WorldWideScience

Sample records for blaster kolib pci-e-le

  1. Maakera supitehas kolib Põlvast minema / Väinu Rozental

    Index Scriptorium Estoniae

    Rozental, Väinu, 1957-

    2007-01-01

    Põlvas asuva Maakera kaubamärgi all tegutseva valmistoidutehase AS-i Nordic Foods ostnud AS Põltsamaa Felix kolib tootmise Põlvast emafirmasse Põltsamaale. Lisa: Põlva supitehas elas kaks ja pool aastat

  2. SandBlaster: Reversing the Apple Sandbox

    OpenAIRE

    Deaconescu, Răzvan; Deshotels, Luke; Bucicoiu, Mihai; Enck, William; Davi, Lucas; Sadeghi, Ahmad-Reza

    2016-01-01

    In order to limit the damage of malware on Mac OS X and iOS, Apple uses sandboxing, a kernel-level security layer that provides tight constraints for system calls. Particularly used for Apple iOS, sandboxing prevents apps from executing potentially dangerous actions, by defining rules in a sandbox profile. Investigating Apple's built-in sandbox profiles is difficult as they are compiled and stored in binary format. We present SandBlaster, a software bundle that is able to reverse/decompile Ap...

  3. GapBlaster-A Graphical Gap Filler for Prokaryote Genomes.

    Directory of Open Access Journals (Sweden)

    Pablo H C G de Sá

    Full Text Available The advent of NGS (Next Generation Sequencing technologies has resulted in an exponential increase in the number of complete genomes available in biological databases. This advance has allowed the development of several computational tools enabling analyses of large amounts of data in each of the various steps, from processing and quality filtering to gap filling and manual curation. The tools developed for gap closure are very useful as they result in more complete genomes, which will influence downstream analyses of genomic plasticity and comparative genomics. However, the gap filling step remains a challenge for genome assembly, often requiring manual intervention. Here, we present GapBlaster, a graphical application to evaluate and close gaps. GapBlaster was developed via Java programming language. The software uses contigs obtained in the assembly of the genome to perform an alignment against a draft of the genome/scaffold, using BLAST or Mummer to close gaps. Then, all identified alignments of contigs that extend through the gaps in the draft sequence are presented to the user for further evaluation via the GapBlaster graphical interface. GapBlaster presents significant results compared to other similar software and has the advantage of offering a graphical interface for manual curation of the gaps. GapBlaster program, the user guide and the test datasets are freely available at https://sourceforge.net/projects/gapblaster2015/. It requires Sun JDK 8 and Blast or Mummer.

  4. Concrete Cleaning, Inc. centrifugal shot blaster: Baseline report; Summary

    International Nuclear Information System (INIS)

    1997-01-01

    The centrifugal shot blaster is an electronically operated shot-blast machine that removes layer of concrete of varying depths. Hardened steel shot propelled at a high rate of speed abrades the surface of the concrete. The depth of material removed is determined by the rate of speed the machine is traveling and the volume of shot being fired into the blast chamber. The steel shot is reused until it is pulverized to dust, which is deposited in the waste container with the concrete being removed. Debris is continually vacuumed by a large dust collection system attached to the shot blaster. The safety and health evaluation during the human factors assessment focused on two main areas: noise and dust

  5. Bowlers, ballads, bells, and blasters: Living history and fandom [symposium

    Directory of Open Access Journals (Sweden)

    Mark Soderstrom

    2011-03-01

    Full Text Available In many people's lives the disposition to create community around historical interest or reenacted historical community practices, or even just entertainment in a mythic-history setting, intersects with a related and similar interest in science fiction/fantasy literature and participation on some level in the related fandoms and social activities of SF/F. The bowlers, ballads, bells, and blasters of my title come together not just in current steampunk scenes but also in the storied and genred lives of many reenactors and fans. Or, as a friend of mine suggested when discussing this essay, "historical reenactment is the trade secret to fandom."

  6. MANAJEMEN PENGELOLAAN WAHANA REKREASI OLAHRAGA DI WISATA WATER BLASTER SEMARANG TAHUN 2013

    Directory of Open Access Journals (Sweden)

    Mochamad Syahroni Firdiansyah

    2015-02-01

    Full Text Available This study aims to determine the process and the management of existing resources in the recreational vehicle Blaster Water sports in Semarang . This study is a qualitative research methodology . Collecting data using observation , questionnaires , interviews , documents and documentation . The data analysis technique used is descriptive percentage ( DP and the method of triangulation . The results of a descriptive study using a percentage , obtained planning process including criteria results 75 % higher , organizing the results obtained 76.3 % including high criteria , the results obtained leadership criteria included a low 53.8 % , controls 83.8 % obtained results are included in the very high criteria . Human resources results obtained 68.8 % including high criteria , the results obtained infrastructure including high criteria 74.6 % , 61.0 % of funds obtained results include lower criteria , and marketing of 70.3 % obtained results including high criteria . The conclusions in this study are the sights properly implement management processes .

  7. Fine 5 kolib Kumu lavale

    Index Scriptorium Estoniae

    2006-01-01

    Kumu kunstimuuseumi auditooriumis toimub 21. veebruaril Fine 5 kaasaegse tantsu etendus "Panus". Esinevad Tiina Ollesk, Irina Pähn, žonglöör Dimitri Kruus, disainer Rain Saukas ja muusik Mattias Siitan

  8. Animasari Bushist kolib mobiilist teleekraanile

    Index Scriptorium Estoniae

    2006-01-01

    USA mobiilioperaator Amp'd Mobile mobiiliga filmitud ja levitatud animasari "Väike Bush : Ühendriikide kodanik" läheb teletootja Comedy Central kaudu ka telelevisse. Selles laadis on see esimene projekt

  9. Kodumaine mood kolib kaubanduskeskustesse / Karilin Engelbrecht

    Index Scriptorium Estoniae

    Engelbrecht, Karilin

    2010-01-01

    Tallinnas Stockmanni kaubamajas müüakse kuu aja jooksul eesti moekunstnike rõivakollektsioone. Projekti juhib Oksana Tandit. Kristiine keskuse Moetäikal müüvad eesti disainerid taaskasutatud riideid ning ReUse Republicu disainilaadal vanadest laoülejääkidest õmmeldud rõivaid

  10. Kino kolib suveks õue / Janno Zõbin

    Index Scriptorium Estoniae

    Zõbin, Janno

    2002-01-01

    Detsembris toimuvat Pimedate Ööde Filmifestivali laiendatakse ka suvele, et sobivates paikades näidata väärtfilme. Tallinnas Katariina kirikus ja Theatrumis juba näidati K. Kieslowski filmide retrospektiivi. Juunis näidatakse Werner Herzogi filme ning Tallinna Linnaarhiivi siseõues hakkab tegutsema juba traditsiooniks saanud vabaõhukino. Noortefilme näidatakse Nukuteatri hoovis

  11. Kunstiakadeemia kolib ajutiselt Estonia puiesteele / Mari Kodres

    Index Scriptorium Estoniae

    Kodres, Mari

    2009-01-01

    Eesti Kunstiakadeemia on 2010. a. alguseks vanadest ruumidest kolinud hoonesse aadressil Estonia pst. 7. Rüütelkonna hoones leidis endale ajutise kodu vabade kunstide teaduskonna enamik erialasid. Veebruari algul toimuvast tühjendamispeost. EKA uue maja arhitektuurikonkursi võitis Taani arhitektuuribüroodes Sea ja Effekt valminud töö "Art Plaza". Uue hoone ehitus algab aprillis, valmimistähtajaks on 2012. a. kevad

  12. Kokakoolakinno kolib ooper, popkorn on out / Tiit Tuumalu

    Index Scriptorium Estoniae

    Tuumalu, Tiit, 1971-

    2008-01-01

    Forum Cinemas võimaldab Tartu kinos Ekraan ja Tallinnas Coca-Cola Plazas kord kuus laupäevaõhtuti vaadata-kuulata klassikaooperite otseülekandeid Metropolitan Opera lavalt (Metropolitani projekti "The Met: Live in HD" raames)

  13. Largo mahlatööstus kolib pealinnast Võhmasse / Hans Väre

    Index Scriptorium Estoniae

    Väre, Hans, 1979-

    2007-01-01

    Jõustus Svensky Kaubanduse ja Largo endise omaniku, piimatööstuse Tere vaheline müügileping, mille järgi saab Svenksy Kaubandus endale kõik Largoga seonduva alates kaubamärgist ja seadmetest, lõpetades toodanguga. Arvamust avaldab Võhma linnapea Avo Põder

  14. Living.ee : mööblimüük kolib internetti / Kerli Nõu

    Index Scriptorium Estoniae

    Nõu, Kerli

    2006-01-01

    Internetis mööblit müüv Living.ee kavatseb 2008. aastaks saada müügimahu poolest sama suureks kui Mööblimaja või Aatrium, plaanis on müüa mööblit hulgi ka Skandinaavias. Tabel: OÜ Lagersen (Living.ee). Kommenteerib Arno Kütt

  15. Von Krahli teater kolib mõneks ajaks Saksamaale / Rain Tolk

    Index Scriptorium Estoniae

    Tolk, Rain, 1977-

    2006-01-01

    Von Krahli Teatri selle hooaja eelviimane esietendus "Europiraadid" on 17. mail Düsseldorfis, koostöös Saksamaa teatrirühmaga Showcase Beat Le Mot. Eesti poole pealt teevad kaasa Juhan Ulfsak, Taavi Eelmaa, Maarja Jakobson ja üllatusmängijana filmimees Rain Tolk, kes räägib oma suhtumisest teatrisse ja suhetest Von Krahli Teatriga

  16. New seismic source `BLASTER` for seismic survey; Hasaiyaku wo shingen to shite mochiita danseiha tansa

    Energy Technology Data Exchange (ETDEWEB)

    Koike, G; Yoshikuni, Y [OYO Corp., Tokyo (Japan)

    1996-10-01

    Built-up weight and vacuole have been conceived as seismic sources without using explosive. There have been problems that they have smaller energy to generate elastic wave than explosive, and that they have inferior working performance. Concrete crushing explosive is tried to use as a new seismic source. It is considered to possess rather large seismic generating energy, and it is easy to handle from the viewpoint of safety. Performance as seismic source and applicability to exploration works of this crushing explosive were compared with four kinds of seismic sources using dynamite, dropping weight, shot-pipe utilizing shot vacuole, and impact by wooden maul. When considered by the velocity amplitude, the seismic generating energy of the crushing explosive of 120 g is about one-fifth of dynamite of 100 g. Elastic wave generated includes less high frequency component than that by dynamite, and similar to that using seismic source without explosive, such as the weight dropping. The maximum seismic receiving distance obtained by the seismic generation was about 100 m. This was effective for the slope survey with the exploration depth between 20 m and 30 m. 1 ref., 9 figs., 2 tabs.

  17. Microsofti sõda Googleþi vastu / Jay Greene

    Index Scriptorium Estoniae

    Greene, Jay

    2008-01-01

    Microsofti konkurentsist Googleþiga, ettevõtte uuest strateegiast saavutada ülekaal internetireklaamiturul. Graafik. Lisad: Microsofti reklaamiimperatiiv; Veebireklaami turg; Tarkvara kolib veebi; Google'i eduseis; Reklaamimaht kasvab

  18. Kultuuritehas vallutab vana katlamaja / Merit Kask

    Index Scriptorium Estoniae

    Kask, Merit

    2005-01-01

    Kultuuritehase projektijuht Madis Mikkor Tallinna Põhja puiesteel asuva vana elektrijaama tööstusmiljööst ja hoonetest, kuhu kolib kultuuritehas Polymer. Katlamaja plaanitakse avada augustis mitmepäevase festivaliga

  19. Karastusjoogihiid võib Eestile selja pöörata / Virge Lahe, Artur Tooman

    Index Scriptorium Estoniae

    Lahe, Virge

    2008-01-01

    Coca-Cola kolib kulude optimeerimiseks oma tootmise Tallinnast Lätti. Vt. samas: Õlletehas jätkab Sakus; Farmaatsiatehas võib kolida Riiga; Coca-Cola Hellenic. Diagramm: Coca-Cola majandusnäitajad

  20. Väljak: siseväljak = Square: inner plaza / Kristi Tuurmann, Tiia Vahula, Riin Kersalu, Kerstin Kivila

    Index Scriptorium Estoniae

    Tuurmann, Kristi

    2013-01-01

    EKA arhitektuuri ja linnaplaneerimise osakonna III kursuse 2009-2012 aastail erialase projekteerimise raames loodud lahendus, kus ei teki raekoja ette traditsioonilist väljakut, pigem "kolib" see avaliku ruumina linnavalitsuse sisse

  1. Leedu päritolu kasiinoärimees püsib rikkuselt kolmanda ameeriklasena / Erik Aru

    Index Scriptorium Estoniae

    Aru, Erik

    2006-01-01

    Väidetavalt Leedu päritolu kasiinoärimehest Sheldon Adelsonist, tema rivaliteedist Steve Wynniga, nende tegevus Hiina Rahvavabariigi territooriumil Macaos. Lisa: Las Vegas kolib Aasiasse. Tabel : Las Vegas Sands Corporation

  2. Moroz i rozõ / Lev Livshits

    Index Scriptorium Estoniae

    Лившиц, Лев, 1924-2013

    2006-01-01

    Ain Järvega Kadrioru pargist. Kadrioru Lastepargi tekkimise ajaloost ja uue Lastepargi rajamisest. Ehitatakse uus Lastemuuseumi hoone kuhu kolib Kotzebue tänavalt Tallinna Nukumuuseum. Nukumuuseumist ja muuseumidest Kadriorus, ka KUMU hoonest

  3. Kulturu delajut na zavodah / Alina Zelimhanova

    Index Scriptorium Estoniae

    Zelimhanova, Alina

    2005-01-01

    Kultuuritehase projektijuht Madis Mikkor Tallinnas Põhja puiesteel asuvast muinsuskaitse all olevast elektrijaama katlamajast, kuhu peatselt kolib kultuuritehas Polymer. Katlamaja plaanitakse avada augustis. Selleks ajaks seatakse sisse valgustus. Hoonete põhjalikum renoveerimine algab 2007. a., kusjuures säilitatakse vana tööstusmiljöö

  4. 30 CFR 850.14 - Examination.

    Science.gov (United States)

    2010-07-01

    ... 30 Mineral Resources 3 2010-07-01 2010-07-01 false Examination. 850.14 Section 850.14 Mineral..., EXAMINATION, AND CERTIFICATION OF BLASTERS PERMANENT REGULATORY PROGRAM REQUIREMENTS-STANDARDS FOR CERTIFICATION OF BLASTERS § 850.14 Examination. (a) The regulatory authority shall ensure that candidates for...

  5. Popmuusika / Aimar Ventsel

    Index Scriptorium Estoniae

    Ventsel, Aimar, 1970-

    2006-01-01

    Uutest heliplaatidest Amparanoia "La Vida Te Da", "Kui lähed, mine iseendasse", Cyantific "Ghetto Blaster", The Like "Are You Thinkingb What I'm Thinking?", Prince "3121", Lordi "The Arockalypse", "Twisted Disco"

  6. Software Reviews. Programs Worth a Second Look.

    Science.gov (United States)

    Schneider, Roxanne; Eiser, Leslie

    1989-01-01

    Reviewed are three computer software packages for use in middle/high school classrooms. Included are "MacWrite II," a word-processing program for MacIntosh computers; "Super Story Tree," a word-processing program for Apple and IBM computers; and "Math Blaster Mystery," for IBM, Apple, and Tandy computers. (CW)

  7. How To: Protect against a Zero-Hour Attack

    Science.gov (United States)

    McCarthy, Rob

    2005-01-01

    In the last year, a series of viruses and worms that caused damage across the Internet in record time has made very clear how vulnerable computer systems are. The MS Blaster, Slammer, Sasser, and Korgo.W worms have shown that signature-based antivirus software and traditional firewalls are not enough to protect networks. Everyone is worried about…

  8. 75 FR 60371 - Alabama Regulatory Program

    Science.gov (United States)

    2010-09-30

    ..., excluding holidays. You may receive one free copy of the amendment by contacting OSM's Birmingham Field... continuing education of certified blaster applicants. D. Alabama Code Sec. 9-16-77(b) Amends existing... public hearing will continue on the specified date until everyone scheduled to speak has been given an...

  9. Automated docking screens: a feasibility study.

    Science.gov (United States)

    Irwin, John J; Shoichet, Brian K; Mysinger, Michael M; Huang, Niu; Colizzi, Francesco; Wassam, Pascal; Cao, Yiqun

    2009-09-24

    Molecular docking is the most practical approach to leverage protein structure for ligand discovery, but the technique retains important liabilities that make it challenging to deploy on a large scale. We have therefore created an expert system, DOCK Blaster, to investigate the feasibility of full automation. The method requires a PDB code, sometimes with a ligand structure, and from that alone can launch a full screen of large libraries. A critical feature is self-assessment, which estimates the anticipated reliability of the automated screening results using pose fidelity and enrichment. Against common benchmarks, DOCK Blaster recapitulates the crystal ligand pose within 2 A rmsd 50-60% of the time; inferior to an expert, but respectrable. Half the time the ligand also ranked among the top 5% of 100 physically matched decoys chosen on the fly. Further tests were undertaken culminating in a study of 7755 eligible PDB structures. In 1398 cases, the redocked ligand ranked in the top 5% of 100 property-matched decoys while also posing within 2 A rmsd, suggesting that unsupervised prospective docking is viable. DOCK Blaster is available at http://blaster.docking.org .

  10. 30 CFR 955.16 - Reciprocity.

    Science.gov (United States)

    2010-07-01

    ... 30 Mineral Resources 3 2010-07-01 2010-07-01 false Reciprocity. 955.16 Section 955.16 Mineral... AND ON INDIAN LANDS § 955.16 Reciprocity. (a) Grant of certificate. OSM shall grant an OSM blaster certificate through reciprocity to any qualified applicant who demonstrates that he or she, and whom OSM finds...

  11. Kirev kamp / Harri Slip

    Index Scriptorium Estoniae

    Slip, Harri

    2015-01-01

    Bluetooth-kõlarid hinnaklassist 150-200 €: Beats Speaker Pill 2.0, Bose SoundLink Mini, Cambridge Audio GoRadio, Creative Sound Blaster Roar, Denon Envaya, Jabra Solemate, JBL Charge 2, SMS Audio SYNCby 50, Sony SRS-X5, TDK Trek Max A34, Ultimate Ears Boom

  12. Storage stability of flour-blasted brown rice

    Science.gov (United States)

    Brown rice was blasted with rice flour rather than sand in a sand blaster to make microscopic nicks and cuts so that water can easily penetrate into the brown rice endosperm and cook the rice in a shorter time. The flour-blasted American Basmati brown rice, long grain brown rice, and parboiled long...

  13. Long-Term Performance Evaluation of Asphalt Surface Treatments: Product Placement

    Science.gov (United States)

    2010-02-01

    spreader . The aggregate was applied to increase the friction of the treated area. A Ford F550 truck was used to seat the aggregate by traversing the...area is sealed, and the steel media are vacuumed back through the shot blaster to be recycled and cleaned for repetitive use. Once the process is...applying the rejuvenator, approximately 0.7 lb/yd2 of Black Beauty aggregate was spread over the section using a garden fertilizer spreader . The

  14. Avoiding tragedy: lessons to be learned from a flyrock fatality

    Energy Technology Data Exchange (ETDEWEB)

    Shea, C.W.; Clark, D. [US Department of the Interior, Knoxville, TN (United States). Office of the Field Solicitor

    1998-02-01

    The reasons for a fatality from fly rock at a Tennessee surface coal mine are discussed. Blast design was inadequate, and safety regulations were broken. The results of a US Office of Surface Mining report on this incident are discussed. Blast design had been changed without the possible consequences being considered. The blaster was imprisoned and the company had to pay exemplary damages. Blasting should not be done unless its precise effects can be calculated. 5 refs., 2 figs.

  15. You've got 'scale' : developments in well-bore remediation technology

    Energy Technology Data Exchange (ETDEWEB)

    Zemlak, Z. [Schlumberger Canada Ltd., Calgary, AB (Canada); Kortash, B. [Amoco Canada Petroleum Co. Ltd., Calgary, AB (Canada)

    2000-06-01

    The Kaybob field Beaver Hill Lake formation in Central Alberta is an aquifer driven maturing gas reservoir with high concentrations of iron sulfide scale. This scale, in combination with a time layered asphaltenes has an impact on production. Since the scale forms on tubular walls and results in corrosion of metal, it also increases risks during well intervention. Past techniques to remove the scale have not proven to be successful. This study presents a newly developed Blaster scale removal system to clean tubings. Blaster techniques use high-pressure jetting in combination with special abrasives to remove extremely hard, inert scales without damaging the tubing or completion components. Amoco Canada has been involved in the continual improvement and modification of the method since its introduction in 1998. To date, a total of 19 wells have been completed, providing valuable data about the method. It was concluded that although iron sulfide scale is common throughout the gas reservoir, the composition and solubility varies from well to well. Amoco Canada has found that Blaster techniques are a safe, low risk method of cleaning coiled tubulars to known drift parameters, in areas where scale and corrosion have been uncontrollable. 5 refs., 2 tabs., 1 fig.

  16. Surface impurity removal from DIII-D graphite tiles by boron carbide grit blasting

    International Nuclear Information System (INIS)

    Lee, R.L.; Hollerbach, M.A.; Holtrop, K.L.; Kellman, A.G.; Taylor, P.L.; West, W.P.

    1993-11-01

    During the latter half of 1992, the DIII-D tokamak at General Atomics (GA) underwent several modifications of its interior. One of the major tasks involved the removal of accumulated metallic impurities from the surface of the graphite tiles used to line the plasma facing surfaces inside of the tokamak. Approximately 1500 graphite tiles and 100 boron nitride tiles from the tokamak were cleaned to remove the metallic impurities. The cleaning process consisted of several steps: the removed graphite tiles were permanently marked, surface blasted using boron carbide (B 4 C) grit media (approximately 37 μm. diam.), ultrasonically cleaned in ethanol to remove loose dust, and outgassed at 1000 degrees C. Tests were done using, graphite samples and different grit blaster settings to determine the optimum propellant and abrasive media pressures to remove a graphite layer approximately 40-50 μm deep and yet produce a reasonably smooth finish. EDX measurements revealed that the blasting technique reduced the surface Ni, Cr, and Fe impurity levels to those of virgin graphite. In addition to the surface impurity removal, tritium monitoring was performed throughout the cleaning process. A bubbler system was set up to monitor the tritium level in the exhaust gas from the grit blaster unit. Surface wipes were also performed on over 10% of the tiles. Typical surface tritium concentrations of the tiles were reduced from about 500 dpm/100 cm 2 to less than 80 dpm/100 cm 2 following the cleaning. This tile conditioning, and the installation of additional graphite tiles to cover a high fraction of the metallic plasma facing surfaces, has substantially reduced metallic impurities in the plasma discharges which has allowed rapid recovery from a seven-month machine opening and regimes of enhanced plasma energy confinement to be more readily obtained. Safety issues concerning blaster operator exposure to carcinogenic metals and radioactive tritium will also be addressed

  17. Pro Android Games

    CERN Document Server

    Silva, Vladimir

    2010-01-01

    Do you remember landmark games like Wolfenstein 3D, Doom, and Asteroids? Well, here's an exciting opportunity to build and/or port these games to one of the hottest mobile and netbooks platforms today: Google's Android. Pro Android Games teaches you how to build cool games like Space Blaster and the classic Asteroids from scratch on the latest Android platform. This book also shows you how to port other classic freeware/shareware games like Doom and Wolfenstein 3D from C using the Java Native Interface (JNI) for Android. This book is all about a unique perspective in Android game development:

  18. Arbejde med sandblæsning kan give lungesilikose

    DEFF Research Database (Denmark)

    Schelde, Jacob; Authried, Georg; Madsen, Helle Dall

    2015-01-01

    Silicosis is a common occupational disease worldwide. It is caused by the inhalation of crystalline silicon dioxide, i.e. silica. Quartz is a common form of silica and occurs in sandstone and granite. Occupational exposure can occur e.g. in mining, quarrying and sandblasting. The inhaled silica t...... triggers an inflammatory response when phagocytosed which eventually causes fibrosis. We present a 45-year-old male Danish sand-blaster who developed silicosis, and due to rapid decline in lung function received a lung transplant with an excellent result....

  19. The Decontamination Technique for Metal by Using Pressured Solid of CO2

    International Nuclear Information System (INIS)

    Sutoto

    1998-01-01

    The decontamination of radioactive deposited in the stainless steel 304l type has been done. The radiation exposure was reduced from 460 to 200 mrem/h. For the supporting of the processes, the compressed air 12 bar was flue in blaster. The various of treatment are blasting time between 15 to 60 seconds. Result of the experiment is decontamination factor (DF) = 0,22 and the consumption of dry ice is 10 kgs needed. The secondary waste generated as aerosol were treated by HEPA Filter

  20. Experimental Study of Removing Surface Corrosion Products from Archaeological Iron Objects and Alternating Iron Corrosion Products by Nd:YAG Laser Cleaning System

    Energy Technology Data Exchange (ETDEWEB)

    Lee, Hye Youn; Cho, Nam Chul [Kongju National University, Daejeon (Korea, Republic of); Lee, Jong Myoung [IMT co. Ltd, Suwon (Korea, Republic of); Yu, Jae Eun [National Research Institute of Cultural Heritage, Daejeon (Korea, Republic of)

    2012-05-15

    The corrosion product of archaeological iron objects is supposed to be removed because it causes re-corrosion. So far it is removed by scapel and sand blaster but they depend on the skill and experience of a conservator and the glass-dust of the sand blaster is harmful to humans. Therefore this study applies a laser cleaning system which is used in various industrial cleaning processes, to remove corrosion product from archaeological iron objects. In addition, this work studies the alternation of corrosion product after laser irradiation, which evaluates the reliability of the laser cleaning system. Optical microscopy, SEM-EDS, XRD, Raman have been used to observe and analyse the surface of the objects. The results show the capacity of laser cleaning some corrosion product, but blackening appears with increasing pulses and laser energy, and some corrosion products, goethite and hematite, are partially altered to magnetite. These problems, blackening and alternation of corrosion product, should be solved by further studies which find the optimal laser irradiation condition and use a wetting agent.

  1. Debilitating lung disease among surface coal miners with no underground mining tenure.

    Science.gov (United States)

    Halldin, Cara N; Reed, William R; Joy, Gerald J; Colinet, Jay F; Rider, James P; Petsonk, Edward L; Abraham, Jerrold L; Wolfe, Anita L; Storey, Eileen; Laney, A Scott

    2015-01-01

    To characterize exposure histories and respiratory disease among surface coal miners identified with progressive massive fibrosis from a 2010 to 2011 pneumoconiosis survey. Job history, tenure, and radiograph interpretations were verified. Previous radiographs were reviewed when available. Telephone follow-up sought additional work and medical history information. Among eight miners who worked as drill operators or blasters for most of their tenure (median, 35.5 years), two reported poor dust control practices, working in visible dust clouds as recently as 2012. Chest radiographs progressed to progressive massive fibrosis in as few as 11 years. One miner's lung biopsy demonstrated fibrosis and interstitial accumulation of macrophages containing abundant silica, aluminum silicate, and titanium dust particles. Overexposure to respirable silica resulted in progressive massive fibrosis among current surface coal miners with no underground mining tenure. Inadequate dust control during drilling/blasting is likely an important etiologic factor.

  2. Keeping condensers clean

    Energy Technology Data Exchange (ETDEWEB)

    Wicker, K.

    2006-04-15

    The humble condenser is among the biggest contributors to a steam power plant's efficiency. But although a clean condenser can provide great economic benefit, a dirty one can raise plant heat rate, resulting in large losses of generation revenue and/or unnecessarily high fuel bills. Conventional methods for cleaning fouled tubes range form chemicals to scrapers to brushes and hydro-blasters. This article compares the available options and describes how one power station, Omaha Public Power District's 600 MW North Omaha coal-fired power station, cleaned up its act. The makeup and cooling water of all its five units comes from the Missouri River. 6 figs.

  3. Development and evaluation of a cleanable high efficiency steel filter

    International Nuclear Information System (INIS)

    Bergman, W.; Larsen, G.; Weber, F.; Wilson, P.; Lopez, R.; Valha, G.; Conner, J.; Garr, J.; Williams, K.; Biermann, A.; Wilson, K.; Moore, P.; Gellner, C.; Rapchun, D.; Simon, K.; Turley, J.; Frye, L.; Monroe, D.

    1993-01-01

    We have developed a high efficiency steel filter that can be cleaned in-situ by reverse air pulses. The filter consists of 64 pleated cylindrical filter elements packaged into a 6l0 x 6l0 x 292 mm aluminum frame and has 13.5 m 2 of filter area. The filter media consists of a sintered steel fiber mat using 2 μm diameter fibers. We conducted an optimization study for filter efficiency and pressure drop to determine the filter design parameters of pleat width, pleat depth, outside diameter of the cylinder, and the total number of cylinders. Several prototype cylinders were then built and evaluated in terms of filter cleaning by reverse air pulses. The results of these studies were used to build the high efficiency steel filter. We evaluated the prototype filter for efficiency and cleanability. The DOP filter certification test showed the filter has a passing efficiency of 99.99% but a failing pressure drop of 0.80 kPa at 1,700 m 3 /hr. Since we were not able to achieve a pressure drop less than 0.25 kPa, the steel filter does not meet all the criteria for a HEPA filter. Filter loading and cleaning tests using AC Fine dust showed the filter could be repeatedly cleaned by reverse air pulses. The next phase of the prototype evaluation consisted of installing the unit and support housing in the exhaust duct work of a uranium grit blaster for a field evaluation at the Y-12 Plant in Oak Ridge, TN. The grit blaster is used to clean the surface of uranium parts and generates a cloud of UO 2 aerosols. We used a 1,700 m 3 /hr slip stream from the 10,200 m 3 /hr exhaust system

  4. Personality, depression and problematic internet use

    Directory of Open Access Journals (Sweden)

    Viktória Kopuničová

    2016-01-01

    Full Text Available Problematic internet use (PIU is considered as a relatively new area of risk taking behaviour, which deals with uncontrolled use of the Internet with negative outcomes (impact on individuals (Caplan, 2010. This paper focuses on relationship between personality factors, depression and problematic internet use among students of secondary schools and universities in Ostrava region. The aim of the paper was to determine whether personality factors and depression predict problematic internet use among young students. Studies dealing with problematic internet use (Caplan, 2010; Young 1998; Davis, 2001 etc. show that personality is one of the factor which may be associated with internet addiction or other forms of risk behaviour (Kolibáš, Novotný, 1996; Kopasová, 2000; Hemochová, Vaňková & Drlíková in Výrost & Slameník, 2001. Personality was measured by the questionnaire HEXACO (Ashton & Lee, 2009, depression was measured by a modified version of Beck Depression Inventory (M-BDI; Schmitt, Beckmann, Dusi, Maes, Schiller &Schonauer, 2003, the problematic internet use was measured by Generalized problematic internet use scale (GPIUS2; Caplan, 2010. The research sample consisted of 279 students of secondary schools and universities in Ostrava region. There were 200 (71.7% high school students while the number of the university students was 79 (28.3%. The mean age of the sample was M = 18,5 years, SD = 2,73 and 79,9% were women. The results of Pearson correlation coefficients showed a positive relationship between depression, emotionality and PIU. Between the personality factors honesty-humility, extroversion and conscientiousness was a negative relationship with the PIU. Results of the regression analysis showed four factors - conscientiousness, depression, honesty-humility and emotions that explain 26% of the variance of the Problematic internet use among our research sample. The results confirm the importance of examining personality factors

  5. The bonding of protective films of amorphic diamond to titanium

    Science.gov (United States)

    Collins, C. B.; Davanloo, F.; Lee, T. J.; Jander, D. R.; You, J. H.; Park, H.; Pivin, J. C.

    1992-04-01

    Films of amorphic diamond can be deposited from laser plasma ions without the use of catalysts such as hydrogen or fluorine. Prepared without columnar patterns of growth, the layers of this material have been reported to have ``bulk'' values of mechanical properties that have suggested their usage as protective coatings for metals. Described here is a study of the bonding and properties realized in one such example, the deposition of amorphic diamond on titanium. Measurements with Rutherford backscattering spectrometry and transmission electron microscopy showed that the diamond coatings deposited from laser plasmas were chemically bonded to Ti substrates in 100-200-Å-thick interfacial layers containing some crystalline precipitates of TiC. Resistance to wear was estimated with a modified sand blaster and in all cases the coating was worn away without any rupture or deterioration of the bonding layer. Such wear was greatly reduced and lifetimes of the coated samples were increased by a factor of better than 300 with only 2.7 μm of amorphic diamond.

  6. Summary review of Mound Facility's experience in decontamination of concrete

    International Nuclear Information System (INIS)

    Combs, A.B.; Davis, W.P.; Garner, J.M.; Geichman, J.R.

    1980-01-01

    Most of the current concrete decontamination work at Mound Facility involves surfaces that are contaminated with plutonium-238. Approximately 60,000 sq. ft. of concrete floors will have to be decontaminated in Mound's current Decontamination and Decommissioning (D and D) Project. Although most of these surfaces are partially protected by a barrier (tile or paint), contaminated water and acid have penetrated these barriers. The technique for decontaminating these floors is desribed. The initial cleaning of the floor involes standard water and detergent. Acids are not used in cleaning as they tend to drive the contamination deeper into the concrete surface. Next, the floor tile is manually removed inside a temporary enclosure under negative and filtered ventilation. Finally, layers of contaminated concrete are mechanically removed inside the ventilated enclosure. The suspected depth and surface area of contamination determines the type of mechanical tool used. In summary, several generic methods of concrete decontamination can be utilized: chemical, such as water, detergent, acids, paint remover, strippable paints, etc.; rotary using sanders, grinders, scarifiers, etc.; impact such as pressure washers (hydrolasers), particle blasters, scabblers, needlers, spallers, paving and rock breakers, ram hoes, etc. The particular method used depends on several factors: surface and area involved; depth of contamination; cost and availability of equipment; usage safety and radiological control; and waste generated

  7. The second generation of electronic blasting systems

    Energy Technology Data Exchange (ETDEWEB)

    Hammelmann, F.; Petzold, J. [Dynamit Nobel GmbH (Germany)

    2001-07-01

    8 years after the market introduction of the first commercial electronic detonator - DYNATRONIC - the paper describes a new area of electronic blasting systems Made in Germany: i-kon. The results of a joint development between Dynamit Nobel and Orica is a unique universal electronic detonator, which is as simple to use as a standard non-electric detonator. The delay time or delay interval is not factory preprogrammed and the system is not based on a numbered system like conventional detonators. The miner or Blaster decides on site which delay timing he likes to use and is programming the whole blast on site. The new i-kon system allows delay times between 0 and 8000 ms by increments of 1 ms. With the control equipment it is possible to blast up to 1600 detonators in a single blast. The paper describes the construction and functionality of this new electronic blasting system - manufactured and developed by Precision Blasting Systems, a joint venture between Orica and Dynamic Nobel. (orig.)

  8. [Analysis on occupational noise-induced hearing loss of different type workers in underground mining].

    Science.gov (United States)

    Liu, Q C; Duo, C H; Wang, Z; Yan, K; Zhang, J; Xiong, W; Zhu, M

    2017-11-20

    Objective: To investigate hearing loss status of blasters, drillers mechanics and so on in underground mining, and put forward suggestion diagnosis of occupational explosive deafness and occupational deafness. Methods: Underground excavation workers in a metal mine were recruited in this study, those with a history of ear disease and non-occupational deafness were all excluded. Finally, the features of pure tone audiometry of 459 noise-exposed workers were analyzed. Results: High-frequency hearing loss occurred on 351workers and the positive detection rate was 74.29%, workers who had both high-frequency and linguistic frequency hearing loss were 51 and the positive detection rate was 11.11%. The positive detection of high-frequency hearing loss in right ear (χ(2)=9.427 and P = 0.024) and in left ear (χ(2)=14.375, P =0.002) was significantly different between different exposure age groups. The positive detection of high-frequency hearing loss of driving group was the highest, followed by blasting group, mining group and machine repair group. The characteristics of the hearing loss caused by drilling noise of the blasting workers with no accident occurred were in line with that of noise-induced hearing loss. Conclusion: The diagnosis grading should be carried out according to the diagnostic criteria of occupational noise-induced deafness for the employees who engaged in the blasting operation with no record of blast accident.

  9. Cleaning of road accessories with powdered sodium bicarbonate; Jutansan natrium ryu wo mochiita doro fuzokubutsu no senjo

    Energy Technology Data Exchange (ETDEWEB)

    Yamashita, H.; Amagori, Y.; Mori, E. [Japan Highway Public Corporation, Tokyo (Japan)

    1998-06-25

    A method for cleaning highway accessories with powdered sodium bicarbonate is developed, to replace mechanical cleaning by a multi-purpose vehicle or manual cleaning, and thereby to reduce road maintenance cost. It pneumatically blows sodium bicarbonate particles by a blaster connected to a water tank and compressor onto a variety of stains to remove them. The cleaning system is applicable to a variety of purposes, allowing dry and wet cleaning modes to be easily switched from each other. Sodium bicarbonate, being naturally occurring, is harmless, and its type can be freely chosen. This method was applied in FY1996 mainly to cleaning interior plates in highways, to collect the basic data. Its cleaning effectiveness has been confirmed by applying it to the internal plates, which are difficult to clean by a multi-purpose vehicle, and illuminators in tunnels, whose cleaning areas are narrow. It recovers reflectivity of plates to 85% of the initial level at the highest, and shows 3 times higher efficiency for heavily stained plates. The test was continued in FY1997. It shows an approximately 5 times higher cleaning capacity than the conventional method, greatly reducing cleaning time, especially for illuminators in tunnels. 7 figs., 5 tabs.

  10. Review of World of Money CD-ROM for PC/Mac [CD-ROM

    Directory of Open Access Journals (Sweden)

    Philip de Jersey

    1998-12-01

    Full Text Available Following on from the impressive development of the new HSBC Money Gallery in 1997, the British Museum has launched into the world of electronic publishing with the World of Money, an "interactive exploration of money worldwide from ancient times to the present day". Intended for ages ten to adult, the CD promises "a mine of information about the use, form, history and importance of money around the globe", and "fun and information for all the family". Reviewing in a Mac-unfriendly environment, I have been able to run the CD only on a PC. On a 166Mhz MMX with 32Mb RAM and a 12x CD it runs impressively quickly, with no more than two or three seconds of the "loading new page" display when switching between different parts of the program. Minimum requirements are listed as a 486 with 8Mb RAM, 40Mb free hard disk space, Windows 3.11 or Windows 95, 4x CD drive, 1Mb 256 colours graphics card, 16-bit Sound Blaster compatible sound card, and a VGA monitor. Minimum requirements for the Mac are listed as System 7, 603e processor, 16Mb RAM, 40MB free hard disk space, 6x CD drive, 1Mb video card and Apple monitor or Multisync with adaptor. The CD opens with an image of the British Museum portico, through which we are taken into the foyer, complete with the sound effects of massed ranks of tourists. We have four options available on a lectern, or by turning left, right or going upstairs: History of Money, Information Centre, Activities, and Options. Clicking on a large question mark brings up the Help screens (Figure 1.

  11. Reconnaissance of Archaeological Sites at Tuzkhormato Region Saladdin Governorate – Northern Iraq

    Directory of Open Access Journals (Sweden)

    Salman Z. Khorshid

    2018-05-01

    Full Text Available This study is talking for the first time about in this region to document the archaeological sites at Tuzkhormato and surroundings. There are more than (19 archaeological sites which took two trends  one of them is parallel to Aq-Su river, which starts from behind Tuzkhomato mountain and takes NE-SW direction at Shirash Tapa site and ending at Zeghaitoon river near the bridge of (Tikrit-Tuz road, and another trend is perpendicular to the first one at about the mid-way distance of the first trend. Coordinations of (5 archaeological sites were located and studied, these sites are as a hill ranges in its shape between small to large and the broken pottery were spread widely on the surface of these sites and some of this pottery are colored, also there are chert cutter that is used as knives (at Tal-Al-Shaer site only. These sites were uplifted from the surface level with uneven degree depending on the volume of the buildings at that site, and all the buildings were built from muds, so these buildings are weak to resist the climate. It is probable that all buildings were buried under these sites, and the destroyed pottery of pre-Islamic period was distributed over the archaeological sites. Only one site on the Tuzkhormato mountain built with blaster and gypsum rocks, this site is (Gawor Qalasi which from (Sassanian period. All these sites still are not studied geologically or geophysically for determination the ages of these sites or delineating the geometrical shapes of buried buildings or searching some of the precious things like gold or other things.

  12. Study of wet blasting of components in nuclear power stations

    International Nuclear Information System (INIS)

    Hall, J.

    1999-12-01

    This report looks at the method of wet blasting radioactive components in nuclear power stations. The wet blaster uses pearl shaped glass beads with the dimensions of 150-250 μm mixed with water as blasting media. The improved design, providing outer operator's positions with proper radiation protection and more efficient blasting equipment has resulted in a lesser dose taken by the operators. The main reason to decontaminate components in nuclear power plants is to enable service on these components. On components like valves, pump shafts, pipes etc. oxides form and bind radiation. These components are normally situated at some distance from the reactor core and will mainly suffer from radiation from so called activation products. When a component is to be decontaminated it can be decontaminated to a radioactive level where it will be declassified. This report has found levels ranging from 150-1000 Bq/kg allowing declassification of radioactive materials. This difference is found between different countries and different organisations. The report also looks at the levels of waste generated using wet blasting. This is done by tracking the contamination to determine where it collects. It is either collected in the water treatment plant or collected in the blasting media. At Barsebaeck the waste levels, from de-contaminating nearly 800 components in one year, results in a waste volume of about 0,250 m 3 . This waste consists of low and medium level waste and will cost about 3 600 EURO to store. The conclusions of the report are that wet blasting is an indispensable way to treat contaminated components in modern nuclear power plants. The wet blasting equipment can be improved by using a robot enabling the operators to remotely treat components from the outer operator's positions. There they will benefit from better radiation protection thus further reduce their taken dose. The wet blasting equipment could also be used to better control the levels of radioactivity on

  13. Preliminary field evaluation of high efficiency steel filters

    Energy Technology Data Exchange (ETDEWEB)

    Bergman, W.; Larsen, G.; Lopez, R. [Lawrence Livermore National Laboratory, CA (United States)] [and others

    1995-02-01

    We have conducted an evaluation of two high efficiency steel filters in the exhaust of an uranium oxide grit blaster at the Y-12 Plant in Oak Ridge Tennessee. The filters were installed in a specially designed filter housing with a reverse air-pulse cleaning system for automatically cleaning the filters in-place. Previous tests conducted on the same filters and housing at LLNL under controlled conditions using Arizona road dust showed good cleanability with reverse air pulses. Two high efficiency steel filters, containing 64 pleated cartridge elements housed in the standard 2` x 2` x 1` HEPA frame, were evaluated in the filter test housing using a 1,000 cfm slip stream containing a high concentration of depleted uranium oxide dust. One filter had the pleated cartridges manufactured to our specifications by the Pall Corporation and the other by Memtec Corporation. Test results showed both filters had a rapid increase in pressure drop with time, and reverse air pulses could not decrease the pressure drop. We suspected moisture accumulation in the filters was the problem since there were heavy rains during the evaluations, and the pressure drop of the Memtec filter decreased dramatically after passing clean, dry air through the filter and after the filter sat idle for one week. Subsequent laboratory tests on a single filter cartridge confirmed that water accumulation in the filter was responsible for the increase in filter pressure drop and the inability to lower the pressure drop by reverse air pulses. No effort was made to identify the source of the water accumulation and correct the problem because the available funds were exhausted.

  14. Post-Transurethral Resection of the Prostate Inflation of Pressure-Controlled Endorectal Balloon-Impact on Postoperative Bleeding: A Preliminary Experimental Pilot Study.

    Science.gov (United States)

    Mohyelden, Khaled; Ibrahim, Hamdy; Abdel-Kader, Osman; Sherief, Mahmoud H; El-Nashar, Ahmed; Shaker, Hosam; Elkoushy, Mohamed A

    2016-02-01

    To evaluate the impact of rectal balloon (RB) inflation on post-transurethral resection of the prostate (TURP) bleeding in patients with symptomatic benign prostatic hyperplasia. After institutional review board approval, patients who were eligible for TURP were randomized into two equal groups, depending on whether they received postoperative endorectal balloon (RB) (GII) or not (GI). The tip of three-way Foley catheter was fixed to a balloon by a blaster strip to prepare air-tight RB. Postoperatively, the RB was inflated for 15 minutes by a pressure-controlled sphygmomanometer. Perioperative data were compared between both groups, including hemoglobin (Hb) deficit 24-hour postoperatively and at time of discharge. Functional outcomes, anorectal complaints, and adverse events were assessed perioperatively and after 1 and 3 months. Fifty patients were enrolled, including 13 (26%) patients who presented with indwelling urethral catheters. Baseline data and mean resected tissue weight were comparable between both groups, including preoperative Hb (p = 0.17). Immediate postoperative Hb deficit was, comparable between GI and GII patients (0.58 ± 0.18 vs 0.60 ± 0.2, p = 0.56) before RB inflation, respectively. However, compared to GI patients, mean Hb deficit significantly decreased in GII patients 24-hour postoperatively (0.2 ± 0.2 vs 0.7 ± 0.3 g, p = 0.002) and at time of discharge (0.8 ± 0.2 vs 1.3 ± 0.4 g, p = 0.003). GII patients needed significantly less postoperative irrigation (2.1 ± 1.6 vs 8.3 ± 1.8 L, p hematuria or clot retention in either group, while there were no anorectal complaints reported by GII patients. Post-TURP endorectal balloon inflation seems to be simple, safe, and an efficient procedure to reduce postoperative bleeding and irrigation volume. It is significantly associated with shorter catheterization time and hospital stay.

  15. Study of wet blasting of components in nuclear power stations

    Energy Technology Data Exchange (ETDEWEB)

    Hall, J

    1999-12-01

    This report looks at the method of wet blasting radioactive components in nuclear power stations. The wet blaster uses pearl shaped glass beads with the dimensions of 150-250 {mu}m mixed with water as blasting media. The improved design, providing outer operator's positions with proper radiation protection and more efficient blasting equipment has resulted in a lesser dose taken by the operators. The main reason to decontaminate components in nuclear power plants is to enable service on these components. On components like valves, pump shafts, pipes etc. oxides form and bind radiation. These components are normally situated at some distance from the reactor core and will mainly suffer from radiation from so called activation products. When a component is to be decontaminated it can be decontaminated to a radioactive level where it will be declassified. This report has found levels ranging from 150-1000 Bq/kg allowing declassification of radioactive materials.This difference is found between different countries and different organisations. The report also looks at the levels of waste generated using wet blasting. This is done by tracking the contamination to determine where it collects. It is either collected in the water treatment plant or collected in the blasting media. At Barsebaeck the waste levels, from de-contaminating nearly 800 components in one year, results in a waste volume of about 0,250 m{sup 3}. This waste consists of low and medium level waste and will cost about 3 600 EURO to store. The conclusions of the report are that wet blasting is an indispensable way to treat contaminated components in modern nuclear power plants. The wet blasting equipment can be improved by using a robot enabling the operators to remotely treat components from the outer operator's positions. There they will benefit from better radiation protection thus further reduce their taken dose. The wet blasting equipment could also be used to better control the levels of

  16. istar: a web platform for large-scale protein-ligand docking.

    Directory of Open Access Journals (Sweden)

    Hongjian Li

    Full Text Available Protein-ligand docking is a key computational method in the design of starting points for the drug discovery process. We are motivated by the desire to automate large-scale docking using our popular docking engine idock and thus have developed a publicly-accessible web platform called istar. Without tedious software installation, users can submit jobs using our website. Our istar website supports 1 filtering ligands by desired molecular properties and previewing the number of ligands to dock, 2 monitoring job progress in real time, and 3 visualizing ligand conformations and outputting free energy and ligand efficiency predicted by idock, binding affinity predicted by RF-Score, putative hydrogen bonds, and supplier information for easy purchase, three useful features commonly lacked on other online docking platforms like DOCK Blaster or iScreen. We have collected 17,224,424 ligands from the All Clean subset of the ZINC database, and revamped our docking engine idock to version 2.0, further improving docking speed and accuracy, and integrating RF-Score as an alternative rescoring function. To compare idock 2.0 with the state-of-the-art AutoDock Vina 1.1.2, we have carried out a rescoring benchmark and a redocking benchmark on the 2,897 and 343 protein-ligand complexes of PDBbind v2012 refined set and CSAR NRC HiQ Set 24Sept2010 respectively, and an execution time benchmark on 12 diverse proteins and 3,000 ligands of different molecular weight. Results show that, under various scenarios, idock achieves comparable success rates while outperforming AutoDock Vina in terms of docking speed by at least 8.69 times and at most 37.51 times. When evaluated on the PDBbind v2012 core set, our istar platform combining with RF-Score manages to reproduce Pearson's correlation coefficient and Spearman's correlation coefficient of as high as 0.855 and 0.859 respectively between the experimental binding affinity and the predicted binding affinity of the docked

  17. istar: a web platform for large-scale protein-ligand docking.

    Science.gov (United States)

    Li, Hongjian; Leung, Kwong-Sak; Ballester, Pedro J; Wong, Man-Hon

    2014-01-01

    Protein-ligand docking is a key computational method in the design of starting points for the drug discovery process. We are motivated by the desire to automate large-scale docking using our popular docking engine idock and thus have developed a publicly-accessible web platform called istar. Without tedious software installation, users can submit jobs using our website. Our istar website supports 1) filtering ligands by desired molecular properties and previewing the number of ligands to dock, 2) monitoring job progress in real time, and 3) visualizing ligand conformations and outputting free energy and ligand efficiency predicted by idock, binding affinity predicted by RF-Score, putative hydrogen bonds, and supplier information for easy purchase, three useful features commonly lacked on other online docking platforms like DOCK Blaster or iScreen. We have collected 17,224,424 ligands from the All Clean subset of the ZINC database, and revamped our docking engine idock to version 2.0, further improving docking speed and accuracy, and integrating RF-Score as an alternative rescoring function. To compare idock 2.0 with the state-of-the-art AutoDock Vina 1.1.2, we have carried out a rescoring benchmark and a redocking benchmark on the 2,897 and 343 protein-ligand complexes of PDBbind v2012 refined set and CSAR NRC HiQ Set 24Sept2010 respectively, and an execution time benchmark on 12 diverse proteins and 3,000 ligands of different molecular weight. Results show that, under various scenarios, idock achieves comparable success rates while outperforming AutoDock Vina in terms of docking speed by at least 8.69 times and at most 37.51 times. When evaluated on the PDBbind v2012 core set, our istar platform combining with RF-Score manages to reproduce Pearson's correlation coefficient and Spearman's correlation coefficient of as high as 0.855 and 0.859 respectively between the experimental binding affinity and the predicted binding affinity of the docked conformation. istar

  18. Construction of a Recyclable Genetic Marker and Serial Gene Deletions in the Human Pathogenic Mucorales Mucor circinelloides.

    Science.gov (United States)

    Garcia, Alexis; Adedoyin, Gloria; Heitman, Joseph; Lee, Soo Chan

    2017-07-05

    Mucor circinelloides is a human pathogen, biofuel producer, and model system that belongs to a basal fungal lineage; however, the genetics of this fungus are limited. In contrast to ascomycetes and basidiomycetes, basal fungal lineages have been understudied. This may be caused by a lack of attention given to these fungi, as well as limited tools for genetic analysis. Nonetheless, the importance of these fungi as pathogens and model systems has increased. M. circinelloides is one of a few genetically tractable organisms in the basal fungi, but it is far from a robust genetic system when compared to model fungi in the subkingdom Dikarya. One problem is the organism is resistant to drugs utilized to select for dominant markers in other fungal transformation systems. Thus, we developed a blaster recyclable marker system by using the pyrG gene (encoding an orotidine-5'-phosphate decarboxylase, ortholog of URA3 in Saccharomyces cerevisiae ). A 237-bp fragment downstream of the pyrG gene was tandemly incorporated into the upstream region of the gene, resulting in construction of a pyrG-dpl237 marker. To test the functionality of the pyrG-dpl237 marker, we disrupted the carRP gene that is involved in carotenoid synthesis in pyrG - mutant background. The resulting carRP :: pyrG-dpl237 mutants exhibit a white colony phenotype due to lack of carotene, whereas wild type displays yellowish colonies. The pyrG marker was then successfully excised, generating carRP-dpl237 on 5-FOA medium. The mutants became auxotrophic and required uridine for growth. We then disrupted the calcineurin B regulatory subunit cnbR gene in the carRP :: dpl237 strain, generating mutants with the alleles carRP :: dpl237 and cnbR :: pyrG These results demonstrate that the recyclable marker system is fully functional, and therefore the pyrG-dpl237 marker can be used for sequential gene deletions in M. circinelloides . Copyright © 2017 Garcia et al.

  19. Construction of a Recyclable Genetic Marker and Serial Gene Deletions in the Human Pathogenic Mucorales Mucor circinelloides

    Directory of Open Access Journals (Sweden)

    Alexis Garcia

    2017-07-01

    Full Text Available Mucor circinelloides is a human pathogen, biofuel producer, and model system that belongs to a basal fungal lineage; however, the genetics of this fungus are limited. In contrast to ascomycetes and basidiomycetes, basal fungal lineages have been understudied. This may be caused by a lack of attention given to these fungi, as well as limited tools for genetic analysis. Nonetheless, the importance of these fungi as pathogens and model systems has increased. M. circinelloides is one of a few genetically tractable organisms in the basal fungi, but it is far from a robust genetic system when compared to model fungi in the subkingdom Dikarya. One problem is the organism is resistant to drugs utilized to select for dominant markers in other fungal transformation systems. Thus, we developed a blaster recyclable marker system by using the pyrG gene (encoding an orotidine-5′-phosphate decarboxylase, ortholog of URA3 in Saccharomyces cerevisiae. A 237-bp fragment downstream of the pyrG gene was tandemly incorporated into the upstream region of the gene, resulting in construction of a pyrG-dpl237 marker. To test the functionality of the pyrG-dpl237 marker, we disrupted the carRP gene that is involved in carotenoid synthesis in pyrG− mutant background. The resulting carRP::pyrG-dpl237 mutants exhibit a white colony phenotype due to lack of carotene, whereas wild type displays yellowish colonies. The pyrG marker was then successfully excised, generating carRP-dpl237 on 5-FOA medium. The mutants became auxotrophic and required uridine for growth. We then disrupted the calcineurin B regulatory subunit cnbR gene in the carRP::dpl237 strain, generating mutants with the alleles carRP::dpl237 and cnbR::pyrG. These results demonstrate that the recyclable marker system is fully functional, and therefore the pyrG-dpl237 marker can be used for sequential gene deletions in M. circinelloides.

  20. Combined evidence annotation of transposable elements in genome sequences.

    Directory of Open Access Journals (Sweden)

    Hadi Quesneville

    2005-07-01

    Full Text Available Transposable elements (TEs are mobile, repetitive sequences that make up significant fractions of metazoan genomes. Despite their near ubiquity and importance in genome and chromosome biology, most efforts to annotate TEs in genome sequences rely on the results of a single computational program, RepeatMasker. In contrast, recent advances in gene annotation indicate that high-quality gene models can be produced from combining multiple independent sources of computational evidence. To elevate the quality of TE annotations to a level comparable to that of gene models, we have developed a combined evidence-model TE annotation pipeline, analogous to systems used for gene annotation, by integrating results from multiple homology-based and de novo TE identification methods. As proof of principle, we have annotated "TE models" in Drosophila melanogaster Release 4 genomic sequences using the combined computational evidence derived from RepeatMasker, BLASTER, TBLASTX, all-by-all BLASTN, RECON, TE-HMM and the previous Release 3.1 annotation. Our system is designed for use with the Apollo genome annotation tool, allowing automatic results to be curated manually to produce reliable annotations. The euchromatic TE fraction of D. melanogaster is now estimated at 5.3% (cf. 3.86% in Release 3.1, and we found a substantially higher number of TEs (n = 6,013 than previously identified (n = 1,572. Most of the new TEs derive from small fragments of a few hundred nucleotides long and highly abundant families not previously annotated (e.g., INE-1. We also estimated that 518 TE copies (8.6% are inserted into at least one other TE, forming a nest of elements. The pipeline allows rapid and thorough annotation of even the most complex TE models, including highly deleted and/or nested elements such as those often found in heterochromatic sequences. Our pipeline can be easily adapted to other genome sequences, such as those of the D. melanogaster heterochromatin or other

  1. Demonstration of a Fiber Optic Regression Probe

    Science.gov (United States)

    Korman, Valentin; Polzin, Kurt A.

    2010-01-01

    empirically anchoring any analysis geared towards lifetime qualification. Erosion rate data over an operating envelope could also be useful in the modeling detailed physical processes. The sensor has been embedded in many regressing media for the purposes of proof-of-concept testing. A gross demonstration of its capabilities was performed using a sanding wheel to remove layers of metal. A longer-term demonstration measurement involved the placement of the sensor in a brake pad, monitoring the removal of pad material associated with the normal wear-and-tear of driving. It was used to measure the regression rates of the combustable media in small model rocket motors and road flares. Finally, a test was performed using a sand blaster to remove small amounts of material at a time. This test was aimed at demonstrating the unit's present resolution, and is compared with laser profilometry data obtained simultaneously. At the lowest resolution levels, this unit should be useful in locally quantifying the erosion rates of the channel walls in plasma thrusters. .