Management efficiency improvement promotion of SS; SS no unei koritsuka sokushin

Energy Technology Data Exchange (ETDEWEB)

NONE

1999-08-01

Full amount fund petroleum product marketer and Sumisyo petroleum of Sumitomo accelerate management efficiency improvement of service station (SS). National about 300 places have been developed in within the year Within SS, it aims at break-even point achievement of gasoline, coarse advantage 10 yen per light oil of 1 liter in 84 all tied SS stores. SS which has realized the system of 10 yen in the current is whole about 50%. But, by doing personnel configurations and operational procedures, that they reexamine the balance management, etc. in half remaining SS 12 yen-13 yen; the efficiency improvement is done thoroughly. (translated by NEDO)

Determinação da concentração de beta-glucano em cogumelo Agaricus blazei Murill por método enzimático Determination of beta-glucan concentration in Agaricus blazei Murill mushroom by enzymatic method

Directory of Open Access Journals (Sweden)

Yong K. Park

2003-12-01

Full Text Available Cogumelos comestíveis contêm importantes propriedades funcionais. Em particular, beta-glucanos, homo- e hetero-glucanos com ligações glicosídicas beta(1->3, beta(1->4 e beta(1->6, supostamente responsáveis por algumas propriedades benéficas à saúde humana, como atividade imunomodulatória, antioxidante, antiinflamatória e anticancerígena. Neste trabalho, a quantidade de beta-glucano presente em cogumelo Agaricus blazei Murill, coletados de três diferentes locais, foi analisada utilizando-se método enzimático. As amostras (em base seca foram tratadas com alfa-amilase, protease bacteriana e com glicoamilase fúngica. beta-glucano foi quantificado após hidrólise ácida e determinação da glicose liberada. Foi verificado que amostras de A. blazei Murill cultivadas em estufas apresentaram menor concentração de b-glucano (8,4±0,9 e 7,6±2,8g/100g quando comparado com amostras cultivadas em campo aberto (10,1±2,1g/100g. Observou-se ainda que, mesmo sendo cultivado em condições semelhantes, porém em locais diferentes, as amostras apresentaram diferenças significativas (7,6±2,8 e 8,4±0,9g/100g.Edible mushrooms contains a very interesting functional properties. Among them, the beta-glucans, polysaccharides with beta-1,3; b-1,4 and beta-1,6glucosidic linkages, they are responsible to a series of properties to human health, such as immunomodulatory, antioxidant, antiinflammatory and, antitumoral activities. In the present work, the Agaricus blazei Murill beta-glucan concentrations from three locations, were determined through the enzymatic method. Samples were treated with alpha-amylase, bacterial protease and fungal glucoamylase. beta-glucans were quantified after acid hydrolysis and, the glucose determined for spectrophotometric method. It was verified that samples cultivated inside stoves presented smaller beta-glucan concentration (8.4±0.9 and 7.6±2.8g/100g, when compared with samples cultivated in open field (10.1±2.1g/100

19 August 2013 - German Member of the Parliament of North Rhine-Westphalia M.-S. Abel MdL visiting the ATLAS experimental area with Senior physicist C. Rembser and German members of the collaboration B. Heinemann with G. Gaycken and D. Hirschbuehl.

CERN Multimedia

Anna Pantelia

2013-01-01

19 August 2013 - German Member of the Parliament of North Rhine-Westphalia M.-S. Abel MdL visiting the ATLAS experimental area with Senior physicist C. Rembser and German members of the collaboration B. Heinemann with G. Gaycken and D. Hirschbuehl.

DSCu/SS joining techniques development and testing

International Nuclear Information System (INIS)

Sato, Satoshi; Hatano, Toshihisa; Furuya, Kazuyuki; Kuroda, Toshimasa; Enoeda, Mikio; Takatsu, Hideyuki

1998-01-01

Joining techniques of alumina dispersion strengthened copper alloy (DSCu) and type 316L stainless steel (SS) has been investigated aiming at applying to the fabrication of the ITER first wall/blanket. As the joining method, Hot Isostatic Pressing (HIP) of solid plates and/or blocks has been pursued. By a screening test including HIP temperatures of 980-1050degC, it was concluded that the HIP temperature of 1050degC would be optimum for the simultaneous HIPping of DSCu/DSCu, DSCu/SS and SS/SS. With DSCu/SS joint specimens HIPped at 1050degC, tensile, impact, fatigue, crack propagation, and fracture toughness tests were performed as well as mechanical test of structural model with one SS circular tube embedded. Typically, the properties of the joints were almost the same as those of DSCu or SS base metal with the same heat treatment of the HIP process, thus good joints were obtained, though parts of properties were decreased at elevated test temperature. Typical results of the mechanical test of structural mode indicated that a crack initiated at the inner surface of the SS tube under cyclic operation, and the lifetime of the first wall structure could be evaluated by existing SS fatigue data. Two HIPped first wall panel mock-ups were successfully fabricated with built-in coolant tubes: one was 300 mm long and the other 800 mm long. The former was thermo-mechanically tested with high heat fluxes corresponding to the ITER operation conditions. The mock-up showed good heat removal performance during the high heat flux tests. In addition, there were no cracks and delaminations found at HIPped interfaces by microscopic observation after all tests. Ultrasonic testing have been tried as a non-destructive examination method, and detectable defect size at SS/SS, DSCu/DSCu and DSCu/SS joint interfaces were estimated. (author)

The Medicinal Mushroom Agaricus blazei Murrill: Review of Literature and Pharmaco-Toxicological Problems

Directory of Open Access Journals (Sweden)

F. Firenzuoli

2008-01-01

Full Text Available Agaricus blazei Murrill (ABM popularly known as ‘Cogumelo do Sol’ in Brazil, or ‘Himematsutake’ in Japan, is a mushroom native to Brazil, and widely cultivated in Japan for its medicinal uses, so it is now considered as one of the most important edible and culinary-medicinal biotechnological species. It was traditionally used to treat many common diseases like atherosclerosis, hepatitis, hyperlipidemia, diabetes, dermatitis and cancer. In vitro and in vivo ABM has shown immunomodulatory and antimutagenic properties, although the biological pathways and chemical substances involved in its pharmacological activities are still not clear. The polysaccharides phytocomplex is thought to be responsible for its immunostimulant and antitumor properties, probably through an opsonizing biochemical pathway. Clinical studies are positive confirmations, but we are still at the beginning, and there are perplexing concerns especially relative to the content of agaritine. Argantine is a well-known carcinogenic and toxic substance in animals, that must be completely and fully evaluated.

23 July - Italian Director-General for Prevention G. Ruocco and Director-General for European and International Relations Ministry of Health D. Roderigo visiting the ATLAS experimental cavern with ATLAS Deputy Spokesperson B. Heinemann. Life Sciences Section M. Cirilli and Life Sciences Adviser M. Dosanjh present.

CERN Multimedia

Anna Pantelia

2013-01-01

23 July - Italian Director-General for Prevention G. Ruocco and Director-General for European and International Relations Ministry of Health D. Roderigo visiting the ATLAS experimental cavern with ATLAS Deputy Spokesperson B. Heinemann. Life Sciences Section M. Cirilli and Life Sciences Adviser M. Dosanjh present.

AGARICUS BLAZEI MURRILL MUSHROOM COMPOST STUDY ANAEROBIC AND AEROBIC PHASES

Directory of Open Access Journals (Sweden)

Sándor Rózsa

2017-12-01

Full Text Available Compost for the production of Agaricus blazei Murrill mushrooms, is produced from wheat straw, straw-bedded horse manure, chicken manure and gypsum. The substrate is made in two processes called Phase I (anaerobic and Phase II (aerobic. Phase I includes mixing and moistening of the ingredients and a period of uncontrolled self-heating where temperatures will rise to 80ºC. Phase II starts with a pasteurization period of 8h at 56-60ºC and continues with a conditioning period at 45ºC for up to 7 days until volatile NH3 has been cleared from the process by air. Quality parameters for compost cannot be established directly. Moisture and nitrogen contents and pH can be adjusted at the start of Phase I, but the values will be affected during processing. In this paperwork, we studied the physical properties (water content, electrical conductivity and chemical composition (pH, organic matter, nitrogen, calcium, magnesium, ammonia of four recipes of compost: classical, synthetic, mixt and original. During the experience, we recorded every hour the compost and the air temperature and the air relative humidity. The highest yield was obtained on synthetic compost with 42 kg mushrooms on 100 kg of compost.

Pitting Corrosion Behavior of 304 SS and 316 SS Alloys in Aqueous Chloride and Bromide Solutions

Directory of Open Access Journals (Sweden)

Ibtehal Kareem Shakir

2018-01-01

Full Text Available The importance of the present work falls on the pitting corrosion behavior investigation of 304 SS and 316 SS alloys in 3.5 wt% of aqueous solution bearing with chloride and bromide anion at different solutions temperature range starting from (20-50oC due to the pitting corrosion tremendous effect on the economic, safety and materials loss due to leakage. The impact of solution temperatures on the pitting corrosion resistance at 3.5wt% (NaCl and NaBr solutions for the 304 SS and 316 SS has been investigated utilizing the cyclic polarization techniques at the potential range -400 to1000 mV vs. SCE at 40 mV/sec scan rate followed by the surface characterization employing Scanning Electron Microscope. The results show that a significant decline in the pitting corrosion potential Ep values of both stainless steel alloys in chloride and bromide solution during temperature increase attributed to the pitting corrosion potential decreased arises from the modification of the passive film properties. The surface examination using optical microscope and scanning electron microscope prove the occurring of higher pitting density over 304 SS in chloride solution than that observed in bromide solution with a non-circular lacy cover pitfall out at the center and falls inside the pits hall in comparison to the isolated circular lacy cover pit formed on 316 SS in 3.5wt% NaBr solution at 50 oC.

The Effects of Agaricus blazei Murill Polysaccharides on Cadmium-Induced Apoptosis and the TLR4 Signaling Pathway of Peripheral Blood Lymphocytes in Chicken.

Science.gov (United States)

Liu, Wenjing; Ge, Ming; Hu, Xuequan; Lv, Ai; Ma, Dexing; Huang, Xiaodan; Zhang, Ruili

2017-11-01

In this study, we investigated the effects of Agaricus blazei Murill polysaccharides (ABP) on cadmium (Cd)-induced apoptosis and the TLR4 signaling pathway of chicken peripheral blood lymphocytes (PBLs). Seven-day-old healthy chickens were randomly divided into four groups, and each group contained 20 males. The cadmium-supplemented diet group (Cd group) was fed daily with full feed that contained 140 mg cadmium chloride (CdCl 2 )/kg and 0.2 mL saline. The A. blazei Murill polysaccharide diet group (ABP group) was fed daily with full feed with 0.2 mL ABP solution (30 mg/mL) by oral gavage. The cadmium-supplemented plus A. blazei Murill polysaccharide diet group (Cd + ABP group) was fed daily with full feed containing 140 mg CdCl 2 /kg and 0.2 mL ABP solution (30 mg/mL) by gavage. The control group was fed daily with full feed with 0.2 mL saline per day. We measured the apoptosis rate and messenger RNA (mRNA) levels of apoptosis genes (caspase-3, Bax, and Bcl-2), the mRNA levels of TLR4 and TLR4 signaling pathway-related factors (MyD88, TRIF, NF-κB, and IRF3), the TLR4 protein expression, and the concentrations of inflammatory cytokines (IL-1β, IL-6, and TNF-α) in chicken PBLs. The results showed that the PBL apoptosis rate was significantly increased, the mRNA levels of caspase-3 and Bax were significantly increased, while that of Bcl-2 was significantly reduced. The Bax/Bcl-2 ratio was significantly increased in the Cd group at 20, 40, and 60 days after treatment compared with that in the control group. After treatment with ABP, the above changes were clearly suppressed. At the same time, ABP reduced the concentrations of IL-1β, IL-6, and TNF-α induced by Cd. We also found that ABP inhibited the TLR4 mRNA level and protein expression and inhibited the mRNA levels of MyD88, TRIF, NF-κB, and IRF3. The results demonstrated that Cd could induce apoptosis, activate the TLR4 signaling pathway, and induce the expression of inflammatory cytokines in

On the dopplars SS433

International Nuclear Information System (INIS)

Fang Li Zhi; Riffini, R.

1979-07-01

A new family of X-ray sources has recently been proposed including SS433. The general relativistic formula to fit the frequency dependence of Hsub(α) lines in SS433 as a function of the phase is presented here. Particularly relevant for the verification of the model are the observations at the phase of minimum shift. (author)

Mechanochemical regulations of RPA's binding to ssDNA

Science.gov (United States)

Chen, Jin; Le, Shimin; Basu, Anindita; Chazin, Walter J.; Yan, Jie

2015-03-01

Replication protein A (RPA) is a ubiquitous eukaryotic single-stranded DNA (ssDNA) binding protein that serves to protect ssDNA from degradation and annealing, and as a template for recruitment of many downstream factors in virtually all DNA transactions in cell. During many of these transactions, DNA is tethered and is likely subject to force. Previous studies of RPA's binding behavior on ssDNA were conducted in the absence of force; therefore the RPA-ssDNA conformations regulated by force remain unclear. Here, using a combination of atomic force microscopy imaging and mechanical manipulation of single ssDNA tethers, we show that force mediates a switch of the RPA bound ssDNA from amorphous aggregation to a much more regular extended conformation. Further, we found an interesting non-monotonic dependence of the binding affinity on monovalent salt concentration in the presence of force. In addition, we discovered that zinc in micromolar concentrations drives ssDNA to a unique, highly stiff and more compact state. These results provide new mechanochemical insights into the influences and the mechanisms of action of RPA on large single ssDNA.

The SS-SCR Scheme for Dynamic Spectrum Access

Directory of Open Access Journals (Sweden)

Vinay Thumar

2012-01-01

Full Text Available We integrate the two models of Cognitive Radio (CR, namely, the conventional Sense-and-Scavenge (SS Model and Symbiotic Cooperative Relaying (SCR. The resultant scheme, called SS-SCR, improves the efficiency of spectrum usage and reliability of the transmission links. SS-SCR is enabled by a suitable cross-layer optimization problem in a multihop multichannel CR network. Its performance is compared for different PU activity patterns with those schemes which consider SS and SCR separately and perform disjoint resource allocation. Simulation results depict the effectiveness of the proposed SS-SCR scheme. We also indicate the usefulness of cloud computing for a practical deployment of the scheme.

Relativistic jets in SS 433

International Nuclear Information System (INIS)

Margon, B.

1982-01-01

The most unusual characteristic of the star SS 433 emerged in the late 1970's when a series of optical spectra showed intense, broad optical emission lines whose profiles and wavelengths changed drastically from night to night. These features are interpreted as strong Doppler-shifted Balmer and HeI lines. The modulation of the Doppler shifts are observed as being cyclic with a period of about 164 days. It was hypothesized that these phenomena were caused by two collimated, colinear, jets which were ejecting in opposite directions from SS 433. Most authors believe that velocity variations of the emission lines are caused by a cyclic rotation of jet axis inclined to line of sight. This rotation being the result of precession, which leads one to suspect SS 433 as a member of a close binary system. This hypothesis has been confirmed from recent optical, radio, and x-ray observations which are discussed in the article. The combination of optical and radio observations of SS 433, described in the article, gives an accurate measure of the Kinematics of the system and some confidence that the Kinematic equations are understood. However, the specific physical processes of this ejection are poorly understood. Some theoretical difficulties regarding this are given

Effect of 60Co γ-rays on growth of Agaricus blazei murill hypha and its cell shape structure

International Nuclear Information System (INIS)

Weng Boqi; Jiang Zhihe; Lin Yong; Huang Tingjun; Xiao Shuxia

2003-01-01

The effect of 60 Co γ-rays at different dosage on growth, tangle and structure of cell shape of Agarius blazei Murill (Abm) hypha were studied. The experiment results showed that the cell wall of treatment Abm hypha was thicker than that of the control, huge separation between cytoplasm and cell wall of Abm hypha appeared when Abm hypha was irradiated at the low dosage (0.2-0.5 kGy). The cell wall became thin and small separation between cytoplasm and cell wall appeared with the increase of the irradiation dosage. The growth of Abm hypha became slow and scare by irradiation with higher dosage. The growth and tangle of hypha could be improved and the yield of fruit body increased by 34.8% when irradiated with low dosage

19 July 2013 - Chairman of the Policy Committee, European Cancer Organisation, President, European Association for Cancer Research E. Celis visiting the ATLAS experimental cavern with ATLAS Collaboration Deputy Spokesperson, B. Heinemann and signing the Guest Book with Director for Accelerators and Technology S. Myers. Life Sciences Adviser M. Dosanjh present.

CERN Multimedia

Anna Pantelia

2013-01-01

19 July 2013 - Chairman of the Policy Committee, European Cancer Organisation, President, European Association for Cancer Research E. Celis visiting the ATLAS experimental cavern with ATLAS Collaboration Deputy Spokesperson, B. Heinemann and signing the Guest Book with Director for Accelerators and Technology S. Myers. Life Sciences Adviser M. Dosanjh present.

Anti-Inflammatory Activity of Water-Soluble Polysaccharide of Agaricus blazei Murill on Ovariectomized Osteopenic Rats

Directory of Open Access Journals (Sweden)

Peng Wang

2013-01-01

Full Text Available In the present study, we investigated the anti-inflammatory activity of water-soluble polysaccharide of Agaricus blazei Murill (WSP-AbM on ovariectomized osteopenic rats. The rats were administered orally WSP-AbM (200 mg/kg BW for 8 weeks. Subsequent serum maleic dialdehyde (MDA level, total antioxidant status (TAOS, nuclear factor kappa B (NF-κB level, polymorphonuclear (PMN cells level, interleukin-1β (IL-1β level, inducible nitric oxide synthase (iNOS level, tumor necrosis factor-α (TNF-α level, adhesion molecule (ICAM-1, and cyclooxygenase-2 (COX-2 were determined by enzyme linked immunosorbent assay (ELISA and immunohistochemistry, respectively. WSP-AbM administration markedly (P<0.05 decreased serum IL-1β and TNF-α levels and the expressions of ICAM-1, COX-2, and iNOS NF-κB compared with OVX rats. WSP-AbM administration alsomarkedly (P<0.05 decreased PMN infiltration. In conclusion, we observed that WSP-AbM supplementation had anti-inflammatory effects in a model of osteoporosis disease.

Autoalan työssäoppiminen : Case: Autoalan merkkiliikkeet

OpenAIRE

Kalliomäki, Marko

2013-01-01

Tutkimuksen tavoitteena oli selvittää Jyväskylän ammattiopiston autoalan työssäoppimista Jyväskylän autoalan merkkiliikkeissä työnantajien näkökulmasta. Työssä selvitettiin yhteistyön nykytilanne ja millaisia ongelmia yhteistyössä koetaan olevan. Mukaan otettiin myös oppilaitoksen näkemyksiä ja kokemuksia yhteistyöstä autoalan merkkiliikkeiden kanssa työssäoppimisesta. Tutkimustulosten perusteella rakennettiin kehittämissuunnitelma yhteistyön kehittämisen pohjaksi. Tutkimusaineisto saatii...

Advanced drug delivery nanosystems (aDDnSs): a mini-review.

Science.gov (United States)

Demetzos, Costas; Pippa, Natassa

2014-06-01

Significant progress has been made in nanoscale drugs and delivery systems employing diverse chemical formulations to facilitate the rate of drug delivery and to improve its pharmacokinetics. Biocompatible nanomaterials have been used as biological markers, contrast agents for imaging, healthcare products, pharmaceuticals, drug-delivery systems as well as in detection, diagnosis and treatment of various types of diseases. The classification of drug delivery nanosystems (DDnSs) is a crucial issue and fundamental efforts on this subject are missing from the literature. This article deals with the classification of DDnSs with a modulatory controlled release profile (MCR) denoted as modulatory controlled release nanosystems (MCRnSs). Conventional (c) and advanced (a) DDnSs are denoted by the acronyms cDDnSs and aDDnSs, and can be composed of a single or more than one biomaterials, respectively. The classification was based on their characteristics such as: surface functionality (f), the nature of biomaterials used and the kind of interactions between biomaterials. The aDDnSs can be classified as hybridic (Hy-) or chimeric (Chi-) based on the nature - same or different respectively - of biomaterials and inorganic materials used. The nature of the elements used for producing advanced biomaterials is of great importance and medicinal chemistry contributes effectively to the production of aDDnSs.

Construction, characterization and evaluation of the protective efficacy of the Streptococcus suis double mutant strain ΔSsPep/ΔSsPspC as a live vaccine candidate in mice.

Science.gov (United States)

Hu, Jin; You, Wujin; Wang, Bin; Hu, Xueying; Tan, Chen; Liu, Jinlin; Chen, Huanchun; Bei, Weicheng

2015-01-01

Streptococcus suis serotype 2 (S. suis 2) causes sepsis and meningitis in piglets and humans, and results in one of the most serious bacterial diseases affecting the production of commercial pigs around the world. Due to the failure of the current inactivated vaccine to protect against the disease, development of a new attenuated live vaccine against S. suis 2 by deleting essential virulence factors is urgently needed. We have previously reported the construction and characterization of an SsPep single gene deletion mutant strain ΔSsPep based on S. suis 2. Our previous results have shown that SsPep plays a critical role in the pathogenesis of S. suis 2. In this study, a precisely defined double-deletion mutant ΔSsPep/ΔSsPspC of S. suis 2 without antibiotic-resistance markers was constructed based on ΔSsPep, and the levels of virulence of the wild-type (WT) and ΔSsPep/ΔSsPspC were compared in a mouse experimental infection model. We demonstrated that the double mutant ΔSsPep/ΔSsPspC was less virulent than the WT, and could induce a noticeable antibody response. Analysis of IgG subclasses (IgG1 and IgG2a) indicated that both Th1 and Th2 responses were induced by ΔSsPep/ΔSsPspC, although the IgG2a (Th1) response predominated over the IgG1 (Th2) response. Moreover, ΔSsPep/ΔSsPspC could confer 90% protective efficacy against challenge with a lethal dose of fully virulent S. suis 2. Taken together, these data demonstrate that ΔSsPep/ΔSsPspC can be used as an effective live vaccine and provide a novel strategy against infection of S. suis 2. Copyright © 2014 Elsevier GmbH. All rights reserved.

Sosiaalisen median rooli kunnan viestinnässä

OpenAIRE

Selkämaa, Kati

2016-01-01

Opinnäytetyön tavoitteena oli selvittää sosiaalisen median roolia kunnan viestinnässä sekä tutkia, miten sosiaalista mediaa hyödynnetään kuntien viestinnässä. Teoriaosuudessa tarkasteltiin sosiaalista mediaa, tutustuttiin sen tunnetuimpiin sovelluksiin sekä perehdyttiin kuntien viestintään yleisesti. Työssä tarkasteltiin myös kuntien viestintään vaikuttavia ja sitä sääteleviä lakeja. Kuntien sosiaalisen median käyttöön tutustuttiin Kuntaliiton tekemän viestintätutkimuksen tulosten pohjalt...

Phase I Clinical Study of the Dietary Supplement, Agaricus blazei Murill, in Cancer Patients in Remission

Directory of Open Access Journals (Sweden)

Satoshi Ohno

2011-01-01

Full Text Available Although many cancer patients use complementary and alternative medicine, including Agaricus blazei Murill (ABM, safety is not yet well understood. Cancer survivors took 1.8, 3.6, or 5.4 g ABM granulated powder (Kyowa Wellness Co., Ltd., Tokyo, Japan per day orally for 6 months. Adverse events were defined by subjective/objective symptoms and laboratory data according to the National Cancer Institute Common Terminology Criteria for Adverse Events version 3.0 (NCI-CTCAE v3.0. Seventy-eight patients were assessed for safety of ABM (30/24/24 subjects at 1/2/3 packs per day, resp.. Adverse events were observed in 9 patients (12%. Most were digestive in nature such as nausea and diarrhea, and one patient developed a liver dysfunction-related food allergy, drug lymphocyte product. However, none of these adverse events occurred in a dose-dependent manner. This study shows that ABM does not cause problems in most patients within laboratory parameters at the dosages tested over 6 months. This trial supports previous evidence that the ABM product is generally safe, excluding possible allergic reaction.

CellSs: Scheduling Techniques to Better Exploit Memory Hierarchy

Directory of Open Access Journals (Sweden)

Pieter Bellens

2009-01-01

Full Text Available Cell Superscalar's (CellSs main goal is to provide a simple, flexible and easy programming approach for the Cell Broadband Engine (Cell/B.E. that automatically exploits the inherent concurrency of the applications at a task level. The CellSs environment is based on a source-to-source compiler that translates annotated C or Fortran code and a runtime library tailored for the Cell/B.E. that takes care of the concurrent execution of the application. The first efforts for task scheduling in CellSs derived from very simple heuristics. This paper presents new scheduling techniques that have been developed for CellSs for the purpose of improving an application's performance. Additionally, the design of a new scheduling algorithm is detailed and the algorithm evaluated. The CellSs scheduler takes an extension of the memory hierarchy for Cell/B.E. into account, with a cache memory shared between the SPEs. All new scheduling practices have been evaluated showing better behavior of our system.

Clinical characteristics of RA patients with secondary SS and association with joint damage

OpenAIRE

Brown, Lindsay E.; Frits, Michelle L.; Iannaccone, Christine K.; Weinblatt, Michael E.; Shadick, Nancy A.; Liao, Katherine P.

2014-01-01

Objectives. Secondary SS (sSS) is a common extra-articular manifestation of RA. There are conflicting data regarding the association of sSS with worse joint damage. This study aims to characterize sSS patients in an RA cohort and study the association between sSS and joint damage.

A kinematic model for SS433

International Nuclear Information System (INIS)

Abell, G.O.; Margon, B.

1979-01-01

A model is suggested to explain the bizarre object SS433 which was first noted because of its H α emission and most recently because it is the optical counterpart of a variable radio and X-ray source and exhibits an extraordinary optical spectrum. It is considered that the radiation, with emission lines which show variable Doppler shift, is emitted by hot matter ejected by the central object at high but nearly constant velocity in oppositely directed narrow streams, possible along a magnetic axis. Rotation of the beam axis provides the observed radial velocity variations. The red and blue shifts of SS433 measured by a number of workers on 55 nights during 1978-79 and folded with a 164-d period are shown, from which it is predicted that on or about 1 July 1979 the two moving emission line systems in SS433 will briefly merge into one, similar to a previously reported episode, and that for the following 40 days the lines will separate again, but by an amount much less than previously observed. (author)

NanoRNase from Aeropyrum pernix shows nuclease activity on ssDNA and ssRNA.

Science.gov (United States)

Deng, Yong-Jie; Feng, Lei; Zhou, Huan; Xiao, Xiang; Wang, Feng-Ping; Liu, Xi-Peng

2018-05-01

In cells, degrading DNA and RNA by various nucleases is very important. These processes are strictly controlled and regulated to maintain DNA integrity and to mature or recycle various RNAs. NanoRNase (Nrn) is a 3'-exonuclease that specifically degrades nanoRNAs shorter than 5 nucleotides. Several Nrns have been identified and characterized in bacteria, mainly in Firmicutes. Archaea often grow in extreme environments and might be subjected to more damage to DNA/RNA, so DNA repair and recycling of damaged RNA are very important in archaea. There is no report on the identification and characterization of Nrn in archaea. Aeropyrum pernix encodes three potential Nrns: NrnA (Ape1437), NrnB (Ape0124), and an Nrn-like protein Ape2190. Biochemical characterization showed that only Ape0124 could degrade ssDNA and ssRNA from the 3'-end in the presence of Mn 2+ . Interestingly, unlike bacterial Nrns, Ape0124 prefers ssDNA, including short nanoDNA, and degrades nanoRNA with lower efficiency. The 3'-DNA backbone was found to be required for efficiently hydrolyzing the phosphodiester bonds. In addition, Ape0124 also degrads the 3'-overhang of double-stranded DNA. Interestingly, Ape0124 could hydrolyze pAp into AMP, which is a feature of bacterial NrnA, not NrnB. Our results indicate that Ape0124 is a novel Nrn with a combined substrate profile of bacterial NrnA and NrnB. Copyright © 2018 Elsevier B.V. All rights reserved.

Analysis of γ-ray irradiated pasteurized agaricus (Agaricus blazei Murill) by ESR in oxygen-free atmosphere

International Nuclear Information System (INIS)

Kemeya, Hiromi; Ukai, Mitsuko

2007-01-01

Agaricus (Agaricus blazei Murill) is one of a popular crude drug in Japan and East Asia countries. The crude drugs originating from natural raw products are easy to be contaminated by microorganism. Radiation method has been introduced as the sterilization technology for crude drugs. ESR has been used for the detection of radicals in irradiated drugs. Using ESR, we detected radical species in Agaricus before and after the gamma ray pasteurization. The ESR spectrum consists of a broad sextet centered at g=2.0, a singlet at same g-value and a singlet at g=4.0. We found that relaxation times of radicals in Agaricus varied by the presence of oxygen. Upon substitution of air by Ar, i.e., the absent of oxygen in the ESR sample tube, the T 1 value became 10 times larger than the presence of oxygen. Accordingly, the progressive saturation curve indicates more immediate saturation under the Ar than air atmosphere. Radicals, produced by the γ-ray irradiation to Agaricus, are very sensitive to oxygen. We concluded that ESR spectrum of irradiated Agaricus sample in the absence of oxygen is useful for the detection of dose level of γ-ray irradiation. (author)

Taloushallintoprosessien kehittäminen Iisalmen Putkiasennus Oy:ssä

OpenAIRE

Juntunen, Tiia

2015-01-01

Tämä opinnäytetyö käsittelee taloushallintoprosessien kehittämistä Iisalmen Putkiasennus Oy:ssä. Kehittämistyötä päädyttiin rajaamaan keskittyen myynti- ja ostolaskuprosessien kehittämiseen, koska niissä oli havaittu olevan eniten kehitettävää. Iisalmen Putkiasennus Oy on LVI-alalla työskentelevä perheyritys, joka on ollut toiminnassa jo yli 35 vuotta. Yritys sijaitsee Iisalmessa ja työllistää noin 50 henkilöä. Tässä opinnäytetyössä taloushallintoprosessien kehittäminen alkoi tilanteesta...

YouTube osuuskaupan markkinointiviestinnässä

OpenAIRE

Lindevall, Tytti

2014-01-01

Tämän opinnäytetyön tavoitteena on luoda toimeksiantaja Suur-Seudun Osuuskauppa SSO:lle suunnitelma YouTuben hyödyntämiseksi markkinointiviestinnässään. Työn avulla on tarkoitus löytää keino, miten SSO voisi hyödyntää YouTubea markkinointiviestinnässään ja mitä asioita tulisi huomioida omaa kanavaa perustettaessa. Tarkoituksena on tuottaa raportti mahdollisista käyttötarkoituksista sekä ohjeistus oman kanavan perustamiseen ja hallinnointiin liittyvistä asioista. Työn alkupuolella taustoite...

AtlasT4SS: a curated database for type IV secretion systems.

Science.gov (United States)

Souza, Rangel C; del Rosario Quispe Saji, Guadalupe; Costa, Maiana O C; Netto, Diogo S; Lima, Nicholas C B; Klein, Cecília C; Vasconcelos, Ana Tereza R; Nicolás, Marisa F

2012-08-09

The type IV secretion system (T4SS) can be classified as a large family of macromolecule transporter systems, divided into three recognized sub-families, according to the well-known functions. The major sub-family is the conjugation system, which allows transfer of genetic material, such as a nucleoprotein, via cell contact among bacteria. Also, the conjugation system can transfer genetic material from bacteria to eukaryotic cells; such is the case with the T-DNA transfer of Agrobacterium tumefaciens to host plant cells. The system of effector protein transport constitutes the second sub-family, and the third one corresponds to the DNA uptake/release system. Genome analyses have revealed numerous T4SS in Bacteria and Archaea. The purpose of this work was to organize, classify, and integrate the T4SS data into a single database, called AtlasT4SS - the first public database devoted exclusively to this prokaryotic secretion system. The AtlasT4SS is a manual curated database that describes a large number of proteins related to the type IV secretion system reported so far in Gram-negative and Gram-positive bacteria, as well as in Archaea. The database was created using the RDBMS MySQL and the Catalyst Framework based in the Perl programming language and using the Model-View-Controller (MVC) design pattern for Web. The current version holds a comprehensive collection of 1,617 T4SS proteins from 58 Bacteria (49 Gram-negative and 9 Gram-Positive), one Archaea and 11 plasmids. By applying the bi-directional best hit (BBH) relationship in pairwise genome comparison, it was possible to obtain a core set of 134 clusters of orthologous genes encoding T4SS proteins. In our database we present one way of classifying orthologous groups of T4SSs in a hierarchical classification scheme with three levels. The first level comprises four classes that are based on the organization of genetic determinants, shared homologies, and evolutionary relationships: (i) F-T4SS, (ii) P-T4SS, (iii

French validation of the Maslach Burnout Inventory-Student Survey (MBI-SS).

Science.gov (United States)

Faye-Dumanget, Christine; Carré, Julie; Le Borgne, Margaux; Boudoukha, Pr Abdel Halim

2017-12-01

Several international studies have been conducted on student burnout. To contribute to the clinical examination as well as research on the mental health of students, the MBI-SS (Maslach Burnout Inventory-Student Survey) has been validated and used in different countries but not in French. The aim of this study is to examine the validity of the 3-dimensional model of the French version of the MBI-SS, which is characterized by emotional "Emotional Exhaustion" (EE); "Cynicism" (CY); and low scores in "Academic Efficacy" (AE). A total of 667 university students were questioned to study the 3-dimensional structure of the French translation of the MBI-SS. The results validate the 3-dimensional structure of the MBI-SS and indicate satisfactory psychometric values. It is concluded that the MBI-SS can be used to assess burnout in French students. © 2017 John Wiley & Sons, Ltd.

OPNET simulation Signaling System No.7 (SS7) network interfaces

OpenAIRE

Ow, Kong Chung.

2000-01-01

This thesis presents an OPNET model and simulation of the Signaling System No.7 (SS7) network, which is dubbed the world's largest data communications network. The main focus of the study is to model one of its levels, the Message Transfer Part Level 3, in accordance with the ITU.T recommendation Q.704. An overview of SS7 that includes the evolution and basics of SS7 architecture is provided to familarize the reader with the topic. This includes the protocol stack, signaling points, signaling...

Bizarre spectrum of SS 433

International Nuclear Information System (INIS)

Margon, B.

1980-01-01

SS 433 is an emission-line star, a radio source, and an x-ray source centered in a supernova remnant, W50. Through observations and spectroscopic studies much has been learned of this object. The spectrum of SS 433 is characterized by Doppler-shifted emission lines of hydrogen and helium. This implies the presence of a gas. The wavelengths of these lines vary. Some of the moving lines of the spectrum are red-shifted and some are blue-shifted. Since the wavelengths of the Doppler-shifted features change rapidly with time, the velocity of both approaching gas and receding gas is changing. On any given night the average velocity is 12,000 km/sec. Also, the variations in velocity are periodic, with a 164-day period. These characteristics of the spectrum of SS 433 are explained by assuming the following hypothetical model. The object responsible for ejecting two jets in opposite directions is thought to be part of a binary system, consisting of a comparatively normal star bound in close orbit to a neutron star, which is in the process of pulling material away from the companion by virtue of its strong gravitational field. The gas streaming from the normal star forms a rotating accretion disk around the neutron star, and it is from the faces of this disk that the two jets are ejected in opposite directions. Precession of the plane of the disk is presumably what causes the axis of the jets to rotate, hence the 164-day period

Two New Sharp Ostrowski-Grüss Type Inequalities

Directory of Open Access Journals (Sweden)

Zheng Liu

2013-11-01

Full Text Available The purpose of this paper is to use a variant of the Grüss inequality to derive two new sharp Ostrowski-Grüss type inequalities related to a perturbed trapezoidal type rule and a perturbed generalized interior point rule, respectively, which provide improvements of some previous results in the literatures.

EuroPhenome and EMPReSS: online mouse phenotyping resource.

Science.gov (United States)

Mallon, Ann-Marie; Blake, Andrew; Hancock, John M

2008-01-01

EuroPhenome (http://www.europhenome.org) and EMPReSS (http://empress.har.mrc.ac.uk/) form an integrated resource to provide access to data and procedures for mouse phenotyping. EMPReSS describes 96 Standard Operating Procedures for mouse phenotyping. EuroPhenome contains data resulting from carrying out EMPReSS protocols on four inbred laboratory mouse strains. As well as web interfaces, both resources support web services to enable integration with other mouse phenotyping and functional genetics resources, and are committed to initiatives to improve integration of mouse phenotype databases. EuroPhenome will be the repository for a recently initiated effort to carry out large-scale phenotyping on a large number of knockout mouse lines (EUMODIC).

Hoitajien kokemuksia voimistavan johtamisen arvojen ilmenemisestä esimiestyössä

OpenAIRE

Karvinen, Päivi

2013-01-01

Tässä opinnäytetyössä tutkittiin hoitajien kokemuksia voimistavan johtamisen keskeisten arvojen ilmenemisessä esimiestyössä. Voimistavan johtamisen keskeisiksi arvoiksi valittiin viisi Hannele Laaksosen (2008) nimeämää arvoa: avoimuus, luottamus, arvostus, oikeudenmukaisuus ja itseohjautuvuus. Tutkimus kuvaa hoitajien kokemuksia siitä, kuinka esimies voi edistää voimistavan johtamisen arvoja. Hoitajien kokemuksia ei rajattu nykyiseen työsuhteeseen. Hoitajat kertoivat kokemuksistaan yleisesti....

Effect of alloying elements on the isothermal solidification during TLP bonding of SS 410 and SS 321 using a BNi-2 interlayer

Energy Technology Data Exchange (ETDEWEB)

Arafin, M.A. [Department of Mechanical and Industrial Engineering, Concordia University, Montreal, H3G 1M8 (Canada)], E-mail: ma_arafi@encs.concordia.ca; Medraj, M. [Department of Mechanical and Industrial Engineering, Concordia University, Montreal, H3G 1M8 (Canada)], E-mail: mmedraj@encs.concordia.ca; Turner, D.P. [Metallurgical Planning, Pratt and Whitney Canada, Longueuil, Que., J4G 1A1 (Canada)], E-mail: Daniel.P.Turner@pwc.ca; Bocher, P. [Department de Genie Mecanique, Ecole de Technologie Superieure, Montreal, H3C 1K3 (Canada)], E-mail: Philippe.Bocher@etsmtl.ca

2007-11-15

The random diffusion of solute atoms during transient liquid phase (TLP) bonding of SS 410 and 321 with nickel-based brazing filler alloy BNi-2 have been modeled using Random Walk Modeling technique. Cumulative probability distributions and probability density functions of isothermal solidification times have been calculated for different process conditions and verified with experimental data. The solubility limit of boron has been found to have decreased from 0.3 at.% at higher temperature bonding operations (1358-1394 K) because of substantial iron-rich base metal dissolution when SS 410 was used as base metals; whereas it remained unchanged for SS 321/BNi-2 combination because of high concentrations of nickel and chromium in the base metal. Silicon diffusion model, based on the EDS analysis, also predicted the isothermal solidification times reasonably well.

Features of systemic sclerosis-rheumatoid arthritis overlap syndrome (SS-RA overlap syndrome

Directory of Open Access Journals (Sweden)

O. V. Desinova

2007-01-01

Full Text Available Objective. To reveal clinico-laboratory, immunologic and immunogenetic features of systemic sclerosis-rheumatoid arthritis overlap syndrome (SS-RA.Material and methods. 32 pts with SS-RA (1 male, 31 female aged 22 to 74 years with disease onset at 18 to 69 years and disease duration from 1 to 35 years were included. Complex laboratory and instrumental examination was performed including nailfold capillaroscopy. A part of pts was also evaluated with magnetic resonance imaging of hands. Serum level of rheumatoid factor, antinuclear factor, circulating immune complexes, C-reactive protein, antibodies to cyclic citrullinated peptide (ACCP was evaluated. Genotyping of DRB1 alleles was performed.Results. Characteristic features of SS-RA were prevalence of limited skin damage, less prominent peripheral and visceral symptoms of SS, presence of anti-topoisomerase antibodies and erosive arthritis, high laboratory and immunological activity, more frequent association with DRB1*01.Conclusion. SS-RA possesses its own clinical features and can be considered as a distinct subtype of SS.

Is PiSS Alpha-1 Antitrypsin Deficiency Associated with Disease?

Science.gov (United States)

McGee, Dawn; Schwarz, Laura; McClure, Rebecca; Peterka, Lauren; Rouhani, Farshid; Brantly, Mark; Strange, Charlie

2010-01-01

Background. Alpha-1 antitrypsin deficiency (AAT) is an inherited condition that predisposes to lung and/or liver disease. Objective. The current study examined the clinical features of the PiSS genotype. Methods. Nineteen study participants (PiSS) and 29 matched control participants (PiMM) were telephone interviewed using a standardized questionnaire. Demographic features, cigarette smoking, vocation, medication history, and clinical diagnoses were compared. Statistical analysis was performed. Finally, a comprehensive literature review was performed by two investigators. Results. 12/19 (63.2%) study participants reported the presence of lung and/or liver disease compared to 12/29 (41.4%) control participants. There trended toward having a higher frequency of medication allergies in the study population (42.11% versus 20.69%). Conclusions. The PiSS genotype was associated with a similar incidence of obstructive lung disease to controls. Selective bias intrinsic in testing for AAT deficiency and the rarity of the PiSS genotype will make future study of this association dependent on population-based tests.

Validation of Robotic Surgery Simulator (RoSS).

Science.gov (United States)

Kesavadas, Thenkurussi; Stegemann, Andrew; Sathyaseelan, Gughan; Chowriappa, Ashirwad; Srimathveeravalli, Govindarajan; Seixas-Mikelus, Stéfanie; Chandrasekhar, Rameella; Wilding, Gregory; Guru, Khurshid

2011-01-01

Recent growth of daVinci Robotic Surgical System as a minimally invasive surgery tool has led to a call for better training of future surgeons. In this paper, a new virtual reality simulator, called RoSS is presented. Initial results from two studies - face and content validity, are very encouraging. 90% of the cohort of expert robotic surgeons felt that the simulator was excellent or somewhat close to the touch and feel of the daVinci console. Content validity of the simulator received 90% approval in some cases. These studies demonstrate that RoSS has the potential of becoming an important training tool for the daVinci surgical robot.

Responsiivinen web-kehitys WordPress-julkaisujärjestelmässä

OpenAIRE

Jutila, Tiina

2013-01-01

Opinnäytetyössä tutkittiin responsiivisen web-kehityksen toteuttamista WordPress-julkaisujärjes¬-telmässä. Tavoitteena oli toteuttaa yrityksen käyttöön WordPress-teema, joka sisälsi tarvittavat ominaisuudet mukautuvien verkkosivujen toteuttamiseen. Teeman tarkoituksena oli toimia pohjana, jonka päälle verkkosivun rakentaminen olisi mahdollisimman vaivatonta. Työssä käsiteltiin mobiililaitteiden asettamia haasteita web-kehityksessä, sekä tekniikoita niiden rat-kaisemiseen. Päätelaitteiden ...

Short-term variability of dwarf nova SS Cyg during outbursts

International Nuclear Information System (INIS)

Voloshina, I; Metlov, V; Rovithis-Livaniou, H

2009-01-01

Here we report the results of CCD observations of classical dwarf nova SS Cyg carried out with the two 60-cm telescopes in Crimea during the last years. These observations cover a few outbursts in 2006, 2007 and 2008. Power spectrum analysis of our CCD data clearly shows the existence of rapid periodic oscillations in the light curve of SS Cyg at the stage of decline after maximum. CCD observations of SS Cyg in autumn 2006 outburst revealed oscillations with the two periods 10 s and 76 s, in November 2007 - with 41 s period and in January 2008 with 98 s. We interpret detected variations as quasi-periodic oscillations.

Short-term variability of dwarf nova SS Cyg during outbursts

Energy Technology Data Exchange (ETDEWEB)

Voloshina, I; Metlov, V; Rovithis-Livaniou, H, E-mail: vib@sai.msu.r [Section of Astrophysics, Astronomy and Mechanics, Department of Physics, Athens University, Zagrafos 15784, Athens (Greece)

2009-06-01

Here we report the results of CCD observations of classical dwarf nova SS Cyg carried out with the two 60-cm telescopes in Crimea during the last years. These observations cover a few outbursts in 2006, 2007 and 2008. Power spectrum analysis of our CCD data clearly shows the existence of rapid periodic oscillations in the light curve of SS Cyg at the stage of decline after maximum. CCD observations of SS Cyg in autumn 2006 outburst revealed oscillations with the two periods 10 s and 76 s, in November 2007 - with 41 s period and in January 2008 with 98 s. We interpret detected variations as quasi-periodic oscillations.

Asymmetric PCR for good quality ssDNA generation towards DNA aptamer production

Directory of Open Access Journals (Sweden)

Junji Tominaga4

2012-04-01

Full Text Available Aptamers are ssDNA or RNA that binds to wide variety of target molecules with high affinity and specificity producedby systematic evolution of ligands by exponential enrichment (SELEX. Compared to RNA aptamer, DNA aptamer is muchmore stable, favourable to be used in many applications. The most critical step in DNA SELEX experiment is the conversion ofdsDNA to ssDNA. The purpose of this study was to develop an economic and efficient approach of generating ssDNA byusing asymmetric PCR. Our results showed that primer ratio (sense primer:antisense primer of 20:1 and sense primer amountof 10 to 100 pmol, up to 20 PCR cycles using 20 ng of initial template, in combination with polyacrylamide gel electrophoresis,were the optimal conditions for generating good quality and quantity of ssDNA. The generation of ssDNA via this approachcan greatly enhance the success rate of DNA aptamer generation.

ReSS: Resource Selection Service for National and Campus Grid Infrastructure

International Nuclear Information System (INIS)

Mhashilkar, Parag; Garzoglio, Gabriele; Levshina, Tanya; Timm, Steve

2010-01-01

The Open Science Grid (OSG) offers access to around hundred Compute elements (CE) and storage elements (SE) via standard Grid interfaces. The Resource Selection Service (ReSS) is a push-based workload management system that is integrated with the OSG information systems and resources. ReSS integrates standard Grid tools such as Condor, as a brokering service and the gLite CEMon, for gathering and publishing resource information in GLUE Schema format. ReSS is used in OSG by Virtual Organizations (VO) such as Dark Energy Survey (DES), DZero and Engagement VO. ReSS is also used as a Resource Selection Service for Campus Grids, such as FermiGrid. VOs use ReSS to automate the resource selection in their workload management system to run jobs over the grid. In the past year, the system has been enhanced to enable publication and selection of storage resources and of any special software or software libraries (like MPI libraries) installed at computing resources. In this paper, we discuss the Resource Selection Service, its typical usage on the two scales of a National Cyber Infrastructure Grid, such as OSG, and of a campus Grid, such as FermiGrid.

ReSS: Resource Selection Service for National and Campus Grid Infrastructure

Energy Technology Data Exchange (ETDEWEB)

Mhashilkar, Parag; Garzoglio, Gabriele; Levshina, Tanya; Timm, Steve, E-mail: parag@fnal.go, E-mail: garzogli@fnal.go, E-mail: tlevshin@fnal.go, E-mail: timm@fnal.go [Fermi National Accelerator Laboratory, P O Box 500, Batavia, IL - 60510 (United States)

2010-04-01

The Open Science Grid (OSG) offers access to around hundred Compute elements (CE) and storage elements (SE) via standard Grid interfaces. The Resource Selection Service (ReSS) is a push-based workload management system that is integrated with the OSG information systems and resources. ReSS integrates standard Grid tools such as Condor, as a brokering service and the gLite CEMon, for gathering and publishing resource information in GLUE Schema format. ReSS is used in OSG by Virtual Organizations (VO) such as Dark Energy Survey (DES), DZero and Engagement VO. ReSS is also used as a Resource Selection Service for Campus Grids, such as FermiGrid. VOs use ReSS to automate the resource selection in their workload management system to run jobs over the grid. In the past year, the system has been enhanced to enable publication and selection of storage resources and of any special software or software libraries (like MPI libraries) installed at computing resources. In this paper, we discuss the Resource Selection Service, its typical usage on the two scales of a National Cyber Infrastructure Grid, such as OSG, and of a campus Grid, such as FermiGrid.

ReSS: Resource Selection Service for National and Campus Grid Infrastructure

International Nuclear Information System (INIS)

Mhashilkar, Parag; Garzoglio, Gabriele; Levshina, Tanya; Timm, Steve

2009-01-01

The Open Science Grid (OSG) offers access to around hundred Compute elements (CE) and storage elements (SE) via standard Grid interfaces. The Resource Selection Service (ReSS) is a push-based workload management system that is integrated with the OSG information systems and resources. ReSS integrates standard Grid tools such as Condor, as a brokering service and the gLite CEMon, for gathering and publishing resource information in GLUE Schema format. ReSS is used in OSG by Virtual Organizations (VO) such as Dark Energy Survey (DES), DZero and Engagement VO. ReSS is also used as a Resource Selection Service for Campus Grids, such as FermiGrid. VOs use ReSS to automate the resource selection in their workload management system to run jobs over the grid. In the past year, the system has been enhanced to enable publication and selection of storage resources and of any special software or software libraries (like MPI libraries) installed at computing resources. In this paper, we discuss the Resource Selection Service, its typical usage on the two scales of a National Cyber Infrastructure Grid, such as OSG, and of a campus Grid, such as FermiGrid.

Differential Role of the T6SS in Acinetobacter baumannii Virulence

Science.gov (United States)

Foucault-Grunenwald, Marie-Laure; Borges, Vitor; Charpentier, Xavier; Limansky, Adriana S.; Gomes, João Paulo; Viale, Alejandro M.; Salcedo, Suzana P.

2015-01-01

Gram-negative bacteria, such as Acinetobacter baumannii, are an increasing burden in hospitals worldwide with an alarming spread of multi-drug resistant (MDR) strains. Herein, we compared a type strain (ATCC17978), a non-clinical isolate (DSM30011) and MDR strains of A. baumannii implicated in hospital outbreaks (Ab242, Ab244 and Ab825), revealing distinct patterns of type VI secretion system (T6SS) functionality. The T6SS genomic locus is present and was actively transcribed in all of the above strains. However, only the A. baumannii DSM30011 strain was capable of killing Escherichia coli in a T6SS-dependent manner, unlike the clinical isolates, which failed to display an active T6SS in vitro. In addition, DSM30011 was able to outcompete ATCC17978 as well as Pseudomonas aeruginosa and Klebsiella pneumoniae, bacterial pathogens relevant in mixed nosocomial infections. Finally, we found that the T6SS of DSM30011 is required for host colonization of the model organism Galleria mellonella suggesting that this system could play an important role in A. baumannii virulence in a strain-specific manner. PMID:26401654

Differential Role of the T6SS in Acinetobacter baumannii Virulence.

Directory of Open Access Journals (Sweden)

Guillermo D Repizo

Full Text Available Gram-negative bacteria, such as Acinetobacter baumannii, are an increasing burden in hospitals worldwide with an alarming spread of multi-drug resistant (MDR strains. Herein, we compared a type strain (ATCC17978, a non-clinical isolate (DSM30011 and MDR strains of A. baumannii implicated in hospital outbreaks (Ab242, Ab244 and Ab825, revealing distinct patterns of type VI secretion system (T6SS functionality. The T6SS genomic locus is present and was actively transcribed in all of the above strains. However, only the A. baumannii DSM30011 strain was capable of killing Escherichia coli in a T6SS-dependent manner, unlike the clinical isolates, which failed to display an active T6SS in vitro. In addition, DSM30011 was able to outcompete ATCC17978 as well as Pseudomonas aeruginosa and Klebsiella pneumoniae, bacterial pathogens relevant in mixed nosocomial infections. Finally, we found that the T6SS of DSM30011 is required for host colonization of the model organism Galleria mellonella suggesting that this system could play an important role in A. baumannii virulence in a strain-specific manner.

[Study and development of a new automatic suction system (SS) of intratracheal sputum in order to improve QOL of many patients with tracheostomy mechanical ventilation (TMV) and their family members by this new autonomic SS].

Science.gov (United States)

Hokezu, Youichi; Yamamoto, Makoto; Tokunaga, Syuichi; Niikura, Makoto; Nagamatsu, Keiji; Kira, Jun-ichi; Fukunaga, Toshihide; Shima, Kouji; Kikuchi, Seiji; Kimura, Itaru; Kondo, Kiyohiko; Mori, Teruhiko; Goto, Katumasa; Takigami, Shigeru; Shioya, Keiichi; Uehara, Minako

2009-11-01

In Japan, many patients equipped with TMV are under medical treatment at home after 1990. These patients can't put out sputa in trachea, so that these patient's family members must suck these patient's intratracheal sputa all days. Mr Yamamoto and Mr Tokunaga, main researchers of this study, began the study on the automatic SS of itratracheal sputa from 1999. In first stage, They developed the intermittent SS in detaining the suction tube within tracheal cannula, monitering the intratracheal pressure, but this system takes the ventilation away from the patient. Hypoventilation caused by this SS may cause the serious accident in patient. Therefore, we remodel the SS from intermittent SS to rollerpomp-type SS continuing to suck the itratracheal sputa with low volume from 2004, and thereafter we made up the SS of piston pomp type-SS finally at 2007. We developed the tracheal cannula with double suction holes of inner and lower hole in the lower part of its cannula together with the suction machine. We think that the practical use of this automatic SS will bring these patients with TMV and their family members great benefits. We desire that the practical use of this SS will be realized as soon as possible.

Is PiSS Alpha-1 Antitrypsin Deficiency Associated with Disease?

Directory of Open Access Journals (Sweden)

Dawn McGee

2010-01-01

Full Text Available Background. Alpha-1 antitrypsin deficiency (AAT is an inherited condition that predisposes to lung and/or liver disease. Objective. The current study examined the clinical features of the PiSS genotype. Methods. Nineteen study participants (PiSS and 29 matched control participants (PiMM were telephone interviewed using a standardized questionnaire. Demographic features, cigarette smoking, vocation, medication history, and clinical diagnoses were compared. Statistical analysis was performed. Finally, a comprehensive literature review was performed by two investigators. Results. 12/19 (63.2% study participants reported the presence of lung and/or liver disease compared to 12/29 (41.4% control participants. There trended toward having a higher frequency of medication allergies in the study population (42.11% versus 20.69%. Conclusions. The PiSS genotype was associated with a similar incidence of obstructive lung disease to controls. Selective bias intrinsic in testing for AAT deficiency and the rarity of the PiSS genotype will make future study of this association dependent on population-based tests.

MUFOLD-SS: New deep inception-inside-inception networks for protein secondary structure prediction.

Science.gov (United States)

Fang, Chao; Shang, Yi; Xu, Dong

2018-05-01

Protein secondary structure prediction can provide important information for protein 3D structure prediction and protein functions. Deep learning offers a new opportunity to significantly improve prediction accuracy. In this article, a new deep neural network architecture, named the Deep inception-inside-inception (Deep3I) network, is proposed for protein secondary structure prediction and implemented as a software tool MUFOLD-SS. The input to MUFOLD-SS is a carefully designed feature matrix corresponding to the primary amino acid sequence of a protein, which consists of a rich set of information derived from individual amino acid, as well as the context of the protein sequence. Specifically, the feature matrix is a composition of physio-chemical properties of amino acids, PSI-BLAST profile, and HHBlits profile. MUFOLD-SS is composed of a sequence of nested inception modules and maps the input matrix to either eight states or three states of secondary structures. The architecture of MUFOLD-SS enables effective processing of local and global interactions between amino acids in making accurate prediction. In extensive experiments on multiple datasets, MUFOLD-SS outperformed the best existing methods and other deep neural networks significantly. MUFold-SS can be downloaded from http://dslsrv8.cs.missouri.edu/~cf797/MUFoldSS/download.html. © 2018 Wiley Periodicals, Inc.

Polymorphism and solvates of 3,3'-dihydroxy-ss,ss-carotene-4,4'-dione: Screening and their thermodynamics

Energy Technology Data Exchange (ETDEWEB)

Guo, J.; Ulrich, J. [Martin Luther University Halle-Wittenberg, Center for Engineering Sciences/ TVT, 06099, Halle Saale (Germany)

2010-03-15

3,3'-dihydroxy-ss,ss-carotene-4,4'-dione (DCD) is a carotenoid used for the pink coloration in animal and fish foods. Two nonsolvated and two solvated forms of this compound have been discovered and characterized using different analytical techniques. The thermodynamic stability of the relevant polymorphs is revealed. The transformation rate depends strongly on the selection of solvent medium. Moreover, different chemical stability refers to the shelf life is studied correlating to the different polymorphs. The results show the possibilities to improve the pigmentation efficiency and chemical stability by a changing in the crystal polymorphs. (copyright 2010 WILEY-VCH Verlag GmbH and Co. KGaA, Weinheim) (orig.)

Efficient Asymmetric Synthesis of S,S-2-methylsulfanyl-2-methylsulfinyl-1-indanone

OpenAIRE

Derisvaldo Rosa Paiva; Roberto da Silva Gomes

2013-01-01

Diastereoselective synthesis of SS-2-methylsulfanyl-2-methylsulfinyl-1-indanol by reduction of SS-2-methylsulfanyl-2-methylsulfinyl-1-indanone optically enriched demonstrating to be highly efficiency using the sulfanyl group as asymmetric induction control agent during an addition reaction to carbonyl group.The 2-methylsulfinyl-1-indanone was obtained for the first time in one unique step without further oxidation steps. The synthesis of SR, SS of 2-methylsulphinyl-1-indanone optically enrich...

32 CFR Appendix E to Part 246 - Stars and Stripes (S&S) Board of Directors

Science.gov (United States)

2010-07-01

... of directors shall monitor planning and execution of the S&S business activities. 2. The S&S board of... DEFENSE (CONTINUED) MISCELLANEOUS STARS AND STRIPES (S&S) NEWSPAPER AND BUSINESS OPERATIONS Pt. 246, App. E Appendix E to Part 246—Stars and Stripes (S&S) Board of Directors A. Organization and Management...

History of the Balkan Stomatological Society (BaSS

Directory of Open Access Journals (Sweden)

Todorović Ljubomir

2014-11-01

Full Text Available Some of the main activities of the Balkan Stomatological Society (BaSS over a rich 19-year history are presented. These activities have been aimed at improving oral health care provided by the dentists throughout the Balkans, and to establish ties of friendship and collaboration between researchers and clinicians in this region, creating a foundation for mutual understanding and peace. To accomplish these goals, the BaSS annually organizes congresses and publishes a scientific journal, beside many other activities, such as public oral health promotion, bringing into accordance study programmes and curricula, supporting student exchange programmes, etc.

Swelling and microstructure of neutrons irradiated 316 Ti SS

International Nuclear Information System (INIS)

Seran, J.L.; Le Naour, L.; Grosjean, P.; Hugon, M.P.; Carteret, Y.; Maillard, A.

1984-06-01

The analysis of the behaviour of fuel pins irradiated in the same RAPSODIE subassembly, shows that titanium has a marked beneficial effect on the swelling resistance of CW 316 SS in a large range of temperature. This effect is particularly visible at high temperature since CW 316 Ti SS does not swell above 550 0 C up to a dose of 100 French dpa. The results obtained on samples irradiated in a RAPSODIE experimental rig give us confirmation of the good behaviour of CW 316 Ti SS which swells less and at smaller temperature than the other steels of the 316 series such as SA 316 Ti or aged SA 316 Ti. The swelling differences between some of these materials can be associated to different microstructures which are also very different from the ones obtained on the irradiated steels aged in the same time and temperature conditions

Architecture and ssDNA interaction of the Timeless-Tipin-RPA complex.

Science.gov (United States)

Witosch, Justine; Wolf, Eva; Mizuno, Naoko

2014-11-10

The Timeless-Tipin (Tim-Tipin) complex, also referred to as the fork protection complex, is involved in coordination of DNA replication. Tim-Tipin is suggested to be recruited to replication forks via Replication Protein A (RPA) but details of the interaction are unknown. Here, using cryo-EM and biochemical methods, we characterized complex formation of Tim-Tipin, RPA and single-stranded DNA (ssDNA). Tim-Tipin and RPA form a 258 kDa complex with a 1:1:1 stoichiometry. The cryo-EM 3D reconstruction revealed a globular architecture of the Tim-Tipin-RPA complex with a ring-like and a U-shaped domain covered by a RPA lid. Interestingly, RPA in the complex adopts a horse shoe-like shape resembling its conformation in the presence of long ssDNA (>30 nucleotides). Furthermore, the recruitment of the Tim-Tipin-RPA complex to ssDNA is modulated by the RPA conformation and requires RPA to be in the more compact 30 nt ssDNA binding mode. The dynamic formation and disruption of the Tim-Tipin-RPA-ssDNA complex implicates the RPA-based recruitment of Tim-Tipin to the replication fork. © The Author(s) 2014. Published by Oxford University Press on behalf of Nucleic Acids Research.

An extract of the medicinal mushroom Agaricus blazei Murill can protect against allergy

Directory of Open Access Journals (Sweden)

Hetland Geir

2009-05-01

Full Text Available Abstract Background Agaricus blazei Murill (AbM is an edible Brazilian mushroom that has been used in traditional medicine for a range of diseases. It has been shown to have anti-infection and anti-tumor properties in the mouse, which are due to induction of Th1 responses. On the other hand, IgE-mediated allergy is induced by a Th2 response. Objective Since according to the Th1/Th2 paradigm an increased Th1 response may promote a reduced Th2 response, the aim was to examine whether AbM had anti-allergy effects. Methods A mouse model for allergy was employed, in which the mice were immunized s.c. with the model allergen ovalbumin (OVA. Additionally, the animals were given a mushroom extract, AndoSan™, mainly (82% containing AbM, but also Hericium erinaceum (15% and Grifola frondosa (3%, or PBS p.o. either a day before or 19 days after the immunization. The mice were sacrificed on day 26, and anti-OVA IgE (Th2 response and IgG2a (Th1 response antibodies were examined in serum and Th1, Th2 and Treg cytokines in spleen cells cultures. Results It was found that the AndoSan™ extract both when given either before or after OVA immunization reduced the levels of anti-OVA IgE, but not IgG2a, in the mice. There was a tendency to reduced Th2 relative to Th1 cytokine levels in the AndoSan™ groups. Conclusion This particular AbM extract may both prevent allergy development and be used as a therapeutical substance against established allergy.

RPA homologs and ssDNA processing during meiotic recombination.

Science.gov (United States)

Ribeiro, Jonathan; Abby, Emilie; Livera, Gabriel; Martini, Emmanuelle

2016-06-01

Meiotic homologous recombination is a specialized process that involves homologous chromosome pairing and strand exchange to guarantee proper chromosome segregation and genetic diversity. The formation and repair of DNA double-strand breaks (DSBs) during meiotic recombination differs from those during mitotic recombination in that the homologous chromosome rather than the sister chromatid is the preferred repair template. The processing of single-stranded DNA (ssDNA) formed on intermediate recombination structures is central to driving the specific outcomes of DSB repair during meiosis. Replication protein A (RPA) is the main ssDNA-binding protein complex involved in DNA metabolism. However, the existence of RPA orthologs in plants and the recent discovery of meiosis specific with OB domains (MEIOB), a widely conserved meiosis-specific RPA1 paralog, strongly suggest that multiple RPA complexes evolved and specialized to subdivide their roles during DNA metabolism. Here we review ssDNA formation and maturation during mitotic and meiotic recombination underlying the meiotic specific features. We describe and discuss the existence and properties of MEIOB and multiple RPA subunits in plants and highlight how they can provide meiosis-specific fates to ssDNA processing during homologous recombination. Understanding the functions of these RPA homologs and how they interact with the canonical RPA subunits is of major interest in the fields of meiosis and DNA repair.

Validation of the Stroke Specific Quality of Life Scale (SS-QOL): test of reliability and validity of the Danish version (SS-QOL-DK).

Science.gov (United States)

Muus, Ingrid; Williams, Linda S; Ringsberg, Karin C

2007-07-01

To test the reliability and validity of the Danish version of the Stroke Specific Quality of Life Scale version 2.0 (SS-QOL-DK), an instrument for evaluation of health-related quality of life. A correlational study. A stroke unit that provides acute care and rehabilitation for stroke patients in Frederiksborg County, Denmark. One hundred and fifty-two stroke survivors participated; 24 of these performed test-retest. Questionnaires were sent out and returned by mail. A subsequent telephone interview assessed functional level and missing items. Test-retest was measured using Spearman's r, internal consistency was estimated using Cronbach's alpha, and evaluation of floor and ceiling values in proportion of minimum and maximum scores. Construct validity was assessed by comparing patients' scores on the SS-QOL-DK with those obtained by other test methods: Beck's Depression Index, the General Health Survey Short Form 36 (SF-36), the Barthel Index and the National Institutes of Health Stroke Scale, evaluating shared variance using coefficient of determination, r2. Comparing groups with known scores assessed known-group validity. Convergent and discriminant validity were assessed. Test-retest of SS-QOL-DK showed excellent stability, Spearman's r = 0.65-0.99. Internal consistency for all domains showed Cronbach's alpha = 0.81-0.94. Missing items rate was 1.0%. Most SS-QOL-DK domains showed moderately shared variance with similar domains of other test methods, r2 = 0.03-0.62. Groups with known differences showed statistically significant difference in scores. Item-to-scale correlation coefficients of 0.37-0.88 supported convergent validity. SS-QOL-DK is a reliable and valid instrument for measuring self-reported health-related quality of life on group level among people with mild to moderate stroke.

THE Be STAR SPECTRA (BeSS) DATABASE

International Nuclear Information System (INIS)

Neiner, C.; De Batz, B.; Cochard, F.; Floquet, M.; Mekkas, A.; Desnoux, V.

2011-01-01

Be stars vary on many timescales, from hours to decades. A long time base of observations to analyze certain phenomena in these stars is therefore necessary. Collecting all existing and future Be star spectra into one database has thus emerged as an important tool for the Be star community. Moreover, for statistical studies, it is useful to have centralized information on all known Be stars via an up-to-date catalog. These two goals are what the Be Star Spectra (BeSS, http://basebe.obspm.fr) database proposes to achieve. The database contains an as-complete-as-possible catalog of known Be stars with stellar parameters, as well as spectra of Be stars from all origins (any wavelength, any epoch, any resolution, etc.). It currently contains over 54,000 spectra of more than 600 different Be stars among the ∼2000 Be stars in the catalog. A user can access and query this database to retrieve information on Be stars or spectra. Registered members can also upload spectra to enrich the database. Spectra obtained by professional as well as amateur astronomers are individually validated in terms of format and science before being included in BeSS. In this paper, we present the database itself as well as examples of the use of BeSS data in terms of statistics and the study of individual stars.

Is PiSS Alpha-1 Antitrypsin Deficiency Associated with Disease?

OpenAIRE

McGee, Dawn; Schwarz, Laura; McClure, Rebecca; Peterka, Lauren; Rouhani, Farshid; Brantly, Mark; Strange, Charlie

2010-01-01

Background. Alpha-1 antitrypsin deficiency (AAT) is an inherited condition that predisposes to lung and/or liver disease. Objective. The current study examined the clinical features of the PiSS genotype. Methods. Nineteen study participants (PiSS) and 29 matched control participants (PiMM) were telephone interviewed using a standardized questionnaire. Demographic features, cigarette smoking, vocation, medication history, and clinical diagnoses were compared. Statistical analysis was perform...

How to Distinguish Patients with pSS among Individuals with Dryness without Invasive Diagnostic Studies

Directory of Open Access Journals (Sweden)

Agata Sebastian

2018-01-01

Full Text Available In the course of pSS, inflammatory cell infiltration consists mainly of lymphocytes infiltrating exocrine glands, which leads to their impaired function. The characteristic feature is generalized dryness. The aim of this study was to attempt to answer the question whether it is possible to distinguish between patients with pSS and individuals with dryness caused by other pathologies without applying invasive studies. The study included 68 patients with pSS and 43 healthy controls with dryness. FS ≥ 1 was observed in 90% of patients with pSS (with or without dryness, and only in 23% of the control group (only with xerostomia. In the pSS group, anaemia (p=0.0085, lymphocytopenia (p=0.0006, elevated ERS (p=0.001, higher RF titer, and ANA antibodies were noted. Configuration of anti-SSA + SSB + Ro52 antibodies was characteristic for the pSS group. Considering the clinical symptoms, statistically significant differences were noted between pSS patients and the control group in frequency (p=0.02 and severity (p=0.042 of fatigue, lymphadenopathy, major salivary gland involvement, and photosensitivity to UV light. In conclusion, invasive methods are pivotal in pSS diagnosis in this salivary gland biopsy. Chronic fatigue syndrome is more common in pSS patients and can be subjective distinguishing factor in the group of people with dryness.

Osakkeenomistajan kunnossapitovastuu ja muutostyöoikeus asunto-osakeyhtiössä

OpenAIRE

Kujanpää, Vesa

2010-01-01

Opinnäytetyössä oli tavoitteena selvittää 01.07.2010 muuttuneen asunto-osakeyhtiölain mukaista osakkeenomistajan kunnossapitovastuuta sekä oikeutta tehdä muutostöitä omistamassaan osakehuoneistossa. Lisäksi työssä käsiteltiin uuden lain mukaista korjaustyöhön liittyvää ilmoitus- ja lupamenettelyä. Työssä tutkittiin lain soveltamista käytäntöön ja pohdittiin lainmukaisen toiminnan tuomia lisäkustannuksia ja niiden oikeaa tasoa. Lisäksi työn tavoitteena oli auttaa isännöitsijöitä ymmärtämään, ...

Digipelaamisen hyödyt mielenterveystyössä

OpenAIRE

Simula, Kristiina

2017-01-01

Mielenterveyshäiriöitä ilmenee joka viidennellä suomalaisella. Avohoitopainotteiseen mielenterveystyöhön siirryttäessä tarvitaan uusia näkökulmia asiakastyöhön. DIgipelien suosion myötä on tärkeää miettiä niiden käyttömahdollisuuksia mielenterveystyössä. Jotta uudet menetelmät voidaan ottaa käyttöön, tarvitaan digipelien hyödyistä tutkimustietoa. Opinnäytetyön tarkoituksena on kuvata mitä tutkimustietoa on saatavilla digipelaamisen hyödyistä mielenterveystyössä. Opinnäytetyön tavoitteena ...

Previously unknown and highly divergent ssDNA viruses populate the oceans.

Science.gov (United States)

Labonté, Jessica M; Suttle, Curtis A

2013-11-01

Single-stranded DNA (ssDNA) viruses are economically important pathogens of plants and animals, and are widespread in oceans; yet, the diversity and evolutionary relationships among marine ssDNA viruses remain largely unknown. Here we present the results from a metagenomic study of composite samples from temperate (Saanich Inlet, 11 samples; Strait of Georgia, 85 samples) and subtropical (46 samples, Gulf of Mexico) seawater. Most sequences (84%) had no evident similarity to sequenced viruses. In total, 608 putative complete genomes of ssDNA viruses were assembled, almost doubling the number of ssDNA viral genomes in databases. These comprised 129 genetically distinct groups, each represented by at least one complete genome that had no recognizable similarity to each other or to other virus sequences. Given that the seven recognized families of ssDNA viruses have considerable sequence homology within them, this suggests that many of these genetic groups may represent new viral families. Moreover, nearly 70% of the sequences were similar to one of these genomes, indicating that most of the sequences could be assigned to a genetically distinct group. Most sequences fell within 11 well-defined gene groups, each sharing a common gene. Some of these encoded putative replication and coat proteins that had similarity to sequences from viruses infecting eukaryotes, suggesting that these were likely from viruses infecting eukaryotic phytoplankton and zooplankton.

Chitin-induced T6SS in Vibrio cholerae is dependent on ChiS activation.

Science.gov (United States)

Chourashi, Rhishita; Das, Suman; Dhar, Debarpan; Okamoto, Keinosuke; Mukhopadhyay, Asish K; Chatterjee, Nabendu Sekhar

2018-05-01

Vibrio cholerae regularly colonizes the chitinous exoskeleton of crustacean shells in the aquatic region. The type 6 secretion system (T6SS) in V. cholerae is an interbacterial killing device. This system is thought to provide a competitive advantage to V. cholerae in a polymicrobial community of the aquatic region under nutrient-poor conditions. V. cholerae chitin sensing is known to be initiated by the activation of a two-component sensor histidine kinase ChiS in the presence of GlcNAc2 (N,N'-diacetylchitobiose) residues generated by the action of chitinases on chitin. It is known that T6SS in V. cholerae is generally induced by chitin. However, the effect of ChiS activation on T6SS is unknown. Here, we found that ChiS inactivation resulted in impaired bacterial killing and reduced expression of T6SS genes. Active ChiS positively affected T6SS-mediated natural transformation in V. cholerae. ChiS depletion or inactivation also resulted in reduced colonization on insoluble chitin surfaces. Therefore, we have shown that V. cholerae colonization on chitinous surfaces activates ChiS, which promotes T6SS-dependent bacterial killing and horizontal gene transfer. We also highlight the importance of chitinases in T6SS upregulation.

The Symbiotic System SS73 17 seen with Suzaku

Science.gov (United States)

Smith, Randall K.; Mushotzky, Richard; Kallman, Tim; Tueller, Jack; Mukai, Koji; Markwardt, Craig

2007-01-01

We observed with Suzaku the symbiotic star SS73 17, motivated by the discovery by the INTEGRAL satellite and the Swift BAT survey that it emits hard X-rays. Our observations showed a highly-absorbed X-ray spectrum with NH > loz3 emp2, equivalent to Av > 26, although the source has B magnitude 11.3 and is also bright in UV. The source also shows strong, narrow iron lines including fluorescent Fe K as well as Fe xxv and Fe XXVI. The X-ray spectrum can be fit with a thermal model including an absorption component that partially covers the source. Most of the equivalent width of the iron fluorescent line in this model can be explained as a combination of reprocessing in a dense absorber plus reflection off a white dwarf surface, but it is likely that the continuum is partially seen in reflection as well. Unlike other symbiotic systems that show hard X-ray emission (CH Cyg, RT Cru, T CrB, GX1+4), SS73 17 is not known to have shown nova-like optical variability, X-ray flashes, or pulsations, and has always shown faint soft X-ray emission. As a result, although it is likely a white dwarf, the nature of the compact object in SS73 17 is still uncertain. SS73 17 is probably an extreme example of the recently discovered and relatively small class of hard X-ray emitting symbiotic systems.

TIMES-SS - A mechanistic evaluation of an external validation study using reaction chemistry principles

DEFF Research Database (Denmark)

Roberts, David W.; Patlewicz, Grace; Dimitrov, Sabcho D.

2007-01-01

The TImes MEtabolism Simulator platform used for predicting skin sensitization (TIMES-SS) is a hybrid expert system that was developed at Bourgas University using funding and data from a consortium comprised of industry and regulators. TIMES-SS encodes structure-toxicity and structure...... chemicals in the murine local lymph node assay (LLNA) and then compared with predictions made by TIMES-SS. The results were promising with an overall good concordance (83%) between experimental and predicted values. The LLNA results were evaluated with respect to reaction chemistry principles...... for sensitization. Additional testing on a further four chemicals was carried out to explore some of the specific reaction chemistry findings in more detail. Improvements for TIMES-SS, where appropriate, were put forward together with proposals for further research work. TIMES-SS is a promising tool to aid...

SS433: the second Wolf-Rayet X-ray binary ?

OpenAIRE

Fuchs, Yael; Koch-Miramond, Lydie; Abraham, Peter

2002-01-01

We present mid-infrared spectrophotometric observations of SS433 with ISOPHOT. The HeI+HeII lines in both spectra of SS433 and of the Wolf-Rayet star WR147, a wind-colliding WN8+BO5 binary system, closely match. The 2.5-12 micron continuum radiation is due to an expanding wind free-free emission in an intermediate case between optically thick and optically thin regimes. The inferred mass loss rate evaluation gives ~10^{-4} Msun/yr. Our results are consistent with a Wolf-Rayet-like companion t...

Design basis ground motion (Ss) required on new regulatory guide

International Nuclear Information System (INIS)

Kamae, Katsuhiro

2013-01-01

New regulatory guide is enforced on July 8. Here, it is introduced how the design basis ground motion (Ss) for seismic design of nuclear power reactor facilities was revised on the new guide. Ss is formulated as two types of earthquake ground motions, earthquake ground motions with site specific earthquake source and with no such specific source locations. The latter is going to be revised based on the recent observed near source ground motions. (author)

Nuorisopsykiatrisessa hoitotyössä jaksaminen, kun itsellä alaikäisiä lapsia

OpenAIRE

Kuuslehto, Marianna; Niemi, Mari

2014-01-01

Tämän opinnäytetyön tarkoituksena oli kuvata sairaanhoitajien jaksamista nuorisopsykiatrisessa hoitotyössä, kun heillä itsellään on alaikäisiä lapsia. Opinnäytetyön tavoitteena on saada tietoa, millaisena sairaanhoitajat kokevat työssäjaksamisen nuorisopsykiatrisessa hoitotyössä, kun itsellä on alaikäisiä lapsia. Tavoitteena on myös saada tietoa sairaanhoitajien työssäjaksamisen keinoista ja nuorisopsykiatrisen työn kuormittavuustekijöistä. Opinnäytetytön avulla saatavaa tietoa voidaan hyödyn...

Efficient Asymmetric Synthesis of S,S-2-methylsulfanyl-2-methylsulfinyl-1-indanone

Directory of Open Access Journals (Sweden)

Derisvaldo Rosa Paiva

2013-05-01

Full Text Available Diastereoselective synthesis of SS-2-methylsulfanyl-2-methylsulfinyl-1-indanol by reduction of SS-2-methylsulfanyl-2-methylsulfinyl-1-indanone optically enriched demonstrating to be highly efficiency using the sulfanyl group as asymmetric induction control agent during an addition reaction to carbonyl group.The 2-methylsulfinyl-1-indanone was obtained for the first time in one unique step without further oxidation steps. The synthesis of SR, SS of 2-methylsulphinyl-1-indanone optically enriched in good yield and good enantiomeric excess determined by nuclear magnetic resonance technique employing the Kagan reagent as chiral shift agent.

Synergistic Protective Effects of Mitochondrial Division Inhibitor 1 and Mitochondria-Targeted Small Peptide SS31 in Alzheimer's Disease.

Science.gov (United States)

Reddy, P Hemachandra; Manczak, Maria; Yin, XiangLing; Reddy, Arubala P

2018-01-01

The purpose of our study was to determine the synergistic protective effects of mitochondria-targeted antioxidant SS31 and mitochondria division inhibitor 1 (Mdivi1) in Alzheimer's disease (AD). Using biochemical methods, we assessed mitochondrial function by measuring the levels of hydrogen peroxide, lipid peroxidation, cytochrome c oxidase activity, mitochondrial ATP, and GTPase Drp1 enzymatic activity in mutant AβPP cells. Using biochemical methods, we also measured cell survival and apoptotic cell death. Amyloid-β (Aβ) levels were measured using sandwich ELISA, and using real-time quantitative RT-PCR, we assessed mtDNA (mtDNA) copy number in relation to nuclear DNA (nDNA) in all groups of cells. We found significantly reduced levels of Aβ40 and Aβ42 in mutant AβPP cells treated with SS31, Mdivi1, and SS31+Mdivi1, and the reduction of Aβ42 levels were much higher in SS31+Mdivi1 treated cells than individual treatments of SS31 and Mdivi1. The levels of mtDNA copy number and cell survival were significantly increased in SS31, Mdivi1, and SS31+Mdivi1 treated mutant AβPP cells; however, the increased levels of mtDNA copy number and cell survival were much higher in SS31+Mdivi1 treated cells than individual treatments of SS31 and Mdivi1. Mitochondrial dysfunction is significantly reduced in SS31, Mdivi1, and SS31+Mdivi1 treated mutant AβPP cells; however, the reduction is much higher in cells treated with both SS31+Mdvi1. Similarly, GTPase Drp1 activity is reduced in all treatments, but reduced much higher in SS31+Mdivi1 treated cells. These observations strongly suggest that combined treatment of SS31+Mdivi1 is effective than individual treatments of SS31 and Mdivi1. Therefore, we propose that combined treatment of SS31+Mdivi1 is a better therapeutic strategy for AD. Ours is the first study to investigate combined treatment of mitochondria-targeted antioxidant SS31 and mitochondrial division inhibitor 1 in AD neurons.

28 November 2013 - N. N. Kudryavtsev, Russian Rector of the Moscow Institute of Physics and Technology signing an Agreement and the Guest Book with CERN Director-General R. Heuer; visiting the ATLAS cavern with ATLAS Deputy Spokesperson B. Heinemann and visiting the LHC tunnel at Point 1 with AGH University of Science and Technology A. Erokhin. M. Savino, Physics Department, Joint Institute for Nuclear Research also present.

CERN Multimedia

Jean-Claude Gadmer

2013-01-01

28 November 2013 - N. N. Kudryavtsev, Russian Rector of the Moscow Institute of Physics and Technology signing an Agreement and the Guest Book with CERN Director-General R. Heuer; visiting the ATLAS cavern with ATLAS Deputy Spokesperson B. Heinemann and visiting the LHC tunnel at Point 1 with AGH University of Science and Technology A. Erokhin. M. Savino, Physics Department, Joint Institute for Nuclear Research also present.

Changes of plasma SS, SP contents in adult patients with primary hypothyroidism

International Nuclear Information System (INIS)

Zheng Xianghong; Song Changyi; Lei Yamei; Ning Ning; Chen Wei; Li Runming

2008-01-01

Objective: To investigate the possible mechanism of imparirment of central nervous system function in hypothyroid patients through determination of changes of plasma neuropeptides after thyroid hormone replacement therapy. Methods: Plasma somatostatin (SS) and substance P(SP) contents were measured with RIA in 45 patients with primary hypothyroidism both before and after thyroid hormone replacement therapy as well as in 38 controls. Results: Before treatment, the plasma contents of SP in the patients were significantly lower than those in the controls (P 0.05). However, the plasma contents of SS in the more advanced hypothyroid patients with FT 3 3 , FT 4 levels, the plasma SS, SP increased significantly (vs before treatment P<0.05, P<0.01). Conclusion: The decrease of plasma contents of SS and SP in patients with hypothyroidism might be related to the development of psycho-neurological symptoms in these patients and thyroid hormone replacement therapy was desirable. (authors)

CoordSS: An Ontology Framework for Heterogeneous Networks Experimentation

Directory of Open Access Journals (Sweden)

V. Nejkovic

2016-11-01

Full Text Available Experimenting with HetNets environments is of importance because of the role that such environments have in next-generation cellular networks. In this paper, the CoordSS ontology experimentation framework is proposed with an aim to support experimenting with HetNets environments on wireless networking testbeds. In the framework, domain and system ontologies are adopted for formal representation of the knowledge about the context of the problem. This paper outlines implementation details of ontologies in the CoordSS experimentation framework. The synergy between semantic and cognitive computing is introduced as the theoretical foundation of the paper.

VMD-SS: A graphical user interface plug-in to calculate the protein secondary structure in VMD program.

Science.gov (United States)

Yahyavi, Masoumeh; Falsafi-Zadeh, Sajad; Karimi, Zahra; Kalatarian, Giti; Galehdari, Hamid

2014-01-01

The investigation on the types of secondary structure (SS) of a protein is important. The evolution of secondary structures during molecular dynamics simulations is a useful parameter to analyze protein structures. Therefore, it is of interest to describe VMD-SS (a software program) for the identification of secondary structure elements and its trajectories during simulation for known structures available at the Protein Data Bank (PDB). The program helps to calculate (1) percentage SS, (2) SS occurrence in each residue, (3) percentage SS during simulation, and (4) percentage residues in all SS types during simulation. The VMD-SS plug-in was designed using TCL script and stride to calculate secondary structure features. The database is available for free at http://science.scu.ac.ir/HomePage.aspx?TabID=13755.

SS 383: A NEW S-TYPE YELLOW SYMBIOTIC STAR?

Energy Technology Data Exchange (ETDEWEB)

Baella, N. O.; Pereira, C. B. [Observatório Nacional, Rua José Cristino 77, CEP 20921-400, São Cristóvão, Rio de Janeiro (Brazil); Miranda, L. F. [Departamento de Física Aplicada, Facultad de Ciencias, Universidad de Vigo, E-36310 Vigo (Spain)

2013-11-01

Symbiotic stars are key objects in understanding the formation and evolution of interacting binary systems, and are probably the progenitors of Type Ia supernovae. However, the number of known symbiotic stars is much lower than predicted. We aim to search for new symbiotic stars, with particular emphasis on the S-type yellow symbiotic stars, in order to determine their total population, evolutionary timescales, and physical properties. The Two Micron All Sky Survey (2MASS) (J – H) versus (H – K {sub s}) color-color diagram has been previously used to identify new symbiotic star candidates and show that yellow symbiotics are located in a particular region of that diagram. Candidate symbiotic stars are selected on the basis of their locus in the 2MASS (J – H) versus (H – K {sub s}) diagram and the presence of Hα line emission in the Stephenson and Sanduleak Hα survey. This diagram separates S-type yellow symbiotic stars from the rest of the S-type symbiotic stars, allowing us to select candidate yellow symbiotics. To establish the true nature of the candidates, intermediate-resolution spectroscopy is obtained. We have identified the Hα emission line source SS 383 as an S-type yellow symbiotic candidate by its position in the 2MASS color-color diagram. The optical spectrum of SS 383 shows Balmer, He I, He II, and [O III] emission lines, in combination with TiO absorption bands that confirm its symbiotic nature. The derived electron density (≅10{sup 8-9} cm{sup –3}), He I emission line intensity ratios, and position in the [O III] λ5007/Hβ versus [O III] λ4363/Hγ diagram indicate that SS 383 is an S-type symbiotic star, with a probable spectral type of K7-M0 deduced for its cool component based on TiO indices. The spectral type and the position of SS 383 (corrected for reddening) in the 2MASS color-color diagram strongly suggest that SS 383 is an S-type yellow symbiotic. Our result points out that the 2MASS color-color diagram is a powerful tool in

SS 383: A NEW S-TYPE YELLOW SYMBIOTIC STAR?

International Nuclear Information System (INIS)

Baella, N. O.; Pereira, C. B.; Miranda, L. F.

2013-01-01

Symbiotic stars are key objects in understanding the formation and evolution of interacting binary systems, and are probably the progenitors of Type Ia supernovae. However, the number of known symbiotic stars is much lower than predicted. We aim to search for new symbiotic stars, with particular emphasis on the S-type yellow symbiotic stars, in order to determine their total population, evolutionary timescales, and physical properties. The Two Micron All Sky Survey (2MASS) (J – H) versus (H – K s ) color-color diagram has been previously used to identify new symbiotic star candidates and show that yellow symbiotics are located in a particular region of that diagram. Candidate symbiotic stars are selected on the basis of their locus in the 2MASS (J – H) versus (H – K s ) diagram and the presence of Hα line emission in the Stephenson and Sanduleak Hα survey. This diagram separates S-type yellow symbiotic stars from the rest of the S-type symbiotic stars, allowing us to select candidate yellow symbiotics. To establish the true nature of the candidates, intermediate-resolution spectroscopy is obtained. We have identified the Hα emission line source SS 383 as an S-type yellow symbiotic candidate by its position in the 2MASS color-color diagram. The optical spectrum of SS 383 shows Balmer, He I, He II, and [O III] emission lines, in combination with TiO absorption bands that confirm its symbiotic nature. The derived electron density (≅10 8-9 cm –3 ), He I emission line intensity ratios, and position in the [O III] λ5007/Hβ versus [O III] λ4363/Hγ diagram indicate that SS 383 is an S-type symbiotic star, with a probable spectral type of K7-M0 deduced for its cool component based on TiO indices. The spectral type and the position of SS 383 (corrected for reddening) in the 2MASS color-color diagram strongly suggest that SS 383 is an S-type yellow symbiotic. Our result points out that the 2MASS color-color diagram is a powerful tool in identifying new S

Immunization with the recombinant antigen Ss-IR induces protective immunity to infection with Strongyloides stercoralis in mice.

Science.gov (United States)

Abraham, David; Hess, Jessica A; Mejia, Rojelio; Nolan, Thomas J; Lok, James B; Lustigman, Sara; Nutman, Thomas B

2011-10-19

Human intestinal infections with the nematode Strongyloides stercoralis remain a significant problem worldwide and a vaccine would be a useful addition to the tools available to prevent and control this infection. The goal of this study was to test single antigens for their efficacy in a vaccine against S. stercoralis larvae in mice. Alum was used as the adjuvant in these studies and antigens selected for analysis were either recognized by protective human IgG (Ss-TMY-1, Ss-EAT-6, and Ss-LEC-5) or were known to be highly immunogenic in humans (Ss-NIE-1 and Ss-IR). Only mice immunized with the Ss-IR antigen demonstrated a significant decrease of approximately 80% in the survival of larval parasites in the challenge infection. Antibodies, recovered from mice with protective immunity to S. stercoralis after immunization with Ss-IR, were used to locate the antigen in the larvae. Confocal microscopy revealed that IgG from mice immunized with Ss-IR bound to the surface of the parasites and observations by electron microscopy indicated that IgG bound to granules in the glandular esophagus. Serum collected from mice immunized with Ss-IR passively transferred immunity to naïve mice. These studies demonstrate that Ss-IR, in combination with alum, induces high levels of protective immunity through an antibody dependent mechanism and may therefore be suitable for further development as a vaccine against human strongyloidiasis. Copyright © 2011 Elsevier Ltd. All rights reserved.

Rekrytointi Myyntityössä : vaikutus & kehittäminen

OpenAIRE

Koskinen, Severi

2017-01-01

Opinnäytetyön tavoitteena oli tutkia rekrytointia myyntityössä, sen vaikutusta ja miten kehittää rekrytointia. Tätä lähdettiin selvittämään käsittelemällä teoriaosuudessa rekrytoinnin vaikutuksia, vaikutuksia myyntityössä sekä rekrytointiprosessin suorittamista. Tutkimusosuudessa tätä pyrittiin selvittämään etsimällä onnistuneista rekrytoinneista yhteisiä tekijöitä sekä epäonnistuneille rekrytoinneille syitä. Näitä tunnistamalla pystytään kehittämään ennalta toimivia rekrytointeja sekä välttä...

Cytotoxic Vibrio T3SS1 Rewires Host Gene Expression to Subvert Cell Death Signaling and Activate Cell Survival Networks

Science.gov (United States)

De Nisco, Nicole J.; Kanchwala, Mohammed; Li, Peng; Fernandez, Jessie; Xing, Chao; Orth, Kim

2017-01-01

Bacterial effectors are potent manipulators of host signaling pathways. The marine bacterium Vibrio parahaemolyticus (V. para), delivers effectors into host cells through two type three secretion systems (T3SS). The ubiquitous T3SS1 is vital for V. para survival in the environment, whereas T3SS2 causes acute gastroenteritis in human hosts. Although the natural host is undefined, T3SS1 effectors attack highly conserved cellular processes and pathways to orchestrate non-apoptotic cell death. Much is known about how T3SS1 effectors function in isolation, but we wanted to understand how their concerted action globally affects host cell signaling. To assess the host response to T3SS1, we compared gene expression changes over time in primary fibroblasts infected with V. para that have a functional T3SS1 (T3SS1+) to those in cells infected with V. para lacking T3SS1 (T3SS1−). Overall, the host transcriptional response to both T3SS1+ and T3SS1− V. para was rapid, robust, and temporally dynamic. T3SS1 re-wired host gene expression by specifically altering the expression of 398 genes. Although T3SS1 effectors target host cells at the posttranslational level to cause cytotoxicity, network analysis indicated that V. para T3SS1 also precipitates a host transcriptional response that initially activates cell survival and represses cell death networks. The increased expression of several key pro-survival transcripts mediated by T3SS1 was dependent on a host signaling pathway that is silenced later in infection by the posttranslational action of T3SS1. Taken together, our analysis reveals a complex interplay between roles of T3SS1 as both a transcriptional and posttranslational manipulator of host cell signaling. PMID:28512145

Differential biotin labelling of the cell envelope proteins in lipopolysaccharidic diderm bacteria: Exploring the proteosurfaceome of Escherichia coli using sulfo-NHS-SS-biotin and sulfo-NHS-PEG4-bismannose-SS-biotin.

Science.gov (United States)

Monteiro, Ricardo; Chafsey, Ingrid; Leroy, Sabine; Chambon, Christophe; Hébraud, Michel; Livrelli, Valérie; Pizza, Mariagrazia; Pezzicoli, Alfredo; Desvaux, Mickaël

2018-06-15

Surface proteins are the major factor for the interaction between bacteria and its environment, playing an important role in infection, colonisation, virulence and adaptation. However, the study of surface proteins has proven difficult mainly due to their hydrophobicity and/or relatively low abundance compared with cytoplasmic proteins. To overcome these issues new proteomic strategies have been developed, such as cell-surface protein labelling using biotinylation reagents. Sulfo-NHS-SS-biotin is the most commonly used reagent to investigate the proteins expressed at the cell surface of various organisms but its use in lipopolysaccharidic diderm bacteria (archetypical Gram-negative bacteria) remains limited to a handful of species. While generally pass over in silence, some periplasmic proteins, but also some inner membrane lipoproteins, integral membrane proteins and cytoplasmic proteins (cytoproteins) are systematically identified following this approach. To limit cell lysis and diffusion of the sulfo-NHS-SS-biotin through the outer membrane, biotin labelling was tested over short incubation times and proved to be as efficient for 1 min at room temperature. To further limit labelling of protein located below the outer membrane, the use of high-molecular weight sulfo-NHS-PEG4-bismannose-SS-biotin appeared to recover differentially cell-envelope proteins compared to low-molecular weight sulfo-NHS-SS-biotin. Actually, the sulfo-NHS-SS-biotin recovers at a higher extent the proteins completely or partly exposed in the periplasm than sulfo-NHS-PEG4-bismannose-SS-biotin, namely periplasmic and integral membrane proteins as well as inner membrane and outer membrane lipoproteins. These results highlight that protein labelling using biotinylation reagents of different sizes provides a sophisticated and accurate way to differentially explore the cell envelope proteome of lipopolysaccharidic diderm bacteria. While generally pass over in silence, some periplasmic proteins

Study on the IFNL4 gene ss469415590 variant in Ukrainian population

Directory of Open Access Journals (Sweden)

Kucherenko A. M.

2014-09-01

Full Text Available Aim. To determine genotype and allele disribution for the IFNL4 gene ss469415590 and examine it for linkage with the IL28B gene rs12979860 in Ukrainian population. Methods. The studied group consisted of 100 unrelated donors of Eastern European origin representing the population of Ukraine. Genotyping for the IFNL4 gene ss469415590 was performed using the amplification-refractory mutation system PCR. Genotyping for the IL28B gene rs12979860 was performed by the PCR-based restriction fragment length polymorphism assay. Results. Genotype frequencies for both studied variants showed no significant deviation from those expected according to Hardy-Weinberg equilibrium. Allelic distribution for ss469415590 was: TT – 0.665, G – 0.335. Allelic frequencies of rs12979860 were: C – 0.655, T – 0.345. The results of likelihood ratio test indicated a linkage disequilibrium between the studied variants (p > 0.0001, the major alleles ss469415590 TT and rs12979860 C were in phase. The genetic structure of Ukrainian population in terms of two studied polymorphic variants is similar to the European population presented in the «1000 genomes» project. Conclusions. Considering a tight linkage revealed in Ukrainian population between the ss469415590 variant and rs12979860, a crucial genetic marker of chronic hepatitis C treatment efficiency, this polymorphism might be a promising target for further investigation as a pharmacogenetic marker.

A polysaccharide isolated from Agaricus blazei Murill (ABP-AW1) as a potential Th1 immunity-stimulating adjuvant.

Science.gov (United States)

Cui, Liran; Sun, Yongxu; Xu, Hao; Xu, Huiyu; Cong, Huan; Liu, Jicheng

2013-10-01

In the present study, a low molecular weight polysaccharide, ABP-AW1, isolated from Agaricus blazei Murill was assessed for its potential adjuvant activity. ABP-AW1 is considered to create a 'depot' of antigen at a subcutaneous injection site. ICR mice were immunized with 100 μg ovalbumin (OVA) alone or with 100 μg OVA formulated in 0.9% saline containing 200 μg aluminum (alum) or ABP-AW1 (50, 100 and 200 μg) on days 1 and 15. Two weeks after the secondary immunization, splenocyte proliferation, the expression of surface markers, cytokine production and the OVA-specific antibody levels in the serum were determined. The OVA/ABP-AW1 vaccine, in comparison with OVA alone, markedly increased the proliferation of splenic lymphocytes and elicited greater antigen-specific CD4 + T cell activation, as determined by splenic CD4 + CD69 + T cells and Th1 cytokine interferon (IFN)-γ release. The combination of ABP-AW1 and OVA also enhanced IgG2b antibody responses to OVA. In conclusion, these data indicated that ABP-AW1 significantly enhanced the humoral and cellular immune responses against OVA in the mice, suggesting that ABP-AW1 stimulated Th1-type immunity. We suggest that ABP-AW1 may serve as a new adjuvant.

Effect of Agaricus blazei Murrill extract on HT-29 human colon cancer cells in SCID mice in vivo.

Science.gov (United States)

Wu, Ming-Fang; Chen, Yung-Liang; Lee, Mei-Hui; Shih, Yung-Luen; Hsu, Yu-Ming; Tang, Ming-Chu; Lu, Hsu-Feng; Tang, Nou-Ying; Yang, Su-Tso; Chueh, Fu-Shin; Chung, Jing-Gung

2011-01-01

Agaricus blazei Murrill (ABM) popularly known as 'Cogumelo do Sol' in Brazil, or 'Himematsutake' in Japan, is a mushroom native to Brazil and widely cultivated in Japan for its medicinal uses and is now considered one of the most important edible and culinary-medicinal biotechnological species. This study is the first tumor growth model to evaluate the amelioratory effect of ABM extract using HT-29 human colon cancer cells in severe combined immunodeficiency (SCID) mice. Forty SCID mice were inoculated with HT-29 cells to induce tumor formation and were then divided into four groups. All the four groups (control, low, medium and high concentration treatment) of mice were separately orally administered 0 mg, 1.125 mg, 4.5 mg or 45 mg ABM extract daily. After six weeks of treatment, 8 out of the 40 mice had not survived including one mouse which scored +++ (tumor up to 15 mm diameter) and four mice which scored ++++ (tumor over 15 mm diameter) in the control group and three mice which scored ++++ on the low-dose ABM treatment. After high- or medium-dose treatment, all ten mice in each group survived. The oral administration of ABM does not prevent tumor growth, as shown by increased tumor mass, but compared with the control group, the tumor mass seems to grow more slowly depending on the ABM dose.

Analysis of the stress-inducible transcription factor SsNAC23 in sugarcane plants

Directory of Open Access Journals (Sweden)

Renata Fava Ditt

2011-08-01

Full Text Available Stresses such as cold and drought can impair plant yield and induce a highly complex array of responses. Sugarcane (Saccharum spp. is cultivated in tropical and subtropical areas and is considered a cold-sensitive plant. We previously showed that cold stress induces the expression of several genes in in vitro sugarcane plantlets. Here we characterize one of those genes, SsNAC23, a member of the NAC family of plant-specific transcription factors, which are induced by low temperature and other stresses in several plant species. The expression of SsNAC23 was induced in sugarcane plants exposed to low temperatures (4ºC. With the aim of further understanding the regulatory network in response to stress, we used the yeast two-hybrid system to identify sugarcane proteins that interact with SsNAC23. Using SsNAC23 as bait, we screened a cDNA expression library of sugarcane plants submitted to 4ºC for 48 h. Several interacting partners were identified, including stress-related proteins, increasing our knowledge on how sugarcane plants respond to cold stress. One of these interacting partners, a thioredoxin h1, offers insights into the regulation of SsNAC23 activity.

Excessive counterion condensation on immobilized ssDNA in solutions of high ionic strength.

Science.gov (United States)

Rant, Ulrich; Arinaga, Kenji; Fujiwara, Tsuyoshi; Fujita, Shozo; Tornow, Marc; Yokoyama, Naoki; Abstreiter, Gerhard

2003-12-01

We present experiments on the bias-induced release of immobilized, single-stranded (ss) 24-mer oligonucleotides from Au-surfaces into electrolyte solutions of varying ionic strength. Desorption is evidenced by fluorescence measurements of dye-labeled ssDNA. Electrostatic interactions between adsorbed ssDNA and the Au-surface are investigated with respect to 1), a variation of the bias potential applied to the Au-electrode; and 2), the screening effect of the electrolyte solution. For the latter, the concentration of monovalent salt in solution is varied from 3 to 1600 mM. We find that the strength of electric interaction is predominantly determined by the effective charge of the ssDNA itself and that the release of DNA mainly occurs before the electrochemical double layer has been established at the electrolyte/Au interface. In agreement with Manning's condensation theory, the measured desorption efficiency (etarel) stays constant over a wide range of salt concentrations; however, as the Debye length is reduced below a value comparable to the axial charge spacing of the DNA, etarel decreases substantially. We assign this effect to excessive counterion condensation on the DNA in solutions of high ionic strength. In addition, the relative translational diffusion coefficient of ssDNA in solution is evaluated for different salt concentrations.

Oral Treatment with Extract of Agaricus blazei Murill Enhanced Th1 Response through Intestinal Epithelial Cells and Suppressed OVA-Sensitized Allergy in Mice

Directory of Open Access Journals (Sweden)

Go Bouike

2011-01-01

Full Text Available To clarify the mechanism of the antiallergic activity of Agaricus blazei Murill extract (ABME, the present paper used an in vivo allergy model and an in vitro intestinal gut model. During OVA sensitization, the serum IgE levels decreased significantly in ABME group. Interleukin (IL-4 and -5 produced from OVA-restimulated splenocytes was significantly decreased, and anti-CD3ε/CD28 antibody treatment also reduced IL-10, -4, and -5 production and increased IFN-γ production in ABME group. These results suggest that oral administration of ABME improves Th1/Th2 balance. Moreover, a coculture system constructed of Caco-2 cells and splenocytes from OT-II mice or RAW 264.7 cells indicated that the significant increases in IFN-γ production by ABME treatment. Therefore, it was concluded that the antiallergic activity of ABME was due to the activation of macrophages by epithelial cells and the promotion of the differentiation of naïve T cells into Th1 cells in the immune.

Modulación de la expresión por GA y ABA de los genes Ss1 y Ss2 que codifican sacarosa sintasa en cebada

OpenAIRE

Carbonero Zalduegui, Pilar; Barrero Sicilia, Cristina; Oñate Sanchez, Luis; Hernando Amado, Sara; Rueda Romero, Paloma

2008-01-01

En este trabajo se ha llevado a cabo un estudio comparativo entre distintas isoformas de SUSy de cereales y arabidopsis. Además se ha realizado un análisis de expresión de HvSs1 y HvSs2 en distintos órganos, incluyendo patrones temporales en semillas en desarrollo y germinación, así como la variación de su respuesta a ácido abscísico (ABA) y giberélico (GA3).

Photometric variability of the SS 433 (V 1343 Aq1) in 1979-1981

International Nuclear Information System (INIS)

Gladyshev, S.A.; Goranskij, V.P.; Cherepashchuk, A.M.

1983-01-01

Results of photometric observations of SS 433 carried out in 1981 are presented, the 164-day and 13-day variability of this ob ect being confirmed. All available photometric observations of SS 433 during 1979-1981 are summarized in order to investigate main regularities in brightness variability. Using the photometric data of three years the precessing period of 164sup(d)+-1sup(d) is determined. The orbital period is 13sup(d).086+-0sup(d).006. The maximum of the uneclipsed brightness of SS 433 is observed at the moment of maximum separation of ''relativistic'' emission lines. At this moment the orbital brightness curve is most similar to that of #betta# Lyr-type eclipsing variables. The amplitude of the orbital 13-day variability is minimal near the moments of coincidence of relativistic emissions (''cross-over'' dates). New observational data confirm the model of SS 433 as a massive eclipsing binary with a ''slaved'' bright accretion disk

Effect of water vapor on annealing scale formation on 316 SS

International Nuclear Information System (INIS)

Cheng, S.-Y.; Kuan, S.-L.; Tsai, W.-T.

2006-01-01

The oxidation behavior of 316 stainless steel (SS) annealed in air containing 0.1 atm water vapor at temperatures ranging from 800 to 1030 deg. C was investigated. A kinetic study of the oxidation was made by employing thermal-gravimetric analysis (TGA). The morphology, composition and structure of the scale were examined by scanning electron microscopy (SEM) and X-ray diffraction (XRD). The experimental results showed that significant breakaway oxidation occurred, resulting in substantial weight increase, as the steel was annealed in moist air at temperatures above 950 deg. C. The scaling behavior of 316 SS in wet air at 1030 deg. C could be divided into two stages based on the alteration of the oxidation rate. In each stage, the scale on 316 SS exhibited a different structure and morphology. The complex process of the formation of scale in wet air was discussed and proposed

Low-molecular-weight polysaccharides from Agaricus blazei Murrill modulate the Th1 response in cancer immunity

Science.gov (United States)

Jiang, Liyan; Yu, Zhipu; Lin, Yu; Cui, Liran; Yao, Shujuan; Lv, Liyan; Liu, Jicheng

2018-01-01

To assess the effect of low-molecular-weight polysaccharides from Agaricus blazei Murrill (ABP-AW1) as an immunoadjuvant therapy for type 1 T-helper (Th1) responses in tumorigenesis, C57BL/6 mice were inoculated subcutaneously with ovalbumin (E.G7-OVA). After 3, 10 and 17 days, the mice were immunized with PBS, OVA alone, or OVA and ABP-AW1, at low (50 µg), intermediate (100 µg) or high (200 µg) doses. Tumor growth was examined and compared among the groups, as were the following parameters: Splenocyte viability/proliferation, peripheral blood CD4+/CD8+ T cell ratio, serum OVA-specific IgG1 and IgG2b, secretion of interleukin (IL)-2 and interferon (IFN)-γ, and IFN-γ production on a single cell level from cultured splenocytes. Tumor growth in mice treated with OVA and ABP-AW1 (100 or 200 µg) was significantly slower, compared with in the other groups at the same time-points. OVA with 100 or 200 µg ABP-AW1 was associated with a higher number of total splenocytes, a higher ratio of peripheral blood CD4+/CD8+ T-lymphocytes, higher serum levels of OVA-specific Th1-type antibody IgG2b and greater secretion of the Th1 cytokines IL-1 and IFN-γ from splenocytes. ABP-AW1 is a promising immunoadjuvant therapy candidate, due to its ability to boost the Th1 immune response when co-administered with a cancer vaccine intended to inhibit cancer progression. PMID:29467867

Dietary supplementation with Agaricus blazei murill extract prevents diet-induced obesity and insulin resistance in rats.

Science.gov (United States)

Vincent, Mylène; Philippe, Erwann; Everard, Amandine; Kassis, Nadim; Rouch, Claude; Denom, Jessica; Takeda, Yorihiko; Uchiyama, Shoji; Delzenne, Nathalie M; Cani, Patrice D; Migrenne, Stéphanie; Magnan, Christophe

2013-03-01

Dietary supplement may potentially help to fight obesity and other metabolic disorders such as insulin-resistance and low-grade inflammation. The present study aimed to test whether supplementation with Agaricus blazei murill (ABM) extract could have an effect on diet-induced obesity in rats. Wistar rats were fed with control diet (CD) or high-fat diet (HF) and either with or without supplemented ABM for 20 weeks. HF diet-induced body weight gain and increased fat mass compared to CD. In addition HF-fed rats developed hyperleptinemia and insulinemia as well as insulin resistance and glucose intolerance. In HF-fed rats, visceral adipose tissue also expressed biomarkers of inflammation. ABM supplementation in HF rats had a protective effect against body weight gain and all study related disorders. This was not due to decreased food intake which remained significantly higher in HF rats whether supplemented with ABM or not compared to control. There was also no change in gut microbiota composition in HF supplemented with ABM. Interestingly, ABM supplementation induced an increase in both energy expenditure and locomotor activity which could partially explain its protective effect against diet-induced obesity. In addition a decrease in pancreatic lipase activity is also observed in jejunum of ABM-treated rats suggesting a decrease in lipid absorption. Taken together these data highlight a role for ABM to prevent body weight gain and related disorders in peripheral targets independently of effect in food intake in central nervous system. Copyright © 2012 The Obesity Society.

Comparison of the microbial communities in solid-state anaerobic digestion (SS-AD) reactors operated at mesophilic and thermophilic temperatures.

Science.gov (United States)

Li, Yueh-Fen; Nelson, Michael C; Chen, Po-Hsu; Graf, Joerg; Li, Yebo; Yu, Zhongtang

2015-01-01

The microbiomes involved in liquid anaerobic digestion process have been investigated extensively, but the microbiomes underpinning solid-state anaerobic digestion (SS-AD) are poorly understood. In this study, microbiome composition and temporal succession in batch SS-AD reactors, operated at mesophilic or thermophilic temperatures, were investigated using Illumina sequencing of 16S rRNA gene amplicons. A greater microbial richness and evenness were found in the mesophilic than in the thermophilic SS-AD reactors. Firmicutes accounted for 60 and 82 % of the total Bacteria in the mesophilic and in the thermophilic SS-AD reactors, respectively. The genus Methanothermobacter dominated the Archaea in the thermophilic SS-AD reactors, while Methanoculleus predominated in the mesophilic SS-AD reactors. Interestingly, the data suggest syntrophic acetate oxidation coupled with hydrogenotrophic methanogenesis as an important pathway for biogas production during the thermophilic SS-AD. Canonical correspondence analysis (CCA) showed that temperature was the most influential factor in shaping the microbiomes in the SS-AD reactors. Thermotogae showed strong positive correlation with operation temperature, while Fibrobacteres, Lentisphaerae, Spirochaetes, and Tenericutes were positively correlated with daily biogas yield. This study provided new insight into the microbiome that drives SS-AD process, and the findings may help advance understanding of the microbiome in SS-AD reactors and the design and operation of SS-AD systems.

Safe and effective error rate monitors for SS7 signaling links

Science.gov (United States)

Schmidt, Douglas C.

1994-04-01

This paper describes SS7 error monitor characteristics, discusses the existing SUERM (Signal Unit Error Rate Monitor), and develops the recently proposed EIM (Error Interval Monitor) for higher speed SS7 links. A SS7 error monitor is considered safe if it ensures acceptable link quality and is considered effective if it is tolerant to short-term phenomena. Formal criteria for safe and effective error monitors are formulated in this paper. This paper develops models of changeover transients, the unstable component of queue length resulting from errors. These models are in the form of recursive digital filters. Time is divided into sequential intervals. The filter's input is the number of errors which have occurred in each interval. The output is the corresponding change in transmit queue length. Engineered EIM's are constructed by comparing an estimated changeover transient with a threshold T using a transient model modified to enforce SS7 standards. When this estimate exceeds T, a changeover will be initiated and the link will be removed from service. EIM's can be differentiated from SUERM by the fact that EIM's monitor errors over an interval while SUERM's count errored messages. EIM's offer several advantages over SUERM's, including the fact that they are safe and effective, impose uniform standards in link quality, are easily implemented, and make minimal use of real-time resources.

Screening and Identification of ssDNA Aptamer for Human GP73

Directory of Open Access Journals (Sweden)

Jingchun Du

2015-01-01

Full Text Available As one tumor marker of HCC, Golgi Protein 73 (GP73 is given more promise in the early diagnosis of HCC, and aptamers have been developed to compete with antibodies as biorecognition probes in different detection system. In this study, we utilized GP73 to screen specific ssDNA aptamers by SELEX technique. First, GP73 proteins were expressed and purified by prokaryotic expression system and Nickle ion affinity chromatography, respectively. At the same time, the immunogenicity of purified GP73 was confirmed by Western blotting. The enriched ssDNA library with high binding capacity for GP73 was obtained after ten rounds of SELEX. Then, thirty ssDNA aptamers were sequenced, in which two ssDNA aptamers with identical DNA sequence were confirmed, based on the alignment results, and designated as A10-2. Furthermore, the specific antibody could block the binding of A10-2 to GP73, and the specific binding of A10-2 to GP73 was also supported by the observation that several tumor cell lines exhibited variable expression level of GP73. Significantly, the identified aptamer A10-2 could distinguish normal and cancerous liver tissues. So, our results indicate that the aptamer A10-2 might be developed into one molecular probe to detect HCC from normal liver specimens.

Binding of antibodies to the extractable nuclear antigens SS-A/Ro and SS-B/La is induced on the surface of human keratinocytes by ultraviolet light (UVL): Implications for the pathogenesis of photosensitive cutaneous lupus

International Nuclear Information System (INIS)

Furukawa, F.; Kashihara-Sawami, M.; Lyons, M.B.; Norris, D.A.

1990-01-01

Autoantibodies to the non-histone nucleoprotein antigens SS-A/Ro, SS-B/La, and RNP are highly associated with photosensitive cutaneous lupus erythematosus (LE). In order to better understand the potential mechanisms of ultraviolet (UV) light on photosensitivity in patients with cutaneous LE, we designed immunopathologic in vitro and in vivo experiments to evaluate the effects of UV on the binding of such autoantibodies to the surface of human keratinocytes, one major target of immunologic damage in photosensitive LE. Short-term 2% paraformaldehyde fixation of suspensions of cultured human keratinocytes previously incubated with monospecific antiserum probes enabled the detection of ENA expression on the cell surface by flow-cytometry analysis. UVB light (280-320 nm) induced the binding of monospecific antibody probes for SS-A/Ro and SS-B/La on keratinocytes in a dose-dependent pattern with maximal induction observed at the dose of 200 mJ/cm2 UVB. Binding of SS-A/Ro, SS-B/La, and RNP antibody was augmented strongly, but binding of anti-Sm was very weak. In contrast, UVA (320-400 nm) light had no effect on the induction of binding of these antibody probes. Identical results were seen by standard immunofluorescence techniques. Hydroxyurea-treated keratinocytes showed similar induction of those antigens by UVB irradiation, which suggested that ENA expression on cultured keratinocytes by UVB were cell-cycle independent. Tunicamycin, an inhibitor of glycosylation of proteins, reduced UVB light effect on the SS-A/Ro and SS-B/La antigen's expression. These in vitro FACS analyses revealed that ENA augmentation on the keratinocyte cell surface was dose dependent, UVB dependent, glycosylation dependent, and cell-cycle independent. In vivo ENA augmentation on the keratinocyte surface was examined in suction blister epidermal roofs

Blood parameters of sheep with high infection of Haemonchus contortus and treated with “mushroom of the sun” (Agaricus blazei

Directory of Open Access Journals (Sweden)

Gabriela Almeida Bastos

2016-04-01

Full Text Available Blood and parasitological parameters of sheep experimentally infected with Haemonchus contortus and treated with “mushroom of the sun” (Agaricus blazei were assessed. Lambs infected with the nematode were divided into three groups: treated with basidiocarp powder from the fungus, wormed with trichlorfon, and a control group that did not receive anthelmintic. Split-plot design analysis was performed where the treatments were defined as plots and four periods of collection were defined as subplots. Interaction between the treatments and the collection periods for the fecal egg counts per gram of feces (FEC was observed. Treatment with “mushroom of the sun” showed anthelmintic efficacies ranging from 28.6 to 54.2%. Similar performances to weight gain were observed among the groups. A significant interaction between treatments and evaluation periods was observed for erythrocyte counts, hematocrit value, and serum concentrations of albumin and urea. The values of the mean corpuscular volume, erythrocyte distribution width, and leukocyte, neutrophil, lymphocyte, monocyte, and eosinophil counts, as well as the mean platelet volume, varied among the collection days. The administration of “mushroom of the sun” significantly reduced the FEC, did not influence on the erythrocyte count and hematocrit value, which remained within normal limits. However it increased the serum concentrations of albumin.

Single-molecule analysis reveals the kinetics and physiological relevance of MutL-ssDNA binding.

Directory of Open Access Journals (Sweden)

Jonghyun Park

2010-11-01

Full Text Available DNA binding by MutL homologs (MLH/PMS during mismatch repair (MMR has been considered based on biochemical and genetic studies. Bulk studies with MutL and its yeast homologs Mlh1-Pms1 have suggested an integral role for a single-stranded DNA (ssDNA binding activity during MMR. We have developed single-molecule Förster resonance energy transfer (smFRET and a single-molecule DNA flow-extension assays to examine MutL interaction with ssDNA in real time. The smFRET assay allowed us to observe MutL-ssDNA association and dissociation. We determined that MutL-ssDNA binding required ATP and was the greatest at ionic strength below 25 mM (K(D = 29 nM while it dramatically decreases above 100 mM (K(D>2 µM. Single-molecule DNA flow-extension analysis suggests that multiple MutL proteins may bind ssDNA at low ionic strength but this activity does not enhance stability at elevated ionic strengths. These studies are consistent with the conclusion that a stable MutL-ssDNA interaction is unlikely to occur at physiological salt eliminating a number of MMR models. However, the activity may infer some related dynamic DNA transaction process during MMR.

Characterization of 316L(N)-IG SS joint produced by hot isostatic pressing technique

International Nuclear Information System (INIS)

Nakano, J.; Miwa, Y.; Tsukada, T.; Kikuchi, M.; Kita, S.; Nemoto, Y.; Tsuji, H.; Jitsukawa, S.

2002-01-01

Type 316L(N) stainless steel of the international thermonuclear experimental reactor grade (316L(N)-IG SS) is being considered for the first wall/blanket module. Hot isostatic pressing (HIP) technique is expected for the fabrication of the module. To evaluate the integrity and susceptibility to stress corrosion cracking (SCC) of HIPed 316L(N)-IG SS, tensile tests in vacuum and slow strain rate tests in high temperature water were performed. Specimen with the HIPed joint had similar tensile properties to specimens of 316L(N)-IG SS, and did not show susceptibility to SCC in oxygenated water at 423 K. Thermally sensitized specimen was low susceptible to SCC even in the creviced condition. It is concluded that the tensile properties of HIPed SS are as high as those of the base alloy and the HIP process caused no deleterious effects

Fast Ss-Ilm a Computationally Efficient Algorithm to Discover Socially Important Locations

Science.gov (United States)

Dokuz, A. S.; Celik, M.

2017-11-01

Socially important locations are places which are frequently visited by social media users in their social media lifetime. Discovering socially important locations provide several valuable information about user behaviours on social media networking sites. However, discovering socially important locations are challenging due to data volume and dimensions, spatial and temporal calculations, location sparseness in social media datasets, and inefficiency of current algorithms. In the literature, several studies are conducted to discover important locations, however, the proposed approaches do not work in computationally efficient manner. In this study, we propose Fast SS-ILM algorithm by modifying the algorithm of SS-ILM to mine socially important locations efficiently. Experimental results show that proposed Fast SS-ILM algorithm decreases execution time of socially important locations discovery process up to 20 %.

FAST SS-ILM: A COMPUTATIONALLY EFFICIENT ALGORITHM TO DISCOVER SOCIALLY IMPORTANT LOCATIONS

Directory of Open Access Journals (Sweden)

A. S. Dokuz

2017-11-01

Full Text Available Socially important locations are places which are frequently visited by social media users in their social media lifetime. Discovering socially important locations provide several valuable information about user behaviours on social media networking sites. However, discovering socially important locations are challenging due to data volume and dimensions, spatial and temporal calculations, location sparseness in social media datasets, and inefficiency of current algorithms. In the literature, several studies are conducted to discover important locations, however, the proposed approaches do not work in computationally efficient manner. In this study, we propose Fast SS-ILM algorithm by modifying the algorithm of SS-ILM to mine socially important locations efficiently. Experimental results show that proposed Fast SS-ILM algorithm decreases execution time of socially important locations discovery process up to 20 %.

Protein dynamics during presynaptic complex assembly on individual ssDNA molecules

Science.gov (United States)

Gibb, Bryan; Ye, Ling F.; Kwon, YoungHo; Niu, Hengyao; Sung, Patrick; Greene, Eric C.

2014-01-01

Homologous recombination is a conserved pathway for repairing double–stranded breaks, which are processed to yield single–stranded DNA overhangs that serve as platforms for presynaptic complex assembly. Here we use single–molecule imaging to reveal the interplay between Saccharomyce cerevisiae RPA, Rad52, and Rad51 during presynaptic complex assembly. We show that Rad52 binds RPA–ssDNA and suppresses RPA turnover, highlighting an unanticipated regulatory influence on protein dynamics. Rad51 binding extends the ssDNA, and Rad52–RPA clusters remain interspersed along the presynaptic complex. These clusters promote additional binding of RPA and Rad52. Together, our work illustrates the spatial and temporal progression of RPA and Rad52 association with the presynaptic complex, and reveals a novel RPA–Rad52–Rad51–ssDNA intermediate, which has implications for understanding how the activities of Rad52 and RPA are coordinated with Rad51 during the later stages recombination. PMID:25195049

Suklaajälkiruoat ja niiden valmistamisen haasteet ammattikeittiössä

OpenAIRE

Lappalainen, Arto

2016-01-01

Tämä opinnäytetyö on osa palvelujen tuottamisen ja johtamisen koulutusohjelmaa. Opinnäytetyön aiheena on suklaajälkiruuat ja niiden valmistamisen haasteet ammattikeittiössä. Opinnäytetyön teoriaosuudessa käsitellään suklaan historiaa, lajikkeita, tuottajamaita, valmistusmenetelmiä, suklaan laatuja, tärkeimpiä valmistajia sekä suklaan käyttöä ja tekniikoita ammattikeittiössä. Tutkimuksessa selvitettiin yleisimpiä suklaajälkiruokia ja niiden valmistuksessa olevia haasteita ammattikeitti...

GSM SiNYALLERİNİN .IP OMURGASI İİZERiNI>E TAŞINMASI (SS7 OVER IP- NettcSS7)

OpenAIRE

Tülü, Çağatay Neftali; Demirkol, Aşkın

2003-01-01

Bu çalışmada günümüzün en popüler nıobilhaberleşme sistemi olan GSM [1 l sisteminin, kendiöğeleri aras1ndaki en temel sinyalleşme sistemi olannumara 7 (SS7 [2) ) sinyallerinin, günümüzün enyaygın iletim omurgast olan ve tüm dünyadakibilgisayarları birbirine bağlayan IP (3] omurgasıüzerinden taşınabilmesi için gerekli si stemin tasar11n1ve gerçeklenınesi üzerinde çahş1lmtştır. Klasil\\ SS7iletimine göre en büyül< faydası çok düşül{ maliyetiolan bu sistem sayesinde, dünyadaki b i r çok GS...

Fluorescence Detection and Discrimination of ss- and ds-DNA with a Water Soluble Oligopyrene Derivative

Directory of Open Access Journals (Sweden)

Gaoquan Shi

2009-06-01

Full Text Available A novel water-soluble cationic conjugated oligopyrene derivative, oligo(N1,N1,N1,N4,N4,N4-hexamethyl-2-(4-(pyren-1-yl butane-1,4-diaminium bromide (OHPBDB, was synthesized by a combination of chemical and electrochemical synthesis techniques. Each oligomer chain has five pyrene derivative repeating units and brings 10 positive charges. OHPBDB showed high and rapid fluorescence quenching in aqueous media upon addition of trace amounts of single-stranded (ss and double-stranded (ds DNA. The Stern-Volmer constants for ss- and ds-DNA were measured to be as high as 1.3 × 108 mol-1·L and 1.2 × 108 mol-1·L, respectively. On the other hand, distinct fluorescence enhancement of OHPBDB upon addition of large amount of ss-DNA or ds-DNA was observed. Furthermore, ss-DNA showed much stronger fluorescence enhancement than that of ds-DNA, thus yielding a clear and simple signal useful for the discrimination between ss- and ds-DNA in aqueous media.

Serum testosterone levels of HbSS (sickle cell disease male subjects in Lagos, Nigeria

Directory of Open Access Journals (Sweden)

Adediran Adewumi

2011-08-01

Full Text Available Abstract Background Infertility is a major problem in sickle cell disease patients, especially in males. In addition to low serum testosterone, other abnormalities involving the accessory sex organs, such as the seminal vesicles and the prostate gland, as well as marked decrease in ejaculate volume may be observed in male HbSS patients. Hence, the need to study the role of sex hormones as a cause of infertility in male HbSS patients. Methods An unmatched case-control study was performed using seventy-five consenting subjects from Lagos University Teaching Hospital. These included 47 patients with haemoglobin phenotype SS from the Sickle cell clinic and 28 volunteered medical students and members of staff with haemoglobin phenotype AA. Demographic data were obtained using a self-administered questionnaire. A total of 5 mls of blood was collected from each subject between 9.00 am & 11.am, and assayed for serum testosterone concentration. Results The concentrations of serum testosterone in HbSS patients ranged from 0.2 to 4.3 ng/ml with a mean of 1.28 ± 0.72 ng/ml whilst the values in HbAA controls ranged from 1.2 to 6.9 ng/ml with a mean of 2.63 ± 1.04 ng/ml. Seven (25.0% of the 28 controls had serum testosterone concentration lower than the quoted reference (normal range whereas 44 (93.6% of the 47 HbSS subjects had serum testosterone concentration lower than the reference range. Conclusion Overall, subjects with HbSS have significantly lower mean serum testosterone than HbAA controls.

Kehityshankkeena tasa-arvo : Sukupuolinäkökulman huomioon ottaminen teknologiaosaamisen johtamisessa Yritys Oyj:ssä

OpenAIRE

Syrjäläinen, Suvi

2010-01-01

Tämä opinnäytetyö tehtiin suurelle kansainväliselle teknologiayritykselle, josta käytän tässä opinnäytetyössä työssä nimeä Yritys Oyj. Opinnäytetyön johtoajatuksena oli tehdä suomalainen sukupuolijärjestelmä, -stereotypiat sekä sukupuolten välinen tasa-arvo näkyväksi ja osoittaa, kuinka näitä parantamalla voidaan lisätä Yritys Oyj:n tuottavuutta ja sitä kautta menestystä. Työn sukupuolta ja sukupuolieroja käsittelevässä teoriaosuudessa selvitettiin sukupuolistereotypioiden olemassao...

Structure/property (constitutive and dynamic strength/damage) characterization of additively manufactured 316L SS

Science.gov (United States)

Gray, G. T., III; Livescu, V.; Rigg, P. A.; Trujillo, C. P.; Cady, C. M.; Chen, S. R.; Carpenter, J. S.; Lienert, T. J.; Fensin, S.

2015-09-01

For additive manufacturing (AM), the certification and qualification paradigm needs to evolve as there exists no "ASTM-type" additive manufacturing certified process or AM-material produced specifications. Accordingly, utilization of AM materials to meet engineering applications requires quantification of the constitutive properties of these evolving materials in comparison to conventionally-manufactured metals and alloys. Cylinders of 316L SS were produced using a LENS MR-7 laser additive manufacturing system from Optomec (Albuquerque, NM) equipped with a 1kW Yb-fiber laser. The microstructure of the AM-316L SS is detailed in both the as-built condition and following heat-treatments designed to obtain full recrystallization. The constitutive behavior as a function of strain rate and temperature is presented and compared to that of nominal annealed wrought 316L SS plate. The dynamic damage evolution and failure response of all three materials was probed using flyer-plate impact driven spallation experiments at a peak stress of 4.5 GPa to examine incipient spallation response. The spall strength of AM-produced 316L SS was found to be very similar for the peak shock stress studied to that of annealed wrought or AM-316L SS following recrystallization. The damage evolution as a function of microstructure was characterized using optical metallography.

Analysis of T4SS-induced signaling by H. pylori using quantitative phosphoproteomics

Directory of Open Access Journals (Sweden)

Frithjof eGlowinski

2014-07-01

Full Text Available Helicobacter pylori is a Gram-negative bacterial pathogen colonizing the human stomach. Infection with H. pylori causes chronic inflammation of the gastric mucosa and may lead to peptic ulceration and/or gastric cancer. A major virulence determinant of H. pylori is the type IV secretion system (T4SS, which is used to inject the virulence factor CagA into the host cell, triggering a wide range of cellular signaling events. Here, we used a phosphoproteomic approach to investigate tyrosine signaling in response to host-pathogen interaction, using stable isotope labeling in cell culture (SILAC of AGS cells to obtain a differential picture between multiple infection conditions. Cells were infected with wild type H. pylori P12, a P12ΔCagA deletion mutant, and a P12ΔT4SS deletion mutant to compare signaling changes over time and in the absence of CagA or the T4SS. Tryptic peptides were enriched for tyrosine (Tyr phosphopeptides and analysed by nano-LC-Orbitrap MS. In total, 58 different phosphosites were found to be regulated following infection. The majority of phosphosites identified were kinases of the MAPK familiy. CagA and the T4SS were found to be key regulators of Tyr phosphosites. Our findings indicate that CagA primarily induces activation of ERK1 and integrin linked factors, whereas the T4SS primarily modulates JNK and p38 activation.

Attenuation of the in vitro neurotoxicity of 316L SS by graphene oxide surface coating

International Nuclear Information System (INIS)

Tasnim, Nishat; Kumar, Alok; Joddar, Binata

2017-01-01

A persistent theme in biomaterials research comprises of surface engineering and modification of bare metallic substrates for improved cellular response and biocompatibility. Graphene Oxide (GO), a derivative of graphene, has outstanding chemical and mechanical properties; its large surface to volume ratio, ease of surface modification and processing make GO an attractive coating material. GO-coatings have been extensively studied as biosensors. Further owing to its surface nano-architecture, GO-coated surfaces promote cell adhesion and growth, making it suitable for tissue engineering applications. The need to improve the long-term durability and therapeutic effectiveness of commercially available bare 316L stainless steel (SS) surfaces led us to adopt a polymer-free approach which is cost-effective and scalable. GO was immobilized on to 316L SS utilizing amide linkage, to generate a strongly adherent uniform coating with surface roughness. GO-coated 316L SS surfaces showed increased hydrophilicity and biocompatibility with SHSY-5Y neuronal cells, which proliferated well and showed decreased reactive oxygen species (ROS) expression. In contrast, cells did not adhere to bare uncoated 316L SS meshes nor maintain viability when cultured in the vicinity of bare meshes. Therefore the combination of the improved surface properties and biocompatibility implies that GO-coating can be utilized to overcome pertinent limitations of bare metallic 316L SS implant surfaces, especially SS neural electrodes. Also, the procedure for making GO-based protective coatings can be applied to numerous other implants where the development of such protective films is necessary. - Highlights: • GO was immobilized on to 316L SS utilizing carbodiimide chemistry to generate a strong adherent uniform nano coating. • GO-modified surfaces showed increased hydrophilicity and biocompatibility with SH5YSY cells cultured atop these surfaces. • Proliferation and alignment of the cells with the

Attenuation of the in vitro neurotoxicity of 316L SS by graphene oxide surface coating

Energy Technology Data Exchange (ETDEWEB)

Tasnim, Nishat; Kumar, Alok; Joddar, Binata, E-mail: bjoddar@utep.edu

2017-04-01

A persistent theme in biomaterials research comprises of surface engineering and modification of bare metallic substrates for improved cellular response and biocompatibility. Graphene Oxide (GO), a derivative of graphene, has outstanding chemical and mechanical properties; its large surface to volume ratio, ease of surface modification and processing make GO an attractive coating material. GO-coatings have been extensively studied as biosensors. Further owing to its surface nano-architecture, GO-coated surfaces promote cell adhesion and growth, making it suitable for tissue engineering applications. The need to improve the long-term durability and therapeutic effectiveness of commercially available bare 316L stainless steel (SS) surfaces led us to adopt a polymer-free approach which is cost-effective and scalable. GO was immobilized on to 316L SS utilizing amide linkage, to generate a strongly adherent uniform coating with surface roughness. GO-coated 316L SS surfaces showed increased hydrophilicity and biocompatibility with SHSY-5Y neuronal cells, which proliferated well and showed decreased reactive oxygen species (ROS) expression. In contrast, cells did not adhere to bare uncoated 316L SS meshes nor maintain viability when cultured in the vicinity of bare meshes. Therefore the combination of the improved surface properties and biocompatibility implies that GO-coating can be utilized to overcome pertinent limitations of bare metallic 316L SS implant surfaces, especially SS neural electrodes. Also, the procedure for making GO-based protective coatings can be applied to numerous other implants where the development of such protective films is necessary. - Highlights: • GO was immobilized on to 316L SS utilizing carbodiimide chemistry to generate a strong adherent uniform nano coating. • GO-modified surfaces showed increased hydrophilicity and biocompatibility with SH5YSY cells cultured atop these surfaces. • Proliferation and alignment of the cells with the

Skipping Strategy (SS) for Initial Population of Job-Shop Scheduling Problem

Science.gov (United States)

Abdolrazzagh-Nezhad, M.; Nababan, E. B.; Sarim, H. M.

2018-03-01

Initial population in job-shop scheduling problem (JSSP) is an essential step to obtain near optimal solution. Techniques used to solve JSSP are computationally demanding. Skipping strategy (SS) is employed to acquire initial population after sequence of job on machine and sequence of operations (expressed in Plates-jobs and mPlates-jobs) are determined. The proposed technique is applied to benchmark datasets and the results are compared to that of other initialization techniques. It is shown that the initial population obtained from the SS approach could generate optimal solution.

Susceptibility to Stress Corrosion Cracking of 254SMO SS

Directory of Open Access Journals (Sweden)

De Micheli Lorenzo

2002-01-01

Full Text Available The susceptibility to stress corrosion cracking (SCC of solubilized and sensitized 254SMO SS was studied in sodium chloride, and sodium fluoride solutions at 80 °C and sulfuric acid solutions in presence of sodium chloride at 25 °C. The influence of salt concentration, pH values and the addition of thiosulfate was examined. The susceptibility to SCC was evaluated by Slow Strain Rate Tests (SSRT, at 1.5 x 10-6 s-1 strain rate. The behavior of 254SMO was compared to those of AISI 316L SS and Hastelloy C276. 254SMO showed an excellent resistance to SCC in all conditions, except in the more acidic solutions (pH <= 1 where, in the sensitized conditions, intergranular stress corrosion cracking occurred.

Characterization of systemic disease in primary Sjogren's syndrome : EULAR-SS Task Force recommendations for articular, cutaneous, pulmonary and renal involvements

NARCIS (Netherlands)

Ramos-Casals, Manuel; Brito-Zeron, Pilar; Seror, Raphaele; Bootsma, Hendrika; Bowman, Simon J.; Doerner, Thomas; Gottenberg, Jacques-Eric; Mariette, Xavier; Theander, Elke; Bombardieri, Stefano; De Vita, Salvatore; Mandl, Thomas; Ng, Wan-Fai; Kruize, Aike; Tzioufas, Athanasios; Vitali, Claudio

2015-01-01

Objective. To reach a European consensus on the definition and characterization of the main organspecific extraglandular manifestations in primary SS. Methods. The EULAR-SS Task Force Group steering committee agreed to approach SS-related systemic involvement according to the EULAR SS Disease

Phase transformation and hardness of SS 316 L steel cast alloy after heat treatment at high temperature

International Nuclear Information System (INIS)

Hidayat, S.; Prayitno, D. H.

2000-01-01

Heat treatment Study of SS 316 L cast alloy at high temperature was conducted. The alloy of SS 316 L was melted by arc melting furnace in argon atmosphere. Heat treatment of SS 316 L casting alloy was carried out in tube furnace at 1400 o C for period of 1/2, 1, and 2 hours. The optical microscopic characterization showed that SS 316 L cast has got dendritic micro structure with ferrite as the primary phase. After the heat treatment, the ferrite phase underwent gradual decrease followed by an increase of the austenite phase. The heat treatment process also resulted in the formation of the new grain boundary. The hardness examination revealed that for longer period of the heat treatment, the hardness of SS 316 L increased. (author)

An investigation of p-normed sparsity evaluation for SS-LBP-based edge extraction

Energy Technology Data Exchange (ETDEWEB)

Chen-yi, Zhao [School of Physics, Northeast Normal University, Changchun (China); Jia-ning, Sun, E-mail: sunjn118@nenu.edu.cn [School of Mathematics and Statistics, Northeast Normal University, Changchun (China); Shuang, Qiao, E-mail: qiaos810@nenu.edu.cn [School of Physics, Northeast Normal University, Changchun (China)

2017-02-01

SS-LBP is a very efficient tool for fast edge extraction in digital radiography. In this paper, we introduce a p-normed sparsity evaluation strategy to improve and generalize the existing SS-LBP framework. To illustrate the feasibility of the proposed approach, several experimental results are presented. Comparisons show that 1-normed sparsity evaluation is more effective and robust in practical applications.

Subtle variation within conserved effector operon gene products contributes to T6SS-mediated killing and immunity.

Science.gov (United States)

Alteri, Christopher J; Himpsl, Stephanie D; Zhu, Kevin; Hershey, Haley L; Musili, Ninette; Miller, Jessa E; Mobley, Harry L T

2017-11-01

Type VI secretion systems (T6SS) function to deliver lethal payloads into target cells. Many studies have shown that protection against a single, lethal T6SS effector protein requires a cognate antidote immunity protein, both of which are often encoded together in a two-gene operon. The T6SS and an effector-immunity pair is sufficient for both killing and immunity. HereIn this paper we describe a T6SS effector operon that differs from conventional effector-immunity pairs in that eight genes are necessary for lethal effector function, yet can be countered by a single immunity protein. In this study, we investigated the role that the PefE T6SS immunity protein plays in recognition between two strains harboring nearly identical effector operons. Interestingly, despite containing seven of eight identical effector proteins, the less conserved immunity proteins only provided protection against their native effectors, suggesting that specificity and recognition could be dependent on variation within an immunity protein and one effector gene product. The variable effector gene product, PefD, is encoded upstream from pefE, and displays toxic activity that can be countered by PefE independent of T6SS-activity. Interestingly, while the entire pef operon was necessary to exert toxic activity via the T6SS in P. mirabilis, production of PefD and PefE alone was unable to exert this effector activity. Chimeric PefE proteins constructed from two P. mirabilis strains were used to localize immunity function to three amino acids. A promiscuous immunity protein was created using site-directed mutagenesis to change these residues from one variant to another. These findings support the notion that subtle differences between conserved effectors are sufficient for T6SS-mediated kin discrimination and that PefD requires additional factors to function as a T6SS-dependent effector.

Nucleation and growth microstructural study of ti films on 304 SS substrates

Directory of Open Access Journals (Sweden)

Rogério de Almeida Vieira

2004-09-01

Full Text Available Coating of steel surfaces with titanium films has been studied with the objective to protect them against corrosion, and to create an intermediate film for CVD diamond and TiN film deposition. In this work, the nucleation, growth mechanisms and microstructural formation of the titanium films deposited on 304 stainless steel (304 SS substrate are presented and discussed. The titanium films of variable thickness were obtained by vapour phase deposition produced by electron beam. The surfaces of these samples were observed by scanning electron microscopy. The cross sections of these samples were observed by using an atomic force microscope. The Ti film-304 SS interfaces were analyzed by X-ray diffraction. The results showed that titanium films have a columnar growth. The Ti film-304 SS interface had a residual compression stress at room temperature due to the inter-diffusion process.

On fitting the full spectrum of luminous red galaxies by using ULySS and STARLIGHT

International Nuclear Information System (INIS)

Liu Gao-Chao; Lu You-Jun; Chen Xue-Lei; Du Wei; Zhao Yong-Heng

2013-01-01

We select a sample of quiescent luminous red galaxies (LRGs) from the Sloan Digital Sky Survey Data Release 7 with a high signal-to-noise ratio (S/N) to study the consistency of fitting the full spectrum by using different packages, mainly, ULySS and STARLIGHT. The spectrum of each galaxy in the sample is fitted by the full spectrum fitting packages ULySS and STARLIGHT. We find: (1) for spectra with higher S/Ns, the ages of stellar populations obtained from ULySS are slightly older than those from STARLIGHT, and metallicities derived from ULySS are slightly richer than those from STARLIGHT. In general, both packages can give roughly consistent fitting results. (2) For low S/N spectra, it is possible that the fitting by ULySS can become trapped at some local minimum in the parameter space during execution and thus may give unreliable results, but STARLIGHT can still give reliable results. Based on the fitting results of LRGs, we further analyze their star formation history and the relation between their age and velocity dispersion, and find that they agree well with conclusions from previous works

Structure/property (constitutive and dynamic strength/damage characterization of additively manufactured 316L SS

Directory of Open Access Journals (Sweden)

Gray III G.T.

2015-01-01

Full Text Available For additive manufacturing (AM, the certification and qualification paradigm needs to evolve as there exists no “ASTM-type” additive manufacturing certified process or AM-material produced specifications. Accordingly, utilization of AM materials to meet engineering applications requires quantification of the constitutive properties of these evolving materials in comparison to conventionally-manufactured metals and alloys. Cylinders of 316L SS were produced using a LENS MR-7 laser additive manufacturing system from Optomec (Albuquerque, NM equipped with a 1kW Yb-fiber laser. The microstructure of the AM-316L SS is detailed in both the as-built condition and following heat-treatments designed to obtain full recrystallization. The constitutive behavior as a function of strain rate and temperature is presented and compared to that of nominal annealed wrought 316L SS plate. The dynamic damage evolution and failure response of all three materials was probed using flyer-plate impact driven spallation experiments at a peak stress of 4.5 GPa to examine incipient spallation response. The spall strength of AM-produced 316L SS was found to be very similar for the peak shock stress studied to that of annealed wrought or AM-316L SS following recrystallization. The damage evolution as a function of microstructure was characterized using optical metallography.

Clinical significance of changes of serum TGF-β1, CTGF and SS levels in patients with chronic hepatitis C

International Nuclear Information System (INIS)

Liu Chunyan

2011-01-01

Objective: To esplore the clinical significance of serum TGF-β 1 , CTGF and SS levels in patients with chronic hepatitis C. Methods: Serum TGF-β 1 , SS (with RIA) serum CTGF (with ELISA) levels were measured in 38 patients with chronic hepatitis C and 35 normal healthy controls. Results: Serum TGF-β 1 , CTGF and SS levels were remarkably higher than those in controls (P 1 levels were positively correlated with CTGF and SS levels (r=0.6134, 0.4916, P 1 , CTGF and SS levels may help to recognize the pathogenesis and prediction in chronic hepatitis C. (authors)

Oxynitrides decorated 316L SS for potential bioimplant application

Science.gov (United States)

Saravanan Kaliaraj, Gobi; Kumar, N.

2018-03-01

Titanium oxynitride (TiON) and zirconium oxynitride (ZrON) were deposited onto 316L stainless steel (316L SS) using reactive magnetron sputtering technique. The monoclinic and cubic phase of TiON and ZrON were obtained by x-ray diffraction (XRD). Nanoindentation and wear test analysis exhibited the better mechanical properties of TiON and ZrON films. Wettability studies showed hydrophilic nature on coated films; whereas bare 316L SS substrate was least hydrophilic. Drastic reduction of bacterial adhesion (Pseudomonas aeruginosa), as well as biofilm formation, was observed in both the films at different time duration. TiON and ZrON films were exhibited excellent hemocompatibility by preventing the platelet activation. Furthermore, the coated films exhibited corrosion protection in presence and absence of hydrogen peroxide (H2O2) in artificial blood plasma (ABP) solution.

Asiakkaan profilointi matkailupalveluiden puhelinmyyntityössä

OpenAIRE

Laaksonen, Aura; Taralainen, Tuija

2010-01-01

Tämän opinnäytetyön aiheena on asiakkaan profilointi puhelinmyyntityössä. Työn toimeksiantaja on Itä-Suomen yliopiston Matkailualan opetus- ja tutkimuslaitos. Tutkimuksessamme selvitimme videotallenteiden avulla, millä tavoin myyjä profiloi asiakasta puhelinmyynnissä, ja missä myyntiprosessin vaiheessa profilointia tapahtuu. Tutkimuksen tulosten perusteella toimeksiantajan on tarkoitus luoda matkailupalveluyritykselle verkossa toimiva matkanvarausjärjestelmä. Teoriaosassa käsittelimme asi...

ssDNA degradation along capillary electrophoresis process using a Tris buffer.

Science.gov (United States)

Ric, Audrey; Ong-Meang, Varravaddheay; Poinsot, Verena; Martins-Froment, Nathalie; Chauvet, Fabien; Boutonnet, Audrey; Ginot, Frédéric; Ecochard, Vincent; Paquereau, Laurent; Couderc, François

2017-06-01

Tris-Acetate buffer is currently used in the selection and the characterization of ssDNA by capillary electrophoresis (CE). By applying high voltage, the migration of ionic species into the capillary generates a current that induces water electrolysis. This phenomenon is followed by the modification of the pH and the production of Tris derivatives. By injecting ten times by capillary electrophoresis ssDNA (50 nM), the whole oligonucleotide was degraded. In this paper, we will show that the Tris buffer in the running vials is modified along the electrophoretic process by electrochemical reactions. We also observed that the composition of the metal ions changes in the running buffer vials. This phenomenon, never described in CE, is important for fluorescent ssDNA analysis using Tris buffer. The oligonucleotides are degraded by electrochemically synthesized species (present in the running Tris vials) until it disappears, even if the separation buffer in the capillary is clean. To address these issues, we propose to use a sodium phosphate buffer that we demonstrate to be electrochemically inactive. © 2017 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Evaluation of adjuvant activity of fractions derived from Agaricus blazei, when in association with the recombinant LiHyp1 protein, to protect against visceral leishmaniasis.

Science.gov (United States)

de Jesus Pereira, Nathália Cristina; Régis, Wiliam César Bento; Costa, Lourena Emanuele; de Oliveira, Jamil Silvano; da Silva, Alanna Gomes; Martins, Vivian Tamietti; Duarte, Mariana Costa; de Souza, José Roberto Rodrigues; Lage, Paula Sousa; Schneider, Mônica Santos; Melo, Maria Norma; Soto, Manuel; Soares, Sandra Aguiar; Tavares, Carlos Alberto Pereira; Chávez-Fumagalli, Miguel Angel; Coelho, Eduardo Antonio Ferraz

2015-06-01

The development of effective prophylactic strategies to prevent leishmaniasis has become a high priority. No less important than the choice of an antigen, the association of an appropriate adjuvant is necessary to achieve a successful vaccination, as the majority of the tested antigens contain limited immunogenic properties, and need to be supplemented with immune response adjuvants in order to boost their immunogenicity. However, few effective adjuvants that can be used against leishmaniasis exist on the market today; therefore, it is possible to speculate that the research aiming to identify new adjuvants could be considered relevant. Recently, Agaricus blazei extracts have proved to be useful in enhancing the immune response to DNA vaccines against some diseases. This was based on the Th1 adjuvant activity of the polysaccharide-rich fractions from this mushroom. In this context, the present study evaluated purified fractions derived from Agaricus blazei as Th1 adjuvants through in vitro assays of their immune stimulation of spleen cells derived from naive BALB/c mice. Two of the tested six fractions (namely F2 and F4) were characterized as polysaccharide-rich fractions, and were able to induce high levels of IFN-γ, and low levels of IL-4 and IL-10 in the spleen cells. The efficacy of adjuvant action against L. infantum was evaluated in BALB/c mice, with these fractions being administered together with a recombinant antigen, LiHyp1, which was previously evaluated as a vaccine candidate, associated with saponin, against visceral leishmaniasis (VL). The associations between LiHyp1/F2 and LiHyp1/F4 were able to induce an in vivo Th1 response, which was primed by high levels of IFN-γ, IL-12, and GM-CSF, by low levels of IL-4 and IL-10; as well as by a predominance of IgG2a antibodies in the vaccinated animals. After infection, the immune profile was maintained, and the vaccines proved to be effective against L. infantum. The immune stimulatory effects in the

Dibenzotetraaza[14]annulene-adenine conjugate recognizes complementary poly dT among ss-DNA/ss-RNA sequences.

Science.gov (United States)

Radić Stojković, Marijana; Škugor, Marko; Tomić, Sanja; Grabar, Marina; Smrečki, Vilko; Dudek, Łukasz; Grolik, Jarosław; Eilmes, Julita; Piantanida, Ivo

2013-06-28

Among three novel DBTAA derivatives only the DBTAA-propyl-adenine conjugate showed recognition of the consecutive oligo dT sequence by increased affinity and specific induced chirooptical response in comparison to other single stranded RNA and DNA; whereby of particular importance is the up until now unique efficient differentiation between dT and rU. At variance, its close analogue DBTAA-hexyl-adenine did not reveal any selectivity between ss-DNA/RNA pointing out the important role of steric factors (linker length); moreover non-selectivity of the reference compound (, lacking adenine) stressed the importance of adenine interactions in the selectivity.

Aqueous Extract of Agaricus blazei Murrill Prevents Age-Related Changes in the Myenteric Plexus of the Jejunum in Rats

Directory of Open Access Journals (Sweden)

Ana Paula de Santi-Rampazzo

2015-01-01

Full Text Available This study evaluated the effects of the supplementation with aqueous extract of Agaricus blazei Murrill (ABM on biometric and blood parameters and quantitative morphology of the myenteric plexus and jejunal wall in aging Wistar rats. The animals were euthanized at 7 (C7, 12 (C12 and CA12, and 23 months of age (C23 and CA23. The CA12 and CA23 groups received a daily dose of ABM extract (26 mg/animal via gavage, beginning at 7 months of age. A reduction in food intake was observed with aging, with increases in the Lee index, retroperitoneal fat, intestinal length, and levels of total cholesterol and total proteins. Aging led to a reduction of the total wall thickness, mucosa tunic, villus height, crypt depth, and number of goblet cells. In the myenteric plexus, aging quantitatively decreased the population of HuC/D+ neuronal and S100+ glial cells, with maintenance of the nNOS+ nitrergic subpopulation and increase in the cell body area of these populations. Supplementation with the ABM extract preserved the myenteric plexus in old animals, in which no differences were detected in the density and cell body profile of neurons and glial cells in the CA12 and CA23 groups, compared with C7 group. The supplementation with the aqueous extract of ABM efficiently maintained myenteric plexus homeostasis, which positively influenced the physiology and prevented the death of the neurons and glial cells.

Scalable and reusable emulator for evaluating the performance of SS7 networks

Science.gov (United States)

Lazar, Aurel A.; Tseng, Kent H.; Lim, Koon Seng; Choe, Winston

1994-04-01

A scalable and reusable emulator was designed and implemented for studying the behavior of SS7 networks. The emulator design was largely based on public domain software. It was developed on top of an environment supported by PVM, the Parallel Virtual Machine, and managed by OSIMIS-the OSI Management Information Service platform. The emulator runs on top of a commercially available ATM LAN interconnecting engineering workstations. As a case study for evaluating the emulator, the behavior of the Singapore National SS7 Network under fault and unbalanced loading conditions was investigated.

Elokuvan rooli nuorten elämässä ja nuorisotilojen arjessa

OpenAIRE

Mäkinen, Kirsi

2009-01-01

Elokuva on arkipäiväinen taiteenmuoto, jota erityisesti nuoret seuraavat aktiivisesti. Halusinkin opinnäy-tetyössäni selvittää mitä elokuva nuorille oikeastaan merkitsee. Lisäksi minua kiinnosti tutkia työssäni millaisessa asemassa elokuvien esittäminen on nuorisotiloilla. Tutkimuksen alkuvaiheessa selvisi, että mikkeliläisillä nuorisotaloilla ei esitetä elokuvia, koska ei ole tarvittavia lupia. Sen sijaan Tampereella esitetään elokuvia ilman mitään lupia. Näin työstäni syntyi tapaustutkimus,...

Microbiological features and clinical impact of the type VI secretion system (T6SS) in Acinetobacter baumannii isolates causing bacteremia.

Science.gov (United States)

Kim, Jungok; Lee, Ji-Young; Lee, Haejeong; Choi, Ji Young; Kim, Dae Hun; Wi, Yu Mi; Peck, Kyong Ran; Ko, Kwan Soo

2017-10-03

We investigated the genetic background and microbiological features of T6SS-positive Acinetobacter baumannii isolates and clinical impact of the T6SS in patients with A. baumannii bacteremia. One hundred and 62 A. baumannii isolates from patients with bacteremia in 2 tertiary-care hospitals in Korea were included in this study. Approximately one-third (51/162, 31.5%) of the A. baumannii clinical isolates possessed the hcp gene, and the hcp-positive isolates were found in several genotypes in multilocus sequence typing. The expression and secretion of Hcp protein varied among the clinical isolates. A. baumannii isolates with detectable Hcp secretion (T6SS+) could better outcompete Escherichia coli compared with T6SS- isolates, including hcp-negative and inactivated hcp-positive isolates. In addition, T6SS+ isolates showed higher biofilm-forming activity and better survival in the presence of normal human serum than the T6SS- isolates. T6SS+ isolates were more frequently detected in patients with catheter-related bloodstream infection, haematopoietic stem cell transplant recipients, and patients receiving immunosuppressive agents. However, T6SS was not a prognostic factor for mortality. Our results suggest that the T6SS of A. baumannii is associated with virulence and contributes to infections in immunocompromised patients and those with implanted medical devices.

Design and specificity of long ssDNA donors for CRISPR-based knock-in

OpenAIRE

Leonetti, Manuel; Li, Han; Beckman, Kyle; Pessino, Veronica; Huang, Bo; Weissman, Jonathan

2017-01-01

CRISPR/Cas technologies have transformed our ability to manipulate genomes for research and gene-based therapy. In particular, homology-directed repair after genomic cleavage allows for precise modification of genes using exogenous donor sequences as templates. While both single-stranded DNA (ssDNA) and double-stranded DNA (dsDNA) forms of donors have been used as repair templates, a systematic comparison of the performance and specificity of repair using ssDNA versus dsDNA donors is still la...

Echinococcus granulosus sensu stricto (s.s.) from the critically endangered antelope Addax nasomaculatus in Tunisia.

Science.gov (United States)

Boufana, Belgees; Saïd, Yousra; Dhibi, Mokhtar; Craig, Philip S; Lahmar, Samia

2015-12-01

Echinococcus granulosus sensu lato (s.l.) is a zoonotic disease highly endemic in Tunisia. Canids including stray and semi-stray dogs, jackals and foxes are known as definitive hosts and a wide range of ungulates have been shown to harbour the metacestode hydatid stage and may serve as intermediate hosts. Fertile hydatid cysts of Echinococcus equinus and E. granulosus sensu stricto (s.s.) were recently molecularly identified for the first time from Tunisian donkeys. E. granulosus (s.s.) was also identified from wild boars in Tunisia. Here we report the confirmation of hydatid cysts caused by E. granulosus (s.s.) in the critically endangered antelope, Addax nasomaculatus in Tunisia. DNA-based molecular analysis revealed that A.nasomaculatus was infected with E. granulosus (s.s.) which had a 100% identity with the main globally distributed E. granulosus (s.s.) (EgTu01) haplotype. Cysts of Taenia hydatigena (n=33) were also observed on the liver and in the body cavity. Due to their endangered status and their relatively small numbers, it is unlikely that hydatid infection of A. nasomaculatus will form a major contribution to the epidemiology and transmission of E. granulosus in Tunisia, but infection may result in pathology, morbidity and early mortality, and may still play a role in the perpetuation of the parasite in wildlife cycles. Copyright © 2015 Elsevier B.V. All rights reserved.

Tapahtuman mallintaminen kauppakeskusmiljöössä

OpenAIRE

Mäenpää, Piia

2016-01-01

Opinnäytetyön tarkoituksena oli luoda kauppakeskusmiljööseen sopiva tapahtumakonsepti kohdennetulle asiakaskohderyhmälle, tässä tapauksessa seniori-ikäisille yli 65-vuotiaille. Kokonaisvaltaisesti tapahtuman mallinnuksen tavoitteeksi määriteltiin lisäksi uudenlaisen asiakaslähtöisen palvelukulttuurin luominen kauppakeskusmiljööseen, asiakkaiden aktivointi palveluiden sekä synergian luominen yksityisten eri toimijoiden välillä kauppakeskuksessa ja osuuskaupan sisällä. Konseptoinnin tausto...

Hoitajien kokemuksia Theraplay-ryhmäsovelluksesta lastenpsykiatrisessa hoitotyössä

OpenAIRE

Manninen, Niina; Ollikainen, Laura

2011-01-01

Tämän opinnäytetyön tarkoituksena oli selvittää hoitajien kokemuksia ja ajatuksia Theraplay-ryhmäsovelluksesta lastenpsykiatrisessa hoitotyössä sekä kartoittaa hoitajien mielipiteitä mahdollisesta tanssin ja musiikin käytöstä osana tätä sovellusta. Opinnäytetyön tehtävänä oli selvittää millaisia kokemuksia hoitajilla on Theraplay-ryhmäsovelluksesta, mitä ajatuksia mahdollinen musiikin ja tanssin käyttö osana Theraplay-ryhmäsovellusta herättää sekä mitä kehitettävää tämän sovelluksen käytössä ...

Clinical significance of determination of changes of plasma ET and SS contents in neonates with hypoxic-ischemic encephalopathy (HIE)

International Nuclear Information System (INIS)

Zhang Yuhong; Chen Chuanbing; Li Hua

2008-01-01

Objective: To explore the clinical significance of changes of plasma ET and somatostatin (SS) levels in neonates with hypoxic-ischemic encephalopathy (HIE). Methods: Plasma ET and SS contents were determined with RIA in 63 neonates with hypoxic-ischemic encephalopathy and 35 controls. Results: In neonates with HIE, the plasma ET levels were significantly higher than those in the controls (P<0.01), while the plasma SS levels were significantly lower (P<0.01). Conclusion: Development of hypoxic-ischemic encephalopathy in newborn infants was closely associated with increase of plasma ET and SS levels. (authors)

Katariina kirikus algab Geeniuste mäss / Kai Ilustrumm

Index Scriptorium Estoniae

Ilustrumm, Kai

2004-01-01

Briti Nõukogu toetusel Pärnus, Tartus ja Tallinnas toimuvatel inglise filmide päevadel "Geeniuste mäss" näidatakse ka 3 Lindsay Andersoni nn. Travise triloogia filmi "Kui..." (1968), "Oo, õnneseen!" (1973) ja "Ravila Britannia" (1982), kus Mick Travise rollis on Malcolm McDowell (1943). Lisatud kava

Tributyylitina (TBT) maaympäristössä: esiintyminen, vaikutukset ja riskit

OpenAIRE

Lukkari, Tuomas; Koponen, Kari; Tuomi, Pirjo; Dahlbo, Kim; Rossi, Esko; Järvinen, Kimmo

2006-01-01

Organometalleihin kuuluvan tributyylitinan (TBT) esiintymisestä, vaikutuksista ja mahdollisista riskeistä maaympäristössä on vain vähän tutkimustietoa. Tiedot perustuvat pääasiassa vesiympäristöissä tehtyihin tutkimuksiin. TBT:a ei esiinny ympäristössä luonnostaan, vaan se on peräisin ihmisen toiminnasta. TBT:n päästöt maaperään ovat peräisin mm. TBT:a sisältävien valmisteiden käytöstä, lähinnä laivojen pohjamaaleista sekä mahdollisesti pilaantuneiden sedimenttien loppusijoituksesta. TBT:n...

An MC-SS Platform for Short-Range Communications in the Personal Network Context

Directory of Open Access Journals (Sweden)

Bakirtzoglou Zeta

2008-01-01

Full Text Available Abstract Wireless personal area networks (WPANs have gained interest in the last few years, and several air interfaces have been proposed to cover WPAN applications. A multicarrier spread spectrum (MC-SS air interface specified to achieve 130 Mbps in typical WPAN channels is presented in this paper. It operates in the 5.2 GHz ISM band and achieves a spectral efficiency of 3.25  . Besides the robustness of the MC-SS approach, this air interface yields to reasonable implementation complexity. This paper focuses on the hardware design and prototype of this MC-SS air interface. The prototype includes RF, baseband, and IEEE802.15.3 compliant medium access control (MAC features. Implementation aspects are carefully analyzed for each part of the prototype, and key hardware design issues and solutions are presented. Hardware complexity and implementation loss are compared to theoretical expectations, as well as flexibility is discussed. Measurement results are provided for a real condition of operations.

Enormous periodic doppler shifts in SS 433

International Nuclear Information System (INIS)

Margon, B.; Ford, H.C.; Grandi, S.A.; Stone, R.P.S.

1979-01-01

We have previously reported prominent ''moving' emission lines in the visible spectrum of Stephenson-Sanduleak 433, the optical counterpart of a variable radio and X-ray source. Further observations show that despite the implausible velocities and changes in velocities implied if the moving features are interpreted as Doppler-shifted Balmer lines, this explanation is indeed correct. Spectroscopy of SS 433 on 51 mights in 1978--1979 reveals that the unidentified features are two sets of Balmer and He I lines, one with large and changing redshift, and the other with large and changing blueshift. Combining our data with published earlier observations, we obtain Doppler shifts on 80 nights in the period 1978 June to 1979 June. These data indicate that the velocity variations are cyclical, repeating in both the blueshift and redshift systems with a period of 164 +- 3 days. The two systems have thus far been observed to reach maximum positive and negative radial velocities of +50,000 and -35,000 km s -1 , respectively, are always symmetric about redshift z=0.04, and follow roughly sinusoidal velocity curves. We discuss in addition a variety of interesting short-term spectroscopic details, including minor but highly significant deviations of the radial velocity from the sinusoid, and nightly line profile changes, sometimes appearing as mirror-image events in the redshift and blueshift systems. The behavior of SS 433 is unprecedented

Structure of the hDmc1-ssDNA filament reveals the principles of its architecture.

Directory of Open Access Journals (Sweden)

Andrei L Okorokov

Full Text Available In eukaryotes, meiotic recombination is a major source of genetic diversity, but its defects in humans lead to abnormalities such as Down's, Klinefelter's and other syndromes. Human Dmc1 (hDmc1, a RecA/Rad51 homologue, is a recombinase that plays a crucial role in faithful chromosome segregation during meiosis. The initial step of homologous recombination occurs when hDmc1 forms a filament on single-stranded (ss DNA. However the structure of this presynaptic complex filament for hDmc1 remains unknown. To compare hDmc1-ssDNA complexes to those known for the RecA/Rad51 family we have obtained electron microscopy (EM structures of hDmc1-ssDNA nucleoprotein filaments using single particle approach. The EM maps were analysed by docking crystal structures of Dmc1, Rad51, RadA, RecA and DNA. To fully characterise hDmc1-DNA complexes we have analysed their organisation in the presence of Ca2+, Mg2+, ATP, AMP-PNP, ssDNA and dsDNA. The 3D EM structures of the hDmc1-ssDNA filaments allowed us to elucidate the principles of their internal architecture. Similar to the RecA/Rad51 family, hDmc1 forms helical filaments on ssDNA in two states: extended (active and compressed (inactive. However, in contrast to the RecA/Rad51 family, and the recently reported structure of hDmc1-double stranded (ds DNA nucleoprotein filaments, the extended (active state of the hDmc1 filament formed on ssDNA has nine protomers per helical turn, instead of the conventional six, resulting in one protomer covering two nucleotides instead of three. The control reconstruction of the hDmc1-dsDNA filament revealed 6.4 protein subunits per helical turn indicating that the filament organisation varies depending on the DNA templates. Our structural analysis has also revealed that the N-terminal domain of hDmc1 accomplishes its important role in complex formation through domain swapping between adjacent protomers, thus providing a mechanistic basis for coordinated action of hDmc1 protomers

Lead (Pb) bioaccumulation; genera Bacillus isolate S1 and SS19 as a case study

Science.gov (United States)

Arifiyanto, Achmad; Apriyanti, Fitria Dwi; Purwaningsih, Puput; Kalqutny, Septian Hary; Agustina, Dyah; Surtiningsih, Tini; Shovitri, Maya; Zulaika, Enny

2017-06-01

Lead (Pb) includes a group of large heavy metal in nature was toxic either on animal or human and did not provide an advantage function biologically. Bacillus isolates S1 and SS19 known resistant to lead up to 50 mg / L PbCl2. In this research will be examined whether genera Bacillus isolates S1 and SS19 could accumulate metal lead (Pb), their capability in accumulating and profile protein differences when the bacteria genera Bacillus isolates S1 and SS19 get exposed metal lead (Pb). Inoculum at age ± 9 hours are used, with a Nutrient Broth (NB) containing 50, 75 and 100 mg / L PbCl2. Inductively Coupled Plasma Atomic Emission Spectrometry (ICP) used to assessed Pb2+ concentrations. Bioaccumulation levels of Pb2+ by Bacillus isolate S1 and SS19 related to the distinction of beginning concentration to the final concentration. Bacillus isolate S1 achieved 53% and 51% bioaccumulation efficiency rate in lead presence concentration (75 and 100 mg/L) and 51% (50 mg/L). Another way Bacillus isolate SS19 was able to accumulate 57% (50 mg/L PbCl2) and kept stable on 36% bioaccumulation efficiency rate (75 and 100 mg/L PbCl2). Regarding SDS-PAGE electrophoresis protein profile result, protein in ± 127 kDa, molecule mass detected in the presence of Lead for Bacillus isolate S1.

Aliupseereiden työssä oppiminen - Tapaustutkimus sosiomateriaalisten oppimisverkostojen näkökulmasta

OpenAIRE

Pekkarinen, Otto

2017-01-01

Tutkimuksessa tarkasteltiin Puolustusvoimien perusyksiköissä kouluttajatehtävissä toimivien aliupseerien työssä oppimista ja sen ohjaamista. Työssä oppimista tarkasteltiin kulttuurihistori-allisen toiminnan teorian ja tietoa luovan oppimisen metaforan mukaisen trialogisen oppimisen tarjoamilla teoreettisilla työkaluilla. Tutkimuksen teoreettinen näkökulma kiteytettiin sosiomate-riaalisen oppimisverkostoon, joka määriteltiin toiminnan yhteisen kohteen ympärille rakentu-neeksi yksilön, hänen yh...

A second wave of Salmonella T3SS1 activity prolongs the lifespan of infected epithelial cells.

Directory of Open Access Journals (Sweden)

Ciaran E Finn

2017-04-01

Full Text Available Type III secretion system 1 (T3SS1 is used by the enteropathogen Salmonella enterica serovar Typhimurium to establish infection in the gut. Effector proteins translocated by this system across the plasma membrane facilitate invasion of intestinal epithelial cells. One such effector, the inositol phosphatase SopB, contributes to invasion and mediates activation of the pro-survival kinase Akt. Following internalization, some bacteria escape from the Salmonella-containing vacuole into the cytosol and there is evidence suggesting that T3SS1 is expressed in this subpopulation. Here, we investigated the post-invasion role of T3SS1, using SopB as a model effector. In cultured epithelial cells, SopB-dependent Akt phosphorylation was observed at two distinct stages of infection: during and immediately after invasion, and later during peak cytosolic replication. Single cell analysis revealed that cytosolic Salmonella deliver SopB via T3SS1. Although intracellular replication was unaffected in a SopB deletion mutant, cells infected with ΔsopB demonstrated a lack of Akt phosphorylation, earlier time to death, and increased lysis. When SopB expression was induced specifically in cytosolic Salmonella, these effects were restored to levels observed in WT infected cells, indicating that the second wave of SopB protects this infected population against cell death via Akt activation. Thus, T3SS1 has two, temporally distinct roles during epithelial cell colonization. Additionally, we found that delivery of SopB by cytosolic bacteria was translocon-independent, in contrast to canonical effector translocation across eukaryotic membranes, which requires formation of a translocon pore. This mechanism was also observed for another T3SS1 effector, SipA. These findings reveal the functional and mechanistic adaptability of a T3SS that can be harnessed in different microenvironments.

Microstructural studies of suck cast (Zr-SS)-3 and 5 AI alloys for nuclear metallic waste form

International Nuclear Information System (INIS)

Kumar, P.; Das, N.; Sengupta, P.; Arya, A.; Dey, G.K.

2015-01-01

Management of radioactive metallic waste using 'alloy melting route' is currently being investigated. For disposal of Zr and SS base nuclear metallic wastes, Zr-stainless steel (SS) hybrid alloys are being considered as baseline alloys for developing metallic-waste-form (MWF) alloys. In this context Zr-16 wt. %55 has been selected for MWF alloy in our previous study. In present study, to include amorphous phase in this alloy, 3 and 5 wt. % Al has been added in order to improve desirable properties and useful features of MWF and the two alloys have been prepared by suck casting techniques. Microstructure of these alloys have been investigated by optical and electron microscopy which shows occurrence of two different phases, e.g. dark grey and white phases, in (Zr-16 SS)-3 Al and three different phases, e.g. grey, dark grey and white phases in (Zr-16 SS)-5 AI. Electron diffraction and X-ray diffraction (XRD) analyses of these two alloy specimens revealed the occurrence of Zr (Fe, Cr, AI) (dark grey) and Zr 2 (Fe, Cr, AI) (white) phases in (Zr-16 SS)-3 Al whereas, Zr (Fe, Cr, AI) (dark grey), Zr 2 (Fe, Cr, AI) (grey) and Zr 3 (Fe, Cr, AI) (white) phases were found in (Zr-16 SS)-5 AI. In addition, presence of amorphous phase was indicated by XRD analysis that could be confirmed by transmission electron microscopy of these two alloys. (author)

Proposal and Implementation of An IDS for Potential SMS Spam Signaling Messages on SS7

OpenAIRE

Yeboah, Paul Ntim

2016-01-01

Problem Description Reports on spam activities reaching telecom operators reveal flaws in routing for SMS on the Signaling System 7 network. This thesis consists of the following tasks: A comprehensive Study of vulnerabilities on the SS7 network. Proposal and implementation of an intrusion detection system to mitigate signaling for SMS spam on the SS7 network. Evaluation of the proposed detection method.

Xylanase production by a newly isolated Aspergillus niger SS7 in submerged culture.

Science.gov (United States)

Bakri, Yasser; Al-Jazairi, Manal; Al-Kayat, Ghassan

2008-01-01

Xylanase production by a newly isolated Aspergillus niger SS7 was studied in submerged culture. The optimum initial pH for xylanase production was found to be 7.0. Different agricultural and industrial wastes were evaluated for their ability to induce xylanase production by this isolate. The best xylanase production (293.82 IU/ml) was recorded at 3% (w/v) corn cob hulls after 120 h of incubation. The Aspergillus niger SS7 isolate grown in a simple medium, proved to be a promising microorganism for xylanase production.

Ostotoiminnan laadunvarmistus ja toiminnanohjauksen kehittäminen Mantsinen Group Ltd Oy:ssä

OpenAIRE

Hiltunen, Jarmo

2012-01-01

Opinnäytetyön aiheena oli ostotoiminnan laadunvarmistuksen ja toiminnanohjauksen kehittäminen Mantsinen Group Ltd Oy:ssä. Työssä on perehdytty erityisesti niihin ostotoiminnan laadun kehittämisen haasteisiin mitkä liittyvät alihankintaostamisen osa-alueeseen. Opinnäytetyön päätavoitteena oli luoda työkaluja hankinnan laadun jatkuvan parantamisen avuksi. Lisäksi tavoitteena oli perehtyä hankintojen mittaamisen teoriaan. Opinnäytetyön tutkimusmenetelmänä on käytetty käytännön tietoa ostam...

Clinical significance of determination of serum IGF-I, TNF-α and SS levels in patients with chronic viral hepatitis

International Nuclear Information System (INIS)

Zhu Peiming

2009-01-01

Objective: To study the clinical significance of changes of serum IGF-I, TNF-α and somatostatin(SS) levels in patients with chronic viral hepatitis. Methods: Serum IGF-I, TNF-α and SS levels were determined with RIA in 30 patients with chronic viral hepatitis (B hepatitis, n=24,C hepatitis, n=6) and 30 controls.Results The serum IGF-I, TNF-α and SS levels in the patients were all significantly higher than those in controls (P<0.01). Conclusion: Serum IGF-I, TNF-α and SS levels were markedly increased in patients with chronic viral hepatitis, the exact mechanism and consequence of the changes required further study. (authors)

Pseudomonas aeruginosa uses T3SS to inhibit diabetic wound healing.

Science.gov (United States)

Goldufsky, Josef; Wood, Stephen J; Jayaraman, Vijayakumar; Majdobeh, Omar; Chen, Lin; Qin, Shanshan; Zhang, Chunxiang; DiPietro, Luisa A; Shafikhani, Sasha H

2015-01-01

Diabetic foot ulcers are responsible for more hospitalizations than any other complication of diabetes. Bacterial infection is recognized as an important factor associated with impaired healing in diabetic ulcers. Pseudomonas aeruginosa is the most frequently detected Gram-negative pathogen in diabetic ulcers. P. aeruginosa infection has been shown to impair healing in diabetic wounds in a manner that correlates with its ability to form biofilm. While the majority of infections in diabetic ulcers are biofilm associated, 33% of infections are nonbiofilm in nature. P. aeruginosa is the most prevalent Gram-negative pathogen in all diabetic wound types, which suggests that the deleterious impact of P. aeruginosa on healing in diabetic wounds goes beyond its ability to form biofilm and likely involves other factors. The Type III Secretion System (T3SS) virulence structure is required for the pathogenesis of all P. aeruginosa clinical isolates, suggesting that it may also play a role in the inhibition of wound repair in diabetic skin ulcers. We evaluated the role of T3SS in mediating P. aeruginosa-induced tissue damage in the wounds of diabetic mice. Our data demonstrate that P. aeruginosa establishes a robust and persistent infection in diabetic wounds independent of its ability to form biofilm and causes severe wound damage in a manner that primarily depends on its T3SS. © 2015 by the Wound Healing Society.

Sleeping sickness (ss) in the Abraka Belt: a preliminary ...

African Journals Online (AJOL)

Twenty six parasitologically confirmed SS cases by microscopic examination of blood, lymph node aspirate or cerebrospinal fluid (CSF)) made up of 14 (53.8%) males and 12 (46.2%) females aged between 0 – 70years (mean 29.88 +18.89), were seen during the period of study. Peak incidence occurred at age 0 – 20 years ...

A Bio-Electro-Fenton System Employing the Composite FePc/CNT/SS316 Cathode

Directory of Open Access Journals (Sweden)

Yi-Ta Wang

2017-02-01

Full Text Available Bio-electro-Fenton microbial fuel cells generate energy through the decomposition of organic matter by microorganisms. The generated electricity drives a Fenton reaction in a cathode chamber, which can be used for the decolorization of dye wastewater. Most of the previous works added expensive platinum catalyst to improve the electrical property of the system. In this research, aligned carbon nanotubes (CNTs were generated on the surface of SS316 stainless steel by chemical vapor deposition, and an iron phthalocyanine (FePc catalyst was added to fabricate a compound (FePc/CNT/SS316 that was applied to the cathode electrode of the fuel cell system. This was expected to improve the overall electricity generation efficiency and extent of decolorization of the system. The results showed that the maximum current density of the system with the modified electrode was 3206.30 mA/m2, and the maximum power was 726.55 mW/m2, which were increased by 937 and 2594 times, respectively, compared to the current and power densities of a system where only the SS316 stainless steel electrode was used. In addition, the decolorization of RB5 dye reached 84.6% within 12 h. Measurements of the electrical properties of bio-electro-Fenton microbial fuel cells and dye decolorization experiments with the FePc/CNT/SS316 electrode showed good results.

SS-mPEG chemical modification of recombinant phospholipase C for enhanced thermal stability and catalytic efficiency.

Science.gov (United States)

Fang, Xian; Wang, Xueting; Li, Guiling; Zeng, Jun; Li, Jian; Liu, Jingwen

2018-05-01

PEGylation is one of the most promising and extensively studied strategies for improving the properties of proteins as well as enzymic physical and thermal stability. Phospholipase C, hydrolyzing the phospholipids offers tremendous applications in diverse fields. However, the poor thermal stability and higher cost of production have restricted its industrial application. This study focused on improving the stabilization of recombinant PLC by chemical modification with methoxypolyethylene glycol-Succinimidyl Succinate (SS-mPEG, MW 5000). PLC gene from isolate Bacillus cereus HSL3 was fused with SUMO, a novel small ubiquitin-related modifier expression vector and over expressed in Escherichia coli. The soluble fraction of SUMO-PLC reached 80% of the total recombinant protein. The enzyme exhibited maximum catalytic activity at 80 °C and was relatively thermostable at 40-70 °C. It showed extensive substrate specificity pattern and marked activity toward phosphatidylcholine, which made it a typical non-specific PLC for industrial purpose. SS-mPEG-PLC complex exhibited an enhanced thermal stability at 70-80 °C and the catalytic efficiency (K cat /K m ) had increased by 3.03 folds compared with free PLC. CD spectrum of SS-mPEG-PLC indicated a possible enzyme aggregation after chemical modification, which contributed to the higher thermostability of SS-mPEG-PLC. The increase of antiparallel β sheets in secondary structure also made it more stable than parallel β sheets. The presence of SS-mPEG chains on the enzyme molecule surface somewhat changed the binding rate of the substrates, leading to a significant improvement in catalytic efficiency. This study provided an insight into the addition of SS-mPEG for enhancing the industrial applications of phospholipase C at higher temperature. Copyright © 2018 Elsevier B.V. All rights reserved.

Roles of C-Terminal Region of Yeast and Human Rad52 in Rad51-Nucleoprotein Filament Formation and ssDNA Annealing.

Directory of Open Access Journals (Sweden)

Nilesh V Khade

Full Text Available Yeast Rad52 (yRad52 has two important functions at homologous DNA recombination (HR; annealing complementary single-strand DNA (ssDNA molecules and recruiting Rad51 recombinase onto ssDNA (recombination mediator activity. Its human homolog (hRAD52 has a lesser role in HR, and apparently lacks mediator activity. Here we show that yRad52 can load human Rad51 (hRAD51 onto ssDNA complexed with yeast RPA in vitro. This is biochemically equivalent to mediator activity because it depends on the C-terminal Rad51-binding region of yRad52 and on functional Rad52-RPA interaction. It has been reported that the N-terminal two thirds of both yRad52 and hRAD52 is essential for binding to and annealing ssDNA. Although a second DNA binding region has been found in the C-terminal region of yRad52, its role in ssDNA annealing is not clear. In this paper, we also show that the C-terminal region of yRad52, but not of hRAD52, is involved in ssDNA annealing. This suggests that the second DNA binding site is required for the efficient ssDNA annealing by yRad52. We propose an updated model of Rad52-mediated ssDNA annealing.

Functionalization of PVC membrane with ss oligonucleotides for a potentiometric biosensor.

Science.gov (United States)

Shishkanova, T V; Volf, R; Krondak, M; Král, V

2007-05-15

A novel application of a single stranded (ss) oligonucleotide as an active component of polymeric membrane in an ion-selective electrode (ISE) is described. The original oligonucleotides, oligo(dA)(15), modified by cholesterol, triphenylmethyl and hexadecyl derivatives, were immobilized into poly(vinyl chloride) (PVC) membrane using extraction protocol. In parallel, the adsorption protocol was used to immobilize unmodified oligo(dA)(15) on the PVC membrane based on tridodecylmethyammonium chloride (TDDMA(+)Cl(-)). Immobilization of ss oligonucleotide probe through spacer was more effective for the potentiometric detection of the hybridization between complementary oligonucleotides. It was found that cholesterol-oligo(dA)(15) modified membranes were sensitive toward complementary oligo(dT)(15) in the concentration range 2-80 nM at pH 7. An explanation for the detection mechanism is proposed.

Efficient Computation of Sparse Matrix Functions for Large-Scale Electronic Structure Calculations: The CheSS Library.

Science.gov (United States)

Mohr, Stephan; Dawson, William; Wagner, Michael; Caliste, Damien; Nakajima, Takahito; Genovese, Luigi

2017-10-10

We present CheSS, the "Chebyshev Sparse Solvers" library, which has been designed to solve typical problems arising in large-scale electronic structure calculations using localized basis sets. The library is based on a flexible and efficient expansion in terms of Chebyshev polynomials and presently features the calculation of the density matrix, the calculation of matrix powers for arbitrary powers, and the extraction of eigenvalues in a selected interval. CheSS is able to exploit the sparsity of the matrices and scales linearly with respect to the number of nonzero entries, making it well-suited for large-scale calculations. The approach is particularly adapted for setups leading to small spectral widths of the involved matrices and outperforms alternative methods in this regime. By coupling CheSS to the DFT code BigDFT, we show that such a favorable setup is indeed possible in practice. In addition, the approach based on Chebyshev polynomials can be massively parallelized, and CheSS exhibits excellent scaling up to thousands of cores even for relatively small matrix sizes.

PCI-SS: MISO dynamic nonlinear protein secondary structure prediction

Directory of Open Access Journals (Sweden)

Aboul-Magd Mohammed O

2009-07-01

Full Text Available Abstract Background Since the function of a protein is largely dictated by its three dimensional configuration, determining a protein's structure is of fundamental importance to biology. Here we report on a novel approach to determining the one dimensional secondary structure of proteins (distinguishing α-helices, β-strands, and non-regular structures from primary sequence data which makes use of Parallel Cascade Identification (PCI, a powerful technique from the field of nonlinear system identification. Results Using PSI-BLAST divergent evolutionary profiles as input data, dynamic nonlinear systems are built through a black-box approach to model the process of protein folding. Genetic algorithms (GAs are applied in order to optimize the architectural parameters of the PCI models. The three-state prediction problem is broken down into a combination of three binary sub-problems and protein structure classifiers are built using 2 layers of PCI classifiers. Careful construction of the optimization, training, and test datasets ensures that no homology exists between any training and testing data. A detailed comparison between PCI and 9 contemporary methods is provided over a set of 125 new protein chains guaranteed to be dissimilar to all training data. Unlike other secondary structure prediction methods, here a web service is developed to provide both human- and machine-readable interfaces to PCI-based protein secondary structure prediction. This server, called PCI-SS, is available at http://bioinf.sce.carleton.ca/PCISS. In addition to a dynamic PHP-generated web interface for humans, a Simple Object Access Protocol (SOAP interface is added to permit invocation of the PCI-SS service remotely. This machine-readable interface facilitates incorporation of PCI-SS into multi-faceted systems biology analysis pipelines requiring protein secondary structure information, and greatly simplifies high-throughput analyses. XML is used to represent the input

Facebook-yhteisöpalvelun hyödyntäminen yrityksen markkinointiviestinnässä

OpenAIRE

Pajamäki, Ida

2014-01-01

Tämän opinnäytetyön tavoitteena oli selvittää, miksi yritysten olisi kannattavaa olla Facebook-yhteisöpalvelussa ja millaisia keinoja niillä on käytössään Facebook-palvelussa markkinoidessaan. Opinnäytetyössä rakennettiin työn toimeksiantajalle, Mainostoimisto Gurulle, oma Facebook-sivu. Guru on Turun ammattikorkeakoulun sisäinen mainostoimisto, jossa suunnitellaan kaikenlaista markkinointiviestintää opiskelijavoimin. Teoriaosassa käsiteltiin lyhyesti sosiaalista mediaa ja Facebook-palvel...

Effect of Microstructure on the Wear Behavior of Heat Treated SS-304 Stainless Steel

OpenAIRE

S. Kumar

2016-01-01

Sliding wear characteristics of some heat treated SS-304 stainless steel against EN-8 steel in dry condition have been studied in the present experimental work. Samples of SS-304 stainless steel have been heated in a muffle furnace in desired temperature and allowed to dwell for two hours. The heated specimen are then cooled in different media namely inside the furnace, open air, cutting grade oil (grade 44) and water at room temperature to obtain different grades of heat treatment. Microstr...

Predictability of Joint Promotion Examinations in SS2 on Academic ...

African Journals Online (AJOL)

This research studied the predictability of joint SS2 promotion examinations of all the command secondary schools in Nigeria on academic performance of students in Senior School Certificate Examinations. The sample consists of 120 students selected at the Command Secondary School, Abakaliki and Command Day ...

Microstructural evolution in dual-ion irradiated 316SS under various helium injection schedules

International Nuclear Information System (INIS)

Kohyama, A.; Igata, N.; Ayrault, G.; Tokyo Univ.

1984-01-01

Dual-ion irradiated 316 SS samples with various helium injection schedules were studied. The intent of using different schedules was to either approximate the MFR condition, mimic the mixed spectrum reactor condition or mimic the fast reactor condition. The objective of this investigation is to study the influence of these different helium injection schedules on the microstructural development under irradiation. The materials for this study was 316 SS (MFE heat) with three thermomechanical pre-irradiation treatments: solution annealed, solution annealed and aged and 20% cold worked. The cavity nucleation and growth stages were investigated using high resolution TEM. (orig.)

Resistance of superhydrophobic and oleophobic surfaces to varied temperature applications on 316L SS

Science.gov (United States)

Shams, Hamza; Basit, Kanza; Saleem, Sajid; Siddiqui, Bilal A.

316L SS also called Marine Stainless Steel is an important material for structural and marine applications. When superhydrophobic and oleophobic coatings are applied on 316L SS it shows significant resistance to wear and corrosion. This paper aims to validate the coatings manufacturer's information on optimal temperature range and test the viability of coating against multiple oil based cleaning agents. 316L SS was coated with multiple superhydrophic and oleohobic coatings and observed under SEM for validity of adhesion and thickness and then scanned under FFM to validate the tribological information. The samples were then dipped into multiple cleaning agents maintained at the range of operating temperatures specified by the manufacturer. Coating was observed for deterioration over a fixed time intervals through SEM and FFM. A comparison was drawn to validate the most critical cleaning agent and the most critical temperature at which the coating fails to leave the base substrate exposed to the environment.

Generation of a new spectral format, the lifetime synchronous spectrum (LiSS), using phase-resolved fluorescence spectroscopy

International Nuclear Information System (INIS)

Shaver, J.M.; McGown, L.B.

1994-01-01

A new fluorescence spectral format is introduced in which fluorescence lifetime is shown as a function of synchronously scanned wavelength to generate a Lifetime Synchronous Spectrum (LiSS). Lifetimes are determined in the frequency domain with the use of Phase-Resolved Fluorescence Spectroscopy (PRFS) to obtain the phase of the fluorescence signal. Theory and construction of the LiSS are presented and experimental results are shown for solutions of single components and simple binary and ternary mixtures. These results show how the lifetime information in the LiSS augments the steady-state intensity information of a standard synchronous spectrum, providing unique information for identification of components and resolution of overlapping spectral peaks. The LiSS technique takes advantage of noise reduction inherent in the extraction of lifetime from PRFS in addition to standard spectral smoothing techniques. The precision of phase determination through PRFS is found to be comparable to that of direct phase measurements at normal fluorescence intensities and superior for low-intensity signals

Isolation and Functional Characterization of a Phenylalanine Ammonia-Lyase Gene (SsPAL1 from Coleus (Solenostemon scutellarioides (L. Codd

Directory of Open Access Journals (Sweden)

Qinlong Zhu

2015-09-01

Full Text Available Phenylalanine ammonia-lyase (PAL is the first enzyme involved in the phenylpropanoid pathway and plays important roles in the secondary metabolisms, development and defense of plants. To study the molecular function of PAL in anthocyanin synthesis of Coleus (Solenostemon scutellarioides (L. Codd, a Coleus PAL gene designated as SsPAL1 was cloned and characterized using a degenerate oligonucleotide primer PCR and RACE method. The full-length SsPAL1 was 2450 bp in size and consisted of one intron and two exons encoding a polypeptide of 711 amino acids. The deduced SsPAL1 protein showed high identities and structural similarities with other functional plant PAL proteins. A series of putative cis-acting elements involved in transcriptional regulation, light and stress responsiveness were found in the upstream regulatory sequence of SsPAL1. Transcription pattern analysis indicated that SsPAL1 was constitutively expressed in all tissues examined and was enhanced by light and different abiotic factors. The recombinant SsPAL1 protein exhibited high PAL activity, at optimal conditions of 60 °C and pH 8.2. Although the levels of total PAL activity and total anthocyanin concentration have a similar variation trend in different Coleus cultivars, there was no significant correlation between them (r = 0.7529, p > 0.1, suggesting that PAL was not the rate-limiting enzyme for the downstream anthocyanin biosynthetic branch in Coleus. This study enables us to further understand the role of SsPAL1 in the phenylpropanoid (flavonoids, anthocyanins biosynthesis in Coleus at the molecular level.

First insights into the genetic diversity of Echinococcus granulosus sensu stricto (s.s.) in Serbia.

Science.gov (United States)

Debeljak, Zoran; Boufana, Belgees; Interisano, Maria; Vidanovic, Dejan; Kulisic, Zoran; Casulli, Adriano

2016-06-15

Cystic echinococcosis (CE) caused by Echinococcus granulosus sensu stricto (s.s.) is a cosmopolitan zoonotic infection which is endemic in Serbia where it is subject to mandatory reporting. However, information on the incidence of the disease in humans and prevalence of hydatid infection in livestock remains limited. We used sequenced data of the cytochrome c oxidase subunit 1 (cox 1) mitochondrial gene to examine the genetic diversity and population structure of E. granulosus (s.s.) from intermediate hosts from Serbia. We also compared our generated nucleotide sequences with those reported for neighbouring European countries. Echinococcus canadensis was molecularly confirmed from pig and human hydatid isolates. E. granulosus (G1) was confirmed from sheep and cattle hydatid isolates as well as the first molecular confirmation in Serbia of E. granulosus G2 in sheep and E. granulosus G3 in sheep and cattle hydatid isolates. The Serbian E. granulosus (s.s.) parsimony network displayed 2 main haplotypes (SB02 and SB05) which together with the neutrality indices were suggestive of bottleneck and/or balancing selection. Haplotype analysis showed the presence of the common E. granulosus haplotype described from other worldwide regions. Investigation of the pairwise fixation (Fst) index suggested that Serbian populations of E. granulosus (s.s.) from sheep and cattle hosts showed moderate genetic differentiation. Six of the Serbian haplotypes (SB02-SB07) were shared with haplotypes from Bulgaria, Hungary and/or Romania. Further studies using a larger number of hydatid isolates from various locations across Serbia will provide more information on the genetic structure of E. granulosus (s.s.) within this region. Copyright © 2016 Elsevier B.V. All rights reserved.

Assuring SS7 dependability: A robustness characterization of signaling network elements

Science.gov (United States)

Karmarkar, Vikram V.

1994-04-01

Current and evolving telecommunication services will rely on signaling network performance and reliability properties to build competitive call and connection control mechanisms under increasing demands on flexibility without compromising on quality. The dimensions of signaling dependability most often evaluated are the Rate of Call Loss and End-to-End Route Unavailability. A third dimension of dependability that captures the concern about large or catastrophic failures can be termed Network Robustness. This paper is concerned with the dependability aspects of the evolving Signaling System No. 7 (SS7) networks and attempts to strike a balance between the probabilistic and deterministic measures that must be evaluated to accomplish a risk-trend assessment to drive architecture decisions. Starting with high-level network dependability objectives and field experience with SS7 in the U.S., potential areas of growing stringency in network element (NE) dependability are identified to improve against current measures of SS7 network quality, as per-call signaling interactions increase. A sensitivity analysis is presented to highlight the impact due to imperfect coverage of duplex network component or element failures (i.e., correlated failures), to assist in the setting of requirements on NE robustness. A benefit analysis, covering several dimensions of dependability, is used to generate the domain of solutions available to the network architect in terms of network and network element fault tolerance that may be specified to meet the desired signaling quality goals.

Lainsuojattomuudesta sukupuolineutraaliin avioliittoon – seksuaalivähemmistöjen aseman kehitys lainsäädännössä

OpenAIRE

Martikainen, Minna

2016-01-01

Tässä opinnäytetyössä tutkitaan, miten seksuaalivähemmistöjen asema Suomessa on kehittynyt lainsäädännössä varhaismodernista ajasta lähtien. Tutkimuskysymystä lähestytään lainopillisesta, oikeushistoriallisesta ja oikeussosiologisesta näkökulmasta. Opinnäytetyössä huomioidaan lakimuutosten lisäksi näin ollen myös evankelis-luterilaisen kirkon ja muiden yhteiskunnan asenteita muokkaavien tahojen sekä myös lääketieteen näkemykset. Opinnäytetyö on jaettu kolmeen päälukuun, jotka on sijoitett...

Identification of the Vibrio parahaemolyticus type III secretion system 2-associated chaperone VocC for the T3SS2-specific effector VopC.

Science.gov (United States)

Akeda, Yukihiro; Kodama, Toshio; Saito, Kazunobu; Iida, Tetsuya; Oishi, Kazunori; Honda, Takeshi

2011-11-01

The enteropathogen Vibrio parahaemolyticus possesses two sets of type III secretion systems, T3SS1 and T3SS2. Effector proteins secreted by these T3SSs are delivered into host cells, leading to cell death or diarrhea. However, it is not known how specific effectors are secreted through a specific T3SS when both T3SSs are expressed within bacteria. One molecule thought to determine secretion specificity is a T3SS-associated chaperone; however, no T3SS2-specific chaperone has been identified. Therefore, we screened T3SS2 chaperone candidates by a pull-down assay using T3SS2 effectors fused with glutathione-S-transferase. A secretion assay revealed that the newly identified cognate chaperone VocC for the T3SS2-specific effector VopC was required for the efficient secretion of the substrate through T3SS2. Further experiments determined the chaperone-binding domain and the amino-terminal secretion signal of the cognate effector. These findings, in addition to the previously identified T3SS1-specific chaperone, VecA, provide a strategy to clarify the specificity of effector secretion through T3SSs of V. parahaemolyticus. 2011 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved.

Reprint of "Echinococcus granulosus sensu stricto (s.s.) from the critically endangered antelope Addax nasomaculatus in Tunisia".

Science.gov (United States)

Boufana, Belgees; Saïd, Yousra; Dhibi, Mokhtar; Craig, Philip S; Lahmar, Samia

2017-01-01

Echinococcus granulosus sensu lato (s.l.) is a zoonotic disease highly endemic in Tunisia. Canids including stray and semi-stray dogs, jackals and foxes are known as definitive hosts and a wide range of ungulates have been shown to harbour the metacestode hydatid stage and may serve as intermediate hosts. Fertile hydatid cysts of Echinococcus equinus and E. granulosus sensu stricto (s.s.) were recently molecularly identified for the first time from Tunisian donkeys. E. granulosus (s.s.) was also identified from wild boars in Tunisia. Here we report the confirmation of hydatid cysts caused by E. granulosus (s.s.) in the critically endangered antelope, Addax nasomaculatus in Tunisia. DNA-based molecular analysis revealed that A. nasomaculatus was infected with E. granulosus (s.s.) which had a 100% identity with the main globally distributed E. granulosus (s.s.) (EgTu01) haplotype. Cysts of Taenia hydatigena (n=33) were also observed on the liver and in the body cavity. Due to their endangered status and their relatively small numbers, it is unlikely that hydatid infection of A. nasomaculatus will form a major contribution to the epidemiology and transmission of E. granulosus in Tunisia, but infection may result in pathology, morbidity and early mortality, and may still play a role in the perpetuation of the parasite in wildlife cycles. Copyright © 2015 Elsevier B.V. All rights reserved.

Impact of the Arizona NExSS Winter School on Interdisciplinary Knowledge and Attitudes

Science.gov (United States)

Huff, Cierra; Burnam-Fink, Michael; Desch, Steven; Apai, Dániel

2018-01-01

The Nexus for Exoplanet System Science (NExSS) is a NASA-funded research coordination network whose focus is on investigating exoplanet diversity and devising strategies for searching for life on exoplanets. The fields of exoplanets and astrobiology are inherently highly interdisciplinary. Progress in these fields demands that researchers with various scientific backgrounds understand the issues and techniques of allied fields of study, including the tools and approaches used to solve different problems, as well as their limitations.In 2016, the NExSS teams at Arizona State University (ASU) and University of Arizona (UA) hosted 32 graduate students and postdoctoral researchers from various scientific backgrounds for one week at the Arizona NExSS Winter School. To bridge the gaps between fields and promote interdisciplinarity, students participated in lessons, field trips, hands-on activities, and a capstone proposal-writing activity. To assess the impact of the School on knowledge and attitudes about other fields, we administered a pre- and post-School questionnaire designed using the Impact Analysis Method of Davis & Scalice (2015).The results show that all participants gained knowledge at the School, especially in areas outside their primary field of study. The questionnaire revealed interesting differences in attitudes as well. When asked whether the geochemistry of Earth without life is predictable, planetary scientists were more likely than average to say yes, and geologists were more likely than average to say no. Their attitudes had converged after participation in the School. These results demonstrate that the Arizona NExSS Winter School was impactful not just in the knowledge gained, but in the interdisciplinary attitudes of students.

[111In-DOTA]LTT-SS28, a first pansomatostatin radioligand for in vivo targeting of somatostatin receptor-positive tumors.

Science.gov (United States)

Maina, Theodosia; Cescato, Renzo; Waser, Beatrice; Tatsi, Aikaterini; Kaloudi, Aikaterini; Krenning, Eric P; de Jong, Marion; Nock, Berthold A; Reubi, Jean Claude

2014-08-14

Radiolabeled pansomatostatin-like analogues are expected to enhance the diagnostic sensitivity and to expand the clinical indications of currently applied sst2-specific radioligands. In this study, we present the somatostatin mimic [DOTA]LTT-SS28 {[(DOTA)Ser1,Leu8,D-Trp22,Tyr25]SS28} and its 111In radioligand. [DOTA]LTT-SS28 exhibited a pansomatostatin-like profile binding with high affinity to all five hsst1-hsst5 subtypes (IC50 values in the lower nanomolar range). Furthermore, [DOTA]LTT-SS28 behaved as an agonist at hsst2, hsst3, and hsst5, efficiently stimulating internalization of the three receptor subtypes. Radioligand [111In-DOTA]LTT-SS28 showed good stability in the mouse bloodstream. It displayed strong and specific uptake in AR42J tumors 4 h postinjection (9.3±1.6% ID/g vs 0.3±0.0% ID/g during sst2 blockade) in mice. Significant and specific uptake was also observed in HEK293-hsst2-, HEK293-hsst3-, and HEK293-hsst5-expressing tumors (4.43±1.5, 4.88±1.1, and DOTA]LTT-SS28 specifically localizes in sst2-, sst3-, and sst5-expressing xenografts in mice showing promise for multi-sst1-sst5 targeted tumor imaging.

Agaricus blazei Murill Polysaccharides Protect Against Cadmium-Induced Oxidative Stress and Inflammatory Damage in Chicken Spleens.

Science.gov (United States)

Xie, Wanqiu; Lv, Ai; Li, Ruyue; Tang, Zequn; Ma, Dexing; Huang, Xiaodan; Zhang, Ruili; Ge, Ming

2018-07-01

Agaricus blazei Murill polysaccharide (ABP) has exhibited antioxidant and immunoregulatory activity. The aim of this study was to investigate the effect of ABP on cadmium (Cd)-induced antioxidant functions and inflammatory damage in chicken spleens. In this study, groups of 7-day-old chickens were fed with normal saline (0.2 mL single/day), CdCl 2 (140 mg/kg/day), ABP (30 mg/mL, 0.2 mL single/day), and Cd + ABP (140 mg/kg/day + 0.2 mL ABP). Spleens were separated on the 20th, 40th, and 60th day for each group. The Cd contents, expression of melanoma-associated differentiation gene 5 (MDA5) and its downstream signaling molecules (interferon promoter-stimulating factor 1 (IPS-1), transcription factors interferon regulatory factor 3 (IRF3), and nuclear factor kappa-light chain-enhancer of activated B cells (NF-κB)), the content of cytokines (interleukin 1β (IL-1β), interleukin 6 (IL-6), tumor necrosis factor-α (TNF-α) and beta interferon (IFN-β)), protein levels of heat shock proteins (HSPs), levels of malondialdehyde (MDA), activities of glutathione peroxidase (GSH-Px) and superoxide dismutase (SOD), and histopathological changes of spleens were detected on the 20th, 40th, and 60th day. The results showed that ABP significantly reduced the accumulation of Cd in the chicken spleens and reduced the expression of MDA5, IPS-1, IRF-3, and NF-κB; their downstream inflammatory cytokines, IL-1β, IL-6, TNF-α, and IFN-β; and the protein levels of HSPs (HSP60, HSP70, and HSP90) in spleens. The activities of antioxidant enzymes (SOD and GSH-Px) significantly increased, and the level of MDA decreased in the ABP + Cd group. The results indicate that ABP has a protective effect on Cd-induced damage in chicken spleens.

Chronic administration of mitochondrion-targeted peptide SS-31 prevents atherosclerotic development in ApoE knockout mice fed Western diet.

Directory of Open Access Journals (Sweden)

Meng Zhang

Full Text Available Oxidative stress and inflammatory factors are deeply involved in progression of atherosclerosis. Mitochondrion-targeted peptide SS-31, selectively targeting to mitochondrial inner membrane reacting with cardiolipin, has been reported to inhibit ROS generation and mitigate inflammation. The present study was designed to investigate whether SS-31 could suppress the development of atherosclerosis in vivo.Male ApoE-/- mice (8 weeks old fed with Western diet were treated with normal saline or SS-31 (1 mg/kg/d or 3 mg/kg/d through subcutaneous injection for 12 weeks. Oil Red O staining was performed to evaluate area and sizes of the plaques. DHE staining and immunohistochemical staining of 8-OHDG was performed to assess the oxidative stress. The aorta ATP contents were assessed by the ATP bioluminescence assay kit. Immunohistochemical staining of CD68 and α-SMA and Masson's trichrome staining were performed to evaluate the composition of atherosclerotic plaque. Biochemical assays were performed to determine the protein level and activity of superoxide dismutase (SOD. The levels of CD36, LOX-1 and ABCA1 were immunohistochemically and biochemically determined to evaluate the cholesterol transport in aorta and peritoneal macrophages. Inflammatory factors, including ICAM-1, MCP-1, IL-6 and CRP in serum, were detected through ELISA.SS-31 administration reduced the area and sizes of western diet-induced atherosclerotic plaques and changed the composition of the plaques in ApoE-/- mice. Oxidative stress was suppressed, as evidenced by the reduced DHE stain, down-regulated 8-OHDG expression, and increased SOD activity after chronic SS-31 administration. Moreover, systemic inflammation was ameliorated as seen by decreasing serum ICAM-1, MCP-1, and IL-6 levels. Most importantly, SS-31 administration inhibited cholesterol influx by down-regulating expression of CD36 and LOX-1 to prevent lipid accumulation to further suppress the foam cell formation and

Applicability of Al-powder-alloy coating to corrosion barriers of 316SS in liquid lead-bismuth eutectic

International Nuclear Information System (INIS)

Kurata, Yuji; Sato, Hidetomo; Yokota, Hitoshi; Suzuki, Tetsuya

2011-01-01

A new Al-alloy coating method using Al, Ti and Fe powders has been applied to 316SS in order to develop corrosion resistant coating in liquid lead-bismuth eutectic (LBE). The 316SS plates with coating layers of different Al concentrations were exposed to liquid LBE with controlled oxygen concentrations of 10 -6 to 10 -4 mass% at 823 K for 3600 ks. While surface oxidation and grain boundary corrosion accompanied by liquid LBE penetration are observed in 316SS without Al-alloy coating, the Al-alloy coating is effective to protect such severe corrosion attacks in liquid LBE. Although the coating layer containing 2.8 mass% Al does not always keep sufficient corrosion resistance, good corrosion resistance is obtained through the Al-oxide film formed in liquid LBE in the coating layer where the average Al concentration is 4.2 mass%. Cracks are formed in the coating layer containing 17.8 mass% Al during the coating process. The Al-powder-alloy coating applied to 316SS is promising as a corrosion resistant coating method in liquid LBE environment. (author)

GSM SiNYALLERİNİN .IP OMURGASI İİZERiNI>E TAŞINMASI (SS7 OVER IP- NettcSS7

Directory of Open Access Journals (Sweden)

Çağatay Neftali Tülü

2003-08-01

Full Text Available Bu çalışmada günümüzün en popüler nıobilhaberleşme sistemi olan GSM [1 l sisteminin, kendiöğeleri aras1ndaki en temel sinyalleşme sistemi olannumara 7 (SS7 [2 sinyallerinin, günümüzün enyaygın iletim omurgast olan ve tüm dünyadakibilgisayarları birbirine bağlayan IP (3] omurgasıüzerinden taşınabilmesi için gerekli si stemin tasar11n1ve gerçeklenınesi üzerinde çahş1lmtştır. Klasil\\ SS7iletimine göre en büyüllt; faydası çok düşül{ maliyetiolan bu sistem sayesinde, dünyadaki b i r çok GSMşebekesi ara bağlanti harcanıalarıııda büyükmiktarda tasarrufa gideceklerdir. Aralarındakiklasik 64 KB/sn 'lik ara bağlantı yerine, bununüzerinde bir bağlantı hızı ile iki şebeke haberleşmişolacaktır. Bu değişimierin etkisi abonelere fiyatlardadüşüş ve servis kalitesinde yükseliş olarakyansıyacaktır.

QoSS Hierarchical NoC-Based Architecture for MPSoC Dynamic Protection

Directory of Open Access Journals (Sweden)

Johanna Sepulveda

2012-01-01

Full Text Available As electronic systems are pervading our lives, MPSoC (multiprocessor system-on-chip security is becoming an important requirement. MPSoCs are able to support multiple applications on the same chip. The challenge is to provide MPSoC security that makes possible a trustworthy system that meets the performance and security requirements of all the applications. The network-on-chip (NoC can be used to efficiently incorporate security. Our work proposes the implementation of QoSS (quality of security service to overcome present MPSoC vulnerabilities. QoSS is a novel concept for data protection that introduces security as a dimension of QoS. QoSS takes advantage of the NoC wide system visibility and critical role in enabling system operation, exploiting the NoC components to detect and prevent a wide range of attacks. In this paper, we present the implementation of a layered dynamic security NoC architecture that integrates agile and dynamic security firewalls in order to detect attacks based on different security rules. We evaluate the effectiveness of our approach over several MPSoCs scenarios and estimate their impact on the overall performance. We show that our architecture can perform a fast detection of a wide range of attacks and a fast configuration of different security policies for several MPSoC applications.

Production of $\\eta$ mesons in 200 AGeV S+S and S+Au reactions

CERN Document Server

Albrecht, R.; Awes, T.C.; Barlag, C.; Berger, F.; Bloomer, M.; Blume, C.; Bock, D.; Bock, R.; Bohne, E.M.; Bucher, D.; Claussen, A.; Clewing, G.; Debbe, R.; Dragon, L.; Eklund, A.; Fokin, S.; Garpman, S.; Glasow, R.; Gustafsson, H.A.; Gutbrod, H.H.; Hansen, O.; Holker, G.; Idh, J.; Ippolitov, M.; Jacobs, P.; Kampert, K.H.; Karadev, K.; Kolb, B.W.; Lebedev, A.; Lohner, H.; Lund, I.; Manko, V.; Moskowitz, B.; Nikolaev, S.; Obenshain, F.E.; Oskarsson, A.; Otterlund, I.; Peitzmann, T.; Plasil, F.; Poskanzer, Arthur M.; Purschke, M.; Roters, B.; Santo, R.; Schmidt, H.R.; Soderstrom, K.; Sorensen, S.P.; Stankus, P.; Steffens, K.; Steinhauser, P.; Stenlund, E.; Stuken, D.; Vinogradov, A.; Wegner, H.E.; Young, G.R.

1995-01-01

Minimum Bias production cross sections of $\\eta$ mesons have been measured in 200AGeV S+Au and S+S collisions at the CERN SPS by reconstructing the $\\eta\\rightarrow\\gamma\\gamma$ decay. The measurements have been made over the rapidity range $2.1 \\leq y \\leq 2.9$ using the leadglass spectrometer of WA80. Within the statistical and systematical uncertainties the spectral shapes of $\\pi~0$ and $\\eta$ mesons yields are identical when their invariant differential cross section is plotted as a function of the transverse mass. The relative normalization of the $\\eta$ to $\\pi~0$ transverse mass spectra is found to be $0.53 \\pm 0.07$ for S+Au and $0.43 \\pm 0.15$ for S+S reactions. Extrapolation to full phase space leads to an integrated cross section ratio of $\\eta$ to $\\pi~0$ mesons of $0.15 \\pm 0.02 {\\rm (stat.)} \\pm 0.02 {\\rm (syst.)}$, and $0.12 \\pm 0.03 {\\rm (stat.)} \\pm 0.02 {\\rm (syst.)}$ for S+Au and S+S collisions, respectively.

Energy Efficiency Experiments on Samsung Exynos 5 Heterogeneous Multicore using OmpSs Task Based Programming

OpenAIRE

Holmgren, Rune

2015-01-01

This thesis explore the energy efficiency of task based programming with OpenMP SuperScalar (OmpSs) on the heterogeneous Samsung Exynos 5422 system on a chip. The system features small energy efficient cores, large high performance cores and a GPGPU, and OmpSs tasks were run on all three different processors. Experiments running a genetic algorithm and a Cholesky decomposition were used to gather results. The option of running applications on the energy efficient cores, on the high perfo...

On the Nature of the Compact Object in SS 433. Observational Evidence of X-Ray Photon Index Saturation

Science.gov (United States)

Seifina, Elena; Titarchuk, Lev

2010-01-01

We present an analysis of the X-ray spectral properties observed from black hole , candidate (BHC) binary SS 433. We have analyzed Rossi X-ray Time Explorer (RXTE) data from this source, coordinated with Green Bank Interferometer/RATAN-600. We show that SS 433 undergoes a X-ray spectral transition from the low hard state (LHS) to the intermediate state (IS). We show that the X-ray broad-band energy spectra during all spectral states are well fit by a sum of so called "Bulk Motion Comptonization (BMC) component" and by two (broad and narrow) Gaussians for the continuum and line emissions respectively. In addition to these spectral model components we also find a strong feature that we identify as a" blackbody-like (BB)" component which color temperature is in the range of 4-5 keV in 24 IS spectra during the radio outburst decay in SS 433. Our observational results on the "high temperature BB" bump leads us to suggest the presence of gravitationally redshifted annihilation line emission in this source. In fact this spectral feature has been recently reproduced in Monte Carlo simulations by Laurent and Titarchuk. We have also established the photon index saturation at about 2.3 in index vs mass accretion correlation. This index-mass accretion correlation allows us to evaluate the low limit of black hole (BH) mass of compact object in SS 433, M(sub bh) approximately > 2 solar masses, using the scaling method using BHC GX 339-4 as a reference source. Our estimate of the BH mass in SS 433 is consistent with recent BH mass measurement using the radial-velocity measurements of the binary system by Hillwig & Gies who find that M(sub x)( = (4.3 +/- 0.8) solar masses. This is the smallest BH mass found up to now among all BH sources. Moreover, the index saturation effect versus mass accretion rate revealed in SS 433, like in a number of other BH candidates, is the strong observational evidence for the presence of a BH in SS 433.

Development in corrosion resistance by microstructural refinement in Zr-16 SS 304 alloy using suction casting technique

Energy Technology Data Exchange (ETDEWEB)

Das, N., E-mail: nirupamd@barc.gov.in; Sengupta, P.; Abraham, G.; Arya, A.; Kain, V.; Dey, G.K.

2016-08-15

Highlights: • Grain refinement was made in Zr–16 wt.% SS alloy while prepared by suction casting process. • Distribution of Laves phase, e.g., Zr{sub 2}(Fe, Cr) was raised in suction cast (SC) Zr–16 wt.% SS. • Corrosion resistance was improved in SC alloy compared to that of arc-melt-cast alloy. • Grain refinement in SC alloy assisted for an increase in its corrosion resistance. - Abstract: Zirconium (Zr)-stainless steel (SS) hybrid alloys are being considered as baseline alloys for developing metallic-waste-form (MWF) with the motivation of disposing of Zr and SS base nuclear metallic wastes. Zr–16 wt.% SS, a MWF alloy optimized from previous studies, exhibit significant grain refinement and changes in phase assemblages (soft phase: Zr{sub 2}(Fe, Cr)/α-Zr vs. hard phase: Zr{sub 3}(Fe, Ni)) when prepared by suction casting (SC) technique in comparison to arc-cast-melt (AMC) route. Variation in Cr-distribution among different phases are found to be low in suction cast alloy, which along with grain refinement restricted Cr-depletion at the Zr{sub 2}(Fe, Cr)/Zr interfaces, prone to localized attack. Hence, SC alloy, compared to AMC alloy, showed lower current density, higher potential at the breakdown of passivity and higher corrosion potential during polarization experiments (carried out under possible geological repository environments, viz., pH 8, 5 and 1) indicating its superior corrosion resistance.

Functional studies of ssDNA binding ability of MarR family protein TcaR from Staphylococcus epidermidis.

Directory of Open Access Journals (Sweden)

Yu-Ming Chang

Full Text Available The negative transcription regulator of the ica locus, TcaR, regulates proteins involved in the biosynthesis of poly-N-acetylglucosamine (PNAG. Absence of TcaR increases PNAG production and promotes biofilm formation in Staphylococci. Previously, the 3D structure of TcaR in its apo form and its complex structure with several antibiotics have been analyzed. However, the detailed mechanism of multiple antibiotic resistance regulator (MarR family proteins such as TcaR is unclear and only restricted on the binding ability of double-strand DNA (dsDNA. Here we show by electrophoretic mobility shift assay (EMSA, electron microscopy (EM, circular dichroism (CD, and Biacore analysis that TcaR can interact strongly with single-stranded DNA (ssDNA, thereby identifying a new role in MarR family proteins. Moreover, we show that TcaR preferentially binds 33-mer ssDNA over double-stranded DNA and inhibits viral ssDNA replication. In contrast, such ssDNA binding properties were not observed for other MarR family protein and TetR family protein, suggesting that the results from our studies are not an artifact due to simple charge interactions between TcaR and ssDNA. Overall, these results suggest a novel role for TcaR in regulation of DNA replication. We anticipate that the results of this work will extend our understanding of MarR family protein and broaden the development of new therapeutic strategies for Staphylococci.

Development of a sensitive electrochemical DNA sensor by 4-aminothiophenol self-assembled on electrodeposited nanogold electrode coupled with Au nanoparticles labeled reporter ssDNA

International Nuclear Information System (INIS)

Li Guangjiu; Liu Lihua; Qi Xiaowei; Guo Yaqing; Sun Wei; Li Xiaolin

2012-01-01

Graphical abstract: - Abstract: A novel and sensitive electrochemical DNA biosensor was fabricated by using the 4-aminothiophenol (4-ATP) self-assembled on electrodeposited gold nanoparticles (NG) modified electrode to anchor capture ssDNA sequences and Au nanoparticles (AuNPs) labeled with reporter ssDNA sequences, which were further coupled with electroactive indicator of hexaammineruthenium (III) ([Ru(NH 3 ) 6 ] 3+ ) to amplify the electrochemical signal of hybridization reaction. Different modified electrodes were prepared and characterized by cyclic voltammetry, scanning electron microscope and electrochemical impedance spectroscopy. By using a sandwich model for the capture of target ssDNA sequences, which was based on the shorter probe ssDNA and AuNPs label reporter ssDNA hybridized with longer target ssDNA, the electrochemical behavior of [Ru(NH 3 ) 6 ] 3+ was monitored by differential pulse voltammetry (DPV). The fabricated electrochemical DNA sensor exhibited good distinguish capacity for the complementary ssDNA sequence and two bases mismatched ssDNA. The dynamic detection range of the target ssDNA sequences was from 1.4 × 10 −11 to 2.0 × 10 −9 mol/L with the detection limit as 9.5 × 10 −12 mol/L (3σ). So in this paper a new electrochemical DNA sensor was designed with gold nanoparticles as the immobilization platform and the signal amplifier simultaneously.

Brucella Rough Mutant Induce Macrophage Death via Activating IRE1α Pathway of Endoplasmic Reticulum Stress by Enhanced T4SS Secretion.

Science.gov (United States)

Li, Peng; Tian, Mingxing; Bao, Yanqing; Hu, Hai; Liu, Jiameng; Yin, Yi; Ding, Chan; Wang, Shaohui; Yu, Shengqing

2017-01-01

Brucella is a Gram-negative facultative intracellular pathogen that causes the worldwide zoonosis, known as brucellosis. Brucella virulence relies mostly on its ability to invade and replicate within phagocytic cells. The type IV secretion system (T4SS) and lipopolysaccharide are two major Brucella virulence factors. Brucella rough mutants reportedly induce the death of infected macrophages, which is T4SS dependent. However, the underlying molecular mechanism remains unclear. In this study, the T4SS secretion capacities of Brucella rough mutant and its smooth wild-type strain were comparatively investigated, by constructing the firefly luciferase fused T4SS effector, BPE123 and VceC. In addition, quantitative real-time PCR and western blotting were used to analyze the T4SS expression. The results showed that T4SS expression and secretion were enhanced significantly in the Brucella rough mutant. We also found that the activity of the T4SS virB operon promoter was notably increased in the Brucella rough mutant, which depends on quorum sensing-related regulators of VjbR upregulation. Cell infection and cell death assays revealed that deletion of vjbR in the Brucella rough mutant absolutely abolished cytotoxicity within macrophages by downregulating T4SS expression. This suggests that up-regulation of T4SS promoted by VjbR in rough mutant Δ rfbE contribute to macrophage death. In addition, we found that the Brucella rough mutant induce macrophage death via activating IRE1α pathway of endoplasmic reticulum stress. Taken together, our study provide evidence that in comparison to the Brucella smooth wild-type strain, VjbR upregulation in the Brucella rough mutant increases transcription of the virB operon, resulting in overexpression of the T4SS gene, accompanied by the over-secretion of effecter proteins, thereby causing the death of infected macrophages via activating IRE1α pathway of endoplasmic reticulum stress, suggesting novel insights into the molecular

Brucella Rough Mutant Induce Macrophage Death via Activating IRE1α Pathway of Endoplasmic Reticulum Stress by Enhanced T4SS Secretion

Directory of Open Access Journals (Sweden)

Peng Li

2017-09-01

Full Text Available Brucella is a Gram-negative facultative intracellular pathogen that causes the worldwide zoonosis, known as brucellosis. Brucella virulence relies mostly on its ability to invade and replicate within phagocytic cells. The type IV secretion system (T4SS and lipopolysaccharide are two major Brucella virulence factors. Brucella rough mutants reportedly induce the death of infected macrophages, which is T4SS dependent. However, the underlying molecular mechanism remains unclear. In this study, the T4SS secretion capacities of Brucella rough mutant and its smooth wild-type strain were comparatively investigated, by constructing the firefly luciferase fused T4SS effector, BPE123 and VceC. In addition, quantitative real-time PCR and western blotting were used to analyze the T4SS expression. The results showed that T4SS expression and secretion were enhanced significantly in the Brucella rough mutant. We also found that the activity of the T4SS virB operon promoter was notably increased in the Brucella rough mutant, which depends on quorum sensing-related regulators of VjbR upregulation. Cell infection and cell death assays revealed that deletion of vjbR in the Brucella rough mutant absolutely abolished cytotoxicity within macrophages by downregulating T4SS expression. This suggests that up-regulation of T4SS promoted by VjbR in rough mutant ΔrfbE contribute to macrophage death. In addition, we found that the Brucella rough mutant induce macrophage death via activating IRE1α pathway of endoplasmic reticulum stress. Taken together, our study provide evidence that in comparison to the Brucella smooth wild-type strain, VjbR upregulation in the Brucella rough mutant increases transcription of the virB operon, resulting in overexpression of the T4SS gene, accompanied by the over-secretion of effecter proteins, thereby causing the death of infected macrophages via activating IRE1α pathway of endoplasmic reticulum stress, suggesting novel insights into the

Effects of a new antiarrhythmic drug SS-68 on electrical activity in working atrial and ventricular myocardium of mouse and their ionic mechanisms

Directory of Open Access Journals (Sweden)

Saida K. Bogus

2015-08-01

Full Text Available SS-68 is a derivative of indole, which demonstrated strong antiarrhythmic effects not associated with significant QT prolongation in dog models of atrial fibrillation. Therefore, SS-68 was proposed as a new antiarrhythmic drug and the present study is the first describing its effects on action potentials (APs configuration and elucidating the ionic mechanisms of these effects. Sharp microelectrodes were used to record APs in isolated preparations of mouse atrial and ventricular myocardium. In both types of myocardium 10−6 M SS-68 produced reduction of AP duration, 3 × 10−6 M failed to alter AP waveform and 10−5 – 3 × 10−5 M prolonged APs. Sensitivity of main ionic currents to SS-68 was determined using whole-cell patch clamp. Transient potassium current Ito was slightly inhibited by SS-68 with IC50 = 1.43 × 10−4 M. IKur was more sensitive with IC50 = 1.84 × 10−5 M. Background inward rectifier showed very low sensitivity to SS-68 – only 10−4 M SS-68 caused significant reduction of IK1. ICaL was significantly inhibited by 10−6M – 3 × 10−5 M SS-68. The IC50 value for the ICaL was 1.84 × 10−6 M. Thus, main ionic currents of mouse cardiomyocytes are inhibited by SS-68 in the following order of potency: ICaL > IKur > Ito > IK1. While lower concentration of SS-68 shorten APs via suppression of ICaL, higher concentrations inhibit K+-currents leading to APs prolongation.

Simulation of tensile stress-strain properties of irradiated type 316 SS by heavily cold-worked material

International Nuclear Information System (INIS)

Muto, Yasushi; Jitsukawa, Shiro; Hishinuma, Akimichi

1995-07-01

Type 316 stainless steel is one of the most promising candidate materials to be used for the structural parts of plasma facing components in the nuclear fusion reactor. The neutron irradiation make the material brittle and reduces its uniform elongation to almost zero at heavy doses. In order to apply such a material of reduced ductility to structural components, the structural integrity should be examined and assured by the fracture mechanics. The procedure requires a formulated stress-strain relationship. However, the available irradiated tensile test data are very limited at present, so that the cold-worked material was used as a simulated material in this study. Property changes of 316 SS, that is, a reduction of uniform elongation and an enhancement of yield stress are seemingly very similar for both the irradiated 316 SS and the cold-worked one. The specimens made of annealed 316 SS, 20% (or 15%) cold worked one and 40% cold worked one were prepared. After the formulation of stress strain behavior, the equation for the cold-worked 316 SS was fitted to the data on irradiated material under the assumption that the yield stress is the same for both materials. In addition, the upper limit for the plastic strain was introduced using the data on the irradiated material. (author)

Electrochemical and in vitro bioactivity of polypyrrole/ceramic nanocomposite coatings on 316L SS bio-implants.

Science.gov (United States)

Madhan Kumar, A; Nagarajan, S; Ramakrishna, Suresh; Sudhagar, P; Kang, Yong Soo; Kim, Hyongbum; Gasem, Zuhair M; Rajendran, N

2014-10-01

The present investigation describes the versatile fabrication and characterization of a novel composite coating that consists of polypyrrole (PPy) and Nb2O5 nanoparticles. Integration of the two materials is achieved by electrochemical deposition on 316L stainless steel (SS) from an aqueous solution of oxalic acid containing pyrrole and Nb2O5 nanoparticles. Fourier transform infrared spectral (FTIR) and X-ray diffraction (XRD) studies revealed that the existence of Nb2O5 nanoparticles in PPy matrix with hexagonal structure. Surface morphological analysis showed that the presence of Nb2O5 nanoparticles strongly influenced the surface nature of the nanocomposite coated 316L SS. Micro hardness results revealed the enhanced mechanical properties of PPy nanocomposite coated 316L SS due to the addition of Nb2O5 nanoparticles. The electrochemical studies were carried out using cyclic polarization and electrochemical impedance spectroscopy (EIS) measurements. In order to evaluate the biocompatibility, contact angle measurements and in vitro characterization were performed in simulated body fluid (SBF) and on MG63 osteoblast cells. The results showed that the nanocomposite coatings exhibit superior biocompatibility and enhanced corrosion protection performance over 316L SS than pure PPy coatings. Copyright © 2014 Elsevier B.V. All rights reserved.

Differential roles of SS18-SSX fusion gene and insulin-like growth factor-1 receptor in synovial sarcoma cell growth

International Nuclear Information System (INIS)

Toernkvist, Maria; Natalishvili, Natalia; Xie Yuntao; Girnita, Ada; D'Arcy, Padraig; Brodin, Bertha; Axelson, Magnus; Girnita, Leonard

2008-01-01

Recently we demonstrated that the synovial sarcoma specific fusion gene SS18-SSX is crucial for cyclin D1 expression and is linked to cell proliferation. In this report we explore the role of SS18-SSX and IGF-1R for their potential functions in cellular proliferation and survival in cultured synovial sarcoma cells. We found that targeting of SS18-SSX mRNA by antisense oligonucleotide treatment drastically and rapidly decreased cell proliferation but caused only a slight increase of apoptosis. The synovial sarcoma cells were confirmed to express IGF-1R, and treatment with an IGF-1R inhibitor resulted in substantially reduced cell viability by inducing apoptosis in these cells. Conversely, inhibition of the IGF-1R resulted only in a slight to moderate decrease in DNA synthesis. In conclusion, SS18-SSX and IGF-1R seem to play important but different roles in maintaining malignant growth of synovial sarcoma cells. Whereas SS18-SSX maintains cyclin D1 and cell proliferation, IGF-1R protects from apoptosis

The Effects of Hot Bending on the Low Cycle Fatigue Behaviors of 347 SS in PWR Primary Environment

Energy Technology Data Exchange (ETDEWEB)

Kim, Ho-Sub; Hong, Jong-Dae; Lee, Junho; Jang, Changheui [Korea Advanced Institute of Science and Technology, Daejeon (Korea, Republic of)

2014-10-15

Fatigue damage could be significant for some locations, especially the welds and bends where stress concentration is typically high. As a possible solution, a large radius hot-bending method has been suggested to eliminate some weld joints and all tight bends. However, for the hot-bending process which involves a high temperature thermal cycle, there is a concern about changes in mechanical properties including low cycle fatigue behaviors. In APR1400, Type 347 SS have been used as surge line pipes. Therefore, to verify the applicability of hot-bending on 347 SS surge line pipes, an environmental fatigue test program was initiated. In this paper, the preliminary results of the on-going test program are introduced. Also, the low cycle fatigue behaviors of 347 SS are compared with those of other grade of stainless steels. The effects of hot bending on the low cycle fatigue behavior of 347 SS were quantitatively evaluated. The fatigue life was compared with the estimated values per NUREG 6909 rev. 1. There are no distinct differences between NUREG 6909 and LCF tests. According to fractography and cross section analysis in progress, basically, the reduction of LCF life of 347 SS in PWR water was caused by operation of HIC mechanism. The cyclic stress responses shows that there is no secondary hardening in 330 .deg.C air and PWR water.

Social class and survival on the S.S. Titanic.

Science.gov (United States)

Hall, W

1986-01-01

Passengers' chances of surviving the sinking of the S.S. Titanic were related to their sex and their social class: females were more likely to survive than males, and the chances of survival declined with social class as measured by the class in which the passenger travelled. The probable reasons for these differences in rates of survival are discussed as are the reasons accepted by the Mersey Committee of Inquiry into the sinking.

EMPReSS: European mouse phenotyping resource for standardized screens.

Science.gov (United States)

Green, Eain C J; Gkoutos, Georgios V; Lad, Heena V; Blake, Andrew; Weekes, Joseph; Hancock, John M

2005-06-15

Standardized phenotyping protocols are essential for the characterization of phenotypes so that results are comparable between different laboratories and phenotypic data can be related to ontological descriptions in an automated manner. We describe a web-based resource for the visualization, searching and downloading of standard operating procedures and other documents, the European Mouse Phenotyping Resource for Standardized Screens-EMPReSS. Direct access: http://www.empress.har.mrc.ac.uk e.green@har.mrc.ac.uk.

Stanford SsTO Mission to Mars: A Realistic, Safe and Cost Effective Approach to Human Mars Exploration Using the Stanford SsTO Launch System

Science.gov (United States)

Osborne, Robert D.

1999-06-01

In recent years, a lot of time and energy has been spent exploring possible mission scenarios for a human mission to Mars. NASA along with the privately funded Mars Society and a number of universities have come up with many options that could place people on the surface of Mars in a relatively short period of time at a relatively low cost. However, a common theme among all or at least most of these missions is that they require heavy lift vehicles such as the Russian Energia or the NASA proposed Magnum 100MT class vehicle to transport large payloads from the surface of Earth into a staging orbit about Earth. However, there is no current budget or any signs for a future budget to review the Russian Energia, the US made Saturn V, or to design and build a new heavy lift vehicle. However, there is a lot of interest and many companies looking into the possibility of "space planes". These vehicles will have the capability to place a payload into orbit without throwing any parts of the vehicle away. The concept of a space plane is basically that the plane is transported to a given altitude either by it's own power or on the back of another air worthy vehicle before the rocket engines are ignited. From this altitude, a Single Step to Orbit (SsTO) vehicle with a significant payload is possible. This report looks at the possibility of removing the requirement of a heavy lift vehicle by using the Stanford designed Single Step to Orbit.(SsTO) Launch Vehicle. The SsTO would eliminate the need for heavy lift vehicles and actually reduce the cost of the mission because of the very low costs involved with each SSTO launch. Although this scenario may add a small amount of risk assembling transfer vehicles in Earth orbit, it should add no additional risk to the crew.

Bioconversion of glycerol for bioethanol production using isolated Escherichia coli SS1

Directory of Open Access Journals (Sweden)

Sheril Norliana Suhaimi

2012-06-01

Full Text Available Bioconverting glycerol into various valuable products is one of glycerol's promising applications due to its high availability at low cost and the existence of many glycerol-utilizing microorganisms. Bioethanol and biohydrogen, which are types of renewable fuels, are two examples of bioconverted products. The objectives of this study were to evaluate ethanol production from different media by local microorganism isolates and compare the ethanol fermentation profile of the selected strains to use of glucose or glycerol as sole carbon sources. The ethanol fermentations by six isolates were evaluated after a preliminary screening process. Strain named SS1 produced the highest ethanol yield of 1.0 mol: 1.0 mol glycerol and was identified as Escherichia coli SS1 Also, this isolated strain showed a higher affinity to glycerol than glucose for bioethanol production.

Isolation and characterization of Halomonas sp. strain C2SS100, a hydrocarbon-degrading bacterium under hypersaline conditions.

Science.gov (United States)

Mnif, S; Chamkha, M; Sayadi, S

2009-09-01

To isolate and characterize an efficient hydrocarbon-degrading bacterium under hypersaline conditions, from a Tunisian off-shore oil field. Production water collected from 'Sercina' petroleum reservoir, located near the Kerkennah island, Tunisia, was used for the screening of halotolerant or halophilic bacteria able to degrade crude oil. Bacterial strain C2SS100 was isolated after enrichment on crude oil, in the presence of 100 g l(-1) NaCl and at 37 degrees C. This strain was aerobic, Gram-negative, rod-shaped, motile, oxidase + and catalase +. Phenotypic characters and phylogenetic analysis based on the 16S rRNA gene of the isolate C2SS100 showed that it was related to members of the Halomonas genus. The degradation of several compounds present in crude oil was confirmed by GC-MS analysis. The use of refined petroleum products such as diesel fuel and lubricating oil as sole carbon source, under the same conditions of temperature and salinity, showed that significant amounts of these heterogenic compounds could be degraded. Strain C2SS100 was able to degrade hexadecane (C16). During growth on hexadecane, cells surface hydrophobicity and emulsifying activity increased indicating the production of biosurfactant by strain C2SS100. A halotolerant bacterial strain Halomonas sp. C2SS100 was isolated from production water of an oil field, after enrichment on crude oil. This strain is able to degrade hydrocarbons efficiently. The mode of hydrocarbon uptake is realized by the production of a biosurfactant which enhances the solubility of hydrocarbons and renders them more accessible for biodegradation. The biodegradation potential of the Halomonas sp. strain C2SS100 gives it an advantage for possibly application on bioremediation of water, hydrocarbon-contaminated sites under high-salinity level.

The Masses and Evolutionary State of the Stars in the Dwarf Nova SS Cygni

Science.gov (United States)

Bitner, Martin A.; Robinson, Edward L.; Behr, Bradford B.

2007-06-01

The dwarf nova SS Cygni is a close binary star consisting of a K star transferring mass to a white dwarf by way of an accretion disk. We have obtained new spectroscopic observations of SS Cyg. Fits of synthetic spectra for Roche lobe-filling stars to the absorption-line spectrum of the K star yield the amplitude of the K star's radial velocity curve and the mass ratio, KK=162.5+/-1.0 km s-1 and q=MK/MWD=0.685+/-0.015. The fits also show that the accretion disk and white dwarf contribute a fraction f=0.535+/-0.075 of the total flux at 5500 Å. Taking the weighted average of our results with previously published results obtained using similar techniques, we find =163.7+/-0.7 km s-1 and =0.683+/-0.012. The orbital light curve of SS Cyg shows an ellipsoidal variation diluted by light from the disk and white dwarf. From an analysis of the ellipsoidal variations, we limit the orbital inclination to the range 45degAustin, the Pennsylvania State University, Stanford University, Ludwig-Maximilians-Universität München, and Georg-August-Universität Göttingen.

Pala-animaatiohahmojen animointi 2D- ja 3D-ympäristössä

OpenAIRE

Koivisto, Ida

2011-01-01

Opinnäytetyö käsittelee hahmoanimointia digitaalisessa pala-animaatiossa. Työssä keskitytään pääasiassa vertailemaan keskenään kahta hyvin erityyppistä ohjelmaa hahmoanimaattorin työskentely-ympäristöinä. Ohjelmat, joita opinnäytetyössä on käytetty animoimiseen, ovat 3D-ohjelma Cinema 4D sekä 2D-2.5D -ohjelma After Effects. Opinnäytetyö on kirjoitettu hahmoanimaattorin näkökulmasta perustuen käytännön animointityöskentelyyn lasten animaatiosarjassa Ella & Aleksi. Työn tekijä on toiminut p...

Significant Variation of Post-critical SsPmp Amplitude as a Result of Variation in Near-surface Velocity: Observations from the Yellowknife Array

Science.gov (United States)

Ferragut, G.; Liu, T.; Klemperer, S. L.

2017-12-01

In recent years Virtual Deep Seismic Sounding (VDSS) emerged as a novel method to image the Moho, which uses the post-critical reflection P waves at the Moho generated by teleseismic S waves at the free surface near the receivers (SsPmp). However, observed SsPmp sometimes have significantly lower amplitude than predicted, raising doubts among the seismic community on the theoretical basis of the method. With over two decades of continuous digital broadband records and major subduction zones in the range of 30-50 degrees, the Yellowknife Array in northern Canada provides a rich opportunity for observation of post-critical SsPmp. We analyze S wave coda of events with epicenter distances of 30-50°, and pay special attention to earthquakes in a narrow azimuth range that ­­­encompasses the Kamchatka Peninsula. Among 21 events with strong direct S energy on the radial components, we observe significant variation of SsPmp energy. After associating the SsPmp energy with the virtual source location of each event, we observe a general trend of decreasing SsPmp energy from NE to SW. As the trend coincides with the transition from exposed basement of the Slave Craton to Paleozoic platform covered by Phanerozoic sediment, we interpret the decreasing SsPmp energy as a result of lower S velocity at the virtual sources, which reduces S-to-P reflection coefficients. We plan to include more events from the Aleutian Islands, the virtual sources of which are primarily located in the Paleozoic platform. This will allow us to further investigate the relationship between SsPmp amplitude and near-surface velocity.

RNA-processing proteins regulate Mec1/ATR activation by promoting generation of RPA-coated ssDNA.

Science.gov (United States)

Manfrini, Nicola; Trovesi, Camilla; Wery, Maxime; Martina, Marina; Cesena, Daniele; Descrimes, Marc; Morillon, Antonin; d'Adda di Fagagna, Fabrizio; Longhese, Maria Pia

2015-02-01

Eukaryotic cells respond to DNA double-strand breaks (DSBs) by activating a checkpoint that depends on the protein kinases Tel1/ATM and Mec1/ATR. Mec1/ATR is activated by RPA-coated single-stranded DNA (ssDNA), which arises upon nucleolytic degradation (resection) of the DSB. Emerging evidences indicate that RNA-processing factors play critical, yet poorly understood, roles in genomic stability. Here, we provide evidence that the Saccharomyces cerevisiae RNA decay factors Xrn1, Rrp6 and Trf4 regulate Mec1/ATR activation by promoting generation of RPA-coated ssDNA. The lack of Xrn1 inhibits ssDNA generation at the DSB by preventing the loading of the MRX complex. By contrast, DSB resection is not affected in the absence of Rrp6 or Trf4, but their lack impairs the recruitment of RPA, and therefore of Mec1, to the DSB. Rrp6 and Trf4 inactivation affects neither Rad51/Rad52 association nor DSB repair by homologous recombination (HR), suggesting that full Mec1 activation requires higher amount of RPA-coated ssDNA than HR-mediated repair. Noteworthy, deep transcriptome analyses do not identify common misregulated gene expression that could explain the observed phenotypes. Our results provide a novel link between RNA processing and genome stability. © 2014 The Authors.

Fabrication of nanoporous Sr incorporated TiO{sub 2} coating on 316L SS: Evaluation of bioactivity and corrosion protection

Energy Technology Data Exchange (ETDEWEB)

Anne Pauline, S. [Department of Chemistry, Anna University, Chennai 600025 (India); Kamachi Mudali, U. [Corrosion Science and Technology Section, IGCAR, Kalpakkam 603102 (India); Rajendran, N., E-mail: nrajendran@annauniv.edu [Department of Chemistry, Anna University, Chennai 600025 (India)

2013-10-01

In this paper, nanoporous TiO{sub 2} and Sr-incorporated TiO{sub 2} coated 316L SS were prepared by sol–gel methodology. The effect of Sr incorporation into TiO{sub 2} coating on bioactivity and corrosion resistance was investigated. Attenuated total reflectance–Fourier transform infrared (ATR–FTIR) spectroscopy, X-ray diffraction analysis (XRD), atomic force microscopy (AFM), scanning electron microscopy (SEM) and energy dispersive X-ray analysis (EDX) results obtained after in vitro bioactivity test confirm the excellent growth of crystalline hydroxyapatite (HAp) over nanoporous Sr-incorporated TiO{sub 2} coated 316L SS which may be attributed to the slow and steady release of Sr ions from the coatings. The electrochemical evaluation of the coatings confirms that Sr-incorporated TiO{sub 2} coating offer excellent protection to 316L SS by acting as a barrier layer. The results showed that the incorporation of Sr enhanced both bioactivity and corrosion resistance of 316L SS. Hence Sr-incorporated TiO{sub 2} coated 316L SS is a promising material for orthopaedic implant applications. - Highlights: • Nanoporous Sr-incorporated TiO{sub 2} coatings were successfully fabricated on 316L SS. • The coatings have excellent adhesion to the substrate and appreciable Vickers micro hardness value. • Sr-incorporated TiO{sub 2} coated specimens exhibited excellent hydroxyapatite growth due to slow release of Sr from the coating. • Sr incorporation enhances the corrosion resistance of TiO{sub 2} coating.

Book Reviews | Naidu | South African Medical Journal

African Journals Online (AJOL)

Book Review 1. Book Title: The Histogenesis of Thyroid Cancer. Book Author: N Simionescu. Illustrated. £9.0.0. London: William Heinemann Medical Books. 1970. Book Review 2. Book Title: The Hypertensive Vascular Crisis. An experimental study. Book Author: F.B. Byrom. Illustrated. £1.10.0. London: William Heinemann ...

Elastic-plastic analysis of the SS-3 tensile specimen

International Nuclear Information System (INIS)

Majumdar, S.

1998-01-01

Tensile tests of most irradiated specimens of vanadium alloys are conducted using the miniature SS-3 specimen which is not ASTM approved. Detailed elastic-plastic finite element analysis of the specimen was conducted to show that, as long as the ultimate to yield strength ratio is less than or equal to 1.25 (which is satisfied by many irradiated materials), the stress-plastic strain curve obtained by using such a specimen is representative of the true material behavior

Antibacterial effects, biocompatibility and electrochemical behavior of zinc incorporated niobium oxide coating on 316L SS for biomedical applications

Science.gov (United States)

Pradeep PremKumar, K.; Duraipandy, N.; Manikantan Syamala, Kiran; Rajendran, N.

2018-01-01

In the present study, Nb2O5 (NZ0) composite coatings with various concentrations of zinc (NZ2, NZ4 & NZ6) are produced on 316L SS by sol-gel method with the aim of improving its antibacterial activity, bone formability and corrosion resistance properties. This work studied the surface characterization of NZ0, NZ2, NZ4 & NZ6 coated 316L SS by ATR-FTIR, XRD, HR-SEM with EDAX. The synthesized coatings were different in the morphological aspects, NZ0 shows mesoporous morphology whereas irregular cluster like morphology was observed for the zinc incorporated coatings. The chemical composition of the NZ0 and NZ4 composite coatings were studied by XPS and the results revealed that the zinc exist as ZnO and Nb as Nb2O5 in the coatings. The increase in the concentration of zinc in Nb2O5 increases the hydrophilic nature identified by water contact angle studies. The potentiodynamic polarization studies in simulated body fluid reveals the increase in polarization resistance with decrease in current density (icorr) and electrochemical impedance spectroscopic studies with increase in charge transfer resistance (Rct) and double layer capacitance (Qdl) were observed for NZ4 coated 316L SS. The inhibition of Staphylococcus aureus and Escherichia coli bacteria were identified for NZ4 coated 316L SS by bacterial viability studies. The NZ4 coated 316L SS showed better Osseo-integration by spreading the MG 63 osteoblast cells. The study results imply that zinc incorporated Nb2O5 (NZ4) composite coating exhibits antibacterial activity and also enhance the corrosion resistance and biocompatibility of the 316L SS.

IFNL4 ss469415590 Variant Is Associated with Treatment Response in Japanese HCV Genotype 1 Infected Individuals Treated with IFN-Including Regimens

Directory of Open Access Journals (Sweden)

Tatsuo Miyamura

2014-01-01

Full Text Available Aim. Eradication of hepatitis C virus (HCV is still challenging even if interferon- (IFN- free regimens with direct-acting antiviral agents (DAAs for HCV-infected individuals are available in clinical practice. IFNL4 is a newly described protein, associated with human antiviral defenses. We investigated whether IFNL4 ss469415590 variant has an effect on the prediction of treatment response in HCV-infected patients treated with IFN-including regimens. Patients and Methods. In all, 185 patients infected with HCV genotype 1 treated with peg-IFN plus ribavirin, with or without telaprevir, were genotyped for IFNL4 ss469415590. We retrospectively investigated whether the role of IFNL4 ss469415590 variant and other factors could predict sustained virological response (SVR in Japanese patients infected with HCV genotype 1. Results. There were 65.7%, 31.5%, and 2.8% patients in the IFNL4 ss469415590 TT/TT, TT/-G, and -G/-G groups, respectively. SVR rates were 82.1% or 49.3% in patients treated with peg-IFN plus ribavirin with or without telaprevir, respectively. IFNL4 ss469415590 variant and HCV viral loads or IFNL4 ss469415590 variant and early virological response were better predictors of SVR in patients treated with peg-IFN plus ribavirin with or without telaprevir, respectively. Conclusion. In the era of DAAs, measurement of IFNL4 ss469415590 variant could help the prediction of SVR in Japanese HCV genotype 1 infected individuals treated with IFN-including regimens.

Using long ssDNA polynucleotides to amplify STRs loci in degraded DNA samples

Science.gov (United States)

Pérez Santángelo, Agustín; Corti Bielsa, Rodrigo M.; Sala, Andrea; Ginart, Santiago; Corach, Daniel

2017-01-01

Obtaining informative short tandem repeat (STR) profiles from degraded DNA samples is a challenging task usually undermined by locus or allele dropouts and peak-high imbalances observed in capillary electrophoresis (CE) electropherograms, especially for those markers with large amplicon sizes. We hereby show that the current STR assays may be greatly improved for the detection of genetic markers in degraded DNA samples by using long single stranded DNA polynucleotides (ssDNA polynucleotides) as surrogates for PCR primers. These long primers allow a closer annealing to the repeat sequences, thereby reducing the length of the template required for the amplification in fragmented DNA samples, while at the same time rendering amplicons of larger sizes suitable for multiplex assays. We also demonstrate that the annealing of long ssDNA polynucleotides does not need to be fully complementary in the 5’ region of the primers, thus allowing for the design of practically any long primer sequence for developing new multiplex assays. Furthermore, genotyping of intact DNA samples could also benefit from utilizing long primers since their close annealing to the target STR sequences may overcome wrong profiling generated by insertions/deletions present between the STR region and the annealing site of the primers. Additionally, long ssDNA polynucleotides might be utilized in multiplex PCR assays for other types of degraded or fragmented DNA, e.g. circulating, cell-free DNA (ccfDNA). PMID:29099837

Economic impacts of the S.S. Glacier Bay oil spill: Social and economic studies. Technical report (Final)

International Nuclear Information System (INIS)

Burden, P.; Isaacs, J.; Richardson, J.; Braund, S.; Witten, E.

1990-11-01

On July 2, 1987, an oil spill occurred in Cook Inlet when the S.S. Glacier Bay hit a submerged obstacle while enroute to Kenai Pipeline Company facilities to offload oil. The 1987 commercial fishery in Cook Inlet was barely underway when the S.S. Glacier Bay oil spill occurred, and the largest salmon return in history was moving up the inlet. The sockeye salmon run alone totaled over 12 million, providing a seasonal catch of 9.25 million salmon. The 1987 sport fishery in Cook Inlet was in mid-season at the time of the spill. The S.S. Glacier Bay oil spill represents an opportunity to study the economic impacts of an oil spill event in Alaska, particularly with regard to commercial fishing impacts and the public costs of cleanup. The report evaluates the existing information on the spill, response measures, and economic impacts, and adds discussions with individuals and groups involved in or affected by the spill to this data base. The report reviewed accounts of the oil spill and its costs; identified types and sources of data, developed protocol, and contacted groups and people for data collection and verification; and described, analyzed, and prepared reports of the economic effects of the S.S. Glacier Bay oil spill

Knowledge horizons: the present and the promise of knowledge management

National Research Council Canada - National Science Library

Chauvel, Daniele; Despres, Charles

2000-01-01

... what has been written, Butterworth- Heinemann prints its books on acid-free paper whenever possible. Butterworth- Heinemann supports the efforts of American Forests and the Global ReLeaf program in its campaign for the betterment of trees, forests, and our environment. Library of Congress Cataloging-in-Publication Data Knowledge horizons : the present ...

Continuous cooling and low temperature sensitization of AISI types 316 SS and 304 SS with different degrees of cold work

Energy Technology Data Exchange (ETDEWEB)

Parvathavarthini, N.; Dayal, R.K.; Gnanamoorthy, J.B. (Indira Gandhi Centre for Atomic Research, Kalpakkam (India). Metallurgy and Materials Programme); Seshadri, S.K. (Indian Inst. of Tech., Madras (India). Dept. of Metallurgical Engineering)

This paper presents the results of investigations carried out to study the sensitization behaviour of AISI Types 316 SS and 304 SS with various degrees of cold work ranging from 0 to 25%. Initially Time-Temperature-Sensitization (TTS) diagrams were established using ASTM standard A262 Practice A and E tests. From these diagrams it was found that the rate of sensitization and overall susceptibility to intergranular corrosion increases up to 15% cold work and above that starts decreasing. Desensitization was observed to be faster for higher levels of cold work, especially in the higher sensitization temperature range. From the TTS diagrams, the critical linear cooling rate below which sensitization occurs was calculated. From these data, Continuous Cooling Sensitization (CCS) diagrams were established. The results show that as the degree of cold work increases up to 15%, time needed for sensitization decreases and hence faster cooling rates must be used in order to avoid sensitization. At temperatures sufficiently below the nose temperature of the TTS diagram, log t versus 1/T plots follow a linear relationship where t is the time needed for the onset of sensitization at temperature T. From the slope, the apparent activation energy for sensitization was estimated. The validity of extrapolating these linear plots to lower temperatures (725 to 775 K) (which lie in the operating temperature regime of fast reactors) has been verified by experiment. The effect of heat treatment and microstructure on the Low Temperature Sensitization (LTS) behaviour was investigated. The results indicate that carbides of optimum size and distribution are the essential pre-requisites for LTS and cold work enhances susceptibility of stainless steels to LTS. (orig.).

Varttuneet ja ikäsyrjintä työelämässä

OpenAIRE

Viitasalo, Niina

2015-01-01

Työelämän ikäsyrjintä esteenä pitkille työurille Työelämässä esiintyvä ikäsyrjintä on ristiriidassa työurien pidentämisen kanssa. Ikäsyrjintää kokeneilla on jopa kaksinkertainen pitkien sairauspoissaolojen riski 3 vuoden seuranta-aikana. Eniten ikäsyrjintäkokemuksia on yli 55-vuotiailla naisilla, heistä lähes 8 prosenttia on kokenut ikäsyrjintää työssään. Ikäsyrjintä ilmenee työpaikkojen arjessa varttuneiden torjuntana, painostamisena, vähättelynä tai eristämisenä. Ikäsyrjintä näyttäytyy ...

High-frequency genome editing using ssDNA oligonucleotides with zinc-finger nucleases

DEFF Research Database (Denmark)

Chen, Fuqiang; Pruett-Miller, Shondra M; Huang, Yuping

2011-01-01

Zinc-finger nucleases (ZFNs) have enabled highly efficient gene targeting in multiple cell types and organisms. Here we describe methods for using simple ssDNA oligonucleotides in tandem with ZFNs to efficiently produce human cell lines with three distinct genetic outcomes: (i) targeted point...

Highlights from the Functional Single Nucleotide Polymorphisms Associated with Human Muscle Size and Strength or FAMuSS Study

Directory of Open Access Journals (Sweden)

Linda S. Pescatello

2013-01-01

Full Text Available The purpose of the Functional Single Nucleotide Polymorphisms Associated with Human Muscle Size and Strength study or FAMuSS was to identify genetic factors that dictated the response of health-related fitness phenotypes to resistance exercise training (RT. The phenotypes examined were baseline muscle strength and muscle, fat, and bone volume and their response to RT. FAMuSS participants were 1300 young (24 years, healthy men (42% and women (58% that were primarily of European-American descent. They were genotyped for ~500 polymorphisms and completed the Paffenbarger Physical Activity Questionnaire to assess energy expenditure and time spent in light, moderate, and vigorous intensity habitual physical activity and sitting. Subjects then performed a 12-week progressive, unilateral RT program of the nondominant arm with the dominant arm used as a comparison. Before and after RT, muscle strength was measured with the maximum voluntary contraction and one repetition maximum, while MRI measured muscle, fat, and bone volume. We will discuss the history of how FAMuSS originated, provide a brief overview of the FAMuSS methods, and summarize our major findings regarding genotype associations with muscle strength and size, body composition, cardiometabolic biomarkers, and physical activity.

Hybridization of plant virus ssRNAs Transferred to Hybond N membrane

International Nuclear Information System (INIS)

Kudela, O.; Kudelova, K.; Plaschke-Jakubik, K.

1998-01-01

In this paper we present a protocol for the non-denaturating agarose gel electrophoresis of plant virus ssRNAs, their blotting onto Hybond N membrane, and hybridization with [alpha 32 P]dNTP-labelled cDNA probe. The protocol is not pretentious on technical equipment, omits denaturation and neutralization steps and some chemical required in other modifications. (authors)

Sour grapes: multiple groundwater enclosures in Morocco's Saïss region

NARCIS (Netherlands)

Bossenbroek, L.; Kuper, M.; Zwarteveen, M.Z.

2017-01-01

While driving to the douar 1 Ait Ali in the agricultural plain of the Saïss in Morocco, we always passed by a fallow land plot of approximately 10 hectares. We passed this plot without noticing much, until the day we spotted a Syrian tube-well driller with a checked red scarf on his head and his

Réponse équilibrée aux besoins en eau dans le bassin du Saïss au ...

International Development Research Centre (IDRC) Digital Library (Canada)

ACCA

par les changements climatiques. Ce projet du programme ACCA met l'accent sur l'agriculture, à l'origine de plus de 82 % de la consommation d'eau dans le bassin. Réponse équilibrée aux besoins en eaudans le bassin du Saïss au Maroc. De petits exploitants agricoles du bassin du Saïss font face aux pénuries d'eau ...

The Type IX Secretion System (T9SS: Highlights and Recent Insights into Its Structure and Function

Directory of Open Access Journals (Sweden)

Anna M. Lasica

2017-05-01

Full Text Available Protein secretion systems are vital for prokaryotic life, as they enable bacteria to acquire nutrients, communicate with other species, defend against biological and chemical agents, and facilitate disease through the delivery of virulence factors. In this review, we will focus on the recently discovered type IX secretion system (T9SS, a complex translocon found only in some species of the Bacteroidetes phylum. T9SS plays two roles, depending on the lifestyle of the bacteria. It provides either a means of movement (called gliding motility for peace-loving environmental bacteria or a weapon for pathogens. The best-studied members of these two groups are Flavobacterium johnsoniae, a commensal microorganism often found in water and soil, and Porphyromonas gingivalis, a human oral pathogen that is a major causative agent of periodontitis. In P. gingivalis and some other periodontopathogens, T9SS translocates proteins, especially virulence factors, across the outer membrane (OM. Proteins destined for secretion bear a conserved C-terminal domain (CTD that directs the cargo to the OM translocon. At least 18 proteins are involved in this still enigmatic process, with some engaged in the post-translational modification of T9SS cargo proteins. Upon translocation across the OM, the CTD is removed by a protease with sortase-like activity and an anionic LPS is attached to the newly formed C-terminus. As a result, a cargo protein could be secreted into the extracellular milieu or covalently attached to the bacterial surface. T9SS is regulated by a two-component system; however, the precise environmental signal that triggers it has not been identified. Exploring unknown systems contributing to bacterial virulence is exciting, as it may eventually lead to new therapeutic strategies. During the past decade, the major components of T9SS were identified, as well as hints suggesting the possible mechanism of action. In addition, the list of characterized cargo

The Type IX Secretion System (T9SS): Highlights and Recent Insights into Its Structure and Function

Science.gov (United States)

Lasica, Anna M.; Ksiazek, Miroslaw; Madej, Mariusz; Potempa, Jan

2017-01-01

Protein secretion systems are vital for prokaryotic life, as they enable bacteria to acquire nutrients, communicate with other species, defend against biological and chemical agents, and facilitate disease through the delivery of virulence factors. In this review, we will focus on the recently discovered type IX secretion system (T9SS), a complex translocon found only in some species of the Bacteroidetes phylum. T9SS plays two roles, depending on the lifestyle of the bacteria. It provides either a means of movement (called gliding motility) for peace-loving environmental bacteria or a weapon for pathogens. The best-studied members of these two groups are Flavobacterium johnsoniae, a commensal microorganism often found in water and soil, and Porphyromonas gingivalis, a human oral pathogen that is a major causative agent of periodontitis. In P. gingivalis and some other periodontopathogens, T9SS translocates proteins, especially virulence factors, across the outer membrane (OM). Proteins destined for secretion bear a conserved C-terminal domain (CTD) that directs the cargo to the OM translocon. At least 18 proteins are involved in this still enigmatic process, with some engaged in the post-translational modification of T9SS cargo proteins. Upon translocation across the OM, the CTD is removed by a protease with sortase-like activity and an anionic LPS is attached to the newly formed C-terminus. As a result, a cargo protein could be secreted into the extracellular milieu or covalently attached to the bacterial surface. T9SS is regulated by a two-component system; however, the precise environmental signal that triggers it has not been identified. Exploring unknown systems contributing to bacterial virulence is exciting, as it may eventually lead to new therapeutic strategies. During the past decade, the major components of T9SS were identified, as well as hints suggesting the possible mechanism of action. In addition, the list of characterized cargo proteins is

Biodegradable mixed MPEG-SS-2SA/TPGS micelles for triggered intracellular release of paclitaxel and reversing multidrug resistance

Directory of Open Access Journals (Sweden)

Dong K

2016-10-01

Full Text Available Kai Dong,1 Yan Yan,2 Pengchong Wang,2 Xianpeng Shi,2 Lu Zhang,2 Ke Wang,2 Jianfeng Xing,2 Yalin Dong1 1Department of Pharmacy, The First Affiliated Hospital of Xi’an Jiaotong University, 2School of Pharmacy, Xi’an Jiaotong University, Xi’an, Shaanxi, People’s Republic of China Abstract: In this study, a type of multifunctional mixed micelles were prepared by a novel biodegradable amphiphilic polymer (MPEG-SS-2SA and a multidrug resistance (MDR reversal agent (D-α-tocopheryl polyethylene glycol succinate, TPGS. The mixed micelles could achieve rapid intracellular drug release and reversal of MDR. First, the amphiphilic polymer, MPEG-SS-2SA, was synthesized through disulfide bonds between poly (ethylene glycol monomethyl ether (MPEG and stearic acid (SA. The structure of the obtained polymer was similar to poly (ethylene glycol-phosphatidylethanolamine (PEG-PE. Then the mixed micelles, MPEG-SS-2SA/TPGS, were prepared by MPEG-SS-2SA and TPGS through the thin film hydration method and loaded paclitaxel (PTX as the model drug. The in vitro release study revealed that the mixed micelles could rapidly release PTX within 24 h under a reductive environment because of the breaking of disulfide bonds. In cell experiments, the mixed micelles significantly inhibited the activity of mitochondrial respiratory complex II, also reduced the mitochondrial membrane potential, and the content of adenosine triphosphate, thus effectively inhibiting the efflux of PTX from cells. Moreover, in the confocal laser scanning microscopy, cellular uptake and 3-(4,5-dimethyl-thiazol-2-yl-2,5-diphenyl-tetrazolium bromide assays, the MPEG-SS-2SA/TPGS micelles achieved faster release and more uptake of PTX in Michigan Cancer Foundation-7/PTX cells and showed better antitumor effects as compared with the insensitive control. In conclusion, the biodegradable mixed micelles, MPEG-SS-2SA/TPGS, could be potential vehicles for delivering hydrophobic chemotherapeutic drugs in

Electropolymerization of camphorsulfonic acid doped conductive polypyrrole anti-corrosive coating for 304SS bipolar plates

Science.gov (United States)

Jiang, Li; Syed, Junaid Ali; Gao, Yangzhi; Zhang, Qiuxiang; Zhao, Junfeng; Lu, Hongbin; Meng, Xiangkang

2017-12-01

Conductive polymer coating doped with large molecular organic acid is an alternative method used to protect stainless steel (SS) bipolar plates in proton exchange membrane fuel cells (PEMFCs). However, it is difficult to select the proper doping acid, which improves the corrosion resistance of the coating without affecting its conductivity. In this study, large spatial molecular group camphorsulfonic acid (CSA) doped polypyrrole (PPY) conductive coating was prepared by galvanostatic electropolymerization on 304SS. The electrochemical properties of the coating were evaluated in 0.1 M H2SO4 solution in order to simulate the PEMFC service environment. The results indicate that the coating increased the corrosion potential and shifted Ecorr towards more positive value, particularly the jcorr value of PPY-CSA coated 304SS was dropped from 97.3 to 0.00187 μA cm-2. The long-term immersion tests (660 h) show that the PPY-CSA coating exhibits better corrosion resistance in comparison with the small acid (SO42-) doped PPY-SO42- or PPY/PPY-SO42- coatings. Moreover, the PPY-CSA coating presents low contact resistance and maintains strong corrosion resistance during the prolonged exposure time due to barrier effect and anodic protection.

Defining the Content of an Online Sexual Health Intervention: The MenSS Website.

Science.gov (United States)

Webster, Rosie; Gerressu, Makeda; Michie, Susan; Estcourt, Claudia; Anderson, Jane; Ang, Chee Siang; Murray, Elizabeth; Rait, Greta; Stephenson, Judith; Bailey, Julia V

2015-07-03

Health promotion and risk reduction are essential components of sexual health care. However, it can be difficult to prioritize these within busy clinical services. Digital interventions may provide a new method for supporting these. The MenSS (Men's Safer Sex) website is an interactive digital intervention developed by a multidisciplinary team, which aims to improve condom use in men who have sex with women (MSW). This paper describes the content of this intervention, and the rationale for it. Content was informed by a literature review regarding men's barriers to condom use, workshops with experts in sexual health and technology (N=16) and interviews with men in sexual health clinics (N=20). Data from these sources were analyzed thematically, and synthesized using the Behavior Change Wheel framework. The MenSS intervention is a website optimized for delivery via tablet computer within a clinic waiting room setting. Key targets identified were condom use skills, beliefs about pleasure and knowledge about risk. Content was developed using behavior change techniques, and interactive website features provided feedback tailored for individual users. This paper provides a detailed description of an evidence-based interactive digital intervention for sexual health, including how behavior change techniques were translated into practice within the design of the MenSS website. Triangulation between a targeted literature review, expert workshops, and interviews with men ensured that a range of potential influences on condom use were captured.

Kestävä kehitys ammattikeittiössä - Case Päiväkoti Lumikki

OpenAIRE

Holopainen, Joonas

2011-01-01

Opinnäytetyöni oli kestävä kehitys ammattikeittiössä – case päiväkoti Lumikki. Toimeksiantaja ja yhteistyökumppani oli Tiinan Taaperot Ky. Opinnäytetyö hyödyntää KestITÄ -hanketta, eli Kestävyyttä Itä-Suomen ruokapalveluihin -hanketta. Opinnäytetyön tavoitteena oli selvittää, mitä on kestävä kehitys ja miten se toteutuu päiväkoti Lumikin keittiössä sekä miten sitä voisi parantaa keittiön toiminnassa. Lisäksi tarkoituksena oli suunnitella ruokalista yhden viikon lounasruoista siten, että k...

Efficient Generation of Orthologous Point Mutations in Pigs via CRISPR-assisted ssODN-mediated Homology-directed Repair

Directory of Open Access Journals (Sweden)

Kankan Wang

2016-01-01

Full Text Available Precise genome editing in livestock is of great value for the fundamental investigation of disease modeling. However, genetically modified pigs carrying subtle point mutations were still seldom reported despite the rapid development of programmable endonucleases. Here, we attempt to investigate single-stranded oligonucleotides (ssODN mediated knockin by introducing two orthologous pathogenic mutations, p.E693G for Alzheimer's disease and p.G2019S for Parkinson's disease, into porcine APP and LRRK2 loci, respectively. Desirable homology-directed repair (HDR efficiency was achieved in porcine fetal fibroblasts (PFFs by optimizing the dosage and length of ssODN templates. Interestingly, incomplete HDR alleles harboring partial point mutations were observed in single-cell colonies, which indicate the complex mechanism of ssODN-mediated HDR. The effect of mutation-to-cut distance on incorporation rate was further analyzed by deep sequencing. We demonstrated that a mutation-to-cut distance of 11 bp resulted in a remarkable difference in HDR efficiency between two point mutations. Finally, we successfully obtained one cloned piglet harboring the orthologous p.C313Y mutation at the MSTN locus via somatic cell nuclear transfer (SCNT. Our proof-of-concept study demonstrated efficient ssODN-mediated incorporation of pathogenic point mutations in porcine somatic cells, thus facilitating further development of disease modeling and genetic breeding in pigs.

Constraints on particle acceleration in SS433/W50 from MAGIC and H.E.S.S. observations

OpenAIRE

MAGIC Collaboration; Ahnen, M. L.; Ansoldi, S.; Antonelli, L. A.; Arcaro, C.; Babić, A.; Banerjee, B.; Bangale, P.; de Almeida, U. Barres; Barrio, J. A.; González, J. Becerra; Bednarek, W.; Bernardini, E.; Berti, A.; Biasuzzi, B.

2017-01-01

The large jet kinetic power and non-thermal processes occurring in the microquasar SS 433 make this source a good candidate for a very high-energy (VHE) gamma-ray emitter. Gamma-ray fluxes have been predicted for both the central binary and the interaction regions between jets and surrounding nebula. Also, non-thermal emission at lower energies has been previously reported. We explore the capability of SS 433 to emit VHE gamma rays during periods in which the expected flux attenuation due to ...

Constraints on particle acceleration in SS433/W50 from MAGIC and H.E.S.S. observations

OpenAIRE

Ahnen, M. L.; Ansoldi, S.; Bednarek, W.; Paiano, S.; Palacio, J.; Paneque, D.; Paoletti, R.; Paredes, J. M.; Paredes-Fortuny, X.; Pedaletti, G.; Peresano, M.; Perri, L.; Persic, M.; Bernardini, E.; Prada Moroni, P. G.

2018-01-01

Context. The large jet kinetic power and non-thermal processes occurring in the microquasar SS 433 make this source a good candidate for a very high-energy (VHE) gamma-ray emitter. Gamma-ray fluxes above the sensitivity limits of current Cherenkov telescopes have been predicted for both the central X-ray binary system and the interaction regions of SS 433 jets with the surrounding W50 nebula. Non-thermal emission at lower energies has been previously reported, indicating that efficient partic...

The Telehealth Satisfaction Scale (TeSS): Reliability, validity, and satisfaction with telehealth in a rural memory clinic population

Science.gov (United States)

Morgan, Debra G; Kosteniuk, Julie; Stewart, Norma; O’Connell, Megan E; Karunanyake, Chandima; Beever, Rob

2015-01-01

Introduction Patient satisfaction is a key aspect of quality of care and can inform continuous quality improvement. Of the few studies that have reported on patient satisfaction with telehealth in programs aimed at individuals with memory problems, none has reported on the psychometric properties of the user satisfaction scales employed. Methods We evaluated the construct validity and internal consistency reliability of the Telehealth Satisfaction Scale (TeSS), a 10-item scale adapted for use in a Rural and Remote Memory Clinic (RRMC). The RRMC is a one-stop interprofessional clinic for rural and remote seniors with suspected dementia, located in a tertiary care hospital. Telehealth videoconferencing is used for pre-clinic assessment and for follow-up. Patients and caregivers completed the TeSS after each telehealth appointment. With data from 223 patients, exploratory factor analysis was conducted using the principal components analysis extraction method. Results The eigenvalue for the first factor (5.2) was greater than 1 and much larger than the second eigenvalue (.92), supporting a one-factor solution that was confirmed by the scree plot. The total variance explained by factor 1 was 52.1%. Factor loadings (range 0.54 – 0.84) were above recommended cutoffs. The TeSS items demonstrated high internal consistency reliability (Cronbach’s alpha = 0.90). Satisfaction scores on the TeSS items ranged from 3.43 to 3.72 on a 4-point Likert scale, indicating high satisfaction with telehealth. Conclusions The study findings demonstrate high user satisfaction with telehealth in a rural memory clinic, and sound psychometric properties of the TeSS in this population. PMID:25272141

Complex, variable structure of stationary lines in SS433

Energy Technology Data Exchange (ETDEWEB)

Falomo, R.; Boksenberg, A.; Tanzi, E.G.; Tarenghi, M.; Treves, A.

1987-01-15

On 1979 June 3-6, a number of spectra of SS433 were obtained using the UCL Image Photon Counting System on the 3.6-m telescope of the European Southern Observatory, La Silla, Chile. The stationary H..cap alpha.. and He I lambdalambda5875, 6678 and 7065 lines have a complex structure which on June 4-5 exhibited a central feature accompanied by two equally displaced (+- 1000 km s/sup -1/) side components. Variability of the line profile and equivalent width is observed on time-scales as short as a quarter of an hour.

Periodicity Analysis of X-ray Light Curves of SS 433

Science.gov (United States)

Wang, Jun-yi; Lu, Xiang-long; Zhao, Qiu-wen; Dong, Dian-qiao; Lao, Bao-qiang; Lu, Yang; Wei, Yan-heng; Wu, Xiao-cong; An, Tao

2017-01-01

SS 433 is sofar the unique X-ray binary that has the simultaneously detected orbital period, super-orbital period, and nutation period, as well as a bidirectional spiral jet. The study on its X-ray light variability is helpful for understanding the dynamic process of the system, and the correlations between the different wavebands. In this paper, two time-series analysis techniques, i.e., the Lomb-Scargle periodogram and weighted wavelet Z-transform, are employed to search for the periods in the Swift/BAT (Burst Alert Telescope) (15-50 keV) and RXTE/ASM (Rose X-ray Timing Explorer/All Sky Monitor) (1.5-3, 3- 4, and 5-12 keV) light curves of SS 433, and the Monte Carlo simulation is performed for the obtained periodical components. For the 15-50 keV energy band, five significant periodical components are detected, which are P1(∼6.29 d), P2 (∼6.54 d), P3 (∼13.08 d), P4 (∼81.50 d), and P5 (∼162.30 d). For the 3-5 and 5-12 keV energy bands, the periodical components P3 (∼13 d) and P5 (∼162 d) are detected in both energy bands. However, for the 1.5-3 keV energy band, no significant periodic signal is detected. P5 is the strongest periodic signal in the power spectrum for all the energy bands of 3-5, 5-12, and 15-50 keV, and it is consistent with the previous result obtained from the study of optical light curves. Furthermore, in combination with the radio spiral jet of SS 433, it is suggested that the X-ray and optical variability of P5 (∼162 d) is probably related to the precession of its relativistic jet. The high correlation between the X-ray and optical light curves may also imply that the X-ray and optical radiations are of the same physical origin. P3 shows a good agreement with the orbital period (∼13.07 d) obtained by the previous study, and P2 and P4 are respectively the high-frequency harmonics of P3 and P5. P1 is detected only in the power spectrum of the 15-50 keV energy band, and it is consistent with the nutation period of the system. As

Peripheral vascular response to mild indirect cooling in patients with homozygous sickle cell (SS) disease and the frequency of painful crisis.

Science.gov (United States)

Mohan, J; Marshall, J M; Reid, H L; Thomas, P W; Hambleton, I; Serjeant, G R

1998-02-01

1. In homozygous sickle cell (SS) disease, skin cooling is a common precipitating factor of the painful crisis which is associated with avascular necrosis of active bone marrow. Since skin cooling does not directly induce sickling, we have investigated the nature of the reflex vascular responses to mild cooling in SS patients in a steady state of the disease and compared them with their history of painful crises. 2. Experiments were performed in Jamaica on 60 male SS patients and 30 matched control subjects with normal haemoglobin (AA) genotype. Forearm blood flow (FBF) was measured by venous occlusion plethysmography and mean arterial pressure (MAP) by a Finapres device: forearm vascular resistance (FVR) was calculated as MAP/FBF. Cutaneous erythrocyte flux in forearm and hand was monitored by a laser Doppler meter. The contralateral hand was immersed in cool water at 16 degrees C for 2 min, 6 times, at random intervals of 0.5-3 min. 3. The first cool immersion evoked an increase in MAP, cutaneous vasoconstriction and a net increase in FVR in both AA and SS subjects. However, the direction of change in FVR varied between individuals such that 18 AA subjects showed an increase in FVR (constrictor group) while 12 showed a decrease in FVR, indicating vasodilatation in forearm muscle (dilator group). In contrast, 50 SS subjects showed an increase in FVR and only 10 showed a decrease in FVR. The proportion of subjects who showed net vasoconstriction was significantly greater in the SS than in the AA group (83% versus 60%, P = 0.03, chi 2 test). 4. By the sixth cool stimulus, the 'dilator' group of AA subjects showed no change in FVR while the 'dilator' group of SS patients showed an increase in FVR. We suggest that forearm muscle vasodilatation was the characteristic component of the alerting/defence response to novel or noxious stimuli which habituates on repetition. 5. In the whole group of SS patients, baseline values of cutaneous vascular resistance and FVR

IDIS-menetelmäsalkku työntekijän ammattitaidon vahvistajana lastensuojelun identiteettityössä

OpenAIRE

Palm, David

2014-01-01

Opinnäytetyön tarkoituksena oli vahvistaa lastensuojelutyön työntekijän ammattitaitoa lasten ja nuorten kanssa tehtävässä identiteettityössä. Tavoitteena oli IDIS-menetelmäsalkun avulla toteuttaa ja testata pienryhmäkodissa toiminnallisia lasten ja nuorten arvon näkyväksi tekemiseen tähtääviä menetelmiä. IDIS-menetelmäsalkulla tavoiteltiin myös pysyvää toimintamenetelmää pienryhmäkodin arkeen. IDIS-menetelmäsalkku oli opinnäytetyön tekijän itse suunnittelema kokonaisuus. Tutkimukselli...

Laadun- ja riskienhallinta johtamisjärjestelmässä ISO 9001:2015 ja ISO 31000:2009 mukaisesti

OpenAIRE

Reinvall, Niko; Karhunen, Joonas

2015-01-01

Tämän opinnäytetyön tarkoituksena on tarkastella riskienhallinnan ja laadunhallintajärjestelmien suhdetta ISO 9001:2015 ja ISO 31000:2009-standardien viitekehyksessä, tutkimuskysymyksen ollessa ”miten ja miksi integroida ISO 9001 mukaista laadunhallintaa ja ISO 31000 mukaista riskienhallintaa organisaation johtamisjärjestelmään”. Lisäksi työssä tarkastellaan standardien välisiä yhtymäkohtia ja riskienhallinnan merkitystä ISO 9001:2015 mukaisessa laadunhallintajärjestelmässä. Työn tilaaja...

Periodic and secular changes in SS 433

International Nuclear Information System (INIS)

Collins, G.W.; Newsom, G.H.

1983-01-01

The recent history of SS 433 is reviewed with particular attention being given to the discovery of the periodic phenomena displayed by this object. Several periods ranging from days to months are established as being present in the spectrum of the ''moving'' lines as well as in other aspects of the emission from the object. In addition evidence for secular change in some of the defining parameters of the system is presented. Although these secular changes may eventually prove to be periodic on a rather long time scale, some interpretation of both the periodic and secular phenomena is possible. It is shown that it is possible to interpret all the known periodic phenomena in terms of a processing object responding to the time-varying torques that one would expect in a binary system

Ectopic Expression of the Coleus R2R3 MYB-Type Proanthocyanidin Regulator Gene SsMYB3 Alters the Flower Color in Transgenic Tobacco.

Directory of Open Access Journals (Sweden)

Qinlong Zhu

Full Text Available Proanthocyanidins (PAs play an important role in plant disease defense and have beneficial effects on human health. We isolated and characterized a novel R2R3 MYB-type PA-regulator SsMYB3 from a well-known ornamental plant, coleus (Solenostemon scutellarioides, to study the molecular regulation of PAs and to engineer PAs biosynthesis. The expression level of SsMYB3 was correlated with condensed tannins contents in various coleus tissues and was induced by wounding and light. A complementation test in the Arabidopsis tt2 mutant showed that SsMYB3 could restore the PA-deficient seed coat phenotype and activated expression of the PA-specific gene ANR and two related genes, DFR and ANS. In yeast two-hybrid assays, SsMYB3 interacted with the Arabidopsis AtTT8 and AtTTG1 to reform the ternary transcriptional complex, and also interacted with two tobacco bHLH proteins (NtAn1a and NtJAF13-1 and a WD40 protein, NtAn11-1. Ectopic overexpression of SsMYB3 in transgenic tobacco led to almost-white flowers by greatly reducing anthocyanin levels and enhancing accumulation of condensed tannins. This overexpression of SsMYB3 upregulated the key PA genes (NtLAR and NtANR and late anthocyanin structural genes (NtDFR and NtANS, but downregulated the expression of the final anthocyanin gene NtUFGT. The formative SsMYB3-complex represses anthocyanin accumulation by directly suppressing the expression of the final anthocyanin structural gene NtUFGT, through competitive inhibition or destabilization of the endogenous NtAn2-complex formation. These results suggested that SsMYB3 may form a transcription activation complex to regulate PA biosynthesis in the Arabidopsis tt2 mutant and transgenic tobacco. Our findings suggest that SsMYB3 is involved in the regulation of PA biosynthesis in coleus and has the potential as a molecular tool for manipulating biosynthesis of PAs in fruits and other crops using metabolic engineering.

Osaamalla organisaatiokulttuurin oivaltajaksi : Johtamisosaamista tehostetun palveluasumisen esimiestyössä

OpenAIRE

Oja, Ulla; Heinonen, Miia

2015-01-01

Tämä opinnäytetyö toteutettiin työelämälähtöisenä tutkimuksellisena kehittämishankkeena. Sen tarkoituksena oli edistää organisaatiokulttuuriin liittyvää johtamisosaamista Lahden kaupungin tehostetun palveluasumisen yksiköissä. Kehittämishankkeen ensimmäisenä tavoitteena oli kuvata, miten organisaatiokulttuuri ilmenee työyhteisössä ja millaista osaamista organisaatiokulttuurin johtaminen vaatii. Toisena tavoitteena oli tuottaa organisaatiokulttuurin johtamisosaamisen kehittämisalueet. Keh...

A novel silica nanotube reinforced ionic incorporated hydroxyapatite composite coating on polypyrrole coated 316L SS for implant application

Energy Technology Data Exchange (ETDEWEB)

Prem Ananth, K., E-mail: kpananth01@gmail.com [Department of Nanoscience and Technology, Bharathiar University, Coimbatore – 641 046 (India); Joseph Nathanael, A. [Department of Nano, Medical and Polymer Materials, Yeungnam University, Gyeongsan 712-749 (Korea, Republic of); Jose, Sujin P. [Department of Materials Science and Nano engineering, Rice University, Texas 77005 (United States); School of Physics, Madurai Kamaraj University, Madurai-625021 (India); Oh, Tae Hwan [Department of Nano, Medical and Polymer Materials, Yeungnam University, Gyeongsan 712-749 (Korea, Republic of); Mangalaraj, D. [Department of Nanoscience and Technology, Bharathiar University, Coimbatore – 641 046 (India)

2016-02-01

An attempt has been made to deposit a novel smart ion (Sr, Zn, Mg) substituted hydroxyapatite (I-HAp) and silica nanotube (SiNTs) composite coatings on polypyrrole (PPy) coated surgical grade 316L stainless steel (316L SS) to improve its biocompatibility and corrosion resistance. The I-HAp/SiNTS/PPy bilayer coating on 316L SS was prepared by electrophoretic deposition technique. Potentiodynamic polarization and electrochemical impedance spectroscopy (EIS) studies were carried out. These results confirmed the significant improvement of the corrosion resistance of the 316L SS alloy by the I-HAp/SiNTs/PPy bilayer composite coating. The adhesion strength and hardness test confirmed the anticipated mechanical properties of the composite. A low contact angle value revealed the hydrophilic nature. Inductively coupled plasma-atomic emission spectroscopy (ICP-AES) was used for the leach out analysis of the samples. Added to this, the bioactivity of the composite was analyzed by observing the apatite formation in the SBF solution for 7, 14, 21 and 28 days of incubation. An enhancement of in vitro osteoblast attachment and cell viability was observed, which could lead to the optimistic orthopedic and dental applications. - Highlights: • Polypyrrole (PPy) coated 316L SS substrates were fabricated using electrodeposition method. • A novel silica nanotube (SiNTs) and ionic substituted (Sr, Zn, Mg) hydroxyapatite composite (I-HAp) were prepared. • The composite (I-HAp/SiNTs) was coated on PPy coated 316L SS substrate using electrophoretic deposition. • These results are favorable for corrosion resistance and enhanced osteoblast cell attachment for bone formation.

Use of diffusion bonded SS-Al composite material in the development of neutron detectors

International Nuclear Information System (INIS)

Alex, Mary; Prasad, K.R.; Pappachan, A.L.; Grover, A.K.; Krishnan, J.; Derose, D.J.; Bhanumurthy, K.; Kale, G.B.

2005-01-01

The present paper describes the development of a SS-Al composite plate in-house at BARC by diffusion bonding technique. Details of the several tests carried out on the composite material and the use of the plate in the development of a boron lined neutron chamber for Dhruva reactor control instrumentation has been described. The bonded sample has withstood tensile strength test, leak test and thermal cycling test and the leak rate was observed to be less than 3 x 10 -10 stdcc/sec. The chamber with the composite material has been installed in Dhruva Basket C location and connected to the log rate safety channel. It has been working successfully for the past two years. The use of SS-Al composite material has improved the reliability and long-term performance of the detector. (author)

Protein dynamics of human RPA and RAD51 on ssDNA during assembly and disassembly of the RAD51 filament.

Science.gov (United States)

Ma, Chu Jian; Gibb, Bryan; Kwon, YoungHo; Sung, Patrick; Greene, Eric C

2017-01-25

Homologous recombination (HR) is a crucial pathway for double-stranded DNA break (DSB) repair. During the early stages of HR, the newly generated DSB ends are processed to yield long single-stranded DNA (ssDNA) overhangs, which are quickly bound by replication protein A (RPA). RPA is then replaced by the DNA recombinase Rad51, which forms extended helical filaments on the ssDNA. The resulting nucleoprotein filament, known as the presynaptic complex, is responsible for pairing the ssDNA with homologous double-stranded DNA (dsDNA), which serves as the template to guide DSB repair. Here, we use single-molecule imaging to visualize the interplay between human RPA (hRPA) and human RAD51 during presynaptic complex assembly and disassembly. We demonstrate that ssDNA-bound hRPA can undergo facilitated exchange, enabling hRPA to undergo rapid exchange between free and ssDNA-bound states only when free hRPA is present in solution. Our results also indicate that the presence of free hRPA inhibits RAD51 filament nucleation, but has a lesser impact upon filament elongation. This finding suggests that hRPA exerts important regulatory influence over RAD51 and may in turn affect the properties of the assembled RAD51 filament. These experiments provide an important basis for further investigations into the regulation of human presynaptic complex assembly. © The Author(s) 2016. Published by Oxford University Press on behalf of Nucleic Acids Research.

Differential alteration of the nigrostriatal dopaminergic system in Wilson's disease investigated with [123I]ss-CIT and high-resolution SPET

International Nuclear Information System (INIS)

Barthel, H.; Sorger, D.; Kluge, R.; Kuehn, H.-J.; Wagner, A.; Hermann, W.

2001-01-01

Wilson's disease (WD) is a copper deposition disorder which can result in a number of extrapyramidal motoric symptoms such as parkinsonism. Therefore, this study was carried out to investigate, for the first time, nigrostriatal dopaminergic function in WD in relation to different courses and severity of the disease. Using high-resolution single-photon emission tomography (SPET) after administration of 2ss-carbomethoxy-3ss-(4[ 123 I]iodophenyl)tropane ([ 123 I]ss-CIT), striatal dopamine transporters (DAT) were imaged in 43 WD patients and a control group of ten subjects. From the SPET images, specific [ 123 I]ss-CIT binding ratios were obtained for the caudate heads, putamina and entire corpus striatum. In addition, to evaluate a putative dissociation between the caudate and putaminal [ 123 I]ss-CIT binding ratios, the ratio between these binding ratios was calculated (CA/PU ratio). The SPET data were compared with clinical data on the course of the disease (CD), the severity of neurological symptoms and the degree of hepatic alteration. Whereas the specific regional [ 123 I]ss-CIT binding ratios in patients with asymptomatic/hepatic CD did not differ from those in the control group (e.g. striatal ratios: 13.4±3.0 vs 11.7±2.8), in patients with neurological CD the ratios were significantly reduced for all striatal substructures (P=0.003 after one-factor ANOVA). For the different subgroups a tendency was detected towards a stepwise decrease in the specific [ 123 I]ss-CIT binding ratios from pseudo-sclerosis CD (9.4±2.3), through pseudo-parkinsonian CD (9.1±2.1) to arrhythmic-hyperkinetic CD (8.5±1.6). However, these group differences reached significance only for the comparison with asymptomatic/hepatic CD (P=0.02). The CA/PU ratio was significantly higher in WD than in the control group (1.30±0.19 vs 1.11±0.08; P=0.003). Severity of neurological symptoms was significantly correlated with all specific regional [ 123 I]ss-CIT binding ratios (r=-0.49 to -0

Performance Analysis of HF Band FB-MC-SS

Energy Technology Data Exchange (ETDEWEB)

Hussein Moradi; Stephen Andrew Laraway; Behrouz Farhang-Boroujeny

2016-01-01

Abstract—In a recent paper [1] the filter bank multicarrier spread spectrum (FB-MC-SS) waveform was proposed for wideband spread spectrum HF communications. A significant benefit of this waveform is robustness against narrow and partial band interference. Simulation results in [1] demonstrated good performance in a wideband HF channel over a wide range of conditions. In this paper we present a theoretical analysis of the bit error probably for this system. Our analysis tailors the results from [2] where BER performance was analyzed for maximum ration combining systems that accounted for correlation between subcarriers and channel estimation error. Equations are give for BER that closely match the simulated performance in most situations.

Probing the Conformation of an IgG1 Monoclonal Antibody in Lyophilized Solids Using Solid-State Hydrogen-Deuterium Exchange with Mass Spectrometric Analysis (ssHDX-MS).

Science.gov (United States)

Moussa, Ehab M; Singh, Satish K; Kimmel, Michael; Nema, Sandeep; Topp, Elizabeth M

2018-02-05

Therapeutic proteins are often formulated as lyophilized products to improve their stability and prolong shelf life. The stability of proteins in the solid-state has been correlated with preservation of native higher order structure and/or molecular mobility in the solid matrix, with varying success. In the studies reported here, we used solid-state hydrogen-deuterium exchange with mass spectrometric analysis (ssHDX-MS) to study the conformation of an IgG1 monoclonal antibody (mAb) in lyophilized solids and related the extent of ssHDX to aggregation during storage in the solid phase. The results demonstrate that the extent of ssHDX correlated better with aggregation rate during storage than did solid-state Fourier-transform infrared (ssFTIR) spectroscopic measurements. Interestingly, adding histidine to sucrose at different formulation pH conditions decreased aggregation of the mAb, an effect that did not correlate with structural or conformational changes as measured by ssFTIR or ssHDX-MS. Moreover, peptide-level ssHDX-MS analysis in four selected formulations demonstrated global changes across the structure of the mAb when lyophilized with sucrose, trehalose, or mannitol, whereas site-specific changes were observed when lyophilized with histidine as the sole excipient.

78 FR 47056 - Proposed Collection; Comment Request for Forms 941, 941-PR, 941-SS, 941-X, 941-X(PR), Schedule B...

Science.gov (United States)

2013-08-02

... 941, 941-PR, 941- SS, 941-X, 941-X(PR), Schedule B (Form 941), Schedule R (Form 941)and Schedule B (Form 941-PR) AGENCY: Internal Revenue Service (IRS), Treasury. ACTION: Notice and request for comments... Forms 941 (Employer's Quarterly Federal Tax Return), 941-PR, 941-SS (Employer's Quarterly Federal Tax...

Brasiliocroton : a new crotonoid genus of Euphorbiaceae s.s. from eastern Brazil

Science.gov (United States)

Paul E. Berry; Ines Cordeiro; Alex C. Wiedenhoeft; Maria Amelia Vitorino-Cruz; Leticia Ribes de Lima

2005-01-01

A new genus and species of Euphorbiaceae s.s., Brasiliocroton mamoninha, is described from two disjunct areas of lowland forest remnants in eastern and northeastern Brazil. It is a member of tribe Crotoneae and was previously confused with Croton and Micrandra. The resemblance to Micrandra is based on the branched inflorescences and terminal position of the pistillate...

Changes in orientation and appearance of the 100 m arc s radio structure in SS433

Energy Technology Data Exchange (ETDEWEB)

Schilizzi, R T [Netherlands Foundation for Radio Astronomy, Radio Sterrenwacht, Dwingeloo; Miley, G K [Rijksuniversiteit Leiden (Netherlands). Sterrewacht; Romney, J D [Max-Planck-Institut fuer Radioastronomie, Bonn (Germany, F.R.); Spencer, R E [Nuffield Radio Astronomy Labs., Jodrell Bank (UK)

1981-03-26

Results are presented of observations of SS433 made with the European VLBI network for periods of 9 and 10 h at a wavelength of 6 cm on 9 January (JD2444248) and 1-2 June (JD2444392.5) 1980. The results, together with earlier ones of Walker et al., show that the compact radio structure of SS433 changes orientation in a manner consistent with the 164-day precession period inferred from the optical emission lines, but with a phase delay of 5 +- 4 days. The shape of the radio structure is elongated, non-linear and changes from one observing epoch to the other.

Beginning of the End: The Leadership of SS Obersturmbannfuehrer Jochen Peiper

Science.gov (United States)

2004-06-17

of Rudolf Lehmann and Ralf Tiemann on the Leibstandarte are based on the Bundesarchiven (National Archives) in Koblenz and Freiburg in Germany...Hans Schmidt, Paul Hausser, Richard Schulze-Kossens, Rudolf Lehmann, and Ralf Tiemann. Some German sources are at odds with American sources, but that...or a constitution. Among the men who set up the first military training for the Waffen-SS were Felix Steiner and Cassius Freiherr (Baron) von

Pregnancy outcome in patients with sickle cell disease in the UK--a national cohort study comparing sickle cell anaemia (HbSS) with HbSC disease.

Science.gov (United States)

Oteng-Ntim, Eugene; Ayensah, Benjamin; Knight, Marian; Howard, Jo

2015-04-01

We describe the findings from a national study of maternal and fetal outcomes of pregnancy in women with sickle cell disease (SCD). Data were collected via the United Kingdom Obstetric Surveillance System between 1 February 2010 and 31 January 2011 from 109 women, of whom 51 (46·8%) had HbSS and 44 (40·4%) had HbSC. Data included antenatal, maternal and fetal outcomes. Comparisons were made between women with HbSS and HbSC. Incidence of complications were acute pain (57%), blood transfusion (26%), urinary tract infection (UTI; 12%) and critical care unit admission (23%) and these were all more common in women with HbSS than HbSC. There was no difference in the incidence of acute chest syndrome, hypertension and venous thromboembolism between HbSS and HbSC. Women with HbSS were more likely to deliver at pregnancy. © 2014 John Wiley & Sons Ltd.

Search for Doppler-shifted gamma-ray emission from SS 433 using the SMM spectrometer

International Nuclear Information System (INIS)

Geldzahler, B.J.; Share, G.H.; Kinzer, R.L.; Magura, J.; Chupp, E.L.

1989-01-01

Data accumulated from 1980 to 1983 with the Gamma Ray Spectrometer aboard NASA's Solar Maximum Mission (SMM) satellite were searched for evidence of red and blue Doppler-shifted 1.37 MeV Mg-24 nuclear lines from SS 433. The SMM data base covers 270 days when SS 433 was in the field of view and includes periods of radio flaring and quiescence. No evidence was found for Doppler-shifted line emission in any of the spectra. The range of 3-sigma upper limits for individual 9 day integration periods was 0.0008-0.0023 photons/sq cm per sec for the blue beam, encompassing the reported about 1.5 MeV line, and 0.0008-0.002 photons/sq cm per sec for the red beam, encompassing the reported about 1.2 MeV line; the average 3-sigma upper limit in each beam for shifted about 1.37 MeV lines is 0.0015 photons/sq cm per sec for single 9 day integrations. The 3-sigma upper limit on 1.37 MeV gamma-ray emission over 23 9-day integration intervals for the red beam and 28 intervals for the blue beam is 0.0002 photons/sq cm per sec. These new limits from SMM can be reconciled with the HEAO 3 results only if SS 433 emits gamma radiation at or above the SMM sensitivity limit on rare occasions due to variable physical conditions in the system. 19 refs

Search for Doppler-shifted gamma-ray emission from SS 433 using the SMM spectrometer

Science.gov (United States)

Geldzahler, B. J.; Share, G. H.; Kinzer, R. L.; Magura, J.; Chupp, E. L.

1989-01-01

Data accumulated from 1980 to 1983 with the Gamma Ray Spectrometer aboard NASA's Solar Maximum Mission (SMM) satellite were searched for evidence of red and blue Doppler-shifted 1.37 MeV Mg-24 nuclear lines from SS 433. The SMM data base covers 270 days when SS 433 was in the field of view and includes periods of radio flaring and quiescence. No evidence was found for Doppler-shifted line emission in any of the spectra. The range of 3-sigma upper limits for individual 9 day integration periods was 0.0008-0.0023 photons/sq cm per sec for the blue beam, encompassing the reported about 1.5 MeV line, and 0.0008-0.002 photons/sq cm per sec for the red beam, encompassing the reported about 1.2 MeV line; the average 3-sigma upper limit in each beam for shifted about 1.37 MeV lines is 0.0015 photons/sq cm per sec for single 9 day integrations. The 3-sigma upper limit on 1.37 MeV gamma-ray emission over 23 9-day integration intervals for the red beam and 28 intervals for the blue beam is 0.0002 photons/sq cm per sec. These new limits from SMM can be reconciled with the HEAO 3 results only if SS 433 emits gamma radiation at or above the SMM sensitivity limit on rare occasions due to variable physical conditions in the system.

Exploring optimization parameters to increase ssDNA recombineering in Lactococcus lactis and Lactobacillus reuteri.

Science.gov (United States)

Van Pijkeren, Jan-Peter; Neoh, Kar Mun; Sirias, Denise; Findley, Anthony S; Britton, Robert A

2012-01-01

Single-stranded DNA (ssDNA) recombineering is a technology which is used to make subtle changes in the chromosome of several bacterial genera. Cells which express a single-stranded DNA binding protein (RecT or Bet) are transformed with an oligonucleotide which is incorporated via an annealing and replication-dependent mechanism. By in silico analysis we identified ssDNA binding protein homologs in the genus Lactobacillus and Lactococcus lactis. To assess whether we could further improve the recombineering efficiency in Lactobacillus reuteri ATCC PTA 6475 we expressed several RecT homologs in this strain. RecT derived from Enterococcus faecalis CRMEN 19 yielded comparable efficiencies compared with a native RecT protein, but none of the other proteins further increased the recombineering efficiency. We successfully improved recombineering efficiency 10-fold in L. lactis by increasing oligonucleotide concentration combined with the use of oligonucleotides containing phosphorothioate-linkages (PTOs). Surprisingly, neither increased oligonucleotide concentration nor PTO linkages enhanced recombineering in L. reuteri 6475. To emphasize the utility of this technology in improving probiotic features we modified six bases in a transcriptional regulatory element region of the pdu-operon of L. reuteri 6475, yielding a 3-fold increase in the production of the antimicrobial compound reuterin. Directed genetic modification of lactic acid bacteria through ssDNA recombineering will simplify strain improvement in a way that, when mutating a single base, is genetically indistinguishable from strains obtained through directed evolution.

Stress corrosion cracking of 316 SS and Incoloy-800 in high temperature aqueous containing sulfate and chloride

International Nuclear Information System (INIS)

Zhang Weiguo; Lin Fangliang; Gao Fengqin; Zhou Hongyi; Cao Xiaoning

1992-03-01

The stress corrosion cracking (SCC) susceptibility of 316 stainless steel (SS) which was welded for primary pipe and Incoloy-800 (shot peening) for steam generator (SG) tube have been investigated by a slow strain rate test (SSRT) at a strain rate of 4.2 x 10 -6 /s. Tests were conducted at 315 C degree for 316 SS and 270 C degree for In-800 in the oxygenated simulated resin intrusion environment (acidic sulfate). Tests of the effect of combination of SO 4 2- and Cl - on SCC of Incoloy-800 were also carried out. The results indicate that Incoloy-800 is unsusceptible to SCC either in the environment with SO 4 2- (from a few ppm to 1000 ppm, pH 3 ∼ 4) or in the environment of combination of SO 4 2- (1000 ppm) and Cl - (from 2 to 1000 ppm). The 316 NG SS is susceptible to transgranular stress corrosion cracking (TGSCC) in the resin intrusion environment with SO 4 2- in high temperature water

Evaluation of stress-corrosion cracking of sensitized 304SS in low-temperature borated water

International Nuclear Information System (INIS)

Jones, R.H.; Johnson, A.B. Jr.; Bruemmer, S.M.

1981-05-01

Intergranular stress corrosion cracking has been observed in constant extension rate tests, CERT and constant load tests of 304SS tested at 32 0 C in borated water plus 15 ppM C1 - . Evidence of IGSCC was obtained in CERT tests of welded pipe samples only when the original inner diameter surface was intact and with 15 ppM C1 - added to the borated water while IGSCC occurred in a furnace sensitized pipe sample after 500 h at a constant stress of 340 MPa in borated water containing 15 ppM C1 - . These results indicate that surface features associated with weld preparation grinding contributed to the susceptibility of sensitized 304SS to IGSCC in low temperature borated water; however, the constant load test indicates that such surface defects are not necessary for IGSCC in low temperature borated water

The KIzSS network, a sentinel surveillance system for infectious diseases in day care centers: study protocol

Directory of Open Access Journals (Sweden)

Enserink Remko

2012-10-01

Full Text Available Abstract Background Day care-associated infectious diseases are widely recognized as a public health problem but rarely studied. Insights into their dynamics and their association with the day care setting are important for effective decision making in management of infectious disease control. This paper describes the purpose, design and potential of our national multi-center, day care-based sentinel surveillance network for infectious diseases (the KIzSS network. The aim of the KIzSS network is to acquire a long-term insight into the syndromic and microbiological aspects of day care-related infectious diseases and associated disease burden and to model these aspects with day care setting characteristics. Methods/design The KIzSS network applies a prospective cohort design, following day care centers rather than individual children or staff members over time. Data on infectious disease symptoms and related morbidity (children and staff, medical consumption, absenteeism and circulating enteric pathogens (children are collected on a daily, weekly or monthly basis. Every two years, a survey is performed to assess the characteristics of participating day care centers. Discussion The KIzSS network offers a unique potential to study infectious disease dynamics in the day care setting over a sustained period of time. The created (biodatabases will help us to assess day care-related disease burden of infectious diseases among attending children and staff and their relation with the day care setting. This will support the much needed development of evidence-based and pragmatic guidelines for infectious disease control in day care centers.

Chaboche-based cyclic material hardening models for 316 SS–316 SS weld under in-air and pressurized water reactor water conditions

Energy Technology Data Exchange (ETDEWEB)

Mohanty, Subhasish, E-mail: smohanty@anl.gov; Soppet, William K.; Majumdar, Saurindranath; Natesan, Krishnamurti

2016-08-15

Highlights: • 316 SS–316 SS weld cyclically harden/soften while undergoing fatigue loading. • Cyclic hardening/softening creates cycle dependent stress-strain curves. • This necessitate to estimate the cycle dependence of material properties. • Cyclic evolution of Chaboche parameters are estimated under different conditions. - Abstract: This paper discusses a material hardening models for welds made from 316 stainless steel (SS) to 316 SS. The model parameters were estimated from the strain-versus-stress curves obtained from tensile and fatigue tests conducted under different conditions (air at room temperature, air at 300 °C, and primary loop water conditions for a pressurized water reactor). These data were used to check the fatigue cycle dependency of the material hardening parameters (yield stress, parameters related to Chaboche-based linear and nonlinear kinematic hardening models, etc.). The details of the experimental results, material hardening models, and associated calculated results are published in an Argonne report (ANL/LWRS-15/2). This paper summarizes the reported material parameters for 316 SS–316 SS welds and their dependency on fatigue cycles and other test conditions.

Guanxi-verkostojen merkitys kiinalaisessa liiketoimintaympäristössä

OpenAIRE

Hirvonen, Anne; Matveinen, Tarja

2009-01-01

Kiina muuttuu valtavaa vauhtia. Entinen suljettu valtio on kasvanut jättimahdiksi ja yhtäjak-soinen talouskasvu on jatkunut yhdeksän prosentin vuositasolla vuodesta 1979 lähtien. Ulko-maisia yrityksiä kiinnostavat Kiinan halvat työvoimakustannukset ja valtavat markkinat, mutta kiinalaiseen liiketoimintaympäristöön sopeutuminen vaatii yrityksiltä aikaa, kärsivällisyyttä ja oikeanlaisia suhteita. Vaikka ulkoiset muutokset ovat tapahtuneet häkellyttävän nopeasti, vaikuttavat ihmisten sisimmässä ...

Cross-cultural adaptation of the Chilean version of the Voice Symptom Scale - VoiSS.

Science.gov (United States)

Ruston, Francisco Contreras; Moreti, Felipe; Vivero, Martín; Malebran, Celina; Behlau, Mara

This research aims to accomplish the cross-cultural equivalence of the Chilean version of the VoiSS protocol through its cultural and linguistic adaptation. After the translation of the VoiSS protocol to Chilean Spanish by two bilingual speech therapists and its back translation to English, we compared the items of the original tool with the previous translated version. The existing discrepancies were modified by a consensus committee of five speech therapists and the translated version was entitled Escala de Sintomas Vocales - ESV, with 30 questions and five answers: "Never", "Occasionally", "Sometimes", "Most of the time", "Always". For cross-cultural equivalence, the protocol was applied to 15 individuals with vocal problems. In each question the option of "Not applicable" was added to the answer choices for identification of the questions not comprehended or not appropriate for the target population. Two individuals had difficulty answering two questions, which made it necessary to adapt the translation of only one of them. The modified ESV was applied to three individuals with vocal problems, and there were incomprehensible inappropriate questions for the Chilean culture. The ESV reflects the original English version, both in the number of questions and the limitations of the emotional and physical domains. There is now a cross-cultural equivalence of VoiSS in Chilean Spanish, titled ESV. The validation of the ESV for Chilean Spanish is ongoing. RESUMEN Este estudio tuvo como objetivo realizar la equivalencia cultural de la versión Chilena del protocolo Voice Symptom Scale - VoiSS por medio de su adaptación cultural y lingüística. Después de la traducción del VoiSS para el Español Chileno, por dos fonoaudiólogos bilingües, y de la retro traducción para el inglés, se realizó una comparación de los ítems del instrumento original con la versión traducida, surgiendo discrepancias; tales divergencias fueron resueltas por un comité compuesto por

Omaa ääntä etsimässä : syntetisaattorin käyttö Orbit-yhtyeessä

OpenAIRE

Myrskog, Mikael

2014-01-01

Musiikkikappaleessa on mielestäni oleellisinta sen sisäinen, esteettinen maailma ja se tunne, joka tästä välittyy. Tässä kokonaisuudessa yksi tärkeimmistä tekijöistä on mielestäni kappaleen eri instrumenttien luoma yhteinen saundimaailma. Opinnäytetyössäni tutkin ja kuvailen, miten käytän syntetisaattoreita saundien luomiseen fuusiojazzyhtyeessä Orbit. Mielestäni on tärkeää olla tietoinen siitä musiikin perinteestä, minkä jatkumon osana itse on. Näin esittelen ja analysoin kappaleita,...

Protein dynamics during presynaptic complex assembly on individual ssDNA molecules

OpenAIRE

Gibb, Bryan; Ye, Ling F.; Kwon, YoungHo; Niu, Hengyao; Sung, Patrick; Greene, Eric C.

2014-01-01

Homologous recombination is a conserved pathway for repairing double?stranded breaks, which are processed to yield single?stranded DNA overhangs that serve as platforms for presynaptic complex assembly. Here we use single?molecule imaging to reveal the interplay between Saccharomyce cerevisiae RPA, Rad52, and Rad51 during presynaptic complex assembly. We show that Rad52 binds RPA?ssDNA and suppresses RPA turnover, highlighting an unanticipated regulatory influence on protein dynamics. Rad51 b...

Optimization of the diffusion bonding parameters for SS316L/CuCrZr with and without Nickel interlayer

Energy Technology Data Exchange (ETDEWEB)

Singh, K.P., E-mail: kpsingh@ipr.res.in; Patel, Alpesh; Bhope, Kedar; Khirwadkar, S.S.; Mehta, Mayur

2016-11-15

Highlights: • Optimization of diffusion bonding parameters for dissimilar materials SS316L with CuCrZr is proposed with and without Nickel interlayer. • Ultrasonic testing on diffusion bonded samples provides the overall information/signatures about well-bonded and de-bonded samples. • Microstructural examination confirms the diffusion of Nickel into parent materials. • Mechanical characterization such as hardness measurement and shear measurement supports the results. - Abstract: Divertor & Firstwall module of ITER like tokamak has several joints of dissimilar materials such as Tungsten (W) to Copper (Cu), Cu to CuCrZr (heat sink material) and CuCrZr to Stainless Steel (SS) materials. These Plasma Facing Components (PFC) are made of multi-layered joints, which are to be exposed to the harsh environment of Plasma with the expected heat flux of 5–10 MW/m{sup 2}. The joining of SS316L material to CuCrZr material is proposed in this paper. As SS316L/CuCrZr being dissimilar materials, direct joining of these materials is a problem, which needs a suitable fabrication process. The joining of SS316L with heat sink material (CuCrZr) requires the good thermal transfer and sound in structural joint. Diffusion bonding technique has been adopted as a process for joining of these two dissimilar materials. The primary objective of the joining study is to obtain the best diffusion bonding parameters for ITER like tokamak application. Thermo-mechanical simulator machine (Gleeble 3800) is used to perform diffusion bonding experiments at different temperatures 650 °C, 850 °C, 900 °C, 950 °C & 1000 °C, different uniaxial pressure varying from 5 MPa to 15 MPa and with the holding time of 15 min & 30 min, using pure nickel interlayer. The diffusion bonded samples have undergone Non-destructive testing (NDT) particularly the ultrasonic examination using immersion probe technique, microstructural examination by the High Resolution (HR) electron microscopy (SEM), Energy

Optimization of the diffusion bonding parameters for SS316L/CuCrZr with and without Nickel interlayer

International Nuclear Information System (INIS)

Singh, K.P.; Patel, Alpesh; Bhope, Kedar; Khirwadkar, S.S.; Mehta, Mayur

2016-01-01

Highlights: • Optimization of diffusion bonding parameters for dissimilar materials SS316L with CuCrZr is proposed with and without Nickel interlayer. • Ultrasonic testing on diffusion bonded samples provides the overall information/signatures about well-bonded and de-bonded samples. • Microstructural examination confirms the diffusion of Nickel into parent materials. • Mechanical characterization such as hardness measurement and shear measurement supports the results. - Abstract: Divertor & Firstwall module of ITER like tokamak has several joints of dissimilar materials such as Tungsten (W) to Copper (Cu), Cu to CuCrZr (heat sink material) and CuCrZr to Stainless Steel (SS) materials. These Plasma Facing Components (PFC) are made of multi-layered joints, which are to be exposed to the harsh environment of Plasma with the expected heat flux of 5–10 MW/m 2 . The joining of SS316L material to CuCrZr material is proposed in this paper. As SS316L/CuCrZr being dissimilar materials, direct joining of these materials is a problem, which needs a suitable fabrication process. The joining of SS316L with heat sink material (CuCrZr) requires the good thermal transfer and sound in structural joint. Diffusion bonding technique has been adopted as a process for joining of these two dissimilar materials. The primary objective of the joining study is to obtain the best diffusion bonding parameters for ITER like tokamak application. Thermo-mechanical simulator machine (Gleeble 3800) is used to perform diffusion bonding experiments at different temperatures 650 °C, 850 °C, 900 °C, 950 °C & 1000 °C, different uniaxial pressure varying from 5 MPa to 15 MPa and with the holding time of 15 min & 30 min, using pure nickel interlayer. The diffusion bonded samples have undergone Non-destructive testing (NDT) particularly the ultrasonic examination using immersion probe technique, microstructural examination by the High Resolution (HR) electron microscopy (SEM), Energy

Structural insights of the ssDNA binding site in the multifunctional endonuclease AtBFN2 from Arabidopsis thaliana.

Directory of Open Access Journals (Sweden)

Tsung-Fu Yu

Full Text Available The multi S1/P1 nuclease AtBFN2 (EC 3.1.30.1 encoded by the Arabidopsis thaliana At1g68290 gene is a glycoprotein that digests RNA, ssDNA, and dsDNA. AtBFN2 depends on three zinc ions for cleaving DNA and RNA at 3'-OH to yield 5'-nucleotides. In addition, AtBFN2's enzymatic activity is strongly glycan dependent. Plant Zn(2+-dependent endonucleases present a unique fold, and belong to the Phospholipase C (PLC/P1 nuclease superfamily. In this work, we present the first complete, ligand-free, AtBFN2 crystal structure, along with sulfate, phosphate and ssDNA co-crystal structures. With these, we were able to provide better insight into the glycan structure and possible enzymatic mechanism. In comparison with other nucleases, the AtBFN2/ligand-free and AtBFN2/PO4 models suggest a similar, previously proposed, catalytic mechanism. Our data also confirm that the phosphate and vanadate can inhibit the enzyme activity by occupying the active site. More importantly, the AtBFN2/A5T structure reveals a novel and conserved secondary binding site, which seems to be important for plant Zn(2+-dependent endonucleases. Based on these findings, we propose a rational ssDNA binding model, in which the ssDNA wraps itself around the protein and the attached surface glycan, in turn, reinforces the binding complex.

X-ray variability of SS 433: Evidence for supercritical accretion

Science.gov (United States)

Atapin, K. E.; Fabrika, S. N.

2016-08-01

We study the X-ray variability of SS 433 based on data from the ASCA observatory and the MAXI and RXTE/ASM monitoring missions. Based on the ASCA data, we have constructed the power spectrum of SS 433 in the frequency range from 10-6 to 0.1 Hz, which confirms the presence of a flat portion in the spectrum at frequencies 3 × 10-5-10-3 Hz. The periodic variability (precession, nutation, eclipses) begins to dominate significantly over the stochastic variability at lower frequencies, which does not allow the stochastic variability to be studied reliably. The model in which the flat portion extends to 9.5 × 10-6 Hz, while a power-law rise with an index of 2.6 occurs below provides the best agreement with the observations. The nutational oscillations of the jets with a period of about three days suggests that the time for the passage of material through the disk is less than this value. At frequencies below 4 × 10-6 Hz, the shape of the power spectrum probably does not reflect the disk structure but is determined by external factors, for example, by a change in the amount of material supplied by the donor. The flat portion can arise from a rapid decrease in the viscous time in the supercritical or radiative disk zones. The flat spectrum is associated with the variability of the X-ray jets that are formed in the supercritical disk region.

Possible reduction of hepatoma formation by Smmu 7721 cells in SCID mice and metastasis formation by B16F10 melanoma cells in C57BL/6 mice by Agaricus blazei murill extract.

Science.gov (United States)

Wu, Ming-Fang; Lu, Hsu-Feng; Hsu, Yu-Ming; Tang, Ming-Chu; Chen, Hsueh-Chin; Lee, Ching-Sung; Yang, Yi-Yuan; Yeh, Ming-Yang; Chung, Hsiung-Kwang; Huang, Yi-Ping; Wu, Chih-Chung; Chung, Jing-Gung

2011-01-01

Agaricus blazei Murill extract (ABM) has been reported to possess antitumor effects. In this study, the role of ABM in tumor growth and metastasis in vivo was evaluated in experimental Smmu 7721 hepatoma cells in severe combined immunodeficiency (SCID) mice and B16F10 melanoma cells lung metastasis in C57BL/6 mice. For the tumor growth model, the size of the liver tumor mass was about 10 mm to 20 mm in the control group. In comparison with the control group, the tumor mass seem to grow slowly with ABM treatment, especially at the high dose. For the tumor metastasis model, after a six-week treatment, the survival rates of B6 mice were 0%, 30%, 10% and 50% for control group, low, median and high concentration ABM treatment groups, respectively. The survival rate showed that pretreatment of C57BL/6 (B6) mice with ABM lengthened their lifespan after tumor cell inoculation, which supports the notion that ABM successfully reduced lung metastasis formation by B16F10 melanoma cells. The treatment effect was dependent on the concentration of ABM for tumor growth and metastasis in these models.

Scale Model Acoustic Test Validation of IOP-SS Water Prediction using Loci-STREAM-VoF

Science.gov (United States)

Nielsen, Tanner; West, Jeff

2015-01-01

The Scale Model Acoustic Test (SMAT) is a 5% scale test of the Space Launch System (SLS), which is currently being designed at Marshall Space Flight Center (MSFC). SMAT consists of a 5% scale representation of the ignition overpressure sound-suppression system (IOP-SS) that is being tested to quantify the water flow and induced air entrainment in and around the mobile launcher exhaust hole. This data will be compared with computational fluid dynamics (CFD) simulations using the newly developed Loci-STREAM Volume of Fluid (VoF) methods. Compressible and incompressible VoF methods have been formulated, and are currently being used to simulate the water flow of SMAT IOP-SS. The test data will be used to qualitatively and quantitatively assess and validate the VoF methods.

Melanoma inhibitor of apoptosis protein (ML-IAP) specific cytotoxic T lymphocytes cross-react with an epitope from the auto-antigen SS56

DEFF Research Database (Denmark)

Baek Sørensen, Rikke; Faurschou, Mikkel; Troelsen, Lone

2009-01-01

A large proportion of melanoma patients host a spontaneous T-cell response specifically against ML-IAP-derived peptides. In this study, we describe that some ML-IAP-specific cytotoxic T cells isolated from melanoma patients cross react with an epitope from the auto-antigen SS56. SS56 is a recentl...

Electrical Properties of Al, Ag, Cu, Ti and SS Thin Film for Electrode of Solar Cell

International Nuclear Information System (INIS)

Bambang Siswanto; Wirjoadi; Sudjatmoko; Tjipto Sujitno

2003-01-01

The Al, Ag, Cu, Ti and SS materials were deposited on the surface of glass substrate using plasma DC sputtering technique. The deposition process was done with the following plasma parameters : deposition time, gas pressure and substrate temperature with the aim to obtain a good conductance of thin films. Variation of substrate deposition time was 1 - 15 minutes, gas pressure was 5x10 -2 - 7x10 -2 torr and of temperature was 100 - 300 o C. The resistance measurement has been done by four points probes and the conductivity was calculated using mathematic formulation. It was obtained that the minimum resistance in the order of R = 0.07 Ω, was found at Ag materials and this was obtained at the following plasma parameters : deposition time 15 minutes, gas pressure 6x10 -2 torr and temperature 300 o C, while, the resistance of : Cu, Al, Ti and SS materials were R = 0.13 Ω, R = 450 Ω, R = 633 Ω, R = 911 Ω respectively, It could be concluded that the Ag thin film has a minimum resistance, high conductivity compared to the other materials Al, Cu, Ti and SS. Ag is therefore the suitable material for applying as electrode of solar cell. (author)

The complex, variable structure of stationary lines in SS433

International Nuclear Information System (INIS)

Falomo, R.; Boksenberg, A.; Tanzi, E.G.; Tarenghi, M.; Treves, A.

1987-01-01

On 1979 June 3-6, a number of spectra of SS433 were obtained using the UCL Image Photon Counting System on the 3.6-m telescope of the European Southern Observatory, La Silla, Chile. The stationary Hα and He I lambdalambda5875, 6678 and 7065 lines have a complex structure which on June 4-5 exhibited a central feature accompanied by two equally displaced (+- 1000 km s -1 ) side components. Variability of the line profile and equivalent width is observed on time-scales as short as a quarter of an hour. (author)

Tribological changes on SS304 stainless steel induced by nitrogen plasma immersion ion implantation with and without auxiliary heating

International Nuclear Information System (INIS)

Mello, C.B.; Ueda, M.; Lepienski, C.M.; Reuther, H.

2009-01-01

In order to achieve quite thick treated layers with reasonable thickness uniformity in SS304 steel, the plasma immersion ion implantation (PIII) process was run in high-temperature, up to 350 deg. C, to induce high thermal diffusion but avoid the white layer formation. In these experiments, we heated the sample-holder with a shielded resistive wire properly wound around it and subjected the SS samples to nitrogen glow discharge PIII with relatively low voltages (10 kV) in different temperatures. We also treated the SS samples by the traditional PIII method, slowly increasing the high voltage pulse intensities, until 14 kV at the end of processing, reaching temperatures of up to 350 deg. C. These modes of treatments were compared with respect to nitrogen implantation profiles, X-ray diffraction, tribology and mechanical properties. X-ray diffraction results indicated a much higher efficiency of auxiliary heated PIII mode compared to the ordinary PIII. Very prominent γ N peaks were observed for the first mode, indicating large concentration of nitrogen in thick layers, confirmed by the nitrogen profiles measured by GDOS and AES. Improved mechanical and tribological properties were obtained for SS304 samples treated by the PIII with auxiliary heating, more than for ordinary PIII. Hardness was enhanced by up to 2.77 times, as seen by nanoindentation tests.

Polymorphisms rs12998 and rs5780218 in KiSS1 Suppressor Metastasis Gene in Mexican Patients with Breast Cancer

Directory of Open Access Journals (Sweden)

Edhit Guadalupe Cruz Quevedo

2015-01-01

Full Text Available Aims. KiSS1 is a metastasis suppressor gene associated with inhibition of cellular chemotaxis and invasion attenuating the metastasis in melanoma and breast cancer cell lines. Along the KiSS-1 gene at least 294 SNPs have been described; however the association of these polymorphisms as genetic markers for metastasis in breast cancer studies has not been investigated. Here we describe two simple PCR-RFLPs protocols to identify the rs5780218 (9DelT and the rs12998 (E20K KiSS1 polymorphisms and the allelic, genotypic, and haplotypic frequencies in Mexican general population (GP and patients with benign breast disease (BBD or breast cancer (BC. Results. The rs5780218 polymorphism was individually associated with breast cancer (P=0.0332 and the rs12998 polymorphism shows statistically significant differences when GP versus case (BC and BBD groups were compared (P<0.0001. The H1 Haplotype (G/- occurred more frequently in BC group (0.4256 whereas H2 haplotype (G/T was the most prevalent in BBD group (0.4674. Conclusions. Our data indicated that the rs5780218 polymorphism individually confers susceptibility for development of breast cancer in Mexican population and a possible role as a genetic marker in breast cancer metastasis for H1 haplotype (Wt/variant in KiSS1 gene must be analyzed in other populations.

CADRE-SS, an in Silico Tool for Predicting Skin Sensitization Potential Based on Modeling of Molecular Interactions.

Science.gov (United States)

Kostal, Jakub; Voutchkova-Kostal, Adelina

2016-01-19

Using computer models to accurately predict toxicity outcomes is considered to be a major challenge. However, state-of-the-art computational chemistry techniques can now be incorporated in predictive models, supported by advances in mechanistic toxicology and the exponential growth of computing resources witnessed over the past decade. The CADRE (Computer-Aided Discovery and REdesign) platform relies on quantum-mechanical modeling of molecular interactions that represent key biochemical triggers in toxicity pathways. Here, we present an external validation exercise for CADRE-SS, a variant developed to predict the skin sensitization potential of commercial chemicals. CADRE-SS is a hybrid model that evaluates skin permeability using Monte Carlo simulations, assigns reactive centers in a molecule and possible biotransformations via expert rules, and determines reactivity with skin proteins via quantum-mechanical modeling. The results were promising with an overall very good concordance of 93% between experimental and predicted values. Comparison to performance metrics yielded by other tools available for this endpoint suggests that CADRE-SS offers distinct advantages for first-round screenings of chemicals and could be used as an in silico alternative to animal tests where permissible by legislative programs.

Specific binding component of the 'inactive' stereoisomer (S,S)-[125I]IQNB to rat brain muscarinic receptors in vivo

International Nuclear Information System (INIS)

Boulay, Sheila F.; McRee, R. Carter; Cohen, Victor I.; Sood, Virendar K.; Zeeberg, Barry R.; Reba, Richard C.

1996-01-01

In vivo nonspecific binding can be estimated using the inactive stereoisomer of a receptor radioligand. However, the binding of the inactive stereoisomer may be partially specific. Specific binding of the inactive (S,S)-[ 125 I]IQNB was estimated from the inhibition induced by a competing nonradioactive ligand. This technique differed from the usual approach, since it was used to study the inactive rather than the active stereoisomer. The results indicate that there is substantial specific binding for (S,S)-[ 125 I]IQNB

Efficient Programming for Multicore Processor Heterogeneity: OpenMP versus OmpSs

OpenAIRE

Butko , Anastasiia; Bruguier , Florent; Gamatié , Abdoulaye; Sassatelli , Gilles

2017-01-01

International audience; ARM single-ISA heterogeneous multicore processors combine high-performance big cores with power-efficient small cores. They aim at achieving a suitable balance between performance and energy. How- ever, a main challenge is to program such architectures so as to efficiently exploit their features. In this paper, we study the impact on performance and energy trade-offs of single-ISA architecture according to OpenMP 3.0 and the OmpSs programming models. We consider differ...

ssDNA Pairing Accuracy Increases When Abasic Sites Divide Nucleotides into Small Groups.

Directory of Open Access Journals (Sweden)

Alexandra Peacock-Villada

Full Text Available Accurate sequence dependent pairing of single-stranded DNA (ssDNA molecules plays an important role in gene chips, DNA origami, and polymerase chain reactions. In many assays accurate pairing depends on mismatched sequences melting at lower temperatures than matched sequences; however, for sequences longer than ~10 nucleotides, single mismatches and correct matches have melting temperature differences of less than 3°C. We demonstrate that appropriately grouping of 35 bases in ssDNA using abasic sites increases the difference between the melting temperature of correct bases and the melting temperature of mismatched base pairings. Importantly, in the presence of appropriately spaced abasic sites mismatches near one end of a long dsDNA destabilize the annealing at the other end much more effectively than in systems without the abasic sites, suggesting that the dsDNA melts more uniformly in the presence of appropriately spaced abasic sites. In sum, the presence of appropriately spaced abasic sites allows temperature to more accurately discriminate correct base pairings from incorrect ones.

Characterization, Corrosion Resistance, and Cell Response of High-Velocity Flame-Sprayed HA and HA/TiO2 Coatings on 316L SS

Science.gov (United States)

Singh, Tejinder Pal; Singh, Harpreet; Singh, Hazoor

2012-09-01

The main aim of this study is to evaluate corrosion and biocompatibility behavior of thermal spray hydroxyapatite (HA) and hydroxyapatite/titania bond (HA/TiO2)-coated 316L stainless steel (316L SS). In HA/TiO2 coatings, TiO2 was used as a bond coat between HA top coat and 316L SS substrate. The coatings were characterized by x-ray diffraction and scanning electron microscopy/energy dispersive spectroscopy, and corrosion resistance determined for the uncoated substrate and the two coatings. The biological behavior was investigated by the cell culture studies using osteosarcoma cell line KHOS-NP (R-970-5). The corrosion resistance of the steel was found to increase after the deposition of the HA and HA/TiO2 bond coatings. Both HA, as well as, HA/TiO2 coatings exhibit excellent bond strength of 49 and 47 MPa, respectively. The cell culture studies showed that HA-coated 316L SS specimens appeared more biocompatible than the uncoated and HA/TiO2-coated 316L SS specimens.

Caffeine inhibits gene conversion by displacing Rad51 from ssDNA

Science.gov (United States)

Tsabar, Michael; Mason, Jennifer M.; Chan, Yuen-Ling; Bishop, Douglas K.; Haber, James E.

2015-01-01

Efficient repair of chromosomal double-strand breaks (DSBs) by homologous recombination relies on the formation of a Rad51 recombinase filament that forms on single-stranded DNA (ssDNA) created at DSB ends. This filament facilitates the search for a homologous donor sequence and promotes strand invasion. Recently caffeine treatment has been shown to prevent gene targeting in mammalian cells by increasing non-productive Rad51 interactions between the DSB and random regions of the genome. Here we show that caffeine treatment prevents gene conversion in yeast, independently of its inhibition of the Mec1ATR/Tel1ATM-dependent DNA damage response or caffeine's inhibition of 5′ to 3′ resection of DSB ends. Caffeine treatment results in a dosage-dependent eviction of Rad51 from ssDNA. Gene conversion is impaired even at low concentrations of caffeine, where there is no discernible dismantling of the Rad51 filament. Loss of the Rad51 filament integrity is independent of Srs2's Rad51 filament dismantling activity or Rad51's ATPase activity and does not depend on non-specific Rad51 binding to undamaged double-stranded DNA. Caffeine treatment had similar effects on irradiated HeLa cells, promoting loss of previously assembled Rad51 foci. We conclude that caffeine treatment can disrupt gene conversion by disrupting Rad51 filaments. PMID:26019181

Taxonomy and phylogeny of Lopharia s.s., Dendrodontia, Dentocorticium and Fuscocerrena (Basidiomycota, Polyporales)

Science.gov (United States)

Shi-Liang Liu; Karen K. Nakasone; Sheng-Hua Wu; Shuang-Hui He; Yu-Cheng. Dai

2018-01-01

Eleven taxa of Lopharia s.s., Dendrodontia, Dentocorticium and Fuscocerrena in Polyporales are included in the phylogenetic analyses of nuc rDNA ITS1-5.8S-ITS2 (ITS), D1-D2 domains of nuc 28S rDNA (28S) and RNA polymerase II second-largest subunit (rpb2) sequences. New species Lopharia resupinata...

Effect of Thermal Aging and Test Temperatures on Fracture Toughness of SS 316(N) Welds

Science.gov (United States)

Dutt, B. Shashank; Babu, M. Nani; Shanthi, G.; Moitra, A.; Sasikala, G.

2018-03-01

The effect of thermal aging and test temperatures on fracture toughness (J 0.2) of SS 316(N) weld material has been studied based on J-R curve evaluations. The aging of the welds was carried out at temperatures 370, 475 and 550 °C and for durations varying from 1000 to 20,000 h. The fracture toughness (J-R curve) tests were carried out at 380 and 550 °C for specimens after all aging conditions, including as-weld condition. The initiation fracture toughness (J 0.2) of the SS 316(N) weld material has shown degradation after 20,000-h aging durations and is reflected in all the test temperatures and aging temperatures. The fracture toughness after different aging conditions and test temperatures, including as-weld condition, was higher than the minimum specified value for this class of welds.

Hold your horSSEs: controlling structure-selective endonucleases MUS81 and Yen1/GEN1.

Science.gov (United States)

Blanco, Miguel G; Matos, Joao

2015-01-01

Repair of DNA lesions through homologous recombination promotes the establishment of stable chromosomal interactions. Multiple helicases, topoisomerases and structure-selective endonucleases (SSEs) act upon recombining joint molecules (JMs) to disengage chromosomal connections and safeguard chromosome segregation. Recent studies on two conserved SSEs - MUS81 and Yen1/GEN1- uncovered multiple layers of regulation that operate to carefully tailor JM-processing according to specific cellular needs. Temporal restriction of SSE function imposes a hierarchy in pathway usage that ensures efficient JM-processing while minimizing reciprocal exchanges between the recombining DNAs. Whereas a conserved strategy of fine-tuning SSE functions exists in different model systems, the precise molecular mechanisms to implement it appear to be significantly different. Here, we summarize the current knowledge on the cellular switches that are in place to control MUS81 and Yen1/GEN1 functions.

Burnout syndrome among dental students: a short version of the "Burnout Clinical Subtype Questionnaire" adapted for students (BCSQ-12-SS).

Science.gov (United States)

Montero-Marin, Jesus; Monticelli, Francesca; Casas, Marina; Roman, Amparo; Tomas, Inmaculada; Gili, Margarita; Garcia-Campayo, Javier

2011-12-12

Burnout has been traditionally defined in relation to the dimensions of "exhaustion", "cynicism", and "inefficiency". More recently, the Burnout Clinical Subtype Questionnaire (BCSQ-12) further established three different subtypes of burnout: the "frenetic" subtype (related to "overload"), the "under-challenged" subtype (related to "lack of development"), and the "worn-out" subtype (related to "neglect"). However, to date, these definitions have not been applied to students. The aims of this research were (1) to adapt a Spanish version of the BCSQ-12 for use with students, (2) to test its factorial validity, internal consistency, convergent and discriminant validity, and (3) to assess potential socio-demographic and occupational risk factors associated with the development of the subtypes. We used a cross-sectional design on a sample of dental students (n = 314) from Santiago and Huesca universities (Spain). Participants completed the Burnout Clinical Subtype Questionnaire Student Survey (BCSQ-12-SS), the Maslach Burnout Inventory Student Survey (MBI-SS), and a series of socio-demographic and occupational questions formulated for the specific purpose of this study. Data were subjected to exploratory factor analysis (EFA) using the principal component method with varimax orthogonal rotation. To assess the relations with the criterion, we calculated the Pearson correlation coefficient (r), multiple correlation coefficient (R(y.123)), and the coefficient of determination (R(2)(y.123)). To assess the association between the subtypes and the socio-demographic variables, we examined the adjusted odds ratio (OR) obtained from multivariate logistic regression models. Factorial analyses supported the theoretical proposition of the BCSQ-12-SS, with α-values exceeding 0.80 for all dimensions. The "overload-exhaustion" relation was r = 0.59 (p burnout as established by the BCSQ-12-SS. As such, the BCSQ-12-SS can be used for the recognition of clinical profiles and for the

Effect of Low-Temperature Sensitization on the Corrosion Behavior of AISI Type 304L SS Weld Metal in Simulated Groundwater

Science.gov (United States)

Suresh, Girija; Nandakumar, T.; Viswanath, A.

2018-05-01

The manuscript presents the investigations carried out on the effect of low-temperature sensitization (LTS) of 304L SS weld metal on its corrosion behavior in simulated groundwater, for its application as a canister material for long-term storage of nuclear vitrified high-level waste in geological repositories. AISI type 304L SS weld pad was fabricated by multipass gas tungsten arc welding process using 308L SS filler wire. The as-welded specimens were subsequently subjected to carbide nucleation and further to LTS at 500 °C for 11 days to simulate a temperature of 300 °C for 100-year life of the canister in geological repositories. Delta ferrite ( δ-ferrite) content of the 304L SS weld metal substantially decreased on carbide nucleation treatment and further only a marginal decrease occurred on LTS treatment. The microstructure of the as-welded consisted of δ-ferrite as a minor phase distributed in austenite matrix. The δ-ferrite appeared fragmented in the carbide-nucleated and LTS-treated weld metal. The degree of sensitization measured by double-loop electrochemical potentokinetic reactivation method indicated an increase in carbide nucleation treatment when compared to the as-welded specimens, and further increase occurred on LTS treatment. Potentiodynamic anodic polarization investigations in simulated groundwater indicated a substantial decrease in the localized corrosion resistance of the carbide-nucleated and LTS 304L SS weld metals, when compared to the as-welded specimens. Post-experimental micrographs indicated pitting as the primary mode of attack in the as-welded, while pitting and intergranular corrosion (IGC) occurred in the carbide-nucleated weld metal. LTS-treated weld metal predominantly underwent IGC attack. The decrease in the localized corrosion resistance of the weld metal after LTS treatment was found to have a direct correlation with the degree of sensitization and the weld microstructure. The results are detailed in the manuscript.

Effect of Low-Temperature Sensitization on the Corrosion Behavior of AISI Type 304L SS Weld Metal in Simulated Groundwater

Science.gov (United States)

Suresh, Girija; Nandakumar, T.; Viswanath, A.

2018-04-01

The manuscript presents the investigations carried out on the effect of low-temperature sensitization (LTS) of 304L SS weld metal on its corrosion behavior in simulated groundwater, for its application as a canister material for long-term storage of nuclear vitrified high-level waste in geological repositories. AISI type 304L SS weld pad was fabricated by multipass gas tungsten arc welding process using 308L SS filler wire. The as-welded specimens were subsequently subjected to carbide nucleation and further to LTS at 500 °C for 11 days to simulate a temperature of 300 °C for 100-year life of the canister in geological repositories. Delta ferrite (δ-ferrite) content of the 304L SS weld metal substantially decreased on carbide nucleation treatment and further only a marginal decrease occurred on LTS treatment. The microstructure of the as-welded consisted of δ-ferrite as a minor phase distributed in austenite matrix. The δ-ferrite appeared fragmented in the carbide-nucleated and LTS-treated weld metal. The degree of sensitization measured by double-loop electrochemical potentokinetic reactivation method indicated an increase in carbide nucleation treatment when compared to the as-welded specimens, and further increase occurred on LTS treatment. Potentiodynamic anodic polarization investigations in simulated groundwater indicated a substantial decrease in the localized corrosion resistance of the carbide-nucleated and LTS 304L SS weld metals, when compared to the as-welded specimens. Post-experimental micrographs indicated pitting as the primary mode of attack in the as-welded, while pitting and intergranular corrosion (IGC) occurred in the carbide-nucleated weld metal. LTS-treated weld metal predominantly underwent IGC attack. The decrease in the localized corrosion resistance of the weld metal after LTS treatment was found to have a direct correlation with the degree of sensitization and the weld microstructure. The results are detailed in the manuscript.

Photometric behavior of SS 433 in 1979 and 1980

International Nuclear Information System (INIS)

Mazeh, T.; Tel Aviv University; Wise Observatory, Tel Aviv, Israel); Leibowitz, E.M.; Wise Observatory, Tel Aviv, Israel)

1981-01-01

Results and analysis of photometric measurements performed in the last two observing seasons of SS 433 are presented. The light of the star in the V and the B photometric bands varies with a period of either 6.55 or 6.43 days. The periodicity of approximately 164 days is also apparent in the photometric data, with its first and third harmonics. Fluctuations of the order of a half a magnitude within a few hours have also been recorded. The B-V color shows no dependence on the phase of the periodic variations, while it does change by up to 0.25 mag, in an apparent association with the short time scale fluctuations

Butt Weldability for SS400 Using Laser-Arc Hybrid Welding

International Nuclear Information System (INIS)

Kim, Jong Do; Myoung, Gi Hoon; Park, Duck; Myoung, Gi Hoon; Park, In Duck

2016-01-01

This study presents results of an experimental investigation of the laser-arc, hybrid, butt welding process of SS400 structural steel. Welding parameters including laser power, welding current and speed were varied in order to obtain one-pass, full-penetration welds without defects. The conditions that resulted in optimal beads were identified. After welding, hardness measurements and microstructure observations were carried out in order to study weld properties. The mechanical properties of both the base material and welded specimen were compared based on the results of tensile strength measurements. The yield and tensile strengths were found to be similar

Butt Weldability for SS400 Using Laser-Arc Hybrid Welding

Energy Technology Data Exchange (ETDEWEB)

Kim, Jong Do; Myoung, Gi Hoon; Park, Duck [Korea Maritime and Ocean Univ., Busan (Korea, Republic of); Myoung, Gi Hoon; Park, In Duck [Korea Institute of Machinery and Materials, Busan (Korea, Republic of)

2016-07-15

This study presents results of an experimental investigation of the laser-arc, hybrid, butt welding process of SS400 structural steel. Welding parameters including laser power, welding current and speed were varied in order to obtain one-pass, full-penetration welds without defects. The conditions that resulted in optimal beads were identified. After welding, hardness measurements and microstructure observations were carried out in order to study weld properties. The mechanical properties of both the base material and welded specimen were compared based on the results of tensile strength measurements. The yield and tensile strengths were found to be similar.

Volume-dependent K+ transport in rabbit red blood cells comparison with oxygenated human SS cells

Energy Technology Data Exchange (ETDEWEB)

Al-Rohil, N.; Jennings, M.L.

1989-07-01

In this study the volume-dependent or N-ethylmaleimide (NEM)-stimulated, ouabain-insensitive K+ influx and efflux were measured with the tracer 86Rb+ in rabbit red blood cells. The purpose of the work was to examine the rabbit as a potential model for cell volume regulation in human SS red blood cells and also to investigate the relationship between the NEM-reactive sulfhydryl group(s) and the signal by which cell swelling activates the transport. Ouabain-resistant K+ efflux and influx increase nearly threefold in cells swollen hypotonically by 15%. Pretreatment with 2 mM NEM stimulates efflux 5-fold and influx 10-fold (each measured in an isotonic medium). The ouabain-resistant K+ efflux was dependent on the major anion in the medium. The anion dependence of K+ efflux in swollen or NEM-stimulated cells was as follows: Br- greater than Cl- much greater than NO3- = acetate. The magnitudes of both the swelling- and the NEM-stimulated fluxes are much higher in young cells (density separated but excluding reticulocytes) than in older cells. Swelling- or NEM-stimulated K+ efflux in rabbit red blood cells was inhibited 50% by 1 mM furosemide, and the inhibitory potency of furosemide was enhanced by extracellular K+, as is known to be true for human AA and low-K+ sheep red blood cells. The swelling-stimulated flux in both rabbit and human SS cells has a pH optimum at approximately 7.4. We conclude that rabbit red blood cells are a good model for swelling-stimulated K+ transport in human SS cells.

Comparative wood anatomy of Andromedeae s.s., Gaultherieae, Lyonieae and Oxydendreae (Vaccinioideae, Ericaceae s.l.)

NARCIS (Netherlands)

Lens, F.; Smets, E.; Jansen, S.

2004-01-01

The wood anatomical structure of 11 out of 13 genera from four tribes of the Vaccinioideae, namely Andromedeae s.s., Gaultherieae, Lyonieae and Oxydendreae (Ericaceae s.l.), is described using light and scanning electron microscopy. Several features of the secondary xylem support the tribal

Small molecule probes finely differentiate between various ds- and ss-DNA and RNA by fluorescence, CD and NMR response

Energy Technology Data Exchange (ETDEWEB)

Crnolatac, Ivo; Rogan, Iva; Majić, Boris; Tomić, Sanja [Division of Organic Chemistry and Biochemistry, Division of Physical Chemistry, Ruđer Bošković Institute, P.O. Box 180, 10002 Zagreb (Croatia); Deligeorgiev, Todor [Faculty of Chemistry and Pharmacy, University of Sofia (Bulgaria); Horvat, Gordan [Department of Physical Chemistry, Faculty of Science/Chemistry, Horvatovac 102A, HR-10000 Zagreb (Croatia); Makuc, Damjan; Plavec, Janez [Slovenian NMR Centre, National Institute of Chemistry, Hajdrihova 19, Ljubljana (Slovenia); EN-FIST Centre of Excellence, Trg Osvobodilne Fronte 13, Ljubljana (Slovenia); Pescitelli, Gennaro [Department of Chemistry, University of Pisa, Via Moruzzi 13, Pisa (Italy); Piantanida, Ivo, E-mail: pianta@irb.hr [Division of Organic Chemistry and Biochemistry, Division of Physical Chemistry, Ruđer Bošković Institute, P.O. Box 180, 10002 Zagreb (Croatia)

2016-10-12

Two small molecules showed intriguing properties of analytical multipurpose probes, whereby one chromophore gives different signal for many different DNA/RNA by application of several highly sensitive spectroscopic methods. Dyes revealed pronounced fluorescence ratiomeric differentiation between ds-AU-RNA, AT-DNA and GC-DNA in approximate order 10:8:1. Particularly interesting, dyes showed specific fluorimetric response for poly rA even at 10-fold excess of any other ss-RNA, and moreover such emission selectivity is preserved in multicomponent ss-RNA mixtures. The dyes also showed specific chiral recognition of poly rU in respect to the other ss-RNA by induced CD (ICD) pattern in visible range (400–500 nm), which was attributed to the dye-side-chain contribution to binding (confirmed by absence of any ICD band for reference compound lacking side-chain). Most intriguingly, minor difference in the side-chain attached to dye chromophore resulted in opposite sign of dye-ICD pattern, whereby differences in NMR NOESY contacts and proton chemical shifts between two dye/oligo rU complexes combined with MD simulations and CD calculations attributed observed bisignate ICD to the dimeric dye aggregate within oligo rU. - Highlights: • Novel dyes emit fluorescence only for poly rA even at high excess of all other ss-RNA. • Fluorescence response for AT-DNA is 8 times stronger than for GC-DNA. • Florescence induced by ds-RNA is 20% stronger that emission induced by ds-DNA. • Intrinsically non-chiral, dyes show strong and characteristic ICD response for poly rU.

Experimental comparison of pathogenic potential of two sibling species Anisakis simplex s.s. and Anisakis pegreffii in Wistar rat.

Science.gov (United States)

del Carmen Romero, María; Valero, Adela; Navarro-Moll, María Concepción; Martín-Sánchez, Joaquina

2013-08-01

There are little data available on the pathology caused by the sibling species Anisakis simplex s.s. and Anisakis pegreffii. The differences shown in their ability to penetrate the muscle of fish may also be manifested in humans. The purpose of this study is to confirm possible differences in pathogenicity between A. simplex s.s. and A. pegreffii using an experimental model which simulates infection in humans. Female Wistar rats were infected with 190 Anisakis type I L3 larvae from the Iberian coastline. After the animal was sacrificed, these L3 larvae were then recovered and identified via PCR-RFLP of the ITS1-5.8S-ITS2. A logistic regression analysis was performed searching for association between experimental pathogenic potential and species. The distribution of A. simplex s.s. and A. pegreffii between Atlantic and Mediterranean waters of the Iberian Peninsula showed statistically significant differences (P  0.3). 21.6% showed pathogenic potential, interpreted as the capacity of the larvae to cause lesions, stick to the gastrointestinal wall or penetrate it. The species variable showed association with the pathogenic role of the larva (P = 0.008). Taking A. simplex s.s. as our reference, the OR for A. pegreffii is 0.351 (P = 0.028). Despite this difference, A. pegreffii is also capable of causing anisakiasis, being responsible for 14.3% of the penetrations of the gastric mucosa found in rats, which justifies both species being considered aetiologic agents of this parasitic disorder. © 2013 John Wiley & Sons Ltd.

Läsnäolo ja yleisösuhde : mitä tapahtuu ohjelmatyössä ja ilmaisussa kun tekniikka muuttuu?

OpenAIRE

Baer, Titta

2013-01-01

Yhden lähtökohdan ja inspiraation tälle työlle antoi mediatutkija Marshall McLuhanin tunnettu lause "väline on viesti". Opinnäytetyössä tarkastellaan, miten teknologian kehitys - kamerat, tietotekniikka - näkyy esimerkiksi TV:n ajankohtaisohjelmien ja uutisten tuotannossa. Työssä selvitetään, miten välineiden tekninen kehitys vaikuttaa, miten tekniikka muuttaa TV-ohjelmien rakennetta, ilmettä ja yleisösuhdetta. Käytetyt menetelmät ovat laadullisia: kirjallisuutta, tutkimuksia ja haastatteluja...

Reduced infectivity of waterborne viable but nonculturable Helicobacter pylori strain SS1 in mice.

Science.gov (United States)

Boehnke, Kevin F; Eaton, Kathryn A; Fontaine, Clinton; Brewster, Rebecca; Wu, Jianfeng; Eisenberg, Joseph N S; Valdivieso, Manuel; Baker, Laurence H; Xi, Chuanwu

2017-08-01

Helicobacter pylori infection has been consistently associated with lack of access to clean water and proper sanitation, but no studies have demonstrated that the transmission of viable but nonculturable (VBNC) H. pylori can occur from drinking contaminated water. In this study, we used a laboratory mouse model to test whether waterborne VBNCH. pylori could cause gastric infection. We performed five mouse experiments to assess the infectivity of VBNCH. pylori in various exposure scenarios. VBNC viability was examined using Live/Dead staining and Biolog phenotype metabolism arrays. High doses of VBNCH. pylori in water were chosen to test the "worst-case" scenario for different periods of time. One experiment also investigated the infectious capabilities of VBNC SS1 using gavage. Further, immunocompromised mice were exposed to examine infectivity among potentially vulnerable groups. After exposure, mice were euthanized and their stomachs were examined for H. pylori infection using culture and PCR methodology. VBNC cells were membrane intact and retained metabolic activity. Mice exposed to VBNCH. pylori via drinking water and gavage were not infected, despite the various exposure scenarios (immunocompromised, high doses) that might have permitted infection with VBNCH. pylori. The positive controls exposed to viable, culturable H. pylori did become infected. While other studies that have used viable, culturable SS1 via gavage or drinking water exposures to successfully infect mice, in our study, waterborne VBNC SS1 failed to colonize mice under all test conditions. Future studies could examine different H. pylori strains in similar exposure scenarios to compare the relative infectivity of the VBNC vs the viable, culturable state, which would help inform future risk assessments of H. pylori in water. © 2017 The Authors. Helicobacter Published by John Wiley & Sons Ltd.

Kehonkuvaa eheyttämässä - kehonkuva psykofyysisessä fysioterapiassa

OpenAIRE

Härkälä, Emilia

2014-01-01

Kuntoutuslainsäädännön mukaan fysioterapia kuuluu lääkinnälliseen kuntoutukseen, jonka tavoitteena on vaikuttaa ihmisen toimintakykyyn ja normaalin elämän edellytyksiin. Käytännössä ihmisen toimintakykyä arvioidaan sen mukaan, millaiset fyysiset, psyykkiset ja sosiaaliset edellytykset yksilöllä on selviytyä päivittäisistä toiminnoista ja askareista, kuten työstä, opiskelusta, sosiaalisista suhteista ja vapaa-ajan toimista. Toimintakyky on siis laaja käsite, ja tähän kuuluvat oleellisesti fyys...

Arsenic-Redox Transformation and Plant Growth Promotion by Purple Nonsulfur Bacteria Rhodopseudomonas palustris CS2 and Rhodopseudomonas faecalis SS5.

Science.gov (United States)

Batool, Kanza; Tuz Zahra, Fatima; Rehman, Yasir

2017-01-01

Arsenic (As) is a well-known toxic metalloid found naturally and released by different industries, especially in developing countries. Purple nonsulfur bacteria (PNSB) are known for wastewater treatment and plant growth promoting abilities. As-resistant PNSB were isolated from a fish pond. Based on As-resistance and plant growth promoting attributes, 2 isolates CS2 and SS5 were selected and identified as Rhodopseudomonas palustris and Rhodopseudomonas faecalis , respectively, through 16S rRNA gene sequencing. Maximum As(V) resistance shown by R. faecalis SS5 and R. palustris CS2 was up to 150 and 100 mM, respectively. R . palustris CS2 showed highest As(V) reduction up to 62.9% (6.29 ± 0.24 mM), while R. faecalis SS5 showed maximum As(III) oxidation up to 96% (4.8 ± 0.32 mM), respectively. Highest auxin production was observed by R. palustris CS2 and R. faecalis SS, up to 77.18 ± 3.7 and 76.67 ± 2.8  μ g mL -1 , respectively. Effects of these PNSB were tested on the growth of Vigna mungo plants. A statistically significant increase in growth was observed in plants inoculated with isolates compared to uninoculated plants, both in presence and in absence of As. R. palustris CS2 treated plants showed 17% (28.1 ± 0.87 cm) increase in shoot length and 21.7% (7.07 ± 0.42 cm) increase in root length, whereas R. faecalis SS5 treated plants showed 12.8% (27.09 ± 0.81 cm) increase in shoot length and 18.8% (6.9 ± 0.34 cm) increase in root length as compared to the control plants. In presence of As, R. palustris CS2 increased shoot length up to 26.3% (21.0 ± 1.1 cm), while root length increased up to 31.3% (5.3 ± 0.4 cm), whereas R. faecalis SS5 inoculated plants showed 25% (20.7 ± 1.4 cm) increase in shoot length and 33.3% (5.4 ± 0.65 cm) increase in root length as compared to the control plants. Bacteria with such diverse abilities could be ideal for plant growth promotion in As-contaminated sites.

V 1343 aquilae (SS 433) as a double-periodic star

International Nuclear Information System (INIS)

Goranskij, V.P.

1983-01-01

The new computer method of double periodicity search earlier tested on the Blazhko effect in RR Lyrae type variable stars is applied to define more precise periods of brightness variability of the binary V 1343 Aql (SS 433). Computer program was used in the two-parameter search regime. The obtained periods are P 1 = 13sup(d).074+-0sup(d).008 and P 2 = 163sup(d).8+-1sup(d).2. The periodically repeating brithness curve deeps treated as primary minima (the accretion disc eclipsed by the star) vary their shape with the phase of period P 2 . The expected eclipse at 1979 October 16 did nor occur

Implementation of QoSS (Quality-of-Security Service) for NoC-Based SoC Protection

Science.gov (United States)

Sepúlveda, Johanna; Pires, Ricardo; Strum, Marius; Chau, Wang Jiang

Many of the current electronic systems embedded in a SoC (System-on-Chip) are used to capture, store, manipulate and access critical data, as well as to perform other key functions. In such a scenario, security is considered as an important issue. The Network-on-chip (NoC), as the foreseen communication structure of next-generation SoC devices, can be used to efficiently incorporate security. Our work proposes the implementation of QoSS (Quality of Security Service) to overcome present SoC vulnerabilities. QoSS is a novel concept for data protection that introduces security as a dimension of QoS. In this paper, we present the implementation of two security services (access control and authentication), that may be configured to assume one from several possible levels, the implementation of a technique to avoid denial-of-service (DoS) attacks, evaluate their effectiveness and estimate their impact on NoC performance.

Synthesis of carbon nanotube using camphor with SS 316 as catalytic substrate via oxidative heat treatment preparation method

Science.gov (United States)

Wulan, Praswasti Pembangun Dyah Kencana; Angelina, Dian

2017-11-01

Carbon nanotube (CNT) is a material that now often become the topic in nanotechnology research. CNT is widely used in the electronics industry especially for TV and computer flat panel displays, devices, automotives for car components, and batteries. Also for defense industries as well as other industries such as sports equipment. Camphor (C10H16O), a botanical hydrocarbon, can be used as a renewable and low cost carbon source for CNT synthesis. Synthesis was performed with stainless steel-316 (SS 316) as substrate, argon as carrier gas, and hydrogen as co-reactant. Preparation of the SS 316 was through a pretreatment by oxidative heat treatment method at a temperature of 850oC for 30 minutes, to remove the layer of chrome and make a rough surface as a growth media for CNT. The operating temperature of the synthesis used was 800oC with a reaction time of 60 minutes. Reactor, which made from stainless steel 316 (SS 316), was used for synthesis CNTs with maximum camphor mass of 20 grams. This research was conducted by varying the number of camphor mass by 5, 7, 10, 12, and 15 grams. The results showed that camphor decomposed into three compounds which are 40% benzene, 8% toluene, and 52% xylene. CNT grows on the surface of the SS 316 plate for each variation. CNTs have grown by follow tips growth model with deformations like buckling growth model and continuous growth model were also founded. The results of XRD showed that CNT were found in every camphor mass variation with high intensity at 2θ angle of 26° and 43°. The best quality and yield of CNT was obtained at camphor mass of 15 grams with carbon percentage of 87,1% and diameter 33 - 44 nm.

Ferulic Acid, But Not All Hydroxycinnamic Acids, Is a Novel T3SS Inducer of Ralstonia solanacearum and Promotes Its Infection Process in Host Plants under Hydroponic Condition

Directory of Open Access Journals (Sweden)

Yong Zhang

2017-09-01

Full Text Available Hydroxycinnamic acids (HCAs are typical monocyclic phenylpropanoids, including cinnamic acid (Cin, coumaric acid (Cou, caffeic acid (Caf, ferulic acid (FA and their isomers, and involved in the interactions between pathogens and host plants. Here, we focused on the impact of HCAs on expression of type III secretion system (T3SS in Ralstonia solanacearum. FA significantly induced the expression of the T3SS and some type III effectors (T3Es genes in hrp-inducing medium, while did not the other HCAs. However, exogenously supplemented FA did not affect the T3SS expression in planta and the elicitation of the hypersensitive response (HR in tobacco leaves. Consistent with its central roles in pathogenicity, the FA-induced expression of the T3SS led to significant promotion on infection process of R. solanacearum in tomato plants under hydroponics cultivation. Moreover, the FA-induced expression of the T3SS was specifically mediated by the well-characterized signaling cascade PrhA-prhI/R-PrhJ-HrpG-HrpB, independent of the other known regulatory pathways. In summary, our results demonstrated that FA, a novel inducer of the T3SS in R. solanacearum, was able to promote its infection process in host plants under hydroponics condition.

Integral test of KERMA data for SS304 stainless steel in the D-T fusion neutron environment

International Nuclear Information System (INIS)

Ikeda, Y.; Kosako, K.; Konno, C.

1994-01-01

The KERMA (Kinetic Energy Release Material) data play the fundamental role for estimating nuclear heating in the structural components of fusion reactors. The data are produced from the large body of nuclear data relevant to reaction channels associated with the kinetic energy release. Both contributions by neutron and gamma-ray should be addressed to arrived at the final heating products. Extensive efforts have been devoted to the neutron and γ-ray transport profile in many materials, resulting in the validation of cross section data. However, the experimental verification of KERMA data, which is a highly integrated product of neutron and γ-ray, has been limited from the lack of available experimental data. Through the JAERI/USDOE collaborative program on fusion neutronics, novel experimental technique for the direct nuclear heating due to 14 MeV neutrons has been developed based on a micro calorimetric system. The technique demonstrated excellent capability for detecting the temperature rise due to nuclear heating and pertinent verification for the calculation data and methods. This paper deals with the most recent experimental endeavor for the direct nuclear heating measurement in SS-304 stainless steel assembly, where appreciably large amounts of slow neutron and associated secondary γ-rays dominated the field. The nuclear heating up to 200 mm depth in the SS-304 assembly were derived from detected temperature rise employing large SS-304 block type probe materials

Age-dependent oxidation of extracellular cysteine/cystine redox state (Eh(Cys/CySS)) in mouse lung fibroblasts is mediated by a decline in Slc7a11 expression.

Science.gov (United States)

Zheng, Yuxuan; Ritzenthaler, Jeffrey D; Burke, Tom J; Otero, Javier; Roman, Jesse; Watson, Walter H

2018-04-01

Aging is associated with progressive oxidation of the extracellular environment. The redox state of human plasma, defined by the concentrations of cysteine (Cys) and cystine (CySS), becomes more oxidized as we age. Recently, we showed that fibroblasts isolated from the lungs of young and old mice retain this differential phenotype; old cells produce and maintain a more oxidizing extracellular redox potential (E h (Cys/CySS)) than young cells. Microarray analysis identified down-regulation of Slc7a11, the light subunit of the CySS/glutamate transporter, as a potential mediator of age-related oxidation in these cells. The purpose of the present study was to investigate the mechanistic link between Slc7a11 expression and extracellular E h (Cys/CySS). Sulforaphane treatment or overexpression of Slc7a11 was used to increase Slc7a11 in lung fibroblasts from old mice, and sulfasalazine treatment or siRNA-mediated knock down was used to decrease Slc7a11 in young fibroblasts. Slc7a11 mRNA levels were measured by real-time PCR, Slc7a11 activity was determined by measuring the rate of glutamate release, Cys, CySS, glutathione (GSH) and its disulfide (GSSG) were measured by HPLC, and E h (Cys/CySS) was calculated from the Nernst equation. The results showed that both E h (Cys/CySS) and E h (GSH/GSSG) were more oxidized in the conditioned media of old cells than in young cells. Up-regulation of Slc7a11 via overexpression or sulforaphane treatment restored extracellular E h (Cys/CySS) in cultures of old cells, whereas down-regulation reproduced the oxidizing E h (Cys/CySS) in young cells. Only sulforaphane treatment was able to increase total GSH and restore E h (GSH/GSSG), whereas overexpression, knock down and sulfasalazine had no effect on these parameters. In addition, inhibition of GSH synthesis with buthionine sulfoximine had no effect on the ability of cells to restore their extracellular redox potential in response to an oxidative challenge. In conclusion, our study

Thick SS316 materials TIG welding development activities towards advanced fusion reactor vacuum vessel applications

Science.gov (United States)

Kumar, B. Ramesh; Gangradey, R.

2012-11-01

Advanced fusion reactors like ITER and up coming Indian DEMO devices are having challenges in terms of their materials design and fabrication procedures. The operation of these devices is having various loads like structural, thermo-mechanical and neutron irradiation effects on major systems like vacuum vessel, divertor, magnets and blanket modules. The concept of double wall vacuum vessel (VV) is proposed in view of protecting of major reactor subsystems like super conducting magnets, diagnostic systems and other critical components from high energy 14 MeV neutrons generated from fusion plasma produced by D-T reactions. The double walled vacuum vessel is used in combination with pressurized water circulation and some special grade borated steel blocks to shield these high energy neutrons effectively. The fabrication of sub components in VV are mainly used with high thickness SS materials in range of 20 mm- 60 mm of various grades based on the required protocols. The structural components of double wall vacuum vessel uses various parts like shields, ribs, shells and diagnostic vacuum ports. These components are to be developed with various welding techniques like TIG welding, Narrow gap TIG welding, Laser welding, Hybrid TIG laser welding, Electron beam welding based on requirement. In the present paper the samples of 20 mm and 40 mm thick SS 316 materials are developed with TIG welding process and their mechanical properties characterization with Tensile, Bend tests and Impact tests are carried out. In addition Vickers hardness tests and microstructural properties of Base metal, Heat Affected Zone (HAZ) and Weld Zone are done. TIG welding application with high thick SS materials in connection with vacuum vessel requirements and involved criticalities towards welding process are highlighted.

Thick SS316 materials TIG welding development activities towards advanced fusion reactor vacuum vessel applications

International Nuclear Information System (INIS)

Kumar, B Ramesh; Gangradey, R

2012-01-01

Advanced fusion reactors like ITER and up coming Indian DEMO devices are having challenges in terms of their materials design and fabrication procedures. The operation of these devices is having various loads like structural, thermo-mechanical and neutron irradiation effects on major systems like vacuum vessel, divertor, magnets and blanket modules. The concept of double wall vacuum vessel (VV) is proposed in view of protecting of major reactor subsystems like super conducting magnets, diagnostic systems and other critical components from high energy 14 MeV neutrons generated from fusion plasma produced by D-T reactions. The double walled vacuum vessel is used in combination with pressurized water circulation and some special grade borated steel blocks to shield these high energy neutrons effectively. The fabrication of sub components in VV are mainly used with high thickness SS materials in range of 20 mm- 60 mm of various grades based on the required protocols. The structural components of double wall vacuum vessel uses various parts like shields, ribs, shells and diagnostic vacuum ports. These components are to be developed with various welding techniques like TIG welding, Narrow gap TIG welding, Laser welding, Hybrid TIG laser welding, Electron beam welding based on requirement. In the present paper the samples of 20 mm and 40 mm thick SS 316 materials are developed with TIG welding process and their mechanical properties characterization with Tensile, Bend tests and Impact tests are carried out. In addition Vickers hardness tests and microstructural properties of Base metal, Heat Affected Zone (HAZ) and Weld Zone are done. TIG welding application with high thick SS materials in connection with vacuum vessel requirements and involved criticalities towards welding process are highlighted.

Proton transfer and hydrogen bonding in the organic solid state: a combined XRD/XPS/ssNMR study of 17 organic acid-base complexes.

Science.gov (United States)

Stevens, Joanna S; Byard, Stephen J; Seaton, Colin C; Sadiq, Ghazala; Davey, Roger J; Schroeder, Sven L M

2014-01-21

The properties of nitrogen centres acting either as hydrogen-bond or Brønsted acceptors in solid molecular acid-base complexes have been probed by N 1s X-ray photoelectron spectroscopy (XPS) as well as (15)N solid-state nuclear magnetic resonance (ssNMR) spectroscopy and are interpreted with reference to local crystallographic structure information provided by X-ray diffraction (XRD). We have previously shown that the strong chemical shift of the N 1s binding energy associated with the protonation of nitrogen centres unequivocally distinguishes protonated (salt) from hydrogen-bonded (co-crystal) nitrogen species. This result is further supported by significant ssNMR shifts to low frequency, which occur with proton transfer from the acid to the base component. Generally, only minor chemical shifts occur upon co-crystal formation, unless a strong hydrogen bond is formed. CASTEP density functional theory (DFT) calculations of (15)N ssNMR isotropic chemical shifts correlate well with the experimental data, confirming that computational predictions of H-bond strengths and associated ssNMR chemical shifts allow the identification of salt and co-crystal structures (NMR crystallography). The excellent agreement between the conclusions drawn by XPS and the combined CASTEP/ssNMR investigations opens up a reliable avenue for local structure characterization in molecular systems even in the absence of crystal structure information, for example for non-crystalline or amorphous matter. The range of 17 different systems investigated in this study demonstrates the generic nature of this approach, which will be applicable to many other molecular materials in organic, physical, and materials chemistry.

Discovery of the double Doppler-shifted emission-line systems in the X-ray spectrum of SS 433

Science.gov (United States)

Kotani, Taro; Kawai, Nobuyuki; Aoki, Takashi; Doty, John; Matsuoka, Masaru; Mitsuda, Kazuhisa; Nagase, Fumiaki; Ricker, George; White, Nick E.

1994-01-01

We have used the CCD X-ray spectrometers on ASCA and resolved the X-ray emission line from the jet of SS 433 both into Doppler-shifted components with two distinct velocities, and into emission from different ionization states of iron, i.e., Fe XXV and Fe XXVI. This is the first direct detection of the two Doppler shifted beams in the X-ray spectra of SS 433 and allows the radial velocity of the jet along the line of sight to be determined with an accuracy comparable to the optical spectroscopy. We also found pairs of emission lines from other atomic species, such as ionized silicon and sulfur, with the Doppler shifts consistent with each other. This confirms the origin of the X-ray emission in the high temperature plasma in the jets.

Parameter Optimization Of Natural Hydroxyapatite/SS316l Via Metal Injection Molding (MIM)

Science.gov (United States)

Mustafa, N.; Ibrahim1, M. H. I.; Amin, A. M.; Asmawi, R.

2017-01-01

Metal injection molding (MIM) are well known as a worldwide application of powder injection molding (PIM) where as applied the shaping concept and the beneficial of plastic injection molding but develops the applications to various high performance metals and alloys, plus metal matrix composites and ceramics. This study investigates the strength of green part by using stainless steel 316L/ Natural hydroxyapatite composite as a feedstock. Stainless steel 316L (SS316L) was mixed with Natural hydroxyapatite (NHAP) by adding 40 wt. % Low Density Polyethylene and 60 %wt. Palm Stearin as a binder system at 63 wt. % powder loading consist of 90 % wt. of SS316 L and 10 wt. % NHAP prepared thru critical powder volume percentage (CPVC). Taguchi method was functional as a tool in determining the optimum green strength for Metal Injection Molding (MIM) parameters. The green strength was optimized with 4 significant injection parameter such as Injection temperature (A), Mold temperature (B), Pressure (C) and Speed (D) were selected throughout screening process. An orthogonal array of L9 (3)4 was conducted. The optimum injection parameters for highest green strength were established at A1, B2, C0 and D1 and where as calculated based on Signal to Noise Ratio.

Multi-institutional comparison of simulated treatment delivery errors in ssIMRT, manually planned VMAT and autoplan-VMAT plans for nasopharyngeal radiotherapy

DEFF Research Database (Denmark)

Pogson, Elise M; Aruguman, Sankar; Hansen, Christian R

2017-01-01

PURPOSE: To quantify the impact of simulated errors for nasopharynx radiotherapy across multiple institutions and planning techniques (auto-plan generated Volumetric Modulated Arc Therapy (ap-VMAT), manually planned VMAT (mp-VMAT) and manually planned step and shoot Intensity Modulated Radiation...... Therapy (mp-ssIMRT)). METHODS: Ten patients were retrospectively planned with VMAT according to three institution's protocols. Within one institution two further treatment plans were generated using differing treatment planning techniques. This resulted in mp-ssIMRT, mp-VMAT, and ap-VMAT plans. Introduced...

Performance analysis of SS7 congestion controls under sustained overload

Science.gov (United States)

Manfield, David R.; Millsteed, Gregory K.; Zukerman, Moshe

1994-04-01

Congestion controls are a key factor in achieving the robust performance required of common channel signaling (CCS) networks in the face of partial network failures and extreme traffic loads, especially as networks become large and carry high traffic volume. The CCITT recommendations define a number of types of congestion control, and the parameters of the controls must be well set in order to ensure their efficacy under transient and sustained signalling network overload. The objective of this paper is to present a modeling approach to the determination of the network parameters that govern the performance of the SS7 congestion controls under sustained overload. Results of the investigation by simulation are presented and discussed.

Možnosti přenosu signalizace SS7 přes IP síť s využitím ústředny YATE

OpenAIRE

Al-Anqari, Mhannad

2013-01-01

Diplomová Práce se věnuje problematikou signalizačního systému číslo 7 (SS7), a to zejména přenosem signalizace SS7 přes sítě na bázi IP protokolu. K ověření možností přenosu byla vytvořena konvergovaná síť s využitím Open Source PBX YATE, kde jsou potřebné protokoly implementovány. V úvodu diplomové práce je uveden popis signalizačního systému SS7, který je následován vysvětlením funkce každé z vrstev (MTP2 až aplikační) a přenášených zpráv síti SS7. Dále byla věnována pozornost protokolům, ...

NExSS/NAI Joint ExoPAG SAG 16 Report on Remote Biosignatures for Exoplanets

Science.gov (United States)

Kiang, Nancy Y.; Parenteau, Mary Nicole; Domagal-Goldman, Shawn

2017-01-01

Future exoplanet observations will soon focus on the search for life beyond the Solar System. Exoplanet biosignatures to be sought are those with global, potentially detectable, impacts on a planet. Biosignatures occur in an environmental context in which geological, atmospheric, and stellar processes and interactions may work to enhance, suppress or mimic these biosignatures. Thus biosignature scienceis inherently interdisciplinary. Its advance is necessary to inform the design of the next flagship missions that will obtain spectra of habitable extrasolar planets. The NExSS NAI Joint Exoplanet Biosignatures Workshop Without Walls brought together the astrobiology, exoplanet, and mission concept communities to review, discuss, debate, and advance the science of remote detection of planetary biosignatures. The multi-meeting workshop began in June 2016, and was a process that engaged a broad range of experts across the interdisciplinary reaches of NASA's Nexus for Exoplanet System Science (NExSS) program, the NASA Astrobiology Institute (NAI), NASAs Exoplanet Exploration Program (ExEP), and international partners, such as the European Astrobiology Network Association (EANA) and Japans Earth Life Science Institute (ELSI). These groups spanned expertise in astronomy, planetary science, Earth sciences, heliophysics, biology, instrument mission development, and engineering.

Sol–gel derived solar selective coatings on SS 321 substrates for solar thermal applications

Energy Technology Data Exchange (ETDEWEB)

Subasri, R., E-mail: subasri@arci.res.in; Soma Raju, K.R.C.; Reddy, D.S.; Hebalkar, Neha Y.; Padmanabham, G.

2016-01-01

Sol–gel derived multilayered solar selective coatings were generated on AISI SS 321 substrates using Ag-TiO{sub 2} as the cermet layer, titania and silica as the dielectric layers with high and low refractive indices respectively. The phase compositions of the individual layers were independently confirmed using grazing angle incidence X-ray diffraction, which was corroborated by X-ray photoelectron spectroscopic analysis. Thickness of the layers was measured using variable angle spectroscopic ellipsometry. The solar absorbance was measured over the UV–Vis-NIR wavelength range. Thermal emissivity was determined using FTIR spectroscopic analysis. The durability of the coatings was ascertained using accelerated corrosion testing methods as well as by measuring the optical properties after thermal cycling experiments. The promising nature of hexavalent chrome-free, environmental friendly, multilayered solar selective coating was ascertained with respect to amenability to scale-up. - Highlights: • Sol–gel derived multilayered solar selective coatings developed on SS321 • Solar absorptance and thermal emittance at par with toxic chrome coating • Thermal stability and corrosion resistance of coatings studied • Coating performance found to be promising for large scale applications • Scale-up amenability investigated by coating generation on 1 m tubes.

Sol–gel derived solar selective coatings on SS 321 substrates for solar thermal applications

International Nuclear Information System (INIS)

Subasri, R.; Soma Raju, K.R.C.; Reddy, D.S.; Hebalkar, Neha Y.; Padmanabham, G.

2016-01-01

Sol–gel derived multilayered solar selective coatings were generated on AISI SS 321 substrates using Ag-TiO_2 as the cermet layer, titania and silica as the dielectric layers with high and low refractive indices respectively. The phase compositions of the individual layers were independently confirmed using grazing angle incidence X-ray diffraction, which was corroborated by X-ray photoelectron spectroscopic analysis. Thickness of the layers was measured using variable angle spectroscopic ellipsometry. The solar absorbance was measured over the UV–Vis-NIR wavelength range. Thermal emissivity was determined using FTIR spectroscopic analysis. The durability of the coatings was ascertained using accelerated corrosion testing methods as well as by measuring the optical properties after thermal cycling experiments. The promising nature of hexavalent chrome-free, environmental friendly, multilayered solar selective coating was ascertained with respect to amenability to scale-up. - Highlights: • Sol–gel derived multilayered solar selective coatings developed on SS321 • Solar absorptance and thermal emittance at par with toxic chrome coating • Thermal stability and corrosion resistance of coatings studied • Coating performance found to be promising for large scale applications • Scale-up amenability investigated by coating generation on 1 m tubes

Proposed real-time data processing system to control source and special nuclear material (SS) at Mound Laboratory

International Nuclear Information System (INIS)

DeVer, E.A.; Baston, M.; Bishop, T.C.

1976-01-01

The SS Acountability System was designed to provide accountability of all SS materials by unit identification and grams. The existing system is a gram-accountable system. The new system was designed to incorporate unit identification into an ADP (Automated Data Processing) System. It also records all transactions performed against a particular unit of accountable material. The high volume of data is input via CRT terminals. Input data will consist of the following: source of the material (its unit identification), amount of material being moved, isotopic content, type of material, Health Physics number of the person moving the material, account number from which the material is being moved, unit identification of the material being moved (if all material is not moved), Health Physics number of the person receiving the material, account number to which material is being moved, and acceptance of the material by the receiver. A running inventory of all material is kept. At the end of the month the physical inventory will be compared to the data base and all discrepancies reported. Since a complete history of transactions has been kept, the source and cause for any discrepancies should be easily located. Discrepancies are held to a minimum since errors are detected before entrance into the data base. The system will also furnish all reports necessary to control SS Accountability. These reports may be requested at any time via an accountability master terminal

Novel ssDNA Viruses Detected in the Virome of Bleached, Habitat-Forming Kelp Ecklonia radiata

Directory of Open Access Journals (Sweden)

Douglas T. Beattie

2018-01-01

Full Text Available Kelp forests provide essential habitats for organisms in temperate rocky shores. Loss of kelp forests has occurred over large areas in a number of temperate regions, including in Australia, where the dominant kelp Ecklonia radiata has been lost from substantial areas of the shoreline. Loss of E. radiata has been associated with environmental stressors, including increased temperature and anthropogenic contaminants, as well as biological factors, such as herbivory. Disease may also play a role, but there is little information on the role of disease in the loss of kelp from coastal ecosystems or on the potential role of pathogenic microorganisms, such as viruses. E. radiata across much of its distribution in Australia can develop a “bleached” phenotype, which may be a disease. To investigate whether the phenotype was associated with a potential viral agent, we shotgun sequenced viral particles that were isolated from kelp with normal (healthy and bleached phenotypes. Each virome consisted of ~380,000 reads, of which ~25% were similar to known viruses. All samples were dominated by bacteriophages, but novel ssDNA virus sequences were detected that were almost exclusively in viromes from the bleached kelp phenotype. These ssDNA viruses are covered by 11 contigs that contained complete capsids and characteristic rep genes that were 30–60% similar to those of circular, Rep-encoding ssDNA viruses (CRESS-DNA viruses. CRESS-DNA viruses have not previously been described from macroalgae, and the rep genes were similar to CRESS-DNA viruses from marine water samples, snails, crabs, anemones, but also dragonflies. This raises the interesting possibility that the kelp could be a vector of the CRESS-DNA viruses to other organisms that are associated with the bleached state.

Validation of the early childhood attitude toward women in science scale (ECWiSS): A pilot administration

Science.gov (United States)

Mulkey, Lynn M.

The intention of this research was to measure attitudes of young children toward women scientists. A 27-item instrument, the Early Childhood Women in Science Scale (ECWiSS) was validated in a test case of the proposition that differential socialization predicts entry into the scientific talent pool. Estimates of internal consistency indicated that the scale is highly reliable. Known groups and correlates procedures, employed to determine the validity of the instrument, revealed that the scale is able to discriminate significant differences between groups and distinguishes three dimensions of attitude (role-specific self-concept, home-related sex-role conflict, and work-related sex-role conflict). Results of the analyses also confirmed the anticipated pattern of correlations with measures of another construct. The findings suggest the utility of the ECWiSS for measurement of early childhood attitudes in models of the ascriptive and/or meritocratic processes affecting recruitment to science and more generally in program and curriculum evaluation where attitude toward women in science is the construct of interest.

Easi-CRISPR for creating knock-in and conditional knockout mouse models using long ssDNA donors.

Science.gov (United States)

Miura, Hiromi; Quadros, Rolen M; Gurumurthy, Channabasavaiah B; Ohtsuka, Masato

2018-01-01

CRISPR/Cas9-based genome editing can easily generate knockout mouse models by disrupting the gene sequence, but its efficiency for creating models that require either insertion of exogenous DNA (knock-in) or replacement of genomic segments is very poor. The majority of mouse models used in research involve knock-in (reporters or recombinases) or gene replacement (e.g., conditional knockout alleles containing exons flanked by LoxP sites). A few methods for creating such models have been reported that use double-stranded DNA as donors, but their efficiency is typically 1-10% and therefore not suitable for routine use. We recently demonstrated that long single-stranded DNAs (ssDNAs) serve as very efficient donors, both for insertion and for gene replacement. We call this method efficient additions with ssDNA inserts-CRISPR (Easi-CRISPR) because it is a highly efficient technology (efficiency is typically 30-60% and reaches as high as 100% in some cases). The protocol takes ∼2 months to generate the founder mice.

Genome-wide association mapping including phenotypes from relatives without genotypes in a single-step (ssGWAS for 6-week body weight in broiler chickens

Directory of Open Access Journals (Sweden)

Huiyu eWang

2014-05-01

Full Text Available The purpose of this study was to compare results obtained from various methodologies for genome-wide association studies, when applied to real data, in terms of number and commonality of regions identified and their genetic variance explained, computational speed, and possible pitfalls in interpretations of results. Methodologies include: two iteratively reweighted single-step genomic BLUP procedures (ssGWAS1 and ssGWAS2, a single-marker model (CGWAS, and BayesB. The ssGWAS methods utilize genomic breeding values (GEBVs based on combined pedigree, genomic and phenotypic information, while CGWAS and BayesB only utilize phenotypes from genotyped animals or pseudo-phenotypes. In this study, ssGWAS was performed by converting GEBVs to SNP marker effects. Unequal variances for markers were incorporated for calculating weights into a new genomic relationship matrix. SNP weights were refined iteratively. The data was body weight at 6 weeks on 274,776 broiler chickens, of which 4553 were genotyped using a 60k SNP chip. Comparison of genomic regions was based on genetic variances explained by local SNP regions (20 SNPs. After 3 iterations, the noise was greatly reduced of ssGWAS1 and results are similar to that of CGWAS, with 4 out of the top 10 regions in common. In contrast, for BayesB, the plot was dominated by a single region explaining 23.1% of the genetic variance. This same region was found by ssGWAS1 with the same rank, but the amount of genetic variation attributed to the region was only 3%. These finding emphasize the need for caution when comparing and interpreting results from various methods, and highlight that detected associations, and strength of association, strongly depends on methodologies and details of implementations. BayesB appears to overly shrink regions to zero, while overestimating the amount of genetic variation attributed to the remaining SNP effects. The real world is most likely a compromise between methods and remains to

Pitting corrosion studies on nitrogen implanted 316L SS for biomedical applications

International Nuclear Information System (INIS)

Subbaiyan, M.; Veerabadran, K.M.; Thampi, N.S.; Kanwar Krishnan; Kamachi Mudali, U.; Dayal, R.K.

1997-01-01

Traditionally, human bone fracture and defects have been corrected using metal and alloy fixing devices. Austenitic stainless steels (such as 316L alloy studied here) are favoured because of low cost, compared to titanium alloys, ease of fabrication and fair corrosion resistance. Localized attack on 316l stainless steel, however, results in iron, chromium and nickel ions leaching into surrounding body fluids. This study reports on the successful use of nitrogen ion implantation into 316lSS to evaluate the optimum dose needed to minimise this localised attack, in a physiological saline solution. (UK)

Röntgenhoitajaopiskelijan työturvallisuus ammattitaitoa edistävässä harjoittelussa

OpenAIRE

Vierula, Hanna-Leena

2011-01-01

Opinnäytetyönä tuotettiin taskuinfo työturvallisuudesta Metropolia Ammattikorkeakoulun radiografian ja sädehoidon koulutusohjelman opiskelijoille ammattitaitoa edistäviin harjoitteluihin. Opinnäytetyön tavoite on edistää röntgenhoitajaopiskelijoiden tietoutta työturvallisuudesta sekä auttaa heitä työturvallisuuteen liittyvissä ongelmatilanteissa ammattitaitoa edistävissä harjoitteluissa. Opinnäytetyössä tuotettiin materiaalia opiskelijoiden työturvallisuuteen liittyen. Pohjana käytettiin...

Diagnostic value of 99mTc-pertechnetate salivary gland scintigraphy (SGS) in Sjoegren's syndrome (SS). Comparative study with symptomatic non Sjoegren patients and healthy controls

International Nuclear Information System (INIS)

Lobo, G.; Ladron de Guevara, D.; Zerboni, A.; Aguilera, S.

2002-01-01

The aim of this study was to describe SGS findings in patients with SS, and to compare them with non Sjoegren symptomatic and healthy control individual, estimating performance of SGS in SS diagnosis. Materials and Method: Fifty three control individual (average age: 53.7 yr, range: 27-83 yr) and 169 patients with subjective xerostomia underwent 99mTc-pertechnetate scintigraphy. The symptomatic group consisted of: 112 patients with Sjoegren's syndrome (average age: 53.7 yr, range:16-81 yr) according to modifying European Classification Criteria, 42 patients with fibromyalgia (FM)(average age:48.2 yr, range:19-76 yr) who presented non-specific chronic sialadenitis or normal labial biopsy, and 15 patients with keratoconjunctivitis sicca (KS) (average age: 40.9 yr, range:23-57 yr). SGS was performed following i.v. injection of 10 mCi 99mTc-pertechnetate, in dynamic acquisition of 60 15-sec frames, and giving lemon juice orally at 20 min. Irregular regions of interest (ROI) over salivary glands and brain for background assessment were drawn, building time-activity curves. SGS was classified according to visual intensity of gland tracer uptake and excretion before and after lemon and curve evaluation, in: normal (intensity of gland uptake fourfold background activity, ascending curve with fast and profound fall after lemon) , mild alteration (light decrease in gland uptake or excretion, with a normal curve shape), moderate alteration (evident uptake and excretion decrease with a median Mita curve) and severe alteration (very low or absent uptake, flat or slope curve). Scintigraphic findings were compared with diagnosis, calculating positive (PPV) and negative predictive value (NPV) for SS. Results: The results of SGS according to diagnosis are presented. SS group had higher incidence of severe alterations (p<0.001) than each of other clinics groups and lower proportion of mild alterations (p:0.008) and normal scans (p:0.005) than both control and KS patients. The KS

Burnout syndrome among dental students: a short version of the "Burnout Clinical Subtype Questionnaire" adapted for students (BCSQ-12-SS

Directory of Open Access Journals (Sweden)

Montero-Marin Jesus

2011-12-01

Full Text Available Abstract Background Burnout has been traditionally defined in relation to the dimensions of "exhaustion", "cynicism", and "inefficiency". More recently, the Burnout Clinical Subtype Questionnaire (BCSQ-12 further established three different subtypes of burnout: the "frenetic" subtype (related to "overload", the "under-challenged" subtype (related to "lack of development", and the "worn-out" subtype (related to "neglect". However, to date, these definitions have not been applied to students. The aims of this research were (1 to adapt a Spanish version of the BCSQ-12 for use with students, (2 to test its factorial validity, internal consistency, convergent and discriminant validity, and (3 to assess potential socio-demographic and occupational risk factors associated with the development of the subtypes. Method We used a cross-sectional design on a sample of dental students (n = 314 from Santiago and Huesca universities (Spain. Participants completed the Burnout Clinical Subtype Questionnaire Student Survey (BCSQ-12-SS, the Maslach Burnout Inventory Student Survey (MBI-SS, and a series of socio-demographic and occupational questions formulated for the specific purpose of this study. Data were subjected to exploratory factor analysis (EFA using the principal component method with varimax orthogonal rotation. To assess the relations with the criterion, we calculated the Pearson correlation coefficient (r, multiple correlation coefficient (Ry.123, and the coefficient of determination (R2y.123. To assess the association between the subtypes and the socio-demographic variables, we examined the adjusted odds ratio (OR obtained from multivariate logistic regression models. Results Factorial analyses supported the theoretical proposition of the BCSQ-12-SS, with α-values exceeding 0.80 for all dimensions. The "overload-exhaustion" relation was r = 0.59 (p y.123 = 0.62, 30.25% in "cynicism" (Ry.123 = 0.55, and 26.01% in "inefficiency" (Ry.123 = 0

Isolation of a new ssDNA aptamer against staphylococcal enterotoxin B based on CNBr-activated sepharose-4B affinity chromatography.

Science.gov (United States)

Hedayati Ch, Mojtaba; Amani, Jafar; Sedighian, Hamid; Amin, Mohsen; Salimian, Jafar; Halabian, Raheleh; Imani Fooladi, Abbas Ali

2016-09-01

Staphylococcus aureus are potent human pathogens possessing arsenal of virulence factors. Staphylococcal food poisoning (SFP) and respiratory infections mediated by staphylococcal enterotoxin B (SEB) are common clinical manifestations. Many diagnostic techniques are based on serological detection and quantification of SEB in different food and clinical samples. Aptamers are known as new therapeutic and detection tools which are available in different ssDNA, dsDNA and protein structures. In this study, we used a new set of ssDNA aptamers against SEB. The methods used included preparation of a dsDNA library using standard SEB protein as the target analyte, affinity chromatography matrix in microfuge tubes, SELEX procedures to isolate specific ssDNA-aptamer as an affinity ligand, aptamer purification using ethanol precipitation method, affinity binding assay using ELISA, aptamer cloning and specificity test. Among 12 readable sequences, three of them were selected as the most appropriate aptamer because of their affinity and specificity to SEB. This study presents a new set of ssDNA aptamer with favorable selectivity to SEB through 12 rounds of SELEX. Selected aptamers were used to detect SEB in infected serum samples. Results showed that SEB c1 aptamer (2 µg SEB/100 nM aptamer) had favorable specificity to SEB (kd  = 2.3 × 10(-11) ). In conclusion, aptamers can be considered as useful tools for detecting and evaluating SEB. The results showed that affinity chromatography was an affordable assay with acceptable accuracy to isolate sensitive and selective novel aptamers. Copyright © 2016 John Wiley & Sons, Ltd. Copyright © 2016 John Wiley & Sons, Ltd.

Validation of the flow-through chamber (FTC and steady-state (SS methods for clearance rate measurements in bivalves

Directory of Open Access Journals (Sweden)

Poul S. Larsen

2011-09-01

To obtain precise and reliable laboratory clearance rate (filtration rate measurements with the ‘flow-through chamber method’ (FTC the design must ensure that only inflow water reaches the bivalve's inhalant aperture and that exit flow is fully mixed. As earlier recommended these prerequisites can be checked by a plot of clearance rate (CR versus increasing through-flow (Fl to reach a plateau, which is the true CR, but we also recommend to plot percent particles cleared versus reciprocal through-flow where the plateau becomes the straight line CR/Fl, and we emphasize that the percent of particles cleared is in itself neither a criterion for valid CR measurement, nor an indicator of appropriate ‘chamber geometry’ as hitherto adapted in many studies. For the ‘steady-state method’ (SS, the design must ensure that inflow water becomes fully mixed with the bivalve's excurrent flow to establish a uniform chamber concentration prevailing at its incurrent flow and at the chamber outlet. These prerequisites can be checked by a plot of CR versus increasing Fl, which should give the true CR at all through-flows. Theoretically, the experimental uncertainty of CR for a given accuracy of concentration measurements depends on the percent reduction in particle concentration (100×P from inlet to outlet of the ideal ‘chamber geomety’. For FTC, it decreases with increasing values of P while for SS it first decreases but then increases again, suggesting the use of an intermediate value of P. In practice, the optimal value of P may depend on the given ‘chamber geometry’. The fundamental differences between the FTC and the SS methods and practical guidelines for their use are pointed out, and new data on CR for the blue mussel, Mytilus edulis, illustrate a design and use of the SS method which may be employed in e.g. long-term growth experiments at constant algal concentrations.

Optimisation of a polygon mirror-based spectral filter for swept source optical coherence tomography (SS-OCT)

Science.gov (United States)

Everson, Michael; Duma, Virgil-Florin; Dobre, George

2018-03-01

Medical imaging using Optical Coherence Tomography (OCT) provides clinicians with 3D, high resolution reconstructions of microscopic structures, in depth. It has been initially developed for ophthalmology, in order to scan the retinas of patients to diagnose illness. The quality of the images depends upon their axial and lateral resolutions and the properties of the light being used. Research using a polygon mirror (PM) as a spectral filter in Swept Source OCT (SS-OCT) has resulted in a variety of different experimental arrangements. Although the application of PM-based SS-OCT sources has been successfully demonstrated, the combination of their components' fundamental properties and the overall impact they have on imaging performance is rarely reported. A more detailed examination of these properties would lead to a full description of their operation and to the best methods to employ if system performance is to be maximised. This work presents our current findings of on-going research into the optimisation of PM-based SS-OCT systems. A swept source spectral filter, consisting of a collimator, a transmission grating, a two-lens telescope and an off-axis PM with an end reflector mirror has been evaluated experimentally and compared with theoretical predictions. The system's performance has been compared for two different fibre collimators. Although the beam width on the grating is different for each of the two collimators, the spot size at the PM facet is made the same by selecting appropriate focal lengths. An improvement in the signal roll-off at the interferometer output of 1.0 dB/mm was obtained when using a 3.4 mm collimator compared to a 1.5 mm collimator.

Rituximab Effectiveness and Safety for Treating Primary Sjögren's Syndrome (pSS: Systematic Review and Meta-Analysis.

Directory of Open Access Journals (Sweden)

Francine Bertolais do Valle Souza

Full Text Available Primary Sjögren's Syndrome (pSS is a systemic autoimmune disease that involves the exocrine glands and internal organs. pSS leads to destruction and loss of secretory function due to intense lymphoplasmacytic infiltration. Therapeutic options include mainly symptomatic and supportive measures, and traditional immunosuppressant drugs have shown no effectiveness in randomized trials. Rituximab (RTX is a chimeric antibody anti-CD20 that leads to B cell depletion by diverse mechanisms. There is evidence that this drug may be effective for treating pSS. The objective of this systematic review was to evaluate Rituximab effectiveness and safety for treating pSS.We conducted a systematic review of RCTs published until December 2015, with no language restriction. We registered a protocol on Plataforma Brasil (40654814.6.0000.5505 and developed search strategies for the following scientific databases: MEDLINE, EMBASE, CENTRAL and LILACS. We included adults with established pSS diagnosis and considered the use of Rituximab as intervention and the use of other drugs or placebo as control. Four studies met our eligibility criteria: three with low risk of bias and one with uncertain risk of bias. The total number of participants was 276 (145 RTX, 131 placebo. We assessed the risk of bias of each included study and evaluated the following as primary outcomes: lacrimal gland function, salivary gland function, fatigue improvement and adverse events. We found no significant differences between the groups in the Schirmer test at week 24 meta-analysis (MD 3.59, 95% CI -2.89 to 10.07. Only one study evaluated the lissamine green test and reported a statistically significant difference between the groups at week 24 (MD -2.00, 95% CI -3.52 to -0.48. There was a significant difference between the groups regarding salivary flow rate (MD 0.09, 95% CI 0.02 to 0.16 and improvement in fatigue VAS at weeks 6 (RR 3.98, 95% CI 1.61 to 9.82 and week 16 (RR 3.08, 95% CI 1.21 to

Lisätty todellisuus vähittäiskaupan markkinointiviestinnässä

OpenAIRE

Moisa, Teuvo

2017-01-01

Lisätty todellisuus eli AR (englanniksi augmented reality) lisää todellisuuteen virtuaalisen elementin. Vähittäiskaupan markkinointiviestinnässä lisättyä todellisuutta on käytetty useiden vuosien ajan vahvistamaan brändikokemusta. Lisätty todellisuus mahdollistaa mukaansatempaavan markkinoinnin ja lisää asiakkaiden uskollisuutta. Lisättyä todellisuutta voi hyödyntää neljän paradigman kautta, jotka ovat aktiiviset painotuotteet ja pakkaukset, virtuaalipeilit, virtuaali-ikkunat ja paikannusta k...

T3SS-dependent differential modulations of the jasmonic acid pathway in susceptible and resistant genotypes of Malus spp. challenged with Erwinia amylovora.

Science.gov (United States)

Dugé De Bernonville, Thomas; Gaucher, Matthieu; Flors, Victor; Gaillard, Sylvain; Paulin, Jean-Pierre; Dat, James F; Brisset, Marie-Noëlle

2012-06-01

Fire blight is a bacterial disease of Maloideae caused by Erwinia amylovora (Ea). This necrogenic enterobacterium uses a type III secretion system (T3SS) to inject type III effectors into the plant cells to cause disease on its susceptible hosts, including economically important crops like apple and pear. The expressions of marker genes of the salicylic acid (SA) and jasmonic acid (JA) defense regulation pathways were monitored by RT-qPCR in leaves of two apple genotypes, one susceptible and one resistant, challenged with a wild type strain, a T3SS-deficient strain or water. The transcriptional data taken together with hormone level measurements indicated that the SA pathway was similarly induced in both apple genotypes during infection by Ea. On the contrary, the data clearly showed a strong T3SS-dependent down-regulation of the JA pathway in leaves of the susceptible genotype but not in those of the resistant one. Accordingly, methyl-jasmonate treated susceptible plants displayed an increased resistance to Ea. Bacterial mutant analysis indicated that JA manipulation by Ea mainly relies on the type III effector DspA/E. Taken together, our data suggest that the T3SS-dependent down-regulation of the JA pathway is a critical step in the infection process of Malus spp. by Ea. Copyright © 2012 Elsevier Ireland Ltd. All rights reserved.

Voxel-based morphometric brain comparison between healthy subjects and major depressive disorder patients in Japanese with the s/s genotype of 5-HTTLPR.

Science.gov (United States)

Igata, Natsuki; Kakeda, Shingo; Watanabe, Keita; Ide, Satoru; Kishi, Taro; Abe, Osamu; Igata, Ryouhei; Katsuki, Asuka; Iwata, Nakao; Yoshimura, Reiji; Korogi, Yukunori

2017-06-21

Individuals with s/s genotype of serotonin transporter gene-linked promotor region (5-HTTLPR), which appear with a high frequency in Japanese, exhibit more diagnosable depression in relation to stressful life events than those with the s/l or l/l genotype. We prospectively investigated the brain volume changes in first-episode and medication naïve major depression disorder patients (MDD) with the s/s genotype in Japanese. We assessed the differences between 27 MDD with the s/s genotype and 44 healthy subjects (HS) with the same genotype using a whole-brain voxel-by-voxel statistical analysis of MRI. Gray matter volume in a brain region with significant clusters obtained via voxel-based morphometry analysis were measured and, as an exploratory analysis, evaluated for relationships to the subcategory scores (core, sleep, activity, psychic, somatic anxiety, delusion) of the Hamilton Depression Rating Scale (HAM-D) and the Social Readjustment Rating Scale (SRRS). The brain volume in the left insula lobe was significantly smaller in the MDD than in the HS. The left insula lobe volume correlated negatively with the "psychic" score of HAM-D and the SRRS. In a Japanese population with the s/s genotype, we found an atrophy of the insula in the MDD, which might be associated with "psychic" symptom and stress events.

Screening and Identifying a Novel ssDNA Aptamer against Alpha-fetoprotein Using CE-SELEX

Science.gov (United States)

Dong, Lili; Tan, Qiwen; Ye, Wei; Liu, Dongli; Chen, Haifeng; Hu, Hongwei; Wen, Duo; Liu, Yang; Cao, Ya; Kang, Jingwu; Fan, Jia; Guo, Wei; Wu, Weizhong

2015-01-01

Alpha-fetoprotein (AFP) is a liver cancer associated protein and has long been utilized as a serum tumor biomarker of disease progression. AFP is usually detected in HCC patients by an antibody based system. Recently, however, aptamers generated from systematic evolution of ligands by exponential enrichment (SELEX) were reported to have an alternative potential in targeted imaging, diagnosis and therapy. In this study, AFP-bound ssDNA aptamers were screened and identified using capillary electrophoresis (CE) SELEX technology. After cloning, sequencing and motif analysis, we successfully confirmed an aptamer, named AP273, specifically targeting AFP. The aptamer could be used as a probe in AFP immunofluorescence imaging in HepG2, one AFP positive cancer cell line, but not in A549, an AFP negative cancer cell line. More interesting, the aptamer efficiently inhibited the migration and invasion of HCC cells after in vivo transfection. Motif analysis revealed that AP273 had several stable secondary motifs in its structure. Our results indicate that CE-SELEX technology is an efficient method to screen specific protein-bound ssDNA, and AP273 could be used as an agent in AFP-based staining, diagnosis and therapy, although more works are still needed. PMID:26497223

Effect of Microstructure on the Wear Behavior of Heat Treated SS-304 Stainless Steel

Directory of Open Access Journals (Sweden)

S. Kumar

2016-12-01

Full Text Available Sliding wear characteristics of some heat treated SS-304 stainless steel against EN-8 steel in dry condition have been studied in the present experimental work. Samples of SS-304 stainless steel have been heated in a muffle furnace in desired temperature and allowed to dwell for two hours. The heated specimen are then cooled in different media namely inside the furnace, open air, cutting grade oil (grade 44 and water at room temperature to obtain different grades of heat treatment. Microstructures and corresponding micro hardness of the samples have been measured along with Feritscopic studies. Wear characteristics have been studied in a multi tribo-tester (Ducom in dry sliding condition against EN-8 steel roller. Speed, load on job and duration of test run have been considered as the experimental parameters. The wear of the samples have been obtained directly from ‘Winducom 2006’ software. Mass loss of the samples before and after operation has also been considered as the measure of wear in the present study. All the samples have been slid against EN-8 steel roller with fixed experimental parameters. The data have been plotted, compared and analyzed. Effect of microstructures as well as micro hardness on the wear behavior has been studied and concluded accordingly.

T3SS effector VopL inhibits the host ROS response, promoting the intracellular survival of Vibrio parahaemolyticus.

Directory of Open Access Journals (Sweden)

Marcela de Souza Santos

2017-06-01

Full Text Available The production of antimicrobial reactive oxygen species by the nicotinamide dinucleotide phosphate (NADPH oxidase complex is an important mechanism for control of invading pathogens. Herein, we show that the gastrointestinal pathogen Vibrio parahaemolyticus counteracts reactive oxygen species (ROS production using the Type III Secretion System 2 (T3SS2 effector VopL. In the absence of VopL, intracellular V. parahaemolyticus undergoes ROS-dependent filamentation, with concurrent limited growth. During infection, VopL assembles actin into non-functional filaments resulting in a dysfunctional actin cytoskeleton that can no longer mediate the assembly of the NADPH oxidase at the cell membrane, thereby limiting ROS production. This is the first example of how a T3SS2 effector contributes to the intracellular survival of V. parahaemolyticus, supporting the establishment of a protective intracellular replicative niche.

Localized Corrosion Behavior of Type 304SS with a Silica Layer Under Atmospheric Corrosion Environments

International Nuclear Information System (INIS)

E. Tada; G.S. Frankel

2006-01-01

The U.S. Department of Energy (DOE) has proposed a potential repository for spent nuclear fuel and high-level radioactive waste at the Yucca Mountain site in Nevada. [I] The temperature could be high on the waste packages, and it is possible that dripping water or humidity could interact with rock dust particulate to form a thin electrolyte layer with concentrated ionic species. Under these conditions, it is possible that highly corrosion-resistant alloys (CRAs) used as packages to dispose the nuclear waste could suffer localized corrosion. Therefore, to better understand long-term corrosion performance of CRAs in the repository, it is important to investigate localized corrosion under a simulated repository environment. We measured open circuit potential (OCP) and galvanic current (i g ) for silica-coated Type 304SS during drying of salt solutions under controlled RH environments to clarify the effect of silica layer as a dust layer simulant on localized corrosion under atmospheric environments. Type 304SS was used as a relatively susceptible model CRA instead of the much more corrosion resistant alloys, such as Alloy 22, that are being considered as, waste package materials

Constitutive modeling for uniaxial time-dependent ratcheting of SS304 stainless steel

International Nuclear Information System (INIS)

Kan Qianhua; Kang Guozheng; Zhang Juan

2007-01-01

Based on the experimental results of uniaxial time-dependent ratcheting behavior of SS304 stainless steel at room temperature and 973K, a new time-dependent constitutive model was proposed. The model describes the time-dependent ratcheting by adding a static/thermal recovery into the Abdel-Karim-Ohno non-linear kinematic hardening rule. The capability of the model to describe the time-dependent ratcheting was discussed by comparing the simulations with the corresponding experimental results. It is shown that the revised unified viscoplastic model can simulate the time-dependent ratcheting reasonably both at room and high temperatures. (authors)

Identification of ssDNA aptamers specific to clinical isolates of Streptococcus mutans strains with different cariogenicity.

Science.gov (United States)

Cui, Wei; Liu, Jiaojiao; Su, Donghua; Hu, Danyang; Hou, Shuai; Hu, Tongnan; Yang, Jiyong; Luo, Yanping; Xi, Qing; Chu, Bingfeng; Wang, Chenglong

2016-06-01

Streptococcus mutans, a Gram-positive facultative anaerobic bacterium, is considered to be a major etiological factor for dental caries. In this study, plaques from dental enamel surfaces of caries-active and caries-free individuals were obtained and cultivated for S. mutans isolation. Morphology examination, biochemical characterization, and polymerase chain reaction were performed to identify S. mutans The cariogenicity of S. mutans strains isolated from clinical specimens was evaluated by testing the acidogenicity, aciduricity, extracellular polysaccharide production, and adhesion ability of the bacteria. Finally, subtractive SELEX (systematic evolution of ligands by exponential enrichment) technology targeting whole intact cells was used to screen for ssDNA aptamers specific to the strains with high cariogenicity. After nine rounds of subtractive SELEX, sufficient pool enrichment was achieved as shown by radioactive isotope analysis. The enriched pool was cloned and sequenced randomly, followed by MEME online and RNA structure software analysis of the sequences. Results from the flow cytometry indicated that aptamers H1, H16, H4, L1, L10, and H19 could discriminate highly cariogenic S. mutans strains from poorly cariogenic strains. Among these, Aptamer H19 had the strongest binding capacity with cariogenic S. mutans strains with a dissociation constant of 69.45 ± 38.53 nM. In conclusion, ssDNA aptamers specific to highly cariogenic clinical S. mutans strains were successfully obtained. These ssDNA aptamers might be used for the early diagnosis and treatment of dental caries. © The Author 2016. Published by Oxford University Press on behalf of the Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.

[Mini-KiSS--a multimodal group therapy intervention for parents of young children with sleep disorders: a pilot study].

Science.gov (United States)

Schlarb, Angelika Anita; Brandhorst, Isabel; Hautzinger, Martin

2011-05-01

Sleep disorders in early childhood tend to be chronic and almost always a burden for the parents. This study developed and evaluated a multimodal parent training program for children 0.5 to 4 years of age suffering from sleep disorders (Mini-KiSS). We hypothesized that there would be specific improvements following the structured group training (reduction of sleep problems, improvement of parental well-being). The pilot study consisted of a pre-post test design without control group. Participants were n = 17 parents of children 0.5 to 4 years of age with sleep disorders determined according to the ICSD-II. Each of the six sessions was evaluated, and changes were assessed by sleep diary and CBCL. Behavioral and emotional problems of the child were assessed by CBCL, parental well-being, and SCL-90-R. The results showed high acceptance of Mini-KiSS and satisfactory feasibility. Children showed significant improvements of the sleep disturbances such as nightly awakenings as well as sleeping in parents' bed. Furthermore, improvements were found for children's emotional and behavioral problems and for parental well-being, in particular for the depression scale of the mother. This pilot study shows a high acceptance and good feasibility of the multimodal short-time parent-training program Mini-KiSS. Sleep problems were significantly reduced.

Short-time-scale Doppler and intensity variations in the spectrum of SS 433

International Nuclear Information System (INIS)

Wagner, R.M.; Newsom, G.H.; Foltz, C.B.; Byard, P.L.

1981-01-01

Spectroscopic observations of SS 433 were obtained on ten consecutive nights in 1981 May. Measurements of the equivalent widths of the spectral lines and Doppler shifts of the moving lines are presented. Not only does the 6-day period in the wavelengths of the moving lines appear quite clearly, but the data strongly suggest that this period is decreasing at a rate of (3.0 +- 1.1) x 10 -5 days/day. In addition, large equivalent width variations of the stationary lines are observed

Synthesis and antioxidant evaluation of (S,S)- and (R,R)-secoisolariciresinol diglucosides (SDGs)

OpenAIRE

Mishra, Om P.; Simmons, Nicholas; Tyagi, Sonia; Pietrofesa, Ralph; Shuvaev, Vladimir V.; Valiulin, Roman A.; Heretsch, Philipp; Nicolaou, K. C.; Christofidou-Solomidou, Melpo

2013-01-01

Secoisolariciresinol diglucosides (SDGs) (S,S)-SDG-1 (major isomer in flaxseed) and (R,R)-SDG-2 (minor isomer in flaxseed) were synthesized from vanillin via secoisolariciresinol (6) and glucosyl donor 7 through a concise route that involved chromatographic separation of diastereomeric diglucoside derivatives (S,S)-8 and (R,R)-9. Synthetic (S,S)-SDG-1 and (R,R)-SDG-2 exhibited potent antioxidant properties (EC50 = 292.17 ± 27.71 μM and 331.94 ± 21.21 μM, respectively) which compared well with...

Turvajalkineiden vaikutus jalkaterveyteen Suur-Savon Sähkötyö Oy:ssä

OpenAIRE

Pulkkinen, Riikka; Tossavainen, Inka; Ryynänen, Elina

2014-01-01

Opinnäytetyön tarkoituksena oli selvittää, millaisia vaikutuksia turvajalkineilla voi olla jalkaterveyteen, minkälaisia jalkaongelmia turvajalkineiden käyttö voi aiheuttaa, voivatko työntekijät itse vaikuttaa turvajalkineiden hankintaan ja millainen on turvajalkineiden hankintaprosessi Suur-Savon Sähkötyö Oy:ssä. Opinnäytetyömme toimeksiantaja oli Suur-Savon Sähkötyö Oy. Tutkimusmenetelmänä käytettiin pääasiassa kvantitatiivista tutkimusta ja aineisto kerättiin puolistrukturoidulla kysely...

Type-IVC Secretion System: A Novel Subclass of Type IV Secretion System (T4SS) Common Existing in Gram-Positive Genus Streptococcus

Science.gov (United States)

Chen, Chen; Gao, George F.

2012-01-01

A growing number of pathogens are being found to possess specialized secretion systems which they use in various ways to subvert host defenses. Type IV secretion system (T4SS) is one of versatile secretion systems essential for the virulence and even survival of some bacteria species, and they enable the secretion of protein and DNA substrates across the cell envelope. T4SS was once believed to be present only in Gram-negative bacteria. In this study, we present evidence of a new subclass of T4SS, Type-IVC secretion system and indicate its common existence in the Gram-positive bacterial genus Streptococcus. We further identified that VirB1, VirB4, VirB6 and VirD4 are the minimal key components of this system. Using genome comparisons and evolutionary relationship analysis, we proposed that Type-IVC secretion system is movable via transposon factors and mediates the conjugative transfer of DNA, enhances bacterial pathogenicity, and could cause large-scale outbreaks of infections in humans. PMID:23056296

Experimental study of microstructure, mechanical and tribological properties of cBN particulates SS316 alloy based MMCs fabricated by DMLS technique

Energy Technology Data Exchange (ETDEWEB)

Hussain, M.; Mandal, V.; Singh, P. K.; Kumar, P.; Kumar, V.; Das, A. K. [Indian Institute of Technology (ISM), Dhanbad (India)

2017-06-15

Direct metal laser sintering process (DMLS) was chosen to develop cBN particulates reinforced SS316 based Metal matrix composite (MMC) with 5 %, 7.5 % and 10 % cBN in the nitrogen gas atmosphere using continuous wave fibre laser of 400 W output capacity. Effects of process parameters such as laser power, beam scanning speed and the mixing ratio of powder on different physical properties of the developed MMC were investigated. It was found that the physical and mechanical properties such as friction and wear behavior, micro hardness and density come up with improved results. FESEM images indicate the microstructure of the composite and evidently confirms the presence of cubic boron nitride in the SS316 matrix where chromium nitride acted as a binder in the presence of nitrogen atmosphere. The Vickers hardness values of the developed MMCs with laser power 60 W and 65 W were found in the range of 276-478 HV{sub 0}.2 and 297-460 HV{sub 0}.2, respectively. It was found that Vickers hardness is directly proportional to the % of cBN in the powder mixture and the laser beam power. The wear resistance of the sintered MMCs increased with increasing cBN content in powder mixture and re- sults show that wear of MMCs are much lower than that of SS316. X-Ray diffraction (XRD) analysis of the fabricated MMC confirms the presence of different phases such as cBN, CrN, CrB{sub 2}, Cr{sub 2}N and Fe{sub 3}N as a consequence of a series of chemical reaction between cBN and different elements of SS316 in nitrogen atmosphere.

Experimental study of microstructure, mechanical and tribological properties of cBN particulates SS316 alloy based MMCs fabricated by DMLS technique

International Nuclear Information System (INIS)

Hussain, M.; Mandal, V.; Singh, P. K.; Kumar, P.; Kumar, V.; Das, A. K.

2017-01-01

Direct metal laser sintering process (DMLS) was chosen to develop cBN particulates reinforced SS316 based Metal matrix composite (MMC) with 5 %, 7.5 % and 10 % cBN in the nitrogen gas atmosphere using continuous wave fibre laser of 400 W output capacity. Effects of process parameters such as laser power, beam scanning speed and the mixing ratio of powder on different physical properties of the developed MMC were investigated. It was found that the physical and mechanical properties such as friction and wear behavior, micro hardness and density come up with improved results. FESEM images indicate the microstructure of the composite and evidently confirms the presence of cubic boron nitride in the SS316 matrix where chromium nitride acted as a binder in the presence of nitrogen atmosphere. The Vickers hardness values of the developed MMCs with laser power 60 W and 65 W were found in the range of 276-478 HV_0.2 and 297-460 HV_0.2, respectively. It was found that Vickers hardness is directly proportional to the % of cBN in the powder mixture and the laser beam power. The wear resistance of the sintered MMCs increased with increasing cBN content in powder mixture and re- sults show that wear of MMCs are much lower than that of SS316. X-Ray diffraction (XRD) analysis of the fabricated MMC confirms the presence of different phases such as cBN, CrN, CrB_2, Cr_2N and Fe_3N as a consequence of a series of chemical reaction between cBN and different elements of SS316 in nitrogen atmosphere.

HHF test with 80x80x1 Be/Cu/SS Mock-ups for verifying the joining technology of the ITER blanket First Wall

Energy Technology Data Exchange (ETDEWEB)

Lee, Dong Won; Bae, Young Dug; Kim, Suk Kwon; Hong, Bong Guen; Jeong, Yong Hwan; Park, Jeong Yong; Choi, Byung Kwon; Jung, Hyun Kyu

2008-11-15

Through the fabrication of the Cu/SS and Be/Cu joint specimens, fabrication procedure such as material preparation, canning, degassing, HIP (Hot Isostatic Pressing), PHHT (Post HIP heat treatment) was established. The HIP conditions (1050 .deg. C, 100 MPa 2 hr for Cu/SS, 580 .deg. C 100 MPa 2 hr for Be/Cu) were developed through the investigation on joint specimen fabricated with the various HIP conditions; the destructive tests of joint include the microstructure observation of the interface with the examination of the elemental distribution, tension test, bend test, Charpy impact test and fracture toughness test. However, since the joint should be tested under the High Heat Flux (HHF) conditions like the ITER operation for verifying its joint integrity, several HHF tests were performed like the previous HHF test with the Cu/SS, Be/Cu, Be/Cu/SS Mock-ups. In the present study, the HHF test with Be/Cu/SS Mock-ups, which have 80 mm x 80 mm single Be tile and each material depths were kept to be the same as the ITER blanket FW. The Mock-ups fabricated with three kinds of interlayers such as Cr/Ti/Cu, Ti/Cr/Cu, Ti/Cu, which were different from the developed interlayer (Cr/Cu), total 6 Mock-ups were fabricated. Preliminary analysis were performed to decide the test conditions; they were tested with up to 2.5 MW/m2 of heat fluxes and 20 cycles for each Mock-up in a given heat flux. They were tested with JUDITH-1 at FZJ in Germany. During tests, all Mock-ups showed delamination or full detachment of Be tile and it can be concluded that the joints with these interlayers have a bad joining but it can be used as a good data for developing the Be/Cu joint with HIP.

3D-FE Modeling of 316 SS under Strain-Controlled Fatigue Loading and CFD Simulation of PWR Surge Line

Energy Technology Data Exchange (ETDEWEB)

Mohanty, Subhasish [Argonne National Lab. (ANL), Argonne, IL (United States); Barua, Bipul [Argonne National Lab. (ANL), Argonne, IL (United States); Listwan, Joseph [Argonne National Lab. (ANL), Argonne, IL (United States); Majumdar, Saurin [Argonne National Lab. (ANL), Argonne, IL (United States); Natesan, Ken [Argonne National Lab. (ANL), Argonne, IL (United States)

2017-03-01

In financial year 2017, we are focusing on developing a mechanistic fatigue model of surge line pipes for pressurized water reactors (PWRs). To that end, we plan to perform the following tasks: (1) conduct stress- and strain-controlled fatigue testing of surge-line base metal such as 316 stainless steel (SS) under constant, variable, and random fatigue loading, (2) develop cyclic plasticity material models of 316 SS, (3) develop one-dimensional (1D) analytical or closed-form model to validate the material models and to understand the mechanics associated with 316 SS cyclic hardening and/or softening, (4) develop three-dimensional (3D) finite element (FE) models with implementation of evolutionary cyclic plasticity, and (5) develop computational fluid dynamics (CFD) model for thermal stratification, thermal-mechanical stress, and fatigue of example reactor components, such as a PWR surge line under plant heat-up, cool-down, and normal operation with/without grid-load-following. This semi-annual progress report presents the work completed on the above tasks for a 316 SS laboratory-scale specimen subjected to strain-controlled cyclic loading with constant, variable, and random amplitude. This is the first time that the accurate 3D-FE modeling of the specimen for its entire fatigue life, including the hardening and softening behavior, has been achieved. We anticipate that this work will pave the way for the development of a fully mechanistic-computer model that can be used for fatigue evaluation of safety-critical metallic components, which are traditionally evaluated by heavy reliance on time-consuming and costly test-based approaches. This basic research will not only help the nuclear reactor industry for fatigue evaluation of reactor components in a cost effective and less time-consuming way, but will also help other safety-related industries, such as aerospace, which is heavily dependent on test-based approaches, where a single full-scale fatigue test can cost

Voyage on the SS "School Library Leadership": Collaboration in Teaching and Learning at the University of Vermont

Science.gov (United States)

Kaplan, Judith L.; Ballard, Susan D.

2012-01-01

This article describes the SS "School Library Leadership" maiden voyage, which departed from the University of Vermont (UVM) during the 2010 fall semester. Twelve intrepid sailors followed their sense of adventure into uncharted waters with cocaptains Judy Kaplan and Susan Ballard in an online collaboration that provided a powerful…

First report of Metarhizium anisopliae IP 46 pathogenicity in adult Anopheles gambiae s.s. and An. arabiensis (Diptera; Culicidae

Directory of Open Access Journals (Sweden)

Lyimo Issa N

2009-12-01

Full Text Available Abstract The entomopathogenic fungus Metarhizium anisopliae isolate IP 46, originating from a soil sample collected in 2001 in the Cerrado of Central Brazil, was tested for its ability to reduce the survival of adult male and female Anopheles gambiae s.s. and An. arabiensis mosquitoes. A 6-h exposure to the fungus coated on test paper at a concentration of 3.3 × 106 conidia cm-2 reduced the daily survival of both mosquito species (HR = 3.14, p An. gambiae s.s relative to An. arabiensis (HR = 1.38, p 95% of mosquito cadavers in the treatment groups. The results indicate that M. anisopliae IP 46 has the potential to be a bio-control agent for African malaria vector species, and is a suitable candidate for further research and development.

Intra-instar larval cannibalism in Anopheles gambiae (s.s.) and Anopheles stephensi (Diptera: Culicidae).

Science.gov (United States)

Porretta, Daniele; Mastrantonio, Valentina; Crasta, Graziano; Bellini, Romeo; Comandatore, Francesco; Rossi, Paolo; Favia, Guido; Bandi, Claudio; Urbanelli, Sandra

2016-11-02

Cannibalism has been observed in a wide range of animal taxa and its importance in persistence and stability of populations has been documented. In anopheline malaria vectors the inter-instar cannibalism between fourth- and first-instar larvae (L4-L1) has been shown in several species, while intra-instar cannibalism remains poorly investigated. In this study we tested the occurrence of intra-instar cannibalism within larvae of second-, third- and fourth-instar (L2, L3 and L4) of Anopheles gambiae (s.s.) and An. stephensi. Experiments were set up under laboratory conditions and the effects of larval density, duration of the contact period among larvae and the presence of an older larva (i.e. a potential cannibal of bigger size) on cannibalism rate were analysed. Cannibalism was assessed by computing the number of missing larvae after 24 and 48 h from the beginning of the experiments and further documented by records with a GoPro videocamera. Intra-instar cannibalism was observed in all larval instars of both species with higher frequency in An. gambiae (s.s.) than in An. stephensi. In both species the total number of cannibalistic events increased from 0-24 to 0-48 h. The density affected the cannibalism rate, but its effect was related to the larval instar and to the presence of older larvae. Interestingly, the lower cannibalism rate between L4 larvae was observed at the highest density and the cannibalism rate between L3 larvae decreased when one L4 was added. The present study provides experimental evidence of intra-instar cannibalism in the malaria vectors An. gambiae (s.s.) and An. stephensi and highlights the possible occurrence of complex interactions between all larval instars potentially present in the breeding sites. We hypothesize that the high density and the presence of a potential cannibal of bigger size could affect the readiness to attack conspecifics, resulting into low risk larval behavior and lower cannibalism rate. The understanding of

Intra-instar larval cannibalism in Anopheles gambiae (s.s. and Anopheles stephensi (Diptera: Culicidae

Directory of Open Access Journals (Sweden)

Daniele Porretta

2016-11-01

Full Text Available Abstract Background Cannibalism has been observed in a wide range of animal taxa and its importance in persistence and stability of populations has been documented. In anopheline malaria vectors the inter-instar cannibalism between fourth- and first-instar larvae (L4-L1 has been shown in several species, while intra-instar cannibalism remains poorly investigated. In this study we tested the occurrence of intra-instar cannibalism within larvae of second-, third- and fourth-instar (L2, L3 and L4 of Anopheles gambiae (s.s. and An. stephensi. Experiments were set up under laboratory conditions and the effects of larval density, duration of the contact period among larvae and the presence of an older larva (i.e. a potential cannibal of bigger size on cannibalism rate were analysed. Cannibalism was assessed by computing the number of missing larvae after 24 and 48 h from the beginning of the experiments and further documented by records with a GoPro videocamera. Results Intra-instar cannibalism was observed in all larval instars of both species with higher frequency in An. gambiae (s.s. than in An. stephensi. In both species the total number of cannibalistic events increased from 0–24 to 0–48 h. The density affected the cannibalism rate, but its effect was related to the larval instar and to the presence of older larvae. Interestingly, the lower cannibalism rate between L4 larvae was observed at the highest density and the cannibalism rate between L3 larvae decreased when one L4 was added. Conclusions The present study provides experimental evidence of intra-instar cannibalism in the malaria vectors An. gambiae (s.s. and An. stephensi and highlights the possible occurrence of complex interactions between all larval instars potentially present in the breeding sites. We hypothesize that the high density and the presence of a potential cannibal of bigger size could affect the readiness to attack conspecifics, resulting into low risk larval behavior

Kätilön ammatillinen osaaminen naistentautien hoitotyössä : kysely valmistuville kätilöopiskelijoille : taustatietojen yhteys ammatilliseen osaamiseen

OpenAIRE

Jerrman, Anne; Kokkonen, Saija

2008-01-01

Opinnäytetyön tarkoituksena oli kartoittaa eri taustamuuttujien yhteyttä kätilöopiskelijoiden ammatilliseen osaamiseen. Opinnäytetyö on osa Näyttöön perustuva kätilötyö -hanketta. Hanke toteutetaan yhteistyössä HUS Naistensairaalan kanssa, ja sen tavoitteena on tuottaa näyttöön perustuvaa tietoa toiminnan kehittämiseksi. Tämä työ on jatkoa opinnäytetyölle Kätilön ammatillinen osaaminen naistentautien hoitotyössä - kysely valmistuville kätilöopiskelijoille. Opinnäytetyön aineisto kerä...

Evaluation of the influence of electric nets on the behaviour of oviposition site seeking Anopheles gambiae s.s

Science.gov (United States)

2014-01-01

Background Electric nets (e-nets) are used to analyse the flight behaviour of insects and have been used extensively to study the host-oriented flight of tsetse flies. Recently we adapted this tool to analyse the oviposition behaviour of gravid malaria vectors, Anopheles gambiae s.s., orienting towards aquatic habitats and traps by surrounding an artificial pond with e-nets and collecting electrocuted mosquitoes on sticky boards on the ground next to the nets. Here we study whether e-nets themselves affect the responses of gravid An. gambiae s.s.. Methods Dual-choice experiments were carried out in 80 m2 screened semi-field systems where 200 gravid An. gambiae s.s. were released each night for 12 nights per experiment. The numbers of mosquito landing on or approaching an oviposition site were studied by adding detergent to the water in an artificial pond or surrounding the pond with a square of e-nets. We also assessed whether the supporting framework of the nets or the sticky boards used to retain electrocuted mosquitoes influenced the catch. Results Two similar detergent treated ponds presented in choice tests caught an equal proportion of the mosquitoes released, whereas a pond surrounded by e-nets caught a higher proportion than an open pond (odds ratio (OR) 1.7, 95% confidence interval (CI) 1.1 - 2.7; p electric nets and the yellow boards on the approach of gravid females towards a pond suggests that the tower-like construction of the square of electric nets did not restrict the approach of females but the yellow sticky boards on the ground attract gravid females to a source of water (OR 2.7 95% CI 1.7 – 4.3; p trapping efficiency of the electric nets is increased when large yellow sticky boards are placed on the ground next to the e-nets to collect electrocuted mosquitoes, possibly because of increased visual contrast to the aquatic habitat. It is therefore important when comparing two treatments that the same trapping device is used in both. The

Sodium confluent rates of flow values, on 0,5 mixer, of a sodium italian SS-050 circuit component

International Nuclear Information System (INIS)

Walsh, L.M.

1987-01-01

Sodium lines on different temperatures, during an emergency drainage on 0,5 mixer was found. To future valuation by DIMEC of tensions that occurs on that component of SS-050, the confluent rates of flow values were calculated. (L.M.J.) [pt

eCl rate at ss, a tool for the support of processes in eCommerce, materials management and plant documentation in view of process control; eCl rate at ss - ein Werkzeug zur Unterstuetzung der Prozesse im eCommerce, der Materialwirtschaft und der Anlagendokumentation, bezogen auf das PLT-Gewerk

Energy Technology Data Exchange (ETDEWEB)

Eibl, M.; Westphal, D.; Zgorzelski, P. [Bayer AG, Leverkusen (Germany); Kaptein, U. [Degussa-Huels AG, Marl (Germany). Konzernbereich Beschaffung und Logistik; Rudolf, H.J. [ATplan Ges. fuer Planung in der Automatisierungstechnik mbH, Leverkusen (Germany)

2000-07-01

The increasing electronic exchange of data for the description of goods between different areas of an enterprise and between enterprises leads to the necessity to standardise data structures. This was the impact for the development of a classification system including attribute lists for products, goods and services. As a part of this project, a classification system and sets of attributes for process engineering and process control equipment for the purposes of procurement and technical materials management were developed. In a project of German enterprises a classification with sets of attributes was developed, which enables a simplification of the processes in the enterprise and beyond its boundaries in different applications up to eCommerce. The structure is called 'eCl rate at ss'. With the sets of attributes and the classification hierarchy of eCl rate at ss goods can be described in a standardised way. (orig.) [German] Der zunehmende elektronische Austausch von Daten zur Beschreibung von Guetern zwischen verschiedenen Bereichen eines Unternehmens und zwischen den Unternehmen fuehrt zu der Notwendigkeit, Datenstrukturen zu standardisieren. Dies war der Anstoss zur Erarbeitung einer einheitlichen Klassifikation mit Merkmalleisten fuer Produkte, Gueter und Dienstleistungen, darunter auch fuer verfahrenstechnische und prozessleittechnische Geraete, fuer die Zwecke der Beschaffung und der technischen Materialwirtschaft. In einem Projekt der deutschen Wirtschaft wurde eine Klassifikation mit Merkmalleisten entwickelt, die in verschiedenen Anwendungen bis hin zu eCommerce eine Vereinfachung der Prozesse im Unternehmen und ueber seine Grenzen hinaus ermoeglicht. Sie erhielt den Namen 'eCl rate at ss'. Mit den Merkmalleisten und der darueberliegenden Klassifikationshierarchie von eCl rate at ss koennen Gueter in einer standardisierten Art und Weise beschrieben werden. (orig.)

Preliminary experimental results of Sewage Sludge (SS) Co-digestion with Palm Oil Mill Effluent (POME) for Enhanced Biogas Production in Laboratory Scale Anaerobic Digester

International Nuclear Information System (INIS)

Sivasankari, R; Kumaran, P; Normanbhay, Saifuddin; Shamsuddin, Abd Halim

2013-01-01

An investigation on the feasibility of co-digesting Sewage Sludge with Palm Oil Mill Effluent for enhancing the biogas production and the corresponding effect of the co-digestion substrate ratio on the biogas production has been evaluated. Anaerobic co-digestion of POME with SS was performed at ratios of 100:0, 70:30, 60:40 and 0:100 to find the optimum blend required for enhanced waste digestion and biogas production. Single stage batch digestion was carried out for 12 days in a laboratory scale anaerobic digester. Co-digestion of sludge's at the 70:30 proportion resulted in optimal COD and C: N ratio which subsequently recorded the highest performance with regards to biogas production at 28.1 L's compared to the 1.98 L's of biogas produced from digestion of SS alone. From the results obtained, it is evident that co-digestion of POME and SS is an attractive option to be explored for enhancement of biogas production in anaerobic digesters.

The post-transcriptional regulator rsmA/csrA activates T3SS by stabilizing the 5' UTR of hrpG, the master regulator of hrp/hrc genes, in Xanthomonas.

Directory of Open Access Journals (Sweden)

Maxuel O Andrade

2014-02-01

Full Text Available The RsmA/CsrA family of the post-transcriptional regulators of bacteria is involved in the regulation of many cellular processes, including pathogenesis. In this study, we demonstrated that rsmA not only is required for the full virulence of the phytopathogenic bacterium Xanthomonas citri subsp. citri (XCC but also contributes to triggering the hypersensitive response (HR in non-host plants. Deletion of rsmA resulted in significantly reduced virulence in the host plant sweet orange and a delayed and weakened HR in the non-host plant Nicotiana benthamiana. Microarray, quantitative reverse-transcription PCR, western-blotting, and GUS assays indicated that RsmA regulates the expression of the type 3 secretion system (T3SS at both transcriptional and post-transcriptional levels. The regulation of T3SS by RsmA is a universal phenomenon in T3SS-containing bacteria, but the specific mechanism seems to depend on the interaction between a particular bacterium and its hosts. For Xanthomonads, the mechanism by which RsmA activates T3SS remains unknown. Here, we show that RsmA activates the expression of T3SS-encoding hrp/hrc genes by directly binding to the 5' untranslated region (UTR of hrpG, the master regulator of the hrp/hrc genes in XCC. RsmA stabilizes hrpG mRNA, leading to increased accumulation of HrpG proteins and subsequently, the activation of hrp/hrc genes. The activation of the hrp/hrc genes by RsmA via HrpG was further supported by the observation that ectopic overexpression of hrpG in an rsmA mutant restored its ability to cause disease in host plants and trigger HR in non-host plants. RsmA also stabilizes the transcripts of another T3SS-associated hrpD operon by directly binding to the 5' UTR region. Taken together, these data revealed that RsmA primarily activates T3SS by acting as a positive regulator of hrpG and that this regulation is critical to the pathogenicity of XCC.

Corrosion Testing of 304L SS 3013 Inner Container and Teardrop Samples

Energy Technology Data Exchange (ETDEWEB)

Tokash, Justin Charles [Los Alamos National Lab. (LANL), Los Alamos, NM (United States); Hill, Mary Ann [Los Alamos National Lab. (LANL), Los Alamos, NM (United States); Lillard, Scott [Univ. of Akron, OH (United States); Joyce, Stephen Anthony [Los Alamos National Lab. (LANL), Los Alamos, NM (United States); Tegtmeier, Eric Lee [Los Alamos National Lab. (LANL), Los Alamos, NM (United States); Berg, John M. [Los Alamos National Lab. (LANL), Los Alamos, NM (United States); Veirs, Douglas Kirk [Los Alamos National Lab. (LANL), Los Alamos, NM (United States); Worl, Laura Ann [Los Alamos National Lab. (LANL), Los Alamos, NM (United States)

2017-06-27

The Department of Energy (DOE) 3013 Standard specifies a minimum of two containers to be used for the storage of plutonium-bearing materials containing at least 30 wt.% plutonium and uranium. Three nested containers are typically used, the outer, inner, and convenience containers, shown in Figure 1. Both the outer and inner containers are sealed with a weld while the innermost convenience container must not be sealed. Lifetime of the containers is expected to be fifty years. The containers are fabricated of austenitic stainless steels (SS) due to their high corrosion resistance. Potential failure mechanisms of the storage containers have been examined by Kolman and Lillard et al.

Correlation among the cytokines levels in the bronco-alveolar lavage in a cohort of patient of the Central Military Hospital, with systemic sclerosis (SS), with and without alveolitis

International Nuclear Information System (INIS)

Mejia, Rossana; Velez, Patricia; Avila, Luz Mabel; Pernett, Frank; Giraldo, Luis Fernando; Amaya, Betty; Mejia, Adriana; Londono J, John; Valle, Rafael

2003-01-01

Systemic sclerosis (SS) is a generalized connective tissue disorder characterized clinically by thickening and fibrosis of the skin and by distinctive forms of involvement of internal organs, notably the heart, lungs, kidneys and gastrointestinal tract. We analyzed nineteen patients with SS 9 with SSI, 10 with SSd and two healthy controls. All patients were evaluated by comprise of skin, heart, lungs, and kidneys. Pulmonary assessments were performed with chest radiography, high-resolution computerized tomography, vital capacity, forced expiratory volume and bronchoalveolar lavage (cellular counts and measured interleukin). The interleukin's levels of IL 6, IL 1 β IL 8, and TNFα were found increased in all patients with SS, but not in healthy controls. The interleukin's level in SSd patients tended to be higher than in SSI patients, but this difference only reach statistical significance with interleukins 6. Lung involvement in SS is associated with an increase in interleukin's levels in BAL fluids, but this difference only reach statistical significance with the interleukins 6 lL 1β and TNFα

PREFACE: The 4th Symposium on the Mechanics of Slender Structures (MoSS2013)

Science.gov (United States)

Cao, Dengqing; Kaczmarczyk, Stefan

2013-07-01

This volume of Journal of Physics: Conference Series contains papers presented at the 4th Symposium on the Mechanics of Slender Structures (MoSS2013) run under the auspices of the Institute of Physics Applied Mechanics Group and hosted by Harbin Institute of Technology (China) from 7-9 January 2013. The conference has been organized in collaboration with the Technical Committee on Vibration and Sound of the American Society of Mechanical Engineers and follows a one day seminar on Ropes, Cables, Belts and Chains: Theory and Applications and the MoSS2006 symposium held at the University of Northampton (UK) in 2004 and 2006, respectively, the MoSS2008 symposium held at the University of Maryland Baltimore County (USA) in 2008 and the MoSS2010 symposium hosted by Mondragon University and held in San Sebastian (Spain) in 2010. The remit of the Symposium on the Mechanics of Slender Structures series involves a broad range of scientific areas. Applications of slender structures include terrestrial, marine and space systems. Moving elastic elements such as ropes, cables, belts and tethers are pivotal components of many engineering systems. Their lengths often vary when the system is in operation. The applications include vertical transportation installations and, more recently, space tether propulsion systems. Traction drive elevator installations employ ropes and belts of variable length as a means of suspension, and also for the compensation of tensile forces over the traction sheave. In cranes and mine hoists, cables and ropes are subject to length variation in order to carry payloads. Tethers experiencing extension and retraction are important components of offshore and marine installations, as well as being proposed for a variety of different space vehicle propulsion systems based on different applications of momentum exchange and electrodynamic interactions with planetary magnetic fields. Furthermore, cables and slender rods are used extensively in civil engineering

Interdependence of the kinetics of NTP hydrolysis and the stability of the RecA-ssDNA complex.

Science.gov (United States)

Katz, F S; Bryant, F R

2001-09-18

The ssDNA-dependent NTP hydrolysis activity of the RecA protein was examined using a series of dTn oligomers ranging in size from dT10 to dT2000 as the ssDNA effector. There were three distinct manifestations of the dTn-dependent NTP hydrolysis reaction, depending on the length of the dTn effector that was used. With longer dTn oligomers, NTP hydrolysis occurred with a turnover number of 20-25 min(-1) and the observed S0.5 value for the NTP was independent of the concentration of the dTn oligomer (DNA concentration-independent hydrolysis). With dTn oligomers of intermediate length, NTP hydrolysis still occurred with a turnover number of 20-25 min(-1), but the observed S0.5 for the NTP decreased with increasing dTn concentration until reaching a value similar to that obtained with the longer dTn oligomers (DNA concentration-dependent hydrolysis). With shorter dTn oligomers, the NTP hydrolysis activity was effectively eliminated. Although this general progression of kinetic behavior was observed for the three structurally related NTPs (dATP, ATP, and GTP), the dTn oligomer length at which DNA concentration-independent, DNA concentration-dependent, and no NTP hydrolysis was observed depended on the NTP being considered. For example, dATP (S0.5 = 35 microM) was hydrolyzed in the presence of dT20, whereas ATP (S0.5 = 70 microM) and GTP (S0.5 = 1200 microM) required at least dT50 and dT200 for hydrolysis, respectively. These results are discussed in terms of a kinetic model in which the stability of the RecA-ssDNA-NTP complex is dependent on the intrinsic S0.5 value of the NTP being hydrolyzed.

A Study on Thermal Desorption of Deuterium in D-loaded SS316LN for ITER Tritium Removal System

Energy Technology Data Exchange (ETDEWEB)

Park, Myungchul; Kim, Heemoon; Ahn, Sangbok [Korea Atomic Energy Research Institute, Daejeon (Korea, Republic of); Kim, Jaeyong; Lee, Sanghwa; LanAhn, Nguyen Thi [Hanyang University, Seoul (Korea, Republic of)

2016-10-15

Because Type B radwaste includes tritium on its inside, especially at vicinity of surface, tritium removal from the radwaste is a matter of concern in terms of the radwaste processes. Tritium behavior in materials is related with temperature. Considering a diffusion process, it is expected that tritium removal efficiency is enhanced with increasing baking temperature. However, there is a limitation about temperature due to facility capacity and economic aspect. Therefore, it is necessary to investigate the effect of temperature on the desorption behavior of Tritium in ITER materials. TDS analysis was performed in SS316LN loaded at 120, 240 and 350 °C. D2 concentration and the desorption peak temperature increased with increasing loading temperature. Using peak shift method with three ramp rates of 0.166, 0.332, and 0.5 °C/sec, trap activation energy of D in SS316LN loaded at 350 °C was 56 kJ/mol.

Expression, purification and preliminary crystallographic analysis of the T6SS effector protein Tse3 from Pseudomonas aeruginosa

International Nuclear Information System (INIS)

Lu, Defen; Shang, Guijun; Yu, Qian; Zhang, Heqiao; Zhao, Yanyu; Cang, Huaixing; Gu, Lichuan; Xu, Sujuan; Huang, Yan

2013-01-01

Tse3, one of the effectors of the type VI secretion system in Pseudomonas aeruginosa, has been crystallized and diffracted to 1.5 Å resolution. Pseudomonas aeruginosa uses the type VI secretion system (T6SS) to inject effector proteins into rival cells in niche competition. Tse3, one of the effectors of T6SS, is delivered into the periplasm of recipient cells. Tse3 functions as a muramidase that degrades the β-1,4-linkage between N-acetylmuramic acid (MurNAc) and N-acetylglucosamine (GlcNAc) in peptidoglycan, thus leading to lysis of the recipient cells and providing a competitive advantage to the donor cells. Here, the preliminary crystallographic study of Tse3 is reported. A crystal of Tse3 diffracted to 1.5 Å resolution. It belonged to space group C121, with unit-cell parameters a = 166.99, b = 70.13, c = 41.94 Å, α = 90.00, β = 90.52, γ = 90.00° and one molecule per asymmetric unit

Dynamic rockfall risk assessment along the SS113 coastal road (Northern Sicily)

Science.gov (United States)

Mastrolembo, V. Brunella; Penna, Ivanna; Voumard, Jérémie; Jaboyedoff, Michel

2016-04-01

Rockfalls are natural hazards that usually affect only small areas. However, due to the big amount of material that can be moved and the associated kinetic energy they can cost serious damages to infrastructures and people. Even fairly small rockfall fragments are a significant hazard if deposited on a highway or along a rail-road track since they are not easily detected and can cause accidents or derailments. Rockfalls can also cause the closure of streets resulting in long term indirect economic losses due to transportation delays as well as to the impact on the commercial and tourist activities. In letterature there are numerous examples of rockfall risk assessments along transportation corridors, most of them are based on the use of standard risk estimation methods. The latters are static approaches founded on a macroscopic view of road traffic, assumed as uniform in space and time, thus characterized by average values of parameters. Lately, a new dynamic approach has been developed within the 'risk analysis group' at the University of Lausanne (Voumard, 2013). It consists of a kinematic interpretation of road traffic where vehicles are parametrized as single entities with different characteristics, speed, dimensions and behaviour. We apply this new approach to estimate the dynamic risk due to rockfall occurrence on the SS113 national road running along the northern coast of Sicily. In this work we focus our attention on a ≈10 km section along which the SS113 road and a railway connect all the costal villages going through very steep cliffs and very close to the sea with evident problems of erosion and maintenance. The area is a tourist destination and many hotels and facilities are found along the road. Moreover the area was already hit in the past by numerous rockfalls resulting in the closure of the road for periods running from a few days up to a few years with big direct and indirect damages to the local socio-economic activities. In order to achieve a

New players in the same old game: a system level in silico study to predict type III secretion system and effector proteins in bacterial genomes reveals common themes in T3SS mediated pathogenesis.

Science.gov (United States)

Sadarangani, Vineet; Datta, Sunando; Arunachalam, Manonmani

2013-07-26

Type III secretion system (T3SS) plays an important role in virulence or symbiosis of many pathogenic or symbiotic bacteria [CHM 2:291-294, 2007; Physiology (Bethesda) 20:326-339, 2005]. T3SS acts like a tunnel between a bacterium and its host through which the bacterium injects 'effector' proteins into the latter [Nature 444:567-573, 2006; COSB 18:258-266, 2008]. The effectors spatially and temporally modify the host signalling pathways [FEMS Microbiol Rev 35:1100-1125, 2011; Cell Host Microbe5:571-579, 2009]. In spite its crucial role in host-pathogen interaction, the study of T3SS and the associated effectors has been limited to a few bacteria [Cell Microbiol 13:1858-1869, 2011; Nat Rev Microbiol 6:11-16, 2008; Mol Microbiol 80:1420-1438, 2011]. Before one set out to perform systematic experimental studies on an unknown set of bacteria it would be beneficial to identify the potential candidates by developing an in silico screening algorithm. A system level study would also be advantageous over traditional laboratory methods to extract an overriding theme for host-pathogen interaction, if any, from the vast resources of data generated by sequencing multiple bacterial genomes. We have developed an in silico protocol in which the most conserved set of T3SS proteins was used as the query against the entire bacterial database with increasingly stringent search parameters. It enabled us to identify several uncharacterized T3SS positive bacteria. We adopted a similar strategy to predict the presence of the already known effectors in the newly identified T3SS positive bacteria. The huge resources of biochemical data [FEMS Microbiol Rev 35:1100-1125, 2011; Cell Host Microbe 5:571-579, 2009; BMC Bioinformatics 7(11):S4, 2010] on the T3SS effectors enabled us to search for the common theme in T3SS mediated pathogenesis. We identified few cellular signalling networks in the host, which are manipulated by most of the T3SS containing pathogens. We went on to look for

Transmutation and activation of stainless steel 316 SS in a thermal fusion reactor blanket

International Nuclear Information System (INIS)

Gruber, J.; Schneider, J.

1977-10-01

Using the program MATEXP (matrix exponential method) the influence of neutron flux is calculated for stainless steel 3s16 SS which is used as a structural material in a fusion reactor blanket (CTRD-I). The transmutations, activations and γ-dose rates are determined for an operation time of 20 years. Investigating the decay behaviour after operation time, we found that the long term activity and dose rate was mainly influenced by five nuclides: Fe55, Ni63, Ni59, Co60 and Nb94. (orig.) [de

Oxide induced corrosion on the welded stainless steels SS 2352 and 2353

International Nuclear Information System (INIS)

Stroem, S.; Li Huiqin.

1991-01-01

The pitting corrosion properties have been investigated in welded and unwelded condition by polarization tests in sodium chloride solutions. The two steels were TIG welded without adding welding material and as shielding on the bottom side argon gas containing 2, 26 or 99 ppm oxygen was used. In some tests low breakthrough potentials were received, without discovering any pitting corrosion in the specimen surfaces. The unwelded SS 2352 steel had a critical (lowest) pitting temperature (CPT) of 5 degrees C in the more concentrated solution. For the same steel with weld pitting corrosion was obtained at 5 degrees C, which was the lowest temperature for the tests. Thus the CPT value was lower than 5 degrees C, but by looking at the pitting corrosion potentials the following conclusion could be drawn: Welding with higher oxygen content in the shielding gas implied lower pitting corrosion resistance. For the SS 2353 steel the CPT values were 25 and 27.5 degrees C for material without weld, in contact with the more concentrated and the more dilute solution respectively. Welded material was all through more sensitive to pitting corrosion, and the CPT values were 15-17.5, 15 and 5-10 degrees C for welded areas which had been gas shielded with argon containing 2, 26 and 99 ppm oxygen respectively. The result thus showed that welding with shielding gas containing maximum about 30 ppm oxygen does not substantially affect the pitting corrosion properties. Post treatment of the welding areas increased the pitting corrosion resistance. Acid pickling implied the highest pitting corrosion resistance with 15 degrees C as CPT value for the 2353 steel in the more concentrated solution. Steel brushing implied an obvious increase to the pitting corrosion resistance compared to untreated weld areas and the same statement could be done for sand blasted surfaces. (10 refs., 16 tabs., 11 figs.)

Multi-pass TIG welding process: simulating thermal SS304

International Nuclear Information System (INIS)

Harinadh, Vemanaboina; Akella, S.; Buddu, Ramesh Kumar; Edision, G.

2015-01-01

Welding is basic requirement in the construction of nuclear reactors, power plants and structural components development. A basic studies on various aspects of the welding is essential to ensure the stability and structural requirement conditions. The present study explored the thermo-mechanical analysis of the multipass welds of austenitic stainless steels which are widely used in fusion and fission reactor components development. A three-dimensional (3D) finite element model is developed to investigate thermally induced stress field during TIG welding process for SS304 material. The transient thermal analysis is performed to obtain the temperature history, which then is applied to the mechanical (stress) analysis. The present thermal analysis is conducted using element type DC3D8. This element type has a three dimensional thermal conduction capability and eight nodes. The 6 mm thick plated is welded with six numbers of passes. The geometry and meshed model with tetrahedral shape with volume sweep. The analysis is on TIG welding process using 3D-weld interface plug-in on ABAQUS-6.14. The results are reported in the present paper

Perehdytys etätyössä : case Annanpura Oy:n perehdytyssuunnitelma

OpenAIRE

Kohomäki, Noora

2013-01-01

Opinnäytetyön tavoitteena oli laatia toimiva perehdytyssuunnitelma yritykselle, jonka työntekijät työskentelevät pääsääntöisesti kotoaan käsin. Etätyön perehdyttämisessä korostuvat riittävän viestinnän ja vuorovaikutuksen järjestäminen. Työn keskeisenä haasteena oli dialogisen perehdytyksen ja itsenäisesti suoritettavan etätyön yhdistäminen. Työn tavoitteena oli lisäksi selvittää, onnistuuko hiljaisen tiedon ja osaamisen siirtäminen etätyössä ja sen perehdytyksessä. Teoreettisessa viitekeh...

Predicting protein aggregation during storage in lyophilized solids using solid state amide hydrogen/deuterium exchange with mass spectrometric analysis (ssHDX-MS).

Science.gov (United States)

Moorthy, Balakrishnan S; Schultz, Steven G; Kim, Sherry G; Topp, Elizabeth M

2014-06-02

Solid state amide hydrogen/deuterium exchange with mass spectrometric analysis (ssHDX-MS) was used to assess the conformation of myoglobin (Mb) in lyophilized formulations, and the results correlated with the extent of aggregation during storage. Mb was colyophilized with sucrose (1:1 or 1:8 w/w), mannitol (1:1 w/w), or NaCl (1:1 w/w) or in the absence of excipients. Immediately after lyophilization, samples of each formulation were analyzed by ssHDX-MS and Fourier transform infrared spectroscopy (FTIR) to assess Mb conformation, and by dynamic light scattering (DLS) and size exclusion chromatography (SEC) to determine the extent of aggregation. The remaining samples were then placed on stability at 25 °C and 60% RH or 40 °C and 75% RH for up to 1 year, withdrawn at intervals, and analyzed for aggregate content by SEC and DLS. In ssHDX-MS of samples immediately after lyophilization (t = 0), Mb was less deuterated in solids containing sucrose (1:1 and 1:8 w/w) than in those containing mannitol (1:1 w/w), NaCl (1:1 w/w), or Mb alone. Deuterium uptake kinetics and peptide mass envelopes also indicated greater Mb structural perturbation in mannitol, NaCl, or Mb-alone samples at t = 0. The extent of deuterium incorporation and kinetic parameters related to rapidly and slowly exchanging amide pools (Nfast, Nslow), measured at t = 0, were highly correlated with the extent of aggregation on storage as measured by SEC. In contrast, the extent of aggregation was weakly correlated with FTIR band intensity and peak position measured at t = 0. The results support the use of ssHDX-MS as a formulation screening tool in developing lyophilized protein drug products.

Eihän "leikkitäti" voi väsyä, vai voiko? : Käytännön olosuhteet ja työstä aiheutuva stressi musiikkileikkikoulunopettajan työssä

OpenAIRE

Busk, Vilma

2012-01-01

TIIVISTELMÄ Tässä opinnäytetyössä tarkastellaan musiikkileikkikoulunopettajan työtä ja sen haasteita. Keskiössä ovat työn käytännön olosuhteet ja työstä aiheutuva stressi. Opinnäytetyötäni varten tein kyselytutkimuksen, jonka toteutin Internet-kyselynä Facebookissa, Muskari-ideoita-sivustolla. Kyselyyn vastasi 48 musiikkileikkikoulunopettajaa, joilla oli vaihteleva määrä kokemusta ja jotka olivat iältään 21—50-vuotiaita. Vastaajien taustatietojen lisäksi kartoitin mahdollisimman mon...

A PIONIER and Incisive Look at the Interacting Binary SS Lep

Science.gov (United States)

Blind, N.; Boffin, H. M. J.; Berger, J.-P.; Lebouquin, J.-B.; Mérand, A.

2011-09-01

Symbiotic stars are excellent laboratories to study a broad range of poorly understood physical processes, such as mass loss of red giants, accretion onto compact objects, and evolution of nova-like outbursts. As their evolution is strongly influenced by the mass transfer episodes, understanding the history of these systems requires foremost to determine which process is at play: Roche lobe overflow, stellar wind accretion, or some more complex mixture of both. We report here an interferometric study of the symbiotic system SS Leporis, performed with the unique PIONIER instrument. By determining the binary orbit and revisiting the parameters of the two stars, we show that the giant does not fill its Roche lobe, and that the mass transfer most likely occurs via the accretion of an important part of the giant's wind.

Osakeyhtiön sivuuttaminen nykyisessä tuloverotuskäytännössä

OpenAIRE

Kuha, Pia

2015-01-01

Opinnäytetyön tarkoituksena oli tutkia mikä on nykyinen käytäntö osakeyhtiöiden sivuuttamisissa verotuskäytännössä sekä mihin suuntaan tulevaisuudessa osakeyhtiöiden sivuuttamiskäytäntö on mahdollisesti muuttumassa. Idea opinnäytetyöhön lähti ollessani työharjoittelussa syksyllä 2013 toimeksiantajan kanssa käydyistä keskusteluista ja omasta kiinnostuksesta verotuskäytäntöihin. Toimeksiantaja on oululainen taloushallinnon palveluita tarjoava yritys, joka on vuodesta 2011 tarjonnut laadukkaita ...

Ikääntyneiden seksuaalisuus ja sen tukeminen hoitotyössä - ohjeita hoitajille

OpenAIRE

Hoffrén, Jenni; Eskelinen, Noora

2014-01-01

Tämän opinnäytetyön tarkoituksena on kuvata ikääntyneiden seksuaalisuutta ja kuinka hoitajat voivat tukea seksu-aalisuutta hoitotyössä. Opinnäytetyön tavoitteena on luoda opas ikääntyneiden seksuaalisuuden tukemiseksi Leivolan hoitohenkilökunnalle, johon kuuluu perus-, lähi- ja sairaanhoitajia. Toimeksiantajanamme on Leivolan palvelukeskus Leppävirralla, joka on ympärivuorokautinen hoitokoti. Palvelukeskuksen asukkaiden ikäjakauma on 63–99 vuotta. Opinnäytetyö on kehittämistyö. Opinnäyte...

VizieR Online Data Catalog: Photometric variability of BeSS-KELT stars (Labadie-Bartz+, 2017)

Science.gov (United States)

Labadie-Bartz, J.; Pepper, J.; McSwain, M. V.; Bjorkman, J. E.; Bjorkman, K. S.; Lund, M. B.; Rodriguez, J. E.; Stassun, K. G.; Stevens, D. J.; James, D. J.; Kuhn, R. B.; Siverd, R. J.; Beatty, T. G.

2018-04-01

Kilodegree Extremely Little Telescope (KELT) is a photometric survey using two small-aperture (42 mm) wide-field (26°x26°) telescopes, with a northern location at Winer Observatory in Arizona in the United States, and a southern location at the South African Astronomical Observatory near Sutherland, South Africa. The Be Star Spectra (BeSS) database is a continually updated catalog that attempts to include all known Be stars, as well as their stellar parameters. This catalog is based primarily on the catalog of classical Be stars published by Jaschek et al. (1982, Cat. III/67) but also includes more recently discovered Be stars from a variety of sources (e.g., Neiner et al. 2005ApJS..156..237N; Martayan et al. 2006A&A...445..931M). From the BeSS database, we compiled a list of all the classical Be stars with 7

Conducta alimentaria y perfil glucémico en dos líneas de ratas con diabetes genética: eSS y eSMT

OpenAIRE

Montenegro, Silvana Marisa; Tarrés, María Cristina; Picena, Juan Carlos; Martínez, Stella Maris

2005-01-01

Introducción. La alimentación puede agravar la diabetes de humanos y modelos animales. Entre ellos, la rata eSMT presenta un curso más exacerbado y mayor biomasa que la línea parental eSS. Objetivo. Estudiar la conducta alimentaria de ratas macho eSMT y eSS ante oferta ilimitada y bajo restricción alternada, analizando su incidencia sobre el peso y la glucemia. Materiales y métodos. Se registró el consumo ad libitum en etapas de crecimiento y mantenimiento. Un grupo de eSMT recibió durante 5 ...

Conducta alimentaria y perfil glucémico en dos líneas de ratas con diabetes genética: eSS y eSMT.

OpenAIRE

Silvana Marisa Montenegro; María Cristina Tarrés; Juan Carlos Picena; Stella Maris Martínez

2005-01-01

Introducción. La alimentación puede agravar la diabetes de humanos y modelos animales. Entre ellos, la rata eSMT presenta un curso más exacerbado y mayor biomasa que la línea parental eSS. Objetivo. Estudiar la conducta alimentaria de ratas macho eSMT y eSS ante oferta ilimitada y bajo restricción alternada, analizando su incidencia sobre el peso y la glucemia. Materiales y métodos. Se registró el consumo ad libitum en etapas de crecimiento y mantenimiento. Un grupo de eSMT recibió durante 5 ...

"...Aikaa kohdata rauhassa lapsi..." : Päivähoidossa työskentelevien kokemuksia vertaisryhmässään sivuun jäävistä lapsista

OpenAIRE

Kaiponen, Jaana; Koskela, Jenna

2009-01-01

Kaiponen, Jaana & Koskela, Jenna. ”…Aikaa kohdata rauhassa lapsi…” - Päivähoidossa työskentelevien kokemuksia vertaisryhmässään sivuun jäävistä lapsista. Oulu, syksy 2009, 66 s., 2 liitettä. Diakonia-ammattikorkeakoulu, Diak Pohjoinen Oulu. Sosiaalialan koulutusohjelma, Diakonisen sosiaalityön suuntautumisvaihtoehto, sosionomi (AMK) + diakonin virkakelpoisuus. Opinnäytetyön tarkoituksena oli kuvata päivähoidon työntekijöiden kokemuksia vertaisryhmässään sivuun jäävistä lapsista. Tavoittee...

Hypothalamic expression of KiSS-1 system and gonadotropin-releasing effects of kisspeptin in different reproductive states of the female Rat.

Science.gov (United States)

Roa, J; Vigo, E; Castellano, J M; Navarro, V M; Fernández-Fernández, R; Casanueva, F F; Dieguez, C; Aguilar, E; Pinilla, L; Tena-Sempere, M

2006-06-01

Kisspeptins, products of the KiSS-1 gene with ability to bind G protein-coupled receptor 54 (GPR54), have been recently identified as major gatekeepers of reproductive function with ability to potently activate the GnRH/LH axis. Yet, despite the diversity of functional states of the female gonadotropic axis, pharmacological characterization of this effect has been mostly conducted in pubertal animals or adult male rodents, whereas similar studies have not been thoroughly conducted in the adult female. In this work, we evaluated maximal LH and FSH secretory responses to kisspeptin-10, as well as changes in sensitivity and hypothalamic expression of KiSS-1 and GPR54 genes, in different physiological and experimental models in the adult female rat. Kisspeptin-10 (1 nmol, intracerebroventricular) was able to elicit robust LH bursts at all phases of the estrous cycle, with maximal responses at estrus; yet, in diestrus LH, responses to kisspeptin were detected at doses as low as 0.1 pmol. In contrast, high doses of kisspeptin only stimulated FSH secretion at diestrus. Removal of ovarian sex steroids did not blunt the ability of kisspeptin to further elicit stimulated LH and FSH secretion, but restoration of maximal responses required replacement with estradiol and progesterone. Finally, despite suppressed basal levels, LH and FSH secretory responses to kisspeptin were preserved in pregnant and lactating females, although the magnitude of LH bursts and the sensitivity to kisspeptin were much higher in pregnant dams. Interestingly, hypothalamic KiSS-1 gene expression significantly increased during pregnancy, whereas GPR54 mRNA levels remained unaltered. In summary, our current data document for the first time the changes in hypothalamic expression of KiSS-1 system and the gonadotropic effects (maximal responses and sensitivity) of kisspeptin in different functional states of the female reproductive axis. The present data may pose interesting implications in light of the

Cherenkov and scintillation light separation on the CheSS experiment

Science.gov (United States)

Caravaca, Javier; Land, Benjamin; Descamps, Freija; Orebi Gann, Gabriel D.

2016-09-01

Separation of the scintillation and Cherenkov light produced in liquid scintillators enables outstanding capabilities for future particle detectors, the most relevant being: particle directionality information in a low energy threshold detector and improved particle identification. The CheSS experiment uses an array of small, fast photomultipliers (PMTs) and state-of-the-art electronics to demonstrate the reconstruction of a Cherenkov ring in liquid scintillator using two techniques: based on the photon density and using the photon hit time information. A charged particle ionizing a scintillation medium produces a prompt Cherenkov cone and late isotropic scintillation light, typically delayed by several ns. The fast response of our PMTs and DAQ provides a precision well below the ns level, making possible the time separation. Furthermore, the usage of the new developed water-based liquid scintillators (WbLS) enhances the separation since it allows tuning of the Cherenkov/Scintillation ratio. Latest results on the separation for pure liquid scintillators and WbLS will be presented.

Optical, UV, and EUV Oscillations of SS Cygni in Outburst

Science.gov (United States)

Mauche, Christopher W.

2004-07-01

I provide a review of observations in the optical, UV (HST), and EUV (EUVE and Chandra LETG) of the rapid periodic oscillations of nonmagnetic, disk-accreting, high mass-accretion rate cataclysmic variables (CVs), with particular emphasis on the dwarf nova SS Cyg in outburst. In addition, I drawn attention to a correlation, valid over nearly six orders of magnitude in frequency, between the frequencies of the quasi-periodic oscillations (QPOs) of white dwarf, neutron star, and black hole binaries. This correlation identifies the high frequency quasi-coherent oscillations (so-called ``dwarf nova oscillations'') of CVs with the kilohertz QPOs of low mass X-ray binaries (LMXBs), and the low frequency and low coherence QPOs of CVs with the horizontal branch oscillations (or the broad noise component identified as such) of LMXBs. Assuming that the same mechanisms produce the QPOs of white dwarf, neutron star, and black hole binaries, this correlation has important implications for QPO models.

Evaluating the effects of hydroxyapatite coating on the corrosion behavior of severely deformed 316Ti SS for surgical implants

International Nuclear Information System (INIS)

Mhaede, Mansour; Ahmed, Aymen; Wollmann, Manfred; Wagner, Lothar

2015-01-01

The present work investigates the effects of severe plastic deformation by cold rolling on the microstructure, the mechanical properties and the corrosion behavior of austenitic stainless steel (SS) 316Ti. Hydroxyapatite coating (HA) was applied on the deformed material to improve their corrosion resistance. The martensitic transformation due to cold rolling was recorded by X-ray diffraction spectra. The effects of cold rolling on the corrosion behavior were studied using potentiodynamic polarization. The electrochemical tests were carried out in Ringer's solution at 37 ± 1 °C. Cold rolling markedly enhanced the mechanical properties while the electrochemical tests referred to a lower corrosion resistance of the deformed material. The best combination of both high strength and good corrosion resistance was achieved after applying hydroxyapatite coating. - Highlights: • Cold rolling markedly increases the hardness of SS 316Ti from 125 to 460 HV10. • Higher deformation degrees lead to lower corrosion resistance. • Application of HA-coating leads to significant improvement of the corrosion resistance

Digitaalisen markkinoinnin keinoja internetin vuorovaikutteisessa ympäristössä : markkinointiresurssien suuntaaminen internetissä

OpenAIRE

Ellonen, Niko

2013-01-01

Tämä opinnäytetyö keskittyy digitaaliseen markkinointiin ja markkinointiresurssien suuntaamiseen internetin vuorovaikutteisessa ympäristössä. Markkinointi on myynnin kasvua tukeva toiminto, jonka tavoitteena on luoda ja tuottaa asiakkaille arvoa ja hallita asiakassuhteita tavoilla, jotka hyödyttävät yritystä ja sen sidosryhmiä. Digitaalinen markkinointi on digitaalisessa muodossa tai mediassa tehtyä markkinointia. Tutkimuksen alussa käsitellään ensin yleisesti markkinoinnin määritelmää ...

Role of ethanol on growth, laccase production and protease activity in Pycnoporus cinnabarinus ss3

OpenAIRE

Meza, Juan Carlos; Auria, Richard; Lomascolo, A.; Sigoillot, J. C.; Casalot, Laurence

2007-01-01

Laccase production by the strain Pycnoporus cinnabarinus ss3 was studied in a solid-state culture on sugar-cane bagasse using chemical compounds as inducers (ethanol, methanol, veratryl alcohol and ferulic acid). Laccase productions were about 5- to 8.5-fold higher than non-induced cultures. Liquid-culture experiments with "Glabeled ethanol were conducted. Ninety-eight percent of the initial amount of C-14 from ethanol was recovered as (CO2)-C-14, C-14-biomass and soluble C-14-compounds (main...

Combining use of a panel of ssDNA aptamers in the detection of Staphylococcus aureus

OpenAIRE

Cao, Xiaoxiao; Li, Shaohua; Chen, Liucun; Ding, Hongmei; Xu, Hua; Huang, Yanping; Li, Jie; Liu, Nongle; Cao, Weihong; Zhu, Yanjun; Shen, Beifen; Shao, Ningsheng

2009-01-01

In this article, a panel of ssDNA aptamers specific to Staphylococcus aureus was obtained by a whole bacterium-based SELEX procedure and applied to probing S. aureus. After several rounds of selection with S. aureus as the target and Streptococcus and S. epidermidis as counter targets, the highly enriched oligonucleic acid pool was sequenced and then grouped under different families on the basis of the homology of the primary sequence and the similarity of the secondary structure. Eleven sequ...

Uudistuva johtajuus ja Z-sukupolvi työelämässä

OpenAIRE

Aakkula, Eveliina

2012-01-01

Opinnäytetyössä käsitellään epäjohtamista, joka on uusi johtamisen käsite, jonka ovat luoneet Janne Tienari ja Rebekka Piekkari kirjassaan Z ja epäjohtaminen. Terminä epäjohtaminen ei ole virallinen, eikä tule välttämättä vakiintumaan johtajuuden käsitteiden joukkoon. Tämä uusi johtamisen malli liitetään vahvasti Z-sukupolven tulemiseen työmarkkinoille. Z-sukupolvea ovat 1990-luvulla syntyneet. Johtajuus on tärkeä aihe, jonka käsittelemiseen löytyy aina uusia näkökulmia, joiden toimivuutta vo...

Enhanced photocathodic protection performance of Ag/graphene/TiO2 composite for 304SS under visible light

Science.gov (United States)

Li, Hong; Wang, Xiutong; Wei, Qinyi; Liu, Xueqing; Qian, Zhouhai; Hou, Baorong

2017-06-01

Ag and graphene co-sensitized TiO2 composites were successfully fabricated and used as photoanodes for photogenerated cathodic protection of 304 stainless steel (304SS) under visible light. Graphene films was firstly deposited onto the TiO2 nanotube (NT) films via cyclic voltammetric electrodeposition. Ag/graphene/TiO2 films were then fabricated via dipping and photoreduction method. The morphology, composition and optical response of the Ag/graphene/TiO2 NT composites were characterized by scanning electron microscopy, x-ray diffraction, x-ray photoelectron spectroscopy, UV-vis diffusion reflectance spectroscopy, respectively. The photocathodic protection performance of the Ag/graphene/TiO2 composites were systematically studied through open-circuit potential and potentiodynamic polarization measurements in 3.5 wt% NaCl solution under visible light (λ > 400 nm). The composites exhibited enhanced photogenerated cathodic protection performance for 304SS under visible light irradiation compared to pure TiO2. Graphene and Ag have a synergistic effect on the enhancement of photocathodic protection performance of TiO2. The composites prepared with 30-cycle graphene film and 15 mM AgNO3 solution showed the optimal corrosion protection performance.

Corrosion behavior of mild steel and SS 304L in presence of dissolved nickel under aerated and deaerated conditions

Directory of Open Access Journals (Sweden)

Mohd Mobin

2011-12-01

Full Text Available In dual purpose water/power co-generation plants, the presence of high concentration of Cu and Ni in the re-circulating brine/condensate as a result of condenser tubes corrosion has been attributed as one of the several causes of corrosion damage of flash chamber materials and water touched parts of the boilers. The present investigation deals with the effect of dissolved nickel in the concentration range of 10 ppb to 100 ppm on the corrosion behavior of mild steel and SS 304L in two aqueous medium namely, distilled water and artificial seawater. The effect of pH, dissolved oxygen and flow condition of aqueous medium on the corrosion behavior was also monitored. The experimental techniques include immersion test and electrochemical tests which include free corrosion potential measurements and potentiodynamic polarization measurements. The corrosion rate of mild steel and SS 304L under different experimental conditions was determined by weight loss method and spectrophotometric determination of iron ion entered into the test solution during the period of immersion. The pH of the test solution was also monitored during the entire period of immersion. The left over nickel ions present in the test solution after completion of immersion was also estimated using Atomic Absorption Spectrophotometer. The surface morphology of the corroded steel surface was also examined using scanning electron microscopy (SEM. The results of the studies show that SS 304L largely remains unaffected in both distilled water and artificial seawater under different experimental conditions. However, the effect of nickel on the corrosion behavior of mild steel is quite pronounced and follows interesting trends.

Totally extraperitoneal (TEP) bilateral hernioplasty using the Single Site® robotic da Vinci platform (DV-SS TEP): description of the technique and preliminary results.

Science.gov (United States)

Cestari, A; Galli, A C; Sangalli, M N; Zanoni, M; Ferrari, M; Roviaro, G

2017-06-01

Laparoendoscopic single site totally extraperitoneal (TEP) hernia repair showed to be a feasible alternative to conventional laparoscopic hernia repair; nevertheless single site surgery, with the loss of instruments triangulation can be a demanding procedure. To overcome those hurdles, the Single Site® (SS) platform of the da Vinci (DV) Si robotic system enables to perform surgical procedures through a 25-mm skin incision, with a stable 3D vision and restoring an adequate triangulation of the surgical instruments. We present in details the technique and the preliminary results of DV-SS TEP, to our knowledge the first cases reported in literature. In March 2016, three consecutive male patients (mean age 46.6 years-mean BMI 25.3) with bilateral symptomatic inguinal hernia were submitted to DV-SS TEP in our institutions. Feasibility, codification of the technique, operative time and perioperative outcomes were recorded. All the procedures were completed as scheduled, with no conversion to other techniques. Mean operative time was 98.6 min, ranging between 155 and 55 min, reflecting the learning curve of the operating room team on this new procedure. No intraoperative or postoperative complications were experienced and all the patients were discharged within 24 h after surgery. Patients reported satisfactory postoperative course, with no recurrence of inguinal hernia and satisfaction in cosmetic result at 6-month follow-up. DV-SS TEP inguinal hernia repair showed to be feasible and effective surgical option for bilateral groin hernia repair. Patients' outcome was uneventful, with optimal cosmetic results. Further studies comparing this innovative technique to TEP or LESS TEP should be promoted.

Liiketalouden koulutusohjelman alumnisuhteiden kehittäminen HAAGA-HELIA:n Porvoon yksikössä

OpenAIRE

Nguyen, Ainu

2014-01-01

Opinnäytetyön tarkoitus on kehittää liiketalouden koulutusohjelman suorittaneiden alumnien verkostoa HAAGA-HELIA:n Porvoon yksikössä. Tutkimuksen tehtävänä oli selvittää alumnien mielipiteitä, joiden avulla pystytään kehittämään heille parhaiten so-veltuvia alumnitoimintoja sekä yhteistyömuotoja, joita myös Porvoon yksikkö pystyy hyödyntämään. Alumniverkoston avulla pidetään paremmin yhteyttä alumnien ja HAAGA-HELIA:n Porvoon yksikön välillä. Teoriaosuus koostuu alumnitoiminnan käsitt...

Interaction of dense nitrogen plasma with SS304 surface using APF plasma focus device

Science.gov (United States)

Afrashteh, M.; Habibi, M.; Heydari, E.

2012-04-01

The nitridation of SS304 surfaces is obtained by irradiating nitrogen ions from Amirkabir plasma focus device, which use multiple focus deposition shots at optimum distance 10 cm from the anode. The Vickers Micro-Hardness values are improved more than twice for the nitrided samples comparing to the nonnitrided ones. The X-ray diffraction (XRD) analysis is carried out in order to explore the phase changes in the near surface structure of the metals. The results of Scanning Electron Microscopy (SEM) indicate changes in surface morphology which are the emergence of smooth and uniform film on the surface of the nitrided metals.

Osallisena omassa elämässä : Kehitysvammaisen nuoren itsenäistymisen tukeminen

OpenAIRE

Rosbacka, Marjo

2009-01-01

Opinnäytetyö tehtiin yhteistyössä Eskoon aikuiskoulutusyksikkö Kaarisillan kanssa. Tutkinnan kohteena oli kehitysvammaisten nuorten itsenäisyys ja osallisuus arkipäivän toimintaan. Työn tarkoituksena on herättää ihmisiä pohtimaan tasa-arvoista elämää. Mitä siihen kuuluu ja kenen tehtävänä on varmistaa yhdenvertaisuus? Tutkimuksen pääongelmaksi muodostui; Miten tavallisen nuoren ja kehitysvammaisen nuoren elämät eroavat toisistaan? Lisäksi tutkimuksessa selvitettiin, miksi elämät eroavat toisi...

Treatment of Petroleum Sludge By Using Solidification/Stabilization (S/S) Method : Effect of Hydration Days to Heavy Metals Leaching and Strength

Science.gov (United States)

Murshid, N.; Kamil, N. A. F. M.; Kadir, A. A.

2018-04-01

Petroleum sludge is one of the major solid wastes generated in the petroleum industry. Generally, there are numbers of heavy metals in petroleum sludge and one treatment that is gaining prominence to treat a variety of mixed organic and inorganic waste is solidification/stabilization (S/S) method. The treatment protects human health and the environment by immobilizing contaminants within the treated material and prevents migration of the contaminants. In this study, solidification/stabilization (S/S) method has been used to treat the petroleum sludge. The comparison of hydration days, namely, 7th and 28th days in these cement-based waste materials were studied by using Synthetic Precipitate Leaching Procedure (SPLP). The results were compared to the United States Environmental Protection Agency (USEPA) standards. The results for leaching test concluded that less percentage OPC gave maximum concentration of heavy metals leaching due to deficient in Calcium Oxide (CaO), which is can caused weak solidification in the mixture. Physical and mechanical properties conducted such as compressive strength and density test. From the results, it shows addition up to of 30percentage PS give results which comply with minimum landfill dispose limit. The results shows correlation between strength and density are strong regression coefficient of 82.7%. In conclusion, S/S method can be alternative disposal method for PS in the same time complies with standard for minimum landfill disposal limit. The results for leaching test concluded the less OPC percentage gave maximum concentration of heavy metals leaching.

Pulsed laser deposited MnCo{sub 2}O{sub 4} protective layer on SS430 for solid oxide fuel cell application

Energy Technology Data Exchange (ETDEWEB)

Gaur, Anshu, E-mail: gauranshu20@gmail.com, E-mail: ahamed.vza@gmail.com; Mohiddon, Md. Ahamad, E-mail: gauranshu20@gmail.com, E-mail: ahamed.vza@gmail.com [National Institute of Technology Andhra Pradesh, Tadepalliguem 534101 (India); Prasad, Muvva D. [UGC Networking Centre, School of Chemistry, University of Hyderabad, Hyderabad 500046, India. Phone:+91-40-23134382, Fax:+91-40-23010227 (India)

2016-05-23

The growth and oxidation study of pulsed laser deposited MnCo{sub 2}O{sub 4} protective layer on SS430 substrate for solid oxide fuel cell application is demonstrated. MnCo{sub 2}O{sub 4} has been achieved in three different ways including, deposition at higher substrate temperature (700°C), and deposition at room temperature on pre-oxidized and untreated SS430 substrate followed by annealing at 700°C for 2 hrs. X-ray diffraction and Raman spectroscopy has been applied to demonstrate the kind of phases developed in each case. These three samples were subjected to heat treatment at 750°C for 5 hr. The extent of undesired Fe{sub 2}O{sub 3} phase formation in the post deposition heat treated samples is discussed based on Raman spectroscopic results.

The genetics and ecology of male reproductive investments in the malaria mosquito Anopheles gambiae s.s

OpenAIRE

Ekechukwu, Nkuru Esther

2015-01-01

Malaria continues to be a major global health problem due to high mortality and morbidity rate in endemic regions. An. gambiae s.s is the major vector in endemic African countries. About 198 million cases of malaria were recorded globally in 2013 and this have led to over 584 000 deaths. Different measures have been implemented in order to reduce and control the transmission rate. However, the drug resistant parasites and insecticide resistant mosquitoes have created problems towards achievin...

Pulsar-driven Jets In Sne, Grbs, Lmxbs, Ss 433, And The Universe

Science.gov (United States)

Middleditch, John

2011-05-01

The model of pulsar emission through superluminally induced polarization currents, (SLIP), predicts that pulsations produced by such currents at many light cylinder radii by a rotating, magnetized body, will drive pulsations close to the axis of rotation. In SN 1987A, the possible Rosetta Stone for 99% of SNe, GRBs, ms pulsars, and SS 433, such highly collimated (>1 in 10,000) 2.14 ms pulsations, and the similarly collimated jets of particles which they drove, including 1e-6 solar masses with velocities 0.95 c, were responsible for its very early light curve (days 3-20), its "Mystery Spot," observed slightly later (0.5 to 0.3 c, at days 30-50 and after), and still later, in less collimated form, its bipolarity. The axially driven pulsations enforce a toroidal geometry onto all early SNRs, rendering even SNe Ia unsuitable as standard candles. The numbers for Sco X-1's jet are identical, while those for SS 433 are lower (0.26 c), because of the absence of velocity "boosting" via collisions of heavy elements with lighter ones, due to the nearly pure hydrogen content of the supercritical accretion. SLIP also drives positrons from SNe to high energies, possibly accounting for the excess seen by PAMELA at scores of GeV, and predicts that almost all pulsars with very sharp single pulses have been detected because the Earth is in a favored direction where their fluxes diminish only as 1/distance, and this has been verified in the laboratory as well as for the Parkes Multibeam Survey. SLIP also predicts that GRB afterglows will be 100% pulsed at 500 Hz in their proper frame. Finally, SLIP jets from SNe of the first stars may allow galaxies to form without the need for dark matter. This work was supported in part by the Department of Energy through the Los Alamos Directed Research Grant DR20080085.

SS Cygni: The accretion disk in eruption and at minimum light

International Nuclear Information System (INIS)

Kiplinger, A.L.

1979-01-01

Absolute spectrophotometric observations of the dwarf nova SS Cygni have been obtained at maximum light, during the subsequent decline, and at minimum light. In order to provide a critical test of accretion disk theory, a model for a steady-state α-model accretion disk has been constructed which utilizes a grid of stellar energy distributions to synthesize the disk flux. Physical parameters for the accretion disk at maximum light are set by estimates of the intrinsic luminosity of the system that result from a desynthesis of a composite minimum light energy distribution. At maximum light, agreements between observational and theoretical continuum slopes and the Balmer jump are remarkably good. The model fails, however, during the eruption decline and at minimum light. It appears that the physical character of an accretion disk at minimum light must radiacally differ from the disk observed at maximum light

Why complexity matters: using ALMaSS for risk assessment of wildlife

DEFF Research Database (Denmark)

Topping, Christopher John

with these system properties is agentbased modelling (ABM).ABMs are capable of integrating a range of drivers and actors in space and time and can represent detailed farming operations on a large scale, integrating these with realistic models of animal populations, and expressing each animal as an individual agent...... in time and space. All of these factors can affect the risk assessment. There is also another, difficult to observe, property of real systems, and that is the spatio-temporal dynamics associated with populations, climate, management, and ecology and behaviour, and the potential for feedback loops......-sink dynamics are emergent properties and do not need to be imposed.ALMaSS is one such model system and is used here to exemplify aspects of complexity in population-level risk assessment for terrestrial mammals, birds, and arthropods exposed to pesticides. Results are used to argue for greater realism...

[Factorial structure and psychometric criteria of the German translation of the Maslach Burnout Inventory--Student Version by Schaufeli et al. (MBI-SS)].

Science.gov (United States)

Gumz, Antje; Erices, Rainer; Brähler, Elmar; Zenger, Markus

2013-02-01

The Maslach Burnout Inventory (MBI) is the most commonly used instrument in research on burnout. For the German translation of the MBI student version (MBI-SS), the postulated 3-factorial structure of the questionnaire could be confirmed using confirmatory analyses. The internal consistencies of the scales can be classified as good. First findings underline the construct validity of the questionnaire. As expected, burnout was associated with psychic and somatic complaints as well as with experienced social support. Couples reported higher "Efficiency" levels. Academic studies became less important with increasing duration. Time pressure during the last month was correlated with "Exhaustion". The presented findings on factorial structure and validity speak for the applicability of MBI-SS for research projects on students of German institutes of higher education. © Georg Thieme Verlag KG Stuttgart · New York.

Evolution of microstructure in laser welding of SS304L

International Nuclear Information System (INIS)

Kumar, Santosh; Kushwaha, R.P.; Viswanadham, C.S.; Dey, G.K.

2009-01-01

Laser welding is an important joining process and its application in industries is growing rapidly. One can produce laser welds over a wide range of process parameters and this offers very good opportunity for producing microstructure of different morphology and scales in the weldment. Weld beads have been produced on 5 mm thick plates of SS304L using CW Nd-YAG laser. Laser power was varied in 200 W to 1000 W range and welding speed was varied in 100 mm/mm to 1000 mm/mm. This resulted in weld beads of different morphology. Microstructure of the weld beads was examined on the cross-section as well as in the axial direction using optical microscopy and Transmission Electron Microscopy (TEM) to study evolution of the microstructure in the weldment. Microstructure was cellular and cellular-dendritic with grains growing from the fusion line towards the centerline. In the central region, cellular growth along the welding direction was observed. The cell size was found to increase with increasing laser power and decreasing welding speed. The findings are presented in this paper. (author)

Key steps in type III secretion system (T3SS) towards translocon assembly with potential sensor at plant plasma membrane.

Science.gov (United States)

Ji, Hongtao; Dong, Hansong

2015-09-01

Many plant- and animal-pathogenic Gram-negative bacteria employ the type III secretion system (T3SS) to translocate effector proteins from bacterial cells into the cytosol of eukaryotic host cells. The effector translocation occurs through an integral component of T3SS, the channel-like translocon, assembled by hydrophilic and hydrophobic proteinaceous translocators in a two-step process. In the first, hydrophilic translocators localize to the tip of a proteinaceous needle in animal pathogens, or a proteinaceous pilus in plant pathogens, and associate with hydrophobic translocators, which insert into host plasma membranes in the second step. However, the pilus needs to penetrate plant cell walls in advance. All hydrophilic translocators so far identified in plant pathogens are characteristic of harpins: T3SS accessory proteins containing a unitary hydrophilic domain or an additional enzymatic domain. Two-domain harpins carrying a pectate lyase domain potentially target plant cell walls and facilitate the penetration of the pectin-rich middle lamella by the bacterial pilus. One-domain harpins target plant plasma membranes and may play a crucial role in translocon assembly, which may also involve contrapuntal associations of hydrophobic translocators. In all cases, sensory components in the target plasma membrane are indispensable for the membrane recognition of translocators and the functionality of the translocon. The conjectural sensors point to membrane lipids and proteins, and a phosphatidic acid and an aquaporin are able to interact with selected harpin-type translocators. Interactions between translocators and their sensors at the target plasma membrane are assumed to be critical for translocon assembly. © 2014 BSPP AND JOHN WILEY & SONS LTD.

RICOMPOSIZIONE VIRTUALE DEL TABERNACOLO DI ISAIA DA PISA PER LA CHIESA DELLA SS. TRINITÀ DI VITERBO

Directory of Open Access Journals (Sweden)

Dante Abate

2012-04-01

Full Text Available

Questo progetto ha preso spunto da uno studio condotto da Giusy Zevolini e pubblicato nel 2003 sulla Rivista dell’Istituto Nazionale di Archeologia e Storia dell’Arte. Nell’articolo la studiosa proponeva un’ipotesi di ricomposizione di due opere scultoree in marmo, entrambe eseguite durante il XV secolo, una delle quali sicuramente dall’artista toscano Isaia da Pisa. Le due parti, in origine probabilmente inserite in un unico complesso scultoreo monumentale, all’interno della chiesa agostiniana della SS. Trinità di Viterbo, oggi sono conservate in due siti distinti del capoluogo del territorio della Tuscia.

Virtual Reconstruction of the Tabernacolo of Isaia da Pisa for the Church of SS. Trinità in Viterbo

This study is based upon a characteristic, Art History paper, published in 2003. Specifically it suggests a reconstruction hypotesis concerning two marble objects, created in the XV Century, which were supposed to belong to each other, in a monumental complex, inside the Augustinian Church of the SS. Trinità in Viterbo (Italy. In the article the iconography was rendered by simply stitching pictures together, losing the objects proportions, without any care given to the likely missing parts, and of course showing the image in 2D. Despite scholars mostly agreeing that the two marble artefacts were made by the same artist (Isaiada Pisa and that both belong to the same church, they are still notsure if the pieces are a unique structure. The virtual re-composition helps to enhance the visual perception of the whole construction, which is now available just in 2D flat images, and to understand how it originally would have looked. The two objects, today placed in different locations in the city of Viterbo, studied in this paper are: a marble tabernacle with Angels and Christ; a marble tympanum with a blessing by Christ. Both sculptures were acquired witha triangulation laser scanner in two different

Construct validity of the items on the Stroke Specific Quality of Life (SS-QOL) questionnaire that evaluate the participation component of the International Classification of Functioning, Disability and Health.

Science.gov (United States)

Silva, Soraia Micaela; Corrêa, Fernanda Ishida; Pereira, Gabriela Santos; Faria, Christina Danielli Coelho de Morais; Corrêa, João Carlos Ferrari

2018-01-01

Analyze the construct validity and internal consistency of the Stroke Specific Quality of Life (SS-QOL) items that address the participation component of the ICF as well as analyze the ceiling and floor effects. One hundred subjects were analyzed: 85 community-dwelling and 15 institutionalized individuals. The analysis of construct validity was performed using classic psychometrics: (1) the comparison of known groups (individuals without restriction to participation vs. those with restriction to participation) using the Mann-Whitney test and (2) convergent validity - correlation between the scores on the SS-QOL items that address participation and the subscale scores of measures used to evaluate the similar constructs and concepts [the Short-Form Health Survey (SF-36), Functional Independence Measure (FIM) and grip strength test]. Spearman's correlation coefficients were calculated for this analysis. Cronbach's α was used for the analysis of internal consistency and both the ceiling and floor effects were analyzed. The level of significance for all analyses was α = 0.05. The a priori hypotheses regarding construct validity were partially demonstrated, as only five of the eight domains exhibited positive moderate to strong correlations (r > 0.40) with measures that address constructs similar to those addressed on the SS-QOL questionnaire. The items demonstrated adequate internal consistency and are capable of differentiating individuals with and without restriction to participation. The ceiling and floor effects were considered adequate for the total SS-QOL score, but beyond acceptable standards for some domains. The 26 items of the SS-QOL questionnaire measure a multidimensional construct and therefore do not only address participation. However, the items demonstrated adequate internal consistency and are capable of differentiating individuals with and without restriction to participation. Implications for rehabilitation The 26 items of the SS

Lysogenic Streptococcus suis isolate SS2-4 containing prophage SMP showed increased mortality in zebra fish compared to the wild-type isolate.

Directory of Open Access Journals (Sweden)

Fang Tang

Full Text Available Streptococcus suis (S. suis infection is considered to be a major problem in the swine industry worldwide. Based on the capsular type, 33 serotypes of S. suis have been described, with serotype 2 (SS2 being the most frequently isolated from diseased piglets. Little is known, however, about the pathogenesis and virulence factors of S. suis. Research on bacteriophages highlights a new area in S. suis research. A S. suis serotype 2 bacteriophage, designated SMP, has been previously isolated in our laboratory. Here, we selected a lysogenic isolate in which the SMP phage was integrated into the chromosome of strain SS2-4. Compared to the wild-type isolate, the lysogenic strain showed increased mortality in zebra fish. Moreover the sensitivity of the lysogenic strain to lysozyme was seven times higher than that of the wild-type.

Significance of measurement of serum somatostatin (SS), growth hormone (GH), insulin-like growth factor-1 (IGF-1) and some related cytokines levels in patients with graves' disease

International Nuclear Information System (INIS)

Zhu Bao; Ma Yunbao; Zhang Xiaoyi

2005-01-01

Objective: To study the possible roles of the related hormones and cytokines in the pathogenesis of Graves' disease. Methods: Serum levels of SS, GH, IGF-1, IL-2, IL-4 and IL-6 were measured with RIA in 1) 36 patients with Graves' disease before any treatment 2) another 30 patients with Graves' disease after successful anti-thyroid drug therapy and 3) 35 controls. Results: In the untreated group, the serum levels of SS, GH, IGF-1, IL-4 and IL-6 were significantly higher but serum IL-2 levels were significantly lower than those in the controls (P<0.01). In the successfully treated group, the serum levels of SS, GH, IGF-1, IL-4 and IL-6 were significantly lower (P<0.05 or P<0.01), but serum IL-2 levels were significantly higher (P<0.01) than those in the untreated group. Conclusion: These parameters played significant roles in the pathogenesis of Graves' disease; however, the precise mechanism remained to be speculative and further studies required. (authors)

Full Length Research Article

African Journals Online (AJOL)

Dr Ahmed

other areas of business administration. ... mathematics, real estate, insurance, actuarial science and business administration (McCutcheon & Scott, 1989). Most textbooks written in these ..... Mathematics of Finance; Heinemann; Oxford. Murray ...

Réponse équilibrée aux besoins en eau dans le bassin du Saïss au ...

International Development Research Centre (IDRC) Digital Library (Canada)

21 avr. 2011 ... L'équipe du projet qu'il dirige actuellement, axé sur la demande en eau dans le bassin du Saïss, tente de concilier les points de vue et les besoins très variés des intervenants. « Nos recherches se concentrent certes sur les agriculteurs, mais également sur les autres intervenants préoccupés par les ...

Use of the SS Scale, FIQR, and FIQ VASs for assessment of symptom severity in Egyptian fibromyalgia patients

OpenAIRE

Gihan M Omar; Shereen R Kamel; Rasha A Abdel-Magied; Nashwa M Abd-Allah

2014-01-01

Background Fibromyalgia (FM) is a complex syndrome associated with significant impairment in the quality of life and function. The ability to evaluate and measure the severity of FM is likely to provide several benefits. Objective This study aimed to assess symptom severity in Egyptian FM patients using the Symptom Severity Scale (SS Scale), Revised Fibromyalgia Impact Questionnaire (FIQR), and Fibromyalgia Impact Questionnaire Visual Analog Scales (FIQ VASs). Patients and method...

Polysaccharide Agaricus blazei Murill stimulates myeloid derived suppressor cell differentiation from M2 to M1 type, which mediates inhibition of tumour immune-evasion via the Toll-like receptor 2 pathway.

Science.gov (United States)

Liu, Yi; Zhang, Lingyun; Zhu, Xiangxiang; Wang, Yuehua; Liu, WenWei; Gong, Wei

2015-11-01

Gr-1(+) CD11b(+) myeloid-derived suppressor cells (MDSCs) accumulate in tumor-bearing animals and play a critical negative role during tumor immunotherapy. Strategies for inhibition of MDSCs are expected to improve cancer immunotherapy. Polysaccharide Agaricus blazei Murill (pAbM) has been found to have anti-cancer activity, but the underlying mechanism of this is poorly understood. Here, pAbM directly activated the purified MDSCs through inducing the expression of interleukin-6 (IL-6), IL-12, tumour necrosis factor and inducible nitric oxide synthase (iNOS), CD86, MHC II, and pSTAT1 of it, and only affected natural killer and T cells in the presence of Gr-1(+) CD11b(+) monocytic MDSCs. On further analysis, we demonstrated that pAbM could selectively block the Toll-like receptor 2 (TLR2) signal of Gr-1(+) CD11b(+) MDSCs and increased their M1-type macrophage characteristics, such as producing IL-12, lowering expression of Arginase 1 and increasing expression of iNOS. Extensive study showed that Gr-1(+) CD11b(+) MDSCs by pAbM treatment had less ability to convert the CD4(+) CD25(-) cells into CD4(+) CD25(+) phenotype. Moreover, result from selective depletion of specific cell populations in xenograft mice model suggested that the anti-tumour effect of pAbM was dependent on Gr-1(+ ) CD11b(+) monocytes, nether CD8(+) T cells nor CD4(+) T cells. In addition to, pAbM did not inhibit tumour growth in TLR2(-/-) mice. All together, these results suggested that pAbM, a natural product commonly used for cancer treatment, was a specific TLR2 agonist and had potent anti-tumour effects through the opposite of the suppressive function of Gr-1(+) CD11b(+) MDSCs. © 2015 John Wiley & Sons Ltd.

An assessment of post-remediation changes in sediment chemistry partitioning in an S/S treated soil.

Science.gov (United States)

Cutter, S.; MacLeod, C. L.; Canning, K.; Carey, P. J.; Hills, C. D.

2003-04-01

The Astra Pyrotechnics plant located in the Dartford Marshes, Kent, UK was the site of a field trial utilizing cement stabilization/solidification (S/S) in September 2000. A hotspot containing 35% copper and several thousand part per million lead and zinc was treated in order to trial an new form of cement stabilization (accelerated carbonation technology or ACT) developed by the Centre for Contaminated Land Remediation. A 10 by 20 meter plot was divided into 4 cells into which untreated, OPC (ordinary Portland cement) treated, Envirocem (a Lafarge special cement) treated and ACT treated soil was placed. Each cell has a leachate collection system and the leachate is monitored monthly. In August 2003, 24 cm cores were collected from each cell. These cores were analysed to determine changes in sediment chemistry and metal partitioning characteristics. Sediment chemistry was determined using an aqua regia digestion followed by ICP OES analysis. The CISMED extraction procedure was used to examine partitioning changes. The contaminant concentrations in the leachates remain below UK drinking water standards. However, seasonal cyclicity is observed with an increase of metals in the leachates during winter months. The sediment cores were analysed for Ca, Cr, Cu, Fe, K, Mn, Mg, Na, Ni, Pb and Zn. Only the untreated cell showed any discernable changes in metal concentration with depth. The S/S treated cells show no trends although differences in partitioning between the cells is observed.

Current Scientific Impact of Ss Cyril and Methodius University of Skopje, Republic of Macedonia in the Scopus Database (1960-2014).

Science.gov (United States)

Spiroski, Mirko

2015-03-15

The aim of this study was to analyze current scientific impact of Ss Cyril and Methodius University of Skopje, Republic of Macedonia in the Scopus Database (1960-2014). Affiliation search of the Scopus database was performed on November 23, 2014 in order to identify published papers from the Ss Cyril and Methodius University of Skopje (UC&M), Republic of Macedonia. A total number of 3960 articles (3055 articles from UC&M, 861 articles from Faculty of Medicine, UC&M, and 144 articles from Faculty of Pharmacy, UC&M) were selected for analysis (1960-2014). SCImago Journal Rank (SJR), Source Normalized Impact per Paper (SNIP) and h-index were calculated from the Scopus database. The number of published papers was sharply increased with maximum of 379 papers in 2012 year. The largest number of papers has been published in Macedonian Journal of Medical Sciences, Journal of Molecular Structure, Lecture Notes in Computer Science, Acta Pharmecutica, and Macedonian Journal of Chemistry and Chemical Engineering. The biggest SJR and SNIP has journal Nephrology Dialysis Transplantation. First three places of the top ten authors belong to Dimirovski GM, Gavrilovska L, and Gusev M. Top three places based on Scopus h-index (total number of published papers) belong to Kocarev L, Stafilov T, and Polenakovic M. The majority of papers originate from UC&M, but significant numbers of papers are affiliated to Faculty of Medicine, Faculty of Pharmacy, and Institute of Chemistry as members of UC&M, as well as Macedonian Academy of Sciences and Arts. Articles are the most dominant type of documents followed by conference papers, and review articles. Medicine is the most represented subject. Officials of the Ss Cyril and Methodius University of Skopje should undertake more effective and proactive policies for journal publishers and their Editorial Boards in order to include more journals from UC&M in the Scopus database.

Association analysis of polymorphism in KiSS1 gene with reproductive traits in goats.

Science.gov (United States)

El-Tarabany, Mahmoud S; Zaglool, Asmaa W; El-Tarabany, Akram A; Awad, Ashraf

2017-05-01

Understanding the genetic information of related genes is helpful for the selection and breeding course through marker assisted selection. The aim of the current study was to detect polymorphisms of the KiSS1 gene in 137 animals, including Baladi, Zaraibi and Damascus goat breeds by PCR-RFLP, and DNA sequencing and to investigate the association between these variants and reproductive traits. Comparison of the nucleotide sequence indicated the substitution of T with A at position 121 (T121A) in the intron 1 of the KiSS1 gene in all goat breeds. This substitution distorts the restriction site of the XmnI restriction enzyme and consequently two genotypes were detected (TA and TT). The T121A SNP is associated significantly with litter size in Damascus and Zaribi breeds (p=0.025 and 0.001, respectively). The animals with the TT genotype in Damascus and Zaribi breeds had a significantly higher estradiol 17β level than that recorded in TA genotype at estrus phase (p=0.013 and 0.028, respectively) and late-luteal phase (p=0.067 and 0.041, respectively) of the estrus cycle. Furthermore, animals with the TT genotype in Damascus and Zaribi breeds had significant higher progesterone level at mid-luteal (p=0.037 and 0.045, respectively) phase. Meanwhile, there were no significant differences in progesterone level in late-luteal phase between both genotypes in Zaribi breed (p=0.267). The current trial indicated that the prolific TT genotype in both Damascus and Zaribi breeds had superior estradiol 17β level at estrus phase and an eminent progesterone level at both early and mid-luteal phases of the estrous cycle. Copyright © 2017 Elsevier B.V. All rights reserved.

DEALUMINATION OF MORDENITE ZEOLITE AND ITS CATALYTIC ...

African Journals Online (AJOL)

1Department of Chemistry, Faculty of Science, ShahidBahonar University of ..... conversion to branched products, which might be due to the enhanced ... International Zeolite Conference, Butterworth-Heinemann, Boston, MA, 1993, Montreal,.

Low-Temperature Aging of Delta-Ferrite in 316L SS Welds; Changes in Mechanical Properties and Etching Properties

Science.gov (United States)

Abe, Hiroshi; Shimizu, Keita; Watanabe, Yutaka

Thermal aging embrittlement of LWR components made of stainless cast (e.g. CF-8 and CF-8M) is a potential degradation issue, and careful attention has been paid on it. Although welds of austenitic stainless steels (SSs) have γ-δ duplex microstructure, which is similar to that of the stainless cast, examination on thermal aging characteristics of the SS welds is very limited. In order to evaluate thermal aging behavior of weld metal of austenitic stainless steel, the 316L SS weld metal has been prepared and changes in mechanical properties and in etching properties at isothermal aging at 335°C have been investigated. The hardness of the ferrite phase has increased with aging, while the hardness of austenite phase has stayed same. It has been suggested that spinodal decomposition has occurred in δ-ferrite by the 335°C aging. The etching rates of δ-ferrite at immersion test in 5wt% hydrochloric acid solution have been also investigated using an AFM technique. The etching rate of ferrite phase has decreased consistently with the increase in hardness of ferrite phase. It has been thought that this characteristic is also caused by spinodal decomposition of ferrite into chromium-rich (α') and iron-rich (α).

Evaluation at 290 degrees Celsius and 8 MPa of the anticorrosion properties of hydrothermal ceramic nano deposits on preoxidized 304l Ss

Energy Technology Data Exchange (ETDEWEB)

Lopez G, N.; Marin A, M.; Medina A, A. L. [ININ, Carretera Mexico-Toluca s/n, 52750 Ocoyoacac, Estado de Mexico (Mexico); Galicia A, G., E-mail: natali_log@hotmail.com [Universidad Veracruzana, Instituto de Ingenieria, Av. Juan Pablo II s/n, Fracc. Costa Verde, 94294 Boca del Rio, Veracruz (Mexico)

2017-09-15

In the present paper, we present the electrochemical evaluation carried out under reductive conditions (molar ratio H{sub 2}:O{sub 2} >5), at 290 degrees Celsius and 8 MPa, of preoxidized 304l Ss with hydrothermal deposits of nanoparticles of TiO{sub 2} and ZrO{sub 2}. The anticorrosion properties of the deposits were studied by Electrochemical Impedance Spectroscopy (EIS) and Linear Polarization Resistance (LPR). After electrochemical tests, the samples were characterized by X-Ray Diffraction (XRD) and Scanning Electron Microscopy (Sem). It was found by EIS that both, ZrO{sub 2} and TiO{sub 2} produce an increase in the charge transfer resistance (R{sub CT}) of preoxidized 304l Ss. At the same time, by LPR we observed that the studied ceramic nano deposits decrease the corrosion rate (Cr) and corrosion current density (i{sub corr}), however, in most of the cases the Electrochemical Corrosion Potential (Ecp) is slightly higher in the presence of the ceramic nano deposits that the one of preoxidized 304l Ss. After holding time at 290 degrees Celsius, when the impedance (Z) increases, the capacitance increases also, which is attributed to a surface increase due to the roughness of the oxide formed at this temperature under a reductive environment. The observed electrochemical behavior has a dependence on the homogeneity and thickness of the deposits, as has been corroborated by Sem and XRD. The best electrochemical results are obtained when two conditions are fulfilled: The bigger the effective coated area and the lower the deposit thickness. (Author)

75 FR 51531 - Proposed Collection; Comment Request for Forms 941, 941-PR, 941-SS, 941-X, 941-X(PR), Schedule B...

Science.gov (United States)

2010-08-20

... 941, 941-PR, 941- SS, 941-X, 941-X(PR), Schedule B (Form 941), Schedule R (Form 941) and Schedule B (Form 941-PR) AGENCY: Internal Revenue Service (IRS), Treasury. ACTION: Notice and request for comments... comments concerning Forms 941 (Employer's Quarterly Federal Tax Return), 941-PR (Planilla Para La...

Soovitan filmi ja raamatut / Kaja Peterson

Index Scriptorium Estoniae

Peterson, Kaja

2012-01-01

Tutvustus: Marianne Kõrveri ja Kaie Kotovi keskkonnafilmist "Inimese mõõt". Testfilm, 2010/2011 ; Saunders, Doug. Arrival City : how the largest migration in history is reshaping our world. London : William Heinemann, 2010

A Study on the Fatigue-Fractured Surface of Normalized SS41 Steel and M.E.F. Dual Phase Steel by an X-ray Diffraction Technique

International Nuclear Information System (INIS)

Oh, Sae Wook; Park, Young Chul; Park, Soo Young; Kim, Deug Jin; Hue, Sun Chul

1996-01-01

This study verified the relationship between fracture mechanics parameters and X-ray parameters for normalized SS41 steel with homogeneous crystal structure and M.E.F. dual phase steel(martensite encapsulated islands of ferrite). The fatigue crack propagation test were carried out and X-ray diffraction technique was applied to fatigue fractured surface. The change in X-ray parameters(residual stress, half-value breadth) according to the depth of fatigue fractured surface were investigated. The depth of maximum plastic zone, W y , were determined on the basis of the distribution of the half-value breadth for normalized SS41 steel and that of the residual stress for M.E.F. dual phase steel. K max could be estimated by the measurement of W y

WAJM 28(3)FINAL6cd.pmd

African Journals Online (AJOL)

daddy

cellular immunity in normal pregnancy and an enhanced cell mediated ... Hospital, Lagos, †Clinical Chemistry Division, John Sealy Hospital, University of Texas Medical Branch, Glaveston, Texas. ..... 2nd edition, Heinemann, Ibadan. 2001 ...

Fluidized pellet bed bioreactor system for SS, COD, nitrogen and phosphorus; Ryudoso zoryu bio reactor system ni yoru SS, COD, chisso oyobi rin jokyo

Energy Technology Data Exchange (ETDEWEB)

Shimizu, T.; Tanbo, N.; Kudo, K. [Hokkaido University, Sapporo (Japan). Faculty of Engineering; Hamaguchi, T.; Nakabayashi, A. [Tsukishima Kikai Co. Ltd., Tokyo (Japan)

1995-02-10

An examination was carried out on a treatment system which was constituted of an aerobic fluidized pellet bed (AFRB) bioreactor and the two steps comprising a contact aeration process and a sand filtration process for sewage/drainage treatment. The following data were obtained from the experiment by a pilot plant installed in a sewage plant. The removal ratios were the same as or above 95% in SS, 95% in total phosphorus, 85% in COD and 80% in total nitrogen. The highly dense sludge pellets formed in the AFPB bioreactor in summer contained aerobic filamentous bacteria that were capable of multiplying. The sludge retention time of this bioreactor was 2.2 to 8.1 days which were sufficient for the multiplication of denitrifiers, whose number and activity were also satisfactory for dinitrification. Further, other microorganisms existed in the bioreactor such as sulfate reducers and methanation bacteria. The role of a contact aeration tank was the oxidized decomposition and nitration of soluble BOD, and the biofilm had niterite and nitrate bacteria adhered to it. Assuming the retention time of the tank was two hours, the nitration ratio was 90% or more at the water temperature of 15{degree}C or higher. 29 refs., 12 figs., 2 tabs.

Influence of Mn-Co Spinel Coating on Oxidation Behavior of Ferritic SS Alloys for SOFC Interconnect Applications

DEFF Research Database (Denmark)

Venkatachalam, Vinothini; Molin, Sebastian; Kiebach, Wolff-Ragnar

2014-01-01

Chromia forming ferritic stainless steels (SS) are being considered for intermediate temperature solid oxide fuel cell interconnect applications. However, protective coatings are in general needed to avoid chromium volatilization and poisoning of cathodes from chromium species. Mn-Co spinel is one...... of the promising candidates to prevent chromium outward diffusion, improve oxidation resistance and ensure high electrical conductivity over the lifetime of interconnects. In the present study, uniform and well adherent Mn-Co spinel coatings were produced on Crofer 22APU using electrophoretic deposition (EPD...

Browse Title Index

African Journals Online (AJOL)

Vol 13, No 34 (2014), Use of spent compost in the cultivation of Agaricus blazei ... assafoetida essential oil as adult carob moth repellent in Qom pomegranate ... in frozen chicken and chicken products collected from Riyadh, Saudi Arabia ...

[Myocardiopathy diagnosed in utero in a mother with SS-A antibodies treated with plasmapheresis].

Science.gov (United States)

Arroyave, C M; Puente Ledezma, F; Montiel Amoroso, G; Martínez García, A C

1995-03-01

We report a 36 years old patient with Sjogren's syndrome, who during her second pregnancy, the product developed a miocardiopathy with complete heart block that was diagnosed in utero at 26 weeks of pregnancy. Simultaneously, laboratory data reported a SS-A/Ro titer of 1:50,000 with positive antiphospholipids antibodies. Patient was subjected three times to plasmapheresis with three blood volume exchange each time. During the procedures, we had monitor the product and no hemodinamic changes were observed. Unfortunately, 25 days later the patient reported absence of fetal movement and by ecosonography and Doppler was not observed fetal movement or cardiac function. This pregnancy ends in cesarea. The patient is in perfect clinical conditions under control using prednisone and methotrexate.

Dr Flavia Schlegel Assistant Director-General for Natural Sciences UNESCO

CERN Multimedia

Bennett, Sophia Elizabeth

2016-01-01

27 January 2016 - UNESCO Assistant Director-General for Natural Sciences UNESCO F. Schlegel visiting the ATLAS experimental cavern with Collaboration Deputy Spokesperson B. Heinemann. M. Bona, Relations with International Organisations, accompanies the delegation throughout.

Structural and chemical analysis of silica-doped β-TCP ceramic coatings on surgical grade 316L SS for possible biomedical application

Directory of Open Access Journals (Sweden)

Karuppasamy Prem Ananth

2015-09-01

Full Text Available We have developed a novel approach to introduce silica-doped β-tricalcium phosphate (Si-β-TCP on 316L SS substrates for enhanced biological properties. Doping of β-TCP with silica loadings ranging from 0 to 8 mol% was carried out using chemical precipitation method. Si-β-TCP powder was sintered at 800 °C followed by coating it on 316L SS substrate using electrophoretic deposition. The coated and uncoated samples were investigated by various characterization techniques such as X-ray diffraction (XRD, Fourier transform infrared spectroscopy (FTIR, field emission scanning electron microscopy (FESEM and X-ray fluorescence spectroscopy (XRF. Biomineralization ability of the coatings was evaluated by immersing in simulated body fluid (SBF solution for different number of days such as 7, 14, 21 and 28 days. The results obtained in our study have shown that the apatite formation ability was high for the 8 mol% of Si-β-TCP. This will promote better biomineralization ability compared to the other coatings.

Larvicidal effect of disinfectant soap on Anopheles gambiae s.s (Diptera: Culicidae) in laboratory and semifield environs.

Science.gov (United States)

Mdoe, France P; Nkwengulila, Gamba; Chobu, Mariam; Lyaruu, Lucile; Gyunda, Israel L; Mbepera, Saada; Xue, Rui-De; Kweka, Eliningaya J

2014-05-03

Mosquito larval control using chemicals and biological agents is of paramount importance in vector population and disease incidence reduction. A commercial synthetic disinfectant soap was evaluated against larvae of Anopheles gambiae s.s. in both laboratory and semi field conditions. Five concentrations of commercial synthetic disinfectant soap (0.0001, 0.001, 0.01, 0.1 and 1%) were prepared and evaluated against third instar larvae in laboratory and semi field environments. Mortality was scored at 12, 24, 48, and 72 hrs. Each dosage had 6 replicates, having twenty 3rd instar larvae of An.gambiae s.s. In the laboratory phase, all dosages had significantly higher larval mortalities than in controls, while in semi field conditions, the dosages of 0.0001, 0.001 and 0.01% had lower mortalities than laboratory trials. In the comparison between semi field and laboratory trials, only 0.1 and 1% dosage had significant difference with more mortality in semifield conditions. Proportions of larvae that died during mortality monitoring intervals in laboratory and semi field had significant differences only at 12 hrs and 72 hrs. The findings of this study have demonstrated that the mortality of larvae caused by commercial synthetic disinfectant soap is worth further studies in open water bodies. More studies are necessary to find out the effect of sunlight on the chemistry of the synthetic disinfectant and other variables in small scale full field trials.

Combined slurry and cavitation erosion resistance of surface modified SS410 stainless steel

Science.gov (United States)

Amarendra, H. J.; Pratap, M. S.; Karthik, S.; Punitha Kumara, M. S.; Rajath, H. C.; Ranjith, H.; Shubhatunga, S. V.

2018-03-01

Slurry erosion and combined slurry and cavitation erosion resistance of thermal spray coatings are studied and compared with the as-received martensitic stainless steel material. 70Ni-Cr coatings are deposited on SS 410 material through plasma thermal spray process. The synergy effect of the combined slurry and cavitation erosion resistance of plasma thermal spray coatings were investigated in a slurry pot tester in the presence of bluff bodies known as Cavitation Inducers. Results showed the combined slurry and cavitation erosion resistance of martensitic stainless steel - 410 can be improved by plasma thermal spray coating. It is observed that the plasma spray coated specimens are better erosion resistant than the as- received material, subjected to erosion test under similar conditions. As-received and the surface modified steels are mechanically characterized for its hardness, bending. Morphological studies are conducted through scanning electron microscope.

Effect of current and travel speed variation of TIG welding on microstructure and hardness of stainless steel SS 316L

Science.gov (United States)

Jatimurti, Wikan; Abdillah, Fakhri Aulia; Kurniawan, Budi Agung; Rochiem, Rochman

2018-04-01

One of the stainless steel types that widely used in industry is SS 316L, which is austenitic stainless steel. One of the welding methods to join stainless steel is Tungsten Inert Gas (TIG), which can affect its morphology, microstructure, strength, hardness, and even lead to cracks in the weld area due to the given heat input. This research has a purpose of analyzing the relationship between microstructure and hardness value of SS 316L stainless steel after TIG welding with the variation of current and travel speed. The macro observation shows a distinct difference in the weld metal and base metal area, and the weld form is not symmetrical. The metallographic test shows the phases that formed in the specimen are austenite and ferrite, which scattered in three welding areas. The hardness test showed that the highest hardness value found in the variation of travel speed 12 cm/min with current 100 A. Welding process and variation were given do not cause any defects in the microstructure, such as carbide precipitation and sigma phase, means that it does not affect the hardness and corrosion resistance of all welded specimen.

Surface plasmon resonance imaging reveals multiple binding modes of Agrobacterium transformation mediator VirE2 to ssDNA.

Science.gov (United States)

Kim, Sanghyun; Zbaida, David; Elbaum, Michael; Leh, Hervé; Nogues, Claude; Buckle, Malcolm

2015-07-27

VirE2 is the major secreted protein of Agrobacterium tumefaciens in its genetic transformation of plant hosts. It is co-expressed with a small acidic chaperone VirE1, which prevents VirE2 oligomerization. After secretion into the host cell, VirE2 serves functions similar to a viral capsid in protecting the single-stranded transferred DNA en route to the nucleus. Binding of VirE2 to ssDNA is strongly cooperative and depends moreover on protein-protein interactions. In order to isolate the protein-DNA interactions, imaging surface plasmon resonance (SPRi) studies were conducted using surface-immobilized DNA substrates of length comparable to the protein-binding footprint. Binding curves revealed an important influence of substrate rigidity with a notable preference for poly-T sequences and absence of binding to both poly-A and double-stranded DNA fragments. Dissociation at high salt concentration confirmed the electrostatic nature of the interaction. VirE1-VirE2 heterodimers also bound to ssDNA, though by a different mechanism that was insensitive to high salt. Neither VirE2 nor VirE1-VirE2 followed the Langmuir isotherm expected for reversible monomeric binding. The differences reflect the cooperative self-interactions of VirE2 that are suppressed by VirE1. © The Author(s) 2015. Published by Oxford University Press on behalf of Nucleic Acids Research.

Assessment of water-soluble thiourea-formaldehyde (WTF) resin for stabilization/solidification (S/S) of heavy metal contaminated soils.

Science.gov (United States)

Liu, She-Jiang; Jiang, Jia-Yu; Wang, Shen; Guo, Yu-Peng; Ding, Hui

2018-03-15

Stabilization/Solidification (S/S) can be regarded as necessary for remediation of heavy metal contaminated soil. There is, however, solid agent is not very convenient to use. Water-soluble thiourea-formaldehyde (WTF) is a novel chelating agent, which has more practical applications. The process of WTF resin for S/S process of heavy metal contaminated soils was studied. Laboratory-prepared slurries, made of field soils spiked with Cd 2+ and Cr 6+ were treated with WTF resin. The toxicity characteristic leaching procedure (TCLP) showed that with 2 wt% WTF, in the neutral condition of soil after treatment for 7 d, the leaching concentrations of Cd 2+ and Cr 6+ in contaminated soil were decreased by 80.3% and 92.6% respectively. Moreover, Tessier sequence extraction procedure showed WTF resin reduced the leaching concentration by transforming heavy metal from exchange form to organic form. The structure of WTF is obtained according to elemental analysis result and reaction mechanism. Through analysis of the infrared spectrogram of WTF and WTF heavy mental chelating precipitation, WTF can form stable chelate with heavy mental through coordination. The significant groups are hydroxyl, nitrogen and sulphur function groups in WTF mainly. Toxicology test revealed that the WTF resin is nontoxic to microorganism in the soils. Copyright © 2017 Elsevier B.V. All rights reserved.

01 WestPavlov 03 WEB.pmd

African Journals Online (AJOL)

Owner

works, a definition of a metropolis always ahead of itself, renders the book of the city publishable ... Significantly, Nuttall and Mbembe reference an “unpublished draft” (Nuttall and. Mbembe, eds ...... Harvard UP/London: Heinemann,. 1983.

Review: Kate Meagher, Identity Economics: Social Networks and the Informal Economy in Nigeria (2010)

OpenAIRE

Axel Harneit-Sievers

2010-01-01

Review of the monograph: Kate Meagher (2010), Identity Economics: Social Networks and the Informal Economy in Nigeria , Woodbridge, Suffolk: James Currey / Ibadan: Heinemann Educational Books (Nigeria), ISBN 978-1-84701-016-2, xv+208 pages.

Application of a qPCR assay in the investigation of susceptibility to malaria infection of the M and S molecular forms of An. gambiae s.s. in Cameroon.

Directory of Open Access Journals (Sweden)

Anne Boissière

Full Text Available Plasmodium falciparum is the causative agent of malaria, a disease that kills almost one million persons each year, mainly in sub-Saharan Africa. P. falciparum is transmitted to the human host by the bite of an Anopheles female mosquito, and Anopheles gambiae sensus stricto is the most tremendous malaria vector in Africa, widespread throughout the afro-tropical belt. An. gambiae s.s. is subdivided into two distinct molecular forms, namely M and S forms. The two molecular forms are morphologically identical but they are distinct genetically, and differ by their distribution and their ecological preferences. The epidemiological importance of the two molecular forms in malaria transmission has been poorly investigated so far and gave distinct results in different areas. We have developed a real-time quantitative PCR (qPCR assay, and used it to detect P. falciparum at the oocyst stage in wild An. gambiae s.s. mosquitoes experimentally infected with natural isolates of parasites. Mosquitoes were collected at immature stages in sympatric and allopatric breeding sites and further infected at the adult stage. We next measured the infection prevalence and intensity in female mosquitoes using the qPCR assay and correlated the infection success with the mosquito molecular forms. Our results revealed different prevalence of infection between the M and S molecular forms of An. gambiae s.s. in Cameroon, for both sympatric and allopatric populations of mosquitoes. However, no difference in the infection intensity was observed. Thus, the distribution of the molecular forms of An. gambiae s.s. may impact on the malaria epidemiology, and it will be important to monitor the efficiency of malaria control interventions on the two M and S forms.

Engineering Evaluation and Assessment (EE and A) Report for the Symbolic and Sub-symbolic Robotics Intelligence Control System (SS-RICS)

Science.gov (United States)

2018-04-01

ARL-TR-8352 ● APR 2018 US Army Research Laboratory Engineering Evaluation and Assessment (EE&A) Report for the Symbolic and Sub...APR 2018 US Army Research Laboratory Engineering Evaluation and Assessment (EE&A) Report for the Symbolic and Sub-symbolic Robotics...Intelligence Control System (SS-RICS) by Troy Dale Kelley and Eric Avery Human Research and Engineering Directorate, ARL Sean McGhee STG Inc

Fusion welding studies using laser on Ti-SS dissimilar combination

Science.gov (United States)

Shanmugarajan, B.; Padmanabham, G.

2012-11-01

Laser welding investigations were carried out on dissimilar Ti-SS combination. The study is aimed to improve the weld strength and ductility by minimizing harmful intermetallics and taking advantage of high cooling rates in laser welding. Results of continuous wave 3.5 kW CO2 laser welding of totally dissimilar combination of Titanium and stainless steel (304) have been discussed. Bead on plate welding experiments were conducted to identify the laser welding parameters using depth of penetration as criteria. The welding of dissimilar combination has been attempted both autogenously and with interlayers such as Vanadium (V) and Tantalum (Ta) in the form of laser cladding as well as strip. Autogenous welds were carried out by varying the laser power, welding speed and position of the laser beam with respect to the joint centre. The resultant welds are characterized by macrostructure analysis, SEM/EDAX and XRD and as welded tensile test in UTM. The autogenous welds have exhibited extensive cracking even when welded at high speeds or by manipulating the beam position with respect to the joint. Similarly Vandaium as interlayer could not achieve crack free joint. A joint with 40 MPa strength could be made with Ta as interlayer. Results and analysis of these variants of laser welded joints are reported and discussed.

Auto-anticorpos na Esclerose Sistêmica (ES Autoantibody in the Systemic Sclerosis (SS

Directory of Open Access Journals (Sweden)

Luís Eduardo Coelho Andrade

2004-06-01

Full Text Available A esclerose sistêmica (ES é uma enfermidade inflamatória crônica idiopática, cujo principal indício de vínculo com a auto-imunidade é a presença de auto-anticorpos na maior parte dos pacientes. Pelo teste de imunofluorescência indireta em células HEp-2, observa-se reatividade predominantemente contra o nucléolo e o núcleo, sendo que títulos de anticorpos antinucléolo acima de 1/640 são fortemente sugestivos de ES. Alguns desses auto-anticorpos apresentam alta especificidade para a ES, sendo considerados marcadores diagnósticos dessa enfermidade. São exemplos os anticorpos anti-Scl-70, antifibrilarina e anti-RNA polimerase I. Outros apresentam interessantes associações com manifestações específicas da ES, como os anticorpos anticentrômero, associados às formas limitadas, os anticorpos anti-RNA polimerase III, associados ao extenso comprometimento da pele, e os anticorpos anti-To/Th, associados às formas limitadas com propensão ao desenvolvimento de hipertensão pulmonar. Algumas dessas associações estão bem estabelecidas em diversos estudos, de diferentes grupos de pesquisadores. Outras, entretanto, devem ser vistas com cautela, pois a exigüidade de casos disponíveis para estudo pode ensejar conclusões preliminares e não acuradas. A detecção de anticorpos anticentrômero por técnica de IFI e anti-Scl-70 por imunodifusão dupla ou por ELISA está disponível nos principais laboratórios clínicos capacitados na área de auto-imunidade. O mesmo não se aplica para a maior parte dos demais auto-anticorpos associados à ES. Espera-se que, com a implementação rotineira de técnicas para detecção desses outros auto-anticorpos, o real significado clínico dos mesmos venha a ser melhor conhecido.Systemic Sclerosis (SS is a chronic idiopathic inflammatory disease whose main hint towards autoimmunity is given by the presence of high serum levels of autoantibodies in the majority of the patients. In indirect

short communication mathematical modelling for magnetite

African Journals Online (AJOL)

Preferred Customer

The present research focuses to develop mathematical model for the ..... Staler, M.J. The Principle of Ion Exchange Technology, Butterworth-Heinemann: Boston; ... Don, W.G. Perry's Chemical Engineering Hand Book, 7th ed., McGraw-Hill:.

Partir et revenir au village Mobilité non permanente des jeunes ruraux dans la région du Saïss (Maroc)

NARCIS (Netherlands)

Ftouhi, Hind; Kadiri, Zakaria; Abdellaoui, El Hassane; Bossenbroek, Lisa

2015-01-01

This article analyzes the role of the non-permanent mobility of rural youth in the territorial development in the agricultural plain of the Saïss in Morocco. This mobility refers to the frequent moves by young people for relatively short periods, to study and work, with frequent returns to the

Repellent and deterrent effects of SS220, Picaridin, and Deet suppress human blood feeding by Aedes aegypti, Anopheles stephensi, and Phlebotomus papatasi.

Science.gov (United States)

Klun, Jerome A; Khrimian, Ashot; Debboun, Mustapha

2006-01-01

A series of behavioral tests with Aedes aegypti (L.), Anopheles stephensi Liston, mosquitoes, and the sand fly Phlebotomus papatasi Scopoli in the presence of Deet, SS220, and Picaridin topically applied to the skin of human volunteers showed that the insects were deterred from feeding on and repelled from surfaces emanating the compounds. When offered a 12- or 24-cm2 area of skin, one-half treated with compound and one-half untreated, the insects fed almost exclusively on untreated skin. The sand flies and mosquitoes did not at any time physically contact chemically treated surfaces. When treated and untreated skin areas were covered with cloth, insects contacted, landed, and bit only through cloth covering untreated skin. These observations provided evidence that the compounds deterred feeding and repelled insects from treated surfaces primarily as a result of olfactory sensing. When cloth, one-half untreated and one-half treated with chemical, was placed over untreated skin, insects only touched and specifically bit through the untreated cloth. This showed that the activity of the chemicals does not involve a chemical x skin interaction. In the presence of any of the three chemicals, no matter how they were presented to the insects, overall population biting activity was reduced by about one-half relative to controls. This reduction showed a true repellent effect for the compounds. Results clearly showed that Deet, SS220, and Picaridin exert repellent and deterrent effects upon the behavior of mosquitoes and sand flies. Heretofore, the combined behavioral effects of these compounds upon mosquito and sand fly behavior were unknown. Moreover, protection afforded by Deet, SS220, and Picaridin against the feeding of these three disease vectors on humans is mechanistically a consequence of the two chemical effects.

manhood patronage as transmutation in the novels of akachi ...

African Journals Online (AJOL)

DGS-FUTO

2018-06-01

Jun 1, 2018 ... Certain myths are adopted in Things Fall Apart, with the intention to caricature ..... provision of shelter for Richard and also Kainene‟s suite at her father‟s Zobis Hotel where .... Ibadan: Heinemann Educational Books,.

1 September 2015 - CODEL Lucas, Members of the Committee on Science, Space and Technology, United States of America

CERN Multimedia

Brice, Maximilien

2015-01-01

visiting the ATLAS Visitors Centre and the ATLAS cavern with B. Heinemann, ATLAS Collaboration Deputy Spokesperson, Lawrence Berkeley National Laboratory and University of California, Berkeley and signing the CERN Guest Book with the Director-General R. Heuer.

PRP19 transforms into a sensor of RPA-ssDNA after DNA damage and drives ATR activation via a ubiquitin-mediated circuitry.

Science.gov (United States)

Maréchal, Alexandre; Li, Ju-Mei; Ji, Xiao Ye; Wu, Ching-Shyi; Yazinski, Stephanie A; Nguyen, Hai Dang; Liu, Shizhou; Jiménez, Amanda E; Jin, Jianping; Zou, Lee

2014-01-23

PRP19 is a ubiquitin ligase involved in pre-mRNA splicing and the DNA damage response (DDR). Although the role for PRP19 in splicing is well characterized, its role in the DDR remains elusive. Through a proteomic screen for proteins that interact with RPA-coated single-stranded DNA (RPA-ssDNA), we identified PRP19 as a sensor of DNA damage. PRP19 directly binds RPA and localizes to DNA damage sites via RPA, promoting RPA ubiquitylation in a DNA-damage-induced manner. PRP19 facilitates the accumulation of ATRIP, the regulatory partner of the ataxia telangiectasia mutated and Rad3-related (ATR) kinase, at DNA damage sites. Depletion of PRP19 compromised the phosphorylation of ATR substrates, recovery of stalled replication forks, and progression of replication forks on damaged DNA. Importantly, PRP19 mutants that cannot bind RPA or function as an E3 ligase failed to support the ATR response, revealing that PRP19 drives ATR activation by acting as an RPA-ssDNA-sensing ubiquitin ligase during the DDR. Copyright © 2014 Elsevier Inc. All rights reserved.

Electrical conductivity and physical changes of functionalized carbon nanotube/graphite/stainless steel (SS316L/polypropylene composites immersed in an acidic solution

Directory of Open Access Journals (Sweden)

Hendra Suherman

2018-02-01

Full Text Available Chemical functionalization of carbon nanotubes (CNTs and graphite (G was performed to improve their adhesion to a metal powder (SS316L and a polypropylene (PP matrix. A conductive pathway was thus formed to utilize the high electrical conductivity of the SS316L for conductive polymer composites (CPCs applications. The rheological properties of the feedstock mixture were measured, and samples were formed by injection molding. Fourier transform infrared spectroscopy indicated the existence of functional groups generated by chemical wet oxidation. Only feedstock mixtures containing more than 28 vol% of the PP composite showed a shear rate (102 s -1 to 105 s -1 , shear viscosity (10 Pa·s to 103 Pa·s, and flow behavior index (n<1 suitable for injection molding. The functionalized composites exhibited improved electrical and mechanical properties over the as-produced composites. The functionalized composites containing 28 vol% PP obtained an electrical conductivity of 6 S/m, compared to 0.5 S/m for the as-produced composites.

BÚSQUEDA DE LA MUTACIÓN Phe-809 EN EL GEN DEL SUSTRATO DEL RECEPTOR DE INSULINA -1 (IRS-1 EN RATAS DIABÉTICAS eSS

Directory of Open Access Journals (Sweden)

Stella Maris Daniele

2010-01-01

Full Text Available El gen del sustrato del receptor de insulina IRS-1 juega un rol clave en la transducción de señales de insulina en músculo esquelético. Diversos polimorfismos del gen IRS-1 han sido reportados en estados de insulinorresistencia como obesidad y diabetes tipo 2.Las ratas eSS desarrollan espontáneamente diabetes tipo 2 magra, con insulinoresistencia caracterizada por hiperglucemia e hiperinsulinemia. Durante el segundo año existe disminución progresiva de la insulinemia y agravamiento del síndrome. Nuestro propósito fue buscar la mutación Phe-809 en el gen IRS-1, que implica sustitución del aminoácido Ser por Phe en el codon 809; dicho sitio se halla conservado en la rata. Esta variante fue identificada en pacientes diabéticos tipo 2. El gen del IRS-1 consta de un solo exón.Se analizó un fragmento de 281pb del gen IRS-1, que posee 90% de homología con el mismo fragmento en humanos. Se purificó ADN de leucocitos de un pool de sangre de ratas machos eSS y controles Wistar. Se cuantificó y amplificó por PCR utilizando un par de primers para amplificar ese mismo fragmento en el gen IRS-1 en humanos. Se usó sangre humana como control de amplificación. Se empleó el método de Sanger en la secuenciación del fragmento obtenido por PCR, previa extracción del mismo de un gel de agarosa al 2%. Se observó que la rata eSS y las controles mantuvieron la lectura esperada para ese fragmento, no encontrándose la mutación investigada. Se descarta la mutación Phe-809 como origen de la insulinoresistencia de la rata eSS.

Spa47 is an oligomerization-activated type three secretion system (T3SS) ATPase from Shigella flexneri.

Science.gov (United States)

Burgess, Jamie L; Jones, Heather B; Kumar, Prashant; Toth, Ronald T; Middaugh, C Russell; Antony, Edwin; Dickenson, Nicholas E

2016-05-01

Gram-negative pathogens often use conserved type three secretion systems (T3SS) for virulence. The Shigella type three secretion apparatus (T3SA) penetrates the host cell membrane and provides a unidirectional conduit for injection of effectors into host cells. The protein Spa47 localizes to the base of the apparatus and is speculated to be an ATPase that provides the energy for T3SA formation and secretion. Here, we developed an expression and purification protocol, producing active Spa47 and providing the first direct evidence that Spa47 is a bona fide ATPase. Additionally, size exclusion chromatography and analytical ultracentrifugation identified multiple oligomeric species of Spa47 with the largest greater than 8 fold more active for ATP hydrolysis than the monomer. An ATPase inactive Spa47 point mutant was then engineered by targeting a conserved Lysine within the predicted Walker A motif of Spa47. Interestingly, the mutant maintained a similar oligomerization pattern as active Spa47, but was unable to restore invasion phenotype when used to complement a spa47 null S. flexneri strain. Together, these results identify Spa47 as a Shigella T3SS ATPase and suggest that its activity is linked to oligomerization, perhaps as a regulatory mechanism as seen in some related pathogens. Additionally, Spa47 catalyzed ATP hydrolysis appears to be essential for host cell invasion, providing a strong platform for additional studies dissecting its role in virulence and providing an attractive target for anti-infective agents. © 2016 The Protein Society.

Evaluation of the antigenotoxic effects of the royal sun mushroom, agaricus brasiliensis (Higher basidiomycetes) in human lymphocytes treated with thymol in the comet assay

NARCIS (Netherlands)

Radaković, Milena; Stevanović, Jevrosima; Soković, Marina; Radović, Dejan; Griensven, Van Leo J.L.D.; Stanimirović, Zoran

2015-01-01

The aim of this investigation was to evaluate the possible protective activity of Agaricus brasiliensis (=A. blazei sensu Murrill) ethanol extract against thymol-induced DNA damage in human lymphocytes. Before we studied the possible interaction of thymol and A. brasiliensis extract, each

Effect Of Irradiation Temperature and Dose On Mechanical Properties And Fracture Characteristics Of Cu//SS Joints For ITER

International Nuclear Information System (INIS)

Fabritsiev, S.A.; Pokrovsky, A.S.; Peacock, A.; Roedig, M.; Linke, J.; Gervash, A.; Barabash, V.

2007-01-01

Full text of publication follows: By now, a number of technologies have been proposed for the production of Cu//SS joints for ITER, such as brazing, friction welding, HIP and cast-copper-to-steel (CC). The two last-mentioned technologies ensure sufficiently high mechanical properties and a high joint quality, when unirradiated. The data, however, on mechanical characteristics of irradiated of Cu//SS HIP joints are limited. In this paper, the authors present the results of investigations into the mechanical characteristics after irradiation of GlidCopAl25/316L(N) and Cu-Cr-Zr/316L(N)-type joints produced by the HIP and CC technologies. Specimens of the joints were irradiated in the RBT-6 reactor in the dose range of 10 -3 - 10 -1 dpa at T irr = 200 deg. C and 300 deg. C. The tensile stress-strain curves for irradiated and unirradiated joint specimens show deformation processes occurring in both the Cu and SS parts of the specimens. Irradiation at T irr = 200 deg. C causes strengthening of the joints specimens (by about 100 MPa at the maximum dose). The uniform elongation drops from 8% in the initial state to 2-3 %. But the total elongation remains at a relatively high level of ∼ 7%. Irradiation at T irr = 300 deg. C causes a slight strengthening of the joints specimens (∼30 MPa). The uniform elongation remains unchanged at ∼ 7%. The total elongation also maintains a relatively high level of ∼9-13%. SEM investigations revealed that fracture occurs only in the copper part of the irradiated specimens, and ductile trans-crystalline fracture predominates in the joints. 3D finite element analysis of the tensile test indicates that the concentration of stresses and deformations in the copper layer adjacent to the joint line is responsible for this typical failure of the irradiated joints specimens. Comparison of the behavior of the joints irradiated at T irr = 200 deg. C and 300 deg. C indicate an increased embrittlement at lower irradiation temperatures. At a

Preventing Pressure Sores

Medline Plus

Full Text Available ... Cord Injury Allen Heinemann, PhD How Peer Counseling Works Julie Gassaway, MS, RN Pediatric Injuries Pediatric Spinal ... does not provide medical advice, recommend or endorse health care products or services, or control the information ...

Sex and Fertility After SCI

Medline Plus

Full Text Available ... Cord Injury Allen Heinemann, PhD How Peer Counseling Works Julie Gassaway, MS, RN Pediatric Injuries Pediatric Spinal ... does not provide medical advice, recommend or endorse health care products or services, or control the information ...

Spinal Cord Injury 101

Medline Plus

Full Text Available ... Cord Injury Allen Heinemann, PhD How Peer Counseling Works Julie Gassaway, MS, RN Pediatric Injuries Pediatric Spinal ... injury? play_arrow How does the spinal cord work? play_arrow Why is the level of a ...

Sex and Fertility After SCI

Medline Plus

Full Text Available ... Spinal Cord Injury Guy W. Fried, MD Substance Abuse and Spinal Cord Injury Allen Heinemann, PhD How ... spinal cord injury? play_arrow Can men and women still have sex after a spinal cord injury? ...

Sex and Fertility After SCI

Medline Plus

Full Text Available ... Cord Injury Allen Heinemann, PhD How Peer Counseling Works Julie Gassaway, MS, RN Pediatric Injuries Pediatric Spinal ... play_arrow How is the delivery of a child affected by the mother's spinal cord injury? play_ ...

Basics of SCI Rehabilitation

Medline Plus

Full Text Available ... Spinal Cord Injury Guy W. Fried, MD Substance Abuse and Spinal Cord Injury Allen Heinemann, PhD How ... the most important thing for families to know right away? play_arrow What are the main concerns ...

Spinal Cord Injury 101

Medline Plus

Full Text Available ... Spinal Cord Injury Guy W. Fried, MD Substance Abuse and Spinal Cord Injury Allen Heinemann, PhD How ... arrow Why are high-dose steroids often used right after an injury? play_arrow What is meant ...

Sex and Fertility After SCI

Medline Plus

Full Text Available ... Cord Injury Allen Heinemann, PhD How Peer Counseling Works Julie Gassaway, MS, RN Pediatric Injuries Pediatric Spinal ... arrow How does caregiving for your partner often affect romantic relationships? play_arrow What are the common ...

Sex and Fertility After SCI

Medline Plus

Full Text Available ... Spinal Cord Injury Guy W. Fried, MD Substance Abuse and Spinal Cord Injury Allen Heinemann, PhD How ... play_arrow How is the delivery of a child affected by the mother's spinal cord injury? play_ ...

Estimating crustal thickness using SsPmp in regions covered by low-velocity sediments: Imaging the Moho beneath the Southeastern Suture of the Appalachian Margin Experiment (SESAME) array, SE Atlantic Coastal Plain

Science.gov (United States)

Parker, E. Horry, Jr.; Hawman, Robert B.; Fischer, Karen M.; Wagner, Lara S.

2016-09-01

Deconvolved waveforms for two earthquakes (Mw: 6.0 and 5.8) show clear postcritical SsPmp arrivals for broadband stations deployed across the coastal plain of Georgia, allowing mapping of crustal thickness in spite of strong reverberations generated by low-velocity sediments. Precritical SsPmp arrivals are also identified. For a basement in which velocity increases linearly with depth, a bootstrapped grid search suggests an average basement velocity of 6.5 ± 0.1 km/s and basement thickness of 29.8 ± 2.0 km. Corresponding normal-incidence Moho two-way times (including sediments) are 10.6 ± 0.6 s, consistent with times for events interpreted as Moho reflections on coincident active-source reflection profiles. Modeling of an underplated mafic layer (Vp = 7.2-7.4 km/s) using travel time constraints from SsPmp data and vertical-incidence Moho reflection times yields a total basement thickness of 30-35 km and average basement velocity of 6.35-6.65 km/s for an underplate thickness of 0-15 km.

TEM characterization of corrosion products formed on a SS-15ZR alloy

International Nuclear Information System (INIS)

Luo, J. S.; Abraham, D. P.

2000-01-01

The corrosion products formed on a stainless steel-15Zr (SS-15Zr) alloy have been characterized by transmission electron microscopy (TEM) and energy dispersive x-ray spectroscopy (EDS). Examination of alloy particles that were immersed in 90 C deionized water for two years revealed that different corrosion products were formed on the stainless steel and intermetallic phases. Two corrosion products were identified on an austenite particle: trevorite (NiFe 2 O 4 ) in the layer close to the metal and maghemite (Fe 2 O 3 ) in the outer layer. The corrosion layer formed on the intermetallic was uniform, adherent, and amorphous. The EDS analysis indicated that the layer was enriched in zirconium when compared with the intermetallic composition. High-resolution TEM images of the intermetallic-corrosion layer interface show an interlocking metal-oxide interface which may explain the relatively strong adherence of the corrosion layer to the intermetallic surface. These results will be used to evaluate corrosion mechanisms and predict long-term corrosion behavior of the alloy waste form

Evaluation of two counterflow traps for testing behaviour-mediating compounds for the malaria vector Anopheles gambiae s.s. under semi-field conditions in Tanzania

NARCIS (Netherlands)

Schmied, W.H.; Takken, W.; Killeen, G.F.; Knols, B.G.J.; Smallegange, R.C.

2008-01-01

Background Evaluation of mosquito responses towards different trap-bait combinations in field trials is a time-consuming process that can be shortened by experiments in contained semi-field systems. Possible use of the BG Sentinel (BGS) trap to sample Anopheles gambiae s.s. was evaluated. The

Basics of SCI Rehabilitation

Medline Plus

Full Text Available ... Cord Injury Allen Heinemann, PhD How Peer Counseling Works Julie Gassaway, MS, RN Pediatric Injuries Pediatric Spinal ... How soon should people return to school or work after a spinal cord injury? play_arrow What’s ...

Tytöt pelitoiminnallisessa nuorisotyössä : Helsingin nuorisoasiainkeskuksen Pelitalon tyttökävijöiden kokemuksia toiminnasta

OpenAIRE

Renman, Niklas

2016-01-01

TIIVISTELMÄ Renman, Niklas. Tytöt pelitoiminnallisessa nuorisotyössä: Helsingin nuorisoasiainkeskuksen Pelitalon tyttökävijöiden kokemuksia toiminnasta. Helsinki, kevät 2016, 66 sivua, 1 liite. Diakonia-ammattikorkeakoulu, sosiaalialan koulutusohjelma, Sosionomi (AMK) + kirkon nuorisotyönohjaajan virkakelpoisuus. Opinnäytetyön tarkoituksena oli kartoittaa tyttöjen asenteita, toiveita ja koke-muksia pelitoiminnallisesta nuorisotyöstä. Tavoitteena oli selvittää, mitkä tekijät vaikuttava...

Mini-KiSS Online: an Internet-based intervention program for parents of young children with sleep problems – influence on parental behavior and children's sleep

Directory of Open Access Journals (Sweden)

Schlarb AA

2012-03-01

Full Text Available Angelika A Schlarb1,2,*, Isabel Brandhorst1,* 1University of Tuebingen, Faculty of Science, Department of Psychology, Tuebingen, 2University of Koblenz-Landau, Department of Psychology, Landau, Germany*The authors contributed equally to this workPurpose: Behavioral sleep problems are highly common in early childhood. These sleep problems have a high tendency to persist, and they may have deleterious effects on early brain development, attention, and mood regulation. Furthermore, secondary effects on parents and their relationship are documented. Negative parental cognition and behavior have been found to be important influencing factors of a child's behavioral sleep problems. Therefore, in the current study we examined the acceptance and efficacy of a newly developed Internet-based intervention program called Mini-KiSS Online for sleep disturbances for children aged 6 months to 4 years and their parents.Patients and methods: Fifty-five children (54.54% female; aged 8–57 months suffering from psychophysiological insomnia or behavioral insomnia participated in the 6-week online treatment. Sleep problems and treatment acceptance were examined with a sleep diary, anamnestic questionnaires, a child behavior checklist (the Child Behavior Checklist 1.5–5, and treatment evaluation questionnaires.Results: The evaluation questionnaires showed a high acceptance of Mini-KiSS Online. Parents would recommend the treatment to other families, were glad to participate, and reported that they were able to deal with sleep-related problems of their child after Mini-KiSS Online. Parental behavior strategies changed with a reduction of dysfunctional strategies, such as staying or soothing the child until they fell asleep, allowing the child to get up again and play or watch TV, or reading them another bedtime story. Frequency and duration of night waking decreased as well as the need for external help to start or maintain sleep. All parameters changed

Evaluation of liquid metal embrittlement of SS304 by Cd and Cd-Al solutions

International Nuclear Information System (INIS)

Thomas, J.K.; Iyer, N.C.; Begley, J.A.

1992-01-01

The susceptibility of stainless steel 304 to liquid metal embrittlement (LME) by cadmium (Cd) and cadmium-aluminum (Cd-Al) solutions was examined as part of a failure evaluation for SS304-clad cadmium reactor safety rods which had been exposed to elevated temperatures. The active, or cadmium (Cd) bearing, portion of the safety rod consists of a 0.756 in. diameter aluminum allow (Al-6061) core, a 0.05 in. thick Cd layer, and a 0.042 in. thick Type 304 stainless steel cladding. The safety rod thermal tests were conducted as part of a program to define the response of reactor core components to a hypothetical LOCA for the Savannah River Site (SRS) production reactor. LME was considered as a potential failure mechanism based on the nature of the failure and susceptibility of austenitic stainless steels to embrittlement by other liquid metals

Wavelet Based Hilbert Transform with Digital Design and Application to QCM-SS Watermarking

Directory of Open Access Journals (Sweden)

S. P. Maity

2008-04-01

Full Text Available In recent time, wavelet transforms are used extensively for efficient storage, transmission and representation of multimedia signals. Hilbert transform pairs of wavelets is the basic unit of many wavelet theories such as complex filter banks, complex wavelet and phaselet etc. Moreover, Hilbert transform finds various applications in communications and signal processing such as generation of single sideband (SSB modulation, quadrature carrier multiplexing (QCM and bandpass representation of a signal. Thus wavelet based discrete Hilbert transform design draws much attention of researchers for couple of years. This paper proposes an (i algorithm for generation of low computation cost Hilbert transform pairs of symmetric filter coefficients using biorthogonal wavelets, (ii approximation to its rational coefficients form for its efficient hardware realization and without much loss in signal representation, and finally (iii development of QCM-SS (spread spectrum image watermarking scheme for doubling the payload capacity. Simulation results show novelty of the proposed Hilbert transform design and its application to watermarking compared to existing algorithms.

Simultaneous radio and x-ray activity in SS 433

International Nuclear Information System (INIS)

Seaquist, E.R.; Gilmore, W.S.; Johnston, K.J.; Grindlay, J.E.

1982-01-01

Simultaneous observations at radio and X-ray wavelengths of flarelike events in SS 433 were made during 1979 October 5-9 and 19-22. The radio spectra show evidence for low-frequency absorption, which may be due to either synchrotron self-absorption or free-free absorption by a stellar wind. In the latter case, a model is developed indicating that clouds of relativistic gas are generated at least 10 14 cm from the stellar object and are swept outward through the stellar wind by highly collimated beams. A mass loss of approx.10 -5 M/sub sun/ yr -1 and an outflow speed of approx.1000 km s -1 are consistent with the data. The beam velocities (0.26c) are found to be consistent with the previous optical and radio data. The X-ray emission is evidently nonthermal and closely related to the radio flares, particularly during the event on October 5-9. The behavior during the second event on October 19-22 is considerably more complex. The source of the X-ray radiation is either synchrotron or inverse Compton emission. No clear cut decision is permitted by the data, although the latter mechanism seems to be an inevitable consequence of the known presence of both ultrarelativistic electrons and a high optical stellar luminosity. The inverse Compton mechansim is considered in more detail in the context of the model used to explain the radio behavior. The complexity of the October 19-22 data seems to defy' any simple model

“Höss, Stroop y Stangl: verdugos profesionales. Estudio comparativo sobre los perpetradores del Holocausto a la luz de sus propias palabras.”

Directory of Open Access Journals (Sweden)

Martín Félix Romero

2016-03-01

Full Text Available El presente trabajo es un estudio comparativo sobre perpetradores del Holocausto. Luego de una breve introducción sobre el fenómeno nazi en su contexto, se analizan los casos de tres de sus protagonistas: Rudolf Höss, Jürgen Stroop y Franz Stangl; comandante del campo de exterminio Auschwitz, responsable de la liquidación del gueto de Varsovia y comandante del campo de exterminio de Treblinka respectivamente. El caso de Höss es estudiado a partir de su autobiografía, mientras que los dos restantes a partir de las entrevistas e investigaciones de Kazimierz Moczarski y Gitta Sereny. Höss, Stangl y Stroop fueron condenados por las muertes de 1.100.000, entre 750.00 y 900.00 y 350.000 personas respectivamente. En este sentido una premisa de este trabajo es que ninguno de ellos puede ser considerado hombre corriente o persona común; por el contrario siguiendo a Raul Hilberg se los piensa como sujetos totalmente identificados e impregnados de la cosmovisión y la ideología del nacionalsocialismo y las SS que desde un marco de referencia previo agresivo y violento eventualmente fueron transformándose en asesinos en masa. Como sostiene Gustavo Cosacov estos sujetos serían dueños de una “santidad maligna o invertida” que había reemplazado sus valores éticos y morales cristiano-occidentales; permitiéndoles cometer crímenes en forma sistemática. A pesar de que la cuestión de los victimarios ha sido menos abordada que la de las victimas, en el campo historiográfico existen sólidos aportes. Basta nombrar a Christopher Browning, Peter Longerich o Raul Hilberg para explicitar la corriente de investigación a la que este texto reconoce y aspira. En este sentido el objetivo de las siguientes páginas primero es describir y explicar algunos de los mecanismos históricos, psicológicos y sociológicos que producen a perpetradores de asesinatos en serie de personas tan distantes como desconocidas; como así descifrar a también su marco de

Ray Next-Event Estimator Transport of Primary and Secondary Gamma Rays

Science.gov (United States)

2011-03-01

McGraw-Hill. Choppin, G. R., Liljenzin, J.-O., & Rydberg, J. (2002). Radiochemistry and Nuclear Chemistry (3rd ed.). Woburn, MA: Butterworth- Heinemann ...time-energy bins. Any performance enhancements (maybe parallel searching?) to the search routines decrease estimator computational time

Innocent or guilty? Redox activity in and magnetic and optical ...

Indian Academy of Sciences (India)

Unknown

The concept of ligand 'innocence' in chemistry was introduced by C K Jørgensen in the ..... region, between 700 and 900 nm, both being enhanced LMCT processes. ..... A 1631; (c) Sellmann D, Emig S and Heinemann F 1997 Angew. Chem.

Influence of helium-injection schedule and prior thermomechanical treatment on the microstructure of Type 316 SS

International Nuclear Information System (INIS)

Kohyama, A.; Ayrault, G.; Turner, A.P.L.

1982-10-01

The influence of different helium-injection schedules on microstructure development Ni + ion irradiated 316 SS at 625 0 C is discussed. Injection schedules were chosen to (1) approximate the magnetic fusion reactor condition and (2) mimic the mixed-spectrum reactor condition. Dual-ion irradiation to 25 dpa produced strongly bimodal cavity size distributions in solution-annealed and solution-annealed and aged samples, whereas single-ion irradiation followed by dual-ion irradiation to the same dose produced a cavity size distribution with a substantial component of intermediate-size cavities. Dual-ion irradiation produced only very small cavities in 20% CW material, while single-ion followed by dual-ion irradiation produced some intermediate size cavities and greater swelling. 10 figures

[J. Tapio. Suljetut vaunut, eli, Kadonnutta aikaa etsimässä ; Jukka Kostiainen. Kinnises tõllas, ehk, Kadunud aega otsimas ; Tatu Vaaskivi. Maarjamaa] / Hannu Oittinen

Index Scriptorium Estoniae

Oittinen, Hannu, 1959-

2013-01-01

Arvustus: Tapio, J. Suljetut vaunut, eli, Kadonnutta aikaa etsimässä. Porvoo : J. Kostiainen, 2012 ; Kostiainen, Jukka. Kinnises tõllas, ehk, Kadunud aega otsimas / tõlkinud Ants Paikre. Tartu : Atlex, 2013 ; Vaaskivi, Tatu. Maarjamaa / tõlkinud Ants Paikre. Tallinn : Kultuurileht, 2013. (Loomingu raamatukogu)

The hold-time effects on the low cycle fatigue behaviors of 316 SS in PWR primary environment

International Nuclear Information System (INIS)

Lee, Junho; Hong, Jong-Dae; Seo, Myung-Gyu; Jang, Changheui

2015-01-01

The effects of the environments on fatigue life of the structural materials used in nuclear power plants (NPPs) were known to be significant according to the extensive test results. Accordingly, the fatigue analysis procedures and the design fatigue curves were proposed in the ASME Code. However, the implication that the existing ASME design fatigue curves did not sufficiently reflect the effect of the operation conditions of nuclear power plants emerged as an issue to be resolved. One of possible reasons to explain the discrepancy is that the laboratory test conditions do not represent the actual plant transients. Therefore, it is necessary to clarify the effects of light water environments on fatigue life while considering more plant-relevant transient conditions such as hold-time. For this reason, this study will focus on the fatigue life of type 316 stainless steel (SS) in the pressurized water reactor (PWR) environments while incorporating the hold-time during the low cycle fatigue (LCF) test in simulated PWR environments. The objective of this study is to characterize the effects of hold-time on the fatigue life of austenitic stainless steels in PWR environments in comparison with the existing fixed strain rate results. Low cycle fatigue life tests were conducted for the type 316 SS in 310 .deg. C air and simulated PWR environments. To simulate the heat-up and cool-down transient, sub-peak strain holding during the down-hill of strain amplitude was chosen. Currently, LCF tests with 60 seconds holding are in progress. The 0.4, 0.04%/s strain rate condition test results are presented in this study, which shows somewhat longer fatigue life

The hold-time effects on the low cycle fatigue behaviors of 316 SS in PWR primary environment

Energy Technology Data Exchange (ETDEWEB)

Lee, Junho; Hong, Jong-Dae; Seo, Myung-Gyu; Jang, Changheui [KAIST, Daejeon (Korea, Republic of)

2015-05-15

The effects of the environments on fatigue life of the structural materials used in nuclear power plants (NPPs) were known to be significant according to the extensive test results. Accordingly, the fatigue analysis procedures and the design fatigue curves were proposed in the ASME Code. However, the implication that the existing ASME design fatigue curves did not sufficiently reflect the effect of the operation conditions of nuclear power plants emerged as an issue to be resolved. One of possible reasons to explain the discrepancy is that the laboratory test conditions do not represent the actual plant transients. Therefore, it is necessary to clarify the effects of light water environments on fatigue life while considering more plant-relevant transient conditions such as hold-time. For this reason, this study will focus on the fatigue life of type 316 stainless steel (SS) in the pressurized water reactor (PWR) environments while incorporating the hold-time during the low cycle fatigue (LCF) test in simulated PWR environments. The objective of this study is to characterize the effects of hold-time on the fatigue life of austenitic stainless steels in PWR environments in comparison with the existing fixed strain rate results. Low cycle fatigue life tests were conducted for the type 316 SS in 310 .deg. C air and simulated PWR environments. To simulate the heat-up and cool-down transient, sub-peak strain holding during the down-hill of strain amplitude was chosen. Currently, LCF tests with 60 seconds holding are in progress. The 0.4, 0.04%/s strain rate condition test results are presented in this study, which shows somewhat longer fatigue life.

Interacting large-scale magnetic fields and ionized gas in the W50/SS433 system

Science.gov (United States)

Farnes, J. S.; Gaensler, B. M.; Purcell, C.; Sun, X. H.; Haverkorn, M.; Lenc, E.; O'Sullivan, S. P.; Akahori, T.

2017-06-01

The W50/SS433 system is an unusual Galactic outflow-driven object of debatable origin. We have used the Australia Telescope Compact Array to observe a new 198 pointing mosaic, covering 3° × 2°, and present the highest-sensitivity full-Stokes data of W50 to date using wide-field, wide-band imaging over a 2 GHz bandwidth centred at 2.1 GHz. We also present a complementary Hα mosaic created using the Isaac Newton Telescope Photometric Hα Survey of the Northern Galactic Plane. The magnetic structure of W50 is consistent with the prevailing hypothesis that the nebula is a reanimated shell-like supernova remnant (SNR), which has been re-energized by the jets from SS433. We observe strong depolarization effects that correlate with diffuse Hα emission, likely due to spatially varying Faraday rotation measure (RM) fluctuations of ≥48-61 rad m-2 on scales ≤4.5-6 pc. We also report the discovery of numerous, faint, Hα filaments that are unambiguously associated with the central region of W50. These thin filaments are suggestive of an SNR's shock emission, and almost all have a radio counterpart. Furthermore, an RM-gradient is detected across the central region of W50, which we interpret as a loop magnetic field with a symmetry axis offset by ≈90° to the east-west jet-alignment axis, and implying that the evolutionary processes of both the jets and the SNR must be coupled. A separate RM-gradient is associated with the termination shock in the eastern ear, which we interpret as a ring-like field located where the shock of the jet interacts with the circumstellar medium. Future optical observations will be able to use the new Hα filaments to probe the kinematics of the shell of W50, potentially allowing for a definitive experiment on W50's formation history.

Observations of secular changes in the kinematic model of SS433

International Nuclear Information System (INIS)

Collins, G.W. II; Newsom, G.H.

1982-01-01

In this paper the authors present evidence that several of the defining parameters of the Kinematic Model for SS433 are not constant but rather exhibit long term systematic changes. Recent data confirm the existence of the previously reported decrease in the precessional period. The value for this period change, when combined with the observed change in the period of the synodic spectral variations, implies that the orbital period is not significantly changing on a time scale less than 1000 years. In addition they find mounting evidence for a statistically significant (4sigma) secular change in the cone angle theta at a rate of about -1.5 x 10 -3 deg/day. However, the surprisingly short time scales implied by the observed values of dP/dt and dtheta/dt when combined with estimates of the system age suggest the possible existence of detectable higher time derivatives. This view is supported by the most recent data which suggest a value for d 2 P/dt 2 approximately 10 -5 (days) -1 . It is possible to understand these secular changes in terms of the motions to be expected from an object exhibiting classical precession in response to an external torque. (Auth.)

A cognitive robotics system: the symbolic and sub-symbolic robotic intelligence control system (SS-RICS)

Science.gov (United States)

Kelley, Troy D.; Avery, Eric

2010-04-01

This paper will detail the progress on the development of the Symbolic and Subsymbolic Robotics Intelligence Control System (SS-RICS). The system is a goal oriented production system, based loosely on the cognitive architecture, the Adaptive Control of Thought-Rational (ACT-R) some additions and changes. We have found that in order to simulate complex cognition on a robot, many aspects of cognition (long term memory (LTM), perception) needed to be in place before any generalized intelligent behavior can be produced. In working with ACT-R, we found that it was a good instantiation of working memory, but that we needed to add other aspects of cognition including LTM and perception to have a complete cognitive system. Our progress to date will be noted and the challenges that remain will be addressed.

Crystal Structure of a CRISPR RNA-guided Surveillance Complex Bound to a ssDNA Target

Energy Technology Data Exchange (ETDEWEB)

Mulepati, Sabin [Johns Hopkins Univ., Baltimore, MD (United States); Heroux, Annie; Bailey, Scott [Johns Hopkins Univ., Baltimore, MD (United States)

2014-09-19

In prokaryotes, RNA derived from type I and type III CRISPR loci direct large ribonucleoprotein complexes to destroy invading bacteriophage and plasmids. In Escherichia coli, this 405-kilodalton complex is called Cascade. We report the crystal structure of Cascade bound to a single-stranded DNA (ssDNA) target at a resolution of 3.03 angstroms. The structure reveals that the CRISPR RNA and target strands do not form a double helix but instead adopt an underwound ribbon-like structure. This noncanonical structure is facilitated by rotation of every sixth nucleotide out of the RNA-DNA hybrid and is stabilized by the highly interlocked organization of protein subunits. These studies provide insight into both the assembly and the activity of this complex and suggest a mechanism to enforce fidelity of target binding.

Дополнения и уточнения к фауне чешуекрылых (Lepidoptera) Тульской области. 6 = Additions and corrections to the fauna of Lepidoptera of the Tula Province. 6

NARCIS (Netherlands)

Bolshakov, A.V. Sviridov, E.J. van Nieukerken, S.A. Ryabov L.V.; Sviridov, A.V.; Nieukerken, van E.J.; Ryabov, S.A.

2016-01-01

Additions and corrections to the lists of Lepidoptera of Tula Province are given, comprising 33 species, 23 of which are listed for the province for the first time, incl. 6 species (Stigmella obliquella (Heinemann, 1862), Phyllonorycter nicellii (Stainton, 1851), Insalebria serraticornella (Zeller,

Serodiagnosis of sporotrichosis infection in cats by enzyme-linked immunosorbent assay using a specific antigen, SsCBF, and crude exoantigens.

Science.gov (United States)

Fernandes, Geisa Ferreira; Lopes-Bezerra, Leila Maria; Bernardes-Engemann, Andréa Reis; Schubach, Tânia Maria Pacheco; Dias, Maria Adelaide Galvão; Pereira, Sandro Antonio; de Camargo, Zoilo Pires

2011-01-27

The main objective of this study is to standardize an ELISA for the diagnosis of feline sporotrichosis. Sporothrix schenckii is the etiological agent of human and animal sporotrichosis. Cats may act as reservoirs for S. schenckii and can transmit the infection to humans by a bite or scratch. There are few methods for the serological diagnosis of fungal diseases in animals. In this paper, an ELISA test for the diagnosis of cat sporotrichosis is proposed, which detects S. schenckii-specific antibodies in feline sera. Two different kinds of antigens were used: "SsCBF", a specific molecule from S. schenckii that consists of a Con A-binding fraction derived from a peptido-rhamnomannan component of the cell wall, and a S. schenckii crude exoantigen preparation. The ELISA was developed, optimized, and evaluated using sera from 30 cats with proven sporotrichosis (by culture isolation); 22 sera from healthy feral cats from a zoonosis center were used as negative controls. SsCBF showed 90% sensitivity and 96% specificity in ELISA; while crude exoantigens demonstrated 96% sensitivity and 98% specificity. The ELISA assay described here would be a valuable screening tool for the detection of specific S. schenckii antibodies in cats with sporotrichosis. The assay is inexpensive, quick to perform, easy to interpret, and permits the diagnosis of feline sporotrichosis. Copyright © 2010 Elsevier B.V. All rights reserved.

Some haltuun : Murrosikäisen sosiaalisen median käytön ohjaus terveydenhoitajan työssä

OpenAIRE

Paavola, Pinja; Haapala, Lotta

2016-01-01

Opinnäytetyön tavoitteena oli lisätä terveydenhoitajien tietämystä sosiaalisesta mediasta, jotta he osaavat ohjata murrosikäisiä sosiaalisen median turvallisessa käytössä ja, että murrosikäinen voi käyttää sosiaalista mediaa turvallisesti. Tarkoituksena oli selvittää, miten murrosikäinen käyttää sosiaalisen median sovelluksia ja miten terveydenhoitaja voisi ohjata sosiaalisen median turvalliseen käyttöön. Tutkimuskysymyksinä oli selvittää; mitä murrosikäinen tietää sosiaalisen median turvalli...

Fabrication of Nb3Al superconducting wires by utilizing the mechanically alloyed Nb(Al)ss supersaturated solid-solution with low-temperature annealing

International Nuclear Information System (INIS)

Pan, X.F.; Yan, G.; Qi, M.; Cui, L.J.; Chen, Y.L.; Zhao, Y.; Li, C.S.; Liu, X.H.; Feng, Y.; Zhang, P.X.; Liu, H.J.

2014-01-01

Highlights: • This paper reported superconducting properties of the powder-in-tube Nb 3 Al wires. • The Nb 3 Al wires were made by using Nb(Al) ss supersaturated solid solution powders. • The Cu-matrix Nb 3 Al superconducting wires have been successfully fabricated. • The transport J c of Nb 3 Al wires at 4.2 K, 10 T is up to 12,700 A/cm 2 . - Abstract: High-performance Nb 3 Al superconducting wire is a promising candidate to the application of high-field magnets. However, due to the production problem of km-grade wires that are free from low magnetic field instability, the Nb 3 Al wires made by rapid heating, quenching and transformation (RHQT) are still not available to the large-scale engineering application. In this paper, we reported the properties of the in situ powder-in-tube (PIT) Nb 3 Al superconducting wires, which were made by using the mechanically alloyed Nb(Al) ss supersaturated solid solution, as well as the low temperature heat-treatment at 800 °C for 10 h. The results show that Nb 3 Al superconductors in this method possess very fine grains and well superconducting properties, though a little of Nb 2 Al and Nb impurities still keep being existence at present work. At the Nb 3 Al with a nominal 26 at.% Al content, the onset T c reaches 15.8 K. Furthermore, a series of Nb 3 Al wires and tapes with various sizes have been fabricated; for the 1.0 mm-diameter wire, the J c at 4.2 K, 10 T and 14 T have achieved 12,700 and 6900 A/cm 2 , respectively. This work suggests it is possible to develop high-performance Cu-matrix Nb 3 Al superconducting wires by directly using the Nb(Al) ss supersaturated solid-solution without the complex RHQT heat-treatment process

Non-Invasive Detection of Lactate as a Biomarker of Response using Spectral Selective Multiple Quantum Editing Sequence (SS-SelMQC)

Science.gov (United States)

2013-11-01

phantoms with known concentrations (Lac with without Gd-DTPA doping , and Lac/Lip solutions). Once the RF coils are optimized, signal to noise (SNR...cylindrical phantoms (10mm and 15mm diameter) with 5, 15 and 30 mM lactate/ saline doped with and without 25 μΜ Gd-DPTA solutions. For Lac/Lip...approach to lactate production in human skeletal muscle during exercise. Med Sci Sports Exerc 2000;32:756–63. 51. Maekawa M, Sudo K, Li SS, Kanno T

Antibodies to SS-A/Ro-52kD and centromere in autoimmune liver disease: a clue to diagnosis and prognosis of primary biliary cirrhosis.

Science.gov (United States)

Granito, A; Muratori, P; Muratori, L; Pappas, G; Cassani, F; Worthington, J; Ferri, S; Quarneti, C; Cipriano, V; de Molo, C; Lenzi, M; Chapman, R W; Bianchi, F B

2007-09-15

Primary biliary cirrhosis (PBC) may be associated with various rheumatological disorders. To investigate the frequency and significance of 'rheumatological' antinuclear antibodies in the field of autoimmune chronic liver disease, with special regard to PBC. We studied 105 patients with PBC, 162 autoimmune liver disease controls (type 1 and 2 autoimmune hepatitis, primary sclerosing cholangitis), 30 systemic lupus erythematosus and 50 blood donors. Sera were tested for the presence of antibodies to extractable nuclear antigens (anti-ENA) by counterimmunoelectrophoresis, enzyme-linked and immunoblot (IB) assay, and for the presence of anti-centromere antibodies (ACA) by indirect immunofluorescence on HEp-2 cells and IB. The overall prevalence of IB-detected anti-ENA in PBC (30%) was higher than in type 1 autoimmune hepatitis (2.5%, P < 0.0001), type 2 autoimmune hepatitis (0%, P < 0.0001) and primary sclerosing cholangitis (11.5%, P = 0.006) and lower than in systemic lupus erythematosus (53%, P = 0.03). The most frequent anti-ENA reactivity in PBC was anti-SSA/Ro-52kD (28%). ACA were detected by IB in 21% PBC patients and never in the other subjects (P < 0.0001). Anti-SS-A/Ro/52kD positive PBC patients had at the time of diagnosis a more advanced histological stage (P = 0.01) and higher serum levels of bilirubin (P = 0.01) and IgM (P = 0.03) compared with negative ones. In the autoimmune liver disease setting, anti-SS-A/Ro-52kD and ACA have a high specificity for PBC and can thus be of diagnostic relevance in anti-mitochondrial antibodies negative cases. If confirmed in further studies with adequate follow-up, anti-SS-A/Ro-52kD antibodies might identify PBC patients with a more advanced and active disease.

Influence of agitation speeds and aeration rates on the Xylanase activity of Aspergillus niger SS7

Directory of Open Access Journals (Sweden)

Yasser Bakri

2011-08-01

Full Text Available In this study, the effect of agitation and aeration rates on xylanase activity of Aspergillus niger SS7 in 3-litre stirred tank bioreactor was investigated. The agitation rates tested were 100, 200 and 300 rpm at each airflow rates of 0.5, 1.0 and 1.5 vvm. The maximum xylanase activity in mono- agitator system was at the agitation speed of 200 rpm and aeration rate of 1.0 vvm. In bi-agitator system, at low agitation speed (100 rpm, the xylanase activity was enhanced by 13% compared to mono- agitator system for an aeration rate of 1.0 vvm. Xylanase productivity in continuous culture was higher by approximately 3.5 times than in batch culture.

African Journal of Biotechnology - Vol 13, No 34 (2014)

African Journals Online (AJOL)

Use of spent compost in the cultivation of Agaricus blazei · EMAIL FREE FULL TEXT EMAIL FREE FULL TEXT DOWNLOAD FULL TEXT DOWNLOAD FULL TEXT. GM Favara, C Sales-Campos, MT de Almeida Minhoni, OAAP Siqueira, MCN de Andrade. http://dx.doi.org/10.5897/AJB2014.13978 ...

Preliminary assessment of using tree-tissue analysis and passive-diffusion samplers to evaluate trichloroethene contamination of ground water at Site SS-34N, McChord Air Force Base, Washington, 2001

Science.gov (United States)

Cox, S.E.

2002-01-01

Two low-cost innovative sampling procedures for characterizing trichloroethene (TCE) contamination in ground water were evaluated for use at McChord Air Force Base (AFB) by the U.S. Geological Survey, in cooperation with the U.S. Air Force McChord Air Force Base Installation Restoration Program, in 2001. Previous attempts to characterize the source of ground-water contamination in the heterogeneous glacial outwash aquifer at McChord site SS-34N using soil-gas surveys, direct-push exploration, and more than a dozen ground-water monitoring wells have had limited success. The procedures assessed in this study involved analysis of tree-tissue samples to map underlying ground-water contamination and deploying passive-diffusion samplers to measure TCE concentrations in existing monitoring wells. These procedures have been used successfully at other U.S. Department of Defense sites and have resulted in cost avoidance and accelerated site characterization. Despite the presence of TCE in ground water at site SS-34N, TCE was not detected in any of the 20 trees sampled at the site during either early spring or late summer sampling. The reason the tree tissue procedure was not successful at the McChord AFB site SS-34N may have been due to an inability of tree roots to extract moisture from a water table 30 feet below the land surface, or that concentrations of TCE in ground water were not large enough to be detectable in the tree tissue at the sampling point. Passive-diffusion samplers were placed near the top, middle, and bottom of screened intervals in three monitoring wells and TCE was observed in all samplers. Concentrations of TCE from the passive-diffusion samplers were generally similar to concentrations found in samples collected in the same wells using conventional pumping methods. In contrast to conventional pumping methods, the collection of ground-water samples using the passive-diffusion samples did not generate waste purge water that would require hazardous

Student and Teacher Interventions: A Framework for Analysing Mathematical Discourse in the Classroom

Science.gov (United States)

Drageset, Ove Gunnar

2015-01-01

Mathematical discourse in the classroom has been conceptualised in several ways, from relatively general patterns such as initiation-response-evaluation (Cazden in "Classroom discourse: the language of teaching and learning," Heinemann, London, 1988; Mehan in "Learning lessons: social organization in the classroom." Cambridge,…

Gelechiidae II (Gelechiinae: Gnorimoschemini)

DEFF Research Database (Denmark)

Huemer, Peter; Karsholt, Ole

argentinae (Povolný, 1989) syn. n. of Phthorimaea operculella (Zeller, 1873); Opacopsis buvati Povolný, 1992 syn. n. of Ephysteris deserticolella (Staudinger, 1871); Doryphora? praticolella Christoph, 1872 as ssp. , syn. n. and Opacopsis gallica Povolný, 1992 syn. n. of Ephysteris insulella (Heinemann, 1870...

JUNE 2010.for Ogidi (final).cdr

African Journals Online (AJOL)

User

and creatinine, urinalysis, liver function test and intravenous urography were normal. Apart from her packed cell volume of ... of epithelial ovarian tumours accounting for approximately 10-20% of these neoplasia . About 75 ... Heinemann Educational Books (Nig.) Plc;. 2006: 197-217. Benign and malignant ovarian masses.

Molecular Interactions of High Energy Fuels and Jet Fuels with Oncogenic Viruses and Endogenous Viruses.

Science.gov (United States)

1981-10-01

Aspergillus Oryzae . Biochimica et Biophysica Acta II4:158-168. 2) Benveniste, R.E., Heinemann, R., Wilson, G.L., Callahan, R. and Todaro, G.J. 1974...Some properties are listed below: Table 5. Characteristics of Asbestos Fibers.1 Characteristics Chrysotile Crocidolite Amosite Base Composition Hydrate

PN Sequence Preestimator Scheme for DS-SS Signal Acquisition Using Block Sequence Estimation

Directory of Open Access Journals (Sweden)

Sang Kyu Park

2005-03-01

Full Text Available An m-sequence (PN sequence preestimator scheme for direct-sequence spread spectrum (DS-SS signal acquisition by using block sequence estimation (BSE is proposed and analyzed. The proposed scheme consists of an estimator and a verifier which work according to the PN sequence chip clock, and provides not only the enhanced chip estimates with a threshold decision logic and one-chip error correction among the first m received chips, but also the reliability check of the estimates with additional decision logic. The probabilities of the estimator and verifier operations are calculated. With these results, the detection, the false alarm, and the missing probabilities of the proposed scheme are derived. In addition, using a signal flow graph, the average acquisition time is calculated. The proposed scheme can be used as a preestimator and easily implemented by changing the internal signal path of a generally used digital matched filter (DMF correlator or any other correlator that has a lot of sampling data memories for sampled PN sequence. The numerical results show rapid acquisition performance in a relatively good CNR.