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Sample records for blazei ss heinemann

  1. Cytotoxicity and genotoxicity of Agaricus blazei methanolic extract fractions assessed using gene and chromosomal mutation assays

    OpenAIRE

    Marilanda Ferreira Bellini; Leonardo Neves Cabrioti; Ana Paula Terezan; Berenice Quinzani Jordão; Lúcia Regina Ribeiro; Mário Sérgio Mantovani

    2008-01-01

    Functional food investigations have demonstrated the presence of substances that could be beneficial to human health when consumed. However, the toxic effects of some substances contained in foods have been determined. Reported medicinal and nutritive properties have led to the extensive commercialization of the basidiomycete fungi Agaricus blazei Murrill (sensu Heinemann), also known as Agaricus brasiliensis Wasser et al., Agaricus subrufescens Peck or the Brazilian medical mushroom (BMM). D...

  2. Cytotoxicity and genotoxicity of Agaricus blazei methanolic extract fractions assessed using gene and chromosomal mutation assays

    Directory of Open Access Journals (Sweden)

    Marilanda Ferreira Bellini

    2008-01-01

    Full Text Available Functional food investigations have demonstrated the presence of substances that could be beneficial to human health when consumed. However, the toxic effects of some substances contained in foods have been determined. Reported medicinal and nutritive properties have led to the extensive commercialization of the basidiomycete fungi Agaricus blazei Murrill (sensu Heinemann, also known as Agaricus brasiliensis Wasser et al., Agaricus subrufescens Peck or the Brazilian medical mushroom (BMM. Different methanolic extract fractions (ME of this mushroom were submitted to the cytokinesis-block micronucleus (CBMN clastogenic assay and the hypoxanthine-guanine phosphoribosyl transferase locus (HGPRT assay for gene mutation, both using Chinese hamster ovary cells clone K1 (CHO-K1. The results suggest that all the fractions tested possess cytotoxic and mutagenic potential but no clastogenic effects. Further information is needed on the biochemical components of the A. blazei methanol fractions to identify any substances with cytotoxic and/or mutagenicity potential. These findings indicate that A. blazei methanolic extract should not be used due to their genotoxicity and care should be taken in the use of A. blazei by the general population until further biochemical characterization of this fungi is completed.

  3. Cultivation of Agaricus blazei on Pleurotus spp. spent substrate

    OpenAIRE

    Regina Maria Miranda Gern; Nelson Libardi Junior; Gabriela Nunes Patrício; Elisabeth Wisbeck; Mariane Bonatti Chaves; Sandra Aparecida Furlan

    2010-01-01

    The aim of this work was the use of Pleurotus ostreatus and Pleurotus sajor-caju for the previous lignocellulolytic decomposition of banana tree leaf straw and the further use of the degraded straw as substrate for the culture of Agaricus blazei. For optimising the production of A. blazei in terms of yield (Y%) and biological efficiency (BE%), adjustments to the composition of the substrate were evaluated in a 2(5) experimental design. The following components were tested in relation to % of ...

  4. 巴氏菇Agaricus blazei murrill栽培新法初步研究%PRELIMINARY STUDIES ON NEW METHOD FOR CULTIUATING AGARICUS BLAZEI MURRILL

    Institute of Scientific and Technical Information of China (English)

    杨旭

    2000-01-01

    A technique for cultivating agaricus blazei murrill is reported. The technique used bagasse, in stead of muck as material, and through the secondary fermention of cultivated material to cultivate.%本文报导了以苷蔗渣为原料,不用粪肥,经栽培料二次发酵生产agaricus blazei murrill技术.

  5. Agaricus blazei Murill - immunomodulatory properties and health benefits

    OpenAIRE

    Biedron R; Tangen JM; Maresz K; Hetland G

    2012-01-01

    AbstractThe Agaricus blazei Murill (AbM), also known as Agaricus brasiliensis L. due to its origin in Brazilian rain forest, is an edible mushroom of the Basidiomycetes family, which also comprises medicinal mushrooms such as Hericium erinaceus and Grifola frondosa. AbM has been used in traditional medicine locally and also recently as a health food worldwide. Since it has been found to possess immunomodulatory properties, its biological and health-related effects, as well as its isolated ac...

  6. Cultivation of Agaricus blazei on Pleurotus spp. spent substrate

    Directory of Open Access Journals (Sweden)

    Regina Maria Miranda Gern

    2010-08-01

    Full Text Available The aim of this work was the use of Pleurotus ostreatus and Pleurotus sajor-caju for the previous lignocellulolytic decomposition of banana tree leaf straw and the further use of the degraded straw as substrate for the culture of Agaricus blazei. For optimising the production of A. blazei in terms of yield (Y% and biological efficiency (BE%, adjustments to the composition of the substrate were evaluated in a 2(5 experimental design. The following components were tested in relation to % of substrate dry mass: urea (1 and 10%, rice bran (10 or 20% or ammonium sulphate (0 or 10%, inoculum (10 or 20% and the casing material (subsoil or burned rice husks. The best results (79.71 Y% and 6.73 BE% were found when the substrate containing 10% of rice bran, without ammonium sulphate, inoculated with 20% and covered with subsoil was used.O cultivo de fungos comestíveis e medicinais utilizando resíduos da agroindústria vem se apresentando como uma alternativa econômica para o pequeno produtor rural, favorecendo a agricultura familiar do nordeste catarinense. Este trabalho avaliou o fungo Pleurotus para a decomposição lignocelulolítica de palha de folhas de bananeira e a utilização da palha residual como substrato para o cultivo de Agaricus blazei. Ajustes na composição do substrato residual de Pleurotus, tais como o tipo e a concentração da fonte de nitrogênio, a porcentagem de inóculo e a camada de cobertura, foram avaliadas. O substrato residual que mais favoreceu a produção de A. blazei em Eficiência Biológica (6,73%, Rendimento (79,71% e menor tempo para emissão do primeiro primórdio (27 dias foi o substrato residual de P. ostreatus inoculado com 20% de inóculo (ms, 10% de farelo de arroz (ms, sem sulfato de amônio e utilizando terra de subsolo como camada de cobertura.

  7. Agaricus blazei, supstrat za proizvodnju β-1,3-glukanaze s pomoću Trichoderma harzianum Rifai

    OpenAIRE

    Carneiro, Andréia A. J.; Giese, Ellen C.; Barbosa, Aneli M.; Gomes, Eleni; da Silva, Roberto

    2011-01-01

    Ekstrakt gljive Agaricus blazei (Agaricus brasiliensis) upotrijebljen je kao supstrat za proizvodnju β-1,3-glukanaze submerznim uzgojem plijesni Trichoderma harzianum Rifai. Centralno složeni 22 faktorijalni dizajn razvijen je primjenom ovih varijabla: vrijeme uzgoja (x1/dan) i koncentracija ekstrakta Agaricus blazei (x2/(g/L)), a rezultati su analizirani metodom odzivnih površina (RSM). Rezultati pokazuju da je koncentracija ekstrakta Agaricus blazei najvažnija varijabla za proizvodnju β-1,3...

  8. Ipomoea batatas and Agarics blazei ameliorate diabetic disorders with therapeutic antioxidant potential in streptozotocin-induced diabetic rats

    OpenAIRE

    Niwa, Atsuko; Tajiri, Takashi; Higashino, Hideaki

    2011-01-01

    Ipomoea batatas, Agaricus blazei and Smallanthus sonchifolius are known to favorably influence diabetes mellitus. To clarify their antidiabetic efficacy and hypoglycemic mechanisms, we treated streptozotocin-induced diabetic rats with daily oral feeding of powdered Ipomoea batatas (5 g kg−1 d−1), Agaricus blazei (1 g kg−1 d−1) or Smallanthus sonchifolius (4 g kg−1 d−1) for 2 months. Treatments with Ipomoea batatas or Agaricus blazei, but not Smallanthus sonchifolius, significantly suppressed ...

  9. IL-12 Production Induced by Agaricus blazei Fraction H (ABH) Involves Toll-like Receptor (TLR)

    OpenAIRE

    Kasai, H.; He, L. M.; Kawamura, M.; Yang, P. T.; Deng, X. W.; Munkanta, M.; Yamashita, A; H Terunuma; Hirama, M.; Horiuchi, I.; Natori, T.; Koga, T; Y. Amano; Yamaguchi, N.; Ito, M

    2004-01-01

    Agaricus blazei Murill is an edible fungus used in traditional medicine, which has various well-documented medicinal properties. In the present study, we investigated the effects of hemicellulase-derived mycelia extract (Agaricus blazei fraction H: ABH) on the immune system. First, we examined the cytokine-inducing activity of ABH on human peripheral mononuclear cells (PBMC). The results indicated that ABH induced expression of IL-12, a cytokine known to be a critical regulator of cellular im...

  10. Effect of different compounds on the induction of laccase production by Agaricus blazei.

    Science.gov (United States)

    Valle, J S; Vandenberghe, L P S; Oliveira, A C C; Tavares, M F; Linde, G A; Colauto, N B; Soccol, C R

    2015-01-01

    Laccases are polyphenol oxidases produced by many fungi and have many applications in textile, food and beverage, and pulp and paper industries. Laccase production can be induced using aromatic or phenolic compounds that mostly affect the transcription of laccase-encoding genes. In this study, we analyzed laccase and biomass production by Agaricus blazei in the presence of different concentrations of nitrogen, copper, and inducers such as pyrogallol, veratryl alcohol, xylidine, vanillin, guaiacol, and ethanol. Laccase production by A. blazei U2-4 reached 43.8 U/mL in the presence of 2.8 g/L nitrogen and 150 μM copper. However, addition of copper to the cultivation medium decreased biomass production. Different compounds differentially induced laccase production by A. blazei. Moreover, different concentrations of these inducers exerted different effects on laccase activity. Ethanol (1.0 mM), guaiacol (0.5 mM), and vanillin (0.5 mM) were the best inducers and increased laccase activity by 120% (A. blazei U2-2), 30% (A. blazei U2-3), and 9% (A. blazei U2-4), respectively. In contrast, pyrogallol and xylidine decreased laccase activity but increased biomass production. PMID:26634556

  11. Agaricus blazei Murill - immunomodulatory properties and health benefits

    Directory of Open Access Journals (Sweden)

    Biedron R

    2012-11-01

    Full Text Available AbstractThe Agaricus blazei Murill (AbM, also known as Agaricus brasiliensis L. due to its origin in Brazilian rain forest, is an edible mushroom of the Basidiomycetes family, which also comprises medicinal mushrooms such as Hericium erinaceus and Grifola frondosa. AbM has been used in traditional medicine locally and also recently as a health food worldwide. Since it has been found to possess immunomodulatory properties, its biological and health-related effects, as well as its isolated active ingredients e.g. beta-glucans, have been examined by scientists. Otherinvestigations have been performed with mixed mushroom products, such as AndoSanTM, which contains mostly AbM, but also the two other mushrooms above. AbM-related benefits reviewed here include effects against cancer, infections, inflammation, allergy/ asthma and diabetes. Effects of AndoSanTMand other AbM-based extracts have been compared in a bacterial sepsis model.

  12. Production flush of Agaricus blazei on Brazilian casing layers

    Directory of Open Access Journals (Sweden)

    Nelson Barros Colauto

    2011-06-01

    Full Text Available This study aimed to verify the biological efficiency and production flushes of Agaricus blazei strains on different casing layers during 90 cultivation days. Four casing layers were used: mixture of subsoil and charcoal (VCS, lime schist (LSC, São Paulo peat (SPP and Santa Catarina peat (SCP; and two genetically distant A. blazei strains. The fungus was grown in composted substratum and, after total colonization, a pasteurized casing layer was added over the substratum, and fructification was induced. Mushrooms were picked up daily when the basidiocarp veil was stretched, but before the lamella were exposed. The biological efficiency (BE was determined by the fresh basidiocarp mass divided by the substratum dry mass, expressed in percentage. The production flushes were also determined over time production. The BE and production flushes during 90 days were affected by the strains as well as by the casing layers. The ABL26 and LSC produced the best BE of 60.4%. Although VCS is the most used casing layer in Brazil, it is inferior to other casing layers, for all strains, throughout cultivation time. The strain, not the casing layer, is responsible for eventual variations of the average mushroom mass. In average, circa 50% of the mushroom production occurs around the first month, 30% in the second month, and 20% in third month. The casing layer water management depends on the casing layer type and the strain. Production flush responds better to water reposition, mainly with ABL26, and better porosity to LSC and SCP casing layers.

  13. Agaricus Blazei Hot Water Extract Shows Anti Quorum Sensing Activity in the Nosocomial Human PathogenPseudomonas Aeruginosa

    NARCIS (Netherlands)

    Sokovic, M.; Ciric, A.; Glamoclija, J.; Nicolic, M.; Griensven, van L.J.L.D.

    2014-01-01

    The edible mushroom Agaricus blazei Murill is known to induce protective immunomodulatory action against a variety of infectious diseases. In the present study we report potential anti-quorum sensing properties of A. blazei hot water extract. Quorum sensing (QS) plays an important role in virulence,

  14. Waffen-SS

    DEFF Research Database (Denmark)

    Christensen, Claus Bundgård

    Bogen analyserer SS-organisationens væbnede gren, Waffen-SS ud fra en række vinkler. De vigtigste er: 1) koblingen mellem Waffen-SS og resten af SS, 2) Waffen-SS som militær faktor i Det Tredje Rige, 3) Waffen-SS soldaternes politiske indoktrinering, 4) relationerne mellem de mange nationaliteter...

  15. Genetic characterization of isolates of the basidiomycete Agaricus blazei by RAPD

    Directory of Open Access Journals (Sweden)

    Colauto Nelson Barros

    2002-01-01

    Full Text Available The genetic divergence of five isolates of Agaricus blazei was determined based on RAPD data. Results indicate that there is little genetic variability among the commercialized strains and that RAPD is a feasible and low cost technique that can be used to characterize this fungus.

  16. Genetic characterization of isolates of the basidiomycete Agaricus blazei by RAPD

    OpenAIRE

    Colauto Nelson Barros; Dias Eustáquio Souza; Gimenes Marcos Aparecido; Eira Augusto Ferreira da

    2002-01-01

    The genetic divergence of five isolates of Agaricus blazei was determined based on RAPD data. Results indicate that there is little genetic variability among the commercialized strains and that RAPD is a feasible and low cost technique that can be used to characterize this fungus.

  17. Agaricus Blazei Hot Water Extract Shows Anti Quorum Sensing Activity in the Nosocomial Human Pathogen Pseudomonas Aeruginosa

    OpenAIRE

    Marina Soković; Ana Ćirić; Jasmina Glamočlija; Miloš Nikolić; Van Griensven, Leo J. L. D.

    2014-01-01

    The edible mushroom Agaricus blazei Murill is known to induce protective immunomodulatory action against a variety of infectious diseases. In the present study we report potential anti-quorum sensing properties of A. blazei hot water extract. Quorum sensing (QS) plays an important role in virulence, biofilm formation and survival of many pathogenic bacteria, including the Gram negative Pseudomonas aeruginosa, and is considered as a novel and promising target for anti-infectious agents. In thi...

  18. The Medicinal Mushroom Agaricus blazei Murrill: Review of Literature and Pharmaco-Toxicological Problems

    OpenAIRE

    F.Firenzuoli; Gori, L.; Lombardo, G.

    2007-01-01

    Agaricus blazei Murrill (ABM) popularly known as ‘Cogumelo do Sol’ in Brazil, or ‘Himematsutake’ in Japan, is a mushroom native to Brazil, and widely cultivated in Japan for its medicinal uses, so it is now considered as one of the most important edible and culinary-medicinal biotechnological species. It was traditionally used to treat many common diseases like atherosclerosis, hepatitis, hyperlipidemia, diabetes, dermatitis and cancer. In vitro and in vivo ABM has shown immunomodulatory and ...

  19. The Medicinal Values of Culinary-Medicinal Royal Sun Mushroom (Agaricus blazei Murrill)

    OpenAIRE

    Hang Wang; Zhiming Fu; Chunchao Han

    2013-01-01

    Agaricus blazei Murrill (ABM), a mushroom native to Brazil, is a basidiomycete brown fungus, which is popularly known as “Cogumelo do Sol” in Brazil or “Himematsutake” in Japan, and there has been a prominent increase in the use of ABM for therapeutic and medicinal purposes. ABM is useful against a variety of diseases like cancer, tumor, chronic hepatitis, diabetes, atherosclerosis, hypercholesterolemia, and so on. In this review, we demonstrated various pharmacological effects of ABM, so tha...

  20. Agarol, an ergosterol derivative from Agaricus blazei, induces caspase-independent apoptosis in human cancer cells.

    Science.gov (United States)

    Shimizu, Takamitsu; Kawai, Junya; Ouchi, Kenji; Kikuchi, Haruhisa; Osima, Yoshiteru; Hidemi, Rikiishi

    2016-04-01

    Agaricus blazei (A. blazei) is a mushroom with many biological effects and active ingredients. We purified a tumoricidal substance from A. blazei, an ergosterol derivative, and named it 'Agarol'. Cytotoxic effects of Agarol were determined by the MTT assay using A549, MKN45, HSC-3, and HSC-4 human carcinoma cell lines treated with Agarol. Apoptosis was detected by flow cytometry analysis. Reactive oxygen species (ROS) levels and mitochondria membrane potential (∆ψm) were also determined by flow cytometry. Western blot analysis was used to quantify the expression of apoptosis-related proteins. Agarol predominantly induced apoptosis in two p53-wild cell lines (A549 and MKN45) compared to the other p53-mutant cell lines (HSC-3 and HSC-4). Further mechanistic studies revealed that induction of apoptosis is associated with increased generation of ROS, reduced ∆ψm, release of apoptosis-inducing factor (AIF) from the mitochondria to the cytosol, upregulation of Bax, and downregulation of Bcl-2. Caspase-3 activities did not increase, and z-VAD-fmk, a caspase inhibitor, did not inhibit the Agarol-induced apoptosis. These findings indicate that Agarol induces caspase-independent apoptosis in human carcinoma cells through a mitochondrial pathway. The in vivo anticancer activity of Agarol was confirmed in a xenograft murine model. This study suggests a molecular mechanism by which Agarol induces apoptosis in human carcinoma cells and indicates the potential use of Agarol as an anticancer agent. PMID:26893131

  1. Composto exaurido do cogumelo Agaricus blazei na dieta de frangos de corte Spent mushroom substrate of Agaricus blazei in broiler chicks diet

    Directory of Open Access Journals (Sweden)

    Alexandre Magno Batista Machado

    2007-08-01

    Full Text Available Avaliou-se o efeito da substituição de um antibiótico por diferentes níveis do composto exaurido do cogumelo (CEC Agaricus blazei sobre o desempenho de frangos de corte de 1 a 42 dias de idade. Foram avaliadas sete dietas, seis com adição de CEC (0,0; 0,2; 0,4; 0,6; 0,8 e 1,0% e uma com antibiótico (avilamicina 10 ppm. Foram utilizados 588 pintos de 1 dia de idade, distribuídos em um delineamento inteiramente casualizado, com seis repetições por dieta (três por sexo e 14 aves por unidade experimental. As aves que não receberam qualquer aditivo na dieta apresentaram os piores resultados de desempenho. Níveis de CEC superiores a 0,4% ocasionaram redução gradual no desempenho. O nível de 0,2% de CEC proporcionou o maior ganho de peso e o de 0,21%, a melhor conversão alimentar e o maior consumo de ração. À exceção do rendimento de peito e do rendimento de gordura abdominal, os aditivos não influenciaram os rendimentos de carcaça e dos cortes. O composto exaurido do cogumelo Agaricus blazei pode ser utilizado como aditivo alternativo ao antibiótico no nível de 0,2% da dieta, pois não prejudicou o desempenho produtivo e o rendimento de carcaça das aves.The effect of the substitution of an antibiotic by different levels of spent mushroom substrate (SMS of Agaricus blazei on the performance of broiler chicks from 1 to 42 days old was evaluated. Seven diets were evaluated, six with SMS addiction (0.0, 0.2, 0.4, 0.6, 0.8, and 1.0% and one with antibiotic (avilamycin 10 ppm. A total of 588 one day old chicks was allotted to a complete randomized design, with six replications per diet (three per sex and 14 birds per experimental unit. The birds fed diet with any additive showed the worst performance results. Levels of SMS above 0.4% caused gradual reduction in the performance. The level of 0.2% of SMS provided the highest value of weight gain and of 0.21% promoted the best feed conversion and the highest value of feed intake

  2. Analgesic Activity of a Glucan Polysaccharide Isolated from Agaricus blazei Murill

    OpenAIRE

    Maria Leônia C. Gonzaga; Menezes, Thiago M. F.; de Souza, José R. R.; Nágila M. P. S. Ricardo; Ana L. P. Freitas; Sandra de A. Soares

    2013-01-01

    A water soluble polysaccharide and its selective precipitated fractions (F1, F2, and F3) were isolated from hot water extract of fruiting bodies of Agaricus blazei Murril known as Agaricus brasiliensis to evaluate the analgesic activity of the materials in Swiss mice. The isolated material and its fractions were characterized by two-dimensional COSY and HMQC spectra as β-(1→6)-α-(1→4)-glucan-bound-protein polysaccharide constituted mainly by β-(1→6) glucan. The glucan-protein polysaccharide a...

  3. Study on Several Characteristics of Agaricus blazei Murril Strain J3 Irradiated by 60Co

    Institute of Scientific and Technical Information of China (English)

    WENG Bo-qi; JIANG Zhi-he; HUANG Ting-jun; CHEN Jian; ZHENG Wei-wen

    2003-01-01

    A new mutant strain J3 from Agaricus blazei Murril was obtained by 60Co irradiation. The yields of successive generations were increased more than 70% to compare with their original strain. The component analysis on amino acids and fatty acid illuminated that the nutrient value of strain J3 fruiting body was better than the original strain. The apparent nodule structure was found in the hyphea of J3 strain through the observation by scanning electron microscope. RAPD analysis showed the great difference of PCR fingerprints between J3 and its original strain. It is a promising mutant strain for further commercial development in the future.

  4. Nutrient value protein of mutant J3 of agaricus blazei by irradiation

    International Nuclear Information System (INIS)

    The studies of nutrient value protein of mutant J3 of Agaricus blazei were carried out. The results showed that mutant J3 had 9 kinds of essential amino acids, the compositions of essential amino acids is 46.0% of total amino acids. The amino acid score is 94.5, the essential amino acid index is 92.4, biological value is 89.0, the ratio coefficient of amino acids is 72.3, the nutrient value is 32.5. The chemical score is 74.18. These results proved that the protein of mutant J3 had high nutrient value

  5. Phase I Clinical Study of the Dietary Supplement, Agaricus blazei Murill, in Cancer Patients in Remission

    OpenAIRE

    Satoshi Ohno; Yoshiteru Sumiyoshi; Katsuyoshi Hashine; Akitomi Shirato; Satoru Kyo; Masaki Inoue

    2011-01-01

    Although many cancer patients use complementary and alternative medicine, including Agaricus blazei Murill (ABM), safety is not yet well understood. Cancer survivors took 1.8, 3.6, or 5.4 g ABM granulated powder (Kyowa Wellness Co., Ltd., Tokyo, Japan) per day orally for 6 months. Adverse events were defined by subjective/objective symptoms and laboratory data according to the National Cancer Institute Common Terminology Criteria for Adverse Events version 3.0 (NCI-CTCAE v3.0). Seventy-eight ...

  6. Coloração de núcleos de esporos e hifas do cogumelo Agaricus blazei Nucleus stain of spores and hifas Ofagaricus blazei

    Directory of Open Access Journals (Sweden)

    Cláudia Regina Gontijo Labory

    2003-04-01

    Full Text Available Com o objetivo de se estudar o comportamento nuclear durante o ciclo sexual do cogumelo Agaricus blazei , técnicas de coloração e a utilização de fluorocromos foram testadas, visando a uma padronização metodológica para estudos citogenéticos futuros. Das técnicas avaliadas neste trabalho (método de Feulgen, coloração com Giemsa e fluorescência-DAPI, a coloração com Giemsa e o fluorocromo DAPI apresentaram os melhores resultados para evidenciar núcleos. O corante Giemsa permitiu a visualização de compartimentos multinucleados. Com o DAPI, não foi possível a visualização dos septos, sendo necessária a utilização adicional de calcofluor, que tem a propriedade de corar a quitina que delimita os compartimentos das hifas.In order to study the nuclear behavior during the sexual cycle of Agaricus blazei , some coloration and fluorescent stain techniques were tested for a method standardization for fut ure cytogenetical works. Among the studied techniques (Schiff reactive, Giemsa, and fluorescent stain - DAPI, the Giemsa and fluorescent DAPI technique provided the best results. With Giemsa stain, it was possible to visualize multinucleate cell compartments. It was not possible to stain compartmental delimitation with DAPI stain, which was possible only with an additional fluorescent stain - calcofluor, which is specific for chitin.

  7. Agaricus Blazei Hot Water Extract Shows Anti Quorum Sensing Activity in the Nosocomial Human Pathogen Pseudomonas Aeruginosa

    Directory of Open Access Journals (Sweden)

    Marina Soković

    2014-04-01

    Full Text Available The edible mushroom Agaricus blazei Murill is known to induce protective immunomodulatory action against a variety of infectious diseases. In the present study we report potential anti-quorum sensing properties of A. blazei hot water extract. Quorum sensing (QS plays an important role in virulence, biofilm formation and survival of many pathogenic bacteria, including the Gram negative Pseudomonas aeruginosa, and is considered as a novel and promising target for anti-infectious agents. In this study, the effect of the sub-MICs of Agaricus blazei water extract on QS regulated virulence factors and biofilm formation was evaluated against P. aeruginosa PAO1. Sub-MIC concentrations of the extract which did not kill P. aeruginosa nor inhibited its growth, demonstrated a statistically significant reduction of virulence factors of P. aeruginosa, such as pyocyanin production, twitching and swimming motility. The biofilm forming capability of P. aeruginosa was also reduced in a concentration-dependent manner at sub-MIC values. Water extract of A. blazei is a promising source of antiquorum sensing and antibacterial compounds.

  8. Genotoxic and antigenotoxic effects of organic extracts of mushroom Agaricus blazei Murrill on V79 cells

    Directory of Open Access Journals (Sweden)

    Guterres Zaira da Rosa

    2005-01-01

    Full Text Available Agaricus blazei Murrill, popularly known as the sun mushroom, is a native mushroom in SP, Brazil, that has been widely used in the treatment of cancer and many other pathologies in different parts of the world. A water-soluble protein-polysaccharide complex (1 -> 6beta-D-glucan has been isolated from its fruiting body that showed immune-modulation activity. From organic extracts, linoleic acid has been isolated and determined to be the main substance with antimutagenic activity. Using both the micronucleus (MN and comet (single cell microgel electrophoresis assays, this study determined the genotoxic and antigenotoxic potential of A. blazei (AB obtained from commercial sources or the following strains: a strains AB 97/29 (young and sporulated phases; b a mixture taken from AB 96/07, AB 96/09 and AB 97/11 strains; and c commercial mushrooms from Londrina, PR and Piedade, SP, designated as AB PR and AB SP, respectively. The extracts from these mushrooms were isolated in chloroform:methanol (3:1 and used in vitro at three different concentrations. V79 cells (Chinese hamster lung cells were exposed to the extracts under pre-, simultaneous and post-treatment conditions, combined with methyl methanesulfonate (MMS. Under the circumstances of this study, these organic extracts did not show any genotoxic or mutagenic effects, but did protect cells against the induction of micronuclei by MMS.

  9. Influence of structural features on immunostimulating activity of glucans extracted from Agaricus blazei mushroom

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    Kozarski Maja S.

    2009-01-01

    Full Text Available High molecular weight b-D-glucans derived from Basidiomycetes cell walls are able to specifically activate cellular and humoral components of the host immune system. The aim of this paper was to examine immunomodulating activity of native, chemically and enzimatically modified glucans from Agaricus blazei mushroom and to determine which structural features are of primary importance for their stimulation referring to humane immune cells. The immunomodulating activities were tested in vitro, by stimulation of peripheral blood mononuclear cells (PBMCs and measuring of interferon-gamma (IFN-g production by enzyme linked immunosorbent assay (ELISA. Measurements of immunomodulatory capacity of Agaricus blazei native glucans showed their expressive immunostimulating effect on activated PBMCs and synthesis of IFN-g. The results obtained after the stimulation of cells with 1M H2SO4 and 1M NaOH, the treated glucans showed that primary structure is of more importance than the tertiary structure of the triple helix for their immunostimulating activity and synthesis of IFN-g. Glucans of lower molecular weight obtained after acid hydrolysis appeared as effective immunostimulators of PBMC's. The results obtained after the incubation of cells with 1,6 b-glucanase modified glucans suggest that b-(1,6 binding of glucose monomers probably has no importance for the production of imunostimulating effects, in vitro. This confirmed that b-(1,3 bonds are the primary determinants of immunomodulatory activities and stimulation of IFN-g synthesis.

  10. SS-CUSUM Chart

    OpenAIRE

    Thaga Keoagile

    2009-01-01

    A cumulative sum (CUSUM) control chart referred to as the SS-CUSUM chart is proposed that is capable of simultaneously detecting changes in both mean and standard deviation. The construction of this chart is based on the sum of squares of the maximum standard CUSUM statistics. The average run sengths (ARLs) are computed for comparison with other charts particularly with some recently developed single charts. The SS-CUSUM chart has an advantage over its competitors since it quickly detects bot...

  11. Agaricus blazei Murill as an efficient hepatoprotective and antioxidant agent against CCl4-induced liver injury in rats

    OpenAIRE

    Al-Dbass, Abeer M.; Al- Daihan, Sooad K.; Bhat, Ramesa Shafi

    2012-01-01

    Agaricus blazei Murill is one of the very popular edible medicinal mushrooms. The present study investigated the protective effect of this biologically active mushroom on the tissue peroxidative damage and abnormal antioxidant levels in carbon tetrachloride induced hepatotoxicity in male albino rats. Male albino rats of Sprague–Dawley strain weighting (120–150 g) were categorized into five groups. The first group served as the normal control, the second and the third groups were treated with ...

  12. Protoplast Preparation of Jisongrong Mushroom (Agaricus blazei)%姬松茸原生质体的制备

    Institute of Scientific and Technical Information of China (English)

    叶勋艳; 张云

    2005-01-01

    对姬松茸(Agaricus blazei Murrill)原生质体制备的最佳条件进行了研究,结果表明:采用液体发酵培养第 7 d的菌体,用2%溶壁酶在 30 ℃下酶解 5 h,原生质体产率达9.08×10 6个/mL.

  13. Effects of an Agaricus blazei Aqueous Extract Pretreatment on Paracetamol-Induced Brain and Liver Injury in Rats

    Directory of Open Access Journals (Sweden)

    Andréia A. Soares

    2013-01-01

    Full Text Available The action of an Agaricus blazei aqueous extract pretreatment on paracetamol injury in rats was examined not only in terms of the classical indicators (e.g., levels of hepatic enzymes in the plasma but also in terms of functional and metabolic parameters (e.g., gluconeogenesis. Considering solely the classical indicators for tissue damage, the results can be regarded as an indication that the A. blazei extract is able to provide a reasonable degree of protection against the paracetamol injury in both the hepatic and brain tissues. The A. blazei pretreatment largely prevented the increased levels of hepatic enzymes in the plasma (ASP, ALT, LDH, and ALP and practically normalized the TBARS levels in both liver and brain tissues. With respect to the functional and metabolic parameters of the liver, however, the extract provided little or no protection. This includes morphological signs of inflammation and the especially important functional parameter gluconeogenesis, which was impaired by paracetamol. Considering these results and the long list of extracts and substances that are said to have hepatoprotective effects, it would be useful to incorporate evaluations of functional parameters into the experimental protocols of studies aiming to attribute or refute effective hepatoprotective actions to natural products.

  14. Serum hepatic biochemistry and electrophoretic protein profile of healthy and Ehrlich tumor-bearing mice treated with extracts of Agaricus blazei Murill

    Directory of Open Access Journals (Sweden)

    Durval Verçosa Junior

    2016-04-01

    Full Text Available Compounds isolated from Agaricus blazei Murill represent a group of promising natural immunomodulators for use in the treatment of neoplasms. We have evaluated the serum biochemical profile of healthy and Ehrlich tumor-bearing mice treated with different extracts of A. blazei. Total, supernatant, and polysaccharide extracts of A. blazei were obtained from suspensions (at acidic or neutral pH kept in a water bath at 60 °C or in an ultrasonic bath at 37 °C. After oral administering the extracts to mice for 21 days, blood samples were collected for determination of aspartate aminotransferase (AST, alanine aminotransferase (ALT, creatine kinase (CK, urea, total protein, albumin, globulins, and alpha-, beta- and gamma-globulin fractions. The presence of the tumor led to a significant increase in serum CK and AST activities and in the concentrations of total globulin and the gamma-globulin fraction, and to a decrease in the albumin and alpha2-globulin levels. The polysaccharide extracts of A. blazei reduced the serum AST and ALT activities, probably due to a hepatoprotective effect. In addition, polysaccharide and supernatant extracts inhibited the tumor-induced increase in gamma-globulin levels. Thus, the supernatant and polysaccharide fractions of the extract of A. blazei have potential for use in complementary antineoplastic treatments.

  15. Influence of Lentinus edodes and Agaricus blazei extracts on the prevention of oxidation and retention of tocopherols in soybean oil in an accelerated storage test.

    Science.gov (United States)

    da Silva, Ana Carolina; Jorge, Neuza

    2014-06-01

    This study aimed to evaluate the influence of the methanol extracts of mushrooms Lentinus edodes and Agaricus blazei on the retention of tocopherols in soybean oil, when subjected to an accelerated storage test. The following treatments were subjected to an accelerated storage test in an oven at 60 °C for 15 days: Control (soybean oil without antioxidants), TBHQ (soybean oil + 100 mg/kg of TBHQ), BHT (soybean oil + 100 mg/kg of BHT), L. edodes (soybean oil + 3,500 mg/kg of L. edodes extract) and A. blazei (soybean oil + 3,500 mg/kg of A. blazei extract). The samples were analyzed for tocopherols naturally present in soybean oil and mass gain. The results showed, the time required to reach a 0.5% increase in mass was 13 days for TBHQ and 15 days for A. blazei. The content of tocopherols for TBHQ was 457.50 mg/kg and the A. blazei, 477.20 mg/kg. PMID:24876658

  16. Avaliação in vitro do crescimento micelial de cinco linhagens de Agaricus blazei em duas temperaturas / In vitro evaluation of the mycelial growth of five Agaricus blazei strains in two temperatures

    Directory of Open Access Journals (Sweden)

    Diego Cunha Zied

    2011-04-01

    Full Text Available O objetivo do trabalho foi avaliar o crescimento micelial in vitro de cinco linhagens de Agaricus blazei (ABL-05/53, ABL-04/49, ABL-03/44, ABL-99/30 e ABL-02/51, quando submetidas às temperaturas de 20 e 25ºC. Em câmara de fluxo laminar, discos das linhagens foram inoculadas no centro de placas de Petri contendo o meio CA (composto-ágar e incubadas em estufa BOD. Após 48 horas, iniciaram-se as medidas do crescimento micelial, com auxílio de uma régua graduada em milímetros, através de quatro medições equidistantes entre si, até o momento em que, em um dos tratamentos, a colônia fúngica atingisse a proximidade das bordas da placa de Petri. O delineamento experimental foi inteiramente casualizado, em esquema fatorial 5 x 2. Cada tratamento constou de sete repetições, correspondente a uma placa de Petri, totalizando setenta unidades experimentais. Verificou-se que o crescimento de A. blazei é influenciado pela temperatura de incubação, sendo que a temperatura de 25°C foi mais favorável para o crescimento micelial de todas as linhagens de A. blazei avaliadas, com destaque para as linhagens ABL-04/49 e ABL-03/44 que obtiveram as maiores médias de crescimento micelial nesta condição de temperatura ao final do ciclo de cultivo.AbstractThe objective of the work was to evaluate the in vitro mycelial growth of five A. blazei strains (ABL-05/53, ABL-04/49, ABL-03/44, ABL-99/30 and ABL-02/51 when submitted to the temperatures of 20 and 25 ºC. In a laminar flow chamber, discs of the strains were inoculated in the middle of Petri’s plates containing CA (compost-agar medium and incubated in BOD. After 48 hours, measurements of the mycelial growth began, with the help of a ruler with scale in millimeters, by means of four equidistant measurements, until the moment when the fungal colony reached near the edges of the Petri’s plate in one of the treatments. The experimental design was totally randomized, in 5 x 2 factorial design

  17. Avaliação da coloração de basidiocarpos desidratados de Agaricus blazei segundo escala de Munsell Evaluation of the dehydrated fruiting bodies of the mushroom Agaricus blazei according to Munsell colour scale

    Directory of Open Access Journals (Sweden)

    Marisa Aparecida da Fonseca Delú

    2006-02-01

    Full Text Available Com este trabalho objetivou-se avaliar a coloração final de basidiocarpos desidratados de Agaricus blazei, após serem submetidos a tratamento com ácidos ascórbico e cítrico. Para tanto, utilizaram-se cogumelos A. blazei, cultivados em substrato apropriado e mantidos em estufa. Os cogumelos foram colhidos com o píleo totalmente fechado e após a limpeza em água corrente, os basidiocarpos foram seccionados ao meio no sentido longitudinal, sendo uma metade submetida ao tratamento experimental e a outra metade representando o tratamento controle. Os tratamentos experimentais consistiram da imersão das metades dos basidiocarpos em solução de ácido ascórbico comercial nas concentrações de 0, 10, 20 e 30 mg/L durante 0, 2, 5, 10, 15 ou 20 minutos. Para o ácido cítrico foram testadas as mesmas concentrações anteriores, porém nos tempos de imersão de 0, 10 e 15 minutos. Todos os tratamentos consistiram de 3 repetições cada. Não se observou relação entre os tratamentos utilizados com a coloração final dos basidiocarpos, embora a utilização de substâncias ácidas tenha possibilitado a obtenção de basidiocarpos desidratados mais próximos daquela coloração considerada ideal para comercialização.The main aim of this work was to evaluate the final colour of the fructification bodies of the mushroom Agaricus blazei submitted to ascorbic and citric acid treatment. The mushroom Agaricus blazei was cultivated in an appropriated substrate and storage in incubators. The mushroom was harvested containing closed pileus and after cleaning with running tap water, the fruiting bodies were sliced and half of each were submitted to acid treatment and half was the control (water immersion. The experimental treatment consisted of immersing half of each basiodiocarp in different concentrations of the commercial solution of ascorbic acid (0, 10, 20 and 30 mg/ml during 0, 2, 5, 10, 15 and 20 minutes. The other parts of fruiting bodies were

  18. The Medicinal Mushroom Agaricus blazei Murrill: Review of Literature and Pharmaco-Toxicological Problems

    Directory of Open Access Journals (Sweden)

    F. Firenzuoli

    2008-01-01

    Full Text Available Agaricus blazei Murrill (ABM popularly known as ‘Cogumelo do Sol’ in Brazil, or ‘Himematsutake’ in Japan, is a mushroom native to Brazil, and widely cultivated in Japan for its medicinal uses, so it is now considered as one of the most important edible and culinary-medicinal biotechnological species. It was traditionally used to treat many common diseases like atherosclerosis, hepatitis, hyperlipidemia, diabetes, dermatitis and cancer. In vitro and in vivo ABM has shown immunomodulatory and antimutagenic properties, although the biological pathways and chemical substances involved in its pharmacological activities are still not clear. The polysaccharides phytocomplex is thought to be responsible for its immunostimulant and antitumor properties, probably through an opsonizing biochemical pathway. Clinical studies are positive confirmations, but we are still at the beginning, and there are perplexing concerns especially relative to the content of agaritine. Argantine is a well-known carcinogenic and toxic substance in animals, that must be completely and fully evaluated.

  19. Phase I Clinical Study of the Dietary Supplement, Agaricus blazei Murill, in Cancer Patients in Remission

    Directory of Open Access Journals (Sweden)

    Satoshi Ohno

    2011-01-01

    Full Text Available Although many cancer patients use complementary and alternative medicine, including Agaricus blazei Murill (ABM, safety is not yet well understood. Cancer survivors took 1.8, 3.6, or 5.4 g ABM granulated powder (Kyowa Wellness Co., Ltd., Tokyo, Japan per day orally for 6 months. Adverse events were defined by subjective/objective symptoms and laboratory data according to the National Cancer Institute Common Terminology Criteria for Adverse Events version 3.0 (NCI-CTCAE v3.0. Seventy-eight patients were assessed for safety of ABM (30/24/24 subjects at 1/2/3 packs per day, resp.. Adverse events were observed in 9 patients (12%. Most were digestive in nature such as nausea and diarrhea, and one patient developed a liver dysfunction-related food allergy, drug lymphocyte product. However, none of these adverse events occurred in a dose-dependent manner. This study shows that ABM does not cause problems in most patients within laboratory parameters at the dosages tested over 6 months. This trial supports previous evidence that the ABM product is generally safe, excluding possible allergic reaction.

  20. An extract of the medicinal mushroom Agaricus blazei Murill can protect against allergy

    OpenAIRE

    Hetland Geir; Ellertsen Linda K

    2009-01-01

    Abstract Background Agaricus blazei Murill (AbM) is an edible Brazilian mushroom that has been used in traditional medicine for a range of diseases. It has been shown to have anti-infection and anti-tumor properties in the mouse, which are due to induction of Th1 responses. On the other hand, IgE-mediated allergy is induced by a Th2 response. Objective Since according to the Th1/Th2 paradigm an increased Th1 response may promote a reduced Th2 response, the aim was to examine whether AbM had a...

  1. Influência de Agaricus blazei Murrill sobre o tumor sólido de Ehrlich e linfonodos poplíteos de camundongos Influence of Agaricus blazei Murrill in solid Ehrlich tumor and popliteal lymph nodes in mice

    Directory of Open Access Journals (Sweden)

    D. Verçosa Junior

    2007-02-01

    Full Text Available Estudou-se a influência do tratamento diário com filtrado aquoso de Agaricus blazei Murrill (ABM (25mg/ml, via oral, por 17 e 57 dias, em camundongos inoculados com tumor sólido de Ehrlich (TSE por meio da curva de crescimento tumoral, do peso relativo do tumor, da contagem de regiões organizadoras de nucléolos (AgNORs e dos padrões histológicos das massas tumorais e linfonodos poplíteos. Os animais que ingeriram o extrato aquoso do ABM por 57 dias apresentaram menor (P0,05 entre os animais tratados e não tratados.The influence of daily administration of Agaricus blazei Murrill (ABM aqueous solution (25mg/ml during 17 and 57 days in mice bearing solid Ehrlich tumor was studied. Tumoral growth, tumoral and spleenic relative weights, nucleoly organization regions AgNORs values and tumor and popliteal lymph nodes histopathology were daily evaluated. The animals that received ABM during 57 days showed lower values (P0.05 between treated and non-treated animals. No difference in microscopic evaluation of the tumors in treated and non-treated animals was seen and metastasis in popliteal nodes of the tumor occurred in all the animals.

  2. Extratos de Lentinula edodes e Agaricus blazei sobre Bipolaris sorokiniana e Puccinia recondita f. sp. tritici, in vitro Extracts of Lentinula edodes and Agaricus blazei on Bipolaris sorokiniana and Puccinia recondita f. sp. tritici., in vitro

    Directory of Open Access Journals (Sweden)

    Ana Cristina Grade Fiori-Tutida

    2007-09-01

    Full Text Available Com o objetivo de buscar medidas alternativas para o controle de Bipolaris sorokiniana e Puccinia recondita f. sp. tritici testou-se o efeito fungitóxico in vitro dos cogumelos Lentinula edodes e Agaricus blazei sobre esses fungos. Os extratos brutos aquosos de ambos os cogumelos não tiveram efeito significativo tanto no crescimento micelial quanto na germinação de esporos de B. sorokiniana. Por outro lado, os extratos dos cogumelos inibiram a germinação de esporos de P. recondita f. sp. tritici, com destaque para o isolado LE 96/17 de L. edodes que apresentou inibição da ordem de 52,4%.With the purpose of finding alternative ways to control Bipolaris sorokiniana and Puccinia recondita f. sp. triciti, the fungitoxic effect in vitro of Lentinula edodes and Agaricus blazei mushrooms was tested on these fungi. The aqueous crude extract of both mushrooms did not have significant effect on mycelial growth and spore germination of B. sorokiniana . On the other hand, the mushroom extracts inhibited the germination of P. recondita f. sp. tritici uredospores, particularly on the L. edodes (96/17, which exhibited the largest inhibition of spore germination (52,38%.

  3. 23 July - Italian Director-General for Prevention G. Ruocco and Director-General for European and International Relations Ministry of Health D. Roderigo visiting the ATLAS experimental cavern with ATLAS Deputy Spokesperson B. Heinemann. Life Sciences Section M. Cirilli and Life Sciences Adviser M. Dosanjh present.

    CERN Multimedia

    Anna Pantelia

    2013-01-01

    23 July - Italian Director-General for Prevention G. Ruocco and Director-General for European and International Relations Ministry of Health D. Roderigo visiting the ATLAS experimental cavern with ATLAS Deputy Spokesperson B. Heinemann. Life Sciences Section M. Cirilli and Life Sciences Adviser M. Dosanjh present.

  4. 19 July 2013 - Chairman of the Policy Committee, European Cancer Organisation, President, European Association for Cancer Research E. Celis visiting the ATLAS experimental cavern with ATLAS Collaboration Deputy Spokesperson, B. Heinemann and signing the Guest Book with Director for Accelerators and Technology S. Myers. Life Sciences Adviser M. Dosanjh present.

    CERN Multimedia

    Anna Pantelia

    2013-01-01

    19 July 2013 - Chairman of the Policy Committee, European Cancer Organisation, President, European Association for Cancer Research E. Celis visiting the ATLAS experimental cavern with ATLAS Collaboration Deputy Spokesperson, B. Heinemann and signing the Guest Book with Director for Accelerators and Technology S. Myers. Life Sciences Adviser M. Dosanjh present.

  5. On the dopplars SS433

    International Nuclear Information System (INIS)

    A new family of X-ray sources has recently been proposed including SS433. The general relativistic formula to fit the frequency dependence of Hsub(α) lines in SS433 as a function of the phase is presented here. Particularly relevant for the verification of the model are the observations at the phase of minimum shift. (author)

  6. An extract of the medicinal mushroom Agaricus blazei Murill can protect against allergy

    Directory of Open Access Journals (Sweden)

    Hetland Geir

    2009-05-01

    Full Text Available Abstract Background Agaricus blazei Murill (AbM is an edible Brazilian mushroom that has been used in traditional medicine for a range of diseases. It has been shown to have anti-infection and anti-tumor properties in the mouse, which are due to induction of Th1 responses. On the other hand, IgE-mediated allergy is induced by a Th2 response. Objective Since according to the Th1/Th2 paradigm an increased Th1 response may promote a reduced Th2 response, the aim was to examine whether AbM had anti-allergy effects. Methods A mouse model for allergy was employed, in which the mice were immunized s.c. with the model allergen ovalbumin (OVA. Additionally, the animals were given a mushroom extract, AndoSan™, mainly (82% containing AbM, but also Hericium erinaceum (15% and Grifola frondosa (3%, or PBS p.o. either a day before or 19 days after the immunization. The mice were sacrificed on day 26, and anti-OVA IgE (Th2 response and IgG2a (Th1 response antibodies were examined in serum and Th1, Th2 and Treg cytokines in spleen cells cultures. Results It was found that the AndoSan™ extract both when given either before or after OVA immunization reduced the levels of anti-OVA IgE, but not IgG2a, in the mice. There was a tendency to reduced Th2 relative to Th1 cytokine levels in the AndoSan™ groups. Conclusion This particular AbM extract may both prevent allergy development and be used as a therapeutical substance against established allergy.

  7. The Mushroom Agaricus blazei Murill Elicits Medicinal Effects on Tumor, Infection, Allergy, and Inflammation through Its Modulation of Innate Immunity and Amelioration of Th1/Th2 Imbalance and Inflammation

    OpenAIRE

    Geir Hetland; Egil Johnson; Torstein Lyberg; Gunnar Kvalheim

    2011-01-01

    The medicinal mushroom Agaricus blazei Murill from the Brazilian rain forest has been used in traditional medicine and as health food for the prevention of a range of diseases, including infection, allergy, and cancer. Other scientists and we have examined whether there is scientific evidence behind such postulations. Agaricus blazei M is rich in the immunomodulating polysaccharides, β -glucans, and has been shown to have antitumor, anti-infection, and antiallergic/-asthmatic properties in mo...

  8. Antimutagenic action of Lentinula edodes and Agaricus blazei on Aspergillus nidulans conidia Ação antimutagênica de Lentinula edodes e Agaricus blazei em conídios de Aspergillus nidulans

    Directory of Open Access Journals (Sweden)

    Edneia A. Souza-Paccola

    2004-12-01

    Full Text Available The antimutagenic effect of the mushrooms Lentinula edodes and Agaricus blazei was studied on conidia of Aspergillus nidulans when exposed to short wave ultraviolet light. Two strains of A. nidulans were used. For the preparation of the extracts, the fresh mushrooms were left in aqueous infusion for 12 hours and heated in a water bath for 15 min at 100ºC, and then the material was filtered. The dehydrated mushrooms were left in aqueous infusion for 12 hours and to filtrated. Both filtrates were used as extracts. A. nidulans conidia were incubated for three hours in water and in mushroom extracts and only after were exposed to UV light (pre-treatment. A. nidulans conidia were suspended in water and in mushroom extracts and immediately submitted to UV light (post-treatment. Conidial suspension in water and in mushroom extracts but without exposure to the mutagenic agent were used as controls. After mutagenic treatment, it was observed an increase in the survival rate of the A. nidulans and a decrease in the percentage of morphologic mutants on conidia treated with mushroom extracts. Our results demonstrated the radioprotective and antimutagenic effect of L. edodes and A. blazei mushrooms on eukaryotic cells when exposed to UV radiation.O efeito antimutagênico dos cogumelos Lentinula edodes e Agaricus blazei foram estudados sobre conídios de Aspergillus nidulans quando expostos à luz ultravioleta de comprimento de onda curto. Duas linhagens de A. nidulans foram usadas. Para o preparo dos extratos, os cogumelos frescos permaneceram em infusão aquosa por 12 horas e em seguida foram aquecidos em banho-maria por 15 min à 100ºC e a seguir o material foi filtrado. Os cogumelos desidratados foram deixados em infusão aquosa por 12 horas e a seguir filtrados. Ambos os filtrados foram usados como extratos. Os conídios de A. nidulans foram incubados por três horas em água e em extrato de cogumelo e somente após foram expostos a luz ultravioleta

  9. Common desease and insect in Agaricus blazei and their control%巴西蘑菇常见病虫害及防治

    Institute of Scientific and Technical Information of China (English)

    林衍铨; 黄建成; 胡润芳

    2000-01-01

    @@ 巴西蘑菇(Agaricus blazei Murrill,日本商品名称姬松茸)在栽培过程中,往往会出现各种病、虫害,轻者降低品质,造成减产,重者甚至绝收.现将巴西蘑菇常见的病虫害及其防治措施介绍如下.

  10. 巴西蘑菇Agaricus blazei Murill水溶性多糖部分理化性质的研究%Some Properties of Water - soluble Polysaccharides from Agaricus blazei Murill Fruit Body

    Institute of Scientific and Technical Information of China (English)

    林跃鑫; 叶竹秋; 黄谚谚; 谢华玲

    2002-01-01

    本文以物理及化学方法研究了巴西蘑菇Agaricus blazei Murill水溶性多糖AB-Ⅰ、AB-Ⅱ-b和AB-Ⅲ-b的理化性质,结果表明,3种经纯化的多糖都不含糖醛酸而含蛋白质,蛋白质含量分别为AB-Ⅰ:20.1%、AB-Ⅱ-b:16.3%、AB-Ⅲ-b:25.6%.它们的比旋度分别为AB-Ⅰ:+44.33°、AB-Ⅱ-b;+3.70°、AB-Ⅲ-b:+5.04°.AB-Ⅰ、AB-Ⅱ-b的1.0%水溶液均具有良好的增稠性,其粘度具有很好的抗盐浓度、耐热和对广泛pH的稳定性能.

  11. Aqueous Extract of Agaricus blazei Murrill Prevents Age-Related Changes in the Myenteric Plexus of the Jejunum in Rats

    Directory of Open Access Journals (Sweden)

    Ana Paula de Santi-Rampazzo

    2015-01-01

    Full Text Available This study evaluated the effects of the supplementation with aqueous extract of Agaricus blazei Murrill (ABM on biometric and blood parameters and quantitative morphology of the myenteric plexus and jejunal wall in aging Wistar rats. The animals were euthanized at 7 (C7, 12 (C12 and CA12, and 23 months of age (C23 and CA23. The CA12 and CA23 groups received a daily dose of ABM extract (26 mg/animal via gavage, beginning at 7 months of age. A reduction in food intake was observed with aging, with increases in the Lee index, retroperitoneal fat, intestinal length, and levels of total cholesterol and total proteins. Aging led to a reduction of the total wall thickness, mucosa tunic, villus height, crypt depth, and number of goblet cells. In the myenteric plexus, aging quantitatively decreased the population of HuC/D+ neuronal and S100+ glial cells, with maintenance of the nNOS+ nitrergic subpopulation and increase in the cell body area of these populations. Supplementation with the ABM extract preserved the myenteric plexus in old animals, in which no differences were detected in the density and cell body profile of neurons and glial cells in the CA12 and CA23 groups, compared with C7 group. The supplementation with the aqueous extract of ABM efficiently maintained myenteric plexus homeostasis, which positively influenced the physiology and prevented the death of the neurons and glial cells.

  12. 姬松茸液体培养基的筛选%The Selection on Culture Medium of Agaricus blazei Murrill

    Institute of Scientific and Technical Information of China (English)

    周选围

    2001-01-01

    Agaricus blazei Murrill is one of the edible and m edicinal fungi.In this paper,through the selection and study of the improted Agaricus blazei Murrill in submerged culture medium,the experimental r esults show that the most suitable composition of the Agaricus blazei Murrill submerged culture medium is as follow:3.0% corn powder,0.3% yeast conc e ntration,2.0% sugar,0.05%(NH4)2SO4,0.1%MgSO4,0.1%KH2PO4.In thi s liquid medium,the mycelium grows vigorous,it gets to 539mg/100mL(DW)for 8 d ays.%对引进的姬松茸菌种进行了液体培养基的筛选研究,认为姬松茸液体培养最适合的培养基配方为:玉米粉3.0%,酵母提取物0.3%,蔗糖2.0%,硫酸铵0 .05%,硫酸镁0.1%,磷酸二氢钾0.1%。在这种培养基中,姬松茸生长旺盛,产生的菌丝球数量多,颜色白,大小均一,8d后生物量达53.9%mg/L(DW)。

  13. 姬松茸颗粒剂扶正作用的实验研究%Researching on Experimentation in strengthening body resistance of Agaricus blazei Muriu

    Institute of Scientific and Technical Information of China (English)

    王伟明; 张云凌

    2004-01-01

    姬松茸(Agaricus blazei Muriu)又称巴西菇,是一种食、药两用真菌,姬松茸富含多种丰富的营养成分。姬松茸颗粒剂系采用新工艺从姬松茸中提取的主要成分为粗多糖的一种制剂,我们对其抗肿瘤和对免疫功能的影响等方面进行了实验研究,现报道如下。

  14. 工厂化栽培姬松茸的关键技术%Factory Cultivation Key Techniques of Agaricus blazei

    Institute of Scientific and Technical Information of China (English)

    曾先富; 熊维全; 李昕竺; 刘洪

    2015-01-01

    姬松茸(Agaricus blazei)是一种珍稀食(药)用菌.对姬松茸工厂化栽培技术中菌种选择与制作、培养料配方与发酵、铺料与播种、菌丝体培养、覆土、出菇管理、采收与转潮管理、病虫害绿色防控等关键技术点进行了介绍.

  15. Studies on Odontotermes formosanus Food Choice between Agaricus blazei and Its Substrate%黑翅土白蚁对姬松茸及其培养料的取食性研究

    Institute of Scientific and Technical Information of China (English)

    苏德伟; 林辉; 罗海凌; 林春梅; 林占熺

    2012-01-01

    In order to evaluate the impact of Odontotermes formosanus to Agaricus blazei Murrill cultivation, studies were carried out on food selectiveness of Odontotermes formosanus by observing 5 different materials ( fruitbody of Agaricus blazei Murrill, bamboo powder, Juncao, Juncao substrate of Agaricus blazei Murrill, bamboo substrate of Agaricus blazei Murrill) foraging behaviors. The results showed that consumption rate of Juncao and bamboo powders were very significantly higher than that of other 3 materials. And the consumption rate of Agaricus blazei Murrill was 0. The mud sheet area and mud sheet coverage rate of Juncao and bamboo powders were very significantly higher than that of the other 3 materials. These all indicated that the volatile substances distributed from mycelium and fruitbody of Agaricus blazei Murrill could inhibit termites' feeding. And the harms of termites in Agaricus blazei Murrill were mainly from cultivation materials.%通过观察黑翅土白蚁工蚁的觅食行为,进行了黑翅土白蚁对5种材料(姬松茸、木屑、菌草草粉、菌草姬松茸培养料、木屑姬松茸培养料)的取食选择性研究.结果表明,木屑和菌草草粉的被食率极显著高于其他3种材料,而姬松茸被食率为0;木屑与菌草草粉的泥被面积和泥被覆盖率极显著高于其他3种材料.这都表明姬松茸菌丝及其子实体散发出来的挥发性物质可以抑制黑翅土白蚁取食,黑翅土白蚁为害姬松茸主要是对其栽培料的破坏.

  16. Spectral Components of SS 433

    CERN Document Server

    Gies, D R; Riddle, R L; Wang, Z; Wiita, P J; Wingert, D W

    2002-01-01

    We present results from new optical and UV spectroscopy of the unusual binary system SS 433, and we discuss the relationship of the particular spectral components we observe to the properties of the binary. (1) The continuum spectrum which we associate with flux from the super-Eddington accretion disk and the dense part of its wind. (2) H-alpha moving components which are formed far from the binary orbital plane in the relativistic jets. (3) H-alpha and He I "stationary" emission lines which we suggest are formed in the disk wind in a volume larger than the dimensions of the binary. (4) A weak "stationary" emission feature we identify as a C II 7231,7236 blend that attains maximum radial velocity at the orbital quadrature of disk recession. (5) Absorption and emission features from outflowing clumps in the disk wind (seen most clearly in an episode of blue-shifted Na I emission). (6) We found no clear evidence of the absorption line spectrum of the optical star, although we point out the presence of He I abso...

  17. 28 November 2013 - N. N. Kudryavtsev, Russian Rector of the Moscow Institute of Physics and Technology signing an Agreement and the Guest Book with CERN Director-General R. Heuer; visiting the ATLAS cavern with ATLAS Deputy Spokesperson B. Heinemann and visiting the LHC tunnel at Point 1 with AGH University of Science and Technology A. Erokhin. M. Savino, Physics Department, Joint Institute for Nuclear Research also present.

    CERN Multimedia

    Jean-Claude Gadmer

    2013-01-01

    28 November 2013 - N. N. Kudryavtsev, Russian Rector of the Moscow Institute of Physics and Technology signing an Agreement and the Guest Book with CERN Director-General R. Heuer; visiting the ATLAS cavern with ATLAS Deputy Spokesperson B. Heinemann and visiting the LHC tunnel at Point 1 with AGH University of Science and Technology A. Erokhin. M. Savino, Physics Department, Joint Institute for Nuclear Research also present.

  18. Primary mechanism of apoptosis induction in a leukemia cell line by fraction FA-2-b-ß prepared from the mushroom Agaricus blazei Murill

    Directory of Open Access Journals (Sweden)

    L. Gao

    2007-11-01

    Full Text Available Agaricus blazei Murill is a native Brazilian mushroom which functions primarily as an anticancer substance in transplanted mouse tumors. However, the mechanism underlying this function of A. blazei Murill remains obscure. The present study was carried out to investigate the effect of fraction FA-2-b-ß, an RNA-protein complex isolated from A. blazei Murill, on human leukemia HL-60 cells in vitro. Typical apoptotic characteristics were determined by morphological methods using DNA agarose gel electrophoresis and flow cytometry. The growth suppressive effect of fraction FA-2-b-ß on HL-60 cells in vitro occurred in a dose- (5-80 µg/mL and time-dependent (24-96 h manner. The proliferation of HL-60 cells (1 x 10(5 cells/mL treated with 40 µg/mL of fraction FA-2-b-ß for 24-96 h and with 5-80 µg/mL for 96 h resulted in inhibitory rates ranging from 8 to 54.5%, and from 4.9 to 86.3%, respectively. Both telomerase activity determined by TRAP-ELISA and mRNA expression of the caspase-3 gene detected by RT-PCR were increased in HL-60 cells during fraction FA-2-b-ß treatment. The rate of apoptosis correlated negatively with the decrease of telomerase activity (r = 0.926, P < 0.05, but correlated positively with caspase-3 mRNA expression (r = 0.926, P < 0.05. These data show that fraction FA-2-b-ß can induce HL-60 cell apoptosis and that the combined effect of down-regulation of telomerase activity and up-regulation of mRNA expression of the caspase-3 gene could be the primary mechanism of induction of apoptosis. These findings provide strong evidence that fraction FA-2-b-ß could be of interest for the clinical treatment of acute leukemia.

  19. Use of Agaricus blazei and Lentinula edodes extracts for post-harvest control of gray mold in ‘Itália’ grape Extracto de Agaricus blazei y Lentinula edodes en el control de podredumbre gris en uva ‘Itália’ Extrato de Agaricus blazei e Lentinula edodes no controle pós-colheita de mofo cinzento em uva ‘Itália’

    Directory of Open Access Journals (Sweden)

    ELISANGELA CLARETE CAMILI

    2010-04-01

    Full Text Available

    Na proteção de uva ‘Itália’ pós-colheita contra Botrytis cinerea avaliou-se in vivo o efeito direto e indireto dos extratos aquosos dos cogumelos Agaricus blazei e Lentinula edodes através do tratamento dos cachos antes ou após a inoculação com o patógeno. Para tanto se realizaram dois ensaios: 1 cachos de uva foram inoculados e, após 4 h, aspergidos com diferentes concentrações dos extratos dos basidiocarpos (0,0; 2,5; 5,0; 10,0; 20,0 ou 40,0%; 2 cachos foram, ou não, aspergidos com extrato de A. blazei (5,0% 24, 48, 72 ou 96 h antes da inoculação do fungo. Para inoculação, em cada cacho foram feridas 10 bagas, fazendo-se um furo por baga de ±2 mm de profundidade, procedendo-se em seguida, a aspersão da suspensão de conídios (±105 conídios mL-1. Após os tratamentos, os cachos foram mantidos a 25±1 ºC/80-90% UR e avaliados diariamente quanto à incidência e severidade da podridão. O efeito in vitro dos extratos de A. blazei e L. edodes no controle do patógeno foi avaliado com o intuito de verificar se tais agentes exercem efeito direto sobre o crescimento micelial e a germinação de conídios de B. cinerea. Os resultados mostraram que os extratos de A. blazei e L. edodes não controlaram a podridão causada por B. cinerea em uva ‘Itália’, quando aplicados antes ou após a inoculação do fungo. Nos ensaios in vitro, ambos os extratos dos basidiocarpos estimularam a germinação dos conídios de B. cinerea nas concentrações testadas; quanto ao crescimento micelial, L. edodes retardou, enquanto A. blazei estimulou.

    For protecting ‘Itália’ against Botrytis cinerea in the post harvest it was evaluated in vivo direct and indirect effect of water based extracts of Agaricus blazei and Lentinula edodes

  20. The Mushroom Agaricus blazei Murill Elicits Medicinal Effects on Tumor, Infection, Allergy, and Inflammation through Its Modulation of Innate Immunity and Amelioration of Th1/Th2 Imbalance and Inflammation

    Directory of Open Access Journals (Sweden)

    Geir Hetland

    2011-01-01

    Full Text Available The medicinal mushroom Agaricus blazei Murill from the Brazilian rain forest has been used in traditional medicine and as health food for the prevention of a range of diseases, including infection, allergy, and cancer. Other scientists and we have examined whether there is scientific evidence behind such postulations. Agaricus blazei M is rich in the immunomodulating polysaccharides, β-glucans, and has been shown to have antitumor, anti-infection, and antiallergic/-asthmatic properties in mouse models, in addition to anti-inflammatory effects in inflammatory bowel disease patients. These effects are mediated through the mushroom's stimulation of innate immune cells, such as monocytes, NK cells, and dendritic cells, and the amelioration of a skewed Th1/Th2 balance and inflammation.

  1. 姬松茸母种培养基筛选研究初报%A Preliminary Study on the Selection of Mother Culture Media of Agaricus blazei Mürrill

    Institute of Scientific and Technical Information of China (English)

    陈文强; 周选围; 邓百万

    2002-01-01

    对姬松茸(Agaricus blazei Mtirrill)进行了母种培养基筛选试验.结果表明,姬松茸母种的最适培养基为:蔗糖25g,玉米淀粉30g,磷酸二氢钾3g,硫酸铵3g,维生素B1 10mg,琼脂20g.

  2. Research on the technology of composting sugarcane leaf in a fermentation tunnel for Agaricus blazei murill. cultivation%姬松茸蔗叶培养料隧道式发酵技术研究

    Institute of Scientific and Technical Information of China (English)

    谢毅栋; 覃喜强; 黄华

    2015-01-01

    通过对比糖料蔗叶和稻草为主原料,以隧道发酵和常规堆沤两种发酵方式制备栽培姬松茸的培养料研究,形成培养料制备过程中温度变化曲线。结果表明,以蔗叶为主原料栽培姬松茸完全可行,隧道发酵比常规堆沤时间大幅缩短,培养料质量稳定,能大幅提高姬松茸产量。%Through studying the research results for two different methods (fermentation tunnel and regular fermentation) of cultivating composting sugarcane leaf and straw for Agaricus blazei murill. plantation, as well as the temperature change records during the production of the compost, the experiment proves that it is doable to utilize sugarcane based compost to cultivate Agaricus blazei murill. Fermentation in a tunnel is dramatically faster than the regular fermentation method. The quality of the compost is stable and can increase the production of the Agaricus blazei murill.

  3. 47 CFR 97.311 - SS emission types.

    Science.gov (United States)

    2010-10-01

    ... 47 Telecommunication 5 2010-10-01 2010-10-01 false SS emission types. 97.311 Section 97.311... SERVICE Technical Standards § 97.311 SS emission types. (a) SS emission transmissions by an amateur... station in another country that permits such communications. SS emission transmissions must not be...

  4. Muutosjohtaminen Lindorff Oy.ssä

    OpenAIRE

    Huuskonen, Ville

    2011-01-01

    Muutos on pysyvä tila nykyajan liiketoiminnassa. Kehittynyt teknologia, vaativammat asiakkaat ja kovempi kilpailu ovat tekijöitä, joiden mukaan yritysten tulee pystyä kehittämään toimintaansa. Yritysten toimintaympäristö muuttuu jatkuvasti ja paikallaan pysyvät stabiilit yritykset eivät enää menesty kilpailussa. Muutos tuo mahdollisuuksien lisäksi myös epävarmuutta. Onnistunut muutosprosessi vaatii aina hyvää johtamista. Tässä opinnäytetyössä tarkastellaan muutosjohtamisen haasteita...

  5. Huumori toimintaterapeutin työssä

    OpenAIRE

    Liinamo, Liisa

    2006-01-01

    Koko teksti, Tiivistelmä, Abstract Tutkimuksen tarkoituksena oli kuvata huumoria toimintaterapeutin työssä. Tutkimuksen aineistona oli neljän toimintaterapeutin haastattelut, joissa pohdittiin, mitä huumori oli, ilmenikö sitä toimintaterapiassa, miten sitä ilmeni ja mitä merkitystä huumorilla oli toimintaterapeutin työssään. Haastatteluaineistoon perehdyttiin käyttäen aineistolähtöistä sisällön analyysiä. Teoriaosuudessa huumoria tarkasteltiin eri tieteenalojen- ja toimintaterapiakirjallis...

  6. Middle Level SS&C Energy Series.

    Science.gov (United States)

    Crow, Linda W.; Aldridge, Bill G.

    The project on Scope Sequence and Coordination of Secondary School Science (SS&C) was initiated by the National Science Teachers Association (NSTA) and recommends that all students study science every year and advocates carefully sequenced, well-coordinated instruction in biology, chemistry, earth/space science, and physics. This document…

  7. Asiantuntijapalvelun tuotteistaminen : yhteistyössä vastuullisuutta edistämässä

    OpenAIRE

    Asteljoki, Kaj

    2010-01-01

    Työssä on liitetty yhteen vastuullinen liiketoiminta ja strateginen johtaminen kestävän kehityksen näkökulmasta, tavoitteena asiakasyrityksen pitkän aikavälin menestyminen. Vastuullinen liiketoiminta on organisaatiolle strateginen valinta, jonka sudenkuoppana on käytäntöön vieminen. Tämä sama ongelma on yleinen strategisessa johtamisessa. Tässä työssä kehitetään vastuullisen liiketoimintastrategian laatimiseen ja käytäntöön viemiseen soveltuvia yhteistoiminnallisia, osallistavia menettelyjä. ...

  8. Indução de resistência em tomateiro por extratos aquosos de Lentinula edodes e Agaricus blazei contra Ralstonia solanacearum Induction of resistance in tomato plants by aqueous extracts of Lentinula edodes and Agaricus blazei against Ralstonia solanacearum

    Directory of Open Access Journals (Sweden)

    Ricardo F. Silva

    2007-06-01

    Full Text Available O presente trabalho foi conduzido com o objetivo de avaliar o efeito dos extratos aquosos dos cogumelos Lentinula edodes e Agaricus blazei e do indutor de resistência acibenzolar-S-metil (ASM no controle da murcha bacteriana em tomate (Lycopersicon esculentum Mill., bem como investigar o modo de ação destes produtos na atividade protetora, através de alterações na atividade de determinadas enzimas. Extratos aquosos dos cogumelos e o ASM foram testados na inibição do crescimento da bactéria in vitro. Em casa-de-vegetação, plantas foram tratadas com diferentes doses dos extratos e com ASM (0,05 g/L, dois dias antes da inoculação. As avaliações foram efetuadas com base na severidade da doença e determinação da atividade de algumas enzimas. Os isolados de A. blazei e L. edodes, bem como o ASM, não exerceram efeito inibitório direto no crescimento da bactéria. Quanto à indução de resistência, o isolado Le-96/17 de L. edodes na concentração de 10 % (v/v e o ASM reduziram significativamente a ocorrência de murcha. As atividades de quitinase, fenilalanina amônia-liase e polifenoloxidase não foram alteradas para a maioria dos tratamentos, porém foi constatado um aumento na atividade de quitinase nas plantas tratadas com ASM. Para a peroxidase, os tratamentos ASM, Abl-26 e Le-96/17 ocasionaram aumentos na atividade da enzima e, com base nos resultados, o cogumelo L. edodes e o ASM apresentam potencial para reduzir a murcha bacteriana provavelmente através da indução de resistência.This work deals with the effect of aqueous extracts from the mushrooms Lentinula edodes and Agaricus blazei and the inducer of resistance acibenzolar-S-methyl (ASM on the control of bacterial wilt in tomato (Lycopersicon esculentum Mill., as well as investigating changes in enzyme activities. Aqueous extracts of the mushrooms and ASM were tested for inhibition of bacterial growth in vitro. Plants were treated with different concentrations of

  9. SS433: The microquasar link with ulxs?

    Directory of Open Access Journals (Sweden)

    P. A. Charles

    2004-01-01

    Full Text Available SS433 es el microcuasar prototipo de la Galaxia y posiblemente incluso an alogo a las fuentes ULX si se tiene en cuenta la energ a cin etica de los \\jets". Sin embargo y a pesar de 20 a~nos de estudio, nuestras restricciones sobre la naturaleza del sistema binario son extremadamente grandes debido a la di cultad de localizar rasgos espectrales capaces de revelar la naturaleza y el movimiento del donador de la masa. Los espectros azules de alta resoluci on recientemente obtenidos cuando el disco (en precesi on se encuentra de per l, sugieren que la binaria se halla cerca de la fase de envolvente com un, lo cual hace extremadamente dif cil obtener las restricciones cin eticas. Sin embargo, encontramos evidencia de un donador masivo como era de esperar para la muy alta tasa de transferencia de masa inferida y comparamos las propiedades de SS433 con las de Cyg X3.

  10. Toivo psykiatrisessa hoitotyössä

    OpenAIRE

    Jokela, Ville; Moberg, Hanna

    2016-01-01

    Opinnäytetyön tarkoituksena oli selvittää hoitajien näkökulmasta potilaan toivon ilmenemistä ja tukemista psykiatrisessa hoitotyössä. Opinnäytetyön aihe tuli Pirkanmaan sairaanhoitopiiriltä. Opinnäytetyön tehtävinä oli selvittää, miten toivo ilmenee psykiatrisessa hoitotyössä, mikä on hoitajan merkitys potilaan toivon kannalta, millä käytännön hoitotyön keinoilla potilaan toivoa voidaan tukea, mikä merkitys sosiaalisilla suhteilla on potilaan toivolle sekä miten toivottomuus ilmenee psykiatri...

  11. SS-IDS: Statistical Signature Based IDS

    OpenAIRE

    Gupta, Payas; Raïssi, Chedy; Dray, Gérard; Poncelet, Pascal; Brissaud, Johan

    2009-01-01

    Security of web servers has become a sensitive subject today. Prediction of normal and abnormal request is problematic due to large number of false alarms in many anomaly based Intrusion Detection Systems(IDS). SS-IDS derives automatical ly the parameter profiles from the analyzed data thereby generating the Statistical Signatures. Statistical Signatures are based on modeling of normal requests and their distribution value without explicit intervention. Several attributes are used to calculate...

  12. Blood parameters of sheep with high infection of Haemonchus contortus and treated with “mushroom of the sun” (Agaricus blazei

    Directory of Open Access Journals (Sweden)

    Gabriela Almeida Bastos

    2016-04-01

    Full Text Available Blood and parasitological parameters of sheep experimentally infected with Haemonchus contortus and treated with “mushroom of the sun” (Agaricus blazei were assessed. Lambs infected with the nematode were divided into three groups: treated with basidiocarp powder from the fungus, wormed with trichlorfon, and a control group that did not receive anthelmintic. Split-plot design analysis was performed where the treatments were defined as plots and four periods of collection were defined as subplots. Interaction between the treatments and the collection periods for the fecal egg counts per gram of feces (FEC was observed. Treatment with “mushroom of the sun” showed anthelmintic efficacies ranging from 28.6 to 54.2%. Similar performances to weight gain were observed among the groups. A significant interaction between treatments and evaluation periods was observed for erythrocyte counts, hematocrit value, and serum concentrations of albumin and urea. The values of the mean corpuscular volume, erythrocyte distribution width, and leukocyte, neutrophil, lymphocyte, monocyte, and eosinophil counts, as well as the mean platelet volume, varied among the collection days. The administration of “mushroom of the sun” significantly reduced the FEC, did not influence on the erythrocyte count and hematocrit value, which remained within normal limits. However it increased the serum concentrations of albumin.

  13. Oral Treatment with Extract of Agaricus blazei Murill Enhanced Th1 Response through Intestinal Epithelial Cells and Suppressed OVA-Sensitized Allergy in Mice

    Directory of Open Access Journals (Sweden)

    Go Bouike

    2011-01-01

    Full Text Available To clarify the mechanism of the antiallergic activity of Agaricus blazei Murill extract (ABME, the present paper used an in vivo allergy model and an in vitro intestinal gut model. During OVA sensitization, the serum IgE levels decreased significantly in ABME group. Interleukin (IL-4 and -5 produced from OVA-restimulated splenocytes was significantly decreased, and anti-CD3ε/CD28 antibody treatment also reduced IL-10, -4, and -5 production and increased IFN-γ production in ABME group. These results suggest that oral administration of ABME improves Th1/Th2 balance. Moreover, a coculture system constructed of Caco-2 cells and splenocytes from OT-II mice or RAW 264.7 cells indicated that the significant increases in IFN-γ production by ABME treatment. Therefore, it was concluded that the antiallergic activity of ABME was due to the activation of macrophages by epithelial cells and the promotion of the differentiation of naïve T cells into Th1 cells in the immune.

  14. SS wireless modem for an automatic vending machine; Jido hanbaikiyo SS musen modemu

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2000-01-10

    Low-cost network communication systems are required when introducing an information collecting system and an electronic money system for automatic vending machines. Development was made recently on a spread spectrum (SS) wireless modem capable of structuring a network incurring no running cost for wiring works and communications. The modem is intended of being used at locations where automatic vending machines are installed at relatively high concentration, such as railway stations, buildings and car parking areas. Its major features are as follows: (1) packet transfer is performed while data are relayed at high speed between arbitrary SS wireless modems; (2) high reliability communication is possible because an optimal relaying route is selected even at line impediments or failure of the SS wireless modems; and (3) the modem can be applied even to large-scale systems. (translated by NEDO)

  15. Vaikuttamisen keinot yritysten vastuuviestinnässä

    OpenAIRE

    Heikura, Jenni

    2016-01-01

    ssä opinnäytetyössä käsiteltiin yritysten vastuuviestintää. Muodoltaan työ oli tutkimustyyppinen opinnäytetyö, jonka tarkoituksena oli tutkia vaikuttavuuden keinoja yritysten vastuuviestinnässä. Lisäksi työssä pohdittiin vastuuviestinnän keskeisiä ongelmia ja luotettavuutta. Opinnäytetyössä tarkasteltiin kahden yrityksen vastuuviestintää. Yrityksiksi valikoitiin UPM-Kymmene Oyj ja Stora Enso Oyj. Tutkimuksen aineistona käytettiin kyseisten yritysten vastuuviestintää niiden omilla intern...

  16. Potilasturvallisuus perioperatiivisessa hoitotyössä -systemaattinen kirjallisuuskatsaus

    OpenAIRE

    Hartikainen, Saara

    2012-01-01

    ssä opinnäytetyössä tavoitteena oli selvittää, mitä on potilasturvallisuus perioperatiivisessa hoitotyössä. Huomioon otettiin sekä potilaan fyysinen että psyykkinen turvallisuus. Potilasturvallisuutta tarkasteltiin pre-, intra-, ja postoperatiivisen hoitotyön vaiheissa. Tässä opinnäytetyössä postoperatiivisen turvallisuuden tarkastelu päättyi heräämöön. Opinnäytetyö toteutettiin systemaattisena kirjallisuuskatsauksena. Lähdeaineiston keruu suoritettiin maaliskuussa 2012 käyttäen Saima...

  17. Aistit matkailun markkinointiviestinnässä

    OpenAIRE

    Paavola, Anni

    2010-01-01

    Opinnäytetyö oli alun perin osa Mmm..(Moniaistisuus Matkailun markkinointiviestinnässä)-hanketta, joka oli Laurea-ammattikorkeakoulun ja Valtion teknillisen tutkimuskeskuksen (VTT) rinnakkaishanke. Hankkeessa oli useita eri yhteistyökumppaneita, esimerkiksi Eckerö Line, Lasten Päivän Säätiö ja Metsähallitus. Hankkeen tavoitteena oli tutkia moniaistisuutta matkailuyritysten tuotteissa ja palveluissa. Hankkeen toinen tavoite oli ymmärryksen lisääminen eri aistien hyödyistä, vaikutuksista ja käy...

  18. Palveluinnovaatioita etsimässä

    OpenAIRE

    Toivanen, Sanna

    2014-01-01

    Opinnäytetyössä tutkitaan palveluinnovaatio- ja palvelumuotoilu-menetelmien roolia uusien liiketoimintaideoiden etsimisessä. Tutkimuksessa halutaan osoittaa, että uusia innovatiivisia ideoita voidaan löytää käyttäjien kokemuksiin tutustumalla ja yhteissuunnittelulla käyttäjien kanssa. Tutkimuksen toinen tavoite on toteuttaa tutkimus siten, että se voidaan toistaa toisella samankaltaisella korkean intensiteetin tuotealueella. Tutkimuksessa on teoriaosuus sekä käytännön osuus. Teoriaosuus k...

  19. Toimintaterapia lastensuojelun perhetyössä

    OpenAIRE

    Myllykoski, Heli; Mertanen, Mira; Ollila, Kirsi

    2012-01-01

    Tämä opinnäytetyömme perustuu tutkimukselliseen kehittämistyöhön, joka toteutettiin yhteistyössä Merikratos Lastensuojelu Oy:n perhetyön kanssa. Merikratos Lastensuojelu Oy on yksityinen sosiaalialan yritys, joka tarjoaa kunnille ostopalveluna lastensuojelun avohuollon ja sijaishuollon tukitoimia. Perhetyö on lastensuojelun avohuollon tukitoimi perheille, joista on herännyt lastensuojelullinen huoli. Lastensuojelun palveluiden tulisi pystyä vastaamaan entistä paremmin ja tehokkaammin lasten j...

  20. Empatiataitoa etsimässä

    OpenAIRE

    Erkamo, Merja; Ulkuniemi, Maria

    2013-01-01

    Tutkimuksella pyrittiin selvittämään minkälaisia keinoja varhaiskasvattajilla on päivähoidossa käytössä empatiataitojen tukemiseksi. Tutkimuksella kartoitettiin myös varhaiskasvattajien näkemyksiä siitä, olisiko empatiataitojen tukemiselle päivähoidossa kehittämistarvetta. Tutkimuksessa käytettiin tausta- ja tulkintateoriaa. Taustateoria käsittelee lapsen kehitystä ja empatian kehitystä. Tulkintateoriaa käytettiin tutkimusosiossa ja analysoinnin apuna. Tutkimus perustuu laadulliseen tutk...

  1. MULTIWAVELENGTH OBSERVATIONS OF THE SS 433 JETS

    OpenAIRE

    Marshall, Herman L.; Canizares, Claude R.; Hillwig, Todd; Mioduszewski, Amy; Rupen, Michael; Schulz, Norbert S.; Nowak, Michael; Heinz, Sebastian

    2013-01-01

    We present observations of the SS 433 jets using the Chandra High Energy Transmission Grating Spectrometer with contemporaneous optical and Very Long Baseline Array observations. The X-ray and optical emission line regions are found to be related but not coincident as the optical line emission persists for days while the X-ray emission lines fade in less than 5000 s. The line Doppler shifts from the optical and X-ray lines match well, indicating that they are less than 3 × 10[superscript 14] ...

  2. Deceleration of SS433 radio jets

    CERN Document Server

    Panferov, A A

    2013-01-01

    The mildly relativistic jets of SS\\,433 are believed to inflate the surrounding supernova remnant W\\,50 depositing in its expansion possibly more than 99% of their kinetic energy (\\cite[1998]{Dub98}). Where and how this transformation of energy is curried out, it is not yet known. What can we learn from it that the jets decelerate and the deceleration is non-dissipative, i.e. radiatively dark. In this paper we unclose the observed deviations of the precessing radio jets of SS\\,433, within a few arcseconds from a jets source, from the ballistic track, described by the kinematic model, as a signature of the deceleration which, on other hand, issues from the jets colliding with ambient medium. For that we model kinematics of these colliding jets. The ram pressure on the jets is estimated from the observed profile of brightness of synchrotron radiation along the radio jets. We have found that to fit observed locus the radio jets should be decelerated and twisted, additionally to the precession twist, mostly withi...

  3. Thermal release of tritium from SS316

    International Nuclear Information System (INIS)

    In an effort to improve current understanding of the mechanisms controlling the long-term release of tritium incorporated thermally into stainless steel SS316 and to develop reliable as well as economically feasible techniques for the conditioning of tritium-containing metallic wastes, a systematic investigation is underway in Toyama under carefully controlled conditions. The release rate of tritium from SS316 at ambient pressure was determined experimentally in a flow system at several constant temperatures within the range 287-573 K for rather extended periods of time. Under these conditions HTO was found to constitute by far the most important tritium-containing species being released, i.e. approx. 99 %. Much data has accumulated in recent years with a variety of specimens, i.e. type of stainless steel and specimen dimension, loaded with tritium under different pressure and temperature conditions. Dynamic behavior of long-term tritium release has been successfully modeled using a onedimensional diffusion equation and assuming that the release rate is governed by the tritium flux at the metal surface boundary. The implications of the results for interim storage and thermal conditioning of stainless steel waste will be discussed. (orig.)

  4. Fast optical variability of SS 433

    CERN Document Server

    Burenin, R A; Khamitov, I M; Bikmaev, I F; Nosov, A S; Pavlinsky, M N; Sunyaev, R A

    2011-01-01

    We study the optical variability of the peculiar Galactic source SS 433 using the observations made with the Russian Turkish 1.5-m telescope (RTT150). A simple technique which allows to obtain high-quality photometric measurements with 0.3-1 s time resolution using ordinary CCD is described in detail. Using the test observations of nonvariable stars, we show that the atmospheric turbulence introduces no significant distortions into the measured light curves. Therefore, the data obtained in this way are well suited for studying the aperiodic variability of various objects. The large amount of SS 433 optical light curve measurements obtained in this way allowed us to obtain the power spectra of its flux variability with a record sensitivity up to frequencies of ~0.5 Hz and to detect its break at frequency =~2.4e-3 Hz. We suggest that this break in the power spectrum results from the smoothing of the optical flux variability due to a finite size of the emitting region. Based on our measurement of the break frequ...

  5. Multiwavelength Observations of the SS 433 Jets

    CERN Document Server

    Marshall, Herman L; Hillwig, Todd; Mioduszewski, Amy; Rupen, Michael; Schulz, Norbert S; Nowak, Michael; Heinz, Sebastian

    2013-01-01

    We present observations of the SS 433 jets using the Chandra High Energy Transmission Grating Spectrometer with contemporaneous optical and VLBA observations. The X-ray and optical emission line regions are found to be related but not coincident as the optical line emission persists for days while the X-ray emission lines fade in less than 5000 s. The line Doppler shifts from the optical and X-ray lines match well, indicating that they are less than 3e14 cm apart. The jet Doppler shifts show aperiodic variations that could result from shocks in interactions with the local environment. These perturbations are consistent with a change in jet direction but not jet speed. The proper motions of the radio knots match the kinematic model only if the distance to SS 433 is 4.5 +/- 0.2 kpc. Observations during eclipse show that the occulted emission is very hard, seen only above 2 keV and rising to comprise >50% of the flux at 8 keV. The soft X-ray emission lines from the jet are not blocked, constraining the jet lengt...

  6. The Research on rDNA ITS Sequences of Four Strains of Agaricus blazei%4个姬松茸菌株rDNA ITS序列比较

    Institute of Scientific and Technical Information of China (English)

    宋瑞清; 余江勇; 冀瑞卿; 胡伟; 李喜梅

    2007-01-01

    应用通用引物ITS1和ITS4从4个姬松茸(Agaricus blazei Murill.)菌株子实体中扩增出rDNA ITS序列(登录号分别为EF195648,EF471305,EF471306,EF471307),4菌株ITS序列长度分别为734bp、700bp、724bp和735bp.通过GenBank中BLAST,四个姬松茸菌株与A.brasiliensis(AJ884651)和A.blazei(AF161013)相似率最高,分别为:94%、99%、97%和98%.四个姬松茸菌株同源性较高,达93%以上,其中,ABM-B与ABM-D相似率最高,为97%;ABM-B与ABM-C相似率为96%,ABM-A与ABM-C、ABM-C与ABM-D相似率为94%,ABM-A与ABM-D相似率为93%.

  7. Variações de cor e atividade de água em Agaricus blazei desidratado e armazenado em difere ntes embalagens plásticas = Changes of color and water activity in dehydrated Agaricus blazei mushrooms, stored in different plastic packings

    Directory of Open Access Journals (Sweden)

    Silvia Regina de Toledo Valentini

    2005-01-01

    Full Text Available Neste trabalho foi avaliado o efeito de diferentes embalagens plásticas na atividade de água, na cor e no desenvolvimento microbiano em A. blazei desidratado durante o armazenamento. Amostras de 50 g de cogumelos secos foram embaladas em polipropileno (60 mm, polietileno de baixa densidade (50 mm, laminado duplo de polipropileno biorientado (44 mm e laminado duplo de polipropileno biorientado metalizado (40 mm e armazenadas por 0, 45, 90, 135, 180, 225 e 270 dias a 25°C e 80%UR. Análises microbiológicas de bolores e leveduras foram realizadas antes e após a armazenagem. A embalagem que promoveu maiores aumentos na atividade de água e no escurecimento dos basidiomas foi a de polipropileno, seguida das embalagens de polietileno de baixa densidade e de polipropileno bi-orientado duplo. A embalagem de polipropileno biorientado metalizado duplo proporcionou retenção de cor e não houve aumentos no desenvolvimentode bolores e leveduras nos cogumelos, durante o armazenamento, em nenhuma das embalagens plásticas estudadas.This study evaluated the effect of different plastic packings on dry A. blazei mushrooms, in terms of water activity, color and microbial growth during storage. Samples of 50 g dry mushrooms were wrapped inpolypropylene (60 mm, low-density polyethylene (50 mm, double laminated biaxially oriented polypropylene (44 mm and double laminated metallic biaxially oriented polypropylene (40 mm. Storage periods lasted 0, 45, 90, 135, 180, 225 and 270 days at 25°C and 80%RH. Microbiological analyses of mould and yeasts were carried out before and after storage period. The results showed that the polypropylene packing promoted the greatest increase of water activity and browning in the mushrooms, followed by the low-densitypolyethylene and the double biaxially oriented polypropylene packings. Storage in the double metallic biaxially oriented polypropylene packing retained the color. There was no increase, though, in the development of

  8. Kinetics of polyphenol oxidase in Agaricus blazei%姬松茸多酚氧化酶的动力学特性

    Institute of Scientific and Technical Information of China (English)

    刘文聪

    2009-01-01

    测定了姬松茸不同部位的多酚氧化酶(PPO)活性,研究了不同pH、温度、底物浓度和抑制剂对PPO活性的影响,并建立酶促褐变反应动力学方程.结果表明:在邻苯二酚为底物的条件下,姬松茸PPO的最适pH为6.4,最适温度为30 ℃,米氏常数(K_m)为6.23×10~(-3) mol·L~(-1),最大反应速度(V_(max))为1.42 mol·L~(-1)·min~(-1);柠檬酸对PPO的抑制效果不明显,NaHSO_3和异V_C-Na对PPO均有较强的抑制作用,且有效抑制的含量分别为0.18%和0.21%.%The polyphenol oxidase (PPO) activities in different parts of Agaricus blazei Murrill were determined, the effects of pH value, temperature, substrate concentration and enzyme inhibitors on PPO were studied, too. The results showed that the optimum pH value and optimum temperature for PPO were pH 6.4 and 30 ℃, respectively, the Michaelis constant (K_m) and V_(max) of PPO with pyrocatechol substrate were 6.23 × 10~(-3) mol · L~(-1) and 1.42 mol · L~(-1) · min~(-1) respectively using catechol as substrates, and the reaction kinetic equation was established. The inhibition of citric acid on PPO was not satisfactory, NaHSO_3 and sodium d-isoascorbate could inhibit effectively, and the effective concentrations were 0.18% and 0.21% , respectively.

  9. Kidney Function Indices in Mice after Long Intake of Agaricus brasiliensis Mycelia (=Agaricus blazei, Agaricus subrufescens Produced by Solid State Cultivation

    Directory of Open Access Journals (Sweden)

    Dalla S. O. Roberto

    2009-01-01

    Full Text Available Problem statement: Agaricus brasiliensis (=Agaricus blazei, Agaricus subrufescens or Sun mushroom has widespread use for potential health benefits such anti-tumor and immunomodulatory effects. Studies detected that others edible mushrooms affected renal metabolism and despite the widespread use of A. brasiliensis there are no studies that address biological effects on the renal function indices after their oral administration. Therefore, this study had as objective to verify the effects on kidney function indices after long intake of A. brasiliensis mycelium. Approach: Wheat grains was cultured during 18 days with Agaricus brasiliensis mycelium by solid state culture and used for chown formulation. Groups of female Swiss mice (20 per group were fed during 14 weeks with 100 and 50% of the formulated feed denominated A100 and A50, respectively. Control group received formulated chown with wheat grains without mycelium. The water intake and excreted urine volume; the physic chemistry analysis of the urine and the serum levels of glucose, proteins, urea, creatinin and uric acid was determined (Meditron Junior-Boehringer, reagent strips Combur 10; microscopy and ADVIA 1650 Bayer. Results: A100 and A50 groups ingested 19.1 and 15.8% more water compared to C group, respectively. The urine and serum analysis showed that the verified parameters remained invariables for all groups, including glucose levels, which resulted in a 10% reduction of A100 group, without statistical difference (p>0.05 Vs C. Conclusion/Recommendations: The prolonged intake of supplemented feed with A. brasiliensis mycelium didn’t result in indicative alterations in the kidney function indices. The preventive use of the mushroom did not show any deleterious effects on kidney; however complementary studies are necessary to guarantee complete safety; possible correlation between increase of urinary excretion and hypotensive effect

  10. Accretion disk structure in SS Cygni

    Science.gov (United States)

    Hessman, F. V.

    1987-02-01

    High-resolution coude observations of nonaxisymmetric line emission from the dwarf nova SS Cygni are presented. By subtracting the constant line component, the asymmetric line emission responsible for the observed phase shift between the absorption and emission line radial velocity curves can be isolated. The extra emission is a large fraction of the total line emission and extends to large velocities (of about 1500 km/sec). The phase stability of the emission demands a large-scale structure which is fixed in the frame of the binary. A magnetic origin of the excitation cannot be ruled out but is implausible. A simple explanation is that the accretion stream from the companion star is able to spill over the edge of the disk, introducing emission at noncircular velocities and most likely disturbing the upper layers of the accretion disk.

  11. MULTIWAVELENGTH OBSERVATIONS OF THE SS 433 JETS

    Energy Technology Data Exchange (ETDEWEB)

    Marshall, Herman L.; Canizares, Claude R.; Schulz, Norbert S.; Nowak, Michael [Kavli Institute for Astrophysics and Space Research, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139 (United States); Hillwig, Todd [Department of Physics and Astronomy, Valparaiso University, Valparaiso, IN 46383 (United States); Mioduszewski, Amy; Rupen, Michael [NRAO, P.O. Box 2, Socorro, NM 87801 (United States); Heinz, Sebastian, E-mail: hermanm@space.mit.edu, E-mail: crc@space.mit.edu, E-mail: nss@space.mit.edu, E-mail: mnowak@space.mit.edu, E-mail: todd.hillwig@valpo.edu, E-mail: amiodusz@nrao.edu, E-mail: mrupen@aoc.nrao.edu, E-mail: heinzs@astro.wisc.edu [Astronomy Department, 5408 Sterling Hall, University of Wisconsin, Madison, WI 53706 (United States)

    2013-09-20

    We present observations of the SS 433 jets using the Chandra High Energy Transmission Grating Spectrometer with contemporaneous optical and Very Long Baseline Array observations. The X-ray and optical emission line regions are found to be related but not coincident as the optical line emission persists for days while the X-ray emission lines fade in less than 5000 s. The line Doppler shifts from the optical and X-ray lines match well, indicating that they are less than 3 × 10{sup 14} cm apart. The jet Doppler shifts show aperiodic variations that could result from shocks in interactions with the local environment. These perturbations are consistent with a change in jet direction but not jet speed. The proper motions of the radio knots match the kinematic model only if the distance to SS 433 is 4.5 ± 0.2 kpc. Observations during eclipse show that the occulted emission is very hard, seen only above 2 keV and rising to comprise >50% of the flux at 8 keV. The soft X-ray emission lines from the jet are not blocked, constraining the jet length to ∼> 2 × 10{sup 12} cm. The base jet density is in the range 10{sup 10-13} cm{sup –3}, in contrast to our previous estimate based on the Si XIII triplet, which is likely to have been affected by UV de-excitation. There is a clear overabundance of Ni by a factor of about 15 relative to the solar value, which may have resulted from an unusual supernova that formed the compact object.

  12. Multiwavelength Observations of the SS 433 Jets

    Science.gov (United States)

    Marshall, Herman L.; Canizares, Claude R.; Hillwig, Todd; Mioduszewski, Amy; Rupen, Michael; Schulz, Norbert S.; Nowak, Michael; Heinz, Sebastian

    2013-09-01

    We present observations of the SS 433 jets using the Chandra High Energy Transmission Grating Spectrometer with contemporaneous optical and Very Long Baseline Array observations. The X-ray and optical emission line regions are found to be related but not coincident as the optical line emission persists for days while the X-ray emission lines fade in less than 5000 s. The line Doppler shifts from the optical and X-ray lines match well, indicating that they are less than 3 × 1014 cm apart. The jet Doppler shifts show aperiodic variations that could result from shocks in interactions with the local environment. These perturbations are consistent with a change in jet direction but not jet speed. The proper motions of the radio knots match the kinematic model only if the distance to SS 433 is 4.5 ± 0.2 kpc. Observations during eclipse show that the occulted emission is very hard, seen only above 2 keV and rising to comprise >50% of the flux at 8 keV. The soft X-ray emission lines from the jet are not blocked, constraining the jet length to >~ 2 × 1012 cm. The base jet density is in the range 1010-13 cm-3, in contrast to our previous estimate based on the Si XIII triplet, which is likely to have been affected by UV de-excitation. There is a clear overabundance of Ni by a factor of about 15 relative to the solar value, which may have resulted from an unusual supernova that formed the compact object.

  13. Molecular dynamics simulations of a single stranded (ss) DNA

    CERN Document Server

    Chatterjee, Subhasish; Thakur, Siddarth; Burin, Alexander

    2012-01-01

    The objective of the present study was to develop an understanding of short single-stranded DNA (ssDNA) to assist the development of new DNA-based biosensors. A ssDNA model containing twelve bases was constructed from the 130-145 codon sequence of the p53 gene. Various thermodynamic macroscopic observables such as temperature, energy distributions, as well as root mean square deviation (RMSD) of the nucleic acid backbone of the ssDNA were studied using molecular dynamics (MD) simulations. The AMBER program was used for building the structural model of the ssDNA, and atomistic MD simulations in three different ensembles were carried out using the NAMD program. The microcanonical (NVE), conical (NVT) and isobaric-isothermal (NPT) ensembles were employed to compare the equilibrium characteristics of ssDNA in aqueous solutions. Our results indicate that the conformational stability of the ssDNA is dependent on the thermodynamic conditions.

  14. Tukiasumisyksikössä asuvien maahanmuuttajanuorten kokemukset kotoutumisesta

    OpenAIRE

    Haataja, Auri-Elina; Tampio, Sanna

    2012-01-01

    Haataja, Auri & Tampio, Sanna. Tukiasumisyksikössä asuvien maahanmuuttajanuorten kokemukset kotoutumisesta. Diak Pohjoinen, Oulu, kevät 2012, 61 s., 1 liite. Diakonia-ammattikorkeakoulu, Sosiaalialan koulutusohjelma, Diakonisen sosiaalityön suuntautumisvaihtoehto, sosionomi (AMK) + diakoni. Opinnäytetyön tarkoituksena oli kuvata tukiasumisyksikössä asuvien maahanmuuttajanuorten kokemuksia kotoutumisesta Pudasjärvelle. Opinnäytetyön tavoitteena oli tuottaa tietoa tukiasumisyksikössä asu...

  15. The Spectral Components of SS 433

    Science.gov (United States)

    Gies, D. R.; McSwain, M. V.; Riddle, R. L.; Wang, Z.; Wiita, P. J.; Wingert, D. W.

    2002-02-01

    We present results from new optical and UV spectroscopy of the unusual binary system SS 433, and we discuss the relationship of the particular spectral components that we observe to the properties of the binary. These spectral components include1. The continuum spectrum, which we associate with flux from the super-Eddington accretion disk and the dense part of its wind. A far-UV spectrum from the Hubble Space Telescope Space Telescope Imaging Spectrograph made during the edge-on orientation of the disk places an upper limit on the temperature of an equivalent blackbody source (Trecession (like the velocity curve of He II λ4686). This is probably formed in outflow from the central region of the disk near the compact star.5. Absorption and emission features from outflowing clumps in the disk wind (seen most clearly in an episode of blueshifted Na I emission).6. We found no clear evidence of the absorption-line spectrum of the optical star, although we point out the presence of He I absorption features (blended with the stationary emission) with the expected radial velocity trend at the orbital and precessional phases when the star might best be seen.7. A rich interstellar absorption spectrum of diffuse interstellar bands.The results suggest that the binary is embedded in an expanding thick disk (detected in recent radio observations) that is fed by the wind from the super-Eddington accretion disk.

  16. The reproducible radio outbursts of SS Cygni

    CERN Document Server

    Russell, T D; Sivakoff, G R; Altamirano, D; O'Brien, T J; Page, K L; Templeton, M R; Koerding, E G; Knigge, C; Rupen, M P; Fender, R P; Heinz, S; Maitra, D; Markoff, S; Migliari, S; Remillard, R A; Russell, D M; Sarazin, C L; Waagen, E O

    2016-01-01

    We present the results of our intensive radio observing campaign of the dwarf nova SS Cyg during its 2010 April outburst. We argue that the observed radio emission was produced by synchrotron emission from a transient radio jet. Comparing the radio light curves from previous and subsequent outbursts of this system (including high-resolution observations from outbursts in 2011 and 2012) shows that the typical long and short outbursts of this system exhibit reproducible radio outbursts that do not vary significantly between outbursts, which is consistent with the similarity of the observed optical, ultraviolet and X-ray light curves. Contemporaneous optical and X-ray observations show that the radio emission appears to have been triggered at the same time as the initial X-ray flare, which occurs as disk material first reaches the boundary layer. This raises the possibility that the boundary region may be involved in jet production in accreting white dwarf systems. Our high spatial resolution monitoring shows th...

  17. The anti-tumorigenic mushroom Agaricus blazei Murill enhances IL-1β production and activates the NLRP3 inflammasome in human macrophages.

    Directory of Open Access Journals (Sweden)

    Tsung-Teng Huang

    Full Text Available Agaricus blazei Murill (AbM has been reported to possess immune activity against tumors and infections through stimulation of mononuclear phagocytes. Recently, AbM extract was shown to induce the production of the pro-inflammatory cytokine, interleukin-1β (IL-1β, in human monocytes. IL-1β is a key pro-inflammatory cytokine produced by activated macrophages and monocytes and its secretion is strictly controlled by the inflammasome. The purpose of this study is to investigate the effect of AbM water extracts on the regulation of IL-1β production and activation of the NLRP3 inflammasome in human THP-1 macrophages. The NLRP3 inflammasome consists of an NLRP3 receptor, an adaptor protein called ASC, and the inflammatory protease, caspase-1. Typically, stimulation of immune cells with microbial products results in production of pro-IL-1β, but a second stress-related signal activates the inflammasome and caspase-1, leading to processing and secretion of IL-1β. Our results show that AbM enhances transcription of IL-1β and triggers NLRP3 inflammasome-mediated IL-1β secretion in human THP-1 macrophages. AbM-mediated IL-1β secretion was markedly reduced in macrophages deficient in NLRP3 and ASC, demonstrating that the NLRP3 inflammasome is essential for AbM-induced IL-1β secretion. In addition, caspase-1 was activated and involved in proteolytic cleavage and secretion of IL-1β in AbM-treated macrophages. AbM-mediated IL-1β secretion also decreased in cells treated with cathepsin B inhibitor, suggesting that AbM can induce the release of cathepsin B. Furthermore, our data show that AbM-induced inflammasome activation requires the release of ATP, binding of extracellular ATP to the purinergic receptor P2X(7, the generation of reactive oxygen species, and efflux of potassium. Taken together, these findings reveal that AbM activates the NLRP3 inflammasome via multiple mechanisms, resulting in the secretion of IL-1β.

  18. Mechanochemical regulations of RPA's binding to ssDNA

    Science.gov (United States)

    Chen, Jin; Le, Shimin; Basu, Anindita; Chazin, Walter J.; Yan, Jie

    2015-03-01

    Replication protein A (RPA) is a ubiquitous eukaryotic single-stranded DNA (ssDNA) binding protein that serves to protect ssDNA from degradation and annealing, and as a template for recruitment of many downstream factors in virtually all DNA transactions in cell. During many of these transactions, DNA is tethered and is likely subject to force. Previous studies of RPA's binding behavior on ssDNA were conducted in the absence of force; therefore the RPA-ssDNA conformations regulated by force remain unclear. Here, using a combination of atomic force microscopy imaging and mechanical manipulation of single ssDNA tethers, we show that force mediates a switch of the RPA bound ssDNA from amorphous aggregation to a much more regular extended conformation. Further, we found an interesting non-monotonic dependence of the binding affinity on monovalent salt concentration in the presence of force. In addition, we discovered that zinc in micromolar concentrations drives ssDNA to a unique, highly stiff and more compact state. These results provide new mechanochemical insights into the influences and the mechanisms of action of RPA on large single ssDNA.

  19. Extending OmpSs to support dynamic programming

    OpenAIRE

    Maroñas Bravo, Marcos

    2015-01-01

    [CASTELLÀ] Tras un breve resumen sobre programación dinámica y OmpSs, este documento muestra como OmpSs ofrece soporte para memoización y los resultados obtenidos. Se detallan las extensiones del modelo, del compilador Mercurium y del runtime Nanos++ realizadas para soportar memoización.

  20. Turboahtimen mitoitus polttomoottorikäytössä

    OpenAIRE

    Rantanen, Jyri

    2014-01-01

    Tämä opinnäytetyö käsittelee turboahtimen mitoittamiseen liittyvää teoriaa, sekä käytäntöä polttomoottorikäyttöisissä autoissa. Työssä käydään lävitse tarvittavat laskukaavat, joiden avulla saadaan apua oikeanlaisen ahtimen valintaan. Tässä työssä keskitytään enemmän ahtimen valintaan moottorin virityskäytössä, kuin suurimman mahdollisen käytettävyyden sekä polttoainetalouden saavuttamiseen. Opinnäytetyössä lasketaan teoreettisilla kaavoilla erään ahtimen sopivuus tietylle autolle. Lopuks...

  1. Toivo sairaanhoitajan työssä : Sairaanhoitajien kokemuksia toivosta hoitotyössä

    OpenAIRE

    Lehtinen, Katri

    2011-01-01

    Tämän opinnäytetyön tarkoituksena oli kuvata, minkä sisällön sairaanhoitajat antoivat käsitteelle toivo ja miten he toivon kokivat. Opinnäytetyön tavoitteena oli tuoda näkyväksi sairaanhoitajien kokemukset toivosta omassa työssä. Saadun tiedon perusteella toivo on lähtökohta sairaanhoitajan työn toteutumiselle ja potilaan toipumiselle sairaudestaan. Opinnäytetyö toteutui kvalitatiivisen tutkimusmenetelmän mukaisesti. Aineisto kerättiin haastattelemalla sairaanhoitajia. Sain tietoa haastat...

  2. Laitteiden testauksessa kulutettu energia Vacon Oyj:ssä

    OpenAIRE

    Mustonen, Tero

    2013-01-01

    Vacon Oyj on osana energiansäästökampanjaa, jonka tavoitteena on vähentää energiankulutusta 20 % vuoteen 2016 mennessä.Tässä työssä tutkitaan Vacon Oyj:n taajuusmuuttajien ja inverttereiden testaamiseen kuluvien energioiden suhteita. Työssä mitattiin testattavien laitteiden verkosta otettavan energian ja testauspiirissä kierrätettävän energian suhdetta eri testauskonsepteissa. Työhön kuului mittauslaitteistojen käyttöönotto sekä uusien suunnittelu ja rakentaminen. Tulokset kirjattiin ylös työ...

  3. Langaton tiedonsiirto sulautetussa järjestelmässä

    OpenAIRE

    Rosila, Ville

    2014-01-01

    ssä opinnäytetyöraportissa käsitellään langattoman tiedonsiirron toteuttamista erääseen sulautettuun järjestelmään. Työn tärkeimpänä tavoitteena oli saada langaton tiedonsiirto toimimaan järjestelmässä tehokkaasti ja luotettavasti. Toisena tavoitteena oli toteuttaa jokin käytännönläheinen esimerkkisovellus, jossa langattomasta tiedonsiirrosta on hyötyä. Raportissa perehdytään työssä käytettyihin komponentteihin ja niiden oleellisimpiin teknisiin ominaisuuksiin. Komponenttien lisäksi esitell...

  4. Työssä pysyminen Keravan vankilassa

    OpenAIRE

    Yli-Yrjänäinen, Satu

    2013-01-01

    Tämän opinnäytetyön tarkoituksena oli selvittää työntekijöiden itsensä kertomana niitä tekijöitä, jotka ovat olleet merkityksellisiä heidän työssä pysymiseensä Keravan vankilassa. Tutkimuskysymys oli: Millaiset tekijät saavat työntekijän pysymään työssä Keravan vankilassa? Teoreettisessa viitekehyksessä esitellään lyhyesti muiden työssä pysymistä kartoittaneiden tutkimusten keskeisiä tuloksia. Laajemmin avataan yhdysvaltalaisen vankeinhoidon työssä pysymiseen liittyviä tekijöitä. Teoreettises...

  5. Hybrid MPI/StarSs - a case study

    OpenAIRE

    Gracia, Jose; Niethammer, Christoph; Hasert, Manuel; Brinkmann, Steffen; Keller, Rainer; Glass, Colin W.

    2012-01-01

    Hybrid parallel programming models combining distributed and shared memory paradigms are well established in high-performance computing. The classical prototype of hybrid programming in HPC is MPI/OpenMP, but many other combinations are being investigated. Recently, the data-dependency driven, task parallel model for shared memory parallelisation named StarSs has been suggested for usage in combination with MPI. In this paper we apply hybrid MPI/StarSs to a Lattice-Boltzmann code. In particul...

  6. 姬松茸60Co辐射新菌株J3营养成分与农药残留分析%Analysis on Nutrients and Pesticide Residues in Strain J3 Agaricus blazei Murill Irradiated by 60Co

    Institute of Scientific and Technical Information of China (English)

    翁伯琦; 江枝和; 肖淑霞; 雷锦桂; 王义祥; 唐翔虬

    2011-01-01

    通过常规生产床栽,对比研究姬松茸Co辐射新菌株J和原菌株J在营养品质以及重金属含量和农药残留情况.结果表明,姬松茸Co辐射新菌株J子实体中鲜味氨基酸总量(56.5 g·kg)、甜味氨基酸总量(52.4g·kg)、硫氨基酸总量(15.8 g·kg)、支链氨基酸总量(38.1 g·kg)、芳香族氨基酸总量(16.1 g·kg)、儿童氨基酸总量(17.8 g·kg)、必需氨基酸总量(95 g·kg),分别占氨基酸总量(221.7 g·kg)的25.48%、23.64%、7.13%、17.19%、7.26%、8.03%和42.85%,分别比姬松茸J高28.12%、12.93%、0%、14.41%、12.59%、16.34%和11.76%.J的多不饱和脂肪酸(76.15%)和不饱和脂肪酸(77.55%)分别比姬松茸原菌株J高4.39%和3.82%.J子实体含镉(3.86 mg·kg)、汞(0.42 mg·kg)和砷(0.09 mg·kg),也分别比原菌株J低45.86%、32.25%和18.18%;其铅含量符合国家食用菌卫生标准GB 7096-2003;砷含量符合国家绿色食品食用菌NY/T749-2003的标准.子实体中联苯菊酯、溴氰菊酯、百菌清、六六六、多菌灵、阿维菌素、甲基托布津、甲胺磷、毒死蜱、滴滴涕和二氧化硫含量均符合国家绿色食品食用菌NY/T 749-2003的标准.%A garicus blazei Murill as rare edible fungi was adored by more and more customers.But with the increasing attention of food hygiene quality, it was important guidance of the safety production of Agaricus blazei to understand the health status of Agaricus blazei in the actual cultivation.This paper studied the difference in terms of nutritional quality, heavy metal contents and pesticide residues between strain J3 and J1.The results showed that the contents of delicious amino acid(56.5 g· kg-1), sweet amino acid(52.4 g· kg-1), sulfur amino acid( 15.8 g·kg-1), branched chain amino acid(38.1 g· kg-1), aromatic amino acid( 16.1 g·kg-1), children amino acid( 17.8 g·kg-1), necessary amino acid (95.0 g·kg-1)in the fruit bodies ofAgaricus blazei J3, occupied 25.48%, 23.64%, 7.13%, 17.19%, 7.26

  7. Performance of DMFC with SS 316 bipolar/end plates

    Science.gov (United States)

    Padhy, Biswa R.; Reddy, Ramana G.

    This work mainly emphasizes the development of new materials and design for a bipolar/end plate in a direct methanol fuel cell (DMFC). According to the DOE requirements, preliminary studies show that SS 316 (Stainless Steel 316) is a suitable candidate. Several flow field designs were studied and a modified serpentine design was proposed. SS 316 end plates were fabricated with an intricate modified serpentine flow field design on it. The performance of a single stack DMFC with SS 316 end plates were studied with different operational parameters. A long-term test was carried out for 100 h with recycling the methanol and the contaminants in the MEA were characterized. The stack efficiency is found to be 51% and polarization losses are discussed. SS 316 with low permeability resulted in an increased pressure drop across the flow field, which increased the fuel cell performance. The use of SS 316 as bipolar plate material will reduce the machining cost as well as volume of the fuel cell stack.

  8. Optimisation of hot isostatic pressing bonded SS/SS joints conditions for ITER blanket shield

    International Nuclear Information System (INIS)

    In the engineering design activity of international thermonuclear experimental reactor (ITER), stainless steels are being considered as candidates materials for several module type structures. Hot isostatic pressing (HIP) technique is expected for the fabrication of these modules. Stainless steel powders are simultaneously consolidated as mono-material block or/and joined in bi-material module. This paper reviews the manufacturing stages, non-destructive examination and the developments of the HIP bonded joints of 316L SS (powder and solid) for application to the ITER shield blanket. It is well known that the powder surface oxidation negatively influences the impact toughness of raw material and joints consolidated by this way. In order to get acceptable mechanical properties of materials, a study on the effect of reducing the powder oxygen content has been launched. To evaluate susceptibility to the oxygen content of HIPed joint specimens, tensile and toughness tests have been performed. From this study, optimal conditions of HIP were fitted and the influence of oxygen was mastered to obtain good mechanical properties of the consolidated powder material as well as for HIPed junction.

  9. Hybrid MPI/StarSs - a case study

    CERN Document Server

    Gracia, Jose; Hasert, Manuel; Brinkmann, Steffen; Keller, Rainer; Glass, Colin W

    2012-01-01

    Hybrid parallel programming models combining distributed and shared memory paradigms are well established in high-performance computing. The classical prototype of hybrid programming in HPC is MPI/OpenMP, but many other combinations are being investigated. Recently, the data-dependency driven, task parallel model for shared memory parallelisation named StarSs has been suggested for usage in combination with MPI. In this paper we apply hybrid MPI/StarSs to a Lattice-Boltzmann code. In particular, we present the hybrid programming model, the benefits we expect, the challenges in porting, and finally a comparison of the performance of MPI/StarSs hybrid, MPI/OpenMP hybrid and the original MPI-only versions of the same code.

  10. Toimitusjohtajan rooli ja asema perheyhtiössä

    OpenAIRE

    Fontell, Marleena

    2015-01-01

    Toimitusjohtajan rooli ja asema ovat usein epäselviä. Erityisesti pienissä perheyrityksissä toimitusjohtajan rooli voi olla epämääräinen, tai sitä ei ole erikseen määritelty. Opinnäytetyössä tarkastellaan toimitusjohtajan roolia ja asemaa perheyrityksessä X, jossa on tapahtunut sukupolvenvaihdos. Tutkimuksessa haluttiin tuoda esiin sopimusoikeudellinen puoli, ovatko toimitusjohtajan toimet lainsäädännön mukaisia vai onko hän enemmän työntekijän roolissa. Opinnäytetyössä tutkittiin, mikä o...

  11. Ammatillisen vuorovaikutuksen tukeminen ohjaustyössä

    OpenAIRE

    Lappalainen, Sari; Kurppa, Jatta

    2016-01-01

    Tämä opinnäytetyö on toiminnallinen opinnäytetyö. Sen aiheena oli ammatillisen vuorovaikutuksen tukeminen ohjaustyössä. Tavoitteena oli herättää ajatuksia ja laajentaa näkökulmaa ammatillisissa vuorovaikutustilanteissa. Opinnäytetyön teoreettiseksi viitekehykseksi valittiin teorioita, jotka olennaisesti vaikuttavat ammatillisen vuorovaikutuksen toteutumiseen ohjaustyössä. Opinnäytetyön toimeksiantajana toimi AMF Vastaanottopalvelut. Toiminnallinen osuus toteutettiin koko työyhteisölle yh...

  12. Improving the online marketing of Homekoirat S&S

    OpenAIRE

    Virkki, Sandra

    2016-01-01

    The aim of this thesis is to improve the online marketing of an already existing company, Homekoirat S&S and to research what are the best marketing tools for marketing a ser-vice online. Also a suggestion of the marketing plan will be created. Homekoirat S&S is a small company located in Mikkeli, Finland and it’s founded in the beginning of 2012 by owners Saara Pulkkinen and Sampsa Väntsi. The company provides mold detecting services by using sniffer dogs that are trained using the simi...

  13. Hoitajien kokemuksia omahoitajuudesta psykiatrisessa hoitotyössä

    OpenAIRE

    Heinonen, Noora; Viita-aho, Jori

    2014-01-01

    Oulun seudun ammattikorkeakoulu Hoitotyön koulutusohjelma Tekijät: Noora Heinonen ja Jori Viita-aho Opinnäytetyön nimi: Hoitajien kokemuksia omahoitajuudesta psykiatrisessa hoitotyössä Työn ohjaaja: Liisa Kiviniemi Valmistumisaika: Kevät 2014 Sivumäärä: 37 Opinnäytetyön tarkoituksena oli kuvailla hoitajien kokemuksia omahoitajuudesta psykiatrisessa hoitotyössä. Opinnäytetyön tavoitteena oli tuottaa kokemusperäistä tietoa omahoitajuuden toteuttamisesta ja sisällö...

  14. ICT:n rooli toimitusketjuyhteistyössä

    OpenAIRE

    Fagerström, Marko

    2013-01-01

    Opinnäytetyö on osa SULOIN-hanketta, jossa haetaan uusia toimintamalleja elintarviketeollisuuden jakelukanavien kehittämiseen. Opinnäytetyössä tutkittiin, mikä on ICT:n rooli toimitusketjuyhteistyössä, ja minkälainen vaikutus sillä on jakelukanavan markkina- ja neuvotteluvoimiin. Tutkimusstrategiana käytettiin kvalitatiivista tutkimusta, ja pääasiallinen tutkimusmenetelmä oli kohdeyrityksissä tehdyt teemahaastattelut. Yrityksiä, joissa haastattelut tehtiin, oli kolme. Näistä kaksi on elin...

  15. YouTube osuuskaupan markkinointiviestinnässä

    OpenAIRE

    Lindevall, Tytti

    2014-01-01

    Tämän opinnäytetyön tavoitteena on luoda toimeksiantaja Suur-Seudun Osuuskauppa SSO:lle suunnitelma YouTuben hyödyntämiseksi markkinointiviestinnässään. Työn avulla on tarkoitus löytää keino, miten SSO voisi hyödyntää YouTubea markkinointiviestinnässään ja mitä asioita tulisi huomioida omaa kanavaa perustettaessa. Tarkoituksena on tuottaa raportti mahdollisista käyttötarkoituksista sekä ohjeistus oman kanavan perustamiseen ja hallinnointiin liittyvistä asioista. Työn alkupuolella taustoite...

  16. Sosiaalinen vahvistaminen avoimessa nuorisotilatyössä

    OpenAIRE

    Santanen, Lotta-Julia; Nieminen, Katri

    2013-01-01

    Toiminnallinen opinnäytetyö toteutettiin yhteistyössä Jyväskylän kaupungin nuorisotoimen ja Humanistisen ammattikorkeakoulun kanssa syksyllä 2013. Opinnäytetyön tavoitteena oli selvittää millaista on sosiaalinen vahvistaminen avoimessa tilatoiminnassa sekä mikä on työntekijän rooli sosiaalisessa vahvistamisessa uudella tilalla. Tutkimusmenetelmiksi kehiteltiin kaksi sosiaalista vahvistamista tukevaa toiminnallista menetelmää, joita testattiin käytännössä kahdella eri Jyväskylän kaupungin...

  17. Laulaen iloa saamme : musiikki vapaaehtoistyössä

    OpenAIRE

    Herranen, Linda

    2011-01-01

    Vuoden 2009 lopulla Eläkeliitto otti yhteyttä ammattikorkeakouluumme ja kysyi halukkaita tekemään opinnäytetyötä Sävel soikoon –hankkeesta. Hankkeen idea on kouluttaa vapaaehtoisina toimijoita pitämään musiikkituokioita vanhainkodeissa, sairaaloissa ja yksityiskodeissa. Opinnäytetyössäni tarkastelin vapaaehtoistyössä käytettävää musiikkia. Tarkoituksena oli selvittää, millä perusteella vapaaehtoiset valitsevat musiikkituokiossa käytettävät kappaleet, mitä kappaleita vapaaehtoiset käyttävät mi...

  18. e-VLBI observations of SS 433 in outburst

    Science.gov (United States)

    Tudose, V.; Paragi, Z.; Trushkin, S.; Soleri, P.; Fender, R.; Garrett, M.; Spencer, R.; Rushton, A.; Burgess, P.; Kunert-Bajraszewska, M.; Pazderski, E.; Borkowski, K.; Hammargren, R.; Lindqvist, M.; Maccaferri, G.

    2008-11-01

    We have observed the X-ray binary SS 433 on November 6, 2008 between 13:48-18:35 UT at 5 GHz with the European VLBI Network (EVN) using the e-VLBI technique. The radio telescopes participating in the experiment were: Medicina, Onsala 25m, Torun, Jodrell Bank MkII and Cambridge. The X-ray binary SS 433 is in outburst. Trushkin & Nizhelskij (ATel #1819) reported a major flare already active during the RATAN-600 observations in the 1-22 GHz band on 2008 October 28.

  19. Susceptibility to Stress Corrosion Cracking of 254SMO SS

    OpenAIRE

    De Micheli Lorenzo; Agostinho Silvia Maria Leite; Trabanelli Giordano; Zucchi Fabrizio

    2002-01-01

    The susceptibility to stress corrosion cracking (SCC) of solubilized and sensitized 254SMO SS was studied in sodium chloride, and sodium fluoride solutions at 80 °C and sulfuric acid solutions in presence of sodium chloride at 25 °C. The influence of salt concentration, pH values and the addition of thiosulfate was examined. The susceptibility to SCC was evaluated by Slow Strain Rate Tests (SSRT), at 1.5 x 10-6 s-1 strain rate. The behavior of 254SMO was compared to those of AISI 316L SS and ...

  20. Molecular Mapping of Sterility QTLs qSS-3, qSS-6a and qSS-7 as Single Mendelian Factors via NIL strategy

    Institute of Scientific and Technical Information of China (English)

    LI Jing; XU Peng; ZHOU Jia-wu; HU Feng-yi; DENG Xian-neng; CHEN Zhi-wei; TAO Da-yun

    2011-01-01

    Hybrid sterility between Oryza glaberrima and O.sativa seriously hampers the introgression of favorable genes from each other.In order to further understand this issue,identification and isolation of hybrid sterility QTLs as single Mendelian factors are an effective strategy.A genetic map was constructed using a BC1F1 population derived from a cross between an O.sativa japonica cultivar and an O.glaberrima accession.Four main-effect QTLs for pollen sterility were detected in the BC1 F1.Five BC8F1 advanced backcross populations were developed via successive backcrosses based on phenotype and molecular selections.The BC8F1 populations showed bimodal distribution for pollen fertility and could be classified into semi-sterile and fertile types,fitting single Mendilian factor inheritance ratios.Three QTLs detected in the BC1F1 corresponding to qSS-3,qSS-6a and qSS-7 were mapped on chromosomes 6,3 and 7,respectively,as single Mendilian factors.

  1. Turvallisuuteen liittyvien lupaehtojen noudattaminen maantiellä tehtävässä työssä

    OpenAIRE

    Ahola, Mikael

    2011-01-01

    Maantiellä tehtävä työ on luvanvaraista työtä, jota hallinnoivat tienpitoviran-omaisena paikalliset elinkeino-, liikenne- ja ympäristökeskukset. Opinnäytetyön tarkoituksena oli selvittää, miten maantiellä tehtävässä työssä noudatetaan tienpitoviranomaisen antamia työ- ja liikenneturvallisuuteen liittyviä lupaehtoja. Työ tehtiin ELY-keskuksen liikenne- ja infrastruktuuri -vastuualueen kunnossa-pitoyksikössä, joka myöntää luvat maanteillä työskentelyyn. Lähtöaineisto opinnäytetyöhön kerätti...

  2. Yhteisöllisyys nuorten ehkäisevässä päihdetyössä

    OpenAIRE

    Helmiö, Susanna

    2010-01-01

    Yhteisöllisyys nuorten ehkäisevässä päihdetyössä opinnäytetyö toteutettiin Humanistisen ammattikorkeakoulun alaisen ehkäisevän päihdetyön osaamiskeskus Preventiimin hankkeen osakokonaisuutena. Hankkeessa pyrittiin selvittämään nuorten kokemuksia yhteisöllisyydestä ja ehkäisevästä päihdetyöstä. Opinnäytetyössä Espoon nuorisovaltuusto kertoi kokemuksiaan nuorten päihteiden käytöstä ja yhteisöllisyydestä. Nuorisovaltuustolaiset toimivat asiantuntijoina nuorten elämästä, ei oman toimintansa kerto...

  3. Dissociation between the effects of somatostatin (SS) and octapeptide SS-analogs on hormone release in a small subgroup of pituitary- and islet cell tumors

    NARCIS (Netherlands)

    L.J. Hofland (Leo); W.W. de Herder (Wouter); H.A. Visser-Wisselaar (Heleen); C. van Uffelen; M. Waaijers (Marlijn); J. Zuyderwijk; P. Uitterlinden (Piet); P.M. van Koetsveld (Peter); S.W.J. Lamberts (Steven); J.M. Kros (Johan)

    1997-01-01

    textabstractThe effects of somatostatin (SS-14 and/or SS-28) and of the three octapeptide SS-analogs that are available for clinical use (octreotide, BIM-23014 and RC-160) on hormone release by primary cultures of 15 clinically nonfunctioning pituitary adenomas (NFA), 7

  4. Compatibility between Be12Ti and SS316LN

    International Nuclear Information System (INIS)

    Beryllides have good properties and are one of the candidate materials for the neutron multiplier of the DEMO reactor in which good performance is required at 600-800 deg. C. Therefore, a good compatibility with structural materials is expected for the beryllides. In this study, a compatibility test between Be12Ti and SS316LN was carried out as first step to evaluate the compatibility between beryllides and structural materials. The thickness of the reaction layer between Be12Ti and SS316LN at 800 deg. C after 1000 h was approximately 30 μm, whereas that of beryllium metal was 300 μm. At 600 deg. C after 1000 h, the thickness of the reaction layers as to Be12Ti and Be was less than 10 and 100 μm, respectively. The compatibility between Be12Ti and SS316LN was evaluated and Be12Ti was perfectly better than that between beryllium metal and SS316LN at high temperature (600-800 deg. C). The advantage of beryllides as a neutron multiplier in the Demo reactor was proved

  5. Outburst from SS 433 observed with BOOTES-2/COLORES

    Science.gov (United States)

    Caballero-Garcia, M. D.; Castro-Tirado, A.; Jelinek, M.; et al.

    2014-08-01

    In light of the recent extreme outburst of SS 433 (ATELs #6347, #6355) and follow-up by SWIFT (ATEL #6364) the 0.6m TELMA robotic telescope at the BOOTES-2 (+COLORES) astronomical station in Malaga (Spain), obtained optical 4000-9000 A spectra starting at 2014-08-01 and ending at 2014-08-07.

  6. RPA homologs and ssDNA processing during meiotic recombination.

    Science.gov (United States)

    Ribeiro, Jonathan; Abby, Emilie; Livera, Gabriel; Martini, Emmanuelle

    2016-06-01

    Meiotic homologous recombination is a specialized process that involves homologous chromosome pairing and strand exchange to guarantee proper chromosome segregation and genetic diversity. The formation and repair of DNA double-strand breaks (DSBs) during meiotic recombination differs from those during mitotic recombination in that the homologous chromosome rather than the sister chromatid is the preferred repair template. The processing of single-stranded DNA (ssDNA) formed on intermediate recombination structures is central to driving the specific outcomes of DSB repair during meiosis. Replication protein A (RPA) is the main ssDNA-binding protein complex involved in DNA metabolism. However, the existence of RPA orthologs in plants and the recent discovery of meiosis specific with OB domains (MEIOB), a widely conserved meiosis-specific RPA1 paralog, strongly suggest that multiple RPA complexes evolved and specialized to subdivide their roles during DNA metabolism. Here we review ssDNA formation and maturation during mitotic and meiotic recombination underlying the meiotic specific features. We describe and discuss the existence and properties of MEIOB and multiple RPA subunits in plants and highlight how they can provide meiosis-specific fates to ssDNA processing during homologous recombination. Understanding the functions of these RPA homologs and how they interact with the canonical RPA subunits is of major interest in the fields of meiosis and DNA repair. PMID:26520106

  7. Katariina kirikus algab Geeniuste mäss / Kai Ilustrumm

    Index Scriptorium Estoniae

    Ilustrumm, Kai

    2004-01-01

    Briti Nõukogu toetusel Pärnus, Tartus ja Tallinnas toimuvatel inglise filmide päevadel "Geeniuste mäss" näidatakse ka 3 Lindsay Andersoni nn. Travise triloogia filmi "Kui..." (1968), "Oo, õnneseen!" (1973) ja "Ravila Britannia" (1982), kus Mick Travise rollis on Malcolm McDowell (1943). Lisatud kava

  8. Variações de cor e atividade de água em Agaricus blazei desidratado e armazenado em diferentes embalagens plásticas - DOI: 10.4025/actascitechnol.v27i1.1505

    Directory of Open Access Journals (Sweden)

    Gilberto Costa Braga

    2005-03-01

    Full Text Available Neste trabalho foi avaliado o efeito de diferentes embalagens plásticas na atividade de água, na cor e no desenvolvimento microbiano em A. blazei desidratado durante o armazenamento. Amostras de 50 g de cogumelos secos foram embaladas em polipropileno (60 µm, polietileno de baixa densidade (50 µm, laminado duplo de polipropileno bi-orientado (44 µm e laminado duplo de polipropileno biorientado metalizado (40 µm e armazenadas por 0, 45, 90, 135, 180, 225 e 270 dias a 25°C e 80%UR. Análises microbiológicas de bolores e leveduras foram realizadas antes e após a armazenagem. A embalagem que promoveu maiores aumentos na atividade de água e no escurecimento dos basidiomas foi a de polipropileno, seguida das embalagens de polietileno de baixa densidade e de polipropileno bi-orientado duplo. A embalagem de polipropileno biorientado metalizado duplo proporcionou retenção de cor e não houve aumentos no desenvolvimento de bolores e leveduras nos cogumelos, durante o armazenamento, em nenhuma das embalagens plásticas estudadas

  9. 姬松茸粗多糖对K562细胞增殖、凋亡的影响及其作用机制探讨%Explore of the effects of Agaricus blazei polysaccharide on proliferation and apoptosis of cells K562 and its mechanism

    Institute of Scientific and Technical Information of China (English)

    林祥伟; 张苏伟

    2015-01-01

    目的:研究姬松茸粗多糖在K562细胞的增殖、凋亡,以及对转录因子NF-κB的影响,并探究其中的作用机制。方法培养K562细胞,再分别将(2、5、10mg/mL)姬松茸粗多糖加入其中作用48h,之后应用流式细胞术、免疫荧光法等方法观察姬松茸粗多糖在K562细胞的增殖、凋亡和对NF-κB活性中的影响。结果姬松茸粗多糖对K562细胞增殖有很显著的抑制作用,还可以阻止细胞从G0/G1期到G2/M+S期过渡。经过10mg/mL的姬松茸粗多糖诱导48h后,K562细胞CML细胞的凋亡率65.41%,较对照组显著增加,差异有统计学意义(P<0.01)。而且NF-κB较对照组减少。结论姬松茸粗多糖能抑制K562细胞增殖,并且可以诱导其凋亡,其中的作用机制和NF-κB通道的异常激活明显相关,值得进一步的研究。%Objective To study the influence of Agaricus blazei on proliferation and apoptosis of cells K562 and transcription NF- κB, and to explore the mechanism.Methods Cells K562 were cultured, then the 2, 5,10mg/mL of Agaricus blazei polysaccharides were respectively added to react for 48 hours, and then the effects of Agaricus blazei on proliferation and apoptosis of cells K562 and activity of NF- κB were observed with the methods such as flow cytometry, immunofluorescence.Results Agaricus blazei significantly inhibited the proliferation of cells K562, and also could prevent the cell transition from phase G0/G1 to phase G2/M+S. After induction by 10mg/mL of Agaricus blazei for 48 hours, apoptosis rate of cells K562 was 65.41%, which was significantly higher than that in control groups, and the differences were statistically significant (P<0.01). However there were fewer factors of NF-κB than the control groups.Conclusion Agaricus blazei can inhibit the proliferation of cells K562, and can induce apoptosis of them, the mechanism of that is obviously related with abnormal activation of channel of factors NF- κ

  10. Experimental atomic scale investigation of irradiation effects in CW 316SS and UFG-CW 316SS

    Science.gov (United States)

    Pareige, P.; Etienne, A.; Radiguet, B.

    2009-06-01

    Materials of the core internals of pressurized water reactor (austenitic stainless steels) are subject to neutron irradiation. To understand the ageing mechanisms associated with irradiation and propose life predictions of components or develop new materials, irradiation damage needs to be experimentally investigated. Atomic scale investigation of a neutron-irradiated CW316 SS with the laser pulsed atom probe gives a detailed description of the solute segregation in the austenitic grains. In order to understand the mechanism of solute segregation detected in the neutron-irradiated materials, ion irradiations were performed. These latest irradiations were realized on a CW 316SS as well as on a nanostructured CW 316SS. The study of irradiation effects in a nanograin material allows first, to easily analyse grain boundary segregation and second, to test the behaviour under irradiation of a new nanostructured material. The three aspects of this atomic scale investigation (neutron irradiation effect, model ion irradiation, new nanostructured CW 316 SS) are tackled in this paper.

  11. Vuorotyössä jaksaminen kliinisessä laboratoriossa

    OpenAIRE

    Kurtti, Elina; Niemi, Saija

    2014-01-01

    Opinnäytetyön tarkoituksena oli tutkia Vaasan keskussairaalan kliinisen laboratorion henkilökunnan vuorotyössä jaksamista. Opinnäytetyön tavoitteena oli selvittää kliiniselle laboratoriolle sen työntekijöiden jaksamista ja sitä, ovatko työntekijät tyytyväisiä työaikoihinsa ja olisiko niissä parannettavaa. Aihe saatiin Vaasan keskussairaalasta. Opinnäytetyössä tutkittiin, kuinka henkilökunta jaksaa vuorotyötä ja millaisia keinoja henkilökunta käyttää, jotta jaksaisi paremmin vuorotyötä. Opinnä...

  12. Optical results of the black hole microquasar SS 433

    Institute of Scientific and Technical Information of China (English)

    2010-01-01

    We present optical spectroscopic observations of SS 433 during different precessional and orbital phases.Our 2004,2007 and 2008 data provide us a good chance to study the variability of the Hα line at different orbital phases for nearly the same disk inclination.The data show that the Hα of SS 433 usually had a symmetric profile between orbital phases 0.25-0.75,while an asymmetric structure with a strong red peak was observed for other orbital phases.We suggest that the orbital variability of Hα emission is connected with accretion flow from the donor star to the accretion disk.In addition,we attribute the dramatic increase of Hα emission during our 2007 observational run,which had a time scale of one day,to the emergence of strong jets.

  13. Varhaisen tuen toimintamalli Ahlsell Oy:ssä

    OpenAIRE

    Vihtonen, Anne-Mari

    2012-01-01

    Varhaisen tuen toimintamalli Ahlsell Oy:ssä Vuosi 2012 Sivumäärä 51 Opinnäytetyöni tarkoituksena on selvittää mitä on hyvä johtaminen ja miten se vaikuttaa työ-hyvinvointiin. Työhyvinvointi on paljon puhuttu asia eikä suotta, koska siihen pitää kiinnittää erityistä huomiota, jotta henkilöstö jaksaisi työssään aina eläkeikään asti. Opinnäytetyöni tavoitteena on luoda toimintamalli, jota esimiehet ja johtajat voivat käyttää toimintansa tuk...

  14. Esimiehen juridinen rooli työyhteisössä

    OpenAIRE

    Hurme, Taina

    2015-01-01

    Opinnäytetyön toimeksiantajana oli OP-ryhmään kuuluva Osuuspankki. Opinnäytetyön aiheena oli tutkia esimiehen juridista roolia työyhteisössä. Tutkimuksen tarkoituksena oli selvittää mitä esimiehen oikeuksiin ja velvollisuuksiin kuuluu työlainsäädännön näkökulmasta ja miten hyvin esimiehet käytännössä tietävät mitä heidän juridiseen rooliinsa sisältyy. Lopputuloksen perusteella selviää myös onko organisaatiolla tarvetta heidän lisäkoulutukseensa ja pitääkö sitä kehittää tulevaisuudessa. Op...

  15. Taustatutkimus suunnittelutyössä - Pakki-opiskelijaportaalin konseptisuunnitelma

    OpenAIRE

    Ryynänen, Jenni

    2015-01-01

    Opinnäytetyöni aiheena oli toteuttaa Karelia-ammattikorkeakoulun Pakkiopiskelijaportaalin käyttäjille käyttäjätutkimus, jonka tulosten perusteella konseptisuunnitelma. Tavoitteisiin kuului myös suunnitella Pakki-portaalille käyttöliittymä, joka on käytettävyydeltään selkeä ja joka vastasi käyttäjien vaatimuksia ja toiveita. Opinnäyteyössäni perehdyn käyttäjälähtöisen suunnittelun periaatteisiin. Työssä pohditaan, mitä on käytettävyys sekä käyttökokemus ja kuinka käyttäjä otetaan huomioon ...

  16. Fluence dependence of deuterium retention in oxidized SS-316

    Science.gov (United States)

    Oya, Yasuhisa; Suzuki, Sachiko; Matsuyama, Masao; Hayashi, Takumi; Yamanishi, Toshihiko; Asakura, Yamato; Okuno, Kenji

    2011-10-01

    The ion fluence dependence of deuterium retention in SS-316 during oxidation at a temperature of 673 K was studied to evaluate the dynamics of deuterium retention in the oxide layer of SS-316. The correlation between the chemical state of stainless steel and deuterium retention was evaluated using XPS and TDS. It was found that the major deuterium desorption temperatures were located at around 660 K and 935 K, which correspond to the desorption of deuterium trapped as hydroxide. The deuterium retention increased with increasing deuterium ion fluence, since the deuterium retention as hydroxide increased significantly. However, retention saturated at an ion fluence of ˜2.5 × 10 21 D + m -2. The XPS result showed that FeOOD was formed on the surface, although pure Fe also remained in the oxide layer. These facts indicate the nature of the oxide layer have a key role in deuterium trapping behavior.

  17. Kumin kierrätys Teknikum Oy:ssä

    OpenAIRE

    Aaltonen, Katri

    2012-01-01

    Tämän työn tarkoituksena oli selvittää eri kierrätysmahdollisuuksia Teknikum Oy:ssä syntyvälle kumijätteelle. Alun perin työn tarkoituksena oli selvittää, onko yrityksen jätekumia mahdollista käyttää Valkeakoskella sijaitsevan Peatec Oy:n pyrolyysireaktorin raaka-aineena. Työn edetessä otettiin tarkastelun alle myös muita yrityksessä käynnissä olevia projekteja perustuen kierrätyskumin käyttöön raaka-aineena. Työssä esitellään Kuusakoski Oy:n valmistama kumipuru materiaalina, sekä uuden yhtei...

  18. LoCuSS: Testing hydrostatic equilibrium in galaxy clusters

    Science.gov (United States)

    Smith, G. P.; Mazzotta, P.; Okabe, N.; Ziparo, F.; Mulroy, S. L.; Babul, A.; Finoguenov, A.; McCarthy, I. G.; Lieu, M.; Bahé, Y. M.; Bourdin, H.; Evrard, A. E.; Futamase, T.; Haines, C. P.; Jauzac, M.; Marrone, D. P.; Martino, R.; May, P. E.; Taylor, J. E.; Umetsu, K.

    2016-02-01

    We test the assumption of hydrostatic equilibrium in an X-ray luminosity selected sample of 50 galaxy clusters at 0.15 sub-4 per cent, and our hydrostatic measurements of the same achieve excellent agreement between XMM-Newton and Chandra. The mean ratio of X-ray to lensing mass for these 50 clusters is β_X= 0.95± 0.05, and for the 44 clusters also detected by Planck, the mean ratio of Planck mass estimate to LoCuSS lensing mass is β_P= 0.95± 0.04. Based on a careful like-for-like analysis, we find that LoCuSS, the Canadian Cluster Comparison Project, and Weighing the Giants agree on β_P ≃ 0.9-0.95 at 0.15 Planck cosmology results from the cosmic microwave background and galaxy cluster counts.

  19. Susceptibility to Stress Corrosion Cracking of 254SMO SS

    Directory of Open Access Journals (Sweden)

    De Micheli Lorenzo

    2002-01-01

    Full Text Available The susceptibility to stress corrosion cracking (SCC of solubilized and sensitized 254SMO SS was studied in sodium chloride, and sodium fluoride solutions at 80 °C and sulfuric acid solutions in presence of sodium chloride at 25 °C. The influence of salt concentration, pH values and the addition of thiosulfate was examined. The susceptibility to SCC was evaluated by Slow Strain Rate Tests (SSRT, at 1.5 x 10-6 s-1 strain rate. The behavior of 254SMO was compared to those of AISI 316L SS and Hastelloy C276. 254SMO showed an excellent resistance to SCC in all conditions, except in the more acidic solutions (pH <= 1 where, in the sensitized conditions, intergranular stress corrosion cracking occurred.

  20. Elastic-plastic analysis of the SS-3 tensile specimen

    Energy Technology Data Exchange (ETDEWEB)

    Majumdar, S. [Argonne National Lab., IL (United States)

    1998-09-01

    Tensile tests of most irradiated specimens of vanadium alloys are conducted using the miniature SS-3 specimen which is not ASTM approved. Detailed elastic-plastic finite element analysis of the specimen was conducted to show that, as long as the ultimate to yield strength ratio is less than or equal to 1.25 (which is satisfied by many irradiated materials), the stress-plastic strain curve obtained by using such a specimen is representative of the true material behavior.

  1. Self-adaptive OmpSs tasks in heterogeneous environments

    OpenAIRE

    Planas J.; Badia R.M.; Ayguade E.; Labarta J.

    2013-01-01

    As new heterogeneous systems and hardware accelerators appear, high performance computers can reach a higher level of computational power. Nevertheless, this does not come for free: the more heterogeneity the system presents, the more complex becomes the programming task in terms of resource management. OmpSs is a task-based programming model and framework focused on the runtime exploitation of parallelism from annotated sequential applications. This paper presents a set of extensions to this...

  2. Ryhmässä toimiminen ja sosiaaliset taidot joukkueurheilussa

    OpenAIRE

    Liekola, Harri; Siikonen, Jyrki

    2015-01-01

    TIIVISTELMÄ KYMENLAAKSON AMMATTIKORKEAKOULU Sosiaalialan koulutusohjelma LIEKOLA, HARRI SIIKONEN, JYRKI Ryhmässä toimiminen ja sosiaaliset taidot joukkueurheilussa Opinnäytetyö 46 sivua + 9 liitesivua Työn ohjaaja Tuija Suikkanen-Malin Toimeksiantaja Kouvolan Pallonlyöjät ry. Pohjois-Kymen liikuntayhdistys ry. Toukokuu 2015 Avainsanat: ryhmätoiminta, sosiaaliset taidot, junioriurheilu, joukkueurheilu, pesäpallo, jääkiekko Opinnäytetyön tar...

  3. Hyvä hallinto ammattikorkeakoulun opintotukilautakuntatyössä

    OpenAIRE

    Jaakonsaari, Taina

    2009-01-01

    Hyvä hallinto on jokaiselle kuuluva perustuslailla turvattu oikeus. Perustuslain 21 §:n mukaan jokaisella on oikeus saada asiansa käsitellyksi asianmukaisesti ja ilman aiheetonta viivytystä toimivaltaisessa viranomaisessa. Opintotuki on usealle opiskelijalle pääasiallinen toimeentulon lähde, joten sen maksaminen oikea-aikaisesti ja oikean suuruisena on opiskelijan toimeentulon kannalta ensiarvoisen tärkeää. Opinnäytetyössä käsiteltiin ammattikorkeakouluopiskelijoiden opintotukiasioide...

  4. North Pacific Transition Zone Thickness From SS-Precursors

    Science.gov (United States)

    Mayeau, T. M.; Wysession, M. E.; Aleqabi, G. I.

    2005-12-01

    The thickness variation of the mantle transition zone is one key to understanding mantle dynamics, in particular, as a possible indicator of lateral temperature variations at that depth. As a result of the Clapeyron slopes of the 410 and 660 km phase transitions, the transition zone is expected to thin in regions of high heat and thicken in cooler areas. Thus, these variations can be used to look for large scale mantle convection features, such as down going slabs and mantle plumes. The thickness of the transition zone can be effectively measured by the differential travel times of phases reflecting off the 410 km and 660 km discontinuities. Here we search for transition zone thickness variations using SS-precursors from the North Pacific recorded by the IRIS PASSCAL Florida-to-Edmonton (FLED) Array of broadband seismometers (and neighboring North American stations), which were operational from May 2001 to September 2002. Records are first rotated into transverse components, converted to displacements with the instrument responses removed, and then subdivided into 20 degree by 20 degree bins. The traces from earthquakes with strong SS arrivals were binned by the location of the SS bounce point, and the bins stacked into vespagrams using the MATLAB-based MATSEIS program. From the vespagrams we determine the S660S-S410S differential travel times for each stack, which yield measurements of transition zone thickness. We then compare thickness measurements from different events and with previously published maps of transition zone thickness to determine the robustness of our technique. This work was supported by an IRIS Undergraduate Summer Internship.

  5. Revisiting the HST Fine Guidance Sensor Parallax of SS Cygni

    Science.gov (United States)

    Harrison, Thomas E.; McArthur, Barbara

    2016-01-01

    Recently, our results for the HST FGS parallax of SS Cyg (Harrison et al. 2000) have been called into question due to a recent radio parallax for this object (Miller-Jones et al. 2013). We have re-analyzed the data for our FGS program on SS Cyg and derive a revised parallax of 7.3 +/- 0.2 mas (137 pc). This parallax is 17% larger than that originally published. We discuss the new analysis effort, and the reasons for the change in our result. We believe we have identified why the radio parallax (8.77 mas) disagrees with the FGS results: We find that if we allow for a roll of two degrees in the HST coordinate system, we obtain the discrepant VLBA radio parallax. It is impossible, however, for the error in the roll of HST to be more than a few tenths of a degree, or else the FGS would not lock-on to the program targets. This roll leads to the large, and erroneous VLBA parallax for SS Cyg. We note that the radio parallax for the black hole system V404 Cyg (Miller-Jones et al. 2009) is also much larger than the spectroscopic parallax of its secondary star. In addition, we closely examine the attempt by Nelan & Bond (2013) to reconcile the FGS data set with the radio result. We identify what we believe to be significant issues with their analysis. Finally, we present a quantitative abundance analysis for SS Cyg, RU Peg, and GK Per derived from moderate resolution K-band spectroscopy. We find severe carbon deficiencies (10 to 20% of the solar value) in the secondary stars of all three objects. We describe the Python program "kmoog" which generates synthetic spectra, and autonomously compares them to observational data.

  6. Microstructure characteristic of SS304/BNi-2/SS304 butt joint by high temperature brazing%SS304/BNi-2/SS304高温钎焊对接接头微观结构特征

    Institute of Scientific and Technical Information of China (English)

    竺国荣; 陈虎; 祝金丹; 巩建鸣

    2011-01-01

    通过试验手段研究了SS304/BNi-2/SS304钎焊对接接头微观结构特征,使用能谱分析结合合金三元相图研究了接头区域典型的微观结构形貌和特征相变化规律,讨论了降熔元素在接头等温凝固连接过程中所起的关键作用。结果表明,在钎焊温度时,B从液相钎料向固相母材的快速扩散控制了接头的等温凝固过程,也因此决定了接头的微观结构相特征;钎焊接头通常会存在4种典型的相特征区域;钎角质量对于整个钎焊接头性能有重要影响。%The microstructure characteristic of SS304/Bni -2/SS304 butt joint was analyzed by high temperature brazing through the experimental research. Some typical microstructural morphologies and phase transition feature of the butt joint were studied by using ternary phase diagrams and EDS analysis. The critical effect of element diffusion on isothermal solidification of brazed joint was also discussed by analyzing the microstructural evolution. The results showed that the quick diffusing of boron from the liquid filler alloy into solid base metal controlled the isothermal solidification process as well as the joint microstructure. Four typical characteristic zones could be observed for the whole brazed joint. The fillet quality played a very important role in the whole joint performance.

  7. Käsihygienia hoitotyössä

    OpenAIRE

    Viitala, Jenni; Hakala, Tiia

    2014-01-01

    Tutkimuksen tarkoituksena oli selvittää Vaasan keskussairaalan teho-osastojen ja kirurgisten vuodeosastojen hoitohenkilökunnan käsihygienian toteutumista hoitotyössä. Tutkimuksen tavoitteena on lisätä hoitohenkilökunnan tietoa oikeasta käsihygieniasta ja siten estää infektioiden leviämistä osastoilla. Tutkimusaineisto kerättiin strukturoidulla kyselylomakkeella Vaasan keskussairaalan aikuisten ja lasten teho-osastoilta ja kirurgisilta vuodeosastoilta. Vastausprosentti oli 81. Kyselylomake ...

  8. LoCuSS: Testing hydrostatic equilibrium in galaxy clusters

    CERN Document Server

    Smith, G P; Okabe, N; Ziparo, F; Mulroy, S L; Babul, A; Finoguenov, A; McCarthy, I G; Lieu, M; Bahe, Y; Bourdin, H; Evrard, A E; Futamase, T; Haines, C P; Jauzac, M; Marrone, D P; Martino, R; May, P E; Taylor, J E; Umetsu, K

    2015-01-01

    We test the assumption of hydrostatic equilibrium in an X-ray luminosity selected sample of 50 galaxy clusters at $0.15SS). Our weak-lensing measurements of $M_{500}$ control systematic biases to sub-4 per cent, and our hydrostatic measurements of the same achieve excellent agreement between XMM-Newton and Chandra. The mean ratio of X-ray to lensing mass for these 50 clusters is $\\beta_{\\rm X}=0.95\\pm0.05$, and for the 44 clusters also detected by Planck, the mean ratio of Planck mass estimate to LoCuSS lensing mass is $\\beta_{\\rm P}=0.95\\pm0.04$. Based on a careful like-for-like analysis, we find that LoCuSS, the Canadian Cluster Comparison Project (CCCP), and Weighing the Giants (WtG) agree on $\\beta_{\\rm P}\\simeq0.9-0.95$ at $0.15

  9. Tritium uptake by SS316 and its decontamination

    Science.gov (United States)

    Torikai, Y.; Penzhorn, R.-D.; Matsuyama, M.; Watanabe, K.

    2004-08-01

    As-received and highly polished SS316 specimens were loaded with HT at 473-573 K. The uptake by polished samples was found to be up to five times that of as-received ones, when loading was performed immediately after polishing. This disparity vanished when polished specimens were subjected to a prolonged exposure to air prior to loading. The tritium loss from tritium-loaded SS316 specimens was examined by chemical etching and by thermal release in a flow system using various carrier gases at several temperatures. While at moderate temperatures the type of carrier has an impact on the tritium release rate, at higher ones this effect disappears. Moisture in the carrier gas has little influence on the loss rate of bulk tritium. Etching depth profiles of specimens previously heat-treated in the presence of air or Ar + H 2 and of untreated specimens are given. Evidence for chronic tritium liberation from SS316 at 298 K is provided.

  10. Investigation on SS316, SS440C, and Titanium Alloy Grade-5 used as Single Point Cutting Tool

    Directory of Open Access Journals (Sweden)

    Mr.Kothakota Suresh Kumar

    2015-01-01

    Full Text Available The main objective of this work is to find alternative materials for the cutting tools used in turning operations. The conventional materials like tungsten carbide(WC, titanium carbide(TiC, cubic boron nitride (CBN and diamond used as cutting tools for turning operations on lathe are expensive. Titanium grade 5 (Ti-6Al-4V, SS440C/AISI440C and SS316 are some of the materials which satisfy the necessary requirements for turning metals and polymer materials. These materials are machined as per the standard tool signature of high-speed steel tool (HSS and are subjected to necessary heat treatment for hardening and then finish ground. The machined tools thus prepared were used to turn mild steel and aluminium workpieces. The cutting forces at play are determined using lathe tool dynamometer and plotted on a MCD (Merchant’s Circle Diagram. The cutting tools are also subjected to tests to determine tool life, wear and work hardening. It is found that the performance and tool life of SS440C is better and cost effective compared to existing tools. Even though Ti-6Al-4V is comparatively costly it could be used for obtaining good surface finish.

  11. Lapsen harjaantuminen englannin kielen käytössä päiväkotiympäristössä

    OpenAIRE

    Lahtinen, Hanna

    2013-01-01

    Opinnäytetyön aiheena on lapsen harjaantuminen englannin kielen käytössä päiväkotiympäristössä. Tavoitteena on tutkia lasten vieraan kielen oppimisen tukemista päiväkotiympäristössä, ja samalla selvittää, miten kotiympäristössä tuetaan englannin kielen käyttöä toisen vanhemman äidinkielen ollessa englanti. Työn tarkoituksena on tuoda esille käytettyjä menetelmiä ja keinoja englannin kielen opettamiseen ja kielen käytön tukemiseen. Kotkan Englanninkielinen leikkikoulu, varhaiskasvatussuunnitel...

  12. Polysaccharide Agaricus blazei Murill stimulates myeloid derived suppressor cell differentiation from M2 to M1 type, which mediates inhibition of tumour immune-evasion via the Toll-like receptor 2 pathway.

    Science.gov (United States)

    Liu, Yi; Zhang, Lingyun; Zhu, Xiangxiang; Wang, Yuehua; Liu, WenWei; Gong, Wei

    2015-11-01

    Gr-1(+) CD11b(+) myeloid-derived suppressor cells (MDSCs) accumulate in tumor-bearing animals and play a critical negative role during tumor immunotherapy. Strategies for inhibition of MDSCs are expected to improve cancer immunotherapy. Polysaccharide Agaricus blazei Murill (pAbM) has been found to have anti-cancer activity, but the underlying mechanism of this is poorly understood. Here, pAbM directly activated the purified MDSCs through inducing the expression of interleukin-6 (IL-6), IL-12, tumour necrosis factor and inducible nitric oxide synthase (iNOS), CD86, MHC II, and pSTAT1 of it, and only affected natural killer and T cells in the presence of Gr-1(+) CD11b(+) monocytic MDSCs. On further analysis, we demonstrated that pAbM could selectively block the Toll-like receptor 2 (TLR2) signal of Gr-1(+) CD11b(+) MDSCs and increased their M1-type macrophage characteristics, such as producing IL-12, lowering expression of Arginase 1 and increasing expression of iNOS. Extensive study showed that Gr-1(+) CD11b(+) MDSCs by pAbM treatment had less ability to convert the CD4(+) CD25(-) cells into CD4(+) CD25(+) phenotype. Moreover, result from selective depletion of specific cell populations in xenograft mice model suggested that the anti-tumour effect of pAbM was dependent on Gr-1(+ ) CD11b(+) monocytes, nether CD8(+) T cells nor CD4(+) T cells. In addition to, pAbM did not inhibit tumour growth in TLR2(-/-) mice. All together, these results suggested that pAbM, a natural product commonly used for cancer treatment, was a specific TLR2 agonist and had potent anti-tumour effects through the opposite of the suppressive function of Gr-1(+) CD11b(+) MDSCs. PMID:26194418

  13. The polar high molecular weight fraction of the Agaricus blazei Murill extract, AndoSan™, reduces the activity of the tumor-associated protease, legumain, in RAW 264.7 cells.

    Science.gov (United States)

    Berven, Lise; Karppinen, Pernille; Hetland, Geir; Samuelsen, Anne Berit C

    2015-04-01

    AndoSan™ is an extract of Agaricus blazei Murill (AbM; 82.4%), Hericium erinaceum (14.7%), and Grifola frondosa (2.9%). The main ingredient of AndoSan, AbM, is rich in different forms of β-glucans. Since these exhibit potent antitumor activity and have immunomodulatory effects, the stimulatory effect of AndoSan on the production of different cytokines, chemokines, and leukocyte growth factors has predominantly been attributed to β-glucans. AndoSan has been claimed to consist of 90% carbohydrate, of which 2.8% is β-glucans, but in this study, we show that the carbohydrate content is only 2% of the dry weight, corresponding to 0.09% β-glucan per mL of AndoSan. Fractionation of AndoSan, followed by carbohydrate analysis and HPLC analysis revealed that most of the glucose was concentrated in the polar high molecular weight fraction of AndoSan (ethanol insoluble water extract [EIWE]-A) and that this extract was able to significantly inhibit the activity of the tumor-associated protease, legumain, in RAW 264.7 cells. Legumain is synthesized as a zymogen and undergoes pH-dependent autoactivation of the proform to reach an enzymatically active form. In this study, we demonstrate that both the polar and nonpolar AndoSan fractions are able to inhibit the autoactivation of prolegumain, and that the polar fractions of AndoSan are the most potent inhibitors of the active form of the enzyme. PMID:25136950

  14. Medicinal Mushroom Growth as Affected by Non-Axenic Casing Soil

    Institute of Scientific and Technical Information of China (English)

    D. C. ZIED; M. T. A. MINHONI; J. KOPYTOWSKI-FILHO; L. BARBOSA; M. C. N. ANDRADE

    2011-01-01

    Ten different casing soils were collected from two soils at two depths (0.2 and 2.0 m below soil surface) to examine the relationships between the physical properties of non-axenic casing soil and yield, number and weight of the medicinal mushroom Agaricus blazei ss. Heinemann. The results showed that soil clay content and bulk density were negatively correlated with the mushroom yield,respectively, but soil silt content and water-holding capacity were found to be positively correlated with the yield. The number of mushrooms was negatively correlated with soil water-holding capacity but positively correlated with soil clay, bulk density and porosity.The weight of mushroom was positively correlated with the content of soil fine sand and negatively correlated with the contents of soil coarse sand, total sand and clay. Neither soil depth nor different soil combinations affected the yield and number of mushrooms, but the mushroom weight was affected by the soil combinations and soil depth, so interplay in the fructification process with the physical characteristics of casing is complicated.

  15. Wapice IoT-Ticket.com -valvomoalusta kiinteistöautomaatiokäytössä

    OpenAIRE

    Vehmaanperä, Joonas

    2016-01-01

    ssä opinnäytetyössä tutkittiin Wapice Oy:n IoT-Ticket.com -valvomoalustan soveltuvuutta kiinteistöautomaation valvontaan ja Enermix Oy:n tarpeisiin. Työssä käsiteltiin IoT-Ticket.com -alustan ominaisuuksia ja toimintoja vertailemalla niitä nykyisin Enermix Oy:n käytössä olevaan valvomoalustaan. Vertailutasona käytettiin nykyisellä valvomoalustalla toteutettua poistoilman lämmöntalteenotto kohteen valvomoa. Opinnäytetyössä tärkeimpinä vertailtavia seikkoja valvomoalustojen välillä olivat työ...

  16. QR-koodi turvallisuusviestinnässä

    OpenAIRE

    Käkönen, Valtteri

    2016-01-01

    Tämän päättötyön aiheena oli kartoittaa QR-koodin käyttöä ja käyttömahdollisuuksia turvallisuusviestinnässä. QR-koodi on lyhenne sanoista Quick Response ja tämä tekniikka on lähtöisin tehtaista, joissa liukuhihnalla kulkevien tavaroiden nopea tunnistaminen on tarpeellista. QR-koodin voi lukea kamerallisella mobiililaitteella. Yleensä QR-koodi luodaan internetsivusta, jolloin käyttäjä on helppo ohjata halutun aineiston ääreen URL-osoitteen pituudesta riippumatta. Selvitys aloitettiin etsim...

  17. Verkon laitteiden valvonta teollisuusympäristössä

    OpenAIRE

    Taskila, Antti

    2012-01-01

    Tämä on Ruukki Oy:n Raahen tehtaalle toteutettu opinnäytetyö, jonka tavoite oli luoda ohjelma ja valvoa siihen lisättyjen laitteiden saavutettavuutta verkossa. Aiemmin tiedot toimimattomista laitteista kulkivat useamman välikäden kautta esimerkiksi työntekijältä työnjohtajalle, joka taas ottaa yhteyttä parhaakseen katsomaansa henkilöön. Tässä ketjussa saattaa kestää pitkiäkin aikoja. Tästä johtuva tauko saattaa laskea tuotannon laitteiden toimintatehoa suurestikin. Aluksi mietittiin eri t...

  18. Osaaminen monipalvelutyössä ruoka- ja puhdistuspalvelualalla

    OpenAIRE

    Enqvist, Annika

    2015-01-01

    Opinnäytetyön tavoitteena oli selvittää ruoka- ja puhdistuspalvelualan monipalvelutyön osaamisvaatimuksia kunta-alalla. Lisäksi selvitettiin, miten työntekijä voi hankkia monipalvelutyössä tarvittavan osaamisen. Tutkimusote oli kvalitatiivinen, tutkimusmenetelmä teemahaastattelu ja sähköpostikysely. Haastatteluihin osallistui yksitoista ruoka- ja puhdistuspalvelualan asiantuntijaa, ja viisi asiantuntijaa vastasi sähköpostitse. Vastaukset jaoteltiin kolmeen osaamispääomaluokkaan: henkilö-,...

  19. Pilvipalvelut yrityskäytössä

    OpenAIRE

    Farsi Madan, Alex Mehdi

    2016-01-01

    Insinöörityössä perehdyttiin pilvipalveluihin ja siihen, miksi niiden suosio on noussut viime vuosien aikana. Tavoitteena oli selvittää, mitä toimenpiteitä kohdeyrityksen täytyy tehdä Google Apps for Workin käyttöönotossa. Google Apps for Work on Googlen yrityksille tarjoama palvelupaketti, joka sisältää web-pohjaisia viestintä- ja yhteistyösovelluksia. Opinnäytetyön tavoitteena oli vertailla puolueettomasti kahta yhteistyösovellusten markkinajohtajaa Googlea ja Microsoftia. Lisäksi tavo...

  20. Mielenterveyskuntoutujien ruokailutottumusten ‎kartoitus EMY ry:ssä

    OpenAIRE

    Luukkonen, Anni; Isokangas, Anna-Liisa

    2010-01-01

    Opinnäytetyömme tarkoituksena on kartoittaa mielenterveyskuntoutujien näkemyksiä terveellisestä ‎ruoasta, heidän omia ruokailutottumuksiaan ja näkemyksiä talkooruokailusta Espoon ‎mielenterveysyhdistys ry:ssä (EMY ry). EMY ry tekee töitä mielenterveyden edistämiseksi Espoon ‎seudulla. EMY ry on esittänyt Laurea-ammattikorkeakoulun hankefoorumipäivillä tutkimusideansa ‎talkooruokailunsa kehittämisestä. Toteutimme opinnäytetyön osana Työn rajalla- hanketta, joka ‎on osa Laurea-ammattikorkeakoul...

  1. Interaktiiviset valkotaulut opetuskäytössä

    OpenAIRE

    Pääkkönen, Tero

    2010-01-01

    Tämän työn tarkoituksena oli kuvata interaktiivisten valkotaulujen käyttöä ja ominaisuuksia opetuskäytössä. Interaktiiviseksi valkotauluksi kutsutaan järjestelmää, jossa on tyypillisesti yhdistettynä tietokone, dataprojektori ja valkotaulu liikkeentunnistustekniikalla. Tällöin laitteisto asennetaan paikalleen kiinteästi. Interaktiivisen valkotaulun toimintaperiaatteena on heijastaa tietokoneen näyttö valkotaululle, joka toimii suurena kosketusnäyttönä. Valkotaulun käytetyimmät liikkeentunnist...

  2. Materiaalivirtojen tehostaminen Mac Steel Oy:ssä

    OpenAIRE

    Toivonen, Mika

    2011-01-01

    Työssä tutkitaan Mac Steel Oy:n materiaalivirta ongelmia, ongelman lähteitä ja keinoja päästä ongelmista eroon. Mac Steelin tuotanto on keskitytty suuriin kappaleisiin, jotka aiheuttavat tietynlaisia ongelmia materiaalien ja valmiiden tuotteiden käsittelyssä. Asiakaskunta on laaja johon kuuluu maamme johtavia alumiiniveneiden ja ajoneuvoteollisuuden yrityksiä, lisäksi tuotteita valmistetaan teräsrakenne teollisuuden tarpeisiin. Lähtökohtana on tutkia Lean ajatusmaailmaa ja sieltä löytää ne...

  3. Grafiikan ohjaus Vizrt-järjestelmässä

    OpenAIRE

    Makkonen, Jani

    2013-01-01

    Ensin työssä esitellään Vizrt-järjestelmän eri osat eli Content Pilot, Viz Artist, Viz Template Wizards ja Viz Engine. Content Pilot on sapluunoiden käyttämiseen tarkoitettu sovellus. Viz Artist taas on grafiikan tekemiseen tarkoitettu sovellus. Template Wizardilla tehdään sapluunoita ja Viz Enginessä toistetaan valmiit scenet eli näytetään grafiikka. Sitten käydään läpi mitä grafiikan ohjaaminen tarkoittaa, kerrotaan mitä scenet ja sapluunat ovat ja kerrotaan mitä ovat sapluunoiden v...

  4. Pukusuunnittelu näytelmässä Transfinlandia

    OpenAIRE

    Syrjäkari, Tellervo

    2014-01-01

    Lopputyöni taiteellinen osuus on pukusuunnittelu Paula Vesalan kirjoittamaan näytelmään Transfinlandia, jonka kantaesitys sai ensi-iltansa Mediakeskus Lumen studionäyttämöllä 10.4.2014. Näytelmän ohjasi Piia Peltola. Lopputyöni kirjallinen osuus nojaa kokonaisuudessaan taiteelliseen työskentelyyni kyseisessä näytelmässä. Näytelmä kertoo tarinan sukupuolenkorjausprosessia läpikäyvästä pojasta, josta tulee nainen. Olen rajannut kirjallisen työni käsittelemään taiteellista pukusuunnittelut...

  5. Psykiatriset potilastapaukset simulaatio-oppimisympäristössä

    OpenAIRE

    Manssila, Marko

    2013-01-01

    Tämän opinnäytetyön tarkoituksena oli kehittää simulaatiokoulutusta opiskelijoiden oppimisympäristönä ja luoda siihen erilaisia psykiatrisia potilastapauksia. Tämä opinnäytetyö perustui Laurea-ammattikorkeakoulun Lohjan toimipisteen sosiaali- ja terveysalan simulaatio-oppimisympäristön kehittämishankkeeseen. Kehittämishankkeessa oli tarkoituksena luoda somaattisia ja psykiatrisia potilastapauksia, joita käytetään koulutuksessa simulaatio-oppimisympäristössä hyödyntäen simulaatio-ohjelmist...

  6. KOTI KIIKARISSA : Kokemusasiantuntijuus maahanmuuttajien asumiskoulutustyössä

    OpenAIRE

    Haltia, Eero

    2012-01-01

    TIIVISTELMÄ Haltia, Eero. Koti Kiikarissa - Kokemusasiantuntijuus maahanmuuttajien asumiskoulutustyössä. Järvenpää, syksy 2012, 70s., 4 liitettä. Diakonia-ammattikorkeakoulu, Järvenpään yksikkö, Sosiaalialan koulutusohjelma, Diakonian suuntautumisvaihtoehto, sosionomi - diakoni (AMK) Kehittämishankkeen tarkoituksena on julkistaa kokemuksia maahanmuuttajan kokemusasiantuntijuuden käytöstä heidän asumiskoulutuksessaan. Toimintaympäristönä on Espoon kaupunki ja Espoon Järjestöjen Yhteis...

  7. Kotouttava ja ohjaava ote pakolaisperhetyössä

    OpenAIRE

    Toom, Nita; Vesterinen, Tanja

    2012-01-01

    Tiivistelmä Opinnäytetyön tarkoituksena oli tutkia Jyväskylän maahanmuuttajapalveluiden asiakkaana olevien pakolaisperheiden kanssa tehtävää lastensuojelullista perhetyötä. Toimeksiantajanamme toimi Jyväskylän kaupungin maahanmuuttajapalvelut. Tavoitteena oli selvittää millaista perhetyötä pakolaisperheiden kanssa tehdään, mikä tukee perhetyön onnistumista ja mitä erityispiirteitä ja haasteita perhetyön toteutumiseen liittyy. Pyrimme myös selvittämään, miten perhetyössä näkyy perheen kot...

  8. Musiikki ja musiikkiterapia vaihtoehtoisena hoitomuotona hoitotyössä

    OpenAIRE

    Pelho, Veera

    2013-01-01

    Musiikilla on ihmiseen monimuotoisia vaikutuksia sekä fyysisesti että psyykkisesti. Sitä on käytetty universaalisesti jo vuosisatojen ajan apuna ihmiselämän muutoksissa. Tämä opinnäytetyö käsittelee musiikin ja musiikkiterapian käyttöä somaattisesti sairaiden potilaiden parissa. Opinnäytetyön tarkoitus on selvittää voiko musiikki ja musiikkiterapia olla osa hoitotyötä, voiko siitä olla apua hoitotyössä sekä tarkastella millaisia hoitotuloksia musiikilla ja musiikkiterapialla on saatu. ...

  9. Hiljaisen tiedon mallittaminen Suominen Kuitukankaat Oy:ssä

    OpenAIRE

    Järvistö, Tero

    2007-01-01

    Tämän opinnäytetyön aiheena on hiljaisen tiedon mallittaminen Suominen Kuitukankaat Oy:ssä. Tutkimuksen tarkoituksena oli löytää keino yrityksen avainhenkilöiden hiljaisen tiedon siirtämiseen mahdollisimman eksplisiittiseen muotoon. Tavoitteena oli luoda yritykselle konkreettinen käytäntö, jonka avulla se voi pyrkiä siirtämään hiljaista tietoa dokumentoituun muotoon. Työn teoriaosassa esitetään hiljaisen tiedon määritelmä sekä käsitellään sen muuttamista eksplisiittiseen muotoon. Lisäksi pohd...

  10. Mobiililaitteiden hallinta Windows -palvelinympäristössä

    OpenAIRE

    Hänninen, Joonas

    2014-01-01

    Mobiililaitteet ovat yleistyneet valtavasti muutaman viime vuoden aikana. Organisaatioiden työntekijöiden ei enää tarvitse istua kiinteän työpisteen ääressä. Tämä asettaa uusia haasteita organisaation järjestelmänylläpidolle ja tietoturvalle. Mobiililaitteet pitäisi saada hallittavaksi, kuten perinteiset tietokoneet. On olemassa erilaisia vaihtoehtoja, joita organisaatio voi toteuttaa. Opinnäytetyössä tutustutaan yhteen ratkaisuun ja selvitetään, miten laitteiden hallinta onnistuu. Selvit...

  11. Stress analysis around DSCu/SS316 HIP bonded interface

    International Nuclear Information System (INIS)

    HIP bonded structure of DSCu/SS316 is proposed for ITER plasma facing components. In 20th SOFT, we reported the fracture strength of HIP bonded interface at ambient temperature. But first wall is at high temperature in operation, we estimate in this study the fracture strength of HIP bonded interface at 473K as a first step of high temperature. The results of fracture strength test indicate that the fracture strength of HIP bonded interface at high temperature is lower than that at ambient temperature. The fracture surfaces of specimens ruptured at ambient temperature and at 473K were observed. Crack surfaces were nearly 10 μm apart from the bonded interface into DSCu side. Scanning electron microscope observation is considered to show the existence of the thin new layer in DSCu region in which the dispersed Al2O3 was diminished. Assuming the thin layer to be composed of Cu, fracture behavior of HIP bonded structure is simulated by three layers model DSCu/Cu/SS316 and the fracture strength is estimated by the stress intensity factor K. The analytical results conclude that crack propagates in DSCu/Cu interface more easily, which is good agreement with fracture surface in experiments

  12. Acousto-microfluidics for screening of ssDNA aptamer

    Science.gov (United States)

    Park, Jee-Woong; Lee, Su Jin; Ren, Shuo; Lee, Sangwook; Kim, Soyoun; Laurell, Thomas

    2016-01-01

    We demonstrate a new screening method for obtaining a prostate-specific antigen (PSA) binding aptamer based on an acoustofluidic separation (acoustophoreis) technique. Since acoustophoresis provides simultaneous washing and separation in a continuous flow mode, we efficiently obtained a PSA binding aptamer that shows high affinity without any additional washing step, which is necessary in other screening methods. In addition, next-generation sequencing (NGS) was applied to accelerate the identification of the screened ssDNA pool, improving the selecting process of the aptamer candidate based on the frequency ranking of the sequences. After the 8th round of the acoustophoretic systematic evolution of ligands by exponential enrichment (SELEX) and following sequence analysis with NGS, 7 PSA binding ssDNA aptamer-candidates were obtained and characterized with surface plasmon resonance (SPR) for affinity and specificity. As a result of the new SELEX method with PSA as the model target protein, the best PSA binding aptamer showed specific binding to PSA with a dissociation constant (Kd) of 0.7 nM. PMID:27272884

  13. Acousto-microfluidics for screening of ssDNA aptamer.

    Science.gov (United States)

    Park, Jee-Woong; Lee, Su Jin; Ren, Shuo; Lee, Sangwook; Kim, Soyoun; Laurell, Thomas

    2016-01-01

    We demonstrate a new screening method for obtaining a prostate-specific antigen (PSA) binding aptamer based on an acoustofluidic separation (acoustophoreis) technique. Since acoustophoresis provides simultaneous washing and separation in a continuous flow mode, we efficiently obtained a PSA binding aptamer that shows high affinity without any additional washing step, which is necessary in other screening methods. In addition, next-generation sequencing (NGS) was applied to accelerate the identification of the screened ssDNA pool, improving the selecting process of the aptamer candidate based on the frequency ranking of the sequences. After the 8(th) round of the acoustophoretic systematic evolution of ligands by exponential enrichment (SELEX) and following sequence analysis with NGS, 7 PSA binding ssDNA aptamer-candidates were obtained and characterized with surface plasmon resonance (SPR) for affinity and specificity. As a result of the new SELEX method with PSA as the model target protein, the best PSA binding aptamer showed specific binding to PSA with a dissociation constant (Kd) of 0.7 nM. PMID:27272884

  14. Johtamisen työkalut vapaaehtoistyössä : Viestintä Tampereen Teatterin Naisyhdistys RY:ssä

    OpenAIRE

    Mäntylä, Mirja

    2013-01-01

    Opinnäytetyö on tehty Tampereen Teatterin Naisyhdistys RY:lle ja Tampereen Teatteri Oy:lle. Tampereen Teatterin Naisyhdistys RY organisoi vapaaehtoistyötä kulttuurin hyväksi. Yhdistys toimii Tampereen Teatterissa miehittämällä Tampereen Teatterin palvelut Oy:n kahvilatoiminnan työvuoroja teatterinäytösten aikaisessa kahvilamyynnissä. Opinnäytetyön tarkoituksena on tutkia johtamisen työkalua, viestintää, Tampereen Teatterin Naisyhdistys RY:ssä toimivien ryhmäpäälliköiden näkökulmasta. Nykytila...

  15. Y-sukupolven johtaminen Airpro Oy:ssä

    OpenAIRE

    Lehtola, Heikki

    2014-01-01

    Y-sukupolvi on jo hämmentämässä työelämää. Näitä 1980−1990 -luvuilla syntyneitä ihmisiä kuvaillaan kärsimättömäksi sukupolveksi, joka ei auktoriteetteja kunnioita. Monissa yrityksissä ollaan hieman hämillään näiden nuorien työntekijöiden kanssa, sillä heitä tuntuvat motivoivan erilaiset asiat kuin aikaisempia sukupolvia. Airpro Oy:n turvatarkastusyksikkö muodostaa yrityksen liikevaihdollisesti suurimman yksikön. Sen 336 työntekijästä suurin osa kuuluu y-sukupolveen. Yrityksessä on huomatt...

  16. Quiescent and outburst photometry of the dwarf nova SS Cygni

    Energy Technology Data Exchange (ETDEWEB)

    Honey, W.B.; Bath, G.T.; Charles, P.A. and others

    1989-02-01

    It has been suggested that the wide outbursts of U Gem stars are analogous to the wide or 'super' outbursts of SU UMa stars and hence that the basic mechanism which gives rise to the two types of outburst is the same. If this is true, then the U Gem type stars should also exhibit some of the more exotic properties associated with superoutbursts in SU UMa stars, in particular superhumps. In order to test this hypothesis, extensive photometric observations of a wide and a narrow outburst (and also the period of quiescence separating them) of the dwarf nova SS Cygni were obtained in order to search for modulations at or near the orbital period. No such modulations were found in either the wide or narrow outbursts to a limit of /similar to/ 0.05 mag.

  17. Interaction of deuterium with SS316 austenitic stainless steel

    International Nuclear Information System (INIS)

    Accumulation and desorption of deuterium implanted in SS316 austenitic stainless steel to concentrations ∼1 at.%, the influence of helium and radiation-induced defects on the process of mass transfer of deuterium and the mechanical properties of steel at different levels of damage were studied. The samples were irradiated with 15 keV/D, 30 keV/He and 1.4 MeV/Ar ions. For modeling of the defect structure formed in the materials of nuclear power plants, the irradiation with high-energy argon ions was performed. Studies were carried out by means of ion implantation, nuclear reactions D(3He,p)4He with analyzing beam of 3He (E = 0.3...1.4 MeV), thermal desorption spectroscopy, transmission electron microscopy and nanoindentation. It was found that the retention of deuterium in steel is increased significantly in the presence of radiation damage created by helium and argon pre-implantation

  18. PCI-SS: MISO dynamic nonlinear protein secondary structure prediction

    Directory of Open Access Journals (Sweden)

    Aboul-Magd Mohammed O

    2009-07-01

    Full Text Available Abstract Background Since the function of a protein is largely dictated by its three dimensional configuration, determining a protein's structure is of fundamental importance to biology. Here we report on a novel approach to determining the one dimensional secondary structure of proteins (distinguishing α-helices, β-strands, and non-regular structures from primary sequence data which makes use of Parallel Cascade Identification (PCI, a powerful technique from the field of nonlinear system identification. Results Using PSI-BLAST divergent evolutionary profiles as input data, dynamic nonlinear systems are built through a black-box approach to model the process of protein folding. Genetic algorithms (GAs are applied in order to optimize the architectural parameters of the PCI models. The three-state prediction problem is broken down into a combination of three binary sub-problems and protein structure classifiers are built using 2 layers of PCI classifiers. Careful construction of the optimization, training, and test datasets ensures that no homology exists between any training and testing data. A detailed comparison between PCI and 9 contemporary methods is provided over a set of 125 new protein chains guaranteed to be dissimilar to all training data. Unlike other secondary structure prediction methods, here a web service is developed to provide both human- and machine-readable interfaces to PCI-based protein secondary structure prediction. This server, called PCI-SS, is available at http://bioinf.sce.carleton.ca/PCISS. In addition to a dynamic PHP-generated web interface for humans, a Simple Object Access Protocol (SOAP interface is added to permit invocation of the PCI-SS service remotely. This machine-readable interface facilitates incorporation of PCI-SS into multi-faceted systems biology analysis pipelines requiring protein secondary structure information, and greatly simplifies high-throughput analyses. XML is used to represent the input

  19. GSM-moduuli sulautetussa järjestelmässä

    OpenAIRE

    Rantanen, Timo

    2011-01-01

    ssä opinnäytetyössä tutustuttiin GSM-moduulin ohjaukseen ja käyttöön sulautetussa järjestelmässä. Työssä käytettiin apuna Olimexin AVR-GSM-kehitysalustaa. Kehitysalustan tärkeimmät komponentit ovat ATMega32-mikrokontrolleri sekä Simcomin SIM300D GSM-moduuli. Työssä esitellään AVR-GSM-kehitysalusta ja SIM300D GSM-moduulin ohjaus AT-komennoilla. Työssä tehtiin myös esimerkkisovellus, joka havainnollistaa kehitysalustan ja GSM-moduulin käyttöä ja ohjausta. Opinnäytetyön ohjelmistokehitys t...

  20. Effect of the Medicinal Agaricus blazei Murill-Based Mushroom Extract, AndoSanTM, on Symptoms, Fatigue and Quality of Life in Patients with Crohn’s Disease in a Randomized Single-Blinded Placebo Controlled Study

    Science.gov (United States)

    Hetland, Geir; Lyberg, Torstein; Lygren, Idar; Johnson, Egil

    2016-01-01

    Background Ingestion of AndoSanTM, based on the mushroom Agaricus blazei Murill, has previously shown an anti-inflammatory effect through reduction of pro-inflammatory cytokines in healthy individuals and patients with Crohn’s disease (CD). In this randomized single-blinded placebo-controlled study we examined whether intake of AndoSanTM also resulted in clinical effects. Methods and Findings 50 patients with symptomatic CD were randomized for oral daily consumption of AndoSanTM or placebo for a 21-day experimental period, in this per-protocol study. Patients reported validated scores for symptoms, fatigue and health related quality of life (HRQoL) at days 0, 14 and 21. Fecal calprotectin and general blood parameters were also analyzed. In the AndoSanTM group (n = 25) symptoms improved from baseline (day 0) to days 14 and 21, with respective mean scores (95% CI) of 5.52 (4.64–6.40), 4.48 (3.69–5.27) and 4.08 (3.22–4.94) (p<0,001). We found significant improvements in symptom score for both genders in the AndoSanTM group, and no significant changes in the placebo (n = 25) group. There were however no significant differences between the groups (p = 0.106), although a marginal effect in symptom score for men (p = 0.054). There were comparable improvements in physical, mental and total fatigue for both groups. HRQoL versus baseline were at day 21 improved for bodily pain and vitality in the AndoSanTM group and for vitality and social functioning in the placebo group. No crucial changes in general blood samples and fecal calprotectin were detected. Conclusions The results from this single-blinded randomized clinical trial shows significant improvement on symptoms, for both genders, in the AndoSanTM group, but no significant differences between the study groups. The results on fatigue, HRQoL, fecal calprotectin and blood samples were quite similar compared with placebo. The patients did not report any harms or unintended effects of AndoSanTM. CD patients with

  1. "Hyväksyvässä ilmapiirissä kannattaisi kaikkien ainakin vierailla" : ryhmätoiminnan merkitys mielenterveyskuntoutujien elämässä

    OpenAIRE

    Hritcu, Mihaela

    2013-01-01

    Hritcu, Mihaela. ”Hyväksyvässä ilmapiirissä kannattaisi kaikkien ainakin vierailla”. Ryhmätoiminnan merkitys mielenterveyskuntoutujien elämässä. Helsinki, kevät 2013, 60s., 1 liite. Diakonia-ammattikorkeakoulu, Sosiaalialan koulutusohjelma, sosionomi (AMK). Opinnäytetyön tavoitteena oli selvittää, mikä merkitys on Punaisen Ristin ryhmätoiminnalla mielenterveyskuntoutujan elämässä. Tarkoituksenani on myös tutkimuksen pohjalta tuoda esiin ryhmäläisten näkökulmia ja toiveita ryhmätoiminna...

  2. Varaosasovelluksen suunnittelu ja toteutus ASP.NET-ympäristössä

    OpenAIRE

    Rauhala, Henri

    2015-01-01

    ssä opinnäytetyössä kerrotaan varaosasovelluksen suunnittelusta ja toteutuksesta ASP.NET-ympäristössä Saides Engineering Oy:n asiakkaalle. Kyseisellä asiakkaalla oli ennen projektin aloitusta aiempi versio web-pohjaisesta varaosasovelluksesta, jota haluttiin nyt kehittää kauppapaikan suuntaan. Opinnäytetyössä käydään läpi uuden sovelluksen suunnittelua, ominaisuuksia sekä sovelluksen ohjelmointiratkaisuja. Projektin kehitystiimi koostui itsestäni sekä Saides Engineering Oy:n toimitusjoh...

  3. Tiimityötä kehittämässä Ruskatalojen palveluyhdistyksessä

    OpenAIRE

    Haiko, Anne; Huhtaviita, Eija

    2015-01-01

    TIIVISTELMÄ Haiko, Anne & Huhtaviita, Eija. Tiimityötä kehittämässä Ruskatalojen palveluyhdistyksessä. Diak, Pori, syksy 2015, 65 s., 3liitettä. Diakonia-ammattikorkeakoulu, Sosiaalialan koulutusohjelma, Lasten-, nuorten- ja perheiden hyvinvointi, Sosionomi YAMK. Tässä opinnäytetyössä kehittämisen kohteena oli tiimitoiminta Ruskatalojen palveluyhdistys ry:ssä. Kehittämistyömme tavoitteena oli kehittää tiimityötä yhdistyksessä. Kehittämistyön välineenä käytimme tulevaisuus...

  4. Projektioppiminen yritysympäristössä : Case Dream Hostel & Hotel

    OpenAIRE

    Viira, Juuso

    2015-01-01

    ssä opinnäytetyössä tuotetaan tietoa projektin toteuttamisesta käytännössä hotelli- ja ravintola-alan ammattikorkeakouluopiskelijoiden ja yrityksen välillä. Projektityöskentely on keskeinen opiskelumuoto Tampereen ammattikorkeakoulun hotelli- ja ravintola-alan koulutusohjelmassa. Projektiyhteistyötä toteutetaan yhteistyössä yritysten ja yhdistysten kanssa. Tämän työn tapausesimerkkinä tarkastellaan TAMKin hotelli- ja ravintola-alan opiskelijoiden marraskuussa 2014 toteuttamaa projektia...

  5. Työssäoppimisenohjauksen manuaali : kehitystyö Hotelli Hiltonissa

    OpenAIRE

    Kiviö, Johanna

    2015-01-01

    Tämän opinnäytetyön tavoitteena oli kehittää työssäoppimisen ohjaajille opas opiskelijoiden ohjaamiseen. Opas toteutettiin infografin muodossa. Tutkimuksessa tarkasteltiin työssäoppimisen teoreettisia perusteita, valotettiin millainen on työpaikka oppimisympäristönä ja avattiin työssäoppimisen ohjauksen käsitteitä̈. Lisäksi käsiteltiin hiljaisen tiedon olemusta kokin ammatissa. Kokoavana teoriana esiteltiin Myrkän ja Hätösen ja Friskin ideaalimalli työssäoppimisen ohjaukseen. Kehitys...

  6. Analytical Investigation and Comparison on Performance of Ss316, Ss440c and Titanium Alloy Tool Materials Used As Single Point Cutting Tool

    Directory of Open Access Journals (Sweden)

    Mr. Amaresh Kumar Dhadange

    2015-08-01

    Full Text Available Theoretical analysis for performance studies of SS316, SS44OC and Titanium Alloy used as a cutting tool is presented in this paper. Tool temperature, tools wear and life of the tool is investigated analytically. These theoretical values are compared with the experimental studies conducted by the author. The values obtained from experimental studies are comparable with analytical values and variation is the correlation between theoretical and experimental values is of the order of 15%.

  7. Multimodal pyrethroid resistance in malaria vectors, Anopheles gambiae s.s., Anopheles arabiensis, and Anopheles funestus s.s. in western Kenya.

    Science.gov (United States)

    Kawada, Hitoshi; Dida, Gabriel O; Ohashi, Kazunori; Komagata, Osamu; Kasai, Shinji; Tomita, Takashi; Sonye, George; Maekawa, Yoshihide; Mwatele, Cassian; Njenga, Sammy M; Mwandawiro, Charles; Minakawa, Noboru; Takagi, Masahiro

    2011-01-01

    Anopheles gambiae s.s., Anopheles arabiensis, and Anopheles funestus s.s. are the most important species for malaria transmission. Pyrethroid resistance of these vector mosquitoes is one of the main obstacles against effective vector control. The objective of the present study was to monitor the pyrethroid susceptibility in the 3 major malaria vectors in a highly malaria endemic area in western Kenya and to elucidate the mechanisms of pyrethroid resistance in these species. Gembe East and West, Mbita Division, and 4 main western islands in the Suba district of the Nyanza province in western Kenya were used as the study area. Larval and adult collection and bioassay were conducted, as well as the detection of point mutation in the voltage-gated sodium channel (1014L) by using direct DNA sequencing. A high level of pyrethroid resistance caused by the high frequency of point mutations (L1014S) was detected in An. gambiae s.s. In contrast, P450-related pyrethroid resistance seemed to be widespread in both An. arabiensis and An. funestus s.s. Not a single L1014S mutation was detected in these 2 species. A lack of cross-resistance between DDT and permethrin was also found in An. arabiensis and An. funestus s.s., while An. gambiae s.s. was resistant to both insecticides. It is noteworthy that the above species in the same area are found to be resistant to pyrethroids by their unique resistance mechanisms. Furthermore, it is interesting that 2 different resistance mechanisms have developed in the 2 sibling species in the same area individually. The cross resistance between permethrin and DDT in An. gambiae s.s. may be attributed to the high frequency of kdr mutation, which might be selected by the frequent exposure to ITNs. Similarly, the metabolic pyrethroid resistance in An. arabiensis and An. funestus s.s. is thought to develop without strong selection by DDT. PMID:21853038

  8. Jets of SS433 on scales of dozens parsecs

    CERN Document Server

    Panferov, Alexander A

    2016-01-01

    The radio nebula W50 harbours the relativistic binary system SS433, which is a source of the powerful wind and jets. The origin of W50 is wrapped in the interplay of the wind, supernova remnant and jets. The evolution of the jets on the scales of the nebula is a Rosetta stone for its origin. To disentangle the roles of these components, we study physical conditions of the jets propagation inside W50, and determine deceleration of the jets. The morphology and parameters of the interior of W50 are analyzed using the available observations of the eastern X-ray lobe, which traces the jet. In order to estimate deceleration of this jet, we devised a simplistic model of the viscous interaction, via turbulence, of a jet with the ambient medium, which would fit mass entrainment from the ambient medium into the jets of the radio galaxy 3C31, the well studied case of continuously decelerating jets. X-ray observations suggest that the eastern jet persists through W50 as hollow one, and is recollimated to the opening $\\si...

  9. Evolution of microstructure in laser welding of SS304L

    International Nuclear Information System (INIS)

    Laser welding is an important joining process and its application in industries is growing rapidly. One can produce laser welds over a wide range of process parameters and this offers very good opportunity for producing microstructure of different morphology and scales in the weldment. Weld beads have been produced on 5 mm thick plates of SS304L using CW Nd-YAG laser. Laser power was varied in 200 W to 1000 W range and welding speed was varied in 100 mm/mm to 1000 mm/mm. This resulted in weld beads of different morphology. Microstructure of the weld beads was examined on the cross-section as well as in the axial direction using optical microscopy and Transmission Electron Microscopy (TEM) to study evolution of the microstructure in the weldment. Microstructure was cellular and cellular-dendritic with grains growing from the fusion line towards the centerline. In the central region, cellular growth along the welding direction was observed. The cell size was found to increase with increasing laser power and decreasing welding speed. The findings are presented in this paper. (author)

  10. More on the circumbinary disk of SS 433

    CERN Document Server

    Bowler, M G

    2010-01-01

    Certain lines in spectra of the Galactic microquasar SS 433, in particular the brilliant H alpha line, have been interpreted as emission from a circumbinary disk. In this interpretation the orbital speed of the glowing material is in excess of 200 km/s and the mass of the binary system in excess of 40 solar masses. A very simple model of excitation of disk material is in remarkable agreement with the observations, yet it seems that the very existence of a circumbinary disk is regarded as controversial. Published spectra, taken almost nightly over two orbital periods of the binary system, show H alpha and He I lines; these were analysed as superpositions of Gaussian components. A model in which the excitation of any given patch of putative circumbinary material is proportional to the inverse square of its instantaneous distance from the compact object was constructed and compared with observations. The new model provides an excellent description of the observations. The variation of the H alpha and He I spectr...

  11. Interpretation of observations of the circumbinary disk of SS 433

    CERN Document Server

    Bowler, M G

    2010-01-01

    Context. The Galactic microquasar SS 433 is possessed of a circumbinary disk most clearly seen in the brilliant Balmer H alpha emission line. The orbital speed of the glowing material is an important determinant of the mass of the binary system. The circumbinary disk may be fed through the L2 point and in turn may feed a very extended radio feature known as the ruff. Aims. To present an analysis of spectroscopic optical data from H alpha and He I spectral lines which reveal the circumbinary disk. To use comparisons of the rather different signals to better understand the disk and improve estimates of the rotational speed of the inner rim. To present a simple model which naturally explains some apparently bizarre spectral variations with orbital phase. Methods. Published spectra, taken almost nightly over two orbital periods of the binary system, are analysed. H alpha and He I lines are analysed as superpositions of Gaussian components and a simple model constructed. Results. The data are understood in terms o...

  12. Tritium release from SS316 under vacuum condition

    International Nuclear Information System (INIS)

    The plasma facing surface of the ITER vacuum vessel, partly made of low carbon austenitic stainless steel type 316L, will incorporate tritium during machine operation. In this paper the kinetics of tritium release from stainless steel type 316 into vacuum and into a noble gas stream are compared and modelled. Type 316 stainless steel specimens loaded with tritium either by exposure to 1.2 kPa HT at 573 K or submersion into liquid HTO at 298 K showed characteristic thin surface layers trapping tritium in concentrations far higher than those determined in the bulk. The evolution of the tritium depth profile in the bulk during heating under vacuum was non-discernible from that of tritium liberated into a stream of argon. Only the relative amount of the two released tritium-species, i.e. HT or HTO, was different. Temperature-dependent depth profiles could be predicted with a one-dimensional diffusion model. Diffusion coefficients derived from fitting of the tritium release into an evacuated vessel or a stream of argon were found to be (1.4 ± 1.0)*10-7 and (1.3 ± 0.9)*10-9 cm2/s at 573 and 423 K, respectively. Polished surfaces on type SS316 stainless steel inhibit considerably the thermal release rate of tritium

  13. Tritium release from SS316 under vacuum condition

    Energy Technology Data Exchange (ETDEWEB)

    Torikai, Y.; Penzhorn, R.D. [Hydrogen Isotope Research Center, University of Toyama, Toyama (Japan)

    2015-03-15

    The plasma facing surface of the ITER vacuum vessel, partly made of low carbon austenitic stainless steel type 316L, will incorporate tritium during machine operation. In this paper the kinetics of tritium release from stainless steel type 316 into vacuum and into a noble gas stream are compared and modelled. Type 316 stainless steel specimens loaded with tritium either by exposure to 1.2 kPa HT at 573 K or submersion into liquid HTO at 298 K showed characteristic thin surface layers trapping tritium in concentrations far higher than those determined in the bulk. The evolution of the tritium depth profile in the bulk during heating under vacuum was non-discernible from that of tritium liberated into a stream of argon. Only the relative amount of the two released tritium-species, i.e. HT or HTO, was different. Temperature-dependent depth profiles could be predicted with a one-dimensional diffusion model. Diffusion coefficients derived from fitting of the tritium release into an evacuated vessel or a stream of argon were found to be (1.4 ± 1.0)*10{sup -7} and (1.3 ± 0.9)*10{sup -9} cm{sup 2}/s at 573 and 423 K, respectively. Polished surfaces on type SS316 stainless steel inhibit considerably the thermal release rate of tritium.

  14. Corrosion erosion test of SS316 in flowing Pb Bi

    Science.gov (United States)

    Kikuchi, K.; Kurata, Y.; Saito, S.; Futakawa, M.; Sasa, T.; Oigawa, H.; Wakai, E.; Miura, K.

    2003-05-01

    Corrosion tests of austenitic stainless tube were done under flowing Pb-Bi conditions for 3000 h at 450 °C. Specimens were 316SS produced as a tubing form with 13.8 mm outer diameter, 2 mm thickness and 40 cm length. During operation, maximum temperature, temperature difference and flow velocity of Pb-Bi at the specimen were kept at 450, 50 °C, and 1 m/s, respectively. After the test, specimen and components of the loop were cut and examined by optical microscope, SEM, EDX, WDX and X-ray diffraction. Pb-Bi adhered on the surface of the specimen even after Pb-Bi was drained out to the storage tank from the circulating loop. Results differed from a stagnant corrosion test in that the specimen surface became rough and the corrosion rate was maximally 0.1 mm/3000 h. Mass transfer from the high temperature to the lower temperature area was observed: crystals of Fe-Cr were found on the tube surface in the low-temperature region. The sizes of crystals varied from 0.1 to 0.2 mm. The depositing crystals were ferrite grains and the chemical composition ratio (mass%) of Fe to Cr was 9:1.

  15. Laser Welding of Ultra-Fine Grained Steel SS400

    Institute of Scientific and Technical Information of China (English)

    PENG Yun; TIAN Zhi-ling; CHEN Wu-zhu; WANG Cheng; BAO Gang

    2003-01-01

    The effects of laser welding on microstructure and mechanical properties of ultra-fine grained steel SS400 were studied. The plasma arc welding and MAG welding were conducted to make a comparison between these weldings and laser welding. The coarse grain heat-affected zone (HAZ) of laser welding was simulated using thermomechanical simulation machine, and the impact toughness was tested. The deep penetration laser welding produces weld of large depth and narrow width. The weld metal and HAZ of laser welding was heated and then cooled rapidly. The prior austenite grain size of coarse grain HAZ is 1/10 of that for arc welding. For laser welding, the toughness of weld metal is higher than that of base metal, and the toughness of the coarse grain HAZ of laser welding is on a level with that of base metal. Matching lower laser power with lower welding speed, the hardening tendency of the weld metal and the coarse grain HAZ can be decreased. There is no softened zone. The tensile strength of welded joint formed by laser is higher than that of base metal. The joint has good bending ductility.

  16. 32 CFR Appendix E to Part 246 - Stars and Stripes (S&S) Board of Directors

    Science.gov (United States)

    2010-07-01

    ... from the American Forces Information Service (AFIS) and one S&S may attend the meetings of the other S... DoD policy, the Unified Commands shall ask the Director of the AFIS for resolution. The S&S commander... Unified Commands shall forward the on-going strategic and corporate plan to the Director of the AFIS...

  17. Some inequalities of Ostrowski and Grüss type for triple integrals on time scales

    Directory of Open Access Journals (Sweden)

    Nazir Ahmad Mir

    2011-12-01

    Full Text Available In this paper, we establish some inequalities of Ostrowski and Grüss type for triple integrals on arbitrary time scales involving three functions and their partial derivatives. We also discuss the discrete Ostrowski and Grüss type inequalities for triple sumon time scale.

  18. Some inequalities of Ostrowski and Grüss type for triple integrals on time scales

    OpenAIRE

    Nazir Ahmad Mir; Roman Ullah

    2011-01-01

    In this paper, we establish some inequalities of Ostrowski and Grüss type for triple integrals on arbitrary time scales involving three functions and their partial derivatives. We also discuss the discrete Ostrowski and Grüss type inequalities for triple sumon time scale.

  19. Metabolic and transcriptomic changes induced in Arabidopsis by the rhizobacterium Pseudomonas fluorescens SS101

    NARCIS (Netherlands)

    Mortel, van de J.E.; Vos, de R.C.H.; Dekkers, E.; Pineda, A.; Guillod, L.; Bouwmeester, K.; Loon, van J.J.A.; Dicke, M.; Raaijmakers, J.M.

    2012-01-01

    Systemic resistance induced in plants by nonpathogenic rhizobacteria is typically effective against multiple pathogens. Here, we show that root-colonizing Pseudomonas fluorescens strain SS101 (Pf.SS101) enhanced resistance in Arabidopsis (Arabidopsis thaliana) against several bacterial pathogens, in

  20. Small specimen test techniques for characterizing the mechanical property changes of irradiated AISI SS 316

    International Nuclear Information System (INIS)

    The details of the small punch and ball-indentation test techniques and their standardization for SS 316 which are under progress will also be presented. A comparison of the three test techniques for evaluating the strength and ductility changes of irradiated SS316 are discussed

  1. IRC:n käyttö yritysten viestinnässä

    OpenAIRE

    Klami, Maarit

    2012-01-01

    ssä työssä perehdyttiin IRC:n käyttömahdollisuuksiin yritysten sisäisessä ja ulkoisessa viestinnässä. Työssä verrattiin IRC:tä muihin pikaviestimiin sekä sosiaalisen median tarjoamiin viestintä- ja markkinointimahdollisuuksiin. Eräs tarkastelluista seikoista on yrityksen koon vaikutus siihen, kuinka mittava operaatio IRC:n käyttöönotto yrityksessä on. Työssä on esitetty myös mahdollisuuksia muiden pikaviestimien integroimiseksi IRC:hen. Työ on tehty mielenkiinnosta käyttää IRC:tä viestinnän...

  2. AtlasT4SS: A curated database for type IV secretion systems

    Directory of Open Access Journals (Sweden)

    Souza Rangel C

    2012-08-01

    Full Text Available Abstract Background The type IV secretion system (T4SS can be classified as a large family of macromolecule transporter systems, divided into three recognized sub-families, according to the well-known functions. The major sub-family is the conjugation system, which allows transfer of genetic material, such as a nucleoprotein, via cell contact among bacteria. Also, the conjugation system can transfer genetic material from bacteria to eukaryotic cells; such is the case with the T-DNA transfer of Agrobacterium tumefaciens to host plant cells. The system of effector protein transport constitutes the second sub-family, and the third one corresponds to the DNA uptake/release system. Genome analyses have revealed numerous T4SS in Bacteria and Archaea. The purpose of this work was to organize, classify, and integrate the T4SS data into a single database, called AtlasT4SS - the first public database devoted exclusively to this prokaryotic secretion system. Description The AtlasT4SS is a manual curated database that describes a large number of proteins related to the type IV secretion system reported so far in Gram-negative and Gram-positive bacteria, as well as in Archaea. The database was created using the RDBMS MySQL and the Catalyst Framework based in the Perl programming language and using the Model-View-Controller (MVC design pattern for Web. The current version holds a comprehensive collection of 1,617 T4SS proteins from 58 Bacteria (49 Gram-negative and 9 Gram-Positive, one Archaea and 11 plasmids. By applying the bi-directional best hit (BBH relationship in pairwise genome comparison, it was possible to obtain a core set of 134 clusters of orthologous genes encoding T4SS proteins. Conclusions In our database we present one way of classifying orthologous groups of T4SSs in a hierarchical classification scheme with three levels. The first level comprises four classes that are based on the organization of genetic determinants, shared homologies, and

  3. A novel protein elicitor (SsCut) from Sclerotinia sclerotiorum induces multiple defense responses in plants.

    Science.gov (United States)

    Zhang, Huajian; Wu, Qun; Cao, Shun; Zhao, Tongyao; Chen, Ling; Zhuang, Peitong; Zhou, Xiuhong; Gao, Zhimou

    2014-11-01

    In this study, we report the cloning of the SsCut gene encoding cutinase from Sclerotinia sclerotiorum. We isolated a 609-bp cDNA encoding a polypeptide of 202 amino acids with a molecular weight of 20.4 kDa. Heterologous expression of SsCut in Escherichia coli (His-SsCut) caused the formation of lesions in tobacco that closely resembled hypersensitive response lesions. Mutational analysis identified the C-terminal-half peptide and the same amino acids indispensable for both enzyme and elicitor activity. His-SsCut was caused cell death in Arabidopsis, soybean (Glycine max), oilseed rape (Brassica napus), rice (Oryza sativa), maize (Zea mays), and wheat (Triticum aestivum), indicating that both dicot and monocot species are responsive to the elicitor. Furthermore, the elicitation of tobacco was effective in the induction of the activities of hydrogen peroxide, phenylalanine ammonia-lyase, peroxides, and polyphenol oxidase. His-SsCut-treated plants exhibited enhanced resistance as indicated by a significant reduction in the number and size of S. sclerotiorum, Phytophthora sojae, and P. nicotianae lesions on leaves relative to controls. Real-time PCR results indicated that the expression of defense-related genes and genes involved in signal transduction were induced by His-SsCut. Our results demonstrate that SsCut is an elicitor that triggers defense responses in plants and will help to clarify its relationship to downstream signaling pathways that induce defense responses. PMID:25149470

  4. TIMES-SS - A mechanistic evaluation of an external validation study using reaction chemistry principles

    DEFF Research Database (Denmark)

    Roberts, David W.; Patlewicz, Grace; Dimitrov, Sabcho D.;

    2007-01-01

    The TImes MEtabolism Simulator platform used for predicting skin sensitization (TIMES-SS) is a hybrid expert system that was developed at Bourgas University using funding and data from a consortium comprised of industry and regulators. TIMES-SS encodes structure-toxicity and structure-skin metabo......The TImes MEtabolism Simulator platform used for predicting skin sensitization (TIMES-SS) is a hybrid expert system that was developed at Bourgas University using funding and data from a consortium comprised of industry and regulators. TIMES-SS encodes structure-toxicity and structure...... chemicals in the murine local lymph node assay (LLNA) and then compared with predictions made by TIMES-SS. The results were promising with an overall good concordance (83%) between experimental and predicted values. The LLNA results were evaluated with respect to reaction chemistry principles for...... sensitization. Additional testing on a further four chemicals was carried out to explore some of the specific reaction chemistry findings in more detail. Improvements for TIMES-SS, where appropriate, were put forward together with proposals for further research work. TIMES-SS is a promising tool to aid in the...

  5. Responsiivinen web-kehitys WordPress-julkaisujärjestelmässä

    OpenAIRE

    Jutila, Tiina

    2013-01-01

    Opinnäytetyössä tutkittiin responsiivisen web-kehityksen toteuttamista WordPress-julkaisujärjes¬-telmässä. Tavoitteena oli toteuttaa yrityksen käyttöön WordPress-teema, joka sisälsi tarvittavat ominaisuudet mukautuvien verkkosivujen toteuttamiseen. Teeman tarkoituksena oli toimia pohjana, jonka päälle verkkosivun rakentaminen olisi mahdollisimman vaivatonta. Työssä käsiteltiin mobiililaitteiden asettamia haasteita web-kehityksessä, sekä tekniikoita niiden rat-kaisemiseen. Päätelaitteiden ...

  6. Valokuvaus 4-vuotiaiden päiväkotiryhmässä

    OpenAIRE

    Alastalo, Kata

    2014-01-01

    Toiminnallisessa opinnäytetyössä tutkittiin voiko valokuvauksen tuoda yhdeksi työmenetelmäksi Porin kaupungin Kuvataidepäiväkoti Viikarin 4-vuotiaiden Naperot-ryhmään. Toiminnallisiin osuuksiin osallistui yhteensä 17 lasta, jotka kaikki olivat syntyneet vuonna 2009. Opinnäytetyössä pyrittiin lapsilähtöisyyden lisäämiseen ja positiivisen minäkuvan tukemiseen valokuvauksen keinoin. Opinnäytetyön teoriaosuudessa käsiteltiin taidekasvatusta ja sen osuutta hyvinvointiin. Opinnäytetyössä perehd...

  7. Direct Evidence that RNA Inhibits APOBEC3G ssDNA Cytidine Deaminase Activity

    OpenAIRE

    McDougall, William M.; Smith, Harold C.

    2011-01-01

    APOBEC3G (A3G) is a deoxycytidine deaminase active on ssDNA substrates. In HIV infected cells A3G interacted with reverse transcription complexes where its activity as a deoxycytidine deaminase led to mutation of the viral genome. A3G not only bound ssDNA, but it also had an intrinsic ability to bind RNA. In many cell types that can support HIV replication, A3G ssDNA deaminase activity was suppressed and the enzyme resided in high molecular mass, ribonucleoprotein complexes associated with cy...

  8. Vaatemerkin SS14-malliston kehittäminen, Case: White Trash Gospel

    OpenAIRE

    Itäkylä, Juho

    2014-01-01

    Tämän opinnäytetyön aiheena on toimeksiantajayrityksen SS -14 vaatemalliston kehittäminen. Toimeksiantajana on helsinkiläinen, toimintaansa aloittava vaatemerkki, White Trash Gospel ja hankkeen taustalla on White Trash Gospelin tarve kehittää mallistoaan kilpailijoiden toimia ja asiakkaiden mielipiteitä silmällä pitäen. Opinnäytetyön tavoitteena on White Trash Gospel –vaatemerkin SS14-malliston kehittäminen potentiaalisille asiakkaille. Työssä kiinnitetään huomiota markkinointia ajatellen...

  9. Kouluruokailusuositusten toteutuminen Lempäälässä : Kouluruoan ravitsemuslaatu

    OpenAIRE

    Rönö, Kaisu

    2010-01-01

    Valtion ravitsemusneuvottelukunnan antama Kouluruokailusuositus (2008) toimi tämän opinnäytetyön innoittajana. Opinnäytetyön empiirinen osa toteutettiin yhteistyössä Lempäälän ruokapalvelun kanssa. Tässä työssä selvitettiin, onko Lempäälän kouluruoka ravitsemussuositusten mukaista. Empiirisessä osassa tehtiin Sydänliiton ruoan ravitsemuslaatua mittaava kysely Lempäälän kolmelle kouluruokaa valmistavalle keittiölle. Kyselyn perusteella selvitettiin, toteutuiko keittiöillä Sydänliiton perus-, r...

  10. Liikkeenharjoittaja vuokralaisena asunto-osakeyhtiössä sijaitsevassa liiketilassa - osapuolten välinen vastuunjako

    OpenAIRE

    Santasalo, Hanna

    2014-01-01

    Diplomityössä tutkittiin sitä, millainen on lainsäädännön mukainen vastuunjako osapuolten välillä silloin, kun liiketoiminnanharjoittaja on vuokralaisena asunto-osakeyhtiössä sijaitsevassa liiketilassa. Lisäksi selvitettiin tyypillisimpiä ongelma- ja riitatilanteita eri osapuolten välillä. Teoreettisen tutkimuksen lisäksi tavoitteena oli luoda Suomen Lähikauppa Oy:lle asunto-osakeyhtiön erityispiirteet huomioiva vuokrasopimusmalli sekä asunto-osakeyhtiössä sijaitsevan liiketilan vuokraukseen ...

  11. Synthesis and electrochemical characterization of porous niobium oxide coated 316L SS for orthopedic applications

    International Nuclear Information System (INIS)

    Niobium oxide was prepared using sol-gel process and coated on 316L stainless steel (SS) substrate via dip-coating technique. The surface characterization results after a thermal treatment revealed that the coated surface was porous, uniform and well crystalline on the substrate. The corrosion resistance and bioactivity of the porous niobium oxide coated 316L SS in simulated body fluid (SBF) solution was evaluated. The in vitro test revealed a layer of carbonate-containing apatite formation over the coated porous surface. The results indicated that the porous niobium oxide coated 316L SS exhibited a high corrosion resistance and an enhanced biocompatibility in SBF solution.

  12. Ennustaminen perinteisen budjetoinnin tukena : Case: Osuuskauppa PeeÄssä

    OpenAIRE

    Nevalainen, Senja

    2014-01-01

    Opinnäytetyön aiheena on ennustamisen käyttäminen perinteistä budjetointia tukevana toimintona. Työn toimeksiantajana on Osuuskauppa PeeÄssä, joka on Pohjois-Savossa toimiva palveluyritys. Opinnäytetyön tavoitteena oli selvittää Osuuskauppa PeeÄssässä ennustamista tekevien henkilöiden mielipiteitä ja ajatuksia ennustamisesta kahden kyselyn avulla. Ensimmäisen kyselyn tarkoituksena oli selvittää, millaisia ennakko-odotuksia käyttäjillä on ennustamisesta ennen ennustamisen aloittamista Suprossa...

  13. Lastentarhanopettajien kokemuksia tunteiden siirtymisestä päiväkotityössä

    OpenAIRE

    Engström, Leena

    2012-01-01

    Engström, Leena. Lastentarhanopettajien kokemuksia tunteiden siirtymisestä päiväkotityössä. Diak Etelä, Helsinki, kevät 2012, 51 s., 4 liitettä. Diakonia-ammattikorkeakoulu, Sosiaalialan koulutusohjelma, Sosiaali- ja kasvatusalan suuntautumisvaihtoehto, sosionomi (AMK) + lastentarhanopettajan virkakelpoisuus. Opinnäytetyössä tutkittiin lastentarhanopettajien kokemuksia omien tunteiden siirtymisestä päiväkotityössä. Teoriaosuus käsitteli päiväkodin varhaiskasvatus-ta ja lastentarh...

  14. Ääniä päässä : Äänisuunnitelman tekeminen teoriassa ja käytännössä

    OpenAIRE

    Hulkko, Teemu

    2015-01-01

    Tutkin opinnäytetyössäni äänisuunnittelijan roolia tuotannossa sekä äänisuunnitelman toteutusta teoriassa ja käytännössä. Tavoitteenani oli kirjoittaa havainnollistava teos äänisuunnittelusta ja sen mahdollisuuksista osana elokuvaa. Työ olen toteuttanut tutustumalla äänisuunnittelusta kertovaan kirjallisuuteen ja poiminut kohtauksia esimerkeiksi kuudesta eri elokuvasta. Kootun aineiston pohjalta kirjoitetun opinnäytetyön teoriaosan perusteella olen tehnyt äänisuunnitelman Keijujen koti -l...

  15. Facebookin hyödyntäminen koripalloseurojen viestinnässä

    OpenAIRE

    Siven, Kim

    2012-01-01

    ssä opinnäytetyössä selvitetään Facebookin roolia koripalloseurojen viestinnässä. Facebook ja koripallo ovat molemmat tällä hetkellä ajankohtaisia aiheita. Työssä perehdytään myös sosiaalisen median hyötyihin yritykselle sekä asiakkaalle. Opinnäytetyö tehtiin syksyn 2011 ja kevään 2012 välisenä aikana. Työn tarkoitus on tutkia käyttävätkö seurat Facebookia ja millä tavoin ne ovat yhteydessä kuluttajiin. Lisäksi selvitetään käyttävätkö koripalloseurat koko Facebookin kapasiteettia markki...

  16. Changes of plasma SS, SP contents in adult patients with primary hypothyroidism

    International Nuclear Information System (INIS)

    Objective: To investigate the possible mechanism of imparirment of central nervous system function in hypothyroid patients through determination of changes of plasma neuropeptides after thyroid hormone replacement therapy. Methods: Plasma somatostatin (SS) and substance P(SP) contents were measured with RIA in 45 patients with primary hypothyroidism both before and after thyroid hormone replacement therapy as well as in 38 controls. Results: Before treatment, the plasma contents of SP in the patients were significantly lower than those in the controls (P0.05). However, the plasma contents of SS in the more advanced hypothyroid patients with FT33, FT4 levels, the plasma SS, SP increased significantly (vs before treatment P<0.05, P<0.01). Conclusion: The decrease of plasma contents of SS and SP in patients with hypothyroidism might be related to the development of psycho-neurological symptoms in these patients and thyroid hormone replacement therapy was desirable. (authors)

  17. Ammattiosaamisen näytöt yrityksen ja ammattiopiston yhteistyössä

    OpenAIRE

    Tuomainen, Kari

    2011-01-01

    Opinnäytetyön aiheena oli selvittää, miten ammattiosaamisen näytöt toimivat paikallisen osuuskaupan ja ammattiopiston yhteistyössä koskien ravintolan salipuolen tarjoilijoita. Ammattiosaamisen näytöt ovat olleet käytössä työssäoppijoiden arvioinnissa viisi vuotta. Tavoitteena oli saada tietoa näyttöjen hyödyistä työantajanäkökulmasta katsottuna sekä löytää mahdollisia kehittämiskohteita. Opinnäytetyön teoreettisessa osassa selvennettiin työssäoppimisen ja ammattiosaamisen näytön käsittei...

  18. Klusterin toiminnan tutkiminen Linux-ympäristössä

    OpenAIRE

    Paavola, Jussi

    2010-01-01

    Työssä on tarkoitus tutkia, että minkälaisia klusterointimahdollisuuksia on tällä hetkellä tarjolla Linux-ympäristöön. Työssä käydään lyhyesti läpi Linux käyttöjärjestelmää ja klusterointia yleisesti. Tarkemmin työssä tutustutaan erilaisiin vaihtoehtoihin, mitä Linuxille on tarjolla klusterointiin. Klusteroinnin yhteydessä, tutkitaan myös hieman pilvitekniikkaa. Työssä rakennetaan myös oma klusteri koulun tietoliikenne-laboratoriossa. The purpose of this thesis was to explore what kin...

  19. Naisten ja miesten eriarvoisuus työelämässä Suomessa

    OpenAIRE

    Aho, Emilia

    2014-01-01

    Työn tavoitteena on tutkia syitä naisten ja miesten eriarvoiseen asemaan työelämässä. Työssä keskitytään etenkin palkkaeroihin, joista naisten ja miesten eriarvoisuus työelämässä on nähtävissä selvästi. Jotta tasa-arvoisuuteen päästäisiin naisten ja miesten välillä, on pyrittävä tukemaan heikommassa asemassa olevia. Työnantajan tulee aktiivisesti edistää tasa-arvoa työelämässä, ja tasa-arvosuunnitelman laatiminen on yksi askel kohti tasa-arvoisempaa työyhteisöä. Lainsäädäntö asettaa vaat...

  20. TIMES-SS - A mechanistic evaluation of an external validation study using reaction chemistry principles

    DEFF Research Database (Denmark)

    Roberts, David W.; Patlewicz, Grace; Dimitrov, Sabcho D.; Low, Lawrence K.; Aptula, Aynur O.; Kern, Petra S.; Dimitrova, Gergana D.; Comber, Mike I.H.; Phillips, Richard D.; Niemela, Jay; Madsen, Charlotte Bernhard; Wedebye, Eva Bay; Bailey, Paul T.; Mekenyan, Ovanes G.

    2007-01-01

    The TImes MEtabolism Simulator platform used for predicting skin sensitization (TIMES-SS) is a hybrid expert system that was developed at Bourgas University using funding and data from a consortium comprised of industry and regulators. TIMES-SS encodes structure-toxicity and structure-skin metabo......The TImes MEtabolism Simulator platform used for predicting skin sensitization (TIMES-SS) is a hybrid expert system that was developed at Bourgas University using funding and data from a consortium comprised of industry and regulators. TIMES-SS encodes structure-toxicity and structure......-skin metabolism relationships through a number of transformations, some of which are underpinned by mechanistic three-dimensional quantitative structure-activity relationships. Here, we describe an external validation exercise that was recently carried out. As part of this exercise, data were generated for 40 new...

  1. As-rigid-as-possible mosaicking and serial section registration of large ssTEM datasets

    OpenAIRE

    Saalfeld, Stephan; Cardona, Albert; Hartenstein, Volker; Tomančák, Pavel

    2010-01-01

    Motivation: Tiled serial section Transmission Electron Microscopy (ssTEM) is increasingly used to describe high-resolution anatomy of large biological specimens. In particular in neurobiology, TEM is indispensable for analysis of synaptic connectivity in the brain. Registration of ssTEM image mosaics has to recover the 3D continuity and geometrical properties of the specimen in presence of various distortions that are applied to the tissue during sectioning, staining and imaging. These includ...

  2. SS18-SSX2 and the mitochondrial apoptosis pathway in mouse and human synovial sarcomas

    OpenAIRE

    Jones, Kevin B.; Su, Le; Jin, Huifeng; Lenz, Carol; Randall, R. Lor; Underhill, T. Michael; Nielsen, Torsten O.; Sharma, Sunil; Capecchi, Mario R.

    2012-01-01

    Synovial sarcoma is a deadly malignancy with limited sensitivity to traditional cytotoxic chemotherapy. SS18-SSX fusion oncogene expression characterizes human synovial sarcomas and drives oncogenesis in a mouse model. Elevated expression of BCL2 is considered a consistent feature of the synovial sarcoma expression profile. Our objective was to evaluate the expression of apoptotic pathway members in synovial sarcomas and interrogate the impact of modulating SS18-SSX expression on this pathway...

  3. Integrated Composite Stiffener Structure (ICoSS) Concept for Planetary Entry Vehicles

    Science.gov (United States)

    Kellas, Sotiris

    2016-01-01

    Results from the design, manufacturing, and testing of a lightweight Integrated Composite Stiffened Structure (ICoSS) concept, intended for multi-mission planetary entry vehicles are presented. Tests from both component and full-scale tests for a typical Earth Entry Vehicle forward shell manufactured using the ICoSS concept are presented and advantages of the concept for the particular application of passive Earth Entry Vehicles over other structural concepts are discussed.

  4. Viittomakielinen kirkkokäsikirja tulkkien työssä

    OpenAIRE

    Piutunen, Anna; Kaunisto, Kirsi

    2011-01-01

    TIIVISTELMÄ Kaunisto, Kirsi & Piutunen, Anna. Viittomakielinen kirkkokäsikirja tulkkien työssä. Diak Länsi, Turku, kevät 2011, 57 s. 2 liitettä. Diakonia-ammattikorkeakoulu, Viittomakielentulkin koulutusohjelma, viittomakielen tulkki (AMK). Tutkimuksen tavoitteena oli kartoittaa viittomakielentulkkien suhtautumista Kirkolliskokouksen hyväksymiin virallisiin viittomakielisiin kirkollisiin käännöksiin. Työssä tutkittiin, käyttävätkö tulkit hengellisissä tilaisuuksissa tulkatessaan vi...

  5. Bessel and Grüss Type Inequalities in Inner Product Modules over Banach -Algebras

    Directory of Open Access Journals (Sweden)

    Dragomir SS

    2011-01-01

    Full Text Available We give an analogue of the Bessel inequality and we state a simple formulation of the Grüss type inequality in inner product -modules, which is a refinement of it. We obtain some further generalization of the Grüss type inequalities in inner product modules over proper -algebras and unital Banach -algebras for -seminorms and positive linear functionals.

  6. Toxicity of Neurons Treated with Herbicides and Neuroprotection by Mitochondria-Targeted Antioxidant SS31

    OpenAIRE

    Hemachandra Reddy, P.; Byung Park; Ulziibat Shirendeb; Reddy, Arubala P.; Calkins, Marcus J.; Peizhong Mao; Reddy, Tejaswini P.; Maria Manczak

    2011-01-01

    The purpose of this study was to determine the neurotoxicity of two commonly used herbicides: picloram and triclopyr and the neuroprotective effects of the mitochondria-targeted antioxidant, SS31. Using mouse neuroblastoma (N2a) cells and primary neurons from C57BL/6 mice, we investigated the toxicity of these herbicides, and protective effects of SS1 peptide against picloram and triclopyr toxicity. We measured total RNA content, cell viability and mRNA expression of peroxiredoxins, neuroprot...

  7. CLaSS Computer Literacy Software: From Design to Implementation - A Three Year Student Evaluation

    OpenAIRE

    Ian Cole

    2006-01-01

    Both computer literacy and information retrieval techniques are required to undertake studies in higher education in the United Kingdom. This paper considers the research, development and the 3-year student evaluation of a piece of learning technology in computer and information literacy (CLaSS software). Students completed a questionnaire to examine their own assessment of knowledge and competence in computer and information literacy and based on this assessment CLaSS software was created to...

  8. A SELEX Protocol to Identify ssDNA Biotemplates for Gold Nanoparticle Synthesis

    OpenAIRE

    Calabrese, Philip

    2015-01-01

    A modified ssDNA SELEX protocol was developed in order to evolve a randomized library of imidazole modified ssDNA sequences towards sequences that mediate the formation of gold nanoparticles from Au3+ precursor ions in aqueous solution. Active sequences bound to nanoparticles were partitioned from inactive sequences based on density via ultracentrifugation through a discontinuous sucrose gradient. Colloidal gold solutions produced by the evolved pool had a distinct absorbance spectra and prod...

  9. Characteristic evaluation of HIP bonded SS/DSCu joints for surface roughness

    International Nuclear Information System (INIS)

    Bonding technologies of SS/DSCu, SS/SS and DSCu/DSCu using HIP method have been developed for the fabrication of the ITER shielding blanket first wall. In this study, the effect of the roughness of the HIP bonding surface has been studied for the 1-40 μm roughness from the view points of bondability of surfaces by HIPping and simplification of fabrication procedure. For the tensile properties, it has been found that the effect of the roughness within above range is negligibly small. On the other hand, it has been confirmed that the finer the surface roughness, the higher impact properties for the DSCu/DSCu joints. Significant differences are not found in the SS316L/DSCu and SS316L/SS316L joints with 1-40 μm, though the finer the surface roughness, the slightly higher the impact energy value. Also, it has been found that the HIP bonded joint with good bondability is obtained by using higher HIP pressure, i.e. 200 MPa even with coarser surface roughness, i.e. ∼10 μm, to reduce the machining requirement in the fabrication procedure. In the fabrication of the International Thermo-nuclear Experimental Reactor (ITER) shielding blanket, conditions of the surface roughness and the Hot Isostatic Pressing (HIP) pressure will be selected by taking into account the machining cost for surface preparation and the HIP process cost based on dimensions of the part to be HIP bonded

  10. Novel immunocytokine IL12-SS1 (Fv) inhibits mesothelioma tumor growth in nude mice.

    Science.gov (United States)

    Kim, Heungnam; Gao, Wei; Ho, Mitchell

    2013-01-01

    Mesothelin is a glycosylphosphatidylinositol-anchored glycoprotein that is highly expressed on the cell surface of malignant mesothelioma. Monoclonal antibodies against mesothelin are being evaluated for the treatment of mesothelioma. Immunocytokines represent a novel class of armed antibodies. To provide an alternative approach to current mesothelin-targeted antibody therapies, we have developed a novel immunocytokine based on interleukin-12 (IL12) and the SS1 Fv specific for mesothelin. IL12 possesses potent anti-tumor activity in a wide variety of solid tumors. The newly-developed recombinant immunocytokine, IL12-SS1 (Fv), was produced in insect cells using a baculovirus-insect cell expression system. The SS1 single-chain Fv was fused to the C terminus of the p35 subunit of IL12 through a short linker (GSADGG). The single-chain IL12-SS1 (Fv) immunocytokine bound native mesothelin proteins on malignant mesothelioma (NCI-H226) and ovarian (OVCAR-3) cells as well as recombinant mesothelin on A431/H9 cells. The immunocytokine retained sufficient bioactivity of IL12 and significantly inhibited human malignant mesothelioma (NCI-H226) grown in the peritoneal cavity of nude mice and showed comparable anti-tumor activity to that of the SS1P immunotoxin. IL12-SS1 (Fv) is the first reported immunocytokine to mesothelin-positive tumors and may be an attractive addition to mesothelin-targeted cancer therapies. PMID:24260587

  11. Novel immunocytokine IL12-SS1 (Fv inhibits mesothelioma tumor growth in nude mice.

    Directory of Open Access Journals (Sweden)

    Heungnam Kim

    Full Text Available Mesothelin is a glycosylphosphatidylinositol-anchored glycoprotein that is highly expressed on the cell surface of malignant mesothelioma. Monoclonal antibodies against mesothelin are being evaluated for the treatment of mesothelioma. Immunocytokines represent a novel class of armed antibodies. To provide an alternative approach to current mesothelin-targeted antibody therapies, we have developed a novel immunocytokine based on interleukin-12 (IL12 and the SS1 Fv specific for mesothelin. IL12 possesses potent anti-tumor activity in a wide variety of solid tumors. The newly-developed recombinant immunocytokine, IL12-SS1 (Fv, was produced in insect cells using a baculovirus-insect cell expression system. The SS1 single-chain Fv was fused to the C terminus of the p35 subunit of IL12 through a short linker (GSADGG. The single-chain IL12-SS1 (Fv immunocytokine bound native mesothelin proteins on malignant mesothelioma (NCI-H226 and ovarian (OVCAR-3 cells as well as recombinant mesothelin on A431/H9 cells. The immunocytokine retained sufficient bioactivity of IL12 and significantly inhibited human malignant mesothelioma (NCI-H226 grown in the peritoneal cavity of nude mice and showed comparable anti-tumor activity to that of the SS1P immunotoxin. IL12-SS1 (Fv is the first reported immunocytokine to mesothelin-positive tumors and may be an attractive addition to mesothelin-targeted cancer therapies.

  12. Large short- and long-term Hikurangi margin, New Zealand SSEs in 2013, and possible triggering of moderate to large seismicity during the SSEs

    Science.gov (United States)

    Wallace, L. M.; Fry, B.; Bannister, S. C.; Hamling, I. J.; Ito, Y.; Obara, K.

    2013-12-01

    The Hikurangi margin has been the site of 3 large slow slip events (SSEs) during the 2013 period. These include a recurrence of the deep (30-50 km depth) long-term Kapiti SSE that began in January 2013, as well as a large, shallow (Mw 6.8, Tohoku University to capture vertical seafloor deformation in Hikurangi SSEs. These 3 SSEs further demonstrate the remarkable diversity of slow slip behavior at the Hikurangi margin, particularly in terms of recurrence, depths, duration, and magnitudes. In addition to SSE activity, 2013 has also seen a swarm of earthquakes (up to ML 4.8) during the Hawkes Bay region SSE. On July 21, 2013, the largest earthquake to strike the Wellington region (Mw 6.5) since 1942 occurred on a NE-striking strike-slip fault at about 13 km focal depth, within the overriding plate beneath Cook Strait. The event was preceded by foreshocks reaching Mw 5.7 that had predominantly reverse focal mechanisms and somewhat deeper depths. We present evidence that this earthquake sequence may have been promoted by stress changes from the ongoing Kapiti SSE. We also show evidence for nonvolcanic tremor during the Kapiti SSEs.

  13. An improved DNA force field for ssDNA interactions with gold nanoparticles

    International Nuclear Information System (INIS)

    The widespread applications of single-stranded DNA (ssDNA) conjugated gold nanoparticles (AuNPs) have spurred an increasing interest in the interactions between ssDNA and AuNPs. Despite extensive studies using the most sophisticated experimental techniques, the detailed molecular mechanisms still remain largely unknown. Large scale molecular dynamics (MD) simulations can thus be used to supplement experiments by providing complementary information about ssDNA-AuNP interactions. However, up to now, all modern force fields for DNA were developed based on the properties of double-stranded DNA (dsDNA) molecules, which have hydrophilic outer backbones “protecting” hydrophobic inner nucleobases from water. Without the double-helix structure of dsDNA and thus the “protection” by the outer backbone, the nucleobases of ssDNA are directly exposed to solvent, and their behavior in water is very different from that of dsDNA, especially at the interface with nanoparticles. In this work, we have improved the force field of ssDNA for use with nanoparticles, such as AuNPs, based on recent experimental results and quantum mechanics calculations. With the new improved force field, we demonstrated that a poly(A) sequence adsorbed on a AuNP surface is much more stable than a poly(T) sequence, which is consistent with recent experimental observations. On the contrary, the current standard force fields, including AMBER03, CHARMM27, and OPLSAA, all gave erroneous results as compared to experiments. The current improved force field is expected to have wide applications in the study of ssDNA with nanomaterials including AuNPs, which might help promote the development of ssDNA-based biosensors and other bionano-devices

  14. Toxicity of Neurons Treated with Herbicides and Neuroprotection by Mitochondria-Targeted Antioxidant SS31

    Directory of Open Access Journals (Sweden)

    P. Hemachandra Reddy

    2011-01-01

    Full Text Available The purpose of this study was to determine the neurotoxicity of two commonly used herbicides: picloram and triclopyr and the neuroprotective effects of the mitochondria-targeted antioxidant, SS31. Using mouse neuroblastoma (N2a cells and primary neurons from C57BL/6 mice, we investigated the toxicity of these herbicides, and protective effects of SS1 peptide against picloram and triclopyr toxicity. We measured total RNA content, cell viability and mRNA expression of peroxiredoxins, neuroprotective genes, mitochondrial- encoded electron transport chain (ETC genes in N2a cells treated with herbicides and SS31. Using primary neurons from C57BL/6 mice, neuronal survival was studied in neurons treated with herbicides, in neurons pretreated with SS31 plus treated with herbicides, neurons treated with SS31 alone, and untreated neurons. Significantly decreased total RNA content, and cell viability in N2a cells treated with picloram and triclopyr were found compared to untreated N2a cells. Decreased mRNA expression of neuroprotective genes, and ETC genes in cells treated with herbicides was found compared to untreated cells. Decreased mRNA expression of peroxiredoxins 1–6 in N2a cells treated with picloram was found, suggesting that picloram affects the antioxidant enzymes in N2a cells. Immunofluorescence analysis of primary neurons revealed that decreased neuronal branching and degenerating neurons in neurons treated with picloram and triclopyr. However, neurons pretreated with SS31 prevented degenerative process caused by herbicides. Based on these results, we propose that herbicides—picloram and triclopyr appear to damage neurons, and the SS31 peptide appears to protect neurons from herbicide toxicity.

  15. Toxicity of neurons treated with herbicides and neuroprotection by mitochondria-targeted antioxidant SS31.

    Science.gov (United States)

    Reddy, Tejaswini P; Manczak, Maria; Calkins, Marcus J; Mao, Peizhong; Reddy, Arubala P; Shirendeb, Ulziibat; Park, Byung; Reddy, P Hemachandra

    2011-01-01

    The purpose of this study was to determine the neurotoxicity of two commonly used herbicides: picloram and triclopyr and the neuroprotective effects of the mitochondria-targeted antioxidant, SS31. Using mouse neuroblastoma (N2a) cells and primary neurons from C57BL/6 mice, we investigated the toxicity of these herbicides, and protective effects of SS1 peptide against picloram and triclopyr toxicity. We measured total RNA content, cell viability and mRNA expression of peroxiredoxins, neuroprotective genes, mitochondrial-encoded electron transport chain (ETC) genes in N2a cells treated with herbicides and SS31. Using primary neurons from C57BL/6 mice, neuronal survival was studied in neurons treated with herbicides, in neurons pretreated with SS31 plus treated with herbicides, neurons treated with SS31 alone, and untreated neurons. Significantly decreased total RNA content, and cell viability in N2a cells treated with picloram and triclopyr were found compared to untreated N2a cells. Decreased mRNA expression of neuroprotective genes, and ETC genes in cells treated with herbicides was found compared to untreated cells. Decreased mRNA expression of peroxiredoxins 1-6 in N2a cells treated with picloram was found, suggesting that picloram affects the antioxidant enzymes in N2a cells. Immunofluorescence analysis of primary neurons revealed that decreased neuronal branching and degenerating neurons in neurons treated with picloram and triclopyr. However, neurons pretreated with SS31 prevented degenerative process caused by herbicides. Based on these results, we propose that herbicides--picloram and triclopyr appear to damage neurons, and the SS31 peptide appears to protect neurons from herbicide toxicity. PMID:21318024

  16. An improved DNA force field for ssDNA interactions with gold nanoparticles

    Energy Technology Data Exchange (ETDEWEB)

    Jiang, Xiankai; Huai, Ping; Fan, Chunhai; Song, Bo, E-mail: ruhongz@us.ibm.com, E-mail: bosong@sinap.ac.cn [Shanghai Institute of Applied Physics, Chinese Academy of Sciences, P. O. Box 800-204, Shanghai 201800 (China); Gao, Jun [School of Chemistry and Chemical Engineering, Shandong University, Jinan 250100 (China); Huynh, Tien; Zhou, Ruhong, E-mail: ruhongz@us.ibm.com, E-mail: bosong@sinap.ac.cn [IBM Thomas J. Watson Research Center, Yorktown Heights, New York 10598 (United States)

    2014-06-21

    The widespread applications of single-stranded DNA (ssDNA) conjugated gold nanoparticles (AuNPs) have spurred an increasing interest in the interactions between ssDNA and AuNPs. Despite extensive studies using the most sophisticated experimental techniques, the detailed molecular mechanisms still remain largely unknown. Large scale molecular dynamics (MD) simulations can thus be used to supplement experiments by providing complementary information about ssDNA-AuNP interactions. However, up to now, all modern force fields for DNA were developed based on the properties of double-stranded DNA (dsDNA) molecules, which have hydrophilic outer backbones “protecting” hydrophobic inner nucleobases from water. Without the double-helix structure of dsDNA and thus the “protection” by the outer backbone, the nucleobases of ssDNA are directly exposed to solvent, and their behavior in water is very different from that of dsDNA, especially at the interface with nanoparticles. In this work, we have improved the force field of ssDNA for use with nanoparticles, such as AuNPs, based on recent experimental results and quantum mechanics calculations. With the new improved force field, we demonstrated that a poly(A) sequence adsorbed on a AuNP surface is much more stable than a poly(T) sequence, which is consistent with recent experimental observations. On the contrary, the current standard force fields, including AMBER03, CHARMM27, and OPLSAA, all gave erroneous results as compared to experiments. The current improved force field is expected to have wide applications in the study of ssDNA with nanomaterials including AuNPs, which might help promote the development of ssDNA-based biosensors and other bionano-devices.

  17. Analysis of the stress-inducible transcription factor SsNAC23 in sugarcane plants

    Directory of Open Access Journals (Sweden)

    Renata Fava Ditt

    2011-08-01

    Full Text Available Stresses such as cold and drought can impair plant yield and induce a highly complex array of responses. Sugarcane (Saccharum spp. is cultivated in tropical and subtropical areas and is considered a cold-sensitive plant. We previously showed that cold stress induces the expression of several genes in in vitro sugarcane plantlets. Here we characterize one of those genes, SsNAC23, a member of the NAC family of plant-specific transcription factors, which are induced by low temperature and other stresses in several plant species. The expression of SsNAC23 was induced in sugarcane plants exposed to low temperatures (4ºC. With the aim of further understanding the regulatory network in response to stress, we used the yeast two-hybrid system to identify sugarcane proteins that interact with SsNAC23. Using SsNAC23 as bait, we screened a cDNA expression library of sugarcane plants submitted to 4ºC for 48 h. Several interacting partners were identified, including stress-related proteins, increasing our knowledge on how sugarcane plants respond to cold stress. One of these interacting partners, a thioredoxin h1, offers insights into the regulation of SsNAC23 activity.

  18. Structure/property (constitutive and dynamic strength/damage) characterization of additively manufactured 316L SS

    Science.gov (United States)

    Gray, G. T., III; Livescu, V.; Rigg, P. A.; Trujillo, C. P.; Cady, C. M.; Chen, S. R.; Carpenter, J. S.; Lienert, T. J.; Fensin, S.

    2015-09-01

    For additive manufacturing (AM), the certification and qualification paradigm needs to evolve as there exists no "ASTM-type" additive manufacturing certified process or AM-material produced specifications. Accordingly, utilization of AM materials to meet engineering applications requires quantification of the constitutive properties of these evolving materials in comparison to conventionally-manufactured metals and alloys. Cylinders of 316L SS were produced using a LENS MR-7 laser additive manufacturing system from Optomec (Albuquerque, NM) equipped with a 1kW Yb-fiber laser. The microstructure of the AM-316L SS is detailed in both the as-built condition and following heat-treatments designed to obtain full recrystallization. The constitutive behavior as a function of strain rate and temperature is presented and compared to that of nominal annealed wrought 316L SS plate. The dynamic damage evolution and failure response of all three materials was probed using flyer-plate impact driven spallation experiments at a peak stress of 4.5 GPa to examine incipient spallation response. The spall strength of AM-produced 316L SS was found to be very similar for the peak shock stress studied to that of annealed wrought or AM-316L SS following recrystallization. The damage evolution as a function of microstructure was characterized using optical metallography.

  19. Direct Evidence that RNA Inhibits APOBEC3G ssDNA Cytidine Deaminase Activity

    Science.gov (United States)

    McDougall, William M.; Smith, Harold C.

    2011-01-01

    APOBEC3G (A3G) is a deoxycytidine deaminase active on ssDNA substrates. In HIV infected cells A3G interacted with reverse transcription complexes where its activity as a deoxycytidine deaminase led to mutation of the viral genome. A3G not only bound ssDNA, but it also had an intrinsic ability to bind RNA. In many cell types that can support HIV replication, A3G ssDNA deaminase activity was suppressed and the enzyme resided in high molecular mass, ribonucleoprotein complexes associated with cytoplasmic P-bodies and stress granules. Using a defined in vitro system, we show that RNA alone was sufficient to suppress A3G deaminase activity and did so in an RNA concentration-dependent manner. RNAs of diverse sequences and as short as 25 nucleotides were effective inhibitors. Native PAGE analyses showed that RNA formed ribonucleoprotein complexes with A3G and in so doing prevented ssDNA substrates from binding to A3G. The data provided direct evidence that A3G binding to cellular RNAs constituted a substantial impediment to the enzyme’s ability to interact with ssDNA. PMID:21856286

  20. Analysis of T4SS-induced signaling by H. pylori using quantitative phosphoproteomics

    Directory of Open Access Journals (Sweden)

    Frithjof eGlowinski

    2014-07-01

    Full Text Available Helicobacter pylori is a Gram-negative bacterial pathogen colonizing the human stomach. Infection with H. pylori causes chronic inflammation of the gastric mucosa and may lead to peptic ulceration and/or gastric cancer. A major virulence determinant of H. pylori is the type IV secretion system (T4SS, which is used to inject the virulence factor CagA into the host cell, triggering a wide range of cellular signaling events. Here, we used a phosphoproteomic approach to investigate tyrosine signaling in response to host-pathogen interaction, using stable isotope labeling in cell culture (SILAC of AGS cells to obtain a differential picture between multiple infection conditions. Cells were infected with wild type H. pylori P12, a P12ΔCagA deletion mutant, and a P12ΔT4SS deletion mutant to compare signaling changes over time and in the absence of CagA or the T4SS. Tryptic peptides were enriched for tyrosine (Tyr phosphopeptides and analysed by nano-LC-Orbitrap MS. In total, 58 different phosphosites were found to be regulated following infection. The majority of phosphosites identified were kinases of the MAPK familiy. CagA and the T4SS were found to be key regulators of Tyr phosphosites. Our findings indicate that CagA primarily induces activation of ERK1 and integrin linked factors, whereas the T4SS primarily modulates JNK and p38 activation.

  1. On fitting the full spectrum of luminous red galaxies by using ULySS and STARLIGHT

    International Nuclear Information System (INIS)

    We select a sample of quiescent luminous red galaxies (LRGs) from the Sloan Digital Sky Survey Data Release 7 with a high signal-to-noise ratio (S/N) to study the consistency of fitting the full spectrum by using different packages, mainly, ULySS and STARLIGHT. The spectrum of each galaxy in the sample is fitted by the full spectrum fitting packages ULySS and STARLIGHT. We find: (1) for spectra with higher S/Ns, the ages of stellar populations obtained from ULySS are slightly older than those from STARLIGHT, and metallicities derived from ULySS are slightly richer than those from STARLIGHT. In general, both packages can give roughly consistent fitting results. (2) For low S/N spectra, it is possible that the fitting by ULySS can become trapped at some local minimum in the parameter space during execution and thus may give unreliable results, but STARLIGHT can still give reliable results. Based on the fitting results of LRGs, we further analyze their star formation history and the relation between their age and velocity dispersion, and find that they agree well with conclusions from previous works

  2. Structure/property (constitutive and dynamic strength/damage characterization of additively manufactured 316L SS

    Directory of Open Access Journals (Sweden)

    Gray III G.T.

    2015-01-01

    Full Text Available For additive manufacturing (AM, the certification and qualification paradigm needs to evolve as there exists no “ASTM-type” additive manufacturing certified process or AM-material produced specifications. Accordingly, utilization of AM materials to meet engineering applications requires quantification of the constitutive properties of these evolving materials in comparison to conventionally-manufactured metals and alloys. Cylinders of 316L SS were produced using a LENS MR-7 laser additive manufacturing system from Optomec (Albuquerque, NM equipped with a 1kW Yb-fiber laser. The microstructure of the AM-316L SS is detailed in both the as-built condition and following heat-treatments designed to obtain full recrystallization. The constitutive behavior as a function of strain rate and temperature is presented and compared to that of nominal annealed wrought 316L SS plate. The dynamic damage evolution and failure response of all three materials was probed using flyer-plate impact driven spallation experiments at a peak stress of 4.5 GPa to examine incipient spallation response. The spall strength of AM-produced 316L SS was found to be very similar for the peak shock stress studied to that of annealed wrought or AM-316L SS following recrystallization. The damage evolution as a function of microstructure was characterized using optical metallography.

  3. ReSS: Resource Selection Service for National and Campus Grid Infrastructure

    International Nuclear Information System (INIS)

    The Open Science Grid (OSG) offers access to around hundred Compute elements (CE) and storage elements (SE) via standard Grid interfaces. The Resource Selection Service (ReSS) is a push-based workload management system that is integrated with the OSG information systems and resources. ReSS integrates standard Grid tools such as Condor, as a brokering service and the gLite CEMon, for gathering and publishing resource information in GLUE Schema format. ReSS is used in OSG by Virtual Organizations (VO) such as Dark Energy Survey (DES), DZero and Engagement VO. ReSS is also used as a Resource Selection Service for Campus Grids, such as FermiGrid. VOs use ReSS to automate the resource selection in their workload management system to run jobs over the grid. In the past year, the system has been enhanced to enable publication and selection of storage resources and of any special software or software libraries (like MPI libraries) installed at computing resources. In this paper, we discuss the Resource Selection Service, its typical usage on the two scales of a National Cyber Infrastructure Grid, such as OSG, and of a campus Grid, such as FermiGrid.

  4. ReSS: Resource Selection Service for National and Campus Grid Infrastructure

    Science.gov (United States)

    Mhashilkar, Parag; Garzoglio, Gabriele; Levshina, Tanya; Timm, Steve

    2010-04-01

    The Open Science Grid (OSG) offers access to around hundred Compute elements (CE) and storage elements (SE) via standard Grid interfaces. The Resource Selection Service (ReSS) is a push-based workload management system that is integrated with the OSG information systems and resources. ReSS integrates standard Grid tools such as Condor, as a brokering service and the gLite CEMon, for gathering and publishing resource information in GLUE Schema format. ReSS is used in OSG by Virtual Organizations (VO) such as Dark Energy Survey (DES), DZero and Engagement VO. ReSS is also used as a Resource Selection Service for Campus Grids, such as FermiGrid. VOs use ReSS to automate the resource selection in their workload management system to run jobs over the grid. In the past year, the system has been enhanced to enable publication and selection of storage resources and of any special software or software libraries (like MPI libraries) installed at computing resources. In this paper, we discuss the Resource Selection Service, its typical usage on the two scales of a National Cyber Infrastructure Grid, such as OSG, and of a campus Grid, such as FermiGrid.

  5. SS400钢卷冷弯不合格原因分析%Analysis on the Reason Causing Unqualified Cold Bending of SS400 Steel Coil

    Institute of Scientific and Technical Information of China (English)

    胡亚平

    2011-01-01

    通过宏观检验、力学性能测试、化学成分分析和金相检验对SS400钢卷冷弯不合格的原因进行了分析。结果表明:钢板中氧化铝夹杂偏多是冷弯试样出现裂纹的主要原因,同时提出了减少冷弯裂纹出现的措施。%By means of macroscopic examination, mechanical property testing, chemical composition analysis and metallographic examination, the reason causing unqualified cold bending property of SS400 steel coil was analyzed. The results showed that the main reason was higher content of alumina inclusion in the cold bending sample. And the measures to reduce the cracks of cold bending were given out.

  6. Tietojärjestelmän ongelmien tunnistaminen ja kehitysehdotukset eräässä osakekauppajärjestelmässä

    OpenAIRE

    Lätti, Laura

    2007-01-01

    Työ käsittelee erään pankin arvopaperikaupankäynnissä käytettävää järjestelmää. Työn tarkoituksena on parantaa järjestelmän tukemaa dealing-liiketoimintaa mahdollisimman kustannustehokkaasti. Työssä keskitytään järjestelmän ongelmien ja niiden syiden selvittämiseen sekä mahdollisten kehitysehdotuksien laatimiseen. Työn kirjallisuusosiossa tutustutaan yleisesti tietojärjestelmiin sekä järjestelmäintegraatioon, sillä työssä kyseessä oleva järjestelmä koostuu useista osajärjestelmistä. Lisäk...

  7. Kyllin hyvä työssäoppimisorganisaatio : -näkökulmia työssäoppimisen organisaatiolähtöisiin tekijöihin

    OpenAIRE

    Väyrynen, Outi

    2010-01-01

    Opinnäytetyössä tarkastellaan työssäoppimista sosiaali- ja terveysalan organisaatiolähtöisten tekijöiden näkökulmasta. Lähtökohtana on ollut tutkia konkreettisia tekijöitä, joilla organisaatiot pystyvät kehittämään ja luomaan kyllin hyviä työssäoppimisorganisaatioita: miten käytännössä päästään laadukkaaseen työssäoppimisprosessiin ja millaisia toimivia työmenetelmiä organisaatioissa on kehitetty, jotta työssäoppimisen tavoitteisiin päästäisiin. Työpaikan oppimiskulttuuri- ja käytäntö, r...

  8. Asymmetric PCR method in generation of HBV ssDNA for pyrosequencing

    Institute of Scientific and Technical Information of China (English)

    Nian-cai Peng; Chun-lin Wang; Li-li Zhang; Mao-li Lu; Zhen-xi Zhang

    2009-01-01

    Objective To explore the optimal primer ratio and concentration of asymmetric polymerase chain reaction (A-PCR) in producing hepatitis B virus (HBV) single-stranded DNA (ssDNA) for pyrosequencing. Methods A-PCR was carried out to generate HBV ssDNA with forward to reverse primers of different ratios (50 : 1, 100 : 1) and concentrations (13. 0 pmol/25μL and 0.14 pmol/25μL, 19. 5 pmol/25μL and 0. 21 pmol/25μL), and the product yield and quality were compared respectively. Results The forward to reverse primer ratio of 50 : 1 provided better yield and concentration of 19. 5 pmol/25μL and 0. 21 pmol//25μL generated a clearer band. Conclusion A simple and feasible method to produce HBV ssDNA for pyrosequencing in batch is established.

  9. 3D-hahmon luonti ja ohjaaminen Cave-virtuaaliympäristössä

    OpenAIRE

    Orava, Peetu

    2011-01-01

    ssä työssä on perehdytty 3D-hahmon luonnin eri vaiheisiin, joihin kuuluu suunnittelu Photoshopissa, mallinnus 3ds Maxissa, yksityiskohtien ja tekstuurien luonti ZBrushissa. Mallinnetulle hahmolle on myös luotu Biped-luujärjestelmä 3ds Maxissa Skin-muokkaimen kanssa. Tämän jälkeen valmis hahmo on viety Virtools-ohjelmaan ja siinä luotu hahmolle kontrollit peliohjaimelle, jotta sitä voi ohjata Cave-virtuaaliympäristössä käyttäen valmiita animaatioita. Samalla käydään läpi työnkulun eri v...

  10. Henkilöstön motivoinnin ja sitouttamisen keinoja esimiestyössä

    OpenAIRE

    Lahtonen-Pääkkönen, Katja; Salohalme, Tommi

    2015-01-01

    ssä opinnäytetyössä on tarkasteltu keinoja henkilöstön motivoimiseen ja sitouttamiseen esimiestyössä. Opinnäytetyön tärkeimpänä tavoitteena oli löytää konkreettisia keinoja ja menetelmiä kollegoille sekä yrityksien henkilöstölle käytettäväksi henkilöstön motivoimiseksi ja sitouttamiseksi. Tämän lisäksi tarkoituksena oli kehittyä itse ammatillisesti ihmisten johtajana. Käsillä oleva opinnäytetyö rakentuu teoreettisista sekä empiirisista luvuista. Tutkimuksen teoreettinen viitekehys koo...

  11. Nucleation and growth microstructural study of ti films on 304 SS substrates

    Directory of Open Access Journals (Sweden)

    Rogério de Almeida Vieira

    2004-09-01

    Full Text Available Coating of steel surfaces with titanium films has been studied with the objective to protect them against corrosion, and to create an intermediate film for CVD diamond and TiN film deposition. In this work, the nucleation, growth mechanisms and microstructural formation of the titanium films deposited on 304 stainless steel (304 SS substrate are presented and discussed. The titanium films of variable thickness were obtained by vapour phase deposition produced by electron beam. The surfaces of these samples were observed by scanning electron microscopy. The cross sections of these samples were observed by using an atomic force microscope. The Ti film-304 SS interfaces were analyzed by X-ray diffraction. The results showed that titanium films have a columnar growth. The Ti film-304 SS interface had a residual compression stress at room temperature due to the inter-diffusion process.

  12. Intranetin rooli asuinyhteisön viestinnässä

    OpenAIRE

    Hakkarainen, Veera

    2012-01-01

    Tämän opinnäytetyö käsittelee Arabian Palvelu Oy:n asiakastaloyhtiöiden käytössä ole-via intranetsivuja eli taloyhtiösivuja. Tutkimuksessa selvitetään niiden tarpeellisuutta ja käyttöä taloyhtiöissä ja kartoitetaan palvelun kehitystarvetta. Työssä selvitetään asuinyhteisön sosiaalisia rakenteita ja viestinnällisiä tarpeita yleisem-mällä tasolla, sekä sitä, kuinka intranet pystyy vastaamaan näihin tarpeisiin. Työssä kar-toitetaan intranetin roolia sen heikkouksien ja vahvuuksien kautta sek...

  13. Stability of strange stars (SS) derived from a realistic equation of state

    CERN Document Server

    Sinha, M N; Dey, M; Ray, S; Bhowmick, S; Sinha, Monika; Dey, Jishnu; Dey, Mira; Ray, Subharthi; Bhowmick, Siddhartha

    2002-01-01

    A realistic equation of state (EOS) leads to realistic strange stars (ReSS) which are compact in the mass radius plot, close to the Schwarzchild limiting line (Dey et al 1998). Many of the observed stars fit in with this kind of compactness, irrespective of whether they are X-ray pulsars, bursters or soft $\\gamma$ repeaters or even radio pulsars. We point out that a change in the radius of a star can be small or large, when its mass is increasing and this depends on the position of a particular star on the mass radius curve. We carry out a stability analysis against radial oscillations and compare with the EOS of other strange star (SS) models. We find that the ReSS is stable and an M-R region can be identified to that effect.

  14. Resistance of superhydrophobic and oleophobic surfaces to varied temperature applications on 316L SS

    Science.gov (United States)

    Shams, Hamza; Basit, Kanza; Saleem, Sajid; Siddiqui, Bilal A.

    316L SS also called Marine Stainless Steel is an important material for structural and marine applications. When superhydrophobic and oleophobic coatings are applied on 316L SS it shows significant resistance to wear and corrosion. This paper aims to validate the coatings manufacturer's information on optimal temperature range and test the viability of coating against multiple oil based cleaning agents. 316L SS was coated with multiple superhydrophic and oleohobic coatings and observed under SEM for validity of adhesion and thickness and then scanned under FFM to validate the tribological information. The samples were then dipped into multiple cleaning agents maintained at the range of operating temperatures specified by the manufacturer. Coating was observed for deterioration over a fixed time intervals through SEM and FFM. A comparison was drawn to validate the most critical cleaning agent and the most critical temperature at which the coating fails to leave the base substrate exposed to the environment.

  15. Harjoituskäytössä olevien nestesammuttimien huolto-ohjeet

    OpenAIRE

    Nummela, Juuso

    2014-01-01

    Tämän opinnäytetyön lähtökohtana oli Turvallisuus ja kemikaalivirastolle, Tukesille, tulleet toistuvat kyselyt kuinka harjoituskäytössä olevien nestetäytteisten sammuttimien huolto tulisi toteuttaa. Työ toteutettiin perehtymällä eri toimijoiden huoltokäytäntöihin harjoituskäytössä olevien sammuttimien osalta, sekä selvitin kuinka lainsäädäntö ja muu normisto ohjeistavat harjoituskäytössä olevien sammuttimien huoltoa. Huoltokäytäntöjä on selvitetty haastattelemalla eri toimijoita joilla o...

  16. Serum testosterone levels of HbSS (sickle cell disease male subjects in Lagos, Nigeria

    Directory of Open Access Journals (Sweden)

    Adediran Adewumi

    2011-08-01

    Full Text Available Abstract Background Infertility is a major problem in sickle cell disease patients, especially in males. In addition to low serum testosterone, other abnormalities involving the accessory sex organs, such as the seminal vesicles and the prostate gland, as well as marked decrease in ejaculate volume may be observed in male HbSS patients. Hence, the need to study the role of sex hormones as a cause of infertility in male HbSS patients. Methods An unmatched case-control study was performed using seventy-five consenting subjects from Lagos University Teaching Hospital. These included 47 patients with haemoglobin phenotype SS from the Sickle cell clinic and 28 volunteered medical students and members of staff with haemoglobin phenotype AA. Demographic data were obtained using a self-administered questionnaire. A total of 5 mls of blood was collected from each subject between 9.00 am & 11.am, and assayed for serum testosterone concentration. Results The concentrations of serum testosterone in HbSS patients ranged from 0.2 to 4.3 ng/ml with a mean of 1.28 ± 0.72 ng/ml whilst the values in HbAA controls ranged from 1.2 to 6.9 ng/ml with a mean of 2.63 ± 1.04 ng/ml. Seven (25.0% of the 28 controls had serum testosterone concentration lower than the quoted reference (normal range whereas 44 (93.6% of the 47 HbSS subjects had serum testosterone concentration lower than the reference range. Conclusion Overall, subjects with HbSS have significantly lower mean serum testosterone than HbAA controls.

  17. Binding hot-spots in an antibody-ssDNA interface: a molecular dynamics study.

    Science.gov (United States)

    Wang, Yeng-Tseng; Lee, Wen-Jay

    2012-10-30

    Simulating antigen-antibody interactions is essential for elucidating antigen-antibody mechanics. Proteins interactions are vital for elucidating antibody-ssDNA associations in immunology. Therefore, this study investigated the dissociation of the human systemic lupus erythematosus antibody-ssDNA complex structure. Dissociation (i.e. the distance between the center of mass of the ssDNA and the antibody) is also studied using the potential of mean force calculations based on molecular dynamics and the explicit water model. The MM-PBSA method is also used to prove our dissociation simulations. With 605 nanosecond molecular dynamics simulations, the results indicate that the 8 residues (i.e. Gly44 (HCDR2), Asn54 (HCDR2), Arg98 (HCDR3), Tyr100 (HCDR3), Asp101 (HCDR3), Tyr32 (LCDR1), Tyr49 (LCDR2) and Asn50 (LCDR2)), and the five inter-protein molecular hydrogen bonds may profoundly impact the antibody-ssDNA interaction, a finding which may be useful for protein engineering of this antibody-ssDNA structure. Experimental binding affinity of this antibody-ssDNA complex equals 7.00 kcal mol(-1). Our dissociation binding affinity is 7.96 ± 0.33 kcal mol(-1) and MM-PBSA binding affinity is 9.12 ± 1.65 kcal mol(-1), which is close to the experimental value. Additionally, the 8 residues Gly44 (HCDR2), Asn54 (HCDR2), Arg98 (HCDR3), Tyr100 (HCDR3), Asp101 (HCDR3), Tyr32 (LCDR1), Tyr49 (LCDR2) and Asn50 (LCDR2) may play a more significant role in developing bioactive antibody analogues. PMID:23079742

  18. Cyclic behavior of SS 316 LN with respect to thermal ratcheting of main vessel for SFR

    International Nuclear Information System (INIS)

    One of the innovative features which are considered for the future Sodium cooled Fast breeder Reactor (SFR) in India is the elimination of main vessel cooling circuit. This would bring out significant economic by saving the cost of entire cooling circuit and also by reducing the main vessel diameter. However, the challenging aspect to be addressed is investigation of thermal ratcheting failure near sodium free level. The sodium free level will vary during the various operating conditions depending up on hot and cold pool temperatures. The thermal fluctuations cause high cycle deformation controlled fatigue damage. These high cycle temperature oscillations cause high cycle deformation controlled fatigue on metal surface. Thermal ratcheting of main vessel enhanced by creep effect which cause radial deformations near the sodium free level during Safety Graded Decay Heat Removal (SGDHR). The strain accumulated due to thermal ratcheting can also cause reduction in buckling strength of main vessel. The accumulated deformations can cause concern for the free movement of in-service inspection equipment. Preliminary assessment indicates that there is a need to develop special SS 316 LN with higher nitrogen content and subsequently to demonstrate that the vessel made of this material can provide a sufficient safety margin against ratcheting. This exercise calls for carrying out many simulated experiments, particularly to predict thermal ratcheting. Some of the recent bi-axial tension-torsion experiments to simulate ratcheting for SS316LN and enhanced nitrogen steel (SS316EN) found that the axial strains predicted by enhanced nitrogen SS316EN steel were much higher than the strains predicted for SS316LN steel for the same set of parameters. Hence it is worthwhile to carryout investigations to predict the cyclic behavior of SS 316LN and enhanced nitrogen steel with respect to thermal ratcheting. Some experiments have been conducted for the SFR scale down model of main vessel

  19. Effects of inelastic (re)scattering processes ss-bar → gg and gg → ss-bar on strange hadron production in pp collisions at RHIC and LHC energies

    International Nuclear Information System (INIS)

    The rapidity densities at mid-rapidity and the transverse momentum distributions for strange hadrons produced in pp collisions are analyzed using the modified PACIAE model by considering the effect of inelastic (re)scattering processes ss-bar → gg and gg → ss-bar in parton (re) scattering. The calculated results of the transverse momentum spectra of the strangeness fitting with data measured by SIFAR and ALICE Collaborations can be improved, especially at large transverse momentum levels. This demonstrates that the effect of inelastic (re)scattering processes of ss-bar → gg and gg → ss-bar is not negligible at RHIC and LHC energy levels. (authors)

  20. Exploring optimization parameters to increase ssDNA recombineering in Lactococcus lactis and Lactobacillus reuteri

    OpenAIRE

    van Pijkeren, Jan-Peter; Neoh, Kar Mun; Sirias, Denise; Findley, Anthony S.; Britton, Robert A.

    2012-01-01

    Single-stranded DNA (ssDNA) recombineering is a technology which is used to make subtle changes in the chromosome of several bacterial genera. Cells which express a single-stranded DNA binding protein (RecT or Bet) are transformed with an oligonucleotide which is incorporated via an annealing and replication-dependent mechanism. By in silico analysis we identified ssDNA binding protein homologs in the genus Lactobacillus and Lactococcus lactis. To assess whether we could further improve the r...

  1. Työssäoppimisjaksojen kehittäminen oppaiden avulla : Helsingin kaupungin liikuntaviraston liikunnanohjausosastolla

    OpenAIRE

    Hakkarainen, Jarna

    2014-01-01

    Opinnäytetyöprojektin tarkoituksena oli luoda Helsingin kaupungin liikuntaviraston liikunnanohjausosaston liikunnanohjaajien käyttöön opas työssäoppijoiden ohjaami-seen. Opas on nimensä mukaan apuväline liikunnanohjaajille opiskelijoiden ohjaami-seen. Toisena oppaana valmistui työpaikkaohjaajien sekä opiskelijoiden käyttöön Ter-vetuloa työssäoppimisjaksolle Helsingin kaupungin liikuntaviraston liikunnanohjaus-osastolle – opas. Tervetuloa – oppaan tarkoituksena on perehdyttää opiskelija liikun...

  2. Työelämässä selviytymisen huomioiminen nivelreumakursseilla

    OpenAIRE

    Lehtonen, Jari

    2015-01-01

    Opinnäytetyössä kartoitin työryhmän tapoja huomioida työelämässä jaksamista nivelreumaa sairastavien kuntoutuskurssien toteutuksessa. Kuntoutuskeskus Kankaanpää toimii yhtenä palveluntuottajana Kelan järjestämille harkinnanvaraisille sairausryhmäkursseille. Nivelreumaa sairastavien kuntoutuskurssia toteutetaan 2013-2016 standardikauden mukaisesti. Standardi ohjaa kuntoutuksen toteutusta, rakennetta ja käytettävää henkilöstöä. Kuntoutuksen sisältö rakentuu kuntoutujien tavoitteita tukevasti. T...

  3. Kehitysvammaisuus suomalaisessa elokuvataiteessa 1920-luvulta vuoteen 2010 : Yhteiskunnallisia merkityksiä etsimässä

    OpenAIRE

    Markus, Helena

    2011-01-01

    Tutkimustyöni lähtökohtana on sosionomin tehtäväkentässä kulttuurillisuuden vahvistaminen. Kehitysvammaisuus suomalaisessa elokuvataiteessa – yhteiskunnallisia merkityksiä etsimässä, on lyhyt katsaus kotimaisesta elokuvasta 1920-luvulta nykypäivään. Tavoitteenani on etsiä vuosikymmenittäin elokuvia, joissa on kehitysvammaiseksi henkilöksi luonnehdittava hahmo roolisuorituksena. Löytämistäni elokuvista olen analysoinut sosiaalipoliittisten tavoitteiden, asenteiden ja arvojen näkymistä kullakin...

  4. Printtitoimittaja luovii etiikan viidakossa alusta loppuun : Ammattietiikka toimittajan työssä

    OpenAIRE

    Eriksson, Susanna

    2011-01-01

    TIIVISTELMÄ Eriksson, Susanna 2011. Printtitoimittaja luovii etiikan viidakossa alusta loppuun. Am-mattietiikka toimittajan työssä. Opinnäytetyö. Kemi-Tornion ammattikorkeakoulu. Kulttuuriala. Viestinnän koulutusoh-jelma. Sivuja 37. Liitteitä 1. Opinnäytetyöni aiheena on ammattietiikan näkyminen kirjoittavan sanomalehtitoimitta-jan jokapäiväisessä työssä. Halusin selvittää eritoten sitä, miten iän tuoma kokemus sekä tiedot ja taidot vaikuttavat toimitusprosessin aikana tehtävii...

  5. Sosiaalisen median mahdollisuudet markkinointi- ja asiakasviestinnässä - tapaus Yritys Oy

    OpenAIRE

    Mikkonen, Kirsi

    2012-01-01

    Yritysviestinnän näkökulmasta sosiaalinen media on mielenkiintoinen sekoitus viestintää, markkinointia ja asiakaspalvelua. Tämän opinnäytetyön aiheena on sosiaalisen median hyödyntäminen yrityksen markkinointi- ja asiakasviestinnässä. Tarkoituksena oli selvittää, millaisia sosiaalisen median työkaluja toimeksiantaja Yritys Oy voisi käyttää ulkoisessa viestinnässään. Tutkimusmenetelmänä oli kirjallisuuskatsaus, johon sisällytettiin viestinnän, markkinoinnin ja sosiaalisen median kirjallisuu...

  6. Toimintaterapia-arviointi osana kuntoutuksen suunnittelua kuntoutustyöryhmässä

    OpenAIRE

    Rantanen, Sanna; Pehkonen, Riitta

    2011-01-01

    ssä opinnäytetyössä tavoitteena oli löytää sopivia,näyttöön perustuvia ja moniammatillisesti hyödynnettäviä toimintaterapian arviointimenetelmiä Pohjois-Karjalan sairaanhoito- ja sosiaalipalvelujen kuntayhtymän (PKSSK) aikuispsykiatrian poliklinikan kuntoutustyöryhmän käyttöön. Opinnäytetyön tutkimus oli kvalitatiivinen ja se toteutettiin ryhmämuotoisella teemahaastattelulla, sekä kirjallisuuskatsauksella. Kuntoutustyöryhmän haastattelussa selvitettiin PKSSK:n aikuispsykiatrian poliklinikan...

  7. Suklaajälkiruoat ja niiden valmistamisen haasteet ammattikeittiössä

    OpenAIRE

    Lappalainen, Arto

    2016-01-01

    Tämä opinnäytetyö on osa palvelujen tuottamisen ja johtamisen koulutusohjelmaa. Opinnäytetyön aiheena on suklaajälkiruuat ja niiden valmistamisen haasteet ammattikeittiössä. Opinnäytetyön teoriaosuudessa käsitellään suklaan historiaa, lajikkeita, tuottajamaita, valmistusmenetelmiä, suklaan laatuja, tärkeimpiä valmistajia sekä suklaan käyttöä ja tekniikoita ammattikeittiössä. Tutkimuksessa selvitettiin yleisimpiä suklaajälkiruokia ja niiden valmistuksessa olevia haasteita ammattikeitti...

  8. Ihminen ihmiselle : Ohje hoitajille kuolevan potilaan kohtaamiseen hoitotyössä

    OpenAIRE

    Jäntti, Riitta; Rautiainen, Jaana

    2015-01-01

    Toiminnallisessa opinnäytetyössä tehtiin hoitajille tarkoitettu ohje Kuopion kaupungin Harjulan sairaalan saattohoitoyksikköön. Toiminnallisena tehtävänä oli tuottaa hoitohenkilökunnalle ohje, joka sisältää keskeisimpiä lähestymistapoja kuolevan potilaan hoitotyössä. Kehittämistyö pohjautuu uusimpiin saattohoitosuosituksiin sekä Englannissa 1990 -luvun alussa kehitettyyn ” One change to get right”- asiakirjaan, jossa on lueteltu lähestymistapoja saattohoidon suositusten tueksi. Työn tavoittee...

  9. Asymmetric PCR method in generation of HBV ssDNA for pyrosequencing

    Institute of Scientific and Technical Information of China (English)

    2009-01-01

    Objective To explore the optimal primer ratio and concentration of asymmetric polymerase chain reaction (A-PCR) in producing hepatitis B virus (HBV) single-stranded DNA (ssDNA) for pyrosequencing. Methods A-PCR was carried out to generate HBV ssDNA with forward to reverse primers of different ratios (50∶1, 100∶1) and concentrations (13.0 pmol/25μL and 0.14 pmol/25μL, 19.5 pmol/25μL and 0.21 pmol/25μL), and the product yield and quality were compared respectively. Results The forward to reverse primer ratio ...

  10. Energy Efficiency Experiments on Samsung Exynos 5 Heterogeneous Multicore using OmpSs Task Based Programming

    OpenAIRE

    Holmgren, Rune

    2015-01-01

    This thesis explore the energy efficiency of task based programming with OpenMP SuperScalar (OmpSs) on the heterogeneous Samsung Exynos 5422 system on a chip. The system features small energy efficient cores, large high performance cores and a GPGPU, and OmpSs tasks were run on all three different processors. Experiments running a genetic algorithm and a Cholesky decomposition were used to gather results. The option of running applications on the energy efficient cores, on the high perfo...

  11. Taekwon-do : Sosiaalinen tuki vanhempien kasvatustehtävässä

    OpenAIRE

    Kangasniemi, Tanja

    2012-01-01

    Tämän opinnäytetyön päätarkoituksena on selvittää Taekwon-Don antamaa kasvatuskumppanuutta ja sosiaalista tukea vanhemmille. Minkälaista sosiaalista tukea Taekwon-Don pääperiaatteet ja filosofia voivat tuoda vanhempien kasvatustehtävään? Taekwon-Do filosofia pohjautuu lajin kehittäjän kenraali Choi Hong Hi:n kirjoittamiin periaatteisiin, elämänfilosofiaan ja protokollaan. Pääperiaatteet ovat: rehellisyys, kohteliaisuus, uutteruus, itsehillintä ja lannistumaton henki. Tässä työssä Taekwon-...

  12. Ylisukupolvisuus toimeentulotukityössä : sosiaaliohjaajien työskentely perheiden kanssa

    OpenAIRE

    Meling, Maiju; Strellman, Merja

    2015-01-01

    TIIVISTELMÄ Meling, Maiju & Strellman, Merja. Ylisukupolvisuus toimeentulotukityössä. Sosiaaliohjaajien työskentely perheiden kanssa. Diak, Helsinki, kevät 2015, 76 s., 4 liitettä. Diakonia-ammattikorkeakoulu, Sosiaalialan koulutusohjelma, Päihteet ja syrjäytyminen, sosionomi (YAMK). Opinnäytetyön tavoitteena on kartoittaa toimeentulotukiasiakkuuden ylisukupolvisuutta ilmiönä ja aikuissosiaalityön asiakasperheiden kanssa tehtävää työtä sosiaaliohjauksen näkökulmasta. Opinnäytetyöss...

  13. Perehdyttäminen Lastenlinnan video-EEG-yksikössä

    OpenAIRE

    Junnonen, Satu

    2009-01-01

    TIIVISTELMÄ Junnonen, Satu. Perehdyttäminen Lastenlinnan video-EEG-yksikössä. Helsinki, syksy 2009, 77 s., 2 liitettä. Diakonia-ammattikorkeakoulu, Diak Etelä, Helsinki. Hoitotyön koulutusohjelma, Hoitotyön suuntautumisvaihtoehto, muuntokoulutus, sairaanhoitaja (AMK). Opinnäytetyöni on produktio. Sen tarkoituksena oli laatia kirjallinen perehdytyskansio epilepsiaosaston hoitajille video-EEG-yksikössä työskentelystä. Video-EEG ja epilepsiaosasto LL11 kuuluvat molemmat Helsingin Las...

  14. Esimiehenä tiimissä : kirjallisuuskatsaus tiimin johtajuudesta hoitotyössä

    OpenAIRE

    Hakuli, Leena

    2015-01-01

    Tämän tutkimuksen tavoitteena oli saada tietoa siitä, millaista johtajuutta tiimi-työskentely hoitotyössä edellyttää, jotta se olisi onnistunutta ja tiimin toimintaa tukevaa. Tavoitteena oli myös selvittä onko tiimityön johtamisesta hoitotyössä tehdyissä tutkimuksissa noussut esiin johtajuuden muuttuminen verrattuna aikaisempiin johtajuus tutkimuksiin. Aineisto haettiin Medic- ja Cinahl-tietokannoista sekä manuaalisesti Hoitotiede-, Premissi- ja Tutkiva Hoitotyö –lehdistä sekä suomalaiste...

  15. Tiimityön toimivuus päivystyshoitotyössä sairaanhoitajien kokemana

    OpenAIRE

    Lahdenperä, Ville; Märijärvi, Janne

    2012-01-01

    Lahdenperä, Ville & Märijärvi, Janne. Tiimityön toimivuus päivystyshoitotyössä sairaanhoitajien kokemana. Helsinki, Kevät 2012, 59 s, 4 liitettä. Diakonia-ammattikorkeakoulu, Diak Etelä, Helsinki. Sosiaali- ja terveysalan koulutusohjelma, hoitotyön suuntautumisvaihtoehto, sairaanhoitaja (AMK). Tämän opinnäytetyön tarkoitus on kuvata, miten Peijaksen sairaalan päivystysalueella työskentelevät sairaanhoitajat kokevat moniammatillisen tiimityön toimivan päivystyshoitotyössä. Työn tarkoit...

  16. Ratsastuskilpailukäytössä olevien kenttien ja maneesien pohjaolosuhteet Suomessa

    OpenAIRE

    Eerikäinen, Milja

    2015-01-01

    Työn tavoitteena oli kerätä tietoa ratsastuskilpailukäytössä olevien pohjien ominaisuuksista ja niiden merkityksestä turvallisuuteen ja hevosen suori-tuskykyyn pohjien arvioinnin tueksi, sekä kartoittaa Suomen ratsastuskil-pailukäytössä olevien pohjien rakenteita, olosuhteita ja huoltomenetelmiä. Tämän työn toimeksiantajana toimi Suomen Ratsastajainliitto Ry. Työn teoreettisessa osiossa käsitellään pohjien ominaisuuksia ja niiden vaikutuksia turvallisuuteen ja hevosen suorituskykyyn. Tut...

  17. Maailma kylässä 2014 -festivaali: Näytteilleasettajatutkimus

    OpenAIRE

    Stylman, Maija

    2014-01-01

    Opinnäytetyön aiheena on Maailma kylässä 2014 –festivaalin näytteilleasettajatutkimus. Määrällisen tutkimuksen tarkoituksena oli selvittää näytteilleasettajien kokemuksia ja mielipiteitä festivaalista. Tutkimuksen tavoitteena oli löytää ideoita, joilla voisi kehittää festivaalin näytteilleasettajatoimintaa seuraavana vuonna. Tutkimuskysymyksiä oli kolme: miksi näytteilleasettajat osallistuivat Maailma kylässä –festivaalille tänä vuonna, miten he arvioivat festivaalin onnistumista omasta näkök...

  18. Gospel-lattarit työvälineenä kirkon nuorisotyössä

    OpenAIRE

    Joutsiniemi, Johanna

    2012-01-01

    Halusin työssäni tutkia ja avata Gospel-lattareiden hyötyjä hyvinvoinnin kannalta ajateltuna kasvatustyössä. Toiminnallisen osuuden kautta halusin tarjota nuorille mahdollisuuden tutustua Gospel-lattareihin ja kokeilla niitä lyhyiden Gospel-lattarituokioiden muodossa. Tarkoitukseni oli myös madaltaa kynnystä lähteä ehkä myöhemmin harrastamaan tanssimista, niiden kohdalla joille tanssiminen ei ollut entuudestaan niin tuttua. Halusin lisäksi tuoda ideoita kirkon nuorisotyöhön tarjoten esimerkke...

  19. Tutkimus työturvallisuudesta KerabitPro Oy:ssä

    OpenAIRE

    Savinainen, Kimmo

    2013-01-01

    Opinnäytetyössä tutkittiin työturvallisuuden tasoa erityisesti yrityksen pää-urakoimilla työmailla. Tutkimus tehtiin eri puolella Suomea 5:llä eri alueella. Turvallisuustason mittaukseen käytettiin talonrakennus eli TR-mittausta, koska se on yrityksessä muutoinkin käytössä. Tämä mahdollisti tulosten vertailun yrityksen sisäisiin mittauksiin. Tutkimus tehtiin loka-marraskuun 2012 aikana ja siinä käytiin läpi yhteensä 25 työmaata. Kokonaisuutena työturvallisuuden taso oli hyvä, mutta poikk...

  20. Rakennustyömaan laadunvarmistus : Rakennustyöt Ville Kauppi Oy:ssä

    OpenAIRE

    Penttilä, Ilmari

    2016-01-01

    Tämän työn tavoitteena oli luoda laadunvarmistusdokumentteja ja -menetelmiä Rakennustyöt Ville Kauppi Oy:lle. Työssä tehtiin yrityksen tarpeisiin laadunvarmistusdokumentteja ja muistilistoja työmaatason laadunvarmistusta varten ja niiden avulla yritetään kehittää yrityksen sisäisiä toimintamalleja laadukkaammiksi. Osa lomakkeista on muokattu yleisesti käytössä olevista laadunvarmistusdokumenteista yrityksen tarpeisiin paremmin sopiviksi. Luodut dokumentit ladattiin yrityksen sisäiseen pi...

  1. Pienyrityksen haasteet markkinointiviestinnässä : case: Moremix (Noworries Oy)

    OpenAIRE

    Laakso, Mari

    2009-01-01

    Tämä opinnäytetyö käsittelee markkinointiviestintää ja sen toteutusta pienyrityksessä. Tavoitteena on selvittää, mitä haasteita markkinointiviestinnässä kohdataan ja kuinka niihin on pystytty vastaamaan. Tutkimuksesta selviää myös, miten markkinointiviestintää on kehitetty yrityksessä. Teoriaosuudessa tarkastellaan markkinointiviestintää käsitteenä sekä sen tavoitteita osana markkinointia. Tutkimuksessa käsitellään integroinnin tärkeyttä viestinnässä ja sen vaikutusta viestinnän tehokkuut...

  2. Truncated SSX protein suppresses synovial sarcoma cell proliferation by inhibiting the localization of SS18-SSX fusion protein.

    Directory of Open Access Journals (Sweden)

    Yasushi Yoneda

    Full Text Available Synovial sarcoma is a relatively rare high-grade soft tissue sarcoma that often develops in the limbs of young people and induces the lung and the lymph node metastasis resulting in poor prognosis. In patients with synovial sarcoma, specific chromosomal translocation of t(X; 18 (p11.2;q11.2 is observed, and SS18-SSX fusion protein expressed by this translocation is reported to be associated with pathogenesis. However, role of the fusion protein in the pathogenesis of synovial sarcoma has not yet been completely clarified. In this study, we focused on the localization patterns of SS18-SSX fusion protein. We constructed expression plasmids coding for the full length SS18-SSX, the truncated SS18 moiety (tSS18 and the truncated SSX moiety (tSSX of SS18-SSX, tagged with fluorescent proteins. These plasmids were transfected in synovial sarcoma SYO-1 cells and we observed the expression of these proteins using a fluorescence microscope. The SS18-SSX fusion protein showed a characteristic speckle pattern in the nucleus. However, when SS18-SSX was co-expressed with tSSX, localization of SS18-SSX changed from speckle patterns to the diffused pattern similar to the localization pattern of tSSX and SSX. Furthermore, cell proliferation and colony formation of synovial sarcoma SYO-1 and YaFuSS cells were suppressed by exogenous tSSX expression. Our results suggest that the characteristic speckle localization pattern of SS18-SSX is strongly involved in the tumorigenesis through the SSX moiety of the SS18-SSX fusion protein. These findings could be applied to further understand the pathogenic mechanisms, and towards the development of molecular targeting approach for synovial sarcoma.

  3. Strategia vanginvartijan työssä : Turun vankilan vanginvartijoiden näkemyksiä Rikosseuraamuslaitoksen strategiasta 2011 – 2020

    OpenAIRE

    Granith, Linda; Koskenvuori, Enni

    2013-01-01

    Opinnäytetyössä tutkitaan Rikosseuraamuslaitoksen strategian 2011 – 2020 näkymistä Turun vankilan vanginvartijoiden jokapäiväisessä työssä. Opinnäytetyössä tutkitaan Rikosseuraamuslaitoksen strategian käsittelyä ja tiedottamista työyhteisöissä sekä sitä, ovatko vanginvartijat tutustuneet strategiaan. Strategian vaikutusta vanginvartijoiden työhön käsitellään strategiaan kirjattujen arvojen sekä vanginvartijan työhön liittyvien tavoitteiden kautta. Työssä nostetaan esiin vanginvartijoiden näke...

  4. Atividade clastogênica e genotóxica de altas concentrações do extrato aquoso de Agaricus brasiliensis e diferentes respostas quando associado aos inibidores de reparo de DNA, ARA-C e 3DEOT, in vitro.

    Directory of Open Access Journals (Sweden)

    Mario Sergio Mantovani

    2006-05-01

    Full Text Available O Agaricus brasiliensis Wasser & Didukh – Ab (=Agaricus blazei Murrill ss. Heinemann é um basidiomiceto que vem sendo consumido em diversas partes do mundo no combate e tratamento de muitas doenças. Neste estudo, foram testados, em células de ovário de hamster chinês (CHO-k1, os efeitos clastogênicos e genotóxicos de altas concentrações de Ab e seu potencial protetor, por meio dos ensaios de aberração cromossômica (AC e cometa (SCGE, associados a dois bloqueadores de reparo do DNA (citosina arabinoside trifosfato - Ara-C - inibidor de DNA polimerase a e 3’deoxitimidina 5’trifosfato - 3DeoT - inibidor de DNA polimerase b, na presença ou não de um agente alquilante (metilmetanosulfonato. No teste de clastogenicidade, verificou-se que as concentrações 0,2 e 0,4% não se mostraram indutoras de dano, ao contrário da maior concentração (0,6%. Nos tratamentos de genotoxicidade no SCGE, a concentração de 0,2% do extrato não mostrou atividade genotóxica, ao contrário das concentrações de 0,4 e 0,6%, as quais foram efetivas indutoras de danos no DNA. Os resultados de anticlastogenicidade indicaram que, na maioria dos tratamentos realizados, o extrato aquoso de Ab não apresentou atividade protetora contra danos no DNA, induzidos pela Ara-C e Ara-C + MMS. Pelo SCGE, Ab, nas três concentrações testadas, não mostrou atividade antigenotóxica. Os dados sugerem cuidado no consumo e ingestão de Ab por seres humanos, principalmente em altas concentrações.

  5. Screening and Identification of ssDNA Aptamer for Human GP73

    Directory of Open Access Journals (Sweden)

    Jingchun Du

    2015-01-01

    Full Text Available As one tumor marker of HCC, Golgi Protein 73 (GP73 is given more promise in the early diagnosis of HCC, and aptamers have been developed to compete with antibodies as biorecognition probes in different detection system. In this study, we utilized GP73 to screen specific ssDNA aptamers by SELEX technique. First, GP73 proteins were expressed and purified by prokaryotic expression system and Nickle ion affinity chromatography, respectively. At the same time, the immunogenicity of purified GP73 was confirmed by Western blotting. The enriched ssDNA library with high binding capacity for GP73 was obtained after ten rounds of SELEX. Then, thirty ssDNA aptamers were sequenced, in which two ssDNA aptamers with identical DNA sequence were confirmed, based on the alignment results, and designated as A10-2. Furthermore, the specific antibody could block the binding of A10-2 to GP73, and the specific binding of A10-2 to GP73 was also supported by the observation that several tumor cell lines exhibited variable expression level of GP73. Significantly, the identified aptamer A10-2 could distinguish normal and cancerous liver tissues. So, our results indicate that the aptamer A10-2 might be developed into one molecular probe to detect HCC from normal liver specimens.

  6. Ball Indentation Studies on the Effect of Nitrogen on the Tensile Properties of 316LN SS

    Science.gov (United States)

    Mathew, M. D.; Ganesh Kumar, J.; Ganesan, V.; Laha, K.

    2015-12-01

    Type 316L(N) stainless steel (SS) containing 0.02-0.03 wt% carbon and 0.06-0.08 wt% nitrogen is used as the major structural material for the components of fast reactors. Research is underway to improve the high-temperature mechanical properties of 316LN SS by increasing the nitrogen content in the steel above the level of 0.08 wt%. In this investigation, ball indentation (BI) technique was used to evaluate the effect of nitrogen content on the tensile properties of 316LN SS. BI tests were conducted on four different heats of 316LN SS containing 0.07, 0.11, 0.14 and 0.22 wt% nitrogen in the temperature range 300-923 K. The tensile properties such as yield strength and ultimate tensile strength increased with increase in nitrogen content at all the investigated temperatures. These results were consistent with the corresponding uniaxial tensile test results. These studies showed that BI technique can be used to optimize the chemical composition during alloy development by evaluating tensile properties with minimum volume of material.

  7. Evolving radio structure of the binary star SS433 at a resolution of 15 marc s

    International Nuclear Information System (INIS)

    A comprehensive series of VLBI (very-long-baseline interferometry) observations of SS433, carried out over an 11-day period on the European VLBI Network at 5 GHz, reveals the motion and evolution of a series of faint 'blobs' ejected from the system. These blobs are observed to brighten at a substantial distance from the binary star. (author)

  8. Optimal continuous order quantity (s,S) policies; the 45-degrees algorithm

    NARCIS (Netherlands)

    E.M. Bazsa-Oldenkamp; P. den Iseger

    2002-01-01

    textabstractThe most recent optimization algorithm for (s,S) order policies with continuous demand was developed by Federgruen and Zipkin (1985). This was also the first efficient algorithm, which uses policy iteration instead of discretization. Zheng and Federgruen (1991) developed an even more eff

  9. Possibility of Inducing Compressive Residual Stresses in Welded Joints of SS400 Steels

    Institute of Scientific and Technical Information of China (English)

    2001-01-01

    Since the welded constructions produce easily stress corrosion cracking (SCC) or fatigue disruption in corrosive medium or under ripple load, two methods inducing compressive stress on structural surfaces by anti-welding-heating treatment (AWHT) and explosion treatment (ET) are presented. The results show that they are good ways to resisting SCC on the welded SS400 steel or other components.

  10. Intermittency in 32S+S and 32S+Au collisions at the CERN SPS

    International Nuclear Information System (INIS)

    Nonstatistical or 'intermittent' fluctuations of charged particle multiplicities have been investigated at the CERN SPS with the WA80 multiplicity array for 32S+S and 32S+Au collisions of varying centrality. Within the phase space domain studied there is no evidence for intermittency in these collisions beyond that accounted for by FRITIOF filtered through a full detector simulation. (orig.)

  11. Is PiSS Alpha-1 Antitrypsin Deficiency Associated with Disease?

    Directory of Open Access Journals (Sweden)

    Dawn McGee

    2010-01-01

    Full Text Available Background. Alpha-1 antitrypsin deficiency (AAT is an inherited condition that predisposes to lung and/or liver disease. Objective. The current study examined the clinical features of the PiSS genotype. Methods. Nineteen study participants (PiSS and 29 matched control participants (PiMM were telephone interviewed using a standardized questionnaire. Demographic features, cigarette smoking, vocation, medication history, and clinical diagnoses were compared. Statistical analysis was performed. Finally, a comprehensive literature review was performed by two investigators. Results. 12/19 (63.2% study participants reported the presence of lung and/or liver disease compared to 12/29 (41.4% control participants. There trended toward having a higher frequency of medication allergies in the study population (42.11% versus 20.69%. Conclusions. The PiSS genotype was associated with a similar incidence of obstructive lung disease to controls. Selective bias intrinsic in testing for AAT deficiency and the rarity of the PiSS genotype will make future study of this association dependent on population-based tests.

  12. On the Optimality of (s,S) Inventory Policies: A Quasivariational Approach

    OpenAIRE

    Lakdere Benkherouf

    2008-01-01

    This paper revisits the classical discrete-time stationary inventory model. A new proof, based on the theory of quasivariational inequality (QVI), of the optimality of (s,S) policy is presented. This proof reveals a number of interesting properties of the optimal cost function. Further, the proof could be used as a tutorial for applications of QVI to inventory control.

  13. PU/SS Eutectic Assessment In 9975 Packagings In A Storage Facility During Extended Fire

    International Nuclear Information System (INIS)

    In a radioactive material (RAM) packaging, the formation of eutectic at the Pu/SS (plutonium/stainless steel) interface is a serious concern and must be avoided to prevent of leakage of fissile material to the environment. The eutectic temperature for the Pu/SS is rather low (410 C) and could seriously impact the structural integrity of the containment vessel under accident conditions involving fire. The 9975 packaging is used for long term storage of Pu bearing materials in the DOE complex where the Pu comes in contact with the stainless steel containment vessel. Due to the serious consequences of the containment breach at the eutectic site, the Pu/SS interface temperature is kept well below the eutectic formation temperature of 410 C. This paper discusses the thermal models and the results for the extended fire conditions (1500 F for 86 minutes) that exist in a long term storage facility and concludes that the 9975 packaging Pu/SS interface temperature is well below the eutectic temperature.

  14. Neutral strange particle production in S-S collisions at 200 GeV/nucleon

    International Nuclear Information System (INIS)

    In this paper data on strange particle production in S-S collisions at 200 GeV/n are presented and analyzed in order to study the volume dependence of the strangeness production. We report on the yield of Λ and Ks0 as a function of collision centrality. (orig./HSI)

  15. Summary of Tests to Determine Effectiveness of Gelatin Strike on SS{ampersand}C Dissolver Solutions

    Energy Technology Data Exchange (ETDEWEB)

    Murray, A.M. [Westinghouse Savannah River Company, AIKEN, SC (United States); Karraker, D.G.

    1998-05-01

    The solutions from the dissolution of sand, slag, and crucible (SS&C) material are sufficiently different from previous solutions processed via the F-Canyon Purex process that the effectiveness of individual process steps needed to be ascertained. In this study, the effectiveness of gelatin strike was tested under a variety of conditions. Specifically, several concentrations of silica, fluoride, nitric acid (HNO{sub 3}), boric acid (H{sub 3}BO{sub 3}), and aluminium nitrate nonahydrate (ANN) were studied. The disengagement times of surrogate and plant SS&C dissolver solutions from plant solvent also were measured. The results of the tests indicate that gelatin strike does not coagulate the silica at the low concentration of silica ({tilde 30} ppm) expected in the SS&C dissolver solutions because the silicon is complexed with fluoride ions (e.g., SiF{sub 6}{sup -2}). The silicon fluoride complex is expected to remain with the aqueous phase during solvent extraction. The disengagement times of the dissolver solutions from the plant solvent were not affected by the presence of low concentrations of silica and no third phase formation was observed in the disengagement phase with the low silica concentrations. Tests of surrogate SS&C dissolver solutions with higher concentration of silica (less than 150 ppm) did show that gelatin strike followed by centrifugation resulted in good phase disengagement of the surrogate SS{ampersand}C dissolver solution from the plant dissolver solution. At the higher silica concentrations, there is not sufficient fluoride to complex with the silica, and the silica must be entrained by the gelatin and removed from the dissolver solution prior to solvent extraction.

  16. First insights into the genetic diversity of Echinococcus granulosus sensu stricto (s.s.) in Serbia.

    Science.gov (United States)

    Debeljak, Zoran; Boufana, Belgees; Interisano, Maria; Vidanovic, Dejan; Kulisic, Zoran; Casulli, Adriano

    2016-06-15

    Cystic echinococcosis (CE) caused by Echinococcus granulosus sensu stricto (s.s.) is a cosmopolitan zoonotic infection which is endemic in Serbia where it is subject to mandatory reporting. However, information on the incidence of the disease in humans and prevalence of hydatid infection in livestock remains limited. We used sequenced data of the cytochrome c oxidase subunit 1 (cox 1) mitochondrial gene to examine the genetic diversity and population structure of E. granulosus (s.s.) from intermediate hosts from Serbia. We also compared our generated nucleotide sequences with those reported for neighbouring European countries. Echinococcus canadensis was molecularly confirmed from pig and human hydatid isolates. E. granulosus (G1) was confirmed from sheep and cattle hydatid isolates as well as the first molecular confirmation in Serbia of E. granulosus G2 in sheep and E. granulosus G3 in sheep and cattle hydatid isolates. The Serbian E. granulosus (s.s.) parsimony network displayed 2 main haplotypes (SB02 and SB05) which together with the neutrality indices were suggestive of bottleneck and/or balancing selection. Haplotype analysis showed the presence of the common E. granulosus haplotype described from other worldwide regions. Investigation of the pairwise fixation (Fst) index suggested that Serbian populations of E. granulosus (s.s.) from sheep and cattle hosts showed moderate genetic differentiation. Six of the Serbian haplotypes (SB02-SB07) were shared with haplotypes from Bulgaria, Hungary and/or Romania. Further studies using a larger number of hydatid isolates from various locations across Serbia will provide more information on the genetic structure of E. granulosus (s.s.) within this region. PMID:27198778

  17. Fatigue Crack Propagation from Notched Specimens of 304 SS in elevated Temperature Aqueous Environment

    Energy Technology Data Exchange (ETDEWEB)

    Wire, G. L.; Mills, W. J.

    2002-08-01

    Fatigue crack propagation (FCP) rates for 304 stainless steel (304SS) were determined in 24 degree C and 288 degree C air and 288 degree C water using double-edged notch (DEN) specimens of 304 stainless steel (304 SS). Test performed at matched loading conditions in air and water at 288 degree C with 20-6- cc h[sub]2/kg h[sub]2O provided a direct comparison of the relative crack growth rates in air and water over a wide range of crack growth rates. The DEN crack extension ranged from short cracks (0.03-0.25 mm) to long cracks up to 4.06 mm, which are consistent with conventional deep crack tests. Crack growth rates of 304 SS in water were about 12 times the air rate. This 12X environmental enhancement persisted to crack extensions up to 4.06 mm, far outside the range associated with short crack effects. The large environmental degradation for 304 SS crack growth is consistent with the strong reduction of fatigue life in high hydrogen water. Further, very similar environmental effects w ere reported in fatigue crack growth tests in hydrogen water chemistry (HWC). Most literature data in high hydrogen water show only a mild environmental effect for 304 SS, of order 2.5 times air or less, but the tests were predominantly performed at high cyclic stress intensity or equivalently, high air rates. The environmental effect in low oxygen environments at low stress intensity depends strongly on both the stress ratio, R, and the load rise time, T[sub]r, as recently reported for austenitic stainless steel in BWR water. Fractography was performed for both tests in air and water. At 288 degree C in water, the fracture surfaces were crisply faceted with a crystallographic appearance, and showed striations under high magnification. The cleavage-like facets on the fracture surfaces suggest that hydrogen embrittlement is the primary cause of accelerated cracking.

  18. Escherichia coli RecO protein anneals ssDNA complexed with its cognate ssDNA-binding protein: A common step in genetic recombination

    OpenAIRE

    Kantake, Noriko; Madiraju, Murty V. V. M.; Sugiyama, Tomohiko; Kowalczykowski, Stephen C.

    2002-01-01

    We present biochemical evidence for the functional similarity of Escherichia coli RecO protein and bacteriophage T4 UvsY protein to eukaryotic Rad52 protein. Although Rad52 protein is conserved in eukaryotes, no sequence homologue has been found in prokaryotes or archeabacteria. Rad52 protein has two unique activities: facilitation of replication protein-A (RPA) displacement by Rad51 protein and annealing of RPA–single-stranded DNA (ssDNA) complexes. Both activities require species-specific i...

  19. Improvisointi kanttorin työssä : "...lupa olla välittämässä sitä virtaa, joka on olemassa."

    OpenAIRE

    Perko, Johannes

    2011-01-01

    Tämän opinnäytetyön tavoitteena oli kartoittaa improvisoinnin käytettävyyttä ja käyttökokemuksia kanttorin työssä. Aluksi luodaan yleiskuva kanttorin viran historiaan ja improvisoinnin opetukseen keskeisissä kirkkomusiikkikouluissa. Improvisaation käsitettä avataan yksittäisten muusikoiden kokemuksilla. Muusikoita oli haastateltu RONDO-lehden artikkeleissa. Kanttorien improvisointikäytäntöjä on tutkittu vähän. Siksi tämä tutkielma sisältää kyselyn, joka lähetettiin Turun arkkihiippakunnan...

  20. "Lähtökohtana kohdata ihminen ihmisenä" - : käsityksiä monikulttuurisesta osaamisesta etsivässä nuorisotyössä Helsingissä

    OpenAIRE

    Pulli, Jatta

    2013-01-01

    ssä opinnäytetyössä tarkastellaan käsityksiä monikulttuurisesta osaamisesta etsivässä nuorisotyössä Helsingissä. Monikulttuurisesta nuorisotyöstä on olemassa useita tutkimuksia ja samaten etsivästä nuorisotyöstä on kirjoitettu ja siitä ollaan tietoisia, varsinkin etsivän nuorisotyön Nuorisolakiin lisäyksen myötä 2011. Näitä kahta yhdessä ei kuitenkaan ole tarkasteltu paljoakaan. Tavoitteena opinnäytetyössä on saada tietää käytännön etsivää nuorisotyötä tekeviltä tahoilta, miten he miel...

  1. Cyclic lipopeptide surfactant production by Pseudomonas fluorescens SS101 is not required for suppression of complex Pythium spp. populations

    Science.gov (United States)

    Previously, zoosporicidal activity and control of Pythium intermedium by Pseudomonas fluorescens strain SS101 was attributed, in part, to the production of the cyclic lipopeptide surfactant massetolide A. In the current study, capacity of SS101 and its surfactant-deficient mutant strain 10.24 to sup...

  2. MADS-Box Transcription Factor SsMADS Is Involved in Regulating Growth and Virulence in Sclerotinia sclerotiorum

    Directory of Open Access Journals (Sweden)

    Xiaoyan Qu

    2014-05-01

    Full Text Available MADS-box proteins, a well-conserved family of transcription factors in eukaryotic organisms, specifically regulate a wide range of cellular functions, including primary metabolism, cell cycle, and cell identity. However, little is known about roles of the MADS-box protein family in the fungal pathogen Sclerotinia sclerotiorum. In this research, the S. sclerotiorum MADS-box gene SsMADS was cloned; it encodes a protein that is highly similar to Mcm1 orthologs from Saccharomyces cerevisiae and other fungi, and includes a highly conserved DNA-binding domain. MADS is a member of the MADS box protein SRF (serum response factor lineage. SsMADS function was investigated using RNA interference. Silenced strains were obtained using genetic transformation of the RNA interference vectors pS1-SsMADS and pSD-SsMADS. SsMADS expression levels in silenced strains were analyzed using RT-PCR. The results showed that SsMADS mRNA expression in these silenced strains was reduced to different degrees, and growth rate in these silenced strains was significantly decreased. Infecting tomato leaflets with silenced strains indicated that SsMADS was required for leaf pathogenesis in a susceptible host. Our results suggest that the MADS-box transcription factor SsMADS is involved in S. sclerotiorum growth and virulence.

  3. Self-assembly of ssDNA-amphiphiles into micelles, nanotapes and nanotubes

    Science.gov (United States)

    Pearce, Timothy R.

    The field of DNA nanotechnology utilizes DNA as a construction material to create functional supramolecular and multi-dimensional structures like two-dimensional periodic lattices and three-dimensional polyhedrons with order on the nanometer scale for many nanotechnology applications including molecular templating, nanosensors, and drug delivery. Single-stranded DNA (ssDNA) is often used to create these nanostructures as the DNA bases provide an intrinsic molecular code that can be exploited to allow for the programmed assembly of structures based upon Watson-Crick base-pairing. However, engineering these complex structures from biopolymers alone requires careful design to ensure that the intrinsic forces responsible for organizing the materials can produce the desired structures. Additional control over supramolecular assembly can be achieved by chemically modifying the ssDNA with hydrophobic moieties to create amphiphilic molecules, which adds the hydrophobic interaction to the list of contributing forces that drive the self-assembly process. We first explored the self-assembly behavior of a set of ssDNA aptamer-amphiphiles composed of the same hydrophobic tail and hydrophilic ssDNA aptamer headgroup but with different spacer molecules linking these groups together. Through the use of cryo-transmission electron microscopy (cryo-TEM), small angle x-ray scattering (SAXS), and circular dichroism (CD) we show that the aptamer-amphiphiles can assemble into a variety of structures depending on the spacer used. We demonstrated, for the first time, the creation of self-assembled aptamer-amphiphile nanotape structures and show that the choice of the spacer used in the design of aptamer-amphiphiles can influence their supramolecular self-assembly as well as the secondary structure of the aptamer headgroup. We next explored the role of the ssDNA headgroup on the amphiphile self-assembly behavior by designing amphiphiles with headgroups of multiple lengths and nucleotides

  4. Salassapidon haasteet työelämässä

    OpenAIRE

    Puolatie, Miia

    2013-01-01

    Sosiaalisen median vaikutus kasvaa vuosi vuodelta enemmän. Salassapidon haasteet kasvavat näiden sosiaalisten medioiden vaikutusten myötä. Työsopimuslain mukaan työnantajan liike – ja ammattisalaisuuksia ei saa kertoa eteenpäin, eikä saa yrittää tahallisesti hyötyä näistä tiedoista. Laki ei kuitenkaan kerro mitä tällaiset liike – ja ammattisalaisuudet ovat. Tässä työssä tutustuttiin salassapidon haasteisiin verraten työsopimuslakia työntekijöiden käytännön tietoihin. Samalla myös tutkittii...

  5. Uncertainty Estimation of Analysis of Hg in Fish and Shrimp Sample by SS-AAS Method

    International Nuclear Information System (INIS)

    An uncertainty estimation of analysis of Hg in Fish and Shrimp sample by SS-AAS method has been done. Samples preparation have been done by using freeze drying. They were then homogenized and sieved, so their grain size of 100 mesh were obtained. The content of Hg in the samples and SRM 1566a has been analyzed by using SS-AAS (solid sampling - atomic absorption spectrometer) AAS 5 EA, ZEISS. The experimental result shows that expanded uncertainty value of fish sample is 4.3636, whereas expanded uncertainty value of shrimp’s sample = 2.638. Precision value is = 98.46 % and accuracy value = 97.0 % and limit of detection = 0.0154 ng/g. (author)

  6. Valtion metsien kehitys Kittilässä vuodesta 1924 vuoteen 2011

    OpenAIRE

    Vaara, Ilkka

    2012-01-01

    Metsähallituksen ensimmäisiä tehtäviä perustamisen jälkeen oli hankkia tiedot maa- ja metsäomaisuudesta. Vuosisadan vaihteen jälkeen arviointeja alettiin tehdä 10 – 20 vuoden välein. Paikkatietotietojärjestelmien kehittymi-sen myötä jatkuvaan ylläpitoon siirryttiin 1990-luvulla Tässä opinnäytetyössä pääasiallisena tietolähteenä ovat Metsähallituksen Kittilän hoitoalueen metsätaloussuunnitelmat sekä nykyiset maankäyttö- ja kuviotiedot. Koko Suomea koskeva tarkastelu perustuu pääasiassa Me...

  7. Instagramin suunnitelmallinen käyttö kaupunkien sosiaalisen median viestinnässä

    OpenAIRE

    Pihlajamäki, Laura

    2015-01-01

    Tämän opinnäytetyön tarkoituksena oli selvittää, mitkä eri tekijät ohjaavat Instagramin käyttöä kaupunkien sosiaalisen median viestinnässä. Opinnäytetyön toimeksiantaja oli Riihimäen kaupunki, jonka tavoitteena on perustaa oma Instagram-tili lähitulevaisuudessa jo käytössä olevien sosiaalisen media palveluiden lisäksi. Ennen oman Instagram-tilin luomista Riihimäen kaupunki halusi saada vinkkejä palvelun suunnitelmalliseen käyttöön. Työn teoreettinen viitekehys koostuu pääosin sosiaalista ...

  8. Antibody (MAb) disulfide (-S-S-) groups: Controlled reduction, quantification and influence on immunoreactivity

    International Nuclear Information System (INIS)

    Disulfide groups reduced to sulfhydryls may provide high affinity Tc-99m binding. Since each ug of IgM has 1.1 x 1014 -S-S- bridges, IgG or F(ab')2 have 1.4 x 1014, and 20mCi Tc-99m contains 2.3 x 1013 atoms, 100 ug of any above MAb could be labeled with 20mCi Tc-99m, with <1% of it's -S-S- reduced. Agents SnCl2, 2-mercaptoethanol, Dithiothreitol, Dithioerythritol, and ascorbic acid were investigated and protein to agent molar ratios determined (1:500, 1:1,000, 1:1,000, 1:1,000 and 1:3,500 respectively) for Tc-99m binding which was 65±3%, 55±3% and 98±5% respectively

  9. Wear and Corrosion Study of Sputtered Zirconium thin films on SS316L for Windmill Application

    Directory of Open Access Journals (Sweden)

    Arunkumar N

    2015-05-01

    Full Text Available The Aim of this study is to observe the Wear and Corrosion behavior of Zirconium coated 316L stainless steel. After polishing, SS316L was coated with Zirconium employing DC sputtering process (a technique of physical vapor deposition.Structure characterization techniques including Scanning Electron Microscope (SEM and X-Ray Diffraction (XRD were utilized to investigate the microstructure and crystallinity of the coating. Salt spray test was performed by spraying Sodium chloride in order to determine corrosion resistance behavior of the coated sample. Pin on disc wear test was performed by hardened and tempered EN31 steel pin in order to determine and compare the Wear resistance behavior of Coated and uncoated samples. The Objective is to recommend the zirconium coated Stainless steel SS316L can be a choice for Off-shore wind mills where the shafts undergo Wear and corrosion problems.

  10. 3D hydrodynamical simulations of the large scale structure of W50-SS433

    CERN Document Server

    Zavala, Jesús; Cerqueira, Adriano H; Dubner, Gloria M

    2008-01-01

    We present 3D hydrodynamical simulations of a precessing jet propagating inside a supernova remnant (SNR) shell, particularly applied to the W50-SS433 system in a search for the origin of its peculiar elongated morphology. Several runs were carried out with different values for the mass loss rate of the jet, the initial radius of the SNR, and the opening angle of the precession cone. We found that our models successfully reproduce the scale and morphology of W50 when the opening angle of the jets is set to 10$\\degr$ or if this angle linearly varies with time. For these models, more realistic runs were made considering that the remnant is expanding into an interstellar medium (ISM) with an exponential density profile (as HI observations suggest). Taking into account all these ingredients, the large scale morphology of the W50-SS 433 system, including the asymmetry between the lobes (formed by the jet-SNR interaction), is well reproduced.

  11. Bioconversion of glycerol for bioethanol production using isolated Escherichia coli SS1

    Directory of Open Access Journals (Sweden)

    Sheril Norliana Suhaimi

    2012-06-01

    Full Text Available Bioconverting glycerol into various valuable products is one of glycerol's promising applications due to its high availability at low cost and the existence of many glycerol-utilizing microorganisms. Bioethanol and biohydrogen, which are types of renewable fuels, are two examples of bioconverted products. The objectives of this study were to evaluate ethanol production from different media by local microorganism isolates and compare the ethanol fermentation profile of the selected strains to use of glucose or glycerol as sole carbon sources. The ethanol fermentations by six isolates were evaluated after a preliminary screening process. Strain named SS1 produced the highest ethanol yield of 1.0 mol: 1.0 mol glycerol and was identified as Escherichia coli SS1 Also, this isolated strain showed a higher affinity to glycerol than glucose for bioethanol production.

  12. The algebra of observables in Gau{\\ss}ian normal spacetime coordinates

    CERN Document Server

    Bodendorfer, Norbert; Lewandowski, Jerzy; Świeżewski, Jędrzej

    2016-01-01

    We discuss the canonical structure of a spacetime version of the radial gauge, i.e. Gau{\\ss}ian normal spacetime coordinates. While it was found for the spatial version of the radial gauge that a "local" algebra of observables can be constructed, it turns out that this is not possible for the spacetime version. The technical reason for this observation is that the new gauge condition needed to upgrade the spatial to a spacetime radial gauge does not Poisson-commute with the previous gauge conditions. It follows that the involved Dirac bracket is inherently non-local in the sense that no complete set of observables can be found which is constructed locally and at the same time has local Dirac brackets. A locally constructed observable here is defined as a finite polynomial of the canonical variables at a given physical point specified by the Gau{\\ss}ian normal spacetime coordinates.

  13. Teatterisavumerkkejä - Kommunikointi työryhmässä

    OpenAIRE

    Nenonen, Camilla

    2011-01-01

    BA-opinnäytetyössä Teatterisavumerkkejä – Kommunikointi työryhmässä tarkasteltiin suunnittelijatyöryhmän kommunikointia teatterilavastajan näkökulmasta. Havainnot olivat pääosin tekijän taiteellisen opinnäytteen YOU’RE MY HIT AND YOU’RE MY SHIT tekoprosessista. Lisäksi viitattiin muihin produktioihin, joissa tekijä oli ollut mukana, sekä teatterin ammattilaisten näkemyksiin aiheesta. Opinnäytteen tavoitteena oli oppia enemmän lavastajan roolista taiteellisen työryhmän jäsenenä ja sen myötä ke...

  14. Siivoustyön kehittäminen Campusravita Oy:ssä : Esivalmisteltu siivous

    OpenAIRE

    Matilainen, Mari

    2014-01-01

    Työn tavoitteena oli tehdä Campusravita Oy:lle uusi siivoussuunnitelma. Tavoitteena oli tehdä suunnitelma joka mukailee esivalmistellun siivouksen periaatteita. Tässä työssä huomioitiin kestävä kehitys jätteen ja veden osalta. Näiden lisäksi haluttiin osoittaa syntyneet säästöt ajallisesti ja taloudellisesti. Laskelmilla pyrittiin näyttämään kuinka paljon kuluu rahaa eri siivousmenetelmillä ja vertailla niitä keskenään. Työn tavoitteena oli löytää mahdollisimman toimiva ratkaisu Campusr...

  15. Työaika-autonomia ja Mansikkapaikan palvelukotien hoitajien työssä jaksaminen

    OpenAIRE

    Kokkomäki, Saana

    2015-01-01

    Opinnäytetyön tarkoituksena oli kehittää edelleen Rauman Mansikkapaikan palvelukotien hoitajien käytössä olevaa työaika-autonomista työvuorosuunnittelua. Tavoitteena oli saada tietoa työaika-autonomisen työvuorosuunnittelun hyvistä käytännöistä sekä tunnistaa mahdolliset kehittämistarpeet. Tavoitteena oli myös kuvata Mansikkapaikan palvelukotien hoitajien kokemuksia työaika-autonomian merkityksestä työssä jaksamiseen. Opinnäytetyön tutkimusmenetelmänä käytettiin Delfoi-menetelmää. Ainei...

  16. EVALUATION OF MECHANICAL PROPERTIES OF SS 316 L WELDMENTS USING TUNGSTEN INERT GAS WELDING

    Directory of Open Access Journals (Sweden)

    S.V. ABINESH KUMAR

    2012-05-01

    Full Text Available This work aims at joining of similar grades of stainless steel by TIG welding with the various parameters like current, bevel angle and gas flow rate. The SS 316L is selected over other grades due to its lesser carbon content it is used in pressure vessels for corrosive liquids etc. The rod of SS 316L of 25 mm diameter and 75 mm lengthwas used as the base material for this experiment. The rod was machined in accordance to ASTM-A-370 standards for finding the echanical behaviour like Tensile strength, micro hardness and micro structure. Higher tensile strength achieved with a current of 110A, bevel angle of 600 and a gas flow rate of 0.7 LPM. Non-destructive tests like radiographic tests were performed to find the defects in the joints. The defect incurred was lack of penetration and it was observed in the sample D and sample G.

  17. Crack propagation tests of HIPed DSCu/SS joints for plasma facing components

    International Nuclear Information System (INIS)

    Aluminum oxide dispersion strengthened copper (DSCu), used as the heat sink, and AISI 316L(N) type stainless steel (SS), used for cooling pipes, were metallurgically joined to be used as first wall of plasma facing components. Hot isostatic pressing (HIP) was proposed as the joining fabrication technique in the first-wall/blanket components. In this study, fracture toughness and fatigue crack growth rate tests were carried out for the fracture strength evaluation of HIPed joints. Permissible crack lengths during fabrication of first-wall components were evaluated from the results of the fracture toughness tests. In crack growth rate tests, the crack prepared in the DSCu propagated, turned at the HIPed interface, and propagated along it. Therefore, it was found that the crack provided in the DSCu heat sink did not propagate through the SS cooling pipe

  18. An accurate geometric distance to the compact binary SS Cygni vindicates accretion disc theory

    CERN Document Server

    Miller-Jones, J C A; Knigge, C; Körding, E G; Templeton, M; Waagen, E O

    2013-01-01

    Dwarf novae are white dwarfs accreting matter from a nearby red dwarf companion. Their regular outbursts are explained by a thermal-viscous instability in the accretion disc, described by the disc instability model that has since been successfully extended to other accreting systems. However, the prototypical dwarf nova, SS Cygni, presents a major challenge to our understanding of accretion disc theory. At the distance of 159 +/- 12 pc measured by the Hubble Space Telescope, it is too luminous to be undergoing the observed regular outbursts. Using very long baseline interferometric radio observations, we report an accurate, model-independent distance to SS Cygni that places the source significantly closer at 114 +/- 2 pc. This reconciles the source behavior with our understanding of accretion disc theory in accreting compact objects.

  19. CLaSS Computer Literacy Software: From Design to Implementation - A Three Year Student Evaluation

    Directory of Open Access Journals (Sweden)

    Ian Cole

    2006-12-01

    Full Text Available Both computer literacy and information retrieval techniques are required to undertake studies in higher education in the United Kingdom. This paper considers the research, development and the 3-year student evaluation of a piece of learning technology in computer and information literacy (CLaSS software. Students completed a questionnaire to examine their own assessment of knowledge and competence in computer and information literacy and based on this assessment CLaSS software was created to assist nursing students with computer and information literacy. This paper draws on existing literature and applies a specific learning model to the software while considering software engineering and user-centered design methodologies. The technical processes involved in designing and creating the software are briefly considered with software development data analysis discussed. A 3-year student evaluation of the software after it's release was undertaken to consider the long-term validity and usefulness of this software with the results analysed and discussed.

  20. EBSD analysis of magnesium addition on inclusion formation in SS400 structural steel

    Energy Technology Data Exchange (ETDEWEB)

    Luo, Sin-Jie [Department of Materials Science and Engineering, National Cheng-Kung University, No. 1, University Road, Tainan 701, Taiwan (China); Su, Yen-Hao Frank; Lu, Muh-Jung [China Steel Corporation, Kaohsiung 81233, Taiwan (China); Kuo, Jui-Chao, E-mail: jckuo@mail.ncku.edu.tw [Department of Materials Science and Engineering, National Cheng-Kung University, No. 1, University Road, Tainan 701, Taiwan (China)

    2013-08-15

    In this study, the effect of magnesium addition on the inclusion formation in SS400 steel was investigated. The experimental specimens with and without Mg addition treatment were compared. The microstructure was observed using optical microscopy after etching with 3% nital. The morphology and chemical composition of the inclusions were analyzed via scanning electron microscopy and energy dispersive spectrometry. The lattice structure and orientation of the inclusions were identified by electron backscattering diffraction. The average size of inclusions in SS400 was between 0.67 and 0.75 μm, and between 0.65 and 0.68 μm in SS400 + Mg. The 2 ppm Mg addition resulted in the oxide formation change from Al{sub 2}O{sub 3} to MgO·Al{sub 2}O{sub 3} and in the inclusion formation change from Al{sub 2}O{sub 3}–MnS to MgO·Al{sub 2}O{sub 3}–MnS. Moreover, a simple-phase MnS with an average grain size of 1 μm to 2 μm was observed in rod-like, globular, and polyhedron forms. - Highlights: • The effect of magnesium addition was investigated for SS400 steel. • 2 ppm Mg addition changes the inclusion formation from Al2O3-MnS to MgO·Al2O3-MnS. • MnS observed in inclusions exhibits rod-like, globular, and polyhedron forms.

  1. Upper Mantle Discontinuity Structure Beneath the Western Atlantic Ocean and Eastern North America from SS Precursors

    Science.gov (United States)

    Schmerr, N. C.; Beghein, C.; Kostic, D.; Baldridge, A. M.; West, J. D.; Nittler, L. R.; Bull, A. L.; Montesi, L.; Byrne, P. K.; Hummer, D. R.; Plescia, J. B.; Elkins-Tanton, L. T.; Lekic, V.; Schmidt, B. E.; Elkins, L. J.; Cooper, C. M.; ten Kate, I. L.; Van Hinsbergen, D. J. J.; Parai, R.; Glass, J. B.; Ni, J.; Fuji, N.; McCubbin, F. M.; Michalski, J. R.; Zhao, C.; Arevalo, R. D., Jr.; Koelemeijer, P.; Courtier, A. M.; Dalton, H.; Waszek, L.; Bahamonde, J.; Schmerr, B.; Gilpin, N.; Rosenshein, E.; Mach, K.; Ostrach, L. R.; Caracas, R.; Craddock, R. A.; Moore-Driskell, M. M.; Du Frane, W. L.; Kellogg, L. H.

    2015-12-01

    Seismic discontinuities within the mantle arise from a wide range of mechanisms, including changes in mineralogy, major element composition, melt content, volatile abundance, anisotropy, or a combination of the above. In particular, the depth and sharpness of upper mantle discontinuities at 410 and 660 km depth are attributed to solid-state phase changes sensitive to both mantle temperature and composition, where regions of thermal heterogeneity produce topography and chemical heterogeneity changes the impedance contrast across the discontinuity. Seismic mapping of this topography and sharpness thus provides constraint on the thermal and compositional state of the mantle. The EarthScope USArray is providing unprecedented access to a wide variety of new regions previously undersampled by the SS precursors. This includes the boundary between the oceanic plate in the western Atlantic Ocean and continental margin of eastern North America. Here we use a seismic array approach to image the depth, sharpness, and topography of the upper mantle discontinuities, as well as other possible upper mantle reflectors beneath this region. This array approach utilizes seismic waves that reflect off the underside of a mantle discontinuity and arrive several hundred seconds prior to the SS seismic phase as precursory energy. In this study, we collected high-quality broadband data SS precursors data from shallow focus (4th root vespagrams to enhance the SS precursors and determine how they sample the mantle. Our data show detection of localized structure on the discontinuity boundaries as well as additional horizons, such as the X-discontinuity and a potential reflection from a discontinuity near the depth of the lithosphere-asthenosphere boundary. These structures are related to the transition from predominantly old ocean lithosphere to underlying continental lithosphere, as while deeper reflectors are associated with the subduction of the ancient Farallon slab. A comparison of the

  2. Das Kindersprachscreening (KiSS) - ein Verfahren zur Sprachstandserfassung vierjähriger Kinder

    OpenAIRE

    NEUMANN, K; Holler-Zittlau, I; Sick, U; Zaretsky, Y; Euler, HA

    2009-01-01

    Einleitung: Zur Feststellung von Sprachförder- oder -therapiebedarf eignet sich ein Sprachscreening, sofern es eine ausreichende Validität aufweist und über streng algorithmische Kriterien zur Auffälligkeitsklassifizierung verfügt. Material und Methode: Für eine hessenweite Erfassung der vorschulischen Sprachkompetenz wurde das psychometrisch geprüfte Kindersprachscreening (KiSS) entwickelt. 257 Kinder im Alter von 4;0 bis 4;5 Jahren wurden mit einer Testbatterie auf ihren Sprachstand ...

  3. Liiketoiminnan jatkuvuus IT – ympäristössä : Case: BASF

    OpenAIRE

    Orpana, Mari

    2010-01-01

    Yritykset ovat varautuneet puutteellisesti tietotekniikkaan liittyviin onnettomuuksiin, vaikka yritysten riippuvuus tietoteknisien järjestelmien käytettävyydestä on jatkuvasti kasvanut. Vakavien häiriötilanteiden sattuessa yritys on suurissa vaikeuksissa, jos liiketoiminnan jatkuvuussuunnitelmaa ei ole tehty. Vuonna 2008 BASF konsernin Pohjoismaiden suurimaassa tuotantolaitoksessa sattui kriittisessä prosessissa useamman päivän käyttökatkos IT – ympäristössä tapahtuneen virheen vuoksi. Tä...

  4. Hei, mä osaan! : lasten visuomotoristen taitojen kehittymisen tukeminen toimintaterapiaryhmässä

    OpenAIRE

    Hokkanen, Teija; Riekki, Kristiina

    2006-01-01

    Tutkimustehtävänä oli selvittää tukiko Palokan terveysasemalla toteutettu toimintaterapiaryhmä lasten visuomotoristen taitojen kehitystä. Tutkimuksen tavoitteena oli myös selvittää seikkailun käytön mahdollisuuksia toimintaterapiaryhmässä. Tutkimus toteutettiin osana Palokan terveydenhuollon toimintaterapiapalveluja. Tutkimuksessa ohjattiin kuuden esikouluikäisen lapsen toimintaterapiaryhmää, jonka tavoitteena oli tukea visuomotoristen taitojen kehitystä kouluvalmiuksia ajatellen. Visuomotori...

  5. Lapsen osallistumisen tarkastelu COSA 2.2 -menetelmässä

    OpenAIRE

    Korpelainen, Petra; Levomäki, Taru; Sell, Anna

    2016-01-01

    Lasten osallistuminen kuntoutuksessa ei toteudu Suomessa riittävissä määrin. Opinnäytetyössä selvitettiin vastausta siihen, millaisia tekijöitä Lasten toimintamahdollisuuksien itsearvioinnissa (Child Occupational Self Assessment, COSA) on lapsen osallistumisen mahdollistamiseksi. Opinnäytetyön yhteistyökumppanina toimi Metropolia Ammattikorkeakoulun toimintaterapian tutkinto-ohjelma. Opinnäytetyön tavoitteena oli selvittää, miten COSA-itsearvioinnin avulla voidaan mahdollistaa lasten osallist...

  6. Mass loss by SS 433, and its effect on the x-ray and radio emission

    International Nuclear Information System (INIS)

    Analysis of the hydrogen emission lines and infrared radiation of SS 433 indicates that this binary system (comprising a massive giant and a neutron star) is surrounded by an envelope of effective size roughly-equal1013 cm and mean density rhoroughly-equal10-13 g/cm3. Since random plasma velocities of roughly-equal(2--3) x 108 cm/sec occur in this envelope, gas is continually escaping from the system at a rate of roughly-equal10-4 M/sub sun//yr. Gas would be replenished in the envelope on a time scale of hours. This mass loss by the SS 433 system cannot be attributed solely to stellar wind from the massive component. Gas evidently is streaming rapidly through the inner Lagrangian point. Some mechanism should operate to expel from the system the gas being exchanged by components. One possibility is a propeller effect due to a swift spin (Ωroughly-equal103 sec-1) of the neutron star, whose magnetic field should not be too strong. The observed x rays do not come from gas being accreted onto the compact object; instead, they are emitted when the clouds being ejected from the system interact with the ambient medium. The distinctive aspherical shape of the associated supernova remnant W50 reflects the regular expulsion of plasma clouds from SS 433 within a narrow solid angle. These clouds would have a combined energy Eroughly-equal3 x 1050 erg, in good accord with the observed rate at which the source is releasing kinetic energy (Wroughly-equal3 x 1038 erg/sec). The radio variability of SS 433 is discussed; it results from the rapid motion of the clouds through the surrounding radio-opaque plasma

  7. High-frequency genome editing using ssDNA oligonucleotides with zinc-finger nucleases

    DEFF Research Database (Denmark)

    Chen, Fuqiang; Pruett-Miller, Shondra M; Huang, Yuping; Gjoka, Monika; Duda, Katarzyna; Taunton, Jack; Collingwood, Trevor N; Frödin, Morten; Davis, Gregory D

    2011-01-01

    Zinc-finger nucleases (ZFNs) have enabled highly efficient gene targeting in multiple cell types and organisms. Here we describe methods for using simple ssDNA oligonucleotides in tandem with ZFNs to efficiently produce human cell lines with three distinct genetic outcomes: (i) targeted point mut...... mutation, (ii) targeted genomic deletion of up to 100 kb and (iii) targeted insertion of small genetic elements concomitant with large genomic deletions....

  8. Yrityssaneerausmenettelyn hoitaminen Asianajotoimisto Saloranta Lunkka Pohja ja Kopra Oy:ssä

    OpenAIRE

    Räisänen, Christina

    2012-01-01

    TIIVISTELMÄ KYMENLAAKSON AMMATTIKORKEAKOULU Liiketalouden koulutusohjelma / Juridiikan suuntautumisvaihtoehto RÄISÄNEN, CHRISTINA Yrityssaneerausmenettelyn hoitaminen Asianajotoimisto Saloranta Lunkka Pohja ja Kopra Oy:ssä Opinnäytetyö 55 sivua + 50 liitesivua Työn ohjaaja Lehtori Ollila, Päivi Toimeksiantaja Asianajotoimisto Saloranta Lunkka Pohja ja Kopra Oy Huhtikuu 2012 Avainsanat yrityssaneeraus, saneerausmenettely, selvittäjä, valvoja Laki yrityksen s...

  9. Liiketalous kulttuurituottajien opiskelussa ja työssä : Näkökulmia Humakin opetussuunnitelmauudistukseen

    OpenAIRE

    Jokinen, Sini

    2013-01-01

    Opinnäytetyön tilaajana toimi Humanistinen ammattikorkeakoulu. Tutkimuksen tavoitteena oli selvittää, millaiseksi Humakin kulttuurituotannon opiskelijat ja koulutusohjelmasta jo valmistuneet kulttuurituottajat ovat kokeneet liiketaloudellisen opetuksen. Tutkin työssäni tulisiko Humakin lisätä liiketaloudellisia opintoja tulevaan opetussuunnitelmaan, ja miten opetusta voisi kehittää. Uusi opetussuunnitelma otetaan käyttöön syksyllä 2013. Liiketalousosaamisen merkitys tuntuu kasvavan kulttu...

  10. Immune Responses of Specific-Pathogen-Free Mice to Chronic Helicobacter pylori (Strain SS1) Infection

    OpenAIRE

    Ferrero, Richard L.; Thiberge, Jean-Michel; Huerre, Michel; Labigne, Agnès

    1998-01-01

    A model permitting the establishment of persistent Helicobacter pylori infection in mice was recently described. To evaluate murine immune responses to H. pylori infection, specific-pathogen-free Swiss mice (n = 50) were intragastrically inoculated with 1.2 × 107 CFU of a mouse-adapted H. pylori isolate (strain SS1). Control animals (n = 10) received sterile broth medium alone. Animals were sacrificed at various times, from 3 days to 16 weeks postinoculation (p.i.). Quantitative culture of ga...

  11. Novel ssDNA viruses discovered in yellow-crowned parakeet (Cyanoramphus auriceps) nesting material.

    Science.gov (United States)

    Sikorski, Alyssa; Kearvell, Jonathan; Elkington, Simon; Dayaram, Anisha; Argüello-Astorga, Gerardo R; Varsani, Arvind

    2013-07-01

    During routine monitoring of yellow-crowned parakeets in the Poulter Valley of the South Island of New Zealand, a dead parakeet chick was discovered in a nest. Known parrot-infecting viruses, such as beak and feather disease virus (BFDV), avian polyomavirus (APV), and parrot hepatitis B virus (PHBV), were not detected in the nesting material. However, we recovered two novel single-stranded DNA viruses (ssDNA), CynNCXV (2308 nt) and CynNCKV (2087 nt), which have genome architectures similar to those of circoviruses, characterised by circular genomes with two large bidirectional open reading frames (ORFs). Both contain a stem-loop element with a conserved nonanucleotide motif, known to be required for rolling-circle replication. The full genomes had no BLASTn similarity to known ssDNA viruses. However, in both genomes the larger ORFs have BLAST similarity to known replication-associated proteins (Reps). CynNCKV has 30 % similarity to picobiliphyte nano-like virus (Picobiliphyte M5584-5) with 66-88 % coverage (e-value of 5×10(-33)), whereas CynNCXV has 33 % similarity to rodent stool-associated virus (RodSCV M-45) with 92-94 % coverage (e-value of 5 × 10(-31)). Found within these ORFs were the rolling-circle replication motifs I, II, III and the helicase motifs Walker A and Walker B. Maximum-likelihood phylogenetic analysis of the Reps reveals that these are two novel ssDNA viruses. At this point, we are unable to attribute the death of the parakeet to these two new novel ssDNA viruses. PMID:23417396

  12. Fraïssé-Hrushovski predimensions on nilpotent Lie algebras

    OpenAIRE

    Amantini, Andrea

    2011-01-01

    In dieser Arbeit wird das Fraïssé-Hrushowskis Amalgamationsverfahren in Zusammenhang mit nilpotenten graduierten Lie Algebren über einem endlichen Körper untersucht. Die Prädimensionen die in der Konstruktion auftauchen sind mit dem gruppentheoretischen Begriff der Defizienz zu vergleichen, welche auf homologische Methoden zurückgeführt werden kann. Darüber hinaus wird die Magnus-Lazardsche Korrespondenz zwischen den oben genannten Lie Algebren und nilpotenten Gruppen von Primzahl-Exponente...

  13. Echelle-MEPSICRON time-resolved spectroscopy of the dwarf nova SS Cygni

    International Nuclear Information System (INIS)

    High-dispersion time-resolved spectroscopy of the dwarf nova SS Cygni was obtained during two consecutive days at the end of an outburst. The emission lines present a double-peaked profile with a peak-to-peak separation of 526±14 km s-1. The lines have a complex structure which includes a narrow asymmetric component coming from a hot spot in the accretion disc or from the chromosphere of the red dwarf. (author)

  14. Echelle-MEPSICRON time-resolved spectroscopy of the dwarf nova SS Cygni

    Energy Technology Data Exchange (ETDEWEB)

    Echevarria, J.; Tapia, M. (Universidad Nacional Autonoma de Mexico, Baja California (Mexico). Inst. de Astronomia); Diego, F. (Institute of Oceanographic Sciences, Godalming (UK)); Costero, R.; Ruiz, E.; Salas, L.; Gutierrez, L.; Enriquez, R. (Universidad Nacional Autonoma de Mexico, Mexico City (Mexico). Inst. de Astronomia)

    1989-10-15

    High-dispersion time-resolved spectroscopy of the dwarf nova SS Cygni was obtained during two consecutive days at the end of an outburst. The emission lines present a double-peaked profile with a peak-to-peak separation of 526{plus minus}14 km s{sup -1}. The lines have a complex structure which includes a narrow asymmetric component coming from a hot spot in the accretion disc or from the chromosphere of the red dwarf. (author).

  15. Imetysohjaus lastenneuvolan terveydenhoitajan työssä : terveydenhoitajille suunnatun imetysohjaajakoulutustarpeen kartoitus

    OpenAIRE

    Willis, Merete

    2009-01-01

    Tämän opinnäytetyön tarkoituksena oli kartoittaa terveydenhoitajille suunnatun imetysohjaajakoulutuksen tarvetta tutkimalla lastenneuvolassa työskentelevien terveydenhoitajien mielipiteitä heille mahdollisen suunnatun koko imetystaipaletta käsittelevää imetysohjauskoulutuksen tarpeesta. Lisäksi seurattiin lastenneuvolan terveydenhoitajan työssä esiin tulleita imetysohjaustarpeita. Viitekehyksenä toimivat 1) Imetyksen tuki ry:n valtakunnalliseen Imetystukipuhelimeen vuonna 2006 tulleiden y...

  16. Geological notes and local details for geological sheet SS91SE (Tiverton)

    OpenAIRE

    Lee, Jonathan R.

    2005-01-01

    This report describes and provides an initial interpretation of the bedrock and superficial geology of 1:10, 000 Geological Sheet SS 91 SE Tiverton (Devon). The geology of the area consists of Carboniferous sandstones and mudstones, which belong to the Bude Formation (Culm), in-turn these are unconformably overlain by Permian sandstone and breccias. The sandstone, typically a fine-grained sandstone or sandy mudstone, is informally called the ‘Tidcombe Sands’, whilst two breccias can be recogn...

  17. Intermittency in 200 GeV/nucleon S+S collisions

    International Nuclear Information System (INIS)

    We have studied one and two-dimensional intermittency in S+S collisions at 200 GeV/nucleon in a high statistics electronic measurement at the CERN SPS using pad-readout streamer tubes. We observe no intermittency signal beyond that produced by folding the Fritiof event generator with a detailed model of our detector. Even though the observed signal contains significant distortions due to experimental effects, we show that we are sensitive to intermittency in the collision. (orig.)

  18. Defining the Content of an Online Sexual Health Intervention: The MenSS Website

    OpenAIRE

    Webster, R; Gerressu, M; Michie, S; Estcourt, C.; Anderson, J.; Ang, C S; Murray, E; Rait, G.; Stephenson, J; Bailey, J. V.

    2015-01-01

    BACKGROUND: Health promotion and risk reduction are essential components of sexual health care. However, it can be difficult to prioritize these within busy clinical services. Digital interventions may provide a new method for supporting these. OBJECTIVE: The MenSS (Men's Safer Sex) website is an interactive digital intervention developed by a multidisciplinary team, which aims to improve condom use in men who have sex with women (MSW). This paper describes the content of this intervention, a...

  19. Intermittency in 200 GeV/nucleon S+S collisions

    International Nuclear Information System (INIS)

    We have studied one and two-dimensional intermittency in S+S collisions at 200 GeV/nucleon in a high statistics electronic measurement at the CERN SPS using pad-readout streamer tubes. We observe no intermittency signal beyond that produced by folding the Fritiof event generator with a detailed model of our detector. Even though the observed signal contains significant distortions due to experimental effects, we show that we are sensitive to intermittency in the collision

  20. Programmability and portability for exascale: Top down programming methodology and tools with StarSs

    OpenAIRE

    Subotic, Vladimir; Brinkmann, Steffen; Marjanovic, Vladimir; Badia, Rosa M.; Gracia, Jose; Niethammer, Christoph; Ayguade, Eduard; LABARTA, Jesus; Valero, Mateo

    2013-01-01

    StarSs is a task-based programming model that allows to parallelize sequential applications by means of annotating the code with compiler directives. The model further supports transparent execution of designated tasks on heterogeneous platforms, including clusters of GPUs. This paper focuses on the methodology and tools that complements the programming model forming a consistent development environment with the objective of simplifying the live of application developers.The programming envir...

  1. Implementing OmpSs support for regions of data in architectures with multiple address spaces

    OpenAIRE

    Bueno J.; Martorell X; Badia R.M.; Ayguade E.; Labarta J.

    2013-01-01

    The need for features for managing complex data accesses in modern programming models has increased due to the emerging hardware architectures. HPC hardware has moved towards clusters of accelerators and/or multicores, architectures with a complex memory hierarchy exposed to the programmer. We present the implementation of data regions on the OmpSs programming model, a high-productivity annotation-based programming model derived from OpenMP. This enables the programmer to specify regions of s...

  2. Naisten karkkikaupassa : kokemuksia myynnin ammattitutkinnosta kosmetiikan face to face -myyntityössä

    OpenAIRE

    Alasiurua, Riikka

    2012-01-01

    Kosmetiikan jälleenmyynti on uudessa tilanteessa kosmetiikkatuotteiden saatavuuden laajennuttua Internetin maailmaan. Myös kosmetiikkaan liittyvä informaatio on helposti kuluttajien ulottuvilla. Näin ollen kuluttajat eivät ole enää riippuvaisia kosmetiikkamyyjien osaamisesta. Koska verkkokauppojen hintataso on usein perinteisiä jälleenmyyntipisteitä alhaisempi, ainoa kilpailuetu kasvokkain tapahtuvassa myyntityössä on vuorovaikutus. Sen tärkeys korostuu erityisesti kosmetiikan myynnissä...

  3. Complete genome sequence of Treponema pallidum ssp. pallidum strain SS14 determined with oligonucleotide arrays

    OpenAIRE

    Sodergren Erica; Petrosino Joseph F; Palzkill Timothy; Norris Steven J; Šmajs David; Strouhal Michal; Matějková Petra; Norton Jason E; Singh Jaz; Richmond Todd A; Molla Michael N; Albert Thomas J; Weinstock George M

    2008-01-01

    Abstract Background Syphilis spirochete Treponema pallidum ssp. pallidum remains the enigmatic pathogen, since no virulence factors have been identified and the pathogenesis of the disease is poorly understood. Increasing rates of new syphilis cases per year have been observed recently. Results The genome of the SS14 strain was sequenced to high accuracy by an oligonucleotide array strategy requiring hybridization to only three arrays (Comparative Genome Sequencing, CGS). Gaps in the resultin...

  4. Evidence for 4-chlorobenzoic acid dehalogenation mediated by plasmids related to pSS50.

    OpenAIRE

    Layton, A C; Sanseverino, J; Wallace, W; Corcoran, C; Sayler, G. S.

    1992-01-01

    The biodegradation of 4-chlorobiphenyl usually proceeds through the intermediate 4-chlorobenzoate. Few bacterial strains can degrade 4-chlorobiphenyl to 4-chlorobenzoate and 4-chlorobenzoate to CO2. This study demonstrates that the 4-chlorobiphenyl-degrading Alcaligenes sp. strain ALP83 can degrade 4-chlorobenzoate to 4-hydroxybenzoate. The dehalogenase activity is correlated with a 10-kb fragment carried on plasmid pSS70.

  5. On the Hubble Space Telescope Trigonometric Parallax of the Dwarf Nova SS Cygni

    CERN Document Server

    Nelan, Edmund P

    2013-01-01

    SS Cygni is one of the brightest dwarf novae (DNe), and one of the best-studied prototypes of the cataclysmic variables. Astrometric observations with the Fine Guidance Sensors (FGS) on the Hubble Space Telescope (HST), published in 2004, gave an absolute trigonometric parallax of 6.06+/-0.44 mas. However, recent very-long-baseline interferometry (VLBI), obtained during radio outbursts of SS Cyg, has yielded a significantly larger absolute parallax of 8.80+/-0.12 mas, as well as a large difference in the direction of the proper motion compared to the HST result. The VLBI distance reduces the implied luminosity of SS Cyg by about a factor of two, giving good agreement with predictions based on accretion-disk theory in order to explain the observed DN outburst behavior. This discrepancy raises the possibility of significant systematic errors in FGS parallaxes and proper motions. We have reanalyzed the archival HST/FGS data, including (1) a critical redetermination of the parallaxes of the background astrometric...

  6. Exploring optimization parameters to increase ssDNA recombineering in Lactococcus lactis and Lactobacillus reuteri.

    Science.gov (United States)

    Van Pijkeren, Jan-Peter; Neoh, Kar Mun; Sirias, Denise; Findley, Anthony S; Britton, Robert A

    2012-01-01

    Single-stranded DNA (ssDNA) recombineering is a technology which is used to make subtle changes in the chromosome of several bacterial genera. Cells which express a single-stranded DNA binding protein (RecT or Bet) are transformed with an oligonucleotide which is incorporated via an annealing and replication-dependent mechanism. By in silico analysis we identified ssDNA binding protein homologs in the genus Lactobacillus and Lactococcus lactis. To assess whether we could further improve the recombineering efficiency in Lactobacillus reuteri ATCC PTA 6475 we expressed several RecT homologs in this strain. RecT derived from Enterococcus faecalis CRMEN 19 yielded comparable efficiencies compared with a native RecT protein, but none of the other proteins further increased the recombineering efficiency. We successfully improved recombineering efficiency 10-fold in L. lactis by increasing oligonucleotide concentration combined with the use of oligonucleotides containing phosphorothioate-linkages (PTOs). Surprisingly, neither increased oligonucleotide concentration nor PTO linkages enhanced recombineering in L. reuteri 6475. To emphasize the utility of this technology in improving probiotic features we modified six bases in a transcriptional regulatory element region of the pdu-operon of L. reuteri 6475, yielding a 3-fold increase in the production of the antimicrobial compound reuterin. Directed genetic modification of lactic acid bacteria through ssDNA recombineering will simplify strain improvement in a way that, when mutating a single base, is genetically indistinguishable from strains obtained through directed evolution. PMID:22750793

  7. A novel, highly divergent ssDNA virus identified in Brazil infecting apple, pear and grapevine.

    Science.gov (United States)

    Basso, Marcos Fernando; da Silva, José Cleydson Ferreira; Fajardo, Thor Vinícius Martins; Fontes, Elizabeth Pacheco Batista; Zerbini, Francisco Murilo

    2015-12-01

    Fruit trees of temperate and tropical climates are of great economical importance worldwide and several viruses have been reported affecting their productivity and longevity. Fruit trees of different Brazilian regions displaying virus-like symptoms were evaluated for infection by circular DNA viruses. Seventy-four fruit trees were sampled and a novel, highly divergent, monopartite circular ssDNA virus was cloned from apple, pear and grapevine trees. Forty-five complete viral genomes were sequenced, with a size of approx. 3.4 kb and organized into five ORFs. Deduced amino acid sequences showed identities in the range of 38% with unclassified circular ssDNA viruses, nanoviruses and alphasatellites (putative Replication-associated protein, Rep), and begomo-, curto- and mastreviruses (putative coat protein, CP, and movement protein, MP). A large intergenic region contains a short palindromic sequence capable of forming a hairpin-like structure with the loop sequence TAGTATTAC, identical to the conserved nonanucleotide of circoviruses, nanoviruses and alphasatellites. Recombination events were not detected and phylogenetic analysis showed a relationship with circo-, nano- and geminiviruses. PCR confirmed the presence of this novel ssDNA virus in field plants. Infectivity tests using the cloned viral genome confirmed its ability to infect apple and pear tree seedlings, but not Nicotiana benthamiana. The name "Temperate fruit decay-associated virus" (TFDaV) is proposed for this novel virus. PMID:26186890

  8. Electrical Properties of Al, Ag, Cu, Ti and SS Thin Film for Electrode of Solar Cell

    International Nuclear Information System (INIS)

    The Al, Ag, Cu, Ti and SS materials were deposited on the surface of glass substrate using plasma DC sputtering technique. The deposition process was done with the following plasma parameters : deposition time, gas pressure and substrate temperature with the aim to obtain a good conductance of thin films. Variation of substrate deposition time was 1 - 15 minutes, gas pressure was 5x10-2 - 7x10-2 torr and of temperature was 100 - 300 oC. The resistance measurement has been done by four points probes and the conductivity was calculated using mathematic formulation. It was obtained that the minimum resistance in the order of R = 0.07 Ω, was found at Ag materials and this was obtained at the following plasma parameters : deposition time 15 minutes, gas pressure 6x10-2 torr and temperature 300 oC, while, the resistance of : Cu, Al, Ti and SS materials were R = 0.13 Ω, R = 450 Ω, R = 633 Ω, R = 911 Ω respectively, It could be concluded that the Ag thin film has a minimum resistance, high conductivity compared to the other materials Al, Cu, Ti and SS. Ag is therefore the suitable material for applying as electrode of solar cell. (author)

  9. Evaluation of hydroxyapatite coatings on borate passivated 316L SS in Ringer's solution

    International Nuclear Information System (INIS)

    Surgical grade stainless steel (316L SS) is one of the widely used implant material in orthopedic surgeries. But often the release of metal ions is evidenced from the implants and subsequently a second surgery is required to remove the implant material. One way to control this release of metal ions is to coat the implant material with a biocompatible material like hydroxyapatite. In this paper we have reported a successful coating of hydroxyapatite over borate passivated 316L SS by a dip coating method. The coatings were characterized by electrochemical techniques such as potentiodynamic polarization, electrochemical impedance and cyclic voltammetry (CV). Also X-ray diffraction (XRD) and scanning electron microscopic (SEM) studies were performed to confirm the quality of the coatings. Results of accelerated leach out characteristics by inductively coupled plasma-atomic emission spectroscopy (ICP-AES) and the evaluation of shear strength are also presented to support the corrosion resistant nature of the coatings. The enhanced bio-resistivity of the as-formed HAP coatings on passivated 316L SS is attributed to the protective passive layer formed by borate buffer solution at selected potentials.

  10. Characterization of mixed monolayers of phosphatidylcholine and a dicationic gemini surfactant SS-1 with a langmuir balance: effects of DNA.

    Science.gov (United States)

    Matti, V; Säily, J; Ryhänen, S J; Holopainen, J M; Borocci, S; Mancini, G; Kinnunen, P K

    2001-01-01

    Monolayers of a cationic gemini surfactant, 2,3-dimethoxy-1,4-bis(N-hexadecyl-N;N-dimethyl-ammonium)butane dibromide (abbreviated as SS-1) and its mixtures with 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) were studied using a Langmuir balance. More specifically, we measured the force-area (pi-A) curves and determined the elastic area compressibility modulus (C) as a function of lateral packing pressure and the mole fraction of the cationic lipid (X(SS-1)), with and without DNA in the subphase. Both SS-1 and POPC exhibited smooth compression isotherms, indicating their monolayers to be in the liquid expanded state. Even low contents (X(SS-1) 0.5. At a higher concentration of DNA (1.88 microM base pairs) in the subphase corresponding to DNA/SS-1 charge ratio of 3.75 at X(SS-1) = 0.5, condensation was observed at all values of X(SS-1). PMID:11566784

  11. The fusion protein SS18-SSX1 employs core Wnt pathway transcription factors to induce a partial Wnt signature in synovial sarcoma.

    Science.gov (United States)

    Cironi, Luisa; Petricevic, Tanja; Fernandes Vieira, Victor; Provero, Paolo; Fusco, Carlo; Cornaz, Sandrine; Fregni, Giulia; Letovanec, Igor; Aguet, Michel; Stamenkovic, Ivan

    2016-01-01

    Expression of the SS18/SYT-SSX fusion protein is believed to underlie the pathogenesis of synovial sarcoma (SS). Recent evidence suggests that deregulation of the Wnt pathway may play an important role in SS but the mechanisms whereby SS18-SSX might affect Wnt signaling remain to be elucidated. Here, we show that SS18/SSX tightly regulates the elevated expression of the key Wnt target AXIN2 in primary SS. SS18-SSX is shown to interact with TCF/LEF, TLE and HDAC but not β-catenin in vivo and to induce Wnt target gene expression by forming a complex containing promoter-bound TCF/LEF and HDAC but lacking β-catenin. Our observations provide a tumor-specific mechanistic basis for Wnt target gene induction in SS that can occur in the absence of Wnt ligand stimulation. PMID:26905812

  12. Melanoma inhibitor of apoptosis protein (ML-IAP) specific cytotoxic T lymphocytes cross-react with an epitope from the auto-antigen SS56

    DEFF Research Database (Denmark)

    Baek Sørensen, Rikke; Faurschou, Mikkel; Troelsen, Lone; Schrama, David; Jacobsen, Søren; Becker, Jürgen C; Thor Straten, Per; Andersen, Mads Hald

    2009-01-01

    described target of autoantibody responses in Sjögren's syndrome (SS) as well as systemic lupus erythematosus (SLE). Here, we describe that SS56 is also an auto-antigen for T cells in SS and SLE. Hence, SS56-specific T cells could readily be detected in circulation and among the infiltrating cells of SLE...... skin lesions. SS56-specific T cells were able to lyse target cells presenting the peptide epitope on the surface. Notably, SS56-specific CD8 T cells isolated from an SS patient cross reacted with the ML-IAP epitope. This early evidence of a target for auto-reactive CTL in SS and SLE patients; it is to...

  13. Synthesis of Novel High Energy Polyaniline Derivative Containing S-S Bond in Six-membered Cycle

    Institute of Scientific and Technical Information of China (English)

    ZHOU Lei; DENG Shi-Ren; LI Zao-Ying

    2003-01-01

    @@ A novel conducting polymer as new class of high energy storage material, [1,2] which has one six-membered cycle containing S-S bond in the moiety of aniline was synthesized. The polymer had enhanced redox progress due to an intramolecular electrocatalytic effect of polyaniline on S-S bond. The polymer has some advantages because of its high theoretical specific energy and fast kinetics when used in lithium batteries. The most promised advantage of it is that the intramolecular cleavage and recombination of the S-S bond improve the cyclicability greatly.

  14. Porkkanapesätoiminta lasten kokemana : "Porkkanapesässä on kivaa, kun saa olla kaikkien kanssa"

    OpenAIRE

    Painokallio, Sanna; Viitanen, Leena

    2006-01-01

    Opinnäytetyössä kartoitettiin Mannerheimin Lastensuojeluliiton Satakunnan piirin järjestämän porkkanapesätoiminnan merkitystä ja roolia Pormestarinluodon lasten elämässä. Tutkimuksessa huomioitiin myös lasten perheiden näkökulmat Porkkanapesän tarjoamasta toiminnasta. Tutkimus tehtiin yhteistyössä Mannerheimin Lastensuojeluliiton kanssa. Tavoitteena oli hankkia porkkanapesätoiminnasta ajantasaista tietoa ja huomioida erityisesti lasten mielipiteet. Tutkimusmenetelmänä käytettiin kvalitatiivis...

  15. Binding of antibodies to the extractable nuclear antigens SS-A/Ro and SS-B/La is induced on the surface of human keratinocytes by ultraviolet light (UVL): Implications for the pathogenesis of photosensitive cutaneous lupus

    International Nuclear Information System (INIS)

    Autoantibodies to the non-histone nucleoprotein antigens SS-A/Ro, SS-B/La, and RNP are highly associated with photosensitive cutaneous lupus erythematosus (LE). In order to better understand the potential mechanisms of ultraviolet (UV) light on photosensitivity in patients with cutaneous LE, we designed immunopathologic in vitro and in vivo experiments to evaluate the effects of UV on the binding of such autoantibodies to the surface of human keratinocytes, one major target of immunologic damage in photosensitive LE. Short-term 2% paraformaldehyde fixation of suspensions of cultured human keratinocytes previously incubated with monospecific antiserum probes enabled the detection of ENA expression on the cell surface by flow-cytometry analysis. UVB light (280-320 nm) induced the binding of monospecific antibody probes for SS-A/Ro and SS-B/La on keratinocytes in a dose-dependent pattern with maximal induction observed at the dose of 200 mJ/cm2 UVB. Binding of SS-A/Ro, SS-B/La, and RNP antibody was augmented strongly, but binding of anti-Sm was very weak. In contrast, UVA (320-400 nm) light had no effect on the induction of binding of these antibody probes. Identical results were seen by standard immunofluorescence techniques. Hydroxyurea-treated keratinocytes showed similar induction of those antigens by UVB irradiation, which suggested that ENA expression on cultured keratinocytes by UVB were cell-cycle independent. Tunicamycin, an inhibitor of glycosylation of proteins, reduced UVB light effect on the SS-A/Ro and SS-B/La antigen's expression. These in vitro FACS analyses revealed that ENA augmentation on the keratinocyte cell surface was dose dependent, UVB dependent, glycosylation dependent, and cell-cycle independent. In vivo ENA augmentation on the keratinocyte surface was examined in suction blister epidermal roofs

  16. Binding of antibodies to the extractable nuclear antigens SS-A/Ro and SS-B/La is induced on the surface of human keratinocytes by ultraviolet light (UVL): Implications for the pathogenesis of photosensitive cutaneous lupus

    Energy Technology Data Exchange (ETDEWEB)

    Furukawa, F.; Kashihara-Sawami, M.; Lyons, M.B.; Norris, D.A. (Univ. of Colorado School of Medicine, Denver (USA))

    1990-01-01

    Autoantibodies to the non-histone nucleoprotein antigens SS-A/Ro, SS-B/La, and RNP are highly associated with photosensitive cutaneous lupus erythematosus (LE). In order to better understand the potential mechanisms of ultraviolet (UV) light on photosensitivity in patients with cutaneous LE, we designed immunopathologic in vitro and in vivo experiments to evaluate the effects of UV on the binding of such autoantibodies to the surface of human keratinocytes, one major target of immunologic damage in photosensitive LE. Short-term 2% paraformaldehyde fixation of suspensions of cultured human keratinocytes previously incubated with monospecific antiserum probes enabled the detection of ENA expression on the cell surface by flow-cytometry analysis. UVB light (280-320 nm) induced the binding of monospecific antibody probes for SS-A/Ro and SS-B/La on keratinocytes in a dose-dependent pattern with maximal induction observed at the dose of 200 mJ/cm2 UVB. Binding of SS-A/Ro, SS-B/La, and RNP antibody was augmented strongly, but binding of anti-Sm was very weak. In contrast, UVA (320-400 nm) light had no effect on the induction of binding of these antibody probes. Identical results were seen by standard immunofluorescence techniques. Hydroxyurea-treated keratinocytes showed similar induction of those antigens by UVB irradiation, which suggested that ENA expression on cultured keratinocytes by UVB were cell-cycle independent. Tunicamycin, an inhibitor of glycosylation of proteins, reduced UVB light effect on the SS-A/Ro and SS-B/La antigen's expression. These in vitro FACS analyses revealed that ENA augmentation on the keratinocyte cell surface was dose dependent, UVB dependent, glycosylation dependent, and cell-cycle independent. In vivo ENA augmentation on the keratinocyte surface was examined in suction blister epidermal roofs.

  17. Ihminen suunnittelun lähtökohtana : palvelumuotoilu Meilahden hammashoitolan kehittämistyössä

    OpenAIRE

    Aukia, Matti

    2014-01-01

    ssä opinnäytetyössä selvitetään miten palvelumuotoilua voidaan hyödyntää Meilahden hammashoitolan kehittämistyössä. Kirjoittaja on paitsi valmistuva teollinen muotoilija, myös hammaslääkäri. Tämä näkökulma antaa kehittämistyöhön oman kansanterveyttä ja toisaalta työntekijää korostavan erityispiirteensä. Meilahden hammashoitola on keväällä 2014 käynnistetty hanke, jossa on tarkoituksena kokeilla laajan hoidon tarpeessa olevien aikuisten hoitamista ripeässä tahdissa moniammatillisesti työsken...

  18. Mechanical properties of Cu sbnd Cr sbnd Zr alloy and SS316 joints fabricated by friction welding method

    Science.gov (United States)

    Tsuchiya, Kunihiko; Kawamura, Hiroshi

    1996-10-01

    Copper alloys with high-strength and high-conductivity are being considered for several magnetic fusion energy applications such as the first wall in high power-density devices, resistive magnetic coils, and high-heat flux components. For example, the stainless steel is a structural material while Cu-alloy acts as a heat sink material for the surface heat flux in the first wall. Therefore, development of reliable joints between Cu-alloys and stainless steel (SS316) is required. In the present work, joining tests on Cu—1%Cr—1%Zr/SS316 by friction welding were performed, and optimum fabricating conditions of the Cu-alloy/SS316 joint were determined. Additionally, the characteristics of tensile strength, hardness, metallographical observation and SEM/EPMA analyses on Cu—1%Cr—1%Zr/SS316 fabricated by friction welding were evaluated.

  19. Vuoden 2005 laaja eläkeuudistus ja ikääntyvien työssä jatkamisaikeet

    OpenAIRE

    Kiuru, Jaana

    2006-01-01

    ssä opinnäytetyössä on käsitelty vuoden 2005 alusta voimaan tullutta yksityisen sektorin työeläkeuudistusta, jonka tärkeimpänä tavoitteena on ollut nostaa keskimääräistä eläkkeelle siirtymisikää muutamalla vuodella. Tarkoituksena oli selvittää, mitä muutoksia uudistus toi työeläketurvaan. Lisäksi pyrittiin selvittämään, miten työeläketurvaan tehdyt muutokset vaikuttavat eläkeikää lähestyvien työntekijöiden työssä jatkamiseen ja toisaalta eläkkeelle siirtymiseen. Opinnäytetyössä kartoitettii...

  20. Työntekijän oikeus yksityisyyteen työelämässä

    OpenAIRE

    Vepsäläinen, Ninja

    2010-01-01

    Tämän opinnäytetyön tavoite oli antaa kattava selvitys työntekijän oikeudesta yksityisyyteen työelämässä sekä arvioida sitä, toteutuuko työntekijän oikeus yksityisyyteen työelämässä. Työni perustuu työntekijän oikeudesta yksityisyyden suojaan työelämässä annettuun lakiin, henkilötietolakiin ja yhteistoimintalakiin. Käsittelen työssäni ajankohtaisia työntekijän yksityisyyteen liittyviä kysymyksiä, työntekijöistä kerättäviä henkilötietoja, työntekijöiden teknistä valvontaa, työantajan oikeutta ...

  1. Investigation on fracture behavior of the welded joint HAZ of ultra-fine grain steel SS400

    Institute of Scientific and Technical Information of China (English)

    朱政强; 陈立功; 荆洪阳; 葛景国; 倪纯珍; 饶德林

    2003-01-01

    The critical crack dimensions of both base-metal specimen and HAZ specimen are measured via wide-plate tensile tests. Based on the "fitness for purpose" principle, the fracture behavior of the ultra-fine grain steel SS400 welded joint HAZ is assessed. The test results indicate that overmatching is benefit for the whole capability's improvement of ultra-fine grain steel SS400. The test results are confirmed by using finite element method (FEM).

  2. Highlights from the Functional Single Nucleotide Polymorphisms Associated with Human Muscle Size and Strength or FAMuSS Study

    OpenAIRE

    Pescatello, Linda S.; Devaney, Joseph M.; Hubal, Monica J; Thompson, Paul D.; Hoffman, Eric P

    2013-01-01

    The purpose of the Functional Single Nucleotide Polymorphisms Associated with Human Muscle Size and Strength study or FAMuSS was to identify genetic factors that dictated the response of health-related fitness phenotypes to resistance exercise training (RT). The phenotypes examined were baseline muscle strength and muscle, fat, and bone volume and their response to RT. FAMuSS participants were 1300 young (24 years), healthy men (42%) and women (58%) that were primarily of European-American de...

  3. The eSS rat, a nonobese model of disordered glucose and lipid metabolism and fatty liver

    OpenAIRE

    Daniele Stella M; Montenegro Silvana M.; Tarres María C; Picena Juan C; Martinez Stella M

    2010-01-01

    Abstract Background eSS is a rat model of type 2 diabetes characterized by fasting hyperglycemia, glucose intolerance, hyperinsulinemia and early hypertriglyceridemia. Diabetic symptoms worsen during the second year of life as insulin release decreases. In 12-month-old males a diffuse hepatic steatosis was detected. We report the disturbances of lipid metabolism of the model with regard to the diabetic syndrome. Methods The study was conducted in eight 12-month-old eSS male rats and seven age...

  4. Kouluikäisen seksuaaliterveyskasvatusmateriaali historiasta tulevaisuuteen : materiaalien käyttö terveydenhoitajan työssä

    OpenAIRE

    Paajanen, Heini; Perttula, Eliisa

    2015-01-01

    Tämä opinnäytetyö on osa Metropolian Ammattikorkeakoulun Hoitotyön koulutuksen museon hanketta. Hankeen tavoitteena on kuvata suomalaisen hoitotyön historiaa. Opinnäytetyömme aiheena on seksuaaliterveyskasvatus kouluissa historiasta nykyaikaan, erikoistarkastelussa seksuaaliterveyskasvatusmateriaalien käyttö kouluterveydenhoitajan työssä. Opinnäytetyö on toteutettu vuonna 2015. Opinnäytetyössä vastaamme siihen millaista seksuaalikasvatus ja seksuaalikasvatusmateriaalit olivat 1900-luvulta...

  5. Developmental patterns of GHr and SS mRNA expression in porcine gastric tissue

    Institute of Scientific and Technical Information of China (English)

    Dong Xia; Ru-Qian Zhao; Xi-Hui Wei; Qing-Fu Xu; Jie Chen

    2003-01-01

    AIM: The present study was aimed to investigate the developmental patterns of growth hormone receptor (GHr)and somatostatin (SS) mRNA expression in porcine gastric tissue and its relationship with gastric growth and gastric functional development.METHODS: Erhualian and Large White boars were selected randomly and sampled at birth (DO), 3, 20, 30, 90, 120 and 180 days of age respectively, meanwhile the bodyweight and gastric weight were recorded. The single tube semiquantitative RT-PCR was applied in this experiment to investigate the developmental patterns of gastric GHr and SS mRNA expression, the correlations between the patterns of mRNA expression and the relative gastric weight (ratio of gastric weight to bodyweight) and the pepsin contents in gastric mucous membrane were analyzed. In order to further elucidate the effect of GH on gastric function, the primary cultures of gastric fundic mucosal cells were treated with 2ng/ml, 20 ng/ml and 200 ng/ml of rpGH for 18 hrs respectively,and the pepsin contents in culture medium were measured.RESULTS: The expression of GHr mRNA was high at birth,followed by a significant decrease at 3 days of age (D3) in both breeds. In Large White boars, the expression of GHr mRNA reached a peak at D90 and remained a plateau afterward. In Erhualian pigs, however, a slight yet significant increase occurred at D30 reaching a level that was kept constant thereafter. From birth to D30, the expression of GHr mRNA in gastric tissue was higher in Erhualian boars than that in Large White boars, but from D90 to D180, the higher expression of GHr mRNA was found in Large White boars.The gastric GHr mRNA expression was significantly correlated with the relative gastric weight (r=0.541, P<0.05) and pepsin content in gastric mucosa (r=0.625, P<0.05) respectively.The gastric SS mRNA expression was high at birth (Erhualian 1.59, Large White 0.80), but dropped significantly at D3 (Erhualian 0.95, Large White 0.19, P<0.05), a stepwise increase

  6. The simultaneous production of sphingan Ss and poly(R-3-hydroxybutyrate) in Sphingomonas sanxanigenens NX02.

    Science.gov (United States)

    Wu, Mengmeng; Li, Guoqiang; Huang, Haidong; Chen, Sibin; Luo, Ying; Zhang, Wenwen; Li, Keran; Zhou, Jiefang; Ma, Ting

    2016-01-01

    Sphingans and poly(R-3-hydroxybutyrate) (PHB) are both widely used biopolymers produced by bacteria. In the batch fermentation of Sphingomonas sanxanigenens NX02 in a 5L fermenter using glucose as carbon source, ivory colored sphingan Ss production was a growth-associated process with a maximum purified production of 14.88 ± 0.83 g/L, while 6.08 ± 0.23 g/L PHB was simultaneously produced. Sphingan Ss and PHB were separated by a simple dilution, heating and centrifugation or filtration process, and sphingan Ss can be cost-effectively extracted using a small amount of acid rather than multi-fold volumes of alcohols. From ultrathin sections of S. sanxanigenens NX02, we found that the interior space of the cells was filled with PHB granules, and the outside was surrounded by abundant Ss. The purified sphingan Ss can be used as an excellent gelling and emulsifying agent in biotechnology applications such as food, personal care and production processes. Proposed pathways of Ss and PHB biosynthesis from glucose are also presented. PMID:26434528

  7. ssODN-mediated knock-in with CRISPR-Cas for large genomic regions in zygotes

    Science.gov (United States)

    Yoshimi, Kazuto; Kunihiro, Yayoi; Kaneko, Takehito; Nagahora, Hitoshi; Voigt, Birger; Mashimo, Tomoji

    2016-01-01

    The CRISPR-Cas system is a powerful tool for generating genetically modified animals; however, targeted knock-in (KI) via homologous recombination remains difficult in zygotes. Here we show efficient gene KI in rats by combining CRISPR-Cas with single-stranded oligodeoxynucleotides (ssODNs). First, a 1-kb ssODN co-injected with guide RNA (gRNA) and Cas9 messenger RNA produce GFP-KI at the rat Thy1 locus. Then, two gRNAs with two 80-bp ssODNs direct efficient integration of a 5.5-kb CAG-GFP vector into the Rosa26 locus via ssODN-mediated end joining. This protocol also achieves KI of a 200-kb BAC containing the human SIRPA locus, concomitantly knocking out the rat Sirpa gene. Finally, three gRNAs and two ssODNs replace 58-kb of the rat Cyp2d cluster with a 6.2-kb human CYP2D6 gene. These ssODN-mediated KI protocols can be applied to any target site with any donor vector without the need to construct homology arms, thus simplifying genome engineering in living organisms. PMID:26786405

  8. The SsDREB Transcription Factor from the Succulent Halophyte Suaeda salsa Enhances Abiotic Stress Tolerance in Transgenic Tobacco

    Directory of Open Access Journals (Sweden)

    Xu Zhang

    2015-01-01

    Full Text Available Dehydration-responsive element-binding (DREB transcription factor (TF plays a key role for abiotic stress tolerance in plants. In this study, a novel cDNA encoding DREB transcription factor, designated SsDREB, was isolated from succulent halophyte Suaeda salsa. This protein was classified in the A-6 group of DREB subfamily based on multiple sequence alignments and phylogenetic characterization. Yeast one-hybrid assays showed that SsDREB protein specifically binds to the DRE sequence and could activate the expression of reporter genes in yeast, suggesting that the SsDREB protein was a CBF/DREB transcription factor. Real-time RT-PCR showed that SsDREB was significantly induced under salinity and drought stress. Overexpression of SsDREB cDNA in transgenic tobacco plants exhibited an improved salt and drought stress tolerance in comparison to the nontransformed controls. The transgenic plants revealed better growth, higher chlorophyll content, and net photosynthesis rate, as well as higher level of proline and soluble sugars. The semiquantitative PCR of transgenics showed higher expression of stress-responsive genes. These data suggest that the SsDREB transcription factor is involved in the regulation of salt stress tolerance in tobacco by the activation of different downstream gene expression.

  9. Microstructural studies of suck cast (Zr-SS)-3 and 5 AI alloys for nuclear metallic waste form

    International Nuclear Information System (INIS)

    Management of radioactive metallic waste using 'alloy melting route' is currently being investigated. For disposal of Zr and SS base nuclear metallic wastes, Zr-stainless steel (SS) hybrid alloys are being considered as baseline alloys for developing metallic-waste-form (MWF) alloys. In this context Zr-16 wt. %55 has been selected for MWF alloy in our previous study. In present study, to include amorphous phase in this alloy, 3 and 5 wt. % Al has been added in order to improve desirable properties and useful features of MWF and the two alloys have been prepared by suck casting techniques. Microstructure of these alloys have been investigated by optical and electron microscopy which shows occurrence of two different phases, e.g. dark grey and white phases, in (Zr-16 SS)-3 Al and three different phases, e.g. grey, dark grey and white phases in (Zr-16 SS)-5 AI. Electron diffraction and X-ray diffraction (XRD) analyses of these two alloy specimens revealed the occurrence of Zr (Fe, Cr, AI) (dark grey) and Zr2 (Fe, Cr, AI) (white) phases in (Zr-16 SS)-3 Al whereas, Zr (Fe, Cr, AI) (dark grey), Zr2 (Fe, Cr, AI) (grey) and Zr3(Fe, Cr, AI) (white) phases were found in (Zr-16 SS)-5 AI. In addition, presence of amorphous phase was indicated by XRD analysis that could be confirmed by transmission electron microscopy of these two alloys. (author)

  10. Differential sensitivity of the nicotinic receptor of long (LS) and short (SS) sleep mice to ethanol (E) and forane (F)

    Energy Technology Data Exchange (ETDEWEB)

    McArdle, J.J.; Choi, J.J. (New Jersey Medical School, Newark (USA))

    1989-02-09

    Studies of inbred mice indicate that heredity determines the behavioral response to CNS depressants. For example, LS mice lose their righting reflex at blood levels of E having no effect on this reflex of SS mice. In order to determine if such differential sensitivity extends to the effects of depressants known to alter the mean open time (tau) of the ion channel activated by the nicotinic acetylcholine receptor (AR), we used an extracellular electrode to record miniature end-plate currents (23 C) from the triangularis sterni muscle of adult male LS and SS mice. The average decay time constant (tau) of 70 currents was calculated before, during and after drug exposure. Tau was the same for LS and SS mice (1.41 {plus minus} 0.03 mS and 1.47 {plus minus} 0.02 mS, respectively) prior to treatment and was reversible prolonged by E and shortened by F as expected. However, tau of SS mice was more responsive. For example, 25 mM of E increased tau by 12.9% and 3.8% in SS and LS mice, respectively. Likewise, the decrease of tau in response to 3 mM F was 18.5% and 9.2%. The net result was that the curve relating tau for LS mice to drug concentration was to the right of the for SS mice. These data suggest that the sensitivity of the peripheral AR to CNS depressants can be genetically controlled.

  11. ssODN-mediated knock-in with CRISPR-Cas for large genomic regions in zygotes.

    Science.gov (United States)

    Yoshimi, Kazuto; Kunihiro, Yayoi; Kaneko, Takehito; Nagahora, Hitoshi; Voigt, Birger; Mashimo, Tomoji

    2016-01-01

    The CRISPR-Cas system is a powerful tool for generating genetically modified animals; however, targeted knock-in (KI) via homologous recombination remains difficult in zygotes. Here we show efficient gene KI in rats by combining CRISPR-Cas with single-stranded oligodeoxynucleotides (ssODNs). First, a 1-kb ssODN co-injected with guide RNA (gRNA) and Cas9 messenger RNA produce GFP-KI at the rat Thy1 locus. Then, two gRNAs with two 80-bp ssODNs direct efficient integration of a 5.5-kb CAG-GFP vector into the Rosa26 locus via ssODN-mediated end joining. This protocol also achieves KI of a 200-kb BAC containing the human SIRPA locus, concomitantly knocking out the rat Sirpa gene. Finally, three gRNAs and two ssODNs replace 58-kb of the rat Cyp2d cluster with a 6.2-kb human CYP2D6 gene. These ssODN-mediated KI protocols can be applied to any target site with any donor vector without the need to construct homology arms, thus simplifying genome engineering in living organisms. PMID:26786405

  12. A theoretical investigation on anomalous switching of single-stranded deoxyribonucleic acid (ssDNA) monolayers by water vapor

    Science.gov (United States)

    Zhao, Xin-Jun; Gao, Zhi-Fu; Jiang, Zhong-Ying

    2015-04-01

    In this paper, we use a molecular theory to study the anomalous switching of ssDNA monolayers. Here, both ssDNA-water and water-water hydrogen bonds and their explicit coupling to the ssDNA conformations are considered. We find that hydrogen bonding becomes a key element in inducing the anomalous switching of ssDNA monolayers. This finding accords well with the experimental observations. Based on our theoretical model, we predict that the anomalous switching induced by water vapor will be applicable to a wide range of hydrogen bonds polymers, and ssDNA-water hydrogen bonds and water-water hydrogen bonds hybridization will lead to the hydrogen-bond network formation of 3D ssDNA monolayers. Project supported by the National Natural Science Foundation of China (Grant Nos. 21264016, 11464047, and 21364016), the National Basic Research Program of China (Grant No. 2012CB821500), and the Natural Science Foundation of Xinjiang Uygur Autonomous Region, China (Grant No. 2013211A053).

  13. Thick SS316 materials TIG welding development activities towards advanced fusion reactor vacuum vessel applications

    Science.gov (United States)

    Kumar, B. Ramesh; Gangradey, R.

    2012-11-01

    Advanced fusion reactors like ITER and up coming Indian DEMO devices are having challenges in terms of their materials design and fabrication procedures. The operation of these devices is having various loads like structural, thermo-mechanical and neutron irradiation effects on major systems like vacuum vessel, divertor, magnets and blanket modules. The concept of double wall vacuum vessel (VV) is proposed in view of protecting of major reactor subsystems like super conducting magnets, diagnostic systems and other critical components from high energy 14 MeV neutrons generated from fusion plasma produced by D-T reactions. The double walled vacuum vessel is used in combination with pressurized water circulation and some special grade borated steel blocks to shield these high energy neutrons effectively. The fabrication of sub components in VV are mainly used with high thickness SS materials in range of 20 mm- 60 mm of various grades based on the required protocols. The structural components of double wall vacuum vessel uses various parts like shields, ribs, shells and diagnostic vacuum ports. These components are to be developed with various welding techniques like TIG welding, Narrow gap TIG welding, Laser welding, Hybrid TIG laser welding, Electron beam welding based on requirement. In the present paper the samples of 20 mm and 40 mm thick SS 316 materials are developed with TIG welding process and their mechanical properties characterization with Tensile, Bend tests and Impact tests are carried out. In addition Vickers hardness tests and microstructural properties of Base metal, Heat Affected Zone (HAZ) and Weld Zone are done. TIG welding application with high thick SS materials in connection with vacuum vessel requirements and involved criticalities towards welding process are highlighted.

  14. Long term high temperature oxidation resistance of a nanoceria coated 316 SS under dry air conditions

    International Nuclear Information System (INIS)

    In this work, nanoceria dip coated and uncoated 316 stainless steels were exposed to dry air at 1073–1273 K for times of up to 250 h. From this work, measured activation energies, Q = 256 kJ mol−1 and Q = 240 kJ mol−1 were found for coated and uncoated 316 SS, respectively. In the coated steel, the activation energy for oxidation cannot be attributed to a single mass transport mechanism. In addition, the scale morphologies, as well as the dominant oxide phases were determined by X-ray diffraction means. It was found that in the nanoceria dip coated steels, the scale was fine grained and highly adherent. Oxidation at increasing temperatures in the coated steels favored the development of the spinel ((Mn, Cr)3O4 structure and at 1273 K with the presence of Fe2O3 was severely hindered. In contrast, in the uncoated steel, a relatively thick scale, predominantly Fe2O3 developed and it exhibited severe damage through spallation and detachment from the steel substrate. Also, the resultant grain structure was rather coarse and it consisted of faceted grains with continuous nucleation/growth at grain ledges. - Highlights: • A 316 SS was dip coated with 3.45 nm nanoceria particles and oxidized in hot dry air. • Activation energies were determined by exposure to 1073–1273 K for up to 250 h. • The coated 316 SS exhibited a reduction in oxidation rates of 1–2 orders of magnitude. • At 1273 K the scale developed in the coated steel was a fine grained spinel MnCr2O4

  15. Tiimityöskentely Joensuun tulojen käsittelyn palveluryhmässä

    OpenAIRE

    Lötjönen, Mira

    2014-01-01

    Tämän opinnäytetyön tehtävä oli kartoittaa Joensuun tulojen käsittelyn palveluryhmään perustettujen tiimien ja tiimien jäsenten kokemukset tiimityöskentelystä, sekä perehtyä tiimityöskentelyyn liittyvään kirjallisuuteen. Palkeet on valtiovarainministeriön hallinnon alalla toimiva palvelukeskus, joka tuottaa kokonaisvaltaisesti talous- ja henkilöstöpalveluja valtionhallinnon virastoille, laitoksille ja rahastoille. Tulojen käsittelyn palveluryhmässä on vuoden 2014 alkupuolella siirrytty y...

  16. Kehityskeskustelu osaamisen johtamisen välineenä hoitotyössä

    OpenAIRE

    Laakso, Heli

    2010-01-01

    Tämän kehittämistyön tavoitteena oli kartoittaa nykyinen kehityskeskustelukäytäntö Kymenlaakson sairaalapalvelut Oy:ssä ja tehdä yritykselle suositus yhtenäisestä kehityskeskustelumallista osaamisen johtamisen näkökulmasta. Kokonaistavoitteeseen päästään selvittämällä nykytilanne Sairaalapalvelut Oy:n esimiehiltä. Kirjallisuuden sekä aikaisempien tutkimuksien avulla oli tarkoitus selvittää hyvä kehityskeskustelukäytäntö ja tehdä näistä kehityskeskustelusuositus. Aineistoa analysoitiin sisäl...

  17. Helsingin matkailullisen brändi-identiteetin vahvistuminen Helsinki-oppaan työssä

    OpenAIRE

    Riehunkangas, Juha; Oikarinen, Satu

    2010-01-01

    Tämä opinnäytetyö käsittelee Helsingin tavoittelemaa matkailubrändiä ja brändi-identiteetin vahvistumista Helsinki-oppaan työssä. Helsingin matkailustrategian 2009–2012 mukaan Helsinki tavoittelee vahvaa matkailubrändiä. Tutkimuksen tavoitteena oli selvittää, ovatko Helsinki-oppaiden tärkeinä pitämät ja korostamat vetovoimatekijät linjassa strategiassa määriteltyjen brändi-elementtien kanssa. Toisena tavoitteena oli selvittää Helsinki-oppaiden kiinnostus lisäkoulutukseen ja koulutusaiheisiin....

  18. Turkkilaisen kulttuurin huomioiminen perehdyttäessä suomalaisessa työyhteisössä

    OpenAIRE

    Sargin, Meliha

    2014-01-01

    Tämän opinnäytetyön tavoitteena oli selvittää turkkilaisten työntekijöiden kohtaamia haasteita, kun heidät perehdytetään suomalaisessa työyhteisössä. Opinnäytetyön ta-voitteena oli olla selkeä, ytimekäs ja helppolukuinen. Perehdytysprosessissa eri kulttuuritaustat asettavat haasteita neuvon antamiseen, sekä nopeaan ja tehokkaaseen työtehtäviin perehdyttämiseen. Työhön opastettaessa tarvi-taan laajempaa taustatietoa kulttuurista ja uudenlaista ajattelua totuttuihin toimintata-poihin verrat...

  19. $b\\to ss{\\bar d} $ decay in Randall-Sundrum models

    CERN Document Server

    Lü, Cai-Dian; Qin, Qin

    2016-01-01

    The extremely small branching ratio of $b\\to ss{\\bar d}$ decay in the Standard Model makes it a suitable channel to explore new physics through various extensions of the Standard Model. We study this $\\Delta S=2$ process in Randall-Sundrum models, including the custodially protected and the bulk-Higgs Randall-Sundrum models. Exploring the parameter space of these models constrained by $K^0-\\bar K^0$ mixing and $B_s^0-\\bar B_s^0$ mixing, it suggests a possible accessibility of this decay in future experiments.

  20. Purification and preliminary characterization of a xylanase from Thermomyces lanuginosus strain SS-8

    OpenAIRE

    Shrivastava, Smriti; Shukla, Pratyoosh; Mukhopadhyay, Kunal

    2011-01-01

    Thermomyces lanuginosus SS-8 was isolated from soil samples that had been collected from near self-heating plant material and its extracellular cellulase-free xylanase purified approximately 160-fold using ion exchange chromatography and continuous elution electrophoresis. This xylanase was thermoactive (optimum temperature 60 °C) at pH 6.0 and had a molecular weight of 23.79 kDa as indicated by SDS-PAGE electrophoresis. The xylanase rapidly hydrolyzed xylan directly to xylose without the pro...

  1. Hoitotyön arvot käytännössä

    OpenAIRE

    Mankinen, Hanna; Lohilahti, Jenna

    2009-01-01

    Kainuussa on hoitotyöntekijöille vuonna 2007 jaettu Hoitotyön arvokirja. Kirjaan Kainuun maakunta -kuntayhtymä on määritellyt viisi hoitotyön arvoa suuntaviivoiksi kainuulaiselle hoitotyölle. Näitä arvoja ovat ihmisarvon kunnioittaminen, huolenpito ja lähimmäisenrakkaus, terveys, oikeudenmukaisuus ja kehitysmyönteisyys. Käsittelemme opinnäytetyössämme sairaanhoitajien toteuttamaa potilasohjausta Hoitotyön arvokirjan näkökul-masta. Toimeksiantajana toimi Kainuun maakunta -kuntayhtymä. Opin...

  2. Asiakkaan osallistumisen ja suoriutumisen arviointi Hoitokoti Kuikanpesässä

    OpenAIRE

    Härkönen, Matti; Hirvonen, Kati

    2012-01-01

    Opinnäytetyön toimeksiantaja ja yhteistyökumppani oli Ilomantsissa toimiva Hoitokoti Kuikanpesä, joka tarjoaa palveluasumista sekä tehostettua palveluasumista mielenterveys- ja mielenterveyspäihdekuntoutujille. Hoitokoti on aloittanut toimintansa keväällä 2011. Opinnäytetyön tavoitteena oli löytää luotettava ja työelämässä psykiatristen asiakkaiden kanssa käyttökelpoiseksi havaittu toimintaterapian arviointikokonaisuus hoitokodin toimintatera- peutin käyttöön. Tarkoituksena oli, että opinn...

  3. VPN virtuaalikoneiden etäkäytössä

    OpenAIRE

    Mustonen, Marko

    2014-01-01

    Opinnäytetyön aiheena oli virtuaalikoneiden etäkäytön mahdollistavan VPN-ratkaisun luominen Oulun Ammattikorkeakoululle. Tavoitteena oli korvata aiemmin käytössä ollut VPN-ratkaisu nykyaikaisella ratkaisulla. Opinnäytetyön tietoperusta koostui lähinnä tietoverkkoihin ja VPN-ratkaisuihin liittyvistä asioista. Tietoperusta kattoi esimerkiksi seuraavat käsitteet: IP-osoitteet (Internet Protocol), Dynamic Host Configuration Protocol (DHCP), Domain Name System (DNS), osoitteenmuunnos (NAT), ak...

  4. Microstructures of an Ultrafine Grained SS400 Steel in an Industrial Scale

    Institute of Scientific and Technical Information of China (English)

    2006-01-01

    The microstructures of a SS400 steel after thermomechanical control process (TMCP) in an industrial production were observed by optical microscope, scanning electron microscope (SEM) and transmission electron microscope (TEM). The results indicated that the size of ferrite grains was 4~5μm, and the volume fraction of ferrite was around 70%. The types of the ultrafine ferrite grains were analyzed and the strengthening mechanisms were discussed. The results show that the ultrafine ferrite grains came from three processes, i.e.deformation induced ferrite transformation (DIFT), dynamic recrystallization of ferrite and accelerated cooling process. The increase in the strength of the material was mainly due to the grain refining.

  5. Valmentamisen mahdollisuudet työelämässä

    OpenAIRE

    Sarpo, Joni

    2012-01-01

    Tämän opinnäytetyön tarkoituksena oli tutkia, millaisia mahdollisuuksia valmentamisella on työelämässä. Päätavoitteena oli saada esille erilaisten ihmisten käsityksiä ja kokemuksia valmentamisesta ja sen hyödyistä. Aihe oli erittäin ajankohtainen, sillä uusi sukupolvi on astumassa työelämään ja sen odotukset työelämältä ja johtamiselta sisältävät samoja asioita kuin valmentaminen. Tutkimus toteutettiin laadullisena ja aineisto kerättiin teemahaastatteluilla. Haastattelut purettiin narrati...

  6. Corrosion behaviour and mechanical property changes of AISI 316 SS exposed in liquid sodium environment

    International Nuclear Information System (INIS)

    Under the synergistic influence of high temperature and sodium, different forms of corrosion and mass transfer may occur in the SS components which will have an adverse influence on the mechanical properties. Samples from different sections of the mass transfer loop constructed for this purpose were taken for the present analysis. The purity of the sodium during the course of the experiment was controlled by continuously cold-trapping a part of the sodium. The paper analyses the chemical and mechanical changes and discusses the effect of sodium-exposure from thermal-ageing

  7. Neutronics and activation analysis of a SS316 based experimental D-T fusion power reactor

    International Nuclear Information System (INIS)

    Neutronics and activation analysis for a SS316 based experimental tokamak fusion power reactor was completed using a two-dimensional neutronics model because of its practical features. Operating and decay power densities in all reactor components were obtained. The significant increase of neutron fluxes in the vacuum duct region was revealed. Maintenance and waste management aspects of the reactor components were investigated. Impacts on these aspects due to important minor alloying elements and impurities such as Mn, Co, Mo, and Nb were addressed

  8. Miten laki ammatillisesta aikuiskoulutuksesta käytännössä toteutuu

    OpenAIRE

    Salo, Raija

    2011-01-01

    Opinnäytetyöni aiheena on miten Laki ammatillisesta aikuiskoulutuksesta käytännössä toteutuu. Tutkimus on rajattu käsittelemään vain tutkintotavoitteista aikuisten ammatillista koulutusta. Työlläni haluan tuoda esille ammatillista aikuiskoulutusta ohjaavaa lainsäädäntöä ja sen toteutumista. Tutkimukseni on teorialähtöinen ja tarkastelen ongelmaa aikuiskoulutusorganisaatioiden näkökulmasta. Työni on laadullinen eli kvalitatiivinen tutkimus. Aikuisten ammatillisessa koulutuksessa opetus- ja...

  9. Maailma kylässä 2010 –festivaalin yhteistyökumppanitutkimus

    OpenAIRE

    Lappalainen, Tomi

    2011-01-01

    Maailma kylässä –festivaali on toukokuun lopussa Helsingissä järjestettävä kaksipäiväinen ilmaistapahtuma, joka esittelee eri kulttuureja ja niiden tarjontaa. Tapahtuma järjestettiin tänä vuonna yhdettätoista kertaa ja sen tapahtumapaikkoina toimivat Kaisaniemen puisto sekä Rautatientori. Tapahtuma tarjoaa tietoa monikulttuurisuudesta, kehitysyhteistyöstä ja globaaliasioista sekä mahdollisuuksista vaikuttaa asioihin omassa arjessaan. Näytteilleasettajina festivaalilla esillä ovat järjestöt, y...

  10. Role of ethanol on growth, laccase production and protease activity in Pycnoporus cinnabarinus ss3

    OpenAIRE

    Meza, Juan Carlos; Auria, Richard; Lomascolo, A.; Sigoillot, J.C.; Casalot, Laurence

    2007-01-01

    Laccase production by the strain Pycnoporus cinnabarinus ss3 was studied in a solid-state culture on sugar-cane bagasse using chemical compounds as inducers (ethanol, methanol, veratryl alcohol and ferulic acid). Laccase productions were about 5- to 8.5-fold higher than non-induced cultures. Liquid-culture experiments with "Glabeled ethanol were conducted. Ninety-eight percent of the initial amount of C-14 from ethanol was recovered as (CO2)-C-14, C-14-biomass and soluble C-14-compounds (main...

  11. Musapaja mielenterveyskuntoutuksessa : Musiikillisen toimintamallin kehittäminen vertaisryhmässä

    OpenAIRE

    Happonen, Juha

    2016-01-01

    Opinnäytetyössä kehitettiin Takuulla Yhdessä! –hankkeessa uutta toimintamallia Kuopion Psykiatrian Keskuksen mielenterveyskuntoutujien avohuollon musiikkipainotteiseen vertaisryhmätoimintaan. Toiminta oli suunnattu nuorille aikuisille KPK:n asiakkaille, jotka olivat kiinnostuneita musiikkiryhmän toiminnasta osana KPK:n tarjoamaa hoitoa. KPK:n piirissä tällaiselle toiminnalle oli ollut kysyntää jo pidemmän aikaa ja Takuulla Yhdessä! –hanke vastasi tähän tarpeeseen hankkeen tavoitteista käsin, ...

  12. Globaali tiedostaminen ja sen kehittymisen tukeminen : Intia-aiheiset teemaillat nuorisotyössä

    OpenAIRE

    Majamäki, Katri

    2015-01-01

    Majamäki, Katri. Globaali tiedostaminen ja sen kehittymisen tukeminen, Intia-aiheiset teemaillat nuorisotyössä. Diak, Pori, kevät 2015, 62 s., 8 liitettä. Diakonia – ammattikorkeakoulu, Sosiaalialan koulutusohjelma, sosionomi (AMK). Opinnäytetyön tavoitteena oli tarkastella nuorisotyön roolia nuorten tukemisessa globaaleja asioita tiedostaviksi yksilöiksi. Opinnäytetyön toiminnallisena osuutena suunniteltiin ja toteutettiin kaksi Intia-aiheista teemailtaa nuorille. Lisäksi osana työtä on ...

  13. Kehitysvammainen lapsi hoitotyössä - opas hoitotyön opiskelijoille

    OpenAIRE

    Riihimäki, Hanna

    2014-01-01

    Tämän opinnäytetyön aihealueena oli kehitysvammaisuus ja erityisesti kehitysvammaiset lapset hoitotyön opiskelijoiden näkökulmasta. Opinnäytetyön tarkoituksena oli laatia opas kehitysvammaisista lapsista hoitotyön opiskelijoille palvelukoti Pihlaja-Vaahteraan. Opinnäytetyön tavoitteena oli lisätä hoitotyön opiskelijoiden tietämystä kehitysvammaisuudesta, sekä parantaa heidän valmiuksiaan kohdata kehitysvammainen lapsi. Opas tehtiin yhteistyössä Satakunnan sairaanhoitopiirin organisaatioon kuu...

  14. Psykiatrisen sairaanhoitajan valmiudet ja kokemukset kuuron potilaan hoitotyössä

    OpenAIRE

    Siljanen, Kaisa

    2012-01-01

    Opinnäytetyön tutkimuksen tarkoituksena oli kuvata psykiatristen sairaanhoitajien valmiuksia kuuron potilaan kohtaamiseen. Tarkoituksena oli myös selvittää psykiatristen sairaanhoitajien kokemuksia kuuron potilaan hoitotyöstä. Opinnäytetyön tavoitteena oli saada lisää tietoa ja siten kehittää kuuron potilaan psykiatrista hoitotyötä. Sairaanhoitajat voivat hyödyntää tutkimuksen tuloksia kohdatessaan kuuron potilaan niin psykiatrisessa kuin somaattisessakin hoitotyössä. Kuurot ovat viittoma...

  15. An evaluation of microbial growth and corrosion of 316L SS in glycol/seawater mixtures

    Science.gov (United States)

    Lee, Jason S.; Ray, Richard I.; Lowe, Kristine L.; Jones-Meehan, Joanne; Little, Brenda J.

    2003-01-01

    Glycol/seawater mixtures containing > 50% glycol inhibit corrosion of 316L stainless steel and do not support bacterial growth. The results indicate bacteria are able to use low concentrations of glycol (10%) as a growth medium, but bacterial growth decreased with increasing glycol concentration. Pitting potential, determined by anodic polarization, was used to evaluate susceptibility of 316L SS to corrosion in seawater-contaminated glycol. Mixture containing a minimum concentration of 50% propylene glycol-based coolant inhibited pitting corrosion. A slightly higher minimum concentration (55%) was needed for corrosion protection in ethylene glycol mixtures.

  16. "Kun on hyvässä kunnossa jaksaa paljon paremmin" : kuntosaliopas CP-vammaisille

    OpenAIRE

    Hellsten, Johanna; Teerikoski, Mari

    2013-01-01

    Opinnäytetyön tarkoituksena oli suunnitella kuntosaliopas lievästi CP-vammaisille. Kuntosaliopas toteutettiin yhteistyössä Suomen CP-liitto ry:n kanssa. CP-vamma on elinikäinen vamma, jonka aiheuttaa varhaislapsuudessa saatu aivovaurio tai aivojen kehityshäiriö. CP-vammaisten toimintakyky on hyvin vaihteleva ja se alkaa heiketä jo nuorena. Tarve kuntosalioppaaseen tuli Suomen CP-liitto ry:ltä. CP-vammaiselle suunnattua kuntosaliopasta ei ole aiemmin suunniteltu. Oppaan laatimista ohjasi kirja...

  17. Tieto- ja viestintäteknologia autokaupan toimintaympäristössä

    OpenAIRE

    Hartikainen, Teemu

    2013-01-01

    Opinnäytetyön tarkoituksena on tutkia, millaisia käsityksiä ja kokemuksia automyyjillä on tieto- ja viestintäteknologian merkityksestä ja muutoksesta heidän työssään. Tutkielmassa selvitetään, miten tieto- ja viestintäteknologian kehitys on muuttanut automyynnin käytänteitä. Tavoitteena on hyödyntää tutkielman tuloksia ensisijaisesti kohdeyrityksessä. Opinnäytetyö on toteutettu laadullisena haastattelututkimuksena. Aineisto koostuu keskisuuren autoliikeketjun kuuden automyyjän teemah...

  18. Asiakaspalvelusta ylivoimatekijä esimiestyöllä : Case Osuuskauppa PeeÄssä

    OpenAIRE

    Makkonen, Reetta; Vallioniemi, Sarianne

    2013-01-01

    Asiakaspalvelu ja sen laatu ovat tänä päivänä yrityksen tärkeimpiä kilpailukeinoja. Jatkuvasti etsitään tapoja erottua kilpailijoista, ja siihen on alettu kiinnittämään erityisen paljon huomiota. Asiakaspalvelun laadun kehittäminen on jatkuva prosessi, johon vaaditaan tavoitteellista laatu- ja muutosjohtamista sekä henkilöstön sitoutumista. Tämän opinnäytetyön toimeksianto on tullut Osuuskauppa PeeÄssän johdolta ja tutkimuksen tarkoituksena on sel-vittää kuinka esimiestyöllä voidaan vaik...

  19. Mobiilitietoturvan kehittäminen Halti Oy:ssä ohjeiston avulla

    OpenAIRE

    Hyyryläinen, Olli

    2011-01-01

    Mobiililaitteista älypuhelimien ja sormitietokoneiden määrä on kasvanut räjähdysmäisesti viimeisen ‎viiden vuoden aikana. Perinteisten matkapuhelintenkin ominaisuudet ovat kehittyneet siihen ‎pisteeseen, että matkapuhelimia on jo vaikeaa luokitella niihin, jotka ovat tai eivät ole ‎älypuhelimia. Yrityksissä kehitys on huomattu, ja entistä useammalla työntekijällä on käytössään ‎älypuhelin laajakaistanopeuteen kykeneväksi mainostetulla langattomalla tiedonsiirtoyhteydellä. ‎Internet on työntek...

  20. GSM-signaalin tasomittaukset sairaalaympäristössä ja parannusehdotukset

    OpenAIRE

    Torvinen, Jyri-Jussi

    2013-01-01

    Työn aiheena oli suorittaa TeliaSoneran GSM-verkon kenttämittauksia sairaa-laympäristössä ja tehdä saatujen tuloksien perusteella parannusehdotuksia. Nykypäivänä operaattorien panostus GSM-verkon toimivuuteen on vähentynyt, vaikkakin GSM toimii lähes koko maa kattava perusverkkona suurelle osalle suomalaisia. Työ edellytti GSM-verkossa tapahtuvien mittausten ja niihin käytettävien mittalaitteiden tuntemusta. Työ toteutettiin Oulussa Pohjois-Pohjanmaan sairaanhoitopiirin OYS:n lasten-saira...

  1. Psykososiaalinen tuki ja monikulttuurinen ohjaus- ja neuvontatyö nuorten maahanmuuttajien opetusryhmässä

    OpenAIRE

    Paunila, Vaula

    2014-01-01

    TIIVISTELMÄ Paunila, Vaula. Psykososiaalinen tuki ja monikulttuurinen ohjaus- ja neuvontatyö nuorten maahanmuuttajien opetusryhmässä. Opinnäytetyö, syksy 2014, 138 s, 3 liitettä. Diakonia-ammattikorkeakoulu, Sosiaalialan koulutusohjelma, sosionomi (AMK). Kehittämispainotteisen opinnäytetyön lähtökohtana oli tehdä näkyväksi Espoossa kehitetty monialainen toimintamalli oppivelvollisuusiän ylittäneiden maahanmuuttajanuorten kotoutumisen edistämisessä. Opinnäytetyön tarkoituksena oli k...

  2. Hoitotyöntekijöiden kokemuksia palliatiivisen potilaan hoitotyössä

    OpenAIRE

    Haikola, Kati

    2008-01-01

    Tämän opinnäytetyön tarkoituksena on kuvata, miten hoitotyöntekijät kokevat palliatiivisen potilaan hoitotyön. Opinnäytetyössämme kuvataan myös hoitotyön tekijöiden jaksamista ja palliatiivisen potilaan hoitotyön laatua. Tutkimuksemme on kvalitatiivinen ja avoimella kyselylomakkeella tehty tutkimus. Aineisto kerättiin erään palliatiivisen osaston hoitohenkilökunnalta. Vastauksia saatiin kymmenen kappaletta. Saatu aineisto analysoitiin induktiivisella sisällönanalyysilla. Tutkimustuloste...

  3. Aggressiivisesti käyttäytyvän potilaan kohtaaminen hoitotyössä

    OpenAIRE

    Andersen, Asta

    2015-01-01

    Opinnäytetyö toteutettiin Oulun kaupunginsairaalan akuuttivuodeosastolla. Metodologisena lähestymistapana oli toimintatutkimus, jonka ensimmäisen syklin tarkoituksena oli kuvata hoitotyöntekijöiden (hoitajien) kokemuksia uhka- ja väkivaltatilanteista. Tutkimustehtäväksi muodostui, minkälaisia uhka- ja väkivaltatilanteita hoitajat ovat kokeneet hoitotyössä? Tavoitteena oli kehittää hoitajien valmiuksia kohdata aggressiivinen potilas yhteisten pelisääntöjen ja vuorovaikutusosaamisen avulla. Tut...

  4. Hoitohenkilökunnan kokemuksia rumpumusiikin vaikutuksesta muistisairaan potilaan kuntouttavassa hoitotyössä

    OpenAIRE

    Ilonen, Hanna

    2016-01-01

    Opinnäytetyön tarkoituksena oli selvittää muistisairaan potilaan kuntouttavaan hoitotyöhön osallistuvien hoitajien ja hoitohenkilökunnan kokemuksia rummun käytöstä musiikin välineenä hoitotyössä psykogeriatrisella osastolla Pirkanmaan sairaanhoitopiirissä. Opinnäytetyön tehtävinä oli selvittää hoitohenkilökunnan kokemuksia siitä, miten musiikin käyttö vaikuttaa psykogeriatrisessa osastohoidossa oleviin muistisairaisiin potilaisiin, minkälainen työväline musiikki on osana kuntouttavaa hoitotyö...

  5. An Integrated SS-VSA Analysis of Changing Job Roles

    OpenAIRE

    Jim Spohrer; Gaetano M. Golinelli; Paolo Piciocchi; Clara Bassano

    2010-01-01

    This paper presents a first attempt at an integrated Service Science (SS) and Viable Systems Approach (VSA) analysis of the real-world phenomenon of changing jobs roles. Changing job roles is important to quality of life and yet understudied by systems scientists. Today, individuals changing jobs multiple times during their working life is the norm. The average person born in the later years of the US baby boom held 10.8 jobs from age 18 to age 42 (BLS [BLS 2008. Number of jobs held, labor ma...

  6. Vihreiden arvojen merkitys automarkkinoinnissa showroom-ympäristössä

    OpenAIRE

    Laurinus, Heikki

    2013-01-01

    Tämän opinnäytetyön tavoitteena oli tutkia ympäristöarvojen merkitystä automarkkinoinnissa showroom–ympäristössä automaahantuojien näkökulmasta. Tutkimus toteutettiin toimeksiantona Troi Helsinki Oy:lle. Troi Helsinki Oy on markkinointitoimisto, joka on erikoistunut tapahtumamarkkinointiin sekä muun muassa erilaisten näyttelytilojen suunnitteluun ja toteutukseen. Ympäristöarvot, yhteiskunnan kaikilla osa-alueilla, ovat erityisesti parin viime vuosikymmenen aikana olleet kasvava puheen- se...

  7. Application of nanoindentation for investigation of radiation damage in SS316 stainless steel

    International Nuclear Information System (INIS)

    The possibility of application of nanoindentation technique for investigation of mechanical properties (hardness and modulus of elasticity) of SS316 steel irradiated with 1.4 MeV Ar+ ions to a dose of 1·1017 cm-2 at 900 K has been considered. Methods of experimental data processing for extracting of physical characteristics of materials, in particular, hardening at irradiation to 25 dpa are analyzed. The influence of different factors on hardening is discussed involving results of transmission and scanning electron microscopy

  8. Guanxi-verkostojen merkitys kiinalaisessa liiketoimintaympäristössä

    OpenAIRE

    Hirvonen, Anne; Matveinen, Tarja

    2009-01-01

    Kiina muuttuu valtavaa vauhtia. Entinen suljettu valtio on kasvanut jättimahdiksi ja yhtäjak-soinen talouskasvu on jatkunut yhdeksän prosentin vuositasolla vuodesta 1979 lähtien. Ulko-maisia yrityksiä kiinnostavat Kiinan halvat työvoimakustannukset ja valtavat markkinat, mutta kiinalaiseen liiketoimintaympäristöön sopeutuminen vaatii yrityksiltä aikaa, kärsivällisyyttä ja oikeanlaisia suhteita. Vaikka ulkoiset muutokset ovat tapahtuneet häkellyttävän nopeasti, vaikuttavat ihmisten sisimmässä ...

  9. Elektronisen tanssimusiikin tuotanto : Tuotantotekniikka teoriassa ja käytännössä

    OpenAIRE

    Kaapro, Ville

    2010-01-01

    Opinnäytetyöni käsittelee elektronisen tanssimusiikin tuotantoa, työvaiheita ja tuotannossa käytettäviä työvälineitä. Käsittelen aihetta teoreettisesta näkökulmasta käyttäen apuna kirjallista lähdeaineistoa sekä omaa kokemusta ja taustatietoa aiheeseen liittyen. Opinnäytetyöni tavoitteena on antaa kokonaiskuva elektronisen tanssimusiikin tuottamisen työvaiheista ja tyypillisistä tuotantomenetelmistä esimerkkien kautta. Olen jakanut aihealueen työvaiheisiin, joita käsittelen selkeässä j...

  10. Synthesis and antioxidant evaluation of (S,S)- and (R,R)-secoisolariciresinol diglucosides (SDGs)

    OpenAIRE

    Mishra, Om P; Simmons, Nicholas; Tyagi, Sonia; Pietrofesa, Ralph; Shuvaev, Vladimir V.; Valiulin, Roman A.; Heretsch, Philipp; Nicolaou, K.C.; Christofidou-Solomidou, Melpo

    2013-01-01

    Secoisolariciresinol diglucosides (SDGs) (S,S)-SDG-1 (major isomer in flaxseed) and (R,R)-SDG-2 (minor isomer in flaxseed) were synthesized from vanillin via secoisolariciresinol (6) and glucosyl donor 7 through a concise route that involved chromatographic separation of diastereomeric diglucoside derivatives (S,S)-8 and (R,R)-9. Synthetic (S,S)-SDG-1 and (R,R)-SDG-2 exhibited potent antioxidant properties (EC50 = 292.17 ± 27.71 μM and 331.94 ± 21.21 μM, respectively) which compared well with...

  11. Hengellisen väkivallan tunnistaminen sosiaali- ja terveysalan työssä

    OpenAIRE

    Kuokkanen, Elina

    2011-01-01

    Opinnäytetyöni aihe on hengellinen väkivalta ja sen tunnistaminen sosiaali- ja terveysalan työssä. Sosiaalialan työhön keskeisesti kuuluu väkivallan tunnistaminen, kohtaaminen ja ehkäiseminen. Hengellinen väkivalta erotetaan nykyisin usein omaksi väkivallan lajikseen, mutta siitä huolimatta se on aiheena melko tuntematon ja vähän tutkittu. Työni tavoitteena oli lisätä sosiaali- ja terveysalan ammattilaisten tietämystä aiheesta ja mahdollisuuksia aiheen käsittelyyn asiakastilanteis...

  12. Rahtikirjatoimintojen kehittäminen ERP-järjestelmässä

    OpenAIRE

    Aaltonen, Heikki

    2009-01-01

    Tämän insinöörityön tarkoituksena oli kehittää Inlook Oy:n toiminnanohjausjärjestelmään liiketoiminnan kannalta toimivat rahtikirjatoiminnot. Rahtikirjatoimintojen kehitystyö tehtiin pääosin Inlook Oy:n Herttoniemen teollisuusmaalaamon tarpeita silmällä pitäen. Rahtikirjatoimintojen määrittelyt tehtiin yhdessä teollisuusmaalaamon henkilökunnan kanssa. Työssä määriteltiin Inlookin liiketoiminnan kannalta tarpeelliset rahtikirjatoiminnot ja ominaisuudet toiminnanohjausjärjestelmän ohjelmisto...

  13. IT-palvelun kehittäminen Veljekset Keskinen Oy:ssä

    OpenAIRE

    Hautamäki, Satu

    2013-01-01

    Opinnäytetyön toimeksiantajana toimi Veljekset Keskinen Oy, jonka IT-palvelu oli tarkastelun kohteena. Tavoitteena oli kehittää IT-palvelua ITIL-viitekehyksen oppien mukaan. Tarkoituksena oli parantaa tapahtumanhallinta- ja palvelupyyntöprosesseja sekä palvelupistetoimintaa ottamalla käyttöön palvelupyyntöjärjestelmä. Tapahtumanhallinta- ja palvelupyyntöprosessi määriteltiin ja kuvattiin ITIL-viitekehyksen mallin mukaan. IT-osastolla ei ollut käytössään ennestään mitään järjestelmää. Uuden jä...

  14. Toimiva internetmarkkinointi Lämpönen Oy:ssä

    OpenAIRE

    Karjula, Patricia

    2014-01-01

    Opinnäytetyössä luotiin toimiva internetmarkkinointisuunnitelma Lämpönen Oy:lle. Internetmarkkinointisuunnitelman tarkoituksena oli parantaa löydettävyyttä internetistä ja lisätä asiakasvirtaa, sekä viihtyvyyttä yrityksen kotisivuilla. Toiminnallisena osana tehtiin markkinointitoimenpide lämpöpumppujen myynnistä loma-asuntoihin. Lämpönen Oy on valtakunnallinen lämpöpumppujen myyntiin keskittynyt yritys, joka tarjoaa markkinoiden par-haat laitteet ja palvelut asennuksesta huoltoon. Lämpöse...

  15. Työssäoppimisen kehittäminen palvelumuotoilun avulla

    OpenAIRE

    Heikkilä, Toivo; Jauhiainen, Kirsi

    2012-01-01

    Ravintola-ala on hyvin suhdanneherkkää. Taantumat ja noususuhdanteet vaikuttavat suoraan palveluiden käyttöasteeseen. Työtehot ovat kovat ja jatkuvasti nousussa. Ravintolaliiketoiminta kaipaa uusia työntekijöitä ja oppilaitosyhteistyö on tärkeä väylä rekrytoinnin helpottamiseksi. Kilpailu asiakkaista on kovaa ja uusia yrittäjiä tulee alalle jatkuvasti. Vastaavasti joidenkin ravintoloiden elinkaari on lyhyt. Tämän kehittämistyön tavoitteena on kehittää työssäoppimista palveluliiketoiminnan...

  16. Myynnin haasteet kauneudenhoitoalan B2B-myyntityössä

    OpenAIRE

    Pulkkinen, Rosa

    2016-01-01

    Opinnäytetyön tavoitteena oli selvittää, mitä haasteita myyntiedustajat kohtaavat business- to-business-myyntityössään kauneudenhoitoalan organisaatiossa. Tutkimuksessa selvitettiin yhteistyöyrityksen myyntihenkilöstön työtyytyväisyys yleisellä tasolla. Tutkimuksen yhteistyökumppanina toimi suomalainen kauneudenhoitoalan yritys, joka myy ja markkinoi kauneus- ja hyvinvointituotteita alan ammattilaisille ja kuluttajille. Työn teoriaosuudessa käsiteltiin myyntiedustajan ammattia, työnkuvaa,...

  17. SS, panadería y pastelería gourmet

    OpenAIRE

    Bernal Rodríguez, Érica; Forero Perilla, María Paula

    2013-01-01

    El objetivo general, es realizar un plan de negocios para crear la empresa Süss, en la ciudad de Bogotá, elaborando un estudio de mercado, el cual nos proporcione información suficiente sobre la competencia, sobre los precios y los gustos de los clientes potenciales. Lo anterior además de proporcionar la información anteriormente nombrada, permitirá hacer el plan de mercadeo donde se definirán: el concepto del producto, las estrategias de distribución, precio , plaza y promoción.

  18. Restonomiopiskelijoiden työssä käyminen ja sen vaikutus opintoihin

    OpenAIRE

    Virtanen, Sanna-Mari

    2011-01-01

    Opinnäytetyön aiheena oli restonomiopiskelijoiden työssä käyminen ja sen vaikutus opintoihin. Aihe on ajankohtainen, sillä etenkin matkailu- ja ravitsemisalalla työnteko opintojen ohella on vuosi vuodelta yleistynyt. Monella opiskelijalla ei ole vaihtoehtoja, vaan töitä on tehtävä, jos haluaa säilyttää edes jonkinlaisen elintason. Opinnäytetyön tuottamista uusista tiedoista hyötyivät Saimaan ammattikorkeakoulu ja sen opettajat ja opiskelijat. Työntekoa ja sen vaikutuksia opiskeluun tutkit...

  19. Evidence for 4-chlorobenzoic acid dehalogenation mediated by plasmids related to pSS50. [Alcaligenes

    Energy Technology Data Exchange (ETDEWEB)

    Layton, A.C.; Sanseverino, J.; Wallace, W.; Corcoran, C.; Sayler, G.S. (Univ. of Tennessee, Knoxville (United States))

    1992-01-01

    The potential for polychlorinated biphenyl biodegradation is of environmental interest because of the toxicity and bioaccumulation of these compounds. Biodegradation studies on 4-chlorobiphenyl (4CB), a model for polychlorinated biphenyl degradation, have focused mainly on the genes and enzymes that permit the degradation of 4CB to 4-chlorobenzoate (4CBA). Most biphenyl- and polychlorinated biphenyl-degrading strains accumulate the corresponding chlorobenzoic acids without degrading the chlorobenzoate to CO{sub 2}. The study demonstrated that the 4-chlorobiphenyl-degrading Alcaligenes sp. strain ALP83 can degrade 4-chlorobenzoate to 4-hydroxybenzoate. The dehalogenase activity is correlated with a 10-kb fragment carried on plasmid pSS70.

  20. Effects of co-habitation between Anopheles gambiae s.s. and Culex quinquefasciatus aquatic stages on life history traits

    Directory of Open Access Journals (Sweden)

    Kweka Eliningaya J

    2012-02-01

    Full Text Available Abstract Background The effective measures for the control of malaria and filariasis vectors can be achieved by targeting immature stages of anopheline and culicine mosquitoes in productive habitat. To design this strategy, the mechanisms (like biotic interactions with conspecifc and heterospecific larvae regulating mosquito aquatic stages survivorship, development time and the size of emerging adults should be understood. This study explored the effect of co-habitation between An. gambiae s.s. and Cx. quinquefasciatus on different life history traits of both species under different densities and constant food supply in the habitats of the same size under semi-natural conditions. Methods Experiments were set up with three combinations; Cx. quinquefasciatus alone (single species treatment, An. gambiae s.s. alone (single species treatment; and An. gambiae s.s. with Cx. quiquefasciatus (co-habitation treatment in different densities in semi field situation. Results The effect of co-habitation of An. gambiae s.s. and Cx. quinquefasciatus was found to principally affect three parameters. The wing-lengths (a proxy measure of body size of An. gambiae s.s. in co-habitation treatments were significantly shorter in both females and males than in An. gambiae s.s single species treatments. In Cx. quinquefasciatus, no significant differences in wing-length were observed between the single species and co-habitation treatments. Daily survival rates were not significantly different between co-habitation and single species treatments for both An. gambiae s.s. and Cx. quinquefasciatus. Developmental time was found to be significantly different with single species treatments developing better than co-habitation treatments. Sex ratio was found to be significantly different from the proportion of 0.5 among single and co-habitation treatments species at different densities. Single species treatments had more males than females emerging while in co

  1. The Borrelia burgdorferi telomere resolvase, ResT, anneals ssDNA complexed with its cognate ssDNA-binding protein.

    Science.gov (United States)

    Huang, Shu Hui; Kobryn, Kerri

    2016-06-20

    Spirochetes of the genus Borrelia possess unusual genomes that consist in a linear chromosome and multiple linear and circular plasmids. The linear replicons are terminated by covalently closed hairpin ends, referred to as hairpin telomeres. The hairpin telomeres represent a simple solution to the end-replication problem. Deoxyribonucleic acid replication initiates internally and proceeds bidirectionally toward the hairpin telomeres. The telomere resolvase, ResT, forms the hairpin telomeres from replicated telomere intermediates in a reaction with similarities to those promoted by type IB topoisomerases and tyrosine recombinases. ResT has also been shown to possess DNA single-strand annealing activity. We report here that ResT promotes single-strand annealing of both free DNA strands and ssDNA complexed with single-stranded DNA binding protein (SSB). The annealing of complementary strands bound by SSB requires a ResT-SSB interaction that is mediated by the conserved amphipathic C-terminal tail of SSB. These properties of ResT are similar to those demonstrated for the recombination mediator protein, RecO, of the RecF pathway. Borrelia burgdorferi is unusual in lacking identifiable homologs of the RecFOR proteins. We propose that ResT may provide missing RecFOR functions. PMID:27131360

  2. 姬松茸多糖对树突状细胞Dectin-1、Syk及CARD9基因表达和T淋巴细胞增殖的影响%Effect of Polysaccharide Isolated from Agaricus Blazei Murill on mRNA Expression Levels of Dectin-1, Syk and CARD9 from Dendritic Cells

    Institute of Scientific and Technical Information of China (English)

    董海影; 高志影; 荣华; 金铭; 王俊平; 牛英才; 刘吉成

    2014-01-01

    目的:探讨低相对分子质量姬松茸多糖(low molecular weight polysaccharide isolated from Agaricus blazei Murill,LMPAB)对树突状细胞C型植物血凝素1(Dectin-1),脾酪氨酸激酶(Sky)和胱天蛋白募集域蛋白9(CARD9)基因表达和T淋巴细胞增殖的影响.方法:体外培养ICR小鼠骨髓来源的树突状细胞;通过流式细胞术检测树突状细胞表面CD11c和CD86分子的表达;采用WST方法检测LMPAB增强树突状细胞对T淋巴细胞的增殖作用;应用实时定量PCR方法检测Dectin-1,Sky和CARD9 mRNA的表达水平.结果:树突状细胞表面CD11c表达98.6%,CD86表达96.97%;与正常组(4.84±0.21)比较,LMPAB +OVA组(6.59±0.19)可增强树突状细胞对T淋巴细胞的增殖作用(P<0.05);与正常组Dectin-1 (6.13±0.27),Sky(0.36 ±0.11)和CARD9(1.85±0.20)比较,LMPAB+OVA组Dectin-1(8.25±0.19),Sky(1.63±0.16)和CARD9(4.40±0.19)明显升高,统计学具有显著意义(P<0.05).结论:所培养的髓系细胞为树突状细胞;LMPAB可通过调控树突状细胞Dectin-1,Sky和CARD9 mRNA的表达增强树突状细胞的免疫效能.

  3. Complete genome sequence of Treponema pallidum ssp. pallidum strain SS14 determined with oligonucleotide arrays

    Directory of Open Access Journals (Sweden)

    Sodergren Erica

    2008-05-01

    Full Text Available Abstract Background Syphilis spirochete Treponema pallidum ssp. pallidum remains the enigmatic pathogen, since no virulence factors have been identified and the pathogenesis of the disease is poorly understood. Increasing rates of new syphilis cases per year have been observed recently. Results The genome of the SS14 strain was sequenced to high accuracy by an oligonucleotide array strategy requiring hybridization to only three arrays (Comparative Genome Sequencing, CGS. Gaps in the resulting sequence were filled with targeted dideoxy-terminators (DDT sequencing and the sequence was confirmed by whole genome fingerprinting (WGF. When compared to the Nichols strain, 327 single nucleotide substitutions (224 transitions, 103 transversions, 14 deletions, and 18 insertions were found. On the proteome level, the highest frequency of amino acid-altering substitution polymorphisms was in novel genes, while the lowest was in housekeeping genes, as expected by their evolutionary conservation. Evidence was also found for hypervariable regions and multiple regions showing intrastrain heterogeneity in the T. pallidum chromosome. Conclusion The observed genetic changes do not have influence on the ability of Treponema pallidum to cause syphilitic infection, since both SS14 and Nichols are virulent in rabbit. However, this is the first assessment of the degree of variation between the two syphilis pathogens and paves the way for phylogenetic studies of this fascinating organism.

  4. Symmetry in the changing jets of SS433 and its true distance from us

    CERN Document Server

    Bowler, K B M

    2004-01-01

    We present the deepest yet radio image of the Galactic jet source, SS433, which reveals over two full precession cycles (> 2 x 163 days) of the jet axis. Systematic and identifiable deviations from the traditional kinematical model for the jets are found: variations in jet speed, lasting for as long as tens of days, are necessary to match the detailed structure of each jet. It is remarkable that these variations are equal and opposite, matching the two jets simultaneously. This explains certain features of the correlated redshift residuals found in fits to the kinematic model of SS433 reported in the literature. Asymmetries in the image caused by light travel time enabled us to measure the jet speeds of particular points to be within a range from 0.24c to 0.28c, consistent with, yet determined independently from, the speeds derived from the famous moving optical emission lines. Taken together with the angular periodicity of the zigzag/corkscrew structure projected on the plane of the sky (produced by the prec...

  5. Evolution in Binary and Triple Stars, with an application to SS Lac

    CERN Document Server

    Eggleton, P P; Eggleton, Peter P.; Kiseleva-Eggleton, Ludmila

    2001-01-01

    We present equations governing the way in which both the orbit and the intrinsic spins of stars in a close binary should evolve subject to a number of perturbing forces, including the effect of a third body in a possibly inclined wider orbit. We illustrate the solutions in some binary-star and triple-star situations: tidal friction in a wide but eccentric orbit of a radio pulsar about a B star, the Darwin and eccentricity instabilities in a more massive but shorter-period massive X-ray binary, and the interaction of tidal friction with Kozai cycles in a triple such as Algol (beta-Per), at an early stage in that star's life when all 3 components were ZAMS stars. We also attempt to model in some detail the interesting triple system SS Lac, which stopped eclipsing in about 1950. We find that our model of SS Lac is quite constrained by the relatively good observational data of this system, and leads to a specific inclination (29 deg) of the outer orbit relative to the inner orbit at epoch zero (1912). Although th...

  6. ssDNA Pairing Accuracy Increases When Abasic Sites Divide Nucleotides into Small Groups.

    Directory of Open Access Journals (Sweden)

    Alexandra Peacock-Villada

    Full Text Available Accurate sequence dependent pairing of single-stranded DNA (ssDNA molecules plays an important role in gene chips, DNA origami, and polymerase chain reactions. In many assays accurate pairing depends on mismatched sequences melting at lower temperatures than matched sequences; however, for sequences longer than ~10 nucleotides, single mismatches and correct matches have melting temperature differences of less than 3°C. We demonstrate that appropriately grouping of 35 bases in ssDNA using abasic sites increases the difference between the melting temperature of correct bases and the melting temperature of mismatched base pairings. Importantly, in the presence of appropriately spaced abasic sites mismatches near one end of a long dsDNA destabilize the annealing at the other end much more effectively than in systems without the abasic sites, suggesting that the dsDNA melts more uniformly in the presence of appropriately spaced abasic sites. In sum, the presence of appropriately spaced abasic sites allows temperature to more accurately discriminate correct base pairings from incorrect ones.

  7. X-ray Variability of SS 433: Evidence for Supercritical Accretion

    CERN Document Server

    Atapin, Kirill

    2016-01-01

    We study the X-ray variability of SS433 based on data from the ASCA observatory and the MAXI and RXTE/ASM monitoring missions. Based on the ASCA data, we have constructed the power spectrum of SS433 in the frequency range from $10^{-6}$ to 0.1 Hz, which confirms the presence of a flat portion (flat-topped noise) in the spectrum at frequencies $3\\times 10^{-5}$ - $10^{-3}$ Hz. The periodic variability (precession, nutation, eclipses) begins to dominate significantly over the stochastic variability at lower frequencies, which does not allow the stochastic variability to be studied reliably. The best agreement with the observations is reached by the model with the flat portion extending to $9.5\\times10^{-6}$ Hz and a power-law spectrum with index of 2.6 below that frequency. The jet nutation with a period of about three days suggests that the time for the passage of material through the disk is less than this value. Therefore, at frequencies below $4\\times10^{-6}$ Hz, the power spectrum probably does not reflect...

  8. Complete genome sequence of Treponema pallidum ssp. pallidum strain SS14 determined with oligonucleotide arrays

    Science.gov (United States)

    Matějková, Petra; Strouhal, Michal; Šmajs, David; Norris, Steven J; Palzkill, Timothy; Petrosino, Joseph F; Sodergren, Erica; Norton, Jason E; Singh, Jaz; Richmond, Todd A; Molla, Michael N; Albert, Thomas J; Weinstock, George M

    2008-01-01

    Background Syphilis spirochete Treponema pallidum ssp. pallidum remains the enigmatic pathogen, since no virulence factors have been identified and the pathogenesis of the disease is poorly understood. Increasing rates of new syphilis cases per year have been observed recently. Results The genome of the SS14 strain was sequenced to high accuracy by an oligonucleotide array strategy requiring hybridization to only three arrays (Comparative Genome Sequencing, CGS). Gaps in the resulting sequence were filled with targeted dideoxy-terminators (DDT) sequencing and the sequence was confirmed by whole genome fingerprinting (WGF). When compared to the Nichols strain, 327 single nucleotide substitutions (224 transitions, 103 transversions), 14 deletions, and 18 insertions were found. On the proteome level, the highest frequency of amino acid-altering substitution polymorphisms was in novel genes, while the lowest was in housekeeping genes, as expected by their evolutionary conservation. Evidence was also found for hypervariable regions and multiple regions showing intrastrain heterogeneity in the T. pallidum chromosome. Conclusion The observed genetic changes do not have influence on the ability of Treponema pallidum to cause syphilitic infection, since both SS14 and Nichols are virulent in rabbit. However, this is the first assessment of the degree of variation between the two syphilis pathogens and paves the way for phylogenetic studies of this fascinating organism. PMID:18482458

  9. Interacting Large-Scale Magnetic Fields and Ionised Gas in the W50/SS433 System

    CERN Document Server

    Farnes, J S; Purcell, C; Sun, X H; Haverkorn, M; Lenc, E; O'Sullivan, S P; Akahori, T

    2016-01-01

    The W50/SS433 system is an unusual Galactic outflow-driven object of debatable origin. We have used the Australia Telescope Compact Array (ATCA) to observe a new 198 pointing mosaic, covering $3^\\circ \\times 2^\\circ$, and present the highest-sensitivity full-Stokes data of W50 to date using wide-field, wide-band imaging over a 2 GHz bandwidth centred at 2.1 GHz. We also present a complementary H$\\alpha$ mosaic created using the Isaac Newton Telescope Photometric H$\\alpha$ Survey of the Northern Galactic Plane (IPHAS). The magnetic structure of W50 is found to be consistent with the prevailing hypothesis that the nebula is a reanimated shell-like supernova remnant (SNR), that has been re-energised by the jets from SS433. We observe strong depolarization effects that correlate with diffuse H$\\alpha$ emission, likely due to spatially-varying Faraday rotation measure (RM) fluctuations of $\\ge48$ to 61 rad m$^{-2}$ on scales $\\le4.5$ to 6 pc. We also report the discovery of numerous, faint, H$\\alpha$ filaments tha...

  10. HIV-derived ssRNA binds to TLR8 to induce inflammation-driven macrophage foam cell formation.

    Directory of Open Access Journals (Sweden)

    Mark A Bernard

    Full Text Available Even though combined anti-retroviral therapy (cART dramatically improves patient survival, they remain at a higher risk of being afflicted with non-infectious complications such as cardiovascular disease (CVD. This increased risk is linked to persistent inflammation and chronic immune activation. In this study, we assessed whether this complication is related to HIV-derived ssRNAs inducing in macrophages increases in TNFα release through TLR8 activation leading to foam cell formation. HIV ssRNAs induced foam cell formation in monocyte-derived macrophages (MDMs in a dose-dependent manner. This response was reduced when either endocytosis or endosomal acidification was inhibited by dynasore or chloroquine, respectively. Using a flow cytometry FRET assay, we demonstrated that ssRNAs bind to TLR8 in HEK cells. In MDMs, ssRNAs triggered a TLR8-mediated inflammatory response that ultimately lead to foam cell formation. Targeted silencing of the TLR8 and MYD88 genes reduced foam cell formation. Furthermore, foam cell formation induced by these ssRNAs was blocked by an anti-TNFα neutralizing antibody. Taken together in MDMs, HIV ssRNAs are internalized; bind TLR8 in the endosome followed by endosomal acidification. TLR8 signaling then triggers TNFα release and ultimately leads to foam cell formation. As this response was inhibited by a blocking anti-TNFα antibody, drug targeting HIV ssRNA-driven TLR8 activation may serve as a potential therapeutic target to reduce chronic immune activation and inflammation leading to CVD in HIV+ patients.

  11. SS18 together with animal-specific factors defines human BAF-type SWI/SNF complexes.

    Directory of Open Access Journals (Sweden)

    Evelien Middeljans

    Full Text Available BACKGROUND: Nucleosome translocation along DNA is catalyzed by eukaryotic SNF2-type ATPases. One class of SNF2-ATPases is distinguished by the presence of a C-terminal bromodomain and is conserved from yeast to man and plants. This class of SNF2 enzymes forms rather large protein complexes that are collectively called SWI/SNF complexes. They are involved in transcription and DNA repair. Two broad types of SWI/SNF complexes have been reported in the literature; PBAF and BAF. These are distinguished by the inclusion or not of polybromo and several ARID subunits. Here we investigated human SS18, a protein that is conserved in plants and animals. SS18 is a putative SWI/SNF subunit which has been implicated in the etiology of synovial sarcomas by virtue of being a target for oncogenic chromosomal translocations that underlie synovial sarcomas. METHODOLOGY/PRINCIPAL FINDINGS: We pursued a proteomic approach whereby the SS18 open reading frame was fused to a tandem affinity purification tag and expressed in amenable human cells. The fusion permitted efficient and exclusive purification of so-called BAF-type SWI/SNF complexes which bear ARID1A/BAF250a or ARID1B/BAF250b subunits. This demonstrates that SS18 is a BAF subtype-specific SWI/SNF complex subunit. The same result was obtained when using the SS18-SSX1 oncogenic translocation product. Furthermore, SS18L1, DPF1, DPF2, DPF3, BRD9, BCL7A, BCL7B and BCL7C were identified. 'Complex walking' showed that they all co-purify with each other, defining human BAF-type complexes. By contrast,we demonstrate that human PHF10 is part of the PBAF complex, which harbors both ARID2/BAF200 and polybromo/BAF180 subunits, but not SS18 and nor the above BAF-specific subunits. CONCLUSIONS/SIGNIFICANCE: SWI/SNF complexes are found in most eukaryotes and in the course of evolution new SWI/SNF subunits appeared. SS18 is found in plants as well as animals. Our results suggest that in both protostome and deuterostome animals

  12. Corrosion-erosion test of SS316L grain boundary engineering material (GBEM) in lead bismuth flowing loop

    Science.gov (United States)

    Saito, Shigeru; Kikuchi, Kenji; Hamaguchi, Dai; Tezuka, Masao; Miyagi, Masanori; Kokawa, Hiroyuki; Watanabe, Seiichi

    2012-12-01

    To evaluate the lifetime of structural materials utilized in a spallation neutron source, corrosion tests in lead-bismuth eutectic (LBE) have been done at JAEA. Austenitic steels are preferable as the structural material for ADS. However, previous studies have revealed that austenitic steel SS316 shows severe corrosion-erosion in LBE because of LBE penetration through grain boundaries and separation of grains. So it was considered that GBE (grain-boundary engineered) materials may be effective to improve the corrosion resistance of austenitic steels in LBE. In this study, the results of corrosion tests on austenitic steel SS316L-BM (base metal) and SS316L-GBEM (grain-boundary-engineered material) under flowing LBE conditions will be reported. The corrosion test was performed using the JAEA lead-bismuth material corrosion loop (JLBL-1). The experimental conditions were as follows: The high and low temperature parts of the loop were 450 °C and 350 °C, respectively. The flow velocity at the test specimens was about 0.7 m/s. The oxygen concentration in LBE was not controlled and was estimated to have been very low. After the 3600 h of operation, macroscopic, SEM, and SIM observations and EDX analysis were carried out. The results showed that the corrosion depth and LBE penetration through the grain boundaries of the 316SS-GBEM were smaller than those of the 316SS-BM.

  13. Corrosion-erosion test of SS316L grain boundary engineering material (GBEM) in lead bismuth flowing loop

    Energy Technology Data Exchange (ETDEWEB)

    Saito, Shigeru, E-mail: saito.shigeru@jaea.go.jp [JAEA, Tokai-mura, Ibaraki-ken 319-1195 (Japan); Kikuchi, Kenji [Ibaraki Univ., Frontier Research Center, Tokai-mura, Ibaraki-ken 319-1106 (Japan); Hamaguchi, Dai [JAEA, Tokai-mura, Ibaraki-ken 319-1195 (Japan); Tezuka, Masao [Sukegawa Electic Co., Takahagi-shi, Ibaraki-ken 318-0004 (Japan); Miyagi, Masanori; Kokawa, Hiroyuki [Tohoku Univ., Sendai-shi, Miyagi-ken 980-8579 (Japan); Watanabe, Seiichi [Hokkaido Univ., Sapporo-shi, Hokkai-do 060-8628 (Japan)

    2012-12-15

    To evaluate the lifetime of structural materials utilized in a spallation neutron source, corrosion tests in lead-bismuth eutectic (LBE) have been done at JAEA. Austenitic steels are preferable as the structural material for ADS. However, previous studies have revealed that austenitic steel SS316 shows severe corrosion-erosion in LBE because of LBE penetration through grain boundaries and separation of grains. So it was considered that GBE (grain-boundary engineered) materials may be effective to improve the corrosion resistance of austenitic steels in LBE. In this study, the results of corrosion tests on austenitic steel SS316L-BM (base metal) and SS316L-GBEM (grain-boundary-engineered material) under flowing LBE conditions will be reported. The corrosion test was performed using the JAEA lead-bismuth material corrosion loop (JLBL-1). The experimental conditions were as follows: The high and low temperature parts of the loop were 450 Degree-Sign C and 350 Degree-Sign C, respectively. The flow velocity at the test specimens was about 0.7 m/s. The oxygen concentration in LBE was not controlled and was estimated to have been very low. After the 3600 h of operation, macroscopic, SEM, and SIM observations and EDX analysis were carried out. The results showed that the corrosion depth and LBE penetration through the grain boundaries of the 316SS-GBEM were smaller than those of the 316SS-BM.

  14. The eSS rat, a nonobese model of disordered glucose and lipid metabolism and fatty liver

    Directory of Open Access Journals (Sweden)

    Daniele Stella M

    2010-03-01

    Full Text Available Abstract Background eSS is a rat model of type 2 diabetes characterized by fasting hyperglycemia, glucose intolerance, hyperinsulinemia and early hypertriglyceridemia. Diabetic symptoms worsen during the second year of life as insulin release decreases. In 12-month-old males a diffuse hepatic steatosis was detected. We report the disturbances of lipid metabolism of the model with regard to the diabetic syndrome. Methods The study was conducted in eight 12-month-old eSS male rats and seven age/weight matched eumetabolic Wistar rats fed with a complete commercial diet al libitum. Fasting plasmatic glucose, insulin, triglycerides, total cholesterol, low-density and high-density lipoprotein, and nonesterified fatty acids levels were measured. Very low density and intermediate-density lipoproteins were analyzed and hepatic lipase activity was determined. Results eSS rats developed hyperglycemia and hyperinsulinemia, indicating insulin resistance. Compared with controls, diabetic rats exhibited high plasmatic levels of NEFA, triglycerides (TG, total cholesterol (Chol and LDL-Chol while high-density lipoprotein (HDL cholesterol values were reduced. eSS rats also displayed TG-rich VLDL and IDL particles without changes in hepatic lipase activity. Conclusion The nonobese eSS rats develop a syndrome characterized by glucose and lipid disorders and hepatic steatosis that may provide new opportunities for studying the pathogenesis of human type 2 diabetes.

  15. "Toivomushan ois, et tää ois niinku jokaisen perheen olohuone" : Suviniityn avoin päiväkoti ennaltaehkäisevässä perhetyössä

    OpenAIRE

    Latvala, Marika

    2010-01-01

    Marika Latvala ”Toivomushan ois, et tää ois niinku jokaisen perheen olohuone” Suviniityn avoin päiväkoti ennaltaehkäisevässä perhetyössä Tämän kvalitatiivisesti toteutetun opinnäytetyön tarkoituksena on arvioida Espoossa sijaitsevan Suviniityn avoimen päiväkodin merkitystä asiakasperheiden elämässä ja työntekijöiden näkemyksiä avoimessa päiväkodissa tehtävästä perhetyöstä. Tavoitteina on ollut selvittää myös palvelun kehittämistarpeet sekä sen toteuttamisen esteet ja mahdollisuude...

  16. Comparison of different joint configurations between two parallel SS-laminated BSCCO/Ag tapes

    International Nuclear Information System (INIS)

    Stainless steel laminated BSCCO/Ag tapes are commercially available for HTS pancake magnet development. The joint between pancakes could be achieved in several configurations like straight lap joint, cross-bridge joint and combination of both. Each type of joint shows different characteristic. We have experimentally studied the characteristic of three types of joint configuration between two pancakes made of SS-laminated BSCCO/Ag tape at 77 K. We have developed two types of cross-bridge joint and one with combination of both straight lap joint and cross-bridge joint. The joint resistance for all three configurations has been compared. The rise in temperature at each joint has been estimated. The joint configuration with the combination of both straight lap joint and cross-bridge joint has given lowest joint resistance which is 0.25 μΩ at 77 K but this configuration has more locally heated zones.

  17. Blood groups polymorphism (ABO, Ss, Rhesus and Duffy) in the Arabic population of Beni Mellal

    International Nuclear Information System (INIS)

    The present study deals with anthropogenetic profile of the Arab speaking population of the Beni Mellal region which separates areas inhabited by Mid-Atlas Berbers from those inhabited by Soth-Morroccan Arabs. The study of blood groups ABO, Rhesus, Ss,and Duffy was conducted on 131 individuals. The result shows that this population has the highest frequencies of the FyO allele (0.860) and s allele (0.524) in comparison to all Arab and Berber populations of North Africa and the Middle East. However genetic distances estimated on the basis of these four markers reveal that the population of Beni Mellal and another in the Beni Hlal region are in the same sub-cluster with populations from the Middle East. This may be attributed to the Oriental Arab (Machrek) origin of these two Moroccan Arab populations. (author)

  18. 5S:n käyttöönotto Nomet Oy:ssä

    OpenAIRE

    Nummi, Janne

    2012-01-01

    Tämän opinnäytetyön aiheena on 5S-toimintamallin käyttöönotto Nomet Oy:n tuotannossa, Tampereen Vehmaisissa. Nomet on alihankintakonepaja, joka on erikoistunut lastuavaan työstöön. Yrityksessä on päätetty ottaa käyttöön Lean- valmistusfilosofiaan pohjautuvaa tuotannon uudistamista. 5S on yksi Leanin monista työkaluista, josta on hyvä lähteä alkuun. 5S:n päätavoitteena on parantaa tuotantoprosesseja siisteyden, järjestyksen ja standardisoinnin avulla. Työssä on esitelty kahdesta tuotannon ...

  19. Purification and preliminary characterization of a xylanase from Thermomyces lanuginosus strain SS-8.

    Science.gov (United States)

    Shrivastava, Smriti; Shukla, Pratyoosh; Mukhopadhyay, Kunal

    2011-12-01

    Thermomyces lanuginosus SS-8 was isolated from soil samples that had been collected from near self-heating plant material and its extracellular cellulase-free xylanase purified approximately 160-fold using ion exchange chromatography and continuous elution electrophoresis. This xylanase was thermoactive (optimum temperature 60 °C) at pH 6.0 and had a molecular weight of 23.79 kDa as indicated by SDS-PAGE electrophoresis. The xylanase rapidly hydrolyzed xylan directly to xylose without the production of intermediary xylo-oligosaccharides within 15 min of incubation under optimum conditions. This trait of rapidly degrading xylan to xylose as a sole end-product could have biotechnological potential in degradation of agro-wastes for bioethanol manufacturing industry. PMID:22558544

  20. DotA传奇人物回顾:ToSsGirL

    Institute of Scientific and Technical Information of China (English)

    2010-01-01

    首先声明,此ToSsGirL不是彼TossGirl,他没有前凸后翘的身材也没有令韩国众宅男YY不已的脸蛋,他只是一个深爱DotA最后却不得不放弃DotA的,为梦想不断前行的DotA玩家。或许现在的DotA爱好者们并不熟悉这个人,甚至都从来没听说过这个ID,但这并不妨碍他在中国DotA的历史中写下浓墨重彩的一笔,也并不妨碍他开启中国电子竞技的新篇章。

  1. Isännöitsijänä asunto-osakeyhtiössä

    OpenAIRE

    Alajärvi, Annika

    2015-01-01

    Tämän opinnäytetyön aiheena oli isännöitsijänä asunto-osakeyhtiössä. Tutkimustehtävänä oli tarkastella isännöitsijän ominaisuuksia, ammattitaidon ylläpitämistä, tehtäväkenttää sekä hyvän isännöitsijän piirteitä. Tarkastelun kohteena olivat asunto-osakeyhtiöiden isännöitsijät ja tutkimus perustui isännöitsijöiden näkemyksiin tutkimusaiheesta. Tutkimuskysymykset olivat: minkälaisia henkilöitä toimii isännöitsijöinä, miten isännöitsijä ylläpitää ammattitaitoaan, millainen on isännöitsijän tehtäv...

  2. Luomu- ja lähiruoka-aamiaisten suunnittelu, toteutus ja seuranta yritys X:ssä

    OpenAIRE

    Kasurinen, Antero

    2010-01-01

    Opinnäytetyö toteutettiin toimeksiantona yritys X:ään. Yritys X halusi vastata asiakkaiden tarpeisiin ‎kehittämällä jo olemassa olevien aamiaisten rinnalle sekä luomu- että lähiruokaaamiaisen.lisäksi ‎opinnäytetyön tavoitteena oli tarkastella luomu- ja lähiruoka-aamiaisten myyntiä touko-joulukuun 2009 ‎ajalta. Opinnäytetyön pääongelmaksi muodostui, miten suunnitellaan, toteutetaan ja seurataan luomu- ja ‎lähiruoka-aamiaisia yritys X:ssä. Opinnäytetyön teoriaosassa käsitellään puolestaan luomu...

  3. Työkierto Meilahden sairaalan fysiatrian yksikössä

    OpenAIRE

    Mökkönen, Tiina; Ojala, Eeva

    2008-01-01

    Kehittämistyö tukee Meilahden sairaalan fysiatrian yksikön työhyvinvointia edistävää toimintaa. Eri työntekijöillä on syvällistä ammatillista osaamista omalta erikoisalaltaan ja sitä pitäisi pystyä jakamaan laajemmin koko työyhteisössä. Kehittämistyön aihe syntyi työyhteisön tarpeiden pohjalta ja sen tarkoituksena oli parantaa Meilahden sairaalan fysioterapeuttien laaja-alaista ammatillista osaamista sekä työyhteisön toimivuutta ja työhyvinvointia työkierron avulla. Tavoitteena oli arvio...

  4. Myyntihenkilöstön rekrytoinnin kehittäminen Yritys Oy:ssä

    OpenAIRE

    Vatka-Horttanainen, Satu

    2011-01-01

    Tämän opinnäytetyön tarkoituksena oli kehittää Yritys Oy:n myyntihenkilöstön rekrytointia. Opinnäytetyössä luotiin kehitysehdotuksia myös myyntihenkilöstön henkilöstövaihtuvuuden pienentämiseksi. Tavoitteena oli löytää hyviä käytäntöjä ja menetelmiä myyntihenkilöstön hankintaan ja yrityksen houkuttelevuuteen. Tutkimuksen teoreettinen tietoperusta ja viitekehys rakennettiin henkilöstövoimavarojen strategista johtamista sekä rekrytointia koskevasta liiketalouden kirjallisuudesta. Se rakentu...

  5. Unusual stabilization behavior in Type 347H SS tubulars for a refinery heater

    Energy Technology Data Exchange (ETDEWEB)

    Boliner, S.R. [Shell Norco Refining Co., Norco, LA (United States); Kolp, L.E.; Fort, W.C. III [Shell Oil Products Co., Dear Park, TX (United States)]|[Shell Oil Products Co., Houston, TX (United States)

    1998-12-31

    Type 347H SS was specified for the tubulars in a Refinery Lube Oil Vacuum Flasher Heater. The heater was designed for tube metal temperatures of 565 C (1,050 F), and the tubes were specified to be stabilized annealed in order to resist polythionic acid stress corrosion cracking (PTA-SCC). Testing was performed to determine if resistance to PTA-SCC had been compromised during fabrication of the heater. The test protocol was based on ASTM A-262 Practice C, the 65% nitric acid (Huey) test. All return bends failed the tests, while most of the straight tubes sections passed the tests. The columbium carbide phase was present in very large particles instead of being evenly dispersed as fine particles throughout the matrix. This compromised the effectiveness of the stabilizing anneal and reduced the resistance to PTA-SCC.

  6. PREFACE: The 4th Symposium on the Mechanics of Slender Structures (MoSS2013)

    Science.gov (United States)

    Cao, Dengqing; Kaczmarczyk, Stefan

    2013-07-01

    This volume of Journal of Physics: Conference Series contains papers presented at the 4th Symposium on the Mechanics of Slender Structures (MoSS2013) run under the auspices of the Institute of Physics Applied Mechanics Group and hosted by Harbin Institute of Technology (China) from 7-9 January 2013. The conference has been organized in collaboration with the Technical Committee on Vibration and Sound of the American Society of Mechanical Engineers and follows a one day seminar on Ropes, Cables, Belts and Chains: Theory and Applications and the MoSS2006 symposium held at the University of Northampton (UK) in 2004 and 2006, respectively, the MoSS2008 symposium held at the University of Maryland Baltimore County (USA) in 2008 and the MoSS2010 symposium hosted by Mondragon University and held in San Sebastian (Spain) in 2010. The remit of the Symposium on the Mechanics of Slender Structures series involves a broad range of scientific areas. Applications of slender structures include terrestrial, marine and space systems. Moving elastic elements such as ropes, cables, belts and tethers are pivotal components of many engineering systems. Their lengths often vary when the system is in operation. The applications include vertical transportation installations and, more recently, space tether propulsion systems. Traction drive elevator installations employ ropes and belts of variable length as a means of suspension, and also for the compensation of tensile forces over the traction sheave. In cranes and mine hoists, cables and ropes are subject to length variation in order to carry payloads. Tethers experiencing extension and retraction are important components of offshore and marine installations, as well as being proposed for a variety of different space vehicle propulsion systems based on different applications of momentum exchange and electrodynamic interactions with planetary magnetic fields. Furthermore, cables and slender rods are used extensively in civil engineering

  7. Sosiaalisen median hyödyntäminen pelastustoimen ulkoisessa viestinnässä

    OpenAIRE

    Sipola, Marko

    2015-01-01

    Suomessa sosiaalisen median käyttö on kasvanut. Sitä ei enää käytä pelkästään nuoriso, vaan siitä on kasvanut koko väestön vuorovaikutuskanava. Nykyisin erilaiset yhteisöpalvelut kasvavat nopeammin kuin muut median palvelut Suomessa. Tämän opinnäytetyön aiheena oli tutkia pelastuslaitosten sosiaalisen median hyödyntämistä ulkoisessa viestinnässä. Tutkimuksen aiheena oli tarkastella, miten pelastuslaitokset hyödyntävät sosiaalista mediaa nykyisin, ja tutkia, miten sitä voisi hyödyntää tule...

  8. Prediction of IGSCC in Type 304 SS using an artificial neural network

    International Nuclear Information System (INIS)

    Intergranular stress corrosion cracking (IGSCC) of recirculating piping in boiling water reactors (BWRs) has been a major operating problem world-wide. An artificial neural network (ANN) has been developed to describe intergranular stress corrosion cracking in sensitized Type 304SS in high temperature aqueous solutions. The ANN predictions of crack growth rate (CGR) versus oxygen concentration, flow velocity, stress intensity, hydrogen concentration, and ECP are compared with the predictions of the deterministic Coupled Environment Fracture Model (CEFM). The predictions of these two approaches, which represent the extremes in the spectrum of predictive technologies, are generally in good accord, except that the CGRs obtained from the ANN are up to a factor of three higher than those predicted by the CEFM under some conditions. However, this difference is within the uncertainty in the experiment data used to train the ANN. 39 refs

  9. Tiimityön kehittäminen ministeriössä

    OpenAIRE

    Kuosmanen, Jaana

    2012-01-01

    Tämän opinnäytetyön toimeksiantajana on Valtiovarainministeriön Hallinnon kehittä-misosasto (nykyisin uudelleenorganisoitunut), joka on kehittänyt tiimityöskentelyään. Opinnäytetyön tarkoituksena oli selvittää, minkälaisia tiimityön teorioita ja oppeja Hallinnon kehittämisosasto on ottanut esille kehittämistyössään, ja tutkia niiden soveltuvuutta käytännön hanketyöhön, jota on tarkoitus toteuttaa tiimimäisesti. Tämän työn teoreettisessa viitekehyksessä käsitellään sellaisia tiimeihin ja t...

  10. Mycobacterium avium ss paratuberculosis-associated diseases: piecing the Crohn's puzzle together.

    Science.gov (United States)

    Gitlin, Laura; Borody, Thomas Julius; Chamberlin, William; Campbell, Jordana

    2012-09-01

    The relation of Mycobacterium avium ss paratuberculosis (MAP) to Crohn's Disease (CD) and other MAP-associated conditions remains controversial. New data, coupled with the analogous Helicobacter pylori (H. pylori) story, has permitted us to piece together the MAP puzzle and move forward with a more scientific way of treating inflammatory bowel disease, particularly CD. As infection moves centre stage in inflammatory bowel disease, the dated "aberrant reaction" etiology has lost scientific credibility. Now, our growing understanding of MAP-associated diseases demands review and articulation. We focus here on (1) the concept of MAP-associated diseases; (2) causality, Johne Disease, the "aberrant reaction" hypothesis; and (3) responses to published misconceptions questioning MAP as a pathogen in CD. PMID:22858515

  11. Sovellettu väriteoria 3D-ympäristössä

    OpenAIRE

    Enges, Iiro

    2012-01-01

    Tämä opinnäyte on kirjoittajan henkilökohtainen kehittämisprojekti, jossa pyritään tutkimaan käytännön väriteoriaa kuvakerronnassa ja soveltamaan sitä digitaalisen 3D-ympäristön työskentelyyn. Työn tavoitteena on tutkia käytännönläheisesti, millaisia värejä tehokkaan kuvan tekoon käytetään ja miten ne voidaan tietoisten valintojen kautta saavuttaa 3D-ympäristössä. Tekstiosuudessa keskitytään värin ja siihen liittyvien teorioiden selittämiseen, käsitellen värin fysikaalisia, visuaalisia ja...

  12. Reweldability test of irradiated SS316 by the TIG welding method

    Science.gov (United States)

    Tsuchiya, Kunihiko; Kawamura, Hiroshi; Oyamada, Rokuro

    1996-10-01

    Stainless steel is a candidate material for the structural material in fusion reactors. Rewelding of irradiated materials will have a large impact on the design and the maintenance of in-vessel components. In the present work, the welding specimens made of type 316 stainless steel were irradiated in JMTR (Japan materials testing reactor) to a fast neutron fluence of ˜2.0 × 10 20 n/cm 2 ( E > 1 MeV) at a temperature of ˜200°C. The rewelding of unirradiated and/or irradiated stainless steel was performed by the tungsten inert gas (TIG) welding method and the weldments of unirradiated and/or irradiated SS316 were characterized by tensile testing (test temp.: 20°C and 200°C), hardness, metallographical observation and SEM/XMA analyses.

  13. Migration and release behavior of tritium in SS316 at ambient temperature

    Science.gov (United States)

    Torikai, Y.; Murata, D.; Penzhorn, R.-D.; Akaishi, K.; Watanabe, K.; Matsuyama, M.

    2007-06-01

    BIXS measurements indicate that immersion into water or chemical etching of SS316 contaminated with tritium at moderate temperatures causes an immediate reduction of the outermost surface concentration of tritium. The fraction of surface tritium removed by water, i.e. 30-50%, is small in comparison to the total tritium present in the specimen. Allowing a specimen to age whose surface and subsurface had been removed by etching up to a depth where the concentration of tritium is mostly constant revealed that within a few months a re-growth of tritium up to a saturation value higher than half of that originally present on the specimen takes place. Concurrently, a small but steady liberation of tritium at rates increasing from 0.1 to 0.3 kBq/h was noticed.

  14. Effect of microstructure on radiation induced segregation and depletion in ion irradiated SS316 steel

    Energy Technology Data Exchange (ETDEWEB)

    Jin, Hyung Ha; Kwon, Sang Chul; Kwon, Jun Hyun [Korea Atomic Energy Research Institute, Daejeon (Korea, Republic of)

    2011-10-15

    Irradiation assisted stress corrosion cracking (IASCC), void swelling and irradiation induced hardening are caused by change of characteristics of material by neutron irradiation, stress state of material and environmental situation. It has been known that chemical compositions varies at grain boundary (GB) significantly with fluence level and the depletion of Cr element at GB has been considered as one of important factors causing material degradation, especially, IASCC in austenitic stainless steel. However, experimental results of IASCC under PWR condition were directly not connected with Cr depletion phenomenon by neutron irradiation. Because the mechanism of IASCC under PWR has not yet been clearly understood in spite of many energetic researches, fundamental researches about radiation induced segregation and depletion in irradiated austenitic stainless steels have been attracted again. In this work, an effect of residual microstructure on radiation induced segregation and depletion of alloy elements at GB was investigated in ion irradiated SS316 steel using transmission electron microscope (TEM) with energy dispersive spectrometer (EDS)

  15. On-shell Diagrams, Gra{\\ss}mannians and Integrability for Form Factors

    CERN Document Server

    Frassek, Rouven; Nandan, Dhritiman; Wilhelm, Matthias

    2015-01-01

    We apply on-shell and integrability methods that have been developed in the context of scattering amplitudes in N=4 SYM theory to tree-level form factors of this theory. Focussing on the colour-ordered super form factors of the chiral part of the stress-energy multiplet as an example, we show how to systematically construct on-shell diagrams for these form factors with the minimal form factor as an additional building block. Moreover, we obtain analytic representations in terms of Gra{\\ss}mannian integrals in spinor helicity, twistor and momentum twistor variables. While Yangian invariance is broken by the operator insertion, we find that form factors are eigenstates of the integrable transfer matrix. As a consequence, we can construct them via the method of R operators, which also allows to introduce deformations that preserve the integrable structure.

  16. Uudistuva johtajuus ja Z-sukupolvi työelämässä

    OpenAIRE

    Aakkula, Eveliina

    2012-01-01

    Opinnäytetyössä käsitellään epäjohtamista, joka on uusi johtamisen käsite, jonka ovat luoneet Janne Tienari ja Rebekka Piekkari kirjassaan Z ja epäjohtaminen. Terminä epäjohtaminen ei ole virallinen, eikä tule välttämättä vakiintumaan johtajuuden käsitteiden joukkoon. Tämä uusi johtamisen malli liitetään vahvasti Z-sukupolven tulemiseen työmarkkinoille. Z-sukupolvea ovat 1990-luvulla syntyneet. Johtajuus on tärkeä aihe, jonka käsittelemiseen löytyy aina uusia näkökulmia, joiden toimivuutta vo...

  17. The phase change in 316L S.S. irradiated at 500 degree C

    International Nuclear Information System (INIS)

    The home made 316L S.S. was irradiated by 1.5 MeV proton and 1.8 MeV alpha particle respectively. The phase stability in irradiated samples was investigated by X-ray diffraction. No new phase was found in the specimen which was irradiated by 1.8 MeV alpha particles. But some new phases such as Cr2O3, (CrM)N were found in the specimens irradiated by 1.5 MeV proton. The content of new phases is increased with the doses. The new phase are all the compound of element O, N, S, C and base element Fe, Cr, Ni, Mo

  18. Yksityisyyden suoja työelämässä

    OpenAIRE

    Korttinen, Kaisa

    2011-01-01

    Tämän opinnäytetyön aiheena on yksityisyyden suoja työelämässä. Opinnäyte-työ sisältää periaatteet henkilötietojen käsittelystä työelämän eri vaiheissa, ku-ten työnhaun ja työsuhteen aikana. Opinnäytetyön tarkoituksena on kerätä hyvä tietopaketti työelämän henkilötietojen käsittelyyn liittyvistä laeista ja niihin liitty-vistä perusperiaatteista. Opinnäytetyön teoriaosuus perustuu lainsäädäntöön, joka sisältää työnantajan velvollisuudet rekisterinpitäjänä ja työntekijöiden oikeudet rekiste...

  19. Kansanedustajien avustajien työmotivaatio eduskuntaryhmässä

    OpenAIRE

    Kiuttu, Mikko

    2014-01-01

    Tämän opinnäytetyön aiheena on kansanedustajien avustajien työmotivaatio eduskunta-ryhmässä. Tutkimuksen tarkoituksena on selvittää, mitkä tekijät motivoivat erään edus-kuntaryhmän kansanedustajien avustajia. Työn tavoitteena on myös etsiä ratkaisuja, joilla työmotivaatiota voidaan lisätä. Opinnäytetyön tulokset auttavat osaltaan vielä paremman esimiestyön ja työyhteisön toimivuuden luomisessa. Opinnäytetyön teoreettisena viitekehyksenä on työmotivaation eri osa-alueita. Maslow´n tarvehi...

  20. Jälkilaskennan kehittäminen Jussila Group Oy:ssä

    OpenAIRE

    Tirkkonen, Teemu

    2014-01-01

    Opinnäytetyön tavoitteena on kehittää Jussila Group Oy:ssä maanrakennushankkeiden jälkilaskentaa. Jälkilaskenta on jäänyt vähälle huomiolle yrityksessä ja tähän haluttiin saada muutos. Opinäytetyön tarkoituksena on tarkastella maanrakennushankkeen kustannuslaskelman ja jälkilaskennan kustannusten eroja ja syitä. Jälkilaskentatietojen keräämisellä ja siirtämisellä tarjouslaskennan käyttöön tulevissa urakoissa on yritykselle tärkeä osa. Tähän osa alueeseen kehitettiin excell-pohjainen jälk...

  1. Sopimushallinnan kehittäminen Sastamalan koulutuskuntayhtymässä

    OpenAIRE

    Kurkinen, Heli

    2015-01-01

    Tämä opinnäytetyö tehtiin Sastamalan koulutuskuntayhtymän toimeksiantona. Tämän opinnäytetyön tavoitteena oli selvittää, miten kohdeorganisaation sopimushallinta voidaan järjestää tehokkaammaksi. Yritysten ja julkisyhteisöjen liiketoiminta rakentuu pitkälti sopimusten ympärille. Mitä enemmän organisaation toiminta perustuu erilaisten sopimusvelvoitteiden täyttämiseen, sitä enemmän kasvaa myös sopimusten hallinnoinnin merkitys. Työssä käydään läpi sopimusoikeuden perusteita, mitä etuja hy...

  2. Jet propulsion of wind ejecta from a major flare in the black hole microquasar SS433

    CERN Document Server

    Blundell, Katherine

    2011-01-01

    We present direct evidence, from Adaptive-Optics near-infra-red imaging, of the jets in the Galactic microquasar SS433 interacting with enhanced wind-outflow off the accretion disc that surrounds the black hole in this system. Radiant quantities of gas are transported significant distances away from the black hole approximately perpendicular to the accretion disc from which the wind emanates. We suggest that the material that comprised the resulting "bow-tie" structure is associated with a major flare that the system exhibited ten months prior to the observations. During this flare, excess matter was expelled by the accretion disc as an enhanced wind, which in turn is "snow-ploughed", or propelled, out by the much faster jets that move at approximately a quarter of the speed of light. Successive instances of such bow-ties may be responsible for the large-scale X-ray cones observed across the W50 nebula by ROSAT.

  3. FIGHTING! : Yhteisöllisyys Kpop Finland faniryhmässä

    OpenAIRE

    Sala, Iinamaija

    2013-01-01

    Opinnäytetyön tavoitteena oli selvittää, kuinka yhteisöllisyys rakentuu verkossa toimivassa faniyhteisössä, miten fanit itse kokevat oman verkossa toimivan ryhmänsä sekä mitkä tekijät vaikuttavat kyseisen verkkoyhteisön käyttäjien ryhmään kuuluvuuden tunteeseen. Opinnäytetyö oli tapaustutkimus, jossa faniyhteisön jäsenet vastasivat verkkokyselylomakkeeseen. Lomakkeen avulla kerättiin sekä tilastollista että laadullista tietoa käyttäjien faniudesta, ryhmään kuuluvuudesta sekä yhteisöllisyy...

  4. PCS-kuvien käyttöönotto kahdessa päiväkotiryhmässä

    OpenAIRE

    Kallioinen, Annika

    2015-01-01

    Opinnäytetyöni aiheena oli PCS -kuvien käyttöönotto kahdessa Porin päiväkotiryhmässä. PCS- kuvat (Picture Communication Symbols) ovat usein käytössä integroiduissa päiväkotiryhmissä, mutta tavallisissa ryhmissä niiden käyttö on vielä vähäistä. Kuitenkin lähes jokaisessa ryhmässä on nykyään erityistä tukea tarvitsevia lapsia tai maahanmuuttajalapsia, jotka tarvitsevat kuvia tuomaan apua ja tukea kommunikoinnissaan. Opinnäytetyöni tarkoitus oli auttaa kahden eri päiväkodin tavallista ryhmää ott...

  5. 葡萄糖可调控pSS-SPCAD-proinsulin载体的构建及鉴定

    Institute of Scientific and Technical Information of China (English)

    刘艳霞; 胡平; 栗夏连

    2009-01-01

    目的 构建一葡萄糖可调控pSS-SPCAD-proinsulin表达载体,模仿胰岛β细胞生理功能.方法 从PhIns-ABC扩增出SS和proinsulin,与pEGFP-N1,一人工合成多核苷酸(SPCAD),通过酶切、连接、转化,构建出pSS-SPCAD-proinsulin载体.结果 经PCR,测序证实,pSS-SPCAD-proinsulin栽体构建成功.结论 该表达载体的构建为在胰岛素的分泌水平上实现葡萄糖对胰岛素的调控提供了材料.

  6. Nykyaikaisten mobiililaitteiden tietoturva yrityskäytössä: case: Etelä-Savon Tietohallinto Oy

    OpenAIRE

    Kinnaslampi, Emppu

    2012-01-01

    Tämän opinnäytetyön tarkoituksena oli tutkia, minkälaisia tietoturvauhkia nykyaikaisiin mobiililaitteisiin, kuten älypuhelimiin ja tabletteihin kohdistuu erityisesti yrityskäytössä, ja miten näitä uhkia voidaan torjua ja ennaltaehkäistä. Työssä pyrin selvittämään, miten sekä organisaatio että käyttäjä itse voivat mobiililaitteidensa tietoturvaa aktiivisesti omilla toimillaan parantaa sekä minkälaiset valmiudet nykyään merkittävimmissä mobiilikäyttöjärjestelmissä Androidissa, iOS:ssä, Windows ...

  7. Development, Methodology and Potential of the New Universal Visual Scoring System (UniViSS for Caries Detection and Diagnosis

    Directory of Open Access Journals (Sweden)

    Thomas Oehme

    2009-09-01

    Full Text Available Given the limitations of adjunct caries detection and diagnostic tools, e.g., imperfect validity and reproducibility, as well as the difficulties in controlling all possible confounding factors, the need for an objective visual caries detection and diagnosis system has become evident. Our work has therefore aimed at systematizing caries lesions with the Universal Visual Scoring System (UniViSS for occlusal and smooth surface lesions, which can be used for primary and permanent teeth, as well as under clinical, epidemiological, public health and laboratory conditions. Besides the description of the development and methodology of UniViSS, it is shown that UniViSS allows an accurate and reproducible classification of caries lesions on occlusal surfaces.

  8. Effect of renewed SS-cream on spinal somatosensory evoked potential in rabbits

    Institute of Scientific and Technical Information of China (English)

    LongTian; Zhong-ChengXin; HuaXin; JieFu; Yi-MingYuan; Wu-JiangLiu; ChunYang

    2004-01-01

    Aim:The effect of a renewed SS-cream (RSSC) on the treatment of premature ejaculation (PE) was evaluated and compared with the original SS-cream (OSSC).Methods: Sixty male white New Zealand rabbits,weighing 2.5kg-3.0kg,were divided at random into 3 groups:the RSSC,OSSC and placebo groups.The spinal somatosensory evoked potential (SSEP) elicited by electric stimulation of the glans penis with disk electrode was investigated with an electrophysiograph (Poseidomn,Shanghai,China) before and 10,30 and 60 min after drug or placebo application on the glans.The Onset and the N1 latencies and the amplitude of SSEP were recorded and analyzed. Results:There was no significant difference (P>0.05) in the mean Onset and N1 latency of SSEP among the 3 groups before drug application.Compared with the pre-application value,the mean Onset and N 1 latencies in the RSSC and OSSC groups were significantly prolonged at 10,30 and 60 min after treatment (P0.05) in the placebo group.The mean Onset latency of RSSC at 10 and 30 min and that of OSSC at 30 min were significantly delayed (P<0.05) compared with the placebo group.The mean N1 latency of RSSC at 30 and 60 min and that of OSSC group at 30 min were also significantly delayed (P<0.05).Conclusion:RSSC delays the latencies of SSEP,suggesting a local desensitizing effect on the sensory receptor of the glans penis dorsal nerve,which provides the potential for PE treatment.The desensitizing effect of RSSC is higher than that of OSSC.( Asian J Androl 2004 Mar;6:15-18)

  9. The effect of topography of upper-mantle discontinuities on SS precursors

    Science.gov (United States)

    Koroni, Maria; Trampert, Jeannot

    2016-01-01

    Using the spectral-element method, we explored the effect of topography of upper-mantle discontinuities on the traveltimes of SS precursors recorded on transverse component seismograms. The latter are routinely used to infer the topography of mantle transition zone discontinuities. The step from precursory traveltimes to topographic changes is mainly done using linearised ray theory, or sometimes using finite-frequency kernels. We simulated exact seismograms in 1-D and 3-D elastic models of the mantle. In a second simulation, we added topography to the discontinuities. We compared the waveforms obtained with and without topography by cross correlation of the SS precursors. Since we did not add noise, the precursors are visible in individual seismograms without the need of stacking. The resulting time anomalies were then converted into topographic variations and compared to the original topographic models. Based on the correlation between initial and inferred models, and provided that ray coverage is good, we found that linearised ray theory gives a relatively good idea on the location of the uplifts and depressions of the discontinuities. It seriously underestimates the amplitude of the topographic variations by a factor ranging between 2 and 7. Real data depend on the 3-D elastic structure and the topography. All studies to date correct for the 3-D elastic effects assuming that the traveltimes can be linearly decomposed into a structure and a discontinuity part. We found a strong non-linearity in this decomposition which cannot be modelled without a fully non-linear inversion for elastic structure and discontinuities simultaneously.

  10. Oxide induced corrosion on the welded stainless steels SS 2352 and 2353

    International Nuclear Information System (INIS)

    The pitting corrosion properties have been investigated in welded and unwelded condition by polarization tests in sodium chloride solutions. The two steels were TIG welded without adding welding material and as shielding on the bottom side argon gas containing 2, 26 or 99 ppm oxygen was used. In some tests low breakthrough potentials were received, without discovering any pitting corrosion in the specimen surfaces. The unwelded SS 2352 steel had a critical (lowest) pitting temperature (CPT) of 5 degrees C in the more concentrated solution. For the same steel with weld pitting corrosion was obtained at 5 degrees C, which was the lowest temperature for the tests. Thus the CPT value was lower than 5 degrees C, but by looking at the pitting corrosion potentials the following conclusion could be drawn: Welding with higher oxygen content in the shielding gas implied lower pitting corrosion resistance. For the SS 2353 steel the CPT values were 25 and 27.5 degrees C for material without weld, in contact with the more concentrated and the more dilute solution respectively. Welded material was all through more sensitive to pitting corrosion, and the CPT values were 15-17.5, 15 and 5-10 degrees C for welded areas which had been gas shielded with argon containing 2, 26 and 99 ppm oxygen respectively. The result thus showed that welding with shielding gas containing maximum about 30 ppm oxygen does not substantially affect the pitting corrosion properties. Post treatment of the welding areas increased the pitting corrosion resistance. Acid pickling implied the highest pitting corrosion resistance with 15 degrees C as CPT value for the 2353 steel in the more concentrated solution. Steel brushing implied an obvious increase to the pitting corrosion resistance compared to untreated weld areas and the same statement could be done for sand blasted surfaces. (10 refs., 16 tabs., 11 figs.)

  11. Investigating the presence of predatory bacteria on algal bloom samples using a T6SS gene marker.

    Science.gov (United States)

    Hendricks, J.; Sison-Mangus, M.; Mehic, S.; McMahon, E.

    2015-12-01

    Predation is considered to be a major driving force in evolution and ecology, which has been observed affecting individual organisms, communities, and entire ecosystems. The type VI secretion system (T6SS) is an intermembranal protein complex identified in certain bacteria, which appears to have evolved strictly as a mechanism of predation. The effects of bacteria on phytoplankton physiology are still understudied, however, studies have shown that the interactions between bacteria that inhabit the phycosphere of phytoplankton can possibly result in coevolution of native host and microbiota. It is unclear if bacteria can prey upon other bacteria to gain advantages during periods of high phytoplankton density. Here, we investigate the predatory interactions between bacteria and analyze environmental samples for the presence of predatory bacterial genes in an effort to understand bacteria-bacteria and phytoplankton interactions during algal blooms. DNA were extracted from bacterial samples collected weekly from size-fractionated samples using 3.0 um and 0.2 um membrane filters at the Santa Cruz wharf. PCR amplification and gel visualization for the presence of T6SS gene was carried out on bloom and non-bloom samples. Moreover, we carried out a lab- based experiment to observe bacteria-bacteria interaction that may hint for the presence of predatory behavior between bacterial taxa. We observed what appeared to be a predatory biofilm formation between certain bacterial species. These bacteria, however, did not contain the T6SS genes. On the contrary the T6SS gene was discovered in some of the bloom samples gathered from the Santa Cruz wharf. It is still unclear if the predatory mechanisms facilitate the abundance of certain groups of bacteria that contain the T6SS genes during algal blooms, but our evidence suggest that bacterial predation through T6SS mechanism is present during bloom events.

  12. Elevated expression of KiSS-1 in placenta of Chinese women with early-onset preeclampsia.

    Directory of Open Access Journals (Sweden)

    Chong Qiao

    Full Text Available Preeclampsia (PE is a heterogeneous syndrome affecting 2% to 8% of all pregnancies and is the world's leading cause of fetal and maternal morbidity and mortality. In many cases of PE, shallow trophoblast invasion results in inappropriate maternal spiral artery remodeling and impaired placental function. Multiple genes have been implicated in trophoblast invasion, among which are KiSS-1 and GPR54. The gene product of KiSS-1 is metastin, which is a ligand for the receptor GPR54. Both metastin and GPR54 are expressed in the placenta of normal pregnancy and have been implicated in modulating trophoblast invasion through inhibiting migration of trophoblast cells. We have previously reported that the expression level of KiSS-1 was higher in trophoblasts from women with preeclampsia as compared to normal controls. Here, using quantitative RT-PCR, Western blot analysis and immunohistochemistry, we extend our analysis to demonstrate that elevated KiSS-1 expression occurs only in early-onset preeclampsia (ePE and not late-onset preeclampsia (lPE. However, no difference in the expression levels of GPR54 is observed between ePE, lPE, and normal controls. Further, we show that KiSS-1 expression is also increased in placenta of intrauterine death and birth asphyxia in comparison to normal newborns of ePE and lPE. Our findings suggest that aberrant upregulation of KiSS-1 expression may contribute to the underlying mechanism of ePE as well as birth asphyxia.

  13. Study of diffusion bonding of WL10 to SS joining with and without titanium interlayer using thermo mechanical simulator

    International Nuclear Information System (INIS)

    The sound joining of Tungsten alloy (W+1%LaO3) material with Stainless Steel material (SS316L) is demanded for He-cooled divertor for demo like Tokamak in near future application. The life of the divertor finger can be assessed by quality of the joining between two the materials. Due to significantly large difference of thermal mismatch, the joining of WL10 and SS material is appeared to be difficult one. The Helium cooled modular divertor is expected to face with the heat load of 10MW/m2 with operation temperature at W tile ∼ 1700°C and 700°C for WL10 to SS region (thimble). There are possible ways for joining of these two materials such as vacuum brazing route, diffusion bonding route etc. In this paper, diffusion bonding route was followed to prepare the joining of the WL10 material and SS material using Thermo mechanical simulator machine (Gleeble 3800). Diffusion bonding of WL10 and SS material has been carried out at various temperatures 800 °C, 850°C, 900 °C, 950 °C and 1000 °C with uniaxial pressure 5 MPa to 20 MPa with the hold time of 15 Min and 30 Min with and without titanium interlayer. The joint specimens have been investigated by NDT (UT by immersion probe), microstructural examination and hardness measurement. In this paper, the methodology for the preparation of diffusion bonding and the optimization of the diffusion bonding parameters for the joining of WL10 and SS material will be discussed. (author)

  14. Effect of nitrogen on high temperature mechanical properties of type 316LN SS for fast reactor applications

    International Nuclear Information System (INIS)

    Long term creep, low cycle fatigue and creep-fatigue interaction properties as well as compatibility with liquid sodium coolant, govern the choice of materials for out-of-core structural components of sodium cooled fast reactors (SFRs). 316 L(N) SS containing 0.07 Wt.% nitrogen is the current preferred material for all the high temperature structural components of SFRs. For the prototype Fast Breeder Reactor also, which is nearing commissioning at Kalpakkam, 316 LN (SS) has been used for all the major high temperature structural components. It is proposed to design future SFRs for a life of at least 60 years. Therefore materials with higher creep and low cycle fatigue strength are required. Increasing the nitrogen content in 316L(N) SS is one of the approaches being pursued at Indira Gandhi Centre for Atomic Research (IGCAR) to develop a SFR structural material suitable for 60 years design life. Towards this, a strong R and D programme is currently underway at IGCAR. The effect of nitrogen in the range of 0.07 to 0.22 Wt.% on the tensile creep and low cycle fatigue behavior of 316 LN SS has been extensively investigated. Both tensile and creep strength were found to increase with increase in nitrogen content. High temperature low cycle fatigue life was found to peak in 316LN SS containing 0.14 Wt.%. The paper discusses the monotonic and cyclic deformation and fracture mechanisms by which nitrogen improves the creep and low cycle fatigue properties of 316 LN SS. (author)

  15. Kirjallisuuskatsaus sairaanhoitajan voimaantumista edistävistä tekijöistä gerontologisessa hoitotyössä

    OpenAIRE

    Ahonala, Johanna

    2012-01-01

    Opinnäytetyön tarkoituksena oli selvittää, mitä gerontologinen hoitotyö on kirjallisuuden mukaan, sekä millaisia ovat sairaanhoitajien voimaantumista edistävät tekijät gerontologisessa hoitotyössä. Aineisto koostui systemaattisen kirjallisuuskatsauksen menetelmällä hankituista tutkimuksista sekä tutkimusartikkeleista (n=26). Tämän kirjallisuuskatsauksen tulosten mukaan gerontologisessa hoitotyössä korostuvat eettiset periaatteet, ihmisarvon kunnioittaminen sekä inhimillinen hoito. Vallan...

  16. Laadun- ja riskienhallinta johtamisjärjestelmässä ISO 9001:2015 ja ISO 31000:2009 mukaisesti

    OpenAIRE

    Reinvall, Niko; Karhunen, Joonas

    2015-01-01

    Tämän opinnäytetyön tarkoituksena on tarkastella riskienhallinnan ja laadunhallintajärjestelmien suhdetta ISO 9001:2015 ja ISO 31000:2009-standardien viitekehyksessä, tutkimuskysymyksen ollessa ”miten ja miksi integroida ISO 9001 mukaista laadunhallintaa ja ISO 31000 mukaista riskienhallintaa organisaation johtamisjärjestelmään”. Lisäksi työssä tarkastellaan standardien välisiä yhtymäkohtia ja riskienhallinnan merkitystä ISO 9001:2015 mukaisessa laadunhallintajärjestelmässä. Työn tilaaja...

  17. Toimintaterapia maahanmuuttajatyössä : toimintaterapeuttien näkemyksiä työn haasteista ja mahdollisuuksista

    OpenAIRE

    Lappi, Salla

    2015-01-01

    Opinnäytetyössä tarkasteltiin, mitä toimintaterapia on maahanmuuttajatyössä ja minkälaisia haasteita tai mahdollisuuksia siihen suomalaisten toimintaterapeuttien näkemysten perusteella liittyy. Opinnäytetyön yhteistyökumppanina oli Tampereen kaupungin psykiatrian poliklinikka maahanmuuttajille. Yhteistyökumppanin lisäksi työn tuloksia voivat hyödyntää maahanmuuttajien parissa työskentelevät toimintaterapeutit, toimintaterapian opiskelijat ja opettajat sekä kaikki maahanmuuttajatyöstä ja sen k...

  18. Sosiaalipedagogisen hevostoiminnan tunnettavuus ja käyttö sosiaalisen kuntoutuksen keinona Jyväskylässä

    OpenAIRE

    Puustinen, Mari

    2015-01-01

    Hippolis- hevosalan osaamiskeskus ry:n päätehtävänä on toimia valtakunnallisena hevosalan yhteistyöorganisaationa. Hippolis osallistuu erilaisiin hevosalan yhteis-työprojekteihin, joiden aiheita ovat mm. hevosten käyttö hyvinvointipalveluissa ja hevonen kuntouttajana. Opinnäytetyön tavoitteena oli kartoittaa sosiaalipedagogisen hevostoiminnan tun-nettavuutta ja käyttöä Jyväskylässä. Sosiaalipedagoginen hevostoiminta on talliym-päristössä toteutettava sosiaalisen kuntoutuksen muoto. Se voi...

  19. Oppisopimuskoulutuksen kehittäminen sosiaali- ja terveysalan perustutkinnossa : – Työssä oppimisverkosto mahdollisuutena

    OpenAIRE

    Manninen, Mirva

    2012-01-01

    Opinnäytetyön aiheena on sosiaali- ja terveysalan perustutkinnon oppisopimuskoulutuksen kehittäminen. Tavoitteena on laatia toimintamalli sosiaali- ja terveysalan perustutkinnon oppisopimuskoulutuksen järjestämiseksi Kainuussa. Toimintamallin avulla tavoitellaan työssä oppimisen toteuttamista joustavasti siten, että opiskelijoille mahdollistuu sekä riittävän ammattitaidon saavuttaminen että palkan saaminen työssä oppimisen ajalta. Opinnäytetyön toimeksiantajana on Kainuun ammattiopisto –liike...

  20. Unity-pelimoottorin hyödyntäminen CAVE-järjestelmässä

    OpenAIRE

    Jussila, Asmo

    2013-01-01

    Opinnäytetyö toteutettiin Seinäjoen ammattikorkeakoulun Tekniikan yksikön virtuaalilaboratorion CAVE-tilaan. Työn tarkoituksena oli uudistaa CAVE:ssa käytössä olevia vanhahtavia virtuaalitodellisuusohjelmia. Työssä selvitettiin kolmen eri pelimoottorin soveltuvuutta CAVE-järjestelmään. Työn pelimoottorivertailuun valittiin suositut ja ilmaiseksi saatavilla olevat Cryengine 3 SDK, Unreal Development Kit sekä Unity-pelimoottorit. Pelimoottoreita vertailtiin keskenään graafisten ominaisuuksi...

  1. Mediator proteins orchestrate enzyme-ssDNA assembly during T4 recombination-dependent DNA replication and repair

    OpenAIRE

    Bleuit, Jill S.; Xu, Hang; Ma, Yujie; Wang, Tongsheng; Liu, Jie; Morrical, Scott W.

    2001-01-01

    Studies of recombination-dependent replication (RDR) in the T4 system have revealed the critical roles played by mediator proteins in the timely and productive loading of specific enzymes onto single-stranded DNA (ssDNA) during phage RDR processes. The T4 recombination mediator protein, uvsY, is necessary for the proper assembly of the T4 presynaptic filament (uvsX recombinase cooperatively bound to ssDNA), leading to the recombination-primed initiation of leading strand DNA synthesis. In the...

  2. Jatkuva uudistuminen ja esimiestyö : Menetelmiä jatkuvan uudistumisen tukemiseen esimiestyössä

    OpenAIRE

    Parhamaa, Tommi

    2015-01-01

    Tämän opinnäytetyön tarkoituksena oli tutkia jatkuvaa uudistumista kohdeorganisaation myynnin esimiestyössä. Tätä tutkittiin opinnäytetyössä Pentti Sydänmaanlakan jatkuvan uudistumisen elementit -mallia soveltaen. Mallissa jatkuva uudistuminen on jaettu neljään aihepiiriin: tiedon johtaminen, osaamisen johtaminen, suorituksen johtaminen sekä luovuuden ja innovatiivisuuden johtaminen. Näiden neljän elementin kautta organisaatio pystyy vaikuttamaan tehokkaasti jatkuvaan uudistumiseensa. Työ...

  3. Effect of zinc and copper additions on catalytic response of noble metal alloyed 304 SS in high temperature water

    International Nuclear Information System (INIS)

    The effect of zinc (Zn) and copper (Cu) additions on the catalytic behavior of noble metal alloyed 304 stainless steel (SS) in 288 C water understoichiometric excess hydrogen was studied. It was observed that an increase in the Zn or Cu content of the water increased the electrochemical corrosion potential (ECP) of noble metal alloyed 304 SS by ∼ 30 to 50 mV and decreased the recombination efficiency of oxygen (O2)and hydrogen (H2) by ∼ 10%. The change in the ECP and recombination rate was correlated with incorporation of zinc and copper in the oxide film, which, by covering catalytic sites, would alter the redox reaction rate

  4. Kuvista musiikiksi : jäsennetty kuvien käyttö 5-vuotiaiden musiikkileikkikouluryhmässä

    OpenAIRE

    Aaltonen, Linda

    2013-01-01

    Opinnäytetyössäni tutkin sitä, miten jäsennetty kuvien käyttö 5-vuotiaiden musiikkileikkikouluryhmässä vaikuttaa lasten keskittymiseen tunnilla. Lisäksi halusin selvittää, miten lapset itse kokivat tunnin ennakoitavuuden. Tutkimuskertoja oli kolme. Ensimmäisellä tunnilla en käyttänyt kuvia tunnin jäsentämiseen, ja kahdella jälkimmäisellä kerralla jäsensin tunnin viiden kuvan kuvasarjalla. Ensimmäisen ja viimeisen kerran lopuksi haastattelin lapsia saadakseni selville heidän mielipiteensä ...

  5. Corrosion, haemocompatibility and bacterial adhesion behaviour of TiZrN-coated 316L SS for bioimplants

    Indian Academy of Sciences (India)

    Gobi Saravanan Kaliaraj; Vinita Vishwakarma; Ananthakumar Ramadoss; D Ramachandran; Arul Maximus Rabel

    2015-08-01

    TiZrN coating was deposited on 316L stainless steel (SS) by the reactive magnetron co-sputtering technique. Cubic phase of TiZrN with uniform surface morphology was observed by X-ray diffraction and atomic force microscopy. Bacterial adhesion, haemocompatibility and corrosion behaviour of TiZrN coating were examined in order to evaluate the coating’s compatibility for ideal implant. Results revealed that TiZrN coatings exhibited less bacterial attachment against Staphylococcus aureus and Escherichia coli bacteria, negligible platelets activation and superior corrosion resistance than the uncoated 316L SS.

  6. Kulttuurierojen vaikutus yrityksen ja sidosryhmien välisessä yhteistyössä patentointialan yrityksessä

    OpenAIRE

    Saaristo, Anne

    2012-01-01

    Kulttuurieroja voidaan luokitella esimerkiksi alueen, uskonnon tai sukupolven mukaan. Maiden välisiä kulttuurieroja voidaan myös vertailla ja havainnollistaa kulttuuriulottuvuuksien avulla. Kulttuurien välisten erojen ymmärtäminen ja niiden huomioon ottaminen toiminnassa ja viestinnässä ovat oleellisia asioita kulttuuriosaamisessa, jota kansainvälistyvässä maailmassa arvostetaan yhä enemmän. Tämän opinnäytetyön tavoitteena oli tuottaa selvitys toimeksiantajayrityksen työntekijöiden mielip...

  7. Digitaalisen medianäytön hyödyntäminen markkinointiviestinnässä : case: Fazer

    OpenAIRE

    Lauanne, Marja

    2012-01-01

    Digitaalisuus on tuonut reaaliaikaisen viestinnän osaksi jokapäiväistä elämäämme. Tässä opinnäytetyössä tutkitaan yrityksen markkinointiviestintää ja digitaalisuuden viestintään tuomia muutoksia. Asiaa tarkastellaan markkinointiviestinnän suunnitteluprosessin kautta. Tutkitaan, kuinka markkinointiviestintäsuunnitelma rakennetaan, mitkä tekijät vaikuttava valintoihin prosessin sisällä ja kuinka saavutettuja tuloksia mitataan. Työn edetessä syvennytään tutkimaan digitaalista medianäyttöä ja s...

  8. Facebook-yhteisöpalvelun hyödyntäminen yrityksen markkinointiviestinnässä

    OpenAIRE

    Pajamäki, Ida

    2014-01-01

    Tämän opinnäytetyön tavoitteena oli selvittää, miksi yritysten olisi kannattavaa olla Facebook-yhteisöpalvelussa ja millaisia keinoja niillä on käytössään Facebook-palvelussa markkinoidessaan. Opinnäytetyössä rakennettiin työn toimeksiantajalle, Mainostoimisto Gurulle, oma Facebook-sivu. Guru on Turun ammattikorkeakoulun sisäinen mainostoimisto, jossa suunnitellaan kaikenlaista markkinointiviestintää opiskelijavoimin. Teoriaosassa käsiteltiin lyhyesti sosiaalista mediaa ja Facebook-palvel...

  9. LAPSEN MIELI VANHEMMAN MIELESSÄ : Vanhemmuuden kasvun tukeminen Järvenpään Sosiaalisairaalan perhekuntoutusyksikössä

    OpenAIRE

    Koskela, Leena

    2013-01-01

    TIIVISTELMÄ Leena, Koskela. Lapsen mieli vanhemman mielessä – Vanhemmuuden kasvun tukeminen Järvenpään Sosiaalisairaalan perhekuntoutusyksikössä. Syksy 2013, 126 sivua, 3 liitettä. Diakonia-ammattikorkeakoulu, Sosiaalialan koulutusohjelma, Diakonisen sosiaalityön suuntautumisvaihtoehto, sosionomi (AMK) + diakonin virkakelpoisuus. Yhä useampi lapsi altistuu raskaudenaikaiselle päihteidenkäytölle ja elää päihteiden värittämässä maailmassa. Päihteidenkäyttö vaikuttaa heikentävästi va...

  10. Rock-ravintolatoiminta : elävää rock-musiikkia ravintolaympäristössä

    OpenAIRE

    Sipilä, Esa; Väyliö, Jari

    2006-01-01

    Työn tavoitteena oli tutkia rock-ravintolatoimintaa ja elävää rock-musiikkia ravintolaympäristössä ravintolan, artistin ja asiakkaan näkökulmasta. Tutkimuksessa pyrittiin selvittämään rock-ravintolayrittämisen toimintatapoja ja kartoittamaan alan tämän hetkistä tilaa. Työssä tutustuttiin syvemmin suomalaiseen rock-ravintolatoimintaan yhtenä ravintolaelinkeinon haarana. Tutkimuksen aineisto kerättiin lomakehaastattelujen, teemahaastattelujen, osallistuvan havainnoinnin sekä kyselyjen avulla. T...

  11. Työssäjaksaminen työpaikan muutostilanteessa : ensihoidon näkökulma

    OpenAIRE

    Kivelä, Santtu; Nyman, Laura; Venhomaa, Jari

    2015-01-01

    Muutoksesta voidaan erottaa neljä eri tasoa; yksilön, työn, strategian- ja kulttuurin taso. Muutoksen tärkein osa on yksilön muutos, koska ilman yksilön muutosta muutosprosessi ei pääse liikkeelle (Erämetsä 2003, 193-198). Työntekijät odottavat tulevansa kuulluksi työpaikan muutostilanteissa. Tässä tutkimuksessa selvitettiin työssäjaksamista työpaikan muutostilanteessa ensihoidon piirissä työskentelevien työntekijöiden avulla. Tutkimuksen tarkoituksena on kartoittaa muutoksen vaikutusta ...

  12. Ectopic overexpression of SsCBF1, a CRT/DRE-binding factor from the nightshade plant Solanum lycopersicoides, confers freezing and salt tolerance in transgenic Arabidopsis.

    Directory of Open Access Journals (Sweden)

    Lili Zhang

    Full Text Available The C-repeat (CRT/dehydration-responsive element (DRE binding factor (CBF/DREB1 transcription factors play a key role in cold response. However, the detailed roles of many plant CBFs are far from fully understood. A CBF gene (SsCBF1 was isolated from the cold-hardy plant Solanum lycopersicoides. A subcellular localization study using GFP fusion protein indicated that SsCBF1 is localized in the nucleus. We delimited the SsCBF1 transcriptional activation domain to the C-terminal segment comprising amino acid residues 193-228 (SsCBF1(193-228. The expression of SsCBF1 could be dramatically induced by cold, drought and high salinity. Transactivation assays in tobacco leaves revealed that SsCBF1 could specifically bind to the CRT cis-elements in vivo to activate the expression of downstream reporter genes. The ectopic overexpression of SsCBF1 conferred increased freezing and high-salinity tolerance and late flowering phenotype to transgenic Arabidopsis. RNA-sequencing data exhibited that a set of cold and salt stress responsive genes were up-regulated in transgenic Arabidopsis. Our results suggest that SsCBF1 behaves as a typical CBF to contribute to plant freezing tolerance. Increased resistance to high-salinity and late flowering phenotype derived from SsCBF1 OE lines lend more credence to the hypothesis that plant CBFs participate in diverse physiological and biochemical processes related to adverse conditions.

  13. MECHANICAL PROPERTY RESEARCH OF sPS/PET/SsPS/MTA COMPOSITES%sPS/PET/SsPS/MTA复合材料的力学性能研究

    Institute of Scientific and Technical Information of China (English)

    王进; 祝方明; 林尚安

    2002-01-01

    用保持正常熔体插层法制备了新型的间规聚苯乙烯(sPS)/聚对苯二甲酸乙二酯(PET)/磺化间规聚苯乙烯(SsPS-H)及其锌盐(SsPS-Zn)/11-氨基酸改性蒙脱土(MTA)复合材料,该材料的综合力学性能较未加MTA材料显著提高.加入2份MTA时,sPS/PET/SsPS-H/MTA(质量比为85/15/4/2)和sPS/PET/SsPS-Zn/MTA(质量比为85/15/2/2)复合材料的冲击强度达到最大值,分别为15.6 kJ/m2和14.7 kJ/m2,约是纯sPS的3.5倍,是未加MTA材料的1.5倍.拉伸强度和弯曲强度比未填充材料都提高了约30 MPa.

  14. The pathogenicity factor HrpF interacts with HrpA and HrpG to modulate type III secretion system (T3SS) function and t3ss expression in Pseudomonas syringae pv. averrhoi.

    Science.gov (United States)

    Huang, Yi-Chiao; Lin, Yuan-Chuen; Wei, Chia-Fong; Deng, Wen-Ling; Huang, Hsiou-Chen

    2016-09-01

    To ensure the optimal infectivity on contact with host cells, pathogenic Pseudomonas syringae has evolved a complex mechanism to control the expression and construction of the functional type III secretion system (T3SS) that serves as a dominant pathogenicity factor. In this study, we showed that the hrpF gene of P. syringae pv. averrhoi, which is located upstream of hrpG, encodes a T3SS-dependent secreted/translocated protein. Mutation of hrpF leads to the loss of bacterial ability on elicitation of disease symptoms in the host and a hypersensitive response in non-host plants, and the secretion or translocation of the tested T3SS substrates into the bacterial milieu or plant cells. Moreover, overexpression of hrpF in the wild-type results in delayed HR and reduced t3ss expression. The results of protein-protein interactions demonstrate that HrpF interacts directly with HrpG and HrpA in vitro and in vivo, and protein stability assays reveal that HrpF assists HrpA stability in the bacterial cytoplasm, which is reduced by a single amino acid substitution at the 67th lysine residue of HrpF with alanine. Taken together, the data presented here suggest that HrpF has two roles in the assembly of a functional T3SS: one by acting as a negative regulator, possibly involved in the HrpSVG regulation circuit via binding to HrpG, and the other by stabilizing HrpA in the bacterial cytoplasm via HrpF-HrpA interaction prior to the secretion and formation of Hrp pilus on the bacterial surface. PMID:26638129

  15. Periodicity Analysis of X-ray Light Curves of SS 433

    Science.gov (United States)

    Wang, J. Y.; Lu, X. L.; Zhao, Q. W.; Dong, D. Q.; Lao, B. Q.; Lu, Y.; Wei, Y. H.; Wu, X. C.; An, T.

    2016-03-01

    SS 433 is the only X-ray binary to date that was detected to have a pair of well-collimated jets, and its orbital period, super orbital period, and nutation period were all detected at the same time. The study on the periodic X-ray variabilities is helpful for understanding its dynamic process of the central engine and the correlation with other bands. In the present paper, two time series analysis techniques, Lomb-Scargle periodogram and weighted wavelet Z-transform, are employed to search for the periodicities from the Swift/BAT (Burst Alert Telescope)(15--50 keV) and RXTE/ASM (Rossi X-Ray Timing Explorer/All-Sky Monitor)(1.5--3, 3--5 and 5--12 keV) light curves of SS 433, and the Monte Carlo simulation is performed. For the 15--50 keV energy band, five significant periodic signals are detected, which are P_1(˜6.29 d), P_2 (˜6.54 d), P_3 (˜13.08 d), P_4 (˜81.50 d), and P_5 (˜162.30 d). For the 3--5 and 5--12 keV energy bands, periodic signals P_3 (˜13 d) and P_5 (˜162 d) are detected in both energy bands. However, for the 1.5--3 keV energy band, no significant periodic signal is detected. P_5 has the strongest periodic signal in the power spectrum for all the energy bands of 3--5, 5--12, and 15--50 keV, and it is consistent with that obtained by previous study in optical band. Further, due to the existence of relativistic radio jets, the X-ray and optical band variability of P_5 (˜162 d) is probably related to the precession of the relativistic jets. High coherence between X-ray and optical light curves may also imply that the X-ray and optical emissions are of the same physical origin. P_3 shows a good agreement with the orbital period (˜13.07 d) first obtained by previous study, and P_2 and P_4 are the high frequency harmonic components of P_3 and P_5, respectively. P_1 is detected from the power spectrum of 15--50 keV energy band only, and it is consistent with the systematic nutation period. As the power of energy band decreases (from hard X-ray to

  16. Dynamic rockfall risk assessment along the SS113 coastal road (Northern Sicily)

    Science.gov (United States)

    Mastrolembo, V. Brunella; Penna, Ivanna; Voumard, Jérémie; Jaboyedoff, Michel

    2016-04-01

    Rockfalls are natural hazards that usually affect only small areas. However, due to the big amount of material that can be moved and the associated kinetic energy they can cost serious damages to infrastructures and people. Even fairly small rockfall fragments are a significant hazard if deposited on a highway or along a rail-road track since they are not easily detected and can cause accidents or derailments. Rockfalls can also cause the closure of streets resulting in long term indirect economic losses due to transportation delays as well as to the impact on the commercial and tourist activities. In letterature there are numerous examples of rockfall risk assessments along transportation corridors, most of them are based on the use of standard risk estimation methods. The latters are static approaches founded on a macroscopic view of road traffic, assumed as uniform in space and time, thus characterized by average values of parameters. Lately, a new dynamic approach has been developed within the 'risk analysis group' at the University of Lausanne (Voumard, 2013). It consists of a kinematic interpretation of road traffic where vehicles are parametrized as single entities with different characteristics, speed, dimensions and behaviour. We apply this new approach to estimate the dynamic risk due to rockfall occurrence on the SS113 national road running along the northern coast of Sicily. In this work we focus our attention on a ≈10 km section along which the SS113 road and a railway connect all the costal villages going through very steep cliffs and very close to the sea with evident problems of erosion and maintenance. The area is a tourist destination and many hotels and facilities are found along the road. Moreover the area was already hit in the past by numerous rockfalls resulting in the closure of the road for periods running from a few days up to a few years with big direct and indirect damages to the local socio-economic activities. In order to achieve a

  17. Diagnosis of gallbladder cancer by imaging techniques. Problems, limitations, and their explanations, especially with ss invasive cancer

    Energy Technology Data Exchange (ETDEWEB)

    Tomita, Masao; Onoyama, Hirohiko; Sako, Tatsuo; Ajiki, Tetsuo; Ohara, Sigetsugu; Yamazaki, Iwao; Yamamoto, Masahiro; Saito, Yoichi (Kobe Univ. (Japan). School of Medicine)

    1994-11-01

    The preoperative diagnosis of less than ss depth gallbladder cancer is difficult. Its preoperative diagnosis rate was low (27.5%), and even lower with the presence of concomitant gallstone. In the latter case,the diagnosis rate was particularly low when the stones diameter was greater than 1 cm. Gallstones are frequently associated with macromorphologically invasive type of gallbladder cancer and this may be the reason for the lower diagnostic rate. We compared preoperative diagnosis rate of ultrasonography (US), computed tomography (CT) and drip infusion cholecystography (DIC) for each invasion depth. In US, the preoperative diagnosis rate for m depth invasion was 6/16 (37.5%), pm depth was 1/13 (7.7%), and ss depth was 14/41 (34.1%). In CT, its rate for m depth was 3/11 (27.3%), pm depth was 1/10 (10.0%), and ss depth was 11/37 (29.7%). In DIC, its rate for m depth was 3/11 (27.3%), pm depth was 1/10 (10.0%), and ss was depth 1/23 (4.3%). None of the currently used imaging techniques were very accurate in diagnosing gallbladder cancer. Thus, during preoperative work up, if one discovers a gallbladder full of stones, stones of greater than 1 cm in diameter, thickened gallbladder wall, or a non visualized gallbladder with DIC, gallbladder cancer must be highly suspected. (author).

  18. Effects of heat treatments on microstructure changes in the interface of Cu/SS316L joint materials

    International Nuclear Information System (INIS)

    Precipitation and dispersion strengthened copper alloys joined with 316L austenitic stainless steel are expected to be heat sink materials in the first wall and divertor of International Thermonuclear Experimental Reactor (ITER) owing to the good thermal conductivity of Cu alloys. In the present study, the effects of heat treatment on microstructural stability in the interface of CuNiBe/SS316L and CuAl25/SS316L have been investigated. In the as-received CuNiBe/SS316L joints, voids were observed at the interface, and in the stainless steel side near the interface. But in the CuAl25/SS316L joints, voids were observed only in the Cu side near the interface. These voids would have a significant effect on the mechanical properties of joints. The results of annealing experiments showed that the microstructures in the interface of both types of joints were thermally stable during annealing at 573 and 673 K for 100 h

  19. Complete genome sequence of SS52, a strain of Escherichia coli O157:H7 recovered from supershedder cattle

    Science.gov (United States)

    Shiga toxin-producing Escherichia coli O157:H7 cause foodborne infections and cattle are the primary reservoir. Some animals, known as supershedders, excrete orders of magnitude more E. coli O157:H7 in the feces than normal. We here report the complete genome sequence of the SS52 supershedder stra...

  20. Molecular visualization of the yeast Dmc1 protein ring and Dmc1-ssDNA nucleoprotein complex.

    Science.gov (United States)

    Chang, Yuan-Chih; Lo, Yu-Hui; Lee, Ming-Hui; Leng, Chih-Hsiang; Hu, Su-Ming; Chang, Chia-Seng; Wang, Ting-Fang

    2005-04-26

    Saccharomyces cerevisiae Dmc1, a meiosis-specific homologue of RecA, catalyzes homologous pairing and strand exchange during meiotic DNA recombination. The purified budding yeast Dmc1 (ScDmc1) protein exhibits much weaker recombinase activity in vitro as compared to that of the Escherichia coli RecA protein. Using atomic force microscopy (AFM) with carbon nanotube tips, we found ScDmc1 forms rings with an external diameter of 18 nm and a central cavity of 4 nm. In the presence of single-stranded DNA (ssDNA), the majority of the ScDmc1 protein (90%) bound DNA as protein rings; only a small faction (10%) was able to form filamentous structure. In contrast, nearly all RecA proteins form fine helical nucleoprotein filaments with ssDNA under identical conditions. RecA-mediated recombinase activity is initiated through the nucleation of RecA onto ssDNA to form helical nucleoprotein filaments. Our results support the notion that ScDmc1 becomes catalytically active only when it forms a helical nucleoprotein filament with ssDNA. PMID:15835894