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Sample records for blazei ss heinemann

  1. Cytotoxicity and genotoxicity of Agaricus blazei methanolic extract fractions assessed using gene and chromosomal mutation assays

    OpenAIRE

    Marilanda Ferreira Bellini; Leonardo Neves Cabrioti; Ana Paula Terezan; Berenice Quinzani Jordão; Lúcia Regina Ribeiro; Mário Sérgio Mantovani

    2008-01-01

    Functional food investigations have demonstrated the presence of substances that could be beneficial to human health when consumed. However, the toxic effects of some substances contained in foods have been determined. Reported medicinal and nutritive properties have led to the extensive commercialization of the basidiomycete fungi Agaricus blazei Murrill (sensu Heinemann), also known as Agaricus brasiliensis Wasser et al., Agaricus subrufescens Peck or the Brazilian medical mushroom (BMM). D...

  2. Cytotoxicity and genotoxicity of Agaricus blazei methanolic extract fractions assessed using gene and chromosomal mutation assays

    Directory of Open Access Journals (Sweden)

    Marilanda Ferreira Bellini

    2008-01-01

    Full Text Available Functional food investigations have demonstrated the presence of substances that could be beneficial to human health when consumed. However, the toxic effects of some substances contained in foods have been determined. Reported medicinal and nutritive properties have led to the extensive commercialization of the basidiomycete fungi Agaricus blazei Murrill (sensu Heinemann, also known as Agaricus brasiliensis Wasser et al., Agaricus subrufescens Peck or the Brazilian medical mushroom (BMM. Different methanolic extract fractions (ME of this mushroom were submitted to the cytokinesis-block micronucleus (CBMN clastogenic assay and the hypoxanthine-guanine phosphoribosyl transferase locus (HGPRT assay for gene mutation, both using Chinese hamster ovary cells clone K1 (CHO-K1. The results suggest that all the fractions tested possess cytotoxic and mutagenic potential but no clastogenic effects. Further information is needed on the biochemical components of the A. blazei methanol fractions to identify any substances with cytotoxic and/or mutagenicity potential. These findings indicate that A. blazei methanolic extract should not be used due to their genotoxicity and care should be taken in the use of A. blazei by the general population until further biochemical characterization of this fungi is completed.

  3. 巴氏菇Agaricus blazei murrill栽培新法初步研究%PRELIMINARY STUDIES ON NEW METHOD FOR CULTIUATING AGARICUS BLAZEI MURRILL

    Institute of Scientific and Technical Information of China (English)

    杨旭

    2000-01-01

    A technique for cultivating agaricus blazei murrill is reported. The technique used bagasse, in stead of muck as material, and through the secondary fermention of cultivated material to cultivate.%本文报导了以苷蔗渣为原料,不用粪肥,经栽培料二次发酵生产agaricus blazei murrill技术.

  4. Cultivation of Agaricus blazei on Pleurotus spp. spent substrate

    Directory of Open Access Journals (Sweden)

    Regina Maria Miranda Gern

    2010-08-01

    Full Text Available The aim of this work was the use of Pleurotus ostreatus and Pleurotus sajor-caju for the previous lignocellulolytic decomposition of banana tree leaf straw and the further use of the degraded straw as substrate for the culture of Agaricus blazei. For optimising the production of A. blazei in terms of yield (Y% and biological efficiency (BE%, adjustments to the composition of the substrate were evaluated in a 2(5 experimental design. The following components were tested in relation to % of substrate dry mass: urea (1 and 10%, rice bran (10 or 20% or ammonium sulphate (0 or 10%, inoculum (10 or 20% and the casing material (subsoil or burned rice husks. The best results (79.71 Y% and 6.73 BE% were found when the substrate containing 10% of rice bran, without ammonium sulphate, inoculated with 20% and covered with subsoil was used.O cultivo de fungos comestíveis e medicinais utilizando resíduos da agroindústria vem se apresentando como uma alternativa econômica para o pequeno produtor rural, favorecendo a agricultura familiar do nordeste catarinense. Este trabalho avaliou o fungo Pleurotus para a decomposição lignocelulolítica de palha de folhas de bananeira e a utilização da palha residual como substrato para o cultivo de Agaricus blazei. Ajustes na composição do substrato residual de Pleurotus, tais como o tipo e a concentração da fonte de nitrogênio, a porcentagem de inóculo e a camada de cobertura, foram avaliadas. O substrato residual que mais favoreceu a produção de A. blazei em Eficiência Biológica (6,73%, Rendimento (79,71% e menor tempo para emissão do primeiro primórdio (27 dias foi o substrato residual de P. ostreatus inoculado com 20% de inóculo (ms, 10% de farelo de arroz (ms, sem sulfato de amônio e utilizando terra de subsolo como camada de cobertura.

  5. Agaricus blazei, supstrat za proizvodnju β-1,3-glukanaze s pomoću Trichoderma harzianum Rifai

    OpenAIRE

    Carneiro, Andréia A. J.; Giese, Ellen C.; Barbosa, Aneli M.; Gomes, Eleni; da Silva, Roberto

    2011-01-01

    Ekstrakt gljive Agaricus blazei (Agaricus brasiliensis) upotrijebljen je kao supstrat za proizvodnju β-1,3-glukanaze submerznim uzgojem plijesni Trichoderma harzianum Rifai. Centralno složeni 22 faktorijalni dizajn razvijen je primjenom ovih varijabla: vrijeme uzgoja (x1/dan) i koncentracija ekstrakta Agaricus blazei (x2/(g/L)), a rezultati su analizirani metodom odzivnih površina (RSM). Rezultati pokazuju da je koncentracija ekstrakta Agaricus blazei najvažnija varijabla za proizvodnju β-1,3...

  6. Ipomoea batatas and Agarics blazei ameliorate diabetic disorders with therapeutic antioxidant potential in streptozotocin-induced diabetic rats

    OpenAIRE

    Niwa, Atsuko; Tajiri, Takashi; Higashino, Hideaki

    2011-01-01

    Ipomoea batatas, Agaricus blazei and Smallanthus sonchifolius are known to favorably influence diabetes mellitus. To clarify their antidiabetic efficacy and hypoglycemic mechanisms, we treated streptozotocin-induced diabetic rats with daily oral feeding of powdered Ipomoea batatas (5 g kg−1 d−1), Agaricus blazei (1 g kg−1 d−1) or Smallanthus sonchifolius (4 g kg−1 d−1) for 2 months. Treatments with Ipomoea batatas or Agaricus blazei, but not Smallanthus sonchifolius, significantly suppressed ...

  7. IL-12 Production Induced by Agaricus blazei Fraction H (ABH) Involves Toll-like Receptor (TLR)

    OpenAIRE

    Kasai, H.; He, L. M.; Kawamura, M.; Yang, P. T.; Deng, X. W.; Munkanta, M.; Yamashita, A; H Terunuma; Hirama, M.; Horiuchi, I.; Natori, T.; Koga, T; Y. Amano; Yamaguchi, N.; Ito, M

    2004-01-01

    Agaricus blazei Murill is an edible fungus used in traditional medicine, which has various well-documented medicinal properties. In the present study, we investigated the effects of hemicellulase-derived mycelia extract (Agaricus blazei fraction H: ABH) on the immune system. First, we examined the cytokine-inducing activity of ABH on human peripheral mononuclear cells (PBMC). The results indicated that ABH induced expression of IL-12, a cytokine known to be a critical regulator of cellular im...

  8. Effect of different compounds on the induction of laccase production by Agaricus blazei.

    Science.gov (United States)

    Valle, J S; Vandenberghe, L P S; Oliveira, A C C; Tavares, M F; Linde, G A; Colauto, N B; Soccol, C R

    2015-01-01

    Laccases are polyphenol oxidases produced by many fungi and have many applications in textile, food and beverage, and pulp and paper industries. Laccase production can be induced using aromatic or phenolic compounds that mostly affect the transcription of laccase-encoding genes. In this study, we analyzed laccase and biomass production by Agaricus blazei in the presence of different concentrations of nitrogen, copper, and inducers such as pyrogallol, veratryl alcohol, xylidine, vanillin, guaiacol, and ethanol. Laccase production by A. blazei U2-4 reached 43.8 U/mL in the presence of 2.8 g/L nitrogen and 150 μM copper. However, addition of copper to the cultivation medium decreased biomass production. Different compounds differentially induced laccase production by A. blazei. Moreover, different concentrations of these inducers exerted different effects on laccase activity. Ethanol (1.0 mM), guaiacol (0.5 mM), and vanillin (0.5 mM) were the best inducers and increased laccase activity by 120% (A. blazei U2-2), 30% (A. blazei U2-3), and 9% (A. blazei U2-4), respectively. In contrast, pyrogallol and xylidine decreased laccase activity but increased biomass production. PMID:26634556

  9. Agaricus Blazei Hot Water Extract Shows Anti Quorum Sensing Activity in the Nosocomial Human PathogenPseudomonas Aeruginosa

    NARCIS (Netherlands)

    Sokovic, M.; Ciric, A.; Glamoclija, J.; Nicolic, M.; Griensven, van L.J.L.D.

    2014-01-01

    The edible mushroom Agaricus blazei Murill is known to induce protective immunomodulatory action against a variety of infectious diseases. In the present study we report potential anti-quorum sensing properties of A. blazei hot water extract. Quorum sensing (QS) plays an important role in virulence,

  10. Production flush of Agaricus blazei on Brazilian casing layers

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    Nelson Barros Colauto

    2011-06-01

    Full Text Available This study aimed to verify the biological efficiency and production flushes of Agaricus blazei strains on different casing layers during 90 cultivation days. Four casing layers were used: mixture of subsoil and charcoal (VCS, lime schist (LSC, São Paulo peat (SPP and Santa Catarina peat (SCP; and two genetically distant A. blazei strains. The fungus was grown in composted substratum and, after total colonization, a pasteurized casing layer was added over the substratum, and fructification was induced. Mushrooms were picked up daily when the basidiocarp veil was stretched, but before the lamella were exposed. The biological efficiency (BE was determined by the fresh basidiocarp mass divided by the substratum dry mass, expressed in percentage. The production flushes were also determined over time production. The BE and production flushes during 90 days were affected by the strains as well as by the casing layers. The ABL26 and LSC produced the best BE of 60.4%. Although VCS is the most used casing layer in Brazil, it is inferior to other casing layers, for all strains, throughout cultivation time. The strain, not the casing layer, is responsible for eventual variations of the average mushroom mass. In average, circa 50% of the mushroom production occurs around the first month, 30% in the second month, and 20% in third month. The casing layer water management depends on the casing layer type and the strain. Production flush responds better to water reposition, mainly with ABL26, and better porosity to LSC and SCP casing layers.

  11. Waffen-SS

    DEFF Research Database (Denmark)

    Christensen, Claus Bundgård

    Bogen analyserer SS-organisationens væbnede gren, Waffen-SS ud fra en række vinkler. De vigtigste er: 1) koblingen mellem Waffen-SS og resten af SS, 2) Waffen-SS som militær faktor i Det Tredje Rige, 3) Waffen-SS soldaternes politiske indoktrinering, 4) relationerne mellem de mange nationaliteter......, som tjente i Waffen-SS, 5) Waffen-SS andel i de nazistiske forbrydelser, 6) SS-veteranerne efter Anden Verdenskrig...

  12. Genetic characterization of isolates of the basidiomycete Agaricus blazei by RAPD

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    Colauto Nelson Barros

    2002-01-01

    Full Text Available The genetic divergence of five isolates of Agaricus blazei was determined based on RAPD data. Results indicate that there is little genetic variability among the commercialized strains and that RAPD is a feasible and low cost technique that can be used to characterize this fungus.

  13. Genetic characterization of isolates of the basidiomycete Agaricus blazei by RAPD

    OpenAIRE

    Colauto Nelson Barros; Dias Eustáquio Souza; Gimenes Marcos Aparecido; Eira Augusto Ferreira da

    2002-01-01

    The genetic divergence of five isolates of Agaricus blazei was determined based on RAPD data. Results indicate that there is little genetic variability among the commercialized strains and that RAPD is a feasible and low cost technique that can be used to characterize this fungus.

  14. Agaricus Blazei Hot Water Extract Shows Anti Quorum Sensing Activity in the Nosocomial Human Pathogen Pseudomonas Aeruginosa

    OpenAIRE

    Marina Soković; Ana Ćirić; Jasmina Glamočlija; Miloš Nikolić; Van Griensven, Leo J. L. D.

    2014-01-01

    The edible mushroom Agaricus blazei Murill is known to induce protective immunomodulatory action against a variety of infectious diseases. In the present study we report potential anti-quorum sensing properties of A. blazei hot water extract. Quorum sensing (QS) plays an important role in virulence, biofilm formation and survival of many pathogenic bacteria, including the Gram negative Pseudomonas aeruginosa, and is considered as a novel and promising target for anti-infectious agents. In thi...

  15. Chemical, enzymatic and cellular antioxidant activity studies of Agaricus blazei Murrill

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    RICARDO A. HAKIME-SILVA

    2013-09-01

    Full Text Available Mushrooms possess nutritional and medicinal properties that have long been used for human health preservation and that have been considered by researchers as possible sources of free radical scavengers. In this work, the antioxidant properties of water extracts from Agaricus blazei Murill, produced by maceration and decoction, are demonstrated in vitro. Resistance to oxidation is demonstrated through three mechanisms: i inhibition of enzymatic oxidative process, with 100% inhibition of HRP (horseradish peroxidase and MPO (myeloperoxidase; ii inhibition of cellular oxidative stress, with 80% inhibition of the oxidative burst of polymorphonuclear neutrophils (PMNs; and iii direct action over reactive species, with 62% and 87% suppression of HOCl and superoxide anion radical (O2• –, respectively. From the data, it was concluded that the aqueous extract of A. blazei has significant antioxidant activity, indicating its possible application for nutraceutical and medicinal purposes.

  16. The Medicinal Mushroom Agaricus blazei Murrill: Review of Literature and Pharmaco-Toxicological Problems

    OpenAIRE

    F.Firenzuoli; Gori, L.; Lombardo, G.

    2007-01-01

    Agaricus blazei Murrill (ABM) popularly known as ‘Cogumelo do Sol’ in Brazil, or ‘Himematsutake’ in Japan, is a mushroom native to Brazil, and widely cultivated in Japan for its medicinal uses, so it is now considered as one of the most important edible and culinary-medicinal biotechnological species. It was traditionally used to treat many common diseases like atherosclerosis, hepatitis, hyperlipidemia, diabetes, dermatitis and cancer. In vitro and in vivo ABM has shown immunomodulatory and ...

  17. The Medicinal Values of Culinary-Medicinal Royal Sun Mushroom (Agaricus blazei Murrill)

    OpenAIRE

    Hang Wang; Zhiming Fu; Chunchao Han

    2013-01-01

    Agaricus blazei Murrill (ABM), a mushroom native to Brazil, is a basidiomycete brown fungus, which is popularly known as “Cogumelo do Sol” in Brazil or “Himematsutake” in Japan, and there has been a prominent increase in the use of ABM for therapeutic and medicinal purposes. ABM is useful against a variety of diseases like cancer, tumor, chronic hepatitis, diabetes, atherosclerosis, hypercholesterolemia, and so on. In this review, we demonstrated various pharmacological effects of ABM, so tha...

  18. Agarol, an ergosterol derivative from Agaricus blazei, induces caspase-independent apoptosis in human cancer cells.

    Science.gov (United States)

    Shimizu, Takamitsu; Kawai, Junya; Ouchi, Kenji; Kikuchi, Haruhisa; Osima, Yoshiteru; Hidemi, Rikiishi

    2016-04-01

    Agaricus blazei (A. blazei) is a mushroom with many biological effects and active ingredients. We purified a tumoricidal substance from A. blazei, an ergosterol derivative, and named it 'Agarol'. Cytotoxic effects of Agarol were determined by the MTT assay using A549, MKN45, HSC-3, and HSC-4 human carcinoma cell lines treated with Agarol. Apoptosis was detected by flow cytometry analysis. Reactive oxygen species (ROS) levels and mitochondria membrane potential (∆ψm) were also determined by flow cytometry. Western blot analysis was used to quantify the expression of apoptosis-related proteins. Agarol predominantly induced apoptosis in two p53-wild cell lines (A549 and MKN45) compared to the other p53-mutant cell lines (HSC-3 and HSC-4). Further mechanistic studies revealed that induction of apoptosis is associated with increased generation of ROS, reduced ∆ψm, release of apoptosis-inducing factor (AIF) from the mitochondria to the cytosol, upregulation of Bax, and downregulation of Bcl-2. Caspase-3 activities did not increase, and z-VAD-fmk, a caspase inhibitor, did not inhibit the Agarol-induced apoptosis. These findings indicate that Agarol induces caspase-independent apoptosis in human carcinoma cells through a mitochondrial pathway. The in vivo anticancer activity of Agarol was confirmed in a xenograft murine model. This study suggests a molecular mechanism by which Agarol induces apoptosis in human carcinoma cells and indicates the potential use of Agarol as an anticancer agent. PMID:26893131

  19. Composto exaurido do cogumelo Agaricus blazei na dieta de frangos de corte Spent mushroom substrate of Agaricus blazei in broiler chicks diet

    Directory of Open Access Journals (Sweden)

    Alexandre Magno Batista Machado

    2007-08-01

    Full Text Available Avaliou-se o efeito da substituição de um antibiótico por diferentes níveis do composto exaurido do cogumelo (CEC Agaricus blazei sobre o desempenho de frangos de corte de 1 a 42 dias de idade. Foram avaliadas sete dietas, seis com adição de CEC (0,0; 0,2; 0,4; 0,6; 0,8 e 1,0% e uma com antibiótico (avilamicina 10 ppm. Foram utilizados 588 pintos de 1 dia de idade, distribuídos em um delineamento inteiramente casualizado, com seis repetições por dieta (três por sexo e 14 aves por unidade experimental. As aves que não receberam qualquer aditivo na dieta apresentaram os piores resultados de desempenho. Níveis de CEC superiores a 0,4% ocasionaram redução gradual no desempenho. O nível de 0,2% de CEC proporcionou o maior ganho de peso e o de 0,21%, a melhor conversão alimentar e o maior consumo de ração. À exceção do rendimento de peito e do rendimento de gordura abdominal, os aditivos não influenciaram os rendimentos de carcaça e dos cortes. O composto exaurido do cogumelo Agaricus blazei pode ser utilizado como aditivo alternativo ao antibiótico no nível de 0,2% da dieta, pois não prejudicou o desempenho produtivo e o rendimento de carcaça das aves.The effect of the substitution of an antibiotic by different levels of spent mushroom substrate (SMS of Agaricus blazei on the performance of broiler chicks from 1 to 42 days old was evaluated. Seven diets were evaluated, six with SMS addiction (0.0, 0.2, 0.4, 0.6, 0.8, and 1.0% and one with antibiotic (avilamycin 10 ppm. A total of 588 one day old chicks was allotted to a complete randomized design, with six replications per diet (three per sex and 14 birds per experimental unit. The birds fed diet with any additive showed the worst performance results. Levels of SMS above 0.4% caused gradual reduction in the performance. The level of 0.2% of SMS provided the highest value of weight gain and of 0.21% promoted the best feed conversion and the highest value of feed intake

  20. Analgesic Activity of a Glucan Polysaccharide Isolated from Agaricus blazei Murill

    OpenAIRE

    Maria Leônia C. Gonzaga; Menezes, Thiago M. F.; de Souza, José R. R.; Nágila M. P. S. Ricardo; Ana L. P. Freitas; Sandra de A. Soares

    2013-01-01

    A water soluble polysaccharide and its selective precipitated fractions (F1, F2, and F3) were isolated from hot water extract of fruiting bodies of Agaricus blazei Murril known as Agaricus brasiliensis to evaluate the analgesic activity of the materials in Swiss mice. The isolated material and its fractions were characterized by two-dimensional COSY and HMQC spectra as β-(1→6)-α-(1→4)-glucan-bound-protein polysaccharide constituted mainly by β-(1→6) glucan. The glucan-protein polysaccharide a...

  1. Study on Several Characteristics of Agaricus blazei Murril Strain J3 Irradiated by 60Co

    Institute of Scientific and Technical Information of China (English)

    WENG Bo-qi; JIANG Zhi-he; HUANG Ting-jun; CHEN Jian; ZHENG Wei-wen

    2003-01-01

    A new mutant strain J3 from Agaricus blazei Murril was obtained by 60Co irradiation. The yields of successive generations were increased more than 70% to compare with their original strain. The component analysis on amino acids and fatty acid illuminated that the nutrient value of strain J3 fruiting body was better than the original strain. The apparent nodule structure was found in the hyphea of J3 strain through the observation by scanning electron microscope. RAPD analysis showed the great difference of PCR fingerprints between J3 and its original strain. It is a promising mutant strain for further commercial development in the future.

  2. Coloração de núcleos de esporos e hifas do cogumelo Agaricus blazei Nucleus stain of spores and hifas Ofagaricus blazei

    Directory of Open Access Journals (Sweden)

    Cláudia Regina Gontijo Labory

    2003-04-01

    Full Text Available Com o objetivo de se estudar o comportamento nuclear durante o ciclo sexual do cogumelo Agaricus blazei , técnicas de coloração e a utilização de fluorocromos foram testadas, visando a uma padronização metodológica para estudos citogenéticos futuros. Das técnicas avaliadas neste trabalho (método de Feulgen, coloração com Giemsa e fluorescência-DAPI, a coloração com Giemsa e o fluorocromo DAPI apresentaram os melhores resultados para evidenciar núcleos. O corante Giemsa permitiu a visualização de compartimentos multinucleados. Com o DAPI, não foi possível a visualização dos septos, sendo necessária a utilização adicional de calcofluor, que tem a propriedade de corar a quitina que delimita os compartimentos das hifas.In order to study the nuclear behavior during the sexual cycle of Agaricus blazei , some coloration and fluorescent stain techniques were tested for a method standardization for fut ure cytogenetical works. Among the studied techniques (Schiff reactive, Giemsa, and fluorescent stain - DAPI, the Giemsa and fluorescent DAPI technique provided the best results. With Giemsa stain, it was possible to visualize multinucleate cell compartments. It was not possible to stain compartmental delimitation with DAPI stain, which was possible only with an additional fluorescent stain - calcofluor, which is specific for chitin.

  3. Agaricus Blazei Hot Water Extract Shows Anti Quorum Sensing Activity in the Nosocomial Human Pathogen Pseudomonas Aeruginosa

    Directory of Open Access Journals (Sweden)

    Marina Soković

    2014-04-01

    Full Text Available The edible mushroom Agaricus blazei Murill is known to induce protective immunomodulatory action against a variety of infectious diseases. In the present study we report potential anti-quorum sensing properties of A. blazei hot water extract. Quorum sensing (QS plays an important role in virulence, biofilm formation and survival of many pathogenic bacteria, including the Gram negative Pseudomonas aeruginosa, and is considered as a novel and promising target for anti-infectious agents. In this study, the effect of the sub-MICs of Agaricus blazei water extract on QS regulated virulence factors and biofilm formation was evaluated against P. aeruginosa PAO1. Sub-MIC concentrations of the extract which did not kill P. aeruginosa nor inhibited its growth, demonstrated a statistically significant reduction of virulence factors of P. aeruginosa, such as pyocyanin production, twitching and swimming motility. The biofilm forming capability of P. aeruginosa was also reduced in a concentration-dependent manner at sub-MIC values. Water extract of A. blazei is a promising source of antiquorum sensing and antibacterial compounds.

  4. Effects of extraction methods on the antioxidant activities of polysaccharides from Agaricus blazei Murrill.

    Science.gov (United States)

    Jia, Shaoyi; Li, Feng; Liu, Yong; Ren, Haitao; Gong, Guili; Wang, Yanyan; Wu, Songhai

    2013-11-01

    Five polysaccharides were obtained from Agaricus blazei Murrill (ABM) through different extraction methods including hot water extraction, single enzyme extraction (pectinase, cellulase or papain) and compound enzymes extraction (cellulase:pectinase:papain). Their characteristics such as the polysaccharide yield, polysaccharide content, protein content, infrared spectra were determined, and antioxidant activities were investigated on the basis of hydroxyl radical, DPPH free radical, ABTS free radical and reducing power. The results showed that five extracts exhibited antioxidant activities in a concentration-dependent manner. Compared with other methods, the compound enzymes extraction method was found to present the highest polysaccharides yield (17.44%). Moreover, compound enzymes extracts exhibited the strongest reducing power and highest scavenging rates on hydroxyl radicals, DPPH radicals and ABTS radicals. On the contrary, hot water extraction method had the lowest polysaccharides yield of 11.95%, whose extracts also exhibited the lowest antioxidant activities. Overall, the available data obtained in vitro models suggested that ABM extracts were natural antioxidants and compound enzymes extraction was an appropriate, mild and effective extracting method for obtaining the polysaccharide extracts from Agaricus blazei Murrill (ABM).

  5. Genotoxic and antigenotoxic effects of organic extracts of mushroom Agaricus blazei Murrill on V79 cells

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    Guterres Zaira da Rosa

    2005-01-01

    Full Text Available Agaricus blazei Murrill, popularly known as the sun mushroom, is a native mushroom in SP, Brazil, that has been widely used in the treatment of cancer and many other pathologies in different parts of the world. A water-soluble protein-polysaccharide complex (1 -> 6beta-D-glucan has been isolated from its fruiting body that showed immune-modulation activity. From organic extracts, linoleic acid has been isolated and determined to be the main substance with antimutagenic activity. Using both the micronucleus (MN and comet (single cell microgel electrophoresis assays, this study determined the genotoxic and antigenotoxic potential of A. blazei (AB obtained from commercial sources or the following strains: a strains AB 97/29 (young and sporulated phases; b a mixture taken from AB 96/07, AB 96/09 and AB 97/11 strains; and c commercial mushrooms from Londrina, PR and Piedade, SP, designated as AB PR and AB SP, respectively. The extracts from these mushrooms were isolated in chloroform:methanol (3:1 and used in vitro at three different concentrations. V79 cells (Chinese hamster lung cells were exposed to the extracts under pre-, simultaneous and post-treatment conditions, combined with methyl methanesulfonate (MMS. Under the circumstances of this study, these organic extracts did not show any genotoxic or mutagenic effects, but did protect cells against the induction of micronuclei by MMS.

  6. Influence of structural features on immunostimulating activity of glucans extracted from Agaricus blazei mushroom

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    Kozarski Maja S.

    2009-01-01

    Full Text Available High molecular weight b-D-glucans derived from Basidiomycetes cell walls are able to specifically activate cellular and humoral components of the host immune system. The aim of this paper was to examine immunomodulating activity of native, chemically and enzimatically modified glucans from Agaricus blazei mushroom and to determine which structural features are of primary importance for their stimulation referring to humane immune cells. The immunomodulating activities were tested in vitro, by stimulation of peripheral blood mononuclear cells (PBMCs and measuring of interferon-gamma (IFN-g production by enzyme linked immunosorbent assay (ELISA. Measurements of immunomodulatory capacity of Agaricus blazei native glucans showed their expressive immunostimulating effect on activated PBMCs and synthesis of IFN-g. The results obtained after the stimulation of cells with 1M H2SO4 and 1M NaOH, the treated glucans showed that primary structure is of more importance than the tertiary structure of the triple helix for their immunostimulating activity and synthesis of IFN-g. Glucans of lower molecular weight obtained after acid hydrolysis appeared as effective immunostimulators of PBMC's. The results obtained after the incubation of cells with 1,6 b-glucanase modified glucans suggest that b-(1,6 binding of glucose monomers probably has no importance for the production of imunostimulating effects, in vitro. This confirmed that b-(1,3 bonds are the primary determinants of immunomodulatory activities and stimulation of IFN-g synthesis.

  7. Agaricus blazei Murill as an efficient hepatoprotective and antioxidant agent against CCl4-induced liver injury in rats

    OpenAIRE

    Al-Dbass, Abeer M.; Al- Daihan, Sooad K.; Bhat, Ramesa Shafi

    2012-01-01

    Agaricus blazei Murill is one of the very popular edible medicinal mushrooms. The present study investigated the protective effect of this biologically active mushroom on the tissue peroxidative damage and abnormal antioxidant levels in carbon tetrachloride induced hepatotoxicity in male albino rats. Male albino rats of Sprague–Dawley strain weighting (120–150 g) were categorized into five groups. The first group served as the normal control, the second and the third groups were treated with ...

  8. Protoplast Preparation of Jisongrong Mushroom (Agaricus blazei)%姬松茸原生质体的制备

    Institute of Scientific and Technical Information of China (English)

    叶勋艳; 张云

    2005-01-01

    对姬松茸(Agaricus blazei Murrill)原生质体制备的最佳条件进行了研究,结果表明:采用液体发酵培养第 7 d的菌体,用2%溶壁酶在 30 ℃下酶解 5 h,原生质体产率达9.08×10 6个/mL.

  9. Effects of an Agaricus blazei Aqueous Extract Pretreatment on Paracetamol-Induced Brain and Liver Injury in Rats

    Directory of Open Access Journals (Sweden)

    Andréia A. Soares

    2013-01-01

    Full Text Available The action of an Agaricus blazei aqueous extract pretreatment on paracetamol injury in rats was examined not only in terms of the classical indicators (e.g., levels of hepatic enzymes in the plasma but also in terms of functional and metabolic parameters (e.g., gluconeogenesis. Considering solely the classical indicators for tissue damage, the results can be regarded as an indication that the A. blazei extract is able to provide a reasonable degree of protection against the paracetamol injury in both the hepatic and brain tissues. The A. blazei pretreatment largely prevented the increased levels of hepatic enzymes in the plasma (ASP, ALT, LDH, and ALP and practically normalized the TBARS levels in both liver and brain tissues. With respect to the functional and metabolic parameters of the liver, however, the extract provided little or no protection. This includes morphological signs of inflammation and the especially important functional parameter gluconeogenesis, which was impaired by paracetamol. Considering these results and the long list of extracts and substances that are said to have hepatoprotective effects, it would be useful to incorporate evaluations of functional parameters into the experimental protocols of studies aiming to attribute or refute effective hepatoprotective actions to natural products.

  10. Influence of Lentinus edodes and Agaricus blazei extracts on the prevention of oxidation and retention of tocopherols in soybean oil in an accelerated storage test.

    Science.gov (United States)

    da Silva, Ana Carolina; Jorge, Neuza

    2014-06-01

    This study aimed to evaluate the influence of the methanol extracts of mushrooms Lentinus edodes and Agaricus blazei on the retention of tocopherols in soybean oil, when subjected to an accelerated storage test. The following treatments were subjected to an accelerated storage test in an oven at 60 °C for 15 days: Control (soybean oil without antioxidants), TBHQ (soybean oil + 100 mg/kg of TBHQ), BHT (soybean oil + 100 mg/kg of BHT), L. edodes (soybean oil + 3,500 mg/kg of L. edodes extract) and A. blazei (soybean oil + 3,500 mg/kg of A. blazei extract). The samples were analyzed for tocopherols naturally present in soybean oil and mass gain. The results showed, the time required to reach a 0.5% increase in mass was 13 days for TBHQ and 15 days for A. blazei. The content of tocopherols for TBHQ was 457.50 mg/kg and the A. blazei, 477.20 mg/kg. PMID:24876658

  11. Serum hepatic biochemistry and electrophoretic protein profile of healthy and Ehrlich tumor-bearing mice treated with extracts of Agaricus blazei Murill

    Directory of Open Access Journals (Sweden)

    Durval Verçosa Junior

    2016-04-01

    Full Text Available Compounds isolated from Agaricus blazei Murill represent a group of promising natural immunomodulators for use in the treatment of neoplasms. We have evaluated the serum biochemical profile of healthy and Ehrlich tumor-bearing mice treated with different extracts of A. blazei. Total, supernatant, and polysaccharide extracts of A. blazei were obtained from suspensions (at acidic or neutral pH kept in a water bath at 60 °C or in an ultrasonic bath at 37 °C. After oral administering the extracts to mice for 21 days, blood samples were collected for determination of aspartate aminotransferase (AST, alanine aminotransferase (ALT, creatine kinase (CK, urea, total protein, albumin, globulins, and alpha-, beta- and gamma-globulin fractions. The presence of the tumor led to a significant increase in serum CK and AST activities and in the concentrations of total globulin and the gamma-globulin fraction, and to a decrease in the albumin and alpha2-globulin levels. The polysaccharide extracts of A. blazei reduced the serum AST and ALT activities, probably due to a hepatoprotective effect. In addition, polysaccharide and supernatant extracts inhibited the tumor-induced increase in gamma-globulin levels. Thus, the supernatant and polysaccharide fractions of the extract of A. blazei have potential for use in complementary antineoplastic treatments.

  12. Avaliação in vitro do crescimento micelial de cinco linhagens de Agaricus blazei em duas temperaturas / In vitro evaluation of the mycelial growth of five Agaricus blazei strains in two temperatures

    Directory of Open Access Journals (Sweden)

    Diego Cunha Zied

    2011-04-01

    Full Text Available O objetivo do trabalho foi avaliar o crescimento micelial in vitro de cinco linhagens de Agaricus blazei (ABL-05/53, ABL-04/49, ABL-03/44, ABL-99/30 e ABL-02/51, quando submetidas às temperaturas de 20 e 25ºC. Em câmara de fluxo laminar, discos das linhagens foram inoculadas no centro de placas de Petri contendo o meio CA (composto-ágar e incubadas em estufa BOD. Após 48 horas, iniciaram-se as medidas do crescimento micelial, com auxílio de uma régua graduada em milímetros, através de quatro medições equidistantes entre si, até o momento em que, em um dos tratamentos, a colônia fúngica atingisse a proximidade das bordas da placa de Petri. O delineamento experimental foi inteiramente casualizado, em esquema fatorial 5 x 2. Cada tratamento constou de sete repetições, correspondente a uma placa de Petri, totalizando setenta unidades experimentais. Verificou-se que o crescimento de A. blazei é influenciado pela temperatura de incubação, sendo que a temperatura de 25°C foi mais favorável para o crescimento micelial de todas as linhagens de A. blazei avaliadas, com destaque para as linhagens ABL-04/49 e ABL-03/44 que obtiveram as maiores médias de crescimento micelial nesta condição de temperatura ao final do ciclo de cultivo.AbstractThe objective of the work was to evaluate the in vitro mycelial growth of five A. blazei strains (ABL-05/53, ABL-04/49, ABL-03/44, ABL-99/30 and ABL-02/51 when submitted to the temperatures of 20 and 25 ºC. In a laminar flow chamber, discs of the strains were inoculated in the middle of Petri’s plates containing CA (compost-agar medium and incubated in BOD. After 48 hours, measurements of the mycelial growth began, with the help of a ruler with scale in millimeters, by means of four equidistant measurements, until the moment when the fungal colony reached near the edges of the Petri’s plate in one of the treatments. The experimental design was totally randomized, in 5 x 2 factorial design

  13. Avaliação da coloração de basidiocarpos desidratados de Agaricus blazei segundo escala de Munsell Evaluation of the dehydrated fruiting bodies of the mushroom Agaricus blazei according to Munsell colour scale

    Directory of Open Access Journals (Sweden)

    Marisa Aparecida da Fonseca Delú

    2006-02-01

    Full Text Available Com este trabalho objetivou-se avaliar a coloração final de basidiocarpos desidratados de Agaricus blazei, após serem submetidos a tratamento com ácidos ascórbico e cítrico. Para tanto, utilizaram-se cogumelos A. blazei, cultivados em substrato apropriado e mantidos em estufa. Os cogumelos foram colhidos com o píleo totalmente fechado e após a limpeza em água corrente, os basidiocarpos foram seccionados ao meio no sentido longitudinal, sendo uma metade submetida ao tratamento experimental e a outra metade representando o tratamento controle. Os tratamentos experimentais consistiram da imersão das metades dos basidiocarpos em solução de ácido ascórbico comercial nas concentrações de 0, 10, 20 e 30 mg/L durante 0, 2, 5, 10, 15 ou 20 minutos. Para o ácido cítrico foram testadas as mesmas concentrações anteriores, porém nos tempos de imersão de 0, 10 e 15 minutos. Todos os tratamentos consistiram de 3 repetições cada. Não se observou relação entre os tratamentos utilizados com a coloração final dos basidiocarpos, embora a utilização de substâncias ácidas tenha possibilitado a obtenção de basidiocarpos desidratados mais próximos daquela coloração considerada ideal para comercialização.The main aim of this work was to evaluate the final colour of the fructification bodies of the mushroom Agaricus blazei submitted to ascorbic and citric acid treatment. The mushroom Agaricus blazei was cultivated in an appropriated substrate and storage in incubators. The mushroom was harvested containing closed pileus and after cleaning with running tap water, the fruiting bodies were sliced and half of each were submitted to acid treatment and half was the control (water immersion. The experimental treatment consisted of immersing half of each basiodiocarp in different concentrations of the commercial solution of ascorbic acid (0, 10, 20 and 30 mg/ml during 0, 2, 5, 10, 15 and 20 minutes. The other parts of fruiting bodies were

  14. The Medicinal Mushroom Agaricus blazei Murrill: Review of Literature and Pharmaco-Toxicological Problems

    Directory of Open Access Journals (Sweden)

    F. Firenzuoli

    2008-01-01

    Full Text Available Agaricus blazei Murrill (ABM popularly known as ‘Cogumelo do Sol’ in Brazil, or ‘Himematsutake’ in Japan, is a mushroom native to Brazil, and widely cultivated in Japan for its medicinal uses, so it is now considered as one of the most important edible and culinary-medicinal biotechnological species. It was traditionally used to treat many common diseases like atherosclerosis, hepatitis, hyperlipidemia, diabetes, dermatitis and cancer. In vitro and in vivo ABM has shown immunomodulatory and antimutagenic properties, although the biological pathways and chemical substances involved in its pharmacological activities are still not clear. The polysaccharides phytocomplex is thought to be responsible for its immunostimulant and antitumor properties, probably through an opsonizing biochemical pathway. Clinical studies are positive confirmations, but we are still at the beginning, and there are perplexing concerns especially relative to the content of agaritine. Argantine is a well-known carcinogenic and toxic substance in animals, that must be completely and fully evaluated.

  15. Phase I Clinical Study of the Dietary Supplement, Agaricus blazei Murill, in Cancer Patients in Remission

    Directory of Open Access Journals (Sweden)

    Satoshi Ohno

    2011-01-01

    Full Text Available Although many cancer patients use complementary and alternative medicine, including Agaricus blazei Murill (ABM, safety is not yet well understood. Cancer survivors took 1.8, 3.6, or 5.4 g ABM granulated powder (Kyowa Wellness Co., Ltd., Tokyo, Japan per day orally for 6 months. Adverse events were defined by subjective/objective symptoms and laboratory data according to the National Cancer Institute Common Terminology Criteria for Adverse Events version 3.0 (NCI-CTCAE v3.0. Seventy-eight patients were assessed for safety of ABM (30/24/24 subjects at 1/2/3 packs per day, resp.. Adverse events were observed in 9 patients (12%. Most were digestive in nature such as nausea and diarrhea, and one patient developed a liver dysfunction-related food allergy, drug lymphocyte product. However, none of these adverse events occurred in a dose-dependent manner. This study shows that ABM does not cause problems in most patients within laboratory parameters at the dosages tested over 6 months. This trial supports previous evidence that the ABM product is generally safe, excluding possible allergic reaction.

  16. Influência de Agaricus blazei Murrill sobre o tumor sólido de Ehrlich e linfonodos poplíteos de camundongos Influence of Agaricus blazei Murrill in solid Ehrlich tumor and popliteal lymph nodes in mice

    Directory of Open Access Journals (Sweden)

    D. Verçosa Junior

    2007-02-01

    Full Text Available Estudou-se a influência do tratamento diário com filtrado aquoso de Agaricus blazei Murrill (ABM (25mg/ml, via oral, por 17 e 57 dias, em camundongos inoculados com tumor sólido de Ehrlich (TSE por meio da curva de crescimento tumoral, do peso relativo do tumor, da contagem de regiões organizadoras de nucléolos (AgNORs e dos padrões histológicos das massas tumorais e linfonodos poplíteos. Os animais que ingeriram o extrato aquoso do ABM por 57 dias apresentaram menor (P0,05 entre os animais tratados e não tratados.The influence of daily administration of Agaricus blazei Murrill (ABM aqueous solution (25mg/ml during 17 and 57 days in mice bearing solid Ehrlich tumor was studied. Tumoral growth, tumoral and spleenic relative weights, nucleoly organization regions AgNORs values and tumor and popliteal lymph nodes histopathology were daily evaluated. The animals that received ABM during 57 days showed lower values (P0.05 between treated and non-treated animals. No difference in microscopic evaluation of the tumors in treated and non-treated animals was seen and metastasis in popliteal nodes of the tumor occurred in all the animals.

  17. Extratos de Lentinula edodes e Agaricus blazei sobre Bipolaris sorokiniana e Puccinia recondita f. sp. tritici, in vitro Extracts of Lentinula edodes and Agaricus blazei on Bipolaris sorokiniana and Puccinia recondita f. sp. tritici., in vitro

    Directory of Open Access Journals (Sweden)

    Ana Cristina Grade Fiori-Tutida

    2007-09-01

    Full Text Available Com o objetivo de buscar medidas alternativas para o controle de Bipolaris sorokiniana e Puccinia recondita f. sp. tritici testou-se o efeito fungitóxico in vitro dos cogumelos Lentinula edodes e Agaricus blazei sobre esses fungos. Os extratos brutos aquosos de ambos os cogumelos não tiveram efeito significativo tanto no crescimento micelial quanto na germinação de esporos de B. sorokiniana. Por outro lado, os extratos dos cogumelos inibiram a germinação de esporos de P. recondita f. sp. tritici, com destaque para o isolado LE 96/17 de L. edodes que apresentou inibição da ordem de 52,4%.With the purpose of finding alternative ways to control Bipolaris sorokiniana and Puccinia recondita f. sp. triciti, the fungitoxic effect in vitro of Lentinula edodes and Agaricus blazei mushrooms was tested on these fungi. The aqueous crude extract of both mushrooms did not have significant effect on mycelial growth and spore germination of B. sorokiniana . On the other hand, the mushroom extracts inhibited the germination of P. recondita f. sp. tritici uredospores, particularly on the L. edodes (96/17, which exhibited the largest inhibition of spore germination (52,38%.

  18. The Mushroom Agaricus blazei Murill Elicits Medicinal Effects on Tumor, Infection, Allergy, and Inflammation through Its Modulation of Innate Immunity and Amelioration of Th1/Th2 Imbalance and Inflammation

    OpenAIRE

    Geir Hetland; Egil Johnson; Torstein Lyberg; Gunnar Kvalheim

    2011-01-01

    The medicinal mushroom Agaricus blazei Murill from the Brazilian rain forest has been used in traditional medicine and as health food for the prevention of a range of diseases, including infection, allergy, and cancer. Other scientists and we have examined whether there is scientific evidence behind such postulations. Agaricus blazei M is rich in the immunomodulating polysaccharides, β -glucans, and has been shown to have antitumor, anti-infection, and antiallergic/-asthmatic properties in mo...

  19. 19 July 2013 - Chairman of the Policy Committee, European Cancer Organisation, President, European Association for Cancer Research E. Celis visiting the ATLAS experimental cavern with ATLAS Collaboration Deputy Spokesperson, B. Heinemann and signing the Guest Book with Director for Accelerators and Technology S. Myers. Life Sciences Adviser M. Dosanjh present.

    CERN Multimedia

    Anna Pantelia

    2013-01-01

    19 July 2013 - Chairman of the Policy Committee, European Cancer Organisation, President, European Association for Cancer Research E. Celis visiting the ATLAS experimental cavern with ATLAS Collaboration Deputy Spokesperson, B. Heinemann and signing the Guest Book with Director for Accelerators and Technology S. Myers. Life Sciences Adviser M. Dosanjh present.

  20. 23 July - Italian Director-General for Prevention G. Ruocco and Director-General for European and International Relations Ministry of Health D. Roderigo visiting the ATLAS experimental cavern with ATLAS Deputy Spokesperson B. Heinemann. Life Sciences Section M. Cirilli and Life Sciences Adviser M. Dosanjh present.

    CERN Multimedia

    Anna Pantelia

    2013-01-01

    23 July - Italian Director-General for Prevention G. Ruocco and Director-General for European and International Relations Ministry of Health D. Roderigo visiting the ATLAS experimental cavern with ATLAS Deputy Spokesperson B. Heinemann. Life Sciences Section M. Cirilli and Life Sciences Adviser M. Dosanjh present.

  1. Antimutagenic action of Lentinula edodes and Agaricus blazei on Aspergillus nidulans conidia Ação antimutagênica de Lentinula edodes e Agaricus blazei em conídios de Aspergillus nidulans

    Directory of Open Access Journals (Sweden)

    Edneia A. Souza-Paccola

    2004-12-01

    Full Text Available The antimutagenic effect of the mushrooms Lentinula edodes and Agaricus blazei was studied on conidia of Aspergillus nidulans when exposed to short wave ultraviolet light. Two strains of A. nidulans were used. For the preparation of the extracts, the fresh mushrooms were left in aqueous infusion for 12 hours and heated in a water bath for 15 min at 100ºC, and then the material was filtered. The dehydrated mushrooms were left in aqueous infusion for 12 hours and to filtrated. Both filtrates were used as extracts. A. nidulans conidia were incubated for three hours in water and in mushroom extracts and only after were exposed to UV light (pre-treatment. A. nidulans conidia were suspended in water and in mushroom extracts and immediately submitted to UV light (post-treatment. Conidial suspension in water and in mushroom extracts but without exposure to the mutagenic agent were used as controls. After mutagenic treatment, it was observed an increase in the survival rate of the A. nidulans and a decrease in the percentage of morphologic mutants on conidia treated with mushroom extracts. Our results demonstrated the radioprotective and antimutagenic effect of L. edodes and A. blazei mushrooms on eukaryotic cells when exposed to UV radiation.O efeito antimutagênico dos cogumelos Lentinula edodes e Agaricus blazei foram estudados sobre conídios de Aspergillus nidulans quando expostos à luz ultravioleta de comprimento de onda curto. Duas linhagens de A. nidulans foram usadas. Para o preparo dos extratos, os cogumelos frescos permaneceram em infusão aquosa por 12 horas e em seguida foram aquecidos em banho-maria por 15 min à 100ºC e a seguir o material foi filtrado. Os cogumelos desidratados foram deixados em infusão aquosa por 12 horas e a seguir filtrados. Ambos os filtrados foram usados como extratos. Os conídios de A. nidulans foram incubados por três horas em água e em extrato de cogumelo e somente após foram expostos a luz ultravioleta

  2. Common desease and insect in Agaricus blazei and their control%巴西蘑菇常见病虫害及防治

    Institute of Scientific and Technical Information of China (English)

    林衍铨; 黄建成; 胡润芳

    2000-01-01

    @@ 巴西蘑菇(Agaricus blazei Murrill,日本商品名称姬松茸)在栽培过程中,往往会出现各种病、虫害,轻者降低品质,造成减产,重者甚至绝收.现将巴西蘑菇常见的病虫害及其防治措施介绍如下.

  3. 巴西蘑菇Agaricus blazei Murill水溶性多糖部分理化性质的研究%Some Properties of Water - soluble Polysaccharides from Agaricus blazei Murill Fruit Body

    Institute of Scientific and Technical Information of China (English)

    林跃鑫; 叶竹秋; 黄谚谚; 谢华玲

    2002-01-01

    本文以物理及化学方法研究了巴西蘑菇Agaricus blazei Murill水溶性多糖AB-Ⅰ、AB-Ⅱ-b和AB-Ⅲ-b的理化性质,结果表明,3种经纯化的多糖都不含糖醛酸而含蛋白质,蛋白质含量分别为AB-Ⅰ:20.1%、AB-Ⅱ-b:16.3%、AB-Ⅲ-b:25.6%.它们的比旋度分别为AB-Ⅰ:+44.33°、AB-Ⅱ-b;+3.70°、AB-Ⅲ-b:+5.04°.AB-Ⅰ、AB-Ⅱ-b的1.0%水溶液均具有良好的增稠性,其粘度具有很好的抗盐浓度、耐热和对广泛pH的稳定性能.

  4. 76 FR 17988 - Proposed Collection; Comment Request for Form SS-4 and SS-4PR

    Science.gov (United States)

    2011-03-31

    ... Internal Revenue Service Proposed Collection; Comment Request for Form SS-4 and SS-4PR AGENCY: Internal...(c)(2)(A)). Currently, the IRS is soliciting comments concerning Form SS-4, Application for Employer Identification Number, and Form SS- 4PR, Solicitud de Numero de Indentification Patronal (EIN). DATES:...

  5. Aqueous Extract of Agaricus blazei Murrill Prevents Age-Related Changes in the Myenteric Plexus of the Jejunum in Rats

    Directory of Open Access Journals (Sweden)

    Ana Paula de Santi-Rampazzo

    2015-01-01

    Full Text Available This study evaluated the effects of the supplementation with aqueous extract of Agaricus blazei Murrill (ABM on biometric and blood parameters and quantitative morphology of the myenteric plexus and jejunal wall in aging Wistar rats. The animals were euthanized at 7 (C7, 12 (C12 and CA12, and 23 months of age (C23 and CA23. The CA12 and CA23 groups received a daily dose of ABM extract (26 mg/animal via gavage, beginning at 7 months of age. A reduction in food intake was observed with aging, with increases in the Lee index, retroperitoneal fat, intestinal length, and levels of total cholesterol and total proteins. Aging led to a reduction of the total wall thickness, mucosa tunic, villus height, crypt depth, and number of goblet cells. In the myenteric plexus, aging quantitatively decreased the population of HuC/D+ neuronal and S100+ glial cells, with maintenance of the nNOS+ nitrergic subpopulation and increase in the cell body area of these populations. Supplementation with the ABM extract preserved the myenteric plexus in old animals, in which no differences were detected in the density and cell body profile of neurons and glial cells in the CA12 and CA23 groups, compared with C7 group. The supplementation with the aqueous extract of ABM efficiently maintained myenteric plexus homeostasis, which positively influenced the physiology and prevented the death of the neurons and glial cells.

  6. 姬松茸液体培养基的筛选%The Selection on Culture Medium of Agaricus blazei Murrill

    Institute of Scientific and Technical Information of China (English)

    周选围

    2001-01-01

    Agaricus blazei Murrill is one of the edible and m edicinal fungi.In this paper,through the selection and study of the improted Agaricus blazei Murrill in submerged culture medium,the experimental r esults show that the most suitable composition of the Agaricus blazei Murrill submerged culture medium is as follow:3.0% corn powder,0.3% yeast conc e ntration,2.0% sugar,0.05%(NH4)2SO4,0.1%MgSO4,0.1%KH2PO4.In thi s liquid medium,the mycelium grows vigorous,it gets to 539mg/100mL(DW)for 8 d ays.%对引进的姬松茸菌种进行了液体培养基的筛选研究,认为姬松茸液体培养最适合的培养基配方为:玉米粉3.0%,酵母提取物0.3%,蔗糖2.0%,硫酸铵0 .05%,硫酸镁0.1%,磷酸二氢钾0.1%。在这种培养基中,姬松茸生长旺盛,产生的菌丝球数量多,颜色白,大小均一,8d后生物量达53.9%mg/L(DW)。

  7. 姬松茸颗粒剂扶正作用的实验研究%Researching on Experimentation in strengthening body resistance of Agaricus blazei Muriu

    Institute of Scientific and Technical Information of China (English)

    王伟明; 张云凌

    2004-01-01

    姬松茸(Agaricus blazei Muriu)又称巴西菇,是一种食、药两用真菌,姬松茸富含多种丰富的营养成分。姬松茸颗粒剂系采用新工艺从姬松茸中提取的主要成分为粗多糖的一种制剂,我们对其抗肿瘤和对免疫功能的影响等方面进行了实验研究,现报道如下。

  8. 工厂化栽培姬松茸的关键技术%Factory Cultivation Key Techniques of Agaricus blazei

    Institute of Scientific and Technical Information of China (English)

    曾先富; 熊维全; 李昕竺; 刘洪

    2015-01-01

    姬松茸(Agaricus blazei)是一种珍稀食(药)用菌.对姬松茸工厂化栽培技术中菌种选择与制作、培养料配方与发酵、铺料与播种、菌丝体培养、覆土、出菇管理、采收与转潮管理、病虫害绿色防控等关键技术点进行了介绍.

  9. Studies on Odontotermes formosanus Food Choice between Agaricus blazei and Its Substrate%黑翅土白蚁对姬松茸及其培养料的取食性研究

    Institute of Scientific and Technical Information of China (English)

    苏德伟; 林辉; 罗海凌; 林春梅; 林占熺

    2012-01-01

    In order to evaluate the impact of Odontotermes formosanus to Agaricus blazei Murrill cultivation, studies were carried out on food selectiveness of Odontotermes formosanus by observing 5 different materials ( fruitbody of Agaricus blazei Murrill, bamboo powder, Juncao, Juncao substrate of Agaricus blazei Murrill, bamboo substrate of Agaricus blazei Murrill) foraging behaviors. The results showed that consumption rate of Juncao and bamboo powders were very significantly higher than that of other 3 materials. And the consumption rate of Agaricus blazei Murrill was 0. The mud sheet area and mud sheet coverage rate of Juncao and bamboo powders were very significantly higher than that of the other 3 materials. These all indicated that the volatile substances distributed from mycelium and fruitbody of Agaricus blazei Murrill could inhibit termites' feeding. And the harms of termites in Agaricus blazei Murrill were mainly from cultivation materials.%通过观察黑翅土白蚁工蚁的觅食行为,进行了黑翅土白蚁对5种材料(姬松茸、木屑、菌草草粉、菌草姬松茸培养料、木屑姬松茸培养料)的取食选择性研究.结果表明,木屑和菌草草粉的被食率极显著高于其他3种材料,而姬松茸被食率为0;木屑与菌草草粉的泥被面积和泥被覆盖率极显著高于其他3种材料.这都表明姬松茸菌丝及其子实体散发出来的挥发性物质可以抑制黑翅土白蚁取食,黑翅土白蚁为害姬松茸主要是对其栽培料的破坏.

  10. Primary mechanism of apoptosis induction in a leukemia cell line by fraction FA-2-b-ß prepared from the mushroom Agaricus blazei Murill

    Directory of Open Access Journals (Sweden)

    L. Gao

    2007-11-01

    Full Text Available Agaricus blazei Murill is a native Brazilian mushroom which functions primarily as an anticancer substance in transplanted mouse tumors. However, the mechanism underlying this function of A. blazei Murill remains obscure. The present study was carried out to investigate the effect of fraction FA-2-b-ß, an RNA-protein complex isolated from A. blazei Murill, on human leukemia HL-60 cells in vitro. Typical apoptotic characteristics were determined by morphological methods using DNA agarose gel electrophoresis and flow cytometry. The growth suppressive effect of fraction FA-2-b-ß on HL-60 cells in vitro occurred in a dose- (5-80 µg/mL and time-dependent (24-96 h manner. The proliferation of HL-60 cells (1 x 10(5 cells/mL treated with 40 µg/mL of fraction FA-2-b-ß for 24-96 h and with 5-80 µg/mL for 96 h resulted in inhibitory rates ranging from 8 to 54.5%, and from 4.9 to 86.3%, respectively. Both telomerase activity determined by TRAP-ELISA and mRNA expression of the caspase-3 gene detected by RT-PCR were increased in HL-60 cells during fraction FA-2-b-ß treatment. The rate of apoptosis correlated negatively with the decrease of telomerase activity (r = 0.926, P < 0.05, but correlated positively with caspase-3 mRNA expression (r = 0.926, P < 0.05. These data show that fraction FA-2-b-ß can induce HL-60 cell apoptosis and that the combined effect of down-regulation of telomerase activity and up-regulation of mRNA expression of the caspase-3 gene could be the primary mechanism of induction of apoptosis. These findings provide strong evidence that fraction FA-2-b-ß could be of interest for the clinical treatment of acute leukemia.

  11. 28 November 2013 - N. N. Kudryavtsev, Russian Rector of the Moscow Institute of Physics and Technology signing an Agreement and the Guest Book with CERN Director-General R. Heuer; visiting the ATLAS cavern with ATLAS Deputy Spokesperson B. Heinemann and visiting the LHC tunnel at Point 1 with AGH University of Science and Technology A. Erokhin. M. Savino, Physics Department, Joint Institute for Nuclear Research also present.

    CERN Multimedia

    Jean-Claude Gadmer

    2013-01-01

    28 November 2013 - N. N. Kudryavtsev, Russian Rector of the Moscow Institute of Physics and Technology signing an Agreement and the Guest Book with CERN Director-General R. Heuer; visiting the ATLAS cavern with ATLAS Deputy Spokesperson B. Heinemann and visiting the LHC tunnel at Point 1 with AGH University of Science and Technology A. Erokhin. M. Savino, Physics Department, Joint Institute for Nuclear Research also present.

  12. Use of Agaricus blazei and Lentinula edodes extracts for post-harvest control of gray mold in ‘Itália’ grape Extracto de Agaricus blazei y Lentinula edodes en el control de podredumbre gris en uva ‘Itália’ Extrato de Agaricus blazei e Lentinula edodes no controle pós-colheita de mofo cinzento em uva ‘Itália’

    Directory of Open Access Journals (Sweden)

    ELISANGELA CLARETE CAMILI

    2010-04-01

    Full Text Available

    Na proteção de uva ‘Itália’ pós-colheita contra Botrytis cinerea avaliou-se in vivo o efeito direto e indireto dos extratos aquosos dos cogumelos Agaricus blazei e Lentinula edodes através do tratamento dos cachos antes ou após a inoculação com o patógeno. Para tanto se realizaram dois ensaios: 1 cachos de uva foram inoculados e, após 4 h, aspergidos com diferentes concentrações dos extratos dos basidiocarpos (0,0; 2,5; 5,0; 10,0; 20,0 ou 40,0%; 2 cachos foram, ou não, aspergidos com extrato de A. blazei (5,0% 24, 48, 72 ou 96 h antes da inoculação do fungo. Para inoculação, em cada cacho foram feridas 10 bagas, fazendo-se um furo por baga de ±2 mm de profundidade, procedendo-se em seguida, a aspersão da suspensão de conídios (±105 conídios mL-1. Após os tratamentos, os cachos foram mantidos a 25±1 ºC/80-90% UR e avaliados diariamente quanto à incidência e severidade da podridão. O efeito in vitro dos extratos de A. blazei e L. edodes no controle do patógeno foi avaliado com o intuito de verificar se tais agentes exercem efeito direto sobre o crescimento micelial e a germinação de conídios de B. cinerea. Os resultados mostraram que os extratos de A. blazei e L. edodes não controlaram a podridão causada por B. cinerea em uva ‘Itália’, quando aplicados antes ou após a inoculação do fungo. Nos ensaios in vitro, ambos os extratos dos basidiocarpos estimularam a germinação dos conídios de B. cinerea nas concentrações testadas; quanto ao crescimento micelial, L. edodes retardou, enquanto A. blazei estimulou.

    For protecting ‘Itália’ against Botrytis cinerea in the post harvest it was evaluated in vivo direct and indirect effect of water based extracts of Agaricus blazei and Lentinula edodes

  13. The Mushroom Agaricus blazei Murill Elicits Medicinal Effects on Tumor, Infection, Allergy, and Inflammation through Its Modulation of Innate Immunity and Amelioration of Th1/Th2 Imbalance and Inflammation

    Directory of Open Access Journals (Sweden)

    Geir Hetland

    2011-01-01

    Full Text Available The medicinal mushroom Agaricus blazei Murill from the Brazilian rain forest has been used in traditional medicine and as health food for the prevention of a range of diseases, including infection, allergy, and cancer. Other scientists and we have examined whether there is scientific evidence behind such postulations. Agaricus blazei M is rich in the immunomodulating polysaccharides, β-glucans, and has been shown to have antitumor, anti-infection, and antiallergic/-asthmatic properties in mouse models, in addition to anti-inflammatory effects in inflammatory bowel disease patients. These effects are mediated through the mushroom's stimulation of innate immune cells, such as monocytes, NK cells, and dendritic cells, and the amelioration of a skewed Th1/Th2 balance and inflammation.

  14. 姬松茸母种培养基筛选研究初报%A Preliminary Study on the Selection of Mother Culture Media of Agaricus blazei Mürrill

    Institute of Scientific and Technical Information of China (English)

    陈文强; 周选围; 邓百万

    2002-01-01

    对姬松茸(Agaricus blazei Mtirrill)进行了母种培养基筛选试验.结果表明,姬松茸母种的最适培养基为:蔗糖25g,玉米淀粉30g,磷酸二氢钾3g,硫酸铵3g,维生素B1 10mg,琼脂20g.

  15. Research on the technology of composting sugarcane leaf in a fermentation tunnel for Agaricus blazei murill. cultivation%姬松茸蔗叶培养料隧道式发酵技术研究

    Institute of Scientific and Technical Information of China (English)

    谢毅栋; 覃喜强; 黄华

    2015-01-01

    通过对比糖料蔗叶和稻草为主原料,以隧道发酵和常规堆沤两种发酵方式制备栽培姬松茸的培养料研究,形成培养料制备过程中温度变化曲线。结果表明,以蔗叶为主原料栽培姬松茸完全可行,隧道发酵比常规堆沤时间大幅缩短,培养料质量稳定,能大幅提高姬松茸产量。%Through studying the research results for two different methods (fermentation tunnel and regular fermentation) of cultivating composting sugarcane leaf and straw for Agaricus blazei murill. plantation, as well as the temperature change records during the production of the compost, the experiment proves that it is doable to utilize sugarcane based compost to cultivate Agaricus blazei murill. Fermentation in a tunnel is dramatically faster than the regular fermentation method. The quality of the compost is stable and can increase the production of the Agaricus blazei murill.

  16. 47 CFR 97.311 - SS emission types.

    Science.gov (United States)

    2010-10-01

    ... 47 Telecommunication 5 2010-10-01 2010-10-01 false SS emission types. 97.311 Section 97.311... SERVICE Technical Standards § 97.311 SS emission types. (a) SS emission transmissions by an amateur... station in another country that permits such communications. SS emission transmissions must not be...

  17. Huumori toimintaterapeutin työssä

    OpenAIRE

    Liinamo, Liisa

    2006-01-01

    Koko teksti, Tiivistelmä, Abstract Tutkimuksen tarkoituksena oli kuvata huumoria toimintaterapeutin työssä. Tutkimuksen aineistona oli neljän toimintaterapeutin haastattelut, joissa pohdittiin, mitä huumori oli, ilmenikö sitä toimintaterapiassa, miten sitä ilmeni ja mitä merkitystä huumorilla oli toimintaterapeutin työssään. Haastatteluaineistoon perehdyttiin käyttäen aineistolähtöistä sisällön analyysiä. Teoriaosuudessa huumoria tarkasteltiin eri tieteenalojen- ja toimintaterapiakirjallis...

  18. SS 433: Stationary lines and primary eclipses

    CERN Document Server

    Bowler, M G

    2015-01-01

    Some stationary lines in the emission spectra of SS 433 are eclipsed, but most are not. Lines attributed to a circumbinary disk are not eclipsed, but double in relative intensity during primary eclipse. A C II doublet is eclipsed and Doppler shifts over two periods yield an orbital speed of 176 +/- 13 km/s.

  19. Asiantuntijapalvelun tuotteistaminen : yhteistyössä vastuullisuutta edistämässä

    OpenAIRE

    Asteljoki, Kaj

    2010-01-01

    Työssä on liitetty yhteen vastuullinen liiketoiminta ja strateginen johtaminen kestävän kehityksen näkökulmasta, tavoitteena asiakasyrityksen pitkän aikavälin menestyminen. Vastuullinen liiketoiminta on organisaatiolle strateginen valinta, jonka sudenkuoppana on käytäntöön vieminen. Tämä sama ongelma on yleinen strategisessa johtamisessa. Tässä työssä kehitetään vastuullisen liiketoimintastrategian laatimiseen ja käytäntöön viemiseen soveltuvia yhteistoiminnallisia, osallistavia menettelyjä. ...

  20. SS433: The microquasar link with ulxs?

    Directory of Open Access Journals (Sweden)

    P. A. Charles

    2004-01-01

    Full Text Available SS433 es el microcuasar prototipo de la Galaxia y posiblemente incluso an alogo a las fuentes ULX si se tiene en cuenta la energ a cin etica de los \\jets". Sin embargo y a pesar de 20 a~nos de estudio, nuestras restricciones sobre la naturaleza del sistema binario son extremadamente grandes debido a la di cultad de localizar rasgos espectrales capaces de revelar la naturaleza y el movimiento del donador de la masa. Los espectros azules de alta resoluci on recientemente obtenidos cuando el disco (en precesi on se encuentra de per l, sugieren que la binaria se halla cerca de la fase de envolvente com un, lo cual hace extremadamente dif cil obtener las restricciones cin eticas. Sin embargo, encontramos evidencia de un donador masivo como era de esperar para la muy alta tasa de transferencia de masa inferida y comparamos las propiedades de SS433 con las de Cyg X3.

  1. Toivo psykiatrisessa hoitotyössä

    OpenAIRE

    Jokela, Ville; Moberg, Hanna

    2016-01-01

    Opinnäytetyön tarkoituksena oli selvittää hoitajien näkökulmasta potilaan toivon ilmenemistä ja tukemista psykiatrisessa hoitotyössä. Opinnäytetyön aihe tuli Pirkanmaan sairaanhoitopiiriltä. Opinnäytetyön tehtävinä oli selvittää, miten toivo ilmenee psykiatrisessa hoitotyössä, mikä on hoitajan merkitys potilaan toivon kannalta, millä käytännön hoitotyön keinoilla potilaan toivoa voidaan tukea, mikä merkitys sosiaalisilla suhteilla on potilaan toivolle sekä miten toivottomuus ilmenee psykiatri...

  2. Oral Treatment with Extract of Agaricus blazei Murill Enhanced Th1 Response through Intestinal Epithelial Cells and Suppressed OVA-Sensitized Allergy in Mice

    Directory of Open Access Journals (Sweden)

    Go Bouike

    2011-01-01

    Full Text Available To clarify the mechanism of the antiallergic activity of Agaricus blazei Murill extract (ABME, the present paper used an in vivo allergy model and an in vitro intestinal gut model. During OVA sensitization, the serum IgE levels decreased significantly in ABME group. Interleukin (IL-4 and -5 produced from OVA-restimulated splenocytes was significantly decreased, and anti-CD3ε/CD28 antibody treatment also reduced IL-10, -4, and -5 production and increased IFN-γ production in ABME group. These results suggest that oral administration of ABME improves Th1/Th2 balance. Moreover, a coculture system constructed of Caco-2 cells and splenocytes from OT-II mice or RAW 264.7 cells indicated that the significant increases in IFN-γ production by ABME treatment. Therefore, it was concluded that the antiallergic activity of ABME was due to the activation of macrophages by epithelial cells and the promotion of the differentiation of naïve T cells into Th1 cells in the immune.

  3. Blood parameters of sheep with high infection of Haemonchus contortus and treated with “mushroom of the sun” (Agaricus blazei

    Directory of Open Access Journals (Sweden)

    Gabriela Almeida Bastos

    2016-04-01

    Full Text Available Blood and parasitological parameters of sheep experimentally infected with Haemonchus contortus and treated with “mushroom of the sun” (Agaricus blazei were assessed. Lambs infected with the nematode were divided into three groups: treated with basidiocarp powder from the fungus, wormed with trichlorfon, and a control group that did not receive anthelmintic. Split-plot design analysis was performed where the treatments were defined as plots and four periods of collection were defined as subplots. Interaction between the treatments and the collection periods for the fecal egg counts per gram of feces (FEC was observed. Treatment with “mushroom of the sun” showed anthelmintic efficacies ranging from 28.6 to 54.2%. Similar performances to weight gain were observed among the groups. A significant interaction between treatments and evaluation periods was observed for erythrocyte counts, hematocrit value, and serum concentrations of albumin and urea. The values of the mean corpuscular volume, erythrocyte distribution width, and leukocyte, neutrophil, lymphocyte, monocyte, and eosinophil counts, as well as the mean platelet volume, varied among the collection days. The administration of “mushroom of the sun” significantly reduced the FEC, did not influence on the erythrocyte count and hematocrit value, which remained within normal limits. However it increased the serum concentrations of albumin.

  4. Optimization of Inter-Simple Sequence Repeats(ISSR) Reaction System for Agaricus blazei%姬松茸ISSR特异扩增体系的研究

    Institute of Scientific and Technical Information of China (English)

    林新坚; 江秀红; 蔡海松; 郑永标; 林陈强

    2007-01-01

    为了建立稳定的姬松茸(Agaricus blazei Murill)简单序列重复区间(Inter-Simple Sequence Repeats,ISSR)分子标记技术体系,笔者通过单因子试验分别研究了模板DNA、Mg2+浓度、dNTP、引物浓度和Taq酶用量对姬松茸ISSR-PCR扩增的影响,确定了姬松茸ISSR分析的最佳PCR条件为:25μL反应体系中,模板DNA 20 ng,引物0.75μmol/L,dNTP 200μmol/L,Mg2+2.0 mmol/L,Taq DNA polymerase 1.5 U.并应用该优化体系筛选到6个适合姬松茸ISSR-PCR扩增的引物,为利用ISSR标记技术研究姬松茸的种质资源提供了参考.

  5. SS wireless modem for an automatic vending machine; Jido hanbaikiyo SS musen modemu

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2000-01-10

    Low-cost network communication systems are required when introducing an information collecting system and an electronic money system for automatic vending machines. Development was made recently on a spread spectrum (SS) wireless modem capable of structuring a network incurring no running cost for wiring works and communications. The modem is intended of being used at locations where automatic vending machines are installed at relatively high concentration, such as railway stations, buildings and car parking areas. Its major features are as follows: (1) packet transfer is performed while data are relayed at high speed between arbitrary SS wireless modems; (2) high reliability communication is possible because an optimal relaying route is selected even at line impediments or failure of the SS wireless modems; and (3) the modem can be applied even to large-scale systems. (translated by NEDO)

  6. Potilasturvallisuus perioperatiivisessa hoitotyössä -systemaattinen kirjallisuuskatsaus

    OpenAIRE

    Hartikainen, Saara

    2012-01-01

    ssä opinnäytetyössä tavoitteena oli selvittää, mitä on potilasturvallisuus perioperatiivisessa hoitotyössä. Huomioon otettiin sekä potilaan fyysinen että psyykkinen turvallisuus. Potilasturvallisuutta tarkasteltiin pre-, intra-, ja postoperatiivisen hoitotyön vaiheissa. Tässä opinnäytetyössä postoperatiivisen turvallisuuden tarkastelu päättyi heräämöön. Opinnäytetyö toteutettiin systemaattisena kirjallisuuskatsauksena. Lähdeaineiston keruu suoritettiin maaliskuussa 2012 käyttäen Saima...

  7. Vaikuttamisen keinot yritysten vastuuviestinnässä

    OpenAIRE

    Heikura, Jenni

    2016-01-01

    ssä opinnäytetyössä käsiteltiin yritysten vastuuviestintää. Muodoltaan työ oli tutkimustyyppinen opinnäytetyö, jonka tarkoituksena oli tutkia vaikuttavuuden keinoja yritysten vastuuviestinnässä. Lisäksi työssä pohdittiin vastuuviestinnän keskeisiä ongelmia ja luotettavuutta. Opinnäytetyössä tarkasteltiin kahden yrityksen vastuuviestintää. Yrityksiksi valikoitiin UPM-Kymmene Oyj ja Stora Enso Oyj. Tutkimuksen aineistona käytettiin kyseisten yritysten vastuuviestintää niiden omilla intern...

  8. Empatiataitoa etsimässä

    OpenAIRE

    Erkamo, Merja; Ulkuniemi, Maria

    2013-01-01

    Tutkimuksella pyrittiin selvittämään minkälaisia keinoja varhaiskasvattajilla on päivähoidossa käytössä empatiataitojen tukemiseksi. Tutkimuksella kartoitettiin myös varhaiskasvattajien näkemyksiä siitä, olisiko empatiataitojen tukemiselle päivähoidossa kehittämistarvetta. Tutkimuksessa käytettiin tausta- ja tulkintateoriaa. Taustateoria käsittelee lapsen kehitystä ja empatian kehitystä. Tulkintateoriaa käytettiin tutkimusosiossa ja analysoinnin apuna. Tutkimus perustuu laadulliseen tutk...

  9. Toimintaterapia lastensuojelun perhetyössä

    OpenAIRE

    Myllykoski, Heli; Mertanen, Mira; Ollila, Kirsi

    2012-01-01

    Tämä opinnäytetyömme perustuu tutkimukselliseen kehittämistyöhön, joka toteutettiin yhteistyössä Merikratos Lastensuojelu Oy:n perhetyön kanssa. Merikratos Lastensuojelu Oy on yksityinen sosiaalialan yritys, joka tarjoaa kunnille ostopalveluna lastensuojelun avohuollon ja sijaishuollon tukitoimia. Perhetyö on lastensuojelun avohuollon tukitoimi perheille, joista on herännyt lastensuojelullinen huoli. Lastensuojelun palveluiden tulisi pystyä vastaamaan entistä paremmin ja tehokkaammin lasten j...

  10. MULTIWAVELENGTH OBSERVATIONS OF THE SS 433 JETS

    OpenAIRE

    Marshall, Herman L.; Canizares, Claude R.; Hillwig, Todd; Mioduszewski, Amy; Rupen, Michael; Schulz, Norbert S.; Nowak, Michael; Heinz, Sebastian

    2013-01-01

    We present observations of the SS 433 jets using the Chandra High Energy Transmission Grating Spectrometer with contemporaneous optical and Very Long Baseline Array observations. The X-ray and optical emission line regions are found to be related but not coincident as the optical line emission persists for days while the X-ray emission lines fade in less than 5000 s. The line Doppler shifts from the optical and X-ray lines match well, indicating that they are less than 3 × 10[superscript 14] ...

  11. Deceleration of SS433 radio jets

    CERN Document Server

    Panferov, A A

    2013-01-01

    The mildly relativistic jets of SS\\,433 are believed to inflate the surrounding supernova remnant W\\,50 depositing in its expansion possibly more than 99% of their kinetic energy (\\cite[1998]{Dub98}). Where and how this transformation of energy is curried out, it is not yet known. What can we learn from it that the jets decelerate and the deceleration is non-dissipative, i.e. radiatively dark. In this paper we unclose the observed deviations of the precessing radio jets of SS\\,433, within a few arcseconds from a jets source, from the ballistic track, described by the kinematic model, as a signature of the deceleration which, on other hand, issues from the jets colliding with ambient medium. For that we model kinematics of these colliding jets. The ram pressure on the jets is estimated from the observed profile of brightness of synchrotron radiation along the radio jets. We have found that to fit observed locus the radio jets should be decelerated and twisted, additionally to the precession twist, mostly withi...

  12. Multiwavelength Observations of the SS 433 Jets

    CERN Document Server

    Marshall, Herman L; Hillwig, Todd; Mioduszewski, Amy; Rupen, Michael; Schulz, Norbert S; Nowak, Michael; Heinz, Sebastian

    2013-01-01

    We present observations of the SS 433 jets using the Chandra High Energy Transmission Grating Spectrometer with contemporaneous optical and VLBA observations. The X-ray and optical emission line regions are found to be related but not coincident as the optical line emission persists for days while the X-ray emission lines fade in less than 5000 s. The line Doppler shifts from the optical and X-ray lines match well, indicating that they are less than 3e14 cm apart. The jet Doppler shifts show aperiodic variations that could result from shocks in interactions with the local environment. These perturbations are consistent with a change in jet direction but not jet speed. The proper motions of the radio knots match the kinematic model only if the distance to SS 433 is 4.5 +/- 0.2 kpc. Observations during eclipse show that the occulted emission is very hard, seen only above 2 keV and rising to comprise >50% of the flux at 8 keV. The soft X-ray emission lines from the jet are not blocked, constraining the jet lengt...

  13. The Research on rDNA ITS Sequences of Four Strains of Agaricus blazei%4个姬松茸菌株rDNA ITS序列比较

    Institute of Scientific and Technical Information of China (English)

    宋瑞清; 余江勇; 冀瑞卿; 胡伟; 李喜梅

    2007-01-01

    应用通用引物ITS1和ITS4从4个姬松茸(Agaricus blazei Murill.)菌株子实体中扩增出rDNA ITS序列(登录号分别为EF195648,EF471305,EF471306,EF471307),4菌株ITS序列长度分别为734bp、700bp、724bp和735bp.通过GenBank中BLAST,四个姬松茸菌株与A.brasiliensis(AJ884651)和A.blazei(AF161013)相似率最高,分别为:94%、99%、97%和98%.四个姬松茸菌株同源性较高,达93%以上,其中,ABM-B与ABM-D相似率最高,为97%;ABM-B与ABM-C相似率为96%,ABM-A与ABM-C、ABM-C与ABM-D相似率为94%,ABM-A与ABM-D相似率为93%.

  14. Kidney Function Indices in Mice after Long Intake of Agaricus brasiliensis Mycelia (=Agaricus blazei, Agaricus subrufescens Produced by Solid State Cultivation

    Directory of Open Access Journals (Sweden)

    Dalla S. O. Roberto

    2009-01-01

    Full Text Available Problem statement: Agaricus brasiliensis (=Agaricus blazei, Agaricus subrufescens or Sun mushroom has widespread use for potential health benefits such anti-tumor and immunomodulatory effects. Studies detected that others edible mushrooms affected renal metabolism and despite the widespread use of A. brasiliensis there are no studies that address biological effects on the renal function indices after their oral administration. Therefore, this study had as objective to verify the effects on kidney function indices after long intake of A. brasiliensis mycelium. Approach: Wheat grains was cultured during 18 days with Agaricus brasiliensis mycelium by solid state culture and used for chown formulation. Groups of female Swiss mice (20 per group were fed during 14 weeks with 100 and 50% of the formulated feed denominated A100 and A50, respectively. Control group received formulated chown with wheat grains without mycelium. The water intake and excreted urine volume; the physic chemistry analysis of the urine and the serum levels of glucose, proteins, urea, creatinin and uric acid was determined (Meditron Junior-Boehringer, reagent strips Combur 10; microscopy and ADVIA 1650 Bayer. Results: A100 and A50 groups ingested 19.1 and 15.8% more water compared to C group, respectively. The urine and serum analysis showed that the verified parameters remained invariables for all groups, including glucose levels, which resulted in a 10% reduction of A100 group, without statistical difference (p>0.05 Vs C. Conclusion/Recommendations: The prolonged intake of supplemented feed with A. brasiliensis mycelium didn’t result in indicative alterations in the kidney function indices. The preventive use of the mushroom did not show any deleterious effects on kidney; however complementary studies are necessary to guarantee complete safety; possible correlation between increase of urinary excretion and hypotensive effect

  15. Kinetics of polyphenol oxidase in Agaricus blazei%姬松茸多酚氧化酶的动力学特性

    Institute of Scientific and Technical Information of China (English)

    刘文聪

    2009-01-01

    测定了姬松茸不同部位的多酚氧化酶(PPO)活性,研究了不同pH、温度、底物浓度和抑制剂对PPO活性的影响,并建立酶促褐变反应动力学方程.结果表明:在邻苯二酚为底物的条件下,姬松茸PPO的最适pH为6.4,最适温度为30 ℃,米氏常数(K_m)为6.23×10~(-3) mol·L~(-1),最大反应速度(V_(max))为1.42 mol·L~(-1)·min~(-1);柠檬酸对PPO的抑制效果不明显,NaHSO_3和异V_C-Na对PPO均有较强的抑制作用,且有效抑制的含量分别为0.18%和0.21%.%The polyphenol oxidase (PPO) activities in different parts of Agaricus blazei Murrill were determined, the effects of pH value, temperature, substrate concentration and enzyme inhibitors on PPO were studied, too. The results showed that the optimum pH value and optimum temperature for PPO were pH 6.4 and 30 ℃, respectively, the Michaelis constant (K_m) and V_(max) of PPO with pyrocatechol substrate were 6.23 × 10~(-3) mol · L~(-1) and 1.42 mol · L~(-1) · min~(-1) respectively using catechol as substrates, and the reaction kinetic equation was established. The inhibition of citric acid on PPO was not satisfactory, NaHSO_3 and sodium d-isoascorbate could inhibit effectively, and the effective concentrations were 0.18% and 0.21% , respectively.

  16. Accretion disk structure in SS Cygni

    Science.gov (United States)

    Hessman, F. V.

    1987-02-01

    High-resolution coude observations of nonaxisymmetric line emission from the dwarf nova SS Cygni are presented. By subtracting the constant line component, the asymmetric line emission responsible for the observed phase shift between the absorption and emission line radial velocity curves can be isolated. The extra emission is a large fraction of the total line emission and extends to large velocities (of about 1500 km/sec). The phase stability of the emission demands a large-scale structure which is fixed in the frame of the binary. A magnetic origin of the excitation cannot be ruled out but is implausible. A simple explanation is that the accretion stream from the companion star is able to spill over the edge of the disk, introducing emission at noncircular velocities and most likely disturbing the upper layers of the accretion disk.

  17. MULTIWAVELENGTH OBSERVATIONS OF THE SS 433 JETS

    Energy Technology Data Exchange (ETDEWEB)

    Marshall, Herman L.; Canizares, Claude R.; Schulz, Norbert S.; Nowak, Michael [Kavli Institute for Astrophysics and Space Research, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139 (United States); Hillwig, Todd [Department of Physics and Astronomy, Valparaiso University, Valparaiso, IN 46383 (United States); Mioduszewski, Amy; Rupen, Michael [NRAO, P.O. Box 2, Socorro, NM 87801 (United States); Heinz, Sebastian, E-mail: hermanm@space.mit.edu, E-mail: crc@space.mit.edu, E-mail: nss@space.mit.edu, E-mail: mnowak@space.mit.edu, E-mail: todd.hillwig@valpo.edu, E-mail: amiodusz@nrao.edu, E-mail: mrupen@aoc.nrao.edu, E-mail: heinzs@astro.wisc.edu [Astronomy Department, 5408 Sterling Hall, University of Wisconsin, Madison, WI 53706 (United States)

    2013-09-20

    We present observations of the SS 433 jets using the Chandra High Energy Transmission Grating Spectrometer with contemporaneous optical and Very Long Baseline Array observations. The X-ray and optical emission line regions are found to be related but not coincident as the optical line emission persists for days while the X-ray emission lines fade in less than 5000 s. The line Doppler shifts from the optical and X-ray lines match well, indicating that they are less than 3 × 10{sup 14} cm apart. The jet Doppler shifts show aperiodic variations that could result from shocks in interactions with the local environment. These perturbations are consistent with a change in jet direction but not jet speed. The proper motions of the radio knots match the kinematic model only if the distance to SS 433 is 4.5 ± 0.2 kpc. Observations during eclipse show that the occulted emission is very hard, seen only above 2 keV and rising to comprise >50% of the flux at 8 keV. The soft X-ray emission lines from the jet are not blocked, constraining the jet length to ∼> 2 × 10{sup 12} cm. The base jet density is in the range 10{sup 10-13} cm{sup –3}, in contrast to our previous estimate based on the Si XIII triplet, which is likely to have been affected by UV de-excitation. There is a clear overabundance of Ni by a factor of about 15 relative to the solar value, which may have resulted from an unusual supernova that formed the compact object.

  18. Multiwavelength Observations of the SS 433 Jets

    Science.gov (United States)

    Marshall, Herman L.; Canizares, Claude R.; Hillwig, Todd; Mioduszewski, Amy; Rupen, Michael; Schulz, Norbert S.; Nowak, Michael; Heinz, Sebastian

    2013-09-01

    We present observations of the SS 433 jets using the Chandra High Energy Transmission Grating Spectrometer with contemporaneous optical and Very Long Baseline Array observations. The X-ray and optical emission line regions are found to be related but not coincident as the optical line emission persists for days while the X-ray emission lines fade in less than 5000 s. The line Doppler shifts from the optical and X-ray lines match well, indicating that they are less than 3 × 1014 cm apart. The jet Doppler shifts show aperiodic variations that could result from shocks in interactions with the local environment. These perturbations are consistent with a change in jet direction but not jet speed. The proper motions of the radio knots match the kinematic model only if the distance to SS 433 is 4.5 ± 0.2 kpc. Observations during eclipse show that the occulted emission is very hard, seen only above 2 keV and rising to comprise >50% of the flux at 8 keV. The soft X-ray emission lines from the jet are not blocked, constraining the jet length to >~ 2 × 1012 cm. The base jet density is in the range 1010-13 cm-3, in contrast to our previous estimate based on the Si XIII triplet, which is likely to have been affected by UV de-excitation. There is a clear overabundance of Ni by a factor of about 15 relative to the solar value, which may have resulted from an unusual supernova that formed the compact object.

  19. Molecular dynamics simulations of a single stranded (ss) DNA

    CERN Document Server

    Chatterjee, Subhasish; Thakur, Siddarth; Burin, Alexander

    2012-01-01

    The objective of the present study was to develop an understanding of short single-stranded DNA (ssDNA) to assist the development of new DNA-based biosensors. A ssDNA model containing twelve bases was constructed from the 130-145 codon sequence of the p53 gene. Various thermodynamic macroscopic observables such as temperature, energy distributions, as well as root mean square deviation (RMSD) of the nucleic acid backbone of the ssDNA were studied using molecular dynamics (MD) simulations. The AMBER program was used for building the structural model of the ssDNA, and atomistic MD simulations in three different ensembles were carried out using the NAMD program. The microcanonical (NVE), conical (NVT) and isobaric-isothermal (NPT) ensembles were employed to compare the equilibrium characteristics of ssDNA in aqueous solutions. Our results indicate that the conformational stability of the ssDNA is dependent on the thermodynamic conditions.

  20. Tukiasumisyksikössä asuvien maahanmuuttajanuorten kokemukset kotoutumisesta

    OpenAIRE

    Haataja, Auri-Elina; Tampio, Sanna

    2012-01-01

    Haataja, Auri & Tampio, Sanna. Tukiasumisyksikössä asuvien maahanmuuttajanuorten kokemukset kotoutumisesta. Diak Pohjoinen, Oulu, kevät 2012, 61 s., 1 liite. Diakonia-ammattikorkeakoulu, Sosiaalialan koulutusohjelma, Diakonisen sosiaalityön suuntautumisvaihtoehto, sosionomi (AMK) + diakoni. Opinnäytetyön tarkoituksena oli kuvata tukiasumisyksikössä asuvien maahanmuuttajanuorten kokemuksia kotoutumisesta Pudasjärvelle. Opinnäytetyön tavoitteena oli tuottaa tietoa tukiasumisyksikössä asu...

  1. The reproducible radio outbursts of SS Cygni

    Science.gov (United States)

    Russell, T. D.; Miller-Jones, J. C. A.; Sivakoff, G. R.; Altamirano, D.; O'Brien, T. J.; Page, K. L.; Templeton, M. R.; Körding, E. G.; Knigge, C.; Rupen, M. P.; Fender, R. P.; Heinz, S.; Maitra, D.; Markoff, S.; Migliari, S.; Remillard, R. A.; Russell, D. M.; Sarazin, C. L.; Waagen, E. O.

    2016-08-01

    We present the results of our intensive radio observing campaign of the dwarf nova SS Cyg during its 2010 April outburst. We argue that the observed radio emission was produced by synchrotron emission from a transient radio jet. Comparing the radio light curves from previous and subsequent outbursts of this system (including high-resolution observations from outbursts in 2011 and 2012) shows that the typical long and short outbursts of this system exhibit reproducible radio outbursts that do not vary significantly between outbursts, which is consistent with the similarity of the observed optical, ultraviolet and X-ray light curves. Contemporaneous optical and X-ray observations show that the radio emission appears to have been triggered at the same time as the initial X-ray flare, which occurs as disc material first reaches the boundary layer. This raises the possibility that the boundary region may be involved in jet production in accreting white dwarf systems. Our high spatial resolution monitoring shows that the compact jet remained active throughout the outburst with no radio quenching.

  2. The reproducible radio outbursts of SS Cygni

    CERN Document Server

    Russell, T D; Sivakoff, G R; Altamirano, D; O'Brien, T J; Page, K L; Templeton, M R; Koerding, E G; Knigge, C; Rupen, M P; Fender, R P; Heinz, S; Maitra, D; Markoff, S; Migliari, S; Remillard, R A; Russell, D M; Sarazin, C L; Waagen, E O

    2016-01-01

    We present the results of our intensive radio observing campaign of the dwarf nova SS Cyg during its 2010 April outburst. We argue that the observed radio emission was produced by synchrotron emission from a transient radio jet. Comparing the radio light curves from previous and subsequent outbursts of this system (including high-resolution observations from outbursts in 2011 and 2012) shows that the typical long and short outbursts of this system exhibit reproducible radio outbursts that do not vary significantly between outbursts, which is consistent with the similarity of the observed optical, ultraviolet and X-ray light curves. Contemporaneous optical and X-ray observations show that the radio emission appears to have been triggered at the same time as the initial X-ray flare, which occurs as disk material first reaches the boundary layer. This raises the possibility that the boundary region may be involved in jet production in accreting white dwarf systems. Our high spatial resolution monitoring shows th...

  3. Russia turns to U.N. to condemn Waffen SS

    Index Scriptorium Estoniae

    2004-01-01

    Venemaa algatusel võeti Genfis ÜRO Inimõiguste komisjoni istungil vastu resolutsioon, mis mõistab hukka omaaegsete Waffen-SS-i võitlejate õigustamise ja ülistamise, SS-laste auks mälestusmärkide püstitamise, nende rongkäigud ja muud sedalaadi üritused

  4. Human bulbar conjunctival hemodynamics in hemoglobin SS and SC disease.

    Science.gov (United States)

    Wanek, Justin; Gaynes, Bruce; Lim, Jennifer I; Molokie, Robert; Shahidi, Mahnaz

    2013-08-01

    The known biophysical variations of hemoglobin (Hb) S and Hb C may result in hemodynamic differences between subjects with SS and SC disease. The purpose of this study was to measure and compare conjunctival hemodynamics between subjects with Hb SS and SC hemoglobinopathies. Image sequences of the conjunctival microcirculation were acquired in 9 healthy control subjects (Hb AA), 24 subjects with SC disease, and 18 subjects with SS disease, using a prototype imaging system. Diameter (D) and blood velocity (V) measurements were obtained in multiple venules of each subject. Data were categorized according to venule caliber by averaging V and D for venules with diameters less than (vessel size 1) or greater than (vessel size 2) 15 µm. V in vessel size 2 was significantly greater than V in vessel size 1 in the AA and SS groups (P ≥ 0.009), but not in the SC group (P = 0.1). V was significantly lower in the SC group as compared to the SS group (P = 0.03). In AA and SS groups, V correlated with D (P ≤ 0.005), but the correlation was not statistically significant in the SC group (P = 0.08). V was inversely correlated with hematocrit in the SS group for large vessels (P = 0.03); however, no significant correlation was found in the SC group (P ≥ 0.2). Quantitative assessment of conjunctival microvascular hemodynamics in SS and SC disease may advance understanding of sickle cell disease pathophysiology and thereby improve therapeutic interventions.

  5. Mechanochemical regulations of RPA's binding to ssDNA

    Science.gov (United States)

    Chen, Jin; Le, Shimin; Basu, Anindita; Chazin, Walter J.; Yan, Jie

    2015-03-01

    Replication protein A (RPA) is a ubiquitous eukaryotic single-stranded DNA (ssDNA) binding protein that serves to protect ssDNA from degradation and annealing, and as a template for recruitment of many downstream factors in virtually all DNA transactions in cell. During many of these transactions, DNA is tethered and is likely subject to force. Previous studies of RPA's binding behavior on ssDNA were conducted in the absence of force; therefore the RPA-ssDNA conformations regulated by force remain unclear. Here, using a combination of atomic force microscopy imaging and mechanical manipulation of single ssDNA tethers, we show that force mediates a switch of the RPA bound ssDNA from amorphous aggregation to a much more regular extended conformation. Further, we found an interesting non-monotonic dependence of the binding affinity on monovalent salt concentration in the presence of force. In addition, we discovered that zinc in micromolar concentrations drives ssDNA to a unique, highly stiff and more compact state. These results provide new mechanochemical insights into the influences and the mechanisms of action of RPA on large single ssDNA.

  6. Extending OmpSs to support dynamic programming

    OpenAIRE

    Maroñas Bravo, Marcos

    2015-01-01

    [CASTELLÀ] Tras un breve resumen sobre programación dinámica y OmpSs, este documento muestra como OmpSs ofrece soporte para memoización y los resultados obtenidos. Se detallan las extensiones del modelo, del compilador Mercurium y del runtime Nanos++ realizadas para soportar memoización.

  7. The anti-tumorigenic mushroom Agaricus blazei Murill enhances IL-1β production and activates the NLRP3 inflammasome in human macrophages.

    Directory of Open Access Journals (Sweden)

    Tsung-Teng Huang

    Full Text Available Agaricus blazei Murill (AbM has been reported to possess immune activity against tumors and infections through stimulation of mononuclear phagocytes. Recently, AbM extract was shown to induce the production of the pro-inflammatory cytokine, interleukin-1β (IL-1β, in human monocytes. IL-1β is a key pro-inflammatory cytokine produced by activated macrophages and monocytes and its secretion is strictly controlled by the inflammasome. The purpose of this study is to investigate the effect of AbM water extracts on the regulation of IL-1β production and activation of the NLRP3 inflammasome in human THP-1 macrophages. The NLRP3 inflammasome consists of an NLRP3 receptor, an adaptor protein called ASC, and the inflammatory protease, caspase-1. Typically, stimulation of immune cells with microbial products results in production of pro-IL-1β, but a second stress-related signal activates the inflammasome and caspase-1, leading to processing and secretion of IL-1β. Our results show that AbM enhances transcription of IL-1β and triggers NLRP3 inflammasome-mediated IL-1β secretion in human THP-1 macrophages. AbM-mediated IL-1β secretion was markedly reduced in macrophages deficient in NLRP3 and ASC, demonstrating that the NLRP3 inflammasome is essential for AbM-induced IL-1β secretion. In addition, caspase-1 was activated and involved in proteolytic cleavage and secretion of IL-1β in AbM-treated macrophages. AbM-mediated IL-1β secretion also decreased in cells treated with cathepsin B inhibitor, suggesting that AbM can induce the release of cathepsin B. Furthermore, our data show that AbM-induced inflammasome activation requires the release of ATP, binding of extracellular ATP to the purinergic receptor P2X(7, the generation of reactive oxygen species, and efflux of potassium. Taken together, these findings reveal that AbM activates the NLRP3 inflammasome via multiple mechanisms, resulting in the secretion of IL-1β.

  8. Turboahtimen mitoitus polttomoottorikäytössä

    OpenAIRE

    Rantanen, Jyri

    2014-01-01

    Tämä opinnäytetyö käsittelee turboahtimen mitoittamiseen liittyvää teoriaa, sekä käytäntöä polttomoottorikäyttöisissä autoissa. Työssä käydään lävitse tarvittavat laskukaavat, joiden avulla saadaan apua oikeanlaisen ahtimen valintaan. Tässä työssä keskitytään enemmän ahtimen valintaan moottorin virityskäytössä, kuin suurimman mahdollisen käytettävyyden sekä polttoainetalouden saavuttamiseen. Opinnäytetyössä lasketaan teoreettisilla kaavoilla erään ahtimen sopivuus tietylle autolle. Lopuks...

  9. New rhodomycin analogs, SS-288A and SS-288B, produced by a Streptomyces violaceus A262 mutant.

    Science.gov (United States)

    Saito, S; Katsuda, Y; Johdo, O; Yoshimoto, A

    1995-01-01

    Two new rhodomycin metabolites, SS-288A and SS-288B, were specifically produced by a blocked mutant obtained from Streptomyces violaceus A262 and were respectively identified as 7,10-di(O-rhodosaminyl-deoxyfucosyl-deoxyfucosyl)-beta -rhodomycinone and -beta-isorhodomycinone. PMID:7765964

  10. Toivo sairaanhoitajan työssä : Sairaanhoitajien kokemuksia toivosta hoitotyössä

    OpenAIRE

    Lehtinen, Katri

    2011-01-01

    Tämän opinnäytetyön tarkoituksena oli kuvata, minkä sisällön sairaanhoitajat antoivat käsitteelle toivo ja miten he toivon kokivat. Opinnäytetyön tavoitteena oli tuoda näkyväksi sairaanhoitajien kokemukset toivosta omassa työssä. Saadun tiedon perusteella toivo on lähtökohta sairaanhoitajan työn toteutumiselle ja potilaan toipumiselle sairaudestaan. Opinnäytetyö toteutui kvalitatiivisen tutkimusmenetelmän mukaisesti. Aineisto kerättiin haastattelemalla sairaanhoitajia. Sain tietoa haastat...

  11. Langaton tiedonsiirto sulautetussa järjestelmässä

    OpenAIRE

    Rosila, Ville

    2014-01-01

    ssä opinnäytetyöraportissa käsitellään langattoman tiedonsiirron toteuttamista erääseen sulautettuun järjestelmään. Työn tärkeimpänä tavoitteena oli saada langaton tiedonsiirto toimimaan järjestelmässä tehokkaasti ja luotettavasti. Toisena tavoitteena oli toteuttaa jokin käytännönläheinen esimerkkisovellus, jossa langattomasta tiedonsiirrosta on hyötyä. Raportissa perehdytään työssä käytettyihin komponentteihin ja niiden oleellisimpiin teknisiin ominaisuuksiin. Komponenttien lisäksi esitell...

  12. Laitteiden testauksessa kulutettu energia Vacon Oyj:ssä

    OpenAIRE

    Mustonen, Tero

    2013-01-01

    Vacon Oyj on osana energiansäästökampanjaa, jonka tavoitteena on vähentää energiankulutusta 20 % vuoteen 2016 mennessä.Tässä työssä tutkitaan Vacon Oyj:n taajuusmuuttajien ja inverttereiden testaamiseen kuluvien energioiden suhteita. Työssä mitattiin testattavien laitteiden verkosta otettavan energian ja testauspiirissä kierrätettävän energian suhdetta eri testauskonsepteissa. Työhön kuului mittauslaitteistojen käyttöönotto sekä uusien suunnittelu ja rakentaminen. Tulokset kirjattiin ylös työ...

  13. Brucella T4SS: the VIP pass inside host cells.

    Science.gov (United States)

    Lacerda, Thais Lourdes Santos; Salcedo, Suzana Pinto; Gorvel, Jean-Pierre

    2013-02-01

    For many Gram-negative bacteria, like Brucella, the type IV secretion system (T4SS) has a critical role in bacterial virulence. In Brucella, the VirB T4SS permits the injection of bacterial effectors inside host cells, leading to subversion of signaling pathways and favoring bacterial growth and pathogenesis. The virB operon promoter is tightly regulated by a combination of transcriptional activators and repressors that are expressed according to the environmental conditions encountered by Brucella. Recent advances have shed light on the Brucella T4SS regulatory mechanisms and also its substrates. Characterization of the targets and functions of these translocated effectors is underway and will help understand the role of the T4SS in the establishment of a replication niche inside host cells.

  14. Hybrid MPI/StarSs - a case study

    OpenAIRE

    Gracia, Jose; Niethammer, Christoph; Hasert, Manuel; Brinkmann, Steffen; Keller, Rainer; Glass, Colin W.

    2012-01-01

    Hybrid parallel programming models combining distributed and shared memory paradigms are well established in high-performance computing. The classical prototype of hybrid programming in HPC is MPI/OpenMP, but many other combinations are being investigated. Recently, the data-dependency driven, task parallel model for shared memory parallelisation named StarSs has been suggested for usage in combination with MPI. In this paper we apply hybrid MPI/StarSs to a Lattice-Boltzmann code. In particul...

  15. Architecture of SS7-IP Interworking%SS7与IP互通体系结构

    Institute of Scientific and Technical Information of China (English)

    罗国明; 寇化栋

    2004-01-01

    随着电信网和互联网融合技术的发展,传统电路交换技术将逐步转向分组技术.下一代电信网是基于IP的网络,SS7及呼叫控制原理是软交换控制系统的重要组成部分.首先介绍了SS7与IP互通的概念模型和SIGTRAN协议体系,对4种信令适配子层协议和SCTP进行了详细的阐述和分析.给出了3种主要的互通模式,对如何实现基于IP的SS7传送和构建IP SS7网的相关技术进行了研究.

  16. 姬松茸60Co辐射新菌株J3营养成分与农药残留分析%Analysis on Nutrients and Pesticide Residues in Strain J3 Agaricus blazei Murill Irradiated by 60Co

    Institute of Scientific and Technical Information of China (English)

    翁伯琦; 江枝和; 肖淑霞; 雷锦桂; 王义祥; 唐翔虬

    2011-01-01

    通过常规生产床栽,对比研究姬松茸Co辐射新菌株J和原菌株J在营养品质以及重金属含量和农药残留情况.结果表明,姬松茸Co辐射新菌株J子实体中鲜味氨基酸总量(56.5 g·kg)、甜味氨基酸总量(52.4g·kg)、硫氨基酸总量(15.8 g·kg)、支链氨基酸总量(38.1 g·kg)、芳香族氨基酸总量(16.1 g·kg)、儿童氨基酸总量(17.8 g·kg)、必需氨基酸总量(95 g·kg),分别占氨基酸总量(221.7 g·kg)的25.48%、23.64%、7.13%、17.19%、7.26%、8.03%和42.85%,分别比姬松茸J高28.12%、12.93%、0%、14.41%、12.59%、16.34%和11.76%.J的多不饱和脂肪酸(76.15%)和不饱和脂肪酸(77.55%)分别比姬松茸原菌株J高4.39%和3.82%.J子实体含镉(3.86 mg·kg)、汞(0.42 mg·kg)和砷(0.09 mg·kg),也分别比原菌株J低45.86%、32.25%和18.18%;其铅含量符合国家食用菌卫生标准GB 7096-2003;砷含量符合国家绿色食品食用菌NY/T749-2003的标准.子实体中联苯菊酯、溴氰菊酯、百菌清、六六六、多菌灵、阿维菌素、甲基托布津、甲胺磷、毒死蜱、滴滴涕和二氧化硫含量均符合国家绿色食品食用菌NY/T 749-2003的标准.%A garicus blazei Murill as rare edible fungi was adored by more and more customers.But with the increasing attention of food hygiene quality, it was important guidance of the safety production of Agaricus blazei to understand the health status of Agaricus blazei in the actual cultivation.This paper studied the difference in terms of nutritional quality, heavy metal contents and pesticide residues between strain J3 and J1.The results showed that the contents of delicious amino acid(56.5 g· kg-1), sweet amino acid(52.4 g· kg-1), sulfur amino acid( 15.8 g·kg-1), branched chain amino acid(38.1 g· kg-1), aromatic amino acid( 16.1 g·kg-1), children amino acid( 17.8 g·kg-1), necessary amino acid (95.0 g·kg-1)in the fruit bodies ofAgaricus blazei J3, occupied 25.48%, 23.64%, 7.13%, 17.19%, 7.26

  17. SS-mPMG and SS-GA: tools for finding pathways and dynamic simulation of metabolic networks.

    Science.gov (United States)

    Katsuragi, Tetsuo; Ono, Naoaki; Yasumoto, Keiichi; Altaf-Ul-Amin, Md; Hirai, Masami Y; Sriyudthsak, Kansuporn; Sawada, Yuji; Yamashita, Yui; Chiba, Yukako; Onouchi, Hitoshi; Fujiwara, Toru; Naito, Satoshi; Shiraishi, Fumihide; Kanaya, Shigehiko

    2013-05-01

    Metabolomics analysis tools can provide quantitative information on the concentration of metabolites in an organism. In this paper, we propose the minimum pathway model generator tool for simulating the dynamics of metabolite concentrations (SS-mPMG) and a tool for parameter estimation by genetic algorithm (SS-GA). SS-mPMG can extract a subsystem of the metabolic network from the genome-scale pathway maps to reduce the complexity of the simulation model and automatically construct a dynamic simulator to evaluate the experimentally observed behavior of metabolites. Using this tool, we show that stochastic simulation can reproduce experimentally observed dynamics of amino acid biosynthesis in Arabidopsis thaliana. In this simulation, SS-mPMG extracts the metabolic network subsystem from published databases. The parameters needed for the simulation are determined using a genetic algorithm to fit the simulation results to the experimental data. We expect that SS-mPMG and SS-GA will help researchers to create relevant metabolic networks and carry out simulations of metabolic reactions derived from metabolomics data.

  18. Performance of DMFC with SS 316 bipolar/end plates

    Science.gov (United States)

    Padhy, Biswa R.; Reddy, Ramana G.

    This work mainly emphasizes the development of new materials and design for a bipolar/end plate in a direct methanol fuel cell (DMFC). According to the DOE requirements, preliminary studies show that SS 316 (Stainless Steel 316) is a suitable candidate. Several flow field designs were studied and a modified serpentine design was proposed. SS 316 end plates were fabricated with an intricate modified serpentine flow field design on it. The performance of a single stack DMFC with SS 316 end plates were studied with different operational parameters. A long-term test was carried out for 100 h with recycling the methanol and the contaminants in the MEA were characterized. The stack efficiency is found to be 51% and polarization losses are discussed. SS 316 with low permeability resulted in an increased pressure drop across the flow field, which increased the fuel cell performance. The use of SS 316 as bipolar plate material will reduce the machining cost as well as volume of the fuel cell stack.

  19. Hybrid MPI/StarSs - a case study

    CERN Document Server

    Gracia, Jose; Hasert, Manuel; Brinkmann, Steffen; Keller, Rainer; Glass, Colin W

    2012-01-01

    Hybrid parallel programming models combining distributed and shared memory paradigms are well established in high-performance computing. The classical prototype of hybrid programming in HPC is MPI/OpenMP, but many other combinations are being investigated. Recently, the data-dependency driven, task parallel model for shared memory parallelisation named StarSs has been suggested for usage in combination with MPI. In this paper we apply hybrid MPI/StarSs to a Lattice-Boltzmann code. In particular, we present the hybrid programming model, the benefits we expect, the challenges in porting, and finally a comparison of the performance of MPI/StarSs hybrid, MPI/OpenMP hybrid and the original MPI-only versions of the same code.

  20. Interaction of Rheumatoid Factor with Immobilized ss-DNA

    Institute of Scientific and Technical Information of China (English)

    WANG Lian-yong; LU Jing; YU Yao-ting

    2004-01-01

    Rheumatoid factors(RFs) are the characteristic autoantibodies of rheumatoid arthritis. Recent researches in our laboratory showed that the immobilized single-stranded DNA(ss-DNA) immunoadsorbent can selectively remove RFs from the serum of patients. In the present paper are studied the modification of argininine, tryptophan, lysine residues and carboxyl terminus of IgGRF, which was separated from patients′ serum, with 1,2-cyclohexanedione(CHD), N-bromosuccinimide(NBS), pyridoxal 5′-phosphate(PP) and 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide(EDC) respectively, and their effects on the adsorption capacity of the immobilized ss-DNA immunoadsorbent for IgGRF. After the specific modification, the corresponding adsorption capacities of the adsorbents were changed from 48%, 46%, 44% and 54% to 84%, 14%, 21% and 81%, respectively. These results indicate that the electrostatic or ionic-bonding is essential for the interaction between ss-DNA and IgGRF.

  1. 27 CFR 24.47 - Execution of IRS Form SS-4.

    Science.gov (United States)

    2010-04-01

    ... 27 Alcohol, Tobacco Products and Firearms 1 2010-04-01 2010-04-01 false Execution of IRS Form SS-4... Number § 24.47 Execution of IRS Form SS-4. (a) Preparation. The application on IRS Form SS-4, together... center as instructed on the Form SS-4. (b) Signature. The application will be signed by: (1)...

  2. 75 FR 10017 - Proposed Collection; Comment Request for Form SS-8

    Science.gov (United States)

    2010-03-04

    ... Internal Revenue Service Proposed Collection; Comment Request for Form SS-8 AGENCY: Internal Revenue...(c)(2)(A)). Currently, the IRS is soliciting comments concerning Form SS-8, Determination of Worker... Employment Taxes and Income Tax Withholding. OMB Number: 1545-0004. Form Number: SS-8. Abstract: Form SS-8...

  3. 78 FR 23981 - Proposed Collection; Comment Request for Form SS-8

    Science.gov (United States)

    2013-04-23

    ... Internal Revenue Service Proposed Collection; Comment Request for Form SS-8 AGENCY: Internal Revenue...(c)(2)(A)). Currently, the IRS is soliciting comments concerning Form SS-8, Determination of Worker... Employment Taxes and Income Tax Withholding. OMB Number: 1545-0004. Form Number: SS-8. Abstract: Form SS-8...

  4. 76 FR 34296 - Proposed Collection; Comment Request for Forms 1040-PR and 1040-SS

    Science.gov (United States)

    2011-06-13

    ... Internal Revenue Service Proposed Collection; Comment Request for Forms 1040-PR and 1040- SS AGENCY... dito Tributario Adicional por Hijos para Residentes Bona Fide de Puerto Rico) and Form 1040-SS, U.S... Rico). OMB Number: 1545-0090. Form Number: Form 1040-SS. Abstract: Form 1040-SS, is used by...

  5. e-VLBI observations of SS 433 in outburst

    Science.gov (United States)

    Tudose, V.; Paragi, Z.; Trushkin, S.; Soleri, P.; Fender, R.; Garrett, M.; Spencer, R.; Rushton, A.; Burgess, P.; Kunert-Bajraszewska, M.; Pazderski, E.; Borkowski, K.; Hammargren, R.; Lindqvist, M.; Maccaferri, G.

    2008-11-01

    We have observed the X-ray binary SS 433 on November 6, 2008 between 13:48-18:35 UT at 5 GHz with the European VLBI Network (EVN) using the e-VLBI technique. The radio telescopes participating in the experiment were: Medicina, Onsala 25m, Torun, Jodrell Bank MkII and Cambridge. The X-ray binary SS 433 is in outburst. Trushkin & Nizhelskij (ATel #1819) reported a major flare already active during the RATAN-600 observations in the 1-22 GHz band on 2008 October 28.

  6. ICT:n rooli toimitusketjuyhteistyössä

    OpenAIRE

    Fagerström, Marko

    2013-01-01

    Opinnäytetyö on osa SULOIN-hanketta, jossa haetaan uusia toimintamalleja elintarviketeollisuuden jakelukanavien kehittämiseen. Opinnäytetyössä tutkittiin, mikä on ICT:n rooli toimitusketjuyhteistyössä, ja minkälainen vaikutus sillä on jakelukanavan markkina- ja neuvotteluvoimiin. Tutkimusstrategiana käytettiin kvalitatiivista tutkimusta, ja pääasiallinen tutkimusmenetelmä oli kohdeyrityksissä tehdyt teemahaastattelut. Yrityksiä, joissa haastattelut tehtiin, oli kolme. Näistä kaksi on elin...

  7. Laulaen iloa saamme : musiikki vapaaehtoistyössä

    OpenAIRE

    Herranen, Linda

    2011-01-01

    Vuoden 2009 lopulla Eläkeliitto otti yhteyttä ammattikorkeakouluumme ja kysyi halukkaita tekemään opinnäytetyötä Sävel soikoon –hankkeesta. Hankkeen idea on kouluttaa vapaaehtoisina toimijoita pitämään musiikkituokioita vanhainkodeissa, sairaaloissa ja yksityiskodeissa. Opinnäytetyössäni tarkastelin vapaaehtoistyössä käytettävää musiikkia. Tarkoituksena oli selvittää, millä perusteella vapaaehtoiset valitsevat musiikkituokiossa käytettävät kappaleet, mitä kappaleita vapaaehtoiset käyttävät mi...

  8. Hoitajien kokemuksia omahoitajuudesta psykiatrisessa hoitotyössä

    OpenAIRE

    Heinonen, Noora; Viita-aho, Jori

    2014-01-01

    Oulun seudun ammattikorkeakoulu Hoitotyön koulutusohjelma Tekijät: Noora Heinonen ja Jori Viita-aho Opinnäytetyön nimi: Hoitajien kokemuksia omahoitajuudesta psykiatrisessa hoitotyössä Työn ohjaaja: Liisa Kiviniemi Valmistumisaika: Kevät 2014 Sivumäärä: 37 Opinnäytetyön tarkoituksena oli kuvailla hoitajien kokemuksia omahoitajuudesta psykiatrisessa hoitotyössä. Opinnäytetyön tavoitteena oli tuottaa kokemusperäistä tietoa omahoitajuuden toteuttamisesta ja sisällö...

  9. Ammatillisen vuorovaikutuksen tukeminen ohjaustyössä

    OpenAIRE

    Lappalainen, Sari; Kurppa, Jatta

    2016-01-01

    Tämä opinnäytetyö on toiminnallinen opinnäytetyö. Sen aiheena oli ammatillisen vuorovaikutuksen tukeminen ohjaustyössä. Tavoitteena oli herättää ajatuksia ja laajentaa näkökulmaa ammatillisissa vuorovaikutustilanteissa. Opinnäytetyön teoreettiseksi viitekehykseksi valittiin teorioita, jotka olennaisesti vaikuttavat ammatillisen vuorovaikutuksen toteutumiseen ohjaustyössä. Opinnäytetyön toimeksiantajana toimi AMF Vastaanottopalvelut. Toiminnallinen osuus toteutettiin koko työyhteisölle yh...

  10. Toimitusjohtajan rooli ja asema perheyhtiössä

    OpenAIRE

    Fontell, Marleena

    2015-01-01

    Toimitusjohtajan rooli ja asema ovat usein epäselviä. Erityisesti pienissä perheyrityksissä toimitusjohtajan rooli voi olla epämääräinen, tai sitä ei ole erikseen määritelty. Opinnäytetyössä tarkastellaan toimitusjohtajan roolia ja asemaa perheyrityksessä X, jossa on tapahtunut sukupolvenvaihdos. Tutkimuksessa haluttiin tuoda esiin sopimusoikeudellinen puoli, ovatko toimitusjohtajan toimet lainsäädännön mukaisia vai onko hän enemmän työntekijän roolissa. Opinnäytetyössä tutkittiin, mikä o...

  11. YouTube osuuskaupan markkinointiviestinnässä

    OpenAIRE

    Lindevall, Tytti

    2014-01-01

    Tämän opinnäytetyön tavoitteena on luoda toimeksiantaja Suur-Seudun Osuuskauppa SSO:lle suunnitelma YouTuben hyödyntämiseksi markkinointiviestinnässään. Työn avulla on tarkoitus löytää keino, miten SSO voisi hyödyntää YouTubea markkinointiviestinnässään ja mitä asioita tulisi huomioida omaa kanavaa perustettaessa. Tarkoituksena on tuottaa raportti mahdollisista käyttötarkoituksista sekä ohjeistus oman kanavan perustamiseen ja hallinnointiin liittyvistä asioista. Työn alkupuolella taustoite...

  12. Sosiaalinen vahvistaminen avoimessa nuorisotilatyössä

    OpenAIRE

    Santanen, Lotta-Julia; Nieminen, Katri

    2013-01-01

    Toiminnallinen opinnäytetyö toteutettiin yhteistyössä Jyväskylän kaupungin nuorisotoimen ja Humanistisen ammattikorkeakoulun kanssa syksyllä 2013. Opinnäytetyön tavoitteena oli selvittää millaista on sosiaalinen vahvistaminen avoimessa tilatoiminnassa sekä mikä on työntekijän rooli sosiaalisessa vahvistamisessa uudella tilalla. Tutkimusmenetelmiksi kehiteltiin kaksi sosiaalista vahvistamista tukevaa toiminnallista menetelmää, joita testattiin käytännössä kahdella eri Jyväskylän kaupungin...

  13. The development of a quantitative assay for the detection of anti-Ro/SS-A and anti-LA/SS-B autoantibodies using purified recombinant proteins.

    Science.gov (United States)

    Veldhoven, C H; Meilof, J F; Huisman, J G; Smeenk, R J

    1992-07-01

    A characteristic of patients with autoimmune diseases such as Sjögren's syndrome and systemic lupus erythematosus is the presence of anti-Ro/SS-A and anti-La/SS-B autoantibodies in their circulation. In order to investigate specific autoantibody levels in the sera of these patients quantitative assays for the detection of both anti-Ro/SS-A and anti-La/SS-B reactivity were developed. Ro/SS-A (60 kDa) and La-SS-B (50 kDa) cDNAs were cloned and expressed in E. coli as non-fusion proteins. These were purified to homogeneity using two different purification protocols. With these recombinant antigens, specific enzyme-linked immunosorbent assays (ELISAs) were developed. 40 sera positive for anti-Ro/SS-A autoantibodies in counterimmunoelectrophoresis (CIE) were tested in both the Ro/SS-A and La/SS-B ELISA. Activity values reproducibly ranged from 1536 to 120,000 U in the Ro/SS-A ELISA and from 763 to 2,500,000 U in the La/SS-B ELISA. The suitability of these ELISAs as screening assays was further investigated by testing 200 sera sent to our laboratory for routine detection of autoantibodies to extractable nuclear antigen (ENA: anti-Sm, anti-RNP, anti-Ro/SS-A and anti-La/SS-B). Both ELISAs showed a high sensitivity and specificity (Ro/SS-A ELISA 85% and 94%, La/SS-B ELISA 100% and 98% respectively), when compared to the standard assays, the RNA-precipitation assay and the HeLa immunoblotting test. From these data we conclude that a quantitative analysis of both anti-Ro/SS-A and anti-La/SS-B autoantibodies is now possible using purified recombinant non-fusion proteins. For screening purposes the La/SS-B ELISA showed a great improvement in sensitivity for the detection of anti-La/SS-B activity in comparison to the La/SS-B CIE, while the Ro/SS-A ELISA almost equalled the performance of the Ro/SS-A CIE.

  14. Molecular Mapping of Sterility QTLs qSS-3, qSS-6a and qSS-7 as Single Mendelian Factors via NIL strategy

    Institute of Scientific and Technical Information of China (English)

    LI Jing; XU Peng; ZHOU Jia-wu; HU Feng-yi; DENG Xian-neng; CHEN Zhi-wei; TAO Da-yun

    2011-01-01

    Hybrid sterility between Oryza glaberrima and O.sativa seriously hampers the introgression of favorable genes from each other.In order to further understand this issue,identification and isolation of hybrid sterility QTLs as single Mendelian factors are an effective strategy.A genetic map was constructed using a BC1F1 population derived from a cross between an O.sativa japonica cultivar and an O.glaberrima accession.Four main-effect QTLs for pollen sterility were detected in the BC1 F1.Five BC8F1 advanced backcross populations were developed via successive backcrosses based on phenotype and molecular selections.The BC8F1 populations showed bimodal distribution for pollen fertility and could be classified into semi-sterile and fertile types,fitting single Mendilian factor inheritance ratios.Three QTLs detected in the BC1F1 corresponding to qSS-3,qSS-6a and qSS-7 were mapped on chromosomes 6,3 and 7,respectively,as single Mendilian factors.

  15. Turvallisuuteen liittyvien lupaehtojen noudattaminen maantiellä tehtävässä työssä

    OpenAIRE

    Ahola, Mikael

    2011-01-01

    Maantiellä tehtävä työ on luvanvaraista työtä, jota hallinnoivat tienpitoviran-omaisena paikalliset elinkeino-, liikenne- ja ympäristökeskukset. Opinnäytetyön tarkoituksena oli selvittää, miten maantiellä tehtävässä työssä noudatetaan tienpitoviranomaisen antamia työ- ja liikenneturvallisuuteen liittyviä lupaehtoja. Työ tehtiin ELY-keskuksen liikenne- ja infrastruktuuri -vastuualueen kunnossa-pitoyksikössä, joka myöntää luvat maanteillä työskentelyyn. Lähtöaineisto opinnäytetyöhön kerätti...

  16. Yhteisöllisyys nuorten ehkäisevässä päihdetyössä

    OpenAIRE

    Helmiö, Susanna

    2010-01-01

    Yhteisöllisyys nuorten ehkäisevässä päihdetyössä opinnäytetyö toteutettiin Humanistisen ammattikorkeakoulun alaisen ehkäisevän päihdetyön osaamiskeskus Preventiimin hankkeen osakokonaisuutena. Hankkeessa pyrittiin selvittämään nuorten kokemuksia yhteisöllisyydestä ja ehkäisevästä päihdetyöstä. Opinnäytetyössä Espoon nuorisovaltuusto kertoi kokemuksiaan nuorten päihteiden käytöstä ja yhteisöllisyydestä. Nuorisovaltuustolaiset toimivat asiantuntijoina nuorten elämästä, ei oman toimintansa kerto...

  17. Dissociation between the effects of somatostatin (SS) and octapeptide SS-analogs on hormone release in a small subgroup of pituitary- and islet cell tumors

    NARCIS (Netherlands)

    L.J. Hofland (Leo); W.W. de Herder (Wouter); H.A. Visser-Wisselaar (Heleen); C. van Uffelen; M. Waaijers (Marlijn); J. Zuyderwijk; P. Uitterlinden (Piet); P.M. van Koetsveld (Peter); S.W.J. Lamberts (Steven); J.M. Kros (Johan)

    1997-01-01

    textabstractThe effects of somatostatin (SS-14 and/or SS-28) and of the three octapeptide SS-analogs that are available for clinical use (octreotide, BIM-23014 and RC-160) on hormone release by primary cultures of 15 clinically nonfunctioning pituitary adenomas (NFA), 7

  18. SS-sümbol stalinistide teenistuses / Uno Raudkivi

    Index Scriptorium Estoniae

    Raudkivi, Uno

    2005-01-01

    Eesti Waffen SS Diviisis teeninud autor leiab, et leegionäride süüdistamine fašismis ja natsionalismis on süütuse presumptsiooni rikkumine. Lisa: Uno Raudkivi sõjaväeteenistuse lühikronoloogia

  19. Outburst from SS 433 observed with BOOTES-2/COLORES

    Science.gov (United States)

    Caballero-Garcia, M. D.; Castro-Tirado, A.; Jelinek, M.; et al.

    2014-08-01

    In light of the recent extreme outburst of SS 433 (ATELs #6347, #6355) and follow-up by SWIFT (ATEL #6364) the 0.6m TELMA robotic telescope at the BOOTES-2 (+COLORES) astronomical station in Malaga (Spain), obtained optical 4000-9000 A spectra starting at 2014-08-01 and ending at 2014-08-07.

  20. Katariina kirikus algab Geeniuste mäss / Kai Ilustrumm

    Index Scriptorium Estoniae

    Ilustrumm, Kai

    2004-01-01

    Briti Nõukogu toetusel Pärnus, Tartus ja Tallinnas toimuvatel inglise filmide päevadel "Geeniuste mäss" näidatakse ka 3 Lindsay Andersoni nn. Travise triloogia filmi "Kui..." (1968), "Oo, õnneseen!" (1973) ja "Ravila Britannia" (1982), kus Mick Travise rollis on Malcolm McDowell (1943). Lisatud kava

  1. 姬松茸粗多糖对K562细胞增殖、凋亡的影响及其作用机制探讨%Explore of the effects of Agaricus blazei polysaccharide on proliferation and apoptosis of cells K562 and its mechanism

    Institute of Scientific and Technical Information of China (English)

    林祥伟; 张苏伟

    2015-01-01

    目的:研究姬松茸粗多糖在K562细胞的增殖、凋亡,以及对转录因子NF-κB的影响,并探究其中的作用机制。方法培养K562细胞,再分别将(2、5、10mg/mL)姬松茸粗多糖加入其中作用48h,之后应用流式细胞术、免疫荧光法等方法观察姬松茸粗多糖在K562细胞的增殖、凋亡和对NF-κB活性中的影响。结果姬松茸粗多糖对K562细胞增殖有很显著的抑制作用,还可以阻止细胞从G0/G1期到G2/M+S期过渡。经过10mg/mL的姬松茸粗多糖诱导48h后,K562细胞CML细胞的凋亡率65.41%,较对照组显著增加,差异有统计学意义(P<0.01)。而且NF-κB较对照组减少。结论姬松茸粗多糖能抑制K562细胞增殖,并且可以诱导其凋亡,其中的作用机制和NF-κB通道的异常激活明显相关,值得进一步的研究。%Objective To study the influence of Agaricus blazei on proliferation and apoptosis of cells K562 and transcription NF- κB, and to explore the mechanism.Methods Cells K562 were cultured, then the 2, 5,10mg/mL of Agaricus blazei polysaccharides were respectively added to react for 48 hours, and then the effects of Agaricus blazei on proliferation and apoptosis of cells K562 and activity of NF- κB were observed with the methods such as flow cytometry, immunofluorescence.Results Agaricus blazei significantly inhibited the proliferation of cells K562, and also could prevent the cell transition from phase G0/G1 to phase G2/M+S. After induction by 10mg/mL of Agaricus blazei for 48 hours, apoptosis rate of cells K562 was 65.41%, which was significantly higher than that in control groups, and the differences were statistically significant (P<0.01). However there were fewer factors of NF-κB than the control groups.Conclusion Agaricus blazei can inhibit the proliferation of cells K562, and can induce apoptosis of them, the mechanism of that is obviously related with abnormal activation of channel of factors NF- κ

  2. Immunomodulatory Effects of the Agaricus blazei Murrill-Based Mushroom Extract AndoSan in Patients with Multiple Myeloma Undergoing High Dose Chemotherapy and Autologous Stem Cell Transplantation: A Randomized, Double Blinded Clinical Study

    Directory of Open Access Journals (Sweden)

    Jon-Magnus Tangen

    2015-01-01

    Full Text Available Forty patients with multiple myeloma scheduled to undergo high dose chemotherapy with autologous stem cell support were randomized in a double blinded fashion to receive adjuvant treatment with the mushroom extract AndoSan, containing 82% of Agaricus blazei Murrill (19 patients or placebo (21 patients. Intake of the study product started on the day of stem cell mobilizing chemotherapy and continued until the end of aplasia after high dose chemotherapy, a period of about seven weeks. Thirty-three patients were evaluable for all study endpoints, while all 40 included patients were evaluable for survival endpoints. In the leukapheresis product harvested after stem cell mobilisation, increased percentages of Treg cells and plasmacytoid dendritic cells were found in patients receiving AndoSan. Also, in this group, a significant increase of serum levels of IL-1ra, IL-5, and IL-7 at the end of treatment was found. Whole genome microarray showed increased expression of immunoglobulin genes, Killer Immunoglobulin Receptor (KIR genes, and HLA genes in the Agaricus group. Furthermore, AndoSan displayed a concentration dependent antiproliferative effect on mouse myeloma cells in vitro. There were no statistically significant differences in treatment response, overall survival, and time to new treatment. The study was registered with Clinicaltrials.gov NCT00970021.

  3. Experimental atomic scale investigation of irradiation effects in CW 316SS and UFG-CW 316SS

    Science.gov (United States)

    Pareige, P.; Etienne, A.; Radiguet, B.

    2009-06-01

    Materials of the core internals of pressurized water reactor (austenitic stainless steels) are subject to neutron irradiation. To understand the ageing mechanisms associated with irradiation and propose life predictions of components or develop new materials, irradiation damage needs to be experimentally investigated. Atomic scale investigation of a neutron-irradiated CW316 SS with the laser pulsed atom probe gives a detailed description of the solute segregation in the austenitic grains. In order to understand the mechanism of solute segregation detected in the neutron-irradiated materials, ion irradiations were performed. These latest irradiations were realized on a CW 316SS as well as on a nanostructured CW 316SS. The study of irradiation effects in a nanograin material allows first, to easily analyse grain boundary segregation and second, to test the behaviour under irradiation of a new nanostructured material. The three aspects of this atomic scale investigation (neutron irradiation effect, model ion irradiation, new nanostructured CW 316 SS) are tackled in this paper.

  4. Imaging Transition Zone Thickness Beneath South America from SS Precursors

    Science.gov (United States)

    Schmerr, N.; Garnero, E.

    2006-12-01

    We image detailed upper mantle discontinuity structure beneath a number of geologically active regions, including the South American subduction zone, the Scotia plate subduction zone, and several volcanic hotspots (e.g., the Galapagos Islands), in a region ~10,000 km by 10,000 km wide, spanning 70° S to 20° N and 20° W to 110° W. Precursors to the seismic phase SS are analyzed, which form as a result of underside reflections off seismic discontinuities beneath the midpoint of the SS path and are highly sensitive to discontinuity depth and sharpness. Our SS dataset consists of over 15,000 high-quality transverse component broadband displacement seismograms collected from the Incorporated Research Institutions for Seismology (IRIS), the Canadian National Seismic Network (CNSN), as well as data from EarthScope seismic stations, and from the Canadian Northwest Experiment (CANOE) temporary broadband array deployment. This dataset densely samples several regions in our study area and significantly improves the sampling for this area compared to previous precursor studies. Data with common central SS bouncepoints are stacked to enhance precursory phases. Solution discontinuity structure depends on a number of factors, including dominant seismic period, crustal correction, signal-to-noise ratio threshold, and tomography model used for mantle heterogeneity correction. We exclude precursor data predicted to interfere with other seismic phases, such as topside reflections (e.g., s670sS), which have been demonstrated to contaminate final stacks. Solution transition zone thickness is at least 20 km thicker than global average estimates of 242 km along the northwestern portion of the South American subduction complex (Peru, Ecuador, and Columbia); this thickening extends 1000-1500 km to the east beneath the continent, but does not appear to continue south of -20° latitude along the convergent margin. A minimum of 10 km of thickening is imaged to the west of the Scotia

  5. Optical results of the black hole microquasar SS 433

    Institute of Scientific and Technical Information of China (English)

    2010-01-01

    We present optical spectroscopic observations of SS 433 during different precessional and orbital phases.Our 2004,2007 and 2008 data provide us a good chance to study the variability of the Hα line at different orbital phases for nearly the same disk inclination.The data show that the Hα of SS 433 usually had a symmetric profile between orbital phases 0.25-0.75,while an asymmetric structure with a strong red peak was observed for other orbital phases.We suggest that the orbital variability of Hα emission is connected with accretion flow from the donor star to the accretion disk.In addition,we attribute the dramatic increase of Hα emission during our 2007 observational run,which had a time scale of one day,to the emergence of strong jets.

  6. Vuorotyössä jaksaminen kliinisessä laboratoriossa

    OpenAIRE

    Kurtti, Elina; Niemi, Saija

    2014-01-01

    Opinnäytetyön tarkoituksena oli tutkia Vaasan keskussairaalan kliinisen laboratorion henkilökunnan vuorotyössä jaksamista. Opinnäytetyön tavoitteena oli selvittää kliiniselle laboratoriolle sen työntekijöiden jaksamista ja sitä, ovatko työntekijät tyytyväisiä työaikoihinsa ja olisiko niissä parannettavaa. Aihe saatiin Vaasan keskussairaalasta. Opinnäytetyössä tutkittiin, kuinka henkilökunta jaksaa vuorotyötä ja millaisia keinoja henkilökunta käyttää, jotta jaksaisi paremmin vuorotyötä. Opinnä...

  7. LoCuSS: Testing hydrostatic equilibrium in galaxy clusters

    Science.gov (United States)

    Smith, G. P.; Mazzotta, P.; Okabe, N.; Ziparo, F.; Mulroy, S. L.; Babul, A.; Finoguenov, A.; McCarthy, I. G.; Lieu, M.; Bahé, Y. M.; Bourdin, H.; Evrard, A. E.; Futamase, T.; Haines, C. P.; Jauzac, M.; Marrone, D. P.; Martino, R.; May, P. E.; Taylor, J. E.; Umetsu, K.

    2016-02-01

    We test the assumption of hydrostatic equilibrium in an X-ray luminosity selected sample of 50 galaxy clusters at 0.15 sub-4 per cent, and our hydrostatic measurements of the same achieve excellent agreement between XMM-Newton and Chandra. The mean ratio of X-ray to lensing mass for these 50 clusters is β_X= 0.95± 0.05, and for the 44 clusters also detected by Planck, the mean ratio of Planck mass estimate to LoCuSS lensing mass is β_P= 0.95± 0.04. Based on a careful like-for-like analysis, we find that LoCuSS, the Canadian Cluster Comparison Project, and Weighing the Giants agree on β_P ≃ 0.9-0.95 at 0.15 Planck cosmology results from the cosmic microwave background and galaxy cluster counts.

  8. Varhaisen tuen toimintamalli Ahlsell Oy:ssä

    OpenAIRE

    Vihtonen, Anne-Mari

    2012-01-01

    Varhaisen tuen toimintamalli Ahlsell Oy:ssä Vuosi 2012 Sivumäärä 51 Opinnäytetyöni tarkoituksena on selvittää mitä on hyvä johtaminen ja miten se vaikuttaa työ-hyvinvointiin. Työhyvinvointi on paljon puhuttu asia eikä suotta, koska siihen pitää kiinnittää erityistä huomiota, jotta henkilöstö jaksaisi työssään aina eläkeikään asti. Opinnäytetyöni tavoitteena on luoda toimintamalli, jota esimiehet ja johtajat voivat käyttää toimintansa tuk...

  9. Fluence dependence of deuterium retention in oxidized SS-316

    Science.gov (United States)

    Oya, Yasuhisa; Suzuki, Sachiko; Matsuyama, Masao; Hayashi, Takumi; Yamanishi, Toshihiko; Asakura, Yamato; Okuno, Kenji

    2011-10-01

    The ion fluence dependence of deuterium retention in SS-316 during oxidation at a temperature of 673 K was studied to evaluate the dynamics of deuterium retention in the oxide layer of SS-316. The correlation between the chemical state of stainless steel and deuterium retention was evaluated using XPS and TDS. It was found that the major deuterium desorption temperatures were located at around 660 K and 935 K, which correspond to the desorption of deuterium trapped as hydroxide. The deuterium retention increased with increasing deuterium ion fluence, since the deuterium retention as hydroxide increased significantly. However, retention saturated at an ion fluence of ˜2.5 × 10 21 D + m -2. The XPS result showed that FeOOD was formed on the surface, although pure Fe also remained in the oxide layer. These facts indicate the nature of the oxide layer have a key role in deuterium trapping behavior.

  10. Kumin kierrätys Teknikum Oy:ssä

    OpenAIRE

    Aaltonen, Katri

    2012-01-01

    Tämän työn tarkoituksena oli selvittää eri kierrätysmahdollisuuksia Teknikum Oy:ssä syntyvälle kumijätteelle. Alun perin työn tarkoituksena oli selvittää, onko yrityksen jätekumia mahdollista käyttää Valkeakoskella sijaitsevan Peatec Oy:n pyrolyysireaktorin raaka-aineena. Työn edetessä otettiin tarkastelun alle myös muita yrityksessä käynnissä olevia projekteja perustuen kierrätyskumin käyttöön raaka-aineena. Työssä esitellään Kuusakoski Oy:n valmistama kumipuru materiaalina, sekä uuden yhtei...

  11. Microstructure characteristic of SS304/BNi-2/SS304 butt joint by high temperature brazing%SS304/BNi-2/SS304高温钎焊对接接头微观结构特征

    Institute of Scientific and Technical Information of China (English)

    竺国荣; 陈虎; 祝金丹; 巩建鸣

    2011-01-01

    通过试验手段研究了SS304/BNi-2/SS304钎焊对接接头微观结构特征,使用能谱分析结合合金三元相图研究了接头区域典型的微观结构形貌和特征相变化规律,讨论了降熔元素在接头等温凝固连接过程中所起的关键作用。结果表明,在钎焊温度时,B从液相钎料向固相母材的快速扩散控制了接头的等温凝固过程,也因此决定了接头的微观结构相特征;钎焊接头通常会存在4种典型的相特征区域;钎角质量对于整个钎焊接头性能有重要影响。%The microstructure characteristic of SS304/Bni -2/SS304 butt joint was analyzed by high temperature brazing through the experimental research. Some typical microstructural morphologies and phase transition feature of the butt joint were studied by using ternary phase diagrams and EDS analysis. The critical effect of element diffusion on isothermal solidification of brazed joint was also discussed by analyzing the microstructural evolution. The results showed that the quick diffusing of boron from the liquid filler alloy into solid base metal controlled the isothermal solidification process as well as the joint microstructure. Four typical characteristic zones could be observed for the whole brazed joint. The fillet quality played a very important role in the whole joint performance.

  12. Ryhmässä toimiminen ja sosiaaliset taidot joukkueurheilussa

    OpenAIRE

    Liekola, Harri; Siikonen, Jyrki

    2015-01-01

    TIIVISTELMÄ KYMENLAAKSON AMMATTIKORKEAKOULU Sosiaalialan koulutusohjelma LIEKOLA, HARRI SIIKONEN, JYRKI Ryhmässä toimiminen ja sosiaaliset taidot joukkueurheilussa Opinnäytetyö 46 sivua + 9 liitesivua Työn ohjaaja Tuija Suikkanen-Malin Toimeksiantaja Kouvolan Pallonlyöjät ry. Pohjois-Kymen liikuntayhdistys ry. Toukokuu 2015 Avainsanat: ryhmätoiminta, sosiaaliset taidot, junioriurheilu, joukkueurheilu, pesäpallo, jääkiekko Opinnäytetyön tar...

  13. Revisiting the HST Fine Guidance Sensor Parallax of SS Cygni

    Science.gov (United States)

    Harrison, Thomas E.; McArthur, Barbara

    2016-01-01

    Recently, our results for the HST FGS parallax of SS Cyg (Harrison et al. 2000) have been called into question due to a recent radio parallax for this object (Miller-Jones et al. 2013). We have re-analyzed the data for our FGS program on SS Cyg and derive a revised parallax of 7.3 +/- 0.2 mas (137 pc). This parallax is 17% larger than that originally published. We discuss the new analysis effort, and the reasons for the change in our result. We believe we have identified why the radio parallax (8.77 mas) disagrees with the FGS results: We find that if we allow for a roll of two degrees in the HST coordinate system, we obtain the discrepant VLBA radio parallax. It is impossible, however, for the error in the roll of HST to be more than a few tenths of a degree, or else the FGS would not lock-on to the program targets. This roll leads to the large, and erroneous VLBA parallax for SS Cyg. We note that the radio parallax for the black hole system V404 Cyg (Miller-Jones et al. 2009) is also much larger than the spectroscopic parallax of its secondary star. In addition, we closely examine the attempt by Nelan & Bond (2013) to reconcile the FGS data set with the radio result. We identify what we believe to be significant issues with their analysis. Finally, we present a quantitative abundance analysis for SS Cyg, RU Peg, and GK Per derived from moderate resolution K-band spectroscopy. We find severe carbon deficiencies (10 to 20% of the solar value) in the secondary stars of all three objects. We describe the Python program "kmoog" which generates synthetic spectra, and autonomously compares them to observational data.

  14. Self-adaptive OmpSs tasks in heterogeneous environments

    OpenAIRE

    Planas J.; Badia R.M.; Ayguade E.; Labarta J.

    2013-01-01

    As new heterogeneous systems and hardware accelerators appear, high performance computers can reach a higher level of computational power. Nevertheless, this does not come for free: the more heterogeneity the system presents, the more complex becomes the programming task in terms of resource management. OmpSs is a task-based programming model and framework focused on the runtime exploitation of parallelism from annotated sequential applications. This paper presents a set of extensions to this...

  15. Atle Næss: Roten av minus en

    Directory of Open Access Journals (Sweden)

    Linda Hamrin Nesby

    2006-06-01

    Full Text Available Atle Næss har skrevet flere romaner, og to biografier om hhv Edevard Munch og Galileo Galilei. Med Roten av minus en kombinerer han roman- og biografigenren, og skriver både en kjærlighetshistorie, et utkast til en biografi og et riss av en selvbiografi som i sum blir til en roman om hvorvidt livet har den orden og nødvendighet som biografien er satt til å formidle

  16. Elastic-plastic analysis of the SS-3 tensile specimen

    Energy Technology Data Exchange (ETDEWEB)

    Majumdar, S. [Argonne National Lab., IL (United States)

    1998-09-01

    Tensile tests of most irradiated specimens of vanadium alloys are conducted using the miniature SS-3 specimen which is not ASTM approved. Detailed elastic-plastic finite element analysis of the specimen was conducted to show that, as long as the ultimate to yield strength ratio is less than or equal to 1.25 (which is satisfied by many irradiated materials), the stress-plastic strain curve obtained by using such a specimen is representative of the true material behavior.

  17. LoCuSS: Testing hydrostatic equilibrium in galaxy clusters

    CERN Document Server

    Smith, G P; Okabe, N; Ziparo, F; Mulroy, S L; Babul, A; Finoguenov, A; McCarthy, I G; Lieu, M; Bahe, Y; Bourdin, H; Evrard, A E; Futamase, T; Haines, C P; Jauzac, M; Marrone, D P; Martino, R; May, P E; Taylor, J E; Umetsu, K

    2015-01-01

    We test the assumption of hydrostatic equilibrium in an X-ray luminosity selected sample of 50 galaxy clusters at $0.15SS). Our weak-lensing measurements of $M_{500}$ control systematic biases to sub-4 per cent, and our hydrostatic measurements of the same achieve excellent agreement between XMM-Newton and Chandra. The mean ratio of X-ray to lensing mass for these 50 clusters is $\\beta_{\\rm X}=0.95\\pm0.05$, and for the 44 clusters also detected by Planck, the mean ratio of Planck mass estimate to LoCuSS lensing mass is $\\beta_{\\rm P}=0.95\\pm0.04$. Based on a careful like-for-like analysis, we find that LoCuSS, the Canadian Cluster Comparison Project (CCCP), and Weighing the Giants (WtG) agree on $\\beta_{\\rm P}\\simeq0.9-0.95$ at $0.15

  18. Tritium uptake by SS316 and its decontamination

    Science.gov (United States)

    Torikai, Y.; Penzhorn, R.-D.; Matsuyama, M.; Watanabe, K.

    2004-08-01

    As-received and highly polished SS316 specimens were loaded with HT at 473-573 K. The uptake by polished samples was found to be up to five times that of as-received ones, when loading was performed immediately after polishing. This disparity vanished when polished specimens were subjected to a prolonged exposure to air prior to loading. The tritium loss from tritium-loaded SS316 specimens was examined by chemical etching and by thermal release in a flow system using various carrier gases at several temperatures. While at moderate temperatures the type of carrier has an impact on the tritium release rate, at higher ones this effect disappears. Moisture in the carrier gas has little influence on the loss rate of bulk tritium. Etching depth profiles of specimens previously heat-treated in the presence of air or Ar + H 2 and of untreated specimens are given. Evidence for chronic tritium liberation from SS316 at 298 K is provided.

  19. Investigation on SS316, SS440C, and Titanium Alloy Grade-5 used as Single Point Cutting Tool

    Directory of Open Access Journals (Sweden)

    Mr.Kothakota Suresh Kumar

    2015-01-01

    Full Text Available The main objective of this work is to find alternative materials for the cutting tools used in turning operations. The conventional materials like tungsten carbide(WC, titanium carbide(TiC, cubic boron nitride (CBN and diamond used as cutting tools for turning operations on lathe are expensive. Titanium grade 5 (Ti-6Al-4V, SS440C/AISI440C and SS316 are some of the materials which satisfy the necessary requirements for turning metals and polymer materials. These materials are machined as per the standard tool signature of high-speed steel tool (HSS and are subjected to necessary heat treatment for hardening and then finish ground. The machined tools thus prepared were used to turn mild steel and aluminium workpieces. The cutting forces at play are determined using lathe tool dynamometer and plotted on a MCD (Merchant’s Circle Diagram. The cutting tools are also subjected to tests to determine tool life, wear and work hardening. It is found that the performance and tool life of SS440C is better and cost effective compared to existing tools. Even though Ti-6Al-4V is comparatively costly it could be used for obtaining good surface finish.

  20. Research on Protection Measures of SS7%SS7信令安全防护措施研究

    Institute of Scientific and Technical Information of China (English)

    邵延峰

    2016-01-01

    SS7协议是PSTN网络的核心控制协议.随着网络融合和业务互通以及各种新技术、新业务的出现,PSTN网络的安全受到越来越多的关注.在对SS7协议架构和信号单元结构简要介绍的基础上,从信令协议设计和恶意用户攻击2个方面对PSTN网络可能面临的安全威胁进行了认真分析,提出了协议合法性检测、访问控制和FLOOD攻击防御等安全防护措施以提高SS7信令及其网络的安全性,并给出了具体操作字段和分析的内容.

  1. 基于OPNET的SS7协议MTP2层仿真实现%Simulation Realization of MTP2 in SS7 Based on OPNET

    Institute of Scientific and Technical Information of China (English)

    黄显安; 王可人

    2008-01-01

    SS7是通信网的神经系统,对通信网络的运行发挥着重要的核心作用.介绍了SS7协议的模型,分析了SS7的第2级--MTP2的功能和过程.详细阐述了如何利用OPNET实现MTP2层功能的过程.

  2. SS7电力机车再生制动的运用分析%Application of Regenerative Braking for SS1 Electric Locomotive

    Institute of Scientific and Technical Information of China (English)

    吴榕

    2008-01-01

    介绍了国产SS7型电力机车再生制动的工作特点,对SS7机车再生制动的使用进行了评价,并就SS7型电力机车再生制动运用存存的问题作了分析并提出改进建议.

  3. 20 CFR 225.24 - SS Earnings PIA used in survivor annuities.

    Science.gov (United States)

    2010-04-01

    ... 20 Employees' Benefits 1 2010-04-01 2010-04-01 false SS Earnings PIA used in survivor annuities... Amount of the Residual Lump-Sum Payable § 225.24 SS Earnings PIA used in survivor annuities. The Social Security Earnings PIA (SS Earnings PIA) used in survivor annuities may be used in computing the tier...

  4. 27 CFR 40.361 - Execution and filing of Form SS-4.

    Science.gov (United States)

    2010-04-01

    ... such district as provided for in 26 CFR § 301.6091-1. The application shall be signed by: (a) The... Form SS-4. 40.361 Section 40.361 Alcohol, Tobacco Products and Firearms ALCOHOL AND TOBACCO TAX AND... Execution and filing of Form SS-4. The application on IRS form SS-4, together with any...

  5. 32 CFR Appendix E to Part 246 - Stars and Stripes (S&S) Board of Directors

    Science.gov (United States)

    2010-07-01

    ... 32 National Defense 2 2010-07-01 2010-07-01 false Stars and Stripes (S&S) Board of Directors E... DEFENSE (CONTINUED) MISCELLANEOUS STARS AND STRIPES (S&S) NEWSPAPER AND BUSINESS OPERATIONS Pt. 246, App. E Appendix E to Part 246—Stars and Stripes (S&S) Board of Directors A. Organization and...

  6. Polysaccharide Agaricus blazei Murill stimulates myeloid derived suppressor cell differentiation from M2 to M1 type, which mediates inhibition of tumour immune-evasion via the Toll-like receptor 2 pathway.

    Science.gov (United States)

    Liu, Yi; Zhang, Lingyun; Zhu, Xiangxiang; Wang, Yuehua; Liu, WenWei; Gong, Wei

    2015-11-01

    Gr-1(+) CD11b(+) myeloid-derived suppressor cells (MDSCs) accumulate in tumor-bearing animals and play a critical negative role during tumor immunotherapy. Strategies for inhibition of MDSCs are expected to improve cancer immunotherapy. Polysaccharide Agaricus blazei Murill (pAbM) has been found to have anti-cancer activity, but the underlying mechanism of this is poorly understood. Here, pAbM directly activated the purified MDSCs through inducing the expression of interleukin-6 (IL-6), IL-12, tumour necrosis factor and inducible nitric oxide synthase (iNOS), CD86, MHC II, and pSTAT1 of it, and only affected natural killer and T cells in the presence of Gr-1(+) CD11b(+) monocytic MDSCs. On further analysis, we demonstrated that pAbM could selectively block the Toll-like receptor 2 (TLR2) signal of Gr-1(+) CD11b(+) MDSCs and increased their M1-type macrophage characteristics, such as producing IL-12, lowering expression of Arginase 1 and increasing expression of iNOS. Extensive study showed that Gr-1(+) CD11b(+) MDSCs by pAbM treatment had less ability to convert the CD4(+) CD25(-) cells into CD4(+) CD25(+) phenotype. Moreover, result from selective depletion of specific cell populations in xenograft mice model suggested that the anti-tumour effect of pAbM was dependent on Gr-1(+ ) CD11b(+) monocytes, nether CD8(+) T cells nor CD4(+) T cells. In addition to, pAbM did not inhibit tumour growth in TLR2(-/-) mice. All together, these results suggested that pAbM, a natural product commonly used for cancer treatment, was a specific TLR2 agonist and had potent anti-tumour effects through the opposite of the suppressive function of Gr-1(+) CD11b(+) MDSCs. PMID:26194418

  7. Polysaccharide Agaricus blazei Murill stimulates myeloid derived suppressor cell differentiation from M2 to M1 type, which mediates inhibition of tumour immune-evasion via the Toll-like receptor 2 pathway.

    Science.gov (United States)

    Liu, Yi; Zhang, Lingyun; Zhu, Xiangxiang; Wang, Yuehua; Liu, WenWei; Gong, Wei

    2015-11-01

    Gr-1(+) CD11b(+) myeloid-derived suppressor cells (MDSCs) accumulate in tumor-bearing animals and play a critical negative role during tumor immunotherapy. Strategies for inhibition of MDSCs are expected to improve cancer immunotherapy. Polysaccharide Agaricus blazei Murill (pAbM) has been found to have anti-cancer activity, but the underlying mechanism of this is poorly understood. Here, pAbM directly activated the purified MDSCs through inducing the expression of interleukin-6 (IL-6), IL-12, tumour necrosis factor and inducible nitric oxide synthase (iNOS), CD86, MHC II, and pSTAT1 of it, and only affected natural killer and T cells in the presence of Gr-1(+) CD11b(+) monocytic MDSCs. On further analysis, we demonstrated that pAbM could selectively block the Toll-like receptor 2 (TLR2) signal of Gr-1(+) CD11b(+) MDSCs and increased their M1-type macrophage characteristics, such as producing IL-12, lowering expression of Arginase 1 and increasing expression of iNOS. Extensive study showed that Gr-1(+) CD11b(+) MDSCs by pAbM treatment had less ability to convert the CD4(+) CD25(-) cells into CD4(+) CD25(+) phenotype. Moreover, result from selective depletion of specific cell populations in xenograft mice model suggested that the anti-tumour effect of pAbM was dependent on Gr-1(+ ) CD11b(+) monocytes, nether CD8(+) T cells nor CD4(+) T cells. In addition to, pAbM did not inhibit tumour growth in TLR2(-/-) mice. All together, these results suggested that pAbM, a natural product commonly used for cancer treatment, was a specific TLR2 agonist and had potent anti-tumour effects through the opposite of the suppressive function of Gr-1(+) CD11b(+) MDSCs.

  8. Medicinal Mushroom Growth as Affected by Non-Axenic Casing Soil

    Institute of Scientific and Technical Information of China (English)

    D. C. ZIED; M. T. A. MINHONI; J. KOPYTOWSKI-FILHO; L. BARBOSA; M. C. N. ANDRADE

    2011-01-01

    Ten different casing soils were collected from two soils at two depths (0.2 and 2.0 m below soil surface) to examine the relationships between the physical properties of non-axenic casing soil and yield, number and weight of the medicinal mushroom Agaricus blazei ss. Heinemann. The results showed that soil clay content and bulk density were negatively correlated with the mushroom yield,respectively, but soil silt content and water-holding capacity were found to be positively correlated with the yield. The number of mushrooms was negatively correlated with soil water-holding capacity but positively correlated with soil clay, bulk density and porosity.The weight of mushroom was positively correlated with the content of soil fine sand and negatively correlated with the contents of soil coarse sand, total sand and clay. Neither soil depth nor different soil combinations affected the yield and number of mushrooms, but the mushroom weight was affected by the soil combinations and soil depth, so interplay in the fructification process with the physical characteristics of casing is complicated.

  9. VirE2: a unique ssDNA-compacting molecular machine.

    Science.gov (United States)

    Grange, Wilfried; Duckely, Myriam; Husale, Sudhir; Jacob, Susan; Engel, Andreas; Hegner, Martin

    2008-02-01

    The translocation of single-stranded DNA (ssDNA) across membranes of two cells is a fundamental biological process occurring in both bacterial conjugation and Agrobacterium pathogenesis. Whereas bacterial conjugation spreads antibiotic resistance, Agrobacterium facilitates efficient interkingdom transfer of ssDNA from its cytoplasm to the host plant cell nucleus. These processes rely on the Type IV secretion system (T4SS), an active multiprotein channel spanning the bacterial inner and outer membranes. T4SSs export specific proteins, among them relaxases, which covalently bind to the 5' end of the translocated ssDNA and mediate ssDNA export. In Agrobacterium tumefaciens, another exported protein-VirE2-enhances ssDNA transfer efficiency 2000-fold. VirE2 binds cooperatively to the transferred ssDNA (T-DNA) and forms a compact helical structure, mediating T-DNA import into the host cell nucleus. We demonstrated-using single-molecule techniques-that by cooperatively binding to ssDNA, VirE2 proteins act as a powerful molecular machine. VirE2 actively pulls ssDNA and is capable of working against 50-pN loads without the need for external energy sources. Combining biochemical and cell biology data, we suggest that, in vivo, VirE2 binding to ssDNA allows an efficient import and pulling of ssDNA into the host. These findings provide a new insight into the ssDNA translocation mechanism from the recipient cell perspective. Efficient translocation only relies on the presence of ssDNA binding proteins in the recipient cell that compacts ssDNA upon binding. This facilitated transfer could hence be a more general ssDNA import mechanism also occurring in bacterial conjugation and DNA uptake processes. PMID:18303950

  10. VirE2: a unique ssDNA-compacting molecular machine.

    Directory of Open Access Journals (Sweden)

    Wilfried Grange

    2008-02-01

    Full Text Available The translocation of single-stranded DNA (ssDNA across membranes of two cells is a fundamental biological process occurring in both bacterial conjugation and Agrobacterium pathogenesis. Whereas bacterial conjugation spreads antibiotic resistance, Agrobacterium facilitates efficient interkingdom transfer of ssDNA from its cytoplasm to the host plant cell nucleus. These processes rely on the Type IV secretion system (T4SS, an active multiprotein channel spanning the bacterial inner and outer membranes. T4SSs export specific proteins, among them relaxases, which covalently bind to the 5' end of the translocated ssDNA and mediate ssDNA export. In Agrobacterium tumefaciens, another exported protein-VirE2-enhances ssDNA transfer efficiency 2000-fold. VirE2 binds cooperatively to the transferred ssDNA (T-DNA and forms a compact helical structure, mediating T-DNA import into the host cell nucleus. We demonstrated-using single-molecule techniques-that by cooperatively binding to ssDNA, VirE2 proteins act as a powerful molecular machine. VirE2 actively pulls ssDNA and is capable of working against 50-pN loads without the need for external energy sources. Combining biochemical and cell biology data, we suggest that, in vivo, VirE2 binding to ssDNA allows an efficient import and pulling of ssDNA into the host. These findings provide a new insight into the ssDNA translocation mechanism from the recipient cell perspective. Efficient translocation only relies on the presence of ssDNA binding proteins in the recipient cell that compacts ssDNA upon binding. This facilitated transfer could hence be a more general ssDNA import mechanism also occurring in bacterial conjugation and DNA uptake processes.

  11. Wapice IoT-Ticket.com -valvomoalusta kiinteistöautomaatiokäytössä

    OpenAIRE

    Vehmaanperä, Joonas

    2016-01-01

    ssä opinnäytetyössä tutkittiin Wapice Oy:n IoT-Ticket.com -valvomoalustan soveltuvuutta kiinteistöautomaation valvontaan ja Enermix Oy:n tarpeisiin. Työssä käsiteltiin IoT-Ticket.com -alustan ominaisuuksia ja toimintoja vertailemalla niitä nykyisin Enermix Oy:n käytössä olevaan valvomoalustaan. Vertailutasona käytettiin nykyisellä valvomoalustalla toteutettua poistoilman lämmöntalteenotto kohteen valvomoa. Opinnäytetyössä tärkeimpinä vertailtavia seikkoja valvomoalustojen välillä olivat työ...

  12. QR-koodi turvallisuusviestinnässä

    OpenAIRE

    Käkönen, Valtteri

    2016-01-01

    Tämän päättötyön aiheena oli kartoittaa QR-koodin käyttöä ja käyttömahdollisuuksia turvallisuusviestinnässä. QR-koodi on lyhenne sanoista Quick Response ja tämä tekniikka on lähtöisin tehtaista, joissa liukuhihnalla kulkevien tavaroiden nopea tunnistaminen on tarpeellista. QR-koodin voi lukea kamerallisella mobiililaitteella. Yleensä QR-koodi luodaan internetsivusta, jolloin käyttäjä on helppo ohjata halutun aineiston ääreen URL-osoitteen pituudesta riippumatta. Selvitys aloitettiin etsim...

  13. Verkon laitteiden valvonta teollisuusympäristössä

    OpenAIRE

    Taskila, Antti

    2012-01-01

    Tämä on Ruukki Oy:n Raahen tehtaalle toteutettu opinnäytetyö, jonka tavoite oli luoda ohjelma ja valvoa siihen lisättyjen laitteiden saavutettavuutta verkossa. Aiemmin tiedot toimimattomista laitteista kulkivat useamman välikäden kautta esimerkiksi työntekijältä työnjohtajalle, joka taas ottaa yhteyttä parhaakseen katsomaansa henkilöön. Tässä ketjussa saattaa kestää pitkiäkin aikoja. Tästä johtuva tauko saattaa laskea tuotannon laitteiden toimintatehoa suurestikin. Aluksi mietittiin eri t...

  14. Mielenterveyskuntoutujien ruokailutottumusten ‎kartoitus EMY ry:ssä

    OpenAIRE

    Luukkonen, Anni; Isokangas, Anna-Liisa

    2010-01-01

    Opinnäytetyömme tarkoituksena on kartoittaa mielenterveyskuntoutujien näkemyksiä terveellisestä ‎ruoasta, heidän omia ruokailutottumuksiaan ja näkemyksiä talkooruokailusta Espoon ‎mielenterveysyhdistys ry:ssä (EMY ry). EMY ry tekee töitä mielenterveyden edistämiseksi Espoon ‎seudulla. EMY ry on esittänyt Laurea-ammattikorkeakoulun hankefoorumipäivillä tutkimusideansa ‎talkooruokailunsa kehittämisestä. Toteutimme opinnäytetyön osana Työn rajalla- hanketta, joka ‎on osa Laurea-ammattikorkeakoul...

  15. Interaktiiviset valkotaulut opetuskäytössä

    OpenAIRE

    Pääkkönen, Tero

    2010-01-01

    Tämän työn tarkoituksena oli kuvata interaktiivisten valkotaulujen käyttöä ja ominaisuuksia opetuskäytössä. Interaktiiviseksi valkotauluksi kutsutaan järjestelmää, jossa on tyypillisesti yhdistettynä tietokone, dataprojektori ja valkotaulu liikkeentunnistustekniikalla. Tällöin laitteisto asennetaan paikalleen kiinteästi. Interaktiivisen valkotaulun toimintaperiaatteena on heijastaa tietokoneen näyttö valkotaululle, joka toimii suurena kosketusnäyttönä. Valkotaulun käytetyimmät liikkeentunnist...

  16. Materiaalivirtojen tehostaminen Mac Steel Oy:ssä

    OpenAIRE

    Toivonen, Mika

    2011-01-01

    Työssä tutkitaan Mac Steel Oy:n materiaalivirta ongelmia, ongelman lähteitä ja keinoja päästä ongelmista eroon. Mac Steelin tuotanto on keskitytty suuriin kappaleisiin, jotka aiheuttavat tietynlaisia ongelmia materiaalien ja valmiiden tuotteiden käsittelyssä. Asiakaskunta on laaja johon kuuluu maamme johtavia alumiiniveneiden ja ajoneuvoteollisuuden yrityksiä, lisäksi tuotteita valmistetaan teräsrakenne teollisuuden tarpeisiin. Lähtökohtana on tutkia Lean ajatusmaailmaa ja sieltä löytää ne...

  17. Pilvipalvelut yrityskäytössä

    OpenAIRE

    Farsi Madan, Alex Mehdi

    2016-01-01

    Insinöörityössä perehdyttiin pilvipalveluihin ja siihen, miksi niiden suosio on noussut viime vuosien aikana. Tavoitteena oli selvittää, mitä toimenpiteitä kohdeyrityksen täytyy tehdä Google Apps for Workin käyttöönotossa. Google Apps for Work on Googlen yrityksille tarjoama palvelupaketti, joka sisältää web-pohjaisia viestintä- ja yhteistyösovelluksia. Opinnäytetyön tavoitteena oli vertailla puolueettomasti kahta yhteistyösovellusten markkinajohtajaa Googlea ja Microsoftia. Lisäksi tavo...

  18. Grafiikan ohjaus Vizrt-järjestelmässä

    OpenAIRE

    Makkonen, Jani

    2013-01-01

    Ensin työssä esitellään Vizrt-järjestelmän eri osat eli Content Pilot, Viz Artist, Viz Template Wizards ja Viz Engine. Content Pilot on sapluunoiden käyttämiseen tarkoitettu sovellus. Viz Artist taas on grafiikan tekemiseen tarkoitettu sovellus. Template Wizardilla tehdään sapluunoita ja Viz Enginessä toistetaan valmiit scenet eli näytetään grafiikka. Sitten käydään läpi mitä grafiikan ohjaaminen tarkoittaa, kerrotaan mitä scenet ja sapluunat ovat ja kerrotaan mitä ovat sapluunoiden v...

  19. Pukusuunnittelu näytelmässä Transfinlandia

    OpenAIRE

    Syrjäkari, Tellervo

    2014-01-01

    Lopputyöni taiteellinen osuus on pukusuunnittelu Paula Vesalan kirjoittamaan näytelmään Transfinlandia, jonka kantaesitys sai ensi-iltansa Mediakeskus Lumen studionäyttämöllä 10.4.2014. Näytelmän ohjasi Piia Peltola. Lopputyöni kirjallinen osuus nojaa kokonaisuudessaan taiteelliseen työskentelyyni kyseisessä näytelmässä. Näytelmä kertoo tarinan sukupuolenkorjausprosessia läpikäyvästä pojasta, josta tulee nainen. Olen rajannut kirjallisen työni käsittelemään taiteellista pukusuunnittelut...

  20. Osaaminen monipalvelutyössä ruoka- ja puhdistuspalvelualalla

    OpenAIRE

    Enqvist, Annika

    2015-01-01

    Opinnäytetyön tavoitteena oli selvittää ruoka- ja puhdistuspalvelualan monipalvelutyön osaamisvaatimuksia kunta-alalla. Lisäksi selvitettiin, miten työntekijä voi hankkia monipalvelutyössä tarvittavan osaamisen. Tutkimusote oli kvalitatiivinen, tutkimusmenetelmä teemahaastattelu ja sähköpostikysely. Haastatteluihin osallistui yksitoista ruoka- ja puhdistuspalvelualan asiantuntijaa, ja viisi asiantuntijaa vastasi sähköpostitse. Vastaukset jaoteltiin kolmeen osaamispääomaluokkaan: henkilö-,...

  1. Musiikki ja musiikkiterapia vaihtoehtoisena hoitomuotona hoitotyössä

    OpenAIRE

    Pelho, Veera

    2013-01-01

    Musiikilla on ihmiseen monimuotoisia vaikutuksia sekä fyysisesti että psyykkisesti. Sitä on käytetty universaalisesti jo vuosisatojen ajan apuna ihmiselämän muutoksissa. Tämä opinnäytetyö käsittelee musiikin ja musiikkiterapian käyttöä somaattisesti sairaiden potilaiden parissa. Opinnäytetyön tarkoitus on selvittää voiko musiikki ja musiikkiterapia olla osa hoitotyötä, voiko siitä olla apua hoitotyössä sekä tarkastella millaisia hoitotuloksia musiikilla ja musiikkiterapialla on saatu. ...

  2. KOTI KIIKARISSA : Kokemusasiantuntijuus maahanmuuttajien asumiskoulutustyössä

    OpenAIRE

    Haltia, Eero

    2012-01-01

    TIIVISTELMÄ Haltia, Eero. Koti Kiikarissa - Kokemusasiantuntijuus maahanmuuttajien asumiskoulutustyössä. Järvenpää, syksy 2012, 70s., 4 liitettä. Diakonia-ammattikorkeakoulu, Järvenpään yksikkö, Sosiaalialan koulutusohjelma, Diakonian suuntautumisvaihtoehto, sosionomi - diakoni (AMK) Kehittämishankkeen tarkoituksena on julkistaa kokemuksia maahanmuuttajan kokemusasiantuntijuuden käytöstä heidän asumiskoulutuksessaan. Toimintaympäristönä on Espoon kaupunki ja Espoon Järjestöjen Yhteis...

  3. Study on dynamic performance tests for SS7B and SS7C type electric locomotives%SS7B与SS7C型电力机车的动力学性能试验研究

    Institute of Scientific and Technical Information of China (English)

    俞展猷; 封全保

    2000-01-01

    通过对在同一条线路上,结构基本相同但轴重不同,悬挂参数也进行了优化的两台SS7型电力机车进行的动力学性能试验,研究了轴重和悬挂参数对机车动力学性能的影响,得到了有价值的结论,为机车的设计提供了重要的参考资料.

  4. Kotouttava ja ohjaava ote pakolaisperhetyössä

    OpenAIRE

    Toom, Nita; Vesterinen, Tanja

    2012-01-01

    Tiivistelmä Opinnäytetyön tarkoituksena oli tutkia Jyväskylän maahanmuuttajapalveluiden asiakkaana olevien pakolaisperheiden kanssa tehtävää lastensuojelullista perhetyötä. Toimeksiantajanamme toimi Jyväskylän kaupungin maahanmuuttajapalvelut. Tavoitteena oli selvittää millaista perhetyötä pakolaisperheiden kanssa tehdään, mikä tukee perhetyön onnistumista ja mitä erityispiirteitä ja haasteita perhetyön toteutumiseen liittyy. Pyrimme myös selvittämään, miten perhetyössä näkyy perheen kot...

  5. Hiljaisen tiedon mallittaminen Suominen Kuitukankaat Oy:ssä

    OpenAIRE

    Järvistö, Tero

    2007-01-01

    Tämän opinnäytetyön aiheena on hiljaisen tiedon mallittaminen Suominen Kuitukankaat Oy:ssä. Tutkimuksen tarkoituksena oli löytää keino yrityksen avainhenkilöiden hiljaisen tiedon siirtämiseen mahdollisimman eksplisiittiseen muotoon. Tavoitteena oli luoda yritykselle konkreettinen käytäntö, jonka avulla se voi pyrkiä siirtämään hiljaista tietoa dokumentoituun muotoon. Työn teoriaosassa esitetään hiljaisen tiedon määritelmä sekä käsitellään sen muuttamista eksplisiittiseen muotoon. Lisäksi pohd...

  6. Cardiac involvement in patients with systemic lupus erythematosus and correlation of valvular lesions with anti-Ro/SS-A and anti-La/SS-B antibody levels.

    Science.gov (United States)

    Shahin, Amira A; Shahin, Hesham A; Hamid, Magdy A; Amin, Mona A

    2004-01-01

    The aim of this study was to evaluate the incidence of morphologic and functional cardiac abnormalities in patients with systemic lupus erythematosus (SLE) and to correlate the findings with levels of anti-Ro/SS-A, anti-La/SS-B, and anti-cardiolipin antibody (aCL). Sixty-two patients with SLE were enrolled in this study. All patients underwent complete history taking, clinical assessment, and standard two-dimensional and Doppler echocardiography. Anti-Ro/SS-A, anti-La/SS-B, and aCL levels were measured using a standardized ELISA test. The patients were subdivided into two subgroups based on the presence or absence of valvular involvement. The two subgroups were then compared. Valvular involvement was present in 19 patients (30.6%), pericardial effusion in 12 patients (19.4%), impaired left ventricular relaxation abnormalities in 2 patients (3.2%), and pulmonary hypertension in 3 patients (4.8%). More patients in the valvular involvement group had positive anti-Ro/SS-A antibodies than in the valvular noninvolvement group (7/19 vs. 4/43). The difference was significant, with P anti-Ro/SS-A levels were significantly higher in the valvular involvement group (33.7 +/- 36.0 vs. 13.7 +/- 25.1; P anti-Ro/SS-A and anti-La/SS-B antibodies and the pathogenesis of the valvular lesions in SLE patients.

  7. Acousto-microfluidics for screening of ssDNA aptamer

    Science.gov (United States)

    Park, Jee-Woong; Lee, Su Jin; Ren, Shuo; Lee, Sangwook; Kim, Soyoun; Laurell, Thomas

    2016-01-01

    We demonstrate a new screening method for obtaining a prostate-specific antigen (PSA) binding aptamer based on an acoustofluidic separation (acoustophoreis) technique. Since acoustophoresis provides simultaneous washing and separation in a continuous flow mode, we efficiently obtained a PSA binding aptamer that shows high affinity without any additional washing step, which is necessary in other screening methods. In addition, next-generation sequencing (NGS) was applied to accelerate the identification of the screened ssDNA pool, improving the selecting process of the aptamer candidate based on the frequency ranking of the sequences. After the 8th round of the acoustophoretic systematic evolution of ligands by exponential enrichment (SELEX) and following sequence analysis with NGS, 7 PSA binding ssDNA aptamer-candidates were obtained and characterized with surface plasmon resonance (SPR) for affinity and specificity. As a result of the new SELEX method with PSA as the model target protein, the best PSA binding aptamer showed specific binding to PSA with a dissociation constant (Kd) of 0.7 nM. PMID:27272884

  8. Reversibly shielded DNA polyplexes based on bioreducible PDMAEMA-SS-PEG-SS-PDMAEMA triblock copolymers mediate markedly enhanced nonviral gene transfection.

    Science.gov (United States)

    Zhu, Caihong; Zheng, Meng; Meng, Fenghua; Mickler, Frauke Martina; Ruthardt, Nadia; Zhu, Xiulin; Zhong, Zhiyuan

    2012-03-12

    Reversibly shielded DNA polyplexes based on bioreducible poly(dimethylaminoethyl methacrylate)-SS-poly(ethylene glycol)-SS-poly(dimethylaminoethyl methacrylate) (PDMAEMA-SS-PEG-SS-PDMAEMA) triblock copolymers were designed, prepared and investigated for in vitro gene transfection. Two PDMAEMA-SS-PEG-SS-PDMAEMA copolymers with controlled compositions, 6.6-6-6.6 and 13-6-13 kDa, were obtained by reversible addition-fragmentation chain transfer (RAFT) polymerization of dimethylaminoethyl methacrylate (DMAEMA) using CPADN-SS-PEG-SS-CPADN (CPADN: 4-cyanopentanoic acid dithionaphthalenoate; PEG: 6 kDa) as a macro-RAFT agent. Like their nonreducible PDMAEMA-PEG-PDMAEMA analogues, PDMAEMA-SS-PEG-SS-PDMAEMA triblock copolymers could effectively condense DNA into small particles with average diameters less than 120 nm and close to neutral zeta potentials (0 ∼ +6 mV) at and above an N/P ratio of 3/1. The resulting polyplexes showed excellent colloidal stability against 150 mM NaCl, which contrasts with polyplexes of 20 kDa PDMAEMA homopolymer. In the presence of 10 mM dithiothreitol (DTT), however, polyplexes of PDMAEMA-SS-PEG-SS-PDMAEMA were rapidly deshielded and unpacked, as revealed by significant increase of positive surface charges as well as increase of particle sizes to over 1000 nm. Release of DNA in response to 10 mM DTT was further confirmed by gel retardation assays. These polyplexes, either stably or reversibly shielded, revealed a low cytotoxicity (over 80% cell viability) at and below an N/P ratio of 12/1. Notably, in vitro transfection studies showed that reversibly shielded polyplexes afforded up to 28 times higher transfection efficacy as compared to stably shielded control under otherwise the same conditions. Confocal laser scanning microscope (CLSM) studies revealed that reversibly shielded polyplexes efficiently delivered and released pDNA into the perinuclei region as well as nuclei of COS-7 cells. Hence, reduction-sensitive reversibly shielded DNA

  9. Johtamisen työkalut vapaaehtoistyössä : Viestintä Tampereen Teatterin Naisyhdistys RY:ssä

    OpenAIRE

    Mäntylä, Mirja

    2013-01-01

    Opinnäytetyö on tehty Tampereen Teatterin Naisyhdistys RY:lle ja Tampereen Teatteri Oy:lle. Tampereen Teatterin Naisyhdistys RY organisoi vapaaehtoistyötä kulttuurin hyväksi. Yhdistys toimii Tampereen Teatterissa miehittämällä Tampereen Teatterin palvelut Oy:n kahvilatoiminnan työvuoroja teatterinäytösten aikaisessa kahvilamyynnissä. Opinnäytetyön tarkoituksena on tutkia johtamisen työkalua, viestintää, Tampereen Teatterin Naisyhdistys RY:ssä toimivien ryhmäpäälliköiden näkökulmasta. Nykytila...

  10. Y-sukupolven johtaminen Airpro Oy:ssä

    OpenAIRE

    Lehtola, Heikki

    2014-01-01

    Y-sukupolvi on jo hämmentämässä työelämää. Näitä 1980−1990 -luvuilla syntyneitä ihmisiä kuvaillaan kärsimättömäksi sukupolveksi, joka ei auktoriteetteja kunnioita. Monissa yrityksissä ollaan hieman hämillään näiden nuorien työntekijöiden kanssa, sillä heitä tuntuvat motivoivan erilaiset asiat kuin aikaisempia sukupolvia. Airpro Oy:n turvatarkastusyksikkö muodostaa yrityksen liikevaihdollisesti suurimman yksikön. Sen 336 työntekijästä suurin osa kuuluu y-sukupolveen. Yrityksessä on huomatt...

  11. PCI-SS: MISO dynamic nonlinear protein secondary structure prediction

    Directory of Open Access Journals (Sweden)

    Aboul-Magd Mohammed O

    2009-07-01

    Full Text Available Abstract Background Since the function of a protein is largely dictated by its three dimensional configuration, determining a protein's structure is of fundamental importance to biology. Here we report on a novel approach to determining the one dimensional secondary structure of proteins (distinguishing α-helices, β-strands, and non-regular structures from primary sequence data which makes use of Parallel Cascade Identification (PCI, a powerful technique from the field of nonlinear system identification. Results Using PSI-BLAST divergent evolutionary profiles as input data, dynamic nonlinear systems are built through a black-box approach to model the process of protein folding. Genetic algorithms (GAs are applied in order to optimize the architectural parameters of the PCI models. The three-state prediction problem is broken down into a combination of three binary sub-problems and protein structure classifiers are built using 2 layers of PCI classifiers. Careful construction of the optimization, training, and test datasets ensures that no homology exists between any training and testing data. A detailed comparison between PCI and 9 contemporary methods is provided over a set of 125 new protein chains guaranteed to be dissimilar to all training data. Unlike other secondary structure prediction methods, here a web service is developed to provide both human- and machine-readable interfaces to PCI-based protein secondary structure prediction. This server, called PCI-SS, is available at http://bioinf.sce.carleton.ca/PCISS. In addition to a dynamic PHP-generated web interface for humans, a Simple Object Access Protocol (SOAP interface is added to permit invocation of the PCI-SS service remotely. This machine-readable interface facilitates incorporation of PCI-SS into multi-faceted systems biology analysis pipelines requiring protein secondary structure information, and greatly simplifies high-throughput analyses. XML is used to represent the input

  12. [Laboratory-based evaluation of "INOVA/QUANTA Lite" to determine antinuclear antibodies (ANA) and autoantibodies to double-stranded DNA, SS-A and SS-B].

    Science.gov (United States)

    Yamauchi, Megumi S; Shingaki, Naohiko; Yamane, Nobuhisa

    2012-05-01

    We evaluated QUANTA Lite reagent series (INOVA Diagnostics, CA, USA) to determine antinuclear antibodies (ANA) and autoantibodies to double-stranded (ds) DNA, SS-A and SS-B, in parallel with MESACUP (Medical & Biological Laboratories, Nagoya). Overall agreements between two reagents for qualitative interpretation ranged from 77.5% (ANA) to 99.0%(anti-SS-B antibodies). When we compared to the results by indirect fluorescent antibody (IFA) test on HEp-2 cells, QUANTA Lite ANA demonstrated better sensitivity and specificity; 92.2% versus 76.5% in sensitivity and 92.1% versus 86.8% in specificity. Also, determining anti-chromatin antibodies and IFA test onto Chrithidia luciliae demonstrated greater interpretive correlation to detect anti-ds DNA by QUANTA Lite than by MESACUP. All the discrepant sera to which QUANTA Lite SS-A gave positive interpretations were confirmed to contain the antibodies specific to SS-A 52kDa antigen, which is supplemented to QUANTA Lite capture-probes. With these results, we can conclude that QUANTA Lite has superiorities over MESACUP; (1) to detect a variety of autoantibodies consisting of ANA, (2) to have a better correlation with confirmatory tests to detect anti-ds DNA antibodies, (3)to detect additional autoantibodies specific to SS-A 52kDa antigen, and (4) to have an enough compatibility in determining anti-SS-B antibodies.

  13. RNA secondary structure mediates alternative 3'ss selection in Saccharomyces cerevisiae

    DEFF Research Database (Denmark)

    Plass, Mireya; Codony-Servat, Carles; Ferreira, Pedro Gabriel;

    2012-01-01

    that sequence and structure properties of the pre-mRNA could explain the selection of 3' splice sites (ss) in Saccharomyces cerevisiae. In this work, we extend our previous observations to build a computational classifier that explains most of the annotated 3'ss in the CDS and 5' UTR of this organism. Moreover......, we show that the same rules can explain the selection of alternative 3'ss. Experimental validation of a number of predicted alternative 3'ss shows that their usage is low compared to annotated 3'ss. The majority of these alternative 3'ss introduce premature termination codons (PTCs), suggesting...... selection in yeast mediated by the pre-mRNA structure, which can be responsive to external cues, like temperature, and is possibly related to the control of gene expression....

  14. GSM-moduuli sulautetussa järjestelmässä

    OpenAIRE

    Rantanen, Timo

    2011-01-01

    ssä opinnäytetyössä tutustuttiin GSM-moduulin ohjaukseen ja käyttöön sulautetussa järjestelmässä. Työssä käytettiin apuna Olimexin AVR-GSM-kehitysalustaa. Kehitysalustan tärkeimmät komponentit ovat ATMega32-mikrokontrolleri sekä Simcomin SIM300D GSM-moduuli. Työssä esitellään AVR-GSM-kehitysalusta ja SIM300D GSM-moduulin ohjaus AT-komennoilla. Työssä tehtiin myös esimerkkisovellus, joka havainnollistaa kehitysalustan ja GSM-moduulin käyttöä ja ohjausta. Opinnäytetyön ohjelmistokehitys t...

  15. Effect of the Medicinal Agaricus blazei Murill-Based Mushroom Extract, AndoSanTM, on Symptoms, Fatigue and Quality of Life in Patients with Crohn’s Disease in a Randomized Single-Blinded Placebo Controlled Study

    Science.gov (United States)

    Hetland, Geir; Lyberg, Torstein; Lygren, Idar; Johnson, Egil

    2016-01-01

    Background Ingestion of AndoSanTM, based on the mushroom Agaricus blazei Murill, has previously shown an anti-inflammatory effect through reduction of pro-inflammatory cytokines in healthy individuals and patients with Crohn’s disease (CD). In this randomized single-blinded placebo-controlled study we examined whether intake of AndoSanTM also resulted in clinical effects. Methods and Findings 50 patients with symptomatic CD were randomized for oral daily consumption of AndoSanTM or placebo for a 21-day experimental period, in this per-protocol study. Patients reported validated scores for symptoms, fatigue and health related quality of life (HRQoL) at days 0, 14 and 21. Fecal calprotectin and general blood parameters were also analyzed. In the AndoSanTM group (n = 25) symptoms improved from baseline (day 0) to days 14 and 21, with respective mean scores (95% CI) of 5.52 (4.64–6.40), 4.48 (3.69–5.27) and 4.08 (3.22–4.94) (p<0,001). We found significant improvements in symptom score for both genders in the AndoSanTM group, and no significant changes in the placebo (n = 25) group. There were however no significant differences between the groups (p = 0.106), although a marginal effect in symptom score for men (p = 0.054). There were comparable improvements in physical, mental and total fatigue for both groups. HRQoL versus baseline were at day 21 improved for bodily pain and vitality in the AndoSanTM group and for vitality and social functioning in the placebo group. No crucial changes in general blood samples and fecal calprotectin were detected. Conclusions The results from this single-blinded randomized clinical trial shows significant improvement on symptoms, for both genders, in the AndoSanTM group, but no significant differences between the study groups. The results on fatigue, HRQoL, fecal calprotectin and blood samples were quite similar compared with placebo. The patients did not report any harms or unintended effects of AndoSanTM. CD patients with

  16. Synthesis and characterization of PEG-P(MAA-SS-VCL) nanoparticles

    Science.gov (United States)

    Yu, L. L.; Yang, K.; Mu, R. H.; Zhang, N.; Su, L.

    2016-07-01

    The PEG-P(MAA-SS-VCL) nanoparticles were obtained using disulfide containing dimethacrylate (SS) as cross-linking agent, using polyethylene glycol methyl acrylate (PEGMA), N-Vinyl-ε-caprolactam (VCL), and methacrylic acid (MAA) as monomers via homogeneous polymerization in aqueous. The PEG-P(MAA-SS-VCL) nanoparticles were characterized by FT-IR and TGA. The particle size and morphology variation in different environments were detected by dynamic light scattering (DLS) and scanning electron microscopy (SEM). It is the very method that PEG-P(MAA-SS-VCL) nanoparticles can be obtained in this study.

  17. Työssäoppimisenohjauksen manuaali : kehitystyö Hotelli Hiltonissa

    OpenAIRE

    Kiviö, Johanna

    2015-01-01

    Tämän opinnäytetyön tavoitteena oli kehittää työssäoppimisen ohjaajille opas opiskelijoiden ohjaamiseen. Opas toteutettiin infografin muodossa. Tutkimuksessa tarkasteltiin työssäoppimisen teoreettisia perusteita, valotettiin millainen on työpaikka oppimisympäristönä ja avattiin työssäoppimisen ohjauksen käsitteitä̈. Lisäksi käsiteltiin hiljaisen tiedon olemusta kokin ammatissa. Kokoavana teoriana esiteltiin Myrkän ja Hätösen ja Friskin ideaalimalli työssäoppimisen ohjaukseen. Kehitys...

  18. Varaosasovelluksen suunnittelu ja toteutus ASP.NET-ympäristössä

    OpenAIRE

    Rauhala, Henri

    2015-01-01

    ssä opinnäytetyössä kerrotaan varaosasovelluksen suunnittelusta ja toteutuksesta ASP.NET-ympäristössä Saides Engineering Oy:n asiakkaalle. Kyseisellä asiakkaalla oli ennen projektin aloitusta aiempi versio web-pohjaisesta varaosasovelluksesta, jota haluttiin nyt kehittää kauppapaikan suuntaan. Opinnäytetyössä käydään läpi uuden sovelluksen suunnittelua, ominaisuuksia sekä sovelluksen ohjelmointiratkaisuja. Projektin kehitystiimi koostui itsestäni sekä Saides Engineering Oy:n toimitusjoh...

  19. Analytical Investigation and Comparison on Performance of Ss316, Ss440c and Titanium Alloy Tool Materials Used As Single Point Cutting Tool

    Directory of Open Access Journals (Sweden)

    Mr. Amaresh Kumar Dhadange

    2015-08-01

    Full Text Available Theoretical analysis for performance studies of SS316, SS44OC and Titanium Alloy used as a cutting tool is presented in this paper. Tool temperature, tools wear and life of the tool is investigated analytically. These theoretical values are compared with the experimental studies conducted by the author. The values obtained from experimental studies are comparable with analytical values and variation is the correlation between theoretical and experimental values is of the order of 15%.

  20. Jets of SS433 on scales of dozens parsecs

    CERN Document Server

    Panferov, Alexander A

    2016-01-01

    The radio nebula W50 harbours the relativistic binary system SS433, which is a source of the powerful wind and jets. The origin of W50 is wrapped in the interplay of the wind, supernova remnant and jets. The evolution of the jets on the scales of the nebula is a Rosetta stone for its origin. To disentangle the roles of these components, we study physical conditions of the jets propagation inside W50, and determine deceleration of the jets. The morphology and parameters of the interior of W50 are analyzed using the available observations of the eastern X-ray lobe, which traces the jet. In order to estimate deceleration of this jet, we devised a simplistic model of the viscous interaction, via turbulence, of a jet with the ambient medium, which would fit mass entrainment from the ambient medium into the jets of the radio galaxy 3C31, the well studied case of continuously decelerating jets. X-ray observations suggest that the eastern jet persists through W50 as hollow one, and is recollimated to the opening $\\si...

  1. More on the circumbinary disk of SS 433

    CERN Document Server

    Bowler, M G

    2010-01-01

    Certain lines in spectra of the Galactic microquasar SS 433, in particular the brilliant H alpha line, have been interpreted as emission from a circumbinary disk. In this interpretation the orbital speed of the glowing material is in excess of 200 km/s and the mass of the binary system in excess of 40 solar masses. A very simple model of excitation of disk material is in remarkable agreement with the observations, yet it seems that the very existence of a circumbinary disk is regarded as controversial. Published spectra, taken almost nightly over two orbital periods of the binary system, show H alpha and He I lines; these were analysed as superpositions of Gaussian components. A model in which the excitation of any given patch of putative circumbinary material is proportional to the inverse square of its instantaneous distance from the compact object was constructed and compared with observations. The new model provides an excellent description of the observations. The variation of the H alpha and He I spectr...

  2. Interpretation of observations of the circumbinary disk of SS 433

    CERN Document Server

    Bowler, M G

    2010-01-01

    Context. The Galactic microquasar SS 433 is possessed of a circumbinary disk most clearly seen in the brilliant Balmer H alpha emission line. The orbital speed of the glowing material is an important determinant of the mass of the binary system. The circumbinary disk may be fed through the L2 point and in turn may feed a very extended radio feature known as the ruff. Aims. To present an analysis of spectroscopic optical data from H alpha and He I spectral lines which reveal the circumbinary disk. To use comparisons of the rather different signals to better understand the disk and improve estimates of the rotational speed of the inner rim. To present a simple model which naturally explains some apparently bizarre spectral variations with orbital phase. Methods. Published spectra, taken almost nightly over two orbital periods of the binary system, are analysed. H alpha and He I lines are analysed as superpositions of Gaussian components and a simple model constructed. Results. The data are understood in terms o...

  3. On the bizarre gamma-ray spectrum of SS 433

    Science.gov (United States)

    Helfer, H. L.; Savedoff, M. P.

    1984-01-01

    Lamb et al. (1983) have announced the discovery of a pair of gamma-ray lines interpretable as emission of the 1.368 MeV line of Mg-24 in the two oppositely directed relativistic jets of SS 433. The mass loss rate related to the Mg-24 and the kinetic energy flux of the Mg-24 are considered. In the present investigation, it is shown that the mass loss flux must be well in excess of 0.00001 solar mass per yr, while the abundance of the gamma-emitting nucleus is extremely high. Attention is given to the calculation of the gamma-ray production efficiency factor, the size of the emitting region, reaction processes, and X-ray luminosity. It is concluded that for plasma beam models, there must be a substantial overabundance, by a factor of 100 to approximately 1000, of the gamma-line producing nucleus. The association of the gamma-ray lines with Mg-24 is reasonable but not secure.

  4. S-S bond reactivity in metal-perthiocarboxylato compounds.

    Science.gov (United States)

    Mas-Ballesté, Rubén; Guijarro, Alejandro; González-Prieto, Rodrigo; Castillo, Oscar; Sanz Miguel, Pablo J; Zamora, Félix

    2010-02-14

    While M-percarboxylato species are elusive intermediates, their sulfur containing analogues are known in some cases. The feasibility of isolation of M-perthioacetato compounds allowed, in this work, to obtain new insights into the pathways that follow the reactivity of M-E-ER (E = O, S) fragments. Herein we report on the isolation of two new M-perthioacetato compounds: trans-[Pt(CH(3)CS(2)S)(2)] () and [Ni(CH(3)CSS)(CH(3)CS(2)S)] (), which have been fully characterized, including X-ray structures. Reactivity of these compounds towards PPh(3) has been studied combining UV-vis monitorization and NMR measurements. Overall the accumulated data suggest that the evolution of the perthioacetato ligand in complexes and by reaction with PPh(3) consists of a complex multistep pathway in which the sulfur transfer is preceded by electron transfer. Cyclic voltammetry measurements indicate that the transference of two electrons from the phosphorus to the sulfur atom is not concerted, suggesting that the first step of the reaction with PPh(3) is the monoelectronic electron transfer followed by P-S bond formation. The results presented here show a novel pathway in the field of S-S bond reactivity processes relevant in biological, synthetic systems and in hydrocarbon desulfurization processes.

  5. Laser Welding of Ultra-Fine Grained Steel SS400

    Institute of Scientific and Technical Information of China (English)

    PENG Yun; TIAN Zhi-ling; CHEN Wu-zhu; WANG Cheng; BAO Gang

    2003-01-01

    The effects of laser welding on microstructure and mechanical properties of ultra-fine grained steel SS400 were studied. The plasma arc welding and MAG welding were conducted to make a comparison between these weldings and laser welding. The coarse grain heat-affected zone (HAZ) of laser welding was simulated using thermomechanical simulation machine, and the impact toughness was tested. The deep penetration laser welding produces weld of large depth and narrow width. The weld metal and HAZ of laser welding was heated and then cooled rapidly. The prior austenite grain size of coarse grain HAZ is 1/10 of that for arc welding. For laser welding, the toughness of weld metal is higher than that of base metal, and the toughness of the coarse grain HAZ of laser welding is on a level with that of base metal. Matching lower laser power with lower welding speed, the hardening tendency of the weld metal and the coarse grain HAZ can be decreased. There is no softened zone. The tensile strength of welded joint formed by laser is higher than that of base metal. The joint has good bending ductility.

  6. Evolution of microstructure in laser welding of SS304L

    International Nuclear Information System (INIS)

    Laser welding is an important joining process and its application in industries is growing rapidly. One can produce laser welds over a wide range of process parameters and this offers very good opportunity for producing microstructure of different morphology and scales in the weldment. Weld beads have been produced on 5 mm thick plates of SS304L using CW Nd-YAG laser. Laser power was varied in 200 W to 1000 W range and welding speed was varied in 100 mm/mm to 1000 mm/mm. This resulted in weld beads of different morphology. Microstructure of the weld beads was examined on the cross-section as well as in the axial direction using optical microscopy and Transmission Electron Microscopy (TEM) to study evolution of the microstructure in the weldment. Microstructure was cellular and cellular-dendritic with grains growing from the fusion line towards the centerline. In the central region, cellular growth along the welding direction was observed. The cell size was found to increase with increasing laser power and decreasing welding speed. The findings are presented in this paper. (author)

  7. Interfacial Shear Strength of Oxide Scale and SS 441 Substrate

    Science.gov (United States)

    Liu, Wenning; Sun, Xin; Stephens, Elizabeth; Khaleel, Moe

    2011-05-01

    Recent developments on decreasing the operating temperature for solid oxide fuel cells (SOFCs) have enabled the use of high-temperature ferritic alloys as interconnect materials. Oxide scale will inevitably grow on the ferritic interconnects in a high-temperature oxidation environment of SOFCs. The growth of the oxide scale induces growth stresses in the scale layer and on the scale/substrate interface. These growth stresses combined with the thermal stresses induced after stacking cooling by the thermal expansion coefficient mismatch between the oxide scale and the substrate may lead to scale delamination/buckling and eventual spallation, which may lead to serious cell performance degradation. Hence, the interfacial adhesion strength between the oxide scale and the substrate is crucial to the reliability and durability of the metallic interconnect in SOFC operating environments. In this article, we applied an integrated experimental/modeling methodology to quantify the interfacial adhesion strength between the oxide scale and the SS 441 metallic interconnect. The predicted interfacial strength is discussed in detail.

  8. Interfacial Shear Strength of Oxide Scale and SS 441 Substrate

    Energy Technology Data Exchange (ETDEWEB)

    Liu, Wenning N.; Sun, Xin; Stephens, Elizabeth V.; Khaleel, Mohammad A.

    2011-05-01

    Recent developments on decreasing the operating temperature for Solid Oxide Fuel Cells (SOFCs) have enabled the use of high temperature ferritic alloys as interconnect materials. Oxide scale will inevitably grow on the ferritic interconnects in a high temperature oxidation environment of SOFCs. The growth of the oxide scale induces growth stresses in the scale layer and on the scale/substrate interface. These growth stresses combined with the thermal stresses induced upon stacking cooling by the thermal expansion coefficient mismatch between the oxide scale and the substrate may lead to scale delamination/buckling and eventual spallation, which may lead to serious cell performance degradation. Hence the interfacial adhesion strength between the oxide scale and the substrate is crucial to the reliability and durability of the metallic interconnect in SOFC operating environments. In this paper, we applied an integrated experimental/modeling methodology to quantify the interfacial adhesion strength between the oxide scale and the SS 441 metallic interconnect. The predicted interfacial strength is discussed in details.

  9. SS 433: Results of a Recent Multi-wavelength Campaign

    CERN Document Server

    Chakrabarti, S K; Pal, S; Mondal, S A; Nandi, A; Bhattacharya, A; Mandal, S; Sagar, R; Pandey, J C; Pati, A; Saha, S K; Chakrabarti, Sandip K.; Mondal, Soumen; Mandal, Samir; Sagar, Ram

    2005-01-01

    We conducted a multi-wavelength campaign in September-October, 2002, to observe SS 433. We used 45 meter sized 30 dishes of Giant Meter Radio Telescope (GMRT) for radio observation, 1.2 meter Physical Research Laboratory Infra-red telescope at Mt Abu for IR, 1 meter Telescope at the State Observatory, Nainital for Optical photometry, 2.3 meter optical telescope at the Vainu Bappu observatory for spectrum and Rossi X-ray Timing Explorer (RXTE) Target of Opportunity (TOO) observation for X-ray observations. We find sharp variations in intensity in time-scales of a few minutes in X-rays, IR and radio wavelengths. Differential photometry at the IR observation clearly indicated significant intrinsic variations in short time scales of minutes throughout the campaign. Combining results of these wavelengths, we find a signature of delay of about two days between IR and Radio. The X-ray spectrum yielded double Fe line profiles which corresponded to red and blue components of the relativistic jet. We also present the b...

  10. Tritium release from SS316 under vacuum condition

    Energy Technology Data Exchange (ETDEWEB)

    Torikai, Y.; Penzhorn, R.D. [Hydrogen Isotope Research Center, University of Toyama, Toyama (Japan)

    2015-03-15

    The plasma facing surface of the ITER vacuum vessel, partly made of low carbon austenitic stainless steel type 316L, will incorporate tritium during machine operation. In this paper the kinetics of tritium release from stainless steel type 316 into vacuum and into a noble gas stream are compared and modelled. Type 316 stainless steel specimens loaded with tritium either by exposure to 1.2 kPa HT at 573 K or submersion into liquid HTO at 298 K showed characteristic thin surface layers trapping tritium in concentrations far higher than those determined in the bulk. The evolution of the tritium depth profile in the bulk during heating under vacuum was non-discernible from that of tritium liberated into a stream of argon. Only the relative amount of the two released tritium-species, i.e. HT or HTO, was different. Temperature-dependent depth profiles could be predicted with a one-dimensional diffusion model. Diffusion coefficients derived from fitting of the tritium release into an evacuated vessel or a stream of argon were found to be (1.4 ± 1.0)*10{sup -7} and (1.3 ± 0.9)*10{sup -9} cm{sup 2}/s at 573 and 423 K, respectively. Polished surfaces on type SS316 stainless steel inhibit considerably the thermal release rate of tritium.

  11. Corrosion erosion test of SS316 in flowing Pb Bi

    Science.gov (United States)

    Kikuchi, K.; Kurata, Y.; Saito, S.; Futakawa, M.; Sasa, T.; Oigawa, H.; Wakai, E.; Miura, K.

    2003-05-01

    Corrosion tests of austenitic stainless tube were done under flowing Pb-Bi conditions for 3000 h at 450 °C. Specimens were 316SS produced as a tubing form with 13.8 mm outer diameter, 2 mm thickness and 40 cm length. During operation, maximum temperature, temperature difference and flow velocity of Pb-Bi at the specimen were kept at 450, 50 °C, and 1 m/s, respectively. After the test, specimen and components of the loop were cut and examined by optical microscope, SEM, EDX, WDX and X-ray diffraction. Pb-Bi adhered on the surface of the specimen even after Pb-Bi was drained out to the storage tank from the circulating loop. Results differed from a stagnant corrosion test in that the specimen surface became rough and the corrosion rate was maximally 0.1 mm/3000 h. Mass transfer from the high temperature to the lower temperature area was observed: crystals of Fe-Cr were found on the tube surface in the low-temperature region. The sizes of crystals varied from 0.1 to 0.2 mm. The depositing crystals were ferrite grains and the chemical composition ratio (mass%) of Fe to Cr was 9:1.

  12. Identification of the Mass Donor Star's Spectrum in SS 433

    CERN Document Server

    Hillwig, T C; Huang, W; McSwain, M V; Stark, M A; Van der Meer, Alex F G; Kaper, L

    2004-01-01

    We present spectroscopy of the microquasar SS 433 obtained near primary eclipse and disk precessional phase Psi = 0.0, when the accretion disk is expected to be most ``face-on''. The likelihood of observing the spectrum of the mass donor is maximized at this combination of orbital and precessional phases since the donor is in the foreground and above the extended disk believed to be present in the system. The spectra were obtained over four different runs centered on these special phases. The blue spectra show clear evidence of absorption features consistent with a classification of A3-7 I. The behavior of the observed lines indicates an origin in the mass donor. The observed radial velocity variations are in anti-phase to the disk, the absorption lines strengthen at mid-eclipse when the donor star is expected to contribute its maximum percentage of the total flux, and the line widths are consistent with lines created in an A supergiant photosphere. We discuss and cast doubt on the possibility that these line...

  13. 27 CFR 40.171 - Execution and filing of Form SS-4.

    Science.gov (United States)

    2010-04-01

    ... applications may be filed with the district director of any such district as provided for in 26 CFR 301.6091-1... Form SS-4. 40.171 Section 40.171 Alcohol, Tobacco Products and Firearms ALCOHOL AND TOBACCO TAX AND... Payment of Taxes on Tobacco Products § 40.171 Execution and filing of Form SS-4. The application on...

  14. Some inequalities of Ostrowski and Grüss type for triple integrals on time scales

    Directory of Open Access Journals (Sweden)

    Nazir Ahmad Mir

    2011-12-01

    Full Text Available In this paper, we establish some inequalities of Ostrowski and Grüss type for triple integrals on arbitrary time scales involving three functions and their partial derivatives. We also discuss the discrete Ostrowski and Grüss type inequalities for triple sumon time scale.

  15. Some inequalities of Ostrowski and Grüss type for triple integrals on time scales

    OpenAIRE

    Nazir Ahmad Mir; Roman Ullah

    2011-01-01

    In this paper, we establish some inequalities of Ostrowski and Grüss type for triple integrals on arbitrary time scales involving three functions and their partial derivatives. We also discuss the discrete Ostrowski and Grüss type inequalities for triple sumon time scale.

  16. Metabolic and transcriptomic changes induced in Arabidopsis by the rhizobacterium Pseudomonas fluorescens SS101

    NARCIS (Netherlands)

    Mortel, van de J.E.; Vos, de R.C.H.; Dekkers, E.; Pineda, A.; Guillod, L.; Bouwmeester, K.; Loon, van J.J.A.; Dicke, M.; Raaijmakers, J.M.

    2012-01-01

    Systemic resistance induced in plants by nonpathogenic rhizobacteria is typically effective against multiple pathogens. Here, we show that root-colonizing Pseudomonas fluorescens strain SS101 (Pf.SS101) enhanced resistance in Arabidopsis (Arabidopsis thaliana) against several bacterial pathogens, in

  17. Psychometric Reevaluation of the Women in Science Scale (WiSS)

    Science.gov (United States)

    Owen, Steven V.; Toepperwein, Mary Anne; Pruski, Linda A.; Blalock, Cheryl L.; Liu, Yan; Marshall, Carolyn E.; Lichtenstein, Michael J.

    2007-01-01

    The Women in Science Scale (WiSS) was first developed in 1984, and is still being used in contemporary studies, yet its psychometric properties have not been evaluated with current statistical methods. In this study, the WiSS was administered in its original 27-item form to 1,439 middle and high school students. Confirmatory factor analysis based…

  18. IRC:n käyttö yritysten viestinnässä

    OpenAIRE

    Klami, Maarit

    2012-01-01

    ssä työssä perehdyttiin IRC:n käyttömahdollisuuksiin yritysten sisäisessä ja ulkoisessa viestinnässä. Työssä verrattiin IRC:tä muihin pikaviestimiin sekä sosiaalisen median tarjoamiin viestintä- ja markkinointimahdollisuuksiin. Eräs tarkastelluista seikoista on yrityksen koon vaikutus siihen, kuinka mittava operaatio IRC:n käyttöönotto yrityksessä on. Työssä on esitetty myös mahdollisuuksia muiden pikaviestimien integroimiseksi IRC:hen. Työ on tehty mielenkiinnosta käyttää IRC:tä viestinnän...

  19. Mitochondria-targeted peptide SS-31 attenuates renal injury via an antioxidant effect in diabetic nephropathy.

    Science.gov (United States)

    Hou, Yanjuan; Li, Shuangcheng; Wu, Ming; Wei, Jinying; Ren, Yunzhuo; Du, Chunyang; Wu, Haijiang; Han, Caili; Duan, Huijun; Shi, Yonghong

    2016-03-15

    Oxidative stress is implicated in the pathogenesis of diabetic kidney injury. SS-31 is a mitochondria-targeted tetrapeptide that can scavenge reactive oxygen species (ROS). Here, we investigated the effect and molecular mechanism of mitochondria-targeted antioxidant peptide SS-31 on injuries in diabetic kidneys and mouse mesangial cells (MMCs) exposed to high-glucose (HG) ambience. CD-1 mice underwent uninephrectomy and streptozotocin treatment prior to receiving daily intraperitoneal injection of SS-31 for 8 wk. The diabetic mice treated with SS-31 had alleviated proteinuria, urinary 8-hydroxy-2-deoxyguanosine level, glomerular hypertrophy, and accumulation of renal fibronectin and collagen IV. SS-31 attenuated renal cell apoptosis and expression of Bax and reversed the expression of Bcl-2 in diabetic mice kidneys. Furthermore, SS-31 inhibited expression of transforming-growth factor (TGF)-β1, Nox4, and thioredoxin-interacting protein (TXNIP), as well as activation of p38 MAPK and CREB and NADPH oxidase activity in diabetic kidneys. In vitro experiments using MMCs revealed that SS-31 inhibited HG-mediated ROS generation, apoptosis, expression of cleaved caspase-3, Bax/Bcl-2 ratio, and cytochrome c (cyt c) release from mitochondria. SS-31 normalized mitochondrial potential (ΔΨm) and ATP alterations, and inhibited the expression of TGF-β1, Nox4, and TXNIP, as well as activation of p38 MAPK and CREB and NADPH oxidase activity in MMCs under HG conditions. SS-31 treatment also could reverse the reduction of thioredoxin (TRX) biologic activity and upregulate expression of thioredoxin 2 (TRX2) in MMCs under HG conditions. In conclusion, this study demonstrates a protective effect of SS-31 against HG-induced renal injury via an antioxidant mechanism in diabetic nephropathy.

  20. SS400含硼钢板坯边裂研究%Research on edge cracking of SS4 0 0 boron-bearing steel slab

    Institute of Scientific and Technical Information of China (English)

    龙小东; 刘小卿; 张亮

    2014-01-01

    In this paper,high temperature brittle zone of SS400 boron-bearing steel were determined among experiment temperature range of 650~1 150 ℃ through the thermal simulation experiment of slab.Then the main reasons of forming edge cracks were re-vealed through high temperature strength and thermoplastic analysis of the slab,which involved the components of SS400 boron-bearing steel,which is in the range of peritectic steels,and the BN precipitation on grain boundary.During the practical production process of SS400 boron-bearing steel,the proposed measure can significantly reduce the edge cracks on slab surface.%经过高温力学性能实验,在650~1150℃的实验温度范围内明确了 SS400含硼钢的高温脆性区;并通过对铸坯的高温强度和热塑性分析,揭示了铸坯边裂产生的主要原因是钢种成分处于包晶钢的范围和钢中BN的析出。在 SS400含硼钢实际生产中,运用本文提出的措施可显著减少铸坯边裂的发生。

  1. Species differences in distribution and prediction of human V(ss) from preclinical data.

    Science.gov (United States)

    Berry, Loren M; Li, Chao; Zhao, Zhiyang

    2011-11-01

    Prediction of human volume of distribution at steady state (V(ss)) before first administration of a new drug candidate to humans has become an important part of the drug development process. This study examines the assumptions behind interspecies scaling techniques used to predict human V(ss) from preclinical data, namely the equivalency of V(ss,u) and/or f(ut) across species. In addition, several interspecies scaling techniques are evaluated side by side using a set of 67 reference compounds where observed V(ss) from rats, dogs, monkeys, and humans were compiled from the literature and where plasma protein binding was determined across species using an ultracentrifugation technique. Species similarity in V(ss,u) or f(ut) does not appear to be the norm among rats, dogs, monkeys, or humans. Despite this, interspecies scaling from rats, dogs, and monkeys is useful and can provide reasonably accurate predictions of human V(ss), although some interspecies scaling approaches were better than others. For example, the performance of the common V(ss,u) or f(ut) equivalency approaches using average V(ss,u) or f(ut) across three preclinical species was superior to allometric scaling techniques. In addition, considering data from several preclinical species, using the equivalency approach, was superior to scaling from any single species. Although the mechanistic tissue composition equations available in the Simcyp population-based pharmacokinetic simulator did not necessarily provide the most accurate predictions, and the equations used likely need refinement, they still provide the best opportunity for a mechanistic understanding and prediction of human V(ss).

  2. Monitoring and analyzing system based on SS7 network%基于SS7信令网的监测分析系统

    Institute of Scientific and Technical Information of China (English)

    杨安萍; 张宇; 郭炜强

    2006-01-01

    现代各种通信网络的网内和网间信令系统大多是七号信令(SS7)系统,其对于通信网的服务质量和服务水平起着非常重要的作用.随着SS7信令网复杂性的增加,需要对信令网进行集中监测以及分析.针对此情况,提出一种基于SS7信令网的监测分析系统的设计以及实现.运用了分布式和模块化的设计,在实际应用中取得较好的效果.

  3. Responsiivinen web-kehitys WordPress-julkaisujärjestelmässä

    OpenAIRE

    Jutila, Tiina

    2013-01-01

    Opinnäytetyössä tutkittiin responsiivisen web-kehityksen toteuttamista WordPress-julkaisujärjes¬-telmässä. Tavoitteena oli toteuttaa yrityksen käyttöön WordPress-teema, joka sisälsi tarvittavat ominaisuudet mukautuvien verkkosivujen toteuttamiseen. Teeman tarkoituksena oli toimia pohjana, jonka päälle verkkosivun rakentaminen olisi mahdollisimman vaivatonta. Työssä käsiteltiin mobiililaitteiden asettamia haasteita web-kehityksessä, sekä tekniikoita niiden rat-kaisemiseen. Päätelaitteiden ...

  4. Synthesis and electrochemical characterization of porous niobium oxide coated 316L SS for orthopedic applications

    International Nuclear Information System (INIS)

    Niobium oxide was prepared using sol-gel process and coated on 316L stainless steel (SS) substrate via dip-coating technique. The surface characterization results after a thermal treatment revealed that the coated surface was porous, uniform and well crystalline on the substrate. The corrosion resistance and bioactivity of the porous niobium oxide coated 316L SS in simulated body fluid (SBF) solution was evaluated. The in vitro test revealed a layer of carbonate-containing apatite formation over the coated porous surface. The results indicated that the porous niobium oxide coated 316L SS exhibited a high corrosion resistance and an enhanced biocompatibility in SBF solution.

  5. Liikkeenharjoittaja vuokralaisena asunto-osakeyhtiössä sijaitsevassa liiketilassa - osapuolten välinen vastuunjako

    OpenAIRE

    Santasalo, Hanna

    2014-01-01

    Diplomityössä tutkittiin sitä, millainen on lainsäädännön mukainen vastuunjako osapuolten välillä silloin, kun liiketoiminnanharjoittaja on vuokralaisena asunto-osakeyhtiössä sijaitsevassa liiketilassa. Lisäksi selvitettiin tyypillisimpiä ongelma- ja riitatilanteita eri osapuolten välillä. Teoreettisen tutkimuksen lisäksi tavoitteena oli luoda Suomen Lähikauppa Oy:lle asunto-osakeyhtiön erityispiirteet huomioiva vuokrasopimusmalli sekä asunto-osakeyhtiössä sijaitsevan liiketilan vuokraukseen ...

  6. Suunnitelma työssäoppimisblogin käyttöönottoon

    OpenAIRE

    Järvenpää, Pauliina

    2015-01-01

    Tämän opinnäytetyön tarkoitus oli selvittää ammatillisen toisen asteen opiskelijoiden tarpeita, toiveita ja haluja siirtyä työssäoppimisvihkosta ajanmukaiseen sähköisen viestinnän välineeseen työssäoppimisblogiin. Viestintä- ja mediaosaaminen sekä teknologia ja tietotekniikka ovat ammatillisen toisen asteen oppilaitoksissa opetussuunnitelmassa ammattitaitovaatimuksissa arvioinnin kohteina, johon sähköinen viestinnän väline blogi sopii erityisen hyvin. Kehittämistyössä teoriaosuus palvelumuoto...

  7. The Testing Apparatus for SS7 Electric Locomotive double-heading%SS7型电力机车重联试验装置

    Institute of Scientific and Technical Information of China (English)

    罗勇

    2010-01-01

    南宁机务段为解决SS7型电力机车经常发生重联故障,库内机车重联试验困难的问题,研制开发了SS7型电力机车重联试验装置.详细介绍了该装置供电电路、调制解调器、重联控制电路及显示屏的构成、原理及功能.

  8. Ääniä päässä : Äänisuunnitelman tekeminen teoriassa ja käytännössä

    OpenAIRE

    Hulkko, Teemu

    2015-01-01

    Tutkin opinnäytetyössäni äänisuunnittelijan roolia tuotannossa sekä äänisuunnitelman toteutusta teoriassa ja käytännössä. Tavoitteenani oli kirjoittaa havainnollistava teos äänisuunnittelusta ja sen mahdollisuuksista osana elokuvaa. Työ olen toteuttanut tutustumalla äänisuunnittelusta kertovaan kirjallisuuteen ja poiminut kohtauksia esimerkeiksi kuudesta eri elokuvasta. Kootun aineiston pohjalta kirjoitetun opinnäytetyön teoriaosan perusteella olen tehnyt äänisuunnitelman Keijujen koti -l...

  9. Klusterin toiminnan tutkiminen Linux-ympäristössä

    OpenAIRE

    Paavola, Jussi

    2010-01-01

    Työssä on tarkoitus tutkia, että minkälaisia klusterointimahdollisuuksia on tällä hetkellä tarjolla Linux-ympäristöön. Työssä käydään lyhyesti läpi Linux käyttöjärjestelmää ja klusterointia yleisesti. Tarkemmin työssä tutustutaan erilaisiin vaihtoehtoihin, mitä Linuxille on tarjolla klusterointiin. Klusteroinnin yhteydessä, tutkitaan myös hieman pilvitekniikkaa. Työssä rakennetaan myös oma klusteri koulun tietoliikenne-laboratoriossa. The purpose of this thesis was to explore what kin...

  10. Facebookin hyödyntäminen koripalloseurojen viestinnässä

    OpenAIRE

    Siven, Kim

    2012-01-01

    ssä opinnäytetyössä selvitetään Facebookin roolia koripalloseurojen viestinnässä. Facebook ja koripallo ovat molemmat tällä hetkellä ajankohtaisia aiheita. Työssä perehdytään myös sosiaalisen median hyötyihin yritykselle sekä asiakkaalle. Opinnäytetyö tehtiin syksyn 2011 ja kevään 2012 välisenä aikana. Työn tarkoitus on tutkia käyttävätkö seurat Facebookia ja millä tavoin ne ovat yhteydessä kuluttajiin. Lisäksi selvitetään käyttävätkö koripalloseurat koko Facebookin kapasiteettia markki...

  11. Changes of plasma SS, SP contents in adult patients with primary hypothyroidism

    International Nuclear Information System (INIS)

    Objective: To investigate the possible mechanism of imparirment of central nervous system function in hypothyroid patients through determination of changes of plasma neuropeptides after thyroid hormone replacement therapy. Methods: Plasma somatostatin (SS) and substance P(SP) contents were measured with RIA in 45 patients with primary hypothyroidism both before and after thyroid hormone replacement therapy as well as in 38 controls. Results: Before treatment, the plasma contents of SP in the patients were significantly lower than those in the controls (P0.05). However, the plasma contents of SS in the more advanced hypothyroid patients with FT33, FT4 levels, the plasma SS, SP increased significantly (vs before treatment P<0.05, P<0.01). Conclusion: The decrease of plasma contents of SS and SP in patients with hypothyroidism might be related to the development of psycho-neurological symptoms in these patients and thyroid hormone replacement therapy was desirable. (authors)

  12. Bee Wax Propolis Extract as Eco-Friendly Corrosion Inhibitors for 304SS in Sulfuric Acid

    Directory of Open Access Journals (Sweden)

    Femiana Gapsari

    2015-01-01

    Full Text Available The inhibition properties of bee wax propolis (BWP extract on the 304SS in 0.5 M sulfuric acid were conducted using potentiodynamic polarization, EIS, and XRD. Quercetin (2-(3.4-dihydroxy phenyl-3.5.7-trihydroxy-4H-chromen-4-one was identified as the main compound in the BWP extract based on FTIR and HPLC analysis. The results showed that the inhibitor could retard the corrosion rate of 304SS in 0.5 M sulfuric acid which reached 97.29% and 91.42% at 2000 ppm based on potentiodynamic polarization and EIS measurement, respectively. The inhibition efficiency decreased with increasing temperature. The inhibition mechanism of BWP extract on the 304SS was physisorption and obeyed Temkin adsorption isotherm equation. The thin protective layer on the 304SS surface was confirmed by XRD.

  13. As-rigid-as-possible mosaicking and serial section registration of large ssTEM datasets

    OpenAIRE

    Saalfeld, Stephan; Cardona, Albert; Hartenstein, Volker; Tomančák, Pavel

    2010-01-01

    Motivation: Tiled serial section Transmission Electron Microscopy (ssTEM) is increasingly used to describe high-resolution anatomy of large biological specimens. In particular in neurobiology, TEM is indispensable for analysis of synaptic connectivity in the brain. Registration of ssTEM image mosaics has to recover the 3D continuity and geometrical properties of the specimen in presence of various distortions that are applied to the tissue during sectioning, staining and imaging. These includ...

  14. Toxicity of Neurons Treated with Herbicides and Neuroprotection by Mitochondria-Targeted Antioxidant SS31

    OpenAIRE

    Hemachandra Reddy, P.; Byung Park; Ulziibat Shirendeb; Reddy, Arubala P.; Calkins, Marcus J.; Peizhong Mao; Reddy, Tejaswini P.; Maria Manczak

    2011-01-01

    The purpose of this study was to determine the neurotoxicity of two commonly used herbicides: picloram and triclopyr and the neuroprotective effects of the mitochondria-targeted antioxidant, SS31. Using mouse neuroblastoma (N2a) cells and primary neurons from C57BL/6 mice, we investigated the toxicity of these herbicides, and protective effects of SS1 peptide against picloram and triclopyr toxicity. We measured total RNA content, cell viability and mRNA expression of peroxiredoxins, neuroprot...

  15. 浅谈SS7型机车再生制动

    Institute of Scientific and Technical Information of China (English)

    李瑜

    2013-01-01

    本文主要对SS7型机车的再生制动进行了分析,对它的原理及调节过程进行了初步的介绍和分析,为更好的理解和掌握SS7型机车的主电路及控制电路奠定坚实的基础.

  16. Integrated Composite Stiffener Structure (ICoSS) Concept for Planetary Entry Vehicles

    Science.gov (United States)

    Kellas, Sotiris

    2016-01-01

    Results from the design, manufacturing, and testing of a lightweight Integrated Composite Stiffened Structure (ICoSS) concept, intended for multi-mission planetary entry vehicles are presented. Tests from both component and full-scale tests for a typical Earth Entry Vehicle forward shell manufactured using the ICoSS concept are presented and advantages of the concept for the particular application of passive Earth Entry Vehicles over other structural concepts are discussed.

  17. CLaSS Computer Literacy Software: From Design to Implementation - A Three Year Student Evaluation

    OpenAIRE

    Ian Cole

    2006-01-01

    Both computer literacy and information retrieval techniques are required to undertake studies in higher education in the United Kingdom. This paper considers the research, development and the 3-year student evaluation of a piece of learning technology in computer and information literacy (CLaSS software). Students completed a questionnaire to examine their own assessment of knowledge and competence in computer and information literacy and based on this assessment CLaSS software was created to...

  18. Novel immunocytokine IL12-SS1 (Fv) inhibits mesothelioma tumor growth in nude mice.

    Science.gov (United States)

    Kim, Heungnam; Gao, Wei; Ho, Mitchell

    2013-01-01

    Mesothelin is a glycosylphosphatidylinositol-anchored glycoprotein that is highly expressed on the cell surface of malignant mesothelioma. Monoclonal antibodies against mesothelin are being evaluated for the treatment of mesothelioma. Immunocytokines represent a novel class of armed antibodies. To provide an alternative approach to current mesothelin-targeted antibody therapies, we have developed a novel immunocytokine based on interleukin-12 (IL12) and the SS1 Fv specific for mesothelin. IL12 possesses potent anti-tumor activity in a wide variety of solid tumors. The newly-developed recombinant immunocytokine, IL12-SS1 (Fv), was produced in insect cells using a baculovirus-insect cell expression system. The SS1 single-chain Fv was fused to the C terminus of the p35 subunit of IL12 through a short linker (GSADGG). The single-chain IL12-SS1 (Fv) immunocytokine bound native mesothelin proteins on malignant mesothelioma (NCI-H226) and ovarian (OVCAR-3) cells as well as recombinant mesothelin on A431/H9 cells. The immunocytokine retained sufficient bioactivity of IL12 and significantly inhibited human malignant mesothelioma (NCI-H226) grown in the peritoneal cavity of nude mice and showed comparable anti-tumor activity to that of the SS1P immunotoxin. IL12-SS1 (Fv) is the first reported immunocytokine to mesothelin-positive tumors and may be an attractive addition to mesothelin-targeted cancer therapies. PMID:24260587

  19. Novel immunocytokine IL12-SS1 (Fv inhibits mesothelioma tumor growth in nude mice.

    Directory of Open Access Journals (Sweden)

    Heungnam Kim

    Full Text Available Mesothelin is a glycosylphosphatidylinositol-anchored glycoprotein that is highly expressed on the cell surface of malignant mesothelioma. Monoclonal antibodies against mesothelin are being evaluated for the treatment of mesothelioma. Immunocytokines represent a novel class of armed antibodies. To provide an alternative approach to current mesothelin-targeted antibody therapies, we have developed a novel immunocytokine based on interleukin-12 (IL12 and the SS1 Fv specific for mesothelin. IL12 possesses potent anti-tumor activity in a wide variety of solid tumors. The newly-developed recombinant immunocytokine, IL12-SS1 (Fv, was produced in insect cells using a baculovirus-insect cell expression system. The SS1 single-chain Fv was fused to the C terminus of the p35 subunit of IL12 through a short linker (GSADGG. The single-chain IL12-SS1 (Fv immunocytokine bound native mesothelin proteins on malignant mesothelioma (NCI-H226 and ovarian (OVCAR-3 cells as well as recombinant mesothelin on A431/H9 cells. The immunocytokine retained sufficient bioactivity of IL12 and significantly inhibited human malignant mesothelioma (NCI-H226 grown in the peritoneal cavity of nude mice and showed comparable anti-tumor activity to that of the SS1P immunotoxin. IL12-SS1 (Fv is the first reported immunocytokine to mesothelin-positive tumors and may be an attractive addition to mesothelin-targeted cancer therapies.

  20. Toxicity of Neurons Treated with Herbicides and Neuroprotection by Mitochondria-Targeted Antioxidant SS31

    Directory of Open Access Journals (Sweden)

    P. Hemachandra Reddy

    2011-01-01

    Full Text Available The purpose of this study was to determine the neurotoxicity of two commonly used herbicides: picloram and triclopyr and the neuroprotective effects of the mitochondria-targeted antioxidant, SS31. Using mouse neuroblastoma (N2a cells and primary neurons from C57BL/6 mice, we investigated the toxicity of these herbicides, and protective effects of SS1 peptide against picloram and triclopyr toxicity. We measured total RNA content, cell viability and mRNA expression of peroxiredoxins, neuroprotective genes, mitochondrial- encoded electron transport chain (ETC genes in N2a cells treated with herbicides and SS31. Using primary neurons from C57BL/6 mice, neuronal survival was studied in neurons treated with herbicides, in neurons pretreated with SS31 plus treated with herbicides, neurons treated with SS31 alone, and untreated neurons. Significantly decreased total RNA content, and cell viability in N2a cells treated with picloram and triclopyr were found compared to untreated N2a cells. Decreased mRNA expression of neuroprotective genes, and ETC genes in cells treated with herbicides was found compared to untreated cells. Decreased mRNA expression of peroxiredoxins 1–6 in N2a cells treated with picloram was found, suggesting that picloram affects the antioxidant enzymes in N2a cells. Immunofluorescence analysis of primary neurons revealed that decreased neuronal branching and degenerating neurons in neurons treated with picloram and triclopyr. However, neurons pretreated with SS31 prevented degenerative process caused by herbicides. Based on these results, we propose that herbicides—picloram and triclopyr appear to damage neurons, and the SS31 peptide appears to protect neurons from herbicide toxicity.

  1. Toxicity of neurons treated with herbicides and neuroprotection by mitochondria-targeted antioxidant SS31.

    Science.gov (United States)

    Reddy, Tejaswini P; Manczak, Maria; Calkins, Marcus J; Mao, Peizhong; Reddy, Arubala P; Shirendeb, Ulziibat; Park, Byung; Reddy, P Hemachandra

    2011-01-01

    The purpose of this study was to determine the neurotoxicity of two commonly used herbicides: picloram and triclopyr and the neuroprotective effects of the mitochondria-targeted antioxidant, SS31. Using mouse neuroblastoma (N2a) cells and primary neurons from C57BL/6 mice, we investigated the toxicity of these herbicides, and protective effects of SS1 peptide against picloram and triclopyr toxicity. We measured total RNA content, cell viability and mRNA expression of peroxiredoxins, neuroprotective genes, mitochondrial-encoded electron transport chain (ETC) genes in N2a cells treated with herbicides and SS31. Using primary neurons from C57BL/6 mice, neuronal survival was studied in neurons treated with herbicides, in neurons pretreated with SS31 plus treated with herbicides, neurons treated with SS31 alone, and untreated neurons. Significantly decreased total RNA content, and cell viability in N2a cells treated with picloram and triclopyr were found compared to untreated N2a cells. Decreased mRNA expression of neuroprotective genes, and ETC genes in cells treated with herbicides was found compared to untreated cells. Decreased mRNA expression of peroxiredoxins 1-6 in N2a cells treated with picloram was found, suggesting that picloram affects the antioxidant enzymes in N2a cells. Immunofluorescence analysis of primary neurons revealed that decreased neuronal branching and degenerating neurons in neurons treated with picloram and triclopyr. However, neurons pretreated with SS31 prevented degenerative process caused by herbicides. Based on these results, we propose that herbicides--picloram and triclopyr appear to damage neurons, and the SS31 peptide appears to protect neurons from herbicide toxicity. PMID:21318024

  2. Differential Role of the T6SS in Acinetobacter baumannii Virulence

    Science.gov (United States)

    Foucault-Grunenwald, Marie-Laure; Borges, Vitor; Charpentier, Xavier; Limansky, Adriana S.; Gomes, João Paulo; Viale, Alejandro M.; Salcedo, Suzana P.

    2015-01-01

    Gram-negative bacteria, such as Acinetobacter baumannii, are an increasing burden in hospitals worldwide with an alarming spread of multi-drug resistant (MDR) strains. Herein, we compared a type strain (ATCC17978), a non-clinical isolate (DSM30011) and MDR strains of A. baumannii implicated in hospital outbreaks (Ab242, Ab244 and Ab825), revealing distinct patterns of type VI secretion system (T6SS) functionality. The T6SS genomic locus is present and was actively transcribed in all of the above strains. However, only the A. baumannii DSM30011 strain was capable of killing Escherichia coli in a T6SS-dependent manner, unlike the clinical isolates, which failed to display an active T6SS in vitro. In addition, DSM30011 was able to outcompete ATCC17978 as well as Pseudomonas aeruginosa and Klebsiella pneumoniae, bacterial pathogens relevant in mixed nosocomial infections. Finally, we found that the T6SS of DSM30011 is required for host colonization of the model organism Galleria mellonella suggesting that this system could play an important role in A. baumannii virulence in a strain-specific manner. PMID:26401654

  3. Previously unknown and highly divergent ssDNA viruses populate the oceans.

    Science.gov (United States)

    Labonté, Jessica M; Suttle, Curtis A

    2013-11-01

    Single-stranded DNA (ssDNA) viruses are economically important pathogens of plants and animals, and are widespread in oceans; yet, the diversity and evolutionary relationships among marine ssDNA viruses remain largely unknown. Here we present the results from a metagenomic study of composite samples from temperate (Saanich Inlet, 11 samples; Strait of Georgia, 85 samples) and subtropical (46 samples, Gulf of Mexico) seawater. Most sequences (84%) had no evident similarity to sequenced viruses. In total, 608 putative complete genomes of ssDNA viruses were assembled, almost doubling the number of ssDNA viral genomes in databases. These comprised 129 genetically distinct groups, each represented by at least one complete genome that had no recognizable similarity to each other or to other virus sequences. Given that the seven recognized families of ssDNA viruses have considerable sequence homology within them, this suggests that many of these genetic groups may represent new viral families. Moreover, nearly 70% of the sequences were similar to one of these genomes, indicating that most of the sequences could be assigned to a genetically distinct group. Most sequences fell within 11 well-defined gene groups, each sharing a common gene. Some of these encoded putative replication and coat proteins that had similarity to sequences from viruses infecting eukaryotes, suggesting that these were likely from viruses infecting eukaryotic phytoplankton and zooplankton.

  4. Analysis of the stress-inducible transcription factor SsNAC23 in sugarcane plants

    Directory of Open Access Journals (Sweden)

    Renata Fava Ditt

    2011-08-01

    Full Text Available Stresses such as cold and drought can impair plant yield and induce a highly complex array of responses. Sugarcane (Saccharum spp. is cultivated in tropical and subtropical areas and is considered a cold-sensitive plant. We previously showed that cold stress induces the expression of several genes in in vitro sugarcane plantlets. Here we characterize one of those genes, SsNAC23, a member of the NAC family of plant-specific transcription factors, which are induced by low temperature and other stresses in several plant species. The expression of SsNAC23 was induced in sugarcane plants exposed to low temperatures (4ºC. With the aim of further understanding the regulatory network in response to stress, we used the yeast two-hybrid system to identify sugarcane proteins that interact with SsNAC23. Using SsNAC23 as bait, we screened a cDNA expression library of sugarcane plants submitted to 4ºC for 48 h. Several interacting partners were identified, including stress-related proteins, increasing our knowledge on how sugarcane plants respond to cold stress. One of these interacting partners, a thioredoxin h1, offers insights into the regulation of SsNAC23 activity.

  5. Differential Role of the T6SS in Acinetobacter baumannii Virulence.

    Directory of Open Access Journals (Sweden)

    Guillermo D Repizo

    Full Text Available Gram-negative bacteria, such as Acinetobacter baumannii, are an increasing burden in hospitals worldwide with an alarming spread of multi-drug resistant (MDR strains. Herein, we compared a type strain (ATCC17978, a non-clinical isolate (DSM30011 and MDR strains of A. baumannii implicated in hospital outbreaks (Ab242, Ab244 and Ab825, revealing distinct patterns of type VI secretion system (T6SS functionality. The T6SS genomic locus is present and was actively transcribed in all of the above strains. However, only the A. baumannii DSM30011 strain was capable of killing Escherichia coli in a T6SS-dependent manner, unlike the clinical isolates, which failed to display an active T6SS in vitro. In addition, DSM30011 was able to outcompete ATCC17978 as well as Pseudomonas aeruginosa and Klebsiella pneumoniae, bacterial pathogens relevant in mixed nosocomial infections. Finally, we found that the T6SS of DSM30011 is required for host colonization of the model organism Galleria mellonella suggesting that this system could play an important role in A. baumannii virulence in a strain-specific manner.

  6. Analysis of T4SS-induced signaling by H. pylori using quantitative phosphoproteomics

    Directory of Open Access Journals (Sweden)

    Frithjof eGlowinski

    2014-07-01

    Full Text Available Helicobacter pylori is a Gram-negative bacterial pathogen colonizing the human stomach. Infection with H. pylori causes chronic inflammation of the gastric mucosa and may lead to peptic ulceration and/or gastric cancer. A major virulence determinant of H. pylori is the type IV secretion system (T4SS, which is used to inject the virulence factor CagA into the host cell, triggering a wide range of cellular signaling events. Here, we used a phosphoproteomic approach to investigate tyrosine signaling in response to host-pathogen interaction, using stable isotope labeling in cell culture (SILAC of AGS cells to obtain a differential picture between multiple infection conditions. Cells were infected with wild type H. pylori P12, a P12ΔCagA deletion mutant, and a P12ΔT4SS deletion mutant to compare signaling changes over time and in the absence of CagA or the T4SS. Tryptic peptides were enriched for tyrosine (Tyr phosphopeptides and analysed by nano-LC-Orbitrap MS. In total, 58 different phosphosites were found to be regulated following infection. The majority of phosphosites identified were kinases of the MAPK familiy. CagA and the T4SS were found to be key regulators of Tyr phosphosites. Our findings indicate that CagA primarily induces activation of ERK1 and integrin linked factors, whereas the T4SS primarily modulates JNK and p38 activation.

  7. Echinococcus granulosus sensu stricto (s.s.) from the critically endangered antelope Addax nasomaculatus in Tunisia.

    Science.gov (United States)

    Boufana, Belgees; Saïd, Yousra; Dhibi, Mokhtar; Craig, Philip S; Lahmar, Samia

    2015-12-01

    Echinococcus granulosus sensu lato (s.l.) is a zoonotic disease highly endemic in Tunisia. Canids including stray and semi-stray dogs, jackals and foxes are known as definitive hosts and a wide range of ungulates have been shown to harbour the metacestode hydatid stage and may serve as intermediate hosts. Fertile hydatid cysts of Echinococcus equinus and E. granulosus sensu stricto (s.s.) were recently molecularly identified for the first time from Tunisian donkeys. E. granulosus (s.s.) was also identified from wild boars in Tunisia. Here we report the confirmation of hydatid cysts caused by E. granulosus (s.s.) in the critically endangered antelope, Addax nasomaculatus in Tunisia. DNA-based molecular analysis revealed that A.nasomaculatus was infected with E. granulosus (s.s.) which had a 100% identity with the main globally distributed E. granulosus (s.s.) (EgTu01) haplotype. Cysts of Taenia hydatigena (n=33) were also observed on the liver and in the body cavity. Due to their endangered status and their relatively small numbers, it is unlikely that hydatid infection of A. nasomaculatus will form a major contribution to the epidemiology and transmission of E. granulosus in Tunisia, but infection may result in pathology, morbidity and early mortality, and may still play a role in the perpetuation of the parasite in wildlife cycles.

  8. On fitting the full spectrum of luminous red galaxies by using ULySS and STARLIGHT

    International Nuclear Information System (INIS)

    We select a sample of quiescent luminous red galaxies (LRGs) from the Sloan Digital Sky Survey Data Release 7 with a high signal-to-noise ratio (S/N) to study the consistency of fitting the full spectrum by using different packages, mainly, ULySS and STARLIGHT. The spectrum of each galaxy in the sample is fitted by the full spectrum fitting packages ULySS and STARLIGHT. We find: (1) for spectra with higher S/Ns, the ages of stellar populations obtained from ULySS are slightly older than those from STARLIGHT, and metallicities derived from ULySS are slightly richer than those from STARLIGHT. In general, both packages can give roughly consistent fitting results. (2) For low S/N spectra, it is possible that the fitting by ULySS can become trapped at some local minimum in the parameter space during execution and thus may give unreliable results, but STARLIGHT can still give reliable results. Based on the fitting results of LRGs, we further analyze their star formation history and the relation between their age and velocity dispersion, and find that they agree well with conclusions from previous works

  9. Structure/property (constitutive and dynamic strength/damage characterization of additively manufactured 316L SS

    Directory of Open Access Journals (Sweden)

    Gray III G.T.

    2015-01-01

    Full Text Available For additive manufacturing (AM, the certification and qualification paradigm needs to evolve as there exists no “ASTM-type” additive manufacturing certified process or AM-material produced specifications. Accordingly, utilization of AM materials to meet engineering applications requires quantification of the constitutive properties of these evolving materials in comparison to conventionally-manufactured metals and alloys. Cylinders of 316L SS were produced using a LENS MR-7 laser additive manufacturing system from Optomec (Albuquerque, NM equipped with a 1kW Yb-fiber laser. The microstructure of the AM-316L SS is detailed in both the as-built condition and following heat-treatments designed to obtain full recrystallization. The constitutive behavior as a function of strain rate and temperature is presented and compared to that of nominal annealed wrought 316L SS plate. The dynamic damage evolution and failure response of all three materials was probed using flyer-plate impact driven spallation experiments at a peak stress of 4.5 GPa to examine incipient spallation response. The spall strength of AM-produced 316L SS was found to be very similar for the peak shock stress studied to that of annealed wrought or AM-316L SS following recrystallization. The damage evolution as a function of microstructure was characterized using optical metallography.

  10. SS400钢卷冷弯不合格原因分析%Analysis on the Reason Causing Unqualified Cold Bending of SS400 Steel Coil

    Institute of Scientific and Technical Information of China (English)

    胡亚平

    2011-01-01

    通过宏观检验、力学性能测试、化学成分分析和金相检验对SS400钢卷冷弯不合格的原因进行了分析。结果表明:钢板中氧化铝夹杂偏多是冷弯试样出现裂纹的主要原因,同时提出了减少冷弯裂纹出现的措施。%By means of macroscopic examination, mechanical property testing, chemical composition analysis and metallographic examination, the reason causing unqualified cold bending property of SS400 steel coil was analyzed. The results showed that the main reason was higher content of alumina inclusion in the cold bending sample. And the measures to reduce the cracks of cold bending were given out.

  11. 3D-hahmon luonti ja ohjaaminen Cave-virtuaaliympäristössä

    OpenAIRE

    Orava, Peetu

    2011-01-01

    ssä työssä on perehdytty 3D-hahmon luonnin eri vaiheisiin, joihin kuuluu suunnittelu Photoshopissa, mallinnus 3ds Maxissa, yksityiskohtien ja tekstuurien luonti ZBrushissa. Mallinnetulle hahmolle on myös luotu Biped-luujärjestelmä 3ds Maxissa Skin-muokkaimen kanssa. Tämän jälkeen valmis hahmo on viety Virtools-ohjelmaan ja siinä luotu hahmolle kontrollit peliohjaimelle, jotta sitä voi ohjata Cave-virtuaaliympäristössä käyttäen valmiita animaatioita. Samalla käydään läpi työnkulun eri v...

  12. Asymmetric PCR method in generation of HBV ssDNA for pyrosequencing

    Institute of Scientific and Technical Information of China (English)

    Nian-cai Peng; Chun-lin Wang; Li-li Zhang; Mao-li Lu; Zhen-xi Zhang

    2009-01-01

    Objective To explore the optimal primer ratio and concentration of asymmetric polymerase chain reaction (A-PCR) in producing hepatitis B virus (HBV) single-stranded DNA (ssDNA) for pyrosequencing. Methods A-PCR was carried out to generate HBV ssDNA with forward to reverse primers of different ratios (50 : 1, 100 : 1) and concentrations (13. 0 pmol/25μL and 0.14 pmol/25μL, 19. 5 pmol/25μL and 0. 21 pmol/25μL), and the product yield and quality were compared respectively. Results The forward to reverse primer ratio of 50 : 1 provided better yield and concentration of 19. 5 pmol/25μL and 0. 21 pmol//25μL generated a clearer band. Conclusion A simple and feasible method to produce HBV ssDNA for pyrosequencing in batch is established.

  13. Henkilöstön motivoinnin ja sitouttamisen keinoja esimiestyössä

    OpenAIRE

    Lahtonen-Pääkkönen, Katja; Salohalme, Tommi

    2015-01-01

    ssä opinnäytetyössä on tarkasteltu keinoja henkilöstön motivoimiseen ja sitouttamiseen esimiestyössä. Opinnäytetyön tärkeimpänä tavoitteena oli löytää konkreettisia keinoja ja menetelmiä kollegoille sekä yrityksien henkilöstölle käytettäväksi henkilöstön motivoimiseksi ja sitouttamiseksi. Tämän lisäksi tarkoituksena oli kehittyä itse ammatillisesti ihmisten johtajana. Käsillä oleva opinnäytetyö rakentuu teoreettisista sekä empiirisista luvuista. Tutkimuksen teoreettinen viitekehys koo...

  14. Harjoituskäytössä olevien nestesammuttimien huolto-ohjeet

    OpenAIRE

    Nummela, Juuso

    2014-01-01

    Tämän opinnäytetyön lähtökohtana oli Turvallisuus ja kemikaalivirastolle, Tukesille, tulleet toistuvat kyselyt kuinka harjoituskäytössä olevien nestetäytteisten sammuttimien huolto tulisi toteuttaa. Työ toteutettiin perehtymällä eri toimijoiden huoltokäytäntöihin harjoituskäytössä olevien sammuttimien osalta, sekä selvitin kuinka lainsäädäntö ja muu normisto ohjeistavat harjoituskäytössä olevien sammuttimien huoltoa. Huoltokäytäntöjä on selvitetty haastattelemalla eri toimijoita joilla o...

  15. Nucleation and growth microstructural study of ti films on 304 SS substrates

    Directory of Open Access Journals (Sweden)

    Rogério de Almeida Vieira

    2004-09-01

    Full Text Available Coating of steel surfaces with titanium films has been studied with the objective to protect them against corrosion, and to create an intermediate film for CVD diamond and TiN film deposition. In this work, the nucleation, growth mechanisms and microstructural formation of the titanium films deposited on 304 stainless steel (304 SS substrate are presented and discussed. The titanium films of variable thickness were obtained by vapour phase deposition produced by electron beam. The surfaces of these samples were observed by scanning electron microscopy. The cross sections of these samples were observed by using an atomic force microscope. The Ti film-304 SS interfaces were analyzed by X-ray diffraction. The results showed that titanium films have a columnar growth. The Ti film-304 SS interface had a residual compression stress at room temperature due to the inter-diffusion process.

  16. TIMES-SS - A mechanistic evaluation of an external validation study using reaction chemistry principles

    DEFF Research Database (Denmark)

    Roberts, David W.; Patlewicz, Grace; Dimitrov, Sabcho D.;

    2007-01-01

    -skin metabolism relationships through a number of transformations, some of which are underpinned by mechanistic three-dimensional quantitative structure-activity relationships. Here, we describe an external validation exercise that was recently carried out. As part of this exercise, data were generated for 40 new...... chemicals in the murine local lymph node assay (LLNA) and then compared with predictions made by TIMES-SS. The results were promising with an overall good concordance (83%) between experimental and predicted values. The LLNA results were evaluated with respect to reaction chemistry principles...... for sensitization. Additional testing on a further four chemicals was carried out to explore some of the specific reaction chemistry findings in more detail. Improvements for TIMES-SS, where appropriate, were put forward together with proposals for further research work. TIMES-SS is a promising tool to aid...

  17. Resistance of superhydrophobic and oleophobic surfaces to varied temperature applications on 316L SS

    Science.gov (United States)

    Shams, Hamza; Basit, Kanza; Saleem, Sajid; Siddiqui, Bilal A.

    316L SS also called Marine Stainless Steel is an important material for structural and marine applications. When superhydrophobic and oleophobic coatings are applied on 316L SS it shows significant resistance to wear and corrosion. This paper aims to validate the coatings manufacturer's information on optimal temperature range and test the viability of coating against multiple oil based cleaning agents. 316L SS was coated with multiple superhydrophic and oleohobic coatings and observed under SEM for validity of adhesion and thickness and then scanned under FFM to validate the tribological information. The samples were then dipped into multiple cleaning agents maintained at the range of operating temperatures specified by the manufacturer. Coating was observed for deterioration over a fixed time intervals through SEM and FFM. A comparison was drawn to validate the most critical cleaning agent and the most critical temperature at which the coating fails to leave the base substrate exposed to the environment.

  18. Development of Ss-NIE-1 recombinant antigen based assays for immunodiagnosis of strongyloidiasis.

    Science.gov (United States)

    Rascoe, Lisa N; Price, Courtney; Shin, Sun Hee; McAuliffe, Isabel; Priest, Jeffrey W; Handali, Sukwan

    2015-04-01

    Strongyloides stercoralis is a widely distributed parasite that infects 30 to 100 million people worldwide. In the United States strongyloidiasis is recognized as an important infection in immigrants and refugees. Public health and commercial reference laboratories need a simple and reliable method for diagnosis of strongyloidiasis to identify and treat cases and to prevent transmission. The recognized laboratory test of choice for diagnosis of strongyloidiasis is detection of disease specific antibodies, most commonly using a crude parasite extract for detection of IgG antibodies. Recently, a luciferase tagged recombinant protein of S. stercoralis, Ss-NIE-1, has been used in a luciferase immunoprecipitation system (LIPS) to detect IgG and IgG4 specific antibodies. To promote wider adoption of immunoassays for strongyloidiasis, we used the Ss-NIE-1 recombinant antigen without the luciferase tag and developed ELISA and fluorescent bead (Luminex) assays to detect S. stercoralis specific IgG4. We evaluated the assays using well-characterized sera from persons with or without presumed strongyloidiasis. The sensitivity and specificity of Ss-NIE-1 IgG4 ELISA were 95% and 93%, respectively. For the IgG4 Luminex assay, the sensitivity and specificity were 93% and 95%, respectively. Specific IgG4 antibody decreased after treatment in a manner that was similar to the decrease of specific IgG measured in the crude IgG ELISA. The sensitivities of the Ss-NIE-1 IgG4 ELISA and Luminex assays were comparable to the crude IgG ELISA but with improved specificities. However, the Ss-NIE-1 based assays are not dependent on native parasite materials and can be performed using widely available laboratory equipment. In conclusion, these newly developed Ss-NIE-1 based immunoassays can be readily adopted by public health and commercial reference laboratories for routine screening and clinical diagnosis of S. stercoralis infection in refugees and immigrants in the United States.

  19. Binding hot-spots in an antibody-ssDNA interface: a molecular dynamics study.

    Science.gov (United States)

    Wang, Yeng-Tseng; Lee, Wen-Jay

    2012-10-30

    Simulating antigen-antibody interactions is essential for elucidating antigen-antibody mechanics. Proteins interactions are vital for elucidating antibody-ssDNA associations in immunology. Therefore, this study investigated the dissociation of the human systemic lupus erythematosus antibody-ssDNA complex structure. Dissociation (i.e. the distance between the center of mass of the ssDNA and the antibody) is also studied using the potential of mean force calculations based on molecular dynamics and the explicit water model. The MM-PBSA method is also used to prove our dissociation simulations. With 605 nanosecond molecular dynamics simulations, the results indicate that the 8 residues (i.e. Gly44 (HCDR2), Asn54 (HCDR2), Arg98 (HCDR3), Tyr100 (HCDR3), Asp101 (HCDR3), Tyr32 (LCDR1), Tyr49 (LCDR2) and Asn50 (LCDR2)), and the five inter-protein molecular hydrogen bonds may profoundly impact the antibody-ssDNA interaction, a finding which may be useful for protein engineering of this antibody-ssDNA structure. Experimental binding affinity of this antibody-ssDNA complex equals 7.00 kcal mol(-1). Our dissociation binding affinity is 7.96 ± 0.33 kcal mol(-1) and MM-PBSA binding affinity is 9.12 ± 1.65 kcal mol(-1), which is close to the experimental value. Additionally, the 8 residues Gly44 (HCDR2), Asn54 (HCDR2), Arg98 (HCDR3), Tyr100 (HCDR3), Asp101 (HCDR3), Tyr32 (LCDR1), Tyr49 (LCDR2) and Asn50 (LCDR2) may play a more significant role in developing bioactive antibody analogues. PMID:23079742

  20. Serum testosterone levels of HbSS (sickle cell disease male subjects in Lagos, Nigeria

    Directory of Open Access Journals (Sweden)

    Adediran Adewumi

    2011-08-01

    Full Text Available Abstract Background Infertility is a major problem in sickle cell disease patients, especially in males. In addition to low serum testosterone, other abnormalities involving the accessory sex organs, such as the seminal vesicles and the prostate gland, as well as marked decrease in ejaculate volume may be observed in male HbSS patients. Hence, the need to study the role of sex hormones as a cause of infertility in male HbSS patients. Methods An unmatched case-control study was performed using seventy-five consenting subjects from Lagos University Teaching Hospital. These included 47 patients with haemoglobin phenotype SS from the Sickle cell clinic and 28 volunteered medical students and members of staff with haemoglobin phenotype AA. Demographic data were obtained using a self-administered questionnaire. A total of 5 mls of blood was collected from each subject between 9.00 am & 11.am, and assayed for serum testosterone concentration. Results The concentrations of serum testosterone in HbSS patients ranged from 0.2 to 4.3 ng/ml with a mean of 1.28 ± 0.72 ng/ml whilst the values in HbAA controls ranged from 1.2 to 6.9 ng/ml with a mean of 2.63 ± 1.04 ng/ml. Seven (25.0% of the 28 controls had serum testosterone concentration lower than the quoted reference (normal range whereas 44 (93.6% of the 47 HbSS subjects had serum testosterone concentration lower than the reference range. Conclusion Overall, subjects with HbSS have significantly lower mean serum testosterone than HbAA controls.

  1. Cyclic behavior of SS 316 LN with respect to thermal ratcheting of main vessel for SFR

    International Nuclear Information System (INIS)

    One of the innovative features which are considered for the future Sodium cooled Fast breeder Reactor (SFR) in India is the elimination of main vessel cooling circuit. This would bring out significant economic by saving the cost of entire cooling circuit and also by reducing the main vessel diameter. However, the challenging aspect to be addressed is investigation of thermal ratcheting failure near sodium free level. The sodium free level will vary during the various operating conditions depending up on hot and cold pool temperatures. The thermal fluctuations cause high cycle deformation controlled fatigue damage. These high cycle temperature oscillations cause high cycle deformation controlled fatigue on metal surface. Thermal ratcheting of main vessel enhanced by creep effect which cause radial deformations near the sodium free level during Safety Graded Decay Heat Removal (SGDHR). The strain accumulated due to thermal ratcheting can also cause reduction in buckling strength of main vessel. The accumulated deformations can cause concern for the free movement of in-service inspection equipment. Preliminary assessment indicates that there is a need to develop special SS 316 LN with higher nitrogen content and subsequently to demonstrate that the vessel made of this material can provide a sufficient safety margin against ratcheting. This exercise calls for carrying out many simulated experiments, particularly to predict thermal ratcheting. Some of the recent bi-axial tension-torsion experiments to simulate ratcheting for SS316LN and enhanced nitrogen steel (SS316EN) found that the axial strains predicted by enhanced nitrogen SS316EN steel were much higher than the strains predicted for SS316LN steel for the same set of parameters. Hence it is worthwhile to carryout investigations to predict the cyclic behavior of SS 316LN and enhanced nitrogen steel with respect to thermal ratcheting. Some experiments have been conducted for the SFR scale down model of main vessel

  2. A theoretical investigation on anomalous switching of single-stranded deoxyribonucleic acid (ssDNA) monolayers by water vapor

    Institute of Scientific and Technical Information of China (English)

    赵新军; 高志福; 蒋中英

    2015-01-01

    In this paper, we use a molecular theory to study the anomalous switching of ssDNA monolayers. Here, both ssDNA–water and water–water hydrogen bonds and their explicit coupling to the ssDNA conformations are considered. We find that hydrogen bonding becomes a key element in inducing the anomalous switching of ssDNA monolayers. This finding accords well with the experimental observations. Based on our theoretical model, we predict that the anomalous switching induced by water vapor will be applicable to a wide range of hydrogen bonds polymers, and ssDNA–water hydrogen bonds and water–water hydrogen bonds hybridization will lead to the hydrogen-bond network formation of 3D ssDNA monolayers.

  3. Exploring optimization parameters to increase ssDNA recombineering in Lactococcus lactis and Lactobacillus reuteri

    OpenAIRE

    van Pijkeren, Jan-Peter; Neoh, Kar Mun; Sirias, Denise; Findley, Anthony S.; Britton, Robert A.

    2012-01-01

    Single-stranded DNA (ssDNA) recombineering is a technology which is used to make subtle changes in the chromosome of several bacterial genera. Cells which express a single-stranded DNA binding protein (RecT or Bet) are transformed with an oligonucleotide which is incorporated via an annealing and replication-dependent mechanism. By in silico analysis we identified ssDNA binding protein homologs in the genus Lactobacillus and Lactococcus lactis. To assess whether we could further improve the r...

  4. Kehitysvammaisuus suomalaisessa elokuvataiteessa 1920-luvulta vuoteen 2010 : Yhteiskunnallisia merkityksiä etsimässä

    OpenAIRE

    Markus, Helena

    2011-01-01

    Tutkimustyöni lähtökohtana on sosionomin tehtäväkentässä kulttuurillisuuden vahvistaminen. Kehitysvammaisuus suomalaisessa elokuvataiteessa – yhteiskunnallisia merkityksiä etsimässä, on lyhyt katsaus kotimaisesta elokuvasta 1920-luvulta nykypäivään. Tavoitteenani on etsiä vuosikymmenittäin elokuvia, joissa on kehitysvammaiseksi henkilöksi luonnehdittava hahmo roolisuorituksena. Löytämistäni elokuvista olen analysoinut sosiaalipoliittisten tavoitteiden, asenteiden ja arvojen näkymistä kullakin...

  5. Suklaajälkiruoat ja niiden valmistamisen haasteet ammattikeittiössä

    OpenAIRE

    Lappalainen, Arto

    2016-01-01

    Tämä opinnäytetyö on osa palvelujen tuottamisen ja johtamisen koulutusohjelmaa. Opinnäytetyön aiheena on suklaajälkiruuat ja niiden valmistamisen haasteet ammattikeittiössä. Opinnäytetyön teoriaosuudessa käsitellään suklaan historiaa, lajikkeita, tuottajamaita, valmistusmenetelmiä, suklaan laatuja, tärkeimpiä valmistajia sekä suklaan käyttöä ja tekniikoita ammattikeittiössä. Tutkimuksessa selvitettiin yleisimpiä suklaajälkiruokia ja niiden valmistuksessa olevia haasteita ammattikeitti...

  6. Taekwon-do : Sosiaalinen tuki vanhempien kasvatustehtävässä

    OpenAIRE

    Kangasniemi, Tanja

    2012-01-01

    Tämän opinnäytetyön päätarkoituksena on selvittää Taekwon-Don antamaa kasvatuskumppanuutta ja sosiaalista tukea vanhemmille. Minkälaista sosiaalista tukea Taekwon-Don pääperiaatteet ja filosofia voivat tuoda vanhempien kasvatustehtävään? Taekwon-Do filosofia pohjautuu lajin kehittäjän kenraali Choi Hong Hi:n kirjoittamiin periaatteisiin, elämänfilosofiaan ja protokollaan. Pääperiaatteet ovat: rehellisyys, kohteliaisuus, uutteruus, itsehillintä ja lannistumaton henki. Tässä työssä Taekwon-...

  7. Ylisukupolvisuus toimeentulotukityössä : sosiaaliohjaajien työskentely perheiden kanssa

    OpenAIRE

    Meling, Maiju; Strellman, Merja

    2015-01-01

    TIIVISTELMÄ Meling, Maiju & Strellman, Merja. Ylisukupolvisuus toimeentulotukityössä. Sosiaaliohjaajien työskentely perheiden kanssa. Diak, Helsinki, kevät 2015, 76 s., 4 liitettä. Diakonia-ammattikorkeakoulu, Sosiaalialan koulutusohjelma, Päihteet ja syrjäytyminen, sosionomi (YAMK). Opinnäytetyön tavoitteena on kartoittaa toimeentulotukiasiakkuuden ylisukupolvisuutta ilmiönä ja aikuissosiaalityön asiakasperheiden kanssa tehtävää työtä sosiaaliohjauksen näkökulmasta. Opinnäytetyöss...

  8. Perehdyttäminen Lastenlinnan video-EEG-yksikössä

    OpenAIRE

    Junnonen, Satu

    2009-01-01

    TIIVISTELMÄ Junnonen, Satu. Perehdyttäminen Lastenlinnan video-EEG-yksikössä. Helsinki, syksy 2009, 77 s., 2 liitettä. Diakonia-ammattikorkeakoulu, Diak Etelä, Helsinki. Hoitotyön koulutusohjelma, Hoitotyön suuntautumisvaihtoehto, muuntokoulutus, sairaanhoitaja (AMK). Opinnäytetyöni on produktio. Sen tarkoituksena oli laatia kirjallinen perehdytyskansio epilepsiaosaston hoitajille video-EEG-yksikössä työskentelystä. Video-EEG ja epilepsiaosasto LL11 kuuluvat molemmat Helsingin Las...

  9. Toimintaterapia-arviointi osana kuntoutuksen suunnittelua kuntoutustyöryhmässä

    OpenAIRE

    Rantanen, Sanna; Pehkonen, Riitta

    2011-01-01

    ssä opinnäytetyössä tavoitteena oli löytää sopivia,näyttöön perustuvia ja moniammatillisesti hyödynnettäviä toimintaterapian arviointimenetelmiä Pohjois-Karjalan sairaanhoito- ja sosiaalipalvelujen kuntayhtymän (PKSSK) aikuispsykiatrian poliklinikan kuntoutustyöryhmän käyttöön. Opinnäytetyön tutkimus oli kvalitatiivinen ja se toteutettiin ryhmämuotoisella teemahaastattelulla, sekä kirjallisuuskatsauksella. Kuntoutustyöryhmän haastattelussa selvitettiin PKSSK:n aikuispsykiatrian poliklinikan...

  10. Tutkimus työturvallisuudesta KerabitPro Oy:ssä

    OpenAIRE

    Savinainen, Kimmo

    2013-01-01

    Opinnäytetyössä tutkittiin työturvallisuuden tasoa erityisesti yrityksen pää-urakoimilla työmailla. Tutkimus tehtiin eri puolella Suomea 5:llä eri alueella. Turvallisuustason mittaukseen käytettiin talonrakennus eli TR-mittausta, koska se on yrityksessä muutoinkin käytössä. Tämä mahdollisti tulosten vertailun yrityksen sisäisiin mittauksiin. Tutkimus tehtiin loka-marraskuun 2012 aikana ja siinä käytiin läpi yhteensä 25 työmaata. Kokonaisuutena työturvallisuuden taso oli hyvä, mutta poikk...

  11. Asymmetric PCR method in generation of HBV ssDNA for pyrosequencing

    Institute of Scientific and Technical Information of China (English)

    2009-01-01

    Objective To explore the optimal primer ratio and concentration of asymmetric polymerase chain reaction (A-PCR) in producing hepatitis B virus (HBV) single-stranded DNA (ssDNA) for pyrosequencing. Methods A-PCR was carried out to generate HBV ssDNA with forward to reverse primers of different ratios (50∶1, 100∶1) and concentrations (13.0 pmol/25μL and 0.14 pmol/25μL, 19.5 pmol/25μL and 0.21 pmol/25μL), and the product yield and quality were compared respectively. Results The forward to reverse primer ratio ...

  12. Tiimityön toimivuus päivystyshoitotyössä sairaanhoitajien kokemana

    OpenAIRE

    Lahdenperä, Ville; Märijärvi, Janne

    2012-01-01

    Lahdenperä, Ville & Märijärvi, Janne. Tiimityön toimivuus päivystyshoitotyössä sairaanhoitajien kokemana. Helsinki, Kevät 2012, 59 s, 4 liitettä. Diakonia-ammattikorkeakoulu, Diak Etelä, Helsinki. Sosiaali- ja terveysalan koulutusohjelma, hoitotyön suuntautumisvaihtoehto, sairaanhoitaja (AMK). Tämän opinnäytetyön tarkoitus on kuvata, miten Peijaksen sairaalan päivystysalueella työskentelevät sairaanhoitajat kokevat moniammatillisen tiimityön toimivan päivystyshoitotyössä. Työn tarkoit...

  13. Sosiaalisen median mahdollisuudet markkinointi- ja asiakasviestinnässä - tapaus Yritys Oy

    OpenAIRE

    Mikkonen, Kirsi

    2012-01-01

    Yritysviestinnän näkökulmasta sosiaalinen media on mielenkiintoinen sekoitus viestintää, markkinointia ja asiakaspalvelua. Tämän opinnäytetyön aiheena on sosiaalisen median hyödyntäminen yrityksen markkinointi- ja asiakasviestinnässä. Tarkoituksena oli selvittää, millaisia sosiaalisen median työkaluja toimeksiantaja Yritys Oy voisi käyttää ulkoisessa viestinnässään. Tutkimusmenetelmänä oli kirjallisuuskatsaus, johon sisällytettiin viestinnän, markkinoinnin ja sosiaalisen median kirjallisuu...

  14. Maailma kylässä 2014 -festivaali: Näytteilleasettajatutkimus

    OpenAIRE

    Stylman, Maija

    2014-01-01

    Opinnäytetyön aiheena on Maailma kylässä 2014 –festivaalin näytteilleasettajatutkimus. Määrällisen tutkimuksen tarkoituksena oli selvittää näytteilleasettajien kokemuksia ja mielipiteitä festivaalista. Tutkimuksen tavoitteena oli löytää ideoita, joilla voisi kehittää festivaalin näytteilleasettajatoimintaa seuraavana vuonna. Tutkimuskysymyksiä oli kolme: miksi näytteilleasettajat osallistuivat Maailma kylässä –festivaalille tänä vuonna, miten he arvioivat festivaalin onnistumista omasta näkök...

  15. Työssäoppimisjaksojen kehittäminen oppaiden avulla : Helsingin kaupungin liikuntaviraston liikunnanohjausosastolla

    OpenAIRE

    Hakkarainen, Jarna

    2014-01-01

    Opinnäytetyöprojektin tarkoituksena oli luoda Helsingin kaupungin liikuntaviraston liikunnanohjausosaston liikunnanohjaajien käyttöön opas työssäoppijoiden ohjaami-seen. Opas on nimensä mukaan apuväline liikunnanohjaajille opiskelijoiden ohjaami-seen. Toisena oppaana valmistui työpaikkaohjaajien sekä opiskelijoiden käyttöön Ter-vetuloa työssäoppimisjaksolle Helsingin kaupungin liikuntaviraston liikunnanohjaus-osastolle – opas. Tervetuloa – oppaan tarkoituksena on perehdyttää opiskelija liikun...

  16. Gospel-lattarit työvälineenä kirkon nuorisotyössä

    OpenAIRE

    Joutsiniemi, Johanna

    2012-01-01

    Halusin työssäni tutkia ja avata Gospel-lattareiden hyötyjä hyvinvoinnin kannalta ajateltuna kasvatustyössä. Toiminnallisen osuuden kautta halusin tarjota nuorille mahdollisuuden tutustua Gospel-lattareihin ja kokeilla niitä lyhyiden Gospel-lattarituokioiden muodossa. Tarkoitukseni oli myös madaltaa kynnystä lähteä ehkä myöhemmin harrastamaan tanssimista, niiden kohdalla joille tanssiminen ei ollut entuudestaan niin tuttua. Halusin lisäksi tuoda ideoita kirkon nuorisotyöhön tarjoten esimerkke...

  17. All-digital Carrier Frequency Tracking Method in LEO DS/SS Communication

    Institute of Scientific and Technical Information of China (English)

    XING Ke-fei; YANG Jun; ZHANG Chuan-sheng; WANG Yue-ke

    2006-01-01

    An efficient method to track the carrier frequency which is recommended to use in low earth orbit(LEO) direct-sequence spread-spectrum(DS/SS) communication system is given. The carrier frequency drifts quicker in LEO DS/SS communication system, its tracking problem becomes much more important especially in digital receiver with the limits of physical shape and power. Under the condition of coarse carrier frequency synchronization, a digital tracking method based on least square method(LSM) is proved to be a quick, simple and efficient method. Numerical results and charts are given.

  18. Truncated SSX protein suppresses synovial sarcoma cell proliferation by inhibiting the localization of SS18-SSX fusion protein.

    Directory of Open Access Journals (Sweden)

    Yasushi Yoneda

    Full Text Available Synovial sarcoma is a relatively rare high-grade soft tissue sarcoma that often develops in the limbs of young people and induces the lung and the lymph node metastasis resulting in poor prognosis. In patients with synovial sarcoma, specific chromosomal translocation of t(X; 18 (p11.2;q11.2 is observed, and SS18-SSX fusion protein expressed by this translocation is reported to be associated with pathogenesis. However, role of the fusion protein in the pathogenesis of synovial sarcoma has not yet been completely clarified. In this study, we focused on the localization patterns of SS18-SSX fusion protein. We constructed expression plasmids coding for the full length SS18-SSX, the truncated SS18 moiety (tSS18 and the truncated SSX moiety (tSSX of SS18-SSX, tagged with fluorescent proteins. These plasmids were transfected in synovial sarcoma SYO-1 cells and we observed the expression of these proteins using a fluorescence microscope. The SS18-SSX fusion protein showed a characteristic speckle pattern in the nucleus. However, when SS18-SSX was co-expressed with tSSX, localization of SS18-SSX changed from speckle patterns to the diffused pattern similar to the localization pattern of tSSX and SSX. Furthermore, cell proliferation and colony formation of synovial sarcoma SYO-1 and YaFuSS cells were suppressed by exogenous tSSX expression. Our results suggest that the characteristic speckle localization pattern of SS18-SSX is strongly involved in the tumorigenesis through the SSX moiety of the SS18-SSX fusion protein. These findings could be applied to further understand the pathogenic mechanisms, and towards the development of molecular targeting approach for synovial sarcoma.

  19. Strategia vanginvartijan työssä : Turun vankilan vanginvartijoiden näkemyksiä Rikosseuraamuslaitoksen strategiasta 2011 – 2020

    OpenAIRE

    Granith, Linda; Koskenvuori, Enni

    2013-01-01

    Opinnäytetyössä tutkitaan Rikosseuraamuslaitoksen strategian 2011 – 2020 näkymistä Turun vankilan vanginvartijoiden jokapäiväisessä työssä. Opinnäytetyössä tutkitaan Rikosseuraamuslaitoksen strategian käsittelyä ja tiedottamista työyhteisöissä sekä sitä, ovatko vanginvartijat tutustuneet strategiaan. Strategian vaikutusta vanginvartijoiden työhön käsitellään strategiaan kirjattujen arvojen sekä vanginvartijan työhön liittyvien tavoitteiden kautta. Työssä nostetaan esiin vanginvartijoiden näke...

  20. Syntheses, Properties of [Ni (SS) (NN)]-type Mixed-ligand Complexes%[Ni(NN)(SS)]混配配合物的合成与性质[Ni(NN)(SS)]混配配合物的合成与性质

    Institute of Scientific and Technical Information of China (English)

    姚天明; 谢建军; 傅成武; 施宪法

    2001-01-01

    Four new complexes of the general formula [Ni(SS)(NN)], Where SS is dddt (5, 6-dihydro-1, 4-dithiin-2, 3-dithiolate) or pddt(6, 7-dihydro-SH-1, 4-dithiepin-2, 3-dithiolate) and NN is bpy or phen were prepared. The UV/Vis. Spectra exhibit intense intramolecular ligand-to-ligand charge transfer bands ca. 600 nm. Cyclic voltammetry shows a reversible oxidation step assigned to [Ni(SS) (NN)]0= [Ni(SS)(NN)]+. When the complex [Ni(dddt)(bpy) ] was partially oxidized by [Ni(SS) (NN)]0= [Ni(SS)(NN)]+. When the complex [Ni(dddt)(bpy) ] was partially oxidized by Ⅰ2, a broad ESR signal at g = 2. 003 appeared

  1. High-frequency genome editing using ssDNA oligonucleotides with zinc-finger nucleases

    DEFF Research Database (Denmark)

    Chen, Fuqiang; Pruett-Miller, Shondra M; Huang, Yuping;

    2011-01-01

    Zinc-finger nucleases (ZFNs) have enabled highly efficient gene targeting in multiple cell types and organisms. Here we describe methods for using simple ssDNA oligonucleotides in tandem with ZFNs to efficiently produce human cell lines with three distinct genetic outcomes: (i) targeted point...

  2. Relva-SS veteranid said kokku Sinimägedes / Rita Loel

    Index Scriptorium Estoniae

    Loel, Rita, 1966-2012

    2008-01-01

    Relva-SS veteranid kogunesid Sinimägedes, et meenutada 64 aastat tagasi toimunud lahinguid. Riigikogu liige Trivimi Velliste lubas, et juba sügisel esitab IRL Riigikogus eelnõu, mis tunnustaks ka Saksa poolel sõdinud eesti mehi

  3. Why complexity matters: using ALMaSS for risk assessment of wildlife

    DEFF Research Database (Denmark)

    Topping, Christopher John

    -sink dynamics are emergent properties and do not need to be imposed.ALMaSS is one such model system and is used here to exemplify aspects of complexity in population-level risk assessment for terrestrial mammals, birds, and arthropods exposed to pesticides. Results are used to argue for greater realism...

  4. Ball Indentation Studies on the Effect of Nitrogen on the Tensile Properties of 316LN SS

    Science.gov (United States)

    Mathew, M. D.; Ganesh Kumar, J.; Ganesan, V.; Laha, K.

    2015-12-01

    Type 316L(N) stainless steel (SS) containing 0.02-0.03 wt% carbon and 0.06-0.08 wt% nitrogen is used as the major structural material for the components of fast reactors. Research is underway to improve the high-temperature mechanical properties of 316LN SS by increasing the nitrogen content in the steel above the level of 0.08 wt%. In this investigation, ball indentation (BI) technique was used to evaluate the effect of nitrogen content on the tensile properties of 316LN SS. BI tests were conducted on four different heats of 316LN SS containing 0.07, 0.11, 0.14 and 0.22 wt% nitrogen in the temperature range 300-923 K. The tensile properties such as yield strength and ultimate tensile strength increased with increase in nitrogen content at all the investigated temperatures. These results were consistent with the corresponding uniaxial tensile test results. These studies showed that BI technique can be used to optimize the chemical composition during alloy development by evaluating tensile properties with minimum volume of material.

  5. Multifunctional DNA-based biomemory device consisting of ssDNA/Cu heterolayers.

    Science.gov (United States)

    Lee, Taek; El-Said, Waleed Ahmed; Min, Junhong; Choi, Jeong-Woo

    2011-01-15

    In the present study, we developed a novel DNA-based biomemory device that was comprised of ssDNA/Cu heterolayers on Au electrodes. As a conducting material, a thiol-modified single strand DNA (26 bp) was designed and immobilized on the Au electrode without the need for any linker material. Cu(2+) ions, which acted as the active site, were then chemically absorbed on the external structure of ssDNA through electrostatic interactions. The presence of the fabricated ssDNA/Cu heterolayer was confirmed by surface plasmon resonance (SPR) spectroscopy and Raman spectroscopy. Cyclic voltammetry experiments were carried out to investigate the redox properties of ssDNA/Cu hybrids to obtain the oxidation and reduction potential. Based on measured oxidation and reduction potential, a ROM-type, 3-state type, and WORM type DNA memory functions were demonstrated by chronoamperometry (CA) and open circuit potential amperometry (OCPA). This proposed device acts and operates the memory function very well. In the near future, DNA based biomemory device in this study could provide the alternative to the inorganic electronic device when molecular scaled immobilization control and signal measurement are achieved. PMID:21051218

  6. Evolving radio structure of the binary star SS433 at a resolution of 15 marc s

    International Nuclear Information System (INIS)

    A comprehensive series of VLBI (very-long-baseline interferometry) observations of SS433, carried out over an 11-day period on the European VLBI Network at 5 GHz, reveals the motion and evolution of a series of faint 'blobs' ejected from the system. These blobs are observed to brighten at a substantial distance from the binary star. (author)

  7. Screening and Identification of ssDNA Aptamer for Human GP73

    Directory of Open Access Journals (Sweden)

    Jingchun Du

    2015-01-01

    Full Text Available As one tumor marker of HCC, Golgi Protein 73 (GP73 is given more promise in the early diagnosis of HCC, and aptamers have been developed to compete with antibodies as biorecognition probes in different detection system. In this study, we utilized GP73 to screen specific ssDNA aptamers by SELEX technique. First, GP73 proteins were expressed and purified by prokaryotic expression system and Nickle ion affinity chromatography, respectively. At the same time, the immunogenicity of purified GP73 was confirmed by Western blotting. The enriched ssDNA library with high binding capacity for GP73 was obtained after ten rounds of SELEX. Then, thirty ssDNA aptamers were sequenced, in which two ssDNA aptamers with identical DNA sequence were confirmed, based on the alignment results, and designated as A10-2. Furthermore, the specific antibody could block the binding of A10-2 to GP73, and the specific binding of A10-2 to GP73 was also supported by the observation that several tumor cell lines exhibited variable expression level of GP73. Significantly, the identified aptamer A10-2 could distinguish normal and cancerous liver tissues. So, our results indicate that the aptamer A10-2 might be developed into one molecular probe to detect HCC from normal liver specimens.

  8. Optimal continuous order quantity (s,S) policies; the 45-degrees algorithm

    NARCIS (Netherlands)

    E.M. Bazsa-Oldenkamp; P. den Iseger

    2002-01-01

    textabstractThe most recent optimization algorithm for (s,S) order policies with continuous demand was developed by Federgruen and Zipkin (1985). This was also the first efficient algorithm, which uses policy iteration instead of discretization. Zheng and Federgruen (1991) developed an even more eff

  9. PU/SS Eutectic Assessment In 9975 Packagings In A Storage Facility During Extended Fire

    International Nuclear Information System (INIS)

    In a radioactive material (RAM) packaging, the formation of eutectic at the Pu/SS (plutonium/stainless steel) interface is a serious concern and must be avoided to prevent of leakage of fissile material to the environment. The eutectic temperature for the Pu/SS is rather low (410 C) and could seriously impact the structural integrity of the containment vessel under accident conditions involving fire. The 9975 packaging is used for long term storage of Pu bearing materials in the DOE complex where the Pu comes in contact with the stainless steel containment vessel. Due to the serious consequences of the containment breach at the eutectic site, the Pu/SS interface temperature is kept well below the eutectic formation temperature of 410 C. This paper discusses the thermal models and the results for the extended fire conditions (1500 F for 86 minutes) that exist in a long term storage facility and concludes that the 9975 packaging Pu/SS interface temperature is well below the eutectic temperature.

  10. Is PiSS Alpha-1 Antitrypsin Deficiency Associated with Disease?

    Directory of Open Access Journals (Sweden)

    Dawn McGee

    2010-01-01

    Full Text Available Background. Alpha-1 antitrypsin deficiency (AAT is an inherited condition that predisposes to lung and/or liver disease. Objective. The current study examined the clinical features of the PiSS genotype. Methods. Nineteen study participants (PiSS and 29 matched control participants (PiMM were telephone interviewed using a standardized questionnaire. Demographic features, cigarette smoking, vocation, medication history, and clinical diagnoses were compared. Statistical analysis was performed. Finally, a comprehensive literature review was performed by two investigators. Results. 12/19 (63.2% study participants reported the presence of lung and/or liver disease compared to 12/29 (41.4% control participants. There trended toward having a higher frequency of medication allergies in the study population (42.11% versus 20.69%. Conclusions. The PiSS genotype was associated with a similar incidence of obstructive lung disease to controls. Selective bias intrinsic in testing for AAT deficiency and the rarity of the PiSS genotype will make future study of this association dependent on population-based tests.

  11. Possibility of Inducing Compressive Residual Stresses in Welded Joints of SS400 Steels

    Institute of Scientific and Technical Information of China (English)

    2001-01-01

    Since the welded constructions produce easily stress corrosion cracking (SCC) or fatigue disruption in corrosive medium or under ripple load, two methods inducing compressive stress on structural surfaces by anti-welding-heating treatment (AWHT) and explosion treatment (ET) are presented. The results show that they are good ways to resisting SCC on the welded SS400 steel or other components.

  12. Agilent acceSS7网络监测的卫兵

    Institute of Scientific and Technical Information of China (English)

    2001-01-01

    @@ 网络监测系统的研发是安捷伦科技业务构成中的一个重要组成部分.近年来,以其骄人的成果在业界树立了良好的形象.位于北京的安捷伦科技软件设计中心成立于1997年,是安捷伦科技软件研究核心机构,中心研制的SS7监控系统acceSS7是专为电信运营商量身定做的应用方案,已经被业界广泛认可并归纳为基于SS7的电信网络监控和数据采集的标准.该方案在大小网络、无线和有线应用等领域占有主导地位,目前在全球范围内,安装Agilent acceSS7的客户已经超过70家.

  13. Summary of Tests to Determine Effectiveness of Gelatin Strike on SS{ampersand}C Dissolver Solutions

    Energy Technology Data Exchange (ETDEWEB)

    Murray, A.M. [Westinghouse Savannah River Company, AIKEN, SC (United States); Karraker, D.G.

    1998-05-01

    The solutions from the dissolution of sand, slag, and crucible (SS&C) material are sufficiently different from previous solutions processed via the F-Canyon Purex process that the effectiveness of individual process steps needed to be ascertained. In this study, the effectiveness of gelatin strike was tested under a variety of conditions. Specifically, several concentrations of silica, fluoride, nitric acid (HNO{sub 3}), boric acid (H{sub 3}BO{sub 3}), and aluminium nitrate nonahydrate (ANN) were studied. The disengagement times of surrogate and plant SS&C dissolver solutions from plant solvent also were measured. The results of the tests indicate that gelatin strike does not coagulate the silica at the low concentration of silica ({tilde 30} ppm) expected in the SS&C dissolver solutions because the silicon is complexed with fluoride ions (e.g., SiF{sub 6}{sup -2}). The silicon fluoride complex is expected to remain with the aqueous phase during solvent extraction. The disengagement times of the dissolver solutions from the plant solvent were not affected by the presence of low concentrations of silica and no third phase formation was observed in the disengagement phase with the low silica concentrations. Tests of surrogate SS&C dissolver solutions with higher concentration of silica (less than 150 ppm) did show that gelatin strike followed by centrifugation resulted in good phase disengagement of the surrogate SS{ampersand}C dissolver solution from the plant dissolver solution. At the higher silica concentrations, there is not sufficient fluoride to complex with the silica, and the silica must be entrained by the gelatin and removed from the dissolver solution prior to solvent extraction.

  14. Design and Hardware Implementation of QoSS - AES Processor for Multimedia applications

    Directory of Open Access Journals (Sweden)

    Zeghid Medien

    2010-04-01

    Full Text Available For real-time applications, there are several factors (time, cost, power that are moving security considerations from a function centric perspective into a system architecture (hardware/software design issue. Advanced Encryption Standard (AES is used nowadays extensively in many network and multimedia applications to address security issues. The AES algorithm specifies three key sizes: 128, 192 and 256 bits offering different levels of security. To deal with the amount of application and intensive computation given by security mechanisms, we define and develop a QoSS (Quality of Security Service model for reconfigurable AES processor. QoSS has been designed and implemented to achieve a flexible trade-off between overheads caused by security services and system performance. The proposed architecture can provide up to 12 AES block cipher schemes within a reasonable hardware cost. We envisage a security vector in a fully functional QoSS request to include levels of service for the range of security service and mechanisms. Our unified hardware can run both the original AES algorithm and the extended AES algorithm (QoSS-AES. A novel on-the-fly AES encryption/ decryption design is also proposed for 128- , 192- , and 256-bit keys. The performance of the proposed processor has been analyzed in an MPEG4 video compression standard. The results revealed that the QoSS-AES processor is well suited to provide high security communication with low latencies. In our implementation based on Xilinx Virtex FPGAs, speed/area/power results from these processors are analyzed and shown to compare favorably with other well known FPGA based implementations.

  15. First insights into the genetic diversity of Echinococcus granulosus sensu stricto (s.s.) in Serbia.

    Science.gov (United States)

    Debeljak, Zoran; Boufana, Belgees; Interisano, Maria; Vidanovic, Dejan; Kulisic, Zoran; Casulli, Adriano

    2016-06-15

    Cystic echinococcosis (CE) caused by Echinococcus granulosus sensu stricto (s.s.) is a cosmopolitan zoonotic infection which is endemic in Serbia where it is subject to mandatory reporting. However, information on the incidence of the disease in humans and prevalence of hydatid infection in livestock remains limited. We used sequenced data of the cytochrome c oxidase subunit 1 (cox 1) mitochondrial gene to examine the genetic diversity and population structure of E. granulosus (s.s.) from intermediate hosts from Serbia. We also compared our generated nucleotide sequences with those reported for neighbouring European countries. Echinococcus canadensis was molecularly confirmed from pig and human hydatid isolates. E. granulosus (G1) was confirmed from sheep and cattle hydatid isolates as well as the first molecular confirmation in Serbia of E. granulosus G2 in sheep and E. granulosus G3 in sheep and cattle hydatid isolates. The Serbian E. granulosus (s.s.) parsimony network displayed 2 main haplotypes (SB02 and SB05) which together with the neutrality indices were suggestive of bottleneck and/or balancing selection. Haplotype analysis showed the presence of the common E. granulosus haplotype described from other worldwide regions. Investigation of the pairwise fixation (Fst) index suggested that Serbian populations of E. granulosus (s.s.) from sheep and cattle hosts showed moderate genetic differentiation. Six of the Serbian haplotypes (SB02-SB07) were shared with haplotypes from Bulgaria, Hungary and/or Romania. Further studies using a larger number of hydatid isolates from various locations across Serbia will provide more information on the genetic structure of E. granulosus (s.s.) within this region.

  16. Fatigue Crack Propagation from Notched Specimens of 304 SS in elevated Temperature Aqueous Environment

    Energy Technology Data Exchange (ETDEWEB)

    Wire, G. L.; Mills, W. J.

    2002-08-01

    Fatigue crack propagation (FCP) rates for 304 stainless steel (304SS) were determined in 24 degree C and 288 degree C air and 288 degree C water using double-edged notch (DEN) specimens of 304 stainless steel (304 SS). Test performed at matched loading conditions in air and water at 288 degree C with 20-6- cc h[sub]2/kg h[sub]2O provided a direct comparison of the relative crack growth rates in air and water over a wide range of crack growth rates. The DEN crack extension ranged from short cracks (0.03-0.25 mm) to long cracks up to 4.06 mm, which are consistent with conventional deep crack tests. Crack growth rates of 304 SS in water were about 12 times the air rate. This 12X environmental enhancement persisted to crack extensions up to 4.06 mm, far outside the range associated with short crack effects. The large environmental degradation for 304 SS crack growth is consistent with the strong reduction of fatigue life in high hydrogen water. Further, very similar environmental effects w ere reported in fatigue crack growth tests in hydrogen water chemistry (HWC). Most literature data in high hydrogen water show only a mild environmental effect for 304 SS, of order 2.5 times air or less, but the tests were predominantly performed at high cyclic stress intensity or equivalently, high air rates. The environmental effect in low oxygen environments at low stress intensity depends strongly on both the stress ratio, R, and the load rise time, T[sub]r, as recently reported for austenitic stainless steel in BWR water. Fractography was performed for both tests in air and water. At 288 degree C in water, the fracture surfaces were crisply faceted with a crystallographic appearance, and showed striations under high magnification. The cleavage-like facets on the fracture surfaces suggest that hydrogen embrittlement is the primary cause of accelerated cracking.

  17. Improvisointi kanttorin työssä : "...lupa olla välittämässä sitä virtaa, joka on olemassa."

    OpenAIRE

    Perko, Johannes

    2011-01-01

    Tämän opinnäytetyön tavoitteena oli kartoittaa improvisoinnin käytettävyyttä ja käyttökokemuksia kanttorin työssä. Aluksi luodaan yleiskuva kanttorin viran historiaan ja improvisoinnin opetukseen keskeisissä kirkkomusiikkikouluissa. Improvisaation käsitettä avataan yksittäisten muusikoiden kokemuksilla. Muusikoita oli haastateltu RONDO-lehden artikkeleissa. Kanttorien improvisointikäytäntöjä on tutkittu vähän. Siksi tämä tutkielma sisältää kyselyn, joka lähetettiin Turun arkkihiippakunnan...

  18. Escherichia coli RecO protein anneals ssDNA complexed with its cognate ssDNA-binding protein: A common step in genetic recombination

    OpenAIRE

    Kantake, Noriko; Madiraju, Murty V. V. M.; Sugiyama, Tomohiko; Kowalczykowski, Stephen C.

    2002-01-01

    We present biochemical evidence for the functional similarity of Escherichia coli RecO protein and bacteriophage T4 UvsY protein to eukaryotic Rad52 protein. Although Rad52 protein is conserved in eukaryotes, no sequence homologue has been found in prokaryotes or archeabacteria. Rad52 protein has two unique activities: facilitation of replication protein-A (RPA) displacement by Rad51 protein and annealing of RPA–single-stranded DNA (ssDNA) complexes. Both activities require species-specific i...

  19. On Strength Analyzing of Strctures for SS7 Canon Box%SS7抱轴箱体的结构强度分析

    Institute of Scientific and Technical Information of China (English)

    郭同生; 杨俊杰

    2009-01-01

    利用有限元法对韶山7型电力机车抱轴箱体3种结构的静强度、模态和动态响应进行分析.结果表明,局部几何性应力集中对过早裂损有一定影响,但影响不大;引起SS7抱轴箱体裂纹的原因是结构设计以外的其他原因.

  20. "Lähtökohtana kohdata ihminen ihmisenä" - : käsityksiä monikulttuurisesta osaamisesta etsivässä nuorisotyössä Helsingissä

    OpenAIRE

    Pulli, Jatta

    2013-01-01

    ssä opinnäytetyössä tarkastellaan käsityksiä monikulttuurisesta osaamisesta etsivässä nuorisotyössä Helsingissä. Monikulttuurisesta nuorisotyöstä on olemassa useita tutkimuksia ja samaten etsivästä nuorisotyöstä on kirjoitettu ja siitä ollaan tietoisia, varsinkin etsivän nuorisotyön Nuorisolakiin lisäyksen myötä 2011. Näitä kahta yhdessä ei kuitenkaan ole tarkasteltu paljoakaan. Tavoitteena opinnäytetyössä on saada tietää käytännön etsivää nuorisotyötä tekeviltä tahoilta, miten he miel...

  1. [Psychogram of the Auschwitz commander: insight and confrontation through destruction. On the autobiography of Rudolf Höss].

    Science.gov (United States)

    Zeiler, J

    1991-04-01

    By reference to the personality of Rudolf Höss, the author illuminates once again the terrifying juxtaposition of criminality and apparent normality in the psychic life of a Nazi criminal. The inferno of the concentration camp is interpreted as a perverse explication and staging of split-off ideational contents in the mind of Höss.

  2. D. Larsen: Fortrængt Grusomhed (Dänische SS-Wächter 1941-45)

    DEFF Research Database (Denmark)

    Hansen, Jens-Christian

    2011-01-01

    Anmeldelse af Dennis Larsens bog "Fortrængt grusomhed. Danske SS-vagter 1941-45" for H-Soz-u-Kult......Anmeldelse af Dennis Larsens bog "Fortrængt grusomhed. Danske SS-vagter 1941-45" for H-Soz-u-Kult...

  3. ss-siRNAs allele selectively inhibit ataxin-3 expression: multiple mechanisms for an alternative gene silencing strategy.

    Science.gov (United States)

    Liu, Jing; Yu, Dongbo; Aiba, Yuichiro; Pendergraff, Hannah; Swayze, Eric E; Lima, Walt F; Hu, Jiaxin; Prakash, Thazha P; Corey, David R

    2013-11-01

    Single-stranded silencing RNAs (ss-siRNAs) provide an alternative approach to gene silencing. ss-siRNAs combine the simplicity and favorable biodistribution of antisense oligonucleotides with robust silencing through RNA interference (RNAi). Previous studies reported potent and allele-selective inhibition of human huntingtin expression by ss-siRNAs that target the expanded CAG repeats within the mutant allele. Mutant ataxin-3, the genetic cause of Machado-Joseph Disease, also contains an expanded CAG repeat. We demonstrate here that ss-siRNAs are allele-selective inhibitors of ataxin-3 expression and then redesign ss-siRNAs to optimize their selectivity. We find that both RNAi-related and non-RNAi-related mechanisms affect gene expression by either blocking translation or affecting alternative splicing. These results have four broad implications: (i) ss-siRNAs will not always behave similarly to analogous RNA duplexes; (ii) the sequences surrounding CAG repeats affect allele-selectivity of anti-CAG oligonucleotides; (iii) ss-siRNAs can function through multiple mechanisms and; and (iv) it is possible to use chemical modification to optimize ss-siRNA properties and improve their potential for drug discovery.

  4. Cyclic lipopeptide surfactant production by Pseudomonas fluorescens SS101 is not required for suppression of complex Pythium spp. populations

    Science.gov (United States)

    Previously, zoosporicidal activity and control of Pythium intermedium by Pseudomonas fluorescens strain SS101 was attributed, in part, to the production of the cyclic lipopeptide surfactant massetolide A. In the current study, capacity of SS101 and its surfactant-deficient mutant strain 10.24 to sup...

  5. Molecular cloning, characterization and differential expression of a Sporothrix schenckii STE20-like protein kinase SsSte20.

    Science.gov (United States)

    Zhang, Zhenying; Hou, Binbin; Zheng, Fangliang; Yu, Xiaohong; Liu, Xiaoming

    2013-06-01

    Dimorphic switching requires fungal cells to undergo changes in polarized growth in response to environmental stimuli. The Ste20-related kinases are involved in signaling through mitogen-activated protein kinase pathways and in morphogenesis through the regulation of cytokinesis and actin-dependent polarized growth. In this report, we isolated and characterized an Ste20 homologue gene, designated SsSte20, from yeast-form Sporothrix schenckii (S. schenckii). The full length SsSte20 cDNA sequence is 2846 bp in size, and contains an open reading frame of 2505 bp encoding 835 amino acids. The predicted molecular mass of SsSte20 is 91.31 kDa with an estimated theoretical isoelectric point of 5.76. SsSte20 kinase domain shows 63% identity with that of Don3, a germinal centre kinase (GCK) from Ustilago maydis. Two exons and one intron are identified within the 2578 bp SsSte20 genomic DNA sequence of S. schenckii. Differential expression of the SsSte20 was demonstrated by real-time RT-PCR. The expression of SsSte20 was much higher in the yeast stage compared with that in the mycelial stage, which indicated that the SsSte20 may be involved in the pathogenesis of the yeast phase of S. schenckii.

  6. Self-assembly of ssDNA-amphiphiles into micelles, nanotapes and nanotubes

    Science.gov (United States)

    Pearce, Timothy R.

    The field of DNA nanotechnology utilizes DNA as a construction material to create functional supramolecular and multi-dimensional structures like two-dimensional periodic lattices and three-dimensional polyhedrons with order on the nanometer scale for many nanotechnology applications including molecular templating, nanosensors, and drug delivery. Single-stranded DNA (ssDNA) is often used to create these nanostructures as the DNA bases provide an intrinsic molecular code that can be exploited to allow for the programmed assembly of structures based upon Watson-Crick base-pairing. However, engineering these complex structures from biopolymers alone requires careful design to ensure that the intrinsic forces responsible for organizing the materials can produce the desired structures. Additional control over supramolecular assembly can be achieved by chemically modifying the ssDNA with hydrophobic moieties to create amphiphilic molecules, which adds the hydrophobic interaction to the list of contributing forces that drive the self-assembly process. We first explored the self-assembly behavior of a set of ssDNA aptamer-amphiphiles composed of the same hydrophobic tail and hydrophilic ssDNA aptamer headgroup but with different spacer molecules linking these groups together. Through the use of cryo-transmission electron microscopy (cryo-TEM), small angle x-ray scattering (SAXS), and circular dichroism (CD) we show that the aptamer-amphiphiles can assemble into a variety of structures depending on the spacer used. We demonstrated, for the first time, the creation of self-assembled aptamer-amphiphile nanotape structures and show that the choice of the spacer used in the design of aptamer-amphiphiles can influence their supramolecular self-assembly as well as the secondary structure of the aptamer headgroup. We next explored the role of the ssDNA headgroup on the amphiphile self-assembly behavior by designing amphiphiles with headgroups of multiple lengths and nucleotides

  7. Expanding the scope of CE reactor to ssDNA-binding protein-ssDNA complexes as exemplified for a tool for direct measurement of dissociation kinetics of biomolecular complexes.

    Science.gov (United States)

    Takahashi, Toru; Ohtsuka, Kei-Ichirou; Tomiya, Yoriyuki; Iki, Nobuhiko; Hoshino, Hitoshi

    2009-09-01

    CE reactor (CER), which was developed as a tool for direct measurement of the dissociation kinetics of metal complexes, was successfully applied to the complexes of Escherichia coli ssDNA-binding protein (SSB) with ssDNA. The basic concept of CER is the application of CE separation process as a dissociation kinetic reactor for the complex, and the observation of the on-capillary dissociation reaction profile of the complex as the decrease of the peak height of the complex with increase of the migration time. The peak height of [SSB-ssDNA] decreases as the migration time increases since the degree of the decrease of [SSB-ssDNA] through the on-capillary dissociation reaction is proportional to the degree of the decrease of the peak height of [SSB-ssDNA]. The dissociation degree-time profiles for the complexes are quantitatively described by analyzing a set of electropherograms with different migration times. Dissociation rate constants of [SSB-ssDNA] consisting of 20-mer, 25-mer and 31-mer ssDNA were directly determined to be 3.99x10(-4), 4.82x10(-4) and 1.50x10(-3)/s, respectively. CER is a concise and effective tool for dissociation kinetic analysis of biomolecular complexes.

  8. Expression and Its Regulation of KiSS-1 Gene Related with Reproduction%KiSS-1基因与生殖相关的表达及其调节

    Institute of Scientific and Technical Information of China (English)

    王军; 孙蕾; 常喆

    2011-01-01

    Recently, it has been found that KiSS-1 gene plays important roles in mammalian reproduction. Kisspeptin, the peptide product of the KiSS-1 gene has been shown to be powerful stimulators of the gonadotropin. Recent advances of expression of KiSS-1 gene related with reproduction and its regulation of hypothalamic expression by sex steroid hormones, metabolic hormone and photoperiod were reviewed in this paper for providing a reference for the direction of further study of KiSS-1 gene.%最近研究发现,KiSS-1基因在哺乳动物生殖内分泌的调节中具有重要作用,其编码的产物——kisspeptin能够强有力地刺激促性腺激素的分泌.笔者综述了KiSS-1基因与生殖相关的表达及性腺类固醇激素、代谢激素与光周期对其在下丘脑表达调节的最新研究进展,为今后KiSS-1基因的研究取向提供参考.

  9. 姬松茸多糖对铅中毒大鼠铅代谢及体内铜、钙、锌、铁、锰含量的影响%EFFECTS OF AGARICUS BLAZEI MUILL POLYSACCHARIDES ON LEAD METABOLISM AND THE CONTENTS OF COPPER, CALCIUM, ZINC, IRON AND MANGANESE IN LEAD-POISONING RATS

    Institute of Scientific and Technical Information of China (English)

    程红艳; 冯翠萍; 王伟娟; 常明昌; 孟俊龙; 冯两蕊

    2012-01-01

    目的 了解姬松茸多糖(Agaricus blazei Murrill polysaccharides,ABP)对Pb中毒大鼠体内Pb、Cu、Ca、Zn、Fe、Mn含量的影响.方法 选择健康45日龄SD大鼠48只,随机分为6组,每组8只,雌雄各半,分别为正常对照组、Pb中毒组、多糖组、Pb中毒+低剂量多糖干预组、Pb中毒+中剂量多糖干预组和Pb中毒+高剂量多糖干预组.Pb中毒组和Pb中毒+多糖干预组都给予0.2%醋酸铅溶液,自由饮用,其它组给予蒸馏水.多糖、低剂量、中剂量及高剂量组分别按每天每只100、50、100、200mg/kg体重灌服姬松茸多糖液,各组灌服液体积相等,为1ml.正常对照组和Pb中毒组每天每只灌胃2ml的生理盐水.饲养60d后,取大鼠肝脏、肾脏、心脏和血,分别测定Pb、Cu、Ca、Zn、Fe、Mn含量.结果 (1)与正常对照组比较,Pb中毒组各组织和三个多糖干预组中肝脏、肾脏和血中Pb含量升高,差异具有统计学意义(P<0.01),Pb中毒组心脏中的Ca、血中的Fe含量升高(P<0.05或P<0.01),其余均下降(P<0.05或P<0.01),多糖组中肾脏和心脏中的Ca,血中的Fe略有下降,其余均略有升高,差异无统计学意义.(2)与Pb中毒组比较,低剂量多糖显著降低血铅含量(P<0.01),中剂量多糖显著降低心脏中铅含量(P<0.01),其他组织铅含量差异无统计学意义,随着ABP剂量的升高,三个多糖干预组中心脏中的Ca和血中的Fe的含量逐渐降低,而其他指标均逐渐升高,当ABP剂量达到200mg/kg时,显著下降或升高(P<0.05或P<0.01).结论 姬松茸多糖促进Pb的排出,对Pb致大鼠微量元素失衡具有调节作用.%Objective To investigate the effects of Agaricus blazei Murrill polysaccharides (ABP) on Pb metabolism and the contents of Pb, Cu, Ca, Zn, Fe and Mn in lead-poisoning rats. Method Forty eight healthy 45 d SD rats were randomly divided into 6 groups (female ; male=1:1): control group, ABP group, lead-poisoning group, lead+low-dose ABP group

  10. Quantifying the Carbon Abundances in the Secondary Stars of SS Cygni, RU Pegasi, and GK Persei

    CERN Document Server

    Harrison, Thomas E

    2015-01-01

    We use a modified version of MOOG to generate large grids of synthetic spectra in an attempt to derive quantitative abundances for three CVs (GK Per, RU Peg, and SS Cyg) by comparing the models to moderate resolution (R $\\sim$ 25,000) $K$-band spectra obtained with NIRSPEC on Keck. For each of the three systems we find solar, or slightly sub-solar values for [Fe/H], but significant deficits of carbon: for SS Cyg we find [C/Fe] = $-$0.50, for RU Peg [C/Fe] = $-$0.75, and for GK Per [C/Fe] = $-$1.00. We show that it is possible to use lower resolution (R $\\sim$ 2,000) spectra to quantify carbon deficits. We examine realistic veiling scenarios and find that emission from H I or CO cannot reproduce the observations.

  11. The algebra of observables in Gau{\\ss}ian normal spacetime coordinates

    CERN Document Server

    Bodendorfer, Norbert; Lewandowski, Jerzy; Świeżewski, Jędrzej

    2016-01-01

    We discuss the canonical structure of a spacetime version of the radial gauge, i.e. Gau{\\ss}ian normal spacetime coordinates. While it was found for the spatial version of the radial gauge that a "local" algebra of observables can be constructed, it turns out that this is not possible for the spacetime version. The technical reason for this observation is that the new gauge condition needed to upgrade the spatial to a spacetime radial gauge does not Poisson-commute with the previous gauge conditions. It follows that the involved Dirac bracket is inherently non-local in the sense that no complete set of observables can be found which is constructed locally and at the same time has local Dirac brackets. A locally constructed observable here is defined as a finite polynomial of the canonical variables at a given physical point specified by the Gau{\\ss}ian normal spacetime coordinates.

  12. Salassapidon haasteet työelämässä

    OpenAIRE

    Puolatie, Miia

    2013-01-01

    Sosiaalisen median vaikutus kasvaa vuosi vuodelta enemmän. Salassapidon haasteet kasvavat näiden sosiaalisten medioiden vaikutusten myötä. Työsopimuslain mukaan työnantajan liike – ja ammattisalaisuuksia ei saa kertoa eteenpäin, eikä saa yrittää tahallisesti hyötyä näistä tiedoista. Laki ei kuitenkaan kerro mitä tällaiset liike – ja ammattisalaisuudet ovat. Tässä työssä tutustuttiin salassapidon haasteisiin verraten työsopimuslakia työntekijöiden käytännön tietoihin. Samalla myös tutkittii...

  13. Uncertainty Estimation of Analysis of Hg in Fish and Shrimp Sample by SS-AAS Method

    International Nuclear Information System (INIS)

    An uncertainty estimation of analysis of Hg in Fish and Shrimp sample by SS-AAS method has been done. Samples preparation have been done by using freeze drying. They were then homogenized and sieved, so their grain size of 100 mesh were obtained. The content of Hg in the samples and SRM 1566a has been analyzed by using SS-AAS (solid sampling - atomic absorption spectrometer) AAS 5 EA, ZEISS. The experimental result shows that expanded uncertainty value of fish sample is 4.3636, whereas expanded uncertainty value of shrimp’s sample = 2.638. Precision value is = 98.46 % and accuracy value = 97.0 % and limit of detection = 0.0154 ng/g. (author)

  14. Wear and Corrosion Study of Sputtered Zirconium thin films on SS316L for Windmill Application

    Directory of Open Access Journals (Sweden)

    Arunkumar N

    2015-05-01

    Full Text Available The Aim of this study is to observe the Wear and Corrosion behavior of Zirconium coated 316L stainless steel. After polishing, SS316L was coated with Zirconium employing DC sputtering process (a technique of physical vapor deposition.Structure characterization techniques including Scanning Electron Microscope (SEM and X-Ray Diffraction (XRD were utilized to investigate the microstructure and crystallinity of the coating. Salt spray test was performed by spraying Sodium chloride in order to determine corrosion resistance behavior of the coated sample. Pin on disc wear test was performed by hardened and tempered EN31 steel pin in order to determine and compare the Wear resistance behavior of Coated and uncoated samples. The Objective is to recommend the zirconium coated Stainless steel SS316L can be a choice for Off-shore wind mills where the shafts undergo Wear and corrosion problems.

  15. Valtion metsien kehitys Kittilässä vuodesta 1924 vuoteen 2011

    OpenAIRE

    Vaara, Ilkka

    2012-01-01

    Metsähallituksen ensimmäisiä tehtäviä perustamisen jälkeen oli hankkia tiedot maa- ja metsäomaisuudesta. Vuosisadan vaihteen jälkeen arviointeja alettiin tehdä 10 – 20 vuoden välein. Paikkatietotietojärjestelmien kehittymi-sen myötä jatkuvaan ylläpitoon siirryttiin 1990-luvulla Tässä opinnäytetyössä pääasiallisena tietolähteenä ovat Metsähallituksen Kittilän hoitoalueen metsätaloussuunnitelmat sekä nykyiset maankäyttö- ja kuviotiedot. Koko Suomea koskeva tarkastelu perustuu pääasiassa Me...

  16. Pairing Mismatched ssDNA to dsDNA Studied with Reflectometric Interference Spectroscopy Sensor

    Institute of Scientific and Technical Information of China (English)

    Qing-Qing Wu; Kai-Ge Wang; Dan Sun; Shuang Wang; Chen Zhang; Wei Zhao

    2016-01-01

    The interaction between two single-stranded DNA (ssDNA) molecules as pairing to a double-stranded DNA (dsDNA) molecule is studied by the reflectometric interference spectroscopy (RIFS) technology.A nano-porous anode alumina membrane coated an Au layer is employed as the sensor substrate.The results indicate that when there are mismatched nucleotide bases,the effective optical thicknesses (OTeff) have obvious difference,and the changes of OTeff are connected with the sensor layer thickness and the effective refractive index.It is also demonstrated that the RIFS technique can be used to precisely detect the ssDNA molecules with individual base mismatched as pairing to dsDNA.

  17. Teatterisavumerkkejä - Kommunikointi työryhmässä

    OpenAIRE

    Nenonen, Camilla

    2011-01-01

    BA-opinnäytetyössä Teatterisavumerkkejä – Kommunikointi työryhmässä tarkasteltiin suunnittelijatyöryhmän kommunikointia teatterilavastajan näkökulmasta. Havainnot olivat pääosin tekijän taiteellisen opinnäytteen YOU’RE MY HIT AND YOU’RE MY SHIT tekoprosessista. Lisäksi viitattiin muihin produktioihin, joissa tekijä oli ollut mukana, sekä teatterin ammattilaisten näkemyksiin aiheesta. Opinnäytteen tavoitteena oli oppia enemmän lavastajan roolista taiteellisen työryhmän jäsenenä ja sen myötä ke...

  18. CLaSS Computer Literacy Software: From Design to Implementation - A Three Year Student Evaluation

    Directory of Open Access Journals (Sweden)

    Ian Cole

    2006-12-01

    Full Text Available Both computer literacy and information retrieval techniques are required to undertake studies in higher education in the United Kingdom. This paper considers the research, development and the 3-year student evaluation of a piece of learning technology in computer and information literacy (CLaSS software. Students completed a questionnaire to examine their own assessment of knowledge and competence in computer and information literacy and based on this assessment CLaSS software was created to assist nursing students with computer and information literacy. This paper draws on existing literature and applies a specific learning model to the software while considering software engineering and user-centered design methodologies. The technical processes involved in designing and creating the software are briefly considered with software development data analysis discussed. A 3-year student evaluation of the software after it's release was undertaken to consider the long-term validity and usefulness of this software with the results analysed and discussed.

  19. The SSES-E: a measure of sexual self-efficacy in erectile functioning.

    Science.gov (United States)

    Libman, E; Rothenberg, I; Fichten, C S; Amsel, R

    1985-01-01

    The purpose of this study was to develop and validate the Sexual Self-Efficacy Scale (SSES-E) for erectile disorder. The subjects consisted of 15 heterosexual couples with nonproblematic sexual functioning (normal group) and a sexually dysfunctional sample consisting of nine heterosexual couples and eight heterosexual single males (dysfunctional group). Most of the males in the dysfunctional sample were diagnosed as suffering from erectile disorder. Reliability of the SSES-E, based on test-retest and split-half correlations and on item analyses, appears to be reasonable. Validity, measured in three different ways, is also acceptably high. Suggestions for the use of this instrument in clinical practice and for future research are made.

  20. Crack propagation tests of HIPed DSCu/SS joints for plasma facing components

    International Nuclear Information System (INIS)

    Aluminum oxide dispersion strengthened copper (DSCu), used as the heat sink, and AISI 316L(N) type stainless steel (SS), used for cooling pipes, were metallurgically joined to be used as first wall of plasma facing components. Hot isostatic pressing (HIP) was proposed as the joining fabrication technique in the first-wall/blanket components. In this study, fracture toughness and fatigue crack growth rate tests were carried out for the fracture strength evaluation of HIPed joints. Permissible crack lengths during fabrication of first-wall components were evaluated from the results of the fracture toughness tests. In crack growth rate tests, the crack prepared in the DSCu propagated, turned at the HIPed interface, and propagated along it. Therefore, it was found that the crack provided in the DSCu heat sink did not propagate through the SS cooling pipe

  1. An accurate geometric distance to the compact binary SS Cygni vindicates accretion disc theory

    CERN Document Server

    Miller-Jones, J C A; Knigge, C; Körding, E G; Templeton, M; Waagen, E O

    2013-01-01

    Dwarf novae are white dwarfs accreting matter from a nearby red dwarf companion. Their regular outbursts are explained by a thermal-viscous instability in the accretion disc, described by the disc instability model that has since been successfully extended to other accreting systems. However, the prototypical dwarf nova, SS Cygni, presents a major challenge to our understanding of accretion disc theory. At the distance of 159 +/- 12 pc measured by the Hubble Space Telescope, it is too luminous to be undergoing the observed regular outbursts. Using very long baseline interferometric radio observations, we report an accurate, model-independent distance to SS Cygni that places the source significantly closer at 114 +/- 2 pc. This reconciles the source behavior with our understanding of accretion disc theory in accreting compact objects.

  2. Siivoustyön kehittäminen Campusravita Oy:ssä : Esivalmisteltu siivous

    OpenAIRE

    Matilainen, Mari

    2014-01-01

    Työn tavoitteena oli tehdä Campusravita Oy:lle uusi siivoussuunnitelma. Tavoitteena oli tehdä suunnitelma joka mukailee esivalmistellun siivouksen periaatteita. Tässä työssä huomioitiin kestävä kehitys jätteen ja veden osalta. Näiden lisäksi haluttiin osoittaa syntyneet säästöt ajallisesti ja taloudellisesti. Laskelmilla pyrittiin näyttämään kuinka paljon kuluu rahaa eri siivousmenetelmillä ja vertailla niitä keskenään. Työn tavoitteena oli löytää mahdollisimman toimiva ratkaisu Campusr...

  3. Hydrogen–deuterium exchange on plasma-exposed W and SS surface

    Energy Technology Data Exchange (ETDEWEB)

    Takagi, Ikuji, E-mail: takagi@nucleng.kyoto-u.ac.jp; Nomura, Shinji; Minamimoto, Toshihiro; Akiyoshi, Masafumi; Kobayashi, Taishi; Sasaki, Takayuki

    2015-08-15

    The desorption cross section for hydrogen isotopes adsorbed on stainless steel (SS) and tungsten (W) has been evaluated experimentally to provide basic information on tritium exchange. One side of a sample sheet was alternately exposed to H and D plasma, and deuterium density on the surface was repeatedly observed using nuclear reaction analysis (NRA) under continuous plasma exposure. From the time dependent change in the deuterium density, the desorption cross sections for SS and W were estimated to be 6.9 ± 2.3 × 10{sup −23} m{sup 2} and 4.6 ± 1.0 × 10{sup −23} m{sup 2}, respectively. No significant differences in the cross section between H and D plasma were observed. Recombinative desorption was found to dominate the desorption process owing to the low incident energy of hydrogen atoms.

  4. Työaika-autonomia ja Mansikkapaikan palvelukotien hoitajien työssä jaksaminen

    OpenAIRE

    Kokkomäki, Saana

    2015-01-01

    Opinnäytetyön tarkoituksena oli kehittää edelleen Rauman Mansikkapaikan palvelukotien hoitajien käytössä olevaa työaika-autonomista työvuorosuunnittelua. Tavoitteena oli saada tietoa työaika-autonomisen työvuorosuunnittelun hyvistä käytännöistä sekä tunnistaa mahdolliset kehittämistarpeet. Tavoitteena oli myös kuvata Mansikkapaikan palvelukotien hoitajien kokemuksia työaika-autonomian merkityksestä työssä jaksamiseen. Opinnäytetyön tutkimusmenetelmänä käytettiin Delfoi-menetelmää. Ainei...

  5. EVALUATION OF MECHANICAL PROPERTIES OF SS 316 L WELDMENTS USING TUNGSTEN INERT GAS WELDING

    Directory of Open Access Journals (Sweden)

    S.V. ABINESH KUMAR

    2012-05-01

    Full Text Available This work aims at joining of similar grades of stainless steel by TIG welding with the various parameters like current, bevel angle and gas flow rate. The SS 316L is selected over other grades due to its lesser carbon content it is used in pressure vessels for corrosive liquids etc. The rod of SS 316L of 25 mm diameter and 75 mm lengthwas used as the base material for this experiment. The rod was machined in accordance to ASTM-A-370 standards for finding the echanical behaviour like Tensile strength, micro hardness and micro structure. Higher tensile strength achieved with a current of 110A, bevel angle of 600 and a gas flow rate of 0.7 LPM. Non-destructive tests like radiographic tests were performed to find the defects in the joints. The defect incurred was lack of penetration and it was observed in the sample D and sample G.

  6. SMS Hubbing的SS7漫游体系架构解决方案

    Institute of Scientific and Technical Information of China (English)

    冯筱佳

    2012-01-01

    本文将详细研究在SS7域中,SMSHubbing的漫游体系架构设计和解决方案。并对Hubbing方式和Bilateral/Multilateral方式从网络拓扑上进行了探讨。尤其是级联hub组网的情况下,需要对转发消息进行路由处理以实现透明性和开放性。接着对GSMA的国际漫游专家组IREG推荐的漫游场景进行详细分析,然后给出了SS7域中短信网关所要做的消息处理。

  7. Bioconversion of glycerol for bioethanol production using isolated Escherichia coli SS1

    Directory of Open Access Journals (Sweden)

    Sheril Norliana Suhaimi

    2012-06-01

    Full Text Available Bioconverting glycerol into various valuable products is one of glycerol's promising applications due to its high availability at low cost and the existence of many glycerol-utilizing microorganisms. Bioethanol and biohydrogen, which are types of renewable fuels, are two examples of bioconverted products. The objectives of this study were to evaluate ethanol production from different media by local microorganism isolates and compare the ethanol fermentation profile of the selected strains to use of glucose or glycerol as sole carbon sources. The ethanol fermentations by six isolates were evaluated after a preliminary screening process. Strain named SS1 produced the highest ethanol yield of 1.0 mol: 1.0 mol glycerol and was identified as Escherichia coli SS1 Also, this isolated strain showed a higher affinity to glycerol than glucose for bioethanol production.

  8. Analysis of metabolites in plasma reveals distinct metabolic features between Dahl salt-sensitive rats and consomic SS.13(BN) rats.

    Science.gov (United States)

    Wang, Le; Hou, Entai; Wang, Zhengjun; Sun, Na; He, Liqing; Chen, Lan; Liang, Mingyu; Tian, Zhongmin

    2014-07-18

    Salt-sensitive hypertension is a major risk factor for cardiovascular disorders. Our previous proteomic study revealed substantial differences in several proteins between Dahl salt-sensitive (SS) rats and salt-insensitive consomic SS.13(BN) rats. Subsequent experiments indicated a role of fumarase insufficiency in the development of hypertension in SS rats. In the present study, a global metabolic profiling study was performed using gas chromatography/mass spectrometry (GC/MS) in plasma of SS rats (n=9) and SS.13(BN) rats (n=8) on 0.4% NaCl diet, designed to gain further insights into the relationship between alterations in cellular intermediary metabolism and predisposition to hypertension. Principal component analysis of the data sets revealed a clear clustering and separation of metabolic profiles between SS rats and SS.13(BN) rats. 23 differential metabolites were identified (PSS rats. Pyruvate, which connects TCA cycle and glycolysis, was also increased in SS rats. Moreover, lower activity levels of fumarase, aconitase, α-ketoglutarate dehydrogenase and succinyl-CoA synthetase were detected in the heart, liver or skeletal muscles of SS rats. The distinct metabolic features in SS and SS.13(BN) rats indicate abnormalities of TCA cycle in SS rats, which may play a role in predisposing SS rats to developing salt-sensitive hypertension.

  9. Programmability and portability for exascale: Top down programming methodology and tools with StarSs

    OpenAIRE

    Subotic, Vladimir; Brinkmann, Steffen; Marjanovic, Vladimir; Badia, Rosa M.; Gracia, Jose; Niethammer, Christoph; Ayguade, Eduard; LABARTA, Jesus; Valero, Mateo

    2013-01-01

    StarSs is a task-based programming model that allows to parallelize sequential applications by means of annotating the code with compiler directives. The model further supports transparent execution of designated tasks on heterogeneous platforms, including clusters of GPUs. This paper focuses on the methodology and tools that complements the programming model forming a consistent development environment with the objective of simplifying the live of application developers.The programming envir...

  10. Das Kindersprachscreening (KiSS) - ein Verfahren zur Sprachstandserfassung vierjähriger Kinder

    OpenAIRE

    NEUMANN, K; Holler-Zittlau, I; Sick, U; Zaretsky, Y; Euler, HA

    2009-01-01

    Einleitung: Zur Feststellung von Sprachförder- oder -therapiebedarf eignet sich ein Sprachscreening, sofern es eine ausreichende Validität aufweist und über streng algorithmische Kriterien zur Auffälligkeitsklassifizierung verfügt. Material und Methode: Für eine hessenweite Erfassung der vorschulischen Sprachkompetenz wurde das psychometrisch geprüfte Kindersprachscreening (KiSS) entwickelt. 257 Kinder im Alter von 4;0 bis 4;5 Jahren wurden mit einer Testbatterie auf ihren Sprachstand ...

  11. Liiketoiminnan jatkuvuus IT – ympäristössä : Case: BASF

    OpenAIRE

    Orpana, Mari

    2010-01-01

    Yritykset ovat varautuneet puutteellisesti tietotekniikkaan liittyviin onnettomuuksiin, vaikka yritysten riippuvuus tietoteknisien järjestelmien käytettävyydestä on jatkuvasti kasvanut. Vakavien häiriötilanteiden sattuessa yritys on suurissa vaikeuksissa, jos liiketoiminnan jatkuvuussuunnitelmaa ei ole tehty. Vuonna 2008 BASF konsernin Pohjoismaiden suurimaassa tuotantolaitoksessa sattui kriittisessä prosessissa useamman päivän käyttökatkos IT – ympäristössä tapahtuneen virheen vuoksi. Tä...

  12. Naisten karkkikaupassa : kokemuksia myynnin ammattitutkinnosta kosmetiikan face to face -myyntityössä

    OpenAIRE

    Alasiurua, Riikka

    2012-01-01

    Kosmetiikan jälleenmyynti on uudessa tilanteessa kosmetiikkatuotteiden saatavuuden laajennuttua Internetin maailmaan. Myös kosmetiikkaan liittyvä informaatio on helposti kuluttajien ulottuvilla. Näin ollen kuluttajat eivät ole enää riippuvaisia kosmetiikkamyyjien osaamisesta. Koska verkkokauppojen hintataso on usein perinteisiä jälleenmyyntipisteitä alhaisempi, ainoa kilpailuetu kasvokkain tapahtuvassa myyntityössä on vuorovaikutus. Sen tärkeys korostuu erityisesti kosmetiikan myynnissä...

  13. Upper Mantle Discontinuity Structure Beneath the Western Atlantic Ocean and Eastern North America from SS Precursors

    Science.gov (United States)

    Schmerr, N. C.; Beghein, C.; Kostic, D.; Baldridge, A. M.; West, J. D.; Nittler, L. R.; Bull, A. L.; Montesi, L.; Byrne, P. K.; Hummer, D. R.; Plescia, J. B.; Elkins-Tanton, L. T.; Lekic, V.; Schmidt, B. E.; Elkins, L. J.; Cooper, C. M.; ten Kate, I. L.; Van Hinsbergen, D. J. J.; Parai, R.; Glass, J. B.; Ni, J.; Fuji, N.; McCubbin, F. M.; Michalski, J. R.; Zhao, C.; Arevalo, R. D., Jr.; Koelemeijer, P.; Courtier, A. M.; Dalton, H.; Waszek, L.; Bahamonde, J.; Schmerr, B.; Gilpin, N.; Rosenshein, E.; Mach, K.; Ostrach, L. R.; Caracas, R.; Craddock, R. A.; Moore-Driskell, M. M.; Du Frane, W. L.; Kellogg, L. H.

    2015-12-01

    Seismic discontinuities within the mantle arise from a wide range of mechanisms, including changes in mineralogy, major element composition, melt content, volatile abundance, anisotropy, or a combination of the above. In particular, the depth and sharpness of upper mantle discontinuities at 410 and 660 km depth are attributed to solid-state phase changes sensitive to both mantle temperature and composition, where regions of thermal heterogeneity produce topography and chemical heterogeneity changes the impedance contrast across the discontinuity. Seismic mapping of this topography and sharpness thus provides constraint on the thermal and compositional state of the mantle. The EarthScope USArray is providing unprecedented access to a wide variety of new regions previously undersampled by the SS precursors. This includes the boundary between the oceanic plate in the western Atlantic Ocean and continental margin of eastern North America. Here we use a seismic array approach to image the depth, sharpness, and topography of the upper mantle discontinuities, as well as other possible upper mantle reflectors beneath this region. This array approach utilizes seismic waves that reflect off the underside of a mantle discontinuity and arrive several hundred seconds prior to the SS seismic phase as precursory energy. In this study, we collected high-quality broadband data SS precursors data from shallow focus (4th root vespagrams to enhance the SS precursors and determine how they sample the mantle. Our data show detection of localized structure on the discontinuity boundaries as well as additional horizons, such as the X-discontinuity and a potential reflection from a discontinuity near the depth of the lithosphere-asthenosphere boundary. These structures are related to the transition from predominantly old ocean lithosphere to underlying continental lithosphere, as while deeper reflectors are associated with the subduction of the ancient Farallon slab. A comparison of the

  14. Echelle-MEPSICRON time-resolved spectroscopy of the dwarf nova SS Cygni

    International Nuclear Information System (INIS)

    High-dispersion time-resolved spectroscopy of the dwarf nova SS Cygni was obtained during two consecutive days at the end of an outburst. The emission lines present a double-peaked profile with a peak-to-peak separation of 526±14 km s-1. The lines have a complex structure which includes a narrow asymmetric component coming from a hot spot in the accretion disc or from the chromosphere of the red dwarf. (author)

  15. Echelle-MEPSICRON time-resolved spectroscopy of the dwarf nova SS Cygni

    Energy Technology Data Exchange (ETDEWEB)

    Echevarria, J.; Tapia, M. (Universidad Nacional Autonoma de Mexico, Baja California (Mexico). Inst. de Astronomia); Diego, F. (Institute of Oceanographic Sciences, Godalming (UK)); Costero, R.; Ruiz, E.; Salas, L.; Gutierrez, L.; Enriquez, R. (Universidad Nacional Autonoma de Mexico, Mexico City (Mexico). Inst. de Astronomia)

    1989-10-15

    High-dispersion time-resolved spectroscopy of the dwarf nova SS Cygni was obtained during two consecutive days at the end of an outburst. The emission lines present a double-peaked profile with a peak-to-peak separation of 526{plus minus}14 km s{sup -1}. The lines have a complex structure which includes a narrow asymmetric component coming from a hot spot in the accretion disc or from the chromosphere of the red dwarf. (author).

  16. Lapsen osallistumisen tarkastelu COSA 2.2 -menetelmässä

    OpenAIRE

    Korpelainen, Petra; Levomäki, Taru; Sell, Anna

    2016-01-01

    Lasten osallistuminen kuntoutuksessa ei toteudu Suomessa riittävissä määrin. Opinnäytetyössä selvitettiin vastausta siihen, millaisia tekijöitä Lasten toimintamahdollisuuksien itsearvioinnissa (Child Occupational Self Assessment, COSA) on lapsen osallistumisen mahdollistamiseksi. Opinnäytetyön yhteistyökumppanina toimi Metropolia Ammattikorkeakoulun toimintaterapian tutkinto-ohjelma. Opinnäytetyön tavoitteena oli selvittää, miten COSA-itsearvioinnin avulla voidaan mahdollistaa lasten osallist...

  17. Defining the Content of an Online Sexual Health Intervention: The MenSS Website

    OpenAIRE

    Webster, R; Gerressu, M; Michie, S; Estcourt, C.; Anderson, J.; Ang, C S; Murray, E; Rait, G.; Stephenson, J; Bailey, J. V.

    2015-01-01

    BACKGROUND: Health promotion and risk reduction are essential components of sexual health care. However, it can be difficult to prioritize these within busy clinical services. Digital interventions may provide a new method for supporting these. OBJECTIVE: The MenSS (Men's Safer Sex) website is an interactive digital intervention developed by a multidisciplinary team, which aims to improve condom use in men who have sex with women (MSW). This paper describes the content of this intervention, a...

  18. Implementing OmpSs support for regions of data in architectures with multiple address spaces

    OpenAIRE

    Bueno J.; Martorell X; Badia R.M.; Ayguade E.; Labarta J.

    2013-01-01

    The need for features for managing complex data accesses in modern programming models has increased due to the emerging hardware architectures. HPC hardware has moved towards clusters of accelerators and/or multicores, architectures with a complex memory hierarchy exposed to the programmer. We present the implementation of data regions on the OmpSs programming model, a high-productivity annotation-based programming model derived from OpenMP. This enables the programmer to specify regions of s...

  19. Immune Responses of Specific-Pathogen-Free Mice to Chronic Helicobacter pylori (Strain SS1) Infection

    OpenAIRE

    Ferrero, Richard L.; Thiberge, Jean-Michel; Huerre, Michel; Labigne, Agnès

    1998-01-01

    A model permitting the establishment of persistent Helicobacter pylori infection in mice was recently described. To evaluate murine immune responses to H. pylori infection, specific-pathogen-free Swiss mice (n = 50) were intragastrically inoculated with 1.2 × 107 CFU of a mouse-adapted H. pylori isolate (strain SS1). Control animals (n = 10) received sterile broth medium alone. Animals were sacrificed at various times, from 3 days to 16 weeks postinoculation (p.i.). Quantitative culture of ga...

  20. Novel ssDNA viruses discovered in yellow-crowned parakeet (Cyanoramphus auriceps) nesting material.

    Science.gov (United States)

    Sikorski, Alyssa; Kearvell, Jonathan; Elkington, Simon; Dayaram, Anisha; Argüello-Astorga, Gerardo R; Varsani, Arvind

    2013-07-01

    During routine monitoring of yellow-crowned parakeets in the Poulter Valley of the South Island of New Zealand, a dead parakeet chick was discovered in a nest. Known parrot-infecting viruses, such as beak and feather disease virus (BFDV), avian polyomavirus (APV), and parrot hepatitis B virus (PHBV), were not detected in the nesting material. However, we recovered two novel single-stranded DNA viruses (ssDNA), CynNCXV (2308 nt) and CynNCKV (2087 nt), which have genome architectures similar to those of circoviruses, characterised by circular genomes with two large bidirectional open reading frames (ORFs). Both contain a stem-loop element with a conserved nonanucleotide motif, known to be required for rolling-circle replication. The full genomes had no BLASTn similarity to known ssDNA viruses. However, in both genomes the larger ORFs have BLAST similarity to known replication-associated proteins (Reps). CynNCKV has 30 % similarity to picobiliphyte nano-like virus (Picobiliphyte M5584-5) with 66-88 % coverage (e-value of 5×10(-33)), whereas CynNCXV has 33 % similarity to rodent stool-associated virus (RodSCV M-45) with 92-94 % coverage (e-value of 5 × 10(-31)). Found within these ORFs were the rolling-circle replication motifs I, II, III and the helicase motifs Walker A and Walker B. Maximum-likelihood phylogenetic analysis of the Reps reveals that these are two novel ssDNA viruses. At this point, we are unable to attribute the death of the parakeet to these two new novel ssDNA viruses. PMID:23417396

  1. Hei, mä osaan! : lasten visuomotoristen taitojen kehittymisen tukeminen toimintaterapiaryhmässä

    OpenAIRE

    Hokkanen, Teija; Riekki, Kristiina

    2006-01-01

    Tutkimustehtävänä oli selvittää tukiko Palokan terveysasemalla toteutettu toimintaterapiaryhmä lasten visuomotoristen taitojen kehitystä. Tutkimuksen tavoitteena oli myös selvittää seikkailun käytön mahdollisuuksia toimintaterapiaryhmässä. Tutkimus toteutettiin osana Palokan terveydenhuollon toimintaterapiapalveluja. Tutkimuksessa ohjattiin kuuden esikouluikäisen lapsen toimintaterapiaryhmää, jonka tavoitteena oli tukea visuomotoristen taitojen kehitystä kouluvalmiuksia ajatellen. Visuomotori...

  2. A Neural Network Approach to Blind Estimation of PN Spreading Sequence in DS/SS Signals

    Institute of Scientific and Technical Information of China (English)

    ZHANG Tian-qi; ZHOU Zheng-zhong

    2004-01-01

    In this paper, a new approach is proposed to estimate pseudo noise(PN) sequence in the lower SNR DS/SS signals blindly. This method utilizes the characteristics of self-organization, principal components analysis and extraction of unsupervised neural networks adequately, in addition to its higher-speed operation ability, successfully solve the difficult problem about PN sequence blind estimation. The theoretic analysis and experimental results show that this approach can work very well on lower SNR input signals.

  3. Evidence for 4-chlorobenzoic acid dehalogenation mediated by plasmids related to pSS50.

    OpenAIRE

    Layton, A C; Sanseverino, J; Wallace, W; Corcoran, C; Sayler, G. S.

    1992-01-01

    The biodegradation of 4-chlorobiphenyl usually proceeds through the intermediate 4-chlorobenzoate. Few bacterial strains can degrade 4-chlorobiphenyl to 4-chlorobenzoate and 4-chlorobenzoate to CO2. This study demonstrates that the 4-chlorobiphenyl-degrading Alcaligenes sp. strain ALP83 can degrade 4-chlorobenzoate to 4-hydroxybenzoate. The dehalogenase activity is correlated with a 10-kb fragment carried on plasmid pSS70.

  4. Dramatic improvement of anti-SS-A/Ro-associated interstitial lung disease after immunosuppressive treatment.

    Science.gov (United States)

    Paola, Caramaschi; Giuliana, Festi; Giovanni, Orsolini; Cristian, Caimmi; Domenico, Biasi

    2016-07-01

    The aim of the study was to report three patients affected by interstitial lung disease associated with positive anti-SS-A/Ro autoantibody who showed a dramatic improvement after immunosuppressive treatment. Medical charts were reviewed to obtain clinical data, laboratory parameters, lung function tests, high-resolution computed tomography results and response to immunosuppressive treatment. The three patients showed a clinical picture of a lung-dominant connective tissue disease characterized by a sudden onset with dyspnea, cough and subtle extrathoracic features together with positive anti-SS-A/Ro antibody and weak titer antinuclear antibodies. All three patients responded favorably to immunosuppressive therapy: Two cases were treated with a combination of corticosteroid and cyclophosphamide followed by mycophenolate mofetil; in the third patient, clinical benefit was obtained after rituximab was added to corticosteroid and immunosuppressant drug. In spite of an abrupt onset with significant lung function impairment, all three patients had a favorable clinical response to immunosuppressive therapy. This report may be useful in making therapeutic decisions in case of interstitial lung disease associated with anti-SS-A antibody.

  5. Exploring optimization parameters to increase ssDNA recombineering in Lactococcus lactis and Lactobacillus reuteri.

    Science.gov (United States)

    Van Pijkeren, Jan-Peter; Neoh, Kar Mun; Sirias, Denise; Findley, Anthony S; Britton, Robert A

    2012-01-01

    Single-stranded DNA (ssDNA) recombineering is a technology which is used to make subtle changes in the chromosome of several bacterial genera. Cells which express a single-stranded DNA binding protein (RecT or Bet) are transformed with an oligonucleotide which is incorporated via an annealing and replication-dependent mechanism. By in silico analysis we identified ssDNA binding protein homologs in the genus Lactobacillus and Lactococcus lactis. To assess whether we could further improve the recombineering efficiency in Lactobacillus reuteri ATCC PTA 6475 we expressed several RecT homologs in this strain. RecT derived from Enterococcus faecalis CRMEN 19 yielded comparable efficiencies compared with a native RecT protein, but none of the other proteins further increased the recombineering efficiency. We successfully improved recombineering efficiency 10-fold in L. lactis by increasing oligonucleotide concentration combined with the use of oligonucleotides containing phosphorothioate-linkages (PTOs). Surprisingly, neither increased oligonucleotide concentration nor PTO linkages enhanced recombineering in L. reuteri 6475. To emphasize the utility of this technology in improving probiotic features we modified six bases in a transcriptional regulatory element region of the pdu-operon of L. reuteri 6475, yielding a 3-fold increase in the production of the antimicrobial compound reuterin. Directed genetic modification of lactic acid bacteria through ssDNA recombineering will simplify strain improvement in a way that, when mutating a single base, is genetically indistinguishable from strains obtained through directed evolution. PMID:22750793

  6. DETECTION OF PERSISTENT GAMMA-RAY EMISSION TOWARD SS433/W50

    Energy Technology Data Exchange (ETDEWEB)

    Bordas, P.; Yang, R.; Kafexhiu, E.; Aharonian, F., E-mail: pol.bordas@mpi-hd.mpg.de, E-mail: ryang@mpi-hd.mpg.de [Max-planck-Institut für Kernphysik, Saupfercheckweg 1, D-69117 Heidelberg (Germany)

    2015-07-01

    The microquasar SS433 features the most energetic jets known in our Galaxy. A large fraction of the jet kinetic power is delivered to the surrounding W50 nebula at the jet termination shock, from which high-energy emission and cosmic-ray production have been anticipated. Here, we report on the detection of a persistent gamma-ray signal obtained with the Fermi Large Area Telescope from an unidentified source that we tentatively associate, given its 99.9% confidence level position accuracy and the lack of any other high-energy emitter counterpart in the studied region, with SS433. The obtained spectral energy distribution displays a distinct maximum at ∼250 MeV and only extends up to ∼800 MeV. We discuss the possibility that the observed gamma-ray emission is produced through proton–proton collisions at the SS433/W50 interaction regions. If the same mechanism is operating in other baryon-loaded microquasar jets, their collective contribution could represent a significant fraction of the total galactic cosmic-ray flux at GeV energies.

  7. When Microquasar Jets and Supernova Collide: Hydrodynamically Simulating the SS433-W50 Interaction

    CERN Document Server

    Goodall, Paul T; Blundell, Katherine M

    2011-01-01

    We present investigations of the interaction between the relativistic, precessing jets of the microquasar SS433 with the surrounding, expanding Supernova Remnant (SNR) shell W50, and the consequent evolution in the inhomogeneous Interstellar Medium (ISM). We model their evolution using the hydrodynamic FLASH code, which uses adaptive mesh refinement. We show that the peculiar morphology of the entire nebula can be reproduced to a good approximation, due to the combined effects of: (i) the evolution of the SNR shell from the free-expansion phase through the Sedov blast wave in an exponential density profile from the Milky Way disc, and (ii) the subsequent interaction of the relativistic, precessing jets of SS 433. Our simulations reveal: (1) Independent measurement of the Galaxy scale-height and density local to SS433 (as n_0 = 0.2 cm^{-3}, Z_d = 40 pc), with this scale-height being in excellent agreement with the work of Dehnen & Binney. (2) A new mechanism for hydrodynamic refocusing of conical jets. (3)...

  8. Electrical Properties of Al, Ag, Cu, Ti and SS Thin Film for Electrode of Solar Cell

    International Nuclear Information System (INIS)

    The Al, Ag, Cu, Ti and SS materials were deposited on the surface of glass substrate using plasma DC sputtering technique. The deposition process was done with the following plasma parameters : deposition time, gas pressure and substrate temperature with the aim to obtain a good conductance of thin films. Variation of substrate deposition time was 1 - 15 minutes, gas pressure was 5x10-2 - 7x10-2 torr and of temperature was 100 - 300 oC. The resistance measurement has been done by four points probes and the conductivity was calculated using mathematic formulation. It was obtained that the minimum resistance in the order of R = 0.07 Ω, was found at Ag materials and this was obtained at the following plasma parameters : deposition time 15 minutes, gas pressure 6x10-2 torr and temperature 300 oC, while, the resistance of : Cu, Al, Ti and SS materials were R = 0.13 Ω, R = 450 Ω, R = 633 Ω, R = 911 Ω respectively, It could be concluded that the Ag thin film has a minimum resistance, high conductivity compared to the other materials Al, Cu, Ti and SS. Ag is therefore the suitable material for applying as electrode of solar cell. (author)

  9. NTT-ME公司购买SS7产品武装自己

    Institute of Scientific and Technical Information of China (English)

    宣正元

    2001-01-01

    作为在全球电信网络中应用最为广泛的协议,SS7协议可以提供呼叫控制的功能并支持智能网(IN)应用。通信业中不断增加的新的分组交换解决方案,如VOIP、语音门户和无线IP网关,都依赖这个国际标准协议实现与现有电话网络的无缝连接。无论信号来自于IP网络还是PsTN,SS7都能快速和可靠地建立呼叫。随着电信运营商之间竞争的日趋激烈,很多公司结合开放系统并利用广泛使用的硬软件,通过SS7和智能网技术来提供新型服务,以超出竞争对手,获得新的利润。

  10. A novel, highly divergent ssDNA virus identified in Brazil infecting apple, pear and grapevine.

    Science.gov (United States)

    Basso, Marcos Fernando; da Silva, José Cleydson Ferreira; Fajardo, Thor Vinícius Martins; Fontes, Elizabeth Pacheco Batista; Zerbini, Francisco Murilo

    2015-12-01

    Fruit trees of temperate and tropical climates are of great economical importance worldwide and several viruses have been reported affecting their productivity and longevity. Fruit trees of different Brazilian regions displaying virus-like symptoms were evaluated for infection by circular DNA viruses. Seventy-four fruit trees were sampled and a novel, highly divergent, monopartite circular ssDNA virus was cloned from apple, pear and grapevine trees. Forty-five complete viral genomes were sequenced, with a size of approx. 3.4 kb and organized into five ORFs. Deduced amino acid sequences showed identities in the range of 38% with unclassified circular ssDNA viruses, nanoviruses and alphasatellites (putative Replication-associated protein, Rep), and begomo-, curto- and mastreviruses (putative coat protein, CP, and movement protein, MP). A large intergenic region contains a short palindromic sequence capable of forming a hairpin-like structure with the loop sequence TAGTATTAC, identical to the conserved nonanucleotide of circoviruses, nanoviruses and alphasatellites. Recombination events were not detected and phylogenetic analysis showed a relationship with circo-, nano- and geminiviruses. PCR confirmed the presence of this novel ssDNA virus in field plants. Infectivity tests using the cloned viral genome confirmed its ability to infect apple and pear tree seedlings, but not Nicotiana benthamiana. The name "Temperate fruit decay-associated virus" (TFDaV) is proposed for this novel virus. PMID:26186890

  11. An MC-SS Platform for Short-Range Communications in the Personal Network Context

    Directory of Open Access Journals (Sweden)

    Bakirtzoglou Zeta

    2008-01-01

    Full Text Available Abstract Wireless personal area networks (WPANs have gained interest in the last few years, and several air interfaces have been proposed to cover WPAN applications. A multicarrier spread spectrum (MC-SS air interface specified to achieve 130 Mbps in typical WPAN channels is presented in this paper. It operates in the 5.2 GHz ISM band and achieves a spectral efficiency of 3.25  . Besides the robustness of the MC-SS approach, this air interface yields to reasonable implementation complexity. This paper focuses on the hardware design and prototype of this MC-SS air interface. The prototype includes RF, baseband, and IEEE802.15.3 compliant medium access control (MAC features. Implementation aspects are carefully analyzed for each part of the prototype, and key hardware design issues and solutions are presented. Hardware complexity and implementation loss are compared to theoretical expectations, as well as flexibility is discussed. Measurement results are provided for a real condition of operations.

  12. An MC-SS Platform for Short-Range Communications in the Personal Network Context

    Directory of Open Access Journals (Sweden)

    Dominique Noguet

    2008-03-01

    Full Text Available Wireless personal area networks (WPANs have gained interest in the last few years, and several air interfaces have been proposed to cover WPAN applications. A multicarrier spread spectrum (MC-SS air interface specified to achieve 130 Mbps in typical WPAN channels is presented in this paper. It operates in the 5.2 GHz ISM band and achieves a spectral efficiency of 3.25 b·s−1·Hz−1. Besides the robustness of the MC-SS approach, this air interface yields to reasonable implementation complexity. This paper focuses on the hardware design and prototype of this MC-SS air interface. The prototype includes RF, baseband, and IEEE802.15.3 compliant medium access control (MAC features. Implementation aspects are carefully analyzed for each part of the prototype, and key hardware design issues and solutions are presented. Hardware complexity and implementation loss are compared to theoretical expectations, as well as flexibility is discussed. Measurement results are provided for a real condition of operations.

  13. Observation of white spot lesions using swept source optical coherence tomography (SS-OCT): in vitro and in vivo study.

    Science.gov (United States)

    Ibusuki, Takahide; Kitasako, Yuichi; Sadr, Alireza; Shimada, Yasushi; Sumi, Yasunori; Tagami, Junji

    2015-01-01

    This study aimed to assess swept source optical coherence tomography (SS-OCT) for in vitro and in vivo detection of enamel white spot lesion (WSL). WSLs without surface breakdown on 33 extracted human posterior teeth were non-invasively scanned using SSOCT. The teeth were then cross-sectioned and imaged under confocal laser scanning microscope (CLSM) and light microscopy (LM). SS-OCT cross-sectional images were compared with CLSM and LM. WSL shapes in SS-OCT images closely corresponded to those of LM. There were significant correlations (pSS-OCT and LM (r=0.92), SS-OCT and CLSM (r=0.80) and CLSM and LM (r=0.85). Six WSLs were also evaluated clinically using SS-OCT; clear in-depth images of these natural WSLs were obtained in vivo. SS-OCT appears to be an effective tool for observation of the internal structure of WSLs, enabling quantitative assessment of WSL depth. Such data can be considered in the clinical management of WSLs.

  14. Salmonella Typhimurium utilizes a T6SS-mediated antibacterial weapon to establish in the host gut.

    Science.gov (United States)

    Sana, Thibault G; Flaugnatti, Nicolas; Lugo, Kyler A; Lam, Lilian H; Jacobson, Amanda; Baylot, Virginie; Durand, Eric; Journet, Laure; Cascales, Eric; Monack, Denise M

    2016-08-23

    The mammalian gastrointestinal tract is colonized by a high-density polymicrobial community where bacteria compete for niches and resources. One key competition strategy includes cell contact-dependent mechanisms of interbacterial antagonism, such as the type VI secretion system (T6SS), a multiprotein needle-like apparatus that injects effector proteins into prokaryotic and/or eukaryotic target cells. However, the contribution of T6SS antibacterial activity during pathogen invasion of the gut has not been demonstrated. We report that successful establishment in the gut by the enteropathogenic bacterium Salmonella enterica serovar Typhimurium requires a T6SS encoded within Salmonella pathogenicity island-6 (SPI-6). In an in vitro setting, we demonstrate that bile salts increase SPI-6 antibacterial activity and that S Typhimurium kills commensal bacteria in a T6SS-dependent manner. Furthermore, we provide evidence that one of the two T6SS nanotube subunits, Hcp1, is required for killing Klebsiella oxytoca in vitro and that this activity is mediated by the specific interaction of Hcp1 with the antibacterial amidase Tae4. Finally, we show that K. oxytoca is killed in the host gut in an Hcp1-dependent manner and that the T6SS antibacterial activity is essential for Salmonella to establish infection within the host gut. Our findings provide an example of pathogen T6SS-dependent killing of commensal bacteria as a mechanism to successfully colonize the host gut. PMID:27503894

  15. Characterization of mixed monolayers of phosphatidylcholine and a dicationic gemini surfactant SS-1 with a langmuir balance: effects of DNA.

    Science.gov (United States)

    Matti, V; Säily, J; Ryhänen, S J; Holopainen, J M; Borocci, S; Mancini, G; Kinnunen, P K

    2001-01-01

    Monolayers of a cationic gemini surfactant, 2,3-dimethoxy-1,4-bis(N-hexadecyl-N;N-dimethyl-ammonium)butane dibromide (abbreviated as SS-1) and its mixtures with 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) were studied using a Langmuir balance. More specifically, we measured the force-area (pi-A) curves and determined the elastic area compressibility modulus (C) as a function of lateral packing pressure and the mole fraction of the cationic lipid (X(SS-1)), with and without DNA in the subphase. Both SS-1 and POPC exhibited smooth compression isotherms, indicating their monolayers to be in the liquid expanded state. Even low contents (X(SS-1) 0.5. At a higher concentration of DNA (1.88 microM base pairs) in the subphase corresponding to DNA/SS-1 charge ratio of 3.75 at X(SS-1) = 0.5, condensation was observed at all values of X(SS-1). PMID:11566784

  16. Synthesis and Characterization of PEDOT:P(SS-co-VTMS with Hydrophobic Properties and Excellent Thermal Stability

    Directory of Open Access Journals (Sweden)

    Wonseok Cho

    2016-05-01

    Full Text Available Hydrophobic and comparatively thermally-stable poly(3,4-ethylenedioxythiophene, i.e., poly(styrene sulfonate-co-vinyltrimethoxysilane (PEDOT:P(SS-co-VTMS copolymer was successfully synthesized via the introduction of silane coupling agent into the PSS main chain to form P(SS-co-VTMS copolymers. PSS and P(SS-co-VMTS copolymers were successfully synthesized via radical solution polymerization, and PEDOT:P(SS-co-VTMS was synthesized via Fe+-catalyzed oxidative polymerization. The characterization of PEDOT:P(SS-co-VTMS was performed through an analysis of Fourier transform infrared spectroscopy (FTIR results, water contact angle and optical images. The electrical properties of conductive PEDOT:P(SS-co-VTMS thin films were evaluated by studying the influence of the VTMS content on the electrical and physical properties. The conductivity of PEDOT:P(SS-co-VTMS decreased with an increase in the VTMS content, but was close to that of the PEDOT:PSS, 235.9 S·cm−1. The introduction of VTMS into the PSS copolymer improved the mechanical properties and thermal stability and increased the hydrophobicity. The thermal stability test at a temperature over 240 °C indicated that the sheet resistance of PEDOT:PSS increased by 3,012%. The sheet resistance of PEDOT:P(SS-co-VTMS, on the other hand, only increased by 480%. The stability of PEDOT:P(SS-co-VTMS was six-times higher than that of the reference PEDOT:PSS.

  17. Performance analysis and maintenance of SS150F type smoke machine%SS150F型烟雾机性能分析与维护保养

    Institute of Scientific and Technical Information of China (English)

    李传军; 滕越; 樊伟; 孙文明

    2013-01-01

    让部队卫生防疫人员了解和使用好SS150F型烟雾机.从卫生装备原理入手,结合操作规程和自身的维修经验进行分析.通过分析,取得了一些操作和维护经验,便于部队卫生防疫人员更好地使用卫生装备.对于进口的部队卫生装备,进行操作和原理分析,确保卫生装备的正确使用,延长使用寿命,充分发挥卫生装备的使用价值.

  18. Study on cracks of axle-hang box for SS7 locomotive%SS7机车抱轴箱体裂纹研究

    Institute of Scientific and Technical Information of China (English)

    张运伟; 封全保; 杨俊杰

    2005-01-01

    介绍抱轴箱体的结构特征和运行情况.利用有限元法对SS7型电力机车抱轴箱体3种结构的静强度做对比分析,证实了局部几何性应力集中对过早裂损的影响;利用电镜扫描技术对裂纹进行分析,结果表明抱轴箱体的裂纹断口特征相近,裂纹性质属于多源性低周疲劳裂纹,铸件中的夹杂、气孔等内部缺陷是导致过早裂损的主要原因.

  19. GSM SiNYALLERİNİN .IP OMURGASI İİZERiNI>E TAŞINMASI (SS7 OVER IP- NettcSS7

    Directory of Open Access Journals (Sweden)

    Çağatay Neftali Tülü

    2003-08-01

    Full Text Available Bu çalışmada günümüzün en popüler nıobilhaberleşme sistemi olan GSM [1 l sisteminin, kendiöğeleri aras1ndaki en temel sinyalleşme sistemi olannumara 7 (SS7 [2 sinyallerinin, günümüzün enyaygın iletim omurgast olan ve tüm dünyadakibilgisayarları birbirine bağlayan IP (3] omurgasıüzerinden taşınabilmesi için gerekli si stemin tasar11n1ve gerçeklenınesi üzerinde çahş1lmtştır. Klasil\\ SS7iletimine göre en büyüllt; faydası çok düşül{ maliyetiolan bu sistem sayesinde, dünyadaki b i r çok GSMşebekesi ara bağlanti harcanıalarıııda büyükmiktarda tasarrufa gideceklerdir. Aralarındakiklasik 64 KB/sn 'lik ara bağlantı yerine, bununüzerinde bir bağlantı hızı ile iki şebeke haberleşmişolacaktır. Bu değişimierin etkisi abonelere fiyatlardadüşüş ve servis kalitesinde yükseliş olarakyansıyacaktır.

  20. Synthesis of Novel High Energy Polyaniline Derivative Containing S-S Bond in Six-membered Cycle

    Institute of Scientific and Technical Information of China (English)

    ZHOU Lei; DENG Shi-Ren; LI Zao-Ying

    2003-01-01

    @@ A novel conducting polymer as new class of high energy storage material, [1,2] which has one six-membered cycle containing S-S bond in the moiety of aniline was synthesized. The polymer had enhanced redox progress due to an intramolecular electrocatalytic effect of polyaniline on S-S bond. The polymer has some advantages because of its high theoretical specific energy and fast kinetics when used in lithium batteries. The most promised advantage of it is that the intramolecular cleavage and recombination of the S-S bond improve the cyclicability greatly.

  1. Virtualisointityökalujen käyttökelpoisuuden analysointi vaativassa palvelinympäristössä

    OpenAIRE

    Chistyakov, Konstantin

    2010-01-01

    Täma insinöörityö tehtiin Keypro Oy:n Vantaan yksikölle. Työssä selvitettiin, miten avoimen lähdekoodin Kernel based Virtual Machine (KVM) -virtualisointitekniikkaa voidaan hyödyntää yrityksen palvelinympäristössä. Työ aloitettiin perehtymällä virtualisointitekniikoihin ja KVM-hypervisorin arkkitehtuuriin. Sen jälkeen asennettiin KVM-hypervisori Ubuntu Server 9.10 -käyttöjärjestelmään ja tutkittiin sen ominaisuuksia. Työssä testattiin erilaiset asennus- ja konfigurointivaihtoehdot...

  2. Porkkanapesätoiminta lasten kokemana : "Porkkanapesässä on kivaa, kun saa olla kaikkien kanssa"

    OpenAIRE

    Painokallio, Sanna; Viitanen, Leena

    2006-01-01

    Opinnäytetyössä kartoitettiin Mannerheimin Lastensuojeluliiton Satakunnan piirin järjestämän porkkanapesätoiminnan merkitystä ja roolia Pormestarinluodon lasten elämässä. Tutkimuksessa huomioitiin myös lasten perheiden näkökulmat Porkkanapesän tarjoamasta toiminnasta. Tutkimus tehtiin yhteistyössä Mannerheimin Lastensuojeluliiton kanssa. Tavoitteena oli hankkia porkkanapesätoiminnasta ajantasaista tietoa ja huomioida erityisesti lasten mielipiteet. Tutkimusmenetelmänä käytettiin kvalitatiivis...

  3. 78 FR 47056 - Proposed Collection; Comment Request for Forms 941, 941-PR, 941-SS, 941-X, 941-X(PR), Schedule B...

    Science.gov (United States)

    2013-08-02

    ... Internal Revenue Service Proposed Collection; Comment Request for Forms 941, 941-PR, 941- SS, 941-X, 941-X... Federal Tax Return), 941-PR, 941-SS (Employer's Quarterly Federal Tax Return-American Samoa, Guam, the... Quarterly Federal Tax Return. OMB Number: 1545-0029. Form Numbers: 941, 941-PR, 941-SS, 941-X,...

  4. 75 FR 51531 - Proposed Collection; Comment Request for Forms 941, 941-PR, 941-SS, 941-X, 941-X(PR), Schedule B...

    Science.gov (United States)

    2010-08-20

    ... Internal Revenue Service Proposed Collection; Comment Request for Forms 941, 941-PR, 941- SS, 941-X, 941-X... Seguro Social Y Al Seguro Medicare), 941-SS (Employer's Quarterly Federal Tax Return-American Samoa, Guam... Number: 1545-0029. Form Numbers: 941, 941-PR, 941-SS, 941-X, 941-X(PR), Schedule R (Form 941), Schedule...

  5. SS7型电力机车轮缘偏磨问题的探讨%Probe into bias-abrasion of wheel flange of SS7 electric locomotive

    Institute of Scientific and Technical Information of China (English)

    王志平

    2005-01-01

    针对SS7型电力机车轮缘偏磨的问题,结合机车轮缘磨耗的机理及SS7型电力机车设计的结构特点从理论上进行了分析,提出了相应的减缓轮缘偏磨的建议及措施.

  6. Binding of antibodies to the extractable nuclear antigens SS-A/Ro and SS-B/La is induced on the surface of human keratinocytes by ultraviolet light (UVL): Implications for the pathogenesis of photosensitive cutaneous lupus

    Energy Technology Data Exchange (ETDEWEB)

    Furukawa, F.; Kashihara-Sawami, M.; Lyons, M.B.; Norris, D.A. (Univ. of Colorado School of Medicine, Denver (USA))

    1990-01-01

    Autoantibodies to the non-histone nucleoprotein antigens SS-A/Ro, SS-B/La, and RNP are highly associated with photosensitive cutaneous lupus erythematosus (LE). In order to better understand the potential mechanisms of ultraviolet (UV) light on photosensitivity in patients with cutaneous LE, we designed immunopathologic in vitro and in vivo experiments to evaluate the effects of UV on the binding of such autoantibodies to the surface of human keratinocytes, one major target of immunologic damage in photosensitive LE. Short-term 2% paraformaldehyde fixation of suspensions of cultured human keratinocytes previously incubated with monospecific antiserum probes enabled the detection of ENA expression on the cell surface by flow-cytometry analysis. UVB light (280-320 nm) induced the binding of monospecific antibody probes for SS-A/Ro and SS-B/La on keratinocytes in a dose-dependent pattern with maximal induction observed at the dose of 200 mJ/cm2 UVB. Binding of SS-A/Ro, SS-B/La, and RNP antibody was augmented strongly, but binding of anti-Sm was very weak. In contrast, UVA (320-400 nm) light had no effect on the induction of binding of these antibody probes. Identical results were seen by standard immunofluorescence techniques. Hydroxyurea-treated keratinocytes showed similar induction of those antigens by UVB irradiation, which suggested that ENA expression on cultured keratinocytes by UVB were cell-cycle independent. Tunicamycin, an inhibitor of glycosylation of proteins, reduced UVB light effect on the SS-A/Ro and SS-B/La antigen's expression. These in vitro FACS analyses revealed that ENA augmentation on the keratinocyte cell surface was dose dependent, UVB dependent, glycosylation dependent, and cell-cycle independent. In vivo ENA augmentation on the keratinocyte surface was examined in suction blister epidermal roofs.

  7. Työntekijän oikeus yksityisyyteen työelämässä

    OpenAIRE

    Vepsäläinen, Ninja

    2010-01-01

    Tämän opinnäytetyön tavoite oli antaa kattava selvitys työntekijän oikeudesta yksityisyyteen työelämässä sekä arvioida sitä, toteutuuko työntekijän oikeus yksityisyyteen työelämässä. Työni perustuu työntekijän oikeudesta yksityisyyden suojaan työelämässä annettuun lakiin, henkilötietolakiin ja yhteistoimintalakiin. Käsittelen työssäni ajankohtaisia työntekijän yksityisyyteen liittyviä kysymyksiä, työntekijöistä kerättäviä henkilötietoja, työntekijöiden teknistä valvontaa, työantajan oikeutta ...

  8. Mechanical properties of Cu sbnd Cr sbnd Zr alloy and SS316 joints fabricated by friction welding method

    Science.gov (United States)

    Tsuchiya, Kunihiko; Kawamura, Hiroshi

    1996-10-01

    Copper alloys with high-strength and high-conductivity are being considered for several magnetic fusion energy applications such as the first wall in high power-density devices, resistive magnetic coils, and high-heat flux components. For example, the stainless steel is a structural material while Cu-alloy acts as a heat sink material for the surface heat flux in the first wall. Therefore, development of reliable joints between Cu-alloys and stainless steel (SS316) is required. In the present work, joining tests on Cu—1%Cr—1%Zr/SS316 by friction welding were performed, and optimum fabricating conditions of the Cu-alloy/SS316 joint were determined. Additionally, the characteristics of tensile strength, hardness, metallographical observation and SEM/EPMA analyses on Cu—1%Cr—1%Zr/SS316 fabricated by friction welding were evaluated.

  9. [Comparison of tests for SS-A/Ro, Ro52 and Ro60 in predicting congenital heart block].

    Science.gov (United States)

    Miyano, Akira; Nakayama, Masahiro; Waguri, Masako; Nakanishi, Isao

    2014-04-01

    Neonatal lupus erythematosus (NLE) is a rare syndrome caused by the transplacental passage of maternal autoantibodies. Anti SS-A antibodies of a mother with Sjögren syndrome are associated with congenital heart block (CHB) in the newborns with NLE. The purpose of this study was to investigate the utility of maternal antibody titers for SS-A, Ro52 and Ro60 in mothers of newborns with CHB. The study involved a total of 304 cases, 25 from mothers of newborns with CHB, 104 from mothers of newborns without and 175 from mothers suspected to have connective tissue diseases. All sera were tested with the EliA SS-A, EliA Ro52, EliA Ro60, MESACUP Ro52 and MESACUP Ro60. The concordance rate of Ro52 assays was 93.4%, whereas Ro60 assays showed a lower concordance rate (74.7%). The areas under the curve (AUC) of the EliA assays were higher than those of the MESACUP assays. The optimal cut-off values for EliA SS-A/Ro and EliA Ro60 as derived from the ROC analysis were 2027 U/mL and 2446 U/mL, respectively. The sensitivity and specificity for EliA SS-A using optimal cut-off values were 96.0% and 92.3%, respectively. A titer of 90% positive predictive value for EliA SS-A was reached at a cut-off of 9897.1 U/mL, corresponding to sensitivity and specificity values of 36.0% and 100%, respectively. In conclusion, the optimal cut-off value for EliA SS-A is likely to be useful for application in clinical practice for the EliA SS-A measurements in mothers to evaluate the risk of NLE for their newborns.

  10. Investigation on fracture behavior of the welded joint HAZ of ultra-fine grain steel SS400

    Institute of Scientific and Technical Information of China (English)

    朱政强; 陈立功; 荆洪阳; 葛景国; 倪纯珍; 饶德林

    2003-01-01

    The critical crack dimensions of both base-metal specimen and HAZ specimen are measured via wide-plate tensile tests. Based on the "fitness for purpose" principle, the fracture behavior of the ultra-fine grain steel SS400 welded joint HAZ is assessed. The test results indicate that overmatching is benefit for the whole capability's improvement of ultra-fine grain steel SS400. The test results are confirmed by using finite element method (FEM).

  11. The eSS rat, a nonobese model of disordered glucose and lipid metabolism and fatty liver

    OpenAIRE

    Daniele Stella M; Montenegro Silvana M.; Tarres María C; Picena Juan C; Martinez Stella M

    2010-01-01

    Abstract Background eSS is a rat model of type 2 diabetes characterized by fasting hyperglycemia, glucose intolerance, hyperinsulinemia and early hypertriglyceridemia. Diabetic symptoms worsen during the second year of life as insulin release decreases. In 12-month-old males a diffuse hepatic steatosis was detected. We report the disturbances of lipid metabolism of the model with regard to the diabetic syndrome. Methods The study was conducted in eight 12-month-old eSS male rats and seven age...

  12. Kouluikäisen seksuaaliterveyskasvatusmateriaali historiasta tulevaisuuteen : materiaalien käyttö terveydenhoitajan työssä

    OpenAIRE

    Paajanen, Heini; Perttula, Eliisa

    2015-01-01

    Tämä opinnäytetyö on osa Metropolian Ammattikorkeakoulun Hoitotyön koulutuksen museon hanketta. Hankeen tavoitteena on kuvata suomalaisen hoitotyön historiaa. Opinnäytetyömme aiheena on seksuaaliterveyskasvatus kouluissa historiasta nykyaikaan, erikoistarkastelussa seksuaaliterveyskasvatusmateriaalien käyttö kouluterveydenhoitajan työssä. Opinnäytetyö on toteutettu vuonna 2015. Opinnäytetyössä vastaamme siihen millaista seksuaalikasvatus ja seksuaalikasvatusmateriaalit olivat 1900-luvulta...

  13. Highlights from the Functional Single Nucleotide Polymorphisms Associated with Human Muscle Size and Strength or FAMuSS Study

    OpenAIRE

    Pescatello, Linda S.; Devaney, Joseph M.; Hubal, Monica J; Thompson, Paul D.; Hoffman, Eric P

    2013-01-01

    The purpose of the Functional Single Nucleotide Polymorphisms Associated with Human Muscle Size and Strength study or FAMuSS was to identify genetic factors that dictated the response of health-related fitness phenotypes to resistance exercise training (RT). The phenotypes examined were baseline muscle strength and muscle, fat, and bone volume and their response to RT. FAMuSS participants were 1300 young (24 years), healthy men (42%) and women (58%) that were primarily of European-American de...

  14. Dahl SS rats demonstrate enhanced aortic perivascular adipose tissue-mediated buffering of vasoconstriction through activation of NOS in the endothelium.

    Science.gov (United States)

    Spradley, Frank T; Ho, Dao H; Pollock, Jennifer S

    2016-02-01

    Perivascular adipose tissue (PVAT) mediates buffering of vasoconstriction through activation of endothelium-derived factors. We hypothesized that the PVAT of Dahl salt-sensitive (Dahl SS) rats has reduced ability to buffer vasoconstriction. Vascular reactivity experiments were performed on aortic rings with PVAT intact (+PVAT) or removed (-PVAT), and endothelium intact (+ENDO) or removed (-ENDO) from Dahl SS rats and control SS.13(BN) rats (Dahl SS rats that have had chromosome 13 completely replaced with that of the Brown Norway rat, rendering this strain insensitive to high-salt or high-fat diet-induced hypertension). Endothelial dysfunction, assessed by ACh-mediated vasorelaxation, was confirmed in aortic rings of Dahl SS rats. The +PVAT+ENDO aortic rings had indistinguishable phenylephrine-induced vasoconstriction between genotypes. In both strains, removal of PVAT significantly enhanced vasoconstriction. Dahl SS rat -PVAT+ENDO aortic rings displayed exaggerated vasoconstriction to phenylephrine vs. SS.13(BN) rats, indicating that PVAT-mediated buffering of vasoconstriction was greater in Dahl SS rats. Removal of both the ENDO and PVAT restored vasoconstriction in both strains. The nitric oxide synthase (NOS) inhibitor, N(ω)-nitro-L-arginine methyl ester (L-NAME), produced a similar effect as that seen with -ENDO. These data indicate that the function of the PVAT to activate endothelium-derived NOS is enhanced in Dahl SS compared with SS.13(BN) rats and, most likely, occurs through a pathway that is distinct from ACh-mediated activation of NOS. PVAT weight and total PVAT leptin levels were greater in Dahl SS rats. Leptin induced a significantly decreased vasoconstriction in -PVAT+ENDO aortic rings from Dahl SS rats, but not SS.13(BN) rats. In contrast to our initial hypothesis, PVAT in Dahl SS rats buffers vasoconstriction by activating endothelial NOS via mechanisms that may include the involvement of leptin. Thus, the PVAT serves a vasoprotective role in

  15. Developmental patterns of GHr and SS mRNA expression in porcine gastric tissue

    Institute of Scientific and Technical Information of China (English)

    Dong Xia; Ru-Qian Zhao; Xi-Hui Wei; Qing-Fu Xu; Jie Chen

    2003-01-01

    AIM: The present study was aimed to investigate the developmental patterns of growth hormone receptor (GHr)and somatostatin (SS) mRNA expression in porcine gastric tissue and its relationship with gastric growth and gastric functional development.METHODS: Erhualian and Large White boars were selected randomly and sampled at birth (DO), 3, 20, 30, 90, 120 and 180 days of age respectively, meanwhile the bodyweight and gastric weight were recorded. The single tube semiquantitative RT-PCR was applied in this experiment to investigate the developmental patterns of gastric GHr and SS mRNA expression, the correlations between the patterns of mRNA expression and the relative gastric weight (ratio of gastric weight to bodyweight) and the pepsin contents in gastric mucous membrane were analyzed. In order to further elucidate the effect of GH on gastric function, the primary cultures of gastric fundic mucosal cells were treated with 2ng/ml, 20 ng/ml and 200 ng/ml of rpGH for 18 hrs respectively,and the pepsin contents in culture medium were measured.RESULTS: The expression of GHr mRNA was high at birth,followed by a significant decrease at 3 days of age (D3) in both breeds. In Large White boars, the expression of GHr mRNA reached a peak at D90 and remained a plateau afterward. In Erhualian pigs, however, a slight yet significant increase occurred at D30 reaching a level that was kept constant thereafter. From birth to D30, the expression of GHr mRNA in gastric tissue was higher in Erhualian boars than that in Large White boars, but from D90 to D180, the higher expression of GHr mRNA was found in Large White boars.The gastric GHr mRNA expression was significantly correlated with the relative gastric weight (r=0.541, P<0.05) and pepsin content in gastric mucosa (r=0.625, P<0.05) respectively.The gastric SS mRNA expression was high at birth (Erhualian 1.59, Large White 0.80), but dropped significantly at D3 (Erhualian 0.95, Large White 0.19, P<0.05), a stepwise increase

  16. Noninvasive cross-sectional imaging of proximal caries using swept-source optical coherence tomography (SS-OCT) in vivo.

    Science.gov (United States)

    Shimada, Yasushi; Nakagawa, Hisaichi; Sadr, Alireza; Wada, Ikumi; Nakajima, Masatoshi; Nikaido, Toru; Otsuki, Masayuki; Tagami, Junji; Sumi, Yasunori

    2014-07-01

    The aim of this study was to determine the diagnostic accuracy of swept-source optical coherent tomography (SS-OCT) in detecting and estimating the depth of proximal caries in posterior teeth in vivo. SS-OCT images and bitewing radiographs were obtained from 86 proximal surfaces of 53 patients. Six examiners scored the locations according to a caries lesion depth scale (0-4) using SS-OCT and the radiographs. The results were compared with clinical observations obtained after the treatment. SS-OCT could detect the presence of proximal caries in tomograms that were synthesized based on the backscatter signal obtained from the proximal carious lesion through occlusal enamel. SS-OCT showed significantly higher sensitivity and larger area under the receiver operating characteristic curve than radiographs for the detection of cavitated enamel lesions and dentin caries (Student's t -test, p SS-OCT appears to be a more reliable and accurate method than bitewing radiographs for the detection and estimation of the depth of proximal lesions in the clinical environment.

  17. The simultaneous production of sphingan Ss and poly(R-3-hydroxybutyrate) in Sphingomonas sanxanigenens NX02.

    Science.gov (United States)

    Wu, Mengmeng; Li, Guoqiang; Huang, Haidong; Chen, Sibin; Luo, Ying; Zhang, Wenwen; Li, Keran; Zhou, Jiefang; Ma, Ting

    2016-01-01

    Sphingans and poly(R-3-hydroxybutyrate) (PHB) are both widely used biopolymers produced by bacteria. In the batch fermentation of Sphingomonas sanxanigenens NX02 in a 5L fermenter using glucose as carbon source, ivory colored sphingan Ss production was a growth-associated process with a maximum purified production of 14.88 ± 0.83 g/L, while 6.08 ± 0.23 g/L PHB was simultaneously produced. Sphingan Ss and PHB were separated by a simple dilution, heating and centrifugation or filtration process, and sphingan Ss can be cost-effectively extracted using a small amount of acid rather than multi-fold volumes of alcohols. From ultrathin sections of S. sanxanigenens NX02, we found that the interior space of the cells was filled with PHB granules, and the outside was surrounded by abundant Ss. The purified sphingan Ss can be used as an excellent gelling and emulsifying agent in biotechnology applications such as food, personal care and production processes. Proposed pathways of Ss and PHB biosynthesis from glucose are also presented.

  18. Mycophenolate mofetil prevents high-fat diet-induced hypertension and renal glomerular injury in Dahl SS rats.

    Science.gov (United States)

    Spradley, Frank T; De Miguel, Carmen; Hobbs, Janet; Pollock, David M; Pollock, Jennifer S

    2013-11-01

    We designed experiments to test the hypothesis that Dahl salt-sensitive (SS) rats are sensitive to high-fat diet (HFD)-induced hypertension and renal injury via an inflammatory mechanism. Twelve-week-old Dahl SS rats were maintained on a normal diet (ND; 14% fat), HFD (59% fat), or HFD supplemented with the lymphocyte immunosuppressive agent, mycophenolate mofetil (HFD + MMF; 30 mg/kg/day orally in diet), for a period of 4 weeks. Mean arterial pressure (MAP), metabolic parameters, T lymphocyte (CD3(+)) localization, and renal structural damage were assessed during the studies. Four weeks of HFD significantly elevated MAP and visceral adiposity without changing circulating levels of lipids or adipokines. Immunohistochemical analysis demonstrated that SS rats on HFD had significantly greater numbers of CD3(+) cells in renal glomerular and medullary areas compared to ND SS rats. Additionally, HFD led to increased glomerular injury, but did not alter renal medullary injury. Chronic MMF treatment in HFD-fed Dahl SS rats reduced MAP, visceral adiposity, infiltration of CD3(+) cells in the glomerulus, as well as glomerular injury. However, MMF treatment did not alter HFD-induced infiltration of CD3(+) cells in the renal medulla. In conclusion, Dahl SS rats are sensitized to HFD-induced hypertension and renal glomerular injury via infiltration of T lymphocytes.

  19. ssODN-mediated knock-in with CRISPR-Cas for large genomic regions in zygotes.

    Science.gov (United States)

    Yoshimi, Kazuto; Kunihiro, Yayoi; Kaneko, Takehito; Nagahora, Hitoshi; Voigt, Birger; Mashimo, Tomoji

    2016-01-01

    The CRISPR-Cas system is a powerful tool for generating genetically modified animals; however, targeted knock-in (KI) via homologous recombination remains difficult in zygotes. Here we show efficient gene KI in rats by combining CRISPR-Cas with single-stranded oligodeoxynucleotides (ssODNs). First, a 1-kb ssODN co-injected with guide RNA (gRNA) and Cas9 messenger RNA produce GFP-KI at the rat Thy1 locus. Then, two gRNAs with two 80-bp ssODNs direct efficient integration of a 5.5-kb CAG-GFP vector into the Rosa26 locus via ssODN-mediated end joining. This protocol also achieves KI of a 200-kb BAC containing the human SIRPA locus, concomitantly knocking out the rat Sirpa gene. Finally, three gRNAs and two ssODNs replace 58-kb of the rat Cyp2d cluster with a 6.2-kb human CYP2D6 gene. These ssODN-mediated KI protocols can be applied to any target site with any donor vector without the need to construct homology arms, thus simplifying genome engineering in living organisms. PMID:26786405

  20. Sequence-dependent electrical response of ssDNA-decorated carbon nanotube, field-effect transistors to dopamine

    Directory of Open Access Journals (Sweden)

    Hari Krishna Salila Vijayalal Mohan

    2014-11-01

    Full Text Available Single-walled carbon nanotube (SWCNT-based field-effect transistors (FETs have been explored for use as biological/chemical sensors. Dopamine (DA is a biomolecule with great clinical significance for disease diagnosis, however, SWCNT FETs lack responsivity and selectivity for its detection due to the presence of interfering compounds such as uric acid (UA. Surface modification of CNTs using single-stranded deoxyribonucleic acid (ssDNA renders the surface responsive to DA and screens the interferent. Due to the presence of different bases in ssDNA, it is necessary to investigate the effect of sequence on the FET-based molecular recognition of DA. SWCNT FETs were decorated with homo- and repeated-base ssDNA sequences, and the electrical response induced by DA in the presence and absence of UA was gauged in terms of the variation in transistor electrical parameters including conductance, transconductance, threshold voltage and hysteresis gap. Our results showed that the response of ssDNA-decorated devices to DA, irrespective of the presence or absence of UA, was DNA sequence dependent and exhibited the trend: G > A > C and GA > GT > AC > CT, for homo- and repeated-base sequences, respectively. The different response of various SWCNT–ssDNA systems to DA underlines the sequence selectivity, whereas the detection of DA in the presence of UA highlights the molecular selectivity of the ssDNA-decorated devices.

  1. Differential sensitivity of the nicotinic receptor of long (LS) and short (SS) sleep mice to ethanol (E) and forane (F)

    Energy Technology Data Exchange (ETDEWEB)

    McArdle, J.J.; Choi, J.J. (New Jersey Medical School, Newark (USA))

    1989-02-09

    Studies of inbred mice indicate that heredity determines the behavioral response to CNS depressants. For example, LS mice lose their righting reflex at blood levels of E having no effect on this reflex of SS mice. In order to determine if such differential sensitivity extends to the effects of depressants known to alter the mean open time (tau) of the ion channel activated by the nicotinic acetylcholine receptor (AR), we used an extracellular electrode to record miniature end-plate currents (23 C) from the triangularis sterni muscle of adult male LS and SS mice. The average decay time constant (tau) of 70 currents was calculated before, during and after drug exposure. Tau was the same for LS and SS mice (1.41 {plus minus} 0.03 mS and 1.47 {plus minus} 0.02 mS, respectively) prior to treatment and was reversible prolonged by E and shortened by F as expected. However, tau of SS mice was more responsive. For example, 25 mM of E increased tau by 12.9% and 3.8% in SS and LS mice, respectively. Likewise, the decrease of tau in response to 3 mM F was 18.5% and 9.2%. The net result was that the curve relating tau for LS mice to drug concentration was to the right of the for SS mice. These data suggest that the sensitivity of the peripheral AR to CNS depressants can be genetically controlled.

  2. The SsDREB Transcription Factor from the Succulent Halophyte Suaeda salsa Enhances Abiotic Stress Tolerance in Transgenic Tobacco

    Directory of Open Access Journals (Sweden)

    Xu Zhang

    2015-01-01

    Full Text Available Dehydration-responsive element-binding (DREB transcription factor (TF plays a key role for abiotic stress tolerance in plants. In this study, a novel cDNA encoding DREB transcription factor, designated SsDREB, was isolated from succulent halophyte Suaeda salsa. This protein was classified in the A-6 group of DREB subfamily based on multiple sequence alignments and phylogenetic characterization. Yeast one-hybrid assays showed that SsDREB protein specifically binds to the DRE sequence and could activate the expression of reporter genes in yeast, suggesting that the SsDREB protein was a CBF/DREB transcription factor. Real-time RT-PCR showed that SsDREB was significantly induced under salinity and drought stress. Overexpression of SsDREB cDNA in transgenic tobacco plants exhibited an improved salt and drought stress tolerance in comparison to the nontransformed controls. The transgenic plants revealed better growth, higher chlorophyll content, and net photosynthesis rate, as well as higher level of proline and soluble sugars. The semiquantitative PCR of transgenics showed higher expression of stress-responsive genes. These data suggest that the SsDREB transcription factor is involved in the regulation of salt stress tolerance in tobacco by the activation of different downstream gene expression.

  3. Rad52-mediated DNA annealing after Rad51-mediated DNA strand exchange promotes second ssDNA capture.

    Science.gov (United States)

    Sugiyama, Tomohiko; Kantake, Noriko; Wu, Yun; Kowalczykowski, Stephen C

    2006-11-29

    Rad51, Rad52, and RPA play central roles in homologous DNA recombination. Rad51 mediates DNA strand exchange, a key reaction in DNA recombination. Rad52 has two distinct activities: to recruit Rad51 onto single-strand (ss)DNA that is complexed with the ssDNA-binding protein, RPA, and to anneal complementary ssDNA complexed with RPA. Here, we report that Rad52 promotes annealing of the ssDNA strand that is displaced by DNA strand exchange by Rad51 and RPA, to a second ssDNA strand. An RPA that is recombination-deficient (RPA(rfa1-t11)) failed to support annealing, explaining its in vivo phenotype. Escherichia coli RecO and SSB proteins, which are functional homologues of Rad52 and RPA, also facilitated the same reaction, demonstrating its conserved nature. We also demonstrate that the two activities of Rad52, recruiting Rad51 and annealing DNA, are coordinated in DNA strand exchange and second ssDNA capture. PMID:17093500

  4. Microstructural studies of suck cast (Zr-SS)-3 and 5 AI alloys for nuclear metallic waste form

    International Nuclear Information System (INIS)

    Management of radioactive metallic waste using 'alloy melting route' is currently being investigated. For disposal of Zr and SS base nuclear metallic wastes, Zr-stainless steel (SS) hybrid alloys are being considered as baseline alloys for developing metallic-waste-form (MWF) alloys. In this context Zr-16 wt. %55 has been selected for MWF alloy in our previous study. In present study, to include amorphous phase in this alloy, 3 and 5 wt. % Al has been added in order to improve desirable properties and useful features of MWF and the two alloys have been prepared by suck casting techniques. Microstructure of these alloys have been investigated by optical and electron microscopy which shows occurrence of two different phases, e.g. dark grey and white phases, in (Zr-16 SS)-3 Al and three different phases, e.g. grey, dark grey and white phases in (Zr-16 SS)-5 AI. Electron diffraction and X-ray diffraction (XRD) analyses of these two alloy specimens revealed the occurrence of Zr (Fe, Cr, AI) (dark grey) and Zr2 (Fe, Cr, AI) (white) phases in (Zr-16 SS)-3 Al whereas, Zr (Fe, Cr, AI) (dark grey), Zr2 (Fe, Cr, AI) (grey) and Zr3(Fe, Cr, AI) (white) phases were found in (Zr-16 SS)-5 AI. In addition, presence of amorphous phase was indicated by XRD analysis that could be confirmed by transmission electron microscopy of these two alloys. (author)

  5. The SsDREB Transcription Factor from the Succulent Halophyte Suaeda salsa Enhances Abiotic Stress Tolerance in Transgenic Tobacco.

    Science.gov (United States)

    Zhang, Xu; Liu, Xiaoxue; Wu, Lei; Yu, Guihong; Wang, Xiue; Ma, Hongxiang

    2015-01-01

    Dehydration-responsive element-binding (DREB) transcription factor (TF) plays a key role for abiotic stress tolerance in plants. In this study, a novel cDNA encoding DREB transcription factor, designated SsDREB, was isolated from succulent halophyte Suaeda salsa. This protein was classified in the A-6 group of DREB subfamily based on multiple sequence alignments and phylogenetic characterization. Yeast one-hybrid assays showed that SsDREB protein specifically binds to the DRE sequence and could activate the expression of reporter genes in yeast, suggesting that the SsDREB protein was a CBF/DREB transcription factor. Real-time RT-PCR showed that SsDREB was significantly induced under salinity and drought stress. Overexpression of SsDREB cDNA in transgenic tobacco plants exhibited an improved salt and drought stress tolerance in comparison to the nontransformed controls. The transgenic plants revealed better growth, higher chlorophyll content, and net photosynthesis rate, as well as higher level of proline and soluble sugars. The semiquantitative PCR of transgenics showed higher expression of stress-responsive genes. These data suggest that the SsDREB transcription factor is involved in the regulation of salt stress tolerance in tobacco by the activation of different downstream gene expression. PMID:26504772

  6. Thick SS316 materials TIG welding development activities towards advanced fusion reactor vacuum vessel applications

    Science.gov (United States)

    Kumar, B. Ramesh; Gangradey, R.

    2012-11-01

    Advanced fusion reactors like ITER and up coming Indian DEMO devices are having challenges in terms of their materials design and fabrication procedures. The operation of these devices is having various loads like structural, thermo-mechanical and neutron irradiation effects on major systems like vacuum vessel, divertor, magnets and blanket modules. The concept of double wall vacuum vessel (VV) is proposed in view of protecting of major reactor subsystems like super conducting magnets, diagnostic systems and other critical components from high energy 14 MeV neutrons generated from fusion plasma produced by D-T reactions. The double walled vacuum vessel is used in combination with pressurized water circulation and some special grade borated steel blocks to shield these high energy neutrons effectively. The fabrication of sub components in VV are mainly used with high thickness SS materials in range of 20 mm- 60 mm of various grades based on the required protocols. The structural components of double wall vacuum vessel uses various parts like shields, ribs, shells and diagnostic vacuum ports. These components are to be developed with various welding techniques like TIG welding, Narrow gap TIG welding, Laser welding, Hybrid TIG laser welding, Electron beam welding based on requirement. In the present paper the samples of 20 mm and 40 mm thick SS 316 materials are developed with TIG welding process and their mechanical properties characterization with Tensile, Bend tests and Impact tests are carried out. In addition Vickers hardness tests and microstructural properties of Base metal, Heat Affected Zone (HAZ) and Weld Zone are done. TIG welding application with high thick SS materials in connection with vacuum vessel requirements and involved criticalities towards welding process are highlighted.

  7. Initial experiences with RoSS surgical simulator in residency training: a validity and model analysis.

    Science.gov (United States)

    Colaco, Marc; Balica, Adrian; Su, Daniel; Barone, Joseph

    2013-03-01

    Robotic surgery is an important new tool in many surgical procedures, and training curriculums must adapt to this new technology. Robotic surgical simulators have been developed as a means of providing training without the inherent risks of actual surgery. The purpose of this study is to evaluate the construct validity of the RoSS surgical simulator by correlating simulator performance with amount of time in training and to create a performance model in which time in training is a parameter. A total of eight residents with varying amounts of training were given access to the RoSS surgical simulator and were evaluated on performance of a simulated surgical task. This data was then used to create Akaike information criteria to compare goodness of fit. Participants were also given a questionnaire as to their experience with the simulator and their feelings about the use of simulators in training. Training time and performance within the simulator were shown to have a linear relationship. Correlations were high, with R (2) values of 0.95, 0.94, and 0.86 for each of the three performance metrics. Likelihood ratios were similarly high at 4.25 × 10(9), 10,950, and 362. Participant opinion showed that residents feel that robotic training is an important part of their education and that the simulator is an effective supplement. The RoSS surgical simulator accurately corresponds to training level and is a valid evaluation tool of training experience. These findings are encouraging for the use of robotic simulators in surgical training. PMID:27000895

  8. $b\\to ss{\\bar d} $ decay in Randall-Sundrum models

    CERN Document Server

    Lü, Cai-Dian; Qin, Qin

    2016-01-01

    The extremely small branching ratio of $b\\to ss{\\bar d}$ decay in the Standard Model makes it a suitable channel to explore new physics through various extensions of the Standard Model. We study this $\\Delta S=2$ process in Randall-Sundrum models, including the custodially protected and the bulk-Higgs Randall-Sundrum models. Exploring the parameter space of these models constrained by $K^0-\\bar K^0$ mixing and $B_s^0-\\bar B_s^0$ mixing, it suggests a possible accessibility of this decay in future experiments.

  9. Työhyvinvointi Holiday Club Saimaan vastaanottovirkailijoiden työssä

    OpenAIRE

    Mälkiä, Suvi

    2016-01-01

    Opinnäytetyön tavoitteena oli selvittää Holiday Club Saimaan vastaanottovirkailijoiden ajatuksia työhyvinvoinnista ja siitä, onko työn kuormittavuudella ja kiireellisyydellä vaikutusta työhyvinvointiin. Tutkimuksessa keskityttiin siihen millaiset asiat kuormittavat työssä, millaisena työhyvinvointi koetaan Holiday Club Saimaalla, miten työn sesonkilaatuisuus koetaan ja millaista on hyvä esimiestyö. Holiday Club Saimaa on Lappeenrannan Rauhassa, Saimaan rannalla sijaitseva suuri kylpylähot...

  10. Helsingin matkailullisen brändi-identiteetin vahvistuminen Helsinki-oppaan työssä

    OpenAIRE

    Riehunkangas, Juha; Oikarinen, Satu

    2010-01-01

    Tämä opinnäytetyö käsittelee Helsingin tavoittelemaa matkailubrändiä ja brändi-identiteetin vahvistumista Helsinki-oppaan työssä. Helsingin matkailustrategian 2009–2012 mukaan Helsinki tavoittelee vahvaa matkailubrändiä. Tutkimuksen tavoitteena oli selvittää, ovatko Helsinki-oppaiden tärkeinä pitämät ja korostamat vetovoimatekijät linjassa strategiassa määriteltyjen brändi-elementtien kanssa. Toisena tavoitteena oli selvittää Helsinki-oppaiden kiinnostus lisäkoulutukseen ja koulutusaiheisiin....

  11. Hoitotyön arvot käytännössä

    OpenAIRE

    Mankinen, Hanna; Lohilahti, Jenna

    2009-01-01

    Kainuussa on hoitotyöntekijöille vuonna 2007 jaettu Hoitotyön arvokirja. Kirjaan Kainuun maakunta -kuntayhtymä on määritellyt viisi hoitotyön arvoa suuntaviivoiksi kainuulaiselle hoitotyölle. Näitä arvoja ovat ihmisarvon kunnioittaminen, huolenpito ja lähimmäisenrakkaus, terveys, oikeudenmukaisuus ja kehitysmyönteisyys. Käsittelemme opinnäytetyössämme sairaanhoitajien toteuttamaa potilasohjausta Hoitotyön arvokirjan näkökul-masta. Toimeksiantajana toimi Kainuun maakunta -kuntayhtymä. Opin...

  12. Microstructures of an Ultrafine Grained SS400 Steel in an Industrial Scale

    Institute of Scientific and Technical Information of China (English)

    2006-01-01

    The microstructures of a SS400 steel after thermomechanical control process (TMCP) in an industrial production were observed by optical microscope, scanning electron microscope (SEM) and transmission electron microscope (TEM). The results indicated that the size of ferrite grains was 4~5μm, and the volume fraction of ferrite was around 70%. The types of the ultrafine ferrite grains were analyzed and the strengthening mechanisms were discussed. The results show that the ultrafine ferrite grains came from three processes, i.e.deformation induced ferrite transformation (DIFT), dynamic recrystallization of ferrite and accelerated cooling process. The increase in the strength of the material was mainly due to the grain refining.

  13. Hoitohenkilökunnan kokemuksia rumpumusiikin vaikutuksesta muistisairaan potilaan kuntouttavassa hoitotyössä

    OpenAIRE

    Ilonen, Hanna

    2016-01-01

    Opinnäytetyön tarkoituksena oli selvittää muistisairaan potilaan kuntouttavaan hoitotyöhön osallistuvien hoitajien ja hoitohenkilökunnan kokemuksia rummun käytöstä musiikin välineenä hoitotyössä psykogeriatrisella osastolla Pirkanmaan sairaanhoitopiirissä. Opinnäytetyön tehtävinä oli selvittää hoitohenkilökunnan kokemuksia siitä, miten musiikin käyttö vaikuttaa psykogeriatrisessa osastohoidossa oleviin muistisairaisiin potilaisiin, minkälainen työväline musiikki on osana kuntouttavaa hoitotyö...

  14. Purification and preliminary characterization of a xylanase from Thermomyces lanuginosus strain SS-8

    OpenAIRE

    Shrivastava, Smriti; Shukla, Pratyoosh; Mukhopadhyay, Kunal

    2011-01-01

    Thermomyces lanuginosus SS-8 was isolated from soil samples that had been collected from near self-heating plant material and its extracellular cellulase-free xylanase purified approximately 160-fold using ion exchange chromatography and continuous elution electrophoresis. This xylanase was thermoactive (optimum temperature 60 °C) at pH 6.0 and had a molecular weight of 23.79 kDa as indicated by SDS-PAGE electrophoresis. The xylanase rapidly hydrolyzed xylan directly to xylose without the pro...

  15. Synthesis and antioxidant evaluation of (S,S)- and (R,R)-secoisolariciresinol diglucosides (SDGs)

    OpenAIRE

    Mishra, Om P; Simmons, Nicholas; Tyagi, Sonia; Pietrofesa, Ralph; Shuvaev, Vladimir V.; Valiulin, Roman A.; Heretsch, Philipp; Nicolaou, K.C.; Christofidou-Solomidou, Melpo

    2013-01-01

    Secoisolariciresinol diglucosides (SDGs) (S,S)-SDG-1 (major isomer in flaxseed) and (R,R)-SDG-2 (minor isomer in flaxseed) were synthesized from vanillin via secoisolariciresinol (6) and glucosyl donor 7 through a concise route that involved chromatographic separation of diastereomeric diglucoside derivatives (S,S)-8 and (R,R)-9. Synthetic (S,S)-SDG-1 and (R,R)-SDG-2 exhibited potent antioxidant properties (EC50 = 292.17 ± 27.71 μM and 331.94 ± 21.21 μM, respectively) which compared well with...

  16. Hengellisen väkivallan tunnistaminen sosiaali- ja terveysalan työssä

    OpenAIRE

    Kuokkanen, Elina

    2011-01-01

    Opinnäytetyöni aihe on hengellinen väkivalta ja sen tunnistaminen sosiaali- ja terveysalan työssä. Sosiaalialan työhön keskeisesti kuuluu väkivallan tunnistaminen, kohtaaminen ja ehkäiseminen. Hengellinen väkivalta erotetaan nykyisin usein omaksi väkivallan lajikseen, mutta siitä huolimatta se on aiheena melko tuntematon ja vähän tutkittu. Työni tavoitteena oli lisätä sosiaali- ja terveysalan ammattilaisten tietämystä aiheesta ja mahdollisuuksia aiheen käsittelyyn asiakastilanteis...

  17. UHV compatible Al to SS joining through electro magnetic forming technique

    Science.gov (United States)

    Prasad, N. K.; Ram, Y.; Sabharwal, T. P.; Pathak, K.; Kumar, M.; Matkar, A. W.; Rajawat, R. K.

    2008-05-01

    Fusion welding, which is the most versatile and widely used method of metallurgical bonding, can be troublesome in certain applications like dissimilar metal joining for Ultra High Vacuum requirements. Welding of Stainless Steel to Aluminum by conventional technique is very difficult due to thermal & metallurgical incompatibility. Consequently a number of non-conventional specialized joining techniques were developed. Joining of SS to Al by Electro Magnetic Forming (EMF) method is an effort in this direction. The paper discusses in detail the joining process, joint design and the results achieved.

  18. Myynnin haasteet kauneudenhoitoalan B2B-myyntityössä

    OpenAIRE

    Pulkkinen, Rosa

    2016-01-01

    Opinnäytetyön tavoitteena oli selvittää, mitä haasteita myyntiedustajat kohtaavat business- to-business-myyntityössään kauneudenhoitoalan organisaatiossa. Tutkimuksessa selvitettiin yhteistyöyrityksen myyntihenkilöstön työtyytyväisyys yleisellä tasolla. Tutkimuksen yhteistyökumppanina toimi suomalainen kauneudenhoitoalan yritys, joka myy ja markkinoi kauneus- ja hyvinvointituotteita alan ammattilaisille ja kuluttajille. Työn teoriaosuudessa käsiteltiin myyntiedustajan ammattia, työnkuvaa,...

  19. An evaluation of microbial growth and corrosion of 316L SS in glycol/seawater mixtures

    Science.gov (United States)

    Lee, Jason S.; Ray, Richard I.; Lowe, Kristine L.; Jones-Meehan, Joanne; Little, Brenda J.

    2003-01-01

    Glycol/seawater mixtures containing > 50% glycol inhibit corrosion of 316L stainless steel and do not support bacterial growth. The results indicate bacteria are able to use low concentrations of glycol (10%) as a growth medium, but bacterial growth decreased with increasing glycol concentration. Pitting potential, determined by anodic polarization, was used to evaluate susceptibility of 316L SS to corrosion in seawater-contaminated glycol. Mixture containing a minimum concentration of 50% propylene glycol-based coolant inhibited pitting corrosion. A slightly higher minimum concentration (55%) was needed for corrosion protection in ethylene glycol mixtures.

  20. Restonomiopiskelijoiden työssä käyminen ja sen vaikutus opintoihin

    OpenAIRE

    Virtanen, Sanna-Mari

    2011-01-01

    Opinnäytetyön aiheena oli restonomiopiskelijoiden työssä käyminen ja sen vaikutus opintoihin. Aihe on ajankohtainen, sillä etenkin matkailu- ja ravitsemisalalla työnteko opintojen ohella on vuosi vuodelta yleistynyt. Monella opiskelijalla ei ole vaihtoehtoja, vaan töitä on tehtävä, jos haluaa säilyttää edes jonkinlaisen elintason. Opinnäytetyön tuottamista uusista tiedoista hyötyivät Saimaan ammattikorkeakoulu ja sen opettajat ja opiskelijat. Työntekoa ja sen vaikutuksia opiskeluun tutkit...

  1. Asiakkaan osallistumisen ja suoriutumisen arviointi Hoitokoti Kuikanpesässä

    OpenAIRE

    Härkönen, Matti; Hirvonen, Kati

    2012-01-01

    Opinnäytetyön toimeksiantaja ja yhteistyökumppani oli Ilomantsissa toimiva Hoitokoti Kuikanpesä, joka tarjoaa palveluasumista sekä tehostettua palveluasumista mielenterveys- ja mielenterveyspäihdekuntoutujille. Hoitokoti on aloittanut toimintansa keväällä 2011. Opinnäytetyön tavoitteena oli löytää luotettava ja työelämässä psykiatristen asiakkaiden kanssa käyttökelpoiseksi havaittu toimintaterapian arviointikokonaisuus hoitokodin toimintatera- peutin käyttöön. Tarkoituksena oli, että opinn...

  2. Psykiatrisen sairaanhoitajan valmiudet ja kokemukset kuuron potilaan hoitotyössä

    OpenAIRE

    Siljanen, Kaisa

    2012-01-01

    Opinnäytetyön tutkimuksen tarkoituksena oli kuvata psykiatristen sairaanhoitajien valmiuksia kuuron potilaan kohtaamiseen. Tarkoituksena oli myös selvittää psykiatristen sairaanhoitajien kokemuksia kuuron potilaan hoitotyöstä. Opinnäytetyön tavoitteena oli saada lisää tietoa ja siten kehittää kuuron potilaan psykiatrista hoitotyötä. Sairaanhoitajat voivat hyödyntää tutkimuksen tuloksia kohdatessaan kuuron potilaan niin psykiatrisessa kuin somaattisessakin hoitotyössä. Kuurot ovat viittoma...

  3. Asiakaspalvelusta ylivoimatekijä esimiestyöllä : Case Osuuskauppa PeeÄssä

    OpenAIRE

    Makkonen, Reetta; Vallioniemi, Sarianne

    2013-01-01

    Asiakaspalvelu ja sen laatu ovat tänä päivänä yrityksen tärkeimpiä kilpailukeinoja. Jatkuvasti etsitään tapoja erottua kilpailijoista, ja siihen on alettu kiinnittämään erityisen paljon huomiota. Asiakaspalvelun laadun kehittäminen on jatkuva prosessi, johon vaaditaan tavoitteellista laatu- ja muutosjohtamista sekä henkilöstön sitoutumista. Tämän opinnäytetyön toimeksianto on tullut Osuuskauppa PeeÄssän johdolta ja tutkimuksen tarkoituksena on sel-vittää kuinka esimiestyöllä voidaan vaik...

  4. Multiresolution imaging of mantle reflectivity structure using SS and P'P' precursors

    Science.gov (United States)

    Schultz, Ryan; Gu, Yu J.

    2013-10-01

    Knowledge of the mantle reflectivity structure is highly dependent on our ability to efficiently extract, and properly interpret, small seismic arrivals. Among the various data types and techniques, long-period SS/PP precursors and high-frequency receiver functions are routinely utilized to increase the confidence of the recovered mantle stratifications at distinct spatial scales. However, low resolution and a complex Fresnel zone are glaring weaknesses of SS precursors, while over-reliance on receiver distribution is a formidable challenge for the analysis of converted waves from oceanic regions. A promising high frequency alternative to receiver functions is P'P' precursors, which are capable of resolving mantle structures at vertical and lateral resolution of ˜5 and ˜200 km, respectively, owing to their spectral content, shallow angle of incidence and near-symmetric Fresnel zones. This study presents a novel processing method for both SS (or PP) and P'P' precursors based on deconvolution, stacking, Radon transform and depth migration. A suite of synthetic tests is performed to quantify the fidelity and stability of this method under different data conditions. Our multiresolution survey of the mantle at targeted areas near Nazca-South America subduction zone reveal both olivine and garnet related transitions at depths below 400 km. We attribute a depressed 660 to thermal variations, whereas compositional variations atop the upper-mantle transition zone are needed to explain the diminished or highly complex reflected/scattered signals from the 410 km discontinuity. We also observe prominent P'P' reflections within the transition zone, and the anomalous amplitudes near the plate boundary zone indicate a sharp (˜10 km thick) transition that likely resonates with the frequency content of P'P' precursors. The migration of SS precursors in this study shows no evidence of split 660 reflections, but potential majorite-ilmenite (590-640 km) and ilmenite

  5. Elektronisen tanssimusiikin tuotanto : Tuotantotekniikka teoriassa ja käytännössä

    OpenAIRE

    Kaapro, Ville

    2010-01-01

    Opinnäytetyöni käsittelee elektronisen tanssimusiikin tuotantoa, työvaiheita ja tuotannossa käytettäviä työvälineitä. Käsittelen aihetta teoreettisesta näkökulmasta käyttäen apuna kirjallista lähdeaineistoa sekä omaa kokemusta ja taustatietoa aiheeseen liittyen. Opinnäytetyöni tavoitteena on antaa kokonaiskuva elektronisen tanssimusiikin tuottamisen työvaiheista ja tyypillisistä tuotantomenetelmistä esimerkkien kautta. Olen jakanut aihealueen työvaiheisiin, joita käsittelen selkeässä j...

  6. Valmentamisen mahdollisuudet työelämässä

    OpenAIRE

    Sarpo, Joni

    2012-01-01

    Tämän opinnäytetyön tarkoituksena oli tutkia, millaisia mahdollisuuksia valmentamisella on työelämässä. Päätavoitteena oli saada esille erilaisten ihmisten käsityksiä ja kokemuksia valmentamisesta ja sen hyödyistä. Aihe oli erittäin ajankohtainen, sillä uusi sukupolvi on astumassa työelämään ja sen odotukset työelämältä ja johtamiselta sisältävät samoja asioita kuin valmentaminen. Tutkimus toteutettiin laadullisena ja aineisto kerättiin teemahaastatteluilla. Haastattelut purettiin narrati...

  7. Mobiilitietoturvan kehittäminen Halti Oy:ssä ohjeiston avulla

    OpenAIRE

    Hyyryläinen, Olli

    2011-01-01

    Mobiililaitteista älypuhelimien ja sormitietokoneiden määrä on kasvanut räjähdysmäisesti viimeisen ‎viiden vuoden aikana. Perinteisten matkapuhelintenkin ominaisuudet ovat kehittyneet siihen ‎pisteeseen, että matkapuhelimia on jo vaikeaa luokitella niihin, jotka ovat tai eivät ole ‎älypuhelimia. Yrityksissä kehitys on huomattu, ja entistä useammalla työntekijällä on käytössään ‎älypuhelin laajakaistanopeuteen kykeneväksi mainostetulla langattomalla tiedonsiirtoyhteydellä. ‎Internet on työntek...

  8. Psykososiaalinen tuki ja monikulttuurinen ohjaus- ja neuvontatyö nuorten maahanmuuttajien opetusryhmässä

    OpenAIRE

    Paunila, Vaula

    2014-01-01

    TIIVISTELMÄ Paunila, Vaula. Psykososiaalinen tuki ja monikulttuurinen ohjaus- ja neuvontatyö nuorten maahanmuuttajien opetusryhmässä. Opinnäytetyö, syksy 2014, 138 s, 3 liitettä. Diakonia-ammattikorkeakoulu, Sosiaalialan koulutusohjelma, sosionomi (AMK). Kehittämispainotteisen opinnäytetyön lähtökohtana oli tehdä näkyväksi Espoossa kehitetty monialainen toimintamalli oppivelvollisuusiän ylittäneiden maahanmuuttajanuorten kotoutumisen edistämisessä. Opinnäytetyön tarkoituksena oli k...

  9. Hoitotyöntekijöiden kokemuksia palliatiivisen potilaan hoitotyössä

    OpenAIRE

    Haikola, Kati

    2008-01-01

    Tämän opinnäytetyön tarkoituksena on kuvata, miten hoitotyöntekijät kokevat palliatiivisen potilaan hoitotyön. Opinnäytetyössämme kuvataan myös hoitotyön tekijöiden jaksamista ja palliatiivisen potilaan hoitotyön laatua. Tutkimuksemme on kvalitatiivinen ja avoimella kyselylomakkeella tehty tutkimus. Aineisto kerättiin erään palliatiivisen osaston hoitohenkilökunnalta. Vastauksia saatiin kymmenen kappaletta. Saatu aineisto analysoitiin induktiivisella sisällönanalyysilla. Tutkimustuloste...

  10. Aggressiivisesti käyttäytyvän potilaan kohtaaminen hoitotyössä

    OpenAIRE

    Andersen, Asta

    2015-01-01

    Opinnäytetyö toteutettiin Oulun kaupunginsairaalan akuuttivuodeosastolla. Metodologisena lähestymistapana oli toimintatutkimus, jonka ensimmäisen syklin tarkoituksena oli kuvata hoitotyöntekijöiden (hoitajien) kokemuksia uhka- ja väkivaltatilanteista. Tutkimustehtäväksi muodostui, minkälaisia uhka- ja väkivaltatilanteita hoitajat ovat kokeneet hoitotyössä? Tavoitteena oli kehittää hoitajien valmiuksia kohdata aggressiivinen potilas yhteisten pelisääntöjen ja vuorovaikutusosaamisen avulla. Tut...

  11. Evidence for 4-chlorobenzoic acid dehalogenation mediated by plasmids related to pSS50. [Alcaligenes

    Energy Technology Data Exchange (ETDEWEB)

    Layton, A.C.; Sanseverino, J.; Wallace, W.; Corcoran, C.; Sayler, G.S. (Univ. of Tennessee, Knoxville (United States))

    1992-01-01

    The potential for polychlorinated biphenyl biodegradation is of environmental interest because of the toxicity and bioaccumulation of these compounds. Biodegradation studies on 4-chlorobiphenyl (4CB), a model for polychlorinated biphenyl degradation, have focused mainly on the genes and enzymes that permit the degradation of 4CB to 4-chlorobenzoate (4CBA). Most biphenyl- and polychlorinated biphenyl-degrading strains accumulate the corresponding chlorobenzoic acids without degrading the chlorobenzoate to CO{sub 2}. The study demonstrated that the 4-chlorobiphenyl-degrading Alcaligenes sp. strain ALP83 can degrade 4-chlorobenzoate to 4-hydroxybenzoate. The dehalogenase activity is correlated with a 10-kb fragment carried on plasmid pSS70.

  12. Comparison of the microbial communities in solid-state anaerobic digestion (SS-AD) reactors operated at mesophilic and thermophilic temperatures.

    Science.gov (United States)

    Li, Yueh-Fen; Nelson, Michael C; Chen, Po-Hsu; Graf, Joerg; Li, Yebo; Yu, Zhongtang

    2015-01-01

    The microbiomes involved in liquid anaerobic digestion process have been investigated extensively, but the microbiomes underpinning solid-state anaerobic digestion (SS-AD) are poorly understood. In this study, microbiome composition and temporal succession in batch SS-AD reactors, operated at mesophilic or thermophilic temperatures, were investigated using Illumina sequencing of 16S rRNA gene amplicons. A greater microbial richness and evenness were found in the mesophilic than in the thermophilic SS-AD reactors. Firmicutes accounted for 60 and 82 % of the total Bacteria in the mesophilic and in the thermophilic SS-AD reactors, respectively. The genus Methanothermobacter dominated the Archaea in the thermophilic SS-AD reactors, while Methanoculleus predominated in the mesophilic SS-AD reactors. Interestingly, the data suggest syntrophic acetate oxidation coupled with hydrogenotrophic methanogenesis as an important pathway for biogas production during the thermophilic SS-AD. Canonical correspondence analysis (CCA) showed that temperature was the most influential factor in shaping the microbiomes in the SS-AD reactors. Thermotogae showed strong positive correlation with operation temperature, while Fibrobacteres, Lentisphaerae, Spirochaetes, and Tenericutes were positively correlated with daily biogas yield. This study provided new insight into the microbiome that drives SS-AD process, and the findings may help advance understanding of the microbiome in SS-AD reactors and the design and operation of SS-AD systems.

  13. Effects of co-habitation between Anopheles gambiae s.s. and Culex quinquefasciatus aquatic stages on life history traits

    Directory of Open Access Journals (Sweden)

    Kweka Eliningaya J

    2012-02-01

    Full Text Available Abstract Background The effective measures for the control of malaria and filariasis vectors can be achieved by targeting immature stages of anopheline and culicine mosquitoes in productive habitat. To design this strategy, the mechanisms (like biotic interactions with conspecifc and heterospecific larvae regulating mosquito aquatic stages survivorship, development time and the size of emerging adults should be understood. This study explored the effect of co-habitation between An. gambiae s.s. and Cx. quinquefasciatus on different life history traits of both species under different densities and constant food supply in the habitats of the same size under semi-natural conditions. Methods Experiments were set up with three combinations; Cx. quinquefasciatus alone (single species treatment, An. gambiae s.s. alone (single species treatment; and An. gambiae s.s. with Cx. quiquefasciatus (co-habitation treatment in different densities in semi field situation. Results The effect of co-habitation of An. gambiae s.s. and Cx. quinquefasciatus was found to principally affect three parameters. The wing-lengths (a proxy measure of body size of An. gambiae s.s. in co-habitation treatments were significantly shorter in both females and males than in An. gambiae s.s single species treatments. In Cx. quinquefasciatus, no significant differences in wing-length were observed between the single species and co-habitation treatments. Daily survival rates were not significantly different between co-habitation and single species treatments for both An. gambiae s.s. and Cx. quinquefasciatus. Developmental time was found to be significantly different with single species treatments developing better than co-habitation treatments. Sex ratio was found to be significantly different from the proportion of 0.5 among single and co-habitation treatments species at different densities. Single species treatments had more males than females emerging while in co

  14. The Borrelia burgdorferi telomere resolvase, ResT, anneals ssDNA complexed with its cognate ssDNA-binding protein.

    Science.gov (United States)

    Huang, Shu Hui; Kobryn, Kerri

    2016-06-20

    Spirochetes of the genus Borrelia possess unusual genomes that consist in a linear chromosome and multiple linear and circular plasmids. The linear replicons are terminated by covalently closed hairpin ends, referred to as hairpin telomeres. The hairpin telomeres represent a simple solution to the end-replication problem. Deoxyribonucleic acid replication initiates internally and proceeds bidirectionally toward the hairpin telomeres. The telomere resolvase, ResT, forms the hairpin telomeres from replicated telomere intermediates in a reaction with similarities to those promoted by type IB topoisomerases and tyrosine recombinases. ResT has also been shown to possess DNA single-strand annealing activity. We report here that ResT promotes single-strand annealing of both free DNA strands and ssDNA complexed with single-stranded DNA binding protein (SSB). The annealing of complementary strands bound by SSB requires a ResT-SSB interaction that is mediated by the conserved amphipathic C-terminal tail of SSB. These properties of ResT are similar to those demonstrated for the recombination mediator protein, RecO, of the RecF pathway. Borrelia burgdorferi is unusual in lacking identifiable homologs of the RecFOR proteins. We propose that ResT may provide missing RecFOR functions. PMID:27131360

  15. X-ray variability of SS 433: Evidence for supercritical accretion

    Science.gov (United States)

    Atapin, K. E.; Fabrika, S. N.

    2016-08-01

    We study the X-ray variability of SS 433 based on data from the ASCA observatory and the MAXI and RXTE/ASM monitoring missions. Based on the ASCA data, we have constructed the power spectrum of SS 433 in the frequency range from 10-6 to 0.1 Hz, which confirms the presence of a flat portion in the spectrum at frequencies 3 × 10-5-10-3 Hz. The periodic variability (precession, nutation, eclipses) begins to dominate significantly over the stochastic variability at lower frequencies, which does not allow the stochastic variability to be studied reliably. The model in which the flat portion extends to 9.5 × 10-6 Hz, while a power-law rise with an index of 2.6 occurs below provides the best agreement with the observations. The nutational oscillations of the jets with a period of about three days suggests that the time for the passage of material through the disk is less than this value. At frequencies below 4 × 10-6 Hz, the shape of the power spectrum probably does not reflect the disk structure but is determined by external factors, for example, by a change in the amount of material supplied by the donor. The flat portion can arise from a rapid decrease in the viscous time in the supercritical or radiative disk zones. The flat spectrum is associated with the variability of the X-ray jets that are formed in the supercritical disk region.

  16. X-ray Variability of SS 433: Evidence for Supercritical Accretion

    CERN Document Server

    Atapin, Kirill

    2016-01-01

    We study the X-ray variability of SS433 based on data from the ASCA observatory and the MAXI and RXTE/ASM monitoring missions. Based on the ASCA data, we have constructed the power spectrum of SS433 in the frequency range from $10^{-6}$ to 0.1 Hz, which confirms the presence of a flat portion (flat-topped noise) in the spectrum at frequencies $3\\times 10^{-5}$ - $10^{-3}$ Hz. The periodic variability (precession, nutation, eclipses) begins to dominate significantly over the stochastic variability at lower frequencies, which does not allow the stochastic variability to be studied reliably. The best agreement with the observations is reached by the model with the flat portion extending to $9.5\\times10^{-6}$ Hz and a power-law spectrum with index of 2.6 below that frequency. The jet nutation with a period of about three days suggests that the time for the passage of material through the disk is less than this value. Therefore, at frequencies below $4\\times10^{-6}$ Hz, the power spectrum probably does not reflect...

  17. Complete genome sequence of Treponema pallidum ssp. pallidum strain SS14 determined with oligonucleotide arrays

    Directory of Open Access Journals (Sweden)

    Sodergren Erica

    2008-05-01

    Full Text Available Abstract Background Syphilis spirochete Treponema pallidum ssp. pallidum remains the enigmatic pathogen, since no virulence factors have been identified and the pathogenesis of the disease is poorly understood. Increasing rates of new syphilis cases per year have been observed recently. Results The genome of the SS14 strain was sequenced to high accuracy by an oligonucleotide array strategy requiring hybridization to only three arrays (Comparative Genome Sequencing, CGS. Gaps in the resulting sequence were filled with targeted dideoxy-terminators (DDT sequencing and the sequence was confirmed by whole genome fingerprinting (WGF. When compared to the Nichols strain, 327 single nucleotide substitutions (224 transitions, 103 transversions, 14 deletions, and 18 insertions were found. On the proteome level, the highest frequency of amino acid-altering substitution polymorphisms was in novel genes, while the lowest was in housekeeping genes, as expected by their evolutionary conservation. Evidence was also found for hypervariable regions and multiple regions showing intrastrain heterogeneity in the T. pallidum chromosome. Conclusion The observed genetic changes do not have influence on the ability of Treponema pallidum to cause syphilitic infection, since both SS14 and Nichols are virulent in rabbit. However, this is the first assessment of the degree of variation between the two syphilis pathogens and paves the way for phylogenetic studies of this fascinating organism.

  18. Evolution in Binary and Triple Stars, with an application to SS Lac

    CERN Document Server

    Eggleton, P P; Eggleton, Peter P.; Kiseleva-Eggleton, Ludmila

    2001-01-01

    We present equations governing the way in which both the orbit and the intrinsic spins of stars in a close binary should evolve subject to a number of perturbing forces, including the effect of a third body in a possibly inclined wider orbit. We illustrate the solutions in some binary-star and triple-star situations: tidal friction in a wide but eccentric orbit of a radio pulsar about a B star, the Darwin and eccentricity instabilities in a more massive but shorter-period massive X-ray binary, and the interaction of tidal friction with Kozai cycles in a triple such as Algol (beta-Per), at an early stage in that star's life when all 3 components were ZAMS stars. We also attempt to model in some detail the interesting triple system SS Lac, which stopped eclipsing in about 1950. We find that our model of SS Lac is quite constrained by the relatively good observational data of this system, and leads to a specific inclination (29 deg) of the outer orbit relative to the inner orbit at epoch zero (1912). Although th...

  19. Effect of residual strain on radiation induced segregation in SS 304

    Energy Technology Data Exchange (ETDEWEB)

    Ahmedabadi, P., E-mail: adit@barc.gov.i [Materials Science Division, Bhabha Atomic Research Centre, Mumbai 400085 (India); Kain, V. [Materials Science Division, Bhabha Atomic Research Centre, Mumbai 400085 (India); Arora, K. [PEC University of Technology, Chandigarh (India); Samajdar, I. [Indian Institute of Technology Bombay, Powai, Mumbai 400076 (India)

    2011-04-15

    Research highlights: {yields} Effect of strain RIS in proton-irradiated SS 304 studied using EPR followed by AFM. {yields} Residual strain within grains reduced Cr depletion at grain boundaries due to RIS. {yields} Attack during EPR of irradiated SS 304 observed on linear features within grains. {yields} Strain-regions within grains decorated by point defects and appeared as linear features. - Abstract: The effect of residual strain on radiation-induced segregation in proton-irradiated type 304 stainless steel was investigated using electrochemical potentiokinetic reactivation (EPR) test followed by atomic force microscopic examination. The specimens were irradiated to 0.43 and 0.86 dpa. Microstructural observation after EPR of irradiated specimens showed negligible attack on grain boundaries. Attacked linear features were noticed after EPR testing of irradiated specimens while such features were not observed for un-irradiated as-received or irradiated solution-annealed specimen. The presence of linear features after EPR testing of irradiated specimens was attributed to decoration of strain-regions within the matrix by point-defects generated due to irradiation.

  20. ssDNA Pairing Accuracy Increases When Abasic Sites Divide Nucleotides into Small Groups.

    Directory of Open Access Journals (Sweden)

    Alexandra Peacock-Villada

    Full Text Available Accurate sequence dependent pairing of single-stranded DNA (ssDNA molecules plays an important role in gene chips, DNA origami, and polymerase chain reactions. In many assays accurate pairing depends on mismatched sequences melting at lower temperatures than matched sequences; however, for sequences longer than ~10 nucleotides, single mismatches and correct matches have melting temperature differences of less than 3°C. We demonstrate that appropriately grouping of 35 bases in ssDNA using abasic sites increases the difference between the melting temperature of correct bases and the melting temperature of mismatched base pairings. Importantly, in the presence of appropriately spaced abasic sites mismatches near one end of a long dsDNA destabilize the annealing at the other end much more effectively than in systems without the abasic sites, suggesting that the dsDNA melts more uniformly in the presence of appropriately spaced abasic sites. In sum, the presence of appropriately spaced abasic sites allows temperature to more accurately discriminate correct base pairings from incorrect ones.

  1. Symmetry in the changing jets of SS433 and its true distance from us

    CERN Document Server

    Bowler, K B M

    2004-01-01

    We present the deepest yet radio image of the Galactic jet source, SS433, which reveals over two full precession cycles (> 2 x 163 days) of the jet axis. Systematic and identifiable deviations from the traditional kinematical model for the jets are found: variations in jet speed, lasting for as long as tens of days, are necessary to match the detailed structure of each jet. It is remarkable that these variations are equal and opposite, matching the two jets simultaneously. This explains certain features of the correlated redshift residuals found in fits to the kinematic model of SS433 reported in the literature. Asymmetries in the image caused by light travel time enabled us to measure the jet speeds of particular points to be within a range from 0.24c to 0.28c, consistent with, yet determined independently from, the speeds derived from the famous moving optical emission lines. Taken together with the angular periodicity of the zigzag/corkscrew structure projected on the plane of the sky (produced by the prec...

  2. Interacting Large-Scale Magnetic Fields and Ionised Gas in the W50/SS433 System

    CERN Document Server

    Farnes, J S; Purcell, C; Sun, X H; Haverkorn, M; Lenc, E; O'Sullivan, S P; Akahori, T

    2016-01-01

    The W50/SS433 system is an unusual Galactic outflow-driven object of debatable origin. We have used the Australia Telescope Compact Array (ATCA) to observe a new 198 pointing mosaic, covering $3^\\circ \\times 2^\\circ$, and present the highest-sensitivity full-Stokes data of W50 to date using wide-field, wide-band imaging over a 2 GHz bandwidth centred at 2.1 GHz. We also present a complementary H$\\alpha$ mosaic created using the Isaac Newton Telescope Photometric H$\\alpha$ Survey of the Northern Galactic Plane (IPHAS). The magnetic structure of W50 is found to be consistent with the prevailing hypothesis that the nebula is a reanimated shell-like supernova remnant (SNR), that has been re-energised by the jets from SS433. We observe strong depolarization effects that correlate with diffuse H$\\alpha$ emission, likely due to spatially-varying Faraday rotation measure (RM) fluctuations of $\\ge48$ to 61 rad m$^{-2}$ on scales $\\le4.5$ to 6 pc. We also report the discovery of numerous, faint, H$\\alpha$ filaments tha...

  3. 姬松茸60Co辐射诱变菌株J5与原菌株J1蛋白质营养价值比较%Comparative research on protein nutritional value of the 60Co-radiated mutant strain J5 ofAgaricus blazei and its primary strain J1

    Institute of Scientific and Technical Information of China (English)

    刘朋虎; 江枝和; 翁伯琦; 肖淑霞; 雷锦桂; 唐翔虬

    2011-01-01

    The protein nutrition of60 Co-radiated mutant strain J, of Agaricus blazei and its original strain J1 in different growing flushes were studied by non-biological evaluation. The results showed that essential amino acid index, biological value, nutritional index, score of ratio coefficient of amino acid, chemical score and amino acid score of fruit bodies of mutant strain J5 were 1.02%, 2.26%, 2.93%, 11.72%, 9.35% and 5.13% higher than that of strain J, respectively. Four, five, five and six protein indexes respectively in the first, second, third and fourth growing flushes of mutant strains J5 were higher than that of its original strain J1. These results indicated that the protein nutrition of mutant strain J5 was higher than that of strain J1.%采用非生物学评价法对姬松茸60Co辐射诱变菌株J5和其原菌株J1不同潮次菇蛋白质的营养价值进行评价.结果表明:J5子实体的必需氨基酸指数、生物价、营养指数、氨基酸比值系数分、化学评分和氨基酸评分分别比J1高1.02%、2.26%、2.93%、11.72%、9.35%和5.13%;以上6项蛋白质评价指标中,J5第1、第2、第3、第4潮菇分别有4、5、5、6项指标高于J1,表明J5子实体的蛋白质营养价值高于J1.

  4. 水煮和微波处理对巴西蘑菇营养品质及抗氧化能力的影响%Effect of boiling and microwaving treatments on nutritional characteristics and antioxidant activities of mushroom(Agaricus blazei Murril)

    Institute of Scientific and Technical Information of China (English)

    张锐; 孙丽平; 翟庆娇; 常佳驹; 庄永亮

    2012-01-01

    The effects of boiling and microwaving on nutritional compositions and antioxidant activities of fresh and frozen A.blazei Murril(AbM) were evaluated and compared.The results showed that the contents of crude protein,ash,total soluble protein,total soluble sugar,vitamin C,and total phenolics decreased by both cooking treatments,but no significant changes were observed in lipids.Boiling and microwaving significantly changed the compositions of amino acids and decreased the contents of glucose,galactose and mannose,and the effect of boiling was higher than that of microwaving.Furthermore,boiling and microwaving significantly decreased the DPPH· scavenging activity and reducing power of AbM.Analysis of the relations between the antioxidant activities and total phenolic concentrations testified that the cooking treatments not only decreased the total phenolic contents,but also changed the types and relative amounts of phenolic compounds of AbM.%研究了水煮和微波加热处理对新鲜巴西蘑菇营养成分及其抗氧化能力的影响。结果表明,水煮和微波加热处理不同程度上降低了总蛋白质、灰分、可溶性蛋白质、可溶性多糖、总酚和VC的含量,而对脂肪的含量没有显著性变化。另外,加热处理明显改变了巴西蘑菇氨基酸和单糖的组成,微波处理的损失率显著低于水煮处理。加热处理对巴西蘑菇的还原力和清除DPPH·能力的影响显示,水煮和微波处理不仅降低了巴西蘑菇总酚含量,而且改变了其总酚的组成,水煮处理对其抗氧化能力的破坏能力明显高于微波处理。研究可为消费者日常生活合理烹调巴西蘑菇提供科学依据。

  5. 姬松茸多糖对树突状细胞Dectin-1、Syk及CARD9基因表达和T淋巴细胞增殖的影响%Effect of Polysaccharide Isolated from Agaricus Blazei Murill on mRNA Expression Levels of Dectin-1, Syk and CARD9 from Dendritic Cells

    Institute of Scientific and Technical Information of China (English)

    董海影; 高志影; 荣华; 金铭; 王俊平; 牛英才; 刘吉成

    2014-01-01

    目的:探讨低相对分子质量姬松茸多糖(low molecular weight polysaccharide isolated from Agaricus blazei Murill,LMPAB)对树突状细胞C型植物血凝素1(Dectin-1),脾酪氨酸激酶(Sky)和胱天蛋白募集域蛋白9(CARD9)基因表达和T淋巴细胞增殖的影响.方法:体外培养ICR小鼠骨髓来源的树突状细胞;通过流式细胞术检测树突状细胞表面CD11c和CD86分子的表达;采用WST方法检测LMPAB增强树突状细胞对T淋巴细胞的增殖作用;应用实时定量PCR方法检测Dectin-1,Sky和CARD9 mRNA的表达水平.结果:树突状细胞表面CD11c表达98.6%,CD86表达96.97%;与正常组(4.84±0.21)比较,LMPAB +OVA组(6.59±0.19)可增强树突状细胞对T淋巴细胞的增殖作用(P<0.05);与正常组Dectin-1 (6.13±0.27),Sky(0.36 ±0.11)和CARD9(1.85±0.20)比较,LMPAB+OVA组Dectin-1(8.25±0.19),Sky(1.63±0.16)和CARD9(4.40±0.19)明显升高,统计学具有显著意义(P<0.05).结论:所培养的髓系细胞为树突状细胞;LMPAB可通过调控树突状细胞Dectin-1,Sky和CARD9 mRNA的表达增强树突状细胞的免疫效能.

  6. Redox-sensitive micelles assembled from amphiphilic mPEG-PCL-SS-DTX conjugates for the delivery of docetaxel.

    Science.gov (United States)

    Zhang, Huiyuan; Wang, Kaiming; Zhang, Pei; He, Wenxiu; Song, Aixin; Luan, Yuxia

    2016-06-01

    Docetaxel (DTX) can produce anti-tumor effects by inhibiting cell growth and inducing apoptosis. However, the poor solubility of DTX restricts its application and its clinical formulation has caused serious adverse reaction due to the use of Tween-80. In the present study, DTX was conjugated to an amphiphilic di-block polymer to solve these problems. Methoxy poly(ethylene glycol)-poly(ε-caprolactone) (mPEG-PCL) was selected as the polymer skeleton and a redox sensitive disulfide bond was used as the linker between DTX and mPEG-PCL. The synthesized mPEG-PCL-SS-DTX conjugates were characterized by (1)H-nuclear magnetic resonance ((1)H NMR) and Fourier transform infrared spectroscopy (FTIR). Interestingly, the mPEG-PCL-SS-DTX conjugates could self-assemble into micelles in aqueous solution. The critical micelle concentration (CMC) of mPEG-PCL-SS-DTX micelles was about 2.3mgL(-1) determined using pyrene molecule fluorescent probe method while the size of mPEG-PCL-SS-DTX micelles was determined to be ca. 17.6nm and 116.0nm with a bimodal distribution by dynamic light scattering (DLS). The in vitro release results indicated that the as-prepared micelles exhibited a sustained release profile with good redox sensitive properties. In particular, the hemolytic toxicity test indicated the as-prepared mPEG-PCL-SS-DTX micelles had negligible hemolytic activity, demonstrating their safety in drug delivery system. Cytotoxicity assay of the mPEG-PCL-SS-DTX micelles verified their highly enhanced cytotoxicity to MCF-7/A and A549 cells. These results thus demonstrated that the present redox-sensitive mPEG-PCL-SS-DTX micelle was an efficient and safe sustained drug delivery system in the biomedical area.

  7. Introgression of Brown Norway CYP4A genes on to the Dahl salt-sensitive background restores vascular function in SS-5(BN) consomic rats.

    Science.gov (United States)

    Lukaszewicz, Kathleen M; Falck, John R; Manthati, Vijaya L; Lombard, Julian H

    2013-03-01

    The present study tested the hypothesis that the Dahl SS (salt-sensitive) rat has vascular dysfunction due, in part, to the up-regulation of the CYP4A/20-HETE (cytochrome P450 ω-hydroxylase 4A)/20-hydroxyeicosatetraenoic acid) system. To assess the role of vascular 20-HETE, SS rats were compared with SS-5(BN) consomic rats, carrying CYP4A alleles on chromosome 5 from the normotensive BN (Brown Norway) introgressed on to the SS genetic background. Cerebral arteries from SS-5(BN) rats had less CYP4A protein than arteries from SS rats fed either NS (normal-salt, 0.4% NaCl) or HS (high-salt, 4.0% NaCl) diet. ACh (acetylcholine)-induced dilation of MCAs (middle cerebral arteries) from SS and SS-5(BN) rats was present in SS-5(BN) rats fed on either an NS or HS diet, but absent in SS rats. In SS rats fed on either diet, ACh-induced dilation was restored by acute treatment with the CYP4A inhibitor DDMS (N-methyl-sulfonyl-12,12-dibromododec-11-enamide) or the 20-HETE antagonist 20-HEDE [20-hydroxyeicosa-6(Z),15(Z)-dienoic acid]. The restored response to ACh in DDMS-treated SS rats was inhibited by L-NAME (N(G)nitro-L-arginine methyl ester) and unaffected by indomethacin or MS-PPOH [N-methylsulfonyl-6-(2-propargyloxyphenyl)hexanamide]. Vascular relaxation responses to the NO donor C(5)FeN(6)Na(2)O were intact in both SS and SS-5(BN) rats and unaffected by the acute addition of DDMS, indicating that the vascular dysfunction of the SS rat is due to a reduced bioavailability of NO instead of failure of the VSMCs (vascular smooth muscle cells) to respond to the vasodilator. Superoxide levels in cerebral arteries of SS-5(BN) rats [evaluated semi-quantitatively by DHE (dihydroethidium) fluorescence] were lower than those in the arteries of SS rats. These findings indicate that SS rats have an up-regulation of the CYP4A/20-HETE pathway resulting in elevated ROS (reactive oxygen species) and reduced NO bioavailability causing vascular dysfunction.

  8. SS18 together with animal-specific factors defines human BAF-type SWI/SNF complexes.

    Directory of Open Access Journals (Sweden)

    Evelien Middeljans

    Full Text Available BACKGROUND: Nucleosome translocation along DNA is catalyzed by eukaryotic SNF2-type ATPases. One class of SNF2-ATPases is distinguished by the presence of a C-terminal bromodomain and is conserved from yeast to man and plants. This class of SNF2 enzymes forms rather large protein complexes that are collectively called SWI/SNF complexes. They are involved in transcription and DNA repair. Two broad types of SWI/SNF complexes have been reported in the literature; PBAF and BAF. These are distinguished by the inclusion or not of polybromo and several ARID subunits. Here we investigated human SS18, a protein that is conserved in plants and animals. SS18 is a putative SWI/SNF subunit which has been implicated in the etiology of synovial sarcomas by virtue of being a target for oncogenic chromosomal translocations that underlie synovial sarcomas. METHODOLOGY/PRINCIPAL FINDINGS: We pursued a proteomic approach whereby the SS18 open reading frame was fused to a tandem affinity purification tag and expressed in amenable human cells. The fusion permitted efficient and exclusive purification of so-called BAF-type SWI/SNF complexes which bear ARID1A/BAF250a or ARID1B/BAF250b subunits. This demonstrates that SS18 is a BAF subtype-specific SWI/SNF complex subunit. The same result was obtained when using the SS18-SSX1 oncogenic translocation product. Furthermore, SS18L1, DPF1, DPF2, DPF3, BRD9, BCL7A, BCL7B and BCL7C were identified. 'Complex walking' showed that they all co-purify with each other, defining human BAF-type complexes. By contrast,we demonstrate that human PHF10 is part of the PBAF complex, which harbors both ARID2/BAF200 and polybromo/BAF180 subunits, but not SS18 and nor the above BAF-specific subunits. CONCLUSIONS/SIGNIFICANCE: SWI/SNF complexes are found in most eukaryotes and in the course of evolution new SWI/SNF subunits appeared. SS18 is found in plants as well as animals. Our results suggest that in both protostome and deuterostome animals

  9. Melanoma inhibitor of apoptosis protein (ML-IAP) specific cytotoxic T lymphocytes cross-react with an epitope from the auto-antigen SS56

    DEFF Research Database (Denmark)

    Baek Sørensen, Rikke; Faurschou, Mikkel; Troelsen, Lone;

    2009-01-01

    A large proportion of melanoma patients host a spontaneous T-cell response specifically against ML-IAP-derived peptides. In this study, we describe that some ML-IAP-specific cytotoxic T cells isolated from melanoma patients cross react with an epitope from the auto-antigen SS56. SS56 is a recently...... described target of autoantibody responses in Sjögren's syndrome (SS) as well as systemic lupus erythematosus (SLE). Here, we describe that SS56 is also an auto-antigen for T cells in SS and SLE. Hence, SS56-specific T cells could readily be detected in circulation and among the infiltrating cells of SLE...... skin lesions. SS56-specific T cells were able to lyse target cells presenting the peptide epitope on the surface. Notably, SS56-specific CD8 T cells isolated from an SS patient cross reacted with the ML-IAP epitope. This early evidence of a target for auto-reactive CTL in SS and SLE patients...

  10. Corrosion-erosion test of SS316L grain boundary engineering material (GBEM) in lead bismuth flowing loop

    Energy Technology Data Exchange (ETDEWEB)

    Saito, Shigeru, E-mail: saito.shigeru@jaea.go.jp [JAEA, Tokai-mura, Ibaraki-ken 319-1195 (Japan); Kikuchi, Kenji [Ibaraki Univ., Frontier Research Center, Tokai-mura, Ibaraki-ken 319-1106 (Japan); Hamaguchi, Dai [JAEA, Tokai-mura, Ibaraki-ken 319-1195 (Japan); Tezuka, Masao [Sukegawa Electic Co., Takahagi-shi, Ibaraki-ken 318-0004 (Japan); Miyagi, Masanori; Kokawa, Hiroyuki [Tohoku Univ., Sendai-shi, Miyagi-ken 980-8579 (Japan); Watanabe, Seiichi [Hokkaido Univ., Sapporo-shi, Hokkai-do 060-8628 (Japan)

    2012-12-15

    To evaluate the lifetime of structural materials utilized in a spallation neutron source, corrosion tests in lead-bismuth eutectic (LBE) have been done at JAEA. Austenitic steels are preferable as the structural material for ADS. However, previous studies have revealed that austenitic steel SS316 shows severe corrosion-erosion in LBE because of LBE penetration through grain boundaries and separation of grains. So it was considered that GBE (grain-boundary engineered) materials may be effective to improve the corrosion resistance of austenitic steels in LBE. In this study, the results of corrosion tests on austenitic steel SS316L-BM (base metal) and SS316L-GBEM (grain-boundary-engineered material) under flowing LBE conditions will be reported. The corrosion test was performed using the JAEA lead-bismuth material corrosion loop (JLBL-1). The experimental conditions were as follows: The high and low temperature parts of the loop were 450 Degree-Sign C and 350 Degree-Sign C, respectively. The flow velocity at the test specimens was about 0.7 m/s. The oxygen concentration in LBE was not controlled and was estimated to have been very low. After the 3600 h of operation, macroscopic, SEM, and SIM observations and EDX analysis were carried out. The results showed that the corrosion depth and LBE penetration through the grain boundaries of the 316SS-GBEM were smaller than those of the 316SS-BM.

  11. Corrosion-erosion test of SS316L grain boundary engineering material (GBEM) in lead bismuth flowing loop

    Science.gov (United States)

    Saito, Shigeru; Kikuchi, Kenji; Hamaguchi, Dai; Tezuka, Masao; Miyagi, Masanori; Kokawa, Hiroyuki; Watanabe, Seiichi

    2012-12-01

    To evaluate the lifetime of structural materials utilized in a spallation neutron source, corrosion tests in lead-bismuth eutectic (LBE) have been done at JAEA. Austenitic steels are preferable as the structural material for ADS. However, previous studies have revealed that austenitic steel SS316 shows severe corrosion-erosion in LBE because of LBE penetration through grain boundaries and separation of grains. So it was considered that GBE (grain-boundary engineered) materials may be effective to improve the corrosion resistance of austenitic steels in LBE. In this study, the results of corrosion tests on austenitic steel SS316L-BM (base metal) and SS316L-GBEM (grain-boundary-engineered material) under flowing LBE conditions will be reported. The corrosion test was performed using the JAEA lead-bismuth material corrosion loop (JLBL-1). The experimental conditions were as follows: The high and low temperature parts of the loop were 450 °C and 350 °C, respectively. The flow velocity at the test specimens was about 0.7 m/s. The oxygen concentration in LBE was not controlled and was estimated to have been very low. After the 3600 h of operation, macroscopic, SEM, and SIM observations and EDX analysis were carried out. The results showed that the corrosion depth and LBE penetration through the grain boundaries of the 316SS-GBEM were smaller than those of the 316SS-BM.

  12. The eSS rat, a nonobese model of disordered glucose and lipid metabolism and fatty liver

    Directory of Open Access Journals (Sweden)

    Daniele Stella M

    2010-03-01

    Full Text Available Abstract Background eSS is a rat model of type 2 diabetes characterized by fasting hyperglycemia, glucose intolerance, hyperinsulinemia and early hypertriglyceridemia. Diabetic symptoms worsen during the second year of life as insulin release decreases. In 12-month-old males a diffuse hepatic steatosis was detected. We report the disturbances of lipid metabolism of the model with regard to the diabetic syndrome. Methods The study was conducted in eight 12-month-old eSS male rats and seven age/weight matched eumetabolic Wistar rats fed with a complete commercial diet al libitum. Fasting plasmatic glucose, insulin, triglycerides, total cholesterol, low-density and high-density lipoprotein, and nonesterified fatty acids levels were measured. Very low density and intermediate-density lipoproteins were analyzed and hepatic lipase activity was determined. Results eSS rats developed hyperglycemia and hyperinsulinemia, indicating insulin resistance. Compared with controls, diabetic rats exhibited high plasmatic levels of NEFA, triglycerides (TG, total cholesterol (Chol and LDL-Chol while high-density lipoprotein (HDL cholesterol values were reduced. eSS rats also displayed TG-rich VLDL and IDL particles without changes in hepatic lipase activity. Conclusion The nonobese eSS rats develop a syndrome characterized by glucose and lipid disorders and hepatic steatosis that may provide new opportunities for studying the pathogenesis of human type 2 diabetes.

  13. 人KiSS-1基因克隆及其慢病毒载体的构建%Constructing lentivirus vector carrying human KiSS-1 gene cloning

    Institute of Scientific and Technical Information of China (English)

    于波; 张俐

    2008-01-01

    背景:慢性病毒载体技术是目前转基因中最有效和最成功的方法,技术操作简便.目的:克隆转移抑制基因KiSS-1基因不含信号肽的表达序列,构建人KiSS-1基因的慢病毒表达载体.设计、时间及地点:开放性实验于2006-09/2007-12在福建师范大学发育学院实验室完成.材料:载体pNL-IRES2-EGFP由福建师范大学发育学院实验室保存.方法:从人正常胎盘组织中提取总RNA,经反转录一聚合酶链反应得到KiSS-1基因开放阅读框cDNA序列,并将其克隆到慢病毒载体pNL-IRES2-EGFP中,构建其表达质粒pNL-IRES2-EGFP-KiSS-1.主要观察指标:KISS-1目的基因片段的克隆,重组表达质粒pNL-IRES2-EGFP-KiSS-1的酶切鉴定及测序.结果:经酶切鉴定和基因序列测定,证实重组入载体pNL-IRES2-EGFP的片段为目的基因开放阅读框的核苷酸序列.结论:成功构建了重组质粒pNL-IRES2-EGFP-KiSS-1.

  14. On-shell Diagrams, Gra{\\ss}mannians and Integrability for Form Factors

    CERN Document Server

    Frassek, Rouven; Nandan, Dhritiman; Wilhelm, Matthias

    2015-01-01

    We apply on-shell and integrability methods that have been developed in the context of scattering amplitudes in N=4 SYM theory to tree-level form factors of this theory. Focussing on the colour-ordered super form factors of the chiral part of the stress-energy multiplet as an example, we show how to systematically construct on-shell diagrams for these form factors with the minimal form factor as an additional building block. Moreover, we obtain analytic representations in terms of Gra{\\ss}mannian integrals in spinor helicity, twistor and momentum twistor variables. While Yangian invariance is broken by the operator insertion, we find that form factors are eigenstates of the integrable transfer matrix. As a consequence, we can construct them via the method of R operators, which also allows to introduce deformations that preserve the integrable structure.

  15. Luomu- ja lähiruoka-aamiaisten suunnittelu, toteutus ja seuranta yritys X:ssä

    OpenAIRE

    Kasurinen, Antero

    2010-01-01

    Opinnäytetyö toteutettiin toimeksiantona yritys X:ään. Yritys X halusi vastata asiakkaiden tarpeisiin ‎kehittämällä jo olemassa olevien aamiaisten rinnalle sekä luomu- että lähiruokaaamiaisen.lisäksi ‎opinnäytetyön tavoitteena oli tarkastella luomu- ja lähiruoka-aamiaisten myyntiä touko-joulukuun 2009 ‎ajalta. Opinnäytetyön pääongelmaksi muodostui, miten suunnitellaan, toteutetaan ja seurataan luomu- ja ‎lähiruoka-aamiaisia yritys X:ssä. Opinnäytetyön teoriaosassa käsitellään puolestaan luomu...

  16. X-Ray Lines from Hot Flows around White Dwarfs. Application to SS Cygni

    CERN Document Server

    Menou, K; Raymond, J; Menou, Kristen; Perna, Rosalba; Raymond, John

    2001-01-01

    Instead of possessing a boundary layer at the interface between a disk and their stellar surface, White Dwarfs (WDs) in quiescent Dwarf Novae (DN) could accrete via an Advection-Dominated Accretion Flow (ADAF), responsible for the X-ray Bremsstrahlung emission observed. Such a hot accretion flow is also expected to produce characteristic thermal line emission. Using SS Cyg as a specific example, we show that knowing a priori the inclination and the WD mass in quiescent DN makes X-ray line diagnostics powerful probes of the flow structure in these systems. Current X-ray instruments can discriminate, from their width, between lines emitted from a flow with a Keplerian rotation rate and those emitted at a substantially sub-Keplerian rate. This could be used to observationally test the property of energy advection, which is at the origin of the partial radial pressure support by the hot gas and the sub-Keplerian rotation rate in an ADAF.

  17. Uniaxial Time-Dependent Ratcheting of SS304 Stainless Steel at High Temperatures

    Institute of Scientific and Technical Information of China (English)

    KANG Guo-zheng; ZHANG Juan; SUN Ya-fang; KAN Qian-hua

    2007-01-01

    The uniaxial time-dependent strain cyclic behaviors and ratcheting of SS304 stainless steel were studied at high temperatures (350 ℃ and 700 ℃). The effects of straining and stressing rates, holding time at the peak and/or valley of each cycle in addition to ambient temperature on the cyclic softening/hardening behavior and ratcheting of the material were discussed. It can be seen from experimental results that the material presents remarkable time dependence at 700 ℃, and the ratcheting strain depends greatly on the stressing rate, holding time and ambient temperature. Some significant conclusions are obtained, which are useful to build a constitutive model describing the time-dependent cyclic deformation of the material.

  18. Purification and preliminary characterization of a xylanase from Thermomyces lanuginosus strain SS-8.

    Science.gov (United States)

    Shrivastava, Smriti; Shukla, Pratyoosh; Mukhopadhyay, Kunal

    2011-12-01

    Thermomyces lanuginosus SS-8 was isolated from soil samples that had been collected from near self-heating plant material and its extracellular cellulase-free xylanase purified approximately 160-fold using ion exchange chromatography and continuous elution electrophoresis. This xylanase was thermoactive (optimum temperature 60 °C) at pH 6.0 and had a molecular weight of 23.79 kDa as indicated by SDS-PAGE electrophoresis. The xylanase rapidly hydrolyzed xylan directly to xylose without the production of intermediary xylo-oligosaccharides within 15 min of incubation under optimum conditions. This trait of rapidly degrading xylan to xylose as a sole end-product could have biotechnological potential in degradation of agro-wastes for bioethanol manufacturing industry. PMID:22558544

  19. Kansanedustajien avustajien työmotivaatio eduskuntaryhmässä

    OpenAIRE

    Kiuttu, Mikko

    2014-01-01

    Tämän opinnäytetyön aiheena on kansanedustajien avustajien työmotivaatio eduskunta-ryhmässä. Tutkimuksen tarkoituksena on selvittää, mitkä tekijät motivoivat erään edus-kuntaryhmän kansanedustajien avustajia. Työn tavoitteena on myös etsiä ratkaisuja, joilla työmotivaatiota voidaan lisätä. Opinnäytetyön tulokset auttavat osaltaan vielä paremman esimiestyön ja työyhteisön toimivuuden luomisessa. Opinnäytetyön teoreettisena viitekehyksenä on työmotivaation eri osa-alueita. Maslow´n tarvehi...

  20. Myyntihenkilöstön rekrytoinnin kehittäminen Yritys Oy:ssä

    OpenAIRE

    Vatka-Horttanainen, Satu

    2011-01-01

    Tämän opinnäytetyön tarkoituksena oli kehittää Yritys Oy:n myyntihenkilöstön rekrytointia. Opinnäytetyössä luotiin kehitysehdotuksia myös myyntihenkilöstön henkilöstövaihtuvuuden pienentämiseksi. Tavoitteena oli löytää hyviä käytäntöjä ja menetelmiä myyntihenkilöstön hankintaan ja yrityksen houkuttelevuuteen. Tutkimuksen teoreettinen tietoperusta ja viitekehys rakennettiin henkilöstövoimavarojen strategista johtamista sekä rekrytointia koskevasta liiketalouden kirjallisuudesta. Se rakentu...

  1. An SS-TDMA system using onboard regenerative repeaters and baseband switch

    Science.gov (United States)

    Kato, S.; Samejima, S.; Yamamoto, H.

    A system configuration of a Satellite Switched Time Division Multiple Access (SS-TDMA) satellite communication system employing onboard regenerative repeaters, a baseband switch and IF switch is proposed and discussed. The system proposed in this paper utilizes 30/20 GHz frequency bands and is composed of Trunk Transmission Systems and Satellite Digital Communication Systems which provide wide band digital communication links between customers directly. Interface conditions between terrestrial digital terminals and TDMA traffic terminals, and a TDMA frame structure for Trunk Transmission Systems are proposed and discussed. Moreover, a transmission power control scheme for multibeam satellite communication systems is proposed. Furthermore, the experimental results for a prototype onboard burst modem are presented showing good bit error ratio and weight performance.

  2. 5S:n käyttöönotto Nomet Oy:ssä

    OpenAIRE

    Nummi, Janne

    2012-01-01

    Tämän opinnäytetyön aiheena on 5S-toimintamallin käyttöönotto Nomet Oy:n tuotannossa, Tampereen Vehmaisissa. Nomet on alihankintakonepaja, joka on erikoistunut lastuavaan työstöön. Yrityksessä on päätetty ottaa käyttöön Lean- valmistusfilosofiaan pohjautuvaa tuotannon uudistamista. 5S on yksi Leanin monista työkaluista, josta on hyvä lähteä alkuun. 5S:n päätavoitteena on parantaa tuotantoprosesseja siisteyden, järjestyksen ja standardisoinnin avulla. Työssä on esitelty kahdesta tuotannon ...

  3. Evaluation of liquid metal embrittlement of SS304 by Cd and Cd-Al solutions

    Energy Technology Data Exchange (ETDEWEB)

    Thomas, J.K.; Iyer, N.C. (Westinghouse Savannah River Co., Aiken, SC (United States)); Begley, J.A. (Westinghouse Science and Technology Center, Pittsburgh, PA (United States))

    1992-01-01

    The susceptibility of stainless steel 304 to liquid metal embrittlement (LME) by cadmium (Cd) and cadmium-aluminum (Cd-Al) solutions was examined as part of a failure evaluation for SS304-clad cadmium reactor safety rods which had been exposed to elevated temperatures. The active, or cadmium (Cd) bearing, portion of the safety rod consists of a 0.756 in. diameter aluminum allow (Al-6061) core, a 0.05 in. thick Cd layer, and a 0.042 in. thick Type 304 stainless steel cladding. The safety rod thermal tests were conducted as part of a program to define the response of reactor core components to a hypothetical LOCA for the Savannah River Site (SRS) production reactor. LME was considered as a potential failure mechanism based on the nature of the failure and susceptibility of austenitic stainless steels to embrittlement by other liquid metals.

  4. Evaluation of liquid metal embrittlement of SS304 by Cd and Cd-Al solutions

    Energy Technology Data Exchange (ETDEWEB)

    Thomas, J.K.; Iyer, N.C. [Westinghouse Savannah River Co., Aiken, SC (United States); Begley, J.A. [Westinghouse Science and Technology Center, Pittsburgh, PA (United States)

    1992-07-01

    The susceptibility of stainless steel 304 to liquid metal embrittlement (LME) by cadmium (Cd) and cadmium-aluminum (Cd-Al) solutions was examined as part of a failure evaluation for SS304-clad cadmium reactor safety rods which had been exposed to elevated temperatures. The active, or cadmium (Cd) bearing, portion of the safety rod consists of a 0.756 in. diameter aluminum allow (Al-6061) core, a 0.05 in. thick Cd layer, and a 0.042 in. thick Type 304 stainless steel cladding. The safety rod thermal tests were conducted as part of a program to define the response of reactor core components to a hypothetical LOCA for the Savannah River Site (SRS) production reactor. LME was considered as a potential failure mechanism based on the nature of the failure and susceptibility of austenitic stainless steels to embrittlement by other liquid metals.

  5. Jet propulsion of wind ejecta from a major flare in the black hole microquasar SS433

    CERN Document Server

    Blundell, Katherine

    2011-01-01

    We present direct evidence, from Adaptive-Optics near-infra-red imaging, of the jets in the Galactic microquasar SS433 interacting with enhanced wind-outflow off the accretion disc that surrounds the black hole in this system. Radiant quantities of gas are transported significant distances away from the black hole approximately perpendicular to the accretion disc from which the wind emanates. We suggest that the material that comprised the resulting "bow-tie" structure is associated with a major flare that the system exhibited ten months prior to the observations. During this flare, excess matter was expelled by the accretion disc as an enhanced wind, which in turn is "snow-ploughed", or propelled, out by the much faster jets that move at approximately a quarter of the speed of light. Successive instances of such bow-ties may be responsible for the large-scale X-ray cones observed across the W50 nebula by ROSAT.

  6. PREFACE: The 4th Symposium on the Mechanics of Slender Structures (MoSS2013)

    Science.gov (United States)

    Cao, Dengqing; Kaczmarczyk, Stefan

    2013-07-01

    This volume of Journal of Physics: Conference Series contains papers presented at the 4th Symposium on the Mechanics of Slender Structures (MoSS2013) run under the auspices of the Institute of Physics Applied Mechanics Group and hosted by Harbin Institute of Technology (China) from 7-9 January 2013. The conference has been organized in collaboration with the Technical Committee on Vibration and Sound of the American Society of Mechanical Engineers and follows a one day seminar on Ropes, Cables, Belts and Chains: Theory and Applications and the MoSS2006 symposium held at the University of Northampton (UK) in 2004 and 2006, respectively, the MoSS2008 symposium held at the University of Maryland Baltimore County (USA) in 2008 and the MoSS2010 symposium hosted by Mondragon University and held in San Sebastian (Spain) in 2010. The remit of the Symposium on the Mechanics of Slender Structures series involves a broad range of scientific areas. Applications of slender structures include terrestrial, marine and space systems. Moving elastic elements such as ropes, cables, belts and tethers are pivotal components of many engineering systems. Their lengths often vary when the system is in operation. The applications include vertical transportation installations and, more recently, space tether propulsion systems. Traction drive elevator installations employ ropes and belts of variable length as a means of suspension, and also for the compensation of tensile forces over the traction sheave. In cranes and mine hoists, cables and ropes are subject to length variation in order to carry payloads. Tethers experiencing extension and retraction are important components of offshore and marine installations, as well as being proposed for a variety of different space vehicle propulsion systems based on different applications of momentum exchange and electrodynamic interactions with planetary magnetic fields. Furthermore, cables and slender rods are used extensively in civil engineering

  7. Isännöitsijänä asunto-osakeyhtiössä

    OpenAIRE

    Alajärvi, Annika

    2015-01-01

    Tämän opinnäytetyön aiheena oli isännöitsijänä asunto-osakeyhtiössä. Tutkimustehtävänä oli tarkastella isännöitsijän ominaisuuksia, ammattitaidon ylläpitämistä, tehtäväkenttää sekä hyvän isännöitsijän piirteitä. Tarkastelun kohteena olivat asunto-osakeyhtiöiden isännöitsijät ja tutkimus perustui isännöitsijöiden näkemyksiin tutkimusaiheesta. Tutkimuskysymykset olivat: minkälaisia henkilöitä toimii isännöitsijöinä, miten isännöitsijä ylläpitää ammattitaitoaan, millainen on isännöitsijän tehtäv...

  8. DotA传奇人物回顾:ToSsGirL

    Institute of Scientific and Technical Information of China (English)

    2010-01-01

    首先声明,此ToSsGirL不是彼TossGirl,他没有前凸后翘的身材也没有令韩国众宅男YY不已的脸蛋,他只是一个深爱DotA最后却不得不放弃DotA的,为梦想不断前行的DotA玩家。或许现在的DotA爱好者们并不熟悉这个人,甚至都从来没听说过这个ID,但这并不妨碍他在中国DotA的历史中写下浓墨重彩的一笔,也并不妨碍他开启中国电子竞技的新篇章。

  9. Yksityisyyden suoja työelämässä

    OpenAIRE

    Korttinen, Kaisa

    2011-01-01

    Tämän opinnäytetyön aiheena on yksityisyyden suoja työelämässä. Opinnäyte-työ sisältää periaatteet henkilötietojen käsittelystä työelämän eri vaiheissa, ku-ten työnhaun ja työsuhteen aikana. Opinnäytetyön tarkoituksena on kerätä hyvä tietopaketti työelämän henkilötietojen käsittelyyn liittyvistä laeista ja niihin liitty-vistä perusperiaatteista. Opinnäytetyön teoriaosuus perustuu lainsäädäntöön, joka sisältää työnantajan velvollisuudet rekisterinpitäjänä ja työntekijöiden oikeudet rekiste...

  10. Sovellettu väriteoria 3D-ympäristössä

    OpenAIRE

    Enges, Iiro

    2012-01-01

    Tämä opinnäyte on kirjoittajan henkilökohtainen kehittämisprojekti, jossa pyritään tutkimaan käytännön väriteoriaa kuvakerronnassa ja soveltamaan sitä digitaalisen 3D-ympäristön työskentelyyn. Työn tavoitteena on tutkia käytännönläheisesti, millaisia värejä tehokkaan kuvan tekoon käytetään ja miten ne voidaan tietoisten valintojen kautta saavuttaa 3D-ympäristössä. Tekstiosuudessa keskitytään värin ja siihen liittyvien teorioiden selittämiseen, käsitellen värin fysikaalisia, visuaalisia ja...

  11. Reweldability test of irradiated SS316 by the TIG welding method

    Science.gov (United States)

    Tsuchiya, Kunihiko; Kawamura, Hiroshi; Oyamada, Rokuro

    1996-10-01

    Stainless steel is a candidate material for the structural material in fusion reactors. Rewelding of irradiated materials will have a large impact on the design and the maintenance of in-vessel components. In the present work, the welding specimens made of type 316 stainless steel were irradiated in JMTR (Japan materials testing reactor) to a fast neutron fluence of ˜2.0 × 10 20 n/cm 2 ( E > 1 MeV) at a temperature of ˜200°C. The rewelding of unirradiated and/or irradiated stainless steel was performed by the tungsten inert gas (TIG) welding method and the weldments of unirradiated and/or irradiated SS316 were characterized by tensile testing (test temp.: 20°C and 200°C), hardness, metallographical observation and SEM/XMA analyses.

  12. Migration and release behavior of tritium in SS316 at ambient temperature

    Science.gov (United States)

    Torikai, Y.; Murata, D.; Penzhorn, R.-D.; Akaishi, K.; Watanabe, K.; Matsuyama, M.

    2007-06-01

    BIXS measurements indicate that immersion into water or chemical etching of SS316 contaminated with tritium at moderate temperatures causes an immediate reduction of the outermost surface concentration of tritium. The fraction of surface tritium removed by water, i.e. 30-50%, is small in comparison to the total tritium present in the specimen. Allowing a specimen to age whose surface and subsurface had been removed by etching up to a depth where the concentration of tritium is mostly constant revealed that within a few months a re-growth of tritium up to a saturation value higher than half of that originally present on the specimen takes place. Concurrently, a small but steady liberation of tritium at rates increasing from 0.1 to 0.3 kBq/h was noticed.

  13. Effect of microstructure on radiation induced segregation and depletion in ion irradiated SS316 steel

    Energy Technology Data Exchange (ETDEWEB)

    Jin, Hyung Ha; Kwon, Sang Chul; Kwon, Jun Hyun [Korea Atomic Energy Research Institute, Daejeon (Korea, Republic of)

    2011-10-15

    Irradiation assisted stress corrosion cracking (IASCC), void swelling and irradiation induced hardening are caused by change of characteristics of material by neutron irradiation, stress state of material and environmental situation. It has been known that chemical compositions varies at grain boundary (GB) significantly with fluence level and the depletion of Cr element at GB has been considered as one of important factors causing material degradation, especially, IASCC in austenitic stainless steel. However, experimental results of IASCC under PWR condition were directly not connected with Cr depletion phenomenon by neutron irradiation. Because the mechanism of IASCC under PWR has not yet been clearly understood in spite of many energetic researches, fundamental researches about radiation induced segregation and depletion in irradiated austenitic stainless steels have been attracted again. In this work, an effect of residual microstructure on radiation induced segregation and depletion of alloy elements at GB was investigated in ion irradiated SS316 steel using transmission electron microscope (TEM) with energy dispersive spectrometer (EDS)

  14. Influence of agitation speeds and aeration rates on the Xylanase activity of Aspergillus niger SS7

    Directory of Open Access Journals (Sweden)

    Yasser Bakri

    2011-08-01

    Full Text Available In this study, the effect of agitation and aeration rates on xylanase activity of Aspergillus niger SS7 in 3-litre stirred tank bioreactor was investigated. The agitation rates tested were 100, 200 and 300 rpm at each airflow rates of 0.5, 1.0 and 1.5 vvm. The maximum xylanase activity in mono- agitator system was at the agitation speed of 200 rpm and aeration rate of 1.0 vvm. In bi-agitator system, at low agitation speed (100 rpm, the xylanase activity was enhanced by 13% compared to mono- agitator system for an aeration rate of 1.0 vvm. Xylanase productivity in continuous culture was higher by approximately 3.5 times than in batch culture.

  15. Uudistuva johtajuus ja Z-sukupolvi työelämässä

    OpenAIRE

    Aakkula, Eveliina

    2012-01-01

    Opinnäytetyössä käsitellään epäjohtamista, joka on uusi johtamisen käsite, jonka ovat luoneet Janne Tienari ja Rebekka Piekkari kirjassaan Z ja epäjohtaminen. Terminä epäjohtaminen ei ole virallinen, eikä tule välttämättä vakiintumaan johtajuuden käsitteiden joukkoon. Tämä uusi johtamisen malli liitetään vahvasti Z-sukupolven tulemiseen työmarkkinoille. Z-sukupolvea ovat 1990-luvulla syntyneet. Johtajuus on tärkeä aihe, jonka käsittelemiseen löytyy aina uusia näkökulmia, joiden toimivuutta vo...

  16. PCS-kuvien käyttöönotto kahdessa päiväkotiryhmässä

    OpenAIRE

    Kallioinen, Annika

    2015-01-01

    Opinnäytetyöni aiheena oli PCS -kuvien käyttöönotto kahdessa Porin päiväkotiryhmässä. PCS- kuvat (Picture Communication Symbols) ovat usein käytössä integroiduissa päiväkotiryhmissä, mutta tavallisissa ryhmissä niiden käyttö on vielä vähäistä. Kuitenkin lähes jokaisessa ryhmässä on nykyään erityistä tukea tarvitsevia lapsia tai maahanmuuttajalapsia, jotka tarvitsevat kuvia tuomaan apua ja tukea kommunikoinnissaan. Opinnäytetyöni tarkoitus oli auttaa kahden eri päiväkodin tavallista ryhmää ott...

  17. Nykyaikaisten mobiililaitteiden tietoturva yrityskäytössä: case: Etelä-Savon Tietohallinto Oy

    OpenAIRE

    Kinnaslampi, Emppu

    2012-01-01

    Tämän opinnäytetyön tarkoituksena oli tutkia, minkälaisia tietoturvauhkia nykyaikaisiin mobiililaitteisiin, kuten älypuhelimiin ja tabletteihin kohdistuu erityisesti yrityskäytössä, ja miten näitä uhkia voidaan torjua ja ennaltaehkäistä. Työssä pyrin selvittämään, miten sekä organisaatio että käyttäjä itse voivat mobiililaitteidensa tietoturvaa aktiivisesti omilla toimillaan parantaa sekä minkälaiset valmiudet nykyään merkittävimmissä mobiilikäyttöjärjestelmissä Androidissa, iOS:ssä, Windows ...

  18. Development, Methodology and Potential of the New Universal Visual Scoring System (UniViSS for Caries Detection and Diagnosis

    Directory of Open Access Journals (Sweden)

    Thomas Oehme

    2009-09-01

    Full Text Available Given the limitations of adjunct caries detection and diagnostic tools, e.g., imperfect validity and reproducibility, as well as the difficulties in controlling all possible confounding factors, the need for an objective visual caries detection and diagnosis system has become evident. Our work has therefore aimed at systematizing caries lesions with the Universal Visual Scoring System (UniViSS for occlusal and smooth surface lesions, which can be used for primary and permanent teeth, as well as under clinical, epidemiological, public health and laboratory conditions. Besides the description of the development and methodology of UniViSS, it is shown that UniViSS allows an accurate and reproducible classification of caries lesions on occlusal surfaces.

  19. 葡萄糖可调控pSS-SPCAD-proinsulin载体的构建及鉴定

    Institute of Scientific and Technical Information of China (English)

    刘艳霞; 胡平; 栗夏连

    2009-01-01

    目的 构建一葡萄糖可调控pSS-SPCAD-proinsulin表达载体,模仿胰岛β细胞生理功能.方法 从PhIns-ABC扩增出SS和proinsulin,与pEGFP-N1,一人工合成多核苷酸(SPCAD),通过酶切、连接、转化,构建出pSS-SPCAD-proinsulin载体.结果 经PCR,测序证实,pSS-SPCAD-proinsulin栽体构建成功.结论 该表达载体的构建为在胰岛素的分泌水平上实现葡萄糖对胰岛素的调控提供了材料.

  20. MicNeSs: genotyping microsatellite loci from a collection of (NGS) reads.

    Science.gov (United States)

    Suez, Marie; Behdenna, Abdelkader; Brouillet, Sophie; Graça, Paula; Higuet, Dominique; Achaz, Guillaume

    2016-03-01

    Microsatellites are widely used in population genetics to uncover recent evolutionary events. They are typically genotyped using capillary sequencer, which capacity is usually limited to 9, at most 12 loci for each run, and which analysis is a tedious task that is performed by hand. With the rise of next-generation sequencing (NGS), a much larger number of loci and individuals are available from sequencing: for example, on a single run of a GS Junior, 28 loci from 96 individuals are sequenced with a 30X cover. We have developed an algorithm to automatically and efficiently genotype microsatellites from a collection of reads sorted by individual (e.g. specific PCR amplifications of a locus or a collection of reads that encompass a locus of interest). As the sequencing and the PCR amplification introduce artefactual insertions or deletions, the set of reads from a single microsatellite allele shows several length variants. The algorithm infers, without alignment, the true unknown allele(s) of each individual from the observed distributions of microsatellites length of all individuals. MicNeSs, a python implementation of the algorithm, can be used to genotype any microsatellite locus from any organism and has been tested on 454 pyrosequencing data of several loci from fruit flies (a model species) and red deers (a nonmodel species). Without any parallelization, it automatically genotypes 22 loci from 441 individuals in 11 hours on a standard computer. The comparison of MicNeSs inferences to the standard method shows an excellent agreement, with some differences illustrating the pros and cons of both methods.

  1. La música religiosa a la ciutat de Barcelona (ss. XVII-XIX

    Directory of Open Access Journals (Sweden)

    Codina i Giol, Daniel

    2002-12-01

    Full Text Available A chronological account of the Kapellmeisters of the three principal sites in Barcelona: the Cathedral, the church of Santa Maria del Mar and the Palau de la Comtessa, including their compositions -most of which are ignored today as the scores have been lost- based on the printed booklets of the popular chants and oratories retrieved from the XVII to the XIX centuries. The account in question draws, in part, on the printed Catàleg dels villancets i oratoris impresos de la Biblioteca de Montserrat (ss. XVII-XIX, which the very author of this study has himself prepared and which is to be published in due course. The said catalogue will undoubtedly make a valuable contribution to the study of the history of music.

    [ca] Relació històrica i ordenada dels mestres de capella deis tres centres principals de la ciutat de Barcelona: la Catedral, Santa Maria del Mar i el Palau de la Comtessa i llurs obres -la majoria desconegudes perquè se n'ha perdut la música escrita- a partir deis Ilibrets impresos deis villancets i deis oratoris, en el període dels segles XVII-XIX. El treball aprofita part de l'obra Catàleg dels villancets i oratoris impresos de la Biblioteca de Montserrat (ss. XVII-XIX, que el mateix autor de l'article ha preparat i que està en curs d'edició i és una mostra de la utilitat d'aquest Catàleg per a conèixer de manera mes precisa la historia musical.

  2. Effect of renewed SS-cream on spinal somatosensory evoked potential in rabbits

    Institute of Scientific and Technical Information of China (English)

    LongTian; Zhong-ChengXin; HuaXin; JieFu; Yi-MingYuan; Wu-JiangLiu; ChunYang

    2004-01-01

    Aim:The effect of a renewed SS-cream (RSSC) on the treatment of premature ejaculation (PE) was evaluated and compared with the original SS-cream (OSSC).Methods: Sixty male white New Zealand rabbits,weighing 2.5kg-3.0kg,were divided at random into 3 groups:the RSSC,OSSC and placebo groups.The spinal somatosensory evoked potential (SSEP) elicited by electric stimulation of the glans penis with disk electrode was investigated with an electrophysiograph (Poseidomn,Shanghai,China) before and 10,30 and 60 min after drug or placebo application on the glans.The Onset and the N1 latencies and the amplitude of SSEP were recorded and analyzed. Results:There was no significant difference (P>0.05) in the mean Onset and N1 latency of SSEP among the 3 groups before drug application.Compared with the pre-application value,the mean Onset and N 1 latencies in the RSSC and OSSC groups were significantly prolonged at 10,30 and 60 min after treatment (P0.05) in the placebo group.The mean Onset latency of RSSC at 10 and 30 min and that of OSSC at 30 min were significantly delayed (P<0.05) compared with the placebo group.The mean N1 latency of RSSC at 30 and 60 min and that of OSSC group at 30 min were also significantly delayed (P<0.05).Conclusion:RSSC delays the latencies of SSEP,suggesting a local desensitizing effect on the sensory receptor of the glans penis dorsal nerve,which provides the potential for PE treatment.The desensitizing effect of RSSC is higher than that of OSSC.( Asian J Androl 2004 Mar;6:15-18)

  3. Oxide induced corrosion on the welded stainless steels SS 2352 and 2353

    International Nuclear Information System (INIS)

    The pitting corrosion properties have been investigated in welded and unwelded condition by polarization tests in sodium chloride solutions. The two steels were TIG welded without adding welding material and as shielding on the bottom side argon gas containing 2, 26 or 99 ppm oxygen was used. In some tests low breakthrough potentials were received, without discovering any pitting corrosion in the specimen surfaces. The unwelded SS 2352 steel had a critical (lowest) pitting temperature (CPT) of 5 degrees C in the more concentrated solution. For the same steel with weld pitting corrosion was obtained at 5 degrees C, which was the lowest temperature for the tests. Thus the CPT value was lower than 5 degrees C, but by looking at the pitting corrosion potentials the following conclusion could be drawn: Welding with higher oxygen content in the shielding gas implied lower pitting corrosion resistance. For the SS 2353 steel the CPT values were 25 and 27.5 degrees C for material without weld, in contact with the more concentrated and the more dilute solution respectively. Welded material was all through more sensitive to pitting corrosion, and the CPT values were 15-17.5, 15 and 5-10 degrees C for welded areas which had been gas shielded with argon containing 2, 26 and 99 ppm oxygen respectively. The result thus showed that welding with shielding gas containing maximum about 30 ppm oxygen does not substantially affect the pitting corrosion properties. Post treatment of the welding areas increased the pitting corrosion resistance. Acid pickling implied the highest pitting corrosion resistance with 15 degrees C as CPT value for the 2353 steel in the more concentrated solution. Steel brushing implied an obvious increase to the pitting corrosion resistance compared to untreated weld areas and the same statement could be done for sand blasted surfaces. (10 refs., 16 tabs., 11 figs.)

  4. Microstructural, thermo-optical, mechanical and tribological behaviours of vacuum heat treated ultra thin SS304 foils

    Science.gov (United States)

    Palai, Debajyoti; Bhattacharya, Manjima; Basu Mallick, Amitava; Bera, Parthasarathi; Sharma, Anand Kumar; Mukhopadhyay, Anoop Kumar; Dey, Arjun

    2016-09-01

    The purpose of this present study was to evaluate the effect of vacuum (i.e., 10-5 mbar) heat treatment at 300 to 1100 °C on morphological, thermo-optical, mechanical and tribological properties of 75 μm thin SS304 foils. Microstructural, morphological and surface properties of the foils were characterized by field emission scanning electron microscopy (FESEM), profilometry, x-ray photoelectron spectroscopy (XPS) and water contact angle (WCA) measurement techniques, respectively. The x-ray diffraction (XRD) was employed to identify the phase of SS304 foils after different heat treatment temperatures. Thermo-optical properties such as solar absorptance (α s), reflectance (ρ s) and IR emittance (ɛ ir) were also evaluated. The nanomechanical properties i.e., nanohardness (H) and Young’s modulus (E) of as-received and heat treated SS304 foils were evaluated by the nanoindentation technique. Tribological and tensile properties i.e., yield strength (σ y) and percentage of elongation of different heat treated foils were also investigated by single pass scratch tests and universal testing machine, respectively. Noteworthy grain growth of SS304 was observed beyond vacuum heat treatment temperature of 700 °C. As a consequence, the magnitudes of both H and E data of SS304 were significantly decreased beyond the vacuum heat treatment temperature of 700 °C. Further, the hardness data followed the well known Hall-Petch relationship. On the other hand, the tribological property of SS304 was significantly deteriorated beyond the vacuum heat treatment temperature of 700 °C. The tensile strength of the foils was also altered after the heat treatment.

  5. Study of diffusion bonding of WL10 to SS joining with and without titanium interlayer using thermo mechanical simulator

    International Nuclear Information System (INIS)

    The sound joining of Tungsten alloy (W+1%LaO3) material with Stainless Steel material (SS316L) is demanded for He-cooled divertor for demo like Tokamak in near future application. The life of the divertor finger can be assessed by quality of the joining between two the materials. Due to significantly large difference of thermal mismatch, the joining of WL10 and SS material is appeared to be difficult one. The Helium cooled modular divertor is expected to face with the heat load of 10MW/m2 with operation temperature at W tile ∼ 1700°C and 700°C for WL10 to SS region (thimble). There are possible ways for joining of these two materials such as vacuum brazing route, diffusion bonding route etc. In this paper, diffusion bonding route was followed to prepare the joining of the WL10 material and SS material using Thermo mechanical simulator machine (Gleeble 3800). Diffusion bonding of WL10 and SS material has been carried out at various temperatures 800 °C, 850°C, 900 °C, 950 °C and 1000 °C with uniaxial pressure 5 MPa to 20 MPa with the hold time of 15 Min and 30 Min with and without titanium interlayer. The joint specimens have been investigated by NDT (UT by immersion probe), microstructural examination and hardness measurement. In this paper, the methodology for the preparation of diffusion bonding and the optimization of the diffusion bonding parameters for the joining of WL10 and SS material will be discussed. (author)

  6. Investigating the presence of predatory bacteria on algal bloom samples using a T6SS gene marker.

    Science.gov (United States)

    Hendricks, J.; Sison-Mangus, M.; Mehic, S.; McMahon, E.

    2015-12-01

    Predation is considered to be a major driving force in evolution and ecology, which has been observed affecting individual organisms, communities, and entire ecosystems. The type VI secretion system (T6SS) is an intermembranal protein complex identified in certain bacteria, which appears to have evolved strictly as a mechanism of predation. The effects of bacteria on phytoplankton physiology are still understudied, however, studies have shown that the interactions between bacteria that inhabit the phycosphere of phytoplankton can possibly result in coevolution of native host and microbiota. It is unclear if bacteria can prey upon other bacteria to gain advantages during periods of high phytoplankton density. Here, we investigate the predatory interactions between bacteria and analyze environmental samples for the presence of predatory bacterial genes in an effort to understand bacteria-bacteria and phytoplankton interactions during algal blooms. DNA were extracted from bacterial samples collected weekly from size-fractionated samples using 3.0 um and 0.2 um membrane filters at the Santa Cruz wharf. PCR amplification and gel visualization for the presence of T6SS gene was carried out on bloom and non-bloom samples. Moreover, we carried out a lab- based experiment to observe bacteria-bacteria interaction that may hint for the presence of predatory behavior between bacterial taxa. We observed what appeared to be a predatory biofilm formation between certain bacterial species. These bacteria, however, did not contain the T6SS genes. On the contrary the T6SS gene was discovered in some of the bloom samples gathered from the Santa Cruz wharf. It is still unclear if the predatory mechanisms facilitate the abundance of certain groups of bacteria that contain the T6SS genes during algal blooms, but our evidence suggest that bacterial predation through T6SS mechanism is present during bloom events.

  7. Effect of nitrogen on high temperature mechanical properties of type 316LN SS for fast reactor applications

    International Nuclear Information System (INIS)

    Long term creep, low cycle fatigue and creep-fatigue interaction properties as well as compatibility with liquid sodium coolant, govern the choice of materials for out-of-core structural components of sodium cooled fast reactors (SFRs). 316 L(N) SS containing 0.07 Wt.% nitrogen is the current preferred material for all the high temperature structural components of SFRs. For the prototype Fast Breeder Reactor also, which is nearing commissioning at Kalpakkam, 316 LN (SS) has been used for all the major high temperature structural components. It is proposed to design future SFRs for a life of at least 60 years. Therefore materials with higher creep and low cycle fatigue strength are required. Increasing the nitrogen content in 316L(N) SS is one of the approaches being pursued at Indira Gandhi Centre for Atomic Research (IGCAR) to develop a SFR structural material suitable for 60 years design life. Towards this, a strong R and D programme is currently underway at IGCAR. The effect of nitrogen in the range of 0.07 to 0.22 Wt.% on the tensile creep and low cycle fatigue behavior of 316 LN SS has been extensively investigated. Both tensile and creep strength were found to increase with increase in nitrogen content. High temperature low cycle fatigue life was found to peak in 316LN SS containing 0.14 Wt.%. The paper discusses the monotonic and cyclic deformation and fracture mechanisms by which nitrogen improves the creep and low cycle fatigue properties of 316 LN SS. (author)

  8. Biodegradable mixed MPEG-SS-2SA/TPGS micelles for triggered intracellular release of paclitaxel and reversing multidrug resistance

    Science.gov (United States)

    Dong, Kai; Yan, Yan; Wang, Pengchong; Shi, Xianpeng; Zhang, Lu; Wang, Ke; Xing, Jianfeng; Dong, Yalin

    2016-01-01

    In this study, a type of multifunctional mixed micelles were prepared by a novel biodegradable amphiphilic polymer (MPEG-SS-2SA) and a multidrug resistance (MDR) reversal agent (d-α-tocopheryl polyethylene glycol succinate, TPGS). The mixed micelles could achieve rapid intracellular drug release and reversal of MDR. First, the amphiphilic polymer, MPEG-SS-2SA, was synthesized through disulfide bonds between poly (ethylene glycol) monomethyl ether (MPEG) and stearic acid (SA). The structure of the obtained polymer was similar to poly (ethylene glycol)-phosphatidylethanolamine (PEG-PE). Then the mixed micelles, MPEG-SS-2SA/TPGS, were prepared by MPEG-SS-2SA and TPGS through the thin film hydration method and loaded paclitaxel (PTX) as the model drug. The in vitro release study revealed that the mixed micelles could rapidly release PTX within 24 h under a reductive environment because of the breaking of disulfide bonds. In cell experiments, the mixed micelles significantly inhibited the activity of mitochondrial respiratory complex II, also reduced the mitochondrial membrane potential, and the content of adenosine triphosphate, thus effectively inhibiting the efflux of PTX from cells. Moreover, in the confocal laser scanning microscopy, cellular uptake and 3-(4,5-dimethyl-thiazol-2-yl)-2,5-diphenyl-tetrazolium bromide assays, the MPEG-SS-2SA/TPGS micelles achieved faster release and more uptake of PTX in Michigan Cancer Foundation-7/PTX cells and showed better antitumor effects as compared with the insensitive control. In conclusion, the biodegradable mixed micelles, MPEG-SS-2SA/TPGS, could be potential vehicles for delivering hydrophobic chemotherapeutic drugs in MDR cancer therapy. PMID:27785018

  9. Rock-ravintolatoiminta : elävää rock-musiikkia ravintolaympäristössä

    OpenAIRE

    Sipilä, Esa; Väyliö, Jari

    2006-01-01

    Työn tavoitteena oli tutkia rock-ravintolatoimintaa ja elävää rock-musiikkia ravintolaympäristössä ravintolan, artistin ja asiakkaan näkökulmasta. Tutkimuksessa pyrittiin selvittämään rock-ravintolayrittämisen toimintatapoja ja kartoittamaan alan tämän hetkistä tilaa. Työssä tutustuttiin syvemmin suomalaiseen rock-ravintolatoimintaan yhtenä ravintolaelinkeinon haarana. Tutkimuksen aineisto kerättiin lomakehaastattelujen, teemahaastattelujen, osallistuvan havainnoinnin sekä kyselyjen avulla. T...

  10. Facebook-yhteisöpalvelun hyödyntäminen yrityksen markkinointiviestinnässä

    OpenAIRE

    Pajamäki, Ida

    2014-01-01

    Tämän opinnäytetyön tavoitteena oli selvittää, miksi yritysten olisi kannattavaa olla Facebook-yhteisöpalvelussa ja millaisia keinoja niillä on käytössään Facebook-palvelussa markkinoidessaan. Opinnäytetyössä rakennettiin työn toimeksiantajalle, Mainostoimisto Gurulle, oma Facebook-sivu. Guru on Turun ammattikorkeakoulun sisäinen mainostoimisto, jossa suunnitellaan kaikenlaista markkinointiviestintää opiskelijavoimin. Teoriaosassa käsiteltiin lyhyesti sosiaalista mediaa ja Facebook-palvel...

  11. Kirjallisuuskatsaus sairaanhoitajan voimaantumista edistävistä tekijöistä gerontologisessa hoitotyössä

    OpenAIRE

    Ahonala, Johanna

    2012-01-01

    Opinnäytetyön tarkoituksena oli selvittää, mitä gerontologinen hoitotyö on kirjallisuuden mukaan, sekä millaisia ovat sairaanhoitajien voimaantumista edistävät tekijät gerontologisessa hoitotyössä. Aineisto koostui systemaattisen kirjallisuuskatsauksen menetelmällä hankituista tutkimuksista sekä tutkimusartikkeleista (n=26). Tämän kirjallisuuskatsauksen tulosten mukaan gerontologisessa hoitotyössä korostuvat eettiset periaatteet, ihmisarvon kunnioittaminen sekä inhimillinen hoito. Vallan...

  12. Digitaalisen medianäytön hyödyntäminen markkinointiviestinnässä : case: Fazer

    OpenAIRE

    Lauanne, Marja

    2012-01-01

    Digitaalisuus on tuonut reaaliaikaisen viestinnän osaksi jokapäiväistä elämäämme. Tässä opinnäytetyössä tutkitaan yrityksen markkinointiviestintää ja digitaalisuuden viestintään tuomia muutoksia. Asiaa tarkastellaan markkinointiviestinnän suunnitteluprosessin kautta. Tutkitaan, kuinka markkinointiviestintäsuunnitelma rakennetaan, mitkä tekijät vaikuttava valintoihin prosessin sisällä ja kuinka saavutettuja tuloksia mitataan. Työn edetessä syvennytään tutkimaan digitaalista medianäyttöä ja s...

  13. Corrosion Inhibition by Tithonia diversifolia (Hemsl) A. Gray leaves extract for 304 SS in hydrochloric acid solution

    Science.gov (United States)

    Firdausi, S.; Kurniawan, F.

    2016-04-01

    The inhibition effect of Tithonia diversifolia (Hemsl) A. Gray leaves extract on the corrosion phenomena of 304 SS in 1 M HCl has been studied by polarization potentiodynamic. The powder of T. diversifolia was extracted by demineralized water which was cultivated in East Java, Indonesia. The extract was characterized by FTIR spectrophotometer. The presence of T. diversifolia can inhibit the corrosion rate of 304 SS. The efficiency inhibition value of 2 g/L T. diversifolia leaves extract reached up to 77.27% at room temperature.

  14. Mediator proteins orchestrate enzyme-ssDNA assembly during T4 recombination-dependent DNA replication and repair

    OpenAIRE

    Bleuit, Jill S.; Xu, Hang; Ma, Yujie; Wang, Tongsheng; Liu, Jie; Morrical, Scott W.

    2001-01-01

    Studies of recombination-dependent replication (RDR) in the T4 system have revealed the critical roles played by mediator proteins in the timely and productive loading of specific enzymes onto single-stranded DNA (ssDNA) during phage RDR processes. The T4 recombination mediator protein, uvsY, is necessary for the proper assembly of the T4 presynaptic filament (uvsX recombinase cooperatively bound to ssDNA), leading to the recombination-primed initiation of leading strand DNA synthesis. In the...

  15. Sosiaalipedagogisen hevostoiminnan tunnettavuus ja käyttö sosiaalisen kuntoutuksen keinona Jyväskylässä

    OpenAIRE

    Puustinen, Mari

    2015-01-01

    Hippolis- hevosalan osaamiskeskus ry:n päätehtävänä on toimia valtakunnallisena hevosalan yhteistyöorganisaationa. Hippolis osallistuu erilaisiin hevosalan yhteis-työprojekteihin, joiden aiheita ovat mm. hevosten käyttö hyvinvointipalveluissa ja hevonen kuntouttajana. Opinnäytetyön tavoitteena oli kartoittaa sosiaalipedagogisen hevostoiminnan tun-nettavuutta ja käyttöä Jyväskylässä. Sosiaalipedagoginen hevostoiminta on talliym-päristössä toteutettava sosiaalisen kuntoutuksen muoto. Se voi...

  16. Toimintaterapia maahanmuuttajatyössä : toimintaterapeuttien näkemyksiä työn haasteista ja mahdollisuuksista

    OpenAIRE

    Lappi, Salla

    2015-01-01

    Opinnäytetyössä tarkasteltiin, mitä toimintaterapia on maahanmuuttajatyössä ja minkälaisia haasteita tai mahdollisuuksia siihen suomalaisten toimintaterapeuttien näkemysten perusteella liittyy. Opinnäytetyön yhteistyökumppanina oli Tampereen kaupungin psykiatrian poliklinikka maahanmuuttajille. Yhteistyökumppanin lisäksi työn tuloksia voivat hyödyntää maahanmuuttajien parissa työskentelevät toimintaterapeutit, toimintaterapian opiskelijat ja opettajat sekä kaikki maahanmuuttajatyöstä ja sen k...

  17. Kulttuurierojen vaikutus yrityksen ja sidosryhmien välisessä yhteistyössä patentointialan yrityksessä

    OpenAIRE

    Saaristo, Anne

    2012-01-01

    Kulttuurieroja voidaan luokitella esimerkiksi alueen, uskonnon tai sukupolven mukaan. Maiden välisiä kulttuurieroja voidaan myös vertailla ja havainnollistaa kulttuuriulottuvuuksien avulla. Kulttuurien välisten erojen ymmärtäminen ja niiden huomioon ottaminen toiminnassa ja viestinnässä ovat oleellisia asioita kulttuuriosaamisessa, jota kansainvälistyvässä maailmassa arvostetaan yhä enemmän. Tämän opinnäytetyön tavoitteena oli tuottaa selvitys toimeksiantajayrityksen työntekijöiden mielip...

  18. Unity-pelimoottorin hyödyntäminen CAVE-järjestelmässä

    OpenAIRE

    Jussila, Asmo

    2013-01-01

    Opinnäytetyö toteutettiin Seinäjoen ammattikorkeakoulun Tekniikan yksikön virtuaalilaboratorion CAVE-tilaan. Työn tarkoituksena oli uudistaa CAVE:ssa käytössä olevia vanhahtavia virtuaalitodellisuusohjelmia. Työssä selvitettiin kolmen eri pelimoottorin soveltuvuutta CAVE-järjestelmään. Työn pelimoottorivertailuun valittiin suositut ja ilmaiseksi saatavilla olevat Cryengine 3 SDK, Unreal Development Kit sekä Unity-pelimoottorit. Pelimoottoreita vertailtiin keskenään graafisten ominaisuuksi...

  19. Effect of zinc and copper additions on catalytic response of noble metal alloyed 304 SS in high temperature water

    International Nuclear Information System (INIS)

    The effect of zinc (Zn) and copper (Cu) additions on the catalytic behavior of noble metal alloyed 304 stainless steel (SS) in 288 C water understoichiometric excess hydrogen was studied. It was observed that an increase in the Zn or Cu content of the water increased the electrochemical corrosion potential (ECP) of noble metal alloyed 304 SS by ∼ 30 to 50 mV and decreased the recombination efficiency of oxygen (O2)and hydrogen (H2) by ∼ 10%. The change in the ECP and recombination rate was correlated with incorporation of zinc and copper in the oxide film, which, by covering catalytic sites, would alter the redox reaction rate

  20. Corrosion, haemocompatibility and bacterial adhesion behaviour of TiZrN-coated 316L SS for bioimplants

    Indian Academy of Sciences (India)

    Gobi Saravanan Kaliaraj; Vinita Vishwakarma; Ananthakumar Ramadoss; D Ramachandran; Arul Maximus Rabel

    2015-08-01

    TiZrN coating was deposited on 316L stainless steel (SS) by the reactive magnetron co-sputtering technique. Cubic phase of TiZrN with uniform surface morphology was observed by X-ray diffraction and atomic force microscopy. Bacterial adhesion, haemocompatibility and corrosion behaviour of TiZrN coating were examined in order to evaluate the coating’s compatibility for ideal implant. Results revealed that TiZrN coatings exhibited less bacterial attachment against Staphylococcus aureus and Escherichia coli bacteria, negligible platelets activation and superior corrosion resistance than the uncoated 316L SS.

  1. Kuvista musiikiksi : jäsennetty kuvien käyttö 5-vuotiaiden musiikkileikkikouluryhmässä

    OpenAIRE

    Aaltonen, Linda

    2013-01-01

    Opinnäytetyössäni tutkin sitä, miten jäsennetty kuvien käyttö 5-vuotiaiden musiikkileikkikouluryhmässä vaikuttaa lasten keskittymiseen tunnilla. Lisäksi halusin selvittää, miten lapset itse kokivat tunnin ennakoitavuuden. Tutkimuskertoja oli kolme. Ensimmäisellä tunnilla en käyttänyt kuvia tunnin jäsentämiseen, ja kahdella jälkimmäisellä kerralla jäsensin tunnin viiden kuvan kuvasarjalla. Ensimmäisen ja viimeisen kerran lopuksi haastattelin lapsia saadakseni selville heidän mielipiteensä ...

  2. SS7型电力机车电机磁化曲线方程的研究

    Institute of Scientific and Technical Information of China (English)

    吕洋; 刘明光; 李娜

    2007-01-01

    本文首先介绍了W18型硅钢片磁化特性的曲线拟合算法,并根据其算法求得其函数表达式;随后运用Matlad软件编程得到SS7型电力机车的磁化特性曲线,为SS7型电力机车电磁暂态分析提供了坚实的基础.

  3. Ss-Sl2, a novel cell wall protein with PAN modules, is essential for sclerotial development and cellular integrity of Sclerotinia sclerotiorum.

    Science.gov (United States)

    Yu, Yang; Jiang, Daohong; Xie, Jiatao; Cheng, Jiasen; Li, Guoqing; Yi, Xianhong; Fu, Yanping

    2012-01-01

    The sclerotium is an important dormant body for many plant fungal pathogens. Here, we reported that a protein, named Ss-Sl2, is involved in sclerotial development of Sclerotinia sclerotiorum. Ss-Sl2 does not show significant homology with any protein of known function. Ss-Sl2 contains two putative PAN modules which were found in other proteins with diverse adhesion functions. Ss-Sl2 is a secreted protein, during the initial stage of sclerotial development, copious amounts of Ss-Sl2 are secreted and accumulated on the cell walls. The ability to maintain the cellular integrity of RNAi-mediated Ss-Sl2 silenced strains was reduced, but the hyphal growth and virulence of Ss-Sl2 silenced strains were not significantly different from the wild strain. Ss-Sl2 silenced strains could form interwoven hyphal masses at the initial stage of sclerotial development, but the interwoven hyphae could not consolidate and melanize. Hyphae in these interwoven bodies were thin-walled, and arranged loosely. Co-immunoprecipitation and yeast two-hybrid experiments showed that glyceraldehyde-3-phosphate dehydrogenase (GAPDH), Woronin body major protein (Hex1) and elongation factor 1-alpha interact with Ss-Sl2. GAPDH-knockdown strains showed a similar phenotype in sclerotial development as Ss-Sl2 silenced strains. Hex1-knockdown strains showed similar impairment in maintenance of hyphal integrity as Ss-Sl2 silenced strains. The results suggested that Ss-Sl2 functions in both sclerotial development and cellular integrity of S. sclerotiorum.

  4. Kinetic and thermodynamic analysis of the conformational folding process of SS-reduced bovine pancreatic ribonuclease A using a selenoxide reagent with high oxidizing ability.

    Science.gov (United States)

    Arai, Kenta; Kumakura, Fumio; Iwaoka, Michio

    2012-01-01

    Redox-coupled folding pathways of bovine pancreatic ribonuclease A (RNase A) with four intramolecular disulfide (SS) bonds comprise three phases: (I) SS formation to generate partially oxidized intermediate ensembles with no rigid folded structure; (II) SS rearrangement from the three SS intermediate ensemble (3S) to the des intermediates having three native SS linkages; (III) final oxidation of the last native SS linkage to generate native RNase A. We previously demonstrated that DHS(ox), a water-soluble selenoxide reagent for rapid and quantitative SS formation, allows clear separation of the three folding phases. In this study, the main conformational folding phase (phase II) has been extensively analyzed at pH 8.0 under a wide range of temperatures (5-45 °C), and thermodynamic and kinetic parameters for the four des intermediates were determined. The free-energy differences (ΔG) as a function of temperature suggested that the each SS linkage has different thermodynamic and kinetic roles in stability of the native structure. On the other hand, comparison of the rate constants and the activation energies for 3S → des with those reported for the conformational folding of SS-intact RNase A suggested that unfolded des species (desU) having three native SS linkages but not yet being folded are involved in very small amounts (SS-intact RNase A. It was revealed that DHS(ox) is useful for kinetic and thermodynamic analysis of the conformational folding process on the oxidative folding pathways of SS-reduced proteins.

  5. A novel pathogenesis-related protein (SsPR10) from Solanum surattense with ribonucleolytic and antimicrobial activity is stress- and pathogen-inducible.

    Science.gov (United States)

    Liu, Xiaojun; Huang, Beibei; Lin, Juan; Fei, Jiong; Chen, Zhonghai; Pang, Yongzhen; Sun, Xiaofen; Tang, Kexuan

    2006-03-01

    A cDNA clone (designated as SsPR10, GenBank Accession Number AY660753 ) encoding a PR10 protein from yellow-fruit nightshade (Solanum surattense) was isolated and characterized. SsPR10 encoded a 160-amino-acid polypeptide with a predicted molecular mass of 17.58 kDa and pI of 5.29. Sequence alignments showed that SsPR10 had high identity (68.1%) with CaPR10, but had only about 31.7% identity with JIOsPR10 at the amino acid level. Genomic DNA gel blot analysis indicated that SsPR10 belonged to a multigene family. The constitutively expressed SsPR10 was detected to be the highest in roots of the sterile seedlings cultured in jars, while SsPR10 expression was the highest in old yellow leaves from the seedlings incubated with sap containing TMV. SsPR10 always expressed at slightly higher level in senescent leaves than in tender ones under both conditions. Further expression analysis revealed that the signaling components of defense/stress pathways (MeJA, SA, ABA, GA3, H2O2 and Cu2+) up-regulated significantly the SsPR10 mRNA levels over the control. However, darkness failed to induce SsPR10 expression and its expression was also inhibited by cold treatment. The SsPR10 was successfully expressed in Eschericha coli and the expressed protein was purified to near homogeneity. The dialytically renatured SsPR10 protein without phosphorylation exhibited ribonucleolytic activity against S. surattense leaf total RNA preparations and could inhibit hyphal growth of Pyricularia oryzae. Our findings suggest that the novel stress- and pathogen-inducible SsPR10 with ribonucleolytic and antimicrobial activity participates not only in the defense/stress response pathways but also in plants' growth, development and senescence. PMID:16473659

  6. Ss-Sl2, a novel cell wall protein with PAN modules, is essential for sclerotial development and cellular integrity of Sclerotinia sclerotiorum.

    Directory of Open Access Journals (Sweden)

    Yang Yu

    Full Text Available The sclerotium is an important dormant body for many plant fungal pathogens. Here, we reported that a protein, named Ss-Sl2, is involved in sclerotial development of Sclerotinia sclerotiorum. Ss-Sl2 does not show significant homology with any protein of known function. Ss-Sl2 contains two putative PAN modules which were found in other proteins with diverse adhesion functions. Ss-Sl2 is a secreted protein, during the initial stage of sclerotial development, copious amounts of Ss-Sl2 are secreted and accumulated on the cell walls. The ability to maintain the cellular integrity of RNAi-mediated Ss-Sl2 silenced strains was reduced, but the hyphal growth and virulence of Ss-Sl2 silenced strains were not significantly different from the wild strain. Ss-Sl2 silenced strains could form interwoven hyphal masses at the initial stage of sclerotial development, but the interwoven hyphae could not consolidate and melanize. Hyphae in these interwoven bodies were thin-walled, and arranged loosely. Co-immunoprecipitation and yeast two-hybrid experiments showed that glyceraldehyde-3-phosphate dehydrogenase (GAPDH, Woronin body major protein (Hex1 and elongation factor 1-alpha interact with Ss-Sl2. GAPDH-knockdown strains showed a similar phenotype in sclerotial development as Ss-Sl2 silenced strains. Hex1-knockdown strains showed similar impairment in maintenance of hyphal integrity as Ss-Sl2 silenced strains. The results suggested that Ss-Sl2 functions in both sclerotial development and cellular integrity of S. sclerotiorum.

  7. Ss-Sl2, a novel cell wall protein with PAN modules, is essential for sclerotial development and cellular integrity of Sclerotinia sclerotiorum.

    Science.gov (United States)

    Yu, Yang; Jiang, Daohong; Xie, Jiatao; Cheng, Jiasen; Li, Guoqing; Yi, Xianhong; Fu, Yanping

    2012-01-01

    The sclerotium is an important dormant body for many plant fungal pathogens. Here, we reported that a protein, named Ss-Sl2, is involved in sclerotial development of Sclerotinia sclerotiorum. Ss-Sl2 does not show significant homology with any protein of known function. Ss-Sl2 contains two putative PAN modules which were found in other proteins with diverse adhesion functions. Ss-Sl2 is a secreted protein, during the initial stage of sclerotial development, copious amounts of Ss-Sl2 are secreted and accumulated on the cell walls. The ability to maintain the cellular integrity of RNAi-mediated Ss-Sl2 silenced strains was reduced, but the hyphal growth and virulence of Ss-Sl2 silenced strains were not significantly different from the wild strain. Ss-Sl2 silenced strains could form interwoven hyphal masses at the initial stage of sclerotial development, but the interwoven hyphae could not consolidate and melanize. Hyphae in these interwoven bodies were thin-walled, and arranged loosely. Co-immunoprecipitation and yeast two-hybrid experiments showed that glyceraldehyde-3-phosphate dehydrogenase (GAPDH), Woronin body major protein (Hex1) and elongation factor 1-alpha interact with Ss-Sl2. GAPDH-knockdown strains showed a similar phenotype in sclerotial development as Ss-Sl2 silenced strains. Hex1-knockdown strains showed similar impairment in maintenance of hyphal integrity as Ss-Sl2 silenced strains. The results suggested that Ss-Sl2 functions in both sclerotial development and cellular integrity of S. sclerotiorum. PMID:22558105

  8. Ectopic overexpression of SsCBF1, a CRT/DRE-binding factor from the nightshade plant Solanum lycopersicoides, confers freezing and salt tolerance in transgenic Arabidopsis.

    Directory of Open Access Journals (Sweden)

    Lili Zhang

    Full Text Available The C-repeat (CRT/dehydration-responsive element (DRE binding factor (CBF/DREB1 transcription factors play a key role in cold response. However, the detailed roles of many plant CBFs are far from fully understood. A CBF gene (SsCBF1 was isolated from the cold-hardy plant Solanum lycopersicoides. A subcellular localization study using GFP fusion protein indicated that SsCBF1 is localized in the nucleus. We delimited the SsCBF1 transcriptional activation domain to the C-terminal segment comprising amino acid residues 193-228 (SsCBF1(193-228. The expression of SsCBF1 could be dramatically induced by cold, drought and high salinity. Transactivation assays in tobacco leaves revealed that SsCBF1 could specifically bind to the CRT cis-elements in vivo to activate the expression of downstream reporter genes. The ectopic overexpression of SsCBF1 conferred increased freezing and high-salinity tolerance and late flowering phenotype to transgenic Arabidopsis. RNA-sequencing data exhibited that a set of cold and salt stress responsive genes were up-regulated in transgenic Arabidopsis. Our results suggest that SsCBF1 behaves as a typical CBF to contribute to plant freezing tolerance. Increased resistance to high-salinity and late flowering phenotype derived from SsCBF1 OE lines lend more credence to the hypothesis that plant CBFs participate in diverse physiological and biochemical processes related to adverse conditions.

  9. MECHANICAL PROPERTY RESEARCH OF sPS/PET/SsPS/MTA COMPOSITES%sPS/PET/SsPS/MTA复合材料的力学性能研究

    Institute of Scientific and Technical Information of China (English)

    王进; 祝方明; 林尚安

    2002-01-01

    用保持正常熔体插层法制备了新型的间规聚苯乙烯(sPS)/聚对苯二甲酸乙二酯(PET)/磺化间规聚苯乙烯(SsPS-H)及其锌盐(SsPS-Zn)/11-氨基酸改性蒙脱土(MTA)复合材料,该材料的综合力学性能较未加MTA材料显著提高.加入2份MTA时,sPS/PET/SsPS-H/MTA(质量比为85/15/4/2)和sPS/PET/SsPS-Zn/MTA(质量比为85/15/2/2)复合材料的冲击强度达到最大值,分别为15.6 kJ/m2和14.7 kJ/m2,约是纯sPS的3.5倍,是未加MTA材料的1.5倍.拉伸强度和弯曲强度比未填充材料都提高了约30 MPa.

  10. The structure of the XPF-ssDNA complex underscores the distinct roles of the XPF and ERCC1 helix- hairpin-helix domains in ss/ds DNA recognition.

    Science.gov (United States)

    Das, Devashish; Folkers, Gert E; van Dijk, Marc; Jaspers, Nicolaas G J; Hoeijmakers, Jan H J; Kaptein, Robert; Boelens, Rolf

    2012-04-01

    Human XPF/ERCC1 is a structure-specific DNA endonuclease that nicks the damaged DNA strand at the 5' end during nucleotide excision repair. We determined the structure of the complex of the C-terminal domain of XPF with 10 nt ssDNA. A positively charged region within the second helix of the first HhH motif contacts the ssDNA phosphate backbone. One guanine base is flipped out of register and positioned in a pocket contacting residues from both HhH motifs of XPF. Comparison to other HhH-containing proteins indicates a one-residue deletion in the second HhH motif of XPF that has altered the hairpin conformation, thereby permitting ssDNA interactions. Previous nuclear magnetic resonance studies showed that ERCC1 in the XPF-ERCC1 heterodimer can bind dsDNA. Combining the two observations gives a model that underscores the asymmetry of the human XPF/ERCC1 heterodimer in binding at an ss/ds DNA junction.

  11. The pathogenicity factor HrpF interacts with HrpA and HrpG to modulate type III secretion system (T3SS) function and t3ss expression in Pseudomonas syringae pv. averrhoi.

    Science.gov (United States)

    Huang, Yi-Chiao; Lin, Yuan-Chuen; Wei, Chia-Fong; Deng, Wen-Ling; Huang, Hsiou-Chen

    2016-09-01

    To ensure the optimal infectivity on contact with host cells, pathogenic Pseudomonas syringae has evolved a complex mechanism to control the expression and construction of the functional type III secretion system (T3SS) that serves as a dominant pathogenicity factor. In this study, we showed that the hrpF gene of P. syringae pv. averrhoi, which is located upstream of hrpG, encodes a T3SS-dependent secreted/translocated protein. Mutation of hrpF leads to the loss of bacterial ability on elicitation of disease symptoms in the host and a hypersensitive response in non-host plants, and the secretion or translocation of the tested T3SS substrates into the bacterial milieu or plant cells. Moreover, overexpression of hrpF in the wild-type results in delayed HR and reduced t3ss expression. The results of protein-protein interactions demonstrate that HrpF interacts directly with HrpG and HrpA in vitro and in vivo, and protein stability assays reveal that HrpF assists HrpA stability in the bacterial cytoplasm, which is reduced by a single amino acid substitution at the 67th lysine residue of HrpF with alanine. Taken together, the data presented here suggest that HrpF has two roles in the assembly of a functional T3SS: one by acting as a negative regulator, possibly involved in the HrpSVG regulation circuit via binding to HrpG, and the other by stabilizing HrpA in the bacterial cytoplasm via HrpF-HrpA interaction prior to the secretion and formation of Hrp pilus on the bacterial surface. PMID:26638129

  12. Dynamic rockfall risk assessment along the SS113 coastal road (Northern Sicily)

    Science.gov (United States)

    Mastrolembo, V. Brunella; Penna, Ivanna; Voumard, Jérémie; Jaboyedoff, Michel

    2016-04-01

    Rockfalls are natural hazards that usually affect only small areas. However, due to the big amount of material that can be moved and the associated kinetic energy they can cost serious damages to infrastructures and people. Even fairly small rockfall fragments are a significant hazard if deposited on a highway or along a rail-road track since they are not easily detected and can cause accidents or derailments. Rockfalls can also cause the closure of streets resulting in long term indirect economic losses due to transportation delays as well as to the impact on the commercial and tourist activities. In letterature there are numerous examples of rockfall risk assessments along transportation corridors, most of them are based on the use of standard risk estimation methods. The latters are static approaches founded on a macroscopic view of road traffic, assumed as uniform in space and time, thus characterized by average values of parameters. Lately, a new dynamic approach has been developed within the 'risk analysis group' at the University of Lausanne (Voumard, 2013). It consists of a kinematic interpretation of road traffic where vehicles are parametrized as single entities with different characteristics, speed, dimensions and behaviour. We apply this new approach to estimate the dynamic risk due to rockfall occurrence on the SS113 national road running along the northern coast of Sicily. In this work we focus our attention on a ≈10 km section along which the SS113 road and a railway connect all the costal villages going through very steep cliffs and very close to the sea with evident problems of erosion and maintenance. The area is a tourist destination and many hotels and facilities are found along the road. Moreover the area was already hit in the past by numerous rockfalls resulting in the closure of the road for periods running from a few days up to a few years with big direct and indirect damages to the local socio-economic activities. In order to achieve a

  13. Validation of the flow-through chamber (FTC) and steady-state (SS) methods for clearance rate measurements in bivalves

    DEFF Research Database (Denmark)

    Larsen, Poul Scheel; Riisgård, Hans Ulrik

    2012-01-01

    of particles cleared is in itself neither a criterion for valid CR measurement, nor an indicator of appropriate ‘chamber geometry’ as hitherto adapted in many studies. For the ‘steady-state method’ (SS), the design must ensure that inflow water becomes fully mixed with the bivalve’s excurrent flow to establish...... measurements depends on the percent reduction in particle concentration (1006P) from inlet to outlet of the ideal ‘chamber geomety’. For FTC, it decreases with increasing values of P while for SS it first decreases but then increases again, suggesting the use of an intermediate value of P. In practice......, the optimal value of P may depend on the given ‘chamber geometry’. The fundamental differences between the FTC and the SS methods and practical guidelines for their use are pointed out, and new data on CR for the blue mussel, Mytilus edulis, illustrate a design and use of the SS method which may be employed...

  14. Wehrmacht ja relva-SS - rahvaste sõpruse kool / Jüri Kotšinev

    Index Scriptorium Estoniae

    Kotšinev, Jüri, 1960-

    2009-01-01

    Teises maailmasõjas Saksa poolel sõdinud rahvastest, kelle hulgas oli palju NSV Liitu kuulunud rahvaid. Kasakavägedest Saksa sõjaväes ja belglasest Léon Degrellest, kellest sai enim austust pälvinud Euroopa Relva-SS vabatahtlik

  15. Chirality-Selective Photoluminescence Enhancement of ssDNA-Wrapped Single-Walled Carbon Nanotubes Modified with Gold Nanoparticles.

    Science.gov (United States)

    Yang, Juan; Zhao, Qinghua; Lyu, Min; Zhang, Zhenyu; Wang, Xiao; Wang, Meng; Gao, Zhou; Li, Yan

    2016-06-01

    In this work, a convenient method to enhance the photoluminescence (PL) of single-walled carbon nanotubes (SWNTs) in aqueous solutions is provided. Dispersing by single-stranded DNA (ssDNA) and modifying with gold nanoparticles (AuNPs), about tenfold PL enhancement of the SWNTs is observed. More importantly, the selective PL enhancement is achieved for some particular chiralities of interest over all other chiralities, by using certain specific ssDNA sequences that are reported to recognize these particular chiralities. By forming AuNP-DNA-SWNT nanohybrids, ssDNA serves as superior molecular spacers that on one hand protect SWNT from direct contacting with AuNP and causing PL quench, and on the other hand attract the AuNP in close proximity to the SWNT to enhance its PL. This PL enhancement method can be utilized for the PL analysis of SWNTs in aqueous solutions, for biomedical imaging, and may serve as a prescreening method for the recognition and separation of single chirality SWNTs by ssDNA. PMID:27128378

  16. Diagnosis of gallbladder cancer by imaging techniques. Problems, limitations, and their explanations, especially with ss invasive cancer

    Energy Technology Data Exchange (ETDEWEB)

    Tomita, Masao; Onoyama, Hirohiko; Sako, Tatsuo; Ajiki, Tetsuo; Ohara, Sigetsugu; Yamazaki, Iwao; Yamamoto, Masahiro; Saito, Yoichi (Kobe Univ. (Japan). School of Medicine)

    1994-11-01

    The preoperative diagnosis of less than ss depth gallbladder cancer is difficult. Its preoperative diagnosis rate was low (27.5%), and even lower with the presence of concomitant gallstone. In the latter case,the diagnosis rate was particularly low when the stones diameter was greater than 1 cm. Gallstones are frequently associated with macromorphologically invasive type of gallbladder cancer and this may be the reason for the lower diagnostic rate. We compared preoperative diagnosis rate of ultrasonography (US), computed tomography (CT) and drip infusion cholecystography (DIC) for each invasion depth. In US, the preoperative diagnosis rate for m depth invasion was 6/16 (37.5%), pm depth was 1/13 (7.7%), and ss depth was 14/41 (34.1%). In CT, its rate for m depth was 3/11 (27.3%), pm depth was 1/10 (10.0%), and ss depth was 11/37 (29.7%). In DIC, its rate for m depth was 3/11 (27.3%), pm depth was 1/10 (10.0%), and ss was depth 1/23 (4.3%). None of the currently used imaging techniques were very accurate in diagnosing gallbladder cancer. Thus, during preoperative work up, if one discovers a gallbladder full of stones, stones of greater than 1 cm in diameter, thickened gallbladder wall, or a non visualized gallbladder with DIC, gallbladder cancer must be highly suspected. (author).

  17. Measuring Velocity and Acceleration Using Doppler Shift of a Source with an Example of Jet in SS433

    Indian Academy of Sciences (India)

    Sanjay M. Wagh

    2014-12-01

    We describe here as to how the Doppler shift of a source needs to be used to measure its velocity and acceleration. We also apply this method, as an example here, to spectral lines of the blue-shifted jet in micro-quasar SS433 and discuss the intricacies of these measurements.

  18. Effects of heat treatments on microstructure changes in the interface of Cu/SS316L joint materials

    International Nuclear Information System (INIS)

    Precipitation and dispersion strengthened copper alloys joined with 316L austenitic stainless steel are expected to be heat sink materials in the first wall and divertor of International Thermonuclear Experimental Reactor (ITER) owing to the good thermal conductivity of Cu alloys. In the present study, the effects of heat treatment on microstructural stability in the interface of CuNiBe/SS316L and CuAl25/SS316L have been investigated. In the as-received CuNiBe/SS316L joints, voids were observed at the interface, and in the stainless steel side near the interface. But in the CuAl25/SS316L joints, voids were observed only in the Cu side near the interface. These voids would have a significant effect on the mechanical properties of joints. The results of annealing experiments showed that the microstructures in the interface of both types of joints were thermally stable during annealing at 573 and 673 K for 100 h

  19. Economic impacts of the S.S. Glacier Bay oil spill: Social and economic studies. Technical report (Final)

    International Nuclear Information System (INIS)

    On July 2, 1987, an oil spill occurred in Cook Inlet when the S.S. Glacier Bay hit a submerged obstacle while enroute to Kenai Pipeline Company facilities to offload oil. The 1987 commercial fishery in Cook Inlet was barely underway when the S.S. Glacier Bay oil spill occurred, and the largest salmon return in history was moving up the inlet. The sockeye salmon run alone totaled over 12 million, providing a seasonal catch of 9.25 million salmon. The 1987 sport fishery in Cook Inlet was in mid-season at the time of the spill. The S.S. Glacier Bay oil spill represents an opportunity to study the economic impacts of an oil spill event in Alaska, particularly with regard to commercial fishing impacts and the public costs of cleanup. The report evaluates the existing information on the spill, response measures, and economic impacts, and adds discussions with individuals and groups involved in or affected by the spill to this data base. The report reviewed accounts of the oil spill and its costs; identified types and sources of data, developed protocol, and contacted groups and people for data collection and verification; and described, analyzed, and prepared reports of the economic effects of the S.S. Glacier Bay oil spill

  20. First report of Metarhizium anisopliae IP 46 pathogenicity in adult Anopheles gambiae s.s. and An. arabiensis (Diptera; Culicidae)

    NARCIS (Netherlands)

    Mnyone, L.L.; Russell, T.L.; Lyimo, I.N.; Lwetoijera, D.W.; Kirby, M.J.; Luz, C.

    2009-01-01

    The entomopathogenic fungus Metarhizium anisopliae isolate IP 46, originating from a soil sample collected in 2001 in the Cerrado of Central Brazil, was tested for its ability to reduce the survival of adult male and female Anopheles gambiae s.s. and An. arabiensis mosquitoes. A 6-h exposure to the

  1. Highlights from the Functional Single Nucleotide Polymorphisms Associated with Human Muscle Size and Strength or FAMuSS Study

    Directory of Open Access Journals (Sweden)

    Linda S. Pescatello

    2013-01-01

    Full Text Available The purpose of the Functional Single Nucleotide Polymorphisms Associated with Human Muscle Size and Strength study or FAMuSS was to identify genetic factors that dictated the response of health-related fitness phenotypes to resistance exercise training (RT. The phenotypes examined were baseline muscle strength and muscle, fat, and bone volume and their response to RT. FAMuSS participants were 1300 young (24 years, healthy men (42% and women (58% that were primarily of European-American descent. They were genotyped for ~500 polymorphisms and completed the Paffenbarger Physical Activity Questionnaire to assess energy expenditure and time spent in light, moderate, and vigorous intensity habitual physical activity and sitting. Subjects then performed a 12-week progressive, unilateral RT program of the nondominant arm with the dominant arm used as a comparison. Before and after RT, muscle strength was measured with the maximum voluntary contraction and one repetition maximum, while MRI measured muscle, fat, and bone volume. We will discuss the history of how FAMuSS originated, provide a brief overview of the FAMuSS methods, and summarize our major findings regarding genotype associations with muscle strength and size, body composition, cardiometabolic biomarkers, and physical activity.

  2. Polymorphisms rs12998 and rs5780218 in KiSS1 Suppressor Metastasis Gene in Mexican Patients with Breast Cancer

    Directory of Open Access Journals (Sweden)

    Edhit Guadalupe Cruz Quevedo

    2015-01-01

    Full Text Available Aims. KiSS1 is a metastasis suppressor gene associated with inhibition of cellular chemotaxis and invasion attenuating the metastasis in melanoma and breast cancer cell lines. Along the KiSS-1 gene at least 294 SNPs have been described; however the association of these polymorphisms as genetic markers for metastasis in breast cancer studies has not been investigated. Here we describe two simple PCR-RFLPs protocols to identify the rs5780218 (9DelT and the rs12998 (E20K KiSS1 polymorphisms and the allelic, genotypic, and haplotypic frequencies in Mexican general population (GP and patients with benign breast disease (BBD or breast cancer (BC. Results. The rs5780218 polymorphism was individually associated with breast cancer (P=0.0332 and the rs12998 polymorphism shows statistically significant differences when GP versus case (BC and BBD groups were compared (P<0.0001. The H1 Haplotype (G/- occurred more frequently in BC group (0.4256 whereas H2 haplotype (G/T was the most prevalent in BBD group (0.4674. Conclusions. Our data indicated that the rs5780218 polymorphism individually confers susceptibility for development of breast cancer in Mexican population and a possible role as a genetic marker in breast cancer metastasis for H1 haplotype (Wt/variant in KiSS1 gene must be analyzed in other populations.

  3. Structural insights of the ssDNA binding site in the multifunctional endonuclease AtBFN2 from Arabidopsis thaliana.

    Directory of Open Access Journals (Sweden)

    Tsung-Fu Yu

    Full Text Available The multi S1/P1 nuclease AtBFN2 (EC 3.1.30.1 encoded by the Arabidopsis thaliana At1g68290 gene is a glycoprotein that digests RNA, ssDNA, and dsDNA. AtBFN2 depends on three zinc ions for cleaving DNA and RNA at 3'-OH to yield 5'-nucleotides. In addition, AtBFN2's enzymatic activity is strongly glycan dependent. Plant Zn(2+-dependent endonucleases present a unique fold, and belong to the Phospholipase C (PLC/P1 nuclease superfamily. In this work, we present the first complete, ligand-free, AtBFN2 crystal structure, along with sulfate, phosphate and ssDNA co-crystal structures. With these, we were able to provide better insight into the glycan structure and possible enzymatic mechanism. In comparison with other nucleases, the AtBFN2/ligand-free and AtBFN2/PO4 models suggest a similar, previously proposed, catalytic mechanism. Our data also confirm that the phosphate and vanadate can inhibit the enzyme activity by occupying the active site. More importantly, the AtBFN2/A5T structure reveals a novel and conserved secondary binding site, which seems to be important for plant Zn(2+-dependent endonucleases. Based on these findings, we propose a rational ssDNA binding model, in which the ssDNA wraps itself around the protein and the attached surface glycan, in turn, reinforces the binding complex.

  4. SS316 structure fabricated by selective laser melting and integrated with strain isolated optical fiber high temperature sensor

    Science.gov (United States)

    Mathew, Jinesh; Havermann, Dirk; Polyzos, Dimitrios; MacPherson, William N.; Hand, Duncan P.; Maier, Robert R. J.

    2015-09-01

    Smart metal having integrated high temperature sensing capability is reported. The SS316 structure is made by additive layer manufacturing via selective laser melting (SLM). Sensor component is embedded in to the structure during the SLM build process. The strain isolated in-fiber Fabry-Perot cavity sensor measures temperature up to 1100 °C inside the metal.

  5. Structure of the hDmc1-ssDNA filament reveals the principles of its architecture.

    Directory of Open Access Journals (Sweden)

    Andrei L Okorokov

    Full Text Available In eukaryotes, meiotic recombination is a major source of genetic diversity, but its defects in humans lead to abnormalities such as Down's, Klinefelter's and other syndromes. Human Dmc1 (hDmc1, a RecA/Rad51 homologue, is a recombinase that plays a crucial role in faithful chromosome segregation during meiosis. The initial step of homologous recombination occurs when hDmc1 forms a filament on single-stranded (ss DNA. However the structure of this presynaptic complex filament for hDmc1 remains unknown. To compare hDmc1-ssDNA complexes to those known for the RecA/Rad51 family we have obtained electron microscopy (EM structures of hDmc1-ssDNA nucleoprotein filaments using single particle approach. The EM maps were analysed by docking crystal structures of Dmc1, Rad51, RadA, RecA and DNA. To fully characterise hDmc1-DNA complexes we have analysed their organisation in the presence of Ca2+, Mg2+, ATP, AMP-PNP, ssDNA and dsDNA. The 3D EM structures of the hDmc1-ssDNA filaments allowed us to elucidate the principles of their internal architecture. Similar to the RecA/Rad51 family, hDmc1 forms helical filaments on ssDNA in two states: extended (active and compressed (inactive. However, in contrast to the RecA/Rad51 family, and the recently reported structure of hDmc1-double stranded (ds DNA nucleoprotein filaments, the extended (active state of the hDmc1 filament formed on ssDNA has nine protomers per helical turn, instead of the conventional six, resulting in one protomer covering two nucleotides instead of three. The control reconstruction of the hDmc1-dsDNA filament revealed 6.4 protein subunits per helical turn indicating that the filament organisation varies depending on the DNA templates. Our structural analysis has also revealed that the N-terminal domain of hDmc1 accomplishes its important role in complex formation through domain swapping between adjacent protomers, thus providing a mechanistic basis for coordinated action of hDmc1 protomers

  6. Direct imaging of RecA nucleation and growth on single molecules of SSB-coated ssDNA.

    Science.gov (United States)

    Bell, Jason C; Plank, Jody L; Dombrowski, Christopher C; Kowalczykowski, Stephen C

    2012-11-01

    Escherichia coli RecA is the defining member of a ubiquitous class of DNA strand-exchange proteins that are essential for homologous recombination, a pathway that maintains genomic integrity by repairing broken DNA. To function, filaments of RecA must nucleate and grow on single-stranded DNA (ssDNA) in direct competition with ssDNA-binding protein (SSB), which rapidly binds and continuously sequesters ssDNA, kinetically blocking RecA assembly. This dynamic self-assembly on a DNA lattice, in competition with another protein, is unique for the RecA family compared to other filament-forming proteins such as actin and tubulin. The complexity of this process has hindered our understanding of RecA filament assembly because ensemble measurements cannot reliably distinguish between the nucleation and growth phases, despite extensive and diverse attempts. Previous single-molecule assays have measured the nucleation and growth of RecA--and its eukaryotic homologue RAD51--on naked double-stranded DNA and ssDNA; however, the template for RecA self-assembly in vivo is SSB-coated ssDNA. Using single-molecule microscopy, here we directly visualize RecA filament assembly on single molecules of SSB-coated ssDNA, simultaneously measuring nucleation and growth. We establish that a dimer of RecA is required for nucleation, followed by growth of the filament through monomer addition, consistent with the finding that nucleation, but not growth, is modulated by nucleotide and magnesium ion cofactors. Filament growth is bidirectional, albeit faster in the 5'→3' direction. Both nucleation and growth are repressed at physiological conditions, highlighting the essential role of recombination mediators in potentiating assembly in vivo. We define a two-step kinetic mechanism in which RecA nucleates on transiently exposed ssDNA during SSB sliding and/or partial dissociation (DNA unwrapping) and then the RecA filament grows. We further demonstrate that the recombination mediator protein pair

  7. On the Nature of the Compact Object in SS 433. Observational Evidence of X-Ray Photon Index Saturation

    Science.gov (United States)

    Seifina, Elena; Titarchuk, Lev

    2010-01-01

    We present an analysis of the X-ray spectral properties observed from black hole , candidate (BHC) binary SS 433. We have analyzed Rossi X-ray Time Explorer (RXTE) data from this source, coordinated with Green Bank Interferometer/RATAN-600. We show that SS 433 undergoes a X-ray spectral transition from the low hard state (LHS) to the intermediate state (IS). We show that the X-ray broad-band energy spectra during all spectral states are well fit by a sum of so called "Bulk Motion Comptonization (BMC) component" and by two (broad and narrow) Gaussians for the continuum and line emissions respectively. In addition to these spectral model components we also find a strong feature that we identify as a" blackbody-like (BB)" component which color temperature is in the range of 4-5 keV in 24 IS spectra during the radio outburst decay in SS 433. Our observational results on the "high temperature BB" bump leads us to suggest the presence of gravitationally redshifted annihilation line emission in this source. In fact this spectral feature has been recently reproduced in Monte Carlo simulations by Laurent and Titarchuk. We have also established the photon index saturation at about 2.3 in index vs mass accretion correlation. This index-mass accretion correlation allows us to evaluate the low limit of black hole (BH) mass of compact object in SS 433, M(sub bh) approximately > 2 solar masses, using the scaling method using BHC GX 339-4 as a reference source. Our estimate of the BH mass in SS 433 is consistent with recent BH mass measurement using the radial-velocity measurements of the binary system by Hillwig & Gies who find that M(sub x)( = (4.3 +/- 0.8) solar masses. This is the smallest BH mass found up to now among all BH sources. Moreover, the index saturation effect versus mass accretion rate revealed in SS 433, like in a number of other BH candidates, is the strong observational evidence for the presence of a BH in SS 433.

  8. Creep-fatigue evaluation on butt welded joints of type 304 SS

    International Nuclear Information System (INIS)

    In high temperature plant systems such as thermal power generation and petro-chemical complexes, various weldments are used for pressure vessels and piping. Usually in structural design methods for weldment of conventional pressure vessels and piping systems, load controlled stresses are dominant, and to protect early failure in weldments tensile and creep strengths of each welded joint have not to be lower than the standard values of base metal. While under cyclic deformation controlled stress such as thermal loading in LMFBR, creep-fatigue phenomenon caused by reversal loading and residual stress relaxation is dominant. Therefore, a creep-fatigue evaluation method of Type 304 ss butt welded joints has to be investigated under cyclic thermal loading. For a study of this effect, the strain concentration factor Kε; Kε = Max[1+(qwq-1)(1-2 γyσy/E εn), Kε0], which is derived from the generalized elastic follow-up concept as the plastic strain redistribution, was applied, and in the Kε factor the metallurgical discontinuity effects; qw and γy were considered. The elastic follow-up increment factor for weldment; qw is equal to 2 under uni-axial mechanical loading. And under bi-axial thermal loading in shell-type structures, it is clarified that 1.5 as the qw value can be applied. As the yield ration γy, the value of 0.8 is the best for Type 304 ss weldment. As for the creep damage estimation, the simplified procedure based on the time fraction rule is discussed, and it is investigated how the creep damage of weldment can be evaluated by using mechanical properties of base metal (design standard values). The initial value of stress relaxation is determined by the cyclic stress-strain relation of base metal and the above Kε value. Stress relaxation during strain hold can be analyzed by using creep strain behaviour of base metal. Finally the allowable creep-fatigue life can be estimated by using the design curves. (author). 8 refs, 14 figs

  9. Characterization of the potent luteinizing hormone-releasing activity of KiSS-1 peptide, the natural ligand of GPR54.

    Science.gov (United States)

    Navarro, V M; Castellano, J M; Fernández-Fernández, R; Tovar, S; Roa, J; Mayen, A; Nogueiras, R; Vazquez, M J; Barreiro, M L; Magni, P; Aguilar, E; Dieguez, C; Pinilla, L; Tena-Sempere, M

    2005-01-01

    Loss-of-function mutations of the gene encoding GPR54, the putative receptor for the KiSS-1-derived peptide metastin, have been recently associated with hypogonadotropic hypogonadism, in both rodents and humans. Yet the actual role of the KiSS-1/GPR54 system in the neuroendocrine control of gonadotropin secretion remains largely unexplored. To initiate such analysis, the effects of KiSS-1 peptide on LH secretion were monitored using in vivo and in vitro settings under different experimental conditions. Central intracerebroventricular administration of KiSS-1 peptide potently elicited LH secretion in vivo over a range of doses from 10 pmol to 1 nmol. The effect of centrally injected KiSS-1 appeared to be mediated via the hypothalamic LHRH. However, no effect of central administration of KiSS-1 was detected on relative LHRH mRNA levels. Likewise, systemic (i.p. and i.v.) injection of KiSS-1 markedly stimulated LH secretion. This effect was similar in terms of maximum response to that of central administration of KiSS-1 and might be partially attributed to its ability to stimulate LH secretion directly at the pituitary. Finally, the LH-releasing activity of KiSS-1 was persistently observed after blockade of endogenous excitatory amino acid and nitric oxide pathways, i.e. relevant neurotransmitters in the neuroendocrine control of LH secretion. In summary, our results provide solid evidence for a potent stimulatory effect of KiSS-1 on LH release, acting at central levels (likely the hypothalamus) and eventually at the pituitary, and further document a novel role of the KiSS-1/GPR54 system as a relevant downstream element in the neuroendocrine network governing LH secretion. PMID:15375028

  10. KiSS-1基因在黄牛精子发生中的表达%Expression of KiSS-1 gene in bovine testis spermatogenesis

    Institute of Scientific and Technical Information of China (English)

    邵宝平; 王继卿; 罗玉柱; 王建林; 陈建龙; 柴尔青; 徐元青

    2011-01-01

    为探讨亲吻素在黄牛精子发生中的生理功能,并为家畜生殖生理学研究提供参考,运用免疫组织化学SABC染色法并采用Image Pro-Plus 6.0软件,对成年黄牛睾丸中KiSS-1的表达及分布特征进行了研究。结果显示,在成年黄牛睾丸的精原细胞、初级精母细胞、次级精母细胞、早期精子细胞及间质细胞中均发现KiSS-1阳性反应产物,其各阳性细胞积分光密度(IOD)值的大小顺序为IOD初级精母细胞〉IOD间质细胞〉IOD次级精母细胞〉IOD精原细胞〉IOD早期精子细胞,而阳性产物在晚期精子细胞、精子和支持细胞及睾丸间质血管上未被发现;在部分间质细胞周围可见大量的KiSS-1免疫阳性反应产物,而在其他阳性细胞周围未见此现象。结果证实,KiSS-1基因在黄牛精子发生中发挥着重要作用,尤其在精原细胞的有丝分裂和精母细胞的减数分裂中可能发挥着非常重要的作用,而且间质细胞具有向睾丸微环境中分泌KiSS-1的功能。%To explore the physiologic function of the kisspeptins in bovine spermatogenesis as well as provide reference to research reproductive physiology of livestock,kisspeptins-1(Kiss-1) expression characteristics in the testis of adult cattle were studied by using SABC immunohistochemical staining and image analysis software(Image Pro-Plus6.0).In result,the KiSS-1 immunopositive production were found in the spermatogonia,primary spermatocytes,secondary spermatocytes,early spermatids and interstitial cells of the adult bovine testes,with the order of the total optical density of the positive cells(IOD) value as IODprimary spermatocytes IODinterstitial cells IODsecondary spermatocytes〉IODspermatogonia 〉IODearly spermatids(P〈0.05).However,the production were not found in the late spermatids,sperm,sertoli cells and interstitial blood vessels of the adult cattle testes.The large KiSS-1 immunoreactivity production was seen around

  11. Supercritical Accretion Discs in Ultraluminous X-ray Sources and SS 433

    CERN Document Server

    Fabrika, Sergei; Vinokurov, Alexander; Sholukhova, Olga; Shidatsu, Megumi

    2015-01-01

    The black hole mass and accretion rate in Ultraluminous X-ray sources (ULXs) in external galaxies, whose X-ray luminosities exceed those of the brightest black holes in our Galaxy by hundreds and thousands of times$^{1,2}$, is an unsolved problem. Here we report that all ULXs ever spectroscopically observed have about the same optical spectra apparently of WNL type (late nitrogen Wolf-Rayet stars) or LBV (luminous blue variables) in their hot state, which are very scarce stellar objects. We show that the spectra do not originate from WNL/LBV type donors but from very hot winds from the accretion discs with nearly normal hydrogen content, which have similar physical conditions as the stellar winds from these stars. The optical spectra are similar to that of SS 433, the only known supercritical accretor in our Galaxy$^{3}$, although the ULX spectra indicate a higher wind temperature. Our results suggest that ULXs with X-ray luminosities of $\\sim 10^{40}$ erg s$^{-1}$ must constitute a homogeneous class of objec...

  12. Early diagnosis of primary Sjögren's syndrome: EULAR-SS task force clinical recommendations.

    Science.gov (United States)

    Brito-Zerón, Pilar; Theander, Elke; Baldini, Chiara; Seror, Raphaèle; Retamozo, Soledad; Quartuccio, Luca; Bootsma, Hendrika; Bowman, Simon J; Dörner, Thomas; Gottenberg, Jacques-Eric; Mariette, Xavier; Bombardieri, Stefano; de Vita, Salvatore; Mandl, Thomas; Ng, Wan-Fai; Kruize, Aike A; Tzioufas, Athanasios; Vitali, Claudio; Buyon, Jill; Izmirly, Peter; Fox, Robert; Ramos-Casals, Manuel

    2016-01-01

    Sjögren's syndrome (SjS) is a systemic autoimmune disease that mainly affects the exocrine glands, leading to generalized mucosal dryness. However, primary SjS may initially present with non-sicca (systemic) manifestations. When these features appear before the onset of an overt sicca syndrome, we may talk of an underlying 'occult' SjS. The European League Against Rheumatism (EULAR) has promoted and supported an international collaborative study group (EULAR-SS Task Force) aimed at developing consensual recommendations to provide a homogeneous approach to the patient with primary SjS presenting with systemic involvement. This review summarizes the key factors that should be taken into account in the diagnostic approach in a patient with suspected SjS according to the main clinical patterns of presentation, and is especially focused on organ-specific systemic disease presentations, including a consensus set of recommendations in order to reach an early diagnosis. Close collaboration with the different specialties involved through a comprehensive multidisciplinary approach is essential in SjS patients presenting with systemic involvements.

  13. PN Sequence Preestimator Scheme for DS-SS Signal Acquisition Using Block Sequence Estimation

    Directory of Open Access Journals (Sweden)

    Sang Kyu Park

    2005-03-01

    Full Text Available An m-sequence (PN sequence preestimator scheme for direct-sequence spread spectrum (DS-SS signal acquisition by using block sequence estimation (BSE is proposed and analyzed. The proposed scheme consists of an estimator and a verifier which work according to the PN sequence chip clock, and provides not only the enhanced chip estimates with a threshold decision logic and one-chip error correction among the first m received chips, but also the reliability check of the estimates with additional decision logic. The probabilities of the estimator and verifier operations are calculated. With these results, the detection, the false alarm, and the missing probabilities of the proposed scheme are derived. In addition, using a signal flow graph, the average acquisition time is calculated. The proposed scheme can be used as a preestimator and easily implemented by changing the internal signal path of a generally used digital matched filter (DMF correlator or any other correlator that has a lot of sampling data memories for sampled PN sequence. The numerical results show rapid acquisition performance in a relatively good CNR.

  14. Computational approach to analyze isolated ssDNA aptamers against angiotensin II.

    Science.gov (United States)

    Heiat, Mohammad; Najafi, Ali; Ranjbar, Reza; Latifi, Ali Mohammad; Rasaee, Mohammad Javad

    2016-07-20

    Aptamers are oligonucleotides with highly structured molecules that can bind to their targets through specific 3-D conformation. Commonly, not all the nucleotides such as primer binding fixed region and some other sequences are vital for aptamers folding and interaction. Elimination of unnecessary regions needs trustworthy prediction tools to reduce experimental efforts and errors. Here we introduced a manipulated in-silico approach to predict the 3-D structure of aptamers and their target interactions. To design an approach for computational analysis of isolated ssDNA aptamers (FLC112, FLC125 and their truncated core region including CRC112 and CRC125), their secondary and tertiary structures were modeled by Mfold and RNA composer respectively. Output PDB files were modified from RNA to DNA in the discovery studio visualizer software. Using ZDOCK server, the aptamer-target interactions were predicted. Finally, the interaction scores were compared with the experimental results. In-silico interaction scores and the experimental outcomes were in the same descending arrangement of FLC112>CRC125>CRC112>FLC125 with similar intensity. The consistent results of innovative in-silico method with experimental outputs, affirmed that the present method may be a reliable approach. Also, it showed that the exact in-silico predictions can be utilized as a credible reference to find aptameric fragments binding potency. PMID:27188956

  15. Synthesis of calcium hydrogen phosphate and hydroxyapatite coating on SS316 substrate through pulsed electrodeposition.

    Science.gov (United States)

    Chakraborty, Rajib; Sengupta, Srijan; Saha, Partha; Das, Karabi; Das, Siddhartha

    2016-12-01

    The orthopaedic implants for human body are generally made of different biomaterials like stainless steels or Ti based alloys. However, it has been found that from surface properties point of view, none of these materials is attractive for fast tissue or cell growth on the surface of implant. This is one of the most important criteria to assure quick bonding between implant and body tissues vis-à-vis minimum recovery time for the patient. Keeping in view of the above facts, this work involves the pulsed electro-deposition coating of biocompatible hydroxyapatite and its group compounds from a diluted bath of calcium and phosphate salt at various current densities over the biomaterial sheet of SS316. SEM study confirms different morphologies of the coatings at different current densities. Characterization techniques like X-ray diffraction, SEM with EDX and FTIR have been used to confirm the phase and percentage quantity of hydroxyapatite compound in the depositions. This coating can serve as a medium for faster tissue growth over the metallic implants. PMID:27612782

  16. Computaional Modeling of the Stability of Crevice Corrosion of Wetted SS316L

    Energy Technology Data Exchange (ETDEWEB)

    F. Cui; F.J. Presuel-Moreno; R.G. Kelly

    2006-04-17

    The stability of localized corrosion sites on SS 316L exposed to atmospheric conditions was studied computationally. The localized corrosion system was decoupled computationally by considering the wetted cathode and the crevice anode separately and linking them via a constant potential boundary condition at the mouth of the crevice. The potential of interest for stability was the repassivation potential. The limitations on the ability of the cathode that are inherent due to the restricted geometry were assessed in terms of the dependence on physical and electrochemical parameters. Physical parameters studied include temperature, electrolyte layer thickness, solution conductivity, and the size of the cathode, as well as the crevice gap for the anode. The current demand of the crevice was determined considering a constant crevice solution composition that simulates the critical crevice solution as described in the literature. An analysis of variance showed that the solution conductivity and the length of the cathode were the most important parameters in determining the total cathodic current capacity of the external surface. A semi-analytical equation was derived for the total current from a restricted geometry held at a constant potential at one end. The equation was able to reproduce all the model computation results both for the wetted external cathode and the crevice and give good explanation on the effects of physicochemical and kinetic parameters.

  17. Parametric Data Modeling and Experimental Approach for Spot Weldment in SS202

    Directory of Open Access Journals (Sweden)

    S. K.Sahoo

    2013-11-01

    Full Text Available The interest of data modeling of process parameters in now a days based broadly upon numerical methods .variations in parametric set can be experimented for finding an optimized or desired value for a output property. This paper is to expose the experimental design approach to process parameter optimization of spot welding process for SS202.The objective of this experiment is to investigate the welding parameters performance in tensile shear failure strength and to optimize the parametric set for highest tensile strength achievable. From the experimental data achieved, the parametric relation between tensile shear stress and the parameters were found out using regression analysis .The optimum value for the parameters affecting tensile shear strength also found out by using soft computing methods like Simulated Annealing and Genetic Algorithm. There are various types of parameter that influence the mechanical properties of welding on the spot welding machine where in this experiment the parameter that will be investigate are weld cycle, squeeze time and weld current , hold time, weld force and weld current. Here also both optimized values were compared for greatest tensile shear strength value.

  18. New concept on ductility exhaustion considering creep-fatigue failure mechanism of Type 304SS

    International Nuclear Information System (INIS)

    For long-term creep-fatigue of Type 304SS, intergranular failure is dominant when significant life reduction occurs. This phenomenon has its origin in grain boundary sliding as same as that in cavity type creep failure. Accordingly a simplified procedure to estimate intergranular damages caused by grain boundary sliding is presented in connection with the secondary creep. In the conventional ductility exhaustion method, fracture ductility includes plastic strain, and damage estimation is based on primary creep recoverable during strain cycling. Therefore, the accumulated creep strain becomes a very large value, and is quite different from grain boundary sliding strain. As a new concept on ductility exhaustion, the products of secondary creep rate and time to rupture (ε2tR) is applied to fracture ductility, and grain boundary sliding is estimated using the accumulated secondary creep strain. It was shown that the time fraction rule and the conventional ductility exhaustion method can be derived analytically from the new concept. Furthermore an advanced method on cyclic stress relaxation was examined. If cyclic plastic strain hardening is softened thermally during strain hold, softening of the primary and secondary creep can occur. And the unrecoverable accumulated primary creep strain causes hardening of the primary creep. The reduction of deformation resistance to the secondary creep accelerates the grain boundary sliding rate, and increases creep damage. The new concept ductility exhaustion method based on the above consideration gave good life prediction for the intergranular failure mode. (author)

  19. ssDNA-Functionalized Nanoceria: A Redox-Active Aptaswitch for Biomolecular Recognition.

    Science.gov (United States)

    Bülbül, Gonca; Hayat, Akhtar; Andreescu, Silvana

    2016-04-01

    Quantification of biomolecular binding events is a critical step for the development of biorecognition assays for diagnostics and therapeutic applications. This paper reports the design of redox-active switches based on aptamer conjugated nanoceria for detection and quantification of biomolecular recognition. It is shown that the conformational transition state of the aptamer on nanoceria, combined with the redox properties of these particles can be used to create surface based structure switchable aptasensing platforms. Changes in the redox properties at the nanoceria surface upon binding of the ssDNA and its target analyte enables rapid and highly sensitive measurement of biomolecular interactions. This concept is demonstrated as a general applicable method to the colorimetric detection of DNA binding events. An example of a nanoceria aptaswitch for the colorimetric sensing of Ochratoxin A (OTA) and applicability to other targets is provided. The system can sensitively and selectivity detect as low as 0.15 × 10(-9) m OTA. This novel assay is simple in design and does not involve oligonucleotide labeling or elaborate nanoparticle modification steps. The proposed mechanism discovered here opens up a new way of designing optical sensing methods based on aptamer recognition. This approach can be broadly applicable to many bimolecular recognition processes and related applications. PMID:26844813

  20. Fusion welding studies using laser on Ti-SS dissimilar combination

    Science.gov (United States)

    Shanmugarajan, B.; Padmanabham, G.

    2012-11-01

    Laser welding investigations were carried out on dissimilar Ti-SS combination. The study is aimed to improve the weld strength and ductility by minimizing harmful intermetallics and taking advantage of high cooling rates in laser welding. Results of continuous wave 3.5 kW CO2 laser welding of totally dissimilar combination of Titanium and stainless steel (304) have been discussed. Bead on plate welding experiments were conducted to identify the laser welding parameters using depth of penetration as criteria. The welding of dissimilar combination has been attempted both autogenously and with interlayers such as Vanadium (V) and Tantalum (Ta) in the form of laser cladding as well as strip. Autogenous welds were carried out by varying the laser power, welding speed and position of the laser beam with respect to the joint centre. The resultant welds are characterized by macrostructure analysis, SEM/EDAX and XRD and as welded tensile test in UTM. The autogenous welds have exhibited extensive cracking even when welded at high speeds or by manipulating the beam position with respect to the joint. Similarly Vandaium as interlayer could not achieve crack free joint. A joint with 40 MPa strength could be made with Ta as interlayer. Results and analysis of these variants of laser welded joints are reported and discussed.