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Sample records for blastocystis hominis sobre

  1. Observações sobre Blastocystis hominis e Cyclospora cayetanensis em exames parasitológicos efetuados rotineiramente Observations on Blastocystis hominis and Cyclospora cayetanensis in routine parasitological examinations

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    Ruth Semira Rodriguez Alarcón

    2007-04-01

    Full Text Available Relatamos algumas observações, efetuadas com exames parasitológicos de fezes, em atividades rotineiras: os métodos de Faust e cols e de sedimentação espontânea em água não servem para evidenciação de Blastocystis hominis; foram encontradas expressivas porcentagens de presença desse protozoário, sobretudo quando realizada coloração pela hematoxilina férrica; houve 0,7% de registro de positividade para Cyclospora cayetanensis, sugerindo inclusão habitual de pesquisa, por técnicas apropriadas, de tal parasita.We report some observations made from routine parasitological examinations on feces. The methods of Faust et al. and of spontaneous sedimentation in water are not enough to identify Blastocystis hominis. Significant percentage presence of this protozoan was found, especially when staining with iron hematoxylin was performed. Cyclospora cayetanensis was found in 0.7% of the cases, which suggests that this parasite should also routinely be investigated by appropriate techniques.

  2. Characteristics of Factors of Protozoa Blastocystis hominis Persistence.

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    Potaturkina-Nesterova, N I; Il'ina, N A; Bugero, N V; Nesterov, A S

    2016-10-01

    Persistence activity manifested in the expression of anti-lysozyme, anti-lactoferrin, and antihistone factors promoting inactivation of natural anti-infection resistance factors in the body was revealed in Blastocystis hominis protozoa. Activities of these factors were ranged. The frequency of these factors in clinical isolates of blastocyst decreased in the following order: anti-lactoferrin activity (84.5±3.7%)→anti-lysozyme activity (64.8±5.7%)→anti-histone activity (48.1±2.3%). In healthy humans, the corresponding parameters were 7.3±1.3, 5.3±0.9, and 3.3±0.4%, respectively (pprotozoa.

  3. Presencia de Blastocystis Hominis como agente causal de enfermedades gatrointestinales en la comuna 7 (Gaira) del Distrito de Santa Marta

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    Sonja Liliana Lozano Socarras

    2013-01-01

    La Blastocystis hominis es un protozoo que causa cuadros diarreicos. Es altamente prevalente en poblaciones que no cuentan con servicios adecuados de higiene, alcantarillado y salud pública. La infección con Blastocystis hominis frecuentemente concomita con otros enteropatógenos de reconocida patogenicidad, además se ha reportado como parásito oportunista en pacientes con VIII SIDA. El objetivo del presente estudio es determinar la presencia de Blastocystis hominis en pacientes de consulta ex...

  4. Eradication of Blastocystis hominis prevents the development of symptomatic Hashimoto's thyroiditis: a case report.

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    Rajič, Borko; Arapović, Jurica; Raguž, Kazimir; Bošković, Mladen; Babić, Senaida Marina; Maslać, Suzana

    2015-07-30

    In this case report we describe a 49 year-old man who presented with chronic urticaria, angioedema and soft stool consistency. During diagnostic examinations Hashimoto's thyroiditis was found even though the patient never had clear symptoms of this disease. Blastocystis hominis was isolated through a stool microbiologic examination, implicating that this parasite can cause the development of Hashimoto's thyroiditis and chronic urticaria. After two-weeks treatment with metronidazole the Blastocystis hominis was eradicated, then urticaria and angioedema disappeared. During the four years of follow-up, the patient presented without any symptoms, whereas thyroid hormones were normalized and anti-thyroid antibodies declined. For the first time in the literature we show that eradication of Blastocystis hominis can prevent the development of both symptomatic Hashimoto's thyroiditis and chronic urticaria.

  5. Blastocystis hominis and Endolimax nana Co-Infection Resulting in Chronic Diarrhea in an Immunocompetent Male.

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    Shah, Mitanshu; Tan, Christopher Bryan; Rajan, Dhyan; Ahmed, Shadab; Subramani, Krishnaiyer; Rizvon, Kaleem; Mustacchia, Paul

    2012-05-01

    Blastocystis hominis and Endolimax nana exist as two separate parasitic organisms; however co-infection with the two individual parasites has been well documented. Although often symptomatic in immunocompromised individuals, the pathogenicity of the organisms in immunocompetent subjects causing gastrointestinal symptoms has been debated, with studies revealing mixed results. Clinically, both B. hominis and E. nana infection may result in acute or chronic diarrhea, generalized abdominal pain, nausea, vomiting, flatulence and anorexia. We report the case of a 24-year-old immunocompetent male presenting with chronic diarrhea and abdominal pain secondary to B. hominis and E. nana treated with metronidazole, resulting in symptom resolution and eradication of the organisms. Our case illustrates that clinicians should be cognizant of both B. hominis and E. nana infection as a cause of chronic diarrhea in an immunocompetent host. Such awareness will aid in a timely diagnosis and possible parasitic eradication with resolution of gastrointestinal symptoms.

  6. Clinical significance and frequency of Blastocystis hominis in Turkish patients with hematological malignancy.

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    Taşova Y

    2000-06-01

    Full Text Available The effect of Blastocystis hominis (B. hominis in both immunocompetent and immunocompromised subjects has been the subject of debate in recent years, mostly in response to its unknown pathogenicity and frequency of occurrence. We performed a non-randomised, open labelled, single institute study in our hospital in order to investigate the clinical significance and frequency of B. hominis in patients suffering from hematological malignancy (HM who displayed symptoms of gastrointestinal diseases during the period of chemotherapy-induced neutropenia. The presence and potential role of other intestinal inclusive of parasites were also studied. At least 3 stool samples from each of 206 HM patients with gastrointestinal complaints (the HM group were studied. These were compared with stool samples from a control group of 200 patients without HM who were also suffering from gastrointestinal complaints. Samples were studied with saline-lugol, formalin-ether, and trichome staining methods. Groups were comparable in terms of gender, age and type of gastrointestinal complaints. In the HM group, the most common parasite was B. hominis. In this group, 23 patients (13% had B. hominis, while in the control group only 2 patients (1% had B. hominis. This difference was statistically significant (P < 0.05. Symptoms were non-specific for B. hominis or other parasites in the HM group. The predominant symptoms in both groups were abdominal pain (87-89.5%, diarrhea (70-89.5%, and flatulence (74-68.4%. Although all patients with HM were symptom-free at the end of treatment with oral metranidazol (1,500 mg per day for 10 days 2 patients with HM had positive stool samples containing an insignificant number of parasites (< 5 cells per field. In conclusion, it appears that B. hominis is not rare and should be considered in patients with HM who have gastrointestinal complaints while being treated with chemotherapy. Furthermore, metranidazol appears to be effective in

  7. Blastocystis hominis among symptomatic and asymptomatic individuals in Talkha Center, Dakahlia Governorate, Egypt.

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    El-Shazly, Atef M; Abdel-Magied, Aida A; El-Beshbishi, Samar N; El-Nahas, Hala A; Fouad, Mahmoud A H; Monib, Mohamed S M

    2005-08-01

    Blastocystis hominis is now getting acceptance as an agent of human intestinal disease. B. hominis in stool samples of symptomatic and asymptomatic individuals was evaluated as a possible cause of gastro-intestinal troubles. B. hominis was found in 106 (10.1%) out of 1050 individuals examined from six villages and one city in Talkha Center, Dakahlia Governorate. The highest infection rate was in Manshayt El-Badawy village (25.47%), whereas Talkha City showed the lowest rate (4.73%). Age group 10-20 years had higher infection (13.3%). In twenty-three symptomatic patients, B. hominis represented the only causative parasitic agent. The most common symptoms were diarrhoea (30.4%), abdominal pain (26.1%), flatulence (21.7%). vomiting (13.1%) and fatigue (8.7%). High concentrations of B. hominis were found in symptomatic patients than in asymptomatic ones with statistical significant difference (8.2 cells/100 x field versus 3.8 respectively). The mean number of B. hominis was significantly high in patients complaining of diarrhoea and abdominal pain.

  8. Stress exacerbates infectivity and pathogenicity of Blastocystis hominis: in vitro and in vivo evidences.

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    Samudi Chandramathi

    Full Text Available BACKGROUND: Stress alters the oxidant-antioxidant state and immune cell responses which disrupts its function to combat infection. Blastocystis hominis, a common intestinal protozoan has been reported to be opportunistic in immunocompromised patients namely cancer. B. hominis infectivity in other altered immune system conditions especially stress is unknown. We aimed to demonstrate the stress effects towards the susceptibility and pathogenicity of B. hominis infection. METHODS/FINDINGS: Three-week-old Wistar rats were divided into four groups: (acontrol; (bstress-induced; (cB. hominis infected; (dstress-induced with B. hominis infection; (n = 20 respectively. Stress was induced for an hour daily (30 days using a Belly Dancer Shaker. Weight gain was monitored, stool samples were collected for B. hominis screening and blood for the determination of differential count, levels of immunoglobulin, oxidative damage, and peripheral blood mononuclear cell (PBMC proliferation upon induction with solubilized antigen of B. hominis (Blasto-Ag. Group (b exhibited the highest level of weight gain. Group (d had higher levels of parasite cyst count in stools, serum IgE, oxidized protein and lipid compared to the group (c. Levels of monocyte and antioxidant in group (d were decreased and their PBMCs showed highest inhibition of proliferation level when exposed to Blasto-Ag. Monocyte level in Group (b showed insignificant difference compared to group (a but was significantly lower compared to group (c. Antioxidant levels in group (c were generally lower compared to group (a and (b. Inhibition level exhibited by Blasto-Ag treated PBMCs of group (c was higher compared to group (a and (b. CONCLUSION: The pathogenicity and augmentation of B. hominis infection is enhanced when stress is present. Lifestyles today are becoming increasingly stressed and the present findings suggest that the parasite which has been reported to be one of the most common organisms seen in

  9. [Prevalence of Blastocystis hominis among food handlers from Caroni municipality, Bolivar State, Venezuela].

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    Requena, Ixora; Hernández, Yessica; Ramsay, Mario; Salazar, Carmen; Devera, Rodolfo

    2003-01-01

    A cross-sectional survey was conducted to determine the prevalence of Blastocystis hominis infection in a random sample of apparently healthy food handlers. A total of 415 individuals attending the Manoa Urban Outpatient Clinic (Caroní Municipality, Bolívar State, Venezuela) in the Adult Hygiene Program and who requested health certification to work as food handlers were studied. Stool samples obtained by spontaneous evacuation were examined by direct microscopy and the Willis concentration method. A total of 150 individuals were infected (36.14%), 107 (25.78%) of whom with B. hominis. There was no difference between males and females (p > 0.05), but there was a significant difference between ages (chi(2) = 12.17; g.l. = 4), with infection more frequent between 18 and 27 years. In 71.02% of the cases, B. hominis was the only parasite. Giardia lamblia was the parasite most frequently associated with B. hominis (2.41%). In the majority (85%) of infected individuals, less than five microorganisms per microscopic field were observed. We conclude that B. hominis is a frequent intestinal parasite among food handlers in Caroní Municipality, Bolivar State, Venezuela.

  10. The prevalence of Blastocystis hominis and other protozoan parasites in soldiers returning from peacekeeping missions.

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    Duda, Aleksandra; Kosik-Bogacka, Danuta; Lanocha-Arendarczyk, Natalia; Kołodziejczyk, Lidia; Lanocha, Aleksandra

    2015-04-01

    Blastocystis hominis is a common intestinal parasite found in humans living in poor sanitary conditions, living in tropical and subtropical climates, exposed to infected animals, or consuming contaminated food or water. The aim of this study was to determine the prevalence of B. hominis in Polish military personnel returning from peacekeeping missions in Iraq and Afghanistan. In total, 1,826 stool samples were examined. Gastrointestinal parasites were detected in 17% of the soldiers. The examined stool samples most frequently contained vacuolar forms of B. hominis (15.3%) and cysts of Entamoeba coli (1.0%) or Giardia lamblia (0.7%). In 97.1% of stool samples from infected soldiers, we observed less than five developmental forms of B. hominis in the field of view (40×). The parasite infections in soldiers were diagnosed in the autumn and the spring. There was no statistical correlation between age and B. hominis infection. Our results show that peacekeeping missions in countries with tropical or subtropical climates could be associated with risk for parasitic diseases, including blastocystosis.

  11. Investigation of the blastocystis hominis frequency in patients with irritable bowel syndrome

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    Bayram Pektaş

    2014-06-01

    Full Text Available AimIn this study, it was aimed to investigate the relationship between Blastocystis hominis infection and inflammatory bowel syndrome (IBS. Methods: In this study, the frequency of B. hominis in the stool samples of 52 patients applied to Microbiology laboratory and pre-diagnosed with irritable bowel syndrome in January 2013-June 2013 was investigated, retrospectively. Microscopic investigations were evaluated after macroscopic examination. For this purpose, the stool samples of the diarrheal cases were investigated by trichrome staining after they were prepared by native-lugol and formol ethyl acetate concentration method. The results were compared with the examination of 2160 stool samples sent to our laboratory during the same period. Results: Stool samples of 52 patients pre-diagnosed with IBS were accepted to our laboratory in January 2013-June 2013. 13 of the patients were found as B. hominis positive. Weight loss and anorexia was identified only in one patient while abdominal pain, diarrhea and gas complaints were identified in all of the IBH and B. hominis positive patients. During the same period, parasites were detected in 96 (4.4% of 2160 stool samples sent to our laboratory and the most common was B. hominis 48 (2.2%. 452 of these patients applied with diarrhea symptoms and B. hominis was detected in 36 samples (7.96%. Conclusion: The limited studies investigating the presence of B. hominis in patients with irritable bowel syndrome are far from illuminating the role of this agent in disease pathogenesis. We believe that further investigations should be performed. In this study, 25% of the patients were found as positive. J Clin Exp Invest 2014; 5 (2: 242-245

  12. Presencia de Blastocystis Hominis como agente causal de enfermedades gatrointestinales en la comuna 7 (Gaira del Distrito de Santa Marta

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    Sonja Liliana Lozano Socarras

    2013-10-01

    Full Text Available La Blastocystis hominis es un protozoo que causa cuadros diarreicos. Es altamente prevalente en poblaciones que no cuentan con servicios adecuados de higiene, alcantarillado y salud pública. La infección con Blastocystis hominis frecuentemente concomita con otros enteropatógenos de reconocida patogenicidad, además se ha reportado como parásito oportunista en pacientes con VIII SIDA. El objetivo del presente estudio es determinar la presencia de Blastocystis hominis en pacientes de consulta externa con síntomas asociados a enfermedades gastrointestinales, en la comuna 7 del distrito de Santa Marta, Colombia, durante el mes de Enero a Diciembre de 2004. El método de diagnóstico utilizado fue examen coproparasitológico seriado y el número de pacientes analiza-dos fue de 291. Los resultados muestran una alta presencia de Blastocystis hominken pacientes con enfermedad diarreica residentes en Gaira. Un alto porcentaje de la población parasitada (62,6% presento como único agente causal de la enfermedad diarreica al Blastocystis hominis lo que sugiere la presencia de otras enfermedades que pueden involucrar un compromiso inmunológico, el efecto será una respuesta inmune débil contra el parásito.

  13. Epidemiological survey of Blastocystis hominis in Huainan City, Anhui Province, China

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    Ke-Xia Wang; Chao-Pin Li; Jian Wang; Yu-Bao Cui

    2002-01-01

    AIM: To provide scientific evidence for prevention andcontrolling of blastocystosis, the infection of Blastocystishomonis and to study its clinical significance in Huainan City,Anhui Province, China.METHODS: Blastocystis homonis in fresh stools taken from100 infants, 100 pupils, 100 middle school students and403 patients with diarrhea was smeared and detected withmethod of iodine staining and hematoxylin staining. Afterpreliminary direct microscopy, the shape and size ofBlastocystis homonis were observed with high power lens.The cellular immune function of the patients withblastocystosis was detected with biotin-streptavidin (BSA).RESULTS: The positive rates of Blastocystis homonis inflesh stools taken from the infants, pupils, middle schoolstudents and the patients with diarrhea, were 1.0 % (1/100),1.0 % (1/100), 0 % (0/100) and 5.96 % (24/403)respectively. Furthermore, the positive rates of Blastocystishomonis in the stool samples taken from the patients withmild diarrhea, intermediate diarrhea, severe diarrhea andobstinate diarrhea were 6.03 % (14/232), 2.25 % (2/89),0 % (0/17) and 12.31% (8/65) respectively. The positiverates of Blastocystis homonis in fresh stools of male andfemale patients with diarrhea were 7.52 % (17/226) and3.95 % (7/177) respectively, and those of patients in urbanand rural areas were 4.56 % (11/241) and 8.02 % (13/162)respectively. There was no significant difference betweenthem (P>0.05). The positive rates of CD3+, CD4+, CD8+ inserum of Blastocystis homonis-positive and-negativeindividuals were 0.64±0.06, 0.44±0.06, 0.28±0.04 and0.60±0.05, 0.40±0.05 and 0.30±0.05 respectively, andthe ratio of CD4+/CD8+ of the two groups were 1.53±0.34and 1.27±0.22. There was significant difference betweenthe two groups (P<0.05, P<0.01).CONCLUSION: The prevalence of Blastocystis hominis asan enteric pathogen in human seems not to be associatedwith gender and living environment, and that Blastocystishominis is more common in stool samples of

  14. Frequency of Blastocystis hominis and other intestinal parasites in stool samples examined at the Parasitology Laboratory of the School of Pharmaceutical Sciences at the São Paulo State University, Araraquara Freqüência de Blastocystis hominis e outros enteroparasitas em amostras de fezes examinadas no Laboratório de Parasitologia da Faculdade de Ciências Farmacêuticas da Universidade Estadual Paulista, Araraquara

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    Júlio César Miné

    2008-12-01

    como comparar diferentes métodos para a pesquisa desse protozoário em amostras de fezes. Foram estudadas 503 amostras de fezes submetidas ao exame direto a fresco, às técnicas de Faust e cols, Lutz e de Rugai e cols, além das colorações pela hematoxilina férrica, tricrômio e de Kinyoun modificada. Entre as 503 amostras examinadas, 174 (34,6% apresentaram-se positivas para a presença de parasitas intestinais. O protozoário e o helminto mais freqüentes foram Entamoeba coli (14,6% e Strongyloides stercoralis (6,7%, respectivamente. Blastocystis hominis foi observado em 23 (4,6% amostras fecais com consistência predominantemente pastosa, não caracterizando quadro diarréico. Apesar da baixa freqüência de Blastocystis hominis encontrada na região de Araraquara, comparativamente a outras regiões brasileiras, é importante a realização do diagnóstico laboratorial desse protozoário. O encontro de Blastocystis hominis em material fecal é indicativo de contaminação de alimentos e água de consumo, desde que se admita a rota de transmissão oral-fecal desse parasita, o que implica na orientação da população sobre as medidas de saneamento básico e higiene como meio para se controlar problemas de saúde ocasionados pelos enteroparasitas.

  15. Prevalence and Risk Factors of Giardia lamblia and Blastocystis hominis Infections in Children Under Ten Years Old, Hamadan, Iran

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    Sedighi

    2015-05-01

    Full Text Available Background Nowadays, parasitic infections are a major health problem throughout the world, particularly in the developing countries. Objectives Considering the high susceptibility of children against parasitic infections, the current study aimed to determine the prevalence and associated risk factors of intestinal parasitic infections among children less than 10 years old in urban and rural areas of Hamadan district. Patients and Methods The current study was conducted on 395 children (214 males and 181 females, referred to urban and rural health centers in Hamadan district in 2013. Stool samples were examined by formalin-ether concentration technique, and trichrome and modified Ziehl-Neelsen staining methods. The results were analyzed by chi-square test. Results Of the 395 studied children, 112 (28.4% were infected with intestinal parasites. Blastocystis hominis was the most frequently detected parasite with the prevalence of 18.5%, followed by Giardia lamblia (10.9%, Entamoeba coli (2.8%, Dientamoeba fragilis (0.8%, Iodamoeba buetschlii (0.8%, Chilomastix mesnili (0.5%, Cryptosporidium spp. (0.5%, Endolimax nana (0.3% and Entamoeba hartmanni (0.3%. No cases of infection with helminth parasites were found. Conclusions The results of the study showed a high prevalence of Giardia lamblia and Blastocystis hominis in rural areas compared to urban regions. Therefore it is necessary to promote the public health awareness in the rural population, in order to reduce the frequency of parasitic infections.

  16. Prevalencia de Blastocystis hominis en vendedores ambulantes de comida del municipio Caroní, Estado Bolívar, Venezuela Prevalence of Blastocystis hominis among food handlers from Caroni municipality, Bolivar State, Venezuela

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    Ixora Requena

    2003-12-01

    Full Text Available Para determinar la prevalencia de Blastocystis hominis en una muestra de vendedores ambulantes de comida, aparentemente sanos, se realizó un estudio seccional con 415 personas que acudieron al Ambulatorio Urbano tipo III "Manoa" (Municipio Caroní, Estado Bolívar, Venezuela, Programa de Higiene del Adulto, a solicitar el certificado de salud para trabajar como vendedores de comida. Una muestra de heces obtenida por evacuación espontánea fue analizada mediante la técnica de examen directo y método de concentración de Willis. Se encontraron 150 personas parasitadas (36,14%, de ellas 107 (25,78% con B. hominis. No se observó predilección por el sexo (p > 0,05, pero sí con relación a la edad, siendo las personas de 18 a 27 años las más afectados (ji² = 12,17; g.l. = 4. En el 71,02% de los casos se encontró como parásito único y en 28,98% de los casos asociados a otros parásitos, siendo el más frecuentemente asociado Giardia lamblia (2,41%. En la mayoría de las personas parasitadas (85% el protozoario se observó en un número menor de cinco células por campo. Se concluye que B. hominis es un parásito frecuente en manipuladores de alimentos del Municipio Caroní, Estado Bolívar, Venezuela.A cross-sectional survey was conducted to determine the prevalence of Blastocystis hominis infection in a random sample of apparently healthy food handlers. A total of 415 individuals attending the Manoa Urban Outpatient Clinic (Caroní Municipality, Bolívar State, Venezuela in the Adult Hygiene Program and who requested health certification to work as food handlers were studied. Stool samples obtained by spontaneous evacuation were examined by direct microscopy and the Willis concentration method. A total of 150 individuals were infected (36.14%, 107 (25.78% of whom with B. hominis. There was no difference between males and females (p > 0.05, but there was a significant difference between ages (chi² = 12.17; g.l. = 4, with infection more

  17. Prevalencia de Blastocystis hominis en vendedores ambulantes de comida del municipio Caroní, Estado Bolívar, Venezuela

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    Requena Ixora

    2003-01-01

    Full Text Available Para determinar la prevalencia de Blastocystis hominis en una muestra de vendedores ambulantes de comida, aparentemente sanos, se realizó un estudio seccional con 415 personas que acudieron al Ambulatorio Urbano tipo III "Manoa" (Municipio Caroní, Estado Bolívar, Venezuela, Programa de Higiene del Adulto, a solicitar el certificado de salud para trabajar como vendedores de comida. Una muestra de heces obtenida por evacuación espontánea fue analizada mediante la técnica de examen directo y método de concentración de Willis. Se encontraron 150 personas parasitadas (36,14%, de ellas 107 (25,78% con B. hominis. No se observó predilección por el sexo (p > 0,05, pero sí con relación a la edad, siendo las personas de 18 a 27 años las más afectados (ji² = 12,17; g.l. = 4. En el 71,02% de los casos se encontró como parásito único y en 28,98% de los casos asociados a otros parásitos, siendo el más frecuentemente asociado Giardia lamblia (2,41%. En la mayoría de las personas parasitadas (85% el protozoario se observó en un número menor de cinco células por campo. Se concluye que B. hominis es un parásito frecuente en manipuladores de alimentos del Municipio Caroní, Estado Bolívar, Venezuela.

  18. Clinical analysis of blastocystis hominis infection in children%小儿人芽囊原虫感染53例临床分析

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    胡缨; 陈国力; 李艳文

    2012-01-01

    目的:探讨小儿人芽囊原虫感染的临床特点及诊治方法.方法:对我院儿科收治的53例确诊为人芽囊原虫感染的临床资料进行分析.结果:47例患儿有消化道症状:腹泻31例,腹痛27例.患儿粪便中均查见人芽囊原虫,以空泡型最多见.实验室辅助检查中,66.04%患儿血红蛋白降低,3.77%患儿嗜酸性粒细胞升高.81.13%患儿经甲硝唑1个疗程治愈,其余2个疗程治愈,且3个月后复查均无复发.结论:对有消化道症状而病因不明的患儿须高度警惕人芽囊原虫感染,酌情配合使用直接涂片法和碘液染色法是确诊简单有效的途径.甲硝唑治疗效果良好.%Objective To Investigate the clinical characteristics and diagnostic methods of Blastocystis Hominis infection in children. Methods 53 children with Blastocystis Hominis infection admitted in the Pediatric Department were retrospectively analyzed. Results Alimentary disorders were found in 47 children, including diarrhea in 31 cases and abdominal pain in 27 cases. Blastocystis Hominis was found in all stool specimens with vacuolus type which is the most common. hemoglobin was decreased in 66.04% of patients, eosinophilic granulocytes was increased in 3.77%. So did the left 18.87% of patients after two courses. No recurrence was found 3 months later. Conclusion Blastocystis Hominis infection should be highly suspected in children with alimentary disorders but without obvious causes. Direct smear and Iodine staining are the simple and effective methods. Metronidazole can achieve good effect.

  19. The use of the antifungal agent miconazole as an inhibitor of Blastocystis hominis growth in Entamoeba histolytica/E. dispar cultures Uso do antifúngico miconazol como inibidor do crescimento de Blastocystis hominis em culturas de Entamoeba histolytica/E. dispar

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    Alessandra Queiroga Gonçalves

    2007-06-01

    Full Text Available In regions with high prevalence, Blastocystis hominis is frequently found in association with Entamoeba histolytica/E. dispar in xenic cultures. Its exacerbated growth is often superimposed on the growth of amebas, thus impeding the continuation of the amebas in the culture, within a few generations. The present study reports on the excellent efficacy (100% of the antifungal agent miconazole in eliminating B. hominis from cultures of E. histolytica/E. dispar, thereby maintaining the integrity of the trophozoites of the amebas. Nystatin presented low efficacy (33.3%.Em regiões de alta prevalência, Blastocystis hominis é freqüentemente encontrado em associação com Entamoeba histolytica/E. dispar em cultivos xênicos. Seu crescimento exacerbado se sobrepõe muitas vezes ao das amebas, impedindo a manutenção destas em cultura, dentro de poucas gerações. O presente estudo relata a excelente eficácia (100% do antifúngico miconazol na eliminação de B. hominis dos cultivos de E. histolytica/E. dispar, mantendo-se a integridade dos trofozoítos das amebas. A nistatina apresentou eficácia baixa (33,3%.

  20. 人芽囊原虫多样化繁殖方式短暂共存现象%Temporary coexistence phenomenon of diversified reproductive mode of Blastocystis hominis

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    张红卫; 贺丽君; 李文; 颜秋叶; 苏云普; 薛长贵; 王东

    2005-01-01

    目的为了解人芽囊原虫对外界条件的适应性,观察培养条件改变对其形态和繁殖方式的影响. 方法从腹泻患者粪便中检查并分离培养获得人芽囊原虫,分别用locke氏-鸡蛋双相培养基(LES)和改良1640培养基(RPMI-1640)培养,改变培养条件,观察人芽囊原虫形态和繁殖方式. 结果在两种培养基中均可见到人芽囊原虫的四种主要形态:空泡型、颗粒型、阿米巴型和包囊型,其繁殖方式以二分裂为主,可见少量出芽生殖.当从LES培养基突然变为RPMI-1640培养基时,人芽囊原虫出现短时间(48~72h)内生长停滞,随后出现多种形式的繁殖方式,可见较多的二分裂、出芽生殖、三分裂、多分裂、裂体生殖,持续24h后恢复为二分裂为主. 结论培养条件改变能够引起人芽囊原虫繁殖方式的改变,出现短暂性多样化繁殖方式,人芽囊原虫对环境的适应能力很强.%Objective To understand the adaptability of Blastocystis hominis (B. hominis ) to environment, observe effect of culture condition change on reproductive mode of B. hominis. Methods B. hominis was isolated from patient with diarrhea and cultured in different culture medium including Locke-egg-serum medium culture (LES) and modified RPMI-1640 culture medium (RPMI-1640). When culture condition was changed between LES and RPMI-1640, morphous and reproductive modes were observed. Results All of 4 form including vacuolar form, granular form, ameba form and cyst form were found in each culture medium, separately, and main reproductive mode is binary fission, a few of gemmation was exist. When culture condition was changed abruptly from LES medium to RPMI-1640 medium, different reproductive mode including binary fission, ternary fission,multiple fission and gemmation were found after growth retardation of 24 ~ 72 hours. This phenomenon continued less than 24 hours and binary fission remained reproductive mode then. Conclusion Reproductive

  1. Comparative study of frequency of Blastocystis hominis pre-schoolers from an urban area and rural area of Cartagena de Indias and its relation with signs and symptoms and risk factors

    Directory of Open Access Journals (Sweden)

    Mavianis Pinilla Pérez

    2015-08-01

    Full Text Available Background: the intestinal parasitism is common infections in the world and of higher prevalence in the poor community of developing countries. Until ending of decade of eighties, Blastocystis hominis is not reported in a routine way, being only a differential diagnostic problem; but in the nineties by means of an appropriate diagnosis increase its prevalence of 1.5 to 10 % in developed countries and with 30 to 50 % in developing countries. Objective: to compare frequency of Blastocystis hominis in preschoolers from an urban area and rural area of Cartagena de Indias and its relation with signs and symptoms and risk factors during the year 2013. Materials and methods: 286 samples of feces of children between 1 and 5 years old from an urban area (Crespo and rural area (La Boquilla small town were analyzed by serial coprological technique. Results: were found parasites in 38.5% (55/143 of children from urban area and 79% (113/143 of children from rural area. It was found association between presence of parasites with origin of children (X2= 48.53; p<0.0001. We reported a frequency of 51.1% de B. hominis in an urban area and 44.8 % in a rural area. Conclusions: the absence of monitoring and control of cases suggests the need to formulate and implement measurements guided to improve the quality of life in studied areas.

  2. Parasitoses intestinais de indígenas da comunidade Mapuera (Oriximiná, Estado do Pará, Brasil: elevada prevalência de Blastocystis hominis e encontro de Cryptosporidium sp e Cyclospora cayetanensis Intestinal parasitosis in Indians of the Mapuera community (Oriximiná, State of Pará, Brazil: high prevalence of Blastocystis hominis and finding of Cryptosporidium sp and Cyclospora cayetanensis

    Directory of Open Access Journals (Sweden)

    Jaila Dias Borges

    2009-06-01

    Full Text Available Foi avaliada a ocorrência de parasitoses intestinais em indígenas da aldeia Mapuera (Oriximiná, Estado do Pará, Brasil. No contexto de apreciações congêneres, expressa contribuição para adequado conhecimento do assunto, significativo sob o ponto de vista médico-sanitário. O exame parasitológico das fezes, de 83 pessoas, realizado por meio de quatro métodos, pode ser considerado como dotado de razoável amplitude para estabelecer diagnósticos. Ocorreu encontro de cistos de protozoários e de ovos de helmintos de múltiplos tipos, até mesmo em expressivas porcentagens, merecendo destaque a muito freqüente presença de Blastocystis hominis (57,8%, como também o encontro de Cryptosporidium sp (3,6% e de Cyclospora cayetanensis (10,8%, comentado especificamente. O verificado demonstra que tais índios vivem em ambiente onde prevalecem más condições higiênicas, em especial, facilitador da disseminação de protozoários e helmintos pelo contato com o solo ou ingestão de água e alimentos contaminados.Occurrences of intestinal parasitosis in Indians of the Mapuera community (Oriximiná, State of Pará, Brazil were evaluated. Within the context of group assessment, this study makes a contribution towards adequate knowledge of this subject, which is significant from a medical-sanitary point of view. Parasitological examination of feces from 83 individuals, performed using four different methods, could be considered to have reasonable amplitude for establishing diagnoses. Protozoan cysts and helminth eggs of many types were found, even with significant percentages. The frequent presence of Blastocystis hominis (57.8%, along with findings of Cryptosporidium sp (3.6% and Cyclospora cayetanensis (10.8%, deserved highlighting with specific comments. The findings show that these Indians live in an environment in which poor hygiene conditions prevail. In particular, these facilitate the dissemination of protozoa and helminths through

  3. Survey on Blastocystis hominis infection in HIV positive individuals in Fuyang City, Anhui Province%安徽阜阳HIV阳性者合并人芽囊原虫感染及其影响因素分析

    Institute of Scientific and Technical Information of China (English)

    田利光; 王珍丽; 汪天平; 周晓农; 陈家旭; 程国金; 汪峰峰; 郭俭; 尹晓梅; 蔡玉春; 周莉; 童小妹

    2012-01-01

    Objective To understand the epidemiological characteristics of co-infection of HIV and Blastocystis hominis and its risk factors. Methods A total of 309 people with HIV positive in the development zone of Fuyang City were recruited, and the face to face questionnaires were conducted to collect the information of behavioral characteristics and sociodemographic data of the participants. Meanwhile, the samples of stool and blood were collected to test B. hominis, cytokines and CD4+/CD8+ T-lymphocyte. The influencing factors of co-infection of HIV and Blastocystis hominis were analyzed by the single factor analysis and Logistic regression analysis. Results Among the 309 people involved, 302 accepted feces examinations, 286 accepted the questionnaire investigation, and 263 accepted both of them. The infection rate of B. hominis was 17.11%, that of the female was 21.90%, which was significantly higher than that of the male (11.90%) (P < 0.05). The results from the multivariale Logistic regression model showed that good nutrition was significantly associated with the co-infection of HIV and B. hominis (OR = 0.263, 95% CI: 0.073, 0.945). Conclusions The infection rate of B. hominis is high in people with HIV positive, and the nutrition situation of individuals may be one of the important risk factors associated with co-infection.%目的 了解安徽省阜阳市HIV阳性者合并人芽囊原虫感染情况及其影响因素.方法 采用横断面调查的方法对安徽省阜阳市开发区309名HIV阳性者进行问卷调查,并采集其粪便和血液样本用于人芽囊原虫、CD4+T淋巴细胞和细胞因子检测.采用单因素分析和Logistic回归分析对影响HIV和人芽囊原虫合并感染的因素进行分析.结果 在309名参与调查的HIV阳性者中,302人参与粪样检测,286人参与问卷调查,同时参与2项检测者263人.调查发现,HIV阳性者合并人芽囊原虫感染率为17.11%,其中女性感染率为21.90%,明显高于男性(11

  4. Morphology of Blastocystis sp. isolated from circus animals.

    Science.gov (United States)

    Stenzel, D J; Cassidy, M F; Boreham, P F

    1993-08-01

    Blastocystis sp. is reported for the first time from faecal samples collected from a camel, a llama, a highland bull and a lion in a travelling circus. Fresh faecal specimens were examined by light and electron microscopy, and vacuolar and cyst forms of similar morphology were present in all three ungulates. These cells were smaller than cultured vacuolar cells of Blastocystis hominis isolated from humans and contained only a single vacuole in comparison to the multivacuolar cell found in fresh human faeces. The taxonomic relationship of Blastocystis isolated from humans and ungulates remains to be determined. The number of parasites present in the lion sample was too small to make valid comparisons.

  5. 人芽囊原虫病患者肠黏膜表皮生长因子水平及其临床意义%Intestinal mucosa of epidermal growth factor in patients with Blastocystis hominis infection and its clinical significance

    Institute of Scientific and Technical Information of China (English)

    俞开敏

    2011-01-01

    目的 检测人芽囊原虫感染者肠黏膜表皮生长因子(EGF)的水平,探讨EGF与肠黏膜损伤程度的关系.方法 感染组为确诊人芽囊原虫感染者44例,对照组为健康体检者40例,均经电子肠镜检查肠黏膜病变情况,并用ELISA法检测肠黏膜组织匀浆EGF水平.结果 电子肠镜检查可见人芽囊原虫感染者的肠黏膜有不同程度病变,表现为充血、水肿、糜烂、溃疡等.感染组EGF为(559.60±178.5)pg/ml,对照组为(977.58±215.5)pg/ml,差异有统计学意义(t=9.7136,P<0.01).且EGF水平随着肠黏膜病变严重程度的增加而逐渐降低.结论 人芽囊原虫感染能引起患者肠黏膜的病理改变及肠黏膜EGF水平降低.人芽囊原虫感染所致肠黏膜损伤可能与原虫的侵入及黏膜中EGF的减少有关.EGF在人芽囊原虫病肠道损伤的修复中起重要作用.%Objective To detect epidermal growth factor (EGF) in intestinal mucosa in patients with Blastocystis hominis infection and explore the relationship between EGF and intestinal mucosa lesions. Methods Forty-four patients who were diagnosed with B. Hominis infection served as the infected group and 40 healthy persons served as the control group. Endoscopy and intestinal biopsy were performed to observe pathological changes in the intestine. The patients'and the controlsintestinal tissue homogenate were tested for EGF with ELISA assays. Results Intestinal mucosa lesions were observed in all patients infected with B. Hominis. Pathological manifestations included mucosal congestion, edema, erosion, and ulceration. The level of EGF in the infected group was (559. 60±178. 5) pg/ml, and that in the control was (977. 58±215. 5) pg/ml. The difference between the two was significant (t=9. 7136,P<0. 01). Furthermore, the level of EGF gradually decreased with the severity of intestinal mucosa lesions. Conclusion B. Hominis infection can cause intestinal mucosa lesions and reduce levels of EGF in the intestinal

  6. Dermatobia hominis infestation.

    OpenAIRE

    Nunzi, E.; Rongioletti, F; Rebora, A

    1984-01-01

    A patient is reported who, after leaving Venezuela, developed some boils on the left upper limb inhabited by Dermatobia hominis larvae. The curious life-cycle of this tropical fly is described with some considerations about the diagnostic problem. A simple unreported way of larvae extraction is suggested.

  7. Dermatobia hominis infestation.

    Science.gov (United States)

    Nunzi, E; Rongioletti, F; Rebora, A

    1984-02-01

    A patient is reported who, after leaving Venezuela, developed some boils on the left upper limb inhabited by Dermatobia hominis larvae. The curious life-cycle of this tropical fly is described with some considerations about the diagnostic problem. A simple unreported way of larvae extraction is suggested.

  8. A retrospective metagenomics approach to studying Blastocystis.

    Science.gov (United States)

    Andersen, Lee O'Brien; Bonde, Ida; Nielsen, Henrik Bjørn; Stensvold, Christen Rune

    2015-07-01

    Blastocystis is a common single-celled intestinal parasitic genus, comprising several subtypes. Here, we screened data obtained by metagenomic analysis of faecal DNA for Blastocystis by searching for subtype-specific genes in coabundance gene groups, which are groups of genes that covary across a selection of 316 human faecal samples, hence representing genes originating from a single subtype. The 316 faecal samples were from 236 healthy individuals, 13 patients with Crohn's disease (CD) and 67 patients with ulcerative colitis (UC). The prevalence of Blastocystis was 20.3% in the healthy individuals and 14.9% in patients with UC. Meanwhile, Blastocystis was absent in patients with CD. Individuals with intestinal microbiota dominated by Bacteroides were much less prone to having Blastocystis-positive stool (Matthew's correlation coefficient = -0.25, P metagenomics approach. The study serves as an example of how it is possible to retrospectively investigate microbial eukaryotic communities in the gut using metagenomic datasets targeting the bacterial component of the intestinal microbiome and the interplay between these microbial communities.

  9. Molecular Epidemiology of Giardia, Blastocystis and Cryptosporidium among Indigenous Children from the Colombian Amazon Basin

    Science.gov (United States)

    Sánchez, Angie; Munoz, Marina; Gómez, Natalia; Tabares, Juan; Segura, Laura; Salazar, Ángela; Restrepo, Cristian; Ruíz, Miguel; Reyes, Patricia; Qian, Yuchen; Xiao, Lihua; López, Myriam C.; Ramírez, Juan D.

    2017-01-01

    The incidence and prevalence of intestinal parasites in children is most likely due to lack of natural or acquired resistance and differences in behavior and habits closely related to environmental and socioeconomic determinants. The most important protozoa that parasitize humans are Giardia, Entamoeba, Blastocystis, and Cryptosporidium. These parasites present wide intraspecific genetic diversity and subsequently classified into assemblages and subtypes. The Amazon basin is the largest in the world and is the fifth freshwater reserve on the planet. Contradictorily, people living in these areas (Indigenous populations) have poor quality of life, which favors the infection of diseases of fecal-oral transmission. The aim of this work was to unravel the molecular epidemiology of Giardia, Blastocystis and Cryptosporidium across four communities (Puerto Nariño, San Juan del Soco, Villa Andrea and Nuevo Paraíso). We obtained 284 fecal samples from children under 15 years old that were analyzed by direct microscopy (261 samples) and Real Time PCR (qPCR) (284 samples). The positive samples for these protozoa were further characterized by several molecular markers to depict assemblages and subtypes. We observed a frequency of Giardia infection by microscopy of 23.7% (62 samples) and by qPCR of 64.8% (184 samples); for Blastocystis by microscopy of 35.2% (92 samples) and by qPCR of 88.7% (252 samples) and for Cryptosporidium only 1.9% (5 samples) were positive by microscopy and qPCR 1.8% (5 samples). Regarding the Giardia assemblages, using the glutamate dehydrogenase (gdh) marker we observed AI, BIII and BIV assemblages and when using triose phosphate isomerase (tpi) we observed assemblages AI, AII, BIII and BIV. In contrast, Blastocystis STs detected were 1, 2, 3, 4, and 6. Lastly, the species C. viatorum, C. hominis (with the subtypes IdA19 and IaA12R8) and C. parvum (with the subtype IIcA5G3c) were identified. We observed a high profile of zoonotic transmission

  10. Diversity of Blastocystis subtypes in dogs in different geographical settings

    DEFF Research Database (Denmark)

    Wang, Wenqi; Cuttell, Leigh; Bielefeldt-Ohmann, Helle

    2013-01-01

    Background: Blastocystis is a ubiquitous, globally distributed intestinal protist infecting humans and a wide range of animals. Several studies have shown that Blastocystis is a potentially zoonotic parasite. A 1996 study reported a 70% Blastocystis prevalence in Brisbane pound dogs while another...... study found that pet dogs/cats of 11 symptomatic Blastocystis infected patients harboured at least one Blastocystis subtype (ST) in common with the patient. These results raised the possibility that dogs might be natural hosts of Blastocystis. In this study, we aimed to investigate this hypothesis...... by estimating the prevalence of Blastocystis carriage and characterising the diversity of STs in dogs from three different environmental settings and comparing these STs with the range that humans harbour. Methods: Two hundred and forty faecal samples from dogs from three different geographical regions...

  11. Blastocystis in a Gut Microbia Context and Aspects of Blastocystis Genomics

    DEFF Research Database (Denmark)

    Andersen, Lee Poul O'Brien

    in order to infer conclusions on pathogenicity. The second study of this thesis attempts to determine if the vast amount of existing metagenomics data could be used as a resource for Blastocystis prevalence and molecular epidemiology studies. A co-abundance binning method was applied, and Blastocystis...... information extracted. The results illustrate that the prevalence of Blastocystis in healthy individuals is similar to that observed in patients with gastrointestinal symptoms, including inflammatory bowel disease, or even higher, and that the parasite is negatively associated to gut microbiota predominated...... by Bacteroidetes. The latter result was followed up and confirmed by the third study, which utilized a quantitative detection technique. Lastly, several different approaches exist with regard to determining the potential pathogenicity of Blastocystis, one of which includes identification and characterization...

  12. In Nomine Hominis

    Directory of Open Access Journals (Sweden)

    Robson Lacerda Dutra

    2010-01-01

    Full Text Available A partir da posição do intelectual e de relações de poder, este texto reflete sobre dois romances de José Saramago centrados em textos bíblicos: Evangelho segundo Jeseus Cristo, publicado em 2001 e Caim, em 2009 e das estratégias ficcionais deste autor para novas formas de leitura e de interpretação do mundo contemporâneo.

  13. Cutaneous myiasis from Dermatobia hominis.

    Science.gov (United States)

    Guse, S T; Tieszen, M E

    1997-08-01

    We present a case report of cutaneous myiasis in a foreign traveler who was infected by Dermatobia hominis while visiting South America. This patient developed a painful furuncular lesion on the anterior scalp and noted that the lesion drained a serosanguinous fluid for more than a month before definitive treatment. Invasion of mammalian tissue by the larval forms of D. hominis typically results in the formation of a classic furuncular lesion. For persons who present with a lesion that contains a central draining stoma located on an exposed body surface, the diagnosis of myiasis should always be considered. In addition to the case report, we present a discussion of furuncular myiasis and describe the peculiar life cycle of the human botfly. We also describe the various therapies that may be employed for treating cutaneous myiasis, including surgical extraction of the larva and asphyxiation of the larva by application of petroleum jelly or other fat derivatives to the central stoma or breathing aperture.

  14. Blastocystis phylogeny among various isolates from humans to insects.

    Science.gov (United States)

    Yoshikawa, Hisao; Koyama, Yukiko; Tsuchiya, Erika; Takami, Kazutoshi

    2016-12-01

    Blastocystis is a common unicellular eukaryotic parasite found not only in humans, but also in various kinds of animal species worldwide. Since Blastocystis isolates are morphologically indistinguishable, many molecular biological approaches have been applied to classify these isolates. The complete or partial sequences of the small subunit rRNA gene (SSU rDNA) are mainly used for comparisons and phylogenetic analyses among Blastocystis isolates. However, various lengths of the partial SSU rDNA sequence have been used for phylogenetic inference among genetically different isolates. Based on the complete SSU rDNA sequences, consensus terminology of nine subtypes (STs) of Blastocystis sp. that were supported by phylogenetically monophyletic nine clades was proposed in 2007. Thereafter, eight additional kinds of STs comprising non-human mammalian Blastocystis isolates have been reported based on the phylogeny of SSU rDNA sequences, while STs 11 and 12 were only proposed on the base of partial sequences. Although many sequence data from mammalian and avian Blastocystis are registered in GenBank, only limited data on SSU rDNA are available for poikilotherm-derived Blastocystis isolates. Therefore, the phylogenetic positions of the reptilian/amphibian Blastocystis clades are unstable. The phylogenetic inference of various STs comprising mammalian and/or avian Blastocystis isolates was verified herein based on comparisons between partial and complete SSU rDNA sequences, and the phylogenetic positions of reptilian and amphibian Blastocystis isolates were also investigated using 14 new Blastocystis isolates from reptiles with all known isolates from other reptilians, amphibians, and insects registered in GenBank.

  15. Location and pathogenic potential of Blastocystis in the porcine intestine.

    Science.gov (United States)

    Wang, Wenqi; Bielefeldt-Ohmann, Helle; Traub, Rebecca J; Cuttell, Leigh; Owen, Helen

    2014-01-01

    Blastocystis is an ubiquitous, enteric protozoan of humans and many other species. Human infection has been associated with gastrointestinal disease such as irritable bowel syndrome, however, this remains unproven. A relevant animal model is needed to investigate the pathogenesis/pathogenicity of Blastocystis. We concluded previously that pigs are likely natural hosts of Blastocystis with a potentially zoonotic, host-adapted subtype (ST), ST5, and may make suitable animal models. In this study, we aimed to characterise the host-agent interaction of Blastocystis and the pig, including localising Blastocystis in porcine intestine using microscopy, PCR and histopathological examination of tissues. Intestines from pigs in three different management systems, i.e., a commercial piggery, a small family farm and a research herd (where the animals were immunosuppressed) were examined. This design was used to determine if environment or immune status influences intestinal colonisation of Blastocystis as immunocompromised individuals may potentially be more susceptible to blastocystosis and development of associated clinical signs. Intestines from all 28 pigs were positive for Blastocystis with all pigs harbouring ST5. In addition, the farm pigs had mixed infections with STs 1 and/or 3. Blastocystis organisms/DNA were predominantly found in the large intestine but were also detected in the small intestine of the immunosuppressed and some of the farm pigs, suggesting that immunosuppression and/or husbandry factors may influence Blastocystis colonisation of the small intestine. No obvious pathology was observed in the histological sections. Blastocystis was present as vacuolar/granular forms and these were found within luminal material or in close proximity to epithelial cells, with no evidence of attachment or invasion. These results concur with most human studies, in which Blastocystis is predominantly found in the large intestine in the absence of significant organic

  16. Determination of recombination in Mycoplasma hominis

    DEFF Research Database (Denmark)

    Jacobsen, Iben Søgaard; Boesen, Thomas; Mygind, Tina;

    2002-01-01

    Mycoplasma hominis has been previously described as a heterogeneous species, and in the present study intraspecies diversity of 20 M. hominis isolates from different individuals was analyzed using parts of the unlinked gyrase B (gyrB), elongation factor Tu (tuf), SRalpha homolog (ftsY), hitB-hitL...

  17. Clinical significance and taxonomy of Actinobacillus hominis

    DEFF Research Database (Denmark)

    Friis-Møller, Alice; Christensen, J J; Fussing, V;

    2001-01-01

    Clinical findings in 36 immunosuppressed patients with lower respiratory tract infection or bacteremia with Actinobacillus hominis are described. Animal contact was only recorded for three patients; nine patients died despite appropriate antimicrobial treatment. Although infections with this micr...

  18. Blastocystis, an unrecognized parasite: an overview of pathogenesis and diagnosis

    OpenAIRE

    Wawrzyniak, Ivan; Poirier, Philippe; Viscogliosi, Eric; Dionigia, Meloni; Texier, Catherine; Delbac, Frédéric; Alaoui, Hicham El

    2013-01-01

    Blastocystis sp. is among the few enteric parasites with a prevalence that often exceeds 5% in the general population of industrialized countries and can reach 30–60% in developing countries. This parasite is frequently found in people who are immunocompromised (patients with human immunodeficiency virus/acquired immunodeficiency syndrome or cancer) and a higher risk of Blastocystis sp. infection has been found in people with close animal contact. Such prevalence in the human population and t...

  19. Blastocystis, an unrecognized parasite: an overview of pathogenesis and diagnosis.

    Science.gov (United States)

    Wawrzyniak, Ivan; Poirier, Philippe; Viscogliosi, Eric; Dionigia, Meloni; Texier, Catherine; Delbac, Frédéric; Alaoui, Hicham El

    2013-10-01

    Blastocystis sp. is among the few enteric parasites with a prevalence that often exceeds 5% in the general population of industrialized countries and can reach 30-60% in developing countries. This parasite is frequently found in people who are immunocompromised (patients with human immunodeficiency virus/acquired immunodeficiency syndrome or cancer) and a higher risk of Blastocystis sp. infection has been found in people with close animal contact. Such prevalence in the human population and the zoonotic potential naturally raise questions about the impact of these parasites on public health and has increased interest in this area. Recent in vitro and in vivo studies have shed new light on the pathogenic power of this parasite, suggesting that Blastocystis sp. infection is associated with a variety of gastrointestinal disorders, may play a significant role in irritable bowel syndrome, and may be linked with cutaneous lesions (urticaria). Despite recent significant advances in the knowledge of the extensive genetic diversity of this species, the identification of extracellular proteases as virulence factors and the publication of one isolate genome, many aspects of the biology of Blastocystis sp. remain poorly investigated. In this review, we investigate several biological aspects of Blastocystis sp. (diversity and epidemiology, diagnosis tools and pathophysiology). These data pave the way for the following challenges concerning Blastocystis sp. research: deciphering key biological mechanisms and pathways of this parasite and clarification of its clinical impact in humans.

  20. Genetic diversity of human blastocystis isolates in khorramabad, central iran.

    Directory of Open Access Journals (Sweden)

    Ebrahim Badparva

    2014-03-01

    Full Text Available There are some genetic differences in Blastocystis that show the existence of species or genotypes. One of these genes that help in identifying Blastocystis is SSUrRNA. The aim of this study was assessment of genetic diversity of Blastocystis by PCR with seven pairs of STS primers.This study was done on 511 stool samples collected from patients referred to the health care centers of Khorramabad, Central Iran, in 2012. Genomic DNA was extracted and in order to determine the Blastocystis subtype in contaminated samples, seven pairs of primers STS (subtype specific sequence-tagged site were used.Out of 511 samples, 33 (6.5% samples were infected with Blastocystis. Subtype (ST of 30 samples was identified and three subtypes 2, 3 and 4 were determined. Mix infection was reported 10% which 3.33% of the infection was for the mixture of ST 3 and ST5 and 6.67% was for the mixture of ST 2 and ST 3.The predominant subtype was ST3 that is the main human subtype. The dominance of ST2 and 5 are important in this study. This superiority has been reported in some of the studies in ST 2 which is different from the studies in other countries, because they have announced priorities of the ST1 and ST6 after ST3.

  1. Molecular design of Mycoplasma hominis Vaa adhesin

    DEFF Research Database (Denmark)

    Boesen, Thomas; Fedosova, Natalya U.; Kjeldgaard, Morten

    2001-01-01

    The variable adherence-associated (Vaa) adhesin of the opportunistic human pathogen Mycoplasma hominis is a surface-exposed, membrane-associated protein involved in the attachment of the bacterium to host cells. The molecular masses of recombinant 1 and 2 cassette forms of the protein determined...

  2. Dermatobia hominis infestation: a case report.

    Science.gov (United States)

    Lowry, M A; McEvoy, P L

    1992-12-01

    The hazards presented by the Central American tropical environment are myriad. We report a case of cutaneous myiasis caused by the human botfly, Dermatobia hominis, in a soldier who had participated in military operations in Central America. The clinical presentation, treatment, and unique life cycle of the human botfly is discussed.

  3. Homo homini : [luuletused] / Valeria Ränik

    Index Scriptorium Estoniae

    Ränik, Valeria, 1964-

    2004-01-01

    Sisu: Homo homini ; "Tegelikult on täiuslik kõik..." ; Mõned kõned ; "Vihmadest lekib lagi..." ; "Sündisid siia, et maksta maksu..." ; "Mingis kohas, mingil ajal..." ; "Emajõgi, Amme jõgi..." ; Kaktus ; Eraelamus ; "Minevikule vesi peale..."

  4. Geographic distribution of human Blastocystis subtypes in South America.

    Science.gov (United States)

    Ramírez, Juan David; Sánchez, Angie; Hernández, Carolina; Flórez, Carolina; Bernal, María Consuelo; Giraldo, Julio Cesar; Reyes, Patricia; López, Myriam Consuelo; García, Lineth; Cooper, Philip J; Vicuña, Yosselin; Mongi, Florencia; Casero, Rodolfo D

    2016-07-01

    Blastocystis is a cosmopolitan enteric protist colonizing probably more than 1 billion people. This protozoan exhibits genetic diversity and is subdivided into subtypes (STs). The aim of this study was to determine the distribution of Blastocystis STs in symptomatic and asymptomatic human samples from different countries of South America. A total of 346 fecal samples were genotyped by SSU rDNA showing ST1 (28.3%), ST2 (22.2%), ST3 (36.7%), ST4 (2%), ST5 (2.3%), ST6 (2%), ST7 (2.3%), ST8 (0.6%), ST12 (0.9%) and a novel ST (2.7%). These findings update the epidemiology of Blastocystis in South America and expand our knowledge of the phylogeographic differences exhibited by this stramenopile.

  5. Identification of Blastocystis subtype 1 variants in the Home for Girls, Bangkok, Thailand.

    Science.gov (United States)

    Thathaisong, Umaporn; Siripattanapipong, Suradej; Mungthin, Mathirut; Pipatsatitpong, Duangnate; Tan-ariya, Peerapan; Naaglor, Tawee; Leelayoova, Saovanee

    2013-02-01

    A cross-sectional study of Blastocystis infection was conducted to evaluate the prevalence, risk factors, and subtypes of Blastocystis at the Home for Girls, Bangkok, Thailand in November 2008. Of 370 stool samples, 118 (31.9%) were infected with Blastocystis. Genotypic characterization of Blastocystis was performed by polymerase chain reaction and sequence analysis of the partial small subunit ribosomal RNA (SSU rRNA) gene. Subtype 1 was the most predominant (94.8%), followed by subtype 6 (3.5%) and subtype 2 (1.7%). Sequence analyses revealed nucleotide polymorphisms for Blastocystis subtype 1, which were described as subtype 1/variant 1, subtype 1/variant 2. Blastocystis subtype 1/variant 1 was the most predominant infection occurring in almost every house. The results showed that subtype analysis of Blastocystis was useful for molecular epidemiological study.

  6. Cardiobacterium hominis-induced acute dacryocystitis and lacrimal abscess

    Directory of Open Access Journals (Sweden)

    Guru Prasad Manderwad

    2014-01-01

    Full Text Available Cardiobacterium hominis is a member of the HACEK (Haemophilus sp., Actinobacillus actinomycetemcomitans, C. hominis, Eikenella corrodens, and Kingella kingae group commonly associated with endocarditits and is normally present in the respiratory tract. We describe the first case of acute dacryocystitis with lacrimal abscess caused by C. hominis along with a brief review of the literature. The patient responded to oral and topical ciprofloxacin after incision and drainage and awaits dacryocystorhinostomy.

  7. Organelles in Blastocystis that Blur the Distinction between Mitochondria and Hydrogenosomes.

    OpenAIRE

    Stechmann, A; Hamblin, K; Perez-Brocal, V; Gaston, D; Richmond, GS; Giezen, M.; Clark, CG; Roger, AJ

    2008-01-01

    Summary Blastocystis is a unicellular stramenopile of controversial pathogenicity in humans [1, 2]. Although it is a strict anaerobe, Blastocystis has mitochondrion-like organelles with cristae, a transmembrane potential and DNA [2–4]. An apparent lack of several typical mitochondrial pathways has led some to suggest that these organelles might be hydrogenosomes, anaerobic organelles related to mitochondria [5, 6]. We generated 12,767 expressed sequence tags (ESTs) from Blastocystis and ident...

  8. Localization and nucleotide specificity of Blastocystis succinyl-CoA synthetase

    OpenAIRE

    Hamblin, Karleigh; Standley, Daron M; Rogers, Matthew B; Stechmann, Alexandra; Andrew J. Roger; Maytum, Robin; Giezen, Mark van der

    2008-01-01

    The anaerobic lifestyle of the intestinal parasite Blastocystis raises questions about the biochemistry and function of its mitochondria-like organelles. We have characterized the Blastocystis succinyl-CoA synthetase (SCS), a tricarboxylic acid cycle enzyme that conserves energy by substrate-level phosphorylation. We show that SCS localizes to the enigmatic Blastocystis organelles, indicating that these organelles might play a similar role in energy metabolism as classic mitochondria. Althoug...

  9. Molecular characterization of Blastocystis isolates from zoo animals and their animal-keepers.

    Science.gov (United States)

    Parkar, Unaiza; Traub, Rebecca J; Vitali, Simone; Elliot, Aileen; Levecke, Bruno; Robertson, Ian; Geurden, Thomas; Steele, Jan; Drake, Bev; Thompson, R C Andrew

    2010-04-19

    Blastocystis is an enteric protist and one of the most frequently reported parasitic infections in humans and a variety of animal hosts. It has also been reported in numerous parasite surveys of animals in zoological gardens and in particular in non-human primate species. PCR-based methods capable of the direct detection of Blastocystis in faeces were used to detect Blastocystis from various hosts, including non-human primates, Australian native fauna, elephants and giraffes, as well as their keepers from a Western Australian zoo. Additional faecal samples were also collected from elephants and giraffes from four other zoos in Amsterdam (The Netherlands), Antwerp (Belgium), Melbourne and Werribee (Australia). Information regarding the general health and lifestyle of the human volunteers were obtained by questionnaire. Overall, 42% and 63% of animals and zoo-keepers sampled from the Western Australian zoo were positive for Blastocystis, respectively. The occurrence of Blastocystis in elephants and giraffes from other cities was similar. This is the first report of Blastocystis found in the elephant, giraffe, quokka, southern hairy nosed wombat and western grey kangaroo. Three novel and what appear to be highly host-specific subtypes (STs) of Blastocystis in the elephant, giraffe and quokka are also described. These findings indicate that further exploration of the genetic diversity of Blastocystis is crucial. Most zoo-keepers at the Perth Zoo were harbouring Blastocystis. Four of these zoo-keeper isolates were identical to the isolates from the southern hairy nosed wombat and five primate species.

  10. The Mycoplasma hominis vaa gene displays a mosaic gene structure

    DEFF Research Database (Denmark)

    Boesen, Thomas; Emmersen, Jeppe M. G.; Jensen, Lise T.;

    1998-01-01

    Mycoplasma hominis contains a variable adherence-associated (vaa) gene. To classify variants of the vaa genes, we examined 42 M. hominis isolated by PCR, DNA sequencing and immunoblotting. This uncovered the existence of five gene categories. Comparison of the gene types revealed a modular compos...

  11. A retrospective metagenomics approach to studying Blastocystis

    DEFF Research Database (Denmark)

    Andersen, Lee O'Brien; Bonde, Ida; Nielsen, Henrik Bjørn

    2015-01-01

    a selection of 316 human faecal samples, hence representing genes originating from a single subtype. The 316 faecal samples were from 236 healthy individuals, 13 patients with Crohn's disease (CD) and 67 patients with ulcerative colitis (UC). The prevalence of Blastocystis was 20.3% in the healthy individuals...... metagenomic datasets targeting the bacterial component of the intestinal microbiome and the interplay between these microbial communities....

  12. Blastocystis sp. and other intestinal parasites in hemodialysis patients

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    Rose Anne Kulik

    2008-08-01

    Full Text Available Chronic renal insufficiency disease (CRI leads to uremia in hemodialysis patients and induces a state of immunodepression that results in higher frequencies of infections and diarrhea. Hemodialysis patients resident in the city of Campo Mourão, Paraná, Brazil were analyzed from April 2006 through September 2007 for Blastocystis sp. and other intestinal parasites and for associated diarrhea. Fecal samples from 86 hemodialysis patients and 146 healthy (reference persons were examined by standard methods for detecting ova, larvae and cysts, which included preservation in 10% formalin and the Kinyoun method. Thirty-three hemodialysis patients (45.1% and 36 reference individuals (25.7% were found to be parasitized. The differences in the percentages of parasitism and polyparasitism between the reference group and the chronic renal patients was significant (p= 0.0318 and 0.0019, respectively. Blastocystis sp. (18%-20.1%, Endolimax nana (14%-16.3%, Cryptosporidium sp. (4%-4.7% and Entamoeba coli (4%-4.7% were the most frequent protozoa found in the hemodialysis patients. Parasitism was not significantly associated with diarrhea (p=0.9947 or with decreased white blood cell counts (p=0.7046 in these individuals. Because parasitic infections may be an important comorbidity factor in hemodialysis patients, we suggest that parasitological stool examinations, especially for Blastocystis sp. and Cryptosporidium sp., be included in routine medical follow-up examinations of these patients.

  13. Draft genome sequence of the intestinal parasite Blastocystis subtype 4-isolate WR1

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    Ivan Wawrzyniak

    2015-06-01

    Full Text Available The intestinal protistan parasite Blastocystis is characterized by an extensive genetic variability with 17 subtypes (ST1–ST17 described to date. Only the whole genome of a human ST7 isolate was previously sequenced. Here we report the draft genome sequence of Blastocystis ST4-WR1 isolated from a laboratory rodent at Singapore.

  14. Oh my aching gut: irritable bowel syndrome, Blastocystis, and asymptomatic infection

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    Zhou Xiao-Nong

    2008-10-01

    Full Text Available Abstract Blastocystis is a prevalent enteric protozoan that infects a variety of vertebrates. Infection with Blastocystis in humans has been associated with abdominal pain, diarrhea, constipation, fatigue, skin rash, and other symptoms. Researchers using different methods and examining different patient groups have reported asymptomatic infection, acute symptomatic infection, and chronic symptomatic infection. The variation in accounts has lead to disagreements concerning the role of Blastocystis in human disease, and the importance of treating it. A better understanding of the number of species of Blastocystis that can infect humans, along with realization of the limitations of the existing clinical laboratory diagnostic techniques may account for much of the disagreement. The possibility that disagreement was caused by the emergence of particular pathogenic variants of Blastocystis is discussed, along with the potential role of Blastocystis infection in irritable bowel syndrome (IBS. Findings are discussed concerning the role of protease-activated receptor-2 in enteric disease which may account for the presence of abdominal pain and diffuse symptoms in Blastocystis infection, even in the absence of fever and endoscopic findings. The availability of better diagnostic techniques and treatments for Blastocystis infection may be of value in understanding chronic gastrointestinal illness of unknown etiology.

  15. Characterization of two cysteine proteases secreted by Blastocystis ST7, a human intestinal parasite.

    Science.gov (United States)

    Wawrzyniak, Ivan; Texier, Catherine; Poirier, Philippe; Viscogliosi, Eric; Tan, Kevin S W; Delbac, Frédéric; El Alaoui, Hicham

    2012-09-01

    Blastocystis spp. are unicellular anaerobic intestinal parasites of both humans and animals and the most prevalent ones found in human stool samples. Their association with various gastrointestinal disorders raises the questions of its pathogenicity and of the molecular mechanisms involved. Since secreted proteases are well-known to be implicated in intestinal parasite virulence, we intended to determine whether Blastocystis spp. possess such pathogenic factors. In silico analysis of the Blastocystis subtype 7 (ST7) genome sequence highlighted 22 genes coding proteases which were predicted to be secreted. We characterized the proteolytic activities in the secretory products of Blastocystis ST7 using specific protease inhibitors. Two cysteine proteases, a cathepsin B and a legumain, were identified in the parasite culture supernatant by gelatin zymographic SDS-PAGE gel and MS/MS analysis. These proteases might act on intestinal cells and disturb gut function. This work provides serious molecular candidates to link Blastocystis spp. and intestinal disorders.

  16. Antimicrobial susceptibility and susceptibility testing of Mycoplasma hominis: a review.

    Science.gov (United States)

    Bygdeman, S M; Mårdh, P A

    1983-01-01

    The determination of the minimal growth-inhibiting concentration (MIC), the minimal metabolism-inhibiting concentration (MMC), and the minimal mycoplasmacidal concentration (MCC) of various antimicrobial compounds for Mycoplasma hominis is influenced by the pH of the test media, the inoculum size, and the incubation time, although each of these factors generally do not affect the minimal concentration more than fourfold. M. hominis is resistant to beta-lactam antibiotics, vancomycin, sulfonamides, trimethoprim, and polymyxin B. There are great differences in the susceptibility of M. hominis to various macrolide antibiotics. Thus the organism is resistant to erythromycin and oleandomycin, moderately resistant to tylosin and spiramycin, susceptible to josamycin as well as to another macrolide drug, labelled M-4365G. M. hominis is also highly susceptible to the macrolide-like compound rosaramicin and to the tetracyclines (although resistant strains occur). It is susceptible to lincomycin and clindamycin, and moderately susceptible to chloramphenicol and rifampicin. The aminoglycosides have limited activity against M. hominis.

  17. Serological differentiation of microsporidia with special reference to Trachipleistophora hominis

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    Cheney S.A.

    2001-06-01

    Full Text Available Myositis is a common clinical syndrome in advanced stages of AIDS. Trachipleistophora hominis (phylum Microspora has been detected in several cases of painful, immobilising myositis in AIDS patients. Enzyme linked immunosorbent assays (ELISAs and Western blotting of protein profiles separated by SDS PAGE were used to determine whether this species could be detected and differentiated by serology. Sixteen microsporidia, including several species known to infect man and species infecting fish, crustaceans and a mosquito, were used as antigen. Each species had a unique profile of SDS PAGE-separated proteins. In Western blots, mouse antiserum, raised to T. hominis and selected for its high ELISA specificity, bound to antigens ranging from less than 25 kDa to greater than 250 kDa with major bands at 39-44 kDa and 98-150 kDa on T. hominis protein profiles. The serum also recognised some high molecular weight antigens in the profiles of Vavraia culicis, Heterosporis anguillarum, and three species of Pleistophora but none in the remaining genera examined. It was concluded that ELISA and Western blotting could be used to detect and differentiate T. hominis in muscle biopsy tissue from patients with myositis. However, sera from T. hominis infected patients in the terminal stages of AIDS would not be useful for detection of infections because of a sharp decline in antibody level.

  18. Cardiobacterium hominis endocarditis: A case report and review of the literature

    OpenAIRE

    Andrew Walkty

    2005-01-01

    The present case report describes the clinical course of a patient who presented with Cardiobacterium hominis endocarditis. A review of the literature follows the case presentation. C hominis, a fastidious Gram-negative bacillus, is a member of the HACEK group of microorganisms (Haemophilus species, Actinobacillus actinomycetemcomitans, C hominis, Eikenella corrodens and Kingella kingae). Endocarditis caused by C hominis is uncommon and generally follows a subacute course. Patients may presen...

  19. Epidemiologic Study of Blastocystis Infection in an Urban Community in the Philippines.

    Science.gov (United States)

    Belleza, Maria Luz B; Cadacio, Jessa Louise C; Borja, Maridel P; Solon, Juan Antonio A; Padilla, Mildred A; Tongol-Rivera, Pilarita N; Rivera, Windell L

    2015-01-01

    Blastocystis has been considered as the most common intestinal parasite in humans and has an augmented impact on public health. However, the prevalence of this parasite in the Philippines has not been determined. To contribute to a better understanding of the epidemiology of this infection, a cross-sectional study aimed at providing the first documented data on the prevalence and correlates, sociodemographic factors, hygiene practices, source of water supply, and dog ownership, associated with Blastocystis infection was carried out in randomly selected communities at Pateros, Metro Manila. Fecal samples from respondents were collected and cultured in diphasic agar medium for 3-7 days and examined using light microscopy. Of the 1,271 respondents, 12.98% (95% CI: 11.13-14.83) were detected positive for Blastocystis. Among the correlates of Blastocystis infection, dog ownership was found significantly associated as confirmed by multivariate analysis. Therefore, this factor should be considered in information to create awareness about Blastocystis and to prevent and control Blastocystis infection in particular and diarrheal diseases in general. Further studies using molecular approaches to distinguish subtype and to determine genetic characteristics of isolates from humans and dogs are recommended to analyze their relationship and provide more conclusive evidence of cross-transmission.

  20. Subtype analysis of Blastocystis sp. isolates from asymptomatic individuals in an urban community in the Philippines

    Science.gov (United States)

    Adao, Davin Edric V.; Dela Serna, Ace O.; Belleza, Maria Luz B.; Bolo, Nicole R.; Rivera, Windell L.

    2016-10-01

    Blastocystis sp. is a commonly reported enteric protistan parasite in faecal specimens with a worldwide distribution afflicting both humans and a wide range of animals. The aim of this study is to characterize the subtypes (STs) of Blastocystis sp. isolates from asymptomatic individuals in an urban community in Pateros, Metro Manila, Philippines. The 600-bp small subunit ribosomal RNA (SSU rRNA) barcoding region of Blastocystis sp. isolates was amplified and sequenced using the primers RD5 and BhRDr. Subtypes were identified by uploading the sequences onto the Basic Local Alignment and Search Tool (BLAST) websites, the Blastocystis Subtype (18S) and Sequence Typing (MLST) Database and by construction of a phylogenetic tree. Twenty-nine (29) out of 35 individuals were detected positive for Blastocystis sp. ST3 is the most common among the three STs detected (65.5%), followed by ST1 (31.0%) and ST4 (3.44%). This study showed that DNA barcoding can serve as a helpful tool to investigate the diversity of Blastocystis sp. in the Philippines.

  1. Epidemiologic Study of Blastocystis Infection in an Urban Community in the Philippines

    Directory of Open Access Journals (Sweden)

    Maria Luz B. Belleza

    2015-01-01

    Full Text Available Blastocystis has been considered as the most common intestinal parasite in humans and has an augmented impact on public health. However, the prevalence of this parasite in the Philippines has not been determined. To contribute to a better understanding of the epidemiology of this infection, a cross-sectional study aimed at providing the first documented data on the prevalence and correlates, sociodemographic factors, hygiene practices, source of water supply, and dog ownership, associated with Blastocystis infection was carried out in randomly selected communities at Pateros, Metro Manila. Fecal samples from respondents were collected and cultured in diphasic agar medium for 3–7 days and examined using light microscopy. Of the 1,271 respondents, 12.98% (95% CI: 11.13–14.83 were detected positive for Blastocystis. Among the correlates of Blastocystis infection, dog ownership was found significantly associated as confirmed by multivariate analysis. Therefore, this factor should be considered in information to create awareness about Blastocystis and to prevent and control Blastocystis infection in particular and diarrheal diseases in general. Further studies using molecular approaches to distinguish subtype and to determine genetic characteristics of isolates from humans and dogs are recommended to analyze their relationship and provide more conclusive evidence of cross-transmission.

  2. Furuncular myiasis caused by Dermatobia hominis, the human botfly.

    Science.gov (United States)

    Maier, Harald; Hönigsmann, Herbert

    2004-02-01

    Myiasis is a common travel-associated dermatosis. Travelers to many parts of Central and South America are susceptible to infestation by Dermatobia hominis. Despite the common name of human botfly, D hominis infests a broad range of mammals and is a severe pest to economically important farm animals in endemic regions. The adult female does not lay the eggs on the host. Instead, the adult female infests hosts indirectly by using blood-feeding arthropods to serve as phoretic vectors to transport the eggs. We present a patient who acquired Dermatobia when bitten by a day-active mosquito during a visit to Guatemala. He had a locally painful, firm furuncular lesion with a central pore that drained serosanguineous exudates. The patient applied an occlusive ointment and recovered the larva after it emerged. In this report we discuss the life cycle of D hominis, the differential diagnosis, and therapeutic approaches.

  3. Prevalence of blastocystis in shelter-resident and client-owned companion animals in the US Pacific Northwest.

    Science.gov (United States)

    Ruaux, Craig G; Stang, Bernadette V

    2014-01-01

    Domestic dogs and cats are commonly infected with a variety of protozoan enteric parasites, including Blastocystis spp. In addition, there is growing interest in Blastocystis as a potential enteric pathogen, and the possible role of domestic and in-contact animals as reservoirs for human infection. Domestic animals in shelter environments are commonly recognized to be at higher risk for carriage of enteropathogens. The purpose of this study was to determine the frequency of infection of shelter-resident and client-owned domestic dogs and cats with Blastocystis spp in the Pacific Northwest region of the USA. Fecal samples were collected from 103 shelter-resident dogs, 105 shelter-resident cats, 51 client-owned dogs and 52 client-owned cats. Blastocystis were detected and subtypes assigned using a nested PCR based on small subunit ribosomal DNA sequences. Shelter-resident animals were significantly more likely to test positive for Blastocystis (Panimals were carrying a variety of Blastocystis subtypes. No relationship was seen between Blastocystis carriage and the presence of gastrointestinal disease signs in either dogs or cats. These data suggest that, as previously reported for other enteric pathogens, shelter-resident companion animals are a higher risk for carriage of Blastocystis spp. The lack of relationship between Blastocystis carriage and intestinal disease in shelter-resident animals suggests that this organism is unlikely to be a major enteric pathogen in these species.

  4. Sterols of Pneumocystis carinii hominis organisms isolated from human lungs

    DEFF Research Database (Denmark)

    Kaneshiro, E S; Amit, Z; Chandra, Jan Suresh

    1999-01-01

    in conjunction with analyses of chemically synthesized authentic standards. The sterol composition of isolated P. carinii hominis organisms has yet to be reported. If P. carinii from animal models is to be used for identifying potential drug targets and for developing chemotherapeutic approaches to clear human...... infections, it is important to determine whether the 24-alkylsterols of organisms found in rats are also present in organisms in humans. In the present study, sterol analyses of P. carinii hominis organisms isolated from cryopreserved human P. carinii-infected lungs and from bronchoalveolar lavage fluid were...

  5. Complete Genome Sequence of the Human Gut Symbiont Roseburia hominis

    DEFF Research Database (Denmark)

    Travis, Anthony J.; Kelly, Denise; Flint, Harry J;

    2015-01-01

    We report here the complete genome sequence of the human gut symbiont Roseburia hominis A2-183(T) (= DSM 16839(T) = NCIMB 14029(T)), isolated from human feces. The genome is represented by a 3,592,125-bp chromosome with 3,405 coding sequences. A number of potential functions contributing to host-...

  6. Genomic and gene variation in Mycoplasma hominis strains

    DEFF Research Database (Denmark)

    Christiansen, Gunna; Andersen, H; Birkelund, Svend;

    1987-01-01

    DNAs from 14 strains of Mycoplasma hominis isolated from various habitats, including strain PG21, were analyzed for genomic heterogeneity. DNA-DNA filter hybridization values were from 51 to 91%. Restriction endonuclease digestion patterns, analyzed by agarose gel electrophoresis, revealed no ide...

  7. Inhibitory effect of Ferula asafoetida L. (Umbelliferae) on Blastocystis sp. subtype 3 growth in vitro.

    Science.gov (United States)

    El Deeb, Hala K; Al Khadrawy, Faisal M; Abd El-Hameid, Ayman K

    2012-09-01

    Asafoetida is an oleo-gum-resin obtained from many Ferula species and frequently used in traditional medicine. The current study aimed to evaluate the activity of asafoetida against the in vitro growth of Blastocystis sp. Asafoetida as powder-form (Ap) and oil-form (Ao) extracts at concentrations of 2, 4, 8, 16, and 20 mg/ml and 5, 10, 25, 40, and 50 mg/ml, respectively were incubated with isolates of Blastocystis sp. subtype 3 for 24, 72, and 144 h and compared to the reference antiprotozoan drug metronidazole at concentrations of 10, 100, and 500 μg/ml. Asafoetida either as Ap or Ao decreased counts and viability of all tested isolates of Blastocystis sp. subtype 3 which was confirmed by microscopy. The degree of the inhibitory effect was dependent on the concentration, form, as well as the time of incubation with asafoetida extracts. The lowest concentrations of Ap and Ao that caused complete (100 %) inhibition of Blastocystis growth and highest (100 %) percentage inhibition of multiplication was 16 and 40 mg/ml, respectively; mean counts at these concentrations either did not differ or decreased significantly when compared to metronidazole control (p < 0.05). Also, the parasites did not resume growth after re-cultivation in asafoetida-free medium when examined further after 48, 72, and 144 h of cultivation. These findings demonstrate the potential of phytomedicine asafoetida as a potent natural alternative for treatment of Blastocystis sp.infection.

  8. Subtype Distribution of Blastocystis in Communities along the Chao Phraya River, Thailand.

    Science.gov (United States)

    Palasuwan, Attakorn; Palasuwan, Duangdao; Mahittikorn, Aongart; Chiabchalard, Rachatawan; Combes, Valery; Popruk, Supaluk

    2016-08-01

    Blastocystis is a common zoonotic enteric protozoan that has been classified into 17 distinct subtypes (STs). A cross-sectional study was conducted to determine the prevalence and subtype distributions of Blastocystis in villagers living along the Chao Phraya River, Ayutthaya Province, Thailand, and to assess the risk of zoonotic infection. In total, 220 stool samples were collected, and DNA was extracted. PCR and sequencing were performed with primers targeting the small-subunit ribosomal RNA (SSU rRNA) genes. Blastocystis was present in 5.9% (13/220) of samples, and ST3 (5.0%; 11/220) was the predominant subtype, followed by ST2 (0.45%; 1/220) and ST6 (0.45%; 1/220). Phylogenetic trees were constructed with the maximum-likelihood method based on the Hasegawa-Kishino-Yano + G + I model, neighbor-joining, and maximum parsimony methods. The percentage of bootstrapped trees in which the associated taxa clustered together was relatively high. All the sequences of the Blastocystis-positive samples (KU051524-KU051536) were closely related to those from animals (pig, cattle, and chicken), indicating a zoonotic risk. Therefore, the villagers require proper health education, especially regarding the prevention of parasitic infection, to improve their personal hygiene and community health. Further studies are required to investigate the Blastocystis STs in the animals living in these villages.

  9. Prevalence and Risk Factors for Blastocystis Infection Among Children and Caregivers in a Child Care Center, Bangkok, Thailand.

    Science.gov (United States)

    Pipatsatitpong, Duangnate; Leelayoova, Saovanee; Mungthin, Mathirut; Aunpad, Ratchaneewan; Naaglor, Tawee; Rangsin, Ram

    2015-08-01

    In September 2009, a cross-sectional study was conducted to evaluate parasitic infections in a child care center in Khlong Toei, Bangkok, Thailand. Of 503 children and staff members, 258 (51.3%) stool samples and questionnaires were obtained. The most common parasitic infection was Blastocystis sp. (13.6%). Blastocystis sp. subtype 3 was predominantly found (80.0%), followed by subtypes 2 (12.0%) and 1 (8.0%). The prevalence of Blastocystis infection varied among different age groups. The prevalence of Blastocystis infection in non-HIV-infected children aged < 10 and 10-19 years were 14.5% and 10.3%, respectively, which were not significantly different. All 31 HIV-infected children were not infected with Blastocystis sp. The most likely reason could be the result of properly using prevention measures for this specific group.

  10. Microbiological and molecular characterization of Staphylococcus hominis isolates from blood.

    Directory of Open Access Journals (Sweden)

    Soraya Mendoza-Olazarán

    Full Text Available BACKGROUND: Among Coagulase-Negative Staphylococci (CoNS, Staphylococcus hominis represents the third most common organism recoverable from the blood of immunocompromised patients. The aim of this study was to characterize biofilm formation, antibiotic resistance, define the SCCmec (Staphylococcal Chromosomal Cassette mec type, and genetic relatedness of clinical S. hominis isolates. METHODOLOGY: S. hominis blood isolates (n = 21 were screened for biofilm formation using crystal violet staining. Methicillin resistance was evaluated using the cefoxitin disk test and the mecA gene was detected by PCR. Antibiotic resistance was determined by the broth microdilution method. Genetic relatedness was determined by pulsed-field gel electrophoresis (PFGE and SCCmec typed by multiplex PCR using two different methodologies described for Staphylococcus aureus. RESULTS: Of the S. hominis isolates screened, 47.6% (10/21 were categorized as strong biofilm producers and 23.8% (5/21 as weak producers. Furthermore, 81% (17/21 of the isolates were methicillin resistant and mecA gene carriers. Resistance to ampicillin, erythromycin, and trimethoprim was observed in >70% of isolates screened. Each isolate showed a different PFGE macrorestriction pattern with similarity ranging between 0-95%. Among mecA-positive isolates, 14 (82% harbored a non-typeable SCCmec type: eight isolates were not positive for any ccr complex; four contained the mec complex A ccrAB1 and ccrC, one isolate contained mec complex A, ccrAB4 and ccrC, and one isolate contained the mec complex A, ccrAB1, ccrAB4, and ccrC. Two isolates harbored the association: mec complex A and ccrAB1. Only one strain was typeable as SCCmec III. CONCLUSIONS: The S. hominis isolates analyzed were variable biofilm producers had a high prevalence of methicillin resistance and resistance to other antibiotics, and high genetic diversity. The results of this study strongly suggested that S. hominis isolates harbor

  11. Blastocystis and urticaria: Examination of subtypes and morphotypes in an unusual clinical manifestation.

    Science.gov (United States)

    Casero, Rodolfo Daniel; Mongi, Florencia; Sánchez, Angie; Ramírez, Juan David

    2015-08-01

    Blastocystis is a human common enteric protist that may colonize a large variety of non-human hosts linked to symptoms and diseases such as abdominal pain, constipation, diarrhea, urticaria, flatulence and irritable bowel syndrome (IBS). Blastocystis exhibits remarkable genetic diversity and multiple subtypes (STs) within the genus with no absolute associations with clinical symptomatology. Here we analyzed fecal samples from Argentinean patients (n=270) belonging to symptomatic (urticaria and non-specific gastrointestinal symptoms, n=39) and asymptomatic control (n=28). Those patients infected with Blastocystis (n=67) were submitted for morphological analysis, DNA extraction, 18S PCR, sequencing and STs identification according to DNA barcoding. Blastocystis vacuolar forms were the predominant morphotype (75%), ameboid-like forms were evidenced in 1.5% of samples. Blastocystis ST3 was detected in 71.6% (n=48), of which 71.4%, (n=35) and 28.6% (n=14) belonged to symptomatic and asymptomatic respectively. Other subtypes identified were ST1 (14.9%), ST6 (7.5%) and ST2 (5.9%). Blastocystis 18S barcoding evidenced in non-urticaria symptomatic patients and asymptomatic control group the presence of allele 134 (ST3) (p<0.0001), while allele 34 (ST3) was detected in 85.7% (18/21) of symptomatic uricaria as compared with control group (1/21) (p<0.0001). The presence of a particular allele (a34) significantly associated with urticaria patients was detected and the clinical implications of these findings are herein discussed.

  12. Blastocystis subtyping and its association with intestinal parasites in children from different geographical regions of Colombia

    Science.gov (United States)

    Flórez, Carolina; Olivera, Mario; Bernal, María Consuelo; Giraldo, Julio Cesar

    2017-01-01

    Blastocystis is a common enteric protist colonizing probably more than 1 billion people with a large variety of non-human hosts. Remarkable genetic diversity has been observed, leading to the subdivision of the genus into multiple subtypes (ST), some of which are exclusively found in non-human hosts. The aim of this study was to determine the distribution of Blastocystis STs/18S alleles in symptomatic (abdominal pain, anal pruritus, diarrhea, headache, nauseas and/or vomit) and asymptomatic children from nine geographical regions of Colombia. A total of 2026 fecal samples were collected as part of a national survey to estimate the frequency of intestinal parasites in children. A set of 256 samples that were Blastocystis positive was finally selected. The samples were submitted to DNA extraction, Real Time PCR and sequencing using Blastocystis-specific primers targeting the small subunit rRNA gene for ST identification. DNA of Ascaris lumbricoides (16.4%), Trichuris trichiura (8.2%), hookworms (Necator americanus/Ancylostoma duodenale) (7.3%), Giardia duodenalis (23.1%), Entamoeba complex (82%), Entamoeba coli (55%), Hymenolepis nana (0.8%), Endolimax nana (33.2%) and Neobalantidium coli (2.7%) was detected in the Blastocystis-positive samples. We detected ST1 (21.4%), ST2 (19.5%), ST3 (55.5%), ST4 (0.8%), ST6 (2%) and ST7 (0.8%); alleles 1, 2, 4, 81, 82 and 83 for ST1; alleles 9, 11, 12, 15, 67, 71 and 73 for ST2; alleles 34, 36, 38, 45, 49, 55, 134 and 128 for ST3; allele 42 for ST4; allele 122 for ST6, and allele 142 for ST7. Further studies implementing high-resolution molecular markers are necessary to understand the dynamics of Blastocystis transmission and the role of this Stramenopila in health and disease. PMID:28222192

  13. Presence of an Active Efflux System in the Fluoroquinolones Resistance of Mycoplasma Hominis

    Institute of Scientific and Technical Information of China (English)

    姚艳冰; 吴移谋; 朱翠明; 曾铁兵; 曾焱华

    2003-01-01

    Objective: To investigate the possible presence of an active efflux system in resistance to fluoroqninolones in Mycoplasma hominis. Methods: The resistant strains of M. hominis were selected from one hundred and three clinical strains of M. homlnls by broth microdilution method. The ac-cumulation of ciprofloxacin in M. hominis and the in-fluence of carbonyl cyanide m-chlorophenyl- hydrazone (CCCP) and reserpine were measured by a fluores-cence method. Results: Two resistant strains and two susceptible strains of M. hominis were selected in vitro. The accu-mulation of ciprofloxacin for resistant strains is lower than that of susceptible strains. CCCP and reserpine had different influence on clinical strains of M.hominis. Reserpine could dramatically increase the accumulation of ciprofloxacin, however CCCP had a little effect on it. Conclusion: These results suggest that the pres-ence of an active efflux system implicated in the fluoroouinolones-resistant in M. hominis.

  14. The Effect of Hominis Placenta Herbal Acupuncture on Bell's palsy

    OpenAIRE

    2000-01-01

    This report was done to observe the effect of Hominis placenta herbal acupuncture on Bell's palsy. The study group comprised 16 patients who arrived at Woo-suk university oriental hospital from January, 1999 till January, 2000 for Bell's palsy. All patients were divided into two group. One was herbal acupunture group, and the other was control group. Acupunture group was done herbal acupuncture therapy on the facial acupuncture points. Followings are achievement and a term of each group. I...

  15. A Preliminary Study on Preservation Methods of Blastocystis hominis%人芽囊原虫保存方法的初步研究

    Institute of Scientific and Technical Information of China (English)

    田春林; 万孝玲; 刘登宇; 何登贤

    2007-01-01

    目的 观察冻存剂、温度等因素对人芽囊原虫活力的影响,探索理想的人芽囊原虫保存方法.方法 从患者阳性粪便中分离人芽囊原虫,分装到2 ml无菌冻存管内,分别加入10%二甲基亚砜(dimethylsulfoxide,DMS0)、40%丙三醇(GL)和15%乙二醇(EG)作冻存剂,放置于不同的温度下保存,用台盼蓝染色法和原虫培养法测定细胞的活力和增殖能力.结果 虫体在室温(18℃~20℃)下可存活3周,在4℃~6℃存活不到1周.加入冻存剂后,置-20℃和液氮(-196℃)下可存活3个月以上.用40%GL作冻存剂,于液氮低温下冻存的虫体保存半年后复苏,活力仍达41.7%,培养72 h后多见分裂相细胞.结论 应用40%GL作为冻存剂,在液氮中低温保存人芽囊原虫效果较佳.4℃不适于保存人芽囊原虫.

  16. Prevalence of blastocystis in shelter-resident and client-owned companion animals in the US Pacific Northwest.

    Directory of Open Access Journals (Sweden)

    Craig G Ruaux

    Full Text Available Domestic dogs and cats are commonly infected with a variety of protozoan enteric parasites, including Blastocystis spp. In addition, there is growing interest in Blastocystis as a potential enteric pathogen, and the possible role of domestic and in-contact animals as reservoirs for human infection. Domestic animals in shelter environments are commonly recognized to be at higher risk for carriage of enteropathogens. The purpose of this study was to determine the frequency of infection of shelter-resident and client-owned domestic dogs and cats with Blastocystis spp in the Pacific Northwest region of the USA. Fecal samples were collected from 103 shelter-resident dogs, 105 shelter-resident cats, 51 client-owned dogs and 52 client-owned cats. Blastocystis were detected and subtypes assigned using a nested PCR based on small subunit ribosomal DNA sequences. Shelter-resident animals were significantly more likely to test positive for Blastocystis (P<0.05 for dogs, P = 0.009 for cats. Sequence analysis indicated that shelter-resident animals were carrying a variety of Blastocystis subtypes. No relationship was seen between Blastocystis carriage and the presence of gastrointestinal disease signs in either dogs or cats. These data suggest that, as previously reported for other enteric pathogens, shelter-resident companion animals are a higher risk for carriage of Blastocystis spp. The lack of relationship between Blastocystis carriage and intestinal disease in shelter-resident animals suggests that this organism is unlikely to be a major enteric pathogen in these species.

  17. Prevalence and subtype distribution of Blastocystis in healthy individuals in Sharjah, United Arab Emirates.

    Science.gov (United States)

    AbuOdeh, Raed; Ezzedine, Sinda; Samie, Amidou; Stensvold, Christen Rune; ElBakri, Ali

    2016-01-01

    Blastocystis is estimated to be one of the most common parasites of the intestinal tract of humans, comprising multiple subtypes (ST). Meanwhile, the distribution of Blastocystis ST in many communities and countries remains unknown. In the present work, we aimed to identify the prevalence of Blastocystis and the ST distribution in human stool samples collected from healthy expatriates from different geographical regions and residing in Sharjah, United Arabian Emirates (UAE). A total of 133 samples were screened and subtyped using partial small subunit ribosomal RNA gene sequencing. Fifty-nine (44.4%) samples were identified as positive. Among these, 39 were successfully sequenced and subtyped. The ST distribution was as follows: ST3, 58.9% (23/39); ST1, 28.2% (11/39); and ST2, 7.6% (3/39). No correlation between geographic origin and infection (χ(2)=11.006; P=0.528) nor gender and infection (χ(2)=1.264; P=0.261) was observed. The data were compared with those available for other Middle Eastern and North African neighboring countries. This study is the first to provide data concerning the prevalence of Blastocystis and the frequency of various STs in the UAE, confirming the absence of ST4 and the commonness of ST1, ST2, and ST3 in this geographical region.

  18. Biochemical characterization of a mitochondrial-like organelle from Blastocystis sp. subtype 7.

    Science.gov (United States)

    Lantsman, Yelena; Tan, Kevin S W; Morada, Mary; Yarlett, Nigel

    2008-09-01

    A mitochondrion-like organelle (MLO) was isolated from isotonic homogenates of Blastocystis. The organelle sedimented at 5000 g for 10 min, and had an isopycnic density in sucrose of 1.2 g ml(-1). Biochemical characterization enabled the demonstration of several key enzymes that allowed the construction of a metabolic pathway consisting of an incomplete Krebs cycle linked to the oxygen-sensitive enzymes pyruvate : NADP(+) oxidoreductase (PNO), acetate : succinate CoA transferase (ASCT) and succinate thiokinase (STK), which cumulatively are responsible for recycling CoA and generating ATP. The organelle differs from typical aerobic mitochondria in possessing an oxygen-sensitive PNO that can use FAD(+) or FMN(+) as electron acceptor but is inactive with NAD(+), Spinacia oleracea ferredoxin or Clostridium pasteurianum ferredoxin. A gene with 77 % sequence similarity to the PNO mitochondrion precursor cluster from Euglena gracilis sp[Q941N5] was identified in the Blastocystis genome database. A second cluster with 56 % sequence similarity to the pyruvate : ferredoxin oxidoreductase (PFOR) from Trichomonas vaginalis was also identified, which is in agreement with the concept that the PNO gene arose through the fusion of a eubacterial gene for PFOR with the gene for NADPH : cytochrome p450 reductase. Hydrogenase activity was not detected under the conditions used in this study. The Blastocystis oranelle therefore demonstrates significant biochemical differences from traditional mitochondria and hydrogenosomes, but possesses features of both. Based upon the results of this study, the Blastocystis organelle falls into the category of a MLO.

  19. Organelles in Blastocystis that blur the distinction between mitochondria and hydrogenosomes.

    Science.gov (United States)

    Stechmann, Alexandra; Hamblin, Karleigh; Pérez-Brocal, Vicente; Gaston, Daniel; Richmond, Gregory S; van der Giezen, Mark; Clark, C Graham; Roger, Andrew J

    2008-04-22

    Blastocystis is a unicellular stramenopile of controversial pathogenicity in humans. Although it is a strict anaerobe, Blastocystis has mitochondrion-like organelles with cristae, a transmembrane potential and DNA. An apparent lack of several typical mitochondrial pathways has led some to suggest that these organelles might be hydrogenosomes, anaerobic organelles related to mitochondria. We generated 12,767 expressed sequence tags (ESTs) from Blastocystis and identified 115 clusters that encode putative mitochondrial and hydrogenosomal proteins. Among these is the canonical hydrogenosomal protein iron-only [FeFe] hydrogenase that we show localizes to the organelles. The organelles also have mitochondrial characteristics, including pathways for amino acid metabolism, iron-sulfur cluster biogenesis, and an incomplete tricarboxylic acid cycle as well as a mitochondrial genome. Although complexes I and II of the electron transport chain (ETC) are present, we found no evidence for complexes III and IV or F1Fo ATPases. The Blastocystis organelles have metabolic properties of aerobic and anaerobic mitochondria and of hydrogenosomes. They are convergently similar to organelles recently described in the unrelated ciliate Nyctotherus ovalis. These findings blur the boundaries between mitochondria, hydrogenosomes, and mitosomes, as currently defined, underscoring the disparate selective forces that shape these organelles in eukaryotes.

  20. Cutaneous myiasis due to Dermatobia hominis: a report of six cases.

    Science.gov (United States)

    Gordon, P M; Hepburn, N C; Williams, A E; Bunney, M H

    1995-05-01

    We report six cases of Dermatobia hominis myiasis imported into the U.K. from Belize. With increasing international travel, myiasis may be encountered more frequently in countries in which the parasites are not indigenous. The life-cycle of D. hominis is described, and scanning electron micrographs show the detailed appearance of the larva.

  1. An epidemiological survey of Mycoplasma hominis and Ureaplasma urealyticum in gynaecological outpatients, Rome, Italy.

    Science.gov (United States)

    Verteramo, R; Patella, A; Calzolari, E; Recine, N; Marcone, V; Osborn, J; Chiarini, F; Degener, A M

    2013-12-01

    The objective of this study was to assess the prevalence of Ureaplasma urealyticum and Mycoplasma hominis infections and to investigate associations between their presence in the lower female genital tract and lifestyle characteristics. The study was performed on a population of 3115 women, comparing the demographic and behavioural characteristics of 872 women with U. urealyticum infection and 142 women with M. hominis with uninfected women, using univariate and multiple logistic regression analysis. The prevalence of infection with U. urealyticum was 28% and M. hominis was 4.6%. In multivariate logistic regression analysis, intrauterine device, number of sexual partners and age (<35 years) were significantly associated with U. urealyticum while previous induced abortion, condom use and young age at first intercourse (<16 years) were associated with M. hominis infection. U. urealyticum infection presents the same demographic and behavioural characteristics of a sexually transmitted disease. The unprotective role of condom use suggests a non-sexual mode of transmission of M. hominis infection.

  2. Prevalence of Blastocystis in Shelter-Resident and Client-Owned Companion Animals in the US Pacific Northwest

    OpenAIRE

    Ruaux, Craig G.; Stang, Bernadette V

    2014-01-01

    Domestic dogs and cats are commonly infected with a variety of protozoan enteric parasites, including Blastocystis spp. In addition, there is growing interest in Blastocystis as a potential enteric pathogen, and the possible role of domestic and in-contact animals as reservoirs for human infection. Domestic animals in shelter environments are commonly recognized to be at higher risk for carriage of enteropathogens. The purpose of this study was to determine the frequency of infection of shelt...

  3. The Effect of Hominis Placenta Herbal Acupuncture on Bell's palsy

    Directory of Open Access Journals (Sweden)

    Yun Jeong-hun

    2000-07-01

    Full Text Available This report was done to observe the effect of Hominis placenta herbal acupuncture on Bell's palsy. The study group comprised 16 patients who arrived at Woo-suk university oriental hospital from January, 1999 till January, 2000 for Bell's palsy. All patients were divided into two group. One was herbal acupunture group, and the other was control group. Acupunture group was done herbal acupuncture therapy on the facial acupuncture points. Followings are achievement and a term of each group. In herbal acupuncture group, 100% motor recovery was 7 case, 75% was 1 case, and 25% motor recovery term was 7.38±5.21 days, 50% was 11.00±6.16 days, 75% was 15.13±9.55 days, 100% was 23.14±7.97 days. In control group, 100% motor recovery was 4 case, 75% was 2 case, 25% below was 2 case and 25% motor recovery term was 11.17±4.96days, 50% was 18.17±6.82 days, 75% was 29.50±6.95 days, 100% was 44.00±11.49 days. The above results indicate that Hominis placenta herbal acupuncture is a useful effect on Bell's palsy. thus, continuous herbal acupunture study will be needed for more clinical application on Bell' palsy.

  4. Activity of medicinal plants from Ghana against the parasitic gut protist Blastocystis

    DEFF Research Database (Denmark)

    Bremer Christensen, Charlotte; Soelberg, Jens; Stensvold, Christen R

    2015-01-01

    ETHNOPHARMACOLOGICAL RELEVANCE: The plants tested in this study were examples of plants historically used to treat or alleviate several types of stomach disorders manifested by e.g. stomachache, diarrhoea or dysentery. These plants have been consumed typically as a decoction, sometimes mixed......; an ethanolic, a warm, and a cold water extract, at a final concentration of 1mg/mL for the initial screening, and in a range from 0.0156 to 1mg/mL for determination of inhibitory concentrations. The obligate anaerobic parasitic gut protist Blastocystis (subtype 4) was used as a 48h old subcultivated isolate...... (IC50) was derived after 24h and 48h. Antimicrobial activity was tested against two Gram-positive and two Gram-negative bacteria for all 24 plant parts at a final concentration of 1mg/mL. RESULTS: Screening of the 24 different plant parts showed significant anti-Blastocystis activity of six...

  5. Possible involvement of Mycoplasma hominis in inhibiting the formation of biofilms by uropathogenic Escherichia coli (UPEC).

    Science.gov (United States)

    Oh, Sangnam; Go, Gwang-Woong; Choi, Nag-Jin; Oh, Sejong; Kim, Younghoon

    2013-01-01

    Here we examined the involvement of Mycoplasma hominis in the formation of biofilms by uropathogenic Escherichia coli (UPEC) strain CFT073. Initially, we thought that M. hominis does not affect the fitness of UPEC, including the growth and production of signaling molecules, such as autoinducer-2 and indole. We found, however, that the presence of M. hominis significantly decreased the degree of biofilm formation by UPEC CFT073 (approximately a 60% reduction for 10(5) ccu/mL of M. hominis as compared with UPEC alone). We also found that it had a slight effect in inhibiting the attachment and cytotoxicity of UPEC CFT073. These findings are specific to these UPEC strains rather than to enterohemorrhagic E. coli (EHEC) strains, found in normal intestinal flora. In addition, we performed whole-transcriptome profiling and quantitative real-time polymerase chain reaction (qRT-PCR) analysis. This indicated that the PhoPQ system and the anti-termination protein (encoded by ybcQ) were involved in the reduction of biofilm formation by M. hominis (corroborated by qRT-PCR). Furthermore, our results indicate that M. hominis raises the degree of transcription of toxin genes, including hha and pasT. Hence, we suggest a possible role of M. hominis in affecting the formation of biofilms by UPEC in the urinary tract.

  6. El tópico optimum non nasci y las argumentaciones humanísticas de miseria hominis.

    Directory of Open Access Journals (Sweden)

    Gómez, Jesús

    2010-06-01

    Full Text Available The paradigm of eulogies about human dignity or indignity includes, with rhetorical variations, topoi authorized by the Greek and Latin tradition; amongst them, the topos of seeing life as a punishment, therefore it being better not being born (optimum non nasci or dying as soon as possible. This study examines Sedeño’s interpretation of this topos in his Coloquio de bienaventuranza (1536, according to the humanistic discussions de miseria hominis. Sedeño recreates Solon’s reasoning, which stems from his interview with king Croesus (following Herodotus account, without resorting to the mystery of salvation or any other Christian beliefs, therefore highlighting the singularity of his viewpoint in the humanistic debate about human happiness.El paradigma de las apologías sobre la dignidad o indignidad humana incluye, con variantes retóricas, tópicos autorizados por la tradición grecolatina, entre los cuales destaca el que entiende la vida como castigo, y que estima que es mejor no nacer (optimum non nasci o, en su defecto, morir lo más pronto posible. El presente trabajo examina la utilización que hace Sedeño, en su Coloquio de bienaventuranza (1536, del mencionado tópico a la luz de las argumentaciones humanísticas de miseria hominis. Sedeño recrea el razonamiento de Solón, derivado de su entrevista con el rey Creso según el relato de Heródoto, sin apelar desde una perspectiva religiosa al misterio de la salvación ni a otras creencias propias de la fe cristiana, por lo que sobresale la singularidad del punto de vista que introduce en el debate humanístico sobre la felicidad humana.

  7. Cytokine changes in colonic mucosa associated with Blastocystis spp. subtypes 1 and 3 in diarrhoea-predominant irritable bowel syndrome.

    Science.gov (United States)

    Yakoob, Javed; Abbas, Zaigham; Usman, Muhammad Waqas; Sultana, Aisha; Islam, Muhammad; Awan, Safia; Ahmad, Zubair; Hamid, Saeed; Jafri, Wasim

    2014-06-01

    We determined cytokines (e.g. interleukin-8, 10, 12 and TNF-α) expression by peripheral blood mononuclear cells (PBMCs) and in rectal mucosa in diarrhoea-predominant irritable bowel syndrome (D-IBS) with Blastocystis spp. Eighty patients with D-IBS and Blastocystis spp. infection were classified as 'cases' and 80 with D-IBS without Blastocystis spp. infection were classified as 'control'. Cases were subdivided into D-IBS and Blastocystis sp. defined type 1 (subtype-specific primer SB83) and type 3 (SB227). Stool microscopy and culture were performed. Rectal biopsies were obtained for histology and cytokines by real-time PCR for mRNA expression of cytokines. PBMCs IL-8 was similar in different groups but in type 1, IL-8mRNA was increased compared with type 3 (P = 0·001) and control (P = 0·001). In type 1, IL-10 by PBMCs had a low mean value (14·5±1·6) compared with (16·7±1·5) type 3 and (16±2·3) in controls (P<0·001 and P<0·001, respectively). In Blastocystis sp. type 1, low IL-10 was associated with lymphocyte and plasma cell infiltration (P = 0·015 and P = 0·002, respectively). In Blastocystis sp. type 1 and type 3, IL-12 was associated with goblet cell depletion 23 (85%) (P<0·001) and 8 (29%) (P = 0·037), respectively. In Blastocystis sp. type 1, low IL-10 was associated with a proinflammatory response characterized by IL-8.

  8. Molecular Phylogenies of Blastocystis Isolates from Different Hosts: Implications for Genetic Diversity, Identification of Species, and Zoonosis

    Science.gov (United States)

    Noël, Christophe; Dufernez, Fabienne; Gerbod, Delphine; Edgcomb, Virginia P.; Delgado-Viscogliosi, Pilar; Ho, Lip-Chuen; Singh, Mulkit; Wintjens, René; Sogin, Mitchell L.; Capron, Monique; Pierce, Raymond; Zenner, Lionel; Viscogliosi, Eric

    2005-01-01

    Small-subunit (SSU) rRNA gene sequences were obtained by PCR from 12 Blastocystis isolates from humans, rats, and reptiles for which elongation factor 1α (EF-1α) gene sequences are already available. These new sequences were analyzed by the Bayesian method in a broad phylogeny including, for the first time, all Blastocystis sequences available in the databases. Phylogenetic trees identified seven well-resolved groups plus several discrete lineages that could represent newly defined clades. Comparative analysis of SSU rRNA- and EF-1α-based trees obtained by maximum-likelihood methods from a restricted sampling (13 isolates) revealed overall agreement between the two phylogenies. In spite of their morphological similarity, sequence divergence among Blastocystis isolates reflected considerable genetic diversity that could be correlated with the existence of potentially ≥12 different species within the genus. Based on this analysis and previous PCR-based genotype classification data, six of these major groups might consist of Blastocystis isolates from both humans and other animal hosts, confirming the low host specificity of Blastocystis. Our results also strongly suggest the existence of numerous zoonotic isolates with frequent animal-to-human and human-to-animal transmissions and of a large potential reservoir in animals for infections in humans. PMID:15634993

  9. Strain-Dependent Induction of Human Enterocyte Apoptosis by Blastocystis Disrupts Epithelial Barrier and ZO-1 Organization in a Caspase 3- and 9-Dependent Manner

    Directory of Open Access Journals (Sweden)

    Zhaona Wu

    2014-01-01

    Full Text Available Blastocystis is an emerging protistan parasite colonizing the human intestine. It is frequently reported to cause general intestinal symptoms of vomiting, diarrhea, and abdominal pain. We recently demonstrated that Blastocystis rearranged cytoskeletal proteins and induced intestinal epithelial barrier compromise. The effect of Blastocystis on enterocyte apoptosis is unknown, and a possible link between microbially induced enterocyte apoptosis and increased epithelial permeability has yet to be determined. The aim of this study is to assess if Blastocystis induces human enterocyte apoptosis and whether this effect influences human intestinal epithelial barrier function. Monolayers of polarized human colonic epithelial cell-line Caco-2 were incubated with Blastocystis subtype 7 and subtype 4. Assays for both early and late markers of apoptosis, phosphatidylserine externalization, and nuclear fragmentation, respectively, showed that Blastocystis ST-7, but not ST-4, significantly increased apoptosis in enterocytes, suggesting that Blastocystis exhibits host specificity and strain-to-strain variation in pathogenicity. ST-7 also activated Caco-2 caspases 3 and 9 but not 8. ST-7 induced changes in epithelial resistance, permeability, and tight junction (ZO-1 localization. Pretreatment of Caco-2 monolayers with a pan-caspase inhibitor z-VAD-fmk significantly inhibited these changes. This suggests a role for enterocyte apoptosis in Blastocystis-mediated epithelial barrier compromise in the human intestine.

  10. An initial survey of the cattle grub Dermatobia hominis (L. Jr.) in Nicaragua.

    Science.gov (United States)

    Villarino, Mario A; Garcia, Omar; Fussell, Weyman; Preston, Kelly; Wagner, Gale G

    2003-12-12

    After the civil war and the Hurricane-Mitch disaster, cattlemen in Nicaragua were forced to transport their cattle from lowland areas to higher, dryer areas of the country. These areas are natural ecological niches for the cattle grub Dermatobia hominis (L. Jr.) (Diptera: Cuterebridae). To determine the importance of this infestation, the Agricultural and Livestock-Forestry Ministry selected a central area of Nicaragua to run a pioneer survey program to acquire information about hosts involved, number of cases, treatments applied and general knowledge of 42 farmers about the life cycle of the parasite. The subjects were either farm owners or farm managers. Ninety-five percentage of the farms indicated cases of D. hominis infestation in their animals, with cattle being the most affected host (100% of the affected farms). There was poor understanding of the D. hominis life cycle, vectors and control methods. A misuse of insecticides for the treatment of larval infestation by D. hominis was indicated.

  11. [Effects of the symbiosis of Trichomonas vaginalis with Mycoplasma hominis on ferredoxin gene].

    Science.gov (United States)

    Liu, Xiaodong; Wen, Wenjing; Xue, Changgui

    2011-08-01

    We isolated 30 Trichomonas vaginalis for the PCR detection from the gynecological outpatients in the Affiliated Hospital of Zhengzhou University using the specific 16s rDNA primers of Mycoplasma hominis. The results showed that there were 25 cases of Mycoplasma hominis infection, with the infection rate of 83.33%. This gave a clew that the symbiosis of Trichomonas vaginalis with Mycoplasma hominis may be of certain generality in China. We sequenced the ferredoxin gene of 10 Trichomonas vaginalis where 5 Mycoplasma hominis were positive and five negative, and found that the ferredoxin (Fd) gene of the 10 Trichomonas vaginalis were exactly the same. But compared to the genes in the GenBank, a comparative analysis of the gene revealed that there were 3 more ctg bases at the 200th position of encoding leucine, but this did not lead to changes in reading frame. The gene homology was 99%.

  12. Pentatrichomonas hominis: prevalence and molecular characterization in humans, dogs, and monkeys in Northern China.

    Science.gov (United States)

    Li, Wen-Chao; Ying, Meng; Gong, Peng-Tao; Li, Jian-Hua; Yang, Ju; Li, He; Zhang, Xi-Chen

    2016-02-01

    Pentatrichomonas hominis is an anaerobic amitochondrial flagellated protist that primarily colonizes the large intestines of a number of species, including cats, dogs, nonhuman primates, and humans. The prevalence of this parasite in dogs, monkeys, and humans is, however, poorly understood. In this study, a total of 362 fecal samples including 252 dogs, 60 monkeys, and 50 humans from northern China were collected for an epidemiological survey of P. hominis infection.The average prevalence of P. hominis infection determined by nested PCR was 27.38% (69/252), 4.00% (2/50), and 46.67% (28/60) in dogs, humans, and monkeys, respectively. The prevalence was significantly higher in 6-month-old dogs (41.53%) and children (7.69%) than in older dogs (14.39%) and adults (0%) (P monkeys, and humans, especially in children and young dogs. Given the infection prevalence, P. hominis may pose a risk of zoonotic and anthroponotic transmission.

  13. Mycoplasma hominis septic arthritis and common variable immunodeficiency in a postpartum patient:a case report

    Institute of Scientific and Technical Information of China (English)

    Randy A. McCool

    2012-01-01

    Postpartum patients with an unrecognized primary immunodeficiency disease, including common variable immunodeficiency, demonstrate increased susceptibility to Mycoplasma hominis infection. Diagnosis, treatment, and clinical course in a postpartum patient presenting with joint pain and episodic fever are presented.

  14. Cardiobacterium hominis Endocarditis: A Case Report and Review of the Literature

    Directory of Open Access Journals (Sweden)

    Andrew Walkty

    2005-01-01

    Full Text Available The present case report describes the clinical course of a patient who presented with Cardiobacterium hominis endocarditis. A review of the literature follows the case presentation. C hominis, a fastidious Gram-negative bacillus, is a member of the HACEK group of microorganisms (Haemophilus species, Actinobacillus actinomycetemcomitans, C hominis, Eikenella corrodens and Kingella kingae. Endocarditis caused by C hominis is uncommon and generally follows a subacute course. Patients may present with constitutional symptoms, symptoms related to valvular destruction or symptoms secondary to embolic events. Diagnosis requires identification of the pathogen from blood or vegetation by either culture or molecular techniques. Blood cultures may require prolonged incubation, highlighting the importance of incubating blood cultures for at least two to three weeks in patients with suspected endocarditis. In the past, C hominis was generally sensitive to penicillin. However, reports of beta-lactamase-producing C hominis have appeared in the literature over the past decade. The current recommendation for first-line treatment is a third-generation cephalosporin (ceftriaxone for four weeks (six weeks if a prosthetic valve is in place.

  15. Ureaplasma urealyticum and Mycoplasma hominis sensitivity to bacteriocins produced by two Lactobacilli strains.

    Science.gov (United States)

    Daniele, M; Ruiz, F; Pascual, L; Barberis, L

    2011-10-01

    The purpose of the present study was to determine the inhibitory activities of two bacteriocins, produced by lactobacilli, against genital mycoplasmas. In this study, infections produced by genital mycoplasmas were studied; of these, 1.3% were caused by Mycoplasma hominis, 10.7% by Ureaplasma urealyticum and 5.6% by U. urealyticum + M. hominis. U. urealyticum was isolated from 75 out of 123 patients with genital mycoplasmas, while M. hominis was isolated from 9 patients (7.3%) and both U. urealyticum and M. hominis from 39 patients (31.7%). Bacteriocins, L23 and L60, produced by Lactobacillus fermentum and L. rhamnosus, respectively, appear to be two novel inhibitors of bacterial infection with potential antibacterial activity. Both bacteriocins proved to be active against 100% of strains tested; MICs of bacteriocin L23 ranged between 320 and 160 UA ml(-1) for 78% of the M. hominis strains and between 320 and 80 UA ml(-1) for 95% of the U. urealyticum strains. In addition, bacteriocin L60 was still active at 160 UA ml(-1) for a high percentage (56%) of M. hominis strains, and at 80 UA ml(-1) for 53% of the U. urealyticum strains. Interestingly, these antimicrobial substances produced by lactobacilli showed an inhibitory activity against genital mycoplasmas even when diluted. Altogether, our study indicates that the bacteriocins, L23 and L60, are good candidates for the treatment or prevention of genital infections in women.

  16. Clarifying the Cryptic Host Specificity of Blastocystis spp. Isolates from Alouatta palliata and A. pigra Howler Monkeys

    Science.gov (United States)

    Villanueva-Garcia, Claudia; Gordillo-Chavez, Elias Jose; Lopez-Escamilla, Eduardo; Rendon-Franco, Emilio; Muñoz-Garcia, Claudia Irais; Gama, Lilia; Martinez-Flores, Williams Arony; Gonzalez-Rodriguez, Nayeli; Romero-Valdovinos, Mirza; Diaz-Lopez, Hilda; Galian, Jose; Villalobos, Guiehdani; Maravilla, Pablo; Martinez-Hernandez, Fernando

    2017-01-01

    Although the presence of cryptic host specificity has been documented in Blastocystis, differences in infection rates and high genetic polymorphism within and between populations of some subtypes (ST) have impeded the clarification of the generalist or specialist specificity of this parasite. We assessed the genetic variability and host specificity of Blastocystis spp. in wild howler monkeys from two rainforest areas in the southeastern region of Mexico. Fecal samples of 225 Alouatta palliata (59) and A. pigra (166) monkeys, belonging to 16 sylvatic sites, were analyzed for infection with Blastocystis ST using a region of the small subunit rDNA (SSUrDNA) gene as a marker. Phylogenetic and genetic diversity analyses were performed according to the geographic areas where the monkeys were found. Blastocystis ST2 was the most abundant (91.9%), followed by ST1 and ST8 with 4.6% and 3.5%, respectively; no association between Blastocystis ST and Alouatta species was observed. SSUrDNA sequences in GenBank from human and non-human primates (NHP) were used as ST references and included in population analyses. The haplotype network trees exhibited different distributions: ST1 showed a generalist profile since several haplotypes from different animals were homogeneously distributed with few mutational changes. For ST2, a major dispersion center grouped the Mexican samples, and high mutational differences were observed between NHP. Furthermore, nucleotide and haplotype diversity values, as well as migration and genetic differentiation indexes, showed contrasting values for ST1 and ST2. These data suggest that ST1 populations are only minimally differentiated, while ST2 populations in humans are highly differentiated from those of NHP. The host generalist and specialist specificities exhibited by ST1 and ST2 Blastocystis populations indicate distinct adaptation processes. Because ST1 exhibits a generalist profile, this haplotype can be considered a metapopulation; in contrast

  17. Molecular Epidemiology of Blastocystis sp. in Various Animal Groups from Two French Zoos and Evaluation of Potential Zoonotic Risk

    Science.gov (United States)

    Moriniere, Romain; Gantois, Nausicaa; Benamrouz-Vanneste, Sadia; Delgado-Viscogliosi, Pilar; Guyot, Karine; Li, Luen-Luen; Monchy, Sébastien; Noël, Christophe; Poirier, Philippe; Nourrisson, Céline; Wawrzyniak, Ivan; Delbac, Frédéric; Bosc, Stéphanie; Chabé, Magali; Petit, Thierry; Certad, Gabriela; Viscogliosi, Eric

    2017-01-01

    Blastocystis sp. is a common intestinal parasite infecting humans and a wide range of animals worldwide. It exhibits an extensive genetic diversity and 17 subtypes (STs) have thus far been identified in mammalian and avian hosts. Since several STs are common to humans and animals, it was proposed that a proportion of human infections may result from zoonotic transmission. However, the contribution of each animal source to human infection remains to be clarified. Therefore, the aim of this study was to expand our knowledge of the epidemiology and host specificity of this parasite by performing the largest epidemiological survey ever conducted in animal groups in terms of numbers of species screened. A total of 307 stool samples from 161 mammalian and non-mammalian species in two French zoos were screened by real-time PCR for the presence of Blastocystis sp. Overall, 32.2% of the animal samples and 37.9% of the species tested were shown to be infected with the parasite. A total of 111 animal Blastocystis sp. isolates were subtyped, and 11 of the 17 mammalian and avian STs as well as additional STs previously identified in reptiles and insects were found with a varying prevalence according to animal groups. These data were combined with those obtained from previous surveys to evaluate the potential risk of zoonotic transmission of Blastocystis sp. through the comparison of ST distribution between human and animal hosts. This suggests that non-human primates, artiodactyls and birds may serve as reservoirs for human infection, especially in animal handlers. In contrast, other mammals such as carnivores, and non-mammalian groups including reptiles and insects, do not seem to represent significant sources of Blastocystis sp. infection in humans. In further studies, more intensive sampling and screening of potential new animal hosts will reinforce these statements and expand our understanding of the circulation of Blastocystis sp. in animal and human populations. PMID

  18. Molecular mechanism of fluoroquinolones resistance in Mycoplasma hominis clinical isolates.

    Science.gov (United States)

    Meng, Dong-Ya; Sun, Chang-Jian; Yu, Jing-Bo; Ma, Jun; Xue, Wen-Cheng

    2014-01-01

    To evaluate the molecular mechanism of fluoroquinolones resistance in Mycoplasma hominis (MH) clinical strains isolated from urogenital specimens. 15 MH clinical isolates with different phenotypes of resistance to fluoroquinolones antibiotics were screened for mutations in the quinolone resistance-determining regions (QRDRs) of DNA gyrase (gyrA and gyrB) and topoisomerase IV (parC and parE) in comparison with the reference strain PG21, which is susceptible to fluoroquinolones antibiotics. 15 MH isolates with three kinds of quinolone resistance phenotypes were obtained. Thirteen out of these quinolone-resistant isolates were found to carry nucleotide substitutions in either gyrA or parC. There were no alterations in gyrB and no mutations were found in the isolates with a phenotype of resistance to Ofloxacin (OFX), intermediate resistant to Levofloxacin (LVX) and Sparfloxacin (SFX), and those susceptible to all three tested antibiotics. The molecular mechanism of fluoroquinolone resistance in clinical isolates of MH was reported in this study. The single amino acid mutation in ParC of MH may relate to the resistance to OFX and LVX and the high-level resistance to fluoroquinolones for MH is likely associated with mutations in both DNA gyrase and the ParC subunit of topoisomerase IV.

  19. Molecular mechanism of fluoroquinolones resistance in Mycoplasma hominis clinical isolates

    Directory of Open Access Journals (Sweden)

    Meng Dong-Ya

    2014-01-01

    Full Text Available To evaluate the molecular mechanism of fluoroquinolones resistance in Mycoplasma hominis (MH clinical strains isolated from urogenital specimens. 15 MH clinical isolates with different phenotypes of resistance to fluoroquinolones antibiotics were screened for mutations in the quinolone resistance-determining regions (QRDRs of DNA gyrase (gyrA and gyrB and topoisomerase IV (parC and parE in comparison with the reference strain PG21, which is susceptible to fluoroquinolones antibiotics. 15 MH isolates with three kinds of quinolone resistance phenotypes were obtained. Thirteen out of these quinolone-resistant isolates were found to carry nucleotide substitutions in either gyrA or parC. There were no alterations in gyrB and no mutations were found in the isolates with a phenotype of resistance to Ofloxacin (OFX, intermediate resistant to Levofloxacin (LVX and Sparfloxacin (SFX, and those susceptible to all three tested antibiotics. The molecular mechanism of fluoroquinolone resistance in clinical isolates of MH was reported in this study. The single amino acid mutation in ParC of MH may relate to the resistance to OFX and LVX and the high-level resistance to fluoroquinolones for MH is likely associated with mutations in both DNA gyrase and the ParC subunit of topoisomerase IV.

  20. Myiasis caused by Dermatobia hominis: countries with increased risk for travelers going to neotropic areas.

    Science.gov (United States)

    Villalobos, Guiehdani; Vega-Memije, Maria Elisa; Maravilla, Pablo; Martinez-Hernandez, Fernando

    2016-10-01

    Here, we review the human botfly (Dermatobia hominis), which belongs to a group of Diptera generically known as "myiasis-causing flies," characterized by the ability of their larvae to develop in animal flesh. In addition to its medical and economic importance, there is an academic interest in this botfly because of its peculiar biology, particularly because a phoretic diptera is needed to complete the life cycle. The larvae penetrate the host's skin, causing furuncle-like lesions that are pruritic, painful, and resemble subcutaneous nodules, producing irreversible perforations in the skin. Although D. hominis is distributed from Mexico to Argentina, a review performed by our working group from 1999 to 2015 determined that the countries with the highest infection rates in travelers are Belize, Bolivia, and Brazil. Interestingly, infected men show a higher variation in the distribution of the lesions than in women. Many treatment schemes have been suggested, including the application of highly dense liquids to the lesion to cause anoxia in the D. hominis larvae. We showed, for the first time, a Bayesian inference between D. hominis and other myiasis-causing flies. The flies grouped into two main clusters according to their capacity to produce facultative and obligatory myiasis, and D. hominis was phylogenetically close to Cuterebra spp.

  1. Detection of multiple zoonotic infectious agents in cattle with Blastocystis, Cryptosporidium, Giardia, and Enterocytozoon by molecular and microscopic methods

    Science.gov (United States)

    Feces from 47 dairy cattle ranging in age from neonates to multiparous cows were examined and 9, 10, 24, and 17 were found positive for Blastocystis spp., Cryptosporidium spp., Giardia duodenalis, and Enterocytozoon bieneusi, respectively, by PCR. Eight 3- to 5-month-old cattle were concurrently i...

  2. Detection of concurrent infection of dairy cattle with Blastocystis, Cryptosporidium, Giardia, and Enterocytozoon by molecular and microscopic methods

    Science.gov (United States)

    Of fecal specimens examined from 47 dairy cattle ranging in age from neonates to multiparous cows, 9, 10, 24, and 17 were positive for Blastocystis spp., Cryptosporidium spp., Giardia duodenalis, and Enterocytozoon bieneusi, respectively, as determined by PCR. Eight 3- to 5-month-old cattle were co...

  3. Draft genome sequence of Staphylococcus hominis strain Hudgins isolated from human skin implicates metabolic versatility and several virulence determinants

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    Shelby Calkins

    2016-12-01

    Full Text Available Staphylococcus hominis is a predominant member of the human skin microbiome. We here report on the genomic analysis of Staphylococcus hominis strain Hudgins that was isolated from the wrist area of human skin. The partial genome assembly of S. hominis Hudgins consists of 2,211,863 bp of DNA with 2174 protein-coding genes and 90 RNA genes. Based on the genomic analysis of KEGG pathways, the organism is expected to be a versatile heterotroph potentially capable of hydrolyzing the sugars glucose, fructose, mannose, and the amino acids alanine, aspartate, glutamate, glycine, threonine, cysteine, methionine, valine, isoleucine, leucine, lysine, arginine, phenylalanine, tyrosine, and tryptophan for energy production through aerobic respiration, with occasional lactate and acetate fermentation. Evidence for poly-gamma glutamate capsule and type IV Com system pili were identified in the genome. Based on COG analysis, the genome of S. hominis Hudgins clusters away from the previously published S. hominis genome ZBW5.

  4. Comparison of commercially available media for detection and isolation of Ureaplasma urealyticum and Mycoplasma hominis.

    OpenAIRE

    Broitman, N L; Floyd, C M; Johnson, C. A.; de la Maza, L M; Peterson, E M

    1992-01-01

    The Mycotrim Triphasic flask system (Irvine Scientific, Irvine, Calif.) was compared with a system composed of Mycotrim GU broth (Irvine Scientific) and A7 or A8 agar (Remel, Lenexa, Kans.) for the ability to detect Ureaplasma urealyticum and Mycoplasma hominis from 129 genital specimens. Of the 64 specimens positive for U. urealyticum, 25, 98, and 100% were detected on Mycotrim Triphasic agar and A7 and A8 agars, respectively. All 18 specimens that grew M. hominis were detected by A7 and A8 ...

  5. Prevalence of Mycoplasma genitalium, Mycoplasma hominis and Chlamydia trachomatis among Danish patients requesting abortion

    DEFF Research Database (Denmark)

    Baczynska, Agata; Hvid, Malene; Lamy, P;

    2008-01-01

    The aim of the study was to determine lower genital tract carriage rate of Mycoplasma genitalium (M. genitalium) and to compare it to the carriage rates of Mycoplasma hominis (M. hominis ) and Chlamydia trachomatis (C. trachomatis) among 102 women requesting termination of pregnancy at the Horsens...... Hospital in Denmark. Real-Time PCR was used for the detection of bacterial DNA, and the presence of antibodies to the three microorganisms was determined by ELISA and immunoblotting. Real-Time PCR detected M. genitalium in one swab sample (0.98%) only, while the prevalence of C. trachomatis was high (15...

  6. Late prosthetic valve endocarditis due to Cardiobacterium hominis, an unusual complication

    Directory of Open Access Journals (Sweden)

    Shivaprakasha S

    2007-01-01

    Full Text Available We report a case of prosthetic valve endocarditis caused by Cardiobacterium hominis in a patient who had undergone atrial septal defect closure and mitral valve replacement of the heart in 1978. He presented with pyrexia of unknown origin and congestive cardiac failure. Investigations revealed infective endocarditis of prosthetic valve in mitral portion. Blood culture samples grew C. hominis . The patient was empirically started on vancomycin and gentamicin intravenously and ceftriaxone was added after isolation of the organism. Though subsequent blood cultures were negative, patient remained in congestive cardiac failure and died due to complications.

  7. Comparison of methods for detection of Blastocystis infection in routinely submitted stool samples, and also in IBS/IBD Patients in Ankara, Turkey.

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    Funda Dogruman-Al

    Full Text Available BACKGROUND: This study compared diagnostic methods for identifying Blastocystis in stool samples, and evaluated the frequency of detection of Blastocystis in patients with irritable bowel syndrome (IBS and inflammatory bowel disease (IBD. RESULTS AND DISCUSSION: From a set of 105 stool specimens submitted for routine parasitological analysis, 30 were identified as positive for Blastocystis by the culture method. From that group of 30 positives, Lugol's stain, trichrome staining, and an immunofluorescence assay identified 11, 15, and 26 samples as positive respectively. Using culture as a standard, the sensitivity of Lugol's stain was 36.7%, trichrome staining was 50%, and the IFA stain was 86.7%. The specificity of Lugol's stain was 91%, trichrome staining was 100%, and the IFA stain was 97.3%. In the group of 27 IBS and IBD patients, using all methods combined, we detected Blastocystis in 67% (18/27 of the patients. Blastocystis was detected in 33% (2/6 of IBD patients and 76% (16/21 of IBS patients. For comparison, trichrome staining alone, the method most frequently used in many countries, would have only identified Blastocystis infection in 29% (6/21 of the IBS patients. No parasitic co-infections were identified in the IBS/IBD patients. Most Blastocystis-positive IBS/IBD patients were over 36 with an average length of illness of 4.9 years. CONCLUSIONS: Most IBS patients in this study were infected with Blastocystis. IFA staining may be a useful alternative to stool culture, especially if stool specimens have been chemically preserved.

  8. Subtype distribution of Blastocystis isolates from synanthropic and zoo animals and identification of a new subtype

    DEFF Research Database (Denmark)

    Stensvold, C. R.; Alfellani, M. A.; Nørskov-Lauritsen, S.;

    2009-01-01

    Blastocystis isolates from 56 Danish synanthropic and zoo animals, 62 primates primarily from United Kingdom (UK) collections and 16 UK primate handlers were subtyped by PCR, sequencing and phylogenetic analysis. A new subtype (ST) from primates and artiodactyls was identified and designated...... infections from primates by their handlers had occurred in these cases. Data from published studies of non-human primates, other mammals and birds were collected and interpreted to generate a comprehensive overview on the ST distribution in such animals. On the basis of information on 438 samples......, it was found that Blostocystis from primates belong mainly to ST1, ST2, ST3, ST5 and ST8, ungulates and dogs mainly ST1, ST2, ST3, ST5 and ST10, rodents ST4 and birds mainly ST6 and ST7. The data indicate moderate host specificity, most clearly exemplified by the fact that STs isolated from avian and non...

  9. A case of cutaneous myiasis due to Dermatobia hominis in Japan.

    Science.gov (United States)

    Nagamori, Katsushi; Katayama, Toshiko; Kumagai, Masahiro

    2007-08-01

    We report the 34th imported case of cutaneous myiasis caused by Dermatobia hominis in Japan, which is not a habitat of the fly. A 41-year-old Japanese man noticed an insect-sting-like papule on his left upper back during his stay in Ecuador in March 2004. After his return home, the lesion gradually increased to become a red subcutaneous nodule with a central pore from which serosanguineous fluid drained. Because antimicrobial treatment under a diagnosis of inflammatory atheroma was ineffective, the lesion was incised and a 3rd instar larva of D. hominis was then found and removed. We checked the literature on D. hominis myiasis reported from Japan, and noted the fact, which nobody had previously pointed out, that in Japan only one case of D. hominis myiasis had been diagnosed correctly before a larva was found, and most of the cases were misdiagnosed and inappropriately treated, including 11 cases given unnecessary resection of the nodules. Doctors in Japan should be aware of myiasis so that patients are neither anxious about the disease nor suffer pain, and doctors avoid performing unnecessary resections of the lesions.

  10. Charcterization of Type Ⅱ Topoisomerase Gene Mutations in Clinical Isolates of Mycoplasma Hominis Resistant to Fluoroquinolones

    Institute of Scientific and Technical Information of China (English)

    吴移谋; 张文波; 姚艳冰

    2002-01-01

    Objective: To analyze type Ⅱ topoisomerase genes inclinical isolates of fluoroquinolone-resistant Mycoplasmahominis. Methods: Eight isolates of M.hominis cross resistant to 6fluoroquinolones were selected from 103 clinical strains ofM.hominis using a broth microdilution method. Type IItopoisomerase genes were amplified by PCR and directlysequenced. Nucleotide sequences were compared to sequencesfrom a susceptible strain (M.hominis PG2I). Results: MICs of resistant Mh isolates were 4- to 512-fold higher than MICs from the susceptible reference strain.Sequence comparison revealed a C to T change at 113nt ingyrA QRDR led to the substitution of Ser83 by Leucine and noamino acid change in gyrB. A change of G to T at 134nt inparC QRDR led to the substitution of Ser80 by Isoleucine anda G to A change at 70nt in parE QRDR led to the substitutionof Aspartic acid by Asparagine. Conclusion: These results suggest that a C to T change atll3nt in gyrA, a G to T change at 134nt in parC and a G to Achange at 70nt in patrE are associated with fluoroquinoloneresistance of M.hominis.

  11. Dermatobia hominis in the accident and emergency department: "I've got you under my skin".

    OpenAIRE

    MacNamara, A; Durham, S

    1997-01-01

    An unusual form of larval infestation from South America is presented which, in view of increasing tourism to South america's tropical areas, may present to any accident and emergency department. Infestation with Dermatobia hominis is reviewed in terms of clinical recognition and life cycle. Techniques of removal are described.

  12. Dermatobia hominis in the accident and emergency department: "I've got you under my skin".

    Science.gov (United States)

    MacNamara, A; Durham, S

    1997-05-01

    An unusual form of larval infestation from South America is presented which, in view of increasing tourism to South america's tropical areas, may present to any accident and emergency department. Infestation with Dermatobia hominis is reviewed in terms of clinical recognition and life cycle. Techniques of removal are described.

  13. Heterogeneity of Mycoplasma hominis as detected by a probe for atp genes

    DEFF Research Database (Denmark)

    Christiansen, C; Christiansen, Gunna; Rasmussen, OF

    1987-01-01

    Use of a plasmid containing part of the atp operon of Mycoplasma PG50 as a probe in Southern blots show that this region can be used to detect the presence of Mycoplasma species in general. DNA from 14 different strains of M. hominis was analyzed for restriction fragment length polymorphism (RFLP...

  14. Development of Cultivation Technology for the Escherichia coli Recombinant Strain Producing Argininedeiminase of Mycoplasma hominis

    OpenAIRE

    Fayura, L R; Boretsky, Yu.R.; Pynyaha, Yu.V.; Martynyuk, N.B.; Skorohod, V.V.; Sybyrny, А.А.

    2014-01-01

    The recombinant Escherichia coli strain producing arginine deiminase of Mycoplasma hominis has been constructed. Storage conditions that provide stabilization of most productive clones of the producer were found. Terms for cultivation of the arginine deiminase producer using bioreactors of different volume were optimized. Highly purified samples of arginine deiminase were obtained and their longterm storage conditions were selected.

  15. Development of Cultivation Technology for the Escherichia coli Recombinant Strain Producing Argininedeiminase of Mycoplasma hominis

    Directory of Open Access Journals (Sweden)

    Fayura, L.R.

    2014-07-01

    Full Text Available The recombinant Escherichia coli strain producing arginine deiminase of Mycoplasma hominis has been constructed. Storage conditions that provide stabilization of most productive clones of the producer were found. Terms for cultivation of the arginine deiminase producer using bioreactors of different volume were optimized. Highly purified samples of arginine deiminase were obtained and their longterm storage conditions were selected.

  16. The Effects of the Hominis Placenta Herbal acupuncture on Sleep pattern disturbance

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    Youn Hyoun-min

    2005-02-01

    Full Text Available Objective : This study has been designed and performed to identify the effects of Hominis Placenta herbal acupuncture which is usually used in reducing sleep pattern disturbances. Methods : The study subjects studied included 48 patients who were admitted in hospital located in Pusan, and they were classified into 2 groups : 25 patients in the experimental group who injected Hominis Placenta herbal acupuncture and 23 patients in the control group who were treated by acupuncture. The both group injected on GB20, GB12 and HT7 for 5 days without medicine. The sleep pattern disturbance score was measured by using 15 questions according to Korean Sleep Scale A(Oh, Jin Joo. Song, Mi Soon. Kim, Shin Mi. 1998. Results & conclusions : The sleep pattern disturbance score of the experimental group who injected Hominis Placenta herbal acupuncture was significantly lower than that of the control group. (t= 7.00 p= .00 These results provided that Hominis Placenta herbal acupuncture of GB20, GB12 and HT7 was effective for relieving sleep pattern disturbances, it is need more sample's number and more treatmentt's duration.

  17. Emergence of Cryptosporidium hominis Monkey Genotype II and Novel Subtype Family Ik in the Squirrel Monkey (Saimiri sciureus) in China.

    Science.gov (United States)

    Liu, Xuehan; Xie, Na; Li, Wei; Zhou, Ziyao; Zhong, Zhijun; Shen, Liuhong; Cao, Suizhong; Yu, Xingming; Hu, Yanchuan; Chen, Weigang; Peng, Gangneng

    2015-01-01

    A single Cryptosporidium isolate from a squirrel monkey with no clinical symptoms was obtained from a zoo in Ya'an city, China, and was genotyped by PCR amplification and DNA sequencing of the small-subunit ribosomal RNA (SSU rRNA), 70-kDa heat shock protein (HSP70), Cryptosporidium oocyst wall protein, and actin genes. This multilocus genetic characterization determined that the isolate was Cryptosporidium hominis, but carried 2, 10, and 6 nucleotide differences in the SSU rRNA, HSP70, and actin loci, respectively, which is comparable to the variations at these loci between C. hominis and the previously reported monkey genotype (2, 3, and 3 nucleotide differences). Phylogenetic studies, based on neighbor-joining and maximum likelihood methods, showed that the isolate identified in the current study had a distinctly discordant taxonomic status, distinct from known C. hominis and also from the monkey genotype, with respect to the three loci. Restriction fragment length polymorphisms of the SSU rRNA gene obtained from this study were similar to those of known C. hominis but clearly differentiated from the monkey genotype. Further subtyping was performed by sequence analysis of the gene encoding the 60-kDa glycoprotein (gp60). Maximum homology of only 88.3% to C. hominis subtype IdA10G4 was observed for the current isolate, and phylogenetic analysis demonstrated that this particular isolate belonged to a novel C. hominis subtype family, IkA7G4. This study is the first to report C. hominis infection in the squirrel monkey and, based on the observed genetic characteristics, confirms a new C. hominis genotype, monkey genotype II. Thus, these results provide novel insights into genotypic variation in C. hominis.

  18. Ureaplasma Urealyticum or Mycoplasma Hominis Infections and Semen Quality of Infertile Men in Abidjan

    Institute of Scientific and Technical Information of China (English)

    Zinzendorf NY; Kouassi-Agbessi BT; Lathro JS; Don C; Kouadio L; Loukou YG

    2008-01-01

    Objective To determine the prevalence of U.urealvticum and M.hominis in semen samples collected from men admitted in clinic for infertility,and to compare the quality of these semen samples.Methods A total of 1058 semen samples collected were investigated.Sperm semiological assays were performed according to the guidelines of the World Health Organisation(WHO).Semen were examined by Mycoplasma IST for the detection of mycoplasma.Semen culture on agar media was used to detect other microorganisms.Chlamydia was detected using direct fluorescent assay(DFA)of Clamydia Trachomatis.Results Among 1058 semen samples,microorganisms were detected in 638(60.3%).The infected sperms consisted of mycoplasma alone in 507 cases(47.9%).mycoplasma and other microorganisms in 98(9.3%),giving in all 605(57.2%)samples infected with mycoplasma.The last 33(3.1%)consisted of other microorganisms alone.The frequency of U.urealyticum.M.hominis and mixed genital infections detected in semen samples of infertile men were 39%,23.8% and 5.6%,respectively.The rates of abnormal semen parameters recorded among patients infected with mycoplasma were for volume(22.2%-25%),viscosity(29.6%-43.5%),pH(64.7%-72.9%),motility(80.8%-93.8%),morphology(36.3%-47.9%),sperm concentration(53.3%-58.3%)and leukocyte count(51.4%-58.3%).Conclusion Frequency of U.urealyticum infection was higher than that of M.hominis.Mycoplasma infections were associated with disorders of pH,motility and sperm concentration.In addition M.hominis infection affected spermatozoa morphology.Therefore,screening of U.urealyticum and M.hominis for routine semen analysis is clinically relevant in Abidjan.

  19. Co-infection by Tritrichomonas foetus and Pentatrichomonas hominis in asymptomatic cats

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    Caroline Spitz dos Santos

    2015-12-01

    Full Text Available Abstract: Tritrichomonas foetus, a parasite well known for its significance as a venereally transmitted pathogen in cattle, has been identified as a cause of chronic large bowel diarrhea in domestic cats in many countries of the world. In Brazil, several studies on the diagnosis of bovine trichomoniasis have been performed, but until now, no study was made regarding feline trichomoniasis. Thus, this is the first study to report the occurrence of T. foetus and Pentatrichomonas hominis in cats using morphological and molecular analysis. Feces from 77 cats were examined, four of which (5.2% were positive for the presence of parabasalids. Morphological analysis of stained smears revealed piriform trophozoites showing the three anterior flagella, elongated nucleus and axostyle ending abruptly in fillet, characteristic of T. foetus. In scanning and transmission electron microscopy, identification characters similar to those previously reported for T. foetus were observed. The cultures containing trophozoites were submitted for molecular analysis, which resulted positive for T. foetus DNA using specific primers (TFR3 and TFR4, and all samples were positive and subjected to sequencing in which they showed 99.7-100% similarity with another isolate sequencing of T. foetus (JX960422. Although no trophozoite with consistent morphology of P. hominis has been visualized in the samples, differential diagnosis was performed using specific primers for P. hominis (TH3 and TH5 amplicon. In three of the four samples (3.89% sequencing revealed 100% similarity when compared with another sequence of P. hominis deposited in Genbank (KC623939. Therefore, the present study revealed through the diagnostic techniques employed the simultaneous infection by T. foetus and P. hominis in the feces of cats. However, it was necessary to use more than one technique for the diagnosis of the co-infection. These results demonstrate the importance of a correct diagnosis to allow an

  20. Occurrence of Blastocystis sp. in water catchments at Malay villages and Aboriginal settlement during wet and dry seasons in Peninsular Malaysia

    Science.gov (United States)

    Noradilah, Samseh Abdullah; Lee, Ii Li; Anuar, Tengku Shahrul; Salleh, Fatmah Md; Abdul Manap, Siti Nor Azreen; Mohd Mohtar, Noor Shazleen Husnie; Azrul, Syed Muhamad; Abdullah, Wan Omar

    2016-01-01

    In the tropics, there are too few studies on isolation of Blastocystis sp. subtypes from water sources; in addition, there is also an absence of reported studies on the occurrence of Blastocystis sp. subtypes in water during different seasons. Therefore, this study was aimed to determine the occurrence of Blastocystis sp. subtypes in river water and other water sources that drained aboriginal vicinity of highly endemic intestinal parasitic infections during wet and dry seasons. Water samples were collected from six sampling points of Sungai Krau (K1–K6) and a point at Sungai Lompat (K7) and other water sources around the aboriginal villages. The water samples were collected during both seasons, wet and dry seasons. Filtration of the water samples were carried out using a flatbed membrane filtration system. The extracted DNA from concentrated water sediment was subjected to single round polymerase chain reaction and positive PCR products were subjected to sequencing. All samples were also subjected to filtration and cultured on membrane lactose glucuronide agar for the detection of faecal coliforms. During wet season, Blastocystis sp. ST1, ST2 and ST3 were detected in river water samples. Blastocystis sp. ST3 occurrence was sustained in the river water samples during dry season. However Blastocystis sp. ST1 and ST2 were absent during dry season. Water samples collected from various water sources showed contaminations of Blastocystis sp. ST1, ST2, ST3 and ST4, during wet season and Blastocystis sp. ST1, ST3, ST8 and ST10 during dry season. Water collected from all river sampling points during both seasons showed growth of Escherichia coli and Enterobacter aerogenes, indicating faecal contamination. In this study, Blastocystis sp. ST3 is suggested as the most robust and resistant subtype able to survive in any adverse environmental condition. Restriction and control of human and animal faecal contaminations to the river and other water sources shall prevent the

  1. Occurrence of Blastocystis sp. in water catchments at Malay villages and Aboriginal settlement during wet and dry seasons in Peninsular Malaysia

    Directory of Open Access Journals (Sweden)

    Samseh Abdullah Noradilah

    2016-10-01

    Full Text Available In the tropics, there are too few studies on isolation of Blastocystis sp. subtypes from water sources; in addition, there is also an absence of reported studies on the occurrence of Blastocystis sp. subtypes in water during different seasons. Therefore, this study was aimed to determine the occurrence of Blastocystis sp. subtypes in river water and other water sources that drained aboriginal vicinity of highly endemic intestinal parasitic infections during wet and dry seasons. Water samples were collected from six sampling points of Sungai Krau (K1–K6 and a point at Sungai Lompat (K7 and other water sources around the aboriginal villages. The water samples were collected during both seasons, wet and dry seasons. Filtration of the water samples were carried out using a flatbed membrane filtration system. The extracted DNA from concentrated water sediment was subjected to single round polymerase chain reaction and positive PCR products were subjected to sequencing. All samples were also subjected to filtration and cultured on membrane lactose glucuronide agar for the detection of faecal coliforms. During wet season, Blastocystis sp. ST1, ST2 and ST3 were detected in river water samples. Blastocystis sp. ST3 occurrence was sustained in the river water samples during dry season. However Blastocystis sp. ST1 and ST2 were absent during dry season. Water samples collected from various water sources showed contaminations of Blastocystis sp. ST1, ST2, ST3 and ST4, during wet season and Blastocystis sp. ST1, ST3, ST8 and ST10 during dry season. Water collected from all river sampling points during both seasons showed growth of Escherichia coli and Enterobacter aerogenes, indicating faecal contamination. In this study, Blastocystis sp. ST3 is suggested as the most robust and resistant subtype able to survive in any adverse environmental condition. Restriction and control of human and animal faecal contaminations to the river and other water sources

  2. Subtype analysis of Blastocystis sp. isolates from human and canine hosts in an urban community in the Philippines.

    Science.gov (United States)

    Belleza, Maria Luz B; Reyes, John Carlo B; Tongol-Rivera, Pilarita N; Rivera, Windell L

    2016-06-01

    Blastocystis sp. is a common gut-dwelling protist of both humans and animals. A cross-sectional survey among humans and their dogs was conducted to determine the prevalence of Blastocystis infection and to characterize the subtype (ST) distribution in an urban community in the Philippines. Fecal specimens from 1,271 humans and 145 dogs were collected and inoculated in diphasic culture medium. Prevalence of Blastocystis by culture was 13.0% (95% CI = 11.2-15.0) and 14.5% (95% CI = 9.6-21.2) for humans and dogs, respectively. A total of 168 culture isolates were genotyped using polymerase chain reaction (PCR) with seven pairs of ST-specific sequence-tagged-site (STS) primers. In humans, the ST present in this study were ST1 with 22.6% (95% CI = 17.2-29.0), ST2 with 3.1% (95% CI = 1.3-6.7), ST3 with 41.4% (95% CI= 34.9-48.6), ST4 with 14.8% (95% CI = 10.5-20.6), ST5 with 4.1% (95% CI = 2.0-8.0), and unknown ST with 13.9% (95% CI = 9.6-19.4). In dogs, the ST present in this study were ST1 with 4.3% (95% CI = 0.0-29.0), ST2 with 8.7% (95% CI = 1.3-28.0), ST3 with 17.4% (95% CI = 6.4-37.7), ST4 with 13.0% (95% CI = 3.7-33.0), ST5 with 13.0% (95% CI = 3.7-33.0), and unknown ST with 47.8% (95% CI = 29.2-67.0). This is the first study that reported Blastocystis ST4 in human and canine hosts in the Philippines.

  3. Complete Genome Sequence of Mycoplasma hominis Strain Sprott (ATCC 33131), Isolated from a Patient with Nongonococcal Urethritis

    OpenAIRE

    2015-01-01

    Presented here is the complete and annotated genome sequence of Mycoplasma hominis Sprott (ATCC 33131). The chromosome comprises 695,214 bp, which is approximately 30 kb larger than the syntenic genome of M. hominis PG21T. Tetracycline resistance of strain Sprott is most probably conferred by the tetM determinant, harbored on a mosaic transposon-like structure.

  4. Complete Genome Sequence of Mycoplasma hominis Strain Sprott (ATCC 33131), Isolated from a Patient with Nongonococcal Urethritis.

    Science.gov (United States)

    Calcutt, Michael J; Foecking, Mark F

    2015-07-09

    Presented here is the complete and annotated genome sequence of Mycoplasma hominis Sprott (ATCC 33131). The chromosome comprises 695,214 bp, which is approximately 30 kb larger than the syntenic genome of M. hominis PG21(T). Tetracycline resistance of strain Sprott is most probably conferred by the tetM determinant, harbored on a mosaic transposon-like structure.

  5. Symbiotic Association with Mycoplasma hominis Can Influence Growth Rate, ATP Production, Cytolysis and Inflammatory Response of Trichomonas vaginalis.

    Science.gov (United States)

    Margarita, Valentina; Rappelli, Paola; Dessì, Daniele; Pintus, Gianfranco; Hirt, Robert P; Fiori, Pier L

    2016-01-01

    The symbiosis between the parasitic protist Trichomonas vaginalis and the opportunistic bacterium Mycoplasma hominis is the only one currently described involving two obligate human mucosal symbionts with pathogenic capabilities that can cause independent diseases in the same anatomical site: the lower urogenital tract. Although several aspects of this intriguing microbial partnership have been investigated, many questions on the influence of this symbiosis on the parasite pathobiology still remain unanswered. Here, we examined with in vitro cultures how M. hominis could influence the pathobiology of T. vaginalis by investigating the influence of M. hominis on parasite replication rate, haemolytic activity and ATP production. By comparing isogenic mycoplasma-free T. vaginalis and parasites stably associated with M. hominis we could demonstrate that the latter show a higher replication rate, increased haemolytic activity and are able to produce larger amounts of ATP. In addition, we demonstrated in a T. vaginalis-macrophage co-culture system that M. hominis could modulate an aspect of the innate immuno-response to T. vaginalis infections by influencing the production of nitric oxide (NO) by human macrophages, with the parasite-bacteria symbiosis outcompeting the human cells for the key substrate arginine. These results support a model in which the symbiosis between T. vaginalis and M. hominis influences host-microbes interactions to the benefit of both microbial partners during infections and to the detriment of their host.

  6. Computational prediction of Mycoplasma hominis proteins targeting in nucleus of host cell and their implication in prostate cancer etiology.

    Science.gov (United States)

    Khan, Shahanavaj; Zakariah, Mohammed; Palaniappan, Sellappan

    2016-08-01

    Cancer has long been assumed to be a genetic disease. However, recent evidence supports the enigmatic connection of bacterial infection with the growth and development of various types of cancers. The cause and mechanism of the growth and development of prostate cancer due to Mycoplasma hominis remain unclear. Prostate cancer cells are infected and colonized by enteroinvasive M. hominis, which controls several factors that can affect prostate cancer growth in susceptible persons. We investigated M. hominis proteins targeting the nucleus of host cells and their implications in prostate cancer etiology. Many vital processes are controlled in the nucleus, where the proteins targeting M. hominis may have various potential implications. A total of 29/563 M. hominis proteins were predicted to target the nucleus of host cells. These include numerous proteins with the capability to alter normal growth activities. In conclusion, our results emphasize that various proteins of M. hominis targeted the nucleus of host cells and were involved in prostate cancer etiology through different mechanisms and strategies.

  7. Symbiotic Association with Mycoplasma hominis Can Influence Growth Rate, ATP Production, Cytolysis and Inflammatory Response of Trichomonas vaginalis

    Science.gov (United States)

    Margarita, Valentina; Rappelli, Paola; Dessì, Daniele; Pintus, Gianfranco; Hirt, Robert P.; Fiori, Pier L.

    2016-01-01

    The symbiosis between the parasitic protist Trichomonas vaginalis and the opportunistic bacterium Mycoplasma hominis is the only one currently described involving two obligate human mucosal symbionts with pathogenic capabilities that can cause independent diseases in the same anatomical site: the lower urogenital tract. Although several aspects of this intriguing microbial partnership have been investigated, many questions on the influence of this symbiosis on the parasite pathobiology still remain unanswered. Here, we examined with in vitro cultures how M. hominis could influence the pathobiology of T. vaginalis by investigating the influence of M. hominis on parasite replication rate, haemolytic activity and ATP production. By comparing isogenic mycoplasma-free T. vaginalis and parasites stably associated with M. hominis we could demonstrate that the latter show a higher replication rate, increased haemolytic activity and are able to produce larger amounts of ATP. In addition, we demonstrated in a T. vaginalis-macrophage co-culture system that M. hominis could modulate an aspect of the innate immuno-response to T. vaginalis infections by influencing the production of nitric oxide (NO) by human macrophages, with the parasite-bacteria symbiosis outcompeting the human cells for the key substrate arginine. These results support a model in which the symbiosis between T. vaginalis and M. hominis influences host-microbes interactions to the benefit of both microbial partners during infections and to the detriment of their host. PMID:27379081

  8. Comparison of commercially available media for detection and isolation of Ureaplasma urealyticum and Mycoplasma hominis.

    Science.gov (United States)

    Broitman, N L; Floyd, C M; Johnson, C A; de la Maza, L M; Peterson, E M

    1992-05-01

    The Mycotrim Triphasic flask system (Irvine Scientific, Irvine, Calif.) was compared with a system composed of Mycotrim GU broth (Irvine Scientific) and A7 or A8 agar (Remel, Lenexa, Kans.) for the ability to detect Ureaplasma urealyticum and Mycoplasma hominis from 129 genital specimens. Of the 64 specimens positive for U. urealyticum, 25, 98, and 100% were detected on Mycotrim Triphasic agar and A7 and A8 agars, respectively. All 18 specimens that grew M. hominis were detected by A7 and A8 agars, and 94% grew on Mycotrim Triphasic agar. Mycotrim GU broth detected all of the positive specimens, and Mycotrim Triphasic broth detected all but one. Mycotrim GU broth inoculated simultaneously with either A7 or A8 agar was found to be more sensitive and cost-effective than the Mycotrim Triphasic flask system.

  9. Case report: cutaneous myiasis caused by Dermatobia hominis, the human botfly.

    Science.gov (United States)

    Garvin, Kanishka W; Singh, Virtaj

    2007-05-01

    Cutaneous myiasis caused by Dermatobia hominis, the human botfly, involves the infestation of human tissue with fly larvae, and is common in Central and South America. We report a case of a 57-year-old man with cutaneous myiasis imported into the US from Belize. The epidemiology, biological life cycle, clinical presentation, and various methods of larval extraction, including incision and drainage, are discussed.

  10. "Rate of Chlamydia trachomatis, Mycoplasma hominis and Ureaplasma urealyticum in Infertile Females and Control Group"

    Directory of Open Access Journals (Sweden)

    N Badami

    2001-07-01

    Full Text Available Infertility in famale is one of the most important sequela of genital infection with Chlamydia trachomatis, Mycoplasma hominis and Ureaplasma urealyticum. In the present study the frequency of these bacteries was studied in 125 infertile female by direct and indirect immunofluorscence tests and culture method and compared with 250 normal population. Mycoplasma hominis was isolated from 32 (35.6% of infertile females compare with 18 (7.2% of normal population. Ureaplasma urealyticum was isolated from 41 (32.8% of infertile females compare to 48 (19.2% of normal population. Chlamydia trachomatis was detected by direct IF in 11 (8.8% of infertile and 2 (0.8% control group. The antibody titer against D-K serotypes of Chlamydia trachomatis was also measured in both groups of infertile and normal population and a positive titer of 1/16 and above was detected in 26 (20.8% of infertile cases and in 8 (3.2% of control group. The rate of Chlamydia trachomatis, Mycoplasma hominis and Ureaplasma urealyticum in case and control groups was significant (respectively P<0.0001, P<0.0001, p= 0.0018.

  11. Prevalence and genetic diversity of the intestinal parasites Blastocystis sp. and Cryptosporidium spp. in household dogs in France and evaluation of zoonotic transmission risk.

    Science.gov (United States)

    Osman, Marwan; Bories, Jessica; El Safadi, Dima; Poirel, Marie-Thérèse; Gantois, Nausicaa; Benamrouz-Vanneste, Sadia; Delhaes, Laurence; Hugonnard, Marine; Certad, Gabriela; Zenner, Lionel; Viscogliosi, Eric

    2015-11-30

    Several parasites including the protozoa Blastocystis sp. and Cryptosporidium spp. may be causative agents of gastrointestinal symptoms in domestic dogs, and there may be a potential risk of transmission to owners. While France is one of the largest European countries in terms of its canine population, little data is available about the molecular epidemiology of these two parasites. The purpose of this study was to determine the prevalence of intestinal parasites in household dogs in France, and to evaluate the zoonotic risk of Blastocystis sp. and Cryptosporidium spp. by genotyping the corresponding isolates. To this end, 116 faecal samples were collected from household dogs regardless of breed, age or gender, living in the Lyons area, France. Various intestinal protozoa and helminths were identified by light microscopy. Screening for Blastocystis sp. and Cryptosporidium spp. were subsequently performed by PCR targeting the small subunit (SSU) rDNA coding region, followed by direct sequencing of the PCR products and analysis of the sequences obtained for genotyping. The overall prevalence of dogs infected with at least one gastrointestinal parasite was 42.2% (49/116). After light microscopy examination of faecal samples, the most common parasites found were the protozoa Giardia sp. (25.0%) and Cystoisospora sp. (19.8%). Using molecular methods, four dogs (3.4%) were shown to be infected by Blastocystis sp. and carried either subtype (ST) 2, commonly identified in various animal groups, or ST10, frequently found in bovids. Three dogs (2.6%) were positive for C. canis, infecting humans episodically. The low prevalence of both parasites, combined with the identification of C. canis and Blastocystis sp. ST2 and ST10 in the canine population, strongly suggests that dogs play a negligible role as zoonotic reservoirs for both parasites and do not seem to be natural hosts of Blastocystis sp.

  12. High rates of double-stranded RNA viruses and Mycoplasma hominis in Trichomonas vaginalis clinical isolates in South Brazil.

    Science.gov (United States)

    da Luz Becker, Débora; dos Santos, Odelta; Frasson, Amanda Piccoli; de Vargas Rigo, Graziela; Macedo, Alexandre José; Tasca, Tiana

    2015-08-01

    Trichomonas vaginalis is the etiological agent of trichomoniasis, the most common non-viral sexually transmitted disease (STD) in world, with 276.4 million new cases each year. T. vaginalis can be naturally infected with Mycoplasma hominis and Trichomonasvirus species. This study aimed to evaluate the prevalence of T. vaginalis infected with four distinct T. vaginalis viruses (TVVs) and M. hominis among isolates from patients in Porto Alegre city, South Brazil. An additional goal of this study was to investigate whether there is association between metronidazole resistance and the presence of M. hominis during TVV infection. The RNA expression level of the pyruvate ferredoxin oxidoreductase (PFOR) gene was also evaluated among metronidazole-resistant and metronidazole-sensitive T. vaginalis isolates. A total of 530 urine samples were evaluated, and 5.7% samples were positive for T. vaginalis infection. Among them, 4.51% were isolated from female patients and 1.12% were from male patients. Remarkably, the prevalence rates of M. hominis and TVV-positive T. vaginalis isolates were 56.7% and 90%, respectively. Most of the T. vaginalis isolates were metronidazole-sensitive (86.7%), and only four isolates (13.3%) were resistant. There is no statistically significant association between infection by M. hominis and infection by TVVs. Our results refute the hypothesis that the presence of the M. hominis and TVVs is enough to confer metronidazole resistance to T. vaginalis isolates. Additionally, the role of PFOR RNA expression levels in metronidazole resistance as the main mechanism of resistance to metronidazole could not be established. This study is the first report of the T. vaginalis infection by M. hominis and TVVs in a large collection of isolates from South Brazil.

  13. Antiprotozoal effects of Oregano oil and Brucea javanica on Blastocystis hominis in vitro%中药牛至油、鸦胆子对人芽囊原虫的体外杀灭作用

    Institute of Scientific and Technical Information of China (English)

    乔继英; 李利军; 张荣; 张旭; 蔡娟; 杨华

    2008-01-01

    目的 通过观察牛至油、鸦胆子对人芽囊原虫的体外杀灭作用,筛选治疗人芽囊原虫感染有效药物及浓度.方法 从腹泻患者新鲜粪便中分离人芽囊原虫,分别用含牛至油和鸦胆子1640培养基培养,药物设200、400、800、1 600 、3 200和6 400 μg/ml组.于加药24 h和 72 h作虫体计数,评价药物效果,试验设甲硝唑药物对照组和不加药空白对照组.结果 牛至油1 600、3 200 μg/ml作用72 h、6 400 μg/ml作用24 h,虫体全部死亡,最适杀虫体浓度为800 μg/ml;鸦胆子6 400 μg/ml 24 h虫体全部死亡,最适杀虫浓度为1 600~3 200 μg/ml;甲硝唑40 μg/ml 72 h、≥80 μg/ml 24 h虫体全部死亡.结论 牛至油和鸦胆子具有体外杀灭人芽囊原虫作用.且牛至油的杀虫效果强于鸦胆子.

  14. Genome Anatomy of Pyrenochaeta unguis-hominis UM 256, a Multidrug Resistant Strain Isolated from Skin Scraping.

    Science.gov (United States)

    Toh, Yue Fen; Yew, Su Mei; Chan, Chai Ling; Na, Shiang Ling; Lee, Kok Wei; Hoh, Chee-Choong; Yee, Wai-Yan; Ng, Kee Peng; Kuan, Chee Sian

    2016-01-01

    Pyrenochaeta unguis-hominis is a rare human pathogen that causes infection in human skin and nail. P. unguis-hominis has received little attention, and thus, the basic biology and pathogenicity of this fungus is not fully understood. In this study, we performed in-depth analysis of the P. unguis-hominis UM 256 genome that was isolated from the skin scraping of a dermatitis patient. The isolate was identified to species level using a comprehensive multilocus phylogenetic analysis of the genus Pyrenochaeta. The assembled UM 256 genome has a size of 35.5 Mb and encodes 12,545 putative genes, and 0.34% of the assembled genome is predicted transposable elements. Its genomic features propose that the fungus is a heterothallic fungus that encodes a wide array of plant cell wall degrading enzymes, peptidases, and secondary metabolite biosynthetic enzymes. Antifungal drug resistance genes including MDR, CDR, and ERG11/CYP51 were identified in P. unguis-hominis UM 256, which may confer resistance to this fungus. The genome analysis of P. unguis-hominis provides an insight into molecular and genetic basis of the fungal lifestyles, understanding the unrevealed biology of antifungal resistance in this fungus.

  15. Genome Anatomy of Pyrenochaeta unguis-hominis UM 256, a Multidrug Resistant Strain Isolated from Skin Scraping

    Science.gov (United States)

    Toh, Yue Fen; Yew, Su Mei; Chan, Chai Ling; Na, Shiang Ling; Lee, Kok Wei; Hoh, Chee-Choong; Yee, Wai-Yan; Ng, Kee Peng

    2016-01-01

    Pyrenochaeta unguis-hominis is a rare human pathogen that causes infection in human skin and nail. P. unguis-hominis has received little attention, and thus, the basic biology and pathogenicity of this fungus is not fully understood. In this study, we performed in-depth analysis of the P. unguis-hominis UM 256 genome that was isolated from the skin scraping of a dermatitis patient. The isolate was identified to species level using a comprehensive multilocus phylogenetic analysis of the genus Pyrenochaeta. The assembled UM 256 genome has a size of 35.5 Mb and encodes 12,545 putative genes, and 0.34% of the assembled genome is predicted transposable elements. Its genomic features propose that the fungus is a heterothallic fungus that encodes a wide array of plant cell wall degrading enzymes, peptidases, and secondary metabolite biosynthetic enzymes. Antifungal drug resistance genes including MDR, CDR, and ERG11/CYP51 were identified in P. unguis-hominis UM 256, which may confer resistance to this fungus. The genome analysis of P. unguis-hominis provides an insight into molecular and genetic basis of the fungal lifestyles, understanding the unrevealed biology of antifungal resistance in this fungus. PMID:27626635

  16. Genetic Passive Immunization with Adenoviral Vector Expressing Chimeric Nanobody-Fc Molecules as Therapy for Genital Infection Caused by Mycoplasma hominis.

    Directory of Open Access Journals (Sweden)

    Daria A Burmistrova

    Full Text Available Developing pathogen-specific recombinant antibody fragments (especially nanobodies is a very promising strategy for the treatment of infectious disease. Nanobodies have great potential for gene therapy application due to their single-gene nature. Historically, Mycoplasma hominis has not been considered pathogenic bacteria due to the lack of acute infection and partially due to multiple studies demonstrating high frequency of isolation of M. hominis samples from asymptomatic patients. However, recent studies on the role of latent M. hominis infection in oncologic transformation, especially prostate cancer, and reports that M. hominis infects Trichomonas and confers antibiotic resistance to Trichomonas, have generated new interest in this field. In the present study we have generated specific nanobody against M. hominis (aMh, for which the identified target is the ABC-transporter substrate-binding protein. aMh exhibits specific antibacterial action against M. hominis. In an attempt to improve the therapeutic properties, we have developed the adenoviral vector-based gene therapy approach for passive immunization with nanobodies against M. hominis. For better penetration into the mucous layer of the genital tract, we fused aMh with the Fc-fragment of IgG. Application of this comprehensive approach with a single systemic administration of recombinant adenovirus expressing aMh-Fc demonstrated both prophylactic and therapeutic effects in a mouse model of genital M. hominis infection.

  17. Revisiting the reference genomes of human pathogenic Cryptosporidium species: reannotation of C. parvum Iowa and a new C. hominis reference

    Science.gov (United States)

    Isaza, Juan P.; Galván, Ana Luz; Polanco, Victor; Huang, Bernice; Matveyev, Andrey V.; Serrano, Myrna G.; Manque, Patricio; Buck, Gregory A.; Alzate, Juan F.

    2015-01-01

    Cryptosporidium parvum and C. hominis are the most relevant species of this genus for human health. Both cause a self-limiting diarrhea in immunocompetent individuals, but cause potentially life-threatening disease in the immunocompromised. Despite the importance of these pathogens, only one reference genome of each has been analyzed and published. These two reference genomes were sequenced using automated capillary sequencing; as of yet, no next generation sequencing technology has been applied to improve their assemblies and annotations. For C. hominis, the main challenge that prevents a larger number of genomes to be sequenced is its resistance to axenic culture. In the present study, we employed next generation technology to analyse the genomic DNA and RNA to generate a new reference genome sequence of a C. hominis strain isolated directly from human stool and a new genome annotation of the C. parvum Iowa reference genome. PMID:26549794

  18. Selection of Mycoplasma hominis PG21 deletion mutants by cultivation in the presence of monoclonal antibody 552

    DEFF Research Database (Denmark)

    Jensen, Lise Torp; Ladefoged, Søren; Birkelund, Svend

    1995-01-01

    Three mutants of Mycoplasma hominis PG21 were isolated and shown to contain alterations in the size of a repeat-containing gene encoding a surface-localized 135-kDa antigen designated Lmp1. The mutants were isolated by cultivating M. hominis for a 3-month period in the presence of Lmp1-specific...... characterized. The mutants showed deletions of a various number of repeats. The deletions were accompanied by a decrease in size of the proteins. With increasing size of deletions, agglutination and growth inhibition by MAb 552 became less pronounced. Spontaneous aggregation of the mutant M. hominis cells...... in culture medium was, however, increased, indicating that the repeated elements may be of importance for repulsion of the cells....

  19. Comparison of direct fecal smear microscopy, culture, and polymerase chain reaction for the detection ofBlastocystis sp. in human stool samples

    Institute of Scientific and Technical Information of China (English)

    Herbert J Santos; Windell L Rivera

    2013-01-01

    Objective:To compare the sensitivity and specificity of direct fecal smear microscopy, culture, and polymerase chain reaction in the detection ofBlastocystis sp. in human stool. Methods:Human stool samples were collected from a community inSanIsidro,Rodriguez, Rizal,Philippines.These samples were subjected to direct fecal smear microscopy, culture and polymerase chain reaction to detect the presence of Blastocystissp.Results:Of the110 stool samples collected,28(25%) were detected positive for the presence ofBlastocystis sp. by two or more tests.Culture method detected the highest number ofBlastocystis-positive stool samples (n=36), followed byPCR ofDNA extracted from culture(n=26),PCR ofDNA extracted from stool (n=10), and direct fecal smear(n=9).Compared to culture, the sensitivity of the other detection methods were66.7% forPCR from culture and19.4% for bothPCR from stool and direct fecal smear.Specificity of the methods was high, withPCR from culture and direct fecal smear having 97.3%, whilePCR from stool at95.9%.Conclusions:In this study,in vitroculture is the best method for detectingBlastocystis sp. in human stool samples.

  20. Concurrent infections with Cryptosporidium spp., Giardia duodenalis, Enterocytozoon bieneusi, and Blastocystis spp. in naturally infected dairy cattle from birth to two years of age

    Science.gov (United States)

    Fecal specimens were collected directly at weekly and then monthly intervals from each of 30 dairy calves from birth to 24 months to determine the prevalence and age distribution of Cryptosporidium spp., Giardia duodenalis assemblages, Enterocytozoon bieneusi genotypes, and Blastocystis spp subtypes...

  1. Centriole behaviour during meiosis of male germ cells of Dermatobia hominis (Diptera:Cuterebridae).

    Science.gov (United States)

    Quagio-Grassiotto, I; de Lello, E

    1996-01-01

    During the meiotic division of Dermatobia hominis spermatogenesis, the centrioles duplicate only in prophase I, giving rise to short cilia which are exposed on the cellular surface. In metaphase I they are internalized and distributed to the daughter cells. Consequently, the secondary spermatocytes have two centrioles which repeat the cycle of cilia externalization followed by internalization. The spermatids receive only one centriole, which changes into a basal body and originates a flagellum. This centriole behaviour seems to be a general feature in insect male germ cell meiosis.

  2. Myiasis Dermatobia hominis, Linn: report of a case and review of the literature.

    Science.gov (United States)

    Rosen, I J; Neuberger, D

    1977-01-01

    A description of the life cycle of the tropical botfly, Dermatobia hominis, is presented. The entomology of this parasite is discussed along with the pathologic manifestations of human infestation and a description of the various modes of treatment. A recent case of multiple botfly infestation in a young man during a trip to Central America is reported. A review of the literature including the earliest reported case (1904) is presented, along with a brief discussion of the epidemiology and public health aspects of this problem.

  3. THE METHODS OF LABORATORY DIAGNOSTICS OF UROGENITAL INFECTIONS ASSOCIATED WITH MYCOPLASMA HOMINIS AND UREAPLASMA SPP.

    Directory of Open Access Journals (Sweden)

    O. V. Zarucheynova

    2014-01-01

    Full Text Available Wide distribution of urogenital mycoplasmas in the population, the high frequency of carrier state and a long asymptomatic course of disease, the lack of specific clinical symptoms making the diagnosis impossible without using of special laboratory tests. The review focuses on indications for mycoplasma infection screening and for an appointmentof antibiotic therapy. The most commonly used laboratory diagnostic methods of urogenital infections, associated with Mycoplasma hominis and Ureaplasma spp., with their characteristics, advantages and disadvantages are described.

  4. Morphology of human Fallopian tupes after infection with Mycoplasma genitalium and Mycoplasma hominis - in vitro organ culture study

    DEFF Research Database (Denmark)

    Baczynska, Agata; Funch, P.; Fedder, J.;

    2006-01-01

    that mycoplasmas can cause tubal factor infertility. We analysed the effects of infections with Mycoplasma hominis and Mycoplasma genitalium on the HFT epithelium and compared them with the effects of infections with genital pathogens: Chlamydia trachomatis and Neisseria gonorrhoeae. METHODS We used an in vitro......-terminal part of the adhesion protein MgPa before infection of HFT organ culture. CONCLUSION We have shown that the presence of M. genitalium, but not M. hominis, in the HFT organ culture affected the epithelium and resulted in cilia damage. The effect of infection with M. genitalium on the HFT was, however...

  5. Morphology of human Fallopian tubes after infection with Mycoplasma genitalium and Mycoplasma hominis--in vitro organ culture study

    DEFF Research Database (Denmark)

    Baczynska, Agata; Funch, P; Fedder, J;

    2007-01-01

    that mycoplasmas can cause tubal factor infertility. We analysed the effects of infections with Mycoplasma hominis and Mycoplasma genitalium on the HFT epithelium and compared them with the effects of infections with genital pathogens: Chlamydia trachomatis and Neisseria gonorrhoeae. METHODS: We used an in vitro...... against the C-terminal part of the adhesion protein MgPa before infection of HFT organ culture. CONCLUSION: We have shown that the presence of M. genitalium, but not M. hominis, in the HFT organ culture affected the epithelium and resulted in cilia damage. The effect of infection with M. genitalium...

  6. Scanning electron microscopy studies on the first-instar larva of Dermatobia hominis.

    Science.gov (United States)

    de Filippis, T; Leite, A C

    1997-04-01

    First-instar larvae of Dermatobia hominis collected 1, 4 and 7 days after having penetrated experimentally infected rats, were studied by scanning electron microscope (SEM) observation. On the pseudocephalon there are basiconic and trichoid sensilla (antennal sensory complex), and basiconic, coeloconic and campaniform sensilla (maxillary sensory complex). The thoracic segments bear several rows of small, backwardly pointed, spines, and trichoid, campaniform, coeloconic and pit sensilla. The anterior spiracle is a minute opening. Both small and large spines directed posteriorly are on the first to fourth abdominal segments, which also bear coeloconic and companiform sensilla. These sensilla are present on the unarmed (fifth and sixth) and armed (seventh) abdominal segments. The seventh and the last (eight) abdominal segments have forwardly directed spines. Each spiracular plate has two spiracular openings and four spatulate-like structures called sun rays. The anus and the coeloconic sensilla are proeminent on the last segment. The results are compared with other parasitic dipteran larvae, and emphasize that the multiple types of sensilla on D.hominis larva may have importance in establishing the parasitic phase of the life cycle of this insect.

  7. Genotypic Characterization of Cryptosporidium hominis from Water Samples in São Paulo, Brazil

    Science.gov (United States)

    Araújo, Ronalda S.; Dropa, Milena; Fernandes, Licia N.; Carvalho, Terezinha T.; Sato, Maria Inês Z.; Soares, Rodrigo M.; Matté, Glavur R.; Matté, Maria Helena

    2011-01-01

    The protozoan parasite Cryptosporidium has emerged as one of the most important water contaminants, causing waterborne outbreaks of diarrheal diseases worldwide. The small size of oocysts under the microscope and the possibility of changes in characteristics of oocysts, mainly in environmental samples, make the taxonomy of the genus difficult if morphologic characteristics are considered. This limitation encouraged the application of molecular methods to identify this microorganism. The aim of this study was to detect and identify by nested-polymerase chain reaction oocysts of Cryptosporidium present in water samples in the state of São Paulo, Brazil. Water samples were concentrated through a membrane filter, DNA was extracted by using a standard technique, and both amplification reactions used forward and reverse oligonucleotides that were complementary to Cryptosporidium 18S ribosomal RNA gene sequences. Thirty water samples from different sites of collection in the state of São Paulo were evaluated. Cryptosporidium oocysts were detected in 30% of the samples. By genoptyping, C. hominis and Cryptosporidium sp. were identified in recreational water and C. meleagridis was identified in surface water samples. This is the first report of C. hominis in environmental samples in Brazil. Although identification of Cryptosporidium is still a difficult task, molecular methods are essential for specific identification and are a helpful tool to aid to understand the epidemiology of this parasite in Brazil. PMID:22049036

  8. Selection of Mycoplasma hominis PG21 deletion mutants by cultivation in the presence of monoclonal antibody 552

    DEFF Research Database (Denmark)

    Jensen, L T; Ladefoged, S; Birkelund, S;

    1995-01-01

    characterized. The mutants showed deletions of a various number of repeats. The deletions were accompanied by a decrease in size of the proteins. With increasing size of deletions, agglutination and growth inhibition by MAb 552 became less pronounced. Spontaneous aggregation of the mutant M. hominis cells...

  9. Ureaplasma urealyticum, Ureaplasma parvum, Mycoplasma hominis and Mycoplasma genitalium infections and semen quality of infertile men

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    Rebai Tarek

    2007-11-01

    Full Text Available Abstract Background Genital ureaplasmas (Ureaplasma urealyticum and Ureaplasma parvum and mycoplasmas (Mycoplasma genitalium and Mycoplasma hominis are potentially pathogenic species playing an etiologic role in both genital infections and male infertility. Reports are, however, controversial regarding the effects of these microorganisms infections in the sperm seminological variables. This study aimed at determining the frequency of genital ureplasmas and mycoplasmas in semen specimens collected from infertile men, and at comparing the seminological variables of semen from infected and non-infected men with these microorganisms. Methods A total of 120 semen samples collected from infertile men were investigated. Semen specimens were examined by in-house PCR-microtiter plate hybridization assay for the presence of genital ureaplasmas and mycoplasmas DNA. Semen analysis was assessed according to the guidelines of the World Health Organization. Standard parametric techniques (t-tests and nonparametric techniques (Wilcoxon tests were used for statistical analysis. Results The frequency of genital ureaplasmas and mycoplasmas detected in semen samples of infertile men was respectively 19.2% (23/120 and 15.8% (19/120. The frequency of Ureaplasma urealyticum (15% was higher than that of Mycoplasma hominis (10.8%, Ureaplasma parvum (4.2% and Mycoplasma genitalium (5%. Mixed species of mycoplasmas and ureaplasmas were detected in 6.7% of semen samples. Comparison of the parameters of the standard semen analysis between the male partners of the infertile couples with and without genital ureaplasmas and mycoplasmas infection showed that the presence of Mycoplasma hominis DNA in semen samples is associated with low sperm concentration (p = 0.007 and abnormal sperm morphology (p = 0.03 and a negative correlation between sperm concentration and the detection of Mycoplasma genitalium in semen samples of infertile men (p = 0.05. The mean values of seminal

  10. P80, the HinT interacting membrane protein, is a secreted antigen of Mycoplasma hominis

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    Henrich Birgit

    2004-12-01

    Full Text Available Abstract Background Mycoplasmas are cell wall-less bacteria which encode a minimal set of proteins. In Mycoplasma hominis, the genes encoding the surface-localized membrane complex P60/P80 are in an operon with a gene encoding a cytoplasmic, nucleotide-binding protein with a characteristic Histidine triad motif (HinT. HinT is found in both procaryotes and eukaryotes and known to hydrolyze adenosine nucleotides in eukaryotes. Immuno-precipitation and BIACore analysis revealed an interaction between HinT and the P80 domain of the membrane complex. As the membrane anchored P80 carries an N-terminal uncleaved signal peptide we have proposed that the N-terminus extends into the cytoplasm and interacts with the cytosolic HinT. Results Further characterization of P80 suggested that the 4.7 kDa signal peptide is protected from cleavage only in the membrane bound form. We found several proteins were released into the supernatant of a logarithmic phase mycoplasma culture, including P80, which was reduced in size by 10 kDa. Western blot analysis of recombinant P80 mutants expressed in E. coli and differing in the N-terminal region revealed that mutation of the +1 position of the mature protein (Asn to Pro which is important for signal peptidase I recognition resulted in reduced P80 secretion. All other P80 variants were released into the supernatant, in general as a 74 kDa protein encompassing the helical part of P80. Incubation of M. hominis cells in phosphate buffered saline supplemented with divalent cations revealed that the release of mycoplasma proteins into the supernatant was inhibited by high concentrations of calciumions. Conclusions Our model for secretion of the P80 protein of M. hominis implies a two-step process. In general the P80 protein is transported across the membrane and remains complexed to P60, surface-exposed and membrane anchored via the uncleaved signal sequence. Loss of the 4.7 kDa signal peptide seems to be a pre-requisite for P

  11. Attempts to culture the parasitic stage of Dermatobia hominis (L. Jr.) in vitro (Diptera: Cuterebridae).

    Science.gov (United States)

    Zeledón, R; Silva, S

    1987-10-01

    Dermatobia hominis larvae were cultured in a semidefined liquid medium. First-instar larvae (L1) grew well up to 44 days; 29.1% molted in a mean period of 8.62 days. Two larvae reached the third instar but lived only 1 and 18 days, respectively, after the second molt. The increase in size, measured in 4 larvae, was about 10-fold. Second- and third-instar larvae, obtained from the skin of cattle, survived and grew in the medium for up to 2 mo; 39.0% of the L2 molted while 77.3% of the L3 pupated, and some produced flies when transferred to sand after 14.84 +/- 10.08 days in the culture medium. Some maturation factor, obtained from the skin, may be necessary for the larvae to grow satisfactorily and to complete the full parasitic cycle in vitro.

  12. Clinical Review of the Effects of Hominis Placental Pharmacopuncture in the Treatment of Facial Spasm Patients

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    Jo Na-Young

    2013-09-01

    Full Text Available Objectives: The main purpose of this research is to investigate the effect of treatment with Hominis Placental pharmacopuncture (HPP for 32 patients with hemifacial spasm. Methods: We treated facial spasm patients with acupuncture and HPP at Sabaek (ST2, Seung-eup (ST1, Gwallyeo (SI18, Chanjuk (BL2, Sajukgong (TE23, Hagwan (ST7, Hyeopgeo (ST6, Jichang (ST4, Wan-gol (SI4 and Yepung (TE17, and we investigated the effect by using Scott’s scale. The data were analyzed by using the SPSS/10.0 for windows program with descriptive statistics, the paired t-test, and the Shapiro-Wilk normality test. Results: After treatment, the grade of the spasm’s intensity based on Scott’s description were decreased significantly. About 72% of the patients felt that the combination treatment had produced excellent results. Conclusion: These data suggested that HPP can be useful for treating facial spasm patients.

  13. Analysis of the Complete Mycoplasma hominis LBD-4 Genome Sequence Reveals Strain-Variable Prophage Insertion and Distinctive Repeat-Containing Surface Protein Arrangements

    OpenAIRE

    2015-01-01

    The complete genome sequence of Mycoplasma hominis LBD-4 has been determined and the gene content ascribed. The 715,165-bp chromosome contains 620 genes, including 14 carried by a strain-variable prophage genome related to Mycoplasma fermentans MFV-1 and Mycoplasma arthritidis MAV-1. Comparative analysis with the genome of M. hominis PG21T reveals distinctive arrangements of repeat-containing surface proteins.

  14. Analysis of the Complete Mycoplasma hominis LBD-4 Genome Sequence Reveals Strain-Variable Prophage Insertion and Distinctive Repeat-Containing Surface Protein Arrangements.

    Science.gov (United States)

    Calcutt, Michael J; Foecking, Mark F

    2015-02-26

    The complete genome sequence of Mycoplasma hominis LBD-4 has been determined and the gene content ascribed. The 715,165-bp chromosome contains 620 genes, including 14 carried by a strain-variable prophage genome related to Mycoplasma fermentans MFV-1 and Mycoplasma arthritidis MAV-1. Comparative analysis with the genome of M. hominis PG21(T) reveals distinctive arrangements of repeat-containing surface proteins.

  15. Phylogeny of some mycoplasmas from ruminants based on 16S rRNA sequences and definition of a new cluster within the hominis group.

    Science.gov (United States)

    Pettersson, B; Uhlén, M; Johansson, K E

    1996-10-01

    Almost complete (> 96%) 16S rRNA sequences from nine ruminant mycoplasmas have been determined by solid-phase DNA sequencing. Polymorphisms were found in four of the 16S rRNA sequences, which indicated the existence of two different rRNA operons. Seven polymorphisms were found in Mycoplasma agalatiae, three were found in Mycoplasma bovis, one was found in Mycoplasma alkalescens, and one was found in Mycoplasma bovirhinis. The sequence data were used for construction of phylogenetic trees. All but one of the ruminant mycoplasmas sequenced in this work clustered in the hominis group. A close relationship was found between M. agalactiae and M. bovis, with a 99% nucleotide similarity between their 16S rRNA sequences. They were also found to be members of the Mycoplasma lipophilum cluster of the hominis group. Furthermore, the 16S rRNA comparisons showed that Mycoplasma alkalescens and Mycoplasma canadense are closely related (> 98.5%), and these species were found to cluster in the Mycoplasma hominis cluster of the hominis group. Interestingly, M. bovirhinis grouped in a new phylogenetic cluster of the hominis group. The new cluster, which was supported by bootstrap percentage values, signature nucleotide analysis, and higher-order structural elements, was named the Mycoplasma synoviae cluster. Mycoplasma bovoculi, Mycoplasma conjunctivae, and Mycoplasma ovipneumoniae clustered in the Mycoplasma neurolyticum cluster of the hominis group. Mycoplasma alvi clustered with Mycoplasma pirum in the M. pneumoniae cluster of the pneumoniae group.

  16. Fatal nosocomial meningitis caused by Mycoplasma hominis in an adult patient: case report and review of the literature

    Directory of Open Access Journals (Sweden)

    Sophie Reissier

    2016-07-01

    Full Text Available Meningitis due to Mycoplasma hominis in adults is rarely described, with only three cases having been reported to date. A case of fatal meningitis in a 39-year-old patient after a neurosurgical procedure for a subarachnoid haemorrhage is reported herein. Identification and treatment were significantly delayed because of the rarity of the aetiology and difficulty identifying this organism with the routinely used conventional methods, such as Gram staining and agar growth on standard agar plates. Clinical procedures and the treatment of ‘culture-negative’ central nervous system infections is a real challenge for clinical microbiologists and clinicians, and M. hominis has to be considered as a potential, although very uncommon, pathogen.

  17. A Clinical Study on 1 Case of Patient with Bilateral Simultaneous Bell's Palsy Treated by Hominis Placenta Herbal-Acupuncture

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    Kwon, Kang

    2003-06-01

    Full Text Available Objective : This study was carried out to investigate the progress of bilateral simultaneous facial palsy and the effect of Hominis Placenta herbal-acupunture and the other oriental medical therapies. Methods : We used two methods to research the progress of disease. 1. Diagnosis - Facial muscle test, Taste test, Hearing test, Photographies, Lab-finding 2. Treatment - Acupuncture, Herbal-acupuncture, Electroacupuncture, Herb-med Results : The onset of Rt. facial palsy was earlier than Lt. facial palsy 3days. The reaction on the treatment of Rt. facial palsy was more dull than Lt. facial palsy. In terms of treatment period, Rt. facial palsy was very longer than Lt. facial palsy. Conclusion : According to the above results, we discoveried that Hominis Placenta herbal-acupunture and the other oriental medical therapies had good influence on the bilateral simultaneous facial palsy. In the future, we should endeavor to know influence between Rt. and Lt. face in case of bilateral simultaneous Bell's palsy.

  18. A Clinical Study on 1 Case of Patient with Bilateral Simultaneous Bell's Palsy Treated by Hominis Placenta Herbal-Acupuncture

    OpenAIRE

    2003-01-01

    Objective : This study was carried out to investigate the progress of bilateral simultaneous facial palsy and the effect of Hominis Placenta herbal-acupunture and the other oriental medical therapies. Methods : We used two methods to research the progress of disease. 1. Diagnosis - Facial muscle test, Taste test, Hearing test, Photographies, Lab-finding 2. Treatment - Acupuncture, Herbal-acupuncture, Electroacupuncture, Herb-med Results : The onset of Rt. facial palsy was earlier ...

  19. Effect of Hominis placenta Pharmacopuncture on the Dysmenorrhea (A Pilot study, Single blind, Randomized, Controlled Clinical Trial

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    Su-Min Kim

    2008-09-01

    Full Text Available Objective : This study was designed to evaluate the effect of Hominis placenta Pharmacopuncture treatment on Dysmenorrhea of Women. Methods : 49 subjects who were suffering from dysmenorrhea volunteered to answer the MMP(Measure of Menstrual Pain and MSSL(Menstrual Symptom Severity List questionnaire. They were divided into two groups, a Hominis placenta Pharmacopuncture treatment group(Experiment al group, n=25 and a Normal Saline(N/S treatment group(Control group, n=24. The two groups were injected on the CV4, S36, Sp9 and Sp6 acupuncture point. They were treated totally five times depending on the individual menstruation cycles. The scores of MMP and MSSL were measured overall three times before and after the menstruation cycle. The collected data were analyzed as paired t-test, independent t-test using SPSS 12.0 WIN Program. Results : As a result of the evaluation by MMP and MSSL, a significant improvement on dysmenorrhea was made in the two groups(p<0.05, and both scores of Experiment group were decreased more than Control group. But there was no significant difference between the two groups. Conclusions : The Hominis placenta Pharmacopuncture treatment and the Normal Saline treatment were effective in decreasing the symptom of Dysmenorrhea.

  20. Studies in vitro on the relative efficacy of current acaricides for Sarcoptes scabiei var. hominis.

    Science.gov (United States)

    Walton, S F; Myerscough, M R; Currie, B J

    2000-01-01

    Resistance of Sarcoptes scabiei to various topical therapies has been described, but clinical assessment of treatment failure is problematic and in-vitro assays are generally not available. We describe a simple in-vitro analysis used to evaluate the relative efficacy of a range of topical, oral, and herbal treatments available in Australia for the treatment of scabies. S. scabiei var. hominis mites were collected from skin scrapings obtained from 7 crusted scabies patients over a period of 2 years (1997 and 1998). Larvae, nymphal instars, and adult mites were tested within 3 h of collection and continuously exposed to selected commercially available treatment products until death, with the elapsed time recorded. Neem was the only product to show little acaricidal activity. Survival curves indicated that, of the other agents, 5% permethrin (Lyclear) had the slowest killing time, with 35% of mites still alive after 3 h, and 4% still alive after 18-22 h of constant exposure. In contrast, no mites were alive after 3 h exposure to 25% benzyl benzoate (Ascabiol), 1% lindane (Quellada), 5% tea tree oil and 100-8000 ng/g of ivermectin (Equimec). Despite the slower killing time with 5% permethrin, there was no evidence of any mite tolerance in vivo or treatment failure in any patients or contact cases.

  1. Therapeutic effects of Hominis placenta herb-acupuncture in adjuvant-induced arthritis rat

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    MiJung Yeom

    2002-02-01

    Full Text Available Rheumatoid arthritis (RA is a chronic inflammatory autoimmune disease, characterized by leukocyte infiltration, a chronic inflammation of the joint, a pannus formation and the extensive destruction of the articular cartilage and bone. Several proinflammatory cytokines such as tumor necrosis factor-α (TNF-α, interleukin-1β (IL-1β and interleukin 6 (IL-6 have been implicated in the pathological mechanisms of synovial tissue proliferation, joint destruction and programmed cell death in rheumatoid joint. In the Korean traditional medicine, Hominis placenta (HP as an herbal solution of herb-acupuncture has been widely used to treat the inflammatory diseases including RA. In order to study the medicinal effect of HP herb-acupuncture on rheumatoid joint, an adjuvant-induced arthritis (AIA was generated by the injection of 1.5 mg of Mycobacterium tuberculosis, emulsified in squalene, to the base of the tail of Spraque-Dawley (SD rats. After onset stage of polyarthritis, HP was daily injected to the Zusanli (ST36 acupuncture points in both of rat lags and the expression patterns of cytokines such as TNF-α, IL-1β, and IL-6 at the knee joint were analyzed using immunostaining and RT-PCR. The HP herb-acupuncture was found to be effective to alleviate the arthritic symptoms in adjuvant-induced arthritic rats as regards the joint appearance and the expression profiles of inflammatory cytokines. In conclusion, therapeutic effects of HP herb-acupuncture on the rat with AIA might be related to anti-inflammatory activities of the hurb-acupuncture.

  2. Growth-promoting activity of Hominis Placenta extract on regenerating sciatic nerve

    Institute of Scientific and Technical Information of China (English)

    Tae-beom SEO; Dong-hee KIM; Seung-kiel PARK; Deok-chun YANG; Uk NAMGUNG; In-sun HAN; Jin-hwan YOON; In-chan SEOL; Yun-sik KIM; Hyun-kyung JO; Joung-jo AN; Kwon-eui HONG; Young-bae SEO

    2006-01-01

    Aim: Extract of Hominis Placenta (HP) has been used in oriental medicine as an agent for improving physiological function. The present study was conducted to investigate whether HP treatment in an experimental sciatic nerve injury animal model produces growth-promoting effects on regenerating peripheral nerve fibers after injury. Methods: After HP was injected into a sciatic nerve injury site, changes in protein levels were analyzed in the regenerating nerve area by Western blotting and immunofluorescence staining analyses. For quantitative assessment of axonal regeneration, a retrograde tracing technique was used to identify the neuronal cell bodies corresponding to regenerating axons, and the extent of neurite outgrowth in cultured dorsal root ganglia (DRG) sensory neurons prepared from animals that had experienced a sciatic nerve crush injury 7 d before neuron collection was analyzed. Results: Induction levels of axonal growth-associated protein (GAP-43) in the injured sciatic nerves were elevated by HP treatment. HP treatment also upregulated cell division cycle 2 (Cdc2) protein levels in the distal stump of the injured sciatic nerve. Induced Cdc2 protein was detected in Schwann cells, suggesting that Cdc2 kinase activity may be involved in the growth-promoting activity of regenerating axons via Schwann cell proliferation. Cell body measurement by retrograde tracing indicated that HP treatment produced significant increases in regenerating motor axons. Finally, HP treatment of cultured DRG sensory neurons significantly increased neurite arborization and elongation.Conclusion: HP promotes the regeneration of injured sciatic axons by upregulating the synthesis of regeneration-related protein factors such as GAP-43 and Cdc2.

  3. The associated microflora to the larvae of human bot fly Dermatobia hominis L. Jr. (Diptera: Cuterebridae and its furuncular lesions in cattle

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    E Sancho

    1996-06-01

    Full Text Available The microflora associated to furuncular lesions, larvae and pupae of Dermatobia hominis, as well as the relationships between parasite, host and microflora associated, as a comprehensive microsystem, has been studied. One hundred and two furuncular myiasis due to D. hominis larvae in several breeds of cattle were studied and the following bacterial species were significant: Staphylococcus aureus, S. epidermidis, S. warneri, Bacillus subtilis and Escherichia coli. Closely related, the microflora associated to 141 samples from first, second, third instar larva and both external surface and larval cavities has been studied. The representative associated microflora to the larvae were: S. aureus, B. subtilis, S. hycus and Moraxella phenylpiruvica, Moerella wisconsiensis, Proteus mirabilis and P. vulgaris, M. phenylpiruvica, M. wisconsiensis, P. mirabilis and P. rettgeri were the representative microflora associated to 64 pupae of D. hominis.

  4. Detection of T. vaginalis,M. hominis,M. genitalium, C. trachomatis, N. gonorrhoeae and U. urealyticum using Multiplex PCR

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    Tamara Brunelli

    2013-03-01

    Full Text Available Intoduction. The sexually transmitted diseases include a large group of infections affecting both the sexes. In this study we evaluated the prevalence of Trichomonas vaginalis, Mycoplasma hominis, Mycoplasma genitalium, Chlamydia trachomatis, Neisseria gonorrhoeae and Ureaplasma urealyticum in the Prato area during the period September 2010 – July 2011. Methods.We analysed different kind of samples (urine, endocervical swabs, urethral swabs, seminal fluids from hospitalized patients or referred to the Prato clinic subjects.The DNA was obtained using EZ1-DNA extraction kit and EZ1 instrument.The DNA was then amplified using the Seeplex STD6 kit (Seegene, Korea, identifying multiple pathogens simultaneously (T. vaginalis, M. hominis, M. genitalium, C. trachomatis, N. gonorrhoeae e U. urealyticum. The revelation was performed by electrophoresis on microchip (instrument Multina, Shimadzu, Japan. Results. 1136 samples from Italian and foreign patients were examined: 876 were endocervical swabs (77%, 103 urethral swabs (9%, 103 seminal fluids (9%, and 54 urines (5%. The number of females was higher than males [894 (78.7% vs 242 (21.3%]; the mean age of females was 37.0±11.6 years, whereas that of males was 41.5 ±12.63 years.The prevalence of urogenital pathogens was: 15 positive samples for T. vaginalis (1.3%, 56 for M. hominis (4.9%, 13 for M. genitalium (1.1%, 28 for C. trachomatis (2.5%, 8 for N. gonorrhoeae (0.7% and 87 for U. urealyticum (7.7%.Among all positive, 25 subjects were positive for more than one pathogen and in particular: one was positive for the presence of 4 pathogens, five presented 3 pathogens simultaneously and the remaining nineteen for 2 pathogens. Conclusions. This study provides data on the prevalence of sexually transmitted diseases in the hospital of Prato.

  5. Cryptosporidium hominis Is a Newly Recognized Pathogen in the Arctic Region of Nunavik, Canada: Molecular Characterization of an Outbreak.

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    Karine Thivierge

    2016-04-01

    Full Text Available Cryptosporidium is a leading cause of childhood diarrhea in low-resource settings, and has been repeatedly associated with impaired physical and cognitive development. In May 2013, an outbreak of diarrhea caused by Cryptosporidium hominis was identified in the Arctic region of Nunavik, Quebec. Human cryptosporidiosis transmission was previously unknown in this region, and very few previous studies have reported it elsewhere in the Arctic. We report clinical, molecular, and epidemiologic details of a multi-village Cryptosporidium outbreak in the Canadian Arctic.We investigated the occurrence of cryptosporidiosis using a descriptive study of cases with onset between April 2013 and April 2014. Cases were defined as Nunavik inhabitants of any age presenting with diarrhea of any duration, in whom Cryptosporidium oocysts were detected by stool microscopy in a specialised reference laboratory. Cryptosporidium was identified in stool from 51 of 283 individuals. The overall annual incidence rate (IR was 420 / 100,000 inhabitants. The IR was highest among children aged less than 5 years (1290 /100,000 persons. Genetic subtyping for stool specimens from 14/51 cases was determined by DNA sequence analysis of the 60 kDa glycoprotein (gp60 gene. Sequences aligned with C. hominis subtype Id in all cases. No common food or water source of infection was identified.In this first observed outbreak of human cryptosporidiosis in this Arctic region, the high IR seen is cause for concern about the possible long-term effects on growth and development of children in Inuit communities, who face myriad other challenges such as overcrowding and food-insecurity. The temporal and geographic distribution of cases, as well as the identification of C. hominis subtype Id, suggest anthroponotic rather than zoonotic transmission. Barriers to timely diagnosis delayed the recognition of human cryptosporidiosis in this remote setting.

  6. First report of furuncular myiasis caused by the larva of botfly, Dermatobia hominis, in a Taiwanese traveler.

    Science.gov (United States)

    Hu, Je-Ming; Wang, Chih-Chien; Chao, Li-Lian; Lee, Chung-Shinn; Shih, Chien-Ming

    2013-03-01

    A case of furuncular myiasis was reported for the first time in a 29-year-old young Taiwanese traveler returning from an ecotourism in Peru. Furuncle-like lesions were observed on the top of his head and he complained of crawling sensations within his scalp. The invasive larva of botfly, Dermatobia hominis, was extruded from the furuncular lesion of the patient. Awareness of cutaneous myiasis for clinicians should be considered for a patient who has a furuncular lesion and has recently returned from a botfly-endemic area.

  7. Dípteros fanídeos vetores de ovos de Dermatobia hominis em Campo Grande, Mato Grosso do Sul

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    Gomes Patrícia R.

    2002-01-01

    Full Text Available Determinou-se a importância epidemiológica de dípteros Fanniidae na infestação de mosca-do-berne, por meio da identificação das espécies presentes, da determinação daquelas utilizadas por Dermatobia hominis na veiculação de seus ovos, bem como, pelo conhecimento da dinâmica populacional das espécies mais abundantes. Foram utilizadas cinco armadilhas iscadas com fígado bovino cru deteriorado e colocadas em uma mata ciliar margeada por uma área de pastagem com presença constante de bovinos. O estudo foi desenvolvido em uma área da Embrapa Gado de Corte, em Campo Grande, Estado de Mato Grosso do Sul, Brasil, localizada a 20º27'S e 54º37'W. A captura dos insetos foi realizada semanalmente durante o período de 09/08/1999 a 03/08/2000. Foi capturado um total de 40.629 moscas da família Fanniidae, pertencendo a cinco espécies do gênero Fannia: F. pusio, F. heydenii, F. bahiensis e F. longipila, e uma a ser identificada. A espécie mais freqüente foi F. pusio, com 63,20% do total capturado, seguida de F. heydenii, com 28,82%. Somente 0,44% do total de fêmeas de F. heydenii (45 exemplares capturadas, principalmente nos meses de agosto e setembro, portavam ovos de D. hominis e o número médio, por indivíduo, foi de 15,98±7,13. Observaram-se ovos de D. hominis apenas na região abdominal dos vetores. F. heydenii predominou no período seco (maio a setembro e início do período chuvoso do ano (outubro e novembro. O número de exemplares portando ovos de D. hominis foi maior no final do período seco do ano, o que explica a alta incidência deste parasito em bovinos nos meses de setembro e outubro.

  8. Caracterização da variabilidade da mosca do berne Dermatobia hominis (Diptera : oestridae) atraves de dois marcadores moleculares

    OpenAIRE

    Taila Andrade Lemos

    2000-01-01

    Resumo: Dermatobia hominis (Linnaus Jr.) conhecida popularmente como mosca do berne. É uma das principais causadoras de miíase primária em vertebrados. sendo uma das principais pragas da pecuária nacional. causando grandes prejuízos à industria coureira. laticínios e frigoríficos. A mosca do berne apresenta uma biologia interessante e ciclo de vida bastante peculiar. o que dificuta a observação da espécie na natureza e a manutenção desta em laboratório. Por estes motivos. a caracterização da ...

  9. Dermabacter hominis: a usually daptomycin-resistant gram-positive organism infrequently isolated from human clinical samples

    OpenAIRE

    Fernández-Natal, I.; Sáez-Nieto, J A; Medina-Pascual, M J; Albersmeier, A.; Valdezate, S.; Guerra-Laso, J M; H. Rodríguez; Marrodán, T; Parras, T; TAUCH, A.; Soriano, F

    2014-01-01

    During a 12-year period, Dermabacter hominis was isolated from 21 clinical samples belonging to 14 patients attending a tertiary hospital in León, Spain. Samples included blood cultures (14), peritoneal dialysis catheter exit sites (three), cutaneous abscesses (two), an infected vascular catheter (one) and a wound swab (one). Identification was made by API Coryne™ V2.0, Biolog™ GP2 and 16S rRNA gene amplification. Six febrile patients had positive blood cultures (one, two or three sets) and a...

  10. PCR-Múltiple para el diagnóstico de Mycoplasma genitalium, Mycoplasma hominis, Ureaplasma parvum y Ureaplasma urealyticum

    Directory of Open Access Journals (Sweden)

    Nadia Rodríguez-Preval

    2007-04-01

    Full Text Available Mycoplasma genitalium, Mycoplasma hominis, Ureaplasma parvum y Ureaplasma urealyticum son especies relacionadas con enfermedades del tracto genitourinario, y particularmente con la uretritis no gonocócica (UNG en el hombre. Los cultivos de estos microorganismos resultan complicados, por lo que las técnicas moleculares, principalmente la reacción en cadena de la polimerasa (PCR, se han convertido en el principal método de detección de estos organismos. Objetivo: Implementar un método molecular basado en tecnología de genes para el diagnóstico de estas cuatro especies de micoplasmas genitales, aplicándolo en muestras clínicas de pacientes con UNG. Material y métodos: Se crearon las condiciones para un PCR-Múltiple para identificar estas especies empleando como muestra ADN de referencia, utilizando los juegos de cebadores complementarios a fragmentos de los genes de la proteína adhesiva de M. genitalium (MgPa, ARN ribosomal 16S de M. hominis, región espaciadora entre los genes del ARN ribosomal 16S y 23S de U. parvum, y de la región espaciadora adyacente al gen de la ureasa y específico para U. urealyticum, siendo un método específico y sensible. Resultados: Al analizar 34 muestras de exudado uretral, 27 correspondieron a la clase Mollicutes, obteniéndose 14,8% de positividad a M. genitalium, 18,5% a M. hominis, 11,1% a U. urealyticum y 3,7%. a U. parvum. Con este trabajo se realizó por primera vez el diagnóstico de M. genitalium, M. hominis, U. parvum y U. urealyticum en muestras uretrales de pacientes cubanos. Conclusión: Se recomienda incluir el diagnóstico de estas especies en un mayor número de pacientes cubanos con síntomas uretrales, para validar el método propuesto y conocer la relación de estos microorganismos con la UNG.

  11. Prevalence and antibiotic susceptibility of Mycoplasma hominis and Ureaplasma urealyticum in genital samples collected over 6 years at a Serbian university hospital

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    Dusan Skiljevic

    2016-01-01

    Full Text Available Background: Mycoplasma hominis and Ureaplasma urealyticum are implicated in a wide array of infectious diseases in adults and children. Since some species have innate or acquired resistance to certain types of antibiotics, antibiotic susceptibility testing of mycoplasma isolated from the urogenital tract assumes increasing importance. Aims: To evaluate the prevalence and antibiotic susceptibility of M. hominis and U. urealyticum in genital samples collected between 2007 and 2012. Methods: Three hundred and seventy three patients presenting with symptoms of sexually transmitted diseases, infertility or risky sexual behaviour, who had not taken antibiotics in the previous 6 weeks and had ≥10 WBC per high power field on genital smears were studied. Urethral samples were taken in men and endocervical samples in women. The mycoplasma IST-2 kit was used for organism identification and for testing susceptibility to doxycycline, josamycin, ofloxacin, erythromycin, tetracycline, ciprofloxacin, azithromycin, clarithromycin and pristinamycin. Results: U. urealyticum was isolated from 42 patients and M. hominis from 11 patients. From 9.8% of isolates, both organisms were grown. All M. hominis isolates were resistant to tetracycline, clarithromycin and erythromycin while U. urealyticum was highly resistant to clarithromycin (94.6%, tetracycline (86.5%, ciprofloxacin (83.8% and erythromycin (83.8%. M. hominis was sensitive to doxycycline (83.3% and ofloxacin (66.7% while most U. urealyticum strains were sensitive to doxycycline (94.6%. Limitations: Inability of the commercial kit used in the study to detect other potentially pathogenic urogenital mycoplasmas (Ureaplasma parvum, Mycoplasma genitalium. Conclusion: There is significant resistance of U. urealyticum and M. hominis to tetracycline and macrolides. The most active tetracycline for genital mycoplasmas was found to be doxycycline, which continues to be the drug of first choice.

  12. Pleiotropic effects of Blastocystis spp. Subtypes 4 and 7 on ligand-specific toll-like receptor signaling and NF-κB activation in a human monocyte cell line.

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    Joshua D W Teo

    Full Text Available Blastocystis spp. is a common enteric stramenopile parasite that colonizes the colon of hosts of a diverse array of species, including humans. It has been shown to compromise intestinal epithelial cell barrier integrity and mediate the production of pro-inflammatory cytokines and chemokines. Mucosal epithelial surfaces, including the intestinal epithelium, are increasingly recognized to perform a vital surveillance role in the context of innate immunity, through the expression of pathogen recognition receptors, such as Toll-like receptors (TLRs. In this study, we use the human TLR reporter monocytic cell line, THP1-Blue, which expresses all human TLRs, to investigate effects of Blastocystis on TLR activation, more specifically the activation of TLR-2, -4 and -5. We have observed that live Blastocystis spp. parasites and whole cell lysate (WCL alone do not activate TLRs in THP1-Blue. Live ST4-WR1 parasites inhibited LPS-mediated NF-κB activation in THP1-Blue. In contrast, ST7-B WCL and ST4-WR1 WCL induced pleiotropic modulation of ligand-specific TLR-2 and TLR-4 activation, with no significant effects on flagellin-mediated TLR-5 activation. Real time-qPCR analysis on SEAP reporter gene confirmed the augmenting effect of ST7-B on LPS-mediated NF-κB activation in THP1-Blue. Taken together, this is the first study to characterize interactions between Blastocystis spp. and host TLR activation using an in vitro reporter model.

  13. High Frequency of Latent Conjunctival C. trachomatis, M. hominis, and U. urealyticum Infections in Young Adults with Dry Eye Disease

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    Ernest V. Boiko

    2014-01-01

    Full Text Available Aim. To determine the frequency of detection of conjunctival C. trachomatis (CT, M. hominis (MH, and U. urealyticum (UU infections in young adults with dry eye disease (DED, since these infections may potentially produce the chronic subclinical inflammation characteristic of DED. Materials and Methods. The study included subjects of 25–45 years of age, divided into the DED (n=114 and nondry eye control (n=98 groups, with the diagnosis based on self-reported complaints, biomicroscopy, the Schirmer I test, and break-up time. All patients had conjunctival scrapings taken to detect CT, MH, and UU with direct fluorescent-antibody assay kits. Results. At least one of the three microorganisms was found in 87.7% of the DED patients versus 8.2% of the controls. Of all the DED patients, 63.2%, 50.8%, and 42.1% were found to be infected with CT, MH, and UU, respectively. Multiple pathogens were identified in 65% of the DED patients found to be infected. CT infection was detected in 6.1% of the controls. Conclusion. C. trachomatis, M. hominis, and U. urealyticum were detected with high frequency in the conjunctiva of young adults with DED and may be an important risk factor for DED in them.

  14. High Frequency of Latent Conjunctival C. trachomatis, M. hominis, and U. urealyticum Infections in Young Adults with Dry Eye Disease

    Science.gov (United States)

    Boiko, Ernest V.; Pozniak, Alexei L.; Maltsev, Dmitrii S.; Suetov, Alexei A.; Nuralova, Irina V.

    2014-01-01

    Aim. To determine the frequency of detection of conjunctival C. trachomatis (CT), M. hominis (MH), and U. urealyticum (UU) infections in young adults with dry eye disease (DED), since these infections may potentially produce the chronic subclinical inflammation characteristic of DED. Materials and Methods. The study included subjects of 25–45 years of age, divided into the DED (n = 114) and nondry eye control (n = 98) groups, with the diagnosis based on self-reported complaints, biomicroscopy, the Schirmer I test, and break-up time. All patients had conjunctival scrapings taken to detect CT, MH, and UU with direct fluorescent-antibody assay kits. Results. At least one of the three microorganisms was found in 87.7% of the DED patients versus 8.2% of the controls. Of all the DED patients, 63.2%, 50.8%, and 42.1% were found to be infected with CT, MH, and UU, respectively. Multiple pathogens were identified in 65% of the DED patients found to be infected. CT infection was detected in 6.1% of the controls. Conclusion. C. trachomatis, M. hominis, and U. urealyticum were detected with high frequency in the conjunctiva of young adults with DED and may be an important risk factor for DED in them. PMID:24967096

  15. Treatment for intractable anemia with the traditional Chinese medicines Hominis Placenta and Cervi Cornus Colla (deer antler glue

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    Yasuyo Hijikata

    2009-05-01

    Full Text Available Yasuyo Hijikata1, Takashi Kano2, Lu Xi31Toyodo Hijikata Clinic, Osaka, Japan; 2Kano Clinic, Osaka city, Osaka, Japan; 3Traditional Chinese Medicine Institute, Si-chuan Province, ChinaObjective: Intractable anemia, such as aplastic anemia or that presumably associated with chronic herpes virus infections, sometimes require bone marrow transplant. We investigated the use of traditional Chinese medicine (TCM for the treatment of intractable anemia. Method: Placenta Hominis (PH, steam boiled and roasted, and Cervi Cornus Colla (deer antler glue has been used in China for hundreds of years to treat anemia. After consent was obtained, we prescribed these two materials for a 74-year-old female with aplastic anemia and a 26-year-old male with presumably a virus-induced anemia. Concomitant conventional therapy was continued in both patients as prescribed by their respective attending physicians. Conclusion: Conventional therapy with steroid hormones, immunosuppressive drugs, platelet and erythrocyte transfusions were not effective in these patients. In addition, both patients suffered from serious side effects. In two patients, ingestion of Placenta Hominis and Cervi Cornus Colla with TCM prescriptions increased the platelet and enhanced the hemoglobin concentration in several months of therapy accompanied by a dramatic improvement in quality of life. The addition to conventional therapy of PH and Cervi Cornus Colla, the latter of which is very easy to obtain, may be one of the potentially advantageous choices in case of otherwise intractable anemia.Keywords: placenta, antler glue, Cervi Cornus Colla, anemia, aplastic anemia

  16. Structural studies provide clues for analog design of specific inhibitors of Cryptosporidium hominis thymidylate synthase-dihydrofolate reductase.

    Science.gov (United States)

    Kumar, Vidya P; Cisneros, Jose A; Frey, Kathleen M; Castellanos-Gonzalez, Alejandro; Wang, Yiqiang; Gangjee, Aleem; White, A Clinton; Jorgensen, William L; Anderson, Karen S

    2014-09-01

    Cryptosporidium is the causative agent of a gastrointestinal disease, cryptosporidiosis, which is often fatal in immunocompromised individuals and children. Thymidylate synthase (TS) and dihydrofolate reductase (DHFR) are essential enzymes in the folate biosynthesis pathway and are well established as drug targets in cancer, bacterial infections, and malaria. Cryptosporidium hominis has a bifunctional thymidylate synthase and dihydrofolate reductase enzyme, compared to separate enzymes in the host. We evaluated lead compound 1 from a novel series of antifolates, 2-amino-4-oxo-5-substituted pyrrolo[2,3-d]pyrimidines as an inhibitor of Cryptosporidium hominis thymidylate synthase with selectivity over the human enzyme. Complementing the enzyme inhibition compound 1 also has anti-cryptosporidial activity in cell culture. A crystal structure with compound 1 bound to the TS active site is discussed in terms of several van der Waals, hydrophobic and hydrogen bond interactions with the protein residues and the substrate analog 5-fluorodeoxyuridine monophosphate (TS), cofactor NADPH and inhibitor methotrexate (DHFR). Another crystal structure in complex with compound 1 bound in both the TS and DHFR active sites is also reported here. The crystal structures provide clues for analog design and for the design of ChTS-DHFR specific inhibitors.

  17. Epidemiologic and clinical characteristics of pelvic inflammatory disease associated with Mycoplasma hominis, Chlamydia trachomatis, and Neisseria gonorrhoeae.

    Science.gov (United States)

    Miettinen, A; Saikku, P; Jansson, E; Paavonen, J

    1986-01-01

    We studied selected epidemiologic, clinical, serologic, and microbiologic findings and their interrelationships among 57 women with acute pelvic inflammatory disease (PID). Cervical cultures positive for Neisseria gonorrhoeae alone and for both N. gonorrhoeae and Chlamydia trachomatis were associated with young age, nulliparity, and use of birth-control pills. Positive serologic findings for C. trachomatis were associated with the isolation of C. trachomatis and/or N. gonorrhoeae from the cervix and predicted the presence of a pelvic mass. High levels of antibody to Mycoplasma hominis were associated with increasing age and parity, and predicted a low concentration of C-reactive protein (CRP), a long hospital stay, and a high convalescent-phase erythrocyte sedimentation rate (ESR). Women with recurrent PID had higher titers of antibody to C. trachomatis than those with primary PID. The use of an intrauterine contraceptive device predicted high CRP, high acute-phase ESR, long hospital stay, and was frequently associated with positive serologic tests for M. hominis. These results demonstrate that the clinical picture of PID depends not only on the microorganisms involved but also on many epidemiologic factors such as age, contraceptive method, and parity.

  18. DNA sequencing reveals limited heterogeneity in the 16S rRNA gene from the rrnB operon among five Mycoplasma hominis isolates

    DEFF Research Database (Denmark)

    Mygind, T; Birkelund, Svend; Christiansen, Gunna

    1998-01-01

    To investigate the intraspecies heterogeneity within the 16S rRNA gene of Mycoplasma hominis, five isolates with diverse antigenic profiles, variable/identical P120 hypervariable domains, and different 16S rRNA gene RFLP patterns were analysed. The 16S rRNA gene from the rrnB operon was amplified...

  19. Mycoplasma hominis infection of Trichomonas vaginalis is not associated with metronidazole-resistant trichomoniasis in clinical isolates from the United States.

    Science.gov (United States)

    Butler, Sara E; Augostini, Peter; Secor, W Evan

    2010-09-01

    Trichomonas vaginalis is a protozoan parasite that is the cause of the most common non-viral sexually transmitted disease, trichomoniasis. Metronidazole and tinidazole are the only drugs approved for treatment of T. vaginalis infections in the USA. However, drug resistance exists and some patients are allergic to these medications. Furthermore, the exact mechanism of metronidazole resistance remains undefined and current testing methods require several weeks before results are available. Identification of the mechanism of drug resistance may lead to the development of molecular tools to detect drug resistance, and quicker results for clinical treatment. In a recent study, Chinese T. vaginalis isolates that were polymerase chain reaction (PCR) positive for Mycoplasma hominis DNA demonstrated greater in vitro resistance to metronidazole than isolates with no evidence of M. hominis infection. To evaluate this finding in isolates from a distinct epidemiologic setting, we tested 55 T. vaginalis isolates collected from patients in the USA through the Centers for Disease Control and Prevention metronidazole susceptibility testing service. One half of the isolates demonstrated resistance to metronidazole by an in vitro sensitivity assay. Of the metronidazole-resistant T. vaginalis isolates, 18% were PCR positive for M. hominis, as were 22% of the metronidazole-susceptible T. vaginalis isolates (p = 0.746). We also observed no change in metronidazole sensitivity of two infected T. vaginalis isolates after they were cleared of their M. hominis infection by culturing the isolates in antibiotics. Thus, M. hominis infection of USA T. vaginalis isolates did not correlate with in vitro resistance to metronidazole.

  20. 论“情理共同体”%Development as Civitas Homini

    Institute of Scientific and Technical Information of China (English)

    林曦

    2016-01-01

    Rapid development in a transitioning society will surely bring to fore as many positive consequences as negative ones. Drawing upon Amartya Sen and Fromm, this concept of Entwicklungsdialektik is put forward to generalize the pathological representation of this highly mixed record of development.The solution to such Entwicklungsparadox lies in the shift from mechanical solidarity (as a characteristic of traditional society)to organic solidarity (typical of a modern one),enabling the latter to increase in both scale and depth in the society.A critical reflection on the Durkheimian thesis on “occupational groups/corporation”paves the way for us to propose the new concept of civitas homini ,which is characterized by “the new quintochomy of human relations,”“an all-in-all consideration of both Vernünftigkeit and Rationalität,” “priority to individual choice preferences,” and “intersubjective recognition.” This concept is of greater hermeneutic value and empirical validity for the reality in contemporary China.%对于一个转型社会而言,高速的发展必然会带来同样多的积极和消极结果。在综合阿玛蒂亚·森和弗洛姆论说的基础上,笔者提出了“发展辩证法”的概念,来总括这种由发展而导致的“喜忧参半”的社会病理学状况。解决这种“发展悖论”的方式在于从传统社会的“机械团结”过渡到现代社会的“有机团结”,促进“有机团结”在转型社会中的扩大和增加。笔者在批判吸收迪尔凯姆“职业团体”论说的基础上提出了“情理共同体”这一概念,指出了其所包含的“新五伦说”、“情理兼顾”、“个体选择优先”、“主体间性承认”等四个基本特征,并认为这样一种“非理想”的理论概念能够更有效地应用于当下中国转型社会的现实。

  1. Is "dried stool spots on filter paper method (DSSFP)" more sensitive and effective for detecting Blastocystis spp. and their subtypes by PCR and sequencing?

    Science.gov (United States)

    Seyer, Ayse; Karasartova, Djursun; Ruh, Emrah; Güreser, Ayse Semra; Imir, Turgut; Taylan-Ozkan, Aysegul

    2016-12-01

    PCR and DNA sequencing are currently the diagnostic methods of choice for detection of Blastocystis spp. and their suptypes. Fresh or frozen stool samples have disadvantages in terms of several aspects such as transportation, storage, and existence of PCR inhibitors. Filter paper technology may provide a solution to these issues. The aim of the present study was to detect Blastocystis spp. and their subtypes by employing two different preservation methods: conventional frozen stool (FS) and dried stool spots on filter paper (DSSFP). Concentration and purity of DNA, sensitivity of PCR, and DNA sequencing results obtained from the two methods were also compared. A total of 230 fecal samples were included and separated into two parts: one part of the fecal samples were directly frozen and stored at -20 °C. The remaining portion of the specimens were homogenized with saline and spread onto the filter papers as thin layer with a diameter of approximately 3 cm. After air-dried, the filter papers were stored at room temperature. DSSFP samples were collected by scraping from the filter papers. DNA were extracted by EURx Stool DNA Extraction Kit from both samples. Concentration and purity were measured with Nano-Drop, then PCR and sequencing were conducted for detection of Blastocystis spp. and its genotypes. Pure DNA was obtained with a A260/A280 ratio of 1.7-2.2 in both methods. DNA yield from FS was 25-405 ng/μl and average DNA concentration was 151 ng/μl, while these were 7-339 and 122 ng/μl for DSSFP, respectively. No PCR inhibition was observed in two methods. DNA from DSSFP were found to be stable and PCR were reproducible for at least 1 year. FS-PCR- and DSSFP-PCR-positive samples were 49 (21.3 %) and 58 (25.3 %), respectively (p = 0.078). The 43 specimens were concordantly positive by both FS-PCR and DSSFP-PCR. When the microscopy was taken as the gold standard, sensitivity of DSSFP-PCR and FS-PCR was 95.5 and 86.4 %, while specificity of both

  2. Genomic Variation in IbA10G2 and Other Patient-Derived Cryptosporidium hominis Subtypes.

    Science.gov (United States)

    Sikora, Per; Andersson, Sofia; Winiecka-Krusnell, Jadwiga; Hallström, Björn; Alsmark, Cecilia; Troell, Karin; Beser, Jessica; Arrighi, Romanico B G

    2017-03-01

    In order to improve genotyping and epidemiological analysis of Cryptosporidium spp., genomic data need to be generated directly from a broad range of clinical specimens. Utilizing a robust method that we developed for the purification and generation of amplified target DNA, we present its application for the successful isolation and whole-genome sequencing of 14 different Cryptosporidium hominis patient specimens. Six isolates of subtype IbA10G2 were analyzed together with a single representative each of 8 other subtypes: IaA20R3, IaA23R3, IbA9G3, IbA13G3, IdA14, IeA11G3T3, IfA12G1, and IkA18G1. Parasite burden was measured over a range of more than 2 orders of magnitude for all samples, while the genomes were sequenced to mean depths of between 17× and 490× coverage. Sequence homology-based functional annotation identified several genes of interest, including the gene encoding Cryptosporidium oocyst wall protein 9 (COWP9), which presented a predicted loss-of-function mutation in all the sequence subtypes, except for that seen with IbA10G2, which has a sequence identical to the Cryptosporidium parvum reference Iowa II sequence. Furthermore, phylogenetic analysis showed that all the IbA10G2 genomes form a monophyletic clade in the C. hominis tree as expected and yet display some heterogeneity within the IbA10G2 subtype. The current report validates the aforementioned method for isolating and sequencing Cryptosporidium directly from clinical stool samples. In addition, the analysis demonstrates the potential in mining data generated from sequencing multiple whole genomes of Cryptosporidium from human fecal samples, while alluding to the potential for a higher degree of genotyping within Cryptosporidium epidemiology.

  3. Effect of Mycoplasma hominis and cytomegalovirus infection on pregnancy outcome: A prospective study of 200 Mongolian women and their newborns

    Science.gov (United States)

    Batbaatar, Gunchin; Tsogtsaikhan, Sandag; Enkhtsetseg, Jamsranjav; Enkhjargal, Altangerel; Pfeffer, Klaus; Adams, Ortwin; Battogtokh, Chimeddorj

    2017-01-01

    In Mongolia, diagnostic tests for the detection of the sexually transmitted mycoplasmas, ureaplasmas, Herpes simplex virus (HSV), and cytomegalovirus (CMV) are currently not routinely used in clinical settings and the frequency of these STIs are enigmatic. The prevalence of these STI pathogens were prospectively evaluated among 200 Mongolian pregnant women and their newborns and correlated with pregnancy outcome. TaqMan PCRs were used to detect bacterial and viral STI pathogens in pre-birth vaginal swabs of the pregnant women and in oral swabs of their newborns. A standardized questionnaire concerning former and present pregnancies was developed and linear regression analysis was used to correlate pathogen detection with pregnancy outcome. Ureaplasmas were the most prevalent of the tested pathogens (positive in 90.5% positive women and 47.5% newborns), followed by mycoplasmas (32.5% and 7.5%), chlamydia (14.5% and 7.5%), trichomonas (8.5% and 4.0%) and gonococcus (0.5% and 0%). CMV was found in 46.5% of the pregnant women and in 10.5% of their newborns, whereas HSV-2 was detected in only two mothers. Multiple regression analyses indicate that colonization of the mothers with U. urealyticum, M. hominis, T. vaginalis or CMV is associated with transmission to newborns and that transmission of M. hominis or CMV from Mongolian pregnant women to offspring is associated with reduced neonatal length and gestational age. Thus, diagnostic tests for their detection should be implemented in the clinical settings in Mongolia. PMID:28257513

  4. Morphology of human Fallopian tubes after infection with Mycoplasma genitalium and Mycoplasma hominis - in vitro organ culture study

    DEFF Research Database (Denmark)

    Baczynska, A.; Funch, Peter; Fedder, J.;

    2007-01-01

    that mycoplasmas can cause tubal factor infertility. We analysed the effects of infections with Mycoplasma hominis and Mycoplasma genitalium on the HFT epithelium and compared them with the effects of infections with genital pathogens: Chlamydia trachomatis and Neisseria gonorrhoeae. METHODS: We used an in vitro...... on the HFT was, however, very moderate when compared with the extensive damage of the epithelium caused by N. gonorrhoeae or C. trachomatis....

  5. Effects of Hominis Placenta Aqua-acupuncture on Kidney and Liver Intoxicated by HgCI2 in Rats

    Directory of Open Access Journals (Sweden)

    Lee, Sang-Keel

    2000-12-01

    Full Text Available Objective: This study was perfomled to examine the therapeutic effect of aqua-acupuncture solution of Hominis Placenta(HP on kidney and liver intoxicated by HgCl2 in rats. Methods: 10% and 25% HP aqua-acupuncture were carried out everyday for 8 days on corresponding bilateral loci of Shinsu(BL23 and Kansu(BL18, respectively, after mercuric chloride intoxication in rats. Thereafter BUN, creatinine, GOT, GPT, ALP, -GT, albumin and total bilirubin were measured before intoxication, and at the 4th and the 8th experimental day. Histopathological and immunochemical observation were also carried out. Results: 1. It showed significant decreases of BUN in the group of 10% HP aqua-acupuncture into Shinsu on the 4th experimental day as compared with the control group. 2. It showed significant decreases of creatinine in the group of 10% HP aqua-acupuncture into Shinsu on the 4th and the 8th experimental days as compared with the control group. 3. There were not any significant changes of GOT, GPT, ALP, γ-GT, albumin and total bilirubin in the HP aqua-acupuncture groups compared with the control group. 4. By the histopathological observations on kidney under a light microscope, all the 10% and 25% HP aqua-acupuncture into Shinsu showed the preventive effect on tubulo-interstitial necrosis and muItifocal calcification in tubular lumen respectively compared with the control group. 5. By the histopathological observations on liver under a light mIcroscope, the groups 10% and 25% HP aqua-acupuncture into Kansu did not show any significant changes in the liver compared with the control group. 6. By the immunochemical analysis of heat shock protein(hsp and glucose-regulated protein(grp in rat renal cortex, the expressions of hsp70 and grp78 were decreased in the and HP aqua-acupuncture into Shinsu respectively compared with the control group. Conclusion: These results suggest that Hominis Placenta aqua-acupuncture have an effect on prevention and protection of

  6. Detection of Cryptosporidium hominis and novel Cryptosporidium bat genotypes in wild and captive Pteropus hosts in Australia.

    Science.gov (United States)

    Schiller, Sabine Eva; Webster, Koa Narelle; Power, Michelle

    2016-10-01

    Spillover of zoonotic pathogens from wildlife to humans has been identified as a primary threat to global health. In contrast, the process of reverse pathogen transmission (zooanthroponosis), whereby pathogens move from humans into wildlife species is still largely unexplored. Globally, increasing urbanisation and habitat loss are driving many wildlife species into urban and regional centres. In Australia, large numbers of flying foxes now live in close proximity to humans, increasing the risk of zooanthroponosis. The protozoan parasite Cryptosporidium is an emerging zoonotic parasite that infects a wide range of vertebrates yet there are limited studies on transmission potential of Cryptosporidium between humans and urban wildlife. To explore the presence of zooanthroponosis in flying foxes in Australia the occurrence and diversity of Cryptosporidium was investigated in urbanised wild and captive flying foxes. PCR screening of faecal samples (n=281) from seven wild sites and two captive facilities identified the presence of Cryptosporidium in 3.2% (95% CI 1.5% to 6.0%) of faecal samples. In faecal samples from wild sites Cryptosporidium occurrence was 1.7% (95% CI 0.3% to 4.8%) versus 5.9% (95% CI 2.2% to 12.4%) in samples from captive individuals, with no significant difference between captive and wild sites (p=0.077). Multilocus sequencing using three loci, 18s rDNA, actin and gp60 was used to identify Cryptosporidium in flying fox species. The host specific Cryptosporidium hominis was identified in faecal samples from two captive flying foxes, and one of these samples was confirmed as C. hominis at both actin and gp60. Sequencing of the 18s rDNA also revealed four novel Cryptosporidium genotypes in wild and captive individuals, actin and gp60 amplification and sequencing were unreliable for all four novel genotypes. These novel genotypes have been designated Cryptosporidium bat genotypes VIII-XI. This first report of Cryptosporidium spp. in Australian flying

  7. Desenvolvimento pós-embrionário do intestino médio de Dermatobia hominis (Linnaeus Jr. (Diptera, Cuterebridae Post-embryonic development of Dermatobia hominis (Linnaeus Jr. (Diptera, Cuterebridae midgut

    Directory of Open Access Journals (Sweden)

    Edy de Lello

    2001-03-01

    Full Text Available Dermatobia hominis (Linnaeus, 1781 midgut is internally lined by an epithelium of polytenic cells, some low others prismatic with well developed brush border. Their apical portion are enlarged by secretory vesicles, forming button-like structures that are pinched off to the lumen, some accompained by the nucleus characterizing apocrine and holocrine secretions. This epithelium is gradually renewed by small, non polytenic regenerative cells, found scattered at its basal portion. At the end of the third instar the metamorphosis begins. The epithelial cells present signs of degeneration and at the first day of pupation the regenerative cells increase in number. By the 5th day of pupation these regenerative cells, besides being increased in number, differentiate themselves into two layers: one similar to the dense conective tissue that sustainning the larval epithelium is pinched off to the midgut lumen forming the "yellow bodies"; the other, develops right under it as the imaginal epitelium. The disorganized muscles bundles of the midgut wall, are invaded by phagocytes. At the end of pupation the midgut has a low prismatic epithelium with brush-border. In the adult, the torax portion of the midgut has prismatic homogeneously basophilic epithelium while in the abdominal portion the epithelium is made of high prismatic cells full of small vacuoles. The larval midgut epithelium suffers programmed cell death non compatible with apoptose. During the metamorphosis the midgut lenght diminishes from 31mm in the larva to 14mm in the adult.

  8. Ultrastructure of the ovary of Dermatobia hominis (Diptera: cuterebridae. I. Development during the 3rd larval instar

    Directory of Open Access Journals (Sweden)

    E. A. Gregorio

    1990-09-01

    Full Text Available The ultrastructure and distribution of gonial and somatic cells in the ovary of Dermatobia hominis was studied during the 3rd larval instar. In larvae weighing between 400 and 500 mg, the ovary is partially divided into basal and apical regions by oblong somatic cells that penetrate from the periphery; these cells show ovoid nucleus and cytoplasm full of microtubules. In both regions, gonial cells with regular outlines, large nucleus and low electron-density cytoplasm are scattered among the interstitial somatic cells. These later cells have small nucleus and electrodense cytoplasm. Clear somatic cells with small nucleus and cytoplasm of very low electron-density are restrict to the apical region of the gonad. Degenerating interstitial somatic cells are seen in the basal portion close to the ovary peduncle. During all this larval period the morphological features of the ovary remain almost the same. At the end of the period there is a gradual deposition of glycogen in the cytoplasm of the somatic cells, increase in the number and density of their mitochondria plus nuclear modification as membrane wrinkling and chromatin condensation in masses.

  9. Improved method for expression and isolation of the Mycoplasma hominis arginine deiminase from the recombinant strain of Escherichia coli.

    Science.gov (United States)

    Fayura, Lyubov R; Boretsky, Yuriy R; Pynyaha, Yuriy V; Wheatley, Denys N; Sibirny, Andriy A

    2013-09-20

    Arginine deiminase is a promising anticancer drug active against melanoma, hepatocarcinoma and other tumors. Recombinant strains of Escherichia coli that express arginine deiminase from pathogenic bacteria Mycoplasma have been developed. However, production costs of heterologous arginine deiminase are high due to use of an expensive inducer and extraction buffer, as well as using diluted culture for enzyme induction. We report on a new advanced protocol for Mycoplasma hominis arginine deiminase expression, extraction and renaturation. The main improvements include manipulation with dense suspensions of E. coli, use of lactose instead of isopropyl β-D-1-thiogalactopyranoside as an inducer and a cheaper but not less efficient buffer for solubilization of arginine deiminase inclusion bodies. In addition, supplementation of the storage culture medium with glucose and substrate (arginine) significantly stabilized the recombinant arginine deiminase producer. Homogenous preparations of recombinant arginine deiminase were obtained using anion-exchange and hydrophobic chromatography. The purified enzyme retained a specific activity of 30-34 U/mg for 12 months when stored at 4°C in 20 mM sodium phosphate buffer pH 7.2 containing 1 M NaCl.

  10. PCR Research of Mycoplasma hominis in Trichomonas vaginalis Cells of Sichuan Province%四川地区阴道毛滴虫内人型支原体的PCR检测

    Institute of Scientific and Technical Information of China (English)

    朱晓燕; 王雅静; 毕世樑; 张仁刚

    2009-01-01

    20 isolates of Trichomonas vaginalis were collected from clinical patients in hospital. After pure cultivation, genomic DNA of T. vaginalis was extracted. A pair of specific 16S rDNA primers was designed based on the sequence of Mycoplasma hominis. M. hominis in T. vaginalis cells were detected by PCR. Altogether 20 isolates of T. vaginalis were collected, 13 out of 20 T. vaginalis isolates harboured M. hominis, and the symbiosis ratio between T. vaginalis and M. hominis was up to 65%. It suggested that the symbiotic relationship between T. vaginalis and M. hominis occurred commonly in Sichuan, China.%从临床上分离获得20株阴道毛滴虫虫株,经纯化培养后,提取基因组DNA.以人型支原体16S rDNA序列设计特异性引物,利用PCR技术检测阴道毛滴虫内的人型支原体,结果有13株为人型支原体阳性,感染率为65%,表明阴道毛滴虫与人型支原体的共生关系在中国四川具有普遍性.

  11. Detección de parásitos intestinales en agua y alimentos de Trujillo, Perú

    Directory of Open Access Journals (Sweden)

    Gregorio Pérez-Cordón

    2008-01-01

    Full Text Available Detectamos distintas especies de parásitos intestinales, tanto protozoos como helmintos, presentes en muestras de agua provenientes de acequias y pozos (Giardia lamblia, Blastocystis hominis, Entamoeba coli, Cyclospora cayetanensis, Cryptosporidium spp. y Balantidium coli, así como en alimentos crudos y cocidos (Giardia lamblia, Cyclospora cayetanensis., Endolimax nana, Iodamoeba butschlii y Blastocystis hominis Fasciola hepatica y Ascaris lumbricoides recolectadas en varios distritos de la provincia de Trujillo, Perú.

  12. Multicenter Evaluation of BD Max Enteric Parasite Real-Time PCR Assay for Detection of Giardia duodenalis, Cryptosporidium hominis, Cryptosporidium parvum, and Entamoeba histolytica

    Science.gov (United States)

    Relich, R. F.; Doyle, L.; Espina, N.; Fuller, D.; Karchmer, T.; Lainesse, A.; Mortensen, J. E.; Pancholi, P.; Veros, W.; Harrington, S. M.

    2016-01-01

    Common causes of chronic diarrhea among travelers worldwide include protozoan parasites. The majority of parasitic infections are caused by Giardia duodenalis, Entamoeba histolytica, Cryptosporidium parvum, and Cryptosporidium hominis. Similarly, these species cause the majority of parasitic diarrhea acquired in the United States. Detection of parasites by gold standard microscopic methods is time-consuming and requires considerable expertise; enzyme immunoassays and direct fluorescent-antibody (DFA) stains have lowered hands-on time for testing, but improvements in sensitivity and technical time may be possible with a PCR assay. We performed a clinical evaluation of a multiplex PCR panel, the enteric parasite panel (EPP), for the detection of these common parasites using the BD Max instrument, which performs automated extraction and amplification. A total of 2,495 compliant specimens were enrolled, including 2,104 (84%) specimens collected prospectively and 391 (16%) specimens collected retrospectively. Approximately equal numbers were received in 10% formalin (1,273 specimens) and unpreserved (1,222 specimens). The results from the EPP were compared to those from alternate PCR and bidirectional sequencing (APCR), as well as DFA (G. duodenalis and C. parvum or C. hominis) or trichrome stain (E. histolytica). The sensitivity and specificity for prospective and retrospective specimens combined were 98.2% and 99.5% for G. duodenalis, 95.5% and 99.6 for C. parvum or C. hominis, and 100% and 100% for E. histolytica, respectively. The performance of the FDA-approved BD Max EPP compared well to the reference methods and may be an appropriate substitute for microscopic examination or immunoassays. PMID:27535690

  13. Draft Genome Sequences of Microbacterium hominis LCDC-84-0209T Isolated from a Human Lung Aspirate and Microbacterium laevaniformans LCDC 91-0039 Isolated from a Human Blood Culture.

    Science.gov (United States)

    Bernier, Anne-Marie; Bernard, Kathryn

    2016-01-01

    Draft genomes for Microbacterium hominis 84-0209(T) and M. laevaniformans 91-0039 were studied. Genome sizes (bps, [G+C contents]) were 3,506,522 (70.96%) and 2,999,965 (69.51%), respectively. Annotation revealed: (M. hominis) three rRNA sequences, 45 tRNA genes, and 3,218 coding sequences; (M. laevaniformans) three rRNA sequences, 49 tRNA genes, and 2,874 coding sequences.

  14. A Study on Diagnosis of Mycoplasma Hominis in The Urogenital Tract By Nested Polymerase Chain Reaction with First Void Urine in Men

    Institute of Scientific and Technical Information of China (English)

    徐斌; 温泉; 刘洋; 张林

    2001-01-01

    Objectives: To evaluate the efficacy of nested polymerase chain reaction (PCR) with first void urine (FVU) for the diagnosis of Mycoplasma hominis in male patients.Methods: Matched FVU specimens and urethral swabs were collected from 194 male patients with Nongonococcal Urethritis and tested by nested PCR and cell culture. Cell culture was used as a gold standard for evaluating other assay techniques.Results: For FVU nested PCR assay and FVU cell culture,our results showed that the sensitivity was 100% and 93.3%;specificity was 97.0% and 98.2%; positive predictive value (PPV) was 85.7% and 90.3%, negative predictive value (NPV)was 100% and 98.8%, respectively. The total consistency between the two techniques was 97.4 %.Conclusions: For the diagnosis of Mycoplasma hominis in men, nested PCR detecting FVU is a highly sensitive and specific method. First void urine can replace swab culture or PCR in terms of acceptability and feasibility.

  15. Ocorrência de vetores biológicos da Dermatobia hominis (L.Jr., 1781 (Díptera: Cuterebridae, capturados com armadilha magoom na região metalúrgica do estado de Minas Gerais, Brasil Ocurrence of biological vectors of Dermatobia hominis (L. Jr.,1781(Diptera:Cuterebridae, captured by magoom trap in the matallurgic region, Minas Gerais, Brazil

    Directory of Open Access Journals (Sweden)

    Batista Zenón Rodríguez

    1997-12-01

    Full Text Available Através do uso da armadilha Magoam foram capturados 49.010 insetos no período de junho de 1995 a maio de 1996 no município de Pedro Leopoldo MG. Foram identificadas 10 ordens de insetos. Destes, 95,73% pertencem à ordem Díptera. Três famílias, Sepsidae, Syrphidae e Culicidae participaram com 24,65%, 18,53%, e 17,64% respectivamente dos 46.915 dípteros capturados. Dos três locais pesquisados, a mata (local 3 contribuiu com o maior número de insetos capturados correspondentes a 43,99% do total de espécimes colhidos. Os vetares biológicos de D. hominis (com postura aderida importantes por ordem de frequência foram Fannia spp, Musca domestica, Stomoxys calcitrans, Synthesiomyia nudiseta, Ophira sp., sendo esta última notificada pela primeira vez. O gênero Fannia spp. apresentou-se como de maior importância epidemiológica por sua maior frequência e distribuição nos locais de captura.From June 1995 to May 1996, 49,010 insects were captured in the municipality of Pedro Leopoldo, MG, Brazil, using the Magoom's trap. Ten orders were identified and 95.73% of them belong to the order Diptera. Three families, Sepsidae, Syrphidae, and Culicidae, represented 24.65%, 18.53%, and 17.64% respectively, of 46,915 diptera trapped. The bush contributed with the highest number of trapped insects (43,99% when comparsa with stable or pasture. The biological vectors of D. hominis (with adhered, eggs, in order of prevalence, were Fannia spp, Musca domestica, Stomoxys calcitrans, Synthesiomyia nudiseta, Ophira sp. This is the first identification of the genus Ophira sp. as biological vector of D. hominis. The genus Fannia was found to be the most important biological vector of D. hominis in Pedro Leopoldo, MG, Brazil, because of its high frequency and distribution in the three environment conditions searched.

  16. Susceptibility of Mixed Infection of Ureaplasma Urealyticum and Mycoplasma Hominis to Seven Antimicrobial Agents and Comparison with that of Ureaplasma Urealyticum Infection

    Institute of Scientific and Technical Information of China (English)

    黄长征; 刘志香; 林能兴; 涂亚庭; 李家文; 张德美

    2003-01-01

    Summary: In order to investigate the susceptibility of mixed infection of Ureaplasma Urealyticum(UU) and Mycoplasma Hominis (MH) to 7 kinds of antimicrobial agents and comparison with thatof UU infection in NGU patients, the in vitro susceptibility was determined by using microdilutionmethod. The positive results were analyzed. The results showed that the sequence of susceptibility to7 kinds of antimicrobial agents for both UU infection group and UU-MH mixed infection group wasalmost the same from the highest susceptibility to the lowest accordingly: Josamycin, Doxycycline,Minocycline, Sparfloxacin, Roxithromycin, Ofloxacin and Azithromycin. The total drug resistancerate for UU-MH mixed infection group (97.67 % ) was significantly higher than that for UU infec-tion group (44.67 %, P<0. 01). The highest drug resistance rate in UU group and UU-MH mixedinfection group was 31. 33 % (Ofloxacin) and 90. 48 % (Azithromycin) respectively. UU-MHmixed infection showed an increased drug resistance and changes of drug resistance spectrum.

  17. The Mycoplasma hominis P120 membrane protein contains a 216 amino acid hypervariable domain that is recognized by the human humoral immune response

    DEFF Research Database (Denmark)

    Nyvold, Charlotte Guldborg; Birkelund, Svend; Christiansen, Gunna

    1997-01-01

    domain. Based on restriction endonuclease cleavage patterns of the hypervariable domain the 18 isolates could be divided into four cases. Reactivity with both mAb 26.7D and pAb 121 confirmed these classes. The hypervariable, but not the constant, part of P120 was recognized by the human humoral immune...... and found to have a sequence identity of 91% with the gene of strain 7488. One hypervariable and two semivariable regions were detected. The epitope for mAb 26.7D was mapped to the hypervariable domain by expression of various parts of this domain in Escherichia coli using expression vector systems....... A polyclonal antiserum (pAb 121) generated against the hypervariable region of P120 from PG21 identified the P120 homologue in M. hominis PG21. Fusion proteins of the hypervariable and constant parts of the proteins were constructed and tested for reactivity with 21 human sera. Twelve sera reacted...

  18. Impuesto sobre la renta

    OpenAIRE

    Universitat de Barcelona. Grup d'Innovació Docent en Economia dels Impostos (GIDEI)

    2016-01-01

    Tema 7. Esquema de presentación del tema "Impuesto sobre la renta". Incorpora datos actuales, gráficos, cuadros y tablas. Esquema de presentació del tema "Impuesto sobre la renta". Incorpora dades actuals, gràfics, quadres i taules.

  19. Impuesto sobre sociedades

    OpenAIRE

    Universitat de Barcelona. Grup d'Innovació Docent en Economia dels Impostos

    2016-01-01

    Tema 8. Esquemas de la presentación del tema "Impuesto sobre sociedades". Incorpora datos actuales, gráficos, cuadros y tablas. Esquemes de la presentació del tema "Impuesto sobre sociedades". Incorpora dades actuals, gràfics, quadres i taules

  20. Digital signage sobre IP

    OpenAIRE

    Casademont Filella, Albert

    2012-01-01

    Projecte que versa sobre el disseny i la implementació d'un sistema de Digital Signage (Cartelleria Digital) sobre IP, creant un gestor que permeti controlar de forma remota tot un conjunt de petits dispositius connectats a pantalles que emeten continguts multimèdia com vídeos, imatges, feeds rss...

  1. Diferenciação morfológica das gônodas em larvas de Dermatobia Hominis (Diptera: Cuterebridae

    Directory of Open Access Journals (Sweden)

    Edy de Lello

    1984-06-01

    Full Text Available O trabalho descreve o desenvolvimento morfológico das gônadas, durante os três períodos larvais da Dermatobia hominis. Em larvas do 1° e 2° instar com a metodologia empregada, de dissecção sob lupa, foi impossível individualizá-las, mas elas aparecem nos cortes totais dessas larvas, como um pequeno aglomerado celular envolto por uma túnica acelular, medindo ao redor de 30 µm de diâmetro nas primeiras, e 54 µm nas segundas. Microscopicamente, apresentam células com dois tipos de núcleos, uns grandes arredondados e frouxos e outros menores, e ovóides; nas larvas mais jovens ambos os tipos nuclearesse misturam enquanto que nas mais velhas os maiores permanecem no interior e os menores se ajeitam ao redor da gônoda e entre os maiores. Anatomicamente a distinção entre testículo e ovário ocorre em larvas do 3° instar com peso a partir de 400mg.Morphological development of the gonads during the three larval instars of Dermatobia hominis is described. It was impossible to individualize the gonads on the first (L1 and second (L2 insars, by dissection under stereoscopic microscope. They appear in whole sections, as a small cellular cluster surrounded by a tunica, not exceeding 30 µm in diameter in L1, and 60 µm in L2. Histologically they show two types of cells, one with large round nuclei and other with small ovoid ones; these latter are located under the tunica and among the former. In the third instar the larvae grow considerably and gain weight from 50 to 900mg. In larva weighting between 100 to 200mg, under the stereoscope the gonads appear as a small dot; in larvae weighting between 250 to 350mg they appear as two transparent spheres of approximately 100 µm of diameter. The anatomical distinction between testicle and ovary ins only possible in larvae weighting 400 mg or more. This anatomical distinction is followed by rearrangements of the different types of cells.

  2. Investigation on Symbiosis of Mycoplasma hominis in Trichomonas vaginalis in Some Areas of Guizhou Province%贵州省部分地区阴道毛滴虫与人型支原体共生情况

    Institute of Scientific and Technical Information of China (English)

    匡贵榕; 衣凤芸; 杨宇箭; 程其会; 王丽娟; 吴家红

    2015-01-01

    目的:了解贵州地区阴道毛滴虫临床分离株与人型支原体共生情况。方法:以贵州部分地区女性阴道毛滴虫病患者阴道后穹隆分泌物中采集的阴道毛滴虫虫株,实验室达到纯培养后,采用Chelex-100的方法提取滴虫基因组DNA,设计人型支原体16S rDNA特异引物,进行聚合酶链式反应( PCR),琼脂糖凝胶电泳法对PCR产物进行分析,观察滴虫细胞内人型支原体共生情况。结果:从临床共采集到165株虫株中,83株获得纯培养;83株中人型支原体检测结果显示有47株阳性,阳性率为56.6%。结论:阴道毛滴虫虫株与人型支原体共生情况在我国贵州地区普遍性存在。%Objective:To investigate the symbiosis of Mycoplasma hominis in Trichomonas vaginalis in Guizhou Province. Methods:The clinical strains of T. vaginalis were collected from the hospitals and family planning service stations in Guiyang city and Anshun city. After pure culture in lab,the ge-nome DNA of T. vaginalis was extracted by Chelex-100,and 16S rDNA fragment of M. hominis was amplified by PCR with the specific primers. Agarose gel electrophoresis analysis was conducted and the symbiosis of M. hominis in T. vaginalis was observed. Results:A total of 165 strains were collected from the hospitals and family planning service stations,and 83 clinical strains attained pure culture,of which 47 strains were detected positively for M. hominis. The positive rate was 56. 6%. Conclusions:The symbiosis of M. hominis in T. vaginalis is prevalent in Guizhou province.

  3. Relationship of Mycoplasma Hominis and Bacterial Vaginosis%人型支原体与细菌性阴道病相关性的研究

    Institute of Scientific and Technical Information of China (English)

    叶千红; 尚红; 陈昕; 侯香圃; 张淑兰

    2001-01-01

    目的:初步探讨人型支原体(mycoplasm hominis,MH)与细菌性阴道病(bacterial vaginosis,BV)的相关性,探索我国BV的致病因子.方法:根据BV的诊断标准选择100名BV者,同时选择177名健康体检者做正常对照组,取阴道分泌物进行MH培养.检测结果经SAS 6.11统计软件包处理,计算χ2值,判断MH与BV的相关性.结果:BV者MH阳性检出率为43%,明显高于对照组(5.6%),统计学差异十分显著(P<0.001).结论:MH与BV有明显的相关性,可能是BV的致病因子之一.

  4. Todo sobre el neobarroco

    Directory of Open Access Journals (Sweden)

    Arturo Carrera

    2007-07-01

    Full Text Available Lo que hemos escuchado y atendido hasta aquí, hoy, ayer, sobre todo en la propuesta poética esbozada por Sergio Raimondi en su ensayo sobre Aldo Oliva, demuestra que las singularidades, su pasión, anulan subrepticiamente las nominaciones.Anoche en la televisión una orquesta sinfónica y un coro numeroso. Súbitamente, me hubiera gustado ser uno de esos intérpretes solitarios para sí, a pesar de la armonía, anónimos, a pesar del brillo sonoro… un violín, un triángulo… apenas… tres grillos… como ...

  5. Resumen estudios sobre tabaquismo

    OpenAIRE

    Gónzalez, M.M. (M.)

    2009-01-01

    Este documento se realizó para publicar en la página Web de la Gerencia de Atención primaria del Área III. Este documento es una recopilación de estudios sobre tabaquismo. Gerencia de Atención Primaria Área III

  6. Clinical Efficacy of Placenta Hominis Extract in the treatment of Childhood Vitiligo%紫河车提取物治疗儿童白癜风的临床对照研究

    Institute of Scientific and Technical Information of China (English)

    王涛; 许爱娥; 尉晓冬; 董东

    2011-01-01

    Objective:To observe clinical efficacy of Placenta Hominis extract, Human Placental Histosolution and Human Placenta lipopolysaccharide in treating with childhood vitiligo. Methods:To evaluate efficacy of Placenta Hominis extract, Human Placental Histosolution and Human Placenta Lipopolysaccharide combined infrared radiation treatment of childhood vitiligo 20 min twice a day, and the same times apply drugs 5min intervals to lesion. Results;The effective rate of Placenta Hominis extract, Human Placental Histosolution and Human Placenta Lipopolysaccharide was respectively 78.50% , 47.06% and 57. 14% , the markedly effective rate was respectively 38.32% , 23,53% and 38. 10%. After treatment, there were statistically significant differences between three drugs as regards to the efficiency ( P < 0.05 ). The effective rate and markedly effective rate of Placenta Hominis extract were higher than others. In addition, the effective rate of Placenta Hominis extract as high as 89.66% for the scalp and significant differences were observed between scalp and other pait(P<0.05). Conclusions:Three drugs combined infrared radiation has a good effect on childhood vitiligo, and the efficiency of Placenta Hominis extract is superior to Human Placental Histosolution and Human Placenta Lipopolysaccharide.%目的:观察紫河车提取物、人胎盘组织液及人胎盘脂多糖治疗儿童白癜风的临床疗效.方法:采用紫河车提取物、人胎盘组织液以及人胎盘脂多糖外用联合红外线照射治疗儿童白癜风,早晚各1次,每次红外线照射20min,每隔5min搽药水1次进行治疗,观察其疗效.结果:紫河车提取物有效率为78.50%,显效率38.32%;人胎盘组织液有效率47.06%,显效率23.53%;人胎盘脂多糖有效率57.14%,显效率38.10%,3种药物的总有效率之间有统计学差异(P<0.05),紫河车提取物组疗效明显优于其它两种药物.此外,紫河车提取物治疗头皮部白斑的有效率高达89.66%,

  7. Anti-breast cancer effects of live, heat-killed and cytoplasmic fractions of Enterococcus faecalis and Staphylococcus hominis isolated from human breast milk.

    Science.gov (United States)

    Hassan, Zubaida; Mustafa, Shuhaimi; Rahim, Raha Abdul; Isa, Nurulfiza Mat

    2016-03-01

    Development of tumour that is resistant to chemotherapeutics and synthetic drugs, coupled with their life-threatening side effects and the adverse effects of surgery and hormone therapies, led to increased research on probiotics' anticancer potentials. The current study investigated the potential of live, heat-killed cells (HKC) and the cytoplasmic fractions (CF) of Enterococcus faecalis and Staphylococcus hominis as anti-breast cancer agents. MCF-7 cell line was treated with 25, 50, 100 and 200 μg/mL each of live, HKC and CF of the bacteria; and cytotoxicity was evaluated for 24, 48 and 72 h using MTT assay. The morphological features of the treated cells were examined by fluorescence microscopy. The stage of cell cycle arrest and apoptosis were quantified by flow cytometry. The bacterial effect on non-malignant breast epithelial cell line, MCF-10A, was assessed using MTT assay for 24, 48 and 72 h. All the three forms of the bacteria caused a significant decrease in MCF-7 (up to 33.29%) cell proliferation in concentration- and time-dependent manner. Morphological features of apoptosis like cell death, cell shrinkage and membrane blebbing were observed. Flow cytometry analyses suggested that about 34.60% of treated MCF-7 was undergoing apoptosis. A strong anti-proliferative activity was efficiently induced through sub-G1 accumulation (up to 83.17%) in treated MCF-7 and decreased number in the G0/G1 phase (74.39%). MCF-10A cells treated with both bacteria showed no significant difference with the untreated (>90% viability). These bacteria can be used as good alternative nutraceutical with promising therapeutic indexes for breast cancer because of their non-cytotoxic effects to normal cells.

  8. Tesis sobre municipalismo libertario

    OpenAIRE

    Bookchin, Murray

    2014-01-01

    Históricamente, la teoría y la práctica social radical se han centrado sobre dos campos de la actividad social humana: el lugar de trabajo y la comunidad. A partir de la creación del Estado-nación y de la Revolución Industrial, la economía ha ido adquiriendo una posición predominante sobre la comunidad; y no sólo en la ideología capitalista, sino también en los diferentes socialismos, libertarios y autoritarios, que han ido apareciendo en el último siglo. Este cambio de posición del socialism...

  9. Escrito sobre el cuerpo

    Directory of Open Access Journals (Sweden)

    Antonio Malalana Ureña

    2013-09-01

    Full Text Available El catálogo, en una edición bilingüe español-inglés, es una pequeña guía de la exposición Shirin Neshat. Escrito sobre el cuerpo, muestra que se integra dentro de los actos de PHOTOESPAÑA13, el festival internacional de fotografía y artes visuales. El esquema del libro es sencillo y los textos son extremadamente divulgativos. El primero de ellos, Escrito sobre el cuerpo (pp. 10-15, cuyo autor es Octavio Zaya, comisario de la exposición, resume la trayectoria de esta intelectual, que "interactúa" con la fotografía y los formatos audiovisuales, y nos presenta la argumentación narrativa de los documentos expuestos, contextualizando la obra recopilada.

  10. Inferencias sobre Grafos

    Directory of Open Access Journals (Sweden)

    Sira M. Allende

    2002-05-01

    Full Text Available El estudio de un juego puede ser modelado asumiendo que solo algunas partidas son observadas. Entonces el árbol del juego debe ser estimado utilizando información muestral. Similarmente ocurre al obtener información sobre el comportamiento de las decisiones tomadas por individuos muestreados sobre un árbol de decisión teórico. Al considerar una medida de probabilidad que caracterice el comportamiento de redes aleatorias se puede obtener un estimado del árbol asociado. Este problema es el analizado en este trabajo. Algunos resultados experimentales obtenidos usando Recocido Simulado ilustran el procedimiento. Su papel en el estudio de modelos económicos y de mercadeo es discutido.

  11. Hemilucilia segmentaria (Fabricius, 1805 (Diptera: Calliphoridae as new biological vector of eggs of Dermatobia hominis (Linnaeus Jr., 1781 (Diptera: Oestridae in Reserva Biológica do Tinguá, Rio de Janeiro, Brazil

    Directory of Open Access Journals (Sweden)

    Marinho Clarissa Rezende

    2003-01-01

    Full Text Available The aim of this note was to record for the first time the finding of Hemilucilia segmentaria acting as biological vector of Dermatobia hominis, during a study of the diversity of Calliphoridae at Reserva Biológica do Tinguá, Rio de Janeiro, Brazil. The insects were captured using traps baited with chicken vicera, for a period of 28-30 h twice per month. In the period of one year, 1987 insects were collected, 7.5% of which belonged to the H. segmentaria; of these a female was captured in May 2001, carrying a mass of 20 eggs on the left side of its abdomen.

  12. Prevalence of Chlamydia trachomatis, Ureaplasma spp., Mycoplasma genitalium and Mycoplasma hominis among outpatients in central Greece: absence of tetracycline resistance gene tet(M over a 4-year period study

    Directory of Open Access Journals (Sweden)

    A. Ikonomidis

    2016-01-01

    Full Text Available A total of 301 men and women attending local urologists and gynaecologists in the state of Thessaly, central Greece, were tested for Chlamydia trachomatis, Ureaplasma spp., Mycoplasma genitalium and Mycoplasma hominis DNA. Investigation of the tet(M gene, which confers tetracycline resistance in these genera, was also performed. Low incidence of C. trachomatis and Mycoplasma spp. as well as high prevalence of Ureaplasma spp., especially among women, were found. The tet(M gene was absent in all cases, notably in a region where doxycycline administration remains the first therapeutic option unless special medical conditions direct otherwise.

  13. 阴道毛滴虫与人型支原体共生对铁氧还蛋白基因影响%Effects of the Symbiosis of Trichomonas Vaginalis with Mycoplasma Hominis on Ferredoxin Gene

    Institute of Scientific and Technical Information of China (English)

    刘晓东; 温雯静; 薛长贵

    2011-01-01

    We isolated 30 Trichomonas vaginalis for the PCR detection from the gynecological outpatients in the Affiliated Hospital of Zhengzhou University using the specific 16s rDNA primers of Mycoplasma hominis. The results showed that there were 25 cases of Mycoplasma hominis infection, with the infection rate of 83. 33%. This gave a clew that the symbiosis of Trichomonas vaginalis with Mycoplasma hominis may be of certain generality in China. We sequenced the ferredoxin gene of 10 Trichomonas vaginalis where 5 Mycoplasma hominis were positive and five negative, and found that the ferredoxin (Fd) gene of the 10 Trichomonas vaginalis were exactly the same. But compared to the genes in the GenBank. A comparative analysis of the gene revealed that there were 3 more ctg bases at the 200th position of encoding leucine, but this did not lead to changes in reading frame. The gene homology was 99%.%采用人型支原体16s rDNA的特异引物,对从郑州大学附属医院妇科门诊患者分离到的30株阴道毛滴虫进行PCR检测,结果有25株感染人型支原体,感染率为83.33%,这显示了阴道毛滴虫和人型支原体之间的共生关系在中国具有普遍性.并对10株阴道毛滴虫(5株人型支原体阳性和5株人型支原体阴性)的铁氧还蛋白(Fd)基因进行测序,探讨人型支原体对Fd基因的影响,结果发现10株阴道毛滴虫的Fd基因完全相同,但与GenBank中的基因进行比较分析,在第200位多出ctg三个碱基,编码亮氨酸,但并未导致读码框架改变.基因同源性为99%.

  14. 人型支原体实时荧光聚合酶链反应检测方法的初步建立%Establishment of real-time PCR assay to detect Mycoplasma hominis

    Institute of Scientific and Technical Information of China (English)

    张慧芳; 张建中; 赵飞

    2012-01-01

    目的 建立一种快速、灵敏和特异的人型支原体实时荧光聚合酶链反应(real-time PCR)检测技术.方法 依据人型支原体gap基因保守区域使用Beacon Designer 7.0软件设计引物和探针,建立人型支原体real-time PCR检测方法并优化.对优化后的方法进行实验室灵敏度、特异度和检测限评价,并与聚合酶链反应(PCR)进行比较.结果 该real-time PCR对于人型支原体的检测限为50 cfu,PCR检测限为5×103cfu,其检测灵敏度为PCR的100倍.所建立的检测方法对其他10种常见支原体、12种泌尿生殖道感染病原菌染色体及人类染色体的扩增均为阴性.结论 本研究建立的real-time PCR方法可灵敏、特异的检测人型支原体,有望用于临床标本检测.%Objective To develop a real-time PCR assay to detect Mycoplasma hominis. Methods By analyzing the gap gene of M. hominis, an optimized real-time PCR assay was designed. The specificity, sensitivity and detection limit of the assay were evaluated and compared with conventional PCR assay by using standard concentration DNA of M. hominis. Results The detection limit of the assay was about 50 cfu and the specificity of the assay appeared to be 100%. The sensitivity of this real-time PCR assay was 100 times higher than that of conventional PCR. Conclusion This real-time PCR assay might be a suitable method for the clinical detection of M. hominis.

  15. SCCmec 相关的 psm-mec 在血液来源人葡萄球菌中的基因定位%Genetic location of SCCmec-associated psm-mec in Staphylococcus hominis from blood culture

    Institute of Scientific and Technical Information of China (English)

    陈亮; 杨永长; 肖代雯; 喻华; 刘华; 黄文芳

    2015-01-01

    Objective To investigate the genetic location of SCCmec-associated psm-mec in Staphylococcus hominis isolated from blood culture,and to lay a foundation for further functional studies of psm-mec in Staphylococcus hominis.Methods 25 strains of Staphylococcus hominis isolated from positive blood culture were collected.mecA and psm-mec gene were amplified by PCR,and the SCCmec types were determined by the results of multiplex PCR assay.For analyzing the genetic location characteristic of psm-mec in SCCmec,three pair special PCR primers were used to measure mecR1/psm-mec,psm-mec/xylR and fudoh respectively.Results There were 21 strains of methicillin-resistant Staphylococcus hominis and 4 strains of methicillin-sensitive Staphylococcus hominis. The positive rate of psm-mec gene in methicillin-resistant Staphylococcus hominis was 47.6%,and no psm-mec gene was found in methicillin-sensitive Staphylococcus hominis.Among psm-mec positive strains,2 strains belonged to SCCmecⅢ,5 strains belonged to SCCmecⅢ-like,and 3 strains belonged to new SCCmec types.All of the 10 psm-mec positive strains were mecR1/psm-mec,psm-mec/xylR and fudoh gene positive.Conclusion SCCmec-associated psm-mec extensively exists in methicillin-resistant Staphylococ-cus hominis isolated from positive blood culture,which distributes mainly in typical SCCmecⅢ,SCCmecⅢ-like and new SCCmec types and locates between mecR1 and xylR gene.%目的:了解 SCCmec 相关的 psm-mec 基因在血液来源人葡萄球菌中的基因定位特征,为深入研究 psm-mec 基因在人葡萄球菌中的功能奠定基础。方法收集临床血培养分离的人葡萄球菌25株,通过 PCR 扩增 mecA 基因和 psm-mec 基因,多重 PCR 对耐甲氧西林人葡萄球菌 SCCmec 分型,PCR 扩增 mecR1/psm-mec 基因与 psm-mec/xylR 基因间隔序列及 fudoh 基因,探究 psm-mec 基因在 SCCmec 上的定位特点。结果 PCR 检测显示,21株为耐甲氧西林人葡萄球菌,psm-mec

  16. Mycoplasma hominis Symbiosis and Trichomonas vaginalis Metronidazole Resistance%人型支原体共生与阴道毛滴虫甲硝唑耐药性的关系

    Institute of Scientific and Technical Information of China (English)

    王频佳; 谢成彬

    2012-01-01

    目的 研究人型支原体(Mycoplasma hominis)的共生与阴道毛滴虫(Trichomonas vaginalis)甲硝唑耐药性的关系.方法 2010年11月至2011年7月,自四川省妇幼保健院妇科门诊患者生殖道分泌物中分离出160株阴道毛滴虫,用梯度浓度1 024、512、256……4、2和1 μg/ml甲硝唑分别处理该批虫株,以死亡率≥90%的最低浓度作为甲硝唑最小致死浓度(MLC).以160个阴道毛滴虫分离株中提取的DNA为模板,用PCR技术特异性扩增人型支原体16S rRNA基因,检测滴虫细胞内是否有人型支原体共生.对检出人型支原体DNA的分离株用32μg/ml多西环素清除支原体,比较清除前后甲硝唑MLC的变化.结果 160个阴道毛滴虫分离株中甲硝唑MLC为1~8μg/ml的占61.3%(98/160),16~32μg/ml的占26.3%(42/160),64~256μg/ml的占12.5%(20/160).PCR检测结果显示,有61株(38.1%)检出人型支原体DNA,其中MLC为1~8μg/ml的分离株检出率为13.3%(13/98),16~32μg/ml的分离株检出率为73.8%( 31/42),64~256μg/ml的分离株检出率为85.0%(17/20),不同MLC范围的分离株人型支原体检出率差异有统计学意义(P<0.01).用多西环素处理后,61株中仅有8株支原体被清除,清除前后甲硝唑MLC无明显变化.结论 四川地区的阴道毛滴虫分离株对甲硝唑表现出一定程度的耐药性,人型支原体的共生可能与之有关,但尚未发现直接证据.%Objective To investigate the relation of Mycoplasma hominis symbiosis and the resistence of Trichomonas vaginalit to metronidazole. Methods From November 2010 to July 2011, 160 isolates of T. Vaginalis were collected from the genital tract secretion of gynecological out-patients at the Sichuan Provincial Hospital for Women and Children. The minimum lethal concentration (MLC) to metronidazole of these isolates was determined by an in. Vitro sensitivity assay with different concentration gradients of metronidazole (from 1 to 1 024 μg/ml), and M

  17. Double triplex real-time PCR assay for simultaneous detection of Staphylococcus aureus, Staphylococcus epidermidis, Staphylococcus hominis, and Staphylococcus haemolyticus and determination of their methicillin resistance directly from positive blood culture bottles.

    Science.gov (United States)

    Kilic, Abdullah; Basustaoglu, A Celal

    2011-12-01

    We developed and validated here a double triplex real-time PCR assay to simultaneously detect and identify Staphylococcus aureus, Staphylococcus epidermidis, Staphylococcus hominis, Staphylococcus haemolyticus and their methicillin resistance in a single reaction directly from Gram-positive cocci-in-clusters (GPCs)-positive blood culture bottles. From August 15, 2009 through February 15, 2010, 238 GPC-positive samples were collected and identified by conventional methods as 11 methicillin-resistant S. aureus (MRSA), 28 methicillin-susceptible S. aureus (MSSA), 176 MR coagulase-negative staphylococci (MRCoNS), 21 MSCoNS and two Enterococcus faecalis. The double triplex real-time PCR assay was targeted and detected tuf, nuc and mecA genes in the first tube and atlE, gap and mvaA genes in the second tube which could be run simultaneously. The detection limit of the assay was found at 10(3) CFU/ml for the atleE gene, 10(4) CFU/ml for the mva gene and 10(5) CFU/ml for gap, nuc, mecA and tuf genes based on seeding experiments. All Staphylococcus species except two S. epidermidis were correctly identified by the assay. The double triplex real-time PCR assay quickly and accurately detects S. aureus, S. epidermidis, S. hominis and S. haemolyticus and their methicillin resistance in a single reaction directly from positive blood culture bottles within 83 min.

  18. Puente sobre el Rin

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    Gortz, Wilhelm

    1961-12-01

    Full Text Available Del total de 35 proyectos presentados al concurso del puente para carretera sobre el Rin, entre Duisburg-Ruhrort y Homberg, sólo dos proposiciones de la Demag fueron consideradas: Una con tablero suspendido por vientos que parten de dos torres, y otra, con suspensión por cables flexibles que se apoyan en dos torres y anclaje en la misma estructura. Por razones netamente estéticas fue elegida la propuesta Demag de puente suspendido por cables, aunque la solución con vientos hubiera sido la más económica, teniendo en cuenta las condiciones existentes.

  19. Saber sobre la radio

    OpenAIRE

    Mata, María Cristina; Universidad Nacional de Córdoba. Argentina

    1998-01-01

    La autora propone en este artículo repensar los fundamentos sobre los cuales se estructuran las asignaturas y talleres de radio en las escuelas de Comunicación Social en Latinoamérica, con el proposito de romper la escisión  entre teoría/práctica y de no violentar su complejidad: en la radio, entendida como práctica comunicativa, se juega en primer lugar una "red de vinculaciones e intercambios" en condiciones privilegiadas para la dialoguicidad. En un segundo lugar, asociado al desarrollo de...

  20. Excertos sobre o sedentarismo

    OpenAIRE

    Alexandre Palma; Murilo Mariano Vilaça; Monique Ribeiro de Assis

    2014-01-01

    O sedentarismo tem sido tratado como algo perfeitamente determinável, objetivo e, portanto, que pode ser apreendido. Por outro lado, o conceito de sedentarismo ainda permanece questionável e, por conseguinte, os instrumentos mostram-se inconsistentes. Neste sentido, o objetivo do presente estudo foi mostrar que diferentes discursos de verdade vêm concorrendo por esse conceito, o que nos motiva a pensar qual é a verdade ou, até mesmo, se há alguma verdade sobre ele. Após apresentarmos diferent...

  1. Sobre historia mundial hoy

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    Vera Weiler

    2010-08-01

    Full Text Available Los procesos de globalización son una realidad; su enorme impacto sobre la vida humana los ha convertido en un tema recurrente sobre el que se escribe y se comenta a diario también en Colombia. Los libros que de ellos tratan, ante todo los que 10 hacen en tono crítico, baten records en la industria editorial en todo el mundo. Las expectativas que acerca del futuro abrigan cientos de millones de personas se relacionan con 10 que ellas esperan, para bien o para mal, de la globalización. Las posturas que los gobernantes ocupan al respecto en el mundo son, cuando menos de aceptación, generalmente de activa participación en pos de la globalización. Por un lado, crece la preocupación y se multiplican las protestas; por el otro, dominan los razonamientos de los especialistas en materia de maximización de los rendimientos de los capitales que presentan la globalización, al estilo que se viene imponiendo, como el camino ineludible del gobierno universal de la eficiencia. ¿Tienen que decir algo los historiadores frente a todo esto?.

  2. Sobre la tragedia griega

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    Mauricio Vélez Upegui

    2015-01-01

    Full Text Available Luego de caracterizar la tragedia griega clásica como expresión de un arte ciudadano que participa por igual de un carácter festivo, cierto régimen discursivo y una clara inscripción físico-espacial, el texto se demora en considerar tres aspectos fundamentales de esta forma dramática inventada por los griegos, a saber: una exposición sobre las fuentes orales míticas de las que los autores echan mano para componer la historia de cada una de las piezas que son llevadas a escena al amparo de un espíritu agonal; una explicación del funcionamiento formal de la tragedia, basado en la alternancia de partes cantadas (coro y partes recitadas (héroe; y, finalmente, una acotación sobre el sentido –interno y externo– que la acción dramática posee, en tanto elemento articulador de la trama.

  3. Laboratory Model for Cryptosporidium hominis IbA19G2 Subtype in Mongolian Gerbils%人隐孢子虫IbA19G2基因亚型蒙古沙鼠感染模型的建立

    Institute of Scientific and Technical Information of China (English)

    王强; 杨楠; 菅复春; 王荣军; 宁长申; 张龙现

    2011-01-01

    人隐孢子虫Cryptosporidium hominis是人类的主要感染虫株,目前已获取且传代保存的国内分离株较少,在生物学特性研究也未见报道.在本试验自感染前第10天起至排卵囊结束后14天,采用胃管灌服地塞米松(0.75 mg/(只·2天))抑制蒙古沙鼠免疫力,对国内C.hominis Ib亚型分离株生物学特性进行研究.结果显示,感染后第3天,感染组有卵囊排出,感染后第6天出现高峰期,此后逐渐下降直至感染后第10或11天排卵囊结束.感染前后基于18S rRNA和gp60基因分析,表明遗传学无差异.建立了持续稳定的C.hominis Ib亚型分离株啮齿动物模型,为C.hominis卵囊扩增和进一步研究其生物学特性等提供了重要的参考依据.%Researches have sbown that Cryptosporidium hominis is one of the main Cryptosporidium parasites for human cryptosporidiosis in China. However, only a few isolates of C. hominis were obtained and aerially sub-cultivated in laboratory up to date, so the biological characteristics of C. hominis remaina unreported in our country. In the present work, Mongolian gerbils (Meiiones unguiculataus) were orally inoculated with 0. 75 mg of dexamethasone/animal by using gastric tube drench every two days from the 1Oth day before oocyst ingestion. The results showed that the gerbils started to shed oocysts in the third day after inoculation ( DAI) , the number of shed oocysts increased obviously, reached the peak value at the 6th DAI,and then dropped graduaUy until lOth or llth DAI. The 18S rRNA and gp60 gene analysis performed before and after inoculation revealed no genetic difference of C. hominis oocysts. A stable rodent model of C. homiais Ib subtype infection has been successfully established, which may be an important value for the further study on the biological characteriatica of C. hominis.

  4. Excertos sobre o sedentarismo

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    Alexandre Palma

    2014-09-01

    Full Text Available O sedentarismo tem sido tratado como algo perfeitamente determinável, objetivo e, portanto, que pode ser apreendido. Por outro lado, o conceito de sedentarismo ainda permanece questionável e, por conseguinte, os instrumentos mostram-se inconsistentes. Neste sentido, o objetivo do presente estudo foi mostrar que diferentes discursos de verdade vêm concorrendo por esse conceito, o que nos motiva a pensar qual é a verdade ou, até mesmo, se há alguma verdade sobre ele. Após apresentarmos diferentes possibilidades de compreender o sedentarismo, através do uso de aforismos, entendemos que postular um discurso como verdade talvez apenas signifique que as pessoas nele creem e que há uma vontade de verdade como uma vontade voltada para o poder.

  5. Sobre el Portugal ilustrado

    OpenAIRE

    Santolaya Heredero, Laura

    1992-01-01

    El mismo título invita a preguntarse, a modo de introducción, en qué consistió la Ilustración en Portugal. Me refiero a las prácticas, sin pretender entrar en un debate filosófico. Desde luego, las prácticas políticas y económicas de los Estados del XVIII poco tuvieron que ver con las ideas de los filósofos de la Ilustración. El fenómeno de la Ilustración se aprovechió de una propaganda anecdótica para hacer suponer a los lectores de libros sobre ella que en los diversos ...

  6. Sobre multifunciones Fuzzy

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    Renato César Scarparo

    2002-01-01

    Full Text Available En este trabajo se presentan y demuestran algunos resultados de D.T. Luc y C, Vargas referentes a multifunciones con dominio y blanco en espacios vectoriales topológicos de Hausdorff sobre R, como así mismo se explícita el concepto de multifunción fuzzy de acuerdo a Papageogiou, y se demuestran dos teorema de S. S. Chag, con respecto a las multifunciones fuzzy, proposiciones todas estas, que integran una línea de resultados necesarios para la demostración de desigualdades variacionales para multifunciones fuzzy, a su vez necesarias, para la extensión fuzzy de conocido teorema de Walras.

  7. Sobre la muerte voluntaria

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    Juan Álvarez-Cienfuegos Fidalgo

    2012-03-01

    Full Text Available El problema de cómo morir en nuestra sociedad se ha convertido en una inquietud para los ciudadanos y la reflexión moral. Especialmcnte por dos razones: por el desarrollo de la tecnología médica y por el hecho de que la mayor parte de los fallecimientos tienen lugar fuera del hogar, donde tradicionalmente ocurra el deceso. El presente artículo tiene como objetivo acercarse a esta cuestión. Para ello, se parte de la autonomía del ciudadano, con derecho a decidir sobre lo que considera cuál sea su manera de vivir y de morir. A tal fin, se hace una breve introducción sobre el dolor, el suicidio y la muerte, desde un punto de vista antropológico, pasando, después, a mostrar las cuestiones relacionadas con el problema de la eutanasia, donde la atención se dirigirá, ante todo, al enjuiciamiento crítico del tenso equilibrio en el que se mantienen dos principios fundamentales de la bioética: el de no maleficencia y el de autonomía del ciudadano.The problem of how to die in modern society has become a concern for both the citizen and moral reflection for two reasons in particular: due to developments in medical technology, and because most deaths occur outside the honre, where they traditionaÍlY took place. This paper addresses this issue, starting from citizen autonomy, the right to decide on what s/he considers to be his or her way of living and dying. To this end, a brief introduction considers pain, suicide and death from an anthropological viewpoint. Questions related to the problem of euthanasia are then exposed, with particular attention to, above all the critical judgement of the tense balance in which two fundamental principies of bioethics are held, the principle of non-maleficence and that of citizen autonomy.

  8. Debate sobre el parto (normal).

    OpenAIRE

    Vidal Puèrtolas, Adela

    2008-01-01

    El artículo pretende una reflexión sobre la asistencia al parto, desde el punto de vista de una comadrona. A raíz de la apuesta oficial sobre el cómo para humanizar la asistencia obstétrica hospitalaria, se plantea un análisis de las implicaciones profesionales y los matices culturales que influyen sobre la vivencia de la experiencia del parto. La importancia de la psico-profilaxis en la preparación al parto como vehículo terapéutico de acercamiento a la realidad y de ...

  9. Debate internacional sobre pobreza

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    Neritza Alvarado Chacín

    2016-01-01

    Full Text Available El objetivo del artículo es organizar la proliferación de ideas en torno de la pobreza, a menudo dispersas, difusas y confusas en la literatura, bosquejando sistematizadamente un marco teórico referencial útil al abordaje científico de este fenómeno, en investigaciones teóricas y aplicadas, así como en la enseñanza y discusión de tales temas en el ámbito universitario. Para ello, se compendian, organizan y clasifican diversos enfoques sobre la pobreza, según los criterios presentes en los planteamientos de autores e instituciones relevantes que han contribuido a la reflexión desde distintas lecturas, teniendo en cuenta que no existe consenso en torno a una definición. La investigación es exploratoria-documental. Se aplican las técnicas del arqueo electrónico y físico de la información, el fichaje computarizado y la reducción de la misma en tablas de contenido. Se precisa el alcance de las mismas en el tiempo, según la evolución conceptual que han experimentado en el debate internacional. Se señalan algunas ventajas y desventajas de los principales enfoques, que han sido reconocidas en el mismo debate.

  10. Research concerning intestinal parasitosis in infant population discovered in two clinic laboratories from Oradea (Romania

    Directory of Open Access Journals (Sweden)

    Daniel-Dumitru PORTELECHI

    2006-05-01

    Full Text Available Considering the research made and the data offered by the specialists in parasitology from Oradea’s no.1 Polyclinic and Children’s Hospital, we have established that children from 1 to 18 years old have been found infected with 7 species of human parasites. Giardia lamblia, Blastocystis hominis, Ascaris lumbricoides, Oxyurus vermicularis, Trichurus trichiura, Hymenolepis nana, Hymenolepis diminuta.

  11. Parasites of Periplaneta americana linnaeus "domestic cockroach" from Ica.

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    Mary Fernádez B.

    2014-06-01

    Full Text Available 244 specimens of Periplaneta americana Linnaeus from 13 localities of Ica were studied. Nematodes and protozoa were identified. They are Lophomonas blattarum, Leptomonas sp., Leidynema appendiculatum and Hammerschmidtiella diesingi. Also, parasites of man were found, three of which are pathogenic: Giardia lamblia, Blastocystis hominis and Cryptosporidium sp.

  12. Is real-time PCR-based diagnosis similar in performance to routine parasitological examination for the identification of Giardia intestinalis, Cryptosporidium parvum/Cryptosporidium hominis and Entamoeba histolytica from stool samples? Evaluation of a new commercial multiplex PCR assay and literature review.

    Science.gov (United States)

    Laude, A; Valot, S; Desoubeaux, G; Argy, N; Nourrisson, C; Pomares, C; Machouart, M; Le Govic, Y; Dalle, F; Botterel, F; Bourgeois, N; Cateau, E; Leterrier, M; Le Pape, P; Morio, F

    2016-02-01

    Microscopy is the reference standard for routine laboratory diagnosis in faecal parasitology but there is growing interest in alternative methods to overcome the limitations of microscopic examination, which is time-consuming and highly dependent on an operator's skills and expertise. Compared with microscopy, DNA detection by PCR is simple and can offer a better turnaround time. However, PCR performances remain difficult to assess as most studies have been conducted on a limited number of positive clinical samples and used in-house PCR methods. Our aim was to evaluate a new multiplex PCR assay (G-DiaParaTrio; Diagenode Diagnostics), targeting Giardia intestinalis, Cryptosporidium parvum/Cryptosporidium hominis and Entamoeba histolytica. To minimize the turnaround time, PCR was coupled with automated DNA extraction (QiaSymphony; Qiagen). The PCR assay was evaluated using a reference panel of 185 samples established by routine microscopic examination using a standardized protocol including Ziehl-Neelsen staining and adhesin detection by ELISA (E. histolytica II; TechLab). This panel, collected from 12 French parasitology laboratories, included 135 positive samples for G. intestinalis (n = 38), C. parvum/C. hominis (n = 26), E. histolytica (n = 5), 21 other gastrointestinal parasites, together with 50 negative samples. In all, the G-DiaParaTrio multiplex PCR assay identified 38 G. intestinalis, 25 C. parvum/C. hominis and five E. histolytica leading to sensitivity/specificity of 92%/100%, 96%/100% and 100%/100% for G. intestinalis, C. parvum/C. hominis and E. histolytica, respectively. This new multiplex PCR assay offers fast and reliable results, similar to microscopy-driven diagnosis for the detection of these gastrointestinal protozoa, allowing its implementation in routine clinical practice.

  13. Sobre Humanismo y Medicina

    Directory of Open Access Journals (Sweden)

    Adolfo de Francisco Zea

    2000-12-01

    Full Text Available

    En esta disertación sobre “Humanismo y Medicina”, título del libro que tuve ocasión de publicar hace un par de años, destacaré algunas de las relaciones que existen entre la medicina y las bellas artes, concretamente la música y la pintura. La Medicina tiene como objeto de su acción al Hombre. Definida como la ciencia y el arte de prevenir, curar o aliviar la enfermedad, no tiene como meta la simple prolongación de la vida biológica.

    Su tarea consiste en postergar la muerte, prevenir o reducir los padecimientos y las deformidades, curar las enfermedades, ampliar las capacidades biológicas y psicológicas de los seres humanos y ocuparse de los sufrimientos de las personas. Para Tristam Engelhardt, es la Medicina de las personas.

    Se concibe a la persona humana como el individuo de conciencia reflexiva normal que es a la vez miembro pleno de la comunidad, con los deberes y derechos que presuponen su pertenencia a ella. A esta acepción de carácter moral de la persona humana, que corresponde a lo que se llama persona “en sentido estricto”, se agrega la acepción social, que hace relación con aquellas personas a las que se otorgan prácticamente los mismos derechos, como es el caso de los niños, de los individuos que perdieron el carácter de personas por padecer de lesiones cerebrales o demencia, y de aquellos que nunca llegarán a serlo como los retrasados mentales profundos.

    Al sostener que no todos los seres humanos son personas “en sentido estricto”, la Bioética que expone Engelhardt impone, sin embargo, como obligación moral, tratar a estos últimos con pleno respeto y comprensión. Lo que es importante acerca de nosotros mismos como seres humanos, es el hecho real de que seamos personas; no simplemente nuestra pertenencia al género Horno sapiens como tal.

    Para Víctor Frankl, la persona humana es de carácter espiritual y en tal virtud, es diferente del organismo psicofísico cuya

  14. Acueducto pretensado sobre el Holme

    Directory of Open Access Journals (Sweden)

    Editorial, Equipo

    1958-04-01

    Full Text Available Breve descripción del paso de una canalización de 0,60 m de diámetro, destinada al abastecimiento de aguas, sobre el río Holme (Inglaterra. El puente formado tiene dos vigas de hormigón pretensado, cuyas armaduras se componen de barras McCall.

  15. Blastocistosis y otras infecciones por protozoos intetinales en comunidades humanas ribereñas de la cuenca del rio Valdivia, Chile Blastocystosis and other intestinal protozoan infections in human riverside communities from - Valdivia River Basin, Chile

    Directory of Open Access Journals (Sweden)

    Patricio Torres

    1992-12-01

    Full Text Available Entre marzo y octubre de 1987 se examinaron muestras coprológicas de 970 personas (20, 9% de la población pertenecientes a 209 grupos familiares de las comunidades ribereñas de la cuenca del rio Valdivia, Chile; con el propósito de determinar las prevalencias de infección por Blastocystis hominis y otros protozoos intestinales para establecer relaciones con la edad y sexo de los hospedadores, saneamiento ambiental y porcentaje de individuos infectados por grupo familiar. Un 72, 5% de las personas presentó una o más especies de protozoos intestinales. La mayor prevalencia se registró para B. hominis (61, 8%, que se incrementó con la edad del hospedador al igual que en las infecciones por Endolimax nana y Entamoeba coli. No se demostró asociación entre el sexo del hospedador y la prevalencia de infección por B. hominis y otras especies de protozoos. La prevalencia de B. hominis fue mayor en individuos que habitaban viviendas cuya disposition de excrementos era no sanitaria. Más del 60% de los integrantes de los grupos familiares presentaron infección por B. hominis en el 53, 1% de las familias encuestadas en contraposición al 2,4%-21,8% observado en infecciones por otros protozoos. El examen de 45 muestras de excrementos de cerdos, reveló infección por Blastocystis en el 22,2% de estos animales.Between March and October 1987, the prevalence of infection by Blastocystis hominis and other intestinal protozoan, their relationship with the age and sex of the hosts, and the percentage of in fected persons in family groups were determined in riverside communities of Valdivia River Basin, Chile. One or more intestinal protozoan species were determined in 72.5% of the examined persons. The prevalence was greater for B. hominis (61.8%. The prevalences of B. hominis, Endolimax nana and Entamoeba coli were greater in relation to the age of the host. The sex of the host and prevalence of infections by B. hominis and other species of

  16. Breve informe sobre la coca

    Directory of Open Access Journals (Sweden)

    Lauro Hinostroza García

    1985-12-01

    Full Text Available El presente informe se integra en la investigación dirigida por el doctor Juan Ossio, por encargo de la Organización de los Estados Americanos (O.E.A. sobre “EFECTOS SOCIALES, CULTURALES, MÉDICOS Y ECONÓMICOS DEL USO TRADICIONAL DE LA COCA EN POBLACIONES DEL ANTIPLANO Y SIERRA DEL PERÚ, EN ESPECIAL DE LAS POBLACIONES INDÍGENAS”. Expone tópicos sobre la hoja de coca en la cultura andina: criterios clasificatorios, mitos, canciones y una muestra de qatipay o seguimiento. Este último es una práctica ritual importante para el campesinado andino, orienta el presente, pasado y futuro de toda su actividad. Cada hoja, por su forma, posición y tamaño, develará los roles sociales que ha de asumir, es decir los misterios del tiempo.

  17. Sobre la verdad en periodismo

    Directory of Open Access Journals (Sweden)

    Julio Estremadoyro Alegre

    2011-05-01

    Full Text Available Cuando un periodista informa sobre un desastre natural, con granpérdida de vidas, las autoridades y las fuentes de primera manodan cifras que son difundidas como verdades del suceso. Sinembargo, y la experiencia lo confirma, al día siguiente la cifraspueden haber cambiado y, por lo tanto, la verdad informativa esotra. Y así, sucesivamente, cada día habrá novedades y nuevas cifras verdaderas.

  18. Sobre el Kelsen de Ferrajoli

    OpenAIRE

    Ruiz Manero, Juan

    2015-01-01

    El objeto de este trabajo es el de dar cuenta de las críticas a Kelsen contenidas en el último libro, todavía inédito, de Luigi Ferrajoli, Diritto e lógica. Dieci aporie nell'opera di Hans Kelsen, y formular algunas críticas a las tesis sostenidas en él por el propio Ferrajoli. Entre las críticas que el autor considera que cabe dirigir a este respecto a Ferrajoli, las principales afectan al carácter exclusivamente interno de la mirada ferrajoliana sobre el derecho, al carácter siempre opinabl...

  19. Manual de lecciones sobre objetos

    OpenAIRE

    2010-01-01

    Pautas para la enseñanza inicial con niños, apoyado con Objetos. Traducido por S. Bond (Profesor de la Universidad Nacional). Contiene: Desarrollo de la observación - Desarrollo de las ideas de forma - Ejercicios preliminares para enseñar a los niños a observar formas - Desarrollo de las ideas de dibujo -  de color - de número - de tamaño - de peso -  de sonido -  sobre el cuerpo humano - de lugar - morales

  20. Sobre el contenido del despertar

    OpenAIRE

    Badr, Marisa

    2013-01-01

    El presente trabajo tiene como propósito situar algunas referencias, sobre lo que Freud denomina “la perturbación” en los sueños. De este modo, intentaremos situar algunas consideraciones respecto a los sueños de angustia como principal antecedente del fracaso de la función del sueño y del principio del placer como principio rector del aparato anímico. Tomaremos como punto de partida los sueños de angustia, en “La interpretación de los sueños” de Sigmund Freud.

  1. Generalidades sobre las fibras artificiales

    OpenAIRE

    González Salcedo, Luis Octavio

    2012-01-01

    Las fibras han sido utilizadas como materia prima en la elaboración de otros productos, como telas, papel, artesanías, entre otros, y como material de refuerzo en diversas matrices cerámicas y metálicas, con el fin mejorar o ganar propiedades, en materiales compuestos denominados composites. Las fibras pueden ser clasificadas de acuerdo con su origen, en fibras naturales y fibras artificiales. Una amplia exploración sobre el uso de fibras vegetales ha sido realizada, sin embargo su uso ...

  2. Prototipo de RINA sobre Ethernet

    OpenAIRE

    Bergesio, Leonardo

    2013-01-01

    Este PFM se enmarca dentro del trabajo a realizar por el proyecto IRATI. IRATI es un proyecto STReP (Specific Targeted Research Project) financiado por la Unión Europea dentro del programa FP7 (Seventh Framework Programme for Research and Technological Development). El objetivo general de IRATI es conseguir una mayor compresión y exploración de RINA. El trabajo que se reportará en este PFM es (aproximadamente) la primera fase del diseño y desarrollo de un prototipo de RINA sobre Ethernet en e...

  3. Algunas conjeturas sobre fracciones egipcias

    OpenAIRE

    Mora, Lyda Constanza; Lima, Isaac

    2003-01-01

    A continuación se presentan algunas conjeturas sobre las fracciones, con base en el tratamiento que los Egipcios hacían en antigüedad. En la primera parte se hace una introducción a las fracciones unitarias, y la representación de fracciones con numerador dos por medio de la suma de fracciones unitarias distintas. En la segunda parte, se presentan algunas de las conjeturas halladas a partir de la determinación de regularidades presentes en adiciones entre fracciones cuya suma es una fracción ...

  4. Desarrollo de aplicaciones sobre SCTP

    OpenAIRE

    López Aragoneses, Jorge

    2009-01-01

    En el año 2000, el grupo SIGTRAN definió un nuevo protocolo de capa de transporte conocido como Stream Control Transmission Protocol (SCTP) pensado, inicialmente, para transportar señalización telefónica SS7 sobre IP. La intención fue proveer en IP algunas de las características de confiabilidad de SS7. Por su versatilidad, más tarde, se ha propuesto utilizarlo en otras áreas. Para tal fin, se ha realizado este proyecto fin de carrera, en el cual se estudiarán las características del protocol...

  5. Relationship between ParE gene mutation and fluoroquinolones resistance in Mycoplasma hominis%人型支原体ParE基因突变与耐氟喹诺酮类药物的关系

    Institute of Scientific and Technical Information of China (English)

    罗永慧; 段颖卿; 甘雪华; 王旭菲; 舒向荣

    2014-01-01

    目的:探讨人型支原体对氟喹诺酮类药物的耐药机制。方法自临床分离的8株耐氟喹诺酮类药物的人型支原体(Mh),对其ParE基因PCR扩增后进行测序分析,与基因库中的野生型菌株MHPG21基因序列比对,分析ParE基因突变位点与菌株耐氟喹诺酮类药物的关系。结果与野生株MHPG21对比,6株检出ParE基因所编码的氨基酸残基发生D 426→N变异。结论 Mh临床分离株对氟喹诺酮类药物的耐药可能与ParE基因所编码的氨基酸残基D 426→N变异有关。%Objective To explore the fluoroquinolones resistance meachanisms of Mycoplasma hominis (Mh). Methods A total of 8 strains of Mh with different fluoroquinolones resistance phenotype were analyzed, the ParE gene was amplified and sequenced. The sequencing results of ParE gene were compared with that in the wild type strain PG21, then analyzed the relation between ParE gene mutation and fluoroquinolones resistance. Results Compared with wild strain PG21, ParE gene in 6 strains got a D426→N mutation. Conclusion The fluoroquinolones resistance of Mh might be associate with ParE gene D426→N mutation.

  6. Phenotypic, Genotypic, and Antimicrobial Characteristics of Streptococcus halichoeri Isolates from Humans, Proposal To Rename Streptococcus halichoeri as Streptococcus halichoeri subsp. halichoeri, and Description of Streptococcus halichoeri subsp. hominis subsp. nov., a Bacterium Associated with Human Clinical Infections.

    Science.gov (United States)

    Shewmaker, P L; Whitney, A M; Humrighouse, B W

    2016-03-01

    Phenotypic, genotypic, and antimicrobial characteristics of six phenotypically distinct human clinical isolates that most closely resembled the type strain of Streptococcus halichoeri isolated from a seal are presented. Sequencing of the 16S rRNA, rpoB, sodA, and recN genes; comparative whole-genome analysis; conventional biochemical and Rapid ID 32 Strep identification methods; and antimicrobial susceptibility testing were performed on the human isolates, the type strain of S. halichoeri, and type strains of closely related species. The six human clinical isolates were biochemically indistinguishable from each other and showed 100% 16S rRNA, rpoB, sodA, and recN gene sequence similarity. Comparative 16S rRNA gene sequencing analysis revealed 98.6% similarity to S. halichoeri CCUG 48324(T), 97.9% similarity to S. canis ATCC 43496(T), and 97.8% similarity to S. ictaluri ATCC BAA-1300(T). A 3,530-bp fragment of the rpoB gene was 98.8% similar to the S. halichoeri type strain, 84.6% to the S. canis type strain, and 83.8% to the S. ictaluri type strain. The S. halichoeri type strain and the human clinical isolates were susceptible to the antimicrobials tested based on CLSI guidelines for Streptococcus species viridans group with the exception of tetracycline and erythromycin. The human isolates were phenotypically distinct from the type strain isolated from a seal; comparative whole-genome sequence analysis confirmed that the human isolates were S. halichoeri. On the basis of these results, a novel subspecies, Streptococcus halichoeri subsp. hominis, is proposed for the human isolates and Streptococcus halichoeri subsp. halichoeri is proposed for the gray seal isolates. The type strain of the novel subspecies is SS1844(T) = CCUG 67100(T) = LMG 28801(T).

  7. Blanco sobre blanco sobre blanco: Oiticica / Malevich / Nietzsche

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    Sérgio Martins

    2014-09-01

    Full Text Available El blanco sobre blanco, de Malevich, es una presencia fuerte en los escritos de Hélio Oiticica durante los setenta. Este artículo investiga los diferentes momentos del enlace de Oiticica con Malevich y el papel de esa noción en su pensamiento, articulándola a la referencia igualmente importante de Oiticica a John Cage y a la filosofía de Nietzsche. Así, es posible percibir tanto la centralidad del escrito para Oiticica en esta década, como la singularidad del modo de enunciación en él contenido, que puede ser descrito como aquel de un escrito manifiesto.

  8. Recursos y enlaces sobre comunidades de aprendizaje

    OpenAIRE

    Ferrer Esteban, Gerard

    2005-01-01

    Obras y artículos sobre el proyecto Comunidades de Aprendizaje desarrollado en el Estado español. Tesis doctorales sobre comunidades de aprendizaje. Fundamentación teórica de las comunidades de aprendizaje. Sitios en Internet relacionados con las comunidades de aprendizaje y proyectos afines de desarrollo comunitario en contextos escolares.

  9. As monografias sobre plantas medicinais

    Directory of Open Access Journals (Sweden)

    Valdir F. Veiga Junior

    2008-09-01

    Full Text Available As monografias sobre plantas medicinais ou drogas vegetais contêm informações para atender não somente aos órgãos de regulamentação, mas também às empresas industriais farmacêuticas e farmácias, e mesmo ao público consumidor. Este artigo revê as monografias da Comissão E, do American Botanical Council, ESCOP, PDR e da Organização Mundial de Saúde em suas origens, objetivos e formatos. Duas publicações recentemente organizadas pela FIOCRUZ do Rio de Janeiro e as contribuições da Farmacopéia Brasileira também são avaliadas.

  10. Puente sobre el rio Lay

    Directory of Open Access Journals (Sweden)

    Editorial, Equipo

    1959-11-01

    Full Text Available El puente sobre el río Lay, salva el río formando un ángulo de 69° con la dirección de éste. Situado en el término municipal de la Reorthe (Francia, sus características principales se concentran en sus tres vigas pretensadas, de 30 m de longitud, prefabricadas, siguiendo los procedimientos Preyssinet, proyectadas por la Société Technique pour l'Utilisation de la Précontrainte, que también se encargó de la redacción del proyecto general de ejecución, cuya realización se encomendó a la empresa constructora Dodin, y de los métodos empleados para la colocación de las vigas en obra.

  11. Escenarios sobre el orden internacional

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    Luis Dallanegra Pedraza

    2012-01-01

    Full Text Available Luego de las grandes cambios que se dieron con la caída del Muro de Berlín y la desintegración en la URSS, se analizan, desde una perspectiva realista-sistémicaestructural, los componentes que intervienen en la generación del orden mundial, qué factores intervienen y cuáles son las diferentes aspiraciones respecto de las características que debería tener. Se tiene en especial consideración la creciente heterogeneización del sistema y la manera en que actores transnacionales operan como motores del futuro orden, predominando por sobre las posibilidades del actor históricamente dominante: el Estado-Nación. Se estudian las características principales del funcionamiento del sistema internacional y el significado de su marco institucional.

  12. Historicismo: notas sobre um paradigma

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    José D’Assunção Barros

    2012-08-01

    Full Text Available Este artigo tem por objetivo desenvolver algumas considerações sobre o Historicismo e sua possibilidade de ser examinado como um dos paradigmas que concorrem para o estabelecimento de uma história científica no século XIX. O primeiro ponto tratado é relativo às origens do paradigma Historicista na transição do século XVIII para o século XIX. Na sequência, aproximamo-nos da apropriação conservadora do paradigma historicista no novo contexto no qual a burguesia européia está assentada no Poder, combinando-se com a consolidação dos modernos estados-nações. A especificação das características deste paradigma constituem o principal ponto de análise

  13. Constitucional sobre la justicia ordinaria

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    José Luis Cea Egaña

    2005-01-01

    Full Text Available El artículo analiza el efecto vinculante y de irradiación de las sentencias del Tribunal Constitucional sobre el conjunto del ordenamiento jurídico y de sus órganos. El autor toma partido por la consideración de la ratio decidendi del fallo como vinculante junto al dictum de la sentencia respectiva. Se plantea la necesidad que la LOC del Tribunal Constitucional explicite los efectos vinculantes de los fallos respecto de los tribunales ordinarios y especiales. Finalmente analiza los efectos de la res iudicata en el ámbito del control reparador de constitucionalidad de preceptos legales en control concreto y en control abstracto.

  14. Puente sobre el rio Medway

    Directory of Open Access Journals (Sweden)

    Freeman, Fox, Ingenieros

    1963-02-01

    have been prestressed both longitudinally and transversally. Between the two circulation roadways an elastic barrier has been placed, supported on metal posts.El puente sobre el río Medway tiene la misión de salvar dicho río en el nuevo trazado de carretera necesario para aliviar el denso tráfico estival entre Dower y Londres. Está situado en las proximidades de la ciudad de Rochester. La estructura se ha construido utilizando las vías de concurso. De todos los tipos presentados se eligió una estructura de hormigón armado. La longitud total de la obra es de 1.000 m. Los tres tramos principales de la obra salvan el río. El central es de 152 m de luz, y los dos adyacentes son de 103 m cada uno. Los accesos tienen un total de 18 tramos, que arrojan una longitud de 654 m. La anchura del puente es de 34 m, y la calzada se ha subdividido en dos direcciones de tráfico de 7,30 m cada una y, además, se han dejado dos espaldones laterales de 2,40 m de anchura. En planta se puede admitir que el puente tiene una alineación recta con una ligerísima curva, y que en el plano vertical presenta una pendiente de desagüe entre las pilas 9 y 10. Los estribos son de hormigón armado: tipo celular el del este y de hormigón en masa el del oeste. Los estribos se apoyan sobre pilotes hexagonales de hormigón armado cuya circunferencia circunscrita tiene 55 cm de radio y de 12 a 20 m de longitud. Cuando el firme se hallaba a profundidades inferiores a 6 m, los estribos se cimentaban directamente sobre el firme. Las pilas constituyen pórtico y tienen una altura variable de 9 a 30 m sobre el nivel del suelo. Los tramos de acceso forman una estructura continua simplemente apoyada. Cada tramo se compone de ocho vigas longitudinales de 30 a 41 m de longitud y de 190 toneladas cada una. Estas vigas son del tipo pretensado y prefabricado. El tramo central es del tipo cantilever, constituido por dos brazos, de 30 m de longitud, formados por vigas cajón. Cada una de estas vigas consta

  15. Impuesto sobre el Valor Añadido. Apuntes

    OpenAIRE

    2013-01-01

    Apuntes sobre las siguientes cuestiones relacionadas con el Impuesto sobre el Valor Añadido: deducciones; exportaciones e importaciones; operaciones intracomunitarias; prorrata y sectores diferenciados.

  16. Puente sobre el Oosterschelde, Holanda

    Directory of Open Access Journals (Sweden)

    Hoving, H. T.

    1967-03-01

    Full Text Available This new bridge, forming part of the Delta project, is made of concrete, and was built by successive overhangs. Precast units were mainly used. The bridge is 5,000 m long, and is 11.85 m wide. The main spans are 95 m in length, except one, which can be opened up to sea traffic, which is 40 m long. The free height of the bridge over the sea is 15 m, which is insufficient for large ships. This required that one of the spans, in deep water, should open up, on hinges, and provide free passage to large ships. The intermediate bridge supports are of special interest. They rest on piles which go down to 38 m. They are hollow, cylindrical, of 4.35 m external diameter, and are driven into the ground in several stages, as they penetrate through the sands at the bottom of the Oosterschelde. To drive them in, an excavating device, inside the pile, was utilised, and also a dredging and suction mechanism, to clean out the bottom, and allow the piles to sink by gravity. But when external friction did not allow this to happen, the piles were weighed down with up to 500 tons of ballast. A series of three of these piles, in a row, makes up each bridge support. A cross member over the pile heads supports, in turn, the bridge deck. The bridge was built in successive sections, overhanging from each support, and then finally connected with a central portion. Use of precast units has been very extensive, so that the bridge can be justiaflably regarded as being prefabricated.Este nuevo puente, que forma parte del Plan de obras denominadas del Delta, es de hormigón y se ha construido por el procedimiento de voladizos sucesivos, utilizando, en su mayor parte, elementos prefabricados. Tiene una longitud total de 5.000 m; una anchura de 11,85 m; tramos principales de 95 m de luz, salvo uno, levadizo, que tiene 40 m. La altura libre sobre las ag^uas del mar es de 15 m, que es insuficiente para las naves de gran tonelaje; esto exigió que uno de sus tramos, en aguas

  17. PERSPECTIVAS INFANTILES SOBRE LA TERRITORIALIDAD

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    Noelia Enriz

    2009-12-01

    Full Text Available En este trabajo nos proponemos un acercamiento a las diversas ideas infantiles sobre el territorio habitado, en el contexto particular de la población mbyá guaraní de Misiones. La fragilidad territorialidad a la que es expuesta la población indígena en Argentina se manifiestan cotidianamente en diversas áreas. La población mbyá, ha sido surcada por los límites de los estados nacionales de Paraguay, Brasil y Argentina. La sedentarización y los cercamientos de tierras en los diferentes estados, han impulsado transformaciones de las lógicas de subsistencia, de habitación y organización comunitaria. En el caso de la provincia de Misiones la acreditación burocrática de los títulos de tierras se encuentra aplazada, el estado no se compromete con la concreción de este derecho.

  18. Sobre a psicopatologia dos atos

    Directory of Open Access Journals (Sweden)

    Roberto Calazans

    2015-07-01

    Full Text Available Este artigo pretende apresentar a psicopatologia dos atos a partir do esquema de Jacques Lacan sobre a angústia. Diferenciamos os acting-out, a passagem ao ato, os sintomas, as inibições e a angústia fazendo uma duplicação do esquema de Lacan levando em consideração os registros do simbólico, do real e do imaginário. Esse esquema permite uma abordagem da questão dos atos do sujeito sem cair em uma perplexidade do clínico, nem na confusão entre os atos e a ação. Permite também uma melhor orientação do clínico em relação às diversas modalidades de respostas subjetivas que não se restringem à questão sintomática, que é o campo inaugural da clínica psicanalítica desde a sua fundação por Sigmund Freud. Acreditamos contribuir, assim, para um maior esclarecimento desse esquema no ensino de Lacan e para que ele possa servir de orientação para o tratamento dos atos do sujeito na clínica.

  19. Puente colgante sobre el Lillebaelt

    Directory of Open Access Journals (Sweden)

    Ostenfeld, Chr.

    1971-09-01

    Full Text Available This bridge, which was opened to traffic in October 1970 over the Lillebaelt, is the first of its kind in Denmark. It consists of a central span of 600 m, and two lateral ones of 240 m length each. The suspension cables, each 1,500 m, are the world's largest prefabricated cables. The bridge has original features, such as flaps on the main box girder, and new design of the anchorage blocks. These and other details are described in the article.Inaugurado en octubre de 1970, el puente colgante sobre el Lillebselt es el primer puente danés de este tipo. Consta de un tramo central de 600 m y dos tramos laterales de 240 m. Los cables sustentantes son, con sus 1.500 m de longitud, los mayores cables prefabricados del mundo. El puente presenta características origínales —empleo de «flaps» en la vigacajón de la superestructura, concepción nueva de los macizos de anclaje...— que, junto con el sistema de ejecución y las demás particularidades de la obra, son descritas en el artículo.

  20. Estudo multicasos sobre atividades inovativas

    Directory of Open Access Journals (Sweden)

    Sonia Regina Hierro Parolin

    2013-09-01

    Full Text Available Em estudos sobre competitividade argumenta-se que as empresas necessitam de estratégias baseadas em inovações e de capacidades internas em constantes e intensas transformações, não somente nos padrões tecnológicos, mas também no encadeamento do processo de gestão das atividades inovativas, como processos dinâmicos, não lineares e diversificados, em função de múltiplos fatores dos ambientes interno e externo. Para contribuir com essas discussões, neste artigo apresenta-se um estudo multicasos em quatro indústrias de médio e grande portes, de segmentos, densidades tecnológicas e históricos com inovações diferentes entre si. Como principais resultados, salientam-se o encadeamento das atividades inovativas como parte de uma estratégia organizacional para obter resultados com inovação e a afluência de todas as pessoas, e não somente as alocadas em pesquisa e desenvolvimento, para o cumprimento dessa estratégia.

  1. Comentarios y reflexiones sobre las operaciones financieras

    OpenAIRE

    Pavón Bautista, María Mercedes

    1995-01-01

    En este trabajo se hacen comentarios y reflexiones sobre los tipos de operaciones financieras simples, operaciones financieras de constitución de capitales y operaciones financieras de amortización.

  2. Los derechos de autor sobre obtenciones vegetales

    Directory of Open Access Journals (Sweden)

    Rosario Silva Gilli

    2015-01-01

    Full Text Available Contenido: Las creaciones en la agricultura. Los derechos de autor sobre obtenciones vegetales. El Convenio Internacional para la protección de las obtenciones vegetales (París 1961 y sus revisiones de Ginebra 1972, 1978,1991 Objeto de la protección legal. Condiciones del cultivar. Alcance de la protección. La protección de obtenciones vegetales en el Mercosur. Los Derechos de autor en el Mercosur. El Protocolo de Armonización de normas sobre Propiedad Intelectual en el Mercosur. El Acuerdo de Cooperación y facilitación sobre la protección de las obtenciones vegetales en los Estados Partes del Mercosur.  Alcance de la normativa Mercosur. Los Derechos sobre cultivares en Uruguay. Los Derechos de autor como derechos humanos. ¿Derechos de autor vs. derecho de propiedad intelectual? Reflexión final

  3. Reflexiones sobre pedagogías intensivas

    Directory of Open Access Journals (Sweden)

    Vizcaíno Pinilla Mercedes

    1996-06-01

    Full Text Available

    En el presente artículo se pretende recoger los conceptos filosóficos y los principios pedagógicos que orientan las pedagogías intensivas. Como valor primordial se encuentra la acción comunicativa como el eje sobre el cual se construyen las pedagogías intensivas orienta sobre las calidades que debe tener el maestro para desarrollarlas

  4. PERSPECTIVAS INFANTILES SOBRE LA TERRITORIALIDAD

    Directory of Open Access Journals (Sweden)

    NOELIA ENRIZ

    2009-12-01

    Full Text Available In this paper we propose a different approach to children's ideas about the geographical area in the particular context of the Mbyá Guarani population, from Misiones (Argentine. The territorial fragility, that is exposed to the indigenous people in Argentina aremanifested daily in several areas. Mbyá population, has been crossed by the boundaries of national states of Paraguay, Brazil and Argentina. The sedentary and enclosures land processes in different states, have prompted changes in the logic of subsistence, housing and community organization. In Misiones province case, bureaucratic accreditation of land titles is postponed, the state is not committed to the realization of this right.RESUMEN: En este trabajo nos proponemos un acercamiento a las diversas ideas infantiles sobre el territorio habitado, en el contexto particular de la población mbyá guaraní de Misiones. La fragilidad territorialidad a la que es expuesta la población indígena en Argentina se manifiestan cotidianamente en diversas áreas. La población mbyá, ha sido surcada por los límites de los estados nacionales de Paraguay, Brasil y Argentina. La sedentarización y los cercamientos de tierras en los diferentes estados, han impulsado transformaciones de las lógicas de subsistencia, de habitación y organización comunitaria. En el caso de la provincia de Misiones la acreditación burocrática de los títulos de tierras se encuentra aplazada, el estado no se compromete con la concreción de este derecho.

  5. Consideraciones preliminares sobre la credibilidad

    Directory of Open Access Journals (Sweden)

    Fernando Verdugo

    2004-01-01

    Full Text Available A modo de introducción al Seminario Interno de Profesores de la Facultad de Teología de la PUC, el autor recuerda, en primer lugar, lo que suele entenderse por "credibilidad" y, también, algunos de los criterios generales por los cuales algo o alguien adquiere la calidad de creíble (el que sea razonable, relevante o significativo, capaz de producir gozo estético, etc.. Luego, en la parte más extensa, el autor vuelve a llamar la atención sobre la necesidad de atender a los condicionamientos culturales de la fe cristiana. La fe, al igual que toda experiencia humana, está culturalmente mediada. Tomar conciencia de ese hecho y asumir las consecuencias metodológicas es indispensable para una teología que quiere poner atención a los desafíos actuales y contribuir así a la credibilidad del cristianismoThis article reprints the introductory session to the internal seminar of lecturers from the Faculty of Theology of the UC. In the first place, the author sums up what is usually understood by `credibility' and the general criteria through which something or someone gains credibility (being reasonable, relevant or significant, capable of producing aesthetic pleasure. Then the author deals at length with the need to take notice of the cultural conditionings of the Christian faith. The faith, the same as any other human experience, is culturally mediated. It is crucial for theology to become aware of this fact and come to terms with its methodological consequences, if it wants to face current challenges and so contribute to the credibility of Christianity

  6. Sobre sujeitos racionais e apaixonados

    Directory of Open Access Journals (Sweden)

    Dílson Ferreira da Cruz

    2009-12-01

    Full Text Available

    Desde seu nascimento, a Retórica convive com um cisma insuperável: de um lado, Platão afirma que o orador deve buscar o bom e o verdadeiro; tem de seguir a lógica e sustentar-se com um discurso sóbrio, no qual prevalece a razão, o logos, e para o qual as coisas interessam pelo que são em si mesmas. No outro extremo da ágora está Cícero, para quem a Retórica não tem que procurar pela essência de nada, mas fazer com que a opinião do orador, seja ela verdadeira ou não, passe a ser também a do auditório. Para tanto, a lógica, tão acalentada por Platão, é de bem pouca valia, pois o orador, se quiser ter sucesso, deve levar os corações da cólera à doçura ou desta àquela. Como se vê, é uma retórica alicerçada sobre o pathos, ou, como diriam os semioticistas, no estado das almas. Este artigo se propõe a pensar essa questão utilizando o instrumental da semiótica dita tensiva com o objetivo de mostrar que, no discurso do grego, predominam as valências ligadas à extensidade, ao inteligível, às características dos objetos; ao passo que, nos ensinamentos do romano, são as valências relacionadas à intensidade, ao sensível, que se destacam.

  7. A foodborne outbreak of Cryptosporidium hominis infection

    DEFF Research Database (Denmark)

    Ethelberg, S.; Lisby, M.; Vestergaard, L. S.;

    2009-01-01

    separate salad bar ingredients were found to be likely sources: peeled whole carrots served in a bowl of water, grated carrots, and red peppers (in multivariate analysis, whole carrots: OR 2.1, 95% CI 1.1-4.0; grated carrots: OR 2.1, 95% CI 1.2-3.9; peppers: OR 3.3, 95% CI 1.7-6.6). We speculate...

  8. Impacto psicosocial del Downsizing sobre los trabajadores

    Directory of Open Access Journals (Sweden)

    CRISTÓBAL JIMÉNEZ JIMÉNEZ

    2006-01-01

    Full Text Available El presente trabajo intenta ahondar en lo expuesto en un trabajo anterior sobre el downsizing como fenómeno psicosociolaboral, analizando su impacto psicosocial. En primer lugar, se exponen los diferentes niveles de comprensión de este fenómeno, para pasar al análisis de los efectos y consecuencias sobre las personas, diferenciando entre dos tipos de víctimas: a los que se van de la organización (despedidos y b los que se quedan en la organización (supervivientes, prestando especial atención a lo que se conoce ya como el "síndrome del superviviente", para lo que se aporta información sobre sus características, las variables moderadoras que intervienen y los modelos explicativos del mismo. Se termina con algunas consideraciones sobre la actualidad y relevancia de dicho síndrome sugiriendo la idea de que el fenómeno del downsizing y sus consecuencias sobre las personas nos enfrenta a lo que podríamos ya considerar como un nuevo síndrome de la Psicopatología Laboral: el síndrome del superviviente.

  9. DISERTACIÓN SOBRE JUSTICIA Y EQUIDAD

    Directory of Open Access Journals (Sweden)

    Sayuri P. Tamura M.

    2001-01-01

    Full Text Available «Disertación sobre equidad y justicia» es básicamente una respuesta a una inquietud en particular que yo tenía sobre las distintas posiciones que existen sobre estos dos temas y cómo definir cuál es la más acertada. Hice una descripción del punto de vista de Amartya Sen –la violación de los derechos sobre la propiedad privada es justificable si evitan las hambrunas2– frente al de Robert Nozick –la propiedad privada es un derecho fundamental y como tal debe ser respetado3–, sin embargo, aunque ambos enfoques son totalmente contrarios tienen mucho sentido, por lo cual es bastante difícil decidirse por uno en particular. Fue de esta forma, enfrentando sus opiniones, que yo logré desarrollar una posición frente a estas dos perspectivas, y así dar mi opinión al DISERTACIÓN SOBRE JUSTICIA Y EQUIDAD SAYURI P. TAMURA M. Estudiante de Economía y Negocios Internacionales de la Universidad ICESI. respecto. Al final, llegué a una conclusión, que aunque no es la más original y tampoco es la única, tiene mucho sentido y seguramente de ser posible, la situación del país podría cambiar para bien.

  10. Sobre la sublimación

    OpenAIRE

    2007-01-01

    Este documento presenta un trabajo de investigación realizado sobre un concepto clave para la producción teórica y la enseñanza en psicología, como es la noción de sublimación. La investigación indaga la sublimación en el marco de la teoría psicoanalítica, con el objeto de profundizar su explicación conceptual y decidir sobre la pertinencia de tomarlo como noción clave para explicar la producción social del saber.

  11. Principios sobre semáforos

    OpenAIRE

    Valencia Alaix, Víctor Gabriel

    2000-01-01

    Debido a la ausencia de una publicación que reuniese los aspectos más relevantes sobre semáforos y en el marco académico sobre ingeniería de tránsito de los cursos de pregrado y posgrado en Vías y Transporte de la Universidad Nacional de Colombia - Sede Medellín, se ha preparado este documento como guía introductoria a dicho tema. En su preparación se han recogido los tópicos principales de varias publicaciones internacionales y nacionales, además, su desarrollo ha considerado la experi...

  12. Debate sobre estudios postcoloniales y subalternos

    OpenAIRE

    David Morris; McLean, Ian S.; Hillel Eyal; Ivette Hernández; Richard W. Slata; Betsy Campisi; Jackson, Robert H; William Patterson; Kathryn Lehman; Chad Black; Michelle Molina; Ann Twinam; Dennis Hidalgo

    2001-01-01

    A partir de los comentarios a la reseña elaborada por Ian S. Mclean sobre el libro: Cuerpos incas y el cuerpo de Cristo. El Corpus Christi en Cuzco colonial, Perú, escrito por Carolyn Dean, surge un debate sobre los Estudios Postcoloniales y Subalternos, que se desarrolló entre el 12 de agosto y el 3 de septiembre de 2001, a través de la lista de correo electrónico de H-Latam, grupo de discusión dedicado a la historia latinoamericana. En él participaron destacados especial...

  13. Jose carlos mariategui: sus articulos sobre arte

    OpenAIRE

    Henríquez, Cecilia

    2014-01-01

    En la prolífica obra que colma la corta vida del pensador latinoamericano,se encuentran profundas reflexiones sobre el arte que valela pena recordar en e1 año del centenario de su nacimiento. Los juiciosque propone sobre el arte están n1ediados por su pensamiento marxista.Aunque gran conocedor del arte de sus contemporáneos, sucompromiso socialista, cada vez mayor, reduce el espacio del análisisestilístico.Un punto de partida para observar el pensamiento estético deJosé Carlos Mariátegui es s...

  14. Impacto psicosocial del Downsizing sobre los trabajadores

    OpenAIRE

    CRISTÓBAL JIMÉNEZ JIMÉNEZ; JOSÉ LUIS SÁNCHEZ LAGUNA

    2006-01-01

    El presente trabajo intenta ahondar en lo expuesto en un trabajo anterior sobre el downsizing como fenómeno psicosociolaboral, analizando su impacto psicosocial. En primer lugar, se exponen los diferentes niveles de comprensión de este fenómeno, para pasar al análisis de los efectos y consecuencias sobre las personas, diferenciando entre dos tipos de víctimas: a) los que se van de la organización (despedidos) y b) los que se quedan en la organización (supervivientes), prestando especial atenc...

  15. Consideraciones sobre los ensayos de laboratorio

    Directory of Open Access Journals (Sweden)

    José Gabriel Gómez Cortés

    2013-12-01

    Full Text Available Texto de la conferencia dictada por el autor en el Instituto de la Construcción y del Cemento en Madrid. Eduardo Torroja, España, como asistente al IX Curso de Estudios Mayores de la Construcción sobre "LA EDIFICACION Y SU PATOLOGÍA". Se analizan aspectos sobre: los criterios para solicitar ensayos, muestreo, variabilidad de resultados y reproducibilidad de ensayos, criterios de aceptación e importancia de la retroalimentación.

  16. Generalidades sobre el 68 en la narrativa

    OpenAIRE

    Ocampo, Aurora M.

    2013-01-01

    Es mucho lo que se ha escrito sobre el movimiento estudiantil de 1968, según mis registros, cerca de un centenar de libros, además de innumerables artículos, ensayos, narraciones, poemas, teatro y reseñas a los libros como a los grandes ensayos, aparecidos en publicaciones periódicas. Por otro lado, muchos de los libros, ya sean testimoniales, de reflexión sobre lo sucedido, antologías de poesías, ensayos, cuentos, teatro y narrativa en general se han reeditado varias veces; ahora, con motivo...

  17. Enteroparásitos: detección y vigilancia en aguas residuales de Costa Rica durante 1999

    Directory of Open Access Journals (Sweden)

    Mariano Peinador

    2000-12-01

    Full Text Available Durante el año 1999 se realizó un monitoreo de los cinco sistemas lagunares de tratamiento de aguas residuales domésticas y de una planta de Iodos activados que posee el Instituto Costarricense de Acueductos y Alcantarillados en Costa Rica. Este monitoreo consistió en toma y análisis de muestras bimensuales en el agua afluente y efluente de dichos sistemas de tratamiento. El objetivo de este trabajo es estudiar la eficiencia en la remoción de huevecillos de helmintos y quistes de protozoarios patógenos que tienen los diferentes sistemas de tratamiento y comparar los resultados con la epidemiología de las zonas correspondientes, al respecto. Las muestras se analizaron por el método de Ritchie modificado. Se encontró una baja eficiencia en la remoción de estos organismos por parte de la planta de Iodos activados, en lo que respecta a Entomoeba histolytica, E. Coli,Blastocystis hominis y Lamblia intestinalis, y una eficiencia del 100% en todos los sistemas lagunares. Estos resultados concuerdan con los datos sobre parasitosis por enteropatógenos, obtenidos de los diferentes hospitales y clínicas de las zonas en estudio.

  18. Puente pretensado sobre el Guadarrama. Villalba

    Directory of Open Access Journals (Sweden)

    Páez, A.

    1958-12-01

    Full Text Available En este artículo se describe y comenta la solución dada al puente de hormigón pretensado sobre el río Guadarrama, primero de los construidos mediante dovelas prefabricadas y solidarizadas después, mediante el pretensado de la armadura longitudinal.

  19. Un nuevo libro sobre Juan de Castellanos

    Directory of Open Access Journals (Sweden)

    Alberto Miramón

    1979-02-01

    Full Text Available El Centro de Historia del Táchira ha auspiciado la publicación, por la Editorial Kelly de Bogotá, del segundo libro que monseñor Mario Germán Romero ha escrito sobre la vida y obra, tan apasionante ambas, de don Joan de Castellanos

  20. O pensamento de Marx sobre a subjetividade

    Directory of Open Access Journals (Sweden)

    Eduardo F. Chagas

    2013-08-01

    Full Text Available O pensamento de Karl Marx sobre a subjetividade humana é pouco conhecido e divulgado na língua portuguesa, e, no Brasil, particularmente, carece ainda de um estudo amplo, explícito e sistemático. Meu artigo pretende esboçar uma reflexão mais completa de sua filosofia sobre a subjetividade humana, insistindo não somente na crítica, mas também, e especialmente, na compreensão da referida questão, a partir de uma leitura imanente e estrutural de suas obras, no original. Vale ainda ressaltar que minha investigação se apoia na conexão entre subjetividade e objetividade, entre sujeito e objeto, inquirindo se há um determinismo da objetividade sobre a subjetividade, ou seja, se essas duas determinações são contraditórias no interior do pensamento marxiano, comprometendo, pois, as suas reflexões acerca da crítica à filosofia especulativa de Hegel e ao empirismo da economia clássica, ou se, na verdade, tal conexão é o segredo recôndito de sua filosofia sobre a subjetividade humana.

  1. Los derechos reales sobre cosa ajena

    OpenAIRE

    Panero Oria, Patricia

    2011-01-01

    Esta presentación cierra la serie de presentaciones que sa han realizado sobre los Derechos reales, según el Plan Docente seguido en la Facultad de Derecho de la Universidad de Barcelona, y que sirven de guía para el estudio de la materia.

  2. Las fuentes cristianas sobre Jesús

    Directory of Open Access Journals (Sweden)

    Melero Martínez, J.M.

    2010-01-01

    Full Text Available El artículo es una presentación de las fuentes históricas sobre Jesús de Nazaret: fuentes sinópticas, fuente Q, fuentes afines a la gnosis, fragmentos de evangelio con material sinóptico o joánico, evangelios judeocristianos y otras fuentes.

  3. Observaciones sobre didáctica

    Directory of Open Access Journals (Sweden)

    Alfonso Tamayo Valencia

    2014-02-01

    Full Text Available  En el presente artículo, a partir de la conceptualización sobre didáctica y enseñanza, se plantean sus posibilidades en la educación superior, especialmente cuando reflexionamos en las prácticas de enseñanza desde un saber hacer que supere el hacer de esquemas tácitos y espontáneos que caracteriza en buena parte docencia universitaria. Desde esta perspectiva se aboga por la renovación de la concepción de la didáctica desde una postura epistemológica y psicológica sobre la manera como se construye el conocimiento.

  4. Sobre lectura y escritura en Chile

    Directory of Open Access Journals (Sweden)

    Grínor Rojo

    2011-11-01

    Full Text Available Los estudios de los últimos años realizados en Chile sobre lectura, competencia de lectura y lectura de noticias sobre política, revelan valores negativos que rondan el 50. Estos datos, a los que se suman los cuarenta millones de analfabetos en América Latina, están en la base del rechazo en este artículo de la afirmación de la muerte del libro y de la frívola fe en el reemplazo del libro por el uso de las tecnologías de la información y la comunicación (llenar de computadoras las escuelas, insistiendo en cambio en atender seriamente a los lazos entre razón, libro y lectura en el desarrollo individual y de la sociedad

  5. Representaciones sociales juveniles sobre la democracia

    Directory of Open Access Journals (Sweden)

    Álvaro Díaz Gómez

    2005-01-01

    Full Text Available El presente artículo da cuenta de una parte de los resultados obtenidos en la investigación “representaciones sociales juveniles sobre la democracia y la multiculturalidad”. Se desarrolló desde la perspectiva metodológica de la Investigación Acción Participante, proceso dentro del cual, se aplicó un cuestionario conformado por nueve preguntas, divididas en tres bloques temáticos que permitieron conocer las representaciones sociales de un grupo de veinticinco jóvenes, sobre la democracia –aspecto que se reporta en este texto– la ciudadanía y la multiculturalidad a partir de tres dimensiones: significado, vivencias y condiciones de la democracia.

  6. Sobre lectura y escritura en Chile

    OpenAIRE

    Grínor Rojo

    2011-01-01

    Los estudios de los últimos años realizados en Chile sobre lectura, competencia de lectura y lectura de noticias sobre política, revelan valores negativos que rondan el 50. Estos datos, a los que se suman los cuarenta millones de analfabetos en América Latina, están en la base del rechazo en este artículo de la afirmación de la muerte del libro y de la frívola fe en el reemplazo del libro por el uso de las tecnologías de la información y la comunicación (llenar de computadoras las escuelas), ...

  7. Radiaciones ionizantes y efectos sobre la materia

    Directory of Open Access Journals (Sweden)

    Sandra Magaly Medina Araujo

    2002-01-01

    Full Text Available Las radiaciones ionizantes, además, de tener muchas aplicaciones benéficas tanto en el campo industrial como en los organismos vivientes, presentan también muchos efectos nocivos que se deben evitar, sobre todo cuando se trata de fuentes artificiales con las que pueden presentarse abusos en su utilización, especialmente en la parte clínica. Este artículo, el primero de una serie de divulgaciones acerca del tema, pretende dar a conocer las características y efectos sobre la materia, de las mencionadas radiaciones, así como algunas recomendaciones acerca del manejo y tralAS, tamiento de dichas radiaciones

  8. Prevalence of Cryptosporidium spp. and other intestinal parasites in children with diarrhea

    Directory of Open Access Journals (Sweden)

    Mutalip Çiçek

    2011-03-01

    Full Text Available This study was planned to determine the role of Cryptosporidium sp. and other intestinal parasites in the diarrheal diseases in children with 0-15 years old Van district.Materials and methods: In this study, stool samples of 450 children were examined for parasites. In the study, nativ-lugol, formaldehyde-ethyl acetate sedimentation methods and trichrome staining methods were used to detect parasites in stool samples. Additionally, sedimentation methods and modified acid fast staining method were used to detect the Cryptosporidium oocysts.Results: Parasites were found in 154 (34.2% among 450 children’s with diarrhea. In this study; the ratios of parasites were as follow: Giardia intestinalis 13.5%, Blastocystis hominis 10%, Entamoeba coli 3.78%, Cryptosporidium spp. 2.2%, Hymenolepis nana 1.33 %ve Ascaris lumbricoides 1.11%.Entamoeba histolytica/Entamoeba dispar 0.89%, Chilomastix mesnili 1.78%, Iodamoeba butschlii 0.89%, Entamoeba hartmanni 0.89%, Trichomonas hominis 0.67%, Enteromonas hominis 0.67%,Conclusion: In the investigate, it was found that Giardia intestinalis and Blastocystis hominis were most prominent agents in children with diarrhea in our vicinity and Cryptosporidium spp also was an important agent which should be investigated carefully in especially risk group in routine laboratory studies.

  9. Monografía FOMIN sobre Remesas

    OpenAIRE

    Inter-American Development Bank; Analistas Financieros Internacionales (AFI)

    2009-01-01

    Consultoria realizada por Analistas Financieros Internacionales para Fondo Multilateral de Inversiones sobre: Flujos de remesas a América Latina: Características del mercado de remesas y sus actores principales. Aspectos regulatorios relevantes para facilitar el rol de las remesas como instrumento de desarrollo. Descripción de la cartera de proyectos FOMIN y su nueva estrategia en el Cluster de Remesas.

  10. Cumbre Mundial sobre Desarrollo Social. Copenhague

    OpenAIRE

    Abellán García, Antonio

    1995-01-01

    La Asamblea General de Naciones Unidas adoptó en 1992 la decisión De convocar una Cumbre Mundial sobre Desarrollo Social para afrontar los problemas de pobreza, desempleo e integración social. La Cumbre tuvo lugar en marzo de 1995, en Copenhague, y se desarrolló en tres partes: Plenario, Comité Principal y Cumbre de Jefes de Estado o Gobierno

  11. Reflexiones sobre el concepto de justicia

    Directory of Open Access Journals (Sweden)

    Víctor C. Pascual Planchuelo

    2005-06-01

    Full Text Available Este ensayo constituye una reflexión sobre el concepto de Justicia. No se centra, como tantos estudios al respecto, en el plano teórico y metafísico, sino que atiende problemas de mayor actualidad: la opinión de los ciudadanos, la postura de los profesionales y otros asuntos controvertidos del ámbito del Derecho.

  12. Sobre la felicidad y la ataraxia

    Directory of Open Access Journals (Sweden)

    Alberto Londoño Alvarez

    1969-09-01

    Full Text Available Muy pocos lectores, seguramente, no han tomado en momentos de crisis nerviosas, de desarreglos emocionales, de intranquilidad y angustia, alguna de las tantas drogas que se conocen con el mote genérico de "ataráxicos", de "tranquilizantes". Pues bien, hablemos un poco sobre este tema, sus antecedentes históricos y filosóficos y lo que para los griegos clásicos significaba el vocablo "ataraxia".

  13. Drug sensitivity of ureaplasma unrealytium accompanying mycoplasma hominis infection in female genital tract and its therapeutic effect%女性生殖道解脲支原体合并人型支原体感染药敏分析及疗效观察

    Institute of Scientific and Technical Information of China (English)

    郭树琴; 王振国; 宋萍

    2011-01-01

    目的 评价联合用药与单独用药治疗女性生殖道感染的疗效.方法 将2009年10月-2010年10月我院妇科门诊就诊76例生殖道解脲支原体(Ureaplasma Urealyticum)合并人型支原体(Mycoplasma Hominis)感染患者随机分成2组.联合用药组:外阴阴道冲洗(0.9%氯化钠注射液稀释5%碘伏1:10),口服强力霉素每次4mg,1次/d,连服7d,同时干扰素栓剂置阴道深部,隔日1次,共14d.单独用药组:口服强力霉素每次4mg,1次/d,共7d.停药1周后行妇科检查,停药2周后做官颈支原体培养.结果 76例患者对强力霉素、交沙霉素、美满霉素较敏感,敏感率依次为61.9%、59.2%和50%,而对罗红霉素、螺旋霉素、克拉霉素高度耐药,耐药率依次为90.8%、88.2%和76.3%.联合用药组总有效率92.1%,单独用药组总有效率78.9%,有显著差异(P<0.001).结论 应用外用干扰素栓剂联合强力霉素比单独口服强力霉素治疗女性生殖道解脲支原体合并人型支原体感染效果好.%To evaluate the therapeutic effect of combined doxycycline and interferon suppository or doxycycline alone on female genital tract infection. Methods Seventy-six patients infected with ureaplasma unrealytium and mycoplasma hominis who visited our department from October 2009 to October 2010 were randomly divided into combined drug treatment group(group A) and single drug treatment group(group B). Vulvovagina of patients in group A was irrigated with 0.9% normal saline and 5% iodophor(l:10). Then, the patients were treated with doxycycline(4mg), once a day for 7 days. Interferon suppository was placed in the deep vagina, every other day for 14 days. Patients in group B were treated with oral doxycycline only(4mg), once a day for 7 days. One week after withdrawal of the drugs, the patients in two groups underwent gynecological examination. Two weeks after withdrawal of the drugs, mycoplasma from cervix was cultured. Results The sensitivity rate of the 76 patients to

  14. ESTUDO SOBRE O IMPACTO DOS PROCESSADORES HOSPEDEIROS

    Directory of Open Access Journals (Sweden)

    Alba S. B. Lopes

    2008-05-01

    Full Text Available Arquiteturas reconfiguráveis são dispositivos de hardware capazes de modificar sua estrutura de hardware de acordo com a aplicação a ser executada. Tais dispositivos surgiram como uma alternativa às soluções de hardware tradicionais na tentativa de equilibrar flexibilidade e desempenho. Uma das principais formas de utilização de arquiteturas reconfiguráveis corresponde a um bloco reconfigurável anexado a um processador hospedeiro. Esse processador é responsável por executar diversas tarefas críticas, incluindo o particionamento hardware/software e indicar o momento do bloco reconfigurável executar. Apesar de existirem diversas propostas de arquiteturas reconfiguráveis na literatura, pouco se estudou sobre o impacto do processador hospedeiro na arquitetura. Nesse contexto, esse artigo apresenta um estudo sobre o impacto do processador hospedeiro no desempenho da arquitetura reconfigurável híbrida. Como estudo de caso foi realizada uma avaliação sobre o uso dos processadores SPARC V8 da Sun Microsystems e Nios II da Altera como processadores hospedeiros da arquitetura reconfigurável híbrida RoSA. O objetivo deste estudo é realizar a comparação entre o desempenho desses processadores e identificar qual deles é o mais adequado para anexar a arquitetura em questão. Os resultados mostraram que foi possível alcançar até 47% de ganho de desempenho com o uso do processador Nios II em comparação ao SPARC V8, indicando o primeiro como mais adequado para a arquitetura RoSA. PALAVRAS-CHAVE: arquiteturas reconfiguráveis, processador hospedeiro, desempenho.

  15. Algunas consideraciones sobre la arquitectura maya

    OpenAIRE

    Cejudo Collera, Mónica; DEPFA UNAM

    2013-01-01

    Con la finalidad de definir las variantes regionales y estilísticas que utilizaron los cons- tructores de la sociedad maya a lo largo de las varias etapas de su desarrollo, se estu- dian, en este artículo, los elementos que conforman las estructuras de la arquitectura maya. El resultado de este proceso de análisis permite ver cómo, a partir de una con- cepción global de la arquitectura y el urbanismo mayas, cada sitio se desarrolló sobre la base de su singularidad.

  16. Debate sobre el agua : punto de partida

    OpenAIRE

    2013-01-01

    A lo largo de este número de la revista encontrarás artículos concretos sobre el uso del agua en la agricultura, las bondades e inconvenientes de diferentes sistemas agrícolas y advertencias a los riesgos de privatización del agua, entre otros. Son temas que, desde la perspectiva de la Soberanía Alimentaria, debemos abordar contestando una pregunta fundamental: para que las y los agricultores puedan alimentar al Planeta y mantenerlo fértil, ¿qué uso y gestión hacemos del Agua?

  17. II Jornadas sobre Emprendimiento Social y Colectivo

    OpenAIRE

    Centro de Investigación en Gestión de Empresas

    2013-01-01

    La línea temática principal del encuentro es el emprendimiento social y colectivo así como las diferentes líneas de investigación de los institutos y centros de la Red relacionadas, como: responsabilidad social, empleo y reincorporación al trabajo, inserción laboral, servicios sociales y dependencia, género, cambios legislativos, utilidad social, medio ambiente, desarrollo local,... Centro de Investigación en Gestión de Empresas (2013). II Jornadas sobre Emprendimiento Social y Colectivo. ...

  18. Modelos mentales sobre el concepto de medida

    OpenAIRE

    Osorio, Andrea Milena

    2009-01-01

    Esta tesis de maestría presenta los resultados de una investigación que tuvo como pretensión comprender la estructura de los modelos mentales sobre el concepto de medida que poseen estudiantes de segundo grado de la educación básica y los estudiantes de formación complementaria (estudiantes que inician la formación docente). Para recoger la información, se emplearon técnicas de recolección de información, la observación y la entrevista semiestructurada, que fueron aplicadas a 8 estudiant...

  19. Modelos mentales sobre el concepto de medida

    OpenAIRE

    Osorio, Andrea Milena; García, Ligia Inés

    2011-01-01

    El objetivo de esta investigación ha sido comprender la estructura de los modelos mentales sobre el concepto de medida que caracteriza a un grupo de estudiantes de segundo grado de básica primaria y de primer año de formación complementaria. En efecto, el interés es identificar los elementos que se dan en los modelos y analizar las posibles relaciones entre dichos elementos. El informe de investigación se encuentra presentado en 7 apartes. La primera parte comprendida por los numerales ...

  20. Apuntes sobre el cultivo del cacao

    OpenAIRE

    Chavarriaga, Eduardo; Ochoa, Horacio

    2013-01-01

    El sombrío constituye uno de los problemas que se presentan en el cultivo del cacao, de necesaria resolución para ayuda del agricultor en su labor al producir tan importante grano. Contribuye a la economía del cultivo un conocimiento amplio de la cuestión que debe preocupar al productor inquiriendo no solamente por lo acostumbrado en la región, sino por lo que en otras partes se conoce sobre el particular, para abandonar un poco el emperismo y hacer una industria cada día más próspera. Co...

  1. Conceptos fundamentales sobre el mantenimiento de edificios

    OpenAIRE

    Juan Miguel Arencibia Fernández

    2007-01-01

    En esta investigación se realiza una profunda búsqueda sobre el tema del Mantenimiento de Edificios donde se abundan diferentes conceptos y criterios de la misma, encontrándose de esta forma los más utilizados métodos a emplear. Luego teniendo en cuenta las clasificación del Mantenimiento de Edificio según los tipos de obras, propietarios del inmueble y el momento que éste se realiza, como los costos de una edificación y su equipamiento a utilizar. Otros de los aspectos a tener en cuenta es l...

  2. Ensayo sobre la melancolía

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    Marta Chacón Salinas

    2013-07-01

    Full Text Available Este trabajo es un intento de delimitar la melancolía del conjunto de los trastornos depresivos y de reflexionar sobre sus características tal como aparecen en el discurso de los melancólicos. Se consideran algunas hipótesis en la línea psicoanalítica freudiana y lacaniana (Lambottc, Kristeva para entender cómo se va constituyendo en sus características principales: la intolerancia a la pérdida del objeto y el desfallecimiento del símbolo que le impide elaborar este duelo.

  3. Sobre Wittgenstein y la ética

    OpenAIRE

    García Soto, Luis

    2010-01-01

    El objeto de este trabajo es analizar y discutir la visión que de la ética da Wittgenstein en su “Conferencia sobre Ética” (1930). En nuestra opinión, la visión de la ética dada por el filósofo está más próxima de la religión o de la literatura que de otros sistemas normativos, como el derecho, la política e incluso la economía. De acuerdo con esto, intentamos ofrecer otra visión de la ética.

  4. Literatura infantil sobre problemas infantiles: la diabetes

    OpenAIRE

    Lázaro Fernández, Patricia

    2015-01-01

    Debido al creciente número de casos de diabetes infantil que se está dando en la sociedad actual, no es raro encontrarse en las aulas cada vez más casos de esta enfermedad. Por esta razón, he decidido analizar dos libros sobre esta temática, para hacer un acercamiento del material seleccionado a las aulas y poder desarrollar junto con los niños un aprendizaje colectivo y cooperativo. En este trabajo se expone la importancia de la literatura y la literatura infantil como una ...

  5. Comentarios sobre dibujo analítico

    OpenAIRE

    Mata Botella, Elena

    2004-01-01

    Cuaderno de apoyo a la docencia del DIBUJO DE ARQUITECTURA que pretende hacer reflexionar al alumno (de primero o segundo de carrera) sobre un tipo de dibujo que aquí se ha llamado “dibujo analítico”. Un dibujo que a través de operaciones gráficas y conceptuales como la esquematización y la selección de información, tiende a alejarse de la descripción del objeto arquitectónico para adentrarse en determinados aspectos o dimensiones que subyacen en el proyecto arquitectónico. Unas notas intr...

  6. Nuevas tecnologias: sobre computadores y brujas

    OpenAIRE

    Buenaventura, Juan Guillermo

    1987-01-01

    No soy ingeniero, ni experto en nuevas tecnologías. Cualquier comentario que haga no puede ser más que o bien el comentario de un ciudadano preocupado por su país o bien el acercamiento personal al tema, por parte de un comunicador interesado en los impactos y potencialidades, que tienen estas tecnologías en el ejercicio profesional y en nuestra cultura. A mi modo de ver la literatura conocida sobre las nuevas tecnologías oscila entre dos polos, dentro de los cuales se encuentran los diferent...

  7. Efecto del Diazepam sobre el desarrollo fetal

    OpenAIRE

    2001-01-01

    El diazepam se absorbe rápida y completamente después de la administración oral y atraviesa la barrera placentaria; su tasa de penetración en el tejido placentario es de 31,5% a las 4 horas, su vida media es prolongada y la existencia de metabolitos activos hace que su efecto sea acumulativo. Este trabajo se realizó en un modelo in vivo, usando 10 mg/Kg/día de diazepam intramuscular, en donde se estudió el efecto del diazepam sobre el desarrollo fetal, utilizando un modelo experimental en el ...

  8. Perspectivas sobre la comunicación

    OpenAIRE

    Naranjo Pereira, María Luisa

    2011-01-01

    Existen diversas teorías, enfoques y modelos de comunicación; de cada uno de ellos pueden rescatarse aspectos de gran valor para el logro de una comunicación eficaz. Este documento presenta una revisión de esos aspectos, para que se pueda comprender mejor y enriquecer uno de los procesos más importantes para el ser humano: el proceso de comunicación. Este es un ensayo en el cual se da a conocer la posición de la autora sobre el tema abordado. Se rescata el acento del enfoque humanista en la ...

  9. Apuntes Sobre el Cultivo del Cacao.

    Directory of Open Access Journals (Sweden)

    Ochoa Horacio Ochoa

    1940-12-01

    Full Text Available En el presente artículo se hablara del proceso de fermentación del cacao, pero antes de entrar a detallar este proceso, es conveniente dar algunas anotaciones sobre los sistemas de recolección o “cosecha” empleados en distintos lugares, pues ellos tienen sus diferencias y en ocasiones no se efectúan con todo el cuidado necesario tanto para el árbol como para el producto que irá a los depósitos de fermentación.

  10. Soportes apoyados sobre vigas pretensadas (EE. UU.

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    Editorial, Equipo

    1965-12-01

    Full Text Available This work has been awarded a prize by the Prestreesed Concrete Institute. It refers to a number of flyovers on the Arroyo Seco, of California. This river has been canalized, to help drainage in case of heavy rainfall. The canal runs through a highly populated zone, and a motor road and accessory ramps had to cross the canal. The resulting design involves three prestressed girders, lightly arched in a parabolic outline, resting on each river shore, running along the line of the bridge, over the canal. It was decided that the bridge piles should not rest on the river bed, in order not to reduce its flow capacity. The girders of a maximum length of 39 ms, are box shaped, and stiffened with thin diaphragms. The cylindrical pile, 1.50 m in diameter, is an integral part of the girder, and applies to it a concentrated load of about 4000 tons. The girders rest on reinforced concrete cubes, which in turn are supported by concrete slabs and piles driven into the soil.Esta obra ha sido premiada por el Prestressed Concrete Institute y se refiere a una serie de pasos superiores sobre el Arroyo Seco, en California, que se ha canalizado para servir de desagüe en el caso de avenidas torrenciales. El canal se halla en una zona de edificación densa y se trata del paso—sobre aquél—de una autopista y rampas de acceso. La solución adoptada consistió en tres vigas pretensadas, ligeramente arqueadas con perfil parabólico, apoyadas en las dos márgenes y situadas en correspondencia con los tres soportes, sobre el propio canal, ya que ninguno de aquéllos debía apoyarse en el lecho de este último al objeto de no disminuir su capacidad de desagüe. Las vigas—de unos 39 m de longitud la mayor—son del tipo cajón, rigidizadas con unos diafragmas de pequeño espesor. El soporte cilíndrico, de 1,50 m de diámetro, se solidariza con la viga y le transmite una notable carga concentrada, del orden de 4.000 toneladas. Los apoyos de estas vigas son simples dados

  11. Efectos del tabaquismo sobre el desarrollo prenatal

    OpenAIRE

    Carnero Cartujo, Sonia

    2014-01-01

    El tabaquismo es un problema de salud pública, causa enfermedad, incapacidad y muerte. El porcentaje de mujeres fumadoras mundialmente, alcanza el 22%. Si este porcentaje se mantiene durante el embarazo, debería tenerse en cuenta que la placenta humana es particularmente permeable a muchos de los compuestos en el humo del tabaco, y que existe suficiente evidencia científica de que los componentes del humo de un cigarrillo tienen efectos muy negativos sobre la vida intrauterina. La mayor parte...

  12. Tributos sobre a folha ou sobre o faturamento? Efeitos quantitativos para o Brasil

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    Tiago V Cavalcanti

    2008-09-01

    Full Text Available Este artigo estuda os impactos alocativos e de bem-estar de uma reforma tributária que substitui os impostos sobre a folha de salários por outro que incide sobre a receita. Para isso, desenvolve-se um modelo neoclássico de crescimento econômico com impostos sobre a receita e sobre a folha de salários. As simulações com parâmetros da economia brasileira mostram que tal reforma tributária tem um efeito negativo no bem-estar, no estoque de capital de longo prazo e consequentemente no produto per capita de longo prazo. Contudo, o emprego aumenta.This paper studies the impacts of a tax reform which substitutes taxes on labor income by taxes on total revenue. In order to accomplish that, we construct and solve a general equilibrium neoclassical growth model with taxes on labor income and total revenue. The numerical simulations with Brazilian parameters show that such tax reforms would negatively affect welfare, the capital stock, and consequently per capita output. However, employment would increase. Such effects are quantitatively important.

  13. Nuevas operaciones binarias sobre los conjuntos borrosos de tipo 2

    OpenAIRE

    Hernández Varela, Pablo Ramón; Cubillo Villanueva, Susana; Torres Blanc, Carmen

    2013-01-01

    Walker et al. ([19], [20]) definieron dos familias de operaciones binarias sobre M (conjunto de las funciones de [0,1] en [0,1]), y determinaron que, bajo ciertas condiciones, estas operaciones son tnormas (normas triangulares) o t-conormas sobre L (subconjunto de las funciones normales y convexas de M). En este trabajo se introducen dos operaciones binarias sobre M, diferentes a las dadas por Walker et al., y se estudian varias propiedades de las mismas, con el objeto de de...

  14. Consideraciones criminológicas sobre las bandas juveniles

    OpenAIRE

    Medina, Juanjo

    2010-01-01

    Este artículo ofrece una serie de reflexiones originales sobre la definición de las bandas juveniles y resume algunos de los debates claves en el campo de investigación sobre bandas. En particular, se presta especial atención a los estudios sobre la relación entre bandas y delincuencia, las teorías que explican la formación de las bandas y los factores que empujan a los jóvenes a vincularse a las mismas, los estudios sobre respuestas de política criminal a este fenómeno, ...

  15. Nivel de conocimientos del personal de salud sobre cuidados paliativos

    OpenAIRE

    2012-01-01

    Antecedentes: la medicina paliativa no figura en las prioridades de salud de México; en las instituciones existe un gran desconocimiento sobre las necesidades físicas, psicológicas, sociales y espirituales del paciente en estado terminal y sobre las de su familia; se reconoce que los profesionales de la salud carecen de conocimientos sobre cuidados paliativos. Objetivo: evaluar el nivel de conocimientos que sobre cuidados paliativos tiene el personal de salud de los tres niveles de atención m...

  16. Módulo educativo sobre emergencias escolares

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    Ana María Fajardo-Maldonado

    2009-01-01

    Full Text Available Con el fin de conocer el comportamiento ante emergencias escolares de niñas y adolescentes, se realizó una investigación descriptiva sobre el impacto de un proceso educativo de un programa de salud al colegio. El estudio se adelantó entre el 2007 y el 2008, en la localidad de Suba, en Bogotá, con un grupo de 120 estudiantes, de 6 a 18 años, de estratos cero, uno y dos. Mediante una metodología lúdica, pedagógica y de experiencias se evaluaron los conocimientos impartidos a esta población, en la que se observaron cambios en los estilos de vida y hábitos individuales y grupales. El 95% de las niñas validaron los conocimientos en salud sobre el manejo de emergencias escolares, el 100% de la población participó en la construcción del plan y trabajó en las prácticas, lo que crea conciencia acerca de la importancia del programa de salud escolar con el desarrollo de este tipo de planes.

  17. Debate sobre estudios postcoloniales y subalternos

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    David Morris

    2001-01-01

    Full Text Available A partir de los comentarios a la reseña elaborada por Ian S. Mclean sobre el libro: Cuerpos incas y el cuerpo de Cristo. El Corpus Christi en Cuzco colonial, Perú, escrito por Carolyn Dean, surge un debate sobre los Estudios Postcoloniales y Subalternos, que se desarrolló entre el 12 de agosto y el 3 de septiembre de 2001, a través de la lista de correo electrónico de H-Latam, grupo de discusión dedicado a la historia latinoamericana. En él participaron destacados especialistas en el tema, quienes analizaron, entre otras cuestiones, el uso de la jerga y las formas de escritura, la novedad de sus planteamientos, sus influencias filosóficas y epistemológicas, su pretensión de captar el punto de vista de los vencidos y subordinados, la pertinencia de unas ideas originalmente desarrolladas para el estudio del colonialismo inglés y luego aplicadas al colonialismo español en América Latina.

  18. sobre la representatividad democrática

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    Adriana Gallo

    2008-01-01

    Full Text Available En este trabajo se evalúan los efectos que reviste la aplicación de tres instrumentos sugeridos o implementados, a partir de las últimas reformas político-institucionales –primarias abiertas, sistema de doble vuelta, y reelección inmediata– sobre la representatividad partidaria en Argentina, Chile y Uruguay. La relevancia del análisis de estos mecanismos en conjunto se debe a que, por un lado, cada uno concierne a una de las tres instancias del proceso electivo trifásico –selección, elección y reelección, respectivamente–; y, por otro, los tres comparten objetivos similares que sintéticamente son: dotar de más herramientas de discernimiento político a los ciudadanos, proveer de mejores condiciones democráticas a los partidos e incrementar la legitimidad electoral del presidente. Luego del análisis comparativo de tres países con trazos diferenciados, se concluye que los costos ocultos que pueden suscitarse con cada mecanismo analizado superan a los beneficios potenciales de alguno de ellos, operando negativamente sobre la representatividad democrática.

  19. Hábitos de consumo sobre la cerveza

    OpenAIRE

    Velasco Matilla, Débora

    2015-01-01

    Estudio de los hábitos de consumo de cerveza en un pueblo determinado situado en la provincia de Segovia, Cantalejo. Contiene un resumen sobre la historia de la cerveza y su tipología y un estudio de mercado sobre la cerveza partiendo de una encuesta. Grado en Comercio

  20. Violência sobre Crianças

    OpenAIRE

    Sacadura, Catarina Brito; Aragão, Maria José

    2014-01-01

    Trata-se de um estudo de revisão sobre a violência exercida sobre crianças, os agentes da violência, os sujeitos da violência, os factores de risco, as consequências na idade adulta e a recidividade de violência sexual.

  1. Generalizaciones y creencias sobre el autismo: estudio descriptivo

    OpenAIRE

    Rodríguez Fernández, Patricia

    2015-01-01

    La autora propone una investigación sobre las creencias más generalizadas en diferentes aspectos del autismo: etiología, características, escolarización y pronóstico en diferentes grupos de edades con distinta familiaridad sobre este trastorno y con distinto nivel educativo general.

  2. Neruda e Drummond: um olhar sobre Stalingrado

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    Moacir Lopes de Camargos

    2015-07-01

    Full Text Available Este artigo tem por objetivo promover uma aproximação entre dois expoentes da lírica latino-americana do século XX, a saber: Pablo Neruda e Carlos Drummond de Andrade, a partir da análise dos poemas Canto a Stalingrado (1942 e Carta a Stalingrado (1943. Apresentaremos a visão histórica desses poetas sobre a Batalha de Stalingrado, considerada um dos principais eventos da Segunda Guerra Mundial. Ademais, será abordada a questão do engajamento político, que envolve a produção poética dos referidos autores.

  3. Zubiri et alii sobre la consciencia

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    Solari, Enzo

    2016-08-01

    Full Text Available In recent literature on science and philosophy related to consciousness, the description and analysis are not always appropriate for the phenomenical consciousness that one wants to explain. From this one can sustain, contrary to some approaches to neuroscience and analytic philosophy, that the fact of consciousness previously requires a better phenomenological description and a more precise conceptual analysis.En la reciente literatura científica y filosófica sobre la consciencia no siempre se describe ni analiza adecuadamente cuál es esa consciencia fenoménica que se desea explicar. De ahí que se argumente, contra ciertas aproximaciones de la neurociencia y la filosofía analítica, que el hecho de la consciencia necesita previamente de una mejor descripción fenomenológica y de una más ajustada analítica conceptual.

  4. Algunas reflexiones sobre el turismo cultural

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    Claudia Toselli

    2006-01-01

    Full Text Available El presente artículo, si bien no pretende hacer un análisis exhaustivo sobre el turismo cultural, tiene por objetivo presentar algunas reflexiones acerca de esta modalidad turística desde diferentes aspectos. En primer lugar se abordará la relación entre turismo cultural y globalización, para luego centrarse en la incidencia de esta actividad como factor de desarrollo local y regional, haciendo referencia a algunos ejemplos de Argentina. Intentará también introducir acerca de los aspectos positivos y negativos, o puntos fuertes y débiles del turismo cultural. Y por último, esbozará algunas consideraciones acerca del rol de las organizaciones internacionales y de las principales agencias de cooperación internacional, incluyendo las resoluciones, declaraciones y recomendaciones más significativas en este campo del turismo.

  5. NOTAS SOBRE A LÓGICA ESTOICA

    OpenAIRE

    Valter Duarte Moreira Júnior

    2014-01-01

    Diante do grande desenvolvimento da Lógica e do cálculo proposicional no século XX, estudiosos do assunto em todo o mundo se concentraram em um rigoroso estudo dessa disciplina e sua história. Nesse processo, o eminente lógico polonês Jan Lukaziewicz descobriu em sua pesquisa sobre história da lógica a Lógica Estoica, que, já em III a. C., havia antecipado diversos métodos e conceitos da Lógica Moderna. Nossa intenção neste artigo é fazer um breve esboço da lógica desenvolvida pelos Estoicos....

  6. Sobre el Boletín

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    Andrés Arias

    2007-04-01

    Full Text Available En la página de Internet de la Biblioteca Luis Ángel Arango se indica que una de la funciones principales del Boletín es la de reseñar la novedades bibliográfica que van apareciendo en el país. "Novedades": de lo nuevo, tanto en las publicaciones como en las reseñas. Es decir, si lo que asegura la página de la Biblioteca es cierto, quien se acerca al Boletín debe encontrarse con comentarios recientes sobre libros recientes. Con guía que le ayuden a elegir entre las mil ofertas que hay en el mercado bibliográfico. Pero bien sabemos que este objetivo no se cumple.

  7. Conceptos fundamentales sobre el mantenimiento de edificios

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    Juan Miguel Arencibia Fernández

    2007-01-01

    Full Text Available En esta investigación se realiza una profunda búsqueda sobre el tema del Mantenimiento de Edificios donde se abundan diferentes conceptos y criterios de la misma, encontrándose de esta forma los más utilizados métodos a emplear. Luego teniendo en cuenta las clasificación del Mantenimiento de Edificio según los tipos de obras, propietarios del inmueble y el momento que éste se realiza, como los costos de una edificación y su equipamiento a utilizar. Otros de los aspectos a tener en cuenta es la organización del Mantenimiento su planificación y responsabilidad, las ventajas que puede traer tanto para la economía y para el mismo usuario, como su vida útil, para el cual fueron ejecutadas las edificaciones.

  8. Refletindo sobre as redes sociais digitais

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    Sônia Cristina Vermelho

    2014-03-01

    Full Text Available Este artigo apresenta os resultados da primeira etapa da investigação realizada pelo grupo de pesquisa Ciência, Tecnologia e Sociabilidade do Mestrado em Promoção da Saúde. Nele situamos a área da Promoção da Saúde; em seguida apresentamos uma discussão em torno do conceito de "redes sociais digitais" e, finalmente, apresentamos alguns resultados da pesquisa bibliográfica realizada nesta etapa. Os dados bibliográficos encontrados (artigos, teses e dissertações indicaram um aumento de trabalhos publicados sobre o tema a partir de 2005, bem como uma concentração nas áreas de Comunicação e Educação.

  9. NOTAS SOBRE A LÓGICA ESTOICA

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    Valter Duarte Moreira Júnior

    2014-02-01

    Full Text Available Diante do grande desenvolvimento da Lógica e do cálculo proposicional no século XX, estudiosos do assunto em todo o mundo se concentraram em um rigoroso estudo dessa disciplina e sua história. Nesse processo, o eminente lógico polonês Jan Lukaziewicz descobriu em sua pesquisa sobre história da lógica a Lógica Estoica, que, já em III a. C., havia antecipado diversos métodos e conceitos da Lógica Moderna. Nossa intenção neste artigo é fazer um breve esboço da lógica desenvolvida pelos Estoicos.

  10. Reflexiones sobre el tratamiento ambulatorio de drogodependientes.

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    Juan Carlos González García

    1989-01-01

    Full Text Available Intentamos plantear algunas de las innumerables contradicciones, paradojas y mitos que se presentan en relación con las demandas y con las necesidades terapéuticas de los consumidores de drogas, desde el nivel de atención extrahospitalaria pública y dentro de una Unidad de Salud Mental. Se intenta reflexionar sobre los conocimientos teóricos y la realidad práctica, su interdependencia, complementariedad y divergencia, con la finalidad de evitar la huida asistencial o la omnipotencia terapéutica. Se valoran las diferencias existentes entre la farmacología teórica, anclada en el paradigma lewiniano, y la realidad clínica.

  11. Documentos sobre aniversarios, reparticiones y capellanes

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    José Luis Martín Rodríguez

    1989-01-01

    Full Text Available En el Cómpoto o Arte de contar del cabildo segoviano  se habla de la mayordomía de Reparticiones que administra los bienes dejados para la celebración de aniversarios por el alma de los benefactores de la iglesia, y es de suponer que el volumen alcanzado por este tipo de donaciones llevaría a los demás cabildos peninsulares a individualizar estas rentas y a incluir en los libros de Obituarios o Aniversarios las obligaciones contraías por el cabildo, es decir por las dignidades o personas, canónigos y racioneros, que forman el cabildo en sentido estricto, y por los auxiliares de éstos: capellanes, sochantre, mozos de coro, organista, portero, campanero, refitolero, barrendero, perrero... sobre cuyas obligaciones y situación estamos mal informados.

  12. Sobre a moralidade do direito em Kant

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    Joãosinho Beckenkamp

    2009-07-01

    Full Text Available http://dx.doi.org/10.5007/1677-2954.2009v8n1p63Na atual literatura sobre a fi losofi a prática de Kant há uma tendência a negligenciar a distinção feita na Metaphysik der Sitten entre direito (Jus e ética (Ethica, a ponto de ser a doutrina do direito de Kant vista como uma mera aplicação do imperativo categórico. Este artigo procura mostrar que Kant apresenta em sua doutrina do direito uma série de argumentos que logram estabelecer o direito de modo inteiramente independente da ética.

  13. Apuntes sobre el Cultivo del Cacao

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    Ochoa Horacio

    1940-03-01

    Full Text Available Por muchos años permaneció el cultivo del cacao en nuestro país, en un deplorable atraso técnico; el agricultor a duras penas si tenía conocimientos generales de su explotación y, de una industria que tuvo su auge debido a la extensividad de las plantaciones, hoy sólo resta una reducida porción de ella. ¿Qué factores operaron esa tan notoria reducción hasta el punto de que exportadores en otros tiempos nos convertimos en importadores obligados de ese producto? Hay muchas razones de orden técnico y de orden económico. Los cacaotaleros obtenían halagadoras retribuciones cuando el jornal consistía en la alimentación que se le daba a la peonada y cuando, generoso, añadía una sobretasa en dinero. Bajo ese sistema de explotación, el descuadre que pudiera ocasionar tanto las enfermedades criptogámicas como los plagas, no era suficiente como para provocar el pesimismo en el hacendado y por tanto inducirlo al abandono de la plantación. A medida que las condiciones sociales del campesino se fueron modificando y su standard de vida aumentaba, cambió fundamentalmente el mecanismo de la industria Se observó entonces que los árboles morían y que era pobre la producción; que lo que en tiempos pasados fuera un negocio lucrativo, se les había convertido en una actividad improductiva y, para controlar estos fenómenos que se generalizaban en todas las zonas cacaotaleras, opusieron la ignorancia, el rutinarismo y la desidia. Lentamente se han ido modificando los conceptos antiguos sobre el trabajo cultural y los cuidados que con relación a las enfermedades y plagas requiere este delicado cultivo; la rutina está echando paso atrás y las experiencias científicas que procuran la prosperidad del plantío, se ponen en práctica en algunas zonas . Esto da esperanza para la reconstitución de la industria cacaotalera en nuestro país, que cuenta con inmensas regiones de óptima calidad para hacerla florecer sobre bases nuevas, con

  14. Aspectos da pesquisa sobre tipologia textual

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    Luiz Carlos Travaglia

    2014-03-01

    Full Text Available Este artigo chama a atenção dos pesquisadores sobre tipologiatextual, na área da Linguística Textual, sobre aspectos que sãoimportantes para que estas pesquisas gerem conclusões egeneralizações mais confiáveis. Os pontos comentados são: a anecessidade de uma teoria tipológica geral de textos que inclusivedetermine se as categorias de texto são todas da mesma natureza. Oautor propõe uma teoria geral; b a necessidade de estudos maishorizontalizados ou amplos que estabeleçam campos de pesquisa ede estudos mais verticalizados, que aprofundam o conhecimentode pontos específicos; c os aspectos metodológicos para superarproblemas no levantamento das categorias de texto existentes emuma sociedade e cultura, em suas diversas comunidades discursivase esferas de ação social; d a questão dos parâmetros e critérios paraconsiderar uma categoria de texto como sendo uma categoria e seela é de determinada natureza; e a questão dos critérios e parâmetrospara a caracterização das categorias de texto; f a necessidade deestudar as diversas relações de diferentes tipos que podem ocorrerentre as categorias de texto (de diferentes naturezas, tanto em suacomposição quanto em seu funcionamento sociodiscursivo; e g aatenção que merece a questão teminológica.

  15. Reflexiones sobre la crisis. Vidas y Memorias

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    Estela Vázquez

    2009-11-01

    Full Text Available El artículo se plantea analizar la deslegitimación de la política y de los políticos, así como del acto de votar. Encuadrado en el marco del contexto de la crisis argentina de los últimos años, quiere responder, por medio de la entrevista oral y la historia de vida, a cómo se ve desde la ciudadanía el ejercicio de la democracia, las prácticas políticas, la alternancia en el poder y de qué forma todo esto influye en las vidas personales de los entrevistados. Asimismo, se entrelaza el análisis del espectáculo político con el público que ofrecen los medios de comunicación y de entretenimiento, con lo que se viene a concluir que es cada vez más difícil distinguir en el debate social la ficción de los hechos y personajes verdaderamente reales. Para este trabajo se analizan tres trayectorias laborales de ex-empleados de la administración pública, y sus apreciaciones sobre el despido, vinculando estas experiencias de vida a la formación de sus ideas sobre la política y los políticos.. Sus lugares de trabajo eran el Registro Civil, la Secretaría de Educación y la Legislatura, respectivamente.__________________ABSTRACT:This article tries to analyse the discredit of politics and politicians along with the act of voting. Placed in the context of the Argentine crisis, it wants to determinate through oral interview and life story how citizens see the exercise of democracy, the political practices and the power switching, to establish how they have influenced in the lifes of the persons being interviewed. At the same time, it interlaces an analysis of he political spectacle shown to the public by the media, to conclude that is becoming more a more difficult to distinguish the fiction from the real characters in the social debate.

  16. El puente Bendorf sobre el Rin (Alemania

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    Hirschfeld, Kurt

    1966-03-01

    Full Text Available This recently completed bridge over the Rhine is 524 m long, and is a reinforced concrete structure, built by successive overhangs, without the aid of formwork or scaffolding. The centre span is 208 m in length, and the other six vary between 43 and 71 m. The deck consists of two independent parts, including a continuous box girder, with a hinge at the crown of the main span. It is stiffened with transversal diaphragms, spaced every 34 m. The piles rest on pneumatic caissons, which go down to 16 m below the river bed. The attachment between the piles and the caissons is rigid, at the central pile, and at the other piles it operates through a roller bearing. The central span was constructed without intermediate supports for the first 40 m, and beyond that stage, for greater safety, a provisional movable tower was built, as a temporary point of support for the free overhang. The various sections were concreted and post-tensioned with 32 mm bars, by the Dywidag procedure. Account was taken of the varying deformations that took place at each section, so as to add the necessary counter-deflection that finally enabled the two sides of the arch to meet at the crown at the calculated point.Este puente, recientemente construido, sobre el Rin, tiene una longitud total de 524 m, es de hormigón armado y se ha realizado por el procedimiento de voladizos sucesivos, sin entramado auxiliar ni cimbras. La luz del tramo central es de 208 m, y las de los otros seis oscilan entre 43 y 71 m. La estructura del tablero se compone de dos partes independientes, formadas por una viga tipo cajón, continua, con una articulación en la clave del tramo central y rigidizada con diafragmas transversales espaciados a 34 m. Las pilas centrales se apoyan sobre cajones neumáticos que descienden 16 m debajo del fondo del río. La unión entre estas pilas y la estructura cajón es rígida, y de tipo rodillo en las otras pilas. El tramo central se ha construido por voladizos

  17. Algunas reflexiones sobre el poder itinerante almohade.

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    Maribel FIERRO

    2009-12-01

    Full Text Available La primera parte del artículo consiste en la presentación de dos estudios sobre el poder itinerante en el Magreb: Jocelyne Dakhlia (1988 para las épocas hafsí y posteriores; Manuela Marín (2005 sobre el caso almohade. En la segunda parte, primeramente se analiza la convergencia del itinerario de Ibn Tumart con las conquistas de ‘Abd al-Mu’min: el movimiento –real o legendario– del fundador mesiánico del imperio marca el territorio conquistado por su sucesor el califa y su presencia inserta el espacio en la memoria, la geografía y el poder imperiales. A continuación, se plantea la compleja cuestión de la «capitalidad» almohade: se puede hablar de una capital sagrada (Tinmal, una capital central (Marrakech, capitales regionales (Sevilla, Túnez y de una nueva capital inacabada (Rabat. Pero es la presencia (hadar del propio califa la que determina dónde está el centro del imperio.La première partie de cet article présente deux études sur le pouvoir itinérant au Mahgreb : celle de Jocelyne Dakhlia (1986 sur la période hafside et les suivantes, celle de Manuela Marín (2005 sur le cas almohade.Dans la seconde partie, on analyse d’abord la convergence de l’itinéraire d’Ibn Tumart avec les conquêtes d’‘Abd al-Mu’min : le  trajet – réel ou légendaire – du fondateur messianique de l’empire marque le territoire conquis par son successeur le calife et sa présence ancre l’espace dans la mémoire, la géographie et le pouvoir impériaux. Ensuite, on examine la question complexe de la « capitalité » almohade : on peut parler d’une capitale sacrée (Tinmal, d’une capitale centrale (Marrakech, de capitales régionales (Séville, Tunis et d’une nouvelle capitale inachevée (Rabat. Mais c’est la présence (hadar du calife lui-même qui détermine où est le centre de l’empire.

  18. Cassirer e Sartre sobre o esclarecimento

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    Vinicius de Figueiredo

    2005-12-01

    Full Text Available O presente ensaio levanta alguns aspectos da estética no século XVIII. Partindo da apresentação de duas interpretações hoje clássicas sobre o assunto (a de Cassirer e a de Sartre, busca rever o pressuposto analítico comum a ambas, para, em seguida, apontar as conseqüências que a relação entre autor e público trazem para a compreensão de aspectos estilísticos que nos parecem centrais para a literatura do Esclarecimento.This paper aims at throwing some light on the Eighteenth-Century aesthetics. After examining two classical interpretations - Cassirer e Sartre's - concerning this subject, I argue that both authors share a common analytical pressupposition. My main purpose is to show that without taking account of the the relationship between author and public, we cannot understand some essential characteristics of the literature in the Enlightenment.

  19. Considerações sobre Agamben

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    Luiz Hebeche

    2012-12-01

    Full Text Available http://dx.doi.org/10.5007/1677-2954.2012v11n3p329Giorgio Agamben em pequenos artigos, entrevistas e baixo-assinados tem chamado a atenção para o estado de abandono do povo palestino, mas curiosamente esse assunto não é tratado em suas obras mais importantes. Em Homo sacer, por exemplo, ele retoma o termo “muçulmano”, comumente empregado pelos prisioneiros dos campos de concentração como a expressão do maior abandono possível da existência humana, mas não reflete suficientemente sobre a origem dessa palavra, isto é, de que nessa expressão já se encontrava o ódio e o desprezo do sionismo, não só entre os próprios prisioneiros, mas pelo povo palestino e pelos árabes em geral. A seguir comentaremos brevemente algumas obras desse autor que, dando continuidade à ideologia da vitimação, faz com que, mais uma vez, o passado inominável acabe encobrindo o presente abominável.

  20. NOTAS SOBRE A IRRACIONALIDADE DA GUERRA

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    Edilene Leal

    2014-08-01

    Full Text Available Nesse artigo, procedemos a uma análise sobre a irracionalidade da guerra em tempos cada vez mais caracterizados pela evolução da racionalidade técnica, ou seja, em tempos contemporâneos. Esse é o ponto a partir do qual traçamos, aqui, um percurso argumentativo dividido em duas partes. Na primeira, reconstituímos elementos da concepção de guerra de filósofos como Maquiavel, Clausewitz e Kant, destacando, nos dois primeiros pensadores, a prerrogativa de que a guerra é racional quando se configura meio para fins políticos. Na segunda parte, confrontamos essa racionalidade política da guerra com a dupla dimensão técnica que a guerra assumiu: de um lado, a guerra como um fim em si mesmo e não mais como meio da ação política; de outro, a dimensão racionalizante da guerra como resultado do progresso técnico e científico, atualizada como guerra do terror.

  1. Sobre a "conveniência" da escola

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    Marisa Vorraber Costa

    2009-01-01

    Full Text Available El artículo pretende mostrar un nuevo "uso" para la escuela, vinculado a la lógica de la etapa actual de la globalización en la que la cultura ocupa una posición singularmente importante, entrelazándose en la vida social, en los circuitos económicos y en los regímenes políticos. Recurriendo a la teorización de George Yúdice sobre los usos de la cultura en la era global, se analizan tres proyectos sociales - Você Apita, Tim Música nas Escolas y Escola Aberta - considerados expresivos de la forma como empresas, organizaciones públicas e iniciativas de la sociedad civil se articulan con la cultura y con la educación para hacer con que los mercados globalizados se muevan, pero no sólo en sus propios intereses, aunque ellos persistan en la cima de las prioridades. Hay indicios de que la "conveniencia" de la escuela sobrepasa el interés mercantil inmediato, y se proyecta como estrategia dirigida hacia una variada gama de propósitos socio-políticos, económicos y culturales. Entre ellos, la posibilidad de gobernar la vida de las personas, forjando visiones de mundo y de sociedad.

  2. Consideraciones sobre de la ética

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    Enrique Cantolla Bernal

    2005-12-01

    Full Text Available El presente ensayo muestra como el hombre, a través de la historia, a estado constantemente buscando beneficios para su vida y ha debido cumplir con varias tareas, que con el tiempo han dado origen a costumbres y comportamientos que han llevado a constituir la moral y la ética. Esta última, nos permite definir y determinar los principales fines de la vida del hombre y cuales son los principios y normas que lo rigen. Así, el estudio de la ética nos plantea la interrogante sobre "los fines de la vida en este mundo" y cuales son las conductas para conseguirlos. Sin embargo, el autor plantea que el hombre se enfrenta a la elección de valores alternativos y ante la interrogante de ¿cuál es el sumo bien del ser humano y de la sociedad?, han surgido diversas teorías que muestran como, casi todas las sociedades, han elegido la búsqueda de la felicidad como "el sumo bien", pero este concepto de "la felicidad" ha variando producto de las diversas teorías y doctrinas, que han emergido a lo largo de su historia.

  3. Puente sobre el Rin Dusseldorf, Alemania Federal

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    Modemann, H. J.

    1980-05-01

    Full Text Available The bridge over the Rhine, in Dusseldorf- Flehe — one of whose most important jobs is to alleviate traffic from the other bridges in the town of Dusseldorf — consists of three clearly diferentiated parts: — a metal part 368 m. in length; — another in prestressed concrete, 780 m. long, and — an A-shape pylon, acting as element joining both. This bridge has the span clearance record in West Germany, and its metal part constitutes the longest corbel in the world.

    El puente sobre el Rin, en Düsseldorf- Flehe —una de cuyas funciones más Importantes es la de aliviar el tráfico a los demás puentes existentes en la ciudad de Dusseldorf—, consta de tres partes claramente diferenciadas: — una metálica de 368 m de longitud; — otra de hormigón pretensado de 780 m de largo, y — un pilono, en forma de A, que sirve como elemento de unión entre ambas. Este puente ostenta el récord de luz libre en la República Federal Alemana, y su parte metálica constituye el voladizo de mayor longitud del mundo.

  4. sobre la población

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    Mauricio Schoijet

    2005-01-01

    Full Text Available En este artículo se analizan varios elementos de la obra de Thomas Malthus sobre población, en particular la recepción de su teoría y su posición política. Se resumen las críticas políticas, ideológicas y científicas que ha recibido, y se examinan asuntos hasta ahora no tratados por otros comentaristas, como es el caso de las excepciones admitidas por Malthus respecto al papel de las relaciones sociales feudales en la agricultura de Polonia y Rusia. Se sugiere que pese a que su teoría tendía a reforzar la pesada represión social y sexual existente, Malthus no fue apoyado por la burguesía británica (contrariamente a la apreciación de Marx. Se deduce que Malthus no percibió cabalmente los avances de la agricultura, y se refuta la tesis de Donald Winch de que habría sido un liberal de izquierda en política pues si bien en algunos aspectos se le puede considerar progresista, en otros, esenciales, conservador extremadamente represivo o protofascista, incluso precursor de la teoría de los golpes de Estado.

  5. SOBRE UNA NUEVA PROPUESTA DEL PROCESO EVOLUTIVO

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    LUIS ALVARO CADENA MONROY

    2009-01-01

    Full Text Available Se mencionan algunas debilidades de tres propuestas alrededor del proceso de evolución de los organismos: gradualismo, neutralismo y equilibrio puntuado. Aunque dos de ellas hacen referen- cia a tasas de cambio, y la otra (neutralismo se refiere a la ausencia de la selección a nivel mi- croscópico, se toman estas tres propuestas debido a la relevancia que tienen en el contexto de la bibliografía sobre la evolución. Se hace una propuesta en la que se busca integrar estos puntos de vista, resolviendo las debilidades de cada propuesta. Para ello, se sugiere que el proceso evolutivo fundamentalmente tiene dos etapas: en la primera etapa, los organismos crean sus nichos ecoló- gicos. En este período, la iniciativa selectiva es de los organismos (“selección invertida”. Posterior- mente, el proceso puede invertirse, y será el nuevo nicho el que seleccione las variantes de los organismos que se le adaptan (“selección normal”. Este último proceso será posible, solamente si la “selección invertida” puede inscribirse en una tendencia de cambio evolutivo más general. Se discuten dos ejemplos en que se aplica la noción de “selección invertida”: el origen de los cordados y la evolución hacia el Homo sapiens.

  6. Apuntes sobre el Cultivo del Cacao

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    Chavarriaga. Eduardo

    1940-09-01

    Full Text Available El sombrío constituye uno de los problemas que se presentan en el cultivo del cacao, de necesaria resolución para ayuda del agricultor en su labor al producir tan importante grano. Contribuye a la economía del cultivo un conocimiento amplio de la cuestión que debe preocupar al productor inquiriendo no solamente por lo acostumbrado en la región, sino por lo que en otras partes se conoce sobre el particular, para abandonar un poco el emperismo y hacer una industria cada día más próspera. Como razón a favor del sombrío, tenemos los países en donde se produce cacao de fina calidad; Trinidad, Venezuela. Nicaragua, siempre acostumbran la sombra; y sin ir muy lejos, en el Valle del Cauca, donde no se cultiva a pleno sol y se obtienen plantaciones de larga duración y producto superior, es bien conocido el beneficio de ella; allí, los agricultores conocen los desastrosos efectos que ocasiona la falta de sombra, cuando por cualquier causa mueren los árboles que la suministran. Por tanto, en este estudio indicaremos el uso de la sombra para el cacao, como el medio de conservar la fertilidad y buenas condiciones del suelo, obtener buenos rendimientos y asegurar larga vida a la plantación.

  7. Sobre a diversidade de sentidos de comunidade

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    Silva, Rosalina Carvalho da

    2005-01-01

    Full Text Available Nosso objetivo neste trabalho foi realizar algumas reflexões sobre as implicações da expansão do uso do termo comunidade e sua diversidade de sentidos. Pretendemos discutir os usos indiscriminados do termo nem sempre acompanhados da devida reflexão e crítica. A partir das perspectivas da sociologia, saúde e psicologia social identificamos que o termo ainda permanece arraigado aos ideais de comunidades naturais próprios aos períodos do feudalismo e do pós-revolução industrial. Esta concepção pode camuflar formas impositivas de trabalho em muitos dos quais o termo comunidade é empregado praticamente como sinônimo de população-alvo e participação comunitária é vista meramente como freqüência às atividades propostas por profissionais que determinam quem são as comunidades. Comprometem-se assim, as práticas emancipatórias. Para repensarmos comunidades, hoje, consideramos necessário relevar os aspectos processuais relacionais, dialógicos e identitários em constantes mudanças. Assim, a condição de comunidade extrapola a delimitação geográfica para as possibilidades de pertencimento e participação através dos espaços simbolicamente delimitados

  8. Representaciones sociales sobre riesgo y vulnerabiidad

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    Pedro Humberto Faria CAMPOS

    2012-08-01

    Full Text Available O artigo traz uma reflexão teórica sobre a importância da Teoria das Representações Sociais para explicar fenômenos sociais na área da saúde e seu papel metodológico na compreensão de comportamentos e práticas relacionadas ao campo da saúde assim como o potencial transformador das representações para prevenção e o cuidado com a saúde das coletividades humanas. Revisitando os conceitos de práticas sociais e analisando o papel normativo que designa as representações sociais, se discute a mudança social e os fatores sócio-cognitivos a ela associados. Do ponto de vista heurístico destaca a necessidade de identificar a estrutura de uma representação, condição essencial para a comparabilidade.

  9. Sobre manhãs, humor e atos de fala

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    Joana Plaza PINTO

    Full Text Available RESUMO Este ensaio apresenta uma trajetória de leituras de Frege a Austin, intermediadas pela interpretação independente e ousada de Kanavillil Rajagopalan. Ao argumentar sobre uma gama de aproximações teóricas entre Frege e Austin, este ensaio narra a importância da interpretação de Rajagopalan para o debate sobre Austin no Brasil.

  10. Recursos sobre emergencias y ayuda humanitaria en internet.

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    López Miguel, Daniel

    2007-01-01

    Dada la enorme cantidad de organismos, agentes y personas que llegan a participar en las emergencias, la información y documentación es muy dispersa y diversa, desde organismos oficiales y gubernamentales hasta servicios de noticias, pasando, por supuesto, por organizaciones no gubernamentales. Con el presente artículo pretendemos aportar unas pinceladas sobre los recursos que pueden encontrarse en Internet sobre los conflictos y crisis humanitarias. Peer Reviewed

  11. Liberalismo versus republicanismo: notas sobre o conceito de liberdade.

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    Felipe Nunes

    2007-01-01

    Full Text Available Este artigo tem como objetivo contrastar as perspectivas liberal e republicana sobre o conceito de liberdade. Através da comparação feita entre os dois modelos tecemos considerações sobre a realidade brasileira tendo como foco o atual estado de crises advindas dos casos de corrupção no país.

  12. Bibliografía comentada sobre la historia de Guanacaste

    OpenAIRE

    Solano-Muñoz, Edgar

    2010-01-01

    El texto presenta una bibliografía comentada relacionada con Guanacaste. El propósito del texto es hacer una valoración desde la óptica de la historia regional sobre los textos escritos sobre historia de Guanacaste considerando cuatro aspectos: 1. representaciones sociales, 2. estudios coloniales, 3. historia regional y 4. historia local. Universidad de Costa Rica- Sede Guanacaste

  13. Reflexiones sobre Enfermería

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    Inés Durana Samper

    1992-12-01

    Full Text Available

    agosto 6 de 1992

    Presentación ante la Academia Nacional de Medicina
    Señores miembros de la Junta Directiva, académicos, enfermeras, señores, señoras:

    Introducción
    Para ingresar como Miembro Asociado de esta ilustre Academia Nacional de Medicina, quiero exponer algunas reflexiones sobre la evolución en Colombia de la enfermería, mi profesión, ejercida ininterrumpidamente en estos últimos cuarenta años. Amanera de colofón, me referiré a la función esencial de la enfermera, es decir a su consagración al arte de cuidar.

    La metodología aplicada en este trabajo incluye la revisión de archivos, la lectura de publicaciones y, además, algunas entrevistas con personas que pacientemente escucharon mis preguntas y ampliaron mi información con valiosos comentarios.

    Mis agradecimientos van, en primer término, al académico Fernando Serpa Flórez, quien ha tenido la iniciativa de presentarme a la Academia Nacional de Medicina; también va para aquellos académicos que respaldaron mi aceptación en esta prestigiosa sociedad científica. Igualmente expreso mi reconocimiento al académico Héctor Pedraza Mendoza, autor del primer libro conocido sobre reseña histórica de la enfermería en el país, y a quien correspondió, como funcionario de la administración pública, la orientación y control de la profesión en su cargo de Jefe de la División Infantil y Materna del entonces Ministerio de Trabajo, Higiene y Previsión Social. Libro citado con frecuencia’ durante el trabajo.

    Mis agradecimientos también a mis profesores, y entre ellos, en especial, al académico Alberto Albornoz- Plata. No puedo pasar por alto la memoria de los siempre recordados, doctores Jorge Boshell Manrique y José Pablo Leyva, quienes, desde la Escuela Superior de Higiene, supieron orientarme en la transformación académica del

  14. Contando historias sobre contadores de historias

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    Tomás Ibáñez Gracia

    2002-06-01

    Full Text Available Hay personas que nos han dejado como legado un texto. Su texto. A veces, una obra ingente, fecunda, imprescindible. Hay otras que nos han dejado además la palabra, su palabra, su vida. Rex Stainton-Rogers es una persona del segundo tipo. Un ser humano excesivo, brillante, emprendedor, vivo. Su palabra es su herencia. Su legado, algunos y algunas de los más relevantes científicos sociales de la emergente generación, sobre todo en Gran Bretaña, que han sido personas educadas, entrenadas, formadas por esta figura excepcional. Su trabajo, más allá de los textos que nos ha legado, ha consistido especialmente en mostrar la vida, el laboratorio donde se forja realmente el conocimiento. Y así, quienes pudieron compartir una parte de su vida con él, han tenido el privilegio de estar en contacto con todo aquello que hace apasionante el saber, aquello que lo hace útil, aquello que te emborracha de placer. Rex murió en febrero de 1999 del mismo modo en que vivió, libre, espléndido. Un año más tarde, su compañera Wendy Stainton-Rogers organizó junto con algunas de las personas que más deben a la vida de Rex, un simposium para conmemorarle a él, a su vida y a su palabra. Ésta es la contribución que nosotros hicimos a ese homenaje.

  15. Puente sobre el Rhin – Suiza

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    Bänziger, D. J.

    1972-10-01

    Full Text Available In the brief period of two months a new bridge over the Rhine has been constructed to substitute one which collapsed on August 1970, because of the undermining of one of its pillars. On November 28 1970 the new bridge was opened to the traffic, including trucks of up to 3.5 Kp, and on December 7th of the same year vehicles of up to 26 Kp could use it. In the construction of this bridge, for the first time, use has been made of cylindrical, prefabricated, precast thin shells. This project is of special significance because of its operational importance, since it connects Lichtenstein and Switzerland; the exceptionally short time of its construction; and the new type of deck used.Para sustituir un puente sobre el Rhin, hundido el 14 de agosto de 1970 por socavación de uno de sus pilares, se ha construido un nuevo puente en el escaso plazo de dos meses. El 28 de noviembre de 1970, este nuevo puente se abrió al tráfico de vehículos de hasta 3,5 Mp, y el 7 de diciembre del mismo año, a los vehículos de hasta 26 Mp. En la construcción de este puente se han utilizado, por vez primera, láminas cilíndricas de cubierta, prefabricadas y pretensadas. La importancia del puente, que sirve para el enlace entre el Principado de Liechtenstein y Suiza, el nuevo tipo de tablero utilizado, así como el plazo de construcción, extraordinariamente breve, dan un interés especial a esta obra.

  16. Considerações sobre o Mercosul

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    Ricardo Seitenfus

    1992-12-01

    Full Text Available Após traçar as origens históricas e conjunturais do processo de integração na Bacia do Prata, o autor analisa as características do Tratado de Assunção, através de um organograma detalhado. Na segunda parte, três questões sem resposta são colocadas aos leitores, ou seja, o processo de institucionalização envolvendo a constituição de um espaço supranacional que estará diretamente vinculado aos objetivos do processo; as relações do Mercosul com o Nafta e a definição de um quadro de cooperação e, finalmente, coloca a questão sobre o nível de participação do Estado no processo integracionista sob a ótica da necessidade de reconversão de certos setores econômicosIn the first part of this article, the author, after delineating the historical and circumstancial sources regarding the process of integration in the Prata Basin, analyses the characteristics of the Traty of Asunción (Paraguay through a detailed organizational chart. In the second part, three open questions are discussed: (a the process of institutionalization which involves the constitution of some supranationalization which invoves the constitution of some supranational space which will be closely related to the objectives of the process; (b the relationships between the Mercosul and the Nafta and the definition of a cooperative program; (c the level of participation of the State into the integrationist process under the dear need of a reconversion of some economic sectors.

  17. Reflexión sobre Rubem Fonseca

    Directory of Open Access Journals (Sweden)

    Marco Martos Carrera

    2011-05-01

    Full Text Available El día de hoy la Universidad Nacional Mayor de San Marcos honra a sustradiciones recibiendo a Rubem Fonseca, el representante más conspicuode la cultura del Brasil contemporáneo, y haciéndolo rinde homenaje a supaís originario y a la lengua portuguesa hermana de la española que esla lengua franca en nuestra nación. Expresa también el deseo evidente denuestra Facultad de Letras y Ciencias Humanas de abordar la Literatura deBrasil como parte sustancial de la Literatura Latinoamericana y no comoalgo ajeno, pero vecino, cuando decimos profesar un interés por la LiteraturaHispanamericana.Dos cuestiones de fondo en el estudio de la obra de un escritor tienensuma importancia: la cultura en la que se forma y otra, su propia biografía.La cultura del Brasil, sobre todo en el plano literario se formó en vínculo estrechocon la cultura de Portugal, pero pronto la tierra americana, las gentesnacidas aquí fueron dejando su impronta en eso nuevo que nacía y que estádejando honda huella en la cultura universal. En ese sentido un narradorcomo Machado de Assís, aludido irónicamente por uno de los personajesde los cuentos recientes de Rubem Fonseca, es en el siglo XIX, la mejor manifestaciónde talento literario de América del Sur. Su nombre se pronunciacon respeto al lado de otros grandes escritores de diferentes latitudes queson los fundadores del cuento contemporáneo: Poe, Chejov, Maupassant.

  18. Estudio sobre encofrados de madera modernos

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    de la Peña Aznar, Juan M.

    1980-12-01

    Full Text Available The author continues the development of the subject «Modern Timber Formwork», by summing up the comparative examination —already carried out in Part III of this Study— of the different types of glued timber existing on the market. In addition, the loads and stresses allovk/able for coniferous timber and the proposal for establishing Regulations for the Timber Section of the Research and Experimental Forestry Institute of Spain are given. In part V of the author's Study, published in this article, the important subject of the glues used for joining timber, a truly vital point in order to obtain louvered timber beams which are simply glued together, is approached.

    El autor continúa el desarrollo del tema sobre «Encofrados de madera modernos», resumiendo el estudio comparativo —ya hecho en la Parte III de este Estudio— de las diferentes vigas de madera encolada existentes en el mercado dando, además, las cargas y tensiones admisibles para maderas coníferas y la propuesta de Reglamentación de la Sección de Maderas del Instituto Forestal de Investigaciones y Experiencias de España. En la parte V del Estudio del autor, publicada en este artículo, se aborda el importante tema de las colas empleadas para las uniones de madera, algo realmente vital para la obtención de vigas de madera en celosía simplemente encoladas.

  19. Perspectivas sobre la comunicación

    Directory of Open Access Journals (Sweden)

    María Luisa Naranjo Pereira

    2005-01-01

    Full Text Available Existen diversas teorías, enfoques y modelos de comunicación; de cada uno de ellos pueden rescatarse aspectos de gran valor para el logro de una comunicación eficaz. Este documento presenta una revisión de esos aspectos, para que se pueda comprender mejor y enriquecer uno de los procesos más importantes para el ser humano: el proceso de comunicación. Este es un ensayo en el cual se da a conocer la posición de la autora sobre el tema abordado. Se rescata el acento del enfoque humanista en la relación humana y en las etapas donde se desarrolla. A partir de la comunicación pragmática, se analizan los axiomas comunicacionales en que se fundamenta y lo que se conoce como patologías de esos axiomas. Además se revisan las principales características que distinguen la asertividad en la comunicación y las distintas formas de manipulación que la entorpecen. Del enfoque de Virginia Satir, se presentan los diversos aspectos de la comunicación, tanto funcional como disfuncional y los modelos de comunicación propuestos por esta autora. Se revisan aspectos importantes del enfoque de la comunicación educativa, tales como el control de la distancia, la voz y el cuerpo, las palabras y el situacional; así como los elementos que bloquean el proceso de comunicación y las sugerencias acerca de formas correctas de escuchar. Además, se hace referencia a la necesidad de emplear procesos educativos más holísticos y constructivistas y una comunicación-interrelación horizontal y con equidad.

  20. Sobre el genio de las mujeres

    Directory of Open Access Journals (Sweden)

    Valcárcel, Amelia

    1992-11-01

    Full Text Available Not available.

    A los varones, desde Tácito a Schopenhauer, nunca les ha parecido que las verdaderas mujeres son las que tienen en casa, sino las que tienen los otros varones (los germanos, los orientales. Por su parte, Wojtyla ha hecho profesión de fe «en el genio de las mujeres», reclutando enternecidos agradecimientos como el de Macciochi. La autora del presente artículo adopta una perspectiva histórica y una actitud analítica para aclarar qué es ese genio que como genérico nos adjudican. En primer lugar, la idea de genio es romántica y va emparejada con la de destino, sobre todo en el caso de los genios colectivos. Se trata de nociones que se construyen estipulativamente con fines pragmáticos y no admiten, por definición, contraejemplos. En el caso del genio de las mujeres, los llamados «valores femeninos» que lo constituyen pertenecen al ámbito de la Siulichkeit, y su eventual convalidación en el de la Moralitiit no puede obviar el proceso consistente en la crítica y el desmarcarse de una Sittlichkeit adjudicada como estereotipia que se contrasta, no con otra estereotipia simétrica, sino con lo universal, que se solapa con el genérico masculino. La autora, por último, nos recuerda que la fabricación de este genio que, a título de colectivo, se nos atribuye a las mujeres tiene lugar a costa de la usurpación de nuestra individualidad y de la ausencia de esquemas pragmáticos de reconocimiento de la genialidad individual que pueda producirse en el genérico femenino. Ahora bien: es esta última la que las mujeres necesitamos para poder romper con las tradiciones establecidas e instituir nuevos relatos.

  1. Sobre o campo da Psicologia Social

    Directory of Open Access Journals (Sweden)

    Belinda Mandelbaum

    2012-03-01

    Full Text Available O campo da Psicologia Social é apresentado como território fértil, na contemporaneidade, para constituir-se como um laboratório para a produção em Ciências Humanas, uma vez que, no século XX, cada vez mais o social foi em direção ao psicológico. Neste campo, nosso embate dá-se no modo como entendemos o hífen pressuposto na integração psicossocial. A autora propõe que, entre o psicológico e o social, o hífen domina, pois ele é a própria essência relacional que é inerente a cada um dos elementos. Sugere que o modelo para entender o homem e suas circunstâncias proposto por Freud imbrica de forma indissociável o psicológico e o social, a ontogênese e a filogênese, com uma potência que teve impacto sobre todo o campo das Ciências Humanas. A Psicanálise é um instrumento hermenêutico para colaborar na elucidação dos fenômenos sociais. A autora utiliza imagens construídas por Freud e Walter Benjamin e poemas de Carlos Drummond de Andrade para fortalecer o entendimento do hífen psicossocial tanto em sua ação multidimensional quanto em sua organização.

  2. Sobre o Classicismo de Goethe (Caracter

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    Júlio César Mioto

    2009-10-01

    Full Text Available Este artigo mostra certo descompasso entre as orientações positivas da estética de Goethe, como aparecem nos seus Escritos sobre arte, e a realização efetiva de sua obra artística, tomando o caso exemplar de Ifigênia em Táuris. Tal ocorre, conforme sustentamos, por uma exigência interna à obra, que de algum modo já era compreendida por sua estética anterior. Pelo tratamento do problema clássico do “caracter”, veremos como se realiza sua concepção do procedimento maneira. Também como se dá uma superação em relação às prescrições clássicas por meio de uma particular resolução do drama.

  3. Construcciones en yeso proyectado sobre vegetales

    Directory of Open Access Journals (Sweden)

    Martínez, Hubert

    1986-07-01

    Full Text Available A construction method developed in early 1981 in Senegal, whose main objective is the evaluation of natural and human sources, specially those of wild vegetables and the phosphogypsum (a waste of chemical industry in Senegal. The introduction of the mechanical projection allows a noticeably growth in work performances and an improvement of the quality. The projection reduces de number of trades intervening in the work, doing away with plasters, watertightness systems and paintings, and simplifying the setting of technical installations and of carpentry. The acquisition of this projecting material is not very expensive, so, it is easy for small and medium enterprises to reach an expertness in gypsum work. Its finantial rentability is assured for a prize of the gypsum of 180% of the prize of the cement.Procedimiento de construcción desarrollado al comienzo de 1981 en el Senegal, teniendo como objetivo la valoración de las fuentes naturales y humanas, especialmente los vegetales en estado silvestre y el fosfoyeso, residuo de la industria química del Senegal. La introducción de la proyección mecánica permite aumentar sensiblemente el rendimiento del trabajo y mejorar la calidad. La proyección reduce el número de oficios que intervienen en la obra por la supresión de enlucidos, sistemas de estanquidad y pinturas sobre la gruesa obra y simplifica la colocación de las instalaciones técnicas y de carpintería. La adquisición del material de proyección, necesitando un capital relativamente modesto, se encuentra al alcance de pequeñas y medianas empresas que deseen especializarse en los trabajos de yeso. La rentabilidad financiera del procedimiento está asegurada para un precio del yeso de hasta el 180% del precio del cemento.

  4. Sobre el estado evolutivo de β Pictoris

    Science.gov (United States)

    Brunini, A.; Benvenuto, O. G.

    Desde el descubrimiento de fuertes excesos infrarrojos en β Pictoris, esta estrella ha sido muy estudiada y es considerada candidata a poseer un sistema planetario propio. β Pic está rodeada de un disco asimétrico de polvo que se observa de canto y que esta vacío a distancias <= 40 AU. Esto se considera una fuerte evidencia en favor de la presencia de (al menos) un planeta gigante. Recientemente se han observado líneas de material circunestelar que se han interpretado como consecuencia de la caída de objetos cometarios sobre esta estrella. Recientemente se ha utilizado la existencia del disco de polvo para atribuir una edad corta (pre - secuencia principal) a βPic. Sin embargo, la evaporación de estos cometas provee suficiente polvo como para explicar la presencia del disco observado sin necesidad de edades cortas. En este trabajo mostramos que la comparación entre la tasa de impactos cometarios estimada en el Sistema Solar para diferentes etapas de su evolución y los datos observados en β Pic indica edades avanzadas para β Pic. Esta estimación debe tomarse con cautela ya que depende de la estructura de los sistemas planetarios. Además mostramos que, desde el punto de vista de la evolución estelar y con las incertezas presentes en la luminosidad y la temperatura efectiva, existe un continuo de edades posible para β Pic. Sin embargo, empleando los datos provenientes de los flujos cometarios encontramos que una edad prolongada es consistente con ambos tratamientos.

  5. Caminando sobre huevos: Una reflexión construccionista sobre la investigación

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    Mary Jane Spink

    2006-05-01

    Full Text Available ¿Cuál es la noción de acción que guía a la investigación construccionista? Tomando esta pregunta como base, se recurre a una investigación realizada para evaluar el papel de la Comisión Nacional de SIDA (CNAIDS del Ministerio de Salud de Brasil como punto de partida para formular un concepto de acción compatible con la postura construccionista. Se comienza problematizando la noción clásica de acción, pasando a continuación a proponer alternativas, explorando las implicaciones de nociones tales como la de en-acción, discutida por Varela, para la investigación construccionista. La investigación sobre la CNAIDS se usa como ejemplo de las maneras posibles de conducir la investigación-intervención. La expresión investigación-intervención se usa en este artículo como estrategia discursiva para enfatizar que toda investigación tiene consecuencias, siendo necesariamente un proceso de producción de sentidos sobre los actos de nuestras vidas.

  6. PROTOCOLO DE KYOTO: DEBATE SOBRE AMBIENTE Y DESARROLLO EN LAS DISCUSIONES SOBRE CAMBIO CLIMÁTICO

    Directory of Open Access Journals (Sweden)

    Rodríguez S. Liliana

    2008-07-01

    Full Text Available El comportamiento climático del planeta y sus consecuencias han propiciado debates sobre los modelos de desarrollo de los países responsables del deterioro acelerado del ambiente y de los fenómenos naturales por estos días recurrentes. Sin embargo, países como Estados Unidos, luego de firmar compromisos como la Convención sobre Cambio Climático, rehúsan adquirir las obligaciones del Protocolo de Kyoto, por temor a sufrir deterioro en sus economías. En este escenario, donde la nación responsable de la emisión de aproximadamente 36% de los gases efecto invernadero resultado de la acción humana en el planeta no se compromete a adoptar políticas restrictivas tendientes a hacer sus modelos de producción más amigables con la naturaleza a pesar de que dichas medidas empiezan a ser impuestas a otras naciones como condicionantes en las negociaciones comerciales internacionales, parece ser que la adopción del Protocolo de Kyoto para países en desarrollo como Colombia no es del todo benéfica.

  7. Estudos sobre a Esquistosomose em Pernambuco, Brasil

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    Aggeu Magalhães

    1940-01-01

    Full Text Available Os autores referem os resultados dos estudos sobre esquistosomose realisados, em Pernambuco, de Novembro de 1938 a Dezembro de 1939. Não se extendem em discussão, nem comentarios, tampouco, relacionam suas verificações com o que já foi relatado sobre o assunto em trabalhos nacionais e estrangeiros, pelo fato de que, não se trata de um resultado definitivo e sim de atividades que irão proseguir. Os estudos sistematicos em torno da endemia esquistosomica foram executados em duas localidades diversas pela população e pela situação geografica e ecologica. Pontezinha é uma povoação de 1200 habitantes localizada proximo a um conjunto de charcos e lagõas de agua dôce onde pululam caramujos do tipo olivaceus de Spix, e apresenta uma taxa de infestação de 21,4% para individuos do sexo masculino, e de 14,7% para os do sexo feminino. A distribuição por grupos de idade mostra que a incidencia cresce até o grupo de 16 a 20 anos de idade, quando atinge a percentagem de 37,8%, para decrescer em seguida. Em Vitoria a incidencia foi maior nos indivíduos do sexo feminino, atingindo a taxa de 41,4%, dando o sexo masculino a taxa de 36,9%. Por idade a percentagem maxima é atingida pelo grupo de 11 a 15 anos. Vitoria é uma cidade de 1500 habitantes, construida á margem do rio Tapacurá, onde se encontram numerosos caramujos do tipo centimetralis Lutz. A divergencia de incidencia corre por conta dos habitos da população e pela utilização que ela faz do rio, sendo maior em Vitoria porque a proximidade do rio facilita o uso de suas aguas para banho e serviços domesticos, condicionando este ultimo fato, a maior infestação das mulheres. O estudo da frequencia da infestação dos caramujos por cercarias de diversos trematodios, permite suspeitar uma relação inversa entre a taxa de infestação e o diametro maximo atingido pelos caramujos, parecendo este fato confirmar os trabalhos de Vianna Martins sobre a identidade dos hospedeiros

  8. Marginalia: algumas notas adicionais sobre o dom

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    Pierre Bourdieu

    1996-10-01

    Full Text Available Prosseguindo a análise do dom, este artigo busca ultrapassar as visões fenomenológica, estruturalista e economicista do fenômeno. Para tanto, desloca o eixo de interrogação na direção do tempo e da incerteza ligados ao intervalo entre o dom e o contradom, do agente e da ação ligados às práticas, da economia de bens simbólicos e o habitus específico que a funda. Demonstrase assim, contra todo reducionismo (à consciência, ao cálculo inconsciente ou ao cálculo propriamente dito, que a razão do dom só pode repousar sobre sua dupla verdade, seu caráter generoso e obrigatório. Por outro lado, demonstrase também que foi somente através de uma revolução simbólica, e não o desenvolvimento de uma suposta natureza humana, que uma economia da troca pôde, historicamente, se destacar da economia do dom, cuja possibilidade continua aberta, dependendo apenas dos investimentos coletivos que sejam efetuados em sua direção.Resuming an analysis of the gift, this article seeks to go beyond the phenomenological, structuralist, and economicist view of this phenomenon. It thereby shifts the trust of questioning towards the time and uncertainty linked with the interval between the gift and the counter-gift, the agent and the action linked to such practices, the economy of symbolic goods, and the specific habitus underlying it. It thus demonstrates against any and all reductionism (to consciousness, to unconcious calculation, and to calculation per se that the gift's reason can only lie in its double truth, its generous and obligatory nature, both disinterested and interested. The author also demonstrates historically that it is only through a symbolic revolution, and not the development of a supposed human nature, that an economy of exchange can stand out from the economy of the gift, whose possibility remains open, requiring collective investments in its direction.

  9. As vicissitudes do "ensaio sobre o dom"

    Directory of Open Access Journals (Sweden)

    Lygia Sigaud

    1999-10-01

    Full Text Available A partir dos anos 60, antropólogos de diferentes tradições nacionais passaram a convergir no sentido de considerar que o texto de Marcel Mauss, "Ensaio sobre o Dom", contivesse uma teoria da troca, que atribui à identificação entre a coisa dada e o espírito do doador o princípio de explicação das transações. Esta interpretação contrasta com a que tiveram os contemporâneos de Mauss, os quais resgataram do trabalho suas dimensões relativas ao direito, às obrigações e às prestações totais. Partindo desta constatação, o artigo problematiza o destino do ensaio e busca torná-lo inteligível. Para tanto examina as condições sociointelectuais que contribuíram para que se produzisse a inflexão na leitura e a consolidação de uma espécie de crença coletiva em relação ao trabalho. Através deste estudo de caso, a autora identifica mecanismos que operam no processo de construção de teorias, na conformação de representações acerca de textos e na sua consagração no âmbito da antropologia.From the 1960s, anthropologists of different national traditions came to share the view that Marcel Mauss’s text, "Essay on the Gift," contained a theory of exchange which takes the identification between the thing given and the spirit of the donor as the explanatory principle of transactions. This interpretation contrasts with that held by Mauss’s contemporaries, who lifted from the work aspects relating to law, obligations and total prestations. Starting from this fact, the article problematizes the essay’s fate and seeks to make it intelligible. To this end, it examines the socio-intellectual conditions which contributed to producing this inflection in the readings of the work and the consolidation of a kind of collective belief concerning it. Through this case study, the author identifies mechanisms which operate in the process of constructing theories, in the conformity found in representations of certain texts, and in the

  10. Sobre la investigación en arquitectura y urbanismo. IV Jornadas Internacionales sobre Investigación en Arquitectura y Urbanismo.

    OpenAIRE

    Torres Cueco, Jorge

    2012-01-01

    Presentación inaugural de las IV Jornadas Internacionales sobre Investigación en Arquitectura y Urbanismo, a cargo del director de las jornadas, Sr. Jorge Torres Cueco. Torres Cueco, J. (2012). Sobre la investigación en arquitectura y urbanismo. IV Jornadas Internacionales sobre Investigación en Arquitectura y Urbanismo. 4IAU 4ª Jornadas Internacionales sobre Investigación en Arquitectura y Urbanismo. http://hdl.handle.net/10251/15033.

  11. Control de la calidad del diagnóstico coproparasitológico en la provincia de Ciudad de La Habana, Cuba External quality assessment in coproparasitology in Havana City Province, Cuba

    Directory of Open Access Journals (Sweden)

    Fidel Angel Núñez

    1997-01-01

    Full Text Available Se realizó un estudio sobre la calidad del diagnóstico coproparasitológico en 77 laboratorios de la red de salud pública de la provincia Ciudad de La Habana, Cuba. El procedimiento se basó en la entrega a cada jefe de laboratorio de un modelo de encuesta, y una bolsa de nylon conteniendo 10 viales plásticos con distintos especímenes parasitarios, preservados en formaldehído al 7%. Recogidos los resultados en las primeras 72 horas después de su entrega, se realizó la evaluación mediante una escala de puntuación establecida. La mayoría de los laboratorios aprobaron (70%; sin embargo aún existen centros, sobre todo policlínicas, con calificaciones deficientes. Los municipios con resultados más desfavorables fueron, Lisa, Marianao y Habana del Este, alcanzándose mejores resultados en los hospitales que en las policlínicas. En el análisis de Protozooarios, el mejor diagnosticado fué Giardia lamblia, con solo un centro que erró al identificarlo. Las mayores dificultades se presentaron en Blastocystis hominis con 61% de fallas, Endolimax nana, con 24,6%, y Entamoeba histolytica, con 22%. Entre los helmintos, la mayor aprobación fué en Trichuris trichiura y los errores diagnósticos predominaron con Fasciola hepatica y Taenia sp., ambos con 66,2% de fallas. Dados los resultados obtenidos, hemos organizado una intervención educativa en la red de laboratorios de la provincia.An external quality assessment in coproparasitology was carried out in 77 laboratories from Havana City. A questionnaire and ten plastic vials with different intestinal parasites in a small nylon bag, duly sealed, were sent to each laboratory. Answers were collected during the 72 hours after delivery. Results were analyzed by means of a computer program. The majority of the laboratories (70% passed the test; the municipalities with the worst scores in the province were Lisa, Marianao, and Habana del Este. Better results were obtained among technologists working

  12. Reflexiones sobre la anorexia Reflexões sobre a anorexia Reflections about anorexy

    Directory of Open Access Journals (Sweden)

    Andrea Ferrero

    2009-03-01

    Full Text Available El propósito de este artículo es presentar algunas reflexiones sobre el trastorno anoréxico, algunas posibles explicaciones sobre el mismo, y proponer una respuesta posible desde la perspectiva psicoanalítica. Este estudio plantea el sufrimiento del sujeto como la manifestación de una relación inconsciente entre las ecuaciones afectivas y el entramado social de la persona. Se discute la particular relación que se establece entre la necesidad, la demanda y el deseo en este tipo de trastornos, y cómo el síntoma anoréxico se plantea como estrategia enferma de defensa del deseo. Se puede considerar el impacto que la presión de la cultura tiene en la manutención del síntoma anoréxico, dada la importancia del ideal de delgadez y belleza, también asociados con la obtención de mayor éxito y felicidad. Finalmente, se plantea el valor del análisis de la personalidad en relación a aspectos sociales para la consideración del trastorno anoréxico, el cual debe ser comprendido singularmente, más allá de sus manifestaciones y de la posible limitación a la estructura de la histeria.O objetivo deste artigo é apresentar algumas reflexões sobre o processo de adoecimento da anorexia e algumas possíveis explicações sobre esse processo, bem como propor uma possível resposta a partir da perspectiva psicanalítica. Este estudo estabelece o sofrimento do sujeito como a manifestação da relação inconsciente entre as equações afetivas e a trama social da pessoa. Procura-se discutir a peculiar relação entre necessidade, demanda e desejo neste tipo de transtorno, e como a anorexia é uma estratégia patológica de defesa do desejo. Pôde-se observar a influência da pressão da cultura na manutenção da anorexia, justificada pelo ideal de magreza e de beleza, que também são associadas com a possibilidade de obter maior êxito e felicidade. Por último, considera-se o valor da análise da personalidade em relação aos aspectos sociais

  13. Nota sobre Marcel Mauss e o ensaio sobre a dádiva

    Directory of Open Access Journals (Sweden)

    Lanna Marcos

    2000-01-01

    Full Text Available Este artigo analisa a obra clássica de M. Mauss, Ensaio sobre a dádiva, à luz de desenvolvimentos recentes da Antropologia. Salienta como contribuição de Mauss o entendimento da dimensão política da troca de dádivas, assim como a sugestão de sua universalidade, posteriormente demonstrada por Lévi-Strauss, constituir-se em princípio formal-abstrato, e não num fato empírico-concreto. A partir desse princípio, avalia a tese segundo a qual a dádiva é fundamento de toda sociabilidade e comunicação humanas, assim como sua presença e sua diferente institucionalização em várias sociedades analisadas por Mauss, capitalistas e não-capitalistas.

  14. Uma Nota Sobre Efeitos de Gastos Públicos Federais Sobre o Crescimento da Economia Brasileira

    Directory of Open Access Journals (Sweden)

    Soraia Santos da Silva

    2014-12-01

    Full Text Available O artigo tem o objetivo de analisar os efeitos dos gastos governamentais sobre o produto da economia brasileira ao longo do período de 1980 a 2005, identificando a contribuição de algumas categorias de gastos públicos sobre o crescimento do produto no Brasil. Para isso, foram investigadas as séries dos componentes das despesas públicas federais, em particular, tanto por grupo como por função. Os procedimentos metodológicos usados basearam-se nos testes de Dickey-Fuller Aumentado (DFA e Phillips e Perron (PP, teste de Causalidade de Granger e o método de Cointegração de Engle e Granger e Johansen. Os resultados dos testes de raiz unitária mostraram que as séries estudadas de produto e despesas públicas são I(1. A maioria dos pares de variáveis mostrou causalidade no sentido de Granger fluindo dos componentes dos gastos públicos para o produto. As exceções ficaram por conta dos gastos com defesa e segurança nacional e educação. Da mesma forma, esses tipos de despesas não se mostraram estatisticamente significantes nas estimações. Entretanto, comunicação, saúde e saneamento, energia e recursos minerais e transporte foram as categorias de gastos públicos que mostraram relevância empírica na composição das despesas públicas. As evidências mostram que essas despesas podem ser consideradas como gastos produtivos. Para uma dada quantidade de fatores privados, melhores estradas, portos, ferrovias, comunicação, saúde e saneamento implicam uma maior produtividade desses fatores e, consequentemente, elevando o produto final.

  15. Comentarios sobre la Ley de Ordenamiento Territorial Sostenible

    Directory of Open Access Journals (Sweden)

    Arturo J. Yglesias

    2014-04-01

    Full Text Available La denominada Ley de Ordenamiento Territorial Sostenible, no fue bien recibida en el medio jurídico. Tanto los profesores de las diferentes materias de Derecho de nuestra Facultad como las asociaciones profesionales y otros grupos e juristas de nuestro medio, formularon reservas de diverso calibre sobre el texto de la ley que van desde sus aspectos adjetivos como la terminología y redacción hasta cuestiones sustanciales como lo que ella dice sobre el régimen de la propiedad territorial y otras cuestiones vinculadas.Desde el discurso político vimos lanzar opiniones iracundas y alarmistas sobre las modificaciones que la ley estaría haciendo al régimen de la propiedad privada, sobre su supuesta inconstitucionalidad.Nosotros nos vamos a referir a la ley a sus antecedentes y de las críticas que se le hacían, en la parte que toca a nuestra materia, esto es en lo relativo al régimen de la propiedad y debemos decir, a vía de adelanto, que no vemos en ella nada que afecte el derecho de propiedad consagrado por la Constitución de la República, ni los principios generales que regulan la propiedad territorial conforme al Código Civil, conforme a la vieja ley de Centros Poblados, sus modificativas ni demás normas sobre la materia.

  16. Perspectivas sobre la comunicación

    Directory of Open Access Journals (Sweden)

    Naranjo Pereira, María Luisa

    2005-12-01

    Full Text Available Existen diversas teorías, enfoques y modelos de comunicación; de cada uno de ellos pueden rescatarse aspectos de gran valor para el logro de una comunicación eficaz. Este documento presenta una revisión de esos aspectos, para que se pueda comprender mejor y enriquecer uno de los procesos más importantes para el ser humano: el proceso de comunicación. Este es un ensayo en el cual se da a conocer la posición de la autora sobre el tema abordado. Se rescata el acento del enfoque humanista en la relación humana y en las etapas donde se desarrolla. A partir de la comunicación pragmática, se analizan los axiomas comunicacionales en que se fundamenta y lo que se conoce como patologías de esos axiomas. Además se revisan las principales características que distinguen la asertividad en la comunicación y las distintas formas de manipulación que la entorpecen. Del enfoque de Virginia Satir, se presentan los diversos aspectos de la comunicación, tanto funcional como disfuncional y los modelos de comunicación propuestos por esta autora. Se revisan aspectos importantes del enfoque de la comunicación educativa, tales como el control de la distancia, la voz y el cuerpo, las palabras y el situacional; así como los elementos que bloquean el proceso de comunicación y las sugerencias acerca de formas correctas de escuchar. Además, se hace referencia a la necesidad de emplear procesos educativos más holísticos y constructivistas y una comunicación-interrelación horizontal y con equidad. Each of the diverse theories, approaches, and models of communication can provide valuable elements for the attainment of effective communication. This article presents a review of those elements with the goal of providing a better understanding and enhancement of one of the most important processes for human beings: the process of communication. This essay presents the author´s position on the topic. The importance given to human relationships and the stages

  17. Caracterización del estado nutricional, enteroparasitosis y condiciones socio-ambientales de la población infanto-juvenil del partido de La Plata

    Directory of Open Access Journals (Sweden)

    Oyhenart, Evelia Edith

    2013-12-01

    Full Text Available En el presente trabajo se caracterizaron el estado nutricional, las enteroparasitosis y las condiciones socio-ambientales de niños residentes en el Casco urbano y 11 localidades del Partido de La Plata, provincia de Buenos Aires, mediante un estudio transversal. Se midieron peso y talla de 6.397 niños entre 4.0 y 13.9 años. Se calculó el índice de masa corporal y se estimó el estado nutricional utilizando NHANES III. Las variables socio-ambientales se relevaron mediante encuesta estructurada. El análisis parasitológico se realizó en 729 niños sobre muestras seriadas de materia fecal analizadas mediante la técnica de Ritchie y escobillado anal. Los resultados globales indicaron: 22.1% de exceso de peso, 8.7% de desnutrición y 68.5% de parasitosis, siendo más prevalentes Enterobius vermicularis (38.7%, Blastocystis hominis (36.1%, Giardia lamblia (19.1% y protozoos comensales (26.1%. Los geohelmintos tuvieron valores inferiores (9.3%. El partido de La Plata presenta gran heterogeneidad en el estado nutricional y las infecciones parasitarias de los niños por cuanto resultan dependientes de las características socio-ambientales. Las deficientes condiciones ambientales, el bajo nivel educativo y la falta de trabajo formal de los padres explicarían la coexistencia de parasitosis y desnutrición infantil, prevaleciendo la de tipo crónico. Por otra parte, las localidades en las que hay menor grado de cobertura de servicios urbanos básicos, pero los padres poseen mayor nivel educativo y trabajo formalizado, presentan prevalencias más elevadas de exceso de peso.

  18. Sobre la atribución a Crisipo de una teoría estoica de la responsabilidad moral

    OpenAIRE

    Salles, Ricardo

    2005-01-01

    Este artículo pone en duda la tesis de que la teoría de la responsabilidad moral descrita en Alejandro de Afrodisia, De Fato, 13, y en Nemesio de Emesa, De Natura Hominis, 35, tiene como autor a un estoico tardío, probablemente Filópator (fl. primera mitad del siglo II d. C.). Esta tesis ha sido defendida recientemente por algunos estudiosos prominentes de la historia del estoicismo, entre ellos, por Susanne Bobzien en su reciente libro Determinism and Freedom in Stoic Philosophy. Por el cont...

  19. Um ensaio etnofotográfico sobre o envelhecimento

    Directory of Open Access Journals (Sweden)

    Wilson José Alves Pedro

    2016-01-01

    Full Text Available O presente artigo propõe uma síntese reflexiva – imagética e textual sobre o tema processos de envelhecimento. Trata-se de um ensaio etnofográfico, a partir de registros do diário de campo, realizados em Portugal. A partir de um enquadre teórico sociológico propõe uma reflexão sobre a complexidade da apreensão dos processos de envelhecimento, enquanto fenômeno-objeto de estudo e seus determinantes. Aponta-se a necessidade de saberes interdisciplinares e procedimentos plurimetodológicos no âmbito dos estudos gerontológicos para compreender e agir sobre as multideterminações deste processo.

  20. Efeitos dos choques fiscais sobre o mercado de trabalho brasileiro

    Directory of Open Access Journals (Sweden)

    Diogo Baerlocher Carvalho

    2013-06-01

    Full Text Available Esse trabalho tem por objetivo entender quais os efeitos dos choques fiscais sobre o mercado de trabalho brasileiro. Em particular, utiliza se uma abordagem SVAR para avaliar esses efeitos sobre o emprego e o salário real. Os choques fiscais são identificados a partir da imposição de restrições de sinais puras sobre a relação dinâmica entre os gastos do governo, o produto real e a oferta de trabalho. As restrições são obtidas a partir de funções de resposta a impulso Bayesianas derivadas de um modelo DSGE na tradição da Nova Síntese Neoclássica. As funções de resposta a impulso obtidas a partir da abordagem SVAR mostram que choques fiscais aumentam o salário real e o emprego.

  1. Surveillance for parasites in unaccompanied minor refugees migrating to Germany in 2015

    Science.gov (United States)

    Heudorf, Ursel; Karathana, Maria; Krackhardt, Bernhard; Huber, Meike; Raupp, Peter; Zinn, Christian

    2016-01-01

    In 2015, most of the refugees arriving in Germany originated from countries with poor hygienic and sanitary conditions. Stool samples of 1,230 minor refugees unaccompanied by adults were investigated for possible parasites. Giardia lamblia was by far the most frequently detected parasite (n=165); all other parasites were considerably less frequent and encountered in the following order: Hymenolepis nana (n=23), Entamoeba histolytica (n=17), Trichuris trichiura (n=8), and Blastocystis hominis (n=1). Ascaris lumbricoides was not detected among any of the screened refugees. Considerable differences in prevalence rates in refugees originating from different countries could be observed. PMID:26958459

  2. Prevalence of intestinal parasites in a population in Eghbalieh city from Qazvin Province, Iran.

    Science.gov (United States)

    Sadeghi, H; Bakht, M; Saghafi, H; Shahsavari, T

    2015-06-01

    Intestinal parasitic infections are endemic worldwide and have been described as constituting the greatest single worldwide cause of illness and disease. The prevalence of Intestinal parasitic infections was estimated to be 5.92 %. Entamoeba coli was the most common parasite followed by Giardia lamblia and Blastocystis hominis. About 5.15 % of samples contained a single parasite and 0.76 % contained multiple parasites. In this study, the prevalence of intestinal parasites especially helminthic infections was low. The study aimed to estimate prevalence of intestinal parasites in Eghbalieh city from Qazvin Province, Iran.

  3. Presentación del primer caso humano de parasitismo por Macracanthorhynchus hirudinaceus en el Perú y breve revisión

    OpenAIRE

    María Beltrán F. de Estrada

    1997-01-01

    Se reporta un caso humano portador de Macracanthorhynchus hirudinaceus. Se trata de un varón de 45 años de edad, natural de la provincia de Chota, departamento de Cajamarca, Perú; con molestias digestivas. El diagnóstico se hizo por el hallazgo de huevos de Macracanthorhynchus hirudinaceus en las heces. Además se encontró Trichuris trichiura, Ancylostoma duodenale, Trichostrongylus sp, Blastocystis hominis y Entamoeba coli. Esta es la primera comunicación de un caso humano de M. hirudinaceus ...

  4. Surveillance for parasites in unaccompanied minor refugees migrating to Germany in 2015.

    Science.gov (United States)

    Heudorf, Ursel; Karathana, Maria; Krackhardt, Bernhard; Huber, Meike; Raupp, Peter; Zinn, Christian

    2016-01-01

    In 2015, most of the refugees arriving in Germany originated from countries with poor hygienic and sanitary conditions. Stool samples of 1,230 minor refugees unaccompanied by adults were investigated for possible parasites. Giardia lamblia was by far the most frequently detected parasite (n=165); all other parasites were considerably less frequent and encountered in the following order: Hymenolepis nana (n=23), Entamoeba histolytica (n=17), Trichuris trichiura (n=8), and Blastocystis hominis (n=1). Ascaris lumbricoides was not detected among any of the screened refugees. Considerable differences in prevalence rates in refugees originating from different countries could be observed.

  5. SOBRE LA DEFINICIÓN DE LA LEY MORAL

    OpenAIRE

    2013-01-01

    Presentamos al lector de lengua castellana una traducción del texto "Sulla definizione della legge morale", del filósofo trentino Antonio Rosmini. Se trata de una respuesta elaborada por el autor para dar cuenta de las observaciones que vertiera sobre su teoría moral el jesuita Luigi Dmowski. Este último, en su libro Institutiones philosophiae arremete contra el filósofo de Trento y su tesis de que la ley moral es una noción de la mente, con cuyo uso se realiza un juicio sobre la moralidad de...

  6. Colonialismo e nacionalismo nos escritos de Marx sobre a Irlanda

    OpenAIRE

    Rafael Afonso da Silva

    2013-01-01

    Esta pesquisa tem como objeto a reflexão de Marx sobre o tema do colonialismo conduzido por países capitalistas ou, mais concretamente, pelo país capitalista que dominava o maior império colonial à época, a Inglaterra. De modo ainda mais específico, a pesquisa concentra-se nos escritos de Marx sobre a Irlanda. Esses escritos são examinados contra o pano de fundo de um conjunto mais amplo de escritos de Marx em torno do impacto do colonialismo britânico, expondo as contínuas reavaliações e rev...

  7. La socioepistemología. Un estudio sobre su racionalidad

    OpenAIRE

    Cantoral,Ricardo; López, José Iván

    2006-01-01

    Con esta investigación se pretende atender la siguiente cuestión: ¿cuál es la racionalidad que subyace a una explicación científica que se apoya en la idea de práctica?, se aborda esta pregunta haciendo una comparación con la estructura de otras teorías, que se considera tienen una cierta estabilidad. Esto nos permitió hacer inferencias sobre la racionalidad de la Socioepistemología y desde luego pretende ser también una reflexión de fondo sobre lo que significa hacer ciencia desde esta aprox...

  8. Monografía sobre los determinantes sociales de salud

    OpenAIRE

    Domínguez Santamaría, Mónica

    2012-01-01

    Las desigualdades en salud es uno de los problemas de salud que más preocupa a los responsables de Salud Pública. Las desigualdades sociales en salud han aumentado en los últimos años. Y es previsible que con la crisis económica actual el problema se agudice. El propósito de este trabajo es hacer una revisión bibliográfica sobre los documentos más importantes en los últimos cinco años sobre determinantes sociales de salud, que son las circunstancias en que las personas viven, condicionadas po...

  9. Pregnancias : sobre cuatro lavis de Colette Deblé

    OpenAIRE

    Derrida, Jacques

    2007-01-01

    ¿Cómo citar la representación del cuerpo femenino en la Historia del Arte occidental dejándose impregnar por los patrones y modelos pictóricos masculinos pero imprimiendo, a su vez, otras huellas, las de otro cuerpo? Esta pregunta atraviesa el texto del filósofo Jacques Derrida sobre las aguadas (lavis) de la pintora francesa Colette Deblé, dando lugar a una reflexión sobre el engendramiento, el parto y la cita pictórica. En el trabajo de Colette Deblé, el legado de la Historia del Arte y su ...

  10. Apuntes sobre la noción de afectividad colectiva

    OpenAIRE

    2010-01-01

    El presente texto, que se suscribe en el paradigma de la psicología social crítica, trata la temática de la afectividad colectiva, revisando en un primer momento las nociones clásicas en psicología sobre los afectos como proceso individual para, posteriormente, esbozar la premisa sobre la afectividad como una construcción social y colectiva, noción revisada desde los planteamientos de Pablo Fernández Christlieb, quien alude a la función cultural que cumplen los afectos en el plano de la vida ...

  11. Concepciones implícitas sobre el desarrollo infantil

    OpenAIRE

    Vilar Martínez, C.; Blasco Cubedo, María Jesús; Hernández Blasi, Carlos

    2001-01-01

    Setenes Jornades de Foment de la Investigació de la FCHS (Any 2001-2002) Partiendo del trabajo de Martí, E. et al. (1994) “ Ideas previas de una población universitaria sobre el desarrollo infantil” hemos realizado un estudio descriptivo para identificar las concepciones implícitas del conocimiento que tienen estudiantes universitarios sobre el desarrollo infantil. La muestra estaba constituida por 111 estudiantes de la Universitat Jaume I, con una media de edad de 19 años. Median...

  12. Santa sexualidad: concepciones de dos monjas sobre feminidad y sexualidad

    OpenAIRE

    Isabel Cristina Giraldo Quijano

    2009-01-01

    En el contexto de la religión católica, la feminidad y la sexualidad femenina se configuran según dogmas y representaciones sociales específicos, en los que la imagen de Cristo -y todo lo que ello implica- actúa como elemento fundamental en la construcción de las concepciones de las mujeres consagradas a la vida religiosa sobre sex ualidad, erotismo, cuerpo. amor. Este artículo pretende explorar desde un punto de vista sociológico las concepciones de dos monjas sobre los temas mencionados....

  13. Efectos del consumo moderado de cerveza sobre la salud cardiovascular

    OpenAIRE

    Sendino Sedano, Pablo José

    2015-01-01

    La cerveza es una bebida fermentada elaborada a partir de cebada malteada. Entre sus componentes se encuentran el etanol (excepto en la cerveza sin alcohol) y los polifenoles, ambos con numerosos efectos sobre la salud humana, y en especial sobre la salud cardiovascular. Así mismo la práctica de ejercicio de forma regular es un hábito saludable. El sistema cardiovascular tiene numerosas funciones muy importantes en el organismo, y también mucha patología relacionada. Uno de los cuadros clínic...

  14. A sangre y fuego: Nuevos datos sobre la metalurgia Aguada

    OpenAIRE

    2002-01-01

    Las placas de metal figuran entre los ítemes más característicos de la cultura material de Aguada. Se conocen poco más de 30 ejemplares, los cuales se encuentran dispersos en colecciones de América y Europa y muy pocos de ellos fueron sometidos a análisis técnicos. En esta oportunidad se dan a conocer los resultados de los estudios realizados sobre tres placas Aguada, orientados a obtener información sobre las cualidades tecnológicas de los materiales, aplicable al conocimiento de la dinámica...

  15. EL IMPUESTO SOBRE BEBIDAS AZUCARADAS EN ESPAÑA

    OpenAIRE

    Vicente Ortún; López-Valcárcel, Beatriz G; Jaime Pinilla

    2016-01-01

    Este artículo aporta una revisión crítica acerca de los retos a los que se enfrentan los impuestos sobre las bebidas azucaradas como instrumento de políticas de salud, para revertir la tendencia epidémica de la obesidad. Se valoran las experiencias de los países más significados, en particular México, y se reflexiona sobre el contrapeso que ejerce la industria a las políticas antiobesidad y el poder de los lobbies. Esas políticas impositivas en pro de la salud pública han de sobreponerse a la...

  16. Nuevos documentos sobre Rojas Zorrilla y su teatro

    Directory of Open Access Journals (Sweden)

    Madroñal, Abraham

    2007-06-01

    Full Text Available This article contains twelve unpublished documents on Rojas Zorrilla, related to his biography and literary works (comedias and autos sacramentales. The majority are handwritten by the poet himself, and they provide new information relevant to our knowledge of his life and to the understanding of his works.Se publican una docena de documentos inéditos sobre Rojas Zorrilla referidos a su biografía y a algunas comedias y autos. En buena parte dichos documentos son autógrafos del poeta y añaden o matizan informaciones concretas sobre él y sus obras.

  17. EL PENSAMIENTO SOCIAL SOBRE EL SUICIDIO EN ESTUDIANTES DE BACHILLERATO

    OpenAIRE

    Gabriel Montes Sosa; Francisco Montes Villegas

    2009-01-01

    En este estudio se analiza desde el marco de las representaciones sociales cómo los jóvenes preparatorianos manifiestan su pensamiento social sobre el suicidio. Se aplicaron cuestionarios abiertos a 169 jóvenes, mujeres y varones de bachillerato, para conocer la ocurrencia potencial de suicidio, las situaciones a las que se debe y su definición. Los resultados muestran que los jóvenes organizan su idea sobre el suicidio a partir de varios elementos en común: problemas diversos, depresión, baj...

  18. Santa sexualidad: concepciones de dos monjas sobre feminidad y sexualidad

    Directory of Open Access Journals (Sweden)

    Isabel Cristina Giraldo Quijano

    2009-12-01

    Full Text Available En el contexto de la religión católica, la feminidad y la sexualidad femenina se configuran según dogmas y representaciones sociales específicos, en los que la imagen de Cristo -y todo lo que ello implica- actúa como elemento fundamental en la construcción de las concepciones de las mujeres consagradas a la vida religiosa sobre sex ualidad, erotismo, cuerpo. amor. Este artículo pretende explorar desde un punto de vista sociológico las concepciones de dos monjas sobre los temas mencionados.

  19. Optimización sistólica sobre GPUs

    OpenAIRE

    Vidal, Pablo; Alba Torres, Enrique; Luna, Francisco

    2012-01-01

    En este trabajo se estudia una nueva aproximación algorítmica basada en un modelo de procesamiento paralelo conocido como computación sistólica. Este algoritmo se ejecuta sobre una Unidad de Procesamiento Gráfico empelando CUDA (Compute Unified Device Architecture). El algoritmo se compone de una matriz de celdas, donde cada una de ellas trabaja sobre una solución, realizando operaciones que la modifican para luego moverla a la celda contigua y repetir el proceso. Hemos evaluado el comportami...

  20. Concurrencia de titulares sobre la creación intelectual

    OpenAIRE

    Navarro Costa, Ruth

    1994-01-01

    El contenido de la tesis presentada bajo el titulo "Concurrencia de titulares sobre la creación intelectual" comprende un estudio pormenorizado de las obras debidas a un sujeto plurimo. Así, los capítulos i, ii y iii tratan respectivamente de las obras en colaboración, colectivas y derivadas como supuestos mas emblemáticos de cotitularidad sobre la obra del ingenio. Finalmente, en el capitulo iv se abordan algunos supuestos heterogéneos que pueden dar lugar a situaciones de concurrencia entre...

  1. Datos sobre la vida sexual en dos ciudades colombianas

    OpenAIRE

    2012-01-01

    Uno de los temas más difíciles de investigar científicamente es el referente a la vida sexual, principalmente por la dificultad de obtener datos precisos y verídicos sobre la conducta de los individuos. Este es un tema que se trata vagamente ó solo a través de la consulta médica ó el chiste. Resulta difícil preguntar a la gente sobre su conducta sexual y más difícil aun obtener una respuesta verdadera y clara. Este informe, aunque breve, ha logrado en parte eludir estos escollos. En primer lu...

  2. Comentarios al Trabajo del Académico Dr. Ernesto Bustamante Z. sobre “Agresión”

    Directory of Open Access Journals (Sweden)

    Efraim Otero Ruiz

    1992-06-01

    Full Text Available

    Señores académicos, señoras, señores:

    Quiero ante todo congratular al académico Bustamante Zuleta por esta nueva incursión en el tema de la agresión, tema que, por la misma situación convulsionada que vive el país, ha sido de gran interés para esta Academia en los últimos lustros. Esta vez hemos oído las opiniones de alguien que, aunque proveniente de la neurología y la neurocirugía, es además un reflexivo filósofo y un acertado analista del comportamiento humano.

    Por eso no es de extrañar que, en la primera parte de su trabajo, se dedique a analizar los móviles de la agresi6n y de la violencia humanas, yendo desde las culturas más primitivas, cuando el hombre se convirti6 en predador de sus semejantes o en el “horno homini lupus” de Hobbes, hasta la conducta agresiva y la violencia que vivimos en nuestros días y en que los m6viles pueden ser políticos, econ6micos, sociales o religiosos, acompañados de lo que él denomina la “regresión social” en el sentido que le da Elliot en su tratado de Neuropsiquiatria.

    Uno de los problemas que tiene que abocar quien enfrenta estos temas es la definición misma del término “agresión” o “conducta agresiva”: ya en su presentación hace un año en esta Academia el psicobiologo sevillano Jesús Martín Ramírez nos señalaba la diferencia entre idiomas como el inglés y el español y la cantidad de definiciones que habían hecho decir a Johnson, que “el término agresi6n lo han hecho significar tantas cosas que, en realidad, lo que han hecho es hacerle perder su significado”. Infortunadamente el trabajo del Dr. Martín Ramírez (que esperamos sea publicado muy pronto en el volumen de “Temas Médicos” pr6ximo a aparecer era tan extenso y escrito en un lenguaje tan técnico que mucho de él se perdi6 al tratar el autor de resumirlo para su presentaci6n en esta Academia.

    De todas maneras, como lo podrá comprobar quien lo lea despacio, su

  3. Controversia sobre el origen del anime. Una nueva perspectiva sobre el primer dibujo animado japonés

    OpenAIRE

    2012-01-01

    Recientes descubrimientos sobre los orígenes de la animación japonesa muestran indicios consistentes de que la primera animación nipona en formato cinematográfico data de la década de 1900, lo que puede llegar a cuestionar algunos aspectos fundamentales que sustentan la historia de la animación tradicional. En este artículo se realiza un análisis de la documentación existente sobre las primeras animaciones japonesas, demostrando que este peculiar estilo de animación tuvo en realidad un origen...

  4. Construcciones sociales sobre mujeres desde el discurso jurídico en sentencias penales sobre infanticidio

    Directory of Open Access Journals (Sweden)

    María Eugenia Gastiazoro

    2015-12-01

    Full Text Available El infanticidio como figura penal se suprime del Código Penal Argentino en 1994, para ser una figura de homicidio agravado por el vínculo con prisión o reclusión perpetua. Diez años después de su última derogación, el caso Romina Tejerina en Jujuy generó una serie de cuestionamientos respecto de su penalización. Otro caso, que tomó estado público fue el de Eli Díaz en la ciudad de Villa Dolores en Córdoba, juzgada en el 2006, siendo (en contraposición al caso Tejerina absuelta por una mayoría compuesta solo de ciudadanos comunes (jurados. En el presente trabajo se analiza la construcción y la producción que desde el discurso jurídico se hace de las diferentes mujeres en los casos de infanticidio. A su vez, estas imágenes se comparan con las representaciones que los legisladores tuvieron en el debate del Congreso del año 2010 cuando se intentó reponer la figura. La sujeción del género centrada en la buena o mala madre, mujer, esposa, en intersección con otras dimensiones de clase social, y edad, se categoriza en el discurso de los tribunales de acuerdo a imaginarios sociales que sostienen una identidad normativa sobre estas mujeres.

  5. SOBRE LA PAZ Y LOS DIÁLOGOS. CONSIDERACIONES SOBRE EL PAPEL DE LA SOCIEDAD CIVIL

    Directory of Open Access Journals (Sweden)

    William Ortiz Jiménez

    2014-10-01

    Full Text Available El ensayo hace una reflexión en torno al proceso de paz que se llevó a cabo en el gobierno del presidente Andrés Pastrana, y la participación que tuvo la sociedad civil en este asunto. Pero, trasciende, además, a la situación actual del conflicto en Colombia. A partir de las formulaciones de varios teóricos, entre los que se destacan Daniel Pécaut, Cohen, y Arato., Margarita Bonamusa, María Teresa Uribe, Daniel García-Peña, Habermas, Rawls, entre otros, se fortalece un marco teórico y de referencia bastante sólido para mostrar cómo lo público es indispensable en las acotaciones que lleven a la solución de los conflictos. Sin el concurso de lo público, tampoco sería viable la participación de la sociedad civil. Los espacios de solución de conflictos, el ágora, en palabras de Hanna Arendt, es el lugar esencial para la discusión, el consenso y los acuerdos. En política, la esfera de lo público prima sobre lo privado, porque el reconocimiento de las libertades, manifestaciones y la plenitud de los derechos sólo se logra en lugares comunes.

  6. Pensamiento escolástico actual sobre el evolucionismo

    Directory of Open Access Journals (Sweden)

    Jorge Enrique Leal G.

    1962-07-01

    A este propósito nos ha parecido no solo conveniente, sino quizá útil, ensayar un recuento así sea somero y sintético, sobre las ideas que en la actualidad abriga la escuela escolástica en sus principales matices.

  7. Aspectos sobre el matrimonio en el Derecho Romano arcaico

    OpenAIRE

    Fern??ndez Baquero, Mar??a-Eva

    2004-01-01

    El libro "El Derecho de Familia: De Roma al Derecho actual" donde se encuentra recogido este cap??tulo "Aspectos sobre el matrimonio en el Derecho Romano arcaico" (p??gs. 193-207) tiene un total de 853 p??ginas.

  8. Sobre-educación en el mercado laboral colombiano

    Directory of Open Access Journals (Sweden)

    Jhon James Mora

    2008-12-01

    Full Text Available Este artículo analiza la sobre-educación en Colombia con datos de individuos y empresas compilados por el Servicio Nacional de Aprendizaje de Colombia en 2006, encuentra que la probabilidad de estar sobre-educado es del 14% y que depende de la movilidad entre áreas de desempeño, la experiencia, el género, el tamaño de la empresa y el nivel de educación. Los trabajadores sobre-educados ganan un 2% menos que los trabajadores correctamente educados, conforme al patrón internacional. Los resultados también muestran que la probabilidad de que los profesionales permanezcan en la misma área de desempeño aumenta con la experiencia, que cuanto mayor es la sobre-educación menor es la probabilidad de permanecer en dicha área y que los trabajadores con postgrado tienen menor probabilidad de permanecer en su área de desempeño.

  9. Sobre o estilo e o modo de falar e escrever

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    Pedro Caruso

    2001-02-01

    Full Text Available

    Este trabalho apresenta o texto crítico de uma Lei ou Provisão de Filipe I , rei de Portugal, sobre as diversas formas de tratamento no período arcaico da língua portuguesa.

  10. Indagación sobre la visión

    OpenAIRE

    Solbes Matarredona, Jordi

    2011-01-01

    Indagación sobre la luz y la visión usando objetos de la vida cotidiana: camara oscura y su fabricación con tubos de cartón, espejos planos, concavos y convexos (cucharas pulidas), lentes convergentes y divergentes, funcionamiento del ojo, etc.

  11. Intestinal parasitic infections in suburban government schools, Lak Hok subdistrict, Muang Pathum Thani, Thailand

    Institute of Scientific and Technical Information of China (English)

    Sirima Kitvatanachai; Pochong Rhongbutsri

    2013-01-01

    Objective:To provide baseline information of parasitic infections in 3 suburban government schools, Lakhok subdistrict of Muang Pathum Thani, Thailand. Methods: This study was conducted between May-June 2010 using simple direct smear and modified formalin ether and from a population of 1 253 in 3 suburban government schools. Results: Total samples of 202 registered and participated. The average of prevalence of infection from 3 schools was 13.9%, there were 13.7%, 14.3%and 13.9%in N, S and R school, respectively with no significant difference between schools (P>0.05). The infection rates did not show significant difference between genders (P>0.05). The highest rate of infection was 20.4%in Pathom 2 (8 years) students and the lowest was 4%in Pathom 1 (7 years) with statistically difference between age groups (P<0.05). The highest prevalence of pathogenic protozoa was Giardia lamblia (G. lamblia) which was found in 50%of infected cases, followed by 25%of Entamoeba histolytica (E. histolytica) and Blastocystis hominis (B. hominis). The highest prevalence of non-pathogenic protozoa was Endolimax nana (E. nana) which was found in 88.9%, followed by 11.1%of Entamoeba coli (E. coli). Mixed infections between Blastocystis hominis (B. hominis) and Endolimax nana (E. nana) were reported at 7.1%. The only helminthic infection found in this study area was hookworm, found in 1 student (3.8%). The formalin ether concentration technique showed a higher efficacy of detection (78%-100%) than the simple direct smear method (0%-50%). Conclusions: Surveillance of Protozoan infections may need to be focused on suburban areas.

  12. Campylobacter hominis sp nov., from the human gastrointestinal tract

    DEFF Research Database (Denmark)

    Lawson, A.J.; On, Stephen L.W.; Logan, J.M.J.

    2001-01-01

    , an isolation strategy was developed employing initial non-selective membrane filtration onto fastidious anaerobe agar. Campylobacters were then isolated from the resulting mixed microbial flora by a dilution strategy and/or by immunomagnetic separation with genus-specific polyclonal antibody. Isolates were...

  13. Analysis of a Mycoplasma hominis membrane protein, P120

    DEFF Research Database (Denmark)

    Christiansen, Gunna; Mathiesen, SL; Nyvold, Charlotte Guldborg;

    1994-01-01

    extension analysis. The gene contained an open reading frame of 3237 bp encoding a peptide of 1079 amino acids with a deduced molecular mass of 123 kDa. A putative amino-terminal signal peptide and cleavage site for signal peptidase II were found. This suggests that the protein was synthesized...

  14. "Meu prazer agora é risco": sentidos sobre sexualidade entre jovens de um grupo sobre saúde

    Directory of Open Access Journals (Sweden)

    João Paulo Pereira Barros

    2013-04-01

    Full Text Available A sexualidade assumiu a condição de centralidade subjetiva pelo entrecruzamento de diversos demarcadores da Modernidade, como a legitimação do saber-verdade da ciência, a afirmação da individualidade e a institucionalização dos segmentos infanto-juvenis. Partindo dessa premissa, este artigo analisa sentidos produzidos por jovens sobre "sexualidade" em um grupo de discussão sobre saúde, com base em uma pesquisa-intervenção operacionalizada por observações-participantes e pela formação de oficinas sobre saúde, em uma escola pública de Fortaleza. Acerca dos sentidos sobre "sexualidade", as articulações entre "sexualidade" e "risco" se sobressaíram em relação às conexões entre "sexualidade" e "prazer". Isso sugere a força de um viés educativo baseado na scientia sexualis, aludido por Michael Foucault, operando com uma visão normativa-prescritiva da sexualidade, reiterando-a, mediante o signo da "prevenção", como tecnologia de governo. Usando como ferramenta o conceito de biopolítica, aponta-se que tais questões remetem à instalação de um paradigma de "gestão do risco" na contemporaneidade.

  15. Narrativas sobre síndrome de Down no Festival Internacional de Filmes sobre Deficiência Assim Vivemos

    Directory of Open Access Journals (Sweden)

    Ana Cristina Bohrer Gilbert

    Full Text Available O Festival Internacional de Filmes sobre Deficiência Assim Vivemos acontece bienalmente no Brasil desde 2003. Busca engajar a audiência em novas perspectivas sobre deficiência por meio de filmes, atividades interativas, imagens e discursos institucionais. Neste artigo, analisam-se os filmes cujo tema central é a síndrome de Down, exibidos entre 2003 e 2013, com o objetivo de examinar o tipo de construção narrativa e as imagens e os significados sobre pessoas com essa condição que são veiculados no festival. Foram utilizadas estratégias etnográficas no tratamento do material empírico e procedimentos semióticos de leitura isotópica na sua análise. Foram identificados três núcleos narrativos principais que acompanham o desenvolvimento teórico sobre deficiência, sendo que um deles apresenta-se alinhado com o que tem sido denominado de ‘estética da deficiência’.

  16. Centros de informação sobre medicamentos no Brasil : passado, presente e perspectivas do Sistema Brasileiro de Informação sobre Medicamentos

    OpenAIRE

    Carlos Cezar Flores Vidotti

    1999-01-01

    Resumo: Introdução. Após discussão dos diferentes fatores que justificam aexistência de Centros de Informação sobre Medicamentos (CIM), foram apresentadas as definições de informação sobre medicamentos (IM)e de centro de informação sobre medicamentos (CIM), e relacionadas às atividades usuais dos centros, tendo a informação passiva como atividade fundamental. O farmacêutico especialista em informação sobre medicamentos é o profissional com perfil mais adequado para atuar nos CIM, devendo ser ...

  17. Sobre a Pesquisa nas Artes: um discurso amoroso

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    Victoria Pérez Royo

    2015-09-01

    Full Text Available Este artigo resulta de um exercício de reescrita experimental e de um pensamento analógico cujo objetivo era abrir uma nova perspectiva sobre pesquisa nas artes. Metodologicamente, as diferentes figuras de amor no livro Fragmentos de um Discurso Amoroso, de Barthes, são levadas a conversar sobre a relação entre pesquisador e objeto de estudo. Essa analogia nos permite encontrar parâmetros de qualidade baseados em uma escala de valores diferentes dos hegemônicos na academia (produtividade, competitividade, inovação. Esses novos parâmetros podem constituir uma sólida base ontológica para a construção de uma nova política de pesquisa artística na academia que permita uma reconsideração radical dos processos de investigação em artes.

  18. ensayo crítico sobre Oliverio Girondo

    Directory of Open Access Journals (Sweden)

    Luis Felipe González G.

    2005-01-01

    Full Text Available A partir de la aparición, por cerca de diez años, del movimiento surrealista, se hará un esbozo de algunas las características de uno de los representantes de esta escuela literaria, Oliverio Girondo, partiendo de un poema del libro Veinte poemas para ser leídos en el tranvía, del cual se encontraron dos posiciones, en apariencia antagónicas, sobre la vanguardia, derivadas en un antiestilo o decadencia de los principios modernos (Calinescu, 1991 y una posibilidad de crítica a la institución arte (Bürger, 1987. Estas posiciones permitieron establecer algunas reflexiones acerca del concepto de saturación social que postula el construccionismo social y las características generales que propone Mukarovsky (1992 sobre la teoría crítica estructuralista.

  19. MEMORIA EMOCIONAL: EFECTOS DE LA CORTICOSTERONA SOBRE LOS RECUERDOS

    Directory of Open Access Journals (Sweden)

    ELIANA RUETTI

    2008-01-01

    Full Text Available Las hormonas adrenales, administradas inmediatamente antes o después de una tarea de aprendizaje, modulan la memoria. Este efecto varía de acuerdo al tiempo y a la intensidad con que se incrementan las hormonas. La liberación o administración de glucocorticoides inmediatamente antes o después de una tarea, facilitan la memoria. Por el contrario, la elevación o administración de glucocorticoides antes de una prueba de aprendizaje, deteriora la evocación de la información previamente adquirida. En este trabajo se revisan los principales estudios realizados con animales y humanos acerca del papel de los glucocorticoides sobre la memoria de eventos aversivos. A su vez se describe el efecto de la elevación aguda y crónica de las hormonas adrenales sobre la memoria.

  20. Instalación fotovoltaica sobre cubierta de nave industrial

    OpenAIRE

    Ramírez Basalo, Miguel Ángel

    2013-01-01

    El objetivo de este proyecto es el diseño y estudio de la instalación fotovoltaica con conexión a red sobre cubierta en una nave industrial, así como la iluminación interior y exterior de la misma nave. La instalación fotovoltaica sobre cubierta con conexión a red es una de las más demandadas hoy en día, y que puede reportar muchos beneficios y ventajas en el futuro. No es una necesidad real, pero se justificara su elección a través de un estudio técnico y económico, en el q...

  1. Bombeiros: um olhar sobre a qualidade de vida no trabalho

    Directory of Open Access Journals (Sweden)

    Janine Kieling Monteiro

    Full Text Available Este trabalho apresenta uma intervenção no corpo de bombeiros (São Leopoldo - RS realizada pelo NEPT (Núcleo de Excelência em Psicologia do Trabalho. Utilizamos um diagnóstico com sessenta e três entrevistas semi-estruturadas sobre o trabalho e funcionamento da corporação. Partindo da análise deste, propusemos palestras sobre estresse e qualidade de vida no trabalho (QVT. Foram levantados questionamentos acerca do mito do herói e do amor pela profissão bombeiro, que deixa em segundo plano seus problemas. Destacou-se o estresse no trabalho por situações traumáticas, como risco de vida eminente e mortes nos salvamentos. Entendemos que as intervenções contribuíram como espaço de escuta que propiciou reflexões a respeito da QVT.

  2. Planteamientos sobre la pobreza: una aproximación conceptual

    Directory of Open Access Journals (Sweden)

    Bertha Lucía Martínez Bernal

    2015-07-01

    Full Text Available En el presente artículo se presentan varias definiciones o conceptos para la comprensión, medición y el análisis de la pobreza. Para ello se retoma la producción escrita de diferentes estudiosos e investigadores que han hecho aportes valiosos sobre el tema. Al lado de estos conceptos se analizan los diferentes enfoques que se utilizan para aproximarse al estudio de la pobreza, aunque estas no son teorías ni explicaciones de sus causas, sino perspectivas sobre pobreza que denotan dichos enfoques. Así mismo se desarrolla una revisión de las metodologías más utilizadas en los procesos de identificación y agregación. Finalmente se interpretan las políticas públicas en los sectores sociales.

  3. EL PENSAMIENTO SOCIAL SOBRE EL SUICIDIO EN ESTUDIANTES DE BACHILLERATO

    Directory of Open Access Journals (Sweden)

    Gabriel Montes Sosa

    2009-01-01

    Full Text Available En este estudio se analiza desde el marco de las representaciones sociales cómo los jóvenes preparatorianos manifiestan su pensamiento social sobre el suicidio. Se aplicaron cuestionarios abiertos a 169 jóvenes, mujeres y varones de bachillerato, para conocer la ocurrencia potencial de suicidio, las situaciones a las que se debe y su definición. Los resultados muestran que los jóvenes organizan su idea sobre el suicidio a partir de varios elementos en común: problemas diversos, depresión, baja autoestima y comunicación deficiente. Así, el suicidio se objetiviza en estos esquemas y se ancla en sus relaciones existentes. Para contextualizar el estudio, en la primera parte del trabajo los autores incluyen una somera descripción de los datos estadísticos más importantes respecto del suicidio en el estado de Puebla.

  4. Pregnancias. Sobre cuatro lavis de Colette Deblé

    Directory of Open Access Journals (Sweden)

    Jacques Derrida

    2013-07-01

    Full Text Available ¿Cómo citar la representación del cuerpo femenino en la Historia del Arte occidental dejándose impregnar por los patrones y modelos pictóricos masculinos pero imprimiendo, a su vez, otras huellas, las de otro cuerpo? Esta pregunta atraviesa el texto del filósofo Jacques Derrida sobre las aguadas (lavis de la pintora francesa Colette Deblé, dando lugar a una reflexión sobre el engendramiento, el parto y la cita pictórica. En el trabajo de Colette Deblé, el legado de la Historia del Arte y su mitología engendrarán una serie infinita de aguadas donde el agua no pretende “lavar” la tradición (deshacerse de ella, acabar con ella sino inundarla, mojarla, empaparla con nuevas aguas para, escurriéndola, hacerla resonar nuevamente.

  5. Consenso Cultural sobre el Intento de Suicidio en Adolescentes

    Directory of Open Access Journals (Sweden)

    Javier Eduardo García de Alba García

    2011-01-01

    Full Text Available Este estudio buscó identificar la estructura semántica del dominio cultural, el promedio de conocimiento y el grado de consenso cultural manifestado por los adolescentes sobre el intento de suicidio. Fue un estudio cualitativo de listas libres, con un muestreo propositivo no aleatorizado de 27 adolescentes entre 13 y 18 años. Los resultados evidenciaron un solo modelo semántico. En lo estructural se identificó el problema familiar como una causa importante del intento suicida; las categorías depresión y tristeza se consideraron como signos y síntomas previos a esta tentativa y no como causas. Como estrategias de prevención, se consideró recibir información mediante pláticas o establecer conversaciones sobre los problemas. Este modelo permite proponer estrategias de prevención que privilegien el núcleo familiar.

  6. ANOTAÇÕES SOBRE A FOTOGRAFIA DE FUTEBOL

    Directory of Open Access Journals (Sweden)

    Rui Cezar dos Santos

    2004-12-01

    Full Text Available O autor apresenta de maneira sintética as principais evoluções na fotografia e sua apropriação pelo fotojornalismo esportivo. Discorre sobre a imaginária que a Indústria Cultural sabe explorar, produzindo rica iconografia do esporte, dentro da qual o atleta surge ao mesmo tempo como ídolo, de maneira idealizada, e como atleta, nos fragmentos que realçam o detalhe.

  7. Algunas consideraciones sobre la investigación de desarrollo

    Directory of Open Access Journals (Sweden)

    Nadal, J.

    1970-07-01

    Full Text Available Not availableAnte el auditorio más calificado de España, me invitan a desarrollar esta ponencia que trata de un tema al que habéis dedicado todos muchas horas de meditación. Un tema sobre el que cada uno de vosotros ha procurado acumular cuanta información le ha sido posible y, en definitiva, una materia de la que sois verdaderos maestros.

  8. Ensayo sobre el feminismo y el sujeto popular

    OpenAIRE

    León Nuñez, Carmen Violeta

    2012-01-01

    Se comenta acá el ensayo escrito por Ana Sojo, el cual se compone de tres partes principales  componen el texto: la primera versa  sobre  el  sujeto popular: la segunda se refiere al estudio de  la definición de la condición subalterna de la mujer y en la tercera analiza el feminismo y el ámbito de la política.

  9. Dialogos negativos anotaciones sobre el montaje de Medea Okupa

    Directory of Open Access Journals (Sweden)

    Marcelo José Islas

    2013-09-01

    Full Text Available En este texto hablamos de dos temas: la adaptación de una obra de Eurípides, ]Medea, y su montaje en el contexto de un espacio público a partir de un trabajo de investigación en actuación sobre la poética de la tragedia.Estos dos temas, que exponemos por separado una mejor comprensión.

  10. Documentos sobre la censura de libros en el siglo XVII

    Directory of Open Access Journals (Sweden)

    María Teresa Cobos

    1963-04-01

    Full Text Available Durante el siglo XVII, en materia de legislación sobre censura de impresión de libros , no se produjeron modificaciones substanciales en las directrices señaladas en la centuria precedente, especialmente en las contempladas en la Pragmática de Felipe II del 7 de septiembre de 1558 y en otros preceptos legales que la reiteraron y perfeccionaron.

  11. Una nota sobre "la transformación correcta"

    Directory of Open Access Journals (Sweden)

    Muñoz M. Alberto

    1986-12-01

    Full Text Available En la nota presente se intenta demostrar que hay algunos problemas conceptuales y lógicos en el artículo aludido, aunque presente aspectos incisivos y nuevos sobre la relación entre valores y precios, referidos al producto neto, a la composición orgánica del capital, a los salarios y a la relación plusvalía y ganancias

  12. Efectos del tipo funcional de entrenamiento sobre el ajuste lector

    OpenAIRE

    Rosalinda Arroyo Hernández; Guadalupe Mares Cárdenas

    2009-01-01

    El presente trabajo tuvo por objetivo evaluar los efectos de entrenar cinco tipos de contacto con las relaciones entre objetos y sus elaboraciones lingüísticas sobre el ajuste lector de estudiantes universitarios. Primeramente los participantes fueron expuestos a una evaluación de ajuste lector, consistente en preguntas de diferente complejidad funcional, para posteriormente ser asignados aleatoriamente a uno de cinco grupos experimentales diferenciados por el nivel de complejidad funcional d...

  13. Sobre inundaciones y anegamientos / Reflections on floods and flooding

    Directory of Open Access Journals (Sweden)

    Ferrando A., Francisco J.

    2006-11-01

    Full Text Available Respecto a anegamientos e inundaciones, el autor realiza algunas precisiones conceptuales que afectan la gestión de acciones preventivas, la planificación y el ordenamiento territorial; además se ofrece una sistematización del quehacer sobre las inundaciones./ The author punctualizes the concepts regarding preventive actions and territorial planning. Also the article includes a systematized list of actions related to flood management.

  14. Corpus de inscripciones sobre la esclavitud infantil en Hispania romana

    OpenAIRE

    Crespo Ortiz de Zárate, Santos

    2009-01-01

    Se presenta en este trabajo el corpus epigráfico relativo a la servidumbre infantil en Hispania romana, como base a una serie de estudios sobre la dependencia personal en este ámbito social. This work shows the epigraphic sources dealing with the children-slaves in Roman Spain and sets up a base for future studies about personal dependence in this social sphere.

  15. Hegel sobre Tácito y la libertad teutona

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    Valerio Rocco Lozano

    2015-09-01

    Full Text Available Una investigación sobre la presencia (así como sobre la ausencia de Tácito en Hegel es crucial para entender la posición política y filosófica de este pensador, especialmente en la época de Jena. Tras algunas consideraciones sobre el conocimiento hegeliano de la historia y la cultura romanas, se abordará un análisis de su noción de "libertad teutona": Hegel se opone tanto a la neo-romanidad de los revolucionarios franceses como al esencialismo teutómano de los conservadores alemanes (derivado fundamentalmente de un uso fuertemente ideológico de la "Germania" de Tácito, así como a las caducas instituciones imperiales. Esta doble oposición sitúa a Hegel en medio de dos corrientes políticas (e incluso de dos ejércitos en guerra y se lleva a cabo desde una actitud que puede denominarse como “mediación sin nostalgia”

  16. O Impacto do Mobbing sobre o estresse no trabalho

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    Tássia Donadello Ferreira Lima

    2015-07-01

    Full Text Available Definido como um conjunto de ações hostis e antiéticas, desenvolvidas no ambiente de trabalho de forma sistemática e recorrente que deixa o trabalhador em uma posição indefesa, o mobbing tem sido investigado na sua relação com a saúde e qualidade de vida do trabalhador. Neste sentido, o objetivo no presente trabalho foi (1 o desenvolvimento de três instrumentos para mensuração do mobbing, considerando sua prática por chefes, colegas e subordinados e (2 investigar o impacto do mobbing sobre o estresse no trabalho. Participaram do estudo 300 servidores de uma universidade federal do estado do Rio de Janeiro, sendo 157 homens e 143 mulheres. Os participantes preencheram os três instrumentos para mensuração do mobbing e a EET (Escala de Estresse no Trabalho. A análise fatorial exploratória dos principais eixos com rotação promax revelou uma estrutura ideal de dois fatores em cada instrumento, sendo nomeados de (1 comportamento respeitoso e (2 mobbing praticado por chefes, colegas ou subordinados, conforme o caso. A Modelagem por Equações Estruturais revelou um impacto positivo significativo do mobbing sobre o estresse no trabalho. Da mesma maneira, o comportamento respeitoso por parte de chefes, colegas e subordinados teve influência negativa significativa sobre o estresse no trabalho.

  17. Sobre os conceitos de risco em Luhmann e Giddens

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    Marília Luz David

    2011-11-01

    Full Text Available http://dx.doi.org/10.5007/1806-5023.2011v8n1p30 O presente artigo discute as contribuições de Anthony Giddens e Niklas Luhmann para uma melhor definição do conceito de risco, tendo em vista o rigor teórico de ambos os autores. Inicialmente é feita uma breve exposição sobre como o conceito de risco passou a ser utilizado na Modernidade e recebeu a atenção de autores das Ciências Sociais, principalmente a partir da abordagem cultura proposta por Mary Douglas. Em seguida, as definições e correspondentes argumentos sobre o conceito de risco em Luhmann e Giddens são apresentados respectivamente. Finalmente, alguns pontos de distanciamento entre os autores são discutidos e de que maneira cada um contribuiu para o debate sobre os riscos na teoria social e para o diagnóstico da Modernidade.

  18. AUTOPERCEPCIÓN DE LOS ADULTOS MAYORES SOBRE SU VEJEZ*

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    Sagrario Garay Villegas

    2010-03-01

    Full Text Available El objetivo de este artículo es aproximarse a los significados y percepciones que tienen los adultos mayores sobre esta etapa de su vida. Para ello utilizaremos herramientas de carácter cualitativo y cuantitativo. Nuestra pretensión de usar ambos métodos es contar con la visión de la población con 60 años o más, sobre aspectos relacionados con su salud física y mental, pero también indagar el los factores que podrían estar influyendo en dicha percepción. Los resultados encontrados nos revelan que existe una asociación muy cercana entre la percepción sobre la salud física y el estado mental de las personas adultas mayores. Palabras clave: vejez; significados; salud mental; salud física y felicidad. * Agradecemos los valiosos comentarios a versiones preeliminares de este documento realizados por la Dra. Verónica Montes de Oca.

  19. Efectos del D-003 sobre patrones conductuales de roedores

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    Lilia Fernández Dorta

    2005-01-01

    Full Text Available El D-003 es una mezcla de ácidos alifáticos de muy alto peso molecular purificada de la cera de la caña de azúcar con efectos hipocolesterolemizante y antiagregante plaquetario demostrados experimentalmente y en estudios clínicos Fase I y II. Los estudios experimentales y clínicos culminados han mostrado que el D-003 es seguro y bien tolerado. Sin embargo, la farmacología de la seguridad de nuevos medicamentos investiga sus efectos sobre diversos sistemas que pudieran ser blancos potenciales de toxicidad. Teniendo en cuenta que los efectos adversos relacionados con el Sistema Nervioso (SN están entre los más frecuentes para diversas áreas terapéuticas, estos estudios suelen investigar los efectos de las sustancias sobre la función y estructura del SN. La investigación de los efectos sobre patrones conductuales de roedores comúnmente representa la primera etapa de estos estudios. De acuerdo con ello, el presente estudio tuvo como objetivo investigar los efectos del tratamiento oral con D-003 (dosis únicas y repetidas sobre la actividad exploratoria, la respuesta al plato caliente, la sujeción y ejecución en varilla rotatoria y la evitación pasiva de una sola prueba de aprendizaje en ratones. Los animales se distribuyeron aleatoriamente en cinco grupos experimentales (10 animales/grupo: uno control que recibió el vehículo goma acacia-H20 y cuatro tratados con D-003 (5, 25, 100 y 400 mg/kg, respectivamente. El tratamiento oral con dosis únicas y repetidas de D-003 (400 mg/kg aumentó de forma modesta, pero significativa, la actividad exploratoria con respecto al grupo control. Sin embargo, el D-003 no modificó la respuesta en el ensayo de plato caliente, la capacidad de sujeción y ejecución motora en varilla rotatoria ni la retención del aprendizaje en el ensayo de evitación pasiva. Se necesitan estudios ulteriores que diluciden las causas por las cuales el D-003 aumenta la actividad exploratoria en ratones y que

  20. Efecto del D-004 sobre patrones conductuales en roedores

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    Daisy Carbajal Quintana

    2007-01-01

    Full Text Available El D-004 es un extracto lipídico de los frutos de la palma real cubana (Roystonea regia que ha mostrado efectos protectores sobre la hyperplasia prostática (HP inducida en roedores con testosterona, pero no con dihidrotestosterona y que además, antagoniza respuestas mediadas por los ¿1-adrenoreceptores prostáticos. El D-004 mostró una modesta acción antiinflamatoria en el modelo de granuloma por algodón, modelo que se acerca más a la condición de inflamación crónica aparejada a la hiperplasia prostatica benigna (HPB en el hombre, lo que podría contribuir, en alguna medida, a su efecto en los modelos de HP. Teniendo en cuenta que los efectos adversos relacionados con el sistema nervioso (SN están entre los más frecuentes para diversas áreas terapéuticas, es obligado investigar los efectos de las sustancias sobre la función y estructura del SN. La investigación de los efectos sobre patrones conductuales de roedores comúnmente representa la primera etapa de estos estudios. El presente estudio investigó los efectos del tratamiento oral con D-004 (dosis únicas y repetidas sobre la actividad exploratoria, la respuesta al plato caliente y a las contracciones inducidas con ácido acético (200, 400 y 800 mg/kg, así como sobre la sujeción en varilla rotatoria y la fuerza de agarre en ratones (250, 500 y 1 000 mg/kg. El tratamiento oral con D-004 no modificó la actividad exploratoria, la respuesta al plato caliente y a las contracciones inducidas con ácido acético, así como la sujeción en varilla y la fuerza de agarre . El tratamiento oral con D-004 en el intervalo de dosis indicado no indujo efectos neurotóxicos, ya que no modificó ninguna de las conductas estudiadas.

  1. Examen de las páginas Web sobre aprendizaje de idiomas. Discursos sobre lengua, aprendizaje y aprendices

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    José Aldemar Álvarez Valencia

    2016-12-01

    Full Text Available Las páginas web de aprendizaje de idiomas se han convertido en una fuente potencial de aprendizaje de idiomas desde la aparición de la World Wide Web. Al igual que cualquier material pedagógico, estas páginas web promulgan visiones sobre la lengua, el aprendizaje y los estudiantes a través de sus diseños semióticos (contenido y diseño estructural. Este estudio es un intento por explorar estas tres dimensiones mediante el examen de la estructura y los contenidos de Pumarosa, una página web de aprendizaje de inglés, y se centra en los usos de una estudiante-usuaria de este entorno virtual. Este estudio de caso se fundamenta en los elementos de la semiótica multimodal y otras estrategias cualitativas de recolección de datos, tales como entrevistas, grabación de la pantalla de acciones en el entorno virtual, y recuerdo estimulado. Los resultados indican que las visiones sobre la lengua, el aprendizaje y la forma en que los usuarios son posicionados en la página web ejercen una gran influencia sobre los significados que la estudiante-usuaria construye alrededor de esos conceptos

  2. transportados por Musca domestica. Lima-Perú.

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    Christian Castillo Elera

    2008-01-01

    Full Text Available Objetivo: Determinar el porcentaje de parásitos de importancia en salud pública que son transportados por Musca domestica. Diseño: Estudio transversal, descriptivo no experimental. Lugar: Instituto de Medicina Tropical ¿Daniel A. Carrión¿, Universidad Nacional Mayor de San Marcos. Participantes: 1867 ejemplares de Musca domestica. Intervenciones: Técnicas de lavado y machacado de moscas; los sedimentos se analizaron con las coloraciones de Lugol y Kinyoun empleando microscopio óptico. Principales medidas de resultado: Análisis en frecuencias absolutas y relativas de parasitos encontrados en las técnicas de machacado y lavado. Resultados: Se hallaron los siguientes parásitos: Endolimax nana (41.13%, Blastocystis hominis (34.68%, Entamoeba hartmanni (10.86%, Entamoeba coli (4.34%, Cyclospora cayetanensis (4.34%, Giardia lamblia (2.17% y Toxocara canis (2,17%. Conclusiones: Los parásitos encontrados en Musca domestica del mercado Caquetá fueron los siguientes: Endolimax nana, Blastocystis hominis, Entamoeba hartmanni, Entamoeba coli, Cyclospora cayetanensis, Giardia lamblia y Toxocara canis, siendo el de mayor frecuencia E. nana en el total de la muestra y a su vez, en el subgrupo de lavado. Se describe el hallazgo de Toxocara canis no reportado en estudios similares.

  3. PARÁSITOS DE IMPORTANCIA EN SALUD PÚBLICA TRANSPORTADOS POR Musca Doméstica. LIMA-PERÚ.

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    Christian Castillo Elera

    2012-02-01

    Full Text Available Objetivo: Determinar el porcentaje de parásitos de importancia en salud pública que son transportados por Musca domestica. Diseño: Estudio transversal, descriptivo no experimental. Lugar: Instituto de Medicina Tropical “Daniel A. Carrión”, Universidad Nacional Mayor de San Marcos. Participantes: 1867 ejemplares de Musca domestica. Intervenciones: Técnicas de lavado y machacado de moscas; los sedimentos se analizaron con las coloraciones de Lugol y Kinyoun empleando microscopio óptico. Principales medidas de resultado: Análisis en frecuencias absolutas y relativas de parasitos encontrados en las técnicas de machacado y lavado. Resultados: Se hallaron los siguientes parásitos: Endolimax nana (41.13%, Blastocystis hominis (34.68%, Entamoeba hartmanni (10.86%, Entamoeba coli (4.34%, Cyclospora cayetanensis (4.34%, Giardia lamblia (2.17% y Toxocara canis (2,17%. Conclusiones: Los parásitos encontrados en Musca domestica del mercado Caquetá fueron los siguientes: Endolimax nana, Blastocystis hominis, Entamoeba hartmanni, Entamoeba coli, Cyclospora cayetanensis, Giardia lamblia y Toxocara canis, siendo el de mayor frecuencia E. nana en el total de la muestra y a su vez, en el subgrupo de lavado. Se describe el hallazgo de Toxocara canis no reportado en estudios similares.

  4. A Comparative Analysis of Intestinal Parasitic Infections between HIV+/AIDS Patients and Non-HIV Infected Individuals

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    EB Kia

    2007-04-01

    Full Text Available Background: The aim of this study was to verify the occurrence of intestinal parasitic infections in human immunodeficiency virus (HIV/acquired immunodeficiency syndrome (AIDS patients in Iran in comparison with non-HIV individuals. Methods: A total of HIV+/AIDS patients (Group I and 1220 clinically healthy individuals (Group II were submitted to coproparasitological examination from 2003 to 2005. Results: The overall prevalence of intestinal parasites in group I and group II was 11.4% and 11.6%, respectively, without significant difference between two groups. The prevalence of infection for each helminth and pathogenic protozoan, in every group, was as follows: Group I: Blastocystis hominis (6.1%; Giardia lamblia (4.2%; Cryptosporidium spp. (0.9%; Isospora belli (0.26%; Strongyloides stercoralis (0.26%; Hymenolepis nana (0.13%; and Rhabditis axei (0.13%. Group II: Blastocystis hominis (6.5%; Giardia lamblia (4.1%; Strongyloides stercoralis (0.33%; Hymenolepis nana (0.16%; and Trichostrongylus sp. (0.16%. Although the prevalence of infection for extracellular parasites was not statistically different between two groups, however, the infection rates for enteric coccidians including Cryptosporidium spp. and I. belli were significantly higher in patients at AIDS stage than Group II. Conclusion: The results emphasize the needs for especial consideration of enteropathogenic intracellular coccidians in immunocompromised patients.

  5. Prevalence of Strongyloides stercoralis and other intestinal parasitic infections among mentally retarded residents in central institution of southern Iran

    Institute of Scientific and Technical Information of China (English)

    Azar Shokri; Khojasteh Sharifi Sarasiabi; Saeed Hosseini Teshnizi; Hamid Mahmoodi

    2012-01-01

    Objective: To determine the prevalence of intestinal parasitic infections among mentally retarded residents of rehabilitation center of Bandar Abbas, Hormozgan province, southern Iran. Methods: A cross-sectional study was carried out in central rehabilitation institute of Hormozgan province in summer 2010. Fecal samples of all 133 residents (72 males, 61 females) aged 3-52, were collected in triplicate. Specimens were examined by direct smear, formalin-ether concentration techniques and stained by permanent Trichrome, Ziehl-Neelsen stains. Statistical analysis was conducted by SPSS 13.5. Results:Intestinal parasitic infections were seen in 48.5%(64 out of 133 subjects:53.4%in males and 46.6%in females). Strongyloides stercoralis with 17.3%showed the highest incidence followed by Entamoeba coli (9.8%), Blastocystis hominis (7.5%), Giardia lamblia (2.3%), Endolimax nana (2.3%), Hymenolepis nana (0.8%), Oxyuris vermicularis (0.8%), and Chilomasix mesnili (0.8%). Double infections were found to be as: Strongyloides stercoralis+Giardia lamblia (2.3%), Entamoeba coli+Giardia lamblia (1.5%), Entamoeba coli+Blastocystis hominis (1.5%), Oxyuris vermicularis+Entamoeba coli (0.8%), Strongyloides stercoralis+Entamoeba coli (0.8%), respectively. Conclusions:Our findings reveal that strongyloidiasis is a common disease among mentally retarded population in southern Iran.

  6. QUESTÕES SOBRE A PENA DE MORTE

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    SOUSA, L.N.

    2015-12-01

    Full Text Available Pelo menos, desde o Código de Hamurabi as punições para atos desviantes tem sido um foco de reflexão. Este trabalho encerra essa reflexão acerca de aspectos em torno do tema pena de morte. Além de feito político contra o crime, também seria um ato com fins vingativos, fundamentado na violação da dignidade humana, o que justifica a necessidade de haver uma rigorosa análise sobre tal questão, visando os argumentos lógicos, baseados na realidade social brasileira. Alguns estudos demonstram que a percepção da classe menos favorecida é que já existe, de certa forma, uma pena de morte informal no Brasil, praticada pela polícia ou grupos de extermínio. No entanto, diversos setores defendem a pena de morte em nome dos direitos humanos. O debate sobre o referido tema não deve limitar-se às questões relacionadas ao desenvolvimento moral individual, antes deve incluir também a análise de fatores históricos, culturais, econômicos, políticos e ideológicos. Este estudo tem como objetivo coletar dados bibliográficos, bem como dados sobre as opiniões que as pessoas em Campos dos Goytacazes têm sobre a pena de morte. A pesquisa feita é de caráter exploratório. Utilizaram-se as metodologias pertinentes a uma pesquisa bibliográfica. Foi realizada com moradores da cidade de Campos dos Goytacazes/RJ, especificamente com 40 jovens e adultos, de gênero masculino e feminino, com idades entre 18 e 48 anos, sendo a maioria mulheres. A coleta de dados foi feita através de questionários fechados, do tipo likert. Cerca de 48% da amostra concorda com a sentença pena de morte. As respostas quanto a não ter opinião formada sobre o assunto foram minoria, somando 20% ao todo, restando 32% de sujeitos que declararam ser contrários. Metade dos sujeitos não concordou que a sentença seria aplicada corretamente no Brasil. A minoria concordou que a lei teria uma aplicabilidade positiva no país. Aproximadamente 43% dos sujeitos pesquisados

  7. Prevalence of Intestinal Parasites and Clinical Manifestations in Children

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    M Ebadi

    2007-06-01

    Full Text Available Background: Diagnosis and treatment of parasitic infections are very important because of pathologic changes and clinical symptoms produced in the host, and for taking measures against them. These diseases have more adverse effects and more importance in children. This study was aimed at determining the prevalence of intestinal parasites and their clinical manifestations in children 0-14 years old referred to Yazd Central Laboratory. Methods: The present cross- sectional descriptive study was performed during the April 2005 to September 2006, with data obtained from the stool samples of 1500 children, using both wet mount smear (physiologic saline and Lugol΄s solutions and formalin-ethyl acetate concentration method for detection of the intestinal parasites and also with the tape test for eggs of Enterobius vermicularis and Taenia. Results: From 1500 examined stool samples, 128(8.5% cases were positive for one of the intestinal parasites, including 67(52.3% females and 61(47.7% males. Ninety five percent of positive cases were infected with protozoa and 5% with helminths. The most frequent protozoans were Blastocystis hominis 41.3%, Giardia lamblia 33.6%, and Entamoeba coli 14.7%. Enterobius vermicularis (4.3% and Hymenolepis nana (0.7% were the helminth species. The most abundant infection rate was shown in the 5-9 years old group, with a significant difference compared with other age groups (P< 0.05. About 87% of children infested with Blastocystis hominis showed more than 5 Blastocystis per 400x microscope fields, and 100% of children infested with Giardia lamblia and Chilomastix mesnili had clinical symptoms. Conclusion: In this study, lower contamination rates in comparison with the similar studies conducted in other regions of the country was seen, which may be as a result of the hot and dry climate and improved personal hygiene and public health services. There is a need for further studies about the prevalence and clinical symptoms of

  8. EL IMPUESTO SOBRE BEBIDAS AZUCARADAS EN ESPAÑA

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    Vicente Ortún

    2016-01-01

    Full Text Available Este artículo aporta una revisión crítica acerca de los retos a los que se enfrentan los impuestos sobre las bebidas azucaradas como instrumento de políticas de salud, para revertir la tendencia epidémica de la obesidad. Se valoran las experiencias de los países más significados, en particular México, y se reflexiona sobre el contrapeso que ejerce la industria a las políticas antiobesidad y el poder de los lobbies. Esas políticas impositivas en pro de la salud pública han de sobreponerse a la enorme fuerza de la industria, que es ejercida en varios niveles –ciencia e investigación, reputación de marca, influencia en reguladores–. Se sugiere que un impuesto específico sobre bebidas azucaradas tiene bastante potencial para reducir enfermedades no transmisibles y riesgos –diabetes mellitus, hipertrigliceridemia, lipoproteínas de baja densidad, hipetensión diastólica–, a través de la reducción del consumo, al ser alta la elasticidad del precio de estas bebidas. Además, los efectos incluso se amplifican a medio plazo, una vez establecidos nuevos hábitos de consumo más saludable. Los impuestos podrían fomentar la innovación empresarial sin infligir costes de pérdida de empleos y contribuirían a reducir el gradiente social de la obesidad.

  9. Estado del arte del conocimiento sobre perifiton en Colombia

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    Yimmy Montoya Moreno

    2013-12-01

    Full Text Available El perifiton es un componente de las comunidades bióticas acuáticas, su estudio es importante tanto desde la perspectiva ecológica, para comprender el funcionamiento de los ecosistemas acuáticos, como desde el punto de vista ambiental, pues su composición y estructura pueden servir como indicadores de la calidad del agua y de procesos que como la contaminación puedan estar afectando a los ecosistemas. La investigación sobre el perifiton es compleja; la definición misma de perifiton aún genera controversias. Sumado a esto, la variedad de metodologías para su estudio integral, la diversidad de arquitecturas de la matriz perifítica, la gran diversidad de ecosistemas y la ausencia de escuelas taxonómicas en el país, contribuyen a que sean pocos los trabajos que se publican sobre el tema, lo que incrementa el valor e importancia de los estudios reseñados en esta revisión. El objetivo de este artículo es dar a conocer el estado del arte en la investigación sobre el perifiton en Colombia, mediante el análisis de diferentes líneas de investigación que se han desarrollado y su grado de avance. Con este fin, se examinaron 84 trabajos a los que los autores tuvieron acceso. La mayoría de investigaciones consultadas en este trabajo (90%, corresponden al estudio de ficoperifiton, el 10% restante incluyó ambos componentes del perifiton y se encontró una dedicada exclusivamente al zooperifiton. Sólo el 10,5% de las investigaciones (8 trabajos se han dedicado al estudio taxonómico detallado, el resto de publicaciones se orientan al estudio de la estructura y dinámica de la comunidad perifítica.

  10. Dos hipótesis sobre el presidencialismo autoritario

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    Soledad Loaeza

    2013-01-01

    Full Text Available El presidencialismo autoritario mexicano ha sido objeto de muchas reflexiones y opiniones. No obstante, se ha impuesto un paradigma presidencialista que privilegia la perspectiva personalizada e idiosincrática del ejercicio del poder, con un importante componente cultu- ralista. Aquí se propone, por una parte, una perspectiva histórica que contextualiza las accio- nes presidenciales en el período 1944-1970; por la otra, se parte de dos presupuestos generales. Uno, sostiene que los presidentes mexicanos actuaban bajo importantes restricciones y según los ritmos de un proceso que avanzó en episodios, más que en forma lineal o acumulativa; el otro destaca la limitación geopolítica que se deriva de la vecindad con Estados Unidos que incidió sobre las acciones presidenciales, pero también sobre el desarrollo institucional del país. La evolución del presidencialismo autoritario lleva la huella de esta influencia. Este artículo introduce elementos para la discusión de las hipótesis. La primera parte hace una revisión crítica del paradigma presidencialista; la segunda expone las restricciones que se derivaban de la vecindad con Estados Unidos. La tercera y última está dedicada a la descripción del tipo de episodios que Pueden servir para ilustrar la evolución del presidencialismo autoritario. Las dos hipótesis que aquí se presentan han guiado una investigación más amplia sobre el impacto de la Guerra Fría en México y el desarrollo del presidencialismo.

  11. Propuesta de programa educativo sobre enfermedades del cuello uterino

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    Grisel Mariela Mass Basulto

    2011-10-01

    Full Text Available Fundamento: El cáncer ginecológico está entre las más frecuentes causas de muerte en la población femenina. En Cuba, el diagnóstico precoz de tal afección está entre los programas priorizados del Sistema de Salud Pública; pero también es necesario enfatizar en la prevención, que puede lograse, sobre todo, desde la atención primaria de salud, mediante la implementación de programas educativos. Objetivo: Diseñar un programa educativo sobre afecciones del cuello uterino. Métodos: Estudio descriptivo y retrospectivo, en el período comprendido del 1 de enero de 2008 al 1 de enero de 2009. Se analizaron variables como edad, escolaridad, primeras relaciones sexuales, número de partos, número de compañeros sexuales, uso de anticonceptivo, antecedentes de infecciones de transmisión sexual, hábitos tóxicos y nivel de conocimiento. Se aplicó el criterio de expertos para la validación de la propuesta educativa. Resultados: La mayoría de las mujeres tuvieron relaciones sexuales precoces, cuatro o más compañeros sexuales y eran fumadoras, mientras que el total de ellas había presentado alguna vez infecciones vaginales. El nivel de conocimiento que predominó fue regular. Conclusiones: Es evidente que en la población estudiada se concentran varios factores de riesgo para el cáncer de cuello uterino. El Programa Educativo sobre enfermedades del cuello uterino, fue validado mediante criterio de expertos, los cuales coincidieron en que tiene gran actualidad científica, congruencia interna y en que favorece el proceso de preparación de la mujer para evitar ese tipo de enfermedades.

  12. Sobre os sistemas de referência celeste

    Science.gov (United States)

    Poppe, P. C. R.; Martin, V. A. F.

    2003-02-01

    Apresentamos neste trabalho, algumas discussões sobre os sistemas de referência utilizados em Astronomia. Claramente, não é possível esgotar todo este assunto num único texto, mas esperamos, contudo, que o presente material possa ser apreciado nos cursos de Introdução à Astronomia, que estão cada vez mais presentes nas atuais propostas curriculares das graduações de Física. As discussões pertinentes às "Bases de Referência Celeste", serão apresentadas em um outro trabalho.

  13. Anotaciones sobre la escritura de lo político

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    Markus Schultze-Kraft

    2009-06-01

    Full Text Available Escribir bien sobre lo político es una forma de arte. Hoy, pocos escritores entienden el significado de esta premisa. Constantemente nos enfrentamos a artículos, ensayos, informes y libros mediocres y sesgados que pretenden ser “políticos” y “científicos”, pero que en realidad son expresiones de sensacionalismo y confusión ideológica o de falta de conocimiento empírico del autor, y generalmente de perspectiva.

  14. SOBRE A CATEGORIA DA MEDIAÇÃO EM ADORNO

    OpenAIRE

    Correio, Antônio Marcos V. Sanseverino

    2008-01-01

    O PRESENTE ESTUDO TOMA COMO CENTRO O MODO COMO THEODOR ADORNO DEFINE A MEDIAÇÃO ENTRE ARTE E SOCIEDADE. O PASSO SEGUINTE É VERIFICAR O MODO COMO ESSA CATEGORIA APARECE EM DIVERSOS ENSAIOS DO AUTOR: ENSAIO COMO FORMA; PALESTRA SOBRE LÍRICA E SOCIEDADE; POSIÇÃO DO NARRADOR NO ROMANCE CONTEMPORÂNEO ENTRE OUTROS. O INTERESSE É EVIDENCIAR O MOVIMENTO DE LEITURA DE ADORNO, QUE, AO ESTABECERA PRIMAZIA DO OBJETO, DESENTRANHA A MEDIAÇÃO DO PRÓPRIO OBJETO E IMPEDE A RECEPÇÃO IMEDIATA DA OBRA DE ARTE....

  15. Consideraciones sobre los sistemas electorales a principios del siglo XXI

    OpenAIRE

    Humberto Nogueira Alcalá

    2007-01-01

    El artículo realiza un análisis sobre los diversos componentes de un sistema electoral, poniendo énfasis en la conformación de los distritos electorales, los tipos de candidaturas, el método de escrutinio y la barra o umbral de representación, considerando que si bien los sistemas electorales influyen en el sistema de partidos políticos, ellos no son determinantes en su conformación, producto de la existencia de otros factores culturales, históricos y realidades socioeconómicas...

  16. NOTAS SOBRE A ‘VIDA DE PIRRO’

    Directory of Open Access Journals (Sweden)

    Rodrigo Pinto de Brito

    2014-08-01

    Full Text Available Uma investigação da atribuição de um determinado arcabouço conceitual (como epoché, ataraxia e adiaforia à Pirro, de acordo com o relato de sua “Vida”, por Diógenes Laércio, em D.L. IX, 61-71. Assim, oferecendo uma interpretação sobre a possível utilização destes conceitos pelo próprio Pirro.

  17. Resúmenes de los trabajos sobre trastornos del movimiento

    Directory of Open Access Journals (Sweden)

    Congreso Nacional de Neurología

    2010-03-01

    Full Text Available En el salón sobre trastornos del movimiento se presentaron un total de 10 trabajos científicos en los cuales se destacó tanto la participación de autores nacionales como extranjeros. A continuación se pueden var en este apartado  los resúmenes de los artículos en el mismo orden que fueron presentados. También se puede encontrar en ellos las direcciones electrónicas de los responsables.

  18. Diálogo sobre plantas medicinais: significados de escolares

    OpenAIRE

    Ceolin, Silvana; Mendieta, Marjoriê da Costa; Ceolin, Teila; Lopes, Ana Carolina Padua; Heck, Rita Maria

    2016-01-01

    O estudo teve como objetivo conhecer os significados atribuídos pelos escolares ao diálogo sobre plantas medicinais no cuidado à saúde. Pesquisa qualitativa realizada com 65 escolares de duas escolas do município de Pelotas/RS, no período de setembro de 2011 a agosto de 2012. O material analisado foi produzido ao final de 18 oficinas a partir de redações escritas por escolares e de grupos focais. Os encontros possibilitaram estimular nos educandos valores direcionados ao reconhecimento do sab...

  19. Questões sobre A medida, de Brecht

    Directory of Open Access Journals (Sweden)

    Lucianno Gatti

    2012-09-01

    Full Text Available O texto discute a peça "A medida", de Bertolt Brecht, a partir de uma análise da forma da peça de aprendizagem, bem como de sua polêmica recepção. Apresenta-se o conceito de “refuncionalização” como estratégia político-artística inerente à tal forma de espetáculo, para então debater a atualidade da peça, bem como a relevância dos textos de Walter Benjamin para os estudos sobre Brecht.

  20. Reflexiones sobre la revisión de traducciones

    OpenAIRE

    2009-01-01

    El objetivo de nuestro estudio es el de reflexionar sobre un tema que durante mucho tiempo ha sido descuidado por los traductólogos a pesar de su crucial importancia en todo el proceso de traducción, a saber, la revisión. En este artículo, en primer lugar, abordaremos el problema de una definición clara de este término, en segundo lugar, las diferentes clases de revisión según el sujeto o sujetos que la realizan y finalmente, comentaremos el problema de la objetividad en la revisión y present...

  1. Ensayos empíricos sobre desigualdades regionales

    OpenAIRE

    Duro Moreno, Juan Antonio

    2003-01-01

    Esta Tesis Doctoral versa sobre desigualdades regionales. Integra una serie de trabajos que se detienen en medir y explorar las desigualdades regionales, típicamente en España, a partir de distintos enfoques analíticos. En los primeros dos capítulos, se hace hincapié en la medición y descomposición de la desigualdad regional a través de índices sintéticos y, en particular, se subrayan las atractivas propiedades que brinda el índice de Theil población-ponderado, o segunda medida de Theil. En e...

  2. Ensayos empíricos sobre desigualdades regionales tesis doctoral /

    OpenAIRE

    Duro Moreno, Juan Antonio

    2003-01-01

    Consultable des del TDX Títol obtingut de la portada digitalitzada Esta Tesis Doctoral versa sobre desigualdades regionales. Integra una serie de trabajos que se detienen en medir y explorar las desigualdades regionales, típicamente en España, a partir de distintos enfoques analíticos. En los primeros dos capítulos, se hace hincapié en la medición y descomposición de la desigualdad regional a través de índices sintéticos y, en particular, se subrayan las atractivas propiedades que brind...

  3. Breves apuntes sobre el cambio climático

    OpenAIRE

    Zambrano, E.

    2007-01-01

    Las estadísticas que se manejan en muchos centros de investigación climática, los últimos 10 años han sido los más cálidos desde que se llevan registros, los científicos anuncian que en el futuro el ambiente será aún más tórrido. La mayoría de los expertos están de acuerdo en el sentido de que los humanos ejercen un impacto directo sobre este proceso de calentamiento, proceso generalmente conocido como el "efecto invernadero".

  4. Verifique sus conocimientos sobre ecografía

    OpenAIRE

    Galimany Masclans, Jordi; Pernas Canadell, J. C.

    2011-01-01

    La presente entrega de la serie de Nursing sobre las pruebas complementarias está dedicada a la ecografía. La ecografía está ampliamente difundida como exploración de diagnóstico por la imagen, se utiliza en multitud de procedimientos para diagnóstico inicial y también como apoyo en localizaciones prequirúrgicas, punciones y drenajes. La ecografía es una técnica sencilla, mínimamente invasiva y con gran capacidad diagnóstica. Estas características, junto con su coste reducido en comparación c...

  5. Creencias de los Hombres sobre lo que Significa ser Padre

    OpenAIRE

    Consuelo Paterna; Carmen Martínez; Joaquín Rodes

    2005-01-01

    Durante estos últimos años la definición del rol paternal ha sufrido un cambio respecto de lo que tradicionalmentese asumía como creencias sobre dicho rol. En este sentido, el objetivo de este estudio es analizar la representaciónsocial de las condiciones de paternidad y no paternidad en una muestra de 80 padres con empleo. Se realizó unanálisis de contenido de las respuestas suscitadas a través de una tarea de libre asociación. Los resultados muestranque los hombres describen la condición de...

  6. TRES NOTAS SOBRE EL SUJETO ANTICAPITALISTA POLIMÓRFICO

    OpenAIRE

    Tischler, Sergio; Instituto de Ciencias Sociales y Humanidades “Alfonso Vélez Pliego” de la Benemérita Universidad Autónoma de Puebla

    2013-01-01

    Con el objetivo de pensar el cambio revolucionario, se exponen ciertas nociones orientadas a dar cuenta de la crisis del sujeto clásico de larevolución y de la emergencia de una nueva forma de pensar la lucha de clases. En este sentido, el autor parte de algunos conceptos desarrollados por Benjamin en su tesis sobre el concepto de la historia, particularmente aquellos que permiten ver el concepto de lucha de clases en clave antiprogresista y pensar al sujeto revolucionario no como parte del c...

  7. Duas ou três coisas sobre cinema

    OpenAIRE

    Rodrigues, Alda Alexandra Pastor Barreto Sarmento

    2008-01-01

    Tese de mestrado em Teoria da Literatura apresentada à Faculdade de Letras da Universidade de Lisboa, 2009 Esta tese parte da análise dos filmes The Birds, de Alfred Hitchcock, e Journal d'un curé de campagne, de Robert Bresson, e dos depoimentos destes dois realizadores, para fazer uma descrição geral do cinema e da importância do espectador na construção do significado de um filme. Nela são tratadas questões linguísticas, literárias e epistemológicas, sobre usos de palavras em filmes, so...

  8. Notas sobre tres especies de Gigartinaceae (Rhodophyta del litoral peruano

    Directory of Open Access Journals (Sweden)

    Martha Calderón

    2011-05-01

    Full Text Available La gran variabilidad morfológica de la familia Gigartinaceae ha producido constantes cambios taxonómicos en sus especies miembros. Tradicionalmente su taxonomía ha estado basada en caracteres de la morfología externa; sin embargo, recientes estudios morfológicos, respaldados con trabajos moleculares, han delimitado los géneros en base a al desarrollo del cistocarpo y de los tetrasporangios. El presente trabajo revisa y comenta sobre las estructuras morfológicas vegetativas y reproductivas de tres especies presentes en Perú: Chondracanthus chamissoi, Mazzaella denticulata y Chondrus canaliculatus.

  9. La red sobre trabajo infantil peligroso (Red Tip)

    OpenAIRE

    Walter Varillas

    2003-01-01

    En el mundo, aproximadamente 351.7 millones de niños entre 5 y 17 años realizaban algún tipo de actividad económica, de ellos 170.5 millones (48.5%) realizaban algún tipo de trabajo considerado peligroso. Un alto porcentaje se encuentra en la agricultura, otros en minas, manufacturas, ladrilleras, predominantemente en la economía informal. El Convenio 138 (cobre la edad mínima de admisión en el empleo) de la OIT y el Convenio 182 (sobre las peores formas de trabajo infantil), definen como tra...

  10. Opinião do pai sobre o aleitamento materno

    OpenAIRE

    ROSINEIDE SANTANA DE BRITO; ETENIGER MARCELA FERNANDES DE OLIVElRA

    2006-01-01

    Estudio cualitativo desarrollado en Natal/RN con 13 hombres cuyos hijos estaban en período de lactancia natural. Tuvo por objetivo verificar la opinión del padre sobre la lactancia materna. Los datos se obtuvieron a partir de entrevista parcialmente estructurada y tratados siguiendo la técnica de análisis temático propuesta por Bardin. Los padres atribuyeron valores importantes a la lactancia relacionándolos al hijoya la compañera. Con relación al niño, verificamos conocimiento acerca de los ...

  11. Vocabulario sobre el cambio climático

    OpenAIRE

    2009-01-01

    El trabajo está basado en la colaboración con el banco de datos terminológicos y lingüísticos del Gobierno de Canadá (Termium). Este trabajo terminológico ha nacido de la necesidad de ampliar un vocabulario sobre el cambio climático con equivalentes en inglés y en francés, a un vocabulario con equivalentes en inglés, francés, español y catalán.

  12. Ferropenia: impacto sobre el estado de salud mental

    OpenAIRE

    Sánchez Jiménez, Apolonia

    2016-01-01

    La deficiencia de hierro y la anemia por déficit del mismo, son trastornos nutricionales y hematológicos frecuentes en el mundo y afectan a una población estimada de 2.000.000 de personas. La deficiencia de hierro tiene efectos adversos sobre la actividad de numerosas enzimas y en bebés pueden provocar alteraciones del crecimiento y del desarrollo intelectual. Los aspectos hematológicos de la deficiencia de hierro no son específicos, y con demasiada frecuencia se confunden con otras causas d...

  13. Claustre Obert: Carmen Aranegui parla sobre arqueologia i identitat

    OpenAIRE

    Aranegui Gascó, Carmen

    2013-01-01

    La catedràtica d'Arqueologia de la Universitat de València Carmen Aranegui planteja un debat sobre la construcció de les identitats a través de les cultures ibèriques. Carmen Aranegui participarà hui dijous, 18 d'abril, en els debats de Claustre Obert amb la conferència 'Allò cultural i allò nacional. Els ibers, arqueologia i identitat'. La xarrada tindrà lloc a l'Aula Magna del Centre Cultural La Nau, a les 18:30 hores i es retransmet en directe per MediaUni.

  14. Aspectos ideológicos de los discursos sobre drogas.

    OpenAIRE

    Campos Aparicio, Carmen

    2014-01-01

    Existen otras maneras de explicar los consumos de drogas, diferentes a la ideología hegemónica representada por -y en- los discursos oficiales sobre drogas. Revisaremos algunos aspectos ideológicos en torno a las drogas así como otros postulados teóricos menos convencionales acerca del uso de drogas: Por una parte, encontramos autores que narran la historia de los consumos de drogas (ilícitas) como una cuestión de clase social. Por otro lado, encontramos el enfoque marxista, pa...

  15. La consideración contable del Impuesto sobre Sociedades

    OpenAIRE

    Lallana Sotillos, Carlos

    1996-01-01

    Las teorías sobre la Hacienda Pública censuran la existencia de un impuesto , como el de sociedades, destinado a gravar la renta de unos sujetos que solamente actúan como seres racionales en el orbe jurídico. Por tanto, y según las referidas teorías, dicho tributo no lo van a soportar las sociedades mercantiles, sino los titulares de su capital. En consecuencia, son las personas físicas , dueñas del patrimonio empresarial, las que resultan doblemente penalizadas: por medio de sus impuestos pa...

  16. Notas Sobre o Significado Político do Futebol

    Directory of Open Access Journals (Sweden)

    Nildo Viana

    2010-08-01

    Full Text Available

    O presente artigo traz uma breve discussão na forma de notas sobre o significado político do futebol. Neste contexto, mostra o significado conservador do futebol através de vários aspectos: construção de identidade nacional, axiologia, representações ilusórias, evasão e a sua possibilidade de carregar uma potencialidade emancipadora.

  17. Stuart Hall sobre “hacer estudios culturales”

    OpenAIRE

    Mato, Daniel; Universidad Nacional Tres de Febrero (Untref), Buenos Aires, Argentina

    2016-01-01

    Traducción de Emeshe Juhász MininbergEmeshe Juhász Mininberg es escritora e investigadora independiente. Doctora en Filosofía y Letras, Yale University, Estados Unidos. Traductora y editora de textos de crítica cultural y de artes plásticas. Ha publicado varios artículos sobre ciudadanía, cultura e identidad nacional en tiempos de globalización, que aparecen, entre otros, en Cultura, política y sociedad: perspectivas latinoamericanas (Clacso, 2005) y en el Diccionario de estudios culturales l...

  18. Ejercicio de autoevaluación sobre alcalinidad en agua.

    OpenAIRE

    Milla González, Miguel

    2011-01-01

    El ejercicio de autoevaluación contiene 12 cuestiones de entrada múltiple que pueden responderse por páginas (en este caso la corrección es página a página) o de forma global (la calificación se obtiene al finalizar el test). Se pueden hacer correcciones en las dos modalidades antes de obtener la calificación del ejercicio. Las cuestiones tratan sobre la determinación ácido-base de la alcalinidad del agua.

  19. Efectos del vendaje neuromuscular sobre la flexibilidad del raquis lumbar

    OpenAIRE

    A.M. Labrador-Cerrato; P. Ortega Sánchez-Diezma; G. Lanzas Melendo; Gutiérrez-Ortega, C.

    2015-01-01

    Introducción: El vendaje neuromuscular es una técnica que produce una estimulación muy selectiva sobre la piel a través de la aplicación de unas vendas elásticas especiales con el fin de lograr cambios propioceptivos, aumento o inhibición del tono muscular y mitigación de algias, entre otros. Objetivos: Comprobar si la aplicación del vendaje neuromuscular permite aumentar la flexión del raquis lumbar comparándola con otras técnicas de vendaje placebo (esparadrapo rígido convencional; Omniplas...

  20. Sobre a bondade a partir da literatura do holocausto

    Directory of Open Access Journals (Sweden)

    Márcio Bruno Barra Valente

    2015-11-01

    Full Text Available Em termos filosóficos, a bondade é uma efetivação da possibilidade de homens e mulheres se colocarem em relações dialógicas autênticas, isto é, constituídas pela abertura e responsabilidade ética pelo Outro mesmo em situações de exceção. Este artigo objetiva construir uma proposta de investigação sobre a bondade a partir da literatura do holocausto.

  1. Sobre o calculo diferencial paraconsistente de da Costa

    OpenAIRE

    2004-01-01

    Resumo: O objetivo deste trabalho é desenvolver alguns principios básicos de um Cálculo Diferencw Paraconsistente. Como elementos motivadores para isso, apresentamos, na Introdução, alguns dados sobre o desenvolVuI1ento do cálculo diferencial, desde suas origens ao advento da anãlise não-standard, bem como relativos à história da lógica e à da teoria de conjuntos, focalizando os sistemas lógicos paraconsistentes e as teorias paraconsistentes de conjuntos. Apresentamos, no Capítulo 1, propried...

  2. Actividad antimicrobiana del OLEOZON® sobre Staphylococcus aureus y Pseudomonas aeruginosa

    OpenAIRE

    V. Curtiellas; M Gómez; O. Ledea; Fernández, I.; Sánchez, E.

    2005-01-01

    La actividad antimicrobiana de los aceites vegetales ozonizados suele atribuirse a la acción de los compuestos peroxídicos presentes en los mismos sobre las biomoléculas más sensibles al ataque oxidante, como son los lípidos insaturados y las proteínas que presentan grupos sulfidrilos (SH). Con el objetivo de caracterizar la actividad in vitro del aceite de girasol ozonizado, OLEOZON®, se realizó un estudio empleando las cepas S. aureus ATCC 25923 y P. aeruginosa ATCC 27853. Se determinaron l...

  3. Nota sobre el archivo de la familia Pardo Gil Taboada

    Directory of Open Access Journals (Sweden)

    Saurín de la Iglesia, María Rosa

    2007-12-01

    Full Text Available The intention of the bequest of the archives of Pardo Gil-Taboada to the Archive of the Kingdom of Galicia was that of establishing the genealogy of those who were the original source of such document. These notes attempt to sketch the profile of a noble family from they left the first traces of their existence, to well into the XX century. Going beyond the simple enumeration of individuals – “as if the nobility had no other function than the reproductive” – requires the addition of evidence about the way individuals behaved and collective traits and predispositions of the family group. Hence it is hoped that in future researchs this guide will facilitate and provide data about the formation of a family heritage and criteria on its administration, on the role played by women in the accumulation of wealth, attitudes towards life and the participacion by the nobility in the economic development of Galicia, and other aspects which may be of interest to both historians of sociology and economics.

    La donación del archivo Pardo Gil-Taboada al del Reino de Galicia requería establecer la genealogía de quienes originaron tales documentos. Estos apuntes intentan trazar el perfil de una familia hidalga desde que empezó a dejar señales de su existencia hasta bien entrado el siglo XX. Superar la simple enumeración de personas - “como si la nobleza no hubiera tenido más función que la reproductora”, en palabras de Domínguez Ortiz – suponía añadir indicaciones sobre comportamientos individuales y tendencias colectivas del grupo familiar y eso es lo que se ofrece aquí sinteticamente, como una guía de urgencia que anuncie a futuros investigadores lo que podrán hallar en esta documentación: datos sobre la formación de un patrimonio familiar y sobre los criterios de administrarlo, sobre el papel de las mujeres en la acumulación de bienes, actitudes ante la vida, participación de la hidalguía en el crecimiento económico de

  4. Seis Tesis no convencionales sobre participación

    Directory of Open Access Journals (Sweden)

    Bernardo Kliksberg

    1999-08-01

    Full Text Available Si bien hasta hace pocos años la participación generaba mucha polémica, actualmente se esta transformando en un consenso y gran parte de los organismos internacionales la están adoptando como estrategia de acción. Este trabajo plantea una serie de tesis sobre aspectos claves del tema. El objetivo no es dar un tratamiento exhaustivo a ninguno de los temas planteados, sino estimular el análisis colectivo de la misma.

  5. Desenvolvimento da Tanatologia: estudos sobre a morte e o morrer

    OpenAIRE

    Maria Julia Kovács

    2008-01-01

    Este artigo discute os principais temas e pesquisas na área da Tanatologia, estudos sobre a morte e o morrer. São apresentados os autores pioneiros que escreveram as primeiras obras de sistematização da Tanatologia entre os quais: Herman Feifel, Robert Kastenbaum e Elizabeth Kübler-Ross, e os principais temas de estudo: luto, violência e guerra, a morte e a TV, cuidados a pacientes gravemente enfermos, além da formação de profissionais da área de saúde e educação para lidar com pessoas vivend...

  6. Principios y directrices sindicales sobre la eficacia del desarrollo

    OpenAIRE

    2011-01-01

    El presente documento de política presenta la visión y los valores de las organizaciones sindicales sobre la Eficacia del Desarrollo. Éstos encuentran su expresión en la articulación de los Principios y Directrices, elaborados en base a consultas de las organizaciones sindicales representativas tanto del Norte como del Sur. La onfederación Sindical Internacional (CSI) coordinó este proceso, con el apoyo de sus estructuras regionales, organizaciones afiliadas, organizaciones solidarias y feder...

  7. Aprender sobre inmigración en tiempos de incertidumbre

    OpenAIRE

    Essomba, Miquel Àngel

    2012-01-01

    La mirada sobre la inmigración en tiempos de crisis y de cambio de paradigma exige una actualización de las propuestas didácticas y de los recursos educativos. Dos son los enfoques de las estrategias que pueden cambiar conceptos y actitudes: el cognitivo y el socioafectivo. El primero propone aportar conocimientos para combatir la ignorancia que desata los prejuicios; el segundo invita a los participantes en los procesos de aprendizaje a ponerse en la piel del otro para generar empatía.

  8. Molecular epidemiological study of Ureaplasma urealyticum and Mycoplasma hominis in urogenital tract of elementary and high school students in Qingdao area%青岛地区中小学生泌尿生殖道解脲脲原体及人型支原体分子流行病学调查

    Institute of Scientific and Technical Information of China (English)

    孟慧娟; 史同新; 孙文龙; 张骋; 李民

    2012-01-01

    目的 探讨解脲脲原体(Uu)和人型支原体(Mh)在健康中小学生泌尿生殖道中的定植情况,以及Uu的分群分型状况.方法 培养法对青岛地区957名中小学生的尿沉渣进行Uu和Mh培养,培养阳性者用PCR确证,并对Uu阳性标本进一步分群分型.结果 Uu阳性46例,占4.81%,生物一群占69.57%,生物二群30.43%.小学生Uu阳性12例,占2.97%,生物一群9例(75.00%),生物二群3例(25.00%).初中生Uu阳性8例,占3.08%:生物一群5例(62.50%),生物二群3例(37.50%).高中生Uu阳性26例,占8.87%:生物一群18例(69.23%),生物二群8例(30.77%).Mh阳性9例(0.94%),其中小学生Mh阳性1例,初中生Mh阳性1例,高中生Mh阳性7例.3个组Uu阳性率女生均高于男生(P<0.05).结论 Uu和Mh在中小学生泌尿生殖道中存在无症状的携带状态.尽管各年龄组检出的Uu均以生物一群为主,但生物二群也占有不小的比例,提示Uu可能是健康中小学生人群正常定植菌,且女性携带率高于男性.%Objective To detect Ureaplasma urealyticum (Uu) and Mycoplasma hominis (Mh) in urogenital tract of healthy elementary and high school students,and to determine the biovars and serotypes of Uu isolates.Methods Urine specimens were collected from 957 healthy school children,including 404 elementary school students,260 junior high school students and 293 senior high school students,and submitted for Uu and Mh culture in selective liquid medium.Thc specimens positive for Uu and (or) Mh culture were subsequently subjected to PCR.The biovars and serotypes of Uu were determined.Results Uu was detected in 4.81% (46/957) of the school children.Among the 46 Uu positive specimens,32 (69.57%) harbored Ureaplasma Parvum (biovar 1 ),14 (30.43%) Ureaplasma urealytieum (biovar 2); 12 (2.97%) were from elementary school students,including 9 (75.00%) cases of Ureaplasma Parvum and 3 (25.00%) Ureaplasma urealytieum,8 (3.08%) were from junior high school

  9. Envejecimiento en red, cauce de información sobre experiencias innovadoras

    OpenAIRE

    2015-01-01

    Póster en que se explica el objetivo del portal Envejecimiento En Red: transferir conocimiento sobre envejecimiento y personas mayores, ofreciendo información sobre la investigación que se realiza en los campos de la Gerontología y la Geriatría y sobre recursos de utilidad para profesionales de los servicios sociales y para la sociedad en general.

  10. Apuntes para un debate sobre el impacto cognoscitivo de la televisión

    OpenAIRE

    Orozco-Gómez, Guillermo

    1986-01-01

    Artículo que presenta un análisis sobre el impacto cognoscitivo de la televisión y que sustenta la tesis de que la falta de conocimiento sobre el impacto negativo de este medio es consecuencia de la forma en que los investigadores han abordado el tema. Se habla sobre el libro “Mind and media. The effects of TV, videogames and computers” de Patricia Marks Greenfield, como un buen ejemplo del paradigma de investigación dominante, y porque es uno de los primeros intentos de estudio sobre los efe...

  11. Efecto del cambio climático sobre las interacciones planta-animal y sus consecuencias sobre los ecosistemas

    OpenAIRE

    Maglianesi Sandoz, Maria Alejandra; Jones Román, Gabriela

    2016-01-01

    Las comunidades ecológicas están organizadas en redes complejas de interacción donde las especies se relacionan unas con otras a través de diferentes tipos de interacciones. Las interacciones mutuamente benéficas entre plantas y sus polinizadores y dispersores de semillas tienen una gran influencia en la dinámica de la comunidad y han contribuido a generar la biodiversidad sobre la Tierra. Además, las interacciones mutualistas planta-animal son claves en proveer funciones y servicios ecosisté...

  12. Familias reconstituidas: Un estudio sobre las nuevas estructuras familiares

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    ISABEL ESPINAR FELLMANN

    2003-01-01

    Full Text Available En la mayoría de las investigaciones sobre la familia, los estudios se han centrado en las familias intactas o primeras familias. Sin embargo, es cada vez mayor el número de familias cuya configuración incluye un segundo matrimonio e hijos de una unión anterior (i.e. familias reconstituidas. En la consolidación del complejo proceso de ajuste de estas familias se han identificado unas fuentes comunes de estrés relacionadas principalmente con la formación de un nuevo sistema familiar. El objetivo de este artículo es realizar una revisión de las variables que inciden en el bienestar y/o malestar psicológico sobre una muestra de padrastros y madrastras españoles en el seno de estas nuevas configuraciones familiares. En una primera fase se realizaron, desde un diseño cualitativo, 20 entrevistas en profundidad a una muestra de padrastros y madrastras. En una segunda fase, se diseñó un cuestionario que fue respondido por 77 sujetos con la finalidad de analizar la posible relación entre alguna de las variables estudiadas.

  13. A etnografia das ruas: notas sobre estudar e ser estudado

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    Hecht, Tobias

    2002-01-01

    Full Text Available Passeando entre as notas e as lembranças de dois períodos distintos de trabalho de campo, este ensaio "impressionista" trata da dinâmica em mudança de uma relação de pesquisa com crianças de rua. Em 1992-1993, conduzi trabalho de campo para uma dissertação sobre crianças de rua no nordeste brasileiro. Retornando à região seis anos mais tarde, descobri que a maioria das crianças estudadas apenas uma fração de geração antes estavam então mortas. Estas anotações enfocam minhas conversas com um dos sobreviventes e com quem (e sobre quem estou agora escrevendo uma biografia. Esse esforço colaborativo, voltado de certo modo a reconsiderar as implicações éticas da pesquisa com crianças que levam vidas violentas e vulneráveis, levanta uma gama de novos desafios éticos, alguns dos quais inteiramente imprevistos

  14. Nuevas reflexiones sobre la Posgerontología

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    Ricardo Iacub

    2014-03-01

    Full Text Available La Posgerontología plantea una lectura epistemológica, crítica y política sobre los relatos con los que se construye el envejecimiento y la vejez desde la gerontología. En este artículo se analizará crítica y políticamente a la gerontología, tanto como un objeto cultural exótico - por su pretensión omnicomprensiva -, como por ser un campo de conocimientos cuyos presupuestos explícitos e implícitos inciden sobre su objeto de estudio. Estos factores ponen en consideración los ajustes que debe realizar todo campo de conocimiento frente a los cambios que le imprime el contexto. Así como también expresan la necesidad de dar cuenta de los efectos de la realidad que construyen en las diversas formas de saber y en las prácticas sociales.

  15. Sobre fuentes, temporalidad y escritura de la historia

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    Germán Colmenares

    1987-01-01

    Full Text Available Una exposición sobre métodos históricos no puede reducirse a una mera colección de recetas sobre la manera como debe conducirse una investigación desde el momento en que se selecciona un tema monográfico, se localizan las fuentes aprovechables y se someten a variados procedimientos críticos y cuantitativos, hasta cuando se acomete la empresa final de la presentación narrativa o analítica de los resultados. Cada uno de estos pasos ha recibido un énfasis diferente en diferentes épocas, tipificando así una escuela o toda una manera de concebir la tarea historiográfica. El primero de ellos, por ejemplo, no parece recelar mayores dificultades. Sin embargo, cuando en él se considera incluido el planteamiento de un problema y la identificación de los elementos que lo constituyen, entonces resulta encerrar el mayor número de cuestiones teóricas.

  16. Percepções de adolescentes sobre sua sexualidade

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    Kelly Ribeiro de Freitas

    2010-01-01

    Full Text Available Es un estudio cualitativo de naturaleza descriptiva, con el objetivo de conocer las percepciones de los adolescentes sobre el desarrollo de su sexualidad. Se utilizó el enfoque del método creativo y sensible que presenta una alternativa a la investigación en enfermería a través de la dinámica de grupos de "creatividad y sensibilidad". Los participantes del estudio son 12 adolescentes de una escuela estatal de Porto Alegre. Para la recolección de los datos se hicieron cuatro talleres. El análisis siguió las etapas del método en el contexto de la pedagogía crítica de Paulo Freire. Tres temas emergieron del análisis: el adolescente: conflictos y dificultades de vida; la sexualidad para los adolescentes; y el acto sexual. Se considera importante hacer hincapié en que las percepciones de los adolescentes sobre su sexualidad surgieron en los debates y la alianza de conocimientos durante las reuniones, lo que contribuyó a la creación de un nuevo ambiente de diálogo en el contexto escolar que promovió y ayudó a la profundización del tema en un lenguaje accesible, en colaboración con los adolescentes.

  17. Preguntas y consideraciones sobre anestesia en el anciano

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    Evangelina Dávila Cabo de Villa

    2011-08-01

    Full Text Available El avance en los conocimientos sobre la fisiopatología de las enfermedades, apoyado por los adelantos científicos ha hecho posible la realización de mayor número de procedimientos quirúrgicos y con más complejidad, bajo la aplicación de métodos anestésicos y con calidad en los desempeños. El empleo juicioso de fármacos, la actualización tecnológica, la preocupación por crear mayores beneficios en los enfermos, conforman objetivos fundamentales en la anestesiología, sobre todo en ancianos. Estos pacientes exigen individualización en la realización de las estrategias terapéuticas, por lo que el presente folleto tiene como objetivo, a través de la realización de preguntas, invitar a la ejercitación mental y reflexión, contribuir al completamiento de ideas que ayuden a optimizar la asistencia a los ancianos.

  18. Percepções da adolescente sobre a maternidade

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    Janice Regina Rangel Porto

    2002-08-01

    Full Text Available O presente estudo tem por objetivo conhecer as percepções da mãe adolescente sobre a maternidade nesta etapa da vida; conhecer a percepção das adolescentes sobre o atendimento de saúde prestado pela equipe hospitalar; e conhecer o modo como gostariam de ser cuidadas no periodo de maternidade. Trata-se de um estudo exploratório descritivo, com abordagem qualitativa, realizado com 11 puérperas adolescentes, na Unidade de Internação Obstétrica de um hospital-ensino. As informações foram coletadas através de entrevistas semi-estruturadas e submetidas à análise de conteúdo temático. As informações obtidas revelam quatro temas: Estou grávida! Agora, é assumir...; Vivenciando o parto; Ser mãe é... e A gente se sente à vontade com os profissionais daqui. A faia destas jovens demonstra que um atendimento humanizado è possível, revelando nesta maternidade, profissionais sensibilizados e comprometidos com a melhoria da saúde materna.

  19. sobre o que aprendem com a tevê

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    Rosália Duarte

    2006-01-01

    Full Text Available Este artículo presenta los resultados de una encuesta realizada por el Grupo de Pesquisa en Educación y Medios de Comunicación, de la Pontificia Universidad Católica de Río de Janeiro (PUC-Río, en 2004, que tuvo como objetivo describir y comprender las relaciones que los niños establecen con la televisión, desde el punto de vista de ellos. El estudio tuvo como soporte empírico textos escritos por más de cuatrocientos niños de la Región Sudeste, con edades entre 8 y 12 años, en los que ellos expresan lo que piensan sobre lo que ven en la tele. Los procedimientos analíticos de los textos de los niños fueron estructurados a partir de ejes temáticos extraídos de los mismos. Este artículo privilegia el tema “lo que los niños piensan sobre lo que aprenden con la tele”.

  20. Códigos de linguagem no discurso sobre o cinema

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    Rosemari Fagás Viégas

    2014-01-01

    Full Text Available Este trabalho consiste em um estudo sobre os códigos da linguagem cinematográfica a partir de seus significados estético, psicológico e sociológico. Adotam-se como pontos de vista teóricos a divisão tricotômica dos signos proposta por Charles S. Peirce, a revisão crítica da Teoria da Imagem, a descrição de Walter Benjamin sobre a natureza política inerente aos processos técnicos de produção fílmica e a hipótese de que as respostas às questões apontadas pela análise do cinema, realizada por Christian Metz, dependem da solução de problemas lógicos da construção de classes de significação dentro da semiótica. O método utilizado é tanto o bibliográfico quanto aquele baseado em análise de filmografia selecionada. O trabalho procura explicitar a nova classe de signos e de representações que a linguagem digital do cinema deverá produzir no futuro próximo.