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Sample records for blastocystis hominis sobre

  1. [Blastocystis hominis and bowel diseases].

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    Ustün, Sebnem; Turgay, Nevin

    2006-01-01

    Blastocystis hominis (B. hominis) is a parasite of uncertain role in human disease. It may be identified during a workup for gastrointestinal symptoms, usually in stools. The clinical consequences of B. hominis infection are mainly diarrhea and abdominal pain as well as nonspecific gastrointestinal symptoms such as nausea, anorexia, vomiting, weight loss, lassitude, dizziness, and flatulence. Case reports and series have suggested a pathogenic role of B. hominis in causing intestinal inflammation. Also some studies have suggested that inflammatory bowel disease (IBD) and irritable bowel syndrome (IBS) are associated with B. hominis infection. The investigators indicate that the stools of all patients presenting with IBD or IBS should be examined, and culture methods for B. hominis carried out. Invasion and mucosal inflammation of the intestine with B. hominis have been observed in studies of gnotobiotic guinea pigs. The transmission, pathogenicity, culture characteristics, taxonomy, life cycle, biochemistry and molecular biology of B. hominis remain unclear. More studies are necessary for this parasite. PMID:17106862

  2. Blastocystis hominis in hospital employees.

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    Grossman, I; Weiss, L M; Simon, D; Tanowitz, H B; Wittner, M

    1992-06-01

    Several reports have appeared that either support or deny the importance of the protozoan Blastocystis hominis as an intestinal pathogen in humans. In this report, we describe the clinical characteristics of B. hominis and its response to therapy in hospital employees found to have the parasite on routine screening of stools. During the study, 49 patients with B. hominis were identified, and 413 stools were examined from these patients. Twenty-nine patients were asymptomatic (59%), and 20 had symptoms of bloating, flatulence, soft/loose stools, or constipation. Of these 20 patients, 10 had symptoms that correlated with the presence or absence of B. hominis, four had symptoms that were independent of B. homonis, and six had other intestinal parasites that could account for their symptoms. Nineteen percent of patients without treatment had eradication of B. hominis from stool on follow-up examination. Metronidazole did not increase this rate. Iodoquinol treatment eradicated the organism in 41% of patients (p less than 0.05), and resulted in the reduction or eradication of the parasite in 62%, as determined by follow-up examination. PMID:1590309

  3. Observações sobre Blastocystis hominis e Cyclospora cayetanensis em exames parasitológicos efetuados rotineiramente Observations on Blastocystis hominis and Cyclospora cayetanensis in routine parasitological examinations

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    Ruth Semira Rodriguez Alarcón

    2007-04-01

    Full Text Available Relatamos algumas observações, efetuadas com exames parasitológicos de fezes, em atividades rotineiras: os métodos de Faust e cols e de sedimentação espontânea em água não servem para evidenciação de Blastocystis hominis; foram encontradas expressivas porcentagens de presença desse protozoário, sobretudo quando realizada coloração pela hematoxilina férrica; houve 0,7% de registro de positividade para Cyclospora cayetanensis, sugerindo inclusão habitual de pesquisa, por técnicas apropriadas, de tal parasita.We report some observations made from routine parasitological examinations on feces. The methods of Faust et al. and of spontaneous sedimentation in water are not enough to identify Blastocystis hominis. Significant percentage presence of this protozoan was found, especially when staining with iron hematoxylin was performed. Cyclospora cayetanensis was found in 0.7% of the cases, which suggests that this parasite should also routinely be investigated by appropriate techniques.

  4. Prevalence and characteristics of Blastocystis hominis infection in children.

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    O'Gorman, M A; Orenstein, S R; Proujansky, R; Wadowsky, R M; Putnam, P E; Kocoshis, S A

    1993-02-01

    Blastocystis hominis, a protozoan whose pathogenicity has been questioned, is sometimes found in the human gastrointestinal tract. We sought to determine the prevalence of Blastocystis in stool and to characterize clinical features of infection with Blastocystis in children. Forty-six (3%) of 1,736 patients undergoing fecal microscopy at Children's Hospital of Pittsburgh between January 1, 1985, and December 31, 1988, harbored Blastocystis. Of these 46 children, 75% had exposure to well water or had been in developing countries. Thirty-nine of the 46 (85%) experienced gastrointestinal symptoms, such as abdominal pain, diarrhea, vomiting, and weight loss. Blastocystis was the only parasite found in 35 of those 39 symptomatic children. Symptoms resolved within one month in 90% of patients receiving antiparasitic pharmacotherapy, but in only 58% (P Blastocystis often experience gastrointestinal symptoms and that treatment increases the rate of symptomatic improvement. We speculate that Blastocystis is a human pathogen. PMID:8432086

  5. Characteristics of Blastocystis hominis infection in a Turkish university hospital.

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    Ozçakir, Olcay; Güreser, Semra; Ergüven, Sibel; Yilmaz, Yakut Akyön; Topaloğlu, Rezzan; Hasçelik, Gülşen

    2007-01-01

    In order to determine characteristics of Blastocystis (B.) hominis infection; 770 individuals' stool specimens were examined both by simple and concentration techniques and stained with iodine solution and trichrome in the Parasitology Laboratory of Hacettepe University Faculty of Medicine, Turkey. Among the examined 770 specimens, B. hominis was detected in 94 (12.2%). B. hominis was the most common intestinal parasite among the study group. It was mostly detected with Dientamoeba fragilis. Among the groups the incidence of B. hominis in allergic patients was higher than controls. Among the immunosuppressed patients, B. hominis was detected significantly higher in patients who had solid tumours. Of the 48 individuals who had only B. hominis in their stool the most common symptom was abdominal pain. Concentration technique with trichrome stain was more sensitive than simple smear with lugol solution for the detection of B. hominis. Studies with more patients must be planed to understand the B. hominis infection in solid tumour patients and coexistence of B. hominis and D. fragilis. PMID:18224616

  6. ARE WE NEGLECTING BLASTOCYSTIS HOMINIS IN PATIENTS HAVING IRRITABLE BOWEL SYNDROME

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    Rashi S

    2015-08-01

    Full Text Available Blastocystis hominis has become unique and an interesting pathogenic parasite and has been reported to contribute to irritable bowel syndrome. The parasite is highly pleomorphic and its diagnostic yield increases when more than one laboratory technique is used. Blastocystis hominis responds to metronidazole and trimethoprim - sulfamethoxa zole (TMP - SMX. The prevalence of Blastocystis hominis in patients having irritable bowel syndrome is reported to be 43% in the present study. Blastocystis hominis was seen in 25%, 30%, 18.5% and 43% samples using wet mount, trichrome staining, formol ethe r sedimentation technique and culture in modified Jones’ medium respectively. Vacuolar forms were the predominant forms. 87.34% patients did not show Blastocystis hominis in stool samples after treatment whereas 12.65% showed the presence of parasite. Hence, it becomes mandatory to examine stool specimen of patients having irritable bowel syndrome for early diagnosis and treatment of these patients will reduce morbidity.

  7. Eradication of Blastocystis hominis prevents the development of symptomatic Hashimoto's thyroiditis: a case report.

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    Rajič, Borko; Arapović, Jurica; Raguž, Kazimir; Bošković, Mladen; Babić, Senaida Marina; Maslać, Suzana

    2015-07-01

    In this case report we describe a 49 year-old man who presented with chronic urticaria, angioedema and soft stool consistency. During diagnostic examinations Hashimoto's thyroiditis was found even though the patient never had clear symptoms of this disease. Blastocystis hominis was isolated through a stool microbiologic examination, implicating that this parasite can cause the development of Hashimoto's thyroiditis and chronic urticaria. After two-weeks treatment with metronidazole the Blastocystis hominis was eradicated, then urticaria and angioedema disappeared. During the four years of follow-up, the patient presented without any symptoms, whereas thyroid hormones were normalized and anti-thyroid antibodies declined. For the first time in the literature we show that eradication of Blastocystis hominis can prevent the development of both symptomatic Hashimoto's thyroiditis and chronic urticaria. PMID:26230132

  8. IN VIVO AND IN VITRO EFFICACY OF NIGELLA SATIVA AQUEOUS EXTRACT ON BLASTOCYSTIS HOMINIS.

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    Eida, Omima M; El-Shafei, Hanaa A; Nomeir, Yousra A; El Safhi, Mohammed B

    2016-04-01

    Metronidazole (MTZ) was the most widely accepted treatment for Blastocystis hominis (B. hominis) with high treatment failure rate, resistance and potential mutagenic and carcinogenic effects so there is urgent need to find out new, effective and safe treatment against B. hominis. The present research aimed to evaluate the therapeutic effect of the aqueous extract of Nigella sativa (NS) at different doses on B. hominis in vitro and in vivo in comparison to MTZ as a control drug. Isolates of B. hominis were obtained from patients complaining of diarrhea and abdominal pain. Isolates were cultured in egg diphasic medium (LE) for in vitro study and to adjust proper inoculating dose for in vivo study. The aqueous extract of NS at concentrations of 100 & 500 µg/ml showed a potent lethal effect on B. hominis isolates in vitro. Caecal tissue of experimentally infected and treated mice with two different doses of NS (250 & 500 mg/kg/d) were examined histopathologically and compared with that of mice infected and treated by two doses of MTZ (62 & 125 mg/kg/d) as control drug and Infected untreated mice as negative control group. Histopathological examination of infected untreated group showed all pathological degrees in the caecal tissue while infected treated one showed remission of pathological changes especially with higher dose (500 mg/kg). Present study proved that N. sativa had inhibitory effect on B. hominis in vitro and prevented cytopathic effect in infected mice inoculated orally with B. hominis. PMID:27363038

  9. Blastocystis hominis and Endolimax nana Co-Infection Resulting in Chronic Diarrhea in an Immunocompetent Male

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    Mitanshu Shah

    2012-06-01

    Full Text Available Blastocystis hominis and Endolimax nana exist as two separate parasitic organisms; however co-infection with the two individual parasites has been well documented. Although often symptomatic in immunocompromised individuals, the pathogenicity of the organisms in immunocompetent subjects causing gastrointestinal symptoms has been debated, with studies revealing mixed results. Clinically, both B. hominis and E. nana infection may result in acute or chronic diarrhea, generalized abdominal pain, nausea, vomiting, flatulence and anorexia. We report the case of a 24-year-old immunocompetent male presenting with chronic diarrhea and abdominal pain secondary to B. hominis and E. nana treated with metronidazole, resulting in symptom resolution and eradication of the organisms. Our case illustrates that clinicians should be cognizant of both B. hominis and E. nana infection as a cause of chronic diarrhea in an immunocompetent host. Such awareness will aid in a timely diagnosis and possible parasitic eradication with resolution of gastrointestinal symptoms.

  10. Morphological diversity of Blastocystis hominis in sodium acetate-acetic acid-formalin-preserved stool samples stained with iron hematoxylin.

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    Macpherson, D. W.; MacQueen, W M

    1994-01-01

    The objective of this investigation was to study the morphological characteristics of Blastocystis hominis in sodium acetate-acetic acid-Formalin-preserved stool samples. Routinely processed samples were examined for morphological detail, including size, shape, nuclear detail, and central body characteristics. Morphological findings revealing the importance of recognizing B. hominis in the diagnostic laboratory are described.

  11. Morphological diversity of Blastocystis hominis in sodium acetate-acetic acid-formalin-preserved stool samples stained with iron hematoxylin.

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    MacPherson, D W; MacQueen, W M

    1994-01-01

    The objective of this investigation was to study the morphological characteristics of Blastocystis hominis in sodium acetate-acetic acid-Formalin-preserved stool samples. Routinely processed samples were examined for morphological detail, including size, shape, nuclear detail, and central body characteristics. Morphological findings revealing the importance of recognizing B. hominis in the diagnostic laboratory are described. PMID:7510311

  12. Clinical significance and frequency of Blastocystis hominis in Turkish patients with hematological malignancy.

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    Taşova Y

    2000-06-01

    Full Text Available The effect of Blastocystis hominis (B. hominis in both immunocompetent and immunocompromised subjects has been the subject of debate in recent years, mostly in response to its unknown pathogenicity and frequency of occurrence. We performed a non-randomised, open labelled, single institute study in our hospital in order to investigate the clinical significance and frequency of B. hominis in patients suffering from hematological malignancy (HM who displayed symptoms of gastrointestinal diseases during the period of chemotherapy-induced neutropenia. The presence and potential role of other intestinal inclusive of parasites were also studied. At least 3 stool samples from each of 206 HM patients with gastrointestinal complaints (the HM group were studied. These were compared with stool samples from a control group of 200 patients without HM who were also suffering from gastrointestinal complaints. Samples were studied with saline-lugol, formalin-ether, and trichome staining methods. Groups were comparable in terms of gender, age and type of gastrointestinal complaints. In the HM group, the most common parasite was B. hominis. In this group, 23 patients (13% had B. hominis, while in the control group only 2 patients (1% had B. hominis. This difference was statistically significant (P < 0.05. Symptoms were non-specific for B. hominis or other parasites in the HM group. The predominant symptoms in both groups were abdominal pain (87-89.5%, diarrhea (70-89.5%, and flatulence (74-68.4%. Although all patients with HM were symptom-free at the end of treatment with oral metranidazol (1,500 mg per day for 10 days 2 patients with HM had positive stool samples containing an insignificant number of parasites (< 5 cells per field. In conclusion, it appears that B. hominis is not rare and should be considered in patients with HM who have gastrointestinal complaints while being treated with chemotherapy. Furthermore, metranidazol appears to be effective in

  13. Stress exacerbates infectivity and pathogenicity of Blastocystis hominis: in vitro and in vivo evidences.

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    Samudi Chandramathi

    Full Text Available BACKGROUND: Stress alters the oxidant-antioxidant state and immune cell responses which disrupts its function to combat infection. Blastocystis hominis, a common intestinal protozoan has been reported to be opportunistic in immunocompromised patients namely cancer. B. hominis infectivity in other altered immune system conditions especially stress is unknown. We aimed to demonstrate the stress effects towards the susceptibility and pathogenicity of B. hominis infection. METHODS/FINDINGS: Three-week-old Wistar rats were divided into four groups: (acontrol; (bstress-induced; (cB. hominis infected; (dstress-induced with B. hominis infection; (n = 20 respectively. Stress was induced for an hour daily (30 days using a Belly Dancer Shaker. Weight gain was monitored, stool samples were collected for B. hominis screening and blood for the determination of differential count, levels of immunoglobulin, oxidative damage, and peripheral blood mononuclear cell (PBMC proliferation upon induction with solubilized antigen of B. hominis (Blasto-Ag. Group (b exhibited the highest level of weight gain. Group (d had higher levels of parasite cyst count in stools, serum IgE, oxidized protein and lipid compared to the group (c. Levels of monocyte and antioxidant in group (d were decreased and their PBMCs showed highest inhibition of proliferation level when exposed to Blasto-Ag. Monocyte level in Group (b showed insignificant difference compared to group (a but was significantly lower compared to group (c. Antioxidant levels in group (c were generally lower compared to group (a and (b. Inhibition level exhibited by Blasto-Ag treated PBMCs of group (c was higher compared to group (a and (b. CONCLUSION: The pathogenicity and augmentation of B. hominis infection is enhanced when stress is present. Lifestyles today are becoming increasingly stressed and the present findings suggest that the parasite which has been reported to be one of the most common organisms seen in

  14. The prevalence of Blastocystis hominis and other protozoan parasites in soldiers returning from peacekeeping missions.

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    Duda, Aleksandra; Kosik-Bogacka, Danuta; Lanocha-Arendarczyk, Natalia; Kołodziejczyk, Lidia; Lanocha, Aleksandra

    2015-04-01

    Blastocystis hominis is a common intestinal parasite found in humans living in poor sanitary conditions, living in tropical and subtropical climates, exposed to infected animals, or consuming contaminated food or water. The aim of this study was to determine the prevalence of B. hominis in Polish military personnel returning from peacekeeping missions in Iraq and Afghanistan. In total, 1,826 stool samples were examined. Gastrointestinal parasites were detected in 17% of the soldiers. The examined stool samples most frequently contained vacuolar forms of B. hominis (15.3%) and cysts of Entamoeba coli (1.0%) or Giardia lamblia (0.7%). In 97.1% of stool samples from infected soldiers, we observed less than five developmental forms of B. hominis in the field of view (40×). The parasite infections in soldiers were diagnosed in the autumn and the spring. There was no statistical correlation between age and B. hominis infection. Our results show that peacekeeping missions in countries with tropical or subtropical climates could be associated with risk for parasitic diseases, including blastocystosis. PMID:25732683

  15. Blastocystis hominis: phylogenetic affinities determined by rRNA sequence comparison.

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    Johnson, A M; Thanou, A; Boreham, P F; Baverstock, P R

    1989-04-01

    In 1912 Blastocystis hominis was identified as a new species and classified as a yeast (Brumpt 1912). In the early 1920s several groups confirmed its classification as a yeast, specifically a member of the genus Schizosaccharomyces (discussed by Zierdt et al. 1967). Apart from an occasional case report, the classification of B. hominis and its role as a harmless intestinal yeast was not questioned for another 50 years. Then, Zierdt (1967) suggested that it should be classified in the phylum Protozoa, subphylum Sporozoa, and that it should be considered as a potential pathogen. The likely role of B. hominis as a human pathogen has recently become more firmly established (Garcia et al. 1984; Sheehan et al. 1986) and its classification has been changed. Although the classification of B. hominis as a protozoon was assumed widely, classification as a sporozoon was not accepted, and the most recent definitive classification of the Protozoa did not even list B. hominis (Lee et al. 1985). Then, based essentially on a review of the known characteristics of the organism, it was recently reclassified into the subphylum Sarcodina (Zierdt 1988). Clearly, the phylogeny of this emerging human pathogen needs definitive analysis (Mehlhorn 1988). PMID:2649390

  16. Clinical characteristics and endoscopic findings associated with Blastocystis hominis in healthy adults.

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    Chen, Te-Li; Chan, Che-Chang; Chen, Hsin-Pai; Fung, Chang-Phone; Lin, Chih-Pei; Chan, Wan-Leong; Liu, Cheng-Yi

    2003-08-01

    Ninety-nine individuals with stools positive for Blastocystis hominis but negative for other parasites were identified from medical records of healthy adults who had received a physical examination at Taipei Veterans General Hospital from November 2000 to October 2002. The medical records of these 99 positive cases and 193 randomly selected controls, matched for age, sex, and date of examination, were retrospectively reviewed. The pathogenicity of B. hominis could not be demonstrated due to a lack of association with the development of gastrointestinal symptoms or pathologic findings on endoscopic examination. Multivariate analyses revealed that chronic hepatitis B infection was a predisposing condition to the acquisition of B. hominis (odd ratio = 2.848, 95% confidence interval = 1.299-6.242, P = 0.009), and concentration of urate was significantly lower in B. hominis-positive individuals (mean +/- SD = 361.64 +/- 87.44 versus 392.57 +/- 93.38 micromol/L; P = 0.009). Among the 64 individuals who underwent gastric biopsy, Helicobacter pylori was found more frequently in the individuals harboring B. hominis (19 of 26 versus 15 of 38; P = 0.017). PMID:13677378

  17. [Blastocystis hominis in patients at the Ruiz y Paez University Hospital from Bolivar City, Venezuela].

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    Devera, R; Azacon, B; Jiménez, M

    1998-01-01

    Blastocystis hominis is a polymorphic protozoan of discussed taxonomic position, which is currently associated with human intestinal disease. In order to determine the prevalence of the microorganism in a sample of hospitalized patients, a study was carried out from november 1996 to april 1997 on 100 adult patients of both sexes aged 20 to 79 years at the "Ruíz y Páez" University Hospital of Bolivar city, Venezuela. A coproparasitological study was carried out using direct examination and Faust method. Infection by parasites and/or commensals was demonstrated in 48 patients. The most frequent agent was B. hominis with a prevalence of 42.0%. We did not find a statistically association between sex (P > 0.05) or age (X2 = 3.52; d.f; = 3) and B. hominis infection. B. hominis was most frequently identified as the single parasite (88.1%), and with a number of less than 5 cells per 400X microscopic field (73.8%). The infection was more common in patients with base chronic-immunosuppressive diseases, the major one being cancer. Diarrhea was observed in 27.0% of cases. Due to its high prevalence, especially as a single agent, together with the particular immunological characteristics of the patients studied, a potential pathogenic role of the opportunistic type is suggested for B. hominis. PMID:10413881

  18. [Prevalence of Blastocystis hominis among food handlers from Caroni municipality, Bolivar State, Venezuela].

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    Requena, Ixora; Hernández, Yessica; Ramsay, Mario; Salazar, Carmen; Devera, Rodolfo

    2003-01-01

    A cross-sectional survey was conducted to determine the prevalence of Blastocystis hominis infection in a random sample of apparently healthy food handlers. A total of 415 individuals attending the Manoa Urban Outpatient Clinic (Caroní Municipality, Bolívar State, Venezuela) in the Adult Hygiene Program and who requested health certification to work as food handlers were studied. Stool samples obtained by spontaneous evacuation were examined by direct microscopy and the Willis concentration method. A total of 150 individuals were infected (36.14%), 107 (25.78%) of whom with B. hominis. There was no difference between males and females (p > 0.05), but there was a significant difference between ages (chi(2) = 12.17; g.l. = 4), with infection more frequent between 18 and 27 years. In 71.02% of the cases, B. hominis was the only parasite. Giardia lamblia was the parasite most frequently associated with B. hominis (2.41%). In the majority (85%) of infected individuals, less than five microorganisms per microscopic field were observed. We conclude that B. hominis is a frequent intestinal parasite among food handlers in Caroní Municipality, Bolivar State, Venezuela.

  19. In vitro effect of some Egyptian herbal extracts against Blastocystis hominis.

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    Abdel-Hafeez, Ekhlas H; Ahmad, Azza K; Andelgelil, Noha H; Abdellatif, Manal Z M; Kamal, Amany M; Mohamed, Rabie M

    2015-04-01

    Blastocystis hominis is an enteric parasite that inhabits the gastrointestinal tract of humans and many animals. This emerging parasite has a worldwide distribution. It is often identified as the most common eukaryotic organism reported in human fecal samples that showed a dramatic increase in recent years. Metronidazole is the main therapy for blastocystosis. However, frequent reports of treatment failure suggesting isolates resistance to metronidazole. This study determined the growth pattern and in vitro susceptibility of B. hominis to nitazoxanide (NTZ), garlic, ginger, onion and turmeric. Fecal samples positive for Blastocystis were collected from patients with irritable bowel syndrome (IBS), and processed for culture. Cultured samples were subjected to examination by light microscopy. Herbs' extracts was freshly prepared. Drug susceptibility assays was done using 0.1 mg/ml of NTZ, garlic, ginger, onion and turmeric. Effects assessed on parasite culture after 24 hr. and 48 hr. Cultured fecal samples of B. hominis have identified several forms of the organism; vacuolar, granular, amoeboid and cyst forms within 24 hr. Nitazoxanide treatment significantly (P onion, and turmeric treatments insignificantly lowered the number of the parasite after 48 hr. (P < 0.15 & < 0.22 respectively). PMID:26012223

  20. Investigation of the blastocystis hominis frequency in patients with irritable bowel syndrome

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    Bayram Pektaş

    2014-06-01

    Full Text Available AimIn this study, it was aimed to investigate the relationship between Blastocystis hominis infection and inflammatory bowel syndrome (IBS. Methods: In this study, the frequency of B. hominis in the stool samples of 52 patients applied to Microbiology laboratory and pre-diagnosed with irritable bowel syndrome in January 2013-June 2013 was investigated, retrospectively. Microscopic investigations were evaluated after macroscopic examination. For this purpose, the stool samples of the diarrheal cases were investigated by trichrome staining after they were prepared by native-lugol and formol ethyl acetate concentration method. The results were compared with the examination of 2160 stool samples sent to our laboratory during the same period. Results: Stool samples of 52 patients pre-diagnosed with IBS were accepted to our laboratory in January 2013-June 2013. 13 of the patients were found as B. hominis positive. Weight loss and anorexia was identified only in one patient while abdominal pain, diarrhea and gas complaints were identified in all of the IBH and B. hominis positive patients. During the same period, parasites were detected in 96 (4.4% of 2160 stool samples sent to our laboratory and the most common was B. hominis 48 (2.2%. 452 of these patients applied with diarrhea symptoms and B. hominis was detected in 36 samples (7.96%. Conclusion: The limited studies investigating the presence of B. hominis in patients with irritable bowel syndrome are far from illuminating the role of this agent in disease pathogenesis. We believe that further investigations should be performed. In this study, 25% of the patients were found as positive. J Clin Exp Invest 2014; 5 (2: 242-245

  1. Presencia de Blastocystis Hominis como agente causal de enfermedades gatrointestinales en la comuna 7 (Gaira del Distrito de Santa Marta

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    Sonja Liliana Lozano Socarras

    2013-10-01

    Full Text Available La Blastocystis hominis es un protozoo que causa cuadros diarreicos. Es altamente prevalente en poblaciones que no cuentan con servicios adecuados de higiene, alcantarillado y salud pública. La infección con Blastocystis hominis frecuentemente concomita con otros enteropatógenos de reconocida patogenicidad, además se ha reportado como parásito oportunista en pacientes con VIII SIDA. El objetivo del presente estudio es determinar la presencia de Blastocystis hominis en pacientes de consulta externa con síntomas asociados a enfermedades gastrointestinales, en la comuna 7 del distrito de Santa Marta, Colombia, durante el mes de Enero a Diciembre de 2004. El método de diagnóstico utilizado fue examen coproparasitológico seriado y el número de pacientes analiza-dos fue de 291. Los resultados muestran una alta presencia de Blastocystis hominken pacientes con enfermedad diarreica residentes en Gaira. Un alto porcentaje de la población parasitada (62,6% presento como único agente causal de la enfermedad diarreica al Blastocystis hominis lo que sugiere la presencia de otras enfermedades que pueden involucrar un compromiso inmunológico, el efecto será una respuesta inmune débil contra el parásito.

  2. Epidemiological survey of Giardia spp. and Blastocystis hominis in an Argentinian rural community.

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    Minvielle, Marta Cecilia; Pezzani, Betina Cecilia; Cordoba, María Alejandra; De Luca, María Marta; Apezteguia, María Carmen; Basualdo, Juan Angel

    2004-09-01

    The aim of this study was to relate personal data, socio-cultural and environmental characteristics, and the presence of symptoms/signs with the frequencies of Giardia spp. and Blastocystis hominis among a rural population in Buenos Aires Province, Argentina. Of the surveyed population (350), 3.7% were infected with only Giardia spp. or 22.9% with B. hominis, and 2.3% were infected with both protozoa. The frequency of infection according to sex; 6.1% of males were infected and 1.6% of females by Giardia spp., 26.7% and 19.5% by B. hominis, and 2.4% and 2.2% by both parasites, respectively. Giardia spp. was detected in only three adults (over 14 years), but B. hominis was more frequent in adults than in children. The prevalences of these protozoa in this community are lower than those reported by other Argentinean studies, which is probably associated with the low density of the studied population (5.95 inhab/km2). Statistical analysis revealed that a male sex, flooding of the home, the use of a latrine, and an abdominal pain were correlated with the presence of these parasites, which indicate the importance of these factors in rural communities. PMID:15381860

  3. Phenotypic and genotypic characterisation of Blastocystis hominis isolates implicates subtype 3 as a subtype with pathogenic potential.

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    Tan, T C; Suresh, K G; Smith, H V

    2008-12-01

    Despite frequent reports on the presence of Blastocystis hominis in human intestinal tract, its pathogenicity remains a matter of intense debate. These discrepancies may be due to the varying pathogenic potential or virulence of the isolates studied. The present study represents the first to investigate both phenotypic and genotypic characteristics of B. hominis obtained from symptomatic and asymptomatic individuals. Symptomatic isolates had a significantly greater size range and lower growth rate in Jones' medium than asymptomatic isolates. The parasite cells of symptomatic isolates exhibited rougher surface topography and greater binding affinity to Canavalia ensiformis (ConA) and Helix pomatia (HPA). The present study also identifies further phenotypic characteristics, which aided in differentiating the pathogenic forms from the non-pathogenic forms of B. hominis. Blastocystis subtype 3 was found to be correlated well with the disease. PMID:18795333

  4. Epidemiological survey of Blastocystis hominis in Huainan City, Anhui Province, China

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    Ke-Xia Wang; Chao-Pin Li; Jian Wang; Yu-Bao Cui

    2002-01-01

    AIM: To provide scientific evidence for prevention andcontrolling of blastocystosis, the infection of Blastocystishomonis and to study its clinical significance in Huainan City,Anhui Province, China.METHODS: Blastocystis homonis in fresh stools taken from100 infants, 100 pupils, 100 middle school students and403 patients with diarrhea was smeared and detected withmethod of iodine staining and hematoxylin staining. Afterpreliminary direct microscopy, the shape and size ofBlastocystis homonis were observed with high power lens.The cellular immune function of the patients withblastocystosis was detected with biotin-streptavidin (BSA).RESULTS: The positive rates of Blastocystis homonis inflesh stools taken from the infants, pupils, middle schoolstudents and the patients with diarrhea, were 1.0 % (1/100),1.0 % (1/100), 0 % (0/100) and 5.96 % (24/403)respectively. Furthermore, the positive rates of Blastocystishomonis in the stool samples taken from the patients withmild diarrhea, intermediate diarrhea, severe diarrhea andobstinate diarrhea were 6.03 % (14/232), 2.25 % (2/89),0 % (0/17) and 12.31% (8/65) respectively. The positiverates of Blastocystis homonis in fresh stools of male andfemale patients with diarrhea were 7.52 % (17/226) and3.95 % (7/177) respectively, and those of patients in urbanand rural areas were 4.56 % (11/241) and 8.02 % (13/162)respectively. There was no significant difference betweenthem (P>0.05). The positive rates of CD3+, CD4+, CD8+ inserum of Blastocystis homonis-positive and-negativeindividuals were 0.64±0.06, 0.44±0.06, 0.28±0.04 and0.60±0.05, 0.40±0.05 and 0.30±0.05 respectively, andthe ratio of CD4+/CD8+ of the two groups were 1.53±0.34and 1.27±0.22. There was significant difference betweenthe two groups (P<0.05, P<0.01).CONCLUSION: The prevalence of Blastocystis hominis asan enteric pathogen in human seems not to be associatedwith gender and living environment, and that Blastocystishominis is more common in stool samples of

  5. Complete circular DNA in the mitochondria-like organelles of Blastocystis hominis.

    Science.gov (United States)

    Wawrzyniak, Ivan; Roussel, Michaël; Diogon, Marie; Couloux, Arnaud; Texier, Catherine; Tan, Kevin S W; Vivarès, Christian P; Delbac, Frédéric; Wincker, Patrick; El Alaoui, Hicham

    2008-10-01

    Blastocystis hominis is an anaerobic parasite of the human intestinal tract belonging to the Stramenopile group. Using genome sequencing project data, we describe here the complete sequence of a 29,270-bp circular DNA molecule that presents mitochondrial features (such as oxidative phosphorylation complex I subunits) but lacks complexes III, IV and V. Transmission electron microscopy analyses reveal that this molecule, as well as mitochondrial (NADH:ubiquinone oxidoreductase subunit 7 (NAD7), beta-succinyl-CoA synthetase (beta-SCS)) and hydrogenosomal (pyruvate ferredoxin oxido-reductase (PFOR), iron-hydrogenase) proteins, are located within double-membrane surrounded-compartments known as mitochondria-like organelles (MLOs). As there is no evidence for hydrogen production by this organism, we suggest that MLOs are more likely anaerobic mitochondria. PMID:18694756

  6. Frequency of Blastocystis hominis and other intestinal parasites in stool samples examined at the Parasitology Laboratory of the School of Pharmaceutical Sciences at the São Paulo State University, Araraquara Freqüência de Blastocystis hominis e outros enteroparasitas em amostras de fezes examinadas no Laboratório de Parasitologia da Faculdade de Ciências Farmacêuticas da Universidade Estadual Paulista, Araraquara

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    Júlio César Miné

    2008-12-01

    como comparar diferentes métodos para a pesquisa desse protozoário em amostras de fezes. Foram estudadas 503 amostras de fezes submetidas ao exame direto a fresco, às técnicas de Faust e cols, Lutz e de Rugai e cols, além das colorações pela hematoxilina férrica, tricrômio e de Kinyoun modificada. Entre as 503 amostras examinadas, 174 (34,6% apresentaram-se positivas para a presença de parasitas intestinais. O protozoário e o helminto mais freqüentes foram Entamoeba coli (14,6% e Strongyloides stercoralis (6,7%, respectivamente. Blastocystis hominis foi observado em 23 (4,6% amostras fecais com consistência predominantemente pastosa, não caracterizando quadro diarréico. Apesar da baixa freqüência de Blastocystis hominis encontrada na região de Araraquara, comparativamente a outras regiões brasileiras, é importante a realização do diagnóstico laboratorial desse protozoário. O encontro de Blastocystis hominis em material fecal é indicativo de contaminação de alimentos e água de consumo, desde que se admita a rota de transmissão oral-fecal desse parasita, o que implica na orientação da população sobre as medidas de saneamento básico e higiene como meio para se controlar problemas de saúde ocasionados pelos enteroparasitas.

  7. Prevalence and Risk Factors of Giardia lamblia and Blastocystis hominis Infections in Children Under Ten Years Old, Hamadan, Iran

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    Sedighi

    2015-05-01

    Full Text Available Background Nowadays, parasitic infections are a major health problem throughout the world, particularly in the developing countries. Objectives Considering the high susceptibility of children against parasitic infections, the current study aimed to determine the prevalence and associated risk factors of intestinal parasitic infections among children less than 10 years old in urban and rural areas of Hamadan district. Patients and Methods The current study was conducted on 395 children (214 males and 181 females, referred to urban and rural health centers in Hamadan district in 2013. Stool samples were examined by formalin-ether concentration technique, and trichrome and modified Ziehl-Neelsen staining methods. The results were analyzed by chi-square test. Results Of the 395 studied children, 112 (28.4% were infected with intestinal parasites. Blastocystis hominis was the most frequently detected parasite with the prevalence of 18.5%, followed by Giardia lamblia (10.9%, Entamoeba coli (2.8%, Dientamoeba fragilis (0.8%, Iodamoeba buetschlii (0.8%, Chilomastix mesnili (0.5%, Cryptosporidium spp. (0.5%, Endolimax nana (0.3% and Entamoeba hartmanni (0.3%. No cases of infection with helminth parasites were found. Conclusions The results of the study showed a high prevalence of Giardia lamblia and Blastocystis hominis in rural areas compared to urban regions. Therefore it is necessary to promote the public health awareness in the rural population, in order to reduce the frequency of parasitic infections.

  8. Blastocystis hominis: occurrence in children and staff members of municipal day-care centers from Botucatu, São Paulo State, Brazil

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    Semíramis Guimarães

    1993-09-01

    Full Text Available To study the frequency of Blastocystis hominis among healthy individuals, feces were collected from 153 children and 20 staff members of some municipal day-care centers. Three separate stool specimens of each individual were processed by Lutz and Faust methods. From 173 studied individuals, 60 (34.7% showed B. hominis, frequently in association with other intestinal parasites and/or commensals. B. hominis was found mainly in adults and children between 36 and 72 months old. All positive cases were detected only by Lutz method and the use of three stool specimens increased the positivity of the parasitological diagnostic.

  9. The effects of extracts from anti-diarrheic Thai medicinal plants on the in vitro growth of the intestinal protozoa parasite: Blastocystis hominis.

    Science.gov (United States)

    Sawangjaroen, Nongyao; Sawangjaroen, Kitja

    2005-04-01

    The activities of n-hexane, dichloromethane and methanol extracts from five anti-diarrheic Thai medicinal plants, Acacia catechu (Fabaceae) resin, Amaranthus spinosus (Amaranthaceae) whole plant, Brucea javanica (Simaroubaceae) seed, Piper longum (Piperaceae) fruit and Quercus infectoria (Fagaceae) nut gall were tested against the in vitro growth of fresh isolates of the intestinal protozoan parasite, Blastocystis hominis. The extracts at concentrations that ranged from 62.5 to 2000 microg/mL, were incubated with several isolates of Blastocystis hominis for 48 h. The activities were classified as killed, inhibited, moderately inhibited and not-inhibited. Dichloromethane and methanol extracts from the Brucea javanica seed and a methanol extract from Quercus infectoria nut gall showed the highest activity. At a concentration of 2000 microg/mL, the three extracts killed 82, 75 and 67% of the Blastocystis hominis samples tested and inhibited 94, 100 and 76% of them, respectively. Metronidazole, used as a reference antiprotozoan drug, at a concentration of 40 microg/mL, killed 97% of the Blastocystis hominis isolates and inhibited all samples tested at concentrations that ranged from 1.25 to 20 microg/mL. PMID:15763365

  10. Prevalencia de Blastocystis hominis en vendedores ambulantes de comida del municipio Caroní, Estado Bolívar, Venezuela Prevalence of Blastocystis hominis among food handlers from Caroni municipality, Bolivar State, Venezuela

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    Ixora Requena

    2003-12-01

    Full Text Available Para determinar la prevalencia de Blastocystis hominis en una muestra de vendedores ambulantes de comida, aparentemente sanos, se realizó un estudio seccional con 415 personas que acudieron al Ambulatorio Urbano tipo III "Manoa" (Municipio Caroní, Estado Bolívar, Venezuela, Programa de Higiene del Adulto, a solicitar el certificado de salud para trabajar como vendedores de comida. Una muestra de heces obtenida por evacuación espontánea fue analizada mediante la técnica de examen directo y método de concentración de Willis. Se encontraron 150 personas parasitadas (36,14%, de ellas 107 (25,78% con B. hominis. No se observó predilección por el sexo (p > 0,05, pero sí con relación a la edad, siendo las personas de 18 a 27 años las más afectados (ji² = 12,17; g.l. = 4. En el 71,02% de los casos se encontró como parásito único y en 28,98% de los casos asociados a otros parásitos, siendo el más frecuentemente asociado Giardia lamblia (2,41%. En la mayoría de las personas parasitadas (85% el protozoario se observó en un número menor de cinco células por campo. Se concluye que B. hominis es un parásito frecuente en manipuladores de alimentos del Municipio Caroní, Estado Bolívar, Venezuela.A cross-sectional survey was conducted to determine the prevalence of Blastocystis hominis infection in a random sample of apparently healthy food handlers. A total of 415 individuals attending the Manoa Urban Outpatient Clinic (Caroní Municipality, Bolívar State, Venezuela in the Adult Hygiene Program and who requested health certification to work as food handlers were studied. Stool samples obtained by spontaneous evacuation were examined by direct microscopy and the Willis concentration method. A total of 150 individuals were infected (36.14%, 107 (25.78% of whom with B. hominis. There was no difference between males and females (p > 0.05, but there was a significant difference between ages (chi² = 12.17; g.l. = 4, with infection more

  11. [Protozoan infection (Blastocystis hominis) concomitant with Pseudomonas sp. peritonitis in continuous ambulatory peritoneal dialysis (CAPD)].

    Science.gov (United States)

    Boccardo, G; De Prisco, O; Ettari, G; Donato, G; Maurino, D; Savoia, D

    1996-03-01

    Case-report of protozoal infection (Blastocystis bominis) during Pseudomonas peritonitis in male patient with intestinal diverticulosis on continuous ambulatory peritoneal dialysis (CAPD) treatment for chronic renal failure (CRF). Microscopic morphology and cultural characteristics are summarized from current literature. Photographic images in phase contrast from fresh-observation of faeces and peritoneal fluid are reported. Although other Protozoa (e.g. Acanthamoeba free-living) have already been found in dialysis fluid, this is the first case, referred in literature, of Blastocystis bominis infection in CAPD patients. Some pathogenetic hypothesis are done involving Blastocystis bominis in peritoneal infection, especially in immunodepressed patients like dialysed ones. Although many chemotherapeutics are provided for this protozoiasis during enteritis, in our case no supplement was required except specific antibiotic therapy for Pseudomonas infection. Symbion or pathogen? Is now-a-day the question which troubles parasitologists. Systemic research of Protozoa in dialysed patients is anyhow advisable. PMID:8848771

  12. Prevalencia de Blastocystis hominis en vendedores ambulantes de comida del municipio Caroní, Estado Bolívar, Venezuela

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    Requena Ixora

    2003-01-01

    Full Text Available Para determinar la prevalencia de Blastocystis hominis en una muestra de vendedores ambulantes de comida, aparentemente sanos, se realizó un estudio seccional con 415 personas que acudieron al Ambulatorio Urbano tipo III "Manoa" (Municipio Caroní, Estado Bolívar, Venezuela, Programa de Higiene del Adulto, a solicitar el certificado de salud para trabajar como vendedores de comida. Una muestra de heces obtenida por evacuación espontánea fue analizada mediante la técnica de examen directo y método de concentración de Willis. Se encontraron 150 personas parasitadas (36,14%, de ellas 107 (25,78% con B. hominis. No se observó predilección por el sexo (p > 0,05, pero sí con relación a la edad, siendo las personas de 18 a 27 años las más afectados (ji² = 12,17; g.l. = 4. En el 71,02% de los casos se encontró como parásito único y en 28,98% de los casos asociados a otros parásitos, siendo el más frecuentemente asociado Giardia lamblia (2,41%. En la mayoría de las personas parasitadas (85% el protozoario se observó en un número menor de cinco células por campo. Se concluye que B. hominis es un parásito frecuente en manipuladores de alimentos del Municipio Caroní, Estado Bolívar, Venezuela.

  13. Prevalencia de Blastocystis hominis en vendedores ambulantes de comida del municipio Caroní, Estado Bolívar, Venezuela

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    Ixora Requena

    2003-12-01

    Full Text Available Para determinar la prevalencia de Blastocystis hominis en una muestra de vendedores ambulantes de comida, aparentemente sanos, se realizó un estudio seccional con 415 personas que acudieron al Ambulatorio Urbano tipo III "Manoa" (Municipio Caroní, Estado Bolívar, Venezuela, Programa de Higiene del Adulto, a solicitar el certificado de salud para trabajar como vendedores de comida. Una muestra de heces obtenida por evacuación espontánea fue analizada mediante la técnica de examen directo y método de concentración de Willis. Se encontraron 150 personas parasitadas (36,14%, de ellas 107 (25,78% con B. hominis. No se observó predilección por el sexo (p > 0,05, pero sí con relación a la edad, siendo las personas de 18 a 27 años las más afectados (ji² = 12,17; g.l. = 4. En el 71,02% de los casos se encontró como parásito único y en 28,98% de los casos asociados a otros parásitos, siendo el más frecuentemente asociado Giardia lamblia (2,41%. En la mayoría de las personas parasitadas (85% el protozoario se observó en un número menor de cinco células por campo. Se concluye que B. hominis es un parásito frecuente en manipuladores de alimentos del Municipio Caroní, Estado Bolívar, Venezuela.

  14. [Blastocystis hominis in Canavese: a retrospective study of samples received for fecal parasitological examination at the Ivrea- Castellamonte Hospital over 42 months].

    Science.gov (United States)

    Pistono, P G; Dusi, M P; Ronchetto, F; Cestonaro, G; Guasco, C

    1991-01-01

    In the last ten years the interest in the controversial Blastocystis hominis microorganism of the intestine has greatly increased. The authors conducted a retrospective study of feces for parasitology investigations at the Ivrea-Castellamonte hospital. These investigations lasted 42 months (1/1/1988-31/6/1991) and the authors focused their attention on samples with 5 or more Blastocystis hominis at the microscopically examination with a 40X phase objective. During the study 2,138 samples of feces (belonging to 1552 patients) arrived. 284 samples (13.3%), corresponding to 200 patients (12.9%), proved to be positive for one or more parasites. In 155 samples (7.2%) regarding 109 patients (7.0%)--53 males, 56 females--Blastocystis was present. These patients were between 2 and 78 years of age. In 19 (17.4%) of these patients other parasites were associated, parasites such as Giardia lamblia (in 6 cases), Entamoeba coli (in 4 cases), Entamoeba spp. (in 2 cases), Entamoeba histolytica (in one case), Entamoeba hartmanni (in one case), Iodamoeba butschlii (in one case), Hymenolepis nana (in one case); helminths (in 3 cases). The most effect proved to be those patients between 21 and 50 years of age (53 of 98 patients). Few infants were affected. The greatest incidence of Blastocystis hominis infection occurred during the months of January to June. The authors conclude by considering how the incidence data of such parasitosis in the Canavese region can be superimposed on as much as has been observed in territories which are very different regarding social-economic and geoclimatic conditions. The authors think it is necessary to start a prospective clinical-epidemiological investigation in the Canavese region with a view to defining the clinical approach to the parasite under discussion in a better way. PMID:1844921

  15. Studies on the vitro culture of Blastocystis hominis%人芽囊原虫的体外培养

    Institute of Scientific and Technical Information of China (English)

    乔继英; 张旭; 董晓慧; 李小琪; 杨珺华; 姚繁荣

    2001-01-01

    目的建立人芽囊原虫(Blastocystis hominis,B.h)的连续培养方法,并研究影响其生长的因素,为进一步研究其生活史、致病机理奠定基础.方法比较不同血清种类、血清量、温度、pH值等条件下虫体生长繁殖情况.结果B.h在Locke-egg-serum(LES)培养基中生长良好,其最适培养条件为:LES培养基中加含20%小牛血清的Locke液,青霉素、链霉素和两性霉素B,37℃培养,pH6.8~7.0,第5、9天虫体繁殖达高峰;pH7.2~7.8时,第3、6、9天虫体繁殖达高峰.结论B.h在LES培养基中生长良好,在合适的温度、血清、pH值等条件下,第3、6天或第5天为转种时间,可达到长期培养的目的.

  16. Clinical analysis of blastocystis hominis infection in children%小儿人芽囊原虫感染53例临床分析

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    胡缨; 陈国力; 李艳文

    2012-01-01

    目的:探讨小儿人芽囊原虫感染的临床特点及诊治方法.方法:对我院儿科收治的53例确诊为人芽囊原虫感染的临床资料进行分析.结果:47例患儿有消化道症状:腹泻31例,腹痛27例.患儿粪便中均查见人芽囊原虫,以空泡型最多见.实验室辅助检查中,66.04%患儿血红蛋白降低,3.77%患儿嗜酸性粒细胞升高.81.13%患儿经甲硝唑1个疗程治愈,其余2个疗程治愈,且3个月后复查均无复发.结论:对有消化道症状而病因不明的患儿须高度警惕人芽囊原虫感染,酌情配合使用直接涂片法和碘液染色法是确诊简单有效的途径.甲硝唑治疗效果良好.%Objective To Investigate the clinical characteristics and diagnostic methods of Blastocystis Hominis infection in children. Methods 53 children with Blastocystis Hominis infection admitted in the Pediatric Department were retrospectively analyzed. Results Alimentary disorders were found in 47 children, including diarrhea in 31 cases and abdominal pain in 27 cases. Blastocystis Hominis was found in all stool specimens with vacuolus type which is the most common. hemoglobin was decreased in 66.04% of patients, eosinophilic granulocytes was increased in 3.77%. So did the left 18.87% of patients after two courses. No recurrence was found 3 months later. Conclusion Blastocystis Hominis infection should be highly suspected in children with alimentary disorders but without obvious causes. Direct smear and Iodine staining are the simple and effective methods. Metronidazole can achieve good effect.

  17. The use of the antifungal agent miconazole as an inhibitor of Blastocystis hominis growth in Entamoeba histolytica/E. dispar cultures Uso do antifúngico miconazol como inibidor do crescimento de Blastocystis hominis em culturas de Entamoeba histolytica/E. dispar

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    Alessandra Queiroga Gonçalves

    2007-06-01

    Full Text Available In regions with high prevalence, Blastocystis hominis is frequently found in association with Entamoeba histolytica/E. dispar in xenic cultures. Its exacerbated growth is often superimposed on the growth of amebas, thus impeding the continuation of the amebas in the culture, within a few generations. The present study reports on the excellent efficacy (100% of the antifungal agent miconazole in eliminating B. hominis from cultures of E. histolytica/E. dispar, thereby maintaining the integrity of the trophozoites of the amebas. Nystatin presented low efficacy (33.3%.Em regiões de alta prevalência, Blastocystis hominis é freqüentemente encontrado em associação com Entamoeba histolytica/E. dispar em cultivos xênicos. Seu crescimento exacerbado se sobrepõe muitas vezes ao das amebas, impedindo a manutenção destas em cultura, dentro de poucas gerações. O presente estudo relata a excelente eficácia (100% do antifúngico miconazol na eliminação de B. hominis dos cultivos de E. histolytica/E. dispar, mantendo-se a integridade dos trofozoítos das amebas. A nistatina apresentou eficácia baixa (33,3%.

  18. Experimental Infection of Mice with Blastocystis hominis%人芽囊原虫感染小鼠试验

    Institute of Scientific and Technical Information of China (English)

    姚繁荣; 乔继英; 赵晏; 张旭; 杨珺华; 李小琪

    2005-01-01

    目的通过感染不同免疫状态ICR小鼠寻求人芽囊原虫(Blastocystis hominis,B.h)对小鼠的易感途径及有效感染数量.方法①将104、105、106个培养3代的B.h分别经口及直肠感染ICR小鼠.②106个B.h经直肠感染免疫功能低下该种小鼠,观察感染后不同时间小鼠胃肠道B.h繁殖情况及消化道组织病理改变.结果B.h经口及直肠两种途径均可使小鼠感染,免疫功能低下小鼠感染后出现行动迟缓、精神萎靡、嗜睡、体重下降等,部分小鼠出现腹泻,排粘液便等症状,个别小鼠死亡.经解剖肉眼观察见空回肠、回盲部、结肠的肠壁组织严重水肿、充血、淤血等.在小鼠胃肠道内容物中均发现B.h.病理切片显示,小肠及结肠黏膜上皮脱落,黏膜下层不同程度的炎性细胞浸润,腺体结构不完整.结论B.h经直肠比经口更易感染小鼠,B.h可寄生于小鼠整个胃肠道.小鼠免疫功能降低时,B.h可迅速繁殖、致病性增强,并引起严重的肠黏膜病理改变.

  19. Observation on growth status of Blastocystis hominis in three media%3种培养基中芽囊原虫生长状况观察

    Institute of Scientific and Technical Information of China (English)

    俞英昉; 吴秀萍; 储言红; 陈家旭; 田利光

    2016-01-01

    Objective To observe the growth situation of Blastocystis hominis in vitro and select the optimal method for culti⁃vation of B. hominis in different media. Methods Ten positive stools with B. hominis were inoculated in three different media for cultivating,namely 1640,Jone’s medium and vitro medium. And the stools with good growth status and high quantities of B. hominis were chosen to inoculate in the three media with equal amount after subcultivation,and the number of B. hominis was counted every 24 h for ten days,and the morphological changes and growth status were also observed. Results The densities of B. hominis in the 1640 and Jone’s medium were higher than that in the vitro medium 48 h after the inoculation. The same stool sample was inoculated to the three different media and observed for ten days,and the results indicated that the growth of B. homi⁃nis presented regular changes in the three media,the growth peaks were on the third,sixth and ninth day post inoculation;and the density of B. hominis was the highest in the Jone’s medium. The morphology of B. hominis was the clearest and most dynamic in the vitro medium,while various reproductive forms were observed in the Jone’s medium. Conclusion Jone’s medium is suitable for the growth of B. hominis and can be the first choice for the cultivation of B. hominis in vitro,and vitro medium is the best medium for observing the growth situation of B. hominis.%目的:观察芽囊原虫在不同培养基中的生长状况,筛选合适的芽囊原虫培养方法。方法将10份芽囊原虫阳性粪便分别接种至Jone’s液、vitro液、16403种培养基中培养,选取生长情况良好、虫密度高的一份粪便传代培养后等量接种至3种不同的培养基中,每24 h计数虫体数量一次,连续观察10 d,并观察芽囊原虫的形态学变化和生长情况。结果10份粪便分别接种至3种培养基中培养,48 h后发现1640和Jone’s液中的虫密度高

  20. Blastocystis hominis infection in a post-cardiotomy patient on extracorporeal membrane oxygenation support: A case report and literature review

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    Chih-Hsuan Chen

    2014-01-01

    CONCLUSION: This is the first published article showing that the opportunistic pathogen, B. hominis, can cause severe infection in patients on ECMO support, a result that should be kept in mind when patients come from a place with a high prevalence of B. hominis. The prophylactic medication should be administered routinely when patients live in the region and extracorporeal life-support is used.

  1. 人芽囊原虫在199单相培养基中的体外培养%Culture of Blastocystis hominis in vitro in Medium 199

    Institute of Scientific and Technical Information of China (English)

    魏志超; 李亚清; 张旭; 乔继英

    2006-01-01

    目的 观察199单相培养基对人芽囊原虫(Blastocystis hominis)的体外培养情况.方法 采用199单相培养基对B.hominis进行体外连续培养,比较不同pH值、血清种类及接种量等条件下虫体生长繁殖情况及影响因素.结果 B.hominis 在199培养基中最适培养条件为:10%~20%小牛、人或马血清;接种量不小于1×105原虫/管;青、链霉素于37 ℃条件下厌氧培养;pH 为7.0、7.5及8.0时,B.hominis繁殖高峰分别为第4、8天,第3、6、9天和第2、4、7、9天.结论 使用199单相培养基可以对B.hominis进行体外长期培养.

  2. 西安地区人芽囊原虫感染状况调查%Investigation of Blastocystis hominis infection in Xi'an city, Shaanxi province

    Institute of Scientific and Technical Information of China (English)

    乔继英; 李小颖; 蔡娟; 张旭; 魏志超; 李亚青

    2011-01-01

    Objective To obtain the basic data of Blastocystis hotninis infection in Xi' an city, Shaanxi province. Methods Fecal samples of a total of 781 people including district residents, middle school students, animal keepers and diarrheal patients were examined for Blastocystis hominis in Nov.-Dec. 2007. Wet mount smear and iodine staining were employed to exam, and pictures were taken when necessary. Results Infection rates of Blastocystis hominis in healthy people and diarrheal patients were 1.99%( 11/554) and 7.05%( 16/227), respectively. Chi-square tests revealed that there was significant difference between them (x2=12.367,P0.05). The results in blood-containing mucous feces and other diarrhea samples (watery feces, unformed feces and fat diarrhea) were 12.16%(9/74) and 7%(7/153) , respectively, with significant difference (x2=4.382,.P<0.05). Furthermore, there was significant differences in different age groups (x2=18.632,P<0.05). Vacuolar, granular and cyst form cells, and budding, binary fission and schizogony were observed. Conclusion There were significant differences on the distributions of Blastocystiis hominis in different age groups, in status of health (healthy and diarrheal) and in patients with different kinds of diarrhea. The vacuolar and granular forms, and binary fission and budding were often observed during investigation.%目的 了解西安市人芽囊原虫的感染状况.方法 采集西安市社区居民、中学生、动物饲养人员、腹泻病人共781名受检者粪便标本,采用生理盐水直接涂片法及碘液染色法,典型形态使用数码显微镜摄像.结果 健康人群和腹泻人群人芽囊原虫的感染率分别为1.99%(11/554)和7.05%(16/227),差异具有统计学意义(x2=12.367,P<0.05);社区居民、中学生、动物饲养人员的感染率分别为2.20% (6/273)、1.20%(3/250)、6.45%(2/31),差异无统计学意义( x2=4.033,P>0.05);粘液脓血便人群的感染率为12.16%(9/74),其他腹

  3. 人芽囊原虫多样化繁殖方式短暂共存现象%Temporary coexistence phenomenon of diversified reproductive mode of Blastocystis hominis

    Institute of Scientific and Technical Information of China (English)

    张红卫; 贺丽君; 李文; 颜秋叶; 苏云普; 薛长贵; 王东

    2005-01-01

    目的为了解人芽囊原虫对外界条件的适应性,观察培养条件改变对其形态和繁殖方式的影响. 方法从腹泻患者粪便中检查并分离培养获得人芽囊原虫,分别用locke氏-鸡蛋双相培养基(LES)和改良1640培养基(RPMI-1640)培养,改变培养条件,观察人芽囊原虫形态和繁殖方式. 结果在两种培养基中均可见到人芽囊原虫的四种主要形态:空泡型、颗粒型、阿米巴型和包囊型,其繁殖方式以二分裂为主,可见少量出芽生殖.当从LES培养基突然变为RPMI-1640培养基时,人芽囊原虫出现短时间(48~72h)内生长停滞,随后出现多种形式的繁殖方式,可见较多的二分裂、出芽生殖、三分裂、多分裂、裂体生殖,持续24h后恢复为二分裂为主. 结论培养条件改变能够引起人芽囊原虫繁殖方式的改变,出现短暂性多样化繁殖方式,人芽囊原虫对环境的适应能力很强.%Objective To understand the adaptability of Blastocystis hominis (B. hominis ) to environment, observe effect of culture condition change on reproductive mode of B. hominis. Methods B. hominis was isolated from patient with diarrhea and cultured in different culture medium including Locke-egg-serum medium culture (LES) and modified RPMI-1640 culture medium (RPMI-1640). When culture condition was changed between LES and RPMI-1640, morphous and reproductive modes were observed. Results All of 4 form including vacuolar form, granular form, ameba form and cyst form were found in each culture medium, separately, and main reproductive mode is binary fission, a few of gemmation was exist. When culture condition was changed abruptly from LES medium to RPMI-1640 medium, different reproductive mode including binary fission, ternary fission,multiple fission and gemmation were found after growth retardation of 24 ~ 72 hours. This phenomenon continued less than 24 hours and binary fission remained reproductive mode then. Conclusion Reproductive

  4. Comparative study of frequency of Blastocystis hominis pre-schoolers from an urban area and rural area of Cartagena de Indias and its relation with signs and symptoms and risk factors

    Directory of Open Access Journals (Sweden)

    Mavianis Pinilla Pérez

    2015-08-01

    Full Text Available Background: the intestinal parasitism is common infections in the world and of higher prevalence in the poor community of developing countries. Until ending of decade of eighties, Blastocystis hominis is not reported in a routine way, being only a differential diagnostic problem; but in the nineties by means of an appropriate diagnosis increase its prevalence of 1.5 to 10 % in developed countries and with 30 to 50 % in developing countries. Objective: to compare frequency of Blastocystis hominis in preschoolers from an urban area and rural area of Cartagena de Indias and its relation with signs and symptoms and risk factors during the year 2013. Materials and methods: 286 samples of feces of children between 1 and 5 years old from an urban area (Crespo and rural area (La Boquilla small town were analyzed by serial coprological technique. Results: were found parasites in 38.5% (55/143 of children from urban area and 79% (113/143 of children from rural area. It was found association between presence of parasites with origin of children (X2= 48.53; p<0.0001. We reported a frequency of 51.1% de B. hominis in an urban area and 44.8 % in a rural area. Conclusions: the absence of monitoring and control of cases suggests the need to formulate and implement measurements guided to improve the quality of life in studied areas.

  5. Determination and Significance of Cytokines in Peripheral Blood of Patients Infected with Blastocystis Hominis%人芽囊原虫病患者外周血细胞因子测定及其意义

    Institute of Scientific and Technical Information of China (English)

    陶霖; 姜海行; 金群馨; 唐国都; 伍玲园; 卢作超

    2014-01-01

    目的:研究白细胞介素-8(IL-8)、白细胞介素-18(IL-18)、粒-巨噬细胞集落刺激因子(GM-CSF)在人芽囊原虫病中的作用。方法用酶联免疫吸附试验(ELISA)测定30例人芽囊原虫病患者外周血中IL-8、IL-18、GM-CSF水平,并与正常对照组进行比较。结果人芽囊原虫病患者外周血中IL-8、IL-18、GM-CSF水平显著高于正常对照组( P <0.05)。 IL-8、GM-CSF 水平随感染程度加重而升高( P <0.05)。结论 IL-8、IL-18、GM-CSF可能在人芽囊原虫感染发病中发挥作用。%Objective To investigate the role of interleukin-8 ( IL-8 ) , interleukin-18 ( IL-18 ) , granulocyte-macrophage colony-stimulating factor ( GM-CSF ) in Blastocystis hominis .Methods The levels of IL-8 , IL-18 , GM-CSF in peripheral blood of 30 cases of Blastocystis hominis were measured with enzyme-linked immunosorbent assay (ELISA).And the levels were compared with the normal controls .Results The levels of IL-8,IL-18,GM-CSF in peripheral blood of patients with Blastocystis homini were significantly higher than those of normal control group (P<0.05).The levels of IL-8,GM-CSF increased with increasing severity of infection (P <0.05).Conclusion IL-8 ,IL-18 ,GM-CSF may play an important role in the pathogenesis of Blastocystis hominis infection .

  6. 小儿人芽囊原虫病治疗72例临床观察%72 cases of clinical observation in pediatric blastocystis hominis disease treatment

    Institute of Scientific and Technical Information of China (English)

    朱经林; 刘南海; 黄要赣; 黄小青; 刘井秀

    2012-01-01

    目的:探讨甲硝唑联合阿奇霉素在小儿人芽囊原虫(Blastocystis hominis,B.h)感染治疗的临床意义.方法:对门诊小儿腹泻患者均采用便后立即检测,粪便生理盐水涂片碘染色后在显微镜下检查进行确诊,确诊后采用随机分组观察,甲硝唑治疗组24例、阿奇霉素治疗组24例和甲硝唑加阿奇霉素联合治疗组24例,观察3组疗效情况.结果:甲硝唑治疗组和阿奇霉素治疗组有效率分别为62.5%,66.6%,联合用药组为95.8%,甲硝唑治疗组和阿奇霉素治疗组统计学上无差异,P> 0.05;联用药组与甲硝唑组疗效有统计学差异,P<0.01;联合用药组与阿奇霉素组疗效有统计学差异,P<0.05;治疗6天与9天疗效无显著差异,P>0.05.结论:甲硝唑、阿奇霉素对人芽囊原虫感染均有较好治疗作用,联合用药比单用药疗效较好,疗程以6天为宜.

  7. 改良RPMI 1640培养人芽囊原虫的实验观察%Experimental observation of Blastocystis hominis cultured in Modified RPMI 1640

    Institute of Scientific and Technical Information of China (English)

    李小花; 邓婷; 李娟

    2013-01-01

    目的:探讨自制改良RPMI 1640培养基的效果.方法:收集lock氏液鸡蛋斜面双相培养基(LES)中的人芽囊原虫(Blastocystis hominis,B.h)滋养体,按2×105细胞每管接种于普通1640和改良1640培养基中,加入20%小牛血清及抗生素,调整pH值为7.5,除氧置37℃厌氧罐中培养.每24h计数一次,观察B.h在培养基中的虫体形态、生长高峰和增殖数量.结果:10天内改良1640培养基的pH值恒定为7.5;虫体直径4~8 μm,形态结构清晰,虫体规则,以空泡型为主,颗粒型可见,包囊较普通1640培养基中少;生长高峰期为第4~5天,生长周期均与普通1640培养基中的相似,但虫体达高峰值后下降较慢.结论:改良RPMI1640适宜于人芽囊原虫的体外增殖培养.

  8. Parasitoses intestinais de indígenas da comunidade Mapuera (Oriximiná, Estado do Pará, Brasil: elevada prevalência de Blastocystis hominis e encontro de Cryptosporidium sp e Cyclospora cayetanensis Intestinal parasitosis in Indians of the Mapuera community (Oriximiná, State of Pará, Brazil: high prevalence of Blastocystis hominis and finding of Cryptosporidium sp and Cyclospora cayetanensis

    Directory of Open Access Journals (Sweden)

    Jaila Dias Borges

    2009-06-01

    Full Text Available Foi avaliada a ocorrência de parasitoses intestinais em indígenas da aldeia Mapuera (Oriximiná, Estado do Pará, Brasil. No contexto de apreciações congêneres, expressa contribuição para adequado conhecimento do assunto, significativo sob o ponto de vista médico-sanitário. O exame parasitológico das fezes, de 83 pessoas, realizado por meio de quatro métodos, pode ser considerado como dotado de razoável amplitude para estabelecer diagnósticos. Ocorreu encontro de cistos de protozoários e de ovos de helmintos de múltiplos tipos, até mesmo em expressivas porcentagens, merecendo destaque a muito freqüente presença de Blastocystis hominis (57,8%, como também o encontro de Cryptosporidium sp (3,6% e de Cyclospora cayetanensis (10,8%, comentado especificamente. O verificado demonstra que tais índios vivem em ambiente onde prevalecem más condições higiênicas, em especial, facilitador da disseminação de protozoários e helmintos pelo contato com o solo ou ingestão de água e alimentos contaminados.Occurrences of intestinal parasitosis in Indians of the Mapuera community (Oriximiná, State of Pará, Brazil were evaluated. Within the context of group assessment, this study makes a contribution towards adequate knowledge of this subject, which is significant from a medical-sanitary point of view. Parasitological examination of feces from 83 individuals, performed using four different methods, could be considered to have reasonable amplitude for establishing diagnoses. Protozoan cysts and helminth eggs of many types were found, even with significant percentages. The frequent presence of Blastocystis hominis (57.8%, along with findings of Cryptosporidium sp (3.6% and Cyclospora cayetanensis (10.8%, deserved highlighting with specific comments. The findings show that these Indians live in an environment in which poor hygiene conditions prevail. In particular, these facilitate the dissemination of protozoa and helminths through

  9. 桂林市不同人群人芽囊原虫感染的流行病学调查分析%Epidemiological investigation on infection of blastocystis hominis in various populations in Guilin

    Institute of Scientific and Technical Information of China (English)

    农子军; 莫刚; 李云萍; 蒋莉萍; 石焕焕

    2012-01-01

    Objective To investigate the infection status of blastocystis hominis in various populations in Guilin city to provide basis for formulating intervention measures. Methods Self-designed questionnaire and stratified cluster sampling methods were a-dopted to collect the human fresh dejecta from 1603 persons, which were examined under a microscope after being dealt with improved centrifugal sedimentation with hydrochloric acid and aether. Results The detection rate of the blastocystis hominis among the people in Guilin was 10. 98% ; while the infection rate of the people aged 16 - 25 years was the highest(13. 35%) , male was higher than female(14. 58% and 8. 44% respectively) ,ethnic minorities higher than Han nationality,and suburban higher than ur-ban(14. 16% and 8. 69% respectively). The differences all had statistical significance(Pblastocystis hominis infections.%目的 了解桂林市不同人群中人芽囊原虫感染情况,为制定干预措施提供依据.方法 采用自行设计调查表、分层整群抽样调查的方法,收集1 603例不同人群当日新鲜粪便,用改良酸醚离心沉淀法处理后在显微镜下观察.结果 桂林市人芽囊原虫检出率为10.98%,人群感染率以15~<25岁组最高(13.35%),女性高于男性(分别为14.58%、8.44%),少数民族高于汉族,市郊高于市区(分别为14.16%、8.69%),经统计学处理,差异均有统计学意义(P<0.01).结论 加强初级卫生保健工作,针对不同人群特点开展卫生宣传教育,提高自我保健意识,以预防和控制人芽囊原虫感染的发生.

  10. A survey of Blastocystis in reptiles.

    Science.gov (United States)

    Teow, W L; Ng, G C; Chan, P P; Chan, Y C; Yap, E H; Zaman, V; Singh, M

    1992-01-01

    A total of 28 species of reptiles were investigated for Blastocystis using light microscopy and in vitro culture in biphasic egg slant medium. Blastocystis species were detected in 8 (28.6%) of these 28 species in 3 tortoises (Geochelone elephantopus, G. elegans and G. carbonaria), 3 snakes (Boiga dendrophilla, Python reticulatus and Elaphe radiata), 1 crocodile (Crocodylus porosus) and 1 iguana lizard (Cyclura cornuta). The reptilian Blastocystis appeared to be morphologically similar to B. hominis. PMID:1495927

  11. Survey on Blastocystis hominis infection in HIV positive individuals in Fuyang City, Anhui Province%安徽阜阳HIV阳性者合并人芽囊原虫感染及其影响因素分析

    Institute of Scientific and Technical Information of China (English)

    田利光; 王珍丽; 汪天平; 周晓农; 陈家旭; 程国金; 汪峰峰; 郭俭; 尹晓梅; 蔡玉春; 周莉; 童小妹

    2012-01-01

    Objective To understand the epidemiological characteristics of co-infection of HIV and Blastocystis hominis and its risk factors. Methods A total of 309 people with HIV positive in the development zone of Fuyang City were recruited, and the face to face questionnaires were conducted to collect the information of behavioral characteristics and sociodemographic data of the participants. Meanwhile, the samples of stool and blood were collected to test B. hominis, cytokines and CD4+/CD8+ T-lymphocyte. The influencing factors of co-infection of HIV and Blastocystis hominis were analyzed by the single factor analysis and Logistic regression analysis. Results Among the 309 people involved, 302 accepted feces examinations, 286 accepted the questionnaire investigation, and 263 accepted both of them. The infection rate of B. hominis was 17.11%, that of the female was 21.90%, which was significantly higher than that of the male (11.90%) (P < 0.05). The results from the multivariale Logistic regression model showed that good nutrition was significantly associated with the co-infection of HIV and B. hominis (OR = 0.263, 95% CI: 0.073, 0.945). Conclusions The infection rate of B. hominis is high in people with HIV positive, and the nutrition situation of individuals may be one of the important risk factors associated with co-infection.%目的 了解安徽省阜阳市HIV阳性者合并人芽囊原虫感染情况及其影响因素.方法 采用横断面调查的方法对安徽省阜阳市开发区309名HIV阳性者进行问卷调查,并采集其粪便和血液样本用于人芽囊原虫、CD4+T淋巴细胞和细胞因子检测.采用单因素分析和Logistic回归分析对影响HIV和人芽囊原虫合并感染的因素进行分析.结果 在309名参与调查的HIV阳性者中,302人参与粪样检测,286人参与问卷调查,同时参与2项检测者263人.调查发现,HIV阳性者合并人芽囊原虫感染率为17.11%,其中女性感染率为21.90%,明显高于男性(11

  12. INVESTIGATION OF COLLEGE STUDENT INFECTION WITH BLASTOCYSTIS HOMINIS IN GUANGXI MEDICAL UNIVERSITY%广西医科大学在校大学生人芽囊原虫感染情况调查

    Institute of Scientific and Technical Information of China (English)

    刘腾; 陈曦; 易乾彦; 邹齐; 刘晓泉; 石焕焕; 何姗姗; 王鸽; 符瑞佳; 林俊仪; 罗宝花; 韦祎

    2011-01-01

    目的:了解广西医科大学在校大学生人芽囊原虫(Blastocystis hominis,B.h)的感染情况.方法:应用酸醚离心沉淀法粪检,抽查3个年级共1 433人,统计分析民族、性别、生源地、不同年级组别之间B.h感染率.结果:广西医科大学在校大学生B.h平均感染率为14.93%;汉族感染率13.48%,壮族感染率18.38%,其他民族感染率为17.24%.各年级感染率最低为13.19%,最高达16.28%.男性感染率为14.74%;女性感染率为15.06%.广西本地生源感染率为15.06%;外地生源感染率为13.94%.结论:广西医科大学在校大学生B.h感染率较高,并且存在校内感染的可能性.壮族学生B.h感染率高于汉族.%Objective:To investigate the prevalence of college student infection with Blastocystis hominis in Guangxi Medical University. Methods: A random sample of 1 433 students from 3 grades was obtained. Testing for infection was done by means of modified formalin ether sedimentation and microscopic examination. Results:The infection rate was 14. 93% in total population, 13. 48% in Han, 18. 38% in Zhuang,and 17. 24% in other ethnic groups. The rate of infection for grade group was 13. 19% to 16. 28%. Male infection rate was 14.74% while the female was 15.06%. The infectious rate was 15.06% in studeuts from Guangxi, 13. 94% in students from other provinces. Conclusion:The rate of B. Hominis infection in Guangxi Medical University is high. There is the possibility of campus infection. And the infection of Zhuang is higher than Han.

  13. 广西两所三甲医院住院患者人芽囊原虫感染调查%INVESTIGATING BLASTOCYSTIS HOMINIS INFECTION OF INPATIENTS IN TWO TERTIARY HOSPITALS OF GUANGXI

    Institute of Scientific and Technical Information of China (English)

    王贝贝; 燕慧; 石焕焕; 刘登宇; 廖德君; 刘晓泉; 卢作超

    2016-01-01

    目的:了解广西两所三甲医院住院患者人芽囊原虫(Blastocystis hominis,B.hominis)的感染情况.方法:收取广西两所三级甲等医院(广西医科大学第一附属医院、附属肿瘤医院)住院患者新鲜粪便,采用改良酸醚沉淀法,镜检定性诊断,用R2.11.1分析住院患者职业及受教育水平各组间B.hominis感染率.结果:两所医院共检查住院患者2800例,总阳性率为36.6%.一附院与肿瘤医院阳性率分别为22.59%、43.24%.与正常人群感染率相比,住院患者感染率高于正常人群(P<0.001);肿瘤患者与非肿瘤患者、正常人群感染率相比,差异有统计学意义(P<0.001);按照消化道疾病患者与非消化道疾病患者分组,两组感染率差异有统计学意义(P<0.001);按照文化程度、职业划分,两所医院初中及以下学历患者、农民患者感染率较其他患者感染率高且差异有统计学意义(均P<0.001).结论:住院患者较正常人群易于感染B.hominis,而其中肿瘤患者和消化道疾病患者则更易于感染B.hominis.

  14. 广西10例人芽囊原虫基因型和同工酶谱的研究%Genotype Analysis and Isoenzyme Patterns of Ten Isolates of Blastocystis hominis from Guangxi

    Institute of Scientific and Technical Information of China (English)

    战廷正; 石焕焕; 何姗姗; 刘腾

    2012-01-01

    Objective To analyze genotypes and lactate dehydrogenase (LDH) and esterase (EST) patterns in 10 isolates of Blastocystis hominis collected from Guangxi. Methods Ten B. Hominis isolates (B/iGXl~B/iGX10) were obtained from the fecal specimens of patients and cultivated in vitro, and then the genomic DNA was extracted. The isolates were genotyped by PCR using seven pairs of known sequenced-tagged site (STS) primers. Isoenzyme patterns of LDH and EST were investigated by SDS-PAGE. Results Out of the 10 isolates, 8 were identified as genotype I and the genotypes of the other two (BAGX4 and BhGXl) were unknown which were negative to all the STS primers. Among the ten isolates, 10 LDH bands were found, more with Rm37, Rm49, Rm57, Rm68 and Rm92. 12 bands showed in EST patterns: Rml4, Rml8, Rm23, Rm27, Rm35, Rm41, Rm45, Rm50, Rm55, Rm68, Rm77 and Rm82. Difference existed with the LDH and EST patterns among the isolates. Conclusion Genotype I is the major one in the 10 B. Hominis isolates from Guangxi, and the isolates show different isoenzyme patterns for LDH and EST.%目的 分析广西10个人芽囊原虫(Blastocystis hominis)分离株基因型,及其乳酸脱氢酶(LDH)和酯酶(EST)的同工酶谱特征.方法 从感染者粪便中分离到的10个人芽囊原虫(BhGX1~BhGX 10),体外培养并提取基因组DNA.用已知的7对特异性序列标记位点(sequence tagged sites,STS)引物PCR扩增,来鉴定基因型.采用十二烷基硫酸钠-聚丙烯酰胺凝胶电泳(SDS-PAGE),分别进行乳酸脱氢酶和酯酶2种同工酶染色,比较分析10个分离株的酶谱. 结果 10个人芽囊原虫分离株中有8个为基因Ⅰ型;另外2个分离株(BhGX4和BhGX7)经7对引物扩增均为阴性,为未知基因型.10个人芽囊原虫分离株在LDH谱中共出现10条酶带,常见酶带为Rm37、Rm49、Rm57、Rm68和Rm92;在EST谱中共出现12条酶带,其中Rm14、Rm18、Rm23、Rm27、Rm45、Rm50和Rm77酶带较常见.各分离株之间的LDH和EST同工

  15. 人芽囊原虫在RPMI1640培养基中培养条件的优化%The Optimization of in Vitro Culture Conditions of Blastocystis hominis in RPMI 1640 Mediums

    Institute of Scientific and Technical Information of China (English)

    答嵘; 乔继英; 王伟; 成少利; 卢朝辉; 张旭

    2006-01-01

    目的优化人芽囊原虫(Blastocystis hominis)在RPMI1640培养基中的培养条件.方法将B.hominis接种至RPMI1640培养基中,观察酸碱度、接种量、血清种类、浓度、温度、空气中氧与粪便标本的选择对B.hominis生长的影响.结果在RPMI1640培养基中,pH7.5、接种量200 000细胞/管、20%小牛血清,37℃,加入青霉素、链霉素为最佳培养条件.结论在RPMI1640中,接种以B.hominis阳性(颗粒型为主)的血粘液便,加入20%小牛血清,青霉素、链霉素,在pH7.5于37℃条件下厌氧培养,每6 d转种一次,可达到长期培养B.hominis的目的.

  16. 腹泻患者及培养基中人芽囊原虫的各期形态研究%Study on Morphology of Blastocystis hominis in Culture and from Diarrhea Patients

    Institute of Scientific and Technical Information of China (English)

    张旭; 乔继英; 董小惠; 李亚青; 李小琪; 李琛

    2003-01-01

    目的研究光镜下各期人芽囊原虫(Blastocystis hominis,B.h)形态,以进一步研究其生活史及致病性,并为临床检验提供形态依据.方法使用洛克氏液-鸡蛋-血清培养基,对腹泻患者粪便的B.h进行体外连续培养,经碘液染色及铁苏木素染色,在光镜下研究其各期形态、结构.结果通过形态学研究观察到空泡型、颗粒型、阿米巴型及包囊型以及各型之间的相互转化.结论B.h形态多样,临床多见空泡型和颗粒型.空泡型可成囊.

  17. 儿童反复腹痛人芽囊原虫的检测和治疗%Examination and Treatment of the Blastocystis Hominis Infection in the Child Repeatedly Abdominal Pain

    Institute of Scientific and Technical Information of China (English)

    廖红群; 邱伟; 王玲; 刘小生; 苏水莲

    2010-01-01

    目的:探讨儿童反复腹痛与人芽囊原虫(Blastocystis hominis B.h)感染的关系和相应的治疗.方法:将200例反复腹痛患儿进行人芽囊原虫检测并进行相应的治疗.结果:发现反复腹痛患儿人芽囊原虫感染人数66例,感染率为33%,正常儿童人芽囊原虫感染人数9例,感染率为7.5%,两者差异有统计学意义.通过相应的治疗,总有效率为90.9%.结论:儿童反复腹痛患儿的人芽囊原虫感染率很高,该研究拓宽了反复腹痛儿童病因的检测范围,使治疗更有针对性.

  18. Blastocystis hominis infection in children with diarrhea and abdominal pain: clinical analysis of 28 cases%儿童腹痛、腹泻人群中人芽囊原虫的检测:附28例分析

    Institute of Scientific and Technical Information of China (English)

    王玲; 苏水莲; 陈桂风

    2006-01-01

    人芽囊原虫(Blastocystis hominisB.h)是一种广泛寄生于人和许多动物肠道的原虫,曾长期被误认为是一种对人体无害的“肠道酵母菌”。近年研究表明,它是一种寄生在肠道的可致病的原虫,主要引起人体胃肠功能紊乱。本研究从2004年10月~2006年1月对本市0~14岁临床具有反复腹痛、腹泻的1164例患儿,应用Lock’s鸡蛋斜面血清培养基进行大便培养,经病原学确认,其中已感染B.h者419例,感染率达35.9%。现将419例中临床资料较完整的28例分析如下。

  19. Observation of morphologic changes of Blastocystis hominis in digestive tract of infected mice%消化道人芽囊原虫感染小鼠形态观察

    Institute of Scientific and Technical Information of China (English)

    姚繁荣; 乔继英; 张旭; 杨珺华; 李小琪

    2006-01-01

    通过观察感染小鼠消化道中人芽囊原虫(Blastocystis hominis, B.h)的生长繁殖状况及形态特点,为进一步研究B.h的生理学特性及致病机制提供依据.用含106个B.h的虫体液经直肠感染免疫功能低下ICR小鼠,消化道内容物苏木素染色后光镜下观察和透射电镜观察.在感染小鼠消化道内容物中发现了与粪便及培养物不同的B.h形态.结果表明,提示体内外不同的生长环境对B.h的形态变化产生较大的影响,这种不同的形态可能与其致病性密切相关.

  20. Vitro culture of Blastocystis hominis in medium DMEM%人芽囊原虫在DMEM单相培养基中体外培养方法的建立

    Institute of Scientific and Technical Information of China (English)

    张旭; 乔继英; 答嵘; 李亚青; 姚繁荣

    2006-01-01

    目的 建立人芽囊原虫(Blastocystis hominis,B.h)在DMEM单相培养基中的体外连续培养方法,为进一步研究其诊断、生活史、致病机制奠定基础.方法 比较不同pH值、血清种类、血清浓度及接种量等条件下虫体生长繁殖情况及影响因素.结果 使用DMEM培养基、接种量大于105/管、pH7.0~8.0、10%~30%小牛血清(或人、马血清),青、链霉素及二性霉素B,于37 ℃条件下厌氧培养,每三、六或五天转种一次,可达到体外长期培养B.h的目的.结论 使用DMEM单相培养基可用于人芽囊原虫的诊断及体外长期培养.

  1. 人芽囊原虫在IMDM单相培养基中的体外培养效果观察%Culture of Blastocystis hominis with IMDM medium in vitro

    Institute of Scientific and Technical Information of China (English)

    唐莉莉; 卢作超; 战廷正; 符瑞佳; 伍玲园; 刘登宇

    2010-01-01

    目的 观察IMDM(Iscove's Modified Dulbecco's Medium)单相培养基对人芽囊原虫(Blastocystis hominis,B.h)的体外培养情况.方法 采用IMDM单相培养基对B.h进行体外连续培养,比较不同pH值、血清浓度及接种量等条件下虫体生长繁殖情况及影响因素.结果 B.h在IMDM培养基中量适培养条件为:pH值7.0~8.0;新生牛血清含量大于10%;接种量不小于1×105个原虫/管;青、链霉素1万单位/mL,于37℃条件下厌氧培养,每3~6天转种1次,可以达到长期培养效果.结论 IMDM单相培养基更适合B.h的生长繁殖,可以用于诊断及体外长期培养.

  2. 人芽囊原虫病原学检查方法比较及形态观察%Comparison of the effectiveness of 3 methods of detecting Blastocystis hominis

    Institute of Scientific and Technical Information of China (English)

    胡缨; 刘登宇; 卢作超

    2013-01-01

    Objective To compare the effectiveness of 3 methods of detecting Blastocystis hominis. Methods Four hundred fecal specimens were collected from patients with diarrhea and 394 specimens were collected from healthy controls. Saline smears, iodine staining, and a modified acid-ether sedimentation technique were used to detect B. hominis and its morphology was observed. Results The 3 methods detected B. hominis in patients with diarrhea at rates of 11. 5%, 12.75%, and 20. 75%. The modified acid-ether sedimentation technique had a higher rate of detecting B. hominis than did the other two methods (χ2 = 15. 804 , P<0. 05). The technique also had a significantly higher detection threshold (parasite density) than the other two methods (χ2=144. 715, P<0. 05). In the healthy controls, the modified acid-ether sedimentation technique had the highest rate of detecting B. hominis (1. 52%) and the highest detection threshold (parasite density). The most common morphology was the vacuolar form, followed by the granular form. Conclusion An appropriate method should be chosen based on practical needs and detection conditions.%目的 比较3种人芽囊原虫病原学检查方法的检出效果. 方法 收集腹泻组400例、健康对照组394例送检粪便标本,分别采用生理盐水涂片法、碘液染色法和改良酸醚离心沉淀法检查人芽囊原虫,并观察镜下形态. 结果 腹泻患者粪便标本3种方法阳性检出率粪便为11.50%、12.75%和20.75%;改良酸醚离心沉淀法检出率高于其他两种方法(x=15.804,P<0.05),且该法检出密度亦明显高于其他两种方法(x2=144.715,P<0.05).健康对照组改良酸醚离心沉淀法阳性检出率(1.52%)和检出密度最高.粪便中人芽囊原虫最常见为空泡型,其次为颗粒型. 结论 临床检测人芽囊原虫时,应根据实际需要和检测条件选取合适的检查方法.

  3. 人芽囊原虫感染小鼠血清中IL-17水平的动态观察%Variation of level of serum IL-17 in mice infected with Blastocystis hominis

    Institute of Scientific and Technical Information of China (English)

    符瑞佳; 伍玲园; 卢作超; 唐莉莉; 刘腾; 张璠; 刘登宇

    2012-01-01

    Objective To observe the dynamic changes of IL-17 in mice infected with Blastocystis hominis. Methods 120BABIVC mice were randomly divided into experimental groups, immunosuppressant group and normal group. Four BABL/C mice from each group were randomly sacrificed on the day 2,3,4,5,6,7,8,10,12 and 14 after infection,respectively. ELISA was used to measure the serum levels of IL-17 of these mice. Results The level of serum IL-17 of immunosuppressant group and normal group maintained relatively stable throughout the experiment (P>0.05). The level of serum IL-17 of experiment group began to rise apparently after infection (P<0.05) at different stages of experiment. The level of serum IL-17 of experiment group showed a great increase on day 4,day 5 and day 6(P<0.05),and the IL-17 concentration in the serum of mice rose to a peak on the day 5. Conclusion The level of serum IL-17 in mice increased afer being infected with Blaslocystis hominis,and reached a peak on the day 5,then decreased. IL-17 may play an important role in the early infection of mice with Blastocystis hominis.%目的 观察小鼠感染人芽囊原虫后血清中白细胞介素17(IL-17)水平的动态变化,从细胞因子的角度来探讨IL-17在人芽囊原虫感染中的意义.方法 BABL/C小鼠120只随机分为实验组、免疫抑制剂组、对照组,每组各40只,三组小鼠分别于感染后第2d、3d、4d、5d、6d、7d、8d、10d、12d、14d各取4只,采用ELISA试剂盒检测小鼠血清中IL-17水平.结果 免疫抑制剂组和对照组小鼠血清中IL-17的含量在不同感染时期基本处于稳定水平(P>0.05),而感染后不同时期的实验组小鼠血清IL-17水平出现升高,差异有统计学意义(P<0.05),其中第4d、5d、6d的表达分别高于免疫抑制剂组和对照组(P<0.05),且第5d达到高峰.结论 提示了IL-17可能在人芽囊原虫早期感染中发挥了重要作用.

  4. Investigation of the prevalence of Blastocystis hominis infection among people in Nanning%南宁市城区人群人芽囊原虫感染情况调查

    Institute of Scientific and Technical Information of China (English)

    胡缨; 黎学铭; 张鸿满; 石焕焕

    2013-01-01

    Objective To investigate the prevalence of Blastocystis hominis infection among people in Nanning in order to provide scientific evidence for prevention and control measures.Methods Stool specimens were obtained from 4,263people undergoing a physical examinations or being seen at this hospital.The prevalence of B.hominis infection was investigated.Results The rate of infection withB.hominis was 7.51% (320/4,263).The rate of B.hominis infection was much higher among people who had mucopurulent bloody stool (27.33%) than those who did not (176/644; x2 =513.2%,P<0.05).Of 320 individuals who were infected,289 were infected with simple B.hominis (90.31%) and 26were infected with both B.hominis and Clonorchis sinensis (8.13%).The rates of infection did not differ significantly difference males (7.72%) and females (7.25%) (x2 =0.328,P>0.05).People with diarrhea had a higher rate of B.hominis infection (11.7 %) than healthy individuals (3.56 %) (x2 =103.6,P<0.05),but the difference was not significant (x2 =4.561,P>0.05).During the year,the highest rate of infection was in the autumn (11.26%) and then in the summer (9.72%).The rate of infection was 3.86% in 2009,7.02% in 2010,and 10.77% in 2011; the rate of infection each year differed significantly (x2 =72.533,P<0.05).Most infected individuals had a poor diet and/or poor hygiene.Of those affected,229 were symptomatic (71.56%).Conclusion Infection with B.hominis is prevalent among people in Nanning.Health education will help to block the routes of transmission and reduce the rate of infection.%目的 了解南宁市城区人群人芽囊原虫感染情况及流行规律,为采取相应的防治措施提供科学依据. 方法 对2009~2011年到医院体检或就诊的4 263例南宁市城区人群进行粪便检查,对人芽囊原虫感染情况进行分析. 结果 检出入芽囊原虫感染者320例,感染率为7.51%,其中粘液脓血便者的感染率为27.33%(176/644),

  5. Clinical analysis of Blastocystis hominis infection in 1185 patients with chronic diarrhea in 2011%慢性腹泻患者1185例感染人芽囊原虫的临床分析

    Institute of Scientific and Technical Information of China (English)

    胡缨; 李艳文; 卢作超

    2012-01-01

    目的 了解慢性腹泻患者人芽囊原虫感染和患病情况的临床特点,并分析其原因,为采取相应的防治措施提供科学依据.方法 对在我院2011年1月~ 2011年12月就诊的1185例慢性腹泻患者进行粪便检查,并对人芽囊原虫阳性患者的感染情况与实验室检测结果进行调查分析.结果 受检患者人芽囊原虫感染率为12.24%,其中有粘液脓血便的感染率明显高于其他类型腹泻者(P<0.01);不同性别、年龄间感染率差异无统计学意义(P>0.05);农民感染率最高(20.29%).实验室辅助检查中,69.66%感染患者中有血红蛋白降低,80.69%感染患者IgG、IgM、IgA均正常,86.90%感染者CD3+、CD4+表达水平降低,CD8+表达水平相对稳定,CD4+/CD8+比值下降.结论 人芽囊原虫感染是慢性腹泻的一个重要病原体,其致病作用不容忽视.%Objective To study the prevalence status and clinical features of blastocystis hominis in patients with chronic diarrhea so as to provide a basis for prevention and control measures. Methods The stool specimens of 1 185 patients with chronic diarrhea who came to our hospital during Jan.2011 to Dec.2011 were extracted; meanwhile the patients' infection situation and laboratory test results were surveyed and analyzed. Results A total of 145 out of 1 185 cases were positive with the infection rate of 12.24%. The infection rate of those with mucilage, pus and blood stool is absolutely higher than those with other kinds of diarrhea (P0.05). And the farmer's infection rate is the highest. The laboratory test results showed the level of Hgb was lower (69.66%),IgG,IgM,IgA were normal (80.69%' ). CD3+/CD4+ expression level were lower (86.90%),CD8+ expression level was relatively stable, and, CD4+/CD8+ was lower. Conclusion Blastocystis hominis is an important pathogen causing chronic diarrhea, whose pathopoiesis is not to be neglected.

  6. 巴马瑶族自治县人芽囊原虫感染情况调查%Investigation on the Infection of Blastocystis hominis in Populations in Bama Yao Autonomous County of Guangxi

    Institute of Scientific and Technical Information of China (English)

    何姗姗; 伍玲园; 刘晓泉; 石焕焕; 陈智; 张慧; 庞彩英; 李玉梅

    2013-01-01

    为了解桂西北地区巴马瑶族自治县不同人群人芽囊原虫感染情况,于2011年12月随机抽取巴马瑶族自治县5个行政村(巴马镇盘当村、凤凰乡德纳村、西山乡福厚村、燕洞乡龙威村和甲篆乡兴仁村)为调查点,收集当地居民的新鲜粪便497份,采用改良酸醚离心沉淀法,镜检定性诊断,分析不同调查点、性别、职业、年龄和民族人群芽囊原虫感染情况.结果显示,调查的497人中,人芽囊原虫感染者215例,总感染率为43.3% (215/497).不同调查点中,巴马镇盘当村的感染率最高,达55.7% (68/122),明显高于其它行政村(P<0.05),其余4行政村的感染率间差异无统计学意义(P>0.05);不同性别、职业、年龄和民族间的感染率差别均无统计学意义(P>0.05).%497 fecal specimens were collected from 5 randomly selected villages of Bama County in December 2011, and tested for Blastocystis hominis infection using improved centrifugal sedimentation with hydrochloric acid-ether. Data were analyzed by villages, gender, occupation, age groups and ethnic populations. The results showed that 215 people of 497 were positive, with a prevalence of 43.3% (215/497). Pandang village had the highest infection rate of 55.7% (68/122), significantly higher than the other villages (P0.05).

  7. Morphology of Blastocystis sp. isolated from circus animals.

    Science.gov (United States)

    Stenzel, D J; Cassidy, M F; Boreham, P F

    1993-08-01

    Blastocystis sp. is reported for the first time from faecal samples collected from a camel, a llama, a highland bull and a lion in a travelling circus. Fresh faecal specimens were examined by light and electron microscopy, and vacuolar and cyst forms of similar morphology were present in all three ungulates. These cells were smaller than cultured vacuolar cells of Blastocystis hominis isolated from humans and contained only a single vacuole in comparison to the multivacuolar cell found in fresh human faeces. The taxonomic relationship of Blastocystis isolated from humans and ungulates remains to be determined. The number of parasites present in the lion sample was too small to make valid comparisons.

  8. Expressions and significance of IL-17 and IL-23 in intestinal mucosa of mice infected with Blastocystis hominis%人芽囊原虫感染小鼠肠黏膜IL-17和IL-23的表达及意义

    Institute of Scientific and Technical Information of China (English)

    伍玲园; 符瑞佳; 卢作超; 唐莉莉; 张璠; 刘登宇

    2012-01-01

    目的 观察人芽囊原虫(Blastocystis hominis)感染小鼠肠道组织中白细胞介素17(IL-17)和IL-23的表达情况.方法 30只BABL/c小鼠随机分为实验组、免疫抑制剂组和对照组,实验组和免疫抑制剂组小鼠腹腔注射地塞米松2 mg/只,日1次,连续5d;对照组小鼠腹腔注射生理盐水0.2 ml/只.实验组每鼠经口感染107个人芽囊原虫,免疫抑制组和对照组每鼠经口灌服0.5 ml生理盐水.感染人芽囊原虫5d后,剖杀小鼠,分别取十二指肠、空肠、回肠和结肠制作成组织切片,HE染色检查小鼠肠黏膜病变,免疫组化法检测各肠黏膜中IL-17和IL-23的表达水平.结果 HE染色显示,肠道黏膜有不同程度炎症改变.IL-17和IL-23在实验组小鼠不同肠黏膜中的表达均显著高于免疫抑制剂组和对照组(P<0.05),而免疫抑制剂组与对照组IL-17和IL-23在小鼠不同肠黏膜中的表达差异无统计学意义(P> 0.05).IL-17在实验组小鼠十二指肠、空肠、结肠黏膜中的表达水平均显著高于回肠黏膜(P<0.05),IL-23在实验组小鼠十二指肠、空肠黏膜中的表达水平显著高于回肠和结肠黏膜(P<0.05).结论 IL-17和IL-23参与人芽囊原虫感染的免疫反应,且IL-17与IL-23在人芽囊原虫感染的免疫应答过程中有一定的相互调节作用.%Objective To study the expressions of interleukin-17 (IL-17) and interleukin-23 (IL-23) in the intestinal mucosa of BABL/C mice infected with Blastocystis hominis. Methods A total of 30 BABL/C mice were randomly divided into different groups: an experimental group, an immunosuppressant group and a normal group. Each mouse of the experimental group and im-munosuppressant group was administered intraperieneally with dexamethasone (2 mg, gd, for 5 days) and one of the control group was given physiological saline (0.2 ml). In the experimental group, each mouse was infected with Blastocystis hominis (107 parasites per 0.5 ml) by the intragastric

  9. 人芽囊原虫生物学特性——形态特征、繁殖方式及与细菌的关系研究%Study on the biological characteristic of Blastocystis hominis:morphology, mode of reproduction and the relation to bacteria

    Institute of Scientific and Technical Information of China (English)

    乔继英; 张旭; 魏志超; 杨珺华; 李亚青; 张荣

    2006-01-01

    目的 观察人芽囊原虫(Blastocystis hominis,B.h)的生殖方式,对B.h与细菌的关系及相互间微环境的影响进行初步探索.方法 分别对新鲜粪便标本和RPMI 1640培养基中B.h,经碘液染色和苏木素染色观察其形态,对B.h阳性的粘液便、粘液血便进行肠道常见致病菌的培养和鉴定,并通过B.h与大肠埃希菌共培养实验,观察两者相互作用.结果 本研究确证了四种B.h繁殖方式:二分裂生殖、内二芽生殖、复分裂增殖及出芽生殖.半数B.h阳性标本未检出已知肠道致病菌.B.h对大肠埃希菌有一定抑制作用.结论 B.h存在多种繁殖方式;B.h可能单独致病,其致病机制与微生态改变有关.

  10. 人芽囊原虫病患者肠黏膜表皮生长因子水平及其临床意义%Intestinal mucosa of epidermal growth factor in patients with Blastocystis hominis infection and its clinical significance

    Institute of Scientific and Technical Information of China (English)

    俞开敏

    2011-01-01

    目的 检测人芽囊原虫感染者肠黏膜表皮生长因子(EGF)的水平,探讨EGF与肠黏膜损伤程度的关系.方法 感染组为确诊人芽囊原虫感染者44例,对照组为健康体检者40例,均经电子肠镜检查肠黏膜病变情况,并用ELISA法检测肠黏膜组织匀浆EGF水平.结果 电子肠镜检查可见人芽囊原虫感染者的肠黏膜有不同程度病变,表现为充血、水肿、糜烂、溃疡等.感染组EGF为(559.60±178.5)pg/ml,对照组为(977.58±215.5)pg/ml,差异有统计学意义(t=9.7136,P<0.01).且EGF水平随着肠黏膜病变严重程度的增加而逐渐降低.结论 人芽囊原虫感染能引起患者肠黏膜的病理改变及肠黏膜EGF水平降低.人芽囊原虫感染所致肠黏膜损伤可能与原虫的侵入及黏膜中EGF的减少有关.EGF在人芽囊原虫病肠道损伤的修复中起重要作用.%Objective To detect epidermal growth factor (EGF) in intestinal mucosa in patients with Blastocystis hominis infection and explore the relationship between EGF and intestinal mucosa lesions. Methods Forty-four patients who were diagnosed with B. Hominis infection served as the infected group and 40 healthy persons served as the control group. Endoscopy and intestinal biopsy were performed to observe pathological changes in the intestine. The patients'and the controlsintestinal tissue homogenate were tested for EGF with ELISA assays. Results Intestinal mucosa lesions were observed in all patients infected with B. Hominis. Pathological manifestations included mucosal congestion, edema, erosion, and ulceration. The level of EGF in the infected group was (559. 60±178. 5) pg/ml, and that in the control was (977. 58±215. 5) pg/ml. The difference between the two was significant (t=9. 7136,P<0. 01). Furthermore, the level of EGF gradually decreased with the severity of intestinal mucosa lesions. Conclusion B. Hominis infection can cause intestinal mucosa lesions and reduce levels of EGF in the intestinal

  11. Effect of intestinal mucosa for immunosuppressed mice on different infection pathways of Blastocystis hominis%人芽囊原虫不同感染途径对免疫抑制小鼠肠黏膜的影响

    Institute of Scientific and Technical Information of China (English)

    王东; 薛长贵

    2012-01-01

    OBJECTIVE To discuss the parasitism and the injury of intestinal mucosa (B.hominis) through oral and rectum. METHODS 60 Kongming mouse were randomly immunosuppressed and divided into 6 groups. Four groups were infected with 15×103, 20×103 dose of parasites through oral and rectum, respectively. The remaining two was designated as control group. RESULTS B.hominis mainly parasitized in enteric cavity and on the surface of mucosa of ileocolon and cecum through oral and rectum. The microscope and the electron microscope showed that the histological examination of tissue of caecum revealed light pathological alteration in the group through oral infection, while obvious pathological alteration showed in the intestinal mucosa in the group through rectum infection. There was no obvious distinguish of infection dose. The mouse in control group had no parasites and the structure of mucosa kept normal. CONCLUSION Pathological changes of intestinal mucosa have relations with infection pathway. The condition of pathology become more obvious through rectum infection. B.hominis mainly locates in enteric cavity and on the surface of mucosa of ileocolon.%目的 探讨人芽囊原虫(B.hominis)经口和经直肠感染免疫功能低下小鼠后在肠道内寄生和对肠黏膜的损伤情况.方法 将昆明小鼠60只应用免疫抑制剂制备为免疫功能低下小鼠后,随机分为6组.其中4组分别经口和直肠感染15×103、20×103剂量的虫体,其他两组为阴性对照组.感染后d2观察各组小鼠症状并粪便检查感染情况.感染7d后处死动物,分段将肠内容物涂片检查虫体寄生情况,同时取回盲部肠黏膜观察光镜下的病理结构改变和电镜的超微病理变化.结果 经口和直肠感染均显示该虫主要寄生在回盲部和盲肠,主要附着在肠腔和肠黏膜表面.光学和电子显微镜检查显示经口感染小鼠肠黏膜组织病理变化较轻,经直肠感染小鼠肠黏膜病理改变较重,不同感

  12. Molecular epidemiology of Blastocystis

    Directory of Open Access Journals (Sweden)

    Fadime Eroğlu

    2015-12-01

    Full Text Available Blastocystis pathogenicity and classification was newly illuminated with molecular genetic studies and recently the parasite was found in the focus of many researchers. Several molecular methods such as; polymerase chain reaction (PCR, PCR-restriction fragment length polymorphism, random amplified polymorphic DNA, real-time polymerase chain reaction and DNA sequencing analyses can be used in genotyping of Blastocystis. Blastocystis parasites may cause diarrhea, abdominal pain, bloating, gas, irritability, anorexia, cramps, vomiting, dehydration, insomnia, nausea, loss of appetite, weight loss, fatigue symptoms and also could be asymptomatic cases. In this review, it was aimed to summarize the associations between Blastocystis subtypes and pathogenicity.

  13. IL-5及嗜酸粒细胞与人芽囊原虫病患者肠黏膜损伤关系的探讨%The relationship between IL-5, eosinophils and intestinal mucosa lesions in the patients with Blastocystis hominis infection

    Institute of Scientific and Technical Information of China (English)

    俞开敏

    2011-01-01

    目的 探讨IL-5及嗜酸性粒细胞与人芽囊原虫病患者肠黏膜损伤的关系.方法 实验组为确诊为人芽囊原虫感染的30例患者,对照组为30例健康体检者.均做电子肠镜检查,用ELISA法检测肠黏膜组织匀浆中的IL-5水平.实验组取病变黏膜做病理检查,HE染色病理切片,并观察嗜酸性粒细胞的浸润情况.结果 经电子肠镜检查,30例人芽囊原虫病患者均出现不同程度的肠道病变,表现为充血、水肿、糜烂、溃疡等.病理学观察病变组织有嗜酸性粒细胞浸润.实验组肠黏膜组织匀浆IL-5(45.75±24.1)pg/ml,对照组为(19.90±7.6)pg/ml,差异有统计学意义(t=5.603 0,P<0.01),且人芽囊原虫感染者肠黏膜组织匀浆IL-5水平随着浸润嗜酸性粒细胞数目增加而逐渐升高,人芽囊原虫感染度与嗜酸性粒细胞浸润数目呈正相关(rs=0.771 1,P<0.01).结论 人芽囊原虫感染能引起患者肠黏膜的病理改变,嗜酸性粒细胞浸润及细胞因子IL-5水平升高.IL-5和嗜酸性粒细胞在人芽囊原虫感染所致的肠黏膜损伤中起重要作用,且IL-5是人芽囊原虫感染引起免疫反应导致肠黏膜损伤的重要细胞因子之一.%Objective To investigate the relationship between IL-5, eosinophils, and intestinal mucosa lesions in patients with Blastocystis hominis infection. Methods Thirty patients who were diagnosed with B. hominis infection served as the experimental group and 30 healthy persons served as the control group. Endoscopy and intestinal biopsy were performed to observe pathologic changes in the intestine. The patients' and the controls' intestinal tissue homogenate were tested for IL-5 with ELISA assays. The patients' eosinophils in pathological sections were ascertained using HE staining. Results Intestinal mucosal lesions were found in all patients infected with B. hominis. The pathological manifestations included mucosal congestion, edema, erosion, and ulceration. Eosinophils

  14. Axenic culture of reptilian Blastocystis isolates in monophasic medium and speciation by karyotypic typing.

    Science.gov (United States)

    Singh, M; Ho, L C; Yap, A L; Ng, G C; Tan, S W; Moe, K T; Yap, E H

    1996-01-01

    The growth of axenic reptilian isolates of Blastocystis in Iscove's modified Dulbecco's medium (IMDM) was studied and the morphology of the parasite was examined by phase-contrast microscopy. The chromosomal patterns of these reptilian isolates of Blastocystis were examined by pulsed-field gel electrophoresis (PFGE) and compared with those of B. hominis and B. lapemi, a sea snake Blastocystis. IMDM with 10% horse serum supported excellent growth of the reptilian Blastocystis isolates. The parasites from all the isolates were predominantly vacuolar, but multivacuolar and amoeboid forms were also seen. Amoeboid forms with rather elongate pseudopodia were also observed. There were some differences in size, morphology, and growth characteristics in the different reptilian isolates. The karyotypic patterns of the Blastocystis isolates from tortoise, iguana, and python were distinctly different from one another and from those obtained with B. hominis and B. lapemi. On the basis of the above-mentioned differences in chromosomal patterns, the tortoise, iguana, and python isolates are described as new species, viz., B. geocheloni sp. nov. from Geochelone carbonaria (red-footed tortoise), B. cycluri sp. nov. from Cyclura cornuta (rhino iguana), and B. pythoni sp. nov. from Python reticulatus (reticulated python). PMID:8825212

  15. [Blastocystis infection in symptomatic patients at the Regional Hospital of Temuco, Chile].

    Science.gov (United States)

    Biolley, M A; Oberg, C

    1993-01-01

    At the Regional Hospital of Temuco, 670 adult and children patients, with intestinal complaints, were studied to determine the prevalence of infection by enteroparasites or commensals in a general way, and by Blastocystis hominis in a particular attempt. A coproparasitological study was carried out using a modified Telemann method. Infection by parasites and/or commensals was demonstrated in 446 (66.6%) patients. The most frequent agent was B. hominis (35.8%) followed by Giardia intestinalis (22.5%) among the protozoa. The frequencies of helminths and commensals were 7.7 and 42.5% respectively. The endemic characteristics of intestinal infections by parasites was ratified and the prevalence of infection by B. hominis was proved to be higher in comparison to other zones of the country. PMID:8110370

  16. Recent developments in Blastocystis research.

    Science.gov (United States)

    Clark, C Graham; van der Giezen, Mark; Alfellani, Mohammed A; Stensvold, C Rune

    2013-01-01

    Blastocystis is a common parasite of the human large intestine but has an uncertain role in disease. In this review, we appraise the published evidence addressing this and its weaknesses. Genetic diversity studies have led to the identification of numerous subtypes (STs) within the genus Blastocystis and, recently, methods for studying variation within STs have been developed, with implications for our understanding of host specificity. The geographic distribution of STs is summarised and the impact this may have on investigations into the role of the organism in disease is discussed. Finally, we describe the organelle and nuclear genome characteristics and look to future developments in the field. PMID:23548084

  17. Mysterious chronic urticaria caused by Blastocystis spp.?

    Science.gov (United States)

    Lepczyńska, Małgorzata; Chen, Wen-Chieh; Dzika, Ewa

    2016-03-01

    Species of the genus Blastocystis, which are single-cell, intestinal protozoan parasites of humans and animals, remain mysterious, with unclear clinical and epidemiologic significance. In recent years, many researchers have suggested a possible connection between Blastocystis spp. infection and chronic urticaria. In the present article, we review the literature and discuss the possible associations between the clinical symptomatology and pathogenicity of this organism in terms of its subtypes, morphologic forms, genetic diversity, and interactions with other intestinal microbiota. PMID:26469206

  18. Phenotypic variation in Blastocystis sp. ST3

    OpenAIRE

    Ragavan, Nanthiney Devi; Govind, Suresh Kumar; Chye, Tan Tian; Mahadeva, Sanjiv

    2014-01-01

    Background Blastocystis, is one of the most common human intestinal protozoan, which has many conflicting reports on its pathogenic role. Gut conditions which obviously varies in asymptomatic individuals, symptomatic and irritable bowel syndrome (IBS) patients in terms of gut flora, pH, osmotic pressure and water potentials could play an important role in its pathogenicity. The present study is the first study to investigate phenotypic characteristics of Blastocystis sp. ST3 isolated from asy...

  19. Blastocystis sp. from food animals in India

    OpenAIRE

    Sreekumar, C.; Selvaraj, J.; Gomathinayagam, S.; Thangapandiyan, M.; Ravikumar, G.; Roy, Parimal; C Balachandran

    2013-01-01

    Blastocystis, a zoonotic protozoan found in the intestinal tracts of a wide range of animals, has not been reported from non-human hosts from India so far. Organisms indistinguishable from Blastocystis sp. were identified in the Giemsa stained intestinal scrapings collected from carcasses of piglet and poultry that were brought for necropsy to the Central University Laboratory, Chennai. The ‘central vacuole forms’ of the parasite, with number of nuclei ranging from 1 to 12 were identified. Th...

  20. BLASTOCYSTIS SP. AND BLASTOCYSTIS RATTI IN A BRAZILIAN PORCUPINE (COENDOU PREHENSILIS) WITH DIARRHEA.

    Science.gov (United States)

    Goe, Alexandra M; Heard, Darryl J; Easley, J Roger; Weeden, Amy L; Childress, April L; Wellehan, James F X

    2016-06-01

    A hand-raised, 5-mo-old, intact male Brazilian porcupine (Coendou prehensilis) was evaluated for chronic diarrhea, failure to thrive, and anorexia. On presentation the porcupette was dull, dehydrated, and passing yellow, malodourous, watery diarrhea. Cytologic examination of feces revealed a large number of organisms, morphologically consistent with Blastocystis. Blastocystis polymerase chain reaction (PCR) performed on feces was positive. Direct sequencing on two sequential samples confirmed the presence of Blastocystis ratti and a novel Blastocystis sequence. The porcupette was treated supportively, which included a 4-wk metronidazole course. Diarrhea resolved within 2 wk of treatment, and the animal's growth rate dramatically improved. Recheck PCR was negative for Blastocystis. Although an important and controversial cause of diarrhea in immunocompromised humans, this organism is not well recognized as a potential pathogen and zoonosis in zoo animals. Clinicians should be aware of the potential for disease associated with this organism, especially in immunocompromised animals. PMID:27468042

  1. A retrospective metagenomics approach to studying Blastocystis.

    Science.gov (United States)

    Andersen, Lee O'Brien; Bonde, Ida; Nielsen, Henrik Bjørn; Stensvold, Christen Rune

    2015-07-01

    Blastocystis is a common single-celled intestinal parasitic genus, comprising several subtypes. Here, we screened data obtained by metagenomic analysis of faecal DNA for Blastocystis by searching for subtype-specific genes in coabundance gene groups, which are groups of genes that covary across a selection of 316 human faecal samples, hence representing genes originating from a single subtype. The 316 faecal samples were from 236 healthy individuals, 13 patients with Crohn's disease (CD) and 67 patients with ulcerative colitis (UC). The prevalence of Blastocystis was 20.3% in the healthy individuals and 14.9% in patients with UC. Meanwhile, Blastocystis was absent in patients with CD. Individuals with intestinal microbiota dominated by Bacteroides were much less prone to having Blastocystis-positive stool (Matthew's correlation coefficient = -0.25, P metagenomics approach. The study serves as an example of how it is possible to retrospectively investigate microbial eukaryotic communities in the gut using metagenomic datasets targeting the bacterial component of the intestinal microbiome and the interplay between these microbial communities.

  2. Diversity of Blastocystis subtypes in dogs in different geographical settings

    DEFF Research Database (Denmark)

    Wang, Wenqi; Cuttell, Leigh; Bielefeldt-Ohmann, Helle;

    2013-01-01

    Background: Blastocystis is a ubiquitous, globally distributed intestinal protist infecting humans and a wide range of animals. Several studies have shown that Blastocystis is a potentially zoonotic parasite. A 1996 study reported a 70% Blastocystis prevalence in Brisbane pound dogs while another...... study found that pet dogs/cats of 11 symptomatic Blastocystis infected patients harboured at least one Blastocystis subtype (ST) in common with the patient. These results raised the possibility that dogs might be natural hosts of Blastocystis. In this study, we aimed to investigate this hypothesis...... by estimating the prevalence of Blastocystis carriage and characterising the diversity of STs in dogs from three different environmental settings and comparing these STs with the range that humans harbour. Methods: Two hundred and forty faecal samples from dogs from three different geographical regions...

  3. 人芽囊原虫病37例%Blastocystis Hominis Infections in 37 Children

    Institute of Scientific and Technical Information of China (English)

    唐清; 王琳琳; 刘登宇; 卢作超

    2009-01-01

    目的 了解儿童人芽囊原虫(BH)病感染的临床表现及发病特点.方法 选择2006年1月-2007年12月在广西医科大学第一附属医院儿科住院的患儿250例,询问病史,收集患儿新鲜大便,用9 g/L盐水直接涂片法检查BH,对疑似阳性标本再用碘染色法及三色法镜检鉴别并计数.对于感染BH的患儿予甲硝唑治疗1个疗程(7~10 d)后,复查粪便BH感染度.结果 250例住院患儿共检出BH 37例,检出率14.80%.男26例,女11例,男女感染率分别为15.12%和14.10%,二者无统计学差异(P>0.05).出现消化道症状者34例(91.89%),腹痛是最常见症状(19例,51.35%),多表现为慢性腹痛,部位多位于脐周,其次为腹泻(15例,40.54%),粪便性状以水样便为多,多为急性腹泻,慢性腹泻较少.甲硝唑治疗1个疗程,32例(86.49%)治愈,4例治疗2个疗程治愈,1例治疗3个疗程后好转.结论 BH感染是儿童常见的肠道寄生虫,可引起以腹痛、腹泻为主的消化道症状,甲硝唑为治疗BH病的首选药物.

  4. Recent advances in Blastocystis hominis research%人芽囊原虫的研究进展

    Institute of Scientific and Technical Information of China (English)

    贾中伟; 沈继龙

    2006-01-01

    人芽囊原虫是一种常见的人和哺乳动物肠道寄生原虫.因在有症状与无症状个体均可发现,故人芽囊原虫的致病性一直存在争议.该文从生物学、流行病学、分子生物学、免疫学、临床表现与治疗、及实验室检查等几方面简要概述近年来国内外对其研究的进展.

  5. Blastocystis sp. Infection Mimicking Clostridium Difficile Colitis

    Science.gov (United States)

    Gil, Gaby S.; Chaudhari, Shobhana; Shady, Ahmed; Caballes, Ana; Hong, Joe

    2016-01-01

    We report an unusual case of severe diarrhea related to Blastocystis sp. infection in a patient with end stage renal disease on hemodialysis. The patient was admitted due to profuse diarrhea associated with fever and leukocytosis. Pertinent stool work-up such as leukocytes in stool, stool culture, clostridium difficile toxin B PCR, and serology for hepatitis A, hepatitis B, and hepatitis C and cytomegalovirus screening were all negative. Ova and parasite stool examination revealed Blastocystis sp. The patient was given intravenous metronidazole with clinical improvement by day three and total resolution of symptoms by day ten. PMID:27247810

  6. Blastocystis sp. Infection Mimicking Clostridium Difficile Colitis.

    Science.gov (United States)

    Gil, Gaby S; Chaudhari, Shobhana; Shady, Ahmed; Caballes, Ana; Hong, Joe

    2016-01-01

    We report an unusual case of severe diarrhea related to Blastocystis sp. infection in a patient with end stage renal disease on hemodialysis. The patient was admitted due to profuse diarrhea associated with fever and leukocytosis. Pertinent stool work-up such as leukocytes in stool, stool culture, clostridium difficile toxin B PCR, and serology for hepatitis A, hepatitis B, and hepatitis C and cytomegalovirus screening were all negative. Ova and parasite stool examination revealed Blastocystis sp. The patient was given intravenous metronidazole with clinical improvement by day three and total resolution of symptoms by day ten. PMID:27247810

  7. Blastocystis tropism in the pig intestine

    Science.gov (United States)

    Blastocystis subtype 5, a subtype known to infect humans, was detected by molecular methods in the feces of 36 naturally infected market age pigs. At necropsy, 6 heavily infected pigs were selected to determine the tropism of the infection within the gastrointestinal tract. Because so little is know...

  8. Clinical significance and taxonomy of Actinobacillus hominis

    DEFF Research Database (Denmark)

    Friis-Møller, Alice; Christensen, J J; Fussing, V;

    2001-01-01

    Clinical findings in 36 immunosuppressed patients with lower respiratory tract infection or bacteremia with Actinobacillus hominis are described. Animal contact was only recorded for three patients; nine patients died despite appropriate antimicrobial treatment. Although infections with this micr...

  9. Location and Pathogenic Potential of Blastocystis in the Porcine Intestine

    OpenAIRE

    Wenqi Wang; Helle Bielefeldt-Ohmann; Traub, Rebecca J; Leigh Cuttell; Helen Owen

    2014-01-01

    Blastocystis is an ubiquitous, enteric protozoan of humans and many other species. Human infection has been associated with gastrointestinal disease such as irritable bowel syndrome, however, this remains unproven. A relevant animal model is needed to investigate the pathogenesis/pathogenicity of Blastocystis. We concluded previously that pigs are likely natural hosts of Blastocystis with a potentially zoonotic, host-adapted subtype (ST), ST5, and may make suitable animal models. In this stud...

  10. Blastocystis in a Gut Microbia Context and Aspects of Blastocystis Genomics

    DEFF Research Database (Denmark)

    Andersen, Lee Poul O'Brien

    Blastocystis is a single-celled intestinal parasite of undetermined clinical and public health importance comprising 17 subtypes (species), of which 9 have been found in humans. In the first study of this thesis, general trends in Blastocystis research are accounted for, and the general assumption...... that Blastocystis is pathogenic is challenged. For instance, several studies have explored the prevalence and molecular epidemiology of Blastocystis in various groups of patients suffering gastrointestinal symptoms, apparently ignoring the necessity of inclusion of suitable control groups of healthy individuals...

  11. Location and pathogenic potential of Blastocystis in the porcine intestine.

    Directory of Open Access Journals (Sweden)

    Wenqi Wang

    Full Text Available Blastocystis is an ubiquitous, enteric protozoan of humans and many other species. Human infection has been associated with gastrointestinal disease such as irritable bowel syndrome, however, this remains unproven. A relevant animal model is needed to investigate the pathogenesis/pathogenicity of Blastocystis. We concluded previously that pigs are likely natural hosts of Blastocystis with a potentially zoonotic, host-adapted subtype (ST, ST5, and may make suitable animal models. In this study, we aimed to characterise the host-agent interaction of Blastocystis and the pig, including localising Blastocystis in porcine intestine using microscopy, PCR and histopathological examination of tissues. Intestines from pigs in three different management systems, i.e., a commercial piggery, a small family farm and a research herd (where the animals were immunosuppressed were examined. This design was used to determine if environment or immune status influences intestinal colonisation of Blastocystis as immunocompromised individuals may potentially be more susceptible to blastocystosis and development of associated clinical signs. Intestines from all 28 pigs were positive for Blastocystis with all pigs harbouring ST5. In addition, the farm pigs had mixed infections with STs 1 and/or 3. Blastocystis organisms/DNA were predominantly found in the large intestine but were also detected in the small intestine of the immunosuppressed and some of the farm pigs, suggesting that immunosuppression and/or husbandry factors may influence Blastocystis colonisation of the small intestine. No obvious pathology was observed in the histological sections. Blastocystis was present as vacuolar/granular forms and these were found within luminal material or in close proximity to epithelial cells, with no evidence of attachment or invasion. These results concur with most human studies, in which Blastocystis is predominantly found in the large intestine in the absence of

  12. Location and pathogenic potential of Blastocystis in the porcine intestine.

    Science.gov (United States)

    Wang, Wenqi; Bielefeldt-Ohmann, Helle; Traub, Rebecca J; Cuttell, Leigh; Owen, Helen

    2014-01-01

    Blastocystis is an ubiquitous, enteric protozoan of humans and many other species. Human infection has been associated with gastrointestinal disease such as irritable bowel syndrome, however, this remains unproven. A relevant animal model is needed to investigate the pathogenesis/pathogenicity of Blastocystis. We concluded previously that pigs are likely natural hosts of Blastocystis with a potentially zoonotic, host-adapted subtype (ST), ST5, and may make suitable animal models. In this study, we aimed to characterise the host-agent interaction of Blastocystis and the pig, including localising Blastocystis in porcine intestine using microscopy, PCR and histopathological examination of tissues. Intestines from pigs in three different management systems, i.e., a commercial piggery, a small family farm and a research herd (where the animals were immunosuppressed) were examined. This design was used to determine if environment or immune status influences intestinal colonisation of Blastocystis as immunocompromised individuals may potentially be more susceptible to blastocystosis and development of associated clinical signs. Intestines from all 28 pigs were positive for Blastocystis with all pigs harbouring ST5. In addition, the farm pigs had mixed infections with STs 1 and/or 3. Blastocystis organisms/DNA were predominantly found in the large intestine but were also detected in the small intestine of the immunosuppressed and some of the farm pigs, suggesting that immunosuppression and/or husbandry factors may influence Blastocystis colonisation of the small intestine. No obvious pathology was observed in the histological sections. Blastocystis was present as vacuolar/granular forms and these were found within luminal material or in close proximity to epithelial cells, with no evidence of attachment or invasion. These results concur with most human studies, in which Blastocystis is predominantly found in the large intestine in the absence of significant organic

  13. Epidemiological Aspects of Blastocystis Colonization in Children in Ilero, Nigeria.

    Science.gov (United States)

    Poulsen, Casper S; Efunshile, Akinwale M; Nelson, Jenna A; Stensvold, Christen R

    2016-07-01

    This study aimed to elucidate aspects of the epidemiology of Blastocystis in Nigerian school children, including the distribution of subtypes (STs) and ST alleles. A total of 199 genomic DNAs extracted from fecal samples from 199 Nigerian children aged 2-14 years were tested by real-time polymerase chain reaction for Blastocystis Positive DNAs were submitted to barcoding by PCR and sequencing to obtain information on STs and ST alleles. A total of 167 (84%) samples were positive for Blastocystis, with prevalence increasing by age. No association between Blastocystis colonization and gender (P = 0.51) or type/presence of toilet facilities (P = 0.21) was observed. Blastocystis carriers were more prone to using water collected from wells than from sachets (P = 0.0044). Moreover, Blastocystis positivity was associated with positivity for fecal-orally transmitted protozoa (P = 0.018) and helminths (P Blastocystis colonization and malaria infection was observed (P Blastocystis colonization. ST data were available for 127/167 (76%) samples. Fifty-one children were positive for ST1, while 42 and 33 children were colonized with ST2 and ST3, respectively; a single case of ST7 was observed. By and large, the ST alleles identified for ST1 and ST2 did not differ from those observed in humans in other regions of the world; meanwhile, the distribution of ST3 alleles was remarkably distinct and potentially specific to humans in sub-Saharan Africa. PMID:27139454

  14. Pharmacokinetics of Moxifloxacin in an Infant with Mycoplasma hominis Meningitis

    OpenAIRE

    Watt, Kevin M; Massaro, Matthew M; Smith, Brian; Cohen-Wolkowiez, Michael; Benjamin, Daniel K; Laughon, Matthew M

    2012-01-01

    Treatment of Mycoplasma hominis meningitis in infants is limited by a lack of consensus regarding therapy and limited pharmacokinetic data for agents to which M. hominis is susceptible. We report the successful treatment of a premature infant with M. hominis meningitis with doxycycline and moxifloxacin and provide a pharmacokinetic profile of moxifloxacin.

  15. The Antiapoptic Effects of Hominis Placenta Extract

    Directory of Open Access Journals (Sweden)

    Jung-Chul Seo

    2001-02-01

    Full Text Available Purpose. Free radicals are implicated in the pathophysiology of aging, ischemic injury and neurodegenerative disorders. To deform]no whether Hominis Placenta extract prevents H2O2-induced apoptosis, we have performed morphological and biochemical analyses for the detection of apoptotic phenomena in the pineal tumor cell line PGT-beta We have also peformed cytochemical and immunocytochemical analyses for the detection of changes in nitric oxide synthase (NOS activity and estimated the expression . of apoptotic genes using reverse transcription-polymerase chain reaction (RT-PCR Methods. PGT-beta cells were pretreated with Hominis Placenta extracts (0,10-2/ug/ml for 2 hours and then exposed to H2O2 (0, 50uM for 3 hours. Appearance of apoptotic characteristics were monitored using 4, 6-diamidino-2-phenylindole dihydrochloride (DAPI staining assay, terminal deoxynucleotidyl transferase-mediated dUTP-digoxigenin nick end labeling (TUNEL assay and flow cytometric analysis. NOS activity was measured by NADPH-diaphorase cytochemistry. Expression of inducible NOS (iNOS and nuclear factor kappa B (NF k B was assessed via immunocytochemistry. The expression of apoptotic genes was examined by RT-PCR. Results. After 3 flours of exposure to H2O2, it was shown that PGT-beta cells treated with H2O2(50 uM exhibit classical apoptotic features and increases in NOS activity and caspase-3 expression. Treatment with Hominis Placenta extract resulted in a reduced occurrence of apoptotic features. DAPI staining, TUNEL and flow cytometric assays revealed decreases in the occurrence of nuclear fragmentation and in the sub-Gl fraction in the PGT-beta cells treated with Hominis Placenta extract. Cells treated with Hominis Placenta extract also showed lower activity of NADPH-diaphorase and immunoreactivities of both iNOS and NF k B than those of H2O2-treated cells which were not treated with Hominis Placenta extract. By RT-PCR, it was shown that the level of caspase-3 m

  16. Clinical significance and taxonomy of Actinobacillus hominis

    DEFF Research Database (Denmark)

    Friis-Møller, Alice; Christensen, J J; Fussing, V;

    2001-01-01

    microorganism seem to be rare, the fact that 37 of 46 strains characterized in this study have been found in Copenhagen indicates that under-reporting may occur. A. hominis is phenotypically relatively homogeneous but can be difficult to differentiate from other Actinobacillus species unless extensive...

  17. Molecular design of Mycoplasma hominis Vaa adhesin

    DEFF Research Database (Denmark)

    Boesen, Thomas; Fedosova, Natalya U.; Kjeldgaard, Morten;

    2001-01-01

    The variable adherence-associated (Vaa) adhesin of the opportunistic human pathogen Mycoplasma hominis is a surface-exposed, membrane-associated protein involved in the attachment of the bacterium to host cells. The molecular masses of recombinant 1 and 2 cassette forms of the protein determined...

  18. Homo homini : [luuletused] / Valeria Ränik

    Index Scriptorium Estoniae

    Ränik, Valeria, 1964-

    2004-01-01

    Sisu: Homo homini ; "Tegelikult on täiuslik kõik..." ; Mõned kõned ; "Vihmadest lekib lagi..." ; "Sündisid siia, et maksta maksu..." ; "Mingis kohas, mingil ajal..." ; "Emajõgi, Amme jõgi..." ; Kaktus ; Eraelamus ; "Minevikule vesi peale..."

  19. OCCURRENCE OF Blastocystis spp. IN UBERABA, MINAS GERAIS, BRAZIL.

    Science.gov (United States)

    Cabrine-Santos, Marlene; Cintra, Eduardo do Nascimento; do Carmo, Rafaela Andrade; Nascentes, Gabriel Antônio Nogueira; Pedrosa, André Luiz; Correia, Dalmo; Oliveira-Silva, Márcia Benedita de

    2015-01-01

    Intestinal parasites are a problem for public health all over the world. The infection with Blastocystis, a protozoan of controversial pathogenicity, is one of the most common among them all. In this study, the occurrence of intestinal parasites, with emphasis on Blastocystis, in patients at the Universidade Federal do Triângulo Mineiro was investigated in Uberaba (MG) through microscopy of direct smears and fecal concentrates using Ritchie's method. Feces of 1,323 patients were examined from April 2011 to May 2012. In 28.7% of them at least one intestinal parasite was identified, and the most frequent organisms were Blastocystis spp. (17.8%) and Giardia intestinalis (7.4%). The occurrence of parasitism was higher in children aged 6 -10 years old, and the infection with Blastocystis spp. was higher above the age of six (p spp. was observed in 5.4% and 12.2% of the patients, respectively. Regarding patients with diarrheic feces, 8% revealed unique parasitism of Blastocystis spp. Other intestinal parasites observed in children were Ascaris lumbricoides (0.3%) and Entamoeba histolytica/dispar/moshkovskii (1.4%). The Ritchie's method was more sensitive (92.8%) when compared to direct microscopy (89.8%), with high agreement between them (97.7%, kappa = 0.92). In conclusion, the occurrence of Blastocystis spp. in Uberaba is high and the presence of diarrheic feces with exclusive presence of the parasite of Blastocystis spp. was observed. PMID:26200960

  20. OCCURRENCE OF Blastocystis spp. IN UBERABA, MINAS GERAIS, BRAZIL

    Directory of Open Access Journals (Sweden)

    Marlene CABRINE-SANTOS

    2015-06-01

    Full Text Available Intestinal parasites are a problem for public health all over the world. The infection with Blastocystis, a protozoan of controversial pathogenicity, is one of the most common among them all. In this study, the occurrence of intestinal parasites, with emphasis on Blastocystis, in patients at the Universidade Federal do Triângulo Mineiro was investigated in Uberaba (MG through microscopy of direct smears and fecal concentrates using Ritchie’s method. Feces of 1,323 patients were examined from April 2011 to May 2012. In 28.7% of them at least one intestinal parasite was identified, and the most frequent organisms were Blastocystis spp. (17.8% and Giardia intestinalis (7.4%. The occurrence of parasitism was higher in children aged 6 -10 years old, and the infection with Blastocystis spp. was higher above the age of six (p < 0.001. The exclusive presence of G. intestinalis and of Blastocystis spp. was observed in 5.4% and 12.2% of the patients, respectively. Regarding patients with diarrheic feces, 8% revealed unique parasitism of Blastocystis spp. Other intestinal parasites observed in children were Ascaris lumbricoides (0.3% and Entamoeba histolytica/dispar/moshkovskii (1.4%. The Ritchie’s method was more sensitive (92.8% when compared to direct microscopy (89.8%, with high agreement between them (97.7%, kappa = 0.92. In conclusion, the occurrence of Blastocystis spp. in Uberaba is high and the presence of diarrheic feces with exclusive presence of the parasite of Blastocystis spp. was observed.

  1. Laboratory diagnosis of Blastocystis spp. in diarrheic patients

    OpenAIRE

    Elghareeb, Azza S; Younis, Mohamed S; El Fakahany, Amany F; Nagaty, Ibrahim M; Nagib, Marwa M

    2015-01-01

    Background: Many laboratories currently diagnose Blastocystis spp. infections by looking for the presence of vacuolar forms in faeces and the amoeboid form in diarrheal stools. Objectives: To investigate the best direct method in diagnosis of Blastocystis spp. and to study different morphological forms of the parasite. Materials and Methods: The study was carried out on one thousand and two hundred diarrheic stool samples. All samples were examined using direct smear, iodine stained smear, fo...

  2. OCCURRENCE OF Blastocystis spp. IN UBERABA, MINAS GERAIS, BRAZIL

    Science.gov (United States)

    CABRINE-SANTOS, Marlene; CINTRA, Eduardo do Nascimento; do CARMO, Rafaela Andrade; NASCENTES, Gabriel Antônio Nogueira; PEDROSA, André Luiz; CORREIA, Dalmo; de OLIVEIRA-SILVA, Márcia Benedita

    2015-01-01

    Intestinal parasites are a problem for public health all over the world. The infection with Blastocystis, a protozoan of controversial pathogenicity, is one of the most common among them all. In this study, the occurrence of intestinal parasites, with emphasis on Blastocystis, in patients at the Universidade Federal do Triângulo Mineiro was investigated in Uberaba (MG) through microscopy of direct smears and fecal concentrates using Ritchie’s method. Feces of 1,323 patients were examined from April 2011 to May 2012. In 28.7% of them at least one intestinal parasite was identified, and the most frequent organisms were Blastocystis spp. (17.8%) and Giardia intestinalis (7.4%). The occurrence of parasitism was higher in children aged 6 -10 years old, and the infection with Blastocystis spp. was higher above the age of six (p < 0.001). The exclusive presence of G. intestinalis and of Blastocystis spp. was observed in 5.4% and 12.2% of the patients, respectively. Regarding patients with diarrheic feces, 8% revealed unique parasitism of Blastocystis spp. Other intestinal parasites observed in children were Ascaris lumbricoides(0.3%) and Entamoeba histolytica/dispar/moshkovskii (1.4%). The Ritchie’s method was more sensitive (92.8%) when compared to direct microscopy (89.8%), with high agreement between them (97.7%, kappa = 0.92). In conclusion, the occurrence of Blastocystis spp. in Uberaba is high and the presence of diarrheic feces with exclusive presence of the parasite of Blastocystis spp. was observed. PMID:26200960

  3. Nutritional evaluation of hominy as poultry feed

    Directory of Open Access Journals (Sweden)

    I Putu Kompiang

    1998-10-01

    Full Text Available A series of chemical, biological assay and growth trials were conducted to determine the nutritive value of hominy, relative to corn. The crude protein, ash, NDF and ADF content of hominy were 10.25%, 2.10%, 19.10% and 4.70% respectively, and are higher than the corn level, 8.5%, 0.40%, 2.30% and 1.90% respectively. While its crude fat (3.20% and metabolize energy (3,020 kcal/kg contents were lower than the corn level 4,40% and 330 kcal/kg. Seven hundred DOC broilers of commercial strain were used for growth trial for 42 days. The birds were divided into 5 treatments group with 4 replications (35 bird/replicate. Experimental rations, starter and finisher, were formulated with graded levels of hominy on the expense of corn (A = 0%, B = 33.3%, C = 50%, D= 66.6% and E = 100% with similar level of crude protein and metabolize energy. Feed consumption was not affected (P>0.05 by the treatments, however body weight gain (BWG and feed conversion ratio (FCR were both significantly (P<0.05 affected by the treatments. Body weight gain of treatment A (1,712 g was similar to treatment B (1,683 g and C (1,682 g but significantly (P<0.05 higher than treatment D (1,598 g and E (1,570 g. Feed convertion ratio of treatment A (2.17 was similar to the treatment B (2.26 but significantly better (P<0.05 than treatment C (2.30, D (2.36 and E (2.39. Carcass quality was not affected by the treatments except for the shank color, where treatment A (7.38 was significantly yellower than the other treatments (B=2.50, C=2.75, D=1.75 and E=1.0. It is concluded that the protein content of hominy is higher than corn, but its metabolize energy was lower than the corn. Hominy could be included in the ration replacing corn up to 33.3% without affecting performance of the bird.

  4. The Mycoplasma hominis vaa gene displays a mosaic gene structure

    DEFF Research Database (Denmark)

    Boesen, Thomas; Emmersen, Jeppe M. G.; Jensen, Lise T.;

    1998-01-01

    Mycoplasma hominis contains a variable adherence-associated (vaa) gene. To classify variants of the vaa genes, we examined 42 M. hominis isolated by PCR, DNA sequencing and immunoblotting. This uncovered the existence of five gene categories. Comparison of the gene types revealed a modular...

  5. ОБОСНОВАНИЕ ВЗАИМОСВЯЗИ АНТИЛИЗОЦИМНОЙ АКТИВНОСТИ И РЕПРОДУКТИВНОЙ ФУНКЦИИ ЭНТЕРОКОККОВ ПРИ СОКУЛЬТИВИРОВАНИИ С ПРОСТЕЙШИМИ BLASTOCYSTIS HOMINIS

    OpenAIRE

    Бугеро, Н.

    2012-01-01

    В настоящей работе представлены результаты изучения персистентного потенциала у штаммов энтерококков, выделенных при дисбиозе кишечника, и изучена взаимосвязь антилизоцимной активности и репродуктивной функции энтерококков при сокультивировании с простейшими Blastocystis hominis. Полученные результаты позволяют говорить, что продуцирование микроорганизмами антилизоцимного фактора является, скорее, прерогативой стационарных, нежели активно делящихся бактериальных клеток. Установлена зависимост...

  6. Genetic diversity of human blastocystis isolates in khorramabad, central iran.

    Directory of Open Access Journals (Sweden)

    Ebrahim Badparva

    2014-03-01

    Full Text Available There are some genetic differences in Blastocystis that show the existence of species or genotypes. One of these genes that help in identifying Blastocystis is SSUrRNA. The aim of this study was assessment of genetic diversity of Blastocystis by PCR with seven pairs of STS primers.This study was done on 511 stool samples collected from patients referred to the health care centers of Khorramabad, Central Iran, in 2012. Genomic DNA was extracted and in order to determine the Blastocystis subtype in contaminated samples, seven pairs of primers STS (subtype specific sequence-tagged site were used.Out of 511 samples, 33 (6.5% samples were infected with Blastocystis. Subtype (ST of 30 samples was identified and three subtypes 2, 3 and 4 were determined. Mix infection was reported 10% which 3.33% of the infection was for the mixture of ST 3 and ST5 and 6.67% was for the mixture of ST 2 and ST 3.The predominant subtype was ST3 that is the main human subtype. The dominance of ST2 and 5 are important in this study. This superiority has been reported in some of the studies in ST 2 which is different from the studies in other countries, because they have announced priorities of the ST1 and ST6 after ST3.

  7. Geographic distribution of human Blastocystis subtypes in South America.

    Science.gov (United States)

    Ramírez, Juan David; Sánchez, Angie; Hernández, Carolina; Flórez, Carolina; Bernal, María Consuelo; Giraldo, Julio Cesar; Reyes, Patricia; López, Myriam Consuelo; García, Lineth; Cooper, Philip J; Vicuña, Yosselin; Mongi, Florencia; Casero, Rodolfo D

    2016-07-01

    Blastocystis is a cosmopolitan enteric protist colonizing probably more than 1 billion people. This protozoan exhibits genetic diversity and is subdivided into subtypes (STs). The aim of this study was to determine the distribution of Blastocystis STs in symptomatic and asymptomatic human samples from different countries of South America. A total of 346 fecal samples were genotyped by SSU rDNA showing ST1 (28.3%), ST2 (22.2%), ST3 (36.7%), ST4 (2%), ST5 (2.3%), ST6 (2%), ST7 (2.3%), ST8 (0.6%), ST12 (0.9%) and a novel ST (2.7%). These findings update the epidemiology of Blastocystis in South America and expand our knowledge of the phylogeographic differences exhibited by this stramenopile. PMID:27034056

  8. 人芽囊原虫标本染色方法的改进%Staining Improvement for Blastocystis hominis Specimen

    Institute of Scientific and Technical Information of China (English)

    张瑞其; 陈厚彪; 危会萍; 苏水莲

    2008-01-01

    人芽囊原虫标本经肖氏液固定后,用改良的哈氏苏木素染色.结果 表明,标本内部结构清晰,中心体明显,与传统的铁苏木素染色方法相比,染色效果好,制片时间短,操作步骤简单.

  9. Morphological and Ultrastructural Observation of Blastocystis hominis%人芽囊原虫的形态与超微结构

    Institute of Scientific and Technical Information of China (English)

    何妮; 张月清; 洪明理; 丛敏

    2001-01-01

    目的观察人芽囊原虫的形态与超微结构.方法对人芽囊原虫培养4~5 d的培养物进行多种染色后置光镜下观察形态结构,同时经过4%的戊二醛固定处理后在透射电镜下观察超微结构.结果人芽囊原虫形态有空泡型、颗粒型、阿米巴型、复分裂型及包囊型等.分裂方式有二分裂方式及孢子分裂方式.透射电镜下可见虫体内含有细胞核、线粒体、粗面内质网、脂滴和溶酶体等细胞器,泡状结构内含有糖原颗粒.结论人芽囊原虫空泡型的泡状结构可能与储留排泄物有关,阿米巴型可能系该虫的致病类型.

  10. Genomic and gene variation in Mycoplasma hominis strains

    DEFF Research Database (Denmark)

    Christiansen, Gunna; Andersen, H; Birkelund, Svend;

    1987-01-01

    DNAs from 14 strains of Mycoplasma hominis isolated from various habitats, including strain PG21, were analyzed for genomic heterogeneity. DNA-DNA filter hybridization values were from 51 to 91%. Restriction endonuclease digestion patterns, analyzed by agarose gel electrophoresis, revealed no...... identity or cluster formation between strains. Variation within M. hominis rRNA genes was analyzed by Southern hybridization of EcoRI-cleaved DNA hybridized with a cloned fragment of the rRNA gene from the mycoplasma strain PG50. Five of the M. hominis strains showed identical hybridization patterns. These...... hybridization patterns were compared with those of 12 other mycoplasma species, which showed a much more complex band pattern. Cloned nonribosomal RNA gene fragments of M. hominis PG21 DNA were analyzed, and the fragments were used to demonstrate heterogeneity among the strains. A monoclonal antibody against...

  11. Organelles in Blastocystis that Blur the Distinction between Mitochondria and Hydrogenosomes

    OpenAIRE

    Stechmann, Alexandra; Hamblin, Karleigh; Pérez-Brocal, Vicente; Gaston, Daniel; Richmond, Gregory S.; van der Giezen, Mark; Clark, C. Graham; Andrew J Roger

    2008-01-01

    Summary Blastocystis is a unicellular stramenopile of controversial pathogenicity in humans [1, 2]. Although it is a strict anaerobe, Blastocystis has mitochondrion-like organelles with cristae, a transmembrane potential and DNA [2–4]. An apparent lack of several typical mitochondrial pathways has led some to suggest that these organelles might be hydrogenosomes, anaerobic organelles related to mitochondria [5, 6]. We generated 12,767 expressed sequence tags (ESTs) from Blastocystis and ident...

  12. Molecular characterization of Blastocystis isolates from zoo animals and their animal-keepers.

    Science.gov (United States)

    Parkar, Unaiza; Traub, Rebecca J; Vitali, Simone; Elliot, Aileen; Levecke, Bruno; Robertson, Ian; Geurden, Thomas; Steele, Jan; Drake, Bev; Thompson, R C Andrew

    2010-04-19

    Blastocystis is an enteric protist and one of the most frequently reported parasitic infections in humans and a variety of animal hosts. It has also been reported in numerous parasite surveys of animals in zoological gardens and in particular in non-human primate species. PCR-based methods capable of the direct detection of Blastocystis in faeces were used to detect Blastocystis from various hosts, including non-human primates, Australian native fauna, elephants and giraffes, as well as their keepers from a Western Australian zoo. Additional faecal samples were also collected from elephants and giraffes from four other zoos in Amsterdam (The Netherlands), Antwerp (Belgium), Melbourne and Werribee (Australia). Information regarding the general health and lifestyle of the human volunteers were obtained by questionnaire. Overall, 42% and 63% of animals and zoo-keepers sampled from the Western Australian zoo were positive for Blastocystis, respectively. The occurrence of Blastocystis in elephants and giraffes from other cities was similar. This is the first report of Blastocystis found in the elephant, giraffe, quokka, southern hairy nosed wombat and western grey kangaroo. Three novel and what appear to be highly host-specific subtypes (STs) of Blastocystis in the elephant, giraffe and quokka are also described. These findings indicate that further exploration of the genetic diversity of Blastocystis is crucial. Most zoo-keepers at the Perth Zoo were harbouring Blastocystis. Four of these zoo-keeper isolates were identical to the isolates from the southern hairy nosed wombat and five primate species.

  13. Common occurrence of Cryptosporidium hominis in horses and donkeys.

    Science.gov (United States)

    Jian, Fuchun; Liu, Aiqin; Wang, Rongjun; Zhang, Sumei; Qi, Meng; Zhao, Wei; Shi, Yadong; Wang, Jianling; Wei, Jiujian; Zhang, Longxian; Xiao, Lihua

    2016-09-01

    Extensive genetic variation is observed within the genus Cryptosporidium and the distribution of Cryptosporidium species/genotypes in humans and animals appears to vary by geography and host species. To better understand the genetic diversity of Cryptosporidium spp. in horses and donkeys, we characterized five horse-derived and 82 donkey-derived Cryptosporidium isolates from five provinces or autonomous regions (Sichuan, Gansu, Henan, Inner Mongolia and Shandong) in China at the species/genotype and subtype levels. Three Cryptosporidium species/genotypes were identified based on the analysis of the SSU rRNA gene, including Cryptosporidium parvum (n=22), the Cryptosporidium horse genotype (n=4), and Cryptosporidium hominis (n=61). The identification of C. hominis was confirmed by sequence analysis of the HSP70 and actin genes. Subtyping using sequence analysis of the 60kDa glycoprotein gene identified 21 C. parvum isolates as subtype IIdA19G1, the four horse genotype isolates as subtypes VIaA15G4 (n=2) and VIaA11G3 (n=2), and the 61 C. hominis isolates as IkA16G1 (n=59) and IkA16 (n=2). The common finding of C. hominis reaffirms the heterogeneity of Cryptosporidium spp. in horses and donkeys and is possibly a reflection of endemic transmission of C. hominis in these animals. Data of the study suggest that horses and donkeys as companion animals may potentially transmit Cryptosporidium infections to humans. PMID:27264727

  14. Serological differentiation of microsporidia with special reference to Trachipleistophora hominis

    Directory of Open Access Journals (Sweden)

    Cheney S.A.

    2001-06-01

    Full Text Available Myositis is a common clinical syndrome in advanced stages of AIDS. Trachipleistophora hominis (phylum Microspora has been detected in several cases of painful, immobilising myositis in AIDS patients. Enzyme linked immunosorbent assays (ELISAs and Western blotting of protein profiles separated by SDS PAGE were used to determine whether this species could be detected and differentiated by serology. Sixteen microsporidia, including several species known to infect man and species infecting fish, crustaceans and a mosquito, were used as antigen. Each species had a unique profile of SDS PAGE-separated proteins. In Western blots, mouse antiserum, raised to T. hominis and selected for its high ELISA specificity, bound to antigens ranging from less than 25 kDa to greater than 250 kDa with major bands at 39-44 kDa and 98-150 kDa on T. hominis protein profiles. The serum also recognised some high molecular weight antigens in the profiles of Vavraia culicis, Heterosporis anguillarum, and three species of Pleistophora but none in the remaining genera examined. It was concluded that ELISA and Western blotting could be used to detect and differentiate T. hominis in muscle biopsy tissue from patients with myositis. However, sera from T. hominis infected patients in the terminal stages of AIDS would not be useful for detection of infections because of a sharp decline in antibody level.

  15. A retrospective metagenomics approach to studying Blastocystis

    DEFF Research Database (Denmark)

    Andersen, Lee O'Brien; Bonde, Ida; Nielsen, Henrik Bjørn;

    2015-01-01

    a selection of 316 human faecal samples, hence representing genes originating from a single subtype. The 316 faecal samples were from 236 healthy individuals, 13 patients with Crohn's disease (CD) and 67 patients with ulcerative colitis (UC). The prevalence of Blastocystis was 20.3% in the healthy individuals...... metagenomic datasets targeting the bacterial component of the intestinal microbiome and the interplay between these microbial communities....

  16. Cardiobacterium hominis endocarditis: two cases and a review of the literature

    OpenAIRE

    Malani, A. N.; Aronoff, D. M.; Bradley, S. F.; Kauffman, C A

    2006-01-01

    Cardiobacterium hominis, a member of the HACEK group (Haemophilus parainfluenzae, Haemophilus aphrophilus, and Haemophilus paraphrophilus, Actinobacillus actinomycetemcomitans, C. hominis, Eikenella corrodens, and Kingella species), is a rare cause of endocarditis. There are 61 reported cases of C. hominis infective endocarditis in the English-language literature, 15 of which involved prosthetic valve endocarditis. There is one reported case of C. hominis after upper endoscopy and none report...

  17. Mycoplasma hominis Meningitis in a 24 Week Premature Neonate: Case Report and Short Literature Review

    OpenAIRE

    Watson, Louise; Pang, Yee Min; Mitchell, Simon; Dodgson, Andrew

    2008-01-01

    Over the last 20-year period there have been fewer than 10 reported cases of Mycoplasma hominis central nervous system infection in either premature or full term infants. The optimum management of M hominis infection in premature infants is still unclear. We report the case of a premature infant with persistent central nervous system infection caused by M hominis treated successfully with intravenous chloramphenicol. Previous reports of M hominis central nervous infection and its management a...

  18. Blastocystis sp. and other intestinal parasites in hemodialysis patients

    Directory of Open Access Journals (Sweden)

    Rose Anne Kulik

    2008-08-01

    Full Text Available Chronic renal insufficiency disease (CRI leads to uremia in hemodialysis patients and induces a state of immunodepression that results in higher frequencies of infections and diarrhea. Hemodialysis patients resident in the city of Campo Mourão, Paraná, Brazil were analyzed from April 2006 through September 2007 for Blastocystis sp. and other intestinal parasites and for associated diarrhea. Fecal samples from 86 hemodialysis patients and 146 healthy (reference persons were examined by standard methods for detecting ova, larvae and cysts, which included preservation in 10% formalin and the Kinyoun method. Thirty-three hemodialysis patients (45.1% and 36 reference individuals (25.7% were found to be parasitized. The differences in the percentages of parasitism and polyparasitism between the reference group and the chronic renal patients was significant (p= 0.0318 and 0.0019, respectively. Blastocystis sp. (18%-20.1%, Endolimax nana (14%-16.3%, Cryptosporidium sp. (4%-4.7% and Entamoeba coli (4%-4.7% were the most frequent protozoa found in the hemodialysis patients. Parasitism was not significantly associated with diarrhea (p=0.9947 or with decreased white blood cell counts (p=0.7046 in these individuals. Because parasitic infections may be an important comorbidity factor in hemodialysis patients, we suggest that parasitological stool examinations, especially for Blastocystis sp. and Cryptosporidium sp., be included in routine medical follow-up examinations of these patients.

  19. Campylobacter hominis sp nov., from the human gastrointestinal tract

    DEFF Research Database (Denmark)

    Lawson, A.J.; On, Stephen L.W.; Logan, J.M.J.;

    2001-01-01

    Sequences of 16S rDNA of a novel campylobacter from faeces of healthy humans were previously shown to originate from a new taxon, 'Candidatus Campylobacter hominis', which could not be cultured. Since phylogenetic analysis suggested that anaerobic conditions might be required for growth, an isola......Sequences of 16S rDNA of a novel campylobacter from faeces of healthy humans were previously shown to originate from a new taxon, 'Candidatus Campylobacter hominis', which could not be cultured. Since phylogenetic analysis suggested that anaerobic conditions might be required for growth......, an isolation strategy was developed employing initial non-selective membrane filtration onto fastidious anaerobe agar. Campylobacters were then isolated from the resulting mixed microbial flora by a dilution strategy and/or by immunomagnetic separation with genus-specific polyclonal antibody. Isolates were...... phenotypic characteristics. The name Campylobacter hominis sp. nov. is proposed for the new species, the type strain of which is NCTC 13146(T)(= LMG 19568(T))....

  20. Acute Blastocystis-Associated Appendicular Peritonitis in a Child, Casablanca, Morocco

    OpenAIRE

    Fréalle, Emilie; El Safadi, Dima; Cian, Amandine; Aubry, Estelle; Certad, Gabriela; Osman, Marwan; Wacrenier, Agnès; Dutoit, Emmanuel; Creusy, Colette; Dubos, François; Viscogliosi, Eric

    2015-01-01

    Despite increasing reports that Blastocystis infection is associated with digestive symptoms, its pathogenicity remains controversial. We report appendicular peritonitis in a 9-year-old girl returning to France from Morocco. Only Blastocystis parasites were detected in stools, appendix, peritoneal liquid, and recto-uterine pouch. Simultaneous gastroenteritis in 26 members of the child’s family suggested an outbreak.

  1. Molecular Characterization and Subtyping of Blastocystis Species in Irritable Bowel Syndrome Patients from North India.

    Science.gov (United States)

    Das, Rojaleen; Khalil, Shehla; Mirdha, B R; Makharia, Govind K; Dattagupta, Siddharta; Chaudhry, Rama

    2016-01-01

    Blastocystis species has been extensively studied in recent few years to establish its pathogenecity. Present study was designed to identify and examine the association of Blastocystis sp. and its subtypes with Irritable Bowel Syndrome (IBS).Blastocystis sp. detected using wet-mount microscopy, trichrome staining, in-vitro culture and Polymerase Chain Reaction (PCR) assay in a cohort of IBS patients (n = 150) and healthy controls (n = 100). Isolates of Blastocystis sp.were subtyped using Sequence Tagged Site and representative samples were sequenced at SSUrRNA locus.A total of sixty five isolates of Blastocystis sp. were identified [IBS (n = 50); Controls (n = 15)] of which 91% belonged to ST3 and 9% belonged to ST1. No other subtypes could be identified. Statistically significant association was observed between Blastocystis sp. and IBS patients; however no particular subtype could be ascertained to any particular clinical type of IBS.The frequency of occurrence of Blastocystis sp. was more in IBS patients as compared to the controls and ST3 being the most prevalent subtype. The genetic polymorphism of SSU-rRNA gene amongst the different Blastocystis sp.isolates found in this study reinforces the fact that these organisms are genetically highly divergent. PMID:26784888

  2. Draft genome sequence of the intestinal parasite Blastocystis subtype 4-isolate WR1.

    Science.gov (United States)

    Wawrzyniak, Ivan; Courtine, Damien; Osman, Marwan; Hubans-Pierlot, Christine; Cian, Amandine; Nourrisson, Céline; Chabe, Magali; Poirier, Philippe; Bart, Aldert; Polonais, Valérie; Delgado-Viscogliosi, Pilar; El Alaoui, Hicham; Belkorchia, Abdel; van Gool, Tom; Tan, Kevin S W; Ferreira, Stéphanie; Viscogliosi, Eric; Delbac, Frédéric

    2015-06-01

    The intestinal protistan parasite Blastocystis is characterized by an extensive genetic variability with 17 subtypes (ST1-ST17) described to date. Only the whole genome of a human ST7 isolate was previously sequenced. Here we report the draft genome sequence of Blastocystis ST4-WR1 isolated from a laboratory rodent at Singapore. PMID:26484170

  3. Draft genome sequence of the intestinal parasite Blastocystis subtype 4-isolate WR1

    Directory of Open Access Journals (Sweden)

    Ivan Wawrzyniak

    2015-06-01

    Full Text Available The intestinal protistan parasite Blastocystis is characterized by an extensive genetic variability with 17 subtypes (ST1–ST17 described to date. Only the whole genome of a human ST7 isolate was previously sequenced. Here we report the draft genome sequence of Blastocystis ST4-WR1 isolated from a laboratory rodent at Singapore.

  4. GENETIC SUBTYPES OF BLASTOCYSTIS ISOLATED FROM THAI HOSPITALIZED PATIENTS IN NORTHEASTERN THAILAND.

    Science.gov (United States)

    Sanpool, Oranuch; Laoraksawong, Pokkamol; Janwan, Penchom; Intapan, Pewpan M; Sawanyawisuth, Kittisak; Thanchomnang, Tongjit; Changtrakul, Yossombat; Maleewong, Wanchai

    2015-03-01

    Blastocystis sp is probably the most common intestinal protozoan of humans. This taxon is known to include more than 17 subtypes, some of which likely cause human disease. We investigated the distribution of Blastocystis subtypes in Thai patients admitted for a variety of conditions at a hospital in northeastern Thailand. Fresh fecal samples, positive for Blastocystis by microscopy, were individually cultured in Jones' medium (n = 20) and each sample was used for amplification and sequencing a fragment of 18S rDNA. BLAST search and phylogenetic analysis demonstrated that Blastocystis subtypes ST1 (20%), ST3 (60%), ST6 (10%) and ST7 (10%) were present. No clear link between gastro-intestinal symptoms and any particular subtype of Blastocystis was apparent. Thus, there is a need to extend the work to evaluate clinical signs and subtypes in a larger cohort of patients. PMID:26513920

  5. Characterization of two cysteine proteases secreted by Blastocystis ST7, a human intestinal parasite.

    Science.gov (United States)

    Wawrzyniak, Ivan; Texier, Catherine; Poirier, Philippe; Viscogliosi, Eric; Tan, Kevin S W; Delbac, Frédéric; El Alaoui, Hicham

    2012-09-01

    Blastocystis spp. are unicellular anaerobic intestinal parasites of both humans and animals and the most prevalent ones found in human stool samples. Their association with various gastrointestinal disorders raises the questions of its pathogenicity and of the molecular mechanisms involved. Since secreted proteases are well-known to be implicated in intestinal parasite virulence, we intended to determine whether Blastocystis spp. possess such pathogenic factors. In silico analysis of the Blastocystis subtype 7 (ST7) genome sequence highlighted 22 genes coding proteases which were predicted to be secreted. We characterized the proteolytic activities in the secretory products of Blastocystis ST7 using specific protease inhibitors. Two cysteine proteases, a cathepsin B and a legumain, were identified in the parasite culture supernatant by gelatin zymographic SDS-PAGE gel and MS/MS analysis. These proteases might act on intestinal cells and disturb gut function. This work provides serious molecular candidates to link Blastocystis spp. and intestinal disorders.

  6. Blastocystis Isolate B Exhibits Multiple Modes of Resistance against Antimicrobial Peptide LL-37.

    Science.gov (United States)

    Yason, John Anthony; Ajjampur, Sitara Swarna Rao; Tan, Kevin Shyong Wei

    2016-08-01

    Blastocystis is one of the most common eukaryotic organisms found in humans and many types of animals. Several reports have identified its role in gastrointestinal disorders, although its pathogenicity is yet to be clarified. Blastocystis is transmitted via the fecal-to-oral route and colonizes the large intestines. Epithelial cells lining the intestine secrete antimicrobial peptides (AMPs), including beta-defensins and cathelicidin, as a response to infection. This study explores the effects of host colonic antimicrobial peptides, particularly LL-37, a fragment of cathelicidin, on different Blastocystis subtypes. Blastocystis is composed of several subtypes that have genetic, metabolic, and biological differences. These subtypes also have various outcomes in terms of drug treatment and immune response. In this study, Blastocystis isolates from three different subtypes were found to induce intestinal epithelial cells to secrete LL-37. We also show that among the antimicrobial peptides tested, only LL-37 has broad activity on all the subtypes. LL-37 causes membrane disruption and causes Blastocystis to change shape. Blastocystis subtype 7 (ST7), however, showed relative resistance to LL-37. An isolate, ST7 isolate B (ST7-B), from this subtype releases proteases that can degrade the peptide. It also makes the environment acidic, which causes attenuation of LL-37 activity. The Blastocystis ST7-B isolate was also observed to have a thicker surface coat, which may protect the parasite from direct killing by LL-37. This study determined the effects of LL-37 on different Blastocystis isolates and indicates that AMPs have significant roles in Blastocystis infections. PMID:27217421

  7. A sebaceous cyst with a difference: Dermatobia hominis

    OpenAIRE

    Harbin, L J; Khan, M.; Thompson, E M; Goldin, R D

    2002-01-01

    Dermatobia hominis causes furuncular myiasis and is endemic to South America. This report describes a case in a young woman who had recently visited Belize, highlighting the importance of clinical history (including travel history) and close liaison between pathologist and surgeon.

  8. Complete Genome Sequence of the Human Gut Symbiont Roseburia hominis

    DEFF Research Database (Denmark)

    Travis, Anthony J.; Kelly, Denise; Flint, Harry J;

    2015-01-01

    We report here the complete genome sequence of the human gut symbiont Roseburia hominis A2-183(T) (= DSM 16839(T) = NCIMB 14029(T)), isolated from human feces. The genome is represented by a 3,592,125-bp chromosome with 3,405 coding sequences. A number of potential functions contributing to host...

  9. Antigenic and genomic homogeneity of successive Mycoplasma hominis isolates

    DEFF Research Database (Denmark)

    Jensen, L T; Thorsen, P; Møller, B;

    1998-01-01

    Sixty Mycoplasma hominis isolates were obtained from the cervices of pregnant women and from the ears or pharynges of their newborn babies. The isolates were examined by SDS-PAGE and pulsed-field gel electrophoresis. Antigenic and genomic profiles were obtained for 16 series with two or more...

  10. Analysis of a Mycoplasma hominis membrane protein, P120

    DEFF Research Database (Denmark)

    Christiansen, Gunna; Mathiesen, SL; Nyvold, Charlotte Guldborg;

    1994-01-01

    The monoclonal antibody mAb 26.7D generated against a clinical isolate of Mycoplasma hominis 7488 was shown to react with a surface-exposed epitope on a 120-kDa protein (P120). The gene encoding the protein was cloned and sequenced, and the transcriptional start point was determined by primer...

  11. DETECTION OF MYCOPLASMA HOMINIS IN PATIENTS WITH URINARY TRACT INFECTIONS

    Directory of Open Access Journals (Sweden)

    Osama M.S. Abdul-Wahab

    2014-01-01

    Full Text Available Mycoplasma hominis is one of the well known genital mycoplasma agent that has been recognized as potentially pathogenic species and associated with various infectious diseases in adult men and woman. The organism could cause pelvic inflammatory disease, postpartum, postabortion fever and pyelonephritis. The Aim of this prospective study was to detemine the occurrence and prevalence of Mycoplasma hominis in patients with urinary tract infections, since they are usually not detected by routine microbiological examination of urine samples in laboratory department. For this purpose, Culture method was to be attempted as the current laboratory methods of choice for detection urogenital mycoplasmas infections. This study was designed as a case-control study in which a total of 100 patients with mean age of the of 42.78±22.49 (95% CI = 39-49.17 in case group as well as a mean age of 48.18±18.81 ((95% CI = 42.83-53.53 in the control group. The patients of both sexes were investigated for the presence of M. hominis and they were admitted to the urology department at asser central hospital in Abha (a city in southwest region of Saudi Arabia, during over a period of one year (February, 2013-March, 2014. A detailed history with specific urinary symptoms and signs was obtained as a result. These patients were divided into two groups: The first group (or case group and the second group (or control group: The case group, included 50 symptomatic patients who had been admitted in hospital and they were examined for the presence of one or more of the following specific urinary symptoms and signs: Urinary frequency, dysuria, suprapubic/pelvic pain. Unlike the case group, the control group included 50 asymptomatic patients who were free of any specific urinary symptoms and they were matched by the first group for their age index. First voided urine specimens were tested using urine microscopy, culture for M hominis was performed and

  12. Blastocystis sp. in Irritable Bowel Syndrome (IBS)--Detection in Stool Aspirates during Colonoscopy.

    Science.gov (United States)

    Ragavan, Nanthiney Devi; Kumar, Suresh; Chye, Tan Tian; Mahadeva, Sanjiv; Shiaw-Hooi, Ho

    2015-01-01

    Blastocystis is one of the most common gut parasites found in the intestinal tract of humans and animals. Its' association with IBS is controversial, possibly as a result of irregular shedding of parasites in stool and variation in stool detection. We aimed to screen for Blastocystis in colonic stool aspirate samples in adult patients with and without IBS undergoing colonoscopy for various indications and measure the interleukin levels (IL-8, IL-3 and IL-5). In addition to standard stool culture techniques, polymerase chain reaction (PCR) techniques were employed to detect and subtype Blastocystis. All the serum samples collected were subjected for ELISA studies to measure the interleukin levels (IL-8, IL-3 and IL-5). Among 109 (IBS n = 35 and non-IBS n = 74) adults, direct stool examination and culture of colonic aspirates were initially negative for Blastocystis. However, PCR analysis detected Blastocystis in 6 (17%) IBS and 4 (5.5%) non-IBS patients. In the six positive IBS patients by PCR method, subtype 3 was shown to be the most predominant (3/6: 50%) followed by subtype 4 (2/6; 33.3%) and subtype 5 (1/6; 16.6%). IL-8 levels were significantly elevated in the IBS Blasto group and IBS group (pBlastocystis-infected stools. Patients with IBS infected with parasite showed an increase in the interleukin levels demonstrate that Blastocystis does have an effect in the immune system. PMID:26375823

  13. Colonization with the enteric protozoa Blastocystis is associated with increased diversity of human gut bacterial microbiota.

    Science.gov (United States)

    Audebert, Christophe; Even, Gaël; Cian, Amandine; Loywick, Alexandre; Merlin, Sophie; Viscogliosi, Eric; Chabé, Magali

    2016-01-01

    Alterations in the composition of commensal bacterial populations, a phenomenon known as dysbiosis, are linked to multiple gastrointestinal disorders, such as inflammatory bowel disease and irritable bowel syndrome, or to infections by diverse enteric pathogens. Blastocystis is one of the most common single-celled eukaryotes detected in human faecal samples. However, the clinical significance of this widespread colonization remains unclear, and its pathogenic potential is controversial. To address the issue of Blastocystis pathogenicity, we investigated the impact of colonization by this protist on the composition of the human gut microbiota. For that purpose, we conducted a cross-sectional study including 48 Blastocystis-colonized patients and 48 Blastocystis-free subjects and performed an Ion Torrent 16S rDNA gene sequencing to decipher the Blastocystis-associated gut microbiota. Here, we report a higher bacterial diversity in faecal microbiota of Blastocystis colonized patients, a higher abundance of Clostridia as well as a lower abundance of Enterobacteriaceae. Our results contribute to suggesting that Blastocystis colonization is usually associated with a healthy gut microbiota, rather than with gut dysbiosis generally observed in metabolic or infectious inflammatory diseases of the lower gastrointestinal tract. PMID:27147260

  14. Blastocystis sp. infection in patients with gastrointestinal complaints: a Cuban study

    Directory of Open Access Journals (Sweden)

    Roberto Cañete

    2014-12-01

    techniques, the fresh samples were examined under light microscope.[¤]RESULTS[|]Blastocystis sp. was detected in 49 patients (44.5% in the symptomatic group and 28 (22.4% in the asymptomatic one. Abdominal pain (52.7% or distension (38.2%, acute diarrhea (26.4%, loss of appetite (18.2%, and dyspepsia (16.4% were the gastrointestinal complains notified by patients included on the symptomatic group. The probability to identify Blastocystis sp. in patients with gastrointestinal complains was 2.9 times higher than in asymptomatic patients.[¤]CONCLUSION[|]Current results support those authors considering Blastocystis sp. as pathogen.[¤

  15. Blastocystis in Health and Disease: Are We Moving from a Clinical to a Public Health Perspective?

    Science.gov (United States)

    Andersen, Lee O'Brien; Stensvold, Christen Rune

    2016-03-01

    Blastocystis is a genus of common single-celled intestinal parasitic protists with an unsettled role in human health and disease. Being a stable component of intestinal microbiota, once established, the Blastocystis parasite appears more common in healthy individuals than in patients with infectious, functional, or inflammatory bowel disease. Recent data suggest that the parasite is associated with certain gut microbiota profiles and health indices. Convincing data and tools differentiating asymptomatic colonization from infection are yet to be demonstrated. Although the parasite may elicit disease under certain circumstances, the focus on Blastocystis may be shifting from a clinical to a public health perspective. PMID:26677249

  16. Subtype distribution of Blastocystis isolates from synanthropic and zoo animals and identification of a new subtype

    DEFF Research Database (Denmark)

    Stensvold, C. R.; Alfellani, M. A.; Nørskov-Lauritsen, S.;

    2009-01-01

    Blastocystis isolates from 56 Danish synanthropic and zoo animals, 62 primates primarily from United Kingdom (UK) collections and 16 UK primate handlers were subtyped by PCR, sequencing and phylogenetic analysis. A new subtype (ST) from primates and artiodactyls was identified and designated...... as Blastocystis sp. ST10. STs isolated from non-human primates (n = 70) included ST3 (33%), ST8 (21%). ST2 (16%), ST5 (13%), ST1 (10%), ST4 (4%) and ST10(3%). A high prevalence of ST8 was seen among primate handlers (25%). This ST is normally very rare in humans, suggesting that acquisition of Blastocystis ST8...

  17. Presence of an Active Efflux System in the Fluoroquinolones Resistance of Mycoplasma Hominis

    Institute of Scientific and Technical Information of China (English)

    姚艳冰; 吴移谋; 朱翠明; 曾铁兵; 曾焱华

    2003-01-01

    Objective: To investigate the possible presence of an active efflux system in resistance to fluoroqninolones in Mycoplasma hominis. Methods: The resistant strains of M. hominis were selected from one hundred and three clinical strains of M. homlnls by broth microdilution method. The ac-cumulation of ciprofloxacin in M. hominis and the in-fluence of carbonyl cyanide m-chlorophenyl- hydrazone (CCCP) and reserpine were measured by a fluores-cence method. Results: Two resistant strains and two susceptible strains of M. hominis were selected in vitro. The accu-mulation of ciprofloxacin for resistant strains is lower than that of susceptible strains. CCCP and reserpine had different influence on clinical strains of M.hominis. Reserpine could dramatically increase the accumulation of ciprofloxacin, however CCCP had a little effect on it. Conclusion: These results suggest that the pres-ence of an active efflux system implicated in the fluoroouinolones-resistant in M. hominis.

  18. Granular Formation during Apoptosis in Blastocystis sp. Exposed to Metronidazole (MTZ).

    Science.gov (United States)

    Dhurga, Devi Balkrishnan; Suresh, Kumar; Tan, Tian Chye

    2016-01-01

    The role and function of the granular life cycle stage in Blastocystis sp, remains uncertain despite suggestions being made that the granules are metabolic, reproductive and lipid in nature. This present study aims to understand granular formation by triggering apoptosis in Blastocystis sp. by treating them with metronidazole (MTZ). Blastocystis sp.cultures of 4 sub-types namely 1, 2, 3 and 5 when treated with 0.01 and 0.0001 mg/ml of metronidazole (MTZ) respectively showed many of the parasites to be both viable and apoptotic (VA). Treated subtype 3 isolates exhibited the highest number of granular forms i.e. 88% (pBlastocystis sp. infections. PMID:27471855

  19. Transcriptomic profiling of host-parasite interactions in the microsporidian Trachipleistophora hominis

    OpenAIRE

    Watson, Andrew K.; Tom A Williams; Williams, Bryony A P; Karen A. Moore; Hirt, Robert P.; Embley, T. Martin

    2015-01-01

    Background Trachipleistophora hominis was isolated from an HIV/AIDS patient and is a member of a highly successful group of obligate intracellular parasites. Methods Here we have investigated the evolution of the parasite and the interplay between host and parasite gene expression using transcriptomics of T. hominis-infected rabbit kidney cells. Results T. hominis has about 30 % more genes than small-genome microsporidians. Highly expressed genes include those involved in growth, replicatio...

  20. The effects of Hominis Placenta extract on Melanin synthesis of B16 melanoma cells

    OpenAIRE

    Hyung-Sik Seo

    2006-01-01

    Objective : This research was carried out for the development of medicine for vitiligo treatment and focused on the effect of Hominis Placenta extract on Melanin synthesis of B16 melanoma cells. Methods : Acitivity of tyrosinase playing a vital role in synthesis of Melanin and the quantity of Melanin, which is the final product in cultured B16 melanoma cells, effects of Hominis Placenta extract were measured. Results : The results indicated that Hominis Placenta extract increased both t...

  1. Molecular mechanism of fluoroquinolones resistance in Mycoplasma hominis clinical isolates

    OpenAIRE

    Meng Dong-Ya; Sun Chang-Jian; Yu Jing-Bo; Ma Jun; Xue Wen-Cheng

    2014-01-01

    To evaluate the molecular mechanism of fluoroquinolones resistance in Mycoplasma hominis (MH) clinical strains isolated from urogenital specimens. 15 MH clinical isolates with different phenotypes of resistance to fluoroquinolones antibiotics were screened for mutations in the quinolone resistance-determining regions (QRDRs) of DNA gyrase (gyrA and gyrB) and topoisomerase IV (parC and parE) in comparison with the reference strain PG21, which is susceptible to fluoroquinolones antibiotics. 15 ...

  2. High lipid storage in vacoular forms of subtype 6 blastocystis sp. in ostrich

    OpenAIRE

    Chandrasekaran, Hemalatha; Govind, Suresh Kumar; Panchadcharam, Chandrawathani; Bathmanaban, Premaalatha; Raman, Kalyani; Thergarajan, Gaythri

    2014-01-01

    Background Blastocystis sp., a widely prevalent intestinal protozoan parasite is found in a wide range of animals, including humans. The possibility of zoonotic transmission to human from birds especially ostriches led us to investigate on the cross infectivity of Blastocystis sp. isolated from the ostrich feces as well as the phenotypic and subtype characteristics. There is a need to investigate this especially with the rising number of ostrich farms due to the growing global ostrich industr...

  3. Epidemiologic Study of Blastocystis Infection in an Urban Community in the Philippines

    Directory of Open Access Journals (Sweden)

    Maria Luz B. Belleza

    2015-01-01

    Full Text Available Blastocystis has been considered as the most common intestinal parasite in humans and has an augmented impact on public health. However, the prevalence of this parasite in the Philippines has not been determined. To contribute to a better understanding of the epidemiology of this infection, a cross-sectional study aimed at providing the first documented data on the prevalence and correlates, sociodemographic factors, hygiene practices, source of water supply, and dog ownership, associated with Blastocystis infection was carried out in randomly selected communities at Pateros, Metro Manila. Fecal samples from respondents were collected and cultured in diphasic agar medium for 3–7 days and examined using light microscopy. Of the 1,271 respondents, 12.98% (95% CI: 11.13–14.83 were detected positive for Blastocystis. Among the correlates of Blastocystis infection, dog ownership was found significantly associated as confirmed by multivariate analysis. Therefore, this factor should be considered in information to create awareness about Blastocystis and to prevent and control Blastocystis infection in particular and diarrheal diseases in general. Further studies using molecular approaches to distinguish subtype and to determine genetic characteristics of isolates from humans and dogs are recommended to analyze their relationship and provide more conclusive evidence of cross-transmission.

  4. Epidemiologic Study of Blastocystis Infection in an Urban Community in the Philippines.

    Science.gov (United States)

    Belleza, Maria Luz B; Cadacio, Jessa Louise C; Borja, Maridel P; Solon, Juan Antonio A; Padilla, Mildred A; Tongol-Rivera, Pilarita N; Rivera, Windell L

    2015-01-01

    Blastocystis has been considered as the most common intestinal parasite in humans and has an augmented impact on public health. However, the prevalence of this parasite in the Philippines has not been determined. To contribute to a better understanding of the epidemiology of this infection, a cross-sectional study aimed at providing the first documented data on the prevalence and correlates, sociodemographic factors, hygiene practices, source of water supply, and dog ownership, associated with Blastocystis infection was carried out in randomly selected communities at Pateros, Metro Manila. Fecal samples from respondents were collected and cultured in diphasic agar medium for 3-7 days and examined using light microscopy. Of the 1,271 respondents, 12.98% (95% CI: 11.13-14.83) were detected positive for Blastocystis. Among the correlates of Blastocystis infection, dog ownership was found significantly associated as confirmed by multivariate analysis. Therefore, this factor should be considered in information to create awareness about Blastocystis and to prevent and control Blastocystis infection in particular and diarrheal diseases in general. Further studies using molecular approaches to distinguish subtype and to determine genetic characteristics of isolates from humans and dogs are recommended to analyze their relationship and provide more conclusive evidence of cross-transmission. PMID:26074981

  5. Fluorogenic assay for differentiating Staphylococcus warneri and Staphylococcus hominis strains of bovine origin.

    OpenAIRE

    White, D. G.; Harmon, R J; Langlois, B E

    1990-01-01

    A fluorogenic assay for the detection of beta-glucosidase was developed as part of a simplified conventional method to distinguish Staphylococcus warneri and Staphylococcus hominis isolated from bovine body sites. The assay is based on the fact that strains of S. warneri produce beta-glucosidase, while strains of S. hominis do not.

  6. Prevalence and Antibiotic Susceptibility of Mycoplasma hominis and Ureaplasma urealyticum in Pregnant Women.

    Science.gov (United States)

    Lee, Min Young; Kim, Myeong Hee; Lee, Woo In; Kang, So Young; Jeon, You La

    2016-09-01

    Mycoplasma hominis (M. hominis) and Ureaplasma urealyticum (U. urealyticum) are important opportunistic pathogens that cause urogenital infections and complicate pregnancy. The aim of this study was to investigate the prevalence, effects on pregnancy outcomes, and antimicrobial susceptibilities of M. hominis and U. urealyticum. We tested vaginal swabs obtained from 1035 pregnant women for the presence of genital mycoplasmas between June 2009 and May 2014. The laboratory and clinical aspects of genital mycoplasmas infection were reviewed retrospectively, and the identification and antimicrobial susceptibility of genital mycoplasmas were determined using the Mycoplasma IST-2 kit. A total of 571 instances of M. hominis and/or U. urealyticum were detected. Of them, M. hominis was detected in two specimens, whereas U. urealyticum was detected in 472 specimens. The remaining 97 specimens were positive for both M. hominis and U. urealyticum. Preterm deliveries were frequently observed in cases of mixed infection of M. hominis and U. urealyticum, and instances of preterm premature rupture of membrane were often found in cases of U. urealyticum. The rates of non-susceptible isolates to erythromycin, empirical agents for pregnant women, showed increasing trends. In conclusion, the prevalence of M. hominis and/or U. urealyticum infections in pregnant women is high, and the resistance rate of antimicrobial agents tends to increase. Therefore, to maintain a safe pregnancy, it is important to identify the isolates and use appropriate empirical antibiotics immediately. PMID:27401661

  7. An epidemiological survey of Mycoplasma hominis and Ureaplasma urealyticum in gynaecological outpatients, Rome, Italy.

    Science.gov (United States)

    Verteramo, R; Patella, A; Calzolari, E; Recine, N; Marcone, V; Osborn, J; Chiarini, F; Degener, A M

    2013-12-01

    The objective of this study was to assess the prevalence of Ureaplasma urealyticum and Mycoplasma hominis infections and to investigate associations between their presence in the lower female genital tract and lifestyle characteristics. The study was performed on a population of 3115 women, comparing the demographic and behavioural characteristics of 872 women with U. urealyticum infection and 142 women with M. hominis with uninfected women, using univariate and multiple logistic regression analysis. The prevalence of infection with U. urealyticum was 28% and M. hominis was 4.6%. In multivariate logistic regression analysis, intrauterine device, number of sexual partners and age (<35 years) were significantly associated with U. urealyticum while previous induced abortion, condom use and young age at first intercourse (<16 years) were associated with M. hominis infection. U. urealyticum infection presents the same demographic and behavioural characteristics of a sexually transmitted disease. The unprotective role of condom use suggests a non-sexual mode of transmission of M. hominis infection.

  8. Subtype Distribution of Blastocystis in Communities along the Chao Phraya River, Thailand.

    Science.gov (United States)

    Palasuwan, Attakorn; Palasuwan, Duangdao; Mahittikorn, Aongart; Chiabchalard, Rachatawan; Combes, Valery; Popruk, Supaluk

    2016-08-01

    Blastocystis is a common zoonotic enteric protozoan that has been classified into 17 distinct subtypes (STs). A cross-sectional study was conducted to determine the prevalence and subtype distributions of Blastocystis in villagers living along the Chao Phraya River, Ayutthaya Province, Thailand, and to assess the risk of zoonotic infection. In total, 220 stool samples were collected, and DNA was extracted. PCR and sequencing were performed with primers targeting the small-subunit ribosomal RNA (SSU rRNA) genes. Blastocystis was present in 5.9% (13/220) of samples, and ST3 (5.0%; 11/220) was the predominant subtype, followed by ST2 (0.45%; 1/220) and ST6 (0.45%; 1/220). Phylogenetic trees were constructed with the maximum-likelihood method based on the Hasegawa-Kishino-Yano + G + I model, neighbor-joining, and maximum parsimony methods. The percentage of bootstrapped trees in which the associated taxa clustered together was relatively high. All the sequences of the Blastocystis-positive samples (KU051524-KU051536) were closely related to those from animals (pig, cattle, and chicken), indicating a zoonotic risk. Therefore, the villagers require proper health education, especially regarding the prevention of parasitic infection, to improve their personal hygiene and community health. Further studies are required to investigate the Blastocystis STs in the animals living in these villages. PMID:27658597

  9. Drinking water is a significant predictor of Blastocystis infection among rural Malaysian primary schoolchildren.

    Science.gov (United States)

    Abdulsalam, Awatif M; Ithoi, Init; Al-Mekhlafi, Hesham M; Ahmed, Abdulhamid; Surin, Johari; Mak, Joon-Wah

    2012-07-01

    Blastocystis infection has a worldwide distribution especially among the disadvantaged population and immunocompromised subjects. This study was carried out to determine the prevalence and the association of Blastocystis infection with the socio-economic characteristics among 300 primary schoolchildren, living in rural communities in Lipis and Raub districts of Pahang state, Malaysia. Stool samples were collected and examined for the presence of Blastocystis using direct smear microscopy after in vitro cultivation in Jones' medium. The overall prevalence of Blastocystis infection was found to be as high as 25.7%. The prevalence was significantly higher among children with gastrointestinal symptoms as compared to asymptomatic children (x2 =4.246; P=0.039). Univariate and multivariate analyses showed that absence of a piped water supply (OR=3.13; 95% CI=1.78, 5.46; PBlastocystis infection. In conclusion, Blastocystis is prevalent among rural children and the important factors that determine the infection were the sources of drinking water and mothers' educational level. Interventions with provision of clean water supply and health education especially to mothers are required. PMID:22444778

  10. Prevalence and Risk Factors for Blastocystis Infection Among Children and Caregivers in a Child Care Center, Bangkok, Thailand.

    Science.gov (United States)

    Pipatsatitpong, Duangnate; Leelayoova, Saovanee; Mungthin, Mathirut; Aunpad, Ratchaneewan; Naaglor, Tawee; Rangsin, Ram

    2015-08-01

    In September 2009, a cross-sectional study was conducted to evaluate parasitic infections in a child care center in Khlong Toei, Bangkok, Thailand. Of 503 children and staff members, 258 (51.3%) stool samples and questionnaires were obtained. The most common parasitic infection was Blastocystis sp. (13.6%). Blastocystis sp. subtype 3 was predominantly found (80.0%), followed by subtypes 2 (12.0%) and 1 (8.0%). The prevalence of Blastocystis infection varied among different age groups. The prevalence of Blastocystis infection in non-HIV-infected children aged < 10 and 10-19 years were 14.5% and 10.3%, respectively, which were not significantly different. All 31 HIV-infected children were not infected with Blastocystis sp. The most likely reason could be the result of properly using prevention measures for this specific group.

  11. The Effect of Hominis Placenta Herbal Acupuncture on Bell's palsy

    Directory of Open Access Journals (Sweden)

    Yun Jeong-hun

    2000-07-01

    Full Text Available This report was done to observe the effect of Hominis placenta herbal acupuncture on Bell's palsy. The study group comprised 16 patients who arrived at Woo-suk university oriental hospital from January, 1999 till January, 2000 for Bell's palsy. All patients were divided into two group. One was herbal acupunture group, and the other was control group. Acupunture group was done herbal acupuncture therapy on the facial acupuncture points. Followings are achievement and a term of each group. In herbal acupuncture group, 100% motor recovery was 7 case, 75% was 1 case, and 25% motor recovery term was 7.38±5.21 days, 50% was 11.00±6.16 days, 75% was 15.13±9.55 days, 100% was 23.14±7.97 days. In control group, 100% motor recovery was 4 case, 75% was 2 case, 25% below was 2 case and 25% motor recovery term was 11.17±4.96days, 50% was 18.17±6.82 days, 75% was 29.50±6.95 days, 100% was 44.00±11.49 days. The above results indicate that Hominis placenta herbal acupuncture is a useful effect on Bell's palsy. thus, continuous herbal acupunture study will be needed for more clinical application on Bell' palsy.

  12. Efficacy of abamectin injection against Dermatobia hominis in cattle.

    Science.gov (United States)

    Cruz, J B; Benitez-Usher, C; Cramer, L G; Gross, S J; Kohn, A B

    1993-01-01

    The efficacy of abamectin 1%, when injected subcutaneously in cattle at a dose of 200 micrograms/kg body weight, against the larval stages (grubs) of the fly Dermatobia hominis was evaluated in two trials in endemic areas of Brazil and Argentina. Eighteen Holstein x Brahman castrated males and 16 Brahman-cross with natural infestations were used. Larvae were counted by instar in situ on both sides of each animal before treatment, and were expressed, identified as to stage and classified as live or dead 10 days after treatment. Further larval counts were made periodically until day 79 to evaluate the degree of reinfestation and the stage of larval development. Reinfestation was first detected in the abamectin-treated cattle on day 44. Live larvae were found on 6-8 (Argentina) and on all (Brazil) controls at each post-treatment examination. The difference in numbers of live larvae between treatment groups was statistically significant (P < 0.05) at all post-treatment examinations. These data show that abamectin at a dose of 200 micrograms/kg body weight is highly effective in the treatment and control of established parasitic stages of D. hominis in cattle. No adverse reactions were observed in any of the treated animals. PMID:8493240

  13. Investigation of infectious status of blastocystis hominis in Guangxi%180例就诊者人芽囊原虫的感染情况调查

    Institute of Scientific and Technical Information of China (English)

    刘莹; 钱程; 陈相任; 曾德权; 何爱娟; 杨远花; 卢作超; 刘登宇

    2008-01-01

    目的 了解人群中人芽囊原虫感染情况,为人芽囊原虫防治工作提供指导.方法 收集医院就诊者粪便,用酸醚沉淀法处理后行碘染色,在高倍镜下查找虫体、观察形态.结果 共检查了180例粪便标本,人芽囊原虫检出率为22.78%,男女受检者检出率分别为18.75%和32.69%,差异有显著性(P<0.05).受检者年龄分布为1~85岁,高发年龄为21~50岁.镜下所见的人芽囊原虫以颗粒型为主.结论 就诊病人中人芽囊原虫的感染率较高,且女性感染率高于男性.

  14. Clinical investigation of infectious status of Blastocystis hominis in Guangxi%人芽囊原虫感染的临床观察

    Institute of Scientific and Technical Information of China (English)

    田春林; 何登贤; 万孝玲

    2006-01-01

    目的 了解广西人芽囊原虫感染的发病特点及临床表现.方法 收集门诊患者的新鲜大便,用生理盐水涂片法检查人芽囊原虫和其他寄生虫,对疑似阳性的标本再用碘液染色法镜检鉴别并计数;询问感染者的病史、生活环境等.结果 检查就诊者粪便标本1 041份,人芽囊原虫感染率为9.89%,年龄分布在13~84岁,男、女感染率分别为10.1%和9.7%,差异无显著性意义(P=0.801);农村与城镇居民感染率分别为10.8%和9.0%,差异亦无显著性意义(P=0.314).103例阳性病例中轻症者占78.64%,主要表现为间歇性腹泻;重症者占21.36%,出现经常性的腹泻、腹痛、厌食、粘液大便等症状,病程较长.结论 人芽囊原虫是广西地区人群重要的消化道寄生虫,可引发腹泻、腹痛等消化道症状.

  15. 婴儿感染人芽囊原虫1例报告%A case of infant infected by Blastocystis hominis

    Institute of Scientific and Technical Information of China (English)

    刘登宇; 卢作超; 云翔

    2006-01-01

    患儿,女性,4月龄,湖南省常德市临澧城关镇人。因无明显诱因血样硬,于2006年5月8日来 广西医科大学一附院儿科就诊。大便3次/d,有腥臭味,较锋稀烂,伴少许黏液,无浓性物,量中等,同时伴呕吐(非喷射状),无咖啡样物,进食即吐,无明显烦燥和哭闹。

  16. 不同环境中人芽囊原虫包囊的结构观察%STRUCTURAL OBSERVATION OF BLASTOCYSTIS HOMINIS CYSTS IN DIFFERENT ENVIRONMENT

    Institute of Scientific and Technical Information of China (English)

    苏水莲; 陈桂凤; 李娟; 刘志春; 廖华

    2003-01-01

    目的观察人芽囊原虫包囊在不同环境中的光学和超微结构的形态特点. 方法从粪便分离人芽囊原虫,转种至改良的Jones培养基培养72 h后,再转种至混合纤维素脂微孔滤膜成囊培养基和Suresh成囊培养基中培养.采用光学和电子显微镜对培养基中与粪便中的包囊进行光学和超微结构的观察. 结果光镜下观察,新鲜粪便中的包囊微小圆球形,具有折光性,不同的个体大小悬殊,HE染色可显示囊壁有3层.用Suresh成囊培养基培养的包囊圆形或椭圆形,中心体色淡,内有大小不等的孢子体.混合纤维素脂微孔滤膜培养的包囊均较大,有空泡型、颗粒型、薄壁与厚壁包囊之分.透射电镜下观察,无纤维层的裸露包囊形态大都是圆形或卵圆形.包囊直径为14 μm.包囊呈典型的人芽囊原虫核,其线粒体较多,胞质中糖原呈大小不一块状. 结论包囊在不同环境中的形态基本相同、大小不一、结构多型.

  17. A Preliminary Study on Preservation Methods of Blastocystis hominis%人芽囊原虫保存方法的初步研究

    Institute of Scientific and Technical Information of China (English)

    田春林; 万孝玲; 刘登宇; 何登贤

    2007-01-01

    目的 观察冻存剂、温度等因素对人芽囊原虫活力的影响,探索理想的人芽囊原虫保存方法.方法 从患者阳性粪便中分离人芽囊原虫,分装到2 ml无菌冻存管内,分别加入10%二甲基亚砜(dimethylsulfoxide,DMS0)、40%丙三醇(GL)和15%乙二醇(EG)作冻存剂,放置于不同的温度下保存,用台盼蓝染色法和原虫培养法测定细胞的活力和增殖能力.结果 虫体在室温(18℃~20℃)下可存活3周,在4℃~6℃存活不到1周.加入冻存剂后,置-20℃和液氮(-196℃)下可存活3个月以上.用40%GL作冻存剂,于液氮低温下冻存的虫体保存半年后复苏,活力仍达41.7%,培养72 h后多见分裂相细胞.结论 应用40%GL作为冻存剂,在液氮中低温保存人芽囊原虫效果较佳.4℃不适于保存人芽囊原虫.

  18. 上海市不同人群人芽囊原虫感染调查%Investigation on the infection of Blastocystis hominis in various populations in Shanghai, China

    Institute of Scientific and Technical Information of China (English)

    张小萍; 马杏宝; 李兰花; 朱倩; 傅英华; 马晓疆; 吕山; 洪国宝; 何艳燕; 王真瑜

    2008-01-01

    目的 了解上海市不同人群人芽囊原虫感染情况.方法 在上海市6个区收集居民新鲜粪便, 采用Ringer溶液37 ℃恒温条件下培养人芽囊原虫,计算人芽囊原虫感染率.结果 检查居民2 984人,人芽囊原虫阳性者88人,感染率2.95%.上海市各区人群感染率间差异无统计学意义(χ2 =9.90,P>0.05);青浦区男性感染率为5.56%,女性感染率1.57%,差异有统计学意义(χ2=5.38,P<0.05),其他5个区男、女性感染率差异无统计学意义(χ2 =0.08~0.54,P均>0.05);农民感染率为6.87%,与其他职业人群比较差异有统计学意义(χ2=18.57,P<0.05).结论 上海市人群对人芽囊原虫普遍易感,以农民感染率最高.体外培养法敏感性较高,可用于人芽囊原虫感染的流行病学调查.

  19. 无症状人芽囊原虫感染调查及PCR鉴定%Survey on Asymptomtic Blastocystis Hominis and Its PCR Identification

    Institute of Scientific and Technical Information of China (English)

    张红卫; 颜秋叶; 贺丽君; 苏云普; 李文; 薛长贵; 王东

    2006-01-01

    目的调查当地居民人芽囊原虫感染情况,用PCR鉴定方法进行初步的分子生物学研究.方法收集当地居民粪便标本,碘染色法镜检人芽囊原虫,体外分离培养并观察其形态及繁殖方式,提取人芽囊原虫基因组DNA,用针对人芽囊原虫SSu rDNA的特异性引物进行PCR鉴定.结果共调查415例当地居民,镜检发现4例阳性,均为无症状人芽囊原虫感染者,阳性率0.96%,未见其他寄生虫合并感染.分离培养观察形态主要为空泡型和颗粒型,未发现阿米巴型和包囊型.繁殖方式以二分裂为主,偶见出芽生殖及三分裂,未发现裂体生殖.PCR扩增获得特异性的阳性条带.结论无症状人芽囊原虫感染率较低,可以通过扩增其SSu rDNA进行鉴定.

  20. Establishment animal model of blastocystis hominis infection on rats%人芽囊原虫感染大鼠模型的建立

    Institute of Scientific and Technical Information of China (English)

    李娟; 邓婷; 李小花; 曹镐禄; 李小波; 严宜明

    2013-01-01

    目的:建立人芽囊原虫感染的大鼠实验动物模型,为致病性研究奠定基础.方法:将32只雄性SPF级SD大鼠随机分成对照组和低剂量、中剂量、高剂量实验组,每组8只.低、中、高剂量实验组按102个/mL、103个/mL、104个/mL经灌胃饲喂包囊液1mL,对照组则饲喂等体积的灭菌生理盐水.饲喂第2天开始检查粪便,隔天一次.2周后解剖全部大鼠,并分别制作盲肠与结肠病理切片,HE染色观察盲肠与结肠组织的病理学变化并评分.结果:高、中、低剂量组粪检均查见芽囊原虫滋养体或包囊,盲肠和结肠组织也出现芽囊原虫侵入、粘膜脱落、炎症细胞浸润等病理学改变.实验组与对照组大鼠的病理评分比较均有显著性差异(P<0.05),但各剂量组之间差异无统计学意义(P>0.05).结论:经口饲喂小剂量包囊,即可成功建立芽囊原虫感染大鼠动物模型.

  1. 赣州市人芽囊原虫感染的流行病学调查分析%Survey epidemiology of Blastocystis hominis infection in Ganzhou

    Institute of Scientific and Technical Information of China (English)

    苏子林; 苏水莲; 吴中发; 廖华; 廖勇; 谢琼珺; 张青生

    2008-01-01

    目的 了解赣州市人芽囊原虫病流行情况.方法 随机抽取江西省赣州市5个县(市)20个调查点,调查不同人群、6种家禽家畜人芽囊原虫感染率,粪检采用生理盐水涂片法.采集生活污水饮用水及自然水源水样,用自然沉淀法查人芽囊原虫污染程度.结果 共检查不同人群1336人,查出人芽囊原虫感染99人,感染率为7.41%.采集5种家禽家畜粪便628份,查出人芽囊原虫67份,平均感染率为10.67%.调查3种水体标本304份,阳性11份,占3.62%.结论 赣州市人群与6种家禽家畜均存在人芽囊原虫感染,3种水体均受到人芽囊原虫污染.

  2. Possible involvement of Mycoplasma hominis in inhibiting the formation of biofilms by uropathogenic Escherichia coli (UPEC).

    Science.gov (United States)

    Oh, Sangnam; Go, Gwang-Woong; Choi, Nag-Jin; Oh, Sejong; Kim, Younghoon

    2013-01-01

    Here we examined the involvement of Mycoplasma hominis in the formation of biofilms by uropathogenic Escherichia coli (UPEC) strain CFT073. Initially, we thought that M. hominis does not affect the fitness of UPEC, including the growth and production of signaling molecules, such as autoinducer-2 and indole. We found, however, that the presence of M. hominis significantly decreased the degree of biofilm formation by UPEC CFT073 (approximately a 60% reduction for 10(5) ccu/mL of M. hominis as compared with UPEC alone). We also found that it had a slight effect in inhibiting the attachment and cytotoxicity of UPEC CFT073. These findings are specific to these UPEC strains rather than to enterohemorrhagic E. coli (EHEC) strains, found in normal intestinal flora. In addition, we performed whole-transcriptome profiling and quantitative real-time polymerase chain reaction (qRT-PCR) analysis. This indicated that the PhoPQ system and the anti-termination protein (encoded by ybcQ) were involved in the reduction of biofilm formation by M. hominis (corroborated by qRT-PCR). Furthermore, our results indicate that M. hominis raises the degree of transcription of toxin genes, including hha and pasT. Hence, we suggest a possible role of M. hominis in affecting the formation of biofilms by UPEC in the urinary tract.

  3. Prevalence of blastocystis in shelter-resident and client-owned companion animals in the US Pacific Northwest.

    Directory of Open Access Journals (Sweden)

    Craig G Ruaux

    Full Text Available Domestic dogs and cats are commonly infected with a variety of protozoan enteric parasites, including Blastocystis spp. In addition, there is growing interest in Blastocystis as a potential enteric pathogen, and the possible role of domestic and in-contact animals as reservoirs for human infection. Domestic animals in shelter environments are commonly recognized to be at higher risk for carriage of enteropathogens. The purpose of this study was to determine the frequency of infection of shelter-resident and client-owned domestic dogs and cats with Blastocystis spp in the Pacific Northwest region of the USA. Fecal samples were collected from 103 shelter-resident dogs, 105 shelter-resident cats, 51 client-owned dogs and 52 client-owned cats. Blastocystis were detected and subtypes assigned using a nested PCR based on small subunit ribosomal DNA sequences. Shelter-resident animals were significantly more likely to test positive for Blastocystis (P<0.05 for dogs, P = 0.009 for cats. Sequence analysis indicated that shelter-resident animals were carrying a variety of Blastocystis subtypes. No relationship was seen between Blastocystis carriage and the presence of gastrointestinal disease signs in either dogs or cats. These data suggest that, as previously reported for other enteric pathogens, shelter-resident companion animals are a higher risk for carriage of Blastocystis spp. The lack of relationship between Blastocystis carriage and intestinal disease in shelter-resident animals suggests that this organism is unlikely to be a major enteric pathogen in these species.

  4. [Effects of the symbiosis of Trichomonas vaginalis with Mycoplasma hominis on ferredoxin gene].

    Science.gov (United States)

    Liu, Xiaodong; Wen, Wenjing; Xue, Changgui

    2011-08-01

    We isolated 30 Trichomonas vaginalis for the PCR detection from the gynecological outpatients in the Affiliated Hospital of Zhengzhou University using the specific 16s rDNA primers of Mycoplasma hominis. The results showed that there were 25 cases of Mycoplasma hominis infection, with the infection rate of 83.33%. This gave a clew that the symbiosis of Trichomonas vaginalis with Mycoplasma hominis may be of certain generality in China. We sequenced the ferredoxin gene of 10 Trichomonas vaginalis where 5 Mycoplasma hominis were positive and five negative, and found that the ferredoxin (Fd) gene of the 10 Trichomonas vaginalis were exactly the same. But compared to the genes in the GenBank, a comparative analysis of the gene revealed that there were 3 more ctg bases at the 200th position of encoding leucine, but this did not lead to changes in reading frame. The gene homology was 99%.

  5. Mycoplasma hominis septic arthritis and common variable immunodeficiency in a postpartum patient:a case report

    Institute of Scientific and Technical Information of China (English)

    Randy A. McCool

    2012-01-01

    Postpartum patients with an unrecognized primary immunodeficiency disease, including common variable immunodeficiency, demonstrate increased susceptibility to Mycoplasma hominis infection. Diagnosis, treatment, and clinical course in a postpartum patient presenting with joint pain and episodic fever are presented.

  6. The presence of Mycoplasma hominis in isolates of Trichomonas vaginalis impacts significantly on DNA fingerprinting results.

    Science.gov (United States)

    Xiao, J C; Xie, L F; Zhao, L; Fang, S L; Lun, Z R

    2008-03-01

    The genetic characterization of Trichomonas vaginalis (Protista: Trichomonadidae), the causative agent of trichomoniasis in humans, is central to understanding the epidemiology, treatment, drug resistance, and virulence as well as the diagnosis and control of this parasite. Various molecular approaches, including DNA fingerprinting, have been employed for this purpose, and random amplification of polymorphic DNA (RAPD) continues to be utilized. However, little attention has been paid to the fact that some T. vaginalis populations can harbor symbiotic Mycoplasma hominis and/or other agents, which could cause artifacts in the RAPD results. In the present study, we demonstrate clearly that the presence of M. hominis from T. vaginalis isolates impacts significantly on RAPD results and on the subsequent analyses and interpretation of data sets. Moreover, symbiotic M. hominis displays an isolate-to-isolate variability in RAPD profile before elimination, suggesting a variability of M. hominis infection. PMID:18058131

  7. Development of a new PCR protocol to detect and subtype Blastocystis spp. from humans and animals

    Science.gov (United States)

    Blastocystis spp. is commonly found in the feces of humans worldwide. Infection has been reported as asymptomatic, acute symptomatic, and chronic symptomatic. This wide range of responses to infection could be related to the genetic diversity of morphologically indistinguishable specimens obtained...

  8. Seeing the Whole Elephant: Imaging Flow Cytometry Reveals Extensive Morphological Diversity within Blastocystis Isolates.

    Science.gov (United States)

    Yason, John Anthony; Tan, Kevin Shyong Wei

    2015-01-01

    Blastocystis is a common protist isolated in humans and many animals. The parasite is a species complex composed of 19 subtypes, 9 of which have been found in humans. There are biological and molecular differences between Blastocystis subtypes although microscopy alone is unable to distinguish between these subtypes. Blastocystis isolates also display various morphological forms. Several of these forms, however, have not been properly evaluated on whether or not these play significant functions in the organism's biology. In this study, we used imaging flow cytometry to analyze morphological features of Blastocystis isolates representing 3 subtypes (ST1, ST4 and ST7). We also employed fluorescence dyes to discover new cellular features. The profiles from each of the subtypes exhibit considerable differences with the others in terms of shape, size and granularity. We confirmed that the classical vacuolar form comprises the majority in all three subtypes. We have also evaluated other morphotypes on whether these represent distinct life stages in the parasite. Irregularly-shaped cells were identified but all of them were found to be dying cells in one isolate. Granular forms were present as a continuum in both viable and non-viable populations, with non-viable forms displaying higher granularity. By analyzing the images, rare morphotypes such as multinucleated cells could be easily observed and quantified. These cells had low granularity and lower DNA content. Small structures containing nucleic acid were also identified. We discuss the possible biological implications of these unusual forms. PMID:26618361

  9. Prevalence and subtype distribution of Blastocystis in healthy individuals in Sharjah, United Arab Emirates.

    Science.gov (United States)

    AbuOdeh, Raed; Ezzedine, Sinda; Samie, Amidou; Stensvold, Christen Rune; ElBakri, Ali

    2016-01-01

    Blastocystis is estimated to be one of the most common parasites of the intestinal tract of humans, comprising multiple subtypes (ST). Meanwhile, the distribution of Blastocystis ST in many communities and countries remains unknown. In the present work, we aimed to identify the prevalence of Blastocystis and the ST distribution in human stool samples collected from healthy expatriates from different geographical regions and residing in Sharjah, United Arabian Emirates (UAE). A total of 133 samples were screened and subtyped using partial small subunit ribosomal RNA gene sequencing. Fifty-nine (44.4%) samples were identified as positive. Among these, 39 were successfully sequenced and subtyped. The ST distribution was as follows: ST3, 58.9% (23/39); ST1, 28.2% (11/39); and ST2, 7.6% (3/39). No correlation between geographic origin and infection (χ(2)=11.006; P=0.528) nor gender and infection (χ(2)=1.264; P=0.261) was observed. The data were compared with those available for other Middle Eastern and North African neighboring countries. This study is the first to provide data concerning the prevalence of Blastocystis and the frequency of various STs in the UAE, confirming the absence of ST4 and the commonness of ST1, ST2, and ST3 in this geographical region. PMID:26611823

  10. Organelles in Blastocystis that blur the distinction between mitochondria and hydrogenosomes.

    Science.gov (United States)

    Stechmann, Alexandra; Hamblin, Karleigh; Pérez-Brocal, Vicente; Gaston, Daniel; Richmond, Gregory S; van der Giezen, Mark; Clark, C Graham; Roger, Andrew J

    2008-04-22

    Blastocystis is a unicellular stramenopile of controversial pathogenicity in humans. Although it is a strict anaerobe, Blastocystis has mitochondrion-like organelles with cristae, a transmembrane potential and DNA. An apparent lack of several typical mitochondrial pathways has led some to suggest that these organelles might be hydrogenosomes, anaerobic organelles related to mitochondria. We generated 12,767 expressed sequence tags (ESTs) from Blastocystis and identified 115 clusters that encode putative mitochondrial and hydrogenosomal proteins. Among these is the canonical hydrogenosomal protein iron-only [FeFe] hydrogenase that we show localizes to the organelles. The organelles also have mitochondrial characteristics, including pathways for amino acid metabolism, iron-sulfur cluster biogenesis, and an incomplete tricarboxylic acid cycle as well as a mitochondrial genome. Although complexes I and II of the electron transport chain (ETC) are present, we found no evidence for complexes III and IV or F1Fo ATPases. The Blastocystis organelles have metabolic properties of aerobic and anaerobic mitochondria and of hydrogenosomes. They are convergently similar to organelles recently described in the unrelated ciliate Nyctotherus ovalis. These findings blur the boundaries between mitochondria, hydrogenosomes, and mitosomes, as currently defined, underscoring the disparate selective forces that shape these organelles in eukaryotes. PMID:18403202

  11. Occurrence of blastocystis in water of two rivers from recreational areas in malaysia.

    Science.gov (United States)

    Ithoi, Init; Jali, Azman; Mak, J W; Wan Sulaiman, Wan Yusoff; Mahmud, Rohela

    2011-01-01

    This study reports the occurrence of Blastocystis in water from two rivers, Sungai Congkak and Sungai Batu, located in recreational areas in Malaysia. This protozoan was detected in samples from both rivers with an average of 33.3% and 22.1%, respectively. It was detected highest at the downstream (73.8% and 33.8%) followed by midstream (17.5% and 25.0%) and upstream (8.8% and 7.5%) stations, with additionally higher detection during holidays (with average 47.5% and 30.8%) than week days (with average 19.2% and 13.3%), in both rivers, respectively. There was a strong association with the daily activities of locals and visitors, who came for water recreational activities, mainly located between midstream and downstream and was observed to be higher at Sungai Congkak. The detection of Blastocystis in these rivers' water implies that this protozoan could potentially be transmitted to humans by the waterborne route. Pearson correlation analysis showed that their occurrence was significantly correlated with faecal coliforms count; inconsistent correlation with dissolved oxygen, temperature and turbidity and no correlation with pH, conductivity and rainfall for both rivers. The correlation of coliforms and Blastocystis suggests the source of the Blastocystis in the water body is likely to be faecal. PMID:21772980

  12. Prevalence and subtype distribution of Blastocystis in healthy individuals in Sharjah, United Arab Emirates.

    Science.gov (United States)

    AbuOdeh, Raed; Ezzedine, Sinda; Samie, Amidou; Stensvold, Christen Rune; ElBakri, Ali

    2016-01-01

    Blastocystis is estimated to be one of the most common parasites of the intestinal tract of humans, comprising multiple subtypes (ST). Meanwhile, the distribution of Blastocystis ST in many communities and countries remains unknown. In the present work, we aimed to identify the prevalence of Blastocystis and the ST distribution in human stool samples collected from healthy expatriates from different geographical regions and residing in Sharjah, United Arabian Emirates (UAE). A total of 133 samples were screened and subtyped using partial small subunit ribosomal RNA gene sequencing. Fifty-nine (44.4%) samples were identified as positive. Among these, 39 were successfully sequenced and subtyped. The ST distribution was as follows: ST3, 58.9% (23/39); ST1, 28.2% (11/39); and ST2, 7.6% (3/39). No correlation between geographic origin and infection (χ(2)=11.006; P=0.528) nor gender and infection (χ(2)=1.264; P=0.261) was observed. The data were compared with those available for other Middle Eastern and North African neighboring countries. This study is the first to provide data concerning the prevalence of Blastocystis and the frequency of various STs in the UAE, confirming the absence of ST4 and the commonness of ST1, ST2, and ST3 in this geographical region.

  13. Biochemical characterization of a mitochondrial-like organelle from Blastocystis sp. subtype 7.

    Science.gov (United States)

    Lantsman, Yelena; Tan, Kevin S W; Morada, Mary; Yarlett, Nigel

    2008-09-01

    A mitochondrion-like organelle (MLO) was isolated from isotonic homogenates of Blastocystis. The organelle sedimented at 5000 g for 10 min, and had an isopycnic density in sucrose of 1.2 g ml(-1). Biochemical characterization enabled the demonstration of several key enzymes that allowed the construction of a metabolic pathway consisting of an incomplete Krebs cycle linked to the oxygen-sensitive enzymes pyruvate : NADP(+) oxidoreductase (PNO), acetate : succinate CoA transferase (ASCT) and succinate thiokinase (STK), which cumulatively are responsible for recycling CoA and generating ATP. The organelle differs from typical aerobic mitochondria in possessing an oxygen-sensitive PNO that can use FAD(+) or FMN(+) as electron acceptor but is inactive with NAD(+), Spinacia oleracea ferredoxin or Clostridium pasteurianum ferredoxin. A gene with 77 % sequence similarity to the PNO mitochondrion precursor cluster from Euglena gracilis sp[Q941N5] was identified in the Blastocystis genome database. A second cluster with 56 % sequence similarity to the pyruvate : ferredoxin oxidoreductase (PFOR) from Trichomonas vaginalis was also identified, which is in agreement with the concept that the PNO gene arose through the fusion of a eubacterial gene for PFOR with the gene for NADPH : cytochrome p450 reductase. Hydrogenase activity was not detected under the conditions used in this study. The Blastocystis oranelle therefore demonstrates significant biochemical differences from traditional mitochondria and hydrogenosomes, but possesses features of both. Based upon the results of this study, the Blastocystis organelle falls into the category of a MLO. PMID:18757809

  14. Mycoplasma hominis in Cuban Trichomonas vaginalis isolates: association with parasite genetic polymorphism.

    Science.gov (United States)

    Fraga, Jorge; Rodríguez, Nadia; Fernández, Carmen; Mondeja, Brian; Sariego, Idalia; Fernández-Calienes, Aymé; Rojas, Lazara

    2012-07-01

    Trichomonas vaginalis can be naturally infected with intracellular Mycoplasma hominis. This bacterial infection may have implications for trichomonal virulence and disease pathogenesis. The objective of the study was to report the presence of M. hominis in Cuban T. vaginalis isolates and to describe the association between the phenotype M. hominis infected with RAPD genetic polymorphism of T. vaginalis. The Random Amplified Polymorphic DNA (RAPD) technique was used to determine genetic differences among 40 isolates of T. vaginalis using a panel of 30 random primers and these genetic data were correlated with the infection of isolates with M. hominis. The trees drawn based on RAPD data showed no relations with metronidazole susceptibility and significantly association with the presence of M. hominis (P=0.043), which demonstrates the existence of concordance between the genetic relatedness and the presence of M. hominis in T. vaginalis isolates. This result could point to a predisposition of T. vaginalis for the bacterial enters and/or survival. PMID:22584035

  15. Immunopathological assessments of human Blastocystis spp. in experimentally infected immunocompetent and immunosuppresed mice.

    Science.gov (United States)

    Abdel-Hafeez, Ekhlas H; Ahmad, Azza K; Abdelgelil, Noha H; Abdellatif, Manal Z M; Kamal, Amany M; Hassanin, Kamel M A; Abdel-Razik, Abdel-Razik H; Abdel-Raheem, Ehab M

    2016-05-01

    Blastocystis spp., one of the most common parasites colonizing the human intestine, is an extracellular, luminal protozoan with controversial pathogenesis. The host's immune response against Blastocystis spp. infection has also not been defined yet. Therefore, this research aimed to assess the potential pathogenicity of this parasite and its ability to modulate the immune response in experimental infected immunocompetent and immunosuppresed mice. These results demonstrated that the infected immunosuppressed mice were more affected than infected immunocompetent mice. Histopathological examination of the small intestine in the infected immunosuppressed mice showed that Blastocystis spp. infiltrated all the layers. Moreover, the epithelia showed exfoliation and inflammatory cell infiltration in submucosa compared to that of the infected immunocompetent mice. As well, examination of the large intestine of the infected immunosuppressed group showed severe goblet cell hyperplasia. Blastocystis spp. infiltrated all the large intestine layers compared to that of the infected immunocompetent group. Furthermore, there was a significant upregulation of the expression of proinflammatory cytokines: interleukin 12 (IL-12) and tumor necrosis factor alpha (TNF-α) in the infected immunosuppressed mice compared to that of the infected immunocompetent ones (p ≤ 0.004 and p ≤ 0.002, respectively). However, the expression of anti-inflammatory cytokines (IL-4 and IL-10) was significantly downregulated in the infected immunosuppressed group compared to that of the infected immunocompetent group one at 10 days postinfection (p ≤ 0.002 and p ≤ 0.001, respectively). The results of this study revealed that Blastocystis spp. affected the production of pro- and anti-inflammatory cytokines in both groups of mice compared to healthy normal (naive) group. Additionally, these data showed that there was a significant upregulation (p ≤ 0.005) of the locally

  16. Flutuação sazonal de Dermatobia hominis em peles bovinas oriundas de matadouro

    Directory of Open Access Journals (Sweden)

    Brito Luciana Gatto

    2000-01-01

    Full Text Available Realizou-se o estudo do efeito da temperatura, precipitação pluviométrica e da umidade relativa sobre a flutuação sazonal de larvas de Dermatobia hominis em 5.142 peles de bovinos. As peles foram obtidas junto a um matadouro localizado no município de Piraí, estado do Rio de Janeiro, Brasil, durante o período de novembro de 1995 a outubro de 1997. Os maiores picos de peles parasitadas por berne foram observados em novembro de 1995 e dezembro de 1996. Picos menores de infestação foram observados nos meses de março e maio de 1996 e março de 1997, quando também foram observadas altas porcentagens de peles infestadas. O aumento na porcentagem de peles parasitadas acompanhou o aumento da temperatura média mensal, sendo que temperaturas iguais ou maiores que 25ºC se mostraram benéficas ao aumento na porcentagem de peles parasitadas, assim como a precipitação pluviométrica, onde observou-se que períodos de chuvas mensais acima de 200 mm³ favoreceram ao aumento da porcentagem de peles parasitadas. As menores porcentagens de peles parasitadas por berne foram observadas durante o período de junho a outubro de 1996 e de 1997, quando verificou-se temperaturas médias mensais variando entre 21ºC e 22,5ºC, sendo que estas não se mostraram favoráveis ao aumento na porcentagem de peles parasitadas, assim como, precipitações pluviométricas mensais menores que 50 mm³. Das 5.142 peles examinadas, 2.710 (52,7% encontravam-se parasitadas pelo berne.

  17. The Clinical Study on a Case of Transverse Myelitis With Bee Venom and Hominis Placenta Herbal Acupuncture

    Directory of Open Access Journals (Sweden)

    Park Min-ho

    2005-06-01

    Full Text Available Objective : This study was performed to evaluate the treatment of acupuncture therapy including Bee Venom and Hominis Placenta herbal acupuncture on the patient with Transverse myelitis. Methods : We treated the patient with Transverse myelitis by Bee Venom herbal acupuncture at beginning, since then we treated him adding to Hominis Placenta herbal acupuncture. Conclusions : The patient was effectively reduced symptoms with Bee Venom herbal acupuncture, since then he get more effective improvement of symptoms by adding Hominis Placenta herbal acupuncture. Therefore we are able to expect Bee venom and Hominis Placenta herbal acupuncture will be more effective than simply acupuncture on the patient with Transverse myelitis.

  18. Epidémiologie moléculaire, facteurs de risque de transmission et pathogénicité du protiste parasite Blastocystis sp.

    OpenAIRE

    El Safadi, Dima

    2014-01-01

    Blastocystis sp. is an anaerobic parasitic protozoa found in the digestive tract of humans and numerous animals. To date, it is the most common intestinal parasite found in human feces with worldwide distribution. Seventeen subtypes (ST1-ST17) have been described based on the comparison of SSU rRNA gene sequences. Blastocystis infection is associated with various gastrointestinal disorders and many studies suggest a correlation between Blastocystis STs and pathogenicity. My work was developed...

  19. Comparison of the genetic variability of Blastocystis subtypes between human carriers from two contrasting climatic regions of México.

    Science.gov (United States)

    Villegas-Gómez, Isaac; Martínez-Hernández, Fernando; Urrea-Quezada, Alejandro; González-Díaz, Mariana; Durazo, María; Hernández, Jesús; Orozco-Mosqueda, Guadalupe Erendira; Villalobos, Guiehdani; Maravilla, Pablo; Valenzuela, Olivia

    2016-10-01

    Blastocystis sp. is an anaerobic intestinal microorganism commonly identified in the feces of several animals, including humans. Blastocystis exhibits high genetic polymorphism and at least 17 subtypes (ST) have been identified; ST1-ST3 are frequently found in the Americas. Furthermore, in vitro assays have shown that temperature and humidity can affect the viability of Blastocystis cysts. In this study, we describe the genetic variability and genetic differentiation among and within Blastocystis STs in adults and children from the cities of Hermosillo and Morelia cities, which represent arid and humid subtropical climatic regions of México, respectively. Phylogenetic and genetic diversity was assessed by analyzing a region of the small subunit ribosomal DNA (SSU rDNA) gene as a marker. Blastocystis ST3 and ST1 were associated with children from Hermosillo and Morelia, respectively. An analysis of the nucleotide diversity (π) and haplotype polymorphism (θ) indexes showed that they were similar within each ST, but different between ST1 and ST3. Interestingly, the group of symptomatic carriers from Hermosillo showed scarce mean nucleotide diversity compared to the asymptomatic carriers (0.0039±0.0030 and 0.0329±0.0286, respectively). Furthermore, the gene flow and genetic differentiation indexes between the children and adults suggested that the Blastocystis haplotypes in the adult carriers were "highly mobile" among humans, while the haplotypes found in the children were more isolated and genetically differentiated between them. PMID:27476606

  20. Molecular mechanism of fluoroquinolones resistance in Mycoplasma hominis clinical isolates

    Directory of Open Access Journals (Sweden)

    Meng Dong-Ya

    2014-01-01

    Full Text Available To evaluate the molecular mechanism of fluoroquinolones resistance in Mycoplasma hominis (MH clinical strains isolated from urogenital specimens. 15 MH clinical isolates with different phenotypes of resistance to fluoroquinolones antibiotics were screened for mutations in the quinolone resistance-determining regions (QRDRs of DNA gyrase (gyrA and gyrB and topoisomerase IV (parC and parE in comparison with the reference strain PG21, which is susceptible to fluoroquinolones antibiotics. 15 MH isolates with three kinds of quinolone resistance phenotypes were obtained. Thirteen out of these quinolone-resistant isolates were found to carry nucleotide substitutions in either gyrA or parC. There were no alterations in gyrB and no mutations were found in the isolates with a phenotype of resistance to Ofloxacin (OFX, intermediate resistant to Levofloxacin (LVX and Sparfloxacin (SFX, and those susceptible to all three tested antibiotics. The molecular mechanism of fluoroquinolone resistance in clinical isolates of MH was reported in this study. The single amino acid mutation in ParC of MH may relate to the resistance to OFX and LVX and the high-level resistance to fluoroquinolones for MH is likely associated with mutations in both DNA gyrase and the ParC subunit of topoisomerase IV.

  1. Laboratory characteristics of blastocystis invasion in patients with chronic viral hepatitis

    Directory of Open Access Journals (Sweden)

    A. S. Sigidaev

    2011-01-01

    Full Text Available Based on the survey results in 1322 patients found that patients with hronic viral hepatitis (CVH blastocysts are found more often than in the group of individuals without concomitant pathology of the hepatic-biliary system (28.8% vs. 5.5%, respectively. In healthy individuals the blastocyst revealed more often in the summer, peaking in July and August, while in patients with CVH Blastocystis most frequently observed in winter. The high incidence of Blastocystis sp. CVH patients suggests that the pathology of the hepatic-biliary system fosters a supportive environment in the gastrointestinal tract for the colonization of its blastocyst. Molecular biological methods of investigation revealed that the subtype antroponozny blastocysts met in this population, 5.3 times less likely than those without concomitant liver disease.

  2. Colonization with the enteric protozoa Blastocystis is associated with increased diversity of human gut bacterial microbiota

    OpenAIRE

    Audebert, Christophe; Even, Gaël; Cian, Amandine; ,; Safadi, Dima El; Certad, Gabriela; Delhaes, Laurence; Pereira, Bruno; Nourrisson, Céline; Poirier, Philippe; Wawrzyniak, Ivan; Delbac, Frédéric; Morelle, Christelle; Bastien, Patrick; Lachaud, Laurence

    2016-01-01

    Alterations in the composition of commensal bacterial populations, a phenomenon known as dysbiosis, are linked to multiple gastrointestinal disorders, such as inflammatory bowel disease and irritable bowel syndrome, or to infections by diverse enteric pathogens. Blastocystis is one of the most common single-celled eukaryotes detected in human faecal samples. However, the clinical significance of this widespread colonization remains unclear, and its pathogenic potential is controversial. To ad...

  3. Epidemiologic Study of Blastocystis Infection in an Urban Community in the Philippines

    OpenAIRE

    Belleza, Maria Luz B.; Cadacio, Jessa Louise C.; Maridel P. Borja; Solon, Juan Antonio A.; Padilla, Mildred A.; Tongol-Rivera, Pilarita N.; Rivera, Windell L.

    2015-01-01

    Blastocystis has been considered as the most common intestinal parasite in humans and has an augmented impact on public health. However, the prevalence of this parasite in the Philippines has not been determined. To contribute to a better understanding of the epidemiology of this infection, a cross-sectional study aimed at providing the first documented data on the prevalence and correlates, sociodemographic factors, hygiene practices, source of water supply, and dog ownership, associated wit...

  4. Comparison of Sequencing (Barcode Region) and Sequence-Tagged-Site PCR for Blastocystis Subtyping

    Science.gov (United States)

    2013-01-01

    Blastocystis is the most common nonfungal microeukaryote of the human intestinal tract and comprises numerous subtypes (STs), nine of which have been found in humans (ST1 to ST9). While efforts continue to explore the relationship between human health status and subtypes, no consensus regarding subtyping methodology exists. It has been speculated that differences detected in subtype distribution in various cohorts may to some extent reflect different approaches. Blastocystis subtypes have been determined primarily in one of two ways: (i) sequencing of small subunit rRNA gene (SSU-rDNA) PCR products and (ii) PCR with subtype-specific sequence-tagged-site (STS) diagnostic primers. Here, STS primers were evaluated against a panel of samples (n = 58) already subtyped by SSU-rDNA sequencing (barcode region), including subtypes for which STS primers are not available, and a small panel of DNAs from four other eukaryotes often present in feces (n = 18). Although the STS primers appeared to be highly specific, their sensitivity was only moderate, and the results indicated that some infections may go undetected when this method is used. False-negative STS results were not linked exclusively to certain subtypes or alleles, and evidence of substantial genetic variation in STS loci was obtained. Since the majority of DNAs included here were extracted from feces, it is possible that STS primers may generally work better with DNAs extracted from Blastocystis cultures. In conclusion, due to its higher applicability and sensitivity, and since sequence information is useful for other forms of research, SSU-rDNA barcoding is recommended as the method of choice for Blastocystis subtyping. PMID:23115257

  5. Mycoplasma hominis: an incidental but significant finding by routine bacteriological culture

    DEFF Research Database (Denmark)

    Gertsen, Jan Berg; Schønheyder, Henrik Carl

    2009-01-01

    bacteriological culture during a 4-year period. Methods: Dpt. of Clinical Microbiology, Aalborg Hospital serves a population of 0.5 mio. Aerobic bacteriologic cultures are routinely carried out on 5% horse blood agar and chocolate agar (SSI Diagnostika, DK) at 35 °C in 5% CO2. The finding of translucent, pinpoint...... Serum Institut, Copenhagen (by courtesy to Jørgen Skov). Results: The four patients were immunocompetent women (23-56 years of age) without significant comorbidity (Table). In all patients M. hominis were obtained in pure culture. At the time of diagnosis three patients had abscesses...... by resolution of symptoms and normalisation of CRP (median 9 days). Conclusion: M. hominis is a rare finding by prolonged incubation of conventional blood agar. A pathogenic role of M. hominis was supported by the lack of clinical response to surgical drainage and prolonged empirical antibiotic therapy...

  6. Hematoma and abscess formation caused by Mycoplasma hominis following cesarean section

    Directory of Open Access Journals (Sweden)

    Hisato Koshiba, Akemi Koshiba

    2011-01-01

    Full Text Available Hisato Koshiba1,2, Akemi Koshiba1,2, Yasushi Daimon3, Toshifumi Noguchi1,2, Kazuhiro Iwasaku2, Jo Kitawaki21Department of Obstetrics and Gynecology, Kyoto Prefectural Yosanoumi Hospital, Kyoto, Japan; 2Department of Obstetrics and Gynecology, Kyoto Prefectural University of Medicine, Graduate School of Medical Science, Kyoto, Japan; 3Department of Clinical Laboratory, Kyoto Prefectural Yosanoumi Hospital, Kyoto, JapanAbstract: Mycoplasma species cannot be identified by routine bacteriological culture methods and are resistant to common antimicrobial agents. Mycoplasma hominis usually colonizes the lower urogenital tract and causes pyelonephritis, pelvic inflammatory disease, chorioamnionitis, rupture of fetal membranes, preterm labor, postpartum fever, postabortal fever, and neonatal infection. This organism is highly prevalent in cervicovaginal cultures of sexually active women. M. hominis, M. genitalis, Ureaplasma urealyticum, and U. parvum may invade and infect placental and fetal tissues, leading to adverse pregnancy outcomes. M. hominis occasionally causes nongenitourinary infection of the blood, wounds, central nervous system, joints, or respiratory tract. We present a case of a 27-year-old woman who developed abdominal wound hematoma and abscess after cesarean section. The wound was drained, but her high fever persisted, in spite of antibiotic treatment using flomoxef sodium and imipenem•cilastatin sodium. Because the exudate exhibited M. hominis growth in an anaerobic environment, we administered the quinolone ciprofloxacin. This therapy resolved her fever, and her white blood cell count and C-reactive protein level diminished to the normal ranges. To our knowledge, there are four published articles regarding the isolation of M. hominis from postcesarean incisions. Based on the current study and the literature, infection by this pathogen may cause hematoma formation with or without abscess after cesarean section or in immunosuppressed

  7. Cloning, sequencing and variability analysis of the gap gene from Mycoplasma hominis

    DEFF Research Database (Denmark)

    Mygind, Tina; Jacobsen, Iben Søgaard; Melkova, Renata;

    2000-01-01

    The gap gene encodes the glycolytic enzyme glyceraldehyde 3-phosphate dehydrogenase (GAPDH). The gene was cloned and sequenced from the Mycoplasma hominis type strain PG21(T). The intraspecies variability was investigated by inspection of restriction fragment length polymorphism (RFLP) patterns...... after polymerase chain reaction (PCR) amplification of the gap gene from 15 strains and furthermore by sequencing of part of the gene in eight strains. The M. hominis gap gene was found to vary more than the Escherichia coli counterpart, but the variation at nucleotide level gave rise to only a few...

  8. Strain-Dependent Induction of Human Enterocyte Apoptosis by Blastocystis Disrupts Epithelial Barrier and ZO-1 Organization in a Caspase 3- and 9-Dependent Manner

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    Zhaona Wu

    2014-01-01

    Full Text Available Blastocystis is an emerging protistan parasite colonizing the human intestine. It is frequently reported to cause general intestinal symptoms of vomiting, diarrhea, and abdominal pain. We recently demonstrated that Blastocystis rearranged cytoskeletal proteins and induced intestinal epithelial barrier compromise. The effect of Blastocystis on enterocyte apoptosis is unknown, and a possible link between microbially induced enterocyte apoptosis and increased epithelial permeability has yet to be determined. The aim of this study is to assess if Blastocystis induces human enterocyte apoptosis and whether this effect influences human intestinal epithelial barrier function. Monolayers of polarized human colonic epithelial cell-line Caco-2 were incubated with Blastocystis subtype 7 and subtype 4. Assays for both early and late markers of apoptosis, phosphatidylserine externalization, and nuclear fragmentation, respectively, showed that Blastocystis ST-7, but not ST-4, significantly increased apoptosis in enterocytes, suggesting that Blastocystis exhibits host specificity and strain-to-strain variation in pathogenicity. ST-7 also activated Caco-2 caspases 3 and 9 but not 8. ST-7 induced changes in epithelial resistance, permeability, and tight junction (ZO-1 localization. Pretreatment of Caco-2 monolayers with a pan-caspase inhibitor z-VAD-fmk significantly inhibited these changes. This suggests a role for enterocyte apoptosis in Blastocystis-mediated epithelial barrier compromise in the human intestine.

  9. Charcterization of Type Ⅱ Topoisomerase Gene Mutations in Clinical Isolates of Mycoplasma Hominis Resistant to Fluoroquinolones

    Institute of Scientific and Technical Information of China (English)

    吴移谋; 张文波; 姚艳冰

    2002-01-01

    Objective: To analyze type Ⅱ topoisomerase genes inclinical isolates of fluoroquinolone-resistant Mycoplasmahominis. Methods: Eight isolates of M.hominis cross resistant to 6fluoroquinolones were selected from 103 clinical strains ofM.hominis using a broth microdilution method. Type IItopoisomerase genes were amplified by PCR and directlysequenced. Nucleotide sequences were compared to sequencesfrom a susceptible strain (M.hominis PG2I). Results: MICs of resistant Mh isolates were 4- to 512-fold higher than MICs from the susceptible reference strain.Sequence comparison revealed a C to T change at 113nt ingyrA QRDR led to the substitution of Ser83 by Leucine and noamino acid change in gyrB. A change of G to T at 134nt inparC QRDR led to the substitution of Ser80 by Isoleucine anda G to A change at 70nt in parE QRDR led to the substitutionof Aspartic acid by Asparagine. Conclusion: These results suggest that a C to T change atll3nt in gyrA, a G to T change at 134nt in parC and a G to Achange at 70nt in patrE are associated with fluoroquinoloneresistance of M.hominis.

  10. The Effects of the Hominis Placenta Herbal acupuncture on Sleep pattern disturbance

    Directory of Open Access Journals (Sweden)

    Youn Hyoun-min

    2005-02-01

    Full Text Available Objective : This study has been designed and performed to identify the effects of Hominis Placenta herbal acupuncture which is usually used in reducing sleep pattern disturbances. Methods : The study subjects studied included 48 patients who were admitted in hospital located in Pusan, and they were classified into 2 groups : 25 patients in the experimental group who injected Hominis Placenta herbal acupuncture and 23 patients in the control group who were treated by acupuncture. The both group injected on GB20, GB12 and HT7 for 5 days without medicine. The sleep pattern disturbance score was measured by using 15 questions according to Korean Sleep Scale A(Oh, Jin Joo. Song, Mi Soon. Kim, Shin Mi. 1998. Results & conclusions : The sleep pattern disturbance score of the experimental group who injected Hominis Placenta herbal acupuncture was significantly lower than that of the control group. (t= 7.00 p= .00 These results provided that Hominis Placenta herbal acupuncture of GB20, GB12 and HT7 was effective for relieving sleep pattern disturbances, it is need more sample's number and more treatmentt's duration.

  11. Colonization of Mycoplasma hominis and Ureaplasma urealyticum in pregnant women and their transmission to offspring.

    Directory of Open Access Journals (Sweden)

    Behnam Sobouti

    2014-08-01

    Full Text Available Mycoplasma hominis and Ureaplasma urealyticum are important opportunistic pathogens that cause urogenital infections and accelerated newborn delivery in pregnant women. Moreover genital mycoplasmas have been implicated in different neonatal diseases such as pneumonia, sepsis and meningitis. This study was conducted to find out the prevalence and transmission rate of these two organisms in pregnant women and their neonates.Nasotracheal and pharyngeal specimens of 165 newborns hospitalized at Neonatal Intensive Care Unit (NICU of Rasoul Akram Hospital (during 2010 - 2011 were assessed by PCR to detect M. hominis and U. urealyticum. Moreover, PCR of vaginal specimens from their mothers were obtained to determine the prevalence of these organisms in pregnant women and rate of transmission to their newborns. Data were analyzed using SPSS software.Totally, the results of PCR were positive in 33 newborns (20%. Vaginal colonization among the mothers was found to be 15% (25/165 for U. urealyticum and 15% (25/165 for M. hominis. The transmission rate to their infants was 72% and 60% for U. urealyticum and M. hominis, respectively.These data indicate that vertical transmission of mycoplasma and ureaplasma are prevalent in newborns. Since these organisms cause serious infections in neonates, it would be better to perform screening tests in pregnant women before the delivery in order to prevent transmission to neonates and consequent infections and morbidities among them.

  12. Biological Activities of Hominis Placenta Herbal Acupuncture prepared by Hydrochloric Acid Hydrolysis

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    Geun-young Seo

    2010-06-01

    Full Text Available Reactive Oxygen Species(ROS are continuously produced at a high rate as a by-product of aerobic metabolism. Since tissue damage by free radical increases with age, the reactive oxygen species(ROS such as hydrogen peroxide(H2O2, nitric oxide(NO. Several lines of evidence provided that ROS appears to cause to develop aging-related various diseases such as cancer, arthritis, cardiovascular disease. In this study, we have conducted to investigate the biological activities of Hominis Placenta Herbal Acupuncture by measuring total polyphenol content, DPPH radical scavenging, ABTS radical scavenging, Superoxide dismutase(SOD-like activity, Nitrite scavenging ability in vitro. The total polyphenol contents of Hominis Placenta Herbal Acupuncture was 24.6㎖/㎖. Elctron donation ability on DPPH was 49.4%. The 2,2'-azinobis-3-ehtlbezothiazoline-6-sulfonic acid radical decolorization (ABTS was 50.01%, similar to the DPPH free radical scavenging. The superoxide dismutase (SOD-like activities of hominis placenta herbal acupuncture was 50.876%. The nitrite scavenging abilities at pH 1.5, pH 3.0, pH 6.0 were 52.8%, 29.4%, 15.4%, respectively; these abilities decreased as pH increased. We conclude that Hominis Placenta Herbal Acupuncture may be useful as potential sources of antioxidant.

  13. Ureaplasma Urealyticum or Mycoplasma Hominis Infections and Semen Quality of Infertile Men in Abidjan

    Institute of Scientific and Technical Information of China (English)

    Zinzendorf NY; Kouassi-Agbessi BT; Lathro JS; Don C; Kouadio L; Loukou YG

    2008-01-01

    Objective To determine the prevalence of U.urealvticum and M.hominis in semen samples collected from men admitted in clinic for infertility,and to compare the quality of these semen samples.Methods A total of 1058 semen samples collected were investigated.Sperm semiological assays were performed according to the guidelines of the World Health Organisation(WHO).Semen were examined by Mycoplasma IST for the detection of mycoplasma.Semen culture on agar media was used to detect other microorganisms.Chlamydia was detected using direct fluorescent assay(DFA)of Clamydia Trachomatis.Results Among 1058 semen samples,microorganisms were detected in 638(60.3%).The infected sperms consisted of mycoplasma alone in 507 cases(47.9%).mycoplasma and other microorganisms in 98(9.3%),giving in all 605(57.2%)samples infected with mycoplasma.The last 33(3.1%)consisted of other microorganisms alone.The frequency of U.urealyticum.M.hominis and mixed genital infections detected in semen samples of infertile men were 39%,23.8% and 5.6%,respectively.The rates of abnormal semen parameters recorded among patients infected with mycoplasma were for volume(22.2%-25%),viscosity(29.6%-43.5%),pH(64.7%-72.9%),motility(80.8%-93.8%),morphology(36.3%-47.9%),sperm concentration(53.3%-58.3%)and leukocyte count(51.4%-58.3%).Conclusion Frequency of U.urealyticum infection was higher than that of M.hominis.Mycoplasma infections were associated with disorders of pH,motility and sperm concentration.In addition M.hominis infection affected spermatozoa morphology.Therefore,screening of U.urealyticum and M.hominis for routine semen analysis is clinically relevant in Abidjan.

  14. Activity of medicinal plants from Ghana against the parasitic gut protist Blastocystis

    DEFF Research Database (Denmark)

    Bremer Christensen, Charlotte; Soelberg, Jens; Stensvold, Christen R;

    2015-01-01

    with other flavourings. The aim of this study was to evaluate the anti-Blastocystis activity of 24 plant parts from 21 medicinal plants from Ghana. MATERIALS AND METHODS: The medicinal plants were collected in the Greater Accra region of Ghana. Every plant part was tested in three different extracts...... activity as the reference anti-protozoal drug MTZ. Historically, the active plants found in this study have been used against dysentery, diarrhoea or other stomach disorders. Nowadays they are not used specifically for dysentery, but they are being used as medicinal plants against various stomach disorders....

  15. Blastocystis is associated with decrease of fecal microbiota protective bacteria: comparative analysis between patients with irritable bowel syndrome and control subjects.

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    Céline Nourrisson

    Full Text Available Blastocystis is a protistan parasite living in the digestive tract of many animals, including humans. This highly prevalent intestinal parasite is suspected to be linked to Irritable Bowel Syndrome (IBS, a chronic functional bowel disorder. Here, we first compared the prevalence of Blastocystis among 56 IBS patients (40 IBS with constipation (IBS-C, 9 IBS with diarrhea (IBS-D, 4 mixed IBS (IBS-M and 3 unsubtyped IBS (IBS-U according to the Rome III criteria and 56 control (i.e. without any diagnosed chronic or acute gastrointestinal disorder subjects. The highest prevalence of Blastocystis spp. was observed in the IBS group, but was only statistically significant in men (36.8% in the IBS group versus 4.8% in the control group. We then conducted a meta-analysis including epidemiological studies attempting to determine whether Blastocystis carriage could be linked to IBS, and highlighted that IBS patients had a relative risk of 2.34 to be infected by Blastocystis when compared to non-IBS subjects. We also looked for Dientamoeba fragilis, which is often associated with IBS, and identified this parasite only in some IBS patients (n = 6/56. Several studies provided evidence for a major role of the gut microbiota in the pathophysiology of IBS. Thus, we investigated the possible impact of Blastocystis carriage on the enteric bacterial community through quantification of 8 major bacterial groups from the enteric flora. Our data indicated that men with IBS-C had a significant decrease in Bifidobacterium sp. when infected by Blastocystis. Interestingly, in control subjects (i.e. without any gastrointestinal disorder positive for Blastocystis, Faecalibacterium prausnitzii, which is known for its anti-inflammatory properties, was significantly decreased in men. Our results support the hypothesis that Blastocystis might be linked to the pathophysiology of IBS-C and intestinal flora imbalance.

  16. Blastocystis is associated with decrease of fecal microbiota protective bacteria: comparative analysis between patients with irritable bowel syndrome and control subjects.

    Science.gov (United States)

    Nourrisson, Céline; Scanzi, Julien; Pereira, Bruno; NkoudMongo, Christina; Wawrzyniak, Ivan; Cian, Amandine; Viscogliosi, Eric; Livrelli, Valérie; Delbac, Frédéric; Dapoigny, Michel; Poirier, Philippe

    2014-01-01

    Blastocystis is a protistan parasite living in the digestive tract of many animals, including humans. This highly prevalent intestinal parasite is suspected to be linked to Irritable Bowel Syndrome (IBS), a chronic functional bowel disorder. Here, we first compared the prevalence of Blastocystis among 56 IBS patients (40 IBS with constipation (IBS-C), 9 IBS with diarrhea (IBS-D), 4 mixed IBS (IBS-M) and 3 unsubtyped IBS (IBS-U) according to the Rome III criteria) and 56 control (i.e. without any diagnosed chronic or acute gastrointestinal disorder) subjects. The highest prevalence of Blastocystis spp. was observed in the IBS group, but was only statistically significant in men (36.8% in the IBS group versus 4.8% in the control group). We then conducted a meta-analysis including epidemiological studies attempting to determine whether Blastocystis carriage could be linked to IBS, and highlighted that IBS patients had a relative risk of 2.34 to be infected by Blastocystis when compared to non-IBS subjects. We also looked for Dientamoeba fragilis, which is often associated with IBS, and identified this parasite only in some IBS patients (n = 6/56). Several studies provided evidence for a major role of the gut microbiota in the pathophysiology of IBS. Thus, we investigated the possible impact of Blastocystis carriage on the enteric bacterial community through quantification of 8 major bacterial groups from the enteric flora. Our data indicated that men with IBS-C had a significant decrease in Bifidobacterium sp. when infected by Blastocystis. Interestingly, in control subjects (i.e. without any gastrointestinal disorder) positive for Blastocystis, Faecalibacterium prausnitzii, which is known for its anti-inflammatory properties, was significantly decreased in men. Our results support the hypothesis that Blastocystis might be linked to the pathophysiology of IBS-C and intestinal flora imbalance. PMID:25365580

  17. The Clinical Study on a Case of Transverse Myelitis With Bee Venom and Hominis Placenta Herbal Acupuncture

    OpenAIRE

    Park Min-ho; Lim Sung-taek; Choi Seok-woo

    2005-01-01

    Objective : This study was performed to evaluate the treatment of acupuncture therapy including Bee Venom and Hominis Placenta herbal acupuncture on the patient with Transverse myelitis. Methods : We treated the patient with Transverse myelitis by Bee Venom herbal acupuncture at beginning, since then we treated him adding to Hominis Placenta herbal acupuncture. Conclusions : The patient was effectively reduced symptoms with Bee Venom herbal acupuncture, since then he get more effective ...

  18. A Pilot Study on Single-dose Toxicity Testing of Hominis placenta Pharmacopuncture in Sprague-Dawley Rats

    OpenAIRE

    Yoo-Hwan Lee; Hyun-Min Yoon; Kyung-Jeon Jang; Cheol-Hong Kim

    2015-01-01

    Objectives: This study was performed to analyze the toxicity and to find the lethal dose of the test substance Hominis placenta pharmacopuncture when used as a single-dose in 6 week old, male and female Sprague-Dawley (SD) rats. Methods: All experiments were conducted at Biotoxtech (Chungwon, Korea), an institution authorized to perform non clinical studies, under the regulations of Good Laboratory Practice (GLP). SD rats were chosen for the pilot study. Doses of Hominis placenta pharmacopunc...

  19. Detection of concurrent infection of dairy cattle with Blastocystis, Cryptosporidium, Giardia, and Enterocytozoon by molecular and microscopic methods

    Science.gov (United States)

    Of fecal specimens examined from 47 dairy cattle ranging in age from neonates to multiparous cows, 9, 10, 24, and 17 were positive for Blastocystis spp., Cryptosporidium spp., Giardia duodenalis, and Enterocytozoon bieneusi, respectively, as determined by PCR. Eight 3- to 5-month-old cattle were co...

  20. PATHOGENICITY OF Blastocystis sp. TO THE GASTROINTESTINAL TRACT OF MICE: RELATIONSHIP BETWEEN INOCULUM SIZE AND PERIOD OF INFECTION.

    Science.gov (United States)

    Pavanelli, Mariana F; Kaneshima, Edilson Nobuyoshi; Uda, Carla F; Colli, Cristiane M; Falavigna-Guilherm, Ana L; Gomes, Mônica L

    2015-12-01

    The pathogenic potential of Blastocystis sp. in experimental models requires further investigation. In this work, the pathogenicity of this parasite in the gastrointestinal tract of male Swiss mice was evaluated according to the inoculum size and period of infection. Animals were infected intragastrically, with 100, 500, 1,000, 5,000 and 10,000 Blastocystis sp. vacuolar forms obtained from a mixture of eight human isolates cultured axenically in Jones' medium. After seven, 14, 21, 28 and 60 days of infection, the animals were sacrificed and fragments of the small intestine (duodenum), large intestine, and cecum were subjected to histopathological analysis. Blastocystis sp. triggered an inflammatory response in the different tissues analyzed, with a predominance of mononuclear cells. The parasite was found in the muscular layer of the cecum, showing its invasive character. Larger inocula triggered inflammatory processes earlier (seven days) than smaller ones (from 21 days). We conclude that, in the proposed model, the pathogenicity of Blastocystis sp. isolates that were studied is related to inoculum size and period of infection. PMID:27049699

  1. Detection of multiple zoonotic infectious agents in cattle with Blastocystis, Cryptosporidium, Giardia, and Enterocytozoon by molecular and microscopic methods

    Science.gov (United States)

    Feces from 47 dairy cattle ranging in age from neonates to multiparous cows were examined and 9, 10, 24, and 17 were found positive for Blastocystis spp., Cryptosporidium spp., Giardia duodenalis, and Enterocytozoon bieneusi, respectively, by PCR. Eight 3- to 5-month-old cattle were concurrently i...

  2. Symbiotic Association with Mycoplasma hominis Can Influence Growth Rate, ATP Production, Cytolysis and Inflammatory Response of Trichomonas vaginalis

    Science.gov (United States)

    Margarita, Valentina; Rappelli, Paola; Dessì, Daniele; Pintus, Gianfranco; Hirt, Robert P.; Fiori, Pier L.

    2016-01-01

    The symbiosis between the parasitic protist Trichomonas vaginalis and the opportunistic bacterium Mycoplasma hominis is the only one currently described involving two obligate human mucosal symbionts with pathogenic capabilities that can cause independent diseases in the same anatomical site: the lower urogenital tract. Although several aspects of this intriguing microbial partnership have been investigated, many questions on the influence of this symbiosis on the parasite pathobiology still remain unanswered. Here, we examined with in vitro cultures how M. hominis could influence the pathobiology of T. vaginalis by investigating the influence of M. hominis on parasite replication rate, haemolytic activity and ATP production. By comparing isogenic mycoplasma-free T. vaginalis and parasites stably associated with M. hominis we could demonstrate that the latter show a higher replication rate, increased haemolytic activity and are able to produce larger amounts of ATP. In addition, we demonstrated in a T. vaginalis-macrophage co-culture system that M. hominis could modulate an aspect of the innate immuno-response to T. vaginalis infections by influencing the production of nitric oxide (NO) by human macrophages, with the parasite-bacteria symbiosis outcompeting the human cells for the key substrate arginine. These results support a model in which the symbiosis between T. vaginalis and M. hominis influences host-microbes interactions to the benefit of both microbial partners during infections and to the detriment of their host. PMID:27379081

  3. Computational prediction of Mycoplasma hominis proteins targeting in nucleus of host cell and their implication in prostate cancer etiology.

    Science.gov (United States)

    Khan, Shahanavaj; Zakariah, Mohammed; Palaniappan, Sellappan

    2016-08-01

    Cancer has long been assumed to be a genetic disease. However, recent evidence supports the enigmatic connection of bacterial infection with the growth and development of various types of cancers. The cause and mechanism of the growth and development of prostate cancer due to Mycoplasma hominis remain unclear. Prostate cancer cells are infected and colonized by enteroinvasive M. hominis, which controls several factors that can affect prostate cancer growth in susceptible persons. We investigated M. hominis proteins targeting the nucleus of host cells and their implications in prostate cancer etiology. Many vital processes are controlled in the nucleus, where the proteins targeting M. hominis may have various potential implications. A total of 29/563 M. hominis proteins were predicted to target the nucleus of host cells. These include numerous proteins with the capability to alter normal growth activities. In conclusion, our results emphasize that various proteins of M. hominis targeted the nucleus of host cells and were involved in prostate cancer etiology through different mechanisms and strategies.

  4. Increase number of mitochondrion-like organelle in symptomatic Blastocystis subtype 3 due to metronidazole treatment.

    Science.gov (United States)

    Raman, Kalyani; Kumar, Suresh; Chye, Tan Tian

    2016-01-01

    Blastocystis sp., an intestinal organism is known to cause diarrhea with metronidazole regarded as the first line of treatment despite reports of its resistance. The conflicting reports of variation in drug treatment have been ascribed to subtype differences. The present study evaluated in vitro responses due to metronidazole on ST3 isolated from three symptomatic and asymptomatic patients, respectively. Symptomatic isolates were obtained from clinical patients who showed symptoms such as diarrhea and abdominal bloating. Asymptomatic isolates from a stool survey carried out in a rural area. These patients had no other pathogens other than Blastocystis. Ultrastructural studies using transmission electron microscopy (TEM) and scanning electron microscopy (SEM) revealed drug-treated ST3 from symptomatic patients were irregular and amoebic with surface showing high-convoluted folding when treated with metronidazole. These organisms had higher number of mitochondrion-like organelle (MLO) with prominent cristae. However, the drug-treated ST3 from asymptomatic persons remained spherical in shape. Asymptomatic ST3 showed increase in the size of its central body with the MLO located at the periphery. PMID:26481491

  5. Protein Coding Gene Nucleotide Substitution Pattern in the Apicomplexan Protozoa Cryptosporidium parvum and Cryptosporidium hominis

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    Guangtao Ge

    2008-01-01

    Full Text Available Cryptosporidium parvum and C. hominis are related protozoan pathogens which infect the intestinal epithelium of humans and other vertebrates. To explore the evolution of these parasites, and identify genes under positive selection, we performed a pairwise whole-genome comparison between all orthologous protein coding genes in C. parvum and C. hominis. Genome-wide calculation of the ratio of nonsynonymous versus synonymous nucleotide substitutions (dN/dS was performed to detect the impact of positive and purifying selection. Of 2465 pairs of orthologous genes, a total of 27 (1.1% showed a high ratio of nonsynonymous substitutions, consistent with positive selection. A majority of these genes were annotated as hypothetical proteins. In addition, proteins with transmembrane and signal peptide domains are significantly more frequent in the high dN/dS group.

  6. Prevalence of Mycoplasma genitalium, Mycoplasma hominis and Chlamydia trachomatis among Danish patients requesting abortion

    DEFF Research Database (Denmark)

    Baczynska, Agata; Hvid, Malene; Lamy, P;

    2008-01-01

    The aim of the study was to determine lower genital tract carriage rate of Mycoplasma genitalium (M. genitalium) and to compare it to the carriage rates of Mycoplasma hominis (M. hominis ) and Chlamydia trachomatis (C. trachomatis) among 102 women requesting termination of pregnancy at the Horsens...... Hospital in Denmark. Real-Time PCR was used for the detection of bacterial DNA, and the presence of antibodies to the three microorganisms was determined by ELISA and immunoblotting. Real-Time PCR detected M. genitalium in one swab sample (0.98%) only, while the prevalence of C. trachomatis was high (15....... There was no correlation between the presence of genital infection with C. trachomatis and genital mycoplasmas and no correlation between the presence of antibodies to these bacteria. In conclusion, in Danish patients it is not necessary to test for M. genitalium before abortion since less than 1% were found positive...

  7. Ex Vivo and In Vivo Mice Models to Study Blastocystis spp. Adhesion, Colonization and Pathology: Closer to Proving Koch's Postulates.

    Science.gov (United States)

    Ajjampur, Sitara S R; Png, Chin Wen; Chia, Wan Ni; Zhang, Yongliang; Tan, Kevin S W

    2016-01-01

    Blastocystis spp. are widely prevalent extra cellular, non-motile anerobic protists that inhabit the gastrointestinal tract. Although Blastocystis spp. have been associated with gastrointestinal symptoms, irritable bowel syndrome and urticaria, their clinical significance has remained controversial. We established an ex vivo mouse explant model to characterize adhesion in the context of tissue architecture and presence of the mucin layer. Using confocal microscopy with tissue whole mounts and two axenic isolates of Blastocystis spp., subtype 7 with notable differences in adhesion to intestinal epithelial cells (IEC), isolate B (ST7-B) and isolate H (more adhesive, ST7-H), we showed that adhesion is both isolate dependent and tissue trophic. The more adhesive isolate, ST7-H was found to bind preferentially to the colon tissue than caecum and terminal ileum. Both isolates were also found to have mucinolytic effects. We then adapted a DSS colitis mouse model as a susceptible model to study colonization and acute infection by intra-caecal inoculation of trophic Blastocystis spp.cells. We found that the more adhesive isolate ST7-H was also a better colonizer with more mice shedding parasites and for a longer duration than ST7-B. Adhesion and colonization was also associated with increased virulence as ST7-H infected mice showed greater tissue damage than ST7-B. Both the ex vivo and in vivo models used in this study showed that Blastocystis spp. remain luminal and predominantly associated with mucin. This was further confirmed using colonic loop experiments. We were also successfully able to re-infect a second batch of mice with ST7-H isolates obtained from fecal cultures and demonstrated similar histopathological findings and tissue damage thereby coming closer to proving Koch's postulates for this parasite. PMID:27508942

  8. Ex Vivo and In Vivo Mice Models to Study Blastocystis spp. Adhesion, Colonization and Pathology: Closer to Proving Koch's Postulates

    Science.gov (United States)

    Ajjampur, Sitara S. R.; Png, Chin Wen; Chia, Wan Ni; Zhang, Yongliang; Tan, Kevin S. W.

    2016-01-01

    Blastocystis spp. are widely prevalent extra cellular, non-motile anerobic protists that inhabit the gastrointestinal tract. Although Blastocystis spp. have been associated with gastrointestinal symptoms, irritable bowel syndrome and urticaria, their clinical significance has remained controversial. We established an ex vivo mouse explant model to characterize adhesion in the context of tissue architecture and presence of the mucin layer. Using confocal microscopy with tissue whole mounts and two axenic isolates of Blastocystis spp., subtype 7 with notable differences in adhesion to intestinal epithelial cells (IEC), isolate B (ST7-B) and isolate H (more adhesive, ST7-H), we showed that adhesion is both isolate dependent and tissue trophic. The more adhesive isolate, ST7-H was found to bind preferentially to the colon tissue than caecum and terminal ileum. Both isolates were also found to have mucinolytic effects. We then adapted a DSS colitis mouse model as a susceptible model to study colonization and acute infection by intra-caecal inoculation of trophic Blastocystis spp.cells. We found that the more adhesive isolate ST7-H was also a better colonizer with more mice shedding parasites and for a longer duration than ST7-B. Adhesion and colonization was also associated with increased virulence as ST7-H infected mice showed greater tissue damage than ST7-B. Both the ex vivo and in vivo models used in this study showed that Blastocystis spp. remain luminal and predominantly associated with mucin. This was further confirmed using colonic loop experiments. We were also successfully able to re-infect a second batch of mice with ST7-H isolates obtained from fecal cultures and demonstrated similar histopathological findings and tissue damage thereby coming closer to proving Koch’s postulates for this parasite. PMID:27508942

  9. Susceptibilities of Mycoplasma hominis, Mycoplasma pneumoniae, and Ureaplasma urealyticum to a new quinolone, OPC 17116.

    OpenAIRE

    Kenny, G. E.; Cartwright, F D

    1993-01-01

    The susceptibilities of human mycoplasmas to OPC 17116 (Otsuka America Pharmaceutical, Inc., Rockville, Md.) and temafloxacin (Abbott Laboratories, Chicago, Ill.) were determined by the agar dilution method and were compared with those to sparfloxacin and ofloxacin. The MICs of OPC 17116 for 90% of Mycoplasma pneumoniae (0.25 microgram/ml) and Mycoplasma hominis (0.125 micrograms/ml) isolates tested were closely similar to those of sparfloxacin and were four- to eightfold greater than those o...

  10. Comparison of methods for detection of Blastocystis infection in routinely submitted stool samples, and also in IBS/IBD Patients in Ankara, Turkey.

    Directory of Open Access Journals (Sweden)

    Funda Dogruman-Al

    Full Text Available BACKGROUND: This study compared diagnostic methods for identifying Blastocystis in stool samples, and evaluated the frequency of detection of Blastocystis in patients with irritable bowel syndrome (IBS and inflammatory bowel disease (IBD. RESULTS AND DISCUSSION: From a set of 105 stool specimens submitted for routine parasitological analysis, 30 were identified as positive for Blastocystis by the culture method. From that group of 30 positives, Lugol's stain, trichrome staining, and an immunofluorescence assay identified 11, 15, and 26 samples as positive respectively. Using culture as a standard, the sensitivity of Lugol's stain was 36.7%, trichrome staining was 50%, and the IFA stain was 86.7%. The specificity of Lugol's stain was 91%, trichrome staining was 100%, and the IFA stain was 97.3%. In the group of 27 IBS and IBD patients, using all methods combined, we detected Blastocystis in 67% (18/27 of the patients. Blastocystis was detected in 33% (2/6 of IBD patients and 76% (16/21 of IBS patients. For comparison, trichrome staining alone, the method most frequently used in many countries, would have only identified Blastocystis infection in 29% (6/21 of the IBS patients. No parasitic co-infections were identified in the IBS/IBD patients. Most Blastocystis-positive IBS/IBD patients were over 36 with an average length of illness of 4.9 years. CONCLUSIONS: Most IBS patients in this study were infected with Blastocystis. IFA staining may be a useful alternative to stool culture, especially if stool specimens have been chemically preserved.

  11. Life on arginine for Mycoplasma hominis: clues from its minimal genome and comparison with other human urogenital mycoplasmas.

    Directory of Open Access Journals (Sweden)

    Sabine Pereyre

    2009-10-01

    Full Text Available Mycoplasma hominis is an opportunistic human mycoplasma. Two other pathogenic human species, M. genitalium and Ureaplasma parvum, reside within the same natural niche as M. hominis: the urogenital tract. These three species have overlapping, but distinct, pathogenic roles. They have minimal genomes and, thus, reduced metabolic capabilities characterized by distinct energy-generating pathways. Analysis of the M. hominis PG21 genome sequence revealed that it is the second smallest genome among self-replicating free living organisms (665,445 bp, 537 coding sequences (CDSs. Five clusters of genes were predicted to have undergone horizontal gene transfer (HGT between M. hominis and the phylogenetically distant U. parvum species. We reconstructed M. hominis metabolic pathways from the predicted genes, with particular emphasis on energy-generating pathways. The Embden-Meyerhoff-Parnas pathway was incomplete, with a single enzyme absent. We identified the three proteins constituting the arginine dihydrolase pathway. This pathway was found essential to promote growth in vivo. The predicted presence of dimethylarginine dimethylaminohydrolase suggested that arginine catabolism is more complex than initially described. This enzyme may have been acquired by HGT from non-mollicute bacteria. Comparison of the three minimal mollicute genomes showed that 247 CDSs were common to all three genomes, whereas 220 CDSs were specific to M. hominis, 172 CDSs were specific to M. genitalium, and 280 CDSs were specific to U. parvum. Within these species-specific genes, two major sets of genes could be identified: one including genes involved in various energy-generating pathways, depending on the energy source used (glucose, urea, or arginine and another involved in cytadherence and virulence. Therefore, a minimal mycoplasma cell, not including cytadherence and virulence-related genes, could be envisaged containing a core genome (247 genes, plus a set of genes required for

  12. High rates of double-stranded RNA viruses and Mycoplasma hominis in Trichomonas vaginalis clinical isolates in South Brazil.

    Science.gov (United States)

    da Luz Becker, Débora; dos Santos, Odelta; Frasson, Amanda Piccoli; de Vargas Rigo, Graziela; Macedo, Alexandre José; Tasca, Tiana

    2015-08-01

    Trichomonas vaginalis is the etiological agent of trichomoniasis, the most common non-viral sexually transmitted disease (STD) in world, with 276.4 million new cases each year. T. vaginalis can be naturally infected with Mycoplasma hominis and Trichomonasvirus species. This study aimed to evaluate the prevalence of T. vaginalis infected with four distinct T. vaginalis viruses (TVVs) and M. hominis among isolates from patients in Porto Alegre city, South Brazil. An additional goal of this study was to investigate whether there is association between metronidazole resistance and the presence of M. hominis during TVV infection. The RNA expression level of the pyruvate ferredoxin oxidoreductase (PFOR) gene was also evaluated among metronidazole-resistant and metronidazole-sensitive T. vaginalis isolates. A total of 530 urine samples were evaluated, and 5.7% samples were positive for T. vaginalis infection. Among them, 4.51% were isolated from female patients and 1.12% were from male patients. Remarkably, the prevalence rates of M. hominis and TVV-positive T. vaginalis isolates were 56.7% and 90%, respectively. Most of the T. vaginalis isolates were metronidazole-sensitive (86.7%), and only four isolates (13.3%) were resistant. There is no statistically significant association between infection by M. hominis and infection by TVVs. Our results refute the hypothesis that the presence of the M. hominis and TVVs is enough to confer metronidazole resistance to T. vaginalis isolates. Additionally, the role of PFOR RNA expression levels in metronidazole resistance as the main mechanism of resistance to metronidazole could not be established. This study is the first report of the T. vaginalis infection by M. hominis and TVVs in a large collection of isolates from South Brazil.

  13. High rates of double-stranded RNA viruses and Mycoplasma hominis in Trichomonas vaginalis clinical isolates in South Brazil.

    Science.gov (United States)

    da Luz Becker, Débora; dos Santos, Odelta; Frasson, Amanda Piccoli; de Vargas Rigo, Graziela; Macedo, Alexandre José; Tasca, Tiana

    2015-08-01

    Trichomonas vaginalis is the etiological agent of trichomoniasis, the most common non-viral sexually transmitted disease (STD) in world, with 276.4 million new cases each year. T. vaginalis can be naturally infected with Mycoplasma hominis and Trichomonasvirus species. This study aimed to evaluate the prevalence of T. vaginalis infected with four distinct T. vaginalis viruses (TVVs) and M. hominis among isolates from patients in Porto Alegre city, South Brazil. An additional goal of this study was to investigate whether there is association between metronidazole resistance and the presence of M. hominis during TVV infection. The RNA expression level of the pyruvate ferredoxin oxidoreductase (PFOR) gene was also evaluated among metronidazole-resistant and metronidazole-sensitive T. vaginalis isolates. A total of 530 urine samples were evaluated, and 5.7% samples were positive for T. vaginalis infection. Among them, 4.51% were isolated from female patients and 1.12% were from male patients. Remarkably, the prevalence rates of M. hominis and TVV-positive T. vaginalis isolates were 56.7% and 90%, respectively. Most of the T. vaginalis isolates were metronidazole-sensitive (86.7%), and only four isolates (13.3%) were resistant. There is no statistically significant association between infection by M. hominis and infection by TVVs. Our results refute the hypothesis that the presence of the M. hominis and TVVs is enough to confer metronidazole resistance to T. vaginalis isolates. Additionally, the role of PFOR RNA expression levels in metronidazole resistance as the main mechanism of resistance to metronidazole could not be established. This study is the first report of the T. vaginalis infection by M. hominis and TVVs in a large collection of isolates from South Brazil. PMID:26160539

  14. In vivo antiprotozoan effects of garlic (Allium sativum) and ginger (Zingiber officinale) extracts on experimentally infected mice with Blastocystis spp.

    Science.gov (United States)

    Abdel-Hafeez, Ekhlas H; Ahmad, Azza K; Kamal, Amany M; Abdellatif, Manal Z M; Abdelgelil, Noha H

    2015-09-01

    Controversy surrounding the pathogenic role of Blastocystis spp. in humans and lack of well-established diagnostic criteria led to debates concerning the treatment for that organism. Furthermore, some strains develop resistance against the recommended drugs. Thus, using natural medicine has many positive aspects to address these points. In an earlier study, we addressed in vitro effect of garlic and ginger on Blastocystis spp. isolates as an alternative treatment. Accordingly, this study was conducted to evaluate in vivo activities of these two herbs on mice infected with Blastocystis spp. Antiprotozoan activities were determined by monitoring Blastocystis shedding in stools and histopathological changes of the intestine of infected mice. Additionally, assessment of the antioxidant effect (via measuring the level of malondialdehyde (MDA) production) of these herbs on the treated groups of mice was done. Also, their effects on nitric oxide (NO) production were assessed. In this work, treatment of infected mice with garlic, ginger, and nitazoxanide (NTZ) reduced the shedding of cysts significantly compared to the infected untreated group, P value ≤0.001, 0.0001, and 0.0003, respectively. As well, histopathological examination revealed that Blastocystis was frequently observed within the lumen, at the tip of the epithelium, and/ or infiltrated in an enterocyte in the infected group without treatment compared to that of the infected treated ones. Furthermore, mice infected with Blastocystis exhibited increased levels of NO (440.09 ± 3.7 vs. 276.66 ± 0.8, P ≤ 0.001) and MDA production (106.19 ± 0.43 vs. 63.06 ± 0.45, P ≤ 0.0004) compared to that of the uninfected controls. Treatment of infected mice with garlic, ginger, and NTZ reduced NO levels to 54.41 ± 1.2, 47.70 ± 1.2, and 37.43 ± 0.98 and MDA levels to 22.38 ± 0.17, 63.34 ± 3.89, and 66.76 ± 9.1, respectively. We conclude that using ginger and garlic for treatment of blastocystosis is beneficial

  15. Genome Anatomy of Pyrenochaeta unguis-hominis UM 256, a Multidrug Resistant Strain Isolated from Skin Scraping

    Science.gov (United States)

    Toh, Yue Fen; Yew, Su Mei; Chan, Chai Ling; Na, Shiang Ling; Lee, Kok Wei; Hoh, Chee-Choong; Yee, Wai-Yan; Ng, Kee Peng

    2016-01-01

    Pyrenochaeta unguis-hominis is a rare human pathogen that causes infection in human skin and nail. P. unguis-hominis has received little attention, and thus, the basic biology and pathogenicity of this fungus is not fully understood. In this study, we performed in-depth analysis of the P. unguis-hominis UM 256 genome that was isolated from the skin scraping of a dermatitis patient. The isolate was identified to species level using a comprehensive multilocus phylogenetic analysis of the genus Pyrenochaeta. The assembled UM 256 genome has a size of 35.5 Mb and encodes 12,545 putative genes, and 0.34% of the assembled genome is predicted transposable elements. Its genomic features propose that the fungus is a heterothallic fungus that encodes a wide array of plant cell wall degrading enzymes, peptidases, and secondary metabolite biosynthetic enzymes. Antifungal drug resistance genes including MDR, CDR, and ERG11/CYP51 were identified in P. unguis-hominis UM 256, which may confer resistance to this fungus. The genome analysis of P. unguis-hominis provides an insight into molecular and genetic basis of the fungal lifestyles, understanding the unrevealed biology of antifungal resistance in this fungus. PMID:27626635

  16. Effect of a Mycoplasma hominis-Like Mycoplasma on the Infection of HEp-2 Cells by the TW-183 Strain of Chlamydia pneumoniae

    OpenAIRE

    Castilla, Elias A.; Wadowsky, Robert M.

    2000-01-01

    We isolated a Mycoplasma hominis-like mycoplasma from a stock culture of Chlamydia pneumoniae TW-183 obtained from the American Type Culture Collection and eradicated the contaminant by treating the stock suspension with a nonionic detergent, Igepal CA-630. The M. hominis-like mycoplasma neither inhibits nor enhances the infectivity of C. pneumoniae for HEp-2 cells.

  17. Genetic Passive Immunization with Adenoviral Vector Expressing Chimeric Nanobody-Fc Molecules as Therapy for Genital Infection Caused by Mycoplasma hominis.

    Science.gov (United States)

    Burmistrova, Daria A; Tillib, Sergey V; Shcheblyakov, Dmitry V; Dolzhikova, Inna V; Shcherbinin, Dmitry N; Zubkova, Olga V; Ivanova, Tatiana I; Tukhvatulin, Amir I; Shmarov, Maxim M; Logunov, Denis Y; Naroditsky, Boris S; Gintsburg, Aleksandr L

    2016-01-01

    Developing pathogen-specific recombinant antibody fragments (especially nanobodies) is a very promising strategy for the treatment of infectious disease. Nanobodies have great potential for gene therapy application due to their single-gene nature. Historically, Mycoplasma hominis has not been considered pathogenic bacteria due to the lack of acute infection and partially due to multiple studies demonstrating high frequency of isolation of M. hominis samples from asymptomatic patients. However, recent studies on the role of latent M. hominis infection in oncologic transformation, especially prostate cancer, and reports that M. hominis infects Trichomonas and confers antibiotic resistance to Trichomonas, have generated new interest in this field. In the present study we have generated specific nanobody against M. hominis (aMh), for which the identified target is the ABC-transporter substrate-binding protein. aMh exhibits specific antibacterial action against M. hominis. In an attempt to improve the therapeutic properties, we have developed the adenoviral vector-based gene therapy approach for passive immunization with nanobodies against M. hominis. For better penetration into the mucous layer of the genital tract, we fused aMh with the Fc-fragment of IgG. Application of this comprehensive approach with a single systemic administration of recombinant adenovirus expressing aMh-Fc demonstrated both prophylactic and therapeutic effects in a mouse model of genital M. hominis infection. PMID:26962869

  18. Genetic Passive Immunization with Adenoviral Vector Expressing Chimeric Nanobody-Fc Molecules as Therapy for Genital Infection Caused by Mycoplasma hominis.

    Directory of Open Access Journals (Sweden)

    Daria A Burmistrova

    Full Text Available Developing pathogen-specific recombinant antibody fragments (especially nanobodies is a very promising strategy for the treatment of infectious disease. Nanobodies have great potential for gene therapy application due to their single-gene nature. Historically, Mycoplasma hominis has not been considered pathogenic bacteria due to the lack of acute infection and partially due to multiple studies demonstrating high frequency of isolation of M. hominis samples from asymptomatic patients. However, recent studies on the role of latent M. hominis infection in oncologic transformation, especially prostate cancer, and reports that M. hominis infects Trichomonas and confers antibiotic resistance to Trichomonas, have generated new interest in this field. In the present study we have generated specific nanobody against M. hominis (aMh, for which the identified target is the ABC-transporter substrate-binding protein. aMh exhibits specific antibacterial action against M. hominis. In an attempt to improve the therapeutic properties, we have developed the adenoviral vector-based gene therapy approach for passive immunization with nanobodies against M. hominis. For better penetration into the mucous layer of the genital tract, we fused aMh with the Fc-fragment of IgG. Application of this comprehensive approach with a single systemic administration of recombinant adenovirus expressing aMh-Fc demonstrated both prophylactic and therapeutic effects in a mouse model of genital M. hominis infection.

  19. Subtype analysis of Blastocystis sp. isolates from human and canine hosts in an urban community in the Philippines.

    Science.gov (United States)

    Belleza, Maria Luz B; Reyes, John Carlo B; Tongol-Rivera, Pilarita N; Rivera, Windell L

    2016-06-01

    Blastocystis sp. is a common gut-dwelling protist of both humans and animals. A cross-sectional survey among humans and their dogs was conducted to determine the prevalence of Blastocystis infection and to characterize the subtype (ST) distribution in an urban community in the Philippines. Fecal specimens from 1,271 humans and 145 dogs were collected and inoculated in diphasic culture medium. Prevalence of Blastocystis by culture was 13.0% (95% CI = 11.2-15.0) and 14.5% (95% CI = 9.6-21.2) for humans and dogs, respectively. A total of 168 culture isolates were genotyped using polymerase chain reaction (PCR) with seven pairs of ST-specific sequence-tagged-site (STS) primers. In humans, the ST present in this study were ST1 with 22.6% (95% CI = 17.2-29.0), ST2 with 3.1% (95% CI = 1.3-6.7), ST3 with 41.4% (95% CI= 34.9-48.6), ST4 with 14.8% (95% CI = 10.5-20.6), ST5 with 4.1% (95% CI = 2.0-8.0), and unknown ST with 13.9% (95% CI = 9.6-19.4). In dogs, the ST present in this study were ST1 with 4.3% (95% CI = 0.0-29.0), ST2 with 8.7% (95% CI = 1.3-28.0), ST3 with 17.4% (95% CI = 6.4-37.7), ST4 with 13.0% (95% CI = 3.7-33.0), ST5 with 13.0% (95% CI = 3.7-33.0), and unknown ST with 47.8% (95% CI = 29.2-67.0). This is the first study that reported Blastocystis ST4 in human and canine hosts in the Philippines. PMID:26902433

  20. Blastocystis infection in Malaysia: Evidence of waterborne and human-to-human transmissions among the Proto-Malay, Negrito and Senoi tribes of Orang Asli

    Directory of Open Access Journals (Sweden)

    Anuar Tengku Shahrul

    2013-02-01

    Full Text Available Abstract Background Blastocystis has been described as the most common intestinal parasite in humans and has an increased impact on public health. However, the transmission of this parasite has not been conclusively determined. Methods To contribute to a better comprehension of the epidemiology of this infection, a cross-sectional survey aimed at providing the first documented data on the prevalence and risk factors associated with Blastocystis infection was carried out among three Orang Asli tribes (Proto-Malay, Negrito and Senoi in selected villages at Negeri Sembilan, Perak and Pahang, Peninsular Malaysia. Faecal samples were examined by formalin-ether sedimentation and trichrome staining techniques. Results Of 500 individuals, 20.4% (102 were detected positive for Blastocystis; 13.3% (20/150 of Proto-Malays, 21.6% (30/139 of Negritos and 24.7% (52/211 of Senois were positive for Blastocystis, respectively. The positive cases showed a decrease with increasing age and most of the positive cases were observed in individuals less than 15 years old. Multivariate analysis confirmed that drinking untreated water and the presence of other family members infected with Blastocystis were significant risk factors of infection among the three tribes and overall population studied. Conclusion Essentially, the findings highlighted that Blastocystis infection is prevalent among Orang Asli communities in Malaysia. Further studies using molecular approaches to distinguish the subtype of Blastocystis is needed. The present study also revealed that this infection may be transmitted through waterborne and human-to-human contact. Therefore, interventions with the provision of clean water supply for the communities and health education especially to the parents are urgently required.

  1. THE METHODS OF LABORATORY DIAGNOSTICS OF UROGENITAL INFECTIONS ASSOCIATED WITH MYCOPLASMA HOMINIS AND UREAPLASMA SPP.

    Directory of Open Access Journals (Sweden)

    O. V. Zarucheynova

    2014-01-01

    Full Text Available Wide distribution of urogenital mycoplasmas in the population, the high frequency of carrier state and a long asymptomatic course of disease, the lack of specific clinical symptoms making the diagnosis impossible without using of special laboratory tests. The review focuses on indications for mycoplasma infection screening and for an appointmentof antibiotic therapy. The most commonly used laboratory diagnostic methods of urogenital infections, associated with Mycoplasma hominis and Ureaplasma spp., with their characteristics, advantages and disadvantages are described.

  2. Ultrastructural pathologic changes of Intestinal mucosa of mouse infected artificially by Blastocystis Hominis%人芽囊原虫感染小鼠肠黏膜超微病理变化观察

    Institute of Scientific and Technical Information of China (English)

    王东; 汤黎明; 李文; 苏云普; 张红卫; 薛长贵

    2005-01-01

    目的探讨人芽囊原虫人工感染后对肠黏膜的损伤情况.方法将昆明小鼠60只随机分为5组,一组为空白对照,其它四组分别经口感染104、204、304、404剂量的虫体.7天后处死动物,取回盲部,用电镜观察肠黏膜超微结构的改变.结果扫描电镜和透射电镜下观察,空白对照组小鼠未发现虫体寄生,肠黏膜结构正常.感染104、204剂量的小鼠有少量的虫体寄生,黏膜上皮细胞破损,部分微绒毛脱落,线粒体局部水肿,嵴排列紊乱.感染304、404剂量的小鼠可见有大量虫体侵入肠黏膜,黏膜上皮细胞破损严重,呈网状,蜂窝状,并有成片的肠黏膜脱落.线粒体膜开始溶解,嵴断裂、扭曲、黏连,呈空泡样变,粗面内质网囊泡变,黏膜表面细胞间的紧密连接融合.结论人芽囊原虫对回盲部肠黏膜有损伤作用,且与感染剂量有关,随感染剂量的增加病理变化越明显.

  3. Dot Enzyme-Linked Immunosorbent Assay for Detection of Serum Antibody to Blastocystis hominis in Humans%Dot-ELISA检测人芽囊原虫血清抗体的研究

    Institute of Scientific and Technical Information of China (English)

    苏水莲; 兰海英; 谢瑞莲; 黄真; 严宜明; 廖华; 陈桂凤; 张瑞其; 谢琼君; 黎晓; 胡雅琼; 曾雪英

    2007-01-01

    分别收集本院大学生322份血清和粪便,将粪便用体外培养法作为金标准检测出人芽囊原虫阳性178例,阴性144例.用dot-ELISA分别对体外培养法阳性者和阴性者血清进行检测,敏感度为92.13%(164/178),特异度为97.92%(141/144).表明dot-ELISA用于检测人芽囊原虫血清抗体较敏感、特异.

  4. 石榴根皮等9种中药体外抗人芽囊原虫的效果观察%The Trichomnacidal Effect of Nine Chinese Herbs on the Blastocystis Hominis

    Institute of Scientific and Technical Information of China (English)

    苏水莲; 陈桂凤; 张瑞其; 谢琼珺; 廖华

    2007-01-01

    目的 探讨石榴根皮等9种中草药杀人芽囊原虫的效果.方法 采用不同浓度中草药水提液进行体外抗人芽囊原虫实验,并用光镜观察作用后人芽囊原虫的形态变化.结果 随着药物作用时间的延长和药物浓度的增加,石榴根皮、胡黄连、黄连、苦楝皮对人芽囊原虫有不同程度的凋亡现象,这些药物对人芽囊原虫的MIC值分别为0.1,0.6,0.8,0.8 g/ml,光镜观察虫体表膜凹陷,剥脱,破裂.低浓度胡黄连药物作用虫体后均转变成包囊,而白头翁、南瓜子、使君子、槟榔、苦参对人芽囊原虫均无作用.结论 石榴根皮、胡黄连可直接作用于虫体表膜,具有较强的杀人芽囊原虫的作用.

  5. PCR-based Genotype Classification of Blastocystis hominis Isolates from College Students of Guangxi%广西在校大学生人芽囊原虫分离株基因型的初步研究

    Institute of Scientific and Technical Information of China (English)

    战廷正; 刘腾; 石焕焕; 何姗姗; 燕慧; 刘登宇

    2014-01-01

    为了解广西在校大学生人芽囊原虫分离株基因型构成,于2012年收集53例广西医科大学人芽囊原虫感染者粪便,分离虫体,经体外培养后抽提基因组DNA,用已知的7对STS引物进行PCR扩增.结果显示,53株人芽囊原虫分离株中共发现5种已知基因型.其中,1型(351 bp)4株,占7.6%; 3型(526 bp) 17株,占32.1%;4型(338 bp)4株,占7.6%; 6型(317 bp)1株,占1.9%;7型(487 bp)5株,占9.4%.未见基因2型、5型和混合基因型.22株无特异性条带出现,初步认定为未知基因型.

  6. 湘西自治州不同人群人芽囊原虫感染情况调查%Investigation on the infection of Blastocystis hominis in part of the populations in Xiangxi Autonomous Prefecture , Hunan

    Institute of Scientific and Technical Information of China (English)

    张开仁; 杨胜元; 彭海燕; 段绩辉

    2013-01-01

    目的 了解湘西自治州农村人群和腹痛、腹泻患者人芽囊原虫的感染情况.方法 对湘西凤凰县农村人群和在湘西自治州疾控中心寄生虫病室就诊的腹痛、腹泻患者收集便样,采用碘液涂片检查人芽囊原虫.结果 共检查1783人,人芽囊原虫阳性328人,阳性率为18.45%.其中农村人群粪检11个村1 439人,人芽囊原虫阳性226人,阳性率为15.71%;门诊腹痛、腹泻患者粪检344人,人芽囊原虫阳性102人,阳性率为29.65%,2者差异有统计学意义(x2=35.97,P<0.05);男女间感染率差异无统计学意义(x2=0.001,P>0.05);农村人群以青壮年为高,感染率为22.16%,而门诊患者以>5~ 10岁儿童为高,感染率为43.00%;土家族、苗族感染率分别20.67%、19.11%,2者无统计学意义,而汉族低于前2者为13.79%,差异有统计学意义(x2=8.91 P<0.05);在学龄前儿童、学生、职员、农民人群中,以农民为高,感染率为21.19%;城、乡人群感染率分别为15.71%和19.35%,差异无统计学意义(x2=3.07,P>0.05).湘西自治州农村人群感染率最低的村为7.35%,最高的村为33.33%.结论 湘西自治州人群人芽囊原虫感染率较高,人芽囊原虫是腹痛、腹泻患者的主要病因之一.

  7. 人芽囊原虫对实验感染昆明小鼠肠黏膜超微结构的影响%Impact of Blastocystis hominis Infection on Ultrastructure of Intestinal Mucosa in Mice

    Institute of Scientific and Technical Information of China (English)

    张红卫; 李文; 颜秋叶; 贺丽君; 苏云普

    2006-01-01

    目的观察昆明小鼠感染人芽囊原虫后肠黏膜超微结构的改变,探讨人芽囊原虫的致病机制.方法20只昆明小鼠随机分为4组:A和D组为接受免疫抑制剂(地塞米松)处理,B组不用地塞米松处理,C组为正常对照组.A组和B组经口感染204人芽囊原虫包囊等,C组和D组灌注等量Locke氏液作为对照.感染6 d后,剖杀各组小鼠取回盲部肠黏膜处理后,扫描和透射电镜下观察其超微结构.结果扫描电镜下见A、B两组人芽囊原虫寄生在小鼠回盲部肠腔和肠黏膜表面,个别虫体入侵肠黏膜及肠黏膜皱襞,部分肠黏膜微绒毛呈局灶性破坏;透射电镜下见部分吸收细胞表面微绒毛数目减少,吸收细胞和杯状细胞线粒体水肿,粗面内质网扩张、脱颗粒,间质内淋巴细胞浸润及嗜酸粒细胞增多.A组病变程度比B组重,C组和D组未见异常.结论感染人芽囊原虫的昆明小鼠回盲部肠黏膜超微结构有严重的损害,肠黏膜损伤程度受机体免疫状态的影响.

  8. Pathological changes of intestinal mucosa in Kunming mice infected Blastocystis hominis%感染人芽囊原虫昆明鼠肠粘膜的病理改变

    Institute of Scientific and Technical Information of China (English)

    张红卫; 贺丽君; 颜秋叶; 苏云普; 李文; 薛长贵; 王东

    2006-01-01

    目的通过观察昆明鼠感染人芽囊原虫后肠粘膜病理变化,探讨人芽囊原虫的致病机制. 方法从腹泻患者粪便中分离培养人芽囊原虫.15只昆明鼠分为3组:A组为接受免疫抑制剂处理组,B组为非免疫抑制剂处理组,经口感染人芽囊原虫1.6×105个/鼠,观察小鼠感染情况及肠粘膜的病理变化;C组为健康对照组. 结果实验鼠人芽囊原虫检出率90%(9/10),虫体主要寄生在小鼠回盲部肠腔和肠粘膜表面,个别虫体侵入肠粘膜上皮,部分肠粘膜充血水肿,腺体增生,粘液分泌增强,可见有炎症细胞浸润,偶见局灶性肠粘膜坏死.病变程度免疫抑制剂处理组比未接种免疫抑制剂处理组重. 结论感染人芽囊原虫的昆明鼠回盲部肠粘膜发生病理损害,病变程度受宿主机体免疫状况的影响.

  9. 人芽囊原虫的致病性与遗传多样性研究进展%Research progress on pathogenicity and genetic diversity of Blastocystis hominis

    Institute of Scientific and Technical Information of China (English)

    李兰花; 周晓农

    2007-01-01

    人芽囊原虫是否具有致病性一直颇有争议,无论是流行病学研究还是实验动物研究均存在相互矛盾的结论.研究发现,人芽囊原虫具有广泛的遗传多样性,近年来,研究者用各种方法对此进行了研究.他们将人芽囊原虫分成不同的种群,并尝试探讨其遗传多样性与致病性的关系.该文就人芽囊原虫的致病性、遗传多样性及两者关系的研究进展进行了综述.

  10. 人芽囊原虫患者肠道SIgA的检测及其临床意义%Detection and its significance of secretory immunoglobulin A in the intestinal mucosa and feces from patients with Blastocystis hominis infection

    Institute of Scientific and Technical Information of China (English)

    金群馨; 唐连凤; 田春林; 卢作超; 俞开敏

    2005-01-01

    目的探讨人芽囊原虫感染者肠粘膜SIgA与粪便SIgA的水平,并分析与电镜下肠粘膜损伤程度的关系. 方法人芽囊原虫经粪便检查诊断的感染组30例和正常对照组30例,均经电子肠镜检查肠粘膜情况,并于镜下取肠粘膜组织送病理检查.另取肠粘膜组织与粪便,用放免法检测SIgA的水平. 结果肠镜检查可见感染者的肠粘膜有不同程度的糜烂及溃疡,病理多为粘膜慢性炎症及溃疡.感染组SIgA明显高于对照组,升高的程度与感染的程度正相关( rs = 0.4154, P<0.05),肠粘膜损伤的程度与感染的程度亦正相关(rs = 0.8538,P<0.001). 结论在发病期间,感染者肠粘膜及粪便SIgA升高与感染的程度相关,而粘膜损伤程度也和感染的程度相关,提示SIgA在粘膜免疫中起着重要的作用.人芽囊原虫感染所致肠粘膜损伤,可能与原虫的侵入及粘膜的免疫反应有关.

  11. 广西人芽囊原虫分离株同工酶谱的初步分析%Isoenzyme Patterns of the Isolates of Blastocystis hominis from Guangxi

    Institute of Scientific and Technical Information of China (English)

    战廷正; 杨彦; 唐莉莉; 卢作超; 石焕焕

    2010-01-01

    目的 观察广西人芽囊原虫分离株的苹果酸脱氢酶(MDH)和碱性磷酸酶(AIJP)同工酶谱特征.方法 从感染者粪便中分离人芽囊原虫.体外培养,收集虫体,制备电泳样品.采取不连续性聚丙烯酰胺凝胶垂直板状电泳分离同工酶的不同区带.以苹果酸钠和1.萘磷酸钠盐为特异性底物,氯化硝基四氮唑蓝(NBT)和固蓝RR盐为染色剂,分别对MDH和ALP染色,以相对迁移率标记酶带.结果 人芽囊原虫10个分离株在MDH酶谱中.共出现7条酶带,常见的酶带有Rm34、Rm47、Rm51、Rm55和Rm59,10个分离株完全一致的酶带有Rm34和Rm51:在ALP酶谱中共出现5条酶带,Rm22、Rm25、RM28、Rm35和Rm38.各分离株之间的MDH和ALP同工酶谱均存在差异.结论 MDH和ALP同工酶谱能反映人芽囊原虫各分离株之间的遗传差异.

  12. 人芽囊原虫感染致慢性腹泻18例临床分析%Analysis of 18 chronic diarrhea patients due to infection with Blastocystis hominis

    Institute of Scientific and Technical Information of China (English)

    徐凤全; 林玲; 李军; 时发茂

    2004-01-01

    目的分析人芽囊原虫感染致慢性腹泻病人临床资料,评价人芽囊原虫病的临床诊断及治疗效果. 方法诊断用直接涂片法检查病人粪便中的人芽囊原虫包囊;治疗用甲硝唑,成人250~400mg/次,3次/d,7d为一疗程.结果18例慢性腹泻病人病程为3~24个月,粪便中均查到人芽囊原虫包囊(包括阿米巴型、空泡型和颗粒型);用甲硝唑治疗的治愈率1个疗程为38.89%,2个疗程为100%. 结论粪便直接涂片镜检原虫包囊是确诊人芽囊原虫感染的有效方法,用甲硝唑治疗人芽囊原虫感染所致的慢性腹泻疗效显著.

  13. Observation on the effect of three drugs on Blastocystis hominis%三种药物对体外人芽囊原虫的作用效果观察

    Institute of Scientific and Technical Information of China (English)

    田春林; 万孝玲; 刘晓泉

    2007-01-01

    目的 了解甲硝唑、替硝唑和阿奇霉素对人芽囊原虫的作用效果. 方法 从门诊病人阳性粪便中分离出人芽囊原虫,接种到96孔无菌培养板中,实验组加入不同药物浓度的甲硝唑、替硝唑和阿奇霉素,对照组加入无菌生理盐水,应用细胞增殖实验方法检测三种药物对人芽囊原虫的抑制和杀灭作用. 结果 药物作用72h后,甲硝唑、替硝唑和阿奇霉素药物浓度为5μg/ml时相对抑制率分别为5.45%、16.13%和11.23%,光镜下原虫形态发生了改变. 结论 甲硝唑、替硝唑和阿奇霉素对体外人芽囊原虫的生长具有抑制和杀灭作用,替硝唑的作用效果最好.

  14. Observation on the growth status of Blastocystis hominis in different mediums%人芽囊原虫在不同培养基中生长状况的观察

    Institute of Scientific and Technical Information of China (English)

    答嵘; 乔继英; 卢朝辉; 李小琪; 李亚青; 王伟

    2006-01-01

    目的筛选培养人芽囊原虫的最适培养基. 方法将同一株人芽囊原虫阳性粪便标本以2×105细胞/管接种至RPMI1640、199和LES培养基中,加入20%小牛血清及青、链霉素,pH值为7.5,放置厌氧罐中于37 ℃恒温培养,每24 h计数,每6 d转种1次.观察人芽囊原虫在3种培养基中的存活时间、虫体密度和虫体形态. 结果人芽囊原虫在RPMI1640培养基中存活时间最长、虫体密度最高,虫体以空泡型多见;在LES培养基中存活时间最短、虫体密度最低,但虫体形态清晰、规则;在199培养基中存活时间和虫体密度均介于前两者之间. 结论 RPMI1640培养基适宜人芽囊原虫的生长繁殖,为人芽囊原虫体外培养的首选培养基;LES培养基中虫体形态清晰、规则,可用于人芽囊原虫的形态学研究;199培养基也可用于人芽囊原虫的体外培养,但不作为首选.

  15. CLINICAL STUDY ON THE EFFECT OF ORAL METRONIDAZOLE IN TREATMENT OF BLASTOCYSTIS HOMINIS INFECTION%口服甲硝唑治疗人芽囊原虫感染的临床研究

    Institute of Scientific and Technical Information of China (English)

    金群馨; 姜海行; 唐国都; 唐连凤; 田春林; 卢作超

    2005-01-01

    目的观察甲硝唑治疗人芽囊原虫感染的疗效. 方法 115例患者因腹疼、腹泻及粘液血便行肠镜检查,肠镜下取病变粘膜做病理检查,并采集其新鲜粪便,行直接涂片法和三色染色法用光学显微镜检查,确诊人芽囊原虫阳性而其他寄生虫阴性者,予口服甲硝唑0.4 g/d,4次/d,连续服7 d.治疗3周后粪检仍阳性者,再重复用药7 d.30例患者治疗后复查电子肠镜,观察肠粘膜损伤的变化. 结果甲硝唑治疗人芽囊原虫感染的总有效率为94.65%(230/243),其中服药7 d的治愈率76.54%(186/243);服药14 d的治愈率18.11%(44/243);治疗无效5.35%(13/243).部分患者服药后出现恶心、呕吐、食欲不振等不良反应,占4.53%(11/243). 电子结肠镜检查,所检患者的肠道病变部位主要在左半结肠、直肠,表现为粘膜糜烂及溃疡;病理检查多为粘膜慢性炎症或溃疡病变.治疗后复查肠镜30例,肠粘膜损伤均获治愈. 结论人芽囊原虫感染可引起肠粘膜糜烂及溃疡,口服甲硝唑治疗该病引起的肠粘膜损伤有较好的疗效,但少数患者出现药物不良反应或因产生抗药性而治疗无效.

  16. 3种培养基体外培养人芽囊原虫的效果观察%EFFECTIVE OBSERVATION ON CULTIVATION OF BLASTOCYSTIS HOMINIS IN THREE MEDIA IN VITRO

    Institute of Scientific and Technical Information of China (English)

    苏水莲; 严宜明; 陈桂凤; 廖华; 张瑞其; 胡雅琼

    2004-01-01

    目的观察人芽囊原虫在3种培养基中的生长繁殖情况,以探讨最佳的培养方法. 方法从大学生粪便中分离人芽囊原虫,转种至3种不同培养基,定时观察人芽囊原虫的生长繁殖情况,根据原虫密度评价培养效果. 结果培养96 h后,Locke's鸡蛋血清(LES)双相培养基中的人芽囊原虫密度最高,改良Jones单相液体培养基次之, Locke's琼脂血清(LAS)双相培养基原虫密度最低,差异有显著性(P<0.05);观察3种培养基中的原虫密度高峰持续时间分别为360 h、96 h和72 h,最长存活时间分别为552 h、336 h和168 h. 结论 Locke's鸡蛋血清双相培养基较适用于人芽囊原虫体外增殖培养.

  17. Investigation of infectious status of Blastocystis hominis in 1 354 outpatients%1354例门诊腹泻病人人芽囊原虫感染情况调查

    Institute of Scientific and Technical Information of China (English)

    金群馨; 俞开敏; 唐连凤; 田春林; 卢作超

    2005-01-01

    目的了解消化门诊腹泻就诊者中人芽囊原虫感染情况及其特征.方法询问腹泻患者的病史、卫生习惯及生活环境等状况.收集患者当日新鲜大便,用生理盐水直接涂片和碘液染色镜检初筛后,将疑似阳性的标本制片及三色染色法染色,在油镜下观察形态并测量大小. 结果共查粪便1 354份,人芽囊原虫检出率为18.54%,男女腹泻者检出率分别为18.12%和19.18%,差异无显著性(P>0.05).在人芽囊原虫混合其他寄生虫感染中,以肝吸虫占绝大多数(73.08%).粪检所见的原虫以空泡型为主,感染高峰在夏秋季节,高发年龄是21~50岁. 结论广西地区人芽囊原虫是引起腹泻的常见肠道寄生虫,其致病作用不容忽视.感染后可引发腹痛、腹泻等消化道症状.人芽囊原虫混合肝吸虫感染为广西地区人芽囊原虫感染的一个显著特点.

  18. Antiprotozoal effects of Oregano oil and Brucea javanica on Blastocystis hominis in vitro%中药牛至油、鸦胆子对人芽囊原虫的体外杀灭作用

    Institute of Scientific and Technical Information of China (English)

    乔继英; 李利军; 张荣; 张旭; 蔡娟; 杨华

    2008-01-01

    目的 通过观察牛至油、鸦胆子对人芽囊原虫的体外杀灭作用,筛选治疗人芽囊原虫感染有效药物及浓度.方法 从腹泻患者新鲜粪便中分离人芽囊原虫,分别用含牛至油和鸦胆子1640培养基培养,药物设200、400、800、1 600 、3 200和6 400 μg/ml组.于加药24 h和 72 h作虫体计数,评价药物效果,试验设甲硝唑药物对照组和不加药空白对照组.结果 牛至油1 600、3 200 μg/ml作用72 h、6 400 μg/ml作用24 h,虫体全部死亡,最适杀虫体浓度为800 μg/ml;鸦胆子6 400 μg/ml 24 h虫体全部死亡,最适杀虫浓度为1 600~3 200 μg/ml;甲硝唑40 μg/ml 72 h、≥80 μg/ml 24 h虫体全部死亡.结论 牛至油和鸦胆子具有体外杀灭人芽囊原虫作用.且牛至油的杀虫效果强于鸦胆子.

  19. Research progress on morphology, taxonomy and genetic diversity of Blastocystis hominis%人芽囊原虫形态分类及遗传学多样性的研究进展

    Institute of Scientific and Technical Information of China (English)

    陈锡慰; 吴观陵

    2006-01-01

    通过光镜和电镜已广泛研究了人芽囊原虫的形态学,但它的生物学的许多其他方面仍处于未知领域.现在利用大量的各种分子学手段研究芽囊原虫,已使人们开始理解它的生物学.该综述着眼于人芽囊原虫最近研究的一些热点,包括分类学关系和遗传学的多样性以及物种形成等方面.

  20. INFECTION STATUS OF BLASTOCYSTIS HOMINIS IN DIARRHEAL PATIENTS AND ITS REPRODUCTION%人芽囊原虫在腹泻患者中的感染状况及繁殖方式

    Institute of Scientific and Technical Information of China (English)

    答嵘; 乔继英; 杨珺华; 李琛

    2004-01-01

    目的观察人芽囊原虫在腹泻患者中的感染状况及繁殖方式,为进一步研究其生理生化特性及致病机理奠定基础. 方法调查腹泻患者临床表现;用生理盐水涂片、碘液染色和铁苏木素染色法在光镜下观察其在粪便标本和培养物及动物模型肠内容物中形态、结构及繁殖方式. 结果健康人群和腹泻人群粪便标本中人芽囊原虫检出率分别为0.94 %(2/213)和24.73 %(46/186);人芽囊原虫与其它肠道寄生原虫有合并感染;有二分裂、内二芽生殖和裂体增殖3种繁殖方式. 结论人芽囊原虫在腹泻患者中有较高的感染率;易与结肠内阿米巴和其他非致病阿米巴合并感染;以3种无性生殖方式繁殖.

  1. Treatment of blastocystis hominis protozoosis with Baitouweng Decoction: A report of two cases%白头翁汤加减治疗人芽囊原虫病2例

    Institute of Scientific and Technical Information of China (English)

    韩捷; 马贵同

    2003-01-01

    @@ 人芽囊原虫病是由人芽囊原虫所致的一种以排果酱样便的腹泻为主要症状的疾病,其症状与痢疾相似,临床很少遇见[1].笔者于2002年6~8月在上海龙华医院实习时见到两例入芽囊原虫病,运用经方白头翁汤加减口服并灌肠治疗后取得良好疗效,现报道如下.

  2. Epidemiological characteristics of Blastocystis hominis in urban region, southwestern China%我国西南城市地区人芽囊原虫的流行病学特征

    Institute of Scientific and Technical Information of China (English)

    张顺先; 田利光; 卢艳; 李兰花; 陈家旭; 周晓农

    2016-01-01

    目的 了解我国城市地区入芽囊原虫的流行病学特征和基因型分布,为人芽囊原虫的防治提供科学参考依据.方法 在云南省昆明市4家哨点医院收集腹泻病例粪便样本1121份,非腹泻人群粪便样本319份,使用结构化问卷收集研究对象基本信息,提取粪便基因组并采用PCR方法进行检查,对可疑阳性PCR产物进行测序,通过序列比对进行确诊,并通过进化树构建进行基因分型.结果 人芽囊原虫在全年龄组腹泻病例中检出率为4.2%;在女性腹泻人群中的检出率高于男性腹泻人群中的检出率(5.5%,2.9%;P=0.027,OR=1.98,95%CI=1.07-3.67);在不同年龄组腹泻人群中,人芽囊原虫的检出率没有差异(x2 =3.933,P=0.950);但具有明显季节分布特征(x2=11.8,P<0.05);人芽囊原虫的检出率在城区、农村以及城乡结合部的腹泻人群中没有差异(x2 =2.427,P=0.297).在腹泻人群和非腹泻人群中,人芽囊原虫Ⅰ型基因型都占绝对优势.结论 人芽囊原虫依旧是西南城市地区感染人群的常见肠道寄生虫之一,感染主要以Ⅰ型基因型为主,其致病性和基因型分布需持续研究和探索.

  3. A high-affinity human monoclonal IgM antibody reacting with multiple strains of Mycoplasma hominis

    DEFF Research Database (Denmark)

    Moller, SA; Birkelund, Svend; Borrebaeck, CA

    1990-01-01

    Human monoclonal antibodies were produced against Mycoplasma hominis by in vitro immunization of peripheral blood lymphocytes from a healthy seropositive donor using low amounts of antigen (5 ng/ml). The immune B lymphocytes were subsequently immortalized by Epstein-Barr virus transformation...

  4. Draft Genome Sequence of a Cardiobacterium hominis Strain Isolated from Blood Cultures of a Patient with Infective Endocarditis

    Science.gov (United States)

    Tagini, Florian; Pillonel, Trestan; Asner, Sandra; Prod’hom, Guy

    2016-01-01

    Cardiobacterium hominis is a well-known commensal bacterium of the oral cavity and an agent of infective endocarditis in humans. Here, we provide a draft genome sequence of a pathogenic strain isolated from blood cultures of a patient with infectious endocarditis. PMID:27660783

  5. In Vitro Development of Resistance to Six and Four Fluoroquinolones in Mycoplasma pneumoniae and Mycoplasma hominis, Respectively

    OpenAIRE

    Gruson, D; S. Pereyre; Renaudin, H.; Charron, A.; Bébéar, C.; Bébéar, C. M.

    2005-01-01

    Selection of resistant mutants in sequential subcultures with increasing concentrations of six and four different fluoroquinolones was studied for one reference strain each of Mycoplasma pneumoniae and Mycoplasma hominis, respectively. All fluoroquinolones tested selected for resistance, with alterations affecting the quinolone resistance-determining regions of the four target topoisomerase genes.

  6. Draft Genome Sequence of a Cardiobacterium hominis Strain Isolated from Blood Cultures of a Patient with Infective Endocarditis.

    Science.gov (United States)

    Tagini, Florian; Pillonel, Trestan; Asner, Sandra; Prod'hom, Guy; Greub, Gilbert

    2016-01-01

    Cardiobacterium hominis is a well-known commensal bacterium of the oral cavity and an agent of infective endocarditis in humans. Here, we provide a draft genome sequence of a pathogenic strain isolated from blood cultures of a patient with infectious endocarditis. PMID:27660783

  7. Morphology of human Fallopian tupes after infection with Mycoplasma genitalium and Mycoplasma hominis - in vitro organ culture study

    DEFF Research Database (Denmark)

    Baczynska, Agata; Funch, P.; Fedder, J.;

    2006-01-01

    -terminal part of the adhesion protein MgPa before infection of HFT organ culture. CONCLUSION We have shown that the presence of M. genitalium, but not M. hominis, in the HFT organ culture affected the epithelium and resulted in cilia damage. The effect of infection with M. genitalium on the HFT was, however...

  8. Morphology of human Fallopian tubes after infection with Mycoplasma genitalium and Mycoplasma hominis - in vitro organ culture study

    DEFF Research Database (Denmark)

    Baczynska, A.; Funch, Peter; Fedder, J.;

    2007-01-01

    against the C-terminal part of the adhesion protein MgPa before infection of HFT organ culture. CONCLUSION: We have shown that the presence of M. genitalium, but not M. hominis, in the HFT organ culture affected the epithelium and resulted in cilia damage. The effect of infection with M. genitalium...

  9. Morphology of human Fallopian tubes after infection with Mycoplasma genitalium and Mycoplasma hominis--in vitro organ culture study

    DEFF Research Database (Denmark)

    Baczynska, Agata; Funch, P; Fedder, J;

    2007-01-01

    against the C-terminal part of the adhesion protein MgPa before infection of HFT organ culture. CONCLUSION: We have shown that the presence of M. genitalium, but not M. hominis, in the HFT organ culture affected the epithelium and resulted in cilia damage. The effect of infection with M. genitalium...

  10. Inhibition of enteric parasites by emulsified oil of oregano in vivo.

    Science.gov (United States)

    Force, M; Sparks, W S; Ronzio, R A

    2000-05-01

    Oil of Mediterranean oregano Oreganum vulgare was orally administered to 14 adult patients whose stools tested positive for enteric parasites, Blastocystis hominis, Entamoeba hartmanni and Endolimax nana. After 6 weeks of supplementation with 600 mg emulsified oil of oregano daily, there was complete disappearance of Entamoeba hartmanni (four cases), Endolimax nana (one case), and Blastocystis hominis in eight cases. Also, Blastocystis hominis scores declined in three additional cases. Gastrointestinal symptoms improved in seven of the 11 patients who had tested positive for Blastocystis hominis. PMID:10815019

  11. Blastocystis Isolates from Patients with Irritable Bowel Syndrome and from Asymptomatic Carriers Exhibit Similar Parasitological Loads, but Significantly Different Generation Times and Genetic Variability across Multiple Subtypes.

    Directory of Open Access Journals (Sweden)

    Gie-Bele Vargas-Sanchez

    Full Text Available Blastocystis spp is a common intestinal parasite of humans and animals that has been associated to the etiology of irritable bowel syndrome (IBS; however, some studies have not found this association. Furthermore, many biological features of Blastocystis are little known. The objective of present study was to assess the generation times of Blastocystis cultures, from IBS patients and from asymptomatic carriers. A total of 100 isolates were obtained from 50 IBS patients and from 50 asymptomatic carriers. Up to 50 mg of feces from each participant were cultured in Barret's and in Pavlova's media during 48 h. Initial and final parasitological load were measured by microscopy and by quantitative PCR. Amplicons were purified, sequenced and submitted to GenBank; sequences were analysed for genetic diversity and a Bayesian inference allowed identifying genetic subtypes (ST. Generation times for Blastocystis isolates in both media, based on microscopic measures and molecular assays, were calculated. The clinical symptoms of IBS patients and distribution of Blastocystis ST 1, 2 and 3 in both groups was comparable to previous reports. Interestingly, the group of cases showed scarce mean nucleotide diversity (π as compared to the control group (0.011±0.016 and 0.118±0.177, respectively, whilst high gene flow and small genetic differentiation indexes between different ST were found. Besides, Tajima's D test showed negative values for ST1-ST3. No statistical differences regarding parasitological load between cases and controls in both media, as searched by microscopy and by qPCR, were detected except that parasites grew faster in Barret's than in Pavlova's medium. Interestingly, slow growth of isolates recovered from cases in comparison to those of controls was observed (p<0.05. We propose that generation times of Blastocystis might be easily affected by intestinal environmental changes due to IBS probably because virulent strains with slow growth may be

  12. Prevalence and genetic diversity of the intestinal parasites Blastocystis sp. and Cryptosporidium spp. in household dogs in France and evaluation of zoonotic transmission risk.

    Science.gov (United States)

    Osman, Marwan; Bories, Jessica; El Safadi, Dima; Poirel, Marie-Thérèse; Gantois, Nausicaa; Benamrouz-Vanneste, Sadia; Delhaes, Laurence; Hugonnard, Marine; Certad, Gabriela; Zenner, Lionel; Viscogliosi, Eric

    2015-11-30

    Several parasites including the protozoa Blastocystis sp. and Cryptosporidium spp. may be causative agents of gastrointestinal symptoms in domestic dogs, and there may be a potential risk of transmission to owners. While France is one of the largest European countries in terms of its canine population, little data is available about the molecular epidemiology of these two parasites. The purpose of this study was to determine the prevalence of intestinal parasites in household dogs in France, and to evaluate the zoonotic risk of Blastocystis sp. and Cryptosporidium spp. by genotyping the corresponding isolates. To this end, 116 faecal samples were collected from household dogs regardless of breed, age or gender, living in the Lyons area, France. Various intestinal protozoa and helminths were identified by light microscopy. Screening for Blastocystis sp. and Cryptosporidium spp. were subsequently performed by PCR targeting the small subunit (SSU) rDNA coding region, followed by direct sequencing of the PCR products and analysis of the sequences obtained for genotyping. The overall prevalence of dogs infected with at least one gastrointestinal parasite was 42.2% (49/116). After light microscopy examination of faecal samples, the most common parasites found were the protozoa Giardia sp. (25.0%) and Cystoisospora sp. (19.8%). Using molecular methods, four dogs (3.4%) were shown to be infected by Blastocystis sp. and carried either subtype (ST) 2, commonly identified in various animal groups, or ST10, frequently found in bovids. Three dogs (2.6%) were positive for C. canis, infecting humans episodically. The low prevalence of both parasites, combined with the identification of C. canis and Blastocystis sp. ST2 and ST10 in the canine population, strongly suggests that dogs play a negligible role as zoonotic reservoirs for both parasites and do not seem to be natural hosts of Blastocystis sp. PMID:26395822

  13. Prevalence and genetic diversity of the intestinal parasites Blastocystis sp. and Cryptosporidium spp. in household dogs in France and evaluation of zoonotic transmission risk.

    Science.gov (United States)

    Osman, Marwan; Bories, Jessica; El Safadi, Dima; Poirel, Marie-Thérèse; Gantois, Nausicaa; Benamrouz-Vanneste, Sadia; Delhaes, Laurence; Hugonnard, Marine; Certad, Gabriela; Zenner, Lionel; Viscogliosi, Eric

    2015-11-30

    Several parasites including the protozoa Blastocystis sp. and Cryptosporidium spp. may be causative agents of gastrointestinal symptoms in domestic dogs, and there may be a potential risk of transmission to owners. While France is one of the largest European countries in terms of its canine population, little data is available about the molecular epidemiology of these two parasites. The purpose of this study was to determine the prevalence of intestinal parasites in household dogs in France, and to evaluate the zoonotic risk of Blastocystis sp. and Cryptosporidium spp. by genotyping the corresponding isolates. To this end, 116 faecal samples were collected from household dogs regardless of breed, age or gender, living in the Lyons area, France. Various intestinal protozoa and helminths were identified by light microscopy. Screening for Blastocystis sp. and Cryptosporidium spp. were subsequently performed by PCR targeting the small subunit (SSU) rDNA coding region, followed by direct sequencing of the PCR products and analysis of the sequences obtained for genotyping. The overall prevalence of dogs infected with at least one gastrointestinal parasite was 42.2% (49/116). After light microscopy examination of faecal samples, the most common parasites found were the protozoa Giardia sp. (25.0%) and Cystoisospora sp. (19.8%). Using molecular methods, four dogs (3.4%) were shown to be infected by Blastocystis sp. and carried either subtype (ST) 2, commonly identified in various animal groups, or ST10, frequently found in bovids. Three dogs (2.6%) were positive for C. canis, infecting humans episodically. The low prevalence of both parasites, combined with the identification of C. canis and Blastocystis sp. ST2 and ST10 in the canine population, strongly suggests that dogs play a negligible role as zoonotic reservoirs for both parasites and do not seem to be natural hosts of Blastocystis sp.

  14. A Pilot Study on Single-dose Toxicity Testing of Hominis placenta Pharmacopuncture in Sprague-Dawley Rats

    Directory of Open Access Journals (Sweden)

    Yoo-Hwan Lee

    2015-06-01

    Full Text Available Objectives: This study was performed to analyze the toxicity and to find the lethal dose of the test substance Hominis placenta pharmacopuncture when used as a single-dose in 6 week old, male and female Sprague-Dawley (SD rats. Methods: All experiments were conducted at Biotoxtech (Chungwon, Korea, an institution authorized to perform non clinical studies, under the regulations of Good Laboratory Practice (GLP. SD rats were chosen for the pilot study. Doses of Hominis placenta pharmacopuncture extracts, 0.125, 0.25 and 0.5 mL, were administered to the experimental group, and 0.5 mL doses of normal saline solution were administered to the control group. This study was conducted under the approval of the Institutional Animal Ethics Committee. Results: No deaths or abnormalities occurred in any of the groups. Also, no significant changes in body weights were observed among the groups, and no significant differences in hematology/biochemistry, necropsy, and histopathology results were noted. Hematologically, some changes in the male rats in two experimental groups were observed, but those changes had no clinical or toxicological meaning because they were not dose dependent. Histopathological tests on the injected parts showed cell infiltration in the male rats in one of the experimental groups; however, that result was due to spontaneous generation and had no toxicological meaning. Therefore, this study showed that Hominis placenta pharmacopuncture had no effect on the injected parts in terms of clinical signs, body weight, hematology, clinical chemistry, and necropsy. Conclusion: As a result of single-dose tests of the test substance Hominis placenta pharmacopuncture in 4 groups of rats, the lethal dose for both males and females exceeded 0.5 mL/animal. Therefore, the above findings suggest that treatment with Hominis placenta pharmacopuncture is relatively safe. Further studies on this subject are needed.

  15. Ureaplasma urealyticum, Ureaplasma parvum, Mycoplasma hominis and Mycoplasma genitalium infections and semen quality of infertile men

    Directory of Open Access Journals (Sweden)

    Rebai Tarek

    2007-11-01

    Full Text Available Abstract Background Genital ureaplasmas (Ureaplasma urealyticum and Ureaplasma parvum and mycoplasmas (Mycoplasma genitalium and Mycoplasma hominis are potentially pathogenic species playing an etiologic role in both genital infections and male infertility. Reports are, however, controversial regarding the effects of these microorganisms infections in the sperm seminological variables. This study aimed at determining the frequency of genital ureplasmas and mycoplasmas in semen specimens collected from infertile men, and at comparing the seminological variables of semen from infected and non-infected men with these microorganisms. Methods A total of 120 semen samples collected from infertile men were investigated. Semen specimens were examined by in-house PCR-microtiter plate hybridization assay for the presence of genital ureaplasmas and mycoplasmas DNA. Semen analysis was assessed according to the guidelines of the World Health Organization. Standard parametric techniques (t-tests and nonparametric techniques (Wilcoxon tests were used for statistical analysis. Results The frequency of genital ureaplasmas and mycoplasmas detected in semen samples of infertile men was respectively 19.2% (23/120 and 15.8% (19/120. The frequency of Ureaplasma urealyticum (15% was higher than that of Mycoplasma hominis (10.8%, Ureaplasma parvum (4.2% and Mycoplasma genitalium (5%. Mixed species of mycoplasmas and ureaplasmas were detected in 6.7% of semen samples. Comparison of the parameters of the standard semen analysis between the male partners of the infertile couples with and without genital ureaplasmas and mycoplasmas infection showed that the presence of Mycoplasma hominis DNA in semen samples is associated with low sperm concentration (p = 0.007 and abnormal sperm morphology (p = 0.03 and a negative correlation between sperm concentration and the detection of Mycoplasma genitalium in semen samples of infertile men (p = 0.05. The mean values of seminal

  16. Blastocystis is associated with decrease of fecal microbiota protective bacteria: Comparative analysis between patients with irritable bowel syndrome and control subjects

    OpenAIRE

    Céline Nourrisson; Julien Scanzi; Bruno Pereira; Christina NkoudMongo; Ivan Wawrzyniak; Amandine Cian; Eric Viscogliosi; Valérie Livrelli; Frédéric Delbac; Michel Dapoigny; Philippe Poirier

    2014-01-01

    Blastocystis is a protistan parasite living in the digestive tract of many animals, including humans. This highly prevalent intestinal parasite is suspected to be linked to Irritable Bowel Syndrome (IBS), a chronic functional bowel disorder. Here, we first compared the prevalence of Blastocystis among 56 IBS patients (40 IBS with constipation (IBS-C), 9 IBS with diarrhea (IBS-D), 4 mixed IBS (IBS-M) and 3 unsubtyped IBS (IBS-U) according to the Rome III criteria) and 56 control (i.e. without ...

  17. Prevalence and Risk Factors for Intestinal Protozoan Infections with Cryptosporidium, Giardia, Blastocystis and Dientamoeba among Schoolchildren in Tripoli, Lebanon.

    Directory of Open Access Journals (Sweden)

    Marwan Osman

    2016-03-01

    Full Text Available Intestinal protozoan infections are confirmed as major causes of diarrhea, particularly in children, and represent a significant, but often neglected, threat to public health. No recent data were available in Lebanon concerning the molecular epidemiology of protozoan infections in children, a vulnerable population at high risk of infection.In order to improve our understanding of the epidemiology of intestinal pathogenic protozoa, a cross-sectional study was conducted in a general pediatric population including both symptomatic and asymptomatic subjects. After obtaining informed consent from the parents or legal guardians, stool samples were collected in January 2013 from 249 children in 2 schools in Tripoli, Lebanon. Information obtained from a standard questionnaire included demographic characteristics, current symptoms, socioeconomic status, source of drinking water, and personal hygiene habits. After fecal examination by both microscopy and molecular tools, the overall prevalence of parasitic infections was recorded as 85%. Blastocystis spp. presented the highest infection rate (63%, followed by Dientamoeba fragilis (60.6%, Giardia duodenalis (28.5% and Cryptosporidium spp. (10.4%. PCR was also performed to identify species and genotypes of Cryptosporidium, subtypes of Blastocystis, and assemblages of Giardia. Statistical analysis using a logistic regression model showed that contact with family members presenting gastrointestinal disorders was the primary risk factor for transmission of these protozoa.This is the first study performed in Lebanon reporting the prevalence and the clinical and molecular epidemiological data associated with intestinal protozoan infections among schoolchildren in Tripoli. A high prevalence of protozoan parasites was found, with Blastocystis spp. being the most predominant protozoans. Although only 50% of children reported digestive symptoms, asymptomatic infection was observed, and these children may act as

  18. Clinical Review of the Effects of Hominis Placental Pharmacopuncture in the Treatment of Facial Spasm Patients

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    Jo Na-Young

    2013-09-01

    Full Text Available Objectives: The main purpose of this research is to investigate the effect of treatment with Hominis Placental pharmacopuncture (HPP for 32 patients with hemifacial spasm. Methods: We treated facial spasm patients with acupuncture and HPP at Sabaek (ST2, Seung-eup (ST1, Gwallyeo (SI18, Chanjuk (BL2, Sajukgong (TE23, Hagwan (ST7, Hyeopgeo (ST6, Jichang (ST4, Wan-gol (SI4 and Yepung (TE17, and we investigated the effect by using Scott’s scale. The data were analyzed by using the SPSS/10.0 for windows program with descriptive statistics, the paired t-test, and the Shapiro-Wilk normality test. Results: After treatment, the grade of the spasm’s intensity based on Scott’s description were decreased significantly. About 72% of the patients felt that the combination treatment had produced excellent results. Conclusion: These data suggested that HPP can be useful for treating facial spasm patients.

  19. Heterogeneity and compartmentalization of Pneumocystis carinii f. sp. hominis genotypes in autopsy lungs

    DEFF Research Database (Denmark)

    Helweg-Larsen, J; Lundgren, Bettina; Lundgren, Jens Dilling

    2001-01-01

    The extent and importance of genotype heterogeneity of Pneumocystis carinii f. sp. hominis within lungs have not previously been investigated. Two hundred forty PCR clones obtained from respiratory specimens and lung segments from three patients with fatal P. carinii pneumonia were investigated....... Not all genotypes present in the lungs at autopsy were detected in the diagnostic respiratory samples. Compartmentalization of specific ITS and mtLSU rRNA sequence types was observed in different lung segments. In conclusion, the interpretation of genotype data and in particular ITS sequence types...... in the assessment of epidemiological questions should be cautious since genotyping done on respiratory samples cannot a priori be assumed to represent all genotypes present within the lung....

  20. The Mycoplasma hominis P120 membrane protein contains a 216 amino acid hypervariable domain that is recognized by the human humoral immune response

    DEFF Research Database (Denmark)

    Nyvold, Charlotte Guldborg; Birkelund, Svend; Christiansen, Gunna

    1997-01-01

    In the antigenically heterogeneous species Mycoplasma hominis a monoclonal antibody, mAb 26.7D, was previously found to recognize a 120 kDa polypeptide from M. hominis 7488. This antibody did not react with the type strain PG21. The homologous gene from M. hominis PG21 was cloned and sequenced...... with the 7488 hypervariable fusion protein, but only four reacted with PG21 hypervariable fusion protein. No reactivity was seen with a fusion protein containing part of the constant region of P120. Gene fragments amplified from 18 M. hominis isolates by PCR confirmed the heterogeneity of the hypervariable...... domain. Based on restriction endonuclease cleavage patterns of the hypervariable domain the 18 isolates could be divided into four cases. Reactivity with both mAb 26.7D and pAb 121 confirmed these classes. The hypervariable, but not the constant, part of P120 was recognized by the human humoral immune...

  1. Phylogeny of some mycoplasmas from ruminants based on 16S rRNA sequences and definition of a new cluster within the hominis group.

    Science.gov (United States)

    Pettersson, B; Uhlén, M; Johansson, K E

    1996-10-01

    Almost complete (> 96%) 16S rRNA sequences from nine ruminant mycoplasmas have been determined by solid-phase DNA sequencing. Polymorphisms were found in four of the 16S rRNA sequences, which indicated the existence of two different rRNA operons. Seven polymorphisms were found in Mycoplasma agalatiae, three were found in Mycoplasma bovis, one was found in Mycoplasma alkalescens, and one was found in Mycoplasma bovirhinis. The sequence data were used for construction of phylogenetic trees. All but one of the ruminant mycoplasmas sequenced in this work clustered in the hominis group. A close relationship was found between M. agalactiae and M. bovis, with a 99% nucleotide similarity between their 16S rRNA sequences. They were also found to be members of the Mycoplasma lipophilum cluster of the hominis group. Furthermore, the 16S rRNA comparisons showed that Mycoplasma alkalescens and Mycoplasma canadense are closely related (> 98.5%), and these species were found to cluster in the Mycoplasma hominis cluster of the hominis group. Interestingly, M. bovirhinis grouped in a new phylogenetic cluster of the hominis group. The new cluster, which was supported by bootstrap percentage values, signature nucleotide analysis, and higher-order structural elements, was named the Mycoplasma synoviae cluster. Mycoplasma bovoculi, Mycoplasma conjunctivae, and Mycoplasma ovipneumoniae clustered in the Mycoplasma neurolyticum cluster of the hominis group. Mycoplasma alvi clustered with Mycoplasma pirum in the M. pneumoniae cluster of the pneumoniae group.

  2. Concurrent infections with Cryptosporidium spp., Giardia duodenalis, Enterocytozoon bieneusi, and Blastocystis spp. in naturally infected dairy cattle from birth to two years of age

    Science.gov (United States)

    Fecal specimens were collected directly at weekly and then monthly intervals from each of 30 dairy calves from birth to 24 months to determine the prevalence and age distribution of Cryptosporidium spp., Giardia duodenalis assemblages, Enterocytozoon bieneusi genotypes, and Blastocystis spp subtypes...

  3. Comparison of direct fecal smear microscopy, culture, and polymerase chain reaction for the detection ofBlastocystis sp. in human stool samples

    Institute of Scientific and Technical Information of China (English)

    Herbert J Santos; Windell L Rivera

    2013-01-01

    Objective:To compare the sensitivity and specificity of direct fecal smear microscopy, culture, and polymerase chain reaction in the detection ofBlastocystis sp. in human stool. Methods:Human stool samples were collected from a community inSanIsidro,Rodriguez, Rizal,Philippines.These samples were subjected to direct fecal smear microscopy, culture and polymerase chain reaction to detect the presence of Blastocystissp.Results:Of the110 stool samples collected,28(25%) were detected positive for the presence ofBlastocystis sp. by two or more tests.Culture method detected the highest number ofBlastocystis-positive stool samples (n=36), followed byPCR ofDNA extracted from culture(n=26),PCR ofDNA extracted from stool (n=10), and direct fecal smear(n=9).Compared to culture, the sensitivity of the other detection methods were66.7% forPCR from culture and19.4% for bothPCR from stool and direct fecal smear.Specificity of the methods was high, withPCR from culture and direct fecal smear having 97.3%, whilePCR from stool at95.9%.Conclusions:In this study,in vitroculture is the best method for detectingBlastocystis sp. in human stool samples.

  4. A Clinical Study on 1 Case of Patient with Bilateral Simultaneous Bell's Palsy Treated by Hominis Placenta Herbal-Acupuncture

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    Kwon, Kang

    2003-06-01

    Full Text Available Objective : This study was carried out to investigate the progress of bilateral simultaneous facial palsy and the effect of Hominis Placenta herbal-acupunture and the other oriental medical therapies. Methods : We used two methods to research the progress of disease. 1. Diagnosis - Facial muscle test, Taste test, Hearing test, Photographies, Lab-finding 2. Treatment - Acupuncture, Herbal-acupuncture, Electroacupuncture, Herb-med Results : The onset of Rt. facial palsy was earlier than Lt. facial palsy 3days. The reaction on the treatment of Rt. facial palsy was more dull than Lt. facial palsy. In terms of treatment period, Rt. facial palsy was very longer than Lt. facial palsy. Conclusion : According to the above results, we discoveried that Hominis Placenta herbal-acupunture and the other oriental medical therapies had good influence on the bilateral simultaneous facial palsy. In the future, we should endeavor to know influence between Rt. and Lt. face in case of bilateral simultaneous Bell's palsy.

  5. Fatal nosocomial meningitis caused by Mycoplasma hominis in an adult patient: case report and review of the literature.

    Science.gov (United States)

    Reissier, Sophie; Masson, Romain; Guérin, François; Viquesnel, Gérald; Petitjean-Lecherbonnier, Joëlle; Pereyre, Sabine; Cattoir, Vincent; Isnard, Christophe

    2016-07-01

    Meningitis due to Mycoplasma hominis in adults is rarely described, with only three cases having been reported to date. A case of fatal meningitis in a 39-year-old patient after a neurosurgical procedure for a subarachnoid haemorrhage is reported herein. Identification and treatment were significantly delayed because of the rarity of the aetiology and difficulty identifying this organism with the routinely used conventional methods, such as Gram staining and agar growth on standard agar plates. Clinical procedures and the treatment of 'culture-negative' central nervous system infections is a real challenge for clinical microbiologists and clinicians, and M. hominis has to be considered as a potential, although very uncommon, pathogen. PMID:27208637

  6. The genome of the obligate intracellular parasite Trachipleistophora hominis : new insights into microsporidian genome dynamics and reductive evolution

    OpenAIRE

    Williams, T.A.; Nakjang, S.; No\\xebl, C. J; Swan, D C; Goldberg, A. V; Harris, S. R.; Weinmaier, T; Markert, S; Becher, D.; Bernhardt, J.; Dagan, T.; Hacker, C.; Lucocq, J M; Schweder, T.; Rattei, T.

    2012-01-01

    The dynamics of reductive genome evolution for eukaryotes living inside other eukaryotic cells are poorly understood compared to well-studied model systems involving obligate intracellular bacteria. Here we present 8.5 Mb of sequence from the genome of the microsporidian Trachipleistophora hominis, isolated from an HIV/AIDS patient, which is an outgroup to the smaller compacted-genome species that primarily inform ideas of evolutionary mode for these enormously successful obligate intracellul...

  7. Effect of Hominis placenta Pharmacopuncture on the Dysmenorrhea (A Pilot study, Single blind, Randomized, Controlled Clinical Trial

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    Su-Min Kim

    2008-09-01

    Full Text Available Objective : This study was designed to evaluate the effect of Hominis placenta Pharmacopuncture treatment on Dysmenorrhea of Women. Methods : 49 subjects who were suffering from dysmenorrhea volunteered to answer the MMP(Measure of Menstrual Pain and MSSL(Menstrual Symptom Severity List questionnaire. They were divided into two groups, a Hominis placenta Pharmacopuncture treatment group(Experiment al group, n=25 and a Normal Saline(N/S treatment group(Control group, n=24. The two groups were injected on the CV4, S36, Sp9 and Sp6 acupuncture point. They were treated totally five times depending on the individual menstruation cycles. The scores of MMP and MSSL were measured overall three times before and after the menstruation cycle. The collected data were analyzed as paired t-test, independent t-test using SPSS 12.0 WIN Program. Results : As a result of the evaluation by MMP and MSSL, a significant improvement on dysmenorrhea was made in the two groups(p<0.05, and both scores of Experiment group were decreased more than Control group. But there was no significant difference between the two groups. Conclusions : The Hominis placenta Pharmacopuncture treatment and the Normal Saline treatment were effective in decreasing the symptom of Dysmenorrhea.

  8. The associated microflora to the larvae of human bot fly Dermatobia hominis L. Jr. (Diptera: Cuterebridae and its furuncular lesions in cattle

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    E Sancho

    1996-06-01

    Full Text Available The microflora associated to furuncular lesions, larvae and pupae of Dermatobia hominis, as well as the relationships between parasite, host and microflora associated, as a comprehensive microsystem, has been studied. One hundred and two furuncular myiasis due to D. hominis larvae in several breeds of cattle were studied and the following bacterial species were significant: Staphylococcus aureus, S. epidermidis, S. warneri, Bacillus subtilis and Escherichia coli. Closely related, the microflora associated to 141 samples from first, second, third instar larva and both external surface and larval cavities has been studied. The representative associated microflora to the larvae were: S. aureus, B. subtilis, S. hycus and Moraxella phenylpiruvica, Moerella wisconsiensis, Proteus mirabilis and P. vulgaris, M. phenylpiruvica, M. wisconsiensis, P. mirabilis and P. rettgeri were the representative microflora associated to 64 pupae of D. hominis.

  9. Detection of T. vaginalis,M. hominis,M. genitalium, C. trachomatis, N. gonorrhoeae and U. urealyticum using Multiplex PCR

    Directory of Open Access Journals (Sweden)

    Tamara Brunelli

    2013-03-01

    Full Text Available Intoduction. The sexually transmitted diseases include a large group of infections affecting both the sexes. In this study we evaluated the prevalence of Trichomonas vaginalis, Mycoplasma hominis, Mycoplasma genitalium, Chlamydia trachomatis, Neisseria gonorrhoeae and Ureaplasma urealyticum in the Prato area during the period September 2010 – July 2011. Methods.We analysed different kind of samples (urine, endocervical swabs, urethral swabs, seminal fluids from hospitalized patients or referred to the Prato clinic subjects.The DNA was obtained using EZ1-DNA extraction kit and EZ1 instrument.The DNA was then amplified using the Seeplex STD6 kit (Seegene, Korea, identifying multiple pathogens simultaneously (T. vaginalis, M. hominis, M. genitalium, C. trachomatis, N. gonorrhoeae e U. urealyticum. The revelation was performed by electrophoresis on microchip (instrument Multina, Shimadzu, Japan. Results. 1136 samples from Italian and foreign patients were examined: 876 were endocervical swabs (77%, 103 urethral swabs (9%, 103 seminal fluids (9%, and 54 urines (5%. The number of females was higher than males [894 (78.7% vs 242 (21.3%]; the mean age of females was 37.0±11.6 years, whereas that of males was 41.5 ±12.63 years.The prevalence of urogenital pathogens was: 15 positive samples for T. vaginalis (1.3%, 56 for M. hominis (4.9%, 13 for M. genitalium (1.1%, 28 for C. trachomatis (2.5%, 8 for N. gonorrhoeae (0.7% and 87 for U. urealyticum (7.7%.Among all positive, 25 subjects were positive for more than one pathogen and in particular: one was positive for the presence of 4 pathogens, five presented 3 pathogens simultaneously and the remaining nineteen for 2 pathogens. Conclusions. This study provides data on the prevalence of sexually transmitted diseases in the hospital of Prato.

  10. Cryptosporidium hominis Is a Newly Recognized Pathogen in the Arctic Region of Nunavik, Canada: Molecular Characterization of an Outbreak.

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    Karine Thivierge

    2016-04-01

    Full Text Available Cryptosporidium is a leading cause of childhood diarrhea in low-resource settings, and has been repeatedly associated with impaired physical and cognitive development. In May 2013, an outbreak of diarrhea caused by Cryptosporidium hominis was identified in the Arctic region of Nunavik, Quebec. Human cryptosporidiosis transmission was previously unknown in this region, and very few previous studies have reported it elsewhere in the Arctic. We report clinical, molecular, and epidemiologic details of a multi-village Cryptosporidium outbreak in the Canadian Arctic.We investigated the occurrence of cryptosporidiosis using a descriptive study of cases with onset between April 2013 and April 2014. Cases were defined as Nunavik inhabitants of any age presenting with diarrhea of any duration, in whom Cryptosporidium oocysts were detected by stool microscopy in a specialised reference laboratory. Cryptosporidium was identified in stool from 51 of 283 individuals. The overall annual incidence rate (IR was 420 / 100,000 inhabitants. The IR was highest among children aged less than 5 years (1290 /100,000 persons. Genetic subtyping for stool specimens from 14/51 cases was determined by DNA sequence analysis of the 60 kDa glycoprotein (gp60 gene. Sequences aligned with C. hominis subtype Id in all cases. No common food or water source of infection was identified.In this first observed outbreak of human cryptosporidiosis in this Arctic region, the high IR seen is cause for concern about the possible long-term effects on growth and development of children in Inuit communities, who face myriad other challenges such as overcrowding and food-insecurity. The temporal and geographic distribution of cases, as well as the identification of C. hominis subtype Id, suggest anthroponotic rather than zoonotic transmission. Barriers to timely diagnosis delayed the recognition of human cryptosporidiosis in this remote setting.

  11. Therapeutic effects of Hominis placenta herb-acupuncture in adjuvant-induced arthritis rat

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    MiJung Yeom

    2002-02-01

    Full Text Available Rheumatoid arthritis (RA is a chronic inflammatory autoimmune disease, characterized by leukocyte infiltration, a chronic inflammation of the joint, a pannus formation and the extensive destruction of the articular cartilage and bone. Several proinflammatory cytokines such as tumor necrosis factor-α (TNF-α, interleukin-1β (IL-1β and interleukin 6 (IL-6 have been implicated in the pathological mechanisms of synovial tissue proliferation, joint destruction and programmed cell death in rheumatoid joint. In the Korean traditional medicine, Hominis placenta (HP as an herbal solution of herb-acupuncture has been widely used to treat the inflammatory diseases including RA. In order to study the medicinal effect of HP herb-acupuncture on rheumatoid joint, an adjuvant-induced arthritis (AIA was generated by the injection of 1.5 mg of Mycobacterium tuberculosis, emulsified in squalene, to the base of the tail of Spraque-Dawley (SD rats. After onset stage of polyarthritis, HP was daily injected to the Zusanli (ST36 acupuncture points in both of rat lags and the expression patterns of cytokines such as TNF-α, IL-1β, and IL-6 at the knee joint were analyzed using immunostaining and RT-PCR. The HP herb-acupuncture was found to be effective to alleviate the arthritic symptoms in adjuvant-induced arthritic rats as regards the joint appearance and the expression profiles of inflammatory cytokines. In conclusion, therapeutic effects of HP herb-acupuncture on the rat with AIA might be related to anti-inflammatory activities of the hurb-acupuncture.

  12. Growth-promoting activity of Hominis Placenta extract on regenerating sciatic nerve

    Institute of Scientific and Technical Information of China (English)

    Tae-beom SEO; Dong-hee KIM; Seung-kiel PARK; Deok-chun YANG; Uk NAMGUNG; In-sun HAN; Jin-hwan YOON; In-chan SEOL; Yun-sik KIM; Hyun-kyung JO; Joung-jo AN; Kwon-eui HONG; Young-bae SEO

    2006-01-01

    Aim: Extract of Hominis Placenta (HP) has been used in oriental medicine as an agent for improving physiological function. The present study was conducted to investigate whether HP treatment in an experimental sciatic nerve injury animal model produces growth-promoting effects on regenerating peripheral nerve fibers after injury. Methods: After HP was injected into a sciatic nerve injury site, changes in protein levels were analyzed in the regenerating nerve area by Western blotting and immunofluorescence staining analyses. For quantitative assessment of axonal regeneration, a retrograde tracing technique was used to identify the neuronal cell bodies corresponding to regenerating axons, and the extent of neurite outgrowth in cultured dorsal root ganglia (DRG) sensory neurons prepared from animals that had experienced a sciatic nerve crush injury 7 d before neuron collection was analyzed. Results: Induction levels of axonal growth-associated protein (GAP-43) in the injured sciatic nerves were elevated by HP treatment. HP treatment also upregulated cell division cycle 2 (Cdc2) protein levels in the distal stump of the injured sciatic nerve. Induced Cdc2 protein was detected in Schwann cells, suggesting that Cdc2 kinase activity may be involved in the growth-promoting activity of regenerating axons via Schwann cell proliferation. Cell body measurement by retrograde tracing indicated that HP treatment produced significant increases in regenerating motor axons. Finally, HP treatment of cultured DRG sensory neurons significantly increased neurite arborization and elongation.Conclusion: HP promotes the regeneration of injured sciatic axons by upregulating the synthesis of regeneration-related protein factors such as GAP-43 and Cdc2.

  13. Prevalence and antibiotic susceptibility of Mycoplasma hominis and Ureaplasma urealyticum in genital samples collected over 6 years at a Serbian university hospital

    Directory of Open Access Journals (Sweden)

    Dusan Skiljevic

    2016-01-01

    Full Text Available Background: Mycoplasma hominis and Ureaplasma urealyticum are implicated in a wide array of infectious diseases in adults and children. Since some species have innate or acquired resistance to certain types of antibiotics, antibiotic susceptibility testing of mycoplasma isolated from the urogenital tract assumes increasing importance. Aims: To evaluate the prevalence and antibiotic susceptibility of M. hominis and U. urealyticum in genital samples collected between 2007 and 2012. Methods: Three hundred and seventy three patients presenting with symptoms of sexually transmitted diseases, infertility or risky sexual behaviour, who had not taken antibiotics in the previous 6 weeks and had ≥10 WBC per high power field on genital smears were studied. Urethral samples were taken in men and endocervical samples in women. The mycoplasma IST-2 kit was used for organism identification and for testing susceptibility to doxycycline, josamycin, ofloxacin, erythromycin, tetracycline, ciprofloxacin, azithromycin, clarithromycin and pristinamycin. Results: U. urealyticum was isolated from 42 patients and M. hominis from 11 patients. From 9.8% of isolates, both organisms were grown. All M. hominis isolates were resistant to tetracycline, clarithromycin and erythromycin while U. urealyticum was highly resistant to clarithromycin (94.6%, tetracycline (86.5%, ciprofloxacin (83.8% and erythromycin (83.8%. M. hominis was sensitive to doxycycline (83.3% and ofloxacin (66.7% while most U. urealyticum strains were sensitive to doxycycline (94.6%. Limitations: Inability of the commercial kit used in the study to detect other potentially pathogenic urogenital mycoplasmas (Ureaplasma parvum, Mycoplasma genitalium. Conclusion: There is significant resistance of U. urealyticum and M. hominis to tetracycline and macrolides. The most active tetracycline for genital mycoplasmas was found to be doxycycline, which continues to be the drug of first choice.

  14. PCR-Múltiple para el diagnóstico de Mycoplasma genitalium, Mycoplasma hominis, Ureaplasma parvum y Ureaplasma urealyticum

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    Nadia Rodríguez-Preval

    2007-04-01

    Full Text Available Mycoplasma genitalium, Mycoplasma hominis, Ureaplasma parvum y Ureaplasma urealyticum son especies relacionadas con enfermedades del tracto genitourinario, y particularmente con la uretritis no gonocócica (UNG en el hombre. Los cultivos de estos microorganismos resultan complicados, por lo que las técnicas moleculares, principalmente la reacción en cadena de la polimerasa (PCR, se han convertido en el principal método de detección de estos organismos. Objetivo: Implementar un método molecular basado en tecnología de genes para el diagnóstico de estas cuatro especies de micoplasmas genitales, aplicándolo en muestras clínicas de pacientes con UNG. Material y métodos: Se crearon las condiciones para un PCR-Múltiple para identificar estas especies empleando como muestra ADN de referencia, utilizando los juegos de cebadores complementarios a fragmentos de los genes de la proteína adhesiva de M. genitalium (MgPa, ARN ribosomal 16S de M. hominis, región espaciadora entre los genes del ARN ribosomal 16S y 23S de U. parvum, y de la región espaciadora adyacente al gen de la ureasa y específico para U. urealyticum, siendo un método específico y sensible. Resultados: Al analizar 34 muestras de exudado uretral, 27 correspondieron a la clase Mollicutes, obteniéndose 14,8% de positividad a M. genitalium, 18,5% a M. hominis, 11,1% a U. urealyticum y 3,7%. a U. parvum. Con este trabajo se realizó por primera vez el diagnóstico de M. genitalium, M. hominis, U. parvum y U. urealyticum en muestras uretrales de pacientes cubanos. Conclusión: Se recomienda incluir el diagnóstico de estas especies en un mayor número de pacientes cubanos con síntomas uretrales, para validar el método propuesto y conocer la relación de estos microorganismos con la UNG.

  15. First report of furuncular myiasis caused by the larva of botfly, Dermatobia hominis, in a Taiwanese traveler.

    Science.gov (United States)

    Hu, Je-Ming; Wang, Chih-Chien; Chao, Li-Lian; Lee, Chung-Shinn; Shih, Chien-Ming

    2013-03-01

    A case of furuncular myiasis was reported for the first time in a 29-year-old young Taiwanese traveler returning from an ecotourism in Peru. Furuncle-like lesions were observed on the top of his head and he complained of crawling sensations within his scalp. The invasive larva of botfly, Dermatobia hominis, was extruded from the furuncular lesion of the patient. Awareness of cutaneous myiasis for clinicians should be considered for a patient who has a furuncular lesion and has recently returned from a botfly-endemic area. PMID:23620844

  16. First report of furuncular myiasis caused by the larva of botfly, Dermatobia hominis, in a Taiwanese traveler.

    Science.gov (United States)

    Hu, Je-Ming; Wang, Chih-Chien; Chao, Li-Lian; Lee, Chung-Shinn; Shih, Chien-Ming

    2013-03-01

    A case of furuncular myiasis was reported for the first time in a 29-year-old young Taiwanese traveler returning from an ecotourism in Peru. Furuncle-like lesions were observed on the top of his head and he complained of crawling sensations within his scalp. The invasive larva of botfly, Dermatobia hominis, was extruded from the furuncular lesion of the patient. Awareness of cutaneous myiasis for clinicians should be considered for a patient who has a furuncular lesion and has recently returned from a botfly-endemic area.

  17. Dípteros fanídeos vetores de ovos de Dermatobia hominis em Campo Grande, Mato Grosso do Sul

    Directory of Open Access Journals (Sweden)

    Gomes Patrícia R.

    2002-01-01

    Full Text Available Determinou-se a importância epidemiológica de dípteros Fanniidae na infestação de mosca-do-berne, por meio da identificação das espécies presentes, da determinação daquelas utilizadas por Dermatobia hominis na veiculação de seus ovos, bem como, pelo conhecimento da dinâmica populacional das espécies mais abundantes. Foram utilizadas cinco armadilhas iscadas com fígado bovino cru deteriorado e colocadas em uma mata ciliar margeada por uma área de pastagem com presença constante de bovinos. O estudo foi desenvolvido em uma área da Embrapa Gado de Corte, em Campo Grande, Estado de Mato Grosso do Sul, Brasil, localizada a 20º27'S e 54º37'W. A captura dos insetos foi realizada semanalmente durante o período de 09/08/1999 a 03/08/2000. Foi capturado um total de 40.629 moscas da família Fanniidae, pertencendo a cinco espécies do gênero Fannia: F. pusio, F. heydenii, F. bahiensis e F. longipila, e uma a ser identificada. A espécie mais freqüente foi F. pusio, com 63,20% do total capturado, seguida de F. heydenii, com 28,82%. Somente 0,44% do total de fêmeas de F. heydenii (45 exemplares capturadas, principalmente nos meses de agosto e setembro, portavam ovos de D. hominis e o número médio, por indivíduo, foi de 15,98±7,13. Observaram-se ovos de D. hominis apenas na região abdominal dos vetores. F. heydenii predominou no período seco (maio a setembro e início do período chuvoso do ano (outubro e novembro. O número de exemplares portando ovos de D. hominis foi maior no final do período seco do ano, o que explica a alta incidência deste parasito em bovinos nos meses de setembro e outubro.

  18. Analysis of two genomes from the mitochondrion-like organelle of the intestinal parasite Blastocystis: complete sequences, gene content, and genome organization.

    Science.gov (United States)

    Pérez-Brocal, Vicente; Clark, C Graham

    2008-11-01

    Acquisition of mitochondria by the ancestor of all living eukaryotes represented a crucial milestone in the evolution of the eukaryotic cell. Nevertheless, a number of anaerobic unicellular eukaryotes have secondarily discarded certain mitochondrial features, leading to modified organelles such as hydrogenosomes and mitosomes via degenerative evolution. These mitochondrion-derived organelles have lost many of the typical characteristics of aerobic mitochondria, including certain metabolic pathways, morphological traits, and, in most cases, the organellar genome. So far, the evolutionary pathway leading from aerobic mitochondria to anaerobic degenerate organelles has remained unclear due to the lack of examples representing intermediate stages. The human parasitic stramenopile Blastocystis is a rare example of an anaerobic eukaryote with organelles that have retained some mitochondrial characteristics, including a genome, whereas they lack others, such as cytochromes. Here we report the sequence and comparative analysis of the organellar genome from two different Blastocystis isolates as well as a comparison to other genomes from stramenopile mitochondria. Analysis of the characteristics displayed by the unique Blastocystis organelle genome gives us an insight into the initial evolutionary steps that may have led from mitochondria to hydrogenosomes and mitosomes. PMID:18765437

  19. Morfología y respuesta de anticuerpos IgM e IgG anti-Blastocystis sp. en pacientes con síntomas gastrointestinales.

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    Emilia Elena Barrios

    2013-12-01

    Full Text Available Morphology and response of IgM and IgG anti - Blastocystis sp. in patients with gastrointestinal symptoms. It is important to define diagnostic criteria to elucidate the pathogenic role of Blastocystis sp . Morphology was assessed and diameter of the shapes, number of parasites per field and per gram of faeces and Blastocystis sp . Ig M and IgG antibodies was estimated by ELISA in subjects with constitutional symptoms an d healthy carriers. Seventy percent Blasctocystis sp positive samples were found, 52% from patients with symp toms such as flatulence 6 (24% , abdomi nal pain 5 (20 %, nausea 2 (8% , diarrhea 6 (24% and cons tipation 6 (24% . Ranges of parasites per field f ound more frequently in symptomatic patients were 0 - 3 (74% and 10 - 12 (26% , while 100% of asymptomatic patients had Blastocystis sp . in the range 0 - 3. Asymptomatic patients showed lower number of parasites per gram feces in the range of 0 - 3 and the 7 - 10 r ange with high parasitic load w as only observed in symptomatic . Less granular forms in both groups was found , in the range 0 - 3 in symptomatic patients : 85% vacuolar and 15% granular ; 76% vacuolar and 24 % granular in asymptomatic s . In the range 10 - 12, 98% v acuolar and 2% granular in symptomatic patients . The average diamet er of the vacuolar forms was 11. 9 ± 2 μm in s ymptomatic patients and 7.9 ± 3. 7 μm in asymptomatic s . In the granular form was 9.3 ± 2. 3 μm in symptomatic and 8.2 ± 0. 1 μm in asymp tomatics . Significant difference was found among vacuolar forms of both groups ( P = 0. 000026 , but not in the granular form (P = 0. 346 . Only 3. 5 % of s ymptomatic patients had IgG antibodies to Blastocystis sp. The m orphological study employed contributed to the diagn osis optimization while immunoenzymatic techniques were of low sensitivity.

  20. Treatment for intractable anemia with the traditional Chinese medicines Hominis Placenta and Cervi Cornus Colla (deer antler glue

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    Yasuyo Hijikata

    2009-05-01

    Full Text Available Yasuyo Hijikata1, Takashi Kano2, Lu Xi31Toyodo Hijikata Clinic, Osaka, Japan; 2Kano Clinic, Osaka city, Osaka, Japan; 3Traditional Chinese Medicine Institute, Si-chuan Province, ChinaObjective: Intractable anemia, such as aplastic anemia or that presumably associated with chronic herpes virus infections, sometimes require bone marrow transplant. We investigated the use of traditional Chinese medicine (TCM for the treatment of intractable anemia. Method: Placenta Hominis (PH, steam boiled and roasted, and Cervi Cornus Colla (deer antler glue has been used in China for hundreds of years to treat anemia. After consent was obtained, we prescribed these two materials for a 74-year-old female with aplastic anemia and a 26-year-old male with presumably a virus-induced anemia. Concomitant conventional therapy was continued in both patients as prescribed by their respective attending physicians. Conclusion: Conventional therapy with steroid hormones, immunosuppressive drugs, platelet and erythrocyte transfusions were not effective in these patients. In addition, both patients suffered from serious side effects. In two patients, ingestion of Placenta Hominis and Cervi Cornus Colla with TCM prescriptions increased the platelet and enhanced the hemoglobin concentration in several months of therapy accompanied by a dramatic improvement in quality of life. The addition to conventional therapy of PH and Cervi Cornus Colla, the latter of which is very easy to obtain, may be one of the potentially advantageous choices in case of otherwise intractable anemia.Keywords: placenta, antler glue, Cervi Cornus Colla, anemia, aplastic anemia

  1. Physical and genetic mapping of the genomes of five Mycoplasma hominis strains by pulsed-field gel electrophoresis

    DEFF Research Database (Denmark)

    Ladefoged, Søren; Christiansen, Gunna

    1992-01-01

    We present the complete maps of five Mycoplasma hominis genomes, including a detailed restriction map and the locations of a number of genetic loci. The restriction fragments were resolved by field inversion gel electrophoresis or by the contour-clamped homogeneous-electric-field system of pulsed......-field gel electrophoresis. All the ApaI, SmaI, BamHI, XhoI, and SalI restriction sites (total of 21 to 33 sites in each strain) were placed on the physical map, yielding an average resolution of 26 kb. The maps were constructed using three different approaches: (i) size determination of DNA fragments...... partially or completely cleaved with one or two restriction enzymes, (ii) hybridization analysis with purified restriction fragments and specific probes, and (iii) use of linking clones. A genetic map was constructed by hybridization with gene-specific probes for rpoA, rpoC, rrn, tuf, gyrB, hup, fts...

  2. HIGH PREVALENCE OF Blastocystis spp. INFECTION IN CHILDREN AND STAFF MEMBERS ATTENDING PUBLIC URBAN SCHOOLS IN SÃO PAULO STATE, BRAZIL.

    Science.gov (United States)

    Rebolla, Mayra Frozoni; Silva, Eliete Maria; Gomes, Jancarlo Ferreira; Falcão, Alexandre Xavier; Rebolla, Maria Vicentina Frozoni; Franco, Regina Maura Bueno

    2016-01-01

    After a gastroenteritis outbreak of unknown etiology in the municipality of Sebastião da Grama, São Paulo, Brazil, we conducted a parasitological survey to establish the epidemiological profile of enteroparasitosis in children and staff members attending the public urban schools in operation in town. The cross-sectional study evaluated 172 children aged 11 months to 6 years old and 33 staff members aged 19 to 58 years old. Overall, 96 (55.81%) children and 20 (60.61%) staff members were mono-parasitized, while 58 (33.72%) children and 4 (12.12%) workers were poly-parasitized. Protozoa (88.37%; 72.73%) was more prevalent than helminthes (3.48%; 0%) in children and staff members respectively.Blastocystis spp. was the most prevalent parasite in children (86.63%) and staff members (66.67%). The age of 1 year old or less was found to be associated with increased prevalence of giardiasis [OR = 13.04; 95%CI 2.89-58.91; p = 0.00] and public garbage collection was identified as a protective factor against intestinal helminth infections [OR = 0.06; 95%CI 0.00-0.79; p = 0.03]. Although most of the children tested positive for Blastocystis spp. and also presented clinical signs/symptoms (62.2%), this association was not statistically significant [OR = 1.35; 95%CI 0.53-3.44; p = 0.51]. Intestinal parasites still represent a public health concern and this study underscores the importance of further investigations to better understand the pathogenic role of Blastocystis spp. PMID:27074325

  3. Susceptibilities of Mycoplasma hominis, M. pneumoniae, and Ureaplasma urealyticum to GAR-936, Dalfopristin, Dirithromycin, Evernimicin, Gatifloxacin, Linezolid, Moxifloxacin, Quinupristin-Dalfopristin, and Telithromycin Compared to Their Susceptibilities to Reference Macrolides, Tetracyclines, and Quinolones

    OpenAIRE

    Kenny, George E.; Cartwright, Frank D.

    2001-01-01

    The susceptibilities of Mycoplasma hominis, Mycoplasma pneumoniae, and Ureaplasma urealyticum to eight new antimicrobial agents were determined by agar dilution. M. pneumoniae was susceptible to the new glycylcycline GAR-936 at 0.12 μg/ml and evernimicin at 4 μg/ml, but it was resistant to linezolid. It was most susceptible to dirithromycin, quinupristin-dalfopristin, telithromycin, reference macrolides, and josamycin. M. hominis was susceptible to linezolid, evernimicin, and GAR-936. It was ...

  4. Digital signage sobre IP

    OpenAIRE

    Casademont Filella, Albert

    2012-01-01

    Projecte que versa sobre el disseny i la implementació d'un sistema de Digital Signage (Cartelleria Digital) sobre IP, creant un gestor que permeti controlar de forma remota tot un conjunt de petits dispositius connectats a pantalles que emeten continguts multimèdia com vídeos, imatges, feeds rss...

  5. Pleiotropic effects of Blastocystis spp. Subtypes 4 and 7 on ligand-specific toll-like receptor signaling and NF-κB activation in a human monocyte cell line.

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    Joshua D W Teo

    Full Text Available Blastocystis spp. is a common enteric stramenopile parasite that colonizes the colon of hosts of a diverse array of species, including humans. It has been shown to compromise intestinal epithelial cell barrier integrity and mediate the production of pro-inflammatory cytokines and chemokines. Mucosal epithelial surfaces, including the intestinal epithelium, are increasingly recognized to perform a vital surveillance role in the context of innate immunity, through the expression of pathogen recognition receptors, such as Toll-like receptors (TLRs. In this study, we use the human TLR reporter monocytic cell line, THP1-Blue, which expresses all human TLRs, to investigate effects of Blastocystis on TLR activation, more specifically the activation of TLR-2, -4 and -5. We have observed that live Blastocystis spp. parasites and whole cell lysate (WCL alone do not activate TLRs in THP1-Blue. Live ST4-WR1 parasites inhibited LPS-mediated NF-κB activation in THP1-Blue. In contrast, ST7-B WCL and ST4-WR1 WCL induced pleiotropic modulation of ligand-specific TLR-2 and TLR-4 activation, with no significant effects on flagellin-mediated TLR-5 activation. Real time-qPCR analysis on SEAP reporter gene confirmed the augmenting effect of ST7-B on LPS-mediated NF-κB activation in THP1-Blue. Taken together, this is the first study to characterize interactions between Blastocystis spp. and host TLR activation using an in vitro reporter model.

  6. Evaluation of cellular retinoic acid binding protein 2 gene expression through the retinoic acid pathway by co-incubation of Blastocystis ST-1 with HT29 cells in vitro.

    Science.gov (United States)

    Liao, Chen-Chieh; Song, Eing-Ju; Chang, Tsuey-Yu; Lin, Wei-Chen; Liu, Hsiao-Sheng; Chen, Lih-Ren; Huang, Lynn L H; Shin, Jyh-Wei

    2016-05-01

    Blastocystis is a parasitic protist with a worldwide distribution that is commonly found in patients with colon and gastrointestinal pathological symptoms. Blastocystis infection has also commonly been reported in colorectal cancer and HIV/AIDS patients with gastrointestinal symptoms. To understand the pathway networks of gene regulation and the probable mechanisms influencing functions of HT-29 host cells in response to parasite infection, we examined the expression of 163 human oncogenes and kinases in human colon adenocarcinoma HT-29 cells co-incubated with Blastocystis by in-house cDNA microarray and PCR analysis. At least 10 genes were shown to be modified following Blastocystis co-incubation, including those with immunological, tumorigenesis, and antitumorigenesis functions. The expression of genes encoding cellular retinoic acid binding protein 2 (CRABP2) and proliferating cell nuclear antigen (PCNA) was markedly upregulated and downregulated, respectively. Reverse transcriptase-PCR validated the modified transcript expression of CRABP2 and other associated genes such as retinoic acid (RA)-related nuclear-receptor (RARα). Together, our data indicate that CRABP2, RARα, and PCNA expressions are involved in RA signaling regulatory networks that affect the growth, proliferation, and inflammation of HT-29 cells. PMID:26911149

  7. Analysis of 0.5-kilobase-pair repeats in the Mycoplasma hominis lmp gene system and identification of gene products

    DEFF Research Database (Denmark)

    Ladefoged, Søren; Jensen, Lise Torp; Brock, B;

    1996-01-01

    Mycoplasma hominis, an opportunistic pathogenic bacterium of humans, has a small genome of 700 kb. Despite this, multiple copies of gene sequences with similarities to the structural gene (lmp1) of a 135-kDa surface-located membrane protein (Lmp1) have been identified on the genome of M. hominis PG...... repeats with 51 to 90% similarity to 10 similar repeats found in the lmp1-lmp2 region. The 0.5-kb DNA repeats thus comprised about 1% of the entire genome. In both regions, a base change in one of the repeats gave rise to a stop codon, and thereby lmp2 and lmp4 occurred. By PCR amplification of reverse...

  8. Isolation and characterization of two novel bacteria Afipia cberi and Mesorhizobium hominis from blood of a patient afflicted with fatal pulmonary illness.

    Directory of Open Access Journals (Sweden)

    Shyh-Ching Lo

    Full Text Available We recently isolated and discovered new Bradyrhizobiaceae microbes from the cryopreserved culture broth of blood samples from 3 patients with poorly defined illnesses using modified SP4 media and culture conditions coupled with genomic sequencing. Using a similar protocol, we studied a previously cryopreserved culture broth of blood sample from a patient who had succumbed to an acute onset of fulminant pulmonary illness. We report that two phases of microbial growth were observed in the re-initiated culture. Biochemical and genomic characterization revealed microbes isolated from the first phase of growth were new Afipia species of Bradyrhizobiaceae, tentatively named A. cberi with a ~ 5 MB chromosome that was different from those of all previously known Afipia microbes including the newly discovered A. septicemium. The microbes isolated from the second phase of growth were prominent sugar assimilators, novel Phyllobacteriaceae, phylogenetically most closely related to Mesorhizobium and tentatively named M. hominis with a ~ 5.5 MB chromosome. All A. cberi isolates carry a circular ~ 140 KB plasmid. Some M. hominis isolates possess a circular ~ 412 KB plasmid that can be lost in prolonged culture or passage. No antibiotics resistant genes could be identified in both of the A. cberi and M. hominis plasmids. Antibiotic susceptibility studies using broth culture systems revealed isolates of A. cberi could be sensitive to some antibiotics, but all isolates of M. hominis were resistant to essentially all tested antibiotics. However, the cell-free antibiotics susceptibility test results may not be applicable to clinical treatment against the microbes that are known to be capable of intracellular growth. It remains to be determined if the 2 previously unknown Rhizobiales were indeed pathogenic and played a role in the pulmonary disease process in this patient. Specific probes and methods will be developed to re-examine the diseased lungs from patient

  9. OppA, the ecto-ATPase of Mycoplasma hominis induces ATP release and cell death in HeLa cells

    Directory of Open Access Journals (Sweden)

    Henrich Birgit

    2008-04-01

    Full Text Available Abstract Background In the facultative human pathogen Mycoplasma hominis, which belongs to the cell wall-less Mollicutes, the surface-localised substrate-binding domain OppA of the oligopeptide permease was characterised as the main ecto-ATPase. Results With the idea that extra-cellular ATP could only be provided by the infected host cells we analysed the ATP release of HeLa cells after incubation with different preparations of Mycoplasma hominis: intact bacterial cells, the membrane fraction with or without OppA, recombinant OppA as well as an ATPase-deficient OppA mutant. Release of ATP into the supernatant of the HeLa cells was primarily determined in all samples lacking ecto-ATPase activity of OppA. In the presence of the ATPase inhibitor DIDS the amount of ATP in the OppA-containing samples increased. This increase was maximal after incubation with fractions containing OppA protein indicating that OppA is involved in ATP release and subsequent hydrolysis. Real-time PCR analyses revealed that the proliferation of HeLa cells is reduced after infection with M. hominis and flow cytometry experiments established that OppA induces greater apoptosis than necrosis of HeLa cells whereas the preservation of ecto-ATPase activity of OppA induces apoptosis. Conclusion The OppA induced ATP-release and -hydrolysis induced cell death of M. hominis infected HeLa cells was predominantly due to apoptosis rather than necrosis. Future work will elucidate whether the induction of apoptosis is indispensable for survival of these non-invasive pathogen.

  10. Ultrafiltros sobre ω.

    OpenAIRE

    Mancilla Hernández, Salvador

    2012-01-01

    El objetivo del presente trabajo estudiar los ideales y filtros sobre conjuntos numerables, los que comúnmente conocemos como filtros sobre ω o ideales sobre ω. Dentro de estos, nos enfocaremos en la clase de I-ultrafiltros los cuales fueron estudiados por James Baumgartner en [11]. Y dentro de esta clase de ultrafiltros, nos concentraremos cuando I es {A ⊆ ω : n∈A g(n) < ∞}, también conocido como ideal sumable con respecto a g, donde g es una sucesión de números reales no negativ...

  11. Effects of Hominis Placenta Aqua-acupuncture on Kidney and Liver Intoxicated by HgCI2 in Rats

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    Lee, Sang-Keel

    2000-12-01

    Full Text Available Objective: This study was perfomled to examine the therapeutic effect of aqua-acupuncture solution of Hominis Placenta(HP on kidney and liver intoxicated by HgCl2 in rats. Methods: 10% and 25% HP aqua-acupuncture were carried out everyday for 8 days on corresponding bilateral loci of Shinsu(BL23 and Kansu(BL18, respectively, after mercuric chloride intoxication in rats. Thereafter BUN, creatinine, GOT, GPT, ALP, -GT, albumin and total bilirubin were measured before intoxication, and at the 4th and the 8th experimental day. Histopathological and immunochemical observation were also carried out. Results: 1. It showed significant decreases of BUN in the group of 10% HP aqua-acupuncture into Shinsu on the 4th experimental day as compared with the control group. 2. It showed significant decreases of creatinine in the group of 10% HP aqua-acupuncture into Shinsu on the 4th and the 8th experimental days as compared with the control group. 3. There were not any significant changes of GOT, GPT, ALP, γ-GT, albumin and total bilirubin in the HP aqua-acupuncture groups compared with the control group. 4. By the histopathological observations on kidney under a light microscope, all the 10% and 25% HP aqua-acupuncture into Shinsu showed the preventive effect on tubulo-interstitial necrosis and muItifocal calcification in tubular lumen respectively compared with the control group. 5. By the histopathological observations on liver under a light mIcroscope, the groups 10% and 25% HP aqua-acupuncture into Kansu did not show any significant changes in the liver compared with the control group. 6. By the immunochemical analysis of heat shock protein(hsp and glucose-regulated protein(grp in rat renal cortex, the expressions of hsp70 and grp78 were decreased in the and HP aqua-acupuncture into Shinsu respectively compared with the control group. Conclusion: These results suggest that Hominis Placenta aqua-acupuncture have an effect on prevention and protection of

  12. Stimulative Effects of Hominis Placental Pharmacopuncture Solution Combined with Zinc-oxide Nanoparticles on RAW 264.7 Cells

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    Hong Tae-Keun

    2012-09-01

    Full Text Available Objectives: The purpose of this study is to examine whether Hominis Placental pharmacopuncture solution (HPPS combined with zinc-oxide nanoparticles (ZnO NP activates RAW 264.7 cells. Methods: We soaked ZnO nanoparticles in the Hominis Placenta pharmacopuncture solution, thereby making a combined form (ZnO NP HPPS. The effect of ZnO NP HPPS on the intracellular reactive oxygen species (ROS production was measured by 2’, 7’-dichlorofluorescin diacetate (DCFH-DA assay. The effect of ZnO NP HPPS on NF-κ B was measured by using a luciferase assay. The effect of ZnO NP HPPS on the cytokine expression was assessed by semi-quantitative reverse transcriptase polymerase chain reaction (RT-PCR. The cellular uptake of ZnO NP HPPS was measured by using a flow cytometric analysis, and cellular structural alterations were analyzed by using transmission electron microscopy (TEM. Results: Neither the HPPS nor the ZnO NPs induced intracellular ROS production in RAW 264.7 cells. Neither of the materials activated NF-κ B or it’s dependent genes, such as TNF-α, IL-1, and MCP-1. However, ZnO NP HPPS, the combined form of ZnO NPs and HPPS, did induce the intracellular ROS production, as well as prominently activating NF-κ B and it’s dependent genes. Also, compared to ZnO NPs, it effectively increa-sed the uptake by RAW 264.7 cells. In addition, cellular structural alterations were observed in groups treated with ZnO NP HPPS. Conclusions: Neither ZnO NP nor HPPS activated RAW 264.7 cells, which is likely due to a low cellular uptake. The ZnO NP HPPS, however, significantly activated NF-κ B and up-regulated its dependent genes such as TNF-α, IL-1, and MCP-1. ZnO NP HPPS was also more easily taken into the RAW 264.7 cells than either ZnO NP or HPPS.

  13. Detection of Cryptosporidium hominis and novel Cryptosporidium bat genotypes in wild and captive Pteropus hosts in Australia.

    Science.gov (United States)

    Schiller, Sabine Eva; Webster, Koa Narelle; Power, Michelle

    2016-10-01

    Spillover of zoonotic pathogens from wildlife to humans has been identified as a primary threat to global health. In contrast, the process of reverse pathogen transmission (zooanthroponosis), whereby pathogens move from humans into wildlife species is still largely unexplored. Globally, increasing urbanisation and habitat loss are driving many wildlife species into urban and regional centres. In Australia, large numbers of flying foxes now live in close proximity to humans, increasing the risk of zooanthroponosis. The protozoan parasite Cryptosporidium is an emerging zoonotic parasite that infects a wide range of vertebrates yet there are limited studies on transmission potential of Cryptosporidium between humans and urban wildlife. To explore the presence of zooanthroponosis in flying foxes in Australia the occurrence and diversity of Cryptosporidium was investigated in urbanised wild and captive flying foxes. PCR screening of faecal samples (n=281) from seven wild sites and two captive facilities identified the presence of Cryptosporidium in 3.2% (95% CI 1.5% to 6.0%) of faecal samples. In faecal samples from wild sites Cryptosporidium occurrence was 1.7% (95% CI 0.3% to 4.8%) versus 5.9% (95% CI 2.2% to 12.4%) in samples from captive individuals, with no significant difference between captive and wild sites (p=0.077). Multilocus sequencing using three loci, 18s rDNA, actin and gp60 was used to identify Cryptosporidium in flying fox species. The host specific Cryptosporidium hominis was identified in faecal samples from two captive flying foxes, and one of these samples was confirmed as C. hominis at both actin and gp60. Sequencing of the 18s rDNA also revealed four novel Cryptosporidium genotypes in wild and captive individuals, actin and gp60 amplification and sequencing were unreliable for all four novel genotypes. These novel genotypes have been designated Cryptosporidium bat genotypes VIII-XI. This first report of Cryptosporidium spp. in Australian flying

  14. Desenvolvimento pós-embrionário do intestino médio de Dermatobia hominis (Linnaeus Jr. (Diptera, Cuterebridae Post-embryonic development of Dermatobia hominis (Linnaeus Jr. (Diptera, Cuterebridae midgut

    Directory of Open Access Journals (Sweden)

    Edy de Lello

    2001-03-01

    Full Text Available Dermatobia hominis (Linnaeus, 1781 midgut is internally lined by an epithelium of polytenic cells, some low others prismatic with well developed brush border. Their apical portion are enlarged by secretory vesicles, forming button-like structures that are pinched off to the lumen, some accompained by the nucleus characterizing apocrine and holocrine secretions. This epithelium is gradually renewed by small, non polytenic regenerative cells, found scattered at its basal portion. At the end of the third instar the metamorphosis begins. The epithelial cells present signs of degeneration and at the first day of pupation the regenerative cells increase in number. By the 5th day of pupation these regenerative cells, besides being increased in number, differentiate themselves into two layers: one similar to the dense conective tissue that sustainning the larval epithelium is pinched off to the midgut lumen forming the "yellow bodies"; the other, develops right under it as the imaginal epitelium. The disorganized muscles bundles of the midgut wall, are invaded by phagocytes. At the end of pupation the midgut has a low prismatic epithelium with brush-border. In the adult, the torax portion of the midgut has prismatic homogeneously basophilic epithelium while in the abdominal portion the epithelium is made of high prismatic cells full of small vacuoles. The larval midgut epithelium suffers programmed cell death non compatible with apoptose. During the metamorphosis the midgut lenght diminishes from 31mm in the larva to 14mm in the adult.

  15. Desenvolvimento pós-embrionário do intestino anterior de Dermatobia hominis (Linnaeus Jr. (Diptera, Cuterebridae Post-embryonic development of Dermatobia hominis (Linnaeus Jr. (Diptera, Cuterebridae foregut

    Directory of Open Access Journals (Sweden)

    Ana Maria Vieira

    2001-03-01

    Full Text Available Foregut in D. hominis (Linnaeus Jr., 1781 as the majority of the larval Diptera somatic tissue, is made up of polytenic cells, and grows at the expenses of the polytenization of its nuclei followed by the increase in size of each cell. The oesophagus, of ectodermic origem, is interiorly covered by a chitinous squamous epithelium that rests upon a very thin basal lamina. This sheet is surrounded by thick muscle bundles. The oesophagus intussuscepts the midgut forming the cardia. The cardia, with three epithelial layers: two internal ones, of ectodermal origin and one external of endodermic origin. At the anterior portion of the cardia, between these two types of epithelium, there is a cluster of small, non polytenic cells, forming the imaginal disk of the foregut. Metamoiphosis begins at the end of the larval period with signs of nuclear degeneration of all the polytenic cells, as well as the increase in number of the imaginal disk ones. The oesophagic portion intussuscepted into the cardia, everts; its cells suffer apoptosis and are replaced by the new cells growing from the imaginal disk. The external layer cells also degenerate and are pinched off into the lumen of the very anterior portion of the midgut. The newly formed oesophagus intussuscepts "de novo" to form the two internal layers of the adult cardia. At the same time the midgut regenerative cells grow anteriorly to form the new external layer of the adult cardia.

  16. PCR Research of Mycoplasma hominis in Trichomonas vaginalis Cells of Sichuan Province%四川地区阴道毛滴虫内人型支原体的PCR检测

    Institute of Scientific and Technical Information of China (English)

    朱晓燕; 王雅静; 毕世樑; 张仁刚

    2009-01-01

    20 isolates of Trichomonas vaginalis were collected from clinical patients in hospital. After pure cultivation, genomic DNA of T. vaginalis was extracted. A pair of specific 16S rDNA primers was designed based on the sequence of Mycoplasma hominis. M. hominis in T. vaginalis cells were detected by PCR. Altogether 20 isolates of T. vaginalis were collected, 13 out of 20 T. vaginalis isolates harboured M. hominis, and the symbiosis ratio between T. vaginalis and M. hominis was up to 65%. It suggested that the symbiotic relationship between T. vaginalis and M. hominis occurred commonly in Sichuan, China.%从临床上分离获得20株阴道毛滴虫虫株,经纯化培养后,提取基因组DNA.以人型支原体16S rDNA序列设计特异性引物,利用PCR技术检测阴道毛滴虫内的人型支原体,结果有13株为人型支原体阳性,感染率为65%,表明阴道毛滴虫与人型支原体的共生关系在中国四川具有普遍性.

  17. Todo sobre el neobarroco

    Directory of Open Access Journals (Sweden)

    Arturo Carrera

    2007-07-01

    Full Text Available Lo que hemos escuchado y atendido hasta aquí, hoy, ayer, sobre todo en la propuesta poética esbozada por Sergio Raimondi en su ensayo sobre Aldo Oliva, demuestra que las singularidades, su pasión, anulan subrepticiamente las nominaciones.Anoche en la televisión una orquesta sinfónica y un coro numeroso. Súbitamente, me hubiera gustado ser uno de esos intérpretes solitarios para sí, a pesar de la armonía, anónimos, a pesar del brillo sonoro… un violín, un triángulo… apenas… tres grillos… como ...

  18. Inferencias sobre Grafos

    OpenAIRE

    Sira M. Allende; Carlos N. Bouza

    2002-01-01

    El estudio de un juego puede ser modelado asumiendo que solo algunas partidas son observadas. Entonces el árbol del juego debe ser estimado utilizando información muestral. Similarmente ocurre al obtener información sobre el comportamiento de las decisiones tomadas por individuos muestreados sobre un árbol de decisión teórico. Al considerar una medida de probabilidad que caracterice el comportamiento de redes aleatorias se puede obtener un estimado del árbol asociado. Este problema es el anal...

  19. Mitos sobre agroecologia.

    OpenAIRE

    Canuto, João Carlos

    2011-01-01

    O presente ensaio vem a discutir alguns aspectos do discurso sobre a Agroecologia, procurando evidenciar contradições do senso comum, advindas seja de parte da comunidade leiga, dos meios de comunicação ou da comunidade científica. Coloca em debate alguns mitos sobre os sistemas agroecológicos, tais como: que sejam tecnologicamente retrógrados, de baixa produtividade, exigentes em mão-de-obra, economicamente inviáveis e “puramente ideológicos”.

  20. Protozoa Intestinalis

    OpenAIRE

    Hemma Yulfi

    2006-01-01

    Protozoa intestinal terdiri atas amebae, flagellata, dan cilliata. Termasuk amebae intestinal adalah Entamoeba histolytica, Entamoeba coli, Entamoeba hartmanni, Endolimax nana, Iodamoeba butschlii, Dientamoeba fragilis, dan Blastocystis hominis, oleh Hemma Yulfi 06001187

  1. The genome of the obligate intracellular parasite Trachipleistophora hominis: new insights into microsporidian genome dynamics and reductive evolution.

    Directory of Open Access Journals (Sweden)

    Eva Heinz

    Full Text Available The dynamics of reductive genome evolution for eukaryotes living inside other eukaryotic cells are poorly understood compared to well-studied model systems involving obligate intracellular bacteria. Here we present 8.5 Mb of sequence from the genome of the microsporidian Trachipleistophora hominis, isolated from an HIV/AIDS patient, which is an outgroup to the smaller compacted-genome species that primarily inform ideas of evolutionary mode for these enormously successful obligate intracellular parasites. Our data provide detailed information on the gene content, genome architecture and intergenic regions of a larger microsporidian genome, while comparative analyses allowed us to infer genomic features and metabolism of the common ancestor of the species investigated. Gene length reduction and massive loss of metabolic capacity in the common ancestor was accompanied by the evolution of novel microsporidian-specific protein families, whose conservation among microsporidians, against a background of reductive evolution, suggests they may have important functions in their parasitic lifestyle. The ancestor had already lost many metabolic pathways but retained glycolysis and the pentose phosphate pathway to provide cytosolic ATP and reduced coenzymes, and it had a minimal mitochondrion (mitosome making Fe-S clusters but not ATP. It possessed bacterial-like nucleotide transport proteins as a key innovation for stealing host-generated ATP, the machinery for RNAi, key elements of the early secretory pathway, canonical eukaryotic as well as microsporidian-specific regulatory elements, a diversity of repetitive and transposable elements, and relatively low average gene density. Microsporidian genome evolution thus appears to have proceeded in at least two major steps: an ancestral remodelling of the proteome upon transition to intracellular parasitism that involved reduction but also selective expansion, followed by a secondary compaction of genome

  2. DNA sequencing reveals limited heterogeneity in the 16S rRNA gene from the rrnB operon among five Mycoplasma hominis isolates

    DEFF Research Database (Denmark)

    Mygind, T; Birkelund, Svend; Christiansen, Gunna

    1998-01-01

    To investigate the intraspecies heterogeneity within the 16S rRNA gene of Mycoplasma hominis, five isolates with diverse antigenic profiles, variable/identical P120 hypervariable domains, and different 16S rRNA gene RFLP patterns were analysed. The 16S rRNA gene from the rrnB operon was amplified...... by PCR and the PCR products were sequenced. Three isolates had identical 16S rRNA sequences and two isolates had sequences that differed from the others by only one nucleotide....

  3. Detección de parásitos intestinales en agua y alimentos de Trujillo, Perú

    OpenAIRE

    Gregorio Pérez-Cordón; María J. Rosales; Renzo A. Valdez; Franklin Vargas-Vásquez; Ofelia Cordova

    2008-01-01

    Detectamos distintas especies de parásitos intestinales, tanto protozoos como helmintos, presentes en muestras de agua provenientes de acequias y pozos (Giardia lamblia, Blastocystis hominis, Entamoeba coli, Cyclospora cayetanensis, Cryptosporidium spp. y Balantidium coli), así como en alimentos crudos y cocidos (Giardia lamblia, Cyclospora cayetanensis., Endolimax nana, Iodamoeba butschlii y Blastocystis hominis Fasciola hepatica y Ascaris lumbricoides) recolectadas en varios distritos de la...

  4. Detección de parásitos intestinales en agua y alimentos de Trujillo, Perú

    Directory of Open Access Journals (Sweden)

    Gregorio Pérez-Cordón

    2008-01-01

    Full Text Available Detectamos distintas especies de parásitos intestinales, tanto protozoos como helmintos, presentes en muestras de agua provenientes de acequias y pozos (Giardia lamblia, Blastocystis hominis, Entamoeba coli, Cyclospora cayetanensis, Cryptosporidium spp. y Balantidium coli, así como en alimentos crudos y cocidos (Giardia lamblia, Cyclospora cayetanensis., Endolimax nana, Iodamoeba butschlii y Blastocystis hominis Fasciola hepatica y Ascaris lumbricoides recolectadas en varios distritos de la provincia de Trujillo, Perú.

  5. Draft Genome Sequences of Microbacterium hominis LCDC-84-0209T Isolated from a Human Lung Aspirate and Microbacterium laevaniformans LCDC 91-0039 Isolated from a Human Blood Culture

    Science.gov (United States)

    Bernier, Anne-Marie

    2016-01-01

    Draft genomes for Microbacterium hominis 84-0209T and M. laevaniformans 91-0039 were studied. Genome sizes (bps, [G+C contents]) were 3,506,522 (70.96%) and 2,999,965 (69.51%), respectively. Annotation revealed: (M. hominis) three rRNA sequences, 45 tRNA genes, and 3,218 coding sequences; (M. laevaniformans) three rRNA sequences, 49 tRNA genes, and 2,874 coding sequences. PMID:27635007

  6. Draft Genome Sequences of Microbacterium hominis LCDC-84-0209T Isolated from a Human Lung Aspirate and Microbacterium laevaniformans LCDC 91-0039 Isolated from a Human Blood Culture.

    Science.gov (United States)

    Bernier, Anne-Marie; Bernard, Kathryn

    2016-01-01

    Draft genomes for Microbacterium hominis 84-0209(T) and M. laevaniformans 91-0039 were studied. Genome sizes (bps, [G+C contents]) were 3,506,522 (70.96%) and 2,999,965 (69.51%), respectively. Annotation revealed: (M. hominis) three rRNA sequences, 45 tRNA genes, and 3,218 coding sequences; (M. laevaniformans) three rRNA sequences, 49 tRNA genes, and 2,874 coding sequences. PMID:27635007

  7. Draft Genome Sequences of Microbacterium hominis LCDC-84-0209T Isolated from a Human Lung Aspirate and Microbacterium laevaniformans LCDC 91-0039 Isolated from a Human Blood Culture.

    Science.gov (United States)

    Bernier, Anne-Marie; Bernard, Kathryn

    2016-01-01

    Draft genomes for Microbacterium hominis 84-0209(T) and M. laevaniformans 91-0039 were studied. Genome sizes (bps, [G+C contents]) were 3,506,522 (70.96%) and 2,999,965 (69.51%), respectively. Annotation revealed: (M. hominis) three rRNA sequences, 45 tRNA genes, and 3,218 coding sequences; (M. laevaniformans) three rRNA sequences, 49 tRNA genes, and 2,874 coding sequences.

  8. Dicty_cDB: Contig-U05250-1 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available chromosom... 44 8.5 1 ( EF494740 ) Blastocystis sp. NandII mitochondrion, complete g... 44 8.5 1 ( CT963106... WIN1126.C21_N19 Muscat Hamburg pre-veraison berry... 44 8.5 1 ( EC641976 ) BHE00001891 Blastocystis hominis

  9. Inferencias sobre Grafos

    Directory of Open Access Journals (Sweden)

    Sira M. Allende

    2002-05-01

    Full Text Available El estudio de un juego puede ser modelado asumiendo que solo algunas partidas son observadas. Entonces el árbol del juego debe ser estimado utilizando información muestral. Similarmente ocurre al obtener información sobre el comportamiento de las decisiones tomadas por individuos muestreados sobre un árbol de decisión teórico. Al considerar una medida de probabilidad que caracterice el comportamiento de redes aleatorias se puede obtener un estimado del árbol asociado. Este problema es el analizado en este trabajo. Algunos resultados experimentales obtenidos usando Recocido Simulado ilustran el procedimiento. Su papel en el estudio de modelos económicos y de mercadeo es discutido.

  10. A Study on Diagnosis of Mycoplasma Hominis in The Urogenital Tract By Nested Polymerase Chain Reaction with First Void Urine in Men

    Institute of Scientific and Technical Information of China (English)

    徐斌; 温泉; 刘洋; 张林

    2001-01-01

    Objectives: To evaluate the efficacy of nested polymerase chain reaction (PCR) with first void urine (FVU) for the diagnosis of Mycoplasma hominis in male patients.Methods: Matched FVU specimens and urethral swabs were collected from 194 male patients with Nongonococcal Urethritis and tested by nested PCR and cell culture. Cell culture was used as a gold standard for evaluating other assay techniques.Results: For FVU nested PCR assay and FVU cell culture,our results showed that the sensitivity was 100% and 93.3%;specificity was 97.0% and 98.2%; positive predictive value (PPV) was 85.7% and 90.3%, negative predictive value (NPV)was 100% and 98.8%, respectively. The total consistency between the two techniques was 97.4 %.Conclusions: For the diagnosis of Mycoplasma hominis in men, nested PCR detecting FVU is a highly sensitive and specific method. First void urine can replace swab culture or PCR in terms of acceptability and feasibility.

  11. Estudios sobre Borges

    OpenAIRE

    Facultad de Humanidades y Ciencias de la Educación

    1991-01-01

    Contenidos de la obra: De Barthes a Pierre Menard | José Luis De Diego Pierre Menard, autor del Quijote: De la poligrafía al fraude | Andrea Cucatto Homenaje a Roberto Arlt: La otra cara de la moneda | Fabio Espósito Emma a través del espejo: Una lectura de Emma Zunz | Graciela Beatriz Goldchluk Sobre el concepto de verdad en Borges | Sergio Pastormerlo

  12. 人芽囊原虫感染者外周血T细胞亚群及膜白介素-2受体的检测%Detection of mIL-2R and T lymphocyte subsets in PBMC of the patients infected by Blastocystis hominis

    Institute of Scientific and Technical Information of China (English)

    崔玉宝; 陈琳; 李朝品

    2003-01-01

    目的探讨人芽囊原虫感染者外周血T细胞亚群及膜白介素-2受体(mIL-2R)的变化.方法采用生物素-链霉亲和素(BSA)法对卵囊阳性患者进行外周血T细胞亚群、mIL-2R检测.结果人芽囊原虫卵囊阳性者CD3+、CD4+、CD8+和CD4+/CD8+阳性百分率分别为(65.83±6.55)%、(43.55±6.10)%、(28.43±4.32)%和1.58±0.32,静息期与诱导期mIL-2R表达水平分别为(2.92±1.06)%和(33.45±2.31)%;人芽囊原虫卵囊阴性者CD3+、CD4+、CDR+和CD4+/CD8+阳性百分率分别为(55.87±7.23)%、(39.26±6.43)%、(30.04±5.67)%和1.36±0.41,静息期与诱导期mIL-2R表达水平分别为(4.31±1.47)%和(35.41±3.12)%,两者相比,差异均有显著性(P<0.05~0.01).结论人芽囊原虫感染与细胞免疫功能密切相关,T细胞亚群、mIL-2R在抗人芽囊原虫感染中起重要作用.

  13. 人芽囊原虫病患者肠粘膜损伤及其粘膜细胞因子的测定%PATHOLOGIC OBSERVATION OF INTESTINAL MUCOSAL LESION AND DETECTION OF CYTOKINE LEVEL IN THE PATIENTS WITH BLASTOCYSTIS HOMINIS INFECTION

    Institute of Scientific and Technical Information of China (English)

    金群馨; 唐国都; 俞开敏

    2005-01-01

    目的探讨人芽囊原虫感染引起的肠粘膜病变及其分泌细胞因子IL-8、IL-18、GM-CSF水平变化的病理意义.方法经粪便检查确诊为人芽囊原虫感染的30例患者做电子肠镜检查,取病变粘膜做病理检查.用ELISA法检测人芽囊原虫感染者和30例正常对照者肠粘膜组织匀浆中的IL-8、IL-18和GM-CSF水平. 结果电子肠镜检查人芽囊原虫感染者均出现肠道病理改变,病变部位主要在左半结肠、直肠,病变程度与人芽囊原虫感染度呈正相关(rs =0.853 8,P<0.001);病理切片检查多数粘膜呈慢性炎症或溃疡病变.患者肠粘膜组织匀浆中IL-8、IL-18、GM-CSF水平均显著高于正常人,且随人芽囊原虫感染度的加重逐渐升高. 结论人芽囊原虫感染能引起患者肠粘膜病理改变和细胞因子IL-8、IL-18、GM-CSF水平的升高,且与感染程度相关.人芽囊原虫感染诱导并调节肠上皮细胞的免疫反应,细胞因子可能起重要作用.

  14. 鸦胆子、黄连、白头翁和槟榔对人芽囊原虫体外杀虫作用的比较%In Vitro Antiprotozoal Effects of Brucea javanica,Coptis chinensis, Radix pulsatillae and Arecae on Blastocystis hominis

    Institute of Scientific and Technical Information of China (English)

    张旭; 乔继英; 张荣; 魏志超; 蔡娟

    2007-01-01

    目的 通过比较鸦胆子、黄连、白头翁和槟榔对人芽囊原虫的体外杀虫作用,筛选出对人芽囊原虫有效的中药及浓度.方法 分别于加药24 h和72 h后对细胞进行计数,来评价药物的作用效果.结果 鸦胆子的最适浓度为1 600~3 200 μg/ml;黄连的最适浓度为6 400 μg/ml;白头翁不能完全杀死虫体,其最低抑虫浓度为800 μg/ml;槟榔无效.结论 鸦胆子、黄连、白头翁和槟榔是传统的治疗肠道感染和抗肠道寄生虫的中草药.通过比较药物最适浓度和最低抑虫浓度,发现鸦胆子的杀虫效果强于黄连;白头翁可抑制虫体繁殖;槟榔对人芽囊原虫无抑制和杀灭作用.

  15. Informe APEI sobre movilidad

    OpenAIRE

    Arroyo Vázquez, Natalia

    2011-01-01

    Este informe incluye una amplia aproximación al concepto de movilidad, desde un punto de vista introductorio, que nunca antes en nuestro ámbito había abarcado todos los aspectos que tienen que ver con él, desde cuestiones técnicas como los dispositivos móviles, sistemas operativos y navegadores, conectividad y estándares —cuya intención es poder comprender todos aquellos aspectos relacionados con la movilidad y que tienen una incidencia directa sobre los contenidos— hasta otras más relacionad...

  16. Apuntes sobre liderazgo

    OpenAIRE

    Contreras, Manuel E.

    2010-01-01

    Este trabajo presenta una breve síntesis de las principales teorías de liderazgo y se centra sobre el enfoque de liderazgo adaptativo de Ronald Heifetz. El documento originalmente fue desarrollado para las sesiones de educación a distancia de INDES. Ha sido revisado para usar en otros ámbitos de capacitación y ha sido utilizado en sesiones presenciales llevadas a cabo por INDES, como por otras entidades en universidades y centros de capacitación de America Latina.

  17. Sobre el razonamiento judicial

    OpenAIRE

    Asís Roig, Rafael de

    1998-01-01

    El trabajo elabora modelos a través de los cuáles es posible reconstruir la argumentación judicial plasmada en las sentencias y, a la vez, hacer explícitas las reglas que sirven de justificación a sus decisiones, y el marco normativo utilizado como referencia. El estudio analiza tanto los pronunciamientos sobre hechos como los que se refieren a la calificación jurídica, en principio, desde una perspectiva descriptiva. Ahora bien, también se llevan a cabo reflexiones y propuestas que van más a...

  18. Puente sobre el Rin

    Directory of Open Access Journals (Sweden)

    Gortz, Wilhelm

    1961-12-01

    Full Text Available Del total de 35 proyectos presentados al concurso del puente para carretera sobre el Rin, entre Duisburg-Ruhrort y Homberg, sólo dos proposiciones de la Demag fueron consideradas: Una con tablero suspendido por vientos que parten de dos torres, y otra, con suspensión por cables flexibles que se apoyan en dos torres y anclaje en la misma estructura. Por razones netamente estéticas fue elegida la propuesta Demag de puente suspendido por cables, aunque la solución con vientos hubiera sido la más económica, teniendo en cuenta las condiciones existentes.

  19. Sobre la tragedia griega

    Directory of Open Access Journals (Sweden)

    Mauricio Vélez Upegui

    2015-01-01

    Full Text Available Luego de caracterizar la tragedia griega clásica como expresión de un arte ciudadano que participa por igual de un carácter festivo, cierto régimen discursivo y una clara inscripción físico-espacial, el texto se demora en considerar tres aspectos fundamentales de esta forma dramática inventada por los griegos, a saber: una exposición sobre las fuentes orales míticas de las que los autores echan mano para componer la historia de cada una de las piezas que son llevadas a escena al amparo de un espíritu agonal; una explicación del funcionamiento formal de la tragedia, basado en la alternancia de partes cantadas (coro y partes recitadas (héroe; y, finalmente, una acotación sobre el sentido –interno y externo– que la acción dramática posee, en tanto elemento articulador de la trama.

  20. Sobre historia mundial hoy

    Directory of Open Access Journals (Sweden)

    Vera Weiler

    2010-08-01

    Full Text Available Los procesos de globalización son una realidad; su enorme impacto sobre la vida humana los ha convertido en un tema recurrente sobre el que se escribe y se comenta a diario también en Colombia. Los libros que de ellos tratan, ante todo los que 10 hacen en tono crítico, baten records en la industria editorial en todo el mundo. Las expectativas que acerca del futuro abrigan cientos de millones de personas se relacionan con 10 que ellas esperan, para bien o para mal, de la globalización. Las posturas que los gobernantes ocupan al respecto en el mundo son, cuando menos de aceptación, generalmente de activa participación en pos de la globalización. Por un lado, crece la preocupación y se multiplican las protestas; por el otro, dominan los razonamientos de los especialistas en materia de maximización de los rendimientos de los capitales que presentan la globalización, al estilo que se viene imponiendo, como el camino ineludible del gobierno universal de la eficiencia. ¿Tienen que decir algo los historiadores frente a todo esto?.

  1. Ocorrência de vetores biológicos da Dermatobia hominis (L.Jr., 1781 (Díptera: Cuterebridae, capturados com armadilha magoom na região metalúrgica do estado de Minas Gerais, Brasil Ocurrence of biological vectors of Dermatobia hominis (L. Jr.,1781(Diptera:Cuterebridae, captured by magoom trap in the matallurgic region, Minas Gerais, Brazil

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    Batista Zenón Rodríguez

    1997-12-01

    Full Text Available Através do uso da armadilha Magoam foram capturados 49.010 insetos no período de junho de 1995 a maio de 1996 no município de Pedro Leopoldo MG. Foram identificadas 10 ordens de insetos. Destes, 95,73% pertencem à ordem Díptera. Três famílias, Sepsidae, Syrphidae e Culicidae participaram com 24,65%, 18,53%, e 17,64% respectivamente dos 46.915 dípteros capturados. Dos três locais pesquisados, a mata (local 3 contribuiu com o maior número de insetos capturados correspondentes a 43,99% do total de espécimes colhidos. Os vetares biológicos de D. hominis (com postura aderida importantes por ordem de frequência foram Fannia spp, Musca domestica, Stomoxys calcitrans, Synthesiomyia nudiseta, Ophira sp., sendo esta última notificada pela primeira vez. O gênero Fannia spp. apresentou-se como de maior importância epidemiológica por sua maior frequência e distribuição nos locais de captura.From June 1995 to May 1996, 49,010 insects were captured in the municipality of Pedro Leopoldo, MG, Brazil, using the Magoom's trap. Ten orders were identified and 95.73% of them belong to the order Diptera. Three families, Sepsidae, Syrphidae, and Culicidae, represented 24.65%, 18.53%, and 17.64% respectively, of 46,915 diptera trapped. The bush contributed with the highest number of trapped insects (43,99% when comparsa with stable or pasture. The biological vectors of D. hominis (with adhered, eggs, in order of prevalence, were Fannia spp, Musca domestica, Stomoxys calcitrans, Synthesiomyia nudiseta, Ophira sp. This is the first identification of the genus Ophira sp. as biological vector of D. hominis. The genus Fannia was found to be the most important biological vector of D. hominis in Pedro Leopoldo, MG, Brazil, because of its high frequency and distribution in the three environment conditions searched.

  2. Use of PCR to Detect Mycoplasma hominis and Ureaplasma urealyticum from Semen Samples of Infertile Men who Referred to Royan Institute in 2009

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    Mohammad Hossein Ahmadi

    2010-01-01

    Full Text Available Objective: Infection with genital Mycoplasmas may have harm effects on the reproductivehealth of men, thus leading to male infertility. This study was performed to detectthe prevalence of these bacteria and to study the sperm parameters in infertile men whoreferred to Royan Institute during 2009.Materials and Methods: Semen samples were collected from 220 infertile men and dividedinto three sections. The first section was used for semen analysis, the second sectionfor polymerase chain reaction (PCR in which U4 and U5 primers were used for theamplification of the urease gene of U. urealyticum, and RNAH1 and RNAH2 primers wereused for amplification of the 16S rRNA gene of M. hominis.Results: From a total of 220 semen samples cultured, 15.5% of M. hominis and 40.5% ofU. urealyticum were isolated. Evaluation of semen parameters showed a lower pH in theU. urealyticum positive group and the group which was positive for both bacteria, ratherthan the group which contained no bacteria (p=0.007 and p=0.000, respectively. Also,the mean sperm motility was lower in the group which was positive for both bacteria whencompared with the U. urealyticum positive group (p=0.009.Conclusion: The results of this study show that a high percent of infertile men are infectedwith these bacteria which may lead to pelvic inflammatory disease (PID and infertility,thus isolation of these bacteria in infertile couples with no clinical symptoms is necessaryand can be a part of a sexual transmitted disease (STD control program.

  3. Voz sobre frame relay

    OpenAIRE

    D´Elia, Gabriel Anibal

    2000-01-01

    Esta tesis trata el tema de VOFR, desde la digitalización de la voz hasta su transmisión a través de dicha red, así también como la comparación con otros medios de transporte como VOIP. Dada las características del protocolo frame relay y su disponibilidad se eligió como el medio más apropiado para la transmisión de voz y datos en forma integrada sobre una misma red. El trabajo comienza con una breve explicación de la voz, su digitalización y forma actual de transmisión a través de una red di...

  4. Excertos sobre o sedentarismo

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    Alexandre Palma

    2014-09-01

    Full Text Available O sedentarismo tem sido tratado como algo perfeitamente determinável, objetivo e, portanto, que pode ser apreendido. Por outro lado, o conceito de sedentarismo ainda permanece questionável e, por conseguinte, os instrumentos mostram-se inconsistentes. Neste sentido, o objetivo do presente estudo foi mostrar que diferentes discursos de verdade vêm concorrendo por esse conceito, o que nos motiva a pensar qual é a verdade ou, até mesmo, se há alguma verdade sobre ele. Após apresentarmos diferentes possibilidades de compreender o sedentarismo, através do uso de aforismos, entendemos que postular um discurso como verdade talvez apenas signifique que as pessoas nele creem e que há uma vontade de verdade como uma vontade voltada para o poder.

  5. Sobre la muerte voluntaria

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    Juan Álvarez-Cienfuegos Fidalgo

    2012-03-01

    Full Text Available El problema de cómo morir en nuestra sociedad se ha convertido en una inquietud para los ciudadanos y la reflexión moral. Especialmcnte por dos razones: por el desarrollo de la tecnología médica y por el hecho de que la mayor parte de los fallecimientos tienen lugar fuera del hogar, donde tradicionalmente ocurra el deceso. El presente artículo tiene como objetivo acercarse a esta cuestión. Para ello, se parte de la autonomía del ciudadano, con derecho a decidir sobre lo que considera cuál sea su manera de vivir y de morir. A tal fin, se hace una breve introducción sobre el dolor, el suicidio y la muerte, desde un punto de vista antropológico, pasando, después, a mostrar las cuestiones relacionadas con el problema de la eutanasia, donde la atención se dirigirá, ante todo, al enjuiciamiento crítico del tenso equilibrio en el que se mantienen dos principios fundamentales de la bioética: el de no maleficencia y el de autonomía del ciudadano.The problem of how to die in modern society has become a concern for both the citizen and moral reflection for two reasons in particular: due to developments in medical technology, and because most deaths occur outside the honre, where they traditionaÍlY took place. This paper addresses this issue, starting from citizen autonomy, the right to decide on what s/he considers to be his or her way of living and dying. To this end, a brief introduction considers pain, suicide and death from an anthropological viewpoint. Questions related to the problem of euthanasia are then exposed, with particular attention to, above all the critical judgement of the tense balance in which two fundamental principies of bioethics are held, the principle of non-maleficence and that of citizen autonomy.

  6. Sobre el nombre "Quechua"

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    Rodolfo Cerrón-Palomino

    1985-06-01

    Full Text Available Uno de los primeros problemas con el que a menudo tropiezael estudioso de los idiomas ágrafos, como es el caso de las lenguasaborígenes de América, es el de su denominación (glotónimo.En la medida ,en que, por razones históricas, las noticias sobretales lenguas, y por consiguiente los primeros estudios sobre lasmismas, surgen a partir del contacto con los miembros de la civilización occidental, los nombres de los distintos idiomas, así comode los grupos étnicos que los manejan, son el resultado casi siempre arbitrario cie designaciones fortuitas hechas por viajeros, misiioneros y exploradores, quienes por lo general se basan, para ello, bien en descripciones físicas de los grupo étnicos, en ciertos indios geográficos que constituyen el habitat de los mismos, e incluso en prejuicios que se nutren de un marcado europocentrismo.

  7. Refletindo sobre idosos institucionalizado

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    Cenir Gonçalves Tier

    2004-06-01

    Full Text Available Trata-se de uma pesquisa qualitativa, descritiva que teve por objetivo identificar como vem sendo o relacionamento idoso-família, identificando os motivos que levaram os familiares a optarem pela institucionalização do seu idoso, obtendo-se também a visão da família sobre o cuidado no lar. Para coleta dos dados utilizou-se um instrumento com perguntas abertas. A coleta dos dados efetivou-se nos meses de agosto de 2002 a julho de 2003, sendo sujeitos da pesquisa, familiares de idosos de uma instituição asilar em um município da região noroeste do Rio Grande do Sul. Através da análise de conteúdo constatou-se que a maior dificuldade encontrada pela família, visando dispensar cuidados ao idoso é a "falta de tempo para cuidar" do seu familiar idoso, pois a maioria deles exige cuidados em tempo integral.

  8. Gregory of Nyssa. De hominis opificio. O obrazě člověka. The Fourteenth-Century Slavonic Translation. A Critical Edition with Greek Parallel and Commentary

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    Sels, Lara

    2009-01-01

    Gregory of Nyssa’s De hominis opificio is a key text for the understanding of Eastern Christian anthropology. In the fourteenth century a Serb translated the 31 chapters of this opus from the Greek. The present volume contains a critical edition of the Slavonic text together with the Greek original and an extensive commentary in which text-critical, linguistic and translation-related issues are examined.

  9. Diferenciação morfológica das gônodas em larvas de Dermatobia Hominis (Diptera: Cuterebridae

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    Edy de Lello

    1984-06-01

    Full Text Available O trabalho descreve o desenvolvimento morfológico das gônadas, durante os três períodos larvais da Dermatobia hominis. Em larvas do 1° e 2° instar com a metodologia empregada, de dissecção sob lupa, foi impossível individualizá-las, mas elas aparecem nos cortes totais dessas larvas, como um pequeno aglomerado celular envolto por uma túnica acelular, medindo ao redor de 30 µm de diâmetro nas primeiras, e 54 µm nas segundas. Microscopicamente, apresentam células com dois tipos de núcleos, uns grandes arredondados e frouxos e outros menores, e ovóides; nas larvas mais jovens ambos os tipos nuclearesse misturam enquanto que nas mais velhas os maiores permanecem no interior e os menores se ajeitam ao redor da gônoda e entre os maiores. Anatomicamente a distinção entre testículo e ovário ocorre em larvas do 3° instar com peso a partir de 400mg.Morphological development of the gonads during the three larval instars of Dermatobia hominis is described. It was impossible to individualize the gonads on the first (L1 and second (L2 insars, by dissection under stereoscopic microscope. They appear in whole sections, as a small cellular cluster surrounded by a tunica, not exceeding 30 µm in diameter in L1, and 60 µm in L2. Histologically they show two types of cells, one with large round nuclei and other with small ovoid ones; these latter are located under the tunica and among the former. In the third instar the larvae grow considerably and gain weight from 50 to 900mg. In larva weighting between 100 to 200mg, under the stereoscope the gonads appear as a small dot; in larvae weighting between 250 to 350mg they appear as two transparent spheres of approximately 100 µm of diameter. The anatomical distinction between testicle and ovary ins only possible in larvae weighting 400 mg or more. This anatomical distinction is followed by rearrangements of the different types of cells.

  10. Investigation on Symbiosis of Mycoplasma hominis in Trichomonas vaginalis in Some Areas of Guizhou Province%贵州省部分地区阴道毛滴虫与人型支原体共生情况

    Institute of Scientific and Technical Information of China (English)

    匡贵榕; 衣凤芸; 杨宇箭; 程其会; 王丽娟; 吴家红

    2015-01-01

    目的:了解贵州地区阴道毛滴虫临床分离株与人型支原体共生情况。方法:以贵州部分地区女性阴道毛滴虫病患者阴道后穹隆分泌物中采集的阴道毛滴虫虫株,实验室达到纯培养后,采用Chelex-100的方法提取滴虫基因组DNA,设计人型支原体16S rDNA特异引物,进行聚合酶链式反应( PCR),琼脂糖凝胶电泳法对PCR产物进行分析,观察滴虫细胞内人型支原体共生情况。结果:从临床共采集到165株虫株中,83株获得纯培养;83株中人型支原体检测结果显示有47株阳性,阳性率为56.6%。结论:阴道毛滴虫虫株与人型支原体共生情况在我国贵州地区普遍性存在。%Objective:To investigate the symbiosis of Mycoplasma hominis in Trichomonas vaginalis in Guizhou Province. Methods:The clinical strains of T. vaginalis were collected from the hospitals and family planning service stations in Guiyang city and Anshun city. After pure culture in lab,the ge-nome DNA of T. vaginalis was extracted by Chelex-100,and 16S rDNA fragment of M. hominis was amplified by PCR with the specific primers. Agarose gel electrophoresis analysis was conducted and the symbiosis of M. hominis in T. vaginalis was observed. Results:A total of 165 strains were collected from the hospitals and family planning service stations,and 83 clinical strains attained pure culture,of which 47 strains were detected positively for M. hominis. The positive rate was 56. 6%. Conclusions:The symbiosis of M. hominis in T. vaginalis is prevalent in Guizhou province.

  11. Relationship of Mycoplasma Hominis and Bacterial Vaginosis%人型支原体与细菌性阴道病相关性的研究

    Institute of Scientific and Technical Information of China (English)

    叶千红; 尚红; 陈昕; 侯香圃; 张淑兰

    2001-01-01

    目的:初步探讨人型支原体(mycoplasm hominis,MH)与细菌性阴道病(bacterial vaginosis,BV)的相关性,探索我国BV的致病因子.方法:根据BV的诊断标准选择100名BV者,同时选择177名健康体检者做正常对照组,取阴道分泌物进行MH培养.检测结果经SAS 6.11统计软件包处理,计算χ2值,判断MH与BV的相关性.结果:BV者MH阳性检出率为43%,明显高于对照组(5.6%),统计学差异十分显著(P<0.001).结论:MH与BV有明显的相关性,可能是BV的致病因子之一.

  12. Acueducto pretensado sobre el Holme

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    Editorial, Equipo

    1958-04-01

    Full Text Available Breve descripción del paso de una canalización de 0,60 m de diámetro, destinada al abastecimiento de aguas, sobre el río Holme (Inglaterra. El puente formado tiene dos vigas de hormigón pretensado, cuyas armaduras se componen de barras McCall.

  13. 阴道毛滴虫与人型支原体共生对铁氧还蛋白基因影响%Effects of the Symbiosis of Trichomonas Vaginalis with Mycoplasma Hominis on Ferredoxin Gene

    Institute of Scientific and Technical Information of China (English)

    刘晓东; 温雯静; 薛长贵

    2011-01-01

    We isolated 30 Trichomonas vaginalis for the PCR detection from the gynecological outpatients in the Affiliated Hospital of Zhengzhou University using the specific 16s rDNA primers of Mycoplasma hominis. The results showed that there were 25 cases of Mycoplasma hominis infection, with the infection rate of 83. 33%. This gave a clew that the symbiosis of Trichomonas vaginalis with Mycoplasma hominis may be of certain generality in China. We sequenced the ferredoxin gene of 10 Trichomonas vaginalis where 5 Mycoplasma hominis were positive and five negative, and found that the ferredoxin (Fd) gene of the 10 Trichomonas vaginalis were exactly the same. But compared to the genes in the GenBank. A comparative analysis of the gene revealed that there were 3 more ctg bases at the 200th position of encoding leucine, but this did not lead to changes in reading frame. The gene homology was 99%.%采用人型支原体16s rDNA的特异引物,对从郑州大学附属医院妇科门诊患者分离到的30株阴道毛滴虫进行PCR检测,结果有25株感染人型支原体,感染率为83.33%,这显示了阴道毛滴虫和人型支原体之间的共生关系在中国具有普遍性.并对10株阴道毛滴虫(5株人型支原体阳性和5株人型支原体阴性)的铁氧还蛋白(Fd)基因进行测序,探讨人型支原体对Fd基因的影响,结果发现10株阴道毛滴虫的Fd基因完全相同,但与GenBank中的基因进行比较分析,在第200位多出ctg三个碱基,编码亮氨酸,但并未导致读码框架改变.基因同源性为99%.

  14. 人型支原体实时荧光聚合酶链反应检测方法的初步建立%Establishment of real-time PCR assay to detect Mycoplasma hominis

    Institute of Scientific and Technical Information of China (English)

    张慧芳; 张建中; 赵飞

    2012-01-01

    目的 建立一种快速、灵敏和特异的人型支原体实时荧光聚合酶链反应(real-time PCR)检测技术.方法 依据人型支原体gap基因保守区域使用Beacon Designer 7.0软件设计引物和探针,建立人型支原体real-time PCR检测方法并优化.对优化后的方法进行实验室灵敏度、特异度和检测限评价,并与聚合酶链反应(PCR)进行比较.结果 该real-time PCR对于人型支原体的检测限为50 cfu,PCR检测限为5×103cfu,其检测灵敏度为PCR的100倍.所建立的检测方法对其他10种常见支原体、12种泌尿生殖道感染病原菌染色体及人类染色体的扩增均为阴性.结论 本研究建立的real-time PCR方法可灵敏、特异的检测人型支原体,有望用于临床标本检测.%Objective To develop a real-time PCR assay to detect Mycoplasma hominis. Methods By analyzing the gap gene of M. hominis, an optimized real-time PCR assay was designed. The specificity, sensitivity and detection limit of the assay were evaluated and compared with conventional PCR assay by using standard concentration DNA of M. hominis. Results The detection limit of the assay was about 50 cfu and the specificity of the assay appeared to be 100%. The sensitivity of this real-time PCR assay was 100 times higher than that of conventional PCR. Conclusion This real-time PCR assay might be a suitable method for the clinical detection of M. hominis.

  15. Hemilucilia segmentaria (Fabricius, 1805 (Diptera: Calliphoridae as new biological vector of eggs of Dermatobia hominis (Linnaeus Jr., 1781 (Diptera: Oestridae in Reserva Biológica do Tinguá, Rio de Janeiro, Brazil

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    Marinho Clarissa Rezende

    2003-01-01

    Full Text Available The aim of this note was to record for the first time the finding of Hemilucilia segmentaria acting as biological vector of Dermatobia hominis, during a study of the diversity of Calliphoridae at Reserva Biológica do Tinguá, Rio de Janeiro, Brazil. The insects were captured using traps baited with chicken vicera, for a period of 28-30 h twice per month. In the period of one year, 1987 insects were collected, 7.5% of which belonged to the H. segmentaria; of these a female was captured in May 2001, carrying a mass of 20 eggs on the left side of its abdomen.

  16. Prevalence of Chlamydia trachomatis, Ureaplasma spp., Mycoplasma genitalium and Mycoplasma hominis among outpatients in central Greece: absence of tetracycline resistance gene tet(M over a 4-year period study

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    A. Ikonomidis

    2016-01-01

    Full Text Available A total of 301 men and women attending local urologists and gynaecologists in the state of Thessaly, central Greece, were tested for Chlamydia trachomatis, Ureaplasma spp., Mycoplasma genitalium and Mycoplasma hominis DNA. Investigation of the tet(M gene, which confers tetracycline resistance in these genera, was also performed. Low incidence of C. trachomatis and Mycoplasma spp. as well as high prevalence of Ureaplasma spp., especially among women, were found. The tet(M gene was absent in all cases, notably in a region where doxycycline administration remains the first therapeutic option unless special medical conditions direct otherwise.

  17. 人型支原体共生对甲硝唑临床治疗阴道毛滴虫感染的影响%Mycoplasma hominis symbiosis and Trichomonas vaginalis metronidazole clinical treatment efficacy

    Institute of Scientific and Technical Information of China (English)

    谢成彬; 王频佳; 吴雨露; 苏喆

    2013-01-01

    目的:研究人型支原体共生对甲硝唑治疗阴道毛滴虫感染患者临床效果的影响.方法:分离培养阴道毛滴虫并检测其甲硝唑最小致死浓度(MLC).PCR检测滴虫细胞内人型支原体DNA,比较共生阴道毛滴虫株在人型支原体清除前后甲硝唑MLC的变化.将患者分为阴道毛滴虫单独感染组(滴虫组)与人型支原体共生感染组(共生组),比较甲硝唑对滴虫组与共生组患者进行标准治疗后的临床治疗效果.结果:(1) 200株阴道毛滴虫的甲硝唑MLC为0.2~ 200μg/ml,其中132株(66.0%)低MLC虫株(≤6.3μg/ml)、45株(22.5%)中MLC虫株(12.5 ~ 25 μg/ml)、23株(11.5%)高MLC虫株(50~ 200μg/ml);(2)76株(38.0%)阴道毛滴虫检出人型支原体共生,其中低、中、高MLC虫株共生率分别为12.9% (17/132)、84.4%(38/45)和91.3% (21/23),差异有统计学意义(P<0.01);(3)200例患者甲硝唑治疗的总有效率为82.0%,其中滴虫组与共生组总有效率分别为95.2%和60.5%,差异有统计学意义(P<0.01);低、中、高MLC共生组总有效率分别为76.5%、65.8%和38.1%,差异有统计学意义(P<0.05);(4)11株共生虫株经多西环素作用清除人型支原体后,其甲硝唑MLC基本无变化.结论:人型支原体共生可能与甲硝唑治疗阴道毛滴虫的临床效果有关,但未发现直接相关关系.%Objective:To investigate the effect of Mycoplasma hominis symbiosis on Trichomonas vaginalis metronidazole clinical treatment efficacy.Methods:T.vaginalis isolates were collected and purified from clinical specimen and the metronidazole minimum lethal concentration (MLC) of these isolates was determined,M.hominis DNA in T.vaginalis was detected by polymerase chain reaction (PCR).After clearance of M.hominis in T.vaginalis by doxycycline,MLC was determined and compared with that before clearance.These patients were categorized as T.vaginalis alone infection cases or Mycoplasma hominis symbiosis cases

  18. Breve informe sobre la coca

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    Lauro Hinostroza García

    1985-12-01

    Full Text Available El presente informe se integra en la investigación dirigida por el doctor Juan Ossio, por encargo de la Organización de los Estados Americanos (O.E.A. sobre “EFECTOS SOCIALES, CULTURALES, MÉDICOS Y ECONÓMICOS DEL USO TRADICIONAL DE LA COCA EN POBLACIONES DEL ANTIPLANO Y SIERRA DEL PERÚ, EN ESPECIAL DE LAS POBLACIONES INDÍGENAS”. Expone tópicos sobre la hoja de coca en la cultura andina: criterios clasificatorios, mitos, canciones y una muestra de qatipay o seguimiento. Este último es una práctica ritual importante para el campesinado andino, orienta el presente, pasado y futuro de toda su actividad. Cada hoja, por su forma, posición y tamaño, develará los roles sociales que ha de asumir, es decir los misterios del tiempo.

  19. Sobre la verdad en periodismo

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    Julio Estremadoyro Alegre

    2011-05-01

    Full Text Available Cuando un periodista informa sobre un desastre natural, con granpérdida de vidas, las autoridades y las fuentes de primera manodan cifras que son difundidas como verdades del suceso. Sinembargo, y la experiencia lo confirma, al día siguiente la cifraspueden haber cambiado y, por lo tanto, la verdad informativa esotra. Y así, sucesivamente, cada día habrá novedades y nuevas cifras verdaderas.

  20. Séquençage du génome du parasite intestinal Blastocystis sp. (ST7) : vers une meilleure compréhension des capacités métaboliques d'organites apparentés aux mitochondries chez ce microorganisme anaérobie

    OpenAIRE

    Roussel, Michaël

    2011-01-01

    Blastocystis sp. is a highly prevalent anaerobic eukaryotic stramenopile parasite found in the intestinal tract of humans and various animals. This microorganism, sometimes associated with acute intestinal disorders, could be responsible for functional intestinal disorders such as the irritable bowel syndrom (IBS). As part of a collaborative sequencing project with the Genoscope (CEA Evry, France), we were able to caracterize the smallest stramenopile genome sequenced to date (18.8 Mbp) with ...

  1. Mycoplasma hominis Symbiosis and Trichomonas vaginalis Metronidazole Resistance%人型支原体共生与阴道毛滴虫甲硝唑耐药性的关系

    Institute of Scientific and Technical Information of China (English)

    王频佳; 谢成彬

    2012-01-01

    目的 研究人型支原体(Mycoplasma hominis)的共生与阴道毛滴虫(Trichomonas vaginalis)甲硝唑耐药性的关系.方法 2010年11月至2011年7月,自四川省妇幼保健院妇科门诊患者生殖道分泌物中分离出160株阴道毛滴虫,用梯度浓度1 024、512、256……4、2和1 μg/ml甲硝唑分别处理该批虫株,以死亡率≥90%的最低浓度作为甲硝唑最小致死浓度(MLC).以160个阴道毛滴虫分离株中提取的DNA为模板,用PCR技术特异性扩增人型支原体16S rRNA基因,检测滴虫细胞内是否有人型支原体共生.对检出人型支原体DNA的分离株用32μg/ml多西环素清除支原体,比较清除前后甲硝唑MLC的变化.结果 160个阴道毛滴虫分离株中甲硝唑MLC为1~8μg/ml的占61.3%(98/160),16~32μg/ml的占26.3%(42/160),64~256μg/ml的占12.5%(20/160).PCR检测结果显示,有61株(38.1%)检出人型支原体DNA,其中MLC为1~8μg/ml的分离株检出率为13.3%(13/98),16~32μg/ml的分离株检出率为73.8%( 31/42),64~256μg/ml的分离株检出率为85.0%(17/20),不同MLC范围的分离株人型支原体检出率差异有统计学意义(P<0.01).用多西环素处理后,61株中仅有8株支原体被清除,清除前后甲硝唑MLC无明显变化.结论 四川地区的阴道毛滴虫分离株对甲硝唑表现出一定程度的耐药性,人型支原体的共生可能与之有关,但尚未发现直接证据.%Objective To investigate the relation of Mycoplasma hominis symbiosis and the resistence of Trichomonas vaginalit to metronidazole. Methods From November 2010 to July 2011, 160 isolates of T. Vaginalis were collected from the genital tract secretion of gynecological out-patients at the Sichuan Provincial Hospital for Women and Children. The minimum lethal concentration (MLC) to metronidazole of these isolates was determined by an in. Vitro sensitivity assay with different concentration gradients of metronidazole (from 1 to 1 024 μg/ml), and M

  2. Nota sobre Keynes y Brentano

    OpenAIRE

    Crespo, Ricardo F.; IAE (Universidad Austral) y CONICET

    2013-01-01

    Este trabajo explora la posibilidad de una relación entre el pensamiento de John Maynard Keynes y el del filósofo alemán Franz Brentano (1839-1917) en los campos lógico y epistemológico. George E. Moore -profesor de Keynes- y Keynes mismo leyeron y alabaron el libro de Brentano El origen del conocimiento moral (1889). ¿Tomó Keynes algunas ideas lógicas o epistemológicas de Brentano? El trabajo sugiere que, a pesar de ciertas similitudes entre las ideas lógicas y sobre la verdad de Keynes y Br...

  3. Thomas Hobbes: sobre el miedo

    OpenAIRE

    Carlos Bührle

    2004-01-01

    Este trabajo pretende echar cierta luz sobre el papel del miedo en el pensamiento político de Hobbes. Sabido es que esta pasión subyace al contrato social, en el sentido de que impulsa al hombre a abandonar el estado de naturaleza a fin de encontrar seguridad y de deshacerse de la amenaza de la muerte violenta. Teniendo en cuenta que el miedo y la muerte son inseparables en la filosofía de Hobbes, surge una pregunta fundamental: ¿hasta qué punto podemos decir que el miedo desaparece completam...

  4. Modelos ambientales sobre la vejez

    OpenAIRE

    Izal, María; Fernández-Ballesteros, Rocío

    1990-01-01

    La perspectiva ambiental en el estudio de la vejez es probablemente un ámbito poco conocido. En este trabajo se expone una revisión crítica de los principales modelos de explicación de las relaciones ambiente-conducta en la vejez. Finalmente, se discute sobre la superación de algunas limitaciones presentes en estas formulaciones teóricas, presentándose posibles alternativas teóricas y metodológicas para el desarrollo de esta área.

  5. Historicismo: notas sobre um paradigma

    OpenAIRE

    José D’Assunção Barros

    2012-01-01

    Este artigo tem por objetivo desenvolver algumas considerações sobre o Historicismo e sua possibilidade de ser examinado como um dos paradigmas que concorrem para o estabelecimento de uma história científica no século XIX. O primeiro ponto tratado é relativo às origens do paradigma Historicista na transição do século XVIII para o século XIX. Na sequência, aproximamo-nos da apropriação conservadora do paradigma historicista no novo contexto no qual a burguesia européia está assentada no Poder,...

  6. Entorno colaborativo sobre laboratorios remotos

    OpenAIRE

    Grosclaude, Eduardo; Sznek, Jorge Eduardo; Ramos Garcia, Vicente; Bertogna, Mario Leandro; López Luro, Francisco; Zanellato, Claudio

    2006-01-01

    El proyecto de investigación Software para Procesos Colaborativos incluye una línea de acción sobre el desarrollo de experiencias en Laboratorios Remotos. Esta línea está dedicada a complementar las tareas de aprendizaje con un soporte tecnológico de sistemas, entendido como todas aquellas actividades de infraestructura que promuevan la disponibilidad de recursos y apoyen la escalabilidad de procesos en la tarea educativa apuntalada por tecnologías de información. En este trabajo se describe ...

  7. Laboratory Model for Cryptosporidium hominis IbA19G2 Subtype in Mongolian Gerbils%人隐孢子虫IbA19G2基因亚型蒙古沙鼠感染模型的建立

    Institute of Scientific and Technical Information of China (English)

    王强; 杨楠; 菅复春; 王荣军; 宁长申; 张龙现

    2011-01-01

    人隐孢子虫Cryptosporidium hominis是人类的主要感染虫株,目前已获取且传代保存的国内分离株较少,在生物学特性研究也未见报道.在本试验自感染前第10天起至排卵囊结束后14天,采用胃管灌服地塞米松(0.75 mg/(只·2天))抑制蒙古沙鼠免疫力,对国内C.hominis Ib亚型分离株生物学特性进行研究.结果显示,感染后第3天,感染组有卵囊排出,感染后第6天出现高峰期,此后逐渐下降直至感染后第10或11天排卵囊结束.感染前后基于18S rRNA和gp60基因分析,表明遗传学无差异.建立了持续稳定的C.hominis Ib亚型分离株啮齿动物模型,为C.hominis卵囊扩增和进一步研究其生物学特性等提供了重要的参考依据.%Researches have sbown that Cryptosporidium hominis is one of the main Cryptosporidium parasites for human cryptosporidiosis in China. However, only a few isolates of C. hominis were obtained and aerially sub-cultivated in laboratory up to date, so the biological characteristics of C. hominis remaina unreported in our country. In the present work, Mongolian gerbils (Meiiones unguiculataus) were orally inoculated with 0. 75 mg of dexamethasone/animal by using gastric tube drench every two days from the 1Oth day before oocyst ingestion. The results showed that the gerbils started to shed oocysts in the third day after inoculation ( DAI) , the number of shed oocysts increased obviously, reached the peak value at the 6th DAI,and then dropped graduaUy until lOth or llth DAI. The 18S rRNA and gp60 gene analysis performed before and after inoculation revealed no genetic difference of C. hominis oocysts. A stable rodent model of C. homiais Ib subtype infection has been successfully established, which may be an important value for the further study on the biological characteriatica of C. hominis.

  8. Recursos y enlaces sobre comunidades de aprendizaje

    OpenAIRE

    Ferrer Esteban, Gerard

    2005-01-01

    Obras y artículos sobre el proyecto Comunidades de Aprendizaje desarrollado en el Estado español. Tesis doctorales sobre comunidades de aprendizaje. Fundamentación teórica de las comunidades de aprendizaje. Sitios en Internet relacionados con las comunidades de aprendizaje y proyectos afines de desarrollo comunitario en contextos escolares.

  9. On GAMER, plataforma de continguts sobre videojocs

    OpenAIRE

    Villegas Bonachera, José Ricardo

    2015-01-01

    Desenvolupament d'una plana web amb disseny responsiu sobre videojocs en català a partir del gestor de continguts WordPress. Desarrollo de una página web con diseño responsivo sobre videojuegos en catalán a partir del gestor de contenidos WordPress. Bachelor thesis for the Multimedia program on Computer Science.

  10. Escenarios sobre el orden internacional

    Directory of Open Access Journals (Sweden)

    Luis Dallanegra Pedraza

    2012-01-01

    Full Text Available Luego de las grandes cambios que se dieron con la caída del Muro de Berlín y la desintegración en la URSS, se analizan, desde una perspectiva realista-sistémicaestructural, los componentes que intervienen en la generación del orden mundial, qué factores intervienen y cuáles son las diferentes aspiraciones respecto de las características que debería tener. Se tiene en especial consideración la creciente heterogeneización del sistema y la manera en que actores transnacionales operan como motores del futuro orden, predominando por sobre las posibilidades del actor históricamente dominante: el Estado-Nación. Se estudian las características principales del funcionamiento del sistema internacional y el significado de su marco institucional.

  11. Constitucional sobre la justicia ordinaria

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    José Luis Cea Egaña

    2005-01-01

    Full Text Available El artículo analiza el efecto vinculante y de irradiación de las sentencias del Tribunal Constitucional sobre el conjunto del ordenamiento jurídico y de sus órganos. El autor toma partido por la consideración de la ratio decidendi del fallo como vinculante junto al dictum de la sentencia respectiva. Se plantea la necesidad que la LOC del Tribunal Constitucional explicite los efectos vinculantes de los fallos respecto de los tribunales ordinarios y especiales. Finalmente analiza los efectos de la res iudicata en el ámbito del control reparador de constitucionalidad de preceptos legales en control concreto y en control abstracto.

  12. Historicismo: notas sobre um paradigma

    Directory of Open Access Journals (Sweden)

    José D’Assunção Barros

    2012-08-01

    Full Text Available Este artigo tem por objetivo desenvolver algumas considerações sobre o Historicismo e sua possibilidade de ser examinado como um dos paradigmas que concorrem para o estabelecimento de uma história científica no século XIX. O primeiro ponto tratado é relativo às origens do paradigma Historicista na transição do século XVIII para o século XIX. Na sequência, aproximamo-nos da apropriação conservadora do paradigma historicista no novo contexto no qual a burguesia européia está assentada no Poder, combinando-se com a consolidação dos modernos estados-nações. A especificação das características deste paradigma constituem o principal ponto de análise

  13. As monografias sobre plantas medicinais

    Directory of Open Access Journals (Sweden)

    Valdir F. Veiga Junior

    2008-09-01

    Full Text Available As monografias sobre plantas medicinais ou drogas vegetais contêm informações para atender não somente aos órgãos de regulamentação, mas também às empresas industriais farmacêuticas e farmácias, e mesmo ao público consumidor. Este artigo revê as monografias da Comissão E, do American Botanical Council, ESCOP, PDR e da Organização Mundial de Saúde em suas origens, objetivos e formatos. Duas publicações recentemente organizadas pela FIOCRUZ do Rio de Janeiro e as contribuições da Farmacopéia Brasileira também são avaliadas.

  14. Puente sobre el rio Medway

    Directory of Open Access Journals (Sweden)

    Freeman, Fox, Ingenieros

    1963-02-01

    have been prestressed both longitudinally and transversally. Between the two circulation roadways an elastic barrier has been placed, supported on metal posts.El puente sobre el río Medway tiene la misión de salvar dicho río en el nuevo trazado de carretera necesario para aliviar el denso tráfico estival entre Dower y Londres. Está situado en las proximidades de la ciudad de Rochester. La estructura se ha construido utilizando las vías de concurso. De todos los tipos presentados se eligió una estructura de hormigón armado. La longitud total de la obra es de 1.000 m. Los tres tramos principales de la obra salvan el río. El central es de 152 m de luz, y los dos adyacentes son de 103 m cada uno. Los accesos tienen un total de 18 tramos, que arrojan una longitud de 654 m. La anchura del puente es de 34 m, y la calzada se ha subdividido en dos direcciones de tráfico de 7,30 m cada una y, además, se han dejado dos espaldones laterales de 2,40 m de anchura. En planta se puede admitir que el puente tiene una alineación recta con una ligerísima curva, y que en el plano vertical presenta una pendiente de desagüe entre las pilas 9 y 10. Los estribos son de hormigón armado: tipo celular el del este y de hormigón en masa el del oeste. Los estribos se apoyan sobre pilotes hexagonales de hormigón armado cuya circunferencia circunscrita tiene 55 cm de radio y de 12 a 20 m de longitud. Cuando el firme se hallaba a profundidades inferiores a 6 m, los estribos se cimentaban directamente sobre el firme. Las pilas constituyen pórtico y tienen una altura variable de 9 a 30 m sobre el nivel del suelo. Los tramos de acceso forman una estructura continua simplemente apoyada. Cada tramo se compone de ocho vigas longitudinales de 30 a 41 m de longitud y de 190 toneladas cada una. Estas vigas son del tipo pretensado y prefabricado. El tramo central es del tipo cantilever, constituido por dos brazos, de 30 m de longitud, formados por vigas cajón. Cada una de estas vigas consta

  15. Puente sobre el Oosterschelde, Holanda

    Directory of Open Access Journals (Sweden)

    Hoving, H. T.

    1967-03-01

    Full Text Available This new bridge, forming part of the Delta project, is made of concrete, and was built by successive overhangs. Precast units were mainly used. The bridge is 5,000 m long, and is 11.85 m wide. The main spans are 95 m in length, except one, which can be opened up to sea traffic, which is 40 m long. The free height of the bridge over the sea is 15 m, which is insufficient for large ships. This required that one of the spans, in deep water, should open up, on hinges, and provide free passage to large ships. The intermediate bridge supports are of special interest. They rest on piles which go down to 38 m. They are hollow, cylindrical, of 4.35 m external diameter, and are driven into the ground in several stages, as they penetrate through the sands at the bottom of the Oosterschelde. To drive them in, an excavating device, inside the pile, was utilised, and also a dredging and suction mechanism, to clean out the bottom, and allow the piles to sink by gravity. But when external friction did not allow this to happen, the piles were weighed down with up to 500 tons of ballast. A series of three of these piles, in a row, makes up each bridge support. A cross member over the pile heads supports, in turn, the bridge deck. The bridge was built in successive sections, overhanging from each support, and then finally connected with a central portion. Use of precast units has been very extensive, so that the bridge can be justiaflably regarded as being prefabricated.Este nuevo puente, que forma parte del Plan de obras denominadas del Delta, es de hormigón y se ha construido por el procedimiento de voladizos sucesivos, utilizando, en su mayor parte, elementos prefabricados. Tiene una longitud total de 5.000 m; una anchura de 11,85 m; tramos principales de 95 m de luz, salvo uno, levadizo, que tiene 40 m. La altura libre sobre las ag^uas del mar es de 15 m, que es insuficiente para las naves de gran tonelaje; esto exigió que uno de sus tramos, en aguas

  16. Somos un envase : Discursos sobre el aborto

    OpenAIRE

    Löcher, Jennifer Irene

    2014-01-01

    No hay persona que no tenga opinión acerca del asunto y, cada vez más, la sociedad argentina debate sobre la pregunta de si se debe legalizar/despenalizar el aborto o no. Nunca antes se habló tanto sobre el tema públicamente: se publican artículos en los diarios, hay manifestaciones a favor y hay manifestaciones en contra de la legalización del aborto. El siguiente trabajo pretende relevar y analizar discursos que circulan socialmente alrededor del debate sobre el aborto. (Párrafo extraíd...

  17. Concepciones sobre la docencia universitaria de calidad

    OpenAIRE

    Arbeláez López, Ruby; Fortes del Valle, M. Carmen; Grau Rubio, Claudia

    2008-01-01

    Con esta investigación hemos querido participar en el proceso de búsqueda y construcción del sentido de calidad aplicado a la docencia universitaria, con el objetivo general de evidenciar las concepciones sobre docencia de calidad que pueden deducirse de los procesos de evaluación de los profesores universitarios. Nuestra meta final intenta propiciar la revisión de las políticas educativas y el enriquecimiento de la cultura sobre docencia y sobre evaluación docente. La mayor aportación de nue...

  18. Sobre a psicopatologia dos atos

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    Roberto Calazans

    2015-07-01

    Full Text Available Este artigo pretende apresentar a psicopatologia dos atos a partir do esquema de Jacques Lacan sobre a angústia. Diferenciamos os acting-out, a passagem ao ato, os sintomas, as inibições e a angústia fazendo uma duplicação do esquema de Lacan levando em consideração os registros do simbólico, do real e do imaginário. Esse esquema permite uma abordagem da questão dos atos do sujeito sem cair em uma perplexidade do clínico, nem na confusão entre os atos e a ação. Permite também uma melhor orientação do clínico em relação às diversas modalidades de respostas subjetivas que não se restringem à questão sintomática, que é o campo inaugural da clínica psicanalítica desde a sua fundação por Sigmund Freud. Acreditamos contribuir, assim, para um maior esclarecimento desse esquema no ensino de Lacan e para que ele possa servir de orientação para o tratamento dos atos do sujeito na clínica.

  19. Estudo multicasos sobre atividades inovativas

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    Sonia Regina Hierro Parolin

    2013-09-01

    Full Text Available Em estudos sobre competitividade argumenta-se que as empresas necessitam de estratégias baseadas em inovações e de capacidades internas em constantes e intensas transformações, não somente nos padrões tecnológicos, mas também no encadeamento do processo de gestão das atividades inovativas, como processos dinâmicos, não lineares e diversificados, em função de múltiplos fatores dos ambientes interno e externo. Para contribuir com essas discussões, neste artigo apresenta-se um estudo multicasos em quatro indústrias de médio e grande portes, de segmentos, densidades tecnológicas e históricos com inovações diferentes entre si. Como principais resultados, salientam-se o encadeamento das atividades inovativas como parte de uma estratégia organizacional para obter resultados com inovação e a afluência de todas as pessoas, e não somente as alocadas em pesquisa e desenvolvimento, para o cumprimento dessa estratégia.

  20. PERSPECTIVAS INFANTILES SOBRE LA TERRITORIALIDAD

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    Noelia Enriz

    2009-12-01

    Full Text Available En este trabajo nos proponemos un acercamiento a las diversas ideas infantiles sobre el territorio habitado, en el contexto particular de la población mbyá guaraní de Misiones. La fragilidad territorialidad a la que es expuesta la población indígena en Argentina se manifiestan cotidianamente en diversas áreas. La población mbyá, ha sido surcada por los límites de los estados nacionales de Paraguay, Brasil y Argentina. La sedentarización y los cercamientos de tierras en los diferentes estados, han impulsado transformaciones de las lógicas de subsistencia, de habitación y organización comunitaria. En el caso de la provincia de Misiones la acreditación burocrática de los títulos de tierras se encuentra aplazada, el estado no se compromete con la concreción de este derecho.

  1. Puente colgante sobre el Lillebaelt

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    Ostenfeld, Chr.

    1971-09-01

    Full Text Available This bridge, which was opened to traffic in October 1970 over the Lillebaelt, is the first of its kind in Denmark. It consists of a central span of 600 m, and two lateral ones of 240 m length each. The suspension cables, each 1,500 m, are the world's largest prefabricated cables. The bridge has original features, such as flaps on the main box girder, and new design of the anchorage blocks. These and other details are described in the article.Inaugurado en octubre de 1970, el puente colgante sobre el Lillebselt es el primer puente danés de este tipo. Consta de un tramo central de 600 m y dos tramos laterales de 240 m. Los cables sustentantes son, con sus 1.500 m de longitud, los mayores cables prefabricados del mundo. El puente presenta características origínales —empleo de «flaps» en la vigacajón de la superestructura, concepción nueva de los macizos de anclaje...— que, junto con el sistema de ejecución y las demás particularidades de la obra, son descritas en el artículo.

  2. Los derechos de autor sobre obtenciones vegetales

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    Rosario Silva Gilli

    2015-01-01

    Full Text Available Contenido: Las creaciones en la agricultura. Los derechos de autor sobre obtenciones vegetales. El Convenio Internacional para la protección de las obtenciones vegetales (París 1961 y sus revisiones de Ginebra 1972, 1978,1991 Objeto de la protección legal. Condiciones del cultivar. Alcance de la protección. La protección de obtenciones vegetales en el Mercosur. Los Derechos de autor en el Mercosur. El Protocolo de Armonización de normas sobre Propiedad Intelectual en el Mercosur. El Acuerdo de Cooperación y facilitación sobre la protección de las obtenciones vegetales en los Estados Partes del Mercosur.  Alcance de la normativa Mercosur. Los Derechos sobre cultivares en Uruguay. Los Derechos de autor como derechos humanos. ¿Derechos de autor vs. derecho de propiedad intelectual? Reflexión final

  3. PERSPECTIVAS INFANTILES SOBRE LA TERRITORIALIDAD

    Directory of Open Access Journals (Sweden)

    NOELIA ENRIZ

    2009-12-01

    Full Text Available In this paper we propose a different approach to children's ideas about the geographical area in the particular context of the Mbyá Guarani population, from Misiones (Argentine. The territorial fragility, that is exposed to the indigenous people in Argentina aremanifested daily in several areas. Mbyá population, has been crossed by the boundaries of national states of Paraguay, Brazil and Argentina. The sedentary and enclosures land processes in different states, have prompted changes in the logic of subsistence, housing and community organization. In Misiones province case, bureaucratic accreditation of land titles is postponed, the state is not committed to the realization of this right.RESUMEN: En este trabajo nos proponemos un acercamiento a las diversas ideas infantiles sobre el territorio habitado, en el contexto particular de la población mbyá guaraní de Misiones. La fragilidad territorialidad a la que es expuesta la población indígena en Argentina se manifiestan cotidianamente en diversas áreas. La población mbyá, ha sido surcada por los límites de los estados nacionales de Paraguay, Brasil y Argentina. La sedentarización y los cercamientos de tierras en los diferentes estados, han impulsado transformaciones de las lógicas de subsistencia, de habitación y organización comunitaria. En el caso de la provincia de Misiones la acreditación burocrática de los títulos de tierras se encuentra aplazada, el estado no se compromete con la concreción de este derecho.

  4. Consideraciones preliminares sobre la credibilidad

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    Fernando Verdugo

    2004-01-01

    Full Text Available A modo de introducción al Seminario Interno de Profesores de la Facultad de Teología de la PUC, el autor recuerda, en primer lugar, lo que suele entenderse por "credibilidad" y, también, algunos de los criterios generales por los cuales algo o alguien adquiere la calidad de creíble (el que sea razonable, relevante o significativo, capaz de producir gozo estético, etc.. Luego, en la parte más extensa, el autor vuelve a llamar la atención sobre la necesidad de atender a los condicionamientos culturales de la fe cristiana. La fe, al igual que toda experiencia humana, está culturalmente mediada. Tomar conciencia de ese hecho y asumir las consecuencias metodológicas es indispensable para una teología que quiere poner atención a los desafíos actuales y contribuir así a la credibilidad del cristianismoThis article reprints the introductory session to the internal seminar of lecturers from the Faculty of Theology of the UC. In the first place, the author sums up what is usually understood by `credibility' and the general criteria through which something or someone gains credibility (being reasonable, relevant or significant, capable of producing aesthetic pleasure. Then the author deals at length with the need to take notice of the cultural conditionings of the Christian faith. The faith, the same as any other human experience, is culturally mediated. It is crucial for theology to become aware of this fact and come to terms with its methodological consequences, if it wants to face current challenges and so contribute to the credibility of Christianity

  5. Is real-time PCR-based diagnosis similar in performance to routine parasitological examination for the identification of Giardia intestinalis, Cryptosporidium parvum/Cryptosporidium hominis and Entamoeba histolytica from stool samples? Evaluation of a new commercial multiplex PCR assay and literature review.

    Science.gov (United States)

    Laude, A; Valot, S; Desoubeaux, G; Argy, N; Nourrisson, C; Pomares, C; Machouart, M; Le Govic, Y; Dalle, F; Botterel, F; Bourgeois, N; Cateau, E; Leterrier, M; Le Pape, P; Morio, F

    2016-02-01

    Microscopy is the reference standard for routine laboratory diagnosis in faecal parasitology but there is growing interest in alternative methods to overcome the limitations of microscopic examination, which is time-consuming and highly dependent on an operator's skills and expertise. Compared with microscopy, DNA detection by PCR is simple and can offer a better turnaround time. However, PCR performances remain difficult to assess as most studies have been conducted on a limited number of positive clinical samples and used in-house PCR methods. Our aim was to evaluate a new multiplex PCR assay (G-DiaParaTrio; Diagenode Diagnostics), targeting Giardia intestinalis, Cryptosporidium parvum/Cryptosporidium hominis and Entamoeba histolytica. To minimize the turnaround time, PCR was coupled with automated DNA extraction (QiaSymphony; Qiagen). The PCR assay was evaluated using a reference panel of 185 samples established by routine microscopic examination using a standardized protocol including Ziehl-Neelsen staining and adhesin detection by ELISA (E. histolytica II; TechLab). This panel, collected from 12 French parasitology laboratories, included 135 positive samples for G. intestinalis (n = 38), C. parvum/C. hominis (n = 26), E. histolytica (n = 5), 21 other gastrointestinal parasites, together with 50 negative samples. In all, the G-DiaParaTrio multiplex PCR assay identified 38 G. intestinalis, 25 C. parvum/C. hominis and five E. histolytica leading to sensitivity/specificity of 92%/100%, 96%/100% and 100%/100% for G. intestinalis, C. parvum/C. hominis and E. histolytica, respectively. This new multiplex PCR assay offers fast and reliable results, similar to microscopy-driven diagnosis for the detection of these gastrointestinal protozoa, allowing its implementation in routine clinical practice.

  6. Dicty_cDB: Contig-U06058-1 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available rypanosoma cruzi strain Basileu c... 370 e-101 AJ536671_1( AJ536671 |pid:none) Solanum tuberosum mRNA for el...Blastocystis hominis isolate C elo... 370 e-101 AY785685_1( AY785685 |pid:none) T

  7. Research concerning intestinal parasitosis in infant population discovered in two clinic laboratories from Oradea (Romania

    Directory of Open Access Journals (Sweden)

    Daniel-Dumitru PORTELECHI

    2006-05-01

    Full Text Available Considering the research made and the data offered by the specialists in parasitology from Oradea’s no.1 Polyclinic and Children’s Hospital, we have established that children from 1 to 18 years old have been found infected with 7 species of human parasites. Giardia lamblia, Blastocystis hominis, Ascaris lumbricoides, Oxyurus vermicularis, Trichurus trichiura, Hymenolepis nana, Hymenolepis diminuta.

  8. DISERTACIÓN SOBRE JUSTICIA Y EQUIDAD

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    Sayuri P. Tamura M.

    2001-01-01

    Full Text Available «Disertación sobre equidad y justicia» es básicamente una respuesta a una inquietud en particular que yo tenía sobre las distintas posiciones que existen sobre estos dos temas y cómo definir cuál es la más acertada. Hice una descripción del punto de vista de Amartya Sen –la violación de los derechos sobre la propiedad privada es justificable si evitan las hambrunas2– frente al de Robert Nozick –la propiedad privada es un derecho fundamental y como tal debe ser respetado3–, sin embargo, aunque ambos enfoques son totalmente contrarios tienen mucho sentido, por lo cual es bastante difícil decidirse por uno en particular. Fue de esta forma, enfrentando sus opiniones, que yo logré desarrollar una posición frente a estas dos perspectivas, y así dar mi opinión al DISERTACIÓN SOBRE JUSTICIA Y EQUIDAD SAYURI P. TAMURA M. Estudiante de Economía y Negocios Internacionales de la Universidad ICESI. respecto. Al final, llegué a una conclusión, que aunque no es la más original y tampoco es la única, tiene mucho sentido y seguramente de ser posible, la situación del país podría cambiar para bien.

  9. Rastros: dizendo sobre os fazeres/dizeres

    OpenAIRE

    MELLO MARISOL BARENCO CORRÊA DE

    2001-01-01

    Com vistas ao aclaramento de noções erroneamente herdadas do contexto social e histórico da cultura moderna ocidental, que teve/tem como um de seus projetos o "apagamento" do sujeito, ou o distanciamento entre os sujeitos e suas formas de fazer, dizer, pensar, este artigo pretende contribuir com elementos para a construção de um outro paradigma nas ciências humanas. Trata-se de um relato sobre o processo de construção de nossa própria pesquisa, articulado à reflexão sobre as diversas formas d...

  10. Sobre la esclavitud: libro de artista

    OpenAIRE

    MACHADO JIMÉNEZ, ALBERTO

    2015-01-01

    Sobre la esclavitud es un libro de artista, donde encontraremos imágenes acompañadas de breves frases que hacen referencia al tema propuesto en cada ilustración para que así se pueda comprender mejor la intención de este trabajo, reflexionar sobre la manera que tenemos de ver las cosas, en este caso, con respecto a la esclavitud. Dar a conocer distintas formas de ser esclavo en este sistema sería uno de los objetivos principales. Hacer litografías, dibujos con el uso de acua...

  11. Blastocistosis y otras infecciones por protozoos intetinales en comunidades humanas ribereñas de la cuenca del rio Valdivia, Chile Blastocystosis and other intestinal protozoan infections in human riverside communities from - Valdivia River Basin, Chile

    Directory of Open Access Journals (Sweden)

    Patricio Torres

    1992-12-01

    Full Text Available Entre marzo y octubre de 1987 se examinaron muestras coprológicas de 970 personas (20, 9% de la población pertenecientes a 209 grupos familiares de las comunidades ribereñas de la cuenca del rio Valdivia, Chile; con el propósito de determinar las prevalencias de infección por Blastocystis hominis y otros protozoos intestinales para establecer relaciones con la edad y sexo de los hospedadores, saneamiento ambiental y porcentaje de individuos infectados por grupo familiar. Un 72, 5% de las personas presentó una o más especies de protozoos intestinales. La mayor prevalencia se registró para B. hominis (61, 8%, que se incrementó con la edad del hospedador al igual que en las infecciones por Endolimax nana y Entamoeba coli. No se demostró asociación entre el sexo del hospedador y la prevalencia de infección por B. hominis y otras especies de protozoos. La prevalencia de B. hominis fue mayor en individuos que habitaban viviendas cuya disposition de excrementos era no sanitaria. Más del 60% de los integrantes de los grupos familiares presentaron infección por B. hominis en el 53, 1% de las familias encuestadas en contraposición al 2,4%-21,8% observado en infecciones por otros protozoos. El examen de 45 muestras de excrementos de cerdos, reveló infección por Blastocystis en el 22,2% de estos animales.Between March and October 1987, the prevalence of infection by Blastocystis hominis and other intestinal protozoan, their relationship with the age and sex of the hosts, and the percentage of in fected persons in family groups were determined in riverside communities of Valdivia River Basin, Chile. One or more intestinal protozoan species were determined in 72.5% of the examined persons. The prevalence was greater for B. hominis (61.8%. The prevalences of B. hominis, Endolimax nana and Entamoeba coli were greater in relation to the age of the host. The sex of the host and prevalence of infections by B. hominis and other species of

  12. Consideraciones sobre diferentes procesos productivos y sus consecuencias sobre el medio ambiente

    OpenAIRE

    Caridad Ramos Alvariño

    2004-01-01

    En el presente trabajo se exponen algunas consideraciones sobre diferentes procesos productivos y sus consecuencias sobre el medio ambiente. La disminución del impacto negativo de algunas producciones sobre el medio ambiente es favorable, pero el problema va más allá y de lo que se trata es de la necesidad de una transformación sostenible de las producciones que puedan conducir a una vida agradable y una naturaleza conservada entre otras muchas cuestiones que agobian hoy al mundo. La solución...

  13. Las fuentes cristianas sobre Jesús

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    Melero Martínez, J.M.

    2010-01-01

    Full Text Available El artículo es una presentación de las fuentes históricas sobre Jesús de Nazaret: fuentes sinópticas, fuente Q, fuentes afines a la gnosis, fragmentos de evangelio con material sinóptico o joánico, evangelios judeocristianos y otras fuentes.

  14. Sobre un sistema no lineal de epidemias

    OpenAIRE

    Ortega S., Luis

    2012-01-01

    Un modelo sobre epidemias que describe la población infectada y la población susceptible de ser infectada en el cual se toma en consideraci6n el efecto de difusión es el siguiente: [Formula Matemática].

  15. Puente pretensado sobre el Guadarrama. Villalba

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    Páez, A.

    1958-12-01

    Full Text Available En este artículo se describe y comenta la solución dada al puente de hormigón pretensado sobre el río Guadarrama, primero de los construidos mediante dovelas prefabricadas y solidarizadas después, mediante el pretensado de la armadura longitudinal.

  16. Sobre o natural e o actor

    OpenAIRE

    Tavares, Margarida

    2010-01-01

    Neste artigo, procura-se reflectir sobre o conceito de natural quando aplicado ao trabalho do actor. Parte-se de quatro relevantes textos da dramaturgia ocidental, a saber: Hamlet, de Shakespeare; Crítica da ‘Escola das mulheres’ e O improviso de Versalhes, de Molière; e O teatro cómico, de Goldoni.

  17. Observaciones sobre didáctica

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    Alfonso Tamayo Valencia

    2014-02-01

    Full Text Available  En el presente artículo, a partir de la conceptualización sobre didáctica y enseñanza, se plantean sus posibilidades en la educación superior, especialmente cuando reflexionamos en las prácticas de enseñanza desde un saber hacer que supere el hacer de esquemas tácitos y espontáneos que caracteriza en buena parte docencia universitaria. Desde esta perspectiva se aboga por la renovación de la concepción de la didáctica desde una postura epistemológica y psicológica sobre la manera como se construye el conocimiento.

  18. Consideraciones sobre la motricidad del violinista

    OpenAIRE

    Allende, Pablo

    2009-01-01

    Comienza la exposición hablando de que intenta prevenir las patologías en los violinistas mediante secuencias de ejercicios específicos, de diferentes técnicas corporales y fisioterapéuticas. Presenta datos estadísticos sobre el índice de patologías que se encuentran entre estos intérpretes para por último presentar tablas de ejercicios propiamente dichas para intentar paliar estas dolencias

  19. Productos derivados sobre bienes de consumo

    OpenAIRE

    Francisco Venegas Martinez; Salvador Cruz Ake

    2010-01-01

    Este trabajo de investigacion desarrolla un modelo de equilibrio general con expectativas racionales en tiempo continuo util para la determinacion de precios de contratos forward, contratos futuros, bonos cupon cero y opciones europeas (de compra y venta) sobre bienes de consumo. Para ello, el modelo considera un individuo representativo en una economia con dos bienes, los cuales son producidos con tecnologias estocasticas. Por ultimo, el modelo propuesto permite examinar estrategias para obt...

  20. Reflexiones sobre el concepto de justicia

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    Víctor C. Pascual Planchuelo

    2005-06-01

    Full Text Available Este ensayo constituye una reflexión sobre el concepto de Justicia. No se centra, como tantos estudios al respecto, en el plano teórico y metafísico, sino que atiende problemas de mayor actualidad: la opinión de los ciudadanos, la postura de los profesionales y otros asuntos controvertidos del ámbito del Derecho.

  1. Neopolares de problemas de empaquetamiento sobre semigrupos

    OpenAIRE

    Aráoz Durand, Julián Arturo

    1982-01-01

    Los neopolares permiten caracterizar las caras de un poliedro combinatorio como vértices de poliedros altamente estructurados. Esto sirve para generar planos de cortes y para obtener propiedades duales en problemas de programación entera. Gomory caracterizó neopolares para problemas sobre grupos, Aráoz en "Polyhedral Neopolarities" extendió estos resultados a semigrupos de cubrimiento. En este trabajo se caracterizan neopolares importantes de semigrupos de empaquetamiento que incluyen los pro...

  2. Relationship between ParE gene mutation and fluoroquinolones resistance in Mycoplasma hominis%人型支原体ParE基因突变与耐氟喹诺酮类药物的关系

    Institute of Scientific and Technical Information of China (English)

    罗永慧; 段颖卿; 甘雪华; 王旭菲; 舒向荣

    2014-01-01

    目的:探讨人型支原体对氟喹诺酮类药物的耐药机制。方法自临床分离的8株耐氟喹诺酮类药物的人型支原体(Mh),对其ParE基因PCR扩增后进行测序分析,与基因库中的野生型菌株MHPG21基因序列比对,分析ParE基因突变位点与菌株耐氟喹诺酮类药物的关系。结果与野生株MHPG21对比,6株检出ParE基因所编码的氨基酸残基发生D 426→N变异。结论 Mh临床分离株对氟喹诺酮类药物的耐药可能与ParE基因所编码的氨基酸残基D 426→N变异有关。%Objective To explore the fluoroquinolones resistance meachanisms of Mycoplasma hominis (Mh). Methods A total of 8 strains of Mh with different fluoroquinolones resistance phenotype were analyzed, the ParE gene was amplified and sequenced. The sequencing results of ParE gene were compared with that in the wild type strain PG21, then analyzed the relation between ParE gene mutation and fluoroquinolones resistance. Results Compared with wild strain PG21, ParE gene in 6 strains got a D426→N mutation. Conclusion The fluoroquinolones resistance of Mh might be associate with ParE gene D426→N mutation.

  3. Comentario sobre levantar la mano sobre uno mismo, de jean améry

    OpenAIRE

    Pilar González Rivera

    2010-01-01

    Resumen En el libro de Jean Améry (seudónimo de Hans Mayer), Levantar la mano sobre uno mismo. Discurso sobre la muerte voluntaria, el autor utiliza argumentos de orden filosófico y suministra algunos escasos datos autobiográficos (como la aterradora experiencia sufrida por él al haber sido víctima de la persecución y tortura nazis en dos ocasiones, debido a su condición de hijo de padre judío) para justificar la “muerte voluntaria”, como llama al sui...

  4. ESTUDO SOBRE O IMPACTO DOS PROCESSADORES HOSPEDEIROS

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    Alba S. B. Lopes

    2008-05-01

    Full Text Available Arquiteturas reconfiguráveis são dispositivos de hardware capazes de modificar sua estrutura de hardware de acordo com a aplicação a ser executada. Tais dispositivos surgiram como uma alternativa às soluções de hardware tradicionais na tentativa de equilibrar flexibilidade e desempenho. Uma das principais formas de utilização de arquiteturas reconfiguráveis corresponde a um bloco reconfigurável anexado a um processador hospedeiro. Esse processador é responsável por executar diversas tarefas críticas, incluindo o particionamento hardware/software e indicar o momento do bloco reconfigurável executar. Apesar de existirem diversas propostas de arquiteturas reconfiguráveis na literatura, pouco se estudou sobre o impacto do processador hospedeiro na arquitetura. Nesse contexto, esse artigo apresenta um estudo sobre o impacto do processador hospedeiro no desempenho da arquitetura reconfigurável híbrida. Como estudo de caso foi realizada uma avaliação sobre o uso dos processadores SPARC V8 da Sun Microsystems e Nios II da Altera como processadores hospedeiros da arquitetura reconfigurável híbrida RoSA. O objetivo deste estudo é realizar a comparação entre o desempenho desses processadores e identificar qual deles é o mais adequado para anexar a arquitetura em questão. Os resultados mostraram que foi possível alcançar até 47% de ganho de desempenho com o uso do processador Nios II em comparação ao SPARC V8, indicando o primeiro como mais adequado para a arquitetura RoSA. PALAVRAS-CHAVE: arquiteturas reconfiguráveis, processador hospedeiro, desempenho.

  5. Literatura infantil sobre problemas infantiles: la diabetes

    OpenAIRE

    Lázaro Fernández, Patricia

    2015-01-01

    Debido al creciente número de casos de diabetes infantil que se está dando en la sociedad actual, no es raro encontrarse en las aulas cada vez más casos de esta enfermedad. Por esta razón, he decidido analizar dos libros sobre esta temática, para hacer un acercamiento del material seleccionado a las aulas y poder desarrollar junto con los niños un aprendizaje colectivo y cooperativo. En este trabajo se expone la importancia de la literatura y la literatura infantil como una ...

  6. Claves sobre el poder de las mujeres

    OpenAIRE

    García de León Álvarez, María Antonia

    2012-01-01

    El objeto de este texto es esbozar una síntesis sobre algunos nudos de mi obra en el área de Género y Poder. El estudio del poder es un punto de partida ineludible para abordar todo tema de Género, es decir, es necesario arrancar desde la comprensión del fenómeno social del poder, ya que el Patriarcado es un sistema estructurado de poder por género. Este punto de partida indica la relevancia del tema (nada más ni nada menos que el poder, tema clave en las Ciencias Sociales).

  7. Mapping sobre arquitecturas heterogéneas

    OpenAIRE

    De Giusti, Laura Cristina

    2008-01-01

    La programación de algoritmos paralelos, a diferencia de los algoritmos secuenciales, no cuenta para su representación con un único modelo. Esa carencia ocasiona que su aplicación sobre una arquitectura heterogénea, a través de modelos existentes, no halle el resultado que se ambiciona. La computación paralela ha propuesto, como pocas, significativos quiebres en el ámbito de la Ciencia de la Computación, al provocar la reformulación y generación de nuevos conceptos. El desarrollo de nuevos mo...

  8. Apuntes Sobre el Cultivo del Cacao.

    Directory of Open Access Journals (Sweden)

    Ochoa Horacio Ochoa

    1940-12-01

    Full Text Available En el presente artículo se hablara del proceso de fermentación del cacao, pero antes de entrar a detallar este proceso, es conveniente dar algunas anotaciones sobre los sistemas de recolección o “cosecha” empleados en distintos lugares, pues ellos tienen sus diferencias y en ocasiones no se efectúan con todo el cuidado necesario tanto para el árbol como para el producto que irá a los depósitos de fermentación.

  9. Futuros: estudo de caso sobre futuros cambiais

    OpenAIRE

    Santo, Paula Cristina Rosa da Silva Espírito

    2010-01-01

    Mestrado em Contabilidade A presente dissertação foi realizada com vista à obtenção do grau de mestre em Contabilidade pelo Instituto Superior de Contabilidade e Administração de Lisboa. Para o desenvolvimento desta dissertação foi escolhido o tema “Contratos de Futuros”. Em termos estruturais esta monografia divide-se em duas partes. Na primeira parte faz-se uma exposição sobre o tema os “Futuros”. Na segunda parte é apresentado o trabalho de investigação que foi desenvolvido. Na...

  10. Algunas reflexiones sobre la supervivencia empresarial

    OpenAIRE

    Sarabia Alzaga, José María

    1995-01-01

    Dos bloques-tópico, bien conocidos, configuran el esquema lógico de las reflexiones que se proponen. El primero de ellos se centra en nuestra realidad global: el entorno; el segundo, en los impactos que éste ha generado en la empresa, exponiendo una síntesis contrastada -y hoy día vigente- sobre cómo las empresas de éxito están consiguiendo sobrevivir. Como conclusión, se sintetizan algunas ideas o lineas generales de actuación que pudieran ayudar a mejorar o reforzar las propias experiencias...

  11. Sentencias Constitucionales sobre la muerte digna

    OpenAIRE

    Serrano, J. M.

    2006-01-01

    La sentencia comentada ha sido considerada como relevante en la discusión sobre el derecho a la vida en relación con la eutanasia. Sin embargo, las peculiaridades del caso qeu le dio origen y la propia argumentación del tribunal la alejan d euna interpretación relevante en torno a los dos tópicos del derecho a la vida y a la dignidad. Por el contrario la sentencia resulta decepcionate en su argumentación más propia. Primero respecto a la propia huelga de hambre que condena sin matices en rela...

  12. Consideraciones sobre de la ética

    OpenAIRE

    Enrique Cantolla Bernal

    2005-01-01

    El presente ensayo muestra como el hombre, a través de la historia, a estado constantemente buscando beneficios para su vida y ha debido cumplir con varias tareas, que con el tiempo han dado origen a costumbres y comportamientos que han llevado a constituir la moral y la ética. Esta última, nos permite definir y determinar los principales fines de la vida del hombre y cuales son los principios y normas que lo rigen. Así, el estudio de la ética nos plantea la interrogante sobre "los fines de l...

  13. Puntualizaciones sobre el concepto de latencia

    OpenAIRE

    Bortolazzo, Antonela

    2007-01-01

    El presente escrito revisa un concepto elaborado por Freud, como es la noción de Período de latencia sexual. Antes de hacer explícito el enfoque desde el cual será abordado quisiera mencionar que, lo que a continuación se desarrolla se enmarca en el trabajo de investigación que sobre el concepto de Latencia realiza, desde hace catorce años, la Cátedra de Psicología Evolutiva I, de la cual formo parte. En esta oportunidad me propongo indagar el concepto de latencia en el marco de la teoría ...

  14. Notas sobre envidia, equilibrio y optimalidad

    OpenAIRE

    Cristini, Marcela; Chisari, Omar Osvaldo

    1980-01-01

    Demostramos aquí -aún para coeficientes de producción distintos de la unidad- la suficiencia de las características del equilibrio para determinar el efecto aludido sobre la situación de ambos agentes. Damos otra condición suficiente, que no supone necesariamente el equilibrio; generalizamos los resultados a un número mayor de consumidores y deducimos propiedades del óptimo de Pareto, definido como aquella situación en la que no es posible mejorar el nivel de utilidad de un agente sin reducir...

  15. Efecto de masticar chicle sobre la halitosis

    OpenAIRE

    De Luca Monasterios, Fiorella; Chimenos Küstner, Eduardo; López López, José

    2014-01-01

    Fundamento y objetivo: Este estudio pretende estimar la prevalencia de halitosis oral en una población joven y mostrar el efecto del chicle sobre el aliento. Pacientes y método: Estudio prospectivo de campo descriptivo correlacional, de corte transversal. Se seleccionaron a conveniencia 121 jóvenes, de los que 98 concluyeron el estudio, el cual se realizó en el Hospital Odontológico Universitario-Universidad de Barcelona. El protocolo consistió en: cuestionario, evaluación clínica oral, prueb...

  16. El tullido. Un cuento sobre los cuentos

    OpenAIRE

    Fernández-Urtasun, R. (Rosa)

    2005-01-01

    Este artículo realiza una lectura literaria y simbólica de El tullido, uno de los cuentos de Christian Andersen que pasó desapercibido entre los relatos que se publicaron tras su muerte. El relato del argumento va acompañado de una reflexión sobre los temas que aparecen recogidos en el texto: las tradiciones orales, la belleza del arte, la esperanza, la creación, la importancia de la escritura y de la lectura, la esencia de la literatura.

  17. Ensayo sobre la melancolía

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    Marta Chacón Salinas

    2013-07-01

    Full Text Available Este trabajo es un intento de delimitar la melancolía del conjunto de los trastornos depresivos y de reflexionar sobre sus características tal como aparecen en el discurso de los melancólicos. Se consideran algunas hipótesis en la línea psicoanalítica freudiana y lacaniana (Lambottc, Kristeva para entender cómo se va constituyendo en sus características principales: la intolerancia a la pérdida del objeto y el desfallecimiento del símbolo que le impide elaborar este duelo.

  18. Recursos sobre propiedad intelectual en internet

    OpenAIRE

    Sorli Rojo, Ángela; Merlo Vega, José Antonio

    2001-01-01

    El concepto de propiedad intelectual, entendido en su sentido más amplio, englobaría todos los aspectos relacionados con los derechos que se obtienen de una creación, tanto en su forma original, como de sus posibles reproducciones y transformaciones. En este trabajo se recopilan y analizan una serie de fuentes de información accesibles en Internet sobre este tema. No obstante, la idea de propiedad intelectual no coincide plenamente con la de propiedad industrial, que es mucho m...

  19. Productos derivados sobre bienes de consumo

    OpenAIRE

    Francisco Venegas Martínez; Salvador Cruz Ake

    2010-01-01

    Este trabajo de investigación desarrolla un modelo de equilibrio general con expectativas racionales en tiempo continuo útil para la determinación de precios de contratos forward , contratos futuros, bonos cupón cero y opciones europeas (de compra y venta) sobre bienes de consumo. Para ello, el modelo considera un individuo representativo en una economía con dos bienes, los cuales son producidos con tecnologías estocásticas. Por último, el modelo propuesto permite examinar estrategias para o...

  20. Avances sobre bases de datos no convencionales

    OpenAIRE

    Arroyuelo, Jorge; Esquivel, Susana Cecilia; Grosso, Alejandro; Ludueña, Verónica; Reyes, Nora Susana; Chávez, Edgar; Navarro, Gonzalo

    2014-01-01

    En la actualidad han surgido, debido a los avances alcanzados en tecnologías de información y comunicación, aplicaciones no tradicionales sobre bases de datos de todo tipo de datos, valga la redundancia. Éstos pueden ser imágenes, texto libre, secuencias de ADN, audio, video, etc., pueden estar o no estructurados y provenir de diversas fuentes (satélites, telescopios, revistas, fotografías, música, etc.), como también ser de diferentes tamaños, generalmente muy grandes. Por otro lado, las con...

  1. Soportes apoyados sobre vigas pretensadas (EE. UU.

    Directory of Open Access Journals (Sweden)

    Editorial, Equipo

    1965-12-01

    Full Text Available This work has been awarded a prize by the Prestreesed Concrete Institute. It refers to a number of flyovers on the Arroyo Seco, of California. This river has been canalized, to help drainage in case of heavy rainfall. The canal runs through a highly populated zone, and a motor road and accessory ramps had to cross the canal. The resulting design involves three prestressed girders, lightly arched in a parabolic outline, resting on each river shore, running along the line of the bridge, over the canal. It was decided that the bridge piles should not rest on the river bed, in order not to reduce its flow capacity. The girders of a maximum length of 39 ms, are box shaped, and stiffened with thin diaphragms. The cylindrical pile, 1.50 m in diameter, is an integral part of the girder, and applies to it a concentrated load of about 4000 tons. The girders rest on reinforced concrete cubes, which in turn are supported by concrete slabs and piles driven into the soil.Esta obra ha sido premiada por el Prestressed Concrete Institute y se refiere a una serie de pasos superiores sobre el Arroyo Seco, en California, que se ha canalizado para servir de desagüe en el caso de avenidas torrenciales. El canal se halla en una zona de edificación densa y se trata del paso—sobre aquél—de una autopista y rampas de acceso. La solución adoptada consistió en tres vigas pretensadas, ligeramente arqueadas con perfil parabólico, apoyadas en las dos márgenes y situadas en correspondencia con los tres soportes, sobre el propio canal, ya que ninguno de aquéllos debía apoyarse en el lecho de este último al objeto de no disminuir su capacidad de desagüe. Las vigas—de unos 39 m de longitud la mayor—son del tipo cajón, rigidizadas con unos diafragmas de pequeño espesor. El soporte cilíndrico, de 1,50 m de diámetro, se solidariza con la viga y le transmite una notable carga concentrada, del orden de 4.000 toneladas. Los apoyos de estas vigas son simples dados

  2. Reflexiones sobre contabilidad privada y contabilidad nacional

    OpenAIRE

    José Arturo Gutiérrez

    1995-01-01

    Se discuten las interrelaciones de la contabilidad privada y la contabilidad nacional, insistiendo en sus condicionantes internos contradictorios en relación con la sobredeterminación de la formación económico-social vigente. En relación con ello se introduce la reflexión sobre la unidad contradictoria entre empresa y capital, esto es, la base técnica frente a su determinante socioeconómico, conflicto que la propia contabilidad privada expresa: al describir el capital aporta necesariamente el...

  3. Tributos sobre a folha ou sobre o faturamento? Efeitos quantitativos para o Brasil

    Directory of Open Access Journals (Sweden)

    Tiago V Cavalcanti

    2008-09-01

    Full Text Available Este artigo estuda os impactos alocativos e de bem-estar de uma reforma tributária que substitui os impostos sobre a folha de salários por outro que incide sobre a receita. Para isso, desenvolve-se um modelo neoclássico de crescimento econômico com impostos sobre a receita e sobre a folha de salários. As simulações com parâmetros da economia brasileira mostram que tal reforma tributária tem um efeito negativo no bem-estar, no estoque de capital de longo prazo e consequentemente no produto per capita de longo prazo. Contudo, o emprego aumenta.This paper studies the impacts of a tax reform which substitutes taxes on labor income by taxes on total revenue. In order to accomplish that, we construct and solve a general equilibrium neoclassical growth model with taxes on labor income and total revenue. The numerical simulations with Brazilian parameters show that such tax reforms would negatively affect welfare, the capital stock, and consequently per capita output. However, employment would increase. Such effects are quantitatively important.

  4. Consideraciones criminológicas sobre las bandas juveniles

    OpenAIRE

    Medina, Juanjo, Ralphs, Robert, Aldridge, Judith

    2010-01-01

    Este artículo ofrece una serie de reflexiones originales sobre la definición de las bandas juveniles y resume algunos de los debates claves en el campo de investigación sobre bandas. En particular, se presta especial atención a los estudios sobre la relación entre bandas y delincuencia, las teorías que explican la formación de las bandas y los factores que empujan a los jóvenes a vincularse a las mismas, los estudios sobre respuestas de política criminal a este fenómeno, ...

  5. Sobre los límites

    Directory of Open Access Journals (Sweden)

    Walter Cabrera-Febola

    2014-10-01

    Full Text Available El intercambio de información entre entes naturales se produce a través de los boundaries [límites], este hecho reclama un estudio sistemático de ellos, lo cual hasta el momento no se ha realizado. En el presente trabajo, establecemos el concepto general de boundary, para luego identificar ciertos tipos de boundaries como interface, límite, borde y algunos de sus tipos, y barrera son identificados y conceptualizados. Luego realizamos un estudio parcial de algunos de los boundaries conceptualizados, tratando de analizarlos lo más posible. Finalmente, se da una discusión general sobre la existencia e importancia de los boundaries remarcando la generalidad y robustez del concepto de boundary y sus diferentes tipos, de aquí su utilidad en la ciencia y la tecnología es posible de apreciarse. Por último se comenta sobre su perspectiva en ciencia y tecnología.

  6. Detection of parasites in children with chronic diarrhea.

    Science.gov (United States)

    Maçin, Salih; Kaya, Filiz; Çağdaş, Deniz; Hizarcioglu-Gulsen, Hayriye; Saltik-Temizel, Inci Nur; Tezcan, İlhan; Demir, Hülya; Ergüven, Sibel; Akyön, Yakut

    2016-06-01

    The aim of this study was to investigate the frequency of intestinal parasites in patients with chronic diarrhea and clarify the importance of these parasitic pathogens in such cases. A total of 60 pediatric patients with chronic diarrhea between June 2012 and October 2014 were enrolled in the study. Out of 60 stool samples, five were positive for Giardia lamblia, two, Dientamoeba fragilis, and one, Blastocystis hominis. One stool sample was positive for Entamoeba hartmanni and B. hominis, another one was positive for G. lamblia and B. hominis, another, G. lamblia and E. hartmanni and one sample was positive for Enterobius vermicularis, D. fragilis and B. hominis together. Parasitic infection, which decreases quality of life and increases susceptibility to other infections, should not be neglected, particularly in patients with chronic diarrhea. Accurate diagnosis decreases morbidity and mortality in patients with parasite infection. PMID:27322863

  7. Hábitos de consumo sobre la cerveza

    OpenAIRE

    Velasco Matilla, Débora

    2015-01-01

    Estudio de los hábitos de consumo de cerveza en un pueblo determinado situado en la provincia de Segovia, Cantalejo. Contiene un resumen sobre la historia de la cerveza y su tipología y un estudio de mercado sobre la cerveza partiendo de una encuesta. Grado en Comercio

  8. Violência sobre Crianças

    OpenAIRE

    Sacadura, Catarina Brito; Aragão, Maria José

    2014-01-01

    Trata-se de um estudo de revisão sobre a violência exercida sobre crianças, os agentes da violência, os sujeitos da violência, os factores de risco, as consequências na idade adulta e a recidividade de violência sexual.

  9. Algunas observaciones sobre el mundo actual

    OpenAIRE

    Tello, Ángel Pablo

    1993-01-01

    El artículo expone las nuevas condiciones políticas creadas en el mundo a partir de la caída del Muro de Brelín y la disolución de la Unión Soviética. Desde allí, el autor intenta reflexionar sobre las líneas de acción que emergen en el planeta como consecuencia de ello y de la ausencia de referencias globales que se observaron durante décadas. En particular, el ascenso de los nacionalismos que no sólo es un fenómeno que afecta a los países en desarrollo, sino también a las potencias industri...

  10. Algunas reflexiones sobre el turismo cultural

    Directory of Open Access Journals (Sweden)

    Claudia Toselli

    2006-01-01

    Full Text Available El presente artículo, si bien no pretende hacer un análisis exhaustivo sobre el turismo cultural, tiene por objetivo presentar algunas reflexiones acerca de esta modalidad turística desde diferentes aspectos. En primer lugar se abordará la relación entre turismo cultural y globalización, para luego centrarse en la incidencia de esta actividad como factor de desarrollo local y regional, haciendo referencia a algunos ejemplos de Argentina. Intentará también introducir acerca de los aspectos positivos y negativos, o puntos fuertes y débiles del turismo cultural. Y por último, esbozará algunas consideraciones acerca del rol de las organizaciones internacionales y de las principales agencias de cooperación internacional, incluyendo las resoluciones, declaraciones y recomendaciones más significativas en este campo del turismo.

  11. NOTAS SOBRE A LÓGICA ESTOICA

    Directory of Open Access Journals (Sweden)

    Valter Duarte Moreira Júnior

    2014-02-01

    Full Text Available Diante do grande desenvolvimento da Lógica e do cálculo proposicional no século XX, estudiosos do assunto em todo o mundo se concentraram em um rigoroso estudo dessa disciplina e sua história. Nesse processo, o eminente lógico polonês Jan Lukaziewicz descobriu em sua pesquisa sobre história da lógica a Lógica Estoica, que, já em III a. C., havia antecipado diversos métodos e conceitos da Lógica Moderna. Nossa intenção neste artigo é fazer um breve esboço da lógica desenvolvida pelos Estoicos.

  12. Reflexiones sobre el tratamiento ambulatorio de drogodependientes.

    Directory of Open Access Journals (Sweden)

    Juan Carlos González García

    1989-01-01

    Full Text Available Intentamos plantear algunas de las innumerables contradicciones, paradojas y mitos que se presentan en relación con las demandas y con las necesidades terapéuticas de los consumidores de drogas, desde el nivel de atención extrahospitalaria pública y dentro de una Unidad de Salud Mental. Se intenta reflexionar sobre los conocimientos teóricos y la realidad práctica, su interdependencia, complementariedad y divergencia, con la finalidad de evitar la huida asistencial o la omnipotencia terapéutica. Se valoran las diferencias existentes entre la farmacología teórica, anclada en el paradigma lewiniano, y la realidad clínica.

  13. Conceptos fundamentales sobre el mantenimiento de edificios

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    Juan Miguel Arencibia Fernández

    2007-01-01

    Full Text Available En esta investigación se realiza una profunda búsqueda sobre el tema del Mantenimiento de Edificios donde se abundan diferentes conceptos y criterios de la misma, encontrándose de esta forma los más utilizados métodos a emplear. Luego teniendo en cuenta las clasificación del Mantenimiento de Edificio según los tipos de obras, propietarios del inmueble y el momento que éste se realiza, como los costos de una edificación y su equipamiento a utilizar. Otros de los aspectos a tener en cuenta es la organización del Mantenimiento su planificación y responsabilidad, las ventajas que puede traer tanto para la economía y para el mismo usuario, como su vida útil, para el cual fueron ejecutadas las edificaciones.

  14. Sobre a moralidade do direito em Kant

    Directory of Open Access Journals (Sweden)

    Joãosinho Beckenkamp

    2009-07-01

    Full Text Available http://dx.doi.org/10.5007/1677-2954.2009v8n1p63Na atual literatura sobre a fi losofi a prática de Kant há uma tendência a negligenciar a distinção feita na Metaphysik der Sitten entre direito (Jus e ética (Ethica, a ponto de ser a doutrina do direito de Kant vista como uma mera aplicação do imperativo categórico. Este artigo procura mostrar que Kant apresenta em sua doutrina do direito uma série de argumentos que logram estabelecer o direito de modo inteiramente independente da ética.

  15. Neruda e Drummond: um olhar sobre Stalingrado

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    Moacir Lopes de Camargos

    2015-07-01

    Full Text Available Este artigo tem por objetivo promover uma aproximação entre dois expoentes da lírica latino-americana do século XX, a saber: Pablo Neruda e Carlos Drummond de Andrade, a partir da análise dos poemas Canto a Stalingrado (1942 e Carta a Stalingrado (1943. Apresentaremos a visão histórica desses poetas sobre a Batalha de Stalingrado, considerada um dos principais eventos da Segunda Guerra Mundial. Ademais, será abordada a questão do engajamento político, que envolve a produção poética dos referidos autores.

  16. NOTAS SOBRE A LÓGICA ESTOICA

    OpenAIRE

    Valter Duarte Moreira Júnior

    2014-01-01

    Diante do grande desenvolvimento da Lógica e do cálculo proposicional no século XX, estudiosos do assunto em todo o mundo se concentraram em um rigoroso estudo dessa disciplina e sua história. Nesse processo, o eminente lógico polonês Jan Lukaziewicz descobriu em sua pesquisa sobre história da lógica a Lógica Estoica, que, já em III a. C., havia antecipado diversos métodos e conceitos da Lógica Moderna. Nossa intenção neste artigo é fazer um breve esboço da lógica desenvolvida pelos Estoicos....

  17. Apuntes sobre el Cultivo del Cacao

    Directory of Open Access Journals (Sweden)

    Ochoa Horacio

    1940-03-01

    Full Text Available Por muchos años permaneció el cultivo del cacao en nuestro país, en un deplorable atraso técnico; el agricultor a duras penas si tenía conocimientos generales de su explotación y, de una industria que tuvo su auge debido a la extensividad de las plantaciones, hoy sólo resta una reducida porción de ella. ¿Qué factores operaron esa tan notoria reducción hasta el punto de que exportadores en otros tiempos nos convertimos en importadores obligados de ese producto? Hay muchas razones de orden técnico y de orden económico. Los cacaotaleros obtenían halagadoras retribuciones cuando el jornal consistía en la alimentación que se le daba a la peonada y cuando, generoso, añadía una sobretasa en dinero. Bajo ese sistema de explotación, el descuadre que pudiera ocasionar tanto las enfermedades criptogámicas como los plagas, no era suficiente como para provocar el pesimismo en el hacendado y por tanto inducirlo al abandono de la plantación. A medida que las condiciones sociales del campesino se fueron modificando y su standard de vida aumentaba, cambió fundamentalmente el mecanismo de la industria Se observó entonces que los árboles morían y que era pobre la producción; que lo que en tiempos pasados fuera un negocio lucrativo, se les había convertido en una actividad improductiva y, para controlar estos fenómenos que se generalizaban en todas las zonas cacaotaleras, opusieron la ignorancia, el rutinarismo y la desidia. Lentamente se han ido modificando los conceptos antiguos sobre el trabajo cultural y los cuidados que con relación a las enfermedades y plagas requiere este delicado cultivo; la rutina está echando paso atrás y las experiencias científicas que procuran la prosperidad del plantío, se ponen en práctica en algunas zonas . Esto da esperanza para la reconstitución de la industria cacaotalera en nuestro país, que cuenta con inmensas regiones de óptima calidad para hacerla florecer sobre bases nuevas, con

  18. Sobre el Boletín

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    Andrés Arias

    2014-03-01

    Full Text Available En la página de Internet de la Biblioteca Luis Ángel Arango se indica que una de la funciones principales del Boletín es la de reseñar la novedades bibliográfica que van apareciendo en el país. "Novedades": de lo nuevo, tanto en las publicaciones como en las reseñas. Es decir, si lo que asegura la página de la Biblioteca es cierto, quien se acerca al Boletín debe encontrarse con comentarios recientes sobre libros recientes. Con guía que le ayuden a elegir entre las mil ofertas que hay en el mercado bibliográfico. Pero bien sabemos que este objetivo no se cumple.

  19. Aspectos da pesquisa sobre tipologia textual

    Directory of Open Access Journals (Sweden)

    Luiz Carlos Travaglia

    2014-03-01

    Full Text Available Este artigo chama a atenção dos pesquisadores sobre tipologiatextual, na área da Linguística Textual, sobre aspectos que sãoimportantes para que estas pesquisas gerem conclusões egeneralizações mais confiáveis. Os pontos comentados são: a anecessidade de uma teoria tipológica geral de textos que inclusivedetermine se as categorias de texto são todas da mesma natureza. Oautor propõe uma teoria geral; b a necessidade de estudos maishorizontalizados ou amplos que estabeleçam campos de pesquisa ede estudos mais verticalizados, que aprofundam o conhecimentode pontos específicos; c os aspectos metodológicos para superarproblemas no levantamento das categorias de texto existentes emuma sociedade e cultura, em suas diversas comunidades discursivase esferas de ação social; d a questão dos parâmetros e critérios paraconsiderar uma categoria de texto como sendo uma categoria e seela é de determinada natureza; e a questão dos critérios e parâmetrospara a caracterização das categorias de texto; f a necessidade deestudar as diversas relações de diferentes tipos que podem ocorrerentre as categorias de texto (de diferentes naturezas, tanto em suacomposição quanto em seu funcionamento sociodiscursivo; e g aatenção que merece a questão teminológica.

  20. El puente Bendorf sobre el Rin (Alemania

    Directory of Open Access Journals (Sweden)

    Hirschfeld, Kurt

    1966-03-01

    Full Text Available This recently completed bridge over the Rhine is 524 m long, and is a reinforced concrete structure, built by successive overhangs, without the aid of formwork or scaffolding. The centre span is 208 m in length, and the other six vary between 43 and 71 m. The deck consists of two independent parts, including a continuous box girder, with a hinge at the crown of the main span. It is stiffened with transversal diaphragms, spaced every 34 m. The piles rest on pneumatic caissons, which go down to 16 m below the river bed. The attachment between the piles and the caissons is rigid, at the central pile, and at the other piles it operates through a roller bearing. The central span was constructed without intermediate supports for the first 40 m, and beyond that stage, for greater safety, a provisional movable tower was built, as a temporary point of support for the free overhang. The various sections were concreted and post-tensioned with 32 mm bars, by the Dywidag procedure. Account was taken of the varying deformations that took place at each section, so as to add the necessary counter-deflection that finally enabled the two sides of the arch to meet at the crown at the calculated point.Este puente, recientemente construido, sobre el Rin, tiene una longitud total de 524 m, es de hormigón armado y se ha realizado por el procedimiento de voladizos sucesivos, sin entramado auxiliar ni cimbras. La luz del tramo central es de 208 m, y las de los otros seis oscilan entre 43 y 71 m. La estructura del tablero se compone de dos partes independientes, formadas por una viga tipo cajón, continua, con una articulación en la clave del tramo central y rigidizada con diafragmas transversales espaciados a 34 m. Las pilas centrales se apoyan sobre cajones neumáticos que descienden 16 m debajo del fondo del río. La unión entre estas pilas y la estructura cajón es rígida, y de tipo rodillo en las otras pilas. El tramo central se ha construido por voladizos

  1. Algunas reflexiones sobre el poder itinerante almohade.

    Directory of Open Access Journals (Sweden)

    Maribel FIERRO

    2009-12-01

    Full Text Available La primera parte del artículo consiste en la presentación de dos estudios sobre el poder itinerante en el Magreb: Jocelyne Dakhlia (1988 para las épocas hafsí y posteriores; Manuela Marín (2005 sobre el caso almohade. En la segunda parte, primeramente se analiza la convergencia del itinerario de Ibn Tumart con las conquistas de ‘Abd al-Mu’min: el movimiento –real o legendario– del fundador mesiánico del imperio marca el territorio conquistado por su sucesor el califa y su presencia inserta el espacio en la memoria, la geografía y el poder imperiales. A continuación, se plantea la compleja cuestión de la «capitalidad» almohade: se puede hablar de una capital sagrada (Tinmal, una capital central (Marrakech, capitales regionales (Sevilla, Túnez y de una nueva capital inacabada (Rabat. Pero es la presencia (hadar del propio califa la que determina dónde está el centro del imperio.La première partie de cet article présente deux études sur le pouvoir itinérant au Mahgreb : celle de Jocelyne Dakhlia (1986 sur la période hafside et les suivantes, celle de Manuela Marín (2005 sur le cas almohade.Dans la seconde partie, on analyse d’abord la convergence de l’itinéraire d’Ibn Tumart avec les conquêtes d’‘Abd al-Mu’min : le  trajet – réel ou légendaire – du fondateur messianique de l’empire marque le territoire conquis par son successeur le calife et sa présence ancre l’espace dans la mémoire, la géographie et le pouvoir impériaux. Ensuite, on examine la question complexe de la « capitalité » almohade : on peut parler d’une capitale sacrée (Tinmal, d’une capitale centrale (Marrakech, de capitales régionales (Séville, Tunis et d’une nouvelle capitale inachevée (Rabat. Mais c’est la présence (hadar du calife lui-même qui détermine où est le centre de l’empire.

  2. Sobre la rehabilitación de la arquitectura

    OpenAIRE

    Iglesia Salgado, Félix de la

    2012-01-01

    El presente ensayo reflexiona sobre el papel actual de la técnica de la arquitectura ante determinadas estrategias y comportamientos urbanos que, volcadas sobre nuevas situaciones resultado del cambio cualitativo y cuantitativo que se produce en nuestras ciudades, proponen una nueva urbanidad; una búsqueda, a partir de la rehabilitación del tejido residencial y los espacios de relación, sobre el control de los nuevos lugares y formas de habitabilidad que se hacen visibles como partes de una r...

  3. Pilas de arena sobre grafos dirigidos y algo de complejidad

    Directory of Open Access Journals (Sweden)

    Carolina Mejía Moreno

    2006-01-01

    Full Text Available En este artículo estudiamos el Modelo de Pilas de Arena sobre grafos dirigidos. El comportamiento del modelo sobre grafos dirigidos es más complejo (en término estrictos que sobre grafos no dirigidos; es por ello que, para muchas de las preguntas centrales de la teoría, no se conoce la respuesta en el caso dirigido. En este artículo se ha sintetizado la teoría para digrafos, se han simplificado algunas pruebas y se concretan algunos resultados relacionados con la complejidad de predicción del autómata.

  4. Pistas para pensar las significaciones imaginarias sobre la invariancia

    OpenAIRE

    Bilyk, Pablo Andrés

    2011-01-01

    Este escrito propone un primer esbozo de nominalizaciones sobre las significaciones imaginarias en torno a la idea de invariancia, con el fin de comenzar a mapear categorías para transitar que nos permitan pensar las construcciones fantasmáticas sobre el no-cambio. En definitiva, se trata de avanzar sobre la problematización de los modos de funcionamiento, constitución y reconfiguraciones de las hegemonías en los entramados sociales contemporáneos.

  5. Determination of recombination in Mycoplasma hominis

    DEFF Research Database (Denmark)

    Jacobsen, Iben Søgaard; Boesen, Thomas; Mygind, Tina;

    2002-01-01

    indicating the presence of recombination. In order to test for intergenic recombination, phylogenetic trees were reconstructed for each of the genes but no well-supported bifurcating phylogenetic trees could be obtained. The genes were tested for intragenic recombination using the correlation between linkage...

  6. A foodborne outbreak of Cryptosporidium hominis infection

    DEFF Research Database (Denmark)

    Ethelberg, S.; Lisby, M.; Vestergaard, L. S.;

    2009-01-01

    separate salad bar ingredients were found to be likely sources: peeled whole carrots served in a bowl of water, grated carrots, and red peppers (in multivariate analysis, whole carrots: OR 2.1, 95% CI 1.1-4.0; grated carrots: OR 2.1, 95% CI 1.2-3.9; peppers: OR 3.3, 95% CI 1.7-6.6). We speculate...

  7. Sobre a "conveniência" da escola

    Directory of Open Access Journals (Sweden)

    Marisa Vorraber Costa

    2009-01-01

    Full Text Available El artículo pretende mostrar un nuevo "uso" para la escuela, vinculado a la lógica de la etapa actual de la globalización en la que la cultura ocupa una posición singularmente importante, entrelazándose en la vida social, en los circuitos económicos y en los regímenes políticos. Recurriendo a la teorización de George Yúdice sobre los usos de la cultura en la era global, se analizan tres proyectos sociales - Você Apita, Tim Música nas Escolas y Escola Aberta - considerados expresivos de la forma como empresas, organizaciones públicas e iniciativas de la sociedad civil se articulan con la cultura y con la educación para hacer con que los mercados globalizados se muevan, pero no sólo en sus propios intereses, aunque ellos persistan en la cima de las prioridades. Hay indicios de que la "conveniencia" de la escuela sobrepasa el interés mercantil inmediato, y se proyecta como estrategia dirigida hacia una variada gama de propósitos socio-políticos, económicos y culturales. Entre ellos, la posibilidad de gobernar la vida de las personas, forjando visiones de mundo y de sociedad.

  8. sobre la población

    Directory of Open Access Journals (Sweden)

    Mauricio Schoijet

    2005-01-01

    Full Text Available En este artículo se analizan varios elementos de la obra de Thomas Malthus sobre población, en particular la recepción de su teoría y su posición política. Se resumen las críticas políticas, ideológicas y científicas que ha recibido, y se examinan asuntos hasta ahora no tratados por otros comentaristas, como es el caso de las excepciones admitidas por Malthus respecto al papel de las relaciones sociales feudales en la agricultura de Polonia y Rusia. Se sugiere que pese a que su teoría tendía a reforzar la pesada represión social y sexual existente, Malthus no fue apoyado por la burguesía británica (contrariamente a la apreciación de Marx. Se deduce que Malthus no percibió cabalmente los avances de la agricultura, y se refuta la tesis de Donald Winch de que habría sido un liberal de izquierda en política pues si bien en algunos aspectos se le puede considerar progresista, en otros, esenciales, conservador extremadamente represivo o protofascista, incluso precursor de la teoría de los golpes de Estado.

  9. Consideraciones sobre de la ética

    Directory of Open Access Journals (Sweden)

    Enrique Cantolla Bernal

    2005-12-01

    Full Text Available El presente ensayo muestra como el hombre, a través de la historia, a estado constantemente buscando beneficios para su vida y ha debido cumplir con varias tareas, que con el tiempo han dado origen a costumbres y comportamientos que han llevado a constituir la moral y la ética. Esta última, nos permite definir y determinar los principales fines de la vida del hombre y cuales son los principios y normas que lo rigen. Así, el estudio de la ética nos plantea la interrogante sobre "los fines de la vida en este mundo" y cuales son las conductas para conseguirlos. Sin embargo, el autor plantea que el hombre se enfrenta a la elección de valores alternativos y ante la interrogante de ¿cuál es el sumo bien del ser humano y de la sociedad?, han surgido diversas teorías que muestran como, casi todas las sociedades, han elegido la búsqueda de la felicidad como "el sumo bien", pero este concepto de "la felicidad" ha variando producto de las diversas teorías y doctrinas, que han emergido a lo largo de su historia.

  10. Apuntes sobre el Cultivo del Cacao

    Directory of Open Access Journals (Sweden)

    Chavarriaga. Eduardo

    1940-09-01

    Full Text Available El sombrío constituye uno de los problemas que se presentan en el cultivo del cacao, de necesaria resolución para ayuda del agricultor en su labor al producir tan importante grano. Contribuye a la economía del cultivo un conocimiento amplio de la cuestión que debe preocupar al productor inquiriendo no solamente por lo acostumbrado en la región, sino por lo que en otras partes se conoce sobre el particular, para abandonar un poco el emperismo y hacer una industria cada día más próspera. Como razón a favor del sombrío, tenemos los países en donde se produce cacao de fina calidad; Trinidad, Venezuela. Nicaragua, siempre acostumbran la sombra; y sin ir muy lejos, en el Valle del Cauca, donde no se cultiva a pleno sol y se obtienen plantaciones de larga duración y producto superior, es bien conocido el beneficio de ella; allí, los agricultores conocen los desastrosos efectos que ocasiona la falta de sombra, cuando por cualquier causa mueren los árboles que la suministran. Por tanto, en este estudio indicaremos el uso de la sombra para el cacao, como el medio de conservar la fertilidad y buenas condiciones del suelo, obtener buenos rendimientos y asegurar larga vida a la plantación.

  11. NOTAS SOBRE A IRRACIONALIDADE DA GUERRA

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    Edilene Leal

    2014-08-01

    Full Text Available Nesse artigo, procedemos a uma análise sobre a irracionalidade da guerra em tempos cada vez mais caracterizados pela evolução da racionalidade técnica, ou seja, em tempos contemporâneos. Esse é o ponto a partir do qual traçamos, aqui, um percurso argumentativo dividido em duas partes. Na primeira, reconstituímos elementos da concepção de guerra de filósofos como Maquiavel, Clausewitz e Kant, destacando, nos dois primeiros pensadores, a prerrogativa de que a guerra é racional quando se configura meio para fins políticos. Na segunda parte, confrontamos essa racionalidade política da guerra com a dupla dimensão técnica que a guerra assumiu: de um lado, a guerra como um fim em si mesmo e não mais como meio da ação política; de outro, a dimensão racionalizante da guerra como resultado do progresso técnico e científico, atualizada como guerra do terror.

  12. Cassirer e Sartre sobre o esclarecimento

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    Vinicius de Figueiredo

    2005-12-01

    Full Text Available O presente ensaio levanta alguns aspectos da estética no século XVIII. Partindo da apresentação de duas interpretações hoje clássicas sobre o assunto (a de Cassirer e a de Sartre, busca rever o pressuposto analítico comum a ambas, para, em seguida, apontar as conseqüências que a relação entre autor e público trazem para a compreensão de aspectos estilísticos que nos parecem centrais para a literatura do Esclarecimento.This paper aims at throwing some light on the Eighteenth-Century aesthetics. After examining two classical interpretations - Cassirer e Sartre's - concerning this subject, I argue that both authors share a common analytical pressupposition. My main purpose is to show that without taking account of the the relationship between author and public, we cannot understand some essential characteristics of the literature in the Enlightenment.

  13. Fechas y sospechas sobre Lucas de Tuy

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    Linehan, Peter

    2002-06-01

    Full Text Available The present essay seeks to establish what we know, as opposed to what we think we know, about the author of the Chronicon Mundi. Amongst the issues investigated are those of don Lucas's origins, the nature of his connections with León, the date of his activity as chronicler, and the use which may have been made of that work by D. Rodrigo Jiménez de Rada in the De rebus Hispaniae.

    El presente estudio busca establecer qué es lo que sabemos, en oposición a qué es lo que pensamos que sabemos, sobre el autor del Chronicon mundi. Entre los aspectos investigados están los orígenes de don Lucas de Tuy, la naturaleza de sus conexiones con León, la datación de su actividad como cronista así como el uso que pudiera haber hecho Rodrigo Jiménez de Rada de su obra en el De rebus Hispaniae.

  14. Consideraciones sobre diferentes procesos productivos y sus consecuencias sobre el medio ambiente

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    Caridad Ramos Alvariño

    2004-01-01

    Full Text Available En el presente trabajo se exponen algunas consideraciones sobre diferentes procesos productivos y sus consecuencias sobre el medio ambiente. La disminución del impacto negativo de algunas producciones sobre el medio ambiente es favorable, pero el problema va más allá y de lo que se trata es de la necesidad de una transformación sostenible de las producciones que puedan conducir a una vida agradable y una naturaleza conservada entre otras muchas cuestiones que agobian hoy al mundo. La solución al problema puede ser alcanzada con mayor prioridad en la reducci ón de la fuente primaria de la contaminación, seguida por el reciclaje y el desarrollo de tecnologías limpias. La reducción en la fuente se aplica por los cambios de productos y procesos. El cambio de la fuente se relaciona con la composición o el uso de los productos intermedios y finales, que reduzcan los residuos de producción, su uso o la disposición de los productos. Los cambios de procesos están relacionados con la sustitución de las materias primas de entrada, la modificación de las tecnologías y las mejoras operacionales. Las opciones específicas pueden incluir: el uso de materiales menos tóxicos, el aumento de la automatización, los procedimientos para las operaciones y el mantenimiento, el control de inventario, entre otros.

  15. Normativa sobre el impuesto sobre los gases efecto hivernadero (Green house gases tax)

    OpenAIRE

    Rodrigo de Larrucea, Jaime

    2013-01-01

    Como dice el apartado V de la Exposición de motivos de la Ley 16/2013, se crea un nuevo impuesto sobre los gases de efecto invernadero, relativo a los gases fluorados que se utilizan habitualmente en numerosos sectores como refrigerantes, disolventes, agentes espumantes o agentes extintores de incendios, para paliar de alguna manera su negativa contribución al calentamiento de la atmósfera, con un potencial de calentamiento global mucho más elevado que el CO2.

  16. Elevada porcentagem de blastocistose em escolares de São Paulo, SP Blastocystosis: a high proportion of cases found in schoolchildren of São Paulo, State of São Paulo, Brazil

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    Vicente Amato Neto

    2004-08-01

    Full Text Available Em exame parasitológico de fezes de 227 alunos de escola pública de São Paulo (SP, encontramos 87 (38,3% positivas para Blastocystis hominis. A blastocistose ainda suscita controvérsias e indefinições, merecedoras de esclarecimentos sobretudo para evitar contratempos no âmbito da saúde pública e das atenções médico-assistenciais.As a part of medical assistance activities, parasitological examination of fecal samples from 227 school children from a public institution of São Paulo (SP revealed a rather high proportion of results positive for Blastocystis hominis. Other protozoan and worm species were markedly scarcer, a peculiar situation according to our judgement. It is acknowledged that blastocystosis is still largely an indefinite and controversial subject, which deserves adequate analysis to avoid drawbacks in the sphere of action of public health and general medical assistance.

  17. Algunas reflexiones acerca de la encuesta sobre trabajo colaborativo

    OpenAIRE

    Gargiulo, Sandra; Ponz, María Jimena

    2014-01-01

    Comenzamos el mes de noviembre con un nuevo tema del mes: Dinámicas de trabajo colaborativo virtual. Para ello, queremos compartir los resultados arrojados por la encuesta sobre el trabajo colaborativo que muchos de ustedes han completado.

  18. Reflexiones sobre el género y la censura

    OpenAIRE

    Daniel Cazés Menache

    2006-01-01

    Este trabajo analiza y reflexiona sobre la primera censura social: la censura genérica, es decir, la estructurada sobre la adscripción de género, arena en la que se sintetizan todas las censuras sociales destinadas a controlar a cada sujeto y a mantenerlo sometido a dominios de los que no podrá librarse ni para aceptarlos ni para ejercerlos.

  19. Banco Interamericano de Desarrollo informe sobre sostenibilidad 2011

    OpenAIRE

    Banco Interamericano de Desarrollo (BID)

    2012-01-01

    El Informe de Sostenibilidad contiene información sobre las contribuciones del BID a la sostenibilidad durante el año 2011. También proporciona información sobre las tendencias fundamentales del desarrollo y las dimensiones de sostenibilidad en América Latina y el Caribe. Este año, el informe se centra en la sostenibilidad y la agricultura en la región

  20. Enteroparásitos: detección y vigilancia en aguas residuales de Costa Rica durante 1999

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    Mariano Peinador

    2000-12-01

    Full Text Available Durante el año 1999 se realizó un monitoreo de los cinco sistemas lagunares de tratamiento de aguas residuales domésticas y de una planta de Iodos activados que posee el Instituto Costarricense de Acueductos y Alcantarillados en Costa Rica. Este monitoreo consistió en toma y análisis de muestras bimensuales en el agua afluente y efluente de dichos sistemas de tratamiento. El objetivo de este trabajo es estudiar la eficiencia en la remoción de huevecillos de helmintos y quistes de protozoarios patógenos que tienen los diferentes sistemas de tratamiento y comparar los resultados con la epidemiología de las zonas correspondientes, al respecto. Las muestras se analizaron por el método de Ritchie modificado. Se encontró una baja eficiencia en la remoción de estos organismos por parte de la planta de Iodos activados, en lo que respecta a Entomoeba histolytica, E. Coli,Blastocystis hominis y Lamblia intestinalis, y una eficiencia del 100% en todos los sistemas lagunares. Estos resultados concuerdan con los datos sobre parasitosis por enteropatógenos, obtenidos de los diferentes hospitales y clínicas de las zonas en estudio.

  1. PROTOCOLO DE KYOTO: DEBATE SOBRE AMBIENTE Y DESARROLLO EN LAS DISCUSIONES SOBRE CAMBIO CLIMÁTICO

    Directory of Open Access Journals (Sweden)

    Rodríguez S. Liliana

    2008-07-01

    Full Text Available El comportamiento climático del planeta y sus consecuencias han propiciado debates sobre los modelos de desarrollo de los países responsables del deterioro acelerado del ambiente y de los fenómenos naturales por estos días recurrentes. Sin embargo, países como Estados Unidos, luego de firmar compromisos como la Convención sobre Cambio Climático, rehúsan adquirir las obligaciones del Protocolo de Kyoto, por temor a sufrir deterioro en sus economías. En este escenario, donde la nación responsable de la emisión de aproximadamente 36% de los gases efecto invernadero resultado de la acción humana en el planeta no se compromete a adoptar políticas restrictivas tendientes a hacer sus modelos de producción más amigables con la naturaleza a pesar de que dichas medidas empiezan a ser impuestas a otras naciones como condicionantes en las negociaciones comerciales internacionales, parece ser que la adopción del Protocolo de Kyoto para países en desarrollo como Colombia no es del todo benéfica.

  2. EFEITO DE CALDAS SOBRE Corynespora cassiicola

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    Mercia Celoto

    2014-11-01

    Full Text Available The target spot (Corynespora cassiicola, main leaf disease occurred in barbados cherry in the regionof Junqueirópolis, SP, causing severe defoliates of plants. After the cleaning prune, producers apply line sulfhur in plants, for presenting fungicidal, insecticide and acaricide action. The objective of this study was to assess the effect of line sulfhur (75% and 3% - used by producers, bordeaux mixture (75% and „calda Viçosa‟ (75% on Corynespora cassiicola. The syrups were incorporate into potato-dextrose-agar or spore suspension for determination of inhibition of the mycelia growth and spores germination. Leaves barbados cherry, with symptoms of target spot, were treated with syrups. In wet champer, high humidity, was verified that sporulation occured in lesions, however the spores lost viability by presence of syrups in surface. In vitro, line sulfhur to 75% inhibited completely the mycelia growth of the fungus. Line sulfhur, Bordeaux mixture and „calda Viçosa‟ inhibited completely the spores germination. For the reasons, the use of syrups in barbados cherry, can contribute in reduction of souce of inoculum of the pathogen. A mancha alvo (Corynespora cassiicola é a principal doença da cultura da acerola, na região de Junqueirópolis, SP, causando desfolha precoce nas plantas. Após a realização da poda de limpeza anual, os produtores aplicam a calda sulfocálcica nas plantas, por apresentar ação fungicida, inseticida e acaricida. O objetivo deste trabalho foi avaliar em condições in vitro e in vivo o efeito das caldas sulfocálcica, bordalesa e Viçosa sobre Corynespora cassiicola. As caldas foram utilizadas nas seguintes concentrações: calda sulfocálcica (75 e 3% - concentração utilizada por produtores, calda Bordalesa (75% e calda Viçosa (75%. As caldas foram incorporadas em meio de batata-dextrose-ágar ou suspensão de esporos para determinação das inibições do crescimento micelial e da germinação de esporos

  3. Sobre o campo da Psicologia Social

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    Belinda Mandelbaum

    2012-03-01

    Full Text Available O campo da Psicologia Social é apresentado como território fértil, na contemporaneidade, para constituir-se como um laboratório para a produção em Ciências Humanas, uma vez que, no século XX, cada vez mais o social foi em direção ao psicológico. Neste campo, nosso embate dá-se no modo como entendemos o hífen pressuposto na integração psicossocial. A autora propõe que, entre o psicológico e o social, o hífen domina, pois ele é a própria essência relacional que é inerente a cada um dos elementos. Sugere que o modelo para entender o homem e suas circunstâncias proposto por Freud imbrica de forma indissociável o psicológico e o social, a ontogênese e a filogênese, com uma potência que teve impacto sobre todo o campo das Ciências Humanas. A Psicanálise é um instrumento hermenêutico para colaborar na elucidação dos fenômenos sociais. A autora utiliza imagens construídas por Freud e Walter Benjamin e poemas de Carlos Drummond de Andrade para fortalecer o entendimento do hífen psicossocial tanto em sua ação multidimensional quanto em sua organização.

  4. Construcciones en yeso proyectado sobre vegetales

    Directory of Open Access Journals (Sweden)

    Martínez, Hubert

    1986-07-01

    Full Text Available A construction method developed in early 1981 in Senegal, whose main objective is the evaluation of natural and human sources, specially those of wild vegetables and the phosphogypsum (a waste of chemical industry in Senegal. The introduction of the mechanical projection allows a noticeably growth in work performances and an improvement of the quality. The projection reduces de number of trades intervening in the work, doing away with plasters, watertightness systems and paintings, and simplifying the setting of technical installations and of carpentry. The acquisition of this projecting material is not very expensive, so, it is easy for small and medium enterprises to reach an expertness in gypsum work. Its finantial rentability is assured for a prize of the gypsum of 180% of the prize of the cement.Procedimiento de construcción desarrollado al comienzo de 1981 en el Senegal, teniendo como objetivo la valoración de las fuentes naturales y humanas, especialmente los vegetales en estado silvestre y el fosfoyeso, residuo de la industria química del Senegal. La introducción de la proyección mecánica permite aumentar sensiblemente el rendimiento del trabajo y mejorar la calidad. La proyección reduce el número de oficios que intervienen en la obra por la supresión de enlucidos, sistemas de estanquidad y pinturas sobre la gruesa obra y simplifica la colocación de las instalaciones técnicas y de carpintería. La adquisición del material de proyección, necesitando un capital relativamente modesto, se encuentra al alcance de pequeñas y medianas empresas que deseen especializarse en los trabajos de yeso. La rentabilidad financiera del procedimiento está asegurada para un precio del yeso de hasta el 180% del precio del cemento.

  5. Puente sobre el Rhin – Suiza

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    Bänziger, D. J.

    1972-10-01

    Full Text Available In the brief period of two months a new bridge over the Rhine has been constructed to substitute one which collapsed on August 1970, because of the undermining of one of its pillars. On November 28 1970 the new bridge was opened to the traffic, including trucks of up to 3.5 Kp, and on December 7th of the same year vehicles of up to 26 Kp could use it. In the construction of this bridge, for the first time, use has been made of cylindrical, prefabricated, precast thin shells. This project is of special significance because of its operational importance, since it connects Lichtenstein and Switzerland; the exceptionally short time of its construction; and the new type of deck used.Para sustituir un puente sobre el Rhin, hundido el 14 de agosto de 1970 por socavación de uno de sus pilares, se ha construido un nuevo puente en el escaso plazo de dos meses. El 28 de noviembre de 1970, este nuevo puente se abrió al tráfico de vehículos de hasta 3,5 Mp, y el 7 de diciembre del mismo año, a los vehículos de hasta 26 Mp. En la construcción de este puente se han utilizado, por vez primera, láminas cilíndricas de cubierta, prefabricadas y pretensadas. La importancia del puente, que sirve para el enlace entre el Principado de Liechtenstein y Suiza, el nuevo tipo de tablero utilizado, así como el plazo de construcción, extraordinariamente breve, dan un interés especial a esta obra.

  6. Perspectivas sobre la comunicación

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    María Luisa Naranjo Pereira

    2005-01-01

    Full Text Available Existen diversas teorías, enfoques y modelos de comunicación; de cada uno de ellos pueden rescatarse aspectos de gran valor para el logro de una comunicación eficaz. Este documento presenta una revisión de esos aspectos, para que se pueda comprender mejor y enriquecer uno de los procesos más importantes para el ser humano: el proceso de comunicación. Este es un ensayo en el cual se da a conocer la posición de la autora sobre el tema abordado. Se rescata el acento del enfoque humanista en la relación humana y en las etapas donde se desarrolla. A partir de la comunicación pragmática, se analizan los axiomas comunicacionales en que se fundamenta y lo que se conoce como patologías de esos axiomas. Además se revisan las principales características que distinguen la asertividad en la comunicación y las distintas formas de manipulación que la entorpecen. Del enfoque de Virginia Satir, se presentan los diversos aspectos de la comunicación, tanto funcional como disfuncional y los modelos de comunicación propuestos por esta autora. Se revisan aspectos importantes del enfoque de la comunicación educativa, tales como el control de la distancia, la voz y el cuerpo, las palabras y el situacional; así como los elementos que bloquean el proceso de comunicación y las sugerencias acerca de formas correctas de escuchar. Además, se hace referencia a la necesidad de emplear procesos educativos más holísticos y constructivistas y una comunicación-interrelación horizontal y con equidad.

  7. Sobre el genio de las mujeres

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    Valcárcel, Amelia

    1992-11-01

    Full Text Available Not available.

    A los varones, desde Tácito a Schopenhauer, nunca les ha parecido que las verdaderas mujeres son las que tienen en casa, sino las que tienen los otros varones (los germanos, los orientales. Por su parte, Wojtyla ha hecho profesión de fe «en el genio de las mujeres», reclutando enternecidos agradecimientos como el de Macciochi. La autora del presente artículo adopta una perspectiva histórica y una actitud analítica para aclarar qué es ese genio que como genérico nos adjudican. En primer lugar, la idea de genio es romántica y va emparejada con la de destino, sobre todo en el caso de los genios colectivos. Se trata de nociones que se construyen estipulativamente con fines pragmáticos y no admiten, por definición, contraejemplos. En el caso del genio de las mujeres, los llamados «valores femeninos» que lo constituyen pertenecen al ámbito de la Siulichkeit, y su eventual convalidación en el de la Moralitiit no puede obviar el proceso consistente en la crítica y el desmarcarse de una Sittlichkeit adjudicada como estereotipia que se contrasta, no con otra estereotipia simétrica, sino con lo universal, que se solapa con el genérico masculino. La autora, por último, nos recuerda que la fabricación de este genio que, a título de colectivo, se nos atribuye a las mujeres tiene lugar a costa de la usurpación de nuestra individualidad y de la ausencia de esquemas pragmáticos de reconocimiento de la genialidad individual que pueda producirse en el genérico femenino. Ahora bien: es esta última la que las mujeres necesitamos para poder romper con las tradiciones establecidas e instituir nuevos relatos.

  8. Estudio sobre encofrados de madera modernos

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    de la Peña Aznar, Juan M.

    1980-12-01

    Full Text Available The author continues the development of the subject «Modern Timber Formwork», by summing up the comparative examination —already carried out in Part III of this Study— of the different types of glued timber existing on the market. In addition, the loads and stresses allovk/able for coniferous timber and the proposal for establishing Regulations for the Timber Section of the Research and Experimental Forestry Institute of Spain are given. In part V of the author's Study, published in this article, the important subject of the glues used for joining timber, a truly vital point in order to obtain louvered timber beams which are simply glued together, is approached.

    El autor continúa el desarrollo del tema sobre «Encofrados de madera modernos», resumiendo el estudio comparativo —ya hecho en la Parte III de este Estudio— de las diferentes vigas de madera encolada existentes en el mercado dando, además, las cargas y tensiones admisibles para maderas coníferas y la propuesta de Reglamentación de la Sección de Maderas del Instituto Forestal de Investigaciones y Experiencias de España. En la parte V del Estudio del autor, publicada en este artículo, se aborda el importante tema de las colas empleadas para las uniones de madera, algo realmente vital para la obtención de vigas de madera en celosía simplemente encoladas.

  9. Estudos sobre a Esquistosomose em Pernambuco, Brasil

    Directory of Open Access Journals (Sweden)

    Aggeu Magalhães

    1940-01-01

    Full Text Available Os autores referem os resultados dos estudos sobre esquistosomose realisados, em Pernambuco, de Novembro de 1938 a Dezembro de 1939. Não se extendem em discussão, nem comentarios, tampouco, relacionam suas verificações com o que já foi relatado sobre o assunto em trabalhos nacionais e estrangeiros, pelo fato de que, não se trata de um resultado definitivo e sim de atividades que irão proseguir. Os estudos sistematicos em torno da endemia esquistosomica foram executados em duas localidades diversas pela população e pela situação geografica e ecologica. Pontezinha é uma povoação de 1200 habitantes localizada proximo a um conjunto de charcos e lagõas de agua dôce onde pululam caramujos do tipo olivaceus de Spix, e apresenta uma taxa de infestação de 21,4% para individuos do sexo masculino, e de 14,7% para os do sexo feminino. A distribuição por grupos de idade mostra que a incidencia cresce até o grupo de 16 a 20 anos de idade, quando atinge a percentagem de 37,8%, para decrescer em seguida. Em Vitoria a incidencia foi maior nos indivíduos do sexo feminino, atingindo a taxa de 41,4%, dando o sexo masculino a taxa de 36,9%. Por idade a percentagem maxima é atingida pelo grupo de 11 a 15 anos. Vitoria é uma cidade de 1500 habitantes, construida á margem do rio Tapacurá, onde se encontram numerosos caramujos do tipo centimetralis Lutz. A divergencia de incidencia corre por conta dos habitos da população e pela utilização que ela faz do rio, sendo maior em Vitoria porque a proximidade do rio facilita o uso de suas aguas para banho e serviços domesticos, condicionando este ultimo fato, a maior infestação das mulheres. O estudo da frequencia da infestação dos caramujos por cercarias de diversos trematodios, permite suspeitar uma relação inversa entre a taxa de infestação e o diametro maximo atingido pelos caramujos, parecendo este fato confirmar os trabalhos de Vianna Martins sobre a identidade dos hospedeiros

  10. Methods for cultivation of luminal parasitic protists of clinical importance.

    Science.gov (United States)

    Clark, C Graham; Diamond, Louis S

    2002-07-01

    Cultivation of luminal protistan parasites has a long history. In this review we discuss the methods and media that are most widely used for the establishment and maintenance of the following organisms in culture: Entamoeba histolytica, Giardia intestinalis, Trichomonas vaginalis, Dientamoeba fragilis, Blastocystis hominis, and Balantidium coli. While cultivation is of limited importance in the diagnostic laboratory, it is essential to most research laboratories, and it is toward the latter that this review is primarily aimed. PMID:12097242

  11. Intestinal parasitoses and environmental factors in a rural population of Argentina, 2002-2003 Parasitosis intestinales y factores ambientales en una población rural de Argentina, 2002-2003

    OpenAIRE

    Juan A. Basualdo; Córdoba, María A; de Luca, María M; María L. Ciarmela; Betina C. Pezzani; María S. Grenovero; Marta C Minvielle

    2007-01-01

    We evaluate the prevalence of intestinal parasites in 504 people and the degree of association between environmental variables and parasites found in population, soil and water in a rural area of Argentina during 2002-2003. A structured survey was used to evaluate the environmental variables and fecal-human, soil and water samples were analyzed. The prevalence of parasites was 45.4%. Most prevalent protozoa were Blastocystis hominis (27.2%) and Giardia lamblia (6.9%), while the most prevalent...

  12. Surveillance for parasites in unaccompanied minor refugees migrating to Germany in 2015

    OpenAIRE

    Heudorf, Ursel; Karathana, Maria; Krackhardt, Bernhard; Huber, Meike; Raupp, Peter; Zinn, Christian

    2016-01-01

    In 2015, most of the refugees arriving in Germany originated from countries with poor hygienic and sanitary conditions. Stool samples of 1,230 minor refugees unaccompanied by adults were investigated for possible parasites. Giardia lamblia was by far the most frequently detected parasite (n=165); all other parasites were considerably less frequent and encountered in the following order: Hymenolepis nana (n=23), Entamoeba histolytica (n=17), Trichuris trichiura (n=8), and Blastocystis hominis ...

  13. Dois estudos sobre o trabalho dos petroleiros

    Directory of Open Access Journals (Sweden)

    Leda Leal Ferreira

    1996-06-01

    Full Text Available O presente artigo traz um resumo de dois estudos realizados na área do petróleo, o primeiro feito em uma unidade de refino e o segundo abarcando o trabalho de operadores exercendo várias funções dentro de uma refinaria e de terminais marítimos de petróleo. Nos dois casos, o ponto central foi a análise da atividade dos trabalhadores, isto é, o que eles faziam e como o faziam para dai conta das exigências de produção que lhes eram atribuídas. No entanto, os métodos utilizados foram diferentes: no primeiro caso utilizou-se a Análise Ergonômica do Trabalho (AET e no segundo, a Análise Coletiva do Trabalho (ACT. Além de apresentar os principais resultados destes estudos, a autora faz considerações sobre as diferenças entre os dois métodos e um apelo à comunidade científica para que se preocupe mais em estudar a atividade real dos trabalhadores e assim contribuir para um melhor conhecimento do que se passa no interior das unidades produtivas, evitando que sofrimentos humanos inúteis e até catástrofes industriais continuem a ocorrer.This paper describes two studies about petroleum industry. The first was developed in a refinery and the second focuses on the work of operators in various jobs within a refinery and maritime petroleum units. In both cases, the focal point was the analyses of activities, that is, what the workers do and how they do it in order to meet production demandai put on them. However, two different methods were applied in the studies. Ergonomics Work Analyses (EWA was used on the first case and Collective Work Analyses (CWA, on the second. Besides showing the main results, the author compares the two methods. She also draws the scientific community attention to the importance of studding the workers actual activity in order to contribute to a better understanding of what is going on inside production units, avoiding useless human suffering, and the still ocurring undustrial disasters.

  14. Marginalia: algumas notas adicionais sobre o dom

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    Pierre Bourdieu

    1996-10-01

    Full Text Available Prosseguindo a análise do dom, este artigo busca ultrapassar as visões fenomenológica, estruturalista e economicista do fenômeno. Para tanto, desloca o eixo de interrogação na direção do tempo e da incerteza ligados ao intervalo entre o dom e o contradom, do agente e da ação ligados às práticas, da economia de bens simbólicos e o habitus específico que a funda. Demonstrase assim, contra todo reducionismo (à consciência, ao cálculo inconsciente ou ao cálculo propriamente dito, que a razão do dom só pode repousar sobre sua dupla verdade, seu caráter generoso e obrigatório. Por outro lado, demonstrase também que foi somente através de uma revolução simbólica, e não o desenvolvimento de uma suposta natureza humana, que uma economia da troca pôde, historicamente, se destacar da economia do dom, cuja possibilidade continua aberta, dependendo apenas dos investimentos coletivos que sejam efetuados em sua direção.Resuming an analysis of the gift, this article seeks to go beyond the phenomenological, structuralist, and economicist view of this phenomenon. It thereby shifts the trust of questioning towards the time and uncertainty linked with the interval between the gift and the counter-gift, the agent and the action linked to such practices, the economy of symbolic goods, and the specific habitus underlying it. It thus demonstrates against any and all reductionism (to consciousness, to unconcious calculation, and to calculation per se that the gift's reason can only lie in its double truth, its generous and obligatory nature, both disinterested and interested. The author also demonstrates historically that it is only through a symbolic revolution, and not the development of a supposed human nature, that an economy of exchange can stand out from the economy of the gift, whose possibility remains open, requiring collective investments in its direction.

  15. As vicissitudes do "ensaio sobre o dom"

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    Lygia Sigaud

    1999-10-01

    Full Text Available A partir dos anos 60, antropólogos de diferentes tradições nacionais passaram a convergir no sentido de considerar que o texto de Marcel Mauss, "Ensaio sobre o Dom", contivesse uma teoria da troca, que atribui à identificação entre a coisa dada e o espírito do doador o princípio de explicação das transações. Esta interpretação contrasta com a que tiveram os contemporâneos de Mauss, os quais resgataram do trabalho suas dimensões relativas ao direito, às obrigações e às prestações totais. Partindo desta constatação, o artigo problematiza o destino do ensaio e busca torná-lo inteligível. Para tanto examina as condições sociointelectuais que contribuíram para que se produzisse a inflexão na leitura e a consolidação de uma espécie de crença coletiva em relação ao trabalho. Através deste estudo de caso, a autora identifica mecanismos que operam no processo de construção de teorias, na conformação de representações acerca de textos e na sua consagração no âmbito da antropologia.From the 1960s, anthropologists of different national traditions came to share the view that Marcel Mauss’s text, "Essay on the Gift," contained a theory of exchange which takes the identification between the thing given and the spirit of the donor as the explanatory principle of transactions. This interpretation contrasts with that held by Mauss’s contemporaries, who lifted from the work aspects relating to law, obligations and total prestations. Starting from this fact, the article problematizes the essay’s fate and seeks to make it intelligible. To this end, it examines the socio-intellectual conditions which contributed to producing this inflection in the readings of the work and the consolidation of a kind of collective belief concerning it. Through this case study, the author identifies mechanisms which operate in the process of constructing theories, in the conformity found in representations of certain texts, and in the

  16. Nota sobre Marcel Mauss e o ensaio sobre a dádiva

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    Lanna Marcos

    2000-01-01

    Full Text Available Este artigo analisa a obra clássica de M. Mauss, Ensaio sobre a dádiva, à luz de desenvolvimentos recentes da Antropologia. Salienta como contribuição de Mauss o entendimento da dimensão política da troca de dádivas, assim como a sugestão de sua universalidade, posteriormente demonstrada por Lévi-Strauss, constituir-se em princípio formal-abstrato, e não num fato empírico-concreto. A partir desse princípio, avalia a tese segundo a qual a dádiva é fundamento de toda sociabilidade e comunicação humanas, assim como sua presença e sua diferente institucionalização em várias sociedades analisadas por Mauss, capitalistas e não-capitalistas.

  17. Comentarios sobre la Ley de Ordenamiento Territorial Sostenible

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    Arturo J. Yglesias

    2014-04-01

    Full Text Available La denominada Ley de Ordenamiento Territorial Sostenible, no fue bien recibida en el medio jurídico. Tanto los profesores de las diferentes materias de Derecho de nuestra Facultad como las asociaciones profesionales y otros grupos e juristas de nuestro medio, formularon reservas de diverso calibre sobre el texto de la ley que van desde sus aspectos adjetivos como la terminología y redacción hasta cuestiones sustanciales como lo que ella dice sobre el régimen de la propiedad territorial y otras cuestiones vinculadas.Desde el discurso político vimos lanzar opiniones iracundas y alarmistas sobre las modificaciones que la ley estaría haciendo al régimen de la propiedad privada, sobre su supuesta inconstitucionalidad.Nosotros nos vamos a referir a la ley a sus antecedentes y de las críticas que se le hacían, en la parte que toca a nuestra materia, esto es en lo relativo al régimen de la propiedad y debemos decir, a vía de adelanto, que no vemos en ella nada que afecte el derecho de propiedad consagrado por la Constitución de la República, ni los principios generales que regulan la propiedad territorial conforme al Código Civil, conforme a la vieja ley de Centros Poblados, sus modificativas ni demás normas sobre la materia.

  18. Perspectivas sobre la comunicación

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    Naranjo Pereira, María Luisa

    2005-12-01

    Full Text Available Existen diversas teorías, enfoques y modelos de comunicación; de cada uno de ellos pueden rescatarse aspectos de gran valor para el logro de una comunicación eficaz. Este documento presenta una revisión de esos aspectos, para que se pueda comprender mejor y enriquecer uno de los procesos más importantes para el ser humano: el proceso de comunicación. Este es un ensayo en el cual se da a conocer la posición de la autora sobre el tema abordado. Se rescata el acento del enfoque humanista en la relación humana y en las etapas donde se desarrolla. A partir de la comunicación pragmática, se analizan los axiomas comunicacionales en que se fundamenta y lo que se conoce como patologías de esos axiomas. Además se revisan las principales características que distinguen la asertividad en la comunicación y las distintas formas de manipulación que la entorpecen. Del enfoque de Virginia Satir, se presentan los diversos aspectos de la comunicación, tanto funcional como disfuncional y los modelos de comunicación propuestos por esta autora. Se revisan aspectos importantes del enfoque de la comunicación educativa, tales como el control de la distancia, la voz y el cuerpo, las palabras y el situacional; así como los elementos que bloquean el proceso de comunicación y las sugerencias acerca de formas correctas de escuchar. Además, se hace referencia a la necesidad de emplear procesos educativos más holísticos y constructivistas y una comunicación-interrelación horizontal y con equidad. Each of the diverse theories, approaches, and models of communication can provide valuable elements for the attainment of effective communication. This article presents a review of those elements with the goal of providing a better understanding and enhancement of one of the most important processes for human beings: the process of communication. This essay presents the author´s position on the topic. The importance given to human relationships and the stages

  19. Efeitos dos choques fiscais sobre o mercado de trabalho brasileiro

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    Diogo Baerlocher Carvalho

    2013-06-01

    Full Text Available Esse trabalho tem por objetivo entender quais os efeitos dos choques fiscais sobre o mercado de trabalho brasileiro. Em particular, utiliza se uma abordagem SVAR para avaliar esses efeitos sobre o emprego e o salário real. Os choques fiscais são identificados a partir da imposição de restrições de sinais puras sobre a relação dinâmica entre os gastos do governo, o produto real e a oferta de trabalho. As restrições são obtidas a partir de funções de resposta a impulso Bayesianas derivadas de um modelo DSGE na tradição da Nova Síntese Neoclássica. As funções de resposta a impulso obtidas a partir da abordagem SVAR mostram que choques fiscais aumentam o salário real e o emprego.

  20. Revisitando uma carta em português sobre Sabbatai Zvi

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    Rodrigues da Silva Tavim, José Alberto

    2007-06-01

    Full Text Available The present paper deals with the Iberian context of the production-translation of a text in Portuguese language about Shabbatai Zevi, in 1666. We refer the production of similar texts and the information about the adherents in Spain, mostly in Malaga. We reflect on the various messsianic currents that crossed the different social groups in Portugal. We present a text that proves the spreading of news about Shabbatai in Portuguese Lands, and the interest kindled among the Portuguese Conversos. And we place the hypothesis that this text to the movement of Shabbatai, could have different readings, among them one that could be convenient to the conversos.

    Este artículo aborda el contexto de la producción-traducción de un texto en portugués sobre Sabetay Sebí, en 1666. Se alude a la producción de otros textos similares y a la información sobre los adeptos del movimiento en España, sobre todo en Málaga. Se reflexiona sobre las varias corrrientes mesiánicas que cruzaban los diferentes grupos sociales en Portugal. Se presenta un texto que prueba la divulgación de noticias sobre Sabetay, en tierras portuguesas y del interés desperttado entre los medios conversos sobre el movimiento. Y se plantea la hipótesis de que este texto contrario al movimiento de Sabbatai pudiera tener varias lecturas, entre ellas una que debería ser conveniente para los conversos.

  1. Santa sexualidad: concepciones de dos monjas sobre feminidad y sexualidad

    OpenAIRE

    Isabel Cristina Giraldo Quijano

    2009-01-01

    En el contexto de la religión católica, la feminidad y la sexualidad femenina se configuran según dogmas y representaciones sociales específicos, en los que la imagen de Cristo -y todo lo que ello implica- actúa como elemento fundamental en la construcción de las concepciones de las mujeres consagradas a la vida religiosa sobre sex ualidad, erotismo, cuerpo. amor. Este artículo pretende explorar desde un punto de vista sociológico las concepciones de dos monjas sobre los temas mencionados....

  2. Datos sobre la vida sexual en dos ciudades colombianas

    OpenAIRE

    Giraldo Neira, Octavio

    2012-01-01

    Uno de los temas más difíciles de investigar científicamente es el referente a la vida sexual, principalmente por la dificultad de obtener datos precisos y verídicos sobre la conducta de los individuos. Este es un tema que se trata vagamente ó solo a través de la consulta médica ó el chiste. Resulta difícil preguntar a la gente sobre su conducta sexual y más difícil aun obtener una respuesta verdadera y clara. Este informe, aunque breve, ha logrado en parte eludir estos escollos. En primer lu...

  3. Fiscalidad de las entidades financieras. Impuesto sobre Sociedades

    OpenAIRE

    Muñoz Ortega, Julián

    2015-01-01

    Las entidades de crédito experimentan cambios de calado en su tributación directa con la reforma 2014 del Impuesto sobre Sociedades, en especial por lo que respecta a la nueva regulación de las pérdidas por deterioro y la doble imposición nacional e internacional Archivo con presentación de las novedades más relevantes a aplicar por entidades de crédito a partir de la Ley 27/2014, de 27 de noviembre, del Impuesto sobre Sociedades

  4. Diálogos sobre juventudes : Estudios de otras miradas

    OpenAIRE

    Fornasari, María Elisa; Decca, Lorena

    2012-01-01

    Medina, Gabriel (ed.) (2009). Juventudes, territorios de identidad y tecnologías. Círculo de Reflexiones de Estudios Poscoloniales. Universidad Autónoma de la Ciudad de México. México. Los estudios sobre juventud/es han seguido diversas trayectorias y han sido abordados desde diferentes perspectivas a partir de su visibilización como problemática. El libro de Gabriel Medina parte de un diagnóstico: la existencia de importantes vacíos en los estudios sobre lo joven y la vigencia de trabajos...

  5. La perspectiva cualitativa en los estudios sobre pobreza

    OpenAIRE

    Mateo Pérez, Miguel A.

    2002-01-01

    Los estudios sobre pobreza internacional han estado marcados por el enfoque cuantitativo. Sin embargo desde hace unos años los estudios cualitativos están adquiriendo una gran relevancia. Este artículo presenta las características de un estudio cualitativo sobre pobreza y género realizado en España durante los últimos tres años, estableciendo algunas líneas de trabajo básicas para el estudio de la pobreza desde la perspectiva cualitativa.

  6. Códigos y grafos sobre anillos de enteros complejos

    OpenAIRE

    Martínez Fernández, María del Carmen

    2007-01-01

    RESUMEN: El objetivo de esta tesis es definir códigos perfectos sobre diferentes espacios de señal multidimensionales. Para resolver este problema, esta memoria presenta una relación original entre las Teorías de Grafos, Números y Códigos. Uno de nuestros principales resultados es la propuesta de una métrica adecuada sobre constelaciones de señal de tipo cuadrático, hexagonal y cuatro-dimensional. Esta métrica es la distancia entre los vértices de una nueva clase de grafos de Cayley definidos...

  7. Banco Interamericano de Desarrollo informe sobre sostenibilidad 2010

    OpenAIRE

    Banco Interamericano de Desarrollo (BID)

    2011-01-01

    Este es el sexto Informe Anual sobre Sostenibilidad del Banco. Cubre el progreso y desempeño del Banco Interamericano de Desarrollo en materia de sostenibilidad durante el año fiscal 2010. El documento proporciona información sobre las tendencias fundamentales del desarrollo y las dimensiones relacionadas con la sostenibilidad en América Latina y el Caribe. Estas tendencias afectan, tanto positiva como negativamente, la capacidad de los países para garantizar la sostenibilidad, el crecimiento...

  8. Concepciones implícitas sobre el desarrollo infantil

    OpenAIRE

    Vilar Martínez, C.; Blasco Cubedo, María Jesús; Hernández Blasi, Carlos

    2001-01-01

    Setenes Jornades de Foment de la Investigació de la FCHS (Any 2001-2002) Partiendo del trabajo de Martí, E. et al. (1994) “ Ideas previas de una población universitaria sobre el desarrollo infantil” hemos realizado un estudio descriptivo para identificar las concepciones implícitas del conocimiento que tienen estudiantes universitarios sobre el desarrollo infantil. La muestra estaba constituida por 111 estudiantes de la Universitat Jaume I, con una media de edad de 19 años. Median...

  9. La tributación estatal sobre los residuos nucleares

    OpenAIRE

    Rozas Valdés, José Andrés

    2014-01-01

    Este trabajo se dedica al análisis pormenorizado de los nuevos impuestos estatales -'Impuesto de la producción de combustible nuclear gastado y residuos radiactivos resultantes de la generación de energía nucleoeléctrica' y el 'Impuesto sobre el almacenamiento del combustible nuclear gestado y residuos radiactivos en instalaciones centralizadas'- a partir de sus fundamentos, para terminar con unas consideraciones sobre su compatibilidad con las tasas equivalentes y con los impuestos autonómic...

  10. UN PENSAMIENTO EMERGENTE SOBRE EL ARTE CONTEMPORÁNEO

    OpenAIRE

    Ingrid Solana

    2009-01-01

    Este artículo trata de configurar un pensamiento emergente en torno a la reflexividad actual sobre el arte. Para ello, se analizan el stencil y el net.art como medios expresivos eminentemente autocríticos. Este ensayo recoge algunos aspectos teóricos sobre el pensamiento actual en torno al arte. Se basa, en su mayor parte, en las teorías estéticas de Gilles Deleuze y de Félix Guattari en ¿Qué es la filosofía?, aunque señala múltiples problemáticas que conciernen al pensamiento posmoderno. Las...

  11. Drug sensitivity of ureaplasma unrealytium accompanying mycoplasma hominis infection in female genital tract and its therapeutic effect%女性生殖道解脲支原体合并人型支原体感染药敏分析及疗效观察

    Institute of Scientific and Technical Information of China (English)

    郭树琴; 王振国; 宋萍

    2011-01-01

    目的 评价联合用药与单独用药治疗女性生殖道感染的疗效.方法 将2009年10月-2010年10月我院妇科门诊就诊76例生殖道解脲支原体(Ureaplasma Urealyticum)合并人型支原体(Mycoplasma Hominis)感染患者随机分成2组.联合用药组:外阴阴道冲洗(0.9%氯化钠注射液稀释5%碘伏1:10),口服强力霉素每次4mg,1次/d,连服7d,同时干扰素栓剂置阴道深部,隔日1次,共14d.单独用药组:口服强力霉素每次4mg,1次/d,共7d.停药1周后行妇科检查,停药2周后做官颈支原体培养.结果 76例患者对强力霉素、交沙霉素、美满霉素较敏感,敏感率依次为61.9%、59.2%和50%,而对罗红霉素、螺旋霉素、克拉霉素高度耐药,耐药率依次为90.8%、88.2%和76.3%.联合用药组总有效率92.1%,单独用药组总有效率78.9%,有显著差异(P<0.001).结论 应用外用干扰素栓剂联合强力霉素比单独口服强力霉素治疗女性生殖道解脲支原体合并人型支原体感染效果好.%To evaluate the therapeutic effect of combined doxycycline and interferon suppository or doxycycline alone on female genital tract infection. Methods Seventy-six patients infected with ureaplasma unrealytium and mycoplasma hominis who visited our department from October 2009 to October 2010 were randomly divided into combined drug treatment group(group A) and single drug treatment group(group B). Vulvovagina of patients in group A was irrigated with 0.9% normal saline and 5% iodophor(l:10). Then, the patients were treated with doxycycline(4mg), once a day for 7 days. Interferon suppository was placed in the deep vagina, every other day for 14 days. Patients in group B were treated with oral doxycycline only(4mg), once a day for 7 days. One week after withdrawal of the drugs, the patients in two groups underwent gynecological examination. Two weeks after withdrawal of the drugs, mycoplasma from cervix was cultured. Results The sensitivity rate of the 76 patients to

  12. "Meu prazer agora é risco": sentidos sobre sexualidade entre jovens de um grupo sobre saúde

    OpenAIRE

    João Paulo Pereira Barros; Veriana de Fátima Rodrigues Colaço

    2013-01-01

    A sexualidade assumiu a condição de centralidade subjetiva pelo entrecruzamento de diversos demarcadores da Modernidade, como a legitimação do saber-verdade da ciência, a afirmação da individualidade e a institucionalização dos segmentos infanto-juvenis. Partindo dessa premissa, este artigo analisa sentidos produzidos por jovens sobre "sexualidade" em um grupo de discussão sobre saúde, com base em uma pesquisa-intervenção operacionalizada por observações-participantes e pela formação de ofici...

  13. Protocolo de kyoto: debate sobre ambiente y desarrollo en las discusiones sobre cambio climático

    OpenAIRE

    Rodríguez S. Liliana

    2008-01-01

    El comportamiento climático del planeta y sus consecuencias han propiciado debates sobre los modelos de desarrollo de los países responsables del deterioro acelerado del ambiente y de los fenómenos naturales por estos días recurrentes. Sin embargo, países como Estados Unidos, luego de firmar compromisos como la Convención sobre Cambio Climático, rehúsan adquirir las obligaciones del Protocolo de Kyoto, por temor a sufrir deterioro en sus economías. En este escenario, donde la nació...

  14. Controversia sobre el origen del anime. Una nueva perspectiva sobre el primer dibujo animado japonés

    OpenAIRE

    Horno López, Antonio

    2012-01-01

    Recientes descubrimientos sobre los orígenes de la animación japonesa muestran indicios consistentes de que la primera animación nipona en formato cinematográfico data de la década de 1900, lo que puede llegar a cuestionar algunos aspectos fundamentales que sustentan la historia de la animación tradicional. En este artículo se realiza un análisis de la documentación existente sobre las primeras animaciones japonesas, demostrando que este peculiar estilo de animación tuvo en realidad un origen...

  15. Prevalence of Cryptosporidium spp. and other intestinal parasites in children with diarrhea

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    Mutalip Çiçek

    2011-03-01

    Full Text Available This study was planned to determine the role of Cryptosporidium sp. and other intestinal parasites in the diarrheal diseases in children with 0-15 years old Van district.Materials and methods: In this study, stool samples of 450 children were examined for parasites. In the study, nativ-lugol, formaldehyde-ethyl acetate sedimentation methods and trichrome staining methods were used to detect parasites in stool samples. Additionally, sedimentation methods and modified acid fast staining method were used to detect the Cryptosporidium oocysts.Results: Parasites were found in 154 (34.2% among 450 children’s with diarrhea. In this study; the ratios of parasites were as follow: Giardia intestinalis 13.5%, Blastocystis hominis 10%, Entamoeba coli 3.78%, Cryptosporidium spp. 2.2%, Hymenolepis nana 1.33 %ve Ascaris lumbricoides 1.11%.Entamoeba histolytica/Entamoeba dispar 0.89%, Chilomastix mesnili 1.78%, Iodamoeba butschlii 0.89%, Entamoeba hartmanni 0.89%, Trichomonas hominis 0.67%, Enteromonas hominis 0.67%,Conclusion: In the investigate, it was found that Giardia intestinalis and Blastocystis hominis were most prominent agents in children with diarrhea in our vicinity and Cryptosporidium spp also was an important agent which should be investigated carefully in especially risk group in routine laboratory studies.

  16. Construcciones sociales sobre mujeres desde el discurso jurídico en sentencias penales sobre infanticidio

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    María Eugenia Gastiazoro

    2015-12-01

    Full Text Available El infanticidio como figura penal se suprime del Código Penal Argentino en 1994, para ser una figura de homicidio agravado por el vínculo con prisión o reclusión perpetua. Diez años después de su última derogación, el caso Romina Tejerina en Jujuy generó una serie de cuestionamientos respecto de su penalización. Otro caso, que tomó estado público fue el de Eli Díaz en la ciudad de Villa Dolores en Córdoba, juzgada en el 2006, siendo (en contraposición al caso Tejerina absuelta por una mayoría compuesta solo de ciudadanos comunes (jurados. En el presente trabajo se analiza la construcción y la producción que desde el discurso jurídico se hace de las diferentes mujeres en los casos de infanticidio. A su vez, estas imágenes se comparan con las representaciones que los legisladores tuvieron en el debate del Congreso del año 2010 cuando se intentó reponer la figura. La sujeción del género centrada en la buena o mala madre, mujer, esposa, en intersección con otras dimensiones de clase social, y edad, se categoriza en el discurso de los tribunales de acuerdo a imaginarios sociales que sostienen una identidad normativa sobre estas mujeres.

  17. Sobre-educación en el mercado laboral colombiano

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    Jhon James Mora

    2008-12-01

    Full Text Available Este artículo analiza la sobre-educación en Colombia con datos de individuos y empresas compilados por el Servicio Nacional de Aprendizaje de Colombia en 2006, encuentra que la probabilidad de estar sobre-educado es del 14% y que depende de la movilidad entre áreas de desempeño, la experiencia, el género, el tamaño de la empresa y el nivel de educación. Los trabajadores sobre-educados ganan un 2% menos que los trabajadores correctamente educados, conforme al patrón internacional. Los resultados también muestran que la probabilidad de que los profesionales permanezcan en la misma área de desempeño aumenta con la experiencia, que cuanto mayor es la sobre-educación menor es la probabilidad de permanecer en dicha área y que los trabajadores con postgrado tienen menor probabilidad de permanecer en su área de desempeño.

  18. Reflexiones sobre la dirección de centros

    OpenAIRE

    Felpeto, Ángel

    2004-01-01

    Se presenta una reflexión sobre el modelo de dirección de centros desde la experiencia en la gestión de dos centros educativos de muy diferente tamaño y en diferentes épocas. El autor plantea la necesidad de un director líder, que encabece un proyecto educativo gestionado por un equipo.

  19. Sobre el costo de los protocolos de commitment

    OpenAIRE

    Zanconi, Marcelo; Ardenghi, Jorge Raúl

    1995-01-01

    Se presenta en este trabajo, una evaluación sobre los costos de los protocolos de terminación en dos fases, relacionados con la correctitud y la serializabilidad, considerando los mecanismos de apropiación de recursos basados en locking, time-sta.mping y control optimista.

  20. SONETO SOBRE O MISTÉRIO DO AMOR

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    Jorge Antunes

    2012-01-01

    Full Text Available SONETO SOBRE O MISTÉRIO DO AMOR Dedicado ao Prof. Dioney Moreira Gomes Jorge Antunes Um expert em linguística pariu dois meninos simpáticos, felizes. De um homem que era só então surgiu planta viçosa plena de raízes.

  1. Reflexiones y recomendaciones sobre la poda de frutales

    OpenAIRE

    Urbina Vallejo, Valero; Dalmases Mestre, Josep; Pascual Roca, Miquel

    1999-01-01

    Ante la nueva campaña de poda invernal se plantean una serie de consideraciones que pueden ser de utilidad para el fruticultor a la hora de tomar decisiones en la planificación de la poda y en la ejecución de las diferentes intervenciones que se llevan a cabo sobre el árbol.

  2. Aspectos sobresalientes del Informe sobre Sostenibilidad de 2012

    OpenAIRE

    Banco Interamericano de Desarrollo (BID)

    2013-01-01

    Este Informe sobre Sostenibilidad abarca el progreso y desempeño del Banco Interamericano de Desarrollo en materia de sostenibilidad durante el ejercicio fiscal de 2012. En este informe nos enfocamos en el tema emergente de la gestión de recursos hídricos.

  3. Apuntes de José Antonio Valverde sobre dientes

    OpenAIRE

    Valverde Gómez, José Antonio, 1926-2003

    2008-01-01

    Apuntes sobre dientes, que el autor utilizó para elaborar su conferencia "L'Evolution Humaine Apercue Generale", impartida en el Musèe de l'Homme de París, y patrocinada por la Société d’Anthropologie de Paris, el 21 de marzo de 1963.

  4. Apuntes de José Antonio Valverde sobre manos

    OpenAIRE

    Valverde Gómez, José Antonio, 1926-2003

    2008-01-01

    Apuntes sobre manos, que el autor utilizó para elaborar su conferencia "L'Evolution Humaine Apercue Generale", impartida en el Musèe de l'Homme de París, y patrocinada por la Société d’Anthropologie de Paris, el 21 de marzo de 1963.

  5. Las competencias sobre cooperativas y econom??a social

    OpenAIRE

    P??rez-Villalobos, M. Concepci??n

    2007-01-01

    El cap??tulo desarrolla la competencia sobre cooperativas y econom??a social de las Comunidades Aut??nomas espa??olas. El t??rmino "econom??a social" es nuevo en los Estatutos de autonom??a, aunque la constituci??n espa??ola apost?? fuerte por ??l como instituci??n que coopera al desarrollo del Estado social

  6. Sobre o estilo e o modo de falar e escrever

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    Pedro Caruso

    2001-02-01

    Full Text Available

    Este trabalho apresenta o texto crítico de uma Lei ou Provisão de Filipe I , rei de Portugal, sobre as diversas formas de tratamento no período arcaico da língua portuguesa.

  7. Integración de las actuales definiciones sobre función ejecutiva

    OpenAIRE

    Cadavid Ruiz, Natalia; Río Pereda, Pablo del; Martínez, R.

    2007-01-01

    Cartel expositivo del curso sobre Integración de las actuales definiciones sobre función ejecutiva realizado por la Universidad de Salamanca en colaboración con la Universidad Carlos III de Madrid.

  8. "Meu prazer agora é risco": sentidos sobre sexualidade entre jovens de um grupo sobre saúde

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    João Paulo Pereira Barros

    2013-04-01

    Full Text Available A sexualidade assumiu a condição de centralidade subjetiva pelo entrecruzamento de diversos demarcadores da Modernidade, como a legitimação do saber-verdade da ciência, a afirmação da individualidade e a institucionalização dos segmentos infanto-juvenis. Partindo dessa premissa, este artigo analisa sentidos produzidos por jovens sobre "sexualidade" em um grupo de discussão sobre saúde, com base em uma pesquisa-intervenção operacionalizada por observações-participantes e pela formação de oficinas sobre saúde, em uma escola pública de Fortaleza. Acerca dos sentidos sobre "sexualidade", as articulações entre "sexualidade" e "risco" se sobressaíram em relação às conexões entre "sexualidade" e "prazer". Isso sugere a força de um viés educativo baseado na scientia sexualis, aludido por Michael Foucault, operando com uma visão normativa-prescritiva da sexualidade, reiterando-a, mediante o signo da "prevenção", como tecnologia de governo. Usando como ferramenta o conceito de biopolítica, aponta-se que tais questões remetem à instalação de um paradigma de "gestão do risco" na contemporaneidade.

  9. EL PENSAMIENTO SOCIAL SOBRE EL SUICIDIO EN ESTUDIANTES DE BACHILLERATO

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    Gabriel Montes Sosa

    2009-01-01

    Full Text Available En este estudio se analiza desde el marco de las representaciones sociales cómo los jóvenes preparatorianos manifiestan su pensamiento social sobre el suicidio. Se aplicaron cuestionarios abiertos a 169 jóvenes, mujeres y varones de bachillerato, para conocer la ocurrencia potencial de suicidio, las situaciones a las que se debe y su definición. Los resultados muestran que los jóvenes organizan su idea sobre el suicidio a partir de varios elementos en común: problemas diversos, depresión, baja autoestima y comunicación deficiente. Así, el suicidio se objetiviza en estos esquemas y se ancla en sus relaciones existentes. Para contextualizar el estudio, en la primera parte del trabajo los autores incluyen una somera descripción de los datos estadísticos más importantes respecto del suicidio en el estado de Puebla.

  10. Preguntas y consideraciones sobre anestesia en el anciano

    OpenAIRE

    Evangelina Dávila Cabo de Villa

    2011-01-01

    El avance en los conocimientos sobre la fisiopatología de las enfermedades, apoyado por los adelantos científicos ha hecho posible la realización de mayor número de procedimientos quirúrgicos y con más complejidad, bajo la aplicación de métodos anestésicos y con calidad en los desempeños. El empleo juicioso de fármacos, la actualización tecnológica, la preocupación por crear mayores beneficios en los enfermos, conforman objetivos fundamentales en la anestesiología, sobre todo en ancianos. Es...

  11. Consenso Cultural sobre el Intento de Suicidio en Adolescentes

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    Javier Eduardo García de Alba García

    2011-01-01

    Full Text Available Este estudio buscó identificar la estructura semántica del dominio cultural, el promedio de conocimiento y el grado de consenso cultural manifestado por los adolescentes sobre el intento de suicidio. Fue un estudio cualitativo de listas libres, con un muestreo propositivo no aleatorizado de 27 adolescentes entre 13 y 18 años. Los resultados evidenciaron un solo modelo semántico. En lo estructural se identificó el problema familiar como una causa importante del intento suicida; las categorías depresión y tristeza se consideraron como signos y síntomas previos a esta tentativa y no como causas. Como estrategias de prevención, se consideró recibir información mediante pláticas o establecer conversaciones sobre los problemas. Este modelo permite proponer estrategias de prevención que privilegien el núcleo familiar.

  12. Passagens sobre o moderno na cidade de Georg Simmel

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    Felipe Ziotti Narita

    2013-07-01

    Full Text Available Este trabalho sugere alguns pontos para reflexão sobre o ensaio “As grandes cidades e a vida do espírito” (publicado em 1903, de Georg Simmel. Trata-se, fundamentalmente, de discutir as principais balizas teóricas e conceituais construídas pelo autor para refletir sobre a vida urbana, analisando, a partir das formas das interações sociais, as próprias especificidades da modernidade. Além do citado ensaio, este artigo discute as análises de Simmel à luz de alguns debates sociológicos de seu tempo, tomando como referência o conjunto mais amplo de seus trabalhos e interlocutores.

  13. Sobre a Pesquisa nas Artes: um discurso amoroso

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    Victoria Pérez Royo

    2015-09-01

    Full Text Available Este artigo resulta de um exercício de reescrita experimental e de um pensamento analógico cujo objetivo era abrir uma nova perspectiva sobre pesquisa nas artes. Metodologicamente, as diferentes figuras de amor no livro Fragmentos de um Discurso Amoroso, de Barthes, são levadas a conversar sobre a relação entre pesquisador e objeto de estudo. Essa analogia nos permite encontrar parâmetros de qualidade baseados em uma escala de valores diferentes dos hegemônicos na academia (produtividade, competitividade, inovação. Esses novos parâmetros podem constituir uma sólida base ontológica para a construção de uma nova política de pesquisa artística na academia que permita uma reconsideração radical dos processos de investigação em artes.

  14. Dialogos negativos anotaciones sobre el montaje de Medea Okupa

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    Marcelo José Islas

    2013-09-01

    Full Text Available En este texto hablamos de dos temas: la adaptación de una obra de Eurípides, ]Medea, y su montaje en el contexto de un espacio público a partir de un trabajo de investigación en actuación sobre la poética de la tragedia.Estos dos temas, que exponemos por separado una mejor comprensión.

  15. Sobre inundaciones y anegamientos / Reflections on floods and flooding

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    Ferrando A., Francisco J.

    2006-11-01

    Full Text Available Respecto a anegamientos e inundaciones, el autor realiza algunas precisiones conceptuales que afectan la gestión de acciones preventivas, la planificación y el ordenamiento territorial; además se ofrece una sistematización del quehacer sobre las inundaciones./ The author punctualizes the concepts regarding preventive actions and territorial planning. Also the article includes a systematized list of actions related to flood management.

  16. El buen vivir repolitiza los debates sobre el desarrollo

    OpenAIRE

    Gudynas, Eduardo

    2014-01-01

    El presente texto relaciona una importante entrevista realizado por Eduardo Gydynas, Investigador en el Centro Latino Americano de Ecología Social (CLAES), de Montevideo, Uruguay, al pensador Arturo Escobar. Se trató de un abierto diálogo donde el entrevistado, comenta algunas vertientes sobre las alternativas al desarrollo y el Buen Vivir. Encuentro personal, realizado en la Universidad de North Carolina, en mayo de 2015.

  17. Control de la calidad del diagnóstico coproparasitológico en la provincia de Ciudad de La Habana, Cuba

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    Angel Núñez Fidel

    1997-01-01

    Full Text Available Se realizó un estudio sobre la calidad del diagnóstico coproparasitológico en 77 laboratorios de la red de salud pública de la provincia Ciudad de La Habana, Cuba. El procedimiento se basó en la entrega a cada jefe de laboratorio de un modelo de encuesta, y una bolsa de nylon conteniendo 10 viales plásticos con distintos especímenes parasitarios, preservados en formaldehído al 7%. Recogidos los resultados en las primeras 72 horas después de su entrega, se realizó la evaluación mediante una escala de puntuación establecida. La mayoría de los laboratorios aprobaron (70%; sin embargo aún existen centros, sobre todo policlínicas, con calificaciones deficientes. Los municipios con resultados más desfavorables fueron, Lisa, Marianao y Habana del Este, alcanzándose mejores resultados en los hospitales que en las policlínicas. En el análisis de Protozooarios, el mejor diagnosticado fué Giardia lamblia, con solo un centro que erró al identificarlo. Las mayores dificultades se presentaron en Blastocystis hominis con 61% de fallas, Endolimax nana, con 24,6%, y Entamoeba histolytica, con 22%. Entre los helmintos, la mayor aprobación fué en Trichuris trichiura y los errores diagnósticos predominaron con Fasciola hepatica y Taenia sp., ambos con 66,2% de fallas. Dados los resultados obtenidos, hemos organizado una intervención educativa en la red de laboratorios de la provincia.

  18. La red sobre trabajo infantil peligroso (Red Tip

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    Walter Varillas

    2003-01-01

    Full Text Available En el mundo, aproximadamente 351.7 millones de niños entre 5 y 17 años realizaban algún tipo de actividad económica, de ellos 170.5 millones (48.5% realizaban algún tipo de trabajo considerado peligroso. Un alto porcentaje se encuentra en la agricultura, otros en minas, manufacturas, ladrilleras, predominantemente en la economía informal. El Convenio 138 (cobre la edad mínima de admisión en el empleo de la OIT y el Convenio 182 (sobre las peores formas de trabajo infantil, definen como trabajo infantil peligroso el que puede afectar la salud, seguridad y moralidad de los menores. Estudios específicos sobre los menores muestran su susceptibilidad particular frente a los riesgos laborales, aumentando la peligrosidad para su normal desarrollo y crecimiento: "los niños no son adultos pequeños". Los profesionales de la seguridad y salud en el trabajo pueden colaborar con los profesionales y las organizaciones especializadas en el trabajo infantil, en la definición y caracterización de lo que significa el trabajo infantil peligroso. Para ello se ha conformado la Red sobre Trabajo Infantil Peligroso (Red TIP, con la finalidad de articular estos dos espacios, orientados a eliminar el trabajo infantil peligroso y rescatar al menor y devolverle la oportunidad de sonreír ahora y en el futuro.

  19. Variações sobre a porta barroca

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    Roger Bastide

    2006-07-01

    Full Text Available Publicado em francês no segundo número da revista Habitat (São Paulo, 1951, com fotografias de Pierre Verger, "Variações sobre a porta barroca" é um ensaio que sintetiza linhas de força da obra de Roger Bastide. Com foco em igrejas da Bahia e de Pernambuco, o autor tece formulações iluminadoras sobre a função sociológica da porta e, por extensão, da ornamentação no barroco brasileiro.First published in French in the second issue of Habitat (São Paulo, 1951, with photographs by Pierre Verger, "Variações sobre a porta barroca" is an essay that is representative of Roger Bastide’s work in Brazil. Taking churches in Bahia and Pernambuco as an example, the author formulates challenging interpretations about the sociologial function of the door and ornaments during Baroque period in Brazil.

  20. AUTOPERCEPCIÓN DE LOS ADULTOS MAYORES SOBRE SU VEJEZ*

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    Sagrario Garay Villegas

    2010-03-01

    Full Text Available El objetivo de este artículo es aproximarse a los significados y percepciones que tienen los adultos mayores sobre esta etapa de su vida. Para ello utilizaremos herramientas de carácter cualitativo y cuantitativo. Nuestra pretensión de usar ambos métodos es contar con la visión de la población con 60 años o más, sobre aspectos relacionados con su salud física y mental, pero también indagar el los factores que podrían estar influyendo en dicha percepción. Los resultados encontrados nos revelan que existe una asociación muy cercana entre la percepción sobre la salud física y el estado mental de las personas adultas mayores. Palabras clave: vejez; significados; salud mental; salud física y felicidad. * Agradecemos los valiosos comentarios a versiones preeliminares de este documento realizados por la Dra. Verónica Montes de Oca.