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Sample records for blocks endocytic traffic

  1. Interferon-induced Sus scrofa Mx1 blocks endocytic traffic of incoming influenza A virus particles.

    Science.gov (United States)

    Palm, Mélanie; Garigliany, Mutien-Marie; Cornet, François; Desmecht, Daniel

    2010-01-01

    The interferon-induced Mx proteins of vertebrates are dynamin-like GTPases, some isoforms of which can additionally inhibit the life cycle of certain RNA viruses. Here we show that the porcine Mx1 protein (poMx1) inhibits replication of influenza A virus and we attempt to identify the step at which the viral life cycle is blocked. In infected cells expressing poMx1, the level of transcripts encoding the viral nucleoprotein is significantly lower than normal, even when secondary transcription is prevented by exposure to cycloheximide. This reveals that a pretranscriptional block participates to the anti-influenza activity. Binding and internalization of incoming virus particles are normal in the presence of poMx1 but centripetal traffic to the late endosomes is interrupted. Surprisingly but decisively, poMx1 significantly alters binding of early endosome autoantigen 1 to early endosomes and/or early endosome size and spatial distribution. This is compatible with impairment of traffic of the endocytic vesicles to the late endosomes.

  2. Endocytic traffic: vesicle fusion cascade in the early endosomes.

    Science.gov (United States)

    Brenner, Michael P

    2012-08-01

    New research shows that vesicles in the early endosomal network coalesce according to a classical theoretical description of aggregation put forward by Smoluchowski more than 100 years ago. This gives a new tool for unraveling complexities of the endocytic pathways.

  3. A-RAF kinase functions in ARF6 regulated endocytic membrane traffic.

    Directory of Open Access Journals (Sweden)

    Elena Nekhoroshkova

    Full Text Available BACKGROUND: RAF kinases direct ERK MAPK signaling to distinct subcellular compartments in response to growth factor stimulation. METHODOLOGY/PRINCIPAL FINDINGS: Of the three mammalian isoforms A-RAF is special in that one of its two lipid binding domains mediates a unique pattern of membrane localization. Specific membrane binding is retained by an N-terminal fragment (AR149 that corresponds to a naturally occurring splice variant termed DA-RAF2. AR149 colocalizes with ARF6 on tubular endosomes and has a dominant negative effect on endocytic trafficking. Moreover actin polymerization of yeast and mammalian cells is abolished. AR149/DA-RAF2 does not affect the internalization step of endocytosis, but trafficking to the recycling compartment. CONCLUSIONS/SIGNIFICANCE: A-RAF induced ERK activation is required for this step by activating ARF6, as A-RAF depletion or inhibition of the A-RAF controlled MEK-ERK cascade blocks recycling. These data led to a new model for A-RAF function in endocytic trafficking.

  4. Modulation of Endocytic Traffic in Polarized Madin-Darby Canine Kidney Cells by the Small GTPase RhoA

    Science.gov (United States)

    Leung, Som-Ming; Rojas, Raul; Maples, Christopher; Flynn, Christopher; Ruiz, Wily G.; Jou, Tzuu-Shuh; Apodaca, Gerard

    1999-01-01

    Efficient postendocytic membrane traffic in polarized epithelial cells is thought to be regulated in part by the actin cytoskeleton. RhoA modulates assemblies of actin in the cell, and it has been shown to regulate pinocytosis and phagocytosis; however, its effects on postendocytic traffic are largely unexplored. To this end, we expressed wild-type RhoA (RhoAWT), dominant active RhoA (RhoAV14), and dominant inactive RhoA (RhoAN19) in Madin-Darby canine kidney (MDCK) cells expressing the polymeric immunoglobulin receptor. RhoAV14 expression stimulated the rate of apical and basolateral endocytosis, whereas RhoAN19 expression decreased the rate from both membrane domains. Polarized basolateral recycling of transferrin was disrupted in RhoAV14-expressing cells as a result of increased ligand release at the apical pole of the cell. Degradation of basolaterally internalized epidermal growth factor was slowed in RhoAV14-expressing cells. Although apical recycling of immunoglobulin A (IgA) was largely unaffected in cells expressing RhoAV14, transcytosis of basolaterally internalized IgA was severely impaired. Morphological and biochemical analyses demonstrated that a large proportion of IgA internalized from the basolateral pole of RhoAV14-expressing cells remained within basolateral early endosomes and was slow to exit these compartments. RhoAN19 and RhoAWT expression had little effect on these postendocytic pathways. These results indicate that in polarized MDCK cells activated RhoA may modulate endocytosis from both membrane domains and postendocytic traffic at the basolateral pole of the cell. PMID:10588664

  5. Properties of train traffic flow in a moving block system

    Institute of Scientific and Technical Information of China (English)

    Wang Min; Zeng Jun-Wei; Qian Yong-Sheng; Li Wen-Jun; Yang Fang; Jia Xin-Xin

    2012-01-01

    The development direction of railways is toward the improvement of capacity and service quality,where the service quality includes safety,schedule,high speed,and comfort.In light of the existing cellular automaton models,in this paper,we develop a model to analyze the mixed running processes of trains with maximal speeds of 500 km/h and 350 km/h respectively in the moving block system.In the proposed model,we establish some sound rules to control the running processes of a train,where the rules include the departure rules in the intermediate stations,the overtaking rules,and the conditions of speed limitation for a train stopping at a station or passing through a station.With the consideration of the mixed ratio and the distance between two adjacent stations,the properties of the train traffic flow (including capacity and average speed) are simulated.The numerical results show that the interactions among different trains will affect the capacity,and a proper increase of the spatial distance between two adjacent stations can enhance the capacity and the average speed under the moving block.

  6. Traffic Modelling for Moving-Block Train Control System

    Institute of Scientific and Technical Information of China (English)

    TANG Tao; LI Ke-Ping

    2007-01-01

    This paper presents a new cellular automaton (CA) model for train control system simulation.In the proposed CA model,the driver reactions to train movements are captured by some updated rules.The space-time diagram of traffic flow and the trajectory of train movement is used to obtain insight into the characteristic behavior of railway traffic flow.A number of simulation results demonstrate that the proposed CA model can be successfully used for the simulations of railway traffic.Not only the characteristic behavior of railway traffic flow can be reproduced,but also the simulation values of the minimum time headway are close to the theoretical values.

  7. Effects of PDT on the endocytic pathway

    Science.gov (United States)

    Kessel, David

    2010-02-01

    Two lines of evidence point to an early effect of photodamage on membrane trafficking. [1] Internalization of a fluorescent probe for hydrophobic membrane loci was impaired by prior photodamage. [2] Interference with the endocytic pathway by the PI-3 kinase antagonist wortmannin led to accumulation of cytoplasmic vacuoles suggesting a block in the recycling of plasma membrane components. Prior photodamage blocked this pathway so that no vacuoles were formed upon exposure of cells to wortmannin. In a murine hepatoma line, the endocytic pathway was preferentially sensitive to lysosomal photodamage. The role of photodamage to the endocytic pathway as a factor in PDT efficacy remains to be assessed.

  8. Development of traffic induced permanent strain in concrete block pavements

    NARCIS (Netherlands)

    Huurman, M.

    1996-01-01

    Concrete block pavements (c.b.p.) commonly consist of concrete blocks placed over a granular substructure. As a result of wheel load passages permanent strains will slowly develop in the substructure and cause rutting. This paper is about the prediction of the permanent strain development in the sub

  9. Development of traffic induced permanent strain in concrete block pavements

    OpenAIRE

    1996-01-01

    Concrete block pavements (c.b.p.) commonly consist of concrete blocks placed over a granular substructure. As a result of wheel load passages permanent strains will slowly develop in the substructure and cause rutting. This paper is about the prediction of the permanent strain development in the substructure and the associated rutting on the basis of the results of repeated load triaxial tests and analytical models. By discussing the behaviour of three c.b.p.'s it is shown that insight into c...

  10. Spring-block model for a single-lane highway traffic

    CERN Document Server

    Jarai-Szabo, Ferenc; Neda, Zoltan

    2010-01-01

    A simple one-dimensional spring-block chain with asymmetric interactions is considered to model an idealized single-lane highway traffic. The main elements of the system are blocks (modeling cars), springs with unidirectional interactions (modeling distance keeping interactions between neighbors), static and kinetic friction (modeling inertia of drivers and cars) and spatiotemporal disorder in the values of these friction forces (modeling differences in the driving attitudes). The traveling chain of cars correspond to the dragged spring-block system. Our statistical analysis for the spring-block chain predicts a non-trivial and rich complex behavior. As a function of the disorder level in the system a dynamic phase-transition is observed. For low disorder levels uncorrelated slidings of blocks are revealed while for high disorder levels correlated avalanches dominates.

  11. 49 CFR 236.401 - Automatic block signal system and interlocking standards applicable to traffic control systems.

    Science.gov (United States)

    2010-10-01

    ... TRAIN CONTROL SYSTEMS, DEVICES, AND APPLIANCES Traffic Control Systems Standards § 236.401 Automatic... 49 Transportation 4 2010-10-01 2010-10-01 false Automatic block signal system and interlocking standards applicable to traffic control systems. 236.401 Section 236.401 Transportation Other...

  12. Deployment of Palmic Concrete Pavement Blocks in Light and Heavy Traffic Situations

    Directory of Open Access Journals (Sweden)

    Eric Ababio Ohemeng

    2015-04-01

    Full Text Available The main objective of this research was to investigate the viability of deploying palmic concrete pavement blocks (PCPBs in light and heavy traffic situations. The term “palmic concrete” refers to any concrete containing palm kernel shell and ordinary aggregates. In this study cement, fine aggregate, coarse aggregate and palm kernel shell (PKS were used. It was observed that density and strengths of the PCPBs decreased as the PKS content increased. Although, the strengths of the PCPBs lowered as the percentage of PKS increased, compressive strengths of 30.00 N/mm2 to 48.70 N/mm2 which are satisfactory for light traffic and heavy traffic situations could be achieved if 0% to 30% PKS contents are used. A model was also developed to predict the density of PCPBs through laboratory analysis. The model is only capable of predicting the density of palmic concrete products if the water cement ratio, the curing age, the aggregates cement ratio and the curing condition used are within the tested range.

  13. Endocytic collagen degradation

    DEFF Research Database (Denmark)

    Madsen, Daniel H.; Jürgensen, Henrik J.; Ingvarsen, Signe;

    2012-01-01

    it crucially important to understand both the collagen synthesis and turnover mechanisms in this condition. Here we show that the endocytic collagen receptor, uPARAP/Endo180, is a major determinant in governing the balance between collagen deposition and degradation. Cirrhotic human livers displayed a marked......Fibrosis of the liver and its end-stage, cirrhosis, represent major health problems worldwide. In these fibrotic conditions, activated fibroblasts and hepatic stellate cells display a net deposition of collagen. This collagen deposition is a major factor leading to liver dysfunction, thus making...... up-regulation of uPARAP/Endo180 in activated fibroblasts and hepatic stellate cells located close to the collagen deposits. In a hepatic stellate cell line, uPARAP/Endo180 was shown to be active in, and required for, the uptake and intracellular degradation of collagen. To evaluate the functional...

  14. Numb is an endocytic protein

    DEFF Research Database (Denmark)

    Santolini, E; Puri, C; Salcini, A E;

    2000-01-01

    Numb is a protein that in Drosophila determines cell fate as a result of its asymmetric partitioning at mitosis. The function of Numb has been linked to its ability to bind and to biologically antagonize Notch, a membrane receptor that also specifies cell fate. The biochemical mechanisms underlying......15, a component of the endocytic machinery. Here, we demonstrate that Numb is an endocytic protein. We found that Numb localizes to endocytic organelles and is cotrafficked with internalizing receptors. Moreover, it associates with the appendage domain of alpha adaptin, a subunit of AP2, a major...

  15. Release of canine parvovirus from endocytic vesicles.

    Science.gov (United States)

    Suikkanen, Sanna; Antila, Mia; Jaatinen, Anne; Vihinen-Ranta, Maija; Vuento, Matti

    2003-11-25

    Canine parvovirus (CPV) is a small nonenveloped virus with a single-stranded DNA genome. CPV enters cells by clathrin-mediated endocytosis and requires an acidic endosomal step for productive infection. Virion contains a potential nuclear localization signal as well as a phospholipase A(2) like domain in N-terminus of VP1. In this study we characterized the role of PLA(2) activity on CPV entry process. PLA(2) activity of CPV capsids was triggered in vitro by heat or acidic pH. PLA(2) inhibitors inhibited the viral proliferation suggesting that PLA(2) activity is needed for productive infection. The N-terminus of VP1 was exposed during the entry, suggesting that PLA(2) activity might have a role during endocytic entry. The presence of drugs modifying endocytosis (amiloride, bafilomycin A(1), brefeldin A, and monensin) caused viral proteins to remain in endosomal/lysosomal vesicles, even though the drugs were not able to inhibit the exposure of VP1 N-terminal end. These results indicate that the exposure of N-terminus of VP1 alone is not sufficient to allow CPV to proliferate. Some other pH-dependent changes are needed for productive infection. In addition to blocking endocytic entry, amiloride was able to block some postendocytic steps. The ability of CPV to permeabilize endosomal membranes was demonstrated by feeding cells with differently sized rhodamine-conjugated dextrans together with the CPV in the presence or in the absence of amiloride, bafilomycin A(1), brefeldin A, or monensin. Dextran with a molecular weight of 3000 was released from vesicles after 8 h of infection, while dextran with a molecular weight of 10,000 was mainly retained in vesicles. The results suggest that CPV infection does not cause disruption of endosomal vesicles. However, the permeability of endosomal membranes apparently changes during CPV infection, probably due to the PLA(2) activity of the virus. These results suggest that parvoviral PLA(2) activity is essential for productive

  16. Aberrant trafficking of NSCLC-associated EGFR mutants through the endocytic recycling pathway promotes interaction with Src@

    Directory of Open Access Journals (Sweden)

    Band Vimla

    2009-11-01

    Full Text Available Abstract Background Epidermal growth factor receptor (EGFR controls a wide range of cellular processes, and altered EGFR signaling contributes to human cancer. EGFR kinase domain mutants found in non-small cell lung cancer (NSCLC are constitutively active, a trait critical for cell transformation through activation of downstream pathways. Endocytic trafficking of EGFR is a major regulatory mechanism as ligand-induced lysosomal degradation results in termination of signaling. While numerous studies have examined mutant EGFR signaling, the endocytic traffic of mutant EGFR within the NSCLC milieu remains less clear. Results This study shows that mutant EGFRs in NSCLC cell lines are constitutively endocytosed as shown by their colocalization with the early/recycling endosomal marker transferrin and the late endosomal/lysosomal marker LAMP1. Notably, mutant EGFRs, but not the wild-type EGFR, show a perinuclear accumulation and colocalization with recycling endosomal markers such as Rab11 and EHD1 upon treatment of cells with endocytic recycling inhibitor monensin, suggesting that mutant EGFRs preferentially traffic through the endocytic recycling compartments. Importantly, monensin treatment enhanced the mutant EGFR association and colocalization with Src, indicating that aberrant transit through the endocytic recycling compartment promotes mutant EGFR-Src association. Conclusion The findings presented in this study show that mutant EGFRs undergo aberrant traffic into the endocytic recycling compartment which allows mutant EGFRs to engage in a preferential interaction with Src, a critical partner for EGFR-mediated oncogenesis.

  17. 呼叫中心分块回归话务量预测%Block regression traffic prediction model for call center

    Institute of Scientific and Technical Information of China (English)

    张沪寅; 胡瑞芸; 何政

    2016-01-01

    为获得前瞻性话务量数据,解决呼叫中心坐席安排的问题,实现人力资源合理配置,分析历史话务量特性,提出了基于支持向量机和K 近邻算法的分块回归(SKBR)话务量预测模型。将话务量按日期类型分为工作日话务量、周末话务量以及节假日话务量,采用不同的模型预测相应的话务量。以某省电力呼叫中心话务量为例,在Matlab平台上进行实验。结果证明,相比SVM模型和改进寻参方法的SVM模型,SKBR模型在预测准确性上有所提升。%In order to obtain the prospective traffic data, solve the seats arrangement problem of call center, realize the rational allocation of human resources, block regression traffic prediction model, based on support vector machine and K-nearest neighbor algorithm is proposed(SKBR), after analyzing the characteristics of historical traffic data. According to the date type, traffic can be divided into weekday traffic, weekend traffic and holiday traffic, and different model is used to predict the corresponding traffic. Taking the traffic of a province electric power call center for example, experiments are carried on the MATLAB platform. Results show that compared with the SVM model and improved SVM model for its method of searching parameters, SKBR model has improved the prediction accuracy.

  18. Anti-α4 antibody treatment blocks virus traffic to the brain and gut early, and stabilizes CNS injury late in infection.

    Directory of Open Access Journals (Sweden)

    Jennifer H Campbell

    2014-12-01

    Full Text Available Four SIV-infected monkeys with high plasma virus and CNS injury were treated with an anti-α4 blocking antibody (natalizumab once a week for three weeks beginning on 28 days post-infection (late. Infection in the brain and gut were quantified, and neuronal injury in the CNS was assessed by MR spectroscopy, and compared to controls with AIDS and SIV encephalitis. Treatment resulted in stabilization of ongoing neuronal injury (NAA/Cr by 1H MRS, and decreased numbers of monocytes/macrophages and productive infection (SIV p28+, RNA+ in brain and gut. Antibody treatment of six SIV infected monkeys at the time of infection (early for 3 weeks blocked monocyte/macrophage traffic and infection in the CNS, and significantly decreased leukocyte traffic and infection in the gut. SIV - RNA and p28 was absent in the CNS and the gut. SIV DNA was undetectable in brains of five of six early treated macaques, but proviral DNA in guts of treated and control animals was equivalent. Early treated animals had low-to-no plasma LPS and sCD163. These results support the notion that monocyte/macrophage traffic late in infection drives neuronal injury and maintains CNS viral reservoirs and lesions. Leukocyte traffic early in infection seeds the CNS with virus and contributes to productive infection in the gut. Leukocyte traffic early contributes to gut pathology, bacterial translocation, and activation of innate immunity.

  19. Endocytic pathways mediating oligomeric Aβ42 neurotoxicity

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    Laxton Kevin

    2010-05-01

    Full Text Available Abstract Background One pathological hallmark of Alzheimer's disease (AD is amyloid plaques, composed primarily of amyloid-β peptide (Aβ. Over-production or diminished clearance of the 42 amino acid form of Aβ (Aβ42 in the brain leads to accumulation of soluble Aβ and plaque formation. Soluble oligomeric Aβ (oAβ has recently emerged to be as a likely proximal cause of AD. Results Here we demonstrate that endocytosis is critical in mediating oAβ42-induced neurotoxicity and intraneuronal accumulation of Aβ. Inhibition of clathrin function either with a pharmacological inhibitor, knock-down of clathrin heavy chain expression, or expression of the dominant-negative mutant of clathrin-assembly protein AP180 did not block oAβ42-induced neurotoxicity or intraneuronal accumulation of Aβ. However, inhibition of dynamin and RhoA by expression of dominant negative mutants reduced neurotoxicity and intraneuronal Aβ accumulation. Pharmacologic inhibition of the dynamin-mediated endocytic pathway by genistein also reduced neurotoxicity. Conclusions These data suggest that dynamin-mediated and RhoA-regulated endocytosis are integral steps for oligomeric Aβ42-induced neurotoxicity and intraneuronal Aβ accumulation.

  20. Traffic block port management platform based on B/S mode%基于B/S模式的交通卡口管理平台

    Institute of Scientific and Technical Information of China (English)

    沈星彤; 黄小莉

    2012-01-01

    采用B/S模式设计了一个交通卡口管理平台,依托卡口系统现有数据网络对监控图像进行网络传送,采用视频监控技术实现信息共享、远程监控和管理,能够为远端监控人员掌握第一手现场资料、做出正确决策提供强大的支持。%This paper designs of a traffic block port management platform based on the B/S mode. The monitoring image relies on the existing data network of traffic block port system for network transmission. This platform introduces video surveillance tech- nology to achieve information sharing, remote monitoring and management. It can provide extremely strong support for the remote monitoring personnel's first-hand field data and making the right decisions.

  1. Internalization of Trichosanthin via Different Endocytic Mechanisms

    Institute of Scientific and Technical Information of China (English)

    ZHANG Fan; SUI Senfang

    2005-01-01

    Trichosanthin (TCS) is a plant toxin with ribosome-inactivating activity. TCS can be internalized by the host cells and then attacks the ribosomes resulting in cell death. However, the manner for endocytic uptake of TCS is not well understood. The present work investigates the endocytosis pathway of TCS in human choriocarcinoma cells. The different endocytic mechanisms are interfered by potassium depletion, cholesterol-extraction/addition, or treatments of various drugs. The experiments detect their effects on the TCS-uptake. The results show that a large portion of the TCS can be internalized by clathrin-dependent, as well as by clathrin-independent but cholesterol-dependent endocytosis in human choriocarcinoma cells.

  2. Human immunodeficiency virus type 1 endocytic trafficking through macrophage bridging conduits facilitates spread of infection.

    Science.gov (United States)

    Kadiu, Irena; Gendelman, Howard E

    2011-12-01

    Bridging conduits (BC) sustain communication and homeostasis between distant tethered cells. These are also exploited commonly for direct cell-to-cell transfer of microbial agents. Conduits efficiently spread infection, effectively, at speeds faster than fluid phase exchange while shielding the microbe against otherwise effective humoral immunity. Our laboratory has sought to uncover the mechanism(s) for these events for human immunodeficiency virus type one (HIV-1) infection. Indeed, in our prior works HIV-1 Env and Gag antigen and fluorescent virus tracking were shown sequestered into endoplasmic reticulum-Golgi organelles but the outcomes for spreading viral infection remained poorly defined. Herein, we show that HIV-1 specifically traffics through endocytic compartments contained within BC and directing such macrophage-to-macrophage viral transfers. Following clathrin-dependent viral entry, HIV-1 constituents bypass degradation by differential sorting from early to Rab11(+) recycling endosomes and multivesicular bodies. Virus-containing endocytic viral cargoes propelled by myosin II through BC spread to neighboring uninfected cells. Disruption of endosomal motility with cytochalasin D, nocodasole and blebbistatin diminish intercellular viral spread. These data lead us to propose that HIV-1 hijacks macrophage endocytic and cytoskeletal machineries for high-speed cell-to-cell spread.

  3. The RCP-Rab11 complex regulates endocytic protein sorting.

    Science.gov (United States)

    Peden, Andrew A; Schonteich, Eric; Chun, John; Junutula, Jagath R; Scheller, Richard H; Prekeris, Rytis

    2004-08-01

    Rab 11 GTPase is an important regulator of endocytic membrane traffic. Recently, we and others have identified a novel family of Rab11 binding proteins, known as Rab11-family interacting proteins (FIPs). One of the family members, Rab coupling protein (RCP), was identified as a protein binding to both Rab4 and Rab11 GTPases. RCP was therefore suggested to serve a dual function as Rab4 and Rab11 binding protein. In this study, we characterized the cellular functions of RCP and mapped its interactions with Rab4 and Rab11. Our data show that RCP interacts only weakly with Rab4 in vitro and does not play the role of coupling Rab11 and Rab4 in vivo. Furthermore, our data indicate that the RCP-Rab11 complex regulates the sorting of transferrin receptors from the degradative to the recycling pathway. We therefore propose that RCP functions primarily as a Rab11 binding protein that regulates protein sorting in tubular endosomes.

  4. Zitongdong Block

    Institute of Scientific and Technical Information of China (English)

    1996-01-01

    @@ The Zitongdong Block (Eastern Zitong Block) is located in the northwest of the Sichuan Basin. Tectonically, it is situated in the east part of Zitong Depression, southeast of mid-Longmenshan folded and faulted belt( as shown on Fig. 8 ), covering an area of 1 730 km2. The traffic is very convenient, the No. 108 national highway passes through the north of the block. Topographically, the area belongs to low hilly land at the elevation of 500-700 m.

  5. Effector-triggered immunity blocks pathogen degradation of an immunity-associated vesicle traffic regulator in Arabidopsis.

    Science.gov (United States)

    Nomura, Kinya; Mecey, Christy; Lee, Young-Nam; Imboden, Lori Alice; Chang, Jeff H; He, Sheng Yang

    2011-06-28

    Innate immunity in plants can be triggered by microbe- and pathogen-associated molecular patterns. The pathogen-associated molecular pattern-triggered immunity (PTI) is often suppressed by pathogen effectors delivered into the host cell. Plants can overcome pathogen suppression of PTI and reestablish pathogen resistance through effector-triggered immunity (ETI). An unanswered question is how plants might overcome pathogen-suppression of PTI during ETI. Findings described in this paper suggest a possible mechanism. During Pseudomonas syringae pathovar tomato (Pst) DC3000 infection of Arabidopsis, a host ADP ribosylation factor guanine nucleotide exchange factor, AtMIN7, is destabilized by the pathogen effector HopM1 through the host 26S proteasome. In this study, we discovered that AtMIN7 is required for not only PTI, consistent with the notion that Pst DC3000 degrades AtMIN7 to suppress PTI, but also ETI. The AtMIN7 level in healthy plants is low, but increases posttranscriptionally in response to activation of PTI. Whereas DC3000 infection led to degradation of AtMIN7, activation of ETI by three different effectors, AvrRpt2, AvrPphB, and HopA1, in Col-0 plants blocks the ability of Pst DC3000 to destabilize AtMIN7. Further analyses of bacterial translocation of HopM1 and AtMIN7 stability in HopM1 transgenic plants show that ETI prevents HopM1-mediated degradation of AtMIN7 inside the plant cell. Both AtMIN7 and HopM1 are localized to the trans-Golgi network/early endosome, a subcellular compartment that is not previously known to be associated with bacterial pathogenesis in plants. Thus, blocking pathogen degradation of trans-Golgi network/early endosome-associated AtMIN7 is a critical part of the ETI mechanism to counter bacterial suppression of PTI.

  6. Longmenshan Block

    Institute of Scientific and Technical Information of China (English)

    1996-01-01

    @@ Longmenshan Block is located in Jiange County of Jiangyou City in the northwest of Sichuan Basin. covering an area of 2 628 km2. Geologically, it is situated in the Mid-Longmenshan fault and fold belt, neighbouring Zitong Depression in its southeast. There are mountains surrounding its northwest , the rest area being hilly land,with the elevation of 500-700 m. The BaoCheng railway and the No. 108 highway run through the block, the traffic is very convenient.

  7. The HPV16 E5 oncogene inhibits endocytic trafficking

    DEFF Research Database (Denmark)

    Thomsen, P; van Deurs, B; Norrild, B

    2000-01-01

    The small hydrophobic E5 protein of Human Papillomavirus type 16 (HPV16) binds to the 16-kDa subunit of the V-H+-ATPase. This binding has been suggested to interfere with acidification of late endocytic structures. We here used video microscopy, ratio imaging and confocal microscopy of living C127...... perturbs trafficking from early to late endocytic structures rather than acidification....

  8. Endocytic recycling protein EHD1 regulates primary cilia morphogenesis and SHH signaling during neural tube development.

    Science.gov (United States)

    Bhattacharyya, Sohinee; Rainey, Mark A; Arya, Priyanka; Dutta, Samikshan; George, Manju; Storck, Matthew D; McComb, Rodney D; Muirhead, David; Todd, Gordon L; Gould, Karen; Datta, Kaustubh; Gelineau-van Waes, Janee; Band, Vimla; Band, Hamid

    2016-02-17

    Members of the four-member C-terminal EPS15-Homology Domain-containing (EHD) protein family play crucial roles in endocytic recycling of cell surface receptors from endosomes to the plasma membrane. In this study, we show that Ehd1 gene knockout in mice on a predominantly B6 background is embryonic lethal. Ehd1-null embryos die at mid-gestation with a failure to complete key developmental processes including neural tube closure, axial turning and patterning of the neural tube. We found that Ehd1-null embryos display short and stubby cilia on the developing neuroepithelium at embryonic day 9.5 (E9.5). Loss of EHD1 also deregulates the ciliary SHH signaling with Ehd1-null embryos displaying features indicative of increased SHH signaling, including a significant downregulation in the formation of the GLI3 repressor and increase in the ventral neuronal markers specified by SHH. Using Ehd1-null MEFS we found that EHD1 protein co-localizes with the SHH receptor Smoothened in the primary cilia upon ligand stimulation. Under the same conditions, EHD1 was shown to co-traffic with Smoothened into the developing primary cilia and we identify EHD1 as a direct binding partner of Smoothened. Overall, our studies identify the endocytic recycling regulator EHD1 as a novel regulator of the primary cilium-associated trafficking of Smoothened and Hedgehog signaling.

  9. Precise positioning of myosin VI on endocytic vesicles in vivo.

    Directory of Open Access Journals (Sweden)

    David Altman

    2007-08-01

    Full Text Available Myosin VI has been studied in both a monomeric and a dimeric form in vitro. Because the functional characteristics of the motor are dramatically different for these two forms, it is important to understand whether myosin VI heavy chains are brought together on endocytic vesicles. We have used fluorescence anisotropy measurements to detect fluorescence resonance energy transfer between identical fluorophores (homoFRET resulting from myosin VI heavy chains being brought into close proximity. We observed that, when associated with clathrin-mediated endocytic vesicles, myosin VI heavy chains are precisely positioned to bring their tail domains in close proximity. Our data show that on endocytic vesicles, myosin VI heavy chains are brought together in an orientation that previous in vitro studies have shown causes dimerization of the motor. Our results are therefore consistent with vesicle-associated myosin VI existing as a processive dimer, capable of its known trafficking function.

  10. Endocytic pathway mediates refractoriness of insect Bactrocera dorsalis to RNA interference.

    Science.gov (United States)

    Li, Xiaoxue; Dong, Xiaolong; Zou, Cong; Zhang, Hongyu

    2015-03-03

    RNA interference (RNAi) is a powerful and convenient tool for sequence-specific gene silencing, and it is triggered by double-stranded RNA (dsRNA). RNAi can be easily achieved in many eukaryotes by either injecting or feeding dsRNAs. This mechanism has demonstrated its potential in fundamental research on genetics, medicine and agriculture. However, the possibility that insects might develop refractoriness to RNAi remains unexplored. In this study, we report that the oriental fruit fly, Bactrocera dorsalis, became refractory to RNAi using orally administered dsRNA targeting endogenous genes. Furthermore, refractoriness to RNAi is not gene-specific, and its duration depends on the dsRNA concentration. RNAi blockage requires the endocytic pathway. Fluorescence microscopy indicated that in RNAi refractory flies, dsRNA uptake is blocked. Genes involved in the entry of dsRNAs into cells, including chc, cog3, light and others, are down-regulated in RNAi refractory flies. Increasing the endocytic capacity by improving F-actin polymerization disrupts RNAi refractoriness after both primary and secondary dsRNA exposures. Our results demonstrate that an insect can become refractory to RNAi by preventing the entry of dsRNA into its cells.

  11. Chadong Block

    Institute of Scientific and Technical Information of China (English)

    1996-01-01

    @@ The Chadong Block, located in the east of Qaidam Basin, Qinghai Province, covers an area of 12 452 km2. It is bounded by Kunlum Mountains in the south and the northwest is closely adjacent to Aimunike Mountain.Rivers are widely distributed, which always run in NWSE direction, including the Sulunguole, Qaidam and Haluwusu Rivers. The traffic condition is good, the Qinghai-Tibet highway stretching through the whole area and the Lan-Qing railway, 20-50 km away from the block, passing from north to west. A lot of Mongolia minority people have settled there, of which herdsmen always live nearby the Qaidam River drainage area.

  12. Chengzikou Block

    Institute of Scientific and Technical Information of China (English)

    1996-01-01

    @@ Chengzikou Block is located in the north of Hekou district, Dongying City, Shandong Province, adjacent to Bohai Bay. It can be geographically divided into three units: onshore, transitional zone and offshore ultrashallow zone, totally covering an area of 470 km2. The southern onshore area is low and gentle in topography;the northern shallow sea is at water depths of 2-8 m below sea level, and the transitional zone occupies more than 60% of the whole block. The climate belongs to temperate zone with seasonal wind. Highways are welldeveloped here, and the traffic is very convenient. The Chengzikou Block is about 80 km away from Dongying City and 290 km from Jinan City in the south. The northern offshore area of the block is 160 km away from Longkou port in the east and only 38 km away in the west from Zhuangxi port.

  13. Endocytic regulation of TGF-β signaling

    Institute of Scientific and Technical Information of China (English)

    Ye-Guang Chen

    2009-01-01

    Transforming growth factor-β(TGF-β)signaling is tightly regulated to ensure its proper physiological functions in diflferent cells and tissues.Like other cell surface receptors,TGF-β receptors are internalized into the cell,and this process plays an important regulatory role in TGF-βsignaling.It is well documented that TGF-β receptors are en-docytosed via clathrin-coated vesicles as TGF-β endocytosis can be blocked by potassium depletion and the GTPase-deficient dynamin K44A mutant.TGF-β receptors may also enter cells via cholesterol-rich membrane microdomain lipid rafts/caveolae and are found in caveolin-1-positive vesicles.Although receptor endocytosis is not essential for TGF-β signaling,clathrin-mediated endocytosis has been shown to promote TGF-β-induced Smad activation and transcriptional responses.Lipid rafts/caveolae are widely regarded as signaling centers for G protein-coupled recep-tors and tyrosine kinase receptors,but they are indicated to facilitate the degradation of TGF-β receptors and there-fore turnoff of TGF-β signaling.This review summarizes current understanding of TGF-β receptor endocytosis,thepossible mechanisms underlying this process,and the role of endocytosis in modulation of TGF-β signaling.

  14. Important relationships between Rab and MICAL proteins in endocytic trafficking

    Institute of Scientific and Technical Information of China (English)

    Juliati; Rahajeng; Sai; Srinivas; Panapakkam; Giridharan; Naava; Naslavsky; Steve; Caplan

    2010-01-01

    The internalization of essential nutrients,lipids and receptors is a crucial process for all eukaryotic cells.Accordingly,endocytosis is highly conserved across cell types and species.Once internalized,small cargocontaining vesicles fuse with early endosomes(also known as sorting endosomes),where they undergo segregation to distinct membrane regions and are sorted and transported on through the endocytic pathway.Although the mechanisms that regulate this sorting are still poorly understood,some receptors are directed to late endosomes and lysosomes for degradation,whereas other receptors are recycled back to the plasma membrane;either directly or through recycling endosomes.The Rab family of small GTP-binding proteins plays crucial roles in regulating these trafficking pathways.Rabs cycle from inactive GDP-bound cytoplasmic proteins to active GTP-bound membraneassociated proteins,as a consequence of the activity of multiple specific GTPase-activating proteins(GAPs) and GTP exchange factors(GEFs).Once bound to GTP,Rabs interact with a multitude of effector proteins that carry out Rab-specific functions.Recent studies have shown that some of these effectors are also interaction partners for the C-terminal Eps15 homology(EHD) proteins,which are also intimately involved in endocytic regulation.A particularly interesting example of common Rab-EHD interaction partners is the MICALlike protein,MICAL-L1.MICAL-L1 and its homolog,MICAL-L2,belong to the larger MICAL family of proteins,and both have been directly implicated in regulating endocytic recycling of cell surface receptors and junctional proteins,as well as controlling cytoskeletal rearrangement and neurite outgrowth.In this review,we summarize the functional roles of MICAL and Rab proteins,and focus on the significance of their interactions and the implications for endocytic transport.

  15. Recombinant antibody mediated delivery of organelle-specific DNA pH sensors along endocytic pathways

    Science.gov (United States)

    Modi, Souvik; Halder, Saheli; Nizak, Clément; Krishnan, Yamuna

    2013-12-01

    DNA has been used to build nanomachines with potential in cellulo and in vivo applications. However their different in cellulo applications are limited by the lack of generalizable strategies to deliver them to precise intracellular locations. Here we describe a new molecular design of DNA pH sensors with response times that are nearly 20 fold faster. Further, by changing the sequence of the pH sensitive domain of the DNA sensor, we have been able to tune their pH sensitive regimes and create a family of DNA sensors spanning ranges from pH 4 to 7.6. To enable a generalizable targeting methodology, this new sensor design also incorporates a `handle' domain. We have identified, using a phage display screen, a set of three recombinant antibodies (scFv) that bind sequence specifically to the handle domain. Sequence analysis of these antibodies revealed several conserved residues that mediate specific interactions with the cognate DNA duplex. We also found that all three scFvs clustered into different branches indicating that their specificity arises from mutations in key residues. When one of these scFvs is fused to a membrane protein (furin) that traffics via the cell surface, the scFv-furin chimera binds the `handle' and ferries a family of DNA pH sensors along the furin endocytic pathway. Post endocytosis, all DNA nanodevices retain their functionality in cellulo and provide spatiotemporal pH maps of retrogradely trafficking furin inside living cells. This new molecular technology of DNA-scFv-protein chimeras can be used to site-specifically complex DNA nanostructures for bioanalytical applications.DNA has been used to build nanomachines with potential in cellulo and in vivo applications. However their different in cellulo applications are limited by the lack of generalizable strategies to deliver them to precise intracellular locations. Here we describe a new molecular design of DNA pH sensors with response times that are nearly 20 fold faster. Further, by changing

  16. 14 CFR 298.61 - Reporting of traffic statistics.

    Science.gov (United States)

    2010-01-01

    ...: blocked-space agreements; part-charter agreements; code-sharing agreements; wet-lease agreements, and... traffic moving under these agreements is reported on Schedule T-100 the same way as any other traffic...

  17. Traffic Sign Recognition System based on Cambridge Correlator Image Comparator

    OpenAIRE

    J. Turan; L. Ovsenik; T. Harasthy

    2012-01-01

    Paper presents basic information about application of Optical Correlator (OC), specifically Cambridge Correlator, in system to recognize of traffic sign. Traffic Sign Recognition System consists of three main blocks, Preprocessing, Optical Correlator and Traffic Sign Identification. The Region of Interest (ROI) is defined and chosen in preprocessing block and then goes to Optical Correlator, where is compared with database of Traffic Sign. Output of Optical Correlation is correlation plane, w...

  18. Traffic Monitor

    Science.gov (United States)

    1992-01-01

    Mestech's X-15 "Eye in the Sky," a traffic monitoring system, incorporates NASA imaging and robotic vision technology. A camera or "sensor box" is mounted in a housing. The sensor detects vehicles approaching an intersection and sends the information to a computer, which controls the traffic light according to the traffic rate. Jet Propulsion Laboratory technical support packages aided in the company's development of the system. The X-15's "smart highway" can also be used to count vehicles on a highway and compute the number in each lane and their speeds, important information for freeway control engineers. Additional applications are in airport and railroad operations. The system is intended to replace loop-type traffic detectors.

  19. Megalin and cubilin: synergistic endocytic receptors in renal proximal tubule.

    Science.gov (United States)

    Christensen, E I; Birn, H

    2001-04-01

    The multiligand, endocytic receptors megalin and cubilin are colocalized in the renal proximal tubule. They are heavily expressed in the apical endocytic apparatus. Megalin is a 600-kDa transmembrane protein belonging to the low-density lipoprotein-receptor family. The cytoplasmic tail contains three NPXY motifs that mediate the clustering in coated pits and are possibly involved in signaling functions. Cubilin, also known as the intestinal intrinsic factor-cobalamin receptor, is a 460-kDa receptor with no transmembrane domain and no known signal for endocytosis. Because the two receptors bind each other with high affinity and colocalize in several tissues, it is highly conceivable that megalin mediates internalization of cubilin and its ligands. Both receptors are important for normal tubular reabsorption of proteins, including albumin. Among the proteins normally filtered in the glomeruli, cubilin has been shown to bind albumin, immunoglobulin light chains, and apolipoprotein A-I. The variety of filtered ligands identified for megalin include vitamin-binding proteins, hormones, enzymes, apolipoprotein H, albumin, and beta(2)- and alpha(1)-microglobulin. Loss of these proteins and vitamins in the urine of megalin-deficient mice illustrates the physiological importance of this receptor.

  20. Matrix stiffness affects endocytic uptake of MK2-inhibitor peptides.

    Directory of Open Access Journals (Sweden)

    Jamie L Brugnano

    Full Text Available In this study, the role of substrate stiffness on the endocytic uptake of a cell-penetrating peptide was investigated. The cell-penetrating peptide, an inhibitor of mitogen-activated protein kinase activated protein kinase II (MK2, enters a primary mesothelial cell line predominantly through caveolae. Using tissue culture polystyrene and polyacrylamide gels of varying stiffness for cell culture, and flow cytometry quantification and enzyme-linked immunoassays (ELISA for uptake assays, we showed that the amount of uptake of the peptide is increased on soft substrates. Further, peptide uptake per cell increased at lower cell density. The improved uptake seen on soft substrates in vitro better correlates with in vivo functional studies where 10-100 µM concentrations of the MK2 inhibitor cell penetrating peptide demonstrated functional activity in several disease models. Additional characterization showed actin polymerization did not affect uptake, while microtubule polymerization had a profound effect on uptake. This work demonstrates that cell culture substrate stiffness can play a role in endocytic uptake, and may be an important consideration to improve correlations between in vitro and in vivo drug efficacy.

  1. Matrix stiffness affects endocytic uptake of MK2-inhibitor peptides.

    Science.gov (United States)

    Brugnano, Jamie L; Panitch, Alyssa

    2014-01-01

    In this study, the role of substrate stiffness on the endocytic uptake of a cell-penetrating peptide was investigated. The cell-penetrating peptide, an inhibitor of mitogen-activated protein kinase activated protein kinase II (MK2), enters a primary mesothelial cell line predominantly through caveolae. Using tissue culture polystyrene and polyacrylamide gels of varying stiffness for cell culture, and flow cytometry quantification and enzyme-linked immunoassays (ELISA) for uptake assays, we showed that the amount of uptake of the peptide is increased on soft substrates. Further, peptide uptake per cell increased at lower cell density. The improved uptake seen on soft substrates in vitro better correlates with in vivo functional studies where 10-100 µM concentrations of the MK2 inhibitor cell penetrating peptide demonstrated functional activity in several disease models. Additional characterization showed actin polymerization did not affect uptake, while microtubule polymerization had a profound effect on uptake. This work demonstrates that cell culture substrate stiffness can play a role in endocytic uptake, and may be an important consideration to improve correlations between in vitro and in vivo drug efficacy.

  2. Endophilin marks and controls a clathrin-independent endocytic pathway.

    Science.gov (United States)

    Boucrot, Emmanuel; Ferreira, Antonio P A; Almeida-Souza, Leonardo; Debard, Sylvain; Vallis, Yvonne; Howard, Gillian; Bertot, Laetitia; Sauvonnet, Nathalie; McMahon, Harvey T

    2015-01-22

    Endocytosis is required for internalization of micronutrients and turnover of membrane components. Endophilin has been assigned as a component of clathrin-mediated endocytosis. Here we show in mammalian cells that endophilin marks and controls a fast-acting tubulovesicular endocytic pathway that is independent of AP2 and clathrin, activated upon ligand binding to cargo receptors, inhibited by inhibitors of dynamin, Rac, phosphatidylinositol-3-OH kinase, PAK1 and actin polymerization, and activated upon Cdc42 inhibition. This pathway is prominent at the leading edges of cells where phosphatidylinositol-3,4-bisphosphate-produced by the dephosphorylation of phosphatidylinositol-3,4,5-triphosphate by SHIP1 and SHIP2-recruits lamellipodin, which in turn engages endophilin. This pathway mediates the ligand-triggered uptake of several G-protein-coupled receptors such as α2a- and β1-adrenergic, dopaminergic D3 and D4 receptors and muscarinic acetylcholine receptor 4, the receptor tyrosine kinases EGFR, HGFR, VEGFR, PDGFR, NGFR and IGF1R, as well as interleukin-2 receptor. We call this new endocytic route fast endophilin-mediated endocytosis (FEME).

  3. Ca/calmodulin-dependent phosphorylation of endocytic scaffold ITSN1

    Directory of Open Access Journals (Sweden)

    Morderer D. Ye.

    2014-01-01

    Full Text Available ITSN1 is an endocytic scaffold protein with a prominent function in synaptic transmission. It is known that Ca signaling is crucial for the regulation of synaptic proteins functioning. Aim. Checking the possibility of Ca/calmodulin-dependent phosphorylation of ITSN1. Methods. Affinity chromatography, in vitro kinase reaction, Western blotting, gel staining with fluorescent stains. Results. We show that the fraction of calmodulin-binding proteins is able to phosphorylate the recombinant fragments encoding the coiled-coil region and the SH3 domain-containing region of ITSN1 in the presence of Ca ions and calmodulin. Conclusions. The coiled-coil region and the SH3 domain-containing region of ITSN1 undergo Ca/calmodulin-dependent phosphorylation in vitro, suggesting a possible regulation of ITSN1 by Ca signaling.

  4. Proteomics of secretory and endocytic organelles in Giardia lamblia.

    Directory of Open Access Journals (Sweden)

    Petra B Wampfler

    Full Text Available Giardia lamblia is a flagellated protozoan enteroparasite transmitted as an environmentally resistant cyst. Trophozoites attach to the small intestine of vertebrate hosts and proliferate by binary fission. They access nutrients directly via uptake of bulk fluid phase material into specialized endocytic organelles termed peripheral vesicles (PVs, mainly on the exposed dorsal side. When trophozoites reach the G2/M restriction point in the cell cycle they can begin another round of cell division or encyst if they encounter specific environmental cues. They induce neogenesis of Golgi-like organelles, encystation-specific vesicles (ESVs, for regulated secretion of cyst wall material. PVs and ESVs are highly simplified and thus evolutionary diverged endocytic and exocytic organelle systems with key roles in proliferation and transmission to a new host, respectively. Both organelle systems physically and functionally intersect at the endoplasmic reticulum (ER which has catabolic as well as anabolic functions. However, the unusually high degree of sequence divergence in Giardia rapidly exhausts phylogenomic strategies to identify and characterize the molecular underpinnings of these streamlined organelles. To define the first proteome of ESVs and PVs we used a novel strategy combining flow cytometry-based organelle sorting with in silico filtration of mass spectrometry data. From the limited size datasets we retrieved many hypothetical but also known organelle-specific factors. In contrast to PVs, ESVs appear to maintain a strong physical and functional link to the ER including recruitment of ribosomes to organelle membranes. Overall the data provide further evidence for the formation of a cyst extracellular matrix with minimal complexity. The mass spectrometry proteomics data have been deposited to the ProteomeXchange Consortium with the dataset identifier PXD000694.

  5. Proteomics of secretory and endocytic organelles in Giardia lamblia.

    Science.gov (United States)

    Wampfler, Petra B; Tosevski, Vinko; Nanni, Paolo; Spycher, Cornelia; Hehl, Adrian B

    2014-01-01

    Giardia lamblia is a flagellated protozoan enteroparasite transmitted as an environmentally resistant cyst. Trophozoites attach to the small intestine of vertebrate hosts and proliferate by binary fission. They access nutrients directly via uptake of bulk fluid phase material into specialized endocytic organelles termed peripheral vesicles (PVs), mainly on the exposed dorsal side. When trophozoites reach the G2/M restriction point in the cell cycle they can begin another round of cell division or encyst if they encounter specific environmental cues. They induce neogenesis of Golgi-like organelles, encystation-specific vesicles (ESVs), for regulated secretion of cyst wall material. PVs and ESVs are highly simplified and thus evolutionary diverged endocytic and exocytic organelle systems with key roles in proliferation and transmission to a new host, respectively. Both organelle systems physically and functionally intersect at the endoplasmic reticulum (ER) which has catabolic as well as anabolic functions. However, the unusually high degree of sequence divergence in Giardia rapidly exhausts phylogenomic strategies to identify and characterize the molecular underpinnings of these streamlined organelles. To define the first proteome of ESVs and PVs we used a novel strategy combining flow cytometry-based organelle sorting with in silico filtration of mass spectrometry data. From the limited size datasets we retrieved many hypothetical but also known organelle-specific factors. In contrast to PVs, ESVs appear to maintain a strong physical and functional link to the ER including recruitment of ribosomes to organelle membranes. Overall the data provide further evidence for the formation of a cyst extracellular matrix with minimal complexity. The mass spectrometry proteomics data have been deposited to the ProteomeXchange Consortium with the dataset identifier PXD000694.

  6. Inactivation of Tor proteins affects the dynamics of endocytic proteins in early stage of endocytosis

    Indian Academy of Sciences (India)

    Brandon Tenay; Evin Kimberlin; Michelle Williams; Juliette Denise; Joshua Fakilahyel; Kyoungtae Kim

    2013-06-01

    Tor2 is an activator of the Rom2/Rho1 pathway that regulates -factor internalization. Since the recruitment of endocytic proteins such as actin-binding proteins and the amphiphysins precedes the internalization of -factor, we hypothesized that loss of Tor function leads to an alteration in the dynamics of the endocytic proteins. We report here that endocytic proteins, Abp1 and Rvs167, are less recruited to endocytic sites not only in tor2 but also tor1 mutants. Furthermore, we found that the endocytic proteins Rvs167 and Sjl2 are completely mistargeted to the cytoplasm in tor1tor2ts double mutant cells. We also demonstrate here that the efficiency of endocytic internalization or scission in all tor mutants was drastically decreased. In agreement with the Sjl2 mislocalization, we found that in tor1tor2ts double mutant cells, as well as other tor mutant cells, the overall PIP2 level was dramatically increased. Finally, the cell wall chitin content in tor2ts and tor1tor2ts mutant cells was also significantly increased. Taken together, both functional Tor proteins, Tor1 and Tor2, are essentially required for proper endocytic protein dynamics at the early stage of endocytosis.

  7. Renal thrombotic microangiopathy in mice with combined deletion of endocytic recycling regulators EHD3 and EHD4.

    Directory of Open Access Journals (Sweden)

    Manju George

    Full Text Available Eps15 Homology Domain-containing 3 (EHD3, a member of the EHD protein family that regulates endocytic recycling, is the first protein reported to be specifically expressed in the glomerular endothelium in the kidney; therefore we generated Ehd3(-/- mice and assessed renal development and pathology. Ehd3(-/- animals showed no overt defects, and exhibited no proteinuria or glomerular pathology. However, as the expression of EHD4, a related family member, was elevated in the glomerular endothelium of Ehd3(-/- mice and suggested functional compensation, we generated and analyzed Ehd3(-/-; Ehd4(-/- mice. These mice were smaller, possessed smaller and paler kidneys, were proteinuric and died between 3-24 weeks of age. Detailed analyses of Ehd3(-/-; Ehd4(-/- kidneys demonstrated thrombotic microangiopathy (TMA-like glomerular lesions including thickening and duplication of glomerular basement membrane, endothelial swelling and loss of fenestrations. Other changes included segmental podocyte foot process effacement, mesangial interposition, and abnormal podocytic and mesangial marker expression. The glomerular lesions observed were strikingly similar to those seen in human pre-eclampsia and mouse models of reduced VEGF expression. As altered glomerular endothelial VEGFR2 expression and localization and increased apoptosis was observed in the absence of EHD3 and EHD4, we propose that EHD-mediated endocytic traffic of key surface receptors such as VEGFR2 is essential for physiological control of glomerular function. Furthermore, Ehd3(-/-; Ehd4(-/- mice provide a unique model to elucidate mechanisms of glomerular endothelial injury which is observed in a wide variety of human renal and extra-renal diseases.

  8. α1A-adrenergic receptor induces activation of extracellular signal-regulated kinase 1/2 through endocytic pathway.

    Directory of Open Access Journals (Sweden)

    Fei Liu

    Full Text Available G protein-coupled receptors (GPCRs activate mitogen-activated protein kinases through a number of distinct pathways in cells. Increasing evidence has suggested that endosomal signaling has an important role in receptor signal transduction. Here we investigated the involvement of endocytosis in α(1A-adrenergic receptor (α(1A-AR-induced activation of extracellular signal-regulated kinase 1/2 (ERK1/2. Agonist-mediated endocytic traffic of α(1A-AR was assessed by real-time imaging of living, stably transfected human embryonic kidney 293A cells (HEK-293A. α(1A-AR was internalized dynamically in cells with agonist stimulation, and actin filaments regulated the initial trafficking of α(1A-AR. α(1A-AR-induced activation of ERK1/2 but not p38 MAPK was sensitive to disruption of endocytosis, as demonstrated by 4°C chilling, dynamin mutation and treatment with cytochalasin D (actin depolymerizing agent. Activation of protein kinase C (PKC and C-Raf by α(1A-AR was not affected by 4°C chilling or cytochalasin D treatment. U73122 (a phospholipase C [PLC] inhibitor and Ro 31-8220 (a PKC inhibitor inhibited α(1B-AR- but not α(1A-AR-induced ERK1/2 activation. These data suggest that the endocytic pathway is involved in α(1A-AR-induced ERK1/2 activation, which is independent of G(q/PLC/PKC signaling.

  9. Revolutionizing Technology Prioritizing Emergency Vehicles in Traffic

    Directory of Open Access Journals (Sweden)

    B. Ramya Sundaram

    2014-08-01

    Full Text Available The following study brings to the fore a technology exclusively engineered to eradicate the snags and inadequacies persisting in the existing system of traffic control and thus aims to provide a sophisticated operation and uninterrupted course for the emergency vehicles. The traffic in Indian roads has shown a tremendous rise over the years and the conventional pre-timed traffic signals and frequent traffic congestions, delaying the road commuters, have led to outrage and chaos on several occasions. This traffic management system has proved a nuisance for the effective operation of emergency vehicles, with the public adding to the woes, as each driver races along with the signal, unmindful of the emergency vehicles. A life threatening scenario ensues when emergency vehicles get blocked by the traffic, due to inefficient traffic management system and subsequently these emergency vehicles get delayed. The existing system requires manual interruption to guide other vehicles when an emergency vehicle passes through traffic junctions. It is therefore essential to implement a self-regulating and automated system in the traffic signals to facilitate smooth movement of emergency vehicles without posing a danger to other vehicles. The innovative traffic control system devised by us provides a truly dependable alternative to monitoring traffic flow when emergency vehicles pass by.

  10. Rationally Engineering Phototherapy Modules of Eosin-Conjugated Responsive Polymeric Nanocarriers via Intracellular Endocytic pH Gradients.

    Science.gov (United States)

    Liu, Guhuan; Hu, Jinming; Zhang, Guoying; Liu, Shiyong

    2015-07-15

    Spatiotemporal switching of respective phototherapy modes at the cellular level with minimum side effects and high therapeutic efficacy is a major challenge for cancer phototherapy. Herein we demonstrate how to address this issue by employing photosensitizer-conjugated pH-responsive block copolymers in combination with intracellular endocytic pH gradients. At neutral pH corresponding to extracellular and cytosol milieu, the copolymers self-assemble into micelles with prominently quenched fluorescence emission and low (1)O2 generation capability, favoring a highly efficient photothermal module. Under mildly acidic pH associated with endolysosomes, protonation-triggered micelle-to-unimer transition results in recovered emission and enhanced photodynamic (1)O2 efficiency, which synergistically actuates release of encapsulated drugs, endosomal escape, and photochemical internalization processes.

  11. Traffic Sign Detection and Recognition by Block-based Color Segmentation%基于区域颜色分割的交通标志检测和识别

    Institute of Scientific and Technical Information of China (English)

    金旭晖

    2016-01-01

    现在的高端汽车市场上汽车驾驶辅助摄像系统是一大亮点,研究工作致力于提供一个软件算法来实现驾驶辅助系统中实时交通标志检测和识别的功能。算法的每一步设计都尽量不影响其实时性,基于 HSI 颜色空间,使用了新型的基于区域的快速颜色分割,结合分层技术和简单阈值处理,可以快速地按颜色分层得到采样图像中可能含有标志的感兴趣区域;再在此区域结合数学形态学的膨胀运算以便识别区域形状。最终基于最后输出的感兴趣区域掩模原图像的亮度分量,用边缘方法提取边缘方向的统计特征,进行基于内容的图像检索(CBIR)。初步实验结果,对感兴趣区域的检测准确率在90%以上。并且算法可以扩展到大部分目前标志数据图库中涉及的绝大部分标志使用形状。%In the high-end car market driving assistance camera system is a major attraction now;the research work is dedicated to providing a software algorithm to achieve real-time detection and recognition of traffic signs in driving assistant systems.Every step of the algorithm is designed to minimize the impact to its real-time nature.Based on HSI color space when it uses a new type method of fast block-based color segmentation and combines it with layering technology and simple threshold processing,it is possible to locate quickly as per to colored layers the interested regions in a sampling image which might contain the traffic signs.When dilation operation of mathematical morphology is used in this ROI the region shape may be identified.Based on the intensity vector of the original masked image finally output from the ROI,the statistical features at the edge directions are extracted for content-based image retrieval (CBIR).According to preliminary experimental results,the detection accuracy of the ROI is more than 90%.This algorithm can be extended to most of sign shapes involved in the

  12. Endocytic turnover of Rab8 controls cell polarization

    Science.gov (United States)

    Vidal-Quadras, Maite; Holst, Mikkel R.; Larsson, Elin; Hachimi, Mariam; Yau, Wai-Lok; Peränen, Johan; Martín-Belmonte, Fernando

    2017-01-01

    ABSTRACT Adaptation of cell shape and polarization through the formation and retraction of cellular protrusions requires balancing of endocytosis and exocytosis combined with fine-tuning of the local activity of small GTPases like Rab8. Here, we show that endocytic turnover of the plasma membrane at protrusions is directly coupled to surface removal and inactivation of Rab8. Removal is induced by reduced membrane tension and mediated by the GTPase regulator associated with focal adhesion kinase-1 (GRAF1, also known as ARHGAP26), a regulator of clathrin-independent endocytosis. GRAF1-depleted cells were deficient in multi-directional spreading and displayed elevated levels of GTP-loaded Rab8, which was accumulated at the tips of static protrusions. Furthermore, GRAF1 depletion impaired lumen formation and spindle orientation in a 3D cell culture system, indicating that GRAF1 activity regulates polarity establishment. Our data suggest that GRAF1-mediated removal of Rab8 from the cell surface restricts its activity during protrusion formation, thereby facilitating dynamic adjustment of the polarity axis. PMID:28137756

  13. Differential requirements in endocytic trafficking for penetration of dengue virus.

    Directory of Open Access Journals (Sweden)

    Eliana G Acosta

    Full Text Available The entry of DENV into the host cell appears to be a very complex process which has been started to be studied in detail. In this report, the route of functional intracellular trafficking after endocytic uptake of dengue virus serotype 1 (DENV-1 strain HW, DENV-2 strain NGC and DENV-2 strain 16681 into Vero cells was studied by using a susceptibility to ammonium chloride assay, dominant negative mutants of several members of the family of cellular Rab GTPases that participate in regulation of transport through endosome vesicles and immunofluorescence colocalization. Together, the results presented demonstrate that in spite of the different internalization route among viral serotypes in Vero cells and regardless of the viral strain, DENV particles are first transported to early endosomes in a Rab5-dependent manner. Then a Rab7-dependent pathway guides DENV-2 16681 to late endosomes, whereas a yet unknown sorting event controls the transport of DENV-2 NGC, and most probably DENV-1 HW, to the perinuclear recycling compartments where fusion membrane would take place releasing nucleocapsid into the cytoplasm. Besides the demonstration of a different intracellular trafficking for two DENV-2 strains that shared the initial clathrin-independent internalization route, these studies proved for the first time the involvement of the slow recycling pathway for DENV-2 productive infection.

  14. REGULATION OF Fc RECEPTOR ENDOCYTIC TRAFFICKING BY UBIQUITINATION

    Directory of Open Access Journals (Sweden)

    Rosa eMolfetta

    2014-09-01

    Full Text Available Most immune cells, particularly phagocytes, express various receptors for the Fc-portion of the different immunoglobulin isotypes (Fc receptors, FcRs. By binding to the antibody, they provide a link between the adaptive immune system and the powerful effector functions triggered by innate immune cells such as mast cells, neutrophils, macrophages, and NK cells. Upon ligation of the immune complexes, the downstream signalling pathways initiated by the different receptors are quite similar for different FcR classes leading to the secretion of preformed and de novo synthesized pro-inflammatory mediators. FcR engagement also promotes negative signals through the combined action of several molecules that limit the extent and duration of positive signalling. To this regard, ligand-induced ubiquitination of Fc receptors for IgE (FcεR and IgG (FcγR has become recognized as a key modification that generates signals for the internalization and/or delivery of engaged receptor complexes to lysosomes or cytoplasmic proteasomes for degradation, providing negative-feedback regulation of Fc receptor activity.In this review, we discuss recent advances in our understanding of the molecular mechanisms that ensure the clearance of engaged Fcε and Fcγ receptor complexes from the cell surface with an emphasis given to the cooperation between the ubiquitin pathway and endosomal adaptors including the endosomal sorting complex required for transport (ESCRT in controlling receptor internalization and sorting along the endocytic compartments.

  15. T47D Cells Expressing Myeloperoxidase Are Able to Process, Traffic and Store the Mature Protein in Lysosomes: Studies in T47D Cells Reveal a Role for Cys319 in MPO Biosynthesis that Precedes Its Known Role in Inter-Molecular Disulfide Bond Formation.

    Science.gov (United States)

    Laura, Richard P; Dong, David; Reynolds, Wanda F; Maki, Richard A

    2016-01-01

    Among the human heme-peroxidase family, myeloperoxidase (MPO) has a unique disulfide-linked oligomeric structure resulting from multi-step processing of the pro-protein monomer (proMPO) after it exits the endoplasmic reticulum (ER). Related family members undergo some, but not all, of the processing steps involved with formation of mature MPO. Lactoperoxidase has its pro-domain proteolytically removed and is a monomer in its mature form. Eosinophil peroxidase undergoes proteolytic removal of its pro-domain followed by proteolytic separation into heavy and light chains and is a heterodimer. However, only MPO undergoes both these proteolytic modifications and then is further oligomerized into a heterotetramer by a single inter-molecular disulfide bond. The details of how and where the post-ER processing steps of MPO occur are incompletely understood. We report here that T47D breast cancer cells stably transfected with an MPO expression plasmid are able to efficiently replicate all of the processing steps that lead to formation of the mature MPO heterotetramer. MPO also traffics to the lysosome granules of T47D cells where it accumulates, allowing in-depth immunofluorescent microscopy studies of MPO trafficking and storage for the first time. Using this novel cell model we show that formation of MPO's single inter-molecular disulfide bond can occur normally in the absence of the proteolytic events that lead to separation of the MPO heavy and light chains. We further demonstrate that Cys319, which forms MPO's unique inter-molecular disulfide bond, is important for events that precede this step. Mutation of this residue alters the glycosylation and catalytic activity of MPO and blocks its entry into the endocytic pathway where proteolytic processing and disulfide bonding occur. Finally, using the endocytic trafficking of lysosomal hydrolases as a guide, we investigate the role of candidate receptors in the endocytic trafficking of MPO.

  16. T47D Cells Expressing Myeloperoxidase Are Able to Process, Traffic and Store the Mature Protein in Lysosomes: Studies in T47D Cells Reveal a Role for Cys319 in MPO Biosynthesis that Precedes Its Known Role in Inter-Molecular Disulfide Bond Formation.

    Directory of Open Access Journals (Sweden)

    Richard P Laura

    Full Text Available Among the human heme-peroxidase family, myeloperoxidase (MPO has a unique disulfide-linked oligomeric structure resulting from multi-step processing of the pro-protein monomer (proMPO after it exits the endoplasmic reticulum (ER. Related family members undergo some, but not all, of the processing steps involved with formation of mature MPO. Lactoperoxidase has its pro-domain proteolytically removed and is a monomer in its mature form. Eosinophil peroxidase undergoes proteolytic removal of its pro-domain followed by proteolytic separation into heavy and light chains and is a heterodimer. However, only MPO undergoes both these proteolytic modifications and then is further oligomerized into a heterotetramer by a single inter-molecular disulfide bond. The details of how and where the post-ER processing steps of MPO occur are incompletely understood. We report here that T47D breast cancer cells stably transfected with an MPO expression plasmid are able to efficiently replicate all of the processing steps that lead to formation of the mature MPO heterotetramer. MPO also traffics to the lysosome granules of T47D cells where it accumulates, allowing in-depth immunofluorescent microscopy studies of MPO trafficking and storage for the first time. Using this novel cell model we show that formation of MPO's single inter-molecular disulfide bond can occur normally in the absence of the proteolytic events that lead to separation of the MPO heavy and light chains. We further demonstrate that Cys319, which forms MPO's unique inter-molecular disulfide bond, is important for events that precede this step. Mutation of this residue alters the glycosylation and catalytic activity of MPO and blocks its entry into the endocytic pathway where proteolytic processing and disulfide bonding occur. Finally, using the endocytic trafficking of lysosomal hydrolases as a guide, we investigate the role of candidate receptors in the endocytic trafficking of MPO.

  17. T47D Cells Expressing Myeloperoxidase Are Able to Process, Traffic and Store the Mature Protein in Lysosomes: Studies in T47D Cells Reveal a Role for Cys319 in MPO Biosynthesis that Precedes Its Known Role in Inter-Molecular Disulfide Bond Formation

    Science.gov (United States)

    Laura, Richard P.; Dong, David; Reynolds, Wanda F.; Maki, Richard A.

    2016-01-01

    Among the human heme-peroxidase family, myeloperoxidase (MPO) has a unique disulfide-linked oligomeric structure resulting from multi-step processing of the pro-protein monomer (proMPO) after it exits the endoplasmic reticulum (ER). Related family members undergo some, but not all, of the processing steps involved with formation of mature MPO. Lactoperoxidase has its pro-domain proteolytically removed and is a monomer in its mature form. Eosinophil peroxidase undergoes proteolytic removal of its pro-domain followed by proteolytic separation into heavy and light chains and is a heterodimer. However, only MPO undergoes both these proteolytic modifications and then is further oligomerized into a heterotetramer by a single inter-molecular disulfide bond. The details of how and where the post-ER processing steps of MPO occur are incompletely understood. We report here that T47D breast cancer cells stably transfected with an MPO expression plasmid are able to efficiently replicate all of the processing steps that lead to formation of the mature MPO heterotetramer. MPO also traffics to the lysosome granules of T47D cells where it accumulates, allowing in-depth immunofluorescent microscopy studies of MPO trafficking and storage for the first time. Using this novel cell model we show that formation of MPO’s single inter-molecular disulfide bond can occur normally in the absence of the proteolytic events that lead to separation of the MPO heavy and light chains. We further demonstrate that Cys319, which forms MPO’s unique inter-molecular disulfide bond, is important for events that precede this step. Mutation of this residue alters the glycosylation and catalytic activity of MPO and blocks its entry into the endocytic pathway where proteolytic processing and disulfide bonding occur. Finally, using the endocytic trafficking of lysosomal hydrolases as a guide, we investigate the role of candidate receptors in the endocytic trafficking of MPO. PMID:26890638

  18. The Viral G Protein-Coupled Receptor ORF74 Hijacks β-Arrestins for Endocytic Trafficking in Response to Human Chemokines.

    Science.gov (United States)

    de Munnik, Sabrina M; Kooistra, Albert J; van Offenbeek, Jody; Nijmeijer, Saskia; de Graaf, Chris; Smit, Martine J; Leurs, Rob; Vischer, Henry F

    2015-01-01

    Kaposi's sarcoma-associated herpesvirus-infected cells express the virally encoded G protein-coupled receptor ORF74. Although ORF74 is constitutively active, it binds human CXC chemokines that modulate this basal activity. ORF74-induced signaling has been demonstrated to underlie the development of the angioproliferative tumor Kaposi's sarcoma. Whereas G protein-dependent signaling of ORF74 has been the subject of several studies, the interaction of this viral GPCR with β-arrestins has hitherto not been investigated. Bioluminescence resonance energy transfer experiments demonstrate that ORF74 recruits β-arrestins and subsequently internalizes in response to human CXCL1 and CXCL8, but not CXCL10. Internalized ORF74 traffics via early endosomes to recycling and late endosomes. Site-directed mutagenesis and homology modeling identified four serine and threonine residues at the distal end of the intracellular carboxyl-terminal of ORF74 that are required for β-arrestin recruitment and subsequent endocytic trafficking. Hijacking of the human endocytic trafficking machinery is a previously unrecognized action of ORF74.

  19. Identification and characterization of an unusual class I myosin involved in vesicle traffic in Trypanosoma brucei.

    Directory of Open Access Journals (Sweden)

    Diana Spitznagel

    Full Text Available Myosins are a multimember family of motor proteins with diverse functions in eukaryotic cells. African trypanosomes possess only two candidate myosins and thus represent a useful system for functional analysis of these motors. One of these candidates is an unusual class I myosin (TbMyo1 that is expressed at similar levels but organized differently during the life cycle of Trypanosoma brucei. This myosin localizes to the polarized endocytic pathway in bloodstream forms of the parasite. This organization is actin dependent. Knock down of TbMyo1 results in a significant reduction in endocytic activity, a cessation in cell division and eventually cell death. A striking morphological feature in these cells is an enlargement of the flagellar pocket, which is consistent with an imbalance in traffic to and from the surface. In contrast TbMyo1 is distributed throughout procyclic forms of the tsetse vector and a loss of approximately 90% of the protein has no obvious effects on growth or morphology. These results reveal a life cycle stage specific requirement for this myosin in essential endocytic traffic and represent the first description of the involvement of a motor protein in vesicle traffic in these parasites.

  20. Identification and characterization of an unusual class I myosin involved in vesicle traffic in Trypanosoma brucei.

    Science.gov (United States)

    Spitznagel, Diana; O'Rourke, John F; Leddy, Neal; Hanrahan, Orla; Nolan, Derek P

    2010-01-01

    Myosins are a multimember family of motor proteins with diverse functions in eukaryotic cells. African trypanosomes possess only two candidate myosins and thus represent a useful system for functional analysis of these motors. One of these candidates is an unusual class I myosin (TbMyo1) that is expressed at similar levels but organized differently during the life cycle of Trypanosoma brucei. This myosin localizes to the polarized endocytic pathway in bloodstream forms of the parasite. This organization is actin dependent. Knock down of TbMyo1 results in a significant reduction in endocytic activity, a cessation in cell division and eventually cell death. A striking morphological feature in these cells is an enlargement of the flagellar pocket, which is consistent with an imbalance in traffic to and from the surface. In contrast TbMyo1 is distributed throughout procyclic forms of the tsetse vector and a loss of approximately 90% of the protein has no obvious effects on growth or morphology. These results reveal a life cycle stage specific requirement for this myosin in essential endocytic traffic and represent the first description of the involvement of a motor protein in vesicle traffic in these parasites.

  1. Ubiquitin-Related Roles of β-Arrestins in Endocytic Trafficking and Signal Transduction.

    Science.gov (United States)

    Jean-Charles, Pierre-Yves; Rajiv, Vishwaesh; Shenoy, Sudha K

    2016-10-01

    The non-visual arrestins, β-arrestin1, and β-arrestin2 were originally identified as proteins that bind to seven-transmembrane receptors (7TMRs, also called G protein-coupled receptors, GPCRs) and block heterotrimeric G protein activation, thus leading to desensitization of transmembrane signaling. However, as subsequent discoveries have continually demonstrated, their functionality is not constrained to desensitization. They are now recognized for their critical roles in mediating intracellular trafficking of 7TMRs, growth factor receptors, ion transporters, ion channels, nuclear receptors, and non-receptor proteins. Additionally, they function as crucial mediators of ubiquitination of 7TMRs as well as other receptors and non-receptor proteins. Recently, emerging studies suggest that a class of proteins with predicted structural features of β-arrestins regulate substrate ubiquitination in yeast and higher mammals, lending support to the idea that the adaptor role of β-arrestins in protein ubiquitination is evolutionarily conserved. β-arrestins also function as scaffolds for kinases and transduce signals from 7TMRs through pathways that do not require G protein activation. Remarkably, the endocytic and scaffolding functions of β-arrestin are intertwined with its ubiquitination status; the dynamic and site specific ubiquitination on β-arrestin plays a critical role in stabilizing β-arrestin-7TMR association and the formation of signalosomes. This review summarizes the current findings on ubiquitin-dependent regulation of 7TMRs as well as β-arrestins and the potential role of reversible ubiquitination as a "biological switch" in signal transduction. J. Cell. Physiol. 231: 2071-2080, 2016. © 2016 Wiley Periodicals, Inc.

  2. Probiotics promote endocytic allergen degradation in gut epithelial cells

    Energy Technology Data Exchange (ETDEWEB)

    Song, Chun-Hua [Department of Epidemiology and Biostatistics, College of Public Health, Zhengzhou University, Zhengzhou (China); Liu, Zhi-Qiang [Department of Gastroenterology, The Second Hospital, Zhengzhou University, Zhengzhou (China); Department of Pathology and Molecular Medicine, McMaster University, Hamilton, ON (Canada); Huang, Shelly [Department of Pathology and Molecular Medicine, McMaster University, Hamilton, ON (Canada); Zheng, Peng-Yuan, E-mail: medp7123@126.com [Department of Gastroenterology, The Second Hospital, Zhengzhou University, Zhengzhou (China); Yang, Ping-Chang, E-mail: yangp@mcmaster.ca [Department of Pathology and Molecular Medicine, McMaster University, Hamilton, ON (Canada)

    2012-09-14

    Highlights: Black-Right-Pointing-Pointer Knockdown of A20 compromised the epithelial barrier function. Black-Right-Pointing-Pointer The fusion of endosome/lysosome was disturbed in the A20-deficient HT-29 cells. Black-Right-Pointing-Pointer Antigens transported across A20-deficient HT-29 monolayers conserved antigenicity. Black-Right-Pointing-Pointer Probiotic proteins increased the expression of A20 in HT-29 cells. -- Abstract: Background and aims: Epithelial barrier dysfunction plays a critical role in the pathogenesis of allergic diseases; the mechanism is to be further understood. The ubiquitin E3 ligase A20 (A20) plays a role in the endocytic protein degradation in the cells. This study aims to elucidate the role of A20 in the maintenance of gut epithelial barrier function. Methods: Gut epithelial cell line, HT-29 cell, was cultured into monolayers to evaluate the barrier function in transwells. RNA interference was employed to knock down the A20 gene in HT-29 cells to test the role of A20 in the maintenance of epithelial barrier function. Probiotic derived proteins were extracted from the culture supernatants using to enhance the expression of A20 in HT-29 cells. Results: The results showed that the knockdown of A20 compromised the epithelial barrier function in HT-29 monolayers, mainly increased the intracellular permeability. The fusion of endosome/lysosome was disturbed in the A20-deficient HT-29 cells. Allergens collected from the transwell basal chambers of A20-deficient HT-29 monolayers still conserved functional antigenicity. Treating with probiotic derived proteins increased the expression of A20 in HT-29 cells and promote the barrier function. Conclusion: A20 plays an important role in the maintenance of epithelial barrier function as shown by HT-29 monolayer. Probiotic derived protein increases the expression of A20 and promote the HT-29 monolayer barrier function.

  3. Dimerization drives EGFR endocytosis through two sets of compatible endocytic codes.

    Science.gov (United States)

    Wang, Qian; Chen, Xinmei; Wang, Zhixiang

    2015-03-01

    We have shown previously that epidermal growth factor (EGF) receptor (EGFR) endocytosis is controlled by EGFR dimerization. However, it is not clear how the dimerization drives receptor internalization. We propose that EGFR endocytosis is driven by dimerization, bringing two sets of endocytic codes, one contained in each receptor monomer, in close proximity. Here, we tested this hypothesis by generating specific homo- or hetero-dimers of various receptors and their mutants. We show that ErbB2 and ErbB3 homodimers are endocytosis deficient owing to the lack of endocytic codes. Interestingly, EGFR-ErbB2 or EGFR-ErbB3 heterodimers are also endocytosis deficient. Moreover, the heterodimer of EGFR and the endocytosis-deficient mutant EGFRΔ1005-1017 is also impaired in endocytosis. These results indicate that two sets of endocytic codes are required for receptor endocytosis. We found that an EGFR-PDGFRβ heterodimer is endocytosis deficient, although both EGFR and PDGFRβ homodimers are endocytosis-competent, indicating that two compatible sets of endocytic codes are required. Finally, we found that to mediate the endocytosis of the receptor dimer, the two sets of compatible endocytic codes, one contained in each receptor molecule, have to be spatially coordinated.

  4. Endocytic sorting and recycling require membrane phosphatidylserine asymmetry maintained by TAT-1/CHAT-1.

    Science.gov (United States)

    Chen, Baohui; Jiang, Yue; Zeng, Sheng; Yan, Jiacong; Li, Xin; Zhang, Yan; Zou, Wei; Wang, Xiaochen

    2010-12-09

    Endocytic sorting is achieved through the formation of morphologically and functionally distinct sub-domains within early endosomes. Cargoes destined for recycling are sorted to and transported through newly-formed tubular membranes, but the processes that regulate membrane tubulation are poorly understood. Here, we identified a novel Caenorhabditis elegans Cdc50 family protein, CHAT-1, which acts as the chaperone of the TAT-1 P4-ATPase to regulate membrane phosphatidylserine (PS) asymmetry and endocytic transport. In chat-1 and tat-1 mutants, the endocytic sorting process is disrupted, leading to defects in both cargo recycling and degradation. TAT-1 and CHAT-1 colocalize to the tubular domain of the early endosome, the tubular endocytic recycling compartment (ERC), and the recycling endosome where PS is enriched on the cytosolic surface. Loss of tat-1 and chat-1 function disrupts membrane PS asymmetry and abrogates the tubular membrane structure. Our data suggest that CHAT-1 and TAT-1 maintain membrane phosphatidylserine asymmetry, thus promoting membrane tubulation and regulating endocytic sorting and recycling.

  5. Quantum dot-loaded monofunctionalized DNA icosahedra for single-particle tracking of endocytic pathways

    Science.gov (United States)

    Bhatia, Dhiraj; Arumugam, Senthil; Nasilowski, Michel; Joshi, Himanshu; Wunder, Christian; Chambon, Valérie; Prakash, Ved; Grazon, Chloé; Nadal, Brice; Maiti, Prabal K.; Johannes, Ludger; Dubertret, Benoit; Krishnan, Yamuna

    2016-12-01

    Functionalization of quantum dots (QDs) with a single biomolecular tag using traditional approaches in bulk solution has met with limited success. DNA polyhedra consist of an internal void bounded by a well-defined three-dimensional structured surface. The void can house cargo and the surface can be functionalized with stoichiometric and spatial precision. Here, we show that monofunctionalized QDs can be realized by encapsulating QDs inside DNA icosahedra and functionalizing the DNA shell with an endocytic ligand. We deployed the DNA-encapsulated QDs for real-time imaging of three different endocytic ligands—folic acid, galectin-3 (Gal3) and the Shiga toxin B-subunit (STxB). Single-particle tracking of Gal3- or STxB-functionalized QD-loaded DNA icosahedra allows us to monitor compartmental dynamics along endocytic pathways. These DNA-encapsulated QDs, which bear a unique stoichiometry of endocytic ligands, represent a new class of molecular probes for quantitative imaging of endocytic receptor dynamics.

  6. Endocytic downregulation of ErbB receptors: mechanisms and relevance in cancer

    DEFF Research Database (Denmark)

    Roepstorff, Kirstine; Grøvdal, Lene; Grandal, Michael;

    2008-01-01

    ErbB receptors (EGFR (ErbB1), ErbB2, ErbB3, and ErbB4) are important regulators of normal growth and differentiation, and they are involved in the pathogenesis of cancer. Following ligand binding and receptor activation, EGFR is endocytosed and transported to lysosomes where the receptor...... is degraded. This downregulation of EGFR is a complex and tightly regulated process. The functions of ErbB2, ErbB3, and ErbB4 are also regulated by endocytosis to some extent, although the current knowledge of these processes is sparse. Impaired endocytic downregulation of signaling receptors is frequently...... associated with cancer, since it can lead to increased and uncontrolled receptor signaling. In this review we describe the current knowledge of ErbB receptor endocytic downregulation. In addition, we outline how ErbB receptors can escape endocytic downregulation in cancer, and we discuss how targeted anti...

  7. Functional links between Aβ toxicity, endocytic trafficking, and Alzheimer's disease risk factors in yeast.

    Science.gov (United States)

    Treusch, Sebastian; Hamamichi, Shusei; Goodman, Jessica L; Matlack, Kent E S; Chung, Chee Yeun; Baru, Valeriya; Shulman, Joshua M; Parrado, Antonio; Bevis, Brooke J; Valastyan, Julie S; Han, Haesun; Lindhagen-Persson, Malin; Reiman, Eric M; Evans, Denis A; Bennett, David A; Olofsson, Anders; DeJager, Philip L; Tanzi, Rudolph E; Caldwell, Kim A; Caldwell, Guy A; Lindquist, Susan

    2011-12-01

    Aβ (beta-amyloid peptide) is an important contributor to Alzheimer's disease (AD). We modeled Aβ toxicity in yeast by directing the peptide to the secretory pathway. A genome-wide screen for toxicity modifiers identified the yeast homolog of phosphatidylinositol binding clathrin assembly protein (PICALM) and other endocytic factors connected to AD whose relationship to Aβ was previously unknown. The factors identified in yeast modified Aβ toxicity in glutamatergic neurons of Caenorhabditis elegans and in primary rat cortical neurons. In yeast, Aβ impaired the endocytic trafficking of a plasma membrane receptor, which was ameliorated by endocytic pathway factors identified in the yeast screen. Thus, links between Aβ, endocytosis, and human AD risk factors can be ascertained with yeast as a model system.

  8. Endocytic Pathways Involved in Filovirus Entry: Advances, Implications and Future Directions

    Directory of Open Access Journals (Sweden)

    Suchita Bhattacharyya

    2012-12-01

    Full Text Available Detailed knowledge of the host-virus interactions that accompany filovirus entry into cells is expected to identify determinants of viral virulence and host range, and to yield targets for the development of antiviral therapeutics. While it is generally agreed that filovirus entry into the host cytoplasm requires viral internalization into acidic endosomal compartments and proteolytic cleavage of the envelope glycoprotein by endo/lysosomal cysteine proteases, our understanding of the specific endocytic pathways co-opted by filoviruses remains limited. This review addresses the current knowledge on cellular endocytic pathways implicated in filovirus entry, highlights the consensus as well as controversies, and discusses important remaining questions.

  9. Inhibition of endocytic vesicle fusion by Plk1-mediated phosphorylation of vimentin during mitosis.

    Science.gov (United States)

    Ikawa, Keisuke; Satou, Ayaka; Fukuhara, Mitsuko; Matsumura, Shigeru; Sugiyama, Naoyuki; Goto, Hidemasa; Fukuda, Mitsunori; Inagaki, Masaki; Ishihama, Yasushi; Toyoshima, Fumiko

    2014-01-01

    Endocytic vesicle fusion is inhibited during mitosis, but the molecular pathways that mediate the inhibition remain unclear. Here we uncovered an essential role of Polo-like kinase 1 (Plk1) in this mechanism. Phosphoproteomic analysis revealed that Plk1 phosphorylates the intermediate filament protein vimentin on Ser459, which is dispensable for its filament formation but is necessary for the inhibition of endocytic vesicle fusion in mitosis. Furthermore, this mechanism is required for integrin trafficking toward the cleavage furrow during cytokinesis. Our results thus identify a novel mechanism for fusion inhibition in mitosis and implicate its role in vesicle trafficking after anaphase onset.

  10. Jamitons: Phantom Traffic Jams

    Science.gov (United States)

    Kowszun, Jorj

    2013-01-01

    Traffic on motorways can slow down for no apparent reason. Sudden changes in speed by one or two drivers can create a chain reaction that causes a traffic jam for the vehicles that are following. This kind of phantom traffic jam is called a "jamiton" and the article discusses some of the ways in which traffic engineers produce…

  11. TRANSIMS traffic flow characteristics

    CERN Document Server

    Nagel, K; Pieck, M; Donnelly, R; Barrett, C L; Nagel, Kai; Stretz, Paula; Pieck, Martin; Donnelly, Rick; Barrett, Christopher L.

    1997-01-01

    Knowledge of fundamental traffic flow characteristics of traffic simulation models is an essential requirement when using these models for the planning, design, and operation of transportation systems. In this paper we discuss the following: a description of how features relevant to traffic flow are currently under implementation in the TRANSIMS microsimulation, a proposition for standardized traffic flow tests for traffic simulation models, and the results of these tests for two different versions of the TRANSIMS microsimulation.

  12. Car Delay Model near Bus Stops with Mixed Traffic Flow

    OpenAIRE

    Yang Xiaobao; Huan Mei; Gao Ziyou

    2013-01-01

    This paper proposes a model for estimating car delays at bus stops under mixed traffic using probability theory and queuing theory. The roadway is divided to serve motorized and nonmotorized traffic streams. Bus stops are located on the nonmotorized lanes. When buses dwell at the stop, they block the bicycles. Thus, two conflict points between car stream and other traffic stream are identified. The first conflict point occurs as bicycles merge to the motorized lane to avoid waiting behind the...

  13. The LXR-IDOL axis defines a clathrin-, caveolae-, and dynamin-independent endocytic route for LDLR internalization and lysosomal degradation[S

    Science.gov (United States)

    Sorrentino, Vincenzo; Nelson, Jessica K.; Maspero, Elena; Marques, André R. A.; Scheer, Lilith; Polo, Simona; Zelcer, Noam

    2013-01-01

    Low density lipoprotein (LDL) cholesterol is taken up into cells via clathrin-mediated endocytosis of the LDL receptor (LDLR). Following dissociation of the LDLR-LDL complex, LDL is directed to lysosomes whereas the LDLR recycles to the plasma membrane. Activation of the sterol-sensing nuclear receptors liver X receptors (LXRs) enhances degradation of the LDLR. This depends on the LXR target gene inducible degrader of the LDLR (IDOL), an E3-ubiquitin ligase that promotes ubiquitylation and lysosomal degradation of the LDLR. How ubiquitylation of the LDLR by IDOL controls its endocytic trafficking is currently unknown. Using genetic- and pharmacological-based approaches coupled to functional assessment of LDL uptake, we show that the LXR-IDOL axis targets a LDLR pool present in lipid rafts. IDOL-dependent internalization of the LDLR is independent of clathrin, caveolin, macroautophagy, and dynamin. Rather, it depends on the endocytic protein epsin. Consistent with LDLR ubiquitylation acting as a sorting signal, degradation of the receptor can be blocked by perturbing the endosomal sorting complex required for transport (ESCRT) or by USP8, a deubiquitylase implicated in sorting ubiquitylated cargo to multivesicular bodies. In summary, we provide evidence for the existence of an LXR-IDOL-mediated internalization pathway for the LDLR that is distinct from that used for lipoprotein uptake. PMID:23733886

  14. Vehicle-class Specific Control of Freeway Traffic

    NARCIS (Netherlands)

    Schreiter, T.

    2013-01-01

    The increase of mobility of the past decades has led to substantial congestion on the freeways. Traffic jams emerge both on a daily basis at the same location, as well as during accidents when a part of the freeways is temporarily blocked. In those cases, traffic management centers intervene into tr

  15. 49 CFR 236.828 - System, traffic control.

    Science.gov (United States)

    2010-10-01

    ... 49 Transportation 4 2010-10-01 2010-10-01 false System, traffic control. 236.828 Section 236.828..., MAINTENANCE, AND REPAIR OF SIGNAL AND TRAIN CONTROL SYSTEMS, DEVICES, AND APPLIANCES Definitions § 236.828 System, traffic control. A block signal system under which train movements are authorized by...

  16. Rock blocks

    OpenAIRE

    Turner, W.

    2007-01-01

    Consider representation theory associated to symmetric groups, or to Hecke algebras in type A, or to q-Schur algebras, or to finite general linear groups in non-describing characteristic. Rock blocks are certain combinatorially defined blocks appearing in such a representation theory, first observed by R. Rouquier. Rock blocks are much more symmetric than general blocks, and every block is derived equivalent to a Rock block. Motivated by a theorem of J. Chuang and R. Kessar in the case of sym...

  17. Gliadin peptide P31-43 localises to endocytic vesicles and interferes with their maturation.

    Directory of Open Access Journals (Sweden)

    Maria Vittoria Barone

    Full Text Available BACKGROUND: Celiac Disease (CD is both a frequent disease (1:100 and an interesting model of a disease induced by food. It consists in an immunogenic reaction to wheat gluten and glutenins that has been found to arise in a specific genetic background; however, this reaction is still only partially understood. Activation of innate immunity by gliadin peptides is an important component of the early events of the disease. In particular the so-called "toxic" A-gliadin peptide P31-43 induces several pleiotropic effects including Epidermal Growth Factor Receptor (EGFR-dependent actin remodelling and proliferation in cultured cell lines and in enterocytes from CD patients. These effects are mediated by delayed EGFR degradation and prolonged EGFR activation in endocytic vesicles. In the present study we investigated the effects of gliadin peptides on the trafficking and maturation of endocytic vesicles. METHODS/PRINCIPAL FINDINGS: Both P31-43 and the control P57-68 peptide labelled with fluorochromes were found to enter CaCo-2 cells and interact with the endocytic compartment in pulse and chase, time-lapse, experiments. P31-43 was localised to vesicles carrying early endocytic markers at time points when P57-68-carrying vesicles mature into late endosomes. In time-lapse experiments the trafficking of P31-43-labelled vesicles was delayed, regardless of the cargo they were carrying. Furthermore in celiac enterocytes, from cultured duodenal biopsies, P31-43 trafficking is delayed in early endocytic vesicles. A sequence similarity search revealed that P31-43 is strikingly similar to Hrs, a key molecule regulating endocytic maturation. A-gliadin peptide P31-43 interfered with Hrs correct localisation to early endosomes as revealed by western blot and immunofluorescence microscopy. CONCLUSIONS: P31-43 and P57-68 enter cells by endocytosis. Only P31-43 localises at the endocytic membranes and delays vesicle trafficking by interfering with Hrs

  18. Negative regulation of the endocytic adaptor disabled-2 (Dab2) in mitosis.

    Science.gov (United States)

    Chetrit, David; Barzilay, Lior; Horn, Galit; Bielik, Tom; Smorodinsky, Nechama I; Ehrlich, Marcelo

    2011-02-18

    Mitotic cells undergo extensive changes in shape and size through the altered regulation and function of their membrane trafficking machinery. Disabled 2 (Dab2), a multidomain cargo-specific endocytic adaptor and a mediator of signal transduction, is a potential integrator of trafficking and signaling. Dab2 binds effectors of signaling and trafficking that localize to different intracellular compartments. Thus, differential localization is a putative regulatory mechanism of Dab2 function. Furthermore, Dab2 is phosphorylated in mitosis and is thus regulated in the cell cycle. However, a detailed description of the intracellular localization of Dab2 in the different phases of mitosis and an understanding of the functional consequences of its phosphorylation are lacking. Here, we show that Dab2 is progressively displaced from the membrane in mitosis. This phenomenon is paralleled by a loss of co-localization with clathrin. Both phenomena culminate in metaphase/anaphase and undergo partial recovery in cytokinesis. Treatment with 2-methoxyestradiol, which arrests cells at the spindle assembly checkpoint, induces the same effects observed in metaphase cells. Moreover, 2-methoxyestradiol also induced Dab2 phosphorylation and reduced Dab2/clathrin interactions, endocytic vesicle motility, clathrin exchange dynamics, and the internalization of a receptor endowed with an NPXY endocytic signal. Serine/threonine to alanine mutations, of residues localized to the central region of Dab2, attenuated its phosphorylation, reduced its membrane displacement, and maintained its endocytic abilities in mitosis. We propose that the negative regulation of Dab2 is part of an accommodation of the cell to the altered physicochemical conditions prevalent in mitosis, aimed at allowing endocytic activity throughout the cell cycle.

  19. Trip-timing decisions with traffic incidents

    DEFF Research Database (Denmark)

    Fosgerau, Mogens; Lindsey, Robin

    2013-01-01

    This paper analyzes traffic bottleneck congestion when drivers randomly cause incidents that temporarily block the bottleneck. Drivers have general scheduling preferences for time spent at home and at work. They independently choose morning departure times from home to maximize expected utility...

  20. Mutational analysis of the yeast TRAPP subunit Trs20p identifies roles in endocytic recycling and sporulation.

    Directory of Open Access Journals (Sweden)

    Hichem Mahfouz

    Full Text Available Trs20p is a subunit of the evolutionarily conserved TRAPP (TRAnsport Protein Particle complex that mediates various aspects of membrane trafficking. Three TRAPP complexes have been identified in yeast with roles in ER-to-Golgi trafficking, post-Golgi and endosomal-to-Golgi transport and in autophagy. The role of Trs20p, which is essential for viability and a component of all three complexes, and how it might function within each TRAPP complex, has not been clarified to date. To begin to address the role of Trs20p we generated different mutants by random mutagenesis but, surprisingly, no defects were observed in diverse anterograde transport pathways or general secretion in Trs20 temperature-sensitive mutants. Instead, mutation of Trs20 led to defects in endocytic recycling and a block in sporulation/meiosis. The phenotypes of different mutants appear to be separable suggesting that the mutations affect the function of Trs20 in different TRAPP complexes.

  1. Integrin-beta3 clusters recruit clathrin-mediated endocytic machinery in the absence of traction force

    Science.gov (United States)

    Yu, Cheng-han; Rafiq, Nisha Bte Mohd; Cao, Fakun; Zhou, Yuhuan; Krishnasamy, Anitha; Biswas, Kabir Hassan; Ravasio, Andrea; Chen, Zhongwen; Wang, Yu-Hsiu; Kawauchi, Keiko; Jones, Gareth E.; Sheetz, Michael P.

    2015-01-01

    The turnover of integrin receptors is critical for cell migration and adhesion dynamics. Here we find that force development at integrins regulates adaptor protein recruitment and endocytosis. Using mobile RGD (Arg-Gly-Asp) ligands on supported lipid membranes (RGD membranes) and rigid RGD ligands on glass (RGD-glass), we find that matrix force-dependent integrin signals block endocytosis. Dab2, an adaptor protein of clathrin-mediated endocytosis, is not recruited to activated integrin-beta3 clusters on RGD-glass; however, it is recruited to integrin-mediated adhesions on RGD membranes. Further, when force generation is inhibited on RGD-glass, Dab2 binds to integrin-beta3 clusters. Dab2 binding to integrin-beta3 excludes other adhesion-related adaptor proteins, such as talin. The clathrin-mediated endocytic machinery combines with Dab2 to facilitate the endocytosis of RGD-integrin-beta3 clusters. From these observations, we propose that loss of traction force on ligand-bound integrin-beta3 causes recruitment of Dab2/clathrin, resulting in endocytosis of integrins. PMID:26507506

  2. Subjective safety in traffic.

    NARCIS (Netherlands)

    2012-01-01

    The term ‘subjective safety in traffic’ refers to people feeling unsafe in traffic or, more generally, to anxiety regarding being unsafe in traffic for oneself and/or others. Subjective safety in traffic can lead to road users limiting their mobility and social activities, which is one of the reason

  3. Traffic Sign Recognition System based on Cambridge Correlator Image Comparator

    Directory of Open Access Journals (Sweden)

    J. Turan

    2012-06-01

    Full Text Available Paper presents basic information about application of Optical Correlator (OC, specifically Cambridge Correlator, in system to recognize of traffic sign. Traffic Sign Recognition System consists of three main blocks, Preprocessing, Optical Correlator and Traffic Sign Identification. The Region of Interest (ROI is defined and chosen in preprocessing block and then goes to Optical Correlator, where is compared with database of Traffic Sign. Output of Optical Correlation is correlation plane, which consist of highly localized intensities, know as correlation peaks. The intensity of spots provides a measure of similarity and position of spots, how images (traffic signs are relatively aligned in the input scene. Several experiments have been done with proposed system and results and conclusion are discussed.

  4. Dynamic multicast traffic grooming in WDM networks

    Institute of Scientific and Technical Information of China (English)

    CHENG Xiao-jun; GE Ning; FENG Chong-xi

    2006-01-01

    Dynamic multicast traffic grooming in wavelength division multiplexing (WDM) networks was analyzed to minimize networkwide costs and to increase the network resource utilization.A network model was developed for dynamic multicast traffic grooming with resource constraints and an algorithm that can provide quality of service (QoS)was proposed.The QoS is measured by the maximum number of lightpaths passing between the source and the destinations.The blocking probability of the algorithm was assessed in simulations.The results show that a higher QoS requirement results in higher blocking probability,and when the QoS requirement is low,changes in the QoS requirements have only small effects on the blocking probability.

  5. DYNAMIC RESOURCE ALLOCATION SCHEME UNDER TRAFFIC CONDITION IN SATELLITE SYSTEMS

    Institute of Scientific and Technical Information of China (English)

    Yu Jia; Zong Peng

    2012-01-01

    As the traffic distribution in China mainland is far from uniform,a new traffic model in China mainland is presented on the basis of per-capita Gross Domestic Product (GDP) and density of population.Based on this characteristic traffic model,a new Traffic Dependent Dynamic Channel Allocation and Reservation (TDDCAR) technique is proposed,the simulation model is built,and the strategies' performance is evaluated through computer simulation.The simulation results show that,compared to the conventional Fixed Channel Allocation (FCA),TDDCAR estimates the traffic conditions in every spot beam and frequently adjusts the traffic according to current traffic conditions.Ithas achieved a significant improvement in new call blocking probability,handover blocking probability,and fair index,particularly,in heavy traffic conditions.The building of traffic model in China mainland and the analysis of the simulation results has been a key foundation for the study of resource allocation schemes in the future.

  6. Salmonella Disrupts Host Endocytic Trafficking by SopD2-Mediated Inhibition of Rab7

    Directory of Open Access Journals (Sweden)

    Vanessa M. D’Costa

    2015-09-01

    Full Text Available Intracellular bacterial pathogens of a diverse nature share the ability to evade host immunity by impairing trafficking of endocytic cargo to lysosomes for degradation, a process that is poorly understood. Here, we show that the Salmonella enterica type 3 secreted effector SopD2 mediates this process by binding the host regulatory GTPase Rab7 and inhibiting its nucleotide exchange. Consequently, this limits Rab7 interaction with its dynein- and kinesin-binding effectors RILP and FYCO1 and thereby disrupts host-driven regulation of microtubule motors. Our study identifies a bacterial effector capable of directly binding and thereby modulating Rab7 activity and a mechanism of endocytic trafficking disruption that may provide insight into the pathogenesis of other bacteria. Additionally, we provide a powerful tool for the study of Rab7 function, and a potential therapeutic target.

  7. The convergent point of the endocytic and autophagic pathways in leydig cells

    Institute of Scientific and Technical Information of China (English)

    YIJING; XUEMINGTANG

    1999-01-01

    Endocytic tracers and marker enzyme of lysosomes were used in the present study to analyze the processes of autophagocytosis and endocytosis,and the convergent point of these two pathways in Leydig cells.The endocytic and autophagic compartments can be easily identified in Leydig cells,which makes easier to difine the stages of two pathways than was possible before.The evidences indicated that late endosomes (dense MVBs) deliver their endocytosed gold tracers together with lysosomal enzymes to the early autophagosomes and they are the convergent point of the two pathways.During this convergent process,the early autophadosomes transform into late autophagosomes and the late endosomes transform into mature lysosomes.

  8. Adapting for endocytosis: Roles for endocytic sorting adaptors in directing neural development.

    Directory of Open Access Journals (Sweden)

    Chan Choo eYap

    2015-04-01

    Full Text Available Proper cortical development depends on the orchestrated actions of a multitude of guidance receptors and adhesion molecules and their downstream signaling. The levels of these receptors on the surface and their precise locations can greatly affect guidance outcomes. Trafficking of receptors to a particular surface locale and removal by endocytosis thus feed crucially into the final guidance outcomes. In addition, endocytosis of receptors can affect downstream signaling (both quantitatively and qualitatively and regulated endocytosis of guidance receptors is thus an important component of ensuring proper neural development. We will discuss the cell biology of regulated endocytosis and the impact on neural development. We focus our discussion on endocytic accessory proteins (such as numb and disabled and how they regulate endocytosis and subsequent post-endocytic trafficking of their cognate receptors (such as Notch, TrkB, β-APP, VLDLR, and ApoER2.

  9. Adapting for endocytosis: roles for endocytic sorting adaptors in directing neural development.

    Science.gov (United States)

    Yap, Chan Choo; Winckler, Bettina

    2015-01-01

    Proper cortical development depends on the orchestrated actions of a multitude of guidance receptors and adhesion molecules and their downstream signaling. The levels of these receptors on the surface and their precise locations can greatly affect guidance outcomes. Trafficking of receptors to a particular surface locale and removal by endocytosis thus feed crucially into the final guidance outcomes. In addition, endocytosis of receptors can affect downstream signaling (both quantitatively and qualitatively) and regulated endocytosis of guidance receptors is thus an important component of ensuring proper neural development. We will discuss the cell biology of regulated endocytosis and the impact on neural development. We focus our discussion on endocytic accessory proteins (EAPs) (such as numb and disabled) and how they regulate endocytosis and subsequent post-endocytic trafficking of their cognate receptors (such as Notch, TrkB, β-APP, VLDLR, and ApoER2).

  10. Interpretation of the FGF8 morphogen gradient is regulated by endocytic trafficking.

    Science.gov (United States)

    Nowak, Matthias; Machate, Anja; Yu, Shuizi Rachel; Gupta, Mansi; Brand, Michael

    2011-02-01

    Forty years ago, it was proposed that during embryonic development and organogenesis, morphogen gradients provide positional information to the individual cells within a tissue leading to specific fate decisions. Recently, much insight has been gained into how such morphogen gradients are formed and maintained; however, which cellular mechanisms govern their interpretation within target tissues remains debated. Here we used in vivo fluorescence correlation spectroscopy and automated image analysis to assess the role of endocytic sorting dynamics on fibroblast growth factor 8 (Fgf8) morphogen gradient interpretation. By interfering with the function of the ubiquitin ligase Cbl, we found an expanded range of Fgf target gene expression and a delay of Fgf8 lysosomal transport. However, the extracellular Fgf8 morphogen gradient remained unchanged, indicating that the observed signalling changes are due to altered gradient interpretation. We propose that regulation of morphogen signalling activity through endocytic sorting allows fast feedback-induced changes in gradient interpretation during the establishment of complex patterns.

  11. A Marine Traffic Flow Model

    Directory of Open Access Journals (Sweden)

    Tsz Leung Yip

    2013-03-01

    Full Text Available A model is developed for studying marine traffic flow through classical traffic flow theories, which can provide us with a better understanding of the phenomenon of traffic flow of ships. On one hand, marine traffic has its special features and is fundamentally different from highway, air and pedestrian traffic. The existing traffic models cannot be simply extended to marine traffic without addressing marine traffic features. On the other hand, existing literature on marine traffic focuses on one ship or two ships but does not address the issues in marine traffic flow.

  12. A Comparative Study on the Alterations of Endocytic Pathways in Multiple Lysosomal Storage Disorders.

    Science.gov (United States)

    Rappaport, Jeff; Manthe, Rachel L; Solomon, Melani; Garnacho, Carmen; Muro, Silvia

    2016-02-01

    Many cellular activities and pharmaceutical interventions involve endocytosis and delivery to lysosomes for processing. Hence, lysosomal processing defects can cause cell and tissue damage, as in lysosomal storage diseases (LSDs) characterized by lysosomal accumulation of undegraded materials. This storage causes endocytic and trafficking alterations, which exacerbate disease and hinder treatment. However, there have been no systematic studies comparing different endocytic routes in LSDs. Here, we used genetic and pharmacological models of four LSDs (type A Niemann-Pick, type C Niemann-Pick, Fabry, and Gaucher diseases) and evaluated the pinocytic and receptor-mediated activity of the clathrin-, caveolae-, and macropinocytic routes. Bulk pinocytosis was diminished in all diseases, suggesting a generic endocytic alteration linked to lysosomal storage. Fluid-phase (dextran) and ligand (transferrin) uptake via the clathrin route were lower for all LSDs. Fluid-phase and ligand (cholera toxin B) uptake via the caveolar route were both affected but less acutely in Fabry or Gaucher diseases. Epidermal growth factor-induced macropinocytosis was altered in Niemann-Pick cells but not other LSDs. Intracellular trafficking of ligands was also distorted in LSD versus wild-type cells. The extent of these endocytic alterations paralleled the level of cholesterol storage in disease cell lines. Confirming this, pharmacological induction of cholesterol storage in wild-type cells disrupted endocytosis, and model therapeutics restored uptake in proportion to their efficacy in attenuating storage. This suggests a proportional and reversible relationship between endocytosis and lipid (cholesterol) storage. By analogy, the accumulation of biological material in other diseases, or foreign material from drugs or their carriers, may cause similar deficits, warranting further investigation.

  13. Implications of traffic characteristics on interdomain traffic engineering

    OpenAIRE

    Uhlig, Steve

    2004-01-01

    This thesis discusses the implications of the traffic characteristics on interdomain traffic engineering with BGP. We first provide an overview of the interdomain traffic control problem. Then, we present results concerning the characteristics of the interdomain traffic, based on the analysis of real traffic traces gathered from non-transit ASes. We discuss the implications of the topological properties of the traffic on interdomain traffic engineering. Based on this knowledge of the...

  14. The minute virus of mice exploits different endocytic pathways for cellular uptake

    Energy Technology Data Exchange (ETDEWEB)

    Garcin, Pierre O.; Panté, Nelly, E-mail: pante@zoology.ubc.ca

    2015-08-15

    The minute virus of mice, prototype strain (MVMp), is a non-enveloped, single-stranded DNA virus of the family Parvoviridae. Unlike other parvoviruses, the mechanism of cellular uptake of MVMp has not been studied in detail. We analyzed MVMp endocytosis in mouse LA9 fibroblasts and a tumor cell line derived from epithelial–mesenchymal transition through polyomavirus middle T antigen transformation in transgenic mice. By a combination of immunofluorescence and electron microscopy, we found that MVMp endocytosis occurs at the leading edge of migrating cells in proximity to focal adhesion sites. By using drug inhibitors of various endocytic pathways together with immunofluorescence microscopy and flow cytometry analysis, we discovered that MVMp can use a number of endocytic pathways, depending on the host cell type. At least three different mechanisms were identified: clathrin-, caveolin-, and clathrin-independent carrier-mediated endocytosis, with the latter occurring in transformed cells but not in LA9 fibroblasts. - Highlights: • MVMp uptake takes place at the leading edge of migrating cells. • MVMp exploits a variety of endocytic pathways. • MVMp could use clathrin- and caveolin-mediated endocytosis. • MVMp could also use clathrin-independent carriers for cellular uptake.

  15. Population Blocks.

    Science.gov (United States)

    Smith, Martin H.

    1992-01-01

    Describes an educational game called "Population Blocks" that is designed to illustrate the concept of exponential growth of the human population and some potential effects of overpopulation. The game material consists of wooden blocks; 18 blocks are painted green (representing land), 7 are painted blue (representing water); and the remaining…

  16. Costs of traffic injuries

    DEFF Research Database (Denmark)

    Kruse, Marie

    2015-01-01

    assessed using Danish national healthcare registers. Productivity costs were computed using duration analysis (Cox regression models). In a subanalysis, cost per severe traffic injury was computed for the 12 995 individuals that experienced a severe injury. RESULTS: The socioeconomic cost of a traffic......OBJECTIVE: The aim of this study was to analyse the socioeconomic costs of traffic injuries in Denmark, notably the healthcare costs and the productivity costs related to traffic injuries, in a bottom-up, register-based perspective. METHOD: Traffic injury victims were identified using national...... emergency room data and police records. Victims were matched with five controls per case by means of propensity score, nearest-neighbour matching. In the cohort, consisting of the 52 526 individuals that experienced a traffic injury in 2000 and 262 630 matched controls, attributable healthcare costs were...

  17. ΔF508 CFTR surface stability is regulated by DAB2 and CHIP-mediated ubiquitination in post-endocytic compartments.

    Science.gov (United States)

    Fu, Lianwu; Rab, Andras; Tang, Li ping; Bebok, Zsuzsa; Rowe, Steven M; Bartoszewski, Rafal; Collawn, James F

    2015-01-01

    The ΔF508 mutant form of the cystic fibrosis transmembrane conductance regulator (ΔF508 CFTR) that is normally degraded by the ER-associated degradative pathway can be rescued to the cell surface through low-temperature (27°C) culture or small molecular corrector treatment. However, it is unstable on the cell surface, and rapidly internalized and targeted to the lysosomal compartment for degradation. To understand the mechanism of this rapid turnover, we examined the role of two adaptor complexes (AP-2 and Dab2) and three E3 ubiquitin ligases (c-Cbl, CHIP, and Nedd4-2) on low-temperature rescued ΔF508 CFTR endocytosis and degradation in human airway epithelial cells. Our results demonstrate that siRNA depletion of either AP-2 or Dab2 inhibits ΔF508 CFTR endocytosis by 69% and 83%, respectively. AP-2 or Dab2 depletion also increases the rescued protein half-life of ΔF508 CFTR by ~18% and ~91%, respectively. In contrast, the depletion of each of the E3 ligases had no effect on ΔF508 CFTR endocytosis, whereas CHIP depletion significantly increased the surface half-life of ΔF508 CFTR. To determine where and when the ubiquitination occurs during ΔF508 CFTR turnover, we monitored the ubiquitination of rescued ΔF508 CFTR during the time course of CFTR endocytosis. Our results indicate that ubiquitination of the surface pool of ΔF508 CFTR begins to increase 15 min after internalization, suggesting that CFTR is ubiquitinated in a post-endocytic compartment. This post-endocytic ubiquination of ΔF508 CFTR could be blocked by either inhibiting endocytosis, by siRNA knockdown of CHIP, or by treating cells with the CFTR corrector, VX-809. Our results indicate that the post-endocytic ubiquitination of CFTR by CHIP is a critical step in the peripheral quality control of cell surface ΔF508 CFTR.

  18. ΔF508 CFTR surface stability is regulated by DAB2 and CHIP-mediated ubiquitination in post-endocytic compartments.

    Directory of Open Access Journals (Sweden)

    Lianwu Fu

    Full Text Available The ΔF508 mutant form of the cystic fibrosis transmembrane conductance regulator (ΔF508 CFTR that is normally degraded by the ER-associated degradative pathway can be rescued to the cell surface through low-temperature (27°C culture or small molecular corrector treatment. However, it is unstable on the cell surface, and rapidly internalized and targeted to the lysosomal compartment for degradation. To understand the mechanism of this rapid turnover, we examined the role of two adaptor complexes (AP-2 and Dab2 and three E3 ubiquitin ligases (c-Cbl, CHIP, and Nedd4-2 on low-temperature rescued ΔF508 CFTR endocytosis and degradation in human airway epithelial cells. Our results demonstrate that siRNA depletion of either AP-2 or Dab2 inhibits ΔF508 CFTR endocytosis by 69% and 83%, respectively. AP-2 or Dab2 depletion also increases the rescued protein half-life of ΔF508 CFTR by ~18% and ~91%, respectively. In contrast, the depletion of each of the E3 ligases had no effect on ΔF508 CFTR endocytosis, whereas CHIP depletion significantly increased the surface half-life of ΔF508 CFTR. To determine where and when the ubiquitination occurs during ΔF508 CFTR turnover, we monitored the ubiquitination of rescued ΔF508 CFTR during the time course of CFTR endocytosis. Our results indicate that ubiquitination of the surface pool of ΔF508 CFTR begins to increase 15 min after internalization, suggesting that CFTR is ubiquitinated in a post-endocytic compartment. This post-endocytic ubiquination of ΔF508 CFTR could be blocked by either inhibiting endocytosis, by siRNA knockdown of CHIP, or by treating cells with the CFTR corrector, VX-809. Our results indicate that the post-endocytic ubiquitination of CFTR by CHIP is a critical step in the peripheral quality control of cell surface ΔF508 CFTR.

  19. Traffic Signs Inventory System

    Directory of Open Access Journals (Sweden)

    J. Ružbarský

    2013-06-01

    Full Text Available The paper is focused on practical application of Cambridge Correlator. The goal is to propose a traffic signs inventory system by using excellent characteristics of correlator in the rapid optical correlation. The proposal of this inventory system includes obtaining of traffic signs to create the database either collecting the GPS coordinates. It is necessary to know the traffic signs position and also to document the entire surface route for later evaluation in offline mode.

  20. Turning Traffic Jams to Jelly

    Institute of Scientific and Technical Information of China (English)

    2010-01-01

    Beijing, known for having the worst traffic in China, is brewing up a traffic plan with the harshest ever measures to ensure smooth traffic flow as the capital’s gridlock during rush hour and private car sales soar.

  1. Road Traffic in China

    NARCIS (Netherlands)

    Jie, L.; Van Zuylen, H.J.

    2014-01-01

    Traffic is tightly related to the social and economic development in a country. In China the development of the economy has been very fast in the past 30 years and this is still continuing. The transport infrastructure shows a similar pattern, while traffic is also rapidly growing. In urban areas th

  2. Distraction in traffic.

    NARCIS (Netherlands)

    2013-01-01

    Many drivers, as well as cyclists and pedestrians, are occupied with all kinds of activities that can distract their attention from traffic, like listening to music, conducting a conversation on their mobile phone, or texting. Distraction has negative effects on traffic behaviour. Most studies estim

  3. Traffic Light Options

    DEFF Research Database (Denmark)

    Jørgensen, Peter Løchte

    This paper introduces, prices, and analyzes traffic light options. The traffic light option is an innovative structured OTC derivative developed independently by several London-based investment banks to suit the needs of Danish life and pension (L&P) companies, which must comply with the traffic...... light solvency stress test system introduced by the Danish Financial Supervisory Authority (DFSA) in June 2001. This monitoring system requires L&P companies to submit regular reports documenting the sensitivity of the companies' base capital to certain pre-defined market shocks - the red and yellow...... light scenarios. These stress scenarios entail drops in interest rates as well as in stock prices, and traffic light options are thus designed to pay off and preserve sufficient capital when interest rates and stock prices fall simultaneously. Sweden's FSA implemented a traffic light system in January...

  4. Traffic Light Options

    DEFF Research Database (Denmark)

    Jørgensen, Peter Løchte

    2007-01-01

    This paper introduces, prices, and analyzes traffic light options. The traffic light option is an innovative structured OTC derivative developed independently by several London-based investment banks to suit the needs of Danish life and pension (L&P) companies, which must comply with the traffic...... light solvency stress test system introduced by the Danish Financial Supervisory Authority (DFSA) in June 2001. This monitoring system requires L&P companies to submit regular reports documenting the sensitivity of the companies' base capital to certain pre-defined market shocks - the red and yellow...... light scenarios. These stress scenarios entail drops in interest rates as well as in stock prices, and traffic light options are thus designed to pay off and preserve sufficient capital when interest rates and stock prices fall simultaneously. Sweden's FSA implemented a traffic light system in January...

  5. Properties of Traffic Risk Coefficient

    Institute of Scientific and Technical Information of China (English)

    TANG Tie-Qiao; HUANG Hai-Jun; SHANG Hua-Yan; XUE Yu

    2009-01-01

    We use the model with the consideration of the traffic interruption probability (Physica A 387(2008)6845) to study the relationship between the traffic risk coefficient and the traffic interruption probability.The analytical and numerical results show that the traffic interruption probability will reduce the traffic risk coefficient and that the reduction is related to the density, which shows that this model can improve traffic security.

  6. Syudy of Token Generation for Burst Traffic Shaping in Optical Burst Switching Networks

    Institute of Scientific and Technical Information of China (English)

    Tang Wan; So Won-ho; Lu Ji-guang; Kim Young-chon

    2004-01-01

    Traffic shaping is one of important control operation to guarantee the Quality of Service (QoS) in optical burst switching (OBS) networks. The efficiency of traffic shaping is mainly determined by token generation method. In this paper, token generation methods of traffic shaping are evaluated by using three kinds of probability distribution, and are analyzed in terms of burst blocking probability, throughput and correlation by simulation. The simulation results show that the token generation methods decrease the burst correlation of Label Switched Paths (LSPs), and solve traffic congestion as well. The different burst arrival processes have small impact on the blocking probability for OBS networks.

  7. Modes and regulation of endocytic membrane retrieval in mouse auditory hair cells.

    Science.gov (United States)

    Neef, Jakob; Jung, Sangyong; Wong, Aaron B; Reuter, Kirsten; Pangrsic, Tina; Chakrabarti, Rituparna; Kügler, Sebastian; Lenz, Christine; Nouvian, Régis; Boumil, Rebecca M; Frankel, Wayne N; Wichmann, Carolin; Moser, Tobias

    2014-01-15

    Synaptic vesicle recycling sustains high rates of neurotransmission at the ribbon-type active zones (AZs) of mouse auditory inner hair cells (IHCs), but its modes and molecular regulation are poorly understood. Electron microscopy indicated the presence of clathrin-mediated endocytosis (CME) and bulk endocytosis. The endocytic proteins dynamin, clathrin, and amphiphysin are expressed and broadly distributed in IHCs. We used confocal vglut1-pHluorin imaging and membrane capacitance (Cm) measurements to study the spatial organization and dynamics of IHC exocytosis and endocytosis. Viral gene transfer expressed vglut1-pHluorin in IHCs and targeted it to synaptic vesicles. The intravesicular pH was ∼6.5, supporting only a modest increase of vglut1-pHluorin fluorescence during exocytosis and pH neutralization. Ca(2+) influx triggered an exocytic increase of vglut1-pHluorin fluorescence at the AZs, around which it remained for several seconds. The endocytic Cm decline proceeded with constant rate (linear component) after exocytosis of the readily releasable pool (RRP). When exocytosis exceeded three to four RRP equivalents, IHCs additionally recruited a faster Cm decline (exponential component) that increased with the amount of preceding exocytosis and likely reflects bulk endocytosis. The dynamin inhibitor Dyngo-4a and the clathrin blocker pitstop 2 selectively impaired the linear component of endocytic Cm decline. A missense mutation of dynamin 1 (fitful) inhibited endocytosis to a similar extent as Dyngo-4a. We propose that IHCs use dynamin-dependent endocytosis via CME to support vesicle cycling during mild stimulation but recruit bulk endocytosis to balance massive exocytosis.

  8. Earthquake Model Confirms Traffic Jams Caused by Tiredness

    CERN Document Server

    Jarai-Szabo, Ferenc

    2011-01-01

    A simple one-dimensional spring-block model elaborated for the idealized single-lane highway traffic reveals the causes for the emergence of traffic jams. Based on the stop-time statistics of one car in the row, an order parameter is defined and studied. By extensive computer simulations, the parameter space of the model is explored, analyzed and interpreted. Existence of a free a and congested flow phases is confirmed and the transition between them is analyzed.

  9. From holoprosencephaly to osteopathology: role of multifunctional endocytic receptors in absorptive epithelia.

    Science.gov (United States)

    Müller, Dominik; Nykjaer, Anders; Willnow, Thomas E

    2003-01-01

    Megalin and cubilin are two multifunctional endocytic receptors expressed in many absorptive epithelia including the yolk sac, the renal proximal tubules, and the intestine. In these tissues, the receptors act in concert to mediate the cellular uptake of a variety of lipoproteins and vitamin/ carrier complexes. Recent studies in animal models and in patients suffering from receptor gene defects have highlighted the crucial role played by the receptors in systemic lipid and vitamin homeostasis, and the severe defects that result from receptor dysfunction. Here, we will review the molecular mechanisms that underlie normal receptor activity and that cause disease in the receptor-deficient organism.

  10. Evolutionary conservancy of the endocytic and trafficking machinery in the unicellular eukaryote Paramecium.

    Science.gov (United States)

    Surmacz, Liliana; Wiejak, Jolanta; Wyroba, Elzbieta

    2003-01-01

    Molecular search for the homologues of the mammalian proteins in the unicellular eukaryote Paramecium involved in endocytosis and membrane trafficking is discussed. We cloned and sequenced the gene fragments encoding the following components participating in endosome formation, sorting and maturation of the proprotein precursors, respectively, dynamin 2, Rab7 and furin. There is a proof that all these genes are expressed in this unicellular organism. The function of the identified immunoanalogues of the above described components of Paramecium endocytic machinery as well as a high degree of sequence homology to the respective human counterparts points to the evolutionary conservancy of these pathways.

  11. INTELLIGENT TRAFFIC-SAFETY MIRROR BY USING WIRELESS SENSOR NETWORK

    Directory of Open Access Journals (Sweden)

    Peter Danišovič

    2014-03-01

    Full Text Available This article is focused on the problematic of traffic safety, dealing with the problem of car intersections with blocked view crossing by a special wireless sensor network (WSN proposed for the traffic monitoring, concretely for vehicle’s detection at places, where it is necessary. Some ultra-low-power TI products were developed due to this reason: microcontroller MSP430F2232, 868MHz RF transceiver CC1101 and LDO voltage regulator TPS7033. The WSN consist of four network nodes supplied with the special safety lightings which serve the function of intelligent traffic safety mirror.

  12. Caenorhabditis elegans SAND-1 is essential for RAB-7 function in endosomal traffic.

    Science.gov (United States)

    Poteryaev, Dmitry; Fares, Hanna; Bowerman, Bruce; Spang, Anne

    2007-01-24

    The small rab-GTPase RAB-7 acts in endosome and endosome to lysosome traffic. We identified SAND-1 as a protein required for RAB-7 function based on similarities between SAND-1 and RAB-7 RNAi phenotypes. Although the initial uptake of yolk protein in oocytes, or of soluble secreted (ss) GFP in coelomocytes, appeared normal, further transport along the endocytic traffic route was delayed in the absence of SAND-1 function, and yolk proteins failed to reach yolk granules efficiently. Moreover, in coelomocytes, ssGFP and BSA-Texas-Red were endocytosed but not transported to lysosomes. We show that SAND-1 is essential for RAB-7 function at the transition from early to late endosomes, but not for RAB-7 function at lysosomes.

  13. Winning strategies in congested traffic

    CERN Document Server

    Jarai-Szabo, Ferenc

    2012-01-01

    One-directional traffic on two-lanes is modeled in the framework of a spring-block type model. A fraction $q$ of the cars are allowed to change lanes, following simple dynamical rules, while the other cars keep their initial lane. The advance of cars, starting from equivalent positions and following the two driving strategies is studied and compared. As a function of the parameter $q$ the winning probability and the average gain in the advancement for the lane-changing strategy is computed. An interesting phase-transition like behavior is revealed and conclusions are drawn regarding the conditions when the lane changing strategy is the better option for the drivers.

  14. Zitongxi Block

    Institute of Scientific and Technical Information of China (English)

    1996-01-01

    @@ Zitongxi Block (Western Zitong Block), is located in Zitong County, northwest of Sichuan Province (as shown on Fig. 8 ). Geologically. it is situated in the Zitong Depression, southwest of the middle Longmenshan faulted and folded belt, covering an area of 1 830 km2. Transportation is very convenient. A crisscross network of highways run through the block and the Baocheng railway is nearby. The climate is moderate. Most area belongs to hilly land with the elevation of 500-600 m.The Tongjiang River runs across the area.

  15. VBR video traffic models

    CERN Document Server

    Tanwir, Savera

    2014-01-01

    There has been a phenomenal growth in video applications over the past few years. An accurate traffic model of Variable Bit Rate (VBR) video is necessary for performance evaluation of a network design and for generating synthetic traffic that can be used for benchmarking a network. A large number of models for VBR video traffic have been proposed in the literature for different types of video in the past 20 years. Here, the authors have classified and surveyed these models and have also evaluated the models for H.264 AVC and MVC encoded video and discussed their findings.

  16. Road Traffic Injuries

    Institute of Scientific and Technical Information of China (English)

    WANG Zheng-guo

    2005-01-01

    @@ As everybody knows that automobiles have been greatly changing our life. However, everything has two sides, motor vehicles have also caused a huge number of people's deaths, injuries and property damage. Traffic crashes are perhaps the number one public health problem in developed countries [1]. In the United States, pre-retirement years of life lost in traffic crashes are more than that of the two combined leading diseases: cancer and heart disease [1]. Today road traffic crash (RTC) ranks 11th in leading cause of death and accounts for 2.1% of all deaths globally.

  17. SARS coronavirus entry into host cells through a novel clathrin- and caveolae-independent endocytic pathway

    Institute of Scientific and Technical Information of China (English)

    Hongliang Wang; Peng Yang; Kangtai Liu; Feng Guo; Yanli Zhang; Gongyi Zhang; Chengyu Jiang

    2008-01-01

    While severe acute respiratory syndrome coronavirus (SARS-CoV)fwas initially thought to enter cells through direct fusion with the plasma membrane, more recent evidence suggests that virus entry may also involve endocytosis. We have found that SARS-CoV enters cells via pH- and receptor-dependent endocytosis. Treatment of cells with either SARS-CoV spike protein or spike-bearing pseudoviruses resulted in the translocation of angiotensin-converting enzyme 2 (ACE2), the functional receptor of SARS-CoV, from the cell surface to endosomes. In addition, the spike-bearing pseudoviruses and early endosome antigen 1 were found to colocalize in endosomes. Further analyses using specific endocytic pathway inhibitors and dominant-negative Eps15 as well as caveolin-1 colocalization study suggested that virus entry was mediated by a clathrin- and caveolae-independent mechanism. Moreover, cholesterol- and sphingolipid-rich lipid raft microdomains in the plasma membrane, which have been shown to act as platforms for many physiological signaling pathways, were shown to be involved in virus entry. Endocytic entry of SARS-CoV may expand the cellular range of SARS-CoV infection, and our findings here contribute to the understanding of SARS-CoV pathogenesis, providing new information for anti-viral drug research.

  18. Rapid kinetics of endocytosis at rod photoreceptor synapses depends upon endocytic load and calcium.

    Science.gov (United States)

    Cork, Karlene M; Thoreson, Wallace B

    2014-05-01

    Release from rods is triggered by the opening of L-type Ca2+ channels that lie beneath synaptic ribbons. After exocytosis, vesicles are retrieved by compensatory endocytosis. Previous work showed that endocytosis is dynamin-dependent in rods but dynamin-independent in cones. We hypothesized that fast endocytosis in rods may also differ from cones in its dependence upon the amount of Ca2+ influx and/or endocytic load. We measured exocytosis and endocytosis from membrane capacitance (C m) changes evoked by depolarizing steps in voltage clamped rods from tiger salamander retinal slices. Similar to cones, the time constant for endocytosis in rods was quite fast, averaging endocytosis kinetics in rods slowed after increasing Ca2+ channel activation with longer step durations or more strongly depolarized voltage steps. Endocytosis kinetics also slowed as Ca2+ buffering was decreased by replacing BAPTA (10 or 1 mM) with the slower Ca2+ buffer EGTA (5 or 0.5 mM) in the pipette solution. These data provide further evidence that endocytosis mechanisms differ in rods and cones and suggest that endocytosis in rods is regulated by both endocytic load and local Ca2+ levels.

  19. The Endocytic Receptor Megalin and its Associated Proteins in Proximal Tubule Epithelial Cells

    Directory of Open Access Journals (Sweden)

    Shankhajit De

    2014-07-01

    Full Text Available Receptor-mediated endocytosis in renal proximal tubule epithelial cells (PTECs is important for the reabsorption and metabolization of proteins and other substances, including carrier-bound vitamins and trace elements, in glomerular filtrates. Impairment of this endocytic process results in the loss of such substances and development of proteinuria, which is an important clinical indicator of kidney diseases and is also a risk marker for cardiovascular disease. Megalin, a member of the low-density lipoprotein receptor gene family, is a multiligand receptor expressed in the apical membrane of PTECs and plays a central role in the endocytic process. Megalin interacts with various intracellular adaptor proteins for intracellular trafficking and cooperatively functions with other membrane molecules, including the cubilin-amnionless complex. Evidence suggests that megalin and the cubilin-amnionless complex are involved in the uptake of toxic substances into PTECs, which leads to the development of kidney disease. Studies of megalin and its associated molecules will be useful for future development of novel strategies for the diagnosis and treatment of kidney diseases.

  20. Potent Anti-HIV Chemokine Analogs Direct Post-Endocytic Sorting of CCR5.

    Directory of Open Access Journals (Sweden)

    Claudia Bönsch

    Full Text Available G protein-coupled receptors (GPCRs are desensitized and internalized following activation. They are then subjected to post-endocytic sorting (degradation, slow recycling or fast recycling. The majority of research on post-endocytic sorting has focused on the role of sequence-encoded address structures on receptors. This study focuses on trafficking of CCR5, a GPCR chemokine receptor and the principal entry coreceptor for HIV. Using Chinese Hamster Ovary cells stably expressing CCR5 we show that two different anti-HIV chemokine analogs, PSC-RANTES and 5P14-RANTES, direct receptor trafficking into two distinct subcellular compartments: the trans-Golgi network and the endosome recycling compartment, respectively. Our results indicate that a likely mechanism for ligand-directed sorting of CCR5 involves capacity of the chemokine analogs to elicit the formation of durable complexes of CCR5 and arrestin2 (beta-arrestin-1, with PSC-RANTES eliciting durable association in contrast to 5P14-RANTES, which elicits only transient association.

  1. Class III phosphoinositide 3-kinase/VPS34 and dynamin are critical for apical endocytic recycling.

    Science.gov (United States)

    Carpentier, Sarah; N'Kuli, Francisca; Grieco, Giuseppina; Van Der Smissen, Patrick; Janssens, Virginie; Emonard, Hervé; Bilanges, Benoît; Vanhaesebroeck, Bart; Gaide Chevronnay, Héloïse P; Pierreux, Christophe E; Tyteca, Donatienne; Courtoy, Pierre J

    2013-08-01

    Recycling is a limiting step for receptor-mediated endocytosis. We first report three in vitro or in vivo evidences that class III PI3K/VPS34 is the key PI3K isoform regulating apical recycling. A substractive approach, comparing in Opossum Kidney (OK) cells a pan-class I/II/III PI3K inhibitor (LY294002) with a class I/II PI3K inhibitor (ZSTK474), suggested that class III PI3K/VPS34 inhibition induced selective apical endosome swelling and sequestration of the endocytic receptor, megalin/LRP-2, causing surface down-regulation. GFP-(FYVE)x2 overexpression to sequester PI(3)P caused undistinguishable apical endosome swelling. In mouse kidney proximal tubular cells, conditional Vps34 inactivation also led to vacuolation and intracellular megalin redistribution. We next report that removal of LY294002 from LY294002-treated OK cells induced a spectacular burst of recycling tubules and restoration of megalin surface pool. Acute triggering of recycling tubules revealed recruitment of dynamin-GFP and dependence of dynamin-GTPase, guidance directionality by microtubules, and suggested that a microfilamentous net constrained endosomal swelling. We conclude that (i) besides its role in endosome fusion, PI3K-III is essential for endosome fission/recycling; and (ii) besides its role in endocytic entry, dynamin also supports tubulation of recycling endosomes. The unleashing of recycling upon acute reversal of PI3K inhibition may help study its dynamics and associated machineries.

  2. Estrogen effects on actin cytoskeletal and endocytic proteins associated with tubulobulbar complex disruption in rat testes.

    Science.gov (United States)

    Upadhyay, Rahul D; Kumar, Anita V; Sonawane, Shobha; Gaonkar, Reshma; Balasinor, Nafisa H

    2013-10-01

    Tubulobulbar complexes (TBCs), evaginations of mature spermatids, penetrate into the surrounding Sertoli cell cytoplasm of testis seminiferous epithelium during rat spermatogenesis. These structures prepare mature spermatids for their release into the seminiferous tubular lumen via a process called spermiation. Based on their functions of transient attachment and endocytosis, many actin-regulatory and endocytic proteins are associated with TBCs. Previously, exogenous 17β-estradiol administration to adult male rats showed spermiation failure that was attributed to TBC disruption. To determine the molecular basis of estrogen-induced TBC disruption, we examined the expressions and localizations of actin-regulatory proteins, endocytic proteins, Rho-GTPases, and phosphorylation in TBCs during sperm release. Results demonstrated absence of neural Wiscott Aldrich syndrome protein, cortactin, adaptor-related protein complex 2 sigma-1 subunit, dynamin 2, cell division control protein 42, and phosphocortactin in the concavity of spermatid head where TBCs are present without change in their protein expression levels. Absence of these proteins could have led to collapse of the TBC structure which is involved in its formation and function.

  3. The Endocytic Recycling Protein EHD2 Interacts with Myoferlin to Regulate Myoblast Fusion*

    Science.gov (United States)

    Doherty, Katherine R.; Demonbreun, Alexis R.; Wallace, Gregory Q.; Cave, Andrew; Posey, Avery D.; Heretis, Konstantina; Pytel, Peter; McNally, Elizabeth M.

    2008-01-01

    Skeletal muscle is a multinucleated syncytium that develops and is maintained by the fusion of myoblasts to the syncytium. Myoblast fusion involves the regulated coalescence of two apposed membranes. Myoferlin is a membrane-anchored, multiple C2 domain-containing protein that is highly expressed in fusing myoblasts and required for efficient myoblast fusion to myotubes. We found that myoferlin binds directly to the eps15 homology domain protein, EHD2. Members of the EHD family have been previously implicated in endocytosis as well as endocytic recycling, a process where membrane proteins internalized by endocytosis are returned to the plasma membrane. EHD2 binds directly to the second C2 domain of myoferlin, and EHD2 is reduced in myoferlin null myoblasts. In contrast to normal myoblasts, myoferlin null myoblasts accumulate labeled transferrin and have delayed recycling. Introduction of dominant negative EHD2 into myoblasts leads to the sequestration of myoferlin and inhibition of myoblast fusion. The interaction of myoferlin with EHD2 identifies molecular overlap between the endocytic recycling pathway and the machinery that regulates myoblast membrane fusion. PMID:18502764

  4. A CCR2 macrophage endocytic pathway mediates extravascular fibrin clearance in vivo

    Science.gov (United States)

    Motley, Michael P.; Madsen, Daniel H.; Jürgensen, Henrik J.; Spencer, David E.; Szabo, Roman; Holmbeck, Kenn; Flick, Matthew J.; Lawrence, Daniel A.; Castellino, Francis J.; Weigert, Roberto

    2016-01-01

    Extravascular fibrin deposition accompanies many human diseases and causes chronic inflammation and organ damage, unless removed in a timely manner. Here, we used intravital microscopy to investigate how fibrin is removed from extravascular space. Fibrin placed into the dermis of mice underwent cellular endocytosis and lysosomal targeting, revealing a novel intracellular pathway for extravascular fibrin degradation. A C-C chemokine receptor type 2 (CCR2)-positive macrophage subpopulation constituted the majority of fibrin-uptaking cells. Consequently, cellular fibrin uptake was diminished by elimination of CCR2-expressing cells. The CCR2-positive macrophage subtype was different from collagen-internalizing M2-like macrophages. Cellular fibrin uptake was strictly dependent on plasminogen and plasminogen activator. Surprisingly, however, fibrin endocytosis was unimpeded by the absence of the fibrin(ogen) receptors, αMβ2 and ICAM-1, the myeloid cell integrin-binding site on fibrin or the endocytic collagen receptor, the mannose receptor. The study identifies a novel fibrin endocytic pathway engaged in extravascular fibrin clearance and shows that interstitial fibrin and collagen are cleared by different subsets of macrophages employing distinct molecular pathways. PMID:26647393

  5. Endocytic regulation of voltage-dependent potassium channels in the heart.

    Science.gov (United States)

    Ishii, Kuniaki; Norota, Ikuo; Obara, Yutaro

    2012-01-01

    Understanding the regulation of cardiac ion channels is critical for the prevention of arrhythmia caused by abnormal excitability. Ion channels can be regulated by a change in function (qualitative) and a change in number (quantitative). Functional changes have been extensively investigated for many ion channels including cardiac voltage-dependent potassium channels. By contrast, the regulation of ion channel numbers has not been widely examined, particularly with respect to acute modulation of ion channels. This article briefly summarizes stimulus-induced endocytic regulation of major voltage-dependent potassium channels in the heart. The stimuli known to cause their endocytosis include receptor activation, drugs, and low extracellular [K(+)], following which the potassium channels undergo either clathrin-mediated or caveolin-mediated endocytosis. Receptor-mediated endocytic regulation has been demonstrated for Kv1.2, Kv1.5, KCNQ1 (Kv7.1), and Kv4.3, while drug-induced endocytosis has been demonstrated for Kv1.5 and hERG. Low [K(+)](o)-induced endocytosis might be unique for hERG channels, whose electrophysiological characteristics are known to be under strong influence of [K(+)](o). Although the precise mechanisms have not been elucidated, it is obvious that major cardiac voltage-dependent potassium channels are modulated by endocytosis, which leads to changes in cardiac excitability.

  6. Earthquake model describes traffic jams caused by imperfect driving styles

    Science.gov (United States)

    Járai-Szabó, Ferenc; Néda, Zoltán

    2012-11-01

    The wide modeling potential of the classical spring-block type system is illustrated by an interdisciplinary application. A simple one-dimensional spring-block chain with asymmetric spring forces is used to model idealized single-lane highway traffic and the emergence of phantom traffic jams. Based on the stop-time statistics of one car in the row, a proper order parameter is defined. By extensive computer simulations the parameter space of the model is explored, analyzed and interpreted. Existence of free and congested flow phases is confirmed and the transition between them is analyzed.

  7. Turning Traffic Jams to Jelly

    Institute of Scientific and Technical Information of China (English)

    LI LI

    2010-01-01

    @@ Beijing, known for having the worst traffic in China, is brewing up a traffic plan with the harshest ever measures to ensure smooth traffic flow as the capital's gridlock during rush hour and private car sales soar. On December 13, the Beijing Municipal Government started soliciting public opinions for a draft plan designed to relieve the city's traffic problems.

  8. Allegheny County Traffic Counts

    Data.gov (United States)

    Allegheny County / City of Pittsburgh / Western PA Regional Data Center — Traffic sensors at over 1,200 locations in Allegheny County collect vehicle counts for the Pennsylvania Department of Transportation. Data included in the Health...

  9. Ethernet traffic measurement

    OpenAIRE

    Santschi, Yann; Corthay, François

    2012-01-01

    The goal of this project is to create a FPGA Ethernet traffic measurement point and interface it with an existing measurement network, named DPMI, that is developed in Blekinge Institute of Technology.

  10. Non-Traffic Citations

    Data.gov (United States)

    Allegheny County / City of Pittsburgh / Western PA Regional Data Center — Non-traffic citations (NTCs, also known as "summary offenses") document low-level criminal offenses where a law enforcement officer or other authorized official...

  11. Cdc42-dependent Modulation of Tight Junctions and Membrane Protein Traffic in Polarized Madin-Darby Canine Kidney Cells

    Science.gov (United States)

    Rojas, Raul; Ruiz, Wily G.; Leung, Som-Ming; Jou, Tzuu-Shuh; Apodaca, Gerard

    2001-01-01

    Polarized epithelial cells maintain the asymmetric composition of their apical and basolateral membrane domains by at least two different processes. These include the regulated trafficking of macromolecules from the biosynthetic and endocytic pathway to the appropriate membrane domain and the ability of the tight junction to prevent free mixing of membrane domain-specific proteins and lipids. Cdc42, a Rho family GTPase, is known to govern cellular polarity and membrane traffic in several cell types. We examined whether this protein regulated tight junction function in Madin-Darby canine kidney cells and pathways that direct proteins to the apical and basolateral surface of these cells. We used Madin-Darby canine kidney cells that expressed dominant-active or dominant-negative mutants of Cdc42 under the control of a tetracycline-repressible system. Here we report that expression of dominant-active Cdc42V12 or dominant-negative Cdc42N17 altered tight junction function. Expression of Cdc42V12 slowed endocytic and biosynthetic traffic, and expression of Cdc42N17 slowed apical endocytosis and basolateral to apical transcytosis but stimulated biosynthetic traffic. These results indicate that Cdc42 may modulate multiple cellular pathways required for the maintenance of epithelial cell polarity. PMID:11514615

  12. CD163-L1 is an endocytic macrophage protein strongly regulated by mediators in the inflammatory response

    DEFF Research Database (Denmark)

    Moeller, Jesper B; Nielsen, Marianne J; Reichhardt, Martin P

    2012-01-01

    -phase mediator IL-6 and the anti-inflammatory mediator IL-10 but is suppressed by the proinflammatory mediators IL-4, IL-13, TNF-α, and LPS/IFN-γ. Furthermore, we show that CD163-L1 is an endocytic receptor, which internalizes independently of cross-linking through a clathrin-mediated pathway. Two cytoplasmic...

  13. Megalin and cubilin are endocytic receptors involved in renal clearance of hemoglobin

    DEFF Research Database (Denmark)

    Gburek, Jakub; Verroust, Pierre J; Willnow, Thomas E

    2002-01-01

    The kidney is the main site of hemoglobin clearance and degradation in conditions of severe hemolysis. Herein it is reported that megalin and cubilin, two epithelial endocytic receptors, mediate the uptake of hemoglobin in renal proximal tubules. Both receptors were purified by use of hemoglobin......-Sepharose affinity chromatography of solubilized renal brush-border membranes. Apparent dissociation constants of 1.7 microM for megalin and 4.1 microM for cubilin were determined by surface plasmon resonance analysis. The binding was calcium dependent in both cases. Uptake of fluorescence-labeled hemoglobin by BN...... not affect the uptake. By use of immunohistochemistry, it was demonstrated that uptake of hemoglobin in proximal tubules of rat, mouse, and dog kidneys occurs under physiologic conditions. Studies on normal and megalin knockout mouse kidney sections showed that megalin is responsible for physiologic...

  14. Stem-cell-specific endocytic degradation defects lead to intestinal dysplasia in Drosophila

    Directory of Open Access Journals (Sweden)

    Péter Nagy

    2016-05-01

    Full Text Available UV radiation resistance-associated gene (UVRAG is a tumor suppressor involved in autophagy, endocytosis and DNA damage repair, but how its loss contributes to colorectal cancer is poorly understood. Here, we show that UVRAG deficiency in Drosophila intestinal stem cells leads to uncontrolled proliferation and impaired differentiation without preventing autophagy. As a result, affected animals suffer from gut dysfunction and short lifespan. Dysplasia upon loss of UVRAG is characterized by the accumulation of endocytosed ligands and sustained activation of STAT and JNK signaling, and attenuation of these pathways suppresses stem cell hyperproliferation. Importantly, the inhibition of early (dynamin-dependent or late (Rab7-dependent steps of endocytosis in intestinal stem cells also induces hyperproliferation and dysplasia. Our data raise the possibility that endocytic, but not autophagic, defects contribute to UVRAG-deficient colorectal cancer development in humans.

  15. Endocytic structures and synaptic vesicle recycling at a central synapse in awake rats.

    Science.gov (United States)

    Körber, Christoph; Horstmann, Heinz; Sätzler, Kurt; Kuner, Thomas

    2012-12-01

    The synaptic vesicle (SV) cycle has been studied extensively in cultured cells and slice preparations, but not much is known about the roles and relative contributions of endocytic pathways and mechanisms of SV recycling in vivo, under physiological patterns of activity. We employed horseradish peroxidase (HRP) as an in vivo marker of endocytosis at the calyx of Held synapse in the awake rat. Ex vivo serial section scanning electron microscopy and 3D reconstructions revealed two categories of labelled structures: HRP-filled SVs and large cisternal endosomes. Inhibition of adaptor protein complexes 1 and 3 (AP-1, AP-3) by in vivo application of Brefeldin A (BFA) disrupted endosomal SV budding while SV recycling via clathrin-mediated endocytosis (CME) remained unaffected. In conclusion, our study establishes cisternal endosomes as an intermediate of the SV cycle and reveals CME and endosomal budding as the predominant mechanisms of SV recycling in a tonically active central synapse in vivo.

  16. The tandem endocytic receptors megalin and cubilin are important proteins in renal pathology.

    Science.gov (United States)

    Verroust, Pierre J; Birn, Henrik; Nielsen, Rikke; Kozyraki, Renata; Christensen, Erik Ilsø

    2002-09-01

    The molecular mechanisms controlling proximal tubule reabsorption of proteins have been much elucidated in recent years. Megalin and cubilin constitute two important endocytic receptor proteins involved in this process. Although structurally very different the two receptor proteins interact to mediate the reabsorption of a large number of filtered proteins, including carrier proteins important for transport and cellular uptake of several vitamins, lipids and other nutrients. Dysfunction of either protein results in tubular proteinuria and is associated with specific changes in vitamin metabolism due to the defective proximal tubular reabsorption of carrier proteins. Additional focus on the two receptors is attracted by the possible pathogenic role of excessive tubular protein uptake during conditions of increased filtration of proteins, and by recent findings implicating members of the low density lipoprotein-receptor family, which includes megalin, in the transduction of signals by association with cytoplasmic proteins.

  17. Endocytic proteins drive vesicle growth via instability in high membrane tension environment

    CERN Document Server

    Walani, Nikhil; Agrawal, Ashutosh

    2015-01-01

    Clathrin-mediated endocytosis (CME) is a key pathway for transporting cargo into cells via membrane vesicles. It plays an integral role in nutrient import, signal transduction, neurotransmission and cellular entry of pathogens and drug-carrying nanoparticles. As CME entails substantial local remodeling of the plasma membrane, the presence of membrane tension offers resistance to bending and hence, vesicle formation. Experiments show that in such high tension conditions, actin dynamics is required to carry out CME successfully. In this study, we build upon these pioneering experimental studies to provide fundamental mechanistic insights into the roles of two key endocytic proteins, namely, actin and BAR proteins in driving vesicle formation in high membrane tension environment. Our study reveals a new actin force induced `snap-through instability' that triggers a rapid shape transition from a shallow invagination to a highly invaginated tubular structure. We show that the association of BAR proteins stabilizes...

  18. Localization of HCMV UL33 and US27 in endocytic compartments and viral membranes

    DEFF Research Database (Denmark)

    Fraile-Ramos, Alberto; Pelchen-Matthews, Annegret; Kledal, Thomas N

    2002-01-01

    The human cytomegalovirus genome encodes four putative seven transmembrane domain chemokine receptor-like proteins. Although important in viral pathogenesis, little is known about the properties or functions of these proteins. We previously reported that US28 is located in endocytic vesicles...... suggests that the localization of UL33, US27 and US28 to endosomes may allow these proteins to be incorporated into the viral membrane during the final stages of human cytomegalovirus assembly....... and undergoes constitutive endocytosis and recycling. Here we studied the cellular distributions and trafficking of two other human cytomegalovirus chemokine receptor-like proteins, UL33 and US27, in transfected and human cytomegalovirus-infected cells. Immunofluorescence staining indicated that UL33 and US27...

  19. Pollen Tube Growth: a Delicate Equilibrium Between Secretory and Endocytic Pathways

    Institute of Scientific and Technical Information of China (English)

    Alessandra Moscatelli; Aurora Irene Idilli

    2009-01-01

    Although pollen tube growth is a prerequisite for higher plant fertilization and seed production, the processes leading to pollen tube emission and elongation are crucial for understanding the basic mechanisms of tip growth. It was generally accepted that pollen tube elongation occurs by accumulation and fusion of Golgi-derived secretory vesicles (SVs) in the apical region, or clear zone, where they were thought to fuse with a restricted area of the apical plasma membrane (PM), defining the apical growth domain. Fusion of SVs at the tip reverses outside cell wall material and provides new segments of PM. However, electron microscopy studies have clearly shown that the PM Incorporated at the tip greatly exceeds elongation and a mechanism of PM retrieval was already postulated in the mid-nineteenth century. Recent studies on endocytosis during pollen tube growth showed that different endocytic pathways occurred in distinct zones of the tube, including the apex, and led to a new hypothesis to explain vesicle accumulation at the tip; namely, that en-docytic vesicles contribute substantially to V-shaped vesicle accumulation in addition to SVs and that exocytosis does not involve the entire apical domain. New insights suggested the intriguing hypothesis that modulation between exo-and endocytosis in the apex contributes to maintain PM polarity in terms of lipid/protein composition and showed distinct degradation pathways that could have different functions in the physiology of the call. Pollen tube growth in vivo is closely regulated by interaction with style molecules. The study of endocytosis and membrane recycling in pollen tubes opens new perspectives to studying pollen tube-style interactions in vivo.

  20. Renal uptake of myoglobin is mediated by the endocytic receptors megalin and cubilin.

    Science.gov (United States)

    Gburek, Jakub; Birn, Henrik; Verroust, Pierre J; Goj, Bogusława; Jacobsen, Christian; Moestrup, Søren K; Willnow, Thomas E; Christensen, Erik I

    2003-09-01

    Nephrotoxicity of myoglobin is well recognized as playing a part in the development of acute renal failure in settings of myoglobinuria. However, the molecular mechanism of myoglobin uptake in renal proximal tubules has not been clarified. Here, we report that the endocytic receptors megalin and cubilin are involved in renal reabsorption of myoglobin. Both receptors were captured from solubilized renal brush-border membranes by affinity chromatography using myoglobin-Sepharose. Myoglobin bound to purified megalin and cubilin with Kd values of 2.0 and 3 microM, respectively, as evaluated by surface plasmon resonance analysis. Apomyoglobin bound to megalin with the same affinity, and the affinity of apomyoglobin to cubilin was reduced (Kd = 5 microM). Radioiodinated myoglobin could be displaced by apomyoglobin in inhibition studies using isolated renal brush-border membranes (Ki approximately 2 microM). Receptor-associated protein as well as antibodies directed against megalin and cubilin markedly inhibited the uptake of fluorescent-labeled myoglobin by cultured yolk sac BN-16 cells. The significance of megalin- and cubilin-mediated endocytosis for myoglobin uptake in vivo was demonstrated by use of kidney-specific megalin knockout mice. Injected myoglobin was extensively reabsorbed by megalin-expressing proximal tubular cells, whereas there was very little uptake in the megalin-deficient cells. In conclusion, this study establishes the molecular mechanism of myoglobin uptake in the renal proximal tubule involving the endocytic receptors megalin and cubilin. Identification of the receptors for tubular uptake of myoglobin may be essential for development of new therapeutic strategies for myoglobinuric acute renal failure.

  1. Megalin and cubilin are endocytic receptors involved in renal clearance of hemoglobin.

    Science.gov (United States)

    Gburek, Jakub; Verroust, Pierre J; Willnow, Thomas E; Fyfe, John C; Nowacki, Wojciech; Jacobsen, Christian; Moestrup, Søren K; Christensen, Erik I

    2002-02-01

    The kidney is the main site of hemoglobin clearance and degradation in conditions of severe hemolysis. Herein it is reported that megalin and cubilin, two epithelial endocytic receptors, mediate the uptake of hemoglobin in renal proximal tubules. Both receptors were purified by use of hemoglobin-Sepharose affinity chromatography of solubilized renal brush-border membranes. Apparent dissociation constants of 1.7 microM for megalin and 4.1 microM for cubilin were determined by surface plasmon resonance analysis. The binding was calcium dependent in both cases. Uptake of fluorescence-labeled hemoglobin by BN-16 cells was inhibited by anti-megalin and anti-cubilin antibodies as well as by receptor-associated protein, a chaperone for LDL-receptor family proteins. Partial inhibition by myoglobin was observed, whereas bovine serum albumin, intrinsic factor-cobalamin complexes, and beta2-microglobulin did not affect the uptake. By use of immunohistochemistry, it was demonstrated that uptake of hemoglobin in proximal tubules of rat, mouse, and dog kidneys occurs under physiologic conditions. Studies on normal and megalin knockout mouse kidney sections showed that megalin is responsible for physiologic clearance of hemoglobin. Labeling intensities in kidneys from normal and cubilin-malexpressing dogs were similar, which suggests that, in the normal state, the role of cubilin in uptake of hemoglobin is rather limited. However, cubilin is likely to assist hemoglobin endocytosis in settings of hemoglobinuria. In conclusion, the study provides a molecular explanation for long-standing observations of hemoglobin uptake in renal proximal tubules that involve the endocytic receptors megalin and cubilin. The findings may prove to be essential for further research on the pathophysiology of hemoglobinuric acute renal failure and proteinuria-associated tubulointerstitial nephritis.

  2. Interactions between Rab and Arf GTPases regulate endosomal phosphatidylinositol-4,5-bisphosphate during endocytic recycling.

    Science.gov (United States)

    Shi, Anbing; Grant, Barth D

    2013-01-01

    After endocytosis, a selective endocytic recycling process returns many endocytosed molecules back to the plasma membrane. The RAB-10/Rab10 GTPase is known to be a key recycling regulator for specific cargo molecules. New evidence, focused on C. elegans RAB-10 in polarized epithelia, points to a key role of RAB-10 in the regulation of endosomal phosphatidylinositol-4,5-bisphosphate (PI(4,5)P2) levels. In turn, PI(4,5)P2 levels strongly influence the recruitment of many peripheral membrane proteins, including those important for vesicle budding through their membrane bending activities. Part of the effect of RAB-10 on endosomal PI(4,5)P2 is through its newly identified effector CNT-1, a predicted GTPase activating protein (GAP) of the small GTPase ARF-6/Arf6. In mammals PI(4,5)P2 generating enzymes are known Arf6 effectors. In C. elegans we found that RAB-10, CNT-1 and ARF-6 are present on the same endosomes, that RAB-10 recruits CNT-1 to endosomes, and that loss of CNT-1 or RAB-10 leads to overaccumulation of endosomal PI(4,5)P2, presumably via hyperactivation of endosomal ARF-6. In turn this leads to over-recruitment of PI(4,5)P2-dependent membrane-bending proteins RME-1/Ehd and SDPN-1/Syndapin/PACSIN. Conversely, in arf-6 mutants, endosomal PI(4,5)P2 levels were reduced and endosomal recruitment of RME-1 and SDPN-1 failed. This work makes an unexpected link between distinct classes of small GTPases that control endocytic recycling, and provides insight into how this interaction affects endosome function at the level of lipid phosphorylation.

  3. Energy Saving Scheme Based On Traffic Forwarding For Optical Fiber Access Networks

    DEFF Research Database (Denmark)

    Lopez, G. Arturo Rodes; Estaran Tolosa, Jose Manuel; Vegas Olmos, Juan José;

    2013-01-01

    We report on an energy saving block that regroups and powers off OLTs during low traffic periods, resulting in energy savings up to 87,5% in the central office of optical access networks.......We report on an energy saving block that regroups and powers off OLTs during low traffic periods, resulting in energy savings up to 87,5% in the central office of optical access networks....

  4. Transient handover blocking probabilities in road covering cellular mobile networks

    NARCIS (Netherlands)

    Boucherie, Richard J.; Wal, van der Jan

    2003-01-01

    This paper investigates handover and fresh call blocking probabilities for subscribers moving along a road in a traffic jam passing through consecutive cells of a wireless network. It is observed and theoretically motivated that the hand- over blocking probabilities show a sharp peak in the initial

  5. Transient handover blocking probabilities in road covering cellular mobile networks

    NARCIS (Netherlands)

    Boucherie, R.J.; Wal, van der J.

    2002-01-01

    This paper investigates handover and fresh call blocking probabilities for subscribers moving along a road in a traffic jam passing through consecutive cells of a wireless network. It is observed and theoretically motivated that the handover blocking probabilities show a sharp peak in the initial pa

  6. Alternate Routing in Tandem Traffic-Groomed Optical Networks

    Directory of Open Access Journals (Sweden)

    Alicia Nicki Washington

    2007-09-01

    Full Text Available Recent advances in telecommunication networks have allowed WDM to emerge as a viable solution to the ever-increasing demands of the Internet. Because these networks carry large amounts of traffic, alternate routing methods are designed in order to allow traffic to be properly re-routed from source to destination in the event of certain events, such as link blocking or failure. In this paper, we consider a tandem traffic-groomed optical network, modeled as a multi-level overflow system, where each level represents a wavelength between adjacent nodes. The queueing network is analyzed using a combination of methods. As will be shown, the decomposition method provides a good approximate analysis of large overflow systems supporting traffic from multiple sources.

  7. 14 CFR Section 25 - Traffic and Capacity Elements

    Science.gov (United States)

    2010-01-01

    ... these joint-service operations are: Blocked-space agreements; part-charter agreements; code-sharing agreements; wet-lease agreements, and other similar arrangements. (i) Joint-service operations are reported... under its own FAA operating authority. The traffic moving under these agreements is reported on...

  8. Effects of traffic infrastructure and road design on safe driving

    NARCIS (Netherlands)

    Horst, A.R.A. van der

    2014-01-01

    One of the building blocks of the Dutch success in road safety is the Sustainable Safety approach. This philosophy specifies that road safety should be a design requirement in road traffic with a focus on the use of human characteristics as a starting point, considering both humans’ physical vulnera

  9. Traffic Light Detection

    DEFF Research Database (Denmark)

    Philipsen, Mark Philip; Jensen, Morten Bornø; Møgelmose, Andreas

    2015-01-01

    Traffic light recognition (TLR) is an integral part of any intelligent vehicle, which must function in the existing infrastructure. Pedestrian and sign detection have recently seen great improvements due to the introduction of learning based detectors using integral channel features. A similar push...... database is collected based on footage from US roads. The database consists of both test and training data, totaling 46,418 frames and 112,971 annotated traffic lights, captured in continuous sequences under a varying light and weather conditions. The learning based detector achieves an AUC of 0.4 and 0...

  10. Generalized Deterministic Traffic Rules

    CERN Document Server

    Fuks, H; Fuks, Henryk; Boccara, Nino

    1997-01-01

    We study a family of deterministic models for highway traffic flow which generalize cellular automaton rule 184. This family is parametrized by the speed limit $m$ and another parameter $k$ that represents a ``degree of aggressiveness'' in driving, strictly related to the distance between two consecutive cars. We compare two driving strategies with identical maximum throughput: ``conservative'' driving with high speed limit and ``aggressive'' driving with low speed limit. Those two strategies are evaluated in terms of accident probability. We also discuss fundamental diagrams of generalized traffic rules and examine limitations of maximum achievable throughput. Possible modifications of the model are considered.

  11. Vps1 in the late endosome-to-vacuole traffic

    Indian Academy of Sciences (India)

    Jacob Hayden; Michelle Williams; Ann Granich; Hyoeun Ahn; Brandon Tenay; Joshua Lukehart; Chad Highfill; Sarah Dobard; Kyoungtae Kim

    2013-03-01

    Vacuolar protein sorting 1 (Vps1), the yeast homolog to human dynamin, is a GTP hydrolyzing protein, which plays an important role in protein sorting and targeting between the Golgi and late endosomal compartments. In this study, we assessed the functional significance of Vps1 in the membrane traffic towards the vacuole. We show here that vps1 cells accumulated FM4-64 to a greater extent than wild-type (WT) cells, suggesting slower endocytic degradation traffic toward the vacuole. In addition, we observed that two endosome-to-vacuole traffic markers, DsRed-FYVE and Ste2-GFP, were highly accumulated in Vps1-deficient cells, further supporting Vps1’s implication in efficient trafficking of endocytosed materials to the vacuole. Noteworthy, a simultaneous imaging analysis in conjunction with FM4-64 pulse-chase experiment further revealed that Vps1 plays a role in late endosome to the vacuole transport. Consistently, our subcellular localization analysis showed that Vps1 is present at the late endosome. The hyperaccumulation of endosomal intermediates in the vps1 mutant cells appears to be caused by the disruption of integrity of HOPS tethering complexes, manifested by mislocalization of Vps39 to the cytoplasm. Finally, we postulate that Vps1 functions together with the Endosomal Sorting Complex Required for Transport (ESCRT) complex at the late endosomal compartments, based on the observation that the double mutants, in which VPS1 along with singular ESCRT I, II and III genes have been disrupted, exhibited synthetic lethality. Together, we propose that Vps1 is required for correct and efficient trafficking from the late endosomal compartments to the vacuole.

  12. 2013 Traffic Safety Culture Index

    Science.gov (United States)

    ... death in the United States. 2013 Traffic Safety Culture Index January 2014 607 14th Street, NW, Suite ... org | 202-638-5944 Title 2013 Traffic Safety Culture Index (January 2014) About the Sponsor AAA Foundation ...

  13. Chaotic Control of Network Traffic

    Institute of Scientific and Technical Information of China (English)

    YANG Tan; CUI Yi-Dong; JIN Yue-Hui; CHENG Shi-Duan

    2009-01-01

    A method of chaotic control on network traffic is presented.By this method,the chaotic network traffic can be controlled to a pre-assigned equilibrium point according to chaotic prediction and the largest Lyapunov exponent of the traffic on congested link is reduced,thereby the probability of traffic burst and network congestion can be reduced.Numerical examples show that this method is effective.

  14. The Classroom Traffic Jam

    Science.gov (United States)

    Edwards, Arthur W.

    1977-01-01

    The importance of energy conservation is developed in this simulation. Children draw an automobile and then are asked to drive it through the classroom roadways. When a traffic jam results, students offer ways to eliminate it. The importance of mass transportation and car pools is stressed by the teacher. (MA)

  15. Cyclists' Injuries in Traffic

    Directory of Open Access Journals (Sweden)

    Eduard Missoni

    2004-01-01

    Full Text Available Among numerous participants in road traffic there are alsothe cyclists. Cycling has become during the recent years one ofthe major components of the modem living, even in Croatia. Inalmost all the developed countries of the world, cycling isemphasised as an activity which improves health and contributesto the improvement of overall physical fitness and environmentalprotection. During the period between 1 January 1996and 31 December 1999, an analysis of the number of cyclist accidentsin road traffic was carried out. Special attention waspaid to the number of injured cyclists. In 1996, in road traffic,there were 1,218 cyclist accidents in which 912 (74.9% cyclistswere injured. In 1997, there were 1,146 incidents and accidents,with 854 (74.5% injured persons. In 1998, there were 1,167accidents, 887 (76.0% injured. In the last study in 1999, thetotal number of cyclist accidents amounted to 1,230, and thenumber of the injured cyclists was 924 (75.1% .Every event resulting in a more or less severe disability is agreat distress and great damage to every injured person as individualas well as for the family and eventually for the society asa whole. This paper recommends certain measures and proceduresthat should substantially reduce the traffic accidents andincidents which involve cyclists.

  16. Air Traffic Flow Management

    CERN Document Server

    Ganu, Hrishikesh V

    2008-01-01

    Air Traffic Flow Management is the regulation of air traffic in order to avoid exceeding airport or flight sector capacity in handling traffic, and to ensure that available capacity is used efficiently. We have tried to explore the logic behind the claims by Bertsimas et.al about integral solutions to the LP relaxation of the Traffic Flow Management Problem(TFMP). Polyhedral theory only indicates that the stronger TFMP formulation of Bertsimas et.al might lead to integral solutions in some cases. Our computations indicate that the encouraging results reported by Bertsimas et.al are not merely fortuitous or due to their specific data set. Indeed, we found that the TFMP had integral solutions even in case of artificial data sets generated to include severe conflicts in the flight schedules. In our limited tests with 4-5 scenarios, we obtained non-integral solutions only once. This is of significant practical importance because, the LP relaxation can be solved even on small machines with low memory and processor...

  17. Study and Simulation of Traffic Behavior in Cellular Network

    Science.gov (United States)

    Madhup, D. K.; Shrestha, C. L.; Sharma, R. K.

    2007-07-01

    Cellular radio systems accommodate a large number of users with a limited radio spectrum. The concept of trunking allows a large number of users to share the relatively small number of channels in a cell by providing access to each user, on demand, from a pool of available channels. Traffic engineering deals with provisioning of communication circuits in a given area for a number of subscribers with a required grade of service. Traffic in any cell depends upon the number of users, the average request rate and average call duration. Certain number of channels is required for the required GOS. To design an optimum capacity cellular system, traffic behavior on that system is important. The number of channel required can be estimated by using Erlang formula and Erlang table. Erlang table is not always useful to calculate the probability of blocking in various complex scenarios such as channel borrowing strategies. When the total number of channel available in a given cell are divided to serve partly for newly generated calls and partly for handover calls, and if they use dynamic channel assignment strategies like channel borrowing, then the probability of blocking can't be calculated from Erlang table. Simulation model of the behavior help us to determine the blocking and the channel utilization while using various channel assignment strategies. The title "Study and Simulation of Traffic Behavior in Cellular Network" entail the study of the blocking probability of traffic in cellular network for static channel assignment strategies and dynamic channel borrowing strategies through MATLAB programming language and graphic user interface (GUI). The result shows that the dynamic scheme can perform better than static maximizing the overall utilization of the circuits and minimizing the overall blocking.

  18. ITSA: Internet Traffic Statistics Archive

    NARCIS (Netherlands)

    Hoogesteger, Martijn; Oliveira Schmidt, de Ricardo; Pras, Aiko

    2016-01-01

    Motivated by the fact that comprehensive and long term Internet traffic measurements can be hard to obtain, we have proposed and developed the Internet Traffic Statistics Archive (ITSA). Since 2013, ITSA concentrates reports on high-level statistics of Internet traffic worldwide. Examples of statist

  19. Pedalcylists. Traffic Safety Facts, 2000.

    Science.gov (United States)

    National Highway Traffic Safety Administration (DOT), Washington, DC.

    This document provides statistical information on traffic accidents involving U.S. bicyclists. Data include: (1) trends in pedalcyclist and total traffic fatalities, 1990-2000; (2) non-occupant traffic fatalities, 1990-2000; (3) pedalcyclists killed and injured, and fatality and injury rates, by age and sex, 2000; and (4) pedalcyclist traffic…

  20. Effects of Traffic Lights on CA Traffic Model

    Institute of Scientific and Technical Information of China (English)

    SuweiFENG; GuoqingGU

    1997-01-01

    Cellular automaton traffic models can include various factors in traffic system with simple regulations and the corresponding computations and simulations are rather convenient and effective.In this paper,the Biham-Middleton-Levine model(briefly saying the BML model)is improved by removing its limitation of synchronized change of traffic lights.In new model,the traffic light at each crossing could arbitrarily change its starting time and tempo of variation and hence the model could more realistically describe the influence of traffic lights on the performance of traffic systems.The cases of traffic light with unsynchronized starting time and different tempo of variation are simulated in this paper.

  1. Membrane trafficking. The specificity of vesicle traffic to the Golgi is encoded in the golgin coiled-coil proteins.

    Science.gov (United States)

    Wong, Mie; Munro, Sean

    2014-10-31

    The Golgi apparatus is a multicompartment central sorting station at the intersection of secretory and endocytic vesicular traffic. The mechanisms that permit cargo-loaded transport vesicles from different origins to selectively access different Golgi compartments are incompletely understood. We developed a rerouting and capture assay to investigate systematically the vesicle-tethering activities of 10 widely conserved golgin coiled-coil proteins. We find that subsets of golgins with distinct localizations on the Golgi surface have capture activities toward vesicles of different origins. These findings demonstrate that golgins act as tethers in vivo, and hence the specificity we find to be encoded in this tethering is likely to make a major contribution to the organization of membrane traffic at the Golgi apparatus.

  2. Evolutionary Concepts for Decentralized Air Traffic Flow Management

    Science.gov (United States)

    Adams, Milton; Kolitz, Stephan; Milner, Joseph; Odoni, Amedeo

    1997-01-01

    Alternative concepts for modifying the policies and procedures under which the air traffic flow management system operates are described, and an approach to the evaluation of those concepts is discussed. Here, air traffic flow management includes all activities related to the management of the flow of aircraft and related system resources from 'block to block.' The alternative concepts represent stages in the evolution from the current system, in which air traffic management decision making is largely centralized within the FAA, to a more decentralized approach wherein the airlines and other airspace users collaborate in air traffic management decision making with the FAA. The emphasis in the discussion is on a viable medium-term partially decentralized scenario representing a phase of this evolution that is consistent with the decision-making approaches embodied in proposed Free Flight concepts for air traffic management. System-level metrics for analyzing and evaluating the various alternatives are defined, and a simulation testbed developed to generate values for those metrics is described. The fundamental issue of modeling airline behavior in decentralized environments is also raised, and an example of such a model, which deals with the preservation of flight bank integrity in hub airports, is presented.

  3. Cubilin, the endocytic receptor for intrinsic factor-vitamin B(12) complex, mediates high-density lipoprotein holoparticle endocytosis.

    Science.gov (United States)

    Hammad, S M; Stefansson, S; Twal, W O; Drake, C J; Fleming, P; Remaley, A; Brewer, H B; Argraves, W S

    1999-08-31

    Receptors that endocytose high-density lipoproteins (HDL) have been elusive. Here yolk-sac endoderm-like cells were used to identify an endocytic receptor for HDL. The receptor was isolated by HDL affinity chromatography and identified as cubilin, the recently described endocytic receptor for intrinsic factor-vitamin B(12). Cubilin antibodies inhibit HDL endocytosis by the endoderm-like cells and in mouse embryo yolk-sac endoderm, a prominent site of cubilin expression. Cubilin-mediated HDL endocytosis is inhibitable by HDL(2), HDL(3), apolipoprotein (apo)A-I, apoA-II, apoE, and RAP, but not by low-density lipoprotein (LDL), oxidized LDL, VLDL, apoC-I, apoC-III, or heparin. These findings, coupled with the fact that cubilin is expressed in kidney proximal tubules, suggest a role for this receptor in embryonic acquisition of maternal HDL and renal catabolism of filterable forms of HDL.

  4. Virtualized Traffic at Metropolitan Scales

    Directory of Open Access Journals (Sweden)

    David eWilkie

    2015-05-01

    Full Text Available Few phenomena are more ubiquitous than traffic, and few are more significant economically, socially, or environmentally. Any virtual reality system, including virtual globes and immersive multi-player worlds that are set in a modern or futuristic setting feature traffic systems. Virtual reality models can also aid in addressing the challenges of real-world traffic systems, the ever-present gridlock and congestion in cities worldwide: traffic engineers and planners can diagnose system instabilities and explore control strategies in virtual worlds reconstructed from available sensor data. To create these systems, road network models need to be created and represented. Traffic needs to be realistically and efficiently simulated. To analyze real-world scenarios, the traffic conditions need to be estimated and reconstructed. To create virtual scenarios, such as simulated cities, traffic needs to be intelligently and efficiently routed.

  5. Modeling traffic on crossroads

    Science.gov (United States)

    Wastavino, L. A.; Toledo, B. A.; Rogan, J.; Zarama, R.; Muñoz, V.; Valdivia, J. A.

    2007-07-01

    A simplified traffic model is studied, consisting of two vehicles traveling through a sequence of crossroads regulated by yield signs. A car approaching a yield sign stops if, in the other street, there is a car closer than a certain distance xtol from the intersection. It is shown that the function which maps the state of the vehicles displays a period doubling transition to chaos. An interesting feature of the dynamics is that for extremely cautious drivers ( xtol too large), the map turns chaotic, thus becoming a potential source of emergent jams. Complex behavior such as the one observed in this simple system seems to be an essential ingredient in traffic patterns, and could be of relevance in studying actual crossroads situations.

  6. Road Traffic Noise

    Science.gov (United States)

    Beckenbauer, Thomas

    Road traffic is the most interfering noise source in developed countries. According to a publication of the European Union (EU) at the end of the twentieth century [1], about 40% of the population in 15 EU member states is exposed to road traffic noise at mean levels exceeding 55 dB(A). Nearly 80 million people, 20% of the population, are exposed to levels exceeding 65 dB(A) during daytime and more than 30% of the population is exposed to levels exceeding 55 dB(A) during night time. Such high noise levels cause health risks and social disorders (aggressiveness, protest, and helplessness), interference of communication and disturbance of sleep; the long- and short-term consequences cause adverse cardiovascular effects, detrimental hormonal responses (stress hormones), and possible disturbance of the human metabolism (nutrition) and the immune system. Even performance at work and school could be impaired.

  7. Dementia and Traffic Accidents

    DEFF Research Database (Denmark)

    Petersen, Jindong Ding; Siersma, Volkert; Nielsen, Connie Thurøe;

    2016-01-01

    BACKGROUND: As a consequence of a rapid growth of an ageing population, more people with dementia are expected on the roads. Little is known about whether these people are at increased risk of road traffic-related accidents. OBJECTIVE: Our study aims to investigate the risk of road traffic......-related accidents for people aged 65 years or older with a diagnosis of dementia in Denmark. METHODS: We will conduct a nationwide population-based cohort study consisting of Danish people aged 65 or older living in Denmark as of January 1, 2008. The cohort is followed for 7 years (2008-2014). Individual's personal...... data are available in Danish registers and can be linked using a unique personal identification number. A person is identified with dementia if the person meets at least one of the following criteria: (1) a diagnosis of the disease in the Danish National Patient Register or in the Danish Psychiatric...

  8. Yeast Endocytic Adaptor AP-2 Binds the Stress Sensor Mid2 and Functions in Polarized Cell Responses

    Science.gov (United States)

    Chapa-y-Lazo, Bernardo; Allwood, Ellen G; Smaczynska-de Rooij, Iwona I; Snape, Mary L; Ayscough, Kathryn R

    2014-01-01

    The AP-2 complex is a heterotetrameric endocytic cargo-binding adaptor that facilitates uptake of membrane proteins during mammalian clathrin-mediated endocytosis. While budding yeast has clear homologues of all four AP-2 subunits which form a complex and localize to endocytic sites in vivo, the function of yeast AP-2 has remained enigmatic. Here, we demonstrate that AP-2 is required for hyphal growth in Candida albicans and polarized cell responses in Saccharomyces cerevisiae. Deletion of APM4, the cargo-binding mu subunit of AP-2, causes defects in pseudohyphal growth, generation of a mating projection and the cell wall damage response. In an apm4 null mutant, the cell wall stress sensor Mid2 is unable to relocalize to the tip of a mating projection following pheromone addition, or to the mother bud neck in response to cell wall damage. A direct binding interaction between Mid2 and the mu homology domain of Apm4 further supports a model in which AP-2 binds Mid2 to facilitate its internalization and relocalization in response to specific signals. Thus, Mid2 is the first cargo for AP-2 identified in yeast. We propose that endocytic recycling of Mid2 and other components is required for polarized cell responses ensuring cell wall deposition and is tightly monitored during cell growth. PMID:24460703

  9. Untieing the Traffic Knot

    Institute of Scientific and Technical Information of China (English)

    2007-01-01

    Beijing is working to resolve its longtime transportation problems before the 2008 Olympics One traffic jam after another, annoying horns, endless lines of cars,impatient sighs from passengers on an overcrowded bus, the youstomped-on-my-toe quarreling in the subway train, all have led to the unsurprising result that Beijing scored at the tail end of a survey of 287 cities in the category of transport satisfaction in China’s 2006 Report on the Quality of Urban Life.

  10. The Physics of Traffic

    Science.gov (United States)

    Davis, L. Craig

    2006-03-01

    Congestion in freeway traffic is an example of self-organization in the language of complexity theory. Nonequilibrium, first-order phase transitions from free flow cause complex spatiotemporal patterns. Two distinct phases of congestion are observed in empirical traffic data--wide moving jams and synchronous flow. Wide moving jams are characterized by stopped or slowly moving vehicles within the jammed region, which widens and moves upstream at 15-20 km/h. Above a critical density of vehicles, a sudden decrease in the velocity of a lead vehicle can initiate a transition from metastable states to this phase. Human behaviors, especially delayed reactions, are implicated in the formation of jams. The synchronous flow phase results from a bottleneck such as an on-ramp. Thus, in contrast to a jam, the downstream front is pinned at a fixed location. The name of the phase comes from the equilibration (or synchronization) of speed and flow rate across all lanes caused by frequent vehicle lane changes. Synchronous flow occurs when the mainline flow and the rate of merging from an on-ramp are sufficiently large. Large-scale simulations using car-following models reproduce the physical phenomena occurring in traffic and suggest methods to improve flow and mediate congestion.

  11. Effects of traffic noise

    Energy Technology Data Exchange (ETDEWEB)

    Gottlob, D.

    1986-02-01

    One of the main sources of noise is road traffic. In 1984 there were over 25 million cars, 1.2 million lorries, 1.3 million motor cycles and 1.6 million mopeds using our roads. Opinion polls showed that 21% of the population felt that they were affected by traffic noise as a nuisance factor. An outline of the effects of this noise on the affected population is given, illustrated by diagrams. Details about noise emissions (drive-past level) of the different types of vehicles in city traffic are stated and the effects of noise described. The author goes into the nuisance effect (noise is not a physical factor, but a psychosocial one), changes in behaviour (ways of speaking, reduction of stress on households in proportion to rising income and higher educational levels) and the consequences for health (the reaction of the body to noise is primarily a consequence of the psychosomatic organisation of ow bodies). In conclusion, the author deals with the subjective efficiency of noise protection measures. (HWJ).

  12. Assessing Road Traffic Expression

    Directory of Open Access Journals (Sweden)

    Fábio Silva

    2014-12-01

    Full Text Available Road traffic is a problem which is increasing in cities with large population. Unrelated to this fact the number of portable and wearable devices has also been increasing throughout the population of most countries. With this advent, the capacity to monitor and register data about people habits and locations as well as more complex data such as intensity and strength of movements has created an opportunity to contribute to the general wealth and comfort within these environments. Ambient Intelligence and Intelligent Decision Making processes can benefit from the knowledge gathered by these devices to improve decisions on everyday tasks such as deciding navigation routes by car, bicycle or other means of transportation and avoiding route perils. The concept of computational sustainability may also be applied to this problem. Current applications in this area demonstrate the usefulness of real time system that inform the user of certain conditions in the surrounding area. On the other hand, the approach presented in this work aims to describe models and approaches to automatically identify current states of traffic inside cities and use methods from computer science to improve overall comfort and the sustainability of road traffic both with the user and the environment in mind. Such objective is delivered by analyzing real time contributions from those mobile ubiquitous devices to identifying problematic situations and areas under a defined criteria that have significant influence towards a sustainable use of the road transport infrastructure.

  13. Amyloid precursor protein-mediated endocytic pathway disruption induces axonal dysfunction and neurodegeneration.

    Science.gov (United States)

    Xu, Wei; Weissmiller, April M; White, Joseph A; Fang, Fang; Wang, Xinyi; Wu, Yiwen; Pearn, Matthew L; Zhao, Xiaobei; Sawa, Mariko; Chen, Shengdi; Gunawardena, Shermali; Ding, Jianqing; Mobley, William C; Wu, Chengbiao

    2016-05-02

    The endosome/lysosome pathway is disrupted early in the course of both Alzheimer's disease (AD) and Down syndrome (DS); however, it is not clear how dysfunction in this pathway influences the development of these diseases. Herein, we explored the cellular and molecular mechanisms by which endosomal dysfunction contributes to the pathogenesis of AD and DS. We determined that full-length amyloid precursor protein (APP) and its β-C-terminal fragment (β-CTF) act though increased activation of Rab5 to cause enlargement of early endosomes and to disrupt retrograde axonal trafficking of nerve growth factor (NGF) signals. The functional impacts of APP and its various products were investigated in PC12 cells, cultured rat basal forebrain cholinergic neurons (BFCNs), and BFCNs from a mouse model of DS. We found that the full-length wild-type APP (APPWT) and β-CTF both induced endosomal enlargement and disrupted NGF signaling and axonal trafficking. β-CTF alone induced atrophy of BFCNs that was rescued by the dominant-negative Rab5 mutant, Rab5S34N. Moreover, expression of a dominant-negative Rab5 construct markedly reduced APP-induced axonal blockage in Drosophila. Therefore, increased APP and/or β-CTF impact the endocytic pathway to disrupt NGF trafficking and signaling, resulting in trophic deficits in BFCNs. Our data strongly support the emerging concept that dysregulation of Rab5 activity contributes importantly to early pathogenesis of AD and DS.

  14. The endocytic recycling regulator EHD1 is essential for spermatogenesis and male fertility in mice

    Directory of Open Access Journals (Sweden)

    Hess Rex A

    2010-04-01

    Full Text Available Abstract Background The C-terminal Eps15 homology domain-containing protein 1 (EHD1 is ubiquitously expressed and regulates the endocytic trafficking and recycling of membrane components and several transmembrane receptors. To elucidate the function of EHD1 in mammalian development, we generated Ehd1-/- mice using a Cre/loxP system. Results Both male and female Ehd1-/- mice survived at sub-Mendelian ratios. A proportion of Ehd1-/- mice were viable and showed smaller size at birth, which continued into adulthood. Ehd1-/- adult males were infertile and displayed decreased testis size, whereas Ehd1-/- females were fertile. In situ hybridization and immunohistochemistry of developing wildtype mouse testes revealed EHD1 expression in most cells of the seminiferous epithelia. Histopathology revealed abnormal spermatogenesis in the seminiferous tubules and the absence of mature spermatozoa in the epididymides of Ehd1-/- males. Seminiferous tubules showed disruption of the normal spermatogenic cycle with abnormal acrosomal development on round spermatids, clumping of acrosomes, misaligned spermatids and the absence of normal elongated spermatids in Ehd1-/- males. Light and electron microscopy analyses indicated that elongated spermatids were abnormally phagocytosed by Sertoli cells in Ehd1-/- mice. Conclusions Contrary to a previous report, these results demonstrate an important role for EHD1 in pre- and post-natal development with a specific role in spermatogenesis.

  15. Imaging single endocytic events reveals diversity in clathrin, dynamin and vesicle dynamics.

    Science.gov (United States)

    Mattheyses, Alexa L; Atkinson, Claire E; Simon, Sanford M

    2011-10-01

    The dynamics of clathrin-mediated endocytosis can be assayed using fluorescently tagged proteins and total internal reflection fluorescence microscopy. Many of these proteins, including clathrin and dynamin, are soluble and changes in fluorescence intensity can be attributed either to membrane/vesicle movement or to changes in the numbers of individual molecules. It is important for assays to discriminate between physical membrane events and the dynamics of molecules. Two physical events in endocytosis were investigated: vesicle scission from the plasma membrane and vesicle internalization. Single vesicle analysis allowed the characterization of dynamin and clathrin dynamics relative to scission and internalization. We show that vesicles remain proximal to the plasma membrane for variable amounts of time following scission, and that uncoating of clathrin can occur before or after vesicle internalization. The dynamics of dynamin also vary with respect to scission. Results from assays based on physical events suggest that disappearance of fluorescence from the evanescent field should be re-evaluated as an assay for endocytosis. These results illustrate the heterogeneity of behaviors of endocytic vesicles and the importance of establishing suitable evaluation criteria for biophysical processes.

  16. Age-related change of endocytic receptors megalin and cubilin in the kidney in rats.

    Science.gov (United States)

    Odera, Keiko; Goto, Sataro; Takahashi, Ryoya

    2007-10-01

    Megalin and cubilin are the major endocytic receptors responsible for resorption of glomerular filtrate proteins, particularly albumin, in the renal proximal tubule. In order to better understand the mechanism of the development of albuminuria with age in rats, we investigated age-related change of the amount and cellular localization of both receptors in the kidney. Immunoblot analysis of the kidney extracts showed that the amount of megalin significantly decreased with age. Although there was no age-related change in the amount of intact cubilin, the amount of cubilin fragments increased with age. Immunohistochemical study revealed that megalin and cubilin were predominantly localized in brush border membrane of proximal tubular cells in young rats, but the receptors tended to diffuse into the cytoplasm in the old rats. Interestingly, low but significant amounts of megalin and cubilin were present in the glomerular cells in addition to the proximal tubular cells. The quantity of receptors progressively increased in the glomerulus with age. This age-related increase might be to compensate for the age-related defect of the uptake of albumin by the proximal tubules. Thus, although it is unclear whether megalin and cubilin in the glomerulus contribute to the uptake of albumin in primary urine, the age-related increase in the amount of albumin in urine might at least partly be due to quantitative and qualitative alterations of both receptors in the proximal tubule.

  17. Combinatorial SNARE complexes with VAMP7 or VAMP8 define different late endocytic fusion events.

    Science.gov (United States)

    Pryor, Paul R; Mullock, Barbara M; Bright, Nicholas A; Lindsay, Margaret R; Gray, Sally R; Richardson, Simon C W; Stewart, Abigail; James, David E; Piper, Robert C; Luzio, J Paul

    2004-06-01

    Both heterotypic and homotypic fusion events are required to deliver endocytosed macromolecules to lysosomes and remodel late endocytic organelles. A trans-SNARE complex consisting of Q-SNAREs syntaxin 7, Vti1b and syntaxin 8 and the R-SNARE VAMP8 has been shown by others to be responsible for homotypic fusion of late endosomes. Using antibody inhibition experiments in rat liver cell-free systems, we confirmed this result, but found that the same Q-SNAREs can combine with an alternative R-SNARE, namely VAMP7, for heterotypic fusion between late endosomes and lysosomes. Co-immunoprecipitation demonstrated separate syntaxin 7 complexes with either VAMP7 or VAMP8 in solubilized rat liver membranes. Additionally, overexpression of the N-terminal domain of VAMP7, in cultured fibroblastic cells, inhibited the mixing of a preloaded lysosomal content marker with a marker delivered to late endosomes. These data show that combinatorial interactions of SNAREs determine whether late endosomes undergo homotypic or heterotypic fusion events.

  18. Multiple routes of endocytic internalization of PDGFRβ contribute to PDGF-induced STAT3 signaling.

    Science.gov (United States)

    Jastrzębski, Kamil; Zdżalik-Bielecka, Daria; Mamińska, Agnieszka; Kalaidzidis, Yannis; Hellberg, Carina; Miaczynska, Marta

    2017-02-01

    Platelet-derived growth factor receptor β (PDGFRβ) is a receptor tyrosine kinase which upon activation by PDGF-BB stimulates cell proliferation, migration and angiogenesis. Ligand binding induces intracellular signaling cascades but also internalization of the receptor, eventually resulting in its lysosomal degradation. However, endocytic trafficking of receptors often modulates their downstream signaling. We previously reported that internalization of PDGFRβ occurs via dynamin-dependent and -independent pathways but their further molecular determinants remained unknown. Here we show that, in human fibroblasts expressing endogenous PDGFRβ and stimulated with 50 ng/ml PDGF-BB, ligand-receptor uptake proceeds via the parallel routes of clathrin-mediated endocytosis (CME) and clathrin-independent endocytosis (CIE). CME involves the canonical AP2 complex as a clathrin adaptor, while CIE requires RhoA-ROCK, Cdc42 and galectin-3, the latter indicating lectin-mediated internalization via clathrin-independent carriers (CLICs). Although different uptake routes appear to be partly interdependent, they cannot fully substitute for each other. Strikingly, inhibition of any internalization mechanism impaired activation of STAT3 but not of other downstream effectors of PDGFRβ. Our data indicate that multiple routes of internalization of PDGFRβ contribute to a transcriptional and mitogenic response of cells to PDGF.

  19. The polarity protein Par3 regulates APP trafficking and processing through the endocytic adaptor protein Numb.

    Science.gov (United States)

    Sun, Miao; Asghar, Suwaiba Z; Zhang, Huaye

    2016-09-01

    The processing of amyloid precursor protein (APP) into β-amyloid peptide (Aβ) is a key step in the pathogenesis of Alzheimer's disease (AD), and trafficking dysregulations of APP and its secretases contribute significantly to altered APP processing. Here we show that the cell polarity protein Par3 plays an important role in APP processing and trafficking. We found that the expression of full length Par3 is significantly decreased in AD patients. Overexpression of Par3 promotes non-amyloidogenic APP processing, while depletion of Par3 induces intracellular accumulation of Aβ. We further show that Par3 functions by regulating APP trafficking. Loss of Par3 decreases surface expression of APP by targeting APP to the late endosome/lysosome pathway. Finally, we show that the effects of Par3 are mediated through the endocytic adaptor protein Numb, and Par3 functions by interfering with the interaction between Numb and APP. Together, our studies show a novel role for Par3 in regulating APP processing and trafficking.

  20. Caco-2 cell acquisition of dietary iron(III invokes a nanoparticulate endocytic pathway.

    Directory of Open Access Journals (Sweden)

    Dora I A Pereira

    Full Text Available Dietary non-heme iron contains ferrous [Fe(II] and ferric [Fe(III] iron fractions and the latter should hydrolyze, forming Fe(III oxo-hydroxide particles, on passing from the acidic stomach to less acidic duodenum. Using conditions to mimic the in vivo hydrolytic environment we confirmed the formation of nanodisperse fine ferrihydrite-like particles. Synthetic analogues of these (~ 10 nm hydrodynamic diameter were readily adherent to the cell membrane of differentiated Caco-2 cells and internalization was visualized using transmission electron microscopy. Moreover, Caco-2 exposure to these nanoparticles led to ferritin formation (i.e., iron utilization by the cells, which, unlike for soluble forms of iron, was reduced (p=0.02 by inhibition of clathrin-mediated endocytosis. Simulated lysosomal digestion indicated that the nanoparticles are readily dissolved under mildly acidic conditions with the lysosomal ligand, citrate. This was confirmed in cell culture as monensin inhibited Caco-2 utilization of iron from this source in a dose dependent fashion (p<0.05 whilet soluble iron was again unaffected. Our findings reveal the possibility of an endocytic pathway for acquisition of dietary Fe(III by the small intestinal epithelium, which would complement the established DMT-1 pathway for soluble Fe(II.

  1. Endocytic Trafficking of Membrane-Bound Cargo: A Flotillin Point of View

    Directory of Open Access Journals (Sweden)

    Melanie Meister

    2014-07-01

    Full Text Available The ubiquitous and highly conserved flotillin proteins, flotillin-1 and flotillin-2, have been shown to be involved in various cellular processes such as cell adhesion, signal transduction through receptor tyrosine kinases as well as in cellular trafficking pathways. Due to the fact that flotillins are acylated and form hetero-oligomers, they constitutively associate with cholesterol-enriched lipid microdomains. In recent years, such microdomains have been appreciated as platforms that participate in endocytosis and other cellular trafficking steps. This review summarizes the current findings on the role of flotillins in membrane-bound cargo endocytosis and endosomal trafficking events. We will discuss the proposed function of flotillins in endocytosis in the light of recent findings that point towards a role for flotillins in a step that precedes the actual endocytic uptake of cargo molecules. Recent findings have also revealed that flotillins may be important for endosomal sorting and recycling of specific cargo molecules. In addition to these aspects, the cellular trafficking pathway of flotillins themselves as potential cargo in the context of growth factor signaling will be discussed.

  2. Dynamin- and clathrin-dependent endocytic pathway in unicellular eukaryote Paramecium.

    Science.gov (United States)

    Wiejak, Jolanta; Surmacz, Liliana; Wyroba, Elzbieta

    2004-10-01

    The first evidence of dynamin presence and its colocalization with clathrin in the compartment involved in Paramecium receptor-mediated endocytosis is presented. We identified dynamin by cloning, Western blotting, and immunodetection in confocal and electron microscopy. The partial genes, which we have designated ParDyn1 and ParDyn2, are 1091 bp long, 90% identical to one another and encode the N-terminal and middle domains of Paramecium dynamin isoform 1 and isoform 2. The deduced amino acid sequences contain all three guanosine 5'-triphosphate (GTP)-binding motifs and show 67% homology to mammalian dynamins. Antibodies generated against the cloned GTPase domain revealed dynamin association with endosomes containing transferrin, the marker of receptor-mediated endocytosis. In Western blotting a strong immunoreactive polypeptide of approximately 116 kDa, which seems to be phosphorylated, was accompanied by a faint one of approximately 90 kDa in cytosolic fraction (S2). Dynamin level was correlated with internalization of transferrin and it was significantly decreased upon inhibition of this process. Immunogold labeling in electron microscopy revealed colocalization of dynamin and clathrin in coated pits and endocytic vesicles. Moreover, the polypeptide cross-reaction with 2 different antibodies against mammalian clathrin was identified by immunoblotting. These results indicate that dynamin- and clathrin-dependent pathway exists in this evolutionary ancient cell.

  3. Smart Traffic Management Protocol Based on VANET architecture

    Directory of Open Access Journals (Sweden)

    Amilcare Francesco Santamaria

    2014-01-01

    Full Text Available Nowadays one of the hottest theme in wireless environments research is the application of the newest technologies to road safety problems and traffic management exploiting the (VANET architecture. In this work, a novel protocol that aims to achieve a better traffic management is proposed. The overal system is able to reduce traffic level inside the city exploiting inter-communication among vehicles and support infrastructures also known as (V2V and (V2I communications. We design a network protocol called (STMP that takes advantages of IEEE 802.11p standard. On each road several sensors system are placed and they are responsible of monitoring. Gathered data are spread in the network exploiting ad-hoc protocol messages. The increasing knowledge about environment conditions make possible to take preventive actions. Moreover, having a realtime monitoring of the lanes it is possible to reveal roads and city blocks congestions in a shorter time. An external entity to the (VANET is responsible to manage traffic and rearrange traffic along the lanes of the city avoiding huge traffic levels.

  4. Adaptive Resource Allocation and Internet Traffic Engineering on Data Network

    Directory of Open Access Journals (Sweden)

    Hatim Hussein

    2015-02-01

    Full Text Available This research paper describes the issues of bandwid th allocation, optimum capacity allocation, network operational cost reduction, and improve Int ernet user experience. Traffic engineering (TE is used to manipulate network traffic to achie ve certain requirements and meets certain needs. TE becomes one of the most important buildin g blocks in the design of the Internet backbone infrastructure. Research objective: effici ent allocation of bandwidth across multiple paths. Optimum path selection. Minimize network tra ffic delays and maximize bandwidth utilization over multiple network paths. The bandwi dth allocation is performed proportionally over multiple paths based on the path capacity.

  5. Lagrangian Multi-Class Traffic State Estimation

    OpenAIRE

    Yuan, Y.

    2013-01-01

    Road traffic is important to everybody in the world. People travel and commute everyday. For those who travel by cars (or other types of road vehicles), traffic congestion is a daily experience. One essential goal of traffic researchers is to reduce traffic congestion and to improve the whole traffic system operation and the environment. To achieve this goal, we have to first understand prevailing traffic situations, then perform pro-active traffic control and management. The estimation of tr...

  6. Real time traffic models, decision support for traffic management

    OpenAIRE

    Wismans, L.; De Romph, E.; Friso, K.; Zantema, K.

    2014-01-01

    Reliable and accurate short-term traffic state prediction can improve the performance of real-time traffic management systems significantly. Using this short-time prediction based on current measurements delivered by advanced surveillance systems will support decision-making processes on various control strategies and enhance the performance of the overall network. By taking proactive action deploying traffic management measures, congestion may be prevented or its effects limited. An approach...

  7. Traffic Games: Modeling Freeway Traffic with Game Theory.

    Science.gov (United States)

    Cortés-Berrueco, Luis E; Gershenson, Carlos; Stephens, Christopher R

    2016-01-01

    We apply game theory to a vehicular traffic model to study the effect of driver strategies on traffic flow. The resulting model inherits the realistic dynamics achieved by a two-lane traffic model and aims to incorporate phenomena caused by driver-driver interactions. To achieve this goal, a game-theoretic description of driver interaction was developed. This game-theoretic formalization allows one to model different lane-changing behaviors and to keep track of mobility performance. We simulate the evolution of cooperation, traffic flow, and mobility performance for different modeled behaviors. The analysis of these results indicates a mobility optimization process achieved by drivers' interactions.

  8. Traffic Games: Modeling Freeway Traffic with Game Theory

    Science.gov (United States)

    Cortés-Berrueco, Luis E.; Gershenson, Carlos; Stephens, Christopher R.

    2016-01-01

    We apply game theory to a vehicular traffic model to study the effect of driver strategies on traffic flow. The resulting model inherits the realistic dynamics achieved by a two-lane traffic model and aims to incorporate phenomena caused by driver-driver interactions. To achieve this goal, a game-theoretic description of driver interaction was developed. This game-theoretic formalization allows one to model different lane-changing behaviors and to keep track of mobility performance. We simulate the evolution of cooperation, traffic flow, and mobility performance for different modeled behaviors. The analysis of these results indicates a mobility optimization process achieved by drivers’ interactions. PMID:27855176

  9. Framework for Traffic Congestion Management

    OpenAIRE

    Mahmud Hassan TALUKDAR

    2013-01-01

    Traffic Congestion is one of many serious global problems in all great cities resulted from rapid urbanization which always exert negative externalities upon society. The solution of traffic congestion is highly geocentric and due to its heterogeneous nature, curbing congestion is one of the hard tasks for transport planners. It is not possible to suggest unique traffic congestion management framework which could be absolutely applied for every great cities. Conversely, it is quite feasible t...

  10. Traffic forecasts ignoring induced demand

    DEFF Research Database (Denmark)

    Næss, Petter; Nicolaisen, Morten Skou; Strand, Arvid

    2012-01-01

    Although the phenomenon of induced traffic has been theorized for more than 60 years and is now widely accepted among transport researchers, the traffic-generating effects of road capacity expansion are still often neglected in transport modelling. Such omission can lead to serious bias in the as......Although the phenomenon of induced traffic has been theorized for more than 60 years and is now widely accepted among transport researchers, the traffic-generating effects of road capacity expansion are still often neglected in transport modelling. Such omission can lead to serious bias...

  11. Road traffic noise and stroke

    DEFF Research Database (Denmark)

    Sørensen, Mette; Hvidberg, Martin; Andersen, Zorana J

    2011-01-01

    Epidemiological studies suggest that long-term exposure to road traffic noise increases the risk of cardiovascular disorders. The aim of this study was to investigate the relation between exposure to road traffic noise and risk for stroke, which has not been studied before.......Epidemiological studies suggest that long-term exposure to road traffic noise increases the risk of cardiovascular disorders. The aim of this study was to investigate the relation between exposure to road traffic noise and risk for stroke, which has not been studied before....

  12. Road traffic injuries

    Institute of Scientific and Technical Information of China (English)

    WANG Zheng-guo 王正国

    2003-01-01

    @@ The appearance of cars has raised materialistic civilization and living standard to an unprecedented level. Today, it is hard to imagine how we human beings can live without cars.Yet, motor vehicles can cause a great number of deaths and injuries as well as considerable economic losses, which have constituted the global burden. Understanding of the occurrence and development of road traffic injuries will contribute to the prevention and control of crash and to the implementation of "everybody has the right to enjoy health" proposed by WHO.

  13. Inaccuracy in traffic forecasts

    DEFF Research Database (Denmark)

    Flyvbjerg, Bent; Holm, Mette K. Skamris; Buhl, Søren Ladegaard

    2006-01-01

    that forecasters generally do a poor job of estimating the demand for transportation infrastructure projects. The result is substantial downside financial and economic risk. Forecasts have not become more accurate over the 30-year period studied. If techniques and skills for arriving at accurate demand forecasts...... forecasting. Highly inaccurate traffic forecasts combined with large standard deviations translate into large financial and economic risks. But such risks are typically ignored or downplayed by planners and decision-makers, to the detriment of social and economic welfare. The paper presents the data...

  14. Real time traffic models, decision support for traffic management

    NARCIS (Netherlands)

    Wismans, L.J.J.; Romph, de E.; Friso, K.; Zantema, K.

    2014-01-01

    Reliable and accurate short-term traffic state prediction can improve the performance of real-time traffic management systems significantly. Using this short-time prediction based on current measurements delivered by advanced surveillance systems will support decision-making processes on various con

  15. Real Time Traffic Models, Decision Support for Traffic Management

    NARCIS (Netherlands)

    Wismans, L.; De Romph, E.; Friso, K.; Zantema, K.

    2014-01-01

    Reliable and accurate short-term traffic state prediction can improve the performance of real-time traffic management systems significantly. Using this short-time prediction based on current measurements delivered by advanced surveillance systems will support decision-making processes on various con

  16. Traffic Infrastructure in the Development of the Croatian Traffic System

    Directory of Open Access Journals (Sweden)

    Damir Šimulčik

    2012-10-01

    Full Text Available The absence of a long-term traffic policy and of the policyof financing the constntction and maintenance of traffic infrastructurefacilities, represents a synthesis of numerous unresolvedrelations whose negative effects are felt in the overalleconomic and traffic development and consequently theevaluation of national potentials in the field. Adverse aspectcaused by the lack of a clear and feasible policy of financing thetraffic infrastructure facilities, is also a result of not having definedan adequate traffic policy, programme and strategiccourses of development, nor financing models that would be inaccordance with the market and economy system.This indicates that it is necessary to determine a policy forfinancing the constntction and maintenance of traffic infrastntcture,which has to be based on scientific development,team work, availability of plans and programmes to scientistsand experts, determined methodology based on marketing andeconomic logic in defining the programme and strategic tasksand assignments so as to make them feasible.In the near future, intensive preparations for investments inthe overall traffic sysiem are necessary, especially regarding thetraffic infrastntcture facilities - the pivotal points in the processof evaluating the traffic in our national tenitory. Croatia needsto define clearly its strategy in constructing and maintaining thegeneral traffic infrastructure, appointing at the same time thosewho will carry out the given tasks.

  17. Simulation of Traffic on Intersection After Change of Traffic Priority

    Directory of Open Access Journals (Sweden)

    Vladislav KŘIVDA

    2011-06-01

    Full Text Available This paper deals with problem of capacity calculation of intersection after change of traffic priority and with simulation of traffic using simulation software PTV VISSIM. This paper was prepared with financial support for project FRVŠ č. 2206/2011/F1/d “Innovation of Learning of Subjects from the Design of Urban Roads and Intersections”.

  18. Optimal Source-Based Filtering of Malicious Traffic

    CERN Document Server

    Soldo, Fabio; Markopoulou, Athina

    2010-01-01

    In this paper, we consider the problem of blocking malicious traffic on the Internet, via source-based filtering. In particular, we consider filtering via access control lists (ACLs): these are already available at the routers today but are a scarce resource because they are stored in the expensive ternary content addressable memory (TCAM). Aggregation (by filtering source prefixes instead of individual IP addresses) helps reduce the number of filters, but comes also at the cost of blocking legitimate traffic originating from the filtered prefixes. We show how to optimally choose which source prefixes to filter, for a variety of realistic attack scenarios and operators' policies. In each scenario, we design optimal, yet computationally efficient, algorithms. Using logs from Dshield.org, we evaluate the algorithms and demonstrate that they bring significant benefit in practice.

  19. ANALYSIS OF TRAFFIC CONDITIONS IN URBAN THOROUGHFARES

    OpenAIRE

    Kovalenko, L.; Urdzik, S.

    2006-01-01

    Results of experimental investigations into descriptions of traffic flows in urban thoroughfares have been considered. Dependencies between traffic speed, density and intensity for various traffic conditions have been offered.

  20. Probabilistic description of traffic flow

    Science.gov (United States)

    Mahnke, R.; Kaupužs, J.; Lubashevsky, I.

    2005-03-01

    A stochastic description of traffic flow, called probabilistic traffic flow theory, is developed. The general master equation is applied to relatively simple models to describe the formation and dissolution of traffic congestions. Our approach is mainly based on spatially homogeneous systems like periodically closed circular rings without on- and off-ramps. We consider a stochastic one-step process of growth or shrinkage of a car cluster (jam). As generalization we discuss the coexistence of several car clusters of different sizes. The basic problem is to find a physically motivated ansatz for the transition rates of the attachment and detachment of individual cars to a car cluster consistent with the empirical observations in real traffic. The emphasis is put on the analogy with first-order phase transitions and nucleation phenomena in physical systems like supersaturated vapour. The results are summarized in the flux-density relation, the so-called fundamental diagram of traffic flow, and compared with empirical data. Different regimes of traffic flow are discussed: free flow, congested mode as stop-and-go regime, and heavy viscous traffic. The traffic breakdown is studied based on the master equation as well as the Fokker-Planck approximation to calculate mean first passage times or escape rates. Generalizations are developed to allow for on-ramp effects. The calculated flux-density relation and characteristic breakdown times coincide with empirical data measured on highways. Finally, a brief summary of the stochastic cellular automata approach is given.

  1. Reports on internet traffic statistics

    NARCIS (Netherlands)

    Hoogesteger, Martijn; Oliveira Schmidt, de Ricardo; Sperotto, Anna; Pras, Aiko

    2013-01-01

    Internet traffic statistics can provide valuable information to network analysts and researchers about the way nowadays networks are used. In the past, such information was provided by Internet2 in a public website called Internet2 NetFlow: Weekly Reports. The website reported traffic statistics fro

  2. Traffic Light Controller Using Fpga

    Directory of Open Access Journals (Sweden)

    D.Bhavana

    2015-04-01

    Full Text Available The traffic light sequence works on the specific switching of Red, Green and Yellow lights in a particular way with stipulated time form. The normal function of traffic lights requires sophisticated control and coordination to ensure that traffic moves as smoothly and safely as possible and that pedestrians are protected when they cross the roads [1] .This Traffic Light sequence is generated using a specific switching mechanism which will help to control a traffic light system on a road in a specified sequence. This paper focuses on the fact that the traffic lights can be varied in the day and night mode depending on the intensity of the traffic. It plays a vital role in supervising and running the metropolitan traffic and evade the possibilities of any unfortunate mishaps happening in and around the cities. It is a sequential machine to be scrutinized as per the requirements and programmed through a multistep development process. The methods that are used in this project are proposing the circuit, write a code, simulate, synthesis and implement on the hardware [8] . In this project, XILINX Software was chosen to devise a schematic using schematic edit, write a code using Verilog HDL (Hardware Description Language text editor and implements the circuit on Programmable Logic Device [PLD].The system has been successfully tested and implemented in hardware using Nexys 2 Digilent FPGA.

  3. CD36 is not involved in scavenger receptor-mediated endocytic uptake of glycolaldehyde- and methylglyoxal-modified proteins by liver endothelial cells.

    Science.gov (United States)

    Nakajou, Keisuke; Horiuchi, Seikoh; Sakai, Masakazu; Hirata, Kenshiro; Tanaka, Makiko; Takeya, Motohiro; Kai, Toshiya; Otagiri, Masaki

    2005-05-01

    Circulating proteins modified by advanced glycation end-products (AGE) are mainly taken up by liver endothelial cells (LECs) via scavenger receptor-mediated endocytosis. Endocytic uptake of chemically modified proteins by macrophages and macrophage-derived cells is mediated by class A scavenger receptor (SR-A) and CD36. In a previous study using SR-A knockout mice, we demonstrated that SR-A is not involved in endocytic uptake of AGE proteins by LECs [Matsumoto et al. (2000) Biochem. J. 352, 233-240]. The present study was conducted to determine the contribution of CD36 to this process. Glycolaldehyde-modified BSA (GA-BSA) and methylglyoxal-modified BSA (MG-BSA) were used as AGE proteins. 125I-GA-BSA and 125I-MG-BSA underwent endocytic degradation by these cells at 37 degrees C, and this process was inhibited by several ligands for the scavenger receptors. However, this endocytic uptake of 125I-GA-BSA by LECs was not inhibited by a neutralizing anti-CD36 antibody. Similarly, hepatic uptake of (111)In-GA-BSA after its intravenous injection was not significantly attenuated by co-administration of the anti-CD36 antibody. These results clarify that CD36 does not play a significant role in elimination of GA-BSA and MG-BSA from the circulation, suggesting that the receptor involved in endocytic uptake of circulating AGE proteins by LEC is not SR-A or CD36.

  4. Endocytic pathway rapidly delivers internalized molecules to lysosomes: an analysis of vesicle trafficking, clustering and mass transfer.

    Science.gov (United States)

    Pangarkar, Chinmay; Dinh, Anh-Tuan; Mitragotri, Samir

    2012-08-20

    Lysosomes play a critical role in intracellular drug delivery. For enzyme-based therapies, they represent a potential target site whereas for nucleic acid or many protein drugs, they represent the potential degradation site. Either way, understanding the mechanisms and processes involved in routing of materials to lysosomes after cellular entry is of high interest to the field of drug delivery. Most therapeutic cargoes other than small hydrophobic molecules enter the cells through endocytosis. Endocytosed cargoes are routed to lysosomes via microtubule-based transport and are ultimately shared by various lysosomes via tethering and clustering of endocytic vesicles followed by exchange of their contents. Using a combined experimental and numerical approach, here we studied the rates of mass transfer into and among the endocytic vesicles in a model cell line, 3T3 fibroblasts. In order to understand the relationship of mass transfer with microtubular transport and vesicle clustering, we varied both properties through various pharmacological agents. At the same time, microtubular transport and vesicle clustering were modeled through diffusion-advection equations and the Smoluchowski equations, respectively. Our analysis revealed that the rate of mass transfer is optimally related to microtubular transport and clustering properties of vesicles. Further, the rate of mass transfer is highest in the innate state of the cell. Any perturbation to either microtubular transport or vesicle aggregation led to reduced mass transfer to lysosome. These results suggest that in the absence of an external intervention the endocytic pathway appears to maximize molecular delivery to lysosomes. Strategies are discussed to reduce mass transfer to lysosomes so as to extend the residence time of molecules in endosomes or late endosomes, thus potentially increasing the likelihood of their escape before disposition in the lysosomes.

  5. Web traffic and firm performance

    DEFF Research Database (Denmark)

    Farooq, Omar; Aguenaou, Samir

    2013-01-01

    into better firm performance? This paper aims to answer these questions by documenting a positive relationship between the extent of web-traffic and firm performance in the MENA region during the 2010. We argue that higher web-traffic lowers the agency problems in firms by disseminating more information...... to stock market participants. Consequently, lower agency problems translate into better performance. Furthermore, we also show that agency reducing role of web-traffic is more pronounced in regimes where information environment is already bad. For example, our results show stronger impact of web......-traffic on firm performance in civil law countries, firms with concentrated ownership, and firms with more intangible assets. All of these groups are characterized by higher agency problems. Our results, therefore, indicate that web-traffic can play a substitute for traditional governance mechanisms in the MENA...

  6. Expression and immunolocalisation of the endocytic receptors megalin and cubilin in the human yolk sac and placenta across gestation.

    Science.gov (United States)

    Burke, K A; Jauniaux, E; Burton, G J; Cindrova-Davies, T

    2013-11-01

    Megalin and cubilin are multifunctional endocytic receptors associated with many transporting epithelia. They play an essential role in transport of nutrients through the visceral yolk sac of rodents during embryogenesis. Here, we immunolocalise them to the endodermal layer of the human yolk sac, and to the syncytiotrophoblast and cytotrophoblast cells of placental villi. In villi, the protein level of both receptors increased with gestation. The mRNA for megalin remained constant, while that encoding cubilin increased with gestation. These results suggest megalin and cubilin may be important in human maternal-fetal transfer, and that they increase across gestation to facilitate this function.

  7. Regulation of endocytic trafficking of transferrin receptor by optineurin and its impairment by a glaucoma-associated mutant

    Directory of Open Access Journals (Sweden)

    Rangaraj Nandini

    2010-01-01

    Full Text Available Abstract Background Optineurin is a multifunctional protein involved in several functions such as vesicular trafficking from the Golgi to the plasma membrane, NF-κB regulation, signal transduction and gene expression. Mutations in optineurin are associated with glaucoma, a neurodegenerative eye disease that causes blindness. Genetic evidence suggests that the E50K (Glu50Lys is a dominant disease-causing mutation of optineurin. However, functional alterations caused by mutations in optineurin are not known. Here, we have analyzed the role of optineurin in endocytic recycling and the effect of E50K mutant on this process. Results We show that the knockdown of optineurin impairs trafficking of transferrin receptor to the juxtanuclear region. A point mutation (D474N in the ubiquitin-binding domain abrogates localization of optineurin to the recycling endosomes and interaction with transferrin receptor. The function of ubiquitin-binding domain of optineurin is also needed for trafficking of transferrin to the juxtanuclear region. A disease causing mutation, E50K, impairs endocytic recycling of transferrin receptor as shown by enlarged recycling endosomes, slower dynamics of E50K vesicles and decreased transferrin uptake by the E50K-expressing cells. This impaired trafficking by the E50K mutant requires the function of its ubiquitin-binding domain. Compared to wild type optineurin, the E50K optineurin shows enhanced interaction and colocalization with transferrin receptor and Rab8. The velocity of Rab8 vesicles is reduced by co-expression of the E50K mutant. These results suggest that the E50K mutant affects Rab8-mediated transferrin receptor trafficking. Conclusions Our results suggest that optineurin regulates endocytic trafficking of transferrin receptor to the juxtanuclear region. The E50K mutant impairs trafficking at the recycling endosomes due to altered interactions with Rab8 and transferrin receptor. These results also have implications for

  8. Traffic information computing platform for big data

    Energy Technology Data Exchange (ETDEWEB)

    Duan, Zongtao, E-mail: ztduan@chd.edu.cn; Li, Ying, E-mail: ztduan@chd.edu.cn; Zheng, Xibin, E-mail: ztduan@chd.edu.cn; Liu, Yan, E-mail: ztduan@chd.edu.cn; Dai, Jiting, E-mail: ztduan@chd.edu.cn; Kang, Jun, E-mail: ztduan@chd.edu.cn [Chang' an University School of Information Engineering, Xi' an, China and Shaanxi Engineering and Technical Research Center for Road and Traffic Detection, Xi' an (China)

    2014-10-06

    Big data environment create data conditions for improving the quality of traffic information service. The target of this article is to construct a traffic information computing platform for big data environment. Through in-depth analysis the connotation and technology characteristics of big data and traffic information service, a distributed traffic atomic information computing platform architecture is proposed. Under the big data environment, this type of traffic atomic information computing architecture helps to guarantee the traffic safety and efficient operation, more intelligent and personalized traffic information service can be used for the traffic information users.

  9. A Framework for Detection of Traffic Anomalies Based on IP Aggregation

    Science.gov (United States)

    Zhanikeev, Marat; Tanaka, Yoshiaki

    Traditional traffic analysis is can be performed online only when detection targets are well specified and are fairly primitive. Local processing at measurement point is discouraged as it would considerably affect major functionality of a network device. When traffic is analyzed at flow level, the notion of flow timeout generates differences in flow lifespan and impedes unbiased monitoring, where only n-top flows ordered by a certain metric are considered. This paper proposes an alternative manner of traffic analysis based on source IP aggregation. The method uses flows as basic building blocks but ignores timeouts, using short monitoring intervals instead. Multidimensional space of metrics obtained through IP aggregation, however, enhances capabilities of traffic analysis by facilitating detection of various anomalous conditions in traffic simultaneously.

  10. Probing heterobivalent binding to the endocytic AP-2 adaptor complex by DNA-based spatial screening.

    Science.gov (United States)

    Diezmann, F; von Kleist, L; Haucke, V; Seitz, O

    2015-08-01

    The double helical DNA scaffold offers a unique set of properties, which are particularly useful for studies of multivalency in biomolecular interactions: (i) multivalent ligand displays can be formed upon nucleic acid hybridization in a self-assembly process, which facilitates spatial screening (ii) valency and spatial arrangement of the ligand display can be precisely controlled and (iii) the flexibility of the ligand display can be adjusted by integrating nick sites and unpaired template regions. Herein we describe the use of DNA-based spatial screening for the characterization of the adaptor complex 2 (AP-2), a central interaction hub within the endocytic protein network in clathrin-mediated endocytosis. AP-2 is comprised of a core domain and two, so-called appendage domains, the α- and the β2-ear, which associate with cytoplasmatic proteins required for the formation or maturation of clathrin/AP-2 coated pits. Each appendage domain has two binding grooves which recognize distinct peptide motives with micromolar affinity. This provides opportunities for enhanced interactions with protein molecules that contain two (or more) different peptide motives. To determine whether a particular, spatial arrangement of binding motifs is required for high affinity binding we probed the distance-affinity relationships by means of DNA-programmed spatial screening with self-assembled peptide-DNA complexes. By using trimolecular and tetramolecular assemblies two different peptides were positioned in 2-22 nucleotide distance. The binding data obtained with both recombinant protein in well-defined buffer systems and native AP-2 in brain extract suggests that the two binding sites of the AP-2 α-appendage can cooperate to provide up to 40-fold enhancement of affinity compared to the monovalent interaction. The distance between the two recognized peptide motives was less important provided that the DNA duplex segments were connected by flexible, single strand segments. By

  11. Statistical Multiplexing of Video Traffic and Data Traffic

    Institute of Scientific and Technical Information of China (English)

    2005-01-01

    This paper studies statistical multiplexing performance byinput of video traffic and data traffic. The inputs have different Qos requirements such as loss and delay jitter. By applying a modified FBM model, we present methods to estimate effective bandwidth of the aggregated traffic. Simulationswere paformed to evaluate effective bandwidth. The comparison between the estimation and the simulation shows that the estimations can give correct data for the effective bandwidths in terms of our interests. The analysis of gain by using priority multiplexing also addresses proper Qos configuration for the inputs in order to achieve positive gains.

  12. Endocytic trafficking from the small intestinal brush border probed with FM dye

    DEFF Research Database (Denmark)

    Hansen, Gert H; Rasmussen, Karina; Niels-Christiansen, Lise-Lotte;

    2009-01-01

    The small intestinal brush border functions as the body's main portal for uptake of dietary nutrients and simultaneously acts as the largest permeability barrier against pathogens. To enable this, the digestive enzymes of the brush border are organized in lipid raft microdomains stabilized by cross...... localized deeper into the cytoplasm of the enterocytes. Two major raft-associated brush border enzymes, alkaline phosphatase and aminopeptidase N, were excluded from endocytosis. We propose that the terminal web cytoskeleton, by inhibiting traffic from apical early endosomes further into the cell......, contributes to the overall permeability barrier of the gut. Key words: FM dye, small intestine, brush border, endocytosis....

  13. Ultrasound guided supraclavicular block.

    LENUS (Irish Health Repository)

    Hanumanthaiah, Deepak

    2013-09-01

    Ultrasound guided regional anaesthesia is becoming increasingly popular. The supraclavicular block has been transformed by ultrasound guidance into a potentially safe superficial block. We reviewed the techniques of performing supraclavicular block with special focus on ultrasound guidance.

  14. Framework for Traffic Congestion Management

    Directory of Open Access Journals (Sweden)

    Mahmud Hassan TALUKDAR

    2013-06-01

    Full Text Available Traffic Congestion is one of many serious global problems in all great cities resulted from rapid urbanization which always exert negative externalities upon society. The solution of traffic congestion is highly geocentric and due to its heterogeneous nature, curbing congestion is one of the hard tasks for transport planners. It is not possible to suggest unique traffic congestion management framework which could be absolutely applied for every great cities. Conversely, it is quite feasible to develop a framework which could be used with or without minor adjustment to deal with congestion problem. So, the main aim of this paper is to prepare a traffic congestion mitigation framework which will be useful for urban planners, transport planners, civil engineers, transport policy makers, congestion management researchers who are directly or indirectly involved or willing to involve in the task of traffic congestion management. Literature review is the main source of information of this study. In this paper, firstly, traffic congestion is defined on the theoretical point of view and then the causes of traffic congestion are briefly described. After describing the causes, common management measures, using world- wide, are described and framework for supply side and demand side congestion management measures are prepared.

  15. Road traffic injuries in Mozambique.

    Science.gov (United States)

    Romão, Francelina; Nizamo, Hanifa; Mapasse, Domingos; Rafico, Momede Mussá; José, João; Mataruca, Simão; Efron, M Lúcia; Omondi, Lucas O; Leifert, Thelma; Bicho, Joaquim M L Marungo

    2003-01-01

    Road traffic injuries affect the economy, health and quality of life of the people of Mozambique. Current road safety programmes are inadequate and inefficient given the magnitude of the problem. Data reported on road traffic crashes in the period 1990 to 2000 from the National Institute for Road Safety, the traffic police and the Central Hospital of Maputo were reviewed. The burden of road traffic injuries in Mozambique is rising, with at least three people killed daily. The age group most affected is 25-38 (39.35%), followed by 16-24 (20.79%). The main causes of crashes include reckless driving, drunken driving, roads with potholes, inadequate signs, lack of protection for pedestrians, and inadequate traffic law enforcement. However, the data are not adequate to reveal the true magnitude of the problem. Data collected by different sources are incomplete and not coordinated with other sources and databases. In urban areas, however, better response to crashes, treatment of the injured, reporting and data collection is attributable to a greater concentration of police and medical facilities. Road traffic safety programmes in Mozambique are inadequate and inefficient, starting with the data collection system. Improvement of injury surveillance systems is needed to help make road traffic safety a national development agenda priority and for developing and implementing road safety policies. For road safety programmes to be effective, government must facilitate stakeholders' involvement, and the clear definition of government activities, civil society activities and public-private partnerships need to be established.

  16. Slit-Dependent Endocytic Trafficking of the Robo Receptor Is Required for Son of Sevenless Recruitment and Midline Axon Repulsion.

    Science.gov (United States)

    Chance, Rebecca K; Bashaw, Greg J

    2015-09-01

    Understanding how axon guidance receptors are activated by their extracellular ligands to regulate growth cone motility is critical to learning how proper wiring is established during development. Roundabout (Robo) is one such guidance receptor that mediates repulsion from its ligand Slit in both invertebrates and vertebrates. Here we show that endocytic trafficking of the Robo receptor in response to Slit-binding is necessary for its repulsive signaling output. Dose-dependent genetic interactions and in vitro Robo activation assays support a role for Clathrin-dependent endocytosis, and entry into both the early and late endosomes as positive regulators of Slit-Robo signaling. We identify two conserved motifs in Robo's cytoplasmic domain that are required for its Clathrin-dependent endocytosis and activation in vitro; gain of function and genetic rescue experiments provide strong evidence that these trafficking events are required for Robo repulsive guidance activity in vivo. Our data support a model in which Robo's ligand-dependent internalization from the cell surface to the late endosome is essential for receptor activation and proper repulsive guidance at the midline by allowing recruitment of the downstream effector Son of Sevenless in a spatially constrained endocytic trafficking compartment.

  17. Endocytic Ark/Prk kinases play a critical role in adriamycin resistance in both yeast and mammalian cells.

    Science.gov (United States)

    Takahashi, Tsutomu; Furuchi, Takemitsu; Naganuma, Akira

    2006-12-15

    To elucidate the mechanism of acquired resistance to Adriamycin, we searched for genes that, when overexpressed, render Saccharomyces cerevisiae resistant to Adriamycin. We identified AKL1, a gene of which the function is unknown but is considered, nonetheless, to be a member of the Ark/Prk kinase family, which is involved in the regulation of endocytosis, on the basis of its deduced amino acid sequence. Among tested members of the Ark/Prk kinase family (Ark1, Prk1, and Akl1), overexpressed Prk1 also conferred Adriamycin resistance on yeast cells. Prk1 is known to dissociate the Sla1/Pan1/End3 complex, which is involved in endocytosis, by phosphorylating Sla1 and Pan1 in the complex. We showed that Akl1 promotes phosphorylation of Pan1 in this complex and reduces the endocytic ability of the cell, as does Prk1. Sla1- and End3-defective yeast cells were also resistant to Adriamycin and overexpression of Akl1 in these defective cells did not increase the degree of Adriamycin resistance, suggesting that Akl1 might reduce Adriamycin toxicity by reducing the endocytic ability of cells via a mechanism that involves the Sla1/Pan1/End3 complex and the phosphorylation of Pan1. We also found that HEK293 cells that overexpressed AAK1, a member of the human Ark/Prk family, were Adriamycin resistant. Our findings suggest that endocytosis might be involved in the mechanism of Adriamycin toxicity in yeast and human cells.

  18. Regulation of the V-ATPase along the endocytic pathway occurs through reversible subunit association and membrane localization.

    Directory of Open Access Journals (Sweden)

    Céline Lafourcade

    Full Text Available The lumen of endosomal organelles becomes increasingly acidic when going from the cell surface to lysosomes. Luminal pH thereby regulates important processes such as the release of internalized ligands from their receptor or the activation of lysosomal enzymes. The main player in endosomal acidification is the vacuolar ATPase (V-ATPase, a multi-subunit transmembrane complex that pumps protons from the cytoplasm to the lumen of organelles, or to the outside of the cell. The active V-ATPase is composed of two multi-subunit domains, the transmembrane V(0 and the cytoplasmic V(1. Here we found that the ratio of membrane associated V(1/Vo varies along the endocytic pathway, the relative abundance of V(1 being higher on late endosomes than on early endosomes, providing an explanation for the higher acidity of late endosomes. We also found that all membrane-bound V-ATPase subunits were associated with detergent resistant membranes (DRM isolated from late endosomes, raising the possibility that association with lipid-raft like domains also plays a role in regulating the activity of the proton pump. In support of this, we found that treatment of cells with U18666A, a drug that leads to the accumulation of cholesterol in late endosomes, affected acidification of late endosome. Altogether our findings indicate that the activity of the vATPase in the endocytic pathway is regulated both by reversible association/dissociation and the interaction with specific lipid environments.

  19. Generation of stable lipid raft microdomains in the enterocyte brush border by selective endocytic removal of non-raft membrane.

    Science.gov (United States)

    Danielsen, E Michael; Hansen, Gert H

    2013-01-01

    The small intestinal brush border has an unusually high proportion of glycolipids which promote the formation of lipid raft microdomains, stabilized by various cross-linking lectins. This unique membrane organization acts to provide physical and chemical stability to the membrane that faces multiple deleterious agents present in the gut lumen, such as bile salts, digestive enzymes of the pancreas, and a plethora of pathogens. In the present work, we studied the constitutive endocytosis from the brush border of cultured jejunal explants of the pig, and the results indicate that this process functions to enrich the contents of lipid raft components in the brush border. The lipophilic fluorescent marker FM, taken up into early endosomes in the terminal web region (TWEEs), was absent from detergent resistant membranes (DRMs), implying an association with non-raft membrane. Furthermore, neither major lipid raft-associated brush border enzymes nor glycolipids were detected by immunofluorescence microscopy in subapical punctae resembling TWEEs. Finally, two model raft lipids, BODIPY-lactosylceramide and BODIPY-GM1, were not endocytosed except when cholera toxin subunit B (CTB) was present. In conclusion, we propose that constitutive, selective endocytic removal of non-raft membrane acts as a sorting mechanism to enrich the brush border contents of lipid raft components, such as glycolipids and the major digestive enzymes. This sorting may be energetically driven by changes in membrane curvature when molecules move from a microvillar surface to an endocytic invagination.

  20. Generation of stable lipid raft microdomains in the enterocyte brush border by selective endocytic removal of non-raft membrane.

    Directory of Open Access Journals (Sweden)

    E Michael Danielsen

    Full Text Available The small intestinal brush border has an unusually high proportion of glycolipids which promote the formation of lipid raft microdomains, stabilized by various cross-linking lectins. This unique membrane organization acts to provide physical and chemical stability to the membrane that faces multiple deleterious agents present in the gut lumen, such as bile salts, digestive enzymes of the pancreas, and a plethora of pathogens. In the present work, we studied the constitutive endocytosis from the brush border of cultured jejunal explants of the pig, and the results indicate that this process functions to enrich the contents of lipid raft components in the brush border. The lipophilic fluorescent marker FM, taken up into early endosomes in the terminal web region (TWEEs, was absent from detergent resistant membranes (DRMs, implying an association with non-raft membrane. Furthermore, neither major lipid raft-associated brush border enzymes nor glycolipids were detected by immunofluorescence microscopy in subapical punctae resembling TWEEs. Finally, two model raft lipids, BODIPY-lactosylceramide and BODIPY-GM1, were not endocytosed except when cholera toxin subunit B (CTB was present. In conclusion, we propose that constitutive, selective endocytic removal of non-raft membrane acts as a sorting mechanism to enrich the brush border contents of lipid raft components, such as glycolipids and the major digestive enzymes. This sorting may be energetically driven by changes in membrane curvature when molecules move from a microvillar surface to an endocytic invagination.

  1. Slit-Dependent Endocytic Trafficking of the Robo Receptor Is Required for Son of Sevenless Recruitment and Midline Axon Repulsion.

    Directory of Open Access Journals (Sweden)

    Rebecca K Chance

    2015-09-01

    Full Text Available Understanding how axon guidance receptors are activated by their extracellular ligands to regulate growth cone motility is critical to learning how proper wiring is established during development. Roundabout (Robo is one such guidance receptor that mediates repulsion from its ligand Slit in both invertebrates and vertebrates. Here we show that endocytic trafficking of the Robo receptor in response to Slit-binding is necessary for its repulsive signaling output. Dose-dependent genetic interactions and in vitro Robo activation assays support a role for Clathrin-dependent endocytosis, and entry into both the early and late endosomes as positive regulators of Slit-Robo signaling. We identify two conserved motifs in Robo's cytoplasmic domain that are required for its Clathrin-dependent endocytosis and activation in vitro; gain of function and genetic rescue experiments provide strong evidence that these trafficking events are required for Robo repulsive guidance activity in vivo. Our data support a model in which Robo's ligand-dependent internalization from the cell surface to the late endosome is essential for receptor activation and proper repulsive guidance at the midline by allowing recruitment of the downstream effector Son of Sevenless in a spatially constrained endocytic trafficking compartment.

  2. Traffic jams: dynamics and control.

    Science.gov (United States)

    Orosz, Gábor; Wilson, R Eddie; Stépán, Gábor

    2010-10-13

    This introductory paper reviews the current state-of-the-art scientific methods used for modelling, analysing and controlling the dynamics of vehicular traffic. Possible mechanisms underlying traffic jam formation and propagation are presented from a dynamical viewpoint. Stable and unstable motions are described that may give the skeleton of traffic dynamics, and the effects of driver behaviour are emphasized in determining the emergent state in a vehicular system. At appropriate points, references are provided to the papers published in the corresponding Theme Issue.

  3. OPTIMASI BANDWITH MENGGUNAKAN TRAFFIC SHAPPING

    Directory of Open Access Journals (Sweden)

    Imam Riadi

    2012-05-01

    Full Text Available Perkembangan layanan komunikasi telah berkembang sangat pesat. Salah satunya adalah pemanfaatan penggunaan bandwith untuk mengakases jaringan Internet. Traffic shapping bandwidth dapat memberikan efesiensi dalam hal pemanfaatan bandwidth pada instansi yang melakukan manajemen dalam lalulintas jaringannya. Metode yang digunakan dalam penelitian ini adalah metode literatur yaitu metode pengumpulan data yang dilakukan dengan mengumpulkan sumber-sumber data yang terkait dan metode eksperimen yaitu melakukan penelitian dengan mengkonfigurasi Mikrotik RouterOS untuk melakukan traffic shapping bandwidth. Hasil penelitian ini adalah router yang dapat melakukan traffic shapping sehingga kualitas koneksi menjadi lebih baik untuk mengakses dan mengambil data dari sebuah website.

  4. Traffic Light Detection at Night

    DEFF Research Database (Denmark)

    Jensen, Morten Bornø; Philipsen, Mark Philip; Bahnsen, Chris

    2015-01-01

    Traffic light recognition (TLR) is an integral part of any in- telligent vehicle, it must function both at day and at night. However, the majority of TLR research is focused on day-time scenarios. In this paper we will focus on detection of traffic lights at night and evalu- ate the performance...... of three detectors based on heuristic models and one learning-based detector. Evaluation is done on night-time data from the public LISA Traffic Light Dataset. The learning-based detector out- performs the model-based detectors in both precision and recall. The learning-based detector achieves an average...

  5. [Comics for traffic education: evaluation of a traffic safety campaign].

    Science.gov (United States)

    Bonfadelli, H

    1989-01-01

    Traffic safety campaigns often are ineffective to change driving behavior because they don't reach the target group or are recognized only by people who are already interested or concerned. The evaluation of a traffic safety campaign called "Leo Lässig", addressed to young new drivers, shows that recognition and acceptance by the target group were stimulated by the age-conform means of comic-strips.

  6. Characteristics of synchronized traffic in mixed traffic flow

    Institute of Scientific and Technical Information of China (English)

    Ning Hong-Xin; Xue Yu

    2012-01-01

    In this paper,the characteristics of synchronized traffic in mixed traffic flow are investigated based on the braking light model.By introducing the energy dissipation and the distribution of slowdown vehicles,the effects of the maximum velocity,the mixing ratio,and the length of vehicles on the synchronized flow are discussed.It is found that the maximum velocity plays a great role in the synchronized flow in mixed traffic.The energy dissipation and the distribution of slowdown vehicles in the synchronized flow region are greatly different from those in free flow and a traffic jamming region.When all of vehicles have the same maximum velocity with Vmax > 15,the mixed traffic significantly displays synchronized flow,which has been demonstrated by the relation between flow rate and occupancy and estimation of the cross-correlation function.Moreover,the energy dissipation in the synchronized flow region does not increase with occupancy.The distribution of slowdown vehicles shows a changeless platform in the synchronized flow region.This is an interesting phenomenon. It helps to deeply understand the synchronized flow and greatly reduce the energy dissipation of traffic flow.

  7. Block Cipher Analysis

    DEFF Research Database (Denmark)

    Miolane, Charlotte Vikkelsø

    Block ciphersarecryptographicprimitivesthatoperateon fixed sizetexts(blocks). Mostdesigns aim towards secure andfastencryption oflarge amounts ofdata. Block ciphers also serve as the building block of a number of hash functions and message authentication codes(MAC).Thetask of cryptanalysisisto en...... on small scale variants of AES. In the final part of the thesis we present a new block cipher proposal Present and examine its security against algebraic and differential cryptanalysis in particular.......Block ciphersarecryptographicprimitivesthatoperateon fixed sizetexts(blocks). Mostdesigns aim towards secure andfastencryption oflarge amounts ofdata. Block ciphers also serve as the building block of a number of hash functions and message authentication codes(MAC).Thetask of cryptanalysisisto...... ensurethat no attack violatesthe securitybounds specifiedbygeneric attack namely exhaustivekey search and table lookup attacks. This thesis contains a general introduction to cryptography with focus on block ciphers and important block cipher designs, in particular the Advanced Encryption Standard...

  8. Lagrangian Multi-Class Traffic State Estimation

    NARCIS (Netherlands)

    Yuan, Y.

    2013-01-01

    Road traffic is important to everybody in the world. People travel and commute everyday. For those who travel by cars (or other types of road vehicles), traffic congestion is a daily experience. One essential goal of traffic researchers is to reduce traffic congestion and to improve the whole traffi

  9. Study on Macro-traffic Model

    Institute of Scientific and Technical Information of China (English)

    FangGe; GuowuZhang; BaojunYuan; KepingLi

    2004-01-01

    Macro-traffic model is an effective tool for supporting traffic programmer to This article is based on the software products VISEM (demand model) and VISUM (supply model) from PTV Company of Germany, and studies macro-traffic model on the basis of considering that traffic system is an interactive system of between a supply system and a demand system.

  10. Assessment of Traffic Noise Impacts

    DEFF Research Database (Denmark)

    Rich, Jeppe Husted; Nielsen, Otto Anker

    2004-01-01

    A steady growth in traffic intensities in most urban areas throughout the world has forced planners and politicians to seriously consider the resulting environmental impact, such as traffic noise, accidents and air pollution. The assessment of such negative factors is needed in order to reveal...... the true social benefit of infrastructure plans. The paper presents a noise assessment model for the Copenhagen region, which brings together GIS technology and non-linear hedonic regression models to reveal the implicit costs of traffic noise measured as the marginal percentage loss in property values...... with respect to the decibel traffic noise. The model distinguishes between houses and apartments and shows that the ability to include refined accessibility variables have significant impact on estimated prices....

  11. Intelligent Ambulance Traffic Assistance System

    Directory of Open Access Journals (Sweden)

    RONOJOY GHOSH

    2013-09-01

    Full Text Available With the increase in traffic road density, several causalities occur due to delay in taking a patient to the hospital in an ambulance. In this paper, we have developed an algorithm to find the shortest path to reach the required destination. As required the software will identify the present location of the vehicle and ask the user for the destination. Then it will show all the available paths, highlighting the shortest one or in several cases the most optimum one. Further we made the traffic signals automated for special vehicles like an ambulance or a fire-engine such that the signals will go green for the ambulance as it comes in the vicinity of the traffic signal, thus providing them with a clear path to reach its destination. The original signal is restored as soon as the ambulance goes undetected by the Bluetooth scanner of the traffic signal.

  12. A multiagent urban traffic simulation

    CERN Document Server

    Tranouez, Pierrick; Langlois, Patrice

    2012-01-01

    We built a multiagent simulation of urban traffic to model both ordinary traffic and emergency or crisis mode traffic. This simulation first builds a modeled road network based on detailed geographical information. On this network, the simulation creates two populations of agents: the Transporters and the Mobiles. Transporters embody the roads themselves; they are utilitarian and meant to handle the low level realism of the simulation. Mobile agents embody the vehicles that circulate on the network. They have one or several destinations they try to reach using initially their beliefs of the structure of the network (length of the edges, speed limits, number of lanes etc.). Nonetheless, when confronted to a dynamic, emergent prone environment (other vehicles, unexpectedly closed ways or lanes, traffic jams etc.), the rather reactive agent will activate more cognitive modules to adapt its beliefs, desires and intentions. It may change its destination(s), change the tactics used to reach the destination (favorin...

  13. Automatic Traffic Recorder (ATR) Stations

    Data.gov (United States)

    Department of Homeland Security — The data included in the GIS Traffic Stations Version database have been assimilated from station description files provided by FHWA for Weigh-in-Motion (WIM), and...

  14. Estimating Emissions from Railway Traffic

    DEFF Research Database (Denmark)

    Jørgensen, Morten W.; Sorenson, Spencer C.

    1998-01-01

    Several parameters of importance for estimating emissions from railway traffic are discussed, and typical results presented. Typical emissions factors from diesel engines and electrical power generation are presented, and the effect of differences in national electrical generation sources...

  15. AAA Foundation for Traffic Safety

    Science.gov (United States)

    ... kind of road user. 1 2 3 4 Traffic Safety News Distracted driving fines are up, but ... detailed data regarding when, where, and how much people drive and goes beyond existing federal data. Research ...

  16. Urothelial endocytic vesicle recycling and lysosomal degradative pathway regulated by lipid membrane composition.

    Science.gov (United States)

    Grasso, E J; Calderón, R O

    2013-02-01

    The urothelium, a specialized epithelium that covers the mucosa cell surface of the urinary bladder, undergoes dramatic morphological changes during the micturition cycle that involve a membrane apical traffic. This traffic was first described as a lysosomal pathway, in addition to the known endocytosis/exocytosis membrane recycling. In an attempt to understand the role of membrane lipid composition in those effects, we previously described the lipid-dependent leakage of the endocytosed vesicle content. In this work, we demonstrated clear differences in the traffic of both the fluid probe and the membrane-bound probe in urothelial umbrella cells by using spectrofluorometry and/or confocal and epifluorescence microscopy. Different membrane lipid compositions were established by using three diet formulae enriched in oleic acid, linoleic acid and a commercial formula. Between three and five animals for each dietary treatment were used for each analysis. The decreased endocytosis of both fluid and membrane-bound probes (approximately 32 and 49 % lower, respectively) in oleic acid-derived umbrella cells was concomitant with an increased recycling (approximately 4.0 and 3.7 times, respectively) and diminished sorting to the lysosome (approximately 23 and 37 %, respectively) when compared with the control umbrella cells. The higher intravesicular pH and the impairment of the lysosomal pathway of oleic acid diet-derived vesicles compared to linoleic acid diet-derived vesicles and control diet-derived vesicles correlate with our findings of a lower V-ATPase activity previously reported. We integrated the results obtained in the present and previous work to determine the sorting of endocytosed material (fluid and membrane-bound probes) into the different cell compartments. Finally, the weighted average effect of the individual alterations on the intracellular distribution was evaluated. The results shown in this work add evidences for the modulatory role of the membrane

  17. A Survey on Urban Traffic Management System Using Wireless Sensor Networks

    Directory of Open Access Journals (Sweden)

    Kapileswar Nellore

    2016-01-01

    Full Text Available Nowadays, the number of vehicles has increased exponentially, but the bedrock capacities of roads and transportation systems have not developed in an equivalent way to efficiently cope with the number of vehicles traveling on them. Due to this, road jamming and traffic correlated pollution have increased with the associated adverse societal and financial effect on different markets worldwide. A static control system may block emergency vehicles due to traffic jams. Wireless Sensor networks (WSNs have gained increasing attention in traffic detection and avoiding road congestion. WSNs are very trendy due to their faster transfer of information, easy installation, less maintenance, compactness and for being less expensive compared to other network options. There has been significant research on Traffic Management Systems using WSNs to avoid congestion, ensure priority for emergency vehicles and cut the Average Waiting Time (AWT of vehicles at intersections. In recent decades, researchers have started to monitor real-time traffic using WSNs, RFIDs, ZigBee, VANETs, Bluetooth devices, cameras and infrared signals. This paper presents a survey of current urban traffic management schemes for priority-based signalling, and reducing congestion and the AWT of vehicles. The main objective of this survey is to provide a taxonomy of different traffic management schemes used for avoiding congestion. Existing urban traffic management schemes for the avoidance of congestion and providing priority to emergency vehicles are considered and set the foundation for further research.

  18. A Survey on Urban Traffic Management System Using Wireless Sensor Networks.

    Science.gov (United States)

    Nellore, Kapileswar; Hancke, Gerhard P

    2016-01-27

    Nowadays, the number of vehicles has increased exponentially, but the bedrock capacities of roads and transportation systems have not developed in an equivalent way to efficiently cope with the number of vehicles traveling on them. Due to this, road jamming and traffic correlated pollution have increased with the associated adverse societal and financial effect on different markets worldwide. A static control system may block emergency vehicles due to traffic jams. Wireless Sensor networks (WSNs) have gained increasing attention in traffic detection and avoiding road congestion. WSNs are very trendy due to their faster transfer of information, easy installation, less maintenance, compactness and for being less expensive compared to other network options. There has been significant research on Traffic Management Systems using WSNs to avoid congestion, ensure priority for emergency vehicles and cut the Average Waiting Time (AWT) of vehicles at intersections. In recent decades, researchers have started to monitor real-time traffic using WSNs, RFIDs, ZigBee, VANETs, Bluetooth devices, cameras and infrared signals. This paper presents a survey of current urban traffic management schemes for priority-based signalling, and reducing congestion and the AWT of vehicles. The main objective of this survey is to provide a taxonomy of different traffic management schemes used for avoiding congestion. Existing urban traffic management schemes for the avoidance of congestion and providing priority to emergency vehicles are considered and set the foundation for further research.

  19. An Open-Source Microscopic Traffic Simulator

    CERN Document Server

    Treiber, Martin; 10.1109/MITS.2010.939208

    2010-01-01

    We present the interactive Java-based open-source traffic simulator available at www.traffic-simulation.de. In contrast to most closed-source commercial simulators, the focus is on investigating fundamental issues of traffic dynamics rather than simulating specific road networks. This includes testing theories for the spatiotemporal evolution of traffic jams, comparing and testing different microscopic traffic models, modeling the effects of driving styles and traffic rules on the efficiency and stability of traffic flow, and investigating novel ITS technologies such as adaptive cruise control, inter-vehicle and vehicle-infrastructure communication.

  20. Stochastic control of traffic patterns

    DEFF Research Database (Denmark)

    Gaididei, Yuri B.; Gorria, Carlos; Berkemer, Rainer

    2013-01-01

    A stochastic modulation of the safety distance can reduce traffic jams. It is found that the effect of random modulation on congestive flow formation depends on the spatial correlation of the noise. Jam creation is suppressed for highly correlated noise. The results demonstrate the advantage...... of heterogeneous performance of the drivers in time as well as individually. This opens the possibility for the construction of technical tools to control traffic jam formation....

  1. TrafficGen Architecture Document

    Science.gov (United States)

    2016-01-01

    Poisson, Jitter, and Clone . Researchers can experience an added dimension of network traffic visualization by pairing TrafficGen with the NRL Scripted...MVC Classes The top-level MVC classes control the workspace of the application, specifically the menu and the way the nodes and events are rendered...This is a container class that holds singleton instances of the view classes employed by this application. Because of the way this application is

  2. Future Emissions from Railway Traffic

    DEFF Research Database (Denmark)

    Sorenson, Spencer C.

    1998-01-01

    In investigation of the expected development in factors which influence railway energy consumption and emissions. Traffic factors such as train speed, load, an occupancy were considered. Tehcnical factors such an emissions factors, fleet composition and train weight were also considered. An estim......In investigation of the expected development in factors which influence railway energy consumption and emissions. Traffic factors such as train speed, load, an occupancy were considered. Tehcnical factors such an emissions factors, fleet composition and train weight were also considered...

  3. Internet Load and Traffic Balancing

    Institute of Scientific and Technical Information of China (English)

    李文正; 郭巧; 郭为民

    2005-01-01

    Load and traffic balancing for the Internet are analyzed. An intelligent domain name system(DNS) policy for the control and management of content distribution networks (CDN) is presented. An CDNs network based Urtitech IntelliDNS is built, and performance of the network in realizing traffic and load balancing is measured. It is proved that CDNs provide a valuable service and the methods are feasible.

  4. Estimating emissions from railway traffic

    Energy Technology Data Exchange (ETDEWEB)

    Joergensen, M.W.; Sorenson, C.

    1997-07-01

    The report discusses methods that can be used to estimate the emissions from various kinds of railway traffic. The methods are based on the estimation of the energy consumption of the train, so that comparisons can be made between electric and diesel driven trains. Typical values are given for the necessary traffic parameters, emission factors, and train loading. Detailed models for train energy consumption are presented, as well as empirically based methods using average train speed and distance between stop. (au)

  5. Fastlane: Traffic flow modeling and multi-class dynamic traffic management

    NARCIS (Netherlands)

    Schreiter, T.; Van Wageningen-Kessels, F.L.M.; Yuan, Y.; Van Lint, J.W.C.; Hoogendoorn, S.P.

    2012-01-01

    Dynamic Traffic Management (DTM) aims to improve traffic conditions. DTM usually consists of two steps: first the current traffic is estimated, then appropriate control actions are determined based on that estimate. In order to estimate and control the traffic, a suitable traffic flow model that rep

  6. Classification of Automated Search Traffic

    Science.gov (United States)

    Buehrer, Greg; Stokes, Jack W.; Chellapilla, Kumar; Platt, John C.

    As web search providers seek to improve both relevance and response times, they are challenged by the ever-increasing tax of automated search query traffic. Third party systems interact with search engines for a variety of reasons, such as monitoring a web site’s rank, augmenting online games, or possibly to maliciously alter click-through rates. In this paper, we investigate automated traffic (sometimes referred to as bot traffic) in the query stream of a large search engine provider. We define automated traffic as any search query not generated by a human in real time. We first provide examples of different categories of query logs generated by automated means. We then develop many different features that distinguish between queries generated by people searching for information, and those generated by automated processes. We categorize these features into two classes, either an interpretation of the physical model of human interactions, or as behavioral patterns of automated interactions. Using the these detection features, we next classify the query stream using multiple binary classifiers. In addition, a multiclass classifier is then developed to identify subclasses of both normal and automated traffic. An active learning algorithm is used to suggest which user sessions to label to improve the accuracy of the multiclass classifier, while also seeking to discover new classes of automated traffic. Performance analysis are then provided. Finally, the multiclass classifier is used to predict the subclass distribution for the search query stream.

  7. Rab, Arf, and Arl-Regulated Membrane Traffic in Cortical Neuron Migration.

    Science.gov (United States)

    Tang, Bor Luen

    2016-07-01

    The migration of projection neurons from its birthplace in the subventricular zone to their final destination in the cortical plate is a complex process that requires a series of highly coordinated cellular events. Amongst the key factors involved in the processes are modulators of cytoskeletal dynamics, as well as cellular membrane traffic. Members of the small GTPases family responsible for the latter process, the Rabs and Arfs, have been recently implicated in cortical neuron migration. Rab5 and Rab11, which are key modulators of endocytosis and endocytic recycling respectively, ensure proper surface expression and distribution of N-cadherin, a key adhesion protein that tethers migrating neurons to the radial glia fiber tracts during pia-directed migration. Rab7, which is associated with lysosomal biogenesis and function, is important for the final step of terminal translocation when N-cadherin is downregulated by lysosomal degradation. Arf6 activity, which is known to be important in neuronal processes outgrowth, may negatively impact the multipolar-bipolar transition of cortical neurons undergoing radial migration, but the downstream effector of Arf6 in this regard is not yet known. In addition to the above, members of the Arl family which have been recently shown to be important in radial glia scaffold formation, would also be important for cortical neuron migration. In this short review, we discuss recent advances in our understanding of the importance of membrane traffic regulated by the Rab, Arf, and Arl family members in cortical neuron migration.

  8. Reciprocal expression of the endocytic protein HIP1R and its repressor FOXP1 predicts outcome in R-CHOP-treated diffuse large B-cell lymphoma patients

    DEFF Research Database (Denmark)

    Wong, K K; Gascoyne, D M; Brown, P J

    2014-01-01

    We previously identified autoantibodies to the endocytic-associated protein Huntingtin-interacting protein 1-related (HIP1R) in diffuse large B-cell lymphoma (DLBCL) patients. HIP1R regulates internalization of cell surface receptors via endocytosis, a process relevant to many therapeutic strateg...

  9. TRAFFIC INCIDENT MANAGEMENT IN THE PRESENCE OF HAZARDS

    Directory of Open Access Journals (Sweden)

    Z. Wang

    2016-09-01

    Full Text Available Traffic incidents can result in different kinds of hazards (e.g., plumes that influence the status of road networks, therefore there is a great need for incident management in the presence of the hazards. When incidents occur, the created hazards not only affect the normal road users (make them detour or blocked, but also influence the movement of first responders. Traffic managers, who are responsible for maintaining the road safety and traffic stability, should carry out quick and effective measures to manage the incidents. In this paper, we present four issues to help people better understand the situations that could occur in the management of incidents with hazards: 1. Evacuation in the presence of hazards; 2. 3D incident management; 3. Navigation support for first responders; 4. Navigation support for road users. To address these issues, we propose a solution which combines agent technology, geo-database, hazard simulation, and traffic simulation. Further research would be needed to investigate the potentials of the proposed solution in real applications.

  10. Traffic Incident Management in the Presence of Hazards

    Science.gov (United States)

    Wang, Z.; Zlatanova, S.; Steenbruggen, J.

    2016-09-01

    Traffic incidents can result in different kinds of hazards (e.g., plumes) that influence the status of road networks, therefore there is a great need for incident management in the presence of the hazards. When incidents occur, the created hazards not only affect the normal road users (make them detour or blocked), but also influence the movement of first responders. Traffic managers, who are responsible for maintaining the road safety and traffic stability, should carry out quick and effective measures to manage the incidents. In this paper, we present four issues to help people better understand the situations that could occur in the management of incidents with hazards: 1). Evacuation in the presence of hazards; 2). 3D incident management; 3). Navigation support for first responders; 4). Navigation support for road users. To address these issues, we propose a solution which combines agent technology, geo-database, hazard simulation, and traffic simulation. Further research would be needed to investigate the potentials of the proposed solution in real applications.

  11. Generation of stable lipid raft microdomains in the enterocyte brush border by selective endocytic removal of non-raft membrane

    DEFF Research Database (Denmark)

    Danielsen, E Michael; Hansen, Gert H

    2013-01-01

    multiple deleterious agents present in the gut lumen, such as bile salts, digestive enzymes of the pancreas, and a plethora of pathogens. In the present work, we studied the constitutive endocytosis from the brush border of cultured jejunal explants of the pig, and the results indicate that this process...... major lipid raft-associated brush border enzymes nor glycolipids were detected by immunofluorescence microscopy in subapical punctae resembling TWEEs. Finally, two model raft lipids, BODIPY-lactosylceramide and BODIPY-GM1, were not endocytosed except when cholera toxin subunit B (CTB) was present....... In conclusion, we propose that constitutive, selective endocytic removal of non-raft membrane acts as a sorting mechanism to enrich the brush border contents of lipid raft components, such as glycolipids and the major digestive enzymes. This sorting may be energetically driven by changes in membrane curvature...

  12. Differential actions of the endocytic collagen receptor uPARAP/Endo180 and the collagenase MMP-2 in bone homeostasis

    DEFF Research Database (Denmark)

    Madsen, Daniel H; Jürgensen, Henrik J; Ingvarsen, Signe;

    2013-01-01

    the extracellular collagenase, MMP-2, and the endocytic collagen receptor, uPARAP, by generating mice with combined deficiency of both components. In both uPARAP-deficient and MMP-2-deficient adult mice the length of the tibia and femur was decreased, along with a reduced bone mineral density and trabecular bone...... quality. An additional decrease in bone length was observed when combining the two deficiencies, pointing to both components being important for the remodeling processes in long bone growth. In agreement with results found by others, a different effect of MMP-2 deficiency was observed in the distinct bone...... structures of the calvaria. These membranous bones were found to be thickened in MMP-2-deficient mice, an effect likely to be related to an accompanying defect in the canalicular system. Surprisingly, both of the latter defects in MMP-2-deficient mice were counteracted by concurrent uPARAP deficiency...

  13. Synchronized flow in oversaturated city traffic.

    Science.gov (United States)

    Kerner, Boris S; Klenov, Sergey L; Hermanns, Gerhard; Hemmerle, Peter; Rehborn, Hubert; Schreckenberg, Michael

    2013-11-01

    Based on numerical simulations with a stochastic three-phase traffic flow model, we reveal that moving queues (moving jams) in oversaturated city traffic dissolve at some distance upstream of the traffic signal while transforming into synchronized flow. It is found that, as in highway traffic [Kerner, Phys. Rev. E 85, 036110 (2012)], such a jam-absorption effect in city traffic is explained by a strong driver's speed adaptation: Time headways (space gaps) between vehicles increase upstream of a moving queue (moving jam), resulting in moving queue dissolution. It turns out that at given traffic signal parameters, the stronger the speed adaptation effect, the shorter the mean distance between the signal location and the road location at which moving queues dissolve fully and oversaturated traffic consists of synchronized flow only. A comparison of the synchronized flow in city traffic found in this Brief Report with synchronized flow in highway traffic is made.

  14. Percolation properties in a traffic model

    CERN Document Server

    Wang, Feilong; Xu, Xiaoyun; Wu, Ruoqian; Havlin, Shlomo

    2015-01-01

    As a dynamical complex system, traffic is characterized by a transition from free flow to congestions, which is mostly studied in highways. However, despite its importance in developing congestion mitigation strategies, the understanding of this common traffic phenomenon in a city-scale is still missing. An open question is how the traffic in the network collapses from a global efficient traffic to isolated local flows in small clusters, i.e. the question of traffic percolation. Here we study the traffic percolation properties on a lattice by simulation of an agent-based model for traffic. A critical traffic volume in this model distinguishes the free-state from congested state of traffic. Our results show that the threshold of traffic percolation decreases with increasing traffic volume and reaches a minimum value at the critical traffic volume. We show that this minimal threshold is the result of longest spatial correlation between traffic flows at the critical traffic volume. These findings may help to dev...

  15. Interaction of the Human Papillomavirus E6 Oncoprotein with Sorting Nexin 27 Modulates Endocytic Cargo Transport Pathways.

    Directory of Open Access Journals (Sweden)

    Ketaki Ganti

    2016-09-01

    Full Text Available A subset of high-risk Human Papillomaviruses (HPVs are the causative agents of a large number of human cancers, of which cervical is the most common. Two viral oncoproteins, E6 and E7, contribute directly towards the development and maintenance of malignancy. A characteristic feature of the E6 oncoproteins from cancer-causing HPV types is the presence of a PDZ binding motif (PBM at its C-terminus, which confers interaction with cellular proteins harbouring PDZ domains. Here we show that this motif allows E6 interaction with Sorting Nexin 27 (SNX27, an essential component of endosomal recycling pathways. This interaction is highly conserved across E6 proteins from multiple high-risk HPV types and is mediated by a classical PBM-PDZ interaction but unlike many E6 targets, SNX27 is not targeted for degradation by E6. Rather, in HPV-18 positive cell lines the association of SNX27 with components of the retromer complex and the endocytic transport machinery is altered in an E6 PBM-dependent manner. Analysis of a SNX27 cargo, the glucose transporter GLUT1, reveals an E6-dependent maintenance of GLUT1 expression and alteration in its association with components of the endocytic transport machinery. Furthermore, knockdown of E6 in HPV-18 positive cervical cancer cells phenocopies the loss of SNX27, both in terms of GLUT1 expression levels and its vesicular localization, with a concomitant marked reduction in glucose uptake, whilst loss of SNX27 results in slower cell proliferation in low nutrient conditions. These results demonstrate that E6 interaction with SNX27 can alter the recycling of cargo molecules, one consequence of which is modulation of nutrient availability in HPV transformed tumour cells.

  16. Traffic Signal Using Smart Agent System

    Directory of Open Access Journals (Sweden)

    Cheonshik Kim

    2008-01-01

    Full Text Available In this research, we propose an electro-sensitive traffic light using the smart agent algorithm to reduce traffic congestion and traffic accidents. The multi-agent system approach can provide a new and preferable solution. The proposed method adaptively controls the cycle of traffic signals even though the traffic volume varies. Consequently, we reduce the car waiting time and start-up delay time using fuzzy control of feedback data. In particular, we have designed and implemented a system to create optimum traffic signals in congested conditions. The effectiveness of this method was shown through simulation of multiple intersections.

  17. Structure Model of Urban Traffic System Evolution

    Institute of Scientific and Technical Information of China (English)

    JIANG Ke-jin; ZHANG Dian-ye

    2008-01-01

    A structure model of urban traffic system evolution is built based on the analysis of the factors influencing the system evolution and the hierarchy between the factors. Then the influencing degrees of the factors are quantificationally analyzed by DEMATE (decision making trial and evaluation laboratory). The analysis results indicate that the traffic mode structure which achieves the highest central degree is the dominant influencing factor of the urban traffic system evolution, and that economy development and the traffic poficy axe the second important factors that also affect the traffic mode structures. Furthermore, physical geography is a basic restriction to the urban traffic system evolution.

  18. Simulation Model of Traffic Jam at Crossroads

    Directory of Open Access Journals (Sweden)

    Mladen Kalajžić

    2002-11-01

    Full Text Available Traffic congestion is one of the major problems in most cities.It is the consequence of unavoidable motorization, butalso, in many cases, of improper solutions considering constructionof roads or organisation of traffic.This paper deals with one problematic crossroad in thetown of Zadar in which traffic jams occur due to poor organisationof traffic. Using mathematical simulation, the first partproves that traffic jams will certainly occur, and in the secondpart, crossroads signalling is considered as a possible solutionwhich, if combined with intelligent control could significantlyimprove the organisation of traffic at this crossroads.

  19. Hidden geometry of traffic jamming

    Science.gov (United States)

    Andjelković, Miroslav; Gupte, Neelima; Tadić, Bosiljka

    2015-05-01

    We introduce an approach based on algebraic topological methods that allow an accurate characterization of jamming in dynamical systems with queues. As a prototype system, we analyze the traffic of information packets with navigation and queuing at nodes on a network substrate in distinct dynamical regimes. A temporal sequence of traffic density fluctuations is mapped onto a mathematical graph in which each vertex denotes one dynamical state of the system. The coupling complexity between these states is revealed by classifying agglomerates of high-dimensional cliques that are intermingled at different topological levels and quantified by a set of geometrical and entropy measures. The free-flow, jamming, and congested traffic regimes result in graphs of different structure, while the largest geometrical complexity and minimum entropy mark the edge of the jamming region.

  20. On vehicular traffic data analysis

    Energy Technology Data Exchange (ETDEWEB)

    Brics, Martins; Mahnke, Reinhard [Institute of Physics, Rostock University (Germany)

    2011-07-01

    This contribution consists of analysis of empirical vehicular traffic flow data. The main focus lies on the Next Generation Simulation (NGSIM) data. The first findings show that there are artificial structures within the data due to errors of monitoring as well as smoothing position measurement data. As a result speed data show discretisation in 5 feet per second. The aim of this investigation is to construct microscopic traffic flow models which are in agreement to the analysed empirical data. The ongoing work follows the subject of research summarized by Christof Liebe in his PhD thesis entitled ''Physics of traffic flow: Empirical data and dynamical models'' (Rostock, 2010).

  1. Symmetry breaking in optimal timing of traffic signals on an idealized two-way street

    Science.gov (United States)

    Panaggio, Mark J.; Ottino-Löffler, Bertand J.; Hu, Peiguang; Abrams, Daniel M.

    2013-09-01

    Simple physical models based on fluid mechanics have long been used to understand the flow of vehicular traffic on freeways; analytically tractable models of flow on an urban grid, however, have not been as extensively explored. In an ideal world, traffic signals would be timed such that consecutive lights turned green just as vehicles arrived, eliminating the need to stop at each block. Unfortunately, this “green-wave” scenario is generally unworkable due to frustration imposed by competing demands of traffic moving in different directions. Until now this has typically been resolved by numerical simulation and optimization. Here, we develop a theory for the flow in an idealized system consisting of a long two-way road with periodic intersections. We show that optimal signal timing can be understood analytically and that there are counterintuitive asymmetric solutions to this signal coordination problem. We further explore how these theoretical solutions degrade as traffic conditions vary and automotive density increases.

  2. Symmetry breaking in optimal timing of traffic signals on an idealized two-way street

    CERN Document Server

    Panaggio, Mark J; Hu, Peiguang; Abrams, Daniel M

    2013-01-01

    Simple physical models based on fluid mechanics have long been used to understand the flow of vehicular traffic on freeways; analytically tractable models of flow on an urban grid, however, have not been as extensively explored. In an ideal world, traffic signals would be timed such that consecutive lights turned green just as vehicles arrived, eliminating the need to stop at each block. Unfortunately, this "green wave" scenario is generally unworkable due to frustration imposed by competing demands of traffic moving in different directions. Until now this has typically been resolved by numerical simulation and optimization. Here, we develop a theory for the flow in an idealized system consisting of a long two-way road with periodic intersections. We show that optimal signal timing can be understood analytically and that there are counter-intuitive asymmetric solutions to this signal coordination problem. We further explore how these theoretical solutions degrade as traffic conditions vary and automotive dens...

  3. Microtubule-based transport -basic mechanisms, traffic rules and role in neurological pathogenesis

    NARCIS (Netherlands)

    M.A.M. Franker (Mariella); C.C. Hoogenraad (Casper)

    2013-01-01

    textabstractMicrotubule-based transport is essential for neuronal function because of the large distances that must be traveled by various building blocks and cellular materials. Recent studies in various model systems have unraveled several regulatory mechanisms and traffic rules that control the s

  4. Traffic and Granular Flow '11

    CERN Document Server

    Buslaev, Alexander; Bugaev, Alexander; Yashina, Marina; Schadschneider, Andreas; Schreckenberg, Michael; TGF11

    2013-01-01

    This book continues the biannual series of conference proceedings, which has become a classical reference resource in traffic and granular research alike. It addresses new developments at the interface between physics, engineering and computational science. Complex systems, where many simple agents, be they vehicles or particles, give rise to surprising and fascinating phenomena.   The contributions collected in these proceedings cover several research fields, all of which deal with transport. Topics include highway, pedestrian and internet traffic, granular matter, biological transport, transport networks, data acquisition, data analysis and technological applications. Different perspectives, i.e. modeling, simulations, experiments and phenomenological observations, are considered.

  5. Traffic-Sign Recognition Systems

    CERN Document Server

    Escalera, Sergio

    2011-01-01

    This work presents a full generic approach to the detection and recognition of traffic signs. The approach is based on the latest computer vision methods for object detection, and on powerful methods for multiclass classification. The challenge was to robustly detect a set of different sign classes in real time, and to classify each detected sign into a large, extensible set of classes. To address this challenge, several state-of-the-art methods were developed that can be used for different recognition problems. Following an introduction to the problems of traffic sign detection and categoriza

  6. Agent Based Individual Traffic Guidance

    DEFF Research Database (Denmark)

    Wanscher, Jørgen

    This thesis investigates the possibilities in applying Operations Research (OR) to autonomous vehicular traffic. The explicit difference to most other research today is that we presume that an agent is present in every vehicle - hence Agent Based Individual Traffic guidance (ABIT). The next...... evolutionary step for the in-vehicle route planners is the introduction of two-way communication. We presume that the agent is capable of exactly this. Based on this presumption we discuss the possibilities and define a taxonomy and use this to discuss the ABIT system. Based on a set of scenarios we conclude...

  7. Traffic dynamics around weaving section influenced by accident: Cellular automata approach

    Science.gov (United States)

    Kong, Lin-Peng; Li, Xin-Gang; Lam, William H. K.

    2015-07-01

    The weaving section, as a typical bottleneck, is one source of vehicle conflicts and an accident-prone area. Traffic accident will block lanes and the road capacity will be reduced. Several models have been established to study the dynamics around traffic bottlenecks. However, little attention has been paid to study the complex traffic dynamics influenced by the combined effects of bottleneck and accident. This paper presents a cellular automaton model to characterize accident-induced traffic behavior around the weaving section. Some effective control measures are proposed and verified for traffic management under accident condition. The total flux as a function of inflow rates, the phase diagrams, the spatial-temporal diagrams, and the density and velocity profiles are presented to analyze the impact of accident. It was shown that the proposed control measures for weaving traffic can improve the capacity of weaving section under both normal and accident conditions; the accidents occurring on median lane in the weaving section are more inclined to cause traffic jam and reduce road capacity; the capacity of weaving section will be greatly reduced when the accident happens downstream the weaving section.

  8. Asymptotic Analysis of a Loss Model with Trunk Reservation I: Trunks Reserved for Fast Traffic

    Directory of Open Access Journals (Sweden)

    John A. Morrison

    2008-01-01

    Full Text Available We consider a model for a single link in a circuit-switched network. The link has C circuits, and the input consists of offered calls of two types, that we call primary and secondary traffic. Of the C links, R are reserved for primary traffic. We assume that both traffic types arrive as Poisson arrival streams. Assuming that C is large and R=O(1, the arrival rate of primary traffic is O(C, while that of secondary traffic is smaller, of the order O(C. The holding times of the primary calls are assumed to be exponentially distributed with unit mean. Those of the secondary calls are exponentially distributed with a large mean, that is, O(C. Thus, the primary calls have fast arrivals and fast service, compared to the secondary calls. The loads for both traffic types are comparable (O(C, and we assume that the system is “critically loaded”; that is, the system's capacity is approximately equal to the total load. We analyze asymptotically the steady state probability that n1 (resp., n2 circuits are occupied by primary (resp., secondary calls. In particular, we obtain two-term asymptotic approximations to the blocking probabilities for both traffic types.

  9. Robust and Adaptive Block Tracking Method Based on Particle Filter

    Directory of Open Access Journals (Sweden)

    Bin Sun

    2015-10-01

    Full Text Available In the field of video analysis and processing, object tracking is attracting more and more attention especially in traffic management, digital surveillance and so on. However problems such as objects’ abrupt motion, occlusion and complex target structures would bring difficulties to academic study and engineering application. In this paper, a fragmentsbased tracking method using the block relationship coefficient is proposed. In this method, we use particle filter algorithm and object region is divided into blocks initially. The contribution of this method is that object features are not extracted just from a single block, the relationship between current block and its neighbor blocks are extracted to describe the variation of the block. Each block is weighted according to the block relationship coefficient when the block is voted on the most matched region in next frame. This method can make full use of the relationship between blocks. The experimental results demonstrate that our method can provide good performance in condition of occlusion and abrupt posture variation.

  10. Total Spinal Block after Thoracic Paravertebral Block.

    Science.gov (United States)

    Beyaz, Serbülent Gökhan; Özocak, Hande; Ergönenç, Tolga; Erdem, Ali Fuat; Palabıyık, Onur

    2014-02-01

    Thoracic paravertebral block (TPVB) can be performed with or without general anaesthesia for various surgical procedures. TPVB is a popular anaesthetic technique due to its low side effect profile and high analgesic potency. We used 20 mL of 0.5% levobupivacaine for a single injection of unilateral TPVB at the T7 level with neurostimulator in a 63 year old patient with co-morbid disease who underwent cholecystectomy. Following the application patient lost consciousness, and was intubated. Haemodynamic instability was normalised with rapid volume replacement and vasopressors. Anaesthetic drugs were stopped at the end of the surgery and muscle relaxant was antagonised. Return of mucle strenght was shown with neuromuscular block monitoring. Approximately three hours after TPVB, spontaneous breathing started and consciousness returned. A total spinal block is a rare and life-threatening complication. A total spinal block is a complication of spinal anaesthesia, and it can also occur after peripheral blocks. Clinical presentation is characterised by hypotension, bradicardia, apnea, and cardiac arrest. An early diagnosis and appropriate treatment is life saving. In this case report, we want to present total spinal block after TPVB.

  11. Fuzzy Multiobjective Traffic Light Signal Optimization

    Directory of Open Access Journals (Sweden)

    N. Shahsavari Pour

    2013-01-01

    Full Text Available Traffic congestion is a major concern for many cities throughout the world. In a general traffic light controller, the traffic lights change at a constant cycle time. Hence it does not provide an optimal solution. Many traffic light controllers in current use are based on the “time-of-the-day” scheme, which use a limited number of predetermined traffic light patterns and implement these patterns depending upon the time of the day. These automated systems do not provide an optimal control for fluctuating traffic volumes. In this paper, the fuzzy traffic light controller is used to optimize the control of fluctuating traffic volumes such as oversaturated or unusual load conditions. The problem is solved by genetic algorithm, and a new defuzzification method is introduced. The performance of the new defuzzification method (NDM is compared with the centroid point defuzzification method (CPDM by using ANOVA. Finally, an illustrative example is presented to show the competency of proposed algorithm.

  12. Wireless traffic steering for green cellular networks

    CERN Document Server

    Zhang, Shan; Zhou, Sheng; Niu, Zhisheng; Shen, Xuemin (Sherman)

    2016-01-01

    This book introduces wireless traffic steering as a paradigm to realize green communication in multi-tier heterogeneous cellular networks. By matching network resources and dynamic mobile traffic demand, traffic steering helps to reduce on-grid power consumption with on-demand services provided. This book reviews existing solutions from the perspectives of energy consumption reduction and renewable energy harvesting. Specifically, it explains how traffic steering can improve energy efficiency through intelligent traffic-resource matching. Several promising traffic steering approaches for dynamic network planning and renewable energy demand-supply balancing are discussed. This book presents an energy-aware traffic steering method for networks with energy harvesting, which optimizes the traffic allocated to each cell based on the renewable energy status. Renewable energy demand-supply balancing is a key factor in energy dynamics, aimed at enhancing renewable energy sustainability to reduce on-grid energy consum...

  13. Highway and traffic engineering in developing countries

    DEFF Research Database (Denmark)

    1996-01-01

    Describes road and traffic engineering methods and problems in developing countries as opposed to similar problems in industrialized countries......Describes road and traffic engineering methods and problems in developing countries as opposed to similar problems in industrialized countries...

  14. Generalized Block Failure

    DEFF Research Database (Denmark)

    Jönsson, Jeppe

    2015-01-01

    Block tearing is considered in several codes as a pure block tension or a pure block shear failure mechanism. However in many situations the load acts eccentrically and involves the transfer of a substantial moment in combination with the shear force and perhaps a normal force. A literature study...... yield lines around the block leads to simple interaction formulas similar to other interaction formulas in the codes.......Block tearing is considered in several codes as a pure block tension or a pure block shear failure mechanism. However in many situations the load acts eccentrically and involves the transfer of a substantial moment in combination with the shear force and perhaps a normal force. A literature study...

  15. Poisson vs. Long-Tailed Internet traffic

    OpenAIRE

    2005-01-01

    In this thesis, we reexamine the long discussion on which model is suitable for studying Internet traffic: Poisson or Long-tailed Internet traffic. Poisson model, adapted from telephone network, has been used since the beginning of World Wide Web, while long-tailed distribution gradually takes over with believable evidence. Instead of using Superposition of Point Processes to explain why traffic that is not Poisson tends towards Poisson traffic as the load increases, as it is recent...

  16. Vehicle Traffic Congestion Estimation Based on RFID

    OpenAIRE

    Al-Naima, Fawzi M.; Hamd, Hassan A.

    2012-01-01

    Due to the proliferation in the number of vehicles on the road, traffic problems are bound to exist. Therefore, the use of Intelligent Transportation Systems (ITS) has become mandatory for obtaining traffic information from roads. Radio Frequency Identification (RFID) technology has been used to obtain vehicles’ IDs (tag ID) from RFID readers and to collect traffic information in real‐time. This paper proposes a simulation system for the Vehicle Traffic Congestion Estimation (VTCE) based on R...

  17. Rethinking Traffic Management: Design of Optimizable Networks

    Science.gov (United States)

    2008-06-01

    traffic traverses each path in a network. On the Internet today, end hosts run congestion control to adapt sending rates, routers route traffic on...congestion control to adapt sending rates, routers route traffic on shortest paths based on link weights, and operators tune link weights to direct traffic...Chaintreau and Christophe Diot for giving me the opportunity to intern at Thomson Research Labs in Paris. Thanks to all the interns there for v making it

  18. Highway traffic model-based density estimation

    OpenAIRE

    Morarescu, Irinel - Constantin; CANUDAS DE WIT, Carlos

    2011-01-01

    International audience; The travel time spent in traffic networks is one of the main concerns of the societies in developed countries. A major requirement for providing traffic control and services is the continuous prediction, for several minutes into the future. This paper focuses on an important ingredient necessary for the traffic forecasting which is the real-time traffic state estimation using only a limited amount of data. Simulation results illustrate the performances of the proposed ...

  19. AN EFFECTIVE MODEL TO EVALUATE BLOCKING PROBABILITY OF TIME-SLOTTED OPTICAL BURST SWITCHED NETWORKS

    Institute of Scientific and Technical Information of China (English)

    Yang Zongkai; Ou Liang; Tan Xiansi

    2006-01-01

    Time-slotted optical burst switched network is a potential technique to support IP over Wavelength Division Multiplexing (WDM) by introduce Time Division Multiplexing (TDM) channel to Optical Burst Switching (OBS) technology. This paper presents a framework to evaluate blocking performance of time-slotted OBS networks with multi-fiber wavelength channels. The proposed model is efficient for not only single class traffic such as individual circuit switch traffics or best-effort traffics but also mixed multi-class traffics.The effectiveness of the proposed model is validated by simulation results. The study shows that blocking performance of multi-fiber TS-OBS network is acceptable for future Internet services.

  20. Probabilistic description of traffic breakdowns.

    Science.gov (United States)

    Kühne, Reinhart; Mahnke, Reinhard; Lubashevsky, Ihor; Kaupuzs, Jevgenijs

    2002-06-01

    We analyze the characteristic features of traffic breakdown. To describe this phenomenon we apply the probabilistic model regarding the jam emergence as the formation of a large car cluster on a highway. In these terms, the breakdown occurs through the formation of a certain critical nucleus in the metastable vehicle flow, which enables us to confine ourselves to one cluster model. We assume that, first, the growth of the car cluster is governed by attachment of cars to the cluster whose rate is mainly determined by the mean headway distance between the car in the vehicle flow and, maybe, also by the headway distance in the cluster. Second, the cluster dissolution is determined by the car escape from the cluster whose rate depends on the cluster size directly. The latter is justified using the available experimental data for the correlation properties of the synchronized mode. We write the appropriate master equation converted then into the Fokker-Planck equation for the cluster distribution function and analyze the formation of the critical car cluster due to the climb over a certain potential barrier. The further cluster growth irreversibly causes jam formation. Numerical estimates of the obtained characteristics and the experimental data of the traffic breakdown are compared. In particular, we draw a conclusion that the characteristic intrinsic time scale of the breakdown phenomenon should be about 1 min and explain the case why the traffic volume interval inside which traffic breakdown is observed is sufficiently wide.

  1. The role of traffic rules.

    NARCIS (Netherlands)

    Noordzij, P.C.

    1988-01-01

    Experienced road users seem to have their own set of traffic rules (including rules about when to violate the official rules). The number of violations is enormous, causing great concern for the authorities. The situation could be improved by separating a set of rules with the aim of deterring road

  2. Traffic Congestion and Congestion Pricing

    OpenAIRE

    Verhoef, E.T.; Lindsey, C.R.

    2000-01-01

    For several decades growth of traffic volumes has outstrippedinvestments inroad infrastructure. The result has been a relentless increase intrafficcongestion. This paper reviews the economic principles behindcongestionpricing in static and dynamic settings, which derive from thebenefits ofcharging travellers for the externalities they create. Specialattention ispaid to various complications that make simple textbook congestionpricingmodels of limited relevance, and dictate that congestion pri...

  3. Learning to Detect Traffic Signs

    DEFF Research Database (Denmark)

    Møgelmose, Andreas; Trivedi, Mohan M.; Moeslund, Thomas B.

    2012-01-01

    This study compares the performance of sign detection based on synthetic training data to the performance of detection based on real-world training images. Viola-Jones detectors are created for 4 different traffic signs with both synthetic and real data, and varying numbers of training samples. T...

  4. Computational intelligence for traffic and mobility

    CERN Document Server

    Wang, Wuhong

    2012-01-01

    This book presents the new development of computation intelligence for traffic, transportation and mobility, the main contents include traffic safety, mobility analysis, intelligent transportation system, smart vehicle, transportation behavior, driver modeling and assistance, transportation risk analysis and reliability system analysis, vehicle operation and active safety, urban traffic management and planning.

  5. Prediction of Railway Passenger Traffic Volume

    Institute of Scientific and Technical Information of China (English)

    2001-01-01

    The current situation of the railway passenger traffic (RPT) andthe traffic marketing is analyzed. The grey model theory is adopted to establish a prediction model for the railway passenger traffic volume (RPTV). The RPTV from 2001 to 2005 is predicted with the proposed model, and a few suggestions are put forward.

  6. Capturing Internet Traffic Dynamics through Graph Distances

    NARCIS (Netherlands)

    Uhlig, S.; Fu, B.; Jamakovic, A.

    2009-01-01

    Studies of the Internet have typically focused either on the routing system, i.e. the paths chosen to reach a given destination, or on the evolution of traffic on a physical link. In this paper, we combine routing and traffic, and study for the first time the evolution of the traffic on the Internet

  7. Light signals for road traffic control.

    NARCIS (Netherlands)

    Schreuder, D.A.

    1981-01-01

    Signals for road traffic control are a major constituent of the modern traffic scene, particularly in built-up areas. A vast amount of research has been executed in the last two decennia, resulting in a fairly generally accepted view on what the requirements for effective traffic lights are. For the

  8. Disseminating Traffic Information in Vehicular Networks

    Directory of Open Access Journals (Sweden)

    Muhammad Alyas Shahid

    2015-11-01

    Full Text Available In this paper Wifi based system is described for disseminating traffic information in vehicular networks. Basic aim of this approach is to distribute information regarding the traffic conditions such as traffic congestions or safety warnings to vehicles in an effective way.

  9. 15 CFR 265.22 - Bicycle traffic.

    Science.gov (United States)

    2010-01-01

    ... 15 Commerce and Foreign Trade 1 2010-01-01 2010-01-01 false Bicycle traffic. 265.22 Section 265.22... Bicycle traffic. No person shall ride a bicycle other than in a manner exercising due caution for pedestrian and other traffic. No person shall ride a bicycle on sidewalks or inside any building, nor...

  10. 30 CFR 56.9100 - Traffic control.

    Science.gov (United States)

    2010-07-01

    ... 30 Mineral Resources 1 2010-07-01 2010-07-01 false Traffic control. 56.9100 Section 56.9100 Mineral Resources MINE SAFETY AND HEALTH ADMINISTRATION, DEPARTMENT OF LABOR METAL AND NONMETAL MINE... Dumping Traffic Safety § 56.9100 Traffic control. To provide for the safe movement of...

  11. Some random models in traffic science

    Energy Technology Data Exchange (ETDEWEB)

    Hjorth, U.

    1996-06-01

    We give an overview of stochastic models for the following traffic phenomena. Models for traffic flow including gaps and capacities for lanes, crossings and roundabouts. Models for wanted and achieved speed distributions. Mode selection models including dispersed equilibrium models and traffic accident models. Also some statistical questions are discussed. 60 refs, 1 tab

  12. Empirical analysis of heterogeneous traffic flow

    NARCIS (Netherlands)

    Ambarwati, L.; Pel, A.J.; Verhaeghe, R.J.; Van Arem, B.

    2013-01-01

    Traffic flow in many developing countries is strongly mixed comprising vehicle types, such as motorcycles, cars, (mini) buses, and trucks; furthermore, traffic flow typically exhibits free inter-lane exchanges. This phenomenon causes a complex vehicle interaction, rendering most existing traffic flo

  13. Models for Predictive Railway Traffic Management

    NARCIS (Netherlands)

    Kecman, P.

    2014-01-01

    The potential growth in transport demand in the next decade and beyond requires a change from reactive to proactive traffic control to maintain and improve the reliability of railway traffic. In order to enable an anticipative approach to traffic management, it is necessary to develop the tools for

  14. Phase diagram distortion from traffic parameter averaging.

    NARCIS (Netherlands)

    Stipdonk, H. Toorenburg, J. van & Postema, M.

    2010-01-01

    Motorway traffic congestion is a major bottleneck for economic growth. Therefore, research of traffic behaviour is carried out in many countries. Although well describing the undersaturated free flow phase as an almost straight line in a (k,q)-phase diagram, congested traffic observations and theori

  15. NEW POSSIBILITIES OF RAILWAY TRAFFIC CONTROL SYSTEMS

    Directory of Open Access Journals (Sweden)

    Lionginas LIUDVINAVIČIUS

    2016-06-01

    Full Text Available This article analyses the train traffic control systems in 1435 mm and 1520 mm gauge railways. The article analyses the aspects of train traffic control and locomotive energy saving by using the coordinates of track profile change that have been received from GPS. In the article, achievements of Lithuanian railways (LG in the area of train traffic control optimisation are presented.

  16. Traffic Measurement for Traffic Engineering in IP/MPLS Based Telecom Core Networks

    Institute of Scientific and Technical Information of China (English)

    2002-01-01

    Telecommunication networks will become IP-based networks with all voice, data and multimedia traffic carried as packets with appropriate quality and class of service for each traffic type. It is very important for service provider to apply Traffic Engineering (TE) with the dramatic growth of networks traffic. In this paper, we discuss traffic engineering based on IP/MPLS and emphasize traffic measurement in telecom core networks. We specify the technology and scientific principles of the measurement, modeling, characterization, and the traffic flow matrix. The paper concludes with a preliminary analysis of TE measurement and a discussion about the development of TE measurement.

  17. A traffic model of optical networks based on time-space complexity and traffic grooming

    Institute of Scientific and Technical Information of China (English)

    Zhao Yongli; Zhang Jie; Han Dahai; Wang Lei; Chen Xiuzhong; Gu Wanyi

    2009-01-01

    This paper researched the traffic of optical networks in time-space complexity, proposed a novel traffic model for complex optical networks based on traffic grooming, designed a traffic generator GTS (generator based on time and space) with "centralized + distributed" idea, and then made a simulation in C language. Experiments results show that GTS can produce the virtual network topology which can change dynamically with the characteristic of scaling-free network. GTS can also groom the different traffic and trigger them under real-time or scheduling mechanisms, generating different optical connections. This traffic model is convenient for the simulation of optical networks considering the traffic complexity.

  18. Traffic fatalities and economic growth.

    Science.gov (United States)

    Kopits, Elizabeth; Cropper, Maureen

    2005-01-01

    This paper examines the relationship between traffic fatality risk and per capita income and uses it to forecast traffic fatalities by geographic region. Equations for the road death rate (fatalities/population) and its components--the rate of motorization (vehicles/population) and fatalities per vehicle (F/V)--are estimated using panel data from 1963 to 1999 for 88 countries. The natural logarithm of F/P, V/P, and F/V are expressed as spline (piecewise linear) functions of the logarithm of real per capita GDP (measured in 1985 international prices). Region-specific time trends during the period 1963-1999 are modeled in linear and log-linear form. These models are used to project traffic fatalities and the stock of motor vehicles to 2020. The per capita income at which traffic fatality risk (fatalities/population) begins to decline is 8600 US dollars (1985 international dollars) when separate time trends are used for each geographic region. This turning point is driven by the rate of decline in fatalities/vehicles as income rises since vehicles/population, while increasing with income at a decreasing rate, never declines with economic growth. Projections of future traffic fatalities suggest that the global road death toll will grow by approximately 66% over the next twenty years. This number, however, reflects divergent rates of change in different parts of the world: a decline in fatalities in high-income countries of approximately 28% versus an increase in fatalities of almost 92% in China and 147% in India. The road death rate is projected to rise to approximately 2 per 10,000 persons in developing countries by 2020, while it will fall to less than 1 per 10,000 in high-income countries.

  19. Blocking-resistant communication through domain fronting

    Directory of Open Access Journals (Sweden)

    Fifield David

    2015-06-01

    Full Text Available We describe “domain fronting,” a versatile censorship circumvention technique that hides the remote endpoint of a communication. Domain fronting works at the application layer, using HTTPS, to communicate with a forbidden host while appearing to communicate with some other host, permitted by the censor. The key idea is the use of different domain names at different layers of communication. One domain appears on the “outside” of an HTTPS request—in the DNS request and TLS Server Name Indication—while another domain appears on the “inside”—in the HTTP Host header, invisible to the censor under HTTPS encryption. A censor, unable to distinguish fronted and nonfronted traffic to a domain, must choose between allowing circumvention traffic and blocking the domain entirely, which results in expensive collateral damage. Domain fronting is easy to deploy and use and does not require special cooperation by network intermediaries. We identify a number of hard-to-block web services, such as content delivery networks, that support domain-fronted connections and are useful for censorship circumvention. Domain fronting, in various forms, is now a circumvention workhorse. We describe several months of deployment experience in the Tor, Lantern, and Psiphon circumvention systems, whose domain-fronting transports now connect thousands of users daily and transfer many terabytes per month.

  20. BLOCK H-MATRICES AND SPECTRUM OF BLOCK MATRICES

    Institute of Scientific and Technical Information of China (English)

    黄廷祝; 黎稳

    2002-01-01

    The block H-matrices are studied by the concept of G-functions, several concepts of block matrices are introduced. Equivalent characters of block H-matrices are obtained. Spectrum localizations claracterized by Gfunctions for block matrices are got.

  1. Block TERM factorization of block matrices

    Institute of Scientific and Technical Information of China (English)

    SHE Yiyuan; HAO Pengwei

    2004-01-01

    Reversible integer mapping (or integer transform) is a useful way to realize Iossless coding, and this technique has been used for multi-component image compression in the new international image compression standard JPEG 2000. For any nonsingular linear transform of finite dimension, its integer transform can be implemented by factorizing the transform matrix into 3 triangular elementary reversible matrices (TERMs) or a series of single-row elementary reversible matrices (SERMs). To speed up and parallelize integer transforms, we study block TERM and SERM factorizations in this paper. First, to guarantee flexible scaling manners, the classical determinant (det) is generalized to a matrix function, DET, which is shown to have many important properties analogous to those of det. Then based on DET, a generic block TERM factorization,BLUS, is presented for any nonsingular block matrix. Our conclusions can cover the early optimal point factorizations and provide an efficient way to implement integer transforms for large matrices.

  2. Traffic Modeling in WCDMA System Level Simulations

    Institute of Scientific and Technical Information of China (English)

    2002-01-01

    Traffic modeling is a crucial element in WCDMA system level simulations. A clear understanding of the nature of traffic in the WCDMA system and subsequent selection of an appropriate random traffic model are critical to the success of the modeling enterprise. The resultant performances will evidently be of a function that our design has been well adapted to the traffic, channel and user mobility models, and these models are also accurate. In this article, our attention will be focused on modeling voice and WWW data traffic with the SBBP model and Victor model respectively.

  3. Characterization and Modeling of Network Traffic

    DEFF Research Database (Denmark)

    Shawky, Ahmed; Bergheim, Hans; Ragnarsson, Olafur

    2011-01-01

    This paper attempts to characterize and model backbone network traffic, using a small number of statistics. In order to reduce cost and processing power associated with traffic analysis. The parameters affecting the behaviour of network traffic are investigated and the choice is that inter......-arrival time, IP addresses, port numbers and transport protocol are the only necessary parameters to model network traffic behaviour. In order to recreate this behaviour, a complex model is needed which is able to recreate traffic behaviour based on a set of statistics calculated from the parameters values...

  4. Lesson Thirteen Trifascicular Block

    Institute of Scientific and Technical Information of China (English)

    鲁端; 王劲

    2005-01-01

    @@ A complete trifascicular block would result in complete AV block. The idio ventricular rhythm has a slower rate and a wide QRS complex because the pacemaker is located at the peripheral part of the conduction system distal to the sites of the block1. Such a rhythm may be difficult to differentiate from bifascicular or bundle branch block combined with complete block at a higher level such as the AV node or His bundle2. Besides a slower ventricular rate, a change in the morphology of the QRS complex from a previous known bifascicular pattern would be strongly suggestive of a trifascicular origin of the complete AV block3. A His bundle recording is required for a definitive diagnosis, however.

  5. Bandwidth constrained QoS routing scheme for MPLS traffic engineering

    Institute of Scientific and Technical Information of China (English)

    李震宇; 张中兆

    2004-01-01

    Traffic engineering has been essential to optimize the utilization of network resource and to provide for QoS,QoS routing is a basic component of traffic engineering in MPLS networks. Aiming at some shortages of certain existing routing algorithms, we propose an efficient bandwidth constrained QoS routing scheme with additional traffic engineering objectives. The objectives of traffic engineering considered in our algorithm are to reduce the blocking probability of requests and to evenly distribute the traffic load. Accordingly, the algorithm avoids using critical links as far as possible by assigning large weights to them as a function of their criticality. The critical links can be specified according to max-flow reduction weight, a new link weight introduced by the algorithm. Then, a new defined additive metric, path saturation,is used to select optimal path. Extensive simulations are carried out to evaluate the performance of the proposed algorithm in terms of call blocking probability and link resource utilization. Compared to other several routing algorithms, the simulation results show the new algorithm performs well.

  6. Automated selection of regions of interest for intensity-based FRET analysis of transferrin endocytic trafficking in normal vs. cancer cells.

    Science.gov (United States)

    Talati, Ronak; Vanderpoel, Andrew; Eladdadi, Amina; Anderson, Kate; Abe, Ken; Barroso, Margarida

    2014-03-15

    The overexpression of certain membrane-bound receptors is a hallmark of cancer progression and it has been suggested to affect the organization, activation, recycling and down-regulation of receptor-ligand complexes in human cancer cells. Thus, comparing receptor trafficking pathways in normal vs. cancer cells requires the ability to image cells expressing dramatically different receptor expression levels. Here, we have presented a significant technical advance to the analysis and processing of images collected using intensity based Förster resonance energy transfer (FRET) confocal microscopy. An automated Image J macro was developed to select region of interests (ROI) based on intensity and statistical-based thresholds within cellular images with reduced FRET signal. Furthermore, SSMD (strictly standardized mean differences), a statistical signal-to-noise ratio (SNR) evaluation parameter, was used to validate the quality of FRET analysis, in particular of ROI database selection. The Image J ROI selection macro together with SSMD as an evaluation parameter of SNR levels, were used to investigate the endocytic recycling of Tfn-TFR complexes at nanometer range resolution in human normal vs. breast cancer cells expressing significantly different levels of endogenous TFR. Here, the FRET-based assay demonstrates that Tfn-TFR complexes in normal epithelial vs. breast cancer cells show a significantly different E% behavior during their endocytic recycling pathway. Since E% is a relative measure of distance, we propose that these changes in E% levels represent conformational changes in Tfn-TFR complexes during endocytic pathway. Thus, our results indicate that Tfn-TFR complexes undergo different conformational changes in normal vs. cancer cells, indicating that the organization of Tfn-TFR complexes at the nanometer range is significantly altered during the endocytic recycling pathway in cancer cells. In summary, improvements in the automated selection of FRET ROI datasets

  7. Urban traffic congestion propagation and bottleneck identification

    Institute of Scientific and Technical Information of China (English)

    LONG JianCheng; GAO ZiYou; REN HuaLing; LIAN AiPing

    2008-01-01

    Bottlenecks in urban traffic network are sticking points in restricting network col-lectivity traffic efficiency.To identify network bottlenecks effectively is a founda-tional work for improving network traffic condition and preventing traffic conges-tion.In this paper,a congestion propagation model of urban network traffic is proposed based on the cell transmission model (CTM).The proposed model in-cludes a link model,which describes flow propagation on links,and a node model,which represents link-to-link flow propagation.A new method of estimating average journey velocity (AJV) of both link and network is developed to identify network congestion bottlenecks.A numerical example is studied in Sioux Falls urban traffic network.The proposed model is employed in simulating network traffic propaga-tion and congestion bottleneck identification under different traffic demands.The simulation results show that continual increase of traffic demand is an immediate factor in network congestion bottleneck emergence and increase as well as re-ducing network collectivity capability.Whether a particular link will become a bot-tleneck is mainly determined by its position in network,its traffic flow (attributed to different OD pairs) component,and network traffic demand.

  8. The car following model considering traffic jerk

    Science.gov (United States)

    Ge, Hong-Xia; Zheng, Peng-jun; Wang, Wei; Cheng, Rong-Jun

    2015-09-01

    Based on optimal velocity car following model, a new model considering traffic jerk is proposed to describe the jamming transition in traffic flow on a highway. Traffic jerk means the sudden braking and acceleration of vehicles, which has a significant impact on traffic movement. The nature of the model is researched by using linear and nonlinear analysis method. A thermodynamic theory is formulated to describe the phase transition and critical phenomenon in traffic flow. The time-dependent Ginzburg-Landau (TDGL) equation and the modified Korteweg-de Vries (mKdV) equation are derived to describe the traffic flow near the critical point and the traffic jam. In addition, the connection between the TDGL and the mKdV equations are also given.

  9. Endocytic Recycling Proteins EHD1 and EHD2 Interact with Fer-1-like-5 (Fer1L5) and Mediate Myoblast Fusion*

    Science.gov (United States)

    Posey, Avery D.; Pytel, Peter; Gardikiotes, Konstantina; Demonbreun, Alexis R.; Rainey, Mark; George, Manju; Band, Hamid; McNally, Elizabeth M.

    2011-01-01

    The mammalian ferlins are calcium-sensing, C2 domain-containing proteins involved in vesicle trafficking. Myoferlin and dysferlin regulate myoblast fusion and muscle membrane resealing, respectively. Correspondingly, myoferlin is most highly expressed in singly nucleated myoblasts, whereas dysferlin expression is increased in mature, multinucleated myotubes. Myoferlin also mediates endocytic recycling and participates in trafficking the insulin-like growth factor receptor. We have now characterized a novel member of the ferlin family, Fer1L5, because of its high homology to dysferlin and myoferlin. We found that Fer1L5 protein is expressed in small myotubes that contain only two to four nuclei. We also found that Fer1L5 protein binds directly to the endocytic recycling proteins EHD1 and EHD2 and that the second C2 domain in Fer1L5 mediates this interaction. Reduction of EHD1 and/or EHD2 inhibits myoblast fusion, and EHD2 is required for normal translocation of Fer1L5 to the plasma membrane. The characterization of Fer1L5 and its interaction with EHD1 and EHD2 underscores the complex requirement of ferlin proteins and mediators of endocytic recycling for membrane trafficking events during myotube formation. PMID:21177873

  10. Endocytic recycling proteins EHD1 and EHD2 interact with fer-1-like-5 (Fer1L5) and mediate myoblast fusion.

    Science.gov (United States)

    Posey, Avery D; Pytel, Peter; Gardikiotes, Konstantina; Demonbreun, Alexis R; Rainey, Mark; George, Manju; Band, Hamid; McNally, Elizabeth M

    2011-03-01

    The mammalian ferlins are calcium-sensing, C2 domain-containing proteins involved in vesicle trafficking. Myoferlin and dysferlin regulate myoblast fusion and muscle membrane resealing, respectively. Correspondingly, myoferlin is most highly expressed in singly nucleated myoblasts, whereas dysferlin expression is increased in mature, multinucleated myotubes. Myoferlin also mediates endocytic recycling and participates in trafficking the insulin-like growth factor receptor. We have now characterized a novel member of the ferlin family, Fer1L5, because of its high homology to dysferlin and myoferlin. We found that Fer1L5 protein is expressed in small myotubes that contain only two to four nuclei. We also found that Fer1L5 protein binds directly to the endocytic recycling proteins EHD1 and EHD2 and that the second C2 domain in Fer1L5 mediates this interaction. Reduction of EHD1 and/or EHD2 inhibits myoblast fusion, and EHD2 is required for normal translocation of Fer1L5 to the plasma membrane. The characterization of Fer1L5 and its interaction with EHD1 and EHD2 underscores the complex requirement of ferlin proteins and mediators of endocytic recycling for membrane trafficking events during myotube formation.

  11. A membrane microdomain-associated protein, Arabidopsis Flot1, is involved in a clathrin-independent endocytic pathway and is required for seedling development.

    Science.gov (United States)

    Li, Ruili; Liu, Peng; Wan, Yinglang; Chen, Tong; Wang, Qinli; Mettbach, Ursula; Baluska, Frantisek; Samaj, Jozef; Fang, Xiaohong; Lucas, William J; Lin, Jinxing

    2012-05-01

    Endocytosis is essential for the maintenance of protein and lipid compositions in the plasma membrane and for the acquisition of materials from the extracellular space. Clathrin-dependent and -independent endocytic processes are well established in yeast and animals; however, endocytic pathways involved in cargo internalization and intracellular trafficking remain to be fully elucidated for plants. Here, we used transgenic green fluorescent protein-flotillin1 (GFP-Flot1) Arabidopsis thaliana plants in combination with confocal microscopy analysis and transmission electron microscopy immunogold labeling to study the spatial and dynamic aspects of GFP-Flot1-positive vesicle formation. Vesicle size, as outlined by the gold particles, was ∼100 nm, which is larger than the 30-nm size of clathrin-coated vesicles. GFP-Flot1 also did not colocalize with clathrin light chain-mOrange. Variable-angle total internal reflection fluorescence microscopy also revealed that the dynamic behavior of GFP-Flot1-positive puncta was different from that of clathrin light chain-mOrange puncta. Furthermore, disruption of membrane microdomains caused a significant alteration in the dynamics of Flot1-positive puncta. Analysis of artificial microRNA Flot1 transgenic Arabidopsis lines established that a reduction in Flot1 transcript levels gave rise to a reduction in shoot and root meristem size plus retardation in seedling growth. Taken together, these findings support the hypothesis that, in plant cells, Flot1 is involved in a clathrin-independent endocytic pathway and functions in seedling development.

  12. An auxiliary graph based dynamic traffic grooming algorithm in spatial division multiplexing enabled elastic optical networks with multi-core fibers

    Science.gov (United States)

    Zhao, Yongli; Tian, Rui; Yu, Xiaosong; Zhang, Jiawei; Zhang, Jie

    2017-03-01

    A proper traffic grooming strategy in dynamic optical networks can improve the utilization of bandwidth resources. An auxiliary graph (AG) is designed to solve the traffic grooming problem under a dynamic traffic scenario in spatial division multiplexing enabled elastic optical networks (SDM-EON) with multi-core fibers. Five traffic grooming policies achieved by adjusting the edge weights of an AG are proposed and evaluated through simulation: maximal electrical grooming (MEG), maximal optical grooming (MOG), maximal SDM grooming (MSG), minimize virtual hops (MVH), and minimize physical hops (MPH). Numeric results show that each traffic grooming policy has its own features. Among different traffic grooming policies, an MPH policy can achieve the lowest bandwidth blocking ratio, MEG can save the most transponders, and MSG can obtain the fewest cores for each request.

  13. Car Delay Model near Bus Stops with Mixed Traffic Flow

    Directory of Open Access Journals (Sweden)

    Yang Xiaobao

    2013-01-01

    Full Text Available This paper proposes a model for estimating car delays at bus stops under mixed traffic using probability theory and queuing theory. The roadway is divided to serve motorized and nonmotorized traffic streams. Bus stops are located on the nonmotorized lanes. When buses dwell at the stop, they block the bicycles. Thus, two conflict points between car stream and other traffic stream are identified. The first conflict point occurs as bicycles merge to the motorized lane to avoid waiting behind the stopping buses. The second occurs as buses merge back to the motorized lane. The average car delay is estimated as the sum of the average delay at these two conflict points and the delay resulting from following the slower bicycles that merged into the motorized lane. Data are collected to calibrate and validate the developed model from one site in Beijing. The sensitivity of car delay to various operation conditions is examined. The results show that both bus stream and bicycle stream have significant effects on car delay. At bus volumes above 200 vehicles per hour, the curbside stop design is not appropriate because of the long car delays. It can be replaced by the bus bay design.

  14. Traffic engineering for Internet applications

    Science.gov (United States)

    Jena, Ajit K.; Popescu, Adrian

    2001-07-01

    The focus of the paper is on resource engineering for supporting Service Level Agreements (SLAs) in IP networks. SLA at both link level and application level are considered. Using an object-oriented simulation model a case study is presented for client-server interactions generated by mixed traffic conditions in a Frame Relay (FR) WAN. Performance issues of Short Range Dependence and Long Range Dependence traffic under different resource control regimes are compared. The results show that major portion of the end-to- end delay comes from the queueing delay at the WAN ingress points, which is due to the significant bandwidth differences that may exist between LAN and WAN link layers. The results also highlight the role TCP window size and FR PVC control mechanisms play in the provision of delay performance for Internet services.

  15. Agent Based Individual Traffic guidance

    DEFF Research Database (Denmark)

    Wanscher, Jørgen Bundgaard

    2004-01-01

    When working with traffic planning or guidance it is common practice to view the vehicles as a combined mass. >From this models are employed to specify the vehicle supply and demand for each region. As the models are complex and the calculations are equally demanding the regions and the detail...... can be obtained through cellular phone tracking or GPS systems. This information can then be used to provide individual traffic guidance as opposed to the mass information systems of today -- dynamic roadsigns and trafficradio. The goal is to achieve better usage of road and time. The main topic...... of the paper is the possibilities of using ABIT when disruptions occur (accidents, congestion, and roadwork). The discussion will be based on realistic case studies....

  16. Crowding effects in vehicular traffic.

    Science.gov (United States)

    Combinido, Jay Samuel L; Lim, May T

    2012-01-01

    While the impact of crowding on the diffusive transport of molecules within a cell is widely studied in biology, it has thus far been neglected in traffic systems where bulk behavior is the main concern. Here, we study the effects of crowding due to car density and driving fluctuations on the transport of vehicles. Using a microscopic model for traffic, we found that crowding can push car movement from a superballistic down to a subdiffusive state. The transition is also associated with a change in the shape of the probability distribution of positions from a negatively-skewed normal to an exponential distribution. Moreover, crowding broadens the distribution of cars' trap times and cluster sizes. At steady state, the subdiffusive state persists only when there is a large variability in car speeds. We further relate our work to prior findings from random walk models of transport in cellular systems.

  17. Crowding effects in vehicular traffic

    CERN Document Server

    Combinido, Jay Samuel L

    2012-01-01

    While the impact of crowding on the diffusive transport of molecules within a cell is widely studied in biology, it has thus far been neglected in traffic systems where bulk behavior is the main concern. Here, we study the effects of crowding due to car density and driving fluctuations on the transport of vehicles. Using a microscopic model for traffic, we found that crowding can push car movement from a superballistic down to a subdiffusive state. The transition is also associated with a change in the shape of the probability distribution of positions from negatively-skewed normal to an exponential distribution. Moreover, crowding broadens the distribution of cars' trap times and cluster sizes. At steady state, the subdiffusive state persists only when there is a large variability in car speeds. We further relate our work to prior findings from random walk models of transport in cellular systems.

  18. Container Traffic In European Ports

    Directory of Open Access Journals (Sweden)

    Elen Twrdy

    2004-03-01

    Full Text Available Over the last fifteen years the European transport markethas witnessed a growth of container traffic which today reachesapproximately 50 million TEU per year. From 1997 to 2002,container traffic in the northern European ports increased from14 to 20.6 million TEU per year, in the ports of the westernMedite"anean from 6 to 10 million TEU per year, and in thenorthern Adriatic ports from 0. 69 to 0. 74 million TEU per year.The ports of the northern Adriatic are located in three states(Slovenia, Croatia and Italy with different statuses in relationto the common European market. In addition, different developmentlevels of these states are reflected in different levels ofinternational commercial exchange, the development of the existinginfrastructure and plans for the construction of new infrastructures.However, all three countries share a common goaltoincrease their competitiveness in comparison with the westemEuropean ports.

  19. Real-time studies of the interactions between epidermal growth factor and its receptor during endocytic trafficking.

    Science.gov (United States)

    Martin-Fernandez, M L; Clarke, D T; Tobin, M J; Jones, G R

    2000-09-01

    The interactions of growth factors with cell surface receptors regulate fundamental cell processes, such as growth, differentiation and transformation. Understanding the nature of these interactions at the molecular level is of fundamental importance in cell biology. This is not only from the point of view of basic science, but also because of the repercussions such knowledge might have in understanding the mode of action of drugs in cells. Receptor mediated endocytosis has been implicated in the downregulation of the mitogenic signal. However, no data are thus far available on how growth factor/receptor interactions might control endocytic trafficking. Here we show that information on modes of binding and receptor conformational changes can be obtained using time-resolved fluorescence methods. We have found that fluorescent probes bound to epidermal growth factor (EGF) show dynamic fluorescence quenching when EGF is bound to internalising EGF receptors (EGFR). We propose that this dynamic quenching takes place because EGF-bound probes interact with tryptophan residues in the extracellular domain of the EGF-EGFR complex. Real-time accumulation of fluorescent decays has also allowed us to follow the time course of a conformational change in EGFR occurring during endocytosis, and correlate this information with endosomal trafficking and EGFR recycling.

  20. Oncogenic activation of the Met receptor tyrosine kinase fusion protein, Tpr-Met, involves exclusion from the endocytic degradative pathway.

    Science.gov (United States)

    Mak, H H L; Peschard, P; Lin, T; Naujokas, M A; Zuo, D; Park, M

    2007-11-01

    Multiple mechanisms of dysregulation of receptor tyrosine kinases (RTKs) are observed in human cancers. In addition to gain-of-function, loss of negative regulation also contributes to oncogenic activation of RTKs. Negative regulation of many RTKs involves their internalization and degradation in the lysosome, a process regulated through ubiquitination. RTK oncoproteins activated following chromosomal translocation, are no longer transmembrane proteins, and are predicted to escape lysosomal degradation. To test this, we used the Tpr-Met oncogene, generated following chromosomal translocation of the hepatocyte growth factor receptor (Met). Unlike Met, Tpr-Met is localized in the cytoplasm and also lacks the binding site for Cbl ubiquitin ligases. We determined whether subcellular localization of Tpr-Met, and/or loss of its Cbl-binding site, is important for oncogenic activity. Presence of a Cbl-binding site and ubiquitination of cytosolic Tpr-Met oncoproteins does not alter their transforming activity. In contrast, plasma membrane targeting allows Tpr-Met to enter the endocytic pathway, and Tpr-Met transforming activity as well as protein stability are decreased in a Cbl-dependent manner. We show that transformation by Tpr-Met is in part dependent on its ability to escape normal downregulatory mechanisms. This provides a paradigm for many RTK oncoproteins activated following chromosomal translocation.

  1. Traffic control: nursing practice calendar.

    Science.gov (United States)

    Rus, Linda; Cheesebro, Kathy; Nagra, Erica; Neff, Alaina

    2013-01-01

    Educating nurses on the multitude of new and updated best practices, changes in regulatory standards, new equipment, and enhanced technology creates an "information traffic jam." Multiple practice changes occurring simultaneously pose challenges for nurses to retain information to practice safely and effectively. An absence of coordination between various nursing and allied health teaching initiatives compounds this problem. A nursing practice calendar was developed to facilitate the prioritization, communication, and education of hospital-wide initiatives affecting nursing practice.

  2. Personal Property Traffic Management Regulation

    Science.gov (United States)

    1991-10-01

    transportation in domestic, domestic ( offshore ), and international traffic and determine the DOD interest and action required within the scope of the DOD...S. 0MRS 4D. LMLISERMU3S TEMPORARY j~i~ o~ ~l h PAYING P WO/F AONAWV AND MAROC O CRPS 13 . em e L a.C*KT Cn1%f ~m STAYSOF L*OAL RIQENCS 7. EmnTIEMENTS

  3. Development of Deflection Prediction Model for Concrete Block Pavement Considering the Block Shapes and Construction Patterns

    Directory of Open Access Journals (Sweden)

    Wuguang Lin

    2016-01-01

    Full Text Available Concrete block pavement (CBP is distinct from typical concrete or asphalt pavements. It is built by using individual blocks with unique construction patterns forming a discrete surface layer to bear traffic loadings. The surface structure of CBP varies depending on the block shapes and construction patterns, so it is hard to apply a general equivalent elastic modulus estimation method to define the surface structural strength. In this study, FEM analysis and dynamic loading test were carried out to develop a deflection prediction model for CBP considering the block shapes and construction patterns. Based on the analysis results, it was found that block shapes did not have much effect on load distribution, whereas construction patterns did. By applying the deflection prediction model to the rutting model for CBP proposed by Sun, the herringbone bond pattern showed the best performance comparing with stretcher bond or basket weave bond pattern. As the load repetition increased to 1.2 million, the rutting depth of CBP constructed by herringbone bond pattern was 2 mm smaller than those constructed by the other two patterns.

  4. Transition characteristic analysis of traffic evolution process for urban traffic network.

    Science.gov (United States)

    Wang, Longfei; Chen, Hong; Li, Yang

    2014-01-01

    The characterization of the dynamics of traffic states remains fundamental to seeking for the solutions of diverse traffic problems. To gain more insights into traffic dynamics in the temporal domain, this paper explored temporal characteristics and distinct regularity in the traffic evolution process of urban traffic network. We defined traffic state pattern through clustering multidimensional traffic time series using self-organizing maps and construct a pattern transition network model that is appropriate for representing and analyzing the evolution progress. The methodology is illustrated by an application to data flow rate of multiple road sections from Network of Shenzhen's Nanshan District, China. Analysis and numerical results demonstrated that the methodology permits extracting many useful traffic transition characteristics including stability, preference, activity, and attractiveness. In addition, more information about the relationships between these characteristics was extracted, which should be helpful in understanding the complex behavior of the temporal evolution features of traffic patterns.

  5. Outer Space Traffic Safety Standards

    Science.gov (United States)

    Larsen, Paul B.

    2013-09-01

    Management of traffic in outer space is a major safety problem. Traffic is increasing. Most satellites are navigable but they have to co-exist with space debris which is not navigable. We need minimum safety rules for outer space traffic. We have the possible beginnings of international safety standards in the form of national space object tracking; Global Navigation Satellite Systems (GNSS) standardization through ICAO and the International Committee on GNSS (ICG); the IADC space debris guidelines; and the proposed Code of Conduct. However, safety could be improved by standards for such activities as licensing launches of satellites into outer space; standards for accident investigation and search and rescue: operational safety zones around space objects such as the International Space Station. This paper describes legal authority for minimum safety standards. It considers safety standards established by private agreements among commercial operators. Finally it examines a number of options for an international forum to establish safety standards, including self-regulation, COPUOS, ICAO, ITU, a space code of conduct, and a new space organization.

  6. Blocked Urethral Valves

    Science.gov (United States)

    ... Blocked Urethral Valves Health Issues Listen Español Text Size Email Print Share Blocked Urethral Valves Page Content Article Body Urine leaves the bladder through a tube called the urethra, which in boys passes through the penis. Rarely, small membranes form across the urethra in ...

  7. Types of Heart Block

    Science.gov (United States)

    ... P wave as it normally would. If an electrical signal is blocked before it reaches the ventricles, they won't contract and pump blood to the lungs and the rest of the body. Second-degree heart block is divided into two ...

  8. Related Drupal Nodes Block

    NARCIS (Netherlands)

    Van der Vegt, Wim

    2010-01-01

    Related Drupal Nodes Block This module exposes a block that uses Latent Semantic Analysis (Lsa) internally to suggest three nodes that are relevant to the node a user is viewing. This module performs three tasks. 1) It periodically indexes a Drupal site and generates a Lsa Term Document Matrix. Inde

  9. The Block Neighborhood

    CERN Document Server

    Arrighi, Pablo

    2010-01-01

    We define the block neighborhood of a reversible CA, which is related both to its decomposition into a product of block permutations and to quantum computing. We give a purely combinatorial characterization of the block neighborhood, which helps in two ways. First, it makes the computation of the block neighborhood of a given CA relatively easy. Second, it allows us to derive upper bounds on the block neighborhood: for a single CA as function of the classical and inverse neighborhoods, and for the composition of several CAs. One consequence of that is a characterization of a class of "elementary" CAs that cannot be written as the composition of two simpler parts whose neighborhoods and inverse neighborhoods would be reduced by one half.

  10. Traffic Monitoring without single Car Detection from optical airborne Images

    OpenAIRE

    Zeller, Klaus; Hinz, Stefan; Rosenbaum, Dominik; Leitloff, Jens; Reinartz, Peter

    2009-01-01

    This article describes several methods for traffic monitoring from airborne optical remote sensing data. These methods classify the traffic into free flowing traffic, traffic congestion and traffic jam. Furthermore a method is explained, which provides information about the average speed of dense traffic on a defined part of the road. All methods gather the information directly from image features, without the use of single vehicle detection. The classification of the traffic is done by st...

  11. Demand-oriented traffic management for incidents and disasters

    OpenAIRE

    Flötteröd, Yun-Pang; Bieker, Laura

    2012-01-01

    Traffic simulation has been extensively used as a decision support tool for efficient traffic management in daily life. During disasters and incidents, traffic simulation can further help rescue teams to understand the current traffic state and the possible impacts of proposed strategies and then to make proper decisions. At this point, the changes in traffic demand should also be considered in the simulation to reflect the latest traffic state. In this paper, a demand-oriented traffic manage...

  12. Analysis and Modeling of Traffic in Modern Data Communication Networks

    OpenAIRE

    Babic, G.; Vandalore, B.; Jain, R.

    1998-01-01

    In performance analysis and design of communication netword modeling data traffic is important. With introduction of new applications, the characteristics of the data traffic changes. We present a brief review the different models of data traffic and how they have evolved. We present results of data traffic analysis and simulated traffic, which demonstrates that the packet train model fits the traffic at source destination level and long-memory (self-similar) model fits the traffic at the agg...

  13. An antibody toolkit for the study of membrane traffic in Drosophila melanogaster

    Directory of Open Access Journals (Sweden)

    Falko Riedel

    2016-07-01

    Full Text Available The use of Drosophila melanogaster as a model organism has been pivotal to understanding the developmental processes of metazoans. However, the use of flies for studying subcellular organization is hampered by a paucity of reliable reagents to label specific organelles. Here, we describe the generation of mouse monoclonal antibodies against a set of markers of the secretory and endocytic pathways, along with goat polyclonal antibodies against two Golgi proteins. We show that the monoclonal antibodies are highly specific and sufficiently sensitive to detect endogenous proteins in crude extracts by immunoblotting with little background staining. By immunofluorescence the major compartments of the membrane traffic system (including the endoplasmic reticulum, the Golgi, and early and late endosomes are labeled by at least one antibody. Moreover, the antibodies can be used to label organelles in fly tissues including salivary glands and wing imaginal discs. We anticipate that these antibodies will provide a useful tool kit to facilitate the investigation of how the endomembrane system functions and varies in the diverse tissue types of metazoans.

  14. HYBRID INTERNET TRAFFIC CLASSIFICATION TECHNIQUE1

    Institute of Scientific and Technical Information of China (English)

    Li Jun; Zhang Shunyi; Lu Yanqing; Yan Junrong

    2009-01-01

    Accurate and real-time classification of network traffic is significant to network operation and management such as QoS differentiation, traffic shaping and security surveillance. However, with many newly emerged P2P applications using dynamic port numbers, masquerading techniques, and payload encryption to avoid detection, traditional classification approaches turn to be ineffective. In this paper, we present a layered hybrid system to classify current Internet traffic, motivated by variety of network activities and their requirements of traffic classification. The proposed method could achieve fast and accurate traffic classification with low overheads and robustness to accommodate both known and unknown/encrypted applications. Furthermore, it is feasible to be used in the context of real-time traffic classification. Our experimental results show the distinct advantages of the proposed classification system, compared with the one-step Machine Learning (ML) approach.

  15. Traffic Flow Management Wrap-Up

    Science.gov (United States)

    Grabbe, Shon

    2011-01-01

    Traffic Flow Management involves the scheduling and routing of air traffic subject to airport and airspace capacity constraints, and the efficient use of available airspace. Significant challenges in this area include: (1) weather integration and forecasting, (2) accounting for user preferences in the Traffic Flow Management decision making process, and (3) understanding and mitigating the environmental impacts of air traffic on the environment. To address these challenges, researchers in the Traffic Flow Management area are developing modeling, simulation and optimization techniques to route and schedule air traffic flights and flows while accommodating user preferences, accounting for system uncertainties and considering the environmental impacts of aviation. This presentation will highlight some of the major challenges facing researchers in this domain, while also showcasing recent innovations designed to address these challenges.

  16. Suppressing Redundancy in Wireless Sensor Network Traffic

    Science.gov (United States)

    Abe, Rey; Honiden, Shinichi

    Redundancy suppression is a network traffic compression technique that, by caching recurring transmission contents at receiving nodes, avoids repeatedly sending duplicate data. Existing implementations require abundant memory both to analyze recent traffic for redundancy and to maintain the cache. Wireless sensor nodes at the same time cannot provide such resources due to hardware constraints. The diversity of protocols and traffic patterns in sensor networks furthermore makes the frequencies and proportions of redundancy in traffic unpredictable. The common practice of narrowing down search parameters based on characteristics of representative packet traces when dissecting data for redundancy thus becomes inappropriate. Such difficulties made us devise a novel protocol that conducts a probabilistic traffic analysis to identify and cache only the subset of redundant transfers that yields most traffic savings. We verified this approach to perform close enough to a solution built on exhaustive analysis and unconstrained caching to be practicable.

  17. Traffic and Granular Flow ’07

    CERN Document Server

    Chevoir, François; Gondret, Philippe; Lassarre, Sylvain; Lebacque, Jean-Patrick; Schreckenberg, Michael

    2009-01-01

    This book covers several research fields, all of which deal with transport. Three main topics are treated: road traffic, granular matter, and biological transport. Different points of view, i.e. modelling, simulations, experiments, and phenomenological observations, are considered. Sub-topics include: highway or urban vehicular traffic (dynamics of traffic, macro/micro modelling, measurements, data analysis, security issues, psychological issues), pedestrian traffic, animal traffic (e.g. social insects), collective motion in biological systems (molecular motors...), granular flow (dense flows, intermittent flows, solid/liquid transition, jamming, force networks, fluid and solid friction), networks (biological networks, urban traffic, the internet, vulnerability of networks, optimal transport networks) and cellular automata applied to the various aforementioned fields.

  18. Car-following models of vehicular traffic

    Institute of Scientific and Technical Information of China (English)

    翁彦琳; 吴铁军

    2002-01-01

    The Car-following model is a kind of microscopic simulation model for vehicular traffic, which describe the one-by-one following behaviors of vehides in the same traffic lane. As a common traffic phenomenon, following behavior is of great importance in the micro-study of intelligent traffic control.Compared with other traffic-flow models, car-following model embodies the human factors and feflects the real traffic sit-uation in a better way. This paper gives a systematic review of the development and actuality of car-following models by introducing and analyzing in detail the advantages and disavantages of GHR model, OV model,CA model and fuzzy-logic model. In addition, local stability and asymptotic stability of car-following models are discussed in this paper.

  19. 11th Traffic and Granular Flow Conference

    CERN Document Server

    Daamen, Winnie

    2016-01-01

    The Conference on Traffic and Granular Flow brings together international researchers from different fields ranging from physics to computer science and engineering to discuss the latest developments in traffic-related systems. Originally conceived to facilitate new ideas by considering the similarities of traffic and granular flow, TGF'15, organised by Delft University of Technology, now covers a broad range of topics related to driven particle and transport systems. Besides the classical topics of granular flow and highway traffic, its scope includes data transport (Internet traffic), pedestrian and evacuation dynamics, intercellular transport, swarm behaviour and the collective dynamics of other biological systems. Recent advances in modelling, computer simulation and phenomenology are presented, and prospects for applications, for example to traffic control, are discussed. The conference explores the interrelations between the above-mentioned fields and offers the opportunity to stimulate interdisciplinar...

  20. Measures for Reducing Traffic Congestion in Cities

    Directory of Open Access Journals (Sweden)

    Ivan Dadić

    2012-10-01

    Full Text Available Traffic planners and designers are daily confronted by theproblems of traffic flow. Finding a solution for the optimal organisationof the traffic system in the wider (at the state leveland the narrower sense (cities, towns, requires accurate planningand adequate financing. With Croatia being a developingcountry that has higher investments into the traffic system, thematerial means need to be rati01~t~lised and the human andland resources cautiously managed. With the development andimplementation of the traffic studies, an improvement of thetraffic in cities can be expected. The work describes the trafficmeasures which provide higher quality traffic flow, with low investments.They can be used as guidelines in planning the trafficand as the first implementations of solutions from the trafficstudies.

  1. Car-following models of vehicular traffic

    Institute of Scientific and Technical Information of China (English)

    翁彦琳; 吴铁军

    2002-01-01

    The Car-following models is a kind of microscopic simulation model f or vehicular traffic, which describe the one-by-one following behaviors of v ehicles in the same traffic lane. As a common traffic phenomenon, following behavior is of great importance in the micro-study of intelligent traffic control. Compared with other traffic-flow models, car-following model embodies the human factors a nd reflects the real traffic situation in a better way. This paper gives a syste matic review of the development and actuality of car-following models by introd u cing and analyzing in detail the advantages and disadvantages of GHR model, OV m odel, CA model and fuzzy-logic model. In addition, local stability and asymptot ic stability of car-following models are discussed in this paper.

  2. Using Visualization Techniques in Multilayer Traffic Modeling

    Science.gov (United States)

    Bragg, Arnold

    We describe visualization techniques for multilayer traffic modeling - i.e., traffic models that span several protocol layers, and traffic models of protocols that cross layers. Multilayer traffic modeling is challenging, as one must deal with disparate traffic sources; control loops; the effects of network elements such as IP routers; cross-layer protocols; asymmetries in bandwidth, session lengths, and application behaviors; and an enormous number of complex interactions among the various factors. We illustrate by using visualization techniques to identify relationships, transformations, and scaling; to smooth simulation and measurement data; to examine boundary cases, subtle effects and interactions, and outliers; to fit models; and to compare models with others that have fewer parameters. Our experience suggests that visualization techniques can provide practitioners with extraordinary insight about complex multilayer traffic effects and interactions that are common in emerging next-generation networks.

  3. A System for Traffic Violation Detection

    Directory of Open Access Journals (Sweden)

    Nourdine Aliane

    2014-11-01

    Full Text Available This paper describes the framework and components of an experimental platform for an advanced driver assistance system (ADAS aimed at providing drivers with a feedback about traffic violations they have committed during their driving. The system is able to detect some specific traffic violations, record data associated to these faults in a local data-base, and also allow visualization of the spatial and temporal information of these traffic violations in a geographical map using the standard Google Earth tool. The test-bed is mainly composed of two parts: a computer vision subsystem for traffic sign detection and recognition which operates during both day and nighttime, and an event data recorder (EDR for recording data related to some specific traffic violations. The paper covers firstly the description of the hardware architecture and then presents the policies used for handling traffic violations.

  4. A System for Traffic Violation Detection

    Science.gov (United States)

    Aliane, Nourdine; Fernandez, Javier; Mata, Mario; Bemposta, Sergio

    2014-01-01

    This paper describes the framework and components of an experimental platform for an advanced driver assistance system (ADAS) aimed at providing drivers with a feedback about traffic violations they have committed during their driving. The system is able to detect some specific traffic violations, record data associated to these faults in a local data-base, and also allow visualization of the spatial and temporal information of these traffic violations in a geographical map using the standard Google Earth tool. The test-bed is mainly composed of two parts: a computer vision subsystem for traffic sign detection and recognition which operates during both day and nighttime, and an event data recorder (EDR) for recording data related to some specific traffic violations. The paper covers firstly the description of the hardware architecture and then presents the policies used for handling traffic violations. PMID:25421737

  5. Green Wave Traffic Optimization - A Survey

    DEFF Research Database (Denmark)

    Warberg, Andreas; Larsen, Jesper; Jørgensen, Rene Munk

    The objective of this survey is to cover the research in the area of adaptive traffic control with emphasis on the applied optimization methods. The problem of optimizing traffic signals can be viewed in various ways, depending on political, economic and ecological goals. The survey highlights some......-based, approach, which is shown to be more suitable for adaptive traffic control. To support this claim three adaptive systems, which use alternatives to the classical optimization procedures, are described in detail....

  6. Traffic forecasts under uncertainty and capacity constraints

    OpenAIRE

    2009-01-01

    Traffic forecasts provide essential input for the appraisal of transport investment projects. However, according to recent empirical evidence, long-term predictions are subject to high levels of uncertainty. This paper quantifies uncertainty in traffic forecasts for the tolled motorway network in Spain. Uncertainty is quantified in the form of a confidence interval for the traffic forecast that includes both model uncertainty and input uncertainty. We apply a stochastic simulation process bas...

  7. Empirical synchronized flow in oversaturated city traffic

    Science.gov (United States)

    Kerner, Boris S.; Hemmerle, Peter; Koller, Micha; Hermanns, Gerhard; Klenov, Sergey L.; Rehborn, Hubert; Schreckenberg, Michael

    2014-09-01

    Based on a study of anonymized GPS probe vehicle traces measured by personal navigation devices in vehicles randomly distributed in city traffic, empirical synchronized flow in oversaturated city traffic has been revealed. It turns out that real oversaturated city traffic resulting from speed breakdown in a city in most cases can be considered random spatiotemporal alternations between sequences of moving queues and synchronized flow patterns in which the moving queues do not occur.

  8. A Bathtub Model of Traffic Congestion

    OpenAIRE

    Arnott, Richard

    2011-01-01

    In the standard economic models of traffic congestion, traffic flow does not fall under heavily congested conditions. But this is counter to experience, especially in thedowntown areas of most major cities during rush hour. This paper presents a bathtub model of traffic congestion. The height of water in the bathtub corresponds totraffic density, velocity is negatively related to density, and outflow is the product of density and velocity. Above a critical density, outflow falls as density in...

  9. On the reproducibility of spatiotemporal traffic dynamics with microscopic traffic models

    CERN Document Server

    Knorr, Florian

    2012-01-01

    Traffic flow is a very prominent example of a driven non-equilibrium system. A characteristic phenomenon of traffic dynamics is the spontaneous and abrupt drop of the average velocity on a stretch of road leading to congestion. Such a traffic breakdown corresponds to a boundary-induced phase transition from free flow to congested traffic. In this paper, we study the ability of selected microscopic traffic models to reproduce a traffic breakdown, and we investigate its spatiotemporal dynamics. For our analysis, we use empirical traffic data from stationary loop detectors on a German Autobahn showing a spontaneous breakdown. We then present several methods to assess the results and compare the models with each other. In addition, we will also discuss some important modeling aspects and their impact on the resulting spatiotemporal pattern. The investigation of different downstream boundary conditions, for example, shows that the physical origin of the traffic breakdown may be artificially induced by the setup of...

  10. On the reproducibility of spatiotemporal traffic dynamics with microscopic traffic models

    Science.gov (United States)

    Knorr, Florian; Schreckenberg, Michael

    2012-10-01

    Traffic flow is a very prominent example of a driven non-equilibrium system. A characteristic phenomenon of traffic dynamics is the spontaneous and abrupt drop of the average velocity on a stretch of road leading to congestion. Such a traffic breakdown corresponds to a boundary-induced phase transition from free flow to congested traffic. In this paper, we study the ability of selected microscopic traffic models to reproduce a traffic breakdown, and we investigate its spatiotemporal dynamics. For our analysis, we use empirical traffic data from stationary loop detectors on a German Autobahn showing a spontaneous breakdown. We then present several methods to assess the results and compare the models with each other. In addition, we will also discuss some important modeling aspects and their impact on the resulting spatiotemporal pattern. The investigation of different downstream boundary conditions, for example, shows that the physical origin of the traffic breakdown may be artificially induced by the setup of the boundaries.

  11. Real-Time Corrected Traffic Correlation Model for Traffic Flow Forecasting

    Directory of Open Access Journals (Sweden)

    Hua-pu Lu

    2015-01-01

    Full Text Available This paper focuses on the problems of short-term traffic flow forecasting. The main goal is to put forward traffic correlation model and real-time correction algorithm for traffic flow forecasting. Traffic correlation model is established based on the temporal-spatial-historical correlation characteristic of traffic big data. In order to simplify the traffic correlation model, this paper presents correction coefficients optimization algorithm. Considering multistate characteristic of traffic big data, a dynamic part is added to traffic correlation model. Real-time correction algorithm based on Fuzzy Neural Network is presented to overcome the nonlinear mapping problems. A case study based on a real-world road network in Beijing, China, is implemented to test the efficiency and applicability of the proposed modeling methods.

  12. ATM traffic-policing and traffic-shaping measurements

    Science.gov (United States)

    Pugaczewski, Jack T.; Sanderson, Dave; Hoffman, David W.

    1997-10-01

    ATM (Asynchronous Transfer Mode) defines several Quality of Service (QoS) levels such as CBR (Constant Bit Rate) and VBR (Variable Bit Rate). Each service has parameters that are associated with its performance characteristic. The parameters used for specifying CBR service are Peak Cell Rate (PCR) and Cell Delay Variation Tolerance (CDVTOL). The parameters used for specifying VBR service are PCR, SCR (Sustainable Cell Rate), and BT (Burst Tolerance). The network takes responsibility for monitoring and policing the traffic of each VCC/VPC at the ingress of the network. The CPE needs to traffic shape at the ingress of the network in order to meet the contracted QoS requirements. Measurement procedures and tools are required in order to provide a means of tuning the CPE and network service to meet the customer's end-to-end QoS expectations. The following paper describes how the Generic Cell Rate Algorithm variables can be adjusted to provide acceptable levels of QoS in a mixed VBR, and CBR environment. In addition, the paper examines a methodology for measuring the performance of VBR and CBR services. The authors uses this information to build a QoS measurement tool.

  13. Air traffic management evaluation tool

    Science.gov (United States)

    Sridhar, Banavar (Inventor); Sheth, Kapil S. (Inventor); Chatterji, Gano Broto (Inventor); Bilimoria, Karl D. (Inventor); Grabbe, Shon (Inventor); Schipper, John F. (Inventor)

    2012-01-01

    Methods for evaluating and implementing air traffic management tools and approaches for managing and avoiding an air traffic incident before the incident occurs. A first system receives parameters for flight plan configurations (e.g., initial fuel carried, flight route, flight route segments followed, flight altitude for a given flight route segment, aircraft velocity for each flight route segment, flight route ascent rate, flight route descent route, flight departure site, flight departure time, flight arrival time, flight destination site and/or alternate flight destination site), flight plan schedule, expected weather along each flight route segment, aircraft specifics, airspace (altitude) bounds for each flight route segment, navigational aids available. The invention provides flight plan routing and direct routing or wind optimal routing, using great circle navigation and spherical Earth geometry. The invention provides for aircraft dynamics effects, such as wind effects at each altitude, altitude changes, airspeed changes and aircraft turns to provide predictions of aircraft trajectory (and, optionally, aircraft fuel use). A second system provides several aviation applications using the first system. Several classes of potential incidents are analyzed and averted, by appropriate change en route of one or more parameters in the flight plan configuration, as provided by a conflict detection and resolution module and/or traffic flow management modules. These applications include conflict detection and resolution, miles-in trail or minutes-in-trail aircraft separation, flight arrival management, flight re-routing, weather prediction and analysis and interpolation of weather variables based upon sparse measurements. The invention combines these features to provide an aircraft monitoring system and an aircraft user system that interact and negotiate changes with each other.

  14. Robust Traffic Flow Management: Coevolutionary Approach Project

    Data.gov (United States)

    National Aeronautics and Space Administration — We will develop a Coevolutionary Decision Support Tool (CDST) that explicitly incorporates weather uncertainty (non-probabilistically) into strategic Traffic Flow...

  15. Urban Traffic Information Service Application Grid

    Institute of Scientific and Technical Information of China (English)

    Chang-Jun Jiang; Yang Luo; An-Qing Zhou; Lin Chen; Lei Liu; Zhao-Hui Zhang; Guo-Sun Zeng; Hong-Zhong Chen; Duo-Qian Miao; Yu Fang; Xiao-Feng Tao; Qing Zhi; Feng Yue

    2005-01-01

    Traffic information processing is very complicated because of dynamic, cooperative and distributed features. This paper describes the prototype system version 2.0 of Urban Traffic Information Service Application Grid (UTISAG),which is based on the previous version. In this version, a new architecture and more enhanced services are introduced.The remarkable characteristic of the new system is providing dynamic information services for travelers by grid technology. Therefore, the key research includes integrating large multi-source traffic data, forecasting route status, simulating regional traffic flow parallelly, and implementing optimum dynamic travel scheme based on massive GPS data.

  16. Synchronized flow in oversaturated city traffic

    Science.gov (United States)

    Kerner, Boris S.; Klenov, Sergey L.; Hermanns, Gerhard; Hemmerle, Peter; Rehborn, Hubert; Schreckenberg, Michael

    2013-11-01

    Based on numerical simulations with a stochastic three-phase traffic flow model, we reveal that moving queues (moving jams) in oversaturated city traffic dissolve at some distance upstream of the traffic signal while transforming into synchronized flow. It is found that, as in highway traffic [Kerner, Phys. Rev. EPLEEE81539-375510.1103/PhysRevE.85.036110 85, 036110 (2012)], such a jam-absorption effect in city traffic is explained by a strong driver's speed adaptation: Time headways (space gaps) between vehicles increase upstream of a moving queue (moving jam), resulting in moving queue dissolution. It turns out that at given traffic signal parameters, the stronger the speed adaptation effect, the shorter the mean distance between the signal location and the road location at which moving queues dissolve fully and oversaturated traffic consists of synchronized flow only. A comparison of the synchronized flow in city traffic found in this Brief Report with synchronized flow in highway traffic is made.

  17. Traffic Flow Characteristics with Transportation Plans Components

    Directory of Open Access Journals (Sweden)

    Hrvoje Baričević

    2012-10-01

    Full Text Available Knowledge of traffic characteristics is useful to the highwayengineer in developing highway and transportation plans, performingeconomic analyses, establishing geometric design criteria,selecting and implementing traffic control measures, andevaluating the pe1[ormance of transportation facilities. Doze/ISof measures have been employed to describe the quality andquantity of traffic flow. This paper presents information onthose flow characteristics that fundamentally bear on the planning,design, and operation of highway and transport facilities:traffic speed, travel time, volume, and density.

  18. Critical density of urban traffic

    CERN Document Server

    da Silva, Adilton Jose

    2010-01-01

    A modified version of the Intelligent Driver Model was used to simulate traffic in the district of Afogados, in the city of Recife, Brazil, with the objective to verify whether the complexity of the underlying street grid, with multiple lane streets, crossings, and semaphores, is capable of exhibiting the effect of critical density: appearance of a maximum in the vehicle flux versus density curve. Numerical simulations demonstrate that this effect indeed is observed on individual avenues, while the phase offset among the avenues results in damping of this effect for the region as a whole.

  19. Bits of Internet traffic control

    OpenAIRE

    Vojnovic, Milan; Le Boudec, Jean Yves

    2005-01-01

    In this work, we consider four problems in the context of Internet traffic control. The first problem is to understand when and why a sender that implements an equation-based rate control would be TCP-friendly, or not—a sender is said to be TCP-friendly if, under the same operating conditions, its long-term average send rate does not exceed that of a TCP sender. It is an established axiom that some senders in the Internet would need to be TCP-friendly. An equation-based rate control sender pl...

  20. Bits of Internet Traffic Control

    OpenAIRE

    Vojnovic, Milan

    2003-01-01

    In this work, we consider four problems in the context of Internet traffic control. The first problem is to understand when and why a sender that implements an equation-based rate control would be TCP-friendly, or not—a sender is said to be TCP-friendly if, under the same operating conditions, its long-term average send rate does not exceed that of a TCP sender. It is an established axiom that some senders in the Internet would need to be TCP-friendly. An equation-based rate control sender pl...

  1. Sociological study on the course of the traffic system and the traffic accidents

    OpenAIRE

    Yaşar Erjem

    2007-01-01

    The subject of this research is the operation of the traffic system and traffic accidents from a sociological perspective. In the research , the traffic system is taken as a sub-system of the social system. In this context the effects of the social, cultural and demographic factors on traffic accidents are examined . The data were collected from 681 people of whom 300 are drivers 300 pedestrians, 4 directors and 20 instructors of driving courses in Konya in Turkey. The research findings showe...

  2. 32 CFR 634.24 - Traffic planning and codes.

    Science.gov (United States)

    2010-07-01

    ..., and points of congestion. The installation law enforcement officer and traffic engineer usually... 32 National Defense 4 2010-07-01 2010-07-01 true Traffic planning and codes. 634.24 Section 634.24... CRIMINAL INVESTIGATIONS MOTOR VEHICLE TRAFFIC SUPERVISION Traffic Supervision § 634.24 Traffic planning...

  3. MPLS & Internet Traffic Engineering%MPLS和Internet流量工程

    Institute of Scientific and Technical Information of China (English)

    孟魁; 张根度

    2001-01-01

    Rapid growth of Internet traffic and increasing requirement for service quality have made traffic engineering an essential consideration in the operation of large Internet backbone networks. Historically,traffic engineering in the Internet has been hampered by the conventional IP technologies. This article discusses the challenge of Internet Traffic Engineering and the applications of MPLS to traffic engineering in IP networks.

  4. 14 CFR 93.123 - High density traffic airports.

    Science.gov (United States)

    2010-01-01

    ... 14 Aeronautics and Space 2 2010-01-01 2010-01-01 false High density traffic airports. 93.123... (CONTINUED) AIR TRAFFIC AND GENERAL OPERATING RULES SPECIAL AIR TRAFFIC RULES High Density Traffic Airports § 93.123 High density traffic airports. (a) Each of the following airports is designated as a...

  5. Block copolymer battery separator

    Science.gov (United States)

    Wong, David; Balsara, Nitash Pervez

    2016-04-26

    The invention herein described is the use of a block copolymer/homopolymer blend for creating nanoporous materials for transport applications. Specifically, this is demonstrated by using the block copolymer poly(styrene-block-ethylene-block-styrene) (SES) and blending it with homopolymer polystyrene (PS). After blending the polymers, a film is cast, and the film is submerged in tetrahydrofuran, which removes the PS. This creates a nanoporous polymer film, whereby the holes are lined with PS. Control of morphology of the system is achieved by manipulating the amount of PS added and the relative size of the PS added. The porous nature of these films was demonstrated by measuring the ionic conductivity in a traditional battery electrolyte, 1M LiPF.sub.6 in EC/DEC (1:1 v/v) using AC impedance spectroscopy and comparing these results to commercially available battery separators.

  6. Hawaii Census 2000 Blocks

    Data.gov (United States)

    U.S. Environmental Protection Agency — This data layer represents Census 2000 demographic data derived from the PL94-171 redistricting files and SF3. Census geographic entities include blocks, blockgroups...

  7. Steroidal neuromuscular blocking agents

    NARCIS (Netherlands)

    Wierda, JMKH; Mori, K; Ohmura, A; Toyooka, H; Hatano, Y; Shingu, K; Fukuda, K

    1998-01-01

    Since 1964 approximately 20 steroidal neuromuscular blocking agents have been evaluated clinically. Pancuronium, a bisquaternary compound designed on the drawingboard, was the first steroidal relaxant introduced into clinical practice worldwide in the 1970's. Although a major improvement, pancuroniu

  8. Superalloy Lattice Block Structures

    Science.gov (United States)

    Nathal, M. V.; Whittenberger, J. D.; Hebsur, M. G.; Kantzos, P. T.; Krause, D. L.

    2004-01-01

    Initial investigations of investment cast superalloy lattice block suggest that this technology will yield a low cost approach to utilize the high temperature strength and environmental resistance of superalloys in lightweight, damage tolerant structural configurations. Work to date has demonstrated that relatively large superalloy lattice block panels can be successfully investment cast from both IN-718 and Mar-M247. These castings exhibited mechanical properties consistent with the strength of the same superalloys measured from more conventional castings. The lattice block structure also accommodates significant deformation without failure, and is defect tolerant in fatigue. The potential of lattice block structures opens new opportunities for the use of superalloys in future generations of aircraft applications that demand strength and environmental resistance at elevated temperatures along with low weight.

  9. Analysis of single-hop traffic grooming in mesh WDM optical networks

    Science.gov (United States)

    Xin, Chunsheng; Qiao, Chunming; Dixit, Sudhir

    2003-10-01

    Traffic grooming is a significant task in internetworking between an optical wavelength-routed core network that supplies "pipes" at the wavelength-granularity, and the attached client (e.g., IP) networks that usually require connections of sub-wavelength granularity. The focus of this study is to develop a theoretical performance analysis model for online traffic grooming in mesh optical networks. This paper first briefly discusses the difficulty in applying the analytic models developed for circuit-switched networks (including wavelength-routed optical networks) to the traffic grooming problem. It then develops a link blocking model based on the continuous time Markov chain and queueing theory, and finally conducts end-to-end performance analysis based on the Erlang fixed-point approximation. The results obtained from the analytic model are shown to match well with numerical results obtained from simulations.

  10. Forecasting Multivariate Road Traffic Flows Using Bayesian Dynamic Graphical Models, Splines and Other Traffic Variables

    NARCIS (Netherlands)

    Anacleto, Osvaldo; Queen, Catriona; Albers, Casper J.

    2013-01-01

    Traffic flow data are routinely collected for many networks worldwide. These invariably large data sets can be used as part of a traffic management system, for which good traffic flow forecasting models are crucial. The linear multiregression dynamic model (LMDM) has been shown to be promising for f

  11. A Behavioural Model of Traffic Congestion: Endogenizing Speed Choice, Traffic Safety and Time Losses

    NARCIS (Netherlands)

    Verhoef, E.T.; Rouwendal, J.

    2004-01-01

    Conventional economic models of traffic congestion assume that the relation between traffic flow and speed is a technical one. This paper develops a behavioural model of traffic congestion, in which drivers optimize their speeds by trading off time costs, expected accident costs and fuel costs. Sinc

  12. THE DEVELOPMENT OF TRAFFIC AND TRAFFIC SAFETY IN SIX DEVELOPED COUNTRIES

    NARCIS (Netherlands)

    OPPE, S

    1991-01-01

    Two models are presented, describing the development of traffic and traffic safety. Traffic volumes, measured by the total amount ofvehicle kilometres per year, are expected to follow a sigmoid saturation curve over time. The logistic function is used to model this development. The fatality rate, th

  13. 77 FR 55439 - Vessel Traffic Service Updates, Including Establishment of Vessel Traffic Service Requirements...

    Science.gov (United States)

    2012-09-10

    ... rulemaking would change the entry for Port Arthur from ``Sabine Traffic'' to ``Port Arthur Traffic'' to more...--003669955: Port Arthur Traffic 156.050 MHz (Ch. 01A)...... The navigable waters of the Sabine-Neches Canal south of 29 52.7' N.; Port Arthur Canal; Sabine Pass Channel; Sabine Bank Channel; Sabine Outer...

  14. Development of River Traffic in the Traffic System of Central Croatia

    Directory of Open Access Journals (Sweden)

    Dragan Badanjak

    2006-05-01

    Full Text Available From the aspect of public transport of passengers andgoods, the traffic system in Central Croatia consists of road,rail, air and river subsystems. Regarding their share in the volumeof passenger and cargo transport as well as the carried outtransport operations in the traffic system of Central Croatia,road and rail traffic are the dominant traffic subsystems. Regardingits technical, technological, organizational and economicindicators, the river traffic system represents the less developedsubsystem of the traffic system of Central Croatia, andthe situation in this respect is not much different at the nationallevel either.The possibilities for the development of river traffic systemhave not been sufficiently used, although the potential trafficdemand for the transportation services are substantial, especiallywhen considering the river Sava which potentially offersa direct connection to the trunk European waterway. The advantagesof the Central Croatian location undoubtedly lie inthe traffic and geo-strategic position, but the value of this locationcan be used only then when the quality level of the trafficsystem and the construction level of the infrastructurecatch up with the ones in the developed European countries.The conditions for the traffic development are first of allstrong and daily faster changes in the strata, especially regardingthe implementation of new solutions and increasing theefficiency of the overall traffic system. It is precisely for thisreason that special interest should be to study the traffic valuesaccording to the available data, establish their possiblerules as function of time, and design appropriate mathematicalmodels with the aim of forecasting the traffic developmentin the future.

  15. Urban traffic noise and the relation to urban density, form, and traffic elasticity

    NARCIS (Netherlands)

    Salomons, E.M.; Berghauser Pont, M.

    2012-01-01

    Traffic noise in cities has serious effects on the inhabitants. Well-known effects are annoyance and sleep disturbance, but long-term health effects such as cardiovascular disease have also been related to traffic noise. The spatial distribution of traffic noise in a city is related to the distribut

  16. Traffic Generator (TrafficGen) Version 1.4.2: Users Guide

    Science.gov (United States)

    2016-06-01

    Headquarters Services , Directorate for Information Operations and Reports (0704-0188), 1215 Jefferson Davis Highway, Suite 1204, Arlington, VA 22202...NOTES 14. ABSTRACT Network science experimentation often requires modeling of realistic network traffic specific to the experiment environment and...the network with Transmission Control Protocol and User Datagram Protocol Internet Protocol traffic. Each node generating network traffic in an

  17. Traffic Dynamics of Computer Networks

    CERN Document Server

    Fekete, Attila

    2008-01-01

    Two important aspects of the Internet, namely the properties of its topology and the characteristics of its data traffic, have attracted growing attention of the physics community. My thesis has considered problems of both aspects. First I studied the stochastic behavior of TCP, the primary algorithm governing traffic in the current Internet, in an elementary network scenario consisting of a standalone infinite-sized buffer and an access link. The effect of the fast recovery and fast retransmission (FR/FR) algorithms is also considered. I showed that my model can be extended further to involve the effect of link propagation delay, characteristic of WAN. I continued my thesis with the investigation of finite-sized semi-bottleneck buffers, where packets can be dropped not only at the link, but also at the buffer. I demonstrated that the behavior of the system depends only on a certain combination of the parameters. Moreover, an analytic formula was derived that gives the ratio of packet loss rate at the buffer ...

  18. Second-order phase transition in two-dimensional cellular automaton model of traffic flow containing road sections

    Science.gov (United States)

    Shi, Xiao-Qiu; Wu, Yi-Qi; Li, Hong; Zhong, Rui

    2007-11-01

    Two-dimensional cellular automaton model has been broadly researched for traffic flow, as it reveals the main characteristics of the traffic networks in cities. Based on the BML models, a first-order phase transition occurs between the low-density moving phase in which all cars move at maximal speed and the high-density jammed phase in which all cars are stopped. However, it is not a physical result of a realistic system. We propose a new traffic rule in a two-dimensional traffic flow model containing road sections, which reflects that a car cannot enter into a road crossing if the road section in front of the crossing is occupied by another car. The simulation results reveal a second-order phase transition that separates the free flow phase from the jammed phase. In this way the system will not be entirely jammed (“don’t block the box” as in New York City).

  19. Respiratory symptoms in children living near busy roads and their relationship to vehicular traffic: results of an Italian multicenter study (SIDRIA 2

    Directory of Open Access Journals (Sweden)

    Dell'Orco Valerio

    2009-06-01

    Full Text Available Abstract Background Epidemiological studies have provided evidence that exposure to vehicular traffic increases the prevalence of respiratory symptoms and may exacerbate pre-existing asthma in children. Self-reported exposure to road traffic has been questioned as a reliable measurement of exposure to air pollutants. The aim of this study was to investigate whether there were specific effects of cars and trucks traffic on current asthma symptoms (i.e. wheezing and cough or phlegm, and to examine the validity of self-reported traffic exposure. Methods The survey was conducted in 2002 in 12 centers in Northern, Center and Southern Italy, different in size, climate, latitude and level of urbanization. Standardized questionnaires filled in by parents were used to collect information on health outcomes and exposure to traffic among 33,632 6–7 and 13–14 years old children and adolescents. Three questions on traffic exposure were asked: the traffic in the zone of residence, the frequency of truck and of car traffic in the street of residence. The presence of a possible response bias for the self-reported traffic was evaluated using external validation (comparison with measurements of traffic flow in the city of Turin and internal validations (matching by census block, in the cities of Turin, Milan and Rome. Results Overall traffic density was weakly associated with asthma symptoms but there was a stronger association with cough or phlegm (high traffic density OR = 1.24; 95% CI: 1.04, 1.49. Car and truck traffic were independently associated with cough or phlegm. The results of the external validation did not support the existence of a reporting bias for the observed associations, for all the self-reported traffic indicators examined. The internal validations showed that the observed association between traffic density in the zone of residence and respiratory symptoms did not appear to be explained by an over reporting of traffic by parents of

  20. Oxidation by neutrophils-derived HOCl increases immunogenicity of proteins by converting them into ligands of several endocytic receptors involved in antigen uptake by dendritic cells and macrophages.

    Directory of Open Access Journals (Sweden)

    Rafał Biedroń

    Full Text Available The initiation of adaptive immune responses to protein antigens has to be preceded by their uptake by antigen presenting cells and intracellular proteolytic processing. Paradoxically, endocytic receptors involved in antigen uptake do not bind the majority of proteins, which may be the main reason why purified proteins stimulate at most weak immune responses. A shared feature of different types of adjuvants, capable of boosting immunogenicity of protein vaccines, is their ability to induce acute inflammation, characterized by early influx of activated neutrophils. Neutrophils are also rapidly recruited to sites of tissue injury or infection. These cells are the source of potent oxidants, including hypochlorous acid (HOCl, causing oxidation of proteins present in inflammatory foci. We demonstrate that oxidation of proteins by endogenous, neutrophils-derived HOCl increases their immunogenicity. Upon oxidation, different, randomly chosen simple proteins (yeast alcohol dehydrogenase, human and bovine serum albumin and glycoproteins (human apo-transferrin, ovalbumin gain the ability to bind with high affinity to several endocytic receptors on antigen presenting cells, which seems to be the major mechanism of their increased immunogenicity. The mannose receptor (CD206, scavenger receptors A (CD204 and CD36 were responsible for the uptake and presentation of HOCl-modified proteins by murine dendritic cells and macrophages. Other scavenger receptors, SREC-I and LOX-1, as well as RAGE were also able to bind HOCl-modified proteins, but they did not contribute significantly to these ligands uptake by dendritic cells because they were either not expressed or exhibited preference for more heavily oxidised proteins. Our results indicate that oxidation by neutrophils-derived HOCl may be a physiological mechanism of conferring immunogenicity on proteins which in their native forms do not bind to endocytic receptors. This mechanism might enable the immune system

  1. Modeling and Simulation for Urban Rail Traffic Problem Based on Cellular Automata

    Institute of Scientific and Technical Information of China (English)

    许琰; 曹成铉; 李明华; 罗金龙

    2012-01-01

    Based on the Nagel-Schreckenberg model, we propose a new cellular automata model to simulate the urban rail traffic flow under moving block system and present a new minimum instantaneous distance formula under pure moving block. We also analyze the characteristics of the urban rail traffic flow under the influence of train density, station dwell times, the length of train, and the train velocity. Train delays can be decreased effectively through flexible departure intervals according to the preceding train type before its departure. The results demonstrate that a suitable adjustment of the current train velocity based on the following train velocity can greatly shorten the minimum departure intervals and then increase the capacity of rail transit.

  2. Traffic Modelling for Capacity Analysis of CDMA Networks using Gausian Approximation Method

    Directory of Open Access Journals (Sweden)

    B. O. Omijeh

    2014-05-01

    Full Text Available This paper presents, modelling telephone traffic in cellular networks operating with Code Division Multiple Access (CDMA for the purpose of capacity analysis of such systems. With the current use of CDMA as the preferred multiple access technique due to its capacity advantage, there is the need for tools that will assist in ensuring quality of service and proper network dimensioning. This work produces a model useful for capacity analysis. Focusing on the reverse link, this is achieved by modelling telephone traffic using Gaussian assumptions to generate a CDMA blocking probability that is adapted into Erlang B formula for capacity calculations. A program written in MATLAB is used to realise the blocking probability formula with graphical outputs that is a tool for dimensioning. Results show that variations in network parameters affect CDMA capacity and that CDMA has a huge capacity advantage over TDMA and FDMA

  3. Calculating Traffic based on Road Sensor Data

    NARCIS (Netherlands)

    Bisseling, Rob; Gao, Fengnan; Hafkenscheid, Patrick; Idema, Reijer; Jetka, Tomasz; Guerra Ones, Valia; Sikora, Monika

    2014-01-01

    Road sensors gather a lot of statistical data about traffic. In this paper, we discuss how a measure for the amount of traffic on the roads can be derived from this data, such that the measure is independent of the number and placement of sensors, and the calculations can be performed quickly for la

  4. Beijing Grapples with1 Traffic Jams2

    Institute of Scientific and Technical Information of China (English)

    慎益

    2004-01-01

    Traffice congestion3 is a prominent4 problem as the number of private cars is soaring5 by more than 1,000 each day in Beijing. In 2003, Beijing recorded about 2000 tragic jams. Traffic management officials complained that "rush hour6" now occupies 12 hours of the day. The city

  5. Formation and Propagation of Local Traffic Jam

    Directory of Open Access Journals (Sweden)

    Hong-sheng Qi

    2013-01-01

    Full Text Available Large scale traffic congestion often stems from local traffic jam in single road or intersection. In this paper, macroscopic method was used to explore the formation and propagation of local traffic jam. It is found that (1 the propagation of traffic jam can be seen as the propagation of traffic signal parameters, that is, virtual split and virtual green time; (2 for a road with endogenous flow, entrance location influences the jam propagation. With the same demand (upstream links flow and entrance flow, the upstream got more influence; (3 when a one-lane road is thoroughly congested, virtual signal parameters everywhere are the same as that at stop line; for a basic road, the virtual signals work in a cooperative manner; (4 phase sequence is one important parameter that influences traffic performances during peak hour where spill back of channelization takes place. The same phase plan for left-turn flow and through flow would be preferred; (5 signal coordination plays an important role in traffic jam propagation and hence effective network signal parameters should be designed to prevent jam from propagation to the whole network. These findings would serve as a basis for future network traffic congestion control.

  6. An analysis of the traffic safety phenomenon.

    NARCIS (Netherlands)

    Asmussen, E. & Kranenburg, A.

    1982-01-01

    The lack of traffic safety is a combination of the critical coincidence of circumstances in the traffic of incidents (near-accidents) and accidents with unwanted (permanent) consequences, such as fatalities, injured and disabled persons and material damage. This definition covers the whole of the cr

  7. Consistency Analysis of Network Traffic Repositories

    NARCIS (Netherlands)

    Lastdrager, Elmer; Pras, Aiko

    2009-01-01

    Traffic repositories with TCP/IP header information are very important for network analysis. Researchers often assume that such repositories reliably represent all traffic that has been flowing over the network; little thoughts are made regarding the consistency of these repositories. Still, for var

  8. SIMPLE LATTICE BOLTZMANN MODEL FOR TRAFFIC FLOWS

    Institute of Scientific and Technical Information of China (English)

    Yan Guangwu; Hu Shouxin

    2000-01-01

    A lattice Boltzmann model with 5-bit lattice for traffic flows is proposed.Using the Chapman-Enskog expansion and multi-scale technique,we obtain the higher-order moments of equilibrium distribution function.A simple traffic light problem is simulated by using the present lattice Boltzmann model,and the result agrees well with analytical solution.

  9. On traffic modelling in GPRS networks

    DEFF Research Database (Denmark)

    Madsen, Tatiana Kozlova; Schwefel, Hans-Peter; Prasad, Ramjee

    2005-01-01

    here are done at the Gi interface. The aim of this paper is to reveal some key statistics of GPRS data applications and to validate if the existing traffic models can adequately describe traffic volume and inter-arrival time distribution for different services. Additionally, we present a method of user...

  10. Traffic Light Detection Using Conic Section Geometry

    Science.gov (United States)

    Hosseinyalmdary, S.; Yilmaz, A.

    2016-06-01

    Traffic lights detection and their state recognition is a crucial task that autonomous vehicles must reliably fulfill. Despite scientific endeavors, it still is an open problem due to the variations of traffic lights and their perception in image form. Unlike previous studies, this paper investigates the use of inaccurate and publicly available GIS databases such as OpenStreetMap. In addition, we are the first to exploit conic section geometry to improve the shape cue of the traffic lights in images. Conic section also enables us to estimate the pose of the traffic lights with respect to the camera. Our approach can detect multiple traffic lights in the scene, it also is able to detect the traffic lights in the absence of prior knowledge, and detect the traffics lights as far as 70 meters. The proposed approach has been evaluated for different scenarios and the results show that the use of stereo cameras significantly improves the accuracy of the traffic lights detection and pose estimation.

  11. Dynamic traffic assignment on parallel computers

    Energy Technology Data Exchange (ETDEWEB)

    Nagel, K.; Frye, R.; Jakob, R.; Rickert, M.; Stretz, P.

    1998-12-01

    The authors describe part of the current framework of the TRANSIMS traffic research project at the Los Alamos National Laboratory. It includes parallel implementations of a route planner and a microscopic traffic simulation model. They present performance figures and results of an offline load-balancing scheme used in one of the iterative re-planning runs required for dynamic route assignment.

  12. Survivable Impairment-Aware Traffic Grooming

    NARCIS (Netherlands)

    Beshir, A.; Nuijts, R.; Malhotra, R.; Kuipers, F.

    2011-01-01

    Traffic grooming allows efficient utilization of network capacity by aggregating several independent traffic streams into a wavelength. In addition, survivability and impairment-awareness (i.e., taking into account the effect of physical impairments) are two important issues that have gained a lot o

  13. GPS queues with heterogeneous traffic classes

    NARCIS (Netherlands)

    Borst, Sem; Mandjes, Michel; Uitert, van Miranda

    2002-01-01

    We consider a queue fed by a mixture of light-tailed and heavy-tailed traffic. The two traffic classes are served in accordance with the generalized processor sharing (GPS) discipline. GPS-based scheduling algorithms, such as weighted fair queueing (WFQ), have emerged as an important mechanism for a

  14. Road traffic noise and incident myocardial infarction

    DEFF Research Database (Denmark)

    Sørensen, Mette; Andersen, Zorana Jovanovic; Nordsborg, Rikke B;

    2012-01-01

    Both road traffic noise and ambient air pollution have been associated with risk for ischemic heart disease, but only few inconsistent studies include both exposures.......Both road traffic noise and ambient air pollution have been associated with risk for ischemic heart disease, but only few inconsistent studies include both exposures....

  15. Floating car data for traffic monitoring

    DEFF Research Database (Denmark)

    Torp, Kristian; Lahrmann, Harry Spaabæk

    2005-01-01

    This paper describes a complete prototype system that uses Floating Car Data (FCD) for both automatic and manual detection of queues in traffic. The system is developed under EU’s Tempo program. The systems consists of small hardware units placed in mobile traffic report units (we use taxis...

  16. Analysis of urban traffic patterns using clustering

    NARCIS (Netherlands)

    Weijermars, Wilhelmina Adriana Maria

    2007-01-01

    Mobility is still increasing, as are its corresponding negative side effects such as congestion and air pollution. To be able to take adequate measures to minimize these side effects, it is important to obtain insight into the functioning of the traffic system. In common practice, the traffic analys

  17. Road traffic emission monitoring on motorways

    NARCIS (Netherlands)

    Dittrich, M.G.; Beek, P.J.G. van

    2006-01-01

    Road traffic noise is one of the major environmental noise sources that is known to cause health problems. Despite existing noise reception items, the continuing growth of traffic causes higher levels than the lims allow. Consequently, excessive costs are incurred for noise barriers along roads and

  18. Transumo: Toward sustainable and integrated traffic management

    NARCIS (Netherlands)

    Arem, B. van; Pommer, J.

    2009-01-01

    The current and expected increase of traffic demand can not be matched with expansion of road infrastructure. Nor will traditional traffic management measures suffice in the long run. As a consequence we are faced with increasing congestion, an unreliable road network as well as safety and environme

  19. Highway Traffic Safety Manpower Functions Guide.

    Science.gov (United States)

    Daugherty, Ronald D.; And Others

    The purpose of the project, "Revision and Update of Traffic Safety Manpower Training Program Development Guide," was to develop the HIGHWAY TRAFFIC SAFETY MANPOWER FUNCTIONS GUIDE. This document provides an organizational schema illustrating the functions essential to be performed and the interrelationship of these functions to carry out…

  20. Traffic states and jamming transitions induced by a bus in two-lane traffic flow

    Science.gov (United States)

    Nagai, Ryoichi; Nagatani, Takashi; Taniguchi, Naoki

    2005-05-01

    We study the traffic states and jamming transitions induced by a bus (slow car) in a two-lane traffic of cars. We use the dynamic model which is an extended one of the optimal velocity model to take into account the lane changing. The fundamental (flow-density) diagram is presented. The fundamental diagram changes highly by introducing a bus on a two-lane roadway. It is found that there are the six distinct states for the two-lane traffic flow including a bus. The spatio-temporal patterns are presented for the distinct traffic states. The dynamical state of traffic changes with density of cars. It is shown that the dynamical transitions among the distinct traffic states occur at some values of density. The phase diagram (region map) is shown for the two-lane traffic flow including a bus.

  1. Traffic congestion in interconnected complex networks.

    Science.gov (United States)

    Tan, Fei; Wu, Jiajing; Xia, Yongxiang; Tse, Chi K

    2014-06-01

    Traffic congestion in isolated complex networks has been investigated extensively over the last decade. Coupled network models have recently been developed to facilitate further understanding of real complex systems. Analysis of traffic congestion in coupled complex networks, however, is still relatively unexplored. In this paper, we try to explore the effect of interconnections on traffic congestion in interconnected Barabási-Albert scale-free networks. We find that assortative coupling can alleviate traffic congestion more readily than disassortative and random coupling when the node processing capacity is allocated based on node usage probability. Furthermore, the optimal coupling probability can be found for assortative coupling. However, three types of coupling preferences achieve similar traffic performance if all nodes share the same processing capacity. We analyze interconnected Internet autonomous-system-level graphs of South Korea and Japan and obtain similar results. Some practical suggestions are presented to optimize such real-world interconnected networks accordingly.

  2. Traffic flow dynamics data, models and simulation

    CERN Document Server

    Treiber, Martin

    2013-01-01

    This textbook provides a comprehensive and instructive coverage of vehicular traffic flow dynamics and modeling. It makes this fascinating interdisciplinary topic, which to date was only documented in parts by specialized monographs, accessible to a broad readership. Numerous figures and problems with solutions help the reader to quickly understand and practice the presented concepts. This book is targeted at students of physics and traffic engineering and, more generally, also at students and professionals in computer science, mathematics, and interdisciplinary topics. It also offers material for project work in programming and simulation at college and university level. The main part, after presenting different categories of traffic data, is devoted to a mathematical description of the dynamics of traffic flow, covering macroscopic models which describe traffic in terms of density, as well as microscopic many-particle models in which each particle corresponds to a vehicle and its driver. Focus chapters on ...

  3. A Traffic Restriction Scheme for Enhancing Carpooling

    Directory of Open Access Journals (Sweden)

    Dong Ding

    2017-01-01

    Full Text Available For the purpose of alleviating traffic congestion, this paper proposes a scheme to encourage travelers to carpool by traffic restriction. By a variational inequity we describe travelers’ mode (solo driving and carpooling and route choice under user equilibrium principle in the context of fixed demand and detect the performance of a simple network with various restriction links, restriction proportions, and carpooling costs. Then the optimal traffic restriction scheme aiming at minimal total travel cost is designed through a bilevel program and applied to a Sioux Fall network example with genetic algorithm. According to various requirements, optimal restriction regions and proportions for restricted automobiles are captured. From the results it is found that traffic restriction scheme is possible to enhance carpooling and alleviate congestion. However, higher carpooling demand is not always helpful to the whole network. The topology of network, OD demand, and carpooling cost are included in the factors influencing the performance of the traffic system.

  4. The Analysis of Rail Traffic Control Systems

    Directory of Open Access Journals (Sweden)

    Darius Gaidelis

    2011-04-01

    Full Text Available The systems used for rail traffic control are compared. Their advantages and disadvantages are analysed. The present and future development of rail traffic control systems in various countries is considered. The possibility of adapting these microprocessor-based traffic control systems to the conditions of Lithuania is analysed. For this purpose, the analysis of goods transported by Lithuanian railways is made, and the non-uniform amount of goods transported on different road sections is evaluated. The number of the delayed trains due to the failure of traffic control systems in recent years is determined, the cost of installing a new microprocessor-based traffic control system at the railway station, as well as its repay time and the demand for employees are calculated. Article in Lithuanian

  5. Intelligent Model for Traffic Safety Applications

    Directory of Open Access Journals (Sweden)

    C. Chellappan

    2012-01-01

    Full Text Available Problem statement: This study presents an analysis on road traffic system focused on the use of communications to detect dangerous vehicles on roads and highways and how it could be used to enhance driver safety. Approach: The intelligent traffic safety application model is based on all traffic flow theories developed in the last years, leading to reliable representations of road traffic, which is of major importance in achieving the attenuation of traffic problems. The model also includes the decision making process from the driver in accelerating, decelerating and changing lanes. Results: The individuality of each of these processes appears from the model parameters that are randomly generated from statistical distributions introduced as input parameters. Conclusion: This allows the integration of the individuality factor of the population elements yielding knowledge on various driving modes at wide variety of situations.

  6. [Clinical examinations for the traffic accident patients].

    Science.gov (United States)

    Hitosugi, Masahito

    2008-11-30

    Traffic accident is a leading cause of unintentional death and about six-thousands annually died in Japan. As about one-million of persons suffer from traffic injuries, most of them seek medical attention. Therefore, medical staffs have to find the injuries accurately and treat immediately. Furthermore, the cause of accident should also be considered; why the accident was occurred, human error of the driver? To solve these problems, clinical examinations were needed. Medical staffs have to understand the characteristics of the traffic injuries: severe and multiple blunt injuries, popular injuries can be estimated with considering the pattern of the accident. Because some of the accidents are occurred when the driver is under the influence of alcohol and other drugs, screening of these subjects should be performed. Because the public is largely unaware of the preventable nature of traffic injuries, in addition to diagnose and treat accurately, we medical staffs have to attend on the primary prevention of the traffic injuries.

  7. Traffic congestion in interconnected complex networks

    CERN Document Server

    Tan, Fei; Xia, Yongxiang; Tse, Chi K

    2014-01-01

    Traffic congestion in isolated complex networks has been investigated extensively over the last decade. Coupled network models have recently been developed to facilitate further understanding of real complex systems. Analysis of traffic congestion in coupled complex networks, however, is yet to come. In this paper, we try to explore the effect of interconnections on traffic congestion in interconnected BA scale-free networks. We find that assortative coupling can alleviate traffic congestion better than disassortative and random coupling when the node processing capacity is allocated based on node usage probability. Furthermore, the optimal coupling probability can be found for assortative coupling. However, three types of coupling preferences achieve similar traffic performance if all nodes share the same processing capacity. We analyze interconnected Internet AS-level graphs of Japan and South Korea and obtain similar results. Some practical suggestions are presented to optimize such real-world interconnect...

  8. Using Automated Planning for Traffic Signals Control

    Directory of Open Access Journals (Sweden)

    Matija Gulić

    2016-08-01

    Full Text Available Solving traffic congestions represents a high priority issue in many big cities. Traditional traffic control systems are mainly based on pre-programmed, reactive and local techniques. This paper presents an autonomic system that uses automated planning techniques instead. These techniques are easily configurable and modified, and can reason about the future implications of actions that change the default traffic lights behaviour. The proposed implemented system includes some autonomic properties, since it monitors the current traffic state, detects if the system is degrading its performance, sets up new sets of goals to be achieved by the planner, triggers the planner that generates plans with control actions, and executes the selected courses of actions. The obtained results in several artificial and real world data-based simulation scenarios show that the proposed system can efficiently solve traffic congestion.

  9. Distributed traffic signal control using fuzzy logic

    Science.gov (United States)

    Chiu, Stephen

    1992-01-01

    We present a distributed approach to traffic signal control, where the signal timing parameters at a given intersection are adjusted as functions of the local traffic condition and of the signal timing parameters at adjacent intersections. Thus, the signal timing parameters evolve dynamically using only local information to improve traffic flow. This distributed approach provides for a fault-tolerant, highly responsive traffic management system. The signal timing at an intersection is defined by three parameters: cycle time, phase split, and offset. We use fuzzy decision rules to adjust these three parameters based only on local information. The amount of change in the timing parameters during each cycle is limited to a small fraction of the current parameters to ensure smooth transition. We show the effectiveness of this method through simulation of the traffic flow in a network of controlled intersections.

  10. Modeling Network Traffic in Wavelet Domain

    Directory of Open Access Journals (Sweden)

    Sheng Ma

    2004-12-01

    Full Text Available This work discovers that although network traffic has the complicated short- and long-range temporal dependence, the corresponding wavelet coefficients are no longer long-range dependent. Therefore, a "short-range" dependent process can be used to model network traffic in the wavelet domain. Both independent and Markov models are investigated. Theoretical analysis shows that the independent wavelet model is sufficiently accurate in terms of the buffer overflow probability for Fractional Gaussian Noise traffic. Any model, which captures additional correlations in the wavelet domain, only improves the performance marginally. The independent wavelet model is then used as a unified approach to model network traffic including VBR MPEG video and Ethernet data. The computational complexity is O(N for developing such wavelet models and generating synthesized traffic of length N, which is among the lowest attained.

  11. Traffic and Granular Flow ’03

    CERN Document Server

    Luding, Stefan; Bovy, Piet; Schreckenberg, Michael; Wolf, Dietrich

    2005-01-01

    These proceedings are the fifth in the series Traffic and Granular Flow, and we hope they will be as useful a reference as their predecessors. Both the realistic modelling of granular media and traffic flow present important challenges at the borderline between physics and engineering, and enormous progress has been made since 1995, when this series started. Still the research on these topics is thriving, so that this book again contains many new results. Some highlights addressed at this conference were the influence of long range electric and magnetic forces and ambient fluids on granular media, new precise traffic measurements, and experiments on the complex decision making of drivers. No doubt the “hot topics” addressed in granular matter research have diverged from those in traffic since the days when the obvious analogies between traffic jams on highways and dissipative clustering in granular flow intrigued both c- munities alike. However, now just this diversity became a stimulating feature of the ...

  12. Systemic Approach to Traffic Evaluation of Mostar Airport

    Directory of Open Access Journals (Sweden)

    Eldo Raguž

    2005-11-01

    Full Text Available The attempt of this work is to systematically find solutionsfor Mostar Airport development through technical and technologicalharmonization of traffic processes undertaken in twoseparate organizations - airport and air traffic control and coordinationbetween other traffic branches. The work uses theindicators of traffic flows and tourist trends in the region, andtogether with the mentioned simulations it attempts to evaluatethe traffic potentials in the region by affecting the change in thecurrent negative traffic flows at Mostar Airport.

  13. Estimation of annual average daily traffic with optimal adjustment factors

    OpenAIRE

    Alonso Oreña, Borja; Moura Berodia, José Luis; Ibeas Portilla, Ángel; Romero Junquera, Juan Pablo

    2014-01-01

    This study aimed to estimate the annual average daily traffic in inter-urban networks determining the best correlation (affinity) between the short period traffic counts and permanent traffic counters. A bi-level optimisation problem is proposed in which an agent in an upper level prefixes the affinities between short period traffic counts and permanent traffic counters stations and looks to minimise the annual average daily traffic calculation error while, in a lower level, an origin–destina...

  14. Right bundle branch block

    DEFF Research Database (Denmark)

    Bussink, Barbara E; Holst, Anders Gaarsdal; Jespersen, Lasse;

    2013-01-01

    AimsTo determine the prevalence, predictors of newly acquired, and the prognostic value of right bundle branch block (RBBB) and incomplete RBBB (IRBBB) on a resting 12-lead electrocardiogram in men and women from the general population.Methods and resultsWe followed 18 441 participants included.......5%/2.3% in women, P Right bundle branch block was associated with significantly.......60-1.62). The presence of IRBBB was not associated with any adverse outcome.ConclusionIn this cohort study, RBBB and IRBBB were two to three times more common among men than women. Right bundle branch block was associated with increased cardiovascular risk and all-cause mortality, whereas IRBBB was not. Contrary...

  15. ALCOHOL RELATED TRAFFIC SAFETY LEGISLATION

    Directory of Open Access Journals (Sweden)

    E.B.R. DESAPRIYA

    2002-01-01

    Full Text Available There is a substantial amount of evidence from experimental studies to indicate that a variety of individual skills are impaired at blood alcohol concentrations (BACs well below 0.05%. Epidemiological studies indicate that the risk of a crash increases sharply for drivers with BACs below 0.05%. The correlation between drunk driving and the risk of traffic accidents has been established on the individual as well as the aggregate level. The BAC level legally permitted is a public policy decision by legislators, while scientists can present experimental and epidemiological evidence indicating the BAC level at which psychomotor skills deteriorate and accident probabilities increase. There is considerable epidemiological evidence to support the fact that the risk of alcohol impaired drivers being involved in traffic crashes rises with increasing BAC's. By contrast, the evidence on the BAC at which a driver should be regarded as committing an offence has been the subject of much debate and various legislative decisions. Historically, per se laws specify BAC levels which are a compromise figure intended to reflect both the point at which a driver becomes significantly more likely to be involved in an accident than a comparative driver with a zero BAC and that which is politically acceptable, but falls within the BAC region of increased accident liability. Therefore, the per se legislation in most countries has not kept pace with scientific progress. This study suggests that if saving lives on the road is an important issue, then, passing laws that incorporate scientific and epidemiological studies, is necessary.

  16. The Stability of Multi-modal Traffic Network

    Institute of Scientific and Technical Information of China (English)

    HAN Ling-Hui; Sun Hui-Jun; ZHU Cheng-Juan; WU Jian-Jun; JIA Bin

    2013-01-01

    There is an explicit and implicit assumption in multimodal traffic equilibrium models,that is,if the equilibrium exists,then it will also occur.The assumption is very idealized; in fact,it may be shown that the quite contrary could happen,because in multimodal traffic network,especially in mixed traffic conditions the interaction among traffic modes is asymmetric and the asymmetric interaction may result in the instability of traffic system.In this paper,to study the stability of multimodal traffic system,we respectively present the travel cost function in mixed traffic conditions and in traffic network with dedicated bus lanes.Based on a day-to-day dynamical model,we study the evolution of daily route choice of travelers in multimodal traffic network using 10000 random initial values for different cases.From the results of simulation,it can be concluded that the asymmetric interaction between the cars and buses in mixed traffic conditions can lead the traffic system to instability when traffic demand is larger.We also study the effect of travelers' perception error on the stability of multimodal traffic network.Although the larger perception error can alleviate the effect of interaction between cars and buses and improve the stability of traffic system in mixed traffic conditions,the traffic system also become instable when the traffic demand is larger than a number.For all cases simulated in this study,with the same parameters,traffic system with dedicated bus lane has better stability for traffic demand than that in mixed traffic conditions.We also find that the network with dedicated bus lane has higher portion of travelers by bus than it of mixed traffic network.So it can be concluded that building dedicated bus lane can improve the stability of traffic system and attract more travelers to choose bus reducing the traffic congestion.

  17. Binding of SEC11 indicates its role in SNARE recycling after vesicle fusion and identifies two pathways for vesicular traffic to the plasma membrane.

    Science.gov (United States)

    Karnik, Rucha; Zhang, Ben; Waghmare, Sakharam; Aderhold, Christin; Grefen, Christopher; Blatt, Michael R

    2015-03-01

    SNARE (soluble N-ethylmaleimide-sensitive factor attachment protein receptor) proteins drive vesicle fusion in all eukaryotes and contribute to homeostasis, pathogen defense, cell expansion, and growth in plants. Two homologous SNAREs, SYP121 (=SYR1/PEN1) and SYP122, dominate secretory traffic to the Arabidopsis thaliana plasma membrane. Although these proteins overlap functionally, differences between SYP121 and SYP122 have surfaced, suggesting that they mark two discrete pathways for vesicular traffic. The SNAREs share primary cognate partners, which has made separating their respective control mechanisms difficult. Here, we show that the regulatory protein SEC11 (=KEULE) binds selectively with SYP121 to affect secretory traffic mediated by this SNARE. SEC11 rescued traffic block by dominant-negative (inhibitory) fragments of both SNAREs, but only in plants expressing the native SYP121. Traffic and its rescue were sensitive to mutations affecting SEC11 interaction with the N terminus of SYP121. Furthermore, the domain of SEC11 that bound the SYP121 N terminus was itself able to block secretory traffic in the wild type and syp122 but not in syp121 mutant Arabidopsis. Thus, SEC11 binds and selectively regulates secretory traffic mediated by SYP121 and is important for recycling of the SNARE and its cognate partners.

  18. Contaminated soil concrete blocks

    NARCIS (Netherlands)

    Korte, de A.C.J.; Brouwers, H.J.H.; Limbachiya, Mukesh C.; Kew, Hsein Y.

    2009-01-01

    According to Dutch law the contaminated soil needs to be remediated or immobilised. The main focus in this article is the design of concrete blocks, containing contaminated soil, that are suitable for large production, financial feasible and meets all technical and environmental requirements. In ord

  19. Effects of Block Scheduling

    Directory of Open Access Journals (Sweden)

    William R. Veal

    1999-09-01

    Full Text Available This study examined the effects of a tri-schedule on the academic achievement of students in a high school. The tri-schedule consists of traditional, 4x4 block, and hybrid schedules running at the same time in the same high school. Effectiveness of the schedules was determined from the state mandated test of basic skills in reading, language, and mathematics. Students who were in a particular schedule their freshman year were tested at the beginning of their sophomore year. A statistical ANCOVA test was performed using the schedule types as independent variables and cognitive skill index and GPA as covariates. For reading and language, there was no statistically significant difference in test results. There was a statistical difference mathematics-computation. Block mathematics is an ideal format for obtaining more credits in mathematics, but the block format does little for mathematics achievement and conceptual understanding. The results have content specific implications for schools, administrations, and school boards who are considering block scheduling adoption.

  20. Concrete Block Pavements

    Science.gov (United States)

    1983-03-01

    1967, Cedergren 1974, Federal Highway .’,U .. V,47 -’":: 37 Administration 1980). Block pavements have essentially the same prob- lems with moisture...Vicksburg, Miss. Cedergren , H. R. 1974. Drainage of Highway and Airfield Pavements, John Wiley and Sons, New VOk. I Cement and Concrete Association

  1. Block That Pain!

    Science.gov (United States)

    ... along with the National Institute of Dental and Craniofacial Research (NIDCR) and the National Institute of General Medical Sciences (NIGMS). This finding shows that a specific combination of two molecules can block only pain-related neurons. It holds the promise of major ...

  2. Research on the Method of Traffic Organization and Optimization Based on Dynamic Traffic Flow Model

    Directory of Open Access Journals (Sweden)

    Shu-bin Li

    2017-01-01

    Full Text Available The modern transportation system is becoming sluggish by traffic jams, so much so that it can harm the economic and society in our country. One of the reasons is the surging vehicles day by day. Another reason is the shortage of the traffic supply seriously. But the most important reason is that the traffic organization and optimization hardly met the conditions of modern transport development. In this paper, the practical method of the traffic organization and optimization used in regional area is explored by the dynamic traffic network analysis method. Firstly, the operational states of the regional traffic network are obtained by simulation method based on the self-developed traffic simulation software DynaCHINA, in which the improved traffic flow simulation model was proposed in order to be more suitable for actual domestic urban transport situation. Then the appropriated optimization model and algorithm were proposed according to different optimized content and organization goals, and the traffic simulation processes more suitable to regional optimization were designed exactly. Finally, a regional network in Tai’an city was selected as an example. The simulation results show that the proposed method is effective and feasible. It can provide strong scientific and technological support for the traffic management department.

  3. Multi Agent system Randomized in Road Traffic

    Directory of Open Access Journals (Sweden)

    Gouiouez Mounir,

    2013-02-01

    Full Text Available In recent years, the rapid growth of road traffic density in theworld generates a rising request for tools that can be used toanalyze and control the traffic networks. Microscopic trafficsimulation[1,2,3] has proven to be one of the most useful toolsfor analysis of various traffic systems. Thus microscopic trafficsimulation has become an ever increasing field of research anddevelopment. In this paper we present a new model formicroscopic traffic simulation; Traditional traffic simulationmodels neglect some real-life factors that need to be considered,such as the effect of random distribution in the entry of lanehence ,this paper presents the development of a concept and anassociated architecture called the MAS2RT (Multi Agentsystem Randomized in Road Traffic, which is based on thecombination of Multi-Agent Systems (MAS [4,5], Poissondistribution in urban Traffic and a stochastic model [6,7] tocapture the randomness of individual agent. The secondcontribution of this paper is about the internal structure ofmobile agents[8], initially reacts according to the instructions ofthe Main agent (MA and in the case of a lack of dynamicinformation, the mobile agents take decisions based on theirexperiences accumulated during previous interactions.The obtained results illustrate that using the randomness in thereaction of agent enhanced greatly the performance ofsimulation.

  4. TRAFFIC LIGHT VIOLATION AMONG MOTORISTS IN MALAYSIA

    Directory of Open Access Journals (Sweden)

    S. KULANTHAYAN

    2007-01-01

    Full Text Available Road crashes in Malaysia in 2006 stood at 341,232. This resulted in 6,287 deaths among road users giving an index of 23.5 road fatalities per 100,000 inhabitants. One of the possible reasons for the high number of crashes and injuries is due to beating traffic lights. Thus there is a need to investigate this alarming problem. A cross-sectional study was conducted in Selangor, Malaysia to identify road traffic-light violations. Traffic light violations are believed to be rising and resulted in 136 motorist fatalities and 155 injuries in 2002. Near-miss incidents could be higher as they go unreported. This study was conducted through observations from 14 December 2005 until 22 January 2006. Four locations were chosen to represent Selangor: Kajang, Kelang, Utara Subang Jaya (USJ and Bangi. During this 5-week period a total of 3,471 vehicles were observed. Data were analyzed using SPSS version 13.0. A bivariate analysis (logistic regression was applied to determine any relationship between traffic light violation and five identified variables. The results showed traffic light violation has a relationship with all five factors: day (weekday or weekend, camera enforcement, type of vehicle (two-wheel vehicles or four-wheel vehicles, traffic light cycle time (long or short and type of traffic lights (timer or normal.

  5. Cellphone as a Traffic Guidance System

    Directory of Open Access Journals (Sweden)

    Abdallah Alashqur

    2012-07-01

    Full Text Available The worldwide proliferation of cellphones has created numerous opportunities for new and powerful applications that contribute towards improving the quality of life. Traffic problems due to traffic congestions, especially in cities with high population density, is one of the areas that need attention. GPS-enabled cellphones can contribute towards alleviating some of these traffic problems. A GPS-enabled cellphone can be a source of useful information pertaining to traffic congestions. The velocity and location information that are transmitted by GPSs on the road can be gathered and analyzed at run-time to determine traffic status. In addition, a cellphone can receive area maps with overplayed traffic information to help drivers select optimal routes for their trips. In this paper we describe a prototype system that enables passengers who are carrying cellphones to know, in advance, the best route that a taxi needs to take by providing them with distance information, taxi fare estimate, and traffic congestion status. The system can be of benefit to other types of users in addition to passengers wanting to ride a taxi.

  6. A First Look at Modern Enterprise Traffic

    Energy Technology Data Exchange (ETDEWEB)

    Pang, Ruoming; Mark Allman, Mark; Bennett, Mike; Lee, Jason; Paxson, Vern; Tierney, Brian

    2005-06-01

    While wide-area Internet traffic has been heavily studied for many years, the characteristics of traffic inside Internet enterprises remain almost wholly unexplored. Nearly all of the studies of enterprise traffic available in the literature are well over a decade old and focus on individual LANs rather than whole sites. In this paper we present a broad overview of internal enterprise traffic recorded at a medium-sized site. The packet traces span more than 100 hours, over which activity from a total of several thousand internal hosts appears. This wealth of data--which we are publicly releasing in anonymized form--spans a wide range of dimensions. While we cannot form general conclusions using data from a single site, and clearly this sort of data merits additional in-depth study in a number of ways, in this work we endeavor to characterize a number of the most salient aspects of the traffic. Our goal is to provide a first sense of ways in which modern enterprise traffic is similar to wide-area Internet traffic, and ways in which it is quite different.

  7. Traffic forecaster for MPLS multimedia data streams

    Science.gov (United States)

    Ambrose, Barry; Lin, Freddie

    2006-05-01

    Contemporary high performance data networks carry a wide range of multimedia services (voice, video, audio, text, sensor data, etc.) that require an outstanding Quality of Service (QoS) to provide performance guarantees in priority delivery, latency, bandwidth utilization, load balancing, etc. With the advent of recent Multi-Protocol Label Switching (MPLS) network standards, the QoS has made significant progress in performance to provide these performance guarantees. Right now, attention has turned to the task of managing these QoS networks more efficiently through the handling of network traffic. We have investigated a novel Network Traffic Forecasting Assisted QoS Planner technology that will provide constantly updated forecasts of data traffic and server loads to any application that needs this information. Using source models of voice, video and data traffic based on empirical studies of TCP/IP data traffic carried out by Paxson and Floyd in the 1990's, our studies have shown that the system may provide up to 43% bandwidth savings for MPLS data streams, by predicting future traffic flows and reserving network resources to accommodate the predicted traffic. The system additionally provides a means to submit bandwidth reservation requests for those applications that need assured service guarantees for data delivery. The technology essentially increases the efficiency and effectiveness of multimedia information and communication network infrastructure that supports multiple or adaptive QoS levels for multimedia data networking and information system applications.

  8. Targeted delivery of lipid antigen to macrophages via the CD169/sialoadhesin endocytic pathway induces robust invariant natural killer T cell activation

    Science.gov (United States)

    Kawasaki, Norihito; Vela, Jose Luis; Nycholat, Corwin M.; Rademacher, Christoph; Khurana, Archana; van Rooijen, Nico; Crocker, Paul R.; Kronenberg, Mitchell; Paulson, James C.

    2013-01-01

    Invariant natural killer T (iNKT) cells induce a protective immune response triggered by foreign glycolipid antigens bound to CD1d on antigen-presenting cells (APCs). A limitation of using glycolipid antigens to stimulate immune responses in human patients has been the inability to target them to the most effective APCs. Recent studies have implicated phagocytic CD169+ macrophages as major APCs in lymph nodes for priming iNKT cells in mice immunized with glycolipid antigen in particulate form. CD169 is known as sialoadhesin (Sn), a macrophage-specific adhesion and endocytic receptor of the siglec family that recognizes sialic acid containing glycans as ligands. We have recently developed liposomes decorated with glycan ligands for CD169/Sn suitable for targeted delivery to macrophages via CD169/Sn-mediated endocytosis. Here we show that targeted delivery of a lipid antigen to CD169+ macrophages in vivo results in robust iNKT cell activation in liver and spleen using nanogram amounts of antigen. Activation of iNKT cells is abrogated in Cd169−/− mice and is macrophage-dependent, demonstrating that targeting CD169+ macrophages is sufficient for systemic activation of iNKT cells. When pulsed with targeted liposomes, human monocyte–derived dendritic cells expressing CD169/Sn activated human iNKT cells, demonstrating the conservation of the CD169/Sn endocytic pathway capable of presenting lipid antigens to iNKT cells. PMID:23610394

  9. Cooperative behavior of molecular motors: Cargo transport and traffic phenomena

    Science.gov (United States)

    Lipowsky, Reinhard; Beeg, Janina; Dimova, Rumiana; Klumpp, Stefan; Müller, Melanie J. I.

    2010-01-01

    All eukaryotic cells including those of our own body contain complex transport systems based on molecular motors which walk along cytoskeletal filaments. These motors are rather small and make discrete mechanical steps with a step size of the order of 10 nm but are able to pull cargo particles over much larger distances, from micrometers up to meters. In vivo, the intracellular cargos include large membrane-bounded organelles, smaller vesicles, a subset of mRNAs, cytoskeletal filaments, and various protein building blocks, which are transported between different cell compartments. This cargo transport is usually performed by teams of motors. If all motors belong to the same molecular species, the cooperative action of the motors leads to uni-directional transport with a strongly increased run length and with a characteristic force dependence of the velocity distributions. If two antagonistic teams of motors pull on the same cargo particle, they perform a stochastic tug-of-war, which is characterized by a subtle force balance between the two motor teams and leads to several distinct patterns of bi-directional transport. So far, all experimental observations on bi-directional transport are consistent with such a tug-of-war. If many motors and/or cargo particles are transported along the filaments, one encounters various traffic phenomena. Depending on their mutual interactions and the compartment geometry, the motors form various spatio-temporal patterns such as traffic jams, and undergo nonequilibrium phase transitions between different patterns of transport.

  10. Internet Traffic Classification for Educational Institutions Using Machine Learning

    Directory of Open Access Journals (Sweden)

    Jaspreet Kaur

    2012-07-01

    Full Text Available In recent times machine learning algorithms are used for internet traffic classification. The infinite number of websites in the internet world can be classified into different categories in different ways. In educational institutions, these websites can be classified into two categories, educational websites and non-educational websites. Educational websites are used to acquire knowledge, to explore educational topics while the non-educational websites are used for entertainment and to keep in touch with people. In case of blocking these non-educational websites students use proxy websites to unblock them. Therefore, in educational institutes for the optimum use of network resources the use of non-educational and proxy websites should be banned. In this paper, we use five ML classifiers Naïve Bayes, RBF, C4.5, MLP and Bayes Net to classify the educational and non-educational websites. Results show that Bayes Net gives best performance in both full feature and reduced feature data sets for intended classification of internet traffic in terms of classification accuracy, recall and precision values as compared to other classifiers.

  11. Effective bandwidth guaranteed routing schemes for MPLS traffic engineering

    Science.gov (United States)

    Wang, Bin; Jain, Nidhi

    2001-07-01

    In this work, we present online algorithms for dynamic routing bandwidth guaranteed label switched paths (LSPs) where LSP set-up requests (in terms of a pair of ingress and egress routers as well as its bandwidth requirement) arrive one by one and there is no a priori knowledge regarding future LSP set-up requests. In addition, we consider rerouting of LSPs in this work. Rerouting of LSPs has not been well studied in previous work on LSP routing. The need of LSP rerouting arises in a number of ways: occurrence of faults (link and/or node failures), re-optimization of existing LSPs' routes to accommodate traffic fluctuation, requests with higher priorities, and so on. We formulate the bandwidth guaranteed LSP routing with rerouting capability as a multi-commodity flow problem. The solution to this problem is used as the benchmark for comparing other computationally less costly algorithms studied in this paper. Furthermore, to more efficiently utilize the network resources, we propose online routing algorithms which route bandwidth demands over multiple paths at the ingress router to satisfy the customer requests while providing better service survivability. Traffic splitting and distribution over the multiple paths are carefully handled using table-based hashing schemes while the order of packets within a flow is preserved. Preliminary simulations are conducted to show the performance of different design choices and the effectiveness of the rerouting and multi-path routing algorithms in terms of LSP set-up request rejection probability and bandwidth blocking probability.

  12. Traffic-driven SIR epidemic model on networks

    CERN Document Server

    Pu, Cunlai; Yang, Jian

    2015-01-01

    We propose a novel SIR epidemic model which is driven by the transmission of infection packets in networks. Specifically, infected nodes generate and deliver infection packets causing the spread of the epidemic, while recovered nodes block the delivery of infection packets, and this inhibits the epidemic spreading. The efficient routing protocol governed by a control parameter $\\alpha$ is used in the packet transmission. We obtain the maximum instantaneous population of infected nodes, the maximum population of ever infected nodes, as well as the corresponding optimal $\\alpha$ through simulation. We find that generally more balanced load distribution leads to more intense and wide spread of an epidemic in networks. Increasing either average node degree or homogeneity of degree distribution will facilitate epidemic spreading. When packet generation rate $\\rho$ is small, increasing $\\rho$ favors epidemic spreading. However, when $\\rho$ is large enough, traffic congestion appears which inhibits epidemic spreadin...

  13. International Workshop on Traffic and Granular Flow

    CERN Document Server

    Herrmann, Hans; Schreckenberg, Michael; Wolf, Dietrich; Social, Traffic and Granular Dynamics

    2000-01-01

    "Are there common phenomena and laws in the dynamic behavior of granular materials, traffic, and socio-economic systems?" The answers given at the international workshop "Traffic and Granular Flow '99" are presented in this volume. From a physical standpoint, all these systems can be treated as (self)-driven many-particle systems with strong fluctuations, showing multistability, phase transitions, non-linear waves, etc. The great interest in these systems is due to several unexpected new discoveries and their practical relevance for solving some fundamental problems of today's societies. This includes intelligent measures for traffic flow optimization and methods from "econophysics" for stabilizing (stock) markets.

  14. Indiana Criminal Justice Institute Traffic Safety Division

    OpenAIRE

    Klitzsch, Ryan

    2014-01-01

    Traffic safety is important for a local community’s protection, health, and economic well-being. There are a variety of options available to communities that can help improve traffic safety and meet needs which, ideally, should be based on data that is specific to the area. We will discuss where a variety of data can be found and used by everyone from community members to engineers in order to improve traffic safety. Some of the options that will be discussed in this presentation are grant op...

  15. A statistical approach to vehicular traffic

    CERN Document Server

    Freund, J A; Freund, Jan; Poschel, Thorsten

    1995-01-01

    A two--dimensional cellular automaton is introduced to model the flow and jamming of vehicular traffic in cities. Each site of the automaton represents a crossing where a finite number of cars can wait approaching the crossing from each of the four directions. The flow of cars obeys realistic traffic rules. We investigate the dependence of the average velocity of cars on the global traffic density. At a critical threshold for the density the average velocity reduces drastically caused by jamming. For the low density regime we provide analytical results which agree with the numerical results.

  16. Stationarity Testing of Accumulated Ethernet Traffic

    Directory of Open Access Journals (Sweden)

    Zhiping Lu

    2013-01-01

    Full Text Available We investigate the stationarity property of the accumulated Ethernet traffic series. We applied several widely used stationarity and unit root tests, such as Dickey-Fuller test and its augmented version, Phillips-Perron test, as well as the Kwiatkowski-Phillips-Schmidt-Shin test and some of its generalizations, to the assessment of the stationarity of the traffic traces at the different time scales. The quantitative results in this research provide evidence that when the time scale increases, the accumulated traffic series are more stationary.

  17. TRAFFIC LIGHT VIOLATION AMONG MOTORISTS IN MALAYSIA

    OpenAIRE

    S. KULANTHAYAN

    2007-01-01

    Road crashes in Malaysia in 2006 stood at 341,232. This resulted in 6,287 deaths among road users giving an index of 23.5 road fatalities per 100,000 inhabitants. One of the possible reasons for the high number of crashes and injuries is due to beating traffic lights. Thus there is a need to investigate this alarming problem. A cross-sectional study was conducted in Selangor, Malaysia to identify road traffic-light violations. Traffic light violations are believed to be rising and resulted in...

  18. Strategic Framework of Air Traffic Development

    Directory of Open Access Journals (Sweden)

    Sanja Steiner

    2008-05-01

    Full Text Available The paper elaborates the past development of air traffic inEurope from the aspect of its economic benefits and ecologicallack of sustainability of conventional technology. The mainguidelines of the strategic development are related to the implementationof the global ecological standard and actual reformationprocesses of the air traffic management system postulatedby A TM 2000+ Strategy and the "Single European Sky"standard. The paper studies the wider aspects of integrated trafficdevelopment at the regional level and the factors influencingfurther development of the European air traffic.

  19. Pro CSS for High Traffic Websites

    CERN Document Server

    Kennedy, A

    2011-01-01

    Although web standards-based websites can scale effectively - and basic CSS will give you basic results - there are considerations and obstacles that high traffic websites must face to keep your development and hosting costs to a minimum. There are many tips and tricks, as well as down-to-earth best practice information, to make sure that everything runs quickly and efficiently with the minimum amount of fuss or developer intervention. Targeted at "high traffic" websites - those receiving over 10,000 unique visitors a day - Pro CSS for High Traffic Websites gives you inside informati

  20. Controlling Disorder in Traffic Flow by Perturbation

    Institute of Scientific and Technical Information of China (English)

    LIKe-Ping; GAOZi-You; CHENTian-Lun

    2004-01-01

    We propose a new technique for controlling disorder in traffic system. A kind of control signal which can be considered as a perturbation has been designated at a given site (perturbation point) of the single-lane highway. When a vehicle passes the perturbation point at a time, the velocity of the vehicle will be changed at the next time by the perturbation. This technique is tested for the deterministic NaSch traffic model. The simulation results indicate that the traffic system can be transited from the disorder states to the order states, such as fixed-point, periodic motion, etc.

  1. Delay of Vehicle Motion in Traffic Dynamics

    CERN Document Server

    Bando, M; Nakanishi, K; Nakayama, A; Bando, Masako; Hasebe, Katsuya; Nakanishi, Ken; Nakayama, Akihiro

    1996-01-01

    We demonstrate that in Optimal Velocity Model (OVM) delay times of vehicles coming from the dynamical equation of motion of OVM almost explain the order of delay times observed in actual traffic flows without introducing explicit delay times. Delay times in various cases are estimated: the case of a leader vehicle and its follower, a queue of vehicles controlled by traffic lights and many-vehicle case of highway traffic flow. The remarkable result is that in most of the situation for which we can make a reasonable definition of a delay time, the obtained delay time is of order 1 second.

  2. Stochastic Modeling of Traffic Air Pollution

    DEFF Research Database (Denmark)

    Thoft-Christensen, Palle

    2014-01-01

    In this paper, modeling of traffic air pollution is discussed with special reference to infrastructures. A number of subjects related to health effects of air pollution and the different types of pollutants are briefly presented. A simple model for estimating the social cost of traffic related air...... and using simple Monte Carlo techniques to obtain a stochastic estimate of the costs of traffic air pollution for infrastructures....... pollution is derived. Several authors have published papers on this very complicated subject, but no stochastic modelling procedure have obtained general acceptance. The subject is discussed basis of a deterministic model. However, it is straightforward to modify this model to include uncertain parameters...

  3. Tabletop Traffic Jams: Modeling Traffic Jams using Self Propelled Particles

    Science.gov (United States)

    Yadav, Vikrant; Kudrolli, Arshad

    2015-03-01

    We model behavior of traffic using Self Propelled Particles (SPPs). Granular rods with asymmetric mass distribution confined to move in a circular channel on a vibrated substrate and interact with each other through inelastic collision serve as our model vehicle. Motion of a single vehicle is observed to be composed of 2 parts, a linear velocity in the direction of lighter end of particle and a non-Gaussian random velocity. We find that the collective mean speed of the SPPs is constant over a wide range of line densities before decreasing rapidly as the maximum packing is approached indicating the spontaneous formation of Phantom jams. This decrease in speed is observed to be far greater than any small differences in the mean drift speed of individual SPPs , and occurs as the collision frequency between SPPs increase exponentially with line density. However the random velocity component of SPPs remain super-diffusive over entire range of line densities. While the collective motion at low densities is characterized by caravan following behind the slowest particle leading to clustering, at higher densities we see formation of jamming waves travelling in direction opposite to that of motion of particles.

  4. Role of Intermediate Filaments in Vesicular Traffic

    Directory of Open Access Journals (Sweden)

    Azzurra Margiotta

    2016-04-01

    Full Text Available Intermediate filaments are an important component of the cellular cytoskeleton. The first established role attributed to intermediate filaments was the mechanical support to cells. However, it is now clear that intermediate filaments have many different roles affecting a variety of other biological functions, such as the organization of microtubules and microfilaments, the regulation of nuclear structure and activity, the control of cell cycle and the regulation of signal transduction pathways. Furthermore, a number of intermediate filament proteins have been involved in the acquisition of tumorigenic properties. Over the last years, a strong involvement of intermediate filament proteins in the regulation of several aspects of intracellular trafficking has strongly emerged. Here, we review the functions of intermediate filaments proteins focusing mainly on the recent knowledge gained from the discovery that intermediate filaments associate with key proteins of the vesicular membrane transport machinery. In particular, we analyze the current understanding of the contribution of intermediate filaments to the endocytic pathway.

  5. EMPIRICAL VERIFICATION OF ANISOTROPIC HYDRODYNAMIC TRAFFIC MODEL IN TRAFFIC ANALYSIS AT INTERSECTIONS IN URBAN AREA

    Institute of Scientific and Technical Information of China (English)

    WEI Yan-fang; GUO Si-ling; XUE Yu

    2007-01-01

    In this article, the traffic hydrodynamic model considering the driver's reaction time was applied to the traffic analysis at the intersections on real roads. In the numerical simulation with the model, the pinch effect of the right-turning vehicles flow was found, which mainly leads to traffic jamming on the straight lane. All of the results in accordance with the empirical data confirm the applicability of this model.

  6. Assessment of network traffic congestion through Traffic Congestability Value (TCV): a new index

    OpenAIRE

    Patel Nilanchal; Mukherjee Alok Bhushan

    2015-01-01

    Traffic congestion is a major and growing problem in urban areas across the globe. It reduces the effective spatial interaction between different locations. To mitigate traffic congestion, not only the actual status of different routes needs to be known but also it is imperative to determine network congestion in different spatial zones associated with distinct land use classes. In the present paper, a new formula is proposed to quantify traffic congestion in the different spatial zones of a ...

  7. 32 CFR 634.26 - Traffic law enforcement principles.

    Science.gov (United States)

    2010-07-01

    ... applies proper enforcement measures to traffic congestion and focuses on selected time periods, conditions... 32 National Defense 4 2010-07-01 2010-07-01 true Traffic law enforcement principles. 634.26... ENFORCEMENT AND CRIMINAL INVESTIGATIONS MOTOR VEHICLE TRAFFIC SUPERVISION Traffic Supervision § 634.26...

  8. Traffic Driven Analysis of Cellular and WiFi Networks

    Science.gov (United States)

    Paul, Utpal Kumar

    2012-01-01

    Since the days Internet traffic proliferated, measurement, monitoring and analysis of network traffic have been critical to not only the basic understanding of large networks, but also to seek improvements in resource management, traffic engineering and security. At the current times traffic in wireless local and wide area networks are facing…

  9. ON THE RELEVANCE OF ON-LINE TRAFFIC ENGINEERING

    NARCIS (Netherlands)

    Fu, B.; Uhlig, S.P.W.G.

    The evaluation of dynamic Traffic Engineering (TE) algorithms is usually carried out using some specific network(s), traffic pattern(s) and traffic engineering objective(s). As the behavior of a TE algorithm is a consequence of the interactions between the network, the traffic demand and the algorit

  10. Linking network usage patterns to traffic Gaussianity fit

    NARCIS (Netherlands)

    Oliveira Schmidt, de Ricardo; Sadre, Ramin; Melnikov, Nikolay; Schönwälder, Jürgen; Pras, Aiko

    2014-01-01

    Gaussian traffic models are widely used in the domain of network traffic modeling. The central assumption is that traffic aggregates are Gaussian distributed. Due to its importance, the Gaussian character of network traffic has been extensively assessed by researchers in the past years. In 2001, res

  11. 36 CFR 4.12 - Traffic control devices.

    Science.gov (United States)

    2010-07-01

    ... 36 Parks, Forests, and Public Property 1 2010-07-01 2010-07-01 false Traffic control devices. 4.12... VEHICLES AND TRAFFIC SAFETY § 4.12 Traffic control devices. Failure to comply with the directions of a traffic control device is prohibited unless otherwise directed by the superintendent....

  12. 36 CFR 1004.12 - Traffic control devices.

    Science.gov (United States)

    2010-07-01

    ... 36 Parks, Forests, and Public Property 3 2010-07-01 2010-07-01 false Traffic control devices. 1004.12 Section 1004.12 Parks, Forests, and Public Property PRESIDIO TRUST VEHICLES AND TRAFFIC SAFETY § 1004.12 Traffic control devices. Failure to comply with the directions of a traffic control device...

  13. Economic Analysis of Traffic Flow with an Evolution Equation

    Institute of Scientific and Technical Information of China (English)

    冯苏苇

    2005-01-01

    Based on two main hypotheses of traffic economical equilibrium and the relationship between traffic density and the demand, an evolution equation of traffic cost was proposed to describe the change of cost under decreasing toll. Economical explanation of the model and a numerical case were given to demonstrate the constraint between the marginal traffic demand and the flow velocity.

  14. Recovery from blocking between outcomes.

    Science.gov (United States)

    Wheeler, Daniel S; Miller, Ralph R

    2005-10-01

    Contemporary associative learning research largely focuses on cue competition phenomena that occur when 2 cues are paired with a common outcome. Little research has been conducted to investigate similar phenomena occurring when a single cue is trained with 2 outcomes. Three conditioned lick suppression experiments with rats assessed whether treatments known to alleviate blocking between cues would also attenuate blocking between outcomes. In Experiment 1, conditioned responding recovered from blocking between outcomes when a long retention interval was interposed between training and testing. Experiment 2 obtained recovery from blocking between outcomes when the blocking outcome was extinguished after the blocking treatment. In Experiment 3, a recovery from blocking between outcomes occurred when a reminder stimulus was presented in a novel context prior to testing. Collectively, these studies demonstrate that blocking of outcomes, like blocking of cues, appears to be caused by a deficit in the expression of an acquired association.

  15. Caltrans Average Annual Daily Traffic Volumes (2004)

    Data.gov (United States)

    California Environmental Health Tracking Program — [ from http://www.ehib.org/cma/topic.jsp?topic_key=79 ] Traffic exhaust pollutants include compounds such as carbon monoxide, nitrogen oxides, particulates (fine...

  16. A Robust Statistical Estimation of Internet Traffic

    CERN Document Server

    Chabchoub, Yousra; Guillemin, Fabrice; Robert, Philippe

    2009-01-01

    A new method of estimating flow characteristics in the Internet is developped in this paper. For this purpose, a new set of random variables (referred to as observables) is defined. When dealing with sampled traffic, these observables can easily be computed from sampled data. By adopting a convenient mouse/elephant dichotomy also {\\em dependent on traffic}, it is shown how these variables give a {\\em robust} statistical information of long flows. A mathematical framework is developed to estimate the accuracy of the method. As an application, it is shown how one can estimate the number of long TCP flows when only sampled traffic is available. The algorithm proposed is tested against experimental data collected from different types of IP traffic.

  17. A Brief Introduction of Air Traffic Management

    Institute of Scientific and Technical Information of China (English)

    1997-01-01

    Air Traffic Management(ATM)started publication in 1995 by Air Traffic Management Bureauof CAAC.It is the first magazine about ATM field in CAAC.The chairman of Editorial Board ofATM is Mr.Bao Peide,Vice Minister of CAAC.Mr.Chen Ziye,Vice President of the 1st ResearchInstitute of CAAC and Chen Xuhua,General Director of Air Traffic Management Bureau are vicechairmen of the editorial board,and six general directors of local air traffic management bureau andsome professors and experts are members of the editorial board.ATM includes more than 20 columns,which are communication,navigation,surveillance,air trafficmanagement,special articles(policy,planning and management of implementation of CNS/ATM

  18. Microscopic features of moving traffic jams

    CERN Document Server

    Kerner, B S; Klenov, S L; Rehborn, H; Hiller, Andreas; Kerner, Boris S.; Klenov, Sergey L.; Rehborn, Hubert

    2005-01-01

    Empirical and numerical microscopic features of moving traffic jams are presented. Based on a single vehicle data analysis, it is found that within wide moving jams, i.e., between the upstream and downstream jam fronts there is a complex microscopic spatiotemporal structure. This jam structure consists of alternations of regions in which traffic flow is interrupted and flow states of low speeds associated with "moving blanks" within the jam. Empirical features of the moving blanks are found. Based on microscopic models in the context of three-phase traffic theory, physical reasons for moving blanks emergence within wide moving jams are disclosed. Structure of moving jam fronts is studied based in microscopic traffic simulations. Non-linear effects associated with moving jam propagation are numerically investigated and compared with empirical results.

  19. Uninhabited Traffic Management System Evaluator (UTME) Project

    Data.gov (United States)

    National Aeronautics and Space Administration — The key innovation of this effort is the development of an Uninhabited Aerial System (UAS) Traffic Manager Evaluator (UTME) specifically targeted at evaluating...

  20. Air Traffic Management Cost Assessment Tool Project

    Data.gov (United States)

    National Aeronautics and Space Administration — The Robust Analytics air traffic management cost assessment tool provides the comprehensive capability to analyze the impacts of NASA Airspace Systems Program (ASP)...

  1. Network Characteristics of Video Streaming Traffic

    CERN Document Server

    Rao, Ashwin; Barakat, Chadi; Legout, Arnaud; Towsley, Don; Dabbous, Walid

    2011-01-01

    Video streaming represents a large fraction of Internet traffic. Surprisingly, little is known about the network characteristics of this traffic. In this paper, we study the network characteristics of the two most popular video streaming services, Netflix and YouTube. We show that the streaming strategies vary with the type of the application (Web browser or native mobile application), and the type of container (Silverlight, Flash, or HTML5) used for video streaming. In particular, we identify three different streaming strategies that produce traffic patterns from non-ack clocked ON-OFF cycles to bulk TCP transfer. We then present an analytical model to study the potential impact of these streaming strategies on the aggregate traffic and make recommendations accordingly.

  2. Railway Network Timetabling and Dynamic Traffic Management

    NARCIS (Netherlands)

    Hansen, I.A.

    2009-01-01

    The paper discusses the current state of research concerning railway network timetabling and traffic management. Timetable effectiveness is governed by frequency, regularity, accurate running, recovery and layover times, as well as minimal headway, buffer times and waiting times. Analytic (queuing)

  3. Traffic Flow Density Distribution Based on FEM

    Science.gov (United States)

    Ma, Jing; Cui, Jianming

    In analysis of normal traffic flow, it usually uses the static or dynamic model to numerical analyze based on fluid mechanics. However, in such handling process, the problem of massive modeling and data handling exist, and the accuracy is not high. Finite Element Method (FEM) is a production which is developed from the combination of a modern mathematics, mathematics and computer technology, and it has been widely applied in various domain such as engineering. Based on existing theory of traffic flow, ITS and the development of FEM, a simulation theory of the FEM that solves the problems existing in traffic flow is put forward. Based on this theory, using the existing Finite Element Analysis (FEA) software, the traffic flow is simulated analyzed with fluid mechanics and the dynamics. Massive data processing problem of manually modeling and numerical analysis is solved, and the authenticity of simulation is enhanced.

  4. Lead levels on traffic-less islands.

    Science.gov (United States)

    Elwood, P C; Blaney, R; Robb, R C; Essex-Cater, A J; Davies, B E; Toothill, C

    1985-09-01

    Surveys were conducted on three traffic-less islands: Tory and Aran, off the coast of Ireland, and Sark, one of the Channel Islands off the coast of France. Identical methods were used in surveys in three other areas, all of which have heavy gasoline driven traffic. These were Jersey, another of the Channel Islands, Ebbw Vale--a mixed industrial area, and Cardiff--the capital city of Wales. Environmental lead levels were very low in two of the traffic-less islands, but on the third, house dust lead levels were comparable with levels found throughout Wales. Blood lead levels on one of the islands were similar to those which have been reported for unaccultured remote tribes, but on the other two traffic-less islands blood lead levels were comparable with those of areas on the mainland of Wales.

  5. Review of traffic conflicts technique studies.

    NARCIS (Netherlands)

    Hondel, M. van den & Kraay, J.H.

    1979-01-01

    A review of literature concerning traffic conflicts technique studies is presented. The 101 references arranged alphabetically by authors' names are given with abstract. The procedure used in compiling this survey is outlined.

  6. SUPERFICIAL CERVICAL PLEXUS BLOCK

    Directory of Open Access Journals (Sweden)

    Komang Mega Puspadisari

    2014-01-01

    Full Text Available Superficial cervical plexus block is one of the regional anesthesia in  neck were limited to thesuperficial fascia. Anesthesia is used to relieve pain caused either during or after the surgery iscompleted. This technique can be done by landmark or with ultrasound guiding. The midpointof posterior border of the Sternocleidomastoid was identified and the prosedure done on thatplace or on the level of cartilage cricoid.

  7. Research on traffic congestion mechanism and countermeasures based on dynamic traffic assignment

    Institute of Scientific and Technical Information of China (English)

    PEI Yu-long; LANG Yi-shun

    2005-01-01

    Traffic congestion is widely distributed around a network. Generally, to analyze traffic congestion, static traffic capacity is adopted. But dynamic characteristics must be studied because congestion is a dynamic process. A Dynamic Traffic Assignment modeling fundamental combined with an urban congestion analysis method is studied in this paper. Three methods are based on congestion analysis, and the stochastic user optimal DTA models are especially considered. Correspondingly, a dynamic system optimal model is suggested for responding congestion countermeasures and an ideal user optimal model for predicted congestion countermeasure respectively.

  8. Managing access block.

    Science.gov (United States)

    Cameron, Peter; Scown, Paul; Campbell, Donald

    2002-01-01

    There is pessimism regarding the ability of the Acute Health Sector to manage access block for emergency and elective patients. Melbourne Health suffered an acute bed crisis in 2001 resulting in record ambulance diversions and emergency department (ED) delays. We conducted an observational study to reduce access block for emergency patients whilst maintaining elective throughput at Melbourne Health. This involved a clinician-led taskforce using previously proven principles for organisational change to implement 51 actions to improve patient access over a three-month period. The primary outcome measures were ambulance diversion, emergency patients waiting more than 12 hours for an inpatient bed, elective throughput and theatre cancellations. Despite a reduction in multi-day bed numbers all primary objectives were met, ambulance diversion decreased to minimal levels, 12-hour waits decreased by 40% and elective throughput was maintained. Theatre cancellations were also minimised. We conclude that access block can be improved by clinician-led implementation of proven process improvements over a short time frame. The ability to sustain change over the longer term requires further study.

  9. Wavelet Neural Network Based Traffic Prediction for Next Generation Network

    Institute of Scientific and Technical Information of China (English)

    Zhao Qigang; Li Qunzhan; He Zhengyou

    2005-01-01

    By using netflow traffic collecting technology, some traffic data for analysis are collected from a next generation network (NGN) operator. To build a wavelet basis neural network (NN), the Sigmoid function is replaced with the wavelet in NN. Then the wavelet multiresolution analysis method is used to decompose the traffic signal, and the decomposed component sequences are employed to train the NN. By using the methods, an NGN traffic prediction model is built to predict one day's traffic. The experimental results show that the traffic prediction method of wavelet NN is more accurate than that without using wavelet in the NGN traffic forecasting.

  10. Traffic Monitoring and Control Using RFID

    Directory of Open Access Journals (Sweden)

    Gaurav Thakur

    2013-09-01

    Full Text Available Traffic congestion and tidal flow management were recognized as major problems in modern urban areas, which have caused much frustration and loss of man hours. The operation of standard traffic lights which are currently deployed in many junctions, are based on predetermined timing schemes, which are fixed during the installation and remain until further resetting. The timing is no more than a default setup to control what may be considered as normal traffic. Although every road junction by necessity requires different traffic light timing setup, many existing systems operate with a default sequence. To design an intelligent and efficient traffic control system, a number of parameters that represent the status of the road conditions must be identified and taken into consideration. Approach: In order to solve the problem an intelligent RFID traffic control has been developed. This project is based on monitoring the traffic density at the signal using RFID Reader. RFID tags which is placed (instead of number plate at each vehicle will serve as the unique number of vehicle .Reader will count the number of tags at the signal and take decision of increasing or decreasing the signal time .This reader is also use to find the violation(VIN of violator of the signal. Each passive tag will have certain number of bytes information will be different for different vehicles. Whenever any tag will approach any signal, reader will take that bytes of information and simultaneously will also increment the number of vehicles for making decision to change the signal timing. Each tag of data will be stored in main memory (database.This unique data will also help in determining the violator of the signal .Thus it will save human efforts and resources to a greater extent and reduce waiting of the people at the signal. After implementation of this project, now all people waiting at the signal will get proper time (green light according to the traffic density

  11. Injuries to pedestrians in road traffic accidents.

    OpenAIRE

    Atkins, R. M.; Turner, W H; Duthie, R. B.; Wilde, B. R.

    1988-01-01

    Although there have been many reports on injuries to occupants of cars in road traffic accidents, there have been few prospective studies of injuries to pedestrians in such accidents. For this reason a two year prospective study of pedestrians in road traffic accidents in the Oxford region was carried out. The incidence of death in pedestrians was significantly higher than in car occupants or motorcyclists. The principal determinant of death was the weight of the vehicle concerned. The most c...

  12. Bayesian Particle Tracking of Traffic Flows

    OpenAIRE

    2014-01-01

    We develop a Bayesian particle filter for tracking traffic flows that is capable of capturing non-linearities and discontinuities present in flow dynamics. Our model includes a hidden state variable that captures sudden regime shifts between traffic free flow, breakdown and recovery. We develop an efficient particle learning algorithm for real time on-line inference of states and parameters. This requires a two step approach, first, resampling the current particles, with a mixture predictive ...

  13. Effects of Bottlenecks in Vehicle Traffic

    CERN Document Server

    Yamamoto, S; Tadaki, S; Yamamoto, Syohei; Hieida, Yasuhiro; Tadaki, Shin-ichi

    2006-01-01

    Traffic congestion is usually observed at the upper streams of bottlenecks like tunnels. Congestion appears as stop-and-go waves and high density uniform flow. We perform simulations of traffic flow with a bottleneck using the coupled map optimal velocity model. The bottleneck is expressed as a road segment with speed reduction. The emergence of stop-and-go waves depends on the speed reduction in the bottleneck. A phenomenological theory of bottleneck effects is constructed.

  14. Modelling of urban traffic networkof signalized intersections

    OpenAIRE

    2013-01-01

    This report presents how traffic network of signalized intersection in a chosen urban area called Tema is synchronized. Using a modular approach, two different types of traffic intersection commonly found in an urban area were modelled i.e. a simple intersection and a complex intersection. A direct road, even though not an intersection, was also included in the modelling because it’s commonly found in an urban area plus it connects any two intersections. Each of these scenarios was modelled u...

  15. Traffic Police in Ji’nan

    Institute of Scientific and Technical Information of China (English)

    1997-01-01

    The 13 traffic policewomen of No. 7 Ergang Road in the city of Ji’nan. Shandong Province, hold a special feminine spiritual outlook. Wearing their olive green berets, under the guidance of Captain Shi Li, they began to take point duty at the traffic stand on October 1, 1996. Graduates of the Shandong Public Security Professional College at the average age of 22, these women began formal group training in Sep

  16. Intelligent traffic control system using PLC

    Science.gov (United States)

    Barz, C.; Todea, C.; Latinovic, T.; Preradovic, D. M.; Deaconu, S.; Berdie, A.

    2016-08-01

    The paper presents the traffic control system controlled through a PLC which takes the signals from different sensors on roads. The global system developed ensures the coordination of four intersections, setting a path that respects coordination type green light, the integration of additional sensors, the implementation of probes radar to inform traffic participants about recommended speed for accessing the green state located in the intersection that will follow to cross.

  17. Visual Analysis of Air Traffic Data

    Science.gov (United States)

    Albrecht, George Hans; Pang, Alex

    2012-01-01

    In this paper, we present visual analysis tools to help study the impact of policy changes on air traffic congestion. The tools support visualization of time-varying air traffic density over an area of interest using different time granularity. We use this visual analysis platform to investigate how changing the aircraft separation volume can reduce congestion while maintaining key safety requirements. The same platform can also be used as a decision aid for processing requests for unmanned aerial vehicle operations.

  18. Online traffic flow model applying dynamic flow-density relation

    CERN Document Server

    Kim, Y

    2002-01-01

    This dissertation describes a new approach of the online traffic flow modelling based on the hydrodynamic traffic flow model and an online process to adapt the flow-density relation dynamically. The new modelling approach was tested based on the real traffic situations in various homogeneous motorway sections and a motorway section with ramps and gave encouraging simulation results. This work is composed of two parts: first the analysis of traffic flow characteristics and second the development of a new online traffic flow model applying these characteristics. For homogeneous motorway sections traffic flow is classified into six different traffic states with different characteristics. Delimitation criteria were developed to separate these states. The hysteresis phenomena were analysed during the transitions between these traffic states. The traffic states and the transitions are represented on a states diagram with the flow axis and the density axis. For motorway sections with ramps the complicated traffic fl...

  19. Competitive Traffic Assignment in Road Networks

    Directory of Open Access Journals (Sweden)

    Krylatov Alexander Y.

    2016-09-01

    Full Text Available Recently in-vehicle route guidance and information systems are rapidly developing. Such systems are expected to reduce congestion in an urban traffic area. This social benefit is believed to be reached by imposing the route choices on the network users that lead to the system optimum traffic assignment. However, guidance service could be offered by different competitive business companies. Then route choices of different mutually independent groups of users may reject traffic assignment from the system optimum state. In this paper, a game theoretic approach is shown to be very efficient to formalize competitive traffic assignment problem with various groups of users in the form of non-cooperative network game with the Nash equilibrium search. The relationships between the Wardrop’s system optimum associated with the traffic assignment problem and the Nash equilibrium associated with the competitive traffic assignment problem are investigated. Moreover, some related aspects of the Nash equilibrium and the Wardrop’s user equilibrium assignments are also discussed.

  20. Congestion and communication in confined ant traffic

    Science.gov (United States)

    Gravish, Nick; Gold, Gregory; Zangwill, Andrew; Goodisman, Michael A. D.; Goldman, Daniel I.

    2014-03-01

    Many social animals move and communicate within confined spaces. In subterranean fire ants Solenopsis invicta, mobility within crowded nest tunnels is important for resource and information transport. Within confined tunnels, communication and traffic flow are at odds: trafficking ants communicate through tactile interactions while stopped, yet ants that stop to communicate impose physical obstacles on the traffic. We monitor the bi-directional flow of fire ant workers in laboratory tunnels of varied diameter D. The persistence time of communicating ant aggregations, τ, increases approximately linearly with the number of participating ants, n. The sensitivity of traffic flow increases as D decreases and diverges at a minimum diameter, Dc. A cellular automata model incorporating minimal traffic features--excluded volume and communication duration--reproduces features of the experiment. From the model we identify a competition between information transfer and the need to maintain jam-free traffic flow. We show that by balancing information transfer and traffic flow demands, an optimum group strategy exists which maximizes information throughput. We acknowledge funding from NSF PoLS #0957659 and #PHY-1205878.

  1. Urban planning and traffic safety at night

    Science.gov (United States)

    Ispas, N.; Trusca, D.

    2016-08-01

    Urban planning including traffic signs serve vital functions, providing road users with regulatory, warning and guidance information about the roadway and surrounding environment. There are a large number of signs and even more guidelines on how these signs should be designed, installed, and maintained in concordance with on road surface traffic signs. More requirements for signs are made for night urban traffic, including appearance (size, shape, colour), placement (height, lateral, and longitudinal), maintenance (visibility, position, damage) and signs light and retroreflective. In the night, traffic signs visibility can interact by on pedestrian visibility and diminish urban traffic safety. The main aim of this paper are the scientific determination of an urban specific zone visibility for evaluate at night real conditions in case of a traffic accident in the Braşov city area. The night visibility study was made using PC-Rect version 4.2. Other goal of the paper was to modify some urban planning solution in order to increase the urban safety in Brașov.

  2. Optimizing Traffic Operation in Designing Specific Upgrades

    Directory of Open Access Journals (Sweden)

    Ebrahim Sangsefidi

    2015-01-01

    Full Text Available Transport forms one of the primary needs in all categories of the population in modern society; it is of paramount concern for traffic engineers, transport planners, and policy makers to understand and evaluate the quality of service being provided by the transport facilities designed by them. This paper presents an investigation in profile geometric design and traffic flow operation on two-lane two-way highways and provides analyses that will help in a better understanding of traffic operation on these facilities to select the optimum profile configuration. The effects of influencing parameters consisting of grade, length of grade, traffic composition, and traffic volume are evaluated and finally a systematic procedure to evaluate flow rate under the base condition is presented. Finally, based on these achievements an algorithm is introduced to select optimum Finished Ground of profile view. Results show that the percentage of heavy vehicles has a contributing effect on traffic operation so that the optimum profile configuration is incredibly affected by this factor. Source data have been obtained from Highway Capacity Manual (HCM as a pioneer document in respect of quantifying the concept of capacity for a transport facility.

  3. System and method for traffic signal timing estimation

    KAUST Repository

    Dumazert, Julien

    2015-12-30

    A method and system for estimating traffic signals. The method and system can include constructing trajectories of probe vehicles from GPS data emitted by the probe vehicles, estimating traffic signal cycles, combining the estimates, and computing the traffic signal timing by maximizing a scoring function based on the estimates. Estimating traffic signal cycles can be based on transition times of the probe vehicles starting after a traffic signal turns green.

  4. Block Transfer Handbook: Constructing and Negotiating Block Transfer Agreements.

    Science.gov (United States)

    Finlay, Finola

    The purpose of this handbook is to provide resources for institutions or articulation committees who are engaged in the task of investigating the feasibility of block transfer agreements. Block transfer is the process whereby a block of credits is granted to students who have successfully completed a certificate, diploma, or cluster of courses…

  5. Impacts of Traffic Noise and Traffic Volume on Birds of Roadside Habitats

    Directory of Open Access Journals (Sweden)

    Kirsten M. Parris

    2009-06-01

    Full Text Available Roadside habitats are important for a range of taxa including plants, insects, mammals, and birds, particularly in developed countries in which large expanses of native vegetation have been cleared for agriculture or urban development. Although roadside vegetation may provide suitable habitat for many species, resident animals can be exposed to high levels of traffic noise, visual disturbance from passing vehicles, and the risk of collision with cars and trucks. Traffic noise can reduce the distance over which acoustic signals such as song can be detected, an effect known as acoustic interference or masking. Studies from the northern hemisphere show that the singing behavior of birds changes in the presence of traffic noise. We investigated the impact of traffic noise and traffic volume on two species of birds, the Grey Shrike-thrush (Colluricincla harmonica and the Grey Fantail (Rhipidura fuliginosa, at 58 roadside sites on the Mornington Peninsula, southeastern Australia. The lower singing Grey Shrike-thrush sang at a higher frequency in the presence of traffic noise, with a predicted increase in dominant frequency of 5.8 Hz/dB of traffic noise, and a total effect size of 209 Hz. In contrast, the higher singing Grey Fantail did not appear to change its song in traffic noise. The probability of detecting each species on a visit to a site declined substantially with increasing traffic noise and traffic volume, with several lines of evidence supporting a larger effect of traffic noise. Traffic noise could hamper detection of song by conspecifics, making it more difficult for birds to establish and maintain territories, attract mates and maintain pair bonds, and possibly leading to reduced breeding success in noisy roadside habitats. Closing key roads during the breeding season is a potential, but untested, management strategy to protect threatened bird species from traffic noise and collision with vehicles at the time of year when they are most

  6. Network Analysis of Urban Traffic with Big Bus Data

    CERN Document Server

    Zhao, Kai

    2016-01-01

    Urban traffic analysis is crucial for traffic forecasting systems, urban planning and, more recently, various mobile and network applications. In this paper, we analyse urban traffic with network and statistical methods. Our analysis is based on one big bus dataset containing 45 million bus arrival samples in Helsinki. We mainly address following questions: 1. How can we identify the areas that cause most of the traffic in the city? 2. Why there is a urban traffic? Is bus traffic a key cause of the urban traffic? 3. How can we improve the urban traffic systems? To answer these questions, first, the betweenness is used to identify the most import areas that cause most traffics. Second, we find that bus traffic is not an important cause of urban traffic using statistical methods. We differentiate the urban traffic and the bus traffic in a city. We use bus delay as an identification of the urban traffic, and the number of bus as an identification of the bus traffic. Third, we give our solutions on how to improve...

  7. Apm4, the mu subunit of yeast AP-2 interacts with Pkc1, and mutation of the Pkc1 consensus phosphorylation site Thr176 inhibits AP-2 recruitment to endocytic sites

    Science.gov (United States)

    Chapa-y-Lazo, Bernardo; Ayscough, Kathryn R

    2014-01-01

    The AP-2 endocytic adaptor has been extensively characterized in mammalian cells and is considered to play a role both in cargo binding and in formation of endocytic sites. However, despite our detailed knowledge of mechanistic aspects of endocytic complex assembly and disassembly in the model organism Saccharomyces cerevisiae, no function of AP-2 had been described in wild-type yeast under normal growth conditions. A recent study however revealed that disruption of the complex caused by deletion of the gene encoding its mu subunit (APM4) caused defects in cell polarity such that responses to pheromone, nutritional status and cell wall damage were affected. Furthermore, a homozygous deletion of the mu subunit gene in Candida albicans affected its ability to grow hyphae. Direct binding to the yeast cell wall stress sensor Mid2 was detected, and in an apm4 deletion strain Mid2 showed reduced re-localization to the mother bud neck region following cell wall damage with calcofluor or to the mating projection tip. Here we demonstrate an interaction between Apm4 and the yeast cell wall integrity pathway component Pkc1 and show that mutation of the predicted Pkc1 site in the Apm4 hinge region affects recruitment of the AP-2 complex to endocytic sites. PMID:25346786

  8. Demographic Data - MDC_Block

    Data.gov (United States)

    NSGIC GIS Inventory (aka Ramona) — A polygon feature class of Miami-Dade Census 2000 Blocks. Census blocks are areas bounded on all sides by visible and/or invisible features shown on a map prepared...

  9. Habitat Blocks and Wildlife Corridors

    Data.gov (United States)

    Vermont Center for Geographic Information — Habitat blocks are areas of contiguous forest and other natural habitats that are unfragmented by roads, development, or agriculture. Vermonts habitat blocks are...

  10. Porous block nanofiber composite filters

    Energy Technology Data Exchange (ETDEWEB)

    Ginley, David S.; Curtis, Calvin J.; Miedaner, Alexander; Weiss, Alan J.; Paddock, Arnold

    2016-08-09

    Porous block nano-fiber composite (110), a filtration system (10) and methods of using the same are disclosed. An exemplary porous block nano-fiber composite (110) includes a porous block (100) having one or more pores (200). The porous block nano-fiber composite (110) also includes a plurality of inorganic nano-fibers (211) formed within at least one of the pores (200).

  11. Properties of blocked linear systems.

    Science.gov (United States)

    Chen, Weitian; Anderson, Brian D O; Deistler, Manfred; Filler, Alexander

    2012-10-01

    This paper presents a systematic study on the properties of blocked linear systems that have resulted from blocking discrete-time linear time invariant systems. The main idea is to explore the relationship between the blocked and the unblocked systems. Existing results are reviewed and a number of important new results are derived. Focus is given particularly on the zero properties of the blocked system as no such study has been found in the literature.

  12. Appropriate schemata and building blocks

    Institute of Scientific and Technical Information of China (English)

    Yang Haijun; Li Minqiang

    2005-01-01

    Appropriate schemata as a novel concept to characterize building blocks are introduced, and then, the traits of appropriate schemata are presented. The effects of building blocks by search operators are analyzed. Hence, the experiments on RR-8X8 are employed to verify that appropriate schemata construct the building blocks. The validity of appropriate schemata and building blocks from the views of theory and practice is presented.

  13. Traffic Analysis Zones - TRAFFIC_ZONES_TIGER00_IN: Indiana Traffic Analysis Zones (U.S. Census Bureau, 1:100,000, Polygon Shapefile)

    Data.gov (United States)

    NSGIC GIS Inventory (aka Ramona) — TRAFFIC_ZONES_TIGER00_IN contains traffic analysis zones in Indiana identified by the US Bureau of the Census. Vigo, Madison, and Monroe counties were not available...

  14. Blocking the Hawking radiation

    DEFF Research Database (Denmark)

    Autzen, M.; Kouvaris, C.

    2014-01-01

    grows after its formation (and eventually destroys the star) instead of evaporating. The fate of the black hole is dictated by the two opposite mechanics, i.e., accretion of nuclear matter from the center of the star and Hawking radiation that tends to decrease the mass of the black hole. We study how...... the assumptions for the accretion rate can in fact affect the critical mass beyond which a black hole always grows. We also study to what extent degenerate nuclear matter can impede Hawking radiation due to the fact that emitted particles can be Pauli blocked at the core of the star....

  15. Adaptive Delayed Acknowledgement Algorithm for MPEG-4 Traffic in UWB Networks

    Institute of Scientific and Technical Information of China (English)

    LIU Xin; DAI Qionghai; WU Qiufeng

    2006-01-01

    Although ultra-wideband transmission rates are very high in the physical layer, the bandwidth utili-zation efficiency is very low in the medium access control (MAC) layer. The bandwidth utilization efficiencycan be improved and the quality of service for delay-sensitive variable bit rate traffic such as high rate mo-tion picture experts group (MPEG)-4 traffic can be guaranteed by a simple and efficient algorithm that dy-namically sets the burst size of the delayed-acknowledgement (Dly-ACK) based on the IEEE 802.15.3 stan-dard. The burst size was adjusted in response to the wireless channel quality. Furthermore, the burst sizewas bounded by a maximum acknowledgement burst size setting and the remaining number of un-receivedblocks of a current supra-block. Simulation results show that the method achieves significant performanceenhancement in the job failure rate and throughput compared to immediate-acknowledgement (Imm-ACK)and other fixed burst size Dly-ACK schemes. This algorithm can also be applied in generalized time divisionmultiple access (TDMA) systems by using the block acknowledgement mechanism for delay sensitive traffic.

  16. COST 329 : towards European models for traffic and traffic safety developments.

    NARCIS (Netherlands)

    Oppe, S.

    1996-01-01

    The aim of COST Activity 329 is to specify an efficient methodology for the analysis of past and future traffic safety developments based on available knowledge. This includes the applicability of time-series modelling techniques. It is planned to make use of traffic speed developments to detect une

  17. Tri-level programming model for combined urban traffic signal control and traffic flow guidance

    Institute of Scientific and Technical Information of China (English)

    孙智源; 陆化普; 屈闻聪

    2016-01-01

    In order to balance the temporal-spatial distribution of urban traffic flow, a model is established for combined urban traffic signal control and traffic flow guidance. With consideration of the wide use of fixed signal control at intersections, traffic assignment under traffic flow guidance, and dynamic characteristics of urban traffic management, a tri-level programming model is presented. To reflect the impact of intersection delay on traffic assignment, the lower level model is set as a modified user equilibrium model. The middle level model, which contains several definitional constraints for different phase modes, is built for the traffic signal control optimization. To solve the problem of tide lane management, the upper level model is built up based on nonlinear 0-1 integer programming. A heuristic iterative optimization algorithm (HIOA) is set up to solve the tri-level programming model. The lower level model is solved by method of successive averages (MSA), the middle level model is solved by non-dominated sorting genetic algorithm II (NSGA II), and the upper level model is solved by genetic algorithm (GA). A case study is raised to show the efficiency and applicability of the proposed modelling and computing method.

  18. Property Blocks: Games and Activities.

    Science.gov (United States)

    Humphreys, Alan, Ed.; Dailey, Jean, Ed.

    This pamphlet describes the property blocks produced by MINNEMAST, and discusses their use in the development of thinking processes. Classification systems, including block diagrams and tree diagrams, are discussed. Sixteen classroom activities and eleven games which use the blocks are described. Suggestions to the teacher for further reading are…

  19. Eps15 is recruited to the plasma membrane upon epidermal growth factor receptor activation and localizes to components of the endocytic pathway during receptor internalization

    DEFF Research Database (Denmark)

    Torrisi, M R; Lotti, L V; Belleudi, F;

    1999-01-01

    Eps15 is a substrate for the tyrosine kinase of the epidermal growth factor receptor (EGFR) and is characterized by the presence of a novel protein:protein interaction domain, the EH domain. Eps15 also stably binds the clathrin adaptor protein complex AP-2. Previous work demonstrated an essential...... role for eps15 in receptor-mediated endocytosis. In this study we show that, upon activation of the EGFR kinase, eps15 undergoes dramatic relocalization consisting of 1) initial relocalization to the plasma membrane and 2) subsequent colocalization with the EGFR in various intracellular compartments...... of the endocytic pathway, with the notable exclusion of coated vesicles. Relocalization of eps15 is independent of its binding to the EGFR or of binding of the receptor to AP-2. Furthermore, eps15 appears to undergo tyrosine phosphorylation both at the plasma membrane and in a nocodazole-sensitive compartment...

  20. Multicore-Optimized Wavefront Diamond Blocking for Optimizing Stencil Updates

    KAUST Repository

    Malas, T.

    2015-07-02

    The importance of stencil-based algorithms in computational science has focused attention on optimized parallel implementations for multilevel cache-based processors. Temporal blocking schemes leverage the large bandwidth and low latency of caches to accelerate stencil updates and approach theoretical peak performance. A key ingredient is the reduction of data traffic across slow data paths, especially the main memory interface. In this work we combine the ideas of multicore wavefront temporal blocking and diamond tiling to arrive at stencil update schemes that show large reductions in memory pressure compared to existing approaches. The resulting schemes show performance advantages in bandwidth-starved situations, which are exacerbated by the high bytes per lattice update case of variable coefficients. Our thread groups concept provides a controllable trade-off between concurrency and memory usage, shifting the pressure between the memory interface and the CPU. We present performance results on a contemporary Intel processor.