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Sample records for blister

  1. Fracture Blisters

    Directory of Open Access Journals (Sweden)

    Uebbing, Claire M

    2011-02-01

    Full Text Available Fracture blisters are a relatively uncommon complication of fractures in locations of the body, such as the ankle, wrist elbow and foot, where skin adheres tightly to bone with little subcutaneous fat cushioning. The blister that results resembles that of a second degree burn.These blisters significantly alter treatment, making it difficult to splint or cast and often overlying ideal surgical incision sites. Review of the literature reveals no consensus on management; however, most authors agree on early treatment prior to blister formation or delay until blister resolution before attempting surgical correction or stabilization. [West J Emerg Med. 2011;12(1;131-133.

  2. Blisters, First Aid

    Science.gov (United States)

    ... and rashes clinical tools newsletter | contact Share | Blisters, First Aid A A A Blisters on the feet are ... can also be found via the Disease List. First Aid Guide Blisters often go away on their own ...

  3. Decontamination Data - Blister Agents

    Data.gov (United States)

    U.S. Environmental Protection Agency — Decontamination efficacy data for blister agents on various building materials using various decontamination solutions This dataset is associated with the following...

  4. Critical metal blistering doses

    Energy Technology Data Exchange (ETDEWEB)

    Kalin, B.A.; Kirilin, N.M.; Pisarev, A.A.

    1975-08-01

    Critical He ion bombardment doses associated with blistering of Nb and stainless steel were measured. It was found that the critical doses for these materials are close together and in the range 1 to 4 x 10/sup 17/ ion/cm/sup 2/. (JRD)

  5. Skin friction blistering: computer model.

    OpenAIRE

    Xing, Malcolm; Pan, Ning; Zhong, Wen; Maibach, Howard

    2007-01-01

    BACKGROUND/PURPOSE: Friction blisters, a common injury in sports and military operations, can adversely effect or even halt performance. Given its frequency and hazardous nature, recent research efforts appear limited. Blistering can be treated as a delamination phenomenon; similar issues in materials science have been extensively investigated in theory and experiment. An obstacle in studying blistering is the difficulty of conducting experiment on humans and animals. Computer modeling thus b...

  6. Blistering of viscoelastic filaments

    CERN Document Server

    Sattler, R; Wagner, C

    2007-01-01

    When a dilute polymer solution experiences capillary thinning, it forms an almost uniformly cylindrical thread, which we study experimentally. In the last stages of thinning, when polymers have become fully stretched, the filament becomes prone to instabilities, of which we describe two: A novel "breathing" instability, originating from the edge of the filament, and a sinusoidal instability in the interior, which ultimately gives rise to a "blistering" pattern of beads on the filament. We describe the linear instability with a spatial resolution of 80 nm in the disturbance amplitude. For sufficiently high polymer concentrations, the filament eventually separates out into a "solid" phase of entangled polymers, connected by fluid beads. A solid polymer fiber of about 100 nanometer thickness remains, which is essentially permanent.

  7. AUTOIMMUNE EPIDERMAL BLISTERING DISEASES

    Directory of Open Access Journals (Sweden)

    Ana Maria Abreu Velez

    2013-11-01

    Full Text Available Autoimmune bullous skin diseases (ABDs are uncommon, potentially fatal diseases of skin and mucous membranes which are associated with deposits of autoantibodies and complement against distinct molecules of the epidermis and dermal/epidermal basement membrane zone (BMZ. These autoantibodies lead to a loss in skin molecular integrity, which manifests clinically as formation of blisters or erosions. In pemphigus vulgaris, loss of adhesion occurs within the epidermis. The pioneering work of Ernst H. Beutner, Ph.D. and Robert E. Jordon, M.D. confirmed the autoimmune nature of these diseases. Walter F. Lever, M.D. contributed significantly to our understanding of the histopathologic features of these diseases. Walter Lever, M.D. and Ken Hashimoto, M.D. contributed electron microscopic studies of these diseases, especially in pemphigus vulgaris and bullous pemphigoid. In bullous pemphigoid (BP, linear IgA bullous dermatosis, epidermolysis bullosa acquisita (EBA and dermatitis herpetiformis (DH, loss of adhesion takes place within or underneath the BMZ. Classic EBA demonstrates extensive skin fragility; DH is commonly associated with gluten-sensitive enteropathy, and manifests clinically with pruritic papulovesicles on the extensor surfaces of the extremities and the lumbosacral area. The clinical spectrum of bullous pemphigoid includes tense blisters, urticarial plaques, and prurigo-like eczematous lesions. Pemphigoid gestationis mostly occurs during the last trimester of pregnancy, and mucous membrane pemphigoid primarily involves the oral mucosa and conjunctivae and leads to scarring. Linear IgA bullous dermatosis manifests with tense blisters in a „cluster of jewels”-like pattern in childhood (chronic bullous disease of childhood and is more clinically heterogeneous in adulthood. Many of the autoantigens in these disorders are known and have been well characterized. ABDs may be influenced by both genetic and exogenous factors. The diagnoses of

  8. Initiating, growing and cracking of hydrogen blisters

    Institute of Scientific and Technical Information of China (English)

    REN Xuechong; SHAN Guangbin; CHU Wuyang; SU Yanjing; GAO Kewei; QIAO Lijie; JIANG Bo; CHEN Gang; CUI Yinhui

    2005-01-01

    The growing process of a hydrogen blister in a wheel steel was observed in situ with an optical microscope, and the fracture surfaces formed from broken blisters on a wheel steel and bulk metallic glass were investigated. The initiating, growing, cracking and breaking of hydrogen blisters are as follows. Supersaturated vacancies can increase greatly during charging and gather together into a vacancy cluster (small cavity). Hydrogen atoms become hydrogen molecules in the vacancy cluster and hydrogen molecules can stabilize the vacancy cluster. The small cavity becomes the nucleus of hydrogen blister. The blister will grow with entering of vacancies and hydrogen atoms. With increasing hydrogen pressure, plastic deformation occurs first, the hydrogen blister near the surface extrudes, and then cracks initiate along the wall of the blister with further increasing hydrogen pressure. A cracked blister can grow further through propagating of cracks until it breaks.

  9. Fracture Blisters After Primary Total Knee Arthroplasty.

    Science.gov (United States)

    Halawi, Mohamad J

    2015-08-01

    Fracture blisters are tense vesicles that arise on markedly swollen skin overlying traumatized soft tissue. While this relatively uncommon complication has been well described in the trauma literature, this article reports for the first time a case of fracture blisters after primary total knee arthroplasty. The fracture blisters developed within 36 hours of surgery and were associated with profound swelling and erythema. There was no evidence of vascular injury, compartment syndrome, iatrogenic fracture, or deep venous thrombosis. The patient was treated with leg elevation, loosely applied nonadhesive dressings, and a short course of oral antibiotics after skin desquamation. Blood-filled blisters required longer time to reepithelialization than fluid-filled blisters. Knee stiffness developed because of pain and fear of participation with physical therapy, but the patient was able to resume intensive rehabilitation after resolution of the blisters. Patient factors, surgical factors, and review of the literature are discussed. PMID:26251947

  10. WHITE BLISTER SPECIES (Albuginaceae ON WEEDS

    Directory of Open Access Journals (Sweden)

    Karolina Vrandečić

    2011-06-01

    Full Text Available The obligate fungi inside the family Albuginaceae are widespread world wide and cause white rust or white blister disease. Mycopopulation of weeds has been researched within the project „The role of weeds in epidemiology of row-crop diseases“. The aim of this research was to identify white blister species occurring on weeds in Eastern Croatia. Weed plants with disease symptoms characteristic for white blister species have been collected since 2001 on location Slavonia and Baranja country. Determination of white blister species was based on morphological characters of pathogen and the host. Wilsoniana bliti was determined on Amaranthus retroflexus and Amaranthus hybridus leaves. Capsella bursa pastoris is a host for Albugo candida. Ambrosia artemisiifolia is a host for Pustula sp. and Cirsium arvense was found to be host for Pustula spinulosa. Wilsoniana portulaceae was determined on Portulaca oleracea.

  11. A progressive pruritic rash with blisters.

    Science.gov (United States)

    Overcash, Michael D; Ewald, Matt

    2016-05-01

    This article describes a patient with a progressive pruritic rash and fluid-filled blisters. A punch biopsy later confirmed the diagnosis of bullous pemphigoid, an inflammatory condition that most commonly occurs in older adults and is treated with corticosteroids. PMID:27124233

  12. The mechanism of nucleation of hydrogen blister in metals

    Institute of Scientific and Technical Information of China (English)

    REN XueChong; ZHOU QingJun; CHU WuYang; LI JinXu; SU YanJing; QIAO LiJie

    2007-01-01

    The nucleating, growing and cracking of hydrogen blister have been investigated experimentally and theoretically. The results show that atomic hydrogen induces superabundant vacancies in metals. The superabundant vacancies and hydrogen aggregate into a hydrogen-vacancy cluster (microcavity). The hydrogen atoms in the microcavity become hydrogen molecules which can stabilize the cluster. And the hydrogen blister nucleates. With the entry of vacancies and hydrogen atoms, the blister nucleus grows and the pressure in the cavity increases. When the stress induced by hydrogen pressure on the blister is up to the cohesive strength, cracks will initiate from the wall of the blister.

  13. Delayed postburn blisters: an immunohistochemical and ultrastructural study.

    Science.gov (United States)

    Bergman, R; David, R; Ramon, Y; Ramon, M; Kerner, H; Kilim, S; Peled, I; Friedman-Birnbaum, R

    1997-08-01

    This study was performed in an attempt to further elucidate the pathogenesis of delayed postburn blistering. Two cases were studied ultrastructurally and immunohistochemically, 1 with blisters on the recipient site of autologous split-thickness skin grafts and the other on the donor site. Ultrastructurally, the basement membrane was on the roof of the blisters in both cases, except for a single small blister in the first case where it was on the dermal floor. In the blister roofs, the basement membrane showed small or marked segments of discontinuity. In the adjacent non-blistered healed skin, the basement membrane was usually continuous, and anchoring fibrils were present. Immunoperoxidase staining on frozen sections, using antibodies to laminin, laminin 5, collagen IV, and collagen VII, showed a mostly continuous linear pattern in the adjacent non-blistered skin, which often became discontinuous near the blisters and markedly discontinuous in the blister roofs. In the blister floors, weakly stained linear or granular deposits of some of these components were sometimes also present. The results of this study support discontinuity of the basement membrane as the main anomaly in delayed postburn blistering. Disturbance in the reassembly or local breakdown of the basement membrane components might be the underlying defect. PMID:9274961

  14. Blistering during the atomic layer deposition of iridium

    Energy Technology Data Exchange (ETDEWEB)

    Genevée, Pascal, E-mail: pascal-genevee@chimie-paristech.fr, E-mail: a.szeghalmi@uni-jena.de; Ahiavi, Ernest; Janunts, Norik; Pertsch, Thomas; Kley, Ernst-Bernhard; Szeghalmi, Adriana, E-mail: pascal-genevee@chimie-paristech.fr, E-mail: a.szeghalmi@uni-jena.de [Institut für Angewandte Physik, Friedrich-Schiller-Universität Jena, Max-Wien-Platz 1, 07743 Jena (Germany); Oliva, Maria [Fraunhofer IOF, Albert-Einstein-Strasse 7, 07743 Jena (Germany)

    2016-01-15

    The authors report on the formation of blisters during the atomic layer deposition of iridium using iridium acetylacetonate and oxygen precursors. Films deposited on fused silica substrates led to sparsely distributed large blisters while in the case of silicon with native oxide additional small blisters with a high density was observed. It is found that the formation of blisters is favored by a higher deposition temperature and a larger layer thickness. Postdeposition annealing did not have a significant effect on the formation of blisters. Finally, changing purge duration during the film growth allowed us to avoid blistering and evidenced that impurities released from the film in gas phase were responsible for the formation of blisters.

  15. Blisters - an unusual effect during radiotherapy.

    Science.gov (United States)

    Höller, U; Schubert, T; Budach, V; Trefzer, U; Beyer, M

    2013-11-01

    The skin reaction to radiation is regularly monitored in order to detect enhanced radiosensitivity of the patient, unexpected interactions (e.g. with drugs) or any inadvertent overdosage. It is important to distinguish secondary disease from radiation reaction to provide adequate treatment and to avoid unnecessary discontinuation of radiotherapy. A case of bullous eruption or blisters during radiotherapy of the breast is presented. Differential diagnoses bullous pemphigoid, pemphigus vulgaris, and bullous impetigo are discussed and treatment described. PMID:24158604

  16. How do keratinizing disorders and blistering disorders overlap?

    Science.gov (United States)

    Hamada, Takahiro; Tsuruta, Daisuke; Fukuda, Shunpei; Ishii, Norito; Teye, Kwesi; Numata, Sanae; Dainichi, Teruki; Karashima, Tadashi; Ohata, Chika; Furumura, Minao; Hashimoto, Takashi

    2013-02-01

    Inherited keratinizing disorders are caused by mutations in the genes encoding cornified cell envelope proteins, enzymes and their inhibitors, adhesion molecules, cytoskeletal proteins and others in the epidermis. These molecules are known to regulate differentiation, proliferation and cell adhesions. Intriguingly, some keratinizing disorders show blistering skin lesions, while some inherited blistering disorders show abnormal keratinization. Therefore, hereditary keratinizing and blistering diseases are closely related and show overlapping genetic backgrounds. In this review, we overviewed keratinizing and blistering disorders in terms of overlapping of the two disease groups. Gene mutations in desmosomal components cause striate keratoderma, Naxos disease, epidermolytic palmoplantar keratoderma and plakophilin deficiency, which first show skin fragility and blisters and later hyperkeratosis. Gene mutations in hemidesmosomal components cause various forms of epidermolysis bullosa, some of which show hyperkeratosis on the nails, palms and soles, in addition to blister formation. Diseases with gene mutations in calcium pump proteins are Darier disease and Hailey-Hailey disease, which show clinicopathological overlaps and develop both keratinizing and blistering skin lesions. Finally, gene mutations in epidermal keratins cause epidermolysis bullosa simplex, epidermolytic ichthyosis, superficial epidermolytic ichthyosis, epidermolytic palmoplantar keratoderma and pachyonychia congenita/focal palmoplantar keratoderma, which show thickening of the palms and soles with underlying blister formation. In general, responsible proteins for diseases developing both keratinizing and blistering conditions are adhesion molecules, calcium pump proteins and keratins, but not connexins, cornified cell envelop proteins, enzymes or inhibitors. It is still unknown how particular keratinizing diseases develop blisters and vice versa.

  17. Inhibition of Rho A activity causes pemphigus skin blistering

    OpenAIRE

    Waschke, Jens; Spindler, Volker; Bruggeman, Paola; Zillikens, Detlef; Schmidt, Gudula; Drenckhahn, Detlev

    2006-01-01

    The autoimmune blistering skin diseases pemphigus vulgaris (PV) and pemphigus foliaceus (PF) are mainly caused by autoantibodies against desmosomal cadherins. In this study, we provide evidence that PV–immunoglobulin G (IgG) and PF-IgG induce skin blistering by interference with Rho A signaling. In vitro, pemphigus IgG caused typical hallmarks of pemphigus pathogenesis such as epidermal blistering in human skin, cell dissociation, and loss of desmoglein 1 (Dsg 1)–mediated binding probed by la...

  18. Reliable site for suction blister induction and harvesting

    Directory of Open Access Journals (Sweden)

    Laxmisha Chandrashekar

    2005-01-01

    Full Text Available Background: Suction blister grafting is a useful modality of treatment of patients with resistant and stable vitiligo. However, there have been no detailed studies to find out the best donor site for blister formation. Methods: The study was conducted between the period of October 2004 and February 2005 in the dermatology department at a tertiary care center. Nine patients with vitiligo (focal vitiligo, 3; mucosal vitiligo, 2; acrofacial vitiligo, 2; vitiligo vulgaris, 1; and segmental vitiligo, 1 were selected for blister harvesting and grafting. The blisters were raised using the method described by Gupta et al. Results: Suction blisters were attempted to be raised at 52 sites, but only 38 blisters could be raised, 24 complete and 14 incomplete. Blisters were raised in all the three cases on the flexor aspect of the arm (100%, 15 of 17 cases (88.2% on the flexor aspect of the forearm, 4 of 5 cases (80% on the abdomen, 11 of 16 cases (68.7% on the anterolateral thigh, and less frequently over leg or foot. Complete blisters were formed in 13/15 cases (86.6% on the flexor aspect of the forearm, 6/11 cases (54.5% on the anterolateral thigh, and in all cases over leg. Conclusion: The flexor aspect of the forearm is a good site for suction blister harvesting.

  19. MYCETOMA: AN UNUSUAL MANIFESTATION OF MULTIPLE BLISTERS

    Directory of Open Access Journals (Sweden)

    Shyamala

    2014-08-01

    Full Text Available Four cases of Mycetoma especially among farmers were confirmed both by demonstrating granules in histopathological sections and by isolating Madurellagrisea in two patients, Madurellamycetomatis in one and Nocardia asteroids in one, following retrieval trauma. Eumycotic and Actinomycotic agents produce severe morbidity. One cases of Actinomycoticmycetoma responded to Trimethoprim-Sulphamethasone and the remaining three had antifungal Ketaconazole therapy for six months and did not return up for further follow up. These four cases are being reported here for its unusual clinical manifestations of multiple blisters in the extremities without the sinus tract formation and clinically mycetoma could not be diagnosed and indicates the need for biopsy examination and mycology culture.

  20. Rituximab for autoimmune blistering diseases: recent studies, new insights

    OpenAIRE

    Lunardon, Luisa; Payne, Aimee S.

    2012-01-01

    Rituximab, an anti-CD20 monoclonal antibody, has been successfully used off-label for treatment of autoimmune blistering diseases. We discuss rituximab mechanisms of action, host factors that may affect response to rituximab, and the efficacy and safety of rituximab in autoimmune blistering diseases, incorporating recent data on the use of rituximab in other autoimmune disease patients.

  1. Inspection procedures for assessment of blister formation/cracking

    International Nuclear Information System (INIS)

    There are various types of inspection that can provide information about a pressure tube's condition with respect to blister formation and cracking. These include spacer location, pressure tube/calandria tube (PT/CT) gap measurement, detection of PT/CT contact, detection of cracked blisters, detection of uncracked blisters, measurement of hydrogen by taking scrape samples and measurement of hydrogen using non-destructive methods. Detection of PT/CT contact and detection of cracked and uncracked blisters are performed using ultrasonic methods. India has developed an ultrasonic inspection capability,/1/, but it has been operated at what we believe is too low a sensitivity,/2/. Current ultrasonic methods and experience related to contact and blister detection are described. 13 refs., 11 figs

  2. Application of the Blister Test in Study of Epoxy Adhesive

    Institute of Scientific and Technical Information of China (English)

    Fei Xiong; Ingegerd Annergren

    2000-01-01

    Shaft-loaded blister test technique is used as an effective quantitative tool to measure adhesion strength. Investigation on conductive adhesive was done by modified blister test. It is found that shaftloaded blister test can be a good solution for the debonding of thin film adhesion. The intrinsic stable interface debonding process has been proved an attractive alternative to the conventional adhesion measurement techniques. In our study, epoxy matrix adhesive was studied using blister test technique in comparison with the traditional test-lap shear test. Adhesion strength was studied as a function of surface treatment and the metallization of substrate. It was found that surface conditions of substrate have significant impact on adhesion behaviour. The oxidation of surface is responsible for the poor adhesion. Activating chemical treatment and Plasma cleaning on substrate surface has been found to be a way of dreamatically improving adhesion strength of electronic conductive adhesive.

  3. Suction blister grafting - Modifications for easy harvesting and grafting

    Directory of Open Access Journals (Sweden)

    2012-01-01

    Full Text Available Suction blister grafting is a simple modality of treatment of patients with resistant and stable vitiligo. But raising the blisters may be time consuming and transferring to the recipient site may be difficult as the graft is ultrathin. By doing some modifications we can make the technique simpler and easier. We can decrease the blister induction time by intradermal injection of saline, exposure to Wood′s lamp, intrablister injection of saline. By these methods we can decrease the blister induction time from 2-3 hrs to 45-90 minutes. After harvesting the graft, it can be transferred to the recipient area by taking the graft on a sterile glass slide, on the gloved finger, rolling the graft over a sterile syringe and then spreading on the recipient area, or taking on the sterile wrapper of paraffin dressing and then placing over the recipient area.

  4. The ‘Sticky Elastica’: delamination blisters beyond small deformations

    KAUST Repository

    Wagner, Till J. W.

    2013-01-01

    We consider the form of an elastic loop adhered to a rigid substrate: the \\'Sticky Elastica\\'. In contrast to previous studies of the shape of delamination \\'blisters\\', the theory developed accounts for deflections with large slope (i.e. geometrically nonlinear). Starting from the classical Euler Elastica we provide numerical results for the dimensions of such blisters for a variety of end-end confinements and develop asymptotic expressions that reproduce these results well, even up to the point of self-contact. Interestingly, we find that the width of such blisters does not grow monotonically with increased confinement. Our theoretical predictions are confirmed by simple desktop experiments and suggest a new method for the measurement of the elastocapillary length for deformations that cannot be considered small. We discuss the implications of our results for applications such as flexible electronics. © 2013 The Royal Society of Chemistry.

  5. U-Mo Plate Blister Anneal Interim Report

    Energy Technology Data Exchange (ETDEWEB)

    Francine J. Rice; Daniel M. Wachs; Adam B. Robinson; Dennis D. Keiser Jr.; Jan-Fong Jue; Danielle M. Perez; Ross Finlay

    2010-10-01

    Blister thresholds in fuel elements have been a longstanding performance parameter for fuel elements of all types. This behavior has yet to be fully defined for the RERTR U-Mo fuel types. Blister anneal studies that began in 2007 have been expanded to include plates from more recent RERTR experiments. Preliminary data presented in this report encompasses the early generations of the U-Mo fuel systems and the most recent but still developing fuel system. Included is an overview of relevant dispersion fuel systems for the purposes of comparison.

  6. Mechanism for orientation dependence of blisters on W surface exposed to D plasma at low temperature

    Science.gov (United States)

    Jia, Y. Z.; Liu, W.; Xu, B.; Luo, G.-N.; Qu, S. L.; Morgan, T. W.; De Temmerman, G.

    2016-08-01

    The orientation dependence of blister formation induced by D plasma exposure at low temperature (about 523 K) on rolled tungsten and chemical vapor deposition (CVD) W samples was studied by scanning electron microscopy and electron backscatter diffraction. Severe blistering was observed on grains with surface normal directions close to [111], while the [001] surfaces are the most resistant to blister formation. Cavities induced by D2 gas were observed beneath [111], [110] and [001] surfaces, independently on whether blisters were observed on the surface or not. The [111] surface is more prone to blister formation, because it is easily plastically deformed by the D2 gas pressure. Some blister edges and steps were perpendicular to [110] directions, which may be induced by the slipping of dislocations on {110} planes. The blister morphology induced by D plasma can be well explained by the blister model based on plastic deformation mechanism.

  7. Pharmacokinetics of cefetamet in plasma and skin blister fluid.

    OpenAIRE

    Zimmerli, W; Sansano, S; Wittke, B

    1996-01-01

    Cefetamet pivoxil is an oral cephalosporin with enhanced affinity for the target penicillin-binding proteins 1 and 3 and an increased stability to beta-lactamases compared with older cephalosporins, such as cefalexin or cefaclor. The pharmacokinetics of cefetamet pivoxil was determined after the seventh and final dose of 500 mg of cefetamet pivoxil in eight healthy volunteers. Concentrations in plasma and cantharidin-induced skin blister fluid were determined by a high-performance liquid chro...

  8. Inhibition of Rho A activity causes pemphigus skin blistering

    Science.gov (United States)

    Waschke, Jens; Spindler, Volker; Bruggeman, Paola; Zillikens, Detlef; Schmidt, Gudula; Drenckhahn, Detlev

    2006-01-01

    The autoimmune blistering skin diseases pemphigus vulgaris (PV) and pemphigus foliaceus (PF) are mainly caused by autoantibodies against desmosomal cadherins. In this study, we provide evidence that PV–immunoglobulin G (IgG) and PF-IgG induce skin blistering by interference with Rho A signaling. In vitro, pemphigus IgG caused typical hallmarks of pemphigus pathogenesis such as epidermal blistering in human skin, cell dissociation, and loss of desmoglein 1 (Dsg 1)–mediated binding probed by laser tweezers. These changes were accompanied by interference with Rho A activation and reduction of Rho A activity. Pemphigus IgG–triggered keratinocyte dissociation and Rho A inactivation were p38 mitogen-activated protein kinase dependent. Specific activation of Rho A by cytotoxic necrotizing factor-y abolished all pemphigus-triggered effects, including keratin retraction and release of Dsg 3 from the cytoskeleton. These data demonstrate that Rho A is involved in the regulation of desmosomal adhesion, at least in part by maintaining the cytoskeletal anchorage of desmosomal proteins. This may open the possibility of pemphigus treatment with the epidermal application of Rho A agonists. PMID:17130286

  9. Mechanics of tungsten blistering II: Analytical treatment and fracture mechanical assessment

    Science.gov (United States)

    Li, Muyuan; You, Jeong-Ha

    2015-10-01

    Since a decade the blistering of pure tungsten under hydrogen implantation has been one of the major research topics in relation to the plasma-wall interaction of tungsten-armored first wall. Overall blistering may reduce the erosion lifetime of the wall. Mature blisters grown by high internal pressure are likely to burst leading to exfoliation of the surface. Therefore, the control and suppression of blistering is an important concern for sustainable operation of the tungsten-armored plasma-facing components. In this context, a quantitative assessment of the mechanical conditions for blister bulging and growth is an important concern. In this article a theoretical framework is presented to describe the bulging deformation of tungsten blisters and to estimate the mechanical driving force of blister growth. The validity of the analytical formulations based on the theory of elastic plates is evaluated with the help of finite element analysis. Plastic strains and J-integral values at the blister boundary edge are assessed by means of numerical simulation. Extensive parametric studies were performed for a range of blister geometry (cap aspect ratio), gas pressure, yield stress and hardening rate. The characteristic features of the blistering mechanics are discussed and the cracking energy is quantitatively estimated for the various combinations of parameters.

  10. Multi-scale characterization of surface blistering morphology of helium irradiated W thin films

    Energy Technology Data Exchange (ETDEWEB)

    Yang, J.J., E-mail: jjyang@scu.edu.cn [Key Laboratory of Radiation Physics and Technology of Ministry of Education, Institute of Nuclear Science and Technology, Sichuan University, Chengdu, Sichuan 610064 (China); Zhu, H.L. [Key Laboratory of Radiation Physics and Technology of Ministry of Education, Institute of Nuclear Science and Technology, Sichuan University, Chengdu, Sichuan 610064 (China); Wan, Q. [Institute of Structural Mechanics, China Academy of Engineering Physics, Mianyang, Sichuan 621900 (China); Peng, M.J. [Key Laboratory of Radiation Physics and Technology of Ministry of Education, Institute of Nuclear Science and Technology, Sichuan University, Chengdu, Sichuan 610064 (China); Ran, G., E-mail: gran@xmu.edu.cn [School of Energy Research, Xiamen University, Xiamen, Fujian 361005 (China); Tang, J.; Yang, Y.Y.; Liao, J.L.; Liu, N. [Key Laboratory of Radiation Physics and Technology of Ministry of Education, Institute of Nuclear Science and Technology, Sichuan University, Chengdu, Sichuan 610064 (China)

    2015-09-01

    Highlights: • Multi-scale blistering morphology of He irradiated W film was studied. • This complex morphology was first characterized by wavelet transform approach. - Abstract: Surface blistering morphologies of W thin films irradiated by 30 keV He ion beam were studied quantitatively. It was found that the blistering morphology strongly depends on He fluence. For lower He fluence, the accumulation and growth of He bubbles induce the intrinsic surface blisters with mono-modal size distribution feature. When the He fluence is higher, the film surface morphology exhibits a multi-scale property, including two kinds of surface blisters with different characteristic sizes. In addition to the intrinsic He blisters, film/substrate interface delamination also induces large-sized surface blisters. A strategy based on wavelet transform approach was proposed to distinguish and extract the multi-scale surface blistering morphologies. Then the density, the lateral size and the height of these different blisters were estimated quantitatively, and the effect of He fluence on these geometrical parameters was investigated. Our method could provide a potential tool to describe the irradiation induced surface damage morphology with a multi-scale property.

  11. Multi-scale characterization of surface blistering morphology of helium irradiated W thin films

    International Nuclear Information System (INIS)

    Highlights: • Multi-scale blistering morphology of He irradiated W film was studied. • This complex morphology was first characterized by wavelet transform approach. - Abstract: Surface blistering morphologies of W thin films irradiated by 30 keV He ion beam were studied quantitatively. It was found that the blistering morphology strongly depends on He fluence. For lower He fluence, the accumulation and growth of He bubbles induce the intrinsic surface blisters with mono-modal size distribution feature. When the He fluence is higher, the film surface morphology exhibits a multi-scale property, including two kinds of surface blisters with different characteristic sizes. In addition to the intrinsic He blisters, film/substrate interface delamination also induces large-sized surface blisters. A strategy based on wavelet transform approach was proposed to distinguish and extract the multi-scale surface blistering morphologies. Then the density, the lateral size and the height of these different blisters were estimated quantitatively, and the effect of He fluence on these geometrical parameters was investigated. Our method could provide a potential tool to describe the irradiation induced surface damage morphology with a multi-scale property

  12. DOSE MORTALITY RESPONCES OF BLISTER BEETLES AGAINST SOME INSECTICIDES

    Directory of Open Access Journals (Sweden)

    SARIKA P. SHENDE

    2013-01-01

    Full Text Available In view of significant damage potential of adult blister beetles, dose mortality response for number of insecticideswas evaluated to find out efficacious alternative for management of blister beetles. The experiments were conductedat Insect Toxicology Laboratory, Department of Agricutural Entomology, Dr. Panjabrao Deshmukh KrishiVidyapeeth, Akola (Maharashtra. The insecticides viz., chlorpyriphos 50 EC + cypermethrin 5 EC (0.1375%,lambda cyhalothrin 2.5 EC (0.00375%, alphamethrin 20 EC (0.05%, cypermethrin 10 EC (0.01%, fenvalerate20 EC (0.02% and deltamethrin 2.8 EC (0.0042% at field dose inflicted 100 per cent mortality, within 24 hours.The data on relative toxicity revealed that lambda cyhalothrin 2.5 EC, deltamethrin 2.8 EC and cypermethrin 10EC were 188.3, 113.0 and 56.5 times more toxic with reference to chlorpyriphos dust, respectively. The order oftoxicity based on LC50 values for insecticides under evaluation was lambda cyhalothrin (0.06 ppm > deltamethrin(0.1 ppm > cypermethrin (0.2 ppm > alphamethrin (0.4 ppm > fenvalerate (0.6 ppm > chlorpyriphos +Cypermethrin (0.8 ppm > methyl parathion dust (1.1 ppm > fenvalerate dust (3.0 ppm > chlorpyriphos dust(11.3 ppm. Lower LT50 values at 103 dilutions was recorded in cypermethrin (10.0h and chorpyriphos +cypermethrin (13.0h. In case of dust formulations, minimum LT50 value was recorded with methyl parathion(83.1h, whereas, fenvalerate dust (109.2h registered highest value. Similar, efficacy trend based on LT50 valueswas recorded at 104 dilutions. Synthetic pyrethroids proved efficient in inflicting mortality quickly and at lowerdose, thus, suggesting their potential in management of blister beetles.

  13. Modeling the Photoionized Interface in Blister HII Regions

    CERN Document Server

    Sankrit, R; Sankrit, Ravi

    2000-01-01

    We present a grid of photoionization models for the emission from photoevaporative interfaces between the ionized gas and molecular cloud in blister HII regions. For the density profiles of the emitting gas in the models, we use a general power law form calculated for photoionized, photoevaporative flows by Bertoldi (1989). We find that the spatial emission line profiles are dependent on the incident flux, the shape of the ionizing continuum and the elemental abundances. In particular, we find that the peak emissivity of the [SII] and [NII] lines are more sensitive to the elemental abundances than are the total line intensities. The diagnostics obtained from the grid of models can be used in conjunction with high spatial resolution data to infer the properties of ionized interfaces in blister HII regions. As an example, we consider a location at the tip of an ``elephant trunk'' structure in M16 (the Eagle Nebula) and show how narrow band HST-WFPC2 images constrain the HII region properties. We present a photo...

  14. Hydrides blister formation and induced embrittlement on zircaloy-4 cladding tubes in reactivity initiated conditions

    International Nuclear Information System (INIS)

    Our aim is to study the cladding fracture with mechanical tests more representative of RIA conditions, taking into account the hydrides blisters, representative strain rates and stress states. To obtain hydride blisters, we developed a thermodiffusion setup that reproduces blister growth in reactor conditions. By metallography, nano-hardness, XRD and ERDA, we showed that they are constituted by 80% to 100% of δ hydrides in a Zircaloy-4 matrix, and that the zirconium beneath has some radially oriented hydrides. We modeled the blister growth kinetics taking into account the hysteresis of the hydrogen solubility limit and defined the thermal gradient threshold for blister growth. The modeling of the dilatometric behavior of hydrided zirconium indicates the important role of the material crystallographic texture, which could explain differences in the blister shape. Mechanical tests monitored with an infrared camera showed that significant local heating occurred at strain rates higher than 0.1/s. In parallel, the Expansion Due to Compression test was optimized to increase the bi-axiality level from uniaxial stress to plane strain (HB-EDC and VHB-EDC tests). This increase in loading bi-axiality lowers greatly the fracture strain at 25 C and 350 C only in homogeneous material without blister. Eventually, the ductility decrease of unirradiated Zircaloy-4 cladding tube in function of the blister depth was quantified. (author)

  15. Influence of hydrogen fluence on surface blistering of H and He co-implanted Ge

    Science.gov (United States)

    Dai, Jiayun; Xue, Zhongying; Zhang, Miao; Wei, Xing; Wang, Gang; Di, Zengfeng

    2016-02-01

    The effect of hydrogen fluence on surface blistering of H and He co-implanted Ge is investigated using atom force microscope, X-ray diffraction and transmission electron microscopy. With a fixed He, we find that for 1 × 1016 cm-2 H implantation fluence, only a few small dome-shaped blisters appear, for 3 × 1016 cm-2 H implantation fluence, large blisters as well as craters are formed, while for 5 × 1016 cm-2 H implantation fluence, no blisters can be observed. The strain evolution and platelet forming tendency are found to be relevant for the different blistering phenomenon. The weak blistering phenomenon for 1 × 1016 cm-2 H implantation fluence may be attributed to less "free" H for the building up of internal pressure of platelets and the sustained growth of platelets. While the absence of blistering phenomenon for 5 × 1016 cm-2 H implantation fluence is likely due to the retarded relief of the decreased uniform compressive stress throughout the damage region.

  16. A novel investigation of a blister-like syndrome in aquarium Echinopora lamellosa.

    Directory of Open Access Journals (Sweden)

    David Smith

    Full Text Available This study investigates potential causes of a novel blister-like syndrome in the plating coral Echinopora lamellosa. Visual inspections of this novel coral syndrome showed no obvious signs of macroparasites and the blisters themselves manifested as fluid-filled sacs on the surface of the coral, which rose from the coenosarc between the coral polyps. Histological analysis of the blisters showed that there was no associated necrosis with the epidermal or gastrodermal tissues. The only difference between blistered areas and apparently healthy tissues was the presence of proliferated growth (possible mucosal cell hyperplasia directly at the blister interface (area between where the edge of the blister joined apparently healthy tissue. No bacterial aggregates were identified in any histological samples, nor any sign of tissue necrosis identified. We conclude, that the blister formations are not apparently caused by a specific microbial infection, but instead may be the result of irritation following growth anomalies of the epidermis. However, future work should be conducted to search for other potential casual agents, including viruses.

  17. Combined effects of crystallography, heat treatment and surface polishing on blistering in tungsten exposed to high-flux deuterium plasma

    Science.gov (United States)

    Zayachuk, Y.; Tanyeli, I.; Van Boxel, S.; Bystrov, K.; Morgan, T. W.; Roberts, S. G.

    2016-08-01

    For tungsten exposed to low-energy hydrogen-plasmas, it has been thought that grains with surface normal are most susceptible to blistering while those with surface normal are virtually impervious to it. Here, we report results showing that non-uniformity of blister distribution depends on the state of the surface due to polishing. In electrochemically polished material blisters appear on the grains with all orientations, while in mechanically polished material blister-free areas associated with particular orientations emerge. On the other hand, blistering is shown to have a strong dependence on the level of deformation within particular grains in partially recrystallized material.

  18. Synergistic helium and deuterium blistering in tungsten–tantalum composites

    Energy Technology Data Exchange (ETDEWEB)

    Dias, M., E-mail: marta.dias@itn.pt [Associação Euratom/IST, Instituto de Plasmas e Fusão Nuclear, Instituto Superior Técnico, Universidade de Lisboa, Av. Rovisco Pais, 1049-001 Lisboa (Portugal); Mateus, R.; Catarino, N.; Franco, N. [Associação Euratom/IST, Instituto de Plasmas e Fusão Nuclear, Instituto Superior Técnico, Universidade de Lisboa, Av. Rovisco Pais, 1049-001 Lisboa (Portugal); Nunes, D. [CENIMAT-I3N, Departamento de Ciência dos Materiais, Faculdade de Ciências e Tecnologia, FCT, Universidade Nova de Lisboa, 2829-516 Caparica (Portugal); Correia, J.B. [LNEG, Laboratório Nacional de Energia e Geologia, Estrada do Paço do Lumiar, 1649-038 Lisboa (Portugal); Carvalho, P.A. [Associação Euratom/IST, Instituto de Plasmas e Fusão Nuclear, Instituto Superior Técnico, Universidade de Lisboa, Av. Rovisco Pais, 1049-001 Lisboa (Portugal); ICEMS, Instituto Superior Técnico, Universidade de Lisboa, Av. Rovisco Pais, 1049-001 Lisboa (Portugal); Hanada, K. [AIST, National Institute of Advanced Industrial Science and Technology, 1-2-1 Namiki, Tsukuba, 305-8564 Ibaraki (Japan); Sârbu, C. [National Institute of Materials and Physics, 105bis Atomistilor street, 077125 Magurele-Ilfov (Romania); and others

    2013-11-15

    Abstruct: Tungsten–tantalum composites with 10 and 20 at.% Ta were prepared by ball milling W powder with Ta fibers and by consolidating the milled materials with spark plasma sintering. The composites were implanted at room temperature with He{sup +} (30 keV with a fluence 5 × 10{sup 21} at/m{sup 2}) and/or D{sup +} (15 keV with a fluence 5 × 10{sup 21} at/m{sup 2}) ion beams. The materials were studied by scanning and high-resolution transmission electron microscopy, both coupled with energy dispersive X-ray spectroscopy, and by X-ray diffraction, Rutherford backscattering spectrometry and nuclear reaction analysis. The microstructure observations revealed that the milling operation resulted in severe fragmentation of the Ta fibers. Furthermore, during the consolidation process the Ta phase acted as oxygen getter and reduced the W oxide present in the original material. The surface of the tungsten–tantalum composites implanted with D{sup +} remained essentially unaltered, while the materials implanted with He{sup +} evidenced blisters on the Ta-rich regions. D retention in the composites increased with He{sup +} pre-implantation.

  19. The formation and characteristics of hydride blisters in c.w. Zircaloy-2 pressure tubes

    International Nuclear Information System (INIS)

    Under the auspices of the IAEA, a consultants' meeting was arranged in Vienna, 1994 July 25-29, at which a Canadian delegation, consisting of AECL and Ontario Hydro Technologies personnel, presented information on their knowledge of the behaviour of hydride blisters in Zircaloy-2 pressure tubes. This document contains the 10 papers presented by the Canadian delegation to the meeting. It is believed that they represent a good reference document on hydride blister phenomena

  20. Study on blister of the coating on solid cantilevers of hydraulic supports for coal mining

    Institute of Scientific and Technical Information of China (English)

    2010-01-01

    In this work, blister of the Cu-Sn plus Cr coating on solid cantilevers of hydraulic supports for coal mining was investigated by hydrogen-charging, Devanathan-Stachurski method and electrochemical impedance spectroscopy (EIS) measurement. It was found that the permeation hydrogen during the pickling process and the electroplating process was responsible for the blisters. The residual tensile stress due to the machining process would increase the permeation hydrogen amount during pickling and electroplating processes.

  1. Autoimmune blistering diseases of the pemphigus group = Enfermedades ampollosas autoinmunes del grupo de los pénfigos

    Directory of Open Access Journals (Sweden)

    Juliana Calle Isaza

    2014-07-01

    Full Text Available Autoimmune blistering diseases of the pemphigus group Blisters may appear in many dermatological diseases, but they are not necessarily of autoimmune etiology. For the study of blistering diseases, it is necessary to take into account the clinical aspects, the history of when and how blisters appeared, the epidemiological and histological information (for instance, the skin level at which blisters are located, and whether inflammatory infiltrates are present. In order to corroborate the autoimmune etiology of blisters, it is important to have the results of confirmatory tests such as direct and indirect immunofluorescence, immune blotting, enzyme-linked immune-assay (ELISA, immune precipitation, and electronic microscopy. Information on autoantibodies serum titers may help to conduct a more precise immunosuppressive therapy.

  2. An unusual case of duodenal perforation caused by a blister pack: A case report and literature review

    Directory of Open Access Journals (Sweden)

    Si-Yuan Yao

    2015-01-01

    Conclusion: Unnoticed ingestion of blister packs can cause duodenal perforation. Although prompt management is necessary, duodenal perforation, especially in the third portion, may be potentially fatal.

  3. IMMUNOHISTOCHEMISTRY VERSUS IMMUNOFLUORESENCE IN THE DIAGNOSIS OF AUTOIMMUNE BLISTERING DISEASES

    Directory of Open Access Journals (Sweden)

    Ana Maria Abreu Velez

    2013-11-01

    Full Text Available Introduction: In patients with autoimmune skin blistering diseases (ABDs, the diagnostic gold standard has classically been direct and indirect immunofluorescence (DIF and IIF, despite inherent technical problems of autofluorescence. Aim: We sought to overcome autofluorescence issues and compare the reliability of immunofluorescence versus immunohistochemistry (IHC staining in the diagnoses of these diseases. Methods: We tested via IHC for anti-human IgG, IgM, IgA, IgD, IgE, Kappa light chains, Lambda light chains, Complement/C3c, Complement/C1q, Complement/C3d, albumin and fibrinogen in 30 patients affected by a new variant of endemic pemphigus foliaceus in El Bagre, Colombia (El Bagre-EPF, and 30 control biopsies from the endemic area. We also tested archival biopsies from patients with ABDs whose diagnoses were made clinically, histopathologically and by DIF/IIF studies from 2 independent dermatopathology laboratories in the USA. Specifically, we tested 34 patients with bullous pemphigoid (BP, 18 with pemphigus vulgaris (PV, 8 with pemphigus foliaceus (PF, 14 with dermatitis herpetiformis (DH and 30 control skin samples from plastic esthetic surgery reduction surgeries. Results: The diagnostic correlation between IHC and DIF-IIF was almost 98% in most cases. IHC revealed evidence of autofluorescence around dermal blood vessels, dermal eccrine glands and neurovascular packages feeding skin appendices in ABDs; this autofluorescence may represent a non-specific immune response. Strong patterns of positivity were seen also in endothelial-mesenchymal cell junction-like structures, as well as between dermal fibrohistiocytic cells. In PV, we noted strong reactivity to neurovascular packages supplying sebaceous glands, as well as apocrine glands with edematous changes. Conclusions: We suggest that IHC is as reliable as DIF or IIF for the diagnosis of ABDs; our findings further suggest that what has previously been considered DIF/IIF autofluorescence

  4. Etiological Foundation for Practical Strategies to Prevent Exercise-Related Foot Blisters.

    Science.gov (United States)

    Hoffman, Martin D

    2016-01-01

    This work outlines the etiological factors for exercise-related foot blisters and the pertinent prevention strategies related to these causes. Blisters result from shear forces within the epidermis causing cell necrosis. The extent of skin shear is influenced by friction at the skin and other interfaces, various skin characteristics, bony movement, and the shear modulus of the foot ware. The number of shear cycles is another factor in the development of blisters. Key preventative strategies include limiting the number of shear cycles, avoiding moisture and particulate accumulation next to the skin, frequent use of skin lubricants, elimination of pressure points through proper fitting and broken in shoes and callous removal, use of low shear modulus insoles, and induction of skin adaptations through proper training. Other methods requiring further research, but with theoretical support, include the use of taping and low friction patches over high-friction areas, and double-layered or toe socks.

  5. Hydrogen blisters on beta-NbD after laser pulse heating

    Science.gov (United States)

    Schober, T.; Bechthold, P. S.

    1994-08-01

    Plates of beta-NbD(0.7) were irradiated with single pulses of a KrF excimer laser having a wavelength of 248.4 nm. The power level was varied from roughly 0.1 to 1 GW/sq cm. The pulse length was about 15 ns. The irradiated areas at low and medium power displayed individual hydrogen (deuterium) blisters which started overlapping at higher power levels. Calculation of the thermal diffusivity showed that a surface layer roughly 0.6 micrometers thick was heated during the pulse. Applying Bechtel's formula for the temperature increase in the heated layer demonstrated that melting of the heated layer must have occurred during the pulse. The formation of hydrogen (deuterium) blisters is understandable in terms of the very high equilibrium vapor pressure of the hydrides near the melting temperature. A model for the formation of the blisters is presented.

  6. The biochemistry of blister fluid from pediatric burn injuries: proteomics and metabolomics aspects.

    Science.gov (United States)

    Zang, Tuo; Broszczak, Daniel A; Broadbent, James A; Cuttle, Leila; Lu, Haitao; Parker, Tony J

    2016-01-01

    Burn injury is a prevalent and traumatic event for pediatric patients. At present, the diagnosis of burn injury severity is subjective and lacks a clinically relevant quantitative measure. This is due in part to a lack of knowledge surrounding the biochemistry of burn injuries and that of blister fluid. A more complete understanding of the blister fluid biochemistry may open new avenues for diagnostic and prognostic development. Burn insult induces a highly complex network of signaling processes and numerous changes within various biochemical systems, which can ultimately be examined using proteome and metabolome measurements. This review reports on the current understanding of burn wound biochemistry and outlines a technical approach for 'omics' profiling of blister fluid from burn wounds of differing severity.

  7. Study of hydride blisters in Zr-alloy using neutron tomography

    International Nuclear Information System (INIS)

    Highlights: ► We have established neutron tomography as a potential tool for the study of blister formation. ► Location, size and hydride distribution in the vicinity of blisters has been imaged. ► Hydrogen concentration up to 25 wppm in the reconstructed images has been detected. ► Linear relation between mode of image histogram and hydrogen concentration has been found. ► Spatial in-homogeneity of hydride distribution has also been identified in tomographic images. - Abstract: Formation of hydride blisters in Zircaloy pressure tubes of pressurized heavy water reactor (PHWR) is a major life limiting factor which hinders the safe and uninterrupted operation of the reactor. Nondestructive detection and evaluation of location and size of these blisters as well as hydride distribution in the matrix surrounding them may help in damage quantification and residual life extension. In this article we present the neutron tomography studies carried out on simulated hydride blister samples grown on Zircaloy tubes. Characterization on samples having various levels of hydrogen concentrations were also carried out for quantification of the detectability of our neutron tomography system. We could identify the spatial in-homogeneity of hydride concentration present in the samples. Quantitatively hydrogen concentration difference up to 25 wppm has been observed experimentally and calibrated against image intensity in the reconstructed image. This study establishes neutron tomography as a potential non-destructive evaluation tool for the estimation of the severity of damage in the integrity of the pressure tubes and provides valuable information about kinetics of blister formation.

  8. Comparative morphological analysis of apple blister mite, Eriophyes mali Nal., a new pest in Serbia

    Directory of Open Access Journals (Sweden)

    Biljana Vidović

    2014-06-01

    Full Text Available The apple blister mite, Eriophyes mali Nalepa, 1926 (Acari: Prostigmata: Eriophyoidea, has been recently found in Serbia as a new pest of apple. The history of its research, the results of a morphological analysis and degree of infestation are presented. A comparison of the main morphological features of mites from different populations of remote geographical origin has shown that the apple blister mite from Serbia is most similar to another European population (Bulgarian [or Austrian?] while it differs from E. mali originating from the USA and New Zealand. The percentage of infestation varied from 1.6% to 87.6%, with an average of 22.4%.

  9. A blister-test apparatus for studies on the adhesion of materials used for neural electrodes.

    Science.gov (United States)

    Ordonez, Juan; Boehler, Christian; Schuettler, Martin; Stieglitz, Thomas

    2011-01-01

    A blister test apparatus has been developed, which allows a quantitative adhesion analysis of thin-film metallizations on polymers manufactured in cleanroom conditions suitable for micromachining of neural electrode arrays. The device is capable of pressurizing metallic membranes at wafer level, monitoring the pressure and the height of the developing blister while detecting the moment of delamination, allowing the calculation of the adhesion energy between metal film and polymer. The machine is designed for quantitative long-term studies of materials used in neural microelectrode arrays.

  10. Self-Sealing Shells: Blowouts and Blisters on the Surfaces of Leaky Wind-Blown-Bubbles and Supernova Remnants

    CERN Document Server

    Pittard, Julian

    2013-01-01

    Blowouts can occur when a dense shell confining hot, high pressure, gas ruptures. The venting gas inflates a blister on the surface of the shell. Here we examine the growth of such blisters on the surfaces of wind-blown-bubbles (WBBs) and supernova remnants (SNRs) due to shell rupture caused by the Vishniac instability. On WBBs the maximum relative size of the blister (R_bstall/R) is found to grow linearly with time, but in many cases the blister radius will not exceed 20 per cent of the bubble radius. Thus blowouts initiated by the Vishniac instability are unlikely to have a major effect on the global dynamics and properties of the bubble. The relative size of blisters on SNRs is even smaller than on WBBs, with blisters only growing to a radius comparable to the thickness of the cold shell of SNRs. The small size of the SNR blowouts is, however, in good agreement with observations of blisters in the Vela SNR. The difference in relative size between WBB and SNR blisters is due to the much higher speed at whic...

  11. Study of the effect of H implantation and annealing on LiTaO3 surface blistering

    Science.gov (United States)

    Ma, Changdong; Lu, Fei; Ma, Yujie

    2015-01-01

    LiTaO3 samples are implanted by 120 keV hydrogen ion with different fluences at room temperature. H+ concentration and distribution is detected using Elastic recoil detection. Experimental results show that the threshold fluence for blistering in LiTaO3 surface is 6 × 1016 ion/cm2. Surface blistering phenomenon is studied by using optical microscopy, Rutherford back scattering spectrometry, transmission electron microscopy and atomic force microscopy. Bubble growing and surface blister's dependence on annealing process is observed and analyzed. The critical internal pressure and stress of surface blistering in H+-implanted LiTaO3 is derived based on theoretical model and experimental results. Gibbs free energy and cavity critical radius are introduced to explain the blister shrink and rupture observed in the experiment.

  12. Wound healing in a suction blister model:an experimental study with special reference to healing in patients with diabetes and patients with obstructive jaundice

    OpenAIRE

    Koivukangas, V

    2004-01-01

    Abstract The expression intensities of cytokeratins and tight junction proteins were determined on re-epithelization. Experimental blister wound healing was studied in patients with diabetes mellitus and in patients with obstructive jaundice. Suction blisters were induced on healthy volunteers, and the healing blisters were biopsied at different time points. Cytokeratin expression and the tight junction proteins ZO-1 and occludin were studied immunohistochemically. Blisters were ...

  13. 46 CFR 59.15-10 - Bagged or blistered shell plates.

    Science.gov (United States)

    2010-10-01

    ... 46 Shipping 2 2010-10-01 2010-10-01 false Bagged or blistered shell plates. 59.15-10 Section 59.15... shell plates. (a) When the shell plates of cylindrical boilers which are exposed to the radiant heat of... boiler. (b) Where the shell plate is bagged due to overheating, the Officer in Charge, Marine...

  14. Determining the feasibility of objective adherence measurement with blister packaging smart technology

    NARCIS (Netherlands)

    Onzenoort, H.A. van; Neef, C.; Verberk, W.W.; Iperen, H.P. van; Leeuw, P.W. de; Kuy, P.H. van der

    2012-01-01

    PURPOSE: The results of a feasibility study of blister-pack smart technology for monitoring medication adherence are reported. METHODS: Research in the area of objective therapy compliance measurement has led to the development of microprocessor-driven systems that record the time a unit dose is rem

  15. BLISTERING AND DEGRADATION OF POLYURETHANE COATINGS UNDER DIFFERENT ACCELERATED WEATHERING TESTS. (R828081E01)

    Science.gov (United States)

    An epoxy primer with a high gloss polyurethane topcoat coating system was exposed either only in a QUV chamber or exposed in a QUV chamber and a Prohesion chamber, alternatively, in this study. AFM studies found that micro blisters formed on the coating surface after both expo...

  16. Non-destructive Quality control of tablets and blister packs by UV imaging

    DEFF Research Database (Denmark)

    Klukkert, Marten; Wu, Jian Xiong; Rantanen, Jukka;

    2016-01-01

    Quality control of tablets and its primary packing material within the manufacturing line requires analytical routines that allow monitoring of the desired product attributes with high efficiency. The aim of this study was to evaluate the suitability of multispectral UV imaging combined with mult......Quality control of tablets and its primary packing material within the manufacturing line requires analytical routines that allow monitoring of the desired product attributes with high efficiency. The aim of this study was to evaluate the suitability of multispectral UV imaging combined...... with multivariate image analysis for verification of blister pack filling, differentiation of tablets of varying composition therein, as well as detection of imprint defects and surface cracks of bulk tablets. Moreover, the influence of polymer sealing foils on tablet characterization within blister cavities...... was investigated. Several tablets of different composition were imaged either as bulk, within unsealed blister packs, or within blister packs that were manually sealed with three different types of either PVC or PCTFE foils. It was demonstrated that UV imaging is a fast and reliable technique for counting...

  17. The blistering of a viscoelastic filament of a droplet of saliva

    CERN Document Server

    Wagner, Christian; Eggers, Jens

    2009-01-01

    A fluid dynamics video of the break up of a droplet of saliva is shown. First a viscoelastic filament is formed and than the blistering of this filament is shown. Finally, a flow induced phase separation takes place nanometer sized solid fiber remains that consist out of the biopolymers.

  18. Blister Threshold Based Thermal Limits for the U-Mo Monolithic Fuel System

    Energy Technology Data Exchange (ETDEWEB)

    D. M. Wachs; I. Glagolenko; F. J. Rice; A. B. Robinson; B. H. Rabin; M. K. Meyer

    2012-10-01

    Fuel failure is most commonly induced in research and test reactor fuel elements by exposure to an under-cooled or over-power condition that results in the fuel temperature exceeding a critical threshold above which blisters form on the plate. These conditions can be triggered by normal operational transients (i.e. temperature overshoots that may occur during reactor startup or power shifts) or mild upset events (e.g., pump coastdown, small blockages, mis-loading of fuel elements into higher-than-planned power positions, etc.). The rise in temperature has a number of general impacts on the state of a fuel plate that include, for example, stress relaxation in the cladding (due to differential thermal expansion), softening of the cladding, increased mobility of fission gases, and increased fission-gas pressure in pores, all of which can encourage the formation of blisters on the fuel-plate surface. These blisters consist of raised regions on the surface of fuel plates that occur when the cladding plastically deforms in response to fission-gas pressure in large pores in the fuel meat and/or mechanical buckling of the cladding over damaged regions in the fuel meat. The blister temperature threshold decreases with irradiation because the mechanical properties of the fuel plate degrade while under irradiation (due to irradiation damage and fission-product accumulation) and because the fission-gas inventory progressively increases (and, thus, so does the gas pressure in pores).

  19. Analysis of Heavy Metals in Blister Pack and Medical Gloves Samples

    Directory of Open Access Journals (Sweden)

    Monika Martišiūtė

    2013-10-01

    Full Text Available Incineration is a common method to treat medical wastes. Whereas pathogens and environmentally problematic organic compounds are destroyed, toxic heavy metals cannot be ruined. In this study heavy metal concentrations of two types of medical wastes were analyzed. Four different blister pack layers (polyvinyl chloride (PVC, polyvinyl chloride/polyvinylidiene chloride with pigment (PVC/PVdCp, polyvinyl chloride/polyvinylidiene chloride transparent (PVC/PVdCt and polyethylene (PE and blister pack as a unit, and three types of medical gloves (latex powdered and powder-free medical gloves, nitrile powder-free medical gloves and vinyl powdered and powder-free medical gloves were digested using high pressure digestion methods. Different acid mixtures were used to digest the samples. Heavy metal concentrations were analyzed by the inductively coupled plasma – optical emission spectrometry (ICP-OES. As, Fe, Na, P, Sn, Sr and Zn were detected in blister pack and different blister pack layers. Ca, Fe, K, Mg, Na, P, Sr and Zn were detected in three different types of medical gloves. No environmentally relevant elemental concentrations were detected except for Sn and Zn.DOI: http://dx.doi.org/10.5755/j01.erem.65.3.4157

  20. Ribosomal protein s6-ps240 is expressed in lesional skin from patients with autoimmune skin blistering diseases

    Directory of Open Access Journals (Sweden)

    Ana Maria Abreu Velez

    2013-01-01

    Full Text Available Background: The in situ signaling transduction within skin biopsies from patients affected by autoimmune skin blistering diseases is not well-characterized. Aim : In autoimmune skin blistering diseases, autoantibodies seem to trigger several intracellular signaling pathways and we investigated the presence of the phosphorylated form of ribosomal protein S6-pS240 within autoimmune skin blistering diseases biopsies. Materials and Methods: We utilized immunohistochemistry to evaluate the presence of S6-pS240 in lesional skin biopsies of patients affected by autoimmune skin blistering diseases including patients with an endemic and nonendemic pemphigus foliaceus (non EPF, with bullous pemphigoid (BP, pemphigus vulgaris (PV, dermatitis herpetiformis (DH, and the respective controls. Results: Most autoimmune bullous skin diseases biopsies stained positive for S6-pS240 around lesional blisters, including adjacent areas of the epidermis; and within upper dermal inflammatory infiltrates, and/or mesenchymal-endothelial cell junctions within the dermis. Conclusions: We document that S6-pS240 is expressed in lesional areas of skin biopsies from patients with autoimmune skin blistering diseases, as well as on eccrine glands and piloerector muscles. Thus, the role of this molecule in autoimmune skin blistering diseases warrants further study.

  1. Cracks and blisters formed close to a silicon wafer surface by He-H co-implantation at low energy

    Science.gov (United States)

    Cherkashin, N.; Daghbouj, N.; Darras, F.-X.; Fnaiech, M.; Claverie, A.

    2015-12-01

    We have studied the effect of reducing the implantation energy towards low keV values on the areal density of He and H atoms stored within populations of blister cavities formed by co-implantation of the same fluence of He then H ions into Si(001) wafers and annealing. Using a variety of experimental techniques, we have measured blister heights and depth from the surface, diameter, areal density of the cracks from which they originate as functions of implantation energy and fluence. We show that there is a direct correlation between the diameters of the cracks and the heights of the associated blisters. This correlation only depends on the implantation energy, i.e., only on the depth at which the cracks are located. Using finite element method modeling, we infer the pressure inside the blister cavities from the elastic deformations they generate, i.e., from the height of the blisters. From this, we demonstrate that the gas pressure within a blister only depends on the diameter of the associated crack and not on its depth position and derive an analytical expression relating these parameters. Relating the pressure inside a blister to the respective concentrations of gas molecules it contains, we deduce the areal densities of He and H atoms contained within the populations of blisters. After low-energy implantations (8 keV He+, 3 keV H+), all the implanted He and H atoms contribute to the formation of the blisters. There is no measurable exo-diffusion of any of the implanted gases, in contrast to what was assumed at the state of the art to explain the failure of the Smart-Cut technology when using very low energy ion implantation for the fabrication of ultra-thin layers. Alternative explanations must be investigated.

  2. Cracks and blisters formed close to a silicon wafer surface by He-H co-implantation at low energy

    International Nuclear Information System (INIS)

    We have studied the effect of reducing the implantation energy towards low keV values on the areal density of He and H atoms stored within populations of blister cavities formed by co-implantation of the same fluence of He then H ions into Si(001) wafers and annealing. Using a variety of experimental techniques, we have measured blister heights and depth from the surface, diameter, areal density of the cracks from which they originate as functions of implantation energy and fluence. We show that there is a direct correlation between the diameters of the cracks and the heights of the associated blisters. This correlation only depends on the implantation energy, i.e., only on the depth at which the cracks are located. Using finite element method modeling, we infer the pressure inside the blister cavities from the elastic deformations they generate, i.e., from the height of the blisters. From this, we demonstrate that the gas pressure within a blister only depends on the diameter of the associated crack and not on its depth position and derive an analytical expression relating these parameters. Relating the pressure inside a blister to the respective concentrations of gas molecules it contains, we deduce the areal densities of He and H atoms contained within the populations of blisters. After low-energy implantations (8 keV He+, 3 keV H+), all the implanted He and H atoms contribute to the formation of the blisters. There is no measurable exo-diffusion of any of the implanted gases, in contrast to what was assumed at the state of the art to explain the failure of the Smart-Cut technology when using very low energy ion implantation for the fabrication of ultra-thin layers. Alternative explanations must be investigated

  3. Investigation of blister formation in sputtered Cu{sub 2}ZnSnS{sub 4} absorbers for thin film solar cells

    Energy Technology Data Exchange (ETDEWEB)

    Bras, Patrice, E-mail: patrice.bras@angstrom.uu.se [Midsummer AB, Elektronikhöjden 6, SE-17543 Järfälla, Sweden and Solid State Electronics, Angström Laboratory, Uppsala University, Box 534, SE-75121 Uppsala (Sweden); Sterner, Jan [Midsummer AB, Elektronikhöjden 6, SE-17543 Järfälla (Sweden); Platzer-Björkman, Charlotte [Solid State Electronics, Angström Laboratory, Uppsala University, Box 534, SE-75121 Uppsala (Sweden)

    2015-11-15

    Blister formation in Cu{sub 2}ZnSnS{sub 4} (CZTS) thin films sputtered from a quaternary compound target is investigated. While the thin film structure, composition, and substrate material are not correlated to the blister formation, a strong link between sputtering gas entrapment, in this case argon, and blistering effect is found. It is shown that argon is trapped in the film during sputtering and migrates to locally form blisters during the high temperature annealing. Blister formation in CZTS absorbers is detrimental for thin film solar cell fabrication causing partial peeling of the absorber layer and potential shunt paths in the complete device. Reduced sputtering gas entrapment, and blister formation, is seen for higher sputtering pressure, higher substrate temperature, and change of sputtering gas to larger atoms. This is all in accordance with previous publications on blister formation caused by sputtering gas entrapment in other materials.

  4. Reproduction in Laboratory and characterization of Blister of Hydride of zirconium in nuclear fuel pods; Reproduccion en laboratorio y caracterizacion de Blisters de hidroduro de circonio en muestras de vaina de combustible nuclear

    Energy Technology Data Exchange (ETDEWEB)

    Martin Rengel, M. A.; Ruiz-Hervias, J.; Munoz, P.

    2014-07-01

    This paper have replicated in laboratory blisters of different size in samples of pod of ZIRLO pre-hydrided evenly with 500 ppm of hydrogen. For these samples was used a technique of cathodic charging in basic medium. To produce the blister was heated up to about 350 degree centigrade in its outer surface sample. With the aim of producing a point cold on the surface of the sheath contacted the surface with a piece of aluminum water-cooled (cold finger). Was held a morphological characterization of the blisters by means of optical microscopy and found that the size of the produced blister is function of the contact time between fuel pod and cold finger. (Author)

  5. The effect of adhesive layer elasticity on the fracture mechanics of a blister test specimen

    Science.gov (United States)

    Updike, D. P.

    1975-01-01

    An analytical model of a blister type specimen for evaluating adhesive bond strength was developed. Plate theory with shear deformation was used to model the deformation of the plate, and elastic deformation of the adhesive layer is taken into account. It is shown that the inclusion of the elastic deformation of the adhesive layer can have a significant influence in the energy balance calculations of fracture mechanics.

  6. Modeling Blister Rust Incidence in Whitebark Pine at Northern Rocky Mountain Alpine Treelines: A Geospatial Approach

    OpenAIRE

    Smith, Emily Katherine

    2009-01-01

    The status of whitebark pine (Pinus albicaulis), a foundation and keystone species and a pioneer establisher at alpine treeline, is threatened by the invasive and exotic fungal pathogen (Cronartium ribicola) that causes white pine blister rust in five-needled pines. Originally thought to be limited to moderate environments, the disease is now found extensively throughout colder and dryer regions east of the Continental Divide, including alpine treeline. My research objective was to determine ...

  7. SUCCESSFUL TREATMENT OF MULTIDRUG-RESISTANT ACINETOBACTOR BAUMANII BLISTERS ON THE KNEE.

    Science.gov (United States)

    Afridi, Sikandar Aziz; Alvi, Hamid Fazeel; Lodhi, Fahad Saqib; Muhammad, Gul; Qureshi, Zaheer Uddin; Ullah, Raffat; Muhammad, Taj; Afridi, Bakhtiar Ullah

    2015-01-01

    Acinetobacter baumannii (A. baumannii), nosocomial infections, especially those due to multi-drug resistant (MDR) strains, are increasingly detected. This study reports the case of a 50-year-old man with blisters on the right knee for 8 months, first admitted through the outpatient department for incisional biopsy. Microbiological and histo-pathological examination confirmed the diagnosis of blisters extending deeply up to the knee joint caused by MDR- A. baumannii. A broad spectrum antibiotic therapy was administered and later readjusted according to the results of microbiological culture and biopsy report. Intensive hemodynamic support was required. An extensive surgical debridement was promptly performed and repeated until complete control of the infection with intravenous colistins. Blisters were excised; wounds were dressed daily with chlorhexidine dressings and polymyxine-impregnated dressing. Wounds were finally covered with split-thickness skin grafts. The infection was overcome 120 days after admission. The graft take was 40%. Postoperative rehabilitation was required because of the functional limitation of lower limb movements at the knee joint. Follow-up at 8 months showed no functional deficit and an acceptable aesthetic result. AB-MDR affecting soft tissues is a life-threatening disease, especially in patients with poor immunity and limited access to health facilities, whose clinical diagnosis may sometimes be challenging. Early recognition and treatment represent the most important factors influencing survival. PMID:26411144

  8. Kinetics and leaching behaviors of aluminum from pharmaceutical blisters in sodium hydroxide solution

    Institute of Scientific and Technical Information of China (English)

    王重庆; 王晖; 顾帼华; 符剑刚; 刘又年

    2015-01-01

    A hydrometallurgical process was developed for recycling pharmaceutical blisters. Leaching aluminum from pharmaceutical blisters using sodium hydroxide (NaOH) solutions was investigated with respect to leaching behaviors and kinetics. A L9(34) orthogonal design of experiments suggests that the most significant factor is NaOH concentration followed by temperature and leaching time. Factorial experiments demonstrate that the leaching rate of aluminum increases with increasing of the factors. The optimum conditions are temperature of 70 °C, leaching time of 20 min, NaOH concentration of 1.25 mol/L, liquid-to-solid mass ratio of 15:1 and agitation speed of 400 r/min. Under optimum conditions, the leaching rate is up to 100%, implying that aluminum and polyvinyl chloride (PVC) plastic in pharmaceutical blisters are separated completely. Kinetics of leaching aluminum is best described by the product layer diffusion control model, and the activation energy is calculated to be 19.26 kJ/mol.

  9. Mass spectrometry based data of the blister fluid proteome of paediatric burn patients.

    Science.gov (United States)

    Zang, Tuo; Broszczak, Daniel A; Cuttle, Leila; Broadbent, James A; Tanzer, Catherine; Parker, Tony J

    2016-09-01

    The data presented here are associated with the article "The blister fluid proteome of paediatric burns" (Zang et al., 2016) [1]. Burn injury is a highly traumatic event for children. The degree of burn severity (superficial-, deep-, or full-thickness injury) often dictates the extent of later scar formation which may require long term surgical operation or skin grafting. The data were obtained by fractionating paediatric burn blister fluid samples, which were pooled according to burn depth and then analysed using data dependent acquisition LC-MS/MS. The data includes a table of all proteins identified, in which burn depth category they were found, the percentage sequence coverage for each protein and the number of high confidence peptide identifications for each protein. Further Gene Ontology enrichment analysis shows the significantly over-represented biological processes, molecular functions, and cellular components of the burn blister fluid proteome. In addition, tables include the proteins associated with the biological processes of "wound healing" and "response to stress" as examples of highly relevant processes that occur in burn wounds. PMID:27536711

  10. Investigation of the Cause of Low Blister Threshold Temperatures in the RERTR-12 and AFIP-4 Experiments

    Energy Technology Data Exchange (ETDEWEB)

    Mitchell K Meyer

    2012-06-01

    Blister–threshold testing of fuel plates is a standard method through which the safety margin for operation of plate-type in research and test reactors is assessed. The blister-threshold temperature is indicative of the ability of fuel to operate at high temperatures for short periods of time (transient conditions) without failure. This method of testing was applied to the newly developed U-Mo monolithic fuel system. Blister annealing studies on the U-Mo monolithic fuel plates began in 2007, with the Reduced Enrichment for Research and Test Reactors (RERTR)-6 experiment, and they have continued as the U-Mo fuel system has evolved through the research and development process. Blister anneal threshold temperatures from early irradiation experiments (RERTR-6 through RERTR-10) ranged from 400 to 500°C. These temperatures were projected to be acceptable for NRC-licensed research reactors and the high-power Advanced Test Reactor (ATR) and the High Flux Isotope Reactor (HFIR) based on current safety-analysis reports (SARs). Initial blister testing results from the RERTR-12 experiment capsules X1 and X2 showed a decrease in the blister-threshold temperatures. Blister threshold temperatures from this experiment ranged from 300 to 400°C. Selected plates from the AFIP-4 experiment, which was fabricated using a process similar to that used to fabricate the RERTR-12 experiment, also underwent blister testing to determine whether results would be similar. The measured blister-threshold temperatures from the AFIP-4 plates fell within the same blister-threshold temperature range measured in the RERTR-12 plates. Investigation of the cause of this decrease in bister threshold temperature is being conducted under the guidance of Idaho National Laboratory PLN-4155, “Analysis of Low Blister Threshold Temperatures in the RERTR-12 and AFIP-4 Experiments,” and is driven by hypotheses. The main focus of the investigation is in the following areas: 1. Fabrication variables 2. Pre

  11. Intraoperative rupture of blood blister-like aneurysm: a case report and review of literature

    Institute of Scientific and Technical Information of China (English)

    Roodrajeetsing Gopaul; Wen Shao Xiao; Jun Yan; Dang Zong Wei

    2016-01-01

    Blood blister-like aneurysms (BBLAs) are aneurysms from the non-branching sites of the internal carotid artery (ICA).Though rare lesions,they pose a high risk of intraoperative aneurysmal rupture.Definite treatment of these types of aneurysms has been debatable,but surgical approach is the ultimate rescue treatment.Microsuture of the intraoperative ruptured BBLA has been reported scarcely in literature,but no review of these cases has ever been reported.We here present our experience of a case of BBLA intraoperative rupture requiring microsuture of the ICA and conduct meticulous review of all similar cases.

  12. Buckling patterns of gold thin films on silicon substrates: Formation of superimposed blisters

    Science.gov (United States)

    Colin, J.; Coupeau, C.; Durinck, J.; Grilhé, J.

    2009-06-01

    Buckling phenomena leading to the formation of superimposed blisters have been experimentally observed with the help of a confocal interferometric microscope onto the surface of gold thin films deposited on silicon substrates. Assuming that residual folding effects resulting from plastic deformation mechanisms take place in the film during its morphological evolution, different probable scenarios for the formation of the observed buckling patterns are elaborated in the framework of the Föppl-von Karman's theory of thin plates. Multi-step buckling with growing interface delamination is considered for the first scenario while a single or multi-step buckling at a given delamination width is assumed for the other ones.

  13. Management of rheumatic and autoimmune blistering disease in pregnancy and postpartum.

    Science.gov (United States)

    Wan, Joy; Imadojemu, Sotonye; Werth, Victoria P

    2016-01-01

    The treatment of rheumatic and autoimmune skin disease in women who are pregnant or of childbearing potential can present challenges to the dermatologist. We discuss the current approaches to treating lupus erythematosus, antiphospholipid antibody syndrome, dermatomyositis, morphea and systemic sclerosis, mixed connective tissue disease, rheumatoid arthritis, and autoimmune blistering disease in such patients. In the appropriate setting, topical and systemic corticosteroids, hydroxychloroquine, dapsone, azathioprine, and ultraviolet B phototherapy may be safely and cautiously used during pregnancy. Considerations about contraception, planned conception, therapeutic options, and disease control are paramount in optimizing pregnancy outcomes and minimizing risks to both mother and fetus. PMID:27265072

  14. Effect of inelastic and elastic energy losses of Xe ions on the evolution of hydrogen blisters in silicon

    Science.gov (United States)

    Reutov, V. F.; Dmitriev, S. N.; Sokhatskii, A. S.; Zaluzhnyi, A. G.

    2016-01-01

    We analyze the effect of irradiation by heavy ions on the formation of blisters on the silicon surface preliminarily ion-doped with hydrogen. An attempt is made at differentiating inelastic and elastic processes of interaction between ions and Si atoms using bombardment of the sample with high-energy charged particles through a bent absorbing filter by varying the radiation doses and the energy of bombarding Xe ions. It is found that irrespective of specific ionization energy losses of heavy ions, the blister formation is completely suppressed in the zone of the inelastic interaction during postradiation annealing. Conversely, stimulated development of hydrogen porosity takes place at the same time in the zone of elastic interaction, which is manifested in the form of blisters and flaking.

  15. Influence of post-annealing time on blistering evolution in Si implanted with high-fluence H ions

    International Nuclear Information System (INIS)

    The influence of post-annealing time on blistering characteristics induced by 5 x 1016 cm-2 ion-implanted H in Si was studied in terms of the formation and growth of blisters. Ion energies consisted of 40 and 100 keV. Post-annealing treatments were carried out using furnace annealing (FA) at 400 and 500 deg. C for a duration of 0.25-3 h in a nitrogen ambient. Raman scattering spectroscopy (RSS), optical microscopy (OM), atomic force microscopy (AFM), and secondary ion mass spectrometry (SIMS) were utilized to analyze the defect complex phases, the appearance of optically-detectable blisters and craters, the average depth of craters, and the hydrogen and oxygen depth profiles in the implanted layer, respectively. Furthermore, a characteristic time for the growth of optically-detectable blisters which was determined from the blister-covered fractions for various post-annealing times is proposed and used as a criterion to identify the effectiveness in the formation and growth of optically-detectable blisters. The results revealed that the characteristic time for the 400 deg. C-annealed specimens in the 40 keV implant is much shorter than it is in the 100 keV one. However, the characteristic time for the 500 deg. C-annealed specimens in the 40 keV implant is slightly longer than it is in the 100 keV implant. In addition, both the characteristic times for the 500 deg. C-annealed specimens are much shorter than those for the 400 deg. C ones. The above-mentioned phenomena hold true for craters

  16. Purification of antifungal protein against blister bark pathogen of Casuarina equisetifolia J. R. Forster et G. Forster

    OpenAIRE

    Ghosh, M.; Thangamani, D.; Thapliyal, M.; Yasodha, R.; Gurumurthi, K.

    2004-01-01

    Aprotein extract from the leaves of Andrographis paniculata (Acanthaceae) was found to inhibit the spore germination and hyphal extension of Trichosporium vesiculosum, the blister bark pathogen of Casuarina equisetifolia. The antifungal protein component was further purified from the crude extract and the molecular mass of the toxic protein was estimated to be 39.5 kDa.

  17. Study of surface blistering in GaN by hydrogen implantation at elevated temperatures

    International Nuclear Information System (INIS)

    We have investigated mechanisms of ion-cut in H2+-implanted GaN by analyzing microstructural features of H2+-implanted GaN at room temperature, 573 K and 723 K. Using optical microscopy and transmission electron microscopy, it was found that the in-plane compressive stress induced by the H-implantation was necessary for H-platelet nucleation and growth. The control of implantation temperature is crucial for creating sufficient in-plane compressive stress to induce surface blistering. - Highlights: • Mechanisms of ion-cut in H2+ implanted GaN were investigated. • The implantation-induced dislocation loops are correlated with implantation temperature. • The dislocation loops decide the direction of in-plane stress

  18. Activated charcoal and baking soda to reduce odor associated with extensive blistering disorders

    Directory of Open Access Journals (Sweden)

    Chakravarthi Arun

    2008-01-01

    Full Text Available Background: Skin disease leading to extensive blistering and loss of skin is associated with a characteristic smell. Odor can cause physiologic disturbances such as increase in heart rate and respiratory rate. It can also cause nausea and vomiting and is disturbing to bystanders. Aims: To test odor reducing capability of activated charcoal. Methods: In this blinded experimental study we used putrefied amniotic membrane to produce odor and studied the effectiveness of activated charcoal and soda-bi-carbonate to reduce odor. Results: Statistical analysis with Kruskal Wall′s Chi Square Test and Man Whitney U test showed significant reduction of odor using activated charcoal by itself or along with soda-bi-carbonate. Conclusion: We recommend the usage of activated charcoal with/without soda bicarbonate as an inexpensive practical measure to reduce foul odor associated with extensive skin loss.

  19. White blister rusts and downy mildews from bajaur agency fata, with some new records from pakistan

    International Nuclear Information System (INIS)

    In a species diversity study of Oomycyctes of Bajaur Agency FATA, Pakistan, infection of white blister rusts and downy mildews recorded on three cultivated and four wild plants. Capsella bursa-pastoris showed mixed infection of Albugo candida and Hyaloperonospora parasitica (syn: Peronospora parasitica). Similarly, A. candida and H. brassicae (syn: P. brassicae) parasitized Brassica campestris. Wilsoniana portulacae (syn: Albugo portulacae) and W. occidentalis com. nov. (syn: Albugo occidentalis) recovered from Portulaca oleracea and Spinacia oleracea, respectively. Bremia taraxaci, B. sonchicola and B. saussureae recorded on Taraxicum officinale, Sonchus sp., and Saussurea sp., respectively. All these obligate parasites are new records from Bajaur Agency, while H. parasitica, W. occidentalis, B. taraxaci, and B. saussureae on the mentioned hosts are new records from Pakistan. (author)

  20. Cytoskeletal Regulation of Inflammation and Its Impact on Skin Blistering Disease Epidermolysis Bullosa Acquisita.

    Science.gov (United States)

    Kopecki, Zlatko; Ludwig, Ralf J; Cowin, Allison J

    2016-01-01

    Actin remodelling proteins regulate cytoskeletal cell responses and are important in both innate and adaptive immunity. These responses play a major role in providing a fine balance in a cascade of biological events that results in either protective acute inflammation or chronic inflammation that leads to a host of diseases including autoimmune inflammation mediated epidermolysis bullosa acquisita (EBA). This review describes the role of the actin cytoskeleton and in particular the actin remodelling protein called Flightless I (Flii) in regulating cellular inflammatory responses and its subsequent effect on the autoimmune skin blistering disease EBA. It also outlines the potential of an antibody based therapy for decreasing Flii expression in vivo to ameliorate the symptoms associated with EBA. PMID:27420054

  1. Genetic transformation with the gfp gene of Colletotrichum gloeosporioides isolates from coffee with blister spot

    Directory of Open Access Journals (Sweden)

    Cecilia Armesto

    2012-09-01

    Full Text Available Blister spot (Colletotrichum gloeosporioides is now widespread in most coffee producing states of Brazil, becoming a limiting factor for production. The lack of data relating to the reproduction of typical symptoms (light green, oily patches leaves a gap within the pathosystem, forcing the search for new methodologies for monitoring the disease. Monitoring of genetically modified organisms has proven to be an effective tool in understanding the host x pathogen interactions. Thus, the present study was carried out to evaluate the effectiveness of two systems of genetic transformation in obtaining mutants using the gfp reporter gene. Using the two transformation systems (PEG and electroporation revealed the efficiency of both, confirmed by fluorescence microscopy and resistance to the antibiotic hygromycin-B, when incorporated into the culture medium. The fungus maintained its cultural and morphological characteristics when compared to wild strains. When inoculated on coffee seedlings, it was found that the pathogenicity of the processed isolates had not changed.

  2. Autoantibodies to Multiple Epitopes on the Non-Collagenous-1 Domain of Type VII Collagen Induce Blisters

    OpenAIRE

    Vorobyev, Artem; Ujiie, Hideyuki; Recke, Andreas; Buijsrogge, Jacqueline J. A.; Jonkman, Marcel F.; Pas, Hendrikus; Iwata, Hiroaki; HASHIMOTO, TAKASHI; Kim, Soo-Chan; Kim, Jong Hoon; Groves, Richard; Samavedam, Unni; Gupta, Yask; Schmidt, Enno; Zillikens, Detlef

    2015-01-01

    Epidermolysis bullosa acquisita (EBA) is an autoimmune blistering disease of the skin and mucous membranes, characterized by autoantibodies against type VII collagen (COL7), a major component of anchoring fibrils. Different clinical EBA phenotypes are described, including mechanobullous and inflammatory variants. Most EBA patients' sera react with epitopes located within the non-collagenous 1 (NC1) domain of human COL7. However, it has remained unclear whether antibody binding to these differ...

  3. ON THE PRESENCE OF CERTAIN BODIES IN THE SKIN AND BLISTER FLUID FROM SCARLET-FEVER AND MEASLES.

    Science.gov (United States)

    Field, C W

    1905-07-15

    In sections of control and normal skin, the nuclei of the epithelial cells were often indented by the cell protoplasm, giving them an appearance similar to those indented by Mallory's bodies. It would seem that if these bodies of Mallory's were protozoa they would have been found in the sections from both the living and the dead skin of scarlet-fever and measles, as they were present in the blister fluid. Their absence is certainly more suggestive of a degeneration than of a protozoon. This view is also borne out by the fact that they were not found immediately after death, but were present in another specimen from the same case removed twenty-four hours later. It would seem probable also that the bodies found in the blister fluid were the products of degeneration and cytolytic activity, because they were found in the antitoxin rashes as well as in the cases of scarlet-fever and measles. The histological changes in the skin of these two diseases leads us to expect the presence of cytolytic products both in the blister fluid and in the sections. It certainly cannot be stated that none of these bodies is a protozoön, but it can be positively stated that a great majority of them arise from degenerating cells; and in many cases, I think, it is not possible to differentiate a degeneration from a protozoön by the study of its morphology and staining reactions. The bodies present in blister fluid resemble very closely those granular bodies found in blood under certain conditions, and seen in vaccine lymph and in emulsions of tissues and in exudates. I think, therefore, that they are for the most part, if not wholly, products of degenerating tissue cells and of leucocytes, and within certain limits specific to scarlet-fever and measles.

  4. Metamodeling and Optimization of a Blister Copper Two-Stage Production Process

    Science.gov (United States)

    Jarosz, Piotr; Kusiak, Jan; Małecki, Stanisław; Morkisz, Paweł; Oprocha, Piotr; Pietrucha, Wojciech; Sztangret, Łukasz

    2016-06-01

    It is often difficult to estimate parameters for a two-stage production process of blister copper (containing 99.4 wt.% of Cu metal) as well as those for most industrial processes with high accuracy, which leads to problems related to process modeling and control. The first objective of this study was to model flash smelting and converting of Cu matte stages using three different techniques: artificial neural networks, support vector machines, and random forests, which utilized noisy technological data. Subsequently, more advanced models were applied to optimize the entire process (which was the second goal of this research). The obtained optimal solution was a Pareto-optimal one because the process consisted of two stages, making the optimization problem a multi-criteria one. A sequential optimization strategy was employed, which aimed for optimal control parameters consecutively for both stages. The obtained optimal output parameters for the first smelting stage were used as input parameters for the second converting stage. Finally, a search for another optimal set of control parameters for the second stage of a Kennecott-Outokumpu process was performed. The optimization process was modeled using a Monte-Carlo method, and both modeling parameters and computed optimal solutions are discussed.

  5. Degradation of the blister agent sulfur mustard, bis(2-chloroethyl) sulfide, on concrete.

    Science.gov (United States)

    Brevett, Carol A S; Sumpter, Kenneth B; Wagner, George W; Rice, Jeffrey S

    2007-02-01

    The products formed from the degradation of the blister agent sulfur mustard [bis(2-chloroethyl) sulfide] on concrete were identified using gas chromatography with mass spectrometry detection (GC/MSD), (1)H NMR, 2D (1)H-(13)C NMR and (13)C solid state magic angle spinning (SSMAS) NMR. In situ and extraction experiments were performed. Sulfur mustard was detected in the in situ (13)C SSMAS samples for 12 weeks, whereas less than 5% of the sulfur mustard was detected in extracts from the concrete monoliths after 8 days. Sulfonium ions and (2-chloroethylthio)ethyl ether (T) were observed on the in situ samples after a period of 12 weeks, whereas vinyl species and bis(2-chloroethyl) sulfoxide were observed in the extracts of the concrete monoliths within 24h. The differences between the extraction and the SSMAS data indicated that the sulfur mustard existed in the concrete in a non-extractable form prior to its degradation. Extraction methods alone were not sufficient to identify the products; methods to identify the presence of non-extractable degradation products were also required.

  6. Immunofluorescence patterns in selected dermatoses, including blistering skin diseases utilizing multiple fluorochromes

    Directory of Open Access Journals (Sweden)

    Ana Maria Abreu-Velez

    2015-01-01

    Full Text Available Background: Autoimmune vesiculobullous disorders represent a heterogeneous group of dermatoses whose diagnosis is made based on clinical history, histologic features, and immunopathologic features. The most commonly used techniques for the diagnosis of these diseases are direct and indirect immunofluorescence (DIF and IIF, including salt-split processing. NaCl split skin is used to determine the level of blister formation, and the localization of autoantibodies relative to the split. Classically, immunofluorescence has been performed with one fluorochrome in the diagnosis of autoimmune bullous skin diseases. Aims: To compare DIF and IIF of the skin, using a single fluorochrome versus multiple fluorochromes. Materials and Methods: We studied 20 autoimmune skin disease cases using fluorescein isothiocyanate (FITC alone, in comparison to multiple fluorochromes (with or without DNA counterstaining. Results: The use of multiple fluorochromes helped to simultaneously visualize reactivity in multiple skin areas, in contrast to using FITC alone. Conclusions: Using multiple fluorochromes allows simultaneous labeling of two or more antigens within the same cell/or tissue section, assists in colocalization of unknown antigens with known molecules, and helps in ruling out "background" staining.

  7. Characterization of Five Novel Mitoviruses in the White Pine Blister Rust Fungus Cronartium ribicola

    Science.gov (United States)

    Liu, Jun-Jun; Chan, Danelle; Xiang, Yu; Williams, Holly; Li, Xiao-Rui; Sniezko, Richard A.; Sturrock, Rona N.

    2016-01-01

    The white pine blister rust (WPBR) fungus Cronartium ribicola (J.C. Fisch.) is an exotic invasive forest pathogen causing severe stem canker disease of native white pine trees (subgenus Strobus) in North America. The present study reports discovery of five novel mitoviruses in C. ribicola by deep RNA sequencing. The complete genome of each mitovirus was determined by rapid amplification of cDNA ends (RACE) and reverse transcriptase-polymerase chain reaction (RT-PCR). A single open reading frame (ORF) encoding a putative RNA-dependent RNA polymerase (RdRp) was detected in each of the viral genomes using mitochondrial genetic codes. Phylogenetic analysis indicated that the C. ribicola mitoviruses (CrMV1 to CrMV5) are new putative species of the genus Mitovirus. qRT-PCR and RNA-Seq analyses revealed that viral RNAs were significantly increased in fungal mycelia in cankered pine stems compared to expression during two different stages of spore development, suggesting that viral genome replication and transcription benefit from active growth of the host fungus. CrMVs were widespread with relatively high levels of minor allele frequency (MAF) in western North America. As the first report of mitoviruses in the Class Pucciniomycetes, this work allows further investigation of the dynamics of a viral community in the WPBR pathosystem, including potential impacts that may affect pathogenicity and virulence of the host fungus. PMID:27196406

  8. Transcriptome Analysis Reveals Candidate Genes involved in Blister Blight defense in Tea (Camellia sinensis (L) Kuntze).

    Science.gov (United States)

    Jayaswall, Kuldip; Mahajan, Pallavi; Singh, Gagandeep; Parmar, Rajni; Seth, Romit; Raina, Aparnashree; Swarnkar, Mohit Kumar; Singh, Anil Kumar; Shankar, Ravi; Sharma, Ram Kumar

    2016-01-01

    To unravel the molecular mechanism of defense against blister blight (BB) disease caused by an obligate biotrophic fungus, Exobasidium vexans, transcriptome of BB interaction with resistance and susceptible tea genotypes was analysed through RNA-seq using Illumina GAIIx at four different stages during ~20-day disease cycle. Approximately 69 million high quality reads were assembled de novo, yielding 37,790 unique transcripts with more than 55% being functionally annotated. Differentially expressed, 149 defense related transcripts/genes, namely defense related enzymes, resistance genes, multidrug resistant transporters, transcription factors, retrotransposons, metacaspases and chaperons were observed in RG, suggesting their role in defending against BB. Being present in the major hub, putative master regulators among these candidates were identified from predetermined protein-protein interaction network of Arabidopsis thaliana. Further, confirmation of abundant expression of well-known RPM1, RPS2 and RPP13 in quantitative Real Time PCR indicates salicylic acid and jasmonic acid, possibly induce synthesis of antimicrobial compounds, required to overcome the virulence of E. vexans. Compendiously, the current study provides a comprehensive gene expression and insights into the molecular mechanism of tea defense against BB to serve as a resource for unravelling the possible regulatory mechanism of immunity against various biotic stresses in tea and other crops. PMID:27465480

  9. Characterization of Five Novel Mitoviruses in the White Pine Blister Rust Fungus Cronartium ribicola.

    Science.gov (United States)

    Liu, Jun-Jun; Chan, Danelle; Xiang, Yu; Williams, Holly; Li, Xiao-Rui; Sniezko, Richard A; Sturrock, Rona N

    2016-01-01

    The white pine blister rust (WPBR) fungus Cronartium ribicola (J.C. Fisch.) is an exotic invasive forest pathogen causing severe stem canker disease of native white pine trees (subgenus Strobus) in North America. The present study reports discovery of five novel mitoviruses in C. ribicola by deep RNA sequencing. The complete genome of each mitovirus was determined by rapid amplification of cDNA ends (RACE) and reverse transcriptase-polymerase chain reaction (RT-PCR). A single open reading frame (ORF) encoding a putative RNA-dependent RNA polymerase (RdRp) was detected in each of the viral genomes using mitochondrial genetic codes. Phylogenetic analysis indicated that the C. ribicola mitoviruses (CrMV1 to CrMV5) are new putative species of the genus Mitovirus. qRT-PCR and RNA-Seq analyses revealed that viral RNAs were significantly increased in fungal mycelia in cankered pine stems compared to expression during two different stages of spore development, suggesting that viral genome replication and transcription benefit from active growth of the host fungus. CrMVs were widespread with relatively high levels of minor allele frequency (MAF) in western North America. As the first report of mitoviruses in the Class Pucciniomycetes, this work allows further investigation of the dynamics of a viral community in the WPBR pathosystem, including potential impacts that may affect pathogenicity and virulence of the host fungus. PMID:27196406

  10. Transcriptome Analysis Reveals Candidate Genes involved in Blister Blight defense in Tea (Camellia sinensis (L) Kuntze)

    Science.gov (United States)

    Jayaswall, Kuldip; Mahajan, Pallavi; Singh, Gagandeep; Parmar, Rajni; Seth, Romit; Raina, Aparnashree; Swarnkar, Mohit Kumar; Singh, Anil Kumar; Shankar, Ravi; Sharma, Ram Kumar

    2016-07-01

    To unravel the molecular mechanism of defense against blister blight (BB) disease caused by an obligate biotrophic fungus, Exobasidium vexans, transcriptome of BB interaction with resistance and susceptible tea genotypes was analysed through RNA-seq using Illumina GAIIx at four different stages during ~20-day disease cycle. Approximately 69 million high quality reads were assembled de novo, yielding 37,790 unique transcripts with more than 55% being functionally annotated. Differentially expressed, 149 defense related transcripts/genes, namely defense related enzymes, resistance genes, multidrug resistant transporters, transcription factors, retrotransposons, metacaspases and chaperons were observed in RG, suggesting their role in defending against BB. Being present in the major hub, putative master regulators among these candidates were identified from predetermined protein-protein interaction network of Arabidopsis thaliana. Further, confirmation of abundant expression of well-known RPM1, RPS2 and RPP13 in quantitative Real Time PCR indicates salicylic acid and jasmonic acid, possibly induce synthesis of antimicrobial compounds, required to overcome the virulence of E. vexans. Compendiously, the current study provides a comprehensive gene expression and insights into the molecular mechanism of tea defense against BB to serve as a resource for unravelling the possible regulatory mechanism of immunity against various biotic stresses in tea and other crops.

  11. Molecular Mechanism Underlying Pathogenesis of Lewisite-Induced Cutaneous Blistering and Inflammation: Chemical Chaperones as Potential Novel Antidotes.

    Science.gov (United States)

    Li, Changzhao; Srivastava, Ritesh K; Weng, Zhiping; Croutch, Claire R; Agarwal, Anupam; Elmets, Craig A; Afaq, Farrukh; Athar, Mohammad

    2016-10-01

    Lewisite is a potent arsenic-based chemical warfare agent known to induce painful cutaneous inflammation and blistering. Only a few modestly effective antidotes have so far been described in the literature. However, the discovery of effective antidotes for lewisite was hampered by the paucity of the exact molecular mechanism underlying its cutaneous pathogenesis. We investigated the molecular mechanism underlying lewisite-induced cutaneous blistering and inflammation and describe its novel antidotes. On the basis of our initial screening, we used a highly sensitive murine model that recapitulates the known human pathogenesis of arsenicals-induced cutaneous inflammation and blistering. Topically administered lewisite induced potent acute inflammation and microvesication in the skin of Ptch1(+/-)/SKH-1 mice. Even at a very low dose, lewisite up-regulates unfolded protein response signaling, inflammatory response, and apoptosis. These cutaneous lesions were associated with production of reactive oxygen species and extensive apoptosis of the epidermal keratinocytes. We confirmed that activation of reactive oxygen species-dependent unfolded protein response signaling is the underlying molecular mechanism of skin damage. Similar alterations were noticed in lewisite-treated cultured human skin keratinocytes. We discovered that chemical chaperone 4-phenyl butyric acid and antioxidant N-acetylcysteine, which significantly attenuate lewisite-mediated skin injury, can serve as potent antidotes. These data reveal a novel molecular mechanism underlying the cutaneous pathogenesis of lewisite-induced lesions. We also identified novel potential therapeutic targets for lewisite-mediated cutaneous injury. PMID:27528504

  12. Blood blister-like aneurysms: Single center experience and systematic literature review

    Energy Technology Data Exchange (ETDEWEB)

    Gonzalez, Ana Marcos; Narata, Ana Paula; Yilmaz, Hasan [Interventional Neuroradiology Unit, Service of Neuroradiology, University Hospital of Geneva (Switzerland); Bijlenga, Philippe; Radovanovic, Ivan; Schaller, Karl [Service of Neurosurgery, University Hospital of Geneva (Switzerland); Lovblad, Karl-Olof [Interventional Neuroradiology Unit, Service of Neuroradiology, University Hospital of Geneva (Switzerland); Pereira, Vitor Mendes, E-mail: vitormpbr@hotmail.com [Interventional Neuroradiology Unit, Service of Neuroradiology, University Hospital of Geneva (Switzerland)

    2014-01-15

    Blood blister-like aneurysms (BBAs) are a controversial entity. They arise from non-branching sites on the supraclinoid internal carotid artery (ICA) and are suspected to originate from a dissection. Our aim is to describe the BBA cases seen in our center and to present a systematic review of the literature on BBAs. We analyzed the eleven cases of BBA admitted to our center from 2003 to 2012. We assessed the medical history, treatment modality (endovascular and/or surgery), complications and clinical outcome. The cohort included 8 women and 4 men with a mean age of 53.16 years. Treatment of the BBA consisted of stenting and coiling in 5 patients, stenting only in 4 patients, coiling and clipping in 1 patient, clipping only in 1 patient, and conservative treatment in 1 patient. A good outcome was found in 10 patients, as defined by a modified Rankin Scale (mRS) less than or equal to two at three months. A systematic review of the literature was performed, and 314 reported patients were found: 221 patients were treated with a primarily surgical approach, and 87 patients were treated with a primarily endovascular approach. A rescue or second treatment was required in 46 patients (21%). The overall estimated treatment morbidity rate was 17%, and the mortality rate was 15%. BBAs exhibit more aggressive behavior compared to saccular aneurysms, and more intra-operative complications occur with BBAs, independent of the treatment type offered. They are also significantly more likely to relapse and rebleed after treatment. Endovascular treatment offers a lower morbidity–mortality compared with surgical approaches. Multilayer flow-diverting stents appear to be a promising strategy.

  13. Detection of blister formation and evaluation of pressure tube/calandria tube contact location by ultrasonic velocity ratio measurement technique

    International Nuclear Information System (INIS)

    Presence of hydrogen in zircaloy pressure tube affects the velocity of ultrasound propagation. Both longitudinal wave velocity (VL) and shear wave velocity (VS) are affected depending on the concentration of hydrogen. Velocity ratio (VL/VS) changes as per the concentrations of hydrogen in different locations along the length of pressure tube. A hydride blister which forms at the pressure tube and calandria tube contact point is a distinct zone containing hydrogen 2-3 order of magnitude more than the parent matrix and hence, can be detected by sharp change in velocity ratio. (author)

  14. Blister pouches for effective reagent storage and release for low cost point-of-care diagnostic applications

    Science.gov (United States)

    Smith, Suzanne; Sewart, Rene; Land, Kevin; Roux, Pieter; Gärtner, Claudia; Becker, Holger

    2016-03-01

    Lab-on-a-chip devices are often applied to point-of-care diagnostic solutions as they are low-cost, compact, disposable, and require only small sample volumes. For such devices, various reagents are required for sample preparation and analysis and, for an integrated solution to be realized, on-chip reagent storage and automated introduction are required. This work describes the implementation and characterization of effective liquid reagent storage and release mechanisms utilizing blister pouches applied to various point-of-care diagnostic device applications. The manufacturing aspects as well as performance parameters are evaluated.

  15. The Effect of the Holding Time on Roll Bonded AA6061 Plates During The Blister Test In MTR-Type Fuel Plates Manufacturing

    International Nuclear Information System (INIS)

    Roll bonding process is applied to clad nuclear fuel core (U3O8+Al) with medium strength aluminum alloys during fabrication of plate type nuclear fuel elements for research reactors, or to produce new constructions which have special uses in industrial applications. In the present work, the effect of holding time at heating temperature during the blister test on the roll bonding of 6061 aluminum alloy plates was studied using both mechanical testing and microscopic examination. The present results showed that, the holding time during the blister test plays an important role in the criteria of bonding not only in the blister phenomena, but also in the bond strength. The specific bond strength increases with increasing the holding time. The results showed that the increase in the specific bond strength depends also on the degree of reduction in thickness during the rolling process. It varies from 4 % to 25 % for the cases of 40 % and 89 % reduction in thickness, respectively. On the other hand, It was found that more than 60 % of blistering area was formed during the first 15 minutes at 500 degree C. The remaining blistering area was formed during the next 30 minutes. (author)

  16. The viability of PVC/Al blister reuse and PVC property studies after ionizing radiation processing

    International Nuclear Information System (INIS)

    The objective of this research was to separate, by means of a process of dissolution, the PVC and the aluminum that compose blister packs, generally used for pharmaceutical pills. We also studied the effect of the ionizing radiation on the PVC, and, finally, the mechanical recycling of the separated PVC, by a process of extrusion. The material we used in this work is the surplus of the pharmaceutical industry, i.e., packs with defects or burrs. We ground the material to facilitate the handling and the homogenization of the system. After that, we chose two bases for the dissolution of the aluminum: the sodium hydroxide and the potassium hydroxide. We used a system with two concentrations (1 and 2M) for each base, and for every solution we had also an agitated and a non-agitated process. From this method resulted eight experiments. After the dissolution, the samples of the material were submitted to ionizing radiation with doses of 50, 100, 150 and 200 kGy in the Dynamitron II electron accelerator of the CTR-IPEN/CNEN-SP. In the following, these samples were submitted to traction resistance tests to analyze which modifications the irradiation caused. The last step of the research was the recycling of the PVC separated from the Aluminum. We made the recycling in industrial equipment, a PVC tube extruder. The material was combined with lubricants, heat stabilizers and pigment in an intensive mixer and processed into the form of rigid PVC electrical conduits. After the eight experiments, the system with potassium hydroxide base, concentration of 2M and agitation presented the best relation between time of dissolution and characteristics of the resulting material, without degradation of the PVC. In the irradiated samples, the color of the material changed as well as its extension that was as larger as the dose of irradiation they received, indicating the dissociation of the PVC molecules. The extrusion of the PVC was successfully realized: about 200 kg (440 pounds) of

  17. Influence of MeV H+ ion beam flux on cross-linking and blister formation in PMMA resist

    Directory of Open Access Journals (Sweden)

    Somrit Unai

    2012-02-01

    Full Text Available In soft lithography, a pattern is produced in poly(dimethylsiloxane (PDMS elastomer by casting from a master mould. The mould can be made of poly(methylmethacrylate (PMMA resist by utilising either its positive or negative tone induced by an ion beam. Here we have investigated the irradiation conditions for achieving complete cross-linking and absence of blister formation in PMMA so that its negative characteristic can be used in making master moulds. PMMA thin films approximately 9 µm thick on Si were deposited by spin coating. The 2-MeV H+ ion beam was generated using a 1.7-MV tandem Tandetron accelerator. The beam was collimated to a 500×500 µm2 cross section using programmable proximity aperture lithography system with a real-time ion beam monitoring system and a high precision current integrator. The irradiated areas were investigated by a standard scanning electron microscope and a profilometer. It was found that both the ion beam flux and the stopping power of the ions in the polymer have a critical influence on the blister formation.

  18. CYCLO-OXYGENASE 2 IS PRESENT IN THE MAJORITY OF LESIONAL SKIN FROM PATIENTS WITH AUTOINMUNE BLISTERING DISEASES

    Directory of Open Access Journals (Sweden)

    Ana Maria Abreu Velez

    2013-10-01

    Full Text Available Introduction: The in situ immune response within skin biopsies from patients affected by autoimmune skin blistering diseases (ABDs is not well characterized. Aim: Based on the fact that the ABD immune response is considered an adaptive immune response, both an innate immune response and inflammation would be expected in these diseases. Our investigation investigates the presence of cyclo-oxygenase-2 (COX-2, since this enzyme is commonly involved in innate immune responses. Methods: We utilized immunohistochemistry (IHC to evaluate the presence of COX-2 in lesional skin biopsies of patients affected by ABDs. We tested 30 patients with endemic pemphigus foliaceus (EPF, 15 controls from the endemic area, and 15 biopsies from healthy controls from the USA. We also tested archival biopsies from patients with selected ABDs, including 20 patients with bullous pemphigoid, 20 with pemphigus vulgaris, 8 with pemphigus foliaceus and 12 with dermatitis herpetiformis. Results: Most ABD biopsies stained positive for COX-2 in the lesional blister and/or the dermal inflammatory infiltrate, accentuated in the upper neurovascular plexus. In BP and EPF, the COX-2 staining was also seen in the sweat glands. All controls were negative. Conclusions: We document that COX-2 is expressed in lesional skin of patients with ABDs.

  19. Absorption of bacampicillin and ampicillin and penetration into body fluids (skin blister fluid, saliva, tears) in healthy volunteers.

    Science.gov (United States)

    Simon, C; Malerczyk, V; Klaus, M

    1978-01-01

    Equimolar doses of bacampicillin, which is rapidly converted to ampicillin in the body by hydrolysis, and ampicillin were administered orally, in the case of ampicillin also by intravenous injection, to 10 healthy subjects (cross-over study). Comparison of the areas under the serum concentrations curves after intravenous and oral administration showed that bacampillin was absorbed to 95% and orally given ampicillin to 35%. The mean peak serum levels (Cmax) after 0.8 g of oral bacampicillin were higher (15.9 microgram/ml) and appeared earlier (tmax 60 min) than after 0.556 g of oral ampicillin (3.2 microgram/ml, tmax 150 min). One and three hours after oral administration skin blister fluid contained four times more ampicillin after doses of bacampicillin than after oral ampicillin. One hour after intravenous injection of ampicillin the skin blister concentrations were 20 times higher than after oral administration of this antibiotic and three to four times higher than after oral administration of bacampicillin. The levels in saliva and tears were also determined and showed similar relationships. Since higher peaks serum levels resulted in higher and longer lasting concentrations in the extravascular space, bacampicillin is to be preferred for oral therapy.

  20. INFLUENCE OF PLANT DENSITIES AND PLANTING DATES ON THE POPULATION OF PIGEONPEA FLOWER BLISTER BEETLES IN OWERRI, IMO STATE, NIGERIA

    Directory of Open Access Journals (Sweden)

    SA DIALOKE

    2014-03-01

    Full Text Available The early maturing pigeonpea cultivar (ICPL 84023 was evaluated to ascertain the extent of protection to which plant densities and dates of planting will have on the population of blister beetles (Mylabris pustulata Thunberg (Coleoptera: Meloidae. Field trial was carried out at the Postgraduate Teaching and Research Farm, Department of Crop Science and Technology, Federal University of Technology, Owerri Imo State in April, July and October 2009 and repeated in 2010. Experiment was laid out in a 3 x 4 factorial and treatments consisted of four plant populations namely: 190,474 plants ha-1, 125,000 plants ha-1, and 80,000 plants ha-1, 55,556 plants ha-1 with three planting dates, April, July, and October. The result showed high population of M. pustulata on higher plant densities than at lower plant densities. Also April planting season recorded very high population of the M. pustulata on the pigeonpea while there was absence of the blister beetles during July and October planting seasons. Appropriate plant density and time of sowing may be an important integrated management of M. pustulata population in this locality.

  1. CD1a, HAM56, CD68 and S-100 are present in lesional skin biopsies from patients affected by autoimmune blistering diseases

    Directory of Open Access Journals (Sweden)

    Ana Maria Abreu Velez

    2014-04-01

    Full Text Available Introduction: Previous research on autoimmune skin blistering diseases (ABD has primarily focused on the humoral immune response; moreover, little attention has been given to the potential role of the antigen presenting cells (APCs in lesional skin. Aim: The purpose of our study was to immunophenotype selected APC in the lesional skin of ABDs, utilizing immunohistochemistry (IHC stains. Materials and Methods: We utilized IHC to stain for dendritic cells (DC, staining with CD1a, CD68, HAM56, and S-100 in lesional skin from 30 patients with endemic pemphigus foliaceus (EPF, 15 controls from the EPF endemic area, and 15 healthy controls from the USA. We also tested archival biopsies from patients with selected ABD, including 30 patients with bullous pemphigoid (BP, 20 with pemphigus vulgaris (PV, 8 with pemphigus foliaceus (PF and 14 with dermatitis herpetiformis (DH and 2 with epidermolysis bullosa acquisita (EBA. Results: Cells stained by CD68, HAM56 and S-100 were present in the majority of the ABD skin biopsies; these cells were located primarily in perivascular infiltrates surrounding dermal vessels subjacent to the blisters. However, these cells were also noted within the blisters, in vessels supplying dermal eccrine glands and ducts, and in areas of dermal endothelial-mesenchymal cell junction-like structures, especially in BP cases. In our CD1a staining, the number and location of positive staining cells varied with each disease, being abundant in most ABD in the epidermis suprajacent to the blisters, or in the epidermis surrounding the blister site if the blister site epidermis was missing. In the control biopsies, most did not display positive IHC staining, with the exception of a few CD1a positive cells in the epidermis Conclusion: Our findings confirm positive IHC staining for APCs in areas of the skin besides the disease blisters. Our findings suggest that the antigen presentation in ABD proceeds in areas distant from the blister site

  2. Influence Of Temperature On The Rate Of Copper Recovery From The Slag Of The Flash Direct-To-Blister Process By A Solid Carbon Reducer

    Directory of Open Access Journals (Sweden)

    Madej P.

    2015-09-01

    Full Text Available The aim of the work was to investigate the influence of temperature on the rate of copper removal from the obtained slag from the flash direct-to-blister process by means of a carbon reducer. The slag used in this work was taken from the direct-to-blister Outokumpu flash furnace at the smelter in Głogów, and graphite penetrators were used as the slag reducers. The experiment was carried out at 1573 K, 1623 K and 1673 K. It was found that the rate of the de-coppering process of the “Głogów” slag increased with the increase of temperature.

  3. Direct clipping of a blister-like aneurysm in the supraclinoid segment of the internal carotid artery: a clinical analysis of nine cases

    OpenAIRE

    Yu, Jinlu; Xu, Baofeng; Guo, Yunbao; Xu, Kan

    2015-01-01

    To investigate the method and outcome from direct clipping of a blister-like aneurysm (BBA) of the internal carotid artery supraclinoid segment. This study featured nine BBA patients, including eight females and one male. The patients presented subarachnoid hemorrhage (SAH) as the initial symptom and were diagnosed with BBA in the supraclinoid segment through a vascular exam and imaging. During the exam, the internal carotid artery was pressed to block the blood flow, and a transcranial Doppl...

  4. Endovascular internal carotid artery trapping for ruptured blood blister-like aneurysms: long-term results from a single centre

    Energy Technology Data Exchange (ETDEWEB)

    Kim, Byong-Cheol [Dongguk University Ilsan Hospital, Department of Neurosurgery, Goyang (Korea, Republic of); Kwon, O-Ki; Oh, Chang Wan; Bang, Jae Seung; Hwang, Gyojun [Seoul National University Bundang Hospital, Department of Neurosurgery, Seongnam, Gyeonggi (Korea, Republic of); Jin, Sung-Chul [Inje University Haeundae Paik Hospital, Department of Neurosurgery, Busan (Korea, Republic of); Park, Hyun [Gyeongsang National University Hospital, Department of Neurosurgery, Gyeongsangnam-do (Korea, Republic of)

    2014-03-15

    Endovascular internal carotid artery (ICA) trapping was performed to treat ruptured blood blister-like aneurysms (BBAs). The aim of this study was to evaluate the procedural risks and long-term follow-up results. The records of 11 consecutive patients with BBAs who underwent endovascular ICA trapping between 2005 and 2010 were reviewed. Clinical outcomes were assessed with modified Rankin Scale (mRS) scores. Endovascular ICA trapping was performed in 11 patients as either the primary treatment (7 patients) or the secondary treatment (4 patients) after the patient underwent other treatments. Three patients underwent superficial temporal artery (STA)-middle cerebral artery (MCA) bypass when balloon test occlusion (BTO) revealed inadequate collateral circulation. In the primary ICA trapping group (seven patients), six patients had good outcomes (mRS 0 in five, mRS 1 in one), and one patient had a poor outcome (mRS 6: dead). In the secondary ICA trapping group (four patients), two patients had good outcomes (mRS 0), and two patients had poor outcomes (mRS 4, 5). All ten of the surviving patients were clinically stable during the follow-up period (mean 39 months). A radiological follow-up of nine patients (mean 22 months) demonstrated stable occlusion, with the exception of one reopening of the ICA because of coil migration. Perfusion studies of nine patients (mean: 23 months) demonstrated no perfusion decrease. Endovascular ICA trapping is an effective and durable treatment for BBAs. (orig.)

  5. Endovascular internal carotid artery trapping for ruptured blood blister-like aneurysms: long-term results from a single centre

    International Nuclear Information System (INIS)

    Endovascular internal carotid artery (ICA) trapping was performed to treat ruptured blood blister-like aneurysms (BBAs). The aim of this study was to evaluate the procedural risks and long-term follow-up results. The records of 11 consecutive patients with BBAs who underwent endovascular ICA trapping between 2005 and 2010 were reviewed. Clinical outcomes were assessed with modified Rankin Scale (mRS) scores. Endovascular ICA trapping was performed in 11 patients as either the primary treatment (7 patients) or the secondary treatment (4 patients) after the patient underwent other treatments. Three patients underwent superficial temporal artery (STA)-middle cerebral artery (MCA) bypass when balloon test occlusion (BTO) revealed inadequate collateral circulation. In the primary ICA trapping group (seven patients), six patients had good outcomes (mRS 0 in five, mRS 1 in one), and one patient had a poor outcome (mRS 6: dead). In the secondary ICA trapping group (four patients), two patients had good outcomes (mRS 0), and two patients had poor outcomes (mRS 4, 5). All ten of the surviving patients were clinically stable during the follow-up period (mean 39 months). A radiological follow-up of nine patients (mean 22 months) demonstrated stable occlusion, with the exception of one reopening of the ICA because of coil migration. Perfusion studies of nine patients (mean: 23 months) demonstrated no perfusion decrease. Endovascular ICA trapping is an effective and durable treatment for BBAs. (orig.)

  6. Identification and Biochemical Characterization of Protein Phosphatase 5 from the Cantharidin-Producing Blister Beetle, Epicauta chinensis

    Directory of Open Access Journals (Sweden)

    Xi'en Chen

    2013-12-01

    Full Text Available Protein phosphatase 5 (PP5 is a unique member of serine/threonine phosphatases which has been recognized in regulation of diverse cellular processes. A cDNA fragment encoding PP5 (EcPP5 was cloned and characterized from the cantharidin-producing blister beetle, E. chinensis. EcPP5 contains an open reading frame of 1500 bp that encodes a protein of 56.89 kDa. The deduced amino acid sequence shares 88% and 68% identities to the PP5 of Tribolium castaneum and humans, respectively. Analysis of the primary sequence shows that EcPP5 has three TPR (tetratricopeptide repeat motifs at its N-terminal region and contains a highly conserved C-terminal catalytic domain. RT-PCR reveals that EcPP5 is expressed in all developmental stages and in different tissues. The recombinant EcPP5 (rEcPP5 was produced in Escherichia coli and purified to homogeneity. The purified protein exhibited phosphatase activity towards pNPP (p-nitrophenyl phosphate and phosphopeptides, and its activity can be enhanced by arachidonic acid. In vitro inhibition study revealed that protein phosphatase inhibitors, okadaic acid, cantharidin, norcantharidin and endothall, inhibited its activity. Further, protein phosphatase activity of total soluble protein extract from E. chinensis adults could be impeded by these inhibitors suggesting there might be some mechanism to protect this beetle from being damaged by its self-produced cantharidin.

  7. TISSUE INHIBITOR OF METALLOPROTEINASE 1, MATRIX METALLOPROTEINASE 9, ALPHA-1 ANTITRYPSIN, METALLOTHIONEIN AND UROKINASE TYPE PLASMINOGEN ACTIVATOR RECEPTOR IN SKIN BIOPSIES FROM PATIENTS AFFECTED BY AUTOIMMUNE BLISTERING DISEASES

    Directory of Open Access Journals (Sweden)

    Ana Maria Abreu Velez

    2013-07-01

    Full Text Available Introduction: Proteinases and proteinase inhibitors have been described to play a role in autoimmune skin blistering diseases. We studied skin lesional biopsies from patients affected by several autoimmune skin blistering diseases for proteinases and proteinase inhibitors. Methods: We utilized immunohistochemistry to evaluate biopsies for alpha-1-antitrypsin, human matrix metalloproteinase 9 (MMP9, human tissue inhibitor of metalloproteinases 1 (TIMP-1, metallothionein and urokinase type plasminogen activator receptor (uPAR. We tested 30 patients affected by endemic pemphigus, 30 controls from the endemic area, and 15 normal controls. We also tested 30 biopsies from patients with bullous pemphigoid (BP, 20 with pemphigus vulgaris (PV, 8 with pemphigus foliaceus, and 14 with dermatitis herpetiformis (DH. Results: Contrary to findings in the current literature, most autoimmune skin blistering disease biopsies were negative for uPAR and MMP9. Only some chronic patients with El Bagre-EPF were positive to MMP9 in the dermis, in proximity to telocytes. TIMP-1 and metallothionein were positive in half of the biopsies from BP patients at the basement membrane of the skin, within several skin appendices, in areas of dermal blood vessel inflammation and within dermal mesenchymal-epithelial cell junctions.

  8. Analysis of Blistering Mechanism for Dispersion-Type Fuel Plates During Irradiation%弥散型燃料板的辐照起泡机理分析

    Institute of Scientific and Technical Information of China (English)

    高利军; 陈炳德; 姜胜耀; 肖忠; 俞冀阳; 张林; 李垣明

    2012-01-01

    弥散型燃料在研究堆和动力堆中有着广泛的应用.起泡是弥散型燃料特有的失效模式,起泡的发生将导致堆芯传热性能恶化,威胁反应堆的运行安全.在分析总结国内外弥散型燃料板的辐照后起泡退火试验结果的基础上,从微观尺度到宏观尺度分析了起泡发生的机理,重点研究了弥散型燃料板的一种重要起泡模式——孔洞连通模式,剖析了孔洞连通发生的3个基本过程.同时应用孔洞连通机理,在估算裂变气体压力的前提下,通过力学计算给出了可引起起泡的孔洞连通的圆形区域尺度约为1.8 mm,这与实验观察结果相符.本文分析表明,燃料板的孔洞连通起泡机理涉及到高燃耗效应、燃料相的肿胀和开裂、裂变碎片损伤和应力腐蚀开裂等过程,建立起泡模型需做弹塑性力学和断裂力学的数值计算.%Dispersion fuels are widely used in research reactors and power reactors. Blistering is a special failure mode for dispersion fuels, which can reduce the efficiency of thermal removal in the core and threaten the nuclear reactor safety. Based on the results of blistering of dispersion-type fuel plates during post-irradiation annealing experiments worldwide, the mechanism of blistering was studied both at the microscopic scale and at the macroscopic scale and the important blistering mode known as void interconnection, which consisted of three separate processes, was emphasized and elaborated. Using the proposed mechanism and assuming the estimation of the pressure caused by fission gas, the dimension of the round region which could induce blistering was calculated using mechanics method and the value of 1. 8 mm was gained, showing good consistency with the experimental observation. This work shows that the proposed void interconnection mechanism of blistering is closely related to the high burnup structure, swelling and cracking of fuel phase, fission fragment damage and stress

  9. Blister Test for Measurements of Adhesion and Adhesion Degradation of Organic Polymers on AA2024-T3

    Science.gov (United States)

    Rincon Troconis, Brendy Carolina

    A key parameter for the performance of corrosion protective coatings applied to metals is adhesion. Surface preparation prior to coating application is known to be critical, but there is a lack of understanding of what controls adhesion. Numerous techniques have been developed in the last decades to measure the adhesion strength of coatings to metals. Nonetheless, they are generally non-quantitative, non-reproducible, performed in dry conditions, or overestimate adhesion. In this study, a quantitative and reproducible technique, the Blister Test (BT), is used. The BT offers the ability to study the effects of a range of parameters, including the presence or absence of a wetting liquid, and simulates the stress situation in the coating/substrate interface. The effects of roughness and surface topography were studied by the BT and Optical Profilometry, using AA2024-T3 substrates coated with polyvinyl butyral (PVB). Random abrasion generated a surface with lower average roughness than aligned abrasion due to the continual cross abrasion of the grooves. The BT could discern the effects of different mechanical treatments. An adhesion strength indicator was defined and found to be a useful parameter. The effectiveness of standard adhesion techniques such as ASTM D4541 (Pull-off Test) and ASTM D3359 (Tape Test) was compared to the BT. Also, different attempts to measure adhesion and adhesion degradation of organic polymers to AA2024-T3 were tested. The pull-off test does not produce adhesive failure across the entire interface, while the tape test is a very qualitative technique and does not discern between the effects of different coating systems on the adhesion performance. The BT produces adhesive failure of the primer studied, is very reproducible, and is able to rank different coating systems. Therefore, it was found to be superior to the others. The approaches tested for adhesion degradation were not aggressive enough to have a measurable effect. The effects of

  10. IN SITU IMMUNE RESPONSE EVALUATION VIA IMMUNOHISTOCHEMISTRY IN SKIN BIOPSIES FROM PATIENTS AFFECTED BY AUTOIMMUNE BLISTERING DISEASES

    Directory of Open Access Journals (Sweden)

    Ana Maria Abreu Velez

    2013-11-01

    Full Text Available Introduction: The in situ immune response in skin biopsies from patients affected by autoimmune skin blistering diseases (ABD is not well characterized. Aim: Our investigation attempts to immunophenotype cells in lesional skin in several ABD, utilizing immunohistochemistry (ICH. Methods: We tested by IHC for CD4, CD8, CD19, CD20, CD45, CD56/NCAM, PAX-5, granzyme B, myeloperoxidase, neutrophil elastase, LAT and ZAP-70 in patients affected by ABD. We tested 30 patients with endemic pemphigus foliaceus (EPF, 15 controls from the EPF endemic area, and 15 biopsies from healthy controls from the USA. We also tested archival biopsies from patients with selected ABD, including 30 patients with bullous pemphigoid, 20 with pemphigus vulgaris, 8 with pemphigus foliaceus and 14 with dermatitis herpetiformis. Results: We found a predominantly CD8 positive/CD45 positive T cell infiltrate in all ABD. Our skin biopsies demonstrated consistently positive staining for myeloperoxidase, but negative staining for neutrophil elastase. Most ABD biopsies displayed negative staining for CD4 and B cell markers; natural killer cell markers were also rarely seen. ZAP-70 and LAT were frequently detected. In El Bagre-EPF, a significant fragmentation of T cells in lesional skin was noted, as well as autoreactivity to lymph nodes. Conclusions: The documented T cell and myeloperoxidase staining are indicative of the role of T lymphocytes and neutrophils in lesional biopsies in patients with ABD, in addition to previously documented deposition of B cells, immunoglobulins and complement in situ. In El Bagre-EPF, T cells could also target lymph nodes; however, further studies are needed to confirm this possibility.

  11. A Novel Approach to the Quantitation of Coeluting Cantharidin and Deuterium Labelled Cantharidin in Blister Beetles (Coleop-tera: Meloidae

    Directory of Open Access Journals (Sweden)

    W Boland

    2007-11-01

    Full Text Available Blister beetles (Coleoptera: Meloidae are the main natural source of cantharidin, but the compound titre is depended on several factors including, age, sex and mating status of the insects. In order to eliminate such uncertainty factors in physio¬logical and chemical studies deuterium labelled cantharidin (D2C with no natural abundance is normally introduced into the beetles' body to use it as a model for studying the cantharidin behaviour in vivo. Experiments were achieved on Mylabris quadripunctata (Col.: Meloidae from Southern France and the beetles were exposed to an artificial diet containing a de¬fined amount of D2C. On the other hand, because of the high similarity between the two compounds they cannot be well quantified by gas chromatography. In order to remove the burden, MRM technique was used for the first time which could successfully create well-defined cantharidin and D2C peaks and hence a precise measurement. MRM technique was exam¬ined using a GC-MS Varian Saturn which collected MS/MS data of more than one compound in the same time window of the chromatogram. It is especially useful when coeluting compounds have different parent ions, i.e. m/z 84 for D2C (coelut¬ing isotopically-labelled compound and m/z 82 for cantharidin (beetle-originated compound. Using the routine GC-MS runs, measurement accuracy may be significantly reduced because the D2C peak is covered by the cantharidin huge peak while MRM could reveal the two coincided peaks of cantharidin and D2C. Therefore MRM is hereby introduced as the method of choice to separate cantharidin from D2C with high sensitivity and thus provide a precise base of quantitation.

  12. A Novel Approach to the Quantitation of Coeluting Cantharidin and Deuterium Labelled Cantharidin in Blister Beetles (Coleop-tera: Meloidae

    Directory of Open Access Journals (Sweden)

    MR Nikbakhtzadeh

    2007-12-01

    Full Text Available Blister beetles (Coleoptera: Meloidae are the main natural source of cantharidin, but the compound titre is depended on several factors including, age, sex and mating status of the insects. In order to eliminate such uncertainty factors in physio¬logical and chemical studies deuterium labelled cantharidin (D2C with no natural abundance is normally introduced into the beetles' body to use it as a model for studying the cantharidin behaviour in vivo. Experiments were achieved on Mylabris quadripunctata (Col.: Meloidae from Southern France and the beetles were exposed to an artificial diet containing a de¬fined amount of D2C. On the other hand, because of the high similarity between the two compounds they cannot be well quantified by gas chromatography. In order to remove the burden, MRM technique was used for the first time which could successfully create well-defined cantharidin and D2C peaks and hence a precise measurement. MRM technique was exam¬ined using a GC-MS Varian Saturn which collected MS/MS data of more than one compound in the same time window of the chromatogram. It is especially useful when coeluting compounds have different parent ions, i.e. m/z 84 for D2C (coelut¬ing isotopically-labelled compound and m/z 82 for cantharidin (beetle-originated compound. Using the routine GC-MS runs, measurement accuracy may be significantly reduced because the D2C peak is covered by the cantharidin huge peak while MRM could reveal the two coincided peaks of cantharidin and D2C. Therefore MRM is hereby introduced as the method of choice to separate cantharidin from D2C with high sensitivity and thus provide a precise base of quantitation.

  13. Composição, proteólise, capacidade de derretimento e formação de "blisters" do queijo mussarela obtido pelos métodos tradicional e de ultrafiltração: composition, proteolysis, melting capacity and blisters formation Mozzarella by ultrafiltration

    Directory of Open Access Journals (Sweden)

    Patrícia D. Pizaia

    2003-12-01

    Full Text Available O objetivo deste trabalho foi comparar a composição, a proteólise, a capacidade de derretimento e a formação de "blisters" (bolhas em queijos tipo Mussarela fabricados com retentado de leite (MR de fator de concentração volumétrica (FCV de 2,34:1, com um queijo Mussarela padrão (MP fabricado com leite não ultrafiltrado. Foi realizado um ensaio de produção com 3 lotes de MR e um lote de MP. Determinou-se a composição do leite, retentado, soro, água de filagem e queijos e a proteólise, a capacidade de derretimento e a formação de "blisters" nos queijos com 7, 15, 30 e 60 dias de armazenamento refrigerado. MRs apresentaram maiores valores de pH e de porcentagem de cinzas e de proteína total e menores porcentagens de acidez titulável, gordura, gordura no extrato seco e sal quando comparadas a MP. Durante o tempo de estocagem, as MRs apresentaram menor proteólise e capacidade de derretimento, em todas as datas analisadas. A porcentagem de área coberta por 'blisters" na pizza e o diâmetro médio dos mesmos foram maiores na MP durante o primeiro mês de estocagem e depois ambos os tipos de queijos apresentaram comportamentos similares para estes 2 parâmetros.The objective of this research was to compare the composition, proteolysis, melting capacity and blisters formation in Mozzarella cheese manufactured with milk retentate (MR of a volumetric concentration factor (FCV of 2.34:1, with a standard Mozzarella cheese (MP manufactured with non ultrafiltrated milk. It was realized one production assay with 3 batches of MRs and one of MP. It was evaluated the milk, retentate, whey, stretching water and cheeses composition and the proteolysis, melting capacity and the blisters formation on cheeses with 7, 15, 30 and 60 days of refrigerates storage. MRs presented larger pH, ash and total protein contents and lower titratable acidity and fat, fat on dry matter and salt contents when compared to MP. Along the storage time the MRs

  14. High-separation efficiency micro-fabricated multi-capillary gas chromatographic columns for simulants of the nerve agents and blister agents

    OpenAIRE

    Li, Yi; Du, Xiaosong; Wang, Yang; Tai, Huiling; Qiu, Dong; Lin, Qinghao; Jiang, Yadong

    2014-01-01

    To achieve both high speed and separation efficiency in the separation of a mixture of nerve and blister agent simulants, a high-aspect-ratio micro-fabricated multi-capillary column (MCC, a 50-cm-long, 450-μm-deep, and 60-μm-wide four-capillary column) was fabricated by the application of the microelectromechanical system (MEMS) techniques. Mixtures of chemical warfare agent (CWA) simulants - dimethyl methylphosphonate (DMMP), triethyl phosphate (TEP), and methyl salicylate - were used as sam...

  15. Influence of the ionization-energy losses of high-energy bismuth ions on the development of helium blisters in silicon

    International Nuclear Information System (INIS)

    Understanding the behavior of helium in solids under conditions of intense ionizing radiation is of particular interest in solving many problems of nuclear, fusion, and space materials science and also in microelectronics. The observed effect of suppressing the formation of helium blisters on the surface of helium ion-doped silicon as a result of irradiation with high-energy bismuth ions is reported in this publication. It is suggested that a possible decrease in the concentration of helium atoms in silicon is due to their radiationinduced desorption from the area of doping in terms of the high-impact ionization of bismuth ions

  16. Blisters, Calluses, and Corns

    Science.gov (United States)

    ... their hands, which take a lot of abuse. Guitar players also get calluses — on their fingers — from ... to swing around the bars or play the guitar. Calluses on the feet, however, can be painful ...

  17. Increased endothelin-1 and diminished nitric oxide levels in blister fluids of patients with intermediate cold type complex regional pain syndrome type 1

    Directory of Open Access Journals (Sweden)

    Niehof Sjoerd

    2006-11-01

    Full Text Available Abstract Background In complex regional pain syndrome type 1 (CRPS1 pro-inflammatory mediators and vascular changes play an important role in the sustained development and outcome of the disease. The aim of this study was to determine the involvement of vasoactive substances endothelin-1 (ET-1 and nitric oxide (NO during early chronic CRPS1. Methods Included were 29 patients with CRPS 1 who were diagnosed during the acute stage of their disease and observed during follow-up visits. Disease activity and impairment were determined and artificial suction blisters were made on the CRPS1 and the contralateral extremities for measurements of IL-6, TNF-α, ET-1 and nitrate/nitrite (NOx. Results The levels of IL-6, TNF-α and ET-1 in blister fluid in the CRPS1 extremity versus the contralateral extremity were significantly increased and correlated with each other, whereas NOx levels were decreased. Conclusion The NOx/ET-1 ratio appears to be disturbed in the intermediate stage of CRPS, resulting in vasoconstriction and consequently in a diminished tissue blood distribution.

  18. Coma blisters after poisoning caused by central nervous system depressants: case report including histopathological findings Bolhas do coma pós-intoxicação por depressores do sistema nervoso central: relato de caso incluindo achados histopatológicos

    OpenAIRE

    Maira Migliari Branco; Eduardo Mello De Capitani; Maria Letícia Cintra; Stephen Hyslop; Adriana Camargo Carvalho; Fabio Bucaretchi

    2012-01-01

    Blister formation and eccrine sweat gland necrosis is a cutaneous manifestation associated with states of impaired consciousness, most frequently reported after overdoses of central nervous system depressants, particularly phenobarbital. The case of a 45-year-old woman who developed "coma blisters" at six distinct anatomic sites after confirmed (laboratory) phenobarbital poisoning, associated with other central nervous system depressants (clonazepam, promethazine, oxcarbazepine and quetiapine...

  19. Study on the Blistering of B4C/Al Neutron Absorber Plate in the Course of Corrosion%B4C/Al中子吸收板腐蚀过程中的起泡研究

    Institute of Scientific and Technical Information of China (English)

    李刚; 王美玲; 王贯春; 简敏

    2012-01-01

    The corrosion model of cladding blistering on B4C/A1 neutron absorber plate was established by Henry's law, and by means of the model to investigate the relationship between pore radius and temperature factors for blistering. Influence of pore size for the neutron absorber plate blistering was analyzed by using the pore capillary effects. The results show that the hydrogen pressure produced in small radius pores is higher due to Al corrosion, which contributed to B4C/A1 neutron absorber plate cladding blistering. Influence of temperature on the corrosion generated hydrogen pressure is very small, but the main factors affecting the hydrogen pressure is the pore radius sizes. Blistering power of the cladding is hydrogen continuous generation and accumulating in the pore capillary. The water diffuses easily through the pore capillary in B4C/A1 cores, which will cause the cladding blistering because of Al corrosion.%根据亨利定律,建立了B4C/Al中子吸收板包壳腐蚀起泡的模型,计算了孔隙半径、温度对起泡的影响,并利用孔隙毛细管效应分析了孔隙对中子吸收板起泡的影响.结果表明:孔隙半径越小,腐蚀产生的氢气压力越大,B4C/Al中子吸收板包壳更易起泡;温度对腐蚀产生的氢气压力影响非常小,而孔隙半径是影响氢气压力的主要因素;氢气持续产生并在孔隙毛细管中积聚,是包壳产生起泡的动力;水分容易通过孔隙毛细管在B4C/Al芯体中扩散,可导致Al腐蚀,引起包壳起泡.

  20. Effect of the order of He+ and H+ ion co-implantation on damage generation and thermal evolution of complexes, platelets, and blisters in silicon

    Science.gov (United States)

    Daghbouj, N.; Cherkashin, N.; Darras, F.-X.; Paillard, V.; Fnaiech, M.; Claverie, A.

    2016-04-01

    Hydrogen and helium co-implantation is nowadays used to efficiently transfer thin Si layers and fabricate silicon on insulator wafers for the microelectronic industry. The synergy between the two implants which is reflected through the dramatic reduction of the total fluence needed to fracture silicon has been reported to be strongly influenced by the implantation order. Contradictory conclusions on the mechanisms involved in the formation and thermal evolution of defects and complexes have been drawn. In this work, we have experimentally studied in detail the characteristics of Si samples co-implanted with He and H, comparing the defects which are formed following each implantation and after annealing. We show that the second implant always ballistically destroys the stable defects and complexes formed after the first implant and that the redistribution of these point defects among new complexes drives the final difference observed in the samples after annealing. When H is implanted first, He precipitates in the form of nano-bubbles and agglomerates within H-related platelets and nano-cracks. When He is implanted first, the whole He fluence is ultimately used to pressurize H-related platelets which quickly evolve into micro-cracks and surface blisters. We provide detailed scenarios describing the atomic mechanisms involved during and after co-implantation and annealing which well-explain our results and the reasons for the apparent contradictions reported at the state of the art.

  1. Targeted proteolysis of plectin isoform 1a accounts for hemidesmosome dysfunction in mice mimicking the dominant skin blistering disease EBS-Ogna.

    Directory of Open Access Journals (Sweden)

    Gernot Walko

    2011-12-01

    Full Text Available Autosomal recessive mutations in the cytolinker protein plectin account for the multisystem disorders epidermolysis bullosa simplex (EBS associated with muscular dystrophy (EBS-MD, pyloric atresia (EBS-PA, and congenital myasthenia (EBS-CMS. In contrast, a dominant missense mutation leads to the disease EBS-Ogna, manifesting exclusively as skin fragility. We have exploited this trait to study the molecular basis of hemidesmosome failure in EBS-Ogna and to reveal the contribution of plectin to hemidesmosome homeostasis. We generated EBS-Ogna knock-in mice mimicking the human phenotype and show that blistering reflects insufficient protein levels of the hemidesmosome-associated plectin isoform 1a. We found that plectin 1a, in contrast to plectin 1c, the major isoform expressed in epidermal keratinocytes, is proteolytically degraded, supporting the notion that degradation of hemidesmosome-anchored plectin is spatially controlled. Using recombinant proteins, we show that the mutation renders plectin's 190-nm-long coiled-coil rod domain more vulnerable to cleavage by calpains and other proteases activated in the epidermis but not in skeletal muscle. Accordingly, treatment of cultured EBS-Ogna keratinocytes as well as of EBS-Ogna mouse skin with calpain inhibitors resulted in increased plectin 1a protein expression levels. Moreover, we report that plectin's rod domain forms dimeric structures that can further associate laterally into remarkably stable (paracrystalline polymers. We propose focal self-association of plectin molecules as a novel mechanism contributing to hemidesmosome homeostasis and stabilization.

  2. Morphology of a new blister beetle (Coleoptera, Meloidae larval type challenges the evolutionary trends of phoresy-related characters in the genus Meloe

    Directory of Open Access Journals (Sweden)

    A. Di Giulio

    2014-10-01

    Full Text Available The discovery of some specimens of a new first instar larval type in blister beetles, collected in Iran on Anthophora bees, confirms the existence of repetitive and parallel trends in morphological specialization to phoresy in distinct lineages of Meloidae and in particular in the subfamily Meloinae. The new Iranian larva, herein described and illustrated, shows several characters and a peculiar phoretic strategy that closely parallel that of the Meloe subgenus Lampromeloe, with similar modifications of the fronto-clypeal setae into strong lanceolate spines used to pierce the intersegmental membranes of the bees. Both parallel and shared derived evolution of these characters seem possible. The coexistence in this larva of characters in both primitive and derived state is of particular interest in order to analyse the different rates and trends of evolution of phoretic adaptations. A morphological comparison (SEM of this new meloine larva (incertae sedis, tentatively assignable to Meloe, with the M. (Lampromeloe larvae is carried out in order to discuss the evolutionary implications of its placement in Lampromeloe, and the relative characters that would support it, vs other possible alternative scenarios.

  3. Composição, proteólise, capacidade de derretimento e formação de "blisters" do queijo mussarela obtido pelos métodos tradicional e de ultrafiltração: composition, proteolysis, melting capacity and blisters formation Mozzarella by ultrafiltration

    OpenAIRE

    Patrícia D. Pizaia; Leila M. Spadoti; Agnes Narimatsu; José Raimundo F. Dornellas; Salvador M. Roig

    2003-01-01

    O objetivo deste trabalho foi comparar a composição, a proteólise, a capacidade de derretimento e a formação de "blisters" (bolhas) em queijos tipo Mussarela fabricados com retentado de leite (MR) de fator de concentração volumétrica (FCV) de 2,34:1, com um queijo Mussarela padrão (MP) fabricado com leite não ultrafiltrado. Foi realizado um ensaio de produção com 3 lotes de MR e um lote de MP. Determinou-se a composição do leite, retentado, soro, água de filagem e queijos e a proteólise, a ca...

  4. 增生性瘢痕水泡液β转化生长因子的变化及意义%TGF-β change in blister fluid of hypertrophic scar and its significance

    Institute of Scientific and Technical Information of China (English)

    谢有富; 陈辉; 黎庆梅; 梁达荣

    2010-01-01

    Objective To investigate of the tissue TGF-β changes at early stage of hypertrophic scar formation and the value of scar blisters in hypertrophic scar.Methods The TGF-β1 content in the blister fluid and the blood were quantified with ELISA,patients(n=15)with hypertrophy scar after depth burn were included,three time point(each n=5)on early stage(0.01),the TGF-β1 in the blister of normal skin was also not elevated(P>0.01),but TGF-β1 level in the scarblisters hypertrophic scar was elevated significantly[90 d,(534.4±125.9)pg/L,P0.01);增生性瘢痕水泡液TGF-β1含量显著高于其血液和正常皮肤水泡液(P均<0.01),而且瘢痕增生早期(1~3个月)组织TGF-β1逐渐升高(P<0.01).结论 瘢痕形成早期组织TGF-β含量增高可能是瘢痕增生的重要因素;瘢痕水泡液可作为研究增生性瘢痕的新途径.

  5. 颈内动脉血泡样动脉瘤的CTA诊断%CT angiography in the diagnosis of internal carotid artery blister-like aneuryms

    Institute of Scientific and Technical Information of China (English)

    陈状; 李林; 崔静; 公方和; 王伟民

    2012-01-01

    Objective To explore the utility ofCT angiography (CTA) in the identification and characterization of internal carotid artery (ICA) blood blister-like aneuryms (BBA). Methods All the 143 patients with spontaneous subarachnoid hemorrhage (SAH) who had been managed in our department from January 2008 to December 2009 were analyzed retrospectively.All of them underwent CTA prior to digital subtraction angiography (DSA) for evaluation of spontaneous SAH.The reports from the CTA and DSA were reviewed to determine whether the ICA BBA had been correctly and prospectively diagnosed. A retrospective review of the CTA and DSA images was also performed.Results All patients were diagnosed with spontaneous SAH by plain scan CT.We identified 6 cases of ICA BBA on initial DSA imaging.Of the 6 blister-like aneurysms,4 (67%) were identified prospectively and 5 (86%) retrospectively on CTA.In one case that had been confirmed by DSA as ICA BBA,the retrospective CTA failed to find the abnormity.All the 6 patients underwent endovascular treatment with stent placement.All patients were followed up for an average of 8.3 months (range,6-18 months) simply by DSA after treatment. Conclusion If CTA identifies a spontaneous SAH but fails to identify the cause,a careful DSA should be performed to detect a possible presence of ICA BBA.%目的 分析颈内动脉血泡样动脉瘤(BBA)CT三维血管重建(CTA)的诊断价值及形态特征. 方法 回顾性分析广州军区广州总医院神经外科自2008年1月至2009年12月收治的143例自发性蛛网膜下腔出血(SAH)患者资料.所有患者均先后行CTA及DSA检查,并对结果进行对比分析. 结果 所有患者经平扫CT诊断为自发性SAH,其中6例经DSA确诊为颈内动脉BBA.该6例患者中4例在DSA检查之前即经CTA确诊,未经CTA确诊的2例患者中1例后虽经DSA检查明确,但回顾分析CTA仍未发现明确病变.全部6例患者均经血管内支架治疗,术后均单

  6. Colonization history, host distribution, anthropogenic influence and landscape features shape populations of white pine blister rust, an invasive alien tree pathogen.

    Directory of Open Access Journals (Sweden)

    Simren Brar

    Full Text Available White pine blister rust is caused by the fungal pathogen Cronartium ribicola J.C. Fisch (Basidiomycota, Pucciniales. This invasive alien pathogen was introduced into North America at the beginning of the 20th century on pine seedlings imported from Europe and has caused serious economic and ecological impacts. In this study, we applied a population and landscape genetics approach to understand the patterns of introduction and colonization as well as population structure and migration of C. ribicola. We characterized 1,292 samples of C. ribicola from 66 geographic locations in North America using single nucleotide polymorphisms (SNPs and evaluated the effect of landscape features, host distribution, and colonization history on the structure of these pathogen populations. We identified eastern and western genetic populations in North America that are strongly differentiated. Genetic diversity is two to five times higher in eastern populations than in western ones, which can be explained by the repeated accidental introductions of the pathogen into northeastern North America compared with a single documented introduction into western North America. These distinct genetic populations are maintained by a barrier to gene flow that corresponds to a region where host connectivity is interrupted. Furthermore, additional cryptic spatial differentiation was identified in western populations. This differentiation corresponds to landscape features, such as mountain ranges, and also to host connectivity. We also detected genetic differentiation between the pathogen populations in natural stands and plantations, an indication that anthropogenic movement of this pathogen still takes place. These results highlight the importance of monitoring this invasive alien tree pathogen to prevent admixture of eastern and western populations where different pathogen races occur.

  7. Keratinocyte-targeted expression of human laminin γ2 rescues skin blistering and early lethality of laminin γ2 deficient mice.

    Directory of Open Access Journals (Sweden)

    Tracy L Adair-Kirk

    Full Text Available Laminin-332 is a heterotrimeric basement membrane component comprised of the α3, ß3, and γ2 laminin chains. Laminin-332 modulates epithelial cell processes, such as adhesion, migration, and differentiation and is prominent in many embryonic and adult tissues. In skin, laminin-332 is secreted by keratinocytes and is a key component of hemidesmosomes connecting the keratinocytes to the underlying dermis. In mice, lack of expression of any of the three Laminin-332 chains result in impaired anchorage and detachment of the epidermis, similar to that seen in human junctional epidermolysis bullosa, and death occurs within a few days after birth. To bypass the early lethality of laminin-332 deficiency caused by the knockout of the mouse laminin γ2 chain, we expressed a dox-controllable human laminin γ2 transgene under a keratinocyte-specific promoter on the laminin γ2 (Lamc2 knockout background. These mice appear similar to their wild-type littermates, do not develop skin blisters, are fertile, and survive >1.5 years. Immunofluorescence analyses of the skin showed that human laminin γ2 colocalized with mouse laminin α3 and ß3 in the basement membrane zone underlying the epidermis. Furthermore, the presence of "humanized" laminin-332 in the epidermal basement membrane zone rescued the alterations in the deposition of hemidesmosomal components, such as plectin, collagen type XVII/BP180, and integrin α6 and ß4 chains, seen in conventional Lamc2 knockout mice, leading to restored formation of hemidesmosomes. These mice will be a valuable tool for studies of organs deficient in laminin-332 and the role of laminin-332 in skin, including wound healing.

  8. Direct clipping of a blister-like aneurysm in the supraclinoid segment of the internal carotid artery: a clinical analysis of nine cases.

    Science.gov (United States)

    Yu, Jinlu; Xu, Baofeng; Guo, Yunbao; Xu, Kan

    2015-01-01

    To investigate the method and outcome from direct clipping of a blister-like aneurysm (BBA) of the internal carotid artery supraclinoid segment. This study featured nine BBA patients, including eight females and one male. The patients presented subarachnoid hemorrhage (SAH) as the initial symptom and were diagnosed with BBA in the supraclinoid segment through a vascular exam and imaging. During the exam, the internal carotid artery was pressed to block the blood flow, and a transcranial Doppler (TCD) was performed to examine the blood flow in the (BBA) ipsilateral middle cerebral artery. The results showed that the blood flow was reduced by less than 40% in all nine patients. During surgery, a pterional craniotomy was used to fully expose the aneurysm for clipping. While clipping the aneurysm, the arterial wall at the root of the aneurysm was also clipped to improve stability. Intraoperative ultrasonography was performed before and after clipping to examine the blood flow in the parent artery, middle cerebral artery and anterior cerebral artery. Moreover, a TCD was performed within one week after surgery to monitor intracranial blood flow. During BBA clipping, four patients exhibited stenosis of the parent artery, but intraoperative ultrasonography showed adequate blood flow in the anterior cerebral artery and middle cerebral artery. TCD was performed within one week after surgery to examine the blood flow in the middle cerebral artery on the operative side; the TCD showed adequate blood flow. The patients recovered well after surgery and were discharged. Digital subtraction angiography (DSA) or computed tomography angiography (CTA) was performed six to 12 months after the surgery and showed no aneurysm relapse. Moreover, six patients exhibited no stenosis of the parent artery, 20% stenosis was observed in one patient, 30% stenosis was observed in one patient, and 50% stenosis was observed in one patient; none of the patients showed clinical symptoms, and all

  9. Mechanism Analysis of Hydrogen Blisters on the Surface of a Gas Tank and Research on Its Preventive Measures%低分气分液罐表面氢鼓泡机理分析及预防措施研究

    Institute of Scientific and Technical Information of China (English)

    陈虎; 冯亚娟; 谈平庆; 王和慧; 侯峰

    2015-01-01

    Serious blisters were found on the shell surface of the gas tank which operated in wet H2S environment during a regular inspection. It was confirmed with gas sampling device that the gas in the blister was hydrogen. The causes of blisters were analyzed and investigated by chemical analysis, metallographic examines, scanning electron microscopy (SEM) and energy dispersive spectroscopy (EDS). The results revealed that the elongated MnS inclusions were the important reason for the hydrogen blisters. The preventive measures of the hydrogen blisters were put forward based on the above study.%某石化公司脱硫装置低分气分液罐在湿硫化氢环境中运行,检修时发现罐体外表面出现严重的鼓泡现象。本文利用气体取样装置测定鼓泡内气体为氢气,并通过宏观检查、金相组织分析、扫描电镜分析和能谱分析等测试方法对低分气分液罐氢鼓泡原因进行分析和研究,结果表明,长条形MnS夹杂物是导致罐体表面产生氢鼓泡的重要原因。在此基础上,提出氢鼓泡预防措施。

  10. Simultaneous determination of gold and silver in blister copper by inductively coupled plasma atomic emission spectrometry%电感耦合等离子体发射光谱法同时测定粗铜中金和银

    Institute of Scientific and Technical Information of China (English)

    刘益锋; 冯俊汉; 李展江; 史亚晓

    2012-01-01

    Blister copper sample were dissolved in mixed acid of hydrochloric acid and nitric acid. Gold and silver content was determined directly by inductively coupled plasma optical emission spectrometry (ICPOES) by using 242. 794 nm and 328. 068 nm as analyzing spectral line. Investigation of the effects of matrix and co-existing ions were studied. It was shown that copper had certain effect on the determination, but can be removed by matrix-matching, while other impurity elements had no influence. The method has been applied to the blister copper and the results are consistent with the results by the method of YS/T521. 2-2009, with the relative standard deviations of gold and silver (RSD, n = 12) smaller than 1. 9 % and 1.1% respectively. In addition, the recoveries of testing elements were between 95 % and 105 %.%粗铜样品用盐酸和硝酸混合酸溶解,选择242.794 nm和328.068 nm的光谱线分别作为金和银的分析线,直接用电感耦合等离子体发射光谱法(ICP-AES)同时测定了粗铜中金和银含量.考察了基体效应和共存离子等因素对测定的影响.实验表明,铜存在基体效应对测定有影响,但可以采用基体匹配的方法消除,粗铜中的其他杂质元素对测定没有影响.用本法测定了粗铜中的金和银含量,测定值与行业标准方法(YS/T521.2- 2009)的测定值相符,金、银测定结果的相对标准偏差分别小于1.9%和1.1%(n=12),加标回收率在95%~105%之间.

  11. Stress analysis of local blisters coupling Raman spectroscopy and X-ray diffraction. Correlation between experimental results and continuous damage modelling for buckling in an iron oxide/phosphated iron system

    Energy Technology Data Exchange (ETDEWEB)

    Panicaud, B., E-mail: benoit.panicaud@utt.fr [Universite de Technologie de Troyes (UTT), CNRS UMR 6279, 12 rue Marie Curie, 10010 Troyes (France); Grosseau-Poussard, J.L. [LEMMA, Pole Sciences et Technologie, Universite de La Rochelle, Av. M. Crepeau, 17042 La Rochelle Cedex (France)

    2010-12-01

    In this present work, local stress development in the iron oxide layers growing on phosphated {alpha}-Fe at 400 deg. C in ambient air is investigated by Raman spectroscopy. Coupled with X-ray diffraction it enables to obtain directly local stresses' maps in the oxide layers. Use of Raman spectroscopy allows obtaining better accuracy on mechanical behaviour at local scale. This characterisation technique is very useful to study systems developing mechanical heterogeneities on surface, especially in case of buckling phenomenon. Investigations on particular local blisters have been done to measure some characteristic lengths at local scale. From local measurements, we are able to evaluate general effect of buckling from simplified scale transition. So, a macroscopic approach has been performed to calculate global stress evolution of the oxide layer, based on continuous damage mechanics. Consequently, it leads to good comparison between modelling and experimental values (global stresses versus oxidation time) in {alpha}-Fe{sub 2}O{sub 3} oxide.

  12. Aspectos morfológicos da colonização de Colletotrichum gloeosporioides em órgãos de plantas de cafeeiros e com sintomas da mancha manteigosa Morphologic aspects of colonization of Colletotrichum gloeosporioides on organs of coffee plants with blister spot

    Directory of Open Access Journals (Sweden)

    Josimar Batista Ferreira

    2009-08-01

    Full Text Available Objetivou-se, no presente trabalho, analisar microscopicamente a colonização de C. gloeosporioides, agente da mancha manteigosa (MM, em condições naturais da doença, sobre os diferentes órgãos e tecidos da cultivar Catucaí Vermelho: folhas, pecíolos, nervuras, ramos, frutos e pedúnculos. Todas as amostras foram processadas, obtendo-se imagens em microscópico eletrônico de varredura. Os ramos e as nervuras de folhas de cafeeiros com mancha manteigosa apresentando morte descendente e hipocótilos oriundos de sementes têm os vasos do xilema, floema e células do córtex colonizados por C. gloeosporioides, já os frutos com sintoma da mancha manteigosa apresentaram colonização nos tecidos do exocarpo, mesocarpo, endocarpo e endosperma.The aim of this work was to study the colonization of leaves, petioles, veins, branches, fruits, and peduncles by C. gloeosporioides, the causal agent of blister spot, under natural infections of cultivar Catucaí Vermelho in field conditions. All materials were analyzed through scanning electron microscopy. The branches and veins of coffee leaves affected by blister spot with descending death as well as hypocotyls from seedlings had their xylem, phloem and cortical cells colonized by C. gloeosporioides while fruits showing blister spot symptoms had their exocarp, mesocarp, endocarp, and endosperm tissues colonized by C. gloeosporioides.

  13. Experimental Study of Changes of Skin Blister Fluid NPY, IL-12, sICAM-1 and GM-CSF Levels in Patients with Vitiligo in Progressive Stage%白癜风进展期患者皮肤疱液中NPY、sICAM-1、IL-12和GM-CSF水平变化的实验研究

    Institute of Scientific and Technical Information of China (English)

    毕鸣晔; 黄海峰

    2011-01-01

    目的:探讨白癜风病患者的发病、进展与免疫学机制的关系.方法:将确诊为进展期白癜风的80例患者分为寻常型(54例),节段型(26例)组.各以白斑处和非白斑处2组的疱液中的研究指标水平进行比较.疱液NPY和GM-CSF采用RIA;IL-12、sICAM-1水平均采用ELISA.结果:本文测定的患者疱液NPY水平显示,寻常型白癜风患者白斑处与非白斑处无显著差异(P>0.05),节段型白癜风患者白斑处与非白斑处比较则呈显著性差异(P0.05).结论:白癜风病患者的发病及病情进展与疱液NPY、IL-12、sICAM-1和GM-CSF四项指标的变化关系密切,其测定有助于了解其病因及病理学机制.%Objective To explore the significance of changes of skin blister fluid NPY, IL-12, sICAM-1 and GM-CSF levels in patients with vitiligo in progressive stage. Methods 80 patients with vitiligo in progressive stage were divided into two groups (vulgar-is vitiligo groups; n = 54, segmental vitiligo groups: n = 26) Their blister fluid levels of NPY and GM-CSF were determined by radioim-munoassay (RIA ) , and IL-12 and sICAM-1 were determined by enzyme immunoassay . Results The levels of skin blister fluid NPY were definitely higher in vitilignous skin than those in non- vitilignous patches in segmental vitiligo groups (P 0. 05) . The levels of skin blister fluid IL-12,sICAM-1 and GM-CSF were all obviously higher in vitilignous skin than that in non- vitilignous patches in vulgaris vitiligo groups (P 0. 05) . Conclusion The changes of skin blister fluid NPY, IL-12, sICAM-1 and GM-CSF levels in vitilignous skin may be closely related to development of difference type vitiligo patients with vitiligo, determination of 4 indexes might be helpful for studying the pathogenesis and clinical diagnosis of vitiligo.

  14. Coma blisters after poisoning caused by central nervous system depressants: case report including histopathological findings Bolhas do coma pós-intoxicação por depressores do sistema nervoso central: relato de caso incluindo achados histopatológicos

    Directory of Open Access Journals (Sweden)

    Maira Migliari Branco

    2012-08-01

    Full Text Available Blister formation and eccrine sweat gland necrosis is a cutaneous manifestation associated with states of impaired consciousness, most frequently reported after overdoses of central nervous system depressants, particularly phenobarbital. The case of a 45-year-old woman who developed "coma blisters" at six distinct anatomic sites after confirmed (laboratory phenobarbital poisoning, associated with other central nervous system depressants (clonazepam, promethazine, oxcarbazepine and quetiapine, is presented. A biopsy from the left thumb blister taken on day 4 revealed focal necrosis of the epidermis and necrosis of sweat gland epithelial cells; direct immunofluorescence was strongly positive for IgG in superficial blood vessel walls but negative for IgM, IgA, C3 and C1q. The patient was discharged on day 21 with no sequelae.Formação de bolhas e necrose de glândula sudoríparas écrinas é uma manifestação cutânea associada com estados de diminuição da consciência, mais frequentemente relatada após superdosagens de depressores do sistema nervoso central, particularmente fenobabital. Relatamos o caso de uma paciente de 45 anos que desenvolveu "bolhas do coma" após tentativa de suicídio por fenobarbital (confirmada laboratorialmente, associada a outros depressores do sistema nervoso central (clonazepam, prometazina, oxcarbazepina e quetiapina. Biópsia da bolha do 1o quirodáctilo esquerdo no 4o dia de internação revelou necrose focal da epiderme e necrose de células epiteliais de glândula sudorípara; a imunofluorescência direta foi fortemente positiva para IgG na parede superficial dos vasos sanguíneos, mas negativa para IgM, IgA, C3 e C1q. A paciente teve alta no 21o dia, sem seqüelas.

  15. 华山松疱锈病的重寄生真菌(深绿木霉)中几丁质酶基因cDNA片段的克隆%Cloning of cDNA Fragment of Chitinase Gene from the Mycoparasite Trichoderma atroviride on Armandii Pine Blister Rust

    Institute of Scientific and Technical Information of China (English)

    马长乐; 李靖; 陈玉惠; 刘小烛

    2008-01-01

    [Objective] The aim of this study was to isolate chitinase gene from Trichoderma atroviride strain SS003. [Method] With the aeciospore wall of armandii pine blister rust as inducer, chitinase gene was induced to express in Trichoderma atroviride cells. The cDNA fragment of chitinase gene was cloned by RT-PCR approach. [Result] The activity of chitinase induced reached 40.17 μg/10 min; and the specific fragment amplified was 834 bp in length and proved to be the fragment of chitinase gene by sequencing and sequence analysis. [Conclusion] The result showed the feasibility of isolating the full length of chitinase gene and its transformation, and further producing chitinase.

  16. 穴位贴敷与电针治疗支气管哮喘疗效对照观察%Controlled observation of non-blister acupoint sticking and electroacupuncture for bronchial asthma

    Institute of Scientific and Technical Information of China (English)

    田海燕; 胡佳; 王琳

    2013-01-01

    Objective To compare the differences of clinical efficacy for bronchial asthma between acupoint sticking combined with ginger-separated moxibustion and electroacupuncture combined with ginger-separated moxibustion.Methods With a randomized and controlled method,one hundred cases were divided into an acupoint sticking + ginger-separated moxibustion group (group A) and an electroacupuncture + ginger-separated moxibustion group (group E),50 cases in each one.The Dazhui (GV 14),Feishu (BL 13),Tiantu (CV 22) and so on were selected in both groups.The treatment was given on the 1st day of each dog day period and each nine-day period after the winter solstice separately.The ginger-separated moxibustion was applied in the group A,which was followed by plaster sticking that was made with Chinese herbs (including white mustard seed,rhizoma corydalis,euphorbia kansui,asarum),glycerin and warm ginger juice.The regular acupuncture,electroacupuncture and ginger-separated moxibustion were applied in the group E.The scores of recovery cough,control asthma,respiratory frequency,stuffiness chest,wheezing and markedly and cured effective rate were compared in two groups before and after the treatment.Results The markedly and cured effective rate was 72.0% (36/50) in the group A,which was superior to 46.0%(23/50) in the group E (P<0.05).All the symptom scores in two groups were obviously improved after the treatment,in which group A was superior to group E (all P<0.05).Conclusion The acupoint sticking and ginger separated moxibustion could obviously improve clinical symptoms in patients with bronchial asthma,and reduce skin irritation of medicine to avoid the blister and relive patients' suffer,which has superior effect to electroacupuncture combined with ginger-separated moxibustion.%目的:比较穴位贴敷加隔姜灸与电针加隔姜灸治疗支气管哮喘的临床疗效差异.方法:采用随机、对照的方法,将100例患者分为穴位贴敷加隔姜灸组(穴位

  17. 金银花洗液治疗口服索拉菲尼并发手足水疱的效果及护理%Nursing and effect of honeysuckle lotion on the patients taking sorafenib with the complication of hand and foot blisters

    Institute of Scientific and Technical Information of China (English)

    陈美丽; 温桂芬; 冯湘萍; 刘艳

    2012-01-01

    目的 探讨金银花洗液治疗原发性肝癌患者服用索拉菲尼后并发手足水疱的效果,并给予相应的护理.方法 选取口服索拉菲尼后发生手足水疱患者45例为研究对象,按随机数字表法将其分为实验组23例和对照组22例,对照组患者采用尿素软膏外涂,实验组患者在此基础上,给予金银花洗液浸泡.比较两组患者疗效及愈合时间.结果 治疗后实验组治疗有效率为91.3%,优于对照组的59.1%,差异有统计学意义(x2=8.494,P<0.05);实验组创面愈合时间为(8.5±4.2)d,优于对照组的(11.8±6.3)d,差异有统计学意义(t=2.92,P<0.05).结论 金银花洗液对促进原发性肝癌患者服用索拉菲尼后并发手足水疱愈合有明显效果,且缩短愈合时间.%Objective To explore the effect of honeysuckle lotion on the primary hepatic cancer patients taking sorafenib with the complication of hand and foot blisters.Methods Totals of 45 patients who took sorafenib and complicated with the hand and foot blisters were randomly divided into experimental group ( n =23 ) and control group( n =22).The experimental group were soaked in honeysuckle lotion accompany with topical urea ointment,while control group received topical urea ointment.Then,the clinical effective and healing time of two groups were observed.Results There was no significant difference detected in age,lesion and general between two groups.The clinical treatment effective rate of experimental group was 91.3% significantly better than 59.1% that of control group ( x2 =8.494,P < 0.05 ).The healing time of experimental group was (8.5 ± 4.2)d and that of control group was (11.8 ± 6.3 )d,and the difference was statistically significant (t =2.92,P < 0.05 ).Conclusions The honeysuckle lotion application can promote wound healing for primary hepatic cancer patients taking sorafenib with the complication of hand and foot blisters.And it is worthy for clinical application.

  18. Analysis of clinical efifcacy of famciclovir combined with genital herpes blister lfuid auto-inoculation in the treatment of genital herpes%泛昔洛韦联合生殖器疱疹疱液自体接种治疗生殖器疱疹的临床疗效分析

    Institute of Scientific and Technical Information of China (English)

    温斌

    2014-01-01

    目的:探讨泛昔洛韦联合生殖器疱疹疱液自体接种治疗生殖器疱疹的临床疗效。方法:将我院收治的86例生殖器疱疹患者随机分为试验组和对照组,对试验组患者给予泛昔洛韦联合生殖器疱疹疱液自体接种治疗,对对照组患者仅给予泛昔洛韦治疗,比较两治疗组患者的临床疗效、至临床症状改善的时间和不良反应的发生情况。结果:试验组患者的治愈率为83.72%、总有效率为100.00%,均显著高于对照组(分别为39.53%和93.02%,P均<0.05);试验组患者的复发率为16.28%,显著低于对照组的60.47%(P<0.05);试验组患者的至皮损愈合平均时间、自觉疼痛和瘙痒平均持续时间也均显著短于对照组(P均<0.05)。试验组中有2例患者发生轻度的乏力和发热,但这些不良反应均在2d内自行消失;对照组中未见明显不良反应发生。结论:泛昔洛韦联合生殖器疱疹疱液自体接种治疗能够显著提高生殖器疱疹的治疗效果、降低复发率,具有临床应用价值。%Objective: To explore the clinical efficacy of the autologous vaccination of genital herpes blister fluid in the treatment of genital herpes. Methods: Eighty-six cases of patients with genital herpes were randomly divided into an experimental group and a control group, in which famciclovir combined with the autologous vaccination of genital herpes blister lfuid were given in the experimental group while only famciclovir in the control group. Clinical efifcacy, time for the appearance of the improvement of clinical symptoms and incidence of adverse reaction were compared between two groups. Results:The rates of the cure and the total effectiveness were 83.72%and 100%in the experimental group, which were signiifcantly higher than those in the control group (39.53%and 93.02%, P<0.05);the recurrence rate was 16.28%in the experiment group, which was signiifcantly lower than that in the

  19. Effect of Acupoint Application with Wu's Blistering Plaster Combined with Seretide Inhalation in Preventing and Treating Children with Asthma:40 Cases Report%吴氏发泡膏穴位贴敷联合舒利迭吸入防治儿童哮喘40例临床观察

    Institute of Scientific and Technical Information of China (English)

    施品英; 朱金凤; 尤菊松; 沈鸣

    2011-01-01

    Objective To observe the effect of acupoint application with Wu's Blistering Plaster combined with seretide inhalation in preventing and treating children with asthma in remission stage and to study its influence on immune function. Methods Eighty cases of children with asthma in remission stage were randomized into two groups, 40 in treatment group and 40 in control group. Both groups were treated with seretide inhalation 50μg/100μg, one or two times a day for 6 months, based on which, the treatment group was treated with Wu's Blistering Plaster for acupoint application in dog-days. The effect and changes in symptom scores of both groups were observed, and serum IgE, IL-4, and IFN-γ were determined before and after treatment.Results The total effective rate of the treatment group and control group was 95.0% and 80. 0% respectively, with significant difference (P<0. 05). The treatment group was superior to the control group in increase of IFN-γ and decrease of total IgE and IL-4 (P<0. 05 or P<0. 01). Conclusion Acupoint application with Wu's Blistering Plaster combined with seretide inhalation is effective in improving the immune function of asthma children and its good at preventing and treating asthma.%目的 观察吴氏发泡膏穴位贴敷联合舒利迭吸人防治缓解期儿童哮喘的临床疗效及其对免疫功能的影响.方法 将80例哮喘缓解期患儿随机分成治疗组和对照组各40例,两组均正规吸入舒利迭50μg/100μg,视病情每日1~2次,共6个月,治疗组在此基础上于三伏期给予吴氏发泡膏穴位贴敷,观察两组患儿临床疗效、治疗前后症状评分变化及血清总免疫球蛋白E(IgE)、白细胞介素4(IL-4)、干扰素γ(IFN-γ)等指标的变化.结果 治疗组总有效率95.0%,对照组为80.0%,临床疗效治疗组优于对照组(P<0.05).治疗组IFN-γ升高水平及总IgE、IL-4、症状评分下降水平优于对照组(P<0.05或P<0.01).结论 吴氏发泡膏穴位贴

  20. 广东省地方标准《企鹅珍珠贝附壳珠培育技术规范》分析%Analysis on Guangdong Standard of Technical Specifications for Blister Pearl Production with Pteria penguin(RoeHng)

    Institute of Scientific and Technical Information of China (English)

    童银洪; 邓陈茂

    2012-01-01

    阐述了《企鹅珍珠贝附壳珠培育技术规范》广东省地方标准的编制目的和意义,介绍了《企鹅珍珠贝附壳珠培育技术规范(DB44/T741—2010)》的立项背景和起草工作过程、编制原则和适用范围,解读了《企鹅珍珠贝附壳珠培育技术规范(DB44/T741-2010)》的主要条款。%The compilation objective and significance of Guangdong local standard DB44/T 741-2010,technical specifications for blister pearl production with Pteria penguin ( Roeding )' were described. The background, drafting process, compilation principles and scope of application were introduced,the key chapters and terms were interpreted.

  1. 细纹豆芫菁3-羟甲基戊二酰辅酶A-还原酶基因全长cDNA克隆及生物信息学分析%Cloning and bioinformatical analysis of 3-hydroxy-3-methylglutaryl coenzyme A reductase gene from the blister beetle Epicauta mannerheimi ( Coleoptera: Meloidae)

    Institute of Scientific and Technical Information of China (English)

    姜鸣; 霍棠; 吕淑敏; 张雅林

    2012-01-01

    3-Hydroxy-3-methylglutaryl coenzyme A reductase ( HMGR) is a key enzyme of the mevalonate pathway. Obtaining HMGR gene information is the basis of studying the relationship between cantharidin biosynthesis and the mevalonate pathway. In this study, a full-length cDNA of the HMGR gene was cloned from the blister beetle Epicauta mannerheimi ( Maklin) by RACE technology, which was named EmHMGR (GeneBank accession no. JQ690539). The full-length of EmHMGR is 3 118 bp with an ORF of 2 526 bp, containing a 5'-untranslated region (5'-UTR) of 178 bp and a 3'UTR of 414 bp. It encodes a deduced protein of 842-amino acid residues, with a predicted molecular mass of 92.8 kDa and isoeletric point (pI) of 6.0. Its predicted molecular formula is C4135H6604N1098O1216S50. Bioinformatical analysis showed that its instability index is 43. 37 and the GRAVY 0.091, suggesting that EmHMGR is an unstable hydrophobic protein. The deduced protein EmHMGR has the conserved functional domain of HMGR_Class I and the sterol-sensing domain, and shares more than 50% amino acid identity with HMGRs from other insects. Phylogenetic analysis showed that EmHMGR has the closest relationship with HMGRs of chrysomelids. This study provides the basis for further research on the biosynthetic pathway of cantharidin in the blister beetles.%3-羟甲基戊二酰辅酶A-还原酶(3-hydroxy-3-methylglutaryl coenzyme Areductase,HMGR)是甲羟戊酸途径的关键酶.获得芫菁体内HMGR基因信息是确定甲羟戊酸途径与斑蝥素合成相关性的基础.本研究利用RACE技术从细纹豆芫菁Epicauta mannerheimi(M(a)klin)体内克隆获得HMGR基因全长cDNA序列,命名为EmHMGR(GenBank登录号为JQ690539).该基因全长3118 bp,其中5’端非翻译区178 bp,3’端非翻译区414 bp,开放阅读框2 526 bp,编码842个氨基酸.推测的蛋白质分子量为92.8 kDa,理论等电点为6.0,预测分子式为C4135H6604N1098O1216S50,不稳定系数为43.37,总亲水性系数为0.091,为疏水性不

  2. Hand, foot and scrotal blisters in a patient with cancer receiving oral chemotherapy

    OpenAIRE

    Guerra, Jose Rodolfo; Suelves, Ana M; Bella, Archie; Lolo, Delatre

    2014-01-01

    Tyrosine kinase inhibitors are novel antineoplastic drugs that make use of the molecular abnormalities that have been discovered in certain types of tumours. These agents are associated with important dermatological side effects. This case report discusses an atypical presentation of the hand–foot syndrome in one patient treated with sorafenib. A 78-year-old man, recently started on sorafenib for the treatment of hepatocarcinoma with lung metastasis, presented to the emergency room with painf...

  3. Neutron reflectivity of supported membranes incorporating terminally anchored polymers: Protrusions vs. blisters

    DEFF Research Database (Denmark)

    Fragneto, Giovanna; Halperin, Avraham; Klösgen-Buchkremer, Beate Maria;

    2013-01-01

    The effect of terminally anchored chains on the structure of lipid bilayers adsorbed at the solid/water interface was characterized by neutron reflectivity. In the studied system, the inner leaflet, closer to the substrate, consisted of head-deuterated 1,2-distearoyl-sn-glycero-3-phosphorylcholin...

  4. Blistering in alloy Ti–6Al–4V from H+ ion implantation

    Indian Academy of Sciences (India)

    B K Singh; V Singh

    2010-04-01

    The effect of H+ ion implantation on surface morphology of the titanium alloy, Ti–6Al–4V, was studied, following H+ ion implantation of 150 keV and 250 keV energy to fluence of 2.6 × 1018 cm-2 and 2.5 × 1019 cm-2, respectively at ambient temperature. No detectable change was observed in surface features of either of the above specimen immediately after the implantation. However, vein like features (VLF) were observed to appear on the surface of the sample, implanted at 150 keV to a fluence of 2.6 × 1018 cm-2, following natural ageing at room temperature for 150 days. Subsequent annealing of the above naturally aged sample, at 423 K for 150 min under vacuum (10-3 torr), led to development of a macroblister. In sharp contrast in the other sample, implanted by H+ ions of higher energy (250 keV) to higher fluence of 2.5 × 1019 cm-2, neither there was any effect of natural ageing following the implantation nor that of subsequent annealing at 423 K and ageing on its surface morphology.

  5. Analysis Of Separation Mechanism Of The Metallic Phase Of Slag In The Direct-To-Blister Process

    Directory of Open Access Journals (Sweden)

    Bydałek A.W.

    2015-09-01

    Full Text Available The article discusses the structure of the slag in the liquid state, the properties and interactions within the slag. The analysis of structures occurring in slag suspension were presented with regard to differences in chemical composition in micro-areas. Two different mechanisms for formation of precipitates in Cu-Fe-Pb alloys during extraction were showed.

  6. Influence of sulphur content and inclusion distribution on the hydrogen induced blister cracking in pressure vessel and pipeline steels

    Energy Technology Data Exchange (ETDEWEB)

    Domizzi, G.; Anteri, G.; Ovejero-Garcia, J. [Comision de Energia Atomica, Beunos Aires (Argentina). Depto. Materiales Gerencia Desarollo

    2001-07-01

    In previous works, extensive desulphurisation, including shape control and low hardness in segregation zones, were pointed out as effective ways for increasing resistance to hydrogen induced cracking (HIC) in steels exposed to wet H{sub 2}S environment. In this study, the behaviour of eight plates was evaluated by immersing samples in H{sub 2}S-saturated NACE solution. HIC susceptibility was assessed by an ultrasonic method and correlated with the elongated MnS inclusion distribution. The results allow us to remark that an extremely low sulphur level is not necessary to reach good HIC resistance, provided that no hard bands are present in the steel. Under this condition, a relationship to calculate the minimum average sulphide length which provides low HIC susceptibility was found. (author)

  7. Localized and generalized forms of blistering in junctional epidermolysis bullosa due to COL17A1 mutations in the Netherlands

    NARCIS (Netherlands)

    Pasmooij, A. M. G.; Pas, H. H.; Jansen, G. H. L.; Lemmink, H. H.; Jonkman, M. F.

    2007-01-01

    Background Mutations in the gene COL17A1 coding for type XVII collagen cause non-Herlitz junctional epidermolysis bullosa (nH-JEB). Objectives Here we give an overview of the genotype-phenotype correlation in 12 patients from the Netherlands with type XVII collagen-deficient nH-JEB. Patient and meth

  8. Triglyceride Blisters in Lipid Bilayers: Implications for Lipid Droplet Biogenesis and the Mobile Lipid Signal in Cancer Cell Membranes

    DEFF Research Database (Denmark)

    Khandelia, Himanshu; Duelund, Lars; Pakkanen, Kirsi Inkeri;

    2010-01-01

    Triglycerides have a limited solubility, around 3%, in phosphatidylcholine lipid bilayers. Using millisecond-scale course grained molecular dynamics simulations, we show that the model lipid bilayer can accommodate a higher concentration of triolein (TO) than earlier anticipated, by sequestering...

  9. Large-Scale Electron Microscopy Maps of Patient Skin and Mucosa Provide Insight into Pathogenesis of Blistering Diseases

    NARCIS (Netherlands)

    Sokol, Ena; Kramer, Duco; Diercks, Gilles F. H.; Kuipers, Jeroen; Jonkman, Marcel F.; Pas, Hendri H.; Giepmans, Ben N. G.

    2015-01-01

    Large-scale electron microscopy ("nanotomy") allows straight forward ultrastructural examination of tissue, cells, organelles, and macromolecules in a single data set. Such data set equals thousands of conventional electron microscopy images and is freely accessible (www.nanotomy.org). The software

  10. Apoptolysis: a novel mechanism of skin blistering in pemphigus vulgaris linking the apoptotic pathways to basal cell shrinkage and suprabasal acantholysis

    DEFF Research Database (Denmark)

    Grando, S.A.; Bystryn, J.C.; Chernyavsky, A.I.;

    2009-01-01

    ) dissociation of interdesmosomal adhesion complexes caused by phosphorylation of adhesion molecules. (4) Massive cleavage of cellular proteins by activated cell death enzymes leading to cell collapse, and tearing off desmosomes from the cell membrane stimulating secondary autoantibody production. (5) Rounding...

  11. Determination of the thickness of plastic sheets used in blister packaging by near infrared spectroscopy: development and validation of the method.

    Science.gov (United States)

    Laasonen, Magali; Harmia-Pulkkinen, Tuulikki; Simard, Christine; Räsänen, Markku; Vuorela, Heikki

    2004-03-01

    A near infrared (NIR) quantitative analysis method was developed for determining the thickness of PVC-based plastic sheets used as pharmaceutical packs. Samples that can be analyzed are transparent films made of polyvinyl chloride (PVC), PVC coated with polyvinylidene dichloride (PVDC) or PVC coated with Thermoelast (TE) and PVDC. The method, based on a partial least squares (PLS) algorithm, is used together with a previously developed NIR identification method to acquire simultaneously qualitative and quantitative information about the samples. Validation of the quantitative method was conducted according to the very recent European Agency for the Evaluation of Medicinal Products (EMEA) guidance on the use of NIR spectroscopy. Suggestions were made for a better statistical evaluation of the calibration model prior to validation. Validation consisted of the study of specificity, accuracy (mean recovery from the reference values was 99.56%), precision (repeatability and intermediate precision were <0.6%), linearity, quantification limit (41 microm), and robustness of the method. This demonstration of the applicability of NIR spectroscopy as a validated quality control tool for pharmaceutical packaging films will hopefully facilitate the acceptance of NIR spectroscopy in pharmaceutical laboratories.

  12. 原子荧光测CNAS粗铜样品中As、Sb、Bi%Atomic Fluorescence Spectrometry for Arsenic, Antimony and Bismuth Elements in CNAS Sample of Blister Copper

    Institute of Scientific and Technical Information of China (English)

    陈红; 黄惠珠

    2010-01-01

    探讨CNAS粗铜考核样中As、Sb、Bi量的测定方法,用1+1的硝酸溶解,原子荧光测定.研究了影响测定干扰元素的消除,结果准确,加标回收率为99.3%~101%,且方法简便、快速.

  13. Pimecrolimus Topical

    Science.gov (United States)

    ... rash itching swelling of the face, throat, tongue, lips, eyes, hands, feet, ankles, or lower legs difficulty breathing or swallowing crusting, oozing, blistering or other signs of skin infection cold sores chicken pox or other blisters swollen ...

  14. Sunburn: Treatment and Prevention

    Science.gov (United States)

    ... painful. In severe cases it may cause blistering, fever , chills, headache, and a general feeling of illness. Your ... age and weight.) If the sunburn causes blisters, fever, chills, headache, or a general feeling of illness, call ...

  15. What Can Be Done about Shingles?

    Medline Plus

    Full Text Available ... shingles, a skin disease that causes blisters and pain. Dr. Marques: Shingles appears as a rash and ... be on the face. Announcer: To treat the pain and the blisters from shingles, doctors currently prescribe ...

  16. Floating Carpets and the Delamination of Elastic Sheets

    KAUST Repository

    Wagner, Till J. W.

    2011-07-22

    We investigate the deformation of a thin elastic sheet floating on a liquid surface and subject to a uniaxial compression. We show that at a critical compression the sheet delaminates from the liquid over a finite region forming a delamination "blister." This blistering regime adds to the wrinkling and localized folding regimes that have been studied previously. The transition from wrinkled to blistered states occurs when delamination becomes energetically favorable compared with wrinkling. We determine the initial blister size and the evolution of blister size with continuing compression before verifying our theoretical results with experiments at a macroscopic scale. © 2011 American Physical Society.

  17. Surface morphology and deuterium retention in tungsten exposed to high flux D plasma at high temperatures

    Energy Technology Data Exchange (ETDEWEB)

    Jia, Y.Z. [Laboratory of Advanced Materials, School of Materials Science and Engineering, Tsinghua University, Beijing 100084 (China); De Temmerman, G. [FOM Institute DIFFER-Dutch Institute for Fundamental Energy Research, Edisonbaan 14, 3439 MN Nieuwegein (Netherlands); ITER Organization, Route de Vinon-sur-Verdon-CS90046, 13067 St Paul Lez Durance Cedex (France); Luo, G.-N. [Institute of Plasma Physics, Chinese Academy of Sciences, Hefei, Anhui 230031 (China); Xu, H.Y. [Science and Technology on Surface Physics and Chemistry Laboratory, Mianyang, Sichuan 621907 (China); Li, C.; Fu, B.Q. [Laboratory of Advanced Materials, School of Materials Science and Engineering, Tsinghua University, Beijing 100084 (China); Liu, W., E-mail: liuw@mail.tsinghua.edu.cn [Laboratory of Advanced Materials, School of Materials Science and Engineering, Tsinghua University, Beijing 100084 (China)

    2015-02-15

    Surface modifications and deuterium retention induced in tungsten by high fluxes (10{sup 24} m{sup −2} s{sup −1}) low energy (38 eV) deuterium ions were studied as a function of surface temperature. Blister formation was studied by scanning electron microscopy and electron backscatter diffraction, while deuterium retention was measured by thermal desorption spectroscopy. Blisters are observed on the surface exposed at different temperatures, ranging from 493 K to 1273 K. The blister density and D retention decrease with the increasing exposure temperature. The formation of blisters at high temperatures is attributed to the high flux of D plasma. At 943 K, with the increasing fluence, there is trend to the saturation of D retention and blister density. The defects caused by plasma exposure have an important effect on the D trapping and blistering behavior. The formation of blisters has a strong relationship with slipping system of tungsten.

  18. Increased Levels of Type I and III Collagen and Hyaluronan in Scleroderma Skin

    DEFF Research Database (Denmark)

    Søndergaard, Klaus; Heickendorff, Lene; L, Risteli;

    1997-01-01

    The aminoterminal propeptide of type III procollagen (PIIINP) and the carboxyterminal propeptide of type I procollagen (PICP) and hyaluronan (HA) were measured in plasma and suction blister fluid from 13 systemic sclerosis patients and 11 healthy volunteers. Suction blisters and skin biopsies were...... from the transition zone between normal skin and scleroderma, and uninvolved abdominal skin of patients. The median value of suction blister PIIINP from the transition zone was 38% higher than suction blister PIIINP from uninvolved skin. PIIINP was localized to the dermis by immunohistochemical...... techniques. PICP and HA levels in blisters from the transition zone were 87% and 53%, respectively, above the levels measured in uninvolved skin. Furthermore, PICP and HA blister levels from the transition zone were 67% and 63%, respectively, higher than the levels measured in healthy volunteers. In plasma...

  19. Human autoantibodies against a desmosomal core protein in pemphigus foliaceus

    OpenAIRE

    1984-01-01

    Pemphigus foliaceus (PF) is a human autoimmune disease in which antibodies are directed against the cell surface of epidermal cells with resultant blister formation. The histopathology of these blisters indicates that cells have detached from each other, and electron microscopy of early blisters shows diminished numbers, to complete loss, of desmosomes as well as abnormalities of the tonofilament- desmosome complex. In this study we demonstrate that autoantibodies from certain PF patients bin...

  20. Freestanding single-crystalline magnetic structures fabricated by ion bombardment

    Energy Technology Data Exchange (ETDEWEB)

    Schoenherr, P.; Bischof, A.; Boehm, B.; Eib, P.; Grimm, S.; Gross, L.; Allenspach, R., E-mail: ral@zurich.ibm.com [IBM Research – Zurich, 8803 Rüschlikon (Switzerland); Alvarado, S. F. [Department of Materials, ETH Zürich, 8093 Zürich (Switzerland)

    2015-01-19

    Starting from an ultrathin Fe film grown epitaxially on top of a GaAs(001) substrate, we show that freestanding structures can be created by ion-beam treatment. These structures are single-crystalline blisters and only a few nanometers thick. Anisotropic stress in the rim of a blister induces magnetic domain states magnetized in the direction normal to the blister edge. Experimental evidence is provided that the lateral size can be confined by starting from a nanostructured template.

  1. Degradation research of protective coating on AZ91D Mg alloy components via simulated contamination

    Directory of Open Access Journals (Sweden)

    Bao-sheng Liu

    2016-09-01

    Full Text Available Accelerated environmental (hygrothermal exposure experiments are performed on organic paints coated on commercial die-cast AZ91D Mg alloys to investigate the effects of contamination on blistering. Specifically, artificial human perspiration spray is used to contaminate the substrate surface. Blistering occurred only for paints that are spread on surfaces with the perspiration present. More blisters gradually form at longer test times, and the volume of blistering increases. Scanning electron microscopy indicates that blistering is initiated by contamination and/or substrate corrosion at the interface of the organic paints and the substrate. Blistering is characterized for two samples exposed to the hygrothermal environments for various times, and is found to be initially empty in the early stages. Hydrophilic chloride contaminants from the perspiration lead to in situ adhesion loss. Simultaneously, the paints volume expands, and the associated compressive stress causes it to bulge. After long-term test exposure, chloride anions corrode the substrate under the films, and MgO, Mg(OH2, and Mg2(OH3Cl corrosion products fill the blisters. Finally, a model of blistering evolution is discussed.

  2. "I've got a UFO stuck in my throat!"--an interesting case of foreign body impaction in the oesophagus.

    Science.gov (United States)

    Yip, L W; Goh, F S; Sim, R S

    1998-03-01

    This is a case report of an elderly lady with odynophagia because she accidentally swallowed a tablet which was still wrapped in its blister pack. A discussion of foreign body ingestion, particularly in the elderly, is included. To the authors' knowledge, this is the first paper that includes a lateral cervical radiograph of an ingested blister pack.

  3. Pre and post garter spring repositioning ultrasonic inspection of pressure tubes

    International Nuclear Information System (INIS)

    This paper present a description of the ultrasonic cracked hydride blister detections system used for pre and post inspection of pressure tubes during garter spring repositioning in CNE (Embalse Nuclear Power Station). Ultrasonic system setup configuration, transducers characteristics, blister detection head, calibration of parameters, operating procedure, records of ultrasonic inspections and evaluation. (author)

  4. Practical work of adhesion of polymer coatings studied by laser induced delamination

    NARCIS (Netherlands)

    Federov, A.V.; de Hosson, J.T.M.; van Tijum, R.; Vellinga, W.P; Buchheit, TE; Minor, AM; Spolenak, R; Takashima, K

    2005-01-01

    Laser Induced Delamination is a novel technique aimed at measuring the practical work of adhesion of thin polymer coatings on metal substrates. In this technique a laser pulse is used to create initial blisters which initiate further delamination of the film under the blister pressure. A simple elas

  5. The needs of parents with children suffering from lethal epidermolysis bullosa

    NARCIS (Netherlands)

    Yuen, W. Y.; Duipmans, J. C.; Jonkman, M. F.

    2012-01-01

    Background Some subtypes of the heterogeneous genetic blistering disease epidermolysis bullosa (EB) lead to lethality in childhood. The severity and extent of blistering leaves these patients living in excruciating pain and distress their entire lives. Parents of these patients experience some speci

  6. The effects of packaging on the stability of a moisture sensitive compound.

    Science.gov (United States)

    Allinson, J G; Dansereau, R J; Sakr, A

    2001-06-19

    Packages that provided stability (less than a 10% loss in potency) of a moisture sensitive compound (PGE-7762928) in tablet form at accelerated conditions for 6 months were identified. The equilibrium moisture content of the tablets at 25 degrees C/60%RH, 30 degrees C/60%RH and 40 degrees C/75%RH were 2.3,2.4, and 2.9%, respectively. The tablet equilibrium moisture content, degradation rate of unpackaged product, and the moisture barrier properties of the packages were used to predict the stability of the packaged product. The physical and chemical stability (HPLC assay) of the products were measured after 2,4,6,8,12, and 24 weeks at ICH conditions. The Containers-Permeation(1) of polyvinyl chloride blisters, cyclic olefin blisters, aclar blisters, cold-form aluminum blisters was 0.259, 0.040, 0.008 and 0.001 mg per blister per day, respectively. At 6 months at 40 degrees C/75%RH, the percent active was 84% in polyvinyl chloride blisters, 91% in cyclic olefin blisters, 97% in aclar blisters, 100% in cold-form aluminum blisters and 99% in an high density polyethylene bottle with a foil induction seal. The stability results for the packaged product were fairly consistent with the predictions based on the moisture sensitivity of the product and the moisture barrier properties of the respective package. To gain a better prediction, the flux value determined by the Containers-Permeation procedure was adjusted for the internal moisture concentration of the blister. PMID:11397566

  7. Localized bullous pemphigoid: report of a case with an immunofluorescence and electron microscopical studies on the lesional distribution of 180-KD bullous pemphigoid antigen, beta 4 integrin, and type VII collagen.

    Science.gov (United States)

    Kitajima, Y; Suzuki, M; Johkura, Y; Yaoita, H

    1993-07-01

    A 67-year-old woman with a left-sided hemiplegia had localized bullous pemphigoid demonstrating typical clinical lesions on the left pretibial skin and the radial-side skin of the right forearm. The histology showed a subepidermal blister with extensive hyperkeratosis, hypergranulosis, and acanthosis. Direct immunofluorescence revealed distinct linear deposits of IgG and C3 at the dermo-epidermal junction in the perilesional skin and in the roof of the blisters, but few deposits in nonlesional skin. Electron microscopy revealed separation in the lamina lucida. Indirect immunofluorescence of type VII collagen showed its localization in the blister floor. The distribution of the 180-KD bullous pemphigoid antigen (BPA) and beta 4 integrin, hemidesmosomal transmembrane proteins, were studied in the lesional skin by indirect immunofluorescence. Both 180-KD BPA and beta 4 integrin were localized in the blister roof. By immunoelectron microscopy, beta 4 integrin was detected in small groups on the cell surface facing the blister cavity. Since the epitope of the monoclonal antibody to 180-KD BPA used here is known to be localized at a distance of 20 to 50 nm from the membrane surface and this epitope retained in the blister roof, it appears that the blister was produced in the deep lamina lucida. The lesions were cleared with topical 0.05% clobetasole propionate ointment.

  8. Measuring Patient Adherence to Malaria Treatment: A Comparison of Results from Self-Report and a Customised Electronic Monitoring Device.

    Directory of Open Access Journals (Sweden)

    Katia Bruxvoort

    Full Text Available Self-report is the most common and feasible method for assessing patient adherence to medication, but can be prone to recall bias and social desirability bias. Most studies assessing adherence to artemisinin-based combination therapies (ACTs have relied on self-report. In this study, we use a novel customised electronic monitoring device--termed smart blister packs--to examine the validity of self-reported adherence to artemether-lumefantrine (AL in southern Tanzania.Smart blister packs were designed to look identical to locally available AL blister packs and to record the date and time each tablet was removed from packaging. Patients obtaining AL at randomly selected health facilities and drug stores were followed up at home three days later and interviewed about each dose of AL taken. Blister packs were requested for pill count and extraction of smart blister pack data.Data on adherence from both self-report verified by pill count and smart blister packs were available for 696 of 1,204 patients. There was no difference between methods in the proportion of patients assessed to have completed treatment (64% and 67%, respectively. However, the percentage taking the correct number of pills for each dose at the correct times (timely completion was higher by self-report than smart blister packs (37% vs. 24%; p<0.0001. By smart blister packs, 64% of patients completing treatment did not take the correct number of pills per dose or did not take each dose at the correct time interval.Smart blister packs resulted in lower estimates of timely completion of AL and may be less prone to recall and social desirability bias. They may be useful when data on patterns of adherence are desirable to evaluate treatment outcomes. Improved methods of collecting self-reported data are needed to minimise bias and maximise comparability between studies.

  9. Lionfish envenomation of the hand.

    Science.gov (United States)

    Patel, M R; Wells, S

    1993-05-01

    Lionfish (Pterois volitans) envenomation of the hand causes excruciating pain and occurs in three grades: (1) erythematous reaction, (2) blister formation, and (3) dermal necrosis. The initial treatment in all cases is to soak the hand in nonscalding water (45 degrees C) until the pain subsides by denaturing the thermolabile venom proteins. The blisters should be immediately excised to prevent dermal necrosis, inasmuch as the blister fluid contains residual active venom. To prevent a hypersensitivity reaction, any further contact with the fish should be avoided. PMID:8515029

  10. Bug bites and stings: When to see a dermatologist

    Medline Plus

    Full Text Available ... hair, and nail care Skin care Hair care / hair loss Injured skin Nail care Younger skin Kids’ zone ... hair, and nail care Skin care Hair care / hair loss Injured skin Blisters Bug bites and stings Frostbite ...

  11. Saccharomyces boulardii

    Science.gov (United States)

    ... bowel syndrome. Some people use Saccharomyces boulardii for lactose intolerance, urinary tract infections (UTIs), vaginal yeast infections, high ... cholesterol. Lyme disease. Hives. Fever blisters. Canker sores. Lactose intolerance. Other conditions. More evidence is needed to rate ...

  12. What Can Be Done about Shingles?

    Medline Plus

    Full Text Available ... have had a bout of shingles, a skin disease that causes blisters and pain. Dr. Marques: Shingles ... shingles, that can help the resolution of the disease. Announcer: Shingles can cause great discomfort and disruption ...

  13. Problems with Legs and Feet

    Science.gov (United States)

    ... ON THIS TOPIC Arthritis Why Does My Foot Fall Asleep? Word! Gait Abnormality Why Do Feet Stink? Blisters, Calluses, and Corns Going to a Physical Therapist Athlete's Foot Scoliosis Cool Cast Facts Osgood-Schlatter ...

  14. Acute generalized exanthematous pustulosis caused by morphine, confirmed by positive patch test and lymphocyte transformation test

    NARCIS (Netherlands)

    Kardaun, Sylvia H.; de Monchy, Jan G.

    2006-01-01

    Morphine, an opium alkaloid, frequently causes side effects such as hyperhidrosis and facial flushing, but serious cutaneous adverse drug reactions are seldom observed. Best known are Urticaria, erythema, and pruritus; sometimes pseudoallergic anaphylactoid reactions, and blisters are reported.

  15. Cold Sores (Orofacial Herpes)

    Science.gov (United States)

    ... rash and rashes clinical tools newsletter | contact Share | Cold Sores (Orofacial Herpes) Information for adults A A ... face, known as orofacial herpes simplex, herpes labialis, cold sores, or fever blisters, is a common, recurrent ...

  16. Coping with Cold Sores

    Science.gov (United States)

    ... Here's Help White House Lunch Recipes Coping With Cold Sores KidsHealth > For Kids > Coping With Cold Sores ... sore." What's that? Adam wondered. What Is a Cold Sore? Cold sores are small blisters that is ...

  17. Current Treatments Available for Scleroderma Patients

    Science.gov (United States)

    ... drugs have caused malformations in fetal development of rodents and rabbits. For more information, see the product ... Cyclophosphamide in Immunosuppressants). Side effects: Thinned or brittle hair, darkened and thickened skin, blistering skin or acne, ...

  18. Soluble intercellular adhesion molecule-1 (sICAM-1) and soluble interleukin-2 receptors (sIL-2R) in scleroderma skin

    DEFF Research Database (Denmark)

    Søndergaard, Klaus; Deleuran, Mette; Heickendorff, Lene;

    1998-01-01

    In order to investigate whether soluble intercellular adhesion molecule-1 (sICAM-1) and soluble interleukin-2 receptors (sIL-2R) were present in scleroderma skin, and to compare their levels to concentrations measured in plasma and clinical parameters, we examined suction blister fluid and plasma...... from 13 patients with systemic sclerosis and 11 healthy volunteers. Suction blisters and biopsies were from the transition zone between normal skin and scleroderma, and uninvolved abdominal skin. The levels of sICAM-1 and sIL-2R were significantly increased in both plasma and suction blister fluid from...... systemic sclerosis patients compared with healthy volunteers. ICAM-1 was localized to vessels and perivascular mononuclear infiltrates by immunohistochemical methods. IL-2R was expressed by CD3-positive cells. The elevated levels of sICAM-1 and sIL-2R in suction blister fluid point towards activation...

  19. What Can Be Done about Shingles?

    Medline Plus

    Full Text Available ... treat the pain and the blisters from shingles, doctors currently prescribe antiviral and pain medication. It is ... as a diagnosis of shingles has been made. Doctor: If they think they have shingles, the person ...

  20. What Is Shingles?

    Medline Plus

    Full Text Available ... Announcer: Shingles usually develops in three stages -- severe pain or tingling, possibly itchy rash and blisters that ... not have complications almost everyone with shingles experiences pain. Stephen Straus, M.D.: Most individuals over age ...

  1. Micro-scale delaminating and buckling of thin film on soft substrate

    Science.gov (United States)

    Wu, Dan; Xie, Huimin; Yin, Yajun; Tang, Minjin

    2013-03-01

    In this paper, a simple process is suggested to estimate the interfacial toughness of the material system ‘aluminum film/soft PDMS substrate’. The specimen, i.e. the aluminum film deposited on the soft polydimethylsiloxane (PDMS) substrate, is subject to a tensile load, and delaminating and buckling of aluminum film are observed in the perpendicular direction to the tensile strain. With the aid of the buckling blisters, the interfacial toughness of the material system is estimated. Large deformation is considered during the buckling of the thin film, and the interfacial toughness is deduced from a fracture theory. Besides, the evolution from one single blister to three blisters and then four blisters is observed in situ under microscope. This simplified method has potential applications to flexible electronics in which interfacial toughness of the metal film/soft substrate must be well controlled.

  2. Thermal instability of DLC film surface morphology - an AFM study

    Science.gov (United States)

    Maheswaran, R.; Thiruvadigal, D. John; Gopalakrishnan, C.

    2012-06-01

    The surface morphology of the DLC film during thermal annealing at particular temperature above the graphitization temperature shows blistering and buckling and also delaminates from the substrate. The DLC film shows poor thermal stability at higher temperature.

  3. Diabetic Neuropathy (Beyond the Basics)

    Science.gov (United States)

    ... lipoic acid — Alpha-lipoic acid (ALA) is an antioxidant medication. Several short-term trials showed that it ... find any: Redness Cuts or cracks in the skin Blisters Swelling Graphic 77032 Version 3.0 Shoe ...

  4. Disease: H00586 [KEGG MEDICUS

    Lifescience Database Archive (English)

    Full Text Available sa, junctional, with pyloric atresia (JEB-PA) Inherited epidermolysis bullosa is a diverse group of disorders characterized by mechan...ically fragile skin that readily blister. The junctional

  5. Dermatitis Herpetiformis: Skin Manifestation of Celiac Disease

    Science.gov (United States)

    ... list of Celiac Disease Organizations​​ . Alternate Language URL Dermatitis Herpetiformis: Skin Manifestation of Celiac Disease (For Health ... this page: Symptoms Causes Diagnosis Treatment Clinical Trials Dermatitis herpetiformis (DH) is a chronic, intensely itchy, blistering ...

  6. What Is Shingles?

    Medline Plus

    Full Text Available ... three stages -- severe pain or tingling, possibly itchy rash and blisters that look like chicken pox but ... Adriana Marques M.D.: The location of the rash is a clue if the rash is shingles ...

  7. Failure behavior of protective organic coatings under corrosive conditions

    Institute of Scientific and Technical Information of China (English)

    ZHANG Xian-cheng; XU Bin-shi; WANG Hai-dou; WU Yi-xiong

    2004-01-01

    Recent research activities on the formation of micro-defects and porosity in organic coatings were reviewed. The mechanisms of aggressive ionic conduction through organic coatings were analyzed. The micro-mechanisms for the failure behavior of coatings under corrosive environments were discussed in detail. These mechanisms included blistering (i. e. osmotic blistering, anodic blistering and cathodic blistering) in the coating, wet-adhesion loss at the substrate/coating interface, cathodic delamination of coating from the substrate. Based on these researches, it was found that the failure behavior of organic coatings is closely related to the micro-defects in coatings, regardless of the failure mode. Additionally, the general failure mode of a coating system was proposed to interpret the failure behavior of organic in corrosion environments. The topics discussed can provide some insights into the development of a methodology for designing fail-safe coating systems.

  8. Thermodynamic model of hydrogen-induced silicon surface layer cleavage

    International Nuclear Information System (INIS)

    A thermodynamic model of hydrogen-induced silicon surface layer splitting with the help of a bonded silicon wafer is proposed in this article. Wafer splitting is the result of lateral growth of hydrogen blisters in the entire hydrogen-implanted region during annealing. The blister growth rate depends on the effective activation energies of both hydrogen complex dissociation and hydrogen diffusion. The hydrogen blister radius was studied as a function of annealing time, annealing temperature, and implantation dose. The critical radius was obtained according to the Griffith energy condition. The time required for wafer splitting at the cut temperature was calculated in accordance with the growth of hydrogen blisters. [copyright] 2001 American Institute of Physics

  9. The Nonlinear Structure of the Desmoplakin Plakin Domain and the Effects of Cardiomyopathy-Linked Mutations

    NARCIS (Netherlands)

    C. Al-Jassar; T. Knowles; M. Jeeves; K. Kami; E. Behr; H. Bikker; M. Overduin; M. Chidgey

    2011-01-01

    Desmoplakin is a cytoplasmic desmosomal protein that plays a vital role in normal intercellular adhesion. Mutations in desmoplakin can result in devastating skin blistering diseases and arrhythmogenic right ventricular cardiomyopathy, a heart muscle disorder associated with ventricular arrhythmias,

  10. 78 FR 26784 - David Freeman: Debarment Order

    Science.gov (United States)

    2013-05-08

    ... each package. The pills were in blister packs on which was written ``Omega Biotech LTD.'' Mr. Freeman... relevant times, neither Muhi Trading Corporation nor Omega Biotech LTD. were registered to...

  11. 78 FR 25456 - Ashley Brandon Foyle: Debarment Order

    Science.gov (United States)

    2013-05-01

    ... blister packs on which was written ``Omega Biotech LTD.'' Mr. Foyle and his co-defendant, David Freeman... nor Omega Biotech LTD. were registered to manufacture, prepare, propagate, compound, or process...

  12. Molluscum contagiosum

    Science.gov (United States)

    ... lesions may result in scarring. Medicines, such as salicylic acid preparations used to remove warts , may be helpful. But these medicines can cause blistering that leads to temporary skin discoloration. Cantharidin, commonly called beetle juice, is the ...

  13. Progress towards genetic and pharmacological therapies for keratin genodermatoses: current perspective and future promise

    OpenAIRE

    Chamcheu, Jean Christopher; Wood, Gary S.; Siddiqui, Imtiaz A.; Syed, Deeba N.; Adhami, Vaqar M.; Teng, Joyce M.; Mukhtar,Hasan

    2012-01-01

    Hereditary keratin disorders of the skin and its appendages comprise a large group of clinically heterogeneous disfiguring blistering and ichthyotic diseases, primarily characterized by the loss of tissue integrity, blistering and hyperkeratosis in severely affected tissues. Pathogenic mutations in keratins cause these afflictions. Typically, these mutations in concert with characteristic features have formed the basis for improved disease diagnosis, prognosis and most recently therapy develo...

  14. Prevalence of pemphigus and pemphigoid autoantibodies in the general population

    OpenAIRE

    Prüßmann, Wiebke; Prüßmann, Jasper; Koga, Hiroshi; Recke, Andreas; Iwata, Hiroaki; Juhl, David; Görg, Siegfried; Henschler, Reinhard; HASHIMOTO, TAKASHI; Schmidt, Enno; Zillikens, Detlef; Ibrahim, Saleh M.; Ludwig, Ralf J

    2015-01-01

    Background Mucocutaneous blistering is characteristic of autoimmune bullous dermatoses (AIBD). Blisters are caused by autoantibodies directed against structural components of the skin. Hence, detection of specific autoantibodies has become a hallmark for AIBD diagnosis. Studies on prevalence of AIBD autoantibodies in healthy individuals yielded contradictory results. Methods To clarify this, samples from 7063 blood donors were tested for presence of anti-BP180-NC16A, anti-BP230 and anti-Dsg1/...

  15. Coexisting Morphea and Bullous Lichen Sclerosus Et Atrophicus: A Case Report

    OpenAIRE

    Nurcan Metin; Mahizer Yaldız; Teoman Erdem; Şahin Erdem

    2015-01-01

    Morphea and lichen skleroatrofikans (LSA)are localized fibrosing diseases characterized by well-defined fibrotic plaques. Blisters rarely occur on morphea plaque, and these blisters are mostly considered as bullous morphea which is a rare type of morphea. The cases of bullous LSA growing on plaque morphea are rarer. In this case report, we presented a patient has bullous lesions compatible with of lichen skleroatrofikans clinically and histopathologically on morphea lesions, to draw attention...

  16. Understanding effective management of postoperative wounds

    OpenAIRE

    Ousey, Karen; Roberts, Peter

    2013-01-01

    Acute surgical wounds generally heal with no problems. However, some wounds blister around the periwound area and surgical site infection postoperatively, partly due to inappropriate choice of wound dressing. These complications can cause pain, discomfort, persistent wound leakage, and possible risk of surgical site infection (Jester et al, 2000; Bhattacharyya et al, 2005; Cosker et al, 2005). Postoperative blistering and infection have been identified as the main problems in hip and knee rep...

  17. Oral Manifestations and Dental Management of Epidermolysis Bullosa Simplex

    OpenAIRE

    Scheidt, Lisa; Sanabe, Mariane Emi; Diniz, Michele Baffi

    2015-01-01

    ABSTRACT Epidermolysis bullosa (EB) is a group of hereditary chronic disorders, characterized by fragility of the skin and mucous membranes in response to minor mechanical trauma. The objective of this study was to report the case of a young girl diagnosed with epidermolysis bullosa simplex (EBS), transmitted by an autosomal dominant gene. Cutaneous findings included blisters and dystrophy following minimal friction. Recurrent blisters and vesicle formation on the hard palate were the main or...

  18. Patterned PPy Polymer and PPy/Ag Nanocomposites Thin Films by Photo-DLICVD

    OpenAIRE

    Manole, Claudiu Constantin; Maury, Francis; Demetrescu, Ioana

    2013-01-01

    This work deals with the deposition of both undoped (insulator) and extrinsically Ag-doped (conductive) polypyrrole (PPy) coatings by an original Photo-DLICVD process. The uniform and conformal coverage of PPy thin films on both Si(100) wafer and liquid micro-droplets forming blisters is investigated. A self-ordered surface patterning of the blisters is achieved and discussed in relation with the substrate nature. By changing the precursor chemistry in this CVD process, conductive PPy/Ag nano...

  19. An ecosystem-scale model for the spread of a host-specific forest pathogen in the Greater Yellowstone Ecosystem

    Science.gov (United States)

    Hatala, J.A.; Dietze, M.C.; Crabtree, R.L.; Kendall, K.; Six, D.; Moorcroft, P.R.

    2011-01-01

    The introduction of nonnative pathogens is altering the scale, magnitude, and persistence of forest disturbance regimes in the western United States. In the high-altitude whitebark pine (Pinus albicaulis) forests of the Greater Yellowstone Ecosystem (GYE), white pine blister rust (Cronartium ribicola) is an introduced fungal pathogen that is now the principal cause of tree mortality in many locations. Although blister rust eradication has failed in the past, there is nonetheless substantial interest in monitoring the disease and its rate of progression in order to predict the future impact of forest disturbances within this critical ecosystem. This study integrates data from five different field-monitoring campaigns from 1968 to 2008 to create a blister rust infection model for sites located throughout the GYE. Our model parameterizes the past rates of blister rust spread in order to project its future impact on highaltitude whitebark pine forests. Because the process of blister rust infection and mortality of individuals occurs over the time frame of many years, the model in this paper operates on a yearly time step and defines a series of whitebark pine infection classes: susceptible, slightly infected, moderately infected, and dead. In our analysis, we evaluate four different infection models that compare local vs. global density dependence on the dynamics of blister rust infection. We compare models in which blister rust infection is: (1) independent of the density of infected trees, (2) locally density-dependent, (3) locally density-dependent with a static global infection rate among all sites, and (4) both locally and globally density-dependent. Model evaluation through the predictive loss criterion for Bayesian analysis supports the model that is both locally and globally density-dependent. Using this best-fit model, we predicted the average residence times for the four stages of blister rust infection in our model, and we found that, on average, whitebark

  20. Surface and internal microstructure damage of He-ion-irradiated CLAM steel studied by cross-sectional transmission electron microscopy

    Energy Technology Data Exchange (ETDEWEB)

    Liu, P.P. [School of Material Science and Engineering, University of Science and Technology Beijing, Beijing 100083 (China); School of Metallurgical and Ecological Engineering, University of Science and Technology Beijing, Beijing 100083 (China); Zhan, Q., E-mail: qzhan@mater.ustb.edu.cn [School of Material Science and Engineering, University of Science and Technology Beijing, Beijing 100083 (China); Fu, Z.Y.; Wei, Y.P. [School of Material Science and Engineering, University of Science and Technology Beijing, Beijing 100083 (China); Wang, Y.M. [Faculty of Engineering, Hokkaido University, Sapporo 060-8628 (Japan); Wang, F.M. [School of Metallurgical and Ecological Engineering, University of Science and Technology Beijing, Beijing 100083 (China); Ohnuki, S. [Faculty of Engineering, Hokkaido University, Sapporo 060-8628 (Japan); Wan, F.R. [School of Material Science and Engineering, University of Science and Technology Beijing, Beijing 100083 (China)

    2015-11-15

    Good understanding of blistering and embrittlement mechanism depends on good investigation of surface and internal microstructure damage of gas-ion-irradiated materials. Internal and surface microstructure of He{sup +} ion irradiated CLAM steel were examined by cross-sectional transmission electron microscopy combining focused ion beam. Variation of helium bubble density and size distribution versus depth in CLAM steel after high dose helium irradiation at room temperature was investigated. The average size of helium bubble increased within 100–400 nm but decreased near the non-irradiated matrix with the increase of depth, while the density followed a reverse trend. The formation and growth mechanism of helium bubble is different at different irradiation depth. The formation of a zone of large bubbles under the surface is the main reason of surface blistering and flaking. Helium induced irradiation swelling and surface blistering at low temperature were also discussed. - Highlights: • Microstructure of helium irradiated CLAM steel was investigated by FIB and TEM. • The nucleation of helium bubble was controlled by both different mechanisms. • The substructure of surface blisters has been analyzed in detail by XTEM. • Helium induced surface blistering and irradiation swelling have been discussed.

  1. Surface and internal microstructure damage of He-ion-irradiated CLAM steel studied by cross-sectional transmission electron microscopy

    International Nuclear Information System (INIS)

    Good understanding of blistering and embrittlement mechanism depends on good investigation of surface and internal microstructure damage of gas-ion-irradiated materials. Internal and surface microstructure of He+ ion irradiated CLAM steel were examined by cross-sectional transmission electron microscopy combining focused ion beam. Variation of helium bubble density and size distribution versus depth in CLAM steel after high dose helium irradiation at room temperature was investigated. The average size of helium bubble increased within 100–400 nm but decreased near the non-irradiated matrix with the increase of depth, while the density followed a reverse trend. The formation and growth mechanism of helium bubble is different at different irradiation depth. The formation of a zone of large bubbles under the surface is the main reason of surface blistering and flaking. Helium induced irradiation swelling and surface blistering at low temperature were also discussed. - Highlights: • Microstructure of helium irradiated CLAM steel was investigated by FIB and TEM. • The nucleation of helium bubble was controlled by both different mechanisms. • The substructure of surface blisters has been analyzed in detail by XTEM. • Helium induced surface blistering and irradiation swelling have been discussed

  2. An unusual case of Sweet syndrome in a child: overlapping presentation with erythema elevatum diutinum.

    Science.gov (United States)

    Wang, Tingting; Liu, Hongjie; Wang, Lin; Guo, Zaipei; Li, Li

    2014-06-01

    In 1964, Sweet described an acute febrile neutrophilic dermatosis. It is now widely accepted that Sweet syndrome is one of the associated neutrophilic dermatoses. Herein, we describe an unusual case of Sweet syndrome in a 5-year-old child who suffered from recurrent papules, plaques, and blisters on his face and trunk after the initial onset of fever without an obvious cause. Two skin biopsies were performed. The histopathological findings of the left arm biopsy showed a subepidermal blister with dense infiltrating neutrophils within the blister that were superficial to the middle layer of the dermis. The biopsy from a plaque on the left leg showed a dense infiltrate of neutrophils and a large number of infiltrating histiocytes superficial to the middle layer of the dermis. Direct immunofluorescence of a skin biopsy from the left arm confirmed that immunoglobulin M (IgM), IgG, IgA, and C3 expression were negative.

  3. Application of clean laser transfer for porphyrin micropatterning

    International Nuclear Information System (INIS)

    Blister-based laser-induced forward transfer is proposed as a promising tool for clean, cold and liquid-free local transfer of various organic substances. The feature of the given technique is non-destructive local deformation of an absorbing metal film on a transparent support avoiding the metal sputtering. Application of the blister-based laser transfer of a Langmuir film to fabricate mesotetraphenylporphyrin micropatterns on a silica substrate has been demonstrated. The metal film thickness is found to be a key parameter, which determines the laser fluence range allowing the clean transfer, predominant mechanism of the blister formation and laser-induced heating of the transferred material. According to the numerical modelling confirmed by UV-vis absorption spectroscopy, the target with 1.5 μm thick titanium film provides negligible heating of the porphyrin transferred by 5 ns laser pulses.

  4. Thermodynamic Study on Process in Copper Converters (The Copper-making Stage)

    Institute of Scientific and Technical Information of China (English)

    2000-01-01

    Theoretical calculations were based on thermodynamic equilibrium in the multi-component and multi-phase system with heat and mass balance as well as the oxygen efficiency to take account for the effects of process kinetics. The variations of temperature, mass fractions of dissolved oxygen and sulfur in blister copper, partial pressures for O2, S2, SO2 in gas phase for the copper-making stage were calculated. The model predicted temperature, time of blowing as well as mass of the blister copper at end points for 6 heats showed a fairly good agreements with corresponding plant data. The calculated content of 0.065% and content of 0.87% in blister copper were both at reasonable levels. Compared with the so called Goto model, the present model has very much improved process description of copper-making stage as well as the prediction of end points for a copper converter by introducing the oxygen efficiency.

  5. Surface modifications of W divertor components for EAST during exposure to high heat loads with He

    Energy Technology Data Exchange (ETDEWEB)

    Li, C., E-mail: lichun10@mails.tsinghua.edu.cn [Laboratory of Advanced Materials, School of Materials Science and Engineering, Tsinghua University, Beijing 100084 (China); Greuner, H. [Max Planck Institute for Plasma Physics, Boltzmannstr. 2, 85748 Garching (Germany); Yuan, Y. [School of Physics and Nuclear Energy Engineering, Beihang University, Beijing 100191 (China); Zhao, S.X.; Luo, G.N. [Institute of Plasma Physics, Chinese Academy of Sciences, Hefei, Anhui 230031 (China); Böswirth, B. [Max Planck Institute for Plasma Physics, Boltzmannstr. 2, 85748 Garching (Germany); Fu, B.Q.; Jia, Y.Z. [Laboratory of Advanced Materials, School of Materials Science and Engineering, Tsinghua University, Beijing 100084 (China); Liu, X. [Southwestern Institute of Physics, Chengdu, Sichuan 610041 (China); Liu, W., E-mail: liuw@mail.tsinghua.edu.cn [Laboratory of Advanced Materials, School of Materials Science and Engineering, Tsinghua University, Beijing 100084 (China)

    2015-08-15

    Flat-type W/Cu plasma-facing components have been developed for the new generation divertor of the Chinese Experimental Advanced Superconducting Tokamak. Surface modifications of such actively water-cooled W components following short and long pulse high heat loading coupled with He particle loads with fluence of 3 × 10{sup 22} m{sup −2} have been investigated. An adiabatically loaded W block was investigated as a comparison and exposed to short pulse loads. Blistering was observed on all sample surfaces, but was less pronounced on the components than on the W block, due to the significant lower surface temperature caused by active cooling. For components, longer pulse loads gave rise to a rougher surface. Furthermore, most blisters on components are found to be less than 1 μm in diameter, with just a very few blisters larger than 1 μm, observed only in some near 〈1 1 1〉 grains.

  6. Effect of smoking, abstention, and nicotine patch on epidermal healing and collagenase in skin transudate

    DEFF Research Database (Denmark)

    Sorensen, L.T.; Zillmer, R.; Agren, M.;

    2009-01-01

    and in 6 never smokers. Matrix metalloproteinase (MMP)-8 and MMP-1 levels in suction blister fluid were assessed by an enzyme-linked immunosorbent assay. Random-effects models for repeated measurements were applied and p wounding the TEWL was 17.20 (14......Delayed wound healing may explain postoperative tissue and wound dehiscence in smokers, but the effects of smoking and smoking cessation on the cellular mechanisms remain unclear. Suction blisters were raised in 48 smokers and 30 never smokers. The fluid was retrieved and the epidermal roof....... Three months of abstinence from smoking does not restore epidermal healing, whereas 4 weeks of abstinence normalizes suction blister MMP-8 levels. These findings suggest sustained impaired wound healing in smokers and potential reversibility of extracellular matrix degradation Udgivelsesdato: 2009/5...

  7. Some recent observations on the radiation behavior of uranium silicide dispersion fuel

    Energy Technology Data Exchange (ETDEWEB)

    Hofman, G.L.

    1988-01-01

    Addition of B{sub 4}C burnable poison results in higher plate swelling in both U{sub 3}Si{sub 2} and U{sub 3}Si-Al dispersion fuel plates and also decreases the blister threshold temperature of these plates. Prolonged annealing of U{sub 3}Si{sub 2}-Al fuel plates produced no blister after 696 hours at 400{degrees}C. Blister formation started between 257 hours and 327 hours at 425{degrees}C and between 115 hours and 210 hours at 450{degrees}C. Operation with breached cladding resulted in pillowing of an U{sub 3}Si-Al fuel plate due to reaction of the fuel core with coolant water. 4 refs., 10 figs., 2 tabs.

  8. IMMUNOHISTOCHEMISTRY STUDIES IN A CASE OF DERMATITIS HERPETIFORMIS DEMONSTRATE COMPLEX PATTERNS OF REACTIVITY

    Directory of Open Access Journals (Sweden)

    Ana Maria Abreu Velez

    2013-11-01

    Full Text Available Introduction: Dermatitis herpetiformis (DH is an autoimmune, clinically pleomorphic, papulovesicular disorder sometimes associated with celiac disease and gluten sensitivity. DH is categorized by subepidermal vesicles and bullae on hematoxylin and eosin (H&E staining, and with immunoglobulin A deposits present along the dermal papillary tips on direct immunofluorescence (DIF. Case Report: We describe a 50 year old female that presented with sudden onset pruritus and clinical blisters, predominantly on extensor areas of the extremities. Biopsies for H&E examination, as well as immunohistochemistry (IHC and DIF analysis were performed. Results: H&E examination demonstrated subepidermal blistering; within the blister lumen, numerous neutrophils were present, with occasional eosinophils and lymphocytes also seen. DIF examination revealed linear deposits of IgA along the epidermal basement membrane zone, associated with other immunoglobulins and complement. IHC examination showed similar patterns of reactivity to IgA, and also to other immunoreactants. Cells positive for CD1a were present within the blisters, correlating with S-100 staining. Cells staining positive for CD8, CD45 and occasionally CD4 and Granzyme B were seen not only in the blister lumens, but also around neurovascular packages under the blisters. Finally, CD2 positive cells were found around the upper dermal blood vessels. Discussion: Focal DIF linear IgA deposition is the classic hallmark diagnostic finding in DH. However, it is possible that genetic susceptibility and environmental triggers also play a crucial role in the pathogenesis, often acting via cellular pathways exhibiting disease-associated polymorphisms. In tolerance breakthrough, the initiating antigen presenting cells likely lead to immune system cell differentiation, and activation of adaptive immunity.

  9. IgG/IgE bullous pemphigoid with CD45 lymphocytic reactivity to dermal blood vessels, nerves and eccrine sweat glands

    Directory of Open Access Journals (Sweden)

    Ana Maria Abreu-Velez

    2010-01-01

    Full Text Available Context: Bullous pemphigoid (BP, the most common autoimmune blistering disease, is mediated by autoantibodies. BP primarily affects the elderly and is characterized by the development of urticarial plaques surmounted by subepidermal blisters, and the deposition of immunoglobulins and complement at the basement membrane zone (BMZ of the skin. BP is immunologically characterized by the development of autoantibodies targeting two structural proteins of the hemidesmosomes, BP180 (collagen XVII and BP230 (BPAG1. Case Report: A 63 -year-old Caucasian female patient was evaluated for a 4 day history of several itching, erythematous blisters on her extremities. Biopsies for hematoxylin and eosin (H&E examination, as well as Periodic acid-Schiff (PAS, immunohistochemistry (IHC and direct immunofluorescence (DIF analysis were performed. Results: H&E demonstrated a subepidermal blister, with partial re-epithelialization of the blister floor. Within the blister lumen, numerous neutrophils were present, with occasional eosinophils and lymphocytes also noted. Within the dermis, a mild, superficial, perivascular and periadnexal infiltrate of lymphocytes, histiocytes and occasional eosinophils was identified, with mild perivascular leukocytoclastic debris. The PAS stain was positive at the BMZ, and around selected blood vessels, nerves and sweat glands. DIF revealed linear deposits of IgG and Complement/C3 and fibrinogen at the BMZ, and around selected dermal nerves, blood vessels and sweat glands. Strong granular deposits of IgE were also observed, colocalizing with monoclonal antibodies to Collagen IV (CIV. By IHC, positive CD45 staining of lymphocytes was seen surrounding selected dermal blood vessels, eccrine sweat glands, and nerves. Conclusion : The patient displayed IgG, IgE, and fibrinogen autoantibodies against the BMZ, as well as around some dermal nerves and sweat glands; their binding in the skin could trigger complement activation. In addition, the

  10. Radiosensitive Hematopoietic Cells Determine the Extent of Skin Inflammation in Experimental Epidermolysis Bullosa Acquisita.

    Science.gov (United States)

    Iwata, Hiroaki; Witte, Mareike; Samavedam, Unni Krishna S R L; Gupta, Yask; Shimizu, Atsushi; Ishiko, Akira; Schröder, Tobias; Seeger, Karsten; Dahlke, Markus; Rades, Dirk; Zillikens, Detlef; Ludwig, Ralf J

    2015-09-01

    Animal models have enhanced our understanding of the pathogenesis of autoimmune diseases. For these models, genetically identical, inbred mice have commonly been used. Different inbred mouse strains, however, show a high variability in disease manifestation. Identifying the factors that influence this disease variability could provide unrecognized insights into pathogenesis. We established a novel Ab transfer-induced model of epidermolysis bullosa acquisita (EBA), an autoimmune disease characterized by (muco)-cutaneous blistering caused by anti-type VII collagen (COL7) autoantibodies. Blistering after anti-COL7 IgG (directed against the von Willebrand factor A-like domain 2) transfer showed clear variability among inbred mouse strains, that is, severe cutaneous blistering and inflammation in C57BL/6J and absence of skin lesions in MRL/MpJ mice. The transfer of anti-COL7 IgG into irradiated, EBA-resistant MRL/MpJ mice, rescued by transplantation with bone marrow from EBA-susceptible B6.AK-H2k mice, induced blistering. To the contrary, irradiated EBA-susceptible B6.AK-H2k mice that were rescued using MRL/MpJ bone marrow were devoid of blistering. In vitro, immune complex activation of neutrophils from C57BL/6J or MRL/MpJ mice showed an impaired reactive oxygen species release from the latter, whereas no differences were observed after PMA activation. This finding was paralleled by divergent expression profiles of immune complex-activated neutrophils from either C57BL/6J or MRL/MpJ mice. Collectively, we demonstrate that radiosensitive cells determine the varying extent of skin inflammation and blistering in the end-stage effector phase of EBA. PMID:26202985

  11. Growth, straightness and survival at age 32 in a Pinus strobus x P. wallichiana F1 hybrid population (Experiment 1)

    OpenAIRE

    Ioan Blada; Cristiana Dinu; Ştefan Tănasie; Iulian Bratu

    2013-01-01

    An interspecific factorial hybridization was made, where seven P. strobus female parent trees were mated to four P. wallichiana male trees, in order to transfer genes for resistance to blister-rust from the blue pine to the F1 hybrids. The hybrid seedlings and the open pollinated families of the two parents were three times consecutively inoculated with blister-rust. The six years old seedlings were transplanted in the fi eld in the so called Valiug Experiment 1. The experimental design consi...

  12. [Acute pseudo-membranous laryngitis in epidermolysis bullosa acquisita].

    Science.gov (United States)

    Lecomte, H; Folia, M; Muller, G; Gilbert, D; Tron, F; Besancenot, J-F; Romanet, P

    2010-05-01

    Epidermolysis bullosa acquisita is a rare entity belonging to the auto-immune cutaneous blistering disorders of the dermo-epidermal junction. Clinical manifestations are generally cutaneous including the development of sub-epidermal blisters. Mucosal manifestations should be systematically looking for, but laryngeal involvement remains uncommon. We report an 81-year-old woman who presented with dysphagia, dyspnea and dysphonia as the presenting features of laryngeal involvement of an epidermolysis bullosa acquisita. This is the tenth reported case in the literature. We describe our diagnostic approach and the therapeutic management, comparing them with the literature.

  13. "Nurse's cramp"

    DEFF Research Database (Denmark)

    Jepsen, Jørgen Riis

    2015-01-01

    . Three patients with “nurse’s cramp” related to this task are presented. The patients were referred to a department of occupational medicine due to volar forearm and hand pain related to tablet-dosing from blister packs. A detailed physical examination including a neurological assessment was performed......Calculating the number of tablets or capsules to administer to patients is one of the most common tasks that a nurse is required to make. Home care and nursing home staff may dose tablets for clients for up to two hours per day. An increasing proportion of tablets are dispensed in blister packs...

  14. Congenital erythropoietic porphyria in three siblings.

    Science.gov (United States)

    Bari, Arfan Ul

    2007-01-01

    Congenital erythropoietic porphyria is a rare autosomal recessive disorder that usually presents with marked skin photosensitivity, hypertrichosis, blistering, scarring, milia formation and dyspigmentation of the photo-exposed areas. Three adult siblings (two sisters and one brother) are presented here with variable degree of skin manifestations. During early childhood, all the siblings started showing signs of photosensitivity with darkening of urine color followed by skin blistering over the face and hands. The oldest showed severe sclerodermiform mutilation and the youngest exhibited an initial involvement with hypertrichosis. None of them had any history of convulsions, acute abdominal pain or joint pain. Woods lamp examination and laboratory investigations confirmed the diagnosis. PMID:17921617

  15. Cicatricial Pemphigoid in Accompany with Rheumatoid Arthritis: a Case Report

    Institute of Scientific and Technical Information of China (English)

    Di Wu; Li-ming Zhang; Ying Jiang

    2010-01-01

    @@ CICATRICIAL pemphigoid (CP, also known as benign mucous membrane pemphigoid) is a rare chronic autoimmune subepithelial blister-ing disease, with an incidence of 1 per million, characterized by erosive lesions of mucous membranes and skin that result in scarring.1,2 Rheumatoid arthritis (RA) is a symmetric inflammatory arthritis that mainly affects the small joints of hands and feet, with a prevalence of 0.3% in China. In this case report we described the diagnosis of and treatment for a patient developing CP 18 years after the onset of RA, a combination rarely en-countered or reported so far.

  16. Coexisting Morphea and Bullous Lichen Sclerosus Et Atrophicus: A Case Report

    Directory of Open Access Journals (Sweden)

    Nurcan Metin

    2015-12-01

    Full Text Available Morphea and lichen skleroatrofikans (LSAare localized fibrosing diseases characterized by well-defined fibrotic plaques. Blisters rarely occur on morphea plaque, and these blisters are mostly considered as bullous morphea which is a rare type of morphea. The cases of bullous LSA growing on plaque morphea are rarer. In this case report, we presented a patient has bullous lesions compatible with of lichen skleroatrofikans clinically and histopathologically on morphea lesions, to draw attention to rare association of bullous morphea and LSA.

  17. The influence of the ion implantation temperature and the dose rate on smart-cut in GaAs

    International Nuclear Information System (INIS)

    The temperature and dose rate dependence of the smart-cut process in GaAs have been investigated in this paper. The distribution of hydrogen and the implantation damage in the samples were studied by ion beam analysis and X-ray diffraction. It was found that at higher temperatures, hydrogen is mobile in the lattice and can rearrange into the platelets, microcracks and bubbles which are present in blistered material, thus relieving the strain in the lattice. The dose rate was also found to be significant for the smart-cut process, as blistering and exfoliation are inhibited at low dose rates

  18. Beschreibung neuer Faktoren mit Einfluss auf das spontane Überlesen von Stopcodon-Mutationen

    OpenAIRE

    Pacho, Frederic

    2010-01-01

    Origin of this work was a patient with junctional epidermolysis bullosa (JEB). This patient, JEB-NRT, suffered from generalized blistering of skin and mucosae after birth and had dystrophic finger- and toenails, symptoms characteristic for JEB. Today, JEB-NRT is six years old and shows only occasionally localized blistering of the skin, a characteristic feature of non-Herlitz JEB. Genotype analysis of the patient JEB-NRT revealed two premature termination codons in the LAMA3 gene, R943X and R...

  19. Bullous Mastocytosis Mimicking Congenital Epidermolysis Bullosa

    Directory of Open Access Journals (Sweden)

    Julio Cesar Salas-Alanis

    2014-05-01

    Full Text Available A 2-month-old female infant was referred to DebRA Mexico from the Regional Children's Hospital because of a generalized dermatosis from birth characterized by multiple blisters and erosions on the trunk, face and limbs, associated with minor trauma. A skin biopsy showing subepidermal blisters associated with a dermal infiltrate of Giemsa-positive cells and CD117-positive antibody was consistent with the diagnosis of bullous mastocytosis. Treatment with oral antihistamines, topical steroids, and antibiotics was initiated, leading to a remission of the lesions.

  20. Epidermolysis Bullosa Acquisita: From Pathophysiology to Novel Therapeutic Options.

    Science.gov (United States)

    Kasperkiewicz, Michael; Sadik, Christian D; Bieber, Katja; Ibrahim, Saleh M; Manz, Rudolf A; Schmidt, Enno; Zillikens, Detlef; Ludwig, Ralf J

    2016-01-01

    Epidermolysis bullosa acquisita (EBA) is a prototypic organ-specific autoimmune disease induced by autoantibodies to type VII collagen causing mucocutaneous blisters. In the inflammatory (bullous pemphigoid-like) EBA variant, autoantibody binding is followed by a lesional inflammatory cell infiltration, and the overall clinical picture may be indistinguishable from that of bullous pemphigoid, the latter being the most common autoimmune bullous disease. The last decade witnessed the development of several mouse models of inflammatory EBA that facilitated the elucidation of the pathogenesis of autoantibody-induced, cell-mediated subepidermal blistering diseases and identified new therapeutic targets for these and possibly other autoantibody-driven disorders. PMID:26763420

  1. Analysis of vesicle fluid following the sting of the lionfish Pterois volitans.

    Science.gov (United States)

    Auerbach, P S; McKinney, H E; Rees, R S; Heggers, J P

    1987-01-01

    Fluid aspirated from blisters following a lionfish (Pterois volitans) sting was analyzed utilizing combined capillary column gas chromatography and negative ion chemical ionization mass spectrometry. Analysis for prostaglandin F2 alpha demonstrated 16.91 ng/ml, for prostaglandin E2 0.143 ng/ml, for 6-keto-prostaglandin F1 alpha less than 0.1 ng/ml (nondetectable) and for thromboxane B2 1.65 ng/ml. Platelet aggregation studies showed that blister fluid caused aggregation of isolated platelets only, which was inhibited by heat treatment or by the presence of normal donor plasma. PMID:3438924

  2. Staphylococcal scalded skin syndrome : loss of desmoglein 1 in patient skin

    NARCIS (Netherlands)

    Aalfs, A. Susanne; Oktarina, D. A. Mira; Diercks, Gilles F. H.; Jonkman, Marcel F.; Pas, Hendri H.

    2010-01-01

    Staphylococcal scalded skin syndrome (SSSS) is a blistering disease of the skin caused by an infection with certain strains of Staphylococcus aureus. In vitro studies have suggested that exfoliative toxins secreted by these bacteria cleave the desmosomal adhesion molecule desmoglein 1 leading to los

  3. When the Going Gets Tough...

    Institute of Scientific and Technical Information of China (English)

    CORRIE; DOSH

    2007-01-01

    An American discovers China in a 250-km race across the Gobi Desert Evening was fading into night, and after jogging five days across blistering hot dunes treacherous river cross- ings and mountains, Jim Chow could run no longer. Knees failing,

  4. Children with Rare Chronic Skin Diseases: Hemangiomas and Epidermolysis Bullosa.

    Science.gov (United States)

    Jones, Sheila Dove; Miller, Cynthia Dieterich

    The paper reports on studies involving children having the rare chronic skin diseases of hemangiomas and epidermolysis bullosa (characterized by easy blistering). One study compared the self-concept and psychosocial development of young (mean age 46 months) children (N=19) with hemangiomas with 19 children without hemangiomas. Findings indicated…

  5. Sacral Herpes

    Science.gov (United States)

    ... to her newborn baby. HSV-2 is often spread through direct skin-to-skin contact with active lesions on another person. However, people who have ... important to take the following steps to prevent spread (transmission) of the virus ... or itching) and active phase (presence of blisters or sores) of HSV- ...

  6. What Can Be Done about Shingles?

    Medline Plus

    Full Text Available [ music ] Announcer: By the time they reach 80, 50% of Americans will have had a bout of shingles, a skin disease that causes blisters and ... vaccine. Irvin: The discomfort is so intense at times that you almost wish that you don't ...

  7. HLA-A*3101 and carbamazepine-induced hypersensitivity reactions in Europeans.

    LENUS (Irish Health Repository)

    McCormack, Mark

    2011-03-24

    Carbamazepine causes various forms of hypersensitivity reactions, ranging from maculopapular exanthema to severe blistering reactions. The HLA-B*1502 allele has been shown to be strongly correlated with carbamazepine-induced Stevens-Johnson syndrome and toxic epidermal necrolysis (SJS-TEN) in the Han Chinese and other Asian populations but not in European populations.

  8. Two major 5 '-untranslated regions for type XVII collagen mRNA

    NARCIS (Netherlands)

    van Zalen, Sebastiaan; Nijenhuis, Albertine; Jonkman, Marcel F.; Pas, Hendri H.

    2006-01-01

    Background: Type XVII collagen is an important structural component of keratinocyte hemidesmosomes and its functional loss in genetic or autoimmune disease results in blistering of the skin. In neoplastic tissue aberrant expression is seen dependent on the stage of the tumor. White the sequence of t

  9. Foot-and-Mouth Disease

    DEFF Research Database (Denmark)

    Belsham, Graham; Charleston, Bryan; Jackson, Terry;

    2015-01-01

    Foot‐and‐mouth disease (FMD) is an economically important, highly contagious disease of cloven‐hoofed animals characterised by the appearance of vesicles (blisters) on the feet and in, and around, the mouth. The causative agent, foot‐and‐mouth disease virus (FMDV), was the first mammalian virus...

  10. Foot-and-mouth disease

    DEFF Research Database (Denmark)

    Belsham, Graham; Charleston, Bryan; Jackson, Terry;

    2009-01-01

    Foot-and-mouth disease is an economically important, highly contagious, disease of cloven-hoofed animals characterized by the appearance of vesicles (blisters) on the feet and in and around the mouth. The causative agent, foot-and-mouth disease virus, was the first mammalian virus to be discovered...

  11. Idelalisib

    Science.gov (United States)

    ... treatment: chills, fever, cough, or other signs of infection painful, frequent, or difficult urination swelling of the hands, feet, ankles, or lower legs red, peeling, or blistering skin painful sores or ulcers on your skin, lips, or in your mouth difficulty swallowing or breathing ...

  12. Nevirapine

    Science.gov (United States)

    ... fever;sore throat, chills, or other signs of infection; flu-like symptoms; muscle or joint aches; blisters; mouth sores; red or swollen eyes; hives; itching; swelling of the face, throat, tongue, lips, eyes, hands, feet, ankles, or lower legs; hoarseness; or difficulty breathing or swallowing.If ...

  13. Kindler′s syndrome: A rare case report

    Directory of Open Access Journals (Sweden)

    Neelam Suman

    2014-01-01

    Full Text Available Kindler syndrome is a rare hereditary disorder, associated with skin fragility. The syndrome involves the skin and mucous membrane with radiological changes. The genetic defect has been identified on the short arm of chromosome 20. This report describes a 16-year-old patient with classical features like blistering and photosensitivity in childhood and the subsequent development of poikiloderma.

  14. Degradation mechanisms of organic rubber and glass flake/vinyl ester linings in flue gas desulphurization plants

    Energy Technology Data Exchange (ETDEWEB)

    Weber, R. [Allianz-Centre for Technology GmbH, Ismaning (Germany)

    1996-12-01

    In recent years, there have been reports in numerous publications about damage to rubber and glass flake coatings in flue gas desulphurization plants. The pattern of damage has been described and attempts have frequently been made to determine and explain the cause of the damage. Oxidation/hydrolytic changes were generally observed as the damage mechanisms. In addition, blistering occurs in both the chloroprene coatings in the absorbers and in the glass flake coatings in clean gas ducts. This blistering may be considered as the end of the useful life and leads to cost-intensive and time-intensive repair and restoration measures. The present state of knowledge suggests that the blistering is mainly due to osmotic processes preceded by permeation processes and with permeation processes superimposed on them. Among other things, the reports describe the permeation behaviour of water and other flue gas constituents; the blistering in chloroprene rubber coatings and glass flake coatings is explained by means of the knowledge gained. (au) 16 refs.

  15. Chinese Scientists Starting Hand Foot and Mouth Disease Vaccine

    Institute of Scientific and Technical Information of China (English)

    2013-01-01

    <正>The infection causes a rash and painful blisters(水疱),but in some cases results in brain infections which can be fatal.A trial involving 10,000 children,published in the Lancet,showed the vaccine was 90% effective against one virus which causes the disease.

  16. Spider Bites (For Parents)

    Science.gov (United States)

    ... brown recluse spider bite: red blister in the center with surrounding bluish discoloration around the bite swelling or redness around the bite development of pain around the bite within 2 to 8 hours joint stiffness or pain nausea, vomiting body rash fever and ...

  17. Liposuction sepsis – be alert

    OpenAIRE

    Bell, Michael SG; Doherty, Geoffrey P; Gutauskas, Andrea

    2009-01-01

    Postoperative infection in liposuction patients may be exceedingly difficult to clinically appreciate because of the skin edema, colour changes and even blistering that can occur. The authors outline such a case with practical advice about establishing an accurate and precise diagnosis of an early septic process.

  18. Audio 2008--Alternative Rock: Forced Exposure Is Good for You

    Science.gov (United States)

    Pasteur, Eric

    2008-01-01

    According to this author, alternative rock did not deserve what it became in the post-Nirvana 1990s. Reduced to a marketing catch-phrase--effectively rendering it meaningless--major label co-optation helped blur the line between artistic expression and cloned sounds. Before Nirvana broke through to the mainstream with the blistering sarcasm of its…

  19. Oral purpura as the first manifestation of primary systemic amyloidosis.

    Science.gov (United States)

    McCormick, Robert Stuart; Sloan, Philip; Farr, David; Carrozzo, Marco

    2016-07-01

    Oral blood blisters and purpura are rare features of primary systemic amyloidosis (amyloid light-chain (AL) amyloidosis). We report a case in which these unusual presentations led to a diagnosis of amyloidosis, which enabled effective treatment before organ failure. PMID:26708800

  20. Work of adhesion in laser-induced delamination along polymer-metal interfaces

    NARCIS (Netherlands)

    Fedorov, A.; van Tijum, R.; Vellinga, W. P.; de Hosson, Jeff

    2007-01-01

    Laser-induced delamination is a recent technique aimed at characterizing adhesive strength of thin polymer coatings on metal substrates. A laser pulse is used to create a blister that initiates further delamination of the film under pressure. To process the experimental data a simple elastic model w

  1. Experimental Study of Heat Dissipation in Indoor Sports Shoes

    NARCIS (Netherlands)

    Dessing, O.; Jansen, A.J.; Leihitu, C.; Overhage, D.

    2014-01-01

    As indoor sports shoes are intensively used in a warm and sweaty environment for periods of up to three consecutive hours, the built-up heat inside is insufficiently released causing warm and perspiring feet. This results in an increased chance of blisters and skin irritations. Experimental research

  2. Salt and pepper staining patterns for LAT, ZAP-70 and MUM-1 in a vasculitic bullousallergic drug eruption

    Directory of Open Access Journals (Sweden)

    Abreu Velez Ana Maria

    2011-07-01

    Full Text Available Background. The term bullous drug eruption refers to clinically adverse drug reactions that result in fluid-filled blisters or bullae. Blistering can be elicited by multiple medications, prescribed or over-the-counter, natural or synthetic. Case Report: A 78-year-old female was evaluated for the presence of a rapidly appearing, diffuse rash with vesicles, bullae and abdominal edema. Methods: Skin biopsies for hematoxylin and eosin examination, as well as for direct immunofluorescence and immunohistochemistry analysis were performed. Results: H&E staining demonstrated a subepidermal blistering disorder. Within the dermis, a mild, superficial, perivascular infiltrate of lymphocytes, histiocytes and eosinophils was seen. No frank leukocytoclastic vasculitis was appreciated. Direct immunofluorescence revealed a strong presence of Complement/C3, IgM and fibrinogen in the upper dermal blood vessels. Staining with LAT, MUM-1, and ZAP-70 was identified in the inflamed vessels, in a delicate salt and pepper pattern. Conclusions: In bullous drug eruptions, inflammation of the dermal blood vessels without frank leuckocytoclasis is often noted; vascular alterations subjacent to the blisters are frequently described as nonspecific. We document specific activation markers of the T cell immune response; further secondary cell signaling pathway molecules are overexpressed in dermal blood vessels, indicative of a complex immune response in these patients

  3. Automatic differentiation of u- and n-serrated patterns in direct immunofluorescence images

    NARCIS (Netherlands)

    Shi, Chenyu; Guo, Jiapan; Azzopardi, George; Meijer, Joost; Jonkman, Marcel F.; Petkov, Nicolai

    2015-01-01

    Epidermolysis bullosa acquisita (EBA) is a subepidermal autoimmune blistering disease of the skin. Manual u- and n-serrated patterns analysis in direct immunofluorescence (DIF) images is used in medical practice to differentiate EBA from other forms of pemphigoid. The manual analysis of serration pa

  4. Automatic Classification of Serrated Patterns in Direct Immunofluorescence Images

    NARCIS (Netherlands)

    Shi, Chenyu; Meijer, Joost; Guo, Jiapan; Azzopardi, George; Jonkman, Marcel F.; Petkov, Nicolai

    2015-01-01

    Direct immunofluorescence (DIF) images are used by clinical experts for the diagnosis of autoimmune blistering diseases. The analysis of serration patterns in DIF images concerns two types of patterns, namely n- and u-serrated. Manual analysis is time-consuming and challenging due to noise. We propo

  5. Ultraviolet-B-driven pigmentation and genetic diversity of benthic macroinvertebrates from high-altitude Andean streams

    NARCIS (Netherlands)

    R.A. Loayza Muro; J.K. Marticorena-Ruíz; E.J. Palomino; C Merrit; J.A.J. Breeuwer; P. Kuperus; M.H.S. Kraak; W. Admiraal

    2013-01-01

    Photoprotective pigments in benthic macroinvertebrates may reduce the damage caused by the blistering UV-B radiation in Andean high-altitude streams above 3500 m. The aim of this study was therefore to determine whether melanisation in macroinvertebrates inhabiting high-altitude Andean streams is an

  6. What Is Shingles?

    Science.gov (United States)

    ... It's caused by the same virus that causes chicken pox. About four million of us get chicken pox every year and that virus stays in the ... possibly itchy rash and blisters that look like chicken pox but unlike chicken pox shingles only affects one ...

  7. What Is Shingles?

    Medline Plus

    Full Text Available ... It's caused by the same virus that causes chicken pox. About four million of us get chicken pox every year and that virus stays in the ... possibly itchy rash and blisters that look like chicken pox but unlike chicken pox shingles only affects one ...

  8. Semi-continuous high speed gas analysis of generated vapors of chemical warfare agents

    NARCIS (Netherlands)

    Trap, H.C.; Langenberg, J.P.

    1999-01-01

    A method is presented for the continuous analysis of generated vapors of the nerve agents soman and satin and the blistering agent sulfur mustard. By using a gas sampling valve and a very short (15 cm) column connected to an on-column injector with a 'standard length' column, the system can either b

  9. Laboratory Diagnosis and Clinical Profile of Anti-p200 Pemphigoid

    NARCIS (Netherlands)

    Meijer, Joost M; Diercks, Gilles F H; Schmidt, Enno; Pas, Hendri H; Jonkman, Marcel F

    2016-01-01

    Importance: Anti-p200 pemphigoid is a rare subepidermal autoimmune blistering disease characterized by autoantibodies against a 200-kDa protein in the basement membrane zone. Anti-p200 pemphigoid is probably often misdiagnosed because of low availability of diagnostic assays and expertise and classi

  10. Revertant cell therapy for epidermolysis bullosa

    NARCIS (Netherlands)

    Gostynski, Antoni

    2014-01-01

    Epidermolysis bullosa (EB) is an inherited skin disease. Patients have lifelong fragile skin leading to chronic blistering of the skin and mucosa. Children born with EB are called Butterfly Children, because their skin is as fragile as the wings of a butterfly. Presently, the disease has no cure. In

  11. 50,000-ton Anode Copper Project of Rongda Copper Co.,Ltd in Baise of Guangxi Has Been Put Into Operation

    Institute of Scientific and Technical Information of China (English)

    2009-01-01

    <正>Rongda Copper Co.,Ltd in Baise of Guangxi, which was restructured in May 2006,has achieved an annual capacity of 20,000 tons of blister copper,and 60,000 tons of sulfur acid. Recently,the company’s 50,000-ton anode

  12. Preparation and Properties of Hydrothermally Stable γ-Alumina Membranes

    NARCIS (Netherlands)

    Nijmeijer, Arian; Kruidhof, Henk; Bredesen, Rune; Verweij, Henk

    2001-01-01

    Supported mesoporous γ-Al2O3 membranes deteriorate and blister in steam-containing environments at high temperatures. This deterioration led us to the development of a new type of supported γ-Al2O3 membrane with significantly improved stability under hostile conditions. Two measures were taken to ac

  13. Somatic mosaicism for the COL7A1 mutation p.Gly2034Arg in the unaffected mother of a patient with dystrophic epidermolysis bullosa pruriginosa

    NARCIS (Netherlands)

    van den Akker, P. C.; Pasmooij, A. M. G.; Meijer, R.; Scheffer, H.; Jonkman, M. F.

    2015-01-01

    Dystrophic epidermolysis bullosa (DEB) is a heritable blistering disorder caused by mutations in the type VII collagen gene, COL7A1. Although revertant mosaicism is well known in DEB, 'forward' somatic mosaicism, in which a pathogenic mutation arises on a wild-type (WT) background, extending beyond

  14. Surface morphology changes and deuterium retention in Toughened, Fine-grained Recrystallized Tungsten under high-flux irradiation conditions

    Energy Technology Data Exchange (ETDEWEB)

    Oya, M., E-mail: ohya@st.eie.eng.osaka-u.ac.jp [Graduate School of Engineering, Osaka University, Suita, Osaka 565-0871 (Japan); Lee, H.T.; Ueda, Y. [Graduate School of Engineering, Osaka University, Suita, Osaka 565-0871 (Japan); Kurishita, H. [Institute for Materials Research, Tohoku University, Oarai, Ibaraki 311-1313 (Japan); Oyaidzu, M.; Hayashi, T. [Fusion Research and Development Directorate, International Fusion Energy Research Center, Japan Atomic Energy Agency, Rokkasho-mura, Aomori 039-3212 (Japan); Yoshida, N. [Research Institute for Applied Mechanics, Kyushu University, Fukuoka (Japan); Morgan, T.W.; De Temmerman, G. [FOM Institute DIFFER Dutch Institute for Fundamental Energy Research, Nieuwegein 3439 MN (Netherlands)

    2015-08-15

    Surface morphology changes and deuterium (D) retention in Toughened, Fine-Grained Recrystallized Tungsten (TFGR W) with TaC dispersoids (W-TaC) and pure tungsten exposed to D plasmas to a fluence of 10{sup 26} D/m{sup 2} s were studied as a function of the D ion flux (10{sup 22}–10{sup 24} D/m{sup 2} s). As the flux increased from 10{sup 22} D/m{sup 2} s to 10{sup 24} D/m{sup 2} s, the numbers of blisters increased for both materials. However, smaller blisters were observed on W-TaC compared to pure W. In W-TaC, cracks beneath the surface along grain boundaries were observed, which were comparable to the blister sizes. The reason for the smaller blister sizes may arise from smaller grain sizes of W-TaC. In addition, reduction of the D retention in W-TaC was observed for higher flux exposures. D depth profiles indicate this reduction arises due to decrease in trapping in the bulk.

  15. Herlitz junctional epidermolysis bullosa : diagnostic features, mutational profile, incidence and population carrier frequency in the Netherlands

    NARCIS (Netherlands)

    Yuen, W. Y.; Lemmink, H. H.; van Dijk-Bos, K. K.; Sinke, R. J.; Jonkman, M. F.

    2011-01-01

    Background Junctional epidermolysis bullosa, type Herlitz (JEB-H) is a lethal, autosomal recessive blistering disease caused by null mutations in the genes coding for the lamina lucida/densa adhesion protein laminin-332 (LAMB3, LAMA3 and LAMC2). Objectives To present the diagnostic features and mole

  16. Cantharidin toxicosis in 2 alpacas

    OpenAIRE

    Simpson, Katharine M.; Streeter, Robert N.; de Souza, Patricia; Genova, Suzanne G.; Morgan, Sandra E.

    2013-01-01

    Two adult alpacas were presented for recumbency and reluctance to rise. Cantharidin toxicosis was suspected based on clinical and ancillary diagnostic findings. The diagnosis was confirmed by gas chromatography-mass spectrometry of gastric contents and urine. Despite medical treatment, neither alpaca survived. Blister beetle toxicosis has not been previously described in camelids. Challenges in treatment of affected ruminants or pseudoruminants are noted.

  17. Manzinellaforgiftning kompliceret med streptokokpharyngitis og impetigo

    DEFF Research Database (Denmark)

    Bygbjerg, Ib Christian; Johansen, H K

    1991-01-01

    A Danish tourist to Antigua in the West Indies, ate a fruit of Hippomane mancinella and developed acute poisoning with blistering of the oral mucosa and diarrhoea and a streptococcal superinfection. Ignorant tourists and their travel advisers are warned against this potentially deadly apple-tree......-like plant and its tempting fruits....

  18. Phylogeny of an Albugo sp.> infecting Barbarea vulgaris in Denmark and its frequency of symptom development in natural populations of two evolutionary divergent plant types

    DEFF Research Database (Denmark)

    van Mölken, Tamara; Heimes, Christine; Hauser, Thure Pavlo;

    2014-01-01

    The oomycete Albugo candida has long been considered a broad spectrum generalist pathogen, but recent studies suggest that it is diverged into several more specialised species in addition to the generalist Albugo candida s.s.. Whereas these species cause the disease white blister rust in many cru...

  19. The Higgs Boson: Is the End in Sight?

    Science.gov (United States)

    Lincoln, Don

    2012-01-01

    This summer, perhaps while you were lounging around the pool in the blistering heat, the blogosphere was buzzing about data taken at the Large Hadron Collider at CERN. The buzz reached a crescendo in the first week of July when both Fermilab and CERN announced the results of their searches for the Higgs boson. Hard data confronted a theory nearly…

  20. Disease: H00844 [KEGG MEDICUS

    Lifescience Database Archive (English)

    Full Text Available The clinical features are uncomfortable skin blisters and vegetative lesions caused by friction. Mutations i...onifas JM, Beech J, Bench G, Shigihara T, Ogawa H, Ikeda S, Mauro T, Epstein EH Jr Mutations in ATP2C1, encoding a calcium pump, cause Hailey-Hailey disease. Nat Genet 24:61-5 (2000) ...

  1. Staying Up in a Down Economy

    Science.gov (United States)

    Pulley, John

    2009-01-01

    The fundraising landscape has been blistered by stock-market meltdowns and a financial sector that has repeatedly erupted in flames. In turn, the environment for development has turned icy, cooled by frozen credit markets and chilly consumer confidence. It is ragged, unstable terrain. Fundraisers, the ground moving beneath them, are unsure of…

  2. Paraneoplastische pemfigus bij een patiënt met een non-hodgkinlymfoom

    NARCIS (Netherlands)

    Wind, Lynnda J N; van der Velden, Annette W G; Diercks, Gilles F H; Pas, Hendrikus; Jonkman, Marcel F

    2010-01-01

    A 53-year-old man with non-Hodgkin lymphoma developed red, flaky skin, which was initially suggestive of a drug reaction. He also had pneumonia, for which he was admitted for antibiotic treatment. During admission the skin picture changed and blisters and erosions appeared on his body. Skin biopsy a

  3. 21 CFR 558.415 - Novobiocin.

    Science.gov (United States)

    2010-04-01

    ... lb. body weight per day. (a) Indications for use. Aid in the treatment of breast blisters associated... chickens; feed 5 to 7 days; withdraw 4 days before slaughter. (ii) Amount. Novobiocin, 10-14 mgs. per lb. body weight per day. (a) Indications for use. Treatment of staphylococcal synovitis and...

  4. Follicular non-Hodgkin's lymphoma with refractory paraneoplastic pemphigus : Case report with review of novel treatment modalities

    NARCIS (Netherlands)

    Van Rossum, MM; Verhaegen, NTM; Jonkman, MF; Mackenzie, MA; Koster, A; Van der Valk, PGM; Span, LFR

    2004-01-01

    In this paper a patient with a non-Hodgkin's lymphoma (NHL) and paraneoplastic pemphigus (PNP) is described. PNP is a very rare, painful mucocutaneous intraepithelial blistering disease associated with occult or confirmed malignancy. Patients with PNP show severe, progressive mucocutaneous disease w

  5. Follicular non-Hodgkin's lymphoma with refractory paraneoplastic pemphigus: case report with review of novel treatment modalities.

    NARCIS (Netherlands)

    Rossum, M.M. van; Verhaegen, N.T.; Jonkman, M.F.; MacKenzie, M.A.; Koster, A.; Valk, P.G.M. van der; Span, L.

    2004-01-01

    In this paper a patient with a non-Hodgkin's lymphoma (NHL) and paraneoplastic pemphigus (PNP) is described. PNP is a very rare, painful mucocutaneous intraepithelial blistering disease associated with occult or confirmed malignancy. Patients with PNP show severe, progressive mucocutaneous disease w

  6. Diabetic Foot

    Science.gov (United States)

    ... can cause you to lose feeling in your feet. You may not feel a cut, a blister or a sore. Foot injuries such as these can cause ulcers and ... the blood vessels can also mean that your feet do not get enough blood and oxygen. It ...

  7. Non-classical forms of pemphigus : pemphigus herpetiformis, IgA pemphigus, paraneoplastic pemphigus and IgG/IgA pemphigus

    NARCIS (Netherlands)

    Porro, Adriana Maria; Nasser Caetano, Livia de Vasconcelos; Nogueira Maehara, Laura de Sena; dos Santos Enokihara, Milvia Maria

    2014-01-01

    The pemphigus group comprises the autoimmune intraepidermal blistering diseases classically divided into two major types: pemphigus vulgaris and pemphigus foliaceous. Pemphigus herpetiformis, IgA pemphigus, paraneoplastic pemphigus and IgG/IgA pemphigus are rarer forms that present some clinical, hi

  8. The EGF repeat-specific O-GlcNAc-transferase Eogt interacts with notch signaling and pyrimidine metabolism pathways in Drosophila.

    Directory of Open Access Journals (Sweden)

    Reto Müller

    Full Text Available The O-GlcNAc transferase Eogt modifies EGF repeats in proteins that transit the secretory pathway, including Dumpy and Notch. In this paper, we show that the Notch ligands Delta and Serrate are also substrates of Eogt, that mutation of a putative UDP-GlcNAc binding DXD motif greatly reduces enzyme activity, and that Eogt and the cytoplasmic O-GlcNAc transferase Ogt have distinct substrates in Drosophila larvae. Loss of Eogt is larval lethal and disrupts Dumpy functions, but does not obviously perturb Notch signaling. To identify novel genetic interactions with eogt, we investigated dominant modification of wing blister formation caused by knock-down of eogt. Unexpectedly, heterozygosity for several members of the canonical Notch signaling pathway suppressed wing blister formation. And importantly, extensive genetic interactions with mutants in pyrimidine metabolism were identified. Removal of pyrimidine synthesis alleles suppressed wing blister formation, while removal of uracil catabolism alleles was synthetic lethal with eogt knock-down. Therefore, Eogt may regulate protein functions by O-GlcNAc modification of their EGF repeats, and cellular metabolism by affecting pyrimidine synthesis and catabolism. We propose that eogt knock-down in the wing leads to metabolic and signaling perturbations that increase cytosolic uracil levels, thereby causing wing blister formation.

  9. Caloplaca alstrupii, a new lichen species from Denmark

    DEFF Research Database (Denmark)

    Søchting, Ulrik

    1999-01-01

    Caloplaca alstrupii Søchting is described from an old Platanus in Denmark. The sorediate species has a greyish smooth thallus with very characteristic globose to subglobose blister-like areoles that rupture at maturity exposing pale yellowish green soredia. Apothecia sparse, orange, differing from...... those of the other species having soralia without anthraquinones....

  10. Herpetic Whitlow

    Science.gov (United States)

    ... the hibernating (latent) virus to wake up, become active, and travel back to the skin. These recurrent herpes simplex ... the prodrome phase (burning, tingling, or itching) and active phase (presence of blisters or ... gloves if you are a health care provider Don't pop any blisters—it ...

  11. The main problems of parents of a child with epidermolysis bullosa

    NARCIS (Netherlands)

    van Scheppingen, Corinne; Lettinga, Ant T.; Duipmans, Jose C.; Maathuis, Karel G. B.; Jonkman, Marcel F.

    2008-01-01

    Epidermolysis Bullosa (EB) is a rare genetic blistering- skin disorder with varying degrees of severity, ranging from mild forms to severe forms, with chronic progression. The aim of this study was to identify and specify the problems of parents of a child with EB. Qualitative research methodology w

  12. 49 CFR 571.116 - Standard No. 116; Motor vehicle brake fluids.

    Science.gov (United States)

    2010-10-01

    ..., multipurpose passenger vehicles, trucks, buses, trailers, and motorcycles. S4. Definitions. Blister means a..., multipurpose passenger vehicle, truck, bus, trailer, and motorcycle that has a hydraulic brake system shall be... heating mantle designed to fit the flask, or an electric heater with rheostat heat control....

  13. Restrictions in oral functions caused by oral manifestations of epidermolysis bullosa

    NARCIS (Netherlands)

    Stellingsma, Cornelis; Dijkstra, Pieter; Dijkstra, Janke; Duipmans, Jose C.; Jonkman, Marcel F.; Dekker, Rienk

    2011-01-01

    Several forms of epidermolysis bullosa (EB) present oral manifestations. Blistering of the (peri) oral mucosa affects the opening of the mouth, the mobility of the tongue and lips, thereby restricting oral functions. We describe the prevalence and characteristics of oral manifestations of EB in rela

  14. Damage phenomena of thin hard coatings submitted to repeated impacts: Influence of the substrate and film properties

    Energy Technology Data Exchange (ETDEWEB)

    Lamri, S. [IRTES-LERMPS, EA 7274, UTBM, 90 010 Belfort Cedex (France); ICD-LASMIS, UTT antenne de Nogent, Rue Lavoisier, 52800 Nogent (France); Langlade, C., E-mail: cecile.langlade@utbm.fr [IRTES-LERMPS, EA 7274, UTBM, 90 010 Belfort Cedex (France); Kermouche, G. [LTDS Laboratory, UMR CNRS 5513, ENI St Etienne (France); UMR CNRS 5146, Ecole des Mines de St-Etienne, Centre SMS, 158 Cours Fauriel, 42100 St-Etienne (France)

    2013-01-10

    To evaluate the surface fatigue resistance of some thin nitride films obtained by Physical Vapour Deposition (PVD) techniques, repeated impact tests have been performed under controlled impact conditions. Short and long duration tests have revealed the occurrence of an original damage phenomenon likely linked to a mechanical blistering of the films. As these blisters appear to be the first damage step, their formation has to be understood in order to be avoided in industrial applications. In particular, the role of the mechanical properties of the substrate has to be clarified as thin protective coatings may be used on pieces prepared using various heat treatments. finite element method (FEM) analysis has been conducted in order to better understand the specific mechanical conditions in the substrate and at the film-substrate interface that could lead to such blistering phenomena. Correlations with the experimental results have been evidenced. From the modelling results the substrate properties have been shown to be of significant influence on the blister formation. However as they do not fully explain the origin of this phenomenon, the influence of the substrate microstructure has also been studied and the presence of vanadium carbides appears to be of major effect.

  15. Effect of preemptive nerve block on inflammation and hyperalgesia after human thermal injury

    DEFF Research Database (Denmark)

    Pedersen, J L; Crawford, M E; Dahl, J B;

    1996-01-01

    whether a prolonged nerve block administered before a superficial burn injury could reduce local inflammation and late hyperalgesia after recovery from the block. METHODS: The effects of a preemptive saphenous nerve block on primary and secondary hyperalgesia, skin erythema, and blister formation, were...

  16. Interaction of implanted deuterium and helium with beryllium: radiation enhanced oxidation

    Energy Technology Data Exchange (ETDEWEB)

    Langley, R.A.

    1979-01-01

    The interaction of implanted deuterium and helium with beryllium is of significant interest in the application of first wall coatings and other components of fusion reactors. Electropolished polycrystalline beryllium was first implanted with an Xe backscatter marker at 1.98 MeV followed by either implantation with 5 keV diatomic deuterium or helium. A 2.0 MeV He beam was used to analyze for impurity buildup; namely oxygen. The oxide layer thickness was found to increase linearly with increasing implant fluence. A 2.5 MeV H/sup +/ beam was used to depth profile the D and He by ion backscattering. In addition the retention of the implant was measured as a function of the implant fluence. The mean depth of the implant was found to agree with theoretical range calculations. Scanning electron microscopy was used to observe blister formation. No blisters were observed for implanted D but for implanted He blisters occurred at approx. 1.75 x 10/sup 17/ He cm/sup -2/. The blister diameter increased with increasing implant fluence from about 0.8 ..mu..m at 10/sup 18/ He cm/sup -2/ to 5.5 ..mu..m at 3 x 10/sup 18/ He cm/sup -2/.

  17. Erythema elevatum diutinum in association with dermatitis herpetiformis

    Directory of Open Access Journals (Sweden)

    Shanmuga Sekar Chandrasekaran

    2014-01-01

    Full Text Available Erythema elevatum diutinum (EED is a rare skin disease that initially presents as leucocytoclastic vasculitis and later resolves with fibrosis. Dermatitis herpetiformis is an autoimmune blistering disease characterized by granular deposits of immunoglobulin A (IgA in dermal papillae. We report a rare association of these two disorders.

  18. Origin of microplasma instabilities during DC operation of silicon based microhollow cathode devices

    Science.gov (United States)

    Felix, Valentin; Lefaucheux, Philippe; Aubry, Olivier; Golda, Judith; Schulz-von der Gathen, Volker; Overzet, Lawrence J.; Dussart, Rémi

    2016-04-01

    The failure mechanisms of micro hollow cathode discharges (MHCD) in silicon have been investigated using their I-V characteristics, high speed photography and scanning electron microscopy. Experiments were carried out in helium. We observed I-V instabilities in the form of rapid voltage decreases associated with current spikes. The current spikes can reach values more than 100 times greater than the average MHCD current. (The peaks can be more than 1 Ampere for a few 10’s of nanoseconds.) These current spikes are correlated in time with 3-10 μm diameter optical flashes that occur inside the cavities. The SEM characterizations indicated that blister-like structures form on the Si surface during plasma operation. Thin Si layers detach from the surface in localized regions. We theorize that shallow helium implantation occurs and forms the ‘blisters’ whenever the Si is biased as the cathode. These blisters ‘explode’ when the helium pressure inside them becomes too large leading to the transient micro-arcs seen in both the optical emission and the I-V characteristics. We noted that blisters were never found on the metal counter electrode, even when it was biased as the cathode (and the Si as the anode). This observation led to a few suggestions for delaying the failure of Si MHCDs. One may coat the Si cathode (cavities) with blister resistant material; design the MHCD array to operate with the Si as the anode rather than as the cathode; or use a gas additive to prevent surface damage. Regarding the latter, tests using SF6 as the gas additive successfully prevented blister formation through rapid etching. The result was an enhanced MHCD lifetime.

  19. Clean, cold, and liquid-free laser transfer of biomaterials

    Science.gov (United States)

    Kononenko, T. V.; Nagovitsyn, I. A.; Chudinova, G. K.; Mihailescu, I. N.

    2011-04-01

    Blister-based laser induced forward transfer (BB-LIFT) is proposed as a promising tool for clean, cold and liquid-free local transfer of various organic substances. The feature of the given technique is that ejection of the material from the target results from non-destructive blistering of a thin metal film covering a transparent support. Applicability of the BB-LIFT driven by nanosecond laser pulses for micro-patterning of few organic Langmuir films was examined. Clean laser transfer with negligible material heating has been demonstrated for the nanoaggregated porphyrin films under optimized processing conditions. However, laser transfer of biopolymers, which form elastic and durable films at the target, meets essential problems and requires new solutions.

  20. Emergency management of chemical weapons injuries.

    Science.gov (United States)

    Anderson, Peter D

    2012-02-01

    The potential for chemical weapons to be used in terrorism is a real possibility. Classes of chemical weapons include nerve agents, vesicants (blister agents), choking agents, incapacitating agents, riot control agents, blood agents, and toxic industrial chemicals. The nerve agents work by blocking the actions of acetylcholinesterase leading to a cholinergic syndrome. Nerve agents include sarin, tabun, VX, cyclosarin, and soman. The vesicants include sulfur mustard and lewisite. The vesicants produce blisters and also damage the upper airways. Choking agents include phosgene and chlorine gas. Choking agents cause pulmonary edema. Incapacitating agents include fentanyl and its derivatives and adamsite. Riot control agents include Mace and pepper spray. Blood agents include cyanide. The mechanism of toxicity for cyanide is blocking oxidative phosphorylation. Toxic industrial chemicals include agents such as formaldehyde, hydrofluoric acid, and ammonia. PMID:22080590

  1. Degradation process in organic thin film devices fabricated using P3HT

    Indian Academy of Sciences (India)

    Rashmi; Ashok K Kapoor; Upendra Kumar; V R Balakrishnan; P K Basu

    2007-03-01

    The stability of regioregular poly(3-hexylthiophene 2,5-diyl) (P3HT) thin films sandwiched between indium tin oxide (ITO) and aluminium (Al) electrodes have been investigated under normal environmental conditions (25°C and RH ∼ 45-50%). Electrical and optical properties of ITO/P3HT/Al devices have been studied over a period of 30 days. Mobility of the order of 10-4 cm2/V-s has been obtained from the 2 law in the as- deposited P3HT films. Scanning electron microscopy (SEM) investigations show blistering of Al contacts in devices with a poly(3,4-ethylenedioxythiophene) (PEDOT) interlayer on application of voltage whereas no blistering is seen in devices without PEDOT. The results have been explained in terms of trap generation and propagation and the moisture-absorbing nature of PEDOT.

  2. Aphanius sophiae (Actinoptrygii, Cyprinodontidae), a new host for Ichthyophthirius multifiliis (Ciliophora) reported from Iran.

    Science.gov (United States)

    Gholami, Zeinab; Youssefi, Mohammad Reza; Marhaba, Zahra; Alizadeh, Abbas; Rahimi, Mohammad Taghi

    2016-09-01

    Aphanius sophiae (Heckel, 1849) or soffia tooth-carp is endemic species to the endorheic Kor River basin, Fars Province, Southwest of Iran. In spite of importance of this species as a biologic control of anopheles larvae, little attention has been paid to its diseases particularly parasitic infections. A total of 24 A. Sophiae (12 males and 12 females) in Safashahr spring, Kor River basin, were collected by using dip net in February 2012. Surface of all fish were gross examined and screened for any swelling and blisters. All of the examined specimens (100 %) were infected with Ichthyophthirius multifiliis. Infected fish typically developed small blister-like raised lesions on the body surface. The current study presents infection of A. sophiae with I. multifiliis for the first time from Iran. Hence, owing to considerable unexplored area of Iran further studies are required to increase our knowledge about parasitic infections of this fish. PMID:27605832

  3. Bullous pemphigoid after allogeneic hematopoietic stem cell transplantation.

    Science.gov (United States)

    Kato, Keisuke; Koike, Kazutoshi; Kobayashi, Chie; Iijima, Shigeruko; Hashimoto, Takashi; Tsuchida, Masahiro

    2015-06-01

    Bullous pemphigoid (BP) is an autoimmune skin disorder characterized by subepidermal blisters due to deposit of autoantibody against dermal basement membrane protein. It has been reported that BP can occur after allogeneic hematopoietic stem cell transplantation (HSCT). We describe a patient with BP having autoantibody against BP180 after unrelated-donor HSCT against T lymphoblastic leukemia. The patient was treated with steroid leading to complete resolution of BP, but T lymphoblastic leukemia progressed rapidly after steroid hormone treatment. Given that immunosuppressant may reduce graft-versus-tumor effect, immunomodulatory agents such as nicotinamide and tetracycline, erythromycin, and immunoglobulin may be appropriate as soon as typical blister lesions are seen after HSCT. PMID:26113316

  4. Meshed split skin graft for extensive vitiligo

    Directory of Open Access Journals (Sweden)

    Srinivas C

    2004-05-01

    Full Text Available A 30 year old female presented with generalized stable vitiligo involving large areas of the body. Since large areas were to be treated it was decided to do meshed split skin graft. A phototoxic blister over recipient site was induced by applying 8 MOP solution followed by exposure to UVA. The split skin graft was harvested from donor area by Padgett dermatome which was meshed by an ampligreffe to increase the size of the graft by 4 times. Significant pigmentation of the depigmented skin was seen after 5 months. This procedure helps to cover large recipient areas, when pigmented donor skin is limited with minimal risk of scarring. Phototoxic blister enables easy separation of epidermis thus saving time required for dermabrasion from recipient site.

  5. Novel compound heterozygous mutation in LAMC2 genes (c.79G>A and 382insT) in Herlitz junctional epidermolysis bullosa.

    Science.gov (United States)

    Jeon, In Kyung; Kim, Song-Ee; Kim, Soo-Chan

    2014-04-01

    Junctional epidermolysis bullosa (JEB) is a heritable blistering skin disease characterized by separation within the lamina lucida. It is caused by mutations in the LAMA3, LAMB3 and LAMC2 genes encoding the α3-, β3- and γ2-chains, respectively, of laminin-332. JEB Herlitz type (JEB-H) is a lethal blistering disease with severe cutaneous and extracutaneous involvements caused by null mutations in the gene encoding laminin-332. Here, we report a proband with JEB-H who is a compound heterozygote for two novel mutations in LAMC2; a missense mutation (c.79G>A) and an insertion mutation (382insT) leading to a premature termination codon. PMID:24533970

  6. Epidermolysis bullosa acquisita: current diagnosis and therapy

    Directory of Open Access Journals (Sweden)

    Christine R. Mehren

    2011-11-01

    Full Text Available Epidermolysis bullosa acquisita (EBA is an acquired, autoimmune subepidermal blistering disease with an approximate prevalence of 0,2/million people. The hallmark of EBA is the presence of autoantibodies (mainly IgG class to anchoring fibril collagen (type VII collagen located at the dermal-epidermal junction. Clinically EBA is subdivided into the inflammatory and the non-inflammatory phenotypes, depending on the level of the cleavage in the basal membrane. A recent addition to the diagnostic techniques is the analysis of the serration pattern of the autoantibody deposits at the basal membrane in the direct immunofluorescence. EBA and the closely related bullous systemic lupus erythematosus are the only diseases presenting with the so-called u-serration pattern which distinguishes them from many other autoimmune subepidermal blistering diseases. We also discuss the recent advances in therapy, including the experience with Rituximab.

  7. Micro- and nano-scale damage on the surface of W divertor component during exposure to high heat flux loads with He

    Science.gov (United States)

    Li, C.; Greuner, H.; Zhao, S. X.; Böswirth, B.; Luo, G. N.; Zhou, X.; Jia, Y. Z.; Liu, X.; Liu, W.

    2015-11-01

    Micro- and nano-scale surface damage on a W divertor component sample exposed to high heat flux loads generated with He atoms has been investigated through SEM, EBSD, AFM and FIB-SEM. The component sample was supplied by the Institute of Plasma Physics, Chinese Academy of Sciences (ASIPP) and AT&M company, China, and the loading experiment was performed in the GLADIS facility at IPP Garching, Germany. Two typical damage structures were observed on the surface: the first one is characterized by obvious blisters and some grooves formed from ruptured blisters, and the other one is a kind of porous structure accompanying with at least ∼25 nm surface material loss. As the grain orientation is further away from , the damage morphology gradually changes from the former structure to the latter. The possible damage mechanism is discussed.

  8. Bullosis Diabeticorum

    Directory of Open Access Journals (Sweden)

    Meina J. Michael, BS

    2016-03-01

    Full Text Available CASE A 63-year-old female with insulin-dependent type II diabetes mellitus and end-stage renal disease presented to the emergency department with spontaneous blistering to the tips of her left index and middle fingers. The blisters had gradually become tense and mildly painful over the preceding 10 days. She denied burn injury, trauma, fever, or new medications. On physical exam, the patient was noted to have a tense, nontender bullae on the pad of the left middle finger, and a collapsed, hemorrhagic bullae on the left index finger. There were no signs of inflammation or infection. A radiograph of the left hand, complete blood count, and basic metabolic panel were unremarkable. The diagnosis of bullosis diabeticorum was made, and supported by a consulting endocrinologist.

  9. In situ study of the initiation of hydrogen bubbles at the aluminium metal/oxide interface

    Science.gov (United States)

    Xie, De-Gang; Wang, Zhang-Jie; Sun, Jun; Li, Ju; Ma, Evan; Shan, Zhi-Wei

    2015-09-01

    The presence of excess hydrogen at the interface between a metal substrate and a protective oxide can cause blistering and spallation of the scale. However, it remains unclear how nanoscale bubbles manage to reach the critical size in the first place. Here, we perform in situ environmental transmission electron microscopy experiments of the aluminium metal/oxide interface under hydrogen exposure. It is found that once the interface is weakened by hydrogen segregation, surface diffusion of Al atoms initiates the formation of faceted cavities on the metal side, driven by Wulff reconstruction. The morphology and growth rate of these cavities are highly sensitive to the crystallographic orientation of the aluminium substrate. Once the cavities grow to a critical size, the internal gas pressure can become great enough to blister the oxide layer. Our findings have implications for understanding hydrogen damage of interfaces.

  10. Vitiligo after Diphencyprone for Alopecia Areata

    OpenAIRE

    Flávia Romero Gatti; Montealegre, F; João Mauricio Martins; Mario Cezar Pires

    2010-01-01

    The topical immunotherapy is used to treat alopecia areata and recalcitrant warts since the 1970s. Diphencyprone is a contact sensitizer used to treat dermatological conditions resulting from as altered immunological state, such as extensive alopecia areata, being partially effective and safe. Side effects include local eczema with blistering, regional lymphadenopathy and contact urticaria. Rare adverse effects include an erythema multiforme-like reaction, hyperpigmenttion, hypopigmentation, ...

  11. Three-year observation of the patients with bullous pemphigoid: 29 cases

    OpenAIRE

    Özlem Ekiz; Bilge Bülbül Şen; Emine Nur Rifaioğlu; Tuğba Şen; Esin Atik; Asena Çiğdem Doğramacı

    2013-01-01

    Background and Design: Bullous pemphigoid (BP) is a chronic, autoimmune, subepidermal blistering disease. The aim of this study was to determine the clinico-epidemiologic data’s of patients diagnosed as BP in our clinic and compare these data with other studies in our country and in the world. Material and Method: We researched the documents of BP cases diagnosed by clinical and histopathological examinations from January 2009 to January 2013, retrospectively. We evaluated clinical and de...

  12. Reproduction in laboratory of the morphology distribution and orientation of hydrides in different stages fuel cycle; Reproduccion en laboratorio de la morfologia, distribucion y orientacion de hidruros en distintas etapas del ciclo de combustible

    Energy Technology Data Exchange (ETDEWEB)

    Martin-Rengel, M. A.; Gomez, F. J.; Ruiz-Hervias, J.

    2013-07-01

    In this paper, the experimental techniques employed to reproduce in the laboratory the distribution, morphology and orientation of the hydrides during the different steps of the nuclear fuel cycle are reported. A cathodic charging technique was employed to produce ZIRLO cladding samples with an homogeneous distribution of hydrides and concentrations of 150, 250, 500, 1200 and 2000 ppm of hydrogen. The treatments developed to produce radial hydride reorientation, hydride blisters and a peripheral rim of hydrides are described.

  13. Linear IgA dermatosis adult variant with oral manifestation: A rare case report

    Directory of Open Access Journals (Sweden)

    T Isaac Joseph

    2015-01-01

    Full Text Available Linear immunoglobulin A (IgA dermatosis (LAD is a rare autoimmune disorder that presents as a vesiculo-bullous lesion with cutaneous manifestations, but rare oral mucosal involvement. Here we discuss a case of a vesiculobullous lesion with severe oral and ocular mucosal involvement mimicking pemphigoid with histopathological evidence of subepithelial blisters. Direct immunofluorescence (DIF confirmed the lesion as LAD of adult variant, although with atypical clinical features.

  14. Newborn with severe epidermolysis bullosa

    DEFF Research Database (Denmark)

    Boesen, Martin Lehmann; Bygum, Anette; Hertz, Jens Michael;

    2016-01-01

    Epidermolysis bullosa (EB) is an inherited skin disease with four main subtypes that cannot be distinguished clinically at birth. All subtypes may present with widespread life-threatening blisters and fragile skin, making treatment and handling of the newborn with EB challenging. The prognosis...... and nutrition. For immediate pain relief, intranasal fentanyl worked best and gabapentin was successfully used for chronic pain. The feeding difficulties were handled first by a nasogastric feeding tube. Later a normal feeding bottle proved to be adequate....

  15. The use of behavioral finance in the wealth management process

    OpenAIRE

    Opolski, Krzysztof; Potocki, Tomasz

    2011-01-01

    The economic theories taking into consideration human behavior and based on the achievements of psychology, sociology, anthropology have been evolving at a blistering pace over the last decade. Owing to that, the behavioral finance has become one of the fastest developing academic areas focused on the analysis of financial markets' behavior. The following presentation of the most common mistakes made by investors will allow the readers of this publication to develop more effective investment ...

  16. Application of Wiping Ointments Care in Treating Bullous Pemphigoid by Using Topical Steroid%擦药护理在外用激素治疗大疱性类天疱疮中的应用

    Institute of Scientific and Technical Information of China (English)

    张婷; 叶雅芬

    2012-01-01

    目的 探讨擦药护理在外用激素治疗大疱性类天疱疮中的效果.方法 按擦药操作流程给患者进行擦药,于用药前和用药1周后、2周后、3周后观察每天新发水疱数及全身水疱数.结果 用药3周后,患者每日新发水疱数及全身水疱数较用药前明显减少,差异有统计学意义(P<0.01).结论 擦药护理在外用激素治疗大疱性类天疱疮中起重要作用.%Objective To explore the effect of wiping ointments care for the treatment of bullous pemphig-oid by using topical steroid. Methods Patients were treated with wiping ointments according to wiping ointments operational process. Then the number of daily new blisters and total body blisters was recorded before treatment and 1 week,2 weeks,3 weeks after medication. Results The number of daily new blisters and total body blisters were significantly reducted after 3 weeks of treatment than that before treatment (P<0. 01). Conclusion Wiping Ointments Care plays an significant role for the treatment of bullous pem-phigoid by using topical steroid.

  17. Brassica collections for broadening agricultural use: RESGEN CT 99 109/112 Brassica rapa Subgroup.

    OpenAIRE

    Pinnegar, A.; Astley, D.; Dehmer, K.; Graichen, M. L.; Wilner, E.; Rosa, E; Aires, A.; Paula, R.; Ordás Pérez, Amando; Cartea González, María Elena

    2004-01-01

    The main tasks of the B. rapa subgroup in the project covered: production and preparation of data for the Bras-EDB; the development of a project core collection for B. rapa; characterisation of the core; regeneration of the core and other priority accessions in partner collections; evaluation of the core for i) resistance to White blister (Albugo candida}, b) glucosinolate content; safety duplication of material.

  18. Erythema Multiforme Following Application of Hair Dye

    OpenAIRE

    Sankha Koley; Jyotirindranath Sarkar; Sanjiv Choudhary; Suparna Dhara; Manoj Choudhury

    2012-01-01

    Erythema multiforme (EM) is an acute mucocutaneous hypersensitivity reaction with varying degrees of blistering and ulceration. Common causes of EM are herpes simplex virus infection, mycoplasma infection, drug hypersensitivity, vaccination and drug-virus interaction. EM induced by contact dermatitis is rare. Paraphenylene diamine, a common ingredient in many hair dyes, is well known to produce allergic contact dermatitis. We report a 35-year-old lady presenting with EM following severe conta...

  19. Dentistry Approach of Epidermolysis Bullosa: Two Case Reports

    OpenAIRE

    Filho, Gilberto Araujo Noro; Caputo, Bruno Vieira; de Carvalhosa, Artur Aburad; COSTA Claudio; Giovani, Elcio Magdalena

    2013-01-01

    Epidermolysis bullosa (EB) represents a group of genetically determined disorders characterized by development of blisters after minimal mechanical trauma or friction of the skin or mucosa with multiple oral manifestations. In the following we presented oral manifestations and therapy of two case reports with EB. Firstly we describe a 24 years-old caucasian female with diagnosis of epidermolysis bullosa, presence of vesiculobullous lesions on the hands and oral cavity. Secondly a 55 years-ol...

  20. Root and shoot growth of Pinus strobus x P. wallichiana somatic plants is influenced by the nitrogen composition of the germination medium

    OpenAIRE

    Llebres, M.T.; Pascual, M.B.; Cánovas, Francisco M.; Ávila, Concepción; Klimaszewska K

    2013-01-01

    Somatic embryogenesis (SE) in conifers has proven to be an useful biotechnological tool for species conservation and mass propagation, but to make it commercially viable, adaptive research is required to convert laboratory protocols into plantation reality. Hybrid white pines are of interest to forest industry in North America due to the potential resistance to white pine blister rust (Cronartium ribicola). Somatic embryos of many pine species are typically germinated on the sa...

  1. Growth, straightness and survival at age 32 in a Pinus strobus x P. wallichiana F1 hybrid population (Experiment 2)

    OpenAIRE

    Ioan Blada; Ştefan Tănasie

    2013-01-01

    By using an incomplete factorial mating design between twenty Pinus strobus L. female and seven P. wallichiana Jack. male trees, a number of 34 full-sib families were obtained. The objective of this experiment was to combine the fast growing of the former species with moderately to high resistance to blister rust (Cronartium ribicola Fich. in Rabenh.) of the latter one. The hybrid families were artificially inoculated at age two, and field planted at age four. The plantation took place within...

  2. Aberrant Expression and Secretion of Heat Shock Protein 90 in Patients with Bullous Pemphigoid

    OpenAIRE

    Stefan Tukaj; Konrad Kleszczyński; Katerina Vafia; Stephanie Groth; Damian Meyersburg; Piotr Trzonkowski; Ludwig, Ralf J; Detlef Zillikens; Enno Schmidt; Tobias W Fischer; Michael Kasperkiewicz

    2013-01-01

    The cell stress chaperone heat shock protein 90 (Hsp90) has been implicated in inflammatory responses and its inhibition has proven successful in different mouse models of autoimmune diseases, including epidermolysis bullosa acquisita. Here, we investigated expression levels and secretory responses of Hsp90 in patients with bullous pemphigoid (BP), the most common subepidermal autoimmune blistering skin disease. In comparison to healthy controls, the following observations were made: (i) Hsp9...

  3. Childhood Epidermolysis Bullosa Acquisita: Confirmation of Diagnosis by Skin Deficient in Type VII Collagen, Enzyme-linked Immunosorbent Assay, and Immunoblotting

    OpenAIRE

    Nupur Goyal; Raghavendra Rao; C. Balachandran; Sathish Pai; Bhogal, Balbir S.; Enno Schmidt; Detlef Zillikens

    2016-01-01

    Epidermolysis bullosa acquisita (EBA) is an acquired subepidermal bullous disorder characterized by autoantibodies against Type VII collagen. It usually affects adults; childhood EBA is rare. We describe a 10-year-old girl presenting with recurrent tense blisters predominantly on legs, dorsa of hands and feet accompanied by oral erosions since the age of 5 years. Direct immunofluorescence (IF) microscopy showed linear deposition of IgG and C3 along the basement membrane zone (BMZ); indirect I...

  4. A rare complication of follicular hair unit extraction: Kaposi’s varicelliform eruption

    OpenAIRE

    Mansur, A. Tulin; Demirci, Gulsen T.; Uzunismail, M. Adnan; Yildiz, Semsi

    2016-01-01

    Follicular hair unit extraction (FUE) is becoming a popular type of hair transplantation recently. Kaposi’s varicelliform eruption (KVE) is an uncommon skin emergency due to cutaneous dissemination of several types of viruses, most notably herpes virus, over the lesions of preexisting skin disorders. A 34-year-old man visited our dermatology outpatient clinic with a blistering, itchy and tender eruption on his head and body. He had undergone follicular FUE for androgenic alopecia 12 days prev...

  5. Oral vesiculobullous lesions: Consider the platelets

    Directory of Open Access Journals (Sweden)

    Clare Steel

    2014-01-01

    Full Text Available Oral vesiculobullous lesions or "blood blisters" can be found on a routine dental examination and may have many causes. Trauma is often the first diagnosis followed by a variety of bullous conditions, such as pemphigus and pemphigoid. Using a case report, we highlight the other, more serious, possibility of idiopathic thrombocytopenic purpura (ITP to raise awareness to the General Dental Practitioners and the need for prompt treatment.

  6. Early and Late Side Effects Associated with Photo(chemo)therapy

    OpenAIRE

    Günseli Öztürk

    2010-01-01

    Phototherapy (PUVA ve UVB) is a widely used and effective treatment method for a variety of dermatological diseases. Adverse effects associated with phototherapy can be classified as acute and chronic side effects. Acute side effects are mostly related with UV doses and drug intolerance, and include itching, nausea, erythema, edema and phototoxic reactions that sometimes blister formation is seen. Acute side effects are usually moderate and transient. Chronic side effects of phototherapy are ...

  7. Opatření v ohnisku výskytu slintavky a kulhavky a jeho okolí

    OpenAIRE

    MUSILOVÁ, Karolína

    2015-01-01

    This bachelor's thesis called "Control measures for an outbreak of foot and mouth disease at the point of origin and its surroundings" deals with problems concerning FMD disease occurrence in Chlebov livestock holding belonging to Reprogen JSC. FMD is extremely dangerous and highly contagious disease causing high fever that affects especially cloven-hoofed animals. A viral genus Aphthovirus is the originator, causing blisters and aphthae on intestinal tract, limbs and hairless skin, other sym...

  8. Detection of hand, foot and mouth disease in the Yucatan Peninsula of Mexico

    OpenAIRE

    Carlos Machain-Williams; Dzul-Rosado, Alma R.; Aarón B. Yeh-Gorocica; Rodriguez-Ruz, Katia G.; Henry Noh-Pech; Lourdes Talavera-Aguilar; Ma. Isabel Salazar; María Eugenia Castro-Mussot; Guadalupe Reyes-Solis; Julián E. Garcia-Rejon; Puerto-Manzano, Fernando I.; Blitvich, Bradley J.

    2014-01-01

    We report a case of hand, foot and mouth disease (HFMD) in a 5-year-old male from Merida City in the Yucatan Peninsula of Mexico. A clinical and physical examination revealed that the patient had symptoms typical of HFMD, including fever, fatigue, odynophagia, throat edema, hyperemia, lesions on the hands and feet, and blisters in the oral cavity. The patient fully recovered after a convalescence period of almost three weeks. Reverse transcription-polymerase chain reaction and nucleotide sequ...

  9. Hand, Foot, and Mouth Syndrome in an Immunocompetent Adult: a Case Report

    OpenAIRE

    Flor de Lima, B; Silva, J.; Rodrigues, AC; Grilo, A.; Riso, N; Vaz Riscado, M

    2013-01-01

    BACKGROUND: Hand, foot, and mouth syndrome (HFMS) is a common acute illness. It is characterized by mild clinical symptoms including fever, blisters, and sores in the mouth and on the palms and soles following a 3- to 7-day incubation period. This syndrome is rarely seen in adults. CASE PRESENTATION: A 35-year-old male Caucasian patient had a history of multiple episodes of acute pharyngitis, hypertension, hypercholesterolemia, and occasional abdominal pain. He presented with polyarthra...

  10. Echovirus 4 associated to hand, foot and mouth disease Echovirus 4 associado à doença de mão, pé e boca

    OpenAIRE

    Denise Hage Russo; Adriana Luchs; Bráulio Caetano Machado; Rita de Cássia Carmona; Maria do Carmo Sampaio Timenetsky

    2006-01-01

    Hand, foot and mouth disease (HFMD) is a contagious enteroviral infection occurring primarily in children and characterized by vesicular palmoplantar eruptions and erosive stomatitis. Echovirus 4 (EV-4) has been commonly associated with aseptic meningitis. The association of HFMD with EV-4 has not been reported previously. Two samples of a 14-month child who presented mild fever, sores in the mouth, rash with blisters on the palm of hands and soles of feet were sent to Enteric Viruses Laborat...

  11. A Case of Eczema Herpeticum with Hailey-Hailey Disease

    OpenAIRE

    Lee, Gun Hong; Kim, Yun Mi; Lee, Sung Yul; Lee, Jong Suk; Park, Young Lip; Whang, Kyu Uang

    2009-01-01

    Eczema herpeticum is the dissemination of herpes simplex virus in the setting of a preexisting skin disease. Hailey-Hailey disease [familial benign chronic pemphigus] is a blistering dermatosis that is inherited as an autosomal dominant trait and it usually presents itself around the third or fourth decades. Coexistence of eczema herpeticum and Hailey-Hailey disease is an infrequent occurrence. Four such cases have been reported in the English and German medical literature. We report here on ...

  12. Bullous Darier’s disease mimicking Hailey-Hailey disease

    OpenAIRE

    Khaled, Aida; Ben Hamida, Myriam; Goucha, Samia; Zermani, Rachida; Rameh, Soumaya; Ben Taazayet, Sabra; Kamoun, Mohamed Ridha; Fazaa, Bécima

    2011-01-01

    Background The bullous variant of Darier’s disease (DD) is a rare type characterized by histological and clinical overlap with Hailey-Hailey disease (HDD). Case reports The following case report describes two cases of familial DD; a 51-year-old woman who presented with erythematous plaques, covered by small blisters in axillary and inguinal areas, and the first patient’s daughter, who presented with keratotic papules localized on the axillary and inframammary folds. Conclusion These two cases...

  13. STUDY OF THE MOLECULAR ALTERATIONS AND TREATMENT OF HAILEY-HAILEY DISEASE

    OpenAIRE

    Manca, Sonia

    2014-01-01

    Hailey-Hailey Disease (HHD) is a chronic, recurrent blistering rare disorder, clinically characterized by erosions occurring primarily in intertriginous regions and histologically by suprabasal cutaneous cells separation (acantholysis). Despite a strong relationship between mutations in ATP2C1 gene, encoding for hSPCA1 calcium pump, manifestations of the disease occurs with high variability between patients. In our previous studies we used primary keratinocytes derived from les...

  14. Hailey-Hailey Disease Responding to Thalidomide

    OpenAIRE

    K Bala Nanda; Celia Soni Saldanha; Martis Jacintha; Ganesh Kamath

    2014-01-01

    Familial benign chronic pemphigus or Hailey-Hailey disease (HHD) is a rare autosomal dominant disorder characterized by the development of recurrent blisters and erosions in the intertriginous areas. Various topical and systemic treatment options include corticosteroids, topical 5-fluorouracil, topical vitamin D analogs, topical zinc oxide, dapsone, psoralen plus ultraviolet A, systemic retinoids, cyclosporine, methotrexate, and photodynamic therapy. In recalcitrant cases, further options inc...

  15. Identification of Several Mutations in ATP2C1 in Lebanese Families: Insight into the Pathogenesis of Hailey-Hailey Disease

    OpenAIRE

    Waed Btadini; Ossama K. Abou Hassan; Dana Saadeh; Ossama Abbas; Farah Ballout; Abdul-Ghani Kibbi; Ghassan Dbaibo; Nadine Darwiche; Georges Nemer; Mazen Kurban

    2015-01-01

    Background Hailey-Hailey disease (HHD) is an inherited blistering dermatosis characterized by recurrent erosions and erythematous plaques that generally manifest in intertriginous areas. Genetically, HHD is an autosomal dominant disease, resulting from heterozygous mutations in ATP2C1, which encodes a Ca2+/Mn2+ATPase. In this study, we aimed at identifying and analyzing mutations in five patients from unrelated families diagnosed with HHD and study the underlying molecular pathogenesis. Objec...

  16. Efficacy of the melanocortin analogue Nle4-D-Phe7-α-melanocyte-stimulating hormone in the treatment of patients with Hailey–Hailey disease

    OpenAIRE

    Biolcati, G; Aurizi, C; Barbieri, L; Cialfi, S; Screpanti, I; Talora, C

    2013-01-01

    Background Hailey–Hailey disease (HHD) is a rare, chronic and recurrent blistering disorder, which is characterized clinically by erosions occurring primarily in intertriginous regions, and histologically by suprabasal acantholysis. Oxidative stress plays a specific role in the pathogenesis of HHD, by regulating the expression of factors playing an important role in keratinocyte proliferation and differentiation. Aim Given the significance of oxidative stress in HHD, we investigated the poten...

  17. Combined Therapeutic Use of Oral Alitretinoin and Narrowband Ultraviolet-B Therapy in the Treatment of Hailey-Hailey Disease

    OpenAIRE

    Vanderbeck, Kaitlin A.; Lyne Giroux; Murugan, Nirosha J.; Karbowski, Lukasz M.

    2014-01-01

    Hailey-Hailey disease (HHD) is a chronic familial bullous disease characterized by recurrent blisters and erosions typically at friction-prone areas of the body accompanied by acantholysis upon histologic examination. There are a number of therapies used in the management of HHD. Its symptoms have been effectively treated with antimicrobial therapies, corticosteroids and other agents such as cyclosporine and prednisone. However, such treatments are not always effective. Therefore, there is a ...

  18. Sporadic porphyria cutanea tarda due to haemochromatosis

    OpenAIRE

    Geus, Hilde; Dees, A.

    2006-01-01

    textabstractHaemochromatosis is a hereditary iron-overload syndrome caused by increased intestinal iron absorption and characterised by accumulation of potentially toxic iron in the tissues. Sometimes this disease presents as a cutanea porphyria. We describe a patient with joint complaints and blistering skin lesions on sun-exposed skin. After identifying the porphyria cutanea tarda by urine analysis we found that the serum activity of uroporphyrinogen decarboxylase (UROD) was normal, meaning...

  19. Statistical modeling for correlate of protection using accelerated failure time models and piecewise methods.

    OpenAIRE

    Mogeni, Polycarp

    2013-01-01

    Chicken pox is an important childhood illness affecting mostly school-going children. The disease can be spread through contacts between infected and susceptible individuals. It is a very contagious disease caused by the varicella-zoster virus. Its main symptoms are: blister-like rash, tiredness, itching, and fever. Chicken pox can be serious, especially in adults, babies, and people with weakened immune systems. The objective of this study was to apply parametric survival models to determine...

  20. Staphylococcal Scalded-Skin Syndrome Complicating Wound Infection in a Preterm Infant with Postoperative Chylothorax

    Science.gov (United States)

    Peters, Bjoern; Hentschel, Juliane; Mau, Harald; Halle, Elke; Witte, Wolfgang; Obladen, Michael

    1998-01-01

    The course of infection in a 3-week-old premature newborn suffering from extensive dermatitis with flaccid blisters is described. Staphylococcus aureus was recovered from a local wound infection around a chest tube inserted to drain a postoperative chylothorax. The strain isolated tested positive for the eta gene for exfoliative toxin A, the causative agent of staphylococcal scalded-skin syndrome (SSSS). In this case, prematurity and loss of chylus with consecutive lymphopenia may have contributed to development of SSSS. PMID:9738069

  1. Nicotinamide and the skin.

    Science.gov (United States)

    Chen, Andrew C; Damian, Diona L

    2014-08-01

    Nicotinamide, an amide form of vitamin B3, boosts cellular energy and regulates poly-ADP-ribose-polymerase 1, an enzyme with important roles in DNA repair and the expression of inflammatory cytokines. Nicotinamide shows promise for the treatment of a wide range of dermatological conditions, including autoimmune blistering disorders, acne, rosacea, ageing skin and atopic dermatitis. In particular, recent studies have also shown it to be a potential agent for reducing actinic keratoses and preventing skin cancers.

  2. Characteristics of a low energy and high flux compact plasma source and preliminary results in studying surface modification of tungsten irradiated by the source

    International Nuclear Information System (INIS)

    A plasma source has been constructed in the Tritium Engineering Laboratory for investigating plasma-surface interactions in a tokamak like ITER. It is a compact device with a total length less than 1 m, compared to other existing facilities in the world. However, it can provide with stable plasma beams of low energy (∼100 eV) and high flux (∼1022/m2/s), close to the predicted edge plasma conditions near the ITER divertor. This report mainly describes its configuration and its characteristics, including influence of filament, arc discharge, magnetic field, bias voltage, and so on, on plasma beam being delivered towards the sample. Also shown are the results of preliminary experiments of blister formation on tungsten samples irradiated by a deuterium plasma beam generated with the source. Hot-rolled powder-metallurgy tungsten samples of 0.1 mm thick were irradiated at ambient temperature using the plasma beam with ion flux ranging from 2x1021 to 1x1022/m2/s, fluence from 4x1022 to 1x1026/m2, and incident energy around 100 eV, corresponding to the negative bias applied on the samples. Blisters were formed over a critical fluence around 1x1023/m2. Blister density increased firstly to its maximum of 3.5x104/mm2 at a fluence of about 2x1024/m2, and then decreased with increasing the fluence to its maximum in this work. In contrast, blister size increased monotonically up to 6 μm in diameter with the fluence till its maximum. The results confirmed the applicability of this plasma source in simulating the ITER divertor plasma. Using this plasma source and the thermal desorption spectroscopy under construction, study on the retention of hydrogen isotopes in the plasma facing components is planned. (author)

  3. A Rare Case of Pilomatricoma with Bullous Appearance

    OpenAIRE

    Karadağ, Ayşe Serap; EKŞİOĞLU, Meral; ÖZLÜK, Ekin; AKBAY, Gülfer

    2009-01-01

    Background: Pilomatricoma is a rare, benign adnexal tumor differentiating toward elements of the hair matrix and shaft. It typically presents as a solitary, deep, dermal nodule. In addition, bullous changes can be rarely seen. Methods: A 16-year-old girl with firm, mobile, multilobule and non-tender mass that has a soft, hemorrhagic blister-like formation on the left infraclavicular region is described. Results: The histopathologic findings were consistent with pilomatricoma. In the dermis...

  4. Kindler syndrome with palmoplantar hyperhidrosis and blonde hair

    Directory of Open Access Journals (Sweden)

    Anshul Maheshwari

    2015-01-01

    Full Text Available Kindler syndrome (KS is a very rare genodermatosis characterized by acral blistering starting in infancy along with photosensitivity, progressive poikiloderma, cutaneous atrophy, and a variable degree of mucosal involvement. A large number of other cutaneous and extracutaneous features have been described, which aid in diagnosing it. Generally KS has been found to be associated with hypohidrosis/anhidrosis. We herein present a rare case of KS with unique features.

  5. OA02.12. Poly herbal ayurvedic formulation for the development of anti–miliaria cotton fabrics

    OpenAIRE

    V.Rajalakshmi; Rajendran, R.; Radhai, R; ,

    2013-01-01

    Purpose: Miliaria is also known as Sweat rash or Prickly heat, common aliment of the eccrine sweat glands that often occurs in conditions of increased heat and humidity. It is especially prevalent in babies and infants. Miliaria crystalline is the one which cause multiple tiny blisters resulting in rapidly evolving noninflammatory vesicles. The present study was mainly carried out to explore the use of herbal plants primarily Neem (Azadirachta indica) and Mint (Mentha arvensis) leaves for the...

  6. Thermal cycling and high power density hydrogen ion beam irradiation of tungsten layers on tungsten substrate

    Science.gov (United States)

    Airapetov, A. A.; Begrambekov, L. B.; Gretskaya, I. Yu; Grunin, A. V.; Dyachenko, M. Yu; Puntakov, N. A.; Sadovskiy, Ya A.

    2016-09-01

    Tungsten layers with iron impurity were deposited on tungsten substrates modeling re-deposited layers in a fusion device. The samples were tested by thermocycling and hydrogen ion beam tests. Thermocycling revealed globule formation on the surface. The size of the globules depended on iron impurity content in the coating deposited. Pore formation was observed which in some cases lead to exfoliation of the coatings. Hydrogen ion irradiation lead to formation of blisters on the coating and finally its exfoliation.

  7. Automatic differentiation of u- and n-serrated patterns in direct immunofluorescence images

    OpenAIRE

    Shi, Chenyu; Guo, Jiapan; Azzopardi, George; Meijer, Joost; Jonkman, Marcel F.; Petkov, Nicolai

    2015-01-01

    Epidermolysis bullosa acquisita (EBA) is a subepidermal autoimmune blistering disease of the skin. Manual u- and n-serrated patterns analysis in direct immunofluorescence (DIF) images is used in medical practice to differentiate EBA from other forms of pemphigoid. The manual analysis of serration patterns in DIF images is very challenging, mainly due to noise and lack of training of the immunofluorescence (IF) microscopists. There are no automatic techniques to distinguish these two types of ...

  8. DUAL EPISODES OF HERPES ZOSTER IN A SINGLE YEAR IN IMMUNOCOMPETENT MALE CASE REPORT

    OpenAIRE

    Gaurav; Anil Kumar; Sumi

    2014-01-01

    Herpes zoster, is a viral disease characterised by painful skin rash with blister in a limited area on one side of body. Varicella zoster virus1 is the cause of both varicella (Chicken pox) and zoster (Shingles).It initially causes chicken pox and then remains latent for decades in cranial nerve, dorsal root and autonomic nervous system ganglia. 2 Three genotypes of this alpha – herpesvirus have been identified and shown to have geographical variations. 3 The virus gets ...

  9. Respiratory Effects of Amifostine and DRDE-07: Probable Prophylactic Agents of Sulphur Mustard in Rats

    OpenAIRE

    Seema Singh; Vimal Malviya; Anshoo Gautam; Ram Singh; Uma Pathak; Raza, S K; Vijayaraghavan, R.

    2006-01-01

    Amifostine (S-2[3-aminopropylamino]ethyl phosphorothioate) and one of its analogues,DRDE-07 (S-2[2-aminoethylamino]ethyl phenyl sulphide) are promising prophylactic agents forsulphur mustard (SM; a blistering agent) toxicity. When given orally, DRDE-07 was more effectivethan amifostine as a prophylactic agent against SM administered percutaneously. Variouspharmacological and toxicological studies are required before the introduction of a chemical asa drug. The respiratory effects of amifostin...

  10. Prophylactic Efficacy of Amifostine, DRDE-07, and their Analogues against Percutaneously Administered Nitrogen Mustards and Sulphur Mustard

    OpenAIRE

    Manoj Sharma; Vijayaraghavan, R.; Uma Pathak; K. Ganesan

    2009-01-01

    Nitrogen mustards (HN-1, HN-2 and HN-3) and sulphur mustard are alkylating and blister-inducing chemical warfare agents. This study was aimed at investigating the prophylactic efficacy of amifostine, DRDE-07, and their analogues and some recommended antidotes against dermally-applied nitrogen mustards and sulphur mustard in preventing their systemic toxicity in mice. The antidotes were administered as single oral dose, 30 min prior to the mustard agent application. For DRDE-07, 0.2 LD50 (249 ...

  11. Nicotinamide and the skin.

    Science.gov (United States)

    Chen, Andrew C; Damian, Diona L

    2014-08-01

    Nicotinamide, an amide form of vitamin B3, boosts cellular energy and regulates poly-ADP-ribose-polymerase 1, an enzyme with important roles in DNA repair and the expression of inflammatory cytokines. Nicotinamide shows promise for the treatment of a wide range of dermatological conditions, including autoimmune blistering disorders, acne, rosacea, ageing skin and atopic dermatitis. In particular, recent studies have also shown it to be a potential agent for reducing actinic keratoses and preventing skin cancers. PMID:24635573

  12. Opportunities in Pharmaceutical RFID and Smart Packaging

    Institute of Scientific and Technical Information of China (English)

    Dr; Peter; Harrop

    2006-01-01

    RFID in healthcare is growing rapidly to become a $2.1 billion global business in 2016. Smart packaging for healthcare has additional value, for example in patient compliance recording blister packs that electronically record when each pill is taken - or more strictly when it is removed from the pack. Aardex has a different version where the plastic bottle of pills is continuously weighed by a load cell in the base. Thus recording when a pill is removed. These pack-

  13. Identification of the cutaneous basement membrane zone antigen and isolation of antibody in linear immunoglobulin A bullous dermatosis.

    OpenAIRE

    Zone, J J; Taylor, T B; Kadunce, D P; Meyer, L J

    1990-01-01

    Linear IgA bullous dermatosis (LABD) is a rare blistering skin disease characterized by basement membrane zone deposition of IgA. This study identifies a tissue antigen detected by patient serum and then isolates the autoantibody using epidermis and protein bands blotted on nitrocellulose as immunoabsorbents. Sera from 10 patients (9 with cutaneous disease and 1 with cicatrizing conjunctivitis) were evaluated. Indirect immunofluorescence revealed an IgA anti-basement membrane antibody in 6 of...

  14. Evidences for liquid encapsulation in PMMA ultra-thin film grown by liquid injection Photo-CVD

    OpenAIRE

    Manole, Claudiu Constantin; Marsan, Olivier; Charvillat, Cédric; Demetrescu, Ioana; Maury, Francis

    2013-01-01

    This paper deals with the characterization of ultra-thin films of PMMA grown by an original photoassisted Chemical Vapor Deposition process equipped with a pulsed liquid injection system to deliver the monomer. The nanometric thick films showed a good ability to encapsulate a liquid phase as microdroplets protected by a thin polymeric tight membrane in the form of blisters. Techniques that are capable to analyze these heterogeneous structures at micro- and nanoscopic scale such as Raman Confo...

  15. Occurrence of epidermolysis bullosa along with Amelogenesis imperfecta in female patient of India

    Directory of Open Access Journals (Sweden)

    A P Javed

    2013-01-01

    Full Text Available Epidermolysis bullosa (EB is an inherited disorder, which is characteristically presented as skin blisters developing in response to minor injury. Junctional variety of EB is also associated with enamel hypoplasia. Amelogenesis imperfecta presents with abnormal formation of the enamel both in deciduous and permanent dentition. This article describes a previously unreported case of Amelogenesis imperfecta with complete loss of enamel in a young female patient with EB.

  16. Bioactivity of Rumex obtusifolius (Polygonaceae)

    OpenAIRE

    Harshaw Diane; Nahar Lutfun; Vadla Brahmachari; Saif-E-Naser Gadria M.; Sarker Satyajit D.

    2010-01-01

    Rumex obtusifolius L. (Polygonaceae), commonly known as 'broad-leaf dock', is one of the most common Irish wayside weeds, and it also occurs in silage fields, on river banks, in ditches and on waste grounds. The ethnobotanical uses of this species include its use as an antidote to nettle, depurative, astringent, laxative, and tonic, and in the treatment of sores, blisters, burns, cancer and tumors. The bioactivities of n-hexane, dichloromethane (DCM) and methanol (MeOH) extracts of the leaves...

  17. THE PRELUDE ON CONVENTIONAL AND ADVANCE TREATMENT OF PEMPHIGUS VULAGARIS (PV): A REVIEW

    OpenAIRE

    Basappa Karisetty; Venu Gopal Jonnalagadda

    2013-01-01

    The Pemphigus vulgaris (PV) is a group of highly lethal autoimmune disease characterized by mucocutaneous erosions or blisters. Pemphigus vulgaris can be present in different forms like oral and topical. So, it is challenging to manage and treat. Mortality rate due to Pemphigus vulgaris can be decreased by using systemic corticosteroids, but the present treatment options are limited due to the toxicity profiles of drugs available for treating Pemphigus vulgaris. Recent studies that shed light...

  18. Wound Healing of Cutaneous Sulfur Mustard Injuries

    OpenAIRE

    Graham, John S.; Chilcott, Robert P.; Rice, Paul; Milner, Stephen M.; Hurst, Charles G.; Maliner, Beverly I.

    2005-01-01

    Sulfur mustard is an alkylating chemical warfare agent that primarily affects the eyes, skin, and airways. Sulfur mustard injuries can take several months to heal, necessitate lengthy hospitalizations, and result in significant cosmetic and/or functional deficits. Historically, blister aspiration and/or deroofing (epidermal removal), physical debridement, irrigation, topical antibiotics, and sterile dressings have been the main courses of action in the medical management of cutaneous sulfur m...

  19. Engineering patterns of wrinkles and bubbles in supported graphene through modeling and simulation

    OpenAIRE

    Zhang, Kuang

    2015-01-01

    Graphene deposited on a substrate often exhibits out-of-plane deformations with different features and origins. Networks of localized wrinkles have been observed in graphene synthesized through CVD, as a result of compressive stresses transmitted by the substrate. Graphene blisters have been reported with various sizes and shapes, and have been shown to be caused by gas trapped between graphene and substrate. Such wrinkles or bubbles locally modify the electronic properties and are often seen...

  20. A Role for Mitochondrial Oxidative Stress in Sulfur Mustard Analog 2-Chloroethyl Ethyl Sulfide-Induced Lung Cell Injury and Antioxidant Protection

    OpenAIRE

    Gould, Neal S; White, Carl W; Day, Brian J.

    2008-01-01

    Sulfur mustards (SMs) have been used as warfare agents since World War I and still pose a significant threat against civilian and military personnel. SM exposure can cause significant blistering of the skin, respiratory injury, and fibrosis. No antidote currently exists for SM exposure, but recent studies, using the SM analog 2-chloroethyl ethyl sulfide (CEES), have focused on the ability of antioxidants to prevent toxicity. Although antioxidants can prevent CEES-induc...

  1. Acetaminophen-induced cellulitis-like fixed drug eruption

    OpenAIRE

    Neila Fathallah; Chaker Ben Salem; Raoudha Slim; Lobna Boussofara; Najet Ghariani; Kamel Bouraoui

    2011-01-01

    Acetaminophen is a widely used analgesic drug. Its adverse reactions are rare but severe. An 89-year-old man developed an indurated edematous and erythematous plaque on his left arm 1 day after acetaminophen ingestion. Cellulitis was suspected and antibiotictherapy was started but there was no improvement of the rash; there was a spectacular extension of the lesion with occurrence of flaccid vesicles and blisters in the affected sites. The diagnosis of generalized-bullous-fixed drug eruption ...

  2. Penetration of Moxifloxacin into Peripheral Compartments in Humans

    OpenAIRE

    Müller, Markus; Staß, Heino; Brunner, Martin; Möller, Jan G.; Lackner, Edith; Eichler, Hans G.

    1999-01-01

    To characterize the penetration of moxifloxacin (BAY 12-8039) into peripheral target sites, the present study aimed at measuring unbound moxifloxacin concentrations in the interstitial space fluid by means of microdialysis, an innovative clinical sampling technique. In addition, moxifloxacin concentrations were measured in cantharides-induced skin blisters, saliva, and capillary plasma and compared to total- and free-drug concentrations in venous plasma. For this purpose, 12 healthy volunteer...

  3. Assesing the acceptance of the project

    OpenAIRE

    Clara Fernández-Shaw Toda; David García Marco; Inmaculada Díaz Fernández

    2009-01-01

    OBJECTIVE: Evaluate the acceptability of the project "Adding colour to your unit dose." MATERIAL AND METHODS: It sets in motion a project to reduce errors in the administration of pasting labels with different shapes and colours on blister medicines. It includes the name of the product, the active ingredient, dosage, lot and expiration date. Also included for internal quality control are the initials of the person who made the label and the date and time at which it was made. The nurse monito...

  4. Epidemiology of pemphigus in northeast Iran: A 10-year retrospective study

    OpenAIRE

    Javidi Zari; Meibodi Naser; Nahidi Yalda

    2007-01-01

    Pemphigus is an autoimmune blistering disease of the skin and mucous membranes with different reported epidemiological aspects from different countries. Aim: Our aim was to evaluate the epidemiological aspects of pemphigus in Mashhad city. This is the first report with regard to the characteristics of pemphigus in Khorasan province of Iran. Materials and Methods: In this cross-sectional study, all the patients with pemphigus, who were referred to the Dermatology Department of Imam Reza ho...

  5. Research on the Interaction of Hydrogen-Bond Acidic Polymer Sensitive Sensor Materials with Chemical Warfare Agents Simulants by Inverse Gas Chromatography

    OpenAIRE

    Liu Yang; Qiang Han; Shuya Cao; Feng Huang; Molin Qin; Chenghai Guo; Mingyu Ding

    2015-01-01

    Hydrogen-bond acidic polymers are important high affinity materials sensitive to organophosphates in the chemical warfare agent sensor detection process. Interactions between the sensor sensitive materials and chemical warfare agent simulants were studied by inverse gas chromatography. Hydrogen bonded acidic polymers, i.e., BSP3, were prepared for micro-packed columns to examine the interaction. DMMP (a nerve gas simulant) and 2-CEES (a blister agent simulant) were used as probes. Chemical an...

  6. Shared VH1-46 gene usage by pemphigus vulgaris autoantibodies indicates common humoral immune responses among patients

    OpenAIRE

    Cho, Michael Jeffrey; Lo, Agnes S.Y.; Mao, Xuming; Nagler, Arielle R.; Christoph T Ellebrecht; Mukherjee, Eric M.; Hammers, Christoph M.; Choi, Eun-Jung; Sharma, Preety M.; Uduman, Mohamed; LI Hong; Rux, Ann H.; Farber, Sara A.; Rubin, Courtney B.; Kleinstein, Steven H

    2014-01-01

    Pemphigus vulgaris (PV) is a potentially fatal blistering disease caused by autoantibodies against desmoglein 3 (Dsg3). Here, we clone anti-Dsg3 antibodies from four PV patients and identify pathogenic VH1-46 autoantibodies from all four patients. Unexpectedly, VH1-46 autoantibodies had relatively few replacement mutations. We reverted antibody somatic mutations to their germline sequences to determine the requirement of mutations for autoreactivity. Three of five VH1-46 germline-reverted ant...

  7. Adherence to a Six-Dose Regimen of Artemether-Lumefantrine for Treatment of Uncomplicated Plasmodium Falciparum Malaria in Uganda.

    OpenAIRE

    Fogg, Carole; Bajunirwe, Francis; Piola, Patrice; Biraro, Samuel; Checchi, Francesco; Kiguli, James; Namiiro, Proscovia; Musabe, Joy; Kyomugisha, Agnes; Guthmann, Jean-Paul

    2004-01-01

    Measuring baseline levels of adherence and identifying risk factors for non-adherence are important steps before the introduction of new antimalarials. In Mbarara in southwestern Uganda, we assessed adherence to artemether-lumefantrine (Coartem) in its latest World Health Organization blister formulation. Patients with uncomplicated Plasmodium falciparum malaria were prescribed artemether-lumefantrine and received an explanation of how to take the following five doses at home. A tablet count ...

  8. Quality of Life and Economic Burden in Recessive Dystrophic Epidermolysis Bullosa

    OpenAIRE

    Jeon, In Kyung; On, Hye Rang; Kim, Soo-Chan

    2016-01-01

    Background Patients with recessive dystrophic epidermolysis bullosa (RDEB) exhibit blisters and erosions since birth, causing pain, pruritus and various complications. RDEB affects quality of life (QoL) in physical, emotional and social aspects. Furthermore, interminable dressing changes and supportive therapies impose a significant economic burden on the patient's family. Objective We assessed the QoL and economic burden in patients with RDEB. Methods Sixteen patients with RDEB were surveyed...

  9. Proactive or Reactive? Optimal Management of an Invasive Forest Pest in a Spatial Framework

    OpenAIRE

    Bond, Craig A.; Champ, Patricia A.; Meldrum, James; Schoettle, Anna

    2010-01-01

    This paper offers a preliminary investigation into the conditions under which it might be optimal to engage in proactive management of a non-timber forest resource in the presence of an invasive species whose spread is unaffected by management action. Proactive management is defined as treating an uninfected area in order to encourage healthy ecosystem function, given that the arrival of the invasive is inevitable. Inspired by the problem of white pine blister rust in the Rocky Mountain west,...

  10. Clinical, High Resolution Computed Tomography and Pulmonary Function in Sulphur Mustard Victims

    OpenAIRE

    Javad Moghimi; Armita Valizadeh; Habib Heybar; Mahdi Yadollahzadeh; Ahmad Ahmadzadeh; Esmaeil Idani

    2012-01-01

    We aimed to evaluate clinical, high resolution computed tomography (HRCT) and pulmonary function test (PFT) findings after 18-23 years of exposure in veterans of sulphur mustard (SM) exposure. We performed a cross-sectional study of 106 patients. Inclusion criteria were 1: documented exposure to SM as confirmed by toxicological analysis of their urine and vesicular fluid after exposure 2: single exposure to SM that cause skin blisters and subsequent transient or permanent sequel. Cigarette sm...

  11. Geospatial Variation of an Invasive Forest Disease and the Effects on Treeline Dynamics in the Rocky Mountains

    OpenAIRE

    Smith-McKenna, Emily Katherine

    2013-01-01

    Whitebark pine is an important keystone and foundation species in western North American mountain ranges, and facilitates tree island development in Rocky Mountain treelines. The manifestation of white pine blister rust in the cold and dry treelines of the Rockies, and the subsequent infection and mortality of whitebark pines raises questions as to how these extreme environments harbor the invasive disease, and what the consequences may be for treeline dynamics. This dissertation research c...

  12. Effects of strain and trapping on hydrogen-induced cracking in high strength low alloy steels

    OpenAIRE

    Bosch, Cédric; Delafosse, David; Longaygue, Xavier

    2010-01-01

    International audience In pearlitic steels, hydrogen trapping at interphase boundaries may induce hydrogen- cracking in the absence of external applied stress such as in blistering and Hydrogen Induced Cracking. However, in low alloy steels containing a much lower density of such trapping sites and/or when hydrogen activity is reduced, most instances of hydrogen-induced cracking involve strong interactions between local plasticity and hydrogen effects. We investigated the effects of these ...

  13. Effects of an introduced pathogen and fire exclusion on the demography of sugar pine

    Science.gov (United States)

    van Mantgem, Phillip J.; Stephenson, Nathan L.; Keifer, MaryBeth; Keeley, Jon E.

    2004-01-01

    An introduced pathogen, white pine blister rust (Cronartium ribicola), has caused declines in five-needled pines throughout North America. Simultaneously, fire exclusion has resulted in dense stands in many forest types, which may create additional stress for these generally shade-intolerant pines. Fire exclusion also allows fuels to accumulate, and it is unclear how affected populations will respond to the reintroduction of fire. Although white pine blister rust and fire exclusion are widely recognized threats, long-term demographic data that document the effects of these stressors are rare. We present population trends from 2168 individuals over 5–15 years for an affected species, sugar pine (Pinus lambertiana), at several burned and unburned sites in the Sierra Nevada of California. Size-based matrix models indicate that most unburned populations have negative growth rates (λ range: 0.82–1.04). The growth rate of most populations was, however, indistinguishable from replacement levels (λ = 1.0), implying that, if populations are indeed declining, the progression of any such decline is slow, and longer observations are needed to clearly determine population trends. We found significant differences among population growth rates, primarily due to variation in recruitment rates. Deaths associated with blister rust and stress (i.e., resource competition) were common, suggesting significant roles for both blister rust and fire exclusion in determining population trajectories. Data from 15 prescribed fires showed that the immediate effect of burning was the death of many small trees, with the frequency of mortality returning to pre-fire levels within five years. In spite of a poor prognosis for sugar pine, our results suggest that we have time to apply and refine management strategies to protect this species.

  14. Effects of helium irradiation on high heat load properties of tungsten

    International Nuclear Information System (INIS)

    He ion pre-implanted tungsten has been irradiated by electrons to examine synergistic effects of He implantation and high heat load. Depth profile measurements show that He distributes on the surface locally and most of He is distributed in the projected range, however, some of the helium atoms diffuse to deeper region. Blisters are observed on the sample irradiated to a fluence of 1x1022 He/m2 at 300 K. However, at 1073 K, such blisters are not observed and no noticeable modification occurs. There is no large difference in surface modification by electron beam irradiation between the samples irradiated or not with He when the sample is at the high temperature (2773 K) for 6 s. Two large He release peaks are observed from the sample irradiated to a fluence of 1x1022 He/m2 at 1073 K. It is thought that He release in the first peak is due to microcrack formation near the surface and that the second He release peak is due to the exfoliation or rupture of blisters

  15. Spontaneously-acoustic hypersound long-range stimulation of silicon nitride synthesis in silicon at argon ion irradiation

    CERN Document Server

    Demidov, E S; Markov, K A; Sdobnyakov, V V

    2001-01-01

    The work is dedicated to the nature of the average energy ions implantation process effect on the crystal defective system at the distances, exceeding by three-four orders the averagely projected ions run value. It is established that irradiation by the argon ions stimulated the Si sub 3 N sub 4 phase formation in the preliminarily nitrogen-saturated layers at the distances of approximately 600 mu m from the ions deceleration zone. It is supposed that there appear sufficiently effective pulse sources of the hypersonic shock waves in the area of the Ar sup + deceleration zone. These waves are the result of the jump-like origination and grid evolution of the loop-shaped dislocations and argon blisters as well as of the blisters explosion, The evaluations show that the peak pressure in wave due to the synchronized explosion of blisters in the nitrogen-saturated area on the reverse side of the silicon plate 600 mu m thick may exceed 10 sup 8 Pa and cause experimentally observed changes

  16. Helium Retention and Desorption Behaviour of Reduced Activation Ferritic/Martenstic Steel

    Science.gov (United States)

    Wang, Pinghuai; Nobuta, Yuji; Hino, Tomoaki; Yamauchi, Yuji; Chen, Jiming; Xu, Zengyu; Li, Xiongwei; Liu, Shi

    2009-04-01

    The reduced activation ferritic/martenstic steel CLF-1 prepared by the Southwestern Institute of Physics in China was irradiated by helium ions with an energy of 5 keV at room temperature using an electron cyclotron resonance (ECR) ion irradiation apparatus. After the irradiation, the helium retention and desorption were investigated using a technique of thermal desorption spectroscopy (TDS). The experiment was conducted with both the normal and welded samples. Blisters were observed after the helium ion irradiation, and the surface density of blisters in the welded samples was lower than that in the non-welded samples. Three desorption peaks were observed in both the non-welded and welded samples. These desorption peaks corresponded to those of blister ruptures and the helium release from the inner bubbles and the defects. The amount of helium retained in the welded samples was approximately the same as that in the non-welded samples, which was much less than other reduced activation materials, such as vanadium alloy and SiC/SiC composites.

  17. Influence of particle flux density and temperature on surface modifications of tungsten and deuterium retention

    Energy Technology Data Exchange (ETDEWEB)

    Buzi, Luxherta, E-mail: l.buzi@fz-juelich.de [Ghent University, Department of Applied Physics, Sint-Pietersnieuwstraat 41, B-9000 Ghent (Belgium); FOM Institute DIFFER-Dutch Institute for Fundamental Energy Research, Edisonbaan 14, 3439 MN, PO Box 1207, 3430 BE Nieuwegein (Netherlands); Institut für Energie und Klimaforschung – Plasmaphysik, Forschungszentrum Jülich GmbH, Leo-Brandt-Straße, 52425 Jülich (Germany); Université de Lorraine, Institut Jean Lamour, CNRS UMR 7198, Bvd. des Aiguillettes, F-54506 Vandoeuvre (France); Temmerman, Greg De [FOM Institute DIFFER-Dutch Institute for Fundamental Energy Research, Edisonbaan 14, 3439 MN, PO Box 1207, 3430 BE Nieuwegein (Netherlands); Unterberg, Bernhard; Reinhart, Michael; Litnovsky, Andrey; Philipps, Volker [Institut für Energie und Klimaforschung – Plasmaphysik, Forschungszentrum Jülich GmbH, Leo-Brandt-Straße, 52425 Jülich (Germany); Oost, Guido Van [Ghent University, Department of Applied Physics, Sint-Pietersnieuwstraat 41, B-9000 Ghent (Belgium); Möller, Sören [Institut für Energie und Klimaforschung – Plasmaphysik, Forschungszentrum Jülich GmbH, Leo-Brandt-Straße, 52425 Jülich (Germany)

    2014-12-15

    Systematic study of deuterium irradiation effects on tungsten was done under ITER – relevant high particle flux density, scanning a broad surface temperature range. Polycrystalline ITER – like grade tungsten samples were exposed in linear plasma devices to two different ranges of deuterium ion flux densities (high: 3.5–7 · 10{sup 23} D{sup +}/m{sup 2} s and low: 9 · 10{sup 21} D{sup +}/m{sup 2} s). Particle fluence and ion energy, respectively 10{sup 26} D{sup +}/m{sup 2} and ∼38 eV were kept constant in all cases. The experiments were performed at three different surface temperatures 530 K, 630 K and 870 K. Experimental results concerning the deuterium retention and surface modifications of low flux exposure confirmed previous investigations. At temperatures 530 K and 630 K, deuterium retention was higher at lower flux density due to the longer exposure time (steady state plasma operation) and a consequently deeper diffusion range. At 870 K, deuterium retention was found to be higher at high flux density according to the thermal desorption spectroscopy (TDS) measurements. While blisters were completely absent at low flux density, small blisters of about 40–50 nm were formed at high flux density exposure. At the given conditions, a relation between deuterium retention and blister formation has been found which has to be considered in addition to deuterium trapping in defects populated by diffusion.

  18. Helium Retention and Desorption Behaviour of Reduced Activation Ferritic/Martenstic Steel

    Institute of Scientific and Technical Information of China (English)

    WANG Pinghuai; NOBUTA Yuji; HINO Tomoaki; YAMAUCHI Yuji; CHEN Zilning; XU Zengyu; LI Xiongwei; LIU Shi

    2009-01-01

    The reduced activation ferritic/martenstic steel CLF-1 prepared by the Southwest-ern Institute of Physics in China was irradiated by helium ions with an energy of 5 keV at room temperature using an electron cyclotron resonance (ECR) ion irradiation apparatus. After the irradiation, the helium retention and desorption were investigated using a technique of thermal desorption spectroscopy (TDS). The experiment was conducted with both the normal and welded samples. Blisters were observed after the helium ion irradiation, and the surface density of blisters in the welded samples was lower than that in the non-welded samples. Three desorption peaks were observed in both the non-welded and welded samples. These desorption peaks corresponded to those of blister ruptures and the helium release from the inner bubbles and the defects. The amount of helium retained in the welded samples was approximately the same as that in the non-welded samples, which was much less than other reduced activation materials, such as vanadium alloy and SiC/SiC composites.

  19. Effect of Soil Drought Stress on Leaf Water Status, Membrane Permeability and Enzymatic Antioxidant System of Maize

    Institute of Scientific and Technical Information of China (English)

    BAI Li-Ping; SUI Fang-Gong; GE Ti-Da; SUN Zhao-Hui; LU Yin-Yan; ZHOU Guang-Sheng

    2006-01-01

    A simulation experiment on the responses of maize (Zea mays L.) from the third leaf stage to maturity for different soilwater levels (well-watered, moderately stressed, and severely stressed) was conducted by controlling irrigation and using a mobile rain shelter in a neutral loam, meadow soil to determine the effects on leaf water status, membrane permeability and enzymatic antioxidant system for different growth stages. The results indicated that drought stress relied on drought intensity and duration, with more severe drought stress creating more serious effects on maize. Compared with wellwatered conditions, during the silking and blister stages moderate stress did not significantly change the relative water content (RWC) and did change significantly the relative conductivity (RC) (P < 0.05) of the leaves; however, severestress did significantly decrease (P < 0.01) the leaf RWC and increase (P < 0.01) membrane permeability (leaf relative conductivity). Furthermore, under severe drought stress antioxidant enzyme activities declined significantly (P < 0.01) in later stages, namely for superoxide dismutase (SOD) the tasseling and blister stages, for peroxidase (POD) the milk stage, and for catalase (CAT) during the tasseling, blister, and milk stages. Meanwhile, membrane lipid peroxidation (measured as malondialdehyde content) significantly increased (P < 0.01) in all stages.

  20. Inherited epidermolysis bullosa

    Directory of Open Access Journals (Sweden)

    Fine Jo-David

    2010-05-01

    Full Text Available Abstract Inherited epidermolysis bullosa (EB encompasses a number of disorders characterized by recurrent blister formation as the result of structural fragility within the skin and selected other tissues. All types and subtypes of EB are rare; the overall incidence and prevalence of the disease within the United States is approximately 19 per one million live births and 8 per one million population, respectively. Clinical manifestations range widely, from localized blistering of the hands and feet to generalized blistering of the skin and oral cavity, and injury to many internal organs. Each EB subtype is known to arise from mutations within the genes encoding for several different proteins, each of which is intimately involved in the maintenance of keratinocyte structural stability or adhesion of the keratinocyte to the underlying dermis. EB is best diagnosed and subclassified by the collective findings obtained via detailed personal and family history, in concert with the results of immunofluorescence antigenic mapping, transmission electron microscopy, and in some cases, by DNA analysis. Optimal patient management requires a multidisciplinary approach, and revolves around the protection of susceptible tissues against trauma, use of sophisticated wound care dressings, aggressive nutritional support, and early medical or surgical interventions to correct whenever possible the extracutaneous complications. Prognosis varies considerably and is based on both EB subtype and the overall health of the patient.

  1. IgG BULLOUS PEMPHIGOID WITH ANTIBODIES TO IgD, DERMAL BLOOD VESSELS, ECCRINE GLANDS AND THE ENDOMYSIUM OF MONKEY ESOPHAGUS

    Directory of Open Access Journals (Sweden)

    Abreu Velez Ana Maria

    2011-04-01

    Full Text Available Context: Bullous pemphigoid is mediated by autoantibodies primarily targeting two structural proteins of basement membrane hemidesmosomes, BP180 (BPAG2; collagen XVII and BP230 (BPAG1. Case Report: A 70-year-old Caucasian male patient was evaluated for a seven day history of multiple itching, erythematous blisters on his extremities. Biopsies for hematoxylin and eosin examination, direct immunofluorescence and indirect immunofluorescence (including salt split skin analysis were performed. Results: Hematoxylin and eosin examination demonstrated a subepidermal blister. Within the blister lumen, numerous eosinophils and lymphocytes were noted. Direct and indirect immunofluorescence revealed linear deposits of IgG, Complement/C3 and fibrinogen at the basement membrane zone of the skin and surrounding selected dermal blood vessels and sweat glands. Positive intracytoplasmic staining for anti-human IgD was noted in most of the epidermis, as well as surrounding some dermal blood vessels. Indirect immunofluorescence utilizing monkey esophagus substrate demonstrated strong positivity within the endomysium for IgG antibodies. Conclusion: We report a unique case of bullous pemphigoid with reactivity to eccrine sweat glands, and selected dermal blood vessels. In addition, the observed reactivity of anti-human IgD, and of IgG to monkey esophagus endomysium warrant further investigation.

  2. RNA-seq analysis of host and viral gene expression highlights interaction between varicella zoster virus and keratinocyte differentiation.

    Directory of Open Access Journals (Sweden)

    Meleri Jones

    2014-01-01

    Full Text Available Varicella zoster virus (VZV is the etiological agent of chickenpox and shingles, diseases characterized by epidermal skin blistering. Using a calcium-induced keratinocyte differentiation model we investigated the interaction between epidermal differentiation and VZV infection. RNA-seq analysis showed that VZV infection has a profound effect on differentiating keratinocytes, altering the normal process of epidermal gene expression to generate a signature that resembles patterns of gene expression seen in both heritable and acquired skin-blistering disorders. Further investigation by real-time PCR, protein analysis and electron microscopy revealed that VZV specifically reduced expression of specific suprabasal cytokeratins and desmosomal proteins, leading to disruption of epidermal structure and function. These changes were accompanied by an upregulation of kallikreins and serine proteases. Taken together VZV infection promotes blistering and desquamation of the epidermis, both of which are necessary to the viral spread and pathogenesis. At the same time, analysis of the viral transcriptome provided evidence that VZV gene expression was significantly increased following calcium treatment of keratinocytes. Using reporter viruses and immunohistochemistry we confirmed that VZV gene and protein expression in skin is linked with cellular differentiation. These studies highlight the intimate host-pathogen interaction following VZV infection of skin and provide insight into the mechanisms by which VZV remodels the epidermal environment to promote its own replication and spread.

  3. Issues around the Prescription of Half Tablets in Northern Switzerland: The Irrational Case of Quetiapine

    Directory of Open Access Journals (Sweden)

    Samuel S. Allemann

    2015-01-01

    Full Text Available Background. Prescription of fragmented tablets is useful for individualisation of dose but includes several drawbacks. Although without score lines, the antipsychotic drug quetiapine was in 2011 the most often prescribed 1/2 tablet in discharge prescriptions at the University Hospital in Basel (USB, 671 beds. We aimed at analysing the prescription patterns of split tablets in general and of quetiapine in particular in Switzerland. Methods. All orders of community pharmacies for unit-of-use soft pouch blisters placed at Medifilm AG, the leader company in Switzerland for repackaging into pouch blisters, were analysed. Results. Out of 4,784,999 tablets that were repacked in 2012 in unit-of-use pouch blisters, 8.5% were fragmented, mostly in half (87.6%, and were predominantly psycholeptics (pipamperone 15.8%. Prescription of half quetiapine appears to be a Basel specificity (highest rates of fragments and half quetiapine. Conclusions. Prescription of fragmented tablet is frequent. It represents a safety issue for the patient, and a pharmaceutical care issue for the pharmacist. In ambulatory care, the patient’s cognitive and physical capacities must be clarified, suitability of the splitting of the tablet must be checked, appropriate aids must be offered, like a pill-splitting device in order to improve accuracy, and safe use of the drug must be ensured.

  4. AFIP-6 Breach Assessment Report

    Energy Technology Data Exchange (ETDEWEB)

    Dan Wachs; Adam Robinson; Pavel Medvedev

    2011-02-01

    Analysis of the AFIP-6 experiment is summarized in this report in order to determine the cause of gaseous fission product release observed during irradiation. During the irradiation, a series of small fission product releases were observed. In order to limit the potential for primary coolant contamination, the operating cycle was terminated and the AFIP-6 experiment was removed for examination. Both in-canal and post-irradiation examination revealed the presence of an unusually thick oxide layer and discrete surface blisters on the fuel plates. These blisters were the likely cause of fission product release. Subsequent detailed thermal hydraulic analysis of the experiment indicated that the combination of the high operating power and test vehicle configuration led to high nominal operating temperatures for the fuel plates. This elevated temperature led to accelerated surface corrosion and eventually spallation of the fuel plate cladding. The thermal insulating nature of this corrosion layer led to significantly elevated fuel meat temperatures that induced blistering. Analysis was performed to validate a corrosion rate model and criteria for onset of spallation type surface corrosion were determined. The corrosion rate model will be used to estimate the oxide thickness anticipated for experiments in the future. The margin to the spallation threshold will then be used to project the experiment performance.

  5. Desmocollin 3-mediated Binding Is Crucial for Keratinocyte Cohesion and Is Impaired in Pemphigus*

    Science.gov (United States)

    Spindler, Volker; Heupel, Wolfgang-Moritz; Efthymiadis, Athina; Schmidt, Enno; Eming, Rüdiger; Rankl, Christian; Hinterdorfer, Peter; Müller, Thomas; Drenckhahn, Detlev; Waschke, Jens

    2009-01-01

    Desmocollin (Dsc) 1–3 and desmoglein (Dsg) 1–4, transmembrane proteins of the cadherin family, form the adhesive core of desmosomes. Here we provide evidence that Dsc3 homo- and heterophilic trans-interaction is crucial for epidermal integrity. Single molecule atomic force microscopy (AFM) revealed homophilic trans-interaction of Dsc3. Dsc3 displayed heterophilic interaction with Dsg1 but not with Dsg3. A monoclonal antibody targeted against the extracellular domain reduced homophilic and heterophilic binding as measured by AFM, caused intraepidermal blistering in a model of human skin, and a loss of intercellular adhesion in cultured keratinocytes. Because autoantibodies against Dsg1 are associated with skin blistering in pemphigus, we characterized the role of Dsc3 binding for pemphigus pathogenesis. In contrast to AFM experiments, laser tweezer trapping revealed that pemphigus autoantibodies reduced binding of Dsc3-coated beads to the keratinocyte cell surface. These data indicate that loss of heterophilic Dsc3/Dsg1 binding may contribute to pemphigus skin blistering. PMID:19717567

  6. The observation of helium gas bubble lattices in copper, nickel and stainless steel

    International Nuclear Information System (INIS)

    Transmission electron microscopy is used to investigate the spatial arrangement of the small gas bubbles produced in several fcc metals by 30 keV helium ion irradiation to high dose at 300K. In what is a new result for this important class of metals it is found that the helium gas bubbles lie on a superlattice having an fcc structure with principal axes aligned with those of the metal matrix. The bubble lattice constant, asub(l), is measured for a helium fluence just below the critical dose for radiation blistering of the metal surface. Implantation rates are typically approximately 1014 He ions cm-2 sec-1. The values of asub(l) obtained for copper, nickel and stainless steel are given. Above the critical dose the bubble lattice is seen to survive in some blister caps as well as in the region between blisters. Bubble alignment is also observed in the case of hydrogen bubbles produced in copper by low energy proton irradiation to high fluence at 300K. (author)

  7. Observations of a F.C.C. helium gas-bubble superlattice in copper, nickel and stainless steel

    International Nuclear Information System (INIS)

    Transmission electron microscopy is used to investigate the spatial arrangement of the small gas bubbles produced in several fcc metals by 30 keV helium ion irradiation to high dose at 300 K. In what is a new result for this important class of metals, it is found that the helium gas bubbles lie on a superlattice having an fcc structure with principal axes aligned with those of the metal matrix. The bubble lattice constant asub(l), is measured for a helium fluence just below the critical dose for radiation blistering of the metal surface (approximately 4 x 1017 He/cm2). Implantation rates are typically approximately 1014 He ions cm-2 sec-1. The values of asub(l) obtained for copper, nickel and stainless steel are (7.6 +- 0.3) nm, (6.6 +- 0.5) nm and (6.4 +- 0.5) nm respectively. Above the critical dose, the bubble lattice is seen to survive in some blister caps as well as in the region between blisters. Bubble alignment is also observed in the case of hydrogen bubbles produced in copper by low energy proton irradiation to high fluence at 300K. The presentation of this data was accompanied by a cine film illustrating the behaviour of the gas bubble lattice in copper during post-irradiation annealing in the electron microscope. A summary of the film is given in the appendix. (author)

  8. Effects of exotic species on Yellowstone's grizzly bears

    Science.gov (United States)

    Reinhart, D.P.; Haroldson, Mark A.; Mattson, D.J.; Gunther, Kerry A.

    2001-01-01

    Humans have affected grizzly bears (Ursus arctos horribilis) by direct mortality, competition for space and resources, and introduction of exotic species. Exotic organisms that have affected grizzly bears in the Greater Yellowstone Area include common dandelion (Taraxacum officinale), nonnative clovers (Trifolium spp.), domesticated livestock, bovine brucellosis (Brucella abortus), lake trout (Salvelinus namaycush), and white pine blister rust (Cronartium ribicola). Some bears consume substantial amounts of dandelion and clover. However, these exotic foods provide little digested energy compared to higher-quality bear foods. Domestic livestock are of greater energetic value, but use of this food by bears often leads to conflicts with humans and subsequent increases in bear mortality. Lake trout, blister rust, and brucellosis diminish grizzly bears foods. Lake trout prey on native cutthroat trout (Oncorhynchus clarkii) in Yellowstone Lake; white pine blister rust has the potential to destroy native whitebark pine (Pinus albicaulis) stands; and management response to bovine brucellosis, a disease found in the Yellowstone bison (Bison bison) and elk (Cervus elaphus), could reduce populations of these 2 species. Exotic species will likely cause more harm than good for Yellowstone grizzly bears. Managers have few options to mitigate or contain the impacts of exotics on Yellowstones grizzly bears. Moreover, their potential negative impacts have only begun to unfold. Exotic species may lead to the loss of substantial highquality grizzly bear foods, including much of the bison, trout, and pine seeds that Yellowstone grizzly bears currently depend upon.

  9. Chroococcales em Esteiras Microbianas em Bolha do Brejo do Pau Fincado, Rio de Janeiro, Brasil.

    Directory of Open Access Journals (Sweden)

    Loreine Hermida da Silva e Silva

    2007-07-01

    Full Text Available Chroococcales in blister microbial mats at brejo do Pau Fincado, Rio de Janeiro, Brazil.Brejo do Pau Fincado (220 55’ 56,75” S e 420 16’ 54,7” W is a natural natural coastal aquatical body. It is located at the Lagoa de Araruama complex, formed at least 7000 years ago. The local climate is characterized by low precipitation ratesand high evaporation rates; due to this, the lagoons tend to become ypersalyne. Microbial mats are organic-sedimentary structures composed mainly of cyanobacteria. Whilst the photosynthesis process cyanobacteria induct an environmental change, which leads to calcium carbonate precipitation. The aim of this study was to typify and identify the cyanobacteria at the blister microbial mat. Samples were processed and analyzed using an optical microscope. The blister mat is composed of twelve species of cyanobacteria disposed in a fragile and amorphous mass. In this structure, both quantitative andqualitative domains belong to Chroococcales Westtein 1924. Measurement has shown that the place is very selective, andcyanobacteria presented great resistance and adaptability, because they are forms which had got success surviving to big changes occurred on Earth since the beginning of life and the evolutionary history .

  10. The impact of graded levels of day length on turkey health and behavior to 18 weeks of age.

    Science.gov (United States)

    Vermette, C; Schwean-Lardner, K; Gomis, S; Grahn, B H; Crowe, T G; Classen, H L

    2016-06-01

    The impact of graded levels of day length on turkey health and behavior was determined in hens and toms raised to 18 wk of age. Birds were allocated to one of 4 lighting treatments (trt) providing 14 (14L), 17 (17L), 20 (20L), and 23 (23L) h of day length. Two time-replicated trials were completed with each, providing 2 rooms per lighting trt and each room having 3 hen (n = 720) and 3 tom (n = 480) pens. Data collection included gait score (GS), the incidence of footpad dermatitis (FPD), breast buttons and blisters, ocular size and pressure (males only), and behavioral observations (males only). Data were analyzed using SAS 9.3 based on a completely randomized design nested within 4 lighting trt. Regression analysis established relationships between response criteria and day length. Differences were considered significant at P ≤ 0.05 and trends noted at P ≤ 0.10. Gait score, FPD, and the incidence of breast buttons and blisters were assessed on 5 birds per pen at 11 and 17 wk of age. Average GS increased linearly with day length at 11 and 17 wk for both hens and toms, but the effect was larger in toms. Day length did not affect FPD, but more lesions and severe scores were found for hens than toms. The presence of breast buttons and blisters increased linearly with day length (11 wk) with the effect on blisters predominately seen in toms. Eye weight and size increased with increasing day length at 12 and 18 wk. Ocular pressure was not affected by day length. Infrared cameras recorded tom behavior over a 24 h period, and behaviors were classified over 10-min intervals using a scan sampling technique at 11 and 17 wk. Alterations in behavioral repertoire were observed with a linear increase in inactive resting and a linear decrease in standing, walking, preening, and environmental and feather pecking behaviors with increased day length. To conclude, day length affected bird mobility and incidence of breast blisters in an age- and gender-specific manner, and time

  11. Clinical variability in dystrophic epidermolysis bullosa and findings with scanning electron microscopy Variabilidade clínica em epidermólise bolhosa distrófica e achados de microscopia eletrônica de varredura

    Directory of Open Access Journals (Sweden)

    Hiram Larangeira de Almeida Jr

    2012-02-01

    Full Text Available In dystrophic epidermolysis bullosa, the genetic defect of anchoring fibrils leads to cleavage beneath the basement membrane and its consequent loss. A 46 year-old female patient presented blisters with a pretibial distribution associated with nail dystrophy. Her two children had hyponychia and anonychia, which affected all toe nails and the thumb, forefinger and middle finger. DNA sequencing identified in exon 75 of COL7A1 gene a pathologic mutation: c.6235G>A (p.Gly2079Arg. Immunomapping of a blister demonstrated collagen IV (basal membrane in the blister roof and collagen VII in its floor, confirming dystrophic epidermolysis bullosa. Scanning electron microscopy of an inverted blister showed net-forming collagen attached to the blister roof . The variability found in this family has already been reported and confirms, on a clinical basis, the nail subtype as a dystrophic variant.Na epidermólise bolhosa distrófica, o defeito genético das fibrilas de ancoragem leva à clivagem abaixo da membrana basal com sua consequente perda. Uma paciente de 46 anos apresentava bolhas pré-tibiais associadas à distrofia ungueal. Seus dois filhos apresentavam hipo e anoníquia, afetando todas as unhas dos pododáctilos e dos primeiros, segundos e terceiros quirodáctilos. O sequenciamento de DNA identificou no exon 75 do gene COL7A1 uma mutação patológica: c.6235G>A (p.Gly2079Arg. O imunomapeamento identificou o colágeno IV no teto e colágeno VII no assoalho de uma bolha , confirmando o diagnóstico de epidermólise bolhosa distrófica. A microscopia eletrônica de varredura de um teto invertido de bolha demonstrou rede de colágeno aderida ao mesmo. A variabilidade clínica encontrada nessa família já foi escrita e confirma, que o subtipo ungueal das epidermólises bolhosas é uma forma distrófica.

  12. Air sensitivity of MoS2, MoSe2, MoTe2, HfS2, and HfSe2

    Science.gov (United States)

    Mirabelli, Gioele; McGeough, Conor; Schmidt, Michael; McCarthy, Eoin K.; Monaghan, Scott; Povey, Ian M.; McCarthy, Melissa; Gity, Farzan; Nagle, Roger; Hughes, Greg; Cafolla, Attilio; Hurley, Paul K.; Duffy, Ray

    2016-09-01

    A surface sensitivity study was performed on different transition-metal dichalcogenides (TMDs) under ambient conditions in order to understand which material is the most suitable for future device applications. Initially, Atomic Force Microscopy and Scanning Electron Microscopy studies were carried out over a period of 27 days on mechanically exfoliated flakes of 5 different TMDs, namely, MoS2, MoSe2, MoTe2, HfS2, and HfSe2. The most reactive were MoTe2 and HfSe2. HfSe2, in particular, showed surface protrusions after ambient exposure, reaching a height and width of approximately 60 nm after a single day. This study was later supplemented by Transmission Electron Microscopy (TEM) cross-sectional analysis, which showed hemispherical-shaped surface blisters that are amorphous in nature, approximately 180-240 nm tall and 420-540 nm wide, after 5 months of air exposure, as well as surface deformation in regions between these structures, related to surface oxidation. An X-ray photoelectron spectroscopy study of atmosphere exposed HfSe2 was conducted over various time scales, which indicated that the Hf undergoes a preferential reaction with oxygen as compared to the Se. Energy-Dispersive X-Ray Spectroscopy showed that the blisters are Se-rich; thus, it is theorised that HfO2 forms when the HfSe2 reacts in ambient, which in turn causes the Se atoms to be aggregated at the surface in the form of blisters. Overall, it is evident that air contact drastically affects the structural properties of TMD materials. This issue poses one of the biggest challenges for future TMD-based devices and technologies.

  13. Study of neutronic effects of water hole in the control fuel element in the Tehran research reactor

    International Nuclear Information System (INIS)

    Due to the existence of some fission products in the core outlet of the Tehran Research Reactor, fuel leakage tests were done on all fuel elements. These experiments showed that the fission products come out mostly from some of the fuel control elements. So the failures of fuel control elements were taken out of the core and reactor started with the new core configuration. Failure of fuel plate in pool type research reactors resulting in a release of fission products to the pool water fall into the following categories: 1. Blistering or swelling due to excessive fission gas or fission products build-up. 2. Failure due to corrosion (chemical-metallurgical, ...). 3. Manufacturing defect such as non-homogeneous fuel distribution. 4. Coolant channel blockage by foreign materials. From neutronic point of view, existance of water hole in the CFE causes increasing thermal flux on the fuel plate adjusted to the water hole. Therefore, there is a probability of blistering or swelling in some parts of the plates. For more studies of this effect, we did neutronic calculations using computer codes. Calculation also showed that the fission rate in the above mentioned plate in the CFE is more than other plates in the core. Therefore blistering or swelling due to excessive fission gas or fission products build-up in some part of the plate is probable. As it was mentioned before, the probability of chemical and metallurgical corrosion on the fuel clad should not be ignored. However, since the release of fission products from some of the CFE were more than other fuel assemblies, the probability of occuring cladding defects by the neutronic effects of the water hole is stronger than other effects. (Author)

  14. Assessment of reactivity transient experiments with high burnup fuel

    Energy Technology Data Exchange (ETDEWEB)

    Ozer, O.; Yang, R.L.; Rashid, Y.R.; Montgomery, R.O.

    1996-03-01

    A few recent experiments aimed at determining the response of high-burnup LWR fuel during a reactivity initiated accident (RIA) have raised concerns that existing failure criteria may be inappropriate for such fuel. In particular, three experiments (SPERT CDC-859, NSRR HBO-1 and CABRI REP Na-1) appear to have resulted in fuel failures at only a fraction of the anticipated enthalpy levels. In evaluating the results of such RIA simulation experiments, however, it is necessary that the following two key considerations be taken into account: (1) Are the experiments representative of conditions that LWR fuel would experience during an in-reactor RIA event? (2) Is the fuel that is being utilized in the tests representative of the present (or anticipated) population of LWR fuel? Conducting experiments under conditions that can not occur in-reactor can trigger response modes that could not take place during in-reactor operation. Similarly, using unrepresentative fuel samples for the tests will produce failure information that is of limited relevance to commercial LWR fuel. This is particularly important for high-burnup fuel since the manner under which the test samples are base-irradiated prior to the test will impact the mechanical properties of the cladding and will therefore affect the RIA response. A good example of this effect can be seen in the results of the SPERT CDC-859 test and in the NSRR JM-4 and JM-5 tests. The conditions under which the fuel used for these tests was fabricated and/or base-irradiated prior to the RIA pulse resulted in the formation of multiple cladding defects in the form of hydride blisters. When this fuel was subjected to the RIA power pulse, it failed by developing multiple cracks that were closely correlated with the locations of the pre-existing hydride blisters. In the case of the JM tests, many of the cracks formed within the blisters themselves and did not propagate beyond the heavily hydrided regions.

  15. Local cytokine changes in complex regional pain syndrome type I (CRPS I) resolve after 6 months.

    Science.gov (United States)

    Lenz, Melanie; Uçeyler, Nurcan; Frettlöh, Jule; Höffken, Oliver; Krumova, Elena K; Lissek, Silke; Reinersmann, Annika; Sommer, Claudia; Stude, Philipp; Waaga-Gasser, Ana M; Tegenthoff, Martin; Maier, Christoph

    2013-10-01

    There is evidence that inflammatory processes are involved in at least the early phase of complex regional pain syndrome (CRPS). We compared a panel of pro- and antiinflammatory cytokines in skin blister fluids and serum from patients with CRPS and patients with upper-limb pain of other origin (non-CRPS) in the early stage (< 1 year) and after 6 months of pain treatment. Blister fluid was collected from the affected and contralateral nonaffected side. We used a multiplex-10 bead array cytokine assay and Luminex technology to measure protein concentrations of the cytokines interleukin-1 receptor antagonist (IL-1RA), IL-2, IL-6, IL-8, IL-10, IL-12p40, and tumor necrosis factor-alpha (TNF-α) and the chemokines eotaxin, monocyte chemotactic protein-1 (MCP-1), and macrophage inflammatory protein-1β (MIP-1β). We found bilaterally increased proinflammatory TNF-α and MIP-1β and decreased antiinflammatory IL-1RA protein levels in CRPS patients compared to non-CRPS patients. Neither group showed side differences. After 6 months under analgesic treatment, protein levels of all measured cytokines in CRPS patients, except for IL-6, significantly changed bilaterally to the level of non-CRPS patients. These changes were not related to treatment outcome. In serum, only IL-8, TNF-α, eotaxin, MCP-1, and MIP-1β were detectable without intergroup differences. Blister fluid of CRPS patients showed a bilateral proinflammatory cytokine profile. This profile seems to be relevant only at the early stage of CRPS. Almost all measured cytokine levels were comparable to those of non-CRPS patients after 6 months of analgesic treatment and were not related to treatment outcome.

  16. HLA-DPDQDR is expressed in all lesional skin from patients with autoimmune skin diseases

    Directory of Open Access Journals (Sweden)

    Ana Maria Abreu Velez

    2014-04-01

    Full Text Available Introduction: Human genes responsible for human antigen presentation and transplant rejection functions are located on the short arm of Chromosome 6 and are called the Major Histocompatibility Complex (MHC. Moreover, the primary physiologic function of MHC molecules is to present peptides to T lymphocytes. MHC molecules are integral components of the ligands that most T cells recognize, since the T cell receptor (TCR has specificity for complexes of foreign antigenic peptides, as well as self-MHC molecules. Aim: Our investigation attempts to investigate the presence of HLA-DPDQDR within lesional skin biopsies from patients affected by autoimmune skin blistering diseases (ABDs. Materials and Methods: We utilized immunohistochemistry (IHC to evaluate the presence of HLA-DPDQDR in lesional skin biopsies of patients affected by ABDs. We tested 30 patients with endemic pemphigus foliaceus (EPF, 15 controls from the EPF endemic area, and 15 biopsies from healthy controls from the USA. We also tested archival biopsies from patients with selected ABDs, including 30 patients with bullous pemphigoid (BP, 20 with pemphigus vulgaris (PV, 8 with pemphigus foliaceus (PF, 14 with dermatitis herpetiformis (DH and 2 with epidermolysis bullosa acquisita (EBA. Results: Most ABD biopsies stained positive for HLA-DPDQDR in the lesional blisters and/or inflamed neurovascular plexus in the superficial dermis, and also at mesenchymal-endothelial like-cell junctions in the dermis. In BP, EBA and EPF, the HLA-DPDQDR staining was also seen in the dermal eccrine sweat gland coils and and ducts. Conclusion: Here, we document that HLA-DPDQDR is expressed in several anatomic areas of lesional skin in patients with ABDs. Notably, HLA-DPDQDR positivity was also consistently present in areas of the classic immune response in pemphigus epidermal keratinocytic intercellular junctions, and at basement membrane sites in bullous pemphigoid and other subepidermal blistering diseases.

  17. Skin microbiota-associated inflammation precedes autoantibody induced tissue damage in experimental epidermolysis bullosa acquisita.

    Science.gov (United States)

    Ellebrecht, Christoph T; Srinivas, Girish; Bieber, Katja; Banczyk, David; Kalies, Kathrin; Künzel, Sven; Hammers, Christoph M; Baines, John F; Zillikens, Detlef; Ludwig, Ralf J; Westermann, Jürgen

    2016-04-01

    Epidermolysis bullosa acquisita (EBA) is a chronic autoimmune blistering skin disease characterized by autoantibodies against type VII collagen (COL7). Immunization of SJL/J mice with recombinant murine COL7 results in break of tolerance and skin blisters. Strikingly, despite circulating autoantibodies, the same genetic background and identical environmental conditions, 20% of mice remain healthy. To elucidate the regulation of the transition from the presence of autoantibodies to overt autoimmune disease, we characterized the innate and adaptive immune response of mice that remain healthy after immunization and compared it to mice that developed skin disease. Both clinically healthy and diseased SJL/J mice showed circulating autoantibodies and deposition of complement-fixing IgG2c autoantibodies and C3 at the dermal-epidermal junction. However, only in diseased animals significant neutrophil infiltration and increase in FcgRIV expression were observed in the skin. In contrast, the expression of T cell signature cytokines in the T cell zone of the draining lymph node was comparable between clinically healthy and diseased animals after immunization. Surprisingly, health was associated with a decreased expression of CD11c, TNFA and KC (CXCL1) in the skin prior to immunization and could be predicted with a negative predictive value of >80%. Furthermore, mice that did not develop clinical disease showed a significantly higher richness and distinctly clustered diversity of their skin microbiota before immunization. Our data indicate that the decision whether blisters develop in the presence of autoantibodies is governed in the skin rather than in the lymph node, and that a greater richness of cutaneous bacterial species appears to be protective. PMID:26341384

  18. Light and transmission electron microscopy of generalized dystrophic epidermolysis bullosa (Pasini's albopapuloid subtype Microscopia óptica e eletrônica de transmissão da epidermólise bolhosa distrófica generalizada (subtipo albo-papulóide de Pasini

    Directory of Open Access Journals (Sweden)

    Hiram Larangeira de Almeida Jr

    2012-04-01

    Full Text Available Pasini's albopapuloid epidermolysis bullosa is a very rare subtype of generalized dystrophic dominant epidermolyis bullosa. A 30 year-old white female patient presented since her childhood disseminated small blisters and papules. Light microscopy of a blister showed dermal-epidermal cleavage; moreover, focal areas of dermal-epidermal splitting were also observed. Transmission electron microscopy also identified focal areas of cleavage, which were seen below the lamina densa. It is important to recognize this condition as a variant of epidermolysis bullosa, since the most important cutaneous findings are generalized papules and not blisters and erosions as in other forms of epidermolysis bullosa.A epidermólise bolhosa albo-papulóide de Pasini é uma variante rara da forma generalizada de epidermólise bolhosa distrófica dominante. Uma paciente de 30 anos apresenta desde a infância pápulas e bolhas disseminadas. A microscopia óptica de uma bolha demonstrou clivagem dermo-epidérmica; além disso áreas focais de desprendimento dermo-epidérmico foram encontradas. A microscopia eletrônica de transmissão identificou a clivagem abaixo da lâmina densa. É importante que se reconheça essa variante de epidermólise bolhosa, já que o aspecto clínico predominante são pápulas disseminadas e não bolhas como nas outras formas de epidermólise bolhosa.

  19. Epidermólise bolhosa adquirida inflamatória: relato de caso Inflammatory epidermolysis bullosa acquisita: case report

    Directory of Open Access Journals (Sweden)

    Marcelo D'Ambrosio Fernandes

    2009-04-01

    Full Text Available Apresenta-se caso de epidermólise bolhosa adquirida inflamatória. Paciente do sexo masculino, 53 anos, há seis meses com erupção vesicobolhosa pruriginosa sobre base eritematosa no tronco, axilas e membros. O exame anatomopatológico mostrou bolha subepidérmica com neutrófilos. A imunofluorescência direta revelou depósitos lineares de IgG, IgA, IgM e C3 na zona da membrana basal, sendo a imunofluorescência indireta e o Salt Split Skin indireto negativos. Anticorpos antinucleares não reagentes. Houve melhora do quadro com prednisona e cicatrização de algumas lesões com formação de milia. Trata-se de apresentação rara de epidermólise bolhosa adquirida, com lesões iniciais predominantemente inflamatórias.We report a case of an inflammatory variant of epidermolysis bullosa acquisita in a 53-year-old male, with itching blistering eruption on the trunk, armpits and limbs for six months. The skin biopsy specimen showed subepidermal blister with neutrophils. Direct immunofluorescence revealed linear depositions of IgG, IgA, IgM and C3 at the basement membrane; indirect immunofluorescence and salt Split Skin were negative. Antinuclear antibodies were also negative. Improvement of the blisters followed treatment with systemic corticotherapy and some lesions healed with milia. This is a rare presentation of epidermolysis bullosa acquisita, with inflammatory lesions at first.

  20. Investigation on the passivated Si/Al2O3 interface fabricated by non-vacuum spatial atomic layer deposition system.

    Science.gov (United States)

    Lien, Shui-Yang; Yang, Chih-Hsiang; Wu, Kuei-Ching; Kung, Chung-Yuan

    2015-01-01

    Currently, aluminum oxide stacked with silicon nitride (Al2O3/SiNx:H) is a promising rear passivation material for high-efficiency P-type passivated emitter and rear cell (PERC). It has been indicated that atomic layer deposition system (ALD) is much more suitable to prepare high-quality Al2O3 films than plasma-enhanced chemical vapor deposition system and other process techniques. In this study, an ultrafast, non-vacuum spatial ALD with the deposition rate of around 10 nm/min, developed by our group, is hired to deposit Al2O3 films. Upon post-annealing for the Al2O3 films, the unwanted delamination, regarded as blisters, was found by an optical microscope. This may lead to a worse contact within the Si/Al2O3 interface, deteriorating the passivation quality. Thin stoichiometric silicon dioxide films prepared on the Si surface prior to Al2O3 fabrication effectively reduce a considerable amount of blisters. The residual blisters can be further out-gassed when the Al2O3 films are thinned to 8 nm and annealed above 650°C. Eventually, the entire PERC with the improved triple-layer SiO2/Al2O3/SiNx:H stacked passivation film has an obvious gain in open-circuit voltage (V oc) and short-circuit current (J sc) because of the increased minority carrier lifetime and internal rear-side reflectance, respectively. The electrical performance of the optimized PERC with the V oc of 0.647 V, J sc of 38.2 mA/cm(2), fill factor of 0.776, and the efficiency of 19.18% can be achieved.

  1. PCR联合透射电镜诊断口唇初发单纯疱疹一例%Diagnosis of primary herpes simplex in the upper lip with PCR and transmission electron microscopy: a case report

    Institute of Scientific and Technical Information of China (English)

    李聪慧; 冉玉平; 雷松; 丁柳; 王鹏

    2012-01-01

    A 66-year-old man was admitted for a 7-day history of painful blisters and erosions in the upper lip.Real-time PCR with herpes simplex virus (HSV) type-specific primers showed that the blister fluid and crusts were positive for HSV-1,but negative for HSV-2.Observation of the blister wall with transmission electron microscopy (TEM) revealed 3 types of nucleocapsid in the karyoplast of epithelia cells,including the electron-translucent core,granular core and electron-dense core.Numerous matured viral particles with envelope were found in the cytoplasm,which were identified as HSV.The diagnosis was made as herpes simplex in the upper lip based on the above findings.PCR based molecular typing combined with observation of HSV particles via TEM may be an effective approach to the definite diagnosis of primary herpes simplex.%患者男,66岁,因上唇水疱、糜烂伴疼痛7d就诊.取水疱及痂皮,利用单纯疱疹病毒(HSV)特异性引物做实时荧光PCR检测,发现HSV-1阳性、HSV-2阴性.取疱壁做透射电镜观察,见表皮细胞核内有空心型、颗粒型或致密型3种类型的核衣壳,细胞核外大量成熟病毒颗粒,鉴定为疱疹病毒.诊断:口唇单纯疱疹.PCR分子分型结合透射电镜是确诊初发单纯疱疹的有效方法.

  2. Acute compartment syndrome of the forearm and hand in a patient of spine surgery -A case report-

    OpenAIRE

    Lee, Jung-ah; Jeon, Yeon Soo; Jung, Hong Soo; Kim, Hyung-Gun; Kim, Yong Shin

    2010-01-01

    A 38-year-old woman underwent a 4-hour operation in the prone position for a laminectomy at C4-7 and posterior cervical decompressive fusion at C7-T1 under general anesthesia. After undraping at the end of surgery, considerable swelling with many blisters of the left forearm and hand was observed. The chest roll at the left side had moved cephalad into the axilla and compressed the axillary structures. An emergency fasciotomy to decompress the compartments of the forearm and dorsal surface of...

  3. Preparation of naphtalene monocrystals for scintillometers

    International Nuclear Information System (INIS)

    A method to prepare naphthalene crystals for scintillometers is described. The different methods of preparation and the reasons to choose the Bridgman method are analysed. The thermal curves of the growing furnaces, blister shape, use of curved capillaries and the most convenient growing velocities are studied in detail. Methods of preparation of high purity naphthalene by means of chromatography and zone melting are described. Finally the operations of cutting and polishing of crystal and their most significant physics properties are studied. (Author) 28 refs

  4. 双侧带状疱疹案%Case of bilateral herpes zoster

    Institute of Scientific and Technical Information of China (English)

    艾明媚; 张品; 李岩

    2010-01-01

    @@ The patient, female, 56 years old, born in Jilin provine, northeast of China, paid her first visit to Tianjing Public Security Hospital on August 24,2009. Main complaints: blisters and pain on left chest and back, right flank and hypochondriac region for over 10 days. Medical history: burning pain on left shoulder and back without any obvious precipitating cause 10 days ago. Agminate and red papulo-vesicles from the size of millet to green bean appeared along the 4th intercostals space on the back the next day.

  5. Localized bullous pemphigoid on sites of radiotherapy and lymphedema in the same patient

    Directory of Open Access Journals (Sweden)

    Manikoth P Binitha

    2014-01-01

    Full Text Available Bullous pemphigoid (BP is a blistering disorder due to autoantibodies to the epidermal basement membrane zone. The triggering factor could be localized damage to the skin by physical or chemical agents. We report a case of a 68-year-old woman with a three year history of oral lesions of BP following radiotherapy for carcinoma of the hypopharynx, and a three month history of BP over lymphedematous sites on the right hand and right lower limb. Localized BP induced by radiotherapy or lymphedema is rare; both factors working simultaneously in the same patient is even rarer.

  6. Thermohydraulic characteristics analysis of natural convective cooling mode on the steady state condition of upgraded JRR-3 core, using COOLOD-N code

    International Nuclear Information System (INIS)

    This report describes the results of the steady state thermohydraulic analysis of upgraded JRR-3 core under natural convective cooling mode, using COOLOD-N code. In the code, function to calculate flow-rate under natural convective cooling mode, and a heat transfer package have been newly added to the COOLOD code which has been developed in JAERI. And this report describes outline of the COOLOD-N code. The results of analysis show that the thermohydraulics of upgraded JRR-3 core, under natural convective cooling mode have enough margine to ONB temperature, DNB heat flux and occurance of blisters in fuel meats, which are design criterion of upgraded JRR-3. (author)

  7. Results after 19 years of testing in a Pinus strobus x P. wallichiana F1 hybrid population

    OpenAIRE

    I. Blada; Popescu, F.

    2013-01-01

    Full-sib families were generated from a factorial design between seven female trees of P. strobus and four male trees of P. wallichiana to combine the rapid growth of former species with high resistance to blisterrust (Cronartium ribicola) of the latter one. At age two the hybrid families were artificially inoculated with blister-rust and at age six theywere field planted. Total height growth, diameter at 1.3 m, basal area, stem volume, stem straightness and tree survival were measuredat age ...

  8. Carbon enhanced hydriding of oxidized U-0.1wt%Cr surfaces

    Science.gov (United States)

    Shamir, N.; Schweke, D.; Rubin, A.; Livneh, T.; Zalkind, S.

    2010-03-01

    The effect of deposited amorphous carbon on the reactivity of uranium towards hydridization has been investigated by means of optical (HSM), electron (SEM), Raman scattering and atomic force (AFM) microscopies. Clear preference was found towards hydrogen attack on the carbon covered area as mostly manifested by the formation of hollow blister-like hydrides with average height of ~1μm, and crust of few hundreds of nm. NOTE: As a result of an inadvertent AN2009 editorial error that led to publication of non-finalized version the PDF was replaced with the correct version on 16 September 2010. The original PDF can be found in the supplementary data.

  9. Lichen planus with bullous manifestation on the lip.

    Science.gov (United States)

    van Tuyll van Serooskerken, Anne-Moon; van Marion, Arienne M W; de Zwart-Storm, Eugene; Frank, Jorge; Poblete-Gutiérrez, Pamela

    2007-11-01

    A 75-year-old Caucasian female with a previous history of cutaneous lichen planus (LP) developed vesiculae and blisters on the lip. This special variant of oral LP is very rare and has to be distinguished from LP pemphigoides, bullous pemphigoid, pemphigus vulgaris, erythema multiforme, and herpes simplex virus infection. To date, there are only few reports on bullous oral LP on the lower lip. Although treatment is difficult, we successfully applied a topical combination of tretinoin 0.025% and triamcinolone 0.1%. PMID:17973884

  10. Bullous Systemic Lupus Erythematosus: Case report

    OpenAIRE

    Miziara, Ivan Dieb; Mahmoud, Ali; Chagury, Azis Arruda; Alves, Ricardo Dourado

    2013-01-01

    Summary Introduction: Bullous systemic lupus erythematosus (BSLE) is an autoantibody-mediated disease with subepidermal blisters. It is a rare form of presentation of SLE that occurs in less than 5% of cases of lupus. Case Report: A 27-year-old, female, FRS patient reported the appearance of painful bullous lesions in the left nasal wing and left buccal mucosa that displayed sudden and rapid growth. She sought advice from emergency dermatology staff 15 days after onset and was hospitalized wi...

  11. Caso para diagnóstico Case for Diagnosis

    OpenAIRE

    Kleber de Sousa Silveira; Renata Indelicato Zac; Patrícia Jannuzzi Vieira e Oliveira; Daniela Rezende Neves; Valéria Gomes Barbosa; Maria Ester Massara Café

    2009-01-01

    Relata-se o caso de paciente do sexo feminino, de 9 anos de idade, com história de lesões bolhosas exclusivamente no dimídio direito, que acomete vários familiares de forma simétrica e cuja histologia evidencia acantólise tipo muro dilapidado, correspondendo, assim, a uma apresentação rara de mosaicismo na doença de Hailey-Hailey.The case of a 9-year-old girl with a history of blistering lesions restricted to the right side of her body is described. Several family members are also affected in...

  12. Human keratinocyte ATP2C1 localizes to the Golgi and controls Golgi Ca2+ stores

    OpenAIRE

    Behne, M J; Tu, Chia-Ling L; Aronchik, I; Epstein, E; Bench, G.; Bikle, D D; Pozzan, T; Mauro, T.M.

    2003-01-01

    Hailey-Hailey disease (MIM16960) is a blistering skin disease caused by mutations in the Ca2+ ATPase ATP2C1. We found that the abnormal Ca2+ signaling seen in Hailey-Hailey disease keratinocytes correlates with decreased protein levels of ATP2C1. Human ATP2C1 protein approximated 115 kDa in size. The ATP2C1 is localized to the Golgi apparatus in human keratinocytes, similar to its localization in yeast and Caenorhabditis elegans. To test whether the ATP2C1 controls Golgi Ca2+ stores, we measu...

  13. Narrowing of the Hailey-Hailey disease gene region on chromosome 3q and identification of one kindred with a deletion in this region

    Energy Technology Data Exchange (ETDEWEB)

    Peluso, A.M.; Bonifas, J.M.; Ikeda, S.; Hu, Z. [Univ. of California, San Francisco, CA (United States)] [and others

    1995-11-01

    Hailey-Hailey disease is a cutaneous abnormality transmitted as an autosomal dominant trait in which impaired interkeratinocyte adhesion produces recurrent blisters in characteristic skin sites. We report here a confirmation of the initial mapping of the mutant gene to chromosome 3q in an additional seven kindreds, narrowing of the candidate region to the sequences flanked by D3S1589 and D3S1541, and the finding in one family of a genomic DNA deletion whose centromeric end is located between these two flanking markers. 6 refs., 2 figs.

  14. 秀水街的昨天与今天

    Institute of Scientific and Technical Information of China (English)

    苏珊; 张佳敏

    2008-01-01

    Old Silk Street老秀水The former outdoor Silk Street (xiushuijie) was not just another tourist destination,it was also a place of really good stories to impress the people back home.The tall tales usually had elements of extremely harsh weather conditions,such as blistering cold temperatures,or scorching heat,given that both Beijing's"Spring"and"Autumn"seasons only lasts a few short weeks.Also, in the tall tales,there would probably be one or two"bargaining battles."A typical story might be:"She wanted 480 y...

  15. Preparation of naphtalene monocrystals for scintillometers; Preparacion de monocristales de naftaleno para contadores de centelleo

    Energy Technology Data Exchange (ETDEWEB)

    Gaeta, R.

    1959-07-01

    A method to prepare naphthalene crystals for scintillometers is described. The different methods of preparation and the reasons to choose the Bridgman method are analysed. The thermal curves of the growing furnaces, blister shape, use of curved capillaries and the most convenient growing velocities are studied in detail. Methods of preparation of high purity naphthalene by means of chromatography and zone melting are described. Finally the operations of cutting and polishing of crystal and their most significant physics properties are studied. (Author) 28 refs.

  16. 读书

    Institute of Scientific and Technical Information of China (English)

    2007-01-01

    1 Extremes along the Silk Road:Adventures off the World s Oldest Superhighway《极限丝绸之路》Author:Nick Middleton Publisher:John Murray Groundbreaking traveler and TV personality Nick Middleton follows in the footsteps of Alexander the Great and Marco Polo across one of the least hospitable regions on Earth—a succession of hostile deserts and towering mountain ranges,a harsh terrain of howling winds,searing heat and blistering cold—to encounter those who eke out existences there today.Personal experiences...

  17. Staphylococcus hyicus exfoliative toxins selectively digest porcine desmoglein 1

    DEFF Research Database (Denmark)

    Fudaba, Y.; Nishifuji, K.; Andresen, Lars Ole;

    2005-01-01

    Virulent strains of Staphylococcus hyicus can cause exudative epidermitis in pigs. The major symptom of this disease is exfoliation of the skin in the upper stratum spinosum. Exfoliation of the skin is strongly associated with exfoliative toxin including ExhA, ExhB, ExhC, ExhD, SHETA, and SHETB...... of Exhs in porcine skin. Each of recombinant Exhs injected in the skin of pigs caused superficial epidermal blisters or crust formation. Cell surface staining of desmoglein 1, but not that of desmoglein 3, was abolished when cryosections of normal porcine skin were incubated with one of Exhs suggesting...

  18. A multiple drug allergy syndrome, multiple drug intolerance syndrome and/or allergic drug reaction with multiple immune reactants

    Directory of Open Access Journals (Sweden)

    Ana Maria Abreu Velez

    2016-01-01

    Full Text Available Background: Adverse drug reactions (ADR, multiple drug allergy syndrome (MDAS and multiple drug intolerance syndrome (MDIS, are very common in the clinical practice worldwide due to an aging population. Case report: Here we describe a 61 year old Caucasian female, who presented complaining of large, tense bullae on her right anterior arm; the bullae had been present for one week, with pruritus. The patient was taking multiple medications. The patient presented with multiple large, tense blisters and scattered, smaller blisters on her legs and axillae. Materials and Methods: Biopsies for hematoxylin and eosin (H&E examination, direct immunofluorescence (DIF and immunohistochemistry (IHC analysis were performed. The H&E staining demonstrated diffuse, moderate epidermal spongiosis, with a subepidermal blister. Lymphocytes and eosinophils were present within the blister lumen and around superficial dermal blood vessels, hair follicles and sweat glands. Notably, blood vessels and lymphatics had altered shapes, many being narrowed, twisted and or dilated. DIF showed significant deposits of fibrinogen, Complement/C3c and IgA around the dermal vessels; some reactivity was also present at the basement membrane zone of the skin, as well as around dermal skin appendices. Of great interest was the reactivity seen with anti-human fibrinogen against some dermal cell junctions. IHC staining showed the presence of mainly CD4 and BCL-2 positive cells around skin appendices, with a few CD8 positive cells also present. Cyclooxygenase-2 was also very positive around the dermal blood vessels, as well as within cells of the dermal inflammatory infiltrate. The dermal lymphatics and dermal blood vessels appeared dilated, demonstrated by staining for D2-40/podoplanin and von Willembrand factor. Conclusion: Adverse drug reactions and multiple drug allergy syndrome have several overlapping features; the spectrum of immunopathologic and histopathologic features are not

  19. Advanced eddy current technique for measurement of annular gap between pressure tube and calandria tube in Indian Pressurized Heavy Water Reactors (PHWRs)

    International Nuclear Information System (INIS)

    In Indian Pressurised Heavy Water Reactors (PHWRs), the PT (pressure tube) is designed to be nominally concentric with the encircling CT (calandria tube). Due to various factors PT becomes eccentric with respect to CT over the life of reactor. If this becomes excessive, hot PT will come in contact with cold CT. Such a cold spot could act as potential location for initiating blister formation and premature failure of PT. Hence it is important to periodically measure annular gap between PT and CT. An advanced eddy current technique has been successfully developed and incorporated in BARCIS (BARC Channel Inspection System) for measurement of PT-CT gap. (author). 4 refs., 3 figs

  20. The effects of selenium and other micronutrients on the antioxidant activities and yield of corn (Zea mays L.) under drought stress

    OpenAIRE

    Sajedi, Nour Ali; Ardakani, Mohammad Reza; Madani, hamid; Naderi, Ahmad; Miransari, Mohammad

    2011-01-01

    The effects of selenium (Se) on plant growth under drought stress and in the presence of micronutrients are yet to be investigated. Hence, in a field experiment in 2007 the effects of Se and micronutrients including iron (Fe), zinc (Zn), copper (Cu), manganese (Mn), boron (B) and molybdenum (Mo) were evaluated on corn (Zea mays L.) grain yield under drought stress. Main- and sub-plots were devoted to irrigation (control and water stressed at the eight-leaf, blister and grain filling stages) a...

  1. Pemphigus vulgaris: a rare case report

    Directory of Open Access Journals (Sweden)

    Hari Babu Ramineni

    2015-06-01

    Full Text Available Pemphigus Vulgaris (PV is an organ-specific autoimmune disorder affecting skin and mucous membranes with a characteristic of intraepithelial blistering. The first common sign of this disease is involvement of oral mucosa followed by skin involvement. We here in report a rare case one such recently seen by us, where oral lesions and skin lesions in a 24 year old female patient presenting with a five months history of multiple fluid filled lesions all over the body, who is known case of psychosis finally diagnosed as having pemphigus vulgaris. [Int J Res Med Sci 2015; 3(6.000: 1543-1544

  2. Erythema multiforme following application of hair dye

    Directory of Open Access Journals (Sweden)

    Sankha Koley

    2012-01-01

    Full Text Available Erythema multiforme (EM is an acute mucocutaneous hypersensitivity reaction with varying degrees of blistering and ulceration. Common causes of EM are herpes simplex virus infection, mycoplasma infection, drug hypersensitivity, vaccination and drug-virus interaction. EM induced by contact dermatitis is rare. Paraphenylene diamine, a common ingredient in many hair dyes, is well known to produce allergic contact dermatitis. We report a 35-year-old lady presenting with EM following severe contact dermatitis to hair dye. So far as we know, this is the first report from India describing EM following contact dermatitis.

  3. Epidermolytic ichthyosis in a dog: clinical, histopathological, immunohistochemical and ultrastructural findings.

    Science.gov (United States)

    Mecklenburg, L; Hetzel, U; Ueberschär, S

    2000-05-01

    Epidermolytic hyperkeratosis (EH) in man is a complex of congenital, ichthyosiform skin diseases characterized clinically by blistering and hyperkeratosis. These clinical signs are the result of a collapse of the cytoskeleton, seen ultrastructurally as tonofilament clumping and cytolysis within terminally differentiating epidermal cells. In man, specific mutations in keratin 1, 2e, 9 or 10 underlie the various types of EH. This report describes the clinical, histopathological, immunohistochemical and ultrastructural findings in a 6-month-old dog with severe multifocal hyperkeratosis. The morphological changes were comparable with those of EH in man, indicating that this disease, presumably with a similar underlying pathogenetic mechanism, also occurs in the dog. PMID:10805985

  4. Progress and opportunities for tissue-engineered skin

    Science.gov (United States)

    MacNeil, Sheila

    2007-02-01

    Tissue-engineered skin is now a reality. For patients with extensive full-thickness burns, laboratory expansion of skin cells to achieve barrier function can make the difference between life and death, and it was this acute need that drove the initiation of tissue engineering in the 1980s. A much larger group of patients have ulcers resistant to conventional healing, and treatments using cultured skin cells have been devised to restart the wound-healing process. In the laboratory, the use of tissue-engineered skin provides insight into the behaviour of skin cells in healthy skin and in diseases such as vitiligo, melanoma, psoriasis and blistering disorders.

  5. Vitiligo after Diphencyprone for Alopecia Areata

    Directory of Open Access Journals (Sweden)

    Mario Cezar Pires

    2010-01-01

    Full Text Available The topical immunotherapy is used to treat alopecia areata and recalcitrant warts since the 1970s. Diphencyprone is a contact sensitizer used to treat dermatological conditions resulting from as altered immunological state, such as extensive alopecia areata, being partially effective and safe. Side effects include local eczema with blistering, regional lymphadenopathy and contact urticaria. Rare adverse effects include an erythema multiforme-like reaction, hyperpigmenttion, hypopigmentation, and vitiligo. We report a 30-year-old, Brazilian male who developed vitiligo lesions following DPCP therapy for alopecia areata.

  6. Quantification of pizza baking properties of different cheeses, and their correlation with cheese functionality.

    Science.gov (United States)

    Ma, Xixiu; Balaban, Murat O; Zhang, Lu; Emanuelsson-Patterson, Emma A C; James, Bryony

    2014-08-01

    The aim of this study is to quantify the pizza baking properties and performance of different cheeses, including the browning and blistering, and to investigate the correlation to cheese properties (rheology, free oil, transition temperature, and water activity). The color, and color uniformity, of different cheeses (Mozzarella, Cheddar, Colby, Edam, Emmental, Gruyere, and Provolone) were quantified, using a machine vision system and image analysis techniques. The correlations between cheese appearance and attributes were also evaluated, to find that cheese properties including elasticity, free oil, and transition temperature influence the color uniformity of cheeses. PMID:25048865

  7. Nevirapine-induced Stevens-Johnson syndrome from a tertiary care hospital in Central India

    Directory of Open Access Journals (Sweden)

    Vandana Badar

    2014-04-01

    Full Text Available A 42 year old man, a known case of AIDS receiving antiretroviral therapy – stavudine, lamivudine, and nevirapine since 4 weeks, was admitted with erythematous rash, with blisters, and abnormal liver function test. A diagnosis of Steven-Johnson syndrome was made excluding other opportunistic infection and differential diagnosis. Nevirapine was discontinued until the patient recovered. After recovery patient was started with stavudine, lamivudine, and efavirenz treatment without re-challenge with nevirapine. [Int J Basic Clin Pharmacol 2014; 3(2.000: 408-409

  8. Composition, structure and properties of POPC–triolein mixtures. Evidence of triglyceride domains in phospholipid bilayers

    DEFF Research Database (Denmark)

    Duelund, Lars; Jensen, Grethe Vestergaard; Hannibal-Bach, Hans Kristian;

    2013-01-01

    We have in this study investigated the composition, structure and spectroscopical properties of multilamellar vesicles composed of a phospholipid, 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC), and up to 10mol% of triolein (TO), a triglyceride. We found in agreement with previous results......% TO contained pure TO domains. These observations are consistent with an earlier MD simulation study by us and our co-workers suggesting triglycerides to be located in lens shaped, blister-like domains between the two lipid bilayer leaflets (Khandelia et al. (2010) [26])....

  9. Crescendo response to rituximab in oral pemphigus vulgaris: a case with 7-year follow-up.

    Science.gov (United States)

    Greenblatt, D T; Benton, E C; Groves, R W; Setterfield, J F

    2016-07-01

    Pemphigus vulgaris (PV) is an autoimmune blistering disease affecting the skin and mucous membranes. Rituximab, a CD20 chimeric monoclonal antibody, has efficacy in PV management. We report a case of severe oral PV that showed a progressive response to repeated courses of rituximab, culminating in a rapid response within 4 weeks following severe relapse 4 years after initial therapy. It demonstrates the progressively shorter time to achieve partial or complete remission following rituximab infusions, combined with minimal adjuvant therapy over a 7-year follow-up period.

  10. A Comparison of Human Neutrophils Acquired from Four Experimental Models of Inflammation

    Science.gov (United States)

    Motwani, Madhur P.; Day, Richard M.; Gilroy, Derek W.; O’Brien, Alastair J.

    2016-01-01

    Defects in neutrophil function have been implicated in a wide spectrum of clinical conditions. Several models are employed to study activated human neutrophils akin to those found at a site of inflammation. These include whole blood (WB) ex vivo stimulation with lipopolysaccharide (LPS) and in vivo techniques: cantharidin blister, skin windows and intra-dermal injection of UV-killed E.coli (UVKEc). Neutrophils obtained from these have never been compared. We compared the activation status of neutrophils from each technique in order to inform the optimal model for use in human studies. Healthy male volunteers were randomised to undergo one of the four techniques (n = 5/group). LPS: WB stimulated with 1ng/ml of LPS for 4 hours. Cantharidin: 12.5μl of 0.1% cantharidin elicited a single blister, aspirated at 24 hours. Skin windows: four 6mm mechanical-suction blisters created, de-roofed and an exudate-collection chamber placed over the windows for 4 hours before aspiration. UVKEc: 1.5 x 107 UVKEc injected intra-dermally. A single 10mm mechanical-suction blister formed and aspirated at 4 hours. Unstimulated WB used as the control. Flow cytometry was used to determine activation status using CD16, CD11b, CD54, CD62L and CD88. Functional status was assessed with a phagocytosis assay. The pattern of neutrophil activation was similar in all models. Neutrophil CD11b was elevated in all models, most markedly in UVKEc (p<0.0001), and CD54 was also elevated but only significant in the LPS model (p = 0.001). CD62L was significantly reduced in all 4 models (p<0.0001) and CD88 was also suppressed in all. There were no changes in CD16 in any model, neither was there any significant difference in the phagocytic capacity of the neutrophils. In summary, there are no significant differences in activation marker expression or phagocytic capacity in the neutrophils obtained from each technique. Therefore we believe whole blood stimulation is the best model in experimentally challenging

  11. Study on a new nano-dispersing agent and its effect for increasing suspension ability of alcohol-based coatings

    Institute of Scientific and Technical Information of China (English)

    2004-01-01

    A study for further improving the suspension rate of alcohol-based coatings using a new nano-dispersing agent has been presented in this paper. The results of experiments show that the new nano-dispersing agent should be used in combination with organic auxiliary agent which significantly increases the suspension effect of alcohol-based coatings. By the methods, the suspension rates of 99%, 98%, 96% and 94% can be achieved at 2h, 2h, 48h and 72h, respectively. These alcohol-based coatings have characters of higher strength, lower gas evolution, better brush ability and no blister after ignition.

  12. Helium thermodesorption from Ni polycrystalline samples irradiated by He+-ions up to 1x1017-1x1018 ion/cm2 doses

    International Nuclear Information System (INIS)

    The dependence of thermodesorption (TD) spectra on irradiation dose and TD peak nature in the samples of polycrystalline nickel irradiated at 300 K by He+ (20 keV) ions up to different doses in the 1x1017 - 1x1018 ion/cm2 range are investigated. Six peaks in the thermodesorption spectrum at 450, 800, 960, 1170, 1300 and 1530 K have been found. The correlation between TD peaks and arising surface structures is established. The nature of some peaks is bound with blister formation

  13. Warfarin-induced skin necrosis diagnosed on clinical grounds and treated with maggot debridement therapy.

    Science.gov (United States)

    Biscoe, Anna Louise; Bedlow, Alison

    2013-01-01

    A patient with a history of deep vein thrombosis presented with painful bruising and blistering on his left leg 7-10 days after warfarin treatment. A complicated 2-month treatment followed, where vasculitis was originally diagnosed from histological findings before the final diagnosis of warfarin-induced skin necrosis (WISN) was made on clinical grounds. Warfarin was stopped, reversed and low molecular weight heparin started but, the lesions had progressed to full thickness necrosis. This was originally treated with conventional surgical debridement before introducing maggot debridement therapy (MDT) in an effort to try to salvage the limb.

  14. The Complexity of Pain Management in Patients with Erythromelalgia.

    Science.gov (United States)

    Patel, Neha; Chen, Emily; Cucchiaro, Giovanni

    2015-11-01

    A 15-year-old girl diagnosed with erythromelalgia was admitted to the hospital with severe pain in her feet associated with burning, pruritus, erythema, and swelling. She had not responded to conventional management and received some relief only from cold bath immersions, which resulted in chronic blistering and multiple episodes of superinfection. After a successful trial of spinal cord stimulation, she had a permanent implantation procedure. The spinal cord stimulator relieved her pain and improved function but not the sensation of burning pain. However, this pain resolved after she started daily mexiletine. This case demonstrates that erythromelalgia sometimes can be managed successfully with a combination of pharmacologic and interventional procedures. PMID:26528699

  15. Health-related quality of life in epidermolysis bullosa: Validation of the Dutch QOLEB questionnaire and assessment in the Dutch population

    OpenAIRE

    Yuen, WY; Frew, JW; Veerman, K; Heuvel, van den, E.P.J.; Murrell, DF; Jonkman, MF

    2014-01-01

    Defining the health-related quality of life (HRQoL) in patients suffering from the heritable blistering disease epidermolysis bullosa (EB) is important in assessing the efficacy of new treatments. The quality of life in EB questionnaire (QOLEB) is an English 17-item EB-specific HRQoL measurement tool. The aim of this study was to develop a validated and reliable QOLEB in Dutch and assess the HRQoL in Dutch EB patients. The QOLEB was translated to Dutch according to protocol. Fifty-five adult ...

  16. Research on the Interaction of Hydrogen-Bond Acidic Polymer Sensitive Sensor Materials with Chemical Warfare Agents Simulants by Inverse Gas Chromatography

    Directory of Open Access Journals (Sweden)

    Liu Yang

    2015-06-01

    Full Text Available Hydrogen-bond acidic polymers are important high affinity materials sensitive to organophosphates in the chemical warfare agent sensor detection process. Interactions between the sensor sensitive materials and chemical warfare agent simulants were studied by inverse gas chromatography. Hydrogen bonded acidic polymers, i.e., BSP3, were prepared for micro-packed columns to examine the interaction. DMMP (a nerve gas simulant and 2-CEES (a blister agent simulant were used as probes. Chemical and physical parameters such as heats of absorption and Henry constants of the polymers to DMMP and 2-CEES were determined by inverse gas chromatography. Details concerning absorption performance are also discussed in this paper.

  17. Plastic pipe systems failure investigation and diagnosis

    CERN Document Server

    Farshad, Mehdi

    2011-01-01

    Industrial and domestic piping is increasingly made from various plastics and composites, and these materials withstand heavy use over long periods. They are, however, affected by environmental and other factors over time and can degrade, causing major problems within piping systems. Farshad's book deals with why plastic pipes and systems fail, and with how to investigate and diagnose such failures. Pipes may buckle, fracture, change in dimensions and colour, blister and delaminate, corrode through stress, be abraded and obstructed: all these cause problems and lead to loss of efficient operat

  18. Association of bullous pemphigoid with malignancy: A myth or reality?

    Directory of Open Access Journals (Sweden)

    Joycelin Fernandes

    2014-01-01

    Full Text Available Bullous pemphigoid (BP is an autoimmune sub-epidermal blistering disorder of the skin. The association of BP with internal malignancy has always been a matter of debate with no consensus reached despite many published case reports and clinical trials. However, we report a hitherto unreported association of BP with squamous cell carcinoma of the tongue, wherein the patient had a recalcitrant downhill course despite adequate treatment measures with control of skin lesions being achieved only following excision of the tumor, and relapse coinciding with detection of metastasis. Hence, given the clinical behavior, it is reasonable to speculate that the association of malignancy was more than co-incidental.

  19. Disease: H00644 [KEGG MEDICUS

    Lifescience Database Archive (English)

    Full Text Available H00644 Ectodermal dysplasia/skin fragility syndrome Ectodermal dysplasia/skin fragility... syndrome is a very rare genodermatosis that develops skin fragility with tearing and blisters and conge...945625 (gene, desription) McGrath JA, Mellerio JE Ectodermal dysplasia-skin fragility syndrome. Dermatol Cli...Garrod DR, Eady RA Mutations in the plakophilin 1 gene result in ectodermal dysplasia/skin fragility...n G, Fassihi H, Chan I, Paller AS, Surucu S, McGrath JA Ectodermal dysplasia-skin fragility syndrome resulti

  20. Quantification of pizza baking properties of different cheeses, and their correlation with cheese functionality.

    Science.gov (United States)

    Ma, Xixiu; Balaban, Murat O; Zhang, Lu; Emanuelsson-Patterson, Emma A C; James, Bryony

    2014-08-01

    The aim of this study is to quantify the pizza baking properties and performance of different cheeses, including the browning and blistering, and to investigate the correlation to cheese properties (rheology, free oil, transition temperature, and water activity). The color, and color uniformity, of different cheeses (Mozzarella, Cheddar, Colby, Edam, Emmental, Gruyere, and Provolone) were quantified, using a machine vision system and image analysis techniques. The correlations between cheese appearance and attributes were also evaluated, to find that cheese properties including elasticity, free oil, and transition temperature influence the color uniformity of cheeses.

  1. Powder metallurgy development at SRL

    International Nuclear Information System (INIS)

    The Savannah River Laboratory (SRL) is developing a powder metallurgy (P/M) process for manufacturing reactor-grade fuel tubes containing high wt % U3O8-Al cores clad with 8001 aluminum. The P/M cores are made by isostatic compaction. They are assembled in billets, outgassed, and hot-extruded using conventional coextrusion techniques. Cores have been compacted with up to 100% U3O8 and tubes extruded with 80 wt % oxide cores. Irradiation tests have been made using P/M core tubes in the Savannah River reactors. These tubes contained U3O8 concentrations up to 59 wt % and no significant swelling or blistering occurred. The tubes were irradiated to ∼ 40% burnup or 1.6x1021 fissions/cc of core. This report discusses both small-scale and production tests for high-density P/M fuel development. The purpose of the P/M development program at SRL is to: determine the maximum U3O8 content that can be fabricated into thin wall tubes, irradiate high-density tubes to high burnup and assess irradiation and dimensional stability, continue metal forming studies for extrusion and drawing, and evaluate hydrostatic extrusion and hydrostatically assisted drawing of P/M core tubes. Experimental results of testing the fuel assemblies performance so far indicate that: cores containing fine (-325 mesh) U3O8 and aluminum powders can be made practically free of high-density areas using the outlined P/M pre blending and sieving techniques. U3O8-Al cores can be isostatically compacted with up to 100 wt U3O8 and tubes successfully extruded with up to 80 wt oxide; fission gas blistering of U3O8-Al P/M tubes as indicated by the blister tests is a function of fissions/cc of U3O8 in the core; Decreasing the fission density of oxide increases the threshold temperature for blister formation; U3O8-Al P/M fuel tubes with up to 59 wt U3O8 have been successfully irradiated in SRP reactor to 1.6 x 1021 fissions/cc of core or 7 x 1020 fissions/cc of U3O8 small-scale metal forming tests sufficiently mock up

  2. Stabilnost amlodipin besilata i atenolola u jednoslojnim i dvoslojnim tabletama

    OpenAIRE

    ARYAL, SAJJAN; Škalko-Basnet, Nataša

    2008-01-01

    Tablete s amlodipinom i atenololom pripremljene su ili u obliku jednoslojne tablete (miješani matriks) ili kao dvoslojne tablete (lijekovi u zasebnim slojevima) koristeći slične pomoćne tvari i uvjete tabletiranja. Tablete su pakirane u dvije vrste pakiranja, aluminijske folije (strip) ili PVC (blister) i čuvane u uvjetima ubrzanog starenja. Stabilnost je određivana pomoću HPLC metode nakon 0, 1, 2, 3 i 4,5 mjeseci i izražena kao sadržaj intaktnog lijeka. Sadržaj atenolola nije se značajn...

  3. Bullous Pemphigoid in a Patient with Psoriasis Treated with Cyclos

    Directory of Open Access Journals (Sweden)

    Hande Arda Ulusal

    2010-10-01

    Full Text Available The concomitant occurrence of psoriasis and bullous pemphigoid has been described previously, but the pathogenic mechanism of this relationship is still unknown. We describe a 34-year-old woman with a 22-year history of plaque-type psoriasis. On the third day of hospitalization, disseminated tense bullae developed on her neck and upper extremities. Histologic examination of biopsy specimen obtained from a vesicular lesion demonstrated a subepidermal blister. Direct immunofluorescence revealed IgG and C3 deposition in a linear pattern at the basement membrane zone. Both diseases were successfully treated with cyclosporine. However, the coexistence of these conditions represents a difficult therapeutic challenge.

  4. Chroococcales em Esteiras Microbianas em Bolha do Brejo do Pau Fincado, Rio de Janeiro, Brasil.

    OpenAIRE

    Loreine Hermida da Silva e Silva; Frederico Alves dos Santos Lopes; Deise de Oliveira Delfino; Fabiane Feder

    2007-01-01

    Chroococcales in blister microbial mats at brejo do Pau Fincado, Rio de Janeiro, Brazil.Brejo do Pau Fincado (220 55’ 56,75” S e 420 16’ 54,7” W) is a natural natural coastal aquatical body. It is located at the Lagoa de Araruama complex, formed at least 7000 years ago. The local climate is characterized by low precipitation ratesand high evaporation rates; due to this, the lagoons tend to become ypersalyne. Microbial mats are organic-sedimentary structures composed mainly of cyanobacteria. W...

  5. Pachyonychia congenita with woolly hair in a ten month old infant

    Directory of Open Access Journals (Sweden)

    Ehsani Amirhushang

    2008-01-01

    Full Text Available A 10-month-old female presented with severe progressive wedge-shaped thickening and discoloration of all twenty nails. Further evaluations revealed palmoplantar keratoderma along with recurrent acral blisters causing residual crusted ulcers which were present during the past six months. Other findings included scalp kinky hair and dental caries. Past medical and family history had remarkable findings such as natal teeth and similar skin lesions in her older brother since his infancy. The patients′ clinical presentations and history are compatible with pachyonychia congenita presenting with concomitant features of both subtypes 1 and 2.

  6. [Evaluation of professional exposure to chloride vinyl monomer and vinyl idene chloride for a pharmaceutical packaging worker].

    Science.gov (United States)

    Dorsi, F; De Grandis, D; Laurelli, R; Narda, R; Pietrantonio, E; Scarlini, E; Soldati, P S

    2007-01-01

    The study was conducted by Judicial Policy investigations of Prosecution's Office. The event was connected by a professional founded suspicion disease of a pharmaceutical worker. First information coming from the Authority indicated a chloride vinyl monomer (CVM) exposure. We applied a chemical risk assessment method to estimate real professional exposure. The method was based on the productive cycle, physical and chemical and toxicological properties. The method combined to environmental data permitted to formulate etiological hypothesis. The worker during drugs packaging was exposed to CVM and vinylidene chloride (CVDM) caused by blister warming and by glue deposition. We explain the evaluations by which we could consider the pollutant different distribution in workplaces.

  7. Bullous Skin Diseases: Classical Types of Autoimmune Diseases

    Directory of Open Access Journals (Sweden)

    Jan Damoiseaux

    2013-01-01

    Full Text Available The prototypic bullous skin diseases, pemphigus vulgaris, pemphigus foliaceus, and bullous pemphigoid, are characterized by the blister formation in the skin and/or oral mucosa in combination with circulating and deposited autoantibodies reactive with (hemidesmosomes. Koch’s postulates, adapted for autoimmune diseases, were applied on these skin diseases. It appears that all adapted Koch’s postulates are fulfilled, and, therefore, these bullous skin diseases are to be considered classical autoimmune diseases within the wide and expanding spectrum of autoimmune diseases.

  8. Preliminary test results on tungsten tile with castellation structures in KSTAR

    Energy Technology Data Exchange (ETDEWEB)

    Hong, Suk-Ho, E-mail: sukhhong@nfri.re.kr [National Fusion Research Institute, 169-148 Gwahangno, Yusung-Gu, Daejeon (Korea, Republic of); Department of Electrical Engineering, HanYang University, Seoul (Korea, Republic of); Department of Nuclear Fusion and Plasma Science, University of Science and Technology, 169-148 Gwahangno, Yusung-Gu, Daejeon (Korea, Republic of); Bang, Eun-Nam [National Fusion Research Institute, 169-148 Gwahangno, Yusung-Gu, Daejeon (Korea, Republic of); Lim, Sun-Taek; Lee, Jin-Young [Department of Nuclear Engineering, Seoul National University, Seoul (Korea, Republic of); Yang, Seung Jae [Department of Materials Science and Engineering, Seoul National University, Seoul (Korea, Republic of); Litnovsky, A.; Hellwig, M. [Institut für Energie- und Klimaforschung–Plasmaphysik, Forschungszentrum Jülich GmbH, Association EURATOM-FZJ, Trilateral Euregio Cluster, D-52425 Jülich (Germany); Matveev, D. [Institut für Energie- und Klimaforschung–Plasmaphysik, Forschungszentrum Jülich GmbH, Association EURATOM-FZJ, Trilateral Euregio Cluster, D-52425 Jülich (Germany); Department of Applied Physics, Ghent University, Plateaustraat 22, B-9000 Ghent (Belgium); Komm, M. [Institute of Plasma Physics AS CR, v.v.i., Za Slovankou 3, 182 00 Prague 8 (Czech Republic); Berg, M. van den [Dutch Institute for Fundamental Energy Research (DIFFER), Association EURATOM-FOM, Trilateral Euregio Cluster, Postbus 1207, 3430BE Nieuwegein (Netherlands); Lho, Taehyeop [National Fusion Research Institute, 169-148 Gwahangno, Yusung-Gu, Daejeon (Korea, Republic of); Department of Electrical Engineering, HanYang University, Seoul (Korea, Republic of); Park, Chong Rae [Department of Materials Science and Engineering, Seoul National University, Seoul (Korea, Republic of); and others

    2014-10-15

    Highlights: • Tungsten tile with different castellation structures was exposed to KSTAR plasmas. • The contribution of ions and neutrals on deposition inside the gap was studied. • The shaped castellation shows smaller penetration depth of ions by a factor of 1/2. • Blister formation and cracking have occurred during plasma shots. - Abstract: A bulk tungsten tile with conventional and shaped castellation structures was exposed to various plasmas in KSTAR during 2012 campaign, in order to verify the functions of the shaped castellation designed for ITER divertor. The thermal response of the tile during the campaign was measured by thermocouples. The tungsten tile was collected after the campaign and the castellation structures were examined. The deposition inside the gap was studied to identify the contributions of ions and charge exchange neutrals. The shaped castellation shows a penetration depth of ions smaller than that of conventional one by a factor of ∼2, thus less deposition inside the gap which is consistent with the prediction made by SPICE2 code. It seems that blister formation and cracking have occurred during plasma shots.

  9. Progress towards genetic and pharmacological therapies for keratin genodermatoses: current perspective and future promise.

    Science.gov (United States)

    Chamcheu, Jean Christopher; Wood, Gary S; Siddiqui, Imtiaz A; Syed, Deeba N; Adhami, Vaqar M; Teng, Joyce M; Mukhtar, Hasan

    2012-07-01

    Hereditary keratin disorders of the skin and its appendages comprise a large group of clinically heterogeneous disfiguring blistering and ichthyotic diseases, primarily characterized by the loss of tissue integrity, blistering and hyperkeratosis in severely affected tissues. Pathogenic mutations in keratins cause these afflictions. Typically, these mutations in concert with characteristic features have formed the basis for improved disease diagnosis, prognosis and most recently therapy development. Examples include epidermolysis bullosa simplex, keratinopathic ichthyosis, pachyonychia congenita and several other tissue-specific hereditary keratinopathies. Understanding the molecular and genetic events underlying skin dysfunction has initiated alternative treatment approaches that may provide novel therapeutic opportunities for affected patients. Animal and in vitro disease modelling studies have shed more light on molecular pathogenesis, further defining the role of keratins in disease processes and promoting the translational development of new gene and pharmacological therapeutic strategies. Given that the molecular basis for these monogenic disorders is well established, gene therapy and drug discovery targeting pharmacological compounds with the ability to reinforce the compromised cytoskeleton may lead to promising new therapeutic strategies for treating hereditary keratinopathies. In this review, we will summarize and discuss recent advances in the preclinical and clinical modelling and development of gene, natural product, pharmacological and protein-based therapies for these disorders, highlighting the feasibility of new approaches for translational clinical therapy. PMID:22716242

  10. Gasemission and surface modifications during helium implantation on nickel surfaces

    International Nuclear Information System (INIS)

    Simultaneous measurements are reported of the helium reemission and the surface modifications of poly- and single crystal nickel surfaces during helium ion-implantation at energie between 20 keV-150 keV in the temperature range 250C-9500C. In addition the He depth-profiles have been measured using the nuclear reaction method 3H(d,α)H. The binding characteristics of He in nickel is studied by isochronous annealing of the samples after the implantation process and a subsequent measurement of the desorption spectra, and the surfaces are investigated by optical microscopy and scanning electron microscopy. For all implantation energies and impact temperatures together with the surface modifications a simultaneous increase in helium reemission is observed at a critical fluency value, which depends on temperature and implantation energy. A qualitative explanation of the empirical relation between reemission rate and blister formation rate is given on the basis of a lateral stress model for blister formation. (orig./GSCH)

  11. Exposure of Equal-Channel Angular Extruded Tungsten to Deuterium Plasma

    Science.gov (United States)

    Liu, Feng; Xu, Yuping; Zhou, Haishan; Zhao, Sixiang; Li, Bo; Lyu, Guanghong; Yuan, Yue; Hao, Ting; Luo, Guangnan

    2015-07-01

    Surface morphology and deuterium retention in ultrafine-grained tungsten fabricated by equal-channel angular pressing (ECAP) have been examined after exposure to a low energy, high-flux deuterium (D) plasma at fluences of 3×1024 D/m2 and 1×1025 D/m2 in a temperature range of 100°C-150°C. The methods used were scanning electron microscopy (SEM) and thermal desorption spectroscopy (TDS). Sparse and small blisters (∼0.1 μm) were observed by SEM after D plasma irradiation on every irradiated surface; yet they did not exhibit significant structure or plasma fluence dependence. Larger blisters or protrusions appeared after subsequent TDS heating up to 1000°C. The TDS results showed a single D desorption peak at ∼220°C for all samples and the D retention increased with increasing numbers of extrusion passes, i.e., the decrease of grain sizes. The increased D retention in this low temperature range should be attributed to the faster diffusion of D along the larger volume fraction of grain boundaries introduced by ECAP. supported by the National Magnetic Confinement Fusion Science Program of China (Nos. 2013GB105001, 2013GB105002, 2015GB109001), National Natural Science Foundation of China (Nos. 11305213, 11405201), Technological Development Grant of Hefei Science Center of CAS (No. 2014TDG-HSC003), and China National Funds for Distinguished Young Scientists (No. 51325103)

  12. Epidermólisis ampollosa de la unión: implicación oral. A propósito de un caso Junctional epidermolysis bullosa: oral implication. A case report

    Directory of Open Access Journals (Sweden)

    A. Pipa Vallejo

    2010-04-01

    Full Text Available Se entiende por epidermólisis ampollosa un grupo de dermatosis no inflamatorias, mecano ampollosas, enfermedades clínicamente similares con afectación cutáneo mucosa, que tienen carácter hereditario, en general, y que se caracterizan por una excesiva fragilidad de la piel y mucosas ante pequeños o mínimos traumas mecánicos, dando lugar a la aparición de vesículas, ampollas, erosiones y escaras. Existe una gran variabilidad fenotípica y considerable morbimortalidad.Epidermolysis bullosa is the name given to a group of rare genetically determined diseases of de skin and mucosal membranes, characterized by the development of blisters and vesicles in response to minor trauma. Is characterized by extensive phenotypic variability with considerable morbidity and mortality. Epidermolysis bullosa is classified into distinct subtypes depending on the location of blistering within the cutaneous dermoepidermal basement membrane zone.

  13. Dynamics of endogenous phytohormone in broad bean leaf infected by Olpidium Viciae%巢豆油壶菌与蚕豆相互作用下植物内源激素的动态

    Institute of Scientific and Technical Information of China (English)

    严吉明; 叶华智

    2013-01-01

    To understand the physiological mechanism of induced histopathology and symptom of broad bean blister caused by Olpidium Viciae,the endogenous phytohormone levels of Zeatin (ZT),Indoleacetic acid (IAA),Gibberellin (GA),Cytokinins (KT) and Abscisic acid (ABA) in diseased and healthy tissues of leaves were determined by High Performance Liquid Chromatography (HLPC).The results showed that the levels of five endogenous hormones were higher in diseased tissues than in healthy tissues.At the early period of symptom development,a large increase in level of five hormones in the diseased tissue was detected along with active the cell division.At the late period of symptom development,the cell division in tumor tissues gradually stopped with the exception of a continuous increase in the rate of GA in tumor tissue.The increase rate of other hormones in tumor tissues decreased obviously but the contents of those hormones were still higher in diseased tissues than in healthy tissues.Changes in level of five endogenous hormones in diseased tissues reflected physiological mechanism of inducing histopathological change and symptom of broad bean blister.

  14. Cold hardiness of interspecific hybrids between Pinus strobus and P. wallichiana measured by post-freezing needle electrolyte leakage.

    Science.gov (United States)

    Lu, Pengxin; Colombo, Stephen J; Sinclair, Robert W

    2007-02-01

    Interspecific hybrids between eastern white pine (Pinus strobus L.) and Himalayan blue pine (P. wallichiana A. B. Jacks.) were developed in Ontario, Canada, to introduce blister rust (Cronartium ribicola Fisch.) resistance genes to P. strobus. There is concern that introducing blister rust resistance has resulted in reduced cold hardiness of the progeny compared with non-hybridized eastern white pine. To test the efficacy of backcrossing with P. strobus to improve cold hardiness, 1-year-old seedlings from hybrid crosses differing in P. strobus genome composition were artificially freeze-tested. In Experiment 1, unhardened seedlings were allowed to acclimate to progressively lower temperatures in a growth room, whereas in Experiment 2, seedlings were hardened outdoors under natural weather conditions in Sault Ste Marie, Ontario. Needle cold injury was determined by calculating relative electrical conductivity based on post-freezing electrolyte leakage. Results indicated that needle fascicles from unhardened seedlings of all genotypes in the greenhouse tolerated -5 degrees C for 3 hours with little or no injury. Cold hardiness increased in parallel with declining growth room minimum temperature over the 7-week period of hardening. Cold hardiness was improved for hybrid crosses with increased Pinus strobus genome composition in Experiment 2, but the results were less conclusive in Experiment 1. PMID:17241966

  15. Microscopic Deformation of Tungsten Surfaces by High Energy and High Flux Helium/Hydrogen Particle Bombardment with Short Pulses

    Science.gov (United States)

    Tokitani, Masayuki; Yoshida, Naoaki; Tokunaga, Kazutoshi; Sakakita, Hajime; Kiyama, Satoru; Koguchi, Haruhisa; Hirano, Yoichi; Masuzaki, Suguru

    The neutral beam injection facility in the National Institute of Advanced Industrial Science and Technology was used to irradiate a polycrystalline tungsten specimen with high energy and high flux helium and hydrogen particles. The incidence energy and flux of the beam shot were 25 keV and 8.8 × 1022 particles/m2 s, respectively. The duration of each shot was approximately 30 ms, with 6 min intervals between each shot. Surface temperatures over 1800 K were attained. In the two cases of helium irradiation, total fluence of either 1.5 × 1022 He/m2 or 4.0 × 1022 He/m2 was selected. In the former case, large sized blisters with diameter of 500 nm were densely observed. While, the latter case, the blisters were disappeared and fine nanobranch structures appeared instead. Cross-sectional observations using a transmission electron microscope (TEM) with the focused ion beam (FIB) technique were performed. According to the TEM image, after irradiation with a beam shot of total fluence 4.0 × 1022 He/m2 , there were very dense fine helium bubbles in the tungsten of sizes 1-50 nm. As the helium bubbles grew the density of the tungsten matrix drastically decreased as a result of void swelling. These effects were not seen in hydrogen irradiation case.

  16. Microstructural development and helium bubble formation in Cu/W(Re) nanometer multilayer films irradiated by He{sup +} ion

    Energy Technology Data Exchange (ETDEWEB)

    Chen, Shunli [Key Laboratory of Radiation Physics and Technology (Sichuan University), Ministry of Education, Institute of Nuclear Science and Technology, Sichuan University, Chengdu 610064 (China); Liu, Bo, E-mail: liubo2009@scu.edu.cn [Key Laboratory of Radiation Physics and Technology (Sichuan University), Ministry of Education, Institute of Nuclear Science and Technology, Sichuan University, Chengdu 610064 (China); Lin, Liwei, E-mail: linliwei@scu.edu.cn [Key Laboratory of Radiation Physics and Technology (Sichuan University), Ministry of Education, Institute of Nuclear Science and Technology, Sichuan University, Chengdu 610064 (China); Jiao, Guohua [Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055 (China)

    2015-07-01

    A series of 60 keV He{sup +} implantations was conducted on Cu/W(Re, 5.9 at.%) multilayered structures with ion doses from 5 × 10{sup 19} to 5 × 10{sup 21} m{sup 2} under different temperature. Three distinct, temperature-dependent He release mechanisms were found by subsequent X-ray diffraction (XRD) and scanning electron microscope (SEM) investigations. Firstly, with implantation at 300 K (about T/T{sub m} {sub (Cu)} = 0.22), a certain degree of blistering was observed with a critical dose higher than 5 × 10{sup 21} m{sup −2}. But, at higher temperature irradiation (about T/T{sub m} {sub (Cu)} = 0.35), samples implanted were characterized by extensive blisters at the dose of 2 × 10{sup 21} m{sup −2}. Finally, at 673 K (about T/T{sub m} {sub (Cu)} = 0.5), the specimen flaked and a rough, porous surface formed when the dose was higher than 1 × 10{sup 21} m{sup −2}. The mechanisms involved have been analyzed based on the detailed characterization studies.

  17. Microstructural development and helium bubble formation in Cu/W(Re) nanometer multilayer films irradiated by He+ ion

    International Nuclear Information System (INIS)

    A series of 60 keV He+ implantations was conducted on Cu/W(Re, 5.9 at.%) multilayered structures with ion doses from 5 × 1019 to 5 × 1021 m2 under different temperature. Three distinct, temperature-dependent He release mechanisms were found by subsequent X-ray diffraction (XRD) and scanning electron microscope (SEM) investigations. Firstly, with implantation at 300 K (about T/Tm (Cu) = 0.22), a certain degree of blistering was observed with a critical dose higher than 5 × 1021 m−2. But, at higher temperature irradiation (about T/Tm (Cu) = 0.35), samples implanted were characterized by extensive blisters at the dose of 2 × 1021 m−2. Finally, at 673 K (about T/Tm (Cu) = 0.5), the specimen flaked and a rough, porous surface formed when the dose was higher than 1 × 1021 m−2. The mechanisms involved have been analyzed based on the detailed characterization studies

  18. Microstructure and Anticorrosion Property of AT13 Coatings Made by Combination of Nanoparticles Doping and Plasma Spraying Technique

    Institute of Scientific and Technical Information of China (English)

    LI Chun-fu; DAI Jia-lin; WANG Bin; ZHANG Ying; HE Tao-e; LUO Ping-ya

    2004-01-01

    Al2O3+13wt%TiO2 (AT13) particles were doped with 5%~30% nanoparticles and prefabricating powders were prepared by renewed granulation. AT13 coatings were prepared on the surface of steel 45# by air plasma spraying technique with the prefabricating powders. The microstructures of the AT13 prefabricating powders and the resulting coatings were investigated by SEM and EDS and XRD. The samples were undergone corrosion in the medium of 10% H2SO4 aqueous solution at temperature 80℃. The results indicate that the blistering time of coatings in the corrosive medium was increased with the increase of doped nanoparticle concentration while the time from blistering to spalling is independent of nanoparticle concentration. The results revealed that the structure of prefabricating powders was a twisted micrometer grade particle with dimension of 40-60μm, encapsulated by nanoparticles. The homogeneity of element distribution in coatings was improved and porosity was reduced. The phases of (Al2O3) 5.333 and orthorhombic Al2TiO5 were identified . The fracture analysis confirmed that there is a large amount of vermiculate whiskers with diameter of 10nm and length of 100~200 nm in coatings and the fracture type of coatings was the ductile trans-granular fracture.

  19. Case of superficial mucocele of the lower lip.

    Science.gov (United States)

    Oka, Masahiro; Nishioka, Eri; Miyachi, Rieko; Terashima, Mina; Nishigori, Chikako

    2007-11-01

    Superficial mucocele is a relatively rare bullous disease that develops in the oral mucosa. Although the number of reported cases is limited, it seems that the superficial mucocele has been recognized as an independent disease belonging to a single entity. We report a 48-year-old woman who repeatedly developed superficial mucocele in the oral mucosa. When she was admitted to our hospital she had a tense vesicle on her lower lip. A skin biopsy was taken from the vesicle. A blister containing neutrophils and erythrocytes was present in the mucous epithelium. There was a dermal infiltrate comprising neutrophils, mononuclear lymphocytic cells and eosinophils. Dilated salivary gland excretory ducts were seen in the lamina propria mucosae. Eosinophilic and amorphous materials, which were periodic acid-Schiff positive after digestion with diastase, and alcian blue positive, were deposited in the blister. Direct immunofluorescence was negative for immunoglobulin (Ig)G, IgM, IgA, C1q, C3 and C4. These histological findings led us to make a diagnosis of superficial mucoceles. PMID:17973814

  20. Simple Instrumental and Visual Tests for Nonlaboratory Environmental Control

    Directory of Open Access Journals (Sweden)

    L. P. Eksperiandova

    2016-01-01

    Full Text Available Proposed are simple and available techniques that can be used for rapid and reliable environmental control specifically of natural water by means of instrumental and visual tests in outdoor conditions. Developed are the chemical colorimetric modes for fast detection of socially dangerous trace impurities in water such as Co(II, Pd(II, and Rh(III as well as NO2--ions and Fe(III serving as model impurities. Application of portable digital devices and scanner allows estimating the color coordinates and increasing the accuracy and sensitivity of the tests. The combination of complex formation with preconcentration of colored complexes replaces the sensitive but time-consuming and capricious kinetic method that is usually used for this purpose at the more convenient and reliable colorimetric method. As the test tools, the following ones are worked out: polyurethane foam tablets with sorbed colored complexes, the two-layer paper sandwich packaged in slide adapter and saturated by reagents, and polyethylene terephthalate blister with dried reagents. Fast analysis of polyurethane foam tablets is realized using a pocket digital RGB-colorimeter or portable photometer. Express analysis of two-layer paper sandwich or polyethylene terephthalate blister is realized by visual and instrumental tests. The metrological characteristics of the developed visual and instrumental express analysis techniques are estimated.

  1. Occurrence and research progress of fruit tree viroid diseases in China%中国果树类病毒的发生及其研究进展

    Institute of Scientific and Technical Information of China (English)

    王国平; 洪霓; Ahmed Hadidi

    2005-01-01

    概述了在中国发生且己鉴定明确的5种果树类病毒,即苹果绣果类病毒(Apple scar skin viroid,ASSVd)、梨泡状溃疡类病毒(Pear blister canker viroid,PBCVd)、葡萄黄斑类病毒-1(Grapevine yellow speckle viroid-1,GYSVd-1)、柑橘裂皮类病毒(Citrus exocortis viroid,CEVd)和桃潜隐花叶类病毒(Peach latent mosaic viroid,PLMVd)的研究进展,包括病害的首次发现、症状特征、发病规律、检测方法与防治对策以及这些类病毒的生物学与分子生物学特性.%This paper has briefly reviewed the research progress on five fruit tree viroids reported in China. The viroids are ASSVd causing scar skin disease on apple, PBCVd causing blister canker on pear,GYSVd-1 causing yellow speckle on grapevine, CEVd causing exocortis on citrus and PLMVd causing latent mosaic on peach. It includes the discovery and symptoms of these viroid diseases in China, biochemical and molecular properties, occurrence and detection techniques of these viroids.

  2. The effect of aerobic corrosion on anaerobically-formed sulfide layers on carbon steel in dilute near-neutral pH saline solutions

    International Nuclear Information System (INIS)

    Highlights: •The corrosion rate is low when steel is exposed to anaerobic conditions (pH = 8.9). •An anaerobic corrosion with sulfide to aerobic switch increases the corrosion rate. •Aerobic conditions leads to corrosion and oxide deposition beneath FeS. •Continual air exposure leads to the blistering of the original FeS film. -- Abstract: The aerobic corrosion of pipeline steel was investigated in an aqueous sulfide solution by monitoring the corrosion potential and periodically measuring the polarization resistance. The properties and composition of the corrosion product deposits formed were determined using scanning electron microscopy, energy dispersive X-ray analysis, and Raman spectroscopy. The establishment of aerobic conditions leads to corrosion and (oxyhydr)oxide deposition beneath the anaerobically-formed mackinawite film originally present on the steel surface. This leads to blistering and spalling of the sulfide film. Chemical conversion of the mackinawite to Fe(III) (oxyhydr)oxides also occurs but is a relatively slow reaction

  3. Optimizing resistance spot welding parameters for vibration damping steel sheets

    Energy Technology Data Exchange (ETDEWEB)

    Oberle, H. [Centre de Recherches et Developpements Metallurgiques, Sollac (France); Commaret, C.; Minier, C. [Automobiles Citroeen PSA (France); Magnaud, R. [Direction des Methodes Carrosserie, Renault (France); Pradere, G. [Materials Engineering Dept., Renault (France)

    1998-01-01

    In order to meet the growing demand for functionality and comfort in vehicles, weight and quietness are major concerns for carmakers and materials suppliers. Noise reduction by damping vibrations can meet both aspects. Therefore, steelmakers have developed vibration damping steel sheets (VDSS), which are a three-layer composite material composed of two steel sheets sandwiching a viscoelastic resin core. Industrial use of VDSS in automobiles usually implies the product can be resistance welded. The intent of this investigation is to set up rules to optimize resistance spot welding of VDSS. Two phenomena are the focus of this research: the reduction of blistering and gas expulsion holes. Different aspects are studied, such as the effect of polymer presence and of electrode shape on welding domain and the evaluation of the influence of a welding schedule on blistering and expulsion holes. It appears that polymer presence has no effect on domain width, but does on its position. Higher frequency of expulsion holes with truncated electrodes can be explained with mechanical considerations. From the influence of short circuit voltage, current delay angle and welding schedule on the frequency of gas expulsion holes, a mechanism responsible for expulsion holes is proposed and optimal welding parameters are given.

  4. PEMPHIGUS: A DISEASE STAMPED IN THE SKIN

    Directory of Open Access Journals (Sweden)

    Aline Bicalho Matias

    2013-11-01

    Full Text Available Introduction: Pemphigus are autoimmune blistering diseases that affect the skin and mucous membranes. The blisters characteristics of pemphigus tend to break, causing painful erosions that easily bleed. This study aimed to identify the experiences involved in the triggering of pemphigus and how patients face the illness and treatment. Material and Methods: The study included 31 patients diagnosed with pemphigus foliaceus and vulgaris, under a standardized pulsetherapy treatment. Data collection was through semi-structured interviews, which were audio-recorded, transcribed and analyzed using a qualitative approach. Results: During the journey in search of the phenomenon, were defined two thematic categories, subdivided into eleven subcategories. Months before the triggering of the illness, patients experienced feelings of losses, familiar conflicts and concerns; also showed an experience permeated by heartache and disappointments. After the first signs of pemphigus, patients experienced a long journey until the correct diagnose; worsening of the lesions after the communication of the diagnosis; feelings of isolation, shame and prejudgment; interruptions of the future plans; lack of information about the disease and treatment; difficult adherence to the pulsetherapy and the appearance of new lesions or worse by stressful events. Discussion: It might be observed that the disease is not just a biological deviation, but also a social deviance, which explicit the need to adapt to the new reality of the disease and face the isolation, prejudgment and shame of living with a stigmatizing disease.

  5. Mustard vesicating agent-induced toxicity in the skin tissue and silibinin as a potential countermeasure.

    Science.gov (United States)

    Tewari-Singh, Neera; Agarwal, Rajesh

    2016-06-01

    Exposure to the vesicating agents sulfur mustard (SM) and nitrogen mustard (NM) causes severe skin injury with delayed blistering. Depending upon the dose and time of their exposure, edema and erythema develop into blisters, ulceration, necrosis, desquamation, and pigmentation changes, which persist weeks and even years after exposure. Research advances have generated data that have started to explain the probable mechanism of action of vesicant-induced skin toxicity; however, despite these advances, effective and targeted therapies are still deficient. This review highlights studies on two SM analogs, 2-chloroethyl ethyl sulfide (CEES) and NM, and CEES- and NM-induced skin injury mouse models that have substantially added to the knowledge on the complex pathways involved in mustard vesicating agent-induced skin injury. Furthermore, employing these mouse models, studies under the National Institutes of Health Countermeasures Against Chemical Threats program have identified the flavanone silibinin as a novel therapeutic intervention with the potential to be developed as an effective countermeasure against skin injury following exposure to mustard vesicating agents. PMID:27326543

  6. Complex Challenges of Maintaining Whitebark Pine in Greater Yellowstone under Climate Change: A Call for Innovative Research, Management, and Policy Approaches

    Directory of Open Access Journals (Sweden)

    Andrew Hansen

    2016-02-01

    Full Text Available Climate suitability is projected to decline for many subalpine species, raising questions about managing species under a deteriorating climate. Whitebark pine (WBP (Pinus albicaulis in the Greater Yellowstone Ecosystem (GYE crystalizes the challenges that natural resource managers of many high mountain ecosystems will likely face in the coming decades. We review the system of interactions among climate, competitors, fire, bark beetles, white pine blister rust (Cronartium ribicola, and seed dispersers that make WBP especially vulnerable to climate change. A well-formulated interagency management strategy has been developed for WBP, but it has only been implemented across <1% of the species GYE range. The challenges of complex climate effects and land allocation constraints on WBP management raises questions regarding the efficacy of restoration efforts for WBP in GYE. We evaluate six ecological mechanisms by which WBP may remain viable under climate change: climate microrefugia, climate tolerances, release from competition, favorable fire regimes, seed production prior to beetle-induced mortality, and blister-rust resistant trees. These mechanisms suggest that WBP viability may be higher than previously expected under climate change. Additional research is warranted on these mechanisms, which may provide a basis for increased management effectiveness. This review is used as a basis for deriving recommendations for other subalpine species threatened by climate change.

  7. Shell damage in the Tehuelche scallop Aequipecten tehuelchus caused by Polydora rickettsi (Polychaeta: Spionidae) infestation.

    Science.gov (United States)

    Diez, M E; Orensanz, J M; Márquez, F; Cremonte, F

    2013-10-01

    The different types of shell damage caused to the commercially valuable Tehuelche scallop (Aequipecten tehuelchus) by the polychaete Polydora rickettsi are described. X-rays, computerized tomography, shell sections, scanning electron microscopy, Energy Dispersive X-ray analysis (EDAX), mineralogical analyses and geometric morphometrics were applied to that end. Scallop shells presented three types of damage: (1) spots, (2) calcareous alterations, and (3) mud blisters. Microstructural alterations consisted of a simple conchiolin membranous layer in the case of spots, a series of interleaved layers of different degree of calcifications in calcareous alterations, and two different surface morphologies (muddy and mucous layers) in mud blisters. Damage was localized mainly along concentric growth rings, coincidentally with the location of most burrows, as shown by X-ray. Mineralogical analysis showed that in all cases (including non-infested shells) calcite was the calcium carbonate polymorph present. Geometric morphometrics showed that only 5% of shape variation was explained by infestation with P. rickettsi, irrespective of the type of damage. Number of worms per infested shell varied significantly among four beds. Left shells (upward-oriented) were significantly more affected than right shells, which are in closer contact with the bottom.

  8. Community Structure, Biodiversity, and Ecosystem Services in Treeline Whitebark Pine Communities: Potential Impacts from a Non-Native Pathogen

    Directory of Open Access Journals (Sweden)

    Diana F. Tomback

    2016-01-01

    Full Text Available Whitebark pine (Pinus albicaulis has the largest and most northerly distribution of any white pine (Subgenus Strobus in North America, encompassing 18° latitude and 21° longitude in western mountains. Within this broad range, however, whitebark pine occurs within a narrow elevational zone, including upper subalpine and treeline forests, and functions generally as an important keystone and foundation species. In the Rocky Mountains, whitebark pine facilitates the development of krummholz conifer communities in the alpine-treeline ecotone (ATE, and thus potentially provides capacity for critical ecosystem services such as snow retention and soil stabilization. The invasive, exotic pathogen Cronartium ribicola, which causes white pine blister rust, now occurs nearly rangewide in whitebark pine communities, to their northern limits. Here, we synthesize data from 10 studies to document geographic variation in structure, conifer species, and understory plants in whitebark pine treeline communities, and examine the potential role of these communities in snow retention and regulating downstream flows. Whitebark pine mortality is predicted to alter treeline community composition, structure, and function. Whitebark pine losses in the ATE may also alter response to climate warming. Efforts to restore whitebark pine have thus far been limited to subalpine communities, particularly through planting seedlings with potential blister rust resistance. We discuss whether restoration strategies might be appropriate for treeline communities.

  9. Microstructure and helium irradiation performance of high purity tungsten processed by cold rolling

    Science.gov (United States)

    Chen, Zhe; Han, Wenjia; Yu, Jiangang; Kecskes, Laszlo; Zhu, Kaigui; Wei, Qiuming

    2016-10-01

    This work aims to investigate the effects of confined cold rolling on the evolution of microstructure, hardness, and helium irradiation performance of high purity tungsten (W). Using a final rolling temperature of 450 °C, W samples were severely deformed by confined cold rolling up to equivalent strains (εeq) of 1.6 and 3.3. Experimental results indicate that the average grain size of W specimens processed by confined cold rolling has been greatly reduced, and the rolled W samples with εeq ∼3.3 do not show an "ideal texture" of (001)[110] which is the expected texture of bcc metals processed by conventional cold rolling. The irradiation resistance against 60 keV He+ ions with up to a dose of 1.5 × 1022 ions·m-2 of the rolled W is compared to that of the as-received W. Results show that, due to an improvement of the metal's ductility, blister bursting with a partially opened lid forms on the surface of the rolled W, whereas blister bursting with a fully opened lid forms on the surface of the as-received W.

  10. Treatment of Intraepidermal Autoimmune Bullous Diseases Sürekli Eğitim

    Directory of Open Access Journals (Sweden)

    Tamer İrfan Kaya

    2011-06-01

    Full Text Available Pemfigus is an autoimmune bullous skin disease, characterized by intraepidermal blisters. It is a severe and potentially life-threatening chronic disease with blisters and erosions on the mucosae and the skin. Treatment options do not differ for two most common types of pemphigus, pemphigus vulgaris and pemphigus foliaceus, except that the latter is usually less resistant to treatment and corticosteroids can often be started at lower doses. Systemic corticosteroids are still the most widely used drugs in the treatment of pemphigus and continue to be the mainstay of therapy for this disease. Adjuvant drugs are commonly used in combination with the aims of increasing efficacy and of having a steroid-sparing action, thereby allowing reduced corticosteroid side-effects. Mortality and complete remission rates have improved since the introduction of adjuvant drugs to pemphigus. Adjuvant drugs include immunoadsorbtion, corticosteroid pulse therapy, intravenous immunoglobulin (IVIG, immunosuppressive agents such as azathioprine, cyclophosphamide, mycophenolate mofetil and and anti-CD20 monoclonal antibody (rituximab. The lack of consensus in the published literature about the treatment of this disorder is responsible for different treatment strategies. Treatments need to be chosen after careful consideration of the potential benefits and side effects according to the patients’ medical condition. Here, both conventional therapies and novel treatment regimens for pemphigus are discussed. (Turkderm 2011; 45 Suppl 1: 44-53

  11. Batch fabrication of optical actuators using nanotube-elastomer composites towards refreshable Braille displays

    Science.gov (United States)

    Camargo, C. J.; Campanella, H.; Marshall, J. E.; Torras, N.; Zinoviev, K.; Terentjev, E. M.; Esteve, J.

    2012-07-01

    This paper reports an opto-actuable device fabricated using micro-machined silicon moulds. The actuating component of the device is made from a composite material containing carbon nanotubes (CNTs) embedded in a liquid crystal elastomer (LCE) matrix. We demonstrate the fabrication of a patterned LCE-CNT film by a combination of mechanical stretching and thermal cross-linking. The resulting poly-domain LCE-CNT film contains ‘blister-shaped’ mono-domain regions, which reversibly change their shape under light irradiation and hence can be used as dynamic Braille dots. We demonstrate that blisters with diameters of 1.0 and 1.5 mm, and wall thickness 300 µm, will mechanically contract under irradiation by a laser diode with optical power up to 60 mW. The magnitude of this contraction was up to 40 µm, which is more than 10% of their height in the ‘rest’ state. The stabilization time of the material is less than 6 s for both actuation and recovery. We also carried out preliminary tests on the repeatability of this photo-actuation process, observing no material or performance degradation. This manufacturing approach establishes a starting point for the design and fabrication of wide-area tactile actuators, which are promising candidates for the development of new Braille reading applications for the visually impaired.

  12. Deuterium retention and surface modifications of nanocrystalline tungsten films exposed to high-flux plasma

    Energy Technology Data Exchange (ETDEWEB)

    Hoen, M.H.J. ' t [FOM Institute DIFFER (Dutch Institute for Fundamental Energy Research), Association EURATOM-FOM, Partner in the Trilateral Euregio Cluster, 3439 MN Nieuwegein (Netherlands); Dellasega, D.; Pezzoli, A.; Passoni, M. [Politecnico di Milano, EURATOM-ENEA-CNR Association, Milano (Italy); Kleyn, A.W., E-mail: A.W.Kleijn@uva.nl [FOM Institute DIFFER (Dutch Institute for Fundamental Energy Research), Association EURATOM-FOM, Partner in the Trilateral Euregio Cluster, 3439 MN Nieuwegein (Netherlands); University of Amsterdam, Science Park 904, NL-1098 XH Amsterdam (Netherlands); Center of Interface Dynamics for Sustainability, CDCST, CAEP, Chengdu, Sichuan 610207 (China); Zeijlmans van Emmichoven, P.A. [FOM Institute DIFFER (Dutch Institute for Fundamental Energy Research), Association EURATOM-FOM, Partner in the Trilateral Euregio Cluster, 3439 MN Nieuwegein (Netherlands)

    2015-08-15

    Highlights: • The films withstand the intense plasma exposure maintaining overall integrity. • An increase of deuterium retention was observed with decreasing tungsten density. • Formation of micrometer-sized blisters as well as structures on the nanometer scale depending on the layer type. - Abstract: Deuterium retention studies are presented for nanostructured tungsten films exposed to high-flux deuterium plasmas. Thin tungsten films of ∼1 μm thickness were deposited with pulsed laser deposition (PLD) on bulk tungsten. Surface modifications were studied with scanning electron microscopy and deuterium retention with thermal desorption spectroscopy. Three types of PLD films with different densities and crystallinity were studied after exposure to deuterium plasmas. The surface temperature ranged from about 460 K at the periphery to about 520 K in the centre of the targets. The films withstand the intense plasma exposure well and maintain their overall integrity. An increase of deuterium retention is observed with decreasing tungsten density and crystallite size. We found that the filling of these thin films with deuterium is significantly faster than for pre-damaged polycrystalline tungsten. We observed formation of micrometer-sized blisters as well as structures on the nanometer scale, both depending on the layer type.

  13. CUSUM: A tool for early feedback about performance?

    Directory of Open Access Journals (Sweden)

    McLean Ian P

    2006-03-01

    Full Text Available Abstract Background Modern day clinical practice demands evidence justifying our choice of treatment methods. Cumulative sum techniques (cusum are amongst the simplest statistical methods known. They provide rapid analysis and identification of trends in a series of data. This study highlights use of these techniques as an early performance indicator of a clinical procedure before its implementation. Methods Twenty consecutive patients who underwent total hip or knee arthroplasty received a simple dressing – blue gauze and Tegaderm. Cusum charting was used to assess the dressing with regards to skin blistering. At an acceptable level of performance the curve would oscillate about the horizontal axis and the overall trend therefore said to be flat. If performance is unacceptable, the cusum slopes upward. Results The cusum plot for the twenty patients did not cross the specified control limits. This showed that our simple dressing met specified standards with regards to wound blistering postoperatively. Conclusion We recommend the use of this simple, yet versatile cusum technique in the early evaluation of a clinical procedure before its implementation.

  14. Radiation Stability of Triple Coatings Based on Transition-Metal Nitrides Under Irradiation By Alpha Particles and Argon Ions

    Science.gov (United States)

    Potekaev, A. I.; Kislitsyn, S. B.; Uglov, V. V.; Klopotov, A. A.; Gorlachev, I. D.; Klopotov, V. D.; Grinkevich, L. S.

    2016-05-01

    The data on the influence of irradiation of (Ti, Cr)N1-x coatings by helium and argon ions on their surface structure are presented. The (Ti, Cr)N1-x coatings 50-300 nm in thickness were formed on carbon steel substrates by vacuum-arc deposition. Irradiation of the coated specimens was performed in a DC-60 heavy-ion accelerator by low-energy 4He+1, 4He+2 and 40Ar5+ ions and high-energy 40Ar5+ ions up to the fluence 1.0·1017 ion/cm2 at the irradiation temperature not higher than 150°C. It is shown that irradiation of the (Ti, Cr)N1-x coating surface by 4He+1, 4He+2 and 40Ar5+ ions with the energy 20 keV/charge does not give rise to any noticeable structural changes nor any surface blistering, while its irradiation by 40Ar5+ ions with the energy 1.50 MeV/amu causes blistering.

  15. Efficacy and safety of artesunate plus amodiaquine in routine use for the treatment of uncomplicated malaria in Casamance, southern Sénégal

    Directory of Open Access Journals (Sweden)

    Vaillant Michel

    2007-11-01

    Full Text Available Abstract Background There are no data on the long term use of an artemisinin combination treatment in moderate or high transmission areas of Africa. Methods and findings Artesunate plus amodiaquine (AS+AQ was used to treat slide-proven Plasmodium falciparum-infected patients of all ages in the Oussouye district, Casamance, Senegal, over a period of six years (2000 to 2005. Efficacy, by Kaplan Meier survival analysis (n = 966, and safety (adverse event rates, n = 752 were determined over 28 days. A weight-based dosing regimen was used for the loose tablets during 2000–2003 (n = 731 and a commercially available co-blister was used during 2004–2005 (n = 235. Annual crude (non PCR corrected rates remained stable over the study period [range 88.5–96.7%; overall 94.6 (95% CI 92.9–95.9]. Nine co-blister treated patients (0.9% withdrew because of drug-related adverse events; seven had gastrointestinal complaints of whom two were hospitalized for vomiting. By Day 28, the mean total bilirubin (n = 72, AST (n = 94 and ALT (n = 95 values decreased. Three patients had Day 28 AST/ALT values > 40 Conclusion AS+AQ in combination was highly efficacious and well-tolerated in this area and justifies the decision to use it as first line treatment. Long-term monitoring of safety and efficacy should continue.

  16. Role of hydrocolloid dressings in the treatment of moist skin desquamation

    International Nuclear Information System (INIS)

    Moist skin desquamation has been of concern to radiation oncologists since the inception of radiation therapy as a major treatment modality. As treatment units became more sophisticated severe skin reactions became less of a problem, but never disappeared. In recent years, with the more aggressive use of chemotherapy concurrently with radiation therapy, and with some treatment regimens requiring high doses to the skin, moist desquamation has occurred more frequently. The principle of wound healing under occlusive moist conditions is based on the observation that untouched blisters showed faster reepithelialization than open blisters. As long ago as 1962, it was demonstrated that if tissue hydration were maintained the rate of epidermal resurfacing would be enhanced. The primary disadvantage has been the fear of infection. Since June 1984, the authors have treated 40 patients with moist desquamation with an occlusive hydrocolloid dressing. These patients had undergone radiation therapy for a variety of conditions, including Hodgkin disease, head and neck cancers, melanoma, and breast cancer. Some patients had received concurrent chemotherapy and experienced severe reactions. All patients healed well with good cosmesis, and there were no infections. Pain relief was excellent. This exhibit demonstrates the theory of moist occlusive healing. Dressing application and case history pictures illustrating the healing process are shown

  17. Dermal eosinophilic infiltrate in junctional epidermolysis bullosa.

    Science.gov (United States)

    Saraiya, Ami; Yang, Catherine S; Kim, Jinah; Bercovitch, Lionel; Robinson-Bostom, Leslie; Telang, Gladys

    2015-08-01

    Junctional epidermolysis bullosa (JEB) is a rare genodermatosis characterized by a split in the lamina lucida usually because of mutations in LAMA3, LAMB3 and LAMC2 resulting in absence or reduction of laminin-332. Rare subtypes of JEB have mutations in COL17A1, ITGB4, ITGA6 and ITGA3 leading to reduction or dysfunction of collagen XVII, integrin α6β4 and integrin α3. The classic finding under light microscopy is a paucicellular, subepidermal split. We describe the unusual presence of an eosinophilic infiltrate in the bullae and subjacent dermis in a neonate with JEB, generalized intermediate (formerly known as non-Herlitz-type JEB), discuss the histologic differential diagnosis for a subepidermal blister in a neonate, review the literature regarding cases of epidermolysis bullosa (EB) presenting with inflammatory infiltrates, and discuss mechanisms to explain these findings. This case highlights that eosinophils can rarely be seen in EB and should not mislead the dermatopathologist into diagnosing an autoimmune blistering disorder. PMID:25950805

  18. Maternal uniparental meroisodisomy in the LAMB3 region of chromosome 1 results in lethal junctional epidermolysis bullosa.

    Science.gov (United States)

    Takizawa, Y; Pulkkinen, L; Shimizu, H; Lin, L; Hagiwara, S; Nishikawa, T; Uitto, J

    1998-05-01

    Herlitz junctional epidermolysis bullosa (OMIM#226700) is a lethal, autosomal recessive blistering disorder caused by mutations in one of the three genes LAMA3, LAMB3, or LAMC2, encoding the constitutive polypeptide subunits of laminin 5. In this study, we describe a patient homozygous for a novel nonsense mutation Q936X in exon 19 of LAMB3, which has been mapped to chromosome 1q32. The patient was born with extensive blistering and demonstrated negative immunofluorescence staining for laminin 5, and transmission electron microscopy revealed tissue separation within lamina lucida of the dermal-epidermal junction, diagnostic of Herlitz junctional epidermolysis bullosa. The mother of the proband was found to be a heterozygous carrier for this mutation, whereas the father demonstrated the wild-type LAMB3 allele only. Nonpaternity was excluded by 13 microsatellite markers in six different chromosomes. Genotype analysis using 28 microsatellite markers spanning chromosome 1 revealed that the patient had maternal primary heterodisomy, as well as meroisodisomy within two regions of chromosome 1, one on 1p and the other one on 1q, the latter region containing the maternal LAMB3 mutation. These results suggest that Herlitz junctional epidermolysis bullosa in this patient developed as a result of reduction to homozygosity of the maternal LAMB3 mutation on chromosome 1q32. PMID:9579554

  19. Thermally driven microfluidic pumping via reversible shape memory polymers

    Science.gov (United States)

    Robertson, J. M.; Rodriguez, R. X.; Holmes, L. R., Jr.; Mather, P. T.; Wetzel, E. D.

    2016-08-01

    The need exists for autonomous microfluidic pumping systems that utilize environmental cues to transport fluid within a network of channels for such purposes as heat distribution, self-healing, or optical reconfiguration. Here, we report on reversible thermally driven microfluidic pumping enabled by two-way shape memory polymers. After developing a suitable shape memory polymer (SMP) through variation in the crosslink density, thin and flexible microfluidic devices were constructed by lamination of plastic films with channels defined by laser-cutting of double-sided adhesive film. SMP blisters integrated into the devices provide thermally driven pumping, while opposing elastic blisters are used to generate backpressure for reversible operation. Thermal cycling of the device was found to drive reversible fluid flow: upon heating to 60 °C, the SMP rapidly contracted to fill the surface channels with a transparent fluid, and upon cooling to 8 °C the flow reversed and the channel re-filled with black ink. Combined with a metallized backing layer, this device results in refection of incident light at high temperatures and absorption of light (at the portions covered with channels) at low temperatures. We discuss power-free, autonomous applications ranging from thermal regulation of structures to thermal indication via color change.

  20. Evaluation of miR-9 and miR-143 expression in urine specimens of sulfur mustard exposed patients

    Directory of Open Access Journals (Sweden)

    Khafaei Mostafa

    2015-12-01

    Full Text Available Sulfur mustard (SM or mustard gas is a chemical alkylating agent that causes blisters in the skin (blister gas, burns the eyes and causes lung injury. Some major cellular pathways are involved in the damage caused by mustard gas such as NF-κb signaling, TGF-β signaling, WNT pathway, inflammation, DNA repair and apoptosis. MicroRNAs are non-coding small RNAs (19–25 nucleotides that are involved in the regulation of gene expression and are found in two forms, extracellular and intracellular. Changes in the levels of extracellular microRNAs are directly associated with many diseases, it is thus common to study the level of extracellular microRNAs as a biomarker to determine the pathophysiologic status. In this study, 32 mustard gas injured patients and 32 healthy subjects participated. Comparative evaluation of miR-9 and miR-143 expression in urine samples was performed by Real Time PCR and Graph Pad software. The Mann Whitney t-test analysis of data showed that the expression level of miR-143 and miR-9 had a significant decrease in sulfur mustard individuals with the respective p-value of 0.0480 and 0.0272 compared to normal samples, with an imbalance of several above mentioned pathways. It seems that reducing the expression level of these genes has a very important role in the pathogenicity of mustard gas injured patients.

  1. Recognizing Presentations of Pemphigoid Gestationis: A Case Study

    Directory of Open Access Journals (Sweden)

    Sadie Henry

    2014-01-01

    Full Text Available Introduction. Pemphigoid gestationis (PG is an autoimmune blistering disease that occurs in approximately 1 in 50,000 pregnancies. Failing to recognize PG may lead to inadequate maternal treatment and possible neonatal complications. Case Report. At 18 weeks of gestation, a 36-year-old otherwise healthy Caucasian G4P1 presented with pruritic papules on her anterior thighs, initially treated with topical steroids. At 31 weeks of gestation, she was switched to oral steroids after her rash and pruritus worsened. The patient had an uncomplicated SVD of a healthy female infant at 37 weeks of gestation and was immediately tapered off steroid treatment, resulting in a severe postpartum flare of her disease. Discussion. This case was similar to reported cases of pruritic urticarial papules followed by blisters; however, this patient had palm, sole, and mucous membrane involvement, which is rare. Biopsy for direct immunofluorescence or ELISA is the preferred test for diagnosis. Previous case reports describe severe postdelivery flares that require higher steroid doses. Obstetrical providers need to be familiar with this disease although it is rare, as this condition can be easily confused with other dermatoses of pregnancy. Adequate treatment is imperative for the physical and psychological well-being of the mother and infant.

  2. The Experiment Production And Examination Of The U3Si2-AI Mini plates For Irradiation Test

    International Nuclear Information System (INIS)

    The fuel plates containing U3Si2-AI dispersion fuel having respective loading of 3.55; 4.20; and 4.80 g/cm3 were prepared by dispersing certain amount of U3Si2 powder in the AI powder as matrix. The weight ratio of U3Si2 and AI at different loading was chosen based on the 19.23 cm3 volume basis fuel core calculation. Each fuel mixture was pressed into a fuel core having dimension of 100.20 x 60.35 x 3.15 +- (0.05) mm, which was then cut into mini fuel core having dimension of 16 x 8 x 3.15 +- (0.05) mm. The mini plates were prepared by picture and frame technique using AIMg2 as cladding material. The mini plates have been tested for blister, homogeneity, white spots, surface defects and their cladding thickness, revealing that out of 74 mini plates, they are ten (10) mini plates that have to be rejected due to blisters and white spots, thus of 64 mini plates can be further fabricated as samples for irradiation test

  3. Detection of apoptosis in pemphigus vulgaris by TUNEL technique*

    Science.gov (United States)

    Cuevas-Gonzalez, Juan Carlos; Vega-Memíje, Maria Elisa; García-Vázquez, Francisco Javier; Aguilar-Urbano, Marco António

    2016-01-01

    Background Pemphigus is part of a group of blistering diseases that affect the skin and mucous membranes. Based on its autoimmune origin, autoantibodies develop in pemphigus that are directed toward cell surface components of keratinocytes. However, some data cannot be explained, such as the lack of a relationship between autoantibody levels and the severity of clinical manifestations, treatment resistance, the presence of inflammatory infiltrates and the potential occurrence of apoptosis as determinants of vesicle formation. Objective To examine the presence of apoptosis in pemphigus vulgaris by TUNEL technique. Methods In this cross-sectional study, we selected 15 paraffin-embedded tissues from subjects who were diagnosed with pemphigus vulgaris by hematoxylin and eosin staining. The samples were subjected to TUNEL assay and examined under an Olympus BX61 fluorescence microscope. Positivity was categorized dichotomously, and the statistical analysis was performed using the X2 test. Results Positivity was observed in basal layer cells in 14 (93.3%) cases. In 13 (86.7%) of the positive cases, we noted espinosum and granular layers that formed the blister roof, and in 12 cases (80%), positive acantholytic cells were observed. Conclusions TUNEL positivity was observed in pemphigus vulgaris, implicating apoptosis in the pathophysiology of this condition, which can help guide the development of apoptotic blockers as therapeutics. PMID:27438195

  4. Monitorization of drug content in furosemide and lorazepam tablets stored in multidose pill boxes

    Directory of Open Access Journals (Sweden)

    Jessica Martins

    2010-01-01

    Full Text Available Background : Therapeutic nonadherence is a major health problem, particularly when therapeutic regimens are complex and long-lasting. Therefore, tools such as multidose pill boxes have been designed to provide the means for higher therapeutic compliance. However, no studies are available reporting on their capacity to keep the drug content of the stored tablets unaltered. Objective : This work aimed at monitoring the drug content of tablets stored in multidose boxes for a period of two weeks. Materials and Methods : Furosemide and lorazepam were selected as model drugs, given their frequent chronic use, which is coherent with the profile of medicines susceptible of storage in the referred boxes. Variations of the tablets drug content were assessed as a function of temperature (25°C and 40°C and the presence of blister. Results and Discussion : The obtained results allowed concluding that concerning temperature, only lorazepam tablets registered drug content alterations and only when stored at 40°C. On the other side, it was concluded that the absence of blister does not compromise the drug content of the studied tablets. Conclusion : In the specific conditions of this study, the storage of these medicines in multidose boxes is considered reliable and adequate.

  5. [Forensic-medical diagnostics of an electrical mark resulting from the injury inflicted by technical electricity in the aqueous environment].

    Science.gov (United States)

    Pigolkin, Iu I; Skovorodnikov, S V; Dubrovin, I A

    2014-01-01

    The objective of the present study was to develop the criteria for forensic-medical diagnostics of an electrical injury inflicted in the aqueous environment based on the characteristics of the electrical mark. The specific morphological features of the electrical injuries inflicted in the aqueous environment that were discovered in the materials available for the forensic medical expertise were analysed taking into consideration the results of the relevant research reported in the forensic medical literature. It was shown that an electrical injury inflicted in the aqueous environment results in the formation of an unusual mark in the form of blisters containing no watery liquid associated with electrogenic oedema in the surrounding tissues. Macroscopic and microscopic studies of the electrical mark failed to reveal the signs of grade III and IV grade thermal burning or thermally affected hair. It is concluded that the consistent characteristics of the electrical mark resulting from the injury inflicted by technical electricity in the aqueous environment include cell lengthening, blister formation inside the corneal layer, and the separation of epidermis from the skin proper.

  6. The temperature and dose dependency of helium reemission ratio from helium-ion-implanted aluminium samples

    International Nuclear Information System (INIS)

    It has been observed that blisters and flakes appear on a metal surface implanted He ions above a specific ion dose called the critical dose. At the appearance of the blister and flake, He gas responsible for them is released. There are many reports on the gas reemission with these deformations but there are few reports about it below the critical dose. In this report, temperature and dose dependencies of He reemission from He-ion-implanted Al samples have been studied. After He ions of 20 keV are implanted in Al samples, the samples are heated in vacuum at 400, 450, 475, and 500 deg C with holding time of 0.5, 1, and 2 hours. The numbers of He atoms remaining in the sample are measured by a He atom measurement system. The reemission ratios of the samples prepared at the given temperature and holding time have been obtained from comparison of the numbers of He ions implanted in the samples with the numbers of He atoms measured by the He atom measurement system. The reemission ratio rises with increasing temperature and holding time, and achieved at ∼ 90 % with 500 deg C and 2 hours holding time. On dose dependency of the reemission ratio, it is constant up to 7 x 1014 He atoms and it increases rapidly above it. (author)

  7. Analysis of LOCA Scenarios in the NIST Research Reactor Before and After Fuel Conversion

    Energy Technology Data Exchange (ETDEWEB)

    Baek, J. S. [Brookhaven National Lab. (BNL), Upton, NY (United States); Cheng, L. Y. [Brookhaven National Lab. (BNL), Upton, NY (United States); Diamond, D. [Brookhaven National Lab. (BNL), Upton, NY (United States)

    2015-08-30

    An analysis has been done of hypothetical loss-of-coolant-accidents (LOCAs) in the research reactor (NBSR) at the National Institute of Standards and Technology (NIST). The purpose of the analysis is to determine if the peak clad temperature remains below the Safety Limit, which is the blister temperature for the fuel. The configuration of the NBSR considered in the analysis is that projected for the future when changes will be made so that shutdown pumps do not operate when a LOCA signal is detected. The analysis was done for the present core with high-enriched uranium (HEU) fuel and with the proposed low-enriched uranium (LEU) fuel that would be used when the NBSR is converted from one to the other. The analysis consists of two parts. The first examines how the water would drain from the primary system following a break and the possibility for the loss of coolant from within the fuel element flow channels. This work is performed using the TRACE system thermal-hydraulic code. The second looks at the fuel clad temperature as a function of time given that the water may have drained from many of the flow channels and the water in the vessel is in a quasi-equilibrium state. The temperature behavior is investigated using the three-dimensional heat conduction code HEATING7.3. The results in all scenarios considered for both HEU and LEU fuel show that the peak clad temperature remains below the blister temperature.

  8. Accelerated low water corrosion of carbon steel in the presence of a biofilm harbouring sulphate-reducing and sulphur-oxidising bacteria recovered from a marine sediment

    Energy Technology Data Exchange (ETDEWEB)

    Beech, Iwona B. [Applied Microbiology and Electrochemistry Group, University of Portsmouth, St. Michael' s Building, White Swan Road, Portsmouth PO1 2DT (United Kingdom)], E-mail: iwona.beech@port.ac.uk; Campbell, Sheelagh A. [Applied Microbiology and Electrochemistry Group, University of Portsmouth, St. Michael' s Building, White Swan Road, Portsmouth PO1 2DT (United Kingdom)

    2008-12-01

    Investigations were undertaken to elucidate causes of accelerated low water corrosion (ALWC) of steel piling in a harbour in Southern England. Visual inspection revealed features characteristic of ALWC such as the presence of poorly adherent, thick corrosion products of varying morphology, often seen as large blisters randomly located on sections of the structure at the low water mark. Upon the removal of blisters, a bright surface covered with shallow pits was exposed. Representative samples of the corrosion products were collected from the structure and water and sediment specimens were retrieved from selected areas in the harbour for microbiological, chemical and microscopy testing. In the laboratory, field samples were enriched to detect and enumerate communities of sulphur-oxidising bacteria (SOB) and sulphate-reducing bacteria (SRB). Biofilms, comprising SRB and SOB populations isolated from a sediment sample were grown under static conditions on surfaces of electrodes manufactured from steel piling material. Linear polarisation resistance (LPR) measurements revealed that the corrosion rate of steel with biofilms (0.518 mm y{sup -1}) was higher than that recorded in sterile seawater alone (0.054 mm y{sup -1}) and in sterile seawater to which nutrient was added (0.218 mm y{sup -1}). Scanning electron microscopy (SEM) imaging demonstrated enhanced pitting under biofilms. The results of our investigation revealed for the first time that the attack on steel piling in the presence of sediment SRB and SOB populations was characteristic of ALWC.

  9. Influence of Environment on Ageing Behaviour of the Polyurethane Film

    Directory of Open Access Journals (Sweden)

    Yanzhi WANG

    2016-05-01

    Full Text Available In this work, UV-Vis spectra, FT-IR spectra, colour difference, yellowness index,and SEM micrographs were used to study the accelerated ageing behaviour of polyurethane films that exposed to UV radiation, O3 atmosphere, and UV/O3 integrated environment. During 200 hours of exposure in three different environment, the UV absorbance, the colour difference, the yellowness, and the absorption of –NH/–OH and –C=O bands in FT-IR spectra of the films increase gradually with increasing exposure time, respectively, and the films exposed to the three environments have different colour difference, yellowness index, UV-Vis spectra, and FT-IR spectra. The films are vulnerable to degradation and yellowing in the following environment order: O3 < UV < UV/O3. After exposure to UV radiation or O3 atmosphere, some degradation products and blisters are formed on the film surface. After exposure to UV/O3 integrated environment, there are strip blisters and micro-cracks on the film surface, and exists an obvious synergism between UV radiation and O3 atmosphere in accelerating the ageing of the polyurethane films.DOI: http://dx.doi.org/10.5755/j01.ms.22.2.12935

  10. Correlation between surface damage and micro-defects in Si covered with insulating layer by implantation of He and H ions

    Energy Technology Data Exchange (ETDEWEB)

    Liu, C.L., E-mail: liuchanglong@tju.edu.c [School of Science, Tianjin University, Tianjin 300072 (China); Tianjin Key Laboratory of Low Dimension Materials Physics and Preparing Technology, Institute of Advanced Materials Physics Faculty of Science, Tianjin 300072 (China); Li, M.K.; Wang, Z.; Gao, Y.J.; Liao, J.Q.; Zhang, D.C.; Zhang, X.L.; Shen, Y.Y. [School of Science, Tianjin University, Tianjin 300072 (China)

    2011-03-01

    Cz n-type Si (100) wafers covered with a 220 nm SiO{sub 2} layer or a 170 nm Si{sub 3}N{sub 4} layer were singly implanted with 160 keV He ions at a dose of 5 x 10{sup 16}/cm{sup 2} or successively implanted with 160 keV He ions at a dose of 5 x 10{sup 16}/cm{sup 2} and 110 keV H ions at a dose of 1 x 10{sup 16}/cm{sup 2}. Surface morphologies together with defect microstructures have been studied by means of several techniques, including optical microscopy, atomic force microscopy, and cross-sectional transmission electron microscopy (XTEM). Only surface blistering has been observed for He and H sequentially implanted SiO{sub 2}/Si samples after annealing in temperature range up to 1000 {sup o}C. However, as for the He and H implanted Si{sub 3}N{sub 4}/Si samples, surface features including blistering and the localized exfoliation of both the top Si{sub 3}N{sub 4} layer and the implanted Si layer have been well demonstrated during subsequent annealing. XTEM observations reveal quite different defect morphologies in two kinds of materials under the same implantation and annealing conditions. The possible mechanisms of surface damage in two kinds of materials have been discussed and presented based on the XTEM results.

  11. Studies of vesicle distribution patterns in Hawaiian lavas

    Science.gov (United States)

    Walker, George P. L.

    1987-01-01

    Basaltic lava flows are generally vesicular, and the broader facts relating to vesicle distribution have long been established; few studies have yet been made with a view to determining how and when vesicles form in the cooling history of the lava, explaining vesicle shape and size distribution, and gaining enough understanding to employ vesicles as a geological tool. Various avenues of approach exist by which one may seek to gain a better understanding of these ubiquitous structures and make a start towards developing a general theory, and three such avenues have recently been explored. One avenue involves the study of pipe vesicles; these are a well known feature of lava flows and are narrow pipes which occur near the base of many pahoehoe flow units. Another avenue of approach is that presented by the distinctive spongy pahoehoe facies of lava that is common in distal locations on Hawaiian volcanoes. A third avenue of approach is that of the study of gas blisters in lava. Gas blisters are voids, which can be as much as tens of meters wide, where the lava split along a vesicle-rich layer and the roof up-arched by gas pressure. These three avenues are briefly discussed.

  12. Evaluation of miR-9 and miR-143 expression in urine specimens of sulfur mustard exposed patients.

    Science.gov (United States)

    Khafaei, Mostafa; Samie, Shahram; Mowla, Seyed Javad; Alvanegh, Akbar Ghorbani; Mirzaei, Behnaz; Chavoshei, Somaye; Dorraj, Ghamar Soltan; Esmailnejad, Mostafa; Tavallaie, Mahmood; Nourani, Mohammadreza

    2015-12-01

    Sulfur mustard (SM) or mustard gas is a chemical alkylating agent that causes blisters in the skin (blister gas), burns the eyes and causes lung injury. Some major cellular pathways are involved in the damage caused by mustard gas such as NF-κb signaling, TGF-β signaling, WNT pathway, inflammation, DNA repair and apoptosis. MicroRNAs are non-coding small RNAs (19-25 nucleotides) that are involved in the regulation of gene expression and are found in two forms, extracellular and intracellular. Changes in the levels of extracellular microRNAs are directly associated with many diseases, it is thus common to study the level of extracellular microRNAs as a biomarker to determine the pathophysiologic status. In this study, 32 mustard gas injured patients and 32healthy subjects participated. Comparative evaluation of miR-9 and miR-143 expression in urine samples was performed by Real Time PCR and Graph Pad software. The Mann Whitney t-test analysis of data showed that the expression level of miR-143 and miR-9 had a significant decrease in sulfur mustard individuals with the respective p-value of 0.0480 and 0.0272 compared to normal samples, with an imbalance of several above mentioned pathways. It seems that reducing the expression level of these genes has a very important role in the pathogenicity of mustard gas injured patients. PMID:27486378

  13. Effect of plastic deformation on deuterium retention and release in tungsten

    Energy Technology Data Exchange (ETDEWEB)

    Terentyev, D., E-mail: dterenty@sckcen.be; Lambrinou, K.; Minov, B. [SCK-CEN, Nuclear Materials Science Institute, Boeretang 200, 2400 Mol (Belgium); De Temmerman, G. [ITER Organization, Route de Vinon-sur-Verdon - CS 90 046 - 13067 St. Paul Lez Durance Cedex (France); Morgan, T. W. [FOM Institute DIFFER, Edisonbaan 14, 3439 MN, Nieuwegein (Netherlands); Zayachuk, Y.; Bystrov, K. [Department of Materials, University of Oxford, Parks Road, Oxford OX1 3PH (United Kingdom); Dubinko, A. [SCK-CEN, Nuclear Materials Science Institute, Boeretang 200, 2400 Mol (Belgium); Department of Applied Physics, Ghent University, St. Pietersnieuwstraat 41, 9000 Ghent (Belgium); Van Oost, G. [Department of Applied Physics, Ghent University, St. Pietersnieuwstraat 41, 9000 Ghent (Belgium)

    2015-02-28

    The effect of severe plastic deformation on the deuterium retention in tungsten exposed to high-flux low-energy plasma (flux ∼ 10{sup 24 }D/m{sup 2}/s, energy ∼ 50 eV, and fluence up to 3 × 10{sup 26 }D/m{sup 2}) at the plasma generator Pilot-PSI was studied by thermal desorption spectroscopy and scanning electron microscopy. The desorption spectra in both reference and plastically deformed samples were deconvolved into three contributions attributed to the detrapping from dislocations, deuterium-vacancy clusters, and pores, respectively. The plastically induced deformation, resulting in high dislocation density, does not change the positions of the three peaks, but alters their amplitudes as compared to the reference material. The appearance of blisters detected by scanning electron microscopy and the desorption peak attributed to the release from pores (i.e., deuterium bubbles) were suppressed in the plastically deformed samples but only up to a certain fluence. Beyond 5 × 10{sup 25 }D/m{sup 2}, the release from the bubbles in the deformed material is essentially higher than in the reference material. Based on the presented results, we suggest that a dense dislocation network increases the incubation dose needed for the appearance of blisters, associated with deuterium bubbles, by offering numerous nucleation sites for deuterium clusters eventually transforming into deuterium-vacancy clusters by punching out jogs on dislocation lines.

  14. Occurrence, Distribution and Biological variability of Zucchini Yellow Mosaic Virus in cucurbits of Khuzestan province, South west of Iran

    Directory of Open Access Journals (Sweden)

    Somayeh Safara

    2011-11-01

    Full Text Available ZYMV is one of the most important plant viruses that cause economical damage in cucurbits. The symptoms of ZYMV in different cucurbits include stunting, yellowing, mottling, severe mosaic, leaf and fruit deformation, blistering and shoe string. Investigation on occurrence of this virus, in Khuzestan province was carried out in November 2009, April and May 2010 by collecting cucurbits samples from different cucurbits fields. After DAS-ELISA test, ZYMV was maintained in squash. Then total RNA were extracted and were tested by RT-PCR. Using RT-PCR, fragments belonging to N-terminal of coat protein and C-terminal of nuclear inclusion bodies were replicated. PCR product for investigation of replication was loaded in 1% agarose gel. From seven regions in Khuzestan, 175 leaf samples showing different symptoms (yellowing, mosaic, deformation and blistering were collected. Seventy one samples out of total samples (175 samples showed ZYMV infection. Occurrence of Zucchini Yellow Mosaic Virus in Khuzestan province was confirmed, using serological and RT-PCR tests. Infection of ZYMV in Khuzestan province (40.5% is higher than the average of Iran’s infection (38%. This article is first report of occurrence ZYMV in different regions of Khuzestan province except Dezful.

  15. Association among active seafloor deformation, mound formation, and gas hydrate growth and accumulation within the seafloor of the Santa Monica Basin, offshore California

    Science.gov (United States)

    Paull, C.K.; Normark, W.R.; Ussler, W.; Caress, D.W.; Keaten, R.

    2008-01-01

    Seafloor blister-like mounds, methane migration and gas hydrate formation were investigated through detailed seafloor surveys in Santa Monica Basin, offshore of Los Angeles, California. Two distinct deep-water (??? 800??m water depth) topographic mounds were surveyed using an autonomous underwater vehicle (carrying a multibeam sonar and a chirp sub-bottom profiler) and one of these was explored with the remotely operated vehicle Tiburon. The mounds are > 10??m high and > 100??m wide dome-shaped bathymetric features. These mounds protrude from crests of broad anticlines (~ 20??m high and 1 to 3??km long) formed within latest Quaternary-aged seafloor sediment associated with compression between lateral offsets in regional faults. No allochthonous sediments were observed on the mounds, except slumped material off the steep slopes of the mounds. Continuous streams of methane gas bubbles emanate from the crest of the northeastern mound, and extensive methane-derived authigenic carbonate pavements and chemosynthetic communities mantle the mound surface. The large local vertical displacements needed to produce these mounds suggests a corresponding net mass accumulation has occurred within the immediate subsurface. Formation and accumulation of pure gas hydrate lenses in the subsurface is proposed as a mechanism to blister the seafloor and form these mounds. ?? 2008 Elsevier B.V. All rights reserved.

  16. Molecular mapping of the Pinus monticola Cr2 gene using AFLP and SCAR markers

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    A.K.M. Ekramoddoullah

    2013-12-01

    Full Text Available White pine blister rust (WPBR, caused by Cronartium ribicola, is a devastating disease in five-needle pines. Genetic resistance is an important component of integrated strategies to control WPBR. The major resistance gene Cr2, discovered by Kinloch etal.(1999, is also effective against British Columbia (BC isolates of WPBR (Hunt et al. 2004. Pyramiding Cr2 gene with other resistancegenes is being pursued as a strategy in BC white pine breeding. To facilitate this strategy, we have recently identified a few RAPD markerslinked to Cr2 at one side (Liu et al. 2006. The objective of the present study was to identify amplified fragment length polymorphism(AFLP markers linked to both sides of Cr2 for its more precise apping. Use of the AFLP technique combined with bulked segregant analysis (BSA and haploid segregation analysis allowed the identification of five AFLP markers. Of these five AFLP markers in the Cr2 linkage, markers EacccMccgat-365, EactgMcccac- 290, and EacagEacag-750 werelinked in coupling and EacagMcccag-160r and EacccMccgat-180r in repulsion. Following cloning and sequencing of the AFLP andRAPD markers, specific PCR primers were designed and used in the amplification of sequence characterized amplified region(SCAR markers at both sides of Cr2. EacccMccgat- 365 and RAPD marker U570-843 reported previously were converted into SCARmarkers. These two SCARs segregated in a 1:1 (presence:absence ratio and the scoring cosegregated with their respective AFLP orRAPD marker. The SCAR marker EacccMccgat- 365-scar was positioned at 3.1 Kosambi cM from one side of Cr2 and U570-843-scarlocalized at 1.4 Kosambi cM from other side. Both SCAR markers can be useful in breeding programs with marker-assisted selection procedureto screen for resistance. This study represents the first report of the development of PCR-based sequence-specific markers linkedto blister rust resistance in five-needle pines. These findings may

  17. Growth, straightness and survival at age 32 in a Pinus strobus x P. wallichiana F1 hybrid population (Experiment 2

    Directory of Open Access Journals (Sweden)

    Ioan Blada

    2013-07-01

    Full Text Available By using an incomplete factorial mating design between twenty Pinus strobus L. female and seven P. wallichiana Jack. male trees, a number of 34 full-sib families were obtained. The objective of this experiment was to combine the fast growing of the former species with moderately to high resistance to blister rust (Cronartium ribicola Fich. in Rabenh. of the latter one. The hybrid families were artificially inoculated at age two, and field planted at age four. The plantation took place within an old black current (Ribes nigrum L. heavy infected by blister rust. In order to provoke new infections, this time naturally, the pine rows were planted in between the black currant ones. Diameters at breast height, tree height, tree growth rate volume, stem straightness and tree survivals were the traits measured at age 32 from seed. The first trial thinning was simultaneously applied with the present measurements. The average tree survival was 74.8% in hybrids, 8.3% in Pinus strobus and 27.8% in P. wallichiana. Highly significant (p < 0.01 differences were found between hybrid families for all traits except stem straightness. Genetic coefficient of variation at family level was 13.7% for tree volume growth rate and 15.9% for tree survival, but only 2.1% for tree straightness. Broad-sense family heritability estimates were 0.530 for diameter at breast height, 0.596 for stem height, and 0.564 for stem volume growth rate, 0.166 for stem straightness, and 0.539 for tree survivals. Similarly, the individual tree narrow-sense heritability estimates were 0.138 for diameter at breast height, 0.209 for stem height, 0.149 for stem volume growth rate, and 0.022 for stem straightness. If the best 5, 10 and 15 of 34 families were selected, a genetic gain of 17.7%, 13.4% and 10.2%, respectively, may be achieved in tree survival or blister rust resistance. Similarly, if the best 5%, 10% and 15% individuals within the best hybrid families were selected, a genetic gain of

  18. Growth, straightness and survival at age 32 in a Pinus strobus x P. wallichiana F1 hybrid population (Experiment 2

    Directory of Open Access Journals (Sweden)

    Ioan Blada

    2013-05-01

    Full Text Available By using an incomplete factorial mating design between twentyPinus strobus L. female and seven P. wallichiana Jack. male trees, a number of 34 full-sib families were obtained. The objective of this experiment was to combine the fast growing of the former species with moderately to high resistance to blister rust (Cronartium ribicola Fich. in Rabenh. of the latter one. The hybrid families were artificially inoculated at age two, and field planted at age four. The plantation took place within an old black current (Ribes nigrum L. heavy infected by blister rust. In order to provoke new infections, this time naturally, the pine rows were planted in between theblack currant ones. Diameters at breast height, tree height, tree growth rate volume, stem straightness and tree survivals were the traits measured at age 32 from seed. The first trial thinning was simultaneously applied with the present measurements. The average tree survival was 74.8% in hybrids, 8.3% in Pinus strobus and 27.8% in P. wallichiana. Highly significant (p < 0.01 differences were found between hybrid families for all traits except stem straightness. Genetic coefficient of variation at family level was 13.7% fortree volume growth rate and 15.9% for tree survival, but only 2.1% for tree straightness. Broad-sense family heritability estimates were 0.530 for diameter at breast height, 0.596 for stem height, and 0.564 for stem volume growth rate, 0.166 for stem straightness, and 0.539 for tree survivals. Similarly, the individual tree narrow-sense heritability estimates were 0.138 for diameter at breast height, 0.209 for stem height, 0.149 for stem volume growth rate, and 0.022 for stem straightness. If the best 5, 10 and 15 of 34 families wereselected, a genetic gain of 17.7%, 13.4% and 10.2%, respectively, may be achieved in tree survival or blister rust resistance. Similarly, if the best 5%, 10% and 15% individuals within the best hybrid families were selected, a genetic gain of 4

  19. He reemission implanted in metals

    Energy Technology Data Exchange (ETDEWEB)

    Tanabe, T., E-mail: tanabe@nucl.kyushu-u.ac.jp

    2014-10-15

    Highlights: • Observation of He reemission of various metals under He{sup +} implantation at wide temperature range. • Materials examined are aluminum (Al), Nickel (Ni) and molybdenum (Mo). • He reemission is quite temperature dependent and different with materials. • Three metals show similar dependence on temperature normalized with respective melting point. • He reemission is successfully correlated with He behavior in metals. - Abstract: Helium (He) reemission of Al, Ni and Mo under energetic He implantation (10–30 keV) in wide temperature range is studied to understand behavior of implanted He in correlation with structure changes. The reemission behavior is categorized into 4 different temperature ranges with the normalized temperature (T{sub m}) to the melting point of each metal. At elevated temperatures (well above ∼0.6 T{sub m}), interstitial He atoms and/or He-vacancy (ies) clusters can migrate remaining no structure change and showing smooth reemission without any burst. Between ∼0.25 and 0.6 T{sub m}, He reemission always accompanies significant structure modification. For ∼04–0.6 T{sub m}, implanted He coalesce to make bubbles and the bubbles can move to the surface. Bubble migration accompanies materials flow to the surface resulting in fuzz surface or columnar structure, depending on implantation flux. Slower bubble motion at ∼0.25–0.4 prohibits the material migration. Instead the bubbles coalesce to grow large and multi-layered blistering appears as periodic reemission behavior. Below ∼0.25 T{sub m}, He migration is too slow for bubbles to grow large, but bubble density increases up to a certain fluence, where neighboring bubbles start to coalesce. Accordingly, He release is mostly caused by mechanical failure or blister rapture. With increasing fluence, all defects (bubbles and dislocation loops) tangle or inter connected with neighboring defects and accordingly He migration to the surface along the tangled or connected

  20. Effect of Packaging on Stability of Simvastatin Tablets%泡罩包装对辛伐他汀片剂稳定性的影响

    Institute of Scientific and Technical Information of China (English)

    郭冬薇; 沈园园; 陈伟峦; 郭圣荣

    2011-01-01

    Three types of packages [polyvinyl chloride (PVC)-aluminum (Al), polyvinyl dichloride (PVDC)-Al and Al-Al blisters] were used to investigate the effect of packaging on stability of moisture sensitive simvastatin tablets. The content changes of simvastatin and its related substances and the container moisture permeability of blisters were studied to evaluate the effects. Three related substances of simvastatin were identified to be simvastatin acid, lovastatin and lovastatin acid by HPLC and LC-MS. The increase of related substances of simvastatin was mainly attributed to the increase of simvastatin acid content, implying that the degradation of simvastatin was primarily caused by hydrolysis. It was proposed that the stability of simvastatin tablets was highly related to the moisture permeability of package. Packages with excellent humidity resistance, such as Al-Al blisters, would be beneficial to improve the stability of simvastatin tablets.%考察了3种包装泡罩[聚氯乙烯-铝(PVC-A1)、聚偏二氯乙烯/聚氯乙烯涂复片-铝(PVDC-Al)和双铝(Al-Al)包装泡罩]对湿敏性药物辛伐他汀片剂稳定性的影响.通过检测辛伐他汀片的药物含量与有关物质含量随时间的变化,评价包装材料的影响.采用HPLC法和LC-MS法确定了辛伐他汀的3个有关物质,分别为辛伐他汀酸、洛伐他汀以及洛伐他汀酸.其中,辛伐他汀酸为辛伐他汀的水解产物.有关物质随时间的变化主要是由辛伐他汀酸的增加引起的,表明辛伐他汀的降解主要是水解.抗湿性最强的A1-Al泡罩中辛伐他汀片剂的稳定性优于PVDC-Al和PVC-A1泡罩,证实了辛伐他汀片剂的稳定性与包装材料的抗湿性高度相关.

  1. Adherence of community caretakers of children to pre-packaged antimalarial medicines (HOMAPAK® among internally displaced people in Gulu district, Uganda

    Directory of Open Access Journals (Sweden)

    Opwonya John

    2006-05-01

    Full Text Available Abstract Background In 2002, home-based management of fever (HBMF was introduced in Uganda, to improve access to prompt, effective antimalarial treatment of all fevers in children under 5 years. Implementation is through community drug distributors (CDDs who distribute pre-packaged chloroquine plus sulfadoxine-pyrimethamine (HOMAPAK® free of charge to caretakers of febrile children. Adherence of caretakers to this regimen has not been studied. Methods A questionnaire-based survey combined with inspection of blister packaging was conducted to investigate caretakers' adherence to HOMAPAK®. The population surveyed consisted of internally displaced people (IDPs from eight camps. Results A total of 241 caretakers were interviewed. 95.0% (CI: 93.3% – 98.4% of their children had received the correct dose for their age and 52.3% of caretakers had retained the blister pack. Assuming correct self-reporting, the overall adherence was 96.3% (CI: 93.9% – 98.7%. The nine caretakers who had not adhered had done so because the child had improved, had vomited, did not like the taste of the tablets, or because they forgot to administer the treatment. For 85.5% of cases treatment had been sought within 24 hours. Blister packaging was considered useful by virtually all respondents, mainly because it kept the drugs clean and dry. Information provided on, and inside, the package was of limited use, because most respondents were illiterate. However, CDDs had often told caretakers how to administer the treatment. For 39.4% of respondents consultation with the CDD was their reported first action when their child has fever and 52.7% stated that they consult her/him if the child does not get better. Conclusion In IDP camps, the HBMF strategy forms an important component of medical care for young children. In case of febrile illness, most caretakers obtain prompt and adequate antimalarial treatment, and adhere to it. A large proportion of malaria episodes are thus

  2. A case of epidermolysis bullosa acquisita in childhood%儿童获得性大疱性表皮松解症一例

    Institute of Scientific and Technical Information of China (English)

    姜祎群; 曾学思; 陈声利; 冯素英; 陈浩

    2011-01-01

    报道1例儿童获得性大疱性表皮松解症.患者女,12岁,因全身反复水疱、大疱和糜烂面3个月入院.患者弱智,父母非近亲结婚,无家族史.入院时表现全身大片糜烂面,包括手足、膝关节周围和股臀部,同时躯干、四肢正常或水肿性红斑基础上紧张性水疱、大疱.治疗过程中突然发生躯干、四肢大量紧张性水疱.组织病理提示,表皮下水疱,免疫荧光结果见表皮基底膜带IgG和C3线状沉积,盐裂后沉积限于真皮侧.ELISA检测BP180和BP230阴性.采用大剂量糖皮质激素联合四环素口服取得满意疗效.%A case of epidermolysis bullosa acquisita (EBA) in childhood is reported. A 12-year-old girl was hospitalized for a 3-month history of recurrent blisters, bullae and erosions on the trunk and limbs. The girl had mental retardation but no family history of similar disorders. The marriage between her parents was not consanguineous. Physical examination on admission revealed large erosions with moderate oozing on the hands and feet, around the knees and on the buttock and thighs. There were scattered tense blisters and bullae arising in normal skin or edematous erythema on the trunk and limbs. During the treatment course the patient suddenly developed a number of tense blisters over the whole integument on the trunk and limbs. Skin biopsy showed subepidermal bullae with moderate perivascular infiltration of neutrophils and eosinophils. Direct immunofluorescence (DIF) revealed linear IgG and C3 deposition along the basal membrane zone, which was on the dermal side of salt-split skin on indirect immunofluorescence (IIF). ELISA detected no serum antiBP180 or -BP230 antibodies in the patient. A diagnosis of EBA was made. The patient was successfully controlled by intravenous steroids combined with oral tetracycline.

  3. Fluconazole-induced Stevens-Jonson syndrome

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    Umashanker P. Keshri

    2014-06-01

    Full Text Available A 25-year-old girl self-administered tab fluconazole for vulvovaginal candidiasis and developed fever, myalgia and erythematous papular eruptions with itching at both upper and lower lip, mucous membrane of the mouth, vaginal region, which subsequently changed into blister and multiple ulcers. She also had difficulty in swallowing due to painful erosions of the mouth and oropharynx and severe burning pain at vulval and vaginal region. There was bilateral conjunctival hemorrhage. Investigation report revealed leukocytosis and elevated C-reactive protein, which was suggesting the diagnosis of Stevens-Johnson syndrome. She was treated mainly by corticosteroids, antihistamines and antimicrobials and improved. Time taken for resolution of the lesion was 14 days. Rechallenge with the offending drug was not done in the interest of the patient and due to ethical constraints. [Int J Basic Clin Pharmacol 2014; 3(3.000: 566-568

  4. Recent advancement on autoantigens, autoantibodies and inflammatory cells in subepidermal autoimmune bullous diseases

    Institute of Scientific and Technical Information of China (English)

    Jianguo Huang; Qiying Gong; Guiming Li

    2007-01-01

    Subepidermal autoimmune bullous diseases (SABD) are some autoimmune skin diseases that can present in a variety of forms and can be a challenging disease to treat. An overview of the different forms of SABD are discussed including bullous pemphigoid (BP), epidermolysis bullosa acquisita (EBA), cicatricial pemphigoid (CP), bullous systemic lupus erythematosus (BSLE), and Anti-p200 pemphigoid. Emphasis on recent advancement is presented. In recent years, improved Knowledge of the mechanisms of intercellular and cell-matrix adhesion has led to better understanding of the blistering process in some SABD. Defects of such structures cause the subepidermal bullous diseases and have also led to the discovery of new diseases (e.g. anti-p200-pemphigoid). Recent studies have outlined the important role of autoantibodies, mast cell lymphocytes and their cytokines in pathogenesis of SABD.

  5. Bullous pemphigoid and neurodegenerative diseases: a study in a setting of a Central European university dermatology department.

    Science.gov (United States)

    Pietkiewicz, Paweł; Gornowicz-Porowska, Justyna; Bowszyc-Dmochowska, Monika; Bartkiewicz, Paweł; Dmochowski, Marian

    2016-08-01

    Bullous pemphigoid (BP) is an autoimmune blistering dermatosis of the elderly mediated by IgG and IgE antibodies to skin hemidesmosomal proteins, BP180 and/or BP230, that occur physiologically also in neuronal tissue. It was reported that BP is associated with neurodegenerative diseases (ND). We performed a retrospective study in a setting of a Central European university dermatology department on prevalence of ND in 94 BP patients. 26 out of 94 BP patients had at least one ND. ND included: Parkinson's disease, dementia, stroke, hear loss, tinnitus, blindness, vertigo, neurosyphilis, systemic sclerosis, and epilepsy. Since population aging is conceivably responsible for the rising number of BP cases as a result of immunosenescence-related phenomena, the plausible BP-specific immunopathogenetic relationship between BP and ND deserves to be further experimentally explored. PMID:26420424

  6. Chronic Foot Pain due to Pachyonychia Congenita in a Pediatric Patient: A Successful Management Strategy.

    Science.gov (United States)

    Tariq, Sarah; Schmitz, Michael L; Kanjia, Megha Karkera

    2016-05-15

    We report the case of an 11-year-old girl who presented to our multidisciplinary pain center with the chief complaint of chronic bilateral foot pain because of a rare congenital keratin disorder. This patient had been diagnosed with pachyonychia congenita, an extremely rare genetic disorder primarily affecting the skin and nails. The child had bilateral foot pain for years because of the characteristic blisters and calluses on the soles of her feet. Chronic pain was negatively impacting her quality of life; she was severely limited in her activities of daily living secondary to pain. Furthermore, she reported absenteeism from school, lack of social activities, and frequent nighttime awakenings. We discuss the successful management of her chronic foot pain using a multimodal, multidisciplinary approach. PMID:27182712

  7. Analysis of the pressure tube failure at Pickering NGS A unit 2

    International Nuclear Information System (INIS)

    The failure of a Zircaloy-2 pressure tube in Pickering Unit 2 in August 1983 has been found to have been caused by an accelerated pickup rate of deuterium, contact between the pressure tube and its surrounding calandria tube, and rapid growth of zirconium hydride blisters. The pressure tubes in all later CANDU reactors are made from zirconium- niobium alloy. Examination of several Zircaloy-2 and zirconium niobium pressure tubes from different reactors has clearly shown that the deuterium pickup rate of the two materials is significantly different. The zirconium niobium pressure tubes have absorbed very little deuterium and they do not appear to be susceptible to the type of failure experienced at Pickering Unit 2

  8. TERAPI KORTIKOSTEROID PADA PEMPHIGOID MEMBRAN MUKOSA MULUT DAN EFEK SAMPINGNYA (LAPORAN KASUS

    Directory of Open Access Journals (Sweden)

    Andrian Nova Fitri

    2015-07-01

    Full Text Available Mucous membrane pemphigoid (MMP, also known as cicatrical pemphigoid, is a chronic, autoimmune, subepithelial blistering disease primarily affecting people over 50 years old. MMP may affect any mucosal surface, which is particularly oral mucosa, conjuctiva, esophagus, or larynx is involved. MMP rarely occurs in children and adolescents, but several cases of MMP affecting 2 to 18 years old patients old male patient who had been diagnosed with oral mucous membrane pemphigoid by anamnesis, clinical feature, histopathology. The patient was treated with systemic corticosteroid, topical corticosteroid, oral rinse and multivitamins. Until recently corticosteroid remains as the most effective and the drug of choice to treat pemphigoid lesions. Clinicians need to pay attention in using this drug because of its potential side effect.

  9. Data on HLA class I/II profile in Brazilian pemphigus patients.

    Science.gov (United States)

    Franco Brochado, Maria José; Nascimento, Daniela Francisca; Saloum Deghaide, Neifi Hassan; Donadi, Eduardo Antonio; Roselino, Ana Maria

    2016-09-01

    Pemphigus are blistering autoimmune diseases related with genetic and environmental factors. Here we describe HLA genotyping in pemphigus patients. First, we review the HLA class I/II data on pemphigus reported in Brazilian samples and then present the HLA class I (-A, -B, -C) and class II (-DRB1, -DQA1, -DQB1) alleles related to susceptibility/resistance to pemphigus by comparing 86 patients with pemphigus foliaceus, 83 patients with pemphigus vulgaris, and 1592 controls from the northeastern region of the state of São Paulo, Southeastern Brazil. The data presented here are related to the manuscript "Differential HLA class I and class II associations in Pemphigus Foliaceus and Pemphigus Vulgaris patients from a prevalent Southeastern Brazilian region" Brochado et al. (2016) [1]. PMID:27331116

  10. Towards a strategy of reliable fusion first-wall design

    International Nuclear Information System (INIS)

    Fusion first walls are subject to a large number of possible failure mechanisms, including erosion due to sputtering, arcing, blistering and vaporization and crack growth due to thermal and magnetic stresses. Each of these failure mechanisms is poorly characterized and has the potential of being severe. A strategy for designing reliably in the face of great uncertainty is discussed. Topological features beneficial to reactor availability are identified. The integration of limiter pumping with rf wave launching is discussed, as a means of simplifying reactor design. The concept of a sewer limiter is introduced, as a possible long-life limiter topology. The concept of flexible armor is discussed, as a means of extending maximum life

  11. The T-box transcription factor Midline regulates wing development by repressing wingless and hedgehog in Drosophila.

    Science.gov (United States)

    Fu, Chong-Lei; Wang, Xian-Feng; Cheng, Qian; Wang, Dan; Hirose, Susumu; Liu, Qing-Xin

    2016-01-01

    Wingless (Wg) and Hedgehog (Hh) signaling pathways are key players in animal development. However, regulation of the expression of wg and hh are not well understood. Here, we show that Midline (Mid), an evolutionarily conserved transcription factor, expresses in the wing disc of Drosophila and plays a vital role in wing development. Loss or knock down of mid in the wing disc induced hyper-expression of wingless (wg) and yielded cocked and non-flat wings. Over-expression of mid in the wing disc markedly repressed the expression of wg, DE-Cadherin (DE-Cad) and armadillo (arm), and resulted in a small and blistered wing. In addition, a reduction in the dose of mid enhanced phenotypes of a gain-of-function mutant of hedgehog (hh). We also observed repression of hh upon overexpression of mid in the wing disc. Taken together, we propose that Mid regulates wing development by repressing wg and hh in Drosophila. PMID:27301278

  12. Characterization of spherical domains at the polystyrene thin film–water interface

    Science.gov (United States)

    Ahmad, Khurshid; Zhao, Xuezeng; Hussain, Danish

    2016-01-01

    Summary Spherical domains that readily form at the polystyrene (PS)–water interface were studied and characterized using atomic force microscopy (AFM). The study showed that these domains have similar characteristics to micro- and nanobubbles, such as a spherical shape, smaller contact angle, low line tension, and they exhibit phase contrast and the coalescence phenomenon. However, their insensitivity to lateral force, absence of long-range hydrophobic attraction, and the presence of possible contaminants and scratches on these domains suggested that these objects are most likely blisters formed by the stretched PS film. Furthermore, the analysis of the PS film before and after contact with water suggested that the film stretches and deforms after being exposed to water. The permeation of water at the PS–silicon interface, caused by osmosis or defects present on the film, can be a reasonable explanation for the nucleation of these spherical domains. PMID:27335748

  13. Pellagroid Dermatitis in an Alcoholic and Hypothyroid Patient. A Case Report

    Directory of Open Access Journals (Sweden)

    Graciela Cabrera Acea

    2015-06-01

    Full Text Available Pellagroid dermatitis is a condition resulting from the deficiency of niacin and/or tryptophan that causes dermatological, gastrointestinal and neurological symptoms. It is very rare in our country, a fact that motivated us to present this case. The patient was a 49-year-old white man diagnosed with pellagroid dermatitis. He also suffered from hypothyroidism, alcohol dependence and had poor eating habits due to his frequent drinking. He had a medical history of hypertension and presented cutaneous manifestations of pellagroid dermatitis. The erythematous and scaly manifestations were located on the back of both arms. They resembled scalded skin; some had an ulcerated and blistered center and were similar to those in the neck, deltoid region and other sites exposed to sunlight. The lesions were permanently cured after vitamin therapy and skin protection from solar radiation.

  14. Growth, straightness and survival at age 32 in a Pinus strobus x P. wallichiana F1 hybrid population (Experiment 1

    Directory of Open Access Journals (Sweden)

    Ioan Blada

    2013-11-01

    Full Text Available An interspecific factorial hybridization was made, where seven P. strobus female parent trees were mated to four P. wallichiana male trees, in order to transfer genes for resistance to blister-rust from the blue pine to the F1 hybrids. The hybrid seedlings and the open pollinated families of the two parents were three times consecutivelyinoculated with blister-rust. The six years old seedlings were transplanted in the field in the so called Văliug Experiment 1. The experimental design consisted in 28 hybrid full-sib families and two open pollinated families of the parent species. Plantation took place in a complete randomized block design with three blocks, with 6 to 12 seedlings per linear plot and 3 x 3 m spacing. This paper is based on the measurements made at age 32, just after the first thinning. Diameter at breast height, tree height, tree volume, stem straightness and tree survival were the five measured traits. According to ANOVA, significant (p < 0.05 and highly significant (p< 0.001 differences for diameter at breast height, tree height, volume growth rate, stem straightness and tree survival were detected between hybrid families. Highly significant (p < 0.01, p < 0.001 differences were found between eastern white pine female parents for survival, stem straightness and all growth traits, suggestingthat nuclear additive genes are controlling the respective characters. Consequently, high survival or genetic resistance to blister-rust can be found within the P. strobus species even though it is highly susceptible to this pathogen. The P. wallichiana parents exhibited significant additive effects on straightness and breast height diameter, but no significance in height, volume, and tree survival. The contribution of the additive variance to the phenotypic one was 65.7% for diameter, 58.6% for height, 50.5% for volume, 70.4% for stem straightness and 73.4% for tree survival. Hence, additive variance should be employed in a breeding program

  15. Successful use of ketamin combined with remifentanil in two patients with epidermolysis bullosa

    Directory of Open Access Journals (Sweden)

    İlker Öngüç Aycan

    2012-09-01

    Full Text Available Epidermolysis Bullosa (EB is characterized by the presenceof extremely fragile skin and painful blister formationsin the skin. We present the anesthetic managementof 15-years-old, twin sisters with EB during the syndactylyoperation. Premedication was done with 0.5 mg/kg nasalmidazolam. Anesthesia induction was performed withsevoflurane without touching the facial masks to the skin.After intravenous 2 mg/kg ketamine and 50 μg fentanyl,anesthesia was maintained by intravenous 1 mg/kg/hrketamine and 0.1 μg/kg/min remifentanil. We injected 2mg intravenous morphine for postoperative pain control.Ketamine with remifentanil seems to be effective, simple,and safe for airway management and practical way toprovide general anesthesia and analgesia of EB. J ClinExp Invest 2012; 3 (3: 395-397Key words: Epidermolysis bullosa, ketamin, remifentanil,anesthesia

  16. Childhood epidermolysis bullosa acquisita: Confirmation of diagnosis by skin deficient in Type VII Collagen, enzyme-linked immunosorbent assay, and immunoblotting

    Directory of Open Access Journals (Sweden)

    Nupur Goyal

    2016-01-01

    Full Text Available Epidermolysis bullosa acquisita (EBA is an acquired subepidermal bullous disorder characterized by autoantibodies against Type VII collagen. It usually affects adults; childhood EBA is rare. We describe a 10-year-old girl presenting with recurrent tense blisters predominantly on legs, dorsa of hands and feet accompanied by oral erosions since the age of 5 years. Direct immunofluorescence (IF microscopy showed linear deposition of IgG and C3 along the basement membrane zone (BMZ; indirect IF microscopy on salt-split skin revealed staining of IgG to the dermal side of the split. The patient's serum did not show BMZ staining in recessive dystrophic epidermolysis bullosa skin deficient for Type VII collagen, thus confirming autoantibody reactivity against Type VII collagen. Circulating antibodies against the immunodominant noncollagenous 1 domain of Type VII collagen were detected by ELISA and immunoblotting studies. The patient was treated with oral corticosteroids and dapsone with good improvement.

  17. Childhood Epidermolysis Bullosa Acquisita: Confirmation of Diagnosis by Skin Deficient in Type VII Collagen, Enzyme-linked Immunosorbent Assay, and Immunoblotting

    Science.gov (United States)

    Goyal, Nupur; Rao, Raghavendra; Balachandran, C; Pai, Sathish; Bhogal, Balbir S; Schmidt, Enno; Zillikens, Detlef

    2016-01-01

    Epidermolysis bullosa acquisita (EBA) is an acquired subepidermal bullous disorder characterized by autoantibodies against Type VII collagen. It usually affects adults; childhood EBA is rare. We describe a 10-year-old girl presenting with recurrent tense blisters predominantly on legs, dorsa of hands and feet accompanied by oral erosions since the age of 5 years. Direct immunofluorescence (IF) microscopy showed linear deposition of IgG and C3 along the basement membrane zone (BMZ); indirect IF microscopy on salt-split skin revealed staining of IgG to the dermal side of the split. The patient's serum did not show BMZ staining in recessive dystrophic epidermolysis bullosa skin deficient for Type VII collagen, thus confirming autoantibody reactivity against Type VII collagen. Circulating antibodies against the immunodominant noncollagenous 1 domain of Type VII collagen were detected by ELISA and immunoblotting studies. The patient was treated with oral corticosteroids and dapsone with good improvement. PMID:27293257

  18. Development of a disease registry for autoimmune bullous diseases: initial analysis of the pemphigus vulgaris subset.

    Science.gov (United States)

    Shah, Amit Aakash; Seiffert-Sinha, Kristina; Sirois, David; Werth, Victoria P; Rengarajan, Badri; Zrnchik, William; Attwood, Kristopher; Sinha, Animesh A

    2015-01-01

    Pemphigus vulgaris (PV) is a rare, potentially life threatening, autoimmune blistering skin disease. The International Pemphigus and Pemphigoid Foundation (IPPF) has recently developed a disease registry with the aim to enhance our understanding of autoimmune bullous diseases with the long-term goal of acquiring information to improve patient care. Patients were recruited to the IPPF disease registry through direct mail, e-mail, advertisements, and articles in the IPPF-quarterly, -website, -Facebook webpage, and IPPF Peer Health Coaches to complete a 38-question survey. We present here the initial analysis of detailed clinical information collected on 393 PV patients. We report previously unrecognized gender differences in terms of lesion location, autoimmune comorbidity, and delay in diagnosis. The IPPF disease registry serves as a useful resource and guide for future clinical investigation. PMID:24691863

  19. Plasma exposure of different tungsten grades with plasma accelerators under ITER-relevant conditions

    International Nuclear Information System (INIS)

    This paper presents the results of tungsten irradiation experiments performed with three plasma facilities: the QSPA Kh-50 quasi-steady-state plasma accelerator, the PPA pulsed plasma gun and the magneto-plasma compressor. Targets made of different kinds of tungsten (sintered, rolled and deformed) were irradiated with powerful plasma streams at heat fluxes relevant to edge-localized modes in ITER. The irradiated targets were analyzed and two different meshes of cracks were identified. It has been shown that the major cracks do not depend on the tungsten grade. This has been attributed to ductile-to-brittle transition effects. Meshes of inter-granular micro-cracks were detected for energy loads above the melting threshold and these were probably caused by the re-solidification process. The blister-like and cellular-like structures were observed on sample surfaces exposed to helium and hydrogen plasmas. (paper)

  20. Effects of Low Energy and High Flux Helium/Hydrogen Plasma Irradiation on Tungsten as Plasma Facing Material

    Institute of Scientific and Technical Information of China (English)

    Ye Minyou

    2005-01-01

    The High-Z material tungsten (W) has been considered as a plasma facing material in the divertor region of ITER (International Thermonuclear Experimental Reactor). In ITER, the divertor is expected to operate under high particle fluxes (> 1023 m-2s-1) from the plasma as well as from intrinsic impurities with a very low energy (< 200 eV). During the past dacade, the effects of plasma irradiation on tungsten have been studied extensively as functions of the ion energy,fluence and surface temperature in the burning plasma conditions. In this paper, recent results concerning blister and bubble formations on the tungsten surface under low energy (< 100 eV) and high flux (> 1021 m-2s-1) He/H plasma irradiation are reviewed to gain a better understanding of the performance of tungsten as a plasma facing material under the burning plasma conditions.