichi, Shusei; Goodman, Jessica L; Matlack, Kent E S; Chung, Chee Yeun; Baru, Valeriya; Shulman, Joshua M; Parrado, Antonio; Bevis, Brooke J; Valastyan, Julie S; Han, Haesun; Lindhagen-Persson, Malin; Reiman, Eric M; Evans, Denis A; Bennett, David A; Olofsson, Anders; DeJager, Philip L; Tanzi, Rudolph E; Caldwell, Kim A; Caldwell, Guy A; Lindquist, Susan

    2011-12-02

    Aβ (beta-amyloid peptide) is an important contributor to Alzheimer's disease (AD). We modeled Aβ toxicity in yeast by directing the peptide to the secretory pathway. A genome-wide screen for toxicity modifiers identified the yeast homolog of phosphatidylinositol binding clathrin assembly protein (PICALM) and other endocytic factors connected to AD whose relationship to Aβ was previously unknown. The factors identified in yeast modified Aβ toxicity in glutamatergic neurons of Caenorhabditis elegans and in primary rat cortical neurons. In yeast, Aβ impaired the endocytic trafficking of a plasma membrane receptor, which was ameliorated by endocytic pathway factors identified in the yeast screen. Thus, links between Aβ, endocytosis, and human AD risk factors can be ascertained with yeast as a model system.

  11. Sortin2 enhances endocytic trafficking towards the vacuole in Saccharomyces cerevisiae

    Directory of Open Access Journals (Sweden)

    Beatriz Vásquez-Soto

    2015-01-01

    Full Text Available BACKGROUND: A highly regulated trafficking of cargo vesicles in eukaryotes performs protein delivery to a variety of cellular compartments of endomembrane system. The two main routes, the secretory and the endocytic pathways have pivotal functions in uni- and multi-cellular organisms. Protein delivery and targeting includes cargo recognition, vesicle formation and fusion. Developing new tools to modulate protein trafficking allows better understanding the endomembrane system mechanisms and their regulation. The compound Sortin2 has been described as a protein trafficking modulator affecting targeting of the vacuolar protein carboxypeptidase Y (CPY, triggering its secretion in Saccharomyces cerevisiae. RESULTS: A reverse chemical-genetics approach was used to identify key proteins for Sortin2 bioactivity. A genome-wide Sortin2 resistance screen revealed six yeast deletion mutants that do not secrete CPY when grown at Sortin2 condition where the parental strain does: met18, sla1, clc1, dfg10, dpl1 and yjl175w. Integrating mutant phenotype and gene ontology annotation of the corresponding genes and their interactome pointed towards a high representation of genes involved in the endocytic process. In wild type yeast endocytosis towards the vacuole was faster in presence of Sortin2, which further validates the data of the genome-wide screen. This effect of Sortin2 depends on structural features of the molecule, suggesting compound specificity. Sortin2 did not affect endocytic trafficking in Sortin2-resistant mutants, strongly suggesting that the Sortin2 effects on the secretory and endocytic pathways are linked. CONCLUSIONS: Overall, the results reveal that Sortin2 enhances the endocytic transport pathway in Saccharomyces cerevisiae. This cellular effect is most likely at the level where secretory and endocytic pathways are merged. Them Sortin2 specificity over the endomembrane system places it as a powerful biological modulator for cell biology.

  12. Endocytic Pathways Involved in Filovirus Entry: Advances, Implications and Future Directions

    Directory of Open Access Journals (Sweden)

    Suchita Bhattacharyya

    2012-12-01

    Full Text Available Detailed knowledge of the host-virus interactions that accompany filovirus entry into cells is expected to identify determinants of viral virulence and host range, and to yield targets for the development of antiviral therapeutics. While it is generally agreed that filovirus entry into the host cytoplasm requires viral internalization into acidic endosomal compartments and proteolytic cleavage of the envelope glycoprotein by endo/lysosomal cysteine proteases, our understanding of the specific endocytic pathways co-opted by filoviruses remains limited. This review addresses the current knowledge on cellular endocytic pathways implicated in filovirus entry, highlights the consensus as well as controversies, and discusses important remaining questions.

  13. The LXR-IDOL axis defines a clathrin-, caveolae-, and dynamin-independent endocytic route for LDLR internalization and lysosomal degradation.

    Science.gov (United States)

    Sorrentino, Vincenzo; Nelson, Jessica K; Maspero, Elena; Marques, André R A; Scheer, Lilith; Polo, Simona; Zelcer, Noam

    2013-08-01

    Low density lipoprotein (LDL) cholesterol is taken up into cells via clathrin-mediated endocytosis of the LDL receptor (LDLR). Following dissociation of the LDLR-LDL complex, LDL is directed to lysosomes whereas the LDLR recycles to the plasma membrane. Activation of the sterol-sensing nuclear receptors liver X receptors (LXRs) enhances degradation of the LDLR. This depends on the LXR target gene inducible degrader of the LDLR (IDOL), an E3-ubiquitin ligase that promotes ubiquitylation and lysosomal degradation of the LDLR. How ubiquitylation of the LDLR by IDOL controls its endocytic trafficking is currently unknown. Using genetic- and pharmacological-based approaches coupled to functional assessment of LDL uptake, we show that the LXR-IDOL axis targets a LDLR pool present in lipid rafts. IDOL-dependent internalization of the LDLR is independent of clathrin, caveolin, macroautophagy, and dynamin. Rather, it depends on the endocytic protein epsin. Consistent with LDLR ubiquitylation acting as a sorting signal, degradation of the receptor can be blocked by perturbing the endosomal sorting complex required for transport (ESCRT) or by USP8, a deubiquitylase implicated in sorting ubiquitylated cargo to multivesicular bodies. In summary, we provide evidence for the existence of an LXR-IDOL-mediated internalization pathway for the LDLR that is distinct from that used for lipoprotein uptake.

  14. The LXR-IDOL axis defines a clathrin-, caveolae-, and dynamin-independent endocytic route for LDLR internalization and lysosomal degradation[S

    Science.gov (United States)

    Sorrentino, Vincenzo; Nelson, Jessica K.; Maspero, Elena; Marques, André R. A.; Scheer, Lilith; Polo, Simona; Zelcer, Noam

    2013-01-01

    Low density lipoprotein (LDL) cholesterol is taken up into cells via clathrin-mediated endocytosis of the LDL receptor (LDLR). Following dissociation of the LDLR-LDL complex, LDL is directed to lysosomes whereas the LDLR recycles to the plasma membrane. Activation of the sterol-sensing nuclear receptors liver X receptors (LXRs) enhances degradation of the LDLR. This depends on the LXR target gene inducible degrader of the LDLR (IDOL), an E3-ubiquitin ligase that promotes ubiquitylation and lysosomal degradation of the LDLR. How ubiquitylation of the LDLR by IDOL controls its endocytic trafficking is currently unknown. Using genetic- and pharmacological-based approaches coupled to functional assessment of LDL uptake, we show that the LXR-IDOL axis targets a LDLR pool present in lipid rafts. IDOL-dependent internalization of the LDLR is independent of clathrin, caveolin, macroautophagy, and dynamin. Rather, it depends on the endocytic protein epsin. Consistent with LDLR ubiquitylation acting as a sorting signal, degradation of the receptor can be blocked by perturbing the endosomal sorting complex required for transport (ESCRT) or by USP8, a deubiquitylase implicated in sorting ubiquitylated cargo to multivesicular bodies. In summary, we provide evidence for the existence of an LXR-IDOL-mediated internalization pathway for the LDLR that is distinct from that used for lipoprotein uptake. PMID:23733886

  15. Megalin and cubilin are endocytic receptors involved in renal clearance of hemoglobin

    DEFF Research Database (Denmark)

    Gburek, Jakub; Verroust, Pierre J; Willnow, Thomas E

    2002-01-01

    The kidney is the main site of hemoglobin clearance and degradation in conditions of severe hemolysis. Herein it is reported that megalin and cubilin, two epithelial endocytic receptors, mediate the uptake of hemoglobin in renal proximal tubules. Both receptors were purified by use of hemoglobin...

  16. Gliadin peptide P31-43 localises to endocytic vesicles and interferes with their maturation.

    Directory of Open Access Journals (Sweden)

    Maria Vittoria Barone

    Full Text Available BACKGROUND: Celiac Disease (CD is both a frequent disease (1:100 and an interesting model of a disease induced by food. It consists in an immunogenic reaction to wheat gluten and glutenins that has been found to arise in a specific genetic background; however, this reaction is still only partially understood. Activation of innate immunity by gliadin peptides is an important component of the early events of the disease. In particular the so-called "toxic" A-gliadin peptide P31-43 induces several pleiotropic effects including Epidermal Growth Factor Receptor (EGFR-dependent actin remodelling and proliferation in cultured cell lines and in enterocytes from CD patients. These effects are mediated by delayed EGFR degradation and prolonged EGFR activation in endocytic vesicles. In the present study we investigated the effects of gliadin peptides on the trafficking and maturation of endocytic vesicles. METHODS/PRINCIPAL FINDINGS: Both P31-43 and the control P57-68 peptide labelled with fluorochromes were found to enter CaCo-2 cells and interact with the endocytic compartment in pulse and chase, time-lapse, experiments. P31-43 was localised to vesicles carrying early endocytic markers at time points when P57-68-carrying vesicles mature into late endosomes. In time-lapse experiments the trafficking of P31-43-labelled vesicles was delayed, regardless of the cargo they were carrying. Furthermore in celiac enterocytes, from cultured duodenal biopsies, P31-43 trafficking is delayed in early endocytic vesicles. A sequence similarity search revealed that P31-43 is strikingly similar to Hrs, a key molecule regulating endocytic maturation. A-gliadin peptide P31-43 interfered with Hrs correct localisation to early endosomes as revealed by western blot and immunofluorescence microscopy. CONCLUSIONS: P31-43 and P57-68 enter cells by endocytosis. Only P31-43 localises at the endocytic membranes and delays vesicle trafficking by interfering with Hrs

  17. TrafficTurk evaluation.

    Science.gov (United States)

    2014-04-01

    This report summarizes a project undertaken by the University of Illinois on behalf of the Illinois Department of : Transportation to evaluate a smartphone application called TrafficTurk for traffic safety and traffic monitoring : applications. Traff...

  18. Population Blocks.

    Science.gov (United States)

    Smith, Martin H.

    1992-01-01

    Describes an educational game called "Population Blocks" that is designed to illustrate the concept of exponential growth of the human population and some potential effects of overpopulation. The game material consists of wooden blocks; 18 blocks are painted green (representing land), 7 are painted blue (representing water); and the remaining…

  19. Differential actions of the endocytic collagen receptor uPARAP/Endo180 and the collagenase MMP-2 in bone homeostasis

    DEFF Research Database (Denmark)

    Madsen, Daniel H; Jürgensen, Henrik J; Ingvarsen, Signe

    2013-01-01

    A well-coordinated remodeling of uncalcified collagen matrices is a pre-requisite for bone development and homeostasis. Collagen turnover proceeds through different pathways, either involving extracellular reactions exclusively, or being dependent on endocytic processes. Extracellular collagen...

  20. Traffic planning for non-homogeneous traffic

    Indian Academy of Sciences (India)

    2.3c Data summary The summarization of the density data based on videotape obser- vations is in table 1 which shows average, 30-second, sampled densities. Using the non- homogeneous traffic continuity equation of (2), the resultant traffic concentrations appear in table 2. Comparing the traffic concentrations in table 1 to ...

  1. Salmonella Disrupts Host Endocytic Trafficking by SopD2-Mediated Inhibition of Rab7

    Directory of Open Access Journals (Sweden)

    Vanessa M. D’Costa

    2015-09-01

    Full Text Available Intracellular bacterial pathogens of a diverse nature share the ability to evade host immunity by impairing trafficking of endocytic cargo to lysosomes for degradation, a process that is poorly understood. Here, we show that the Salmonella enterica type 3 secreted effector SopD2 mediates this process by binding the host regulatory GTPase Rab7 and inhibiting its nucleotide exchange. Consequently, this limits Rab7 interaction with its dynein- and kinesin-binding effectors RILP and FYCO1 and thereby disrupts host-driven regulation of microtubule motors. Our study identifies a bacterial effector capable of directly binding and thereby modulating Rab7 activity and a mechanism of endocytic trafficking disruption that may provide insight into the pathogenesis of other bacteria. Additionally, we provide a powerful tool for the study of Rab7 function, and a potential therapeutic target.

  2. Residues in the Hendra Virus Fusion Protein Transmembrane Domain Are Critical for Endocytic Recycling

    OpenAIRE

    Popa, Andreea; Carter, James R.; Smith, Stacy E.; Hellman, Lance; Fried, Michael G.; Dutch, Rebecca Ellis

    2012-01-01

    Hendra virus is a highly pathogenic paramyxovirus classified as a biosafety level four agent. The fusion (F) protein of Hendra virus is critical for promoting viral entry and cell-to-cell fusion. To be fusogenically active, Hendra virus F must undergo endocytic recycling and cleavage by the endosomal/lysosomal protease cathepsin L, but the route of Hendra virus F following internalization and the recycling signals involved are poorly understood. We examined the intracellular distribution of H...

  3. A Comparative Study on the Alterations of Endocytic Pathways in Multiple Lysosomal Storage Disorders.

    Science.gov (United States)

    Rappaport, Jeff; Manthe, Rachel L; Solomon, Melani; Garnacho, Carmen; Muro, Silvia

    2016-02-01

    Many cellular activities and pharmaceutical interventions involve endocytosis and delivery to lysosomes for processing. Hence, lysosomal processing defects can cause cell and tissue damage, as in lysosomal storage diseases (LSDs) characterized by lysosomal accumulation of undegraded materials. This storage causes endocytic and trafficking alterations, which exacerbate disease and hinder treatment. However, there have been no systematic studies comparing different endocytic routes in LSDs. Here, we used genetic and pharmacological models of four LSDs (type A Niemann-Pick, type C Niemann-Pick, Fabry, and Gaucher diseases) and evaluated the pinocytic and receptor-mediated activity of the clathrin-, caveolae-, and macropinocytic routes. Bulk pinocytosis was diminished in all diseases, suggesting a generic endocytic alteration linked to lysosomal storage. Fluid-phase (dextran) and ligand (transferrin) uptake via the clathrin route were lower for all LSDs. Fluid-phase and ligand (cholera toxin B) uptake via the caveolar route were both affected but less acutely in Fabry or Gaucher diseases. Epidermal growth factor-induced macropinocytosis was altered in Niemann-Pick cells but not other LSDs. Intracellular trafficking of ligands was also distorted in LSD versus wild-type cells. The extent of these endocytic alterations paralleled the level of cholesterol storage in disease cell lines. Confirming this, pharmacological induction of cholesterol storage in wild-type cells disrupted endocytosis, and model therapeutics restored uptake in proportion to their efficacy in attenuating storage. This suggests a proportional and reversible relationship between endocytosis and lipid (cholesterol) storage. By analogy, the accumulation of biological material in other diseases, or foreign material from drugs or their carriers, may cause similar deficits, warranting further investigation.

  4. Legionella Effector AnkX Disrupts Host Cell Endocytic Recycling in a Phosphocholination-Dependent Manner

    Directory of Open Access Journals (Sweden)

    Samual C. Allgood

    2017-09-01

    Full Text Available The facultative intracellular bacterium Legionella pneumophila proliferates within amoebae and human alveolar macrophages, and it is the causative agent of Legionnaires' disease, a life-threatening pneumonia. Within host cells, L. pneumophila establishes a replicative haven by delivering numerous effector proteins into the host cytosol, many of which target membrane trafficking by manipulating the function of Rab GTPases. The Legionella effector AnkX is a phosphocholine transferase that covalently modifies host Rab1 and Rab35. However, a detailed understanding of the biological consequence of Rab GTPase phosphocholination remains elusive. Here, we broaden the understanding of AnkX function by presenting three lines of evidence that it interferes with host endocytic recycling. First, using immunogold transmission electron microscopy, we determined that GFP-tagged AnkX ectopically produced in mammalian cells localizes at the plasma membrane and tubular membrane compartments, sites consistent with targeting the endocytic recycling pathway. Furthermore, the C-terminal region of AnkX was responsible for association with the plasma membrane, and we determined that this region was also able to bind the phosphoinositide lipids PI(3P and PI(4P in vitro. Second, we observed that mCherry-AnkX co-localized with Rab35, a regulator of recycling endocytosis and with major histocompatibility class I protein (MHC-I, a key immunoregulatory protein whose recycling from and back to the plasma membrane is Rab35-dependent. Third, we report that during infection of macrophages, AnkX is responsible for the disruption of endocytic recycling of transferrin, and AnkX's phosphocholination activity is critical for this function. These results support the hypothesis that AnkX targets endocytic recycling during host cell infection. Finally, we have demonstrated that the phosphocholination activity of AnkX is also critical for inhibiting fusion of the Legionella

  5. 7 CFR 502.12 - Vehicular and pedestrian traffic.

    Science.gov (United States)

    2010-01-01

    ... and directions of the security staff and all posted traffic signs; (b) The blocking of entrances..., DEPARTMENT OF AGRICULTURE CONDUCT ON BELTSVILLE AGRICULTURE RESEARCH CENTER PROPERTY, BELTSVILLE, MARYLAND.... (d) The operation of unlicensed gasoline powered vehicles is prohibited. ...

  6. The minute virus of mice exploits different endocytic pathways for cellular uptake

    Energy Technology Data Exchange (ETDEWEB)

    Garcin, Pierre O.; Panté, Nelly, E-mail: pante@zoology.ubc.ca

    2015-08-15

    The minute virus of mice, prototype strain (MVMp), is a non-enveloped, single-stranded DNA virus of the family Parvoviridae. Unlike other parvoviruses, the mechanism of cellular uptake of MVMp has not been studied in detail. We analyzed MVMp endocytosis in mouse LA9 fibroblasts and a tumor cell line derived from epithelial–mesenchymal transition through polyomavirus middle T antigen transformation in transgenic mice. By a combination of immunofluorescence and electron microscopy, we found that MVMp endocytosis occurs at the leading edge of migrating cells in proximity to focal adhesion sites. By using drug inhibitors of various endocytic pathways together with immunofluorescence microscopy and flow cytometry analysis, we discovered that MVMp can use a number of endocytic pathways, depending on the host cell type. At least three different mechanisms were identified: clathrin-, caveolin-, and clathrin-independent carrier-mediated endocytosis, with the latter occurring in transformed cells but not in LA9 fibroblasts. - Highlights: • MVMp uptake takes place at the leading edge of migrating cells. • MVMp exploits a variety of endocytic pathways. • MVMp could use clathrin- and caveolin-mediated endocytosis. • MVMp could also use clathrin-independent carriers for cellular uptake.

  7. The minute virus of mice exploits different endocytic pathways for cellular uptake

    International Nuclear Information System (INIS)

    Garcin, Pierre O.; Panté, Nelly

    2015-01-01

    The minute virus of mice, prototype strain (MVMp), is a non-enveloped, single-stranded DNA virus of the family Parvoviridae. Unlike other parvoviruses, the mechanism of cellular uptake of MVMp has not been studied in detail. We analyzed MVMp endocytosis in mouse LA9 fibroblasts and a tumor cell line derived from epithelial–mesenchymal transition through polyomavirus middle T antigen transformation in transgenic mice. By a combination of immunofluorescence and electron microscopy, we found that MVMp endocytosis occurs at the leading edge of migrating cells in proximity to focal adhesion sites. By using drug inhibitors of various endocytic pathways together with immunofluorescence microscopy and flow cytometry analysis, we discovered that MVMp can use a number of endocytic pathways, depending on the host cell type. At least three different mechanisms were identified: clathrin-, caveolin-, and clathrin-independent carrier-mediated endocytosis, with the latter occurring in transformed cells but not in LA9 fibroblasts. - Highlights: • MVMp uptake takes place at the leading edge of migrating cells. • MVMp exploits a variety of endocytic pathways. • MVMp could use clathrin- and caveolin-mediated endocytosis. • MVMp could also use clathrin-independent carriers for cellular uptake

  8. Endocytic Pathways Used by Andes Virus to Enter Primary Human Lung Endothelial Cells.

    Directory of Open Access Journals (Sweden)

    Cheng-Feng Chiang

    Full Text Available Andes virus (ANDV is the major cause of hantavirus pulmonary syndrome (HPS in South America. Despite a high fatality rate (up to 40%, no vaccines or antiviral therapies are approved to treat ANDV infection. To understand the role of endocytic pathways in ANDV infection, we used 3 complementary approaches to identify cellular factors required for ANDV entry into human lung microvascular endothelial cells. We screened an siRNA library targeting 140 genes involved in membrane trafficking, and identified 55 genes required for ANDV infection. These genes control the major endocytic pathways, endosomal transport, cell signaling, and cytoskeleton rearrangement. We then used infectious ANDV and retroviral pseudovirions to further characterize the possible involvement of 9 of these genes in the early steps of ANDV entry. In addition, we used markers of cellular endocytosis along with chemical inhibitors of known endocytic pathways to show that ANDV uses multiple routes of entry to infect target cells. These entry mechanisms are mainly clathrin-, dynamin-, and cholesterol-dependent, but can also occur via a clathrin-independent manner.

  9. The CD63-Syntenin-1 Complex Controls Post-Endocytic Trafficking of Oncogenic Human Papillomaviruses.

    Science.gov (United States)

    Gräßel, Linda; Fast, Laura Aline; Scheffer, Konstanze D; Boukhallouk, Fatima; Spoden, Gilles A; Tenzer, Stefan; Boller, Klaus; Bago, Ruzica; Rajesh, Sundaresan; Overduin, Michael; Berditchevski, Fedor; Florin, Luise

    2016-08-31

    Human papillomaviruses enter host cells via a clathrin-independent endocytic pathway involving tetraspanin proteins. However, post-endocytic trafficking required for virus capsid disassembly remains unclear. Here we demonstrate that the early trafficking pathway of internalised HPV particles involves tetraspanin CD63, syntenin-1 and ESCRT-associated adaptor protein ALIX. Following internalisation, viral particles are found in CD63-positive endosomes recruiting syntenin-1, a CD63-interacting adaptor protein. Electron microscopy and immunofluorescence experiments indicate that the CD63-syntenin-1 complex controls delivery of internalised viral particles to multivesicular endosomes. Accordingly, infectivity of high-risk HPV types 16, 18 and 31 as well as disassembly and post-uncoating processing of viral particles was markedly suppressed in CD63 or syntenin-1 depleted cells. Our analyses also present the syntenin-1 interacting protein ALIX as critical for HPV infection and CD63-syntenin-1-ALIX complex formation as a prerequisite for intracellular transport enabling viral capsid disassembly. Thus, our results identify the CD63-syntenin-1-ALIX complex as a key regulatory component in post-endocytic HPV trafficking.

  10. Determining Cell-surface Expression and Endocytic Rate of Proteins in Primary Astrocyte Cultures Using Biotinylation.

    Science.gov (United States)

    Tham, Daniel Kai Long; Moukhles, Hakima

    2017-07-03

    Cell-surface proteins mediate a wide array of functions. In many cases, their activity is regulated by endocytic processes that modulate their levels at the plasma membrane. Here, we present detailed protocols for 2 methods that facilitate the study of such processes, both of which are based on the principle of the biotinylation of cell-surface proteins. The first is designed to allow for the semi-quantitative determination of the relative levels of a particular protein at the cell-surface. In it, the lysine residues of the plasma membrane proteins of cells are first labeled with a biotin moiety. Once the cells are lysed, these proteins may then be specifically precipitated via the use of agarose-immobilized streptavidin by exploiting the natural affinity of the latter for biotin. The proteins isolated in such a manner may then be analyzed via a standard western blotting approach. The second method provides a means of determining the endocytic rate of a particular cell-surface target over a period of time. Cell-surface proteins are first modified with a biotin derivative containing a cleavable disulfide bond. The cells are then shifted back to normal culture conditions, which causes the endocytic uptake of a proportion of biotinylated proteins. Next, the disulfide bonds of non-internalized biotin groups are reduced using the membrane-impermeable reducing agent glutathione. Via this approach, endocytosed proteins may thus be isolated and quantified with a high degree of specificity.

  11. Vehicle-class Specific Control of Freeway Traffic

    NARCIS (Netherlands)

    Schreiter, T.

    2013-01-01

    The increase of mobility of the past decades has led to substantial congestion on the freeways. Traffic jams emerge both on a daily basis at the same location, as well as during accidents when a part of the freeways is temporarily blocked. In those cases, traffic management centers intervene into

  12. Queueing and traffic

    NARCIS (Netherlands)

    Baër, Niek

    2015-01-01

    Traffic jams are everywhere, some are caused by constructions or accidents but a large portion occurs naturally. These "natural" traffic jams are a result of variable driving speeds combined with a high number of vehicles. To prevent these traffic jams, we must understand traffic in general, and to

  13. Jamitons: Phantom Traffic Jams

    Science.gov (United States)

    Kowszun, Jorj

    2013-01-01

    Traffic on motorways can slow down for no apparent reason. Sudden changes in speed by one or two drivers can create a chain reaction that causes a traffic jam for the vehicles that are following. This kind of phantom traffic jam is called a "jamiton" and the article discusses some of the ways in which traffic engineers produce…

  14. ΔF508 CFTR surface stability is regulated by DAB2 and CHIP-mediated ubiquitination in post-endocytic compartments.

    Directory of Open Access Journals (Sweden)

    Lianwu Fu

    Full Text Available The ΔF508 mutant form of the cystic fibrosis transmembrane conductance regulator (ΔF508 CFTR that is normally degraded by the ER-associated degradative pathway can be rescued to the cell surface through low-temperature (27°C culture or small molecular corrector treatment. However, it is unstable on the cell surface, and rapidly internalized and targeted to the lysosomal compartment for degradation. To understand the mechanism of this rapid turnover, we examined the role of two adaptor complexes (AP-2 and Dab2 and three E3 ubiquitin ligases (c-Cbl, CHIP, and Nedd4-2 on low-temperature rescued ΔF508 CFTR endocytosis and degradation in human airway epithelial cells. Our results demonstrate that siRNA depletion of either AP-2 or Dab2 inhibits ΔF508 CFTR endocytosis by 69% and 83%, respectively. AP-2 or Dab2 depletion also increases the rescued protein half-life of ΔF508 CFTR by ~18% and ~91%, respectively. In contrast, the depletion of each of the E3 ligases had no effect on ΔF508 CFTR endocytosis, whereas CHIP depletion significantly increased the surface half-life of ΔF508 CFTR. To determine where and when the ubiquitination occurs during ΔF508 CFTR turnover, we monitored the ubiquitination of rescued ΔF508 CFTR during the time course of CFTR endocytosis. Our results indicate that ubiquitination of the surface pool of ΔF508 CFTR begins to increase 15 min after internalization, suggesting that CFTR is ubiquitinated in a post-endocytic compartment. This post-endocytic ubiquination of ΔF508 CFTR could be blocked by either inhibiting endocytosis, by siRNA knockdown of CHIP, or by treating cells with the CFTR corrector, VX-809. Our results indicate that the post-endocytic ubiquitination of CFTR by CHIP is a critical step in the peripheral quality control of cell surface ΔF508 CFTR.

  15. A Coincidence Detection Mechanism Controls PX-BAR Domain-Mediated Endocytic Membrane Remodeling via an Allosteric Structural Switch.

    Science.gov (United States)

    Lo, Wen-Ting; Vujičić Žagar, Andreja; Gerth, Fabian; Lehmann, Martin; Puchkov, Dymtro; Krylova, Oxana; Freund, Christian; Scapozza, Leonardo; Vadas, Oscar; Haucke, Volker

    2017-11-20

    Clathrin-mediated endocytosis occurs by bending and remodeling of the membrane underneath the coat. Bin-amphiphysin-rvs (BAR) domain proteins are crucial for endocytic membrane remodeling, but how their activity is spatiotemporally controlled is largely unknown. We demonstrate that the membrane remodeling activity of sorting nexin 9 (SNX9), a late-acting endocytic PX-BAR domain protein required for constriction of U-shaped endocytic intermediates, is controlled by an allosteric structural switch involving coincident detection of the clathrin adaptor AP2 and phosphatidylinositol-3,4-bisphosphate (PI(3,4)P 2 ) at endocytic sites. Structural, biochemical, and cell biological data show that SNX9 is autoinhibited in solution. Binding to PI(3,4)P 2 via its PX-BAR domain, and concomitant association with AP2 via sequences in the linker region, releases SNX9 autoinhibitory contacts to enable membrane constriction. Our results reveal a mechanism for restricting the latent membrane remodeling activity of BAR domain proteins to allow spatiotemporal coupling of membrane constriction to the progression of the endocytic pathway. Copyright © 2017 Elsevier Inc. All rights reserved.

  16. Traffic Sign Recognition System based on Cambridge Correlator Image Comparator

    Directory of Open Access Journals (Sweden)

    J. Turan

    2012-06-01

    Full Text Available Paper presents basic information about application of Optical Correlator (OC, specifically Cambridge Correlator, in system to recognize of traffic sign. Traffic Sign Recognition System consists of three main blocks, Preprocessing, Optical Correlator and Traffic Sign Identification. The Region of Interest (ROI is defined and chosen in preprocessing block and then goes to Optical Correlator, where is compared with database of Traffic Sign. Output of Optical Correlation is correlation plane, which consist of highly localized intensities, know as correlation peaks. The intensity of spots provides a measure of similarity and position of spots, how images (traffic signs are relatively aligned in the input scene. Several experiments have been done with proposed system and results and conclusion are discussed.

  17. Laminin and fibronectin treatment leads to generation of dendritic cells with superior endocytic capacity.

    Directory of Open Access Journals (Sweden)

    Samuel García-Nieto

    2010-04-01

    Full Text Available Sampling the microenvironment at sites of microbial exposure by dendritic cells (DC and their subsequent interaction with T cells in the paracortical area of lymph nodes are key events for initiating immune responses. Most of our knowledge of such events in human is based on in vitro studies performed in the absence of extracellular matrix (ECM proteins. ECM in basement membranes and interstitial spaces of different tissues, including lymphoid organs, plays an important role in controlling specific cellular functions such as migration, intracellular signalling and differentiation. The aim of this study was, therefore, to investigate the impact of two abundant ECM components, fibronectin and laminin, on the phenotypical and functional properties of DC and how that might influence DC induced T-cell differentiation.Human monocyte derived DC were treated with laminin and fibronectin for up to 48 hours and their morphology and phenotype was analyzed using scanning electron microscopy, flow cytometry and real time PCR. The endocytic ability of DC was determined using flow cytometry. Furthermore, co-culture of DC and T cells were established and T cell proliferation and cytokine profile was measured using H(3-thymidine incorporation and ELISA respectively. Finally, we assessed formation of DC-T cell conjugates using different cell trackers and flow cytometry. Our data show that in the presence of ECM, DC maintain a 'more immature' phenotype and express higher levels of key endocytic receptors, and as a result become significantly better endocytic cells, but still fully able to mature in response to stimulation as evidenced by their superior ability to induce antigen-specific T cell differentiation.These studies underline the importance of including ECM components in in vitro studies investigating DC biology and DC-T cell interaction. Within the context of antigen specific DC induced T cell proliferation, inclusion of ECM proteins could lead to

  18. Traffic management simulation development : summary.

    Science.gov (United States)

    2011-01-01

    Increasingly, Florida traffic is monitored electronically by components of the Intelligent Traffic System (ITS), which send data to regional traffic management centers and assist management of traffic flows and incident response using software called...

  19. National guidelines for traffic calming

    CSIR Research Space (South Africa)

    Schermers, G

    1998-03-01

    Full Text Available This document serves as a comprehensive national traffic calming guideline, compiled to assist traffic engineering practitioners and road authorities with the implementation of traffic calming. It outlines the different objectives of traffic calming...

  20. Traffic management simulation development.

    Science.gov (United States)

    2011-01-03

    Microscopic simulation can provide significant support to traffic management center (TMC) operations. However, traffic simulation applications require data that are expensive and time-consuming to collect. Data collected by TMCs can be used as a prim...

  1. Traffic signal timing manual

    Science.gov (United States)

    2008-06-01

    This report serves as a comprehensive guide to traffic signal timing and documents the tasks completed in association with its development. The focus of this document is on traffic signal control principles, practices, and procedures. It describes th...

  2. An intelligent traffic controller

    Science.gov (United States)

    1995-11-01

    Advances in computing sciences have not been applied to traffic control. This paper describes the development of an intelligent controller. A controller with advanced control logic can significantly improve traffic flows at intersections. In this vei...

  3. Vehiculization determines the endocytic internalization mechanism of Zn(II)-phthalocyanine.

    Science.gov (United States)

    Soriano, Jorge; Villanueva, Angeles; Stockert, Juan C; Cañete, Magdalena

    2013-01-01

    It is generally accepted that compounds of nanomolecular size penetrate into cells by different endocytic processes. The vehiculization strategy of a compound is a factor that could determine its uptake mechanism. Understanding the influence of the vehicle in the precise mechanism of drug penetration into cells makes possible to improve or modify the therapeutic effects. In this study, using human A-549 cells, we have characterized the possible internalization mechanism of the photosensitizer Zn(II)-phthalocyanine (ZnPc), either dissolved in dimethylformamide (ZnPc-DMF) or included in liposomes of dipalmitoyl-phosphatidyl-choline. Specific inhibitors involved in the main endocytic pathways were used. Co-incubation of cells with ZnPc-liposomes and dynasore (dinamin-mediated endocytosis inhibitor) resulted in a significant decrease of photodamage, whereas other inhibitors did not alter the photodynamic effect of ZnPc. On the contrary, cells treated with ZnPc-DMF in the presence of dynasore, genistein (caveolin-mediated endocytosis inhibitor) or cytochalasin D (macropinocytosis and caveolin-mediated endocytosis inhibitor) showed a significant decrease in ZnPc uptake and photodynamic damage. These results suggest that ZnPc-DMF penetrates into cells mainly by caveolin-mediated endocytosis, whereas ZnPc-liposomes are internalized into cells preferentially by clathrin-mediated endocytosis. We conclude that using different drug vehiculization systems, it is possible to modify the internalization mechanism of a therapeutic compound, which could be of great interest in clinical research.

  4. Role of the EHD Family of Endocytic Recycling Regulators for TCR Recycling and T Cell Function.

    Science.gov (United States)

    Iseka, Fany M; Goetz, Benjamin T; Mushtaq, Insha; An, Wei; Cypher, Luke R; Bielecki, Timothy A; Tom, Eric C; Arya, Priyanka; Bhattacharyya, Sohinee; Storck, Matthew D; Semerad, Craig L; Talmadge, James E; Mosley, R Lee; Band, Vimla; Band, Hamid

    2018-01-15

    T cells use the endocytic pathway for key cell biological functions, including receptor turnover and maintenance of the immunological synapse. Some of the established players include the Rab GTPases, the SNARE complex proteins, and others, which function together with EPS-15 homology domain-containing (EHD) proteins in non-T cell systems. To date, the role of the EHD protein family in T cell function remains unexplored. We generated conditional EHD1/3/4 knockout mice using CD4-Cre and crossed these with mice bearing a myelin oligodendrocyte glycoprotein-specific TCR transgene. We found that CD4 + T cells from these mice exhibited reduced Ag-driven proliferation and IL-2 secretion in vitro. In vivo, these mice exhibited reduced severity of experimental autoimmune encephalomyelitis. Further analyses showed that recycling of the TCR-CD3 complex was impaired, leading to increased lysosomal targeting and reduced surface levels on CD4 + T cells of EHD1/3/4 knockout mice. Our studies reveal a novel role of the EHD family of endocytic recycling regulatory proteins in TCR-mediated T cell functions. Copyright © 2018 by The American Association of Immunologists, Inc.

  5. Residues in the hendra virus fusion protein transmembrane domain are critical for endocytic recycling.

    Science.gov (United States)

    Popa, Andreea; Carter, James R; Smith, Stacy E; Hellman, Lance; Fried, Michael G; Dutch, Rebecca Ellis

    2012-03-01

    Hendra virus is a highly pathogenic paramyxovirus classified as a biosafety level four agent. The fusion (F) protein of Hendra virus is critical for promoting viral entry and cell-to-cell fusion. To be fusogenically active, Hendra virus F must undergo endocytic recycling and cleavage by the endosomal/lysosomal protease cathepsin L, but the route of Hendra virus F following internalization and the recycling signals involved are poorly understood. We examined the intracellular distribution of Hendra virus F following endocytosis and showed that it is primarily present in Rab5- and Rab4-positive endosomal compartments, suggesting that cathepsin L cleavage occurs in early endosomes. Hendra virus F transmembrane domain (TMD) residues S490 and Y498 were found to be important for correct Hendra virus F recycling, with the hydroxyl group of S490 and the aromatic ring of Y498 important for this process. In addition, changes in association of isolated Hendra virus F TMDs correlated with alterations to Hendra virus F recycling, suggesting that appropriate TMD interactions play an important role in endocytic trafficking.

  6. Stochastic Modeling of the Clathrin-dependent and -independent Endocytic Pathways

    Science.gov (United States)

    Deng, Hua; Dutta, Prashanta; Liu, Jin

    2017-11-01

    Endocytosis is one of the important processes that bioparticles use to enter the cells. During endocytosis the membrane-bound vesicles are formed by the invagination of plasma membrane as a result of interactions among many proteins and cytoskeletons. The clathrin-mediated endocytosis is one of the most significant form of endocytosis, where the dynamic assembly of clathrin-coated pits play a critical role. While herpes simplex virus-1 has recently shown to infect cell by a novel phagocytosis-like endocytic pathway where actin polymerization may facilitate the viral entry. In this work, we propose a stochastic model for both clathrin-dependent and -independent endocytic pathways based on Monte Carlo simulations. The important roles of clathrin coating and actin cytoskeleton as well as the impact of other biological parameters are studied. Our preliminary results indicate that there exist an intermediate particle size and ligand density that maximize the internalization efficiency. Below a critical size or surface ligand density, it is difficult for the entry of a single particle, which means clustering may needed for more efficient internalization. We also find that lower membrane bending rigidity may help promote the bioparticle entry. Research reported in this publication was supported by the National Institute of General Medical Sciences of the National Institutes of Health under Award Number R01GM122081.

  7. Lymphocytic choriomeningitis virus uses a novel endocytic pathway for infectious entry via late endosomes

    International Nuclear Information System (INIS)

    Quirin, Katharina; Eschli, Bruno; Scheu, Isabella; Poort, Linda; Kartenbeck, Juergen; Helenius, Ari

    2008-01-01

    The endocytic entry of lymphocytic choriomeningitis virus (LCMV) into host cells was compared to the entry of viruses known to exploit clathrin or caveolae/raft-dependent pathways. Pharmacological inhibitors, expression of pathway-specific dominant-negative constructs, and siRNA silencing of clathrin together with electron and light microscopy provided evidence that although a minority population followed a classical clathrin-mediated mechanism of entry, the majority of these enveloped RNA viruses used a novel endocytic route to late endosomes. The pathway was clathrin, dynamin-2, actin, Arf6, flotillin-1, caveolae, and lipid raft independent but required membrane cholesterol. Unaffected by perturbation of Rab5 or Rab7 and apparently without passing through Rab5/EEA1-positive early endosomes, the viruses reached late endosomes and underwent acid-induced penetration. This membrane trafficking route between the plasma membrane and late endosomes may function in the turnover of a select group of surface glycoproteins such as the dystroglycan complex, which serves as the receptor of LCMV

  8. Zebrafish kidney phagocytes utilize macropinocytosis and Ca+-dependent endocytic mechanisms.

    Directory of Open Access Journals (Sweden)

    Claudia Hohn

    Full Text Available BACKGROUND: The innate immune response constitutes the first line of defense against invading pathogens and consists of a variety of immune defense mechanisms including active endocytosis by macrophages and granulocytes. Endocytosis can be used as a reliable measure of selective and non-selective mechanisms of antigen uptake in the early phase of an immune response. Numerous assays have been developed to measure this response in a variety of mammalian and fish species. The small size of the zebrafish has prevented the large-scale collection of monocytes/macrophages and granulocytes for these endocytic assays. METHODOLOGY/PRINCIPAL FINDINGS: Pooled zebrafish kidney hematopoietic tissues were used as a source of phagocytic cells for flow-cytometry based endocytic assays. FITC-Dextran, Lucifer Yellow and FITC-Edwardsiella ictaluri were used to evaluate selective and non-selective mechanisms of uptake in zebrafish phagocytes. CONCLUSIONS/SIGNIFICANCE: Zebrafish kidney phagocytes characterized as monocytes/macrophages, neutrophils and lymphocytes utilize macropinocytosis and Ca(2+-dependant endocytosis mechanisms of antigen uptake. These cells do not appear to utilize a mannose receptor. Heat-killed Edwardsiella ictaluri induces cytoskeletal interactions for internalization in zebrafish kidney monocytes/macrophages and granulocytes. The proposed method is easy to implement and should prove especially useful in immunological, toxicological and epidemiological research.

  9. Traffic Light Options

    DEFF Research Database (Denmark)

    Jørgensen, Peter Løchte

    This paper introduces, prices, and analyzes traffic light options. The traffic light option is an innovative structured OTC derivative developed independently by several London-based investment banks to suit the needs of Danish life and pension (L&P) companies, which must comply with the traffic...... 2006, and supervisory authorities in many other European countries have implemented similar regulation. Traffic light options are therefore likely to attract the attention of a wider audience of pension fund managers in the future. Focusing on the valuation of the traffic light option we set up a Black...... light scenarios. These stress scenarios entail drops in interest rates as well as in stock prices, and traffic light options are thus designed to pay off and preserve sufficient capital when interest rates and stock prices fall simultaneously. Sweden's FSA implemented a traffic light system in January...

  10. A Marine Traffic Flow Model

    Directory of Open Access Journals (Sweden)

    Tsz Leung Yip

    2013-03-01

    Full Text Available A model is developed for studying marine traffic flow through classical traffic flow theories, which can provide us with a better understanding of the phenomenon of traffic flow of ships. On one hand, marine traffic has its special features and is fundamentally different from highway, air and pedestrian traffic. The existing traffic models cannot be simply extended to marine traffic without addressing marine traffic features. On the other hand, existing literature on marine traffic focuses on one ship or two ships but does not address the issues in marine traffic flow.

  11. Costs of traffic injuries

    DEFF Research Database (Denmark)

    Kruse, Marie

    2015-01-01

    OBJECTIVE: The aim of this study was to analyse the socioeconomic costs of traffic injuries in Denmark, notably the healthcare costs and the productivity costs related to traffic injuries, in a bottom-up, register-based perspective. METHOD: Traffic injury victims were identified using national...... emergency room data and police records. Victims were matched with five controls per case by means of propensity score, nearest-neighbour matching. In the cohort, consisting of the 52 526 individuals that experienced a traffic injury in 2000 and 262 630 matched controls, attributable healthcare costs were...... assessed using Danish national healthcare registers. Productivity costs were computed using duration analysis (Cox regression models). In a subanalysis, cost per severe traffic injury was computed for the 12 995 individuals that experienced a severe injury. RESULTS: The socioeconomic cost of a traffic...

  12. Traffic Signs Inventory System

    Directory of Open Access Journals (Sweden)

    J. Ružbarský

    2013-06-01

    Full Text Available The paper is focused on practical application of Cambridge Correlator. The goal is to propose a traffic signs inventory system by using excellent characteristics of correlator in the rapid optical correlation. The proposal of this inventory system includes obtaining of traffic signs to create the database either collecting the GPS coordinates. It is necessary to know the traffic signs position and also to document the entire surface route for later evaluation in offline mode.

  13. Megalin and cubilin are endocytic receptors involved in renal clearance of hemoglobin

    DEFF Research Database (Denmark)

    Gburek, Jakub; Verroust, Pierre J; Willnow, Thomas E

    2002-01-01

    -Sepharose affinity chromatography of solubilized renal brush-border membranes. Apparent dissociation constants of 1.7 microM for megalin and 4.1 microM for cubilin were determined by surface plasmon resonance analysis. The binding was calcium dependent in both cases. Uptake of fluorescence-labeled hemoglobin by BN......The kidney is the main site of hemoglobin clearance and degradation in conditions of severe hemolysis. Herein it is reported that megalin and cubilin, two epithelial endocytic receptors, mediate the uptake of hemoglobin in renal proximal tubules. Both receptors were purified by use of hemoglobin...... not affect the uptake. By use of immunohistochemistry, it was demonstrated that uptake of hemoglobin in proximal tubules of rat, mouse, and dog kidneys occurs under physiologic conditions. Studies on normal and megalin knockout mouse kidney sections showed that megalin is responsible for physiologic...

  14. Stem-cell-specific endocytic degradation defects lead to intestinal dysplasia in Drosophila

    Directory of Open Access Journals (Sweden)

    Péter Nagy

    2016-05-01

    Full Text Available UV radiation resistance-associated gene (UVRAG is a tumor suppressor involved in autophagy, endocytosis and DNA damage repair, but how its loss contributes to colorectal cancer is poorly understood. Here, we show that UVRAG deficiency in Drosophila intestinal stem cells leads to uncontrolled proliferation and impaired differentiation without preventing autophagy. As a result, affected animals suffer from gut dysfunction and short lifespan. Dysplasia upon loss of UVRAG is characterized by the accumulation of endocytosed ligands and sustained activation of STAT and JNK signaling, and attenuation of these pathways suppresses stem cell hyperproliferation. Importantly, the inhibition of early (dynamin-dependent or late (Rab7-dependent steps of endocytosis in intestinal stem cells also induces hyperproliferation and dysplasia. Our data raise the possibility that endocytic, but not autophagic, defects contribute to UVRAG-deficient colorectal cancer development in humans.

  15. Localization of HCMV UL33 and US27 in endocytic compartments and viral membranes

    DEFF Research Database (Denmark)

    Fraile-Ramos, Alberto; Pelchen-Matthews, Annegret; Kledal, Thomas N

    2002-01-01

    are located at the cell surface, although the majority of both proteins was seen in intracellular organelles located in the perinuclear region of the cell. The intracellular pools of UL33 and US27 showed overlap with markers for endocytic organelles. Antibody-feeding experiments indicated that cell surface US......27 undergoes endocytosis. By immunogold labeling of cryosections and electron microscopy, UL33 was seen to localize to multivesicular bodies (MVBs or multivesicular endosomes). Electron microscopy analysis of human cytomegalovirus-infected cells showed that most virus particles wrapped individually...... and undergoes constitutive endocytosis and recycling. Here we studied the cellular distributions and trafficking of two other human cytomegalovirus chemokine receptor-like proteins, UL33 and US27, in transfected and human cytomegalovirus-infected cells. Immunofluorescence staining indicated that UL33 and US27...

  16. Subversion of the Endocytic and Secretory Pathways by Bacterial Effector Proteins

    Directory of Open Access Journals (Sweden)

    Mary M. Weber

    2018-01-01

    Full Text Available Intracellular bacteria have developed numerous strategies to hijack host vesicular trafficking pathways to form their unique replicative niches. To promote intracellular replication, the bacteria must interact with host organelles and modulate host signaling pathways to acquire nutrients and membrane for the growing parasitophorous vacuole all while suppressing activation of the immune response. To facilitate host cell subversion, bacterial pathogens use specialized secretion systems to deliver bacterial virulence factors, termed effectors, into the host cell that mimic, agonize, and/or antagonize the function of host proteins. In this review we will discuss how bacterial effector proteins from Coxiella burnetii, Brucella abortus, Salmonella enterica serovar Typhimurium, Legionella pneumophila, Chlamydia trachomatis, and Orientia tsutsugamushi manipulate the endocytic and secretory pathways. Understanding how bacterial effector proteins manipulate host processes not only gives us keen insight into bacterial pathogenesis, but also enhances our understanding of how eukaryotic membrane trafficking is regulated.

  17. Subversion of the Endocytic and Secretory Pathways by Bacterial Effector Proteins.

    Science.gov (United States)

    Weber, Mary M; Faris, Robert

    2018-01-01

    Intracellular bacteria have developed numerous strategies to hijack host vesicular trafficking pathways to form their unique replicative niches. To promote intracellular replication, the bacteria must interact with host organelles and modulate host signaling pathways to acquire nutrients and membrane for the growing parasitophorous vacuole all while suppressing activation of the immune response. To facilitate host cell subversion, bacterial pathogens use specialized secretion systems to deliver bacterial virulence factors, termed effectors, into the host cell that mimic, agonize, and/or antagonize the function of host proteins. In this review we will discuss how bacterial effector proteins from Coxiella burnetii, Brucella abortus, Salmonella enterica serovar Typhimurium, Legionella pneumophila, Chlamydia trachomatis , and Orientia tsutsugamushi manipulate the endocytic and secretory pathways. Understanding how bacterial effector proteins manipulate host processes not only gives us keen insight into bacterial pathogenesis, but also enhances our understanding of how eukaryotic membrane trafficking is regulated.

  18. Localization of HCMV UL33 and US27 in endocytic compartments and viral membranes

    DEFF Research Database (Denmark)

    Fraile-Ramos, Alberto; Pelchen-Matthews, Annegret; Kledal, Thomas N

    2002-01-01

    The human cytomegalovirus genome encodes four putative seven transmembrane domain chemokine receptor-like proteins. Although important in viral pathogenesis, little is known about the properties or functions of these proteins. We previously reported that US28 is located in endocytic vesicles...... and undergoes constitutive endocytosis and recycling. Here we studied the cellular distributions and trafficking of two other human cytomegalovirus chemokine receptor-like proteins, UL33 and US27, in transfected and human cytomegalovirus-infected cells. Immunofluorescence staining indicated that UL33 and US27......27 undergoes endocytosis. By immunogold labeling of cryosections and electron microscopy, UL33 was seen to localize to multivesicular bodies (MVBs or multivesicular endosomes). Electron microscopy analysis of human cytomegalovirus-infected cells showed that most virus particles wrapped individually...

  19. Transient handover blocking probabilities in road covering cellular mobile networks

    NARCIS (Netherlands)

    Boucherie, Richardus J.; van der Wal, Jan

    2003-01-01

    This paper investigates handover and fresh call blocking probabilities for subscribers moving along a road in a traffic jam passing through consecutive cells of a wireless network. It is observed and theoretically motivated that the hand- over blocking probabilities show a sharp peak in the initial

  20. Transient handover blocking probabilities in road covering cellular mobile networks

    NARCIS (Netherlands)

    Boucherie, Richardus J.; van der Wal, J.

    2002-01-01

    This paper investigates handover and fresh call blocking probabilities for subscribers moving along a road in a traffic jam passing through consecutive cells of a wireless network. It is observed and theoretically motivated that the handover blocking probabilities show a sharp peak in the initial

  1. modified traffic s modified traffic signal phasing at traffic warden ...

    African Journals Online (AJOL)

    eobe

    obtained as being adequate for the critical approach. A 5 tained as being adequate for the critical approach. A 5 tained as being adequate for the critical approach. A 5-phase scheme is proposed with the fifth phase being an phase scheme is .... Other examples include traffic light signalized crossing with or without ...

  2. Altered TGF-β endocytic trafficking contributes to the increased signaling in Marfan syndrome.

    Science.gov (United States)

    Siegert, Anna-Maria; Serra-Peinado, Carla; Gutiérrez-Martínez, Enric; Rodríguez-Pascual, Fernando; Fabregat, Isabel; Egea, Gustavo

    2018-02-01

    The main cardiovascular alteration in Marfan syndrome (MFS) is the formation of aortic aneurysms in which augmented TGF-β signaling is reported. However, the primary role of TGF-β signaling as a molecular link between the genetic mutation of fibrillin-1 and disease onset is controversial. The compartmentalization of TGF-β endocytic trafficking has been shown to determine a signaling response in which clathrin-dependent internalization leads to TGF-β signal propagation, and caveolin-1 (CAV-1) associated internalization leads to signal abrogation. We here studied the contribution of endocytic trafficking compartmentalization to increased TGF-β signaling in vascular smooth muscle cells (VSMC) from MFS patients. We examined molecular components involved in clathrin- (SARA, SMAD2) and caveolin-1- (SMAD7, SMURF2) dependent endocytosis. Marfan VSMC showed higher recruitment of SARA and SMAD2 to membranes and their increased interaction with TGF-β receptor II, as well as higher colocalization of SARA with the early endosome marker EEA1. We assessed TGF-β internalization using a biotinylated ligand (b-TGF-β), which colocalized equally with either EEA1 or CAV-1 in VSMC from Marfan patients and controls. However, in Marfan cells, colocalization of b-TGF-β with SARA and EEA1 was increased and accompanied by decreased colocalization with CAV-1 at EEA1-positive endosomes. Moreover, Marfan VSMC showed higher transcriptional levels and membrane enrichment of RAB5. Our results indicate that increased RAB5-associated SARA localization to early endosomes facilitates its TGF-β receptor binding and phosphorylation of signaling mediator SMAD2 in Marfan VSMC. This is accompanied by a reduction of TGF-β sorting into multifunctional vesicles containing cargo from both internalization pathways. Copyright © 2017 Elsevier B.V. All rights reserved.

  3. INTELLIGENT TRAFFIC-SAFETY MIRROR BY USING WIRELESS SENSOR NETWORK

    Directory of Open Access Journals (Sweden)

    Peter Danišovič

    2014-03-01

    Full Text Available This article is focused on the problematic of traffic safety, dealing with the problem of car intersections with blocked view crossing by a special wireless sensor network (WSN proposed for the traffic monitoring, concretely for vehicle’s detection at places, where it is necessary. Some ultra-low-power TI products were developed due to this reason: microcontroller MSP430F2232, 868MHz RF transceiver CC1101 and LDO voltage regulator TPS7033. The WSN consist of four network nodes supplied with the special safety lightings which serve the function of intelligent traffic safety mirror.

  4. Image Pre-processing in Vertical Traffic Signs Detection System

    Directory of Open Access Journals (Sweden)

    Dávid Solus

    2015-06-01

    Full Text Available The aim of this paper is to present the first steps of the systems design that will be able to detect vertical traffic signs and to provide descriptions of the applied data processing methods. This system includes various functional blocks that are described in this paper. The basis of Vertical Traffic Signs Detection System is a pre-processing of a captured traffic scene ahead of vehicle. Main part of this paper contains a description of user friendly software interface for an image pre-processing.

  5. Endocytic machinery protein SlaB is dispensable for polarity establishment but necessary for polarity maintenance in hyphal tip cells of Aspergillus nidulans.

    Science.gov (United States)

    Hervás-Aguilar, América; Peñalva, Miguel A

    2010-10-01

    The Aspergillus nidulans endocytic internalization protein SlaB is essential, in agreement with the key role in apical extension attributed to endocytosis. We constructed, by gene replacement, a nitrate-inducible, ammonium-repressible slaB1 allele for conditional SlaB expression. Video microscopy showed that repressed slaB1 cells are able to establish but unable to maintain a stable polarity axis, arresting growth with budding-yeast-like morphology shortly after initially normal germ tube emergence. Using green fluorescent protein (GFP)-tagged secretory v-SNARE SynA, which continuously recycles to the plasma membrane after being efficiently endocytosed, we establish that SlaB is crucial for endocytosis, although it is dispensable for the anterograde traffic of SynA and of the t-SNARE Pep12 to the plasma and vacuolar membrane, respectively. By confocal microscopy, repressed slaB1 germlings show deep plasma membrane invaginations. Ammonium-to-nitrate medium shift experiments demonstrated reversibility of the null polarity maintenance phenotype and correlation of normal apical extension with resumption of SynA endocytosis. In contrast, SlaB downregulation in hyphae that had progressed far beyond germ tube emergence led to marked polarity maintenance defects correlating with deficient SynA endocytosis. Thus, the strict correlation between abolishment of endocytosis and disability of polarity maintenance that we report here supports the view that hyphal growth requires coupling of secretion and endocytosis. However, downregulated slaB1 cells form F-actin clumps containing the actin-binding protein AbpA, and thus F-actin misregulation cannot be completely disregarded as a possible contributor to defective apical extension. Latrunculin B treatment of SlaB-downregulated tips reduced the formation of AbpA clumps without promoting growth and revealed the formation of cortical "comets" of AbpA.

  6. Road Traffic in China

    NARCIS (Netherlands)

    Jie, L.; Van Zuylen, H.J.

    2014-01-01

    Traffic is tightly related to the social and economic development in a country. In China the development of the economy has been very fast in the past 30 years and this is still continuing. The transport infrastructure shows a similar pattern, while traffic is also rapidly growing. In urban areas

  7. Traffic Light Options

    DEFF Research Database (Denmark)

    Jørgensen, Peter Løchte

    2007-01-01

    This paper introduces, prices, and analyzes traffic light options. The traffic light option is an innovative structured OTC derivative developed independently by several London-based investment banks to suit the needs of Danish life and pension (L&P) companies, which must comply with the traffic...... light solvency stress test system introduced by the Danish Financial Supervisory Authority (DFSA) in June 2001. This monitoring system requires L&P companies to submit regular reports documenting the sensitivity of the companies' base capital to certain pre-defined market shocks - the red and yellow...... light scenarios. These stress scenarios entail drops in interest rates as well as in stock prices, and traffic light options are thus designed to pay off and preserve sufficient capital when interest rates and stock prices fall simultaneously. Sweden's FSA implemented a traffic light system in January...

  8. Traffic planning for non-homogeneous traffic

    Indian Academy of Sciences (India)

    Transport professionals can use these PCU values for accurate capacity, safety, and operational ... Most transportation engineering work depends on the use of continuity equations and pas- senger car units ...... of a service lane can serve slow and local traffic, as a capacity enhancement strategy would have higher benefit ...

  9. VBR video traffic models

    CERN Document Server

    Tanwir, Savera

    2014-01-01

    There has been a phenomenal growth in video applications over the past few years. An accurate traffic model of Variable Bit Rate (VBR) video is necessary for performance evaluation of a network design and for generating synthetic traffic that can be used for benchmarking a network. A large number of models for VBR video traffic have been proposed in the literature for different types of video in the past 20 years. Here, the authors have classified and surveyed these models and have also evaluated the models for H.264 AVC and MVC encoded video and discussed their findings.

  10. Comsat's TDMA traffic terminal

    Science.gov (United States)

    Benjamin, M. C.; Bogaert, W. M.

    1985-06-01

    Comsat has installed two traffic terminals in the Etam earth-station and is currently installing a third in the new Roaring Creek earth-station to access the Intelsat TDMA network. This paper describes the Comsat TDMA traffic terminal equipment from the supergroup interface to the antenna. Comsat's 1: N redundancy approach for terrestrial interface equipment and DSI unit back-up is described as well as electrical path length, amplitude and group delay equalization techniques, special on-line RF monitoring and failure reporting facilities and the operation and maintenance center which can operate and perform diagnostic testing on up to four traffic terminals from a central location.

  11. miR-17-5p regulates endocytic trafficking through targeting TBC1D2/Armus.

    Directory of Open Access Journals (Sweden)

    Andrius Serva

    Full Text Available miRNA cluster miR-17-92 is known as oncomir-1 due to its potent oncogenic function. miR-17-92 is a polycistronic cluster that encodes 6 miRNAs, and can both facilitate and inhibit cell proliferation. Known targets of miRNAs encoded by this cluster are largely regulators of cell cycle progression and apoptosis. Here, we show that miRNAs encoded by this cluster and sharing the seed sequence of miR-17 exert their influence on one of the most essential cellular processes - endocytic trafficking. By mRNA expression analysis we identified that regulation of endocytic trafficking by miR-17 can potentially be achieved by targeting of a number of trafficking regulators. We have thoroughly validated TBC1D2/Armus, a GAP of Rab7 GTPase, as a novel target of miR-17. Our study reveals regulation of endocytic trafficking as a novel function of miR-17, which might act cooperatively with other functions of miR-17 and related miRNAs in health and disease.

  12. Regulation of Src trafficking and activation by the endocytic regulatory proteins MICAL-L1 and EHD1

    Science.gov (United States)

    Reinecke, James B.; Katafiasz, Dawn; Naslavsky, Naava; Caplan, Steve

    2014-01-01

    ABSTRACT Localization of the non-receptor tyrosine kinase Src to the cell periphery is required for its activation and to mediate focal adhesion turnover, cell spreading and migration. Inactive Src localizes to a perinuclear compartment and the movement of Src to the plasma membrane is mediated by endocytic transport. However, the precise pathways and regulatory proteins that are responsible for SRC transport are incompletely understood. Here, we demonstrate that Src partially colocalizes with the endocytic regulatory protein MICAL-L1 (molecule interacting with CasL-like protein 1) in mammalian cells. Furthermore, MICAL-L1 is required for growth-factor- and integrin-induced Src activation and transport to the cell periphery in HeLa cells and human fibroblasts. Accordingly, MICAL-L1 depletion impairs focal adhesion turnover, cell spreading and cell migration. Interestingly, we find that the MICAL-L1 interaction partner EHD1 (EH domain-containing protein 1) is also required for Src activation and transport. Moreover, the MICAL-L1-mediated recruitment of EHD1 to Src-containing recycling endosomes is required for the release of Src from the perinuclear endocytic recycling compartment in response to growth factor stimulation. Our study sheds new light on the mechanism by which Src is transported to the plasma membrane and activated, and provides a new function for MICAL-L1 and EHD1 in the regulation of intracellular non-receptor tyrosine kinases. PMID:24481818

  13. Energy Saving Scheme Based On Traffic Forwarding For Optical Fiber Access Networks

    DEFF Research Database (Denmark)

    Lopez, G. Arturo Rodes; Estaran Tolosa, Jose Manuel; Vegas Olmos, Juan José

    2013-01-01

    We report on an energy saving block that regroups and powers off OLTs during low traffic periods, resulting in energy savings up to 87,5% in the central office of optical access networks.......We report on an energy saving block that regroups and powers off OLTs during low traffic periods, resulting in energy savings up to 87,5% in the central office of optical access networks....

  14. Tourism Traffic Management

    OpenAIRE

    Ioan Cosmescu

    2001-01-01

    Tourism traffic must be quantified through four observing units’ categories: custom houses; quartering units; internal and external travel agencies on home teritory; familiy budgets. These observing units allow to quantify the statistical observation’s object and its cyclicity.

  15. Driver behavior in traffic.

    Science.gov (United States)

    2012-02-01

    Existing traffic analysis and management tools do not model the ability of drivers to recognize their environment and respond to it with behaviors that vary according to the encountered driving situation. The small body of literature on characterizin...

  16. Non-Traffic Citations

    Data.gov (United States)

    Allegheny County / City of Pittsburgh / Western PA Regional Data Center — Non-traffic citations (NTCs, also known as "summary offenses") document low-level criminal offenses where a law enforcement officer or other authorized official...

  17. Allegheny County Traffic Counts

    Data.gov (United States)

    Allegheny County / City of Pittsburgh / Western PA Regional Data Center — Traffic sensors at over 1,200 locations in Allegheny County collect vehicle counts for the Pennsylvania Department of Transportation. Data included in the Health...

  18. Traffic Signal Cycle Lengths

    Data.gov (United States)

    Town of Chapel Hill, North Carolina — Traffic signal location list for the town of Chapel Hill. This data set includes light cycle information as well as as intersection information.The Town of Chapel...

  19. The endocytic recycling regulator EHD1 is essential for spermatogenesis and male fertility in mice

    Directory of Open Access Journals (Sweden)

    Hess Rex A

    2010-04-01

    Full Text Available Abstract Background The C-terminal Eps15 homology domain-containing protein 1 (EHD1 is ubiquitously expressed and regulates the endocytic trafficking and recycling of membrane components and several transmembrane receptors. To elucidate the function of EHD1 in mammalian development, we generated Ehd1-/- mice using a Cre/loxP system. Results Both male and female Ehd1-/- mice survived at sub-Mendelian ratios. A proportion of Ehd1-/- mice were viable and showed smaller size at birth, which continued into adulthood. Ehd1-/- adult males were infertile and displayed decreased testis size, whereas Ehd1-/- females were fertile. In situ hybridization and immunohistochemistry of developing wildtype mouse testes revealed EHD1 expression in most cells of the seminiferous epithelia. Histopathology revealed abnormal spermatogenesis in the seminiferous tubules and the absence of mature spermatozoa in the epididymides of Ehd1-/- males. Seminiferous tubules showed disruption of the normal spermatogenic cycle with abnormal acrosomal development on round spermatids, clumping of acrosomes, misaligned spermatids and the absence of normal elongated spermatids in Ehd1-/- males. Light and electron microscopy analyses indicated that elongated spermatids were abnormally phagocytosed by Sertoli cells in Ehd1-/- mice. Conclusions Contrary to a previous report, these results demonstrate an important role for EHD1 in pre- and post-natal development with a specific role in spermatogenesis.

  20. Role of STARD4 in sterol transport between the endocytic recycling compartment and the plasma membrane.

    Science.gov (United States)

    Iaea, David B; Mao, Shu; Lund, Frederik W; Maxfield, Frederick R

    2017-04-15

    Cholesterol is an essential constituent of membranes in mammalian cells. The plasma membrane and the endocytic recycling compartment (ERC) are both highly enriched in cholesterol. The abundance and distribution of cholesterol among organelles are tightly controlled by a combination of mechanisms involving vesicular and nonvesicular sterol transport processes. Using the fluorescent cholesterol analogue dehydroergosterol, we examined sterol transport between the plasma membrane and the ERC using fluorescence recovery after photobleaching and a novel sterol efflux assay. We found that sterol transport between these organelles in a U2OS cell line has a t 1/2 =12-15 min. Approximately 70% of sterol transport is ATP independent and therefore is nonvesicular. Increasing cellular cholesterol levels dramatically increases bidirectional transport rate constants, but decreases in cholesterol levels have only a modest effect. A soluble sterol transport protein, STARD4, accounts for ∼25% of total sterol transport and ∼33% of nonvesicular sterol transport between the plasma membrane and ERC. This study shows that nonvesicular sterol transport mechanisms and STARD4 in particular account for a large fraction of sterol transport between the plasma membrane and the ERC. © 2017 Iaea et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

  1. Age-related changes in the endocytic capacity of rat liver Kupffer and endothelial cells

    International Nuclear Information System (INIS)

    Brouwer, A.; Barelds, R.J.; Knook, D.L.

    1985-01-01

    There are many indications that the functional capacity of the reticuloendothelial system (RES) declines with age. The aim of this study was to investigate the cellular basis of age-related changes in the clearance function of the RES. The experiments were focused mainly on Kupffer and endothelial cells of the liver which represent a major part of the RES and are primarily responsible for clearance of colloidal material from the circulation. The clearance capacity of the RES was tested clinically and experimentally by intravenous injection of colloids, such as radiolabeled heat-aggregated colloidal albumin. Age-related changes in the endocytosis of 125 I-labeled colloidal albumin (CA) in rats were determined by clearance and organ distribution of different doses of intravenously injected CA, uptake of CA by Kupffer and endothelial liver cells in vivo as determined after isolation of the cells from injected rats and kinetic studies on CA uptake by Kupffer cells in culture. The results show that, at a low dose, the clearance of CA is primarily determined by liver blood flow. At a higher saturating dose, plasma clearance and uptake by the liver are not significantly decreased with age. Endocytosis by endothelial cells, which accounts for about 60% of that of the whole liver, is also unchanged with age. In contrast, a significant decrease in endocytic capacity was observed for Kupffer cells in vivo. This age-related functional decline was also observed in Kupffer cells which were isolated from rats of different ages and maintained in culture

  2. Endocytic regulation of alkali metal transport proteins in mammals, yeast and plants.

    Science.gov (United States)

    Mulet, José Miguel; Llopis-Torregrosa, Vicent; Primo, Cecilia; Marqués, Ma Carmen; Yenush, Lynne

    2013-11-01

    The relative concentrations of ions and solutes inside cells are actively maintained by several classes of transport proteins, in many cases against their concentration gradient. These transport processes, which consume a large portion of cellular energy, must be constantly regulated. Many structurally distinct families of channels, carriers, and pumps have been characterized in considerable detail during the past decades and defects in the function of some of these proteins have been linked to a growing list of human diseases. The dynamic regulation of the transport proteins present at the cell surface is vital for both normal cellular function and for the successful adaptation to changing environments. The composition of proteins present at the cell surface is controlled on both the transcriptional and post-translational level. Post-translational regulation involves highly conserved mechanisms of phosphorylation- and ubiquitylation-dependent signal transduction routes used to modify the cohort of receptors and transport proteins present under any given circumstances. In this review, we will summarize what is currently known about one facet of this regulatory process: the endocytic regulation of alkali metal transport proteins. The physiological relevance, major contributors, parallels and missing pieces of the puzzle in mammals, yeast and plants will be discussed.

  3. Effects of traffic infrastructure and road design on safe driving

    NARCIS (Netherlands)

    Horst, A.R.A. van der

    2014-01-01

    One of the building blocks of the Dutch success in road safety is the Sustainable Safety approach. This philosophy specifies that road safety should be a design requirement in road traffic with a focus on the use of human characteristics as a starting point, considering both humans’ physical

  4. Traffic pollution and countermeasures of urban traffic environment

    Science.gov (United States)

    He, Yuhong; Zheng, Chaocheng

    2018-01-01

    Background: Traffic environment has become a serious social problem in China currently, therefore, urban traffic environment governance is the requirement to solve this issue because as an important place in people's social life, urban traffic environment shows a strong city's energy. Objective: Based on analysis on social function of city traffic environment and its influence of traffic on urban environment in this paper, the goal to establish a healthy urban traffic environment must be included under the aim of sustainable development eternally and feasible measures were put forward afterwards. Method, result, conclusion and possible applications.

  5. Targeted disruption of core 1 β1,3-galactosyltransferase (C1galt1 induces apical endocytic trafficking in human corneal keratinocytes.

    Directory of Open Access Journals (Sweden)

    Ana Guzman-Aranguez

    Full Text Available BACKGROUND: Exposed mucosal surfaces limit constitutive endocytosis under physiological conditions to prevent uptake of macromolecules and pathogens and, therefore, cellular damage. It is now accepted that cell surface mucins, a group of high molecular weight glycoproteins on the epithelial glycocalyx, defined by their extensive O-glycosylation, play a major role in maintaining barrier function in these surfaces, but the precise mechanisms are unclear. METHODOLOGY/PRINCIPAL FINDINGS: In this work, we utilized a stable tetracycline-inducible RNA interfering system targeting the core 1 ß1,3-galactosyltransferase (C1galt1 or T-synthase, a critical galactosyltransferase required for the synthesis of core 1 O-glycans, to explore the role of mucin-type carbohydrates in apical endocytic trafficking in human corneal keratinocytes. Using cell surface biotinylation and subcellular fractionation, we found increased accumulation of plasma membrane protein in endosomes after C1galt1 depletion. Confocal laser scanning microscopy and fluorometry revealed increased translocation of negatively charged fluorescent nanospheres after C1galt1 knockdown sustained by an active transport process and largely independent of apical intercellular junctions. Internalization of nanospheres could be blocked by dynasore, nocodazole, chlorpromazine, and hyperosmotic sucrose, suggesting a mechanism for clathrin-coated pit budding and vesicular trafficking. This possibility was supported by experiments showing nanosphere colocalization with clathrin heavy chain in the cytoplasm. CONCLUSIONS/SIGNIFICANCE: Together, the data suggest that core 1 O-glycans contribute to maintenance of apical barrier function on exposed mucosal surfaces by preventing clathrin-mediated endocytosis.

  6. Trip-timing decisions with traffic incidents

    DEFF Research Database (Denmark)

    Fosgerau, Mogens; Lindsey, Robin

    2013-01-01

    This paper analyzes traffic bottleneck congestion when drivers randomly cause incidents that temporarily block the bottleneck. Drivers have general scheduling preferences for time spent at home and at work. They independently choose morning departure times from home to maximize expected utility...... without knowing whether an incident has occurred. The resulting departure time pattern may be compressed or dispersed according to whether or not the bottleneck is fully utilized throughout the departure period on days without incidents. For both the user equilibrium (UE) and the social optimum (SO...

  7. Ultrasound guided supraclavicular block.

    LENUS (Irish Health Repository)

    Hanumanthaiah, Deepak

    2013-09-01

    Ultrasound guided regional anaesthesia is becoming increasingly popular. The supraclavicular block has been transformed by ultrasound guidance into a potentially safe superficial block. We reviewed the techniques of performing supraclavicular block with special focus on ultrasound guidance.

  8. Intelligent Traffic Quantification System

    Science.gov (United States)

    Mohanty, Anita; Bhanja, Urmila; Mahapatra, Sudipta

    2017-08-01

    Currently, city traffic monitoring and controlling is a big issue in almost all cities worldwide. Vehicular ad-hoc Network (VANET) technique is an efficient tool to minimize this problem. Usually, different types of on board sensors are installed in vehicles to generate messages characterized by different vehicle parameters. In this work, an intelligent system based on fuzzy clustering technique is developed to reduce the number of individual messages by extracting important features from the messages of a vehicle. Therefore, the proposed fuzzy clustering technique reduces the traffic load of the network. The technique also reduces congestion and quantifies congestion.

  9. Traffic speed management

    Directory of Open Access Journals (Sweden)

    Subotić Jovana Lj.

    2014-01-01

    Full Text Available Speed, and vehicles themselves, affect the level of service and road safety, quality of life, noise from traffic, the environment, health, air pollution, emission of carbon dioxide, global warming, the economy and consumption of non-renewable energy such as oil. Therefore, the speed management of the traffic of multiple significance and that should be primarily to provide effective and economical conditions of the modern and preventive protection of human life as the greatest treasure and then the material resources. The way to accomplish this is by using various (different measures such as: appropriate planning and projecting roads and streets, speed control, the legislation, enforcement, campaigns, education, advanced technologies (ITS.

  10. 2016 Traffic Safety Culture Index

    Science.gov (United States)

    ... SEARCH Driver Behavior & Performance 2016 Traffic Safety Culture Index This report presents the results of our annual Traffic Safety Culture Index survey, providing data on the attitudes and behaviors ...

  11. Texas traffic thermostat marketing package.

    Science.gov (United States)

    2013-04-01

    The traffic thermostat decision tool is built to help guide the user through a logical, step-wise, process of examining potential changes to their Manage Lane/toll facility. : **NOTE: Project Title: Application of the Traffic Thermostat Framework. Ap...

  12. Texas traffic thermostat software tool.

    Science.gov (United States)

    2013-04-01

    The traffic thermostat decision tool is built to help guide the user through a logical, step-wise, process of examining potential changes to their Manage Lane/toll facility. : **NOTE: Project Title: Application of the Traffic Thermostat Framework. Ap...

  13. Traffic Engineering in Metro Ethernet

    OpenAIRE

    Padmaraj Nair; Suku Nair; Girish Chiruvolu

    2010-01-01

    Traffic engineering is one of the major issues that has to be addressed in Metro Ethernet networks for quality of service and efficient resource utilization. This paper aims at understanding the relevant issues and outlines novel algorithms for multipoint traffic engineering in Metro Ethernet. We present an algorithmic solution for traffic engineering in Metro Ethernet using optimal multiple spanning trees. This iterative approach distributes traffic across the network uniformly without overl...

  14. Framework for Traffic Congestion Prediction

    NARCIS (Netherlands)

    Zaki, J.F.W.; Ali-Eldin, A.M.T.; Hussein, S.E.; Saraya, S.F.; Areed, F.F.

    2016-01-01

    Traffic Congestion is a complex dilemma facing most major cities. It has undergone a lot of research since the early 80s in an attempt to predict traffic in the short-term. Recently, Intelligent Transportation Systems (ITS) became an integral part of traffic research which helped in modeling and

  15. Traffic light intensity meter, TIM®

    NARCIS (Netherlands)

    Leden, N. van der; Varkevisser, J.; Vroom, J. de; Oijen, T van

    2005-01-01

    The intensity of traffic lights decreases over time as a result of pollution and ageing. The Dutch Traffic Research Centre of the Ministry of Transport, Public Works and Water Management is searching for a convenient method for measuring the luminous intensity of traffic lights on the road, in order

  16. Traffic Flow Visualization and Control

    National Research Council Canada - National Science Library

    Larson, Robert

    1999-01-01

    .... Vehicle count, flow speed, headway, queue length and occupancy are some of the information that can be collected. The processed traffic data is then sent to a control center for further analysis and used by traffic operators attempting to analyze traffic on the highway.

  17. Traffic sign detection and analysis

    DEFF Research Database (Denmark)

    Møgelmose, Andreas; Trivedi, Mohan M.; Moeslund, Thomas B.

    2012-01-01

    Traffic sign recognition (TSR) is a research field that has seen much activity in the recent decade. This paper introduces the problem and presents 4 recent papers on traffic sign detection and 4 recent papers on traffic sign classification. It attempts to extract recent trends in the field and t...

  18. The Classroom Traffic Jam

    Science.gov (United States)

    Edwards, Arthur W.

    1977-01-01

    The importance of energy conservation is developed in this simulation. Children draw an automobile and then are asked to drive it through the classroom roadways. When a traffic jam results, students offer ways to eliminate it. The importance of mass transportation and car pools is stressed by the teacher. (MA)

  19. Inaccuracy in traffic forecasts

    DEFF Research Database (Denmark)

    Flyvbjerg, Bent; Holm, Mette K. Skamris; Buhl, Søren Ladegaard

    2006-01-01

    , the difference between actual and forecasted traffic is more than +-20%; for 25% of road projects, the difference is larger than +-40%. Forecasts for roads are more accurate and more balanced than for rail, with no significant difference between the frequency of inflated versus deflated forecasts. But for both...

  20. Alcohol and Traffic Safety.

    Science.gov (United States)

    Dickman, Frances Baker, Ed.

    1988-01-01

    Seven papers discuss current issues and applied social research concerning alcohol traffic safety. Prevention, policy input, methodology, planning strategies, anti-drinking/driving programs, social-programmatic orientations of Mothers Against Drunk Driving, Kansas Driving Under the Influence Law, New Jersey Driving While Impaired Programs,…

  1. Impact of traffic noise on railway traffic safety

    Directory of Open Access Journals (Sweden)

    Zdravko TOŠ

    2008-01-01

    Full Text Available Traffic noise is one of the dominant factors of ergo-assessment. The harmful impact of traffic noise on the engine driver as target group can be studied in isolation from other ergo-assessment factors only in the initial phase of research. The simultaneous action of several related factors in the system of ergo-assessment factors has cumulative effect on the perception and psychomotoric status of the railway traffic participants in the appropriate traffic situation. The initial partial research of traffic noise by a combination of several scientific methods needs to be eventually upgraded by studying the relations among several concurrent important or dominant ergo-assessment factors.

  2. Stochastic Model of Traffic Jam and Traffic Signal Control

    Science.gov (United States)

    Shin, Ji-Sun; Cui, Cheng-You; Lee, Tae-Hong; Lee, Hee-Hyol

    Traffic signal control is an effective method to solve the traffic jam. and forecasting traffic density has been known as an important part of the Intelligent Transportation System (ITS). The several methods of the traffic signal control are known such as random walk method, Neuron Network method, Bayesian Network method, and so on. In this paper, we propose a new method of a traffic signal control using a predicted distribution of traffic jam based on a Dynamic Bayesian Network model. First, a forecasting model to predict a probabilistic distribution of the traffic jam during each period of traffic lights is built. As the forecasting model, the Dynamic Bayesian Network is used to predict the probabilistic distribution of a density of the traffic jam. According to measurement of two crossing points for each cycle, the inflow and outflow of each direction and the number of standing vehicles at former cycle are obtained. The number of standing vehicle at k-th cycle will be calculated synchronously. Next, the probabilistic distribution of the density of standing vehicle in each cycle will be predicted using the Dynamic Bayesian Network constructed for the traffic jam. And then a control rule to adjust the split and the cycle to increase the probability between a lower limit and ceiling of the standing vehicles is deduced. As the results of the simulation using the actual traffic data of Kitakyushu city, the effectiveness of the method is shown.

  3. Homogeneous bilateral block shifts

    Indian Academy of Sciences (India)

    Douglas class were classified in [3]; they are unilateral block shifts of arbitrary block size (i.e. dim H(n) can be anything). However, no examples of irreducible homogeneous bilateral block shifts of block size larger than 1 were known until now.

  4. Smart Traffic Management Protocol Based on VANET architecture

    Directory of Open Access Journals (Sweden)

    Amilcare Francesco Santamaria

    2014-01-01

    Full Text Available Nowadays one of the hottest theme in wireless environments research is the application of the newest technologies to road safety problems and traffic management exploiting the (VANET architecture. In this work, a novel protocol that aims to achieve a better traffic management is proposed. The overal system is able to reduce traffic level inside the city exploiting inter-communication among vehicles and support infrastructures also known as (V2V and (V2I communications. We design a network protocol called (STMP that takes advantages of IEEE 802.11p standard. On each road several sensors system are placed and they are responsible of monitoring. Gathered data are spread in the network exploiting ad-hoc protocol messages. The increasing knowledge about environment conditions make possible to take preventive actions. Moreover, having a realtime monitoring of the lanes it is possible to reveal roads and city blocks congestions in a shorter time. An external entity to the (VANET is responsible to manage traffic and rearrange traffic along the lanes of the city avoiding huge traffic levels.

  5. Regulation of the V-ATPase along the endocytic pathway occurs through reversible subunit association and membrane localization.

    Directory of Open Access Journals (Sweden)

    Céline Lafourcade

    2008-07-01

    Full Text Available The lumen of endosomal organelles becomes increasingly acidic when going from the cell surface to lysosomes. Luminal pH thereby regulates important processes such as the release of internalized ligands from their receptor or the activation of lysosomal enzymes. The main player in endosomal acidification is the vacuolar ATPase (V-ATPase, a multi-subunit transmembrane complex that pumps protons from the cytoplasm to the lumen of organelles, or to the outside of the cell. The active V-ATPase is composed of two multi-subunit domains, the transmembrane V(0 and the cytoplasmic V(1. Here we found that the ratio of membrane associated V(1/Vo varies along the endocytic pathway, the relative abundance of V(1 being higher on late endosomes than on early endosomes, providing an explanation for the higher acidity of late endosomes. We also found that all membrane-bound V-ATPase subunits were associated with detergent resistant membranes (DRM isolated from late endosomes, raising the possibility that association with lipid-raft like domains also plays a role in regulating the activity of the proton pump. In support of this, we found that treatment of cells with U18666A, a drug that leads to the accumulation of cholesterol in late endosomes, affected acidification of late endosome. Altogether our findings indicate that the activity of the vATPase in the endocytic pathway is regulated both by reversible association/dissociation and the interaction with specific lipid environments.

  6. Estimation of Carbon Dioxide Emissions Generated by Building and Traffic in Taichung City

    Directory of Open Access Journals (Sweden)

    Chou-Tsang Chang

    2018-01-01

    Full Text Available The emissions of carbon dioxide generated by urban traffic is generally reflected by urban size. In order to discuss the traffic volume generated in developed buildings and road crossings in a single urban block, with the metropolitan area in Taichung, Taiwan as an example, this study calculates the mutual relationship between the carbon dioxide generated by the traffic volume and building development scale, in order to research energy consumption and relevance. In this research, the entire-day traffic volume of an important road crossing is subject to statistical analysis to obtain the prediction formula of total passenger car units in the main road crossing within 24 h. Then, the total CO2 emissions generated by the traffic volume in the entire year is calculated according to the investigation data of peak traffic hours within 16 blocks and the influential factors of the development scale of 95 buildings are counted. Finally, this research found that there is a passenger car unit of 4.72 generated in each square meter of land in the urban block every day, 0.99 in each square meter of floor area in the building and the average annual total CO2 emissions of each passenger car unit is 41.4 kgCO2/yr. In addition, the basic information of an integrated road system and traffic volume is used to present a readable urban traffic hot map, which can calculate a distribution map of passenger car units within one day in Taichung. This research unit can be used to forecast the development scale of various buildings in future urban blocks, in order to provide an effective approach to estimate the carbon dioxide generated by the traffic volume.

  7. Traffic Light Detection

    DEFF Research Database (Denmark)

    Philipsen, Mark Philip; Jensen, Morten Bornø; Møgelmose, Andreas

    2015-01-01

    Traffic light recognition (TLR) is an integral part of any intelligent vehicle, which must function in the existing infrastructure. Pedestrian and sign detection have recently seen great improvements due to the introduction of learning based detectors using integral channel features. A similar push...... have not yet been seen for the detection sub-problem of TLR, where detection is dominated by methods based on heuristic models. Evaluation of existing systems is currently limited primarily to small local datasets. In order to provide a common basis for comparing future TLR research an extensive public...... database is collected based on footage from US roads. The database consists of both test and training data, totaling 46,418 frames and 112,971 annotated traffic lights, captured in continuous sequences under a varying light and weather conditions. The learning based detector achieves an AUC of 0.4 and 0...

  8. Physics of Traffic Flow

    Science.gov (United States)

    Davis, L. C.

    2015-03-01

    The Texas A&M Transportation Institute estimated that traffic congestion cost the United States 121 billion in 2011 (the latest data available). The cost is due to wasted time and fuel. In addition to accidents and road construction, factors contributing to congestion include large demand, instability of high-density free flow and selfish behavior of drivers, which produces self-organized traffic bottlenecks. Extensive data collected on instrumented highways in various countries have led to a better understanding of traffic dynamics. From these measurements, Boris Kerner and colleagues developed a new theory called three-phase theory. They identified three major phases of flow observed in the data: free flow, synchronous flow and wide moving jams. The intermediate phase is called synchronous because vehicles in different lanes tend to have similar velocities. This congested phase, characterized by lower velocities yet modestly high throughput, frequently occurs near on-ramps and lane reductions. At present there are only two widely used methods of congestion mitigation: ramp metering and the display of current travel-time information to drivers. To find more effective methods to reduce congestion, researchers perform large-scale simulations using models based on the new theories. An algorithm has been proposed to realize Wardrop equilibria with real-time route information. Such equilibria have equal travel time on alternative routes between a given origin and destination. An active area of current research is the dynamics of connected vehicles, which communicate wirelessly with other vehicles and the surrounding infrastructure. These systems show great promise for improving traffic flow and safety.

  9. Modeling of Spacewire Traffic

    Science.gov (United States)

    Liebgott, Emmanuelle; Fourtier, Philippe; Jameux, David

    2011-08-01

    The Spacewire technology allows embarking high speed data networks on board spacecraft and become widely adopted by agencies and industries missions. Need for new tool to support conception, development and validation of such high speed data network has increased with Spacewire standard development. In that frame, MOST is a representative traffic spacewire simulator which presents great interests for both system engineers and spacewire experts:• It offers the possibility to build SpW network model, selecting and configuring SpW components, and to test defined design without waiting for HW,• It allows to keep control on traffic load and identify weak parts of the network topology,• It gives load margins and traffic performances,• It allows failures simulation and give the possibility to run various scenarios• MOST supports design risks decrease and secures planning thanks to early verification,• In addition, MOST is a progressive tool which allows testing of SpW standard evolutions.

  10. Assessing Road Traffic Expression

    Directory of Open Access Journals (Sweden)

    Fábio Silva

    2014-12-01

    Full Text Available Road traffic is a problem which is increasing in cities with large population. Unrelated to this fact the number of portable and wearable devices has also been increasing throughout the population of most countries. With this advent, the capacity to monitor and register data about people habits and locations as well as more complex data such as intensity and strength of movements has created an opportunity to contribute to the general wealth and comfort within these environments. Ambient Intelligence and Intelligent Decision Making processes can benefit from the knowledge gathered by these devices to improve decisions on everyday tasks such as deciding navigation routes by car, bicycle or other means of transportation and avoiding route perils. The concept of computational sustainability may also be applied to this problem. Current applications in this area demonstrate the usefulness of real time system that inform the user of certain conditions in the surrounding area. On the other hand, the approach presented in this work aims to describe models and approaches to automatically identify current states of traffic inside cities and use methods from computer science to improve overall comfort and the sustainability of road traffic both with the user and the environment in mind. Such objective is delivered by analyzing real time contributions from those mobile ubiquitous devices to identifying problematic situations and areas under a defined criteria that have significant influence towards a sustainable use of the road transport infrastructure.

  11. Effects of traffic noise

    Energy Technology Data Exchange (ETDEWEB)

    Gottlob, D.

    1986-02-01

    One of the main sources of noise is road traffic. In 1984 there were over 25 million cars, 1.2 million lorries, 1.3 million motor cycles and 1.6 million mopeds using our roads. Opinion polls showed that 21% of the population felt that they were affected by traffic noise as a nuisance factor. An outline of the effects of this noise on the affected population is given, illustrated by diagrams. Details about noise emissions (drive-past level) of the different types of vehicles in city traffic are stated and the effects of noise described. The author goes into the nuisance effect (noise is not a physical factor, but a psychosocial one), changes in behaviour (ways of speaking, reduction of stress on households in proportion to rising income and higher educational levels) and the consequences for health (the reaction of the body to noise is primarily a consequence of the psychosomatic organisation of ow bodies). In conclusion, the author deals with the subjective efficiency of noise protection measures. (HWJ).

  12. Compliance with traffic laws by traffic police officers, non-traffic police officers, and civilian drivers.

    Science.gov (United States)

    Rosenbloom, Tova; Pereg, Avihu; Perlman, Amotz

    2014-01-01

    The policy of a public organization, such as police, may shape the norms and the behavior of the citizens. In line with this, police officers are expected by the public to comply with traffic laws and serve as an example for the citizenry. This study used on-site observations of civilian and police driver, comparing police officers' compliance with traffic laws to that of civilians. We compared driver compliance with traffic laws for drivers in 3 groups of vehicles: traffic police cars, non-traffic police cars, and civilian cars. Four hundred sixty-six vehicles were observed and compared by vehicle type and whether a uniform was worn by the driver. We observed safety belt usage, signaling before turning, cellular phone usage, and giving way to traffic (measured by merging time). We found evidence that generally drivers in police cars use seat belts while driving more that drivers in civilian cars do. In particular, more traffic police car drivers used seat belts than non-traffic police car drivers do. In addition, drivers in civilian cars and non-traffic police cars waited longer periods of time before merging right into traffic compared to traffic police car drivers. Our findings supported the notion that on-duty police officers, and traffic police officers in particular, adhere more closely to traffic laws compared to civilian drivers. As the general public compliance with traffic laws is affected by the police perceived legitimacy, the publication of these results can both boost public cooperation with the police and encourage police officers to continue providing positive role models to the public.

  13. Reciprocal expression of the endocytic protein HIP1R and its repressor FOXP1 predicts outcome in R-CHOP-treated diffuse large B-cell lymphoma patients

    DEFF Research Database (Denmark)

    Wong, K K; Gascoyne, D M; Brown, P J

    2014-01-01

    We previously identified autoantibodies to the endocytic-associated protein Huntingtin-interacting protein 1-related (HIP1R) in diffuse large B-cell lymphoma (DLBCL) patients. HIP1R regulates internalization of cell surface receptors via endocytosis, a process relevant to many therapeutic strateg...

  14. Traffic Games: Modeling Freeway Traffic with Game Theory.

    Science.gov (United States)

    Cortés-Berrueco, Luis E; Gershenson, Carlos; Stephens, Christopher R

    2016-01-01

    We apply game theory to a vehicular traffic model to study the effect of driver strategies on traffic flow. The resulting model inherits the realistic dynamics achieved by a two-lane traffic model and aims to incorporate phenomena caused by driver-driver interactions. To achieve this goal, a game-theoretic description of driver interaction was developed. This game-theoretic formalization allows one to model different lane-changing behaviors and to keep track of mobility performance. We simulate the evolution of cooperation, traffic flow, and mobility performance for different modeled behaviors. The analysis of these results indicates a mobility optimization process achieved by drivers' interactions.

  15. Photocatalytic pavement blocks. Air purification by pavement blocks. Final results of the research at BRRC

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2009-07-01

    The use of materials can influence to a large extent the environmental impact of traffic and of road infrastructure. Especially in urban areas, where the risk on smog formation during hot summer days is high, the use of photocatalytic pavement blocks can reduce the air pollution significantly. A project on environmental friendly concrete pavement blocks is conducted at the Belgian Road Research Centre. The use of photocatalytic material in the surface of pavement blocks to obtain air purifying materials is investigated. In contact with light, TiO2 as photocatalyst, is able to reduce the NO and NO2 content in the air, caused by the exhaust of traffic. The efficiency is tested on pavement blocks, but the technique can as well be applied on other road elements (e.g. noise reducing walls, linear elements) or as a coating on new materials or existing structures. At the previous TRA conference in Gotenborgh, Sweden, the principle of photocatalysis was presented. In this paper, emphasis will be put on the final results of the 4-year project obtained in laboratory as well as on site at the Leien of Antwerp (10,000 m{sup 2}). The results indicate a durable efficiency towards NOx reduction, which is in favour for the diminishing of the risk on ozone formation. However, the precise translation from the laboratory towards the site is still in question. The results obtained during the project are discussed in this paper.

  16. Homogeneous bilateral block shifts

    Indian Academy of Sciences (India)

    A new 3-parameter family of homogeneous 2-by-2 block shifts is described. These are the first examples of irreducible homogeneous bilateral block shifts of block size larger than 1. Author Affiliations. Adam Korányi1. Department of Mathematics, The Graduate Center, City University of New York, New York, NY 10016, USA ...

  17. OPTIMASI BANDWITH MENGGUNAKAN TRAFFIC SHAPPING

    Directory of Open Access Journals (Sweden)

    Imam Riadi

    2010-01-01

    Full Text Available Perkembangan layanan komunikasi telah berkembang sangat pesat. Salah satunya adalah pemanfaatan penggunaan bandwith untuk mengakases jaringan Internet. Traffic shapping bandwidth dapat memberikan efesiensi dalam hal pemanfaatan bandwidth pada instansi yang melakukan manajemen dalam lalulintas jaringannya. Metode yang digunakan dalam penelitian ini adalah metode literatur yaitu metode pengumpulan data yang dilakukan dengan mengumpulkan sumber- sumber data yang terkait dan metode eksperimen yaitu melakukan penelitian dengan mengkonfigurasi Mikrotik RouterOS untuk melakukan traffic shapping bandwidth. Hasil penelitian ini adalah router yang dapat melakukan traffic shapping sehingga kualitas koneksi menjadi lebih baik untuk mengakses dan mengambil data dari sebuah website. Kata kunci : Optimasi, Bandwith, Traffic Shapping, MikroTik. 

  18. Bicycle traffic in urban areas

    Directory of Open Access Journals (Sweden)

    Anđelković Zorica

    2015-01-01

    Full Text Available Cycling is a term describing the use of bicycles, but also any mean of transport driven solely by human power. Development of bicycle traffic in urban areas involves construction of cycling infrastructure, adapting streets and other traffic infrastructure to a form suitable for cycling and other means of transport (individual motorized traffic, public transport, walking, ensuring the adequate budget and systematic planning and development of sustainable transport in cities. The paper presents basic settings and conditions as input elements to plan bicycle traffic in urban areas, as well as program- design conditions which lead the activities of planners and designers of urban roads in connection with cyclists.

  19. Inaccuracy in traffic forecasts

    DEFF Research Database (Denmark)

    Flyvbjerg, Bent; Holm, Mette K. Skamris; Buhl, Søren Ladegaard

    2006-01-01

    that forecasters generally do a poor job of estimating the demand for transportation infrastructure projects. The result is substantial downside financial and economic risk. Forecasts have not become more accurate over the 30-year period studied. If techniques and skills for arriving at accurate demand forecasts...... forecasting. Highly inaccurate traffic forecasts combined with large standard deviations translate into large financial and economic risks. But such risks are typically ignored or downplayed by planners and decision-makers, to the detriment of social and economic welfare. The paper presents the data...

  20. Traffic simulation for mixed traffic systems | Mbam | Global Journal of ...

    African Journals Online (AJOL)

    Traffic problem is classified into single and mixed, especially in most developing countries, where motorbikes are used as the most popular transportation system. The aim of this paper is to introduce the motorbike symbol into the traffic light control system to separate cars/lorries indicator from that of motorbike. This is likely ...

  1. Real time traffic models, decision support for traffic management

    NARCIS (Netherlands)

    Wismans, Luc Johannes Josephus; de Romph, E.; Friso, K.; Zantema, K.

    2014-01-01

    Reliable and accurate short-term traffic state prediction can improve the performance of real-time traffic management systems significantly. Using this short-time prediction based on current measurements delivered by advanced surveillance systems will support decision-making processes on various

  2. Real Time Traffic Models, Decision Support for Traffic Management

    NARCIS (Netherlands)

    Wismans, L.; De Romph, E.; Friso, K.; Zantema, K.

    2014-01-01

    Reliable and accurate short-term traffic state prediction can improve the performance of real-time traffic management systems significantly. Using this short-time prediction based on current measurements delivered by advanced surveillance systems will support decision-making processes on various

  3. Traffic Infrastructure in the Development of the Croatian Traffic System

    Directory of Open Access Journals (Sweden)

    Damir Šimulčik

    2012-10-01

    Full Text Available The absence of a long-term traffic policy and of the policyof financing the constntction and maintenance of traffic infrastructurefacilities, represents a synthesis of numerous unresolvedrelations whose negative effects are felt in the overalleconomic and traffic development and consequently theevaluation of national potentials in the field. Adverse aspectcaused by the lack of a clear and feasible policy of financing thetraffic infrastructure facilities, is also a result of not having definedan adequate traffic policy, programme and strategiccourses of development, nor financing models that would be inaccordance with the market and economy system.This indicates that it is necessary to determine a policy forfinancing the constntction and maintenance of traffic infrastntcture,which has to be based on scientific development,team work, availability of plans and programmes to scientistsand experts, determined methodology based on marketing andeconomic logic in defining the programme and strategic tasksand assignments so as to make them feasible.In the near future, intensive preparations for investments inthe overall traffic sysiem are necessary, especially regarding thetraffic infrastntcture facilities - the pivotal points in the processof evaluating the traffic in our national tenitory. Croatia needsto define clearly its strategy in constructing and maintaining thegeneral traffic infrastructure, appointing at the same time thosewho will carry out the given tasks.

  4. Porcine sialoadhesin (CD169/Siglec-1 is an endocytic receptor that allows targeted delivery of toxins and antigens to macrophages.

    Directory of Open Access Journals (Sweden)

    Peter L Delputte

    Full Text Available Sialoadhesin is exclusively expressed on specific subpopulations of macrophages. Since sialoadhesin-positive macrophages are involved in inflammatory autoimmune diseases, such as multiple sclerosis, and potentially in the generation of immune responses, targeted delivery of drugs, toxins or antigens via sialoadhesin-specific immunoconjugates may prove a useful therapeutic strategy. Originally, sialoadhesin was characterized as a lymphocyte adhesion molecule, though recently its involvement in internalization of sialic acid carrying pathogens was shown, suggesting that sialoadhesin is an endocytic receptor. In this report, we show that porcine sialoadhesin-specific antibodies and F(ab'₂ fragments trigger sialoadhesin internalization, both in primary porcine macrophages and in cells expressing recombinant porcine sialoadhesin. Using chemical inhibitors, double immunofluorescence stainings and dominant-negative constructs, porcine sialoadhesin internalization was shown to be clathrin- and Eps15-dependent and to result in targeting to early endosomes but not lysosomes. Besides characterizing the sialoadhesin endocytosis mechanism, two sialoadhesin-specific immunoconjugates were evaluated. We observed that porcine sialoadhesin-specific immunotoxins efficiently kill sialoadhesin-expressing macrophages. Furthermore, porcine sialoadhesin-specific albumin immunoconjugates were shown to be internalized in macrophages and immunization with these immunoconjugates resulted in a rapid and robust induction of albumin-specific antibodies, this compared to immunization with albumin alone. Together, these data expand sialoadhesin functionality and show that it can function as an endocytic receptor, a feature that cannot only be misused by sialic acid carrying pathogens, but that may also be used for specific targeting of toxins or antigens to sialoadhesin-expressing macrophages.

  5. Endocytic mechanism of internalization of dietary peptide lunasin into macrophages in inflammatory condition associated with cardiovascular disease.

    Directory of Open Access Journals (Sweden)

    Anthony Cam

    Full Text Available Cardiovascular disease (CVD is the leading cause of death in the United States. Diet influences risk factors associated with CVD and atherosclerosis, a major vascular disease that arises from inflammation. Lunasin, a peptide derived from plant foods such as soybeans, contains a unique Arg-Gly-Asp cell-adhesion motif and inhibits the pathways involved in the inflammatory cascade. The objective was to determine the mechanism by which lunasin is internalized into human THP-1 macrophages, investigate the expression of endocytic membrane proteins in inflammatory conditions and to identify the pathways involved. While lipopolysaccharide (10 nM, vitronectin (130 nM and a combination of these two molecules enhanced lunasin uptake and increased basal αVβ3 integrin expression, lunasin reduced αVβ3 expression by 25.5, 26.8 and 49.2%, respectively. The pretreatment of cells with brefeldin A (71 µM, an inhibitor of protein trafficking, inhibited lunasin internalization by up to 99.8%. Lunasin increased caveolin-1 expression by up to 204.8%, but did not modulate clathrin. The pretreatment of macrophages with nystatin (54 µM, an inhibitor of caveolae-dependent endocytosis, reduced lunasin internalization. The presence of amantadine (1 mM and amiloride (1 mM, inhibitors of clathrin-mediated endocytosis and macropinocytosis, abolished lunasin cell entry. Lunasin elicited a transient reduction in intracellular levels of Ca²⁺ in LPS-induced macrophages. The results suggest that internalization of lunasin into macrophages is amplified in inflammatory conditions and is primarily mediated by endocytic mechanisms that involve integrin signaling, clathrin-coated structures and macropinosomes. Lunasin may be responsible for attenuation of CVD risk factors by interacting with pathways involved in endocytosis and inflammation.

  6. Endocytic mechanism of internalization of dietary peptide lunasin into macrophages in inflammatory condition associated with cardiovascular disease.

    Science.gov (United States)

    Cam, Anthony; Sivaguru, Mayandi; Gonzalez de Mejia, Elvira

    2013-01-01

    Cardiovascular disease (CVD) is the leading cause of death in the United States. Diet influences risk factors associated with CVD and atherosclerosis, a major vascular disease that arises from inflammation. Lunasin, a peptide derived from plant foods such as soybeans, contains a unique Arg-Gly-Asp cell-adhesion motif and inhibits the pathways involved in the inflammatory cascade. The objective was to determine the mechanism by which lunasin is internalized into human THP-1 macrophages, investigate the expression of endocytic membrane proteins in inflammatory conditions and to identify the pathways involved. While lipopolysaccharide (10 nM), vitronectin (130 nM) and a combination of these two molecules enhanced lunasin uptake and increased basal αVβ3 integrin expression, lunasin reduced αVβ3 expression by 25.5, 26.8 and 49.2%, respectively. The pretreatment of cells with brefeldin A (71 µM), an inhibitor of protein trafficking, inhibited lunasin internalization by up to 99.8%. Lunasin increased caveolin-1 expression by up to 204.8%, but did not modulate clathrin. The pretreatment of macrophages with nystatin (54 µM), an inhibitor of caveolae-dependent endocytosis, reduced lunasin internalization. The presence of amantadine (1 mM) and amiloride (1 mM), inhibitors of clathrin-mediated endocytosis and macropinocytosis, abolished lunasin cell entry. Lunasin elicited a transient reduction in intracellular levels of Ca²⁺ in LPS-induced macrophages. The results suggest that internalization of lunasin into macrophages is amplified in inflammatory conditions and is primarily mediated by endocytic mechanisms that involve integrin signaling, clathrin-coated structures and macropinosomes. Lunasin may be responsible for attenuation of CVD risk factors by interacting with pathways involved in endocytosis and inflammation.

  7. Interaction of the Human Papillomavirus E6 Oncoprotein with Sorting Nexin 27 Modulates Endocytic Cargo Transport Pathways.

    Directory of Open Access Journals (Sweden)

    Ketaki Ganti

    2016-09-01

    Full Text Available A subset of high-risk Human Papillomaviruses (HPVs are the causative agents of a large number of human cancers, of which cervical is the most common. Two viral oncoproteins, E6 and E7, contribute directly towards the development and maintenance of malignancy. A characteristic feature of the E6 oncoproteins from cancer-causing HPV types is the presence of a PDZ binding motif (PBM at its C-terminus, which confers interaction with cellular proteins harbouring PDZ domains. Here we show that this motif allows E6 interaction with Sorting Nexin 27 (SNX27, an essential component of endosomal recycling pathways. This interaction is highly conserved across E6 proteins from multiple high-risk HPV types and is mediated by a classical PBM-PDZ interaction but unlike many E6 targets, SNX27 is not targeted for degradation by E6. Rather, in HPV-18 positive cell lines the association of SNX27 with components of the retromer complex and the endocytic transport machinery is altered in an E6 PBM-dependent manner. Analysis of a SNX27 cargo, the glucose transporter GLUT1, reveals an E6-dependent maintenance of GLUT1 expression and alteration in its association with components of the endocytic transport machinery. Furthermore, knockdown of E6 in HPV-18 positive cervical cancer cells phenocopies the loss of SNX27, both in terms of GLUT1 expression levels and its vesicular localization, with a concomitant marked reduction in glucose uptake, whilst loss of SNX27 results in slower cell proliferation in low nutrient conditions. These results demonstrate that E6 interaction with SNX27 can alter the recycling of cargo molecules, one consequence of which is modulation of nutrient availability in HPV transformed tumour cells.

  8. APC Inhibits Ligand-Independent Wnt Signaling by the Clathrin Endocytic Pathway.

    Science.gov (United States)

    Saito-Diaz, Kenyi; Benchabane, Hassina; Tiwari, Ajit; Tian, Ai; Li, Bin; Thompson, Joshua J; Hyde, Annastasia S; Sawyer, Leah M; Jodoin, Jeanne N; Santos, Eduardo; Lee, Laura A; Coffey, Robert J; Beauchamp, R Daniel; Williams, Christopher S; Kenworthy, Anne K; Robbins, David J; Ahmed, Yashi; Lee, Ethan

    2018-03-12

    Adenomatous polyposis coli (APC) mutations cause Wnt pathway activation in human cancers. Current models for APC action emphasize its role in promoting β-catenin degradation downstream of Wnt receptors. Unexpectedly, we find that blocking Wnt receptor activity in APC-deficient cells inhibits Wnt signaling independently of Wnt ligand. We also show that inducible loss of APC is rapidly followed by Wnt receptor activation and increased β-catenin levels. In contrast, APC2 loss does not promote receptor activation. We show that APC exists in a complex with clathrin and that Wnt pathway activation in APC-deficient cells requires clathrin-mediated endocytosis. Finally, we demonstrate conservation of this mechanism in Drosophila intestinal stem cells. We propose a model in which APC and APC2 function to promote β-catenin degradation, and APC also acts as a molecular "gatekeeper" to block receptor activation via the clathrin pathway. Copyright © 2018 Elsevier Inc. All rights reserved.

  9. Robust and Adaptive Block Tracking Method Based on Particle Filter

    Directory of Open Access Journals (Sweden)

    Bin Sun

    2015-10-01

    Full Text Available In the field of video analysis and processing, object tracking is attracting more and more attention especially in traffic management, digital surveillance and so on. However problems such as objects’ abrupt motion, occlusion and complex target structures would bring difficulties to academic study and engineering application. In this paper, a fragmentsbased tracking method using the block relationship coefficient is proposed. In this method, we use particle filter algorithm and object region is divided into blocks initially. The contribution of this method is that object features are not extracted just from a single block, the relationship between current block and its neighbor blocks are extracted to describe the variation of the block. Each block is weighted according to the block relationship coefficient when the block is voted on the most matched region in next frame. This method can make full use of the relationship between blocks. The experimental results demonstrate that our method can provide good performance in condition of occlusion and abrupt posture variation.

  10. European Traffic Connections and Croatia

    Directory of Open Access Journals (Sweden)

    Ivan Vuković

    2012-10-01

    Full Text Available The overall traffic connections within Europe are particularlyimportant from the economic point of view of eve1y Europeancountry. Therefore, the development of traffic and trafficinfrastructure in the European Union are considered with specialattention. The main aim of traffic development in the EuropeanUnion is full possible freedom of movement both fromthe technical and organisational aspect, with continuous improvementin traffic safety and environmental protection.Traffic development and connections are one of the essentieddevelopment factors of eve1y modem counlly. Every COU/1-try tends to organise traffic system in the way that suits her best.The same holds for the European Union as well. The leadingbodies in the European Union agree that the EU traffic systemneeds to be set upon such fundamentals that it can follow furtherdevelopment of the community. EU connects 15 membercountries into a single community, and it also plans soon to expandonto new members.The aims set by the Maastricht Agreement have been realisedby announcing the traffic guidelines on infrastructure projectsthat should be realised. In the Commission's proposal tothe Council and the Parliament in 1994, on the development ofTrans-European traffic networks, a ve1y efficient plan was presentedof connecting national traffic networks into a singleTrans-European network, to be realised until 2010.Overall economic and political connections of the EuropeanUnion and the countries in transition are becoming strongereve1y day. Several countries in transition are already preparingfor joining the European Union. This brings to the fore theneed for better traffic connections of EU and the countries intransition.In some sections the adopted Crete corridors pass alsothrough Croatia. Moreover, Croatia is located on the main Europeantraffic routes from the North to the South, and from theEast to the West.

  11. Reports on internet traffic statistics

    NARCIS (Netherlands)

    Hoogesteger, Martijn; de Oliveira Schmidt, R.; Sperotto, Anna; Pras, Aiko

    2013-01-01

    Internet traffic statistics can provide valuable information to network analysts and researchers about the way nowadays networks are used. In the past, such information was provided by Internet2 in a public website called Internet2 NetFlow: Weekly Reports. The website reported traffic statistics

  12. Probabilistic description of traffic flow

    International Nuclear Information System (INIS)

    Mahnke, R.; Kaupuzs, J.; Lubashevsky, I.

    2005-01-01

    A stochastic description of traffic flow, called probabilistic traffic flow theory, is developed. The general master equation is applied to relatively simple models to describe the formation and dissolution of traffic congestions. Our approach is mainly based on spatially homogeneous systems like periodically closed circular rings without on- and off-ramps. We consider a stochastic one-step process of growth or shrinkage of a car cluster (jam). As generalization we discuss the coexistence of several car clusters of different sizes. The basic problem is to find a physically motivated ansatz for the transition rates of the attachment and detachment of individual cars to a car cluster consistent with the empirical observations in real traffic. The emphasis is put on the analogy with first-order phase transitions and nucleation phenomena in physical systems like supersaturated vapour. The results are summarized in the flux-density relation, the so-called fundamental diagram of traffic flow, and compared with empirical data. Different regimes of traffic flow are discussed: free flow, congested mode as stop-and-go regime, and heavy viscous traffic. The traffic breakdown is studied based on the master equation as well as the Fokker-Planck approximation to calculate mean first passage times or escape rates. Generalizations are developed to allow for on-ramp effects. The calculated flux-density relation and characteristic breakdown times coincide with empirical data measured on highways. Finally, a brief summary of the stochastic cellular automata approach is given

  13. Steering Kids to Traffic Safety.

    Science.gov (United States)

    PTA Today, 1991

    1991-01-01

    Guidelines to help parents explain traffic safety to children cover the following: school bus safety (e.g., remain seated, do not shout); walking (e.g., obey traffic signals, cross at crosswalks); driving (e.g., wear seatbelts, enter and exit from the curb side); and biking (e.g., wear helmets, do not ride at night). (SM)

  14. Road traffic noise and stroke

    DEFF Research Database (Denmark)

    Sørensen, Mette; Hvidberg, Martin; Andersen, Zorana J

    2011-01-01

    Epidemiological studies suggest that long-term exposure to road traffic noise increases the risk of cardiovascular disorders. The aim of this study was to investigate the relation between exposure to road traffic noise and risk for stroke, which has not been studied before....

  15. Web traffic and firm performance

    DEFF Research Database (Denmark)

    Farooq, Omar; Aguenaou, Samir

    2013-01-01

    -traffic on firm performance in civil law countries, firms with concentrated ownership, and firms with more intangible assets. All of these groups are characterized by higher agency problems. Our results, therefore, indicate that web-traffic can play a substitute for traditional governance mechanisms in the MENA...

  16. Blocked Randomization with Randomly Selected Block Sizes

    Directory of Open Access Journals (Sweden)

    Jimmy Efird

    2010-12-01

    Full Text Available When planning a randomized clinical trial, careful consideration must be given to how participants are selected for various arms of a study. Selection and accidental bias may occur when participants are not assigned to study groups with equal probability. A simple random allocation scheme is a process by which each participant has equal likelihood of being assigned to treatment versus referent groups. However, by chance an unequal number of individuals may be assigned to each arm of the study and thus decrease the power to detect statistically significant differences between groups. Block randomization is a commonly used technique in clinical trial design to reduce bias and achieve balance in the allocation of participants to treatment arms, especially when the sample size is small. This method increases the probability that each arm will contain an equal number of individuals by sequencing participant assignments by block. Yet still, the allocation process may be predictable, for example, when the investigator is not blind and the block size is fixed. This paper provides an overview of blocked randomization and illustrates how to avoid selection bias by using random block sizes.

  17. Blocked randomization with randomly selected block sizes.

    Science.gov (United States)

    Efird, Jimmy

    2011-01-01

    When planning a randomized clinical trial, careful consideration must be given to how participants are selected for various arms of a study. Selection and accidental bias may occur when participants are not assigned to study groups with equal probability. A simple random allocation scheme is a process by which each participant has equal likelihood of being assigned to treatment versus referent groups. However, by chance an unequal number of individuals may be assigned to each arm of the study and thus decrease the power to detect statistically significant differences between groups. Block randomization is a commonly used technique in clinical trial design to reduce bias and achieve balance in the allocation of participants to treatment arms, especially when the sample size is small. This method increases the probability that each arm will contain an equal number of individuals by sequencing participant assignments by block. Yet still, the allocation process may be predictable, for example, when the investigator is not blind and the block size is fixed. This paper provides an overview of blocked randomization and illustrates how to avoid selection bias by using random block sizes.

  18. 31 CFR 595.301 - Blocked account; blocked property.

    Science.gov (United States)

    2010-07-01

    ... (Continued) OFFICE OF FOREIGN ASSETS CONTROL, DEPARTMENT OF THE TREASURY TERRORISM SANCTIONS REGULATIONS General Definitions § 595.301 Blocked account; blocked property. The terms blocked account and blocked...

  19. Dissociative Tendencies and Traffic Incidents

    Directory of Open Access Journals (Sweden)

    Valle, Virginia

    2012-01-01

    Full Text Available This paper analyses the relationship between dissociative experiences and road traffic incidents (crashes and traffic tickets in drivers (n=295 from Mar del Plata (Argentina city. A self-report questionnaire was applied to assess traffic crash involvement and sociodemographic variables. Dissociative tendencies were assessed by a modified version of the DES scale. To examine differences in DES scores tests of the difference of means were applied. Drivers who reported to be previously involved in traffic incidents obtained higher puntuations in the dissociative experiences scale than drivers who did not report such events. This result is observed for the total scale and for the three sub-scales (absorption, amnesia and depersonalization. However, differences appeared mainly for minor damage collisions. Further studies are needed to evaluate the role of dissociative tendencies as a risk factor in road traffic safety.

  20. Gas Kinetics of Traffic Jam

    Science.gov (United States)

    Nagatani, Takashi

    1997-04-01

    The kinetics of one-dimensional traffic flow is descibed in terms of Boltzmann-like gas kinetic equation. Paveri-Fontana's gas kinetic equation is modified to take into account the desired velocity depending on the car density. A discrete version of the gas kinetic equation is derived to numerically solve the equation. The velocity distributions are calculated by a numerical method. It is found that the traffic jam is formed in the congested traffic flow when the car density is higher than the critical value. The traffic jam propagates backward, its propagation velocity increases with the accerelation and the density within the jam decreases with increasing accerelation. It is shown that the velocity distributions change significantly before and after the traffic jam.

  1. Model for traffic emissions estimation

    Science.gov (United States)

    Alexopoulos, A.; Assimacopoulos, D.; Mitsoulis, E.

    A model is developed for the spatial and temporal evaluation of traffic emissions in metropolitan areas based on sparse measurements. All traffic data available are fully employed and the pollutant emissions are determined with the highest precision possible. The main roads are regarded as line sources of constant traffic parameters in the time interval considered. The method is flexible and allows for the estimation of distributed small traffic sources (non-line/area sources). The emissions from the latter are assumed to be proportional to the local population density as well as to the traffic density leading to local main arteries. The contribution of moving vehicles to air pollution in the Greater Athens Area for the period 1986-1988 is analyzed using the proposed model. Emissions and other related parameters are evaluated. Emissions from area sources were found to have a noticeable share of the overall air pollution.

  2. The NASA Air Traffic Management Ontology (atmonto)

    Data.gov (United States)

    National Aeronautics and Space Administration — The NASA ATM (Air Traffic Management) Ontology describes classes, properties, and relationships relevant to the domain of air traffic management, and represents...

  3. Traffic information computing platform for big data

    Science.gov (United States)

    Duan, Zongtao; Li, Ying; Zheng, Xibin; Liu, Yan; Dai, Jiting; Kang, Jun

    2014-10-01

    Big data environment create data conditions for improving the quality of traffic information service. The target of this article is to construct a traffic information computing platform for big data environment. Through in-depth analysis the connotation and technology characteristics of big data and traffic information service, a distributed traffic atomic information computing platform architecture is proposed. Under the big data environment, this type of traffic atomic information computing architecture helps to guarantee the traffic safety and efficient operation, more intelligent and personalized traffic information service can be used for the traffic information users.

  4. Traffic information computing platform for big data

    International Nuclear Information System (INIS)

    Duan, Zongtao; Li, Ying; Zheng, Xibin; Liu, Yan; Dai, Jiting; Kang, Jun

    2014-01-01

    Big data environment create data conditions for improving the quality of traffic information service. The target of this article is to construct a traffic information computing platform for big data environment. Through in-depth analysis the connotation and technology characteristics of big data and traffic information service, a distributed traffic atomic information computing platform architecture is proposed. Under the big data environment, this type of traffic atomic information computing architecture helps to guarantee the traffic safety and efficient operation, more intelligent and personalized traffic information service can be used for the traffic information users

  5. Automated mixed traffic vehicle design AMTV 2

    Science.gov (United States)

    Johnston, A. R.; Marks, R. A.; Cassell, P. L.

    1982-01-01

    The design of an improved and enclosed Automated Mixed Traffic Transit (AMTT) vehicle is described. AMTT is an innovative concept for low-speed tram-type transit in which suitable vehicles are equipped with sensors and controls to permit them to operate in an automated mode on existing road or walkway surfaces. The vehicle chassis and body design are presented in terms of sketches and photographs. The functional design of the sensing and control system is presented, and modifications which could be made to the baseline design for improved performance, in particular to incorporate a 20-mph capability, are also discussed. The vehicle system is described at the block-diagram-level of detail. Specifications and parameter values are given where available.

  6. Massive calcium-activated endocytosis without involvement of classical endocytic proteins.

    Science.gov (United States)

    Lariccia, Vincenzo; Fine, Michael; Magi, Simona; Lin, Mei-Jung; Yaradanakul, Alp; Llaguno, Marc C; Hilgemann, Donald W

    2011-01-01

    We describe rapid massive endocytosis (MEND) of >50% of the plasmalemma in baby hamster kidney (BHK) and HEK293 cells in response to large Ca transients. Constitutively expressed Na/Ca exchangers (NCX1) are used to generate Ca transients, whereas capacitance recording and a membrane tracer dye, FM 4-64, are used to monitor endocytosis. With high cytoplasmic adenosine triphosphate (ATP; >5 mM), Ca influx causes exocytosis followed by MEND. Without ATP, Ca transients cause only exocytosis. MEND can then be initiated by pipette perfusion of ATP, and multiple results indicate that ATP acts via phosphatidylinositol-bis 4,5-phosphate (PIP(2)) synthesis: PIP(2) substitutes for ATP to induce MEND. ATP-activated MEND is blocked by an inositol 5-phosphatase and by guanosine 5'-[γ-thio]triphosphate (GTPγS). Block by GTPγS is overcome by the phospholipase C inhibitor, U73122, and PIP(2) induces MEND in the presence of GTPγS. MEND can occur in the absence of ATP and PIP(2) when cytoplasmic free Ca is clamped to 10 µM or more by Ca-buffered solutions. ATP-independent MEND occurs within seconds during Ca transients when cytoplasmic solutions contain polyamines (e.g., spermidine) or the membrane is enriched in cholesterol. Although PIP(2) and cholesterol can induce MEND minutes after Ca transients have subsided, polyamines must be present during Ca transients. MEND can reverse over minutes in an ATP-dependent fashion. It is blocked by brief β-methylcyclodextrin treatments, and tests for involvement of clathrin, dynamins, calcineurin, and actin cytoskeleton were negative. Therefore, we turned to the roles of lipids. Bacterial sphingomyelinases (SMases) cause similar MEND responses within seconds, suggesting that ceramide may be important. However, Ca-activated MEND is not blocked by reagents that inhibit SMases. MEND is abolished by the alkylating phospholipase A(2) inhibitor, bromoenol lactone, whereas exocytosis remains robust, and Ca influx causes MEND in cardiac myocytes

  7. Generalized Block Failure

    DEFF Research Database (Denmark)

    Jönsson, Jeppe

    2015-01-01

    Block tearing is considered in several codes as a pure block tension or a pure block shear failure mechanism. However in many situations the load acts eccentrically and involves the transfer of a substantial moment in combination with the shear force and perhaps a normal force. A literature study...... shows that no readily available tests with a well-defined substantial eccentricity have been performed. This paper presents theoretical and experimental work leading towards generalized block failure capacity methods. Simple combination of normal force, shear force and moment stress distributions along...

  8. Blocking-resistant communication through domain fronting

    Directory of Open Access Journals (Sweden)

    Fifield David

    2015-06-01

    Full Text Available We describe “domain fronting,” a versatile censorship circumvention technique that hides the remote endpoint of a communication. Domain fronting works at the application layer, using HTTPS, to communicate with a forbidden host while appearing to communicate with some other host, permitted by the censor. The key idea is the use of different domain names at different layers of communication. One domain appears on the “outside” of an HTTPS request—in the DNS request and TLS Server Name Indication—while another domain appears on the “inside”—in the HTTP Host header, invisible to the censor under HTTPS encryption. A censor, unable to distinguish fronted and nonfronted traffic to a domain, must choose between allowing circumvention traffic and blocking the domain entirely, which results in expensive collateral damage. Domain fronting is easy to deploy and use and does not require special cooperation by network intermediaries. We identify a number of hard-to-block web services, such as content delivery networks, that support domain-fronted connections and are useful for censorship circumvention. Domain fronting, in various forms, is now a circumvention workhorse. We describe several months of deployment experience in the Tor, Lantern, and Psiphon circumvention systems, whose domain-fronting transports now connect thousands of users daily and transfer many terabytes per month.

  9. A Survey on Urban Traffic Management System Using Wireless Sensor Networks

    Directory of Open Access Journals (Sweden)

    Kapileswar Nellore

    2016-01-01

    Full Text Available Nowadays, the number of vehicles has increased exponentially, but the bedrock capacities of roads and transportation systems have not developed in an equivalent way to efficiently cope with the number of vehicles traveling on them. Due to this, road jamming and traffic correlated pollution have increased with the associated adverse societal and financial effect on different markets worldwide. A static control system may block emergency vehicles due to traffic jams. Wireless Sensor networks (WSNs have gained increasing attention in traffic detection and avoiding road congestion. WSNs are very trendy due to their faster transfer of information, easy installation, less maintenance, compactness and for being less expensive compared to other network options. There has been significant research on Traffic Management Systems using WSNs to avoid congestion, ensure priority for emergency vehicles and cut the Average Waiting Time (AWT of vehicles at intersections. In recent decades, researchers have started to monitor real-time traffic using WSNs, RFIDs, ZigBee, VANETs, Bluetooth devices, cameras and infrared signals. This paper presents a survey of current urban traffic management schemes for priority-based signalling, and reducing congestion and the AWT of vehicles. The main objective of this survey is to provide a taxonomy of different traffic management schemes used for avoiding congestion. Existing urban traffic management schemes for the avoidance of congestion and providing priority to emergency vehicles are considered and set the foundation for further research.

  10. Framework for Traffic Congestion Management

    Directory of Open Access Journals (Sweden)

    Mahmud Hassan TALUKDAR

    2013-06-01

    Full Text Available Traffic Congestion is one of many serious global problems in all great cities resulted from rapid urbanization which always exert negative externalities upon society. The solution of traffic congestion is highly geocentric and due to its heterogeneous nature, curbing congestion is one of the hard tasks for transport planners. It is not possible to suggest unique traffic congestion management framework which could be absolutely applied for every great cities. Conversely, it is quite feasible to develop a framework which could be used with or without minor adjustment to deal with congestion problem. So, the main aim of this paper is to prepare a traffic congestion mitigation framework which will be useful for urban planners, transport planners, civil engineers, transport policy makers, congestion management researchers who are directly or indirectly involved or willing to involve in the task of traffic congestion management. Literature review is the main source of information of this study. In this paper, firstly, traffic congestion is defined on the theoretical point of view and then the causes of traffic congestion are briefly described. After describing the causes, common management measures, using world- wide, are described and framework for supply side and demand side congestion management measures are prepared.

  11. Spreading of Traffic Jam in a Traffic Flow Model

    Science.gov (United States)

    Nagatani, Takashi

    1993-04-01

    A cellular automaton (CA) model is presented to simulate the traffic jam induced by a traffic accident. The spreading of jamming cars induced by a car crash is investigated by computer simulation. An analogy is proposed between the crystal growth and the traffic-jam spreading. The scaling behavior of the traffic-jam spreading is studied. It is shown that the number N of jamming cars scales as N≈t2.34± 0.03 for p above the dynamical jamming transition pc{=}0.35 and N≈t1.07 below pc where t is the time and p is the density of cars. The time constant ts, which is the time required for all cars to stop, scales as ts≈p-1.07± 0.03 for p

  12. Traffic jams: dynamics and control.

    Science.gov (United States)

    Orosz, Gábor; Wilson, R Eddie; Stépán, Gábor

    2010-10-13

    This introductory paper reviews the current state-of-the-art scientific methods used for modelling, analysing and controlling the dynamics of vehicular traffic. Possible mechanisms underlying traffic jam formation and propagation are presented from a dynamical viewpoint. Stable and unstable motions are described that may give the skeleton of traffic dynamics, and the effects of driver behaviour are emphasized in determining the emergent state in a vehicular system. At appropriate points, references are provided to the papers published in the corresponding Theme Issue.

  13. OPTIMASI BANDWITH MENGGUNAKAN TRAFFIC SHAPPING

    Directory of Open Access Journals (Sweden)

    Imam Riadi

    2012-05-01

    Full Text Available Perkembangan layanan komunikasi telah berkembang sangat pesat. Salah satunya adalah pemanfaatan penggunaan bandwith untuk mengakases jaringan Internet. Traffic shapping bandwidth dapat memberikan efesiensi dalam hal pemanfaatan bandwidth pada instansi yang melakukan manajemen dalam lalulintas jaringannya. Metode yang digunakan dalam penelitian ini adalah metode literatur yaitu metode pengumpulan data yang dilakukan dengan mengumpulkan sumber-sumber data yang terkait dan metode eksperimen yaitu melakukan penelitian dengan mengkonfigurasi Mikrotik RouterOS untuk melakukan traffic shapping bandwidth. Hasil penelitian ini adalah router yang dapat melakukan traffic shapping sehingga kualitas koneksi menjadi lebih baik untuk mengakses dan mengambil data dari sebuah website.

  14. Traffic Light Detection at Night

    DEFF Research Database (Denmark)

    Jensen, Morten Bornø; Philipsen, Mark Philip; Bahnsen, Chris

    2015-01-01

    of three detectors based on heuristic models and one learning-based detector. Evaluation is done on night-time data from the public LISA Traffic Light Dataset. The learning-based detector out- performs the model-based detectors in both precision and recall. The learning-based detector achieves an average......Traffic light recognition (TLR) is an integral part of any in- telligent vehicle, it must function both at day and at night. However, the majority of TLR research is focused on day-time scenarios. In this paper we will focus on detection of traffic lights at night and evalu- ate the performance...

  15. Eps15 is recruited to the plasma membrane upon epidermal growth factor receptor activation and localizes to components of the endocytic pathway during receptor internalization

    DEFF Research Database (Denmark)

    Torrisi, M R; Lotti, L V; Belleudi, F

    1999-01-01

    role for eps15 in receptor-mediated endocytosis. In this study we show that, upon activation of the EGFR kinase, eps15 undergoes dramatic relocalization consisting of 1) initial relocalization to the plasma membrane and 2) subsequent colocalization with the EGFR in various intracellular compartments......Eps15 is a substrate for the tyrosine kinase of the epidermal growth factor receptor (EGFR) and is characterized by the presence of a novel protein:protein interaction domain, the EH domain. Eps15 also stably binds the clathrin adaptor protein complex AP-2. Previous work demonstrated an essential......, suggesting sustained phosphorylation in endocytic compartments. Our results are consistent with a model in which eps15 undergoes cycles of association:dissociation with membranes and suggest multiple roles for this protein in the endocytic pathway....

  16. [Comics for traffic education: evaluation of a traffic safety campaign].

    Science.gov (United States)

    Bonfadelli, H

    1989-01-01

    Traffic safety campaigns often are ineffective to change driving behavior because they don't reach the target group or are recognized only by people who are already interested or concerned. The evaluation of a traffic safety campaign called "Leo Lässig", addressed to young new drivers, shows that recognition and acceptance by the target group were stimulated by the age-conform means of comic-strips.

  17. Homogeneous bilateral block shifts

    Indian Academy of Sciences (India)

    Homogeneous bilateral block shifts. ADAM KORÁNYI. Department of Mathematics, The Graduate Center, City University of New York,. New York, NY 10016, USA. E-mail: Adam.Koranyi@lehman.cuny.edu. MS received 18 January 2013. Abstract. A new 3-parameter family of homogeneous 2-by-2 block shifts is described.

  18. Related Drupal Nodes Block

    NARCIS (Netherlands)

    Van der Vegt, Wim

    2010-01-01

    Related Drupal Nodes Block This module exposes a block that uses Latent Semantic Analysis (Lsa) internally to suggest three nodes that are relevant to the node a user is viewing. This module performs three tasks. 1) It periodically indexes a Drupal site and generates a Lsa Term Document Matrix.

  19. Direct Visualization of Ebola Virus Fusion Triggering in the Endocytic Pathway

    Directory of Open Access Journals (Sweden)

    Jennifer S. Spence

    2016-02-01

    Full Text Available Ebola virus (EBOV makes extensive and intricate use of host factors in the cellular endosomal/lysosomal pathway to release its genome into the cytoplasm and initiate infection. Following viral internalization into endosomes, host cysteine proteases cleave the EBOV fusion glycoprotein (GP to unmask the binding site for its intracellular receptor, the cholesterol transporter Niemann-Pick C1 (NPC1. GP-NPC1 interaction is required for viral entry. Despite these and other recent discoveries, late events in EBOV entry following GP-NPC1 binding and culminating in GP-catalyzed fusion between viral and cellular lipid bilayers remain enigmatic. A mechanistic understanding of EBOV membrane fusion has been hampered by the failure of previous efforts to reconstitute fusion in vitro or at the cell surface. This report describes an assay to monitor initial steps directly in EBOV membrane fusion—triggering of GP and virus-cell lipid mixing—by single virions in live cells. Fusogenic triggering of GP occurs predominantly in Rab7-positive (Rab7+ endosomes, absolutely requires interaction between proteolytically primed GP and NPC1, and is blocked by key GP-specific neutralizing antibodies with therapeutic potential. Unexpectedly, cysteine protease inhibitors do not inhibit lipid mixing by virions bearing precleaved GP, even though they completely block cytoplasmic entry by these viruses, as shown previously. These results point to distinct cellular requirements for different steps in EBOV membrane fusion and suggest a model in which host cysteine proteases are dispensable for GP fusion triggering after NPC1 binding but are required for the formation of fusion pores that permit genome delivery.

  20. Control rod blocking monitor

    International Nuclear Information System (INIS)

    Suzuki, Shigeru.

    1993-01-01

    The number of times for setting up a control rod blocking monitor of a BWR type power plant is remarkably reduced to mitigate operator's burden. In the control rod blocking monitor, trip levels, as a judging standard upon outputting control rod blocking inhibition signals, are set up stepwise depending on the power level around control rods put to blocking control. The present invention comprises an allowance judging means capable of setting up trip levels for each of power levels corresponding to a plurality of control rods at once if the power levels are within the set up allowable range. With such a constitution, the set up allowable range is determined previously in the allowance judging means. Accordingly, when a gang blocking is conducted to control rods, if power levels around the control rods are increased at once into the set up allowable range, the trip levels for each of the control rods are set up at once. (I.S.)

  1. 49 CFR 1139.2 - Traffic study.

    Science.gov (United States)

    2010-10-01

    ... “base-calendar year—actual.” The study shall include a probability sampling of the actual traffic... 49 Transportation 8 2010-10-01 2010-10-01 false Traffic study. 1139.2 Section 1139.2... of General Commodities § 1139.2 Traffic study. (a) The respondents shall submit a traffic study for...

  2. Real-Time Traffic Signal Control for Optimization of Traffic Jam Probability

    Science.gov (United States)

    Cui, Cheng-You; Shin, Ji-Sun; Miyazaki, Michio; Lee, Hee-Hyol

    Real-time traffic signal control is an integral part of urban traffic control system. It can control traffic signals online according to variation of traffic flow. In this paper, we propose a new method for the real-time traffic signal control system. The system uses a Cellular Automaton model and a Bayesian Network model to predict probabilistic distributions of standing vehicles, and uses a Particle Swarm Optimization method to calculate optimal traffic signals. A simulation based on real traffic data was carried out to show the effectiveness of the proposed real-time traffic signal control system CAPSOBN using a micro traffic simulator.

  3. Development of Deflection Prediction Model for Concrete Block Pavement Considering the Block Shapes and Construction Patterns

    Directory of Open Access Journals (Sweden)

    Wuguang Lin

    2016-01-01

    Full Text Available Concrete block pavement (CBP is distinct from typical concrete or asphalt pavements. It is built by using individual blocks with unique construction patterns forming a discrete surface layer to bear traffic loadings. The surface structure of CBP varies depending on the block shapes and construction patterns, so it is hard to apply a general equivalent elastic modulus estimation method to define the surface structural strength. In this study, FEM analysis and dynamic loading test were carried out to develop a deflection prediction model for CBP considering the block shapes and construction patterns. Based on the analysis results, it was found that block shapes did not have much effect on load distribution, whereas construction patterns did. By applying the deflection prediction model to the rutting model for CBP proposed by Sun, the herringbone bond pattern showed the best performance comparing with stretcher bond or basket weave bond pattern. As the load repetition increased to 1.2 million, the rutting depth of CBP constructed by herringbone bond pattern was 2 mm smaller than those constructed by the other two patterns.

  4. Characterization and Modeling of Network Traffic

    DEFF Research Database (Denmark)

    Shawky, Ahmed; Bergheim, Hans; Ragnarsson, Olafur

    2011-01-01

    This paper attempts to characterize and model backbone network traffic, using a small number of statistics. In order to reduce cost and processing power associated with traffic analysis. The parameters affecting the behaviour of network traffic are investigated and the choice is that inter......-arrival time, IP addresses, port numbers and transport protocol are the only necessary parameters to model network traffic behaviour. In order to recreate this behaviour, a complex model is needed which is able to recreate traffic behaviour based on a set of statistics calculated from the parameters values....... The model investigates the traffic generation mechanisms, and grouping traffic into flows and applications....

  5. Automatic Traffic Recorder (ATR) Stations

    Data.gov (United States)

    Department of Homeland Security — The data included in the GIS Traffic Stations Version database have been assimilated from station description files provided by FHWA for Weigh-in-Motion (WIM), and...

  6. Traffic safety in the future

    OpenAIRE

    EKKEHARD, Brühning; OKAMURA, Kazuko; FLEURY, Dominique; BRILON, Werner; DINGES, David F.; ABE, Takashi

    2013-01-01

    This booklet is divided in 4 parts : Traffic Safety in Japan and Germany - Success, Deficiencies, Future Potentials. Research on the Territorial Approach to Safety. Reliability of Motorway Operation. Transportation Safety and Operator Sleepiness: Where Biology Needs Technology.

  7. Traffic fatalities and economic growth

    Science.gov (United States)

    2003-04-01

    As countries develop death rates usually fall, especially for diseases that affect the young and result in substantial life-years lost. Deaths due to traffic accidents are a notable exception: the growth in motor vehicles that accompanies economic gr...

  8. Traffic behavior at freeway bottlenecks.

    Science.gov (United States)

    2014-09-01

    This study examines traffic behavior in the vicinity of a freeway bottleneck, revisiting commonly held : assumptions and uncovering systematic biases that likely have distorted empirical studies of bottleneck : formation, capacity drop, and the funda...

  9. Assessment of Traffic Noise Impacts

    DEFF Research Database (Denmark)

    Rich, Jeppe Husted; Nielsen, Otto Anker

    2004-01-01

    A steady growth in traffic intensities in most urban areas throughout the world has forced planners and politicians to seriously consider the resulting environmental impact, such as traffic noise, accidents and air pollution. The assessment of such negative factors is needed in order to reveal...... the true social benefit of infrastructure plans. The paper presents a noise assessment model for the Copenhagen region, which brings together GIS technology and non-linear hedonic regression models to reveal the implicit costs of traffic noise measured as the marginal percentage loss in property values...... with respect to the decibel traffic noise. The model distinguishes between houses and apartments and shows that the ability to include refined accessibility variables have significant impact on estimated prices....

  10. Estimating Emissions from Railway Traffic

    DEFF Research Database (Denmark)

    Jørgensen, Morten W.; Sorenson, Spencer C.

    1998-01-01

    Several parameters of importance for estimating emissions from railway traffic are discussed, and typical results presented. Typical emissions factors from diesel engines and electrical power generation are presented, and the effect of differences in national electrical generation sources illustr...

  11. Game theory and traffic assignment.

    Science.gov (United States)

    2013-09-01

    Traffic assignment is used to determine the number of users on roadway links in a network. While this problem has : been widely studied in transportation literature, its use of the concept of equilibrium has attracted considerable interest : in the f...

  12. Stochastic control of traffic patterns

    DEFF Research Database (Denmark)

    Gaididei, Yuri B.; Gorria, Carlos; Berkemer, Rainer

    2013-01-01

    A stochastic modulation of the safety distance can reduce traffic jams. It is found that the effect of random modulation on congestive flow formation depends on the spatial correlation of the noise. Jam creation is suppressed for highly correlated noise. The results demonstrate the advantage...... of heterogeneous performance of the drivers in time as well as individually. This opens the possibility for the construction of technical tools to control traffic jam formation....

  13. Estimating emissions from railway traffic

    Energy Technology Data Exchange (ETDEWEB)

    Joergensen, M.W.; Sorenson, C.

    1997-07-01

    The report discusses methods that can be used to estimate the emissions from various kinds of railway traffic. The methods are based on the estimation of the energy consumption of the train, so that comparisons can be made between electric and diesel driven trains. Typical values are given for the necessary traffic parameters, emission factors, and train loading. Detailed models for train energy consumption are presented, as well as empirically based methods using average train speed and distance between stop. (au)

  14. Future Emissions from Railway Traffic

    DEFF Research Database (Denmark)

    Sorenson, Spencer C.

    1998-01-01

    In investigation of the expected development in factors which influence railway energy consumption and emissions. Traffic factors such as train speed, load, an occupancy were considered. Tehcnical factors such an emissions factors, fleet composition and train weight were also considered. An estim......In investigation of the expected development in factors which influence railway energy consumption and emissions. Traffic factors such as train speed, load, an occupancy were considered. Tehcnical factors such an emissions factors, fleet composition and train weight were also considered...

  15. An analysis of the traffic safety phenomenon.

    OpenAIRE

    Asmussen, E. & Kranenburg, A.

    1982-01-01

    The lack of traffic safety is a combination of the critical coincidence of circumstances in the traffic of incidents (near-accidents) and accidents with unwanted (permanent) consequences, such as fatalities, injured and disabled persons and material damage. This definition covers the whole of the critical coincidence of circumstances in traffic. In order to elucidate the phenomenon of traffic safety or the lack of traffic safety, accidents and incidents can be analysed.

  16. Economic Efficacy of Road Traffic Safety Measures

    OpenAIRE

    Gul, Ejaz

    2013-01-01

    The number of road traffic casualties is still very lofty and the trend shows a boost with each passing day. The road traffic accidents involve fatalities due to which economic resources are damaged and the productivity of the economy is correspondingly impaired. Costs resulting from traffic accidents represent the largest single part of the overall cost of traffic to the economy. Knowledge about the harm of these traffic accidents to the economy is essential if measures to reduce road traffi...

  17. Economic Evaluation of Road Traffic Safety Measures

    OpenAIRE

    Gul, Ejaz

    2013-01-01

    The number of road traffic casualties is still very lofty and the trend shows a boost with each passing day. The road traffic accidents involve fatalities due to which economic resources are damaged and the productivity of the economy is correspondingly impaired. Costs resulting from traffic accidents represent the largest single part of the overall cost of traffic to the economy. Knowledge about the harm of these traffic accidents to the economy is essential if measures to reduce road traffi...

  18. Simulation Model of Traffic Jam at Crossroads

    OpenAIRE

    Mladen Kalajžić; Katica Miloš; Mirela Muić

    2002-01-01

    Traffic congestion is one of the major problems in most cities.It is the consequence of unavoidable motorization, butalso, in many cases, of improper solutions considering constructionof roads or organisation of traffic.This paper deals with one problematic crossroad in thetown of Zadar in which traffic jams occur due to poor organisationof traffic. Using mathematical simulation, the first partproves that traffic jams will certainly occur, and in the secondpart, crossroads signalling is consi...

  19. Regulatory measures for traffic safety

    International Nuclear Information System (INIS)

    Veerapur, R.D.; Bharambe, S.D.; Patnaik, S.K.; Tandle, A.K.; Sonawane, K.A.; Kumar, Rajesh; Venkat Subramanian, K.

    2017-01-01

    Traffic safety is an issue related to occupational safety not restricted alone to the transportation but extends beyond. BARC has many facilities spread across large area in Mumbai and outside Mumbai. BARC deploys large number of buses, mini buses, jeeps and cars for commuting its employees to reach BARC and for commuting within BARC premises. Additionally, trucks, fire tenders, trailers etc. are also deployed for transportation of materials. No moving vehicle is ever free of the possibility of involvement in an accident. Vehicular accidents and the fatalities on road are the result of inter-play of a number of factors. The vehicle population has been steadily increasing with the pace picking up significantly in recent past. Increase in vehicle population in the face of limited road space used by a large variety of traffic has heightened the need and urgency for a well-thought-out road safety. Therefore, existence of regulatory authority to regulate traffic and vehicles to ensure safety of its employees and vehicles is very essential. BARC Traffic Safety Committee (BTSC), which is the regulating body for traffic safety is responsible for ensuring overall traffic safety. (author)

  20. Predictability of blocking

    International Nuclear Information System (INIS)

    Tosi, E.; Ruti, P.; Tibaldi, S.; D'Andrea, F.

    1994-01-01

    Tibaldi and Molteni (1990, hereafter referred to as TM) had previously investigated operational blocking predictability by the ECMWF model and the possible relationships between model systematic error and blocking in the winter season of the Northern Hemisphere, using seven years of ECMWF operational archives of analyses and day 1 to 10 forecasts. They showed that fewer blocking episodes than in the real atmosphere were generally simulated by the model, and that this deficiency increased with increasing forecast time. As a consequence of this, a major contribution to the systematic error in the winter season was shown to derive from the inability of the model to properly forecast blocking. In this study, the analysis performed in TM for the first seven winter seasons of the ECMWF operational model is extended to the subsequent five winters, during which model development, reflecting both resolution increases and parametrisation modifications, continued unabated. In addition the objective blocking index developed by TM has been applied to the observed data to study the natural low frequency variability of blocking. The ability to simulate blocking of some climate models has also been tested

  1. CD163-L1 Is an Endocytic Macrophage Protein Strongly Regulated by Mediators in the Inflammatory Response

    DEFF Research Database (Denmark)

    Moeller, Jesper B; Nielsen, Marianne J; Reichhardt, Martin P

    2012-01-01

    exhibits similarity to CD163 in terms of structure and regulated expression in cultured monocytes but shows clear differences compared with the known CD163 ligand preferences and expression pattern in the pool of tissue macrophages. We postulate that CD163-L1 functions as a scavenger receptor for one......CD163-L1 belongs to the group B scavenger receptor cysteine-rich family of proteins, where the CD163-L1 gene arose by duplication of the gene encoding the hemoglobin scavenger receptor CD163 in late evolution. The current data demonstrate that CD163-L1 is highly expressed and colocalizes with CD163......-phase mediator IL-6 and the anti-inflammatory mediator IL-10 but is suppressed by the proinflammatory mediators IL-4, IL-13, TNF-α, and LPS/IFN-γ. Furthermore, we show that CD163-L1 is an endocytic receptor, which internalizes independently of cross-linking through a clathrin-mediated pathway. Two cytoplasmic...

  2. Endocytic function is critical for influenza A virus infection via DC-SIGN and L-SIGN

    Science.gov (United States)

    Gillespie, Leah; Roosendahl, Paula; Ng, Wy Ching; Brooks, Andrew G.; Reading, Patrick C.; Londrigan, Sarah L.

    2016-01-01

    The ubiquitous presence of cell-surface sialic acid (SIA) has complicated efforts to identify specific transmembrane glycoproteins that function as bone fide entry receptors for influenza A virus (IAV) infection. The C-type lectin receptors (CLRs) DC-SIGN (CD209) and L-SIGN (CD209L) enhance IAV infection however it is not known if they act as attachment factors, passing virions to other unknown receptors for virus entry, or as authentic entry receptors for CLR-mediated virus uptake and infection. Sialic acid-deficient Lec2 Chinese Hamster Ovary (CHO) cell lines were resistant to IAV infection whereas expression of DC-SIGN/L-SIGN restored susceptibility of Lec2 cells to pH- and dynamin-dependent infection. Moreover, Lec2 cells expressing endocytosis-defective DC-SIGN/L-SIGN retained capacity to bind IAV but showed reduced susceptibility to infection. These studies confirm that DC-SIGN and L-SIGN are authentic endocytic receptors for IAV entry and infection. PMID:26763587

  3. Diagnosis diagrams for passing signals on an automatic block signaling railway section

    Science.gov (United States)

    Spunei, E.; Piroi, I.; Chioncel, C. P.; Piroi, F.

    2018-01-01

    This work presents a diagnosis method for railway traffic security installations. More specifically, the authors present a series of diagnosis charts for passing signals on a railway block equipped with an automatic block signaling installation. These charts are based on the exploitation electric schemes, and are subsequently used to develop a diagnosis software package. The thus developed software package contributes substantially to a reduction of failure detection and remedy for these types of installation faults. The use of the software package eliminates making wrong decisions in the fault detection process, decisions that may result in longer remedy times and, sometimes, to railway traffic events.

  4. 31 CFR 594.301 - Blocked account; blocked property.

    Science.gov (United States)

    2010-07-01

    ... (Continued) OFFICE OF FOREIGN ASSETS CONTROL, DEPARTMENT OF THE TREASURY GLOBAL TERRORISM SANCTIONS REGULATIONS General Definitions § 594.301 Blocked account; blocked property. The terms blocked account and...

  5. Symmetry breaking in optimal timing of traffic signals on an idealized two-way street.

    Science.gov (United States)

    Panaggio, Mark J; Ottino-Löffler, Bertand J; Hu, Peiguang; Abrams, Daniel M

    2013-09-01

    Simple physical models based on fluid mechanics have long been used to understand the flow of vehicular traffic on freeways; analytically tractable models of flow on an urban grid, however, have not been as extensively explored. In an ideal world, traffic signals would be timed such that consecutive lights turned green just as vehicles arrived, eliminating the need to stop at each block. Unfortunately, this "green-wave" scenario is generally unworkable due to frustration imposed by competing demands of traffic moving in different directions. Until now this has typically been resolved by numerical simulation and optimization. Here, we develop a theory for the flow in an idealized system consisting of a long two-way road with periodic intersections. We show that optimal signal timing can be understood analytically and that there are counterintuitive asymmetric solutions to this signal coordination problem. We further explore how these theoretical solutions degrade as traffic conditions vary and automotive density increases.

  6. Bundle Branch Block

    Science.gov (United States)

    ... 2015. Bundle branch block Symptoms & causes Diagnosis & treatment Advertisement Mayo Clinic does not endorse companies or products. ... a Job Site Map About This Site Twitter Facebook Google YouTube Pinterest Mayo Clinic is a not- ...

  7. Blocked Urethral Valves

    Science.gov (United States)

    ... the penis. Rarely, small membranes form across the urethra in boys early in pregnancy, and they can block the flow of urine out of the bladder. These membranes are called posterior urethral valves and can have life-threatening consequences ...

  8. Optoelectronics using block copolymers.

    Energy Technology Data Exchange (ETDEWEB)

    Botiz, I.; Darling, S. B.; Center for Nanoscale Materials

    2010-05-01

    Block copolymers, either as semiconductors themselves or as structure directors, are emerging as a promising class of materials for understanding and controlling processes associated with both photovoltaic energy conversion and light emitting devices.

  9. Minimum-block-generated flexible-grouping-based spectrum assignment for flex-grid optical networks

    Science.gov (United States)

    Qiu, Yang; Xu, Jing

    2017-11-01

    We investigate the spectrum fragmentation issue in flex-grid optical networks and propose a minimum-block-generated flexible-grouping-based spectrum assignment algorithm. By dividing the spectrum resources into several flexible groups according to the bandwidth requirements of service requests for their accommodation and minimizing the generated isolated spectrum blocks inside each spectrum group, the proposed algorithm can not only reduce spectrum fragments but also enhance networking performance (e.g. blocking probability) due to the improved spectrum contiguity inside each spectrum group with no traffic disruption or any extra components. The simulation results verify that the proposed algorithm can remarkably reduce spectrum fragments with a low blocking probability.

  10. Real-Time Microscopic Traffic Simulation and Optimization at Intersections with Video Traffic Detection

    OpenAIRE

    Liang, Zijun; Flötteröd, Yun-Pang; Chen, Hong; Sohr, Alexander; Bei, Xiaoxu; Bottazzi, Maximiliano; Trumpold, Jan

    2018-01-01

    In this paper, real-time vehicular data from video traffic detection (VTD) are used for minimizing the travel delay at intersections and a real-time traffic optimization model, based on the SUMO traffic simulation software, is established accordingly. The proposed model is implemented in a small industrial control computer which serves as the communication interface between the traffic signal control system, the traffic simulation and optimization model and the real-time video traffic detecti...

  11. Dissection of the Influenza A Virus Endocytic Routes Reveals Macropinocytosis as an Alternative Entry Pathway

    Science.gov (United States)

    de Vries, Erik; Tscherne, Donna M.; Wienholts, Marleen J.; Cobos-Jiménez, Viviana; Scholte, Florine; García-Sastre, Adolfo; Rottier, Peter J. M.; de Haan, Cornelis A. M.

    2011-01-01

    Influenza A virus (IAV) enters host cells upon binding of its hemagglutinin glycoprotein to sialylated host cell receptors. Whereas dynamin-dependent, clathrin-mediated endocytosis (CME) is generally considered as the IAV infection pathway, some observations suggest the occurrence of an as yet uncharacterized alternative entry route. By manipulating entry parameters we established experimental conditions that allow the separate analysis of dynamin-dependent and -independent entry of IAV. Whereas entry of IAV in phosphate-buffered saline could be completely inhibited by dynasore, a specific inhibitor of dynamin, a dynasore-insensitive entry pathway became functional in the presence of fetal calf serum. This finding was confirmed with the use of small interfering RNAs targeting dynamin-2. In the presence of serum, both IAV entry pathways were operational. Under these conditions entry could be fully blocked by combined treatment with dynasore and the amiloride derivative EIPA, the hallmark inhibitor of macropinocytosis, whereas either drug alone had no effect. The sensitivity of the dynamin-independent entry pathway to inhibitors or dominant-negative mutants affecting actomyosin dynamics as well as to a number of specific inhibitors of growth factor receptor tyrosine kinases and downstream effectors thereof all point to the involvement of macropinocytosis in IAV entry. Consistently, IAV particles and soluble FITC-dextran were shown to co-localize in cells in the same vesicles. Thus, in addition to the classical dynamin-dependent, clathrin-mediated endocytosis pathway, IAV enters host cells by a dynamin-independent route that has all the characteristics of macropinocytosis. PMID:21483486

  12. Traffic sign recognition based on deep convolutional neural network

    Science.gov (United States)

    Yin, Shi-hao; Deng, Ji-cai; Zhang, Da-wei; Du, Jing-yuan

    2017-11-01

    Traffic sign recognition (TSR) is an important component of automated driving systems. It is a rather challenging task to design a high-performance classifier for the TSR system. In this paper, we propose a new method for TSR system based on deep convolutional neural network. In order to enhance the expression of the network, a novel structure (dubbed block-layer below) which combines network-in-network and residual connection is designed. Our network has 10 layers with parameters (block-layer seen as a single layer): the first seven are alternate convolutional layers and block-layers, and the remaining three are fully-connected layers. We train our TSR network on the German traffic sign recognition benchmark (GTSRB) dataset. To reduce overfitting, we perform data augmentation on the training images and employ a regularization method named "dropout". The activation function we employ in our network adopts scaled exponential linear units (SELUs), which can induce self-normalizing properties. To speed up the training, we use an efficient GPU to accelerate the convolutional operation. On the test dataset of GTSRB, we achieve the accuracy rate of 99.67%, exceeding the state-of-the-art results.

  13. Intelligent Traffic Light Based on PLC Control

    Science.gov (United States)

    Mei, Lin; Zhang, Lijian; Wang, Lingling

    2017-11-01

    The traditional traffic light system with a fixed control mode and single control function is contradicted with the current traffic section. The traditional one has been unable to meet the functional requirements of the existing flexible traffic control system. This paper research and develop an intelligent traffic light called PLC control system. It uses PLC as control core, using a sensor module for receiving real-time information of vehicles, traffic control mode for information to select the traffic lights. Of which control mode is flexible and changeable, and it also set the countdown reminder to improve the effectiveness of traffic lights, which can realize the goal of intelligent traffic diversion, intelligent traffic diversion.

  14. Traffic analysis and control using image processing

    Science.gov (United States)

    Senthilkumar, K.; Ellappan, Vijayan; Arun, A. R.

    2017-11-01

    This paper shows the work on traffic analysis and control till date. It shows an approach to regulate traffic the use of image processing and MATLAB systems. This concept uses computational images that are to be compared with original images of the street taken in order to determine the traffic level percentage and set the timing for the traffic signal accordingly which are used to reduce the traffic stoppage on traffic lights. They concept proposes to solve real life scenarios in the streets, thus enriching the traffic lights by adding image receivers like HD cameras and image processors. The input is then imported into MATLAB to be used. as a method for calculating the traffic on roads. Their results would be computed in order to adjust the traffic light timings on a particular street, and also with respect to other similar proposals but with the added value of solving a real, big instance.

  15. Traffic dynamics around weaving section influenced by accident: Cellular automata approach

    Science.gov (United States)

    Kong, Lin-Peng; Li, Xin-Gang; Lam, William H. K.

    2015-07-01

    The weaving section, as a typical bottleneck, is one source of vehicle conflicts and an accident-prone area. Traffic accident will block lanes and the road capacity will be reduced. Several models have been established to study the dynamics around traffic bottlenecks. However, little attention has been paid to study the complex traffic dynamics influenced by the combined effects of bottleneck and accident. This paper presents a cellular automaton model to characterize accident-induced traffic behavior around the weaving section. Some effective control measures are proposed and verified for traffic management under accident condition. The total flux as a function of inflow rates, the phase diagrams, the spatial-temporal diagrams, and the density and velocity profiles are presented to analyze the impact of accident. It was shown that the proposed control measures for weaving traffic can improve the capacity of weaving section under both normal and accident conditions; the accidents occurring on median lane in the weaving section are more inclined to cause traffic jam and reduce road capacity; the capacity of weaving section will be greatly reduced when the accident happens downstream the weaving section.

  16. The physics of traffic jams

    Science.gov (United States)

    Nagatani, Takashi

    2002-09-01

    Traffic flow is a kind of many-body system of strongly interacting vehicles. Traffic jams are a typical signature of the complex behaviour of vehicular traffic. Various models are presented to understand the rich variety of physical phenomena exhibited by traffic. Analytical and numerical techniques are applied to study these models. Particularly, we present detailed results obtained mainly from the microscopic car-following models. A typical phenomenon is the dynamical jamming transition from the free traffic (FT) at low density to the congested traffic at high density. The jamming transition exhibits the phase diagram similar to a conventional gas-liquid phase transition: the FT and congested traffic correspond to the gas and liquid phases, respectively. The dynamical transition is described by the time-dependent Ginzburg-Landau equation for the phase transition. The jamming transition curve is given by the spinodal line. The metastability exists in the region between the spinodal and phase separation lines. The jams in the congested traffic reveal various density waves. Some of these density waves show typical nonlinear waves such as soliton, triangular shock and kink. The density waves are described by the nonlinear wave equations: the Korteweg-de-Vries (KdV) equation, the Burgers equation and the Modified KdV equation. Subjects like the traffic flow such as bus-route system and pedestrian flow are touched as well. The bus-route system with many buses exhibits the bunching transition where buses bunch together with proceeding ahead. Such dynamic models as the car-following model are proposed to investigate the bunching transition and bus delay. A recurrent bus exhibits the dynamical transition between the delay and schedule-time phases. The delay transition is described in terms of the nonlinear map. The pedestrian flow also reveals the jamming transition from the free flow at low density to the clogging at high density. Some models are presented to study the

  17. Road traffic injuries in Colombia.

    Science.gov (United States)

    Rodríguez, Deysi Yasmin; Fernández, Francisco José; Acero Velásquez, Hugo

    2003-01-01

    Road traffic injuries are a leading public health problem in Colombia. Pedestrians are the most vulnerable road users, especially in the main urban centers of Bogotá, Medellin and Cali. Data analyzed in this report include official statistics from the National Police and the National Institute of Legal Medicine and Forensic Sciences for 1996-2000, and results of a study conducted at the National University of Colombia in 2000. Methods from the Highway Capacity Manual were used for determining physical and technical variables, and a Geographical Information System tool was used for the location and spatial analysis of the road traffic crashes. Pedestrians accounted for close to 32% of injuries and 40% of the deaths from road traffic crashes. The problem of road traffic crashes existed predominately in urban areas. In the main urban centers, pedestrians constituted nearly 68% of road traffic crash victims. The high level of risky road use behaviors demonstrated by pedestrians and drivers, and inadequate infrastructure for safe mobility of pedestrians in some sections of the road network were the main contributing factors. Major improvements were achieved in Bogotá following enhancements to the municipal transport system and other policies introduced since 1995. In conclusion, policies and programs for improving road safety, in particular pedestrian safety, and strengthening urban planning are top priority.

  18. Structuring of Road Traffic Flows

    Directory of Open Access Journals (Sweden)

    Planko Rožić

    2005-09-01

    Full Text Available Systemic traffic count on the Croatian road network hasbeen carried out for more than three decades in different ways.During this period a large number of automatic traffic countershave been installed, and they operate on different principles.The traffic count has been analyzed from the aspect of vehicleclassification. The count results can be only partly comparedsince they yield different structures of traffic flows. Special analysisrefers to the classification of vehicles by automatic trafficcounters.During the research, a database has been formed with physicalelements of vehicles of over five thousand vehicle types. Theresearch results prove that the vehicle length only is not sufficientfor the classification of vehicles, the way it is used in thepresent automatic traffic counts, but rather the number of axles,the wheelbase as well as the front and rear overhangs needto be considered as well. Therefore, the detector system shouldapply also the detector of axles.The results have been presented that were obtained as partof the program TEST- Technological, research, developmentproject supported by the Minist1y of Science, Education andSport.

  19. Simulation Model of Traffic Jam at Crossroads

    Directory of Open Access Journals (Sweden)

    Mladen Kalajžić

    2002-11-01

    Full Text Available Traffic congestion is one of the major problems in most cities.It is the consequence of unavoidable motorization, butalso, in many cases, of improper solutions considering constructionof roads or organisation of traffic.This paper deals with one problematic crossroad in thetown of Zadar in which traffic jams occur due to poor organisationof traffic. Using mathematical simulation, the first partproves that traffic jams will certainly occur, and in the secondpart, crossroads signalling is considered as a possible solutionwhich, if combined with intelligent control could significantlyimprove the organisation of traffic at this crossroads.

  20. Air traffic security act unconstitutional

    International Nuclear Information System (INIS)

    Heller, W.

    2006-01-01

    In the interest of more effective protective measures against terrorist attacks, the German federal parliament inter alia added a clause to the Air Traffic Security Act (Sec. 14, Para. 3, Air Traffic Security Act) empowering the armed forces to shoot down aircraft to be used as a weapon against human lives. In Germany, this defense possibility has been discussed also in connection with deliberate crashes of hijacked aircraft on nuclear power plants. The 1 st Division of the German Federal Constitutional Court, in its decision of February 15, 2006, ruled that Sec. 14, Para. 3, Air Traffic Security Act was incompatible with the Basic Law and thus was null and void (file No. 1 BvR 357/05) for two reasons: - There was no legislative authority on the part of the federal government. - The provision was incompatible with the basic right of life and the guarantee of human dignity as enshrined in the Basic Law. (orig.)

  1. On vehicular traffic data analysis

    Energy Technology Data Exchange (ETDEWEB)

    Brics, Martins; Mahnke, Reinhard [Institute of Physics, Rostock University (Germany)

    2011-07-01

    This contribution consists of analysis of empirical vehicular traffic flow data. The main focus lies on the Next Generation Simulation (NGSIM) data. The first findings show that there are artificial structures within the data due to errors of monitoring as well as smoothing position measurement data. As a result speed data show discretisation in 5 feet per second. The aim of this investigation is to construct microscopic traffic flow models which are in agreement to the analysed empirical data. The ongoing work follows the subject of research summarized by Christof Liebe in his PhD thesis entitled ''Physics of traffic flow: Empirical data and dynamical models'' (Rostock, 2010).

  2. Epigenetic modulation of the biophysical properties of drug-resistant cell lipids to restore drug transport and endocytic functions.

    Science.gov (United States)

    Vijayaraghavalu, Sivakumar; Peetla, Chiranjeevi; Lu, Shan; Labhasetwar, Vinod

    2012-09-04

    In our recent studies exploring the biophysical characteristics of resistant cell lipids, and the role they play in drug transport, we demonstrated the difference of drug-resistant breast cancer cells from drug-sensitive cells in lipid composition and biophysical properties, suggesting that cancer cells acquire a drug-resistant phenotype through the alteration of lipid synthesis to inhibit intracellular drug transport to protect from cytotoxic effect. In cancer cells, epigenetic changes (e.g., DNA hypermethylation) are essential to maintain this drug-resistant phenotype. Thus, altered lipid synthesis may be linked to epigenetic mechanisms of drug resistance. We hypothesize that reversing DNA hypermethylation in resistant cells with an epigenetic drug could alter lipid synthesis, changing the cell membrane's biophysical properties to facilitate drug delivery to overcome drug resistance. Herein we show that treating drug-resistant breast cancer cells (MCF-7/ADR) with the epigenetic drug 5-aza-2'-deoxycytidine (decitabine) significantly alters cell lipid composition and biophysical properties, causing the resistant cells to acquire biophysical characteristics similar to those of sensitive cell (MCF-7) lipids. Following decitabine treatment, resistant cells demonstrated increased sphingomyelinase activity, resulting in a decreased sphingomyelin level that influenced lipid domain structures, increased membrane fluidity, and reduced P-glycoprotein expression. Changes in the biophysical characteristics of resistant cell lipids facilitated doxorubicin transport and restored endocytic function for drug delivery with a lipid-encapsulated form of doxorubicin, enhancing the drug efficacy. In conclusion, we have established a new mechanism for efficacy of an epigenetic drug, mediated through changes in lipid composition and biophysical properties, in reversing cancer drug resistance.

  3. Agent Based Individual Traffic guidance

    DEFF Research Database (Denmark)

    Wanscher, Jørgen Bundgaard

    2004-01-01

    can be obtained through cellular phone tracking or GPS systems. This information can then be used to provide individual traffic guidance as opposed to the mass information systems of today -- dynamic roadsigns and trafficradio. The goal is to achieve better usage of road and time. The main topic......When working with traffic planning or guidance it is common practice to view the vehicles as a combined mass. >From this models are employed to specify the vehicle supply and demand for each region. As the models are complex and the calculations are equally demanding the regions and the detail...

  4. Intelligent Ambulance Traffic Assistance System

    OpenAIRE

    RONOJOY GHOSH; VIVEK SHAH; HITESH AGARWAL; ASHUTOSH BHUSHAN; PRASUN KANTI GHOSH

    2013-01-01

    With the increase in traffic road density, several causalities occur due to delay in taking a patient to the hospital in an ambulance. In this paper, we have developed an algorithm to find the shortest path to reach the required destination. As required the software will identify the present location of the vehicle and ask the user for the destination. Then it will show all the available paths, highlighting the shortest one or in several cases the most optimum one. Further we made the traffic...

  5. Traffic and Granular Flow '11

    CERN Document Server

    Buslaev, Alexander; Bugaev, Alexander; Yashina, Marina; Schadschneider, Andreas; Schreckenberg, Michael; TGF11

    2013-01-01

    This book continues the biannual series of conference proceedings, which has become a classical reference resource in traffic and granular research alike. It addresses new developments at the interface between physics, engineering and computational science. Complex systems, where many simple agents, be they vehicles or particles, give rise to surprising and fascinating phenomena.   The contributions collected in these proceedings cover several research fields, all of which deal with transport. Topics include highway, pedestrian and internet traffic, granular matter, biological transport, transport networks, data acquisition, data analysis and technological applications. Different perspectives, i.e. modeling, simulations, experiments and phenomenological observations, are considered.

  6. Right bundle branch block

    DEFF Research Database (Denmark)

    Bussink, Barbara E; Holst, Anders Gaarsdal; Jespersen, Lasse

    2013-01-01

    AimsTo determine the prevalence, predictors of newly acquired, and the prognostic value of right bundle branch block (RBBB) and incomplete RBBB (IRBBB) on a resting 12-lead electrocardiogram in men and women from the general population.Methods and resultsWe followed 18 441 participants included.......5%/2.3% in women, P Right bundle branch block was associated with significantly...... increased all-cause and cardiovascular mortality in both genders with age-adjusted hazard ratios (HR) of 1.31 [95% confidence interval (CI), 1.11-1.54] and 1.87 (95% CI, 1.48-2.36) in the gender pooled analysis with little attenuation after multiple adjustment. Right bundle branch block was associated...

  7. Wireless traffic steering for green cellular networks

    CERN Document Server

    Zhang, Shan; Zhou, Sheng; Niu, Zhisheng; Shen, Xuemin (Sherman)

    2016-01-01

    This book introduces wireless traffic steering as a paradigm to realize green communication in multi-tier heterogeneous cellular networks. By matching network resources and dynamic mobile traffic demand, traffic steering helps to reduce on-grid power consumption with on-demand services provided. This book reviews existing solutions from the perspectives of energy consumption reduction and renewable energy harvesting. Specifically, it explains how traffic steering can improve energy efficiency through intelligent traffic-resource matching. Several promising traffic steering approaches for dynamic network planning and renewable energy demand-supply balancing are discussed. This book presents an energy-aware traffic steering method for networks with energy harvesting, which optimizes the traffic allocated to each cell based on the renewable energy status. Renewable energy demand-supply balancing is a key factor in energy dynamics, aimed at enhancing renewable energy sustainability to reduce on-grid energy consum...

  8. Highway and traffic engineering in developing countries

    DEFF Research Database (Denmark)

    Jørgensen, N O

    1996-01-01

    Describes road and traffic engineering methods and problems in developing countries as opposed to similar problems in industrialized countries......Describes road and traffic engineering methods and problems in developing countries as opposed to similar problems in industrialized countries...

  9. Active Traffic Capture for Network Forensics

    Science.gov (United States)

    Slaviero, Marco; Granova, Anna; Olivier, Martin

    Network traffic capture is an integral part of network forensics, but current traffic capture techniques are typically passive in nature. Under heavy loads, it is possible for a sniffer to miss packets, which affects the quality of forensic evidence.

  10. Genetic algorithms for air traffic assignment

    OpenAIRE

    Delahaye , Daniel; Alliot , Jean-Marc; Schoenauer , Marc; Farges , Jean-Loup

    1994-01-01

    International audience; In this paper, we show how genetic algorithms can be used to compute automatically a traffic assignment of aircraft on the air network to increase Air Traffic Control capacity in high density areas.

  11. E-Block: A Tangible Programming Tool with Graphical Blocks

    OpenAIRE

    Danli Wang; Yang Zhang; Shengyong Chen

    2013-01-01

    This paper designs a tangible programming tool, E-Block, for children aged 5 to 9 to experience the preliminary understanding of programming by building blocks. With embedded artificial intelligence, the tool defines the programming blocks with the sensors as the input and enables children to write programs to complete the tasks in the computer. The symbol on the programming block's surface is used to help children understanding the function of each block. The sequence information is transfer...

  12. Oxidation by neutrophils-derived HOCl increases immunogenicity of proteins by converting them into ligands of several endocytic receptors involved in antigen uptake by dendritic cells and macrophages.

    Directory of Open Access Journals (Sweden)

    Rafał Biedroń

    Full Text Available The initiation of adaptive immune responses to protein antigens has to be preceded by their uptake by antigen presenting cells and intracellular proteolytic processing. Paradoxically, endocytic receptors involved in antigen uptake do not bind the majority of proteins, which may be the main reason why purified proteins stimulate at most weak immune responses. A shared feature of different types of adjuvants, capable of boosting immunogenicity of protein vaccines, is their ability to induce acute inflammation, characterized by early influx of activated neutrophils. Neutrophils are also rapidly recruited to sites of tissue injury or infection. These cells are the source of potent oxidants, including hypochlorous acid (HOCl, causing oxidation of proteins present in inflammatory foci. We demonstrate that oxidation of proteins by endogenous, neutrophils-derived HOCl increases their immunogenicity. Upon oxidation, different, randomly chosen simple proteins (yeast alcohol dehydrogenase, human and bovine serum albumin and glycoproteins (human apo-transferrin, ovalbumin gain the ability to bind with high affinity to several endocytic receptors on antigen presenting cells, which seems to be the major mechanism of their increased immunogenicity. The mannose receptor (CD206, scavenger receptors A (CD204 and CD36 were responsible for the uptake and presentation of HOCl-modified proteins by murine dendritic cells and macrophages. Other scavenger receptors, SREC-I and LOX-1, as well as RAGE were also able to bind HOCl-modified proteins, but they did not contribute significantly to these ligands uptake by dendritic cells because they were either not expressed or exhibited preference for more heavily oxidised proteins. Our results indicate that oxidation by neutrophils-derived HOCl may be a physiological mechanism of conferring immunogenicity on proteins which in their native forms do not bind to endocytic receptors. This mechanism might enable the immune system

  13. Theoretical Considerations of Interdisciplinary Expertise in Traffic

    Directory of Open Access Journals (Sweden)

    Zdravko Peran

    2012-10-01

    Full Text Available Jnterdisciplinwy traffic expertise is being increasingly appliedin road traffic. It is not possible to determine whether anaccident had been intentionally set up without such expertise.The interdisciplinary expertise is the result of mutual work ofseveral different interdisciplinary experts. The paper analysesthe basic characteristics of interdisciplinary investigation ofset-up traffic accidents. Special attention has been paid to interdisciplinwyexpertise of set-up traffic accidents involving injuredpersons or fatalities.

  14. Holonic Models for Traffic Control Systems

    Science.gov (United States)

    Ciufudean, Calin; Filote, Constantin

    This paper proposes a new time-placed net model for traffic control systems, respectively railway control traffic systems. This model can be interpreted as a holonic one, and contains three modules: Transport Planning Module, Transport Control Module and Priority Control Module. For railway traffic systems we introduce a strategy in a timed-place Petri net model to solve collision and traffic jam problems.

  15. Some random models in traffic science

    Energy Technology Data Exchange (ETDEWEB)

    Hjorth, U.

    1996-06-01

    We give an overview of stochastic models for the following traffic phenomena. Models for traffic flow including gaps and capacities for lanes, crossings and roundabouts. Models for wanted and achieved speed distributions. Mode selection models including dispersed equilibrium models and traffic accident models. Also some statistical questions are discussed. 60 refs, 1 tab

  16. Predicting Information Flows in Network Traffic.

    Science.gov (United States)

    Hinich, Melvin J.; Molyneux, Robert E.

    2003-01-01

    Discusses information flow in networks and predicting network traffic and describes a study that uses time series analysis on a day's worth of Internet log data. Examines nonlinearity and traffic invariants, and suggests that prediction of network traffic may not be possible with current techniques. (Author/LRW)

  17. Empirical analysis of heterogeneous traffic flow

    NARCIS (Netherlands)

    Ambarwati, L.; Pel, A.J.; Verhaeghe, R.J.; Van Arem, B.

    2013-01-01

    Traffic flow in many developing countries is strongly mixed comprising vehicle types, such as motorcycles, cars, (mini) buses, and trucks; furthermore, traffic flow typically exhibits free inter-lane exchanges. This phenomenon causes a complex vehicle interaction, rendering most existing traffic

  18. Models for Predictive Railway Traffic Management

    NARCIS (Netherlands)

    Kecman, P.

    2014-01-01

    The potential growth in transport demand in the next decade and beyond requires a change from reactive to proactive traffic control to maintain and improve the reliability of railway traffic. In order to enable an anticipative approach to traffic management, it is necessary to develop the tools for

  19. NEW POSSIBILITIES OF RAILWAY TRAFFIC CONTROL SYSTEMS

    Directory of Open Access Journals (Sweden)

    Lionginas LIUDVINAVIČIUS

    2016-06-01

    Full Text Available This article analyses the train traffic control systems in 1435 mm and 1520 mm gauge railways. The article analyses the aspects of train traffic control and locomotive energy saving by using the coordinates of track profile change that have been received from GPS. In the article, achievements of Lithuanian railways (LG in the area of train traffic control optimisation are presented.

  20. Light signals for road traffic control.

    NARCIS (Netherlands)

    Schreuder, D.A.

    1981-01-01

    Signals for road traffic control are a major constituent of the modern traffic scene, particularly in built-up areas. A vast amount of research has been executed in the last two decennia, resulting in a fairly generally accepted view on what the requirements for effective traffic lights are. For the

  1. 30 CFR 56.9100 - Traffic control.

    Science.gov (United States)

    2010-07-01

    ... 30 Mineral Resources 1 2010-07-01 2010-07-01 false Traffic control. 56.9100 Section 56.9100 Mineral Resources MINE SAFETY AND HEALTH ADMINISTRATION, DEPARTMENT OF LABOR METAL AND NONMETAL MINE... Dumping Traffic Safety § 56.9100 Traffic control. To provide for the safe movement of self-propelled...

  2. ReFlow: Reports on Internet Traffic

    NARCIS (Netherlands)

    Hoogesteger, Martijn; de Oliveira Schmidt, R.; Sperotto, Anna; Pras, Aiko

    Internet traffic statistics can provide valuable information to network analysts and researchers about the traffic, technologies and main characteristics of today’s networks. For many years Internet2 maintained a public website with statistics about the traffic in the Abilene network. This site was

  3. TRAFFIC SIGN RECOGNATION WITH VIDEO PROCESSING TECHNIQUE

    Directory of Open Access Journals (Sweden)

    Musa AYDIN

    2013-01-01

    Full Text Available In this study, traffic signs are aimed to be recognized and identified from a video image which is taken through a video camera. To accomplish our aim, a traffic sign recognition program has been developed in MATLAB/Simulink environment. The target traffic sign are recognized in the video image with the developed program.

  4. Phase diagram distortion from traffic parameter averaging.

    NARCIS (Netherlands)

    Stipdonk, H. Toorenburg, J. van & Postema, M.

    2010-01-01

    Motorway traffic congestion is a major bottleneck for economic growth. Therefore, research of traffic behaviour is carried out in many countries. Although well describing the undersaturated free flow phase as an almost straight line in a (k,q)-phase diagram, congested traffic observations and

  5. Capturing Internet Traffic Dynamics through Graph Distances

    NARCIS (Netherlands)

    Uhlig, S.; Fu, B.; Jamakovic, A.

    2009-01-01

    Studies of the Internet have typically focused either on the routing system, i.e. the paths chosen to reach a given destination, or on the evolution of traffic on a physical link. In this paper, we combine routing and traffic, and study for the first time the evolution of the traffic on the Internet

  6. An auxiliary graph based dynamic traffic grooming algorithm in spatial division multiplexing enabled elastic optical networks with multi-core fibers

    Science.gov (United States)

    Zhao, Yongli; Tian, Rui; Yu, Xiaosong; Zhang, Jiawei; Zhang, Jie

    2017-03-01

    A proper traffic grooming strategy in dynamic optical networks can improve the utilization of bandwidth resources. An auxiliary graph (AG) is designed to solve the traffic grooming problem under a dynamic traffic scenario in spatial division multiplexing enabled elastic optical networks (SDM-EON) with multi-core fibers. Five traffic grooming policies achieved by adjusting the edge weights of an AG are proposed and evaluated through simulation: maximal electrical grooming (MEG), maximal optical grooming (MOG), maximal SDM grooming (MSG), minimize virtual hops (MVH), and minimize physical hops (MPH). Numeric results show that each traffic grooming policy has its own features. Among different traffic grooming policies, an MPH policy can achieve the lowest bandwidth blocking ratio, MEG can save the most transponders, and MSG can obtain the fewest cores for each request.

  7. Multi-Layer Traffic Steering

    DEFF Research Database (Denmark)

    Fotiadis, Panagiotis; Polignano, Michele; Gimenez, Lucas Chavarria

    2013-01-01

    This paper investigates the potentials of traffic steering in the Radio Resource Control (RRC) Idle state by evaluating the Absolute Priorities (AP) framework in a multilayer Long Term Evolution (LTE) macrocell scenario. Frequency priorities are broadcast on the system information and RRC Idle...

  8. Next generation traffic management centers.

    Science.gov (United States)

    2013-05-01

    Traffic management centers (TMCs) are critical to providing mobility to millions of people travelling on high-volume roadways. In Virginia, as with most regions of the United States, TMCs were aggressively deployed in the late 1990s and early 2000s. ...

  9. Traffic at risk in Mediterranean

    International Nuclear Information System (INIS)

    Bilardo, U.; Mureddu, G.

    1993-01-01

    The Mediterranean Sea represents only about 0.7% of the planet's total water surface area, yet it is host to as much as one-quarter of the world's total maritime oil traffic. Statistics indicate that from 47 to 77,000 tonnes of crude oil are now being released annually into the Sea through accidental spills; and over the last decade, its tourism dependent coastlines have been fouled by the highest levels of tar contamination in the world. Oil carrier traffic, routed within the Sea's already overcrowded shipping lanes, is intense and this traffic is expected to increase, as a result of rises in world energy demand, to levels of from 7 to 8 million barrels a day. It has been estimated that, at the end of 1992, 90% of all large tankers operating in this area, will have reached a service life of 15-16 years which is very close to the average recommended life cycle limit of 15-20 years. Only 20% of the world's 3,000 tankers are currently equipped with double bottomed hulls. This paper uses these and other facts and figures to argue that the risks of future severe oil tanker accidents in the Mediterranean Sea are high, and that these must be countered with the development of a new set of stricter marine traffic safety regulations at the Italian, national, as well as, European level

  10. Statistical variances in traffic data

    OpenAIRE

    Krbalek, Milan; Seba, Petr

    2006-01-01

    We perform statistical analysis of the single-vehicle data measured on the Dutch freeway A9 and discussed in Ref. [2]. Using tools originating from the Random Matrix Theory we show that the significant changes in the statistics of the traffic data can be explained applying equilibrium statistical physics of interacting particles.

  11. Learning to Detect Traffic Signs

    DEFF Research Database (Denmark)

    Møgelmose, Andreas; Trivedi, Mohan M.; Moeslund, Thomas B.

    2012-01-01

    This study compares the performance of sign detection based on synthetic training data to the performance of detection based on real-world training images. Viola-Jones detectors are created for 4 different traffic signs with both synthetic and real data, and varying numbers of training samples. T...

  12. 2008 Michigan traffic crash facts

    Science.gov (United States)

    2009-03-18

    In keeping with recent trends, traffic fatalities in 2008 were down to 980, a 9.6 : percent decrease from last year. The total number of persons injured also declined : 7.5 percent to 74,568 and total crashes dropped 2.5 percent to 316,057. Most : no...

  13. 2009 Michigan traffic crash facts

    Science.gov (United States)

    2010-01-01

    In keeping with recent trends, traffic fatalities in 2009 were down to 871, a 11.1 : percent decrease from last year. The total number of persons injured also declined : 4.9 percent to 70,931 and total crashes dropped 7.9 percent to 290,978. Most : n...

  14. Variations in urban traffic volumes

    NARCIS (Netherlands)

    Thomas, Tom; Weijermars, Wendy; van Berkum, Eric C.

    2008-01-01

    Over the past few decades passenger transport has grown rapidly resulting in a multitude of problems including severe traffic congestion and pollution. It is expected that passenger transport will continue to grow rapidly in the future, which will worsen the situation even further. The traditional

  15. An antibody toolkit for the study of membrane traffic in Drosophila melanogaster

    Directory of Open Access Journals (Sweden)

    Falko Riedel

    2016-07-01

    Full Text Available The use of Drosophila melanogaster as a model organism has been pivotal to understanding the developmental processes of metazoans. However, the use of flies for studying subcellular organization is hampered by a paucity of reliable reagents to label specific organelles. Here, we describe the generation of mouse monoclonal antibodies against a set of markers of the secretory and endocytic pathways, along with goat polyclonal antibodies against two Golgi proteins. We show that the monoclonal antibodies are highly specific and sufficiently sensitive to detect endogenous proteins in crude extracts by immunoblotting with little background staining. By immunofluorescence the major compartments of the membrane traffic system (including the endoplasmic reticulum, the Golgi, and early and late endosomes are labeled by at least one antibody. Moreover, the antibodies can be used to label organelles in fly tissues including salivary glands and wing imaginal discs. We anticipate that these antibodies will provide a useful tool kit to facilitate the investigation of how the endomembrane system functions and varies in the diverse tissue types of metazoans.

  16. Bacteria-Containing Vacuoles: Subversion of Cellular Membrane Traffic and Autophagy.

    Science.gov (United States)

    Tang, Bor Luen

    2015-01-01

    Intracellular bacterial pathogens drive the formation of host membrane-derived pseudo-organelles that facilitate their replication, survival, or dormancy. The formation and maintenance of these bacteria-containing vacuoles (BCVs) are dependent on the bacteria's ability to usurp the host's intracellular membrane system, in particular dynamic compartments involved in exo-/endocytic membrane traffic and autophagy. Bacteria are typically internalized by phagocytosis, and the compartment matures through endosomal fusion. The bacteria-containing phagosome/endosome often becomes the base for BCV formation. Diverse strategies used by different bacterial pathogens prevent the BCV from being destroyed via the endolysosomal pathway. Furthermore, bacterial survival or proliferation in BCVs could be augmented by host membrane transport processes subverted by secreted bacterial factors, which facilitate the acquisition of membrane sources and nutrients. BCVs may be targeted for destruction by autophagy, and various facultative and obligate intracellular bacteria have evolved ways to evade or even exploit autophagy. Here we review examples of bacterial subversion of host cellular membrane transport and autophagy machinery for a productive invasion.

  17. Contaminated soil concrete blocks

    NARCIS (Netherlands)

    de Korte, A.C.J.; Brouwers, Jos; Limbachiya, Mukesh C.; Kew, Hsein Y.

    2009-01-01

    According to Dutch law the contaminated soil needs to be remediated or immobilised. The main focus in this article is the design of concrete blocks, containing contaminated soil, that are suitable for large production, financial feasible and meets all technical and environmental requirements. In

  18. Making Block Grants Accountable.

    Science.gov (United States)

    Chelimsky, Eleanor

    Methods of accountability are presented in considering the Reagan administration plan to consolidate 84 federal health, education and social service grants into six block grant areas and to cut overall funding. After matching aspects of public criticism with proposal objectives, a rationale is developed for building elements of accountability into…

  19. Linoleum Block Printing Revisited.

    Science.gov (United States)

    Chetelat, Frank J.

    1980-01-01

    The author discusses practical considerations of teaching linoleum block printing in the elementary grades (tool use, materials, motivation) and outlines a sequence of design concepts in this area for the primary, intermediate and junior high grades. A short list of books and audiovisual aids is appended. (SJL)

  20. Effects of Block Scheduling

    Directory of Open Access Journals (Sweden)

    William R. Veal

    1999-09-01

    Full Text Available This study examined the effects of a tri-schedule on the academic achievement of students in a high school. The tri-schedule consists of traditional, 4x4 block, and hybrid schedules running at the same time in the same high school. Effectiveness of the schedules was determined from the state mandated test of basic skills in reading, language, and mathematics. Students who were in a particular schedule their freshman year were tested at the beginning of their sophomore year. A statistical ANCOVA test was performed using the schedule types as independent variables and cognitive skill index and GPA as covariates. For reading and language, there was no statistically significant difference in test results. There was a statistical difference mathematics-computation. Block mathematics is an ideal format for obtaining more credits in mathematics, but the block format does little for mathematics achievement and conceptual understanding. The results have content specific implications for schools, administrations, and school boards who are considering block scheduling adoption.

  1. Coding with Blockly

    CERN Document Server

    Lovett, Amber

    2017-01-01

    "Blockly is a fun, graphical programming language designed to get kids interested in creating their own computer programs. Through simple text written to foster creativity and problem solving, students will the art of innovation. Large, colorful images show students how to complete activities. Additional tools, including a glossary and an index, help students learn new vocabulary and locate information."-- Provided by publisher.

  2. [Masquerading bundle branch block].

    Science.gov (United States)

    Kukla, Piotr; Baranchuk, Adrian; Jastrzębski, Marek; Bryniarski, Leszek

    2014-01-01

    We here describe a surface 12-lead electrocardiogram (ECG) of a 72-year-old female with a prior history of breast cancer and chemotherapy-induced cardiomyopathy. An echocardiogram revealed left ventricular dysfunction, ejection fraction of 23%, with mild enlarged left ventricle. The 12-lead ECG showed atrial fibrillation with a mean heart rate of about 100 bpm, QRS duration 160 ms, QT interval 400 ms, right bundle branch block (RBBB) and left anterior fascicular block (LAFB). The combination of RBBB features in the precordial leads and LAFB features in the limb leads is known as ''masquerading bundle branch block''. In most cases of RBBB and LAFB, the QRS axis deviation is located between - 80 to -120 degrees. Rarely, when predominant left ventricular forces are present, the QRS axis deviation is near about -90 degrees, turning the pattern into an atypical form. In a situation of RBBB associated with LAFB, the S wave can be absent or very small in lead I. Such a situation is the result of not only purely LAFB but also with left ventricular hypertrophy and/or focal block due to scar (extensive anterior myocardial infarction) or fibrosis (cardiomyopathy). Sometimes, this specific ECG pattern is mistaken for LBBB. RBBB with LAFB may imitate LBBB either in the limb leads (known as 'standard masquerading' - absence of S wave in lead I), or in the precordial leads (called 'precordial masquerading' - absence of S wave in leads V₅ and V₆). Our ECG showed both these types of masquerading bundle branch block - absence of S wave in lead I and in leads V₅ and V₆.

  3. Car Delay Model near Bus Stops with Mixed Traffic Flow

    Directory of Open Access Journals (Sweden)

    Yang Xiaobao

    2013-01-01

    Full Text Available This paper proposes a model for estimating car delays at bus stops under mixed traffic using probability theory and queuing theory. The roadway is divided to serve motorized and nonmotorized traffic streams. Bus stops are located on the nonmotorized lanes. When buses dwell at the stop, they block the bicycles. Thus, two conflict points between car stream and other traffic stream are identified. The first conflict point occurs as bicycles merge to the motorized lane to avoid waiting behind the stopping buses. The second occurs as buses merge back to the motorized lane. The average car delay is estimated as the sum of the average delay at these two conflict points and the delay resulting from following the slower bicycles that merged into the motorized lane. Data are collected to calibrate and validate the developed model from one site in Beijing. The sensitivity of car delay to various operation conditions is examined. The results show that both bus stream and bicycle stream have significant effects on car delay. At bus volumes above 200 vehicles per hour, the curbside stop design is not appropriate because of the long car delays. It can be replaced by the bus bay design.

  4. Evolution of Traffic Jam in Traffic Flow Model

    Science.gov (United States)

    Fukui, Minoru; Ishibashi, Yoshihiro

    1993-11-01

    Traffic flow is simulated in a three-state cellular automaton model. In a two-dimensional cell without a crashed car, the ensemble average of the velocity of the cars is enhanced by the self-organization in the low-density phase of cars. In the high-density phase above p{=}0.5 of car density, the velocity is decreased and the system then degenerates into a global jamming phase in which all cars are stopped. A crashed car provides the seed of a jamming cluster, which grows into a global traffic jam even in the low-density phase. The growth of the jamming cluster is studied, and the time dependence of the number of jamming cars and the scaling law for the cell sizes are discussed.

  5. The car following model considering traffic jerk

    Science.gov (United States)

    Ge, Hong-Xia; Zheng, Peng-jun; Wang, Wei; Cheng, Rong-Jun

    2015-09-01

    Based on optimal velocity car following model, a new model considering traffic jerk is proposed to describe the jamming transition in traffic flow on a highway. Traffic jerk means the sudden braking and acceleration of vehicles, which has a significant impact on traffic movement. The nature of the model is researched by using linear and nonlinear analysis method. A thermodynamic theory is formulated to describe the phase transition and critical phenomenon in traffic flow. The time-dependent Ginzburg-Landau (TDGL) equation and the modified Korteweg-de Vries (mKdV) equation are derived to describe the traffic flow near the critical point and the traffic jam. In addition, the connection between the TDGL and the mKdV equations are also given.

  6. In Situ Peroxidase Labeling and Mass-Spectrometry Connects Alpha-Synuclein Directly to Endocytic Trafficking and mRNA Metabolism in Neurons.

    Science.gov (United States)

    Chung, Chee Yeun; Khurana, Vikram; Yi, Song; Sahni, Nidhi; Loh, Ken H; Auluck, Pavan K; Baru, Valeriya; Udeshi, Namrata D; Freyzon, Yelena; Carr, Steven A; Hill, David E; Vidal, Marc; Ting, Alice Y; Lindquist, Susan

    2017-02-22

    Synucleinopathies, including Parkinson's disease (PD), are associated with the misfolding and mistrafficking of alpha-synuclein (α-syn). Here, using an ascorbate peroxidase (APEX)-based labeling method combined with mass spectrometry, we defined a network of proteins in the immediate vicinity of α-syn in living neurons to shed light on α-syn function. This approach identified 225 proteins, including synaptic proteins, proteins involved in endocytic vesicle trafficking, the retromer complex, phosphatases and mRNA binding proteins. Many were in complexes with α-syn, and some were encoded by genes known to be risk factors for PD and other neurodegenerative diseases. Endocytic trafficking and mRNA translation proteins within this spatial α-syn map overlapped with genetic modifiers of α-syn toxicity, developed in an accompanying study (Khurana et al., this issue of Cell Systems). Our data suggest that perturbation of these particular pathways is directly related to the spatial localization of α-syn within the cell. These approaches provide new avenues to systematically examine protein function and pathology in living cells. Copyright © 2017 Elsevier Inc. All rights reserved.

  7. SUPERFICIAL CERVICAL PLEXUS BLOCK

    Directory of Open Access Journals (Sweden)

    Komang Mega Puspadisari

    2014-01-01

    Full Text Available Superficial cervical plexus block is one of the regional anesthesia in  neck were limited to thesuperficial fascia. Anesthesia is used to relieve pain caused either during or after the surgery iscompleted. This technique can be done by landmark or with ultrasound guiding. The midpointof posterior border of the Sternocleidomastoid was identified and the prosedure done on thatplace or on the level of cartilage cricoid.

  8. Change Around the Block?

    Science.gov (United States)

    Berlin, Joey

    2017-04-01

    Proponents of a block grant or per-capita cap trumpet them as vehicles for the federal government to give the states a capped amount of funding for Medicaid that legislatures would effectively distribute how they see fit. Questions abound as to what capped Medicaid funding would look like, and what effect it would have on the current Medicaid-eligible population, covered services, and physician payments.

  9. Managing access block.

    Science.gov (United States)

    Cameron, Peter; Scown, Paul; Campbell, Donald

    2002-01-01

    There is pessimism regarding the ability of the Acute Health Sector to manage access block for emergency and elective patients. Melbourne Health suffered an acute bed crisis in 2001 resulting in record ambulance diversions and emergency department (ED) delays. We conducted an observational study to reduce access block for emergency patients whilst maintaining elective throughput at Melbourne Health. This involved a clinician-led taskforce using previously proven principles for organisational change to implement 51 actions to improve patient access over a three-month period. The primary outcome measures were ambulance diversion, emergency patients waiting more than 12 hours for an inpatient bed, elective throughput and theatre cancellations. Despite a reduction in multi-day bed numbers all primary objectives were met, ambulance diversion decreased to minimal levels, 12-hour waits decreased by 40% and elective throughput was maintained. Theatre cancellations were also minimised. We conclude that access block can be improved by clinician-led implementation of proven process improvements over a short time frame. The ability to sustain change over the longer term requires further study.

  10. Agent Based Individual Traffic Guidance

    DEFF Research Database (Denmark)

    Wanscher, Jørgen

    This thesis investigates the possibilities in applying Operations Research (OR) to autonomous vehicular traffic. The explicit difference to most other research today is that we presume that an agent is present in every vehicle - hence Agent Based Individual Traffic guidance (ABIT). The next...... evolutionary step for the in-vehicle route planners is the introduction of two-way communication. We presume that the agent is capable of exactly this. Based on this presumption we discuss the possibilities and define a taxonomy and use this to discuss the ABIT system. Based on a set of scenarios we conclude...... that the system can be divided into two separate constituents. The immediate dispersion, which is used for small areas and quick response, and the individual alleviation, which considers the longer distance decision support. Both of these require intrinsicate models and cost functions which at the beginning...

  11. Modelling traffic pollution in streets

    Energy Technology Data Exchange (ETDEWEB)

    Berkowicz, R.; Hertel, O. [National Environmental Research Inst., Dept. of Atmospheric Environment, Roskilde (Denmark); Larsen, S.E.; Soerensen, N.N.; Nielsen, M. [Risoe National Lab., Dept. of Meteorology and Wind Energy, Roskilde (Denmark)

    1997-01-01

    This report concerns mainly the subject related to modelling air pollution from traffic in urban streets. A short overview is presented over the theoretical aspects and examples of most commonly used methods and models are given. Flow and dispersion conditions in street canyons are discussed and the presentation is substantiated with the analysis of the experimental data. The main emphasis is on the modelling methods that are suitable for routine applications and a more detailed presentation is given of the Operational Street Pollution Model (OSPM), which was developed by the National Environmental Research Institute. The model is used for surveillance of air pollution from traffic in Danish cities and also for special air pollution studies. (au) 76 refs.

  12. Container Traffic In European Ports

    Directory of Open Access Journals (Sweden)

    Elen Twrdy

    2004-03-01

    Full Text Available Over the last fifteen years the European transport markethas witnessed a growth of container traffic which today reachesapproximately 50 million TEU per year. From 1997 to 2002,container traffic in the northern European ports increased from14 to 20.6 million TEU per year, in the ports of the westernMedite"anean from 6 to 10 million TEU per year, and in thenorthern Adriatic ports from 0. 69 to 0. 74 million TEU per year.The ports of the northern Adriatic are located in three states(Slovenia, Croatia and Italy with different statuses in relationto the common European market. In addition, different developmentlevels of these states are reflected in different levels ofinternational commercial exchange, the development of the existinginfrastructure and plans for the construction of new infrastructures.However, all three countries share a common goaltoincrease their competitiveness in comparison with the westemEuropean ports.

  13. Crowding effects in vehicular traffic.

    Directory of Open Access Journals (Sweden)

    Jay Samuel L Combinido

    Full Text Available While the impact of crowding on the diffusive transport of molecules within a cell is widely studied in biology, it has thus far been neglected in traffic systems where bulk behavior is the main concern. Here, we study the effects of crowding due to car density and driving fluctuations on the transport of vehicles. Using a microscopic model for traffic, we found that crowding can push car movement from a superballistic down to a subdiffusive state. The transition is also associated with a change in the shape of the probability distribution of positions from a negatively-skewed normal to an exponential distribution. Moreover, crowding broadens the distribution of cars' trap times and cluster sizes. At steady state, the subdiffusive state persists only when there is a large variability in car speeds. We further relate our work to prior findings from random walk models of transport in cellular systems.

  14. Traffic control: nursing practice calendar.

    Science.gov (United States)

    Rus, Linda; Cheesebro, Kathy; Nagra, Erica; Neff, Alaina

    2013-01-01

    Educating nurses on the multitude of new and updated best practices, changes in regulatory standards, new equipment, and enhanced technology creates an "information traffic jam." Multiple practice changes occurring simultaneously pose challenges for nurses to retain information to practice safely and effectively. An absence of coordination between various nursing and allied health teaching initiatives compounds this problem. A nursing practice calendar was developed to facilitate the prioritization, communication, and education of hospital-wide initiatives affecting nursing practice.

  15. Active Traffic Management in Michigan

    OpenAIRE

    Johnson, Pat

    2018-01-01

    The US 23 Flex Route is the first active traffic management (ATM) project in the state of Michigan. This route utilizes overhead lane control gantries equipped with various intelligent transportation system (ITS) equipment to facilitate the following ATM strategies: dynamic shoulder use, dynamic lane control, variable speed advisories, and queue warning. The focus of this presentation is how the project team overcame several challenges during the planning, design, and system management phases...

  16. Traffic Conflict Detection and Resolution (CDR) in ERTMS System

    OpenAIRE

    Zhu, Taomei

    2016-01-01

    This thesis addresses the conflict problem in railway traffic. Although the railway traffic management nowadays can partly be carried out by a traffic control system (or a traffic management system), the control system is insufficient to make operational decisions once the occurrence of disturbance gets frequent, particularly at the junctions with dense traffic. Traffic conflicts may arise along with the disturbances. The traffic controllers (and dispatchers) are often required to handle thes...

  17. Paving block study : final report.

    Science.gov (United States)

    1971-10-01

    The Louisiana Department of Highways has conducted field tests with an experimental revetment consisting of cellular concrete revetment blocks used in conjunction with plastic filter cloth and/or vegetation such as grass or vines. The precast blocks ...

  18. Habitat Blocks and Wildlife Corridors

    Data.gov (United States)

    Vermont Center for Geographic Information — Habitat blocks are areas of contiguous forest and other natural habitats that are unfragmented by roads, development, or agriculture. Vermonts habitat blocks are...

  19. Demographic Data - MDC_Block

    Data.gov (United States)

    NSGIC Local Govt | GIS Inventory — A polygon feature class of Miami-Dade Census 2000 Blocks. Census blocks are areas bounded on all sides by visible and/or invisible features shown on a map prepared...

  20. Blocking the Hawking radiation

    DEFF Research Database (Denmark)

    Autzen, M.; Kouvaris, C.

    2014-01-01

    grows after its formation (and eventually destroys the star) instead of evaporating. The fate of the black hole is dictated by the two opposite mechanics, i.e., accretion of nuclear matter from the center of the star and Hawking radiation that tends to decrease the mass of the black hole. We study how...... the assumptions for the accretion rate can in fact affect the critical mass beyond which a black hole always grows. We also study to what extent degenerate nuclear matter can impede Hawking radiation due to the fact that emitted particles can be Pauli blocked at the core of the star....

  1. How Artists Overcome Creative Blocks.

    Science.gov (United States)

    Hirst, Barbara

    1992-01-01

    Six practicing artists were interviewed about how they overcome creative blocks. Their responses indicated that feelings of self-doubt, fear, and depression accompany blocks but that relaxing and working on new directions and playing ideas off a supportive person helped to overcome such blocks. (DB)

  2. Block Scheduling in High Schools.

    Science.gov (United States)

    Irmsher, Karen

    1996-01-01

    Block Scheduling has been considered a cure for a lengthy list of educational problems. This report reviews the literature on block schedules and describes some Oregon high schools that have integrated block scheduling. Major disadvantages included resistance to change and requirements that teachers change their teaching strategies. There is…

  3. Abdominal wall blocks in adults

    DEFF Research Database (Denmark)

    Neimann, Jens Dupont Børglum; Gögenür, Ismail; Bendtsen, Thomas F.

    2016-01-01

    Purpose of review Abdominal wall blocks in adults have evolved much during the last decade; that is, particularly with the introduction of ultrasound-guided (USG) blocks. This review highlights recent advances of block techniques within this field and proposes directions for future research.  Rec...

  4. Analysis of vehicular traffic flow in the major areas of Kuala Lumpur utilizing open-traffic

    Science.gov (United States)

    Manogaran, Saargunawathy; Ali, Muhammad; Yusof, Kamaludin Mohamad; Suhaili, Ramdhan

    2017-09-01

    Vehicular traffic congestion occurs when a large number of drivers are overcrowded on the road and the traffic flow does not run smoothly. Traffic congestion causes chaos on the road and interruption to daily activities of users. Time consumed on road give lots of negative effects on productivity, social behavior, environmental and cost to economy. Congestion is worsens and leads to havoc during the emergency such as flood, accidents, road maintenance and etc., where behavior of traffic flow is always unpredictable and uncontrollable. Real-time and historical traffic data are critical inputs for most traffic flow analysis applications. Researcher attempt to predict traffic using simulations as there is no exact model of traffic flow exists due to its high complexity. Open Traffic is an open source platform available for traffic data analysis linked to Open Street Map (OSM). This research is aimed to study and understand the Open Traffic platform. The real-time traffic flow pattern in Kuala Lumpur area was successfully been extracted and analyzed using Open Traffic. It was observed that the congestion occurs on every major road in Kuala Lumpur and most of it owes to the offices and the economic and commercial centers during rush hours. At some roads the congestion occurs at night due to the tourism activities.

  5. Traffic and Granular Flow ’07

    CERN Document Server

    Chevoir, François; Gondret, Philippe; Lassarre, Sylvain; Lebacque, Jean-Patrick; Schreckenberg, Michael

    2009-01-01

    This book covers several research fields, all of which deal with transport. Three main topics are treated: road traffic, granular matter, and biological transport. Different points of view, i.e. modelling, simulations, experiments, and phenomenological observations, are considered. Sub-topics include: highway or urban vehicular traffic (dynamics of traffic, macro/micro modelling, measurements, data analysis, security issues, psychological issues), pedestrian traffic, animal traffic (e.g. social insects), collective motion in biological systems (molecular motors...), granular flow (dense flows, intermittent flows, solid/liquid transition, jamming, force networks, fluid and solid friction), networks (biological networks, urban traffic, the internet, vulnerability of networks, optimal transport networks) and cellular automata applied to the various aforementioned fields.

  6. 11th Traffic and Granular Flow Conference

    CERN Document Server

    Daamen, Winnie

    2016-01-01

    The Conference on Traffic and Granular Flow brings together international researchers from different fields ranging from physics to computer science and engineering to discuss the latest developments in traffic-related systems. Originally conceived to facilitate new ideas by considering the similarities of traffic and granular flow, TGF'15, organised by Delft University of Technology, now covers a broad range of topics related to driven particle and transport systems. Besides the classical topics of granular flow and highway traffic, its scope includes data transport (Internet traffic), pedestrian and evacuation dynamics, intercellular transport, swarm behaviour and the collective dynamics of other biological systems. Recent advances in modelling, computer simulation and phenomenology are presented, and prospects for applications, for example to traffic control, are discussed. The conference explores the interrelations between the above-mentioned fields and offers the opportunity to stimulate interdisciplinar...

  7. A System for Traffic Violation Detection

    Directory of Open Access Journals (Sweden)

    Nourdine Aliane

    2014-11-01

    Full Text Available This paper describes the framework and components of an experimental platform for an advanced driver assistance system (ADAS aimed at providing drivers with a feedback about traffic violations they have committed during their driving. The system is able to detect some specific traffic violations, record data associated to these faults in a local data-base, and also allow visualization of the spatial and temporal information of these traffic violations in a geographical map using the standard Google Earth tool. The test-bed is mainly composed of two parts: a computer vision subsystem for traffic sign detection and recognition which operates during both day and nighttime, and an event data recorder (EDR for recording data related to some specific traffic violations. The paper covers firstly the description of the hardware architecture and then presents the policies used for handling traffic violations.

  8. Traffic Flow Management Wrap-Up

    Science.gov (United States)

    Grabbe, Shon

    2011-01-01

    Traffic Flow Management involves the scheduling and routing of air traffic subject to airport and airspace capacity constraints, and the efficient use of available airspace. Significant challenges in this area include: (1) weather integration and forecasting, (2) accounting for user preferences in the Traffic Flow Management decision making process, and (3) understanding and mitigating the environmental impacts of air traffic on the environment. To address these challenges, researchers in the Traffic Flow Management area are developing modeling, simulation and optimization techniques to route and schedule air traffic flights and flows while accommodating user preferences, accounting for system uncertainties and considering the environmental impacts of aviation. This presentation will highlight some of the major challenges facing researchers in this domain, while also showcasing recent innovations designed to address these challenges.

  9. Classification and Analysis of Computer Network Traffic

    DEFF Research Database (Denmark)

    Bujlow, Tomasz

    2014-01-01

    for traffic classification, which can be used for nearly real-time processing of big amounts of data using affordable CPU and memory resources. Other questions are related to methods for real-time estimation of the application Quality of Service (QoS) level based on the results obtained by the traffic...... to create realistic traffic profiles of the selected applications, which can server as the training data for MLAs. We assessed the usefulness of C5.0 Machine Learning Algorithm (MLA) in the classification of computer network traffic. We showed that the application-layer payload is not needed to train the C5......Traffic monitoring and analysis can be done for multiple different reasons: to investigate the usage of network resources, assess the performance of network applications, adjust Quality of Service (QoS) policies in the network, log the traffic to comply with the law, or create realistic models...

  10. Traffic analysis toolbox volume XI : weather and traffic analysis, modeling and simulation.

    Science.gov (United States)

    2010-12-01

    This document presents a weather module for the traffic analysis tools program. It provides traffic engineers, transportation modelers and decisions makers with a guide that can incorporate weather impacts into transportation system analysis and mode...

  11. Real-Time Corrected Traffic Correlation Model for Traffic Flow Forecasting

    Directory of Open Access Journals (Sweden)

    Hua-pu Lu

    2015-01-01

    Full Text Available This paper focuses on the problems of short-term traffic flow forecasting. The main goal is to put forward traffic correlation model and real-time correction algorithm for traffic flow forecasting. Traffic correlation model is established based on the temporal-spatial-historical correlation characteristic of traffic big data. In order to simplify the traffic correlation model, this paper presents correction coefficients optimization algorithm. Considering multistate characteristic of traffic big data, a dynamic part is added to traffic correlation model. Real-time correction algorithm based on Fuzzy Neural Network is presented to overcome the nonlinear mapping problems. A case study based on a real-world road network in Beijing, China, is implemented to test the efficiency and applicability of the proposed modeling methods.

  12. Seat belt, DWI, and other traffic violations among recent immigrants in Florida and Tennessee : traffic tech.

    Science.gov (United States)

    2013-05-01

    The rapidly changing racial and ethnic composition of the : U.S. population is important to traffic safety specialists : because involvement in fatal traffic crashes varies across : racial and ethnic groups. Some research suggests that certain : mino...

  13. Program structure-based blocking

    Science.gov (United States)

    Bertolli, Carlo; Eichenberger, Alexandre E.; O'Brien, John K.; Sura, Zehra N.

    2017-09-26

    Embodiments relate to program structure-based blocking. An aspect includes receiving source code corresponding to a computer program by a compiler of a computer system. Another aspect includes determining a prefetching section in the source code by a marking module of the compiler. Yet another aspect includes performing, by a blocking module of the compiler, blocking of instructions located in the prefetching section into instruction blocks, such that the instruction blocks of the prefetching section only contain instructions that are located in the prefetching section.

  14. Traffic Management for Satellite-ATM Networks

    Science.gov (United States)

    Goyal, Rohit; Jain, Raj; Fahmy, Sonia; Vandalore, Bobby; Goyal, Mukul

    1998-01-01

    Various issues associated with "Traffic Management for Satellite-ATM Networks" are presented in viewgraph form. Specific topics include: 1) Traffic management issues for TCP/IP based data services over satellite-ATM networks; 2) Design issues for TCP/IP over ATM; 3) Optimization of the performance of TCP/IP over ATM for long delay networks; and 4) Evaluation of ATM service categories for TCP/IP traffic.

  15. The Technical Issues Of Traffic Analysis

    Directory of Open Access Journals (Sweden)

    Pavel Vladimirovich Yegorov

    2016-03-01

    Full Text Available The main problem in the analysis of Internet traffic associated with a large number of network applications, complex patterns of communication and a significant amounts of information. The definition of the category of traffic using the analysis of the number of port is irrelevant for P2P applications, streaming data and many other types of network applications. The article describes some of technical issues related to the analysis of Internet traffic.

  16. Respiratory symptoms in children living near busy roads and their relationship to vehicular traffic: results of an Italian multicenter study (SIDRIA 2

    Directory of Open Access Journals (Sweden)

    Dell'Orco Valerio

    2009-06-01

    Full Text Available Abstract Background Epidemiological studies have provided evidence that exposure to vehicular traffic increases the prevalence of respiratory symptoms and may exacerbate pre-existing asthma in children. Self-reported exposure to road traffic has been questioned as a reliable measurement of exposure to air pollutants. The aim of this study was to investigate whether there were specific effects of cars and trucks traffic on current asthma symptoms (i.e. wheezing and cough or phlegm, and to examine the validity of self-reported traffic exposure. Methods The survey was conducted in 2002 in 12 centers in Northern, Center and Southern Italy, different in size, climate, latitude and level of urbanization. Standardized questionnaires filled in by parents were used to collect information on health outcomes and exposure to traffic among 33,632 6–7 and 13–14 years old children and adolescents. Three questions on traffic exposure were asked: the traffic in the zone of residence, the frequency of truck and of car traffic in the street of residence. The presence of a possible response bias for the self-reported traffic was evaluated using external validation (comparison with measurements of traffic flow in the city of Turin and internal validations (matching by census block, in the cities of Turin, Milan and Rome. Results Overall traffic density was weakly associated with asthma symptoms but there was a stronger association with cough or phlegm (high traffic density OR = 1.24; 95% CI: 1.04, 1.49. Car and truck traffic were independently associated with cough or phlegm. The results of the external validation did not support the existence of a reporting bias for the observed associations, for all the self-reported traffic indicators examined. The internal validations showed that the observed association between traffic density in the zone of residence and respiratory symptoms did not appear to be explained by an over reporting of traffic by parents of

  17. The negative effects of homogeneous traffic on merging sections

    NARCIS (Netherlands)

    Elbers, Bart; van Berkum, Eric C.

    2003-01-01

    Homogeneous traffic flows are believed to be better in absorbing disturbances, raise capacity and stimulate traffic safety. Measures to make traffic more homogeneous are therefore often taken to increase capacity. This paper shows that the ability of a traffic flow to deal with traffic coming from

  18. Traffic light control by multiagent reinforcement learning systems

    NARCIS (Netherlands)

    Bakker, B.; Whiteson, S.; Kester, L.; Groen, F.C.A.; Babuška, R.; Groen, F.C.A.

    2010-01-01

    Traffic light control is one of the main means of controlling road traffic. Improving traffic control is important because it can lead to higher traffic throughput and reduced traffic congestion. This chapter describes multiagent reinforcement learning techniques for automatic optimization of

  19. Traffic Light Control by Multiagent Reinforcement Learning Systems

    NARCIS (Netherlands)

    Bakker, B.; Whiteson, S.; Kester, L.J.H.M.; Groen, F.C.A.

    2010-01-01

    Traffic light control is one of the main means of controlling road traffic. Improving traffic control is important because it can lead to higher traffic throughput and reduced traffic congestion. This chapter describes multiagent reinforcement learning techniques for automatic optimization of

  20. On traffic modelling in GPRS networks

    DEFF Research Database (Denmark)

    Madsen, Tatiana Kozlova; Schwefel, Hans-Peter; Prasad, Ramjee

    2005-01-01

    Optimal design and dimensioning of wireless data networks, such as GPRS, requires the knowledge of traffic characteristics of different data services. This paper presents an in-detail analysis of an IP-level traffic measurements taken in an operational GPRS network. The data measurements reported...... here are done at the Gi interface. The aim of this paper is to reveal some key statistics of GPRS data applications and to validate if the existing traffic models can adequately describe traffic volume and inter-arrival time distribution for different services. Additionally, we present a method of user...

  1. A queuing model for road traffic simulation

    International Nuclear Information System (INIS)

    Guerrouahane, N.; Aissani, D.; Bouallouche-Medjkoune, L.; Farhi, N.

    2015-01-01

    We present in this article a stochastic queuing model for the raod traffic. The model is based on the M/G/c/c state dependent queuing model, and is inspired from the deterministic Godunov scheme for the road traffic simulation. We first propose a variant of M/G/c/c state dependent model that works with density-flow fundamental diagrams rather than density-speed relationships. We then extend this model in order to consider upstream traffic demand as well as downstream traffic supply. Finally, we show how to model a whole raod by concatenating raod sections as in the deterministic Godunov scheme

  2. Air traffic management evaluation tool

    Science.gov (United States)

    Sridhar, Banavar (Inventor); Sheth, Kapil S. (Inventor); Chatterji, Gano Broto (Inventor); Bilimoria, Karl D. (Inventor); Grabbe, Shon (Inventor); Schipper, John F. (Inventor)

    2012-01-01

    Methods for evaluating and implementing air traffic management tools and approaches for managing and avoiding an air traffic incident before the incident occurs. A first system receives parameters for flight plan configurations (e.g., initial fuel carried, flight route, flight route segments followed, flight altitude for a given flight route segment, aircraft velocity for each flight route segment, flight route ascent rate, flight route descent route, flight departure site, flight departure time, flight arrival time, flight destination site and/or alternate flight destination site), flight plan schedule, expected weather along each flight route segment, aircraft specifics, airspace (altitude) bounds for each flight route segment, navigational aids available. The invention provides flight plan routing and direct routing or wind optimal routing, using great circle navigation and spherical Earth geometry. The invention provides for aircraft dynamics effects, such as wind effects at each altitude, altitude changes, airspeed changes and aircraft turns to provide predictions of aircraft trajectory (and, optionally, aircraft fuel use). A second system provides several aviation applications using the first system. Several classes of potential incidents are analyzed and averted, by appropriate change en route of one or more parameters in the flight plan configuration, as provided by a conflict detection and resolution module and/or traffic flow management modules. These applications include conflict detection and resolution, miles-in trail or minutes-in-trail aircraft separation, flight arrival management, flight re-routing, weather prediction and analysis and interpolation of weather variables based upon sparse measurements. The invention combines these features to provide an aircraft monitoring system and an aircraft user system that interact and negotiate changes with each other.

  3. Block copolymer investigations

    Science.gov (United States)

    Yufa, Nataliya A.

    The research presented in this thesis deals with various aspects of block copolymers on the nanoscale: their behavior at a range of temperatures, their use as scaffolds, or for creation of chemically striped surfaces, as well as the behavior of metals on block copolymers under the influence of UV light, and the healing behavior of copolymers. Invented around the time of World War II, copolymers have been used for decades due to their macroscopic properties, such as their ability to be molded without vulcanization, and the fact that, unlike rubber, they can be recycled. In recent years, block copolymers (BCPs) have been used for lithography, as scaffolds for nano-objects, to create a magnetic hard drive, as well as in photonic and other applications. In this work we used primarily atomic force microscopy (AFM) and transmission electron microscopy (TEM), described in Chapter II, to conduct our studies. In Chapter III we demonstrate a new and general method for positioning nanoparticles within nanoscale grooves. This technique is suitable for nanodots, nanocrystals, as well as DNA. We use AFM and TEM to demonstrate selective decoration. In Chapters IV and V we use AFM and TEM to study the structure of polymer surfaces coated with metals and self-assembled monolayers. We describe how the surfaces were created, exhibit their structure on the nanoscale, and prove that their macroscopic wetting properties have been altered compared to the original polymer structures. Finally, Chapters VI and VII report out in-situ AFM studies of BCP at high temperatures, made possible only recently with the invention of air-tight high-temperature AFM imaging cells. We locate the transition between disordered films and cylinders during initial ordering. Fluctuations of existing domains leading to domain coarsening are also described, and are shown to be consistent with reptation and curvature minimization. Chapter VII deals with the healing of PS-b-PMMA following AFM-tip lithography or

  4. Traffic Information Unit, Traffic Information System, Vehicle Management System, Vehicle, and Method of Controlling a Vehicle

    NARCIS (Netherlands)

    Papp, Z.; Doodeman, G.J.N.; Nelisse, M.W.; Sijs, J.; Theeuwes, J.A.C.; Driessen, B.J.F.

    2010-01-01

    A traffic information unit (MD1, MD2, MD3) according to the invention comprises a facility (MI) for tracking vehicle state information of individual vehicles present at a traffic infrastructure and a facility (T) for transmitting said vehicle state information to a vehicle (70B, 70E). A traffic

  5. Forecasting Multivariate Road Traffic Flows Using Bayesian Dynamic Graphical Models, Splines and Other Traffic Variables

    NARCIS (Netherlands)

    Anacleto, Osvaldo; Queen, Catriona; Albers, Casper J.

    Traffic flow data are routinely collected for many networks worldwide. These invariably large data sets can be used as part of a traffic management system, for which good traffic flow forecasting models are crucial. The linear multiregression dynamic model (LMDM) has been shown to be promising for

  6. Travelers ability to observe changes in traffic intensities and traffic light settings

    NARCIS (Netherlands)

    Vreeswijk, Jacob Dirk; Do, Michael; Middag, Wilco; Martens, Marieke Hendrikje; van Berkum, Eric C.; van Arem, Bart; ITSC,

    2011-01-01

    Travel choice behavior is an important determinant in traffic and subject to human imperfection and bounded rationality. In decision-making processes travelers seldom act perfectly rational. Traffic models and traffic network management measure could become more realistic and effective, if

  7. Remark on the Causes of Traffic Accidents and Traffic Awareness: Examples of Usak Province

    Directory of Open Access Journals (Sweden)

    Ercan ÖZEN

    2014-01-01

    Full Text Available Traffic accidents cause important losses on the social and economics structure of countries. While economic losses are able to be compensated in a short time, it isn’t possible to compensate social and communal losses. This study tackles with not the economic aspect of traffic accidents but the social dimension, evaluates the causes of the traffic accidents with respect to views of individuals on traffic and aims to measure the levels of awareness of individuals about traffic. To achieve these goals, a group of 506 people were surveyed in Uşak province and the acquired data were analyzed via one-way ANOVA test in SPSS 16 program. According to the analysis results, drivers and pedestrians are considered to have significant flaws in traffic accidents. But alongside this thought, the idea that traffic accidents are caused by the road having technical and physical problems has come to the fore. This results present a valid motive for the established approach “Vision Zero” , being practised in Sweden, to be adopted in Turkey. When the level of awareness about traffic is examined, it is seen that individuals generally have got inadequate information related traffic rules and traffic authorities. Besides, the fact that individuals are inadequate in terms of courtesy and respect in traffic and that more serious efforts on traffic education is needed to be spent come out as the important findings of this study. The findings of this study can be thought as a directive tool to the policy makers.

  8. Scaling in Computer Network Traffic

    Science.gov (United States)

    2005-01-07

    behaviour, measurement .... 29 The Self-Similar Traffic Model Fractional Gaussian Noise (fGn) and Fractional Brownian Motion (fBm) The unique...AVAILABILITY STATEMENT Approved for public release, distribution unlimited 13. SUPPLEMENTARY NOTES See also ADM001750, Wavelets and Multifractal Analysis... Multifractality Wavelet qth order moments: IE|dX(j, k)|q ∼ C 2αqj, j → 0. Estimating the LHS from data using Sq(j) = 1 nj ∑ k |dX(j, k)|q, and measure the slopes

  9. Multicore-Optimized Wavefront Diamond Blocking for Optimizing Stencil Updates

    KAUST Repository

    Malas, T.

    2015-07-02

    The importance of stencil-based algorithms in computational science has focused attention on optimized parallel implementations for multilevel cache-based processors. Temporal blocking schemes leverage the large bandwidth and low latency of caches to accelerate stencil updates and approach theoretical peak performance. A key ingredient is the reduction of data traffic across slow data paths, especially the main memory interface. In this work we combine the ideas of multicore wavefront temporal blocking and diamond tiling to arrive at stencil update schemes that show large reductions in memory pressure compared to existing approaches. The resulting schemes show performance advantages in bandwidth-starved situations, which are exacerbated by the high bytes per lattice update case of variable coefficients. Our thread groups concept provides a controllable trade-off between concurrency and memory usage, shifting the pressure between the memory interface and the CPU. We present performance results on a contemporary Intel processor.

  10. Celiac ganglia block

    Energy Technology Data Exchange (ETDEWEB)

    Akinci, Devrim [Department of Radiology, Hacettepe University School of Medicine, Sihhiye, 06100 Ankara (Turkey); Akhan, Okan [Department of Radiology, Hacettepe University School of Medicine, Sihhiye, 06100 Ankara (Turkey)]. E-mail: oakhan@hacettepe.edu.tr

    2005-09-01

    Pain occurs frequently in patients with advanced cancers. Tumors originating from upper abdominal viscera such as pancreas, stomach, duodenum, proximal small bowel, liver and biliary tract and from compressing enlarged lymph nodes can cause severe abdominal pain, which do not respond satisfactorily to medical treatment or radiotherapy. Percutaneous celiac ganglia block (CGB) can be performed with high success and low complication rates under imaging guidance to obtain pain relief in patients with upper abdominal malignancies. A significant relationship between pain relief and degree of tumoral celiac ganglia invasion according to CT features was described in the literature. Performing the procedure in the early grades of celiac ganglia invasion on CT can increase the effectiveness of the CGB, which is contrary to World Health Organization criteria stating that CGB must be performed in patients with advanced stage cancer. CGB may also be effectively performed in patients with chronic pancreatitis for pain palliation.

  11. Photovoltaic building blocks

    DEFF Research Database (Denmark)

    Hanberg, Peter Jesper; Jørgensen, Anders Michael

    2014-01-01

    efficiency of about 15% for commercial Silicon solar cells there is still much to gain. DTU Danchip provides research facilities, equipment and expertise for the building blocks that comprises fabricating the efficient solar cell. In order to get more of the sun light into the device we provide thin film......Photovoltaics (PV), better known as solar cells, are now a common day sight on many rooftops in Denmark.The installed capacity of PV systems worldwide is growing exponentially1 and is the third most importantrenewable energy source today. The cost of PV is decreasing fast with ~10%/year but to make...... it directcompetitive with fossil energy sources a further reduction is needed. By increasing the efficiency of the solar cells one gain an advantage through the whole chain of cost. So that per produced Watt of power less material is spent, installation costs are lower, less area is used etc. With an average...

  12. Atomic Basic Blocks

    Science.gov (United States)

    Scheler, Fabian; Mitzlaff, Martin; Schröder-Preikschat, Wolfgang

    Die Entscheidung, einen zeit- bzw. ereignisgesteuerten Ansatz für ein Echtzeitsystem zu verwenden, ist schwierig und sehr weitreichend. Weitreichend vor allem deshalb, weil diese beiden Ansätze mit äußerst unterschiedlichen Kontrollflussabstraktionen verknüpft sind, die eine spätere Migration zum anderen Paradigma sehr schwer oder gar unmöglich machen. Wir schlagen daher die Verwendung einer Zwischendarstellung vor, die unabhängig von der jeweils verwendeten Kontrollflussabstraktion ist. Für diesen Zweck verwenden wir auf Basisblöcken basierende Atomic Basic Blocks (ABB) und bauen darauf ein Werkzeug, den Real-Time Systems Compiler (RTSC) auf, der die Migration zwischen zeit- und ereignisgesteuerten Systemen unterstützt.

  13. Celiac ganglia block

    International Nuclear Information System (INIS)

    Akinci, Devrim; Akhan, Okan

    2005-01-01

    Pain occurs frequently in patients with advanced cancers. Tumors originating from upper abdominal viscera such as pancreas, stomach, duodenum, proximal small bowel, liver and biliary tract and from compressing enlarged lymph nodes can cause severe abdominal pain, which do not respond satisfactorily to medical treatment or radiotherapy. Percutaneous celiac ganglia block (CGB) can be performed with high success and low complication rates under imaging guidance to obtain pain relief in patients with upper abdominal malignancies. A significant relationship between pain relief and degree of tumoral celiac ganglia invasion according to CT features was described in the literature. Performing the procedure in the early grades of celiac ganglia invasion on CT can increase the effectiveness of the CGB, which is contrary to World Health Organization criteria stating that CGB must be performed in patients with advanced stage cancer. CGB may also be effectively performed in patients with chronic pancreatitis for pain palliation

  14. The endocytic pathways of a secretory granule membrane protein in HEK293 cells: PAM and EGF traverse a dynamic multivesicular body network together.

    Science.gov (United States)

    Bäck, Nils; Kanerva, Kristiina; Kurutihalli, Vishwanatha; Yanik, Andrew; Ikonen, Elina; Mains, Richard E; Eipper, Betty A

    2017-08-01

    Peptidylglycine α-amidating monooxygenase (PAM) is highly expressed in neurons and endocrine cells, where it catalyzes one of the final steps in the biosynthesis of bioactive peptides. PAM is also expressed in unicellular organisms such as Chlamydomonas reinhardtii, which do not store peptides in secretory granules. As for other granule membrane proteins, PAM is retrieved from the cell surface and returned to the trans-Golgi network. This pathway involves regulated entry of PAM into multivesicular body intralumenal vesicles (ILVs). The aim of this study was defining the endocytic pathways utilized by PAM in cells that do not store secretory products in granules. Using stably transfected HEK293 cells, endocytic trafficking of PAM was compared to that of the mannose 6-phosphate (MPR) and EGF (EGFR) receptors, established markers for the endosome to trans-Golgi network and degradative pathways, respectively. As in neuroendocrine cells, PAM internalized by HEK293 cells accumulated in the trans-Golgi network. Based on surface biotinylation, >70% of the PAM on the cell surface was recovered intact after a 4h chase and soluble, bifunctional PAM was produced. Endosomes containing PAM generally contained both EGFR and MPR and ultrastructural analysis confirmed that all three cargos accumulated in ILVs. PAM containing multivesicular bodies made frequent dynamic tubular contacts with younger and older multivesicular bodies. Frequent dynamic contacts were observed between lysosomes and PAM containing early endosomes and multivesicular bodies. The ancient ability of PAM to localize to ciliary membranes, which release bioactive ectosomes, may be related to its ability to accumulate in ILVs and exosomes. Copyright © 2017 Elsevier GmbH. All rights reserved.

  15. Bcl-xL regulates CD1d-mediated antigen presentation to NKT cells by altering CD1d trafficking through the endocytic pathway.

    Science.gov (United States)

    Subrahmanyam, Priyanka B; Carey, Gregory B; Webb, Tonya J

    2014-09-01

    NKT cells are a unique subset of T cells that recognize glycolipid Ags presented in the context of CD1d molecules. NKT cells mount strong antitumor responses and are a major focus in developing effective cancer immunotherapy. It is known that CD1d molecules are constantly internalized from the cell surface, recycled through the endocytic compartments, and re-expressed on the cell surface. However, little is known about the regulation of CD1d-mediated Ag processing and presentation in B cell lymphoma. Prosurvival factors of the Bcl-2 family, such as Bcl-xL, are often upregulated in B cell lymphomas and are intimately linked to sphingolipid metabolism, as well as the endocytic compartments. We hypothesized that Bcl-xL can regulate CD1d-mediated Ag presentation to NKT cells. We found that overexpression or induction of Bcl-xL led to increased Ag presentation to NKT cells. Conversely, the inhibition or knockdown of Bcl-xL led to decreased NKT cell activation. Furthermore, knockdown of Bcl-xL resulted in the loss of CD1d trafficking to lysosome-associated membrane protein 1(+) compartments. Rab7, a late endosomal protein, was upregulated and CD1d molecules accumulated in the Rab7(+) late endosomal compartment. These results demonstrate that Bcl-xL regulates CD1d-mediated Ag processing and presentation to NKT cells by altering the late endosomal compartment and changing the intracellular localization of CD1d. Copyright © 2014 by The American Association of Immunologists, Inc.

  16. Nitrogen deposition and traffic. State-of-the-art

    International Nuclear Information System (INIS)

    Teeuwisse, S.

    2010-01-01

    Nitrogen deposition is caused, among other things, by road traffic. This article addresses the relation between traffic and nitrogen deposition and the manner in which the contribution of traffic to nitrogen deposition can be quantified. [nl

  17. Symbols and warrants for major traffic generator guide signing.

    Science.gov (United States)

    2009-09-01

    The Texas Manual on Uniform Traffic Control Devices (TMUTCD) provides the definition of regular traffic generators based on four population types but not for major traffic generators (MTGs). MTG signs have been considered to supplement the overall si...

  18. Traffic flow characteristic and capacity in intelligent work zones.

    Science.gov (United States)

    2009-10-15

    Intellgent transportation system (ITS) technologies are utilized to manage traffic flow and safety in : highway work zones. Traffic management plans for work zones require queuing analyses to determine : the anticipated traffic backups, but the predi...

  19. Advanced Traffic Management Systems (ATMS) research analysis database system

    Science.gov (United States)

    2001-06-01

    The ATMS Research Analysis Database Systems (ARADS) consists of a Traffic Software Data Dictionary (TSDD) and a Traffic Software Object Model (TSOM) for application to microscopic traffic simulation and signal optimization domains. The purpose of thi...

  20. An analysis of the traffic safety phenomenon.

    NARCIS (Netherlands)

    Asmussen, E. & Kranenburg, A.

    1982-01-01

    The lack of traffic safety is a combination of the critical coincidence of circumstances in the traffic of incidents (near-accidents) and accidents with unwanted (permanent) consequences, such as fatalities, injured and disabled persons and material damage. This definition covers the whole of the

  1. Formation and Propagation of Local Traffic Jam

    Directory of Open Access Journals (Sweden)

    Hong-sheng Qi

    2013-01-01

    Full Text Available Large scale traffic congestion often stems from local traffic jam in single road or intersection. In this paper, macroscopic method was used to explore the formation and propagation of local traffic jam. It is found that (1 the propagation of traffic jam can be seen as the propagation of traffic signal parameters, that is, virtual split and virtual green time; (2 for a road with endogenous flow, entrance location influences the jam propagation. With the same demand (upstream links flow and entrance flow, the upstream got more influence; (3 when a one-lane road is thoroughly congested, virtual signal parameters everywhere are the same as that at stop line; for a basic road, the virtual signals work in a cooperative manner; (4 phase sequence is one important parameter that influences traffic performances during peak hour where spill back of channelization takes place. The same phase plan for left-turn flow and through flow would be preferred; (5 signal coordination plays an important role in traffic jam propagation and hence effective network signal parameters should be designed to prevent jam from propagation to the whole network. These findings would serve as a basis for future network traffic congestion control.

  2. Distraction-related road traffic collisions

    African Journals Online (AJOL)

    Abstract. Objectives: We aimed to prospectively study distraction-related road traffic collision injuries, their contributory factors, severity, and outcome. Methods: Data were prospectively collected on all hospitalized road traffic collision trauma patients in Al-Ain City who were drivers at the collision time over one and half years ...

  3. Symbols for cockpit displays of traffic information

    Science.gov (United States)

    2009-10-25

    A web-based study assessed pilots ability to learn and remember traffic symbols that may be shown on a Cockpit Display of Traffic Information (CDTI). These displays convey data obtained from Automatic Dependent Surveillance-Broadcast (ADS B) and rela...

  4. GPS queues with heterogeneous traffic classes

    NARCIS (Netherlands)

    Borst, Sem; Mandjes, M.R.H.; van Uitert, Miranda

    2002-01-01

    We consider a queue fed by a mixture of light-tailed and heavy-tailed traffic. The two traffic classes are served in accordance with the generalized processor sharing (GPS) discipline. GPS-based scheduling algorithms, such as weighted fair queueing (WFQ), have emerged as an important mechanism for

  5. 36 CFR 1004.13 - Obstructing traffic.

    Science.gov (United States)

    2010-07-01

    ... road, except as authorized by the Executive Director, or in the event of an accident or other condition... 36 Parks, Forests, and Public Property 3 2010-07-01 2010-07-01 false Obstructing traffic. 1004.13 Section 1004.13 Parks, Forests, and Public Property PRESIDIO TRUST VEHICLES AND TRAFFIC SAFETY § 1004.13...

  6. 36 CFR 4.13 - Obstructing traffic.

    Science.gov (United States)

    2010-07-01

    ... VEHICLES AND TRAFFIC SAFETY § 4.13 Obstructing traffic. The following are prohibited: (a) Stopping or parking a vehicle upon a park road, except as authorized by the superintendent, or in the event of an accident or other condition beyond the control of the operator. (b) Operating a vehicle so slowly as to...

  7. Environmental protection and traffic regulation in Slovenia

    Directory of Open Access Journals (Sweden)

    Aljaš Plevnik

    1998-01-01

    Full Text Available The article summarises the proposed chapter on traffic in the National programme for environmental protection. The main theme focuses on the suggested goals and measures to reach sustainable development in Slovenia. The goals and measures are derived from the current situation and traffic development trends and their influence on the environment, from international guidelines and the current Slovene policies.

  8. Floating car data for traffic monitoring

    DEFF Research Database (Denmark)

    Torp, Kristian; Lahrmann, Harry Spaabæk

    2005-01-01

    This paper describes a complete prototype system that uses Floating Car Data (FCD) for both automatic and manual detection of queues in traffic. The system is developed under EU’s Tempo program. The systems consists of small hardware units placed in mobile traffic report units (we use taxis...

  9. Visual Analysis in Traffic & Re-identification

    DEFF Research Database (Denmark)

    Møgelmose, Andreas

    Automated analysis of traffic situations - be it cars, signs, or pedestrians - is becoming increasingly relevant and feasible with the advent of powerful sensors, computers, and algorithms. This PhD thesis tackles three themes within this realm: Traffic sign detection, pedestrian detection...

  10. Gaussian queues in light and heavy traffic

    NARCIS (Netherlands)

    Dębicki, K.; Kosiński, K.M.; Mandjes, M.

    2012-01-01

    In this paper we investigate Gaussian queues in the light-traffic and in the heavy-traffic regime. Let $Q^{(c)}_{X}\\equiv\\{Q^{(c)}_{X}(t):t\\ge0\\}$ denote a stationary buffer content process for a fluid queue fed by the centered Gaussian process X≡{X(t):t∈ℝ} with stationary increments, X(0)=0,

  11. 7110.65H Air Traffic Control

    Science.gov (United States)

    1993-09-16

    these functions, it is rec. 2-75a2 Example.- ommended combining them on local control. The ATIS may be "Attention all aircraft. False air traffic...all aircraft. False air traffic control instructions used to specify the desired frequency. have been received in the area of Long Beach Airport

  12. Proportional green time scheduling for traffic lights

    NARCIS (Netherlands)

    P. Kovacs; Le, T. (Tung); R. Núñez Queija (Rudesindo); Vu, H. (Hai); N. Walton

    2016-01-01

    textabstractWe consider the decentralized scheduling of a large number of urban traffic lights. We investigate factors determining system performance, in particular, the length of the traffic light cycle and the proportion of green time allocated to each junction. We study the effect of the length

  13. Stochastic models for road traffic control

    NARCIS (Netherlands)

    Kovács, P.

    2016-01-01

    In this dissertation we make use of the theories of stochastic processes and operations research to develop models and methods to be applied for the analysis and control of road traffic networks. Within this field three subjects are considered: individual routing, urban traffic light networks and

  14. Traffic calming schemes : opportunities and implementation strategies.

    NARCIS (Netherlands)

    Schagen, I.N.L.G. van (ed.)

    2003-01-01

    Commissioned by the Swedish National Road Authority, this report aims to provide a concise overview of knowledge of and experiences with traffic calming schemes in urban areas, both on a technical level and on a policy level. Traffic calming refers to a combination of network planning and

  15. Dynamic traffic assignment on parallel computers

    Energy Technology Data Exchange (ETDEWEB)

    Nagel, K.; Frye, R.; Jakob, R.; Rickert, M.; Stretz, P.

    1998-12-01

    The authors describe part of the current framework of the TRANSIMS traffic research project at the Los Alamos National Laboratory. It includes parallel implementations of a route planner and a microscopic traffic simulation model. They present performance figures and results of an offline load-balancing scheme used in one of the iterative re-planning runs required for dynamic route assignment.

  16. resource allocation methodology for internet heterogeneous traffic

    African Journals Online (AJOL)

    Dr Obe

    buffer capacity in switches - that are required for heterogeneous internet traffic which guarantees a given QoS, even under high network loading conditions. This paper, therefore, presents a method for determining the optimum internet resources required for heterogeneous (data and voice only) traffic services to guarantee ...

  17. Will Automated Vehicles Negatively Impact Traffic Flow?

    Directory of Open Access Journals (Sweden)

    S. C. Calvert

    2017-01-01

    Full Text Available With low-level vehicle automation already available, there is a necessity to estimate its effects on traffic flow, especially if these could be negative. A long gradual transition will occur from manual driving to automated driving, in which many yet unknown traffic flow dynamics will be present. These effects have the potential to increasingly aid or cripple current road networks. In this contribution, we investigate these effects using an empirically calibrated and validated simulation experiment, backed up with findings from literature. We found that low-level automated vehicles in mixed traffic will initially have a small negative effect on traffic flow and road capacities. The experiment further showed that any improvement in traffic flow will only be seen at penetration rates above 70%. Also, the capacity drop appeared to be slightly higher with the presence of low-level automated vehicles. The experiment further investigated the effect of bottleneck severity and truck shares on traffic flow. Improvements to current traffic models are recommended and should include a greater detail and understanding of driver-vehicle interaction, both in conventional and in mixed traffic flow. Further research into behavioural shifts in driving is also recommended due to limited data and knowledge of these dynamics.

  18. Traffic load effects in prestrssed concrete bridges

    NARCIS (Netherlands)

    Vrouwenvelder, A.C.W.M.; Allaix, D.L.; Courage, W.M.G.

    2007-01-01

    The traffic load effect of a two-span prestressed concrete bridge with two traffic lanes is analyzed in this paper. The design value of the result-ing bending moment in some prescribed sections is estimated by means of a Monte Carlo simulation. The model for the vehicular loads is derived from a set

  19. Evaluating Performances of Traffic Noise Models | Oyedepo ...

    African Journals Online (AJOL)

    Traffic noise in decibel dB(A) were measured at six locations using 407780A Integrating Sound Level Meter, while spot speed and traffic volume were collected with cine-camera. The predicted sound exposure level (SEL) was evaluated using Burgess, British and FWHA model. The average noise level obtained are 77.64 ...

  20. Consistency analysis of network traffic repositories

    NARCIS (Netherlands)

    Lastdrager, Elmer; Lastdrager, E.E.H.; Pras, Aiko

    Traffic repositories with TCP/IP header information are very important for network analysis. Researchers often assume that such repositories reliably represent all traffic that has been flowing over the network; little thoughts are made regarding the consistency of these repositories. Still, for

  1. Survivable Impairment-Aware Traffic Grooming

    NARCIS (Netherlands)

    Beshir, A.; Nuijts, R.; Malhotra, R.; Kuipers, F.

    2011-01-01

    Traffic grooming allows efficient utilization of network capacity by aggregating several independent traffic streams into a wavelength. In addition, survivability and impairment-awareness (i.e., taking into account the effect of physical impairments) are two important issues that have gained a lot

  2. Calculating Traffic based on Road Sensor Data

    NARCIS (Netherlands)

    Bisseling, Rob; Gao, Fengnan; Hafkenscheid, Patrick; Idema, Reijer; Jetka, Tomasz; Guerra Ones, Valia; Rata, Debanshu; Sikora, Monika

    2014-01-01

    Road sensors gather a lot of statistical data about traffic. In this paper, we discuss how a measure for the amount of traffic on the roads can be derived from this data, such that the measure is independent of the number and placement of sensors, and the calculations can be performed quickly for

  3. Epidemiology o.f· Traffic Accidents

    African Journals Online (AJOL)

    Accidents. An analysis of some 2 100 fatal traffic accidents gave the following results: males-79%; females-21%; a ratio of 4: 1. The high proportion of males to females killed in traffic accidents may be due to the fact that (a) more males commute daily in private and commercial vehicles;. (b) more females commute daily in ...

  4. Dimensional reduction for conformal blocks

    Science.gov (United States)

    Hogervorst, Matthijs

    2016-09-01

    We consider the dimensional reduction of a CFT, breaking multiplets of the d-dimensional conformal group SO( d + 1 , 1) up into multiplets of SO( d, 1). This leads to an expansion of d-dimensional conformal blocks in terms of blocks in d - 1 dimensions. In particular, we obtain a formula for 3 d conformal blocks as an infinite sum over 2 F 1 hypergeometric functions with closed-form coefficients.

  5. Modeling Network Traffic in Wavelet Domain

    Directory of Open Access Journals (Sweden)

    Sheng Ma

    2004-12-01

    Full Text Available This work discovers that although network traffic has the complicated short- and long-range temporal dependence, the corresponding wavelet coefficients are no longer long-range dependent. Therefore, a "short-range" dependent process can be used to model network traffic in the wavelet domain. Both independent and Markov models are investigated. Theoretical analysis shows that the independent wavelet model is sufficiently accurate in terms of the buffer overflow probability for Fractional Gaussian Noise traffic. Any model, which captures additional correlations in the wavelet domain, only improves the performance marginally. The independent wavelet model is then used as a unified approach to model network traffic including VBR MPEG video and Ethernet data. The computational complexity is O(N for developing such wavelet models and generating synthesized traffic of length N, which is among the lowest attained.

  6. Traffic and Granular Flow ’03

    CERN Document Server

    Luding, Stefan; Bovy, Piet; Schreckenberg, Michael; Wolf, Dietrich

    2005-01-01

    These proceedings are the fifth in the series Traffic and Granular Flow, and we hope they will be as useful a reference as their predecessors. Both the realistic modelling of granular media and traffic flow present important challenges at the borderline between physics and engineering, and enormous progress has been made since 1995, when this series started. Still the research on these topics is thriving, so that this book again contains many new results. Some highlights addressed at this conference were the influence of long range electric and magnetic forces and ambient fluids on granular media, new precise traffic measurements, and experiments on the complex decision making of drivers. No doubt the “hot topics” addressed in granular matter research have diverged from those in traffic since the days when the obvious analogies between traffic jams on highways and dissipative clustering in granular flow intrigued both c- munities alike. However, now just this diversity became a stimulating feature of the ...

  7. A Traffic Restriction Scheme for Enhancing Carpooling

    Directory of Open Access Journals (Sweden)

    Dong Ding

    2017-01-01

    Full Text Available For the purpose of alleviating traffic congestion, this paper proposes a scheme to encourage travelers to carpool by traffic restriction. By a variational inequity we describe travelers’ mode (solo driving and carpooling and route choice under user equilibrium principle in the context of fixed demand and detect the performance of a simple network with various restriction links, restriction proportions, and carpooling costs. Then the optimal traffic restriction scheme aiming at minimal total travel cost is designed through a bilevel program and applied to a Sioux Fall network example with genetic algorithm. According to various requirements, optimal restriction regions and proportions for restricted automobiles are captured. From the results it is found that traffic restriction scheme is possible to enhance carpooling and alleviate congestion. However, higher carpooling demand is not always helpful to the whole network. The topology of network, OD demand, and carpooling cost are included in the factors influencing the performance of the traffic system.

  8. Review on Driverless Traffic from Management Perspective

    Directory of Open Access Journals (Sweden)

    Chen Tingting

    2017-01-01

    Full Text Available The move towards automated driving is gaining ground. This paper reviews the development process of self-driving technology and discusses the safety and efficiency advantages of autonomous vehicles. The discussion shows that the existing traffic management system, including transport infrastructures and regulations, should be changed accordingly to maximize the advantages of autonomous driving. Thus, this paper subsequently gives an insight of the traffic management from three aspects: fully self-driving traffic infrastructures, mixed traffic infrastructures and regulations. First, it is summarized in detail what should be adjusted in intersections, parking lots, pedestrian crossings, ramps, signs and markings. With the transformation of traffic infrastructures, the advantages of driverless car will be more pronounced on account of increased capacity, reduced delay and land use. Also, this paper indicates that the implementations of strict product liability for self-driving car manufacturers and no-fault tort liability for users are applicable to automated vehicle accidents.

  9. Traffic flow dynamics data, models and simulation

    CERN Document Server

    Treiber, Martin

    2013-01-01

    This textbook provides a comprehensive and instructive coverage of vehicular traffic flow dynamics and modeling. It makes this fascinating interdisciplinary topic, which to date was only documented in parts by specialized monographs, accessible to a broad readership. Numerous figures and problems with solutions help the reader to quickly understand and practice the presented concepts. This book is targeted at students of physics and traffic engineering and, more generally, also at students and professionals in computer science, mathematics, and interdisciplinary topics. It also offers material for project work in programming and simulation at college and university level. The main part, after presenting different categories of traffic data, is devoted to a mathematical description of the dynamics of traffic flow, covering macroscopic models which describe traffic in terms of density, as well as microscopic many-particle models in which each particle corresponds to a vehicle and its driver. Focus chapters on ...

  10. Study of air traffic over KLFIR

    Science.gov (United States)

    Nusyirwan, I. F.; Rohani, J. Mohd

    2017-12-01

    This paper shares the overview of the work currently being conducted with the Department of Civil Aviation Malaysia related to the air traffic. The aim is to study air traffic performance over KL and KK FIR, and the area of interest in this paper is the Kuala Lumpur Flight Information Region (KLFIR). The air traffic performance parameters includes general air traffic movement such as level allocation, number of movements, sector load analysis and also more specific parameters such as airborne delays, effects of weather to the air movements as well as ground delays. To achieve this, a huge effort has been undertaken that includes live data collection algorithm development and real time statistical analysis algorithm development. The main outcome from this multi-disciplinary work is the long-term analysis on the air traffic performance in Malaysia, which will put the country at par in the aviation community, namely the International Civil Aviation Organization (ICAO).

  11. Distributed traffic signal control using fuzzy logic

    Science.gov (United States)

    Chiu, Stephen

    1992-01-01

    We present a distributed approach to traffic signal control, where the signal timing parameters at a given intersection are adjusted as functions of the local traffic condition and of the signal timing parameters at adjacent intersections. Thus, the signal timing parameters evolve dynamically using only local information to improve traffic flow. This distributed approach provides for a fault-tolerant, highly responsive traffic management system. The signal timing at an intersection is defined by three parameters: cycle time, phase split, and offset. We use fuzzy decision rules to adjust these three parameters based only on local information. The amount of change in the timing parameters during each cycle is limited to a small fraction of the current parameters to ensure smooth transition. We show the effectiveness of this method through simulation of the traffic flow in a network of controlled intersections.

  12. Learning Potentials in Number Blocks

    DEFF Research Database (Denmark)

    Majgaard, Gunver; Misfeldt, Morten; Nielsen, Jacob

    2012-01-01

    . The tool is called Number Blocks and it combines physical interaction, learning, and immediate feedback. Number Blocks supports the children's understanding of place value in the sense that it allows them to experiment with creating large numbers. We found the blocks contributed to the learning process...... in several ways. The blocks combined mathematics and play, and they included and supported children at different academic levels. The auditory representation, especially the enhanced rhythmic effects due to using speech synthesis, and the rhythm helped the children to pronounce large numbers. This creates...

  13. Common blocks for ASQS(12

    Directory of Open Access Journals (Sweden)

    Lorenzo Milazzo

    1997-05-01

    Full Text Available An ASQS(v is a particular Steiner system featuring a set of v vertices and two separate families of blocks, B and G, whose elements have a respective cardinality of 4 and 6. It has the property that any three vertices of X belong either to a B-block or to a G-block. The parameter cb is the number of common blocks in two separate ASQSs, both defined on the same set of vertices X . In this paper it is shown that cb ≤ 29 for any pair of ASQSs(12.

  14. Peculiarities of Clutch Forming Rails and Wheel Block Construction

    Science.gov (United States)

    Shiler, V. V.; Galiev, I. I.; Shiler, A. V.

    2018-03-01

    The clutch of the wheel and rail is significantly influenced by the design features of the standard wheel pair, which are manifested in the presence of "parasitic" slipping of the wheels along the rails during its movement. The purpose of the presented work is to evaluate new design solutions for wheel sets. The research was carried out using methods of comparative simulation modelling and physical prototyping. A new design of the wheel pair (block wheel pair) is proposed, which features an independent rotation of all surfaces of the wheels in contact with the rails. The block construction of the wheel pair forms open mechanical contours with the track gauge, which completely eliminates the "parasitic" slippage. As a result, in the process of implementing traction or braking forces, the coupling coefficient of the block construction of the wheel pair is significantly higher than that of existing structures. In addition, in the run-out mode, the resistance to movement of the block wheel pair is half as much. All this will allow one to significantly reduce the energy consumption for traction of trains, wear of track elements and crew, and to increase the speed and safety of train traffic.

  15. 31 CFR 545.301 - Blocked account; blocked property.

    Science.gov (United States)

    2010-07-01

    ... (Continued) OFFICE OF FOREIGN ASSETS CONTROL, DEPARTMENT OF THE TREASURY TALIBAN (AFGHANISTAN) SANCTIONS... name of the Taliban or persons whose property or interests in property are blocked pursuant to § 545.201, or in which the Taliban or persons whose property or interests in property are blocked pursuant...

  16. The Stability of Multi-modal Traffic Network

    International Nuclear Information System (INIS)

    Han Linghui; Sun Huijun; Zhu Chengjuan; Jia Bin; Wu Jianjun

    2013-01-01

    There is an explicit and implicit assumption in multimodal traffic equilibrium models, that is, if the equilibrium exists, then it will also occur. The assumption is very idealized; in fact, it may be shown that the quite contrary could happen, because in multimodal traffic network, especially in mixed traffic conditions the interaction among traffic modes is asymmetric and the asymmetric interaction may result in the instability of traffic system. In this paper, to study the stability of multimodal traffic system, we respectively present the travel cost function in mixed traffic conditions and in traffic network with dedicated bus lanes. Based on a day-to-day dynamical model, we study the evolution of daily route choice of travelers in multimodal traffic network using 10000 random initial values for different cases. From the results of simulation, it can be concluded that the asymmetric interaction between the cars and buses in mixed traffic conditions can lead the traffic system to instability when traffic demand is larger. We also study the effect of travelers' perception error on the stability of multimodal traffic network. Although the larger perception error can alleviate the effect of interaction between cars and buses and improve the stability of traffic system in mixed traffic conditions, the traffic system also become instable when the traffic demand is larger than a number. For all cases simulated in this study, with the same parameters, traffic system with dedicated bus lane has better stability for traffic demand than that in mixed traffic conditions. We also find that the network with dedicated bus lane has higher portion of travelers by bus than it of mixed traffic network. So it can be concluded that building dedicated bus lane can improve the stability of traffic system and attract more travelers to choose bus reducing the traffic congestion. (general)

  17. 76 FR 47529 - Port Access Route Study: In the Waters of Montauk Channel and Block Island Sound

    Science.gov (United States)

    2011-08-05

    ... recommended route was subsequently incorporated on nautical charts by the National Oceanic and Atmospheric... traffic patterns in Block Island Sound. No comments were received during the Notice of Study request for... particularly solicited with respect to these recommendations. A navigation chart with the proposed...

  18. Interscalene block for shoulder surgery

    African Journals Online (AJOL)

    2011-06-15

    Jun 15, 2011 ... Fracture dislocation of the shoulder is a common musculoskeletal injury following road traffic accident. Peripheral ... Fracture luxation de l'épaule est une commune blessures musculo-squelettiques suite route trafic accident. Périphériques nerf .... vertebral artery injection, pneumothorax, Horner's syndrome ...

  19. The international traffic in uranium

    International Nuclear Information System (INIS)

    Sharma, S.K.

    1982-01-01

    How the international traffic in uranium, contrary to the spirit of the Non-Proliferation Treaty (NPT), is being carried out by the very nations who are signatories to the NPT is described. The US did not supply enriched uranium for commissioning Koeberg nuclear power plant of South Africa, because South Africa refused to accept full scope safeguards. However, South Africa could procure necessary supplies of enriched uranium, probably from the surplus stocks of enriched uranium of European countries. In contravention of UN sanctions, many European nations are also collaborating with South Africa in illegal mining of uranium in Namibia. All these goings on indicate that the NPT's main objective is not to prevent or reduce proliferation, but to deny nuclear technology to those brown and black nations which do not sign the NPT or do not accept the full scope safeguards. (M.G.B.)

  20. Vision-Based Bicycle Detection Using Multiscale Block Local Binary Pattern

    Directory of Open Access Journals (Sweden)

    Hongyu Hu

    2014-01-01

    Full Text Available Bicycle traffic has heavy proportion among all travel modes in some developing countries, which is crucial for urban traffic control and management as well as facility design. This paper proposes a real-time multiple bicycle detection algorithm based on video. At first, an effective feature called multiscale block local binary pattern (MBLBP is extracted for representing the moving object, which is a well-classified feature to distinguish between bicycles and nonbicycles; then, a cascaded bicycle classifier trained by AdaBoost algorithm is proposed, which has a good computation efficiency. Finally, the method is tested with video sequence captured from the real-world traffic scenario. The bicycles in the test scenario are successfully detected.

  1. Traffic Dynamics of Computer Networks

    Science.gov (United States)

    Fekete, Attila

    2008-10-01

    Two important aspects of the Internet, namely the properties of its topology and the characteristics of its data traffic, have attracted growing attention of the physics community. My thesis has considered problems of both aspects. First I studied the stochastic behavior of TCP, the primary algorithm governing traffic in the current Internet, in an elementary network scenario consisting of a standalone infinite-sized buffer and an access link. The effect of the fast recovery and fast retransmission (FR/FR) algorithms is also considered. I showed that my model can be extended further to involve the effect of link propagation delay, characteristic of WAN. I continued my thesis with the investigation of finite-sized semi-bottleneck buffers, where packets can be dropped not only at the link, but also at the buffer. I demonstrated that the behavior of the system depends only on a certain combination of the parameters. Moreover, an analytic formula was derived that gives the ratio of packet loss rate at the buffer to the total packet loss rate. This formula makes it possible to treat buffer-losses as if they were link-losses. Finally, I studied computer networks from a structural perspective. I demonstrated through fluid simulations that the distribution of resources, specifically the link bandwidth, has a serious impact on the global performance of the network. Then I analyzed the distribution of edge betweenness in a growing scale-free tree under the condition that a local property, the in-degree of the "younger" node of an arbitrary edge, is known in order to find an optimum distribution of link capacity. The derived formula is exact even for finite-sized networks. I also calculated the conditional expectation of edge betweenness, rescaled for infinite networks.

  2. ALCOHOL RELATED TRAFFIC SAFETY LEGISLATION

    Directory of Open Access Journals (Sweden)

    E.B.R. DESAPRIYA

    2002-01-01

    Full Text Available There is a substantial amount of evidence from experimental studies to indicate that a variety of individual skills are impaired at blood alcohol concentrations (BACs well below 0.05%. Epidemiological studies indicate that the risk of a crash increases sharply for drivers with BACs below 0.05%. The correlation between drunk driving and the risk of traffic accidents has been established on the individual as well as the aggregate level. The BAC level legally permitted is a public policy decision by legislators, while scientists can present experimental and epidemiological evidence indicating the BAC level at which psychomotor skills deteriorate and accident probabilities increase. There is considerable epidemiological evidence to support the fact that the risk of alcohol impaired drivers being involved in traffic crashes rises with increasing BAC's. By contrast, the evidence on the BAC at which a driver should be regarded as committing an offence has been the subject of much debate and various legislative decisions. Historically, per se laws specify BAC levels which are a compromise figure intended to reflect both the point at which a driver becomes significantly more likely to be involved in an accident than a comparative driver with a zero BAC and that which is politically acceptable, but falls within the BAC region of increased accident liability. Therefore, the per se legislation in most countries has not kept pace with scientific progress. This study suggests that if saving lives on the road is an important issue, then, passing laws that incorporate scientific and epidemiological studies, is necessary.

  3. MOBILIDADE URBANA E TRAFFIC CALMING

    Directory of Open Access Journals (Sweden)

    Priscilla Alves

    2014-09-01

    Full Text Available O objetivo do presente trabalho é o de analisar e compreender, a partir de uma discussão teórica, o conceito e aplicação das medidas moderadoras de tráfego, conhecidas como traffic calming e sua relação com a mobilidade urbana sustentável. Buscou-se conhecer a aplicação da técnica no cenário mundial assim como algumas medidas adotadas no Brasil. Os procedimentos metodológicos aqui utilizados envolvem uma pesquisa exploratória, através da literatura atual e disponível, acerca dos temas mobilidade urbana sustentável e aplicação das medidas traffic calming. Conclui-se que as medidas de moderação atuam em conformidade com os princípios da mobilidade urbana sustentável na medida em que prioriza ações para redução do tráfego motorizado, e incentiva o uso e a segurança nos deslocamentos não motorizados. Contudo, a aplicação das técnicas sem estudos prévios e um planejamento estratégico não reduz os conflitos de mobilidade existentes nas localidades. No caso do Brasil para que se tenha sucesso com implantação da técnica é preciso uma análise mais abrangente, articulada e ser passível de aplicação de forma completa, assim como ocorre nos países desenvolvidos.

  4. Classical Virasoro irregular conformal block

    Science.gov (United States)

    Rim, Chaiho; Zhang, Hong

    2015-07-01

    Virasoro irregular conformal block with arbitrary rank is obtained for the classical limit or equivalently Nekrasov-Shatashvili limit using the beta-deformed irregular matrix model (Penner-type matrix model for the irregular conformal block). The same result is derived using the generalized Mathieu equation which is equivalent to the loop equation of the irregular matrix model.

  5. Classical Virasoro irregular conformal block

    Energy Technology Data Exchange (ETDEWEB)

    Rim, Chaiho; Zhang, Hong [Department of Physics and Center for Quantum Spacetime (CQUeST), Sogang University,Seoul 121-742 (Korea, Republic of)

    2015-07-30

    Virasoro irregular conformal block with arbitrary rank is obtained for the classical limit or equivalently Nekrasov-Shatashvili limit using the beta-deformed irregular matrix model (Penner-type matrix model for the irregular conformal block). The same result is derived using the generalized Mathieu equation which is equivalent to the loop equation of the irregular matrix model.

  6. Four-block beam collimator

    CERN Document Server

    CERN PhotoLab

    1977-01-01

    The photo shows a four-block collimator installed on a control table for positioning the alignment reference marks. Designed for use with the secondary beams, the collimators operated in vacuum conditions. The blocks were made of steel and had a standard length of 1 m. The maximum aperture had a square coss-section of 144 cm2. (See Annual Report 1976.)

  7. OPAL Various Lead Glass Blocks

    CERN Document Server

    These lead glass blocks were part of a CERN detector called OPAL (one of the four experiments at the LEP particle detector). OPAL uses some 12 000 blocks of glass like this to measure particle energies in the electromagnetic calorimeter. This detector measured the energy deposited when electrons and photons were slowed down and stopped.

  8. Writing Blocks and Tacit Knowledge.

    Science.gov (United States)

    Boice, Robert

    1993-01-01

    A review of the literature on writing block looks at two kinds: inability to write in a timely, fluent fashion, and reluctance by academicians to assist others in writing. Obstacles to fluent writing are outlined, four historical trends in treating blocks are discussed, and implications are examined. (MSE)

  9. Block storage subsystem performance analysis

    CERN Multimedia

    CERN. Geneva

    2016-01-01

    You feel that your service is slow because of the storage subsystem? But there are too many abstraction layers between your software and the raw block device for you to debug all this pile... Let's dive on the platters and check out how the block storage sees your I/Os! We can even figure out what those patterns are meaning.

  10. Research on the Method of Traffic Organization and Optimization Based on Dynamic Traffic Flow Model

    Directory of Open Access Journals (Sweden)

    Shu-bin Li

    2017-01-01

    Full Text Available The modern transportation system is becoming sluggish by traffic jams, so much so that it can harm the economic and society in our country. One of the reasons is the surging vehicles day by day. Another reason is the shortage of the traffic supply seriously. But the most important reason is that the traffic organization and optimization hardly met the conditions of modern transport development. In this paper, the practical method of the traffic organization and optimization used in regional area is explored by the dynamic traffic network analysis method. Firstly, the operational states of the regional traffic network are obtained by simulation method based on the self-developed traffic simulation software DynaCHINA, in which the improved traffic flow simulation model was proposed in order to be more suitable for actual domestic urban transport situation. Then the appropriated optimization model and algorithm were proposed according to different optimized content and organization goals, and the traffic simulation processes more suitable to regional optimization were designed exactly. Finally, a regional network in Tai’an city was selected as an example. The simulation results show that the proposed method is effective and feasible. It can provide strong scientific and technological support for the traffic management department.

  11. A new inhibitor of the β-arrestin/AP2 endocytic complex reveals interplay between GPCR internalization and signalling

    Science.gov (United States)

    Beautrait, Alexandre; Paradis, Justine S.; Zimmerman, Brandon; Giubilaro, Jenna; Nikolajev, Ljiljana; Armando, Sylvain; Kobayashi, Hiroyuki; Yamani, Lama; Namkung, Yoon; Heydenreich, Franziska M.; Khoury, Etienne; Audet, Martin; Roux, Philippe P.; Veprintsev, Dmitry B.; Laporte, Stéphane A.; Bouvier, Michel

    2017-04-01

    In addition to G protein-coupled receptor (GPCR) desensitization and endocytosis, β-arrestin recruitment to ligand-stimulated GPCRs promotes non-canonical signalling cascades. Distinguishing the respective contributions of β-arrestin recruitment to the receptor and β-arrestin-promoted endocytosis in propagating receptor signalling has been limited by the lack of selective analytical tools. Here, using a combination of virtual screening and cell-based assays, we have identified a small molecule that selectively inhibits the interaction between β-arrestin and the β2-adaptin subunit of the clathrin adaptor protein AP2 without interfering with the formation of receptor/β-arrestin complexes. This selective β-arrestin/β2-adaptin inhibitor (Barbadin) blocks agonist-promoted endocytosis of the prototypical β2-adrenergic (β2AR), V2-vasopressin (V2R) and angiotensin-II type-1 (AT1R) receptors, but does not affect β-arrestin-independent (transferrin) or AP2-independent (endothelin-A) receptor internalization. Interestingly, Barbadin fully blocks V2R-stimulated ERK1/2 activation and blunts cAMP accumulation promoted by both V2R and β2AR, supporting the concept of β-arrestin/AP2-dependent signalling for both G protein-dependent and -independent pathways.

  12. Remotely Accessed Vehicle Traffic Management System

    Science.gov (United States)

    Al-Alawi, Raida

    2010-06-01

    The ever increasing number of vehicles in most metropolitan cities around the world and the limitation in altering the transportation infrastructure, led to serious traffic congestion and an increase in the travelling time. In this work we exploit the emergence of novel technologies such as the internet, to design an intelligent Traffic Management System (TMS) that can remotely monitor and control a network of traffic light controllers located at different sites. The system is based on utilizing Embedded Web Servers (EWS) technology to design a web-based TMS. The EWS located at each intersection uses IP technology for communicating remotely with a Central Traffic Management Unit (CTMU) located at the traffic department authority. Friendly GUI software installed at the CTMU will be able to monitor the sequence of operation of the traffic lights and the presence of traffic at each intersection as well as remotely controlling the operation of the signals. The system has been validated by constructing a prototype that resembles the real application.

  13. A First Look at Modern Enterprise Traffic

    Energy Technology Data Exchange (ETDEWEB)

    Pang, Ruoming; Mark Allman, Mark; Bennett, Mike; Lee, Jason; Paxson, Vern; Tierney, Brian

    2005-06-01

    While wide-area Internet traffic has been heavily studied for many years, the characteristics of traffic inside Internet enterprises remain almost wholly unexplored. Nearly all of the studies of enterprise traffic available in the literature are well over a decade old and focus on individual LANs rather than whole sites. In this paper we present a broad overview of internal enterprise traffic recorded at a medium-sized site. The packet traces span more than 100 hours, over which activity from a total of several thousand internal hosts appears. This wealth of data--which we are publicly releasing in anonymized form--spans a wide range of dimensions. While we cannot form general conclusions using data from a single site, and clearly this sort of data merits additional in-depth study in a number of ways, in this work we endeavor to characterize a number of the most salient aspects of the traffic. Our goal is to provide a first sense of ways in which modern enterprise traffic is similar to wide-area Internet traffic, and ways in which it is quite different.

  14. A Signal Coordination Control Based on Traversing Empty between Mid-Block Street Crossing and Intersection

    Directory of Open Access Journals (Sweden)

    Changjiang Zheng

    2012-01-01

    Full Text Available To solve the problem in pedestrian Mid-Block street crossing, the method of signal coordination control between mid-block street crossing and intersection is researched in this paper. The paper proposes to use “distance-flow rate-time” graph as the tool for building coordination control system model which is for different situations of traffic control. Through alternating the linear optimization model, the system outputs the distribution of signal timing and system operational factors (delays in vehicles and mid-block street crossing. Finally, taking one section on the Taiping North Road in Nanjing as an example, the signal coordination control is carried out. And the results which are delays in the vehicles and mid-block street crossing are compared to those in the current distribution of signal timing.

  15. Validating the passenger traffic model for Copenhagen

    DEFF Research Database (Denmark)

    Overgård, Christian Hansen; VUK, Goran

    2006-01-01

    The paper presents a comprehensive validation procedure for the passenger traffic model for Copenhagen based on external data from the Danish national travel survey and traffic counts. The model was validated for the years 2000 to 2004, with 2004 being of particular interest because the Copenhagen...... matched the observed traffic better than those of the transit assignment model. With respect to the metro forecasts, the model over-predicts metro passenger flows by 10% to 50%. The wide range of findings from the project resulted in two actions. First, a project was started in January 2005 to upgrade...

  16. Modelling Traffic Flows Using Graph Theory

    Directory of Open Access Journals (Sweden)

    Oksana Musyt

    2011-04-01

    Full Text Available An intensive increase in road transport, particularly individual, in recent years has led to such consequences as increased time spent on travel, the number of forced stops, traffic accidents, the occurrence of traffic jams on the road network, reducing traffic speed and a deteriorated urban road network in cities. The most effective method for solving these problems is the use of graph theory, the main characteristics of which is reliability, durability and accessibility of a free as well as loaded network. Based on their analysis the methods for network optimization are proposed.Article in Russian

  17. Stationarity Testing of Accumulated Ethernet Traffic

    Directory of Open Access Journals (Sweden)

    Zhiping Lu

    2013-01-01

    Full Text Available We investigate the stationarity property of the accumulated Ethernet traffic series. We applied several widely used stationarity and unit root tests, such as Dickey-Fuller test and its augmented version, Phillips-Perron test, as well as the Kwiatkowski-Phillips-Schmidt-Shin test and some of its generalizations, to the assessment of the stationarity of the traffic traces at the different time scales. The quantitative results in this research provide evidence that when the time scale increases, the accumulated traffic series are more stationary.

  18. Elevator traffic handbook theory and practice

    CERN Document Server

    Barney, Gina

    2015-01-01

    This second edition of this well-respected book covers all aspects of the traffic design and control of vertical transportation systems in buildings, making it an essential reference for vertical transportation engineers, other members of the design team, and researchers. The book introduces the basic principles of circulation, outlines traffic design methods and examines and analyses traffic control using worked examples and case studies to illustrate key points. The latest analysis techniques are set out, and the book is up-to-date with current technology. A unique and well-established book, this much-needed new edition features extensive updates to technology and practice, drawing on the latest international research.

  19. International Workshop on Traffic and Granular Flow

    CERN Document Server

    Herrmann, Hans; Schreckenberg, Michael; Wolf, Dietrich; Social, Traffic and Granular Dynamics

    2000-01-01

    "Are there common phenomena and laws in the dynamic behavior of granular materials, traffic, and socio-economic systems?" The answers given at the international workshop "Traffic and Granular Flow '99" are presented in this volume. From a physical standpoint, all these systems can be treated as (self)-driven many-particle systems with strong fluctuations, showing multistability, phase transitions, non-linear waves, etc. The great interest in these systems is due to several unexpected new discoveries and their practical relevance for solving some fundamental problems of today's societies. This includes intelligent measures for traffic flow optimization and methods from "econophysics" for stabilizing (stock) markets.

  20. Strategic Framework of Air Traffic Development

    Directory of Open Access Journals (Sweden)

    Sanja Steiner

    2008-05-01

    Full Text Available The paper elaborates the past development of air traffic inEurope from the aspect of its economic benefits and ecologicallack of sustainability of conventional technology. The mainguidelines of the strategic development are related to the implementationof the global ecological standard and actual reformationprocesses of the air traffic management system postulatedby A TM 2000+ Strategy and the "Single European Sky"standard. The paper studies the wider aspects of integrated trafficdevelopment at the regional level and the factors influencingfurther development of the European air traffic.

  1. Pro CSS for High Traffic Websites

    CERN Document Server

    Kennedy, A

    2011-01-01

    Although web standards-based websites can scale effectively - and basic CSS will give you basic results - there are considerations and obstacles that high traffic websites must face to keep your development and hosting costs to a minimum. There are many tips and tricks, as well as down-to-earth best practice information, to make sure that everything runs quickly and efficiently with the minimum amount of fuss or developer intervention. Targeted at "high traffic" websites - those receiving over 10,000 unique visitors a day - Pro CSS for High Traffic Websites gives you inside informati

  2. Stochastic Modeling of Traffic Air Pollution

    DEFF Research Database (Denmark)

    Thoft-Christensen, Palle

    2014-01-01

    In this paper, modeling of traffic air pollution is discussed with special reference to infrastructures. A number of subjects related to health effects of air pollution and the different types of pollutants are briefly presented. A simple model for estimating the social cost of traffic related air...... and using simple Monte Carlo techniques to obtain a stochastic estimate of the costs of traffic air pollution for infrastructures....... pollution is derived. Several authors have published papers on this very complicated subject, but no stochastic modelling procedure have obtained general acceptance. The subject is discussed basis of a deterministic model. However, it is straightforward to modify this model to include uncertain parameters...

  3. Graph Wavelets for Spatial Traffic Analysis

    Science.gov (United States)

    2002-07-15

    Proceedings of ACM SIGCOMM ’95, 1995, pp. 100–113. [5] J. Levy-Vehel and R. Riedi, “Fractional Brownian motion and data traffic modeling: the other end of...similarity and long-range dependence, [3, 4]; and multifractality and local scaling [5, 6]. Many of the key results in traffic time scaling analysis have...as cascades: Investigating the multifractal nature of Internet WAN traffic,” in Proceedings of SIGCOMM ’98, October 1998, pp. 42–55. [7] P. Abry and

  4. Green Wave Traffic Optimization - A Survey

    DEFF Research Database (Denmark)

    Warberg, Andreas; Larsen, Jesper; Jørgensen, Rene Munk

    The objective of this survey is to cover the research in the area of adaptive traffic control with emphasis on the applied optimization methods. The problem of optimizing traffic signals can be viewed in various ways, depending on political, economic and ecological goals. The survey highlights some...... important conflicts, which support the notion that traffic signal optimization is a multi-objective problem, and relates this to the most common measures of effectiveness. A distinction can be made between classical systems, which operate with a common cycle time, and the more flexible, phase...

  5. Criminal Justice Systems. Block I: Law Enforcement. Block II: The Courts. Block III: Corrections. Block IV: Community Relations. Block V: Proficiency Skills. Block VI: Criminalistics. Student Guide.

    Science.gov (United States)

    Florida State Dept. of Education, Tallahassee. Div. of Vocational, Adult, and Community Education.

    This student guide together with an instructor guide comprise a set of curriculum materials on the criminal justice system. The student guide contains self-contained instructional material that students can study at their own pace most of the time. Six major subject areas or blocks, which are further broken down into several units, with some units…

  6. Criminal Justice Systems. Block I: Law Enforcement. Block II: The Courts. Block III: Corrections. Block IV: Community Relations. Block V: Proficiency Skills. Block VI: Criminalistics. Instructor Guide.

    Science.gov (United States)

    Florida State Dept. of Education, Tallahassee. Div. of Vocational, Adult, and Community Education.

    This instructor guide together with a student guide comprise a set of curriculum materials on the criminal justice system. The instructor guide is a resource for planning and managing individualized, competency-based instruction in six major subject areas or blocks, which are further broken down into several units with some units having several…

  7. Traffic jam induced by a crosscut road in a traffic-flow model

    Science.gov (United States)

    Nagatani, Takashi; Seno, Tadachika

    1994-06-01

    A deterministic cellular automaton model is presented to simulate the traffic jam induced by a crosscut road in a two-dimensional traffic flow. The effect of a crosscut road on the traffic flow is investigated by the use of a computer simulation. The traffic jam appears when a shock (discontinuous interface of different car densities) is formed. The condition for shock formation is derived for car densities p y and p x of the crosscut road and its crossing streets. The phase diagram and the dependence of the traffic flow on the car densities are shown. Also, we study the shock structure and the scaling of its width. The width Δ w of the shock scales with the system size L as Δ w ≈ L{1}/{2}. We present a self-consistent mean-field theory for the traffic flow.

  8. Calculating Distribution and Intensity of Agricultural Traffic for Sustainable Development

    NARCIS (Netherlands)

    Hospers, A.; Louwsma, M.A.; Lammeren, van R.J.A.; Kuiper, P.P.

    2016-01-01

    Land from many holdings is dispersed, which compels farmers to use public roads to access distant parcels. Agricultural traffic is different from other traffic with its large and heavy vehicles. When agricultural traffic mixes with other traffic, safety issues may arise. Another issue of

  9. Design and Development of Biometric Traffic Offence System (BTOS ...

    African Journals Online (AJOL)

    The study was about the design and development of Biometric Traffic Offence System (BTOS) that would ease storage and retrieval of traffic offence cases in the Federal Road Safety Commission (FRSC) and the Traffic department of Nigeria Police force. The method used to manage traffic cases is manual; information ...

  10. Traffic Driven Analysis of Cellular and WiFi Networks

    Science.gov (United States)

    Paul, Utpal Kumar

    2012-01-01

    Since the days Internet traffic proliferated, measurement, monitoring and analysis of network traffic have been critical to not only the basic understanding of large networks, but also to seek improvements in resource management, traffic engineering and security. At the current times traffic in wireless local and wide area networks are facing…

  11. Linking network usage patterns to traffic Gaussianity fit

    NARCIS (Netherlands)

    de Oliveira Schmidt, R.; Sadre, R.; Melnikov, Nikolay; Schönwälder, Jürgen; Pras, Aiko

    Gaussian traffic models are widely used in the domain of network traffic modeling. The central assumption is that traffic aggregates are Gaussian distributed. Due to its importance, the Gaussian character of network traffic has been extensively assessed by researchers in the past years. In 2001,

  12. 32 CFR 634.32 - Traffic violation reports.

    Science.gov (United States)

    2010-07-01

    ... 32 National Defense 4 2010-07-01 2010-07-01 true Traffic violation reports. 634.32 Section 634.32 National Defense Department of Defense (Continued) DEPARTMENT OF THE ARMY (CONTINUED) LAW ENFORCEMENT AND CRIMINAL INVESTIGATIONS MOTOR VEHICLE TRAFFIC SUPERVISION Traffic Supervision § 634.32 Traffic violation...

  13. 32 CFR 634.28 - Traffic accident investigation.

    Science.gov (United States)

    2010-07-01

    ... 32 National Defense 4 2010-07-01 2010-07-01 true Traffic accident investigation. 634.28 Section 634.28 National Defense Department of Defense (Continued) DEPARTMENT OF THE ARMY (CONTINUED) LAW ENFORCEMENT AND CRIMINAL INVESTIGATIONS MOTOR VEHICLE TRAFFIC SUPERVISION Traffic Supervision § 634.28 Traffic...

  14. 32 CFR 634.25 - Installation traffic codes.

    Science.gov (United States)

    2010-07-01

    ... 32 National Defense 4 2010-07-01 2010-07-01 true Installation traffic codes. 634.25 Section 634.25 National Defense Department of Defense (Continued) DEPARTMENT OF THE ARMY (CONTINUED) LAW ENFORCEMENT AND CRIMINAL INVESTIGATIONS MOTOR VEHICLE TRAFFIC SUPERVISION Traffic Supervision § 634.25 Installation traffic...

  15. 32 CFR 634.29 - Traffic accident investigation reports.

    Science.gov (United States)

    2010-07-01

    ... 32 National Defense 4 2010-07-01 2010-07-01 true Traffic accident investigation reports. 634.29 Section 634.29 National Defense Department of Defense (Continued) DEPARTMENT OF THE ARMY (CONTINUED) LAW ENFORCEMENT AND CRIMINAL INVESTIGATIONS MOTOR VEHICLE TRAFFIC SUPERVISION Traffic Supervision § 634.29 Traffic...

  16. Traffic Measurement and Timestamp Accuracy Evaluation of Java ME Traffic Generator on Smartphone

    OpenAIRE

    Huq, Mohammed Azizul

    2011-01-01

    Research on network traffic measurement is becoming popular because it could find the reason and valuable information regarding the occurrence of timestamp accuracy error. In this thesis the performance of UDP traffic between application level and link level has been investigated on three different Smartphone operating systems. One Java ME traffic generator and sink was developed for this thesis work. Distributed Passive Measurement Infrastructure with Measurement Point was used for the exper...

  17. Block by Block: Civic Action in the Battle of Baghdad

    Science.gov (United States)

    2007-11-01

    bedding, and latrine facilities. Additionally, provide milk , baby formula, diapers, 7 Bogart: Block by Block and infant/family care items such as...viable agricultural businesses. The cattle are a cross breed of a “regular” Iraqi cow and a water buffalo. Chicken farms are mostly egg farms, and...a problem. Contractors did not want to work for fear of being shot or kid - napped. For example, four contractors were shot over the duration of

  18. Harmony of spinning conformal blocks

    Energy Technology Data Exchange (ETDEWEB)

    Schomerus, Volker [DESY Hamburg, Theory Group,Notkestraße 85, 22607 Hamburg (Germany); Sobko, Evgeny [Nordita and Stockholm University,Roslagstullsbacken 23, SE-106 91 Stockholm (Sweden); Isachenkov, Mikhail [Department of Particle Physics and Astrophysics, Weizmann Institute of Science,Rehovot 7610001 (Israel)

    2017-03-15

    Conformal blocks for correlation functions of tensor operators play an increasingly important role for the conformal bootstrap programme. We develop a universal approach to such spinning blocks through the harmonic analysis of certain bundles over a coset of the conformal group. The resulting Casimir equations are given by a matrix version of the Calogero-Sutherland Hamiltonian that describes the scattering of interacting spinning particles in a 1-dimensional external potential. The approach is illustrated in several examples including fermionic seed blocks in 3D CFT where they take a very simple form.

  19. Harmony of spinning conformal blocks

    Energy Technology Data Exchange (ETDEWEB)

    Schomerus, Volker [Deutsches Elektronen-Synchrotron (DESY), Hamburg (Germany). Theory Group; Sobko, Evgeny [Stockholm Univ. (Sweden); Nordita, Stockholm (Sweden); Isachenkov, Mikhail [Weizmann Institute of Science, Rehovoth (Israel). Dept. of Particle Physics and Astrophysics

    2016-12-07

    Conformal blocks for correlation functions of tensor operators play an increasingly important role for the conformal bootstrap programme. We develop a universal approach to such spinning blocks through the harmonic analysis of certain bundles over a coset of the conformal group. The resulting Casimir equations are given by a matrix version of the Calogero-Sutherland Hamiltonian that describes the scattering of interacting spinning particles in a 1-dimensional external potential. The approach is illustrated in several examples including fermionic seed blocks in 3D CFT where they take a very simple form.

  20. Ventilation and Air Quality in City Blocks Using Large-Eddy Simulation—Urban Planning Perspective

    Directory of Open Access Journals (Sweden)

    Mona Kurppa

    2018-02-01

    Full Text Available Buildings and vegetation alter the wind and pollutant transport in urban environments. This comparative study investigates the role of orientation and shape of perimeter blocks on the dispersion and ventilation of traffic-related air pollutants, and the street-level concentrations along a planned city boulevard. A large-eddy simulation (LES model PALM is employed over a highly detailed representation of the urban domain including street trees and forested areas. Air pollutants are represented by massless and passive particles (non-reactive gases, which are released with traffic-related emission rates. High-resolution simulations for four different city-block-structures are conducted over a 8.2 km 2 domain under two contrasting inflow conditions with neutral and stable atmospheric stratification corresponding the general and wintry meteorological conditions. Variation in building height together with multiple cross streets along the boulevard improves ventilation, resulting in 7–9% lower mean concentrations at pedestrian level. The impact of smaller scale variability in building shape was negligible. Street trees further complicate the flow and dispersion. Notwithstanding the surface roughness, atmospheric stability controls the concentration levels with higher values under stably stratified inflow. Little traffic emissions are transported to courtyards. The results provide urban planners direct information to reduce air pollution by proper structural layout of perimeter blocks.

  1. Integrated corridor traffic management : final evaluation report

    Science.gov (United States)

    2000-04-01

    In 1993, the Minnesota Department of Transportation (Mn/DOT) forged a partnership with Hennepin County and the cities of Bloomington Richfield, and Edina to test the concept of integrated corridor traffic management (ICTM) across jurisdictional bound...

  2. Integrated Corridor Traffic Management Evaluation Report

    Science.gov (United States)

    2000-04-01

    In 1993, the Minnesota Department of Transportation (Mn/DOT) forged a partnership with Hennepin County and the cities of Bloomington Richfield, and Edina to test the concept of integrated corridor traffic management (ICTM) across jurisdictional bound...

  3. Training for healthy older drivers : traffic tech.

    Science.gov (United States)

    2013-05-01

    The research described in this edition of "Traffic Tech" examined the effectiveness of four types of : training techniques designed to improve the driving performance : of normally aging adults. Each technique is suitable : for a broad cross-section ...

  4. Beyond traffic : the smart city challenge.

    Science.gov (United States)

    2016-01-01

    The U.S. Department of Transportation (USDOT) Beyond Traffic 2045: Trends and Choices : study indicates that many communities will experience rapid population increases and : rapidly growing demands on their transportation infrastructure in the next ...

  5. Pittsburgh International Airport - Scheduled Passenger Traffic

    Data.gov (United States)

    Allegheny County / City of Pittsburgh / Western PA Regional Data Center — Attached is Pittsburgh International Airport’s Monthly Scheduled Traffic Report. This report contains a year-over-year (YOY) analysis of scheduled flights and...

  6. Transverse speed bars for rural traffic calming.

    Science.gov (United States)

    2013-10-01

    Small rural communities often lack the expertise and resources necessary to address speeding and the persistent challenge of slowing high-speed through traffic. The entrances to communities are especially problematic given that drivers must transitio...

  7. Traffic Predictive Control: Case Study and Evaluation

    Science.gov (United States)

    2017-06-26

    This project developed a quantile regression method for predicting future traffic flow at a signalized intersection by combining both historical and real-time data. The algorithm exploits nonlinear correlations in historical measurements and efficien...

  8. Developments in weather responsive traffic management strategies.

    Science.gov (United States)

    2011-06-01

    This report provides a comprehensive overview of weather-responsive traffic management practices. It focuses on what WRTM : strategies exist, where they have been used, the benefits realized, what improvements are needed, and how to implement and : e...

  9. ROAD TRAFFIC ESTIMATION USING BLUETOOTH SENSORS

    Directory of Open Access Journals (Sweden)

    Monika N. BUGDOL

    2017-09-01

    Full Text Available The Bluetooth standard is a low-cost, very popular communication protocol offering a wide range of applications in many fields. In this paper, a novel system for road traffic estimation using Bluetooth sensors has been presented. The system consists of three main modules: filtration, statistical analysis of historical, and traffic estimation and prediction. The filtration module is responsible for the classification of road users and detecting measurements that should be removed. Traffic estimation has been performed on the basis of the data collected by Bluetooth measuring devices and information on external conditions (e.g., temperature, all of which have been gathered in the city of Bielsko-Biala (Poland. The obtained results are very promising. The smallest average relative error between the number of cars estimated by the model and the actual traffic was less than 10%.

  10. 2004 road traffic crashes in Queensland

    Science.gov (United States)

    2009-05-01

    This report presents an overview of reported road traffic crashes in Queensland during : 2004 in the context of the previous five years based on data contained in the Queensland : Road Crash Information System maintained by the Department of Transpor...

  11. Louisiana traffic sign inventory and management system.

    Science.gov (United States)

    2003-09-01

    The Louisiana Department of Transportation and Development (DOTD), like many state highway agencies in the United States, lacks a comprehensive system for inventorying and maintaining records of traffic signs. To address this problem, state highway d...

  12. Video Traffic Analysis for Abnormal Event Detection

    Science.gov (United States)

    2010-01-01

    We propose the use of video imaging sensors for the detection and classification of abnormal events to be used primarily for mitigation of traffic congestion. Successful detection of such events will allow for new road guidelines; for rapid deploymen...

  13. LED traffic signal management system : final report.

    Science.gov (United States)

    2016-06-01

    This research originated from the opportunity to develop a methodology to assess when LED (Light Emitting Diode) traffic signal modules begin to fail to meet the Institute of Transportation Engineers (ITE) performance specification for luminous inten...

  14. Fuzzy logic controller for a traffic signal

    Science.gov (United States)

    Janecek, J. J.; Zargham, Mehdi R.

    1995-08-01

    Automatic control of traffic signals in many cities are often based on a constant green-to-red cycle. The time period for green light (or red light) to be on is fixed, and is determined based on a stochastic model. In some situations, a human operator, or a fixed timer, may change the time period dyring time intervals where there is heavy traffic--typically during commuting times. Although in today's traffic controllers, the time period for green light can be changed in certain circumstances, in general they are not able to adjust dynamically to traffic changes. This paper presents the design of a controller that considers the intersection of two two-way streets and is able to adjust to environmental changes. Simulation of the proposed controller has produced results more in line with what we expected. In many cases, the proposed system has produced better results than systems that are based on a constant green-to-red cycle.

  15. U18 : Traffic signal safety (phase B).

    Science.gov (United States)

    2009-08-01

    Efficiently scheduling traffic, particularly heavy vehicles, remains a key challenge in transportation engineering. This project has focused on the development of a novel trafficsignal-control methodology to improve the safety of heavy vehicles on...

  16. Traffic control device evaluation program : FY 2016.

    Science.gov (United States)

    2017-03-01

    This report presents findings on three different activities conducted in the Traffic Control Device Evaluation Program during the 2016 fiscal year. The first two activities are evaluations of full-matrix color light-emitting diode changeable message ...

  17. Caltrans Average Annual Daily Traffic Volumes (2004)

    Data.gov (United States)

    California Environmental Health Tracking Program — [ from http://www.ehib.org/cma/topic.jsp?topic_key=79 ] Traffic exhaust pollutants include compounds such as carbon monoxide, nitrogen oxides, particulates (fine...

  18. Railway Network Timetabling and Dynamic Traffic Management

    NARCIS (Netherlands)

    Hansen, I.A.

    2009-01-01

    The paper discusses the current state of research concerning railway network timetabling and traffic management. Timetable effectiveness is governed by frequency, regularity, accurate running, recovery and layover times, as well as minimal headway, buffer times and waiting times. Analytic (queuing)

  19. Information sharing for traffic incident management.

    Science.gov (United States)

    2009-01-01

    Traffic incident management focuses on developing procedures, implementing policies, and deploying technologies to more quickly identify incidents, improve response times, and more effectively and efficiently manage the incident scene. Because so man...

  20. LaGuardia air traffic control tower.

    Science.gov (United States)

    2011-01-01

    To celebrate FAA and its LaGuardia Airport employees past, : present, and future this booklet outlines the airports history and accomplishments and includes copies of some of the photographs in the : air traffic control towers history g...

  1. Management of Traffic Congestion in Urban Areas

    Directory of Open Access Journals (Sweden)

    Vilibald Premzl

    2012-10-01

    Full Text Available The use of road vehicles is increasing, the benefits they affordhave been progressively diminished by external costs.Whereas traffic increases as we approach the centre, the roadand open space decreases. The greater specialisation allows thecity growth in size and in traffic attraction. In this way urbangrowth feeds itself !mer-urban transp011 facilities also becomemore extensive. Growth in size of the city generates greateramounts of traffic and can eventually give rise to agglomerationdiseconomies. Higher transport costs, offices and shops, attractedby the accessibility of central locations, gradually replaceresidential uses, people being forced to seek housing inthe suburbs. As the urban area expands and offices in the citycentre are built denser and highe1; traffic congestion increases.This may result in the fall in centra/land values, since accessibilitydiminishes with the saturation of transport network. Increasedpollution takes various forms as noise, smoke andovercrowded housing in the centre, urban decay in the transitionalzone as commercial development is anticipated.

  2. Video traffic analysis for abnormal event detection.

    Science.gov (United States)

    2010-01-01

    We propose the use of video imaging sensors for the detection and classification of abnormal events to : be used primarily for mitigation of traffic congestion. Successful detection of such events will allow for : new road guidelines; for rapid deplo...

  3. Improved Channel Allocation and RLC block scheduling for Downlink traffic in GPRS

    DEFF Research Database (Denmark)

    Wang, Haibo; Devendra, Devendra; Zhou, Xin

    This paper investigates the Radio Resource Management (RRM) approaches for GSM/GPRS networks. The effort was put on Access Control(AC), Time-Slot (TS) allocation, and Radio Link Control (RLC) packet scheduling for down-link performance enhancement. A new credit-based algorithm was proposed...

  4. Macroscopic Traffic State Estimation: Understanding Traffic Sensing Data-Based Estimation Errors

    Directory of Open Access Journals (Sweden)

    Paul B. C. van Erp

    2017-01-01

    Full Text Available Traffic state estimation is a crucial element in traffic management systems and in providing traffic information to road users. In this article, we evaluate traffic sensing data-based estimation error characteristics in macroscopic traffic state estimation. We consider two types of sensing data, that is, loop-detector data and probe speed data. These data are used to estimate the mean speed in a discrete space-time mesh. We assume that there are no errors in the sensing data. This allows us to study the errors resulting from the differences in characteristics between the sensing data and desired estimate together with the incomplete description of the relation between the two. The aim of the study is to evaluate the dependency of this estimation error on the traffic conditions and sensing data characteristics. For this purpose, we use microscopic traffic simulation, where we compare the estimates with the ground truth using Edie’s definitions. The study exposes a relation between the error distribution characteristics and traffic conditions. Furthermore, we find that it is important to account for the correlation between individual probe data-based estimation errors. Knowledge related to these estimation errors contributes to making better use of the available sensing data in traffic state estimation.

  5. Is it traffic type, volume, or distance? Wheezing in infants living near truck and bus traffic.

    Science.gov (United States)

    Ryan, Patrick H; LeMasters, Grace; Biagini, Jocelyn; Bernstein, David; Grinshpun, Sergey A; Shukla, Rakesh; Wilson, Kimberly; Villareal, Manuel; Burkle, Jeff; Lockey, James

    2005-08-01

    Previous studies of air pollution have not examined the association between exposure to varying types, distance, and amounts of traffic and wheezing in very young infants. We sought to determine the relationship between types of traffic, traffic volume, and distance and wheezing among infants less than 1 year of age. A geographic information system and a classification scheme were developed to categorize infants enrolled in the study as living near moving truck and bus traffic (highway >50 miles per hour, >1000 trucks daily, bus traffic (bus and truck traffic had a significantly increased prevalence of wheezing (adjusted odds ratio, 2.50; 95% CI, 1.15-5.42) when compared with unexposed infants. The prevalence of wheezing among nonwhite infants was at least twice that of white infants, regardless of exposure. Infants living less than 400 m from a high volume of moving traffic, however, did not have an increased prevalence of wheezing. These results suggest that the distance from and type of traffic exposures are more significant risk factors than traffic volume for wheezing in early infancy.

  6. PENGEMBANGAN SISTEM PENGENDALIAN TRAFFIC DAN WEB FILTERING PADA JARINGAN INTERNET BERBASIS HOTSPOT

    Directory of Open Access Journals (Sweden)

    Riska Robianto

    2016-12-01

    Full Text Available Utilization of Internet services to the hotspot network raises particular problems in traffic control and web filtering. Linux as an operating system that is open (open source, offers a variety of internet firewall systems to assist in the management of traffic, bandwidth, web filtering and one using Smoothwall Express to Linux which can convert a personal computer (PC to the Internet firewall reliable and stable. Smoothwall allows to integrated with the add-ons extends like AdvProxy and URL Filter to improve the performance of Smoohtwall and simplify configuration of the traffic control and web filtering. The research was conducted by collecting data from various sources related, then experiment with implementing Smoohtwall by adding add-ons extends to help administrators manage and regulate the bandwidth allocation of each client, block websites (URLs that are not needed. Based on these results bandwidth management can be divided equally and can block websites (URLs that are not needed like porn sites, warez, bad word, and also the quality of the network connection updatesites more stable.

  7. Reconfigurable origami sonic barriers with tunable bandgaps for traffic noise mitigation

    Science.gov (United States)

    Thota, M.; Wang, K. W.

    2017-10-01

    An origami sonic barrier composed of cylindrical inclusions attached onto an origami sheet is proposed. The idea allows for tunable sound blocking properties for application in attenuating complex traffic noise spectra. Folding of the underlying origami sheet transforms the periodicity of the inclusions between different Bravais lattices, viz. between a square and a hexagonal lattice, and such significant lattice re-configuration leads to drastic tuning of dispersion characteristics. The wave tuning capabilities are corroborated via performing theoretical and numerical investigations using a plane wave expansion method and an acoustic simulation package of COMSOL, while experiments are performed on a one-seventh scaled-down model of origami sonic barrier to demonstrate the lattice re-configuration between different Bravais lattices and the associated bandgap adaptability. Good sound blocking performance in the frequency range of traffic noise spectra combined with less efforts, required for actuating one-degree of freedom folding mechanism, makes the origami sonic barrier a potential candidate for mitigating complex traffic noise.

  8. Development of Traffic Accidents Control System

    Directory of Open Access Journals (Sweden)

    Andrey Borisovich Nikolaev

    2015-05-01

    Full Text Available Proposed a structure of traffic accidents control system included three main parts: pre-processing, decision support and monitoring. For decision support systems we propose a method that allows to make decisions on the basis of fuzzy situational management. The advantage of the method: it allows to formalize a set of typical traffic situations, using the theory of fuzzy sets and to carry out selection of the desired management action.

  9. Network Characteristics of Video Streaming Traffic

    Science.gov (United States)

    2011-11-01

    Silverlight, Flash, or HTML5 ) used for video streaming. In particular, we identify three different streaming strategies that produce traffic... HTML5 , Flash. 1. INTRODUCTION The popularity of video streaming has considerably increased in the last decade. Indeed, recent studies have shown...applications for mobile devices), and the container (Flash [10], HTML5 [18], Silverlight [4]), on the charac- teristics of the traffic between the

  10. Human Factors in Air Traffic Control

    Science.gov (United States)

    1982-04-01

    knowlodg of the world from our experience, undverstanding, end acquisition of’ information. These divisions do not fit practical considerations nuatly...Clearly in air tra.ffic control the coan-troller relies liavily on long term memory for hei general knowlodge of the principles, methods and techniques of...air traffic control and for his specific knowledge about the way those prinoiples are translated into practice in a partioalar air space under his own

  11. Cost of Road Traffic Accidents in Egypt

    OpenAIRE

    Mohamed A. Ismail; Samar M. M. Abdelmageed

    2010-01-01

    The main objective of this paper is to estimate the cost of road traffic accidents in Egypt. The Human Capital (HC) approach, specifically the Gross-Loss-of-Output methodology, is adopted for estimation. Moreover, cost values obtained by previous national literature are updated using the inflation rates. The results indicate an estimated cost of road traffic accidents in Egypt of approximately 10 billion Egyptian Pounds (about $US 1.8 billion) for the year 2008. In addition, it is expected th...

  12. Road traffic noise and children's inattention.

    Science.gov (United States)

    Weyde, Kjell Vegard; Krog, Norun Hjertager; Oftedal, Bente; Magnus, Per; Øverland, Simon; Stansfeld, Stephen; Nieuwenhuijsen, Mark J; Vrijheid, Martine; de Castro Pascual, Montserrat; Aasvang, Gunn Marit

    2017-11-21

    An increasing number of children are exposed to road traffic noise levels that may lead to adverse effects on health and daily functioning. Childhood is a period of intense growth and brain maturation, and children may therefore be especially vulnerable to road traffic noise. The objective of the present study was to examine whether road traffic noise was associated with reported inattention symptoms in children, and whether this association was mediated by sleep duration. This study was based on the Norwegian Mother and Child Cohort Study conducted by the Norwegian Institute of Public Health. Parental reports of children's inattention at age 8 were linked to modelled levels of residential road traffic noise. We investigated the association between inattention and noise exposure during pregnancy (n = 1934), noise exposure averaged over 5 years (age 3 to 8 years; n = 1384) and noise exposure at age 8 years (n = 1384), using fractional logit response models. The participants were children from Oslo, Norway. An association with inattention at age 8 years was found for road traffic noise exposure at age 8 years (coef = .0083, CI = [.0012, .0154]; 1.2% point increase in inattention score per 10 dB increase in noise level), road traffic noise exposure average for the last 5 years (coef = .0090, CI = [.0016, .0164]; 1.3% point increase/10 dB), and for pregnancy road traffic noise exposure for boys (coef = .0091, CI = [.0010, .0171]), but not girls (coef = -.0021, CI = [-.0094, .0053]). Criteria for doing mediation analyses were not fulfilled. Results indicate that road traffic noise has a negative impact on children's inattention. We found no mediation by sleep duration.

  13. Do Traffic Controllers Dream of Electric Cars?

    OpenAIRE

    Taylor, N.

    2015-01-01

    Traffic congestion is not a modern phenomenon, but the growth of motor traffic driven by the supply of petroleum is. This coincides with concern about climate change caused by carbon emissions to which transport makes a substantial and increasing contribution. It seems that private cars in particular cannot be useful without coming to dominate human activity and even whole economies, creating dependence, congestion and severance which paradoxically negate many of the benefits of mobility. Ele...

  14. Models of Weather Impact on Air Traffic

    Science.gov (United States)

    Kulkarni, Deepak; Wang, Yao

    2017-01-01

    Flight delays have been a serious problem in the national airspace system costing about $30B per year. About 70 of the delays are attributed to weather and upto two thirds of these are avoidable. Better decision support tools would reduce these delays and improve air traffic management tools. Such tools would benefit from models of weather impacts on the airspace operations. This presentation discusses use of machine learning methods to mine various types of weather and traffic data to develop such models.

  15. Convergence of Sample Autocorrelation of Long-Range Dependent Traffic

    Directory of Open Access Journals (Sweden)

    Ming Li

    2013-01-01

    Full Text Available We depict our work on a fundamental issue in the theory of long-range dependent traffic in the aspect of the convergence of sample autocorrelation function (ACF of real traffic. The present results suggest that the sample ACF of traffic is convergent. In addition, we show that the sample size has considerable effects in estimating the sample ACF of traffic. More precisely, a sample ACF of traffic tends to be smoother when the sample size increases.

  16. Smoothing the Sample Autocorrelation of Long-Range-Dependent Traffic

    Directory of Open Access Journals (Sweden)

    Ming Li

    2013-01-01

    Full Text Available This paper depicts our work in smoothing the sample autocorrelation function (ACF of traffic. The experimental results exhibit that the sample ACF of traffic may be smoothed by the way of average. In addition, the results imply that the sum of sample ACFs of traffic convergences. Considering that the traffic data used in this research is long-range dependent (LRD, the latter may be meaningful for the theoretical research of LRD traffic.

  17. A Machine Learning Approach to Air Traffic Route Choice Modelling

    OpenAIRE

    Marcos, Rodrigo; García-Cantú, Oliva; Herranz, Ricardo

    2018-01-01

    Air Traffic Flow and Capacity Management (ATFCM) is one of the constituent parts of Air Traffic Management (ATM). The goal of ATFCM is to make airport and airspace capacity meet traffic demand and, when capacity opportunities are exhausted, optimise traffic flows to meet the available capacity. One of the key enablers of ATFCM is the accurate estimation of future traffic demand. The available information (schedules, flight plans, etc.) and its associated level of uncertainty differ across the...

  18. Evaluation of Deterministic and Stochastic Components of Traffic Counts

    Directory of Open Access Journals (Sweden)

    Ivan Bošnjak

    2012-10-01

    Full Text Available Traffic counts or statistical evidence of the traffic processare often a characteristic of time-series data. In this paper fundamentalproblem of estimating deterministic and stochasticcomponents of a traffic process are considered, in the context of"generalised traffic modelling". Different methods for identificationand/or elimination of the trend and seasonal componentsare applied for concrete traffic counts. Further investigationsand applications of ARIMA models, Hilbert space formulationsand state-space representations are suggested.

  19. System and method for traffic signal timing estimation

    KAUST Repository

    Dumazert, Julien

    2015-12-30

    A method and system for estimating traffic signals. The method and system can include constructing trajectories of probe vehicles from GPS data emitted by the probe vehicles, estimating traffic signal cycles, combining the estimates, and computing the traffic signal timing by maximizing a scoring function based on the estimates. Estimating traffic signal cycles can be based on transition times of the probe vehicles starting after a traffic signal turns green.

  20. DRONE - a tool for urban traffic noise abatement policy evaluation

    OpenAIRE

    Bhaskar, A.; Chung, E.; Kuwahara, M.

    2005-01-01

    Areawide Dynamic ROad traffic NoisE simulator-DRONE, been has developed by the integration of a road traffic noise prediction model (ASJ MODEL-1998) with road traffic simulator (AVENUE) which is further linked with GIS to provide areawide dynamic road traffic noise contour maps. This tool provides an engineering solution for the prediction of road traffic noise in urban network. Noise reduction in an urban area can be achieved by noise abatement policie...

  1. Modelling of H.264 MPEG2 TS Traffic Source

    Directory of Open Access Journals (Sweden)

    Stanislav Klucik

    2013-01-01

    Full Text Available This paper deals with IPTV traffic source modelling. Traffic sources are used for simulation, emulation and real network testing. This model is made as a derivation of known recorded traffic sources that are analysed and statistically processed. As the results show the proposed model causes in comparison to the known traffic source very similar network traffic parameters when used in a simulated network.

  2. Adaptive traffic control systems for urban networks

    Directory of Open Access Journals (Sweden)

    Radivojević Danilo

    2017-01-01

    Full Text Available Adaptive traffic control systems represent complex, but powerful tool for improvement of traffic flow conditions in locations or zones where applied. Many traffic agencies, especially those that have a large number of signalized intersections with high variability of the traffic demand, choose to apply some of the adaptive traffic control systems. However, those systems are manufactured and offered by multiple vendors (companies that are competing for the market share. Due to that fact, besides the information available from the vendors themselves, or the information from different studies conducted on different continents, very limited amount of information is available about the details how those systems are operating. The reason for that is the protecting of the intellectual property from plagiarism. The primary goal of this paper is to make a brief analysis of the functionalities, characteristics, abilities and results of the most recognized, but also less known adaptive traffic control systems to the professional public and other persons with interest in this subject.

  3. Optimizing Traffic Operation in Designing Specific Upgrades

    Directory of Open Access Journals (Sweden)

    Ebrahim Sangsefidi

    2015-01-01

    Full Text Available Transport forms one of the primary needs in all categories of the population in modern society; it is of paramount concern for traffic engineers, transport planners, and policy makers to understand and evaluate the quality of service being provided by the transport facilities designed by them. This paper presents an investigation in profile geometric design and traffic flow operation on two-lane two-way highways and provides analyses that will help in a better understanding of traffic operation on these facilities to select the optimum profile configuration. The effects of influencing parameters consisting of grade, length of grade, traffic composition, and traffic volume are evaluated and finally a systematic procedure to evaluate flow rate under the base condition is presented. Finally, based on these achievements an algorithm is introduced to select optimum Finished Ground of profile view. Results show that the percentage of heavy vehicles has a contributing effect on traffic operation so that the optimum profile configuration is incredibly affected by this factor. Source data have been obtained from Highway Capacity Manual (HCM as a pioneer document in respect of quantifying the concept of capacity for a transport facility.

  4. Congestion and communication in confined ant traffic

    Science.gov (United States)

    Gravish, Nick; Gold, Gregory; Zangwill, Andrew; Goodisman, Michael A. D.; Goldman, Daniel I.

    2014-03-01

    Many social animals move and communicate within confined spaces. In subterranean fire ants Solenopsis invicta, mobility within crowded nest tunnels is important for resource and information transport. Within confined tunnels, communication and traffic flow are at odds: trafficking ants communicate through tactile interactions while stopped, yet ants that stop to communicate impose physical obstacles on the traffic. We monitor the bi-directional flow of fire ant workers in laboratory tunnels of varied diameter D. The persistence time of communicating ant aggregations, τ, increases approximately linearly with the number of participating ants, n. The sensitivity of traffic flow increases as D decreases and diverges at a minimum diameter, Dc. A cellular automata model incorporating minimal traffic features--excluded volume and communication duration--reproduces features of the experiment. From the model we identify a competition between information transfer and the need to maintain jam-free traffic flow. We show that by balancing information transfer and traffic flow demands, an optimum group strategy exists which maximizes information throughput. We acknowledge funding from NSF PoLS #0957659 and #PHY-1205878.

  5. Competitive Traffic Assignment in Road Networks

    Directory of Open Access Journals (Sweden)

    Krylatov Alexander Y.

    2016-09-01

    Full Text Available Recently in-vehicle route guidance and information systems are rapidly developing. Such systems are expected to reduce congestion in an urban traffic area. This social benefit is believed to be reached by imposing the route choices on the network users that lead to the system optimum traffic assignment. However, guidance service could be offered by different competitive business companies. Then route choices of different mutually independent groups of users may reject traffic assignment from the system optimum state. In this paper, a game theoretic approach is shown to be very efficient to formalize competitive traffic assignment problem with various groups of users in the form of non-cooperative network game with the Nash equilibrium search. The relationships between the Wardrop’s system optimum associated with the traffic assignment problem and the Nash equilibrium associated with the competitive traffic assignment problem are investigated. Moreover, some related aspects of the Nash equilibrium and the Wardrop’s user equilibrium assignments are also discussed.

  6. Traffic flow wide-area surveillance system

    Science.gov (United States)

    Allgood, Glenn O.; Ferrell, Regina K.; Kercel, Stephen W.; Abston, Ruth A.

    1995-01-01

    Traffic management can be thought of as a stochastic queuing process where the serving time at one of its control points is dynamically linked to the global traffic pattern, which is, in turn, dynamically linked to the control point. For this closed-loop system to be effective, the traffic management system must sense and interpret a large spatial projection of data originating from multiple sensor suites. This concept is the basis for the development of a traffic flow wide-area surveillance (TFWAS) system. This paper presents the results of a study by Oak Ridge National Laboratory to define the operational specifications and characteristics, to determine the constraints, and to examine the state of technology of a TFWAS system in terms of traffic management and control. In doing so, the functions and attributes of a TFWAS system are mapped into an operational structure consistent with the Intelligent Vehicle Highway System (IVHS) concept and the existing highway infrastructure. This mapping includes identifying candidate sensor suites and establishing criteria, requirements, and performance measures by which these systems can be graded in their ability and practicality to meet the operational requirements of a TFWAS system. In light of this, issues such as system integration, applicable technologies, impact on traffic management and control, and public acceptance are addressed.

  7. Left bundle-branch block

    DEFF Research Database (Denmark)

    Risum, Niels; Strauss, David; Sogaard, Peter

    2013-01-01

    The relationship between myocardial electrical activation by electrocardiogram (ECG) and mechanical contraction by echocardiography in left bundle-branch block (LBBB) has never been clearly demonstrated. New strict criteria for LBBB based on a fundamental understanding of physiology have recently...

  8. The wild tapered block bootstrap

    DEFF Research Database (Denmark)

    Hounyo, Ulrich

    -based method in terms of asymptotic accuracy of variance estimation and distribution approximation. For stationary time series, the asymptotic validity, and the favorable bias properties of the new bootstrap method are shown in two important cases: smooth functions of means, and M-estimators. The first......-order asymptotic validity of the tapered block bootstrap as well as the wild tapered block bootstrap approximation to the actual distribution of the sample mean is also established when data are assumed to satisfy a near epoch dependent condition. The consistency of the bootstrap variance estimator for the sample......In this paper, a new resampling procedure, called the wild tapered block bootstrap, is introduced as a means of calculating standard errors of estimators and constructing confidence regions for parameters based on dependent heterogeneous data. The method consists in tapering each overlapping block...

  9. Recursion Relations for Conformal Blocks

    CERN Document Server

    Penedones, João; Yamazaki, Masahito

    2016-09-12

    In the context of conformal field theories in general space-time dimension, we find all the possible singularities of the conformal blocks as functions of the scaling dimension $\\Delta$ of the exchanged operator. In particular, we argue, using representation theory of parabolic Verma modules, that in odd spacetime dimension the singularities are only simple poles. We discuss how to use this information to write recursion relations that determine the conformal blocks. We first recover the recursion relation introduced in 1307.6856 for conformal blocks of external scalar operators. We then generalize this recursion relation for the conformal blocks associated to the four point function of three scalar and one vector operator. Finally we specialize to the case in which the vector operator is a conserved current.

  10. Defying gravity using Jenga™ blocks

    Science.gov (United States)

    Tan, Yin-Soo; Yap, Kueh-Chin

    2007-11-01

    This paper describes how Jenga™ blocks can be used to demonstrate the physics of an overhanging tower that appears to defy gravity. We also propose ideas for how this demonstration can be adapted for the A-level physics curriculum.

  11. Impacts of Traffic Noise and Traffic Volume on Birds of Roadside Habitats

    Directory of Open Access Journals (Sweden)

    Kirsten M. Parris

    2009-06-01

    Full Text Available Roadside habitats are important for a range of taxa including plants, insects, mammals, and birds, particularly in developed countries in which large expanses of native vegetation have been cleared for agriculture or urban development. Although roadside vegetation may provide suitable habitat for many species, resident animals can be exposed to high levels of traffic noise, visual disturbance from passing vehicles, and the risk of collision with cars and trucks. Traffic noise can reduce the distance over which acoustic signals such as song can be detected, an effect known as acoustic interference or masking. Studies from the northern hemisphere show that the singing behavior of birds changes in the presence of traffic noise. We investigated the impact of traffic noise and traffic volume on two species of birds, the Grey Shrike-thrush (Colluricincla harmonica and the Grey Fantail (Rhipidura fuliginosa, at 58 roadside sites on the Mornington Peninsula, southeastern Australia. The lower singing Grey Shrike-thrush sang at a higher frequency in the presence of traffic noise, with a predicted increase in dominant frequency of 5.8 Hz/dB of traffic noise, and a total effect size of 209 Hz. In contrast, the higher singing Grey Fantail did not appear to change its song in traffic noise. The probability of detecting each species on a visit to a site declined substantially with increasing traffic noise and traffic volume, with several lines of evidence supporting a larger effect of traffic noise. Traffic noise could hamper detection of song by conspecifics, making it more difficult for birds to establish and maintain territories, attract mates and maintain pair bonds, and possibly leading to reduced breeding success in noisy roadside habitats. Closing key roads during the breeding season is a potential, but untested, management strategy to protect threatened bird species from traffic noise and collision with vehicles at the time of year when they are most

  12. Real-time sensing of enteropathogenic E. coli-induced effects on epithelial host cell height, cell-substrate interactions, and endocytic processes by infrared surface plasmon spectroscopy.

    Directory of Open Access Journals (Sweden)

    Victor Yashunsky

    Full Text Available Enteropathogenic Escherichia coli (EPEC is an important, generally non-invasive, bacterial pathogen that causes diarrhea in humans. The microbe infects mainly the enterocytes of the small intestine. Here we have applied our newly developed infrared surface plasmon resonance (IR-SPR spectroscopy approach to study how EPEC infection affects epithelial host cells. The IR-SPR experiments showed that EPEC infection results in a robust reduction in the refractive index of the infected cells. Assisted by confocal and total internal reflection microscopy, we discovered that the microbe dilates the intercellular gaps and induces the appearance of fluid-phase-filled pinocytic vesicles in the lower basolateral regions of the host epithelial cells. Partial cell detachment from the underlying substratum was also observed. Finally, the waveguide mode observed by our IR-SPR analyses showed that EPEC infection decreases the host cell's height to some extent. Together, these observations reveal novel impacts of the pathogen on the host cell architecture and endocytic functions. We suggest that these changes may induce the infiltration of a watery environment into the host cell, and potentially lead to failure of the epithelium barrier functions. Our findings also indicate the great potential of the label-free IR-SPR approach to study the dynamics of host-pathogen interactions with high spatiotemporal sensitivity.

  13. Ubiquitination of human leukocyte antigen (HLA)-DM by different membrane-associated RING-CH (MARCH) protein family E3 ligases targets different endocytic pathways.

    Science.gov (United States)

    Jahnke, Martin; Trowsdale, John; Kelly, Adrian P

    2012-03-02

    HLA-DM plays an essential role in the peptide loading of classical class II molecules and is present both at the cell surface and in late endosomal peptide-loading compartments. Trafficking of DM within antigen-presenting cells is complex and is, in part, controlled by a tyrosine-based targeting signal present in the cytoplasmic tail of DMβ. Here, we show that DM also undergoes post-translational modification through ubiquitination of a single lysine residue present in the cytoplasmic tail of the α chain, DMα. Ubiquitination of DM by MARCH1 and MARCH9 induced loss of DM molecules from the cell surface by a mechanism that cumulatively involved both direct attachment of ubiquitin chains to DMα and a functional tyrosine-based signal on DMβ. In contrast, MARCH8-induced loss of surface DM was entirely dependent upon the tyrosine signal on DMβ. In the absence of this tyrosine residue, levels of DM remained unchanged irrespective of whether DMα was ubiquitinated by MARCH8. The influence of MARCH8 was indirect and may have resulted from modification of components of the endocytic machinery by ubiquitination.

  14. Ubiquitination of Human Leukocyte Antigen (HLA)-DM by Different Membrane-associated RING-CH (MARCH) Protein Family E3 Ligases Targets Different Endocytic Pathways*

    Science.gov (United States)

    Jahnke, Martin; Trowsdale, John; Kelly, Adrian P.

    2012-01-01

    HLA-DM plays an essential role in the peptide loading of classical class II molecules and is present both at the cell surface and in late endosomal peptide-loading compartments. Trafficking of DM within antigen-presenting cells is complex and is, in part, controlled by a tyrosine-based targeting signal present in the cytoplasmic tail of DMβ. Here, we show that DM also undergoes post-translational modification through ubiquitination of a single lysine residue present in the cytoplasmic tail of the α chain, DMα. Ubiquitination of DM by MARCH1 and MARCH9 induced loss of DM molecules from the cell surface by a mechanism that cumulatively involved both direct attachment of ubiquitin chains to DMα and a functional tyrosine-based signal on DMβ. In contrast, MARCH8-induced loss of surface DM was entirely dependent upon the tyrosine signal on DMβ. In the absence of this tyrosine residue, levels of DM remained unchanged irrespective of whether DMα was ubiquitinated by MARCH8. The influence of MARCH8 was indirect and may have resulted from modification of components of the endocytic machinery by ubiquitination. PMID:22247549

  15. Protocol vulnerability detection based on network traffic analysis and binary reverse engineering.

    Science.gov (United States)

    Wen, Shameng; Meng, Qingkun; Feng, Chao; Tang, Chaojing

    2017-01-01

    Network protocol vulnerability detection plays an important role in many domains, including protocol security analysis, application security, and network intrusion detection. In this study, by analyzing the general fuzzing method of network protocols, we propose a novel approach that combines network traffic analysis with the binary reverse engineering method. For network traffic analysis, the block-based protocol description language is introduced to construct test scripts, while the binary reverse engineering method employs the genetic algorithm with a fitness function designed to focus on code coverage. This combination leads to a substantial improvement in fuzz testing for network protocols. We build a prototype system and use it to test several real-world network protocol implementations. The experimental results show that the proposed approach detects vulnerabilities more efficiently and effectively than general fuzzing methods such as SPIKE.

  16. Protocol vulnerability detection based on network traffic analysis and binary reverse engineering.

    Directory of Open Access Journals (Sweden)

    Shameng Wen

    Full Text Available Network protocol vulnerability detection plays an important role in many domains, including protocol security analysis, application security, and network intrusion detection. In this study, by analyzing the general fuzzing method of network protocols, we propose a novel approach that combines network traffic analysis with the binary reverse engineering method. For network traffic analysis, the block-based protocol description language is introduced to construct test scripts, while the binary reverse engineering method employs the genetic algorithm with a fitness function designed to focus on code coverage. This combination leads to a substantial improvement in fuzz testing for network protocols. We build a prototype system and use it to test several real-world network protocol implementations. The experimental results show that the proposed approach detects vulnerabilities more efficiently and effectively than general fuzzing methods such as SPIKE.

  17. Consensus-Based Cooperative Control Based on Pollution Sensing and Traffic Information for Urban Traffic Networks

    Directory of Open Access Journals (Sweden)

    Antonio Artuñedo

    2017-04-01

    Full Text Available Nowadays many studies are being conducted to develop solutions for improving the performance of urban traffic networks. One of the main challenges is the necessary cooperation among different entities such as vehicles or infrastructure systems and how to exploit the information available through networks of sensors deployed as infrastructures for smart cities. In this work an algorithm for cooperative control of urban subsystems is proposed to provide a solution for mobility problems in cities. The interconnected traffic lights controller (TLC network adapts traffic lights cycles, based on traffic and air pollution sensory information, in order to improve the performance of urban traffic networks. The presence of air pollution in cities is not only caused by road traffic but there are other pollution sources that contribute to increase or decrease the pollution level. Due to the distributed and heterogeneous nature of the different components involved, a system of systems engineering approach is applied to design a consensus-based control algorithm. The designed control strategy contains a consensus-based component that uses the information shared in the network for reaching a consensus in the state of TLC network components. Discrete event systems specification is applied for modelling and simulation. The proposed solution is assessed by simulation studies with very promising results to deal with simultaneous responses to both pollution levels and traffic flows in urban traffic networks.

  18. Consensus-Based Cooperative Control Based on Pollution Sensing and Traffic Information for Urban Traffic Networks.

    Science.gov (United States)

    Artuñedo, Antonio; Del Toro, Raúl M; Haber, Rodolfo E

    2017-04-26

    Nowadays many studies are being conducted to develop solutions for improving the performance of urban traffic networks. One of the main challenges is the necessary cooperation among different entities such as vehicles or infrastructure systems and how to exploit the information available through networks of sensors deployed as infrastructures for smart cities. In this work an algorithm for cooperative control of urban subsystems is proposed to provide a solution for mobility problems in cities. The interconnected traffic lights controller ( TLC ) network adapts traffic lights cycles, based on traffic and air pollution sensory information, in order to improve the performance of urban traffic networks. The presence of air pollution in cities is not only caused by road traffic but there are other pollution sources that contribute to increase or decrease the pollution level. Due to the distributed and heterogeneous nature of the different components involved, a system of systems engineering approach is applied to design a consensus-based control algorithm. The designed control strategy contains a consensus-based component that uses the information shared in the network for reaching a consensus in the state of TLC network components. Discrete event systems specification is applied for modelling and simulation. The proposed solution is assessed by simulation studies with very promising results to deal with simultaneous responses to both pollution levels and traffic flows in urban traffic networks.

  19. Monitoring road traffic congestion using a macroscopic traffic model and a statistical monitoring scheme

    KAUST Repository

    Zeroual, Abdelhafid

    2017-08-19

    Monitoring vehicle traffic flow plays a central role in enhancing traffic management, transportation safety and cost savings. In this paper, we propose an innovative approach for detection of traffic congestion. Specifically, we combine the flexibility and simplicity of a piecewise switched linear (PWSL) macroscopic traffic model and the greater capacity of the exponentially-weighted moving average (EWMA) monitoring chart. Macroscopic models, which have few, easily calibrated parameters, are employed to describe a free traffic flow at the macroscopic level. Then, we apply the EWMA monitoring chart to the uncorrelated residuals obtained from the constructed PWSL model to detect congested situations. In this strategy, wavelet-based multiscale filtering of data has been used before the application of the EWMA scheme to improve further the robustness of this method to measurement noise and reduce the false alarms due to modeling errors. The performance of the PWSL-EWMA approach is successfully tested on traffic data from the three lane highway portion of the Interstate 210 (I-210) highway of the west of California and the four lane highway portion of the State Route 60 (SR60) highway from the east of California, provided by the Caltrans Performance Measurement System (PeMS). Results show the ability of the PWSL-EWMA approach to monitor vehicle traffic, confirming the promising application of this statistical tool to the supervision of traffic flow congestion.

  20. Traffic planning and air pollution - The Bordeaux example. I - The GERTRUDE traffic plan

    Energy Technology Data Exchange (ETDEWEB)

    Franceries, Mr.

    1980-01-01

    The GERTRUDE global traffic plan intended for the simultaneous improvement of resident mobility and air quality in the city of Bordeaux is presented. The plan is designed to meet road user requirements of safety, public transportation efficiency and improved general traffic flow and passenger comfort, by making use of a master traffic plan, a good representation of traffic patterns in the center of the city, including bottleneck areas, and an automatic traffic light control system, operating on the basis of traffic flow measurements at strategic intersections. Provisions are also included for the expedited passage of emergency vehicles and public transportation. Operation of the system has led to nearly 50 percent increase in mean traffic flow rates through the major arteries of the city center, with considerable decreases in mean transit time and accident rates and a significant improvement in automobile pollution levels. The Bordeaux traffic system has also been applied to the direct reduction of automobile pollution using CO and noise probes, and the utilization of meteorological data as additional system inputs is planned.

  1. Environmental pollution of lead in traffic air and blood of traffic ...

    African Journals Online (AJOL)

    The blood samples of all policemen were analyzed by atomic absorption Spectrophotometer to determine lead levels. A questionnaire was designed to assess the adverse health effects on the traffic policemen. Results: The degree of environmental lead pollution in traffic ambient air was found to be 0.1937}0.1768 mg/m3 ...

  2. Criminal Liability for Serious Traffic Offences : Essays on Causing Death, Injury and Danger in Traffic

    NARCIS (Netherlands)

    van Dijk, Alwin; Wolswijk, Hein

    2015-01-01

    The criminal law on serious traffic offences is a fascinating area. The traffic context presents legislators with numerous controversial issues. One such issue is when severe consequences are matched with low moral culpability. How should the law deal with a driver who kills someone because she

  3. Analysis of Separated Flow over Blocked Surface

    Directory of Open Access Journals (Sweden)

    Onur YEMENİCİ

    2013-04-01

    Full Text Available In this study, the separated flow over flat and blocked surfaces was investigated experimentally. Velocity and turbulence intensity measurements were carried out by a constanttemperature hot wire anemometer and static pressure measurements by a micro-manometer. The flow separations and reattachments were occurred before the first block, on the first block, between blocks and after the last block, and the presence of the blocks significantly increased the turbulent intensity

  4. The impact of traffic sign deficit on road traffic accidents in Nigeria.

    Science.gov (United States)

    Ezeibe, Christian; Ilo, Chukwudi; Oguonu, Chika; Ali, Alphonsus; Abada, Ifeanyi; Ezeibe, Ezinwanne; Oguonu, Chukwunonso; Abada, Felicia; Izueke, Edwin; Agbo, Humphrey

    2018-04-04

    This study assesses the impact of traffic sign deficit on road traffic accidents in Nigeria. The participants were 720 commercial vehicle drivers. While simple random sampling was used to select 6 out of 137 federal highways, stratified random sampling was used to select six categories of commercial vehicle drivers. The study used qual-dominant mixed methods approach comprising key informant interviews; group interviews; field observation; policy appraisal and secondary literature on traffic signs. Result shows that the failure of government to provide and maintain traffic signs in order to guide road users through the numerous accident black spots on the highways is the major cause of road accidents in Nigeria. The study argues that provision and maintenance of traffic signs present opportunity to promoting safety on the highways and achieving the sustainable development goals.

  5. A neural network methodology for traffic congestion prediction and online traffic control

    Energy Technology Data Exchange (ETDEWEB)

    Demmou, H.; Hermitte, L.; Sahraoui, A. [Centre National de la Recherche Scientifique (CNRS), 31 - Toulouse (France). Laboratoire d`Analyse et d`Architecture des Systemes; Olivero, P. [ZELT, 31 - Toulouse (France)

    1998-12-01

    The aim of this paper is to present an approach for traffic light signals control and traffic congestion prediction based on the neural network technique. We propose to define and implement a modular and integrated functional structure to realize these two very important tasks when facing traffic problems. A methodology for Neural Network approach is proposed. The approach is intended for transportation systems and mainly for urban traffic control. The main objectives of the work are two fold; the first is to develop a generic methodology for Neural Network approach; the second is to enhance such development for transportation issues mainly in urban traffic congestion detection, congestion prediction, supervision and control. (authors) 5 refs.

  6. Adaptive traffic signal control with actor-critic methods in a real-world traffic network with different traffic disruption events

    NARCIS (Netherlands)

    Aslani, Mohammad; Mesgari, Mohammad Saadi; Wiering, Marco

    2017-01-01

    The transportation demand is rapidly growing in metropolises, resulting in chronic traffic con-gestions in dense downtown areas. Adaptive traffic signal control as the principle part of in-telligent transportation systems has a primary role to effectively reduce traffic congestion by making a

  7. Spectral Analysis of Traffic Functions in Urban Areas

    Directory of Open Access Journals (Sweden)

    Florin Nemtanu

    2015-12-01

    Full Text Available The paper is focused on the Fourier transform application in urban traffic analysis and the use of said transform in traffic decomposition. The traffic function is defined as traffic flow generated by different categories of traffic participants. A Fourier analysis was elaborated in terms of identifying the main traffic function components, called traffic sub-functions. This paper presents the results of the method being applied in a real case situation, that is, an intersection in the city of Bucharest where the effect of a bus line was analysed. The analysis was done using different time scales, while three different traffic functions were defined to demonstrate the theoretical effect of the proposed method of analysis. An extension of the method is proposed to be applied in urban areas, especially in the areas covered by predictive traffic control.

  8. TRAFFIC SIGN DETECTION BASED ON BIOLOGICALLY VISUAL MECHANISM

    Directory of Open Access Journals (Sweden)

    X. Hu

    2012-07-01

    Full Text Available TSR (Traffic sign recognition is an important problem in ITS (intelligent traffic system, which is being paid more and more attention for realizing drivers assisting system and unmanned vehicle etc. TSR consists of two steps: detection and recognition, and this paper describe a new traffic sign detection method. The design principle of the traffic sign is comply with the visual attention mechanism of human, so we propose a method using visual attention mechanism to detect traffic sign ,which is reasonable. In our method, the whole scene will firstly be analyzed by visual attention model to acquire the area where traffic signs might be placed. And then, these candidate areas will be analyzed according to the shape characteristics of the traffic sign to detect traffic signs. In traffic sign detection experiments, the result shows the proposed method is effectively and robust than other existing saliency detection method.

  9. Bound Maxima as a Traffic Feature under DDOS Flood Attacks

    Directory of Open Access Journals (Sweden)

    Jie Xue

    2012-01-01

    Full Text Available This paper gives a novel traffic feature for identifying abnormal variation of traffic under DDOS flood attacks. It is the histogram of the maxima of the bounded traffic rate on an interval-by-interval basis. We use it to experiment on the traffic data provided by MIT Lincoln Laboratory under Defense Advanced Research Projects Agency (DARPA in 1999. The experimental results profitably enhance the evidences that traffic rate under DDOS attacks is statistically higher than that of normal traffic considerably. They show that the pattern of the histogram of the maxima of bounded rate of attack-contained traffic greatly differs from that of attack-free traffic. Besides, the present traffic feature is simple in mathematics and easy to use in practice.

  10. Various semiclassical limits of torus conformal blocks

    Energy Technology Data Exchange (ETDEWEB)

    Alkalaev, Konstantin [I.E. Tamm Department of Theoretical Physics, P.N. Lebedev Physical Institute,Leninsky ave. 53, Moscow, 119991 (Russian Federation); Department of General and Applied Physics, Moscow Institute of Physics and Technology,Institutskiy per. 7, Dolgoprudnyi, Moscow region, 141700 (Russian Federation); Geiko, Roman [Mathematics Department, National Research University Higher School of Economics,Usacheva str. 6, Moscow, 119048 (Russian Federation); Rappoport, Vladimir [I.E. Tamm Department of Theoretical Physics, P.N. Lebedev Physical Institute,Leninsky ave. 53, Moscow, 119991 (Russian Federation); Department of Quantum Physics, Institute for Information Transmission Problems,Bolshoy Karetny per. 19, Moscow, 127994 (Russian Federation)

    2017-04-12

    We study four types of one-point torus blocks arising in the large central charge regime. There are the global block, the light block, the heavy-light block, and the linearized classical block, according to different regimes of conformal dimensions. It is shown that the blocks are not independent being connected to each other by various links. We find that the global, light, and heavy-light blocks correspond to three different contractions of the Virasoro algebra. Also, we formulate the c-recursive representation of the one-point torus blocks which is relevant in the semiclassical approximation.

  11. Increase the Safety of Road Traffic Accidents by Applying Clustering

    Directory of Open Access Journals (Sweden)

    Kos Goran

    2013-12-01

    Full Text Available In terms of continual increase of number of traffic accidents and alarming trend of increasing number of traffic accidents with catastrophic consequences for human life and health, it is necessary to actively research and develop methods to combat these trends. One of the measures is the implementation of advanced information systems in existing traffic environment. Accidents clusters, as databases of traffic accidents, introduce a new dimension in traffic systems in the form of experience, providing information on current accidents and the ones that have previously occurred in a given period. This paper proposes a new approach to predictive management of traffic processes, based on the collection of data in real time and is based on accidents clusters. The modern traffic information services collects road traffic status data from a wide variety of traffic sensing systems using modern ICT technologies, creating the most accurate road traffic situation awareness achieved so far. Road traffic situation awareness enhanced by accident clusters' data can be visualized and distributed in various ways (including the forms of dynamic heat maps and on various information platforms, suiting the requirements of the end-users. Accent is placed on their significant features that are based on additional knowledge about existing traffic processes and distribution of important traffic information in order to prevent and reduce traffic accidents.

  12. Occupant traffic estimation through structural vibration sensing

    Science.gov (United States)

    Pan, Shijia; Mirshekari, Mostafa; Zhang, Pei; Noh, Hae Young

    2016-04-01

    The number of people passing through different indoor areas is useful in various smart structure applications, including occupancy-based building energy/space management, marketing research, security, etc. Existing approaches to estimate occupant traffic include vision-, sound-, and radio-based (mobile) sensing methods, which have placement limitations (e.g., requirement of line-of-sight, quiet environment, carrying a device all the time). Such limitations make these direct sensing approaches difficult to deploy and maintain. An indirect approach using geophones to measure floor vibration induced by footsteps can be utilized. However, the main challenge lies in distinguishing multiple simultaneous walkers by developing features that can effectively represent the number of mixed signals and characterize the selected features under different traffic conditions. This paper presents a method to monitor multiple persons. Once the vibration signals are obtained, features are extracted to describe the overlapping vibration signals induced by multiple footsteps, which are used for occupancy traffic estimation. In particular, we focus on analysis of the efficiency and limitations of the four selected key features when used for estimating various traffic conditions. We characterize these features with signals collected from controlled impulse load tests as well as from multiple people walking through a real-world sensing area. In our experiments, the system achieves the mean estimation error of +/-0.2 people for different occupant traffic conditions (from one to four) using k-nearest neighbor classifier.

  13. Distraction-related road traffic collisions.

    Science.gov (United States)

    Eid, Hani O; Abu-Zidan, Fikri M

    2017-06-01

    We aimed to prospectively study distraction-related road traffic collision injuries, their contributory factors, severity, and outcome. Data were prospectively collected on all hospitalized road traffic collision trauma patients in Al-Ain City who were drivers at the collision time over one and half years. Driver's inattentive behaviors preceding the collision were collected by interviewing the admitted drivers. There were 444 drivers, 330 of them were fully oriented patients, out of them only 44 (13%) were distracted. Nineteen (5.8%) drivers were distracted by using mobile phones, 12 (3.6%) were pre-occupied with deep thinking, six (1.8%) were talking with other passengers, four (1.2%) were picking things in the vehicle, and three (0.9%) were using entertainment systems. The maximum distraction occurred during the time of 6 am - 12 noon when the traffic was crowded. There were no significant differences between distracted and non-distracted drivers in demographical and physiological factors, injured regions, and outcomes. Distraction of alert drivers causes 13% of road traffic collisions in Al-Ain city. About 40 percent of the distracted drivers involved in road traffic collisions (RTC) were using mobile phones. Our study supports the ban of use of cell phones while driving.

  14. Spatial resolution requirements for traffic-related air pollutant exposure evaluations

    Science.gov (United States)

    Batterman, Stuart; Chambliss, Sarah; Isakov, Vlad

    2014-09-01

    Vehicle emissions represent one of the most important air pollution sources in most urban areas, and elevated concentrations of pollutants found near major roads have been associated with many adverse health impacts. To understand these impacts, exposure estimates should reflect the spatial and temporal patterns observed for traffic-related air pollutants. This paper evaluates the spatial resolution and zonal systems required to estimate accurately intraurban and near-road exposures of traffic-related air pollutants. The analyses use the detailed information assembled for a large (800 km2) area centered on Detroit, Michigan, USA. Concentrations of nitrogen oxides (NOx) due to vehicle emissions were estimated using hourly traffic volumes and speeds on 9700 links representing all but minor roads in the city, the MOVES2010 emission model, the RLINE dispersion model, local meteorological data, a temporal resolution of 1 h, and spatial resolution as low as 10 m. Model estimates were joined with the corresponding shape files to estimate residential exposures for 700,000 individuals at property parcel, census block, census tract, and ZIP code levels. We evaluate joining methods, the spatial resolution needed to meet specific error criteria, and the extent of exposure misclassification. To portray traffic-related air pollutant exposure, raster or inverse distance-weighted interpolations are superior to nearest neighbor approaches, and interpolations between receptors and points of interest should not exceed about 40 m near major roads, and 100 m at larger distances. For census tracts and ZIP codes, average exposures are overestimated since few individuals live very near major roads, the range of concentrations is compressed, most exposures are misclassified, and high concentrations near roads are entirely omitted. While smaller zones improve performance considerably, even block-level data can misclassify many individuals. To estimate exposures and impacts of traffic

  15. Radial Coordinates for Conformal Blocks

    CERN Document Server

    Hogervorst, Matthijs

    2013-01-01

    We develop the theory of conformal blocks in CFT_d expressing them as power series with Gegenbauer polynomial coefficients. Such series have a clear physical meaning when the conformal block is analyzed in radial quantization: individual terms describe contributions of descendants of a given spin. Convergence of these series can be optimized by a judicious choice of the radial quantization origin. We argue that the best choice is to insert the operators symmetrically. We analyze in detail the resulting "rho-series" and show that it converges much more rapidly than for the commonly used variable z. We discuss how these conformal block representations can be used in the conformal bootstrap. In particular, we use them to derive analytically some bootstrap bounds whose existence was previously found numerically.

  16. Diversity Gain through Antenna Blocking

    Directory of Open Access Journals (Sweden)

    V. Dehghanian

    2012-01-01

    Full Text Available As part of the typical usage mode, interaction between a handheld receiver antenna and the operator's RF absorbing body and nearby objects is known to generate variability in antenna radiation characteristics through blocking and pattern changes. It is counterintuitive that random variations in blocking can result in diversity gain of practical applicability. This diversity gain is quantified from a theoretical and experimental perspective. Measurements carried out at 1947.5 MHz verify the theoretical predictions, and a diversity gain of 3.1 dB was measured through antenna blocking and based on the utilized measurement setup. The diversity gain can be exploited to enhance signal detectability of handheld receivers based on a single antenna in indoor multipath environments.

  17. Cryptanalysis of Selected Block Ciphers

    DEFF Research Database (Denmark)

    Alkhzaimi, Hoda A.

    , pseudorandom number generators, and authenticated encryption designs. For this reason a multitude of initiatives over the years has been established to provide a secure and sound designs for block ciphers as in the calls for Data Encryption Standard (DES) and Advanced Encryption Standard (AES), lightweight...... ciphers initiatives, and the Competition for Authenticated Encryption: Security, Applicability, and Robustness (CAESAR). In this thesis, we first present cryptanalytic results on different ciphers. We propose attack named the Invariant Subspace Attack. It is utilized to break the full block cipher...... on the family of lightweight block cipher SIMON that was published by the U.S National Security Agency (NSA). The ciphers are developed with optimization towards both hardware and software in mind. While the specification paper discusses design requirements and performance of the presented lightweight ciphers...

  18. Climatological features of blocking anticyclones

    International Nuclear Information System (INIS)

    Lupo, A.R.; Smith, P.J.; Oglesby, R.J.

    1994-01-01

    Several climatological studies have been previously performed using large observational data sets (i.e., 10 years or longer) in order to determine the predominant characteristics of blocking anticyclones, including favored development regions, duration, preferred seasonal occurrence, and frequency of occurrence. These studies have shown that blocking anticyclones occur most frequently from October to April over the eastern Atlantic and Pacific oceans downstream from both the North American and Asian continental regions and the storm track regions to the east of these continents. Some studies have also revealed the presence of a third region block formation in western Russia near 40 degrees E which is associated with another storm track region over the Mediterranean and western Asia

  19. Projectors, shadows, and conformal blocks

    Energy Technology Data Exchange (ETDEWEB)

    Simmons-Duffin, David [Jefferson Physical Laboratory, Harvard University,Cambridge, MA 02138 (United States)

    2014-04-24

    We introduce a method for computing conformal blocks of operators in arbitrary Lorentz representations in any spacetime dimension, making it possible to apply bootstrap techniques to operators with spin. The key idea is to implement the “shadow formalism' of Ferrara, Gatto, Grillo, and Parisi in a setting where conformal invariance is manifest. Conformal blocks in d-dimensions can be expressed as integrals over the projective null-cone in the “embedding space' ℝ{sup d+1,1}. Taking care with their analytic structure, these integrals can be evaluated in great generality, reducing the computation of conformal blocks to a bookkeeping exercise. To facilitate calculations in four-dimensional CFTs, we introduce techniques for writing down conformally-invariant correlators using auxiliary twistor variables, and demonstrate their use in some simple examples.

  20. Projectors, Shadows, and Conformal Blocks

    OpenAIRE

    Simmons-Duffin, David

    2012-01-01

    We introduce a method for computing conformal blocks of operators in arbitrary Lorentz representations in any spacetime dimension, making it possible to apply bootstrap techniques to operators with spin. The key idea is to implement the “shadow formalism” of Ferrara, Gatto, Grillo, and Parisi in a setting where conformal invariance is manifest. Conformal blocks in d -dimensions can be expressed as integrals over the projective null-cone in the “embedding space” $ \\mathbb{R} $ d +1,1 . Taking ...